LOCUS       DAJOTB010000001        90543 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJOTB010000001 DAJOTB010000000
VERSION     DAJOTB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.001"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            111..386
                     /locus_tag="OHN28_000001"
     CDS             111..386
                     /locus_tag="OHN28_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295454.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            405..1436
                     /gene="mdtN"
                     /locus_tag="OHN28_000002"
     CDS             405..1436
                     /gene="mdtN"
                     /locus_tag="OHN28_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCQ2295455.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVSDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPKMFRIGA
                     SAVANLEPQ"
     gene            1436..3487
                     /gene="mdtO"
                     /locus_tag="OHN28_000003"
     CDS             1436..3487
                     /gene="mdtO"
                     /locus_tag="OHN28_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCQ2295456.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            3484..4950
                     /gene="mdtP"
                     /locus_tag="OHN28_000004"
     CDS             3484..4950
                     /gene="mdtP"
                     /locus_tag="OHN28_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCQ2295457.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLNGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAISAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            5148..7295
                     /locus_tag="OHN28_000005"
     CDS             5148..7295
                     /locus_tag="OHN28_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5565..5567,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HCQ2295458.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7670..9238
                     /locus_tag="OHN28_000006"
     CDS             7670..9238
                     /locus_tag="OHN28_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2295459.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            9338..10387
                     /locus_tag="OHN28_000007"
     CDS             9338..10387
                     /locus_tag="OHN28_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2295460.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            10387..11220
                     /locus_tag="OHN28_000008"
     CDS             10387..11220
                     /locus_tag="OHN28_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2295461.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            11213..12049
                     /locus_tag="OHN28_000009"
     CDS             11213..12049
                     /locus_tag="OHN28_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2295462.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            12036..12740
                     /locus_tag="OHN28_000010"
     CDS             12036..12740
                     /locus_tag="OHN28_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2295463.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            12851..13540
                     /locus_tag="OHN28_000011"
     CDS             12851..13540
                     /locus_tag="OHN28_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2295464.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14029..15342)
                     /gene="gltP"
                     /locus_tag="OHN28_000012"
     CDS             complement(14029..15342)
                     /gene="gltP"
                     /locus_tag="OHN28_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCQ2295465.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
     gene            complement(15685..16281)
                     /gene="nrfG"
                     /locus_tag="OHN28_000013"
     CDS             complement(15685..16281)
                     /gene="nrfG"
                     /locus_tag="OHN28_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCQ2295466.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(16278..16661)
                     /gene="nrfF"
                     /locus_tag="OHN28_000014"
     CDS             complement(16278..16661)
                     /gene="nrfF"
                     /locus_tag="OHN28_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCQ2295467.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(16654..18372)
                     /locus_tag="OHN28_000015"
     CDS             complement(16654..18372)
                     /locus_tag="OHN28_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCQ2295468.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(18392..19348)
                     /gene="nrfD"
                     /locus_tag="OHN28_000016"
     CDS             complement(18392..19348)
                     /gene="nrfD"
                     /locus_tag="OHN28_000016"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCQ2295469.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(19345..20016)
                     /gene="nrfC"
                     /locus_tag="OHN28_000017"
     CDS             complement(19345..20016)
                     /gene="nrfC"
                     /locus_tag="OHN28_000017"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCQ2295470.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(20013..20579)
                     /gene="nrfB"
                     /locus_tag="OHN28_000018"
     CDS             complement(20013..20579)
                     /gene="nrfB"
                     /locus_tag="OHN28_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCQ2295471.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(20624..22060)
                     /gene="nrfA"
                     /locus_tag="OHN28_000019"
     CDS             complement(20624..22060)
                     /gene="nrfA"
                     /locus_tag="OHN28_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCQ2295472.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            22452..24410
                     /gene="acs"
                     /locus_tag="OHN28_000020"
     CDS             22452..24410
                     /gene="acs"
                     /locus_tag="OHN28_000020"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCQ2295473.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            24620..24934
                     /locus_tag="OHN28_000021"
     CDS             24620..24934
                     /locus_tag="OHN28_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCQ2295474.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            24931..26580
                     /gene="actP"
                     /locus_tag="OHN28_000022"
     CDS             24931..26580
                     /gene="actP"
                     /locus_tag="OHN28_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCQ2295475.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(26619..27308)
                     /locus_tag="OHN28_000023"
     CDS             complement(26619..27308)
                     /locus_tag="OHN28_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCQ2295476.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(27301..27711)
                     /locus_tag="OHN28_000024"
     CDS             complement(27301..27711)
                     /locus_tag="OHN28_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCQ2295477.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27815..28699
                     /locus_tag="OHN28_000025"
     CDS             27815..28699
                     /locus_tag="OHN28_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2295478.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(28735..30384)
                     /locus_tag="OHN28_000026"
     CDS             complement(28735..30384)
                     /locus_tag="OHN28_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCQ2295479.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(30535..31884)
                     /gene="ghxP"
                     /locus_tag="OHN28_000027"
                     /gene_synonym="yjcD"
     CDS             complement(30535..31884)
                     /gene="ghxP"
                     /locus_tag="OHN28_000027"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCQ2295480.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(32431..32895)
                     /gene="soxR"
                     /locus_tag="OHN28_000028"
     CDS             complement(32431..32895)
                     /gene="soxR"
                     /locus_tag="OHN28_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCQ2295481.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            32981..33304
                     /gene="soxS"
                     /locus_tag="OHN28_000029"
     CDS             32981..33304
                     /gene="soxS"
                     /locus_tag="OHN28_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCQ2295482.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(33307..34893)
                     /gene="pdeC"
                     /locus_tag="OHN28_000030"
     CDS             complement(33307..34893)
                     /gene="pdeC"
                     /locus_tag="OHN28_000030"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCQ2295483.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLFLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     KLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            35322..35603
                     /locus_tag="OHN28_000031"
     CDS             35322..35603
                     /locus_tag="OHN28_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCQ2295484.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(35702..36238)
                     /gene="ssb1"
                     /locus_tag="OHN28_000032"
     CDS             complement(35702..36238)
                     /gene="ssb1"
                     /locus_tag="OHN28_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCQ2295485.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            36493..39315
                     /gene="uvrA"
                     /locus_tag="OHN28_000033"
     CDS             36493..39315
                     /gene="uvrA"
                     /locus_tag="OHN28_000033"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCQ2295486.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPDGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(39350..39706)
                     /locus_tag="OHN28_000034"
     CDS             complement(39350..39706)
                     /locus_tag="OHN28_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2295487.1"
                     /translation="MTILELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(39710..40126)
                     /locus_tag="OHN28_000035"
     CDS             complement(39710..40126)
                     /locus_tag="OHN28_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCQ2295488.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(40237..40950)
                     /gene="aphA"
                     /locus_tag="OHN28_000036"
     CDS             complement(40237..40950)
                     /gene="aphA"
                     /locus_tag="OHN28_000036"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCQ2295489.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            41227..43047
                     /locus_tag="OHN28_000037"
     CDS             41227..43047
                     /locus_tag="OHN28_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCQ2295490.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEKHE"
     gene            43040..44398
                     /locus_tag="OHN28_000038"
     CDS             43040..44398
                     /locus_tag="OHN28_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCQ2295491.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(44387..45460)
                     /locus_tag="OHN28_000039"
     CDS             complement(44387..45460)
                     /locus_tag="OHN28_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCQ2295492.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     TKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(45472..46971)
                     /locus_tag="OHN28_000040"
     CDS             complement(45472..46971)
                     /locus_tag="OHN28_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCQ2295493.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(47183..48055)
                     /gene="sucD"
                     /locus_tag="OHN28_000041"
     CDS             complement(47183..48055)
                     /gene="sucD"
                     /locus_tag="OHN28_000041"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCQ2295494.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            complement(48068..49237)
                     /gene="sucC"
                     /locus_tag="OHN28_000042"
     CDS             complement(48068..49237)
                     /gene="sucC"
                     /locus_tag="OHN28_000042"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCQ2295495.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            complement(49259..50677)
                     /gene="lpdA"
                     /locus_tag="OHN28_000043"
     CDS             complement(49259..50677)
                     /gene="lpdA"
                     /locus_tag="OHN28_000043"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCQ2295496.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLVVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(50689..51843)
                     /gene="odhB"
                     /locus_tag="OHN28_000044"
     CDS             complement(50689..51843)
                     /gene="odhB"
                     /locus_tag="OHN28_000044"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCQ2295497.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            complement(51876..54620)
                     /locus_tag="OHN28_000045"
     CDS             complement(51876..54620)
                     /locus_tag="OHN28_000045"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCQ2295498.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            complement(54802..55995)
                     /gene="tyrB"
                     /locus_tag="OHN28_000046"
     CDS             complement(54802..55995)
                     /gene="tyrB"
                     /locus_tag="OHN28_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCQ2295499.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            complement(56077..57081)
                     /locus_tag="OHN28_000047"
     CDS             complement(56077..57081)
                     /locus_tag="OHN28_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2295500.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            complement(57078..57635)
                     /locus_tag="OHN28_000048"
     CDS             complement(57078..57635)
                     /locus_tag="OHN28_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCQ2295501.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            complement(57658..58737)
                     /gene="alr"
                     /locus_tag="OHN28_000049"
     CDS             complement(57658..58737)
                     /gene="alr"
                     /locus_tag="OHN28_000049"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCQ2295502.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(58790..60205)
                     /gene="dnaB"
                     /locus_tag="OHN28_000050"
     CDS             complement(58790..60205)
                     /gene="dnaB"
                     /locus_tag="OHN28_000050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCQ2295503.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            complement(60278..61312)
                     /locus_tag="OHN28_000051"
     CDS             complement(60278..61312)
                     /locus_tag="OHN28_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2295504.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            61568..63139
                     /locus_tag="OHN28_000052"
     CDS             61568..63139
                     /locus_tag="OHN28_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2295505.1"
                     /translation="MCSVRTITAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERF
                     QRAGHPQNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQM
                     INDNAIPAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSL
                     VERVRFANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGG
                     IIIAQVKRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSA
                     FRYMEHGPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAI
                     GGVPLLEFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPAR
                     PHVTAGCGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVT
                     FSGKRAIRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDL
                     KLMNPLYFQQGSHHE"
     gene            63132..63911
                     /locus_tag="OHN28_000053"
     CDS             63132..63911
                     /locus_tag="OHN28_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCQ2295506.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            63938..65218
                     /locus_tag="OHN28_000054"
     CDS             63938..65218
                     /locus_tag="OHN28_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCQ2295507.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            65229..67265
                     /locus_tag="OHN28_000055"
     CDS             65229..67265
                     /locus_tag="OHN28_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCQ2295508.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            67371..68354
                     /locus_tag="OHN28_000056"
     CDS             67371..68354
                     /locus_tag="OHN28_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCQ2295509.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(68519..68761)
                     /gene="pspG"
                     /locus_tag="OHN28_000057"
     CDS             complement(68519..68761)
                     /gene="pspG"
                     /locus_tag="OHN28_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCQ2295510.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(68895..69890)
                     /gene="dusA"
                     /locus_tag="OHN28_000058"
     CDS             complement(68895..69890)
                     /gene="dusA"
                     /locus_tag="OHN28_000058"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCQ2295511.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            70046..70561
                     /gene="zur"
                     /locus_tag="OHN28_000059"
     CDS             70046..70561
                     /gene="zur"
                     /locus_tag="OHN28_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCQ2295512.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(70603..70812)
                     /locus_tag="OHN28_000060"
     CDS             complement(70603..70812)
                     /locus_tag="OHN28_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCQ2295513.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(70928..72253)
                     /gene="dinF"
                     /locus_tag="OHN28_000061"
     CDS             complement(70928..72253)
                     /gene="dinF"
                     /locus_tag="OHN28_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCQ2295514.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(72326..72934)
                     /gene="lexA"
                     /locus_tag="OHN28_000062"
     CDS             complement(72326..72934)
                     /gene="lexA"
                     /locus_tag="OHN28_000062"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCQ2295515.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(73044..73412)
                     /gene="dgkA"
                     /locus_tag="OHN28_000063"
     CDS             complement(73044..73412)
                     /gene="dgkA"
                     /locus_tag="OHN28_000063"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCQ2295516.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            73583..76006
                     /gene="plsB"
                     /locus_tag="OHN28_000064"
     CDS             73583..76006
                     /gene="plsB"
                     /locus_tag="OHN28_000064"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCQ2295517.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(76161..77033)
                     /gene="ubiA"
                     /locus_tag="OHN28_000065"
     CDS             complement(76161..77033)
                     /gene="ubiA"
                     /locus_tag="OHN28_000065"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCQ2295518.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(77046..77543)
                     /gene="ubiC"
                     /locus_tag="OHN28_000066"
     CDS             complement(77046..77543)
                     /gene="ubiC"
                     /locus_tag="OHN28_000066"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCQ2295519.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(77722..78642)
                     /gene="malM"
                     /locus_tag="OHN28_000067"
     CDS             complement(77722..78642)
                     /gene="malM"
                     /locus_tag="OHN28_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCQ2295520.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(78875..80215)
                     /gene="lamB"
                     /locus_tag="OHN28_000068"
     CDS             complement(78875..80215)
                     /gene="lamB"
                     /locus_tag="OHN28_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCQ2295521.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(80287..81402)
                     /gene="malK"
                     /locus_tag="OHN28_000069"
     CDS             complement(80287..81402)
                     /gene="malK"
                     /locus_tag="OHN28_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCQ2295522.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            81767..82957
                     /gene="malE"
                     /locus_tag="OHN28_000070"
     CDS             81767..82957
                     /gene="malE"
                     /locus_tag="OHN28_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCQ2295523.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            83111..84655
                     /gene="malF"
                     /locus_tag="OHN28_000071"
     CDS             83111..84655
                     /gene="malF"
                     /locus_tag="OHN28_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCQ2295524.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            84670..85560
                     /gene="malG"
                     /locus_tag="OHN28_000072"
     CDS             84670..85560
                     /gene="malG"
                     /locus_tag="OHN28_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCQ2295525.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(85654..86064)
                     /gene="psiE"
                     /locus_tag="OHN28_000073"
     CDS             complement(85654..86064)
                     /gene="psiE"
                     /locus_tag="OHN28_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCQ2295526.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            86279..86557
                     /locus_tag="OHN28_000074"
     CDS             86279..86557
                     /locus_tag="OHN28_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295527.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(86604..88700)
                     /locus_tag="OHN28_000075"
     CDS             complement(86604..88700)
                     /locus_tag="OHN28_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCQ2295528.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(88700..89437)
                     /locus_tag="OHN28_000076"
     CDS             complement(88700..89437)
                     /locus_tag="OHN28_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCQ2295529.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(89434..90072)
                     /locus_tag="OHN28_000077"
     CDS             complement(89434..90072)
                     /locus_tag="OHN28_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCQ2295530.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(90187..90429)
                     /locus_tag="OHN28_000078"
     CDS             complement(90187..90429)
                     /locus_tag="OHN28_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295531.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT        22244 a        24229 c        22852 g        21218 t
ORIGIN      
        1 tggctgaata aagctatttt tattcatcga gaaaatagcc atactgacgc cttcaatgga
       61 tattgatatt cactacagat attgttcgca gcagacagga tgtatttaat atgccaacag
      121 ttctctctcg catagcgatg caactcaaaa aaatagcctg gataattccc gtcttcatgg
      181 tttcgggatg ctcattatct ccggcaatcc cggtgatcgg cgcttattat cccggctggt
      241 ttttctgcgc cattgccagc cttattttga cgctcatcac gaggcgaatt attcagcgga
      301 caaatatcaa tctggcattt gtcggaatta tttataccgc cctttttgct ctctacgcca
      361 tgctgttctg gctggcattt ttctaatcaa tttgagacgc gctcatggaa agtacgccga
      421 aaaaagctcc tcgcagtaaa ttccctgctc tgttagtggt tgcgttggcg ctggttgccc
      481 ttgttttcgt tatctggcgc gtagacagtg cgccatcaac taatgacgct tacgcgtcag
      541 cagataccat tgatgtggtg ccggaagtca gcggtcgcat tgtagaactg gcggtcagcg
      601 acaaccaggc ggtgaaacag ggcgatttgc tgttccgcat tgacccgcgc ccgtacgaag
      661 ccaatctggc gaaagctgaa gcctccctcg cggcgctgga taagcaaatt atgctcaccc
      721 agcgtagcgt tgacgcgcaa cagtttggtg ccgactcggt taatgccacg gtagaaaaag
      781 cccgtgccgc cgcgaaacaa gccagcgata cgttacgccg taccgaaccg ctactgcgag
      841 aaggttttgt ttctgcggaa gaggttgacc gtgcaagaac cgcccagcgt gcggcagaag
      901 cggaccttaa tgccgtattg ttgcaggcgc agtcagccgc cagtgccgtc agcggcgtcg
      961 atgcgctggt cgcccaacgt gcggcggtcg aagcggatat tgccctgacc aaactgcatc
     1021 tggaaatggc gaccgttcgc gcgccgtttg atggccgggt catttccctc aaaacctccg
     1081 tcgggcaatt tgcttctgcc atgcgcccta ttttcaccct aatcgacact cgtcactggt
     1141 acgtgatcgc caatttccgc gaaactgatc tgaaaaatat tcgctcaggt acacccgcaa
     1201 cgattcgcct gatgagcgac agcggcaaaa ccttcgaggg taaagtggat tcgattggct
     1261 acggcgtgct accggatgac ggcggcctgg tgctgggcgg cctgccgaaa gtgtcgcgtt
     1321 ctattaactg ggtccgcgtt gcccagcgtt ttccggtcaa aatcatggtt gataaacctg
     1381 acccgaaaat gttccgcatc ggcgcttcgg cagtcgctaa tcttgagccg caataatgag
     1441 cgcgctcaac tccctgccat taccggtggt caggctgctg gcgttctttc atgaagagtt
     1501 aagcgagcgg cggccaggtc gtgtgccgca gaccgtgcaa ctctgggtag gctgcctact
     1561 ggtgattctg atctcgatga cttttgagat cccttttgtg gcgttatcgc tggcagtgct
     1621 gttttacggt attcagtcga acgcgtttta caccaaattt gtggcgatct tgtttgtggt
     1681 tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat acggcgaacc
     1741 gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt tgatgcgcac
     1801 ccatcggctg gggctggtct ttttcgccgt cgccattgtc gctatttacg ggcaaacctt
     1861 ccccgccatg ctcgactatc cggaagtggt cgtgcgctta accctgtggt gtatcgttgt
     1921 cggcctctac ccgacgttat tgatgacgtt aatcggcgtg ctgtggtttc ccagccgcgc
     1981 tatcacgcag atgcatcagg cgcttaatga tcggcttgat gatgccatta gccacctgac
     2041 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     2101 actcaatgtc ttttgcctcg cggacgatgc caactggcga actcagagcg catggtggca
     2161 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     2221 ttttgctgat tctcaggcaa ttattgaatt ccgacaaaaa ttagcctcag aaatcaacaa
     2281 actgcagcat gccattgccg aaggtcagtg ctggcaaagc gactggcgga tcactgaaag
     2341 tgaagcgatg gcggcacggg aatgtaacct ggagaatatc tgccagacat tgttgcaact
     2401 gggtcagatg gacccgaata cgccgccaac gcccgccacc aaaccgccat caatagtcgc
     2461 cgacgctttt accaatccag actatatgcg ctacgcggta aaaacgctgc tcgcctgttt
     2521 gatctgttac accttctaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2581 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2641 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2701 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tgggcgcatg
     2761 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2821 gctcgccacg ctcgaaaacg tttttggccc ggtgtacgac ctggtggaaa ttcgcgatcg
     2881 cgctctgggt atcatcattg gtaccgtggt gtcggcggtg atttacacct ttgtctggcc
     2941 tgaaagcgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttgagtaa
     3001 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     3061 cataggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggtgctgga
     3121 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     3181 tcagggccgc gatattcttc acgcctggga tgccacctgg aactcggcgc aggcgctgga
     3241 taacgcacta cagccggaca gagcaggtca gtttgccgac gcactggaga aatacgccgc
     3301 tggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaaa cacccgcctc
     3361 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3421 cttgccagac tggacagccc cggcattaac gcccgccaag gaacaggcac aaggagccac
     3481 acaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3541 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3601 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3661 tggtggaaac aactcaatga cccgcagctg gatgcgctga tccaacggac tctaaatggt
     3721 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcagtccca ggccgatttg
     3781 ttagatgccg gttcacaatt acaggtcgca gcgttaggga tgctcaaccg ccaacgcgtc
     3841 tcggcgaacg gctttttaag cccttatgcg atggatgcgc cagcactggg tatggacggg
     3901 ccgtactata cggaagccac agtcggtttg tttgccggac tggatctcga tttgtggggt
     3961 gtgcatcgct cagcggttgc tgccgccatt agcgcgcata atgccgcgct ggcagaaacc
     4021 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     4081 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     4141 gcgcaccaga gtaaagtggc gcacggtctg gaagcgcaag tgcctttcca cggcgcgcgg
     4201 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     4261 cgagaatctc tgcgtgcatt gattggcgcg ggagccagcg atatgccgga gatcaaaccg
     4321 gtggcattac cgcaagtcca gaccggtatt ccggcgacac tctcttatga gttgctcgcc
     4381 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4441 tccgcgcggg cgctgttcta tccgagcttt gatatcaaag cgtttttcgg cctggattct
     4501 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaactttat cccaggtctg
     4561 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac tcgcgccgcc
     4621 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4681 gtcaacggca cacgtctgca aacgctcaac gacgagcgag agatgcaggc tgaacgcgta
     4741 gaagcaacgc gcttcaccca gcgcgcagcc gaggccgcct accaacgcgg cttaaccagc
     4801 cgcttacagg ccaccgaagc ccggttgcca gtgcttgccg aagagatgtc attactgatg
     4861 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4921 caggcggctc ccgtcgtcga gaaaaaataa aatgtctacc gcgtgatggc tgtcacgcgg
     4981 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     5041 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     5101 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     5161 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     5221 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     5281 gttattatgg ctgggacttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5341 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgggat gaggcactga
     5401 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5461 cgaccggctc ctcgcgcggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5521 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5581 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5641 ataataccga tttagtgttc gttttcgggt ataacccggc ggattcccac ccaatcgtgg
     5701 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5761 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5821 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5881 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5941 agtcggttga agatatcacc ggcgtcagcg ccagtgagat tcgtcaggcg gcgcggatgt
     6001 atgcccaggc agaaagcgcc gccatcctgt ggggcatggg ggtaacccag ttctatcagg
     6061 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccggtaac ctcggtaagc
     6121 cacatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     6181 gcgcgctgcc ggatacgtat ccgggctatc agtacgtgaa agatccggct aaccgcgaga
     6241 aatttgccaa agcctggggc gtagagagcc tgccagcgca taccggttat cgtatcagcg
     6301 agctgccgca ccgcgtggcg catggtgaag tgagagcggc gtacattatg ggcgaagatc
     6361 cgctgcaaac tgacgcggaa ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6421 ttatcgttca ggacatcttt atgaccaaaa ccgcgtcggc ggcggatgtc attttaccgt
     6481 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6541 tcttcaaggc ggttgagccg aagtgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6601 ttgccactcg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6661 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6721 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6781 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6841 caatcgacaa actcaccgac gaatacccga tggtactgtc aacggtgcgt gaagttggtc
     6901 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6961 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     7021 tggtttgggt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     7081 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     7141 tgaccgaaaa cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     7201 tcgagccgat cgccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     7261 tgaaaactcg cctgcgcgaa gcggcactgg cgtaataccg cttaaactca cgttaaaagc
     7321 ggtgatatta cccaacgcct ttgtatcaat ccggaaacga ctctctgttt ccggattttt
     7381 ttatacatac ctgcgcagtc aaaccagcct atcctggtta ttagcgacgt cttctcggtt
     7441 gttcttctat tccagtcacg attgcaaatg agaattatct ttactcaatt ctcatcgcgg
     7501 cctaacgtaa taatacttta catttgatga accaaatttc ggtaaggtga gaaatcatcg
     7561 accgtttaac tcgttctatt aacgggatat tttggcgcga ttaatgcaac aaaaccccgt
     7621 ctgatagaat attaataacc tcaaagtaat ttcagggatt gggaagcata tgattaaaaa
     7681 actattacct ttactggttt taagcacaat ttccgccaca accgttgccg ctacaccgcc
     7741 taatacgttg gttgtagccc aggggctgga tgatattgtg agcctggacc cggcagaagc
     7801 taatgaactc tcaagtattc agactgtacc aagtctgtat caacgcgtag tgcaaccgga
     7861 ccgtaatgat ccgacgaaaa ttactccgat tctggcggaa agttggcagg ctgaccccgc
     7921 agccaaaacg ctaaccataa aactgaaaag cgatgcgaaa tttgcctctg gtaatccggt
     7981 acgcccggaa gatgtgattt actcttatgt ccgggctgtc acactgaata aatccggtgc
     8041 gtttattctc aatgtgctgg gatggcagcc ggaaaatatc gccagccagt tgaagaaaat
     8101 cgatgaccat accgtccagg tgcaatggag tgccgacgtc agcccggctc tggcactgaa
     8161 tattctctcc acaccaatcg cctcaattgt tgacgagaag ctggttgcgc caaacgccaa
     8221 aaataacgac tttggtaatg aatggctgaa gatgcattcc gctggcagcg gcgcgtttaa
     8281 aatgcgtact taccaaccgc atcaggccat cgtgatggaa gccaacgcca gctcaccaac
     8341 cggcgccccg aaactgaaaa gcgtcatcat caaaaatgtt cccgatccgg caacacgtcg
     8401 cctgctgatc caacaaggtg atgctgatat ggctcgtgat ctgggagccg atcaaatcga
     8461 tgcattacag ggtaaacctg gcgtaaaagt gatgagcatt gcctccgccg aacagaacta
     8521 cctggccttc aataccggta acaaggataa cccgctgatg agtaatcctg cgttctggga
     8581 ggccgctcgc tggttggtcg attacgacgg catcaccaaa aatctcttaa aaggacaata
     8641 ttttacccat cagagtttcc tgcccgtggg ctttcctgga gcactggaag aaacaccgtt
     8701 tacctttaat ccagccaaag caaaagagat cctcgctaaa gccggaatca aagacccgca
     8761 cttcacgctt gatgtcgaaa ataaaccgcc atttattacc atcgcgcagt caattcaggc
     8821 cagctttgct cagggcggtg taaaagttga tttgctgcca gccgcaggga gtcaggtcta
     8881 ctctcgcgtt cgcgcccacc agcatcaggg ggccattcgt atgtggttgc ctgattattt
     8941 tgacgcacat tccaacgcca gctctttcgc ctataacgat ggcaaatcca gcaccgttgc
     9001 cgggttaaac ggctggaaaa tcccggaact gaacaaagaa acgctggcgg ccatcgctga
     9061 acctgatagc actaaacgtc ttgatttgta taagaaaatg caacaagaac tacaacgtag
     9121 ttccccgtat gtctttattg atcaaggcaa aacgcaaatt gtcatgcgtg ataatgtgca
     9181 ggggtatcaa caagggttga atgccgatat ggtctggttt gatcaggtaa ccaaataacc
     9241 ctgtatgcaa ctcgcgtcca gccatgttac cgggcgcggt accagggctg gcctgtattg
     9301 caggccagcc gctgatttat cactgtatgg aaagatcatg tctgtcgttt tttccactcg
     9361 cgcaagatgg cgaggcaaac gccctctgct cgcgctgttg caagggctgt ttaccgttgc
     9421 tctgacgcta ctcggcctgc tgctgattac ctttgcgctt tcggctctct caccggtcga
     9481 tcgcgtacta caaatcgttg gcgatcacgc cagccagtcc acctatgatc aggtacgcca
     9541 ccagttagga ttggatcagc cgttagccgt ccagttctgg cattacctga tgaatctggc
     9601 acacggcgat ctgggcatcg ccagctccac cgggcaacca gtactccagg acttgctatc
     9661 agcattcccg gcgaccattg aactggcaac gttggcgctt attgtcggtg ctgttgtcgg
     9721 cgtcattgcg ggtgtacttt gcgcccgtta tgtcggttct ccgctggatt tcaccgtgcg
     9781 aacattaact ctactgggaa attccgtccc tgttttttgg cttggtctgc tgatgctggc
     9841 gctgttctac gccagactgc aatggagtgc cgggccgggc aggctggatg atatttatca
     9901 attcaccata gaaccccgca gcggatttgc gctgattgat acgtggcttt ctggcgatcg
     9961 cgaggcattt accaatgcaa tcaaccattt gatacttccg gttttattac tggcgtactt
    10021 ctcgctcgcc agtattacgc gcctgactcg ttccgcctgt cttggcgaaa tgaataaaga
    10081 atatattctg ctggcgcgcg ccaaaggggc cagcgagatg acaattttgc tacgtcatgt
    10141 tttacccaac attcgcggca ccttgctcac cgttatcgcc ctcgcctata catcaatgct
    10201 ggaaggcgcg gtactgacag aaaccgtctt ttcatggcca ggtattgggc gctacctcac
    10261 tacagcgctc ttcgcgggtg ataccacagc aatcatggga ggaacgctgc tgattggcgt
    10321 ctgctttgtg ttgataaata acttaaccga cctgctggtc cggctgaccg atccgaggtt
    10381 acgctgatgc cgatgtttct ctttttacgc cgtttacgcc attcacccgc cgcgttttgc
    10441 gggttgataa ttatcctgtt attgatgcta attgcgctct tcgcgccgtg gctggcacct
    10501 cacgatccta actggcagga tgcggcggcg cgattacaag caccgagtcg tcagcactgg
    10561 ctcggcactg acagttacgg gcgagaccta ctttcacgtt taatctacgg cagccgcccg
    10621 gcactggggc tggtggcatt ggtgacaatc attacactcc cggtcggtct gctgattggc
    10681 attttagccg ggtactacgg cggctggctg gaacgcgtgc tgatgcgttt tactgacata
    10741 gtaatgtcaa tgccgcgttt aattctggct tttgcttttg ttgccatgct ggggccgggg
    10801 ctggttaacg gcgcactggc ccttgcatta accacctggc ctgcttatgc gcgtcaggca
    10861 cggagtgaga tccaacgtct gcgacacagt gattatcttg ccgccgcaga aatgatgggc
    10921 atccgcggca ggcgtctgct ggctggtcat attttgccat tgtgcctgcc atctgccatt
    10981 gtccgtctgg cgctggattt agcgggtatc attctggcgg ctgctggtct gggttttctt
    11041 gggcttggtg ctcgcccgcc aatggcggaa tggggagcca tgatcgccga cggtatgcag
    11101 gtcatttttg atcaatggtg gattgccgcc gctcccggcg caattatctt aattgccagt
    11161 ctggccttta atctgctggg tgatggcttg cgcgatgttc tggagcctca acatgactga
    11221 ctcacgtctt agtatcaaca atctctgcgt cgattatccg gagtcccggg tcgtcaacaa
    11281 tgtcagtttt acattaggca atgaacgtct ggcgctggtc ggtgagtcgg gttcaggtaa
    11341 gtccatgacc gcccgcgccc tgatggggct ggtgcgtaaa ccgggtgtag taaccgcgga
    11401 cacacttaac attctggggc gtgatgcact cacccttaac gcgcgtggat ggcaacagtt
    11461 gcgcggtaac gatatcgcga tggtattgca agatccgcgt tacgcgctta atccagtaaa
    11521 gacagtaaaa gcgcaactgg aagaggcgct tacgctccat caacgtctga accgccgcga
    11581 gaaagaagag aaaataaaca ccgcaattca ggctgtcggc ctcaatacgc gagtactgca
    11641 aagctatcct cgtgaacttt ctggcggcat ggggcagcga gtgatgattg caattgcgct
    11701 catcaacgat ccccgggtat tgattgccga tgagccaaca tcagcactag atgtgcgcct
    11761 gcgtaatcag attctggaac tgctggttac ccagtgtgaa caacgtcaga tggcaatgtt
    11821 gctgatcagt cacgacctgc cattagttgc ccaattttgc catcgggtgc tggtgatgta
    11881 tcagggaaat aaggtggatg aaatgcacgc tgctgcgcta cctacagcaa cgcatcctta
    11941 cacgcgcaca ctgtggactt gccgcccaaa tgcacaaact tacgggcaga tgctgccgac
    12001 gctggacagg acggcaatga cgccggagaa ataccatgac gattgttgaa attaatcact
    12061 tacaggtgac ttttgctgag aaaaccgcag tttctgccgc cagtttttcg gttaatgccg
    12121 gagagacatt tagtttaatt ggcgaatcag gctgcggaaa atccacgatt ttgcgcgtaa
    12181 ttgctggatt acagcgcgac tggcacggtc aggtgtcact gtttgggcac accatcaaac
    12241 caggaatgcg ttttcagggc gatctccgcc gcaatgtgca aatggtgttt caggatccct
    12301 atgcctcgtt gcatcccaat catactctgt ggcgaacgct ggctgaacca ctcaaaattc
    12361 gtggcgaaag agaaatagaa aaacgcgtcc agactgcact ggaacaggtt ggcttgcctt
    12421 tcgacgccgc tcgtcgctac ccacaccagc tttccggtgg tcaacggcaa cgtgtggtta
    12481 ttgctcgcgc cctgttgtta cgaccacaac ttcttctgct ggatgaaccc acctcggcgt
    12541 tggatatgtc ggtacaagcg gaaatcctta atttgttgaa tcaacttaag ctggaacacc
    12601 aaatgacata tctgctggtc agtcacgatg ccgatgtcat cgcgcatatg tctgatcgag
    12661 cggcattgat gtccgaggga aaaattcagc gattttttga tcgcgacgcc atggaaaagg
    12721 gggaacatcg gatggattaa aaaacctgac attgcattaa ttcattctgc tttcaggtct
    12781 ttttacagtt gtttttttgt ccacccgaac actttatact gtctctcccc taccaaactt
    12841 cattatcaaa atgaaaaaaa ttatcacttt gatgttgttt ttgacattct ttgcccacgc
    12901 gaacgactcc gagcctggca gccagtattt aaaggcagca gaggccggag accgacgcgc
    12961 acaatatttt cttgccgaca gctggtttag ctccggcgat ttgagcaaag ccgaatattg
    13021 ggcacagaaa gccgccgaca gcggtgatgc cgatgcctgc gcgctgttgg cgcagatcaa
    13081 aatcaccaat ccggtcagtc tggactatcc acaagcaaaa gtgcttgccg agaaagcagc
    13141 gcaagcgggc agtaaagaag gtgaagtaac gctggcgcat attctggtaa atacgcaagc
    13201 gggtaaaccg gattatccaa aggcgatttc gctgttagaa aacgcctcgg aagatctgga
    13261 gaacgactct gccgtcgatg cccaaatgct gcttggtttg atttacgcca acggcgtggg
    13321 cattaaggcc gacgatgaca aagcaacctg gtatttcaaa cgtagctctg cgatttcccg
    13381 aaccggttat tccgaatact gggcgggaat gatgttctta aacggtgaag agggttttat
    13441 cgagaagaat aaacaaaagg cgctgcactg gttgaatctg agctgtatgg aaggatttga
    13501 taccggttgt gaagagtttg aaaaattaac taacggttaa ggtaggcgca gcaaacgtcg
    13561 catcaggcat tgatgtcgga tgcggcgtaa acgccttatc cgacctacaa aaatcacgcc
    13621 aaatcaggct attgcgctgt ctgacgtagg cctgataaga cacgtcagca tcgcatcaga
    13681 cattgatgcc ggatgcggcg taaacgcctt atccgaccta caaaaatcac gccaaatcag
    13741 gctattgcgc tgtctggcgt aggcctgata agacgcggca agcgtcgcat caggcgttga
    13801 tgtcggatgc ggcgtaaacg ccttatccga cctacaaaaa tcacgccaaa tcaggctatt
    13861 gcgctgtctg gcgtaggcct gataagacgc gttaagcgtc gcatcaggcg ttgatgtcgg
    13921 atgcggcgta aacgccttat ccgacctaca aaaatcacgc tacatcaggc tattactcag
    13981 tcaggcatcc acacattgcc gggtggatat ccccccggca atcttcaatt actgatctgc
    14041 tgttttatca aatttgccca acatttcacg ctcgtaggcc agcgcttttt tacggtcaaa
    14101 tttgtgttcc cacttggcaa tcaccagcac cgccagcgca ttacccacca cgttcagcgc
    14161 agtacgcgcc atatcgagga tacggtcaac accggcaata aacgccagac cttccagcgg
    14221 aatacctacg ctacccagcg ttgccagcag caccacaaac gacacgcccg gcacgccagc
    14281 aatccctttc gaggtcacca tcagcgtcag caccagaatg atttcctgcc agatggacag
    14341 gtcaatgcca tacagctgag cgatgaaaat agcggcaata ctttgataca gcgtcgaacc
    14401 atcaaggtta aaagagtaac cggtcggcac cacgaaactg gtgatcgacg ccggtgcacc
    14461 gtaggcttcc atcttctcaa taattcgcgg cagcacgctt tcagaactgg cagtagagta
    14521 cgccagaatc agctcatctt tcagaatacg aatcaggatc cagacactta acccgcacag
    14581 gcgcgccaca attcccagta cgaccagcgc gaagaacaga atggcgaaat gcaccagcag
    14641 caccagtttc gccagtggcc acagagaaga gaaaccaaag ttagccaccg tcaccgcaat
    14701 cagcgcaaac acacccaccg gcgcataacg catcaccatg tgagtcactt taaacatggt
    14761 ttcagagatg gagcggaaaa cggtcaccag cggttcacga tgcgtcgcgg gcagggatga
    14821 aagccccaga ccaaacagca ccgagaagaa gatgatcggc agcatttcgc ctttcgccat
    14881 cgacgccacg atgttcgtcg gcaccagcga caaaatcgtg cccataatgc cgtgggaact
    14941 gctttgtacc gcttccgtag tgctctgata tttcgagata tcgacggtcg ccagctgcga
    15001 catatccacc ccggcaccgg gctggaagac gttcgccaga gtgatcccca aaatgatggc
    15061 gacggtggtg atcacctcga agtagataat ggttttcgca ccaatacgcc cgagctgttt
    15121 ggcatcacca acacccgcga tacccaccac cagcgtggag atcacaatcg gcacaacaat
    15181 cattttgatc agatggatga agatatcacc cgccggagag agcaaattga cgaccagcca
    15241 gtcgcggctg tcgctatggt agtgcaggta gcttcccagg agaatgccca gcaccatagc
    15301 aaacagaatc tgccaggcca ggctgaattt tatatttttc atcagaataa cttcctcaat
    15361 ggagcgccgt tccccgttca gaaacgcgag aacgtgacat actgccaggg catgaattgt
    15421 gttgcgttgg tttgtagatt tttttaacgc ttatgatcag tatcatttgc gcggtatgtt
    15481 gcgcaaccct ttaacaacag agcttttcgc tgcattaatg cataataacg cgagtttatg
    15541 gcaggattaa tgaataacca tttgttatag aaggattttt atttgtttaa tctgcaataa
    15601 tactgattag tgattatttt tgattatatg ttcattcgat tattttttat gcagctcaat
    15661 aacataatct tcatcaccag atgattaatc tgatctccgc tgcaacaatt tcgccatatt
    15721 aatcgactcg accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg
    15781 ccatagttcg atggcttgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat
    15841 cagagcggtg atttcgttag agtccagcgc gagggctttg tcgatcattg cgcgagcctg
    15901 gacggtcata tgttggctgg cctggtaata cagcaccgtc gccagcgccg catacagttc
    15961 agcgttctca ccacgtagtt gcaacgcctg acggtacgcc agcagcgaat tgctgtaatc
    16021 gttttgccac agataatact cgcccagtaa cgcccactgt tcgctgtttt gtggattagc
    16081 acggatttta tcctgcaatg cctgaagctg cgcttccggg gtttgctggc tggcaaactg
    16141 atgcagcgga tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttggacttaa
    16201 cagataactg ccgacacaga gaaataccat caaaatcgtg agtgttgtca gcattttcac
    16261 cggtatttgg ggctgtttca tcgcttcgcc ctcactcgcc agaggatcag tgccatcagc
    16321 agtaacaaca ctactggcag cgcccacaac accagagtct gacccgttaa cggtgggtta
    16381 tagcgaacaa aatctccgta gcgttcggtc atccagccaa tgatttcgac ttcacttttc
    16441 ccctcttcca ccatggtgta aacctgatgg cgcatactga ctgccaccgg cgcattggat
    16501 tccagtaagt tctgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc
    16561 tgttcctgtt gttgcggatt agcgaattgc caggtgtcta caacctgagc gcgcgcaaaa
    16621 caggtaaata acagcagtag cgtgagaagc cctttattca tcgcgcttcc ttccccgcca
    16681 tccgcttaac aatgcgcccg caatcatcaa caatcctccc ccccagatcc agcgcacacc
    16741 gctttgtaca tacaaacgaa atgcgtaacg atccgctccg gttttctctc ccatgaccgc
    16801 ataccagtca tggatgccgt tccagcgaat cgagggttcc atcatttgct gacggcgtgc
    16861 ttcgtaaaaa cgccgctccg gcgttaattc accaatgcgt tgctgatggt caaacagtgc
    16921 cactatcgct ttttcgctgg tgtaattgcc tttggcttgc agatcgaggc gctcaaaacg
    16981 gaaggtgtat cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg
    17041 acggctgacg ctggaaacca cgatccccgc ggcaaataac agcacgcccg catgcgccag
    17101 cagcgtcgga agccgcgcgc gtttgccaga gacaaacgtc gccagcacaa tcaccaccag
    17161 catcaacaga gcaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa cggagaggcg
    17221 tccccagccc agcaggccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag
    17281 cactgcgcaa aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa
    17341 acgcaccgcc gggccaccgt cccgcgctcg ccagccatac agagccagag acgcaaggct
    17401 aatcagtaca aacaggccga acaacggcac ggcgcggacg ttatccagcg cgaacgcatg
    17461 aaccgaaacc agaatgccag aacggacaat caacgtgccc agcagcgaca gcatcagagt
    17521 aactatcgtc agcaacagcg accagtggcg gaaaatcccc cgctggcgtg tcagggataa
    17581 actgtgcagc agcgctgtgg cagaaagcca gggtagtaaa gaggcgtttt ccaccggatc
    17641 ccagaaccac cagccgcccc agcccagttc gcaataggcc caccaggaac cgaggatgat
    17701 ccccgctgtt aatgcgctcc agccaggtag tgcccaacgc cagcaaattc gggcgcaggt
    17761 accatcaaac tcgccgcgca gtaaactcgc cagcgccacg ctcgccgcca ccatcaaacc
    17821 gccgtagccg agataaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg
    17881 attgagatcg cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag
    17941 caccacaaac agtagcagtg cggcgagcat gagagataaa acggctagcg tcagcggaaa
    18001 tagcggatcg gtttgctgcc ggtaatgcca ggcaaacagc gcgctccagg cggaaagcag
    18061 cagcacccaa agcaacagcg aaccttcatg accgccccac accgccgcca gtttgagttc
    18121 ccacgacagc aggctatagc tatgttgggc gacataaata accgagaaat cgctgatgag
    18181 aaaacaatac gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg ccagaatgcc
    18241 gatgcaagtg agtcgcatca tggcaggcca gcgcaaccgc actcccgcga aggccgtcaa
    18301 cgggatcaac acgttgaccc cgagacttaa caacaacgcc agaaaaccga cttcaggaag
    18361 gaatacgtcc agaaaccacc tctgttctgg cttacgccac cgttaactgc ccggcgtaga
    18421 gaatgaaaaa gcgcagcatc aacacgccga ccaggctggc cccacatgcc gccagcacgg
    18481 caggaatgcc ggaactgcga ttgacccacg gtttgagcaa cattggcaca atcagcccca
    18541 gcccggcgac accaagccag aaccaccacg tccagaaacc gccgcccaat gccgccacca
    18601 gcgcacgcac tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc accagcagga
    18661 agatttcacc ccataccacg gggatttcca tacggtgtac aaagtgcgct tccgtggaat
    18721 gcgggttact gcgttggcgt atcgccatgg cgatcagcgc caccgccgca ccggacgaga
    18781 tgccggagaa gaggaacagc accggcagga tcgggttatt gaggaacgga tacgatttca
    18841 gcgccgacag cagaaagccg gtataagccc ccaacaacac cgccagcacc agcatcaatg
    18901 tttccagtcc gcgatgaacg ggcgtcagca ggctcaagac cttttgcacg atccccagct
    18961 tcggcaacca gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca
    19021 gcaccagcac caccatgtaa agctgaaaca gcatcacccc catcgacatc accgaggtaa
    19081 aactgtagtg gaacatcagc ttccagaagg tccacggtct tgtcaggtgg aagacgagga
    19141 tcaacagacc gaggatcacc gcgccaggcc cgacaatcag cgtggtgcgc agcaacgtac
    19201 tgtctgcacc gcctgcctgc gggtagaagc gacgtaacag cacggccagt gtcaccagac
    19261 cggcagaaat accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt
    19321 caaaatgaaa tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa acggtacgcg
    19381 gtacagcttc ggtttggtgc ccagcgccag cttgtagcgg taagtgggct tctggcgcag
    19441 cagttgcgaa atctcactgt tgggatcgtc cagattgcca aacgtcagcg ccttcgtcgg
    19501 gcaagcttca acgcacgcgg gcagcttacc ggcctgcaaa tgggtcttac ggcagaaatc
    19561 gcatttgtcc gccgtcttcg tgaccggatg gataaagcgc acgcggtacg gacaggcggc
    19621 gatgcagtac tgacaaccga cgcagagatc cggattaaca tcgacaatcc cactggcagc
    19681 gtcgcgaaaa gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg
    19741 gcaagactta cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg
    19801 aataatcgtc aggcgcgaga cgccttccgg caccttgttc actttccgac aagcatccat
    19861 acaggcggta cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt
    19921 gatattcaac gttttcgcca ccacgcgccc tgcggtgccg ctgacggcgg ccagcacacc
    19981 gacgccggtg agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca
    20041 gacgccgggt taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta
    20101 atccgtcctt tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag
    20161 ctggcgcacg ccactttggt tacgtggaca tcgtgcggcc agaatgcttt ttgcaactgt
    20221 tccggcagat ggcaggacat acagacgctg ttctgctctc caaccttgta catcggctcg
    20281 ttaaagcgca tcacatcttt caccccttcg cggtgttgcg gtgatggctg gccgtgacag
    20341 ttggtgcagg tgaccggcag tttgttattc gggttgataa cggaagcatg tttgccatgc
    20401 ataccttcgg tatctggctt atgacagtcc agacaggcgg catccgggtt acgctgctgg
    20461 gtaacttcgt agcgatcacc agatgcctgc gccgcagggg tagcggtaat tccgttcagg
    20521 ctccacaaca ggcccgacgc cagcaccccg gcagttaata acgaacgtaa tacgctcata
    20581 ttcactccat tgtgttttcc gccccttgcg aggcggaacg gggttattgg cttaacagac
    20641 cgtttttacg tgcctgctct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct
    20701 cggccttgat ttgctccatg ttcaggccaa tggcctgctg ggctttctct ttggtcgaga
    20761 tatccgggat ctggatttca tgggtaatgc ctttggtcgc cagcaggcgc gccagtttgg
    20821 tgcgtgcatc cgccgcttta tccatcgccg taccgagcat cctcagacct tcttccggtg
    20881 cgtgcatatg aatgccgtgg gaagcgatcg ccaaatccca acgccactgg gcatgacgga
    20941 tatcgtcctg aatcggcttc atttcggctt ccgttgcgcc tgcatccagc gccgctttcg
    21001 cttcgaagtg agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgact
    21061 gcttacgttc cgcgaccact ttttgcaagg cagctttgtc ctgggtatgg cagttcgcac
    21121 aagtctgggc gaagttatca aacggattac caattttatg gtcggtgtag agtttgcctt
    21181 cggcgttctg cacttttggc atatggcaat cgatacaggt cacgttgttt ttgccgtgaa
    21241 tgcccgctgt ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca
    21301 gggagttagt ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt
    21361 tcatgccgtc atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca
    21421 catggcactg accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt
    21481 taccaatggc ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc
    21541 ctttggcgaa ctctggtgag gcggtgttat ggcaatcggc acaacctaag ttgttgacga
    21601 tttccgggcc gccgcgcgcc catttaccgt ggaagtagcc atcttcgccg tctttctgga
    21661 tcagacgcgc cacatccggg cttttacaac tccagcacgc catcggtagc ggaccatctt
    21721 cagcgttttt cggcgcaccg gtacgcaagg tttcacgcac atcggtcaca gcaaaggcat
    21781 gtccacgcgg cttgttgtaa tcgcgcgaga agggataccc cgcccacagg atcaccagcc
    21841 gtggatcttc cgccagggcg tcaacacgct ctgactgttc cgaggtagct ttccaggaga
    21901 gatattgatc gggatgctgc ggggcaaagg tttcattctt cgcttccaca gttacaggtg
    21961 ttgcgggagc agccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata
    22021 agctgaagat acggcgtgcg tttattttta tccttgtcat agggcttcat ccgaattgag
    22081 ccattgttgc aatggcggtt tttattgttt ttcacgacag taaccgcacc tacactgtca
    22141 tgacattgct cgcccctatg tgtaacaaat aaccacacta tgaatgttgt ctttaatcaa
    22201 ttgtaagtgc atgtaaaata ccactttaga gttagtcggt atcttcctct ttttcaacaa
    22261 catgcataac tgcatgttcc tcaaagaatt aatcaacttt tgttgctgac cttcaaaaat
    22321 taccctgccg tttatttgca caattctact tttgcgtgat ctgtcgccca aatactaaac
    22381 aaaactgcca atacccctac atttaacgct tatgccacat attattaaca tcctacaagg
    22441 agaacaaaag catgagtcaa attcacaaac acaccattcc tgccaacatc gcagaccgtt
    22501 gcctgataaa ccctcagcag tacgaggcga tgtatcaaca atctattgac gcaccagata
    22561 ccttctgggg cgaacaggga aaaattctcg actggatcac accgtaccag aaggtgaaaa
    22621 acacctcctt tgcccccggt aatgtgtcca ttaaatggta cgaggacggc acgctgaatc
    22681 tggcggcaaa ctgccttgac cgccatctgc aagaaaacgg cgatcgtacc gccatcatct
    22741 gggaaggcga cgacgccagc cagagcaaac atattagcta taaagagctg caccgcgacg
    22801 tctgccgctt cgccaatacc ctgctcgaac tgggcattaa aaaaggtgac gtagttgcga
    22861 tttatatgcc gatggtaccg gaagccgcgg tcgcgatgct ggcctgcgcc cgcattggcg
    22921 cggtacattc ggtgattttc ggcggcttct cgccggaagc cgttgccgga cgcattatcg
    22981 actccagttc acgactggtg atcacttccg acgaaggtgt gcgtgccggg cgtagtattc
    23041 cgctgaagaa aaacgttgat gacgcgctga aaaacccgaa cgtcaccagc gtagagcatg
    23101 tggtggtact gaagcgtact ggcggaaaaa ttgactggca ggaagggcgc gacctgtggt
    23161 ggcacgacct ggttgagcaa gccagcgatc agcaccaggc ggaagagatg aacgccgaag
    23221 atccgctgtt tattctctat acctccggtt ctaccggtaa gccaaaaggc gtgctgcaca
    23281 ctaccggcgg ttatctggtg tacgcggcgc tgacctttaa atatgtcttt gattatcatc
    23341 cgggcgacat ctactggtgc accgccgatg tgggctgggt taccggacac agttatttgc
    23401 tgtatggccc gctggcctgc ggcgcgacca cgctgatgtt tgaaggcgta ccgaactggc
    23461 cgacgcctgc ccgtatggcg caggtggtgg acaagcatca ggtcaatatt ctctataccg
    23521 cgcccacggc gattcgcgcg ctgatggcgg aaggcgataa agcgatcgaa ggcaccgacc
    23581 gttcgtcgct gcgcattctc ggttccgtgg gcgagccaat taacccggaa gcgtgggagt
    23641 ggtactggaa gaagatcggc aacgagaaat gcccggtggt cgacacctgg tggcagaccg
    23701 aaactggcgg tttcatgatc acaccactgc ctggcgctac cgagctgaaa gccggttcgg
    23761 caacacgtcc gttcttcggc gtgcaaccgg cgctggtcga taacgaaggt aacccgctga
    23821 agggggccac cgaaggcagt ctggtgatca ccgactcctg gccgggtcag gcgcgtacgc
    23881 tgtttggcga tcacgaacgt tttgagcaga cctacttctc caccttcaaa aatatgtatt
    23941 tcagcggcga cggcgcgcgt cgtgatgaag atggctatta ctggatcacc gggcgcgtgg
    24001 acgacgtgct gaacgtctcc ggtcaccgcc tggggacggc agagattgag tcggcgctgg
    24061 tggcgcatcc gaaaattgcc gaagccgctg tggtcggtat tccgcacaat attaaaggtc
    24121 aggcgatcta cgcctacgtc acgcttaatc acggggagga accgtcacca gaactgtacg
    24181 cagaagtccg caactgggtg cgtaaagaga ttggcccgct ggcgacgcca gacgtgctgc
    24241 actggaccga ctccctgcct aaaacccgct ccggcaaaat tatgcgccgt attctgcgca
    24301 aaattgcggc gggcgatacc agcaacctgg gcgatacctc gacgcttgcc gatcctggcg
    24361 tagtcgagaa gctgcttgaa gagaagcagg ccatcgcgat gccatcgtaa cccacaattg
    24421 tcggatgcgc tccgacctac atctggcaac ggctgtaggt cggataagat gcgccagcat
    24481 cgcatccgac aacaagcgcc ggatgcggcg tgaatgcctt atccggccta cattcggcaa
    24541 gggttaccca agcgttaacc ttctcccata agggagcggg aattaaaaca atccctacat
    24601 tacctctgga gaatctgtga tgaatggtac gatttatcag cggatagaag acaatgcgca
    24661 tttcagggag ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga ttattatgct
    24721 ggcagtttat atcggcttta ttttactgat cgccttcgcg cccggctggc tgggcacccc
    24781 gctgaatccg aacaccagcg tcacacgcgg tattccagtc ggtgtcgggg taattgtgat
    24841 ctcctttgtt ctcactggta tctacatctg gcgggcgaac ggcgaattcg accgtcttaa
    24901 taatgaagtc ctgcatgagg tacaagcatc atgaaaagag ttctgacggc gcttgccgcc
    24961 acactccctt tcgcagctaa cgccgcggat gctattagcg gggccgtaga gcgccagcca
    25021 acgaactggc aggcgattat tatgttcctg attttcgtcg tgtttacgct cggcattacc
    25081 tactgggcgt caaaacgcgt acgttcgcgt aatgactact acaccgcagg cggcaatatc
    25141 accggttttc agaacgggct ggcgattgcc ggggactata tgtccgccgc ctcattcttg
    25201 gggatctccg cgctggtgtt tacctccggc tatgacggct taatttactc gctgggcttc
    25261 ctggtgggct ggccgatcat tctgttcctg attgccgaac gtctgcgtaa cctcggtcgc
    25321 tacacctttg ccgatgtggc ctcttaccgt ctgaaacaag ggccgattcg tattctttcg
    25381 gcctgtggtt ctctggtggt ggtggcgctt taccttatcg cccagatggt gggcgcaggt
    25441 aaactgatcg agctgctgtt tggccttaac tatcacattg cggtagtgct ggtcggcgtg
    25501 ctgatgatga tgtacgtcct gttcggcggc atgctggcga ccacctgggt acaaattatt
    25561 aaagccgtgc tgttgctgtt cggtgccagc tttatggcct ttatggtgat gaaacacgtc
    25621 ggctttagct tcaacaatct gttcagcgaa gcgatggcgg tacacccgaa aggtgtcgac
    25681 atcatgaagc cgggcgggct ggtgaaagat cctatctccg cgctctcttt ggggctagga
    25741 ctgatgtttg gtacggcggg cttgccgcat attctgatgc gcttctttac ggtcagcgat
    25801 gcccgcgaag cacgtaagag cgtgttctac gccaccggat ttatgggcta cttctacatc
    25861 ctgaccttta ttatcggctt tggcgcgatc atgctggtcg gtgcgaatcc ggaatataaa
    25921 gacgcggcgg gccatctgat tggtggtaac aacatggcag cagttcacct ggccaatgcg
    25981 gtgggcggca acctgttcct cggctttatt tcagcggttg ctttcgccac cattcttgcg
    26041 gtggttgcgg gtctgacgct ggcgggcgca tcggcggtgt ctcacgacct gtacgctaac
    26101 gtcttcaaaa aaggtgcgac cgaacgtgaa gagctgcggg tatcgaaaat caccgtactg
    26161 atcctcggtg tgattgccat tatcctcggc gtgctgtttg agaatcagaa catcgccttt
    26221 atggtggggc tggcgtttgc catcgcggcg agctgtaact tcccgatcat tctgctttcc
    26281 atgtactggt cgaaactgac tacccgtggc gcaatgctgg gcggctggtt aggactgatt
    26341 actgcggtgg tgctgatgat cctcggcccg acgatttggg tacagatcct cggtcacgaa
    26401 aaagccattt tcccgtatga atacccggcg ctgttctcta tcagcgtggc attcctcggt
    26461 atctggttct tctcggcaac ggataattca gcggaaggtg cgcgcgagcg tgaactgttc
    26521 cgtgcgcagt ttatccgctc ccagactggc tttggcgttg agcagggccg cgcgcattaa
    26581 tctctcccct tccccggtcg cttgatcggg gaatttcttc agaacatcat cctggccacc
    26641 agcggcgaaa ccaataccat caatacacct gaaagcatca tgaccaggct tgcaactacg
    26701 ccttcttgtt gacccagttc atacgaccgc gcagtcccgg caccatgaga tgccgcgccg
    26761 aatccagcac ctttcgccat cccttcgcga atagagagac gtaaaaacaa cacgtcgcca
    26821 atcgccatgc caaacacacc ggtgaccacc acaaacagcg ccaccagatc aggctgtccg
    26881 ccgagcgact ggctcgctgc caaagcaaat ggcgtagtga ccgaacgcac cgccaggcta
    26941 cgctggatct catctggcag ggtaaacaat cgcgccagcc acactgagct ggtcactgcc
    27001 accaccgttg ccgtcagtac gcctgccgtc aacgacatcc agtgacgctt aatgatcgcc
    27061 cgattgtcat acactggtac cgcaaaggcg atggtggccg gacctaatag ccatagtaac
    27121 cagtgcgctt cgccaatata gttttgccag gaaatattgc caaaaaccag cagcataacc
    27181 agtagcgtag gcgtcagcac cagaggcatc aatggcaatt tacgaaagcg gcgataaagg
    27241 cgtttgttaa gaaaatagat aactagcgta attgccagac agagcatact tagctgaaaa
    27301 ttagccacgg tttaacctgc gcatttcata gcgatagact ttttccacta cccaggcagt
    27361 cgtgcccagc accatcaccg tactcagcgc gatcacagca aaaatacgcc agccatccac
    27421 cagtaatagt tgtgcatagt tcaccacagc caccacggca gggacaaaaa acagcaacat
    27481 ttcggccaat agccaacgcg ccccggctcg aacccattgc agaggaagaa tgcgacaaac
    27541 aatcagcgcc agcatcagga tcatccccac caggttggca ggcagcggca aatgcagcca
    27601 ggagacgaga tactgcgcaa aaataaataa acctgcatac agcacaacct gaaaaaatac
    27661 ctggatagat tgcactacgg caggggtaat gcggcgaagc gccacgggca tgagaggata
    27721 tcctgaaaat aaaacagaat ggtaagtata gagagcgctc gcccctgact gaaatgaatt
    27781 aaaatcattg caggtatagt caaagggaat agttatggat atcagaacgc tgcgttattt
    27841 tgtcgaagtc gtgcgccagc aaagcttcac ccgcgcagcg gagaagttgt ttgtcaccca
    27901 accaactatc agcaagatgc tgaagaatct cgaagatgag ctgaactgta tattgttgat
    27961 ccgcgatggt cgtcggctgc ttctcactga tactggtcgc gtcgtatttg agcgcgggct
    28021 ggctttactg gctgagtttc ggcaactgga agctgagctt agcgatatca atcagcttaa
    28081 aaccggcgtt ttgcggctgg gaatcccgcc gatggttggg atgttgatgg ccgggccgat
    28141 tggccttttt cgccagcgtt accccggcgt agaattgaaa atttcggagt ttggtggtct
    28201 gacggtacaa caagcggtga tgaacggtga actggatatg gcactgacag ccctgccagt
    28261 agaagaaagc ggcctgacaa cgttatcgct gtttagtcat ccactatgtg tactgacgcc
    28321 acattcaggt aaatggcttg aatgtgatgc cgtctcaccg gagacattgg cggaacatcc
    28381 tctacttatc tataacgaag attttgcctt aagtaagcaa ttgatgaagt tattcgatga
    28441 acatggcgtt aagccgagaa ttgcagttcg cagcgggcaa tgggattttc tcgccgcgat
    28501 ggttcaggcc ggtgtcggaa tagccatatt gccggaaccc atttgccagc gattagataa
    28561 aaacacctta aaatggctac cgctggaaag cgaactaagc tggaaattag gaatgatttg
    28621 gcgtgagggc gtttaccttt cccactctgc ccacgcgtgg ctgagctgtt gtgaaggata
    28681 ctggttaacg ccagagtaag tcgggttggc gggctcgccc gccaaccatg cagattactg
    28741 attttcctca atcagcaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt
    28801 ttcgttactg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa
    28861 tgccgccaga cggaagcgac gctccaggtt ctcttccagg attgaacttt ctacatcatt
    28921 acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactaacct ctgtgagcag
    28981 ctggttatcg atattctctt cggtatccgc cgccaaacgc tcctccattt tctggatcgc
    29041 cacaatcgcc acttctgccg ttgccgctcg tgcaatacgt tcctctttca attgttgcga
    29101 atggtccgcg acttctatgt gttgtagcag aataggcaac atcaccacgc cgacaaacaa
    29161 cgagaagaga atgacgccag ccgccaggaa caccaactca tagcgcgcag ggaagacgtt
    29221 accatccggc aagagaagcg ggatagagag cacaccggcc agagtgatcg ccccacgcac
    29281 cccggcgaaa gacgcgatca ggatttctcg tgtagtccac gaaccaaact ccatcggctt
    29341 cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag
    29401 catcagcgcc gcatatatca gaataatatc ggtaaacagc atccagattt cgacgttagg
    29461 atcgatttct gccgccatca gcgacgtctc cagaatcccc ggcaactgca gacctaacag
    29521 caggaacacc atgccgttaa agacaaattc cagcatcgcc caggtgctgt ttgcgcgcag
    29581 gcgcattgcc agcggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc
    29641 aaccgcagcg aggatgccgg aaacgccaat atgttccgca atcagataag aagcgaatgg
    29701 cagcaagaac agcagaacga tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa
    29761 tcgcagcgag cgaccataca gccagctcac cacaaaaccg gcgagaatac cgcctattgc
    29821 cactttcata aattcgaccg tcgcaccgcc gacggtgaag atcattgtcc ccatcgccac
    29881 tgccacggca aacttcaacg acaccaggcc ggaggcgtca ttcatcaacg cttcgccctg
    29941 caaaatgccc atgatttttt tcgggatgcg cccctcgcca acaatcccgg agagcgctac
    30001 agcatcggtc ggagaaagca ccgcagccag cgcaaaggcg gggatcagcg gaatgcccgg
    30061 caccacccag taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc
    30121 gaggccgaaa atctctcgcc catgttcgag aaattcacgg gtcggcgttt tccaaccatc
    30181 agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg
    30241 caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg
    30301 gatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat
    30361 cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga
    30421 aaggcgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg
    30481 gagaaaataa aaacgggcag ccattgccac ccgttttcag aatcctatgt aagattagat
    30541 agcccagcca cccgcataga aggtcaccag cgccacggca ataacgaccg taccgatgtt
    30601 caacttgcgc cattcgccgg aaaccagacg accaatcacc agagtcgcga agccaatcat
    30661 gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat
    30721 cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca gcaggccaac
    30781 gtacatcagc gccggagccg ttgcgtaccc cggaacgagg taagagagcg gagagaggaa
    30841 cagaatcagc aggaacagca cgccaacggt gatagccgtc aaaccggttt taccgcccgc
    30901 cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga
    30961 aaaaacgctg ctcatggagt cggtggtcag tgctttcccg ccgtcgatga tctgcccatc
    31021 tttatccagc aggttcgcct gaccggcgac ggcacggata gttccggtgg catcaaatac
    31081 tgccgtcatc accagtgcca gaacacttgg caggactaca ggattcagcg cgcccataat
    31141 gtccaggctg cctatcagtg aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac
    31201 gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt
    31261 cagcagaatg ccgccaggga ctttcagttt ttccaggccg ataatcactg ccagccccac
    31321 caacgacatc atcaccggaa aggtcgtaaa atcccccagc gcgacgggca gaccatcaag
    31381 cgggttttta atcaccagac cgacaccatt agcggcaatg agcagcagga acagaccgat
    31441 accaatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat
    31501 acccgtggca gaaattaccg taaacagcac acccatcagg aacacggcac ccagcgcgac
    31561 aggtacgcta atatgttgcc ccagcaccag actgaatgcg gtaaacgccg tcagagagat
    31621 ggcgcaacca atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc
    31681 ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcccgctt tacccaacat
    31741 gcctggaacg acgataaccg agtagaccat cgccagaaac gttgttaacc cggcaaccac
    31801 ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc
    31861 ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag
    31921 atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcgaca
    31981 atttgctgcg tcgcaaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    32041 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
    32101 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
    32161 tgcgcaagat tgcagcgtta tcgagccgta taccgttcag gattgcgata aaaaatcgtc
    32221 tgagatgaca aaaatattgc ggctaaaaag tgtactttta cactggcgaa gaaacgccgg
    32281 taacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
    32341 tgttgacgtc gggggaaacc ctcctgtgta ccagcgggat agagagaaag acaaagaccg
    32401 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt tcatcttcca gtaagcgtgc
    32461 gccggtacct tcttctccta agcggtcgcc cgggttacgc aacgggcaat cactgcgcga
    32521 aaggcagcca caaccaatac atccatccag ttcgtcacgc agcgccacta aggtatgaat
    32581 gcgccgatcc aactcttctc gccattggga cgaaagctgt ttccactctt tcgcacttaa
    32641 cgtatgccct tctggcaaca cgccaaacgc ttcacgaatg gttgcgagcg gaatgccaat
    32701 acgctgagca attttgataa ttgcgacata tcgcaacaca tcacgtttat atcgccgctg
    32761 attgccgcta ttacggatac tggtaatcaa ccctttgctt tcatagaaat gcagcgccga
    32821 taccgccaca ccgctgcgtt tcgccacttc gccgggtgtt agcagcgctt taatgcgggg
    32881 taatttcttt tccataaatc gctttacctc aagttaactt gaggaattat actccccaac
    32941 agatgaatta acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat
    33001 tcaggatctt atcgcatgga ttgacgagca tattgaccag ccgcttaaca ttgatgtcgt
    33061 cgcaaaaaaa tctggctatt caaagtggta cttgcaacgg atgttccgca cggtgacgca
    33121 tcagacgctt ggcgattaca ttcgccagcg ccgtctgtta ctggccgccg ttgagttgcg
    33181 caccaccgag cgtccgattt ttgatatcgc aatggacctg ggttatgtct ctcagcagac
    33241 cttctcccgc gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct
    33301 gtagttttat tgcccgcgcg ttaattcccg cgcgggtaat tgctccatcc attgcataaa
    33361 cacctgcggc ggcatcgcct tcgcgaagaa ccatccctgg caatagcgca cgccgcgttt
    33421 gcgcagccag ttaacctgcg cctcagtttc gacaccttca gcaatcgttt ttaaccccag
    33481 gctgtgcgcc agctcgatga tgtgttccgc aatcaaatga ctggttttgt gggtggtcag
    33541 cttctcaaca aacgatttat cgattttcaa aatatcgaca ttcaatgatt taaggttatg
    33601 caagttagag tagccaatac caaaatcatc aattgccact tcgtaaccgg ccttgcggaa
    33661 agccagaata atcggcgtca ttttgtcgac atcaagaaat gcatgttcag tcacttcaaa
    33721 tttaatttgc tgcgggcgca ccgcatgttg ctctgttttc tgattggttc gcgctatcaa
    33781 ccgtgaggta tgaaaatcgg aggctgacag gttaatagaa acatagcgat cggcatgtgt
    33841 tgccagatac gcgcccaaat cgcggaagac attatcaata acataatcag ttacctgttc
    33901 gatcatcccc tccttttctg ccagcggaat aaactctgcc ggattcatta cttgcccctg
    33961 ctcaccgggc caacgtaaca acgcttcagc gccgatacat ttttctgttt tgatatcgat
    34021 tattggctgg taataaagga aaagttgatg tttttcgagg gcgcgttgca atttgcgttt
    34081 gggagacaaa tagttttgtc gaatacgtag ccagagtaat agcagaacaa gactccccaa
    34141 aataccggcg ggcaacgcaa aaataagatg attataaaaa tgggttataa gacgttgata
    34201 tgaagtcgca acaatggccg caattgggcg ttttgtcgaa taaactgtcg catataaata
    34261 gccatttcgt tgtacggtta aatcattcaa acgaatcagc ggagaaaacg ttgctgccga
    34321 ggcctcgttg cttaacgaga aaaaggtttt cgtcaccgta tcatacacac cccattgcaa
    34381 tgtcggatca tcagacatca cttcactcca gaagagaggg ttaatgaccg ctacataatt
    34441 cccccgctgc atataggtca ttttatagcc tgagaaaaaa ggcgtatcac ggtaataata
    34501 gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc catttacaga
    34561 cgctatcagc gatgagcata aaaaatggtt atcacgagca taaatcaatt cattaatata
    34621 aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc aaacttgccc
    34681 ctgatagcgt tcagctgcat cgctcactaa atctgcctga cgaatgacca gctcagattt
    34741 atccaacgcc agctgagcaa aagttcggag ctgattattc acttctgatt ttgcccaaaa
    34801 gaaggcaatc catagcgaca gaatgatggg aaagagaact aaaaagataa tgcccggaaa
    34861 cgccagtaat tggtgtcgtg cacgatgact cattgctatc aattccctgc ctgtttatac
    34921 ccggttattg caaggtgaaa ccctggctac cgacgtcttt agataaatct tacggtataa
    34981 atagcacttt tgcctgatat aaaattgaga cctttttaat cgtcaccagg atgacgataa
    35041 ttcattgatc ttaaaaatta atcaccttca ggaatgcaga ggcggcggga agatataccc
    35101 ttaatgccgc tgaaaaaaca cataatctgt acggcaataa aagtcacggc ctaatctggc
    35161 taacaaacaa ccaattgtga tatagttcac aaaattcatg aaacaaacag agtgtttcat
    35221 ttttgtgttc tgcaagcccc gcgatagcca gagtgtatca cgcctcccgt gaacaacgcc
    35281 gcgctgtcca gggcatcagc tctttttcga ggaattgttt gatggctacc ctcaccactg
    35341 gcgtggttct tcttcgctgg caacttctta gtgccgtaat gatgtttctg gccagcacgc
    35401 tcaacatccg ttttcgtcgg gctgattatg tcgggcttgc agtgatcagc agcggtttgg
    35461 gcgtagtttc cgcctgctgg ttcgcaatgg ggttgcttgg catcacaatg gcggatatca
    35521 ccgccatctg gcacaacatc gagtcggtga tgatagaaga gatgaatcag acaccgccac
    35581 aatggccaat gattttgact tgatatgtag aagcctccat aacggaggct tcttttttat
    35641 ggctggcaga tgctttaatc atccacctta aaacaatata ccctattgtt ttaaagacaa
    35701 atcagaacgg aatatcgtca tcaaagtcca tcggcggctc gttagacggc gctgccggag
    35761 cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc tgcggctgct
    35821 gaggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct gcaggagcgc
    35881 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
    35941 tggtatagcg atcctgaccg gattgatcgg tccatttgcg ggtacgcagc tgaccttcga
    36001 tataaacctg agaaccttta cgcagatatt cgctggctac ttctgccagt ttgccgaaca
    36061 gcacaacgcg gtgccattca gtctgctctt tcatctcgcc ggtggcttta tcacgccagg
    36121 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
    36181 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
    36241 tcgtgtctcc tgaaaaaaaa tcgttctgaa taagtgtaaa cgcgcgattg taccattacc
    36301 aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa caaacctggc cagacattgt
    36361 tacacaacac tccgggtaat gcattccaat actgtatatt cattcaggtt aatttgtgtc
    36421 ataattaacc gtttgtgatc gccggtagca ccatgccacc gggcaaaaaa gcgtttaatc
    36481 cgggaaaggt gaatggataa gatcgaagtt cggggcgccc gcacccataa tctcaaaaac
    36541 atcaacctcg ttatcccccg cgacaagctc attgtcgtga ccgggctttc gggttctggc
    36601 aaatcctcgc tcgctttcga caccttatat gccgaagggc agcgccgtta cgttgaatcc
    36661 ctttccgcct acgcgcggca gtttctgtca ctgatggaga agccggacgt cgaccatatt
    36721 gaggggcttt ctcctgccat ctcaattgag cagaaatcga cgtctcataa cccgcgctct
    36781 acggtgggga caatcaccga aatccacgac tatttgcgtc tgctgtacgc tcgcgttggt
    36841 gagccgcgct gcccggacca cgatgtaccg ctggcggcgc aaaccgtcag tcagatggtg
    36901 gataacgtgt tgtcccagcc ggacggcaag cgtctgatgc tgctcgcgcc aatcattaaa
    36961 gagcgcaaag gcgaacacac caaaacgctg gagaacctgg caagccaggg ttacatccgt
    37021 gctcgtattg atggcgaagt ctgcgatctt tccgatccgc cgaaactgga actgcaaaag
    37081 aaacatacca ttgaagtggt ggttgatcgc ttcaaagtgc gtgacgatct tacccaacgt
    37141 cttgccgagt cgtttgaaac cgcgctggag ctttccggtg gtaccgcggt agtggcggat
    37201 atggacgacc cgaaagcgga agagctgctg ttctccgcca actttgcctg cccgatttgc
    37261 ggttacagta tgcgcgagct ggaacctcgc ctgttttcat ttaacaaccc ggcgggagcc
    37321 tgcccgacct gtgacggact tggcgtacag caatatttcg atcctgaccg cgtgatccag
    37381 aatccggaat tatcgctggc tggcggtgcg atccgtggct gggatcgccg caacttctat
    37441 tatttccaga tgctgaaatc gctggcagat cactataagt tcgacgtcga agcgccgtgg
    37501 ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg gttctggcaa agaaaatatt
    37561 gaattcaaat acatgaacga tcgtggcgat acctccattc gtcgtcatcc gttcgaaggc
    37621 gtgctgcaca atatggagcg ccgttataaa gagacggaat ccagcgcggt acgtgaagaa
    37681 ttagccaagt ttatcagcaa tcgtccgtgc gccagctgcg aaggaacccg tctgcgtcgg
    37741 gaagcacgcc acgtgtatgt cgagaatacg ccgctgcccg ccatctccga catgagcatc
    37801 ggtcatgcga tggaattctt caacaatctc aaactcgcag gtcagcgggc gaagattgca
    37861 gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc tcgttaacgt cggcctgaat
    37921 tacctgacac tttcccgctc agcagagaca ctttccggcg gtgaagccca gcgtattcgt
    37981 ctggcgagcc agattggtgc aggcctggtt ggcgtgatgt acgtgctgga tgagccgtct
    38041 atcggcctgc accagcgcga taacgagcgc ctgctgggta cgcttatcca tctgcgcgat
    38101 ctcggtaata ccgtgattgt ggtggagcac gacgaagacg cgattcgcgc cgctgatcat
    38161 gtgatcgata tcggtccggg tgcgggtgtt cacggcggtg aagtcgtcgc ggaaggtccg
    38221 ctggaagcaa taatggcggt gcctgagtcg ttgaccgggc agtacatgag cggtaaacgc
    38281 aagattgagg tgccgaagaa acgcgttccg gcaaatccgg aaaaagtgct gaaactgacg
    38341 ggcgcacgcg gcaacaacct gaaagacgtg acgctgacgc tgccggtggg tctgtttacc
    38401 tgcattaccg gagtttcagg ttccggtaaa tcaacgctga ttaacgacac actgttccca
    38461 attgcccaac gccagttgaa tggtgcgacc atcgccgaac cagcaccgta tcgcgatatt
    38521 caggggctgg agcatttcga taaagtgatc gatatcgacc aaagcccaat tggtcgtact
    38581 ccgcgttcta acccggcgac ctataccggc gtgtttacgc ctgtgcgcga actgtttgcg
    38641 ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac gtttcagctt taacgttcgc
    38701 ggcggacgct gcgaagcctg tcagggcgac ggcgtgatca aagtggagat gcacttcctg
    38761 ccggacattt acgtaccgtg cgaccagtgc aaaggcaaac gctataaccg tgaaacgctg
    38821 gagattaagt acaaaggcaa aaccatccac gaagtgctgg atatgaccat cgaagaggcg
    38881 cgtgagttct ttgatgcggt gccagctctg gcacgtaagc tgcaaacatt gatggacgtt
    38941 ggtctgacgt atattcgtct gggtcagtcc gcaaccacac tttctggtgg tgaagcccag
    39001 cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg ggcagacgct gtatattctc
    39061 gacgagccga ccaccggtct gcacttcgcc gatattcagc aactgctcga cgtactgcat
    39121 aaactgcgcg atcagggcaa taccattgtg gtaattgagc acaatctcga cgtgatcaaa
    39181 accgctgact ggattgtcga cctgggacca gaaggcggca gtgggggcgg cgaaatcctc
    39241 gtctccggta cgccagaaac cgtcgcagag tgcgaagctt cgcatacggc gcgcttcctc
    39301 aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc cttcttcttt cagagttgca
    39361 ccagcaattt acgtttttct tccggcagca aattcaccgc ctgctgataa gacgcatcca
    39421 ccagataata gatttgcgaa tccggcagcg atccgtcgag atacacggtg ctccagtgtg
    39481 ctttgttcag atggcggctt ggacgcacat cgctgtgctg ctgacgtagc agctccgcca
    39541 gctccgggct ggttttcagc gaaacagctg ggcgattttc tacttctttc accatcgcaa
    39601 acagcacatc ttccactttg atctgcgtcg ctttccagtc gttatgcacg ctctgttctg
    39661 cgcctggttt tgccatgcaa tattgtagca actccaaaat ggtcattttt tactcccctt
    39721 gaagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg atgttccccc agccagatgc
    39781 cttgccaggt gccggtctga atacgccctt tatgcaccgg caatacaagc gatgttccca
    39841 gcattgagga tttgatatga gaaggcatat cgtctgctcc ctcatagtca tgctcataat
    39901 ttccgttgtc gggaacggcg cggaggaaaa aacgctccat gtcgtggcgt acggtgggat
    39961 cgcagttctc attaagtgtc agagaggcgg aggtatgttg cagcaacaga tgcagtaaac
    40021 cgatgttaac gcgcggcata tcagccagct gattcagaat ttcatccgtt accagatgaa
    40081 acccacgaga tttggcgcta agcgtgagcg tcttttgata ccacatgtgc tgctccttga
    40141 taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg ataaaggtgt gattaaaaaa
    40201 acagcattcg ggagagtatt acgctctccc gctctgtcag tattctgaat tgacgatcac
    40261 ctcttcacca aacgcacccg cttgtggcaa gggtttgtag gtagagttgg aggcgcgcag
    40321 aatgcggata ccacgagcgc caacatcgcg tgcggcggta atatcgttat cggaatcacc
    40381 ataaaaaatt cggatatttt tatcctgcag ccattgcgat tttgtatttt gacctggttt
    40441 atcgcccgca aagatcaccg gattcatgtt ggtggcagga atatgaaaat tatccgccag
    40501 cgtttttgaa accgtttctg ttttggtcgg gctacgacca gtcacaaaga agatcgcgtc
    40561 accgcggcgt acatgcatat caatcagctg gcgagcgacc tcttttggaa tgctgaattc
    40621 atcccagcca ttgttcattt tttcccagaa cacaggattt ttcagataat cttcgctttc
    40681 tggtgagaag gtttttttgc cgcgccagaa acccggactg gaaaaaagta ccgtatcatc
    40741 gatatcaaac cccaccgcca ttggcggacg tcctgcgagg ctattttcaa tttgtgcaac
    40801 cgaaacccaa tgaattggtg cctgttcagc gagtttggct acgttagtac ccggattaag
    40861 cggtgaagga gatgaggcca gggcaacagc ggaactgttt agagcgaaca ataagcaaac
    40921 ggcactcaat gcctgtgtga tcttgcgcat atttttccct aaatagtcag taagttgaaa
    40981 cttattattg tgcgtttgtt gaaaaaacca tctgaccata acgccaaccg caggggaaag
    41041 gaaggattat ctgcggattt gtcacataaa aagagcatga tcacatttgg aatacctgtg
    41101 cgataaacca cacattgcca cggttattct gcttacacat tcacacaatg gcactctttt
    41161 ggttctcgca aacattgttc agggaatata ctgatgataa atataaatgc attcaatgga
    41221 tgatgaatga aaatatcgtg gaattatata tttaagaaca aatggcgatt tcacattaca
    41281 agcatttcac tttttcttat catgctcgcg gtttcaatcg cttttttgca cttgcgtttt
    41341 aataccttgt ccggtaccga taaaatgcgg cttgaaatgt ataagtccac attatattcc
    41401 accatcgagc aattttatgt tttaccctat atgctctcaa cagaccatat catccgtcag
    41461 gcggtaatta cgcctgacga tatgacgtcc agcgaactca atcaacgaat tgcacatttc
    41521 aatactcaac tcaaaaccgc agcaatattt attctggata cccaaggtaa ggccatcgct
    41581 tctagtaact ggcaggaccc cggcagctat gtagggcaaa attatagcta tcgcccctat
    41641 tataaacacg ccatgtctgg cttaaatgga cgcttttacg gtattggtag cactacgaat
    41701 acaccgggat tcttcctctc tacaagtata aaagataaag gaaaaattgt cggtgttgta
    41761 gtagtaaaaa taagtcttaa tgaaattgaa aaagcatggg ccgaaggtcc tgaaaatatt
    41821 atcgtgaatg atgaacatgg gattatattt ttaagttcaa aatcgccatg gcgaatgcga
    41881 acactgcaac cgttacctgt tcaggcaaaa caaaaactac aatctacccg ccaatatagt
    41941 ctcgacaatc ttttaccggc ggattattat ccctgttata ccgtgagcaa ttttactttc
    42001 ctgaaagata aaaaagaaca actctgttta ttcccgcaat attatacgca acaaatagcc
    42061 attccagaat ttaactggaa aatgacaatt atggtcccct tagataacct gtactggtca
    42121 tgggctattt cgttagtcat tacactaatt atttacctgc tgtttttgtt atttattaaa
    42181 tactggagaa tgcgatctca tgcacaacaa ttattaacac tggcgaatga aacattagaa
    42241 aaacaggtta aagagcgtac atctgccctg gaattgatca atcaaaaatt aatacaggag
    42301 ataaaagagc gcagtcaggc tgaacaagta ttacaaatta cgcgtagtga actggcagag
    42361 tccagcaaac tggcggcgct tggacagatg gcaaccgaaa ttgcccatga acaaaatcaa
    42421 ccgttagccg ccattcacgc acttactgat aacgcgcgta ctatgctaaa aaaagagatg
    42481 tatccacagg ttgaacagaa tctgaaacac attatttccg tgattgagcg gatgacgcag
    42541 ctcatttccg aacttaaagc atttgcctcg cgccatcgcg tacctaaagg ttctgccgat
    42601 gtcatcaaag tgatgtatag cgccgtggcg ttacttaatc acagcatgga gaaaaataac
    42661 attgagcgac gaataaaagc cccatccatg ccgttatttg tcaattgcga tgaactcggt
    42721 cttgaacaga tattcagtaa tttaattagc aacgccttag attctatgga aggtagctct
    42781 tacaaacgac tggatatcgc cattcgccag gcaaataaca aagttattat taccattaaa
    42841 gacagcggtg gcggttttgc acctgaagtt gtcgatcgca tatttgaacc attttttacc
    42901 actaaacgta gaggaatggg gttgggactg gcaatagtca gcgaaattgt ccgaaattcg
    42961 aacggcgcac tccacgccag taatcatcct gaaggcggcg cagtaatgac attaacctgg
    43021 cctgaatggg gagaaaaaca tgaataataa attcagtgaa attactatag tttatattga
    43081 ggatagtgac gatgtacgtt ttgcctgtga acaaacgctc acgctggctg gttatcgcgt
    43141 tatttcctgt tgcgatgcag aacacagtat tccccttatt caaagtcagg ccaacattat
    43201 tatactgacc gatgtcaggt tacctggaat atcaggacta gagctcctca gttacatcaa
    43261 cgaaatggat agcaaaattc cggttatttt aattaccgga cacggtgacg tggaaatggc
    43321 ggtggatgcc atgcgtaatg gtgcattcga tttcattgag aaaccaagta gctcagataa
    43381 attattgagt attattgcca gagcggtaga aaaacggcga ttggttttag aaaatcagca
    43441 attactggcg aatctgcaac aggaaaatgg cccggtgctg attggtcgta gtccgcagat
    43501 gcagcaactg cgtaaaatga tccttaatgt cgcagatacc ggtgccgatg tccttattta
    43561 tggtgaaaca ggttgtggta aagaagtcgt cgccagaatg ctgcatcact ggagtacccg
    43621 ccgtcagggg cagtttgttg ccttaaactg tgcaggctta ccagaaacgt tgtttgaaag
    43681 cgaaatattt ggtcatgagg cgggcgcatt taccggtgcg gttaaaaagc gtattggaaa
    43741 aattgaacat gcaaatggtg gtacgttatt tctcgatgaa atagagggaa tgccatcagg
    43801 aatgcaggta aaactgctac gtgtattgca ggaaagaacc attgaacgat taggtgccaa
    43861 tcaattaatt ccggtgaatt gccgtgttat tgcagcgacc aaggaagatt tactgcgtcg
    43921 cagcgaagaa catctgtttc gtctcgatct ctattatcgt ctgaatgtgg ttagcctgaa
    43981 tattccacct ctacgccagc gacgagaaga tatccctgaa ctgttttact ggttcgccag
    44041 tcaggcggca caaaaatata atcgtcctct gcctgatatt tctcccatgt tactggcctg
    44101 gttgcagtca caaagttggc ctggcaatgt acgtgaactt aaacacaatg cagagcgttt
    44161 tgttctcggt ttactgaccc accaccagcc agtgccaatg actcagcaag aagagagtgg
    44221 tcttaccgcc tgcattgatg ctttcgagaa aaagttaatc gaagatatgc tgcgacaaac
    44281 ggaaggccag gtcagtttga cagcgcggct attacagctg ccgcgcaaga ctttatatga
    44341 caaactcaac aaacatcaga ttcaaccgca agtttatcga ccagaatcat ccagctaatg
    44401 ctgattcctg aggatgtaac cccaaatcga tacctttgcg ggcgtaatgt tcaactaccc
    44461 aggaccagaa tgataccggg atcgtaatca tcttcgcatt gcgttcccgg ttggcagctt
    44521 ctggcatacc ggggtaaatc accggattgc cgccaggttg aggctgcgta gctaacagat
    44581 aatccgcaaa gaactgccct ttctcccctg ccagatcggt gaattgatca ggatcgataa
    44641 tcaacgcaaa aagatgatta tgcgcagatc catccgtctg atgatcttcg gcgatcgtgt
    44701 caccaccaga taagatcccc gccatcattt caatcatcag tgccaggcca aatcctttat
    44761 gctcaccaaa tggcagtaac gcccctggag gattgctgaa taacacccca ggatcattgc
    44821 ttggattccc ctgtgcatca atcaattgtc ccggcgcggc ctgcttcccg gtggacgaca
    44881 ttacccgtgc tttatttagc gccatgctac tggtggcaaa atcaaccaga atcggcggac
    44941 gcccatcact gaacggccag gcaaagcaga aagggttagt ggtcatacgc ggttccgcac
    45001 cgccccacgg ggcgaccagt gcaggacgcc cggtgacgtt actcatggcc agcatcgcca
    45061 ggccttgatc agcaaccatt tgcccgtaat gccccatacg ccctaaatga tgcgcatggc
    45121 acaacgttaa caggcaaaaa ccgcggtgtt ttgtttgttc gatggcagca tttaccgcca
    45181 cttttccggc gtgttgacca aagccattgt gggcatgaaa acgctgtaaa ttaccttcac
    45241 ttgttaagca ttccgggcgg gcattcgcgg ttacatcgcc acgagcgata ttttccagat
    45301 atttcggtag cagcgtgacg ccgtggctgg caaagccata acgttccgct tcaatcatat
    45361 gttccgccac atcctgagcg atatcggcat cacagccatt aagttccagc agcccggcag
    45421 ctaaccaacg cactttttca acagcaattt gtaatgacat cgttggtttc ctcacattag
    45481 acctaaaatt ttccaccagg tggcggcaaa cagtagcaac aacaggtagc cgattaccgt
    45541 caggtacagt cccacacggg taaactggcg tggcgtgaag gtatccgtac ccatacacac
    45601 catattttgt ggcgcgttaa tcggcaggat gaagccaaaa ctgacactga aggcgagcag
    45661 aatggtcatg ccgaccgggt taacgccgag ttctggcggc agactgctta acaggctaat
    45721 caggatgggc agcaatgcgg cagttagcgc agtggcactg gcaaacccca ggtgaatgat
    45781 tatcaagaaa gcggcgagaa tcgcgaagat ggcaagtgat ggtaaaccgt caagaccaaa
    45841 acctttcacc acatagttcg ccatccatga agctgcctgt gtatcaagca acgtggaccc
    45901 caggctgata ccaataccaa acatcagcaa cgtgccccac tgcacacgtt tttcgacctc
    45961 tttccagctc attacaccga tgcctggcag caacataatc gccagtccgg caagggtgac
    46021 agaggtggta tcaatactgt gtaattttcc gccagtagac caaaagagca gtaacaacag
    46081 cgaaataccg atcaagcgtt tttctttacc ggtggttggc cctaactctg cgagtgcttt
    46141 tttaatggcc tcgctccccc cttcaacagc ttccgtttct ggcggcaaca atttacgtgc
    46201 gaggaaatag aggatcaccg acatggtgag gctccagggc gcgcccgcta acagccagtc
    46261 aagccagctg acagaatgcc cggcgccgaa agttttattg ataaaaccga tagaaagcag
    46321 gttttgcgcc gccgaagttt gaataccaac gttccagata ctggtcgcct gggcgataac
    46381 aatcatcatt gacgccgcta aacgtgaatg tttatccacc ttaaatgccg caatgacccc
    46441 catcataatc ggcaccacgc aagccgtacg ggcagtggca ctgggtacga ccaggctcag
    46501 gacgatggtc accacaatgg caccgataat aatgctgcgg ctactggcac ctattttcga
    46561 catagtaaac agcgcaatac gtttatccag cccggtaatg gtcattgccg ccgcgataaa
    46621 cattgctgct gccaccagcg ccagagcaga attagaaaaa cccgacagag tcattttcag
    46681 cgctttcgcg gtgccaagga tcgtgtccgg gtgattcata tccggggcaa aaccaaccat
    46741 aaagataatc agtgcggaaa taacaattgc actggcggtg taatccatcg cttcgctgat
    46801 ccagacaata atggcaaaca ccagaatggc gatcatattt tgtccggcga cgggcaaggt
    46861 atcgcccaga ggcagaagag taataataat tccggcaatg atcgccaacc acaggccggg
    46921 attagggata attcgacgct tttgtggtgc gccatgtgat attgatgaca tattcacctc
    46981 attgtgaaaa ataaaaaacc tgcctggcta ttgcaggagc tatgccatct ttaatttttt
    47041 aataatgctt tgttatttaa tgattttatt atttattctt tttattttgt gcgcggtttt
    47101 ccgcacagta aataaaaggt gattctctta ccacacagat aaaaaaaggg agcgatatgc
    47161 tcccttgata ttcatttatg gattacttaa taacattcca taacgtttcg ccaagcaacg
    47221 ccgggtttct tgtccatgcc atgcctgctt tatcgaacgc acggaattta tcctctgcac
    47281 tgccgctacc gccagaaata atggctccgg cgtgtcccat gcgttttcct tttggtgcag
    47341 taacaccggc aatataacct acgaccggtt tattcatatc atggcgaata aattccgcgg
    47401 cccgttcttc cgcattacca ccaatttcac cgatcataat taccgcgtct gtttgcggat
    47461 cattttcgaa taaacgcagc gcatcaataa agttagtccc cggaaccgga tcgccgccaa
    47521 taccaataca ggtggattgc ccaagtccca gcgccgtggt ttgcgctaca gcttcgtagg
    47581 ttaatgtacc ggagcgggaa acaatcccaa tacgccctgg gcggtggatt tcaccaggca
    47641 taatcccgat tttgcactca cccggcgtga taactcctgg gcagtttggc ccaatcagcc
    47701 gcacatccgg gtggcagtca agcagccgac gaactttcac catatccagt gtcggtacac
    47761 cttcagtgat caccacaacc aatttgacgc cagcttcaat agcctcaaca acagaatcca
    47821 gcacaaacgg tgcaggcaca taaatcacgc tggcatcggc ctcagtttgt cgcatcgcat
    47881 ctttcattga atcaaaaacc gggcgatcca gatgccgttg cccgccctta ccaggcgtca
    47941 ctccacccac aatctgcgtg ccatacgcaa tagcctgctc tgtatgaaat gtgccgtttt
    48001 tgccggtaat gccctgcacc agtacccgcg tgtgtttgtt gatcaaaacg ctcatcttaa
    48061 cctcctgtta attcagtaac gcaatgatgc gtttcgcggc atcatccaga gaatcgacgg
    48121 cttccaccgt cagaccactt tctgccagta accgctgccc ttcggcggcg ttattaccgg
    48181 agagacgcac cacgacaggc agggtgatgc tggcctcatt cagagcatga ataatggctc
    48241 tggcgatcat gtcgcaacgg acaatcccac caaaaatatt aaccagaatg gctttcactt
    48301 tgctgtcgga aacaatcagc cgaaaggctt cactgacgcg ctcctgggtt gcgccaccgc
    48361 caacatccag gaagtttgcg ggttgttcac cgtatagctt gatgatgtcc atggttgcca
    48421 tagccagccc tgcaccattg accatgcagc cgatatttcc gtccagcgag acataattaa
    48481 gatcaagctt cgctgcctgg ctttcgcgtg gatcttcctg ggtttcatca cgtagcacct
    48541 ggagttccgg gtgacgatac agcgcgttat catccagcga tactttcgcg tctgcacaca
    48601 taaattcgcc cgtttcccgc aacaccagag ggttaatttc cagcagggca aaatccagtt
    48661 cgttaaaggc tttccacgcc tgattaacca ggcgactaaa ggcagcaaat agtccgtgtt
    48721 ccagttgcag aacagcaaac atttcgcgga tatggcaagg ctgcacgcct gtcagtggat
    48781 caatgctgac actgctgatt ttctccggtg tctcatgggc gactttttca atttccacac
    48841 caccttccgg gctgacaata aacgtgaccc gctggctttc acgatccacc accatgccaa
    48901 aatagagttc ctggcgtacc ggataaatgt tttcgcacag taaaatactg ctgacatact
    48961 gtccttctgg cccggtctga taagtcacca gttgcgatcc caacatttgc tgtacaaaag
    49021 cctgtgcttc cggcagctgc ttaagcactt ttacgccgcc agctttaccg cgcccaccag
    49081 catgtacctg cgctttaagt accgcgcctt tactcgggca agcaatatgt tgccaggcat
    49141 ctgccagttg cgaaatctgt tggatagcta tctcttttgg acacggcatc cccatgccag
    49201 ccagtaacga tttggcctga tactcatgta aattcatata ttccctcagt ggtgtgtgtt
    49261 atccgtgtaa tgctttatta ttcaaggcca ttgctgcttc gtggatgact tcgcttaatg
    49321 tcggatgcgc atggatcgca caggcaatat cctcgccaga ggcgctaaaa gtcatcgcca
    49381 gcgcaatttc attaatcagc tccgatgcct gcgggccaac aatcgctccg ccaagtacgc
    49441 ggtcagtgtg tttatcactg tataacgtgc aacgcccacc ttcctggcct aatgctaagg
    49501 cgcgaccgtt accggcaaac agagaatttc ctttgttaaa gactctgcca gcggctttca
    49561 gtgaggcctc gttttcccct acccacgcaa cttccggttg ggtgtaaatc accgaaggga
    49621 tcagcgcaaa attaatcggc tctaccacca gtccggcaat ttgatcagcc accactacac
    49681 cttcagccat cgctttatgc gccagcatcg gccctcgcac gacatcacca attgcccaca
    49741 gcccggcttt cccggtacgg cataagttat cgacggcaat accgccacgg ttatcggctt
    49801 caagaccgag ttgcacgaga tcgacaccag aaagtcgcgg tacacgtcca atggcaagaa
    49861 ttaatttatc gaagcggctt tcttctcgct tctcaccctg ccgccagcgc acatgcacac
    49921 cgtcatcccg ttgttcaata gcctcaatct ctactgccag ttgcattttc atcccactgg
    49981 caatcattgc tttacggact tcgttcgaca ggcgagcttc cagtgcaggc aagaacgttg
    50041 gcgccatctc cagcaacgtg acgtctgaac caacccgatt ccacacagac cccagttcaa
    50101 ggccaatcac gccagcgccg atcacaccga gacgcggcgg cacttcgctc aatgccaggg
    50161 cgccacgatt gtcgaggatc tgttgattat caatggtaac gccaggcagc tgacgcggct
    50221 gagaacctgt tgcaatcact acattacggg cgtgaaggtg ttgatcgttt acacgtaatt
    50281 gccaaatctc atcctgagca cattctaacg ttgcgaggcc gcacaggtgc ttcactttat
    50341 ttttcttaaa cagcaagctg atgcccattg tcaggcggct tactatggcg tctttacgtt
    50401 gaatcatcgc agctgcattg aaagatacgc cgtcaacatt aacgccatga atgctcgcct
    50461 catgctgcac ttgcgcgtac agttcggaag attgcagcaa cgattttgac ggaatacagc
    50521 cgacattgag acacgtaccg cccggcgaag gttcgccctg tgcattaaca ccatcatcaa
    50581 tacatactac ggaaagccca ttctgcgcag cccgcagtgc cgctacgtaa ccacctggcc
    50641 ccccccccat aactgccaca tcaaaaattg tactcatcgg cgccctcctt aaagatccag
    50701 caacagttgt tccggtgatt ccagtagctc acgtattgcc accaatgtat gcacggcttc
    50761 ctgaccatcg ataatgcgat ggtcatagct gagagcaagg tacatcatgg gacgaataac
    50821 aacctgaccg ttttctgcga ccgggcgcgg cgtgatggca tgcatcccaa gaatggccga
    50881 ctgcggcggg ttaatgattg gcgttgacat catcgaacca aaagtaccgc cgttggtgat
    50941 agagaacgtg ccgccctgta acgcctccag cggcaatttg ccattgcgtg cctgtgtggc
    51001 gtattcggca atttgccgtt caatttccac caaagagagt gattgcgcat tgcgtaacac
    51061 aggcactacc aggccgcggt tgctgctcac tgcaatgccg atatcgcaat aatcacgcca
    51121 gataatctcg ttgccatcga cgctggcatt cactacaggg aagcgctcca gcgcccgggt
    51181 aacggcctta acaaagaagg acataaagcc cagcttcacg ccgtgcttct cggcaaaacg
    51241 atctttccag cgggcgcgta agtccatcac gctctgcatg tttacttcgt tgaatgtggt
    51301 aagaatggcg ttattttgtt gggaagccag cagacgctca gcaattcgct gacgtaagcg
    51361 cgacatcggc tcacgacgtt cctgacgagc acccggtgtc aagggtttag ctggcgcgat
    51421 ctccgccacc cgttcaggct gaatggctgg tgcaggagta acgcgctgca catcctcttt
    51481 caggatacgt ccatttctcc cgctccccgc aacgtcagca agttcgacac cactacgctg
    51541 tgcctccagc cgtgcggaag gcatcgccag ggtttcggtg actggtgtga cggtttcttc
    51601 tatgacagct tgcggcttaa gatgcgccaa cagttgcgcg gacgtgaccg tgctaccttc
    51661 gctgacgata atattgctta acacgccatc gtgcggggcc ggtatttcca gaatgacttt
    51721 atcggtttcc agctcggcaa tcacatcatc gcgttttacg tgctcgcctt cttgtttaca
    51781 ccaggttgtc agcgtgcctt ccgtgacaga ttccggtaat acagggacag taatttcgat
    51841 cataaaatgt tctccttatt atttttgtgc gcctttcatg gctgaatatc tgcaaacgca
    51901 gcagcaagaa attcgtcgat ttgttgttta tgtacacgcc cataccctgt cgctggtgct
    51961 gccgctgccg ggcggcctgc atactgccag taaggagtgt taattttcag tgccgccagc
    52021 tcatgacgaa tctgccgcca cgcgccctgg ttttctggct cttcctgcaa ccaaatccat
    52081 tcgcagcagt tcggccaact cgccagaaca tcattcagtt ctgcgaccgg gaacgggtag
    52141 agttgctcca ggcgaacaat cgctacctcg tcttcatgct cacgctgctt acgggcttcc
    52201 agtacgtcgt aatagacctg gccgctacac aaaataacgc gcttaacttt ctgggactga
    52261 tgcaattgcg gatcggtgat taccggttta taagcgtcat cagtgaattc acaaagctcg
    52321 ctcatcgcgc ctttaaaccg aagtaaacgc ttactcatca ttatcactaa cggtttgcgc
    52381 attgggcgta atgcctgacc gcgcaacaaa tggaacattt gtgctgactc actgggcatc
    52441 accacctgca tgttgttttc tgcgcacagt tgcagccagc gctcaggacg tgcggaagag
    52501 tgctccggcc cttgtccgtc atagccatgc ggcagaagaa tggttaaacc gctatagcga
    52561 tcccacttgg tttcgccaga cgagataaat tggtcaatag caacctgagc gccgttggca
    52621 aagtcaccaa actgtgcttc ccaaataacc agttgctgag gcgcagacgt tgaatagcca
    52681 tattcaaagg ctaagagcgc ttcttcatta agcaccgaat cataaacatc gaaagatgcc
    52741 tgaccagcgc ggatatgctg taacggcaga taacgacggg cttccgtttg atgatgtacc
    52801 actgcatggc gatgactgaa cgtgccacgc cctgagtctt caccagaaag acgcacacca
    52861 acacccgcat ccacaagcga tgcataggcc agcatttcag ccattcccca gtctactggc
    52921 tgagtacctg cggccatatc ctggcgtaac gccagttgcc gtttaatggt aggatgtgcg
    52981 accacatcgg gtggcagtgt agaaatgatt tcaccatacg ctgccagctt ttgccgaggc
    53041 aatgccgtag ataccggggc gctccagtga gggttggtca acccatacca gttagcgcta
    53101 aaagagtgaa ttgcaggttt gagaggctgc ggttcacgtt tctggcagct atccagccag
    53161 tcgcgatagc gtgccgtcat ctcatcttgt tgcgcctgag ttaacaggcc gcggcgggca
    53221 agagattcac cataacgggc gagcgtacca gggtgagcat ccactgcctg atacatttgt
    53281 ggctgggtaa gacgtggctc atcgctttcg ttgtgtccgt gcttacggaa gcagcaaata
    53341 tcgatgatga tgtcacgccg gaaggtgtct cgccattcgc acgccagttc cattacctgg
    53401 caaacagcgt caacgtcatc gccattaacg tggataattg gggccgctac cattttggca
    53461 atatcggtgc agtaccgtga agaacgcata tcctgcaagc gggaagtggt gaagccaatc
    53521 tgattgttaa tcaccagatg tagcgtaccg ccagtgccgt agccctgagt ctgcgataag
    53581 ttaaacgtgg tttggttgac gccgaggccg cccaacgcgg aatccccatg aataagtacg
    53641 cccaccactt ttgcctgacc atcctcacca cggcgttcct gcctggctcg cacctggccc
    53701 agcaccaccg gattcacgat ctccagatgt gaggggttat acgccagcgc cacatgcagc
    53761 gaaccggcag gtgtttccag attgctggaa taccccatgt ggtatttcac atcgcctgaa
    53821 ccactgccga tcgtctgctt gccttcaaac tcagcaaaaa gctgtgccgg gtctttattc
    53881 aacagattaa ccagaacatt aagtcttccc cggtgcgcca tgccaatgac catttcctcc
    53941 acaccttgcg cacgcaggcg ttttgtcagc gtatcgagcg ccgggattgc actttcgccc
    54001 ccttccagag aaaaccgttt ctgcccaaca taacgggtat gcagataacg ctctaacgtc
    54061 tctgccgcca tcagtttttc aaaacgagca atacaggttt gggcatcagc ttgtggcgca
    54121 ttgctgctct caatccgtga taacaaccag ttgatttctt cacgattttc cagatgagca
    54181 agttcataag cctggctact ggcccaggct tgctccagca ggttaagcag ttgcttaagt
    54241 ggcatttgcg tggtgtgtgc gccaaaggtg acgctaaact cctgcaacaa gtcttcttcg
    54301 cttaatcccc aaaaaccagg ctggagggag ggaacgtcag ccgggggatt caaaccaagt
    54361 ggatcaagct ttgcgcgcag atgcccctgc gtgcgccagg cattgatcag ttgtattacc
    54421 gcagcctgtt tttttaaggt cgcaccgctg atattgttat caccggatac gctttggaca
    54481 gtcagttcag gtgaaagaaa aaaacgccgc cagtcttccg ggagttgttc cggcgtacgc
    54541 aaccaggtct ggtagtactc ttcaagccag gtcgcgttat ctccactaag cagcgtgccg
    54601 ggcgaggtca tgttctccat gtttttctcc tgatggtcat agacagctgc attactgcac
    54661 aatccgtacc aacagttaaa aacactaatt ttcaaaaact tatagaaaag agatgatgta
    54721 aaagtgggcg aaaaaaggga caaaatagta gggagtggct ggagtcagga acaggcggag
    54781 agatccgcct gttgattaaa atcacatcac cgcagcaaac gcctttgcca cgcgatgcac
    54841 attttgcgtg ttaagcccgg cgacacacat gcgaccgctg gcgatgagat agacaccaaa
    54901 ttcttcacgt agttggtcaa cctgagcggc acttaaaccg gtataactga acatgccgcg
    54961 ctgattaagc agataatcga aattgcgttc tggcatctct gtgctcaata ccttcaccag
    55021 ttcctgacgc attgccagaa tgcgagttcg catctcttct acttccacca gccagctggc
    55081 tttcaatgcc tcgtcattca gcaccgcagc caccacctgc gcaccaaaat tcggcgggct
    55141 ggagtagttg cggcgaacag ttgctttcaa ttgccccagt acgcggcctg cggcttcggc
    55201 atcttcacac agaacagaaa gtccgccgac gcgctcgccg taaagggaga aaattttcga
    55261 gaacgaattg ctcaccagag cgggtaatcc agcgctggca atggcgcgaa tggcgtaggc
    55321 atcctcttcc ataccggcac caaatccttg ataggcaata tcaagaaatg ggataagctc
    55381 acgggctttg agaatttcaa tcaccgcatc ccactgctca ttagtgagat cggcacccgt
    55441 tgggttgtgg caacatggat gcagcaacac aatactgcgg gcaggtaatg ttttcagcgt
    55501 cgccaacagg tcattaaagc gcacgccgtt agtcgcttcg tcataccagg ggtaagtact
    55561 tacttcgaat ccagccccgg cgaatattgc tacgtggttt tcccaggtag gatcgctgac
    55621 ccagacgctt gattccggga agtagcgttt caggaaatct gcgcccactt tcaatgcccc
    55681 tgagccgcca agggtttgaa tggttgctac gcgctgttgt tgcagtaccg gatggtccgc
    55741 accaaacagc agcggcgcaa tggcatggcg atagctgtta agcccttcca tcggtaaata
    55801 aagcgaagca ccatgaggct gcgcattcag gcgcgcttcc gcatccgcca cggctttcag
    55861 ttgtggaata attccgtctt cgttgtagta cagaccgata cttaaattca ctttgtcgct
    55921 gcgagggtct tctttaaaac gctccataag cgtaagaatc gggtcgccag cgtaggcgtc
    55981 aactttttga aacacgcgat ggttctccag gtttacgggc aggtggttaa accacaataa
    56041 accggaagaa ggcgaagatc gagaggatgt tcaaagtcag tcgggaagtt ggcgaagaat
    56101 ttgctccact cgctcttgca gtggccccga aaccgaaaga taaggaatgt tcctgcgcga
    56161 caattcctgt tcataccaca cctgttgccg tgcgcgaaat acctctcctt gccgggtgcc
    56221 atcctgcacg aagggaaatt ctgcgccgca aagcactacc agagagtatt cccgctccgc
    56281 cagttggtga agttcttgcg gagcatgacc atattgatca agggcataga ataaggtcgt
    56341 gagtggcgac gtgtcacata taagatagtg attagcttct gcttgttgtt cccggcgtac
    56401 ctgttcgcag gcgatatgca acaaatcatc cgcagttaag ataccgtttt tttcttccca
    56461 atagtcgcgg ccaaattcag cgacatacac cgtatcaagc acgcccgcca gcgcttttga
    56521 aagcgtgctt ttaccggtag attcgccacc aagcagacag atacggcgaa cgaaactgta
    56581 ataaacatca ttcgccaaca tgtagcgata gcggtgaaca tcggaacgaa taagcgttcc
    56641 cgaaggcgct tcatcgccaa ctgggcgcgc cattcgaacg tgttcaacgg gttgggcaaa
    56701 acgccgcgcc agcacgttag caaagccatc gccatagtct tctgcagtaa atacagcgtg
    56761 tgggcggcaa cgtaatatct gcaagcatag cgtcgccaca taatgacggt ggatgtcggc
    56821 atcagcattg ttatgcggga tagcaggtaa gttataacgc gcaacaagct ctggcgtcag
    56881 taccaaaaac gtcgcttgcg ggaagcgcac ttgcagccac gtcaggcgct tttcaggttc
    56941 acagtcaggc atttccggca cggaataact gatgatgaac agttcttcac attgcgccag
    57001 tgcggtattg atcaactttt catgaccaca atgcaaaggc gcgaatttac caaccaccag
    57061 acccgttgca aaaggcttca tgatgtttgc aactctttgc gccattgata caaaccatac
    57121 caggcgttaa tccagaacag gaaatataag ccagcggtca ggtttaaacc gcgcgtcata
    57181 tacagtggca ccgccagggt attcaccgct aaccagacgt accagttttc gatacgtctt
    57241 cccatcaaca tgaattgtgc cagaacgctg aaggtcaaaa tcaacgaatc cagccagggt
    57301 gcccaagcat tggtaaaggt gtggagtaaa aagccgtaac cgcctgccac gacgacagca
    57361 cagagcaaca aaaggaaaaa gtgactggct tgcgttcggc gcaccggcaa gatgtcgcca
    57421 ccctgacctt tcagccagtg gatccagcca gtgatgctgg tcacgatgaa gaataactgg
    57481 agcgtaacgt cggcatagag ttgcacggac caaaaaaccc agccgtacaa tatgctgcca
    57541 attatgccga tccaccatgt atgcacattg tttctggcag ccagccagac ggaaaccgcg
    57601 tatgccagac acgcggcgat ttctgaataa gacattgtcc ctccctggag acgaatatta
    57661 atccacgtat ttcatcgcga ctcttgaagt caggcgcgta ataagttcgt aagcacttac
    57721 cttcgtcatt tcagcgatac gttctacggg caaaccttcg ccccataaaa tgaccggggc
    57781 cccggcttta tcctgcgcct gtggacctaa gtctacgcag atcatatcca tcgccacgcg
    57841 cccgacaatc ggcacttcgc gaccgttcac cagcactggc gtaccggacg gcgcggcgcg
    57901 cggataacca tcgccatagc ccatcgcgac tacgccaaga cgagtatcac gttcgcttac
    57961 ccaggttcca ccataaccga caggctctcc ggctttatgc tcacgcacgg caatcaggct
    58021 ggaggttagc gacatcactg gctgacagcc aaaatcggca ccggtggagc gatcttccag
    58081 cggagatacg ccgtaaagaa tgatgcccgg gcgcacccag tcaaaatgcg actgtggcca
    58141 gagtaaaatg ccacccgacg cggcaatgga acgttgacca ggcttgcctt cgcaaaaggt
    58201 attaaagata gcgagttgct tctcggttgc gccacatttt ggttcatccg cgcgcgcaaa
    58261 atggctgacg atattcaccg gctgacgaac gtttttgcac tgggtcagac gatgataaaa
    58321 cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga gtttcatcca
    58381 gacggtaacc ggctcgtcca ggttagcctc ttccagcgca gcaagctgtt cttcgttatg
    58441 cacggcggta tgaaaatgtt gcgcagaaat cgtcggtaga tctctggcat caaaaaagcc
    58501 ttcgagtaaa agtacaggtt tggtgattcc ccccgcacgc agtcgcagag cttcttcgag
    58561 acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa gaagaccgtg
    58621 accataagcg ttcgctttca ccaccgcaac cattttactg gcaggtgcca gttcacgaag
    58681 acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg cttgcatttg
    58741 tgttccttga taagtgtttg ctttaattac ctaattcata aaataattat tattcgtcgt
    58801 cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag gtcaggcgta
    58861 ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg ccttttaaat
    58921 cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc gcatcctgct
    58981 cgatagagcc agattcacgc aggtcggagt tgaccgggcg tttgtcggca cgttgttcca
    59041 gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc gccagtgctt
    59101 tcagcgagcg ggagatttct gcaatttcca gcgtacggtt atcggaaagc gccggtacgc
    59161 gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca cgggcaatac
    59221 ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga ggaatcatcg atatagatat
    59281 tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg tgcccaatct tcatcatcga
    59341 gctgcccggt acggatttta gtctggtcaa cacgcgacag cgacgccaga gaacgcatca
    59401 taatctgttc tgatggcatc tccagcgaga agataagtac cggtttatcc tgcaacatcg
    59461 ccgcgttttc gacgaggttc atcgcaaatg tcgttttacc catcgacgga cgcgcggcaa
    59521 cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatcg tcataaccgg
    59581 tgtttacccc ggtaacgcca tcgtgtggct gctgaaacaa ctgctcaata cgcgccacgg
    59641 ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgtttgca cgactttcgg
    59701 caattttgaa gacgcgggat tcagcaaggt ccagcagatc ttcactggta cgcccttgtg
    59761 gatcaaaacc ggcttcggca atctcattcg caacggagat catctcacgg acaacggcac
    59821 gttcgcgcac gatatccgca taggcactga tgttcgccgc acttggcgta ttttttgaca
    59881 gctctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt tccagcgatt
    59941 ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc atttcagtaa
    60001 agatatgacg gtgtgggcgg gtgtaaaaat cgtctgccac cacacgctcg gctacatcat
    60061 cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct tcgatcgagt
    60121 gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggttatca gtctgttgtt
    60181 tgttgaaagg tctattgcct gccataacga atggtgttac ctgctgttga atgggggaca
    60241 agagagtata gcgagaaaca gtttctcgct acagcattta acgcccccgc tttccggtgg
    60301 aagcgcgaat ttgcagggtc gggcgataga tgtaatggct gagcggtgcg tctggttgcg
    60361 caatacgccg taaaatttgc tgcgccgcct gtttccccag agccaatggt tctaccgcaa
    60421 ttgtggttaa cggcgggtta ctgtaagcac aagccgccac gccatcatta ccaatcaacg
    60481 aaatatcttc cccggcaaat aatcctcttt cgcctagtgc cgactccgcg ccaaaagcca
    60541 ttaaatcgtt ataacaaatc accgctgtgg gtggcgaagg catatccaac agctgttgca
    60601 tcaactggta gccgttggcc tgagtcggtt cagcacgcag cgaaaaagca tcagtactcc
    60661 acggctgtaa accatggtga ttcatcgcct caaggtatcc ctgataacgc atatggtgcg
    60721 taagaccacc aataaacacg atatctctgt gcccttgttg aatgagatat tccgtcgctt
    60781 cccgtacacc tgcgtggctg tccacgccaa cgtaatcgac gggcaattgt tgccaatcca
    60841 gagggcgcat cgtgattaat aagggtgtgg aactgttggc ataacgctgt aaatcggctt
    60901 ctgaggtact gttcactgga cagaggacaa tgcccgcgac gtggtgttcc agcaaggtat
    60961 caagcacctg tttttgccgg tcactacgct gggaggtatt ggacatcacg gtgataaaac
    61021 cgtgctccgc cagcgtcatc tcaagaccca tagtaaattc agcgaagaaa ggatttatca
    61081 aatcgtcaat gactacgccg atagttagcg atttttgcga acgcagattg gcagcaaaac
    61141 ggttatagac atagcctaat tgttcaatgg cttgctggac tttttgttgc gtctcttttt
    61201 tgataagtga actgtcgttg agaaccagcg atacagtcga tttcgaaacg ccagccgcac
    61261 gcgcaacatc ggttaatgtt acccgctttc ttcctgtatc cgacttcgcc atgcctgctc
    61321 caaaccccca taagatcaag gcaattactt tatcataatc tccttttact catgcgatcc
    61381 gttaacgcaa gggatcgcag gagatcgtcg tttcgatcac agattcgcac ttcccttgtt
    61441 gtgtttttta caccctaatc gtacaaatta cgcataaatt agatcgttcc aattaattta
    61501 tgaaaattat catattgttt taaatgtata tttttaatta tttttatatc gaaacaagga
    61561 aattattatg tgctctgtac gcacgatcac tgcaccggaa gctgcagatt tgattgttga
    61621 tggcgcagtt gtcaccgtct cctcctccag tggaatgggg tgtccggatg cggtgctggc
    61681 agcaataggt gaacgctttc aacgtgccgg acatccgcaa aacatcacct ccatccaccc
    61741 tatcgccgct ggtgatatgt atggcgtaaa aggcatcgat catctggcgc aaccgggctt
    61801 actggcaaaa gtgatcgctg gctcctaccc ctccggtcca tcatcaatga cgcctccgtt
    61861 aatctggcaa atgatcaatg acaacgcgat ccccgcctgg aatctgcctt ccgggatctt
    61921 atttgatatg caccgggaag cggcggctca tcgtcctggc gtgctgacac aaacaggcat
    61981 ggatacctat gtggatcccc tgttgcaggg cggtgcaatg aatgaaaaag cggcgcaaca
    62041 atctctggtt gaacgtgttc gatttgctaa cgacgactgg ctgtttttcc ccagtatcgt
    62101 gccgcaggtg gcgatcattc gcgctaccac tgccgatgaa cgcggcaacc tgacttatga
    62161 acatgaaggg gcttatctgg ggccactgga acaggctact gccgttcgca ataatggtgg
    62221 catcataatt gcgcaagtaa aacgacaggt tgccgccgga agtctgaaac caaaagaggt
    62281 gcgtattccg ggtgtactgg tggattacat cgtgattgcg ccggaacaaa cacaaacgac
    62341 acaaacgcaa tatgaacctg ccatttccgg cgaaatcagt cgtccgctgt cagcctttcg
    62401 ttatatggaa catggcccgg cgcgagttat tgcccagcga gtcgcgcaag aactacaaag
    62461 tggagatgcg gtaaatattg gctttggtat ttctgccaac gtaccacgca ttttactgga
    62521 acaaggtcgc cacggtgacg tcacctggct gctggaacag ggcgcgattg gcggagttcc
    62581 actgctggaa tttcagttcg gttgtgcctc taatgcagaa gcatttttgc cctctcccca
    62641 gcagttcacc tatttccagg gtggtggttt tgatctcact ctgatgtcgt ttttacaaat
    62701 cggcgcagat ggctcagtga acgtctcaca tctccccgct cgtccacatg tcactgccgg
    62761 atgcggtgga tttattgata tcacctcaca tgcgaaacgc attattttct ctggcttttt
    62821 caatgccggt gcccaactgc aactggaaga gggtcagctg cgtatcatta aagaaggcaa
    62881 agccaaaaaa ctggtacagg acgtcgccca cgtgacgttc tccggcaaac gcgcaataag
    62941 actcgggcaa caagtacagt acattactga acgctgcgtt ctggaattaa ccccagacgg
    63001 gctactggtc acagaaattg ccccaggtat tgatcctgaa cgcgacattc tcgcccaaag
    63061 cgatgcccca ctccgtctgg cagacgatct caaactaatg aatcccctct attttcaaca
    63121 aggaagtcac catgaataac ggcaccattg acctccagcg caacggtttc attgccactt
    63181 tggtgattaa tcgcccggcg aaactcaacg cattaacccc agagatgctc gaacaactgc
    63241 tggcgcattg ccacacgttg gaaactgatt cggagatccg cgtagtactg ctgaccagtg
    63301 ccagtgaaaa agcgttttgt gtcggcgcgg atattcatcg ctggacctct ctttctgcac
    63361 tcgatatgtg gcgcaactgg atccgccgcg gtcatcaggc atttgatgct ctggcacaac
    63421 tgccacagcc cgtaatcgcc ctgttgcacg gcctggctta tggcggtggt ctggaactgg
    63481 ctgctaccgc cgatatccgt atttgtagcg ccgaagcaca aatggcacta ccagaaaccg
    63541 ggctggcgac agtgcctggc tggtcaggaa cgcagcggct gacggcactg cttgggcgct
    63601 cactggtgaa agaactggta tttaccggtg aaccactgga tgcacagcgg gcactgtcct
    63661 gcgggctggt gaatcgcgtt gtcgccaggg aggcgttaca cgacgccgga ctgcaactgg
    63721 cacaacgtat cgcccagcgt gctcccgttg cagtgcagat cgccaagcag gttattgatg
    63781 ccggagaagg tattgctgtg gcctcgacgc ttgaagcact ggctggcgct ttttccgcca
    63841 cgactgaaga tgctcatgaa ggcagtcagg catttactga gaagcgtcaa ccgcactttc
    63901 aggcacgctg atcgcctctt acaaggaaag aaagataatg aatatgacaa cctctgttcc
    63961 ccaaacgcct tctttatttc ggcgaataat gacaaaactc gcggcctttg cacctggcat
    64021 ctttcttatt ggctacagca tcggaacagg cagcatcacc actatggcta aagcaggtgc
    64081 taactatggt gtctcgctca tgtgggccgt ggcgctttct tgcattgtta cctggttttt
    64141 aatccgtatt tatggtcgtt atgcttccgt cactggtgaa cgcgcgttga ctgcatttcg
    64201 tcgccatatc catcccggcg ttgcgatgtt gtttctgatc gggttaaccg ttgccgtgag
    64261 cggttccatt atgggggtaa tgggtattct ggcggatgta atggcaacat ggtccacgac
    64321 ctggtttagt ggcggtatct cagctctcgc ctgggctatc gcgatggtca ttatgattaa
    64381 cgtgatgctc tggaccaaca gcaacgccgg gttcgacaaa atcctctccg cgctggttgg
    64441 cattatgggg ctggcgtttg tgattaattt tttcattatg atgccgccgg tcgcagatct
    64501 gctggccgga tttattccgt ctgttcccga tcctgtcgct ggcgataaca gtacgccatt
    64561 tctgataatt tctggcatgg tgggtaccac cgtagcacca gcaatgtttc ttatgcgcac
    64621 gaccctggta aaaagtaatg gttggggtgt ggacaatttg cctcatcaaa atcgtgatgc
    64681 cattgtcagt gcaggaatga tgttcatcat cagtgcggcg gtgatggctg cggctgcggg
    64741 tacgttgtac caaactggca caggtttaaa taatgcctcg caaatggtca ttattctcga
    64801 acctattgct ggctcagctg ccgtggggct ctttgttatt ggtattgttg cagccgggct
    64861 ttccagccag ttcccaaatt tgctgttaac gccctggctg ctggctgact ggcgggatac
    64921 cagcaacgaa gttagcccag gttaccgtat cctgatgacg gttatgtctc tgctttgtct
    64981 ggttgtgccg ctgttccacg cacgcccggt attggtgatg attgccagcc aggcctttaa
    65041 cgccattatt ctgccgctga ctgttttatg tatgttctgg ctggcgaaca agccttcgtt
    65101 aatgggcaaa tatcgttgct cacgctggga aaacgtcatg tttattgcca taagcgtatt
    65161 tacgctaatc atgggctata tggcaatgag cggcttaatc gaaagtctga tgaaataggt
    65221 gggacgcaat gattgacgaa acgctaacgg cgctcactga tatcaaaatg ccccattacg
    65281 caccgctggc tcaagccagc ggtagtatcg accttaacgg taccatttta ccagagtact
    65341 gctgcaacac gctggttctg ggcagtggcg cggcaggatg gcgagctgcc gtcgagctga
    65401 aacggcaaaa tgtcgatgta atggtcgcct ccagcaaagc cttctggggc acttccgcct
    65461 gttctggttc cgataaacaa acgcttcaca ccgcgaatac gcgtgcaaat ggtgaccatt
    65521 ttctgacatt aagtaacacc ctggcggcag gtggtgcgat ggatcatgac accgcatatg
    65581 ttgaggcggt cggatcggtg aacacctgtg aagtactgaa atatctgggt ctggatctgc
    65641 ctgaagatct ctttggcgca acgttgcgtt atcaaaccga tcacgatgaa tttggccgtg
    65701 ccactagctg cggtcctcga acatcgcgcc tgatggtgaa ggtgctggcc caggaggcaa
    65761 tgcgccttaa tattcctctt tgcgatcaca caacggcgat tcatatcctg accagtggtg
    65821 aaggggaaaa tcgacgagtc gtgggggtga tcgccatcga taaagcctgt cgggataatc
    65881 cctggcgaat ggtcgtgatt cgctgccagc atctggtttt agccacgggc ggaccaggtg
    65941 aactttatcg tgacagtgtt tatccggtga actgttttag tgcgttgggc atggcactgg
    66001 aagcaggtat aactctggtt aatcttacgg aaagtcagtt tggtatcggt acaccgcgca
    66061 gccagtttcc gtggaatctt tccggtactt acatgcaggc gatgccgcgc atctattcgc
    66121 aggaccactc cgggcgtcag tataattttc tggcagctta ttaccccacg actcgtatgc
    66181 tggcctccgc aatatttcgt aaaggttacc agtggccgtt tcacgcggaa cgaatgctgg
    66241 aatatggctc cagcctgctg gatgtcgcag tttatgaaga gactcaaaaa gggcgcgatg
    66301 tttttcttga ttttctacgg gaaccagaac ccgcagcgga cggaacgtca ttcaacctgc
    66361 aagaactgga tgatgatgtc atcgactatc tgcaacaaaa tcacgccctg ttggcacaac
    66421 cgctggcgag attaacacag atgaatcctc tggctattcg tttgtatcag atgcacggtc
    66481 atgatctcac agcctcaccg ctcaagttta cgctaaataa tcaacatctt aatggcggaa
    66541 ttgaagttga tatttgggga cgtacatcgc taactggttg ttatgctgcc ggagaaaatg
    66601 ccggaacaca tggtgtcacg cgcccaggtg gagcagcgct caatgccggg caagttttcg
    66661 cccgccgctg tgcattacat attgctcatc agaaaagcca ggaccggcct ctcgagaggc
    66721 aacaaattct cagtacgatt aatgaggcgc aacagtacct gaatcagggg ctgctacgtg
    66781 acgacgtgcg agaaacgata caaaatacca tgagccacca tgccgcaatt ttgtgtcata
    66841 cacaggggat caacactgcc gcgcaaaccc tggcaacact ttgtgaagat attcgccggg
    66901 aaggtattca atgcgatgaa aacagcctgg cacaatcttt ccagtggcgg caaagtgcgc
    66961 aactggcgct ggctgtactg cgttctttac aatgttatgt cgaaaacggt ggtgggagtc
    67021 gtggtgccag ggctatctat gatggcgaag ccggtattgc accgcaaaca ccaggtgggc
    67081 cactcctggc ctggcgtttt cggccagaaa atcaggatgc caggcaatat atgatttgtg
    67141 tttgtcggga agctaatgat taccaaacct ggactcgccc gtgccgtgaa cggggcgtat
    67201 taacgctaac caactttgag cgccagtggc aaaactggat tgcggcgacg ccgtttcaat
    67261 actgatcaga gatattccgg gttgtgaccg cagcccggaa aaaatccccg cgcggcctgg
    67321 cgactactgt taggcttaaa acagatatgt cgctcagtaa aggagtttaa atggcaacgc
    67381 gcatcgaatt tcacaagcac ggtggcccgg aagtacttca ggccgtggag ttcactcctg
    67441 ccgatccggc ggagaatgaa atccaggtca aaaataaagc catcggcatc aattttatcg
    67501 acacgtatat ccgcagcggc ctttatccgc caccatcgct acccagcgga ttaggcaccg
    67561 aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa gcatattaag gcaggcgatc
    67621 gcgtagtcta tgcacaatcg gcattaggcg cttacagctc tatgcataac attaatgcgg
    67681 ataaagcggc gattctgcct gcggcaattt cttttgagca agctgcggca tccttcctga
    67741 aaggcttaac ggtttattat ctgctgcgca aaacctatga aattaaaccc gatgaacagt
    67801 tcctgttcca cgcagcggct ggcggcgttg gcttgattgc ctgccagtgg gcaaaagccc
    67861 tgggcgcgaa acttatcggc accgtgggaa ccgcgcaaaa agcgcagagt gcgctaaaag
    67921 cgggcgcatg gcaggttatt aactatcgtg aagagaatct ggtcgagcgg ttaaaagaga
    67981 tcaccggtgg caagaaagtg cgcgtggtgt acgattcagt gggcagagac acctgggaac
    68041 ggtcgctgga ttgcctgcaa cgccgcggct taatggtgag ttttggcaac tcatcaggtg
    68101 cggttaccgg tgtgaactta ggcattctca atcagaaagg ctcgttgtat gtgacacgcc
    68161 cttccctgca aggctatatc accacgcggg aggaattaac cgaggccagt aatgaactgt
    68221 tctctttgat tgccagcggt gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc
    68281 tgaaggatgc gcggcgtgcg catgagattc tggaaagccg ggcgacgcag ggttccagcc
    68341 tgttgattcc ataaaagaaa ttgggcttcc acctgggaag ccctttcttt tatagttcgg
    68401 ctgtatgtag ggtacagctc gatgaatttg tttgacacgc aatagtgaca gatttgatta
    68461 tcaattccta ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt
    68521 agtaacgcca gcggtcataa tgctgatatt tcggcacttt tggtgcttta atcgccctga
    68581 tcacccacac caccgcaacc gccaacagca gccacggcag caacttaatc atcaatgcca
    68641 gcataccgcc gaggaacata aaggccgtcg ccacaatcag cgcggcgata atacccagca
    68701 acgaaacacc agtgaccatc agcatgacaa aaaagccaat tacaaaaagt agttccagca
    68761 tgatgctctc ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat
    68821 ctattgattt aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac
    68881 tttttggtga aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacatta
    68941 atgtctgcac ccgctttatg ggcattttca cttaaataac gtcgccactg ccgcgcgcca
    69001 ggaataccct ggaacaagcc cagcatatgc cgggtaatat ggccgagata cgtcccctgg
    69061 ctgagttcac gctcaatgta cggatacatg gcgcgcacta ccgccaccgg atcggcatcg
    69121 gtatccgagg aaccaaaaat ctctcggtct accgccgcca gaatccccgg attctgatac
    69181 gcctcgcgcc cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc
    69241 gacttgatac caccgttaat cgacatcgtc agatgcggaa agtcacgctt cagttgatac
    69301 acacgcggat aatcgagcgg cgggatctca cggttttctt tcggacttaa cccagaaagc
    69361 caggctttac gtgcgtggat gatgaacatc tcacactcac ctttgccgga aacggtgttg
    69421 atgaaatcgc agagaaattc atagctgtcc tgatcatcga tgccaatacg cgttttcacc
    69481 gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca
    69541 ttacccatca gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca
    69601 ttcaggttga tctcatcata tccgcgcgct tctgccagct tcgcacactg tgccagcgcc
    69661 gccggatcgc tacccccgag ttgcaacgct accggatgtt cttcttcact gtacgccagg
    69721 taatcacctt taccgtgaat aatcgcccct gtggtcacca tttcggtata cagcaacgta
    69781 ttgcgggaaa gcagacgcag gaaataacgg caatgtctgt ccgtccagtc aagcatcggg
    69841 gcaatcgaga aacggtaagc gggataaaca gtgtgtgatt ctgctggcat ggcgactttt
    69901 taagcatctg gtaaattggg ggcgctacta tagcataacg aatatgcgat caggcaatgt
    69961 gacagccatg acaccctgtt ctcggcgttt aagtgagaac tatggtaaag taaggacatt
    70021 cttaacccca ctgtcgaggt gcccaatgga aaagaccaca acgcaggagt tattagcgca
    70081 ggctgaaaaa atctgcgcac agcgtaatgt gcgcctgacc ccacagcgtc tcgaagtgtt
    70141 gcgcctgatg agtctgcagg atggcgctat cagcgcttat gatctgcttg atttgctgcg
    70201 cgaagctgaa ccgcaagcca agccgccaac ggtttatcgc gcgctggagt ttctgcttga
    70261 acaaggtttt gtgcataagg tggaatccac caacagttat gtgctctgtc atctgttcga
    70321 tcagcccacc catacttcag ccatgtttat ttgcgatcgc tgcggcgcgg tgaaagaaga
    70381 atgtgctgaa ggcgtggaag acatcatgca tacgctggcg gcaaaaatgg gttttgccct
    70441 gcggcataat gttattgaag cacatggatt atgttcagca tgtgtagaag tagaagcgtg
    70501 tcgtcatcct gaacagtgcc atcatgacca ctctattcag gtgaaaaaga aacctcgcta
    70561 aaagggtgta catccttgta catgtcgggc aggagggatt aattaccagc ggtaatcatg
    70621 gcgtttttcc caactatcaa cctctttttc tgcctgatct ttctgataac cgtagcgttc
    70681 ctggatttta cccactaact gatcacgttt tccttcaatg atcgtcatat catcatcggt
    70741 cagtttgccc cattgctctt tcactttacc tttaaactgt ttccagttac cgccggcttc
    70801 atctttattc ataatcaaga cctcatcgtt aggttgtgaa tgagagtacg ttcacttttc
    70861 ttctgaacgt gagattaagt gtagtcagca atttggctta ggattattta ttcagaattt
    70921 ttaaccgtca cgttgcgaca aaccaggtat cgttacgcca gtgacgccgc cagatagccg
    70981 ccagagaaag cccgcgtaac gccagaaaga cggttaacgc cagccacaaa ccatgattcc
    71041 ccagccacgg cagcgtaagg agcgtcagtg caaaacctgc ggcggccacc gccatactgt
    71101 tacgcatttc ggcggcacgc gttgcgccta taaacatgcc gtccagcaga taacaccaga
    71161 cgccgaccaa cggcaaaatc acctgccaga taagatagcg gtcagccagc tgctgaatct
    71221 gggttaacga cgtcagcaac gcaatgatgt gttcccctgc cagcaaataa accaccgaaa
    71281 acagtaacgc tacgatcccc gactggcggc acgctgcccg ccagacatcc agcaattgac
    71341 taccgtcgcg cgcaccgtat gcctgaccgg aatgtgcttc aaccgcgtag gcaaaaccat
    71401 ccagcgcata ggcggtaaag gtgagtagcg tcatcagaac cgcgttaaca gcgataatgt
    71461 cactccccag tcgcgcgcca agtacagtga tcgcgccgaa acagagttgc agcaacagcg
    71521 agcgcagcat gatatcgcgg ttaagcgcca gcaagtgacg gaagtttcct cgccaggctg
    71581 ttttcagcat ttcgccggag attccgcgta gtttgagaat tttacgcacc attagcagac
    71641 caatcagcaa tgttgcatat tccgcaataa ccgtcgccag agccgcgccc tgcacgttca
    71701 tatgcagccc catcaccagc cagacatcca gcacaatgtt gaggatattg ccgaccacta
    71761 acaaaattac tggcgcacgg gcatattgca cgccgagtaa ccagccaagt aataccagat
    71821 tcgccagcga cgccggtgcg cttaaccagc ggatttcaag aaagcgtcgc gcctgttcca
    71881 ggactgcttc actaccacca acaatatgca gcgccagatc gataatcggc gtacgcagca
    71941 gcgcaattaa cgccccggcc cccaacgcca acagcaacgg ttgcaccagc gcacgggcta
    72001 atgcctgagg atttttggca ccataagcct gtgcagtcag cccggtggtg ctcatgcgta
    72061 aaaacagcaa caacataaag agaaagctgg tcgccgttgc accaaccgcc acgccgccta
    72121 aataaaccgg actatcaaga tgaccaatta ccgccgtatc gaccagtccc agcaacggaa
    72181 cggtgatatt ggagaaaatc atgggtaagg cgagatgcca gagtgcttta tcagatgaag
    72241 tgaggaatgc catgcagaca agcctgatga agagagatga aaaacaaacc gcgacgccag
    72301 gcggcatcgc ggtctcagag atatgttaca gccagtcgcc gttgcgaata accccaaccg
    72361 ccagcccctc aatggtgaag ctctgctgac gaaggtcaac gacaattggt ttaaactcgc
    72421 tgttttctgg caacagttcg actttattgc cctgtttttt caggcgttta acggtaactt
    72481 cgtcatcaat acgtgcgaca acgacctgac cgttacgtac atcctgagtt ttatgcactg
    72541 ccagcaaatc gccatccata atgccgatat ctttcatcga catcccgctg acacgcagca
    72601 ggaaatcagc attcggcttg aacaaggaag gatcgacctg ataatgacct tcaatatgct
    72661 gttgcgccag aagcggttca ccggcagcca cacgacctac cagcggcaac ccttcttcct
    72721 cttcctgcaa taaacgaatc ccgcgtgatg cgccggaaac aatttcaata acgcctttgc
    72781 gtgccagcgc cttcagatgt tcttcagccg cgtttgggga acggaacccc aaacgctgcg
    72841 cgatttccgc acgcgtcggc ggcatacctg tctggctgat gtgatcacgg atgagatcaa
    72901 acacctcttg ttgcctggcc gttaacgctt tcattccgcc ccctgggtgt atatacagtt
    72961 atgctgtgag tatatacagc aaaaggcgat tttggaacca taaactgcac aataaaccag
    73021 agatttatcg aaatctggaa gggttatcca aaatgcgacc ataacaggat gcaccaggta
    73081 atcacggcga cgataatggc aatcagcact gcggcagatc ccatatcttt agcgcgtccg
    73141 gaaagctcat ggtattcaga gccaattcgg tcaaccacag cttcgatggc gctattgaga
    73201 atttccacaa tcatcaccag catcacggag ctgataagca gcacgcgggt aatcgcgtcc
    73261 acatccagcc agcaggcgat aaccaccgct aacaataccg ctacgccttc ctgacggaat
    73321 gccgcttcgt tgatccatgc agcgcgtaaa cctttccagg aatagccagc agctttaata
    73381 attcggatga atccagtggt attattggcc attgaaagaa cctttttaca ttatgagcgt
    73441 caatatcagt gtaccgttaa atccgatgat tcgggtttgc gtaacgccgc gcgaaacatg
    73501 agcggatacc acagaatttc ccatgacttt ctgctatcct tgccgcgcat ttgcattatt
    73561 aaccagaggc tttacatcgt ttatgtccgg ctggccacga atttactaca aattactgaa
    73621 tttaccatta agcatcctgg taaaaagcaa gtctattccg gcggatcctg ccccggaact
    73681 ggggctggat acctctcgtc caattatgta cgttttaccg tacaactcga aagctgactt
    73741 gctgacgttg cgcgcccagt gtctggcaca tgacctgcct gacccgttag aaccgctgga
    73801 aatcgacggc acgctactgc cgcgctatgt gttcattcac ggcgggccgc gtgtgttcac
    73861 ctattacacg ccgaaagaag agtctattaa gctgttccac gactatctcg atttgcaccg
    73921 cagcaaccca aatctggatg tgcagatggt gccagtatcg gtgatgtttg gtcgcgcgcc
    73981 ggggcgtgaa aaaggcgaag tgaacccgcc gctgcgtatg cttaacggcg tacagaaatt
    74041 tttcgctgta ctgtggctcg gtcgcgacag ttttgtgcgt ttctcgccgt cagtttcact
    74101 gcgccgtatg gcggatgaac acggcacgga taaaactatc gctcagaaac tggcgcgcgt
    74161 ggcgcgtatg cactttgccc gtcaacgtct ggctgccgtg ggtccacgtc tgcctgctcg
    74221 tcaggatctg tttaataagc tgctcgcctc ccgcgccatt gcgaaagcgg tagaagatga
    74281 agcacgcagc aaaaaaatct cccatgaaaa agcgcagcag aacgcgattg cgctgatgga
    74341 agagattgcg gcgaatttct cttacgagat gatccgcctg actgaccgca ttctgggctt
    74401 cacctggaac cgactttacc agggtatcaa cgtccataac gccgagcgcg ttcgccagct
    74461 ggcccacgac ggccatgagc tagtgtatgt gccttgccac cgcagtcaca tggactacct
    74521 gctgctttct tacgtgctgt atcaccaggg gctggtgccg ccgcatatcg ccgccgggat
    74581 caacctgaac ttctggccgg ccgggccgat tttccgccgt ctgggggcgt tctttattcg
    74641 ccgtacgttt aaaggcaata aactttattc caccgttttc cgtgagtatc tcggcgaact
    74701 gttcagccgt ggttattccg tcgagtattt cgtggaaggc ggtcgttccc gtacggggcg
    74761 tttgctggat ccgaaaaccg gtacgctatc gatgaccatt caggcgatgc tgcgtggcgg
    74821 cactcgtccg attacgctga ttccgatcta tatcggttat gagcacgtca tggaagtggg
    74881 tacttacgcc aaagaactgc gcggtgcgac gaaagagaaa gagagcctgc cgcagatgct
    74941 gcgcggttta agcaagctgc gtaatctcgg tcagggttac gtcaacttcg gtgaaccaat
    75001 gccgttgatg acctacctta accagcacgt accagactgg cgtgaatcta tcgatcccat
    75061 cgaagcggtg cgtccggcct ggttaacgcc gacggtcaat aatattgctg ccgatctgat
    75121 ggtacgcatt aacaacgcag gcgcggcgaa cgccatgaac ctgtgctgta ctgcgcttct
    75181 ggcgtcacgt cagcgctcac tcacccgcga gcagttaacc gagcaactca actgctacct
    75241 ggatctgatg cgcaacgtgc cttactccac ggactctacc gttccttcag ccagcgccag
    75301 cgagcttatc gatcacgcgc tgcaaatgaa caagtttgaa gtcgagaaag acactatcgg
    75361 cgacatcatc attctgccgc gcgagcaagc ggtgctgatg acctactatc gcaacaacat
    75421 tgcgcatatg ttggtgctgc cttcgctgat ggcggcaatt gtcacccagc atcgccacat
    75481 ctcccgcgac gtattgatgg agcacgtcaa tgtgctttac ccaatgctga aagcggaact
    75541 gttcctgcgc tgggatcgcg acgagttacc ggacgttatt gatgcgctgg caaatgagat
    75601 gcaacgtcag gggctgatta ccctgcaaga tgatgagttg catatcaacc cggcgcattc
    75661 tcgcacgcta cagctgctgg ccgcaggcgc gcgcgaaacg ctgcaacgtt atgccatcac
    75721 cttctggctg ttgagtgcca acccgtcgat caaccgcggt acgctggaaa aagagagccg
    75781 caccgtcgcg caacgtctct ccgtgctgca cggtatcaac gcgccggaat tcttcgacaa
    75841 ggcggtgttc agttctctgg tgctgacgct gcgtgatgaa gggtatatca gcgatagcgg
    75901 cgacgccgaa ccggcagaaa cgatgaaggt atatcagttg ctggcggagt tgattacatc
    75961 agacgtgcgt ttgacgattg agagtgcgac gcagggcgaa gggtaatcag tgagaattgc
    76021 cggatgcgac gcaaacgcct tattcggcct accatgatct gcaaattcaa taaattgcga
    76081 tatgttgtgt aggcctgaga agcgcagcgc atcaggcaac ccagaagaaa gccggatgat
    76141 catccggctt ttttacatca tcagaaatgc cagtaactca ttgccagccc taaaaacagt
    76201 accagaccaa catagttatt attcataaat gctttaaagc aggcttcacg ctcgcggttg
    76261 gcaatcagtt tttgttgata aacaaacagc gcgccagcta ccagaattga ccagtaatat
    76321 ccccagccta agccatttaa ctcaccgatg atcgccatca gcgctagtac gccaatctgc
    76381 aaaataccaa taatcaattt atcgtattgg ccgaacagga ttgcggtgga tttaatgcca
    76441 atcttcacat catcatcgcg gtcaaccatc gcatactgcg tgtcgtaagc caccgcccag
    76501 agaatattgg cgaggaacat taaccagcaa ctcaatggca ccgactcact cacagcggca
    76561 aaagccattg gaatcgacca gccaaacgcc gcgcccagca ccacttgcgg tagatgggta
    76621 taccgcttca taaacgggta tacccacgcc agcgccagcg cggcaattga caacagaata
    76681 gtcatcgtat tcagcgtcag caccagtaaa aatgaaatca gtaccagcac tacaaacagc
    76741 gtacgcgctt ctttctctgt caccgcgccg ctgggaagtg gtcggttcgc cgtgcgctta
    76801 acatgaccat caaacttgcg gtcagcataa tcattcacca cgcaaccagc agcgcgcatc
    76861 agccagacgc ccgcgacaaa caccgccagg atccagagct ggggaacgcc cggtgtcgcc
    76921 acccacaacg cccataatgt tggccagagc agcagtaacg cgccaattgg cttatccgta
    76981 cgcattaagc gatgaaacgc cagcagctta ttctgcgtca gactccactc catatttttt
    77041 tcctcttagt acaacggtga cgccggtaaa aacagttctg ttagcaacag cggtttaccg
    77101 cttaatcgca ggcgggaacg tcgcccccac agtccggcat cacggcctat ctcaataaag
    77161 tcccgggtta atgtcgatga tgtgaacaga tagcgtccta acggcgtttt acctaatttt
    77221 tgtaacgcca gctccggccc gcttaacgtt gacacaggaa cgacggtacg accggcaagc
    77281 cacggttcac catcggcaca taacaaaatt tcacgtaacc agtaacgaga ctctttcggc
    77341 agcagtggca gttcttcggg gatttcattc tgctcgacaa acccttcgcg gatcatcgtc
    77401 acgctcaccg tttttccctg ctgttcaaaa cgttttgtca tggaatcttc cagcaacagc
    77461 cagtcgagca gttgcggctc cagggcaggg atctctgtaa aatagcgcag cgcacgcagt
    77521 tgcgttaacg cggggtgtga catgccgaac tctccgttac ataacgtaaa ggcattgtat
    77581 cgcagaaaaa tatttacggg agatgtaaag caaattaagt gaactaatgc gcaacaaaaa
    77641 cgcaacaata gtacaggagg agaaaaaaag gtgcgccagg agacgcacca gttgttgcaa
    77701 aatcagcact gtggagcgta attacccctt gccttttaca ctgctgataa aggtggaacg
    77761 ggcagatgtc gaccccaggc gttcagcttc atcaagcagt tttaacgcct tatcaacatc
    77821 acctttcgcg acagcgtttt tgatcgcggt attaaaataa ctttccgtgt cgttgagcat
    77881 cggctcgctt ttcttcaccg gtgccggagc gggtgcagcc acagctggtg ccgccgtgtt
    77941 acctaccgta accggagctg gagcggaaga accaaatagt ggtcctacca acacgctgga
    78001 gctggagttc gttttcactt tcagtttcag caaaccatca gtggtgtgac gggcaatcgg
    78061 gtcaggaata tccggcaccg agttacctac gcctttggca taggcttttg ccgggtcgag
    78121 cagttgggtt gtctgctgga gatctttttc cgtggtgaag accagtacat agagtttctg
    78181 ttgccccagt gccggtgtca ggcgcataac gccttccaga cgatctgcac tcatcacgcc
    78241 gggttcctgg taggtgaaat aactgctggg gaagaaggct gatggggtca tgttctgatc
    78301 aagaatcagc acgttcggcg caaatacgct ggtttgtttg ttcacttcgc tggtcagcgt
    78361 cagtgtcagt tcaccaatgt ttgccgggac gctgtacgca gcaaccggac cactgatgcc
    78421 ggaaacgttc agttgttggc cgccggtcga cagttgagtc gtctgggttt tagattgatc
    78481 gaccggtgtc caggtgagtt gttgcagcgc ggcagatgga atggctggcg cgtcgctggt
    78541 gttttgcggt acgtagttaa catcggcaag gctgattcca ggtgcgcttg ccagcaaccc
    78601 tgctgataaa cagagagcga tgagactttt attcattttc attgttttca cctcaaaatc
    78661 tggagctcag cggtagccag gcaatagcgc gctaaacccg ataatcagag gggctttcgc
    78721 cccttaaagc tgtttttatg ccggatgcgg cgtaaacgcc ttatccggcc tactttcggc
    78781 agcggttgta ggcctgataa gacgcaacag cgtcgcatca ggcattgatt gccggatgcg
    78841 gtgcaaacac cttatccggc ccagtttttt gctattacca ccagatttcc atctgagcac
    78901 cgaaggtcca ctcgtcgctg tcgccacgac cgaagctacc gccattgaaa tcagccgaca
    78961 cagccttgcc gtagtatggg ttggttgaag agctaccggt gtagtcataa ccccatttct
    79021 catcccactt ggcgtaggtt gcgaagacac gaatagccgg gcgtgaccag atgctgtcgc
    79081 cagcctgcca ttgttgtgcg agggtaattt tgtactgatt gttcttgtcg ccggtgcgct
    79141 gggattcgac gttgtcgtag ccgatttcca tcacggtgct catgattggc gtccacttgt
    79201 acatcgggcg aataccgacg gtccaccact tggtgccgtt gtcgttatcc cagttgatat
    79261 cctggtacat acccacgtac atcatgtccc agttgtcgcc catggagatc gcaccgtggt
    79321 cgaggatacg cagcatgtga ccgttgttat tgatattgta ggcaaatttt tcgttatcaa
    79381 acgcgacgcc agaaccctgc gacagacctt taccctgcga ggtcatcgag tcagtagcgt
    79441 actgaacaac aaacttgtta aagcccttca ggacactctg agtatgttca gcagtgaata
    79501 accagccgtc tttcgatgcg ccatcaacca gacgatagtt atcacgcagg ttggcacgac
    79561 cgtagtcgac acccagttct aatgtgccgc ccgggttgat ttccatctgc gctaaacgca
    79621 catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata aatattgttg ctggcgaaag
    79681 aggaagaacc ccccgcttca gaggagcggg ttgctgccag agagagttta ccgaagccaa
    79741 catcgatgtt ttccagaccg gcaccaggac cagaaatatc ccagtagtag aagtcgatca
    79801 tatgaacgtc atgacgttgg tagaagcgct tacctgccca gatggtggag cctggcagcc
    79861 attcgatcag gtttttaccc tgcacgtttg cttcacggaa agccggatcg gtagcttccc
    79921 agtcattctg ttgcgcgacg gaataggcca cgttagtgtc gaaatagaag ctcttatcgc
    79981 cctctttcca cacttcctga cccaatttta attcagcata agtttcacat tcgttgccaa
    80041 gacggtattt actttgagca ccggtagtct ggaaacactg ttgttcgccg ccgctacctg
    80101 tccagccaat accggaacgt gcatagccgt ggaaatcaac agccattgcc tgagcagaca
    80161 ttacgcccgc tgcgacggca accgccagag gaagtttgcg cagagtaatc atcattctat
    80221 ctcctgagtc attgcttttc ttttttcaca tcacctgtga caggctttgt gtgttttgtg
    80281 gggtgcttaa acgcccggct ccttatgcag tcgacgacat gcagtgccat cctcacggaa
    80341 cagatggcaa cgctctggcg gcaggccgat agcgaatgtg gcaccttctt ctaccaacac
    80401 cacgtcgttc tggcggtaca ccaggttttg acgaatggaa gggatctgga tatggatttg
    80461 cgtttcgttg ccgagttgct cgacgacctg aacttcaccc tcaaggatga cgtcagcgat
    80521 atcactcggc agtagatgct ccgggcgaat acccagcgac atattggctc ccacctggac
    80581 atcacggctt tcgaccggta gccagacttg ctgacgattc ggcatcggca gctccacctg
    80641 cacttgatcg attgcggtgg cggtcacttt tactggcagg aagttcatct ttggcgaacc
    80701 gataaatccg gcgacaaaac ggtctgccgg atagtgatac agctccagcg gtttcccaac
    80761 ctgcgccacg cgaccagcgt ccagcaccac gattttgtcg gccagcgtca tcgcttcgac
    80821 ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt ttatgcagac gggagatttc
    80881 gatacgcatt tgcacacgca gtgcagcatc gaggttggag agcggttcat cgagcaaaaa
    80941 tacgcttggc tcggccacca gcgtacggcc aatcgccaca cgctgacgct gaccaccgga
    81001 gagcgctttc ggtttgcgat ccagcaaatg cgccagttgc agcacttccg caacctggtt
    81061 aactcgctgg ttaatcactt cttttttcgc gccagccagt ttcaggccaa atgacatgtt
    81121 ttctgctact gacaggtggg gatagagcgc gtaagactga aacaccatac caacgccgcg
    81181 ttctgcaggc ggagtgtcat tcatccgttt ctcaccgatg aacaggtcgc cgctggtgat
    81241 cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta ccgcagccag acggtccgac
    81301 aaacaccacg aattcacctt catggatatc gagattgata tctttcgata ccacgacctc
    81361 gccccaggct ttcgttacat tttgcagctg tacgctcgcc atgcccttct ccctttgtaa
    81421 caacctgtca tcgacagcaa cattcatgat gggctgacta tgcgtcatca ggagatggct
    81481 taaatcctcc accccctggc ttttttatgg gggaggaggc gggaggatga gaacgcggct
    81541 tctgtgaact aaaccgaggt catgtaagga atttcgtgat gttgcttgca aaaatcgtgg
    81601 cgattttatg tgcgcatctc cacattaccg ccaattctgt aacagagatc acacaaagca
    81661 acgatggggc gtaggggcaa ggaggatgga aagaggttgc cgtataaaga aactagagtc
    81721 cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga aaataaaaac
    81781 aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg cctcggctct
    81841 cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
    81901 tctcgctgaa gtcggtaaga aattcgagac agataccgga attaaagtca ccgttgagca
    81961 tccggataaa ctggaagaga aattccctca ggttgcggca actggcgatg gccctgacat
    82021 tatcttctgg gcgcacgacc gctttggtgg ctatgctcaa tctggcctgt tggctgaaat
    82081 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
    82141 caacggcaag ttgattgctt acccgatcgc tgtcgaagcg ttatcactga tttataacaa
    82201 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
    82261 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccatact tcacctggcc
    82321 gctaattgcg gctgacgggg gttatgcgtt caagtacgaa aacggcaagt acgacattaa
    82381 agacgtaggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgatctgat
    82441 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
    82501 aggcgaaaca gcgatgacta tcaacggccc gtgggcatgg tccaacatcg acaccagtaa
    82561 agtgaattat ggtgtaacgg tactgccgac cttcaaaggt caaccatcta aaccgttcgt
    82621 tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg cgaaagagtt
    82681 cctcgaaaac tatctgctga ctgacgaagg tctggaagcg gtcaataaag acaaaccgct
    82741 gggtgcagta gcgctgaagt cctatcagga tgaacttgcg aaagatccac gtattgccgc
    82801 caccatggat aacgctcaga aaggtgaaat tatgccgaac atcccgcaga tgtccgcttt
    82861 ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtggatgc
    82921 tgcgctgaaa gatgcacaga ctcgtatcac caagtaatgc tgtgaaatgc cggatgcggc
    82981 gtgaacgcct tatccggcct acgaaattga aacgtatgta ggcctgataa gacgcgtcag
    83041 cgtcgcatca ggcagttgtt gtcggataag gtatgaaagc cttatccgtc ctggaatgag
    83101 gaagaacccc atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg
    83161 gtcagtgtta ggtctgctcg gcctgctggt gggttacctt gttgttttaa tgtacgcaca
    83221 aggggaatac ctgttcgcca ttaccacgct gatattgagc tcagcggggc tgtatatttt
    83281 cgccaatcgt aaagcctacg cctggcgcta tgtttacccg ggaatggctg gaatgggatt
    83341 attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact acagcagcac
    83401 taaccagctg acttttgaac gtgcgcagga agtgttgcta gatcgctcct ggcaagcagg
    83461 caaaatctat aattttggtc tttatccggc gggcgatgag tggcaactgg cgctcagcga
    83521 cggcgaaacc ggcaaaaatt acctctccga cgcttttaaa tttggcggcg agcaaaaact
    83581 gcaactgaaa gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc gcgtaattac
    83641 ccagaatcgt caggcactga gtgacattac cgccattctg ccggatggca acaaagtgat
    83701 gatgagctcc ctgcgccagt tttctggcac gcagccgctc tacacactcg acggtgacgg
    83761 cacgctgacc aataatcaga gtggcgtaaa atatcgtcca aacgaccaga ttggttttta
    83821 ccagtccatc actgccgacg gtaactgggg tgatgaaaag ctaagccccg gttacaccgt
    83881 gaccaccggc tggaaaaact ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt
    83941 cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc
    84001 ggtcggcatg gttctggcgt gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta
    84061 tcgcgtcctg ctgattctgc cctacgcggt gccatcgttc atttcaatct tgattttcaa
    84121 agggttgttt aaccagagtt ttggtgaaat caacatgatg ttgagcgcgc tgtttggcgt
    84181 gaagcccgcc tggttcagcg atccgaccac cgcccgcacg atgctgatta tcgtcaacac
    84241 ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga
    84301 cgatttgtat gaagcctcag caatggatgg cgcaggtcca ttccagaact tctttaagat
    84361 tacgctgccg ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa
    84421 ctttaacaac ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc gtcttggcac
    84481 gaccacgcca gccggttata ccgacctgct tgttaactac acctaccgta tcgcttttga
    84541 aggcggcggg ggtcaggact tcggtctggc agcagcaatt gccacgctga tcttcctgct
    84601 ggtgggcgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg attaagggag
    84661 ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag cacgtttgtt tattactcac
    84721 ctgctactgc tactttttat cgcggcaatt atgttcccgc tgctgatggt cgtcgccatc
    84781 tcgctgcgtc aggggaactt cgcgaccggt agtctgatcc cggagcaaat ctcctgggat
    84841 cactggaaac tggcgttagg ttttagcgtg gaacaggctg atggtcgcat tacgccaccg
    84901 ccattcccgg tactgctgtg gctgtggaac tcggtaaagg tcgccgggat ttccgcgatt
    84961 ggcattgtgg cgctctccac cacctgcgcc tacgctttcg cccgtatgcg ctttccaggc
    85021 aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt tcccggcagt actttcactg
    85081 gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca ttccattcat tggcctgaat
    85141 actcacggcg gcgtaatttt cgcgtatctg ggcgggattg cgctgcatgt ctggaccatc
    85201 aaaggttatt tcgaaaccat cgacagttcg ctggaagaag cggcggcgct ggatggtgcg
    85261 acaccgtggc aggccttccg ccttgtcctg ttgccgctgt cagtaccgat tctggcggtt
    85321 gtgttcatcc tgtcgtttat cgctgccatt actgaagttc cggtcgcgtc gctattactg
    85381 cgtgacgtaa acagctacac cctggccgtg gggatgcagc aatacctcaa cccgcaaaac
    85441 tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg cattaccgat caccatcgtc
    85501 ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg caggtggtgt gaaaggttaa
    85561 agatgttgtt ctgccaatgt tatgccgctg caccctcaac ttacgttatc ccaacttgtg
    85621 actgttattc ggcgctccac ggagcgcctt tttttactcc cgcttcagcc gtttcgaatt
    85681 acacaaccac agggtgatca ccagtaacag gatcgctgcc gagtagatca acacatccag
    85741 tggcgattta tgatcgacga tgatcaagcg cacaatggca gtgatcccaa tatagacaaa
    85801 gtaacgtagc gggaagtgaa aaccggactg aaagtacttc acaatcagcg cgataaattc
    85861 gaaatagaga aagtaaacca ccagaccttc taccagctca tatttactgg tttgttctgg
    85921 cgcgaacagc acatcagcca gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag
    85981 gccgaggcac aacaggccaa gattgagtac ggtctgcaaa atggtggaga taaactccac
    86041 gcgcggacga gagagtgacg tcatagcagt acctccttca cagtggcgag cttcctgtat
    86101 aacgcaaaaa tgtgacggag atctatattt tttgtgtatg ttttgttcag ctcaattaag
    86161 tgggcatctc ccatcaatgt ctcatctggc aaaacacaca gcccaaaaaa atcacccatt
    86221 caggtgattc ccagccacca gcaatgcgcg aaaaatcgac aatgttcctc tggaacaaat
    86281 gaaacgtaac ctgataaagg tggtaaaaat gaagccttat tttgctgctt tgatgttatc
    86341 agtctctgtt cttcctgctt atgctggtcc gctcggtact gctgataaag ccgacctacc
    86401 gcaaagcaat gtctccagcc caatgatggc ccagtcgctc aggcaaccag acctgcaacc
    86461 gatatcgacg gacaggaaga cagaatgttt tcgcctttac acaccagatc gcaaaccggg
    86521 ggtgaactgc gttcctgatg gctcgacagg ccattaagaa taaaggtgcc agtgagtgcg
    86581 aaacctggca cctttcccat gacttagcgg aaattgacgc tacggtcgct ggtcatgtca
    86641 tacaactgga acttacgccc aagttgctga ccaccgtcac gcgtcagcgg cgtccagcca
    86701 atcgccgcac ggctgcgtgt cgggccagac gagaagagat ccagcggtac agagacatac
    86761 acgcctttgg tgaagtcccc ttcgccgtac tcctctttcg aaacattcgt gatcgtggca
    86821 tagccgccaa ccaccacgcc gctgtcaaag cgtttagcga tctccagcgt gccgccttta
    86881 tccccagcca gatactgccc gacgctcgct ttcactaaca cgtcctgggc gaaagatggc
    86941 gtccagtacg cggtcagatg tccggttttc acgctgtaat cggtgaattt catcatatct
    87001 tttgcgctac gccagtcgcg ctgtttaacg tagttggcat ccagaccaaa cgcccagttg
    87061 ctatccagcg ggcgatacaa cacttctgcc cccacgccgc caaacatggt ttcgagataa
    87121 cctccgtaga cctgaccgta gaagccgttg cccagatgct ggaagtagtt ggcttgcagg
    87181 ttattcacat agacatcatt ctgcacatac tcgcgcacat gggtacgcac gcgcggtaag
    87241 tgcgagtcct gcggtggatt ggtgtagtta aacttgtcgt agttgttggc aaggttagca
    87301 aacaggctgc cagtggtcag cagatggtcc gtcagccaca aatccgccgt tcccatcacg
    87361 cccagctgat acatgtaaaa gttttccggg ccaccgatcg actggttcag caccggatcg
    87421 atatggaaat caaagcgtga tttgtcgata taccagccct gctcggtgga cttcggaacc
    87481 actggctcga cgcgtttttg cgccagcttc gtttcgtggc ccaacggctc tccggcgaga
    87541 tgatttttca ggctggcgac atcggtttcc gtcgtcacct gtggcatatt aaggcgattt
    87601 tccgtaatgc ggatcgtttt tatcccatcc ggcagatcgt tcatcacgat ccgattagca
    87661 cggatgatcc cttcgcgcgg atcacgatat ttcacctgct cgccggtaac atacagcgta
    87721 tcgcctttcg cctggatctg cggatcggca agtccggcat tgtatttcaa cagcgttaac
    87781 tgattcgcca ccaccgaatg ctgcaaaatg gcatcctgcg gctgcggttg atattgcggg
    87841 cgggcgttat cgttgtaaga cgggcgcaga tcgttaaagt tggtgcgcag cgtgacgcca
    87901 aacataaaag tgttgccacg ttcatagcta aggttaacgt cggcccaatc ggtaacgcga
    87961 taaatcgccc cgacgttaaa cttacttttt tgctccagct ttccggcaaa atcctgctga
    88021 taattattgc cttcatactc cagtttcaga cgcagcggtt gccagggcgt ctggtattcc
    88081 acgccgccaa acagtgaggc cgggccgtgg aacatctggc tgccgtcgat agatcccgcc
    88141 tgtttgtagc tgttatcgcg atagcaatat ttatcactgg ctgaacagag cggatttttc
    88201 acgttaccgc tggtgcccag atatccccag cccaggccga gtgtaaaatc gaacggcccc
    88261 caggctttgc tggcaacaag atattccgca tcaaacagcc ccgtaccgcc gatatcccgc
    88321 gcaccaaccg ccacttgcgg cagccaataa ctctcttccc acaaacgcag tttgagatcg
    88381 aaggctttat ctttatacgt ttgatcgcca gagaacgctt cgacgctgct gtactgccgg
    88441 gtgcgcacgt cggtgtagcg cagcgttgtt tccagccacg ggaagagttg cactgaagct
    88501 gagtaataac ggtactgatc gttatcgcga tagttcaggc tcagctcccc ttcccgcgcc
    88561 atgcgcgcgg tgggcgtttg taataatcct acgccaccga aatccgattg cgacggacca
    88621 atgggcgcag gatatgtttc gccgtagcaa gctgtgctaa tacccagcgc cagtaagcta
    88681 agcagatgtc tttttttcat tattggggta tccgctgcgt cagagtctga agaatgtcgg
    88741 cgttaagggc atcaggtgtc tcactccaga cggagtccgc gaggccaaca taaataatgc
    88801 tgccgggcat cggctctacg tgacgcttgt tccagtaagc caccggcgct ttttgcgtgc
    88861 gtccgtccgg gtaaaccacc cacgcgtagc tgcgatccgc accgctgagc aggctctggt
    88921 cagagagata gctcgccacg tcgcgacctg gagtgaatga ctgcttgcca ggacgactga
    88981 taagcccgaa caacgtgacc gtggacggcg gcggtccgac ccacagcgtg tagttgccct
    89041 gcaacggcgg gttaccgcgt tcggcaacgc ggacgatatc aggatcaaga ttgatttttt
    89101 gcctgcccgt cactttcaac gcctgaattt gctggcgtaa ggcgttaatt gcggcggcat
    89161 cgtcggcgct ggagtctgcg gccagttctg ccatccgtgt catcaacgcc tgctgctgac
    89221 gcaatgctgc cgccgtggcc agctcttcgc taatcaccgc accaggccac cagctgtttg
    89281 ccagccgcgg ttggcctacc agatcgatta aatgctctgc acccgtcagc gttttggcat
    89341 cactgctgcc attgctgaat accttgacgg ttcccgccgc gaagaccgat gacgccccca
    89401 cactcaaaat caatgcgaca atagtttgtt taatcattgt tttgccgcct tgatgagggt
    89461 ggttttcacc gggaagtaat ccgcgccgag atactgttcc gactggcgaa tttgcccttc
    89521 gctgtcgatc cagtagcggt tatgccagcg cgcctggtcg gtggagactt cttcgtccag
    89581 cacgcgaacc ggggtttcat cgctgccgac tttgacggta tcggtgccat cccatttgaa
    89641 gactgagcgt gcggtggcgt agcgtacctg ctggtactcg gtccagccca tcgtgcgcgt
    89701 ccaggttgca ccatcaacaa tttgtgcggg tttaatcagc gggtcggcgg caaggttatt
    89761 cacttcaatc aggttgtcgc cgccaagcaa agtcttcacc agacgaccat gttgtgtgac
    89821 aagggtggcc tgatcctgag tgacccattt ttgttgtccg tcttcagcga aggccagcac
    89881 cacaaataac tgcggcccgc cattaagctg catgtactgg ctggcgtagg gcatattttg
    89941 aatatcatcg tccgtcagct gtacgcctgg cgtaccgaac agactgtccc acagtgaatt
    90001 gcccagctct ttagtcgtgg ctgaacaggc ctgtaatagc aggcagataa gaatgagtgc
    90061 tggtcgcttc acgactcttc tccagagggg cagaataacc acaccggagt gtggttatgg
    90121 ttaatcccct tcatacttca agcttcagat gcgatgactc gaattatttt ggggatacat
    90181 actttattac tgggtactgg tggtcgtggt tgtggtggtc ccggtattag aaccatcacc
    90241 gccaccggtt gccgcgagcg cgacacccac cgccgagctt acggtgctga cgctggtcgc
    90301 ggaggaacta cccgccgaaa ccgacgttgc tgccgatcct gccgcttcgc ccacctgtac
    90361 aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg gcaaaaatgc catacagaac
    90421 ttttttcata acaatttccc ttcattgaat gaatggagat ttacccaaaa catttcgggc
    90481 gggatcgagt atacacaact aaagcatgcg aattacggcg cggggaatta gtgatggagt
    90541 ttt
//
LOCUS       DAJOTB010000002        90001 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJOTB010000002 DAJOTB010000000
VERSION     DAJOTB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.002"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            286..513
                     /gene="feoA"
                     /locus_tag="OHN28_000079"
     CDS             286..513
                     /gene="feoA"
                     /locus_tag="OHN28_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCQ2295532.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            530..2851
                     /gene="feoB"
                     /locus_tag="OHN28_000080"
     CDS             530..2851
                     /gene="feoB"
                     /locus_tag="OHN28_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCQ2295533.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQIASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2862..3098
                     /gene="feoC"
                     /locus_tag="OHN28_000081"
     CDS             2862..3098
                     /gene="feoC"
                     /locus_tag="OHN28_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCQ2295534.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            3365..3727
                     /locus_tag="OHN28_000082"
                     /pseudo
     CDS             3365..3727
                     /locus_tag="OHN28_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(3756..4526)
                     /gene="bioH"
                     /locus_tag="OHN28_000083"
     CDS             complement(3756..4526)
                     /gene="bioH"
                     /locus_tag="OHN28_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCQ2295535.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            4564..5247
                     /gene="gntX"
                     /locus_tag="OHN28_000084"
     CDS             4564..5247
                     /gene="gntX"
                     /locus_tag="OHN28_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCQ2295536.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5306..5881
                     /gene="nfuA"
                     /locus_tag="OHN28_000085"
     CDS             5306..5881
                     /gene="nfuA"
                     /locus_tag="OHN28_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCQ2295537.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6245..7561
                     /gene="gntT"
                     /locus_tag="OHN28_000086"
     CDS             6245..7561
                     /gene="gntT"
                     /locus_tag="OHN28_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2295538.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(7671..9755)
                     /gene="malQ"
                     /locus_tag="OHN28_000087"
     CDS             complement(7671..9755)
                     /gene="malQ"
                     /locus_tag="OHN28_000087"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCQ2295539.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVIVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(9765..12158)
                     /gene="malP"
                     /locus_tag="OHN28_000088"
     CDS             complement(9765..12158)
                     /gene="malP"
                     /locus_tag="OHN28_000088"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCQ2295540.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWVKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            12781..15486
                     /gene="malT"
                     /locus_tag="OHN28_000089"
     CDS             12781..15486
                     /gene="malT"
                     /locus_tag="OHN28_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCQ2295541.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15529..15948)
                     /locus_tag="OHN28_000090"
                     /pseudo
     CDS             complement(15529..15948)
                     /locus_tag="OHN28_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="catalyzes the conversion of terminal3'-phosphate of
                     RNA to the 2',3'-cyclicphosphodiester; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            15878..16315
                     /locus_tag="OHN28_000091"
     CDS             15878..16315
                     /locus_tag="OHN28_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2295542.1"
                     /translation="MFCSCKEKLPASVAISRSATWRGNFRELSASVTRMATFATSGRI
                     TLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSA
                     AGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(16297..17055)
                     /locus_tag="OHN28_000092"
     CDS             complement(16297..17055)
                     /locus_tag="OHN28_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCQ2295543.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(17072..17902)
                     /gene="glpG"
                     /locus_tag="OHN28_000093"
     CDS             complement(17072..17902)
                     /gene="glpG"
                     /locus_tag="OHN28_000093"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCQ2295544.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17947..18273)
                     /gene="glpE"
                     /locus_tag="OHN28_000094"
     CDS             complement(17947..18273)
                     /gene="glpE"
                     /locus_tag="OHN28_000094"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCQ2295545.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18463..19968
                     /gene="glpD"
                     /locus_tag="OHN28_000095"
     CDS             18463..19968
                     /gene="glpD"
                     /locus_tag="OHN28_000095"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCQ2295546.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(20022..20627)
                     /locus_tag="OHN28_000096"
     CDS             complement(20022..20627)
                     /locus_tag="OHN28_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295547.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(20630..21397)
                     /locus_tag="OHN28_000097"
     CDS             complement(20630..21397)
                     /locus_tag="OHN28_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295548.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSVDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(21500..23008)
                     /locus_tag="OHN28_000098"
     CDS             complement(21500..23008)
                     /locus_tag="OHN28_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2295549.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(23133..25580)
                     /gene="glgP"
                     /locus_tag="OHN28_000099"
     CDS             complement(23133..25580)
                     /gene="glgP"
                     /locus_tag="OHN28_000099"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCQ2295550.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(25599..27032)
                     /gene="glgA"
                     /locus_tag="OHN28_000100"
     CDS             complement(25599..27032)
                     /gene="glgA"
                     /locus_tag="OHN28_000100"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCQ2295551.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(27032..28327)
                     /gene="glgC"
                     /locus_tag="OHN28_000101"
     CDS             complement(27032..28327)
                     /gene="glgC"
                     /locus_tag="OHN28_000101"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCQ2295552.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(28345..30318)
                     /gene="glgX"
                     /locus_tag="OHN28_000102"
     CDS             complement(28345..30318)
                     /gene="glgX"
                     /locus_tag="OHN28_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCQ2295553.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(30315..32501)
                     /gene="glgB"
                     /locus_tag="OHN28_000103"
     CDS             complement(30315..32501)
                     /gene="glgB"
                     /locus_tag="OHN28_000103"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCQ2295554.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(32774..33877)
                     /gene="asd"
                     /locus_tag="OHN28_000104"
     CDS             complement(32774..33877)
                     /gene="asd"
                     /locus_tag="OHN28_000104"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2295555.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            34069..34662
                     /gene="yhgN"
                     /locus_tag="OHN28_000105"
     CDS             34069..34662
                     /gene="yhgN"
                     /locus_tag="OHN28_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCQ2295556.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(34708..36003)
                     /locus_tag="OHN28_000106"
     CDS             complement(34708..36003)
                     /locus_tag="OHN28_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCQ2295557.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(35910..38006)
                     /locus_tag="OHN28_000107"
     CDS             complement(35910..38006)
                     /locus_tag="OHN28_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HCQ2295558.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(38147..39487)
                     /gene="gntU"
                     /locus_tag="OHN28_000108"
     CDS             complement(38147..39487)
                     /gene="gntU"
                     /locus_tag="OHN28_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2295559.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(39491..40018)
                     /gene="gntK"
                     /locus_tag="OHN28_000109"
     CDS             complement(39491..40018)
                     /gene="gntK"
                     /locus_tag="OHN28_000109"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCQ2295560.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(40157..41152)
                     /gene="gntR"
                     /locus_tag="OHN28_000110"
     CDS             complement(40157..41152)
                     /gene="gntR"
                     /locus_tag="OHN28_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCQ2295561.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(41245..41940)
                     /gene="yhhW"
                     /locus_tag="OHN28_000111"
     CDS             complement(41245..41940)
                     /gene="yhhW"
                     /locus_tag="OHN28_000111"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCQ2295562.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(42063..43100)
                     /locus_tag="OHN28_000112"
     CDS             complement(42063..43100)
                     /locus_tag="OHN28_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2295563.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            43434..43922
                     /gene="yhhY"
                     /locus_tag="OHN28_000113"
     CDS             43434..43922
                     /gene="yhhY"
                     /locus_tag="OHN28_000113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2295564.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(43983..45716)
                     /gene="ggt"
                     /locus_tag="OHN28_000114"
     CDS             complement(43983..45716)
                     /gene="ggt"
                     /locus_tag="OHN28_000114"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCQ2295565.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            45836..46276
                     /locus_tag="OHN28_000115"
     CDS             45836..46276
                     /locus_tag="OHN28_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCQ2295566.1"
                     /translation="MKRLLLLTVLLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(46263..47006)
                     /gene="ugpQ"
                     /locus_tag="OHN28_000116"
     CDS             complement(46263..47006)
                     /gene="ugpQ"
                     /locus_tag="OHN28_000116"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2295567.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(47003..48073)
                     /gene="ugpC"
                     /locus_tag="OHN28_000117"
     CDS             complement(47003..48073)
                     /gene="ugpC"
                     /locus_tag="OHN28_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCQ2295568.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(48075..48920)
                     /gene="ugpE"
                     /locus_tag="OHN28_000118"
     CDS             complement(48075..48920)
                     /gene="ugpE"
                     /locus_tag="OHN28_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCQ2295569.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(48917..49804)
                     /gene="ugpA"
                     /locus_tag="OHN28_000119"
     CDS             complement(48917..49804)
                     /gene="ugpA"
                     /locus_tag="OHN28_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCQ2295570.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(49902..51218)
                     /gene="ugpB"
                     /locus_tag="OHN28_000120"
     CDS             complement(49902..51218)
                     /gene="ugpB"
                     /locus_tag="OHN28_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCQ2295571.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEARIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(51615..52328)
                     /gene="livF"
                     /locus_tag="OHN28_000121"
     CDS             complement(51615..52328)
                     /gene="livF"
                     /locus_tag="OHN28_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCQ2295572.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(52330..53097)
                     /gene="livG"
                     /locus_tag="OHN28_000122"
     CDS             complement(52330..53097)
                     /gene="livG"
                     /locus_tag="OHN28_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCQ2295573.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(53094..54371)
                     /gene="livM"
                     /locus_tag="OHN28_000123"
     CDS             complement(53094..54371)
                     /gene="livM"
                     /locus_tag="OHN28_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCQ2295574.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(54368..55294)
                     /gene="livH"
                     /locus_tag="OHN28_000124"
     CDS             complement(54368..55294)
                     /gene="livH"
                     /locus_tag="OHN28_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCQ2295575.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(55342..56451)
                     /gene="livK"
                     /locus_tag="OHN28_000125"
     CDS             complement(55342..56451)
                     /gene="livK"
                     /locus_tag="OHN28_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCQ2295576.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            56874..57257
                     /gene="panM"
                     /locus_tag="OHN28_000126"
     CDS             56874..57257
                     /gene="panM"
                     /locus_tag="OHN28_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCQ2295577.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
     gene            complement(57254..57781)
                     /locus_tag="OHN28_000127"
     CDS             complement(57254..57781)
                     /locus_tag="OHN28_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2295578.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(57788..58054)
                     /locus_tag="OHN28_000128"
     CDS             complement(57788..58054)
                     /locus_tag="OHN28_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCQ2295579.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(58203..59306)
                     /gene="livJ"
                     /locus_tag="OHN28_000129"
     CDS             complement(58203..59306)
                     /gene="livJ"
                     /locus_tag="OHN28_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCQ2295580.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(59577..60431)
                     /gene="rpoH"
                     /locus_tag="OHN28_000130"
     CDS             complement(59577..60431)
                     /gene="rpoH"
                     /locus_tag="OHN28_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCQ2295581.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(60676..61734)
                     /gene="ftsX"
                     /locus_tag="OHN28_000131"
     CDS             complement(60676..61734)
                     /gene="ftsX"
                     /locus_tag="OHN28_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCQ2295582.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(61727..62395)
                     /gene="ftsE"
                     /locus_tag="OHN28_000132"
     CDS             complement(61727..62395)
                     /gene="ftsE"
                     /locus_tag="OHN28_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCQ2295583.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(62398..63894)
                     /gene="ftsY"
                     /locus_tag="OHN28_000133"
     CDS             complement(62398..63894)
                     /gene="ftsY"
                     /locus_tag="OHN28_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCQ2295584.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            64044..64640
                     /gene="rsmD"
                     /locus_tag="OHN28_000134"
     CDS             64044..64640
                     /gene="rsmD"
                     /locus_tag="OHN28_000134"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCQ2295585.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            64630..64899
                     /locus_tag="OHN28_000135"
     CDS             64630..64899
                     /locus_tag="OHN28_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCQ2295586.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(64902..65261)
                     /locus_tag="OHN28_000136"
     CDS             complement(64902..65261)
                     /locus_tag="OHN28_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCQ2295587.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            65402..66028
                     /locus_tag="OHN28_000137"
     CDS             65402..66028
                     /locus_tag="OHN28_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCQ2295588.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            66102..68300
                     /gene="zntA"
                     /locus_tag="OHN28_000138"
     CDS             66102..68300
                     /gene="zntA"
                     /locus_tag="OHN28_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCQ2295589.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAATGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLSNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(68402..68647)
                     /gene="tusA"
                     /locus_tag="OHN28_000139"
     CDS             complement(68402..68647)
                     /gene="tusA"
                     /locus_tag="OHN28_000139"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCQ2295590.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            68857..69522
                     /locus_tag="OHN28_000140"
     CDS             68857..69522
                     /locus_tag="OHN28_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCQ2295591.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            69595..70152
                     /gene="dcrB"
                     /locus_tag="OHN28_000141"
     CDS             69595..70152
                     /gene="dcrB"
                     /locus_tag="OHN28_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCQ2295592.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(70156..71406)
                     /locus_tag="OHN28_000142"
     CDS             complement(70156..71406)
                     /locus_tag="OHN28_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2295593.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            71505..72554
                     /locus_tag="OHN28_000143"
     CDS             71505..72554
                     /locus_tag="OHN28_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2295594.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            72609..73196
                     /gene="acpT"
                     /locus_tag="OHN28_000144"
     CDS             72609..73196
                     /gene="acpT"
                     /locus_tag="OHN28_000144"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCQ2295595.1"
                     /translation="MYRIVLGKVSTLSAAPLPLGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            73307..74881
                     /gene="nikA"
                     /locus_tag="OHN28_000145"
     CDS             73307..74881
                     /gene="nikA"
                     /locus_tag="OHN28_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCQ2295596.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            74881..75825
                     /gene="nikB"
                     /locus_tag="OHN28_000146"
     CDS             74881..75825
                     /gene="nikB"
                     /locus_tag="OHN28_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCQ2295597.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            75822..76655
                     /gene="nikC"
                     /locus_tag="OHN28_000147"
     CDS             75822..76655
                     /gene="nikC"
                     /locus_tag="OHN28_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCQ2295598.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            76655..77419
                     /gene="nikD"
                     /locus_tag="OHN28_000148"
     CDS             76655..77419
                     /gene="nikD"
                     /locus_tag="OHN28_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCQ2295599.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            77416..78222
                     /gene="nikE"
                     /locus_tag="OHN28_000149"
     CDS             77416..78222
                     /gene="nikE"
                     /locus_tag="OHN28_000149"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCQ2295600.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARTSEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            78228..78629
                     /gene="nikR"
                     /locus_tag="OHN28_000150"
     CDS             78228..78629
                     /gene="nikR"
                     /locus_tag="OHN28_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCQ2295601.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            78828..79574
                     /locus_tag="OHN28_000151"
     CDS             78828..79574
                     /locus_tag="OHN28_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2295602.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            79599..80072
                     /locus_tag="OHN28_000152"
     CDS             79599..80072
                     /locus_tag="OHN28_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2295603.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            80069..80350
                     /locus_tag="OHN28_000153"
     CDS             80069..80350
                     /locus_tag="OHN28_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCQ2295604.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            80427..81785
                     /locus_tag="OHN28_000154"
     CDS             80427..81785
                     /locus_tag="OHN28_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCQ2295605.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSSGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            81778..83286
                     /locus_tag="OHN28_000155"
     CDS             81778..83286
                     /locus_tag="OHN28_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCQ2295606.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVD"
     gene            83276..83545
                     /locus_tag="OHN28_000156"
     CDS             83276..83545
                     /locus_tag="OHN28_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCQ2295607.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKCSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            83577..84428
                     /locus_tag="OHN28_000157"
     CDS             83577..84428
                     /locus_tag="OHN28_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HCQ2295608.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(84537..85661)
                     /locus_tag="OHN28_000158"
     CDS             complement(84537..85661)
                     /locus_tag="OHN28_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2295609.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(85661..88396)
                     /gene="rbbA"
                     /locus_tag="OHN28_000159"
     CDS             complement(85661..88396)
                     /gene="rbbA"
                     /locus_tag="OHN28_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCQ2295610.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYISLGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(88393..89460)
                     /locus_tag="OHN28_000160"
     CDS             complement(88393..89460)
                     /locus_tag="OHN28_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2295611.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
BASE COUNT        21409 a        24521 c        22766 g        21305 t
ORIGIN      
        1 cattaacaac tgaaacctta attaaacatt aaccagccct aataattcgt catgcgaatt
       61 atattttcgc tgcgatataa gcttgagcca tatcaacatt gagtcagatt attattcaaa
      121 ccaacattca cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact
      181 gttgattatt taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg
      241 ccccatgagg tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca
      301 gatactgcgt ggaaaattac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa
      361 ctgctttctc ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc
      421 ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg
      481 gccttattag aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt
      541 aaccattggc ttaattggta atccaaattc tggcaagaca acgttattta accagcttac
      601 tggctcacgt cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca
      661 attctccacc accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac
      721 caccatctca tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg
      781 cgacgcagac ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct
      841 gacgctgcaa ctgttggaac tcggcattcc ctgcatcgtg gcgctgaaca tgctcgacat
      901 tgccgagaag caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc
      961 ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga
     1021 tcgctataaa gctaacgaga atgtggaact ggtgcattac gctcagccgc tgctcaacga
     1081 agcagattca ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct
     1141 gggcctgcaa atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca
     1201 gcatctggat gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat
     1261 tgccgatgcg cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac
     1321 ggcagaaccc agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg
     1381 tctgccgatt ttcctctttg tgatgtacct gatgttcttg ctggctatca acatcggcgg
     1441 ggcgttacag ccgctgtttg acgtcggctc cgtagcactc tttgtgcatg gtattcagtg
     1501 gattggctac acgctccact tcccggactg gctgactatc ttcctcgccc aaggcctggg
     1561 cggcggtatt aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct
     1621 ctccttcctt gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgtctgat
     1681 gcaggcgctg ggcttgccgg ggaaatcctt tgtaccgctg atcgttggtt tcggttgtaa
     1741 cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat
     1801 catgatggca ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc
     1861 tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tgggtatcgt
     1921 aatggcggtg ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc
     1981 gtttgtcatg gagctgccgg tctaccatgt accccacgtt aaaagcctga ttatccagac
     2041 ctggcagcgt ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat
     2101 tttcctgagc gccttcaaca gcttctcatt gagcgggaaa atcgtcgata acatcaacga
     2161 ctcggcgctg gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtaca
     2221 tgaagataac tggcaggcaa cggttggcct gtttacaggt gccatggcga aagaagtggt
     2281 ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc
     2341 ggcggaattt aacctcggtg aagagctgtt cagtgcggta gatgaaacct ggcagagcct
     2401 gaaagacacc ttcagcctta gcgtactgat gaaccccatt gaagcaagca aaggcgacgg
     2461 cgaaatgggt accggagcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc
     2521 atacagctac ctgattttcg tcctgctgta tgtaccatgt atctcggtga tgggagccat
     2581 cgcccgtgaa tcaagccgtg gctggatggg cttctccatt ctgtgggggc tgaatatcgc
     2641 ttactcgctg gcaacattgt tctatcagat cgccagctac agccagcatc caacctacag
     2701 cctggtatgc attctggcgg ttattctgtt taacatcgtg gttatcggtt tgctgcgtcg
     2761 cgcgcgtagc cgggtggata tcgaactgct ggcaacgcgc aaatcggtaa gcagttgctg
     2821 tgcggccagt accactggcg attgccacta aggacacgac aatggcttcg ctcattcaag
     2881 tgcgtgattt actggcatta cggggccgta tggaagcggc ccagattagc cagacactga
     2941 acactccgca gccaatgatt aacgccatgc tgaaacagct agaaagtatg ggaaaagcag
     3001 tgcggattca ggaagaacct gacggctgtc tttccggcag ttgtaaaagc tgcccggaag
     3061 gaaaagcctg tctgcacgaa tggtgggcgt tgcgttaacc ttgtttcact gcctgttttg
     3121 aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat agcagcgcga
     3181 cgtaagttcc tctgaaagcg tctcgctggg atgaaaactc gctaatgcac aggtgtggag
     3241 tggcgcgtag agtcgcggca ttcaaataac aggtgaagga acgccatgag caaaaagcag
     3301 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga gacggcacgc
     3361 gagcatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgataggact
     3421 ggtagagcaa atggcctgct tattaagtag tggatacgca aatgacagac aaatcaaagg
     3481 gctgtttaat tacatactgc aaaccggcga cgcggtacgt tttaacgatt ttatcgatgg
     3541 tgtggccgaa cgttcaccaa aacacaagga gagtttaatg actattgcgg aaagattgcg
     3601 gcaggaaggg gaacaatcca aagccttgca tatagcaaaa ataatgcttg aatccggagt
     3661 ccctcttgca gacatcatgc gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag
     3721 ccagtaaagt tctgtcccgc catttcgaaa gccacctaca ccctctgctt caacgccacc
     3781 agcaggtgac aaaactcgtc cggatgtgaa ataaacggcg catgggccgc tttggcgaag
     3841 atatatgatt cgctgtgagg ccaaagttta tccagcatcg gcaccacttt gcgcggcacc
     3901 agaccgtcga gatagccata caatcgcaaa aacggcatgg gcacgttttg cagcggctga
     3961 cggagatcga ccgttttcag gatttccagt ccgccattga gtacgtcaac ctccggcatc
     4021 ggtaacgcca gaacggtttt cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtaccc
     4081 atggtttgta acgccaggaa ccgctccact gtacgctgaa aatcatcact gagttgctgc
     4141 tgaaatcccg ccagcacgtc cggttttatc cccggccact cgtcgcgagc actaaaacaa
     4201 ggtgacgacg ccacggtgac cagcgcctga acacgctcgg gatgggttaa cgcaatctgg
     4261 cttgccacca gcccgcccag actccagcct aaccaaatgg ctttatctgg tgcctgtcgc
     4321 agcacgactt cggccatatc agcaagtgat agcgcaccaa atccccggct acgcccgaag
     4381 ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc
     4441 cacacttcgg cattcagtcc ccatccgtgc agcagcacaa gatgaacatt cccctgacct
     4501 ttggtctgcc accagatgtt attcatccgc tattgttctc tttttactta caaggatgaa
     4561 catatgctaa cagtaccggg attatgctgg ctatgccgaa tgccgctggc gttaggtcat
     4621 tgggggattt gttctgtctg ctctcgcgcc gcccgtacag ataaaacgtt atgcccacaa
     4681 tgtggattac ccgccacaca ctctcatctt ccctgcggtc gctgcctgca aaaaccgctg
     4741 ccctggcaaa gactgctcac ggtcgccgac tatgcgccgc cattaagtcc gctcattcac
     4801 cagcttaagt tttcccggcg cagtgaaatc gccagcgccc tgtcacgtct gttactattg
     4861 gaagtcttac acgctcgtcg caccaccggc ttgcaactgc ctgatcgcat catcagcgtt
     4921 ccgttatggc agcgccgtca ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag
     4981 ccgttatcac gctggctaca ctgccaatgg gatagcgaag ccgtcacacg aacacgggcc
     5041 accgcgaccc agcattttct taacgcccgg ctacgcaaac acaacctgaa aaatgccttt
     5101 cgccttgaat tgcctgtgca aggtcgccat atggtgattg tggatgatgt cgttaccacc
     5161 ggaagtaccg tcgcagagat tgcgcagttg cttttacgca atggtgcggc gactgtccag
     5221 gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg ggcgtattat aaccaactaa
     5281 aatagtcaac tattaggcca ttactatgat ccgtatttcc gatgctgcac aagcgcactt
     5341 tgccaaactg ctggcaaacc aggaagaagg gacacaaatc cgcgtatttg tgattaaccc
     5401 tggcacgcct aacgctgaat gtggcgtttc ttattgtccg ccggacgctg tggaagccac
     5461 cgacactgcc ctgaaatttg acctgctgac cgcgtatgtt gatgagttaa gcgctcctta
     5521 cctggaagat gcagagatcg attttgttac cgaccagttg ggttcgcagc tgacgctgaa
     5581 agcgcctaac gccaaaatgc gtaaagtggc tgacgatgcg ccgctgatgg agcgcgtgga
     5641 gtatatgctg caatcacaga tcaacccaca gcttgctggt cacggtggtc gcgtttcgct
     5701 gatggaaatc accgaagacg gttacgccat tttgcaattt ggcggtggct gtaacggttg
     5761 ttccatggtc gacgtcaccc tgaaagaggg gatcgagaag cagttgctga acgaattccc
     5821 ggagctgaaa ggtgtacgcg atctcaccga acaccagcgc ggcgagcact cttactacta
     5881 agttctctcc ccttttgtcg attgcccgat gtgacgctaa agcgtcttat cgggcctgta
     5941 cgttgcacga gagtctgacg ttagcgcaga attatctcag caaatccaat ctcttcgttt
     6001 ccccacgata atatgaccaa cctctcataa tttaaattta ccccgctctg gtgattctca
     6061 agcacgtaat gttacccgta tcattctcat gggcaccaaa cacactcata acatctgact
     6121 acactaactc gttttagggg tcatcagccg tggtgccgta acatctctgt tcccagttgg
     6181 gacaaccagg attttccgtc agaaacctga cgttacccat aacaaattaa aggccaggta
     6241 aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttattactcc tgatgatccg
     6301 cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgctgttg gattaatgca
     6361 agggatgccg ctggataaag ttattggctc catcaaagct ggtgtcggcg gaacgctcgg
     6421 tagccttgcc ctgatcatgg gttttggtgc gatgctgggc aaaatgctgg cagactgcgg
     6481 tggtgcacaa cgtatcgcta ccacgctgat tgccaaattt ggtaaaaaac acatccagtg
     6541 ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggtttcgt
     6601 gctgatgctg ccgctggtgt ttaccatcgc ggcttccgcg aatattccac tgctgtatgt
     6661 tggtgtacca atggcggctg cactgtctgt gacccacggc ttcctgccac cgcatccggg
     6721 tccgactgcg attgccacca ttttcaatgc cgatatgggt aaaactctgc tgtacggtac
     6781 tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgttgaaagg
     6841 tatcgataag ccgatcccgg aaggtctgta cagcgcgaaa accttcagcg aagaagagat
     6901 gccgagcttt ggcgtcagcg tctggacctc tctggtgccg gtggttctga tggcgatgcg
     6961 tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct
     7021 cggtgacccg gtaatggcta cgctgattgc cgtgctgatc gcgatgttca cctttggtct
     7081 gaaccgtggc cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat
     7141 tgcgatgatg ctgttgatta tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag
     7201 cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat
     7261 ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg tcgcggcaat
     7321 cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cggagctgat
     7381 ggttattgcg gttggttccg gtagtgtgat tttctctcac gtgaacgatc cgggcttctg
     7441 gctgttcaaa gagtacttta acctgactat cggcgagacc atcaaatcct ggtcgatgct
     7501 ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg
     7561 ataatactga tggccggatg cggcgtgaac gccttatccg gcctacgatt cggcattaat
     7621 tagtaggccg gtaagacgcg tcaagcgtcg catccggcat caaccgcact ctacttcttc
     7681 ttcgctgccg ctctgcgccg tctgtccaaa tccttcagca acttgttcac accatcatcg
     7741 gcaaacatcg actcaagcgt tgcggaaagt ttacgtcgcc agtttttata ctggtaactg
     7801 gtgccgggaa tatttaccgg atctgccata tccagccagt cttccggctg gagccctaac
     7861 agggcgctgt tgctatcggc gatgtagcgc tgtaagccac gattcagcgt cggtgtcatc
     7921 gacatcaacg atgccttatg cccggcacgt tttggcaggc aaccatattt atgcagagca
     7981 tccagcagcc cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca
     8041 tccggataca gccccagcgt tttgcccagc gttaaatccc cactctccca gtagccgcgt
     8101 agcgtaggca ggtcgtgtgt cgccgcaacc gccatcgact gctctgggta cgctttcggt
     8161 gcgcggaatg tcttctcgtg gtcgttttcg aaatagagca ctttgtagga gtacacaccg
     8221 ctgctgcgca gcttaccgac aatctctacc ggtacggtac cgagatcttc accaatcacc
     8281 atacagcgat gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag
     8341 tgaacatacg cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc
     8401 gacatcacgt gatcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg
     8461 ataaacggtt cataggcacg cgcggtgatg atatgcgggt ccattggcgg caatccccag
     8521 ttctgcccca acgggccgag gatatccggc ggcgcgccaa ccgacgcttt caggcaatac
     8581 agttcacggt cgcaccaggt ttctgcccca ccttctgcta caccaacggc cagatcacga
     8641 tacaagccaa tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg
     8701 taagccagcc actgcaacca gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac
     8761 tggcgcactt ctggcgaatc cacgttctga tacatctctg gccatgctgg ccagccccag
     8821 cgcatttcgt cctctttcac ttgctgggca tgtagcgcat caaaggctgc ctgccagaac
     8881 aggctgtcgc cctgctctgc aacaaactgg cgaaacgcgg tcatctgctc atcatcacgt
     8941 tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag
     9001 taatcaaccc agtcggcatc ccgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc
     9061 caccaggcct gagcctcttc gctaagatgg aaatcttcaa ctgcgttaac gtcgatataa
     9121 atcacattca gccaacggcg agaagacggg ctgtacgggc tggcactttc cggatttgcc
     9181 agatagagcg catgaatcgg gttcagacca ataaacgacc cgccacgttt tgccacatcc
     9241 accagcatcg ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc
     9301 gtataaagct gaacgcaggc accccacagc ttttgtttat tcagcaacgc ctgcggttcg
     9361 taacagcgtt tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtaagc
     9421 gtcagcgtgt gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtc
     9481 acatggcctt tgtactgctt tccttcttcg gtggtcagca gccagctata ttcgccgctg
     9541 ccctccacca ccatcggcat ttttttgccg ctggtataaa ctatgacatt cgggactggc
     9601 gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt
     9661 tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc
     9721 gccagcgcgg cattatccag acgtttgctt tccatcgaac ttccttagcg ttttgcctgc
     9781 cagatacgag cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg
     9841 gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc
     9901 ttttgtgcct ccacataagc ggcgaaatct gccatcacca gatacggatc gccgccctgt
     9961 ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg
    10021 ctttccagct ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg
    10081 tcgtagcctt tggccagaat tgccttcact tgttccacgg tatgaccaaa aataaagata
    10141 ttttcttcgc cgactttctc agcgatttca acgttcgcac catccagcgt accgacagta
    10201 agcgcaccat tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc
    10261 gaaatttgtt cggagatatc cgccgccggg atcagttttt ccgccgccga aacacaataa
    10321 tccggcaaga acaccacctt caacttgtcg ccaaccagcg gatcgttgtt gatcacgtca
    10381 gccactttgt tgatcgcaaa gataatattt ttcgccaggt agtagcccgg tgctgctttc
    10441 gcgccgaaga ggaagacgcg cggtacgcgg tcagactgcg ggttttcacg aatttctttg
    10501 tacaacgcca gaatatgcag cagattcaga tgctggcgtt tgtactcatg cagacgtttg
    10561 atctgaatat cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac
    10621 tctgccagac ggactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca
    10681 tcatcagcga atttttccag attgatcagt tgatcgagat cgttagccca ctcttttttc
    10741 agtgatttat ccaacagagc cgccagtgcc gggttgcact gtttgaccca gcgacgtggg
    10801 gtaataccgt tggtgacatt atggaatttg ttcggccata gctggtgata ttccgggaac
    10861 agatctttca ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca
    10921 accacacaca ggttcgccat atgcacttgt ttgtcgtgca ccaccgccag tttggcccac
    10981 actttttcat cgcccggcca ggttttctcc actagcgttt taaagcgagt attaatttcg
    11041 ttaataatct gcatgtggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc
    11101 agcgcttctg gcatcagggt atggttggtg taagcgaaag ttttgctggt aatggcccag
    11161 gcgtcatccc agctcatctg gtgctcatcg atcagcacgc gcagcagttc tggaatcgcg
    11221 attgttggat gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcaattta
    11281 cgccccgcca gatgatggcg acgcaaaata tccgctaccg agcaggcaca ctggaagtat
    11341 tgctgcatca ggcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg
    11401 gtcagttttt ctgcgttgat gccctgctgt tcggcacgca ggaaatcacc atcgttaaat
    11461 ttagtcagat caaacggatg cgcatgcgtc gcctgccaca gacgcagcgg ctgcgccacg
    11521 ccattacgat agccgacaac ggggagatcc cacgcttgac cggtaatggt aaattccggc
    11581 tcccagcgtc cgtctttcgt cactttaccg ccaatcccta cctgcacatc cagtgcttcg
    11641 ttgtggcgga accacgggta gttaccgcga tgccagtcat ccggcgcttc aacctgtttg
    11701 ccatcgacaa aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca
    11761 gactgaccga cagttgccat tgagtcgaga aagcacgccg ccagacgtcc cagaccaccg
    11821 ttccccagcg ccgggtcgat ctcttcttcc aacaggtcag tcaggttgat gtcataagcc
    11881 ttcaacgaat cctgtacatc ctgataccag ccgagattca acagattgtt gcccgtcagg
    11941 cgaccaatca aaaactccat tgagatgtag ttaacatgcc gctgattcgc caccggtttg
    12001 gcaaatggct gagcacgcag catttcggcc agtgcttcgc tcactgccag ccaccactga
    12061 cgaggggtca tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcgct
    12121 tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat
    12181 tttcaacaca acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg
    12241 gggattaggc agggaggagt tgcggggatg agcaaggaaa tgtgatctcg accacttaaa
    12301 gctagtgcaa tccacaggat tagcagcaaa tcaatgccat accgcgcaga aaatctgtat
    12361 ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta
    12421 agtaaaggag tgaaacagtt tcacaagtaa aatatcacgt gtgctccatc tcattcttaa
    12481 tagatttatt aagatcatct ttttagatgg cactttcatc agaaatgaag aagaaaccct
    12541 tgcttaaatg aaactgatga acataaggaa aataccgtac aacccagtat tcacgctgga
    12601 tcagcgtcgt tttaggtgag ttgttaataa acatttggaa ttgtgacaca gtgcaaattc
    12661 agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca
    12721 ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact
    12781 atgctgattc cgtcaaaatt aagtcgcccg gttcgactcg accataccgt ggttcgtgag
    12841 cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct
    12901 gcgggctacg gaaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc
    12961 tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc
    13021 gccgtgcaac aggcaaccaa cggtcactgc gcgatatgtg agacgatggc gcaaaaacgg
    13081 caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat
    13141 agcccacttt atctggtcat cgatgactat catctgatca ctaatcctgt gatccacgag
    13201 tcaatgcgct tctttattcg ccatcaacca gaaaatctca cccttgtggt gttgtcacgc
    13261 aaccttccgc aactgggcat tgccaatctg cgtgttcgtg accagctgct ggaaattggc
    13321 agtcagcaac tggcatttac ccatcaggaa gcgaaacagt tttttgattg ccgtctgtca
    13381 tcgccgattg aagctgcaga aagcagtcgg atttgtgatg atgtttccgg ttgggcgacg
    13441 gcactgcagc taatcgccct ctccgcccgg cagaatactc actcagccca taagtcggca
    13501 cgccgtctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggtttta
    13561 gataacgtcg atctcgcaac acgccatttt ctgttgaaaa gcgccatttt gcgctcaatg
    13621 aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg cctcgaagag
    13681 attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg gttttgctat
    13741 cacccactgt ttggtaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg
    13801 ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc cagcgaagcg
    13861 attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac
    13921 gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg
    13981 ccgtgggaca gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg gctgatgcaa
    14041 agccagcatc gctacggcga agttaacacc ctgctggccc gcgctgaaca tgaaatcaag
    14101 gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt
    14161 aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga agagctgccg
    14221 ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt gttgcactgc
    14281 aaaggcgaat tgacccgctc actggcgtta atgcagcaaa ccgaacagat ggcacgccag
    14341 cacgatgtct ggcactacgc tttgtggagt ttaatccagc aaagtgaaat tctgtttgcc
    14401 caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag
    14461 cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcattcgtgc gcaactgtta
    14521 tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gcagcgggat tgaagtcttg
    14581 tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg
    14641 gcccgtggtg atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg
    14701 aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg
    14761 caaatgacca gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccagag
    14821 tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgtgc acaaatcttg
    14881 ctgggcgagt ttgaatctgc ggaaattgtc ctcgaagaac tcaatgaaaa tgcccgcagt
    14941 ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact gtactggcag
    15001 gccggacgta aaagtgacgc tcagcgtgtg ttgctggacg cattaaaact ggcgaatcgt
    15061 accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag
    15121 cttattcaac tcaatacgct gccggagctg gaacagcatc gcgcacagcg tattctgcga
    15181 gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt
    15241 ctgctaaatc atccggaagt gccagagctg atccgcacca gcccgttgac ccaacgtgaa
    15301 tggcaggtcc tggggctgat ttactccggt tacagcaatg agcaaattgc aggcgagctg
    15361 gaagtggcgg caaccaccat caaaacgcat atccgtaatc tgtaccaaaa actcggtgtc
    15421 gcccaccgtc aggccgcggt acaacacgcg cagaaactgc tgaagatgat gggatatggg
    15481 gtgtgagttt agccggataa tgcgtcggat ccggcttaca tctttacatc attcaatgct
    15541 aacccgcgtt acgccatctg tttctatcaa accaaaccgc accggcaaga aacgctccac
    15601 cacggcgata ttggtcagca gatgacatga gggatgggcg accgtaaatt ctcccgcgcc
    15661 cgccagcgcc atcggtagca ccagttggtc ggcgagatat tcccccaccg ccgccgggct
    15721 tgccaggtag cgtttcacct ctttcaccaa ctgtgccgcg accacctcgg cactgacgcg
    15781 cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgagac
    15841 ggtattaccc ggcccctggt cgcgcggcag gttatgaata ttctgttcat gcaaggaaaa
    15901 actacccgcc agtgtagcga tttcacgctc agcaacatgg cgcggtaact ttcgcgaact
    15961 ttctgccagc gtcacgcgga tggccacctt tgccactagc ggacgcatca cactggatac
    16021 agtggaagat gagatcaacc gtctgcgcta taactggcag gagagtcgcc cctccacgct
    16081 tacggcgttg ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta tgcaactgga
    16141 acacgttatc gctatctgcc gccaggcaaa gtcgctttct gctgccggac gtgaactgtt
    16201 tgacatttcg cgccagggta aagccagcgt caatgacgcg gatcggctac gcaaatacct
    16261 ggcgcgtttt ggtctgacat gggaagcaat gcaggatcag cacagctcca gttgaatatg
    16321 atggtccttc agcacctgca tcacgctcgc aggcggcatc gtgtcggtgt agacagcatc
    16381 gaccatgctg atgctaccca tattgaccat tgcgttacgg ccaaattttg agtggtcgac
    16441 aaccagcata acgtggcgcg agttctcaat gatggcgcgt ttggtgcgaa cttcgtggta
    16501 atcgaactcc agtagcgagc cgtcgctatc gatgccgctt atccccagaa tgccgaaatc
    16561 aaggcggaac tgggagataa aatcgagcgt tgcttcgcca atgatcccgc catcgcggct
    16621 gcgtaattcg ccaccggcaa gaatgatacg gaaatcctct tttaccatca gcgtgttagc
    16681 gacgttgaga ttgttggtca caatgcgtaa attgctgtga ttaagcagcg cgtgcgccac
    16741 cgcttccggc gtggtgccga tatcgataaa cagcgtcgag ccattgggaa tttgctccgc
    16801 cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg gccttgcgat cgtgccacgg
    16861 cgtgttaacc gaactggaag gcagcgccgc accgccatga tggcgcagga tcaggttttg
    16921 ctctgccagc tcgttgagat cgcggcgaat agtctgcggg ctaacggaga aatgctctac
    16981 cagctcttcg gtgctgacat aaccctgctg tttaaccagt tcgataatac cgttgtgacg
    17041 ttgtgtttgt ttcattgata aatccctgga attattttcg ttttcgcgca ttgagcgaat
    17101 caacaaaagc catcgctaaa cccacggcta acccggcgat atgtgctccg ttcgccatcg
    17161 acatcccaaa caaatcaaac catccggcga caatccagat caacgcaaag ataattaacc
    17221 cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc acgtagccag acgtagccca
    17281 tcagcgcata caccacgcca gaaagcccgc caaaccacgg cccgctgaat ttttgctgca
    17341 catagccgct taacagggcg ctaatgagag taatgacaat tagcttaccg ctaccgaggc
    17401 gtttttccac cgcaccgccg agataccacc accagagcag gttaaagagg atatgcatca
    17461 gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc agcgtcggat
    17521 cgaatggcca ggccagccat aacatcactt cctgatcgcc gagaatttgc atggcgataa
    17581 acaccaccac gcaggcgatc atcatcaccc aggttaccgg acctgcgcgt tcacgcaagg
    17641 cggcaaagaa aggataacgg cgataatgca ggccactgtc ggtatgaccg gactgccaac
    17701 tggcggcgag ataacgtgga tctgccgggt tttcgagaaa acgagccagc tccgcccgca
    17761 cgcgttcggc ctgggactca tccgccagcc agacatcgct ttggttatgt tgttgaatcg
    17821 tgaggataac accctgtgtc gccatgtaat caacaaacgc ctgcgccact cgggggttag
    17881 caaaagaggt aatcatcaac atcgttgctg tcgcttattc cacacaaaag gggacagtat
    17941 aaagcgttac gcgccgtacg ccacctctgc gggaaactgg cgctgccagg cttcaaaacc
    18001 gccatcaatg ctgtagacca catcgtagcc ctgttgcagc agatactgcg ccgcgccttt
    18061 gctgctattg ccgtgataac acatcaccat caccggtgtg tcaaagtcgt tatcacgcat
    18121 aaacgcgccc agtgtgtcgt tagttaaatg gaaagcctgg gtcgcatgcc ccattgcgaa
    18181 actctgtgga tcgcgaatat cgaccagcac cgcctctttt tcctgcaact tctggtgcgc
    18241 gtcggcaacg ttaatacatt cgaactgatc catgcgtctc tctttcttta caaacaagtg
    18301 ggcaaattta ccgcacagtt tacgtcgaag cggcagataa acgccataat gttatacata
    18361 tcactctaaa atgttttttc aatgttaccc aaagcgcgat tctttgctaa tatgttcgat
    18421 aacgaacatt tatgaacttt aacgaaagtg aatgagggca gcatggaaac caaagatctg
    18481 attgtgatag gtggcggcat caatggtgct ggtatcgcgg cagacgccgc tggacgcggt
    18541 ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg cgacctcttc cgccagttca
    18601 aaactcattc acggtggcct gcgctacctt gagcactatg aattccgact ggtcagcgag
    18661 gcgctggctg aacgtgaagt gctactgaaa atggccccgc atatcgcctt cccgatgcgt
    18721 tttcgcctgc cgcatcgtcc gcatctacgc ccggcgtgga tgattcgcat tggtctgttt
    18781 atgtacgatc atctgggcaa gcgcaccagc ctgccgggat caaccggttt gcgttttggc
    18841 gcaaactcag tgttaaaacc ggaaattaag cgcggattcg aatattctga ctgttgggta
    18901 gacgacgccc gtctggtact cgccaacgcc cagatggtgg tacgtaaagg cggtgaagtg
    18961 cttactcgta ctcgtgccac gtctgctcgc cgcgaaaacg gcctgtggat tgtggaagcg
    19021 gaagatatcg ataccggcaa aaaatataca tggcaggcgc gcggcctggt taatgctacc
    19081 ggcccgtggg tgaaacaatt tttcgacgac gggatgcatc tgccttcgcc ttatggcatt
    19141 cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc atacccagaa gcaagcctac
    19201 attctgcaaa acgaagataa acgtattgtc ttcgtgatcc cgtggatgga cgagttttcc
    19261 atcatcggca ccaccgatgt ggaatacaaa ggcgatccaa aagcagtgaa gattgaagag
    19321 agtgaaatca attacctgtt gaaagtgtat aacacgcact ttaaaaagca gttaagccgg
    19381 gacgatatcg tctggaccta ctccggcgtg cgtccgctat gtgatgatga gtccgactcg
    19441 ccgcaggcca ttacccgtga ttacaccctt gatatccatg atgaaaacgg caaagccccg
    19501 ctgctgtcgg tattcggcgg taagctgacc acctaccgca aactggcgga gcacgcgctg
    19561 gaaaaactga cgccatatta tcagggcatt ggtccggcgt ggacgaaaga gagcgtgctg
    19621 ccgggtggtg ccattgaagg tgaccgtgac gattacgccg cccgcctgcg ccgccgttac
    19681 ccgttcctga ccgaatcgct ggcacgtcac tacgcgcgca cttacggcag caacagcgaa
    19741 ctgttgctcg gcaatgcagg ggcgataagc gacctcggtg aagatttcgg tcatgagttc
    19801 tacgaagcgg agctgaaata tctggtcgac cacgaatggg tccgccgcgc cgacgatgcc
    19861 ctgtggcgtc gcacgaaaca aggcatgtgg ctaaatgcgg atcaacaatc tcgtgtgagt
    19921 cagtggctga tggaatatac gcagcagagg ttgtcgctgg cgtcgtaagg atgatgcccg
    19981 gttgcgaagt gcaaccgggc tatttgaatt ccttagacta ttcattgtaa ataattgaat
    20041 aacggcccca gagctaaaat cgccaaactt aagcacgtta cccaatcgcc ccacttcttc
    20101 ctatggcttc tatttttgct acgccactgg caataatatc ccaacgcaaa accaagctgg
    20161 gcactaatcg ggccgacaaa atcagtccac ggctgcggcc aaaaagggga aaaattcagg
    20221 ggaagccaga caaatgtcag aaagaaattt cggatatttt ccagcaacgc tgatttctct
    20281 ggagaaatat aaagaatgaa attcgagaga attaaccaga aatagaccgg agaaaaaacc
    20341 agccagcaat gtagcctgtt ttttaattca ggaggcgttc tgcgggagaa ttgccaaccg
    20401 atgccaccca ttacactgaa aaaaagtgca tattcaacca ataactcaag ttgtaattcc
    20461 tgaagagaaa tacgcacaac caggagcgga aaggagaata ttatttcagc cagaaaagtt
    20521 aacgtgaata taagtacggc taaggcaata accgtcggcc ataccaataa tggtacgata
    20581 acaaaaccga aaattacagg gtaaatataa agtctgattt tattcatctt taatgtcctg
    20641 atgattcgga tgatgcgtca tacatggcac ataaacactc atcaatcatc cactcgtctt
    20701 cccaggcctg atactgtgtg ccctgttcgg actgcttaag cggattctca atcgcctgcc
    20761 gcgcgcgttc tttcaggatg gattgattat tactagccca agtattagtt tgagtggtaa
    20821 tggctttatc gatatcgtcc tgattatctc tgatataacc gtcacgatac gtattcaata
    20881 aacaggcaag agacgctgcc gaccaaaatc cgggaactga aataatggca tcaagcaatg
    20941 gcgaaagagc gatatcggca cggaaaaaca tttccagctt gtactgtaat tcgacagcct
    21001 catccacgga tacccaatca gtcatatact ggcaagcaaa ttgctgcaat atttcccgtt
    21061 catcctcacg ccagttttct ttactggcaa ggttgatacg ccgtagagaa aatacttcgt
    21121 gaatattgtc aaattgcccc tgcgcaacaa actccagtaa acaaggcaga aaataacgaa
    21181 cctcatcgct gttttgcgga tcaggtcctg gactactatt ccaggcattg attaaggaat
    21241 aaggaatcgc tcgcaacgat gtattccgta atgctttttg ttcctgttga ctcagacaat
    21301 attcacaaca aaccacaaat tgctgcggcg ccggataacg acgaaaaata tgatatatct
    21361 gctcaattaa tgcctgcatg gcaggagtgg ggtgcataat taccccctcg cccgctgatc
    21421 gtgcataaac ggctgataga taaaaagggc attcgccgcg ttcaggaaaa tattaatgat
    21481 atccatattg taatcctgct taatgcaacg ttttatccat cggcacatag cccacatctt
    21541 ccaccaaact ctgcccctgc ggcgttaaaa accactccac caatttttgt gtctctgacg
    21601 tcgtattttc ccgcgttacc attaaggcat cgacgatata gggatatttg tcgttgcgaa
    21661 tattttccgc agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcggcat
    21721 tcatttgtgt tgcgtaatag cggaaggtgt agcctattgc gttttttgta ttacggtatt
    21781 cggcaacaac gttaatcatt ccctccatca cgcttgccac ttccgtttcc tgcggcgaga
    21841 tcatgcggac atttttcatg acctgcgata gcatcaccgt ctggctgcca gagtcttccg
    21901 ggcgttgcca ggtctggatc tcctgatcgt caccgccaac cgcgcgccag ttggtaattg
    21961 cgccactgaa aatgtcacgc acctgttgtt cagtcaggga attaaccgga ttatccgcat
    22021 tgacgatgaa aacaaacgct tcacgggcaa atggcgtgta cagcaaagtg acgcccgatt
    22081 tctcagcgcg ttttttttgc ccatcggaag gttgcgccac gaaaataata tcggcatcac
    22141 cgttaacgat tttgttatat gcttcttgcg tacgagagtt atccagataa tcccaaacgt
    22201 gaaaatcctc tggtattaca cttaatgcat aaaatgcaga ggcataaatg gggtacgccg
    22261 ccgtagcgcc atcgagtcgc ggccagtttt gcgtaaattg aatttgtggt ttaccacgca
    22321 gtggcgtcag ttgattatta agtttatcgg gtcgccaggc ccaggtatca atattttcac
    22381 gcacaccgac accgggatta agctgtttat ataaataagc ctgataccca gcgacaaaac
    22441 caagtaagag aagtagaaac aggagtaagt tacggcaacg tagcccggac gtgccggtta
    22501 aaggatgcct tgtcggccag ccgtatccca gcgaaaaact gatatgcgcg ccaacgggta
    22561 taatgactat ccaccagtag tcgccactga ttaatgagag taacaaaaca ccctgccagg
    22621 aaagcataag gtttgccaac cagcccgatc ccatctggcg aaattgataa tctgccagat
    22681 gtagcgcgat tatccagcca accagggtta atagcgcggg taagagaaaa aagcattgtc
    22741 gccaggcggc tatttctctt ttttctggtg cgctatcacc agcccacacg cgaccagaga
    22801 aaaatatgat tatcgccccc agcgtcagca taaagacgtt gagtagaaga taaattgttg
    22861 gcgtaaagag taaattgcaa acctcaagaa tagcggcaag cccggccccc agcatggcaa
    22921 caaccgctgc caaaaattgc gccagtatgg ggatgccaaa aaaagtcatt accagcgagg
    22981 tcaaaatcca ttttccgctt tgcattattc tttccattct tgctgaatgg cgaaattata
    23041 ctccccgata accctcgccc cctctggaca cttttcctaa ataaaggcga aaaaaacggg
    23101 accttttggc cccgttctgt tttttagcga acttataatc tcaccggatc aatatgccaa
    23161 atataatcgg catactcttt gatcgtacgg tcagaagaga agtaacccat attggcaatg
    23221 ttcagcatcg cttttgcggt ccactcttcc tgacgctcgt agagttcgtc aactttatcc
    23281 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
    23341 agcgaatcga ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
    23401 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
    23461 ccctgacggc gcaattcttc cacttcttcc gctgtgttac caaagataaa gatattgtca
    23521 gcaccgacat gatccagcat ctcgacattc gcaccgtcca gcgtaccgat agtcagcgcg
    23581 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc
    23641 tgttcagaca gatcggccgc cggaatgatc aactgcgcca ggctaacact gtagttcggg
    23701 atgaacacga ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg
    23761 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
    23821 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
    23881 gtgatcacgt gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgc
    23941 acatcgaaca gcgctttcgg atttaccacc acattcagct gctgggcgat atactccgcc
    24001 agacgctttt tgttctccag cttcgcctga tgcaccgcgt gattaaccat tgggaaatca
    24061 cagtgttgtt gcagatcatt aagcaggcta aggtcggtgc gccagttgcg gcccaggtgt
    24121 tcgtccaaca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
    24181 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaatagcgat
    24241 tgcaccatca ggttagagtg cagttccgat acaccgttaa ctttgtggct caccacaacc
    24301 gccagccagg ccatacgcac acgacgacca ttggattcat caatgatcga cgcccgtccc
    24361 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
    24421 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcgac tggccaggtt
    24481 tccagcgcct cgctcattag cgtgtggttg gtgtaggaga agacctgaca acacacttca
    24541 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
    24601 gacagcaccg gatgggtatc gttgaggtga atcgcaattt tatccgccag gttatcgtag
    24661 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag
    24721 tattcctgac gcagtcgcag ctcacgcccg gagtaggtgg agtcgtccgg atacagtacg
    24781 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc gccctggttg
    24841 aatttaccga ggttaatttc actactggct tgggcactcc acaaacgcaa cgtgttggtc
    24901 gcgtcggtgt cgtaacctgg gattatctga tcgtaagcta ctcccagaat ctcttcggtt
    24961 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
    25021 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtcaggcgac
    25081 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
    25141 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcga ggaagcaagc cgccagacgg
    25201 cccaggccac cgttaccgag gcccgggtca ttttcttcat caatcagctc ttcgagattt
    25261 aatcccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
    25321 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
    25381 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
    25441 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccggatcctt tccaatcgta
    25501 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
    25561 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
    25621 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
    25681 acaaaccgcc acagtgaagg acgggaccac agtacgaaag cacgtcgaat agcccgtaac
    25741 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
    25801 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
    25861 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
    25921 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
    25981 acgcccacct ggccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
    26041 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagaccagg taaggcttcc
    26101 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
    26161 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
    26221 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
    26281 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
    26341 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttgggctg
    26401 actgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
    26461 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
    26521 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc
    26581 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
    26641 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
    26701 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
    26761 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
    26821 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc
    26881 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
    26941 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
    27001 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
    27061 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
    27121 cttcctctgc gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga
    27181 cgcagcgacg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
    27241 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
    27301 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
    27361 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
    27421 tgcgatcgta catatccagt tccggcacca cagaggccag atcgaggttc gctttccagt
    27481 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
    27541 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
    27601 ctttgccaaa gtcgtggctg gagttctcat cgcgatcgtc ttcttccagc agttcataca
    27661 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgcct ggatcgttcg
    27721 gcattgacgg cgggttagct ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
    27781 ccgccataac gccaaacgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
    27841 aacgtgcgcc tttttcgacg tggtcgataa gcatacgcga gtagtcttgc ttgtagatat
    27901 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
    27961 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
    28021 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctggatgt
    28081 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
    28141 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
    28201 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
    28261 gggcaacaga tttcaatggc agctggcgtg ccaacattaa gtgatcgttc ttctctaaac
    28321 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
    28381 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
    28441 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
    28501 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
    28561 aggttgagca tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt
    28621 ctccatcaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
    28681 accactgctt gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt
    28741 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
    28801 ggagagcaac aacgttgtta acagggcgtg aatgctgtcg cgccgccgtt caaccagatc
    28861 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
    28921 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
    28981 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gatcattacg
    29041 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
    29101 atgcagccag aaacgacggg ctgcatcgcg gaaatgatcg ttccactcgg caaacagcgg
    29161 cgggaaattt cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt
    29221 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacgaaa
    29281 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
    29341 ttctacccaa taacgcaggc aggcgctggc ataatctacc accgccggat gactcaaatt
    29401 gagcgtgttg ccacaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
    29461 gctacggttg tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
    29521 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
    29581 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
    29641 catcgccacc gggttgtaac cccagtagtt actaagcccc atacgttgca gacgtggttc
    29701 actggcaaat tgtgccaccg gcagcagttc cagcgcggta atgcccaact gtttcaaata
    29761 gttgatcatc accggatgcc ccagggcttt ataagtgcca cggatctcga ccgggatctc
    29821 cgggtgcagg tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
    29881 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca atcaccacta cgcatttcgg
    29941 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
    30001 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
    30061 aaagcgatgc ccctcggcag gttgccaggg gccatgaact cgataaccat aacgcaaacc
    30121 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
    30181 gcgatgttcc tggccattgg catcaaagac acacagctct acccgctcgg catgagcgga
    30241 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag tgggtttgcc
    30301 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag aggtggtagc
    30361 gtcaggctca gtgaatgttg acgaccgtgg ctggcaatct catcgctgtg tactgcgccg
    30421 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
    30481 ttacccggct ggttaatacc gaagcgataa tcatgacgcg gcaccggtgt aaagttactg
    30541 gcaacgatga tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
    30601 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
    30661 ttatggtggc ggtaggtgtg gttcagatcg cgcaccagac gctggacacc gtggtgccag
    30721 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
    30781 tgggcaaatt cgttccccat gaacagtagt ttcttgcccg ggaatgccca catccagccg
    30841 tagtaggcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatccg gtcgagaatc
    30901 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
    30961 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
    31021 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
    31081 ccgcccatat cctgtggacg agaaacgcca gggaaatcgg tagactcctc cgccattgtc
    31141 actgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
    31201 tcaagattct cgcgaccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
    31261 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
    31321 atccagtaaa gcgcgttacc gacaaggaag ttactgactt cacggcgacc atagttgtag
    31381 atcagcgtgt tccagtcctg atgatagcct tcgcgcggat cgctgtgttc atacaagttc
    31441 gtgccatcaa attcggcaag cgcaaagtca tcggtcggga agtggcctgg cacccagtcg
    31501 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
    31561 gtaccaaaac ggcgggtcgg tgcatacagg ccggttggct gataacccca actgccatcg
    31621 aagggatgct cgttaatggg cagtagctcg agatgggtaa agcccatcca tttagcataa
    31681 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
    31741 cgccaggagc ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
    31801 ttgcgctctt cagtctgcac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
    31861 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
    31921 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
    31981 attccgctct ctttacgcag gcgcatcgga tgacggcgac cgtcccagta gttgaattgc
    32041 ccaaccaccg agacccgacg ggcgtttgga gcccagacag aaaaacgcgt acctgtgacg
    32101 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgtaggtg agtaccttca
    32161 gataatagcc aggcatccat ttcctggatt agcgggccaa aacggtaagg atcatcaatc
    32221 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
    32281 ggaatgacac cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
    32341 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
    32401 agtcccgcgg tggttttatg catccccagt acggaaaaag gatccgcaaa atggcctgca
    32461 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
    32521 tgtgtcaccc catccaactg aatttttgac ttctggttgt gacatttttt tgacctgaac
    32581 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
    32641 tcgtgtattc cgccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
    32701 gaaacatctg cgcttactcc tgtattacgc actagcaggg gcggcatcgc gccccagatt
    32761 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
    32821 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
    32881 gcagacggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
    32941 tagtgatttc ccgatcgttt ggaacaactt tcgcccacgg attgtgcgca gccagcagtt
    33001 cttccacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
    33061 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    33121 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcaagct
    33181 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    33241 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    33301 ggttcgcgag ttcatcagcc acatggccat acagatggcc catctgggtt aataactcac
    33361 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    33421 caagatcatt ggcgaataaa ccacccagcg acatcaacat caggcttacg gtacagttac
    33481 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    33541 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga tgatgctgcg tcaatccagt
    33601 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    33661 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    33721 tgccagtggt tccgccaaaa gacggcgcag cctgaccaag ctgagaagta gaaaagaaga
    33781 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    33841 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    33901 tgtgctgtat aaatgtgccg gtctcctcct ggcacatctt tcaccataca aaaagcagcc
    33961 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    34021 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    34081 atttctgcaa cagttttatt gatcctgatt atggatccgc tcggaaacct acctattttc
    34141 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    34201 cttattgctc tccttgtgat gctggtgttc ctgtttgcgg gcgagaaaat tctggcattt
    34261 cttagcctgc gggcagaaac cgtctccatt tctggcggca tcattctgtt tctgatcgcc
    34321 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    34381 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    34441 atgttgttgt ctcaccagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    34501 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    34561 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    34621 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aaggttcaaa caggtgttgc
    34681 acacaaccag acgcggcacc tgttttatta cctgaactga taatgacctt cgcccaatcg
    34741 gataaccctt ccctcttctt ctgcgcggtt cagccacaca ctaatttgtt cgatggttaa
    34801 accagtacaa gacgccagcc tttcaatact tatcgattcc tttgcaagga tcgccagttc
    34861 cgccacaaag agttgataaa aatcgacagg ggtatgcgcg ggcattactg cgtaatttgc
    34921 gtcatcttcg tgcagcgaaa acagttctgg ttgagcgtta cggagtgctc tgtcatacat
    34981 cagcccagga acgtctggca gtctgatcgt cacattctca gcctgttcag cactccatga
    35041 cgccccattt tctactaatc gtgaattggc tgattgcata tcctgatttg gtttaacctg
    35101 catgggcagc cactgatgtt ttaatgcctc catcgccccg gttatcgttc caccggtcat
    35161 tcccgcacgt acaaccattg cgctttcagc aaggcaataa atatatttat tccgcgccat
    35221 cgcattgccg acggtgaaac gtacctctgg gtaaaacggc gaaatcaaca ccaggttgcc
    35281 tgctataagc ccttcacgcc atttcactaa cgtactcgtt tttaacaagc tatcagctaa
    35341 tacgccaacg gcagttcccc cgacctccag agccgaggcc attgcacatt catcaatacc
    35401 tcgcgcacca ccagagataa cgcaaatccc ctgttgggcc agtttcgcgg ccagttgttg
    35461 ggtatagcgc aaatcgtcag tcggagcatc tcgcgagcca acaatcgcca taccttccgc
    35521 ttgcagtaat gctttattcc cgcagccaaa taaaacggga ggtgcatccg ttcgcaatcg
    35581 gtttttcaag cgaacgggat aatcggcatc tccgcgggtt aagatccata aaccggcgcg
    35641 ttgccactta tctacagcca acgccagact atgaccacgc gccagcaagc caagaattcg
    35701 ctcctgagaa atacgcggat cgctccagcc tgtaagtttc tcttgcggtt gcggcaccag
    35761 aagctcggcg ggactgatac gttggtgctt cagccataat gcaaacttcc cccattcact
    35821 attactaaga ggtttatact cactcgccgc cgcgcgagaa aaatcgctgg ttagcaggag
    35881 agttgcttga gcattggctg aaagattcat caatttttta ccgaggtaga agcaagggca
    35941 agaggataaa ccgtcgggca acctgcctgg cgtaacagtg ctgtcagaac tgtcagcgtc
    36001 catgcagaat ccacgatatc atcaaccagc aacgccggac ctggcatcaa aggaggggta
    36061 atcacaaacg ccccgtcgag attttgacac tggtgaaaac ggttttgctg cattttctgc
    36121 ggtggattgt ccacgacttt ttcaatggca tcaataaaag gtaagccaag tttcgccgcc
    36181 agtcgccggg caaaatcagg aaccaggtcg aggtgcctta atgaaggaac gcagcatacc
    36241 caggtgggtt caggatgcgg attccagcgt tggcgaacca tttccgcaca tgcctccacc
    36301 agctcatcac tgaagcgccc cgtttttttc tcctgggcta cctgctttcc ccacccggaa
    36361 tctccccaac gagaaaggat tcgccccgta gatccttgta aacccgcagg caagttccct
    36421 ttaaatccgt attgcgtaaa agccccagat gccacctgtt tattgagatt taatggcagg
    36481 tcagcgtgtt taataaataa atttgccgca tggagtaagt cgctatcaat gtcgggcgac
    36541 aacagcggat gctgaagaca actgctacat ttaccacaac gttcggcact tggctcatcg
    36601 agtgcgcggc gcagaaattg cattttgcac tccttggtcg tgatatagct ttcaacgtct
    36661 gccaactcac tttttctctg attcatcaaa tggttaattc gttcatgatc aggagaaaaa
    36721 ctgacgatag ttctgcgcca taatttctcg gtatacacca caggcgatgg attttcagct
    36781 accaataatt tcagtgcttt ttctatttgc ccataacgaa gattcgtccg ttgttcaatg
    36841 cctcgtaggg taagaccgtc attctcgcta agtacgttga gtatttcatg aatttgcgcc
    36901 tccgcaggaa aggcgctttc acggaagaat tgatgaatag cgcggtcttc accaccacaa
    36961 agcaatatgc caactgcgga atctatagca cgcccggcac gccccacctg ttggtagtaa
    37021 ccgacaatag accccggcat ctgataatga atgacgaaac ctaaatcagg tttatcaaat
    37081 cccataccta atgccgtcgt cgcgaccagc actttgattt tatttgccag cagtgcctgc
    37141 tccagatact ccctggcagt attagaatct tccgcacctt cacaagtcac gccgctgtaa
    37201 taagcaaatg cactgatacc atttttcctt aaccactcgg cgacaagttc agcatcgcga
    37261 gtcgtcaggg tataaacaat tcccgatttg gaaaactgag ggataaccgt tgctaaccat
    37321 gccagacggg atgactgttc tcctaagact aaggcatcaa gcgccaaact ttcgcgagcc
    37381 agcgttccac gctgaatcac aatgtcaccc aattgctgac ggatatcctc aacgacacgg
    37441 ttattcgctg tcgcggttgt acccagaata ggggtattcg caggcagttg gcgcaaaata
    37501 tctaatatac gtcggtaatc cgggcggaaa tcgtggcccc aatcagagat gcagtgcgcc
    37561 tcatcgacca ccagtaaacc gatgcggtca gcgactggat ataaaaccgt ttcaagaaaa
    37621 tcctgattcg ccaaccgttc aggtgaaatc agcagacaat ctacgccacc ttgcagcaac
    37681 ttatcgctaa tgcgctgcca ttcttccctg tttgtagaat tcaacgtttc agccgtaata
    37741 cctaaccgtt cagcggcggc aacctggtta cgcatcaggg caagcaaagg agagatgata
    37801 attgtcgggc cagcgccgcg atcgcggaag attttactgg cgatgaagta cacggcactt
    37861 ttcccccagc cggtgcgctg tacaaccagt agttttcgcc gctgattaac caccgcatct
    37921 atagcttccc attgcccttc cctgaaagta gcgctggtgt tacttaacat ccgctgtaac
    37981 agcaattcag ctccgtgttt ctccatcatc ttctccctcg tccgttaaca cagtgattag
    38041 aacggataat gttccggtct ttttgcgaga gagatcaaaa agatgaagac aggagaaaag
    38101 agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa ctcaacagct
    38161 ggaacgcaat catcccaacg atggcaccga cagtgccgag gatggtttcc atcatggtcc
    38221 aggttttaag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaacccg
    38281 cgtcgttaac gtggctgaca acaatcgaac caccggcgat acaaatcgac agtgccgcca
    38341 tttgcgcacc ggagtagttc agttgttcaa tgaccggcat taccaatccc accgccgtta
    38401 aacaggcaac ggtagcagaa ccctgaatga tgcgcactgc agctgccagc acgaagcagg
    38461 tgatggcaat cggcaggccc atgccggtta acgcttcgcc cagtgccgga ccgacaccag
    38521 agtcaaccag cacctgtttg aacacgccgc ccgcaccaat caccagcaga atgatccccg
    38581 ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcata ccctgacgca
    38641 ttgccagacc gtaaatcgcc accaggcaag caaccagaat cgcggtaaac ggatgaccaa
    38701 taaactcgaa ccattcgtaa gcggtagaac cttctggcac aaaacgcgcg gcaatggttt
    38761 tcagccccac cagcaccagt ggcaacagga tcagcgacag gctgaatccg aaggacggca
    38821 ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc
    38881 ggctgatgaa attaccccac agcggcccgg caataatcat tcccggaatt gccgcgcaca
    38941 ggccaatcag gatcatccag ccaaagtcgg cgttcatctg cgatgccagc agcattggcg
    39001 ctggcccagg caccaggaac gcagccgctg ccgccacgcc ggcgaataat gggattacca
    39061 gcttcaccag gttcgtgccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa
    39121 caatcgccac ttcaaagaac agcggtagcg cacaaaccag ccccgcaagg ccgatggcat
    39181 aatgcacgcg gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgaccgcgc
    39241 cggtttcatg taagatctta ccaaacatgg ctcccagggc gacaaccacc gccaggaagc
    39301 cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg
    39361 aaaaaaggcc agcccccatg gacaccacca ttaaagccag gaaagcgtgc atacgcgcct
    39421 tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg
    39481 tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca
    39541 gcggttgatc aatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg
    39601 tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc
    39661 ggctttcaat cacatcaaaa tcgcctttca ggtagatgaa agagagatta ggattacctt
    39721 cacgcagcaa gtcgcgatag tgttttttca gtgcagaaca gacgatcagc gatactttat
    39781 tggtgcgctg catagcaaac gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt
    39841 cgtcattcag cggttcgcca gacgccattt tttcgatatt gcagcgcgga tggaggaaat
    39901 cgccatcaag aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc
    39961 cgctgcccga tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag
    40021 tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt
    40081 ccagccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta
    40141 cttcaaattt gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca
    40201 ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgctcagcg ccaatgctgc
    40261 ccatccgctc acgcggcgtc agcacactcg ccagccgtgg ctccatcacc tgaccaatgt
    40321 catgaccgtg gaaaccggca atcgccatat cgtcaggaac tttcaacccc agacgctgac
    40381 attcaaacgc cgcgccgacc gccaggtcat cattagtaca gaacacgcca tccagctgcg
    40441 gatattcccg ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct
    40501 caaccatcac gctatacggc accaggcctg catccagcat cgcctgttcg tatcccttct
    40561 gtttgatgat agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc
    40621 gagcaataat ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga
    40681 tatcgagaca cggcgactta ctgtccatca actccaccac cggaatcccc gccacttcaa
    40741 tcatctttaa ggtgcgtggc gtgtgggtac gttcagtgag gatcaggccg tcgatattcc
    40801 atgagagcat tgattcgagg cgttcttgct ccatttccgg tttataaccg tagtgcgcca
    40861 gcatggtttg ataaccgtgc gcgtcggtga cactttcgat tccgcgtaat acttccgcga
    40921 aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga
    40981 gtatatcggg cgcgcgattg ggaatataac ccagttcatc aagagcggcg gcaatcttgc
    41041 cgcgtagagc gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg
    41101 tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga
    41161 aggtacaaaa gagatagatt ctcttagttt aacacggacg catcacaacc ttcctgattc
    41221 ctcaggaagg tttgtgagat aaatttagac tggcggtaaa tcgaacagca ggatttcact
    41281 gtcgctatca gcatggatgg aaatcgcctg ctcatcccag attgccagac catcgctggt
    41341 cgaagctttt acgccgttaa tggtgacatt gcctttcacc acctggatcc agatgcggcg
    41401 ttcagcggca atctgatgca ccgactgctc atctttcacc agcgcccaac gatacagttc
    41461 catatcctga tgcactttca atgaaccatc tcgcgcatcc ggcgaaagca ccagttgttt
    41521 gccctgcacg gcatcgaagc gacgctgttc ataacgcggc gtaataccgt tttcttcagg
    41581 catgatccag atctgataca gatgcagacg ctcggtgctg cttgggttgt actctgagtg
    41641 acgaataccc gtacccgcac tcataatctg gaactcgccc gccggaacct gctctttatt
    41701 gcccatgctg tcctggtgct caacagtacc ttccagcacg taggtcaaaa tttccatatc
    41761 tttatgcggg tgagtgccga agccctgccc tgcttcaata acgtcgtcgt taatcacgcg
    41821 cagcgcagag aagcccataa agttcggatc gtaatagttg gcaaaagaga aagtatgcca
    41881 ggagtccagc cagccatgat ttgcatgacc gcgttcattt gctttgcgta agtagatcat
    41941 ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg gacccgatcc gcctgggatg
    42001 atagagggtg aaaattgacc cctctgttca aaaaaattga actattcgag gggccatcaa
    42061 acttacctcg cgagactcac tgcggaggga gaagcttgct caaaaccgcg ttcaaggatt
    42121 tccagattcg taagaacttc agattccttg acgtaatttg gcgcaccgtt ggtaatggtt
    42181 tgatacaacg catcataaac gcgcccataa tcgcccatct ctggcttcat ctcttctctt
    42241 accgtcacgc cctcgtcatt gacatactcc agcacaccga ccgaatcatc cgctgcgaat
    42301 cctggttcgc ccggcataat attagccttc aggctggttt cctgctggtc gataccgtat
    42361 ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga
    42421 tggctggttt tgacgatggc ttttagatcg ccataaaaca gctgcgcttc aaaggtgtcg
    42481 tccggattgg ctttattacg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg
    42541 aacagagaaa taatctggtc catcgtatgc acaccaaggc catagaacgc gccatcctgc
    42601 ggcagccccg gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttcg
    42661 acaatctctc ccagcttgcc gctttcaatc gcttttttcg ctgtcaggaa gcaggaatca
    42721 aagcgacgat tctgatacgg cgtaacgatc agccctttgc ttttcgccag tgcaaacagc
    42781 tctttcgcct gcgcaattgt cggagtgaac ggtttttcga ccagcacatt tttcccggct
    42841 tccagcgcgc gcttcgcgta ctcgaagtgg ctgtccgcgt gggtgcagac gacaaccagc
    42901 ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg tgaaatggat atgggaataa
    42961 atgggggcct gttcttccgg cttcgcatgg cggcgaaaaa tatgcgcgac atgccagcta
    43021 tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata
    43081 aaggcgcagt taatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc
    43141 aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcacttc ccgaggataa
    43201 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    43261 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    43321 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    43381 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    43441 agatagtaat acgccacgca gaaacacgcg attacgaggc catcaggcag attcacgccc
    43501 agccggaggt gtattacaac acgctacagg tgcctcatcc ttcagaacaa atgtggctgg
    43561 aacgactcac cgctcgccct ggcatcaagc agttagtcgc ctgtattgat ggaatcgtcg
    43621 taggccatct caccattgac gtgcaacaac gcccacgccg cagtcatgtt gccgattttg
    43681 gtatctgtgt cgactcccgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    43741 tgattgaaat gtgcgacaac tggttgcggg tagatcgtat cgagctaacg gtgttcgtcg
    43801 ataacgcgcc agcaattaag gtctataaaa aatttggctt tgagattgaa gggactggca
    43861 agaagtacgc attacgaaac ggtgaatatg tcgatgcgta ttatatggcg cgtgtgaaat
    43921 agagaatttg caatctacgt ttgtcattta agccctcacc taccgggaga gggcttaaga
    43981 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcacc gtacagctca
    44041 ccgtccggcc caaccataat gctttgtgta ctgcccatcg cctctttcag cgccactttc
    44101 tgaccttttg cttccagcag cttgatcgta tccgggctaa accctttttc gacacgcagc
    44161 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatt
    44221 ccataatcga tgctattcac caccatttgc agcacagtgg tgataatccg gctaccgcct
    44281 gggcttccgg tgaccagcca ggttttacca tccttgacca caatggttgg tgacatcgac
    44341 gacagcgggc gtttattcgg cccgacggcg ttggcatcgc cgcccaccag cccgtaaacg
    44401 ttcggtacgc ttggtttggc ggagaaatca tccatctggt tattgagcaa aataccgctc
    44461 tcgcccgcga caatgcccgt accgaaggtg gtgttcagtg tataggtcac cgccacggcg
    44521 ttaccgtctt tatccactac tgagtaatgg gtagtttgat tactctcata aggcgcaagc
    44581 ttgccaggac gaatttcgct ggatggcttc gctttattga tatcgatttg atcggcaata
    44641 gatttggcat aggctttatt ggtcagcgcc tgccacggta ctttgacaaa atccgggtcg
    44701 ccaagatatt ccgagcggtc ggcgtaggcg tatttctcag cttctgccat gatttgcatc
    44761 gcgtcggcgc tgccaaagcc gtacttctgc atatcgaagt tttccagaat attgaggatt
    44821 tgtacgatat gaataccgcc ggaagatggc ggtggcatgg agtaaacctg atacccgcga
    44881 taatcgccgc ttatcggtgt gcgttcgacc gctttatatg ccgctaaatc ttctttgctg
    44941 atcaagccac cgtttttctg catctcctgg gcaatctgtt ccgctatcgt gcctttgtag
    45001 aattcatccg ggccgttttc ggcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    45061 agcttgtcgc cctttttcag cggttcgccc tctttccaga agatagcttt gctgttttcg
    45121 tggttcggca gcacttcact gccgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    45181 ataaaaccat cgcgtgccag tttaaacgcc ggttgcacca ctttattcaa cggcatggtg
    45241 ccgtatttat ccagcgccaa cgagaaaccc gctaccgtac cgggtgtgcc ggaagccaga
    45301 tgcgaagtga gtgatttttt gctgtccgtg ttgccctgat catcgaggaa catatcgcgg
    45361 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    45421 cggatcagca taaaaccacc gccgcccaga ttccctgcct gaggatgcgt taccgccagc
    45481 gcgtagccca ccgccacggc agcatcaacg gcattcccgc cctccttgag gatatccact
    45541 cccacctgag tggcagtaac atctacagac gccaccatcc cttgtttcgc gcgtaccgga
    45601 tggaagacat cttcctccac accataagaa acaggaggcg cggctaccgt actaaaacaa
    45661 cttcctgtga gcagagcagc aatggccacc cggcgtaaaa acgtcggttt tatcatcgtt
    45721 attctccaga gattaagggg caaccccagc taagcctggt atataactct gaattaatca
    45781 tcgttttgcc gggaagcgag taaacttaaa gaatatcttc agaggaggat acgcgatgaa
    45841 acgacttctg cttttgacgg tactcctgcc gtttgtcggc tttgcacagc ccattaatac
    45901 tctgaacaac cctaaccagc cgggatatca gatccccagc cagcagcgga tgcaaactca
    45961 aatgcagacg cagcaaattc agcaaaaagg gatgctgaac cagcaactga aaacgcaaac
    46021 gcagctgcaa caacagcatt tagaaaacca gataaacaat aattctcagc gggtgttgca
    46081 gtcacaaccg ggggagcgaa atcccgcccg gcagcaaatg ctgcccaaca ccaacggcgg
    46141 gatgttaaac agcaaccgta atccggatag ttcgttgaat cagcagcata tgctgccgga
    46201 gaggagaaac ggcgacatgc tgaatcagcc cagcacgccg cagcctgaca ttccgttgaa
    46261 aactattggg ccgtaaagtt cggaccaatc acgtcaatcg catcggtaca gatgcaatcc
    46321 acgccccagc gcagcaactc tgccgcgcgc tggggtttat tgacggtata aaccagaatt
    46381 cgcagtcctg catctttcaa ctgcatcact cgcgctttat cgagcaactt ataattgaga
    46441 tgaatagaga cgcagcccag ccgcgcggtc aattctcgcc agtcgtcgcg ccattcatca
    46501 agcaataaac cacgcggcag ttccggtgct gcctgttgag cggcttctaa agcatcaatc
    46561 tcaaacgatg acagcagcgg cggcgtcata ccggtccaca gttcgcgtgc cgccaacgcc
    46621 accattttgc ccgttaatgg tccagtgtca gtggtgggtt tgatttcgat attcgccatc
    46681 atcccgtgtt tgcgacagcg ttccgccacc tgcgaaagca acggcagtgg ctcaccttta
    46741 aacgctttgc tgtaccaact gcctgcatcc acgcgcagta aatcctgcca gttcagttca
    46801 cccgcgacgc cccagccgtt gctggtgcgt tcaagattgt cgtcatggag caggaagatc
    46861 tcgccatctt tcgataactt cgcgtcaaat tcgatcatct tatgaccgta ttttgccccg
    46921 acgtcgattg ctgccagggt gttctccggg gccagtttac cgccgccacg atgggcgacg
    46981 atgcggggat aaggccagtt actcatactc gttgtcctgt ttcaccatca aaaagatgta
    47041 gctgattttc cggcagatgc agccacagcg tgctgcctgc cgtcgggcgc tcctgatgtg
    47101 ccagtcgcac caccagcttc tgttcgcccc aatgtccgtg cgccaggtta tctgcgccga
    47161 ggatctccag cgtgtccatc accagcggca caccaccttc tgcctgcgag cttagcgcta
    47221 tatgttccgg gcgaatgccg agggtcattt tacgcccgac atactgacgg tagccaccgt
    47281 ttagcggcag cgcaataccg ccatccagtt cgaaatgcgt accttcgtta ttcacacggc
    47341 cagccagtag gttcatcgcc gggctgccga taaaactcgc cacaaacagg ctggcgggct
    47401 tttcgtacac ttcaactggc gtgccaatct gttcggcaac gccgccgttc atcaccatta
    47461 ctcgctgggc gagcgtcatc gcctcaacct gatcgtgggt aacgtagagt gaagtcgttt
    47521 tcaggcgacg gtgcagctgt tgcagttcaa gacgcatctg cacacgcaac ttggcatcga
    47581 ggttagagag cggctcatca aataggaaca ctgccggctc acgcacaatc gcacgcccca
    47641 tcgccacacg ctgacgctga ccgccagata gctcacgcgg gcggcgctta agcaggccgt
    47701 ccagctccag aatgcgcgcc gcttctttaa cacgttcggc aatttgttgc ttgcccatgc
    47761 cgcgaatttt cagcccccac gccatgttct cctctacgct catatgcgga tagagcgcgt
    47821 agttctggaa caccatcgca atcccgcgat ctttcggctc catttcggtg acacgcttgc
    47881 ggtcgatcca gatatcgccc gtcgttaccc gctccagtcc ggccaccatt cgcaataacg
    47941 tcgatttccc gcagccagac ggcccgacca tcacgataaa ttcgccatcc gccacatcaa
    48001 gggtcagcgg tttaatgacc tgggttttgc catcccagct tttggttact gcctgtaatt
    48061 tcagtcctgc catcttattt ctcactatcg accaggccgc gcacgaaggc acgctgcatc
    48121 actaaaacaa tcactactgg tgggatcagg gttaacaaca tcgccgccat caccgagttc
    48181 cattccgttg tgccttcgcc tgtggcgatc atccctttaa tccctgccac ggtggtgccg
    48241 agatcaacgt cggtaataat caacaacggc cacaaatact ggttccagcc gtaaataaag
    48301 gtgatcacaa acagcgccgc cagattggtt ttggacagcg gaaaaacgat gtcgcaaaag
    48361 aaacgcattg gcgacgcgcc gtcgatccgc gcggcttcca ccagttcatc cggtagggtc
    48421 ataaagaact ggcgaaacag aaacgtggcg gtcgccgagg ccatcagcgg cagcgttaaa
    48481 cctgcgtagc tgtcgagcat tttcaggttg gcgatgactt ccaccgtcgg gaagatacgc
    48541 acttcaaccg gcagcatcag ggtgataaaa atcatccaga agaagaggtt acgtagcgga
    48601 aaacgaaacc agacaatggc aaatgccgag agcatcgaga cggtaatttt gccgagcgta
    48661 atgctgaacg ccatcacaaa gctgttaagc aacatccgcc agaacggcgc gctattcgtg
    48721 cctaccccgt ttacccagat gttgtggatg ttttccagca gatgtgtgcc gggaataagc
    48781 gtcatcggcg cggcgtagac ttcttgttta tccagcgtcg ccgcgacaaa cgccacgtac
    48841 agcgggaaga ggatcaccgc gatcccgagg atcagcatgg tatggctgaa tattgtcagc
    48901 cacggacggt tctcaatcat tggtaacgta ccttgctttc aacatagcgg aactgcacca
    48961 ccgtcagcac gatgacgagg aacatcaaca ccaccgactg cgcggcagac gaagccaaat
    49021 ccagtccggt aaagccttca cggtagatct tataaatcag cgtcgtggtg gcctgtactg
    49081 gcccgccgga cgtggcggcg tcgatcaccg ggaaggtgtc gaagaaggca tacaccaggt
    49141 tcaccaccag caggaagaaa ctcaccggag cgataagcgg cagcgcaatc ttaaagaagc
    49201 ggcgaatcgg gccagcccca tcaatggctg cggcttcgat caacgaacgg gggatggatt
    49261 gcagcgcggc atagaagaac agaaagttgt agctgatttg cttccacact gaggcaaaca
    49321 ccaccaggaa catcgcctga ccgctgtttt gcgcgtggtt ccagtcgtag ccgaactcag
    49381 cgagaaaatg ggtgatcagc ccgcgaccgg ggttaaacag aaagatccac aatacggcgg
    49441 caacggcggg agccacggca tacggtagca acatcaaggt ttgatagaaa cgactgccgc
    49501 gcacgatgta ctccaccagc gctgcgaaga acagcgatac cagcaaaccg ctgacggtga
    49561 caaaggtgct gaattttatc gtcgtccaga aggcgtcgag atagtagctg tcgtgaaaca
    49621 gcgtgacgaa attatccagg ccgacaaact ggctggagaa accaaacgga tcgacgcttt
    49681 gtagcgagta ccacaacgct tcgcccgcag gccagataaa aaagataacg gtgatgatga
    49741 gctgcggcgc gaccagcaaa taaggtagcc agcgcgagcg gaacaccgga cgggatgatg
    49801 gcattactgt taattcctga actgtgccgg atgcgcttcg cttatccggc ctacacgcga
    49861 tgccaggtca gataagcgaa acgcatccga cattacactg attaagactt cgtcgatttc
    49921 tcaaagcggc gcagtaactg gttcccacgc tcaacggcag tatccagcgc ctgctgtggt
    49981 gtcttcttac cggtccacac gctctccagc tcttcatcca caatcacgcg gatctgcggc
    50041 atgttgccca gacgcaaacc tttggtgaac ggcaacggtg gcttgttcag catctgacgc
    50101 gtggcaatat ccgctcctgg gtttttctcg taaaagccct gctcacgggt caggtcatac
    50161 gccgctttag tgattggtag ataaccggtt ttctgatgcc actcggcagc gttttctggt
    50221 tttgcgagga agtcgaggaa cttcgccacg ccggtgtaag tttctttatc tttaccctgc
    50281 atcacccaca ggctggcccc gccgataatg gcgttttgcg gcgcgtcttt cgcatcggcg
    50341 tcgtaaggca tcatgcctac gccatagtta aatttggcgt actcgcgaat gttggcaaga
    50401 gaaccggaag aggcggtcgt catcgcgcaa tcaccgttat agaacttctc ggtggattca
    50461 tccttacgcc caacgtagct gaaatcgccc ttcttgttca tctcttcgag catggcgatg
    50521 tgtttcacct gctccggctt gttgaactca agcaccgcgt ctgtgccgtc aaagccgttg
    50581 tttttgctgg caaacggcag accgttccag gcgctaaagt tttccagttg gatccagccc
    50641 tgccagccgc tggcgtagcc gcacttcatg ccggaggctt ttagttttgc ggcatagtcc
    50701 gccagatcct gccaggtttt tggcggctgt tccgggtcta atcctgcttt cttgaaggcg
    50761 tctttgttgt aatagagaac gggggtcgaa ctgttgaacg gctgggaaag taagtgccca
    50821 gttttgctgt cggagtagta accggaaacc gtcggcacaa actgcgactc atcgaattga
    50881 atccttgcct ctttaaatac gtcatacacc ggtttaatgg ctttcgacgc catcatggtg
    50941 gcggtgccaa cttcataaac ctgcaaaata gccggagcgt tgccggtacg aaatgcggca
    51001 atccccgcgc ttaaattctg ttcgtaattg cctttatagg tcggtacaat tttgtaatcc
    51061 gggttttcgg cgttaaaacg ttgggccaga gaatccacct ctttacccag ttccccttcc
    51121 atagaatgcc agaacggaat ggtcgtcact gcctgcgcat ttgccattaa agccagtccg
    51181 agcgccagtg ctgaagctgt ataacgtaac ggtttcattg attatctctc ttgttgtacc
    51241 gaatgcgcga attcacgcgt tttatgctcg cggagtaaca tgacatgctc gaattacaga
    51301 aaaataacta ttttgttaca tttttaagat agtaaggtgt cagaaagatg acaaggcggt
    51361 gacggcgcgg gcgagggaaa atgggagatg gggcacggat aagcggaaaa atagaaggtc
    51421 tgaatcaaac tctacagatt actcatcgtt tcatgccgga tgcggcgtaa acgccttatc
    51481 cggcctacaa gatcgtgcaa attcaatata ttgcaactca cccagtaggc ctgataagcg
    51541 cagcgcatca ggcaatttta catttgtcac ctgtctcaaa ggagtctttt gactccttat
    51601 caatcaacgt gttattaccc gcctaaatat gcacttctca ctgcttcatt cgccagcagc
    51661 gcgtcgccgg tatcggagag cactacatga ccgttttcca gcacgtagcc gcgatcagcc
    51721 agctttagcg cctggttggc gttctgctcg acgaggaaga tggtcatccc ctgctcgcgc
    51781 agttgttcga tggtgtcgaa aatttgctgg atgataatcg gtgccagccc gagcgatggc
    51841 tcatcaagca gtagcaaacg cgggttactc atcaaagcgc gaccaatcgc cagcatctgc
    51901 tgttcaccgc cagacatagt gcccgctcgc tgaacacggc gctcatgcag acgcggaaac
    51961 agttcataga cccactttat gcgctcctgg aactggtcgc gttcggcaaa aaagccgccc
    52021 atcgccaggt tctcttccac cgtcatccgc gagaagacgc gacgcccttc cgggacaatc
    52081 gctaccgctt cgcgcatgat tttcgccgtc tgccagtcgg taatgtcttt atcatcaaac
    52141 acaattcgcc cgctggtggc gcgcggatcg ccgcataacg tgccgagcaa ggtggttttc
    52201 cccgccccgt tcgcgccaat gagggtgaca atctcgccct gattgatatg caggctcacc
    52261 tcatgcagcg cctggatttt gccgtagtgg gcgctgactt tgtcaaagga caacatgact
    52321 ttttccatct tatgcctcac ctaaataggc acggatcaca tctgggttat tacggatctg
    52381 ctccggtgta ccgtttgcca gcggcgtccc ctgattgacc acgtaaattc tgtctgaaat
    52441 ccccatcacc agcttcatat cgtgttcaat caacaagata gtggtgttgt gatggttacg
    52501 cagttcggca atcagctcat ccagctcttt cgtctctttc gggttaagac ctgctgccgg
    52561 ttcgtcgagc attaaaatct ccggctgcgt caccatacag cgggcaatct ccagacggcg
    52621 ctggtcacca taggccaggt tactcgcctg acggttggca tgttccagca aaccaatgcg
    52681 ctcaagccag gtggcggcgc ggtcgagcgc ttcgctctgg gcgcgacgga aagagggggt
    52741 tttcaacagg ccggagaaca gcccagtttt cagttgctga tgctgtgcca ccagcaggtt
    52801 ttcaatcacc gtcatttcac ggaacagacg tacatgctgg aaggtgcgca ccacgcccat
    52861 gcgggcaatt tgctgccccg gtaaaccttc caggtgctga tcgcgcagta aaatggtgcc
    52921 gccggtgggt ttatagaatc cggtcaggca gttgaagacc gtggtttttc cggcaccgtt
    52981 agggccgatt aacgagacga tctcctgcgg gtacagttca agattgacgt tgttcaccgc
    53041 cagcaggccg ccgaagcgca tcatcaggcc gttaacagat aataatggct gactcatgcc
    53101 tgctctcctt ttgctgcgcc gtttttcagc ttgagttgcg ggcgcgtcat gggcagcaag
    53161 ccctgcggac gccagatcat catcagcacc atcaaaccac cgagcattaa catgctgtat
    53221 tcgttgaaat cacgcatcaa ctcgcgcgat accaccagca aaacagccgc aagaatcact
    53281 gcaaattgcg agcccatacc gccgagcacc actatcgcca gcacaaacgc cgattcggca
    53341 aaagtgaagg attcagggct gacaaagccc tggcgcgcag caaacaacgt tccggcaaaa
    53401 ccggcaaacg cagcgctgat ggtaaaggca gtcagcttga tacgacgcgg gcttaagccc
    53461 agcgaacgac aagcgatttc atcttcacgc aacgcttccc acgcacgccc cagcggcatc
    53521 cgcagcaggc ggttaatgac aaacaggctt aacaccacta gcagcaacgc caccaggtag
    53581 aggaagatga cgcgatcgga gggatcgtat ttcaggccaa agaaattgct gaacgtgtcc
    53641 cagccgcctt cgcgggcggt acggctgaac tcgagtccga agagcgtcgg tttcggaatc
    53701 tgactgatac cgttcggtcc gccggtaatt tcggtgttat tgagcagcaa tatgcgcaca
    53761 atttcgccga acccgagggt aacgatcgcc agatagtcac cgcgcagacg cagcaccgga
    53821 aaaccgagca ggaagcccgc cgccgccgcc attaacccgg caatcggcag acaggtccag
    53881 aagcccaggc cgtaatagtg attgagcagc gcaaaagtgt aagcgccgat ggcgtaaaag
    53941 ccgccgtatc ccagcaccag cagaccagaa agaccaacaa ccacgttcag cccgagaccg
    54001 agaataatgt agatcatggt cagagtggcg atatccaccg tcccacgcga caccataaac
    54061 ggccacgcca ctgcaagcac cagcagcgcc acgaggaaca gtttctgctt caccgtggag
    54121 ccatcaatcg cgggcagaat aaacttcggc ccggaaacgc tttttagccc tttctggaaa
    54181 gcaggccgca caagctggaa gaaaaagacc accgccgtgc cgataaacac ccactgccaa
    54241 cggatctctg aagccgtgtc gaccaccagt ttggtgccat ccagctccag ttgcacgccc
    54301 ataaagacgc ccgccagcac aaagaacata gcggcagaga gcagcgccat tgcaatatgc
    54361 atcggtttca tactttctct acctccgggc gacccagaat accggtcggc atcaccagca
    54421 gcaccaggat cagcagggcg aatgagacca catctttata ttccgtactc agataggcag
    54481 aagagagcgc ctccgcaatc cccaggatca gcccgccaat catcgccccc ggaatgctgc
    54541 caatcccacc gagcaccgca gcggtaaagg ctttcatccc ggccataaag ccgatgtagg
    54601 ggttaattac gccgtagaac tgaccgagca gcacgcccgc cactgccgcc atcgccgcgc
    54661 caatcacaaa ggtcagcgca atcacccggt cggtgttgat gccaagcagg ctcgccattt
    54721 tcagatcttc cgcgcaggca cggcaggcgc gccccatacg ggaatagcga atgaaaatcg
    54781 tcagcgccag catggcgagg aaagtaacaa tccatatcac cgcctgcatg gtggtaatag
    54841 acgcagagaa gttttcgcta tggcccacca cccactgacc gttaaacagg ctgggcagcg
    54901 ccacatcgcg cgaaccttcg gtcaggctga cgtagttttg caggaagata gacatgccga
    54961 ttgccgagat gagtgcaatc aggcgcttag agttgcgcac cgggcgatag gctacccgtt
    55021 cgatactcca gccgtaggcg ctggcaatga cgattgcgcc gacgaatccc gccgccacca
    55081 gcagccagcc ggtatcaatg cccagcatca tcagtgcggc gatgatcata aaggagacgt
    55141 agctgccaat catgtaaacc tcgccgtggg cgaagttgat catgccgata atgccgtaaa
    55201 ccatggtgta gccgatggct atcagcgcgt aggtactgcc cagcgtgacg ccgttaaaca
    55261 tctgctgcaa gaaatacaaa aactgctcag acataaggta acctttctaa acccgcccgt
    55321 ttttcacgga cggtgggatg atcacttggc tgccgtggat gaaccgtcgg cgtgccactg
    55381 gaagacacca aaatcgaatc ccttaagatc gcctttttca tcccagttca gcggcccaat
    55441 tacggtgttt gcaccgttag cttttaaatc tttcaccagc gccagcggct catcgctgcc
    55501 ggtacgttca agggcagttg ccagagattg caccgccgcg taggtgatcc agacataggg
    55561 cccggacgga tctttcttgt cggctttcag cgcatcaacg atgccctggt ttgtcggatc
    55621 ctggtcatag cgttttggca tggtgaccaa catgccttca gcggcatcac cggcaatgtt
    55681 cgacagtgac gcattaccga caccttccgg ccccataaac tgggttttca ggccaacgga
    55741 acgggcctgg cgcagcatct gccccatttc cgggtagtaa ccgccgtagt aaacgaagtc
    55801 gatgttttct tttttcaggc gggcgatcag cgcggagaaa tctttctctc cagcggtaat
    55861 gccgtcgaag aagacgacgt tggcgttagc cgctttcagc ccgtcctgca ccgaacgtgc
    55921 cagcccttcg ccatactgtt gtttgtcgtg aatgatggcg atgcgctggg gcttcaccgt
    55981 ctcaagaatg tattttgccg ccgttggccc ctgggaagag tccagcccgg cagtacgcat
    56041 gatgtattga taaccgcgtt gggtcagctc cggattggtc gctcccggcg agatcatcag
    56101 aataccttcg tcttcataga tatctgatgc aggttgggta gaggaggaac acaagtgccc
    56161 aataacgtat ttaatgccgt cgttaacgat tttgttggcg accgcaacgg cttgtttcgg
    56221 gtcgcaggcg tcgtcatatt ccacgccaac cagtttatcg cccttaattc cccctttggc
    56281 attaatgtct ttaattgcct gacgcgcgcc gttaaattcc atatcgcccc actgggcaat
    56341 cgggccggac atcgcgccga caacggcgac tttaatatcg tcagccatag cggtgtgtga
    56401 aattgccagt gcaatcatcc ctgcgatgat agttttcgca ttccgtttca tagtcaaaaa
    56461 tccccattcg tgatgttgtg ttgctttgtt cttatgtgtt aacaaatcag actgttcttt
    56521 tttatactgc actgttttta cctgtctgat taagggttta gcgcagtatt ttgtgatgat
    56581 agcgattaaa atccctattt ttcagtcgat taagaacaga taatattctg aatttattga
    56641 tagataaaca gaaaaaagcg ccgttgtcag cataaaataa cggtacaaag ggcagaataa
    56701 ttcactacca tttaggggat tatgctggat atttttcatt ctctaatgtt ttaactttgt
    56761 aattattgct gaaaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg
    56821 attaaattat ttcgctacac tggttccact tttgtgattt acacgggttc cccatgaagc
    56881 tgaccatcat tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct
    56941 ggccggagta ttccccttcc tctttacagg ttgacgataa ccaccgcatc tacgccgcgc
    57001 gttttaacga gcgcctgctc gctgccgtgc gggtaacctt aagcggcacc gagggggcac
    57061 tggattctct acgcgtgcgg gaagtaaccc gacgtcgcgg tgtggggcaa tatctgctgg
    57121 aagaggtttt gcgtaacaat cctggcgttt catgttggtg gatggcggat gctggcgtgg
    57181 aagatcgcgg cgtgatgacg gcgtttatgc gagcactggg atttacggca caacagggtg
    57241 gctgggagaa gcgttaatcg tctggtgtaa aaagtagctc tatagatttg gtcgggaaaa
    57301 ataacgcatt ttcgttttct ccgaccaatg ggataaagcc cagcgactga taaaacgtat
    57361 gggctttttc attcagcgcc tcgacaaaaa gaccgtgaat acctaccgct aaagaggctg
    57421 accagacaac tttcatggca tgagcaacca gtgttgctcc ccatccctgc ccctgtaatg
    57481 aacgatcaat tgccagacgc ccaagagtta cgctgggaat atttttgtag ggaatttttt
    57541 tctgtttcga tttcgagggc agtgcggctc gttcaaaaca actgccggat aatgtgtaat
    57601 atcccagcac ctgacgttct ggagtgttgc gacacaggat atacgctcgc agaattttat
    57661 tgcgatgttg acgaaccaga tgtgtcgtca gaaagagatt taatgcttcc tcgccgcagt
    57721 cgaatcgctg tagatcataa tctgcatcat cggtcagaat ctctatcgtc agatcatcca
    57781 taaagtatta cattccctga agacgttttg ccgcacgttt tagcttttca cctggtgacg
    57841 gtggattact cagcgcatcc atcacccgcg tccaggattc ctcagtgaga atcatccgcc
    57901 gatgctgttc aatcacttcc gcagctcgct gcgaagcgct gttcaacata aattggctga
    57961 cggactgatt agatatcgct gccgcttcct caatcatact tttgtcatcg tcagttaaac
    58021 gcagatcaat acgctgcttt ttaacagcag acatgtgaac tctcctgctc cgtataatat
    58081 gtacggcata tcaccgtaca taagcatatc ctgaattgtt caatctttct acaaccattt
    58141 cgataaatat aaaaaaaccc gatggcagtt cagcgcatcg ggtattttca tttatctatc
    58201 tgttacttcg catcagtcgc cgtgccgttg gcgtgccagt caaacacacc gaactcaaag
    58261 cctttcagat cgcctttctc atcccagcta agcggcccca tcaccgtatc aaccgagttc
    58321 gctttcaggt atttagcgat ctccgccgga tcgtcagact gattcagccc cgcttgcaaa
    58381 gattgcagcg cggcgtaggt ggtccagacg aacgcgccgc ttgggtcctg ttttttcgct
    58441 ttgatcgcgt caacaatcgg tttgttcgcc ggaacctgat cgtagttctt cggcttggtc
    58501 accagcagcc cttccgctga ttcgcccgca atgttagaca gcgagacgtt agccacgcct
    58561 tctggcccca taaactgagt tttcagccct gccgcgcgtg cctgacgcag gatttgcccc
    58621 atttccgggt gataaccgcc gtagtaaacg aaatcgatat tctctttctt caaacgcgcc
    58681 accagggtag agaaatcttt ttccccggcg gtgatgccat caaagaacac cacgttagca
    58741 ttgcctttct tcaggccgtc ctgcactgca cgcgccagac cttcgccgta ctgctgtttg
    58801 tcgtgaacga tagcaatgcg ttgcggtttc actttctcaa gaatatattt cgccgccgtc
    58861 ggcccctggt cggagtccag gccggtggtg cgcaggatca gctgatagcc acgggcggtc
    58921 agctctggtg cggttgccgc tggggtgatc attaaaatgc cttcgtcttc gtagatgtca
    58981 gacgctggct gcgttgatga agagcagagg tgaccaatca cgtatttaat gccgtcgtta
    59041 acgactttgt tcgccaccgc aaccgcctgt ttcgggtcac aggcatcgtc atattttacg
    59101 atttgcagtt tgttgccttt gataccccct ttagcattga tatccgcaac cgcctgctct
    59161 gcgccagtaa actcctggtc accgtactgc gccaccggac cggacattgc gcctacgacg
    59221 gcgactttaa tatcttctgc cagagccata ttgctgaatg ccagcgcgat acatcctgcc
    59281 agtaacgctt taccctttat gttcatcctg agaatcccca ctcttctggt tattacgtgt
    59341 gttgtgatgt tgttgtgcag cactttattt cgttttatac gtagctaccc gtgctttagc
    59401 agcatactct gctaaaacat accccatttt tatgatattg gaatagctat tttgacagtt
    59461 tattaacaat ctgcgtgggg attggcgttt tgccggaggg ggaattgatt attgtagagg
    59521 cccaaaaaac aaaaaccccg gactctcatc cagggttctc tgcttaatag cggaaattac
    59581 gcttcaatag cagcgcgcaa ttttttcatc gcgttctttt ccagctgacg cacacgctca
    59641 gcggaaacac cgtaacggtc agccagttcc tgcaacgtgg acttgttgtc ttcgtccagc
    59701 cagcgcgcgc ggatgatatc ctggctacgc tcgtccagac cttgcatcgc gtcggtcaga
    59761 cggtttgccg cctgctcttc ccagttatca tcttcgatgc cgtcggcaaa gttagatgat
    59821 ttatcctgca gatagagcac cggagccatc ggctggctgt cggaatcgtc gtcggaagac
    59881 aggtcaaagg tcatgtcctg tgccgccata cgtgattcca tctcacgtac gtctttgctg
    59941 gttacgccca gttcacgggc caccatctcg acttcatcct ggttaaacca gcccagacgc
    60001 tgcttggttt tacgcaggtt gaagaacagt ttgcgttgcg ctttggtggt cgcaactttg
    60061 acgatacgcc agttacgcag aacgtattcg tggatctctg ctttgatcca gtgaacggcg
    60121 aaggagacca ggcgcacacc cacttccggg ttaaaacggc gcactgcttt catcaggccg
    60181 atgttacctt cctgaatcaa atccgcctgt ggcaggccat agcccgcata attacgagca
    60241 atatgaacaa caaaccgcag gtgagacagg atcagcgttt tagctgcttc cagatcgcca
    60301 tggtaatgca gcttttcagc cagcgcccgc tcctcgtcag ccgacaacat cggccacgcg
    60361 ttagctgccc ggatgtagga atccaggttg ccaactgggg ctaaagctaa actttgcatt
    60421 ttgtcagtca ttcaaatcct ctcaatcgat atcttctggc gcttcagtgg tagcaacaac
    60481 tgtgccagag cttaagagca acgaggttat cattcactgt tttatcagac cgtgatttta
    60541 tccacaagtt caatgcaagt ttgtgaataa attacgcaca aaatgtgaca tagagataaa
    60601 ataccgggaa gagacaacgg ggtctctttc cctgctacgg aatccattgc agggaaagag
    60661 tataacacgc ttttattatt caggcgtaaa gtggcgtaaa tgttgtaccg tggcaagcca
    60721 cgctgccacc cagccaatca tcgagcatac cagcagcaat agcaggcatt catcgaacga
    60781 taagccattg atatcaaact tcgttccgaa aacctgtgcc acttccgcaa ccgccgatga
    60841 caaacgcagc accagaattt ctgacaaaat tagtgacaac aatgcgccag aaaatcccag
    60901 caatgcgcca ccgtacagga acggacgcag gatgaaacca tccgtcgcac caatcagttt
    60961 ctgcacgtta atagaatcac ggcgagcaaa gatactcagg cgcacactgt taccgatgac
    61021 gaggaatact gccgccacca tcaatacgcc gatcattgcc gaaacgcgcc cgaccagccc
    61081 ggtcaacgcc gccaggcggg caaaccagct gtcatccatc cgcacttcgt caatgccgtt
    61141 aatctgcgtg atacgatcac gcagcgtatt cagtgactcc gtcccctgaa aatcgagttt
    61201 cgggatcacc accgccactg ccggaagcgg gttttcttcc agcatatcca gcgcaccacc
    61261 aaaaccagac cagttacgga actcgcccag tgcgtcttca cgagaaagat aattcacttt
    61321 ctccacgcct tgctcggcct gcaactgtgc caccacgccc gcagcggcgt catcatccag
    61381 cgttttttgc agataaacag tgatttgcgg tgacggataa tattgcgtcg ccgcctggtt
    61441 aacgtttttg tacaccatat aacagacgct gggcagcgtc agggagatgg cgataaccat
    61501 caccgttaaa aacgtggcga acggtttgct tttcagatcc tgcaatgcgc cgtggaaggc
    61561 atagcgtacc tgttcgttga aaacgttggt tttgcgattt accggttttg gcgaggattt
    61621 cgcgcgtttc ggcgcgttac gaccgccgtc gccagagccg ccgaccgatt tacggaagcg
    61681 atccagacgc ccgccaaact gccgaatgtg attgattgca tcgcgcttat tcatggccca
    61741 cgcctccatg caagtgacca tcgctcaggg tgagcatgcg ataggaacgt cgcgaaatca
    61801 ggttgatgtc gtgcgttgcc atcaatacgg ttactccaac gcggttaaac tcttcaaaca
    61861 gacgtaaaat gccttccgac agcgcgtcgt ccaggttacc agtcggttcg tccgccagca
    61921 gtaccgcggg cttgttcacc accgcgcggg caatgccaac acgctgttgt tcaccgccgg
    61981 aaagctgaat agggaagttc ttcgctttgt ccagtagccc gactttatcc agcgccgccg
    62041 acacccggcg acgaatgtca tcaccgctgg caccggcgat aatcagcggg atcgccacgt
    62101 tatcatagac agtacggtcc atcagtagat ggtgatcctg gaaaatcatg ccaatctggc
    62161 ggcgcagaaa cggaacttca cggtttttca gacgcgtgat gtcatggccg ctaaaccaga
    62221 ttttcccggc gctgggccgc tcaatcccac agatcagctt caggagggta cttttccctg
    62281 cgccggaatg accggtcaga aacgccatct cacccggctg catatggaac gtaacgccct
    62341 gcagcgcctg tctcccaccg agataagcct tgctgacatg ttcaaagcga atcattgtta
    62401 atcctctcgg gcaaaaagtg cctctataaa gtcgtccgcc ttaaacggac gcaaatcctc
    62461 aatacgttcg ccgacaccaa tgtagcggat agggatacca aactggtcag ccaccgagaa
    62521 aattaccccg cctttcgccg tgccgtccag tttcgttagc gtgatgccgg ttaagccaac
    62581 ggcttcgtgg aacagtttgg cctggcttac cgcattctgc ccggtgctgg catcgatggt
    62641 cagcataact tcatgcggag cttcaacgtc gagtttcttc atcacgcgga cgattttctt
    62701 caactcttcc atcaggtgcg atttgttttg caggcgtccg gcggtatcgg caatcaggac
    62761 gtcgatgtta cgcgctttag cggcctgaat ggcatcgaag ataacagaag cggaatccgc
    62821 accggtatgc tgggcaatca ccggaatatt gttgcgcaga ccccagacct gaagctgttc
    62881 aaccgctgcc gcacggaaag tatcgcctgc cgccagcatc accgatttac cctgctgctc
    62941 aaactgacgc gccagcttac caatcgtcgt ggttttaccc acaccgttga cgcccaccat
    63001 caggatcacg aacggcgttt taccttcaac attcagcggc tcatcgactt tcgccagaat
    63061 ctcgcccatc tcttctttca gcaggccata gagcgcctcg gcatcacgaa gctgcttgcg
    63121 ggatgcgcct tctgtcagat tggtgataat tttacgagtg gtttccacgc ccacatcggc
    63181 gatcaacagc tgttcttcca gctcttcaaa cagatcatcg tcgatttttt taccgcggaa
    63241 caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc gtttcaggcg
    63301 cgcgaagaaa ccttctttgg tcggtttttc ctgctcctga gcgatttctt ccaccggctg
    63361 atcttcttct gccggagaaa ccaccattac cgcctcttct gccacttcgg cagccagcgc
    63421 ctgtgcctcc ggctcttcgt cggtgatttc ttctttagcc gcttcttctt ccgccgcttc
    63481 gacaatctct acggtttccg cttcggcctg ccactcttct ggcgaaaccg cttcggcgtt
    63541 gacgtcttcc ggcaacggca gttcttcacg ttcgatagcc actggctccg gcgtttcttc
    63601 tacgaccggt tccggctgtg caacgacttc cgcttcaggc tgcgcttttt cactttcagc
    63661 aacctgttca gtaacttcca caacgtcggc agcaaaagtt tccgcctcgg cttcagtatg
    63721 cgcctgcggc tgctcttcaa cggcttgttc agaagccttc acaggctctt gctcctgaac
    63781 gatttcttct acaactggtt gttcattctg aacttctgtc tctttttccg gggtctgctc
    63841 tttttgacca aagcccagcc aggaaaaaaa gccacgtttt ttttctttcg ccatttgcga
    63901 ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata gcagctaaaa
    63961 aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa gtatgtgtta
    64021 tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc ggccaaatcc
    64081 gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagt ccaggtctgc
    64141 gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg gtcattgttg
    64201 acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa gcgttatcgc
    64261 gctacgctgc gggggccacg ttgattgaga tggatcgtgc ggtttctcag cagttaatta
    64321 agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac gcgatgtcat
    64381 tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg ttccgccgtg
    64441 gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct gacgaagccc
    64501 tgatttatgt cgaaagcgaa gtcgaaaagg gtctgcccac tgttccggca aactggtcat
    64561 tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacgc gaagcacaag
    64621 gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgtgtttg gggattttta
    64681 atcctcaacc tggtgcatcc cttcccacgc ccgctgaata ttttcgttaa cgtggcgctg
    64741 atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac tttaccgaaa
    64801 gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg cgtgtttgaa
    64861 ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc gcctacaaag
    64921 ctgacaaagc gcgttccttt atagctcagc gtacctttat cgcccactgt cagggcgtgg
    64981 tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg tttgaagctt
    65041 gcctcatagc gcatactggt gcctgccggc gtcacttcct gctggcgcga acggcgatcg
    65101 ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag cggtgccata
    65161 tcattatcag ctttttcccg tcgttgttgc ataaaacgaa acgatgcggc gacgatgatt
    65221 aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc tcatcggaaa
    65281 atgcggaaat aagcataccc tgccagttat ggtgttgtca tccgtccacc ctcgccacta
    65341 aactggaagc aagaccgtag gcattccgct tacgaaaaaa taacgaattc aaggaactaa
    65401 gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg atgcatcgta
    65461 tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc tcctgctgtt
    65521 gctggcctgg caagcgccga tgttcgacgc cattagctat ctggtgctgg cggggctgtg
    65581 tgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga tgtacgccat
    65641 cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc agatgacgct
    65701 ctctttcttc tggcctctgc cactggtgct gctggttctg ggcgcgctgt tactggcgat
    65761 tatctggacg cgcctggaag agtaccgttg gcctatctgc acgtttatcg gcatgacgct
    65821 ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc cggcgctctc
    65881 tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg gtagtcacta
    65941 tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg ctggtcactt
    66001 cctcatcgtc cgctcgctgt atctctgata aaacttgact ctggagtcga ctccagagtg
    66061 tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgact cctgacaatc
    66121 acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aactacggta cagaacacca
    66181 acgactgttg ctgcgacggc gcatgttcca gcacgccaac tctctctgaa aacgtctccg
    66241 gcacccgcta tagctggaaa gtcagcggta tggactgcgc cgcctgtgcg cgcaaagtgg
    66301 aaaatgccgt gcgccagctt gcaggcgtga atcaggtaca ggtgttgttc gccaccgaaa
    66361 aactggtggt ggatgccgac aatgacatcc gggcacaagt tgaatctgcg gtgcaaaaag
    66421 cgggctattc cctgcgcgat gaacaggcct ccgacgaacc acaagaatca cgcctgaaag
    66481 agaatctgcc gctgattacg cttatcgtga tgatggcaat cagttggggg ctggagcagt
    66541 tcaatcatcc gtttggtcaa ctggcgttta tcgcgaccac gctggttggg ctgtacccaa
    66601 ttgctcgtca ggcattacgg ctgatcaaat ctggcagcta cttcgccatt gaaaccttaa
    66661 tgagcgtagc cgctactggg gcgctgttta ttggcgcaac ggctgaagct gcgatggtgt
    66721 tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagtcgc gcgcgtcaag
    66781 gggtcagcgc gttaatggcg ctgaagccag aaaccgccac gcgtctgagt aacggtgagc
    66841 gtgaagaggt ggcaataaac agcctgcggc ctggcgatgt gattgaagtt gcggcaggtg
    66901 ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat gaaagtgccc
    66961 tgaccggcga atccattccg gtggagcgcg cgacgggcga taaagttcct gcaggcgcca
    67021 ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc agcgccattg
    67081 accggattct gaaactgatt gaagaagccg aagagcgccg cgcacctatt gagcggttta
    67141 tcgaccgttt cagccgtatt tacacgccag cgattatggc ggtcgccctg ctggtaacgc
    67201 tggtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa gggctgacgc
    67261 tgctgctgat tggttgcccg tgtgctttag ttatctccac gcccgcggcg attacctccg
    67321 ggctggcggc agcagcgcgt cgtggggcgt tgattaaagg cggcgcggcg ctggagcagc
    67381 tgggtcgtgt tacccaggtg gcgtttgata aaaccggtac gctgaccgtc ggtaaaccgc
    67441 gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg acactggcgg
    67501 cggcggtcga gcaaggcgcg acgcatccac tggcacaggc cattgtgcgc gaagcacagg
    67561 ttgctgaact cgccattcct accgcccaat cacagcgggc gctggtcggg tctggcattg
    67621 aagcgcaggt taacggtgag cgcgtgttga tatgcgctgc cggaaaacat cctgctgatg
    67681 catttgctgg tttgattaat gaactggaaa gcgccgggca aacggtagtg ctggtagtac
    67741 gtaacgatga cgtgctgggt gtcattgcgt tgcaggatac cctgcgcgcc gatgctgcaa
    67801 ctgctatcag tgaactgaac gcgctgggcg tcaaaggggt gatccttaca ggcgataatc
    67861 cacgcgcagc agcggcaatc gccggggaac tggggctgga atttaaagcg ggcctcttgc
    67921 cggaagataa agtcaaagcg gtgaccgagc tgaatcaaca tgcgccgctg gcgatggtcg
    67981 gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt gcaatgggta
    68041 gcggcacaga cgtggcgctg gaaaccgccg atgcagcatt aacccataac cacctgcgcg
    68101 gtctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc cagaacatca
    68161 ctattgcgct ggggctgaaa ggcgttttcc tcgtcacgac gctgttaggg atgaccggac
    68221 tgtggctggc agtgctggca gataccggtg cgacggtgct ggtaacggcg aatgcgttaa
    68281 ggctgttgcg caggaaataa ggcaaaccga tcgcaacatt gagcgcgatc ggtcccctcg
    68341 ctcctctggg gagagggtta gggtgagggg aaaaggcggc atcgaagcca atcttccccc
    68401 atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct ttagcaacca
    68461 gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtcgtcgcc ggatcgtcag
    68521 caataatcag caacgtttca cctggctgca tattgcgcac tgttttgcgc accatcatta
    68581 ctggttccgg gcagcgcagg ccaagtgcgt cgagtgtgtg gtcagggctg gaaaagagat
    68641 cggtcatttt cttctcatca cataaaaaaa cggcgctagt ttacgccctg tgagtccgta
    68701 agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg taaagcagta
    68761 tcatgcggcg gctcgaaaaa agggtcagca cgtataaaat agcgtgtcgc acgtattggg
    68821 ttccctcacc ccaatggtta atcaaaaagg tacaatatga acgttttctc gcaaacgcaa
    68881 cgctataagg cgttgttctg gttatcgtta tttcatcttc tggtgatcac ctccagtaac
    68941 tatctggttc agcttccggt ctccattttt ggttttcata ccacctgggg cgcgtttagc
    69001 tttccgttta tctttctcgc tactgacctg accgtgcgta tttttggcgc gccgctggct
    69061 cgtcgcatca tctttgcggt aatgatcccc gcattgctga tctcctacgt catctcatcg
    69121 ctgttttata tggggtcatg gcaaggattc ggcgcgctcg cccacttcaa cctgtttgtc
    69181 gcccgtatcg ccaccgccag tttcatggcc tacgcgctgg ggcagatcct cgacgtgcac
    69241 gtttttaacc gcctgcgtca gagtcgtcac tggtggctgg caccgacagc gtccacactg
    69301 ttcggtaacg tcagcgacac gctggccttc ttcttcattg ccttctggcg tagcccggat
    69361 gcctttatgg ctgaacactg gatggaaatc gcgctggtcg attactgttt caaagtgtta
    69421 atcagtatcg ttttcttcct gccaatgtat ggcgtattac tcaatatgct gttgaaaaga
    69481 ctggcagata aatccgaaat caacgctttg caggcgagtt aaaggttcgt tatcagagtt
    69541 gtgataagat ggatgaatga gccgttatgg ccgtttatcg aaaggaagaa gtcaatgcgc
    69601 aatctggtta aatatgtcgg aattggcctg ctggttatgg ggcttgcggc ctgtgatgat
    69661 aaagacacta acgctacggc gcagggttcg gtcgcagaaa gtaacgcgac cgggaatccc
    69721 gtcaacctgc ttgatggcaa gttaagtttc tcgctgccag cggatatgac cgaccagagc
    69781 ggtaagctgg gaacgcaggc caataacatg catgtctggt ccgacgccac cgggcagaaa
    69841 gcagtcatcg tcatcatggg cgatgatccg aaagaagatc tggcggtgct ggcgaagcgt
    69901 ctggaagatc agcaacgtag ccgcgatccg cagctgcaag tggtaaccaa taaagccatt
    69961 gagctgaaag gtcacaaaat gcagcagtta gacagcatta tctccgcgaa aggccagacg
    70021 gcgtactctt ccgttattct gggtaacgtg ggtaatcaac tgctaaccat gcaaattacg
    70081 ctgcccgctg acaatcagca aaaagcacag accaccgcag aaaacatcat taatacgctg
    70141 gttattcagt aagttttaag atgatgaggc ggcctcaggt atttccaccg ggggccgttt
    70201 ttttaatcgc cacgtcagta ataacgcgat tgcgaccagt cccgccgccg ccagataaat
    70261 taccggaacg cccgcccaac tcatcaccag cccagccagt ggtccggtca cgccaagcga
    70321 taaatccata aatacggtgt aagttgccag cgccgccccc tgattttgct gcggaaccgc
    70381 ttttaccgcc actacgccca atgcagggaa caccagcgaa aaccccgccc ccgccagtaa
    70441 gacgccgatt ttcgccatcc acggcatagt cgccacgcca accagtagca ggccgattat
    70501 ctcaacgcta aagcaaatca tcgccacgtt taagccgccg atacggttaa tgccgttagg
    70561 gaataacaaa cgcgtaccga caaacgcaca gctaaacagc gtcagcgcga aagccgcacc
    70621 gtcccaacct ttagcgtcat aaaacaacgt gataaaggtg gcgataacgc caaatccggc
    70681 ggaagccagt gccagcgcca taccgtacag ccagacgcgc ccaagcaccg cacgaaacgg
    70741 cagcggtttg cctttactgg cttttaccgt cggacgcggg atcgccaaca aaatggccac
    70801 cagtgccacg cccataatga ttaacgctaa cgcctgcaat ccgccccagt gataaaacac
    70861 gacacctaac ggcgcaccca tcgccatcgc cccgtaagtg acaataccgt tccacgaaat
    70921 cacccgcccg atatgtagcg aaccaaccac gccaacaccc cacagggtcg atcccgttcc
    70981 ggcaaaactt tgtccaatcc caaggattac gcgccccagg cacagtaata acaggctgat
    71041 gacgggcaga ctggcggtta atcctgccgt cagatatccc agaccgctca taaagcagcc
    71101 gcataaaccg aagacgacaa tctttttggg tcccagcaaa tcggcgtaac gtccggcatg
    71161 aggacggctc agcaaggtgg cgaaatattg caggctgata accaaccctg cccagaaggc
    71221 actaaagccc atcacatcat ggacatagcc tggtaataca gcgagcggca acccgatggt
    71281 gaggtagctg gcgaagttaa acatgacaat ggagacaatg cgcaaattca ggcgcaatcc
    71341 gtttagcgcg ggttcggcta cgggttcggg catgaggatc accacatttt tacaacagtg
    71401 tttcattttt accatgtgct gacgtgaaaa tcagcagtaa gaatcagaat attgctggcg
    71461 tgactcccgc tacacttaat acaaaaagtc acaaggaagc cccaatggaa acccctcaac
    71521 ccgataaaac gggcatgcgc attctgctca agttggcctc gctggtagtg atcctcgcgg
    71581 gcattcacgc agcggcagat atcattgtgc agctattact ggcgctgttt tttgccatcg
    71641 ttctcaaccc gctcgtcacc tggtttattc gtcggggagt acaacgcccc gttgccatta
    71701 cgattgtggt tgtggtgatg ctgatcgcac tcaccgcgct ggtcggcgta ctggcggcat
    71761 cgtttaacga atttatctct atgctgccga agtttaataa ggagctgacg cgcaaacttt
    71821 ttaaattgca ggagatgttg ccttttctta atttgcatat gtcgccggag cgaatactac
    71881 agcggatgga ctcggaaaaa gtggttacct tcaccacggc gctaatgacc gggctttccg
    71941 gggcaatggc gagcgtgctt ttgctggtga tgaccgtagt ttttatgctg tttgaagtgc
    72001 gccacgtccc ttacaaaatg cgtttcgcgt tgaataatcc acagattcac atcgccggac
    72061 tacaccgcgc acttaaaggc gtttcgcatt atctggcgtt aaagacgcta ctcagtttat
    72121 ggacaggcgt aatcgtctgg ctggggttgg agctgatggg ggtgcagttt gcgctgatgt
    72181 gggcagtact ggcgtttttg ctcaactacg tgcccaatat cggtgcggta atttccgccg
    72241 taccgccaat gattcaggtg ctgctgttta atggtattta cgaatgtatt ctggtcggcg
    72301 cattgttttt agtggtccat atggtcatcg gcaatatttt agaaccacgg atgatgggcc
    72361 atcgcctggg gatgtccacc atggtggtat ttctttcatt gttaatttgg ggatggctgc
    72421 tcggcccggt agggatgcta ctttcggtgc cattaaccag cgtgtgtaaa atctggatgg
    72481 aaaccaccaa aggcggcagc aaactggcga ttttactggg gccaggcaga ccgaaaagtc
    72541 ggttaccggg atgaggcgac aagtgttacg atacgcactt tcattttcca ttaaacgttg
    72601 gccctgatat gtatcggata gttctgggga aagtttcgac cttaagcgca gctcctctgc
    72661 cactgggttt acgcgagcaa gcaccgcaag gtccacgacg cgaacgctgg ctggcggggc
    72721 gtgcattgct ttcgcacacg ctttccccgc taccggagat catctatggc gaacaaggca
    72781 aacctgcatt cgcgccggaa acgccgctat ggttcaactt aagccatagc ggtgacgata
    72841 tcgccctgct gttgagcgat gaaggcgaag tcggctgcga tatcgaagtg attcgcccgc
    72901 gcgccaactg gcgctggctg gcgaacgccg tattcagcct cggggaacat gccgagatgg
    72961 acgccgtgca tcctgatcag caactggaaa tgttctggcg catctggacg cgcaaagaag
    73021 ccatcgttaa acagcgtggc ggcagcgcct ggcaaatcgt cagcgtagac agcacctata
    73081 actcctcgct gtcagtcagc cattgtcagc tagaaaattt aagcctggcg atctgcaccc
    73141 ctaccccctt tacgctcacc gccgacagtg tgcaatggat cgattcagtt aactgatccg
    73201 cccacccgac tgcccatcta ttgatccaga acaggtaatc agtatgacga atacttaaaa
    73261 tcgtcatact tatttccgcc atctatttta atccattggg gttaccatgt tctctacact
    73321 ccgccgcact ctatttgcgc tgctggcttg tgcgtctttt atcgtccatg ccgctgcacc
    73381 agatgaaatc accaccgcct ggccggtgaa tgtcgggcca ctaaacccgc acctttacac
    73441 gcctaaccaa atgttcgccc agagcatggt ttatgaaccg ctggtgaaat atcaggcaga
    73501 cggttcggtg atcccgtggc tggcaaaaag ctggacccat tcggaagatg gcaaaacctg
    73561 gaccttcacc ttgcgtgatg acgtgaaatt ctccaacggc gaaccgtttg atgcagaggc
    73621 ggcggcagaa aacttccgtg cagtgctcga taaccgtcaa cgtcacgcct ggctggagct
    73681 ggcaaaccag attgttgatg tcaaagcact cagcaaaaac gagctgcaaa tcaccctgaa
    73741 aagcgcctac tatcctttcc tgcaagaact ggccctgccg cgtcctttcc gctttattgc
    73801 cccgtcacaa tttaaaaatc acgaaaccat gaacgggata aaagcgccga ttggcaccgg
    73861 accgtgggtt ttgcaggaat cgaaactgaa tcagtacgat gtcttcgtcc gtaacgaaaa
    73921 ctactggggc gaaaaaccgg cgattaaaaa gatcaccttt aacgtgatcc cggacccgac
    73981 tacccgcgcg gtggcgtttg aaactggcga tatcgacctg ctgtacggaa acgaagggtt
    74041 attaccgctc gataccttcg cccgctttag ccagaacccg gcttaccaca ctcaactgtc
    74101 acagccgatc gaaaccgtga tgctggcgct caataccgcc aaagccccca ccaacgagct
    74161 ggcagtacgt gaagctctta attacgcggt gaacaaaaaa tcgctgattg ataacgcgtt
    74221 gtatggtacc cagcaggtcg ccgacaccct gtttgcccct tctgtgccct acgccaacct
    74281 cggcctgaaa ccgcgccagt acgatccgca gaaagcgaaa gcgttgctgg aaaaagccgg
    74341 ttggacgctg cctgctggca aagacatccg cgagaaaaat ggtcagccgc tgcgcattga
    74401 actgtcgttc atcggcaccg atgcgttaag caaatcgatg gcggaaatca ttcaggctga
    74461 tatgcgccag attggcgcag atgtctcgct gattggcgaa gaagagagca gtatctatgc
    74521 tcgtcagcgc gacggtcgtt ttggcatgat tttccaccgc acctggggcg cgccatacga
    74581 tccacacgcc ttcctgagtt caatgcgcgt accgtcgcac gctgacttcc aggcacagca
    74641 aggtttagcc gacaagccgc tgattgataa agagatcggc gaagtgctgg cgacccatga
    74701 cgaaacgcaa cgtcaggcgc tgtatcgcga cattctgacc cgtctgcatg acgaggcggt
    74761 ttatctgcct atcagttaca tctcaatgat ggtggtttca aaaccggaac tgggtaacat
    74821 cccctacgcg ccgatcgcca ccgaaatccc gttcgaacag attaaaccgg tgaaacctta
    74881 atgttgcgtt atgtattacg ccgctttctg ctgctgatcc cgatggtgct cgccgcctcg
    74941 gtgatcattt ttttgatgct gcgcctcggc accggcgacc cggcgcttga ttatttgcgt
    75001 ttatctaacc tgccgccgac gccggagatg ctggcctcta cccgcactat gctggggctg
    75061 gatcagccgc tgtacgtcca gtatggcacc tggctatgga aggcgctgca tcttgacttt
    75121 ggtatctcat tcgccagcca acgcccggtg ctggacgata tgctgaactt cctgcccgcc
    75181 acgctggaac tggcgggtgc ggcgctggta ttaattctgc tcacctccgt gccgctcggt
    75241 atctgggcgg cgcgccaccg cgatcgcttg cccgatttcg ccgtccgttt catcgcgttt
    75301 ctcggcgtgt cgatgcctaa cttctggctg gcgtttttac tggtgatggc gttttcggtg
    75361 tatctgcaat ggctacccgc gatgggttac ggcggctggc agcacatcat tttgcctgcg
    75421 gtatccatcg cctttatgtc gctggcgatt aacgcgcgtt tgctgcgcgc cagtatgctg
    75481 gacgtcgccg gtcagcgtca tgtgacctgg gcgcggctgc gcggcctgaa tgacaaacag
    75541 accgaacgcc gccatattct gcgcaatgcc tcgctgccga tgatcactgc cgtggggatg
    75601 cacattggcg aactgattgg cgggacgatg attatcgaaa acatctttgc ctggccgggc
    75661 gtcgggcgct atgcggtgtc ggcgattttt aaccgtgact atccggtgat ccagtgcttt
    75721 acgctgatga tggtggtggt ttttgtggtc tgtaatttga ttgtcgattt gctcaacgcc
    75781 gcgctggacc cgcgtattcg tcgtcatgaa ggagcgcacg cgtgaacttt ttcctctcct
    75841 cccgctggtc ggttcgcctg gcgctgatca ttatcgccct gctggcgctg attgcgctca
    75901 ccagccagtg gtggctgccg tatgaccctc aggctattga tttgccgtcg cgcctgctct
    75961 cgccggatgc gcagcactgg ctgggcaccg atcatttagg gcgcgatatt ttctcgcggc
    76021 tgatggcagc gacccgcgtg tcgctcggct cggtaatggc ctgcctgctg ttggtgctga
    76081 cattagggct gattattggc ggcagcgccg ggctgattgg cgggcgcgtt gatcaggcca
    76141 ccatgcgcgt cgcggatatg tttatgacct tcccaacctc gattctgtcg ttctttatgg
    76201 tcggcgtgct tggcaccggg ttgaccaacg taattatcgc tatcgccctt tcgcactggg
    76261 catggtacgc gcgcatggtg cgcagtctgg tgatttcact gcgccagcgc gagtttgtgc
    76321 tggcatcacg gctttctggc gcgggccatg tgcgggtgtt tattgatcat ctggcaggtg
    76381 cggtgatccc ctcgctgctg gtgctggcga cgctggatat cggtcatatg atgctacacg
    76441 tcgcggggat gtccttcctc ggtctcggcg tgaccgcgcc gaccgccgaa tggggcgtga
    76501 tgattaacga cgcgcgtcag tatatctgga cccagccgct gcaaatgttc tggccggggc
    76561 tggcgctgtt tatcagcgtg atggccttta acctggtggg tgacgcactg cgcgatcatc
    76621 tggaccctca tctggtgacg gagcacgcac actaatgcca caacagattg aactccgtaa
    76681 tatcgcgcta caggccgcgc agccgctggt gcacggcgta tcgttaaccc tgcaacgtgg
    76741 gcgtgtgctg gcgttagtcg gcggtagtgg cagcgggaag tcgctgacct gcgccgcgac
    76801 gctgggcatt ctgcccgcag gcgttcgcca gacggcgggg gaaattttgg ccgatggaaa
    76861 accggtttct ccctgcgccc tgcgcggcat caaaattgcc accatcatgc agaacccgcg
    76921 cagtgccttt aatccgctgc acaccatgca cacccacgcg cgtgaaacct gcctggcgct
    76981 ggggaaaccc gccgatgacg ctacgcttac cgctgccata gaagcggtgg ggctggaaaa
    77041 cgccgcgcgc gtgctgaagc tctacccgtt cgagatgagc ggcggcatgt tgcagcgcat
    77101 gatgattgcg atggcggtgc tgtgcgaatc accgtttatc atcgccgatg aaccaaccac
    77161 cgatctcgac gtggtagcac aagcacgcat cctcgatcta ctggaaagca ttatgcaaaa
    77221 acaagcaccg ggaatgctgc tggttaccca tgatatgggc gtagtagcgc gtctggcaga
    77281 cgatgtggcg gtaatgtctc acggtaaaat tgtcgaacag ggcgatgtag aaacgctgtt
    77341 taacgccccc aaacatgcgg taacgcgcag cctggtttcc gcgcatctcg ccctctacgg
    77401 tatggagctg gcatcatgac tttacttaac gtctccgacc tttcccatca ctatgcgcac
    77461 ggtggattta gcggaaaaca tcagcaccag gcggtgctga ataacgtttc cctggccctg
    77521 aaaagcggtg aaaccgtcgc cctgctggga cgcagcggct gcggaaaaag taccctcgcg
    77581 cggctgctgg tagggttaga gtcgccctca cagggaaata ttagctggcg cggcgaaccg
    77641 ttggcgaagc tcaatcgcgc gcaacgtaaa gcgttccgcc gtgatattca aatggtgttt
    77701 caggactcca tcagcgccgt gaatccgcgc aaaaccgtgc gcgagatcct gcgtgaaccg
    77761 atgcgccacc tgctatcgct gaaaaaagcc gaacaactgg cgcgcaccag cgaaatgctg
    77821 aaggcggtcg atctcgacga cagcgtgctc gacaaacgcc caccgcagtt aagcggcggc
    77881 cagctccagc gtgtctgtct ggcacgcgcg ctggcggtcg aaccgaaact gctgattctg
    77941 gatgaagccg tttcgaacct tgatctcgtg ttacaggcgg gcgtcattcg cctgctgaaa
    78001 aagctgcaac aacagtttgg caccgcctgc ctgttcatca cccacgactt acgcctggtg
    78061 gaacgcttct gtcagcgggt gatggtgatg gacaacggac aaatcgtcga aacccaggtg
    78121 gtgggagata aattaacctt ttcctctgac gccggacgtg tgctacaaaa cgcggtatta
    78181 cccgcattcc ccgtgcgccg tcgcaccaca gaaaaggttt aacgcaaatg caacgagtca
    78241 ccatcacgct tgatgacgat ttactggaga cgctggacag cctgagccag cgtcgtggtt
    78301 ataacaaccg ttccgaggcg atccgcgata ttctgcgtag cgctctggcg caggaagcca
    78361 ctcaacaaca cggcacacaa ggtttcgcgg tgctgtcgta tgtgtatgaa cacgaaaaac
    78421 gtgacttagc cagccgtatt gtctccaccc agcatcatca ccacgatctc tcggttgcta
    78481 ctctgcatgt gcacattaat cacgacgact gtctggaaat cgccgtgttg aaaggtgaca
    78541 tgggtgacgt acaacatttt gccgacgacg tcatcgccca gcgcggcgtg cggcacgggc
    78601 atttgcagtg cctgccgaag gaagattaac acctcccaac gcgccgttat ttcctgacgg
    78661 cgcagtagac tcctgccaaa tatcgactca cgtcacattt ttttgatcac gatttggtcc
    78721 cctaaaatgt gagacgcttc gatcctcttt ttcgcctgtt cacctactat acgcttgaag
    78781 ttgtccatga ttgtccatag acaactgtaa atgttcaggt gcgcgccatg aaatcattga
    78841 gtaagtcgtc acaaataccg ctctatcaac aagtggtgga gtggataaga gaaagtattt
    78901 ataccgggga tctggtggaa gacgatcgca ttccttcgga ataccagatt atggatatgc
    78961 tggaagtgag tcggggaacc gttaaaaaag cggtcgccca actggtaaaa gaaggcgtgt
    79021 tgattcaggt tcaggggaag ggaacatttg tcaaaaaaga gaacgtggca tatccgttag
    79081 gtgaaggatt attgtcattc gcggaatcgc tggaaagcca gaaaatacat tttaccactg
    79141 aagttattac gtcacggatt gaaccggcta atcgttatgt ggcagagaaa ttaagaataa
    79201 cgcccggtca ggatattctt taccttgagc gtttacgttc aattggtgat gaaaaagcga
    79261 tgctgataga gaaccgtatc aatattgagc tatgccccgg catcgtggaa atcgatttta
    79321 atcaacacaa tttatttcca acaatagaaa gtttgtcgca aagaaaaatt cgttactcgg
    79381 aaagtcgcta tgccgcgcga ttaattggta atgaacgcgg tcatttttta gatatcagtg
    79441 aagatgcacc cgttttgcat ctggagcagt tagtcttttt ctcccgcgga ttacccgttg
    79501 agtttggcaa cgtctggtta aaaggaaata aatactatct tggcaccgtg ctgcaacggc
    79561 gggaagtgag ttaagtacac ttttttaaga ggaataaaat gcaaggcata cagtttcagg
    79621 aaaattttat tcaacgactt ccggcagggt taagcgttga acagatcatc cgtcagttag
    79681 cgcaaccgtt ggttaccgcc gaactggtgg tccctgattt tgccgatcat gtactggaac
    79741 gtgaggcgac gtacccaacg ggactgccta ccgaaccacc gtgtgtcgcc attccgcaca
    79801 cggaccataa acatgtcagg cacaatgcaa ttgccgtcgg catcctgccg gaaccggtag
    79861 tgtttgccga tatgggcggc gaccctgatc ccgtacctgt cagggtgatt tttttgctcg
    79921 ccttaggcga aagtaacaaa caattaaatg ccctggggtg gattatggag atgattcagg
    79981 atacgccctt tatgtgcgcc ctgcttacga tggaaacaac agaaatacac acagcaatct
    80041 taaacaaaat gaaagaacga ggtgaaatat gagtcaaacc attttatttg tctgcgccac
    80101 gggtattgcc acatccacgg cagtaacaga aaaagtcatg gaatattgta aagagcacgg
    80161 tcttaacgtt aattattctc aaaccaacgt tgcctcttta cctggaaata ccgacggcgt
    80221 agcactggtt gtttcaacga ctaaagtgcc ttatgaactc gacgtgccgg tggttaacgg
    80281 cttgccaatt attactggta ttggcgaaga gaaagtattg gcgcaaattg tttctatcct
    80341 aaaaaagtaa ttaataataa ttaatttcaa cacactacat caggcgctaa cgcgaggtgc
    80401 gctctcgcct gaaaaagagg tttattatga acgatatcgc gcataccctc tataatattg
    80461 tgcaatatat attgggattt ggtcccacgg taatgttgcc gttggtgtta tttattcttg
    80521 ctctcttttt caaagtaaaa cccgctaaag ccttacgttc gtcattaacg gtcggcattg
    80581 gttttgtcgg tatttatgcc attttcgata ttcttaccag caatgtcggg ccagcggccc
    80641 aggcgatggt tgaacgcacc ggaattaatt taccggtggt ggatttaggc tggccgccgc
    80701 tttccgctat tacatggggt tcgccaattg ccccgtttgt tattcccctg actattctga
    80761 ttaacgtggc gatgctggcg ttaaataaaa cccgcaccgt tgacgtagat atgtggaact
    80821 actggcattt tgcccttgcc ggtacgctgg tttattacag ctccggcagc ctgttctttg
    80881 gtttgctggc ggcggcgatt gctgcggtgg tagtacttaa actcgccgac tggtctgcgc
    80941 cactggtaca aaaatacttt ggcctggaag ggatctcttt gccgacgctc tcttcggtgg
    81001 tgttctttcc ggtcggtctg ttggtcgaca aaatcatcga ccatatcccc ggcctcaatc
    81061 gtattcatat cgatccggaa accgtacaga aaaagtttgg cattttcggc gaaccgatga
    81121 tggttggcac tattctgggc atttttctcg gcgtaattgc cggatacgat ttcaaaaaag
    81181 tattgctgct tggcatcagc attggcggtg tgatgttcat cctgccacgc atggttcgca
    81241 ttctgatgga aggtttatta ccgctgtctg aagccattaa aaagtatctc aatgccaaat
    81301 accctgaccg tgacgatctc tatatcggcc tggatatcgc cgttgccgta ggtaatccgg
    81361 cgattatctc caccgccctg ctgctaacgc caatctcggt ctttatcgcg tttgtcctcc
    81421 caggtaatga agtcctgccg cttggcgacc ttgccaacct ggcggtaatg gcgtcgatga
    81481 ttgctttagc cagccgtggc aacattttcc gcaccgttct ggcggcgatc ccggtgatta
    81541 ttgccgacct gtggattgct accaaaatcg cgccgtttat taccggaatg gcgaaagacg
    81601 ttaacttcaa atttgccgaa ggctccagcg gccaggtttc cagtttcctt gatggcggta
    81661 acccgttccg cttctggctg ctggaaatct tcaacggcaa tctcatcgcc attgctctgg
    81721 tgccggttat tgccctggtg ctgtatggca ttttccgaat gacgcggagc acggtttatg
    81781 cctgataaca gcgccgctat cgttatcgat attggcacca ccaattgcaa agtcacctgc
    81841 ttttcctgcc tggacgcaac gacgttgggc gcgcataaat tcgtgacggc aaaacagatc
    81901 tccccacagg gcgatgtcga tttcgatatc gacgccctct ggcaggaggt ccgccaggcg
    81961 atagcgcaac tgaacgccgc ttcaccgctg cctgtcaggc ggatcagcat tgccagtttt
    82021 ggcgagtcag gggtgtttct tgacgaacat ggcgagatcc tgacgccaat gctggcatgg
    82081 tatgaccgtc gcggtgaaga gtatctggca acgcttagcg aggcagacag tgcggcactt
    82141 tatgacattt gcggattacc actgcacagc aattactctg ccttcaaaat gcgttggttg
    82201 ctggagcatt atccgctgcg caatcgtcgc ggcctgcgct ggctacatgc accggaagtg
    82261 ctgctctggc ggctgactgg cgaacagcga acggatatca ccttagccag ccgtacactg
    82321 tgtctggacg tgcgcaaagg cgaatggtca gcgaaagcgg cggcgttgtt acacgttccc
    82381 tgttcagcat ttgcgccatt ggtgcagcca ggcgaacacg ccggatgggt cagcgagtcg
    82441 ctctgcaaaa cgcttgggtt ctcgcaaccg gtcagcgtga cgctggccgg acatgaccat
    82501 atggtgggtg cgcgggcgtt gcagatgatg ccgggcgata tccttaactc gacgggcacc
    82561 acggaaggca ttctgcaact ggatacacaa ccgacgctgg atgaacaggc caaacgtgac
    82621 aagctggcaa acggctgtta ctcgcttgcc aaccagttca ccctgtttgc ttctctgccc
    82681 gtgggcggtt tcgcgctgga gtggctgcgc aacacgttcc ggctaactga tgaggagatc
    82741 gccgcgtcgc tcaatcgtgg gtatgcggat tacctggcgg ggaactggtc gcttgatgac
    82801 attcccgtct ttattccgca tcttcgcggt tcgggttcac cctataaaaa tcgccacacc
    82861 cgcggattat tttatgggct tggcgatacg ttaaatatcg acatgttaat ttccagcgtc
    82921 tcactgggat taaccatgga atttgccaac tgctttgcct gttttaatgt gcctggcacc
    82981 agcgtgttaa aagtgatcgg tccggcgacc cataaccccc tttggctgca attaaaggca
    83041 gatattttac agcgtccggt tgaagcaatt gcatttagcg aggcggtttc tgtcggagcc
    83101 ttattaaccg cggcaccgga tattccgccg ccgcaagtta ccatagccca acgtttgtta
    83161 ccgaatcggg caagatatca tcaattacag cgttatcagc acaaatggaa aagctggtat
    83221 cagttgaaat tacagcaaga aggcgtgatg ccattacatc atcgggagga gcactatgtt
    83281 gactaaaacc gtggaggtca gaaacagtac tggcctgcat gcacgtccgg cggcctgtct
    83341 ggcgaaagcc gccaaaaaat gtagctgtaa agtgacgctg cactatgagg gtaatgatat
    83401 taatgccacc agtatgatga atattatgcg cgccggaata aaaggcggaa aaacggtgga
    83461 aatacgctgc gacggtgatg atgaaaacga ggccatacag actctcaccg ccttatttcg
    83521 cgaccgcttc ggtgaagctg aataattaac tgatattaaa aaatgaggtt ttcaatatgc
    83581 cattagtgaa tggacggata ctgctcgacc gcattcagga aaaacgggta ttagcgggcg
    83641 cgtttaatac caccaatctg gagacgacaa tttcaatttt aaacgctatt gagcgttcgg
    83701 gattacccaa cttcattcag attgcgccca ccaatgcaca actgtcggga tacgattaca
    83761 tatatgaaat cgtgaaacgt cacgccgata aaatggatgt tccggtcagt cttcatctgg
    83821 atcacgggaa aacccaggag gatgttaaac aggcggtgcg cgcgggcttt acctcggtca
    83881 tgattgacgg tgccgcattc tcttttgaag agaatatcgc tttcacccag gaagctgtcg
    83941 atttctgtaa atcttatggc gtgccggttg aagcggagct gggggcgatt ctcggcaaag
    84001 aagatgatca cgtcagcgag gccgattgca aaacggaacc ggaaaaggtg aagacctttg
    84061 tcgaacgcac cggttgcgac atgctggcag tttctattgg taacgttcat ggcctggacg
    84121 atataccgcg gatcgatatc cccctgctga agcgcattgc cgaagtcagc ccggtgccgc
    84181 tggttatcca cggcggctcc ggtatcgccc cggagatatt acgcagtttc gttaattatc
    84241 gcgtcgctaa agtgaatatc gccagcgatc tgcgcaaagc gtttatcacc gcagtcggca
    84301 aagcgtatgt gaataatcac aacgaggcga atctggcacg agtgatggca tcagcaaaaa
    84361 acgccgtcga ggaagatgtg tatagcaaaa tcctgatgat gaatgagggg catcggtcgg
    84421 taaaataaat cctcgccgga tgcggcgtga cgccttatcc ggcctacaaa accactagaa
    84481 ttcaataaat tgcaccatcg cgtaggcccg ataagcttgc gtatcgggca ctgtatttac
    84541 gccattgtcc caatcgtctt cctgaatcgc agcagcgcaa tggtaaaaaa cgcgccgcca
    84601 atcgctatca gcgtcaggaa ctgcggccag acgatttcga atccggcacc acgatagagg
    84661 atggcctgcg cgaggctgac aaagtgtgtc gtcggcatgg tcagcataat gtcctgcacc
    84721 atctgcggca tactttcgcg cggcgtggaa ccaccggaaa gcatttgcag cggcagcagc
    84781 accagaatca ccagcagccc cagttgcggc attgaacgcg ctatcgtccc cataaaaatg
    84841 ccgattgacg tggtggcaaa cagactgagc gccacgccca gcataaacag cgggatcgag
    84901 ccttctatcg gtacgcccag cacgcctttc accatcagca ccagcgataa tcccgacacc
    84961 accagcacca ccagccccat cgaccagacc tttgccatca tgatctcaaa cggcgttatc
    85021 ggcatcacca gtaagtgttc caccgtgccg tgttcacgct cgcggatcag cgccgatccg
    85081 gtcaatacaa tcgccagcat ggtaatgttg ttgatgatcg ccatcacccc gccaaaccac
    85141 gcgggatcaa ggttcgggtt aaagcgcatc cgtgtttcca gcgataccaa cggttcgctg
    85201 ttatcacggt agcgcgcgac aaagctgttc acttcaccgt tgataatatt ctggatatac
    85261 ccattgccgg taaatgcctg gctcatgcgc gtggcatcaa cgttcacctg aatatccggc
    85321 tggcgtccgg cgaggacatc acgctgaaaa ttaggcggaa tatttatcgc gaaggtataa
    85381 cgtccggcgt ccagcccggc atccatctca tcggcggtga tcatctccgg tggcaaaaac
    85441 cacgggcgat agaagctgtt aacgatccgg ttcgataact gcgattgatc catatcggca
    85501 atggcgatcg gcgcgaggtt caacgatcct ggcgtaacgg tcgctgacga atacaccgac
    85561 accgtaaacg agaagacaat cagcgtcagc atcgctttat caccgagcag actgcgcaac
    85621 tctttgatac ccagattaaa aatattacgt aaatggcgca tcatccctcc tgttttttca
    85681 gcagcaggat gcttaagccc atcaccagcg gtatggctat cagcaacggg ataaaaagtt
    85741 gccacaaatc agtcagatcc agcgctttcg agaacgttcc gcgggcgata gtcagaaaat
    85801 gactggtcgg gtaaacctcg ccgatccaac gtcctggacc ttccagcgaa gctaccggat
    85861 cgatcatccc ggaaaactgt gtcgccggga tcaacgtgat aattgccgta ccgaaaatgg
    85921 cggcaatctg gcttttcata aaggtggaga tcagcagccc cattccggtg gcgatgatga
    85981 tatacagcag cgccgccagg gtgagcgtca ggaaactgcc tttatgcggt acgccaaaca
    86041 caaacaccga cagggcgcag agcaggaaaa agttcagcat ccccagcgag atgtatggca
    86101 gctgtttgcc aagtaaaaat tcgctacggg tggtgggggt cacgtaaagg ttgataatcg
    86161 accctagctc tttttcccgc accacgctaa gggcgcttag cattgacggg atcatcatca
    86221 gcagaagcgg gatcaccgcc ggaacaatcg ccggcaggct ttttacgtcc gggttatagc
    86281 gatagcgcgt ctcaatattc atcagcccgc tttggctggc gggtgtggac tgtcggctcg
    86341 ccacatcctg tagccagctc tggtgcatgg cctgcacgta gccttttacc gtttcggcgc
    86401 ggctcggcat cgcaccgtcg atccagacac cgagttccac gggcgtacca cgcgcgatat
    86461 cgcgcccgaa attgggaggg atctcaattg ccaccgtgat atcgcccgca cgcatccgac
    86521 gatcaagctc gtcataactg gtgagcggtg gctgttcgat aaagtaacgg gaaccggaga
    86581 gattgagcgt ccacgcctgg ctactgacgg tttgatcgcg gtcaagcacc gcaaagcgca
    86641 ggttttccac atccatactg atgccgtaac ccattatcag catcaggatc accgttccca
    86701 tcagcgccag cgtcgaacgt accggatcgc gtcgcagttc cagcgcttcg cggcggctgt
    86761 agctaaacag acggcgcagg ctgaatccct gacgtggcgc gtgggtagtg tcatgtacca
    86821 caggcggcgc ttcggcttcg ttgctctgcc ctgccgcttc ctgcaaataa gcgataaatg
    86881 cctcttcgag actggcggct ccgcgtttct caaccaactc ctgcggtgta ccgctggcga
    86941 gcacttttcc ggcgtgcatc agtgagatac ggtcacaacg ttccgcttcg ttcataaagt
    87001 gggtggagat gaagatagtc actttgtcct ggcgcgagag atccaccatc aactgccaga
    87061 acatatccct cgccaccgga tcgacgccag aagtcggctc atcgaggatt agcatctccg
    87121 ggcgatgaat caccgccacc gccagcgata gccgctggcg aatgccgagc ggcaatgact
    87181 ccggcaatac atcttcgacg tcgttgagct taaaacgctc gctcatttca gccactcgcg
    87241 cgggaatttc cgcttccggg atatgaaaca gacgagcatg taactccagg ttttgccgca
    87301 cggtgagttc gttatagagc gaaaacgcct gcgacatata gcccacacga cggcgggtat
    87361 cgatatcttt tggatcaacc ggctgcccga acagccacgc ctcaccttcg ctggcgggca
    87421 gcaatccggt aagcattttc atggtggtgg atttaccgca gccattcgaa ccaagaaaac
    87481 caaaaatctc cccgcgtgga atgcggaaat tgacgtgatc aacggcaacg aaggaaccaa
    87541 aacgcatggt cagatcgcgc gcttcgatgg caatctctgc gttttcaggt tgatacggtg
    87601 ggatcactac cgcctgatgc gcctggcgtt gtgcttgcgg taacagattt ataaatgctt
    87661 cttccagcgt ggcgctttgc gtttgctgcc gcaactcttc ggcgctaccg gttgccagca
    87721 cttctccggc attcatcgct accagccagt cgaagcgttc ggcctcttcc atgtaggcgg
    87781 tggcaaccag cacgctcata ttgctctgcc gctggcgaat actgtcgatc agatcccaga
    87841 actgggcgcg agagagcggg tcaacccccg ttgttggctc atcaagaatc aacaattccg
    87901 ggtcgtggat taacgcacag cacagcccaa gtttttgctt catcccgccg gagagtttcc
    87961 ctgccggacg atcgcgaaac ggtgctaatc cggtgctggt cagcagctca ttaattcgca
    88021 cttcccgctc cgctttgtcg tgaccgaaca ggcgggcgaa aaaatcgacg ttttcataca
    88081 ccgacaaggt gtggtagaga tttttgccca gcccctgcgg catccaggcg atgcgcgggc
    88141 agacgtcgcg gcgatgcttc gggtcgcgca tatcgccgcc cagcaccatc acgccgccct
    88201 gctcaatgac gcgagcaccg gaaatcaacg acaacaagct cgatttaccg acaccatccg
    88261 gtccaatcag cccgaccatg caacgggccg gaatatcgag tgtgatattg ttcagcgcaa
    88321 cggtttttcc ataatgctgg ctcacgcccg ccagttgcgc gacaggcggg acgggaacca
    88381 gttccagatg catcattgcg gcaacctcac caccaggtcg tcaggccacg gaagttcttc
    88441 attcacccgc acccacgcta cgcccggcaa tccggttttg acatattcca gatgctgctg
    88501 gagtaattcc ggtgggatac gcgctttgac acggaacatc aatttcagcc gctcatcgct
    88561 ggtttcgaca gtttttggcg tgaactgggc gacgctggcg acaaaactga tggtcgcggg
    88621 aatacgcaga tctggtgcag catcaagaat caaacgtgct tcaccgccca gtttcagcgt
    88681 gcccgcctgt tcggttggca gaaagaaggt catatagacg tcgctgagat cgaccatatt
    88741 cagcacccga ccgcctgctg ccagcacttc gcctggctcg gcaacccgat actgcacgcg
    88801 tccgtcacgc ggggctttca gttcgctgtc atcgatatct gcggcaatgc gccgttcagt
    88861 ggcttgtgcc gcttcgacgc gagtttgcgc ctgaatgata ttggtgcgcg ccgcctctat
    88921 agcggcttta gaagcagaaa cctgagcttt tgccgattcc agcgcagctc gggcgctctc
    88981 agcggcggcg cggtcgtcat ccaactgttg cgcagaaatt gccccccgtt gtgccagtga
    89041 gcgggaacgc gtatgacgtt ttgctacgga gtccagttct gcctggcgtt gattaaccag
    89101 cgactgtgcg gcacgagttt cgctttggcg ttgctccagc aaagcctgcg cggcggcaac
    89161 ggcactttgt gcttctttga tttgcgcgat ggcttccagt cgctgttcct gcaacacgcg
    89221 agtatccatc ttcgccagca cttcaccttc tcgaacaaac tggccttctt tcaccagaat
    89281 ggtgtcgata cgcccggcaa ttttgctggc gatatccact tccgtcgctt caatgcgccc
    89341 attgctgaca gcaaagcctt ccggcacacc cgccgggcgc aacagccacc acgccacgac
    89401 ggcagccacc gccagtgccc cgacacccca ccacgccaga tggcgcttac tcttatccat
    89461 aatcgacccg ccataatccc tgtaagcaat cgacacgtgt tacaactgcc accgaacgac
    89521 gttcagcgga ttcttccatc cgggataaga aggaaatggc aaatacgacc agcgcacaga
    89581 gcattgagcg ccaatgggga aggcttagtg aaaacgggta gtaatgttgt catcatcagc
    89641 attcctggcc gtaaatgaaa cacgcatact cataagtgaa atcaattaaa tactaacagt
    89701 tatattcatg ttgtctattt gattgccatc aacttgtgat acatcgcgat gttcttccgt
    89761 tcgctggtgt tttttccaaa aatattctgt acaggttcac agattctgtt gcacaggttt
    89821 ccgtaacagg gaaacctgat gactatcaca tagcgttatt atcgtctgat gtgcgaataa
    89881 ataccgaata taaaataatt tattctgaaa agaatgatag atgataaagg ctcgtgggtt
    89941 taataaaata gtccctaaaa gattcattta aaacaacaaa ataagataaa gatattaatt
    90001 c
//
LOCUS       DAJOTB010000003        86963 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJOTB010000003 DAJOTB010000000
VERSION     DAJOTB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.003"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            83..217
                     /gene="pyrL"
                     /locus_tag="OHN28_000161"
     CDS             83..217
                     /gene="pyrL"
                     /locus_tag="OHN28_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCQ2295612.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            221..1156
                     /gene="pyrB"
                     /locus_tag="OHN28_000162"
     CDS             221..1156
                     /gene="pyrB"
                     /locus_tag="OHN28_000162"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCQ2295613.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            1169..1630
                     /gene="pyrI"
                     /locus_tag="OHN28_000163"
     CDS             1169..1630
                     /gene="pyrI"
                     /locus_tag="OHN28_000163"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCQ2295614.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            1703..2089
                     /gene="ridA"
                     /locus_tag="OHN28_000164"
     CDS             1703..2089
                     /gene="ridA"
                     /locus_tag="OHN28_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCQ2295615.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(2295..4991)
                     /gene="mgtA"
                     /locus_tag="OHN28_000165"
     CDS             complement(2295..4991)
                     /gene="mgtA"
                     /locus_tag="OHN28_000165"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCQ2295616.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(5132..5185)
                     /gene="mgtL"
                     /locus_tag="OHN28_000166"
     CDS             complement(5132..5185)
                     /gene="mgtL"
                     /locus_tag="OHN28_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCQ2295617.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            5370..6317
                     /gene="treR"
                     /locus_tag="OHN28_000167"
     CDS             5370..6317
                     /gene="treR"
                     /locus_tag="OHN28_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCQ2295618.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            6436..7857
                     /gene="treB"
                     /locus_tag="OHN28_000168"
     CDS             6436..7857
                     /gene="treB"
                     /locus_tag="OHN28_000168"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCQ2295619.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLSQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            7907..9562
                     /gene="treC"
                     /locus_tag="OHN28_000169"
     CDS             7907..9562
                     /gene="treC"
                     /locus_tag="OHN28_000169"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCQ2295620.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAVWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWHWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPHGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPDGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            9956..12094
                     /gene="nrdD"
                     /locus_tag="OHN28_000170"
     CDS             9956..12094
                     /gene="nrdD"
                     /locus_tag="OHN28_000170"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCQ2295621.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            12252..12716
                     /gene="nrdG"
                     /locus_tag="OHN28_000171"
     CDS             12252..12716
                     /gene="nrdG"
                     /locus_tag="OHN28_000171"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCQ2295622.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     MWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(12761..13147)
                     /gene="cybC"
                     /locus_tag="OHN28_000172"
     CDS             complement(12761..13147)
                     /gene="cybC"
                     /locus_tag="OHN28_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCQ2295623.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(13202..14554)
                     /gene="pmbA"
                     /locus_tag="OHN28_000173"
     CDS             complement(13202..14554)
                     /gene="pmbA"
                     /locus_tag="OHN28_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCQ2295624.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            14648..15199
                     /locus_tag="OHN28_000174"
     CDS             14648..15199
                     /locus_tag="OHN28_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCQ2295625.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(15357..16730)
                     /gene="mpl"
                     /locus_tag="OHN28_000175"
     CDS             complement(15357..16730)
                     /gene="mpl"
                     /locus_tag="OHN28_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCQ2295626.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            16906..17904
                     /gene="fbp"
                     /locus_tag="OHN28_000176"
     CDS             16906..17904
                     /gene="fbp"
                     /locus_tag="OHN28_000176"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCQ2295627.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(17937..18932)
                     /gene="yjfF"
                     /locus_tag="OHN28_000177"
     CDS             complement(17937..18932)
                     /gene="yjfF"
                     /locus_tag="OHN28_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCQ2295628.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIVQR"
     gene            complement(18919..19941)
                     /locus_tag="OHN28_000178"
     CDS             complement(18919..19941)
                     /locus_tag="OHN28_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2295629.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(19955..21457)
                     /locus_tag="OHN28_000179"
     CDS             complement(19955..21457)
                     /locus_tag="OHN28_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2295630.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVNFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTPRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(21670..22626)
                     /gene="ytfQ"
                     /locus_tag="OHN28_000180"
     CDS             complement(21670..22626)
                     /gene="ytfQ"
                     /locus_tag="OHN28_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCQ2295631.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22936..23466
                     /gene="ppa"
                     /locus_tag="OHN28_000181"
     CDS             22936..23466
                     /gene="ppa"
                     /locus_tag="OHN28_000181"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCQ2295632.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(23844..24185)
                     /locus_tag="OHN28_000182"
     CDS             complement(23844..24185)
                     /locus_tag="OHN28_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2295633.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(24188..27967)
                     /gene="tamB"
                     /locus_tag="OHN28_000183"
     CDS             complement(24188..27967)
                     /gene="tamB"
                     /locus_tag="OHN28_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCQ2295634.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLIGLLIALPKVAEAAQEEVVEPKIENPQPEDKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPISGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLSGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(27964..29697)
                     /gene="tamA"
                     /locus_tag="OHN28_000184"
     CDS             complement(27964..29697)
                     /gene="tamA"
                     /locus_tag="OHN28_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCQ2295635.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            29904..30542
                     /gene="msrA"
                     /locus_tag="OHN28_000185"
     CDS             29904..30542
                     /gene="msrA"
                     /locus_tag="OHN28_000185"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCQ2295636.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            30865..32208
                     /locus_tag="OHN28_000186"
     CDS             30865..32208
                     /locus_tag="OHN28_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2295637.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKMPDAKDKEESVA"
     gene            complement(32269..32475)
                     /locus_tag="OHN28_000187"
     CDS             complement(32269..32475)
                     /locus_tag="OHN28_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCQ2295638.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            32800..33357
                     /locus_tag="OHN28_000188"
     CDS             32800..33357
                     /locus_tag="OHN28_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2295639.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(33347..34087)
                     /gene="cysQ"
                     /locus_tag="OHN28_000189"
     CDS             complement(33347..34087)
                     /gene="cysQ"
                     /locus_tag="OHN28_000189"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCQ2295640.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            34277..36220
                     /gene="cpdB"
                     /locus_tag="OHN28_000190"
     CDS             34277..36220
                     /gene="cpdB"
                     /locus_tag="OHN28_000190"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCQ2295641.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(36349..36729)
                     /locus_tag="OHN28_000191"
     CDS             complement(36349..36729)
                     /locus_tag="OHN28_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCQ2295642.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLTQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            36818..37678
                     /gene="qorB"
                     /locus_tag="OHN28_000192"
     CDS             36818..37678
                     /gene="qorB"
                     /locus_tag="OHN28_000192"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2295643.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHANSSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDRAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            37787..38752
                     /locus_tag="OHN28_000193"
     CDS             37787..38752
                     /locus_tag="OHN28_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2295644.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            38860..39522
                     /gene="ytfE"
                     /locus_tag="OHN28_000194"
     CDS             38860..39522
                     /gene="ytfE"
                     /locus_tag="OHN28_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCQ2295645.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKNWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(39567..40979)
                     /gene="cycA"
                     /locus_tag="OHN28_000195"
                     /gene_synonym="dagA"
     CDS             complement(39567..40979)
                     /gene="cycA"
                     /locus_tag="OHN28_000195"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCQ2295646.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKQAAELRK"
     gene            complement(41288..41908)
                     /gene="fklB"
                     /locus_tag="OHN28_000196"
     CDS             complement(41288..41908)
                     /gene="fklB"
                     /locus_tag="OHN28_000196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2295647.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            42126..42764
                     /gene="ytfB"
                     /locus_tag="OHN28_000197"
     CDS             42126..42764
                     /gene="ytfB"
                     /locus_tag="OHN28_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCQ2295648.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(42748..43395)
                     /locus_tag="OHN28_000198"
     CDS             complement(42748..43395)
                     /locus_tag="OHN28_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCQ2295649.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            43608..44399
                     /locus_tag="OHN28_000199"
     CDS             43608..44399
                     /locus_tag="OHN28_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCQ2295650.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            44409..45251
                     /locus_tag="OHN28_000200"
     CDS             44409..45251
                     /locus_tag="OHN28_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2295651.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            45261..46037
                     /locus_tag="OHN28_000201"
     CDS             45261..46037
                     /locus_tag="OHN28_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCQ2295652.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            46047..47588
                     /locus_tag="OHN28_000202"
     CDS             46047..47588
                     /locus_tag="OHN28_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2295653.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            47585..49657
                     /locus_tag="OHN28_000203"
     CDS             47585..49657
                     /locus_tag="OHN28_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCQ2295654.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            49750..51027
                     /locus_tag="OHN28_000204"
     CDS             49750..51027
                     /locus_tag="OHN28_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2295655.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATS"
     gene            complement(51170..52437)
                     /locus_tag="OHN28_000205"
                     /pseudo
     CDS             complement(51170..52437)
                     /locus_tag="OHN28_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            complement(52440..52931)
                     /locus_tag="OHN28_000206"
     CDS             complement(52440..52931)
                     /locus_tag="OHN28_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2295656.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            complement(53333..53782)
                     /gene="rplI"
                     /locus_tag="OHN28_000207"
     CDS             complement(53333..53782)
                     /gene="rplI"
                     /locus_tag="OHN28_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCQ2295657.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(53824..54051)
                     /gene="rpsR"
                     /locus_tag="OHN28_000208"
     CDS             complement(53824..54051)
                     /gene="rpsR"
                     /locus_tag="OHN28_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCQ2295658.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(54056..54370)
                     /gene="priB"
                     /locus_tag="OHN28_000209"
     CDS             complement(54056..54370)
                     /gene="priB"
                     /locus_tag="OHN28_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCQ2295659.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(54377..54772)
                     /gene="rpsF"
                     /locus_tag="OHN28_000210"
     CDS             complement(54377..54772)
                     /gene="rpsF"
                     /locus_tag="OHN28_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCQ2295660.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            55100..55375
                     /locus_tag="OHN28_000211"
     CDS             55100..55375
                     /locus_tag="OHN28_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2295661.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(55504..56190)
                     /gene="ulaF"
                     /locus_tag="OHN28_000212"
     CDS             complement(55504..56190)
                     /gene="ulaF"
                     /locus_tag="OHN28_000212"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCQ2295662.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFVWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(56190..57044)
                     /gene="ulaE"
                     /locus_tag="OHN28_000213"
     CDS             complement(56190..57044)
                     /gene="ulaE"
                     /locus_tag="OHN28_000213"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCQ2295663.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            complement(57054..57704)
                     /gene="ulaD"
                     /locus_tag="OHN28_000214"
     CDS             complement(57054..57704)
                     /gene="ulaD"
                     /locus_tag="OHN28_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2295664.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(57718..58182)
                     /gene="ulaC"
                     /locus_tag="OHN28_000215"
     CDS             complement(57718..58182)
                     /gene="ulaC"
                     /locus_tag="OHN28_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCQ2295665.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(58192..58497)
                     /gene="ulaB"
                     /locus_tag="OHN28_000216"
     CDS             complement(58192..58497)
                     /gene="ulaB"
                     /locus_tag="OHN28_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCQ2295666.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(58513..59910)
                     /gene="ulaA"
                     /locus_tag="OHN28_000217"
     CDS             complement(58513..59910)
                     /gene="ulaA"
                     /locus_tag="OHN28_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCQ2295667.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFASRAL
                     RAEEDAEKQLAEQSA"
     gene            60265..61329
                     /gene="ulaG"
                     /locus_tag="OHN28_000218"
     CDS             60265..61329
                     /gene="ulaG"
                     /locus_tag="OHN28_000218"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCQ2295668.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            61437..62192
                     /gene="ulaR"
                     /locus_tag="OHN28_000219"
     CDS             61437..62192
                     /gene="ulaR"
                     /locus_tag="OHN28_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCQ2295669.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(62189..62938)
                     /gene="yjfP"
                     /locus_tag="OHN28_000220"
     CDS             complement(62189..62938)
                     /gene="yjfP"
                     /locus_tag="OHN28_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2295670.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            63120..63449
                     /gene="bsmA"
                     /locus_tag="OHN28_000221"
     CDS             63120..63449
                     /gene="bsmA"
                     /locus_tag="OHN28_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCQ2295671.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            63598..63873
                     /gene="yjfN"
                     /locus_tag="OHN28_000222"
     CDS             63598..63873
                     /gene="yjfN"
                     /locus_tag="OHN28_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCQ2295672.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(63990..65615)
                     /locus_tag="OHN28_000223"
     CDS             complement(63990..65615)
                     /locus_tag="OHN28_000223"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCQ2295673.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(65699..66862)
                     /locus_tag="OHN28_000224"
     CDS             complement(65699..66862)
                     /locus_tag="OHN28_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2295674.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(66865..67503)
                     /locus_tag="OHN28_000225"
     CDS             complement(66865..67503)
                     /locus_tag="OHN28_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCQ2295675.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(67513..67911)
                     /locus_tag="OHN28_000226"
     CDS             complement(67513..67911)
                     /locus_tag="OHN28_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCQ2295676.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(67929..68588)
                     /locus_tag="OHN28_000227"
     CDS             complement(67929..68588)
                     /locus_tag="OHN28_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCQ2295677.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(68638..69336)
                     /locus_tag="OHN28_000228"
     CDS             complement(68638..69336)
                     /locus_tag="OHN28_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCQ2295678.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(69355..69756)
                     /locus_tag="OHN28_000229"
     CDS             complement(69355..69756)
                     /locus_tag="OHN28_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCQ2295679.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNNESALIIKLNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(69883..70614)
                     /gene="rlmB"
                     /locus_tag="OHN28_000230"
     CDS             complement(69883..70614)
                     /gene="rlmB"
                     /locus_tag="OHN28_000230"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCQ2295680.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(70705..73146)
                     /gene="rnr"
                     /locus_tag="OHN28_000231"
     CDS             complement(70705..73146)
                     /gene="rnr"
                     /locus_tag="OHN28_000231"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCQ2295681.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNVERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(73185..73610)
                     /gene="nsrR"
                     /locus_tag="OHN28_000232"
     CDS             complement(73185..73610)
                     /gene="nsrR"
                     /locus_tag="OHN28_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCQ2295682.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(73815..75113)
                     /gene="purA"
                     /locus_tag="OHN28_000233"
     CDS             complement(73815..75113)
                     /gene="purA"
                     /locus_tag="OHN28_000233"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCQ2295683.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(75217..75414)
                     /locus_tag="OHN28_000234"
     CDS             complement(75217..75414)
                     /locus_tag="OHN28_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCQ2295684.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(75496..76500)
                     /gene="hflC"
                     /locus_tag="OHN28_000235"
     CDS             complement(75496..76500)
                     /gene="hflC"
                     /locus_tag="OHN28_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCQ2295685.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(76503..77762)
                     /gene="hflK"
                     /locus_tag="OHN28_000236"
     CDS             complement(76503..77762)
                     /gene="hflK"
                     /locus_tag="OHN28_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCQ2295686.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(77848..79128)
                     /gene="hflX"
                     /locus_tag="OHN28_000237"
     CDS             complement(77848..79128)
                     /gene="hflX"
                     /locus_tag="OHN28_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCQ2295687.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(79204..79512)
                     /gene="hfq"
                     /locus_tag="OHN28_000238"
     CDS             complement(79204..79512)
                     /gene="hfq"
                     /locus_tag="OHN28_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCQ2295688.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(79598..80548)
                     /gene="miaA"
                     /locus_tag="OHN28_000239"
     CDS             complement(79598..80548)
                     /gene="miaA"
                     /locus_tag="OHN28_000239"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCQ2295689.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(80541..82388)
                     /gene="mutL"
                     /locus_tag="OHN28_000240"
     CDS             complement(80541..82388)
                     /gene="mutL"
                     /locus_tag="OHN28_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCQ2295690.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(82398..83729)
                     /gene="amiB"
                     /locus_tag="OHN28_000241"
     CDS             complement(82398..83729)
                     /gene="amiB"
                     /locus_tag="OHN28_000241"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCQ2295691.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(83748..84209)
                     /gene="tsaE"
                     /locus_tag="OHN28_000242"
     CDS             complement(83748..84209)
                     /gene="tsaE"
                     /locus_tag="OHN28_000242"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCQ2295692.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(84181..85728)
                     /gene="nnr"
                     /locus_tag="OHN28_000243"
     CDS             complement(84181..85728)
                     /gene="nnr"
                     /locus_tag="OHN28_000243"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCQ2295693.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACVGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            85727..86866
                     /gene="queG"
                     /locus_tag="OHN28_000244"
     CDS             85727..86866
                     /gene="queG"
                     /locus_tag="OHN28_000244"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCQ2295694.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(86849..86902)
                     /locus_tag="OHN28_000245"
     CDS             complement(86849..86902)
                     /locus_tag="OHN28_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295695.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT        21148 a        23383 c        21602 g        20830 t
ORIGIN      
        1 ttgccttgct ttataactgc atcatcttct tgatcctctc tcagagctga gtataatgcc
       61 ggacaatttg ccgggaggat gtatggttca gtgtgttcga cattttgtct taccgcgtct
      121 gaaaaaagac gctggcctgc cgtttttctt cccgttgatc acccattccc agcccctcaa
      181 tcgaggggct tttttttgcc caggcgtcag gagataaaag atggctaatc cgctatatca
      241 gaaacatatc atttccataa acgaccttag tcgcgatgac cttaatctgg tgctggcgac
      301 agcggcgaaa ctgaaagcga acccgcaacc agaactgttg aagcacaaag ttattgccag
      361 ctgtttcttc gaagcctcta cccgcacccg cctctctttc gaaacttcca tgcaccgcct
      421 gggtgccagc gtggtgggct tctccgacag cgccaataca tcactgggta aaaagggcga
      481 aacgctggcc gataccattt cggttatcag cacttacgtc gatgcgatag tgatgcgtca
      541 tccgcaggaa ggtgcagcgc gcctggccac cgagttttcc ggcaatgtac cggtactgaa
      601 tgccggtgat ggctccaacc aacatccgac gcaaaccttg ctggacttat tcaccattca
      661 ggaaacccag gggcgtctgg acaatctcca cgtcgcaatg gttggtgacc tgaaatatgg
      721 ccgcaccgtt cactccctga cccaggcgct agcgaagttc gacggcaatc gtttttactt
      781 tatcgcgccg gatgcgctgg caatgccgca atacattctg gatatgctcg atgaaaaagg
      841 gatcgcctgg agtctgcaca gctctattga agaagtgatg gcggaagtag acatcctgta
      901 catgacccgc gtgcaaaaag agcgtctgga cccgtccgag tacgccaacg tgaaagcaca
      961 gtttgttctt cgcgccagcg atctccacaa cgccaaagcc aatatgaaag tgctgcatcc
     1021 gctgccgcgt gttgatgaga ttgcgacgga tgtggataaa acgcctcacg cctggtactt
     1081 ccagcaagca ggcaacggga ttttcgctcg ccaggcgtta ctggcactgg ttctgaatcg
     1141 cgatctggta ctgtaagggg aaatagagat gacacacgat aataaattgc aggttgaagc
     1201 tattaaacgc ggcacggtaa ttgaccatat ccccgcccag atcggtttta agctgttgag
     1261 tctgttcaag ttgaccgaaa cagatcagcg catcaccatt ggtctgaatt tgccttctgg
     1321 cgagatgggg cgcaaggatc tgatcaaaat cgaaaatacc tttttaagtg aggatcaggt
     1381 tgatcagctg gcgttatatg caccgcaagc cacggttaac cgtatcgaca actatgaagt
     1441 ggttggcaaa tcgcgcccca gtctgccaga gcgcatcgac aatgtgctgg tctgcccgaa
     1501 cagcaactgt atcagccatg ccgaaccggt ttcatccagc tttgccgtgc gaaaacgcgc
     1561 caatgatatc gcgctcaaat gcaaatactg tgaaaaagag ttttcccata atgtggtgct
     1621 ggccaattaa ttgcggttgg taataaaagt ctggctccct ataatgagcc agacttttta
     1681 ccgctgtaat aaaggagaaa tcatgagcaa aactatcgcg acggaaaatg caccggcagc
     1741 tatcggtcct tacgtgcagg gcgttgatct gggcaatatg attatcactt ccggtcagat
     1801 cccggtaaac ccgaaaacgg gcgaagtccc ggcagatgtc gctgcacagg cgcgtcagtc
     1861 gctggataac gtaaaagcga tcgtcgaagc cgctggcctg aaagtgggcg atatcgttaa
     1921 aaccaccgtg tttgtgaaag atctgaacga cttcgcaacc gttaatgcta cctacgaagc
     1981 cttcttcacc gaacacaacg ccaccttccc ggcacgttct tgcgttgaag ttgcccgtct
     2041 gccgaaagac gtgaagattg agatcgaagc gatcgctgtt cgtcgctaat cttgatggaa
     2101 atccgggcta tcatgcccgg attaagtcta atgacaaacg caaaattgcc tgatgcgcta
     2161 cgcttatcag gcctacgtga ttcctgcaat ttattgaatt tgtaggcctg ataaggcatt
     2221 tacgccgcat ccggcatgaa caaagctcac tttgtctgca atctgattcc gggccaccat
     2281 gcccagttta ttctttattg ccagccgtaa cgacggctat agaacccttt caccaactgg
     2341 gttaacgtca tataccctgc cagaatcgca accagccacg ggaaatagct taacggcagc
     2401 gcctgcagtt gcagataact ggccagcggt gaaaaaggca atgcgatccc gacaatcatc
     2461 acgatcacgg tcatgatcat taacggccac gatgcacagc tctgaataaa cggcacacgg
     2521 cgggtgcgga tcatatgcac aatcagcgtt tgcgacagta agcccaccac aaaccagccc
     2581 gactggaaca gcgtttgcgt ttccggcgtg ttggcatgga atacccacca catcaggcaa
     2641 aacgtcagaa tatcgaaaat cgagctgatc ggtccgaaga agaccataaa gcgccccaga
     2701 tccgccggat tccaacgctg cggcttttga atttgctcgt cgtcgacgtt atcaaatggg
     2761 atcgccacct gtgacacatc gtacagcaga ttttgaataa gcaagtgtag cggcaacatc
     2821 ggcaggaagg gcaagaaagc actcgccacc agcacgctga acacattacc gaagttagag
     2881 ctcgccgtca ttttgatgta tttcagcatg ttggcgaaag tgcgacgtcc ctcaataacc
     2941 ccctcttcca gcaccatcag gcttttttcc agcaggatga tatcagccgc ttcacgggca
     3001 atatctaccg cgccgtccac agaaatgccg atatccgcag cgcgtaaggc gggcgcatca
     3061 ttaataccat cgcccataaa accaaccaca tgcccttcgc gcttcagcaa cgttacgatg
     3121 cgttctttat gcatcggcgt cagacgagca aacagcgtgg tgcgctgtgc gagatttgcc
     3181 agttcgtcgt cagatagcgt ttcaatatca ctaccaatga ccacctctcc cgcgtccagc
     3241 cccacttcat ggcacacttt cgctgcaacc aactcactgt cgccagtaag gatttttacg
     3301 gtaattccac tcgcttttaa tgccttcagt gccggagctg ttgtctcttt tggcggatca
     3361 aggaaagcaa tatatccttc gaggatcagg tcggattcat ccgcccgctg gtaatctcct
     3421 tcacgcgctg gcaggtattt cgtcgccacc gcaaccacgc gcagcccctg acgattcagc
     3481 gtatcagtaa cccgcttaat cttacgcagc atggtgtcat cgagcggtac aatctcgcca
     3541 ttgtgacgca cttgcgaaca cacattgagg atttcctgca atgcgccttt gcaaaccaac
     3601 tgatgatgtt cggtattttc tgccacaacc accgacatcc gacgacgctc gaaatcaaac
     3661 ggaatctcat caattttttg ccaacggctg gccagcgagc gcgctgactc ttcatccgta
     3721 ccttcgagca ccgctgtatc aagcaggttt ttaagcccgg tctggtaatg gctgttcaac
     3781 cacgcgctat gcagcacgcg ttcgctagtt ttaccggaga tatcggtatg attctccagc
     3841 acaattttat cctgtgtcag ggtgccggtt ttatcagtgc acagaatatc cattgcgcca
     3901 aagttctgaa tggcatccag atgtttgacg atcactttct gtttcgacaa cttgactgcg
     3961 ccacgcgcca gcgtcgaggt aacaatcatc ggcaacattt ctggcgttaa gcctaccgcg
     4021 accgaaagcg caaacagtgc agcttcccac cagtcgcctt tagtgtaacc attgataagc
     4081 aacaccaccg gagccatcac cagcataaag cgaatcagca gcatactgac gcggctaatc
     4141 ccttgctgaa aggcattcgg ctcgctttcc tgctcactaa cacgacccgc cagttgacca
     4201 aaccaggtat tggcacctgt ggcaatcacc attgcttgtg ccgtgccgct caccacggtg
     4261 gtgcccataa aacacagggt gtcgcactcc agcggattgc tgtgctccgg ctggcgagtg
     4321 gtagcggctt tttccacggg cagagactca ccggttaacg acgcctgagc gacaaacaga
     4381 tcccgcgcct gcaagatacg taaatctgcc gggatcatat cccccgccgc cagttttata
     4441 atatcgccgg gcaccagctg gtctatcggg atctccagcc agccattttc gcctttgtcg
     4501 ttaattacgc gcagcaccgt cgcagtattg ctgaccatcg ctttcagggc atctgccgct
     4561 ttggtggaac gagcttcctg aataaagttc agcaacgtag aaatagcgac cattagcgcg
     4621 ataacacctg cggcaaataa atcttccgtg gcgtaagaaa tagcaccaag aatggtgagc
     4681 aaaatattaa aggggttgcg atagcagacc cataaatgta cccaccacgg cgacggttgt
     4741 tgtgcgggta atttattttc accatgttgt tcgcgggcag attccacttc cgccagattt
     4801 aacccttccg gatgggtgtc gaacgttttc cacaattctt cttcgggcat caccgccatt
     4861 ttcaggcaat gcgcacttaa agaaggcggg accgccgcat tcactgtctg ctgcgcgcca
     4921 ggcaatggat cacgatgaac cagacgggaa ggtaaatggc gaatgagccg ggtaaaaatt
     4981 tctttaaaca taaggagtcc ctccgcactg tctgaaacag tacagaaatg tcttcaggcg
     5041 tggcgaagcc ttacctgaag gtaataaagc tgagcaggga cgttaaaacg tcacggcctt
     5101 acgcatccgg taacgccgtg aaaagacggg cttaccggaa aagttttaat ctccgtcgag
     5161 ggagaggcgt gggatcaggt tccatataac ctccggtaag tgaaaaaagc gccgcaaata
     5221 ataaggagaa tccaataata ttcgtagcat ttatggcgat ataatacctg ctggcaacta
     5281 aaccaacgat aaaccagact ttacccattg ctgaatgcac aggtaaagtt aggctcaaat
     5341 aattaaacaa cacgttatgg gacaacagga tgcaaaatcg gctgaccatc aaagacatcg
     5401 cgcgcttaag cggcgtgggg aaatctacgg tttcccgggt gctgaataac gaaagcggcg
     5461 tgagccagcg cacgcgcgag cgtgttgaag cagtgatgaa tcagcatgga ttttcccctt
     5521 cccgctctgc gcgcgctatg cgtgggcaaa gcgataaagt ggtcgccatc attgttaccc
     5581 gtctggattc gttgtcagaa aatctcgccg ttcaaaccat gctgccagcg ttctatgaac
     5641 aaggttacga tccaatcatg atggaaagtc agttttcccc acaattagtt gccgaacatt
     5701 tgggggtgct gaaacggcgt aatatcgacg gcgtagtgct gttcggtttt accggcataa
     5761 cagaagaaat gttagcccac tggcagtcat cgctggttct gctggcgcgt gacgcaaaag
     5821 gctttgcttc ggtctgttat gacgacgaag gggcaatcaa gatcctgatg caacggctgt
     5881 atgaccaggg acatcgtaat atcagctatc tcggcgtgcc gcacagtgac gtgacaaccg
     5941 gtaagcgacg tcacgaagcc tacctggcgt tctgcaaaac gcataaattg catcccgttg
     6001 ccgctctgcc agggcttgct atgaagcaag gctatgagaa cgtagcaaaa gtgattacgc
     6061 ctgaaactac cgcgttactg tgcgcaaccg acacgctggc acttggcgca agtaaatacc
     6121 tgcaagagca acgcatcgac accttgcaac tggcgagcgt cggtaataca ccgttaatga
     6181 aattcctcca tccggagatc gtcaccgtag atcccggtta tgccgaagct ggacgccagg
     6241 cggcctgcca gttgatcgcg caggtaaccg ggcgcagcga accgcaacaa atcatcatcc
     6301 ccgccaccct gtcctgatcg tttcctgaac gataaattgt gatcttcgct gcgtttcggg
     6361 aacgttcccg tttttaaatt tttccgcgca atatattctg cagccaacca aaaatgtcat
     6421 ctgccatggg gctttatgat gagcaaaata aaccaaacgg atatcgatcg gttgattgaa
     6481 ctggtcggcg ggcgcggcaa tattgcgaca gtgagccact gtattactcg cctgcgcttt
     6541 gtcctcagcc aaccggccaa tgccagaccg aaagaaattg agcaactccc catggtgaaa
     6601 ggctgtttca ccaatgccgg gcaatttcag gtggtgattg gcaccaacgt gggtgattac
     6661 tatcaagcac tgattgcgtc aaccggacag gcgcaggttg ataaagagca ggtaaaaaaa
     6721 gccgcccggc agaatatgaa atggcatgag cagttgatct ctcatttcgc ggagatcttc
     6781 ttcccgttgc tgcccgcgtt gattagtggt ggtttgatcc tcggttttcg caatgtgatc
     6841 ggcgatttgc ccatgagcaa cggtcagacg ctggcgcaaa tgtacccttc cctgcaaacg
     6901 atctacgatt ttctgtggtt gatcggtgaa gcgatcttct tctacctgcc ggtcgggatt
     6961 tgctggtcag cggtgaaaaa aatgggcggt acaccgatcc tcggtatcgt gcttggcgta
     7021 acgctggttt ctccacagct gatgaacgct tatctgctcg ggcagcagct gccggaagta
     7081 tgggactttg gcatgttcag catcgccaaa gtgggctatc aggcgcaggt catcccggca
     7141 ctgttagccg ggctggcgct gggcgttatt gaaactcgtc ttaagcgcat cgtgccggat
     7201 tacctctatc tggtggtggt gcccgtctgt tcgctgatcc tcgcggtgtt cctcgcccat
     7261 gcgctgattg gtccgtttgg tcgcatgatt ggcgatggcg ttgcctttgc ggtacgtcac
     7321 ctgatgaccg gcagctttgc tccgattggc gcggcattgt ttggcttcct gtacgccccg
     7381 ctggtgatca ccggcgtaca ccagaccacg cttgctattg atttgcagat gattcaaagc
     7441 atgggtggca cgccagtgtg gccgctgatt gcactgtcga atatcgctca gggctccgcc
     7501 gtgataggta ttatcatttc cagccgcaag cacaatgaac gcgagatctc cgtgcctgcc
     7561 gctatctccg cctggcttgg ggtcactgag cctgctatgt acggcatcaa cctgaaatat
     7621 cgcttcccga tgctgtgcgc gatgattggt tctggtctgg caggattgct atgcggcttg
     7681 aacggcgtta tggcgaatgg catcggcgta ggcggcctgc cgggaattct ctcgattcaa
     7741 ccgagctact ggcaggtgtt tgcgctggca atggctatcg ccatcatcat cccgattgta
     7801 ctcacctcgt ttatctatca gcggaaatac cgcctgggca cgctggacat tgtttaattt
     7861 tcttcggggc gcaattgcgc cccctcgcat ttgcaggaat aacgtaatga ctcatcttcc
     7921 ccactggtgg caaaacggcg ttatctacca gatttatcca aagagttttc aggacaccac
     7981 gggtagcggc accggcgatt tacgtggcgt tatccaacgc ctggactatc tgcataaact
     8041 gggcgttgat gccgtctggc taaccccctt ttatgtctct ccccaggtcg ataacggtta
     8101 cgacgtagcg aattatacgg cgattgatcc cacctatggc acgctggacg attttgacga
     8161 actggtgaca caggcaaaat cgcgtgggat tcgtatcatt ctcgatatgg tgtttaacca
     8221 tacctctacc caacatgcct ggtttcgcga ggcgctgaac aaagaaagcc cttaccgcca
     8281 gttttatatc tggcgcgatg gagaaccaga aacgccaccg aacaactggc gttcaaaatt
     8341 cggtggtagt gcgtggcact ggcatgcgga aagcgaacag tactatttgc atctctttgc
     8401 accagaacag gcggatctca actgggagaa tccagcggta cgcgcagagc tgaaaaaagt
     8461 ctgtgagttc tgggccgatc gtggggtcga cgggttgcgc ctggatgtgg tgaatctgat
     8521 ctccaaagat ccgcgttacc ctgaagacct ggatggcgac gggcgtcgct tctacaccga
     8581 cgggccacga gcacacgaat ttttgcacga gatgaaccgc gatgtgttta cgccacacgg
     8641 gttaatgacc gtaggtgaaa tgtcctccac cagccttgag cattgccagc gatacgcggc
     8701 actgacaggc agtgaattgt cgatgacctt taattttcat cacctgaagg tcgattatcc
     8761 cgatggtgaa aaatggacgc tggctaaacc tgactttgtg gcgttgaaaa cattgttccg
     8821 ccactggcaa caaggaatgc acaacgtggc atggaatgcc ttgttctggt gtaaccacga
     8881 tcagccgcgc attgtttctc gctttggtga tgaaggtgaa taccgcgtgc ctgcggcaaa
     8941 aatgctggcg atggtgctgc atggcatgca gggaacgccg tatatctacc agggcgaaga
     9001 gattggcatg accaacccac atttcacgcg cattactgac tatcgcgacg tggagagcct
     9061 caatatgttt gccgagctgc gcaacgatgg gcgtgatgcc gacgagttat tggcaatcct
     9121 cgccagtaaa tcccgtgaca acagtcgcac acccatgcaa tggaccaacg gcgataatgc
     9181 cgggtttacg gctggcgaac cgtggattgg cctgggcgat aactatcagg aaatcaacgt
     9241 agaagcagcg ctggccgatg agtcctcggt gttttacacc taccaaaagt taatcgcact
     9301 gcgtaagcaa gaagccgtcc tgacatgggg cgattaccag gatctgctgc caaacagccc
     9361 tgtattgtgg tgctatcgcc gtgagtggaa ggggcaaacc ttgctggtca ttgccaacct
     9421 tagccgtgag acccaaccct ggcagcctgg gaaaatgctc ggcaactggc agcttgtgat
     9481 gcataactac gaagaagcct caccacaacc ctgtgccatg actttacggc cttttgaggc
     9541 tgtctggtgg ttacagaagt aaatcttcgc taagccccgg taatgcctgt cattccgggg
     9601 ttttgcctgt cagtatgttg tttttgttga ttttataatc aaaaaattca ttaaaaaatt
     9661 tacatatcgc tgtagcgccc gtcatccgta cgctctgctt tttactttga gctacatcaa
     9721 aaaaagttca aacatccttg atgcaaagca ctatatatag actttaaaat gcgtcccaac
     9781 ccaatatgtt gtattaatcg actataattg ctactacagc tccccacgaa aaaggtgcga
     9841 cgttgtggat aagcggatgg cgattgcgga aagcaccgga aaacgaaacg aaaaaaccgg
     9901 aaaacgcctt tcccaatttc tgtggataac ctgttcttaa aaatatggag cgatcatgac
     9961 accgcatgtg atgaaacgag acggctgcaa agtgccgttt aaatcagagc gcatcaaaga
    10021 agcgattctg cgtgcagcta aagcagcgga agtcgatgat gccgattatt gcgccactgt
    10081 tgctgcggtt gtcagcgagc agatgcaggg ccgcaaccag gtggatatca atgagatcca
    10141 gaccgcagtt gaaaaccagc tgatgtcggg gccatacaaa caactggctc gtgcttacat
    10201 cgagtaccgt cacgatcgcg acattgaacg tgaaaaacgc ggtcgcctga accaggagat
    10261 ccgtggtctg gtcgagcaga ccaactcctc gttactcaac gaaaacgcca acaaagacag
    10321 caaggtgatt ccaacccagc gcgacctgct ggccgggatc gtggctaaac actatgcacg
    10381 ccagcacctg ctgccgcgtg acgtggtgca ggcacatgaa cgtggtgata ttcactatca
    10441 cgatctcgat tactcaccgt tcttcccgat gttcaactgc atgttgatcg acctgaaagg
    10501 catgctgacc cagggcttta aaatggggaa cgccgagatt gaaccgccga aatcgatctc
    10561 tacggcaacc gcggtaactg cgcagattat tgctcaggtc gccagccata tttatggcgg
    10621 caccaccatt aaccgtatcg atgaagtgct ggcaccgttt gtcaccgcca gctacaacaa
    10681 acatcgcaag accgcagaag agtggaacat cccggacgcc gaaggctacg ctaactctcg
    10741 taccatcaaa gagtgctacg atgccttcca gtcgctggag tacgaagtaa acaccctgca
    10801 caccgccaac ggtcaaacac cgtttgtgac ctttggtttt ggtctgggca ccagctggga
    10861 gtcgcgcctg attcaggaat ctatcctgcg taaccgtatc gccggcctcg gtaaaaaccg
    10921 taaaacggcg gtgttcccga aactggtgtt tgcgattcgc gatggcctga accataaaaa
    10981 aggcgatccg aactacgaca tcaaacagct ggcgctggag tgcgcaagca agcgcatgta
    11041 tccggatatc ctgaactacg atcaggtagt gaaagtcacc ggttcgttta aaaccccgat
    11101 gggctgccgc agcttcctcg gcgtgtggga aaatgaaaac ggcgagcaga tccacgatgg
    11161 tcgtaacaac ctcggcgtga tcagcctgaa cctgccgcgt attgctctgg aagcaaaagg
    11221 cgatgaagcc accttctgga agctgctgga tgaacgtctg gtgttggcac gtaaggcact
    11281 gatgacccgt atcgctcgtc tcgaaggcgt gaaagcgcgc gtagccccga tcctctatat
    11341 ggaaggcgct tgtggcgtgc gtctgaatgc tgacgacgac gtttctgaaa tcttcaaaaa
    11401 cggtcgtgcg tctatttcgc tgggttacat cggcatccac gaaaccatta acgcgctgtt
    11461 cggcggcgag catgtttacg acaacgagca gctgcgcgcg aaaggtattg cgattgttga
    11521 gcgtctgcgt caggcggtgg atcagtggaa agaagaaact ggctacggtt tcagcctcta
    11581 cagtacgccg agtgagaacc tgtgcgaccg cttctgccgt ctcgataccg ctgagtttgg
    11641 cgtggtgccg ggcgtgaccg ataaaggtta ctacaccaac agtttccacc tcgatgtgga
    11701 gaagaaggtg aacccgtacg acaagatcga ctttgaagcg ccttacccgc cactggcgaa
    11761 cggtggcttc atttgctatg gcgagtatcc aaacattcag cacaacctga aggcgctgga
    11821 agatgtctgg gattacagct atcagcatgt accgtattac ggcaccaaca caccgatcga
    11881 tgaatgctac gagtgtggct ttaccggcga gtttgagtgc accagcaaag gcttcacttg
    11941 tccgaaatgt ggtaaccatg acacctcccg tgtgtcggtg actcgccgcg tgtgcggata
    12001 tttaggtagc ccggatgcac gtccgtttaa cgctggtaag caggaagaag ttaagcgccg
    12061 cgttaaacat ctgggaaatg ggcagatagg ttaatcttca ctctgtgtta agcaaatgcc
    12121 ggatgcggcg taaacgcctt atccggccta catttggcta cggctgtagg cctgataaga
    12181 cgcgacagcg tcgcatcagg ccttgattac cggatacagc ctacacgcgg actgataagc
    12241 caggtgaaat aatgaactat catcagtact atcctgtcga catcgtcaac ggccccggca
    12301 ctcgctgcac cctgtttgtc tctgggtgtg ttcatgaatg ccccggttgc tataacaaaa
    12361 gcacctggcg ggtaaattcc ggtcagccat ttaccaaagc aatggaagac cagatcatta
    12421 acgatctgaa tgacactcgt atcaaacgcc agggtatttc cctctccggc ggcgatccgc
    12481 tacatccgca aaacgtgccg gatattctga agctggtaca acgcatccgc gccgagtgtc
    12541 cgggtaaaga catctggatg tggacaggct ataaactcga cgaactcaac gctgcgcaaa
    12601 tgcaggttgt tgatttgatt aacgtgctgg tcgacggcaa atttgtgcag gatttaaaag
    12661 acccttccct gatctggcgc ggcagcagca atcaggtggt gcatcatttg cgatgataag
    12721 ttccctgtta cctgagtgca ggcaacaggg aaataaagaa ttaacgatac ttctggtgat
    12781 aggcgttgcg ggtcgttttc agttgctctg cggcagcctg cgcttctttt actttacctt
    12841 catttgccag cttcagcgcg tcgtcaatct gaccgaccag aatgtcgaaa ccgtggcgga
    12901 agtctttcat ttccgggctg tccggtgatt tgccttcaag cttcggcggc gtcgcttttt
    12961 gcgcatccag tgccgcagcg cgcatcttcg ttaacgcgtc tttgacttgc gccgcgttat
    13021 cggctttttc aaccactttt aaattgtcgt tgagggtttc catattgtct tccagatcag
    13081 cggcaaacga cgccgaactg aataccaatg aggagactgc aagaatagct aacaggcttt
    13141 tacgcattgc tcacttcctt ttttattatg aagatttggc gcggcaggtc gcgccagttt
    13201 tttactgccc ggcgattttc atctccggca acagcacaga accacactgt atattactgc
    13261 gtgtttcaat atcgttaccg acggtgacaa tattgcgcca catatctttt aaattaccag
    13321 cgatggtgat ttcgctcacc ggatactgaa tctcgccatt ctctacccag aagcccgctg
    13381 caccacggga ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca
    13441 ccagcccggt gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc
    13501 gccagttgtg aataccgccc gcatgtccgg tgcttttcag ccccagtttc cgcgccgaat
    13561 agctggtcag cagccactga gtcaggatgc catctttgac gatatcgcgg cgctcggtgc
    13621 gcacaccttc gctgtcgaat ggtgtagatg ccagcccttt cagcagatgc ggatgctctt
    13681 caatggtcag ccaatccggc agaatttgtt tacccagcga atccagcaag aaggtcgatt
    13741 tacggtagac cgcaccaccc gctatcgccc ccaccaaatg accaaacaac cctgttgcca
    13801 cttcattggc aaaaatcacc ggcgctttca tggtagaaag tttacgcggt gacaggcgcg
    13861 ataaggtacg gcgagcacag tcggccccaa cccactctgg cgtttgtaga tcgctcatcg
    13921 cacgcccaat ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcgattacac
    13981 agctggagag cgaatgacgc gtagagcagt acccctgcaa catgccgtgg ctgttgccaa
    14041 aaactttgac accgtagtgg ctgttaaagc taccgccttc ggtattggtg atgcgtttgt
    14101 ccgcctgcaa tgccgcctgt tccgcgcgag ccgccagttc aatggcttca tccggagaaa
    14161 cgtccgcagg gtggaacaag tcaagatccg gtgcgtcaaa ggccagcagt tctttgtctg
    14221 ccacgccggc atacggatct ggcgaggtat aacgagcaat atccagcgcc gcctgtacgg
    14281 tgcgggcaat ggcctgcggg cttaaatcgg tagacgatgc gctaccttta cggttctggt
    14341 gataaacggt gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat
    14401 aacgcgtgct tacactaatg ccggtggtct tgctgacggc aacttctgca ccgtccgatt
    14461 tgcctgaggc caactccagc gcagtcgaaa ctgcttcttc cagaatcttg cgctgcgctt
    14521 caacttgaga gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta
    14581 acagagaact gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga
    14641 gcctgagatg actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga
    14701 agacgaagac gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga
    14761 gctaaaacgc cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc
    14821 gttagatgcg gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg
    14881 ccgccgccag ttgcagctca tcggtaagat gctgcgccag cgcgatgttg aaccgatccg
    14941 tcaggcgctg gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact
    15001 ggaaaacctg cgcgatcgtc tgatcgacca gggtgatgac gccatcgctg aagtattgaa
    15061 tctgtggccc gatgccgatc gtcagcaact gcgtactctg atccgtaacg cgaagaaaga
    15121 gaaagaaggg aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgagtt
    15181 agcggagaac gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatacggtgc
    15241 acctgtaggc ctgataagac gcatcaagcg tcgcatcagg cactatgctc aaatgccgga
    15301 tgcggcgtga acgccttatc cggcctacac acactctatc aggctgaggc tgtgaattac
    15361 tgcgcggctt ccgctttctt cgctaaccca tccagcagtt tctgatggat cccaccaaaa
    15421 ccgccgttgc tcatcaccag aatgtgatca cccggctgag cggttttcac caccatatct
    15481 gccagcgtat ccacatcgcc gctccagtgt gcaggctgaa cgcaggcttc tgccacttct
    15541 gccacctgcc acggaatatg tgccggttgc agcaggaaga cttcatcggc acgacctaat
    15601 gaaggtgcca gatcgtcttt gcagatcccc attttcatgg tattagagcg cggttccagt
    15661 acggcaataa tgcgcgccgt accaccaact ttgccacgca gcgccgccag cgttgccaga
    15721 atcgccgtcg ggtgatgggc aaaatcatca tataccgtga cgccattcgc ttcaccacgc
    15781 aactccagac ggcgacgagc gttaataaac gaacccagcg cgttagcggc atctgccggc
    15841 gctacaccaa catggcgagc cgccgcaatc gccatcaggc cattgtgcat attatgttcg
    15901 cctaccagcg accatttcac ttcgcccact ttttcgccat ccagcaagac ttcccattca
    15961 gaagcgtcag tggtcagctt tttcgcctgc cagtgacctt gctcgcccac cagctcctgc
    16021 tcgctccaac agcccatcgc catggtctgt ttcaggttga tgtcgttttc cggccagata
    16081 atacggccct gccccggaac gatacgcacc agatggtgga actgtttctg gatcgctttc
    16141 aggtcgtcaa agatatcggc gtgatcgaac tcaaggttgt tgaggatcag cgtacgcggg
    16201 cagtaatgga caaatttaga gcgtttgtcg aagaaggcgc agtcgtactc atccgcttcg
    16261 ataacaaaga agtcgctttc gcccagacgc gccgaaacct caaagttccc cggcacaccg
    16321 ccaatcacaa atcccggttt gtaaccgcat tgttccagaa tccaggtcgc cattcccgcc
    16381 gtggtggttt tgccgtgtgt accggcaacg gccagcaccc agcggtcgcg cagcacaaaa
    16441 tcgtgcagcc actgcggacc tgacatataa gggatgtttt tttccagtac cgcttccaca
    16501 cacggatttc cacgggtcat ggcgttacca ataatcacca gatccggctg tggatcgagc
    16561 tggctggcat cgtaaccctg aatcagttca atgccttgct tctcaagtaa ggtgctcatc
    16621 ggcggataca cattggcgtc cgaacccgtt acttcatggc ctaactggcg cgccagcatc
    16681 gccagaccgc ccataaacgt gccacaaatt cctaaaatat gaatgcgcat acgtcactat
    16741 ccttttttaa tctggcgctc attttacgca tatgtcgggt aagtgagaaa tgcatttcag
    16801 gataatccgt aatttgctgg cgcgattcac ctgagcgcaa cattgggatt atttgttaag
    16861 attgttgcgg tcgctttact ccataaacat tgcagggaaa gttttatgaa aacgttaggt
    16921 gaatttattg tcgaaaagca gcacgagttt tctcatgcta ccggtgagct cactgctttg
    16981 ctgtcggcaa taaaactggg cgccaagatt atccatcgcg atatcaacaa agcaggactg
    17041 gttgatatcc tgggtgccag cggtgctgag aacgtgcagg gcgaggttca gcagaaactc
    17101 gacctgttcg ctaatgagaa actgaaagcc gcactgaaag cacgcgatat cgttgcgggc
    17161 attgcctctg aagaagaaga tgagattgtc gtctttgaag gctgtgaaca cgcaaaatac
    17221 gtggtgctga tggaccccct ggatggctcg tccaacatcg atgttaacgt ctctgtcggt
    17281 accattttct ccatctaccg ccgcgttacg cctgttggca cgccggtaac ggaagaagat
    17341 ttcctccagc ctggtaacaa acaggttgcg gcaggttacg tggtatacgg ctcctctact
    17401 atgctggttt acaccaccgg atgcggtgtt cacgccttta cttatgatcc ttcgctgggt
    17461 gttttctgcc tgtgccagga acggatgcgc ttcccggaga aaggcaaaac ctactccatc
    17521 aacgaaggaa actacattaa gtttccgaac ggggtgaaga agtacattaa attctgccag
    17581 gaagaagata aatccaccaa ccgcccgtat acctcacgtt atatcggttc actggtcgcg
    17641 gatttccacc gtaacctgct gaaaggcggt atttatctct acccaagcac cgccagccac
    17701 ccggacggta aactacgttt actgtatgag tgcaacccga tggcattcct ggcggaacaa
    17761 gcgggcggta aagcgagcga tggcaaagag cgtattctgg atattatccc ggaaaccctg
    17821 caccagcgcc gttcgttctt tgtcggcaac gaccatatgg ttgaagatgt cgaacgcttt
    17881 atccgtgagt tcccggacgc gtaatctcct gcgcctctcc acacggagag gcgtttttat
    17941 cgctgcacaa tgttgactct tgtcacaggc gaactctgac gattctccca cagcaccgtt
    18001 aatccacgct gtaatgctat aaaaataaac aacaaaatac cgatggcgat tttcgtccac
    18061 caggaactca gcgtgccatc aaagtttatg taagtctgaa tcagtccctg aatcgccacg
    18121 ccaaaaagcg tccctaatac cgttccaacg ccaccactca aaagcgtacc gccaattacc
    18181 actgaggcga tagcatccag ttccacacct acgcccgcca gtgcatatcc ggcctgggta
    18241 taaatcgaga agacaatccc cgccagcgtt gccagtccgg tggagagcat ataaatgcga
    18301 atagtggtgc tgcgagtgga aatccccatc aggtttgccg acgttgcgtt gccgccaatg
    18361 gcgtatacct gattaccaaa acgggtacga tgcgcgagga atatgccgat aaccaccacc
    18421 gccagcatca gcagtcccat cgcacttaag cgaccgccgc cagggatttt ccacgcaagg
    18481 cttgagagcg tgtcataaac aggatggttt atcgggatcg actcttccga aacgagatag
    18541 ctgacgccac gcaaaaagaa cattcccgca agggtaatga taaacgccgg gatcttcagg
    18601 gcgtcaatca gaagccccat aaatgcgccg aaggcacagc ccatcaccaa caccagcgga
    18661 aacgccagca gcggcgagag gccgaaatcg ccaatcactt ttgccagaaa cacgccggta
    18721 aaggcgatca ccgaaccgac ggagagatcg atcccaccgg agaggatcac aaaggtcatg
    18781 ccaacggcga ttatcccaag aaaggcgtta tcggtcagga tattgcagat cactcttgtg
    18841 gaagcaaaac cgggaaactg ggtcagacag tagatataac ccaacacaaa gacgccgatg
    18901 gtgatcatca gtggcaaatt acgttttatc acggttacgt acccctttaa tcagactgat
    18961 aaagcgttgc gactgaacaa tcagcacgca aagcaccacc accgctttca ccacctggtt
    19021 catctccggc ggaaagccag aaagcaaaat tccggtgttc attccctgaa taatcagcgc
    19081 ccccaccacc gaaagcagca ggttaaagcg cccgcccatc agcgatccgc cgccaatcac
    19141 cactgcaaga atggcgtcca gctccagcca taacccggcg ttattggcat cggcaccgcg
    19201 aatatccgcc gccacgataa tgcccgcaat cgccgcacat agcccgctca acacgtaggt
    19261 gagcatgacg atgatccgcg tgtttacccc ggcatttttt gccgcccgaa tgttgatacc
    19321 aacggcttcg ataaacatcc ccagcgccgt tttgcgggtc aacagccaga acaggagaag
    19381 tgtcagcacc gcaataatga ctggtgttgg caggaacaac agcgatccac tgccgaacca
    19441 ggagagatcc ggtgagttaa atgtgacgat ctgcccggag gtgatcagtt gcgccacgcc
    19501 gcgcccggcg accatcagga tcagggtggc gacaaacggc tgaattttga ggatcgctac
    19561 cagtatgccg ttccacaatc ccgccaggat gccagtgccg agggcgctta acaaaacaat
    19621 tggaaggctg aatcccgcga ccgtcatcgc agccgttgtg gctccggcga tagccatcac
    19681 cgcccctacg gagagatcaa tcccaccagt ggcgataacc agcgtcatgc caatcgccag
    19741 tagcgcaacg ggggccgcac ggttaagaat gtcgataggg ctaccgaaca aacgcccatc
    19801 ctgaagcacc acctgccaga aatgcggggc caccaggcta tcgaccagca gcaccagcaa
    19861 cagtgccgcc agctgcggca ttcctgttgg ccagcgaaaa cgccttttcg gcggcgtagt
    19921 gtccgggaga gattgaggca tcacactgtt ctccttacgc cgcaatggcg ttcatgatcg
    19981 ccggaacgga aagctctgcc agcgggatct ccgccacctg tttgcgatcg cgcatgatga
    20041 tcacccgatc ggcatagccc accagctctt ccagctcgga ggagatcacc agcagcgcca
    20101 gcccatcggc gcacagcgtt tcaatcagcc ggatgatctc ggcgtgggca ccaacatcaa
    20161 ttccgcgcgt tggctcgtcg agaatcagaa attgcggtcg ggtcagtagc caacgtgaaa
    20221 gcaacacttt ttgctgattg ccgccggaga gaaattcaat cggttgttcg gtggaaggtg
    20281 tgcgaatgcc aagctggcgg ataaagcgtt cggcaatctc ttgctgctct ttgcgggaaa
    20341 taggtcgtag ccagccgcgc tgggcctgga gagcaagaat gatattttcc cgcaccgagg
    20401 cggcggcgat gatgccatcg gttttcctgt cttccgggca gaagccgatg cccagtaccg
    20461 aagcctgatg tggcgagcgt ggcgtttgtg gtttgccttt gatcaacgca gtgccgctgt
    20521 cagcaggttt gataccgaag atcacttcgg cggtttctgt ccgccctgat cccagcaagc
    20581 ctgccagacc gacgatctcg ccggggcgca cttcgagatc aaacggtgcg atcgttcctt
    20641 ttttgccgta atttttgaac gcggcaacag gtttgtcgct cagcaatgtt ctcccggcac
    20701 gctgaagcgc gtgggtgtcc agctcgcgcc ccagcatcat ttttaccagt tcgatctgcg
    20761 gtagctcgcg cgtttcccga cagcccacaa aactgccgtt acgtaagacg gtgatccgat
    20821 cgctaacctg ataaacctga tcgagaaaat gagtgacgaa gatcaggctg acgccgcgat
    20881 cgcgaagctg acgcatcagg ccaaacagta actctacttc ctgagtgtcg agactggcgg
    20941 tgggttcatc gaggatcagc actttggcgg agagatcgat agcccggcaa atggcgacga
    21001 tttgctgcat cgcgactgaa aagcggttga gcggttcgcg cacgtcgagg gagaaaccgt
    21061 aagatgccat cagttcggtg gcgcgctttt ccatctcttt acggcgtaga aggccgaaac
    21121 gtttgggttc gcgacctatg aatagattat cagcgaccga catattgggt agcaggttga
    21181 cttcctgata gacggtgccg atgccgagtt gttgtgcgtg ggcggtgttt ttcggtgaga
    21241 tagcctggcc ttccagccag atagtgccgc gatcggcgtg gtatacaccg gttaatgctt
    21301 tgattagcgt cgattttccc gccccgtttt caccgagcag cgccatgatt tcgccacggc
    21361 gcaggctgaa attaacgttg tctaacgctt tgacaccggg gaaaaattta cttaatcctt
    21421 cggtgcggag gatttcctgg tgttggtcgg tcgtcatggt ttccccctta gccacgctct
    21481 aaaagtccgg gaatagatcg tgcctgatgt taagagctgc gccgtttgtc cccctcaccc
    21541 taaccctctc cccaaagggg cgaggggaca gattgtgcat ggtgttaagt ccggtactgc
    21601 acaactgccg gatgcggcgt aaacacctta tccggcggcc cacaagtcgg catcagatat
    21661 tgatgcccct caatacccca tgtttttctt cttctctaac tcttcttttg cggtatcagg
    21721 caggtaaagg gtggacttgg ttaacgtcag cttttcaggc atggtgccgt cttttttgta
    21781 tttctccagc gcgtcaaaag cggggccagc catgttcggc gtcagttcaa cgctggcgtt
    21841 tgcttcgcca tcaatcatcg ctttatagat atctggtacg ccatcaatgg aacccgtgag
    21901 gatatcttta cccggcttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat
    21961 gtcgtcgtta tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa
    22021 gctctccatg acttctttgc ctttactgcg ggtgaagtca cctgactgcg agcggataat
    22081 tttgatattt ggcgcattct ttatggcttc ggcaaagcct ttcttacggt caatggcgac
    22141 gctggcccca acggtgccct gcagctccac cacgttacat ggtttgccgt taacctcttt
    22201 caccagccag tcaccgatca acttaccttc gaggatgttg tcggcagtaa cggtggtcat
    22261 atagagagat ttgtctttca catcgatgga acggtcaagc aagaagaccg ggatttcggc
    22321 atctttcgcc tcttttaata ccggttccca acccgtcgca accaccggag cgataaagat
    22381 cgcatccacc ccttgtgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg
    22441 ctgaccatcg gcaattttca gtgtgatacc gcgcttttcg gcttcacttt tcgccacatt
    22501 ggtttctgcg gcgcgccagc cggattccga tccgacctgc gaaaatccta cggttaatgg
    22561 agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt
    22621 ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa
    22681 ctatagacaa ttcgttatgt aacggattgc gttacataac acttcaaaaa agtaaataaa
    22741 acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat
    22801 ggattttcct gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa
    22861 caagcgaaga cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt
    22921 ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac
    22981 atctacgttg ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa
    23041 gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac
    23101 tacggttaca tcaaccacac tctgtctctg gacggtgacc cggttgacgt actggtcccg
    23161 actccgtacc cgctgcagcc gggttctgtg atccgctgcc gtccggttgg cgtactgaaa
    23221 atgaccgacg aagccggtga agatgcgaaa ctgattgctg ttccgcacac caagctgagc
    23281 aaagaatacg atcacattaa agacgttaac gatctgccag agttgctgaa agcgcaaatc
    23341 gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggttaa agttgaaggt
    23401 tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgccaagaat
    23461 aaataagttc ttctggcgca ataaccctga acaccgggct tcggttagta agggtttttt
    23521 tatgcccgca atttataaac tctctactcc tattattatt ttcagcagtt gaaaggcttg
    23581 aacggtaaga acaagctaag ctcgaccacc attttgctgt tcatagccac ttgctggaag
    23641 ttagccggcc tcactcatac tcaccgttca aagcgtgtcc ccgttttacc cctgtacatt
    23701 tgttaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggcc cggagagaaa
    23761 agcaaaagat gggaaagtga caaaaaaaat cgcgagatac atcgcataaa acaacgccac
    23821 ccgaaggtgg cgtgctttta ttattactta tccctgtcta accagtcgcc gctttcaatt
    23881 agctttaatc catcgacggg tcgttgataa acgtacatcc atgcactccc gtacggcgtc
    23941 tgaatcaact ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag ttcggccagc
    24001 gtggcgttgt caatacgata aacttcaccg tgtactgttc cgttccccgg aactgcgcct
    24061 ggatagtggc ccaggctata caactggtag ttatcgatac tgaaatcgcc cagtaactgg
    24121 gcattggtca tccagtgact gttgccttgt ttgtggcgta aactgccgta gacaaatatt
    24181 cgcattgcta aaactcgaac tgatagagca aatccagtgc ctggtctaca ccagacacgg
    24241 cttccagata tagcttaggc atcaggcgat aacgtaacgt gagtgttgct atagagtcaa
    24301 atatacccac gccgtatttc acttgcagac ctggcaatac atagccgctg accaccacct
    24361 gggaggagtc gcctactccc tctgtgtcga gcgctaaatt gcttacgcca aacgtctcgc
    24421 cgattttacc cacaatctgg ccactttgcg caacccccag gccaatcagc atcgaggtca
    24481 tcgccgcact gtcactctga tcgctctcca gcccttgtcc acgtagcaaa taagacaatg
    24541 cagcttgttg cgacatcgcc gggtcagaga agatctccgc tttcggttcg tccgccagac
    24601 cagtgacgcg aactccggcg atgacgtcgt cttctgtagc atccgggtta cgaatagctt
    24661 cgatattaag atacggttga tctggcggac cagagaacag taactcaccc ttacgcacaa
    24721 tcagatcctg accataggca tggaagcgcc cttcagggat gttgatctgc ccgttcaggc
    24781 ccagcccttg tttgtcctga acgacgttga gatcgcccgt cagccgcgct ttcaggccaa
    24841 aggcgtcaat gcgcacattg ttgccaacgt ggacagtcag gttactgtta atcggaatcg
    24901 acgccgtttt cggctcttcc ggttgcaggt tatcgttaag catcaccaca tcgctggaaa
    24961 cgcctactgc gctttccggc agctcgtgca ccacgatgcg cgcccaaggg acatccacgc
    25021 gaccgtcgag agtaaacaga tttggtgtag cctcgaatac aacatctggc gatacatcca
    25081 ttcgtaccat cggcggcacg gtgatacgca ctttactgcc cttcgctgtt accctcgccc
    25141 gccagttttc aatctggctc cagtcggcgt caccgctcag gtagatttct ccctgttggg
    25201 tccgcacagt accagcaagc gtcgagcgca taccgttaaa gttgaccgca agctggctcg
    25261 gttgcatatc aaacggcata aagttgccgt cgatatccac ccccgtaacc tgaagctgac
    25321 caaacaactg cgggctttgc acatcaccac ccagacgcaa gttggcactc accatccccg
    25381 ctgctttttc cccacgggta aagatggggt ttatcatcgc aaggttgaag ttacggatat
    25441 tgacgttgcc accaagatta cggcggcctt gcggatcggt cacctgcacc tgaccatcaa
    25501 actggccgtt attggtcagg cggatggtcc agcccagttc ggcacggttg ttacgcagtt
    25561 ccgccgtcag attcagcgtc tgaaacgcca cgggcagcgc cgcatcgttg acggtttgcg
    25621 tcacctgcac attacgcccc gaaagggtga tactgccctg cggcagcccc tctttcgtgg
    25681 tgtcccaggc aacgtccgct ttacccgtga agataccgct ggcctgagtg gtttctggca
    25741 taaatggttt cagcatggcc aggtcgaagc ggttgagatt caccaccgca cgcccttcgg
    25801 cacccgcatc gatagtttgc ggcacgcaca gttccgcatt cgggttaagc cagcagtgtg
    25861 gcccgatgct gattttttgt tccttattgc ggtaatccag cgcaatatcg cgggtcagcg
    25921 accacggacc gaccggcgtc tggaagcgcg tattgctaag tgttcccttc cagcgttctt
    25981 ctttgcgatc aaaacttcct gccagattaa gctgcccgga aatcggttcg ccctgaatcc
    26041 gcaactgtag ctcgtgctgc ttttcgctgc ctttggcatt cagggtgacg aggttgatat
    26101 ttacatccgg ctgcgagatt tgctcaacgc gtacgtcgag tttcccggcg atctgatcgg
    26161 tggatttaat gtcgccttcc acgcgaacct gcgccacgga aagttcctgc cagcgcaggc
    26221 cgcgcgcggt gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt actttcacca
    26281 gccctttcgc tgtaccgcca agccccggca gcgcgttatc cagccccggc gcgttgatgg
    26341 tggcatcaag attgagatct tttaccccca gctcaccttt cacttcggca ctgtttggcc
    26401 ccagttccag atgcagccct gggatcatcc actgcatata actgttgcct ttcagcgtgc
    26461 cgtcaacgtt cactttgttc tgtttgacgt taccggtcag cttcagttct ggcacctcca
    26521 tctgccaggt gccgccgtac aggctaccgc gggttttaat caagccattg agtttcgacg
    26581 gccactccgg gatctctttg gcggtgttaa tgccgttaag cgttagctca ccgcgccaac
    26641 taatggcctg ctgccagtcg agcaacgcct taagttccgt tttcccttcc agcgccgcga
    26701 cggtaagttt gtcgagattg acctgctgtt cattaccttt ggcatcaagg gtaatggtgg
    26761 ctggcgggat ctcctggccc ttaacagccg tgcgcataga gatcgcgtaa tcggtcattt
    26821 tgccggtaag tttcagtttc agatcgtctg cctgatactg cttctcacca gtgaacggcc
    26881 agtaaagctg tttgctgttc acttccacgt tgagcggcaa tccggcttcc gccagtcgcg
    26941 tctgggcgcg taaatccata tccaccggac cggaaaggtt aacgcctatc tccagctgtt
    27001 cgcgcagcgc accgcccact ttcagcttca ccttttcacc tttcaacggc tccacgttca
    27061 gtgtgctgtt gagggtgata tccaccggcc agttgtctga cagctgcgcc gtgccgctgg
    27121 cgttgacgat cccttgactg gagtcgatat ccagggcgtc cagtttagta ttgccgtcaa
    27181 tgctgctcac tttcagcagc atggtgctca cggtgatgtc cgtgtcgccc gtcacgcgca
    27241 gctgctcgcc cttaaactct tcaatgttca ggttaagcgg caaatgcacg tcggtcattt
    27301 ccggcaatac cgggcgagaa aagagatctt tcagcgtttc gccgagcggc ttgtcctccg
    27361 gctgcggatt ttcaattttc ggttcgacca cttcttcctg tgccgcttcc gccactttcg
    27421 gcaaagcaat caacaggcct atcagcgatg tcggtttcag ggtcagggtt ttctcctgcc
    27481 agttcaggcc ggaggtgaag tccatcaccg ataccgtggt gtcatcaatc ttgatgttga
    27541 cgttgtccag cgccacccgt gtcagggtaa tgggatacgg cgtggagaga tccaacggac
    27601 cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg tcgatgttga
    27661 cctgaatgtc tttcagcgcc agatcattaa tacaaacgct actgttccac aggcactcaa
    27721 gcccgaccgc cagatgcaga ttgcccgctt ttaccgccac gcctggctgc tcataacgaa
    27781 cgtcagacaa ggtgagatca cgccagccgc cggtgacctt gccaatatcc agtcctggca
    27841 cccagcgatc cgccgcttta aataccagat gcaggccgct ggtggtgccc accagaaacg
    27901 ccaccgatcc cagtaacagt aagataacga taaccacgcc gaggctgatt tttttccata
    27961 aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt ctttatccgc
    28021 gaccggcacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc gcacgccgac
    28081 ccctgtaccg gttttaaagt cgctgcggcg aatatcgctt accgcttcgc cgctatcgac
    28141 aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tattccagcg atccggttat
    28201 cagcttcgag gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag atttgtattt
    28261 gtagccgcga atactgcgat cgcccccggc gaagaaacgc agatccggcg gtactttgtc
    28321 gaaatcaccc gtttcaatcc agcccagcgt gccgcgtgtc acaaaacgat ggcgatcgta
    28381 cagtgtgcgg atccagacgt tctgcgcctg gaaaacggag aaatcaacat ccgagcccca
    28441 ggccgtgttg gagtagtcga tagagtagcg ttgcgagtcg ccccaggttg gcatcaggcc
    28501 accacgagaa cgcgtgcggc taatcatcac cccaggataa aacagcatcg tggtgttggt
    28561 aatttccccc tgggtaaagt ggtcgaaact ccagcgcagg ttaatggcac gctgccagcc
    28621 gctggagaga tcccagtagc gagaagccac cagcgtagtg gagtcagact cggtatcgtt
    28681 caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagcg gattcttcag
    28741 cagcggcatt ttatagctga agtcgaggat ctgttccggc gcggaaatac tggtactggt
    28801 ggtcaaactg tgaccgtaag agttcatcca cggctttttc cacgtcgctt tcacgcgcgg
    28861 tcccacgtcc gtagagtaac cgacgccggt ttcgatggtg ttttctgttc gcggcgaaac
    28921 cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg gagccaccac
    28981 caccgagtta aaccagccgg tagcagaaag tcgacggttc agttctgcca gatctttcga
    29041 ttcgtactca tcgccctctt taaacggcac cagattttgc aggtattcat cgcggatttg
    29101 tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat aatcaatatc
    29161 ccagaaggct ttatgcaggc cgagcgcaat gcccagctgt gctttggtaa attcgctatc
    29221 gaaataacct ttacgcaacg caatgctggt taaggacttt ttgaaatttt cataatcgcc
    29281 ctggttcagt accgtaccaa tagccgggcg agtatcgagc aatttcaaat agtctttatc
    29341 ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg gcacgcctgg
    29401 cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga gatcaaattc
    29461 aatggtcggc tggtaataac ccagcgcttt cagaccttcg cggatggcat catcgacgcg
    29521 tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa gctgcgcgcg
    29581 aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt tcgcggcgac
    29641 ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat agcgcacatt
    29701 ttctcctgaa tatccttttt ttcctgcccc tggaacgccg ttaaacggct taacaaaaat
    29761 ccagtaatat ggattaaaaa gcagactaaa cccaaaatat ttcttatgtt ttactttaga
    29821 cctattcacg gtggttattg tgtgcaaata cgcctcttgt tacaacctta accccaatga
    29881 ccgattttcg ggagagcgac accatgagtt tatttgataa aaagcatctg gtttcccccg
    29941 ccgatgccct gcctggacgt aacaccccga tgcccgtagc cacactgcat gcggtcaacg
    30001 gtcactcaat gaccaatgta cctgacggaa tggagattgc catttttgcg atgggttgtt
    30061 tctggggtgt ggagcgtctg ttctggcagt tacccggcgt ttacagcacc gccgcaggct
    30121 ataccggcgg ctatacgcca aatccgactt atcgggaagt gtgctccggc gatactggcc
    30181 atgccgaagc ggtgcgcatt gtttacgatc catccgtcat cagctatgag cagttgctac
    30241 aagtattttg ggagaatcac gatcccgccc agggaatgcg tcagggcaat gaccacggca
    30301 cgcagtatcg ttcagcgatt tatccgctga ccccagaaca ggatgccgcc gctcgcgcca
    30361 gtctggaacg ttttcaggcg gcgatgcttg ccgccgatga cgatcgtcgc atcaccacgg
    30421 aaatcgctaa cgccacaccg ttttattatg ccgaagatga ccaccagcaa tatctgcata
    30481 aaaacccgta tggttactgt ggaattggcg gaattggcgt ctgtctgccg ccggaagcat
    30541 agcgttacgg gtacaaatgt agattgttga taaagtgcat tttgtttatg ccggatacgg
    30601 cgtaaacgcc ttgccaggcc tacaaaatcg tccaaattca atatattgcg gggactgcgt
    30661 aggcctgttc ggcatagcgc atcaggcagt ttttcatttt tcagctgtct gaattttaac
    30721 cctctggcgg ctttacagta ccttacgcta tactagccac tgaaaatgcc ggttcacttt
    30781 cttcgaatcg gctttcaatg tgtatttcac acaaattaat caacttcccc ttccgaggat
    30841 ctggcctgaa aggtcggata agatatgtta aacagtattt tagtcatact ctgtttgatc
    30901 gctgtaagtg cgttcttctc gatgtccgag atctcgcttg ccgcctcacg caaaatcaaa
    30961 cttaaactgc tggctgatga aggcaatata aatgcccaac gcgttctgaa tatgcaggaa
    31021 aatcccggca tgttctttac cgtggtccaa atcggtctga acgcagtggc gattctcggc
    31081 ggtatcgtcg gtgatgcggc attttctcca gcttttcaca gcctgttctc ccgctatatg
    31141 tcggcagaac tctctgagca actgagcttt attctctctt tctcgttagt gactggcatg
    31201 tttatcctgt ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc agaagctgtg
    31261 gctttgcgta tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc gctggtgtgg
    31321 ttcttcaacg gactggcgaa cataatcttc cgtattttca aactgccaat ggtacgtaaa
    31381 gatgacatca cttctgatga catctacgcg gtagtggaag ccggtgcgct ggcgggcgtg
    31441 ttacgtaaac aggaacacga gctgattgaa aacgtctttg aactggaatc ccgtaccgtt
    31501 ccgtcttcaa tgacaccgcg tgaaaacgtg atttggtttg atctccacga agatgagcaa
    31561 agtctgaaga ataaggtggc ggaacatccg cactctaagt ttctcgtctg taatgaagat
    31621 attgaccaca tcatcggcta tgtcgattct aaagacctgc tgaaccgcgt gctggctaac
    31681 caaagcctgg cactgaacag cggcgtacaa attcgcaata cgctgattgt gccggatacg
    31741 ttaacccttt cagaggcgtt ggaaagtttt aaaaccgcag gtgaagactt cgcggtgatc
    31801 atgaacgagt acgcactggt agtggggatc atcaccctca acgacgtgat gaccacgctg
    31861 atgggcgatc tggtcggtca ggggctggaa gagcagattg tcgcccgtga tgagaactca
    31921 tggctgatag acggcggcac tccgattgac gacgtcatgc gcgtgctgga tattgacgag
    31981 ttcccgcagt cgggcaacta cgaaaccatc ggcggcttta tgatgtttat gctgcgtaag
    32041 atcccgaaac gcaccgattc ggtgaaattc gccggctaca aatttgaagt ggtggatatc
    32101 gataactacc gcatcgacca gctgctggtg acccgaatcg acagcaaagc caccgccctt
    32161 tcgccaaaaa tgcctgacgc taaagataaa gaagaaagcg tcgcataacc ccagaaacat
    32221 caacggctcc tgaatcagga gccgtcttat tactgcatag cactttggtt aagccatctc
    32281 tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat gcagcttatc
    32341 cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa cgtccttgat
    32401 gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg ggttgtaccg
    32461 ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctttgcggtg ccacaatcgg
    32521 cccaactatt atgaggcgca aattttaatg cctagtgact atagactatc cgggcaatgt
    32581 ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt atctttttac
    32641 cgaagcgcgg tcttcagtat aagcattcat ttttaatatg atttgtgcgc ttgaccgcaa
    32701 actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa ctcagaccca
    32761 agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg caagattctg
    32821 gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga aaccggtcag
    32881 cgagtgccgc cgattggcat caccgatcgt ggcgagttgg tgcttgataa agatcagttt
    32941 agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact gcaacatatt
    33001 gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat taaatcagcg
    33061 aagttaccgc acgatcgtta ccagaccacc accattgtta acaccgacga cgcgattccg
    33121 ggttcaggga tgtttgtgcg cagcagtctg gagagtaata aaaagcttta tccctggtcg
    33181 cagtttattg ttgatagcaa tggcgtcgca cgcggtgcct ggcagctgga tgaagaaagt
    33241 tccgctgtag tggtgctgga taaagacggt cgcgtgcaat gggccaaaga cggggcgctc
    33301 actcaagaag aggtgcagca agtgatggac ctgctgcata aattaattaa taaatagaca
    33361 ctctgaaccc cggattcagg aacgactcgc gcggagtgta atccagcggt ttaccttgcc
    33421 agtcgtgaac gtgcgctccg gcagctgcag ccacagcatg accagcggcg gtatcccaaa
    33481 tattcgttgg cccgaaacgc gggtacagct gcgcctgtcc ttccgccacc aggcagaatt
    33541 tcagcgaaga gccgatggac gtggtctgat gttcgccaag ctgttgcaga tactctttca
    33601 gctccgcatc tgcatgggaa cggctgatca ccaccagcgg cgggcgcgca tcgcggacct
    33661 gaatctgctt gcgcacaccg cactcttctt tccaggcttt gccttctgcc gcgctgtaca
    33721 ttacgttcat taccggcgca tacaccacac ctaaaatcgg tttgccgtgg tcgatgagcg
    33781 caatgttaac ggtgaattcg ccattacgtt taataaactc tttagtacca tccagcgggt
    33841 ctaccagcca gtaacgctgc cagtgctgac ggacttccca accgggagga tcttcttcag
    33901 aaaggaccgg aatatccggt gtcagcgtac gtaaaccgtc catgataacg gtgtgagcgg
    33961 caatatccgc tgccgttacc ggagaattgt ccgctttgct gacgacgtcc atcggttttg
    34021 tcccgtcgta gacctgcata atggcatcgc ctgcattccg tgcaagctgg catacttgat
    34081 ctaacatttc tccacctcgt ctctgtgagc ggtgttaact tattgtttta cttataccct
    34141 atcgttaatg aatgcgccaa ctgtgatagt gtcatcattt tcaaagcgta aaattgtggc
    34201 attcttcact gttctataag taagacgttt attctccctt ttctttcgta ttcccgatga
    34261 taaaaggatg tccctgatga ttaagtttag cgcaacgctc ctggccacgc tgattgccgc
    34321 cagtgtgaat gcagcgacgg tcgatctgcg tatcatggaa accactgatc tgcatagcaa
    34381 catgatggat ttcgattatt acaaagacac cgccacggaa aaattcggac tggtacgtac
    34441 ggcaagcctg attaacgatg cccgcaatga agtgaaaaac agcgtactgg tggataacgg
    34501 cgatttgatt caggggagtc cgcttgcaga ttacatgtcg gcgaaaggtt taaaagcagg
    34561 tgatattcac ccggtctata aggcattaaa cacgctggac tataccgtcg gcacgcttgg
    34621 caaccacgag tttaactacg gtctggatta cctgaaaaat gcgctggcgg gagcgaaatt
    34681 cccttatgta aatgccaacg tcattgacgc cagaaccaaa cagccaatgt ttacaccgta
    34741 tttaattaaa gacaccgaag tggtcgataa agacggaaaa aaacagacgc tgaagattgg
    34801 ctatattggc gtcgtgccgc cgcaaatcat gggctgggat aaagctaatt tatccgggaa
    34861 agtgacggtg aatgatatta ccgaaaccgt gcgcaaatac gtgcctgaaa tgcgcgagaa
    34921 aggtgccgat gtcgttgtcg ttctggcaca ttccgggctg tctgccgatc cgtataaagt
    34981 gatggcggaa aactcagttt attacctcag tgaaattccg ggcgttaacg ccattatgtt
    35041 tggccatgct cacgccgttt tcccaggtaa agattttgct gatatcgaag gggctgatat
    35101 cgcgaaaggc acgctgaatg gtgttccggc ggtaatgcca ggcatgtggg gcgatcatct
    35161 tggtgtggtc gacttacaac tcagtaatga cagcggtaaa tggcaggtga cgcaggcgaa
    35221 agcggaagca cggccgattt acgacatcgc taataaaaaa tccctcgcgg cggaagacag
    35281 caagctggta gaaacactca aagccgatca cgatgccaca cgccagttcg tcagcaagcc
    35341 aatcggtaaa tctgccgaca atatgtatag ctatctggcg cttgtgcagg acgatccgac
    35401 cgtgcaggtg gtgaacaacg cgcaaaaagc gtatgtcgag cattacattc agggcgatcc
    35461 ggatctggca aaactgccag tgctttcagc tgccgcaccg tttaaagttg gtggtcgcaa
    35521 aaatgacccc gcaagctatg tggaggtgga aaaaggccag ttgaccttcc gtaatgccgc
    35581 cgatctttat ctctacccca atacgctgat tgtggtgaaa gccagcggta aagaggtgaa
    35641 agagtggctg gagtgctccg ccggacagtt taaccagatt gatcctaaca gcacgaaacc
    35701 acagtcactc atcaactggg atggtttccg cacctataac tttgatgtta ttgatggtgt
    35761 gaattatcag attgatgtta cccagcccgc ccgttatgac ggcgagtgcc agatgattaa
    35821 tgccaatgcg gaaaggatta agaacctgac ctttaacggc aagccgattg atccgaacgc
    35881 catgttcctc gttgccacca ataactatcg cgcttacggc ggcaaatttg ccggtacggg
    35941 cgacagccat atcgcttttg cttcaccgga tgagaaccgc tcggtgctgg cagcgtggat
    36001 tgctgatgag tcgaaacgtg cgggggaaat tcacccggcg gcagataaca actggcgttt
    36061 agcaccgata gctggcgata agaaactgga tatccgtttc gaaacctctc cgtcagataa
    36121 agccgcagcg tttattaaag aaaaagggca gtatccgatg aataaagtcg cgaccgatga
    36181 tatcgggttt gcgatttatc aggtggatct gagtaagtaa aacacttctt tttcggccta
    36241 caaatcatca accgcatccg gcatttattg gcggatgcga tgatgacgca tcttatccgc
    36301 cctacaagcc atgaaccgta gtccggataa gcgcagcgca tccggcagtt atgccgcacg
    36361 ttcatcccgc accgctaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc
    36421 aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac
    36481 caccggatac gcgatacggt taagaaaacc atcctgctct aacgcctgta acgactgcgt
    36541 aagcatcttt tcactcaccc cgcccatttt acggcgcagg tcgctaaagc gatgagtacc
    36601 ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc
    36661 gcgcgacggg cactgttccg caaagagatt tccctctttc agttgttgcg acaggctaac
    36721 ctgactcatt tcatacttac ctttttgtat gtacttacta aaagtaagtt taagtgttag
    36781 cgtatttaaa cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac
    36841 tggccaactt ggtcactatg ttattgaatc cttgatgaaa acggttcctg ccagccaaat
    36901 agtggctatc gttcgtaatc cggcaaaagc acaggcactg acagcacaag gcattaccgt
    36961 gcgtcaggct gactacggcg atgaagccgc actgacatct gcgcttcagg gagtggaaaa
    37021 actactgctg atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat
    37081 taatgccgca aaggcggccg acgtgaaatt tatcgcttat accagcctgc tacatgccaa
    37141 ttcctccccg ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc
    37201 tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagt gaaaactacc tcgccagcgc
    37261 cccggcagca ctggaacacg gcgtatttat cggtgccgcg ggcgatggca aaattgcctc
    37321 agcaacgcgg gctgattatg cggtagctgc ggcacgcgtg attagcgaag ccggtcacga
    37381 aggcaaggtt tacgaactgg cgggcgatag agcctggacg ctgacacagt tagcggcaga
    37441 actcaccaaa cagagcggaa aatcggttac ctatcaaaat ctgagcgaag ccgatttcgc
    37501 tgcggcgctg aaaagcgtcg gactgcctga tggactggcg gatatgctgg cggattctga
    37561 cgttggcgca tcgaaaggcg gtctgtttga tgacagcaaa acgcttagca aattgattgg
    37621 ccgcccaacg acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt
    37681 aattaaagtg gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat
    37741 gtctgcttcc tgaatatttc ttctgacaga ccagtggatg ccagtgatga ttagcggcgt
    37801 gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt
    37861 gccggaatac ccggcgatgt tacagtcgat ggggcgttac ctggcgctag ggttaattgc
    37921 gctgcccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gcgactggct
    37981 taccgccttg atgctcacca tgatgggcaa ccttatctat tacttctgcc ttgccagtgc
    38041 cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat
    38101 tcccgttttt gccaatctgc tttatagcca gcgcgacggc aaactcgcat ggggaaaact
    38161 cgccccggca ctggtttgta ttggcatcgg cctggcgtgt gtgaatattg ctgagttaaa
    38221 ccacggactc cccgattttg actgggcacg ttatacctca ggcatcgtgc tggcgttagt
    38281 ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc
    38341 cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct
    38401 catcggctat ctcgtcgcct gttactggct gaatatgcaa acgccggact tctccttacc
    38461 ctttggcccc cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc
    38521 atgggttggc gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg
    38581 gccgctgatt gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca
    38641 aatgccgccg ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcattgc
    38701 ggtcagagca aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacacgctgg
    38761 cagtgagtta aataagcctc tgctacgtaa gggttatagc ttttgcctta aagatgcatt
    38821 taaaatgcat cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct
    38881 ttaggtgaac tggcgctctc tattcctcgc gcttcggctc tgtttcgtaa atatgatatg
    38941 gattactgct gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat
    39001 gttgacgtca ttgaagccga actggcaaag cttgctgaac aaccgattga gaaaaactgg
    39061 cgtagcgccc cgctggcgga aatcatcgac cacattattg tgcgctatca cgatcgccat
    39121 cgcgagcagc tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac
    39181 aaaccaagtg tgccaaaagg gctgacaaaa tacctgacta tgctgcatga agagctttcc
    39241 agccatatga tgaaagaaga gcaaatcctc ttcccaatga tcaaacaagg catgggcagc
    39301 caggcaatgg ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg
    39361 gaagtgatca aacacaccac caataacgtc acaccgccgc cggaagcctg caccacctgg
    39421 aaagcgatgt ataacggcat taatgaactg attgatgacc tgatggagca catcagtctg
    39481 gaaaacaatg tactatttcc gcgcgcgctg gcgggtgagt gatctaaaag ctggatggca
    39541 ttgcgccatc cagcatgata atgcggttat ttccgcagtt cagcagcctg cttcttacca
    39601 ataaacagcc agcccagccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg
    39661 cgggtgtcat cttccagtgt caacaacacc agaacaaaca caaagaacgc catacatacc
    39721 cagcacatca gcttgccgag tggcatcttg tagatcgact tctcatgcag atgaggacgc
    39781 tgtttgcgat acaccaggta cgagcaaagg ataatcgtcc agacaaacat aaacagaatc
    39841 gcggaaacgg ttgtaatcat cgtgaacgcg ccaatcacgc tgggattcac atacaacatc
    39901 accacgccgc cgagcagaca gatacaagag aaggtcagcc ctttcgcggg tactgcgcgc
    39961 ttagagagtt tagcgaacgc tttcggtgcc acaccttcct gggccagacc aaacagcata
    40021 cggctggtag agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag
    40081 ttgatcacgc tggcagccgc aggcaaacct accaacacaa acagttcaac gaacgggctt
    40141 ttctccggga ctaccgaact ccacggcgtc acggacataa tcacaatcag cgagaagacg
    40201 tagaacataa tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga
    40261 tctttggttt ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc aaaaaccgct
    40321 atctggaatc cggcaaagaa gccgcttaag cctttcggga accagccgcc gtcattccac
    40381 aaatgtgcaa atgatgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc
    40441 aggccgacaa caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc
    40501 atctcaccga acatttttac cgtggcgagg tttagaacca gtaacagaat gatcacggat
    40561 aacgaagcaa cccagtcaga aagcccaggg aaccagaatt gcgcataggc ggtaatggca
    40621 accacatctg ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat
    40681 cctgcccacg gcccgagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc
    40741 gaaagcagca attcccccat tgcccgcatc acgaaaaaga gcataaaacc gatgatcata
    40801 taaacgaaaa tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac
    40861 cctgtaccaa tggcaccgcc aatggcaata agctgaatat gtcggtttgt gagattgcgc
    40921 cgtagcgact gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg
    40981 tttttttctt cctgtacctg tctgtgttgt tcaggctcta cggcctttag tcagtctatg
    41041 atacgacgaa tctgtatcgg gactcatcga tattaggtaa caatacgcgg gatgaatact
    41101 aagatttaga tctaatgtta attttatgtt taaagtgagt gtcatatcac ggtgataacg
    41161 cgaaacagct cacaaaaata cacgcttaca gggtctttgc aacataaaat agcgccgttt
    41221 ggcaaactga agggtttatt gccgaatgcc tgctcccctc tggttcgagg ggaacagaat
    41281 gcgctgctta gaggatttcc agcagttcga cttcaaacac cagggtgctg aacggaggga
    41341 tggacgcgcc tgcgccacgc tcgccatatg ccagttcctg cgggatagtc agttcccatt
    41401 tagaacctac cggcatcaga gtcagtgctt caatccagcc agggatcacg ccgtttaccg
    41461 ggaattcagc tggctcgcct cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt
    41521 ttccggtgta atgaacacga acgcggtcgg tacgtgccgg aattgcgcct tcgccctggt
    41581 tgatcacgcg gaattgcagg ccagattcgg tgctatttac accttctttt ttggcgtttt
    41641 cttccaggta tttcacacct tcagcagcca tcgcctggaa acgctgacga cgaacggcat
    41701 cggcgcgttc gtggatttca cgcagcgcgc ggtgcaccac atcaaccgga acagccggat
    41761 gtttgccttc cagcgcatcg gcaatgcctg caaccagtgc ttctggcagc agcccttcca
    41821 ggccagattc actcagctgt tgccctacct gcaaaccaat gccgtagctt gcttgcgctt
    41881 cgatggtgtc aaaagttggg gtggtcatcg tatttccttt cttagggtat aaagtagcgg
    41941 gcagcatatc agccacactt ctttgggtaa aactttgtcg cgggaatgat gacaaatccc
    42001 ggggaaaaag aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg
    42061 acatcatagc gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg
    42121 aaaccatgcc cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg
    42181 acaattttcg cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg
    42241 ccattgtatt ggtagtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg
    42301 tgacgcgtga agcgcagtta gatattcagt cgcaatcaca accaccgaca gaagaacagc
    42361 tgcgggcgca attagttacg ccacaaaatg atcctgatca ggtggctccg gtcgctccag
    42421 aaccaataca ggaaggccaa ccagaagagc agccgcaaac cacccatacg caaccattcc
    42481 agccagatag cggaattgat aatcaatggc gctcctatcg tgtcgaaccc ggtaaaacga
    42541 tggcgcagct gttccgcgac cacggactgc ccgctaccga tgtctatgcc atggcgcagg
    42601 tagaaggcgc ggataagcca ctgagtaatt tgcaaaacgg gcagatggtg aaaatccgcc
    42661 agaacgccag cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc
    42721 tgtttacccg ccagccggat ggcagtttta ttcgcgcgcg gtagatgaag ggtcaaccaa
    42781 aagccagcta aggattgtct ctttgatgac cttacgtctg gcttccagtg cctgcggtga
    42841 aaccatactg atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc
    42901 aagcgcagca atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattttg
    42961 tttcttccct tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa
    43021 aagacgctgt gattttgcat aatgcacacc cttactttga ttttcactat gcacaatttt
    43081 taaaaaccag ggattagaaa tataataatc ccatgtaaaa tcgattattt tctcaatggc
    43141 tttctgtggc ggaagatctg agaaatcaag ggccttttct ttttgtcgaa tatcgttcca
    43201 ggtatattcc agtacttcga taaataaatt ttctttatta ccaaagtggt gataaaccgt
    43261 ctgtttactg caccccgcgg ccttgacgat attatcgact ctggcccctt cgtaaccata
    43321 ttcggcaaat tcattaaccg cgcataacag cagcttttct ttcaggctct gtacgttgct
    43381 ggtatcaaga tacataacgc ttccccacga cctcttcgtg gtgactatca taacctgatt
    43441 tgatttcagc ttcagaaaaa agggcaaaaa cttgtacttt catcaggtta tcaaatgtcc
    43501 tgtgatcgcc atcgcatatc aaccaactag atggttgatc tttttatcct ccagtcgcaa
    43561 gctaatcatc actaaggaaa caaaattcaa aaaaagagag ctattgtatg aaatccactc
    43621 gtcctgttgc cgtgattacc ggagccgctc gtggtattgg aaagggctgt gcgctggagc
    43681 ttgcgcgtgg cggatttaat ctgctgatta atgatcgtcc ggatgcggat agcgtagaaa
    43741 aattacatgc cacacaacaa gaatgtctgg cggaaggcgt ggaagtgatt tgcttccctg
    43801 ctgatgtggg cgatctctcc ctgcatgaag agatgctcga cgcagcgcaa aaccagtggg
    43861 gacgtctgga ctgcctgctc aacaacgcag gcatttcagt gaaaaaacgc ggtgaccttc
    43921 tcgacctcga accagacagc ttcgaccaaa atattgccat taacacccgc gcaccgttct
    43981 tccttgctca ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcagaactgc
    44041 cgcaccgctc gattattttt gtcagttcca tcaacgccat catgctggcg atgaatcgcg
    44101 gcgaatatac cattgccaaa actgccgtct ctgccgctgc tcgtctgttt gctgctcgcc
    44161 tttgtaatga acaaattggc gtatatgaag tgcgtcctgg tctcatcaaa accgatatga
    44221 ctattccggc aacggcctat tacgacgaac tgattgccaa aggtctggtg ccgtggggac
    44281 gttggggtta tccggcggat attgcttcca ctgtccgcgc gatggcggaa ggtaaactta
    44341 tttacacctg tggtcaggcg gtcgccatcg acggcggctt aagtatgccg cgcttctgag
    44401 gctgaatcat gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac
    44461 tgggctataa aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg
    44521 cgattgcccc ctggcgcagg gagttgcaga gtgaagatct ccagcaaacc gcccgccagc
    44581 ttgctgccag taacatgaac gtttccggtt tgtgccgtag cacctactac accgcaccga
    44641 cgctggcgga gcgcaaactg gcgattgacg ataaccgccg ggcgctggac gacgccgctg
    44701 tactgaacgc cgcctgttat atgcaggtgg ttggcggtct gccaatggga acaaaagatc
    44761 tgtacgaagc acgggaacag gtgaaacaag ggattcgcca gttgctgcca cattcgaaag
    44821 aggtcggcgt gccaattgca ctggagccac tgcacccgat gactgctgcc aatcgttctt
    44881 gtctgtgcac gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggcgaat
    44941 ttggcctcgg cgtggcggta gatgtctacc atgtctggtg ggacccggat ctcgccagtc
    45001 agatcctgcg tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttagtgccga
    45061 ccaccgatct ggtcaatgac cgtggaatgc cgggagatgg tgtcatcaat attccgtcaa
    45121 ttcgtcggct ggttgaaaac gccggattta atggcgctat tgaactggaa atattctcac
    45181 cgtactggtg gcagaaagat attaacagca cgctggatat tagcgtcgat cgcatcgcgc
    45241 attattgtta aggaatgatc atgaatgacc aacccgttct gttcagcaga gcggcggctt
    45301 cttgccgcct gacattgaac cgtgaagata aatgtcatgc tattaatgaa gaaatgatcg
    45361 aatctctcga ccattattta aatgaaattg aaaatgacac ggcattacgc ttagtcgaat
    45421 taacggcaac cggcgataaa ttcttttgtg cgggtggcga tattaaatcg tggtcggcct
    45481 attcgccatt agatatgggc agaaaatgga ttaaacgcgg taatgacgtt tttaatcgcc
    45541 tgcgaaactt accccaatta accgttgcga atctcaacgg tcataccatc ggcggtggta
    45601 ttgaacttgc cttgtgctgc gatattcgta tcgctcgccc cggcgcgaaa ttctctaacc
    45661 cggaagtgat gctcggcatg gtgccaggct ggatgggcat tgagcgagtg cttaatcagg
    45721 ttggtcctgt ggtcggtcgc cagatgctga tgctgggtaa acgcctgacg gcgcaggaag
    45781 ctcagacagc gaatttaatt gatgaagtgg tagaaaaaga acaggtggaa agctggatgg
    45841 cgaaccagtt agcgcagctg gaaaaatgtg gtccggtggc actggcacat attaagcaac
    45901 tgattctggc gctggaaaat aaacacgccg attactcgca ccagttgctg gctggattaa
    45961 tgtccgccac tcaggattgc caacaggcga cgcacgcatt tgccgaaaaa gccggcgtga
    46021 gcttccacaa tcaataagga aattgtatgc gtaagttatc aaccgccgag acactggcgg
    46081 cgcaaattca ggacggcgca accattgcca ttagcggcaa cggcggcggc atggtggaag
    46141 ctgaccatat tctggccgcc attgaagcgc gctttctgca aaccggacac ccgcgcgatc
    46201 tgacattaat ccactcgctg ggtattggcg atcgcgacag caaaggcact aaccgttttg
    46261 ctcacgccga aatgctcaaa cgcattattg ccggacattt tacctggtcg cccaagatgc
    46321 aggaactggt gaaaaacaat gctattgaag cctactgttt ccctggtggc gtaattcagg
    46381 cactgctgcg ggagatcggt gccggacgtc cggggctttt tacccacgtt gggctgggat
    46441 cgtttgttga tccacgcaat ggtggcggta agtcgaatga atgcactacc gacgacctgg
    46501 tagaactgat tgaaatcgac ggtgagacca aacttcgtta tcgccctttc aaagtggatt
    46561 acgcgatttt gcgtggcact tatgccgatc cacgaggcaa cgttagcctc gaagaagaag
    46621 cgattgatat ggatagctat tcgatggcgc tggcagcaca caacagcggc ggcaaagtgt
    46681 tcgtacaggt acgcgatgtg ctggaagcag gtaccattga accacgtcgg gtcaagctac
    46741 caggaattct ggttgatggc atcgttgagc accgcgaaca accgcaaacc tatcttggtg
    46801 gttacgacct gaccatcagc ggtcaacatc gccgtctaag ttctaacgac gctattgaac
    46861 tggttagtca tccggtgcgt cgcctgattg cccgtcgggc agcgcgggaa ctggtggcag
    46921 gcgcttcaac caactttggc tttggtattc ccggcggtat tcctggggtt gcgctgcgcg
    46981 aaggcgtgcc ttatcaaagt ttgtggctga gtgtagaaca gggtgtgcat aacggcatga
    47041 tgcttgatga tgctctgttc ggctgcgccc gtaacgccga tgccattatt ccatcactcg
    47101 atcaatttga attctacagt ggcggcggca tcgatatcac cttcctcggc atgggagaga
    47161 tggatcaata cggtaacgtc aacgtatccc atctcaatgg caatctgatt ggccccggcg
    47221 gatttctcga aattgcacaa aacgcccgta aagtggtgtt ttgcggtacg ttcgacgcca
    47281 aaggcagcaa gattgatgta acgccagatg gcttgcatat cgcccagtca ggtcaaatcc
    47341 ctaaactggt tacccaggtg gaaaaaatta cttttagcgc cgcctacgca cagcaaagtg
    47401 gtcaggaagt gttgtatatc actgaacgcg cagtattcca gttaacggca gaaggcgttg
    47461 aattaattga aatcgcgccg ggtgtggaga ttgagcgcga cattctgccg tatatggcct
    47521 tccgtccgat tatcaggcac ccacgtctga tggaaagtag cctgtttatg ccaatggagg
    47581 atgcatgagt cagcttgatg acaccatcct tgatgcactg acgcacgtta ctttcccaaa
    47641 gggttttgca caggcagaac ccgcatgggt tgtcacggtt gacggcgttg attacccact
    47701 ctggaaaaca gatgccctgg tagtcggcag tggtgcagcc gggctacgtg cagctgtgga
    47761 acttaaacgc cgccagcaaa atgtgttaat cgccaccgcc gggttatata tggggacgtc
    47821 ggcatgttct ggctctgata aacaaacact gtttaccgcc gctactgcgg gcaacggcga
    47881 caacttcacc aaactggcag aggcactggc gagcggtggg gcgatggatc acgacaccgc
    47941 ttatgttgaa gcggtaggtt ctctgcacac tcttggcggg ttgcaatatc ttggtctgga
    48001 attaccggaa gatcgctatg gcgcgattct tcgttatcaa accgaccatg acgaagccgg
    48061 gcgtgcaacc tcgtgtgggc cgcgtacctc aaggttaatg gtgaaagtgc tgctggaaga
    48121 agtacagcgt cttgctatcc caatgttgac cagtgcaaca gtgattaaac tgctgcatca
    48181 gcgtgacgaa aacggtgaag accgtgtggc gggggcaatc ctcgcgaccg gtcatcgcgc
    48241 ccataaccca tgggggctgg caattgtgac tgcgcccaat gtggtactgg caacaggagg
    48301 gcctggcgag ctttatcgcg acagtgttta tccacacaaa tgtttcggct cgctgggtct
    48361 ggcgctggag gaaggtctga cgctaaccaa tctgactgaa agccagtttg gtatcggcac
    48421 accgcgcagc acgtttccgt ggaatttgtc cggcacctat gtgcaggtga tcccgtatat
    48481 ctattccgta gatgctgagg gtaacgagta taacttcctc gcggattact atcgcaccac
    48541 ccaggagcta gcttcaaaca tcttccgtaa aggctatcag tggccgttcc acgccacgcg
    48601 ggtaatggat tttggctcca gcttgttaga tatggcagta gcgcaagagc agcaatcagg
    48661 gcgtcaggta tttatggatt tcaatcgcaa tcctgaagcg gtgccgggtg acctgccctt
    48721 ctcattagat cgactggacg acgacgttcg cacgtatctg gaaaataacg acgctctggc
    48781 accatcgccc atcgaacgcc tgcaacgaat gaatccgctg tctatctcgc tgtataagat
    48841 gcacggttac gatctcacca cgcagccatt gcagtttgcc atgaataatc agcacatgaa
    48901 tggcggcatt gaagtcgata tctggggaca aacatctctg cccggttgtt ttgcggtggg
    48961 ggaagttgct ggcacacacg gcgtcacccg acctggcggc gcggcattga atgccgggca
    49021 ggtttttgct gttcgtctgg cacgttttat tggttgcacg caaaagcgga atattgatgg
    49081 agatatagca cagctggcag ctccggcact ggcttctata agagagatca ttactcaggc
    49141 gcacgataac gggaccggga tgccgttgtc ggttgtgaga gaaaaaattc aggcacgaat
    49201 gtctgaccat gcgggattta tttgccatgc cgataaagtc cgacgcgcca ctcgtgatgc
    49261 cctgctattg aacgaatttg tccaacgaca tggattggca atcaaacatg tgggtgaagt
    49321 tgccgagctg tttatgtggc ggcatatgac gctgacttct gccgcggtat taacacaatt
    49381 aacgcattat attgatgctg gtggtggtag ccgtggggcg cgtatggtta ttgatccaca
    49441 aggcaaatgc ctaccacaaa ctcgtcgcgg cgcaaaagaa gaatggcgtt ttcgctctga
    49501 actcgctgaa gacaaaaatc acaaattaac aattcaatat tcgcaaggtt cttttattac
    49561 cgaagtaaag tctttacgta tgcaaccgtg tattaacggt atttactttg aaaaaaactg
    49621 gccagacttt ttaaagggag acatttacac acaataattt ccaatatttc ctctgtacct
    49681 gatttttatt gcctattaca tggaaatacc aacaaccagg gtttattaat ataattaaaa
    49741 ggtttaaaaa tgaataaaat aaagaattat cgatggcata tgattgccct cgtatgcttt
    49801 atcactgtaa tcaattatct ggacagaacg gcgttaggta ttgcggctcc aacgattatg
    49861 gagacaaccg gaataaccaa agaacaatat tcatggattg tcagtgcatt ccagttggcc
    49921 tatacattgg ggcaaccgat aatgggcttc tttattgata ccgtgggtct gaagttaagt
    49981 tttgcgatat gtgccgcaat ctggggcctg gcgacaatgg ggcatgcctt gacaggaaca
    50041 tggtcaggtc tggcatttat gcgcgccctg atgggtttca gcgaagcatc ggcaattcct
    50101 gcgggtgtaa aaaccgcatc aacatggttc ccggcaaaag agcgtggcgt ggcgacaggt
    50161 gttttcaata tgggcacctc actcggcgcg atgcttgctc caccgttgat tgcctggtgc
    50221 attatgtttc atagctggca atttgcgttt attgtctcag gtagccttgc tttactcgcg
    50281 gctttatttt ggttcttttg ttataaagat ccaaaagatg ctaaacgcct ttctgatgaa
    50341 gagcgccact atattgagtc tgggcaagaa cagcatctta aaacagataa gaaagaaaaa
    50401 acgtcaatca agcatatcct cagccaacgt aatttctggg ggattggcat cgcgcgtttt
    50461 ctcgctgacc cggcatgggg aaccattaac ttctgggtac cgattttctt cgtcgaaacg
    50521 ctgcatttta gcctgaaaga aattgccatg ttcgtctggc tacctttcct gctgggcgat
    50581 ctcggctgtt tagccagtgg ttttgtcgcg aagttcttcc acgatcgcgg cgtgagttta
    50641 attaactcac gaagaattac cttcactatt gcagccgtca ttatgatgac gattggtctg
    50701 gtgagtattg tcgaaaatcc ctacatcgcc gtattactga ttagcattgg cgcattctcg
    50761 catcaatgtc tttctactgt tgcagcaact ctgggcggtg acctattcaa aaaagacgaa
    50821 gtagctaccg ctgtgggtat ggcaggagcc tgtgcatgga gcggtcagtt gattttcaac
    50881 ctgtttatcg gggcattcgt tcacattatc ggcttcgcgc cgttctttat tgccctggct
    50941 ttctttgaca ttattggcgc cattgcgctg tggacgctta tcaaagttaa agacgaagaa
    51001 ccacaagtac agttagcgac aagctaatta ttttacatag aaacgctata atttagcttt
    51061 gtagcgtttc tatttttctc ataaattaaa cgtctcttcc aaaacgttca gtatctactg
    51121 gcattgcttc gcaaatttat gatttagcga atatatcgaa ataactattc taaattgccc
    51181 ccctgaacgc ctctgccatt aactcttgct cacgaccagg taatcgaaga cgctcggcaa
    51241 tgattcgcca ctgactgaca atccctctaa aattagcaat aatgtcatca gcttccgtca
    51301 aactaagtcg aaaatactct gcaacttctc tagctaattc caaatcaagt gcattgtcat
    51361 tttccgtaat attgagtttt agaccatcag acctgaccac aggattcaaa tcatatgcct
    51421 cagaaagccg ccaacctttt cctggtatca agataaatcc atgattgcgt aaatgatcat
    51481 cggtattagt aacaagaata ttaaaaacaa tcctggacca gagttcctta aggtcagtat
    51541 tagtctgcgc gccatgtctg ataagaacat ctgctaattc cagataactc acacctgtag
    51601 aggcatcttc accatcctga tgcctggtta gtgtcatagc cgaagcaaaa tgcagacgcc
    51661 ttcccgcatt agtgcggtca aaacgacgaa ccataaaaca atggtaattg ctggcaaatt
    51721 tatgcgcctg agcaggtgca acattcagtc cacagccaac agcaagtgca ttaacgacca
    51781 tttcccatcc accaacatca tactcatctt ttacactggg aaatttagcg atgtagagat
    51841 gcccagcttc atcaacaaca ctggctttcg gacgtgcccc ccctaatgat ccccccggag
    51901 cgattagcat tcttaaccat ttttgcccca ttactcttta ttatcaggat cttcttcaat
    51961 tgcccggcta accctttcca gactcgctat ttcggtaaat ggtggggctg caacatcaat
    52021 gcgattatca agaaactctc cagcgtcttc acgtttgtat cgaagagccc caacccgata
    52081 aagatcatga acacctagta gataatcaga ctcataaagg tgggagtctt tatcacataa
    52141 cccgtctcga atatccctct caaaacgacg cttcattagc atacgccccc aacgatccgg
    52201 gcaggaatca ctaaatgcac caaatttatc ctttggagga attgggtact gtgctccctc
    52261 atacaacatt atttcaggat ctaactgaat gaaattaagt gaaggatctg caagcgcctt
    52321 tttatcgtac tcaaactcaa atatctcacg cgcccgagtg cgacgactgt gtaaataacc
    52381 aatacgcgca ggaccatcca ggccttccca atccgcatac actccgatta atgccataat
    52441 taatttcctt ttaacggttt ttttatcggt tttaatagtg cagcaagaga ggttgatgtt
    52501 tctcctgtaa tagatggcgt cgagccttct gccagtgctg ttaaatccgt ggtgagatga
    52561 ccttctttct cgtgcctcat ctgcaatgag gctgtctgag aaccttcttc attcttactt
    52621 gatacacgag acagccctct ttttttaatc aaacgggaat cctggagctg acgccctaag
    52681 tcgtcttcag ctgcaagctt atttaaatcc gtttccaggc ccaaaacctg catcacagac
    52741 aaatatgccc ctatcgtgac ccctgcactt cctgactcca gagagcgcaa tgttattggc
    52801 gacatcccag cacgttcagc aacttgcttt gcggtaagcc tgcgtcgtaa acgagcaagt
    52861 ttcagccgct caccaaattc actaagcatc tgattcgtat aagggagtaa cggtgctgtt
    52921 tttttaccca tagtgataat attttatctt tttatggcta tgatagccat aatattagca
    52981 tttattggat tgaacgcacc tgcaaagcgt tcaagattac gaaagtgatg atattttatc
    53041 tttttatata taaaaacaat aaaataacag cacattcata tataacatat tgtttttatt
    53101 atcatacaag tgagacgatc gctgtattga tgcttttgtc gtgttttacg cgtaaaaaac
    53161 atgacacgaa ctaccagcgg cgaggctggg ggccaggtat gggtactgcg aactttttat
    53221 aaaaggaata gcaagagatt tatggaggga acgtctgttt ctgcggtttt tatcacttaa
    53281 aagcaaaacg ccgaccactg gtcggcgttt ttacgtctcg ttgaataacg aattattcag
    53341 ctactacgtt tacgatcact ttcgcgaata cttcgctgtg aacctggaag ctcacttcgt
    53401 gttcaccagt ggtacgcaga acgccgttcg gcagacgaac ttcgctctta gccacttcaa
    53461 cgccagctgc agttacagcg tcagcgatgt cgcgagtacc gatggaaccg aacagtttac
    53521 cttcgtcgcc agctttagac gcgatggtaa cagtttccag tgcattgatt ttctcagcgc
    53581 gagcattagc tgctgccaga acttcagcca gtttagcttc cagttcagcg cgacgtgctt
    53641 cgaagaattc aatgtttttc ttggtagctg gaacagcttt accctgcggt accaggaagt
    53701 tacgagcata gcccgcttta acgtttacct gatcacccag gctacccagg tttgctactt
    53761 tatcaagcag aataacttgc attaccttat cctctcaaag tcgtattaat ggaccgtgac
    53821 cgattactga tggcgatcag tgtacggcag cagggacagg tagcgagcgc gtttgatagc
    53881 gcgagccagc tgacgctggt attttgcacg ggtaccggtg atacggcttg ggacaatctt
    53941 accgctttcg gtgatgtagt ttttcagcgt agcgatatct ttatagtcga tctcttgaac
    54001 gccttccgcg gtgaaacggc agaacttgcg acgacggaaa taacgtgcca tatggctagt
    54061 ctccagaatc tatcaattca atctgctcgg catgcaaaac cattttgctc agtccgttct
    54121 ttgccttatg gcatgaaatg aacccctgaa cggttatgcg actgccgacc gttatactgt
    54181 gagtaatggc ctggttttcg tgtccgctaa caataacggg catttggcac cacgcctgcc
    54241 ggtgaaagcc ggcttcctcc tgcacagaac gatgctcaag cacgaactgg cagtgaggaa
    54301 ttcctgatgg actgaccttt cgaaggggag tcctgcacac ggtgccggac aacaccagac
    54361 ggttggtcat cagaaattac tcttcagaat ccccagcttc agcatcatca gcggtttcgt
    54421 ttgcgaaatc atcgcgacgc tcacggcgct cgtctttcgc tttaaccatc ggagatgctt
    54481 cggtaacagc gtgcttggta cgcataacca tgctgcggat aacggcatcg ttgaagcgga
    54541 aggtagtttc cagctcatcg atcacttcct gcggagcttc aacgttcatc agaacgtagt
    54601 gtgctttgtg cagtttgttg atcgggtaag ccagctgacg gcggccccag tcttccagac
    54661 ggtggatctt gccttctgca ccagtgatgg cagcagtgta gcgctcgatc atgcccggaa
    54721 cctgttcgct ctggtcagga tggaccataa aaacgatttc gtaatgacgc atcgaattgc
    54781 tccttacgga ttattcagcc tcctgtctgg gtcagccgaa tcccggggag gcaaggaacg
    54841 tgttaaaggt cggctgaaaa atgacgcgtt atagtactga cataccccgc taaactcaag
    54901 ggctgagcag aaataaatcg cccctttcgc aaataacctc taaatcgctg atttggagtt
    54961 catcacactt cgtcatggtg tttttttgaa caactgcatc agaaatggtc accatcaact
    55021 tctttatcat tgattaaaat taaatcagaa gatcaccaca agaggagagc acacaaagtc
    55081 agccgtcgag gaggtatcta tgttcagtcg tgttttagcc cttctggctg tgcttttgct
    55141 aagtgcaaat acatgggcag ccattgaaat taataaccac caggccagaa atatggatga
    55201 tgtgcaaagc ttaggtgtga tttatatcaa ccataacttc gccactgaaa gtgaagcacg
    55261 tcaggcatta aatgaagaga cagatgcgca aggcgcaacg tactaccatg tgattctgat
    55321 gcgggaaccg gggagtaacg gcaatatgca cgccagcgcg gatatttatc gctagcacca
    55381 ggtataacca acgaaacatt gccatagttt gctttgcccc cttcgcaggg ggcttttttt
    55441 gaaaaacggc tgcggaatta gaccagttat ctcccgaaga aggaaatttc cgcagcgcgt
    55501 gttttacttc tgcccgtaat aggcgttggg accgtgctta cgcatgaagt gtttattcat
    55561 cagaaagctg tcgatgtgat tcagttgtgg gttaatactg cgcgcaatcc acgccatttt
    55621 cgccacttct tccatcacca ccgcgttatg caccgcatcg tgggcatctt tcccccagac
    55681 gaacggtccg tgctgataca ccacaattcc cggcgtatgc agcggctcgg cgtcacccag
    55741 cgtttcgata atcactttgc cggtattcag ttcgtactca ccctgcacct cttcttcgct
    55801 taatccgcgc gtgcacggga tgtcgccaaa gaagtaatcg gcgtgcgtgg tgcctaacgc
    55861 cgggatcgcc agccccgcct gcgcccatgc ggtggcatga gtggagtggg tatggacaat
    55921 accaccaagc gacggataac gacggtagag ttcgagatgc gtcgcagtgt cggaagacgg
    55981 acgatactcc ccttccacca ccttaccgct catatcgacc accaccatat cgtctgcttt
    56041 catggtttcg taggcgacgc cgctgggctt gatcaccacc agcccgcgtt cgcggtcaat
    56101 ggcgctgacg ttgccccagg taaaggtcac cagcccgtaa cgcggcagat ccatgttggc
    56161 ctcaaacacc tgctgtttca gcttttgcat tataccgcct ccaccatgcc tgctttggtc
    56221 atgcgcgctt tcacccaatc gcgcgctttc gccacttctg ccgccggatc ttccgccgtt
    56281 tcgctccaca tctcaatcag gtacggcccg caatagccac tctgtttgag cgtttcgaag
    56341 caacgttcaa aatccacaac accttcgcca aaaggcacgt ttttgaagac gcctggtttg
    56401 gtgtctttca catgtaccgc gacgatatgc ccgattccgg cctgcaactc catctgcacg
    56461 tcgttgtccc acgccgacag gttgccgata tccgggtaga gctggaacca cggattgttg
    56521 agatagtgtg cgtagcccag cgccttgctg atggagttca tcaatgggta atccatgatc
    56581 tccatcgcca gcgtgacctg cgcgcggctt gccatctcaa cgctctcttt caggccgtca
    56641 cgaaaacgac gacgcgtttc gttattggct tcctgatagt aaacgtcata gcccgccagt
    56701 tggatcacgc gaatgccgac gtcctgggcg aactggatag ctttacgcat aatctccagc
    56761 ccctgcgccc gcaccgcgtc atcttcgctt cccagcggga aacgacgatg agcagaaagg
    56821 cacatggacg gtacgcgcac gccggtttca acaatcgcgt tcaccagtgc cagacgctgc
    56881 tcgcggctcc agtcgaggcg cgaaaggcga tcgtccgttt catctactga catctcgaca
    56941 aaatcgaagc ctaacgtttt tgccagttgc agtcgttcca gccagcactc cccagcgggg
    57001 agcgcttttt cgtagatgcc aagcgggatt tgtttggaca acatatccgc tccttagccc
    57061 cacagttcgg cgatggaacg tttgaactga cgcgcggctt caaccggaga ggctgcatca
    57121 cggatgctgc gaccagcgat aaacacatga atcggaatgc ctttaaacaa cggcagatct
    57181 tccagcgcca gaccaccggt gacggtgact ttgaagccca tatcggaaag acgtttgatc
    57241 gcggtaatgt ccgcttcgcc ccacgccacg cctgcggcct gcgcgtcacg gctgcggtga
    57301 taaaccacct gcccaatgcc tgcgtcgcgc cactgctgcg cctgttccca ggtccagtag
    57361 ccggtcagtt cgatctgcac gtcgccatta aactcttttg ccacgtccag cgcgcctttg
    57421 gcggtgttga tatccgcaca acaaattacg gtcacccagt cagcgttggc ttcgaagcac
    57481 atgcgagaaa ggattttgcc tgcatcggca attttggcgt cagccagtac gattttgtgc
    57541 gggtacagcg ctttcaggtc acgaaccgca cgcacgcctt cgcctacgca aagaatggtg
    57601 cccacttcga taatgtcgac ttcttcggca atcaggcgag tggtttcgta ggcgctatcc
    57661 atagtctggt tgtccagcgc gacttgcaac atcggtaatg acatgttcaa ttccttctta
    57721 agctgccgcg ttggtgcggt caatgagatc cagtacttcc tgctcggtac ggcaggcacg
    57781 taaacggtcg aaattctctt catcttcaaa caggttgacg atctgcatga tgcccacttc
    57841 ctggtgagtg ttggcatcaa ccgccgccat ggtgatgagg atatccaccg gatcgttatc
    57901 ttcgtggttg aactccagcg gctttttcag cgtcaccagc gcaaaaccgg ttttcttaac
    57961 gccctcttcc gggcgtccgt gcggcattgc caggccgggg gcgagcacaa agtacgggcc
    58021 gtgctgttca accgcatcca gaatcgcctg gtagtaacgc ggctctacca catctgccgc
    58081 taccagcaga tcaacgccga ttttcactgc gtcctgccat gtctctgctt ccgcttgcag
    58141 gcggatagat ttattttcag ccagcgaatc acgtaatttc atggcgcgtc cttacttcac
    58201 gtcctgcggg aaatgctctt tgatcacttc cagcagtttc gggccaaaat cagcaggaga
    58261 gagcatgttg cgcacgccaa ccacatattt gttgccggtc acggtgattt cgcccgcaat
    58321 atgggtagaa gcgatgatga tatccgcgcc gctcaactcg cttttgtatt cgccaaccgc
    58381 gcagctgttt accgtgtggt caatgttcga ctgggttaaa aactggtcca ctttcatttt
    58441 catgatcatg gaactgcctt gtccgttacc acacacagcc agaatacgaa cggtcataat
    58501 caaaactcct tattaagcag attgttctgc cagttgtttt tctgcatctt cttctgcgcg
    58561 cagcgcgcgg ctagcgaaga acatataagc cagtgcaatt acaatgatga cggccataaa
    58621 ggcgataccg atggagaaga agccctgcat catcggcggt gccagaatcg accagtccgc
    58681 catacccatc caggcactca taccggtgag tttcactgcc cagacgcaac caaagatttc
    58741 gatcatcccc atcaccagac aaattttcag cgccgcacgc cagccgccga agtggttagc
    58801 gaacacgccg atagtggcgt tggagaagaa catcgggata aagccgggaa taatcaggat
    58861 tgaagagcca caggcgacca ggataccaac cgcaatcagt tgcccgatgg tgccccacat
    58921 aaaaccccag accacggcgt tcggcgcgaa gctataaata gccgcacagt caatcgccag
    58981 aaccgcacct gggatcagac gctgggagat accgttaaat gcttcagaga gttccgccac
    59041 aaacatgcgc acgccctgag tgatgatgaa gatcgccacc gcaaaggaga aaccggtttg
    59101 caggatgtac accgtccagt tcactttgcc tgccatcgcc tgcacggtgt cgataccaaa
    59161 ggagagcaga atggcaccaa agaagatagt catgacaatc gccgtggaga cgatgttgtc
    59221 gtggaaaata ttcagccagc caggcagttt gaggtcttca acgctttcct cttttttgcc
    59281 aaggaacggt gcgactttat aagcaatcca tgatgcgaac tgctgctggt gaccaatgga
    59341 gaaaccacag ccgtcggtga cttcctgtgt cggcttgtac atcatgttgg aggtaatgcc
    59401 ccagtagagc gaaaccagaa tcgcggtgca gataatggtc gtccacatgg agtagccgaa
    59461 gataaacagc gtaacggcaa tcagccccgc ctgctggaac atgatgtgac cagtcagcat
    59521 gatggttcga ataccggtaa tgcgacgcag cagcacgtaa cagatgttca gcgccagcgc
    59581 taacaacacc gcgtaaccca cccagctata ggcatcgccc atgcgttcaa tggttgccat
    59641 catcgacgcg taagtatcgg aaattgcgcc gttaatgccg tagacttcgg acattttcgc
    59701 caccaccggt ttgaaggtgc tggtcaggat gccggaccct gcctgcaaca acatgaaacc
    59761 aattatggtt ttaatcgtac ctttaataat aacgctgaca cttttgcgca gtaggatgta
    59821 acccagacag gtcacaatac ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa
    59881 cacggtaaag atgttgtaga ggatctccat aacgatctcc tgaagaagag gtaaccgggt
    59941 atttcgcaca cccggaatgt tgtgctctca ctctaatttt caaaagtaat cacaacaaga
    60001 ttatttatga ttaaatgtga cgtcgcacgc aaataattcc acataaacag aatgggattc
    60061 acggaatgaa atatttattc atttaaattc atttagttaa ttgcattaca cattcaattt
    60121 aatctttcta cactcaaaat ccacaataac tctcctgcta aaaagtcaaa aaatggtcat
    60181 tgccttcctg aaaaaatagt gtcaggatta atcaaaacca atcacatatt gattcgaatt
    60241 gaactatcac gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg tgagtcctgg
    60301 atcctgagca ctttcccgga gtggggtagc tggctgaatg aagaaattga gcaagagcag
    60361 gtcgcaccag gcactttcgc aatgtggtgg ctcggctgta ccggaatctg gttgaaatca
    60421 gaaggcggtg ccaacgtttg tgttgatttt tggtgcggta ctggcaaaca aagccacggt
    60481 aacccgttaa tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag
    60541 ccaaacctgc gcaccacccc gtttgttctt gatccgtttg cgattcgcca gatcgacgcg
    60601 gtactggcga ctcacgatca caacgatcat atcgacgtta acgtcgctgc tgccgtgatg
    60661 cagaactgtg ctgatgacgt accgttcatt ggaccgaaaa cctgcgttga tttgtggatt
    60721 ggctggggcg taccgaaaga gcgttgcatc gtcgttaaac cgggcgacgt agtgaaggta
    60781 aaagacattg aaattcatgc acttgatgct ttcgaccgca cggcactgat cacgctgcca
    60841 gccgatcaaa aagcggctgg cgtactgccg gacggcatgg acgatcgcgc ggtaaactac
    60901 ctgttcaaaa cgccgggcgg ttccctgtat cacagcggcg attcccacta ctccaactat
    60961 tatgcaaaac atggtaacga gcatcagatc gacgtggctt taggatcgta cggagagaac
    61021 ccacgtggta tcaccgacaa aatgaccagt gctgatatcc tgcgtatggg cgaagcgctg
    61081 aatgcgaaag tagtgatccc gttccaccac gatatctggt caaacttcca ggccgatccg
    61141 caagagatcc gcgtgctgtg ggagatgaaa aaagatcgcc tgaagtatgg cttcaagccg
    61201 tttatctggc aggtcggcgg caaatttacc tggccgctgg ataaagacaa cttcgagtac
    61261 cactatccgc gtggtttcga tgattgcttc actattgaac cagatctgcc gttcaagtcg
    61321 ttcctgtaat ctgtcgcttg taccggatag cgtggagatg ctcgcgctat ccggcatagt
    61381 atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa tagctcatga
    61441 ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc tttgtgaccg
    61501 ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcacgacgc gatatcaata
    61561 aacttgacga aagcggcaaa ctgaaaaaag tgcgcaatgg cgcagaagct attacccaac
    61621 agcgcccgcg ctggacgccg atgaatctgc atcaagcgca gaatcacgat gaaaaagtac
    61681 gtattgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc atcaactgcg
    61741 gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta
    61801 attatctacc gctggcaaat tacctgatcg atcaggagca tgacagcgtg atcattatgg
    61861 gcggacagta caacaaaagt cagtccatca ctttaagccc gcaaggcagc gaaaacagtc
    61921 tctatgccgg gcactggatg tttaccagcg gcaaaggact gacagcagaa gggttgtata
    61981 aaaccgatat gctgacggca atggcagagc agaagatgct gagcgtggta gggaaactgg
    62041 tggtactggt tgatagcagt aagattggcg aacgcgcggg aatgcttttt agccgtgccg
    62101 atcaaatcga tatgctgatc accggcaaaa atgctaaccc ggaaatcctg caacaactgg
    62161 aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag
    62221 catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca
    62281 gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca
    62341 cgacgtcatc gtcaaggcca tgccataaga gcagcggtct gttccccaac tctgtcagat
    62401 ggtttgtcgc ttcccactct gccagcggcg cgacaatgtt attgaattca ttctgctgtg
    62461 ctgccgtttc aggtatcagc ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg
    62521 atcccatcat gctggcggta cattttaccg tggggtggcg agcggtaatt cccagtgccg
    62581 tcatcgcgcc catcgacgcg ccaccgactg ccagacggtc atcacgcagc cagttttctt
    62641 cggctattgc cgcacgtaag gtagtgaatt cctgcatact ttgtagcaag atttgccaga
    62701 attgatttaa ccgccgcgct gcgtcgccac taaaacggct accgtgatcg ggcgcatccg
    62761 gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccagac
    62821 tggacgaagt aaagccgtga taaaaaatca cacacggtaa cggggtatct ttttgcccga
    62881 caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct
    62941 gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg
    63001 tattagttca taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg
    63061 cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta
    63121 tggttagcag gaaacgtaat agcattattt accggtttgc cagtttatta ttggtgttga
    63181 tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacggacc
    63241 atccgcaaga gattcgccgt gaccagacgc aagggttaca gcgaataggt agcgtaagca
    63301 cgatggttcg aggttctccg gatgacgcat tagcagaaat cagagcgaaa gctgtcgctg
    63361 caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt
    63421 attcacaagc cattttgtac cgtaaataaa tcagacaact aacatagtca gctttacatt
    63481 gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa gctcgggcgt
    63541 ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg
    63601 aaacaattac ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca
    63661 cagtctgcag aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc
    63721 tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc
    63781 gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat
    63841 aactggcgcg tacaggcgat actgtatgcg tgagtgtcat ctacagcaaa tgaacaatgc
    63901 cggatgcggc gcgagcgcct tgtccggcct acaattttaa aatatttcat ttagttcatc
    63961 gaaaccagcg tcgcatcagt cgtatacgct tacaaacaca ctcctcccgt cgcccgcagc
    64021 aataaatcat tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc
    64081 ttacaccacg tctgcgccat tggcggagag gcatatttca acatttgcgc accgcagccc
    64141 agcaggaata gctgatgagt aatctctcgc cccaattctt cagctggctt acgcagctgc
    64201 tgctgtaaac gacgaaccgc gcgatcaaaa tagcgatcct gccctttcac ttccacaaat
    64261 gcttccgaca ataagtcgta tacgcccgct tgcttattga gaacgcgcag cacatccagg
    64321 cacataatat tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc
    64381 ggcagctcgc tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca
    64441 aacggaatgc cacgtttgca gatcacaaat ttcgccgcag gcgtaaataa acgcgcccac
    64501 agagcttctt tggcatcggc gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc
    64561 gtttgccctt caagctgaag tgccatgcga cttaagacat gacgcataag gggctgttgg
    64621 atcaatggat taccaaaaac atggcgttga tgtgcatgat aaatcgccag cgaaaatgca
    64681 cggcgcatca tggcatggct acccagggcg caatcaaaac gcgtcatccc acccattttc
    64741 aggatcagac gaattccttc cccttcctgc cccaacaacc agccaatggc atcctgaaat
    64801 tccacttcgc agctggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga
    64861 atcgcgttgc gttgcccgtc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc
    64921 gcggtctgtg ccagcaccag atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc
    64981 cccaccagcc gataagaacc atcgtccaga cgctctgcgc gggtggtgtt gctcatgaca
    65041 tcggaaccgc cctgcttttc cgtcattccc atgccgatca acaagccacg tttttgccca
    65101 cctggcaata agtgagaatc gtagcgatcg ctcaacagcg gcgtggtcca gtcctgaaac
    65161 ggcgcgggta acatctgtaa caacaatggc gtggcggcaa aggtcatcgt tatcggacat
    65221 aacgacccag cctcaacctg cgcatgtaac ataaaacgcg ccgcgcgcgc cacaaatgcg
    65281 ccggagcgag cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc
    65341 atcagcaggt gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg
    65401 tagcgcaata attcaggcgg attcacattc gccagccgcc ccagttcaag ggattcagcc
    65461 gttcctaact gctgaccaat actggcgaga aaatcgctat cccagccagc accttcacgc
    65521 gttaccgctt cgcaaagcgc gccatcagac aggtataagt tgctgttatt taatggtata
    65581 ggttgattaa aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt
    65641 caccattagt atggtcactg ccatggattc atgacagaaa gagattgcta aaacattctt
    65701 atccagcaat gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagc gtgttatctt
    65761 cacggatccc cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc
    65821 tatcgccaaa tcgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat
    65881 caccatcagc gtggtcaaca acgttattct gaccgtcgaa aatggtgacg tttccgcctt
    65941 cgcgcgagta aatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcac
    66001 catcgaacca ggatgcaaga aggttaggat gaccagggaa gaagcgccaa agcagcggca
    66061 ttagcccttt attactcaag atacttttcc ataacggctc cacccattgc tcacgacgct
    66121 tgcgcagcag cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg
    66181 cacgctggat gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagct
    66241 cttcaatgta gataaaccga gactcctgcc ctgcctgctc ggcgcagtcc tgcaagtaca
    66301 gcacggtact cctgtcttca tcggtatcct gacagcagca aaaataaaac ggctcccggc
    66361 tataaagctc gctaaagcgt gaaatcaggc gctcctgaat ggcattgtac tgatcggcat
    66421 cgcgcggaat aacgccgctg cgccgggcat cttccagcca caaccactgg aaataagccg
    66481 actcgtacaa tgaagttggc gtatcggcgt tgtactccag caacttaacc ggtgcattac
    66541 cacaccaggc aaaatccata cggccataca acgaaggatc gcgtgcgcgc cagctttcgg
    66601 cgatcacatc ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcatctttaa
    66661 ccgcacgatc caccacctcc aggcacatct gatgcagttc cgcagtcggt ttttcgatct
    66721 gctcttcaat ctggcgcaga gtaaagcggt aagcccgact ctcatcccaa tagatttcat
    66781 tgtcgatgat atgaaagtca aatccgttat cggcggcaat ccggtccaga tcccgccgca
    66841 caggaacgtt gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg
    66901 accatagccg ccgcgagaaa cggttgacgc ttttttggtg gtaaagccgc ccgaagagta
    66961 agactctttt ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgcgccagac
    67021 ggggcgggaa gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt
    67081 acgaataacc cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt
    67141 gtcgtagtaa caattttcat acttagactg acaattctgc tgagtcatat tcttcggaac
    67201 atcggcataa aatgccgctt tggcgttgtt ccagccatga gcacagatat ctgaattatt
    67261 accatcatca atacaatcct gcacagtcgc gtaaaacgtt ccgtcgccgt cgttatcgac
    67321 atcgccacta tcgccacaac ctttcaatac gaaaaaagcg gcaccgccca ttagcgccaa
    67381 cgttaagtat ttttgtgcgt agcgattgcg gcgcggctca aaaggattat gcggtctacc
    67441 aatgcgactg attgccccat gtccgatatt tttgttattt ctggatttgc gttttctggc
    67501 catgatcgtc cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc
    67561 ggttcccata aacattccgg ctgcggtatt gtgattaata attttttcgc ttaatgcggg
    67621 catataaagc ctgacgccag caaaaacgag taattgaatc accagagcaa tcccgcccca
    67681 tgcgaaatag tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta
    67741 ccccaacaat gttccgctga acgccagtgc cgccgcggtg ttattgtttt tgattaactg
    67801 ccattcgttg tgcggtgtaa ttttggagta gataaacagg aaaataatca ccatcgccac
    67861 gccaataaaa aaataggcac taaaagcaag aagtgaatcc agtatatgca ttgtaaacgt
    67921 ccttgtaatt aaccaataat atgaagtgat gtcagcggga tatcgatacc cagcgcgcgc
    67981 gaaaataacc actccggctc gccgagatcg ttaaaagatt cctcaccatt taacagcaaa
    68041 tattcgtaag tatctccggt cacctgccgt tggtagccca tgctaaaatt atgcacttcc
    68101 catttggcat ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc
    68161 ggttcttcgc tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc
    68221 cccatcgctt tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc
    68281 tcttcataga caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccagtggta
    68341 ttgatttgca ggaactcatc gcccgaagtg tagtaacgga aaatctggct acccccgccc
    68401 agatcgatgt ggctgacagc ggctaccgta aattcctcac ccggcagcgc aatcagcaat
    68461 tcatcttcca gcaaacgaaa cgataatgtg tcgagcgtaa agccactatt gagatgcaga
    68521 cccagcggac cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa
    68581 ccagacatat tttatcctta aaatagttac cagccttgca gtgaggctgg caaaaaatta
    68641 ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc
    68701 gccaattcca gcttccgcca gcttttcatc gagatcgcga ccgtccgcga ctttttccaa
    68761 ctgtgcggca gcatccaggc gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa
    68821 ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg gtagaggtcg ttacagcctg
    68881 ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc
    68941 gatacgctgc gctgtcgctg taactgcttt ttccacgcca tcacgagaga cttccagatt
    69001 cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac
    69061 ttcgttaatc aacgacggat taacgttctt gcttaacgct tccagcgcac gcgcttccag
    69121 actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca cccgtgccag
    69181 cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg
    69241 ttccagcatg cgcacgccct gggtttcttc aatggactct tccgcctggg agataaacga
    69301 tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg
    69361 tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc
    69421 aagcaggata tcttcaagag aagatttaag cgataacgca ccgaaaacaa tgtaatactc
    69481 ttcctgttgc acgctggaga tccctaccga tgacaacggc atcatcttct gatttcttaa
    69541 taagaaggta ttaaattcat ctggattgct gatactactt accggacaaa taaaggtttc
    69601 gatgatcatt tggcgggaag taaaaagaat attgatttgc aaatcgccat aatcattcag
    69661 tttaataatt aatgcacttt cgttatttgt tacatcaata ttttgttcag gtacagtttg
    69721 cagcgccgtc gccagcgcca acggattcca tgtcatatat tattccatat agattaagtt
    69781 taaatattaa taaaatgaat atttgcagta cgtaattatc ttaccagcta tagacaaaaa
    69841 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
    69901 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
    69961 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
    70021 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
    70081 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
    70141 cgccagatta gtcacccgaa tcagcggaac gctttctgcc gcaccacagg ccactttttt
    70201 cgccgtggcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacaccagc
    70261 ggcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
    70321 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
    70381 ccgtcctggc ttcacacgag cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
    70441 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
    70501 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
    70561 ggcgcgctcc agcagggcct gcactgcatg gatgccgtaa atcatttcgc tcattaatgt
    70621 actcgttgtt tacgttttgc cgggagcgcc acgcttgccc ggcctacgga taattcaccc
    70681 tcttctgttt gaagagggta atgatcactc tgccactttt ttcttcgccg cacgcttcgc
    70741 tttggtcgct gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc
    70801 tttcttcgct gctttcggct tcggtttttt ctcgccacgg aaggcgctgt ctggctcaaa
    70861 gttgaccttt ttgccgacct gacgccgctt gccgcctttt ttgcctgcat cgcctttttt
    70921 cgctttctcg cgcgccgttt taccgacgtt gcgcggtgcg cgttcgctgg agatcaggct
    70981 aaagtcgatt ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc
    71041 caggcgataa gtctgaccgc tggattcacc catcaggcgt tgccctacct ggtcaaagcg
    71101 atagtagtca ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag
    71161 acggacgaag aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac
    71221 ctggtcgagc atgaagtcac acttcagcca gtcagacaca tcgcgcgttg cttcatcggc
    71281 acgacgttcc gccatcgaac agtgctgacc cagttgcagc atctcttcca tcgaataatg
    71341 gtagccgccg gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatattt
    71401 aatggcgcgg tgcaacgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata
    71461 ggactgcaat gccagaccga agtgaccacg gttttccgga tcgtaaatcg cctgtttcat
    71521 cgagcgcaaa agcatggttt gcagcatttc tgcatcagga cgatcggcaa ccgattccag
    71581 taactctgca tagtcgcgcg gttccggctt gttaccgccc ggcaattcca gccccagctc
    71641 cgccagcact gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa
    71701 cagtgccggt tctttggctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat
    71761 gcactcttca attaacttgt gggcgtcgtt acgctgagtc tgttcgatac gttcaatacg
    71821 gcgttcaacg ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc
    71881 ttcacgggct ttatccagca ctttatagag gttatgtaac tcttcgagat gcttaaccag
    71941 cggcgcatac tgctcgcgca gatcctgatc gccttgcagt atatgccaga ctttggtgta
    72001 ggtcagacgc gcgtgagaac tcatcaccgc ttcgtagaat ttgtagccag tcaggcggcc
    72061 tttcgacgaa accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga
    72121 gcacaggccg ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac
    72181 cgacgtacca cggttacgcg cttctctgtc cagcggtgtt ggcggacgga catagtaact
    72241 gacgtcggca attgcgaccc acaaacgcca gccaccgccg cgttttttct cacagtaaac
    72301 tgcatcgtca aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcag
    72361 atcgacacgg cccgcttttg cttcttccgg cacttcttct ttcagcccgg caacctgttg
    72421 ctcaacagcc tgcggccaga tgtacggaat ttcatgggta cgcagagcaa tatcaaccgc
    72481 catgccggtg cccatattat cgcccagcac ttcgacgatt ttacccaccg ctttggtacg
    72541 gcgagtcggg cgttgagtca gttcaaccac caccacaaag cccatccgcg cgcccatgat
    72601 ctgatcgggc gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc
    72661 gacgcccgct tcggtgaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg
    72721 gacaatacgc gcttcacgac gacctttacg gtcagcgccc agcggctgag ccagcacctg
    72781 atcgccatga atgcaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc
    72841 ttcaactcgc agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc
    72901 gaggcgttcc ggcagcgcat agcactgacg gcgagtgaag accagttgac catcgcgctc
    72961 catcgcgcgc aggcgtcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc
    73021 taccgccagc tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa
    73081 ttcccgacta gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg
    73141 tgacatctcg gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat
    73201 aatttataaa gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc
    73261 gtcagaaaac tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg
    73321 tggcaaaact cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca
    73381 ccaatacgta tcgcactcgc cggtttaccc aagcgaatgc cgccattttt tccacgaaca
    73441 gcagtcacgt agccggcacg actaagttga ttgattattt tgaccatatg attacgggag
    73501 acgccgtaga cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc
    73561 atgtagatca gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca
    73621 aaagggaaat cggggaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg
    73681 ctaatttatt tcagttcggg gaaggaaaaa aacgaggatt taacactgtg tcggatagcg
    73741 taatggctta accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc
    73801 catgcgtacc agaattacgc gtcgaacggg tcgcgcagaa tcatggtttc agtacgatcc
    73861 ggaccggtag agatgatatc gatcggcaca ccggtcagct cttcaatgcg cttgatgtag
    73921 ttcagtgccg cctgcggcag gccactacga tctttcacgc cgaaggtgga ttcagaccag
    73981 cccggcatgg tttcgtaaat cggctctaga cctttccagt cgtcagctgc cagcggagtg
    74041 gtagtcactt cacgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg
    74101 ccatccagaa cgtccagttt agtcaggcag aagccagaca gggagttcaa ctgtaccgca
    74161 cgacgaacgg caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg
    74221 aattcgttac cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac
    74281 ggacctgccc ccacacgagt ggagtaagct ttgaggatac ccagaacgta atcaacataa
    74341 cgcgggccca ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg
    74401 tacggatatg taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg
    74461 aaatcgccac gctgacgcgc ctggtcgagc aggtcagaaa cgtcaacaac catagaagtc
    74521 aggatgtcgg caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct
    74581 ttgtagtagt taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg
    74641 aaggtttctt tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca
    74701 taagcaggcc cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca
    74761 cgcgcgttat ccagcgcaac gtgataatca aggatcagcg gacatgcttc ggacagcagc
    74821 agacgctcac gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgca
    74881 gccggagaca gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct
    74941 gatggaataa gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg
    75001 tgaccgccct ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta
    75061 cctttacctt cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa
    75121 aatcaccgtt tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt
    75181 gcactcaaaa atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat
    75241 gtagtagacc acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc
    75301 gggcaaattg gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc
    75361 taaaccttcc agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta
    75421 tccttataga aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt
    75481 accgcagtcg ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc
    75541 gctgtccggg ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg
    75601 caggctacgg atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt
    75661 ggctgcttct gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag
    75721 cgttctggtc acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg
    75781 tgaacggtga cgacgtgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc
    75841 agacacttcg gtcggcaggt tgatctgctt aattcgcaca tcgacaactt caatacccag
    75901 cgccgccata ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg
    75961 ctctgccgct tcggcaatgg cgttatctgc tgcaggggta gtaacttcat cttctgtacc
    76021 cgcagaaccg gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt
    76081 gacgatatct ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg
    76141 tttcaacagc acttccgctt gcgaaatgtc gccaccaccc gttgccagat agtaacggct
    76201 gaaatcgctg atgcgccatt tgatgtaaga gtcgacgatc aggtctttct tctctttggt
    76261 cacaaagcgg tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt
    76321 ttcaatgaac ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc
    76381 gtcacgcagt accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa
    76441 gacagacatg taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat
    76501 cgttattccc cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata
    76561 atatcgccct gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct
    76621 ggcggcagac gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttacca
    76681 cctttcagca tctggtctaa tggcagaacc atcaggttgc cacctttatc gttaaccagc
    76741 actttgcggg tgttgcccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta
    76801 atttccggcg cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga
    76861 gcttccagga tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga
    76921 ccgttcgcac gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt
    76981 tcgttttcac gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga
    77041 gcagcctgga agttgacgtc cagcagcgtg atacccatgt catacggacg aatcgtctct
    77101 tccagttcgc gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc
    77161 atggtgtatt taccgataac cccgcgtagg gcgctgtcgg tagcctgacg caggctgtca
    77221 tccgggctgg tcacgctata cagatatttt tccggatcgg tgacgcggta ctgcacgttc
    77281 atctcaacgc gcactacgtt ctcgtccgac gtcagcatca caccagaagc tgccagttca
    77341 cgcacggctt ccacgttcac cggtttgact tcgtcaataa acgtcggttt ccagttcaga
    77401 cccggctcaa ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct
    77461 ttaatggtat agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta
    77521 acgacgcgac cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg
    77581 gtgcctttac cgccacccag accaccgagc tttttgctca gtttgcggaa gatatcatct
    77641 aaatcaggtg gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca
    77701 ggtttgctgc ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc
    77761 atgtttgtgc tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata
    77821 tgactccacg cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg
    77881 tttacagagg cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc
    77941 ctcctccatc cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgccc
    78001 ttcctgcggc ggcagacgca atgtatgctg cgccacctcg ccggaaagac gctccgtcaa
    78061 agcctgaaaa agctgtggta tccccgctcc ggtctgcgca gaaagccaga cacggatcgg
    78121 tttgttctct tcatctctgt caatacgcgg ttcgaaatcg tccagcatat cgatcttgtt
    78181 catcaccagc agggttggga tctcgtgagc atcaatctct tcaagaaccg tattcaccgc
    78241 ttcgatgttt tcttgtacac gcacatccgc cgcgtcaatg acgtgcagca gcaatgtggc
    78301 ttgccgcgtc tcttgtaacg tggctttaaa tgccgccacc agatcgtgcg gcaggtggcg
    78361 aataaaccct acggtatctg ccaacacggt ttcaccgaca tccgcaacgt caatacgccg
    78421 caacgtcggg tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat
    78481 gcgattgaaa agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac
    78541 atcggctttg atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttccaggcg
    78601 cgactgtatc tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc
    78661 acccggacca cgcaaaccga tcccgccttt ctgtctttca aggtgggtcc agccacgcac
    78721 caggcgcgta gccagatggc gcaactgcgc cagctcaacc tgcaacttac cctcatgggt
    78781 acgcgcacgt tgggcgaaaa tatctaaaat aaggccggtg cggtcgatga cacgacactc
    78841 gcacaaacgc tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac
    78901 cgaagcaccc gtcgctttta cggcttccgc aatttcaact gctttacctt cacctacaaa
    78961 atactttggg tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc
    79021 ggaagagacc agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata
    79081 gatgtgtacc agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct
    79141 ctaaatagat cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa acagcccgaa
    79201 accttattcg gtttcttcgc tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg
    79261 atgatagtta ctgctggtac cgccaccggc gttgttactg tgatgagaaa ccgggcgaga
    79321 cgggacaaca gtagaaatcg cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag
    79381 gatcacgaac tgatcaaaag actcgatttg tccttgcagc ttaataccat tcaccaaata
    79441 aatagaaact ggaacacgtt cccgacgcag tgcgttcagg aacggatctt gtaaagattg
    79501 ccccttagcc attctctctt ttccttatat gcttatttgt actttgaacc tttcgattct
    79561 gaaaaaattg cgcacgatac gtctcaattg tacacattca gcctgcgata gcaccaacaa
    79621 cctgtaatac ttcgtcacgc gcctgttctg gtttttcact gtcaagccag tgaacccctt
    79681 cccaaccacg cagccaggtt atctgccgct tcgccaactg tctcgtggcg caaacacctc
    79741 gataaaccat ttcatcgtat gagatttcgc cttcaaggta agaccacatc tggcgataac
    79801 ccacgcaacg aatggaaggc aaatccgtat gcaaatctcc tcgggcaaaa agcgcccgga
    79861 cttctgcttc aaaacctgaa gccaacatct gatgaaaacg ctgctcaatt cgttgatgga
    79921 gcagttcacg gctcgccggg gcgatggcga actgatgcac ctgatacggt agagcgtctc
    79981 ctgacgtttg cgtcagttcc gttaaagttt tacccgaaat gaaaaaaact tccagtgccc
    80041 gggaaagcct ttgtggatca tttggatgaa tccttgctgc cgcaaccgga tctacctcct
    80101 gaagttgacg atgcaatgac tcccaacctt gctctgccgc ctgttgctca attctggccc
    80161 gtacttccgg gtccgccgac ggtagcggcg acaacccttc cagcaatgcc ttgaaataca
    80221 acatcgtacc gcccactaat agtgggatcc gccccgccgc ggtgatatcg gccatttccg
    80281 ccagcgcatc gcggcgaaaa tcagcagccg agtaagcctg cgacggatcg cgaatattca
    80341 gcaatcggtg cggcgcggcg agtaactctt cagcgttcgg cttcgccgtc ccgatatcca
    80401 tccctttgta aataagggca gaatcaacgc ttatcaactc tactggtaaa attttacgca
    80461 gctcaatggc taacgctgtt ttaccggagg ccgtcggccc catcaaaaaa atcgccttag
    80521 gcaggctcgc cttactgata tcactcatct ttcagggctt ttatcgccgg atgtaaatca
    80581 acagattgta acagaccacc cggcggcgtt ttcacaagtt gcggacataa ccgttccacg
    80641 tccgccagca gggttatggc ctgtgccatt gaccactgcg catgttcgct catcagattt
    80701 cgtgcaatcc actgcgcaat attgccaggt tcgaatacgg actgcttcgc caggtagcct
    80761 atcagttcag gaatcaagat ttgtaaattt tgttggcgta agggtaaagg cacggccctg
    80821 atggtcacat gctgcgcatc ggactggaaa tcaataccca attccgccag ggcagactgc
    80881 gctttttcta atgccgattt ttcttcgcca gaaactttta gccgcaacgg aataagcagc
    80941 ggttgggcgc aaactggcac ttcacccggc gtcaattgtg cctgacgcag ccaacgttct
    81001 gccactggca aggataaaag tgaaatgttg ccatcgcgct caagcaacgc acagtcggaa
    81061 tggacgatag tcagtacccg accaaaactc tgactgttcg ccgcaagtgc aggttcctgc
    81121 ggttctggcg cttttggttt ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact
    81181 tcaccttgct gtttctggta acctggctgc gcattcggcc agggggcagc cggacgactg
    81241 cctgaggagg gcgcaggagt gtaacgcgga gctactggct cacgaactgc cggttcagca
    81301 aaatgattgc gccccgccgc cacgcgattt tccggaatgg cacgcggtgc aggttgtggt
    81361 tcatcgtcca gcggtagcgg cgtttccagt tgctgttgta gcacgctcag cacgccctga
    81421 tagataaagt catgcaccag acgcgactga tggaaacgca cttcgtgttt ggcagggtgc
    81481 acgttgacgt ccacctgatg cgggtcgatc tccagataca acacaaatgc cggttgctga
    81541 tcggccccca gtttgtcttc gcaggcctgg cggatcgcgt gattgatcag gcgatcgcgc
    81601 atcatgcgac cgttcacgta gcaatactga atttctgcca gtgcgggcgt ggtgtgattt
    81661 ggatcggcca cccagccgcg cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct
    81721 tgttcgagaa aagcggtgcc gcaaatcgcg cctaagcgcc gttctttttg cccgccttcc
    81781 ggcactgcgc ggtactggcg tacaattttg ccgttatgcg acaggttgat agtgacgtcg
    81841 aaacgcgcca gcgcaatgcg gcggatgatt tcatcgatat ggttaaattc ggttttctcg
    81901 gtgcgcagga atttgcgccg cgccggggtg ttgtagaaca ggtccagcac ctccagcgtc
    81961 gttcccaccg gatgcgccgc cggtttaacc gtcacgtcca tattgcgccc ttcggcataa
    82021 gcctgccagg cttcctgctg ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa
    82081 ctgatactcg ccagtgcctc accgcgaaag cccaggctga taatggcttc gagatcgtcc
    82141 agagaggcga ttttactggt ggcatgacgc gccagcgcca gtgccagctc atcttttttg
    82201 ataccgcagc cgttatcacg aatgcggata agtttcgccc caccgcgttc gatatcaata
    82261 tcgatacgcg tcgcacctgc atcgaggctg ttttccacca gttctttgac taccgacgca
    82321 ggtcgctcga ccacctcacc tgcggcaatc tggttcgcca gttgtggcgg taagacctga
    82381 attggcatca atcgtcctta gtttggcagc gtgcgatctg gcgtcgtcac cgtactggcc
    82441 gtttgtgccg ttgcaccctg cggcgcagat tgcatcggat gcgcaaggaa gtaattgcgc
    82501 aggcctttgt aaatggcttc tgccagctgt tgttggtaat cgtcgctcgc cagcaaacgt
    82561 tcttcgctgt tgttactgat aaaaccggtt tcgaccagta ctgacggaat atccggcgaa
    82621 cgcagaacgc cgagactcgc gtgttctggt cggcgtttat gtatttcgcc aatgcgttgc
    82681 aactgactga tcatactggt cgctacatca taccctaccc gctgggaatg accgaactgt
    82741 aaatccagca ccgcctggct taaatagggg tcggcctgac tgttcgccag cacatcaccc
    82801 gccccgccca gcagctccga ctgcttctca tgctgttcca gccagctggc catttcgctg
    82861 ttggcgcgac ggtttgagag cacccataca gaggcaccgg tggcactacg gtttggtgcg
    82921 gcatcggcat gaatcgacac aaggaaattg gcgttttgct tacgtgccac atcactgcgc
    82981 cccatcaccg agataaagta atccccgtca cgggttaaaa cgcccttaaa catcggatcg
    83041 ttattgagca aggtacgtaa tttacgcgca atggcgatgg tgacattttt ctcccgtgta
    83101 ccaccagggc cgatagcgcc agggtcctga ccgccgtgtc cggcatcaat agcgatgata
    83161 attttatcgc ctgtgttagc cgtcgcgcgc gctgccggac gcgttaccgt attactgctg
    83221 ataacacccg tagtgcggtt actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg
    83281 cgcggtgcga caaccgcagg cgtttcaacg cgtttcgcaa ccattggcgg aggcgatggc
    83341 ggcgcatcgg cgttaatcgt aaagacaaca gtgtaattgc tgccattctg ccgcttcacc
    83401 gcttcggttt taccgttttc ggtgagatcg accaccagcc gtagcgtttg tgcatcttta
    83461 ggcgttccgg agcgaatcgc tttcaccagg ttattaccgc taaacaacaa cggcagcccc
    83521 tgaattaccc ccgtttgttt gatatcgagc gccacggtgc gtttgctttg atggctaaac
    83581 gcataatcag gatcgccaat aaaactcaac gttatccgcg cctgttgatt accgttagaa
    83641 acctgaatat cagagagcgt cgcggccccc gcctgcgcgc acagcagcag caacgtcgct
    83701 actaaccaat ttctgatgcg atacatcatc ccgccacctt tcaaaggtta accggctaaa
    83761 cgcgccagca acaattcacc cgcagaggaa accgcactca cgcgcgcctc acggccttgt
    83821 gcctgataat caatgtgtat ttcgacatcc gggtcaggaa gaacacctgt accttgttgt
    83881 ggccactcca ccaggcagat ggcatcgttg gcaaaataat cgcggatccc cataaactcc
    83941 agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc
    84001 gtatagggtt cgaccagcgt ataagtgggg cttttgacat taccctgatg acccagagcc
    84061 tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta aatcgccata cagatagatt
    84121 acggttgcgc catcgcaggc tttcgctacc cgctcgccca ggtctaatgt tgcctgctca
    84181 tcagggagcg gaattactcg attcatcatg gtttttatca gtcacttccg ggttaacaat
    84241 acgctgtagc gtggaaaaga gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc
    84301 cagtacgtca gctgccgcac cgtgcgcgac acagcccaca caggctgcat cataagggct
    84361 cattttttgc ccaagcaatg cgccaataat accagagagc acatcgccca tgccgccgct
    84421 cgccatgcct gcatttccgg catcaataat gcctaaagcg tcaggatggg cggcgaccac
    84481 ggttccggca cctttcagca ccgctacgcc gccataacgt tgtaccagac gttgggcgca
    84541 atgtaagcgg tcactttcaa tttcagcgac ggaacatcct aacaaccgtg cggcctcacc
    84601 aggatgcggc gtgatcacgc gattgtgacg cttatcggga ttgattgcca gcaggttcaa
    84661 tgcatcggca tcccacaaca tcggtttgcg aaaattctca actttttgca gggctttttt
    84721 cccccactct tgctggccca gaccgggacc aatcaccacc acatcggccc attccaggct
    84781 ttcggtaaga gagcccatag tcagttcatg caccatcaat tccggtcgtg cagtcagcag
    84841 cggcgcaatg ttctcactgc gggtcagtac tcggactaaa ccagcaccag cacgcagcgc
    84901 cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga tcgccaccaa taatcaccag
    84961 ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt atcagccact gagaaagttg
    85021 ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc agccatctat ccagccgcag
    85081 tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt ccagtgagca aacctggttt
    85141 cagcgcaata aaagtgatgg tgtgatcggc gttgattacc gcgcctggcg tagcgccggt
    85201 ttcagccaga aggccggaag ggatatcaac cgccacaatc ggcgcaggat gggaattagc
    85261 gtggtcgatt aactggctaa tggattcgcg gggcgcttgc tgcaaaccgg tgccgagcag
    85321 cgcatcgaca atcagatcta ccgattcggg ccagacaata ttcgaagcat ggatctcgcc
    85381 tcccgcgttt aaccatgctt cgcgtgccag cgcggcctct tccggcaacg gtttgtcact
    85441 ttcctgggcc agcagcgtga catcaatacc gatcgctttg gccagccgcg cgaccacgta
    85501 gccatcgccg ccgttattac catgaccgca cagcaccagc cagtggcggg cgtcaggata
    85561 agccgaacga cacacctgga atgcggcttc gccagcgcga agcatcagct catagagtgt
    85621 gagccccagc gcatctgccg cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac
    85681 ggtgtgtggt atacttacgg ggtttttctt cattgtatgg tccgtcatgt cagagcccct
    85741 cgatctcaat cagttagcgc aaaaaattaa acagtggggg ctggaactgg gctttcagca
    85801 ggtaggtatt accgataccg atctcagcga gtccgagccc aaactgcaag catggctgga
    85861 caaacaatac cacggcgaaa tggactggat ggcgcgtcac ggtatgctgc gtgctcgtcc
    85921 tcacgaatta ttgcccggta cgctgcgcgt gatcagcgtg cggatgaatt accttcctgc
    85981 taacgccgca tttgccagca cgctgaaaaa tcccaaactc ggctatgtta gccgttatgc
    86041 gctgggccgt gactatcaca agcttctgcg caaccgactc aaaaagctgg gcgagatgat
    86101 tcagcaacat tgtgtttcgc tgaattttag accgtttgtc gattctgcgc ctattctcga
    86161 gcgcccgtta gctgaaaaag caggtcttgg ctggacaggt aagcactcac ttatcctcaa
    86221 tcgcgaggcc ggttcgttct tctttttagg cgaattgctg gtcgatattc cgctgcccgt
    86281 ggatcaacca gtcgaggaag gatgcggtaa atgcgtggcc tgtatgacga tttgcccgac
    86341 cggtgccatc gtcgagccat ataccgtcga tgctcgccgc tgtatctctt atctcaccat
    86401 cgaactggaa ggggagatcc cggaagagtt gcgaccgtta atgggaaacc gtatttacgg
    86461 ttgcgatgac tgccagctta tctgcccgtg gaatcgctat tcgcaactca ctacagaaga
    86521 cgatttcagc ccgcgtaagc cgctacacgc accggaactc attgagttat tcgcctggag
    86581 tgaagagaag tttttaaaag tcacggaagg ttcggcgatt cgccgtatag gtcacctgcg
    86641 ttggctgcgt aatatcgccg tagcattagg caatgccccc tgggatgaaa cgattttgac
    86701 agcgctggaa agtcgtaaag gtgagcaccc acttcttgat gagcacatag cgtgggcgat
    86761 tgcgcagcaa atcgagagac gaaatgcgtg cgtggtcgaa gtgcaattac cgaaaaaaca
    86821 gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac gcctgattca tccacagctt
    86881 gtgaataaaa ataaaaacgc attgcaattc aagccggaaa aaatcgtcaa gtgatcgata
    86941 cgacaaaacg aaatcttaat ttt
//
LOCUS       DAJOTB010000004        85606 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJOTB010000004 DAJOTB010000000
VERSION     DAJOTB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.004"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            34..510
                     /gene="agaB"
                     /locus_tag="OHN28_000246"
     CDS             34..510
                     /gene="agaB"
                     /locus_tag="OHN28_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2295696.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            549..1352
                     /gene="agaC"
                     /locus_tag="OHN28_000247"
     CDS             549..1352
                     /gene="agaC"
                     /locus_tag="OHN28_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCQ2295697.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1342..2133
                     /gene="agaD"
                     /locus_tag="OHN28_000248"
     CDS             1342..2133
                     /gene="agaD"
                     /locus_tag="OHN28_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCQ2295698.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2134..2901
                     /locus_tag="OHN28_000249"
     CDS             2134..2901
                     /locus_tag="OHN28_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCQ2295699.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(2951..3814)
                     /gene="rsmI"
                     /locus_tag="OHN28_000250"
     CDS             complement(2951..3814)
                     /gene="rsmI"
                     /locus_tag="OHN28_000250"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCQ2295700.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3879..5915
                     /gene="lpoA"
                     /locus_tag="OHN28_000251"
     CDS             3879..5915
                     /gene="lpoA"
                     /locus_tag="OHN28_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCQ2295701.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YAGSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            5873..6268
                     /locus_tag="OHN28_000252"
     CDS             5873..6268
                     /locus_tag="OHN28_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCQ2295702.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6288..6878
                     /gene="diaA"
                     /locus_tag="OHN28_000253"
     CDS             6288..6878
                     /gene="diaA"
                     /locus_tag="OHN28_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCQ2295703.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6888..7463
                     /gene="yraP"
                     /locus_tag="OHN28_000254"
     CDS             6888..7463
                     /gene="yraP"
                     /locus_tag="OHN28_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCQ2295704.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7576..8616)
                     /locus_tag="OHN28_000255"
     CDS             complement(7576..8616)
                     /locus_tag="OHN28_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2295705.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8689..9324)
                     /locus_tag="OHN28_000256"
     CDS             complement(8689..9324)
                     /locus_tag="OHN28_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCQ2295706.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9452..9970
                     /gene="yhbO"
                     /locus_tag="OHN28_000257"
     CDS             9452..9970
                     /gene="yhbO"
                     /locus_tag="OHN28_000257"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCQ2295707.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9950..10393)
                     /locus_tag="OHN28_000258"
     CDS             complement(9950..10393)
                     /locus_tag="OHN28_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCQ2295708.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10444..10746
                     /gene="yhbQ"
                     /locus_tag="OHN28_000259"
     CDS             10444..10746
                     /gene="yhbQ"
                     /locus_tag="OHN28_000259"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCQ2295709.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQTPEIKSD"
     gene            complement(10733..11236)
                     /locus_tag="OHN28_000260"
     CDS             complement(10733..11236)
                     /locus_tag="OHN28_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2295710.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11230..11754)
                     /locus_tag="OHN28_000261"
     CDS             complement(11230..11754)
                     /locus_tag="OHN28_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCQ2295711.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11963..12958
                     /locus_tag="OHN28_000262"
     CDS             11963..12958
                     /locus_tag="OHN28_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2295712.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12967..13845
                     /locus_tag="OHN28_000263"
     CDS             12967..13845
                     /locus_tag="OHN28_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2295713.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13926..14933
                     /locus_tag="OHN28_000264"
     CDS             13926..14933
                     /locus_tag="OHN28_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCQ2295714.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15051..16295)
                     /gene="mtr"
                     /locus_tag="OHN28_000265"
     CDS             complement(15051..16295)
                     /gene="mtr"
                     /locus_tag="OHN28_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCQ2295715.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16449..18338)
                     /gene="deaD"
                     /locus_tag="OHN28_000266"
     CDS             complement(16449..18338)
                     /gene="deaD"
                     /locus_tag="OHN28_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCQ2295716.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18331..18411)
                     /gene="yrbN"
                     /locus_tag="OHN28_000267"
     CDS             complement(18331..18411)
                     /gene="yrbN"
                     /locus_tag="OHN28_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCQ2295717.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18518..19402)
                     /gene="nlpI"
                     /locus_tag="OHN28_000268"
     CDS             complement(18518..19402)
                     /gene="nlpI"
                     /locus_tag="OHN28_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCQ2295718.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19511..21646)
                     /gene="pnp"
                     /locus_tag="OHN28_000269"
     CDS             complement(19511..21646)
                     /gene="pnp"
                     /locus_tag="OHN28_000269"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCQ2295719.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21893..22162)
                     /gene="rpsO"
                     /locus_tag="OHN28_000270"
     CDS             complement(21893..22162)
                     /gene="rpsO"
                     /locus_tag="OHN28_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCQ2295720.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22311..23255)
                     /gene="truB"
                     /locus_tag="OHN28_000271"
     CDS             complement(22311..23255)
                     /gene="truB"
                     /locus_tag="OHN28_000271"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCQ2295721.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23255..23656)
                     /gene="rbfA"
                     /locus_tag="OHN28_000272"
     CDS             complement(23255..23656)
                     /gene="rbfA"
                     /locus_tag="OHN28_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCQ2295722.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23820..26492)
                     /gene="infB"
                     /locus_tag="OHN28_000273"
     CDS             complement(23820..26492)
                     /gene="infB"
                     /locus_tag="OHN28_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCQ2295723.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26517..28004)
                     /gene="nusA"
                     /locus_tag="OHN28_000274"
     CDS             complement(26517..28004)
                     /gene="nusA"
                     /locus_tag="OHN28_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCQ2295724.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28032..28484)
                     /gene="rimP"
                     /locus_tag="OHN28_000275"
     CDS             complement(28032..28484)
                     /gene="rimP"
                     /locus_tag="OHN28_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCQ2295725.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28691..28767)
                     /locus_tag="OHN28_000276"
     tRNA            complement(28691..28767)
                     /locus_tag="OHN28_000276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28731..28733),aa:Met,seq:cat)
     gene            29115..30458
                     /gene="argG"
                     /locus_tag="OHN28_000277"
     CDS             29115..30458
                     /gene="argG"
                     /locus_tag="OHN28_000277"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCQ2295726.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30466..32091)
                     /locus_tag="OHN28_000278"
     CDS             complement(30466..32091)
                     /locus_tag="OHN28_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2295727.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIIFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(32548..32634)
                     /locus_tag="OHN28_000279"
     tRNA            complement(32548..32634)
                     /locus_tag="OHN28_000279"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32598..32600),aa:Leu,seq:gag)
     gene            complement(32649..32981)
                     /gene="secG"
                     /locus_tag="OHN28_000280"
     CDS             complement(32649..32981)
                     /gene="secG"
                     /locus_tag="OHN28_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCQ2295728.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33209..34546)
                     /gene="glmM"
                     /locus_tag="OHN28_000281"
     CDS             complement(33209..34546)
                     /gene="glmM"
                     /locus_tag="OHN28_000281"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCQ2295729.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34539..35387)
                     /gene="folP"
                     /locus_tag="OHN28_000282"
     CDS             complement(34539..35387)
                     /gene="folP"
                     /locus_tag="OHN28_000282"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCQ2295730.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35477..37411)
                     /gene="ftsH"
                     /locus_tag="OHN28_000283"
     CDS             complement(35477..37411)
                     /gene="ftsH"
                     /locus_tag="OHN28_000283"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCQ2295731.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37511..38140)
                     /gene="rlmE"
                     /locus_tag="OHN28_000284"
     CDS             complement(37511..38140)
                     /gene="rlmE"
                     /locus_tag="OHN28_000284"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCQ2295732.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38266..38559
                     /gene="yhbY"
                     /locus_tag="OHN28_000285"
     CDS             38266..38559
                     /gene="yhbY"
                     /locus_tag="OHN28_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCQ2295733.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38715..39191)
                     /gene="greA"
                     /locus_tag="OHN28_000286"
     CDS             complement(38715..39191)
                     /gene="greA"
                     /locus_tag="OHN28_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCQ2295734.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39439..40872
                     /gene="dacB"
                     /locus_tag="OHN28_000287"
     CDS             39439..40872
                     /gene="dacB"
                     /locus_tag="OHN28_000287"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2295735.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40913..42085)
                     /gene="cgtA"
                     /locus_tag="OHN28_000288"
     CDS             complement(40913..42085)
                     /gene="cgtA"
                     /locus_tag="OHN28_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCQ2295736.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42101..43066)
                     /locus_tag="OHN28_000289"
     CDS             complement(42101..43066)
                     /locus_tag="OHN28_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2295737.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43193..43450)
                     /gene="rpmA"
                     /locus_tag="OHN28_000290"
     CDS             complement(43193..43450)
                     /gene="rpmA"
                     /locus_tag="OHN28_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCQ2295738.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43471..43782)
                     /gene="rplU"
                     /locus_tag="OHN28_000291"
     CDS             complement(43471..43782)
                     /gene="rplU"
                     /locus_tag="OHN28_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCQ2295739.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44041..45012
                     /gene="ispB"
                     /locus_tag="OHN28_000292"
     CDS             44041..45012
                     /gene="ispB"
                     /locus_tag="OHN28_000292"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCQ2295740.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45245..45523
                     /gene="sfsB"
                     /locus_tag="OHN28_000293"
     CDS             45245..45523
                     /gene="sfsB"
                     /locus_tag="OHN28_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCQ2295741.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45571..46830)
                     /gene="murA"
                     /locus_tag="OHN28_000294"
     CDS             complement(45571..46830)
                     /gene="murA"
                     /locus_tag="OHN28_000294"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCQ2295742.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46885..47139)
                     /gene="ibaG"
                     /locus_tag="OHN28_000295"
     CDS             complement(46885..47139)
                     /gene="ibaG"
                     /locus_tag="OHN28_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCQ2295743.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47299..47592)
                     /gene="mlaB"
                     /locus_tag="OHN28_000296"
     CDS             complement(47299..47592)
                     /gene="mlaB"
                     /locus_tag="OHN28_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCQ2295744.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47592..48227)
                     /gene="mlaC"
                     /locus_tag="OHN28_000297"
     CDS             complement(47592..48227)
                     /gene="mlaC"
                     /locus_tag="OHN28_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCQ2295745.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48246..48797)
                     /gene="mlaD"
                     /locus_tag="OHN28_000298"
     CDS             complement(48246..48797)
                     /gene="mlaD"
                     /locus_tag="OHN28_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCQ2295746.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48802..49584)
                     /gene="mlaE"
                     /locus_tag="OHN28_000299"
     CDS             complement(48802..49584)
                     /gene="mlaE"
                     /locus_tag="OHN28_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCQ2295747.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49592..50401)
                     /gene="mlaF"
                     /locus_tag="OHN28_000300"
     CDS             complement(49592..50401)
                     /gene="mlaF"
                     /locus_tag="OHN28_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCQ2295748.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50611..51588
                     /locus_tag="OHN28_000301"
     CDS             50611..51588
                     /locus_tag="OHN28_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCQ2295749.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51602..52588
                     /gene="kdsD"
                     /locus_tag="OHN28_000302"
     CDS             51602..52588
                     /gene="kdsD"
                     /locus_tag="OHN28_000302"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCQ2295750.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52609..53175
                     /gene="kdsC"
                     /locus_tag="OHN28_000303"
     CDS             52609..53175
                     /gene="kdsC"
                     /locus_tag="OHN28_000303"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCQ2295751.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53172..53747
                     /gene="lptC"
                     /locus_tag="OHN28_000304"
     CDS             53172..53747
                     /gene="lptC"
                     /locus_tag="OHN28_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCQ2295752.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53716..54273
                     /gene="lptA"
                     /locus_tag="OHN28_000305"
     CDS             53716..54273
                     /gene="lptA"
                     /locus_tag="OHN28_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCQ2295753.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54280..55005
                     /gene="lptB"
                     /locus_tag="OHN28_000306"
     CDS             54280..55005
                     /gene="lptB"
                     /locus_tag="OHN28_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCQ2295754.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55053..56486
                     /gene="rpoN"
                     /locus_tag="OHN28_000307"
     CDS             55053..56486
                     /gene="rpoN"
                     /locus_tag="OHN28_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCQ2295755.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56509..56796
                     /gene="hpf"
                     /locus_tag="OHN28_000308"
     CDS             56509..56796
                     /gene="hpf"
                     /locus_tag="OHN28_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCQ2295756.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56914..57405
                     /gene="ptsN"
                     /locus_tag="OHN28_000309"
     CDS             56914..57405
                     /gene="ptsN"
                     /locus_tag="OHN28_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCQ2295757.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57451..58305
                     /gene="rapZ"
                     /locus_tag="OHN28_000310"
     CDS             57451..58305
                     /gene="rapZ"
                     /locus_tag="OHN28_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCQ2295758.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58302..58574
                     /gene="npr"
                     /locus_tag="OHN28_000311"
     CDS             58302..58574
                     /gene="npr"
                     /locus_tag="OHN28_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCQ2295759.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58787..59419
                     /gene="yrbL"
                     /locus_tag="OHN28_000312"
     CDS             58787..59419
                     /gene="yrbL"
                     /locus_tag="OHN28_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCQ2295760.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59416..60144)
                     /gene="mtgA"
                     /locus_tag="OHN28_000313"
     CDS             complement(59416..60144)
                     /gene="mtgA"
                     /locus_tag="OHN28_000313"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCQ2295761.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(60141..60794)
                     /gene="elbB"
                     /locus_tag="OHN28_000314"
     CDS             complement(60141..60794)
                     /gene="elbB"
                     /locus_tag="OHN28_000314"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCQ2295762.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(61024..63360)
                     /gene="arcB"
                     /locus_tag="OHN28_000315"
     CDS             complement(61024..63360)
                     /gene="arcB"
                     /locus_tag="OHN28_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCQ2295763.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63456..64385)
                     /locus_tag="OHN28_000316"
     CDS             complement(63456..64385)
                     /locus_tag="OHN28_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCQ2295764.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            65060..69520
                     /gene="gltB"
                     /locus_tag="OHN28_000317"
     CDS             65060..69520
                     /gene="gltB"
                     /locus_tag="OHN28_000317"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCQ2295765.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69533..70951
                     /gene="gltD"
                     /locus_tag="OHN28_000318"
     CDS             69533..70951
                     /gene="gltD"
                     /locus_tag="OHN28_000318"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCQ2295766.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(71019..71483)
                     /locus_tag="OHN28_000319"
     CDS             complement(71019..71483)
                     /locus_tag="OHN28_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2295767.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(71480..72355)
                     /gene="nanK"
                     /locus_tag="OHN28_000320"
     CDS             complement(71480..72355)
                     /gene="nanK"
                     /locus_tag="OHN28_000320"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCQ2295768.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(72352..73041)
                     /locus_tag="OHN28_000321"
     CDS             complement(72352..73041)
                     /locus_tag="OHN28_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCQ2295769.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(73089..74579)
                     /gene="nanT"
                     /locus_tag="OHN28_000322"
     CDS             complement(73089..74579)
                     /gene="nanT"
                     /locus_tag="OHN28_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCQ2295770.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(74688..75581)
                     /gene="nanA"
                     /locus_tag="OHN28_000323"
     CDS             complement(74688..75581)
                     /gene="nanA"
                     /locus_tag="OHN28_000323"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCQ2295771.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(75703..76494)
                     /gene="nanR"
                     /locus_tag="OHN28_000324"
     CDS             complement(75703..76494)
                     /gene="nanR"
                     /locus_tag="OHN28_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCQ2295772.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(76602..77099)
                     /gene="sspB"
                     /locus_tag="OHN28_000325"
     CDS             complement(76602..77099)
                     /gene="sspB"
                     /locus_tag="OHN28_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCQ2295773.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77105..77743)
                     /gene="sspA"
                     /locus_tag="OHN28_000326"
     CDS             complement(77105..77743)
                     /gene="sspA"
                     /locus_tag="OHN28_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCQ2295774.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78138..78530)
                     /gene="rpsI"
                     /locus_tag="OHN28_000327"
     CDS             complement(78138..78530)
                     /gene="rpsI"
                     /locus_tag="OHN28_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCQ2295775.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78546..78974)
                     /gene="rplM"
                     /locus_tag="OHN28_000328"
     CDS             complement(78546..78974)
                     /gene="rplM"
                     /locus_tag="OHN28_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCQ2295776.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79194..80321)
                     /gene="zapE"
                     /locus_tag="OHN28_000329"
     CDS             complement(79194..80321)
                     /gene="zapE"
                     /locus_tag="OHN28_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCQ2295777.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80515..80913
                     /locus_tag="OHN28_000330"
     CDS             80515..80913
                     /locus_tag="OHN28_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCQ2295778.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81067..82434
                     /gene="degQ"
                     /locus_tag="OHN28_000331"
     CDS             81067..82434
                     /gene="degQ"
                     /locus_tag="OHN28_000331"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCQ2295779.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            82524..83591
                     /gene="degS"
                     /locus_tag="OHN28_000332"
     CDS             82524..83591
                     /gene="degS"
                     /locus_tag="OHN28_000332"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCQ2295780.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83654..84592)
                     /gene="mdh"
                     /locus_tag="OHN28_000333"
     CDS             complement(83654..84592)
                     /gene="mdh"
                     /locus_tag="OHN28_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2295781.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85027..85497
                     /gene="argR"
                     /locus_tag="OHN28_000334"
     CDS             85027..85497
                     /gene="argR"
                     /locus_tag="OHN28_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCQ2295782.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        20985 a        23118 c        21312 g        20191 t
ORIGIN      
        1 tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat tctcttaacc
       61 cgtattgata accgtctggt tcacggtcag gttggcgtga cctggacatc caccatcggt
      121 gcgaatctgt tggtagtcgt ggatgatgtt gtcgctaacg atgatattca acagaaatta
      181 atgggtatta ccgcagaaac ctacggcttt ggcattcgtt tctttactat cgaaaaaacc
      241 attaacgtca tcggcaaagc tgcaccacat cagaagatct tcctgatttg ccgtacgcca
      301 caaacggtac gtaaattggt agaaggtggt attgacctga aagatgtcaa cgtcggcaat
      361 atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga tgaccaggat
      421 ctcacggact tacgttatat taaacaacgt ggcgtgaatg ttttcattca ggacgttcct
      481 ggcgatcaaa aagaacaaat ccctgactaa atctgaaatc gccttaatat tggtttgagg
      541 taataaaaat gcatgaaata accctactcc agggattatc cctggcggcg ttagttttcg
      601 ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata atcgtttgta
      661 cccttacagg tgctattctc ggtgatattc agactggctt aattaccgga ggcctgacag
      721 agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac cctattatgg
      781 cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa acggcaattg
      841 gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc tattccgctt
      901 tctcattatt tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg gcagcgtttt
      961 cccgacttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg attgctttcc
     1021 tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg cccgcctggc
     1081 tgacccacgg ctttgaagtg gctggcggta ttctgcctgc cgttggtttt ggcttgctgc
     1141 tgcgcgtgat gttcaaagcg caatatatcc cttacctgat cgccggtttc ctgtttgttt
     1201 gctacatcca ggtcagcaac ctgttgccgg ttgccgtgct gggcgcaggc tttgcggtgt
     1261 atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa
     1321 atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat cacccgtctg
     1381 ggctttcgtt cgtcactgct acaagcaagc tttaactacg aaaggatgca ggcgggcggt
     1441 tttacctggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa accgggccta
     1501 agcgcggcga tgaaagataa cctcgaattt attaataccc accctaatct ggtcggattt
     1561 ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac cattaaaggc
     1621 ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt ctggtttact
     1681 ttgttgccga ttatggcggg gatttgctca tcgtttgcca gtcaggggaa cctgttggga
     1741 ccgattttgt ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg ctggacccac
     1801 gtcggttatt cagtcggcgt gaaggctatt gataaagtgc gagagaactc gcagatgatt
     1861 gcccgttcgg caaccatcct cgggatcaca gtaatcggcg ggctgatcgc ttcgtatgtg
     1921 catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc gctgcagcag
     1981 gatttcttcg ataaagtctt cccgaatatt ttaccgatgg cctacaccct gctgatgtat
     2041 tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac gtttgttctt
     2101 tctattgttt gttctgcatt cggcattttg taaatggaac gaggcaccgc gtctggcggt
     2161 gcctctttac ttaaggaatt tcatcctgtg caaacccttc aacaagttga aaactatacg
     2221 gcgttaagtg aacgtgccag cgaatattta ttggccgtga tccgtagcaa accggatgcc
     2281 gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattattt ggtagaaaaa
     2341 atccaccagc agcaggttga tgtcagccag ctcaccttcg tgaagctcga cgaatgggtg
     2401 gatctgccat taacgatgcc tggtacctgc gaaactttcc ttcaacagca tatcgtgcag
     2461 ccgctggggc tacgtgaaga ccagctcatc agctttcact ccgaagagat aaatgagaca
     2521 gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggactt atgcgttctc
     2581 ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaagcct gcaaccggcc
     2641 tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa aaccgctgac
     2701 cgtcccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc ccgcgaagtt
     2761 ttgttactga taactggcga aggaaaacag gatgcgacag aacgttttct cacggctaaa
     2821 gtctccaccg ctatcccggc ttcattttta tggctgcata accatttcac atgcgtgatt
     2881 gatgaaatgt gtcgtcgtta actcgcctgg tacaggcgtt gcagcgctat tcctgctgcg
     2941 acgcctgtaa tcactctccc tgctgctcca gcgcatactt atacagcgca tttttcttca
     3001 cgccgtgaat ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtaaca
     3061 gcgccagcgt gcgcagggca tcagcaggca ggtcatcttc ctgcgcttta tggccttcga
     3121 caatcagcac catttcgcct ttgcgacggt tttcatcttc ttttacccac gccagcagct
     3181 cgccaacggg cgcgccgtga atggtttccc aggttttggt tagctcacgc gccagaacca
     3241 cgtaacggga ttcgcctaat accgcaacaa tatcttccag gctatctaac agacggtggg
     3301 tagattcata aaaaatcagc gtgcgcggct ccgcttcaat agcttttagc gcatcacggc
     3361 ggccttttga tttggcaggt aaaaagcctt cataacaaaa acgatcggat ggtaaacccg
     3421 cggcgcttaa cgcagtgata gcagcacacg gccctggtag cggcaccacg cgaatccccg
     3481 cttcacgaca ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat
     3541 cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt
     3601 gttgttcgtt atgatcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca
     3661 ataaaccggt gtgacgcgta tcctcggcgg caatcagatc aacggcctgt aatacctcta
     3721 atgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctgcc
     3781 cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat
     3841 attgagcatt gcgtaaaaaa aatatcactg gatacattat ggtaccctca acattttctc
     3901 gtttgaaagc cgcgcgttgt ctgcctgttg ttttggcagc cctgattttc gccggttgtg
     3961 gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag gctgattctg
     4021 ctttttacct gcaacagatg cagcaaagtt ctgatgatac caggatcaac tggcaattac
     4081 tcgccattcg tgcactggtg aaagaaggta aaaccgggca ggcggttgag ttggttaacc
     4141 aactaccgca agaactgaat gataatcagc gtcgcgagaa aacactgctg gcggcagaga
     4201 ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa atcacacctg
     4261 ccgatttaga acaaaaccag caagcccgtt actggcaggc aaaaatcgat gccagccagg
     4321 ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg ctcggtgcga
     4381 aagaaaagca gcagaatatt gacgccacat ggcaggcgct ctcctccatg actcaggagc
     4441 aggcgaatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg ctggatttgc
     4501 agcgcgtctg gtttgataac cgtaacgatc ctgacatgat gaaagctggg atcgccgact
     4561 ggcagaaacg ttatccgaac aacccgggcg cgaaaatgct gccaacgcag ttggttaacg
     4621 taaaagcgtt taaaccagca tcgaccaaca aaatcgccct gctgttgccg ctgaatggcc
     4681 aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa aatatcggca
     4741 ctcagccagt ggcggcccag gtagctgccg cacctgccgc agacgtagca gaacaacctc
     4801 agccgcaaac cgtggatggc gttgccagcc cggcacaagc ttcggttagc gatctgaccg
     4861 gtgaacagcc tgcggcccag ccggtacctg taagcgcccc ggcgacaagc accgcagcgg
     4921 taaacgcacc cgcaaatcca tccgcagagc tgaaaatcta cgatacctca tcacaaccac
     4981 ttagccagat cttaagccag gttcagcagg atggtgcgag tattgtggtc ggtccgttgc
     5041 tgaaaaataa cgttgaagag ttgctgaaga gcaacacccc gctgaacgtg ctggcactga
     5101 accagccgga gaatatcgaa aaccgcgtca atatttgtta cttcgcgctt tcgccagaag
     5161 acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc
     5221 tgatcccgcg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga
     5281 aactgggtgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg
     5341 gtgttaacgg cggttctggt attgctttaa cgggtagccc gattactccc agagcgacaa
     5401 ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacgccaacc gatgaccagt
     5461 tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg ggtgaaatcg
     5521 cttttatcaa accgatgatc gccatgcgta acggtagcca gagcggtgca acgctgtacg
     5581 ccggttcccg cagtgcgcaa gggaccgctg gcccggattt ccgcctggag atggaaggct
     5641 tgcagtacag cgaaatcccg atgctggcag gcggtaatct gccgttaatg cagcaggcgc
     5701 tcagcgcggt gaacaacgat tattcgctgg ctcgcatgta tgcgatgggt gtcgatgcct
     5761 ggtcgttggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa atcaacggta
     5821 ataccggaag cctgacggca agcccggatt gcgtgattaa caggaagtta tcatggctac
     5881 agtaccaaca aggtcaggta gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg
     5941 ggaagcacaa gcgcgtcgct ggctggaagg caaaggacta cggtttatcg ccgctaacgt
     6001 gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa ggccggacca ccgtttttat
     6061 cgaggtgcgt taccgccgct ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag
     6121 caaacaacac aaattattac agactgcccg tttgtggctc gcgcgtcata atgggagttt
     6181 tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg ttgagtggat
     6241 taaggatgcc tttaatgacc actcataatt aaggtttaag gattagcgtg caagaaagaa
     6301 ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg
     6361 atgccatctc ccgtgcagcc atgacgctgg ttcagtctct gctcaatggc aacaaaatcc
     6421 tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc agcatgatca
     6481 accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatact gataatgttg
     6541 tcttaacggc aattgccaac gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg
     6601 cgctgggtca tgcgggagat gtattgttag ccatttccac ccgtggcaac agccgcgata
     6661 ttgtaaaagc ggtagaagct gccgttacgc gtgatatgac cattgtggca ttaaccggct
     6721 atgacggcgg tgaacttgca ggtttgttag ggccacagga tgtggagatc cgcattcctt
     6781 cgcatcgtag tgctcgcatt caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc
     6841 tgatcgataa cacgcttttc cctcaccagg atgtttaagg agaatacatg aaggcattat
     6901 cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag
     6961 tgggtactgc tgctgtgggt accaaagccg caactgaccc acgcagtgtc ggcacccagg
     7021 tagacgatgg taccttggaa gtgcgcgtga acagcgcatt gtcgaaagac gaacaaatta
     7081 agaaagaagc gcgcattaat gtaacggcct atcagggcaa agtgctgctg gttgggcagt
     7141 caccaaacgc tgaactttcg gctcgcgcca aacagattgc tatgggcgta gacggtgcca
     7201 acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa gcgtctaacg
     7261 atacgtggat caccaccaaa gtacgttcgc agctgttaac cagcgacctg gtgaaatcgt
     7321 ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggctg gtgactgaac
     7381 gtgaagcgaa agcggcggca gatattgcca gccgggtaag cggcgtgaag cgggtaacta
     7441 cggcgtttac gtttattaaa tagcagccct tgtaatgcct gatgcgacgc ttgcagcgtc
     7501 ttatcaggcc tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac
     7561 atgttttccc tcaaatcaaa ccaacagcgc cagcccgccg acaatcacac cagacactgc
     7621 caccatcgcc cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag
     7681 tgacggcaaa ctcaccgccg ggagcgtcat caacaatgcc agcgccggag cagttcccat
     7741 acctgccagc atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa
     7801 gcatcctgcc accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc
     7861 atgggggaat aaccagacgc gagcggcacc caacaccagt actgcaagga tgtaaaccgg
     7921 gatcgtactc cagaaaagcg tccatagcgc cctgccccag cggctaaaaa atcctccctg
     7981 tgcttccggt atatcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt
     8041 ttgcaccagc gtcgcaatca gcaacaccat caccagcccc gccaccagac gaatcgccgc
     8101 aaaaccccag ccgaggacga accccataaa caccagcgtc gccgggttta acaacggatt
     8161 ccccatccag aatgccagcg caccgcccat cgacacctgt tggcgacgca ttcccgccgc
     8221 gaccggagcc gcacagcagg tacacatcat acccggcagc gaaaacagcg ttcccagcag
     8281 cgtgccgcga aagcgcgatt gcccaagcgt acgcaacaac cagtcacgcg ggatcagtac
     8341 ctgaatcaac gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata
     8401 gatcatcgcg taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga
     8461 tttaccgata ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt
     8521 cacataccag agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat
     8581 gggcgttgcc gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg
     8641 ataattgagc gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct
     8701 tttgcgtaat tctgaagagc ttaaaatcgt cacaccttca tgctcaggcc gcatcgcctc
     8761 tgccagcatg acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggtaacaa
     8821 acggaacagc ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cacccagtaa
     8881 catcgacggg cgagcaatgg tcaatttcgg ccagttctgg gcgattaatg cttcttccat
     8941 ctcccctttg acgcggttat agaaaaacgg cgagtgggca ttagcaccca tagcactgac
     9001 caccagcata tgctgcgcac cgagccgccg cccggttaat gcggtatcca ccaccaacgt
     9061 gtaatcggca tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca
     9121 acaaaacaca atgtcgatgg gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc
     9181 atggggatta aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cagcaatgac
     9241 gttaactttc ggttcattaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc
     9301 gcctgtaatc agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc
     9361 ccagctcatg caccaggctt aatagaccat gaggtaatta tccccgattg tggaaaattc
     9421 gcctcatcca atgcaacaac gcggaggaag catgagtaag aaaatcgccg ctttaatcac
     9481 tgatgaattt gaggattcag aattcacttc acccgcagac gagttccgta aagccggaca
     9541 cgaagtgatt accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc
     9601 cagcgtggtc atcgataaat ccatcgatga agtgacgccc gcagagttcg atgccctgct
     9661 gctaccgggc ggtcattcac cagattatct gcgtggcgac aaccgttttg tcaccttcac
     9721 tcgcgatttt gtgaacagcg gcaaacctgt tttcgccatc tgtcacggtc cccagttgct
     9781 gatcagcgcc gatgtgatcc gtggtcgtaa gctgaccgcc gttaaaccga tcgtgattga
     9841 tgttaaaaac gcgggcgcgg aattttacga tcaggaagtg gtggtcgata aagatcagct
     9901 ggttaccagc cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct
     9961 cggtgcctga gttacgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga
    10021 atttgatttc atcgagccgg atttcccata ccggggccga cagcattctg gcaaccggaa
    10081 agcgacgatt gtacgccttg cgtgcgaggt cgctttcctc gccttccagt ctgcggatct
    10141 cacctttaaa ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc
    10201 cggcaattgc cgcctgcggc ccgctcatct gcgcatgacg cgttttttct tccgtcaaaa
    10261 tgtagaaggc gactttctgc gcatcaaaaa gataaaaggc attggcgcac cacagttccc
    10321 cttcctgctg cacacaccag gtgacaacat gttgttttgc cagccaacgg ctgatggcgg
    10381 tgagtgtttc cattgctgtt ctcttttata ctgtgggcct gaccttaaca tgcgacgcag
    10441 acgatgacac cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg
    10501 atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca
    10561 ctgcgaggga aaggagaact aacgctggcg ttttccgcgc cagtgggcga ccgttcgctg
    10621 gcactacgag cggaatatcg cgttaaacaa ctgactaaac ggcaaaaaga gcgtctggta
    10681 acggaaggcg cagggtttgc agaactgttg agcagcctgc aaaccccgga gattaaaagc
    10741 gattgaaatg ctcgtgatac tcgaccagac cagttacgcc gttcagcgcg tcatccgcta
    10801 aacgatgtac ctggaaggcg ctttcagtgc ccggccagcg gcaatgcaga tcgtgatgcg
    10861 ccgccagttc aaagccgaaa cggctgtaca gcgccggatc gcccagcgtc accactgcgg
    10921 catagccgaa ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct
    10981 gtccacggta tttttcatca accgccagcg gtgccatgcc gacccattgc aggtcttcgc
    11041 cctgcacatc aaccggacta aaagccacat agccaatgac ctgaccttcg tcatctgtcg
    11101 ccaccagccc cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg
    11161 catcactttc gaatgaacga cgcagcaggg catcaatacc cggcgcatca atgggaattt
    11221 ctactcgaat tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat
    11281 tcccgcctca acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg
    11341 ctccagttca atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc
    11401 aatcaccagc cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat
    11461 cagcagatcg ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctaacgac
    11521 cagtttgcca ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact
    11581 taaccagcga ccttcaagaa actccagctc gccatcatcc agcgcctgac ggaattgcca
    11641 gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac
    11701 actcaacaga gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt
    11761 tactccctgt ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata
    11821 aatcaacaat tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc
    11881 aaaaattgtc gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgat
    11941 agcgcaacct tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct
    12001 taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgataccaa
    12061 tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt
    12121 tgtccatcaa catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg
    12181 ttacgcccgt tggcagcacg ccgtggatat ggcggcgcaa ctgggtgccg acgcgctgat
    12241 cctcgccgac ctcgccatgc tggagtacgc cgccgaacgt tatccgcata ttgagcgcca
    12301 cgtatcggtg caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt
    12361 tgacgttgcc cgcgtggtac tgccgcgcgt gttgtcgatt catcaggtga aacagctggc
    12421 acgggtcaca cctgtaccac tggaagtgtt tgctttcggc agcctgtgca ttatgtcgga
    12481 aggccgttgc tatctgtcgt cgtatctgac gggtgaatcg cccaacaccg tgggggcatg
    12541 ttctccggcc cgtttcgtgc gctggcagca aacgccgcaa gggctggaat cccgcctgaa
    12601 cgaagtgctg atcgaccgtt atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa
    12661 agggcgttat ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa
    12721 taccctggaa ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg
    12781 ccgccagcgt agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga
    12841 ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg
    12901 gtcgatgtcc gaaggcacgc aaactaccct tggcgcgtat caccgtaaat ggcagtgaga
    12961 aaagcaatga aatattcctt agggccggtg ctatggtact ggccaaaaga gacgctggaa
    13021 gacttttatc agcaggccgc cgccagcagc gcagacgtga tttatcttgg tgaagcggta
    13081 tgcagcaagc gccgggcaac caaagttggc gactggctgg agatggcaaa atcactcgcc
    13141 ggaagtggta agcagattgt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg
    13201 ggcgaactga aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc
    13261 gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc
    13321 tacaacgcgg tgacgctgaa aatattgctt aaacagggca tgatgcgctg gtgtatgccg
    13381 gtggagcttt cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttgggcatt
    13441 cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgca
    13501 cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt
    13561 aagtatccga acgggcgcaa cgtactgtcg caggaaaacc aacaagtgtt tgtactcaac
    13621 ggcattcaga ccatgagcgg ctacgtttac aacctcggta acgagctggc atccatgcag
    13681 gggctggtcg atgtggtccg cctgtcaccg cagggtactg acactttcgc aatgctcgat
    13741 gccttccgcg ctaatgaaaa tggcgctgcg ccactaccgt tgacggcaaa cagcgactgt
    13801 aacggctact ggcggcggct ggccgggctg gagctgcaag cgtaagtata tagctcactt
    13861 tgttaacaac tttaactact ctttaatgca gtattaaaga ctaatcggta acaaagtgag
    13921 ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc atccccgaag
    13981 gttcttcagc gcgggaagcg ttctcccact ctctcgacct cgcccgtctg gctgaaaagc
    14041 gcggctatca tcgctactgg ctggcagaac accacaatat gaccggcatt gccagtgctg
    14101 caacgtcggt gttaattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg
    14161 gcggcgtgat gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctta
    14221 atacgctcta tccggggcga atcgatttgg ggctgggtcg cgcgccgggt agcgaccaac
    14281 ggacaatgat ggcgctacgt cgtcatatga gcggcgatat tgataatttc cctcgcgatg
    14341 tcgcggaact ggtggactgg tttgatgccc gcgatcccaa tccgcatgtg cgccctgtac
    14401 cgggctatgg cgagaaaatc ccggtgtggt tgctaggctc cagcctttac agtgcacaac
    14461 tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgctgt
    14521 tccaggcgct gcatctttat cgcagcaact tcaaaccgtc ggcacggctg gagaaaccgt
    14581 atgcgatggt gtgcatcaat attattgccg ccgacagtaa ccgcgacgct gagtttctgt
    14641 ttacctcaat gcagcaagcc tttgtgaagc tgcgtcgcgg cgaaaccggg caactgccgc
    14701 caccgattca aaatatggat cagttctggt caccgtccga acagtatggc gtgcagcagg
    14761 cgctaagtat gtcgctggtg ggcgataaag cgaaagtgcg tcatggcttg cagtcgatcc
    14821 tgcgcgaaac cgacgccgat gagattatgg ttaacgggca gattttcgac caccaggcac
    14881 gcctgcattc gtttgagctg gcgatggatg tgaaggaaga gttgttgggg tagtgtgttt
    14941 taacgtagga agccttatcc gagatggcaa cgctgtccta cgtaggcctg ataagcgaag
    15001 cgcatcaggc attgtgtagg cagcagaaat gtcggataag gcacctctga ttactgatac
    15061 accggcagca cattaaagct cgataagata tgcaccagtg cgttgccgac gccaaacacc
    15121 agaatcagca caatcatcgg cttgccgccc catacacgga atttcgggct accaaagcgt
    15181 ttacgcgatg cgcgggccag cagcgccgga acaattgccg cccagatggt agccgctaag
    15241 ccagcataac caatggcgta caggaatccg ttaggaaaca gcaggccacc cacaaccggc
    15301 ggggcaaagg tcagcaacgc tgttttcaaa cggcccacag ccgagtcgtc gaaaccaaac
    15361 agatctgcca gatagtcaaa caggcccagc gttacgccaa ggaacgaact cgctaccgca
    15421 aagtttgaga acacaaccag cagaaggtcc aggctacggc tgttcagtac gccgcttaac
    15481 gcctgtacca gcacatcaat attaccgccc ttctcggcga tgccgataaa ctccggacgc
    15541 gggatgttac ccatcgtcgc cagcaaccag atggtataca gcgccagcgc catcagcgta
    15601 ccgtacacca ggcatttcac gatggttttc ggatctttgc cgtaatactt catcaggctc
    15661 ggtacgttac cgtgataacc aaacgatgcc agacagaacg gcagcgtcat caacagatac
    15721 ggtgcataag acgcattgct ttcggcgacg ttgaacaatg tcgcaggctg cacatgcccc
    15781 agcaggctac cgaaggtgag gaagaaggta atgactttcg cccccagcac aatcgctgtc
    15841 atgcgactga cggctttggt gctcaaccac accacaaacg ccaccaacag tgcaaaacca
    15901 aaacccgctg cccgtgccgg gacgtttagc gacatctctg cgaaggtgtg atgcagaatc
    15961 gaaccactgg cagaaatata ggcataggtc aggatgtaga gcacaaaggc aatggaaatg
    16021 ccgttgacca cgttccagcc tttgccgagc aggtctttgg tgatggtgtc aaagctcgaa
    16081 ccgattcggt aattcaggtt agcttccaga atcatcaagc cggaatgcag catacagaac
    16141 caggtgaaaa tcagcgccgc cattgaccag aagaaccacg ccccggacat gaccactggc
    16201 agagaaaaca tccctgcgcc aataatggtg ccgccgataa tcaccacgcc gccaagcagc
    16261 gacggtgacg tttgggtggt ggttagtgtt gccatgaggg cttctctcca gtgaaaaata
    16321 gtgcgactgc gttgttatgc attgcactgt accagtacat gagtacaaaa aacagaaaaa
    16381 aagccccgat gggtaaaatc ggggctgtat atattatttt acagattgtg ttcgctgttc
    16441 agcgatgatt acgcatcacc accgaaacga cgacgaccgg tagagtcatc acgacgcggt
    16501 gcgcggcctt cacgacgttc gccgctaaaa cgacgcccat caccacggcc accttcacgg
    16561 cgttcaccgc tgaagttacg accgccttca cgacgttcgc caccgaaacc acgaccaccg
    16621 ccacgacgct caccgccagt atgcggctgt gcatcgccca gtaactgcat gttcattggc
    16681 ttgttgagaa tgcgagtacg cgtaaagtgt tgcagcactt cacccggcat acctttcggc
    16741 agttcgatgg tggagtgaga agcaaacagc ttgatgttac cgatgtaacg gctgctgatg
    16801 tcgccttcgt tagcaatcgc gccaacgata tgacgaactt caacaccatc atcgcggccc
    16861 acttcaatac ggtacaactg catatcgcca acatcacgac gttcgcgacg cggacgatct
    16921 tcacggtcgc cacgcgggcc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca
    16981 cggaattcac gtttcggacg catcggcgca tctggcggaa caatcagagt acgttcaccc
    17041 tgtgccattt tcagtagtgc ggctgccaga gtttcgagat ccagctcttc accttcggca
    17101 gtcggctgaa ttttgctcag cagtgcacgg tattgatcca gatcgctgct ttccagctgc
    17161 tgctgtactt tagcggcaaa tttttccaga cgacgtttgc ccagcagttc tgcgttcggc
    17221 agttctactt ccggaatggt cagcttcatg gtgcgttcga tgttgcgcag cagacgacgc
    17281 tcacggttct caacgaacag cagtgcacga ccagcacgac ctgcacgacc ggtacgaccg
    17341 atacggtgaa cgtaagactc agaatccatc gggatatcgt agttaaccac caggctgata
    17401 cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca
    17461 tctttcaggc gttccagtgt ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg
    17521 gcgctgttgt agccgttacg ctcaagagct tctgccactt ccagagtcgc gtttttggta
    17581 cgaacgaaga taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg
    17641 tttttacgca taccccagac agtccagtag ctctggctga tgtcaggacg ggtagtcacg
    17701 ctggactgaa tgcgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc
    17761 gcttccggca tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata
    17821 atggtttcaa cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc
    17881 agaccgctca gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc
    17941 ggtgtaccga caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgt
    18001 tggccgccgt acagggcaac cacgtttacg ccgcgcatgt gtttagagaa atccgtcatt
    18061 gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct
    18121 ttcagctcag gatcaagatt ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc
    18181 cccgtctggg ccatacccag aacgtcgcgg ccattcagca gatgtggaat acactctgcc
    18241 tgaattggag atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc
    18301 aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt
    18361 aatggcggcc agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg
    18421 tgaaccggct caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg
    18481 gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca
    18541 ggtcatcttg gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa
    18601 agttatgaac gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc
    18661 ccagacttag gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg
    18721 aggtgttatc cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc
    18781 ccaggtagaa ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct
    18841 gtttcaacac ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca
    18901 gactacggaa aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta
    18961 acttgtcacg accgccgtaa tataatgcga tcccgcgatt caagtgcgcg tagttgtaag
    19021 ttggatcaag ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct
    19081 gcgttaaata tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg
    19141 cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc
    19201 gctcatataa aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt
    19261 ccatacgtgc cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt
    19321 cacttttacg ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac
    19381 accagcgcaa aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg
    19441 ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca
    19501 aatggcaacc ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag
    19561 actgctcagt cgcttcttta atgctcagac ggatacggcc ctggcgatca acttccagaa
    19621 ctttcaccgg tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttat
    19681 cagcgatttg agagatgtgg accagacctt ctttaccgcc gccgatggca acaaatgcgc
    19741 caaagtcaac gattcgggtc actttaccat tgtagacgcg gcctacttcg atttctgcag
    19801 tgatctcttc gatacgacga atagcgtttt tcgccttctc gccgtcggtc gctgcgatct
    19861 tcacagtacc gtcatcttcg atttcgatgg tagtgccagt ttcttcggtc agagcacgga
    19921 ttacagaacc gcctttaccg ataacgtctt tgatcttgtc tggattgatc ttgatggtat
    19981 ggatacgcgg agcgaactca gagatatcac cgcgcggcgc attgatcgcc tgttccatta
    20041 cgcccaggat atgcagacgc gcacctttag cctggttcag tgcaacctgc atgatctctt
    20101 tggtgatacc ttcaattttg atatccatct gcaatgcaga gataccgtca cgagaacccg
    20161 caactttgaa gtccatatcg cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa
    20221 cgtagttgtc gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgatcg
    20281 gcacaccggc gtccatcagc gccagagacg cgccgcacac ggaagccata gaagaggaac
    20341 cgttggattc ggtgatttca gacacaacac gtacggtgta cgggaatttg tccatatccg
    20401 gcattactgc cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg
    20461 gagaaccgac catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga
    20521 aggtatcggt acgttcgccc atcagttcat caagaacctg cgcgtcacga gcagtaccca
    20581 gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac
    20641 gcggcagcac accagtacgc acatccagac cacggatcat atctttttca cgaccgtcga
    20701 tacgcggttc gccggccagt acgcggctac gaacaacatt tttctcgata gcgtgcagaa
    20761 tttcacccag ttcgttttcg tccagggttt cgtcttcagc aagcagtgtc gcgatggttt
    20821 cagatttgat gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat
    20881 cgctcagacg agcttcagcc agcgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct
    20941 ccggctgcca atcccaacgc ggtttaccgg cttctttcac cagttcattg atgttctgaa
    21001 taacaacctg ctgttgttca tgaccgaaca ccactgcgcc cagcatttgg tcttcgctca
    21061 gcagttcagc ttcagattca accatcagca cggcagcttc tgtaccggca acaaccaggt
    21121 ccagtttgct ctctttcagt tcgtcctgag tcgggttcag tacgtactgg tcattgatgt
    21181 aacctacacg ggcagcacca atcgggccat tgaacggaat accagacaga gacagtgctg
    21241 cggaagcacc aatcatcgcg acgatatccg ggttaacctg cgggttaaca gaaaccacgg
    21301 tggcgataac ctgaacttcg ttgacgaagc cttccgggaa cagcgggcga atcgggcggt
    21361 caatcagacg cgcgatcagg gtttcgcctt cgcttgggcg gccttcacga cggaagaagc
    21421 tacccgggat acgaccagca gcgtaggtac gctcctgata gttaacggtc agcgggaaga
    21481 agtcctgacc tggtttggct tttttctgac caacaacggt aacaaatacc gcggtgtcat
    21541 ccatgctaac cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca
    21601 cggtgtgttg gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc
    21661 tctttcttag acagtacctt acggtactgg tgttaatacc cgatcttctg cgcatcctcg
    21721 cgactaatga caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa
    21781 cctctgcaac ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg
    21841 tcagcttgaa aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac
    21901 gcagacccag gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt
    21961 cgagcagttt acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt
    22021 ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct
    22081 gaacttcggt agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg
    22141 ttgcttcagt acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga
    22201 tctctaattc agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt
    22261 aagcggcagt attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg
    22321 tattcaacca ccaggcgacg aggcgcaacg cggccttcat cgtcaatttc gcccataccg
    22381 ataaacttgc cgttctcacc ctctgtaacg cgaaccagtc cttccagcgg cgcaccggat
    22441 gtacgaactg ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccact
    22501 gggtagtccg aagctggact gtccattggc atcagtaatg ggtcaagtaa ctccgcagcc
    22561 ggaatatcct gctgttcggc ttgctcaacc agttcacgca gatgttccag ggtcaccatc
    22621 cgttcaaccg gatatttact taccgccaga cgacgcaagt aaataacatg cgcgccacaa
    22681 ccgagttttt cgccgagatc gtcaatgata gtgcggatat aagtgccttt tgagcagtga
    22741 atttccagct ccagctcatt gccttcatgg cgaataaaca gcaattcata aacggtaatc
    22801 gggcgcgctt cacgtggaac ttcaatgccc tgacgcgcgt attcgtacag ttttttgccc
    22861 tgatatttca gtgctgaata catcgaagga atctgttcga tatcgccacg gaaggtatcc
    22921 agcgccgccg ccagttgctc tgcactaaag gttaccggac gttcttcaac gatttgtccg
    22981 tcggcatcag aagtatcggt acgctgtcca agacgcgcaa tgacccgata acgtttgtcg
    23041 gagtccagca gatactggga aaacttcgtc gcttccccga ggcaaattgg caacatgccg
    23101 gtcgccagcg ggtccagcgc accggtatgc ccggcacggt tggcgttata aatccgtttc
    23161 actttttgca gcgcatcgtt gctggacatc ccctgcggtt tatccagcaa caaaacgccg
    23221 ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt
    23281 taacacgacg ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc
    23341 cttcaaccag agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg
    23401 ctttccccag caggctgcgg atgaaaccag aagcttcctg caacgctttg atgcccgctt
    23461 taaccgcgtc ttcatctttg tcgttgagga acgtcacata tactttggca tacgccaggt
    23521 cgcgagacat ttcgacaccg gaaacggtgg tcatcatgcc cagacgagga tctttaattt
    23581 cacgctgcag gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac
    23641 caaattcttt cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa
    23701 aattaatttc aggtggaagg gctgttcacg ttggcctgat aagacgcgcc agcgtcgcat
    23761 caggcaatcc atacaggatg cggcataaac gccttatccc gcatggaacc ctaaaaacct
    23821 taagcgatgg tacgttggat ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg
    23881 acatcgttgt agttcttaac gccgatacca cattccatac cgttacggac ttcgttaacg
    23941 tcatctttga agcgtcgcag ggactccagc tcgccttcgt agataaccac gttgtcgcgc
    24001 agaacgcgga tcgggttgtg acgtttaacc acaccttcgg taaccataca acctgcgata
    24061 gcaccaaatt tcggtgattt gaacacgtca cgaacttccg ccagaccgat aatctgctgt
    24121 ttcagttccg gagacagcat accgctcatc gccgctttca cttcgtcgat caggttatag
    24181 atgacggagt agtaacgcag atccaggctt tccgcttcaa tcactttacg tgcagaggca
    24241 tcagcacgta cgttgaagcc aaccaggatg gcgttggacg ccgcagccag ggtagcgtcg
    24301 gtttcggtga taccacctac gccagaaccg atgatcttca ctttaacttc gtcagtagac
    24361 agtttcagca aggagtcaga aattgcttcc acagaaccct gtacgtctgc cttcaggacg
    24421 atattcactt cgtgaacttc gccttcggtc atgttggcga acatgttctc gagtttagat
    24481 ttctgctgac gcgccagttt aacttcgcgg aatttaccct gacgataaag tgcaacttca
    24541 cgcgctttct tctcgtcacg tacaacggta acttcatcac ccgcagccgg tacgccggac
    24601 aggccgagga tttccaccgg aatggacgga cccgcttcca gcacttcctg acccagttcg
    24661 ttacgcattg cacgaacacg accgtattcg aagccacaca gaacgatatc gcccttgtgc
    24721 agagtacctt cacgtaccag aacggtagca accggaccac gacctttatc gaggaaggat
    24781 tcgataaccg caccgctcgc cataccttta cgtaccgctt tcagctccag aacttccgcc
    24841 tgcagcagga tagcatccag cagttcatca ataccggtac ccgcttttgc agatacgtgt
    24901 acgaactggc tttcgccgcc ccactcttct ggcaggatgc cgtactggga gagttcgttc
    24961 ttaacgcgat ccggatcggc ttctggttta tcgattttgt tcacagcaac caccaccgga
    25021 acacccgccg ctttcgcgtg ctggatagct tcgatggtct gcggcattac accgtcgtct
    25081 gcagcaacaa ccaggactac gatgtccgtt gcctgcgcac cacgtgcacg cattgaagta
    25141 aacgcggcgt gccccggggt gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg
    25201 tatgcaccaa tgtgctgggt aatgccgccc gcttcgccag aggccacttt cgttgaacga
    25261 atgtagtcca gcagagaggt tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc
    25321 gcgcgcggtt cagccgcagc acccgtgtca cggtcgctca ttaccgcctc ttccagctcg
    25381 ttttcacgac gcaggataac tttgtggccc atctcttcag caaccagctg tgcggtttcc
    25441 tgatcgataa cctggttgat ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc
    25501 tgagagcctt taaccgccat cttgttcgcc agttcgccaa cggtgatggt ttcgccgatc
    25561 acaacgtcac ggttaacggc ctgagctggc ttctggaagc cttgctgcag cgaagaacct
    25621 ttacgttttc cgcccttacc gccacgtact gctgcacgag cttcttcacg atcagctttt
    25681 gattcagcgt gtttgttgcc tttcttcgga cgcgctgctt tcgcgttacg accacggcca
    25741 cggccgcctt cgacttcacg atcgctttcg tcttctgcct ggcgagcatg ttgagaagta
    25801 gtgacgtgat aatcgctgga atcttcagtc ggttccgcgt tatcagtcca tttgttttct
    25861 tccgccatac gacgtgcttc ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc
    25921 gcttcttctt cggctttacg cttgagctct gcagcttcct gctcacggcg ggctttttca
    25981 gcctgggcgt ttttagtcat atcgtcttgt tgattgctca ctttgtcttt ttccgcagct
    26041 tcacgtttcg cttgttcagc agcttcacgc ttagcttgtt ctgcggcctc acgttcagct
    26101 ttttgttgcg cctcgcgttt agccgattct tctgcctcac gacgggcttg ctcttccgct
    26161 tcacgctgcg cttgctcttc cgctgcaagg cgctcagcct cttgcggatc gcgtttcaca
    26221 aaggtgcgtt tcttgcggac ttcgatttgt accgatttgc tttttccacc ggtaccagga
    26281 atgttaaggg tgctgcgtgt tttacgttgc agcgtcaatt tgtccgggcc tgaatttttc
    26341 tgattcaggt ggtcaatcaa agtctgtttc tcttgtgcag acacagagtc gtcagcagac
    26401 ttccggatac ctgcatcagc aaattgctgt accaggcgtt ccacggaggt ctgtcgctct
    26461 gcggccagcg ttttaatcgt tacatctgtc atgctgttcc ttcctgctac agtttattac
    26521 gcttcgtcac cgaaccagca aatattacgg gcagccataa tcagtgctcc ggctttttcg
    26581 tcggtcaacc cttcgatatc agccagatca tcaatgccct gttcggcgag atcttccagc
    26641 gtacaaacgc cacgggcggc cagtttgaat gccaaatcac gatctacccc ttcaaggttc
    26701 agcagatcat cagccggttt gttatcaccg aggctttctt cctgggcctg tgcaatggtg
    26761 gccagtgcat ttttagcacg ctcgcgcagt gcttcaacgg tcggctcatc aaggccttcg
    26821 atttccaaca gctctttcat cggcacatag gccagttctt ccagcgtcga gaagccttct
    26881 tctaccagaa cagtcgcgaa gtcttcatcg atgtcgagat atttggtgaa ggtgtcgatc
    26941 gctgcgtgcg cttccgcctg atgcttagcc tgcaggtcgt caacggtcat cacgttgagt
    27001 tcccaaccgc tcagctgcga agccagacgc acgttctgac cgttacggcc aatcgcctgc
    27061 gccaggttac cggcttcaac ggcgatatcc atggtgtgtt tatcttcatc caccacgata
    27121 gaagcaacgt ctgccggtgc cattgcgtta atcacgaact gcgccgggtt atcatcccac
    27181 aggacgatat cgatacgctc tccgcccagt tcagtagaaa ccgcctgaac acgcgcgcca
    27241 cgcataccta cgcaagcacc taccggatcg atacgtttat cgttggtttt caccgcaatt
    27301 ttcgcacgag atcccgggtc gcgagccgct gctttaattt caatcacttc ttcgccgatt
    27361 tccggcactt caatacggaa cagttcgatc agcatttccg gcttggagcg ggtgacgaac
    27421 agctgcgcgc cacgcgcttc cgggcgaacg gaatagagca cgccacgaac gcggtcgcca
    27481 gggcggaagt tttcacgcgg cagcatatct tcgcgcagga tcacggcttc agcgttgttg
    27541 cccagatcca gagagatgtt gtcgcggttt acttttttca ccacgccggt gatgatttca
    27601 ccttcgtgtt cacggaactg atcaaccacc atcgcacgtt cggcttcacg cactttctgc
    27661 acgataacct gttttgccgt ctgggtagtg atacgatcaa aggtaacaga ctcaatctga
    27721 tcttcaacgt aatcgcccaa gttcaggctt tcatcttcat aacgtgcggc ttcaagggtg
    27781 atttccttgg tcggctgggt gacttcatca acaactaacc agcgacggaa ggtgtcaaaa
    27841 tcaccgcttt tgcgatcgat ctgtacgcga acgtcgattt cttgttcata ttttttcttt
    27901 gttgctgtcg ccagcgcgct ttccaatgct tcgaaaatct tctcgcgagg tagcgccttt
    27961 tcattggata cggcttcaac tacagccaaa atttctttgt tcatcgcggg cttttcacct
    28021 catccagact attaaaagtg gggaaccagg ttcgccttct ggatattact cagcgcgaac
    28081 acttcatctt taccttcgac ggtaactgtg atcatttcac cgtctaccgc tttgataacg
    28141 ccctgccatt tacgacggtt ttgtaccgcc atacggagaa ccagagtcac ctcttctccg
    28201 acaaaacggg cgtagtgttc agccgtgaac agtgggcgat cgagacccgg tgaggagact
    28261 tccaggttat aagcaacggt gatggggtct tcaacatcca gcacagcact tacctggtgg
    28321 ctcacatcag cacaatcatc aacattgatg ccatcttcac tatcaatata gatgcgcagt
    28381 gtggatgtgc gaccgcgaat aaattcgatg ccaaccagtt caaaacccag ggcctcaact
    28441 ggcgcagtaa tcatctctgt taatttttgc tctaatgtgg acaagcccac ccccaagaca
    28501 taaaaaaagg gcctaaagcc cagttattct gtagtcagat aacaaaaaac cccgataaat
    28561 cggggcttta tataactgaa ccctataacc gcaactgcgg tctggagcac tttccagaag
    28621 gattttttca aatcccacta cgaaggccga agtcttcaca gtatatttga aaaaggactc
    28681 taagggaaag tggttgcggg ggccggattt gaaccgacga ccttcgggtt atgagcccga
    28741 cgagctacca ggctgctcca ccccgcgcct gaaacgtggc aaattctact cgttttgggt
    28801 aaaaaatgca aatactgctg ggatttggtg taccgagacg ggacgtaaaa tctgcgggca
    28861 ttatagtgat ccacgccaca ttttgtcaac gtttattgct aatcacgtga atgaatatcc
    28921 agttcacttt catttgttga atacttttac cttctcctgc tttcccttaa gcgcattatt
    28981 ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa tgaaaactca
    29041 tttattttgc ataaaaattc agtgagagca gaaatccagg ctcatcatca gttaattaag
    29101 cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa cgtattggta
    29161 tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga caaaagggag
    29221 ctgttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat tatgatgcga
    29281 tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac tgccgcaaac
    29341 aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac accactggtg
    29401 gactgaccta tttcaacacg acgccgctgg gccgcgccgt gaccggcacc atgctggttg
    29461 ctgctatgaa agaagatggc gtgaatatct ggggtgacgg cagcacctat aaaggaaacg
    29521 atatcgaacg tttctaccgt tacggtctgc tgaccaatgc tgaactgcag atttacaaac
    29581 cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg tctgaattta
    29641 tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcttactcc acggactcca
    29701 acatgcttgg tgcaacgcat gaagcgaagg atctggaata cctcaactcc agcgtcaaaa
    29761 tcgtcaaccc aattatgggc gtgaagtttt gggatgagag cgtgaaaatc ccggcagaag
    29821 aagtcacagt acgctttgag caaggtcatc cggtggcgct gaacggtaaa acctttagcg
    29881 acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc ctgggcatga
    29941 gcgaccagat tgaaaaccgt atcatcgaag caaaaagccg cggcatttat gaagcccctg
    30001 ggatggcact gctgcacatt gcgtatgaac gtttgttgac cggtattcac aacgaagaca
    30061 ccattgagca gtatcacgcg catggtcgtc agttgggccg tctgctgtac cagggacgtt
    30121 ggtttgactc ccaggcgctg atgctgcggg actctctgca acgctgggtt gccagccaga
    30181 tcactggtga agttactctg gagctgcgcc gcgggaacga ttattcaatc ctgaataccg
    30241 tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc gactcggtgt
    30301 tctcgccaga tgatcgtatc ggtcaactga ccatgcgtaa cctggatatc accgataccc
    30361 gcgagaaact tttcggttat gccaaaactg gcctgctctc ctcctctgcc gcttcagggg
    30421 tgccgcaggt ggagaatctg gaaaataaag gccagtaatt cgacatcaac cctgcccttc
    30481 aggggtgggg ttaccttgat tatcctctgt aaactccttt ctgagcatca cataatcgaa
    30541 aacatcatgg tttgcatcca caacccgcga gtctccttta tagtgtgcaa tcacttcctc
    30601 aagcgtttgc ggctgttccg gctttgttac ccccatccac gcattaataa tgtaattatt
    30661 gtaagctgtc gaaaagatgt tgttttccgt tgtgcgatcc acgccatccc caagaacggg
    30721 gctataccag ataaacatag ggacatgata tgcctgctgg ctagcctccc tgcctccatg
    30781 aaaatagacg ttcttcttcg tagggtcgcg ttccaggccg tggtcggcaa aatacatgac
    30841 cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat ctgtgtaatg
    30901 aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacag cgctggattg
    30961 cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa tcagtttctt
    31021 ttgctgcgtc ttttgctgta acgcttgcga taaatgcggc aacaacaatt catcaaatcc
    31081 tctgacatag actgtttcca tggcgcgcat ggcgatactg gtaactgctg taccattctg
    31141 ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat tagccatatt
    31201 aataatgttg tccggataat tatgaatgtc atgactcaaa acagaatcag cagttaaaga
    31261 aaggggaacc gacagcgcag tgtaaggtgc accgcttatt gcttgattaa acagtttgat
    31321 ctgctttctt tgtgcttcga cttgcggtgt cgtagagtgt gtatatccat acaaagacat
    31381 attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac ctgtatccct
    31441 gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt gatgctcttt
    31501 cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa atcgcgacgc
    31561 tagtatatat ggactgaacg cctctttatt ttttgcatct ttataagcaa attgacaagc
    31621 ggaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt tttttgtcgg
    31681 caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa aggcaaaagc
    31741 acatagataa gggatataca ttcccagcat tttgacaact tcatccggat cactctgcag
    31801 cacactaatc gcaaatccat cattgaaagt tgtaccaaag atacaccatg aatagaggct
    31861 aatactcata tctgccgcag tcagtaacac aaatggtgcg gcaataagca ggcgaagcgg
    31921 aagggatggt atacgtttaa caaccaggat ggtcagaacg aagaacagca atcgctgaac
    31981 catatgcgct ccggaagaag cgactaagtt cgtaatacca aaaataataa cacaaagata
    32041 caacaaccaa tgagatttaa aacttttaac aaatttgttg aatactgtca tccagaaatc
    32101 caagttcaca accaatataa acaattatca acaatgttca attttatatc agacatgttt
    32161 ttcaactcta atccatatca gaccgaaaca ctgctagatt ttttcttttt aaatgatgcg
    32221 gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca ttacgcagaa
    32281 cgtagccatc agtagttata ctgacagggg caggatggtt tatcgagaag taaacatatc
    32341 cgtattcgta ccggaaatgg agttgacaca ataaagtgcc aattatgtca gcagaaggga
    32401 aaagcttata actaaggcat accggcacca tcgtttcaag gtaccagcta cgagtaaaac
    32461 aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa aacgaaaaaa
    32521 gacgcttttc agcgtctttc tggaatttgg taccgaggac gggacttgaa cccgtaagcc
    32581 ctattgggca ctaccacctc aaggtagcgt gtctaccaat tccaccacct cggcacggat
    32641 actacttttt agttcgggat atcgctggtc ggcttagccg gagcagctgg ctgagtttgt
    32701 tcggttttcg ccggcgcact caggttttcc cattcgctac ctttattggt tttgttgcta
    32761 ttgatgttac ccagcaccag actgatgatg aagaataacg ttgccagcag cgccgtcatg
    32821 cgagtcatga agttaccaga accacttgaa ccaaacagcg tagcggaagc gcctgctccg
    32881 aaggaggctc ccatatcagc gcctttacct tgctgcagca tgatcagacc aacaaggcca
    32941 attgccacaa taaggaaaac tactaaaaga gcttcgtaca taatcaacct gttccttgcg
    33001 gagttgccgc gtaccaatgc ttcaaccaat aaagcgggaa tatttaacct ttcccactga
    33061 agcgggtgtg aatactaacc aaagcgaatg accttcgcaa gggcaatttt attgcattgt
    33121 atcaactgcg gaaaaaaaca gcaaaaccga tgtgttcgct gagaaaaaag gcgacaatcg
    33181 ccgccttttt agccagccat ttatcgtttt aaacggcttt tactgcatcg gcgatgcggt
    33241 gtgcaaattc agtcacctgc gcttcgtctt cgccttccac catcacgcga attaacggtt
    33301 cggtgccgga tttacgcaac agcacacgtc cacggctacc cagcgccgct tcaacttctg
    33361 cggtcacagc tttgactgat tcatgctcca acggatcgcc gctacctgcg gtgtaacgca
    33421 cattaaccag aatctgcggg aacattttca tgccgctgca aaggtcgtgc aggctcatat
    33481 ggttacgcgc catcgccgcc agcacctgca agccagcaac aatgccgtca ccggtagtgg
    33541 ttttatccag caggatcaca tgaccggagt tctctgcacc gatacgccag cctttctcct
    33601 gcattttttc cagtacgtag cggtcaccca ctttcgcgcg cgcaaatgga atacctaact
    33661 gtttcagcgc cagttcaagc cccatgttgc tcatcaatgt acccacagca ccaccacgca
    33721 gctggccctg acgaagacct tcacgcgcga tgatatacat gatctgatcg ccatcgactt
    33781 tattgccttc atggtcaacc ataatcacgc gatcgccgtc gccgtcgaag gcaataccga
    33841 gatccgcttt ttcagccagc acacgagcct ggagcgcgcg aacgtcggta gcccccactt
    33901 cggcattgat atttacaccg ttcggctcac aaccgatagc gataacgttc gcccccagtt
    33961 cacgcagcac gttcggtgcg atgtgataag tcgcaccgtt tgcacaatcc accacaatct
    34021 tcagttcact gaggctaagt tcgttcggga acgtggcttt gcaaaactcg atataacgac
    34081 ccgcggcatc aacgatacgg ctggctttac ccagttctgc cgaatcaaca cagctgatct
    34141 ctttttccat ttccgcttcg atggcttctt caactgcatc cggcagctta gtaccgtcaa
    34201 tagaaaagaa tttaatgccg ttatcgtaga atgggttatg cgatgcagaa attacaattc
    34261 cagcctctgc gcggaaggta cgcgtcagat aagccacggc cggtgttggc atcgggccag
    34321 tgaagagcgc ggaaagaccg gcagccgcaa gacccgcttc cagtgctgac tccagcatat
    34381 agccagaaat acgcgtgtct ttaccaataa taatcttacg agagccgtgg cgcgccagca
    34441 ctttacccgc ggcccaaccc agcttaagca caaaatcagg tgtgatcggc gcatccccta
    34501 cacgaccacg aatcccatcg gtaccgaaat atttacgatt actcatagcg tttgttttcc
    34561 tttgcagaca gagtggcttc caccacccgc atcgcttcta cggtttcttt gacgtcatga
    34621 acacgaatga tgtgcgcgcc ttgcattgcg gcaatgaccg cacaggccag actaccgctc
    34681 aggcgctcgg acggccccac gttcagcaat tgcccaatca tcgattttcg tgacataccc
    34741 accaacagcg gcaggttgaa atggtgaaat tcagccaggc gcgccagtaa tgaatagtta
    34801 tgggagagat ttttaccgaa accgaatccg gggtcgagca acaatttctc ttttgcgata
    34861 cccgcctgct cgcaacgtgc tatttgctca ataaagtagc gattcacttc tgcaaagaca
    34921 tcgtcatact tcggcgcttc ctgcatggtt tttggatttc cctgcatatg catcagacaa
    34981 accggtaaac cggtttctgc agccgcctcc agagcaccag gttcggaaag ggagcggata
    35041 tcgttaataa tgtgagcgcc aactttcgct gactcacgga tgacttctgg tttggatgta
    35101 tctaccgaga tccagacttc gaagcgttga gcaattgcct caaccacagg aataacacgt
    35161 tgcaactctt cttcaacgca aacttccgcc gcccctgggc gcgtggactc gccaccaacg
    35221 tcaatgatcg tcgcgccagc gttgatcatc agattcgcat gtttcaccgc atctaacagc
    35281 gagttatgcg tgccaccatc cgaaaaggaa tcaggcgtga cgttgaggat ccccattacg
    35341 tgaggatggc taaggtccag tgaagtaccc tgggcaaaga gtttcatgat gttatccctg
    35401 gtatgaattg ataagaaaaa accccggagc acgccccggg gttttcggta caaatacagt
    35461 catctgatac gggaacttac ttgtcgccta actgctctga catggtgtta cccgggttcg
    35521 gcgtacgcgg ttcatcaacc ggacgaggag cctttggact accattgtcg ccagaattgt
    35581 tagaagcgcc tggttcttcc cagcccgctg gcggacgtac atcgcgacgt gccatcaggt
    35641 catcaatctg cggtgcgtcg atagtctcat atttcatgag agcatctttc atcgcatgca
    35701 gaatatccat attgtcggtc agaagctgac gcgcacgatt atagttacgc tcaatcagtg
    35761 ctttcacttc ctggtcgatg atacgtgcag tttcatcgga catatgtttc gctttcgcta
    35821 cgctacggcc gaggaacact tcaccttctt cttccgcgta cagcagtgga cccaatttct
    35881 cagagaagcc ccactgggtc accatgttac gtgccaggtt ggtcgcaact ttaatatcgt
    35941 tggacgcccc ggtagaaaca tgttccggtc cgtagatgat ctcttccgcc agacgaccac
    36001 cgtacagcgt ggagatctga ctttccagtt tctgacggct ggcgctgatt gcgtcgccct
    36061 caggcaagaa gaaagtcaca cccagcgcac gaccgcgtgg gataatcgtc actttgtgca
    36121 ccggatcgtg ttccggcacc aggcgaccga taatcgcatg acccgcctcg tggtaagccg
    36181 tcgattcttt ctgcgcttcc gtcatcacca tggagcgacg ttccgcaccc atcatgattt
    36241 tgtctttcgc tttctcgaac tcaaccatcg aaacaacgcg tttgttgcca cgagcagcga
    36301 acagtgccgc ttcgttcacc aggttcgcca ggtcagcacc ggagaaacca ggagtaccac
    36361 gggcaatgat tgccgcgtcg atatcgggtg ccaatggtac gcgacgcatg tgaactttca
    36421 ggatctgctc acgaccgcga acatctggca agccaaccac aacctgacgg tcgaaacggc
    36481 caggacgcag cagggctggg tcgagaacgt ccggacggtt agtcgcagcg ataacgatga
    36541 taccttcgtt accttcaaaa ccatccatct caaccagcat ctggttcaga gtctgttcac
    36601 gttcatcgtg accaccgccc aagcctgcgc cgcgctggcg gcctacggcg tcgatttcat
    36661 cgataaagat gatgcacggt gccgctttct tcgcctgttc gaacatgtca cgaacacggg
    36721 atgcacccac accgacgaac atttctacga agtcagaacc ggagatagta aagaacggaa
    36781 ctttcgcttc gcctgcaatt gctttcgcca gcaacgtttt accggtaccc ggaggaccta
    36841 ccatcaggac gcctttcggg atcttaccgc cgagtttctg gaagcggctc ggctcacgca
    36901 gatactcaac cagttcagca acttcttctt ttgcttcgtc gcagcccgca acgtcagcaa
    36961 aggtggtttt gatctgatct tccgtcagca tgcgcgcttt gctcttacca aacgacatgg
    37021 cacctttgcc accgccgccc tgcatttgac gcatgaagaa gatccagaca ccaatcagca
    37081 acagcatcgg gaaccaggag atgaagatag aagccagcag gcttggttct tcaggcggtt
    37141 caccgacaac cttgacgttc ttggtcaaca ggttatccag taatttcgga tcctgaaccg
    37201 gaatgtaagt ggtataacgg ttactatctt tcttggtaac gttgatttca cgtccgttga
    37261 tacgcgcttc acgaacctgg tcgttattga cctcttgtag gaaggtagag taatccacct
    37321 tacggccatt agactcgctg ggcccaaagc tctggaatac tgacatcagc acaacggcaa
    37381 tgaccagcca gagtattagg tttttcgcca tgtcactcaa gggattaacc tcttattaca
    37441 actgtgttaa aaacagcgtc aggatactct atatccagca tctttcaaac tttcgtctga
    37501 aatctcccgg ttagggttta cgcccggtcg ctacaatata cacttcccgc gaacgtgcac
    37561 gagaagagtc cggcttacga actttgacct tcgtaaacag ggagcgaatt tcccttagat
    37621 actcatcgaa accttcgccc tggaacacct tcactacaaa actgccacct ggcgctaata
    37681 catcacgaca catttctagc gccagttcca ccagatacat ggcacggggg atatccaccg
    37741 ccggtgttcc gctcatgttt ggtgccatat cggacatgac aacctggact ttgctgtcgc
    37801 caacgcgctc cagcagtgct ttcatcacca gttcatcacg aaaatcgccc tgaagaaagt
    37861 ccacaccaac gataggatcc ataggtaaaa gatcgcaagc gatgatgcgg cctttgccgc
    37921 caatttgggt gaccacatat tgtgaccaac cacccggagc agcaccaagg tcgacaaccg
    37981 tcattcccgg tttaaagagt ttgtcacttt gctgtatttc atcaagttta aaccaggcac
    38041 gggaacgtaa cccctttttc tgtgcctgtt gaacatattt atcgctaaag tgttcctgaa
    38101 gccagcggct ggagctggca gaacgcttct tacctgtcat ttaactttcc catggggata
    38161 actcatcgta accaattgcg taaattttta cgcgcctatt tggtgatata tgggagatgg
    38221 cggtagaatg acccgttttc aatcccaacg taagcaaaaa atacgatgaa tctgagtact
    38281 aaacaaaaac agcacctgaa aggtctggca catccgctca agccagttgt tctgcttggc
    38341 agtaatggtt tgaccgaagg ggtgctggcc gagattgaac aagcgttaga gcaccatgaa
    38401 ctcatcaagg tgaaaatcgc caccgaagat cgcgaaacta aaaccttgat cgtggaagct
    38461 atcgtgcgcg aaaccggcgc ctgtaatgta caggtcatcg gtaaaacgct ggtgctttat
    38521 cgcccaacta aagaacgtaa aatctcgctg ccacgctaag attatcctaa agttacacac
    38581 attcgctgtg taaaacgagg ggttttccgc aggcaggaga gcaaaatgcc acgctctgtt
    38641 cgttgataaa aggccgcata gcggcctttt tcctttcttt acaatacatc aacatcttga
    38701 gtattgggta attcttacag gtattccacc ttaattactt caaattctac ttcgccgccc
    38761 ggcgttttga tgaccacaac atcatcttct tctttgccga tcaggccacg agcaataggc
    38821 gagtttacag aaatcaggtt ttgtttaaag tcagcttcgt catcgccaac gatgcgataa
    38881 gtctgttctt cgtcagaatc gagattcagc accgttacgg tagcaccaaa aataacgcgc
    38941 ccattgttgg gcattttggt gacatcaatc acctgcgcgt tcgacagctt ggcttcgatg
    39001 tctttgatac ggccttcgca gaagccctgc tgttcacgag ctgcgtggta ttcggcgttt
    39061 tctttcaggt cgccatgctc acgcgcttcc gcgatagcag caatgatttc aggacggcgc
    39121 acagatttca gaaaatccag ctcttcgcgt aatttttcag cgccgcgtaa ggtcatcgga
    39181 atagcttgca tttgttatac ctcttgaata ttcctgatgg ggcaagtctt cacccacccc
    39241 ggctgttcgg cctgcccggc ataactcctc accgggacca gaagcaaaaa aataccgacc
    39301 cgggtacaag tcccaggtca gctacaattc acattttgat agtcatttta ccctggagtt
    39361 cccgaagggt catcgtttac tttatagggc gttgcgccgt agtatgacgg ctcgattcca
    39421 ggttgttagc gcgagattat gcgattttcc agatttatca tcggattgac cagctgtata
    39481 gcgttcagtg ttcaggccgc aaatgttgat gagtacatta ctcaactccc cgcaggtgcc
    39541 aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc ccgctattga ttaccacagt
    39601 cagcagatgg cgctacccgc cagtacgcag aaagtgatta ctgcgctggc ggcgttgatt
    39661 caactcggcc ccgattttcg ttttaccacg acgcttgaaa ccaaaggcaa tgtggaaaac
    39721 ggcgtactta agggtgactt agttgcgcga tttggtgccg atccgacgtt aaaacgtcag
    39781 gatattcgca atatggtcac gactttgaaa aaatctggcg tcaaccaaat cgatggcaat
    39841 gtgttgatag atacctccat tttcgccagc cacgataaag cccccggctg gccatggaat
    39901 gacatgacac aatgctttag cgcaccgcct gccgccgcca tagttgaccg caactgtttc
    39961 tccgtctcgc tctacagtgc cccaaaacct ggtgatatgg cttttatacg cgtggcatct
    40021 tattaccccg ttacgatgtt cagccaggta cgcaccctcc cccgtggttc tgccgaagcg
    40081 caatactgcg aactggatgt cgtaccaggt gacctgaacc gctttacgct aacgggatgc
    40141 ctgccacaac gttctgagcc gctcccgttg gcttttgccg tgcaggatgg tgccagctat
    40201 gccggggcaa ttctgaaaga tgagttaaaa caggcgggta tcgcctggag cggaacactg
    40261 ctgcgccaga ctcaggttaa cgaacctgga acggtagttg ccagtaaaca gtcggccccg
    40321 ctgcacgatc tgcttaagat tatgctgaaa aagtcggaca acatgattgc cgatacggtt
    40381 ttccgcatga tcggccatgc gcgcttcaat gtgcctggaa catggcgggc cggatcggac
    40441 gccgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata tcggaaacac cattattgcc
    40501 gatggttcag ggctttcgcg gcataacctg attgcccccg ccaccatgat gcaggtgctg
    40561 caatatattg cccaacacga caatgaactt aactttatct ccatgctgcc gctggcgggc
    40621 tatgacggct ctttgcagta ccgtgcaggt ctgcatcagg cgggcgtgga tggaaaagtc
    40681 tcagcgaaaa ccggttcgtt gcagggggta tataacctgg cgggattcat taccacagcg
    40741 agcgggcaac gaatggcgtt tgtgcaatat ctttctggct atgcagtaga accggcggat
    40801 cagcgtaatc gccgtattcc gttagtgcgt tttgaaagcc gtttgtataa agatatttat
    40861 cagaataatt agcctaaaga gaaaccccgg cacatggccg gggttttgct gattaacgct
    40921 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc cagtcctcat
    40981 catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac atgaattcga
    41041 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    41101 tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc agataatatt
    41161 tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct tctgccttat
    41221 ccagcagatc gatcttgttg aaaactaacc aacgcggttt cgccgccaga tcctggctgt
    41281 atttttccag ctcactgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
    41341 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgaa
    41401 tgcccagacc tgcgccttct gcagcgcctt caatcagccc tggaatatcg gcaacaacga
    41461 agctcttttc gttgtccatt cgcaccacgc ccaggcttgg taccagagtg gtaaacggat
    41521 aatccgccac tttcggttta gccgccgata cagcacgaat aaaggtcgat ttacccgcgt
    41581 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    41641 tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac
    41701 gagtgttgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttgg
    41761 tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    41821 taatcgtgac gtctttaccg cgcttacctg tacagtcgcg gcttgcaccg ttttgaccgc
    41881 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg
    41941 cttccatcca tacgtcacca ccgtcaccgc cgtcgccgcc atccgggccg cctttcggaa
    42001 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    42061 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    42121 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    42181 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    42241 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    42301 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    42361 gggcgccata tcctaccaat gtattcagtc cgcaaaaaat taaacaggcg agctgccagt
    42421 ggctaagctg cgctatcact ccaggatttg ccagagggaa gagcgcaatt gtacataaag
    42481 tgtacagcaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    42541 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc caggtgtaat
    42601 cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca
    42661 ggagcatcaa tgcccctaca acctgagtac tgcgcatttt ctctttcagg ataaatacgc
    42721 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    42781 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    42841 ctaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac cgcttcttca
    42901 ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    42961 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    43021 ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    43081 aaagcgcata tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa
    43141 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    43201 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    43261 ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    43321 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcacca ccgaaacgct
    43381 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    43441 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    43501 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    43561 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    43621 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    43681 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    43741 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    43801 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    43861 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact
    43921 tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat
    43981 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    44041 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    44101 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    44161 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    44221 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    44281 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    44341 gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    44401 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agccgtaaac
    44461 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    44521 gaaaactaca tgcgcgttat ctacagcaag actgcgcgtc tgtttgaggc tgccgcgcag
    44581 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctaca agattatggg
    44641 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    44701 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    44761 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgattcgtac cgccatcgaa
    44821 cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    44881 cttgaatgga cgcgtcagcg tgccgaggaa gaagcagaca aagccatcgc agcgttacag
    44941 gtgctcccgg acaccccgtg gagagaagca cttatcggcc tcgcgcacat cgctgttcaa
    45001 cgcgatcgtt aatcccctcc cctcatcccg cgcagtgcgc gggatgattc cagtaaattc
    45061 cataaaacac ttattcagct ctataactcc tgaaaaacag ggctgtgtat ctttatatat
    45121 tctgaatatt cacactcttt acaggaactt tttagagcaa taggccatca ggagtatagt
    45181 gatgctcgac agaaacagaa gaagtattct gaatgaaagc gactaattta aggagtgagg
    45241 aaagatggaa agtaatttca ttgactggca tcccgctgac atcattgcag gtttgcgcaa
    45301 gaagggaacg tcaatggcgg cggagtctcg cagaaatggt ttgagttcct caacgctggc
    45361 gaacgcatta tcacgcccat ggccgaaagg agagatgatt attgcgaaag ccctgggaac
    45421 tgacccctgg gttatctggc catcacgcta ccatgatccg cagacccatg agtttatcga
    45481 cagaacgcag ttgatgcgta gctacactaa accgaaaaaa tgagtggtct ggcggtagcc
    45541 ccgcgaacgg ggctgccagc tctcagacaa ttactcgcct ttcacacgct caatatttgc
    45601 acctaaagcg cgtagtttgt cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg
    45661 atcaaccacc gtcgtccctt ccgcaataca gccagccagc accaggcttg ctgatgcacg
    45721 cagatcggtt gccataacct gtgcgccaga aagtttttca acaccgtgac aaataacggt
    45781 attgctttcg atttcggcgt gcgcgcccat acggctcagc tctggcacat gcataaagcg
    45841 gttttcaaag accgtttcgg tgatgaatcc ggtcccttct gccaccaggt tcaacagcgt
    45901 gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc gcggtacgta cgttaacagc
    45961 cttcggacgt ttgccatgca tatccaggct aatccagtct tcgccgactt cgatgtccgc
    46021 tccagcgtca cgcagtttcg ccagcacggc gtccagagta tctggctgcg cgttacggca
    46081 gataattttg ccgcgagaga tcgccgccgc caccaggaaa gtaccggttt cgatacgatc
    46141 cggcagcacg cgatagacac cgccgcctaa acgttccaca ccttcgatga cgatacgatc
    46201 ggtgccctga ccgctaattt tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat
    46261 ttccggttca cgcgctgcgt tttcaataat cgtggtgcct tccgccaggg ttgcagcaca
    46321 catgatggtc accgttgcgc caacgctgac tttatccatc acgatatgtg cgcctttcag
    46381 acgaccatcg acggaagctt taacgtaacc ttcttccagt ttgatggtcg cgcctaattg
    46441 ttcgaggcca gaaatgtgta gatcaaccgg acgtgcgccg atcgtacaac cgccaggcag
    46501 tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc agcgcccaga tagaagcacg
    46561 catggtttta accagatcgt aaggtgcgca gaatacatta acgtcgcggg catcaatatg
    46621 cacagaacca ttacgttcta ctttcgcacc cagctggctt agcagcttca ttgatgtatc
    46681 gacgtctttt agtttcggga cgttctggat ctctaccggt tcttccgcca gtagtgcggc
    46741 aaaaaggata ggcagagcag catttttagc gccggaaatt gtgacttcgc cctggagctt
    46801 cgttggcccc tgaacacgaa atttatccat ttagtttgtt ctcagttaac aattcatatc
    46861 cgctaccggc gaatcgccca tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc
    46921 cgcaggggta tacgctttga tcgacacagc atgaatgcgg ttatccgcaa tatattccat
    46981 cagcgggcca tagaccgtct gctgtttttt aacccgactc atgccgtcaa acaactcacc
    47041 cacggcaata acctgaaagt ggctgccatc gccggaaacg tggacttcct ggagggagag
    47101 agcgttcatc aacacgctct gaatttcatt attttccatg ggatcttcaa tcatcagtta
    47161 ataaatcagc gaaacatctt agagcaaagt tgcgctggca taaataagca aaaagcctcg
    47221 ctgataaatc agacaaggct cgacttgcag gcaggtttgc cggacaggcg gttaacgcca
    47281 tatccggcct gaaaaaattt aacgaggcag aacatcagca ggcaaattat acaatttcgc
    47341 cagggtatac actttgtcgt ttaccccctg aagcgtcaca ttgttgccct gctttttcgc
    47401 cagatcgata agatggagca gcagtgccag tccacccgta tccacgcgag acacacggct
    47461 aagatcgatg ctggtaatcc ccttcaccgc ttcctcacgt atttcccaaa gcggtagcaa
    47521 aacgtcctga tccagctctc cggataacgc cagcgtgtca cccgtctgca tccagctcag
    47581 tgactcgctc attatttttt ctcttccaga gtgattttct gttgagaaat cgatttcagt
    47641 tgcgcagtca ggccgtcgat acctttggta cgcagcagcg ttccccactc gttttgtttg
    47701 gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag cctgccagtt gcccgtctgg
    47761 gagtttttac gccactggaa gtccagacgc accggcgggc ggccattcgg gtcaataatg
    47821 gtaacgcgaa tcggcacaat ggttttatca cccagcggct gttctggcgc aatctgatag
    47881 gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa
    47941 gcggcaaagt aagcatcacg ttgagctggg gtcgcactct tgtaatactg gcccagcacc
    48001 agcgcaccgg cgtatttcac ctgtacgtat ggcaacagtt cctgatcaac aatggtacgc
    48061 agataatccg ggttggcccg aatttgcggt tgctcattct tcaggcgatc gaacgttttc
    48121 tgcgccgcct cgtccatcag cttatacgga ttggtctggt ctgccgcggt tgccgcactc
    48181 agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt taaacatgcg tcggttctcc
    48241 tgaaattatt tcgttgtacc cacaggttca gtggtttcat tattacctgg cgcagcagct
    48301 ggcgcatcgc cactattctt attgtcatcg cctttactac cgtaaaggaa ctgaccaatg
    48361 agatcttcca gcaccatcgc agacttggtg tcctgaattg tatcgccatc cttcaggata
    48421 gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg ccagatattg ttcccccagc
    48481 aggccggaag tacgaatgct cagcgaactg gtatcaggaa tgtggttata acgttgttca
    48541 atttccagcg ttacgcgcgg cagataggtt ttcgggtcca gcgtaatatc cgccacccgg
    48601 cccacaacaa cgccaccaat gctgaccgga gagcgggctt tcaggccgcc aatgttatcg
    48661 aacgtcgcat aaagcgtata ggtcggttca gtacgtatag acgtcacgtt cgctgccttc
    48721 aggcaaacaa acagcgccgc aagcagtgct gctaataaaa agatacccac ccaaatttca
    48781 ttttttttcg tttgcatgaa ctcaattccc aaacatcaat gcggtcagca caaaatccag
    48841 ccccagaaca gccagagacg agtggacaac ggtgcgagtg gttgcccggc taatcccggc
    48901 agacgtcggg atggcgtcgt agccgttaaa caacgaaatc cacgtcaccg tgatggcgaa
    48961 caccacgctc ttaatcagac agttgacgag atccatacgc cagtcgacgg cattttgcat
    49021 tgccgaccag aagaacccac tatcaatgcc tttccaactg acgccgacca gcgatccgcc
    49081 ccagatcccc acggcgacga agataaccgt caacagtggt aatgaaataa ctccagccca
    49141 gaaacgggga gaaataaccc gacgcagcgg atccaccgcc atcatctcca tactggagag
    49201 ttgctctgta gcgcgcatca ggccgatttc tgcggttagc gccgaaccag cacgcccggc
    49261 aaacaacaac gcggcaacca ccggccccag ttcacgcagt agcgataacg ccaccagcat
    49321 acccagactg gtttccgcac tataagtggt cagaaccaga taaccttgca gccccaacac
    49381 cattccgatg aacacgccag aaacaacaat aatcagcatc gacaggacgc cgacattata
    49441 gagctggcgc accagcagcg gtgcatgttt gcgaaattcc ggtttgccga ccagcgcatt
    49501 gaataacatt aacccggccc gcccgaacgt tctcagggtt ttaatccctt tatgtccgag
    49561 cgacgccagc gcatttaaca gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat
    49621 aatcgccggc aggatagcgg aacggaacag gcccgtcagc tatcccgtcc agaaactgac
    49681 gtacgcgcgg atcaggattc gcctgcaacg cctgggcact accatgagcg acaatttttt
    49741 tgtccgccag gatccaggcg tgatccgcaa tacttaacac ttccggcaca tcgtgagaaa
    49801 ccaccacaca agtcacgccc agcgcgctgt tcagctcaga aatcagcttc accagcacgc
    49861 ccatggtaat gggatcttgc ccaacaaagg gctcatcaaa catgatgaga tccggctcca
    49921 gcgcaatcgc acgtgccagc gctgcacgcc gcgccatccc accggaaagt tcagaaggca
    49981 taagtttagc cgctccacgc agccccacgg cctccagctt catcatcacc gtactatgca
    50041 acaatggcgc gggaagttgg gtatgttccc gcagtggata ggcgacgttg tcaaatacgt
    50101 tcatatcagt gaacaacgcc ccggactgaa ataacatgct catccgtttg cgcactgtat
    50161 acaggcgcga acgagacatc gccggaatat tctcaccatc gaaaagaatc tcaccatgat
    50221 ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt tttaccgatg cccgatggcc
    50281 ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga aatattatcg aagatgcagc
    50341 gattgccacg cgtaaaactg acatcgcgca tatcgactaa attcgccaca gactgctcca
    50401 taattcaccc ttcgtcttgc gttgattttc taagcatggc gctcaattta accttgaacc
    50461 caacatattt acagaatatt acctgccgtg gttagcgaaa gctggcattt gttttacttt
    50521 ttagccgcat aaagtcaaaa ttaagaatct gttacggctt tctgaaaatc ttcagcggac
    50581 cggcgagtat acctgaagaa aggacgttag atgcttttag ctacggcact gttaattgtt
    50641 ggtttacttt tggtcgttta cagtgccgac cgcctggttt ttgccgcgtc tattctttgc
    50701 cgaacctttg gcatcccgcc gttgatcatc ggcatgacgg tggtcagtat tggtacatcg
    50761 ttaccagaaa tcatcgtctc gcttgccgcg tctttgcacg aacaacgcga tttagccgtt
    50821 ggtacagccc tcggctcaaa cattatcaat atattgctga tcctcggtct ggctgcgctg
    50881 gttcgtcctt ttaccgtcca ttctgatgtt ctacgccgtg aattaccctt aatgttgttg
    50941 gtcagcgtgg tggccggttc cgtactctat gacggacaac ttagtcgcag cgatggtatc
    51001 tttctcctct ttctggctgt gctatggctg ctgttcattg ttaaacttgc acgtcaggct
    51061 gaacgtcagg ggactgacag cctgaccaga gagcagcttg cagagctgcc gcgtgaaggt
    51121 ggattgcccg tcgcattttt atggctcggc atcgcactga tcattatgcc agtggccacg
    51181 cggatggtag ttgataacgc cacggtgctg gcgaattact ttgccatcag cgagttgacg
    51241 atgggtctga cggcaattgc tatcggaacc agcctgccgg aactggcaac cgcaatagca
    51301 ggggttcgca aaggtgaaaa cgacattgcg gtcggaaata tcattggcgc aaacattttt
    51361 aatattgtca tcgtgttggg tttacccgcg ctgataacgc caggagagat tgatccactg
    51421 gcgtacagtc gtgactacag cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc
    51481 tggcggcgct ccccgcaacc gggccgtggt gtaggggtat tattaactgg cggatttatc
    51541 gtatggctgg cgatgttgta ctggttatcg ccaatactcg ttgaataact ggaaacgcat
    51601 tatgtcgcac gtagagttac aaccgggttt tgactttcag caagcaggta aagaagtcct
    51661 ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac atcaatcaga atttcacgct
    51721 tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc
    51781 ggggcacatc gggcgcaaaa tggcagcaac atttgccagc actggtacac cttcattttt
    51841 cgtccatcct ggtgaagccg cgcatggtga tttaggcatg gtcaccccgc aggatgtggt
    51901 gattgctatc tctaactcag gtgaatccag cgaaatcacg gccttaattc cagtgcttaa
    51961 gcgtcttcac gtaccgttaa tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc
    52021 cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc tgtccgttag ggctggcacc
    52081 gaccagcagc acaaccgcca cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa
    52141 agcacgcggc tttactgctg aagattttgc gctctcacac ccaggcggcg cactgggtcg
    52201 taaacttctg ctgcgcgtaa acgatattat gcatacgggc gatgagatcc cgcatgttaa
    52261 gaaaacggcc agtctgcgtg acgcattgct ggaagttacc cgcaaaaatc ttggtatgac
    52321 tgtcatttgc gatgacaata tgatgattga aggcatcttt accgacggtg atttacgccg
    52381 tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt gccgatgtga tgacgccggg
    52441 gggaatacgc gtgcgcccag gcattctggc cgttgaggca ctgaacttaa tgcagtcccg
    52501 ccatatcacc tccgtgatgg ttgccgatgg cgaccattta ctcggtgtgt tacatatgca
    52561 tgatttactg cgtgcaggcg tagtgtaaag attcaaggat aaacaacaat gagcaaagca
    52621 ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg acgttatggc aaaagcagag
    52681 aacattcgtc tgctgatcct cgacgttgac ggcgtactgt cagatggcct gatttatatg
    52741 ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg acggttatgg cattcgttgt
    52801 gcgctcacct ctgatattga agtcgctatc attaccgggc gaaaggctaa actggtagaa
    52861 gatcgttgtg ccacattggg gatcactcac ttgtatcagg ggcagtcaaa caaactgatc
    52921 gcctttagcg atctgctgga aaaactggcg attgccccgg aaaatgtggc ttatgtcggc
    52981 gatgatctca tcgactggcc ggtaatggaa aaagtgggtt taagcgtcgc cgtggccgat
    53041 gcgcatccac tgttgatccc gcgcgccgat tacgtaacgc gcattgctgg cggtcgtggc
    53101 gcagtgcgag aagtttgcga cttattactc ctggcgcagg gcaaactgga tgaagccaaa
    53161 gggcaatcga tatgagtaaa gccagacgtt gggttatcat tgtgctatca ctggcggttc
    53221 tggtgatgat cggcattaat atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca
    53281 acaatgatcc cacctataaa agcgagcata cggacacgct cgtctataac ccagaagggg
    53341 cactaagcta tcgattgatt gctcaacacg ttgaatatta ttccgatcag gccgtttcgt
    53401 ggtttacgca gccggtactt accacgtttg ataaggataa aatcccgaca tggtccgtaa
    53461 aagcagataa agccaagctg accaatgacc ggatgctcta tttatatgga cacgttgaag
    53521 tcaatgcact cgtgccggac tctcaacttc gcagaatcac gacggataac gcgcagatca
    53581 atctggtgac gcaggatgtt acctctgaag acctcgtcac gttatacgga acaacattta
    53641 actccagcgg tctgaaaatg cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa
    53701 aggttagaac atcctatgaa attcaaaaca aacaaactca gccttaatct tgtgcttgcc
    53761 agctcacttc tggccgccag cattccggca tttgccgtaa ccggagacac tgatcagccg
    53821 atccacattg aatcggacca gcaatctctt gatatgcaag gcaacgtggt tacctttacc
    53881 ggtaatgtta tcgtcaccca gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc
    53941 cgtccgggcg gcgaacaagg taaagaagtg attgacggct acggtaaacc ggcaacgttc
    54001 taccagatgc aggacaacgg taaacccgtt gaaggtcacg cttcccagat gcactacgaa
    54061 ctggcaaaag attttgtcgt tctgacgggt aatgcttatc tgcagcaggt cgatagcaac
    54121 attaagggcg ataagatcac ttacctggtg aaagagcaga aaatgcaggc tttcagcgac
    54181 aaaggcaagc gcgtaaccac cgttctggtg ccgtcgcagt tgcaggacaa aaacaataaa
    54241 ggccagaccc cggcacagaa gaagggtaat taattcgtta tggcaacatt aactgcaaag
    54301 aaccttgcaa aagcctataa aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac
    54361 tccggggaaa ttgtcggtct gctggggcca aacggtgccg gtaagaccac cactttctac
    54421 atggttgttg gcattgtgcc gcgcgatgcg ggcaacatca ttattgatga tgacgatatc
    54481 agtctgctgc ctctgcatgc acgcgcgcgc cgcggtatcg gctatctgcc gcaggaagcc
    54541 tccattttcc gtcgcctcag cgtttacgat aacctgatgg cggtactgca aattcgtgac
    54601 gacttgtctg ctgaacaacg tgaagaccgt gcgaacgagc tgatggaaga gtttcacatt
    54661 gagcacctgc gtgacagcat ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa
    54721 attgcccgcg cactggctgc gaatccgaaa tttattctgc tcgacgaacc gtttgccggg
    54781 gttgacccga tctcggttat cgacattaaa cgcatcattg agcacctgcg cgacagcggc
    54841 ctgggcgtac tgatcactga ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct
    54901 tatatcgtca gtcaggggca tttgatcgcc cacggcacgc ctacagaaat cttacaagac
    54961 gaacacgtta agcgtgtata ccttggggaa gacttcagac tctgataggg tagaagtttg
    55021 cgacgtttta gcaggagagt acgattctga acatgaagca aggtttgcaa ctcaggctta
    55081 gccaacaact ggcgatgacg ccacagctcc aacaggcaat tcgtctgttg cagttgtcga
    55141 cgctggaact tcagcaagag ctacagcagg cgctggagag caatccgctg cttgagcaaa
    55201 tcgacactca tgaagaaatc gacacccgcg aaacgcaaga cagtgaaaca ctggacaccg
    55261 ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc gctcgatgcc agttgggaca
    55321 ccatttacac cgctggtaca ccatccggca ccagcggtga ctacattgat gacgagctgc
    55381 cggtctatca gggcgaaacg acgcagacct tacaggatta cctgatgtgg caggtcgagt
    55441 tgacaccgtt ttccgacact gaccgcgcga ttgctacctc tatcgtcgat gccgttgatg
    55501 acaccggtta tctgactgtc ccgctggaag atattctcga aagtatgggc gatgaagaga
    55561 ttgacatcga cgaagttgaa gccgtcctta agcggatcca acggtttgat ccagtcggtg
    55621 tggcggcaaa agatctgcgt gactgcctgc tgatccaact ctcccaattc gataagacca
    55681 cgccatggct ggaagaggcc agactgatca ttagcgatca tctcgatcta ttagccaatc
    55741 acgacttccg cactttaatg cgcgtcacgc gtctgaaaga agatgtgctg aaagaagccg
    55801 tcaatctgat ccagtcgctc gatccgcgcc ccgggcagtc gatccagact ggcgaacctg
    55861 agtatgtcat tccagatgtg ctggtgcgta agcataacgg ccactggacg gtagaactca
    55921 acagtgacag cattccgcgt ctgcaaatca accagcacta cgcctcgatg tgcaataacg
    55981 cgcgcaatga tggtgatagc cagtttatcc gcagcaatct gcaggatgcc aaatggttga
    56041 tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac
    56101 agcagcaagc cttctttgag caaggtgaag aatatatgaa accgatggta ctggccgata
    56161 tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg cgtgaccacg caaaaatacc
    56221 tgcatagtcc acgaggcatt tttgaactga aatatttctt ttccagtcac gtcaatactg
    56281 aaggcggggg tgaagcctct tccacagcga ttcgtgcgct ggtgaagaaa ttaatcgcgg
    56341 cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac ctctttgctg tcggaacaag
    56401 gtatcatggt ggcacgccgc actgttgcga agtaccgaga gtctttatcc attccgccgt
    56461 caaaccagcg taaacagctc gtttgaccca accgataagg aagacactat gcagctcaac
    56521 attaccggaa ataacgtcga gatcaccgag gcactgcgcg aatttgttac agccaaattt
    56581 gccaaacttg agcaatattt tgaccgaatc aaccaggtct atgttgttct gaaagtggag
    56641 aaagtcaccc acacctcaga tgcaacactg catgtaaacg gtggtgaaat tcatgccagc
    56701 gcggaaggtc aggacatgta cgctgccatt gatggcttaa ttgataagct ggcacgccag
    56761 ttgaccaaac ataaagataa actgaaacaa cactaattgt ccgggcaatt agcatgtgca
    56821 tggcggcctg ttgtgcggca caacgggcca tttgtacggt taatgctccg aacctgttcc
    56881 actgtttgag tgggcaggtt cttaggtgaa attatgacaa ataatgatac aactctacag
    56941 cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg tccactgcca gagcaaaaaa
    57001 cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac ttagcctggc gcctcaggtg
    57061 gtttttgaag ctatcctgac gcgggaaaaa atgggcagta ccggtatcgg caatggtatt
    57121 gccattccgc atggcaaact ggaagaagat actctgcgtg ccgttggcgt tttcgttcag
    57181 ctcgaaacac ctatagcttt cgatgccatc gacaaccaac cggtggactt actttttgcc
    57241 ctgctggtgc cggcagacca aactaaaacg cacctgcata ctctgtcgct ggtggcgaaa
    57301 cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag cccagagcga tgaagagctg
    57361 tatcaaatca ttacggatac cgaaggtact ccggatgaag cgtagttatt cggtaatgtc
    57421 tcttttagac gttgtgagga gaaacagtac atggtactga tgatcgtcag cggacgttca
    57481 ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata tgggttttta ctgcgtggat
    57541 aaccttcccg tagtgttgtt acccgatctg gctcgaactc tggccgatcg agagatttct
    57601 gccgccgtca gcattgatgt tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg
    57661 atgagtaacc tgcctgacgc tttctcaccg caactactgt tcctggatgc cgaccgtaat
    57721 accttaattc gtcgttacag tgacacgcgc cgactgcatc cgctttccag caaaaacctg
    57781 tcgctggaaa gtgctatcga caaagaaagc gatttgctgg agcctctgcg ttcgcgagcg
    57841 gatctgattg tcgatacctc agaaatgtcc gttcacgagc tggcagaaat gctgcgtacc
    57901 cgtctgttgg gtaaacgtga acgtgaactg accatggtct ttgagtcttt cggcttcaaa
    57961 cacggtatcc ctatcgatgc agattacgtc tttgacgtgc gcttcttgcc gaacccacac
    58021 tgggatccga aactgcgtcc aatgacaggt cttgataaac ctgtcgccgc gttcctcgac
    58081 cgccacacag aagtacacaa ttttatctac cagacgcgaa gctatcttga gctatggtta
    58141 cctatgctgg aaaccaacaa ccgtagctac ctgacggtcg ccattggttg taccggcggg
    58201 aagcaccgtt cggtgtatat tgcagagcaa ctggcagact acttccgctc gcgcggtaaa
    58261 aacgtccagt cacgccatcg gacgctggaa aaacgtaaac catgaccgtc aagcaaactg
    58321 ttgaaattac aaacaagctg ggcatgcatg cccggcctgc aatgaagctg tttgaattaa
    58381 tgcagggttt tgacgctgaa gtgctgttac gtaatgacga aggcaccgag gctgaagcca
    58441 acagcgttat tgcgctgctg atgttggatt ctgccaaagg acggcagatt gaagttgaag
    58501 cgaccggtcc acaggaagag gaagcactgg ccgccgttat cgccctcttt aactctggtt
    58561 ttgatgaaga ttaatcttca tcactttgac attcaaatcc cttcaagctc ccccggcaat
    58621 aagaaaagcg cgtactgaaa tcgttatcaa tcaacgtcga ttgtacattt cgtgcgcttt
    58681 ttactttcag aagaacctta agaaaacctt aagaagcatt gtttagattt cgtttaagtt
    58741 aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga ttcgcttatc
    58801 tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc
    58861 ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag agatccgccg
    58921 tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg gaataccgcg
    58981 ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg tgattgccga
    59041 tttcgacggc aagccttcca ttacgctgac cgaatttgca gaacaatgcc gttacgaaga
    59101 agacattgca caactgcgcc agttactgaa acagctgaag cgttatttgc aggataaccg
    59161 tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca gtgagtcaga
    59221 ggttatcccc gtcgtctgcg acaacattgg tgaaagcaca ttgattccac tggcgacctg
    59281 gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta tcgcccaacc
    59341 ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa cgctggccct
    59401 cacttcgcgc gaagcttaat caagctggtg ttgttgcata aacggctcac cgcctaactg
    59461 atacatctgc cgtaaaatcc acgcctgacg gctacgcaca tagcccgatg gcgcggagac
    59521 tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg accgggtaag
    59581 tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa acacaccgtc
    59641 gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc agactgtttc
    59701 tatccccagc gttaatccgg cttccagccc ttttcgaacc cagctacgcc catcccataa
    59761 aaagagattt ttggctgtct gctgagaaat cgttgaagca ccgcgaatac ggttttcatt
    59821 gcgctcgttg tgcgccaggg cttgctcaat ggaagcgaca tcaaagcccc agtgctcagg
    59881 aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat
    59941 actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacttg
    60001 ccgctcgacc attaccgctg agaaaggaac aggcgcaacg ctaaacaacg cgatgccccc
    60061 gccccagaaa atggcgagga caaccattaa ccgcagaaga aaacggcgaa cgaaactaaa
    60121 caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca
    60181 atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgccggggt ggtgacaatc
    60241 ttattgtctt catcaaccac gatatcatcg acaggacacg gcacatgctc cgcgcccatc
    60301 tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgtag cgggaaatcg
    60361 aaaattttcg gcagtatcgc cggtgcaata cacataaaac caagcggttt tccggcctga
    60421 tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagactg
    60481 gcaaaattgc ttaaattctt cgccgcgcca aaaccccccg gcacaatcaa cgcatccagt
    60541 tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca
    60601 atcagcacat tgcgcgtttc cgtcatcgct tcaccagtta aatggttgat aacatcaacc
    60661 tgctgcttat ccggtgcaaa gcaaaccgcc tgcgcaccgc tgcgtgaaat agctaacaac
    60721 gtcaataccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg
    60781 ccaattttct tcattgtgat cttcctttcg caattgactg aaacacatta atttttttaa
    60841 taaaaatgct acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct
    60901 atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt
    60961 tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg
    61021 gggtcatttt ttagtggctt ttgccaccca cgctttcagt acttctacgt cgtgacgcca
    61081 ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcgg gaaggtcagg
    61141 agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc
    61201 tttaatttta tgcccttcct caacaatgcc ttttttatcc tgcgccgtca gattcgactc
    61261 cagcacgctg acatagcccg gcatcattct ctcaaacacc gctaacccgt cggtgatcag
    61321 cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga
    61381 tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt
    61441 cttgatcatc gcggttagcg ccggaacaga aagcggctta ctcagcacat catccattcc
    61501 agcattgagg tactcttgct tgtctttcag cacgttagcg gttaaggcca ccagcggcgg
    61561 taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc
    61621 tggcaactga atatccagca ataccaggtc gtattcgccc ggtttaaaca tctccagcgc
    61681 cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc
    61741 gacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat
    61801 atcgtcttca tcaaacgcat catcaacctc ttctgctacc gacggtgcgt ggatcgtcaa
    61861 cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc ccatattttt
    61921 cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg
    61981 actgtctttc acctggtaat acatggcgaa gattttatcc agctcatcct gcggaatgcc
    62041 gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac
    62101 ggtaacctgg ccttgctgag tgaatttgac ggcgttactg atgaggttcc acaagatctg
    62161 ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg gctccagatt
    62221 aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa
    62281 gctggtgaaa tcaaccggct gattatcaag ctggaccttg cgccgttcca tcttatccat
    62341 gtcgataata tcgttgaaga tattccccag cgtgacagcc gaaacatgga tggttttgag
    62401 atatttttcc tgctcggcgg tgagttcagt atccagcaga atgcggctca ggccgacgat
    62461 accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg ttttgtcgcg
    62521 gctggcgcgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc
    62581 catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca
    62641 ggctttgcgt ccgtcgggat aatccagcca ctgctcatag gtcagtgaca cattatgacg
    62701 gaacactttt tcatcggttt caatgacttt cgcagcggct tctggtgagt aaacatcagc
    62761 aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt
    62821 acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg aagcgtcaag
    62881 gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt
    62941 gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac
    63001 ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc
    63061 gcgctcgcgc atctcctcca gtttttgcac cagtcgtgac agacgttgtc gtgactcctc
    63121 cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa
    63181 gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc
    63241 catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac
    63301 cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat
    63361 tagggaattc cttcacgaca acctgcccca ataataccca attctgacag ttacgttata
    63421 aattgtgtga gaaatgcgga attcccctca atggcctacg ccgttggagg tagccacgga
    63481 cgtcccagcg ccgatccctg tacgccatgt tcattcagat aacgatccag ctcgaccatt
    63541 cccgtccatc gattttcgca ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg
    63601 gaaatacggt ggtagataac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc
    63661 gtgtaatcct ccagttcaat tccgttcaaa cgccccgctt cccaggcttt cgccataatg
    63721 ctgcctttca ctatatgcag cggatgcagc tttatgccgt ccacgccggt ttcaaccact
    63781 cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccaac aattaagtga
    63841 gagcacacct tcagcccacg ctcacgcgcc agctgcgtcg tgcgctgata gcaggcaaaa
    63901 tcatgaccgc gatttatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc
    63961 agccacactt cgtagccctg gtccttatat tcgcaaagca gatccagcac cgcatccggc
    64021 acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc
    64081 tgatacatcg aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag
    64141 gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg
    64201 gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccgcca
    64261 cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc
    64321 accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac
    64381 tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagcccaat
    64441 tcggcgatga cctggatcaa tcgtcccagc ctgcttttat caactgcata accaatcaaa
    64501 attaccgaaa tttcatgcac aatcacataa atcactgttg cttatcttgt gtcagatttt
    64561 tttatcttct tatggatttt aggcaaaaac agtagcatga aacgttatta ccaattaagg
    64621 cagtataaaa tgctggcttt ctcatcaatt caaggcagga taagggttaa cacgccttta
    64681 tgacagtcag gaattggctg tttctctaac gacacccctt ttagccttaa atagaaaatc
    64741 cactttaatt tcatacaatt aataaagaat tttgcgctaa agcacatttc tgtaccaata
    64801 agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc
    64861 tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt
    64921 aaccgatgcg aaaaggacaa caagggggcg aatgcgaggt gcgcgtatga cacgcaaacc
    64981 ccgtcgccac gctcttactg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag
    65041 cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt
    65101 cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca
    65161 cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg
    65221 ttgcggcttg ctgttacaaa aaccggatcg tttttttcgc atcgttgcgc aggagcgcgg
    65281 ctggcgttta gcaaaaaact acgccgtcgg gatgctcttc ctgaataaag atcctgaact
    65341 cgccgctgcc gcacgccgca tcgttgaaga agagctgcaa cgcgaaacgt tgtcgattgt
    65401 gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct
    65461 gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc gcgatatgga
    65521 gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt
    65581 ctacgtctgt agcctgtcga atctggtcaa tatttataaa ggtctgtgta tgccggcgga
    65641 tctgccgcgc ttctacctgg acctcgcgga cctgcgtctg gaatcggcca tctgcctgtt
    65701 tcaccagcgc ttttccacta acaccgtacc gcgctggccg ctggcgcagc cgttccgcta
    65761 cctcgcgcat aacggtgaaa tcaacaccat cactggtaac cgccaatggg cgcgtgcgcg
    65821 tacctataaa ttccagacgc cgctgatccc tgacctgcac gacgccgcgc cgttcgtcaa
    65881 cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactcctgc tggcaggtgg
    65941 gatggatatc atccgtgcca tgcgtctgtt agtgccacct gcctggcaga acaacccgga
    66001 tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga
    66061 tggcccggca ggcatcgtga tgtctgacgg tcgttttgcc gcctgtaacc tcgaccgtaa
    66121 cggtctgcgt ccggcgcgct acgtcatcac caaagataag ctcattacct gcgcctctga
    66181 agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg tggggccagg
    66241 cgaactgatg gttatcgaca cccgcagtgg gcgtattctg cactcggcag aaaccgatga
    66301 cgatctgaaa agccgccacc cttataaaga gtggatggag aaaaacgtcc gccgtctggt
    66361 accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct
    66421 tgccagctac cagaaacaat ttaactacag cgcggaagag ctggattcgg tgatccgcgt
    66481 actgggtgaa aacggtcagg aagcggtcgg ttcgatgggc gacgataccc cattcgccgt
    66541 gctctccagc cagccgcgca ttatttacga ctacttccgc cagcagtttg ctcaggtaac
    66601 caacccgcca atcgacccgc tgcgcgaagc gcatgttatg tcgctcgcca ccagtatcgg
    66661 tcgtgaaatg aacgtcttct gtgaagcaga aggccaggcg caccgtttaa gctttaaatc
    66721 gccgattctg ctctattccg atttcaaaca gctcacgacg atgaaagagg atcactaccg
    66781 cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacggtgaa
    66841 agaactgtgt gacaaagccg aaaaaatggt acgtagcggc accgtgctgc tggtgctctc
    66901 cgaccggaat atcgctaaag atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat
    66961 ccagacccgt ctggtcgagc aaagcctgcg ttgcgatgcc aacatcatcg tagaaaccgc
    67021 cagcgcccgt gatccgcacc acttcgccgt gttgctgggt ttcggcgcga cggctattta
    67081 tccatacctt gcctatgaaa cgctgggccg cctggtagac acccacgcga ttgccaaaga
    67141 ttatcgtacc gtgatgctca actatcgtaa cggcatcaac aaaggcttgt acaaaatcat
    67201 gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt
    67261 cggtctgcac gatgatgtcg tgggcctgtg cttccagggg gcagttagcc gcattggggg
    67321 ggcgagcttt gaagacttcc agcaggatct gctgaatctg tcgaaacgag cctggctggc
    67381 gcgtaagcct atcagccagg gcggcctgct gaaatatgta cacggtggcg aataccacgc
    67441 ctacaacccg gacgtggtgc gcacgctgca acaggcggta cagagcggcg agtatagcga
    67501 ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctac gcgatctgct
    67561 ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact
    67621 gtttaaacgc ttcgataccg ccgcgatgtc tatcggtgcg ttaagcccgg aagcccacga
    67681 ggcgctggcg gaagcgatga acagcatcgg cggtaactca aactccggcg aaggcggtga
    67741 agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtag cgtccggtcg
    67801 ctttggggtt actccggcgt atctggtcaa tgccgacgtc attcagataa aagtcgccca
    67861 gggcgcgaag ccgggtgaag gtggtcagtt accgggtgat aaagtcactc cttacattgc
    67921 caaactgcgc tattcggtgc cgggcgtgac gctgatctcc ccgccgccgc accacgatat
    67981 ctactctatt gaggatttag cgcagctgat tttcgacctc aagcaggtca atccaaaagc
    68041 gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga ctggcgtggc
    68101 aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggcggcaccg gcgcaagtcc
    68161 gctttcatcg gtgaaatacg caggctgtcc gtgggagctg gggcttgttg aaacccaaca
    68221 ggcgctggtt gccaacggct tgcgtcacaa gatccgtttg caggtcgatg gcggcctgaa
    68281 aacgggtgtc gatatcatca aagcggcgat tcttggcgcg gaaagttttg gcttcggcac
    68341 tggcccgatg gtagcgcttg gctgtaaata tctgcgaatt tgccacctga acaactgcgc
    68401 cacgggtgta gcaactcagg atgacaaact gcgtaagaat cactatcacg gcctgccgtt
    68461 caaagtgact aattactttg agtttatcgc ccgtgaaacc cgcgagctga tggcacagct
    68521 gggggtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag agctggacgg
    68581 tttcaccgcc aaacagcaga aactggcact gtcgaagctg ctggagactg ccgagccgca
    68641 tcctggtaaa gcgctctact gcaccgaaaa caacccgccg tttgataacg gcctgctgaa
    68701 cgcgcagttg ctgcaacagg caaaaccgtt tgtcgatgaa cgccagagca aaaccttctg
    68761 gttcgatatc cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca
    68821 gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcactgc
    68881 aggccagagc ttcggcgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggtgatgc
    68941 caacgactat gttggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg
    69001 ttccgccttc cgcagccatg aagcgagcat tatcggcaac acctgcctgt atggcgcgac
    69061 cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc gtaactccgg
    69121 tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga cgggtggtat
    69181 cgtctgtatt ctgggtaaaa ccggcgttaa cttcggcgcg ggcatgactg gcggcttcgc
    69241 ttacgttctc gatgaaagcg gcgatttccg caaacgcgtt aacccggagc tggtcgaggt
    69301 cttaagcgtt gacgatctgg cgatccatga agagcatctg cgtggtctta tcaccgagca
    69361 tgttcagcat accggttccc agcgtggtga agagattctg gcgaactggt caaccttcgc
    69421 cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg
    69481 tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca
    69541 gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat
    69601 ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca
    69661 ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgcc cggtacacaa
    69721 ctacatccca aactggctga agctcgccaa tgaggggcgt atttttgaag cggcggaact
    69781 gtcgcaccag acaaacaccc tgccggaagt ttgcggacgt gtctgcccgc aagaccgtct
    69841 gtgcgaaggt tcctgcaccc tgaacgatga gttcggtgcg gtgaccatcg gcaacattga
    69901 gcgttatatc aatgataaag cgttcgagat gggctggcgt ccggatatgt ccggcgtgaa
    69961 acagaccggt aaaaaagtgg cgattatcgg cgcaggtccg gcgggtctgg cgtgcgcgga
    70021 tgtcctgacg cgcaacggcg taaaagcgat cgtcttcgac cgtcacccag aaattggcgg
    70081 cttgctgacc ttcggtattc cggctttcaa gctggaaaaa gaggtaatga cgcgccgccg
    70141 tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt
    70201 gcagctggac gatctgctga gtgattacga tgccgtgttc cttggcgtcg ggacttatca
    70261 gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt
    70321 ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt
    70381 cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga tggactgcgt
    70441 gcgcacgtct gtacgccagg gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga
    70501 gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggtg ttgagttcaa
    70561 attcaacgtc cagccgctgg gtatagaagt gaatggcaac ggcaaagtca gcggcgtaaa
    70621 aatggtgcgc acagaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagattgt
    70681 ggcaggttcc gaacatatcg ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc
    70741 acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcgcagg ggcgcatcat
    70801 cgccccggaa ggcagcgaca acgctttcca gaccagcaac ccgaaaatct ttgctggcgg
    70861 cgatatcgtc cgtggttccg atctggtcgt taccgccatc gccgaaggtc gcaaagcggc
    70921 agacggcatt atgaactggc tggaagttta agcgaggtaa caatgaattc gccagacagt
    70981 gtctggcgaa tttataaaac gagctatcca actaaatttt aagccattaa atcagcctta
    71041 accttcacta ccaccttttt aatctcacca ggttcgccaa caacgcatcc cggtttatgc
    71101 ggttctcctg gcataaacac ggcgaacatt cccggtttca ggatgatggc ttgctcgttt
    71161 tcaatggcgc tgcaaagctg ataatcatct tcatggtgga actcttcaca ctgacgcgca
    71221 gtgcctgcca tgccaaacag aatccgttcc tcaccgttta ataacagctg gatatcaatg
    71281 tattgttcgt gcaattcggc ttttttctca acgggcgatt gagtattaaa tgtcatgaca
    71341 ttcataaaga tattgtcgcc ctgtaattcg taacggccag gcgctttttc ttgcggtctg
    71401 gcagctaatg ccaacgttaa cgcgtcctgt aacgcaggat gtaatccggc agacggtaat
    71461 gactgtactt cacccatcat cataatattt ctccctgggc caacagcgca gccccaagta
    71521 aacctgcatc gtggcggtag tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct
    71581 gcgccagata cgtttccacc agcgccagat acccttctgc cagaccaacg ctgccaccga
    71641 ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt gcaagcgtac
    71701 gtgcggagcg gtgaatcagt tgctgcgcct gctcatcgcc ctgcccggcg cgcgtgaaaa
    71761 tagttttcgc attcgcacca gccaactccc cctgcgctgc agctgcaatg ccgcgaccag
    71821 aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactggacca tgcggatcgg
    71881 caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtacgcagt ttaccgccgc
    71941 tcactacacc gccgccaacg ccggtggaaa cagtgataaa caccatatcg gttatatcgc
    72001 catccagcgc ctgatactcc gcccatgctg cggcctgcgc atcattaatg gcaatggtcg
    72061 gcaaattggt aagttgctcc agcgttttga ctaacggaaa gtgtaacaat ccaccgagat
    72121 tatgcggatt cagcgccagc aagctgccgt cgcgaataat tccggttgaa gcgatagcaa
    72181 cccgctgcgc atgtgcttgc aacggagaga ctaatgcgga taaggcatca cgcagcgcct
    72241 gcggagtctg gctggcaggc gtaggaagtt ctcggcgatc gcggatctgc ccgtcagcgc
    72301 caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg gtcatagcac
    72361 cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga
    72421 accgaccgtc accgcccacg cgccgtggcg catcgcatcc gccgcctgag caggcgtgtt
    72481 gtaacgccct tcggcaatca cccggcatcc ggcgtcactc aacgttttca ccagcgccag
    72541 atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc
    72601 ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag
    72661 taaaccgtga tggtgaatac gtgccagcag cgtttcaaca ggcaccggac gcggtcggtc
    72721 agtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcaa catcttcaat
    72781 ataggccgtg atgcgtaccg gagaatcctc cagatcgcgc ttcacaatcc caataatcgg
    72841 cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcaatac gaatggcaac
    72901 cgcgcccgcc tgctctgccg ctaatgccat ggcagcgacg atttcgggtt tatcgagcgg
    72961 gctgtccgga accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc
    73021 cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac agagaccggg
    73081 caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa tggcacggca
    73141 ccgctaaatg gtttgccgtc gatagcgtca tgagtacgca atgcttccgg gcgcaaccaa
    73201 cgctgaacac gagaaggcat atccagacca atcagcagga tcaccacaaa cgttaaacta
    73261 aacgagagcg agccaagtgc cgttcccaga tccagacgtt gagcgatcaa cgcgccgagg
    73321 attggagcta gtgcaccgcc cagtgcgcca acgttgtagg taaagcccag ccccgccgca
    73381 cgctggtcgg tatcgaaata accgccgatc agtttcggta aaatccctga gatgccctga
    73441 ccaagcattt gctggaagaa cagtaacaaa ccgagcaccc agacgtttgc gccgccaatc
    73501 gcaaataccg gaataatcag cagctgcgag gccagcagac tacaaacgta cgctttgcgg
    73561 gttcccagcc agtcaccgag gaagccaccg acgcagcacc ccaccgccgc gccaaagcca
    73621 ctaaagaaca gcacattggc gacagtatgc gggtcataag ccagatcggt tttcagatac
    73681 gttggcagca gcgcctgaat cggccatgag tagaggaaag cgaacaacac gaccaccatc
    73741 agcattacgc ccgttggcca gcgtttgccc gtactctgca ccataaagct gataaagatt
    73801 gcggcgcata acagcccgag tacagcgacg attgcggcat tttgcaggtt accggcgaag
    73861 cagaaccaca gcgcagtagc cgccgccagc gtcattacga tattggcaat gcgatgttca
    73921 ccacggtaaa gaatgtctac catcgtgcgt actggtgctt tacccccgtg tttctctttc
    73981 cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg
    74041 ccgataaaga acagcgcacg ccagccccag accggaacca ccagactata gacctgagcg
    74101 gcaacgacgg cccccactga gaagcctgaa atcagaaaac cactggcttt gttacgcaga
    74161 tgctttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc
    74221 atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc
    74281 agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg
    74341 tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc
    74401 agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt gagtaccagg
    74461 gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcc
    74521 cgccattgtg cgcggttaag atggcgatac cacgggatat tctgggttgt agtactcatt
    74581 gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga
    74641 tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaacaactca cccgcgctct
    74701 tgcatcaact gctgagccag cgccttcagt tctggcagat atttttcatc taccggacca
    74761 aacggtttgc ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagc
    74821 ccacggaata cgccagtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt
    74881 ttctgcgcgg tctggatatc gccttctttc agcgccttca cgatcccctg ataacgccag
    74941 cccatgatgt tgtaagtgct gccgataccg ccgtctgcgc ccgccagaag accagaggcg
    75001 aagatttcgt cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgctcc
    75061 atctgataga gatcgccaga ggtctgtttc agcgcaccta cgccaggcag cgtaaccaag
    75121 gtgttgatct gatccagggt cagttttacc ccgctcaggg ctggaatgtt gtacaccacc
    75181 atcggcaacc catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag
    75241 ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc
    75301 gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacatgggc gatgagttta
    75361 atcttacctt tcgcctcttc agcgacgatt tccagtacct gttcgcgctc ggaaaggctt
    75421 tgtacaaagg cttcgccggt cgaaccaccc acgtacaaac cgtcgatgcc ctgctgaata
    75481 ttgaactgaa ccaggcggcg cagactcgct ttatccagtg cttgttgttg gtcaaaagga
    75541 gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg
    75601 cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc
    75661 atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt
    75721 ggtctgaccg aaggcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcgcg
    75781 atcggcttcg tcaggatcat gacggcggat cgcgtcaacg atcgcaatat gctgttgata
    75841 gctaacgttg ttatgctcat gcagagcctg atcggtcacg gttggacgtg cggcgattag
    75901 ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttacccggga tctccgccag
    75961 aacgcggtgg aaatcaacgt ctgagcgaat gaatgccgcg ttgttatcca gcgactgact
    76021 gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc
    76081 atagcgcacc aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc
    76141 aggatgagaa aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga
    76201 aggacgtgag acgcgggcac gttcgccgtt gtttatttgc accagacctt tgcgttttaa
    76261 cgctgccagc gcttcgcgca ccgaaggacg cccgacgtta aagaacgcca tcagttcgcg
    76321 ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc
    76381 ttcttccacc atttcggaga gttttttacg cgccagcggg cggctgcgca agttgcgccc
    76441 gatagtggta gtagaatctt cagcttgcgg atcaaatgcg ttcataggac tcattcagga
    76501 aaatgcgtta gcggataata acgacgctat cgtagcacag tatcaaagtc aggcgaaaag
    76561 atgacagata gcaaaacagg ccacatgggc ctgttttcat tttacttcac aacgcgtaat
    76621 gccggtcgac caccgcgtgg tggctgcgga ggttcatcgt caggatgagt ttcatcatcg
    76681 tgatctggct tgtcgccatc gataaccgac ataacggttt cgttgtctgc cgatgcctct
    76741 tcatcattca tgatgctggc atcttcatcg taggcagctt caggctcaaa catcgtgcct
    76801 gcgccatttt cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc
    76861 ggaatgccac caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg
    76921 acagcacgcg gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc
    76981 tgcacgccag ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac
    77041 cactcataga atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata
    77101 cagattaact ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag
    77161 agtcacgttc aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga
    77221 actcgatgcc cagttgcggc agacgccaca gcagaggagc aaggtagcaa tcgaccaggc
    77281 tgaactcatc gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt
    77341 cttcgcgcag ttgcttacgt gcggcatcgg cttcagaagc tgaaccgttg atgatggtgt
    77401 tcatcagcgt gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag
    77461 ctaccgggta aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa
    77521 tgatgcgaga ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg
    77581 ggttgaggtc aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac
    77641 ttacaccttt ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg
    77701 aaaacagcgt cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata
    77761 gtcagaattt ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccagta
    77821 aggaacgact ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc
    77881 agaaaattgg atgatagttt accagatttt gcgacctttg tggtgagtcg atgccggaaa
    77941 tggggaaaaa gagatgcgct ttagtctgaa atagttgact tagtccctta ttggcgatgt
    78001 ggtttttgtt ttacctgtct gtcaggtggc agcaaaaagc aactttccat tttttacgct
    78061 gatgcagatt ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct
    78121 gccggagcag aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga
    78181 ccgactttct tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt
    78241 tcagaacgca gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca
    78301 ccagcctgac cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc
    78361 atgtcgacca gttccagcgg ctgacgaact accatgcgag cagtttcacg accgaagtac
    78421 tgttccagag aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga
    78481 gctgcggaac ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat
    78541 cccgattaga tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg
    78601 taaactttca gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta
    78661 accgcgattt caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt
    78721 ttgataccac cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg
    78781 gttacagcaa ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga
    78841 gtgtattccg ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc
    78901 agagttttac cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct
    78961 gtaaaagttt tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac
    79021 gtcttcaatt gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct
    79081 atgccagcac acaaaaagtt tcgggaaaaa aaactttctt gtaacgtggg gtcgcaggat
    79141 tatagagaag tcggggtcaa agatcgaccc ctttttgtga tttgtggcag gttttacccc
    79201 gccaaatgct cgcgcttcaa atactcttcg ctttgcatct cttgcagacg tgacaggcaa
    79261 cgctggaact caaacttcag ccgctcgccc tgataaattt catacagcgg cacttctgca
    79321 ctcaccacta atttgacatg gcgctcgtaa aactcatcca ccagcgcaat aaagcgccgc
    79381 gcttcgctct ccatcaaccg cgtcataact ggtacatcaa acaacatgac cgtgtgaaag
    79441 aggcgtgaga gcgcaatata gtcatgctga ctgcgggcgt cgacgcacag cgtagtaaaa
    79501 gaaaccgcca gcgtctggtt ctcgacgccc attgtcgcca atggccgatg gttgatttct
    79561 aacgtcggtg aattttctcg tttcgccccc gccagcgcca accatagttt atccatttgc
    79621 gcccgggttt catcgttgag gggcgaaagc cacagatgcg cctgagtgag tgtacgcagg
    79681 cgataatcaa caccagcgtc cacgttcatt acatcacaat gctgtttaat ggcatcgatt
    79741 gcaggcaaaa aacgcgcacg ttgcaggcca tttcgataaa gttcgtccgg cggaatattt
    79801 gacgtcgcta ccagggtaat accgcgggcg aacagggctt tcatcagacc gccaagtagc
    79861 atggcatcgg taatatcaga aacaaaaaat tcgtcaaaac agagcacgtc agtttctgct
    79921 ttaaagcgat cggcaataat ttccagcgga tcggtctgcc cctgcaaagc agttagctct
    79981 tcatgcaccc gcagcataaa acggtgaaag tgcaggcgct gtttccgctc tcccggcaga
    80041 ctttgataga aaaggtccat cagccaggtt ttcccgcgtc ctacaccgcc ccacatatat
    80101 aagccacgca ctggcatatg ctttgtgtct tcgcgtttac cccacagctt accgacccgc
    80161 gccattagcc cactcgtcct gggggctggt ggcgtgctat tgacgagttc cttataaata
    80221 atttccaggc ggctgacggc ctctttttga acgtcgtcgg gttgatggct gccttcattg
    80281 agcgccttca ggtattgcga tgttggggta acgctttgca tgatcttatt gttattcctt
    80341 gaataatcgg tgcgccgttg ttcacggttg acgaaaaaaa ggccgttcta cactacgcga
    80401 tatgctgtcg ggattccact tctgtggaat taacggttat agtggcataa tcagccgcag
    80461 gcatggagcc tgaagccaac accctacgga aacaaaagac aacgggagat gttcatgacc
    80521 tgggaatatg cgctaattgg gttagtcgtc ggcatcatta ttggtgctgt ggccatgcgt
    80581 tttggtaatc gtaaactacg ccagcaacag gcgttgcagt acgaactgga aaagaataaa
    80641 gctgaactgg acgagtatcg cgaagagctg gttagccact ttgcccgcag cgcggaatta
    80701 ctggatacca tggcgcacga ctatcgccag ctgtatcagc acatggcgaa aagctccagt
    80761 agcctgctgc cggaactgtc tgcggaagca aacccgttcc gtaatcgtct ggcagagtct
    80821 gaagccagca acgatcaggc accggtgcag atgcctcgcg actattctga aggcgcatcc
    80881 ggcctgctgc gtactggcgc gaagcgcgac taatttattt ttggggcgca gccattgcgc
    80941 cctcctcttc tctccctccc cgactatcat ttaatctggt gtctcattgt tagccgtctg
    81001 aaaattcaat aacatcaaac tgttttgaat ctcttttctt atcattcagg tacgagagca
    81061 ggaataatga aaaaacaaac ccagctgttg agtgcattag cgttaagtgt cgggttaact
    81121 ctctcggcgt catttcaggc cgtcgcgtcg attccaggcc aggttgccga tcaggccccc
    81181 ctccccagtc tggctccaat gctggaaaaa gtgcttccgg cagtggtgag cgtacgggtg
    81241 gaaggaacgg ccagtcaggg acagaaaatc ccggaagaat tcaaaaagtt ttttggtgat
    81301 gatttaccgg atcaacctgc acaacccttc gaaggtttag gctccggtgt catcatcaac
    81361 gccaataaag gctatgtgct gaccaacaac catgtgatta atcaggcaca gaaaatcagt
    81421 attcagctca atgatgggcg cgagtttgat gcaaaactga ttggtagcga tgaccagagc
    81481 gatatcgccc tgttgcaaat tcaaaacccg agcaaattaa cgcaaatcgc tattgccgac
    81541 tccgataaat tgcgcgtcgg tgattttgcc gtggcggtcg gtaacccatt tggccttggg
    81601 caaaccgcta cctccgggat tatatctgcg ttaggccgca gcggtctgaa tctcgaaggt
    81661 ctggaaaact ttattcaaac tgatgcctcc attaaccgcg gcaattccgg tggtgcgctg
    81721 ttaaacctta acggtgagtt aattggcatc aacactgcaa tccttgcgcc tggcggcggt
    81781 agcgtcggga ttggatttgc cattcccagt aatatggcgc gaacactggc gcagcagctt
    81841 atcgactttg gagaaatcaa acgcggcctg ttaggtatca aaggcaccga gatgagtgcc
    81901 gatatcgcca aagccttcaa ccttgacgtg cagcgtggcg cgtttgtcag cgaagtgttg
    81961 ccaggttctg gctcggcgaa agcgggcgtc aaagcgggcg atattattac cagcctcaac
    82021 ggcaaaccgc tgaatagctt tgctgagttg cgctctcgta tcgcgaccac cgagccgggc
    82081 acgaaagtga agcttggcct gctgcgtaac ggcaaaccac tggaagtaga agtgacgctc
    82141 gataccagca cctcttcgtc ggccagcgct gaaatgatca cgccagcgct ggaaggtgca
    82201 acgttgagcg atggtcagct aaaagatggt ggcaaaggta ttaagatcga tgaggttgtc
    82261 aaaggaagcc cagctgctca ggctggcttg caaaaagacg atgtgatcat tggcgtcaac
    82321 cgcgatcggg tgaactcgat tgctgaaatg cgtaaagtgc tggcggcaaa accggccata
    82381 atcgccttgc aaatcatacg cggcaatgaa agcctctacc tactaatgcg ttaatgtcgt
    82441 aaaccgggca tcaggctgac gtgtgatgtc cggttaactc gtggtatgct gctgccgttc
    82501 ccttttttaa cgatgcctcc atcatgtttg tgaagctctt acgttccgtt gcgattggat
    82561 taattgtcgg cgctattctg ctggttgcca tgccttcgct gcgcagcctt aacccgcttt
    82621 ccactccgca atttgacagt accgatgaaa cgcctgccag ctataatctg gcggttcgcc
    82681 gcgccgcgcc agcggtggtt aacgtttata accgcggttt gaacaccaac tctcacaacc
    82741 agcttgagat ccgcaccctg ggatctggtg taatcatgga tcaacgcggg tatatcatca
    82801 ccaataaaca cgtgatcaac gacgccgatc agatcatcgt cgccttacag gatggacgtg
    82861 tttttgaagc gttactggtt ggctctgact ctctaaccga tctggcggtg ctgaaaatta
    82921 acgccacagg cggtttacca acgattccga tcaatccacg ccgcgtaccg cacattggcg
    82981 atgtggttct ggcgatcggt aacccgtaca acctcgggca gaccattacc caggggatta
    83041 tcagtgccac gggtcgaatc ggtctgaacc cgaccgggcg gcaaaacttc ctgcaaaccg
    83101 atgcttccat taaccacggt aactctggcg gcgcgctggt gaactcgctg ggcgaactga
    83161 tgggcattaa cacgctgtcg tttgataaga gtaacgatgg cgaaacgccg gaaggtatcg
    83221 gctttgcgat tcctttccag ttagcgacca aaattatgga taaactgatc cgtgatggcc
    83281 gtgtgatccg tggctacatt ggcattggtg gacgtgagat cgccccatta catgcgcaga
    83341 gtggcggcat ggatcaactg caagggatcg tggttaacga agtgtcgcct gatggcccag
    83401 cggcaaatgc aggcatccag gtcaacgatc tgattatctc tgtgaataac aaacccgcta
    83461 tttccgccct ggagacaatg gatcaggtcg cggaaattcg ccctggttcg gtgatcccgg
    83521 ttgtcgtgat gcgtgacgat aaacagttaa cattgcaggt caccattcag gaatatccgg
    83581 cgaccaacta aggtcagcgt aaaaaaacaa aaaccggagt ctgtgctccg gttttttatt
    83641 atccgctaat caattactta ttaacgaact cttcgcccag ggcgatatct ttcttcagcg
    83701 tatccagcat accttccagc gcgttctgtt caaatgcgct cagagtaccg atagatttac
    83761 gctcttccac gccgttttta cccagcagca gcggttgaga gaagaaacga gcgtactgac
    83821 cgtcgccttc aacgtaggca cattcgacaa cgccttgttc gccctgcagt gcgcgaacca
    83881 gagacagacc aaaacgtgca gctgcctggc ccatagacag ggttgcagac ccgccaccgg
    83941 ctttcgcttc aaccacctca gtacccgcgt tctggatacg tttggtcaga tcagccactt
    84001 cctgctcggt aaaactaacg ccaggaacct gtgacagcag cggcagaatg gtaacaccag
    84061 agtgaccacc aataaccggc acttcaactt cgcctggctg tttgcctttc agttccgcaa
    84121 caaaggtgtt ggaacgaatg atatccagcg tggtaacgcc gaacagtttg tttttgtcat
    84181 aaacaccggc ttttttcagc acttcagcag caatcgcaac tgtagtgtta accgggttag
    84241 tgataatacc aatgcacgct ttcgggcagg ttttcgcaac ttgctgtacc aggtttttca
    84301 cgataccggc gttaacgtta aacaggtcgg aacgatccat acccggttta cgcgctacgc
    84361 ctgcagagat aagaacgaca tctgcacctt ccagcgccgg agtcgcatct tcacctgaaa
    84421 aacctttgat tttcacagca gtagggatat ggctcagatc gacagccaca ccgggagtca
    84481 ctggagcgat atcatacaga gagagttctg aacctgaagg cagttgggtt tttaacagta
    84541 gtgcaagcgc ctggccaata ccgccagcag cgccgaggac tgcgactttc atcctaaact
    84601 ccttattata ttgataaact aagatatgtt gctccgctgc cgcgacctta atccacaaaa
    84661 ttgccgttta caatgaccac atctcaagaa tgtgtagtca cgcaagttta gcgtttatgc
    84721 atttaattgc cgtaatcagg aacctaactt acgtaattaa cagtcactta tgcattagcg
    84781 caacattcca acaggtggtg acaatatacc ctaccgctca gccaaaacaa tatcaatttg
    84841 ataacaatta atttactttt aagcagaatt tgcaggccgt gacgcaggca tgtttctcaa
    84901 taacgaaatt tgataaaatc ccgctctttc ataacattat ttcagccttc ttcagggctg
    84961 actgtttgca taaaaattca tctgtatgca caataatgtt gtatcaacca ccatatcggg
    85021 tgacttatgc gaagctcggc taagcaagaa gaactagtta aagcatttaa agcattactt
    85081 aaagaagaga aatttagctc ccagggcgaa atcgtcgccg cgttgcagga gcaaggcttt
    85141 gacaatatta atcagtctaa agtctcgcgg atgttgacca agtttggtgc ggtacgtaca
    85201 cgcaatgcca aaatggaaat ggtttactgc ctgccagctg aactgggtgt accaaccacc
    85261 tccagtccat tgaagaatct ggtactggat atcgactaca acgatgcagt tgtcgtgatt
    85321 cataccagcc ctggtgcggc gcagttaatt gctcgcctgc tggactcact gggcaaagca
    85381 gaaggtattc tgggcaccat cgctggcgat gacaccatct ttactacccc tgctaacggt
    85441 ttcaccgtca aagagctgta cgaagcgatt ttagagctgt tcgaccagga gctttaatct
    85501 ctgccccgtc gcttctggcg gcggggaaaa tgttgctttt gcctatcaac ccactgcttt
    85561 cccctgcgat tcatttaacg aatagtgcgt tttactgcga catatc
//
LOCUS       DAJOTB010000005        79559 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJOTB010000005 DAJOTB010000000
VERSION     DAJOTB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.005"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            109..369
                     /gene="iraP"
                     /locus_tag="OHN28_000335"
     CDS             109..369
                     /gene="iraP"
                     /locus_tag="OHN28_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCQ2295783.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
     gene            470..1885
                     /gene="phoA"
                     /locus_tag="OHN28_000336"
     CDS             470..1885
                     /gene="phoA"
                     /locus_tag="OHN28_000336"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCQ2295784.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            2004..2324
                     /gene="psiF"
                     /locus_tag="OHN28_000337"
     CDS             2004..2324
                     /gene="psiF"
                     /locus_tag="OHN28_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCQ2295785.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2426..3541
                     /gene="adrA"
                     /locus_tag="OHN28_000338"
                     /gene_synonym="dgcC"
     CDS             2426..3541
                     /gene="adrA"
                     /locus_tag="OHN28_000338"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCQ2295786.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3558..4367)
                     /gene="proC"
                     /locus_tag="OHN28_000339"
     CDS             complement(3558..4367)
                     /gene="proC"
                     /locus_tag="OHN28_000339"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCQ2295787.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4487..4945
                     /locus_tag="OHN28_000340"
     CDS             4487..4945
                     /locus_tag="OHN28_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCQ2295788.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5128..5652
                     /gene="aroL"
                     /locus_tag="OHN28_000341"
     CDS             5128..5652
                     /gene="aroL"
                     /locus_tag="OHN28_000341"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCQ2295789.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5702..5893
                     /gene="yaiA"
                     /locus_tag="OHN28_000342"
     CDS             5702..5893
                     /gene="yaiA"
                     /locus_tag="OHN28_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCQ2295790.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6151..6828
                     /locus_tag="OHN28_000343"
     CDS             6151..6828
                     /locus_tag="OHN28_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCQ2295791.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            6900..7184
                     /gene="ppnP"
                     /locus_tag="OHN28_000344"
     CDS             6900..7184
                     /gene="ppnP"
                     /locus_tag="OHN28_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCQ2295792.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7332..7673
                     /locus_tag="OHN28_000345"
                     /pseudo
     CDS             7332..7673
                     /locus_tag="OHN28_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7670..7750
                     /locus_tag="OHN28_000346"
     CDS             7670..7750
                     /locus_tag="OHN28_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2295793.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(7831..8742)
                     /gene="rdgC"
                     /locus_tag="OHN28_000347"
     CDS             complement(7831..8742)
                     /gene="rdgC"
                     /locus_tag="OHN28_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCQ2295794.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8867..9775
                     /gene="mak"
                     /locus_tag="OHN28_000348"
     CDS             8867..9775
                     /gene="mak"
                     /locus_tag="OHN28_000348"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCQ2295795.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            complement(10044..11228)
                     /gene="araJ"
                     /locus_tag="OHN28_000349"
     CDS             complement(10044..11228)
                     /gene="araJ"
                     /locus_tag="OHN28_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCQ2295796.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11354..14497)
                     /gene="sbcC"
                     /locus_tag="OHN28_000350"
     CDS             complement(11354..14497)
                     /gene="sbcC"
                     /locus_tag="OHN28_000350"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCQ2295797.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(14494..15696)
                     /gene="sbcD"
                     /locus_tag="OHN28_000351"
     CDS             complement(14494..15696)
                     /gene="sbcD"
                     /locus_tag="OHN28_000351"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCQ2295798.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            15886..16575
                     /gene="phoB"
                     /locus_tag="OHN28_000352"
     CDS             15886..16575
                     /gene="phoB"
                     /locus_tag="OHN28_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCQ2295799.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16633..17928
                     /gene="phoR"
                     /locus_tag="OHN28_000353"
     CDS             16633..17928
                     /gene="phoR"
                     /locus_tag="OHN28_000353"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCQ2295800.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            18335..19654
                     /gene="brnQ"
                     /locus_tag="OHN28_000354"
     CDS             18335..19654
                     /gene="brnQ"
                     /locus_tag="OHN28_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCQ2295801.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19730..21103
                     /gene="proY"
                     /locus_tag="OHN28_000355"
     CDS             19730..21103
                     /gene="proY"
                     /locus_tag="OHN28_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCQ2295802.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            21259..23076
                     /gene="malZ"
                     /locus_tag="OHN28_000356"
     CDS             21259..23076
                     /gene="malZ"
                     /locus_tag="OHN28_000356"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCQ2295803.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23081..23662)
                     /gene="acpH"
                     /locus_tag="OHN28_000357"
     CDS             complement(23081..23662)
                     /gene="acpH"
                     /locus_tag="OHN28_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCQ2295804.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETHRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            23755..24825
                     /gene="queA"
                     /locus_tag="OHN28_000358"
     CDS             23755..24825
                     /gene="queA"
                     /locus_tag="OHN28_000358"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCQ2295805.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24880..26007
                     /gene="tgt"
                     /locus_tag="OHN28_000359"
     CDS             24880..26007
                     /gene="tgt"
                     /locus_tag="OHN28_000359"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCQ2295806.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            26030..26362
                     /gene="yajC"
                     /locus_tag="OHN28_000360"
     CDS             26030..26362
                     /gene="yajC"
                     /locus_tag="OHN28_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCQ2295807.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26390..28237
                     /gene="secD"
                     /locus_tag="OHN28_000361"
     CDS             26390..28237
                     /gene="secD"
                     /locus_tag="OHN28_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCQ2295808.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28248..29219
                     /gene="secF"
                     /locus_tag="OHN28_000362"
     CDS             28248..29219
                     /gene="secF"
                     /locus_tag="OHN28_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCQ2295809.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29349..29696
                     /locus_tag="OHN28_000363"
     CDS             29349..29696
                     /locus_tag="OHN28_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCQ2295810.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29734..30618)
                     /gene="tsx"
                     /locus_tag="OHN28_000364"
     CDS             complement(29734..30618)
                     /gene="tsx"
                     /locus_tag="OHN28_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCQ2295811.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30917..31456)
                     /locus_tag="OHN28_000365"
     CDS             complement(30917..31456)
                     /locus_tag="OHN28_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2295812.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            31607..32056
                     /gene="nrdR"
                     /locus_tag="OHN28_000366"
     CDS             31607..32056
                     /gene="nrdR"
                     /locus_tag="OHN28_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCQ2295813.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            32060..33163
                     /gene="ribD"
                     /locus_tag="OHN28_000367"
     CDS             32060..33163
                     /gene="ribD"
                     /locus_tag="OHN28_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCQ2295814.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33252..33722
                     /gene="ribE"
                     /locus_tag="OHN28_000368"
     CDS             33252..33722
                     /gene="ribE"
                     /locus_tag="OHN28_000368"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCQ2295815.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33742..34161
                     /gene="nusB"
                     /locus_tag="OHN28_000369"
     CDS             33742..34161
                     /gene="nusB"
                     /locus_tag="OHN28_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCQ2295816.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34239..35216
                     /gene="thiL"
                     /locus_tag="OHN28_000370"
     CDS             34239..35216
                     /gene="thiL"
                     /locus_tag="OHN28_000370"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCQ2295817.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            35194..35712
                     /gene="pgpA"
                     /locus_tag="OHN28_000371"
     CDS             35194..35712
                     /gene="pgpA"
                     /locus_tag="OHN28_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCQ2295818.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(35766..36740)
                     /gene="yajO"
                     /locus_tag="OHN28_000372"
     CDS             complement(35766..36740)
                     /gene="yajO"
                     /locus_tag="OHN28_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCQ2295819.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36795..38657)
                     /gene="dxs"
                     /locus_tag="OHN28_000373"
     CDS             complement(36795..38657)
                     /gene="dxs"
                     /locus_tag="OHN28_000373"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCQ2295820.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(38682..39581)
                     /gene="ispA"
                     /locus_tag="OHN28_000374"
     CDS             complement(38682..39581)
                     /gene="ispA"
                     /locus_tag="OHN28_000374"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCQ2295821.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            complement(39581..39823)
                     /gene="xseB"
                     /locus_tag="OHN28_000375"
     CDS             complement(39581..39823)
                     /gene="xseB"
                     /locus_tag="OHN28_000375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCQ2295822.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            40029..41477
                     /gene="thiI"
                     /locus_tag="OHN28_000376"
     CDS             40029..41477
                     /gene="thiI"
                     /locus_tag="OHN28_000376"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCQ2295823.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41531..42121)
                     /gene="yajL"
                     /locus_tag="OHN28_000377"
     CDS             complement(41531..42121)
                     /gene="yajL"
                     /locus_tag="OHN28_000377"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCQ2295824.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(42084..42995)
                     /gene="panE"
                     /locus_tag="OHN28_000378"
     CDS             complement(42084..42995)
                     /gene="panE"
                     /locus_tag="OHN28_000378"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCQ2295825.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43163..43654
                     /gene="yajQ"
                     /locus_tag="OHN28_000379"
     CDS             43163..43654
                     /gene="yajQ"
                     /locus_tag="OHN28_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCQ2295826.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43782..45146)
                     /locus_tag="OHN28_000380"
     CDS             complement(43782..45146)
                     /locus_tag="OHN28_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2295827.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(45295..46185)
                     /gene="cyoE"
                     /locus_tag="OHN28_000381"
     CDS             complement(45295..46185)
                     /gene="cyoE"
                     /locus_tag="OHN28_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCQ2295828.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(46197..46526)
                     /locus_tag="OHN28_000382"
     CDS             complement(46197..46526)
                     /locus_tag="OHN28_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCQ2295829.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(46526..47140)
                     /locus_tag="OHN28_000383"
     CDS             complement(46526..47140)
                     /locus_tag="OHN28_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCQ2295830.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(47130..49121)
                     /gene="cyoB"
                     /locus_tag="OHN28_000384"
     CDS             complement(47130..49121)
                     /gene="cyoB"
                     /locus_tag="OHN28_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCQ2295831.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(49143..50090)
                     /gene="cyoA"
                     /locus_tag="OHN28_000385"
     CDS             complement(49143..50090)
                     /gene="cyoA"
                     /locus_tag="OHN28_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCQ2295832.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(50550..52025)
                     /gene="ampG"
                     /locus_tag="OHN28_000386"
     CDS             complement(50550..52025)
                     /gene="ampG"
                     /locus_tag="OHN28_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCQ2295833.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(52069..52647)
                     /locus_tag="OHN28_000387"
     CDS             complement(52069..52647)
                     /locus_tag="OHN28_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2295834.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52952..53269
                     /gene="bolA"
                     /locus_tag="OHN28_000388"
     CDS             52952..53269
                     /gene="bolA"
                     /locus_tag="OHN28_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCQ2295835.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            53613..54911
                     /gene="tig"
                     /locus_tag="OHN28_000389"
     CDS             53613..54911
                     /gene="tig"
                     /locus_tag="OHN28_000389"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCQ2295836.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            55157..55780
                     /gene="clpP"
                     /locus_tag="OHN28_000390"
     CDS             55157..55780
                     /gene="clpP"
                     /locus_tag="OHN28_000390"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCQ2295837.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55906..57180
                     /gene="clpX"
                     /locus_tag="OHN28_000391"
     CDS             55906..57180
                     /gene="clpX"
                     /locus_tag="OHN28_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCQ2295838.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            57368..59722
                     /gene="lon"
                     /locus_tag="OHN28_000392"
     CDS             57368..59722
                     /gene="lon"
                     /locus_tag="OHN28_000392"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCQ2295839.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59931..60203
                     /gene="hupB"
                     /locus_tag="OHN28_000393"
     CDS             59931..60203
                     /gene="hupB"
                     /locus_tag="OHN28_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCQ2295840.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            60395..62266
                     /gene="ppiD"
                     /locus_tag="OHN28_000394"
     CDS             60395..62266
                     /gene="ppiD"
                     /locus_tag="OHN28_000394"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2295841.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            62417..62788
                     /locus_tag="OHN28_000395"
     CDS             62417..62788
                     /locus_tag="OHN28_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCQ2295842.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            62918..63316
                     /gene="fadM"
                     /locus_tag="OHN28_000396"
     CDS             62918..63316
                     /gene="fadM"
                     /locus_tag="OHN28_000396"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCQ2295843.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(63368..64063)
                     /gene="queC"
                     /locus_tag="OHN28_000397"
     CDS             complement(63368..64063)
                     /gene="queC"
                     /locus_tag="OHN28_000397"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCQ2295844.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(64128..65828)
                     /locus_tag="OHN28_000398"
     CDS             complement(64128..65828)
                     /locus_tag="OHN28_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCQ2295845.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65928..66746
                     /gene="cof"
                     /locus_tag="OHN28_000399"
     CDS             65928..66746
                     /gene="cof"
                     /locus_tag="OHN28_000399"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCQ2295846.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66899..67357
                     /gene="decR"
                     /locus_tag="OHN28_000400"
     CDS             66899..67357
                     /gene="decR"
                     /locus_tag="OHN28_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCQ2295847.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            67387..69159
                     /locus_tag="OHN28_000401"
     CDS             67387..69159
                     /locus_tag="OHN28_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2295848.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            69152..70933
                     /locus_tag="OHN28_000402"
     CDS             69152..70933
                     /locus_tag="OHN28_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2295849.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     SLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            71114..71452
                     /gene="glnK"
                     /locus_tag="OHN28_000403"
     CDS             71114..71452
                     /gene="glnK"
                     /locus_tag="OHN28_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCQ2295850.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            71482..72768
                     /gene="amtB"
                     /locus_tag="OHN28_000404"
     CDS             71482..72768
                     /gene="amtB"
                     /locus_tag="OHN28_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCQ2295851.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72817..73677)
                     /gene="tesB"
                     /locus_tag="OHN28_000405"
     CDS             complement(72817..73677)
                     /gene="tesB"
                     /locus_tag="OHN28_000405"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCQ2295852.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73895..74467
                     /locus_tag="OHN28_000406"
     CDS             73895..74467
                     /locus_tag="OHN28_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCQ2295853.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(74498..74809)
                     /locus_tag="OHN28_000407"
     CDS             complement(74498..74809)
                     /locus_tag="OHN28_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCQ2295854.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            74972..75068
                     /gene="ffs"
                     /locus_tag="OHN28_000408"
     ncRNA           74972..75068
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OHN28_000408"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            75188..75541
                     /locus_tag="OHN28_000409"
     CDS             75188..75541
                     /locus_tag="OHN28_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HCQ2295855.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DIPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(75583..77133)
                     /gene="pdeB"
                     /locus_tag="OHN28_000410"
     CDS             complement(75583..77133)
                     /gene="pdeB"
                     /locus_tag="OHN28_000410"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCQ2295856.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWYRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(77297..77767)
                     /locus_tag="OHN28_000411"
     CDS             complement(77297..77767)
                     /locus_tag="OHN28_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCQ2295857.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77883..78434)
                     /gene="maa"
                     /locus_tag="OHN28_000412"
     CDS             complement(77883..78434)
                     /gene="maa"
                     /locus_tag="OHN28_000412"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCQ2295858.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(78608..78826)
                     /gene="hha"
                     /locus_tag="OHN28_000413"
     CDS             complement(78608..78826)
                     /gene="hha"
                     /locus_tag="OHN28_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCQ2295859.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78852..79226)
                     /gene="tomB"
                     /locus_tag="OHN28_000414"
     CDS             complement(78852..79226)
                     /gene="tomB"
                     /locus_tag="OHN28_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCQ2295860.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19340 a        19690 c        21290 g        19239 t
ORIGIN      
        1 atatttgcgc aaagtatttc ctttgtcata aaaataatac ttccagacac tatgaagttg
       61 tgaaacataa tgttaacttc tccatacttt ggataaggaa atacagacat gaaaaatctc
      121 attgctgagt tgttatttaa gcttgcccaa aaagaagaag agtcgaaaga actgtgtgcg
      181 caggtagaag ctttggagat tatcgtcacg gcaatgcttc gcaatatggc gcaaaatgat
      241 caacagcggt tgattgatca ggtagagggg gcgctgtacg aggtaaagcc cgatgccagc
      301 attcctgacg acgatacgga gctgctgcgc gattacgtaa agaagttatt gaggcatcct
      361 cgtcagtaaa aagttaatct tttcaacagc tgtcataaag ttgtcacggc cgagacttat
      421 agtcgctttg tttttatttt ttaatgtatt tgtacatgga gaaaataaag tgaaacaaag
      481 cactattgca ctggcactct taccgttact gtttacccct gtgacaaaag cccggacacc
      541 agaaatgcct gttctggaaa accgggcagc tcagggcgat attactgcac ccggcggtgc
      601 tcgccgctta acgggtgatc agaccgctgc tctgcgtgat tctcttagcg ataaacctgc
      661 aaaaaatatt attttgctga ttggcgatgg gatgggggac tcggaaatta ctgccgcacg
      721 caattatgcc gaaggtgcgg gcggcttttt taaaggtatc gatgccttac cgcttaccgg
      781 gcaatacact cactatgcgc tgaataaaaa aaccggcaaa ccggactacg tcaccgactc
      841 ggctgcatca gcaaccgcct ggtcaactgg tgtcaaaacc tataacggcg cgctgggcgt
      901 cgatattcac gaaaaagatc acccaacgat tctggaaatg gcaaaagccg caggtctggc
      961 gaccggtaac gtttctaccg cagagttgca ggatgccacg cccgctgcgc tggtggcgca
     1021 tgtgacctcg cgcaaatgct acggtccaag tgcgaccagt gaaaaatgtc cgggtaacgc
     1081 tctggaaaaa ggcggaaaag gatcgattac cgaacagctg cttaacgccc gtgccgatgt
     1141 tacgcttggc ggcggtgcaa aaacctttgc tgaaacggca accgccggtg aatggcaggg
     1201 aaaaacgctg cgtgaacagg cacaggcgcg tggttatcag ttggtgagcg atgctgcctc
     1261 actgaattcg gtgacggaag cgaatcagca aaaaccccta ttaggactgt ttgctgacgg
     1321 caatatgcca gtgcgctggc aaggaccgaa agcaacgtac cacggtaata tagataagcc
     1381 cgcagtcacc tgtacgccta atccgcaacg taatgacagt gtaccgaccc tggcgcagat
     1441 gaccgacaaa gccattgaat tgttgagtaa aaatgagaaa ggctttttcc tgcaagttga
     1501 aggtgcgtca atcgataaac aggatcacgc tgcgaatcct tgtgggcaaa ttggcgagac
     1561 ggtcgatctc gatgaagccg tacaacgggc gctggaattc gctaaaaagg atggtaacac
     1621 gttggtcata gtcaccgctg atcacgccca tgccagccag atagttgcgc cagataccaa
     1681 agctccgggc ctcacccagg cgctaaatac caaagatggc gcagtgatgg tgatgagtta
     1741 cgggaactcc gaagaggatt cacaagaaca taccggcagt cagttgcgta ttgcggcgta
     1801 tggcccgcat gccgccaatg ttgttggact gaccgaccag accgatctct tctacaccat
     1861 gaaagccgcc ctggggctga aataaaaccg cgcccggcag tgaattttcg ctgccgggtg
     1921 gtttttttgc tgttagcaac cagacttaat gacagatcac gggcgcatac gctcatggtt
     1981 aaaacatgaa gagggatggt gcgatgaaaa ttacattact ggttaccttg cttttcggtc
     2041 tggttttttt aaccaccgtc ggcgctgccg agagaacctt aaccccacaa caacagcgta
     2101 tgacctcttg taatcagcag gcgacggcgc aggcgttgaa aggggatgct cgtaagacct
     2161 acatgagtga ttgcctgaag aacagcaaat ctgcgcctgg tgaaaaaagt ttgacgccac
     2221 agcagcaaaa gatgcgcgaa tgtaataatc aagcaacaca acaatctctg aaaggggatg
     2281 atcgtaataa gtttatgagt gcctgcctca agaaagccgc ctgatacctg atagtgctaa
     2341 cgggtgagct acgaaaatgg ctcacccgaa atttcatact tctgccttta gtcccgtctc
     2401 tataatttgg gaaaattgtt tctgaatgtt cccaaaaata atgaatgatg aaaacttttt
     2461 caaaaaagcg gcggcgcatg gggaggaacc tcctttaact cctcaaaacg aacatcagcg
     2521 gtccgggctg cgcttcgccc gtcgcgtcag actaccccgc gcggttggtc tggctggcat
     2581 gttcttaccg attgcttcaa cgctggtttc gcacccgccg ccgggctggt ggtggctggt
     2641 gttggtcggc tgggcgttcg tctggccgca tttagcctgg cagatagcga gcagggccgt
     2701 cgatccgctt agccgggaaa tttacaactt aaaaaccgat gcagtattag cgggaatgtg
     2761 ggtaggcgta atgggcgtaa acgtgctgcc ttccaccgcg atgttgatga ttatgtgtct
     2821 gaatttgatg ggggcaggcg gcccccgtct gtttgttgcg ggtctggtgt tgatggtggt
     2881 ttcctgcctt gtcaccctcg aactgacggg catcaccgtg tcgttcaata gcgcaccgct
     2941 ggaatggtgg ctctcccttc ccattattgt catttatcct ctgctgtttg gctgggtcag
     3001 ctaccagacg gcgactaaac tggcggaaca taaacgcagg ttgcaggtca tgagtacccg
     3061 cgacggtatg acgggcgtgt ataacagacg tcattgggaa actatgttac gcaatgaatt
     3121 tgataactgt cggcggcata atcgcgatgc aacgttactg attatcgata tcgaccattt
     3181 caagagcatc aacgatacct ggggccatga tgtgggtgat gaagcgattg tggcgcttac
     3241 ccgacagtta caaattaccc tgcgcggtag cgatgtgatt ggtcggtttg gcggcgatga
     3301 gtttgcagta atcatgtccg gtacgccagc tgagagcgcc atcaccgcca tgttacgggt
     3361 gcatgaaggg ctaaacacgt tacgcttgcc gaatacgcca caggtaactt tacggattag
     3421 tgtgggggtt gcgccgctga atccacaaat gagtcactat cgcgagtggt tgaaatcggc
     3481 agatttggcg ctctacaaag caaagaaagc cggacgtaac cgcaccgaag tggcggcctg
     3541 acgtccggca aaagccatca ggatttgctg agtttttctg atttttccat acacttcgtc
     3601 atcgcttcga tcactgcggc acggaagcct ttctcttcca gtacgcgtac cgcctcaatg
     3661 gtggtgcctc ccggtgagca gaccatatct ttcagtgttc ccggatgttc tcccgtttcc
     3721 agcaccattt ttgcggagcc cattaccgcc tgagcggcaa atttatacgc ctgggcgcgt
     3781 ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga taaacataaa gacgtaggct
     3841 ggcgaagaac cgctcacacc gaccaccggg tggatcatcg gctcagcaat cacttccgct
     3901 tcgccaaagc agcggaaaat attcagcaca tcagcggtat cttctggggt taccagcgcg
     3961 tttggcgtta cggaggtcat cccggcatta accagcgcgg gagtgttcgg catggcgcgg
     4021 ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt cgagcgtgat acctgcagca
     4081 atagaaacga ccagagagtc tttattcagg ctggaggtga tttcgctaag cactttaatc
     4141 atgatgcctg gtttaacggc ggcaaaaatg atgtcggcga tttgcgctac ttcttgcgcc
     4201 gattctgcgg cgttgatgcc gaactgttca tgcagggcgg tgactttatc cggggagggg
     4261 gtatataccc agatttgccc tggaagcacc tgaccgctgg caatcagacc gccgagaatg
     4321 gcttttccca tattgccgca gccaataaaa ccgattttct tttccattgc ctcactcctg
     4381 ccgtgaaatt cattgttttg ataatcgctg gcagaagcat aaacagaact atgccggaag
     4441 gcaaaagcgc gacacaatag aggattaccc aacaaaggat gactttatga caatttgggt
     4501 ggatgccgac gcgtgtccca atgtaattaa agagattttg tatcgcgcgg cggaacgtat
     4561 gcagatgccg ctggtactgg tagcaaacca gagtttacgc gtgccgccat cacgatttat
     4621 tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat aacgaaattg ttcggcagtg
     4681 tgaagcgggc gatttggtga tcaccgcaga tatacctttg gctgctgaag ccattgagaa
     4741 aggcgcggcg gcgcttaatc cgcgcggcga acgttatacg ccagcgacca ttcgtgagcg
     4801 cctgacgatg cgcgatttta tggatacctt acgtgccagt gggatccaga ccggaggacc
     4861 agatagtctt tcacaacgtg accgccaggc ttttgctgcg gagctggaga agtggtggct
     4921 ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt tacacttcat tcttgaatat
     4981 ttattggtat agtaagggat gtattgagat tttcacttta agtggaattt tttctttaca
     5041 atcgaaattg tactagtttg atggtatgat cgccattctc atgacaccgg ctttcgccgc
     5101 attgcgacct attggggaaa acccacgatg acacaacctc tttttctgat cgggcctcgg
     5161 ggctgtggta aaacaacggt cggaatggcc cttgccgatt cgcttaaccg tcggtttgtc
     5221 gataccgacc agtggttgca atcacagctc aatatgacgg tcgcggagat cgtcgaaagg
     5281 gaagagtggg cgggattccg cgccagagaa acggcggcac tggaagcggt aactgcgcca
     5341 tccaccgtta tcgccacggg cggcggcatt attctgacgg aatttaatcg tcacttcatg
     5401 caaaataacg ggatcgtggt ttatttgtgt gcgccagtat cagtcctggt taaccgactg
     5461 caagctgcac cggaagaaga tttacggcca accttaacgg gaaaaccact gagcgaagaa
     5521 gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg aagttgcgca tattatcatc
     5581 gacgcaacaa acgaacccag ccaggtgatt tctgaaattc gcagcgctct ggcacagacg
     5641 atcaattgtt gattttcgag cgcctatact taacgttcat cccgtgaaat aaggaagaac
     5701 gatgccaacg aaaccgcctt atcctcgtga agcatatata gtgacgattg aaaaaggaaa
     5761 gccaggacag acggtaacct ggtaccaact cagagccgat catcctaaac cagactcgtt
     5821 gatcagtgaa catccgaccg ctcaggaagc gatggatgcg aaaaaacgct atgaggaccc
     5881 tgacaaagag tgaccgcatc agaccgctcg gaagggattc tgagtgccgc cacaagggat
     5941 ctgcgtcaca tttttcacaa ttcatgtttt tcaaataatt agaatattaa acaataacaa
     6001 tccattactg gaatcatttg gaatctttac attatgcagt gcaggtctgc tgctacgctt
     6061 tttgtcattt gtagcacaag taagtgtcag cagtggtgct taacacttgc ccggtaatta
     6121 acgacgaaat aaaagtaagg tggaagaaca atgagtgcgt cgttggcgat cctgactatc
     6181 ggcattgtac ctatgcagga agttttgccg ctcctgacag aatacattga cgaagataat
     6241 atttcccatc atagcctgct ggggaagtta agccgcgaag aagtgatggc ggagtacgcg
     6301 ccagaagcag gcgaagacac aattctcaca ttattaaatg acaaccatct ggcccatgtt
     6361 tcgcgtcgca aagttgagcg agacctgcaa ggtgtggttg aagtgctcga taatcagggt
     6421 tacgacgtca ttatattaat gagtacagca aacattagta gtatgaccgc gcgtaatacg
     6481 atctttcttg agccgtcgcg gatattgcca ccgctggttt cctctattgt tgaagatcat
     6541 caggtggggg ttatcgtacc ggttgaggag ttgctgaccg ttcaggcgca aaaatggcaa
     6601 attttgcaga aaccgccagt attttcattg ggtaacccca ttcatgattc ggaacaaaaa
     6661 atcattgatg ccgggaaaga attactggca aaaggtgcag atgtcatcat gctggattgt
     6721 ttgggattta accagcgtca tcgcgattta ctgcaaaaac agctcgatgt tcctgtcttg
     6781 ctctctaacg tattgattgc acggctggct gcggaattac tgatgtaatt ttgcgtgaca
     6841 gccagcgcct ctggccccta tagtgaagta gatgttcaac taccagacag ggccagttta
     6901 tgcttcaaag taatgagtac ttttccggca aagtgaaatc gatcggcttt tccagcagca
     6961 gcactggtcg cgccagcgtg ggtgttatgg ttgaaggtga atacaccttc agcaccgctg
     7021 agcctgaaga gatgacggta atcagtggcg cgctgaatgt gttactgcct gacgcgaccg
     7081 actggcaagt gtatgaagcc ggttcggtgt ttaatgttcc cggtcacagc gagtttcatc
     7141 tgcaagttgc cgaacccacc tcttatctgt gccgctatct gtaattcctc gccttcccct
     7201 tgaacgggag ggcatttttc tgaaatgtcc tctctttaac ccataataat atttcctttg
     7261 ctgggatttt ttcgatttcc gatatattca taattcatca aggttgatat caatatcagc
     7321 gaagatctcc agatattgtt gcggaactgg cgacgataaa agataaatca gatgatgaat
     7381 ggtggcgtgc attgctgcaa agtcgcaatg atcatttgcg ccaaacggca ttgcacaacg
     7441 cacatacccc ggtgttgttg ttaacgacat tgactgagcc tcaggagcgg tcgctggcta
     7501 ttaataattc acagctggct gccgatgtga aaacggcgtg gttaaaagag gatccatcat
     7561 tactcttatt tgtcgaacaa cccgatcttt cgctgttacg tgatttagtg aaaaccgggg
     7621 caacgcgaaa aattcgcagt gaagcgcgtc accggcttga ggaaaaacaa tgacacaacg
     7681 cccctggagc aagttacagc ataaaacgca taatattgct gcgctaagaa ttattgcccg
     7741 ctgtagctaa cgattatatt cctggtgtgg cttcgtacgc cggataagac gcggcaggcg
     7801 tcgcatccgg cattaaaggt aaatcagcaa ttaacgttgt gcttcgccac ctaatccttc
     7861 aatcaggttt tgaattaacg ctgccagttc accagtcatc aggatgaaat cggcatcaaa
     7921 gcgctgggcg aaatcttcac ggtcgatatc ttcgttttga tcgcgcagct cgtcgcagaa
     7981 cttcagacgc ttgagcgaac catcgtcgca catcacaaac tgaatgcgct gctgccagtc
     8041 gagcgccagt ttagtcacca cttttccggc ttcaatgtgg ttggtgatct cttcgctggt
     8101 cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc agcaacgatt tcagctcggc
     8161 ttcatcaagc agctggaagc cctgtgccgc gctgccggaa cgtacccatt cggtcagcgt
     8221 cagttcaatc gggttttcca tgctcaatgg tacaaccggt aacgacccca ggcttttacg
     8281 cagcagtgcc agcgtatctt ccgctttttt ggcgctggcg cagtccacca taatcaaacc
     8341 gttaaccgta tcgatccaca tcattgtctg gctaaaacgg ctgaaagcac gcggcagcag
     8401 agagtgcagc acttcgtctt tcagcgaatc tttttcggtt ttcttcagct tacgcacctg
     8461 ttccgcttcc agtttggcga ttttcgcttc cagcgcctgt ttaatcaccg gggacgggag
     8521 gattttttct tctttgcgcg cacagataac aatttgacca ttggcaacgt gcgttaacgc
     8581 atcgctgtgc gatcccatcg gaggtaccca gcccatcttc gccatgtcct ggctgccgca
     8641 tggggtaaat gccatcgagg ctagctgttt ttccatctct tctgcacgca gcgaaatctc
     8701 gcggctaaga cggtaaacca ttaaattttt gaaccacagc atgataattt ccacggcctt
     8761 gtcgttaaat ttagcgggca tgataacgaa ttgtcggcgg ccttgcattg ccaatccggt
     8821 tgtccgtctc tacgctattg atattgaaaa aaataaggag agtaccgtgc gtataggtat
     8881 cgatttaggc ggcaccaaaa ctgaagtgat tgcattgggc gatgcagggg agcagttgta
     8941 ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag actattgaaa cgatcgccac
     9001 gttggttgat atggcggagc aggcaacagg gcagcgcgga acggtaggta tgggcattcc
     9061 aggttcgatt tcgccctaca ccggcgtggt gaagaatgcc aattcaacct ggctcaacgg
     9121 tcagccattc gataaagact taagcgcgcg gttgcagcgg gaagtgcggc tggcaaatga
     9181 cgccaactgt ctggcggttt cagaagcggt agatggcgcg gcagcgggag cgcagacggt
     9241 ttttgccgtg attatcggca cgggatgcgg cgcgggcgtg gcattcaatg ggcgggcgca
     9301 tatcggcggc aatggcacgg caggcgagtg gggacacaat ccgctaccgt ggatggacga
     9361 agacgaactg cgttatcgcg aggaagtccc ttgttattgc ggtaaacaag gttgtattga
     9421 aacctttatt tcaggcacgg gattcgcgac ggattatcgt cgtttgagcg gacatgcgct
     9481 gaaaggcagt gaaattatcc gcctggttga agaaagcgat ccggtagcgg aactggcatt
     9541 gcgtcgctac gagctgcggc tggcaaaatc gctggcacat gtcgtgaata ttctcgatcc
     9601 ggatgtgatt gtcctgggcg gcggaatgag caatgtagac cgtttatatc aaacggttcc
     9661 gcagttgatt aaacaatttg tcttcggcgg agaatgtgaa acgccggtac acaaggcgaa
     9721 acatggtgat tcgagcggcg tacgcggcgc tgcatggtta tggccacaag agtaaaaaac
     9781 ccaggcaatt ggcgtatcat gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa
     9841 gtgagccgtc cggtaggccg gataaggcgc tcgcgccgca tccggcaaca aataacgcgc
     9901 acagtggcgg caaccccccg gtgctccgat gcctgatgcg acactggcgt gtcttatcag
     9961 gcctacaaaa tgaacagtcc cgtaggccgg ataaggcgtt cacgccgcat ccggcaataa
    10021 gcaacgtgca ccgtgactgc aatctacccc agtggtttcg ccagcaccgg actatccgcc
    10081 gcttgctggc gcttatagcg accatacagc agcaacgacg acatcgcagc aaacgaaagc
    10141 agggcggcgg gcagcgccac gtaattatat gccagcccca gcgtcagcat cataccaccg
    10201 caatatgcgc cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat
    10261 aactctccgc ctttggcatt ttgcagtaat aatatttgta gaggtgctga aagggcaaat
    10321 aatcccgcgc aacaaataaa agcaaaaata agcgacgttg ttttcatacc gccgaagaaa
    10381 aagagcatca gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt
    10441 gaataacgtc ctgaaatcct gccacttagc atattcccca gcaccatccc tagcccaact
    10501 aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc
    10561 tttacgtagc tgaaccaggc aaacacacct gcgttgccaa acatcgtagc agcgaaaatt
    10621 aaccacgggg cagggctgcg taaaaagtga aattgttcgc gtagttttcc tttcgcctcg
    10681 tcgcgaatat ccggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaatagcg
    10741 atcaacaaaa aggtgtaacg ccagctaaat tcctgactta aatacgttcc cagtggaatg
    10801 cccagcaaat tggcgactgt catcccggaa accatccctg ccacggcggc ggtgactttg
    10861 ccgggtttga taatttttga taacacgatc gcgccgacgc caaaaaatgc accatgcggg
    10921 aagccggaaa ccagccgacc aatggcgagc atcaggtaag acgaagagag cgtgaacatg
    10981 gcgttgccaa tgacgcacaa cgccaccaga aacaacaaga tatgtttgag tgagtagcga
    11041 ctggaaaaaa gcgcgatgat tggcgcaccg accaccaccc ccagcgcata atacgagatc
    11101 atatgcccgg cggcaggaat cgaaattcct acgttatgcg ccagctccgt gagcacgccc
    11161 ataatgccaa attcggccat ccccaaacca aacgtgccca gagccaaaga taaaataact
    11221 tttttcatac caccatcaca accaccagaa cggctaccaa tcaccgtagc caataaagtg
    11281 atagcttgca ggataacgac cagtcgggcc aagagataat tcgcccctct gtattcatta
    11341 tcctgctgaa tagttatttc actgcaaacg cactttccag tttgctgtag cccagtccat
    11401 tgatcttctt cactttgatc tgcaccggaa tacgctcttt catcgcttca acgtggctaa
    11461 tcaccccgat ggttttgcca ctggcattca gggcatccaa cgcatcaagg gcggtatcca
    11521 gcgtttcgct atccagcgtg ccaaaacctt catcaaggaa cagcgagtca atacgcgttt
    11581 tatggctgac cagatctgaa agcgccagtg ccagcgccag actgacgagg aaactttcgc
    11641 cgccggaaag ggtacgggta tcgcgtaccg catcagcctg ccaggtatca acaacctcaa
    11701 cttccagcgc ctcgctggct ttgcgctgta acagatagcg cccgtgcagc cgggtaagtt
    11761 gctgattagc gagatggact aaattatcca gcgtcagccc ttgggcaaat ttgcggaatt
    11821 tatcgccctc tttggaacct attagcgaat tcagatatcc ccagtcctca acctgctgcg
    11881 tcatttgagc aatttgctgc attaaggttt gttgttgctg acggttatct gcatcctgct
    11941 tcagctgctg gcgaatctcg ccttgactcg tggtgttttc acgcaacttt tggtgagttt
    12001 gcgctaactc ttgctgaatc tgctccaccg tcacagtgag agacaacccg ccaggtcggt
    12061 gttgctgatg ctgtgtcagc gtttctgctg tctgagtgac cagcgtttgc gcctgacggc
    12121 gctggttttc cagattctgc ttgagctgtt ccagctgcgt tagtgtttgt tcatccatta
    12181 gcgccgcaag gaacgcctgc tgatcgtcaa agacgctcgc ctgtagcgcg gtgtcaaact
    12241 gcgcctgggc tttttgcaga ctttgcgccg ccagaacatc ctgttgctgt aacgtctgct
    12301 gctggctgtg taatgcgaga cattgttcat gaacctgccg ccagttttcc agcgccacag
    12361 tttcttcgct gtgcgggagt tcatcacttt gcggcaacgt ttccagaatc ggcgtcagct
    12421 gctgcatacg gttttgcagc gcggttaatt cgttctggcg ttgctgccag ctctgcgctt
    12481 cttgctgacg cgtcgccaac cagctctctt cttcatcttc ctgtggcaat gtcagtgcat
    12541 aacccgccaa tgccgttaag agctgctgct ggcgttgttc aatttgctgt tgatactgga
    12601 taatttgctg attatgcgcg gcaatctgcc cttgtaattc atgccgttgg ctgagtaacc
    12661 gcagctggcg ttcgtgctca tcttgtgcat ccagccacgg ttgaatatcg tcctgtggct
    12721 gcagggtgat attgaggctg gccgtgacgg cttgccattg ttgagtaagt gcttgctcat
    12781 cttgtcggag gctttgcgct tcgttttcat cgcgctgaag ttgctttgtt aaggcgtcca
    12841 gttgcccacg tagcgtcgca ccttcttcgc cgagcttttt cacttctttt tccagcgtta
    12901 atagccgggc ctgattaacg cccggttcca gcgcctgata cgcctcgacc gccgggtggc
    12961 tggtggaacc acaaagtggg caaggctgac ccgcctgtaa ctgtgcgcgc tgggcttcca
    13021 gcgttttaat gcgcgcttcc tgctcgcaaa tggttttcac atcggcaagt tgttgcatct
    13081 tttctttata acgatggcgc attttgttaa gtgcggcatt acgttgcgtc tgctccagag
    13141 tgacgttctg gatagcgacc tgtaactgcg ccagacgttt ttgttgggga acaatctgcc
    13201 catgcagtgc gaccaggcgc tgacgcagtg ggcgttgctc agcatgttgc gcctgggcgc
    13261 tagcaacttc atcggcggtt aacgtcaacg tgatcgccgc aagcgcatta agtttttgct
    13321 cagcatgggt taactgttgc tgccattgcc gcagatgctc gcgatcgctg gtttgttggg
    13381 agaactgcgc acgccaaccc gccagttcgt tgttccactg acggaagcgg tcgtgttcct
    13441 gtaaccaggc attcaggctt tgttgctgct gctgtaattc tgctgactgc ttcgccgcgt
    13501 ggtggcgaat gctcgcacga agcgccattg tgctctgtaa gcgagtattt acttcttcaa
    13561 tctgctggcg agtatgcgcc agcgccgtgc tgtgttctgc gatgcgttcc cagtgcggac
    13621 gaagatttcg tgccggttgt gccagactga gcgccgccag ttgaggttgc gcttgttctt
    13681 cttcggctaa tgcctgttgc aaggcctgtt gacggcggct ggcttcttgc tgcaattcgt
    13741 ccagacgcgt taaccagttt agcgattgtt gttcttgctg ctgcgcggta attaactgtt
    13801 tttcttcgtc agtaagtacc tgcaaactcg ctgtcagcga ttgcacttgt tccggcgtga
    13861 gcaacgcgac gccgctggcc tgcgcttgca gcttctccag ctctgtgcgg gccgatttgt
    13921 gctgctcaaa aaccatcgcc gagatttgcc cgtagatttc agtgccggtt aactcctcga
    13981 gcaattctgc gcgttctttg ggtttggcat tcaggaaggc agcaaattgc ccctgcgaaa
    14041 gcagcatcga acgggtgaag cgcccgtaat ccagtccggt taacgtcgcc gtcagttcca
    14101 gcttatcttt cactttgtcg gcgagaattt tgccgtctgc acagcgcgcc agctctacgc
    14161 gtggcacctg caaattaccg tccggctggt tacgcgcccg attctggctc cagaacgcgc
    14221 gatacgcttc atctttgact tcaaactcca cttccgccag acattcggcg gtatcgcggg
    14281 tcatgagatc attttgcgat tgtgaaacgt tagagaggcg cggagtttcg tgatacagcg
    14341 ccagacaaat ggcgtccagc agggtggttt tccccgcacc ggttgggccg gtaatagcaa
    14401 acagcccgtt gctggcgaac ggctcgcggg tgaaatcaat cttccattcg ccttttaatg
    14461 agttcaggtt tttcaggcgc aggctgagaa ttttcatgct tcgtgttctc cggcgaggct
    14521 atgcaacgtc gtggcgaaaa gatgctgcag acgttgctgc tgtgattcat ccagttcttc
    14581 cagtgccaga cggcgattga acacctcttc gacgctgagt tcgctgaggg tttcacgctg
    14641 ttggctggct aacacacgct cgcgctgttc acgactccgg cgtaccagca atacttcgac
    14701 aggcaatgat tcggttaatg cctggatttt gcgctgaata tcatgcagat actcatcagt
    14761 agtgatttcg atatccagcc agacaggcgg ctcctgtgat acatcgcgcc actgttccag
    14821 ctgtgcggta atcgacgcca gatcgccttt cagcaccgcc atgggttgcg ttaccggtac
    14881 gttcaggttt tccacgctct ctaatttgcc gtttgaaaat gtcaccagat ggacatattt
    14941 actcttaccg cattcatcaa aactcagtgg aatgggggag ccgcaatagc gaacatgttc
    15001 catgccgcca ataagctgcg cgcggtgaat atgcccgagc gcgatgtagt cggctggtgg
    15061 aaagttttgt gccggaaacg cgtccagcgt gccaatataa atgtcacgca cggcgtcact
    15121 tttactggca cccacggtcg ttaaatgtcc agtggcgatg atgggcagag gctgatcgcc
    15181 gcgcagtttg caggcatcgg cataatgttg ttgataataa tcagtaatag ccgccagcaa
    15241 atgctgctgt ttttcaatac cgttaagccc cgcctggctg gtaataatgt cacgcgggcg
    15301 taaaaaaggt atgggacaca gcactgcgcc tggcgtcccg tcgcgacggg gcaatatttg
    15361 cggcgcatgt ccggcgctgg cgaccacggt agtattgagg aacgccatga tatcgcgcga
    15421 ttcattcagc gtggcgaccg agtcatggtt tcctgccagt accaccagat gacagccagt
    15481 ttgctgtaaa ttgacaacaa aacggttgta taacgtgcgg gcgtaactgg gcggcgagcc
    15541 ggtatcgaaa acatcaccgg caacaataat cgcatccacc agatgggctt gtgctgtctc
    15601 cagcagccag tcaagaaaag cctgatgttc agcttcgcgg cttttactgt agaagttctg
    15661 gccgagatgc cagtctgagg tgtgaaggat gcgcataacg gttccctggc gaaaaagcat
    15721 gggcgcgatt atacccaaac agatgtgcca tttgcttttt tctgtgccac ggaaatcaat
    15781 aacctgaaga tatgtgcgac gagcttttca taaatctgtc ataaatctga cgcataatga
    15841 cgtcgcatta atgatcgcaa cctatttatt acaacagggc aaatcatggc gagacgtatt
    15901 ctggtcgtag aagatgaagc tccaattcgc gaaatggtct gcttcgtgct cgaacaaaat
    15961 ggctttcagc cggtcgaagc ggaagattat gacagtgctg tgaatcaact gaatgaaccc
    16021 tggccggatt taattctcct tgactggatg ttacctggcg gctccggtat ccagttcatc
    16081 aaacacctca agcgcgagtc gatgacccgg gatattccag tggtgatgtt gaccgccaga
    16141 ggggaagaag aagatcgcgt gcgcggcctt gaaaccggtg cggatgacta tatcaccaag
    16201 ccgttttctc cgaaggagct ggtggcgcga attaaagcgg taatgcgccg tatatcgccg
    16261 atggcggtgg aagaggtgat tgagatgcag gggctaagtc tcgatccgac atctcaccgg
    16321 gtgatggcgg gcgaagaacc gctggagatg gggccaactg aatttaaact gctgcacttt
    16381 tttatgacgc atcccgagcg cgtgtacagt cgcgagcaac tgttaaatca cgtatgggga
    16441 accaacgtgt atgtggaaga tcgtacggtc gatgtccaca ttcgtcgcct gcgtaaagca
    16501 ctggagcccg gcgggcatga ccgcatggtg cagaccgtgc gcggtacagg gtatcgtttt
    16561 tcaacccgct tttaaggcct tgctcatcgg acgtagagca gggcttatga tttcttaact
    16621 ggagtatctt acgtgctgga acggctgtcg tggaaaaggc tggtgctgga gctgctattt
    16681 tgctgcctcc cggctttcat cctgggtgca tttttgggtt acctgccctg gtttttgctg
    16741 gcatcggtaa caggactgct tatctggcat ttctggaatt tattgcgcct ttcatggtgg
    16801 ctgtgggtgg atcgtagtat gaccccgcca ccggggcgtg gtagctggga accgctacta
    16861 tacggcttac accagatgca gctgcgaaat aaaaaacgcc gccgtgaact gggcaatctg
    16921 attaaacgct ttcgtagcgg cgcggagtcg ctgcccgacg cggtggtgct gaccacggaa
    16981 gagggcggta ttttctggtg caacggtctg gcgcaacaga ttcttggctt gcgctggccg
    17041 gaagataacg ggcagaacat ccttaacttg ctgcgttacc cggagtttac gcaatatctg
    17101 aaaacgcgtg atttctctcg cccgctcaat ctggtgctca ataccgggcg gcatctggag
    17161 attcgcgtca tgccttatac ccacaaacag ttgctgatgg tggcgcgaga tgtcacgcaa
    17221 atgcatcaac tggaaggggc gcggcgtaac ttttttgcca acgtgagcca tgagttacgt
    17281 acgccattga ccgtgttaca gggttacctg gagatgatgg atgagcaacc gctggaaggc
    17341 gcggtacgtg aaaaagcgtt gcacaccatg cgtgagcaga cccagcggat ggaagggttg
    17401 gtgaagcaat tgctgacgct gtcgaaaata gaagccgcac cgacgcattt gctcaatgaa
    17461 aaggttgatg tgccgatgat gctgcgcgtc gttgagcgcg aggctcagac tctgagtcag
    17521 aaaaaacaga catttacctt tgagatagat aacggcctca aggtgtcagg caacgaagat
    17581 cagctacgta gtgcgatttc gaacctggtc tataacgccg tgaatcatac gccggaaggc
    17641 acgcatatca ccgtacgctg gcagcgagtg ccgcacggtg ccgaatttag tgttgaagat
    17701 aacggaccgg gcattgcacc ggagcatatt ccgcgcctga ccgagcgttt ttatcgcgtt
    17761 gataaagcgc gttcccggca aaccggtggt agcggattag ggttagcgat cgtgaaacat
    17821 gcggtgaatc atcacgaaag tcgcctgaat attgagagta cagtaggaaa aggtacacgt
    17881 ttcagtttta ttatcccgga acgtttaatt gccaaaaaca gcgattaatt cgcctttgtc
    17941 atcttttatt gccataagcc agccgatgct ggcttatttt ctttgcagtc gaaatacggg
    18001 cgttagattt tacaatggtt ggtgattttt tgttcgcatg attagccatg tctttttcat
    18061 tgaaatagtg ttttatactg gttggtgatt tcttatcgct atatacctct ggtttttaga
    18121 tcccttcttg ctttaaaacg ttataagcgt ttaaattgcg cttcaggtgc tgtcatactg
    18181 actgcattta cgcggtaaat cgaaaaacta ttcttcgccg cgcctggttg ggagtatttc
    18241 ccgctaaaat tgtttaaata taccgctgta tcatccccag ggattggcac aaaaatttaa
    18301 cgttacaaca ccacatccac aggcagtatg atttatgacc catcaattaa gatcgcgcga
    18361 tatcatcgct ctgggcttta tgacatttgc gttgttcgtc ggcgcaggta acattatttt
    18421 ccctccaatg gtcggcttgc aggcaggcga acacgtctgg actgcggcat tcggcttcct
    18481 cattactgcc gttggcctac cggtattaac ggtagtggcg ctggcaaaag ttggcggcgg
    18541 tgttgacagt ctcagcacgc caattggtaa agtcgctggc gtactgctgg caacagtttg
    18601 ttacctggcg gtggggccgc ttttcgctac gccgcgtaca gctaccgttt cttttgaagt
    18661 gggcattgcg ccgctgacgg gtgattccgc gctgccgctg tttatttaca gcctggtcta
    18721 tttcgctatc gttattctgg tttcgctcta tccgggcaag ctgctggata ccgtgggcaa
    18781 cttccttgcg ccgctgaaaa ttatcgcgct ggtcatcctg tctgttgccg caattatctg
    18841 gccggcgggt tctatcagca cggcgactga ggcttatcaa aacgctgcgt tttctaacgg
    18901 cttcgtcaac ggctatctga ccatggatac gctgggcgca atggtgtttg gtatcgttat
    18961 tgttaacgcg gcgcgttctc gtggcgttac cgaagcgcgt ctgctgaccc gttataccgt
    19021 ctgggctggc ctgatggcgg gtgttggtct gactctgctg tacctggcgc tgttccgtct
    19081 gggttcagac agcgcgtcgc tggtcgatca gtctgcaaac ggtgcggcga tcctgcatgc
    19141 ttacgttcag catacctttg gcggcggcgg tagcttcctg ctggcggcgt taatcttcat
    19201 cgcctgcctg gtcacggcgg ttggcctgac ctgtgcttgt gcagaattct tcgcccagta
    19261 cgtaccgctc tcttatcgta cgctggtgtt tatcctcggc ggcttctcga tggtggtgtc
    19321 taacctcggc ttgagccagc tgattcagat ctctgtaccg gtgctgaccg ccatttatcc
    19381 gccgtgtatc gcactggttg tattaagttt tacacgctca tggtggcata attcgtcccg
    19441 cgtgattgct ccgccgatgt ttatcagcct gctttttggt attctcgacg ggatcaaggc
    19501 atctgcattc agcgatatct taccgtcctg ggcgcagcgt ttaccgctgg ccgaacaagg
    19561 tctggcgtgg ttaatgccaa cagtggtgat ggtggttctg gccattatct gggatcgcgc
    19621 ggcaggtcgt caggtgacct ccagcgctca ctaaatcact gaacatttgt tttaaccacg
    19681 gggctgcgat gccccgtggt tttttattgt gttgatgggt taggaattga tggaaagtaa
    19741 gaacaagcta aagcgtgggc taagtacccg acacatacgc tttatggcac tgggttcagc
    19801 aattggcacc gggctgtttt acggttcggc agatgccatc aaaatggccg gtccaagcgt
    19861 gttgttggcc tatattatcg gtggtatcgc ggcgtatatc attatgcgtg cgctggggga
    19921 aatgtcggta cataacccgg cagccagctc tttctcgcgt tatgcgcagg aaaacctcgg
    19981 cccgctggca ggttacatta ccggctggac ctactgcttt gaaatcctta ttgtcgccat
    20041 cgccgatgtg accgcttttg gtatctatat gggtgtctgg ttcccgacgg tgccgcactg
    20101 gatttgggta ctgagtgtgg tgctgatcat ttgcgccgta aacctgatga gcgtgaaggt
    20161 attcggtgag ctggagttct ggttctcgtt ctttaaagtc gccactatca tcatcatgat
    20221 tgtcgccggt ttcggcatca tcatctgggg gattggcaac ggcgggcaac cgaccggtat
    20281 tcataacctg tggagcaacg gcggcttctt cagtaacggc tggcttggta tggtgatgtc
    20341 gttgcaaatg gtgatgtttg cttacggtgg gatcgaaatt atcgggatta ccgccggtga
    20401 agcgaaagat cctgagaaat cgattccgcg tgcgattaac tccgtgccga tgcgtattct
    20461 ggtgttctac gtcggtacgc tgttcgtcat tatgtctatc tacccgtgga atcaggttgg
    20521 cactgccggt agcccgtttg tgctgacgtt ccagcatatg ggcattacct ttgccgccag
    20581 cattcttaac tttgttgtgc tgaccgcttc gctgtcggca attaacagtg acgtatttgg
    20641 tgtaggccgt atgctccacg gtatggcaga gcagggcagc gcgccaaaaa ttttcagcaa
    20701 aacctcgcgt cgcggtattc cgtgggttac ggtgctggtg atgactaccg cgctgctgtt
    20761 tgcggtgtat ctgaactaca tcatgccgga aaacgtcttc ctggtgatcg cttcgctggc
    20821 aaccttcgcc acggtgtggg tgtggattat gatcctgctg tcgcaaatcg ccttccgtcg
    20881 ccgtttgccg ccagaagaag ttaaggcgct gaaatttaaa gtgccgggtg gggtagcaac
    20941 gaccatcggc ggtttgattt tcctgctctt tattatcggg ttgattggtt atcaccctga
    21001 tacgcgtatc tcgctgtatg ttggtttcgc gtggattgtt gtgctgttga ttggctggat
    21061 gtttaagcgc cgccacgatc gtcagctggc tgaaaaccag taatccttgc gttctgtaag
    21121 ccggttcagg aggaaatcct gatccggcta ttccgaaagt tatccgcccc cgtcctcctc
    21181 ccccaaatat ccttcagatg atgagtgatc ctgcattagg ctatggcaag gtgatcagat
    21241 tttcatcaca ggggaattat gatgttaaat gcatggcatc tgccggtgcc cccatttgtt
    21301 aaacaaagca aagatcaact gctcattaca ctgtggctga cgggcgaaga cccaccgcag
    21361 cgcattatgc tgcgtacaga acacgataac gaagaaatgt cagtaccgat gcataagcag
    21421 cgcagtcagc cgcagcctgg cgtcaccgca tggcgggcgg cgatcgagct ctcctgcgga
    21481 caacctcggc ggcgttatag tttcaaattg ctgtggcacg atcgccagcg ttggtttaca
    21541 ccgcaaggct tcagccgaat gccgccggca cgacaggagc agtttgccat cgatgtaccg
    21601 gatatcggcc cacaatgggc tgcggatcag attttttatc agatcttccc tgatcgtttc
    21661 gcccgtaatc ttcctcgtga agctgaacag gatcatgtct attaccatca cgcagccgga
    21721 caagagatca tattgcgtga ctgggatgaa ccggtcacgg cgcaggctgg cggatcaacg
    21781 ttctatggcg gcgatctgga cgggataagt gaaaaactgc cgtatctgaa aaaacttggc
    21841 gtgacggcgc tgtatctcaa tccggtgttt aaagctccca gcgtgcataa atacgatacc
    21901 gaggattatc gccatgtcga tccgcagttt ggcggtgatg gggcgttgct gcgtttgcga
    21961 cacaatacgc agcagctggg aatacgactg gtgctggacg gcgtgtttaa ccacagtggc
    22021 gattcccatg cctggtttga caggcacaat cgtggcacgg ggggagcttg tcacaacccc
    22081 gaatcaccct ggcgtgactg gtactcgttt agtgatgatg gcacggcgct cgactggctg
    22141 ggctatgcca gcttgccgaa gctggattat cagtcggaaa gtctggtgaa tgaaatttat
    22201 cgcggggaag acagtattgt ccgccactgg ctgaaagcgc cgtggaatat ggatggctgg
    22261 cggctggatg tggtgcatat gctgggggaa gcgggtgggg cgcgcaataa tttgcagcac
    22321 gttgccggga tcaccgaagc ggcgaaagaa acccagccgg atgcgtatat tgtcggcgaa
    22381 cattttggcg atgcacggca atggttacag gccgatgtgg aagatgccgc catgaactat
    22441 cgcggcttca cattcccgtt gtggggattt cttgccaaca ccgatatctc ttacgatccg
    22501 cagcaaattg acgcccaaac ctgtatggcc tggatggata attaccgcgc agggctttct
    22561 catcaacaac aattacgtat gtttaatcag ctcgacagcc acgatactgc gcgatttaaa
    22621 acgctgctcg gtcgggatgt tgcgcgcctg cccctggcgg tggtctggct gttcacctgg
    22681 cctggtgtac cgtgcattta ttacggtgat gaagtgggac tggatggcaa aaacgatccg
    22741 ttttgccgta agccgttccc ctggcaggtg gaaaagcagg attcggcgtt attcgcgctg
    22801 taccagcgaa tgattgcgct gcgtaagaaa agtcaggcgt tgcgtcgtgg cggctgtcag
    22861 gtgctgtatg cggaagataa cgtggtggta tttgtccgcg tgctgaatca gcagcgtgta
    22921 ctggtggcaa tcaaccgtgg cgaagcctgt gaagtggtgc tacccgcgtc gccgttgctt
    22981 aatgtcgcgc aatggcaacg caaagaaggt cacgggcaac tgacggacgg gattctggct
    23041 ttgcctgcca tttcggctac ggtgtggatg aattaacgtc ttataacgcc ttgcgtgacg
    23101 cctgctccat catccgcgga taaaactgcc agaagcgggt ttcgagagca tcgtaatggg
    23161 cgtctaaatc gtaccaggag tcacgcaggg catccagacg tgggcgacgg cttgccatgc
    23221 cgtttaacac gctctggatg aaatccatat cgcgatagcg caccaaccac tgctctgacc
    23281 ataagtagtt gttcagattg ataaagcgtg gcggtgagtc tggaagaata gtcatcactt
    23341 gctcccgggc ataacaggta aattcctgta gcggaaaatc gggcgacagc tgcgaccagt
    23401 ggcgggacag aaagtgatcc cacatcacat ccagcgtaat aggcgcgaca cggtgcgttt
    23461 cattacgaaa ccactcccgc gcttcgcgga cttctggcag attgtcggtt aatacgtcga
    23521 ttcgccgatg catatgaatg ccagccacga cgtcgggcgg aaaactttct tcaggatttc
    23581 cgcgtacgaa atcagccagt aaattgccgg aaagcgagct ttccgcgaga tgggctaaat
    23641 gcaggtgagc taaaaaattc atcgattata ttctatccaa aggggggtaa aggttgcagg
    23701 gagagcgccc cggcactaga ctacccgcct cttattttag tctgagtcag tgtcatgcgc
    23761 gttaccgatt tctcttttga attgcccgaa tccctgattg cccactatcc catgcctgaa
    23821 cgcagtagct gtcgtttact gtcgctggac gggccgacgg gcgcgctgac gcacggtact
    23881 ttcaccgatt tactcgataa gctcaacccc ggcgatcttc tggtttttaa taatacccgc
    23941 gtgatcccgg cgcgtctgtt tgggcgtaaa gccagcggcg gcaagattga agtgctggtt
    24001 gagcggatgc tcgacgacaa acgcattctt gcgcatattc gcgcctcgaa agcgccaaaa
    24061 cctggcgcag aactgctgct gggcgatgac gaaagtatta acgcaacaat gaccgcgcgc
    24121 cacggcgcac tgtttgaagt cgaatttaat gatcaacgtt cggtgctgga tattctcaac
    24181 agcatcggcc atatgccgct gccgccgtac atcgaccgtc cggacgaaga tgcagaccgc
    24241 gaactttatc aaacggttta tagcgaaaaa cccggcgcgg tagcagcacc gaccgccgga
    24301 ctgcactttg acgaaccttt gctggaaaaa ttacgcgcca aaggcgtgga gatggcgttt
    24361 gtgacgttgc acgttggtgc gggcaccttc cagccggtgc gcgtcgacac cattgaagat
    24421 cacatcatgc actcggaata cgctgaagtg ccgcaggatg tggtagacgc ggtactggcg
    24481 gcgaaagcgc gcggtaaccg ggtgattgcg gttggcacca cctcagtacg ttcgctggaa
    24541 agtgcggctc aggcagcgaa aaacgatctc attgaaccgt tctttgacga tacccaaatc
    24601 tttatctatc cgggcttcca gtacaaagtg gtcgatgcgc tggtgacgaa cttccacctt
    24661 ccggaatcga cgctgattat gctggtttcg gcatttgctg gttatcaaca caccatgaat
    24721 gcctataaag cggcggtaga agagaaatat cgctttttta gttacggtga tgcgatgttt
    24781 atcacgtaca atccgcaggc aattaatgag cgcgttgggg agtaattccg cggcgctggt
    24841 ttaaacgttg gactgttttt ctgacgtagt ggagaaaaaa tgaaatttga actggacacc
    24901 accgacggtc gcgcacgccg tggccgcctg gtctttgatc gtggcgtagt ggaaacgcct
    24961 tgttttatgc ctgttggcac ttacggcacc gtaaaaggga tgacgccgga agaagttgaa
    25021 gccactggcg cgcaaattat cctcggcaac accttccacc tgtggctgcg cccgggccag
    25081 gaaatcatga aactgcacgg cgatctgcac gattttatgc agtggaaagg accaatcctt
    25141 accgactccg gcggcttcca ggttttcagc cttggtgata ttcgtaaaat caccgaacag
    25201 ggtgttcact tccgtaaccc gatcaacggc gacccgattt tcctcgaccc ggaaaagtcg
    25261 atggagattc agtacgatct tggttcggat atcgtcatga tctttgatga gtgtacgccg
    25321 tatcctgctg actgggatta tgcaaaacgc tccatggaga tgtctctgcg ttgggcgaag
    25381 cgtagccgtg agcgttttga cagtctcgga aacaaaaatg cgctgtttgg tatcattcag
    25441 ggcagtgttt acgaagattt acgtgatatt tctgttaaag gtctggtaga tatcggtttt
    25501 gatggctacg ctgtcggcgg tctggctgtg ggtgagccga aagcagatat gcaccgcatt
    25561 ctggagcatg tatgcccgca aattccggca gacaaaccgc gttacctgat gggcgttggt
    25621 aaaccagaag acctggttga gggcgtacgt cgcggtatcg atatgtttga ctgcgtaatg
    25681 ccaacccgca acgcccgaaa tggtcatttg ttcgtgaccg atggcgtggt gaaaatccgc
    25741 aatgcgaagt ataagagcga tactggccca ctcgatcctg agtgtgattg ctacacctgt
    25801 cgcaattatt cacgcgctta cttgcatcat ctcgaccgtt gcaacgaaat attaggcgcg
    25861 cgactcaaca ccattcataa ccttcgttac taccagcgtt tgatggcggg tttacgcaag
    25921 gctattgaag agggtaaatt agagagcttc gtaactgatt tttaccagcg tcaggggcga
    25981 gaagtaccac ctttgaacgt tgattaatat taataatgag ggaaatttaa tgagcttttt
    26041 tatttctgat gcggtagcgg caacgggtgc accggcgcaa ggtagcccga tgtctttgat
    26101 tttgatgctg gtggtattcg gtctgatttt ctatttcatg atcctgcgtc cacagcagaa
    26161 gcgcaccaaa gaacacaaaa agctgatgga ctccatcgct aaaggtgatg aagtgctgac
    26221 gaacggtggc ctggtcggtc gcgtaaccaa agtagcggaa aacggctaca ttgctatcgc
    26281 actgaatgac accactgaag tagttattaa acgtgacttc gtagctgccg ttctgccgaa
    26341 aggcaccatg aaggcgctgt aattaaaatt tttccctaag ggaattgccg tgttaaaccg
    26401 ttatcctttg tggaagtacg tcatgctgat cgtggtgatt gtcatcggtc tgctgtatgc
    26461 gcttcccaac ctgtttggtg aggatccggc tgttcagatc actggtgcgc gcggagtcgc
    26521 cgccagtgag caaacgctga tccaggtcca gaaaacgtta caagaagaaa aaataactgc
    26581 taagtctgtg gcactggaag agggcgctat tcttgcgcgc ttcgactcca ctgacaccca
    26641 gctgcgcgct cgtgaagcat taatgggcgt tatgggtgac aaatacgtcg tggcgcttaa
    26701 tcttgccccg gcaacgccgc gctggctggc agctattcat gctgagccga tgaagctcgg
    26761 ccttgacctg cgtggcggcg ttcacttcct gatggaagtg gatatggaca ccgcgcttgg
    26821 caaactccag gaacaaaata tcgatagcct gcgcagcgac ctgcgtgaaa agggcatccc
    26881 gtataccacc gttcgtaaag aaaacaacta cggcctgagc atcactttcc gcgatgctaa
    26941 agctcgtgat gaagccattg cgtatctgag caagcgccat ccggacctgg tgattagcag
    27001 ccagggcagc aaccagctgc gtgcggtaat gagcgatgct cgtctgagtg aagcgcgtga
    27061 atatgcggtg cagcagaaca tcaatatcct gcgtaaccgt gtaaaccaac ttggcgtggc
    27121 ggagccggtg gttcagcgtc agggtgctga ccgtatcgtt gttgaactgc caggtattca
    27181 ggacactgcg cgtgcgaaag agattctggg tgcgacggca acgctggaat tccgtctggt
    27241 aaacaccaac gttgatcagg ccgctgcggc atccggtcgc gtaccgggcg actctgaagt
    27301 gaaacagacc cgcgaaggtc agccggttgt gctgtacaaa cgcgtaattc tgaccggtga
    27361 ccatatcacc gactccactt ccagccagga cgaatacaac cagccgcagg ttaacatctc
    27421 tctcgatagc gcgggtggta acatcatgtc taacttcacc aaggacaaca tcggcaaacc
    27481 gatggcaacc ctgtttgtgg agtacaaaga cagcggtaag aaagatgcaa acggtcgtgc
    27541 ggttctggtg aaacaggaag aggtgattaa tatcgccaac atccagtctc gtctgggtaa
    27601 cagcttccgt attactggca tcaataaccc aagcgaagcg cgtcagcttt cactgctgct
    27661 gcgtgccggt gcgttgatcg cgccgattca gattgttgaa gaacgtacca ttggcccaac
    27721 cctgggtatg cagaacattg agcaggggct ggaggcttgc cttgccggtc tgctggtttc
    27781 tattctgttc atgatcatct tctataagaa gtttggtctg attgcgacca gtgctctgat
    27841 tgccaacttg atcttgattg tcggtattat gtcgctgttg ccaggcgcaa cgctgagtat
    27901 gccaggtatc gcgggtatcg tcttaaccct tgcggtagcg gtcgatgcga acgtactgat
    27961 caacgaacgt attaaagaag agttgagcaa cggacgtact gttcaacagg caattgatga
    28021 aggttatcgt ggcgcattca gttccatctt cgatgcgaac atcaccacgc tgattaaagt
    28081 catcatcctg tacgcagtgg gtaccggggc aattaaaggg ttcgcgatta ctaccggtat
    28141 cggtgtggcg acgtcgatgt ttaccgcgat tgtcggtacg cgtgccatcg taaacctgct
    28201 atatggcggc aagcgcgtca agaagctgtc aatctgagga gtgcgatgtg gcacaggaat
    28261 atactgttga acaactaaac cacggccgta aagtctatga ctttatgcgc tgggactact
    28321 gggctttcgg catctctggt ctgctgttaa tcgccgctat cgttattatg ggcgtgcgcg
    28381 gctttaactg ggggctggat ttcaccggtg gtacggttat tgaaattacg ctcgaaaaac
    28441 cggctgaaat tgacgtaatg cgtgatgcat tgcaaaaagc cggttttgaa gagccgatgc
    28501 tgcaaaactt tggtagcagc cacgacatca tggtccgtat gccgcctgct gaaggcgaaa
    28561 ccggtggtca ggtgctgggc agccaggttc tgaaggtgat taacgaatcc accaatcaga
    28621 atgcggcagt gaagcgtatt gagttcgtcg gtccgagcgt gggggcagac cttgcgcaaa
    28681 ccggtgcgat ggcgttgatg gcggcgctgc tgtctatcct cgtgtacgtt ggtttccgct
    28741 ttgagtggcg actggcggca ggggttgtta ttgcgctggc gcacgacgtt atcattacgc
    28801 tgggtatttt gtcgttattc catatcgaga ttgacctgac cattgtggca tcgttgatgt
    28861 cggttatcgg ttactcgctt aacgacagta tcgtggtatc ggaccgtatt cgtgaaaact
    28921 tccgcaagat ccgtcgcggt acgccttacg aaatctttaa cgtgtccttg acccagacgc
    28981 tgcaccgtac cttgatcaca tccggtacta ccttgatggt tatcctgatg ctgtacctct
    29041 tcggtggtcc ggtactggaa ggcttctcgc tgaccatgct tatcggtgtt tccatcggta
    29101 ctgcatcttc catctatgtg gcatctgcgc tggctctgaa actgggtatg aagcgcgaac
    29161 acatgttgca gcagaaagtg gaaaaagaag gggcggatca gccgtcaatt ctgccttaat
    29221 caagtttcag ctgatgttga aaatcccggt cagaagatcg ggattttttt tgatgtaagg
    29281 tgattgcgaa aatccccgca tcttgggaaa ctgcgcgtaa ccctacattt catccaggta
    29341 actttttcat ggctatcatc ccaaaaaact atgcgcggtt agaaagcggc tatcgcgaaa
    29401 aagcattaaa aatctatccg tgggtctgcg gtcgctgttc ccgcgagttt gtttattcca
    29461 acctgcgtga acttaccgtt caccacattg atcacgacca tactaataac ccggaagatg
    29521 gcagtaactg ggaattactt tgtctctatt gccacgatca tgagcattcg aaatacaccg
    29581 aagcggatca gtatggtacg accgttatcg caggggaaga tgcgcagaaa gatgtcggtg
    29641 aagcgaagta caacccattc gctgatttga aagcgatgat gaacaagaag aagtgataaa
    29701 aaatgccgga tatgaaatcc ggcattctca caattagaag ttgtaaccta ctaccagata
    29761 accaccccag ccggtagagc gaacgttgaa gttgccgttg ccgaagttca gttctgcatc
    29821 gtcattccac tgaccaccgt tatgccagta acgggcaacg acagagtagt gccagtgatc
    29881 gtaattcagc gccagaatat ggctggacgc aatagagtta ttggtacgtg ttttaatacc
    29941 gttgttcgca taaccgctgt catcgcccag gtcggaaccc cagtcgaagt tggtgaagcc
    30001 gatgtagctc agctggccgc cccacagatc ggtaatcggc acaaagtact tcactttgaa
    30061 acggtaaccg tcccactcgt tttcgttcgc tgcgccgtag ttctgccact ggtatttcgc
    30121 atagacgttc atggacaggc tcatcggcag accagtatcg atgtcggtac ccagacccat
    30181 gtaccaggtg ctctggcggc catctttatt acgacccatg tcgtaaatgt agttgttcgc
    30241 gaagtaccac tctttgaacg gaccgaagct aaggtcagta ttggtcagct tgtcaatgga
    30301 gaaacgcggt tcgatttcca taaacagcgg agaaccgtgg ttccagatac cttttgcatc
    30361 ggagttaccg ccgaagaata ccggcgcatc cgcataacca tagaagtcga accagtcttt
    30421 tttagcgaat gcttcgtact caaggtaggt atcgttgcgg agctgcggtc cgaaacgggt
    30481 gtgatagctt ccgacaacgt taacgctctg gtgccaccag tcggaaagat actgcggttt
    30541 gtcgttttca gctgcgttga cagtaaaaga cgaagagagc gccagtaccg caccggctgc
    30601 cagtaatgtt tttttcatat gtatgccact gtttgaaaat cccttgcggg agtgaaaaag
    30661 gcgcaaattg cgtttctaaa tatttcgtat acagaggagc ctattataaa aatcattgct
    30721 cacaaaaata tgtttcgttt cacagttcta tcatttacgt aatcgattgc gttcacgttt
    30781 acacacattc gggcggggat tgtactgact ttcacccttg ttgcaaactt ttactatcaa
    30841 tccaggtgat gtaagcggag taacaaaatg acggagaaag atgttgcggg acgcacaata
    30901 tcgccccgca atacgtttac tgcataacag gttgaatgtc gtggatacgt acaaagccta
    30961 actgatccac agaaataccc tttaactgta acggaatatc aacatcgctg ggggcgagga
    31021 cgctggcagg ggcgttaatc aattgattct gcacattgat ttcctgaaag ttgtctattg
    31081 ttccctgaat ttgcccatat tcaacggttc cgctgaatgc gggtaaaggg tcattggatt
    31141 cgccctgaat acgtagcgtc agggtagtgc catctgtgtc aggcgtaata ttcaccagcg
    31201 acatacgtaa agtaccgatc tggctttcca gtcttgctgg tgtttttgct ccaggcagaa
    31261 ggtaaacccc actgctggat tttgcattca gcttgttttg ctgagtgatt ttgaccgttt
    31321 ctttattgag ttgcgtcatc tcctggttca gcgtgctgac gctgtgtttc atctgtcgca
    31381 cttcactttg ctgcacacag gcgctaaggc tgaacaggct tcccagcagg agcagtcgaa
    31441 aaacgttagt attcataggt cgtccctttc tcgtcgcaat tagcctaatg gtagagggta
    31501 aaagcagtga aagcatcgat cctttatctc aaaagcgttg cgcctttgtt gtatcgtcag
    31561 ttcagggtaa aatagatttc tgttaaccac ctggtcagga cgccgtatgc attgcccatt
    31621 ctgtttcgcc gtggacacta aggtaattga ctctcgtctc gtgggcgagg gttcatccgt
    31681 acgccgccgt cggcagtgtc tggtgtgtaa tgaacgtttc accacctttg aagtggcgga
    31741 gctggttatg ccgcgtgttg taaaaagcaa cgacgtgcgt gaaccgttta atgaagagaa
    31801 attacgtagc ggaatgctgc gggcgctgga aaaacgtccg gtgagttccg atgacgtcga
    31861 aatggcaatc aatcatataa aatcgcagct gcgcgccacc ggtgagcgcg aagtgccgag
    31921 caagatgatt ggcaatctgg tgatggagca attgaaaaag ctcgataaag tcgcctatat
    31981 ccgttttgcc tctgtctacc gcagtttcga agatatcaaa gaatttggcg aagagatcgc
    32041 gcgcctggag gactaagccg tgcaggacga gtattacatg gcgcgggcgc taaagctggc
    32101 gcaacgagga cgttttacca cgcatcccaa cccgaatgtc gggtgcgtca ttgtcaaaga
    32161 tggcgaaatt gtcggtgaag gttaccatca acgagcgggt gaaccgcatg ccgaggtaca
    32221 cgcgttgcgc atggccggcg aaaaagccaa aggcgcaacg gcgtatgtca cactcgaacc
    32281 ctgtagccat catggtcgta cgccaccgtg ttgtgatgca ttaattgctg cgggcgtggc
    32341 gcgcgtggtt gctgcaatgc aagaccccaa tccgcaggtt gctggacgtg gactttaccg
    32401 tctgcaacag gctggcattg acgtcagcca cgggctgatg atgagtgaag ctgagcaatt
    32461 gaataaaggc tttctcaagc ggatgcgcac cggctttcct tatattcagt taaaacttgg
    32521 cgcatcgctt gatggtcgca cggcgatggc gagtggcgaa agccagtgga tcacttcgcc
    32581 tcaggcgcga cgcgacgtac aacgactgcg cgcgcaaagt catgccattt taaccagcag
    32641 cgccacggtg ctggcggatg atccagcctt aacagtgcgt tggtctgaac tggatgaaca
    32701 aactcagggg ctctacccgc aacaaaatct ccgtcagcca gtacgtattg tgattgatag
    32761 ccaaaatcgc gtgacgccgg aacatcgcat tgtgcagcag cccggcgaaa cctggttcgc
    32821 gcgtacgcag gaagattctc gtgagtggcc ggaaacggtg cgtaccttgc tgattccaga
    32881 gcataaaggt cacctggatc tggttgtact aatgatgcaa ctgggtaaac agcagattaa
    32941 cagcatctgg gtggaagcgg ggccaacgct cgctggcgca ttgctacagg cgggtttagt
    33001 cgatgagctg attgtctata tcgcacctaa actattaggc aacgacgccc gtggattatg
    33061 cacgctgcca gggcttgaga aattagccga cgctccccaa tttaaattca aagagatacg
    33121 tcatgtaggc ccggatgttt gcctgcattt agtgggtgca tgatctcccg gctcgaaagg
    33181 gaagcagcgc acgaaatatt atgctaaaat ccgcccccct gcggggccat actcgaaccc
    33241 gaaggaagaa aatgaacatt attgaagcta acgttgctac cccggacgct cgcgtcgcca
    33301 tcaccatcgc gcgtttcaac aactttatca atgacagcct gctggaaggt gcaattgacg
    33361 cactgaaacg tatcggtcag gtaaaagatg aaaacattac cgttgtttgg gtgcctggtg
    33421 cctatgagct gccgctggcg gcgggtgcac tggctaaaac cggtaaatac gacgcggtga
    33481 ttgcgctggg tacggttatt cgtggtggca ctgcccactt tgaatatgtc gctggtggtg
    33541 caagcaacgg cctggcgcat gttgcccagg acagcgaaat tccggttgct tttggtgttc
    33601 tgaccactga aagcattgaa caagcgatcg aacgtgctgg caccaaagct ggcaacaaag
    33661 gtgcagaagc tgcactgacc gcgcttgaaa tgattaatgt attgaaagcc atcaaggcct
    33721 gaaattagta aggggaaatc cgtgaaacct gctgctcgtc gccgcgctcg tgagtgtgcc
    33781 gtccaggcgc tctactcctg gcagttgtcc cagaacgaca tcgctgatgt tgaataccag
    33841 ttcctggctg aacaggatgt aaaagacgtt gacgtcctgt acttccgtga gctgctggcc
    33901 ggggtggcga ctaacaccgc atacctcgac ggactgatga agccgtacct gtcccgtctg
    33961 ctggaagaac tgggccaggt agaaaaagca gtactgcgca ttgcgctgta cgagctgtct
    34021 aaacgtagcg atgtgccata caaagtggcc attaacgaag cgatcgaact ggcgaaatcg
    34081 ttcggcgcag aagacagcca taagttcgtc aacggcgtac tcgataaagc agcacctgtg
    34141 attcgcccta acaaaaagta atatccaagc cggtagattc acggaagacc gttccatgat
    34201 cgccggcctt ttctttttta tctgctgagg cgtaacgtat ggcatgtggc gagttctccc
    34261 tgattgcccg ttattttgac cgtgtaagaa gttctcgtct tgatgtcgaa ctgggcatcg
    34321 gcgacgattg cgctcttctc aatatccccg agaaacagac cctggcgatc agcactgata
    34381 cgctggtggc gggcaaccac ttcctccctg atatcgatcc tgctgatctg gcttataaag
    34441 cactggcggt gaacctaagc gatctggcag cgatgggggc cgatccggcc tggctgacgc
    34501 tggcattaac cttaccggac gtagacgaag cgtggcttga gtccttcagc gacagcttgt
    34561 ttgatcttct caattattac gatatgcaac ttattggcgg cgataccacg cgtgggccat
    34621 tatcaatgac gttgggtatc cacggctttg ttccgatggg acgagcctta acgcgctctg
    34681 gggcgaaacc gggtgactgg atctatgtga ccggtacacc gggcgatagc gctgccgggc
    34741 tggcgatttt gcaaaaccgt ttgcaggttg ccgatgctaa agatgcggac tacttgatca
    34801 aacgtcatct ccgtccgtcg ccacgtattt tacaggggca ggcactgcgc gatctggcaa
    34861 attcagctat cgatctctct gacggtctga tttccgatct cgggcatatc gtgaaagcca
    34921 gcgactgcgg cgcacgtatt gacctggcat tgctgccgtt ttctgatgcg ctttctcgcc
    34981 atgttgaacc ggaacaggcg ctgcgctggg cgctctctgg cggcgaagat tacgagttgt
    35041 gttttacggt gccggaactg aaccgtggcg cgctggatgt ggctctcgga cacctgggcg
    35101 taccgtttac ctgtatcggg caaatgaccg ccgatatcga agggctttgt tttattcgtg
    35161 acggcgaacc tgtcacgtta gactggaaag gatatgacca ttttgccacg ccataaagat
    35221 gtcgcgaaaa gtcgcctgaa aatgagtaat ccgtggcatc tgcttgctgt cggattcgga
    35281 agtggattaa gcccgatcgt tcctggcacg atggggtcgc tggcggcgat tcccttctgg
    35341 tatctgatga cttttttgcc ctggcagctc tattcgctgg tggtgatgct ggggatctgt
    35401 atcggcgtct atctttgtca tcaaacggcg aaagacatgg gcgtgcacga tcacggcagt
    35461 attgtctggg acgaatttat tggtatgtgg attacgctca tggcgctgcc gaccaatgac
    35521 tggcagtggg ttaccgccgg atttgtgatt ttccgtattc tggatatgtg gaagccgtgg
    35581 ccgatccgct ggtttgatcg caatgtacat ggcggcatgg ggatcatgat tgacgatatt
    35641 gtcgccgggg tgatctccgc agtcatcctg tactttattg gacatcactg gccgctgggt
    35701 attctgtcgt agttgtgcac cgatgcctga tgtgacgctt gtcacgtctc atcaggcctg
    35761 tatacctatt taaatcctac gaccggatgc ggtttatacg gcgtttccag ctcggcgatt
    35821 tgttccggct tcaaggtgat atccaccgcg tttagcagtt catcaagctg ttcctcccga
    35881 gaagttccga taatcggtgc ggcaatgccc ggtttactca acaaccaggc cagcgcaact
    35941 tgtgctcgcg ttgcaccaag ttcttcactg acgcccgtta agcgttctgc aatttgtgcg
    36001 tcattttcat cgctttcttg gtagagattt ttccccactt catccgacac cagccgtgcg
    36061 gtggtttctc cccaaggacg cgtcagacgg ccccgcgcca gcgggctcca tggaatcacc
    36121 gccacgcctt cctgataaca cagcggcagc atctcgcgct cttcttcacg ataaatcaga
    36181 ttgtagtgat cctgcatact gacaaactgc gcccagccgt gctgtttttg tagttccagt
    36241 gcttgagcaa actgcgaagc gtgcatggat gacgcgccga tataacgcgc tttcccgact
    36301 tttaccacgt cgttcagggc ttccagcgtc tcttcgatcg gcgtgttgta atcccagcga
    36361 tgaatttgca ggatatcgac ataatccatg ccgagacggc gcaggctgtc gtcgatagag
    36421 cgcaaaattt gtgcacggga taatccttca ggtaaatcac caacgcgatg gaacactttg
    36481 gtcgcaacga ccacgtcttc acgacgggcg aaatcccgca gtgcgcgacc gacgatctct
    36541 tcgctgctgc catcggaata gctattggcg gtatcaaaga aatttatgcc gccttccagc
    36601 gcacgtttaa tgatgggacg gctgctctct tccggcagtg tccatgcgtg attaccgcga
    36661 tctggctcgc caaaggtcat acatccgagg caaagtcggg aaacgcgaag gtcggttttt
    36721 cctaaggggt tgtattgcat gctgccactc ctgccatgaa taatttctta agcatagcag
    36781 gagtggagta gggattatgc cagccaggct ttgattttgg cttccatacc ggtggcatcg
    36841 aggccgagtt cggcgcgcat ttcttcctga gttccttgtg gaataaagaa gtccggcagg
    36901 ccaatgttca gcacgggtac tggtttacga tgggccatca acacttcgtt cacgccgctg
    36961 cctgcaccgc ccataatggc gttttcttct acggtaacca gcgcttcatg gctggcggcc
    37021 atttccagaa ttaacgcttc atcaagcggt ttcacgaaac gcatatcgac cagcgtagcg
    37081 ttcagcgatt cagcgacttt cgccgcttct ggcatcagcg taccaaagtt aaggatcgcc
    37141 agtttctcac cacgacgctt cacaatgcct ttgccaattg gcagtttttc cagtggcgtc
    37201 agttccacgc caactgcgtt gccgcgcggg tagcgcaccg cggacgggcc atcgttatag
    37261 tgatagccgg tatagagcat ctggcgacat tcgttttcat cgctcggggt cataatgacc
    37321 atttccggta tgcagcgcag gtaagagaga tcaaaagcgc cctggtgggt ttgaccgtca
    37381 gcgccaacaa tgcccgcacg gtcgatggcg aacaggaccg gaagcttttg aatcgccacg
    37441 tcatgcagca cctgatcata ggcgcgttgc aggaaggtgg agtagatcgc gacaatgggt
    37501 ttgtagcctc caatcgccag acccgcagca aaggttactg cgtgttgctc ggcaattgcc
    37561 acgtcaaagt agcgatccgg gaatttacgt gaaaactcga ccatgccgga accttcacgc
    37621 atcgccggag taatcgccat cagcttgtta tctttcgctg cggtttcgca caaccagtcg
    37681 ccaaagattt ttgaatagct tggtaaaccg ccgctacttt tcggcagaca accgctggag
    37741 ggatcaaatt taggcacggc gtggaaggtg attgggtctt tttctgccgg ttcataacca
    37801 cgaccttttt tggtcatgat atgcaggaac tgcgggcctt tcaggtcgcg catgttctta
    37861 agcgtggtga taagccccag cacatcgtga ccgtccaccg ggccgatgta gttaaagccc
    37921 agctcttcaa acaacgtgcc aggcactacc atgcctttaa tatgttcttc ggtacgtttg
    37981 agcagctctt taatgggtgg cacgccagag aaaacttttt ttccgccttc gcgcagtgaa
    38041 gagtaaagct taccggaaag cagctgcgcc agatggttgt tgagcgcgcc aacattttcg
    38101 gaaatcgaca tttcattgtc gttgagaacc accagcatat caggacggat atcgcccgcg
    38161 tgattcatcg cttcaaacgc catgccagca gtaatcgcgc catcgccaat gacacagacg
    38221 gtgcggcgat ttttgccttc tttctcggca gcaaccgcaa taccaattcc ggcactgatg
    38281 gaggttgatg aatgcccgac gcttaatacg tcatattcgc tttcgccgcg ccacgggaac
    38341 gggtgcaggc cacctttctg acggatggtg ccgattttgt cgcggcgccc ggtcaaaatt
    38401 ttatgcggat aagcctgatg ccccacatcc cagattaact ggtcaaacgg ggtattgtag
    38461 acatagtgca gcgccacggt cagttcgacc gtgcccagcc cggaggcgaa gtgcccgctg
    38521 gaacggctca cgctgtcgag taaatagcgg cgcagttcgt cgcagagttt cggtaaactc
    38581 tctttcggca acagtcgtaa ctcctgggtg gagtcgacca gtgccagggt cgggtatttg
    38641 gcaatatcaa aactcatcag gggcctatta atacttattg tttatttatt acgctggatg
    38701 atgtagtccg ctagcgcttc cagtgccgag gtatcgaatg actgttcagt cagttgtttc
    38761 agcgactgac gggcatcgtc gatcagatcc cgggctttct tccgggcttg ctcaagaccc
    38821 agaagtgcag ggtaggtact tttaccaagt tgctggtcgg caccctggcg ttttcccaac
    38881 gttgcagtat ctcccaccac atccaggatg tcatcctgaa cctggaaggc aaggccgatg
    38941 ctctctgcgt acttgtcgag tactggcaga gcacgacgtc ctttatctcc ggcgcttaat
    39001 gcaccaaggc gaacggcggc gcgaatcaat gcgccggttt tatgacgatg aatacgctca
    39061 agcgcgtcca gaggtacgtg tttgccttca gcgtctaaat ctaatgcctg accaccgcac
    39121 attccggcaa taccgctggc gctcgccaac tcagaaatca tcgaaattct gtcgcgatct
    39181 gacacttccg gcatatcggc atcgcttaga atcgagaacg ccagcgtttg taaagcgtca
    39241 ccagcgagaa tcgcgtttgc ttcgccaaac ttcacatggc aggtcggcaa accgcgacgc
    39301 agatcgtcat catccatcgc cggtaaatca tcatgaatta atgagtaagc gtggatacac
    39361 tctacggcgg cagcgggtgc gtccagtgtg tttgtgctaa cgccgaacat atgaccagtg
    39421 gcataaacca ggaaaggtcg caggcgctta ccacctaata atgcgccata ctgcatggtt
    39481 tcgaccacgg gagtgttctg aaagggcagt ggggcgataa aacggctcag cgcctggttg
    39541 gcctgcttaa cgcaggcttc gagttgctgc ggaaagtcca ttactcattg tccggtgtaa
    39601 aaggggttag agaggcgtct tcattgtcag acagcagaat ttgtacgcgc tgttcggctt
    39661 gttgtaattt agcctgcccc tgacgtgcca gctgcacgcc gcgttcgaac tcgttcagcg
    39721 cctcttccag cggcaggtcg ccactttcca gacgggttac aatctgttcc agctcgctca
    39781 gcgccttttc aaagctggcg ggagcctcat ttttcttcgg cataatgaat gtctgactct
    39841 caatattttt cgccccgtca tggtaacgga ctcagggcaa atagcaaata acgcgcaatg
    39901 gtaaggtgat gtgcgcagca aagcgatgtt agtggtatac ttccgcgcct ggatgcagcc
    39961 gcaggtgtgg gctgctgtat ttttccctat acaagtcgct taaggcttgc caacgaacca
    40021 ttgccgccat gaagtttatc attaaattgt tcccggaaat caccatcaaa agccaatctg
    40081 tgcgcttgcg ctttataaaa atccttaccg ggaacattcg taacgtttta aagcactatg
    40141 atgagacgct cgctgtcgtc cgccactggg ataacatcga agttcgcgca aaagatgaaa
    40201 accagcgtct ggctattcgc gacgccctga cccgtattcc gggtatccac catattctcg
    40261 aagtcgaaga cgtgccgttt accgacatgc acgatatttt cgagaaagcg ttggttcaat
    40321 atcgcgatca gctggaaggc aaaaccttct gcgtacgcgt gaagcgccgc ggcaaacatg
    40381 attttagctc gattgatgtg gaacgttacg tcggcggcgg tttaaatcag catattgaat
    40441 ccgcgcgcgt gaagctgacc aatccggaag tgactgtcca tctggaagtg gaagacgatc
    40501 gtctgctgct gattaaaggc cgctacgaag gtattggcgg tttcccgatc ggcacccagg
    40561 aagatgtgtt gtcgctcatt tccggtggtt tcgactccgg tgtttccagt tatatgttga
    40621 tgcgtcgcgg ctgccgcgtg cattactgct tctttaatct cggcggcgcg gcgcatgaaa
    40681 ttggtgttcg tcaggtggcg cattatctgt ggaaccgttt tggtagctcc caccgcgtgc
    40741 gttttgtcgc cattaacttt gagccggtcg tcggtgaaat tctcgagaaa atcgacgacg
    40801 gtcagatggg cgttatcctc aaacgtatga tggtgcgtgc cgcgtccaaa gtggcagaac
    40861 gttacggcgt acaggcgctg gttaccggtg aagcgctcgg ccaggtgtcc agccagacgc
    40921 tgaccaacct gcgcctgatt gataacgtct ccgatacgct gatcctgcgt ccgctgatct
    40981 cttacgacaa agagcacatc atcaacctgg ctcgccagat tggcaccgaa gactttgccc
    41041 gcacgatgcc ggaatattgc ggcgtgattt ccaaaagccc gacggtgaaa gcggttaaat
    41101 cgaagattga agcggaagaa gagaagttcg acttcagcat tctcgataaa gtggtggagg
    41161 aagcgaataa cgttgatatc cgcgaaatcg cccagcagac cgagcaggaa gtggtggaag
    41221 tggaaaccgt caatggcttc ggcccgaacg acgtgatcct cgatatccgt tctatcgatg
    41281 aacaggaaga taagccactg aaagtcgaag ggattgatgt ggtttctctg ccgttctata
    41341 aactgagcac caaatttggc gatctcgacc agagcaaaaa ctggctgttg tggtgtgagc
    41401 gcggggtgat gagccgcctg caggcgctct atctgcgcga gcagggcttt aacaatgtga
    41461 aggtgtatcg cccgtaatat gtggttgtaa cgtcgcatct ggtcagatgc gacgcttacc
    41521 gcatccgaca ctactcgtaa taattataaa tccctgccgc catcaccagt tgtgatgcca
    41581 cttcatgggc tttctcgcgc ccaaccagca ggtcgataat tttcagacca aagtcgatag
    41641 ctgtacccgg cccctggctg gtcagcaatt ttacccgtgc atcccagacg acgcgcttgt
    41701 cctgccattg ttcggcggga attttgtctt tcagcgttgg gaagccggtc atattgccaa
    41761 tcgggaagat atcgtgcggg accagcacgg tggctggcgc ggcgcaaata gccgcgacga
    41821 tacgcccgga acggtggaac tgtttaacgg tttcaaccag cagggtgcta tcgcgaaaac
    41881 actctgcgcc tttgatgcca ccaggcagca caatcacgtc atattcgcca tcagccactt
    41941 cgaccagcgg cgcatccgcc agcagcttca cgccgcgcga gcaggtaatc gccaggttac
    42001 catcgctggc gacgctggca gtggtgactt tgataccgcc gcgaaccaag aggtcgatag
    42061 tggtgacggc ttcagtctct tcactaccag gggcgaggca aaccagtgcc gatgcgctca
    42121 tattcactct cctttctttt taccatttca aacaggcggg tgttttccgg tacggcaatc
    42181 ccatgcgcgc gggcgcggcg taagagaaaa ccattgatat agtcgatttc ggtgtggcgc
    42241 agcgcgcgga tatcctgcaa catcgacgag atattttccg ctgttgcatc aatcacctgc
    42301 atcacgtaat cacgcaaatc ttctgctgaa gtatgatgcc cttcgcgttc gatcactgcc
    42361 gcgacttctt cgcatatctg cataatttct tgcggatgat gacgtaattc accattcggg
    42421 caattccaga tggcggtcag tggattaatc acacagttga ctgccagctt gcgccacagc
    42481 tcggcgcgaa tattgttatg ccacgcgacg tcaggcaata cggtttgcaa aatatccgcc
    42541 agataactgt aatcgccgtc ctgttgccgt gccggaccaa tatgcgtgat accgtttgcc
    42601 acatgaataa tgacattgcc gtcgcggcgg gcggcatggg tggtggtgcc catcagtaat
    42661 ggctgctgaa tgttttgcaa ctcttcgatg gtgcccatgc cgttgtgaat taacagtatt
    42721 ggcgtggtta caggcagtgt ggacgcgagg cttttgacgg catcggaaac ctgccatgct
    42781 ttcagcgtca ccaggagcag atcgctggtg gcgagaaaat cgggatcgtt ggcggtcagc
    42841 gattcattaa atatcgaacc atctgtctca accagattca cactacaata aggttgcggt
    42901 acgcgcagcc agccctgaac gtcgtgaccc tgtttacaaa gtgctgtaag ccataattgc
    42961 cctaaggcac cgcatcccaa tacggtaatt ttcattgttc ctcctcaccc gcaaccactc
    43021 cgggtgttca ataaggctat cccttaattg tgcatgctgt tgcgactatg cacaattaag
    43081 ggatacgtcc tggtgcagga ctgttggtta tttaactttg caggtattat gcttcgcatc
    43141 aaaaatgaag ggagaggaaa agatgccatc tttcgatatt gtctctgaag ttgatcttca
    43201 ggaagcacgt aacgcggtcg ataacgcgag ccgcgaagtg gagtcccgtt ttgacttccg
    43261 taacgttgaa gcctcatttg agctgaatga cgccagcaaa accatcaaag tgttgagcga
    43321 gtccgacttc caggtcaatc agttgctgga tattctgcgt gccaagttgc tgaagcgcgg
    43381 cattgaaggc agttcactgg atgtaccgga aaatatcgtt catagcggta aaacctggtt
    43441 tgtggaagcg aaactgaaac agggcattga gagcgcgact cagaagaaaa tcgtcaaaat
    43501 gatcaaagac agcaaactga aagtgcaggc gcaaattcag ggcgatgaga tccgtgtgac
    43561 gggcaaatct cgtgatgatt tacaggctgt catggcgatg gtacgtggtg gcgatctggg
    43621 tcagccgttc cagttcaaaa acttccgcga ttaatcgcga caacctgcgc tttgttcatg
    43681 tcagatgcgg catgaacgcc tgattcggcc tacaaaggtt tgtaactcag gaacttagta
    43741 ggcctgataa gcgtagcgca gcaggcaatt tcgcctcgct tttatgcctg gcgaattgcc
    43801 tgttcaacct caaagcgatt cgtcactttg ctgtcgattt tcacataagc tgaatgttct
    43861 tcttctgcaa tcaacacttc tttgatgcct tcagtttcca gcaaacgcac ttttaacgcc
    43921 tcgtttgcgg caatgttcgc cgggatttca atgcgcaagc tgctgacata cggcggttct
    43981 ttcatggtac tggcgactgc cagccacact gcggccagca ttgcgccagc gagaaatact
    44041 ccctgaccgt caaacatgcc gtcaatccag ccgcccagcg aaccgccaat cgccacgcca
    44101 agaaactggc tggtggagta aacacccatc gccgtacctt tgtaacctgc tggcgattct
    44161 ttactgataa gtgaaggcag aagggcttcc atcaaattaa acgccacaaa gaaaagctgc
    44221 acgccgacca ccagttgcca gaactgcgtt tgtgcgttcc acaacacaat ttccgcaacc
    44281 acgatcaacc cgacgcagaa gacaaagact tgcttcattt tgcgcttaac ttcagcgtag
    44341 ataatgaaag gcacgaccga gccaaaggcg attaacatcg ttgccaggta gaccttccag
    44401 tgttcagccg ccgggaaccc cgcatcagcc atctgtccgg gcagggcaac aaaggtcgac
    44461 atcagcaaaa tatgcagaca cataatgcca aagttgagtt tcagcagccg cggttccgcc
    44521 agcactttac tgaaactgcc ttttaccatt ccggactcac gattaagtac atgagtgcta
    44581 ctgttgggca caacccaaat ggtcaacgca atgccggttg ttgccagaat agcgatcatc
    44641 cagaacagca cgtgcagccc aagtttgtga gtgatgatcg ggccaagcac catcgcaatg
    44701 gcaaaggtaa tgccaaagct cacgccgata aacgccatcg ctttggtgcg gttttgttcg
    44761 cgcgtgagat cggaaagcag cgccataacg gctgcggcaa tcgcaccgga gccttgtagc
    44821 gcccggccca gaataattcc ccagatggag tcagagagcg cagcgataac gctaccggcg
    44881 gcaaacaccg ccagcccacc gacaattaat ggtttgcgac caatgcggtc tgaaagcagg
    44941 ccaaacggaa tctgaaaaac ggcctgagtc agaccataaa taccaatggc aataccgatt
    45001 aatgcttcgc tggcaccttg cagagccatg ccgtacgtgg tcagaaccgg cagaaccatg
    45061 aacatgccca gcatgcgtaa tgaaaatacg gtccctaaac cccaggtcgc gcgccgctca
    45121 cctggcgtca ttttataatc gttcattacc acctctgttt taaattcgcg actagtgtaa
    45181 agcggcaaac gaatggggta aacatgcgga ttttttagta aatatttcga gagggatatg
    45241 attctaatgc taagaaaaaa ggtgccgtag caccttttta actgagaggt tgtgttacca
    45301 cacagcagcc agcagcgtat gcgagtccgg caccataaaa tcaacggaca tcatcaccga
    45361 gagggcagtg atagcgatga tagagaaacc gaacagcttg cgcgcccaga ttctgtcatc
    45421 agcaacttta tagccgcgca gagccatgcc taaccaccag acgctcaccg cagcggcgac
    45481 caccagatat ttatacccag cgtagccgcc aagagagagc atcagcgtgg caacggcaaa
    45541 ggcgatgata tacagcgtga tgtgattctt cgccaccgaa atgcctttca ccaccggcag
    45601 taccggaatg tttgccgcct gataatcctt aaagcggaaa atggctatgg cataagagtg
    45661 aggcatctgc cacaggctga agatagccaa caggatcgct gcgccgctat cgaactcgcc
    45721 ggttaccgca cagtaaccga tcaccggagg cgcagcgccg gagagcgaac caatcaatgt
    45781 gccgtagaca gaatgacgtt tcatgtacag gctataaacg ccgacataaa ccacaaagcc
    45841 catcaccccc agccagcagg ccagcggatt cgcgccaaac cacagcagca taaagccagc
    45901 aatacccagc aaggtggcgt acaccagcga gacagcagga gagatcaggc ctttaaccag
    45961 cacccgattc ttcgtccttt ccatctttct gtcgatatcc ctgtcgatgt agttgttaaa
    46021 cacacaaccc gacgccacaa ccagtgacac cccaaccagc gtgtagataa acaggggata
    46081 atcaatgctg ccctttgagg ccagcaagaa tcccccaatc accgagatca ggttgccaaa
    46141 gatgatgcct ggtttcgtta cttgcaggta ttgcttaaat atcataaccg ccgctcttag
    46201 tgcatcatca tgttgtagtt gaggttccac ataatccaga tggagcccac aaccaggatg
    46261 gcgatgatta gcacggtgaa gacaaacgcc gtcatattcc agccttcatc tgatttggta
    46321 ttcatgtgca ggaagcacac cagatgcacc agaatttgta ccactgccat tgccaggatt
    46381 gttcccagaa ttacggccgg agaggcagcc cctgtcatca ccatccagaa cggaattacc
    46441 gtcaggatta tcgacaggat aaagcctgtc atgtaggtct ttacgctgcc atgggatgcg
    46501 ccgctgcttg cgttagaatg actcattaca tcgcccccat cagataaaca acagtgaaca
    46561 cacagatcca aaccacatcc aggaagtgcc agaacaggct caggcacatg atgcgggtac
    46621 ggttagtgct ggtcaggccg cgacgggcga tttgcaccat cagcaccgcc atccagataa
    46681 gaccggaagt gacgtgcaga ccgtgcgtgc cgaccagcgc aaagaatgct gacaggaagc
    46741 cgctgcgatc cggacccatg ccgttaacaa tcaggtgatg gaattcatag atttccatcc
    46801 cgataaatcc ggcaccaaac aaccatgtca acgccagcca ggagatcacc tggcttttgt
    46861 tgtttttgta catggcgata gccgccatgc cataggtgat ggagctgaac aacagcaaga
    46921 aagtttcaac cagaacgaac ggcagttcga aaatgtcctt acctgtcggg ccgcctgcgg
    46981 tgccgttcac cagaacggca taggtagcaa acaagataga gaacagaatg cagtcgctca
    47041 tcaggtagat ccaaaatccg aaaattttgg ttccgcctgc atcgtggtgc ccgtgttcgt
    47101 gcgcgtgggc agtcgcgtgc gtcaaagtat cagttgccat ttttcagccc tgccttagta
    47161 atctcatcga aatgctggtt ttccagtttt tcgatttctg ccaccggcac gtagtaatcc
    47221 acgtcctcgt cgaagctttt cacgatccag gtgatgatca tgcctgcgaa gccaacaatc
    47281 gctagccacc agatatgcca gatcatggcg aaaccgaaga tggtggagaa agccgcaatg
    47341 acgataccgg caccgctgtt tttcggcata tgaatttctt catagtggtc aggctttttg
    47401 tacgcttcgc ctttctcttt catttcccag aatgcatcac gttcgtgaac gtgcggcaca
    47461 acggcaaagt tatagaacgg aggcggggaa gaggttgccc actccagcgt acggccaccc
    47521 cacgggtcgc cagtcaggtc acggttctgg tcgcggtcac gaatagaaac gtatatctga
    47581 ataacgaggc agagaatacc cagcgcaatc agtaccgcac cgctggctgc aatcatcagc
    47641 atggtgtgga actgcgggtc aatctgctgg ctcaaacgac gggtcatgcc catgaagccc
    47701 agcgcataca gtggcataaa ggcaacgaag aagccgatga tccagaacca gaacgcgcgt
    47761 ttaccccagg tttcgttcag tttgaaaccg aacgctttag gccaccagta ggtcatccct
    47821 gcgaagcagc cgaagaccac gccgccgatg atcacgttat ggaagtgcgc aatcaggaac
    47881 aggctgttat gcagaacgaa gtccgcgccc ggtactgcca gcagcacgcc agtcataccg
    47941 cctaccgaga aggtaacgat aaaaccgatg gtccacagca tcgcagaatg gaacacgatg
    48001 cggccctgat acatggtgaa cagccagttg aagatcttca ccccggtagg gatggcgata
    48061 atcatcgtgg tgataccaaa gaaggcgttt acgttcgcgc ccgcacccat cgtaaagaag
    48121 tggtgcagcc agacgatgaa cgacagcacg gtgatacaga cggttgccca taccagcgag
    48181 gtataaccaa acagacgttt acgcgagaag gttgccgcaa tttcggagaa cacaccgaat
    48241 acaggcagga tcaagatgta aacttccggg tggccccagg cccaaatcag gttgatatac
    48301 atcatcatgt tgccacccat atcgttggta aagaaatggg tgcccagata gcgatccagg
    48361 gtcaacaacg cgacggtaac cgtcagaatt gggaaggaag caataatcag tacgtttgcg
    48421 cacagtgatg cccaggtaaa tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc
    48481 ttcagaatgg taacgaagaa gttgataccg gtaagcgtcg tacctatacc ggatagctgg
    48541 agactccata tccagtaatc gacaccgact cccggactgt actctattcc cgatagcggt
    48601 ggataggcca gccagccggt ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga
    48661 atcacaccaa caacggtaaa ccagaagctt aagttgttga ggaacgggaa cgcaacgtca
    48721 cgcgcgccga tctgcagcgg aaccaccagg ttcatcagac cgataacgaa aggcatcgcc
    48781 acgaagaaga tcatgatcac gccgtgtgcg gtgaagatct gatcgtagtg gtgaggtggc
    48841 aggaagcccg cttcgcccgc agaggcaagg gcctgctgac tacgcatcat aatggcgtca
    48901 gcaaaaccac gcagcaacat cacaatcgcc acgatgatat acatgatacc gagacgttta
    48961 tggtcaacgg aggtcagcca ctctttccac aggtaggtcc acttaccgaa gtaagtgatc
    49021 aggccaacga gcgccagacc tcccaaaata atgccagcga tcgtaaccat gacgataggt
    49081 tcatggaacg ggactgcatc aagtgataat tttccgaaca tctttattct tcctcaaccc
    49141 ctttaatggg cggattccgc gtggctcatg tccatgcctt ccataccttc atgtgcgctg
    49201 tgctcacctt ctggctgggt catgtccatg ctcttaccgt gagccataaa cttgttaatc
    49261 acatcggcaa acaagtctgg tttcacgttg gagaaatatt ccacctggtt gtattcgcta
    49321 ggcgcggcca gtttttcgaa cgctgccatg tcagacatgc tgttcggcga ctgtttcgct
    49381 tttgcgaccc attggtcgaa tgcggcgcga tccggtgttg caatagcttt gaacttcatg
    49441 cctgagaagc ccgggccgct gtagctggcg gagataccgt cataagtacc gggttcgttg
    49501 gcgatcagat gcaggcgagt ctgcataccg gccatggcat aaatctggct acccagacgc
    49561 ggaatgaaga aggagttcat cacggagttg gaggtcactt tgaagtacac cggagtgttc
    49621 gccgggaaag cgatttcatt cacggttgca atgccctgtt ccgggtagat gaagaaccat
    49681 ttccagtcca tggaaaccac ttcgatggta atgggcttct cgtcgtgtgc cagcggcttg
    49741 ctaggctcaa gagcgtgagt ggttttccag gtcagtaccg caaggaagat gatgattaag
    49801 ataggtaccg tccagaccac agcttccact ttattggagt gtgaccagtt cgggctgtac
    49861 ttagcatctt tattgctcgc acggtacttc caggcgaaac caacagccat caagattgcg
    49921 ggaataacga caatcaacat caggccaaat gccgtcagta tcagtgaacg ttgctccaga
    49981 ccaatctgtc ctttgggatc taacagcgca gaattacagc cactgagcaa tacagtgcct
    50041 gcaaataatg acaaccatcc caaactttta ttgtatttcc tgagtctcat ttaacgacct
    50101 caattccacg ggatctggtg gcgtttaaag tgtgtgggca ttttacggga aggttacatt
    50161 actgtaaaca tgattaaatt cctgttactt ggatttggct atcaggtcac atatgttgca
    50221 aaacatatca agttgatttt aagaagctgt tgcaaaaggg cgttataacg aaagggaagt
    50281 gcagaaggag tttgggcggg caattggtat aatcaatata aaataaaaca attatttaac
    50341 aaataactat cattagggtg acaactgggt ggtgagaaaa taaggactca tcagctgaat
    50401 cgtgtaaaaa aaatagaatt cataaatggc gtcaataatt tccagacgct aagccgcaca
    50461 aaagaacaaa tatcaataag cgatgctgat gtttatttag ccgtaataat tacggcgagt
    50521 gattactaca gctaaataat atttacagat tacgtcagat gtgtttttcg tagcgccaga
    50581 taatccagca aaccaccaag cacgacgcca gagagggcga ctaaaacccc gacttccagc
    50641 agcgcaggca ggaaagagaa gtgcgtcaaa tccagcgcgt ccatcgtcaa cagcagtaac
    50701 cacacggcca acaggctgat gcccgccgcc agtgtccaca tggcaaaggc ataacctgca
    50761 ggatattcgg tacgggagat aaagttgtca tttactcgtg tatattcaag cgtctggcgg
    50821 caaaccagca gcaaaataag ccctggtacg gcagcggcga cggagaatag atagaatgtc
    50881 gaccagccgt gtgcttcaac aaaccaaccc gccacggggc cgacataaac tcgccctaca
    50941 gcagaaagcg ctgagagcag ggcaaattga gtagcggaaa atgacttatt acacagcgtc
    51001 attaacagcg cgacaaaggc tgatgtaccc atcccgccac agaggttttc gaaaaagacg
    51061 gctgcaccca tgctgtagag atgcttatca gtaatcgaca gcagccagta accggcgtta
    51121 gatgcacctt gtaaaatgcc gaaaatcagc agtgcacgga acagcgacag gcgctgcatc
    51181 aaaatcccac cgtacaatgc gccaacaatg gtcgccagta agccaagcgt tttgttaacc
    51241 acgcctactt cacccgcatc aaacccgacg ccgcgaatca aaaacgtggt tgtcaggctc
    51301 atagcgaatg cgtcgcccag cttatacagc acgataagaa gcaaaataag ccaggcatta
    51361 ttgcgaccaa agaaatctcg cagaggtgca acaaccgctt gttccagcgt tttgggcaca
    51421 ggaatggtgt cggtgggttc tggtgcaagc aacgtcgcga taatacaggg gatcaacagc
    51481 gccgccatca accagtacat gccctgccag cccagccatt tatctgccag ccacagggct
    51541 aagccgccgg aaaccagcat ccctaaacgg taacccagca cgctgattgc cgcgcccgca
    51601 ccacgttctt ctgccgggag cacatcggtt ttccacgcat cgaagacaat atcctgggag
    51661 gcagagcaaa aagcgatcac caccgccagt gccgccatcc agcggagctg ggtgcctggt
    51721 tcgagaaaac ccatcgccgc aatggcgact aataacagaa tttgcgtggc gagcagccaa
    51781 ccgcgccgcc gcccgaaaaa tgggggcgtg tagcggtcca tcagtggtga ccagaggaat
    51841 ttaaaaacgt aagcctggcc taccagagag aagaaaccaa tggttttgag atcgatattc
    51901 tcgaccgtca tccaggcctg taaggtgccg gacgtcaggg cgagcggtag cccggaagca
    51961 aaacccagga tcagcaatat ggctgaacgc ggctgttgaa aaatacgtaa atattgactg
    52021 gacatgggcg tctacaggcc cggcttgcac cgggccagag ggcagaaatt aacgcgcgtt
    52081 ctgcttgatg aattcgtgga tgctggtgtc ctgagacata tcagcgatgg tatcggtcag
    52141 cacactgtta accgcatcgg cgatattttt gttggaggcc tggaacgcac cttcaacgtt
    52201 gtagctggca cgatagtttt tggtcatttt gttgccattc tgcgcggtag cgatgatggc
    52261 gatatccgct ttggtcgcga tgttgtagcg cacgttgccc tgggacacgt cagcatacag
    52321 ttggctaacg atgatttgca gattaaccgg gccattcgga ccaaccatgt atccacgggc
    52381 ggtcatttgt ttttccagca cttcttgcag caggaaacgc agatcgcggg aggcggtcag
    52441 ggtaacgatt tgattatcgc gggtgacttt tgccagcgcc tgatcggtac gctgatcggc
    52501 accattaatg cttacggtga cgcccatcag gcttggatcc tgctgtggca gtgtaatcgt
    52561 cggggaaact tcgatagttg ttggcggttt tgcgcatcct gcaagcataa acagagcaac
    52621 taacgggaag aggatttttt tgaacatgtt cgggctctca gagactctta agcgtgtttg
    52681 gtaaaaattc ccgccatcat aacattgcca acggcgaggg gaagtgggta aggcatgtaa
    52741 attcatcatg ttgacgaaat aatcacccct ggtaaaagaa acgctgatgc gaggcctgtg
    52801 tttcaatctt taaatcagta aacttcatac gcttgacgga aaaaccagga cgaaacctaa
    52861 atatttgttg ttaagctgca atggaaacgg taaaagcggc tagtatttaa agggatggat
    52921 gacatctcag cgttgtcgga ggagatattt catgatgata cgtgagcgga tagaagaaaa
    52981 attaagggcg gcgttccaac ccgtattcct cgaagtagtg gatgaaagct atcgtcacaa
    53041 tgtcccagcc ggctctgaaa gccattttaa agttgtgctg gtcagcgatc gttttacggg
    53101 tgaacgtttt ctgaatcgtc atcgaatgat ttacagtact ttagcggagg aactctctac
    53161 taccgttcat gcgctggctc tgcatactta cactattaag gagtgggaag ggttgcagga
    53221 caccgtcttt gcctctcctc cctgtcgtgg agcaggaagc atcgcgtaaa aacgcatttg
    53281 caactgtcgg cgcttttcca gtatgttgct aaagatttta tgaaaaacgg cctgcgggcc
    53341 gttttgtttt gtctggattt tgcgcttttt gcccagcatt cagacgaaaa tcgcccggga
    53401 attgtgaaaa aatacgcgac agcgcgcaat aaccgttctc gactcataaa agtgatgccg
    53461 ctataatgcc gcgtcctatt tgaatgcttt cgggatgatt ctggtaacag ggaatgtgat
    53521 tgattataag aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg ggtaagagtt
    53581 gaccgagcac tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa accactcaag
    53641 gccttggccg ccgtgtaacg attactatcg ctgctgacag catcgagacc gctgttaaaa
    53701 gcgagctggt caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa ggcaaagtgc
    53761 caatgaatat cgttgctcag cgttatggcg cgtctgtacg ccaggacgtc ctaggcgacc
    53821 tgatgagccg taacttcatt gacgccatca ttaaagaaaa aatcaatccg gctggcgcac
    53881 cgacctatgt tccgggcgaa tacaagctgg gtgaagactt cacctactct gtagagtttg
    53941 aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa aaaccgattg
    54001 ttgaagtgac cgacgctgac gttgacggca tgctggatac tctgcgtaaa cagcaggcaa
    54061 cctggaaaga aaaagacggc gctgttgaag cagaagaccg tgtgaccatc gacttcaccg
    54121 gttctgtaga cggagaagag ttcgaaggcg gtaaagcgtc tgatttcgta ctggcgatgg
    54181 gtcagggtcg catgatcccg ggctttgaag acggtatcaa aggccacaaa gctggcgaag
    54241 agttcaccat cgacgtgacc ttcccggaag agtaccacgc agaaaacctg aaaggtaaag
    54301 cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg gaactgactg
    54361 aagaattcat caaacgtttc ggcgttgaag atggttccgt agaaggtctg cgcgctgaag
    54421 tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg taaccgcgtt aagtctcagg
    54481 cgatcgaagg tctggtaaaa gctaacgaca tcgatgtacc ggctgcgctg atcgacagcg
    54541 aaatcgacgt tctgcgtcgc caggctgcac agcgtttcgg tggcaacgaa aaacaagctc
    54601 tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg ccgcgtagtt gttggcctgc
    54661 tgctgggcga agttatccgc accaacgagc tgaaagctga cgaagagcgc gtgaaaggct
    54721 tgatcgaaga gatggcttct gcgtacgaag atccgaaaga agttatcgag ttctacagca
    54781 aaaacaaaga actgatggac aacatgcgca atgttgctct ggaagaacag gctgttgaag
    54841 ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag ctgatgaacc
    54901 agcaggcgta atttacgtag cataacgcgc taaattcgca caaaggcccg tcaccgccag
    54961 gtggtgggct tttttttgtc atgaattttg catggaaccg tgcgaaaagc ctctttcggt
    55021 gttagcgtaa caacaaaaga ttgttatgct tgaaatatgg tgatgccgta cccataacac
    55081 agggactagc tgataatccg tccataaggt tacaatcggt acagcaggtt ttttcaattt
    55141 tatccaggag acggaaatgt catacagcgg cgaacgagat aactttgcac cccatatggc
    55201 gctggtgccg atggtcattg aacagacctc acgaggtgag cgctcttttg atatctattc
    55261 tcgtctactt aaggaacgcg tcatttttct gactggccag gttgaagacc acatggctaa
    55321 cctgattgtg gcgcagatgc tgttcctgga agcagaaaac ccagaaaaag atatctatct
    55381 gtacattaac tctccaggcg gggtgattac tgccgggatg tctatctatg acaccatgca
    55441 gtttatcaag cctgatgtca gcaccatctg tatgggccag gcggcctcga tgggcgcttt
    55501 cttgctgact gcaggggcaa aaggtaaacg tttctgcctg ccgaattcgc gcgtgatgat
    55561 tcaccagccg ttgggcggct accagggcca ggcgaccgat atcgaaattc atgcccgtga
    55621 aattctgaaa gttaaagggc gcatgaatga acttatggcg cttcatacgg gtcaatcatt
    55681 agaacagatt gaacgtgata ccgagcgcga tcgcttcctt tctgcccctg aagcggtgga
    55741 atacggtctg gtcgattcga ttctgaccca tcgtaattga tgccagaggc gcaactgtgc
    55801 cgctatactt atccagggcg gcacaacgct gtaagcggct tgcgcctgag aatggcattt
    55861 gcgtcgtcgt gtgcggcaca aagaacaaag aagaggtttt gacccatgac agataaacgc
    55921 aaagatggct caggcaaatt gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg
    55981 cgcaagctga ttgccggtcc atccgtgtat atctgcgacg aatgtgttga tttatgtaac
    56041 gacatcattc gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg
    56101 acgccgcatg aaattcgcaa ccacctggac gattacgtta tcggtcagga acaggcgaaa
    56161 aaagtgctgg cggtcgcggt atacaaccac tacaaacgtc tgcgcaacgg agataccagc
    56221 aatggcgtcg agttgggcaa aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa
    56281 acgctgctgg ccgaaacgct ggcgcgcctg ctggacgttc cgttcaccat ggctgacgca
    56341 accacgctga ccgaagccgg ttatgtgggc gaagacgttg aaaacatcat tcagaagctg
    56401 ttgcagaagt gcgattacga cgtacagaaa gcgcagcgcg ggattgtcta catcgatgaa
    56461 atcgacaaga tttctcgtaa gtcagacaac ccgtccatta cccgagacgt ttccggtgaa
    56521 ggcgtacagc aggcactgtt aaaactgatc gaaggtacgg tagctgctgt tccgccgcaa
    56581 ggtgggcgta aacatccgca gcaggaattc ttgcaggttg atacctctaa gatcctgttc
    56641 atctgtggcg gcgcatttgc tggcctggat aaagtgattg ccaatcgtgt tgaaaccggc
    56701 tccggcattg gttttggcgc gacggtgaaa gccaagtccg acaaagccag cgaaggtgaa
    56761 ctgctggcgc aggttgaacc ggaagatctg atcaagtttg gtctgatccc tgagttcatt
    56821 ggtcgtctgc cggttgtcgc aacgttgaat gaactgagcg aagaagctct gattcagatc
    56881 ctcaaagagc cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa tctggaaggc
    56941 gtggatctgg aattccgtga cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt
    57001 aaaaccggtg cccgtggcct gcgttccatc gtagaagccg cactgctcga taccatgtac
    57061 gatctgccgt ccatggaaga tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt
    57121 caaagcaaac cgttgctgat ttatggtaag ccggaagcgc aacaggcatc tggtgaataa
    57181 ttaaccattc ccatacaatt agttaaccga aaagggggga ttttatctcc cctttaattt
    57241 ttcctctatt ctcggcgttg aatgtggggg aaacatcccc atatactgac gtacatgtta
    57301 atagatggcg tgaagcacag tcgtgtcatc tgattacctg gcggaaatta aactaagaga
    57361 gagctctatg aatcctgagc gttctgaacg cattgaaatc cccgtattgc cgctgcgcga
    57421 tgtggtggtt tatccgcaca tggtcatccc cttatttgtc gggcgggaaa aatccatccg
    57481 ttgtctggaa gcggcgatgg accatgataa aaaaattatg ctggtcgcgc agaaagaagc
    57541 ttcaacggat gagccgggtg taaacgatct tttcaccgtc gggaccgtgg cctctatatt
    57601 gcagatgctg aaactgcctg acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc
    57661 gcgtatttct gcgctctctg acaatggcga acacttttct gcgaaggcgg agtatctgga
    57721 gtcgccgacc attgatgagc gggaacagga agtgctggtg cgtactgcaa tcagccagtt
    57781 cgaaggctac atcaagctaa acaaaaaaat cccaccagaa gtgctgacgt cgctgaatag
    57841 catcgacgat ccggcgcgtc tggcggatac cattgctgca catatgccgc tgaaactggc
    57901 tgacaaacag tccgttctgg agatgtccga cgttaacgaa cgtctggaat atctgatggc
    57961 aatgatggaa tcggaaatcg atctgctgca ggttgagaaa cgcattcgca accgcgttaa
    58021 aaagcagatg gagaaatccc agcgtgagta ctatctgaac gagcaaatga aagctattca
    58081 gaaagaactc ggtgaaatgg acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat
    58141 cgacgcggcg aagatgccga aagaggcaaa agagaaagcg gaagcagagc tgcagaagct
    58201 gaaaatgatg tctccgatgt cggcagaagc gaccgtagtg cgtggatata tcgactggat
    58261 ggtacaggtg ccgtggaatg cgcgtagcaa ggtcaaaaaa gacctgcgtc aggcgcagga
    58321 aatccttgat accgaccatt atggtctgga gcgcgtgaaa gatcgtatcc ttgagtatct
    58381 tgcggttcaa agccgtgtca acaaaatcaa gggtccgatc ctctgcctgg tagggccgcc
    58441 gggggtaggt aaaacgtccc tgggtcagtc catcgccaaa gccaccgggc gtaaatatgt
    58501 ccgtatggcg ctgggcggcg tgcgtgatga agcggaaatc cgtggtcacc gccgtactta
    58561 catcggttct atgccgggta aactgatcca gaaaatggcg aaagtgggcg tgaaaaaccc
    58621 gctgttcctg ctcgatgaga tcgacaaaat gtcttctgac atgcgtggtg atccggcctc
    58681 cgcactgctt gaagtgctgg atccagagca gaacgtagcg ttcagcgacc actacctgga
    58741 agtggattac gatctcagcg acgtaatgtt tgtcgctacg tcgaactcca tgaacattcc
    58801 ggcaccgttg ctggatcgta tggaagtgat tcgcctctcc ggttataccg aagatgaaaa
    58861 actgaacatc gccaaacgtc acctgctgcc gaagcagatt gaacgtaatg cattgaaaaa
    58921 aggtgagctg accgtcgacg atagcgccat tatcggcatt attcgttact acacccgtga
    58981 ggcgggcgtg cgtggtctgg agcgtgaaat ctccaaactg tgtcgcaaag cggttaagca
    59041 gttactgctc gataagtcat taaaacatat cgaaattaac ggtgataacc tgcatgacta
    59101 cctcggtgtt cagcgtttcg actatgggcg cgcggataac gaaaaccgtg tcggccaggt
    59161 aaccggtctg gcgtggacgg aagtgggcgg tgacttgctg accattgaaa ccgcgtgcgt
    59221 tccggggaaa ggcaaactga cctataccgg ttcgctcggc gaagtgatgc aggagtctat
    59281 tcaggcggcg ttaacggtgg ttcgtgcgcg tgcggaaaaa ctggggatca accctgattt
    59341 ttacgaaaaa cgtgacatcc acgtccacgt accggaaggt gcgacgccga aagatggtcc
    59401 gagtgccggt attgccatgt gcaccgcgct ggtttcttgc ctgaccggta acccggttcg
    59461 tgccgatgtg gcaatgaccg gtgagatcac tctgcgtggt caggtactgc cgatcggtgg
    59521 tttgaaagaa aaactactgg cagcgcatcg cggcgggatt aaaacagtgt taattccgtt
    59581 cgaaaataaa cgcgatctgg aagagattcc tgacaacgta attgccgatc tggacattca
    59641 tcctgtgaag cgcattgagg aagttctgac tctggcgcta caaaatgaac cgtctggcat
    59701 gcaggttgtg actgcaaaat agtgacctcg cgcaaaatgc actaataaaa acagggctgg
    59761 caggctaatt cgggcttgcc agcctttttt tgtctcgcta agttagatgg cggatcgggc
    59821 ttgcccttat taaggggtgt tgtaagggga tggctggcct gatataactg ctgcgcgttc
    59881 gtaccttgaa ggattcaagt gcgatataaa ttataaagag gaagagaaga gtgaataaat
    59941 ctcaattgat cgacaagatt gctgcagggg ctgatatctc taaagctgcg gctggccgtg
    60001 cgttagatgc tattattgct tccgtaactg aatctctgaa agaaggggat gatgtagcac
    60061 tggtaggttt tggtactttt gccgttaagg aacgtgctgc ccgtactggc cgcaacccgc
    60121 agaccggtaa agagatcacc atcgctgctg ctaaagtacc gagcttccgt gcaggtaaag
    60181 cactgaaaga cgcggtaaac taagcgttgt ccccagtggg gatgtgacga agttcaaggg
    60241 cgcatctact gatgtgcctt ttttatttgt attcggtgac tttctgcgtc ttgtgggctg
    60301 acaattgccc ccgtttcttg tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg
    60361 tacacagtca tctacagcgg agtgttgtta caccatgatg gacagcttac gcacggctgc
    60421 aaacagtctc gtgctcaaga ttattttcgg tatcattatc gtgtcgttca tattgaccgg
    60481 cgtgagtggt tacctgattg gcggaggcaa taactacgcc gcaaaagtga atgaccagga
    60541 aatcagccgt gggcaattcg agaacgcctt caacagcgag cgtaatcgca tgcagcaaca
    60601 gctgggcgat caatactccg agctggcagc gaacgaaggc tatatgaaaa ccctgcgtca
    60661 acaggtgctg aatcgtctga tcgacgaggc gcttctggat cagtacgctc gtgagctgaa
    60721 actgggtatc agcgatgagc aggttaaaca ggcgattttc gcgaccccag ccttccaggt
    60781 tgacggcaaa tttgataaca gccgctataa cggtatcctc aaccagatgg ggatgaccgc
    60841 cgatcagtac gcccaggcgc tgcgtaacca gctcactacc caacagctga ttaacggcgt
    60901 tgccggtacc gattttatgc tgaaaggtga aaccgacgag ctggcggcac tggtagctca
    60961 acaacgcgtg gtgcgtgaag cggctatcga tgttaacgcg ctggcggcga agcagcctgt
    61021 gaccgaacag gaaattgcca gctactacga acaaaacaaa aacaatttca tgacgccgga
    61081 acaattccgc gtgagttaca tcaagctgga tgccgcgacg atgcagcaac cggttagcga
    61141 tgcggatatc cagagctact acgatcagca tcaggatcaa ttcacccagc cgcagcgtac
    61201 ccgttacagc atcatccaga ccaaaactga agatgaagcg aaagcggtac ttgatgagct
    61261 gaataagggc ggtgattttg ctgcattagc caaagaaaaa tctgccgata ttatctctgc
    61321 ccgtaacggc ggtgatatgg gttggttaga agatgccact atcccggacg aactgaaaaa
    61381 tgcgggtctg aaagaaaaag gccaactgtc tggtgttatc aaatcttcgg tcggtttcct
    61441 gattgtacgt ctggacgaca ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga
    61501 cgacattgcg gcgaaagtga aacacgaaaa agccctcgat gcgtactacg cgctgcagca
    61561 gaaagtgagc gatgcggcaa gcaacgacac cgagtctctg gccggtgcag agcaagctgc
    61621 cggcgttaaa gccactcaga cgggttggtt cagcaaagat aacctgccgg aagagttgaa
    61681 cttcaagcca gttgccgacg ctatcttcaa cggcggtctg gtaggtgaaa acggcgcgcc
    61741 gggcatcaac tctgacatca tcaccgtaga cggcgaccgc gcattcgtgc tgcgcatcag
    61801 cgagcacaaa ccggaagcgg tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact
    61861 ggttcagcac aacaaagctg aacaacaggc gaaagtggat gctgagaaac tgctggttga
    61921 tttgaaagcc ggcaaaggtg cggaagcgat gcaggctgcc ggtctgaaat ttggcgagcc
    61981 gaaaacctta agccgttccg gtcgtgaccc gattagccag gctgcgtttg cactgccgct
    62041 gccagcgaaa gacaaaccga gctacggtat ggcgaccgat atgcaaggta atgtggttct
    62101 gctggcgctg gatgaagtga aacaaggttc aatgccggaa gatcagaaaa aagcgatggt
    62161 gcagggtatc acccagaaca acgcacaaat cgtctttgaa gctctgatga gtaacctgcg
    62221 taaagaggcg aaaatcaaaa ttggcgatgc gctggaacag caataatcct gaagccgcct
    62281 cgcaaaaaaa tgctttgcag ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt
    62341 ctgaacattg ccatttgttt actgttttca ctgccgttaa ggtgattcca ctgttaacaa
    62401 acaaggagaa aacagtatga aacacggaat taaagcactg ctcattaccc tgtccctggc
    62461 ctgtgccgga atgtctcata gtgctctggc ggcagctcct gcggcaaaac cgacaacggt
    62521 agaaaccaaa gcggaagctc ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag
    62581 tgacgaagaa ggcacccggg tcagcattaa taatgccagc gcggaagagt tagcccgcgc
    62641 gatgaatggc gttggcctga agaaagcgca ggcgattgtc agttatcgcg aagagtacgg
    62701 tccgtttaaa acggtggagg atctaaagca ggtgccgggg atgggcaatt cgctggtgga
    62761 acgtaatatg gcggtattaa ccctgtaatt aatttgcata gtggcaattt tttgccagac
    62821 tgaagaggtc ataccagtta tgacctctgt acttataaca gttcttataa cagttcttat
    62881 aacagttctt ataacaacaa cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg
    62941 tggatatcat ctcgacgttt accagcacgt aaacaacgcc cgctaccttg aatttctcga
    63001 agaagcccgc tggcatgggt tggaaaatag cgacagtttt cactggatga cggcccataa
    63061 catcgctttc gtcgtggtca atatcaatat taactatcgc cgcccggcgg tattaagcga
    63121 cctgttaacc attaccagtc agttgcagca attaaacggt aaaagtggca tcttaagcca
    63181 ggtcattaca ctggagccgg aaggacaggt ggtagcggat gcgcttatta cgtttgtttg
    63241 tattgatctt aaaacgcaga aagcattagc tctggaaggg gaattgcgcg aaaagctgga
    63301 gcagatggtt aagtaaacgt tttgtggtgc cggatgctca agccgcatcc ggcgacaccc
    63361 ggaataatta cttcaacccg gttttctgct tcatcgctgc catcaccgtc ggtttatcgg
    63421 ccagataatg attcaaaccg ttggcacgta aattacacgc cgcacaatga ccgcaaccgt
    63481 cgcctttaat gccgttataa caggtcagcg tttcgttacg gactaaatcc agcttgccgt
    63541 aataatctgc cagcgcccag gtttccgctt tatcaatcca catcagcggc gtttcaaaac
    63601 gaatatcttt cgccatgccc aaactgacgg catggtttag tgctttcaca aactcatcgc
    63661 gacaatccgg gtagccggag aaatccgttt cgcagacgcc ggtaattacg gcttctgctt
    63721 ttacctgata cgcatatatt gccgccagcg tcaggaacaa aatattacgc cctgggacaa
    63781 acgtattcgg gataccatcg gcttcaggtt cataatcagg caccggaatg ctgtcacgcg
    63841 tcaggctgct gaccgccagc tcgttgagca gcgttacatc aagaacctta tgcgcgcgtg
    63901 cccccagttt cagcgccagt tcgcgtgcca cgtcgatttc tgcgcgatgg cgttgaccgt
    63961 aatcgaacgt cacgcaatgg acttcatcat attgttgtaa tgcctgcacc agacaggtgg
    64021 tggaatcctg gcctccactg aacacaacga cagcacgttt catagataat cctgcttgaa
    64081 caataaaagc gttatggtaa cgcctgcgat taacccggac cagctattca ttgcgacggc
    64141 gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc
    64201 acgccgggag gggcgctaat gcgatagtga tagttgaaca gcggcgtcag cgtcgcatct
    64261 gccattaact ggctaaaaac cgctttcagg gcattaaatc ggttttcctc atcaggcatt
    64321 acttgcactg cgtccagtgt cgattgcaaa tgtgcatatg ctggagcgtc gaaaacatgt
    64381 ggccacagtg gatcgcaacg cagccattgc tccagggtat attccggtgc ttcgccaatt
    64441 aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt
    64501 gcgttatgaa aaataattgt gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt
    64561 tctgccatgg tatgaagttc tatcggtagg tgataaacca gcgtcaaggt tttcggtagt
    64621 ttaacttcat ccggtacctg ccaatgcgga atagtccagc caggcagtaa tgcatgactg
    64681 gcggtgatca ggttttctcc gacttctaac gtttgtaata aaccggattg atgaataatg
    64741 gagattactt ttcgcgcctg ccagagggac agtcggagac ttttgcgcaa tgttaaatag
    64801 caaaaaccta aactgatgcc gctactgacc tggctgaccc gttgcagctc ctccggtttg
    64861 ccaatggtga tttgcacggg atgccgacaa ctggttccca aatctttttc gaaaagcggc
    64921 ggagttatcc agtactcaac cgctttaagc agcggatgac gtaaatggta ataatcatgg
    64981 ctctccaggc gcaccagttc cgctgtgaat tgtgttaagc gaaaaggacc tgtgccgatc
    65041 agtgggaatt gcggatgcgc cagatggctg caatagctcg ccagtcggtg cgcaagccag
    65101 taatcggggc gatgtaaaaa gaaggtcaga cactgcggat gggtgacttc aatacgcttc
    65161 acgctaataa ataattgatc cagtgctggc agttgtaaca gcatcaataa tcgctggtgt
    65221 aagtgtgagg cttttactgc atcgccgtta tgccagtgta gcgttgaacg aagataaaag
    65281 tcccagcgta acccgtcagt agaggtttcc cagtgatgtg ctaaatcgcc aatcgggcgc
    65341 tgagtattat tatcgaagcg ggtcaggccg gaaaatatct gcccggccag atgctgctcg
    65401 gcacggccgg gcaaaaagcc cggttgtagc ggttcgagcg ggcgatagta gggaatacgc
    65461 aacgtgggcg tatcgttttg ccattgtccg cccataaacg gctgtaacag agtgcgcagc
    65521 tcacctgggg ccagttgcgc cagctccagc acatcttgct gctttccggt ttccagcgcc
    65581 tgttccatca tcgcattgcg tagcgattct ggcgtgacca gaaagcgtag ttgaccgcgc
    65641 tttccgcgtc ctgactgcgc ctgccactcc agccatcccg cctcctgtgc ctgacgcaac
    65701 accgtacgaa catggcgttc gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg
    65761 gtttgcggct ttccggcgga aggttgccac agacgttgat actggttaag acggttgagc
    65821 agtcgcatat aaacccggaa caatattatt taactattca ctattacttc cgtatatatc
    65881 aggtgatact caatcaccat taactgtgtc acagagtgga gaaagaaatg gctcgtctgg
    65941 cagcatttga tatggatggc actttattga tgcctgacca tcatttaggt gagaaaaccc
    66001 tctctacttt ggcgcggctg cgtgaacgcg acattaccct cacttttgcc acggggcgtc
    66061 atgcgctgga gatgcagcat attctcgggg cgctatcgct ggatgcatat ttgattactg
    66121 gcaacggaac gcgcgtgcat tctctggaag gtgaactttt acatcgtgat gatttacctg
    66181 cggatgtcgc ggagctggtg ctgtatcagc aatgggatac ccgagcaagc atgcatatct
    66241 tcaatgacga tggttggttt accgggaaag agatccctgc gttgttgcag gcatttgtct
    66301 atagcggttt tcgttatcag ataatcgatg tcaaaaaaat gccactcggc agcgtcacca
    66361 agatctgctt ctgtggcgat cacgacgatc ttacacgctt gcagatccag ctatacgaag
    66421 cattaggcga gcgtgcacat ttatgttttt ccgccacgga ttgcctcgaa gtgctgccgg
    66481 tgggctgcaa taaaggcgct gcattgacgg tgctgaccca acatttaggt ttatcgttac
    66541 gcgattgtat ggcctttggt gatgcgatga acgatcgcga aatgttaggc agcgtcggta
    66601 gcggatttat tatgggcaat gcgatgccgc aactgcgcgc ggagctcccg catttaccgg
    66661 tgattggaca ttgccgaaat caggctgtct ctcactattt gacgcactgg ctggactatc
    66721 cacatctacc ttattccccc gaataacgag atcccttcca gcaccgggca attgcccggt
    66781 tttttttgcg ttgaatttgt cattttgtgc cgtggtgctt aaaccgcaca gaataaattg
    66841 tcgcgatttc acctttaaaa tagaattaaa agagaaaaaa attctctgtg gaagggctat
    66901 gttagataaa attgaccgta agctgctggc cttgctacag caggattgca ccctctcttt
    66961 gcaggcactg gctgaagccg ttaatctgac aaccaccccc tgctggaagc gtctgaaacg
    67021 gctggaggac gacggtattc ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg
    67081 cctcggcctg accgcctttg tgctgataaa aacgcaacat cacagcagcg aatggtattg
    67141 ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg gggttctggc gcatggctgg
    67201 tgaatacgat tatctgatgc gcgtccaggt tgccgacatg aaacgctacg acgagtttta
    67261 taagcgtctg gtaaacagtg taccggggct gtcggacgtc acttccagct tcgcgatgga
    67321 acagattaaa tacaccactt ctttacccat cgaataaata tccagaatca agtcaggaca
    67381 caacgcgtgc gattatttgc tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat
    67441 ctcggggctg tcgccttgct tgtcattatc gcgatgctgc aactggttcc gccaaaagtg
    67501 gttggtattg ttgttgatgg cgtgacagaa caacacttta ctaccgggca gatcctgatg
    67561 tggatcgcca ccatggtgct gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc
    67621 gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac tgcgtgaaga ttattaccgc
    67681 cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc gcacgggcga ccttatggcc
    67741 cgtgcgacaa atgacgtcga tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg
    67801 gtggattcac tggtaatggg ctgcgctgtg ttgattatga tgtctacgca aattagctgg
    67861 cagttgacct tatttgccct gttgccgatg ccagtgatgg cgatcatgat caagcgcaac
    67921 ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg cgttttccag tcttaatgac
    67981 cgcacccagg aaagcctcac cagtatccgc atgatcaaag cctttggtct ggaagatcgt
    68041 cagtcggcgt tatttgccgc ggatgccgaa gataccggca aaaaaaacat gcgggtggcg
    68101 cgtattgatg ctcgtttcga cccgaccatc tatatcgcga ttggtatggc gaacttgctg
    68161 gcgatcggcg gcggtagttg gatggtggtg cagggcagtt taacgctggg ccagctcacc
    68221 agttttatga tgtatttagg tctgatgatt tggccaatgc tggcgctggc atggatgttt
    68281 aacattgtgg aacgtggcag cgcggcgtat agccgaattc gtaccatgct ggccgaagcg
    68341 ccagtcgtta ttgatggtag tgataaggtg ccagaaggtc gtggtgaact ggatgtgaat
    68401 attcgccagt ttacgtatcc gcagactgac catcctgcgc tggaaaacgt caatttcgcc
    68461 ctgaaacccg gtcagatgct gggtatctgc gggccgactg gttccggcaa aagtaccctg
    68521 ttgtcgctca ttcagcgtca tttcgacgtc agcgagggtg atatccgttt tcatgatatt
    68581 cctctgacga agttacaact cgatagctgg cgtagccgtc tggcggtggt cagccagaca
    68641 ccattccttt tttccgacac cgtggcgaac aacatcgcgt tgggttgccc gaatgccacc
    68701 cagcaagaga ttgaacacgt agcgcggtta gccagcgtac atgacgatat tttgcgtctg
    68761 ccgcaaggtt acgacacaga agtgggcgag cgcggtgtga tgctttctgg cggacaaaaa
    68821 cagcgtatct ccattgctcg agcgttatta gtcaacgcgg aaatcctcat ccttgatgat
    68881 gcgctttcag cggtggacgg acgcacagag caccagatcc tgcataacct gcgtcagtgg
    68941 gggcagggaa gaacggtaat catcagtgcc catcgccttt ctgcactgac ggaagccagt
    69001 gaaattattg tgatgcagca cgggcatatc gcccagcgtg gcaatcatga tgtgctggta
    69061 caacaaagcg gctggtatcg cgatatgtat cgctatcaac aactggaggc ggcgctcgac
    69121 gacgccccgg aaattcgcga ggaggccgtc gatgcgtagt tttagccaac tgtggccgac
    69181 tctcaagcgt ctgttagcgt acggttcacc gtggcgtaaa ccgttgggga ttgcggtcct
    69241 gatgatgtgg gttgcggcgg cggcagaagt cagtgggccg ctgcttatca gctattttat
    69301 cgacaatatg gtagcgaaaa ataacctgcc gttgaaagtg gttgcagggc tggcggcggc
    69361 gtatgttggg ctgcaactgt ttgcagccgg gctacattac gcgcagtcgc tgctgtttaa
    69421 tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg
    69481 ccagccatta agcgagtttg atacccaacc cgtcgggcag gtgatttccc gcgtcactaa
    69541 tgacactgaa gtgatccgcg atctctacgt taccgtagtg gcaactgtcc tgcgtagcgc
    69601 cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc gactggcgaa tggcgctggt
    69661 ggcgataatg attttcccgg tggtgctggt ggtaatggtg atatatcagc gttacagcac
    69721 gccgattgtc cgtcgtgtgc gcgcttatct ggcggatatc aacgacggct ttaacgaaat
    69781 catcaatggc atgagcgtta tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat
    69841 gggtgaggcc agtcgttcac actatatggc gaggatgcaa accctgcgcc tcgacggttt
    69901 tctgctgcgt ccgctgctga gtctgttttc atcgctcatt ctttgtggct tgttgatgct
    69961 gtttggcttc tccgccagcg gcaccattga agtgggcgtg ttgtatgcgt tcatcagtta
    70021 tttggggcga cttaacgaac ccttaatcga actgaccacg cagcaggcga tgctgcaaca
    70081 ggctgttgtt gctggtgagc gcgtttttga gctgatggac ggaccgcgcc agcaatatgg
    70141 caatgatgat cgctcgttgc agagcggcac catcgacgtc gataacgtgt catttgctta
    70201 tcgcgatgac aatctggtgc taaagaacat taatctctct gtgccttcgc gcaactttgt
    70261 ggcgctggtc gggcataccg gcagtggcaa aagcaccctc gccagtttat tgatgggcta
    70321 ttacccgcta acggaaggtg agattcgcct tgatggtcgt ccattaagta cgctaagtca
    70381 cagcgcgctg cgccagggcg tggcaatggt gcagcaagat ccggtggtgc tggcggatac
    70441 cttcctcgcc aacgtgacgc tgggacgtga tatctctgaa gaacgcgtct ggcaggcgct
    70501 ggaaaccgtg cagctggcgg agctggcgcg tagcatgagt gatggtattt acacgctgct
    70561 tggcgagcag gggaataatc tctcagtcgg gcaaaagcaa ctgctggcac tggcgcgcgt
    70621 gctggtggag acgccgcaaa tcctgatcct tgatgaggca accgccagca ttgactccgg
    70681 gactgaacag gcgattcaac atgctctggc ggcggtgcgt gaacatacta cgcttgtggt
    70741 gattgctcac cgcttatcga ctattgttga tgccgacacc attctggtgc ttcatcgtgg
    70801 gcaagccgtg gagcagggca ctcaccagca actgctggcg gcccagggac gatactggca
    70861 gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc agcgtgcgtg aagaggaatc
    70921 attgagcgcc tgaatagcgc aatatttcat cgttggtgca aaaatgtaac gcactgtgca
    70981 ctgtcatagt gcgttttcat tttcaatctt cttaacttcc tgctctcttt ctcgtttttc
    71041 atttctggca caccgcttgc aataccttct tcgtgtagca gaaccattac cgaattctga
    71101 ccggagggga tctatgaagc tggtgaccgt gataatcaaa ccattcaagc tggaagacgt
    71161 tcgtgaagcg ttatcttcca ttggtattca gggcctgacc gtcaccgaag tgaaaggttt
    71221 tgggcgtcag aaagggcatg ccgagctgta ccggggggcg gaatacagcg tcaacttcct
    71281 gccaaaagta aaaattgatg tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat
    71341 cgtcagcaaa gcggcttaca ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt
    71401 gcaacgcgtc attcgtattc gtaccggcga agccgacgaa gcggcgctgt aatctctggc
    71461 acacagcaac aggaacgaaa aatgaagata gcaacgataa aaactgggct tacttcactg
    71521 gcgatgctgc cgggactggt aatggctgca cctgcggtgg ccgataaagc cgacaatgca
    71581 tttatgatga tttgtactgc gctggtgctg tttatgacta ttccggggat tgccctgttt
    71641 tatggagggc tgattcgtgg caaaaacgtg ctgtcgatgc tgacgcaggt gaccgtgacg
    71701 tttgcactgg tctgtattct ctgggtggtt tacggctact cgctggcgtt tggcgaaggc
    71761 aacaacttct tcggcaacat taacgggttg atgctgaaaa acattgaact gacggcggtg
    71821 atgggcagca tttatcagta tatccacgtg gcgtttcagg gatcgtttgc ctgcattacc
    71881 gtaggcttga tagttggggc gctggcggaa cgaatccgct tctcagctgt gttgattttt
    71941 gtggtggtat ggctgacgct ctcttacatt ccgattgcgc atatggtctg gggcggcggt
    72001 ttgctggctt ctcacggtgc gctggatttc gcgggtggca cggtagtgca cattaacgcc
    72061 gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac gcgtgggctt cggcaaagag
    72121 gcgtttaaac cgcacaacct gccgatggtc ttcaccggga ctgccattct ctatatcggt
    72181 tggtttggct ttaacgccgg gtcagcgggc acggcgaatg aaatcgcggc actggcattt
    72241 gtgaatactg tggtcgcaac cgcggcggca attcttggct ggatcttcgg tgaatgggcg
    72301 ctgcgtggta agccttcact gctgggagcg tgttctggcg cgattgccgg tctggtcggc
    72361 gtgacgccag cctgtggtta cattggggtt ggcggcgcgt tgattatcgg cgtggtagct
    72421 ggtctggcgg gcttgtgggg cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc
    72481 tgcgatgtct tcggtgtgca cggcgtttgt ggtattgtcg gctgtatcat gaccgggatt
    72541 tttgccgcca gctcgctggg cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag
    72601 ttgctggtac agctggaaag catcgccatt acgattgtct ggtctggtgt ggtggcattt
    72661 atcggctaca aactggcgga tctgacggtt ggtctgcgtg taccggaaga gcaggagcga
    72721 gaagggctgg acgtcaacag ccacggcgag aatgcctata acgcgtaaca agcactgcaa
    72781 aaaacagccg gacggttttc acctccggct atttttttaa ttgtgattac gcatcacccc
    72841 ttcctgaacg gtcgaggcaa ccagcacgcc gtcctgggta taaaactcac cgcgcacaaa
    72901 gccgcgtgcg ctggacgccg aggtgctttc cacgctatac agcagccatt cattcaaatt
    72961 aaacgggcga tggaaccaca tggaatggtc aatggtggca atctgaatcc ccggttcgag
    73021 aaaaccgatg ccgtgcggct gtagagctac cggcaggaaa ttaagatcag aagcataacc
    73081 gagcagatac tgatgaacgc gcagatcatc cggcacgcta ccatttgcgc ggatccacac
    73141 ctgacgatgc ggttctgcga cgtgaccttt cagtgggtta tgaaactcca ccggacggac
    73201 ttccagcgga cgatcgcaga tgaatttatc tttcagcact ggcggcagca ggtgcgccag
    73261 cgattgggcg atttgcgttt ctgaaggcag gccatcaggc gctggtgcgg acggcattgt
    73321 tttttgatgt tcgaaacccg cttctggtgc ctggaaagag gcagtcatat aaaaaatcgg
    73381 tttgccgttt tgaatagcag caacccggcg ggcgctgaag ctgttaccgt cacgcagtgt
    73441 ttcgacatca taaataatcg gcttcttact atcgccaggg cgaagaaagt agctgtgaaa
    73501 agaatgtacc agccgctcgt cagggacggt ctcttttgca gcatacaagg cctgacccac
    73561 gacctggccg ccaaatacct ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc
    73621 ttcctcaatt ttttccagat ttaacaatgt cagtaaattt tttagcgcct gactcatata
    73681 actctccagt aacaaagctg ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg
    73741 ccactggtcc gatgggtgca atggtctgaa ttacgggcta attacaggca gaaatgcgtg
    73801 atgtgtgcca cacttgttga cgttactatt ttgttaacca ctcttccggc gaggaaagtt
    73861 agcccgctgg tgcattgata ataaggagaa gtgaatgaaa ctcgtgcaca tggccagtgg
    73921 tttagcggtt gcgattgcgt tggcggcttg cgcagataaa agcgcggata ttcagacgcc
    73981 agcaccggct gcaaatacgt ctatttcagc aacacaacaa cctgctatcc agcaaccgaa
    74041 tgtctccggt accgtttgga tccgtcagaa agtcgcactg ccgcctgatg ccgtgctgac
    74101 cgtgacactt tctgacgcgt cgttagccga tgcgccgtcg aaagtgctgg cgcagaaagc
    74161 ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt ctgccattta acccggcaga
    74221 tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt accgtgaatg acaaactggt
    74281 atttattacc gataccgttc agccggtgat caaccagggc ggaactaaag ccgacctgac
    74341 attggtgccg gtgcagcaaa ccgccgtgcc ggttcaggct agcggtggcg caacgactac
    74401 cgtaccttca acttcaccaa cccaggtgaa tccgtcttcg gcagttccag ctccaacgca
    74461 atattaagcg aagttaaccc tctccgcccg gagagggtta gtagttccag cgataaagct
    74521 gcaagtcgat ttgcccgctt cccgacacca tcacaccttc tgccagtaat gcctgtcgct
    74581 gacgctgtaa atccggtccg gtcagtgaaa tggtgccgtg gcgattaacc acccggtgcc
    74641 agggcaaggt gctgccttcg gggagacgct ttaacacacc gccaacctgg cgcgcggcac
    74701 ggggcgagcc cgccagtttc gccacatcac cgtaagtggt gacatagcct tcgggaatag
    74761 cggctacgat ttgccagacg cgttggggaa atgaatcttc tttttccatc ttttcttcct
    74821 gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg aagccgcatt gcgcaagaaa
    74881 ccagcatctg gcacgcaatg ggttgcaatt agccggggca gcagtgataa tgcgcctgcg
    74941 cgttggttct caacgctctc aatgggggct ctgttggttc tcccgcaacg ctactctgtt
    75001 taccaggtca ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc
    75061 tggcagggcc cccacccatt tctgcctcct accatttcgt caaaaaatcc caacatggct
    75121 aaactttaac cacaactgac gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt
    75181 atgtctgatg aagtatgttg atggttttgt tgttgccgtt cctgccgata aaaaggatgc
    75241 ctatcgggaa atggccgcta aggccgcgcc attatttaaa gagttcgggg cgcttcgtat
    75301 tgtcgaatgc tgggccagcg atataccgga tggtaaagtg accgattttc gtatggcggt
    75361 gaaagcggaa gagaatgaag aggttgtctt tagctggatt gaataccctt caaaagaggt
    75421 ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg atgaaagagt tcggcgagtc
    75481 catgccgttt gatggtaagc gaatgatcta tggcggattc gagtcaatca tcgacgagta
    75541 ggtagcgtga cgggctgtgc ggcgtgccgt cagccctata attcacaaat gttgctcggc
    75601 ccagcgtaag aaatcctctt tcggtaatgc cttgctgtag agccagccct gaccgtagtg
    75661 cacgccatgc tggcgtagcc actcttcttg tttactggtt tcgattccct ccgcaaccat
    75721 tttcagtttc agtgtttttg ccatttcgat gatatgtggc gtgacattct tatattccag
    75781 cgcatcaacg aaagatttat cgatcttcag aatgtcgaca tccagatcct gtaaatagct
    75841 taaacttgaa taccctgtac caaaatcatc gagataaatt tcatggcccg cctcccggta
    75901 gcgagaaatt atcggggcgc tggttttcgg atcggcaaac tcgcgttcag tgagttcaag
    75961 cgcgatctgt ctgggattaa cctgatagtg attgatcatt tcacgcagca attgcgggat
    76021 tttttccgag gtgagcacgg tggattcaag attgatcgaa atatgctgct gaggatgctg
    76081 acgcagccag tcgcccatat cttcaaagac gcttcttata atcagtagcg tcaatggctc
    76141 agaaaggccc gtttgctgtg ccagcggaat aaaactatct ggtgacaacc aactaccgtc
    76201 tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt ttgccattgg ctaaggagac
    76261 aatcggctga tagtgcacgc aaatatcacg attttcgata gcatcctgca gccgatgatg
    76321 cggtgactgg atacggcgca aaatacgcag cacaaacatc gctgccagca ggccaatcac
    76381 caacccgagc ggtaaccaaa taaagacttg ccgataccag cctttctgca acattttcgt
    76441 tgaagcccag gtgatgatcg aaatattcat ctccggtaag ggcaggatat cgtaaatgat
    76501 tccattattt tcgatatgct caccgggtgt tttttgtagc ctggtaataa ttccctgagc
    76561 aagttcatcg ctgctggtta tgacaacgtt atgggcattg ccaataatgg cggcatcaat
    76621 ttgccatgag ctatagggaa tgacatcaat aaaggaagcg gggtcgatca tgacgacata
    76681 atgtgccgtt cccatggcga ccatgtaacg gataatgcct aaatcgttat gcgatgttaa
    76741 ccagacacga taaccatctt tcgaaatttt acctggctcg gggaaggtat cgggcgggct
    76801 ttcatgctcc agagacgaac attggggaac gttgttatcg atataaacca cttcctgaat
    76861 atagcgataa ctgtaagata cccgacgcat ttccatgaga tgggcttcgc tacaggcagc
    76921 gccttgccat ctttccagct cttgcagcgc atctttccct tgtgtcgcca ccttattggc
    76981 ccgaatagcg acgcgggagg aatacgtatt cagctcttca ataaatgacg tttctacctg
    77041 ctgatgggcc agccagatgc ttaagccgac aggcagcaat actgaaagaa tcagtactcc
    77101 cgaaataagg ccgaccagat gtcgtgttct caccagcata tccttattaa cacagtgtag
    77161 ggaaagtata tctgattgtg gaaaaaggct ggcaggagat aaggaggtag agctggtatt
    77221 tcatgaagtg atgggtggaa gcaatcgttg tcatttgacc aggcgtagag ctttacgcct
    77281 ggtatgtacc cctaacttaa gatgttgatt cggctcgagc gagggtaaaa atatcgtaaa
    77341 aagatagttc acctttacgg acgatcattt tttgcagttg ttctttatga acatccgcga
    77401 cacgtggaga gttcaggatg gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc
    77461 attcgccgtt atagcaaacg cggaaatcca gcacgtcgat atcttcatag cggtagcgtg
    77521 ggtagacatc gaaaaagaag aaaccattaa gcaggataaa taatacaacc agtagccaga
    77581 cagagcctga aagcgtttcg gattgcaaca tcaccgccag ggtagcaaaa aaagcaacgt
    77641 acataccgat aaacagcccc ggatgtttac ggataaaact gatactaaag cggggtttgt
    77701 tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc ggtcagcagg cgttgtattt
    77761 cggtcatcgt ttgccttcgt gatatctgca actttccagg gaacacccta ttttaacgtg
    77821 ggaatagtag aggggaagta acagatttgc cacgattttg cataacagtt gagaaaagct
    77881 ggttacaatt ttttaatgat tctggctgga ttaccgccca cgacaacgtt gtccgggaca
    77941 tcttttgtga caactgcacc tgaggctacc acgacgttat caccaatggt cacaccaggg
    78001 ttaatgactg cacgtccgcc aatccagacg ttattaccga tggtgacggg tttccccagt
    78061 tcagcaccgc tattacgtgc tacagggtcg atgggatgtg ttgccgtgta gatatgaacg
    78121 cctggtgcca acatacagtt atcaccaatg cgaatagggc agacatcaag catcacgcaa
    78181 tcgaagttgg cgaaaaaatt attgccgaga aaaatgttat agccatagtc acagcgaaac
    78241 gttggctcaa tataagcctc tgtcacctga ccgaataaat cagcaagaat ttgctggcgt
    78301 aatgtgtgct cttccgccag ggaatgattg tatcggtgaa taagccgacg agcgcgcagg
    78361 cgatcgcgag ataacgcctc atctgccgag tgatacaact caccagcaat catcttttct
    78421 ttttctgtgc tcattactga cttccggttg ctgaaaatgc ggcaacacag taagacgaaa
    78481 agtcagtgaa gagaacgttc ccattacatc tgtgtgatga agatcacata gggtatgcct
    78541 tcatggcgaa aaaaagtata aaattcttaa taaacagccg gttatagctc cgaaagcgag
    78601 ttatttatta gcgaataaat ttccatactg aggaagggat cttgtcgtac agtttattca
    78661 tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa taccgccagt tcattatctg
    78721 ataattcgta tttatttttc tcgataacac gctccagcgt gtcaattgtc tggcaacgac
    78781 gtaaacgcat taaataatcg gttttcgtta aaggtttttc ggacatactt ctacccatgg
    78841 ttgtaataat tctaacaaga taaactcgca ggattctctt tcgtcgcatt gacaaaacaa
    78901 cggaatagtc gattacctga cttccgccat ttctgaagat cctgcatatt aataccataa
    78961 ctactgaaca acataaaggt gtcatccaga tattcgtcga tctgctcaat gagcttattg
    79021 tcttcattat acttaatttt gtaattaagt gcgaaggtcg caatatgctc aatcagttca
    79081 ttcaactgga ggttgatcgc cgaggttggg tcgttaaccc agccatgatt gctttcttca
    79141 aggtttgcaa ggcagtcatg atacagggtt tcacagagaa acttaagctg tgcgatatca
    79201 tgtcttttgg gtgagtattc atccataacg cgtccccttc ttagcggttg aactaacgga
    79261 cacctttcgg gatggaaaaa acttactgac ctggacttgc cctctcgttt gttagcttaa
    79321 ctataagcca ctctttgcag gttttcatcg cattattacg aaaaattaca attaggaagg
    79381 aagcgggaca ggactcactt tttcatacta gcaccaaccc gcagcaggta aaagcagttt
    79441 gctcgaactg ttcataacat ttcagaatta tcgccagaag aaattggtga cactaatacc
    79501 aggattgctc tgaatatgac gtaataaccg aggaatgaat aaagaattac cgcaaataa
//
LOCUS       DAJOTB010000006        71814 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJOTB010000006 DAJOTB010000000
VERSION     DAJOTB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.006"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            214..1614
                     /gene="sthA"
                     /locus_tag="OHN28_000415"
     CDS             214..1614
                     /gene="sthA"
                     /locus_tag="OHN28_000415"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCQ2295861.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(1597..2514)
                     /gene="oxyR"
                     /locus_tag="OHN28_000416"
     CDS             complement(1597..2514)
                     /gene="oxyR"
                     /locus_tag="OHN28_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCQ2295862.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2781..4154)
                     /gene="argH"
                     /locus_tag="OHN28_000417"
     CDS             complement(2781..4154)
                     /gene="argH"
                     /locus_tag="OHN28_000417"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCQ2295863.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(4215..4991)
                     /gene="argB"
                     /locus_tag="OHN28_000418"
     CDS             complement(4215..4991)
                     /gene="argB"
                     /locus_tag="OHN28_000418"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCQ2295864.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(4999..6003)
                     /gene="argC"
                     /locus_tag="OHN28_000419"
     CDS             complement(4999..6003)
                     /gene="argC"
                     /locus_tag="OHN28_000419"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCQ2295865.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            6157..7308
                     /gene="argE"
                     /locus_tag="OHN28_000420"
     CDS             6157..7308
                     /gene="argE"
                     /locus_tag="OHN28_000420"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCQ2295866.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            7610..10261
                     /gene="ppc"
                     /locus_tag="OHN28_000421"
     CDS             7610..10261
                     /gene="ppc"
                     /locus_tag="OHN28_000421"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCQ2295867.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            10444..12177
                     /locus_tag="OHN28_000422"
     CDS             10444..12177
                     /locus_tag="OHN28_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCQ2295868.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            12403..13254
                     /locus_tag="OHN28_000423"
     CDS             12403..13254
                     /locus_tag="OHN28_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2295869.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(13241..13582)
                     /locus_tag="OHN28_000424"
     CDS             complement(13241..13582)
                     /locus_tag="OHN28_000424"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2295870.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13584..14462)
                     /locus_tag="OHN28_000425"
     CDS             complement(13584..14462)
                     /locus_tag="OHN28_000425"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCQ2295871.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(14428..16725)
                     /locus_tag="OHN28_000426"
     CDS             complement(14428..16725)
                     /locus_tag="OHN28_000426"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2295872.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(16776..17096)
                     /locus_tag="OHN28_000427"
     CDS             complement(16776..17096)
                     /locus_tag="OHN28_000427"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2295873.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(17111..18190)
                     /locus_tag="OHN28_000428"
     CDS             complement(17111..18190)
                     /locus_tag="OHN28_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCQ2295874.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            18499..21000
                     /gene="ptsP"
                     /locus_tag="OHN28_000429"
     CDS             18499..21000
                     /gene="ptsP"
                     /locus_tag="OHN28_000429"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2295875.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            21012..21674
                     /gene="fsa"
                     /locus_tag="OHN28_000430"
     CDS             21012..21674
                     /gene="fsa"
                     /locus_tag="OHN28_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2295876.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            21685..22788
                     /gene="gldA"
                     /locus_tag="OHN28_000431"
     CDS             21685..22788
                     /gene="gldA"
                     /locus_tag="OHN28_000431"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCQ2295877.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            23064..23681
                     /locus_tag="OHN28_000432"
     CDS             23064..23681
                     /locus_tag="OHN28_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCQ2295878.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(23708..24613)
                     /gene="yijE"
                     /locus_tag="OHN28_000433"
     CDS             complement(23708..24613)
                     /gene="yijE"
                     /locus_tag="OHN28_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCQ2295879.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(24707..26887)
                     /gene="katG"
                     /locus_tag="OHN28_000434"
     CDS             complement(24707..26887)
                     /gene="katG"
                     /locus_tag="OHN28_000434"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCQ2295880.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(27216..28106)
                     /gene="metF"
                     /locus_tag="OHN28_000435"
     CDS             complement(27216..28106)
                     /gene="metF"
                     /locus_tag="OHN28_000435"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCQ2295881.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(28488..30041)
                     /locus_tag="OHN28_000436"
     CDS             complement(28488..30041)
                     /locus_tag="OHN28_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCQ2295882.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            30174..31304
                     /locus_tag="OHN28_000437"
     CDS             30174..31304
                     /locus_tag="OHN28_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCQ2295883.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(31268..33700)
                     /gene="metL"
                     /locus_tag="OHN28_000438"
     CDS             complement(31268..33700)
                     /gene="metL"
                     /locus_tag="OHN28_000438"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCQ2295884.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(33703..34863)
                     /gene="metB"
                     /locus_tag="OHN28_000439"
     CDS             complement(33703..34863)
                     /gene="metB"
                     /locus_tag="OHN28_000439"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCQ2295885.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            35140..35457
                     /gene="metJ"
                     /locus_tag="OHN28_000440"
     CDS             35140..35457
                     /gene="metJ"
                     /locus_tag="OHN28_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCQ2295886.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            35517..36125
                     /locus_tag="OHN28_000441"
     CDS             35517..36125
                     /locus_tag="OHN28_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCQ2295887.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(36186..36398)
                     /gene="rpmE"
                     /locus_tag="OHN28_000442"
     CDS             complement(36186..36398)
                     /gene="rpmE"
                     /locus_tag="OHN28_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCQ2295888.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            36601..38799
                     /gene="priA"
                     /locus_tag="OHN28_000443"
     CDS             36601..38799
                     /gene="priA"
                     /locus_tag="OHN28_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCQ2295889.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            38955..39980
                     /gene="cytR"
                     /locus_tag="OHN28_000444"
     CDS             38955..39980
                     /gene="cytR"
                     /locus_tag="OHN28_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCQ2295890.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            40072..41031
                     /gene="ftsN"
                     /locus_tag="OHN28_000445"
     CDS             40072..41031
                     /gene="ftsN"
                     /locus_tag="OHN28_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCQ2295891.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            41124..41654
                     /gene="hslV"
                     /locus_tag="OHN28_000446"
     CDS             41124..41654
                     /gene="hslV"
                     /locus_tag="OHN28_000446"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCQ2295892.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            41664..42995
                     /gene="hslU"
                     /locus_tag="OHN28_000447"
     CDS             41664..42995
                     /gene="hslU"
                     /locus_tag="OHN28_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCQ2295893.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            43062..43988
                     /gene="menA"
                     /locus_tag="OHN28_000448"
     CDS             43062..43988
                     /gene="menA"
                     /locus_tag="OHN28_000448"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCQ2295894.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            44081..44566
                     /gene="rraA"
                     /locus_tag="OHN28_000449"
     CDS             44081..44566
                     /gene="rraA"
                     /locus_tag="OHN28_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCQ2295895.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(44651..44896)
                     /gene="zapB"
                     /locus_tag="OHN28_000450"
     CDS             complement(44651..44896)
                     /gene="zapB"
                     /locus_tag="OHN28_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCQ2295896.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            45321..46166
                     /gene="glpF"
                     /locus_tag="OHN28_000451"
     CDS             45321..46166
                     /gene="glpF"
                     /locus_tag="OHN28_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCQ2295897.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            46189..47697
                     /gene="glpK"
                     /locus_tag="OHN28_000452"
     CDS             46189..47697
                     /gene="glpK"
                     /locus_tag="OHN28_000452"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCQ2295898.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            47868..48878
                     /gene="glpX"
                     /locus_tag="OHN28_000453"
     CDS             47868..48878
                     /gene="glpX"
                     /locus_tag="OHN28_000453"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2295899.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            48975..49721
                     /gene="fpr"
                     /locus_tag="OHN28_000454"
     CDS             48975..49721
                     /gene="fpr"
                     /locus_tag="OHN28_000454"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCQ2295900.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(49726..50154)
                     /gene="uspD"
                     /locus_tag="OHN28_000455"
     CDS             complement(49726..50154)
                     /gene="uspD"
                     /locus_tag="OHN28_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCQ2295901.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(50181..50480)
                     /locus_tag="OHN28_000456"
     CDS             complement(50181..50480)
                     /locus_tag="OHN28_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCQ2295902.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(50692..51132)
                     /locus_tag="OHN28_000457"
     CDS             complement(50692..51132)
                     /locus_tag="OHN28_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2295903.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            51233..51832
                     /locus_tag="OHN28_000458"
     CDS             51233..51832
                     /locus_tag="OHN28_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCQ2295904.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            51940..52707
                     /gene="tpiA"
                     /locus_tag="OHN28_000459"
     CDS             51940..52707
                     /gene="tpiA"
                     /locus_tag="OHN28_000459"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCQ2295905.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(52762..53517)
                     /gene="cdh"
                     /locus_tag="OHN28_000460"
     CDS             complement(52762..53517)
                     /gene="cdh"
                     /locus_tag="OHN28_000460"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCQ2295906.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(53624..54613)
                     /gene="sbp"
                     /locus_tag="OHN28_000461"
     CDS             complement(53624..54613)
                     /gene="sbp"
                     /locus_tag="OHN28_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCQ2295907.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(54933..55895)
                     /gene="pfkA"
                     /locus_tag="OHN28_000462"
     CDS             complement(54933..55895)
                     /gene="pfkA"
                     /locus_tag="OHN28_000462"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCQ2295908.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(56076..56978)
                     /gene="fieF"
                     /locus_tag="OHN28_000463"
     CDS             complement(56076..56978)
                     /gene="fieF"
                     /locus_tag="OHN28_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCQ2295909.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(57127..57627)
                     /gene="cpxP"
                     /locus_tag="OHN28_000464"
     CDS             complement(57127..57627)
                     /gene="cpxP"
                     /locus_tag="OHN28_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCQ2295910.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            57777..58475
                     /gene="cpxR"
                     /locus_tag="OHN28_000465"
     CDS             57777..58475
                     /gene="cpxR"
                     /locus_tag="OHN28_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCQ2295911.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            58472..59845
                     /gene="cpxA"
                     /locus_tag="OHN28_000466"
     CDS             58472..59845
                     /gene="cpxA"
                     /locus_tag="OHN28_000466"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCQ2295912.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(60016..60690)
                     /gene="yiiM"
                     /locus_tag="OHN28_000467"
     CDS             complement(60016..60690)
                     /gene="yiiM"
                     /locus_tag="OHN28_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCQ2295913.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSVAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(60839..61822)
                     /gene="kdgT"
                     /locus_tag="OHN28_000468"
     CDS             complement(60839..61822)
                     /gene="kdgT"
                     /locus_tag="OHN28_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCQ2295914.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(62082..62702)
                     /gene="sodA"
                     /locus_tag="OHN28_000469"
     CDS             complement(62082..62702)
                     /gene="sodA"
                     /locus_tag="OHN28_000469"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCQ2295915.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQAYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            62987..64021
                     /gene="rhaT"
                     /locus_tag="OHN28_000470"
     CDS             62987..64021
                     /gene="rhaT"
                     /locus_tag="OHN28_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCQ2295916.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(64018..64884)
                     /gene="rhaR"
                     /locus_tag="OHN28_000471"
     CDS             complement(64018..64884)
                     /gene="rhaR"
                     /locus_tag="OHN28_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCQ2295917.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(64940..65776)
                     /gene="rhaS"
                     /locus_tag="OHN28_000472"
     CDS             complement(64940..65776)
                     /gene="rhaS"
                     /locus_tag="OHN28_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCQ2295918.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHCVLQQVRQLVAQLEQQEEGNDLPSTASREILFMQLLLLLRKS
                     SLQESVENSASRLNLLLAWLEDHFADEVNWDAVAAQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            66064..67533
                     /gene="rhaB"
                     /locus_tag="OHN28_000473"
     CDS             66064..67533
                     /gene="rhaB"
                     /locus_tag="OHN28_000473"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCQ2295919.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            67530..68789
                     /gene="rhaA"
                     /locus_tag="OHN28_000474"
     CDS             67530..68789
                     /gene="rhaA"
                     /locus_tag="OHN28_000474"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCQ2295920.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLLDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            69056..69880
                     /gene="rhaD"
                     /locus_tag="OHN28_000475"
     CDS             69056..69880
                     /gene="rhaD"
                     /locus_tag="OHN28_000475"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCQ2295921.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            69890..70204
                     /gene="rhaM"
                     /locus_tag="OHN28_000476"
     CDS             69890..70204
                     /gene="rhaM"
                     /locus_tag="OHN28_000476"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCQ2295922.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(70245..71639)
                     /locus_tag="OHN28_000477"
     CDS             complement(70245..71639)
                     /locus_tag="OHN28_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2295923.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        17524 a        19216 c        18273 g        16801 t
ORIGIN      
        1 taaagacaat aacaaaaaag cacgcttcgg caagataaaa ctatcagcca taagaaaaag
       61 cgattaatag tgaataaata aaataagaag gaggaagaca aagcaaaggc cgctcacgat
      121 atagccagat aaatgacggg gatcaattgg cttacccgcg ataaaatgtt accattctgt
      181 tgcttttatg tataagaaca ggtaagccct accatgccac attcctacga ttacgatgcc
      241 atagtaatag gttccggccc cggcggcgaa ggcgctgcga tgggcctggt taagcaaggt
      301 gcgcgcgtcg cagttatcga gcgttatcaa aatgttggcg gcggttgcac ccactggggc
      361 accatcccgt cgaaagctct ccgtcacgcc gtcagccgca ttatagaatt caatcaaaac
      421 ccactataca gcgaccattc ccgactgctc cgctcttctt ttgccgatat ccttaaccat
      481 gccgataacg tgattaatca acaaacgcgc atgcgtcagg gattttacga acgtaatcac
      541 tgtgaaatat tgcaggggaa cgctcgcttt gttgatgagc atacgttggc gctggattgc
      601 ccggacggca gcgtcgaaac actaactgct gaaaaatttg ttattgcctg cggttctcgt
      661 ccatatcatc caacagatgt tgatttcacc catccacgca tttatgacag cgactcaatt
      721 cttagcatgc accacgaacc gcgccatgta cttatttacg gtgctggagt gatcggctgt
      781 gaatatgcgt cgatcttccg cggtatggat gtaaaagtgg atctgatcaa cacccgcgat
      841 cgcctgctgg catttctcga tcaagagatg tcagattctc tctcctatca cttctggaac
      901 agtggcgtag tgattcgtca caacgaagag tacgagaaga tcgaaggctg tgatgacggt
      961 gtgatcatgc atctgaagtc gggtaaaaaa ctgaaagctg actgtctgct ctatgccaac
     1021 ggtcgcaccg gtaataccga ttcgctggcg ttacagaaca ttgggctgga aactgacagt
     1081 cgcggacagc tgaaggtcaa cagcatgtat cagaccgcac agccgcacgt ttacgcggtg
     1141 ggcgacgtga ttggttatcc gagcctggcg tcggcagcct atgaccaggg gcgcattgcc
     1201 gcgcaggcgc tggtgaaagg tgaagccacc gcacatctga ttgaagatat ccctaccggc
     1261 atttacacca tcccggaaat cagctctgta ggcaaaaccg aacagcagct gaccgcgatg
     1321 aaagtgccat atgaagtagg ccgcgcccag tttaaacatc tggcacgtgc acaaatcgtc
     1381 ggcatgaacg tgggcacgct gaaaattttg ttccatcggg aaacaaaaga gattctgggt
     1441 attcactgct ttggcgagcg cgctgccgaa attattcata tcggtcaggc gattatggaa
     1501 cagaaaggtg gcggcaacac tattgagtac ttcgtcaaca ccacctttaa ctacccgacg
     1561 atggcggaag cctatcgggt agctgcgcta aatggcttaa accgcctgtt ttaacacttt
     1621 atcgaaatgg ccatccattc ttgcgcggat ggcctctgcc agctgctcat agcggctgcg
     1681 cagcggtgag ccaggacgat aaaccaggcc aatagtgcgg cgtggttccg gcttaatgca
     1741 cggcagataa acaaccccat cgcgtttgcg ttccggcggc acagccagcg caggcagtaa
     1801 agtgatcccg ctacctgccg ccaccatgtt gcgcagagtt tccaggctgg tcgcgcggaa
     1861 gtgtgtatct tcatccgccc cggcttcaaa acagaaaccc attgcctgat cgcgcaaaca
     1921 gtgaccatct tccagcatca gcagtttttc ccctgccaga tcggccatcg gtacgcattc
     1981 gcggttcgcc cacgggtggt cttcatagat agccagcaac attggctcat caaacaacgg
     2041 cacttcaatg aatgcttcgc tctctttcac cagcgcgagg atcacgcaat cgagtttgcc
     2101 gctgtccagt tgcgccagta actggtgggt ctgtgcttca tgcagataca tttccagctt
     2161 tggaaaggtc tggtgcagca tcgggataat atgcggtagc aggtacggtc caaccgtggg
     2221 aatcaaacca atatgcagcg gtccggacat cgtctcgccc tgctggcttg ccatctcttt
     2281 aaggactttc acctcacgca gcacggtacg cgcctgatcc accagcagca ttcccgcctg
     2341 ggtgaataac actttacggc tggtccgctc cagcaacatc acgcccagct catcttccag
     2401 cttacgaatt tgcccgctaa gcgtcggctg gctaacgtgg caggaatctg ccgcacgccg
     2461 aaaatggcgg tgttcagcca atgccaccag gtactcaaga tcacgaatat tcattatcca
     2521 tcctccatcg ccacgatagt tcatggcgat aggtagaata gcaatgaacg attatcccta
     2581 tcaagcattc tgactgataa ttgctcacag aaacggagcg gcacctcttt taacccttga
     2641 agtcacagcc cgtttcgaga gtttctcagc tcgaataact aaagccaacg tgaacttttg
     2701 cggatctcca ggatccgctt ttttttgcca taaaaaagcc cagcgataag ccaggctcaa
     2761 atttatacat ataaatgttc ttaccccaac cgcgcctgcg caaaagcaat cgcctgcgcc
     2821 acctgttgcg gtgagacacc gccttttgcc gcacgcttgt cgaggcacga ttgcagcgac
     2881 agaatcggat agacatcttc gccaatcacc aggctgaatt tctgcaactc actgagtggc
     2941 agatcttcca gcggtttgcc ctgacgaatg gcttccacca ccgcttcacc cacaatatgg
     3001 tgcgcctcgc ggaacggtac gcctttcgcc accagataat ccgccagttc agtagcgttg
     3061 gagtaaccct gctgcgccgc ttcctggcaa cgcggacgtt tcacctgaat gccgtccagc
     3121 accagcgccg ccatatgcag gcagtccagc caggtatcga gcgcgtcgaa cagaccttct
     3181 ttgtcttcct gcatatcttt gttgtaagcc agcggcaaac ctttcagcgt catcatcatg
     3241 ccggttaacg ccccctgcac ccgaccgcat ttaccgcgaa tcagctccag cgcatccggg
     3301 tttttcttct gcggcattaa tgatgaaccg gaagtcacac ggtcagaaag ctccacaaac
     3361 cccgcttcgc cggtgttaaa gaaaatcaga tcttcagcaa aacgcgacag atgcaccatg
     3421 ccgatagcgg cagcagaaag cagttccaac acatggtcac ggtcagaaac gctgtcgaga
     3481 ctgttacggg tcgccgaagc aaagcccagc caacctgcta actgttcacg gtcgatttca
     3541 taggccgttc ctgccagtgc gccacagcct agcgggctga catccagacg cttaagcgca
     3601 tcctgcaaac ggctttcatc acgcgccagc atctcaacgt aggccaggca ccagtgcgcg
     3661 aacgtcaccg gctgggcgcg ttgcaggtgg gtgtaacccg gcatcaccgc gtcctgattg
     3721 ttctgcgcgg tctcgaccag agcggattgt aactgccggt tagccgtcag caactcgcta
     3781 acggtatctt tgcaccacag tttcaggtca gttgctacct gatcattacg gctacgcccg
     3841 gtatgcaatt ttttgcctaa ctgacccact ttgtcgatca gtttgccttc cacccagcta
     3901 tggatatctt cggcgtcgct ttcaaggatt tgttgtggcc tggcgcgaac atcttccagc
     3961 agcacgttca gcgcctcttc cagttgcgcc tgctcttctg cggttaacac gccgaccgtt
     4021 accagggctt tggaccaggc cacagagcca acaatatcct gctccgccag acggtaatca
     4081 aagcgcagtg agtcgttgaa ttgtttgaac cgttgatctg ctgcctgggt aaaacgcccg
     4141 ccccaaagtg ccataactct gtttccttat tttgaaattc aatgccggaa agctgcgcct
     4201 ccggccaaca aaacttaagc taaaatccgc gtccccatcg gcataccgtt aaacagtgcc
     4261 ggaagctgtt ccgcatgacg ccaggaggcg atatctaccg gacggcccaa cgtgcgcgca
     4321 gcatccagcg ccgcgttcac tttcacaatc atgccgtcag taataatgcc ctgctcaatc
     4381 agttgttctg ctttcgcggc ggtcatttcg gcaatacgtt gccctttgcc gtcgagaatg
     4441 ccgctgacgt cagagagcaa aatcagatcc gcgcccagcg ttgccgccag cgccgtcgcc
     4501 gcctggtcgg cattgacgtt catcagttgc ccttcgtctg ttacgccaat ggagctgacc
     4561 accggcagat aaccgttctc cagcaaactg ttgataagct taggcgaacc tggctgcgcc
     4621 agtccaacat ggcctaactc ttcatcaagc tgggtcactt tgatgctgtc gccgtcaccg
     4681 agaaacaaac ctacggccgc aatctgatgt ttcttcgccc atgccagcag ggttttattt
     4741 gccgttcccg ccagtgctcc ggtgataatg tctatctgat ccgcaggcgt cacccgcagg
     4801 ccatttttct ttttcaccgg cagattcagc cctttcatca gctcatccac cacacaaccg
     4861 ccgccgtgca caatcaccag cggacgctga tgtgactcgc gataattcac cagtgcgcta
     4921 aacagacgtt ccagcgcctc ttcactatcc agcagtacgc cacccagttt gataattaat
     4981 ggattcatca ttgcaccctt aaataagaga ctgcgtttcc gcatagccga aacgaatatt
     5041 ggcgcactgc accgcttgtg ccgccgcgcc tttcagtaag ttgtcttcgg tcgccacaat
     5101 aatcagatgc tcgccctgaa cggcaaaacc gatatcgcag aatggcagtc caacgacatt
     5161 tttcagcgcc ggaacgcctt tgtcatacag ccgcaccagt ggtttatggg catacgcctg
     5221 ctgtaacgct tgcgccactt gcacctggct cacacccggt ttcaggcggc aggtaatggt
     5281 ttcgagaatg ccgcgcggga aattgcccag atgcggggtg aagataacgt cagcaccgag
     5341 gtgtgtggcg atctctggtt gatggcgatg ggtaaagacg ccatacggtt gcaggctaac
     5401 ttcacaaaaa ctatttgaaa tcgctgcctt acgtcctgca ccgctcacgc cgctggtggc
     5461 gttgatcacc ggccactgat tgaggtcaag aagatcggca tcaatcaacg gtttcagcgc
     5521 cagctgtgct gccgtcggat aacagcctgg cacggcaatc aagttcgctt cttttaattt
     5581 attaccgcac cactccgcca gaccgtaggc tgcctgttcc agcagttccg ggtattgatg
     5641 ggtaaagccg taatattttt catagaaggc gacatcgtta acacgaaacg cgccggaaag
     5701 gtcgaacacc acacagcctg cttcaagaaa ttgcggcgct aaatcgtggc tgacttcatg
     5761 ggcggtggcg agaaacacca cgtccacccc tgggctaaac tcgccgatat ccgacatcgg
     5821 ctgcaacggc agatcaacga tgccttttag ctgcggatgc aaatcggaga ttaactttcc
     5881 cgcatcattg ctttgcgctg aaacagtcaa agcggttatg ttcatatgcg gatggcgatt
     5941 tacataggtc actagctctg cgccagcgta gccactggca cccacaatca gcgtattcaa
     6001 catcggggct attcaccttc ttatgtctgg ttgccaggtt aaacgtaaaa cattcacctt
     6061 acggctggtg ggttttatta cgctcaacgt tagtgtattt ttattcataa atactgcatg
     6121 aatattgata ctatcatgac cagaggtgtg tcaacaatga aaaacaaatt accgccattt
     6181 atcgagattt accgtgctct gattgccaca ccttcaataa gcgccacgga agaggcactc
     6241 gatcaaagca atgcagattt aatcactctg ctggcggact ggtttaagga tttaggcttc
     6301 aatgtggaag tgcagtctgt gccaggaact cgcaacaaat ttaatatgct ggccagttgc
     6361 ggacaggggg ctggcggttt gttgctggcg gggcataccg atacggtgcc atttgatgac
     6421 ggacgctgga cgcgcgatcc atttacgttg acggagcatg acggcaagct ttacggctta
     6481 ggcaccgccg acatgaaagg cttttttgcg tttatccttg atgcgctacg cgatgtcgac
     6541 gtcacgaagc ttgcaaaacc gctctacatt ctggcgactg ccgatgaaga aaccagtatg
     6601 gccggagcgc gttattttgc cgaaactacc gccctgcgcc cggattgcgc catcattggc
     6661 gagccaacgt cgctgcaacc ggtacgcgca cataaaggcc acatctcaaa tgccattcga
     6721 attatgggtc agtcgggcca ctccagcgat ccgtcgcgcg gggttaacgc tatcgaactg
     6781 atgcacgacg ccatcggcca tattttgcaa ttgcgcgata acctgaaaga acgctatcac
     6841 tatgaagcgt ttaccgtgcc ataccccaca ctcaacctcg ggcatattca cggcggcgac
     6901 gcctctaacc gtatttgcgc ttgttgtgag ttgcatatgg atattcgtcc gctgccaggc
     6961 atgacactca atgaacttaa tggtttgctc aacgatgcat tggctccagt gagcgaacgc
     7021 tggccgggtc gtctgacggt cgacgagctg cacccgccga tccctggcta tgaatgccca
     7081 ccgaatcata aactggttga agtggttgag aaattgctcg gagcaaagac cgaagtggtg
     7141 aattactgta ccgaagcgcc gtttattcaa acgttatgcc cgacgctggt tctggggcct
     7201 ggctcaatta atcaggctca tcagcctgat gaatatctgg agacgaggtt tatcaagccc
     7261 acccgcgaac tgatcaccca ggtgattcac catttttgct ggcattaaaa cgtaggccaa
     7321 acaatgtcgg atgcgatgct tgcgcatctt atccgaccta cacctttggt gttactcgag
     7381 gcgatttttt aacattccca taagttacgc ttatttaaag cgtcgtgaat ttaatggcgt
     7441 aaattcctgc tatttattcg tttgctgaag cgatttcgca gcatttgacg tcaccgcttt
     7501 tacgtggctt tataaaagac gacgaaaagc aaagcccgag catattcgcg ccaatgcgac
     7561 gtgaaggata cagggctatc aaacgataag atggggtgtc tggggtaata tgaacgaaca
     7621 atattccgca ttgcgtagta atgtcagtat gctcggcaaa gtgctgggag aaaccatcaa
     7681 ggatgcgttg ggagaacaca ttcttgaacg cgtagaaact atccgtaagt tgtccaaatc
     7741 ttcacgcgct ggcaatgatg ctaaccgcca ggagttgctc accaccttac aaaatttgtc
     7801 gaacgacgag ctgctgcccg ttgcgcgtgc gtttagtcag tttctgaacc tggccaacac
     7861 cgctgagcaa taccacagca tttcgccgaa aggcgaagct gccagcaacc cggaagtgat
     7921 cgcccgcacc ctgcgtaaac tgaaaaacca gccggaactg agcgaagaca ccatcaaaaa
     7981 agcagtggaa tcgctgtcgc tggaactggt cctcacggct cacccaaccg aaattacccg
     8041 tcgtacactg atccacaaaa tggtggaagt gaacgcctgt ttaaaacagc tcgataacaa
     8101 agatatcgcc gactacgaac acaaccagct gatgcgccgc ctgcgccagt tgatcgccca
     8161 gtcatggcat accgatgaaa tccgtaaact gcgcccaagc ccggtagatg aagccaaatg
     8221 gggctttgcc gtagtagaaa acagcctgtg gcaaggcgta ccgaattacc tgcgcgaact
     8281 gaacgaacaa ctggaagaga acctcggcta caaactgccc gtcgaatttg ttccggtacg
     8341 ttttacctcg tggatgggcg gtgaccgcga cggcaatccg aacgtcactg ccgatatcac
     8401 ccgccacgtc ctgctactca gccgctggaa agccaccgat ttgttcctga aagatattca
     8461 ggttctggtt tctgaactgt cgatggttga agccacccct gaactgctgg cactggttgg
     8521 cgaagagggt gccgcagaac cgtatcgcta tctgatgaaa aacctgcgtt ctcgcctgat
     8581 ggcgacacag gcatggctgg aagcgcgcct gaaaggcgaa gaactgccaa aaccagaagg
     8641 tctgctgaca caaaacgaag aactgtggga accgctctac gcttgctacc agtcacttca
     8701 ggcgtgtggc atgggtatta tcgccaatgg cgatctgctc gacaccctgc gccgcgtgaa
     8761 atgtttcggc gtaccgctgg tgcgtattga tatccgtcag gagagcaccc gccataccga
     8821 agcgctgggc gagctgaccc gctacctcgg tatcggcgac tacgagagct ggtcagaagc
     8881 cgacaaacag gcgttcctga tccgcgaact gaactccaaa cgtccgcttc taccgcgcaa
     8941 ctggcaacca agcgccgaaa cgcgcgaagt gctcgatacc tgccaggtga ttgccgaagc
     9001 gccgcaaggc tccattgccg cctacgtgat ctcgatggcg aaaacgccgt ccgacgtgct
     9061 ggctgtccac ctgctgctga aagaagcggg tatcgggttt gcgatgccgg ttgctccgct
     9121 gtttgaaacc ctcgatgacc tgaacaacgc caacgatgtc atgacccagc tgctgaatat
     9181 cgactggtat cgcggcctga ttcagggcaa acagatggtg atgattggct attccgactc
     9241 agcaaaagat gcgggcgtga tggcagcttc ctgggcgcaa tatcaggcac aggatgcatt
     9301 aatcaaaacc tgcgaaaaag cgggtattga gctgacgttg ttccacggtc gcggcggttc
     9361 cattggtcgc ggcggcgcac ctgcccatgc ggcgctgctg tcacaaccgc caggaagcct
     9421 gaaaggtggc ctgcgcgtga ccgaacaggg cgagatgatc cgctttaaat atggtctgcc
     9481 agaaatcacc gtcagcagcc tgtcgctgta caccggggcg attctggaag ccaacctgct
     9541 gccaccgcct gagccgaaag agagctggcg tcgcattatg gatgaactgt cagtcatctc
     9601 ctgcgatctc taccgtggct acgtacgtga aaacaaagat tttgtgcctt acttccgctc
     9661 cgctacgccg gaacaagaat tgggtaaact gccgttgggt tcacgtccgg cgaaacgtcg
     9721 cccgaccggc ggcgtcgagt cgctgcgcgc cattccgtgg atttttgcct ggacgcaaaa
     9781 ccgcctgatg ctccccgcct ggctgggtgc aggtacggcg ctgcaaaaag tggtcgaaga
     9841 tggcaaacag agcgagctgg aagccatgtg ccgcgattgg ccattcttct cgacgcgtct
     9901 cggcatgctg gagatggtct tcgccaaagc agacctgtgg ctggcagaat actatgacca
     9961 acgcctggtc gacaaagcac tgtggccgtt aggtaaagag ttacgcaatc tgcaagaaga
    10021 agacatcaaa gtggtgctgg cgattgccaa cgattctcac ctgatggccg atctgccgtg
    10081 gattgcagag tctattcagc tacggaatat ttacaccgac ccgctgaacg tattgcaggc
    10141 cgagttgctg caccgctccc gccaggcaga aaaagaaggc catgaaccgg atcctcgcgt
    10201 cgagcaggcg ttaatggtca ctattgccgg gattgcggca ggtatgcgta ataccggcta
    10261 atcttcctct cctgcaaacc ctcgtgcttt tgcgcgaggg ttttctgaaa tactgctgtt
    10321 ctaacaccct cgttttcaat atatttctgt ctgcatttta ttcaaaatct ggatatccct
    10381 tcagatatcc ttaaggaatt gtcgttacat tcggcgatat ttttttcaag acaggttctt
    10441 actatgcatt ccacagaagt ccaggctaaa ccccttttta gctggaaagc cctgggttgg
    10501 gcactgctct acttttggtt tttctctact ctgctacagg ccattattta catcagtggt
    10561 tatagtggca ctaacggcat tcgcgactcg ctgttattca gttcgctgtg gttgatcccg
    10621 gtattcctct ttccgaagcg gattaaaatt attgccgcag tgatcggcgt ggtgctatgg
    10681 gcggcctctc tggcggcgct gtgctattac gtcatctacg gtcaggagtt ctcgcagagc
    10741 gttctgtttg tgatgttcga aaccaacacc aacgaagcca gcgagtattt aagccagtat
    10801 ttcagcctga aaattgtgct tatcgcgctg gcctatacgg cggtggcagt tctgctgtgg
    10861 acacgcctgc gcccggtcta tattccaaag ccgtggcgtt atgttgtctc ttttgcccta
    10921 ctttatggct tgattctgca tccgatcgcc atgaatacgt ttatcaaaaa caagccgttt
    10981 gagaaaacgt tggataacct ggcatcgcgt atggagcctg ccgcaccgtg gcaattcctg
    11041 accggctatt atcagtatcg tcagcaacta aactcgctaa caaagctact gaatgaaaat
    11101 aatgcattgc cgccgctggc taatttcaaa gatgaatcgg gtaacgaacc gcgcacctta
    11161 gtgctggtaa ttggcgaatc gacccagcgt ggacgcatga gcctgtacgg ttatccgcgt
    11221 gaaaccacgc cggagctgga tgcgctgcat aaaaccgatc cgaatctgac cgtgtttaat
    11281 aacgtggtta cgtctcgtcc gtacaccatt gaaatcctgc aacaggcgct gacctttgcc
    11341 aatgaaaaga acccggatct gtatctgacg cagccgtcgc tgatgaacat gatgaaacag
    11401 gcgggttata aaaccttctg gatcaccaac cagcagacaa tgaccgcccg caatacgatg
    11461 ctgacggtat tttcgcgcca gaccgacaag cagtactaca tgaaccagca acgtacgcag
    11521 agtgcgcgtg aatacgacac caacgtgctg aagccgttcc aggaagtgct gaaggaccct
    11581 gcgccgaaga aactgatcat cgttcatctg ctgggtacgc atatcaagta caagtaccgt
    11641 tatccggaag atcagggcaa atttgacggt aatacagagc atgttccacc gggattaaac
    11701 gcagaagagc tggagtcata taatgattat gacaatgcta acctgtacaa cgatcacgtg
    11761 gttgccagcc tgattaaaga ctttaaagcg accgatccga acggtttcct ggtttatttc
    11821 tctgaccacg gtgaagaggt ttacgacacg ccgccgcaca aaactcaggg gcgtaatgaa
    11881 gacaatccga cgcgtcatat gtacaccatt ccgttcctgc tgtggacgtc agaaaaatgg
    11941 caagcgactc atccgcgtga tttctcacag gatgttgatc gtaaatacag cctggcggaa
    12001 ctgatccaca cctggtcaga tttggcgggc ttatcttacg acggttacga tccaacccgt
    12061 tcagtggtga atccgcagtt caaagaaact acccgctgga ttggtaaccc gtataagaaa
    12121 aacgcactga tcgattacga cactctgccg tatggcgatc aggtgggtaa tcagtaatta
    12181 tcagccagat gttatttgcc ggatgcgacg cttgacgcgt cttatccggc ctacagatcg
    12241 cggcggtagg ccggataagg cgtttacgcc gcatccggca atacaagtcg actgaaactg
    12301 ccgcaatgtt tacatctcac tcccatcagg ccacactttt caggtggcct tttttcataa
    12361 tgccagttac tgttttcccg tctacatacc taagggaata acatgtatca cgacgtcagc
    12421 tatctgctct cccgcctgat caacggcccg ctgtccctgc gccagattta ctttgccagc
    12481 agcaacggcc cggtgcccga tcttgcgtat caggtcgatt ttccacgtct ggaaattgtg
    12541 ctggaaggtg agtttattga taccggcgct ggagcagcgt tagttcccgg cgatgtgctg
    12601 tacgtccctg ctggtggctg gaattttcca caatggcaag cccccgccac gacctttagc
    12661 gtgctatttg gcaaacagca actcggcttc agcgtcgtgc aatgggatgg caaacaatat
    12721 caaaatctgg cgaagcaaca cgtcgcccga cgcggcccac gcattggttc ttttctgcta
    12781 caaacgctca atgaaatgca aatgcagtcg caggagcagc aaacggccag acttatcgtc
    12841 gccagcctgc ttagccactg ccgcgatttg ctcggcagcc aaatacagac cgcctcacgc
    12901 agccaggctc tattcgaagc tattcgtgat tatatcgacg aacgctatgc ctccgcactt
    12961 acccgcgaat ctgtcgcaca ggcgttctat atttcgccaa attacctctc gcacctgttt
    13021 caaaaaacgg gggccattgg ttttaacgaa tacctgaatc acacgcgact ggagcacgct
    13081 aagacattat tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt
    13141 gtcgacagca actacttctg ccgtctgttt cgtaaaaata ccgaacgctc gccgtcggaa
    13201 tatcgtcggc agtaccacag ccaactgaca gaaaaaccga ctactccaga ataagatggg
    13261 tttgctgcgg cgcagaaagc actttgcgca ctgcgctcat caccctctgc ggctcacgca
    13321 ggaaagcgta gatgctgcat tgcacgtagc gacaatgttc aaatcgctca gcaccggcaa
    13381 gctcgatatc cgtaattaac agcgcaacat ccgcccggcg gatatcctcg tccgctaaac
    13441 gattctccgt tcccaacgcg ccctgagttt caatgctgac gccccacttc tctaactggc
    13501 acaacttttc cagccgttcc gccgccatat aagtatgcgc cacgccgctt acgcaggcgg
    13561 ttactgccac cagatatgcc attttaacct cccacggtaa cctgaaatcc ggcccgttct
    13621 gccatttcac gcatcgttgc cacatcggct gacgacggcg cagcaacctc tttcatcgac
    13681 catgttttcc ccagcaggcg gtatttcggc tcgccgtact gatgaaacgg taacagatgg
    13741 atctgcttga tattcagcgg gatcagcacg tccagcgcct gctgcatatt ctcccggctg
    13801 agcgtgaaac cagggatcag cggtaaacgc gggatcacgt tgacaccctc actcaccagc
    13861 aaacgcagat tctccagcac gcgttgcagg ttcatcttca ccacatcccg cgcctgagcc
    13921 gcatccataa tttttaaatc gaacaacact tcatcgcaca atttcgccag cggtaacagc
    13981 ttgctggctg gcgcgtctcc ggcagtttcg atggcgcatg acacacccca tagccgcagt
    14041 cgctgtaaaa aacgggtcgc aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg
    14101 acgccgccgc cggacgtgcg aaagaaaatg tcatctttca tcacttcccg ttccagcgcg
    14161 tcaaggctga tatcgcggcc aatccgttca aatgccccgg aggggcattc atccgcatca
    14221 cgcaaacatt tcgcgcagtg cagacatttc gcctctctgc gcaccgtctg gattttgccg
    14281 gagatcgact ccggattagc acaccatggg caaagatgcg gacagccttt aaaaaagacc
    14341 accgtacgaa tgccctcgcc gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc
    14401 acatcatcgc ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc ggatgatgtc
    14461 atcctggatc tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac
    14521 cagcccggca taatcttgtg ggcgctgttg cgcttcccgt aacgtgtcgg cgttcaccac
    14581 gttaaactga atatgctgca acttaagctg ggtaaacgcc cgtaagaagt cggccagttt
    14641 gcgtaatccc gcttcacctt cgagggtcgc cggagtgaat ttcacgttca gcaatgtacc
    14701 gttagaaagc agtgtgttat cgagcttact gactgatttc agtaccgccg ttggtccttg
    14761 tgcgtcctga cccagcatgg gtgacaagcc accgtctgcc agttgctctc cggcaaagcg
    14821 accgtctggc gtcgcgccaa ccaccgctcc caacgggaca tgagcagaaa cggtatacga
    14881 tcccggcgtg aagtagccgc cgcgcgggtt ctggtatttt tccacttctt tgcagtagtg
    14941 gcgtaacagt tcggcgctaa tgttatccac ctcgtcgata tcgttaccgt atttctcgaa
    15001 gcggttaatt aagcgagcgc ggactttttc gccttctggc gtggcgaagt tggcttttaa
    15061 taccgacagc aattcgtcaa aacttaaacg ctgttgatca aaaatcatcc ctttcaacgc
    15121 atggagagaa tcgctcaggt tggcgatacc gatcccctgt acgccggaga agttataacg
    15181 cgcgccgcca tcggtaatat cgcgaccttt ttccagacaa tcgctgataa aagacgagag
    15241 tagcggtaca ggtgcccagt cgcgatggcc gatatcacaa atattgctgc cctcaaccat
    15301 cagggtgatg tagtggctga tcttggcgcg gatctgttcc agtaaacctt cataagtcag
    15361 cgcagcgttg ccttcatttt catgcaggca gatttccatc actttcagca ggttaaacat
    15421 cgcgatgtca tgcaagccgt aggttctgcc gggaatagat aattccacac agcccactac
    15481 ggaatagtcg cgcgcatctt ccagcaaaac gccacggttg aggaacgctg gcactaccac
    15541 ttcatcgtta aagatttgcg gaataccggt gccgaggcga atggtttctg cggttttcat
    15601 caggaacggc gtgtcgatca aggcgttagt gcgcacgccg aggttcggtt gcggtaattg
    15661 cacgctttga taagcgtcaa ggcaaaggaa cgaaagcacg ttcaccgcgc tacgtccgtt
    15721 ctcggttaac ccgccgagca gtgcggtata gccggtaggg aaaccggcga aataacgcgc
    15781 gctactggtg gagcgcaaca gcacgatgtc gttgcatttc acccataaag attcgagcag
    15841 ttctttcagg aatgccggat cttcgccctg ggttaatgat gtctgataga acggcaacat
    15901 atactggtcg aagcgcccca acgatagcga actggcgttg gattcgtatt gcagaatgat
    15961 gttcatgtac cagaataact ggcacgcctg ccagaacgtc tgcggcttat gttcggcgtt
    16021 atggcgggag atctccgcaa tagtcagcaa ttcttcgcga cgcgggccat cagtgcagct
    16081 tgccgccatc gtttccgcca gttcggcgta acgcaaaata tgtttctgcg aggcttccag
    16141 cagtaacagc gctgcctgat aaaagtgatt ctccggctgt tgctgacaat gttgctgcat
    16201 ctgtgccacc agctccccca gcccgtgatt cagcaggcgc gggtaatcaa taataatgtg
    16261 cccctggcct ttatccgtct ggttgatgct gaaaatctgc gtgctggtcg cggcttttac
    16321 ttcatccgtc atctgcccgt tgatgaaatc tttcatcgaa cgtttttccc agtacgggaa
    16381 caactcttcg cggtagatac gtttatcttc ttcgctgata gcaaagcggt cctgcggacg
    16441 cgtcgggaat tgatccagtt ctttcagcag ccagtaaggg tccatttccg gcgacataat
    16501 cccggcgcgt ggttttacgg tgcggttacc ggcaatcagt tcttcatcac gaatcgaaat
    16561 ttcaacgtgt tcaaggatat acgctgttgc tttcgcccga cgcattatca ccggctcccc
    16621 ttcggtttgc cgatggctgg cggtataaag caacgcccgc tccagcgaga tttcacgggt
    16681 attggcaaac agtgcagttt tgaggcgaga gatacgattc gtcatgagga actcctgtgg
    16741 gaaagcgaac ccgcccttgc gggcgggata agaacttaag cggtttgtgc caggtgagct
    16801 tcaattttgc tcataatggc gtcagcacgc tttaccgcgt cgctgatgtt gacacgcacg
    16861 atagtcttgc cagcaaaacg ctcttcaaat ttgatgccga tatctttggt caaaatcacc
    16921 atatcggcgc ttgccacgtc ttccgcagtc agttcatttt ccagaccaat cgacccctgg
    16981 gtttctactt ttacttccca gcctttcgct ttcgcggcgc tttccagcgc ctctgccgcc
    17041 atataagtat gggcaacacc tgaaggacat gcggttactg cgataatttt cgtcatggct
    17101 cgttcctcaa ttaattaatt tcaaaatcca gatccaggtc gtcttccttc tcttcagttg
    17161 aagacccatt tttacgcgcc agacttttca gcacgttcac gcagactgcc gtcacaaccg
    17221 ccccgacagc cactgccgcg atgtaaccca acttaccttc aaccaccggc agcacaatca
    17281 gaccacccca gcctgcatag cactgcgcac cgaccagcgc cgcagttacc gcaccacaaa
    17341 ctgaaccgac catgatggaa ggaatgacac gcagcggatc ggcagcggcg aaaggaatcg
    17401 ccccttcagt aacgccaaca cagcccatca ccagtgccgc cttgccggtt tcgcgctctt
    17461 ctgcggagaa atttttacga ccaatcagcg tcgccagccc catccccagc ggtggaacac
    17521 aaatcccaac agcggcaata gccaccacgg tataaacacc ctgagcaacg caaatcagca
    17581 tgaaggcata ggccactttg ttaaccgggc cgcccatatc gaacgccagc atcagaccca
    17641 tgatcaccgc cagcataaca atgctgccct gctgcatccc ctgaagccac tgggtcaggc
    17701 tgttggtcaa cgcccccacc ggctcgccca gcccccacat catgacgcca gcggtaatca
    17761 gtgtgccgac gattggaatg atgaagattg gcattaccga gcgcagaact ttatgcactg
    17821 gaattttctt caggtaatgc accacgatgc cgccgataat cccggcgatc agcgcaccaa
    17881 agaagcccgc gccaaagctg ttaccaaccc aggcaccgat agcgcatgga gccagcgcag
    17941 aacgttctgc aatggaataa ccgatgtaag cggcgaggaa aggcaccatc agcgtcaaac
    18001 ctgcaacgcc gatatcaaac agttttttca gattcggatc ggctacggca tccggcactg
    18061 cgcctttgcc atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg
    18121 gaatcatgtg tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca
    18181 actcattcat aagctgctcc cgggcttgcg aaagcccatg tccatatttt tgatggaaga
    18241 aacaataggc aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt
    18301 gtaatatcag tacaaaaatg cgatccgcct cataacttgc gataatccgg aagtacgccg
    18361 caaaacggcg tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt
    18421 ttggcagatt tgtcacatgg caggccgttg ccgtgcgctc ttatcctgta aacagaaaac
    18481 cgtaccggga gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc
    18541 gtccatgcgc gtccggcaag ccacgttgaa acgctgtgta atactttttc atcacaaatt
    18601 gagtggcata acctgcgcac tgaccgcaag ggcaacgcca aaagcgccct tgcgctgatt
    18661 ggcaccgata cgctggtggg cgataactgc cagttactga tttccggggc cgacgaacag
    18721 gaagcgcacc agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgacgcaccg
    18781 ctggcggaag taaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat
    18841 ccgcaaatta tccgcgctcg caccgtgtgc agcggtagcg caggcggcat tctgacgccg
    18901 atctcttctt tagatctcaa tgcgctgggt aatcttcccg cagccaaagg cgttgacgcc
    18961 gaacaatccg cactggaaaa cggtctgacg ctggtactga aaaacataga gtttcgtctg
    19021 ctggatagcg acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat
    19081 acttccctgc gcgaacattt actggcaggt gtcagcgccg gattaagctg cgccgaagcg
    19141 attgttgcca gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagttacctg
    19201 caagaacgtg ccctggacgt acgcgacgtc tgcttccagt tactccagca aatctacggt
    19261 gagcaacgct tcccggcacc gggcaaactg acgcagcccg ccatttgtat ggctgatgaa
    19321 ctgaccccca gccagttcct cgaactggat aaaaatcatc tcaaaggtct gttactgaaa
    19381 agtggcggca ccacatcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg
    19441 gtaggtgtgg atattgatgc ccttactccg tggcagcatc agacgattta tatcgacggc
    19501 aacgcagggg cgattgtggt tgagccaggg gaatccgtgg cccgttatta tcagcaagaa
    19561 gcccgcgtac aggacgctct gcgtgagcaa cagcgtgtct ggctgaccca gcaagcgcgt
    19621 accgctgacg gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag
    19681 gccgcattcg gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg
    19741 gatcgcacca gtgcaccggg tgaaagcgag ctgtacaaca tcttttgtca ggcgctggag
    19801 tctgccaacg gacgcagcat tattgtgcgc actatggaca ttggtggtga caaacccgtt
    19861 gattatctga acattcccgc agaagctaac ccgttcctcg gttatcgcgc ggtgcgtatt
    19921 tatgaagaat acgcgtcgtt gtttaccaca caactacgat cgatcctgcg cgcctctgct
    19981 cacggcagcc tgaaaatcat gatcccgatg atctcctcaa tggaagagat cttatgggtg
    20041 aaagaaaaac tggcggaagc caaacagcaa ctgcgtaacg aacacattcc gtttgatgag
    20101 aagatccagc tcggcatcat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc
    20161 tgcgaagaga ttgatttctt tagtattggt agtaatgacc tgacgcaata tctactggca
    20221 gtggatcgcg ataacgctaa ggtgactcgt cactacaaca gcctgaatcc ggccttcttg
    20281 cgggcgctcg attacgccgt gcaggcggtg catcgccagg gcaaatggat tggtctgtgc
    20341 ggtgaactgg gagcgaaagg ttccgtgctg ccgttgctgg tcggtttagg gctggatgaa
    20401 ctcagcatga gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc
    20461 cgtgagtgcc gccagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa
    20521 cacctgctgg cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc
    20581 atcacactgg aaagcgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac
    20641 ctgctgctgg cgggccgctg ccgctatccg cgtaaactgg aagccgactt gtgggcgcgc
    20701 gaggccgttt tctctaccgg actgggcttt agtttcgcca ttccccacag taaatcggaa
    20761 cacattgagc aatccaccat cagcgtagcg cgtctgcaag cgccagtgcg ctggggcgat
    20821 gatgaagcgc aattcatcat tatgttaacc ctgaacaaac acgccgcggg cgatcaacat
    20881 atgcgcattt tctcgcgcct cgctcgtcgc atcatgcacg aagaattccg taatgcgctg
    20941 gttaacgccg cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa
    21001 aaggaaacat catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg
    21061 cacgcatatt cccgattgcc ggggtgacaa ctaacccgag cattatcgct gccagcaagg
    21121 agtccatctg ggaagtgctg ccgcgcctgc aaaaagcgat cggtgatgag ggcattctgt
    21181 ttgctcagac catgagccgc gacgcgcagg gtatggtgaa agaagcgaaa cgcctgcgcg
    21241 acgctattcc gggcattgtg gtgaaaattc cggtaacctc tgaaggtctg gcagcaatta
    21301 aaatgctgaa gaaagaaggc attactacgc tgggaaccgc agtgtacagc gccgcgcaag
    21361 gattactggc ggcgctggct ggagccaaat acgttgctcc atacgttaac cgcgtagatg
    21421 cccagggcgg tgacggcatt cgtactgtac aggagttgca agcgttactg gaaatgcatg
    21481 cgccagaaag catggtgctg gctgccagct ttaaaacacc acgtcaggcg ctggattgtt
    21541 tgctggcagg atgtgaatcc atcacactgc ccttagatgt agcgcaacaa atgcttaaca
    21601 cccctgcggt agagtcagct atagagaagt tcgagcacga ctggaatgcc gcatttggca
    21661 ctactcatct ctaaaggagt aattatggac cgcattattc aatcaccagg taaatacatc
    21721 cagggcgcag atgtaattaa tcgtctgggc gagtacctga agccactggc ggaacgctgg
    21781 ttagtggtgg gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt
    21841 aaagatgctg gactggtagt agaaattgcg ccgtttggcg gtgaatgttc gcaaaatgag
    21901 atcgaccgtc tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtattggt
    21961 ggcggaaaaa ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg
    22021 atcgcaccga ctatcgcttc taccgacgca ccgtgcagcg cattgtctgt tatctacacc
    22081 gatgagggtg agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc
    22141 gacaccaaaa tcgtcgctgg cgcgcccgcg cgtctgttag cggcgggtat tggcgatgcg
    22201 ctggcaacct ggtttgaagc gcgtgcctgt tcccgtagcg gcgcgaccac catggcgggt
    22261 ggcaagtgca ctcaggcagc gctggcactg gctgaactgt gctacaacac cctgctggaa
    22321 gaaggcgaaa aagcgatgct tgccgccgaa cagcatgtag tgactccggc gctggagcgc
    22381 gtgattgaag cgaacaccta tttgagtggc gttggttttg aaagcggcgg gctggcggcg
    22441 gcacacgcgg tacataacgg cctgaccgct atcccggacg cgcatcacta ttatcatggt
    22501 gaaaaagtgg cattcggtac gctgacgcag ctggttctgg aaaacgcgcc ggtggaggaa
    22561 atcgaaaccg tagctgcgct tagccatgcg gtaggtttgc caataactct cgctcaactg
    22621 gatattaaag aagatgtccc ggcaaaaatg cgaattgtgg cagaagcggc atgtgcagaa
    22681 ggtgaaacca tccacaacat gcctggcggc gcgacgccag atcaggttta cgccgcactg
    22741 ctggtagccg accagtatgg tcagcgtttc ctgcaagagt gggaataatc tactccaaac
    22801 tcccggcttg tccgggagtt tgaacgcaaa attgcctgat gcgctacgct tatcaggcct
    22861 acgcaatctc tgcaatatag taaatttacg tacttttgta gaccggataa ggcgttcacg
    22921 ccgcatccgg cacgctgtgc cagtaatctg aaacttccac gttgttcggg tgttttttta
    22981 tccgcttctg gacattttct ccacagaaca atcgttagcc cggaaggtcg aatctgtgac
    23041 tcttattttc acggacgaaa cagatgaaag cttcactggc gttattcagt cttctgacgg
    23101 tatttaccag ttactcctta aaatctcccg ccgttccacc aaccgtggta caaatccagg
    23161 ccaacaccaa tcttgccatc gcagacggcg ccagacagca aatcggcagc acgctatttt
    23221 acgatccggc gtatgtgcag cttacctatc ctggcggtga tgtcccgcaa gaacgcggcg
    23281 tatgttccga tgtggtgatc cgtgcattgc gcagccagaa ggtcgatttg cagaaactgg
    23341 tgcacgaaga tatggcgaag aattttgctg cgtacccgca aaaatggcag ttaaagcgcc
    23401 cggacagcaa catcgatcac cgtcgggtgc ctaatctgga aacctggttt acccgccacg
    23461 ataaaacacg ccccaccagc aagaacccca gtgactatca agcgggcgat attgtctcct
    23521 ggcgattaga taacgggctg gcacatattg gggtggtgtc agatggcttc gcccgcgacg
    23581 gtacgccgct ggtgatacat aacattggcg caggcgcgca ggaagaagat gtactgttta
    23641 gctggcggat ggtggggcac tatcggtatt ttgctaaata agagatctcc ccacatgaag
    23701 aaaaaattca gatccttttt acactgacgg cttctttttt cttacggctc accagcgcca
    23761 gtgccagtac aatcagcaca ataccgctac cttcaactgc ccccggattc tcaccgagca
    23821 gccaccagga aaagagtacg ccgcaaacgg gaacggccag tgtgcttaag ctggcaatac
    23881 tggcaggcaa gtttttcaat acaaacaacc ataagctcca cgccagcgct gtcgccagaa
    23941 tcgcactgta ggccagcgcc cagaacacgg tgggttgcca gtcaatttca cgttgcggca
    24001 ccagtaaagc gaccacgctc atcaccagcg ccgcatacag catctgccag gatgttagcg
    24061 acaataaatc cacgcgcgga tggcgggcat acagacgttt agcaacaatc gcgctcgccc
    24121 cccaactcac accggagagg attgccagca tggcactttt catcgaagag aaatcgagtt
    24181 gccacggctg caacaccaaa aataagccga aagcggcaat cagtatcgcg aaatattgcc
    24241 cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat caccacccag aacggcatgg
    24301 tatagctcag gattgccacc ttccccgctc cgccgctgac cagcgcccac tgtgccaggc
    24361 caaccatccc gcaggtttgt aacagggcaa tggctaaggt gtatttaaac ggcgtcgggc
    24421 gcattccgcg accacgtaat aaaagaacga tgaataaaac gagagcgccg aaaatgcagc
    24481 gtaaggcggt gaagtcgaag gcaccgatgt agctggtgac ttgcttcatg aaaatccagc
    24541 tataactcca gataagtgtg agcacaacca ggccactgat tgccagtggg ttgctctttc
    24601 ctgcggcaga catgagagat ccagtgtgta gttttcctgt ggacactata cgccggtaaa
    24661 gaaacttcag cgactgcatg aaaggcagtc gctgaagaag gtcagattac agcaggtcga
    24721 aacggtcgag gttcatcact ttcacccacg ctgccacgaa gtctttaaca aacttctcgt
    24781 gggcatcgct gctggcgtag acttccgcca ccgcacgcag gacagagtta gaaccaaaga
    24841 ccagatccgc acggctggct gtgtatttca cttcgccagt ttcgcggtca cggccttcga
    24901 acagttcttt cgattcgtcg gtcgctttcc actcgtaacg catatccagc aagttcacga
    24961 agaagtcatt gctcaataca ccaacgcggt cagtgaagac gccgtttttg ctgccatcga
    25021 agttggcacc cagtacgcgc atcccgccca ccagcgcagt catttccggc gcggtcagcg
    25081 tcagttgctg tgctttatca atcaacagtg actcggtggt ggaaacgtcc agacgagcgc
    25141 gatagttacg gaaaccgtca gcaattggtt ccagcagttc gaacatctca atgtcagtct
    25201 gatcctgacg cgcatcaacg cgacccggcg caaacggtac atgaatgctc aaacctgcgg
    25261 cgcttgcggc tttctcaacg ccaaccacac ctgccagcac gatgatatcc gccagcgaag
    25321 ctttgccaga ctctttctga attttctcca gaactggcag agcacgaacg gctgcggcgt
    25381 tcacatccca gtcacgctgc ggcattaatg ccagacgcgc accgttggca ccaccacgtt
    25441 tgtcgccacc acggaaggta gaagcagacg cccaggccac cgataccagc tcactaacag
    25501 acagaccaga atccgcaatc gcgaatttca gatcgataat gtcctgctcg gtcgggttgt
    25561 agatcggctg cggcagcgga tcttgccaga tcagatcttc tttcggtact tccggcccga
    25621 tgtaacgaga tttcggcccc atatccctgt gcgtcagttt gaaccaggca cgagcaaagg
    25681 cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga gatcttctcg aactcaggat
    25741 caaaacgcag cgtcaggtcg gtcaccagca tcgtcggttt acgtttcttc gacggatcaa
    25801 acggatccgg gataatttcc ggtgcgtcta ccgcttcaaa ctggattgcg ccagccgggc
    25861 tgcgggtctg tacccactca tacttgaaca ggttctcgaa gaaatagttg ctccactggg
    25921 tcggcgtctg ggtccagacc acttccagac cagaggtaat ggcatctgcg ccaacgccgc
    25981 tgccgtaagt gctcgcccag cctaaacctt gttcttcaat cggtgcagct tctggatcag
    26041 gacctacatt tgatgtcgga ccggcaccgt gggttttacc cagcgtatga ccacccgcaa
    26101 tcagcgccac ggtttcttcg tcattcatgc ccatgttgcc gaaggtcgcg cggatagctg
    26161 ctgctgccga aagtggttcg ccgctgtgat ccgggccttc cgggttaacg taaatcaggc
    26221 ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc cggatgacgg tgagtcagcc
    26281 aggctttttc atcaccccag ttaacatcca gatccggttc ccagacgtct tcacgaccgg
    26341 caccaaaacc gaaggtacgg aagccggagt tttccagcgc cacgttaccc gcgaggataa
    26401 acaggtcggc ccaggagatt ttctgaccat atttctgttt gattggccac aacaggcgac
    26461 gcgctttatc gaggcttacg ttatccggcc aggagttcag cggtgcaaag cgttgctgac
    26521 cacgacccgc gccgccgcga ccgtcgattg aacggtaagt acccgcgccg tgccaggcca
    26581 tacgaataaa cagaccagcg tagctgcccc agtcggcagg ccaccacggc tgagattctg
    26641 tcagcagggc tttcagatct tttttcaggc cgtagtaatc taatttgctg aattctttgc
    26701 ggtagtcaaa gtcctcaccc agcgggttag aacgattaga atgttggttt aacaggtcaa
    26761 cacggagttg atttggccac cagtcgcgcg tggttgtgcc cccacctgcg ctctggtcgt
    26821 gaccgccctg atggaacggg catttgccgg tggctgtcgt gttatggata tcgtctgacg
    26881 tgctcatcaa tgtgctcccc tctacagtgt taccgttacg atacacagcg ttagagagaa
    26941 gttataattg aattaatcca cggatgcgat agttgagatc ttacatattg ttggttaaag
    27001 agatgtagat caaattgatc gtaattaaga ctttttgatt atgtccgatt taggacaaat
    27061 gctttataaa aaagggtttt agatttgtat ccttcaagta atgagggaga aaacaaggct
    27121 gataacctta ttttcccgcc ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg
    27181 tgaacactgt gtgattttca caaaaggcac aatacttata aaccaggtcg aactcccagt
    27241 gtatggcaaa tcgcataact catttcagcg cggttaagcg tatagaagtg gaaatctttc
    27301 actccttcac ggcttaaaat cttcaccata tccatggcga tattcgcgcc caccagcttg
    27361 cgggtttcag catcgtcatc caggccgtcg aacatttgtg ccatccacgc cggaatacgc
    27421 acgttggtca tgtcggcaaa tttcttcgcc tgtttaaagt tcgaaaccgg caaaattccc
    27481 ggaatgattt ccacatcaat gcctgccgag acgcagcgat cgcgaaaacg caggtagctt
    27541 tcgacatcga agaagaactg agtaatggcg cggttggctc cggcatccac ttttcgtctc
    27601 aggttgagta aatcggcctg ggcacttttc gcttctggat gtacttccgg atacgccgcc
    27661 acggagatat cgaaatctgc cacttctttt aacagcgtca ccaggtcaga agcatacatt
    27721 tctggcttac cacttcccgg cggcaggtcg ccacgcagcg ccacgatatg gcgaataccg
    27781 ttattccagt agtcgcgtgc aatgatccgc agctcggcag gcgtcgcatc aatgcaggta
    27841 agatgcggtg ccgcttccag gccggtgcga tctttaatgc ctttaataat gctgtgcgta
    27901 cggtcgcgct cgccggagtt tgcgccatag gtcaccgata caaacttcgg tttcaggctg
    27961 ctaaggcgat cgatggagtt ccacagggtc tgctccattt cactggtacg cggcgggaaa
    28021 aattcgaacg aaacgttaat ctgcccctgg acttctgcca gactctgatt cagggcatcc
    28081 cgctggctgg cgtgaaaaaa gctcatacct tacctcatca atcgcttatc gttatatgtt
    28141 gtgttgtgca catctatccg tttagacgtc cagatgtaaa gatgaaggaa aagccgaagg
    28201 gcgtcaaccg aaaaataacc aatgatggtg aggaaaaatc agggaagatg aaaaaaactc
    28261 atcttaatta gaggcgataa aagatttctc tatagcgcaa tccacctcgc acgttttgac
    28321 tcatattctg acaacacagc gacacctggg ctaattcgaa aaacaacgtc ctgccaccgg
    28381 atggtgatgt taaggcatct gattcggcct gcgccctcgc gtaggccgaa tacaggatct
    28441 gcgcaatcaa cgggtaacaa acagttgagt caattcagtg cgcactttta tttgatatcc
    28501 ttcacgcgca taccgttgag ctgttcatcc gtaatggtgt tgcccgcttt gaagtaatca
    28561 accaccgcgt gataaacgta ataaccgccc gttgtgttac gcgctttccc ttcggtaaag
    28621 gcggtaaaac catctccacc atcagcaagg aaactctgag tagcaatgtg ataaaccgtc
    28681 gcatcttcaa tgggtttgcc attcaaagtc agtgtgatta cccgctgacc aaccggcctg
    28741 ttgctgtcgt acttcatttc caggcctttc gatacctgta aaacgccatt gcttaaactt
    28801 gcgccgtgtt ccatcagact gcgtaattgt ttacccatga gttccatcgt caccagttcg
    28861 ttggggaacg gaaacgtact gatgacgccg cccatcgtga tcgcccctgc ggggatctca
    28921 ttgcggatac caccagagtt tgttaacgcc agttgggtgt ttttacccgc cgctaccaat
    28981 aaagcgtcag ccgccaggtt cccgagggaa gccgattcac cataggcacg ttttaattca
    29041 accggcgatt gcgccaccgt ttgttgcacc acttcatcaa gctttttatt ccagccgtcg
    29101 atcacctgtt tcgtttgcgg atcgggcttc cactcgtcag cgtaaatggt tttaagctcg
    29161 aagtttttca ccgtaaaatt atgcggcttc tctttgtagt cgagaaccag tttgcccaca
    29221 tcaatcccgc cgctgtcagt ggagaggatg agcgtattgc cgactttaat cggttccggc
    29281 gtacccacat gtgcatgtcc ggtgatcaaa atatccagcc ctttcacctg acttgccgtc
    29341 tgaatatctt tatccaacgc gcgacgcaca tctgtgcctc ccatactgga ctggcgggct
    29401 ggaacacctt cgtggatcag ggcgacggtc aaatcaacct tgcctttgag ttcatcgata
    29461 taacgttgta gccatttaat ttcatcgcgc gcttcaatac ccacgcgcgt ggcagcagat
    29521 accgtatcat taaaggcaaa tactccgtgc aaaccaataa cgcctatttt cacaccgtct
    29581 ttttcgatga tggtataggg cttatcccag aatgatttac tgctgttctg ataaaaaata
    29641 ttgccctgca caatagggaa ttttgcctgg ctcaactgta ataatgtgtt gtcccagccg
    29701 tggtcaaatt catggttgcc tattgtgacg gcatcaaacg gcatagtatt cataatatcg
    29761 ataatagcct tacctttggt caggctgctg atatatggcc cggtaaaata gtcgcccgca
    29821 tcaaaaaacc atgtcgcttt attttttgct ttttcttgct taaccagcgt agtgatattt
    29881 gcccagccgc caatatcccg tttaccgtca gcgatccacg gcactttata aggctcgaca
    29941 tgtgcatgga ggtcgttggt ataaatgatg gtgacatctt tagcgcaagc ccagaatggc
    30001 agtgttaatg cgatacccgc agcaagtatt ttaattttca taaacagtcc ctttcagtga
    30061 gataaaggat tgctacatta cgttctgaaa gacttattta tgtgagagta atcacatccg
    30121 caggggatag ttgccggaca aaatcaacga ccttgtgaaa tcgttggtga tatatgtcga
    30181 ctctgtttac gattaatgcc tgtaagtcgt ttggttgccg taatttaggg cagccaacat
    30241 cgacagatta tagctggccc gactatcgac tgggttatcc ggcactgcac tgtcgggcat
    30301 gtggcagtta cccgccgttg tttgacgagc aacaatttcg tgactggttg acagttcact
    30361 tatctacttt tgcaacagag aaaggccatt tttgcccggt atgttatggc acagagacga
    30421 tttgttatgg tcacaatcca cagggttcgc aacgcattca atgccgtaac tgcaaaaaag
    30481 tatggacacc caaacaatat cagaaagaaa taacgactcc cgaaataatc gaaacggttg
    30541 ctttgctcgt gccatttcag ggagtcagca gcgggcagaa actgtatgtt ttaatcagtt
    30601 ttgatgctct tcgcggtaat attcttcatc tttctactaa ttacacgcag catcaggctg
    30661 gagaaagtct gcattatcga tacagaggaa atgcagaacc agagttacac gacaataata
    30721 ttgtacaacg cgttgatatg cgcgaagcac aatttctacg ccgcagtcag tttgatgaaa
    30781 ttcagtatgg cagcgcagcg ctcaagcgca atgccaaagg cgctattctg cgtccggtta
    30841 tcactgcaca cgggcatttc agagtactga atattttatt ccctacagta aaaacacatg
    30901 taatttcaca cgagtgtttt ttaagaggtg ccattattac ggcctgggct gatctgtttc
    30961 gccaacagca aggtgaaatt tggtttattg aagaggaaat agctgacgat accgataata
    31021 tgccctggcg ttttcaggga aaaacttatc acggctggtg gaaaaatcag tggcaactct
    31081 gggttcaggg aaaaaatcgt aaaatggtat gtgcattaac gggcggaaag agtagcaaag
    31141 cagaaatgct ctctttagca accagcagac attttatcga ctggctacat aaacaagcag
    31201 tatttactca cagcgccccg ctttccgcag ggcgcgttac gcaaatactg ctttctctgg
    31261 ctcaggatta caacaactgc gccagtcggt taatatccga ctgaatcgcc ccggcggtga
    31321 cgtcgcgccc tgcacctggc ccgcggatca ccagcgggtt gtcgcgatac cagcggcttt
    31381 cgatggcaaa gacgttatcg catggcagta gtgatgccag cggatggtct tcacgcaccg
    31441 cttccacgcc cacacgcgct ttgccattgg catcgaaacg cgccacgtag cgcagcacca
    31501 gccccatttc gcgggccgct tccagtcgtt gcaccatctg ctcgttaagt tcatcgccat
    31561 tttcaaagaa atggtcgatg ctgccgcctt cgcaatgggc aggcaccagt gattcaacac
    31621 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    31681 ttacgtcttt gccggaaagg tcatcacgcg ggtcaggttc ggttaagcct tgctgccacg
    31741 cctgatccac cagttcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    31801 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    31861 gcacggtatg gttgatcggc aaacccgcac cgacggtggc gttgtacagc cagtgacgcc
    31921 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcctgcca
    31981 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcaagatac tggtcagcga
    32041 gctgctggct ggcggtaacg tccagcacca ctaaatcatc atacggatgg gcgcgcatcc
    32101 acaggaacag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    32161 ggctggcgtc cagaccgtca tagctcaaca ggctacggcg gctgtccacc acgcctgcca
    32221 gcacaaattc aaagccggta cgtgccgaaa gcgtactctg ctcacgggcg aacagttcca
    32281 gccagcggga accgatattg cccttgccga acaataccag gccgatgcgt ttttccgcgc
    32341 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    32401 ccaccaggct gatgccgtca tcggactgcc aggtaaattc gaccggctgg cctttcagtt
    32461 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac gcccgcccca accatcgcca
    32521 ccagcgccag cccctgacgc aggcgcagtt cgccaggtaa tcccgcttcg tcgaggattt
    32581 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    32641 gcacgccaac cgccagtgga cgtacctgag cacgtttcag gatctggtcg atctctttat
    32701 gcgccagttt gaaatcctgg ctggcgggca cctgaaactc aatcaagcag acatcatcat
    32761 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    32821 cttgatccgg cgtgtagcta cagcgcagtt gcaggtcgat ttcgctgcca gaaacgggct
    32881 gtaaagtacg ggcatgaaga acgggagccg ccagacgcgc cagttcgctg gcttcatcca
    32941 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggcg ctatataccc
    33001 cggcgacgtc gctccagatg gttacgcgag aaacacccgc cagcgcaccg atttgtgtcg
    33061 cggaatagtc ggaaccgtta cgccccagca gcaccgtttc accggcgttg ttacggctga
    33121 taaacccggt caccaccaga cgtttgcccg gatgttgtac cagtagctgt tgcagcaacg
    33181 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcgc
    33241 gggcatcaag ccaggccgat ggcagccctt gttgattaag caccgcagac atcaggcgcg
    33301 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccgctgt
    33361 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ttatcggctt
    33421 cttcggcggg tagcaggccg ctaatcagat cgcactgata acgacgtaat gtttgttgaa
    33481 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    33541 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagtac tccgccataa
    33601 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    33661 gttgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    33721 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    33781 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    33841 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    33901 aaacaacgac agcccgccca ggaaacgacg cagcgtctgc tcatcgccat ccagttcaaa
    33961 actcaacatt gcgccaaagc ctttttgctg gcgcgcggca atttcatgcc cctgattttc
    34021 cggcagcgac gggtgataca gtttcttcac caacggctgg gtttgcagat atttcacaat
    34081 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgcag
    34141 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    34201 ttcagtgaca acgtccgggt ctttagcaat cactacgcct gccaccacgt cagagtgacc
    34261 gttcagatat ttcgtgcatg aatgcaacac cagatcggca cctaatgcca gtggattttg
    34321 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    34381 atggcaaatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    34441 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc tttgatcaac
    34501 aaacaacacg cgatagcaac cgcgtttcgc caggctgtcg aacaggcgat agctaccgcc
    34561 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    34621 gtgaatcgcg gacatgccgg tattagtaag tacggcaccg gcaccgcctt ccagttctgc
    34681 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcacg
    34741 tggttcatta aatccggtaa agttataggt actggaaaga tggatgggag ggacaacgca
    34801 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    34861 catgtgatga agttccctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    34921 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    34981 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    35041 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccacatt aactgtgtgg
    35101 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    35161 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    35221 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    35281 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    35341 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aattccggag
    35401 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    35461 ggaaaagagc ggcgcggagt taaccagccg cttttttcat acatggacgt ttaacgatga
    35521 aaatcaggct actgatcccc ggcctgctgg tttctgtgcc tgcatttgcc tggcaaccac
    35581 aaaccggcga catcatcttt cagatctctc gctcatcgca aagtaaagcg atccaactgg
    35641 cgacccattc cgattacagc cacaccggta tgctggtgat acgcaacaaa aagccctacg
    35701 tctttgaagc cgtcggcccg gtaaaatata ccccgctgaa acagtggatc gcccacggtg
    35761 aagatagcaa gtacatcgtg cgtcgcgtta aaggcgagct aaatgccgaa caacagcaaa
    35821 aactggcgca aacggcaaaa cgttatctcg gtaaaccgta tgactccagc ttctcgtgga
    35881 gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ctaccagaat gcgctgggaa
    35941 tgcgtgtggg cgagcagcag aagctgaaag agttcgatct tagtaacccg ctagttcagg
    36001 caaagctcaa agaacgctac ggcaaaaata ttccgcttga ggaaacggta gtttcaccac
    36061 aagccgtttt cgacgcgcca caactcacca cggtcgccaa agaatggccg ctgttttcct
    36121 ggtaatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc
    36181 cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg
    36241 gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg
    36301 ttcaggtcat gaccaacggt ggagcggatt ttcattacgt taccgcaaga gcagctagca
    36361 gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc
    36421 gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata
    36481 cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat
    36541 ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct
    36601 atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg
    36661 ccagaaggca tgacggttaa agccgggagt cgcgtgcgcg tgccgtttgg caaacaacag
    36721 gagcgcatcg gggttgtggt gtcagtgagc gatgtcagtg agctgccgct taacgagctg
    36781 aaagcggtag ttgaagtgct ggacgttgag ccggtattta ctcactccgt ctggcgatta
    36841 ctgctctggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgccttg
    36901 ccgattttac tgcgccaggg gcggcctgcg gcaaacgcgc ctatgtggta ctggtttgcc
    36961 actgaacaag gtcaggcggt ggatctgaac agcctgaaac gttcgccaaa gcaacaacag
    37021 gcgctggcag cgttacggca aggcaaaatc tggcgcgatc aggttgccac gctcgaattt
    37081 aatgatgccg cgttgcaggc actgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca
    37141 ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt gcggttgaat
    37201 accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg
    37261 ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt actggaaaac
    37321 gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa
    37381 actatcgccc gttttcgtga acgttttaat gccccggtgg aagttctgca ttccggcctg
    37441 aacgacagcg agcgtctttc ggcgtggttg aaagcgaaaa atggcgaggc ggcgattgtc
    37501 atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat tgttattgat
    37561 gaagagcacg acagttccta caaacagcag gaaggctggc gctatcatgc ccgcgacctg
    37621 gcggtgtatc gcgcgcatag cgagcaaatc ccaattattc ttggctccgc aacgcccgca
    37681 ctggaaacgt tatgcaacgt gcagcagaaa aaataccgcc tgctgcgcct gacccgtcgg
    37741 gcgggcaatg cgcgcccggc aattcaacat gtgctggatt taaaaggtca gaaggtgcag
    37801 gcaggtctgg ctccggcgtt gatcacccgt atgcgccagc atttacaggc cgacaaccag
    37861 gttatcctct ttcttaaccg ccggggtttt gcgcctgcgc tactgtgcca cgactgtggc
    37921 tggattgccg aatgcccgcg ttgcgatcac tactacacgc tgcatcaggc acaacagcat
    37981 ttgcgctgtc accactgtga cagccaacgc ccggtgccgc gccagtgccc ttcctgcggt
    38041 tccacacacc tggtacccgt ggggctgggc accgaacagc ttgaacagac gctcgcgccg
    38101 ctgttccccg gcgtgcccat ttctcgtatc gaccgcgata ccaccagccg caaaggggcg
    38161 ctggaacagc aactggcgga agtgcatcgc ggcggcgcga ggatcttgat tggcacgcaa
    38221 atgctggcga aaggtcacca ctttccggat gtgacgctgg tagcgttact ggatgtggac
    38281 ggcgcgctgt tttctgccga ttttcgctcg gcagagcgtt tcgctcagct ttacacccag
    38341 gtcgctggtc gtgccgggcg tgcgggtaaa cagggcgaag tggtgctgca aacgcaccat
    38401 ccggaacatc ctctgttgca aacgttgctc tataaaggct acgacgactt tgccgaacag
    38461 gcgctggctg agcgacggat gatgcagctg ccgccatgga ccagccatgt gattgtgcgt
    38521 gcggaagatc ataacaatca gcacgcgccg ctgttcctgc aacagttgcg taatctgatc
    38581 ctcgccagcc cgcttgcaga cgacaaactg tgggttctcg gtccggttcc ggcactggcc
    38641 cctaagcgtg gtggccgctg gcgctggcaa attttgttgc agcacccttc ccgcgtgcga
    38701 ttacagcaca tcatcaatgg tacgctggcg cttattaata caataccgga ttcccgtaaa
    38761 gtgaaatggg tgctggatgt cgatcccatc gagggctaaa ccgctcacga tgcgaggcgg
    38821 atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc
    38881 tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca
    38941 ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg
    39001 ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat cccgataaag
    39061 tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc
    39121 agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg attgtcccgg
    39181 atatctgcga ccctttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc
    39241 acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta
    39301 tcgatttgat catcaccaag caaattgatg gcatgttgtt gctgggttca aggctgccgt
    39361 ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg
    39421 caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca tttgatgcag
    39481 taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt cccgaagaga
    39541 tgccgctgtg tcactaccgc ctgcaaggct atgttcaggc gctgcgtcgc tgcggcatta
    39601 tggttgatcc gcaatacatc gcccgtggcg acttcacctt cgaagccgga agcagagcaa
    39661 tgcaacagct gcttgatctt ccacaaccgc ctactgctgt cttctgccat agcgatgtga
    39721 tggcgctcgg cgcactttct caggcaaaac gtcaggggct gaaagtcccg gaagaccttt
    39781 ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca
    39841 tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc
    39901 aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat
    39961 caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc
    40021 cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa
    40081 cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg
    40141 aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt
    40201 cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga agagtccgag
    40261 acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga agaacgctgg
    40321 cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct
    40381 gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgcca gcttcttgaa
    40441 caaatgcagg ccgatatgcg ccagcagcca acgcagctgg ttgaagttcc gtggaatgag
    40501 cagacgccgg aacagcgtca gcaaacgcta cagcgccagc gtcaggcgca gcaattagcg
    40561 gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg
    40621 cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc ggcttctact
    40681 cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg
    40741 cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa
    40801 gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg
    40861 gtacgtgctc agctggcgtt cgaaggcttt gactcaaaaa ttaccaccaa caatggctgg
    40921 aatcgtgtgg tgattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgc
    40981 ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa
    41041 aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt
    41101 attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg
    41161 tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga
    41221 aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg
    41281 atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag ggccatctgg
    41341 tcaaagccgc cgttgagctg gcgaaagact ggcgtaccga tcgcatgctg cgcaaacttg
    41401 aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
    41461 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
    41521 cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt
    41581 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
    41641 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
    41701 tggataagca catcatcggc caggacaacg ccaagcgttc cgtggcgatt gctctgcgta
    41761 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
    41821 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc
    41881 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt caccgaagtg ggctatgtcg
    41941 gtaaggaagt ggattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
    42001 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
    42061 acgtactgat cccaccggct aaaaataact ggggacagac cgaacagcag caggagccgt
    42121 ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa
    42181 ttgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg
    42241 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggtcag aagcaaaagg
    42301 cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
    42361 tggtgaaccc ggaagagctg aagcaagatg ctatcgacgc tgttgagcag cacgggatcg
    42421 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
    42481 ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcact gtttccacca
    42541 aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
    42601 cgaaaccgtc tgacctgatc ccggaactgc aaggtcgtct gccaatccgc gttgagctgc
    42661 aggcactgac caccagcgac tttgagcgta ttttgaccga gccgaatgcc tctatcaccg
    42721 tgcagtacaa agcactgatg gcaactgagg gcgtaaatat cgagtttacc gactccggta
    42781 ttaagcgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
    42841 gtcgtttaca taccgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
    42901 taagcggtca aactatcact attgacgcag attatgtgag caaacatctg gatgcgttgg
    42961 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
    43021 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
    43081 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
    43141 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatcca ctggtcgccc
    43201 tgctggcatt gattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
    43261 gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa
    43321 aaggggtcat tacccagcaa gagatgaaac gggcgctcat tattaccgtc gtgctcatct
    43381 gtctctccgg gctggcactg gttgcagtgg cgtgccatac gctggccgat tttgtcggtt
    43441 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
    43501 cttatggtta tatcggtttg ggtgatattt ccgtactggt tttctttggc tggttgagcg
    43561 tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc cttccggcaa
    43621 ccgcatgcgg cctgctggca acggcagtac tgaacattaa taacctgcgt gatatcaata
    43681 gcgaccgcga aaacggcaaa aacacgctgg tggtgcgctt aggtgcagta aacgcgcgtc
    43741 gttatcatgc ctgcctgctg atgggctcac tggtgtgtct agcgctgttt aatctctttt
    43801 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accgttgctg gtgaagcaag
    43861 ctcgttatgt gatgcgcgaa atggacccgg tggcgatgcg accaatgctg gaacgcactg
    43921 tcaaaggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
    43981 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
    44041 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
    44101 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
    44161 cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
    44221 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggagg
    44281 ttctgttcgc cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
    44341 ggaaggcctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat
    44401 cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag
    44461 cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
    44521 caataccggg attattcttt cagaagatcc gctggatatt gaatgataaa aaaggcaccg
    44581 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga
    44641 tgaagtgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
    44701 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
    44761 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
    44821 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
    44881 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aatcctcgtc
    44941 ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga
    45001 agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat
    45061 ccttctcctg gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat
    45121 gatctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt
    45181 ttatgacgga gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa
    45241 gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat
    45301 taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg
    45361 aattcctcgg taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag
    45421 tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa
    45481 tggccatcta cctgaccgca ggggtttccg gtgcgcatct taatcccgct gttaccattg
    45541 cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag
    45601 ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg
    45661 acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggctggca
    45721 ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg
    45781 tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac
    45841 cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta
    45901 tgggcccatt gacgggcttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg
    45961 cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc
    46021 tggtgccgct ttttggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac
    46081 tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc
    46141 cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa
    46201 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    46261 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc
    46321 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    46381 ctggcgaaag ctgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    46441 gaaaccacta ttgtctggga aaaagaaacc ggcaaaccta tctataacgc cattgtctgg
    46501 cagtgccgtc gtaccgccga gatctgcgaa catctcaaac gcgatggcat ggaagagtac
    46561 attcgtaaca acaccggtct ggtgattgac ccgtacttct ctggcaccaa agtgaagtgg
    46621 atcctcgacc atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt
    46681 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    46741 accaacgcct ctcgtaccat gttgtttaac atccatactc tggactggga cgacaaaatg
    46801 ctggaggtgc tggatattcc acgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    46861 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    46921 ggtgaccagc aggcggcgct gtttggtcag ttgtgcgtga aagaaggaat ggcgaagaac
    46981 acctacggta ccggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa
    47041 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtaaacta tgcgctggaa
    47101 ggtgcggtgt ttatggcggg cgcgtccatt cagtggctgc gcgatgagat gaagttgatt
    47161 aacgacgcct acgattccga atattttgcc accaaagtgc aaaacaccaa tggtgtgtat
    47221 gtggttccgg cctttaccgg gctgggtgcg ccatactggg atccgtatgc acgcggggcg
    47281 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    47341 attgcttatc agacgcgtga cgtgctggaa gcaatgcagg ccgactccgg tattcgtctg
    47401 cacgcgctgc gcgtggacgg cggtgcggtg gcgaacaatt tcctgatgca gttccagtcc
    47461 gatattctcg gtacgcgcgt tgagcgcccg gaagtgcgcg aagtcaccgc tttgggtgcg
    47521 gcctatcttg ctggtctggc ggttggcttc tggcagaatc ttgacgagct gcaagagaaa
    47581 gcggtgattg aacgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac
    47641 gcaggctgga aaaaagcggt caaacgcgcg atggcatggg aagaacacga cgagtaataa
    47701 tgattgcctg atgcgacgct tgcgcgtctt atcaggccta cgagtcggat acacatccgg
    47761 catcacgcag actctgaaac cgtgcggggg caaccccgca cacatcaata atcccgccct
    47821 tcccctgtgc tacacttcgc gccattcctt actgcttaga gtttgctatg agacgagaac
    47881 ttgccatcga attttcccgc gtcaccgaat cagcggcgct ggctggctac aaatggttag
    47941 gacgcggcga taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt attatgctca
    48001 accaggtcaa cattgacggc accatcgtca ttggtgaagg tgaaatcgac gaagcaccga
    48061 tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat attgctgttg
    48121 atccgattga aggcacgcgc atgacggcga tgggccaggc taacgcgctg gcggtgctgg
    48181 cagtaggcga taaaggctgt ttcctcaacg cgccagatat gtatatggag aagctgatcg
    48241 tcggaccggg agccaaaggc accattgatc tgaacctgcc gctggcggat aacctgcgca
    48301 atgttgcggc ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt ctggcaaaac
    48361 cgcgccacga tgccgttatc gctgaaatgc aacaactcgg cgtacgcgta tttgctattc
    48421 cggacggcga cgttgcagcc tcaattctca cctgtatgcc ggacagtgaa gtcgatgtgc
    48481 tgtacggcat tggcggcgcg ccggaaggcg tggtttctgc ggcggtgatc cgcgcattag
    48541 atggcgacat gaatggtcgt ctgctggcac gtcatgacgt caaaggcgac aacgaagaga
    48601 atcgtcgcat tggcgagcag gagctggcac gctgcaaagc gatgggcatc gaagccggta
    48661 aagtattgcg tttgggcgat atggcgcgca gcgacaacgt catcttctct gccaccggta
    48721 ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg caatatcgcc accaccgaaa
    48781 cgctgctgat ccgtggtaaa tcacgcacca ttcgtcgcat tcagtccatc cactatctgg
    48841 atcgtaaaga cccggaaatg caggtgcaca ttctctgagt gatttgatcg attgagcctt
    48901 ccagtccttc gggactggaa tttttttgtt cggagaacga agataaggca agtcaatcaa
    48961 aacaggagaa aaacatggct gattgggtaa caggcaaagt cactaaagtg cagaactgga
    49021 ccgacgccct gtttagtctc accgttcacg ctcccgtgct tccgtttacc gccgggcaat
    49081 tcaccaaact tggccttgaa attgaaggcg aacgcgttca gcgtgcctac tcttacgtta
    49141 actcgcccga caatcccgat ctggagtttt acctggtcac cgtccccgat ggcaaattaa
    49201 gcccgcgact ggcggcgcta aaaccgggtg atgaagtgca agtggttagt gaggcggcag
    49261 gcttctttgt gctggatgaa gtccctgact gcgaaacact gtggatgctg gcgactggta
    49321 cggcgattgg cccttattta tcgattctgc aattgggcaa agatttagaa cgcttcaaaa
    49381 acatggtgct ggttcacgcc gcacgatatg ccgccgactt aagctatcta cctctgatgc
    49441 aggaactgga aaaacgctac gaggggaaac tgcgcattca gacggtggtc agtcgggaaa
    49501 cggctgcggg ttcgctcact gggcggatac cggcgttaat tgaaagtggt gaactggaaa
    49561 gcgcggttgg cctgccgatg aataaagaaa ccagccatgt gatgctgtgc ggcaatccac
    49621 agatggtgcg cgatacacaa cagttgctga aagagacccg gcagatgacg aaacatttac
    49681 gtcgccgacc gggccatatg acagcggagc attactggta agcggttact tgtcgataaa
    49741 cggcacgatc aagagatccg cactcaactt attgatcatc ccgcgatatg ccggcatcaa
    49801 acggttgata aatgagtggt gatgaccaca gacaaggagg tcgcactgct ctttttgcat
    49861 aatttccagc agtgtttccg gcatttctcc gcgttcaata cgcagttttg tcttcggcca
    49921 ttgaatattt ttcgtcagtt tatacagctt gttatccgac ttattcttca acaattgaag
    49981 aatatcttct gttgcaggga agtagatacc cggatacaac tcgcttaagc catcatcaat
    50041 atgaattaac gtcaggtgag cgtcattatg tctggcgagc tccagggctt tattcaccag
    50101 taaggcatct tcttcattcc cggaaattgc cacgccaatg tgtttataag ccatgtttaa
    50161 ctccttctaa agcaactcca tcaagctaac gaacgcagtg ataactcaaa aataatcatc
    50221 tcggcctggc atgagaaggt gaaggccgca tcaagctcaa cttgcccttc cagatcttta
    50281 atcttatagg ccacgctggc cccttcatct gcagaggatg cagcggcggc aagctctttc
    50341 agtttggtca taaaggactc tgcttcttcg cgggtggcaa aaaagcgcga aaacttactg
    50401 gtacagttgt cgttatcaat caccgtaccg atatcaatcg cacatccttt agtactgcat
    50461 ttatctacga catctttcat agggcacctc tgtatattcg cctctctgtc gattacgact
    50521 taagaataat gcgccgttaa aggaagaaca aacaagtaat gatcttatgg attaccattt
    50581 tttttgatat tgcttaaggt gaagagttca gaaggatttt ccgggcattg tcacttgcat
    50641 cgtggaaata acaatcccgg aaaaaaatag cgataagaga tatattaaag gttaattact
    50701 tgatttattg tcggctttat acttcacatc ctgggtatct ttaccatatt tattttcgcc
    50761 ttgtgtgcca acaaacgcac caagatcgat aagcatcatc accagaatca gtgttgggat
    50821 aaaacgcccc accgcccatt gccatacccc cggcaaaatt gcccagttac ccgccagcag
    50881 catccacgcc acaatcatca gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg
    50941 cttaacggtt actgccgctg ttggccaaag caagcacaca aggcaaaacg ccgcggtctg
    51001 aatatcgagt aaatttttac ccgccagtga gaaaagcacc agcatgcctg cgaaccacag
    51061 gcctatccaa atccagaaat cacggcgtcc aatacgccct ttaaatgaga ataaccattg
    51121 ctgtatggtc atgtaagttc cttgatggtt gtcttttcca ggattctacc cttttgacaa
    51181 gggcgacaga tatcgtttta atcggagcca gtcataacaa aaggtactgt caatgaagcc
    51241 agggtgtacg ctgtttattc tcttatgttc tgcattaacc gttacaacaa cggcgcatgc
    51301 acaaacacca gatacggcaa cgaccgcgcc ttatctgctg gctggagccc ctactttcga
    51361 tctctccatc agccagtttc gagaagactt taacagccag aatcccagcc tgccactgaa
    51421 cgaatttcgt gccatcgaca gcagtcccga caaagccaat ctcactcgtg cagcaagtaa
    51481 aattaacgag aacttgtatg cttctactgc gctggagcgc ggtaccttaa aaatcaaaag
    51541 cattcaaata acctggctac ccatccaggg gccagagcaa aaagccgcga aagcgaaagc
    51601 tcaggcatac atggcagcgg tgatccgcac actcacccca ttaatgacca aaactcaaag
    51661 ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt acaccgagac
    51721 agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt
    51781 tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc
    51841 aaagcctttg tgccgatgaa tctctatact gtttcacaga cctgctgccc tgcggggcgg
    51901 ccatcttcct ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg
    51961 ggtaactgga aactgaacgg cagccgccac atggttcacg agctggtttc taacctgcgt
    52021 aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg caccaccgga aatgtacatc
    52081 gatatggcga agcgtgaagc tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac
    52141 ctgaacctgt ccggcgcatt caccggtgaa acctctgctg ctatgctgaa agacatcggc
    52201 gcacagtaca tcatcatcgg ccactctgaa cgtcgtactt accacaaaga atctgacgaa
    52261 ctgatcgcga aaaaattcgc ggtgctgaaa gagcagggtc taactccggt tctgtgcatc
    52321 ggtgaaaccg aagcggaaaa cgaagcgggc aaaactgaag aagtttgtgc acgtcagatc
    52381 gacgcggtac tgaaaaccca gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa
    52441 cctgtatggg caatcggtac tggcaaatct gcaactccgg ctcaggcaca ggctgttcac
    52501 aaattcatcc gtgaccacat tgctaaagtt gacgctaaca tcgctgaaca agtgatcatt
    52561 cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca gccagacatc
    52621 gacggcgcgc tggttggcgg tgcttctctg aaagctgacg ctttcgccgt gatcgttaaa
    52681 gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc cggatttcat atccggcact
    52741 tactttccta aactcctcgc cttaacgcaa aatctcacac tgatgatcct ggatttcttc
    52801 ggctgaagca cggttcagcg tcagtagatt tcgttgtgtc gccagcaata caaatgagtt
    52861 atcactctgc cgtaccatcg ccagcccgta gcttcccata tgttcccgcg cctcaggcac
    52921 ttcttccgcc agcatcataa atgggctgcg ttgtaccagt tcgctttccg ttacccgacg
    52981 cgccaggtac tcatgcccac gcaaaccacc tggcagtggc aaccagcggc tactgatgtt
    53041 cgccagattg ttatccagct gttcgcgcac atcaggacga atacaagaaa tatgaatatg
    53101 aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc agagataccg cgcgatcggg
    53161 aacaggctgg ccgtattttt ggctcataaa atcacgcgcc tgccaggcta accagaaaaa
    53221 attaggcgtt gacggatcgg tcaacaatgg gctttcagta ccgttaatac ggtacgttgg
    53281 cattaacaga tattgcagtg ggccatgacg atcttttaaa accacgtatc cggcgttggg
    53341 tttgacttcc gcacatggcg acgggttttg gttttcctgc tggttgggca aacattgctc
    53401 aagaactatc ttacgtaacg tgtctgactc ttcaccggtt aatttccagt aaccaatacc
    53461 ggcagtcaca acggcgataa ctatcatcac caaaaaaaga agacccgctt ttttcatctt
    53521 tttttccctg tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc
    53581 cagtggcgag aaaccaccgg gcttgataat tatcctgggg aaatcagcgt ttgctgatct
    53641 gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact
    53701 cttcatcaat ggtgaataac ttcaattttg gaaacgcatt ttcgtacttt ttcgctacct
    53761 cagcgtcgcg cggacggtag tagtttttcg ctgcaatttc ctgaccttct ggcgagtaga
    53821 gatatttcag gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga
    53881 ccaccgacac ggttggctct gcgaggatag actcactcgg cgtgacgatt tcgaatttat
    53941 ctttccccag ttcattcgct gccagcagag cttcgttttc ccaggcaatc agtacatcgc
    54001 caattccgcg ctcgacaaaa gtgttagtgg atccacgcgc gccagaatcc agaacttcga
    54061 cgtttttata cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat
    54121 gaagcgcgta gccccaggct gccaggtaat tccagcgcgc gccaccagag cttttcggat
    54181 taggcgtgat caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg
    54241 gattaccctt acgtaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca
    54301 gacgtttgat ccactcttta tcaatccgcc cgcgttccgc aattgcgtcc acgtcatagg
    54361 ccagagccag cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttgc
    54421 ctgaaccacc gtgtgactga cgaatcacca cgttatcgcc agtttgctgt ttccagtggg
    54481 cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa
    54541 gaagctgaat atcctttgcc ataacgctgg ttgccaccag caaaaatgtt aaccctacgc
    54601 cccacttttt catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa
    54661 ggttgaccaa acattaaaga ataaaaaaag attggctata acttgcgagt atatgttggg
    54721 ggattaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc
    54781 cggatgcggc acaaacgcct tatccggcct acaaaagttt gcaaattcaa tagattacag
    54841 aattcatgta ggcctgataa gcgaagcgca tcaggccttt ttgcttctgt catcggtttc
    54901 aggctaaagg aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc
    54961 cagtcgcctt tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc
    55021 agctgttcgt tctggatacc tacgcaacga ccaccgtaac ctgccagcag cagatcgata
    55081 gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc
    55141 tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc
    55201 gccagttcgt caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct
    55261 ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga
    55321 accacaacga attcacagcc accggcaatg gccgcagcca acgtcagatc gccacaataa
    55381 cggcccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg
    55441 tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct
    55501 ttgatgtcgt tgtcgatagt gcccggcagg ccgatgcacg ggaagcccat ttcggtcaga
    55561 cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gatcccacgt
    55621 tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc
    55681 gcagaaccga ggaacgtacc gccacggttg atcatgtcag aaacgctgta acggtctagc
    55741 tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatgcccat tacttccaga
    55801 ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca
    55861 ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact ttggaatgca
    55921 aaatgaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa
    55981 tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat
    56041 acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc
    56101 tccctgggta cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc
    56161 cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag
    56221 tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga
    56281 tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttcc
    56341 tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg
    56401 ctatataaaa tatagatgcc gatccccaat gcaaacagag catcggcgcg atgccagccg
    56461 taccaggaca accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa
    56521 tgtagcatat cagcccgcac cgcctggctt tgcgtccggc gcaccaccca acgctgaaac
    56581 gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga
    56641 tctgtcatcg gtgttggaga taccagatgt tgaatacccg tcaaaaacag gaatagtgcc
    56701 gaaccggaga taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca
    56761 aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac
    56821 gacgcaccga tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta
    56881 taccaccatg caaaaatttt aatcagcaat agcagcgaag ccatcgccgt cgcagcaatc
    56941 gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga
    57001 cggtagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata
    57061 aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag
    57121 ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg
    57181 ccattgcgtc acgtcgcgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc
    57241 ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg
    57301 acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa
    57361 attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg
    57421 ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact
    57481 tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca
    57541 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc
    57601 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    57661 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    57721 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    57781 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    57841 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    57901 acgacagcat tgatttactt ttgcttgatg taatgatgcc gaagaaaaat ggtatcgaca
    57961 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    58021 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    58081 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    58141 aacaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttaagtctca
    58201 atcctggtcg ccaggaagcc accttcgacg ggcaaacgct ggagttaacc ggtacggagt
    58261 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gagcatttaa
    58321 gccaggaagt gctgggcaag cgcctgacgc ctttcgaccg cgctatcgat atgcacattt
    58381 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc
    58441 gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    58501 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    58561 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcagggtc tgatgattga
    58621 gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    58681 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttac tggtgaccac
    58741 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    58801 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    58861 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    58921 cagttctcaa tccgatttca taaacttact gtttgaccgc ccgctattac tgctgattgt
    58981 caccatgctg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    59041 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    59101 actggaagcg gggccacagg aattccttgc cgccggtgcc agttttaacc agatggtcac
    59161 cgcgctggag cgcatgatga cctctcagca gcgtctactt tctgatatct ctcacgaact
    59221 gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga
    59281 aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga
    59341 tctgttggtg atgtcacgta atcagcagaa aaatgcgctg gttagcgaga ccatcaaagc
    59401 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    59461 gtcgttgacg gttaacttcc cacctgggcc gtggccgctg tacggcaacc cgaacgccct
    59521 ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga
    59581 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    59641 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg
    59701 cgatcgtgaa tctggcggta caggtttggg gctggcgatt gttgaaaccg ccattcagca
    59761 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    59821 gttgccgctg tataagcggt cataacgtca accttcaggt aaaaaaatgc ctgatgcact
    59881 acgcttatca ggcctacaaa acctgttgaa tttatgggtt ttgtaggctg gataaggcgt
    59941 ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc gtcttatcag
    60001 gcctacactc cggggttatt ttccccacaa acggcgggaa aaatcttcga tcttgccgct
    60061 cagtcggcgc ttttgcatcg ttcgcgtcca gcttttcgat aaccctgcaa cggagagtaa
    60121 acggtgatac tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac
    60181 ggtcacatcg ctgacacggg aaaccaactc cagtggcgca tcagcagata ccaaccctgg
    60241 tgcaattacg ctatacaacc accccacttt acccgtgttt tgcatcaact gcgctatatc
    60301 gctgatatca aaatggtaat tgagtttata gcaaggtgag cgtggctggc tgacctgaat
    60361 taatgcctct ccccagcgga aaatatcacc aatataaaca ttactttccg tcagaccgtc
    60421 ggttgagaga ttttcgccaa acgcaggcgc aacaaacagc tccgcctgtt ccggaaattc
    60481 ccgcgcccag tagagataat gctcacgagg ataatgacac agcgctctgt ctggcccgcc
    60541 gtggatcttt ttctccgcct gttcgtcacc ttccagcccc aggtccgtca gcatcaactc
    60601 accatcaacc tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt
    60661 gcctgtgtat acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat
    60721 tagaccatat ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcagaaac
    60781 gcaattgaaa accggacgaa cgttagttca tccggctgtg gggattaagg aagctggttt
    60841 atttcaccgt acctaaaatt tcgattttcg ccgctctggc tttgacttta cgtgaccaga
    60901 tagaggtaag aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag
    60961 ttgctgccgg agccataggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg
    61021 ctaccgcggc cccagcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa
    61081 tcaatttatc ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca
    61141 ggatccccag caaacctgtc tgggcaatta cagtcaaatc aatggtattg cccagtgcga
    61201 aggcaaagaa tggaatcagc gtctgcaccg ctttgctgaa aaattctcgt aactcagggt
    61261 caaggttccc cagggcaaag cccaccagga acggcaatac tgcgccgacg aaaacatgcg
    61321 gttcaaacga ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact
    61381 ccaacgacat caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg
    61441 aagcgtaaag tccgccgttg gtcatatcca tcgccgccac cagcgccaac gttgaaagtc
    61501 cggcaaaaaa tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa
    61561 ccacccacgc gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc
    61621 ccgtcgcgct taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca
    61681 ccgtaccggt aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga
    61741 aggtgtggca cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct
    61801 caatcgagcg ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg
    61861 tcattcctca acggagcatc agtgttgatg gaaacgaaga taaacattca tttgatcaat
    61921 aaaagtgatc aaaaccacat caattgaaac gatgttttat ttttatcgga tcgttgtttt
    61981 tattttgaaa gtgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg
    62041 caatgcggcg taaacgcctc attgcagcag gcgtcaaatg attatttttt cgccgcgaaa
    62101 cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg
    62161 cggttctgga atttcaggta gtaagcatgt tcccacacat ccaggcccac aatcgggaag
    62221 ccggaagcgc cagaaatggc ttcgcccatc agcggagagt cctggttagc agtagaaacc
    62281 accgccagtt tatcgccttt ttgcaccagc catgcccagc cggaaccaaa gcgggaagct
    62341 gccgcttttt caaattctgc tttgaaatta tcaacggaac cgaaatcacg ttcgatagcc
    62401 gctttcaggt cgccctgcag ggtggtgcct tttttcagac ctttccagaa cagactgtgg
    62461 ttagcgtgac cgccagcgtt attgcgcagt acggttttct tgtctgctgg cagctggtcc
    62521 agtttggtga tcagctcttc aaccggcagg ttagcgaatt ctggcaggct ttccagcgcc
    62581 gcattggcgt tgttaacgta ggcctgatgg tgtttggtgt ggtggatttc catggtctgc
    62641 ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtataactc
    62701 atattcatct ccagtgtggt cgggcggccg attgttaatg ccgcgtaagc agttggttca
    62761 ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc ataagggtat
    62821 ggttttgcag gaaaatgccc aagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
    62881 agtaaatcgg cagcggcggc aacaggcgaa aggttaatcg acagctcaat ttttacactc
    62941 atctcgtcgg agatgtgacg cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat
    63001 tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgcgcc
    63061 gttcaaaaaa gtaaaaaagt ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc
    63121 gtggattatc ctgccgtggg ccatcagcgc cctgttacta ccgaatttct gggcgtatta
    63181 cagctcattt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat
    63241 cggtaatatc aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat
    63301 cgccattggc attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt
    63361 cgatgtgctg attaataccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct
    63421 gattggcgta gggattgtaa ctcgcgccgg gcagttgaaa gagcgcaaga tgggcattaa
    63481 agccgaagag ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc
    63541 tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcgct
    63601 gggcgtcgat cccctgtatg tcgctttgcc aagctatgtt gtcatcatgg gcggcggcgc
    63661 aatcatcaac ctcggcttct gcttcattcg tctggccaaa gtgaaagatt tgtcgctaaa
    63721 agccgacttc tcgctggcaa aaccgctgat cattcacaat gtgttactct cagcactggg
    63781 cgggttgatg tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc
    63841 gcagtatgac tacatcagct ggatgctgca tatgagcttc tatgtattgt gcggcggtat
    63901 cgtcgggctg gtgctgaaag agtggaacaa tgcaggacgc cgtccggtaa cggtgttgag
    63961 cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta
    64021 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
    64081 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
    64141 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgcc gcagatattg
    64201 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacacgct cactgcatga
    64261 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
    64321 aatcagctta tccagcaatg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
    64381 atggcgattc agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa
    64441 cgacacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
    64501 ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc
    64561 gccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac
    64621 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc
    64681 acgggtaatg cgataagggc gatcgttgag tacatgcaga ccattaccgc gccagacaat
    64741 caccagctca caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc
    64801 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
    64861 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
    64921 tacgttgaga agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg
    64981 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc
    65041 agcgataggc gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca
    65101 tcagtcgcag gcggttcagg taacgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
    65161 gatgtagcgt acgcaatgaa agagaaaatt gagccgccac tgcatcccaa ttcacctcat
    65221 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
    65281 cgctctcctg caaactgctt ttacgcagta agagcagtaa ttgcataaac agaatttcac
    65341 gactggcggt cgagggtaaa tcatttccct cttcttgttg ttccaactgt gcaaccagtt
    65401 ggcgcacctg ctgtaatacg caatggttaa cgcgccagtg agacggatac tgcccatcct
    65461 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
    65521 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
    65581 gtacgaaaca aaccgtccca ccggtgatgg tatagggctg cccattaaat acatgaatac
    65641 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
    65701 cctgcgggag ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac
    65761 tatgtaatac ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga
    65821 ggtcaggttc ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa
    65881 gatacagcgt gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagcaaatt
    65941 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
    66001 gagaaggtca cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac
    66061 attatgacct ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg
    66121 ctggcgcgtt acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac
    66181 aatggcctgc atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc
    66241 attcgccttg gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt
    66301 gatacctggg gcgtggactt tgtgctgctc gaccagcacg gtcagcgcgt gggtctgccc
    66361 gttgcttacc gcgatagccg caccaatggc ctaatggcgc aggcacaaca gcaactcggc
    66421 aaacgcgata tttatcaacg tagcggcatc cagtttttgc ccttcaatac gatctatcag
    66481 ctacgtgcac tgacagagca gcaaccagaa cttattccac acattgctca cgctttgctg
    66541 ataccggatt actttagtta tcgcctgacc ggcaagatga actgggaata taccaacgcc
    66601 acgaccacgc aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg
    66661 agcggtgcca acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cataggtcac
    66721 tggatttgcc cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc
    66781 agcgcggtta tcgcctcgcc gttaaacggt tcacgcgccg cttatctctc ttctggcacc
    66841 tggtcattga tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc
    66901 aatatcacca acgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc
    66961 ttatggctgc ttcagcgagt gcttcaggag cggcaaatca acgatcttcc ggcgcttatc
    67021 gccgcaacac aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt
    67081 attaatcctg acgagatgtg cagcgaaatt caggctgcgt gtcgggaaac ggcgcaaccg
    67141 atcccggaaa gtgatgctga actggcgcgc tgtattttcg acagtctggc gctgctgtat
    67201 gccgatgtgt tgcatgagct ggcgcagctg cgtggtgaag atttctcgca actgcatatt
    67261 gtcggcggcg gctgccagaa cacgctgctc aaccagctgt gtgccgatgc ctgcggtatt
    67321 cgggtgatcg ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg
    67381 acgctggatg aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac taccgcgaat
    67441 ctgaccacct ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagattcac
    67501 tctacacgac agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc
    67561 ttgcgaaaca gcgtttcgcg gcagtaggga ttgatgtcga ggaggcactg cgccaacttg
    67621 atcgtttacc cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc
    67681 cggaaggttc gctgaccggt ggtattcagg ctactggtaa ttatccgggc aaagcgcgta
    67741 atgccagcga gctgcgtgcc gatctggaac aggctatgcg gctgattccg gggccaaaac
    67801 ggcttaattt gcatgccatt tatctggagt cagacacgcc agtctcacgt gaccaaatca
    67861 aaccagagca cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt
    67921 ttaacccctc ctgtttttca catccgctaa gcgccgatgg ctttacactt tcccatgccg
    67981 acgaccgcat tcgccagttc tggattgacc actgtaaagc cagccgccgt gtttcagcct
    68041 attttggtga gcaactcggc acaccgtcgg tgatgaacat ctggatcccg gatggcatga
    68101 aagatatcac cgttgaccgt ctcgccccgc gtcagcgttt gctggcagca ctggatgaag
    68161 tgatcagcga gaagctaaac cctgcgcacc atatcgacgc cgttgagagc aaattgtttg
    68221 gcattggcgc ggagagctac acggttggct ccaatgagtt ttacctgggg tatgccacca
    68281 gccgccagac ggcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg
    68341 acaagatttc cgccgccatg ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg
    68401 ttcgctggga cagcgatcac gtagtgctgc tggatgatga aacccaggcg attgccagtg
    68461 agattgttcg tcacgatctg ctcgaccggg tgcatatcgg cctcgacttc ttcgatgcct
    68521 ctatcaaccg cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc
    68581 gtgcgttgct ggaacctacc gctgagctac gcaagctgga agcggcgggc gattacactg
    68641 cgcgtctggc gctactggaa gagcagaaat cgttgccgtg gcaggcggta tgggaaatgt
    68701 attgtcagcg tcacgatacg ccagcgggta gcgaatggct ggagaacgtg cggacttatg
    68761 agaaaaaaat tttgagtcgc cgcgggtaaa cactgccgga tgcggcacaa gtgccttatc
    68821 cggcctacgg gtcggcaata gttgtaggcc tgataagacg cgacagcgtc gcatcaggca
    68881 ttgattgccg gatgcggcac gagtgcctta tccggcctac gggtcagcaa cagttgtagg
    68941 cctgataaga cgcgacagcg tcgcatcagg cattgattgc cggatgtggc gcgagtgcct
    69001 tatccggcct acaggtcgtg catccgacaa caccgaatta acaggaacac agaacatgca
    69061 aaacataacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct
    69121 gaaaggctgg gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat
    69181 cgcaccatat cacgacaatt ttcaccaaca accgcgctat atcccgctca gccagcccat
    69241 gcctttactg gcaaatacac cgtttattgt caccggctcc ggcaaattct tccgtaacgt
    69301 ccagcttgat cctgcggcta acttaggcat cgtaaaagtc gacagcgacg gcgcgggcta
    69361 ccacattctt tgggggttaa ccaacgaagc cgttcccact tctgaacttc cggctcactt
    69421 tctctcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg
    69481 ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg cggtcttcac
    69541 tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat
    69601 tctgccgtgg atggtgcccg gcacggacga aatcggccag gcaactgcac aagagatgca
    69661 aaaacattcg ctggtgttgt ggcccttcca cggtgtgttc ggcagcgggc cgacgctgga
    69721 tgaaaccttc ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta
    69781 ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc tcggccagcg
    69841 tttcggcgtt acaccactcg ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa
    69901 agcctttgtc atgcaggtaa accccgacgc ccacgaagag tatcagcgtc ggcataatcc
    69961 catctggccg gaactggaag cagtgctgaa atctcacggt gcgcataact acgccatcta
    70021 tctcgacaaa gcgcgtaatc tgctatttgc cacgatagag attgaatctg aagagcggtg
    70081 gaatgcggtt gccagtacag atgtttgcca acgttggtgg aaatatatga ccgacgttat
    70141 gcccgctaat gcggataaca gcccagtcag tagcgaactg caagaagtgt tttacctgcc
    70201 ttaatgagtt gcggtgccgg aaaatatcca gcaccgccct ttcatcagaa gaagtattta
    70261 aagcgaacgc gtgcgccgta acggtcatca ctatcgtcgc cgtgtatctt atcgccgtcg
    70321 ctatccagaa tggagtaata cgtgccgagg taaatattaa agttctccat atccatcaca
    70381 ttagcgaact gataagacgc atgaatggtg tgaatgttgt atgttccttc atcatcaatc
    70441 cagcaatcgt tatcacaaac cacgccacta aattcatcaa tcttattatg tgcgtagata
    70501 taacccagct cgaaacgttt ccacagcgcg ttaatcccag cggtgaaatc tttctcatta
    70561 ttagcatcta aataggcggt attaagatta accacgatgc cattttccgg atcggttttc
    70621 aggccattcc aggtcatgct catgccataa ccggtacgat cggactgatc gacaaaatta
    70681 cccttgctat cggtataacc ataagcatta tttaccacgt tcgcttccat cgctgcggaa
    70741 acggtgaatt cttccgtcgg cgaccaggca attaccggac gcagataagc aacatttttc
    70801 tgctgttcca tatcgcgtcc atgataatta ccgtcgttat ataaagatgt tccgtcttcc
    70861 agtaacgtgt ttaattcaaa ataccagtta tcgagttgtt tgctgacgag gaaattaccg
    70921 ccagcgttag aacgcccgcg gccctctttc atcatataga tataaccgct gccatcgtca
    70981 taaagatcgt tcgcagtatt accggaatgt tcaacaaagg tatcctgatt cagcgggaac
    71041 atatcgtagg cttcaaaacg gcctactttg accttccagt cgttttcttt gccaaagaag
    71101 aaaaccgcat catcgatatt tactgaaccg tgcatatccc ccagcggttg tgcggagaac
    71161 ccagcgaaat agccattatc cagttttcgc ataccatcaa aacctaacag aatacgacca
    71221 ttgagattcc actgttcatt agtgggatcg ctattcacat cagcgttggt cattgccagc
    71281 aggccatgat tactttccgc atccatatta aattctacgt caccgtagat ttttaaatcg
    71341 ccataccctg aaagcaggag attaggcggg gtggcgtttt tcttctctga ctccgtcgtt
    71401 gattgaccgt ttaccggcgt gttgccctgt gcggttttaa tttcgcggat ctcggcggct
    71461 tgttgtgtct gaagctgttt aacctgcccc tctgcttttg ctgcccgcat ttctgccgct
    71521 tccaggcgtt cttccagcaa tgccatgcgt tgttccagcg ttaaatgctg tgtttttgcc
    71581 ataccggtac tacatgccaa taataaaccg atggctaaag ataatgtcga ttttttcatt
    71641 atttacgttc cctgaaataa aggagaccct acgctttcca aaaggaatac gtatatagag
    71701 aaatataagg tgtgcacttt cgaattttta atccaacttc agcaggccga tggatagtat
    71761 cccgccatat aatatgagta aatccgctga tttcctggaa cgtgaggaat ataa
//
LOCUS       DAJOTB010000007        61140 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJOTB010000007 DAJOTB010000000
VERSION     DAJOTB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.007"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..234
                     /gene="ghoS"
                     /locus_tag="OHN28_000478"
     CDS             <1..234
                     /gene="ghoS"
                     /locus_tag="OHN28_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2295924.1"
                     /translation="LTEINELNNYLTRDGFLLTMTDDDGNIHELGTNTFGLISTQSEE
                     EIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            340..1200
                     /locus_tag="OHN28_000479"
     CDS             340..1200
                     /locus_tag="OHN28_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCQ2295925.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(1241..1777)
                     /locus_tag="OHN28_000480"
     CDS             complement(1241..1777)
                     /locus_tag="OHN28_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCQ2295926.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            1924..2592
                     /gene="hxpB"
                     /locus_tag="OHN28_000481"
     CDS             1924..2592
                     /gene="hxpB"
                     /locus_tag="OHN28_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCQ2295927.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            2756..3346
                     /locus_tag="OHN28_000482"
     CDS             2756..3346
                     /locus_tag="OHN28_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2295928.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            3479..4870
                     /gene="tcyP"
                     /locus_tag="OHN28_000483"
     CDS             3479..4870
                     /gene="tcyP"
                     /locus_tag="OHN28_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCQ2295929.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(4917..5180)
                     /gene="cedA"
                     /locus_tag="OHN28_000484"
     CDS             complement(4917..5180)
                     /gene="cedA"
                     /locus_tag="OHN28_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCQ2295930.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            5363..7624
                     /gene="katE"
                     /locus_tag="OHN28_000485"
     CDS             5363..7624
                     /gene="katE"
                     /locus_tag="OHN28_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCQ2295931.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(7671..8429)
                     /gene="chbG"
                     /locus_tag="OHN28_000486"
     CDS             complement(7671..8429)
                     /gene="chbG"
                     /locus_tag="OHN28_000486"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCQ2295932.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(8442..9794)
                     /gene="celF"
                     /locus_tag="OHN28_000487"
                     /gene_synonym="chbF"
     CDS             complement(8442..9794)
                     /gene="celF"
                     /locus_tag="OHN28_000487"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCQ2295933.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYQELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(9897..10739)
                     /gene="chbR"
                     /locus_tag="OHN28_000488"
     CDS             complement(9897..10739)
                     /gene="chbR"
                     /locus_tag="OHN28_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCQ2295934.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(10747..11097)
                     /gene="chbA"
                     /locus_tag="OHN28_000489"
     CDS             complement(10747..11097)
                     /gene="chbA"
                     /locus_tag="OHN28_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCQ2295935.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(11148..12506)
                     /gene="chbC"
                     /locus_tag="OHN28_000490"
     CDS             complement(11148..12506)
                     /gene="chbC"
                     /locus_tag="OHN28_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCQ2295936.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(12591..12911)
                     /gene="chbB"
                     /locus_tag="OHN28_000491"
     CDS             complement(12591..12911)
                     /gene="chbB"
                     /locus_tag="OHN28_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCQ2295937.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(13210..13548)
                     /gene="osmE"
                     /locus_tag="OHN28_000492"
     CDS             complement(13210..13548)
                     /gene="osmE"
                     /locus_tag="OHN28_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCQ2295938.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            13750..14577
                     /gene="nadE"
                     /locus_tag="OHN28_000493"
     CDS             13750..14577
                     /gene="nadE"
                     /locus_tag="OHN28_000493"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCQ2295939.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            14807..15694
                     /gene="cho"
                     /locus_tag="OHN28_000494"
     CDS             14807..15694
                     /gene="cho"
                     /locus_tag="OHN28_000494"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCQ2295940.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYQILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(15654..16229)
                     /gene="ves"
                     /locus_tag="OHN28_000495"
     CDS             complement(15654..16229)
                     /gene="ves"
                     /locus_tag="OHN28_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCQ2295941.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(16432..16917)
                     /gene="spy"
                     /locus_tag="OHN28_000496"
     CDS             complement(16432..16917)
                     /gene="spy"
                     /locus_tag="OHN28_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCQ2295942.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(17247..18215)
                     /gene="astE"
                     /locus_tag="OHN28_000497"
     CDS             complement(17247..18215)
                     /gene="astE"
                     /locus_tag="OHN28_000497"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCQ2295943.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(18208..19551)
                     /gene="astB"
                     /locus_tag="OHN28_000498"
     CDS             complement(18208..19551)
                     /gene="astB"
                     /locus_tag="OHN28_000498"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCQ2295944.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(19548..21026)
                     /gene="astD"
                     /locus_tag="OHN28_000499"
     CDS             complement(19548..21026)
                     /gene="astD"
                     /locus_tag="OHN28_000499"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2295945.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(21023..22057)
                     /gene="astA"
                     /locus_tag="OHN28_000500"
     CDS             complement(21023..22057)
                     /gene="astA"
                     /locus_tag="OHN28_000500"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCQ2295946.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(22054..23274)
                     /gene="astC"
                     /locus_tag="OHN28_000501"
     CDS             complement(22054..23274)
                     /gene="astC"
                     /locus_tag="OHN28_000501"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCQ2295947.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
     gene            23720..24526
                     /gene="xthA"
                     /locus_tag="OHN28_000502"
     CDS             23720..24526
                     /gene="xthA"
                     /locus_tag="OHN28_000502"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCQ2295948.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            24693..25403
                     /locus_tag="OHN28_000503"
     CDS             24693..25403
                     /locus_tag="OHN28_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2295949.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            25408..26085
                     /gene="ydjY"
                     /locus_tag="OHN28_000504"
     CDS             25408..26085
                     /gene="ydjY"
                     /locus_tag="OHN28_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCQ2295950.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            26099..26806
                     /locus_tag="OHN28_000505"
     CDS             26099..26806
                     /locus_tag="OHN28_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2295951.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            26806..27354
                     /locus_tag="OHN28_000506"
     CDS             26806..27354
                     /locus_tag="OHN28_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCQ2295952.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            27364..28530
                     /locus_tag="OHN28_000507"
     CDS             27364..28530
                     /locus_tag="OHN28_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2295953.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            28503..30038
                     /locus_tag="OHN28_000508"
     CDS             28503..30038
                     /locus_tag="OHN28_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2295954.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            30038..30691
                     /locus_tag="OHN28_000509"
     CDS             30038..30691
                     /locus_tag="OHN28_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCQ2295955.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            30758..32065
                     /gene="ynjE"
                     /locus_tag="OHN28_000510"
     CDS             30758..32065
                     /gene="ynjE"
                     /locus_tag="OHN28_000510"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCQ2295956.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(32074..32694)
                     /locus_tag="OHN28_000511"
     CDS             complement(32074..32694)
                     /locus_tag="OHN28_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCQ2295957.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            32781..33188
                     /gene="nudG"
                     /locus_tag="OHN28_000512"
     CDS             32781..33188
                     /gene="nudG"
                     /locus_tag="OHN28_000512"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCQ2295958.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(33154..33426)
                     /locus_tag="OHN28_000513"
     CDS             complement(33154..33426)
                     /locus_tag="OHN28_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCQ2295959.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            33662..35005
                     /gene="gdhA"
                     /locus_tag="OHN28_000514"
     CDS             33662..35005
                     /gene="gdhA"
                     /locus_tag="OHN28_000514"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCQ2295960.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(35082..37037)
                     /gene="topB"
                     /locus_tag="OHN28_000515"
     CDS             complement(35082..37037)
                     /gene="topB"
                     /locus_tag="OHN28_000515"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCQ2295961.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(37042..38085)
                     /gene="selD"
                     /locus_tag="OHN28_000516"
     CDS             complement(37042..38085)
                     /gene="selD"
                     /locus_tag="OHN28_000516"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCQ2295962.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGVSFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(38202..38753)
                     /locus_tag="OHN28_000517"
     CDS             complement(38202..38753)
                     /locus_tag="OHN28_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCQ2295963.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            38914..40770
                     /gene="sppA"
                     /locus_tag="OHN28_000518"
     CDS             38914..40770
                     /gene="sppA"
                     /locus_tag="OHN28_000518"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCQ2295964.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            40886..41596
                     /locus_tag="OHN28_000519"
     CDS             40886..41596
                     /locus_tag="OHN28_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HCQ2295965.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            41728..42744
                     /gene="ansA"
                     /locus_tag="OHN28_000520"
     CDS             41728..42744
                     /gene="ansA"
                     /locus_tag="OHN28_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCQ2295966.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            42755..43396
                     /gene="pncA"
                     /locus_tag="OHN28_000521"
     CDS             42755..43396
                     /gene="pncA"
                     /locus_tag="OHN28_000521"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCQ2295967.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(43489..44847)
                     /locus_tag="OHN28_000522"
     CDS             complement(43489..44847)
                     /locus_tag="OHN28_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2295968.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(44965..45723)
                     /locus_tag="OHN28_000523"
     CDS             complement(44965..45723)
                     /locus_tag="OHN28_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2295969.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(45860..46840)
                     /gene="ydjG"
                     /locus_tag="OHN28_000524"
     CDS             complement(45860..46840)
                     /gene="ydjG"
                     /locus_tag="OHN28_000524"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCQ2295970.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(46850..47797)
                     /locus_tag="OHN28_000525"
     CDS             complement(46850..47797)
                     /locus_tag="OHN28_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCQ2295971.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            complement(47802..48638)
                     /locus_tag="OHN28_000526"
     CDS             complement(47802..48638)
                     /locus_tag="OHN28_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCQ2295972.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            complement(48659..49702)
                     /locus_tag="OHN28_000527"
     CDS             complement(48659..49702)
                     /locus_tag="OHN28_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HCQ2295973.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTKDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(49719..51098)
                     /locus_tag="OHN28_000528"
     CDS             complement(49719..51098)
                     /locus_tag="OHN28_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2295974.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(51125..52201)
                     /locus_tag="OHN28_000529"
     CDS             complement(51125..52201)
                     /locus_tag="OHN28_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2295975.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(52571..52843)
                     /locus_tag="OHN28_000530"
     CDS             complement(52571..52843)
                     /locus_tag="OHN28_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCQ2295976.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(52885..53298)
                     /gene="msrB"
                     /locus_tag="OHN28_000531"
     CDS             complement(52885..53298)
                     /gene="msrB"
                     /locus_tag="OHN28_000531"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCQ2295977.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKELSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            53640..54635
                     /gene="gapA"
                     /locus_tag="OHN28_000532"
     CDS             53640..54635
                     /gene="gapA"
                     /locus_tag="OHN28_000532"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2295978.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            54719..55603
                     /locus_tag="OHN28_000533"
     CDS             54719..55603
                     /locus_tag="OHN28_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCQ2295979.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(55654..56508)
                     /gene="yeaE"
                     /locus_tag="OHN28_000534"
     CDS             complement(55654..56508)
                     /gene="yeaE"
                     /locus_tag="OHN28_000534"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCQ2295980.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(56598..57344)
                     /gene="mipA"
                     /locus_tag="OHN28_000535"
     CDS             complement(56598..57344)
                     /gene="mipA"
                     /locus_tag="OHN28_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCQ2295981.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            57780..59714
                     /gene="yeaG"
                     /locus_tag="OHN28_000536"
     CDS             57780..59714
                     /gene="yeaG"
                     /locus_tag="OHN28_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCQ2295982.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            59827..61110
                     /locus_tag="OHN28_000537"
     CDS             59827..61110
                     /locus_tag="OHN28_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCQ2295983.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        15328 a        15885 c        14762 g        15165 t
ORIGIN      
        1 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
       61 acagatgatg atggcaatat ccatgagctg gggactaaca cttttggact tatcagtacc
      121 caaagtgaag aagaaattag agaactggtt tcggggctta cccacagtgc aaccggcaaa
      181 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
      241 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
      301 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
      361 cttttgagcg agcagttagg tgaaggcgaa atagaactgc gtaatgaact gcctggcgga
      421 gaagtccatg ccgcatggca tttgcgctat gcaggacgtg actttttcgt caaatgtgat
      481 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
      541 agtaaaaccg tcaccgtgcc taaggtttgg gcagttggcg ctgaccgtga ctacagtttt
      601 ctggtgatgg attatctccc acctcgcccg ctggatgcgc atagtgcatt tattcttggt
      661 cagcaaattg cgcgtttaca tcaatggagc gaccaaccac aatttggcct cgatttcgat
      721 aacgcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacttttttt
      781 gctgaacaac ggattggctg gcagctggaa ctggcggcag aaaaagggat cgctttcggc
      841 aatatcgatg ccatcgtcga gcatattcag cagcgtcttg cctcgcatca accgcagcct
      901 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
      961 attttcgacc cggcctgtta ctggggtgac cgagagtgcg acctggcgat gttaccgctg
     1021 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     1081 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaaggtta
     1141 tttggcggtc agcatttggt tattgctcag cagtcactgg atagattatt agcagcatga
     1201 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     1261 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatacagc gagaaaaact
     1321 gcaggaaaat agtatggtgc ggaacaacaa tacgtgactc aatttgttca cgcgttagcc
     1381 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     1441 gcgcctggcg actcatccgg gctccggaat cctgcaacgc cagtccgaca aaaatcagga
     1501 tgaaaatcac ccagaacata acgttcacgc caccattaaa attgggcgtc ggagagttgt
     1561 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagtaa
     1621 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     1681 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     1741 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     1801 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     1861 taggcgctta atcataagca cggcttaata ccttacatat aatgctctaa aggagaggtt
     1921 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     1981 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtggat
     2041 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     2101 ctttggtacg cccggcaacc atggaatggg ccaagccgtc aggaagtagt agaacaagtt
     2161 attgcccgtg ccatttcact ggttgaagag acacgtccat tattgccagg tgtgcgtgaa
     2221 gccgttgcgt tatgcaaaga acaaggttta ttggtggggc tggcctccgc gtcaccgctg
     2281 catatgctgg aaaaagtatt gaccatgttt gatttacgcg acagtttcga tgccctcgcc
     2341 tcggctgaaa aactgcctta cagcaagccg catccgcaag tatatcttga ctgcgcagca
     2401 aaactggggg ttgaccctct gacctgcgtg gcactggaag attcggtaaa tggcatgatc
     2461 gcctctaaag cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     2521 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     2581 cttctcggtt aatgaccagg gggcagtgat cacactgccc ctggttcttt atctgaattt
     2641 gcattcaaat gacggattaa tcgccaattt aagagaaaga gttacaccgt caccacttcc
     2701 gtgcactgta taaaaaccct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     2761 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     2821 gacgcccgtg ctggcacagg gggactggtg gcatattgtc ccttccgcaa tcctgacgtg
     2881 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa agtggatatc
     2941 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagcctgc tggcggtatt
     3001 tgcgctgctg gcaacctttt accttaaggt tcctgaaagc tggttcattc cggctgatgc
     3061 gttgcagggt atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     3121 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     3181 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     3241 gccccattca ttacccgaaa acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     3301 acagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatcg
     3361 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     3421 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     3481 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     3541 aacccgccac aaacagtgga gtctggcgaa aaaagtgctg gtgggtcttg tgatgggtgt
     3601 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     3661 tgtacagtgg tttaacattg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     3721 gccgctagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     3781 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcagcgct
     3841 ggttggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     3901 tgcagaaact gcacgtctga acgccatcga aagtaactat gttggtaaag tctctgacct
     3961 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atctgaccgg
     4021 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     4081 gctgaaactg ctgaaggatg atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac
     4141 cctgcaaagc tgggtgatga aactggttcg cctggtcatg cagttgaccc cttacggtgt
     4201 tctggcgctg atgaccaaag tggttgcagg ttctaacctg caagacatca tcaaactggg
     4261 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgcagtgc atggccttct
     4321 gctgggcatt aatggcgtga gcccgctgaa gtacttccgt aaggtatggc cagtactgac
     4381 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     4441 gcgtcgtctg ggtgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcgaccat
     4501 tggtcagaac ggttgtgccg gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac
     4561 ggtgggcatt aacccgctgg acccgatgtg gattgcgacg ctggtcggta ttgtcaccgt
     4621 tagttccgca ggcgttgccg gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct
     4681 gcctgcgatg ggcctgccgg taacactggt ggcattgtta atctccgttg aaccgcttat
     4741 cgacatgggc cgtacggcgc taaacgttag tggctcgatg accgctggca cgctgaccag
     4801 ccagtggctg aagcaaaccg ataaagccat tctggatagc gaagacgacg ccgaactggc
     4861 acaccgttaa tcctgatttg ttacgttaaa cgcctgtcat cccgacaggt gtttttttat
     4921 tcttcgacct cattttcctg gcggatctga tttgcccaac gctgggcgga ttcaggcaca
     4981 gtaaacgccg gtgagcgcag aaatgactcc cccatcagta caaacgcaac atatttgcca
     5041 cgcagcatcc agacatctcg aaacgaatcc atctttatcg catgttctgg cggcgcgggt
     5101 tccgtgcgtg ggacatagct aataatctgg cggttttgct gacggagcgg tttcttcatt
     5161 actggcttca ctaaacgcat attaaaaatc agaaaaactg tagtttagcc gatttagccc
     5221 ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtt tccgaagcgg
     5281 gatctggctg gtggtctata gttagagggt tttttgacca aaacagcggc cctttcagta
     5341 ataatttaag gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca
     5401 gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgaaga
     5461 cggatctcat cgtccagcgg ctgaaccaac accgcctggt gcacaaccta ccgccccagg
     5521 gagcctgaaa gcccctgata cgcgtaacga aaaacttaat tctctggaag acgtacgcaa
     5581 aggcagtgaa aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg acgatcaaaa
     5641 ctcactgcgt gccggtagcc gtggtccaac tctgctggaa gattttattc tgcgcgagaa
     5701 aatcactcac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc
     5761 agctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct
     5821 ctcagatacg aacaaaatca ccccagtatt tgttaggttc tctaccgttc agggcggcgc
     5881 tggctctgcc gataccgtgc gtgatatccg tggctttgcc accaagttct atactgaaga
     5941 gggtattttt gacctcgttg gcaataacac gccaatcttc tttatccagg atgcgcataa
     6001 attccccgat tttgttcatg cggtaaaacc agaaccgcac tgggcagttc cacaagggca
     6061 aagtgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt
     6121 gatgtgggca atgtcggatc gcggtatccc gcgcagttac cgcactatgg aaggcttcgg
     6181 tattcatacc ttccgcctga ttaatgccga agggaaggca acgttcgtac gtttccactg
     6241 gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac taaccggacg
     6301 tgacccggac ttccaccgcc gcgagttgtg ggaagcgatt gaagcaggcg attttcctga
     6361 atacgaactg ggcttccagt tgattcctga agaagacgaa ttcaagttcg acttcgatct
     6421 tctcgatcca accaaactta tcccggaaga attggtgccc gttcagcgtg tcggcaaaat
     6481 ggtgctcaat cgtaatccgg ataacttctt tgctgaaaac gaacaggtgg ctttccatcc
     6541 agggcatatt gtccccggtc tggatttcac caacgacccg ttattgcagg ggcgtttgtt
     6601 ctcttatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg aaattccgat
     6661 taaccgcccg acctgccctt accataattt ccagcgtgac ggcatgcatc gtatggggat
     6721 cgacactaac ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac
     6781 accgccgggg ccaaaacgtg gcggttttga atcataccag gaacgcgtgg aaggcaataa
     6841 agttcgcgag cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct
     6901 aagtcagacg ccattcgagc atcgccatat tgtcgatggt ttcagttttg agttaagcaa
     6961 agtagttcgg ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac
     7021 tctggcccag gcggtggcga aaaatctcgg tatcgaactg actgacgacc agttgaatat
     7081 caccccgcct ccgaacgtca acggtctgaa aaaggatcca tccttaagtc tgtacgccat
     7141 tcctgacggt gatgtgaaag gtcgcgtggt agcaattttg cttaatgatg aagtgagatc
     7201 ggcagacctt ctggccattc tcaaggcgct gaaggccaaa ggcgttcatg ccaaactgct
     7261 ctactcgcga atgggggaag tgactgcgga tgacggtacg gtgttgccta tagctgctac
     7321 ctttgccggt gcgccttcgc tgacggtcga tgcagtcatt gtcccttgcg gcaatattgc
     7381 ggatatcgct aacaacggcg atgccaacta ctatctgatg gaagcctaca aacaccttaa
     7441 accgattgcg ctggcaggag acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca
     7501 gggtgaagaa gggattgcgg aagctgaccg cgccgatggt agttttatgg atgaactgtt
     7561 aacgctgatg acagcacacc gcgtgtggtc acgcattcct aagattgaca aaatcccggc
     7621 gtaaatatgg ataaggtcac gccccctggg gagtgacctt accaccttac ttacacatca
     7681 tgataactac ccaaccgata accacgctca gcaatcgcat atttcaacga cgctgaagtc
     7741 agcacatcca gttccgttaa ccgagggaag caataagcac tctggcgaat cgtattatcg
     7801 ataaacgccg ggtggcacat cacttccagc gagcgctcgc cgcgatgact ggagtcatca
     7861 agcacttgca ggaacagtgt ctcgctaatc tcttcaccat aaaacgcgct actgaatccc
     7921 tgcgaactgc gcagattagc cggtaaatca ccatccttag aaagcgactg acggtcgatg
     7981 cgcaacgcaa tcccctcttc agccgcaaac tttgccacaa tcgggaaaat ctgcgggaac
     8041 atatgcacat gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa
     8101 cgcaaatact gactggcgag ctcctgagta atttcttcca gcggtaaagc atcttcttct
     8161 gccaactgcc agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc
     8221 agaggcttgc ccatagtaag gacaaagtgc atccctatgg ccagactcgg ttcatcacgg
     8281 ctcaactgca ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgacgtgaca
     8341 atcccattgc gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca
     8401 tcagcattaa caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc
     8461 tctgcgatgc agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt
     8521 gccagcagct cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaag
     8581 acatcgttaa attctccact aagtgcggcg ttgctggcag caatctcgaa gcctttgatg
     8641 gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg
     8701 ccatcgcgcc ccagcgtaca ggtcatttct accgcccagt ctgccggaat attatcaata
     8761 tgcccatgat gcgggatatt aacgtaatgt tctgcttgct tgtcgttata gatagcgttg
     8821 atcacttcgc acgcagcatc agaataataa gccccaccgc gctgttccag ttctttcggc
     8881 ttaactttca actccgggtt tttatatagc tcaaaaagtt gtttctcgac tttctgtacg
     8941 acctgcgctc gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttt
     9001 tgcttgaagt aataaagcaa ataagaacac ggcaacagat tcagagaacg aattaagcct
     9061 tcgctaaatg gcagatcgaa aatattttta acgccagatg cttttaactg cccggatgcc
     9121 acaccatcaa gcaattcggc aaagcgtgat ttgccattta ccagcacatc cttaatgaac
     9181 accatatggt tgaggccgaa cagatcgata gataaatcat cgctgtcttt cagcatcaga
     9241 acatcgcgaa taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta
     9301 aatccggtat gacgataaac ggcttcagtg accattcccg ccgggttagt gaagttaatc
     9361 acccatgcat tcggacatag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg
     9421 gtacgcagac ctttaaacag accgcccgcg ccgttagttt cctgaccaag ataaccatga
     9481 cttaatggaa tacgttcatc cagttcccgc gccggtaatt ggccaacgcg cagttgggta
     9541 gtaacgaaat cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc
     9601 gggacgccag cgttatcaat catccgttgg cagagatcaa aaataatatc cagtttcgct
     9661 ttaccacctt cgacatccac cagccataat tcgctgaccg gcaattcctg ataacgctta
     9721 ataaatcctt ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt
     9781 aatttctggc tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc
     9841 ccggacataa ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac
     9901 tgattaaatt cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta
     9961 aacgttttaa taaacaagct cgggctacta taaccggcct caaacgcaat atccgtcact
    10021 gaatagttgg tcatttccag ttgttttttg gcaaaattaa tacggatttc attaataatc
    10081 tgcattggcg ttttaccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg
    10141 gctgacaacg tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatt
    10201 ttttctaccg tacttttcag ccactgcggt atatcatcaa tcacctgttc ttcgcggtaa
    10261 tgacgtaaac ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt
    10321 tcgcggaaat tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc
    10381 cggtatacct gcgaagctac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa
    10441 aagcgtttac tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga
    10501 tggtgcgaac ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg
    10561 ttaatctcct ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga
    10621 tgcagtccgc tgatactctc agttttatta tagataaaca catggaagtt tttgccatta
    10681 aacaactgct gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc
    10741 gactccttat gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc
    10801 aagcatggac gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt
    10861 accttcgcca gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc
    10921 catccgtgac tgatccatca tcgcttttgc tgcggcaaaa tcgccctgct tcgcctgttt
    10981 cagtgccgca tacgccaggc tacgcgcttg tccggagttg atgataagcc ccatcaccac
    11041 ttcttccagc tcttcagctt ccgtttgcgt atcgggaata ttatcgagat ccatcataca
    11101 tcgttcctct ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg
    11161 gcgttagcga tatcttcttc gctctcttct ttatcaatcg cgttttgtgc tttgttagcg
    11221 accacaacaa agggcagata aattaacgtt gcgataccaa ggttgaagag tgcgaccagc
    11281 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
    11341 ggtgcaatat tggtcaccgg tggaataatg cccatgtagt acgctgccag ggtgattgcc
    11401 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
    11461 agaccaaaca gaatcggttc gttaatctgg aagatgccag acggcagcgc cagttttgcc
    11521 acctgacgat aatcagcacg tcgagaggcg ataaagatag ccaggatcag gcctaaagtc
    11581 gcaccactgc caccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
    11641 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
    11701 tccagcgccc aaggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
    11761 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcctac cacgctaccc
    11821 aacgatgcca gtggggttga gatcgtatcc ataatgatct gatggaagtt ggtgccccag
    11881 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaaacc aggaattaat
    11941 gctgagaaag agcgcgaaac cgatgtcgga acgctatcag gtaacttaat gacccagtta
    12001 cgatgaacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
    12061 atattcgccc cacctaacca gtttgcgcca accgcatagg cctcaccgac actatatggg
    12121 gtgacggtca taaatgctgc aacggataac aacccggccg ccagcgcatc gactttacgc
    12181 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt
    12241 ccgttatata cgttgccgcc aatacctttc agaccattaa gtgtttcaat ggttgaggca
    12301 tccaggcgaa tacctaagga ataaaaaaac gacccctccc caaagcttag aaaaacgttg
    12361 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
    12421 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
    12481 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
    12541 tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
    12601 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
    12661 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
    12721 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
    12781 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
    12841 cgcatttttg ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt
    12901 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
    12961 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
    13021 aaggagtata cggtataggc ctgagcaata gtatttcttt gacctgataa attgtctctc
    13081 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
    13141 gaacacaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
    13201 gcgaagtgat tacttcgtag cctgcggatc agtgtcgtat tcagcacagg tctgataacc
    13261 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
    13321 acgttgaccc aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga
    13381 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
    13441 caccacaggc tgtacaaact ggtctttggt acgatcataa gccgtacaac ccgccagcat
    13501 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
    13561 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac
    13621 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
    13681 tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
    13741 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
    13801 aatgctgaag aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat
    13861 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccggt
    13921 aagctgtgcc agatggcgat taatgagctg cgccaggaaa ccggcaacga atcactgcaa
    13981 tttattgcca tacgcctgcc ctatggtgtc caggctgacg aacaagattg ccaggatgcc
    14041 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtactcgcc
    14101 agcgaacagg cgctgcgcga agcgggcatt gaactgagcg attttgtccg tggcaatgaa
    14161 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag tggtgtcgtg
    14221 gtgggcaccg atcatgcagc agaagctatt accggattct tcactaaata tggtgacggc
    14281 ggtacggaca ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg
    14341 gcattaggtt gcccggaaca cctttataag aaagcgccaa cggccgatct tgaagatgat
    14401 cgcccttctc taccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
    14461 ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
    14521 accgaacata aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa
    14581 tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
    14641 gccttactcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg aataggcctg
    14701 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc
    14761 tccccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
    14821 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg
    14881 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
    14941 caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt
    15001 acgcaccccg gatgaagccg ccatgctgcg acaatcccga cgcatcagct ggatatgtac
    15061 cgccggtgaa atcggcgctc tactccttga agcgcgatta atcaaagagc agcagccgct
    15121 gtttaataaa cgcttgcgcc gcaatcgcca gctctgcgcc ctgcaattaa atgaaaagcg
    15181 tgttgatgtg gtgtatgcca aagaggtgga tttttcacga gcctccaacc tgtttggcct
    15241 gtttgccaat aggcgcgcag ctttgcaagc attgcagacc atcgccgatg aacaaaaact
    15301 ttgttatggc ctgttgggac tggaaccgtt aagccgcggt cgtgcatgtt ttcgttctgc
    15361 attaaaacgt tgcgccgggg cgtgttgcgg taaagagagc catgaggaac atgcgctacg
    15421 cttgcgccag gctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gcgcggtggc
    15481 attgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
    15541 gggggcggtt aattcgctga aagaagcgac aacgttaatt cgggcacccg ccgggtttga
    15601 tcacgacggt tatcaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
    15661 acttgatccg gtgaatgacc agcaagccag ttgatttcac tgaacaacag tttaccttgc
    15721 ggttgcaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
    15781 agcagtttgt cgcccaattg ccacgccccg tttattacaa acaccacccc gccgcgtgaa
    15841 ccaaaggtgg taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt
    15901 gtcatgatgt tgaaatccat cgacatctgc cctgccgtca gtttcgcttt taccaccaga
    15961 tccgctgcaa aggaaaaagg ttgtaacggt tttaaggtat ggttaaagcg gtctgcgctt
    16021 tcaaggaaca tctcgccgcc ttccagcaac gtcactatcc tttccatacc gggaaacaaa
    16081 gaaaactccc cattcgccgc gatggatgta atgctggcac gccagtaaaa atcacgtttt
    16141 gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc
    16201 gacattttac gcatatcaaa gtattccata cccgctcccg gcccgttctt ttcattgtta
    16261 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggcgatca
    16321 ggcttttttt gcatctgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
    16381 actgcaaggt agtggacaag accggcggtc ttacgttttt tggctgaaag attattcagc
    16441 agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc
    16501 attaaattgc tttttctgtt ccggcgtcag gatgttgtaa atcttgttct gggtttccat
    16561 gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt
    16621 tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt cttccagtgg
    16681 cggacgtttc atctggtcac gctggccttt catgatttcg cggatctgct gtttctgcgc
    16741 gtcggtcagg ttcaggtctt tgaacatcat gtcctcatgc ggaccgaact tgcctttatg
    16801 gtgcatcatc ggcttcgcgt cagccggtgc tgcagtagtg gtgtctgcgg catgggccag
    16861 gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt
    16921 ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc gtgttgacga
    16981 gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt
    17041 aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag agtgatgaga
    17101 gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga tgactggtga
    17161 taaataaagc aaataaccag gattaatctg tattaattta taagaaagca acttaatacc
    17221 cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa tcccgcacgt
    17281 aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg ggttacggta
    17341 aaacgttcct ccccgtcctg cgccagcaat gttcctttct caaacggcat aaaattcagc
    17401 gtgtcatttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg cgaaaccacc
    17461 cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag cagcgcagca
    17521 attgcgctgg cagttactgc aaactggcga agatcgtttt gtccaaaggg caacgctttg
    17581 ccgagttcca gcgtacaggc cagcgcgcca aaatgtctgg cgctgaaatg ggtaaacgta
    17641 ccaccaggct cctgatggaa caccagtgcc tccagacccg ccgcacccag ccacgtcaga
    17701 aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaaccgcgg atgcaaggag
    17761 ccacgaattg cggtatggag atcaaggtgc cagcgcacag attctttgcc ctggtcataa
    17821 aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttccccgct ttcagcaaat
    17881 agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg tttcccttgc
    17941 ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg gatctcgccg
    18001 tgagatatcg cgccaagcaa cgcatccagc atttccacag gtgccgtctc attaccgtgt
    18061 attcccgctg aaatcaccag tgcgccttgc ggtggcgtta atggcgtcag ttccagcacg
    18121 ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat aaccggtttt
    18181 ttacccgtta aggtcagagc aagaaaatta tccatttccc cctccctcgc gctggaatgg
    18241 ataaaccgaa ccaagatcca ataattgagt cagtgtatcc agcgcttccc gtccttcgcg
    18301 cagcaattgt ggatcggcaa gatccgccgc agtaagacga tcgcggtagt aacgatccac
    18361 ccagtcattg agcgcattaa acagcgtatc gttcatcatc accgccggat tcaccgcccg
    18421 gcgttcttct tctgtcaaca ccacgcgcag ccgcaggcac gccgggccac cgccattcgc
    18481 catgctttca cgtaaatcaa aaacttttag tgagcttatt gggttatcgg cagcaagcaa
    18541 ttcattgaga taacaccata ctccagcatg ttcccgacat tcctgaggca gcaccaacat
    18601 catggaacca tcatcgcggc tcagcagttg gctgttaaac agataagtag acaccgcatc
    18661 tgacacggaa acctgcgccg tgggcacctc tattgccata aaaccactga cccgtgcacg
    18721 cagatttgcc agtaactgtg actggcgagc gaacgcctgt tgatggcaaa acagcacctg
    18781 gcggttactc acggcaatca cgtcattatg aaaaacgccc tggtcgataa cgtccgggtt
    18841 ttgctgggcg aaaatcacct gttggggatt cacctgattc aaccttgcca ccgcctcgct
    18901 ggcttcacgg gtctgccgcg ccggataacg ggaaggccgg gtatcattgc cctcttctcg
    18961 cccgtagaca aaaagttgca tacccggttc accgtaatga ccgccgagac gattgtggtt
    19021 tgccgccccc tcatcaccga gcaaggctac ctgcggcaac gccgaatgga cgctaaattt
    19081 cttttcgtcg ttaaaaatcg cttttaacag cgattcggtg acgggggctt ccagcgaacg
    19141 gtgaaattta ttgttcaggt tagcaacagt gagatgcact ttgccatcca gcgtatcggc
    19201 ggatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
    19261 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
    19321 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcgggatca cggcctgggg
    19381 gaatcccgca tcggcaagtt ttttcatttt cagtaagccc tgttttgccg ccagtcgcgg
    19441 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
    19501 atggtgcgtc agccctacca gcccgtcgaa attgacttcc caggcgttca tcgcaccacc
    19561 tcatcggaaa aatccagccc ggggttaagc gttgcgggca acgttaacga gtccgactcc
    19621 agacttgcca tcggccaggc acaataatct gcggcatacc aggcgctggg gcgatgattg
    19681 ccggaagcgc caatgccgcc gaatggcgcg gtactggcag caccggtaag cggtttgttc
    19741 cagttaacaa tccccgcccg tgcctccagc aacagttgat cgaatttttc ccgctcgggg
    19801 gaaaccagac cgcaagagag gccgaagcga gtgttattcg ccattcgaat cgcttcatcg
    19861 aaattgtcat aacgccagac gcgcaataac ggcccaaaca cttcttcatc cggcacgcca
    19921 gtaacgcctg tcatttcaat gatccccggc gtcagcaacg atgtccctgc ttgtaataag
    19981 cgcggcgcaa gcaggggccg tccgcccatc gcctccagtt cctgccaggc agtaaccacc
    20041 tgctgtgcgg cctgctcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccaa
    20101 gtgcccggcg ttaaccgctg gctgacggca accaggcgag caagaaatgc atcgccctgc
    20161 gttccacttt tcagcaataa acggcgggcg caggtgcagc gttgcccggc agtgacaaac
    20221 gccgactgaa tggtcagatg gacagcggca tcgatatccg ccacctcatc gataattagc
    20281 gggttattac cgcccatctc aagggcgaga attttctccg gctgaccgga aagctggcga
    20341 tgcagctggt agccggtatt ggcgctgccg gtaaacagca aaccgtcgag atcctccagc
    20401 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcagc
    20461 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    20521 ggtttaaaaa taatggtgtt acctgccagc aatgccggaa caatatgtcc gttcggcaga
    20581 tgaccaggga aattatacgg tccaaacact gccagtacgc cgtgcggacg atggcgcaag
    20641 ctcgccgcgc cgtccggcat ttcgctacgc tgctcaccgg tacgaacgtg atacgcctta
    20701 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    20761 ttgccggttt ctctggcaat aatcgcggtt aattcagctt tattgctttc cagcaatccg
    20821 gcaaagcgtt caactcttac ctgacgatcg ctaaatgaga gcctcgccca gcgcggaaac
    20881 gccgctcggg cagcccgaca agcctgctcg acctgagcag catcggcatc attgccctgc
    20941 cataacacct cgcccgatac cggattacgc ttcacacgca atgcgccctg gcccgttacc
    21001 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    21061 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    21121 gctcggtcgc tggatcggta cgcaccagca ccacgcggaa atggtgataa ttttcattgg
    21181 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttcggccact tccaccagcc
    21241 gacttttacg gatggcgcgc acgcggtcga tatcacactc aagtgtcggc ccgccgtcaa
    21301 agatgtcgat atagttacgg tagcgaaaac cttctttctc cagcaccgca cgggcaggtg
    21361 cggtttgcgg atgtacctga ccgatgacgt cctgtgcttc ctgggataaa aagtgcgtat
    21421 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    21481 aatcagcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    21541 aatagccgtg ttcgtcaatc accccgcgca tttcggcgac cactttgtca ttaaacttgt
    21601 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    21661 tgcgccactc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    21721 tgagaaacag cgtcggcaat gcgttataga cattcagctc tttcgaagcg tgaaccagcg
    21781 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atggcgcaaa
    21841 tccccgccac agtgcctgac tcgctatctt ccagtacaaa cacataaccc tgctcacttt
    21901 tgggcagttc gccttgccag gttttgattg ccctttcaat acgcaccgaa agcgtggctt
    21961 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    22021 agacatctga gcgctcaacg ggacgaatga ccatcatgat gaacctcggc taacaaagtg
    22081 ttcgcaagca gctgcaaagc gctccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    22141 cgctggcgca aaacgcacca cgttgccacc cgcaatcagc accatcacgc ctgctttcgc
    22201 cgcttcctga gagagctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    22261 caaaagacct aagccgcgaa cttcactgaa caaactgtag tggtgattaa tggaatttag
    22321 acgctcaaca aaccagtcgt gacgctgttt aaccccgtta agcatctctg gcgtgttgat
    22381 gagatccagc actttgcctg ccaccgccga ggccagcgga ttgccgccat aggtggtgcc
    22441 gtgagtgcca acggtcatca cgctggcgca ctcttcggtt gccaacaacg caccaaccgg
    22501 gaaaccgccg cccagcgctt tggcggtagt taacagatca ggcgtcacgc cgtagtgcat
    22561 ataggcatac agttccccgg tgcgcccgac accggtttgt acttcatcaa agataagcaa
    22621 cgcattgtgg cggtcacaca attcacgcag accttgtaaa aatgcgttgc tggctggcac
    22681 cacaccgcct tccccctgga tgggttcgac aatcaccgca caggtggcgt cgtcaatcag
    22741 cgcactggca gagttaatat cgttatatgc agcatgacga atatccggcg gcagtggcgc
    22801 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    22861 aaacgcattt ttgaacgcca cgatgccgct cttatggctg ccgtagcggt cgtgagcgaa
    22921 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    22981 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    23041 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgcct cacgcagttc
    23101 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    23161 cccctgctgc tcccacaagc gcgaaccttc gccacgtacc ggtataaacg gtgccggagc
    23221 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    23281 ctctacagta aataattagt tatttatatg ttaataataa gtaatgtttg cgctgtaaat
    23341 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    23401 gaaatcaagc agttataacc acatcatatg catttaaagt gcatataaag tgaatacgtt
    23461 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    23521 tctggaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcaacg
    23581 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    23641 tcacttaaca acaggcggta agcaaagcga aattctgcta ccatccacgc actctttatc
    23701 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    23761 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    23821 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    23881 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    23941 ttccgtgcgt cgcggctttc ccggtgacga cgaagaggcg caacggcgga ttattatggc
    24001 ggaaatcccc tcgccgctgg gcaatatcac cgtgatcaac ggttacttcc cgcagggtga
    24061 aagccgcgac catccgataa aattcccggc aaaggcgcag ttttatcaga atctgcaaaa
    24121 ctacctggaa accgaactca agcgtgaaaa tccggtgctg attatgggcg atatgaatat
    24181 cagtcctggc gacctggata ttggcattgg cgaagagaac cgtaagcgct ggctgcgtac
    24241 cggaaaatgt tctttccttc cggaggaacg cgaatggatg gagaggctga tgagctgggg
    24301 gttggtcgat accttccgcc atgcgaatcc gcaaacagca gatcgtttct catggtttga
    24361 ttaccgctca aaaggttttg acgataatcg cggtctgcgc atcgacctgc tgctcgccag
    24421 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    24481 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gctgatctcc
    24541 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    24601 tttgttgccc gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    24661 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaaa tttctttttg
    24721 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    24781 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggctatagcc
    24841 tctatattct gttattcatc attgccaccc tctttctgtt accaggaagc atactggtga
    24901 tcgcgggagg aatagttttt ggcccgctct tagggacact actatcatta attgccgcca
    24961 cgctggcctc ctcgtgctca ttccttctgg cgcgctggct gggacgtgat ttactgctga
    25021 aatacgttgg ccatagccat acctttcagg ccattgaaaa aggcattgcg cgtaacggta
    25081 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    25141 cttacggatt aaccacaatc gccttctggc cttataccct tatttcggca ctcacgaccc
    25201 tacccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    25261 tgcgctttat tttacaactc tgtctggcgg gcctggcact gtttattctc gtccagctcg
    25321 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttctcgccgc agcccacttt
    25381 ctcacccaaa aaatgaagga tagaacgatg ttgcaacatt attcagtgtc atggaaaaaa
    25441 ggactggctg cactctgctt actggctgtt gctgggctta gcggctgcga tcaaaaagag
    25501 aatgcggctg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgtgtcgat
    25561 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    25621 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatgggt
    25681 tatgcgaccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    25741 aacatgacaa tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    25801 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    25861 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    25921 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    25981 tatggtgcgg tggaaaaacg cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    26041 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcag gataaaggat
    26101 gatgaagatg caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    26161 cgctgtgctg ctggcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    26221 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    26281 actagcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    26341 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttact
    26401 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    26461 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtactt gacagtatgg acggcttttt
    26521 cactcgctac ggcaaacaca ccatcctggt atgtcggtta ttgccttttg tccctttcga
    26581 tccaatcagc tatgctgccg gtttgacttc aatacgtttt cgctcgtttt ttatcgccac
    26641 cgggcttggt caattaccgg cgactattgt ttattcctgg gcgggcagca tgttaacagg
    26701 cggtactttc tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt
    26761 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gcctgatggg tttaccgccg
    26821 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    26881 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tctggtgtcg
    26941 atgtttgtcg gctggctgga gcgcaaacgc tcaccgctcg atccggtagt acgatcgctt
    27001 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg
    27061 aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    27121 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgta
    27181 acgccgccaa cggtcgatga tgctctgcgc gcccgactgg ctacgcattt tgacgctcag
    27241 gcgctcaccg aactgacggc attgataggc ctgcaaaatc tgtcagcccg ttttaattct
    27301 gccatggaca ttcccgctca ggggctgtgc cgtattcctg aaaaacgttc ttaaggagag
    27361 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    27421 gccagtgact ggcaggaaat aaaaaatgag gccaaaggac aaaccgtctg gtttaacgcc
    27481 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    27541 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaaacgc
    27601 attcagaccg aagttgctgc cggacgtaaa acgggcggtt cggtggatct gctctgggtg
    27661 aacggcgaaa acttccgcac cttaaaagag gccaatttat tacaaaccgg ctgggcggag
    27721 actctgccca actggcgcta tgtcgacaca cagcttccgg tgcgggaaga tttttcagtg
    27781 cctacagaag gggctgaatc gccctggggc ggcgcacaac tgacgtttat cgcccgccgc
    27841 gatgttacgc tacagccacc acaaacgccg caagccttac tggagtttgc taaagccaat
    27901 cccggcacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag
    27961 ttgctgatta tgctaacgcc agatcccaca gcattaaaag aagcgccgga cgatgccact
    28021 ttcgcccgcg tcactgcgcc cttgtggcaa tatcttgatg cactgcatcc gtatttgtgg
    28081 cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg atgctctgct gaaagctaac
    28141 acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc
    28201 gatttgcctg caagcagtta cagttttggt tttcgcgagg ggatgattgg caacgtccat
    28261 ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc
    28321 ctgctctcac ccgatgcgca actgcgtaaa gcagatcctg ctgtctgggg cgatccttct
    28381 gttctcgacc cgcaaaaact gcctgacggg cagcgcgaaa cattgcaatc gagaatgccg
    28441 caggatctgc cgccggtact ggctgaaccg cacgcaggtt gggtaaatgc gctggaacaa
    28501 gaatggctac accgttacgg tacgcattaa tctttttgct gtgggcgatg atagcggtga
    28561 tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgctttg tcgttgacgc
    28621 actggcaggc gttatttgcc gatccacagt taccgcaggc attactggcg acgctggtgt
    28681 cgacaaccat tgcggcggtc ggggcgttgt tgattgccct gctggtgatt gtggcgttgt
    28741 ggccagggcc gaaatggcag cgtatgtgcg cccgtctgcc gtggctgctc gccattcccc
    28801 atgtggcttt tgccaccagc gcccttctgc tctttgctga cggagggctg ctttatgact
    28861 atttcccgta tttcactccg ccaatggaca aattgggtat tgggctgggc ctcaccctgg
    28921 cggtgaaaga aagcgcattt ctgctgtgga tcttagcggc agtattgagc gaaaaacggc
    28981 tgttgcagca actcattgtg ctggattcgc tgggctacag ccgctggcaa tgcctgaact
    29041 ggctgctgtt gccctccgtc gcgtctgcgt tagcaatggc gatgctggcg attgtcgcct
    29101 ggtcgctgtc ggtcgtggat gtggcgatta ttcttgggcc aggtaatccc ccgacgctgg
    29161 cggtaattag ctggcagtgg ttaactcagg gcgacgccga tcaacaaacg aaaggcgcac
    29221 tcgccagcct gctgctgatg ctgttactcg ccgcctacgt tttgctgggc tatctgctat
    29281 ggcgtggctg gcggcgtact attccccgcg tagatggcgt tcgtaagcct gccacgcctt
    29341 tattgccaag caatacgctg gcgagttttt tacccttaac cggtgtgctg tgtgtggttc
    29401 tgctggcgat cctcgcggat cagtcgacga tcaatagtca agcgctcatc aacagcctga
    29461 caatggggct ggtggcggca ttcatcgctt tgctcttgtt actgctgtgg ctggaatggg
    29521 ggccacagcg tcgccagttg tggctatggt tgcccatttt attacccgct ctgccgctgg
    29581 tggcgggcca gtacacgctg gcgctatggc tgaacctgga tggaagctgg acggcggtgg
    29641 tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt tatcctgcaa cccgcctggc
    29701 agcgcattga ttcacggtta attttgattg cgcaaacact gggctggtcg cgggccaaaa
    29761 tcttctttta cgtaaaatgc ccactcatgt tgcgccctgc gctgattgcc ttcgcggtgg
    29821 gattttcagt cagtattgcg cagtatatgc cgacgctgtg gctgggcgcg gggcgttttc
    29881 cgacgctcac cactgaagcg gtggcattaa gcagcggcgg cagcaacggt attctcgccg
    29941 cccaggcttt atggcaactg ctgttaccgc ttattatttt tgccctgacc gcgttagtcg
    30001 caaaatgggt aggttatgtc agacaaggac tccgctaatg ctctgcgtga aaaatgtttc
    30061 gctacgttta ccagaaagcc gcttgctgac aaacgttaac tttacagtag ataaaggtga
    30121 cattgtcacg ttaatgggac catccggctg cgggaaatcc actctgtttt cgtggatgat
    30181 tggtgcgctg gccggacagt tttcttgtac aggggagcta tggcttaatg agcaacggat
    30241 tgacatgcta cccaccgcac agcgtcagat tggcattctt ttccaggatg cactgttatt
    30301 tgaccagttc agcgtcgggc aaaatttact gctggcgcta ccggcgacac ttaaggggaa
    30361 tgcccgacgt aatgccgtga atgatgcact tgagcgttca ggccttgacg gagcgttcca
    30421 tcaggatcct gccactttgt ctggcggtca gcgagcgcgc gttgctctgc ttcgcgccct
    30481 tctcgctcag ccaaaagcgt tgctactgga tgagccattc agccgtcttg atgtggccct
    30541 gcgcgataat tttcgccatt gggtgttcag cgaaattcgc gccatggcga tccctgttgt
    30601 gcaggtgacg cacgatcttc aggatgttcc tcctgatagc cccgttctgg atatggcgca
    30661 gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttcccgatg cgtcagttca
    30721 gaatggcgct ctcaaaacta caatgtcggg attttcgatg aaacgtgttt ctcaaatgac
    30781 cgcgctggca atggctttag ggctggcttg cgcttcttcg tgggccgctg aactggcgaa
    30841 gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata ctcgccccag
    30901 cgcgttttat aacggctggc cacaaacctt aaatggccct tccggtcatg aacctgccgc
    30961 cttaaacctc tctgctagct ggctcgacaa aatgagcacc gaacagctca acgagtggat
    31021 caagcaacat aacctgaaag ccgatgcccc ggtggcactg tacggtaatg acaaagatgt
    31081 cgacgccgtc aaaacgcgac tgcaaaaagc aggttttacg catatctcca tcctgagtga
    31141 cgcgctaagc gaaccttccc gtctgcaaaa actgccgcac tttgaacagc tggtatatcc
    31201 gcagtggctg catgacctgc aacaaggtaa agaggttacg gcgaaacctg ccggtgactg
    31261 gaaagtcatt gaagcggcct ggggcgctcc aaaattttac cttatcagcc atattcccgg
    31321 cgctgactac atcgacacca acgaagtgga aagcgaaccg ctgtggaaca aagtttctga
    31381 tgaacaactg aaagcgatgc tggcaaaaca cggcattcgc catgacacca cggtcattct
    31441 gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcaa attatgcttt atgccggcgt
    31501 gaaagatgtg cgcctgctgg acggaggctg gcaaacctgg tccgacgcgg gtctgcccgt
    31561 tgagcgcggt acgccaccga aagtgaaagc ggaaccggat ttcggcgtga agatcccggc
    31621 acaaccgcaa ctgatgcttg atatggaaca agcgcgtgga ctgctgcatc gccaggatgc
    31681 atcgctggtg agcattcgtt cgtggccaga atttatcggt acgaccagcg gttacagcta
    31741 tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgcaggta gcgactcgac
    31801 gcatatggaa gatttccata acccggatgg caccatgcgc agcgccgatg atataaccgc
    31861 tatgtggaaa gcatggaata tcaaaccaga tcagcaagtt tcattctact gcggcactgg
    31921 ctggcgcgcg tcagaaacct ttatgtacgc acgcgcaatg ggctggaata acgtctccgt
    31981 ttacgacggt ggctggtacg aatggagcag cgatccaaaa aatccggtag caaccggtga
    32041 acgcggcccg gacagcagta aataacattg caattactga cgctggagcg acttcagcgt
    32101 cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac caaataccca
    32161 cgcaaaccac ggaaaacagg ccggaaagag gcaactcaga acaaacagca aaatcgtctc
    32221 gctgccttcg gttaagccgc ccagataata aaacgattta tgcgcataac caggattatc
    32281 aatctgatgt ttcgccgcca gcgcagcaaa agcgagaaaa ctgctgcccg tgccgataaa
    32341 cgcaaacagc agccagccgc ctgccagggc gttttgctcc ggtgcggcga ggataaaccc
    32401 aaaaggcacc agcgcgtaaa aaagaaaatc gagagaaata tcgagaaagc cgcccgcgtc
    32461 ggtaagccct ctgcgccgcg ccagcgcacc gtccagacca tcaagcagcc tgttcaacaa
    32521 aatggcgact agtgccgcca gataccagcc cagcgccaga aaaggcagtg ccagcacgcc
    32581 gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat caagaacccg
    32641 cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta gcacaggcac
    32701 tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct
    32761 tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc atcgaacgtg
    32821 atggcaaaat tttactcgcg caacgccctg cccagagcga tcaggcggga ttatgggagt
    32881 ttgccggtgg taaaatcgag ccggatgaaa gccagcggca ggcgctggtg cgtgagttaa
    32941 acgaagaact gggcatcgaa gcaactgttg gtgactatgt tgccagccat cagcgagaag
    33001 tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac
    33061 aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg cgatatccgc
    33121 tggcccctgc tgacattcca ttattagagg cgtttatggc ttcacgcgcc gccagaccag
    33181 cggattagtg cttagggttt tgtcatcccg ttggcattgc agcagtattc cttcggcttt
    33241 aattactgcc ccttcagaat aattttgatc ctgataaacg cagcactgag tacagggctg
    33301 cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca cgtccggatg
    33361 ataatgcggg ttagccagtg taattaatgg aaatgctaat actacggcga tcaatgctcg
    33421 actcacaggg aactccttaa cgttattgtc tcttctacag ataacggtag ccgggcggca
    33481 aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta ttaatttgct
    33541 ttcctgggtc atttttttct tgcttaccgt cacattcttg atggtatagt cgaaaactgc
    33601 aaaagcacat gacataaaca acataagcat aatcgtatta atatataagg gttttatatc
    33661 tatggatcag acatattctc tggagtcatt cctcaaccat gtccaaaagc gcgacccgaa
    33721 tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc acactctggc cttttcttga
    33781 acaaaatccc aaatatcgtc agatgtcatt actggagcgt ctggttgaac cggagcgcgt
    33841 gatccaattt cgcgtagtat gggttgatga tcgcaaccag gtgcaggtca accgtgcatg
    33901 gcgtgtgcaa ttcagctccg ccatcggtcc gtacaaagga ggtatgcgct tccacccttc
    33961 agtaaacctt tccattctca aattcctcgg cttcgagcaa acctttaaaa atgccctgac
    34021 tactctgccg atgggtggtg gtaaaggcgg cagcgacttt gatccgaaag ggaaaagcga
    34081 aggcgaagtt atgcgtttct gccaggcgct gatgactgaa ctgtatcgcc acctgggcgc
    34141 ggatactgac gttccggcag gtgatatcgg cgttggtggt cgtgaagtcg gctttatggc
    34201 ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc ttcaccggta agggcctttc
    34261 atttggcggc agtcttattc gcccggaagc taccggctac ggtctggttt atttcacaga
    34321 agcaatgcta aaacgccacg gtatgggttt tgaagggatg cgcgtttccg tttctggctc
    34381 cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc gagtgatcac
    34441 tgcgtcagac tccagcggca ctgtagttga tgaaagcgga ttcacgaaag agaaactggc
    34501 gcgccttatc gaaatcaaag ccagccgcga tggtcgagtg gcagattacg ccaaagaatt
    34561 tggtctggtc tatctcgaag gccaacaacc gtggtctgta ccggttgata tcgccctgcc
    34621 ttgcgccacc cagaacgaac tggatgttga cgccgcgcat cagcttatcg ccaatggcgt
    34681 taaagccgtc gccgaagggg caaatatgcc gaccaccatc gaagcgactg aactgttcca
    34741 gcaggcaggc gtactgtttg caccgggtaa agcggctaat gctggcggtg tagcaacatc
    34801 gggcctggaa atggcgcaaa acgctgcgcg cctgggctgg aaagccgaga aagttgacgc
    34861 acgtttgcat cacatcatgc tggatatcca ccatgcctgt gttgagcatg gtggtgaagg
    34921 tgagcaaacc aactacgtgc agggcgcgaa cattgccggt tttgtgaagg ttgcggatgc
    34981 gatgttaagc cagggcgtga tttaagacaa ttgtgccgga tacagcgcaa acgccttatc
    35041 cggccataaa aatcttcagt atcagatggt tgaaagaacc tttatattat cactccattg
    35101 cccgcctctt ctgatggcgg gctttttttc ggcgctctct tgcgtggcga atcctttttc
    35161 ttcccttctc caccgccggg agccattatt ccccgaaact gccttaccga cgtgcgcttt
    35221 gcctgatcaa tcaactggta caatgtcccc accagcggct gcataaagtc ctgatagcga
    35281 cactgctttt cgctgatttg cgtcagcacc gattcccagt gcgcggtcat gtccggtcgc
    35341 gtcgccattt ctggcagcga atggaatagc gcttttccgg cgtcggtgga gtggatatag
    35401 cgcccttttt tggtcaggaa accccgcttg aacaacagtt caataatccc ggcacgcgtt
    35461 gcttctgtcc ccagaccatc ggtcgcacga aggatctttt tcagatcttt gtcctgcaca
    35521 aagcgcgcga tcccggtcat cgccgaaagc agtgttgcat cggtaaaatg gcgcggcggc
    35581 tgggtttgcc gctctaccac ttcgcctttt tcgcacaaca attcatcgcc tttcgccacc
    35641 acaggcagcg gcgtgccgtc gttttcttca tcgcgctctt tgctgcctaa cagcgtgcgc
    35701 cagcctgctt cagcaagaaa acgggcttta gcgacaaatt tgcctttggc aatgtccagt
    35761 tcgataacac acttgcggaa caccgcatcc gggcagaact gcatcaagta ctgacgggca
    35821 atcaggttat agaccttcgc ttcgttctcc gtcaggttga tcgcagaact ccgtgcggtc
    35881 ggaatgatgg cgtggtgcgc atcgaccttt ttgtcatccc aacagcggtt gcgtatatct
    35941 ggatctacca ctggctgcgg caacagatcc ggtgcatgaa cgctgatagc attcatcacc
    36001 gcgtggcgtc cggcaaaatg ttcttctggc aaatagcggc aatcggaacg cggataagtg
    36061 attagcttgt acgtttcgta cagcttctgg cagatatcaa gcacgttctg cgcgctcaga
    36121 ccaaaacgtt tagccgcttc aatctgcaac gccgaaagcg aaaaaggcag cggcgcggat
    36181 tctgattccc gtttatcgtt atagctggtg acaatagctg gttgaccact aatacggtta
    36241 accacatgct ccgccagtgg acgatgtaac aagcgccctt cttcatcctg gtacggttca
    36301 cacgcttcgc tcggttgcca gatagcggta aaccgctcat cggcaggtgt cacgatatgt
    36361 gctttgactt caaagaagtc tttcgccacg aagttttcaa tctcttcgtc gcggcgcacc
    36421 accagtccga gtacgggcgt ctgcacgcgt cccacggaaa gcacaccctg ataaccggca
    36481 ttgcggccga gaatggtata cgcacgggtc atattaatgc cgtacagcca gtcagcccgc
    36541 gcccgcgcca gtgcagaaac gcacagcggt acaaactcgc tattggagcg aagacgatcg
    36601 atcgcccgct caaccgcctg cgggttcaag tcgtttatca aacaacgctg tacctgctgg
    36661 cgcttttccg gtgccagttg caggtagtcc agcacttcat ccaccagcag ttgcccttcg
    36721 cgatccgggt ctccggcgtg aacgatttcg ctggcttcat gcaggaagcg tttgatgaca
    36781 ttaagttgtt tagtcacgga gggtcggggc tgtaattgcc acttttccgg gacaattggc
    36841 aaatccgcaa gattccagcg cgcatagcgg ctgtcgtagg cgtctggctg cgcctgctca
    36901 agcaggtgac cgatacacca ggtcaccacc tgaccattac cgcactcgat aaagccatcg
    36961 cctttccggt gcggtttagg caggacatca gcaatggcgc gcgccagact cggtttttcg
    37021 gcaataaaca accgcattga attaacggat ctcaaccatg gcacgaccgc cgcgcgctgg
    37081 caccagttcg ccaatcgccg tcagttcaat gccaaactcg gcggctgtag ctttgacctc
    37141 attttctgct tccggcatga ccgccagcag caaaccgcca gaagtttgcg gatcgcacag
    37201 cagatcgcgc acttcacgcg gcatttcacc catcagatga ccgtagctgg caaagttacg
    37261 ttcggtgccg ccaggcactg cgcccaactt aatgtactct tcaacccccg gcaatttcgg
    37321 gatcgcgtca tagtcgacgc gcgcctgcac acctgcgccc tgacacattt cgcttaaatg
    37381 ccccagtaaa ccaaaacccg taacgtcggt catcgctttt acgccttcga tgttggcaaa
    37441 ggacacgcct gcgatgttca tccggcacat cacttccgtc gccagtccct gatgttctgg
    37501 tttcagcagt gattttttct cagccgtggt aagaacgcca atccccagcg gtttcgttag
    37561 gaacagtttg catccggctt gtgcggtact gtttttcttc acccgctcgg tcggtacgat
    37621 ccccgttacc gccagaccaa aaatcggctc cggcgcatcg atggagtgac cgccagccag
    37681 cgcaataccc gcctgacggc atgcatagcg tccaccttcg gtcacttcgc gggcaatttc
    37741 cggggaaagt ttgttaatcg gccagccgag gatcgcaatc gccataatcg gtttgccgcc
    37801 catagcaaag atatcgctga tggcgttagt cgccgcaatg cggccaaaat cgaaaggatt
    37861 atcaacgatc ggcataaaga agtcggtggt actgataacg ctggtgccat tgcccagatc
    37921 gtacaccgcc gcatcgtcgc gggtttcatt acccacaagc aaattcggat caacaaactt
    37981 cgcctgctca ctgtgcagga tggtttccaa cacttttggg gaaattttac agccgcaacc
    38041 agcgccgtgg ctgtattggg tcaaacgaat cgagttctcg ctcatggaca tctcctgtca
    38101 atgcaatccg ggtatggtaa ccctcattcc atgaagtgat aagtgagagt gtctgaattc
    38161 ctgcgccttt gctcacaatc cagacagttt cgcgacaatt atcagaaata agtcacaaac
    38221 ggcgtcgggt ccgggacgtt tatcgacgta gatgctttca actgcggcgt accgaggtag
    38281 agaaaaccga caattttatc ctgctcacgg caaccgaatg cttcacgcac taccggactt
    38341 tcagttaatg caccactgcg ccagatgccg ccaaacccct gggcaactgc tgccatttgc
    38401 atcgccatga ccgcgcatcc cgcagacatt tcctgttccc agcgcgggac tttatgattt
    38461 tcttcacatt tcgccaccac cgttatgatg agcggtgcgc ggaacggcgc attacgcgct
    38521 ttgtcgattg ctttgttatc actaccggca gcaatcgccc cctgctccag tacggcgctg
    38581 aaacgctcgc gcccttcccc ttcaatcaca aagaaatgcc acggttgcat ggacttatgg
    38641 tccggcgcac gcatacccgc acgcaggatg ttttgcagtt gttcacccgt tggcgcgggt
    38701 tcagccaggc gggaggcgct acggcgattg atcaatagtt cgagtgcatc catttgatta
    38761 actcctgtca tgatatttat tcacaaaatt aacacgagag tggattttgt tacagcccag
    38821 tccgcaattc ctgctgacaa gtgccggttg ggtcattacg ataaccacat ctattgcgcc
    38881 tgaaacaggt gtgaccttaa gttgggagaa tacatgcgaa ccctttggcg atttattgcc
    38941 ggatttttta aatggacgtg gcgtctgctg aatttcgtcc gtgaaatggt acttaacctg
    39001 ttctttattt ttctcgtgct ggttggtgtg gggatctgga tgcaggtcag tggtggtgat
    39061 tcgaaagaaa cggccagtcg tggcgcactg ctgctggaca tttctggtgt gatcgtcgat
    39121 aaacccgaca gttctcagcg gtttagtaaa ttaagccgcc agctgcttgg tgccagttcc
    39181 gatcgtcttc aggaaaactc actgtttgat atcgtcaaca ccattcgcca ggcgaaggac
    39241 gaccgcaata tcaccggtat tgtgatggat ctgaaaaact tcgcaggcgg cgaccagccg
    39301 tctatgcagt acatcggcaa agctctgaaa gagtttcgtg acagcgggaa accggtttat
    39361 gccgttggcg agaactacag ccaggggcaa tattatctcg ccagtttcgc caataaaatt
    39421 tggctgtctc cgcaaggcgt ggttgatctg cacggctttg ccaccaacgg tctgtactac
    39481 aaatcgttac tggataagct gaaagtttcc acccatgtgt tccgcgtggg tacgtataaa
    39541 tctgccgttg aaccgtttat tcgtgatgat atgtcgccgg cagcccgcga agctgacagc
    39601 cgctggattg gtgagctgtg gcaaaactat ctgaatactg ttgccgctaa ccgccagatc
    39661 cctgctcagc aggtattccc tggcgcgcaa gggttgcttg agggtttaac caaaaccggt
    39721 ggcgataccg cgaaatatgc gctggaaaac aagctggtcg atgcactggc atcgagtgcc
    39781 gaaatcgaaa aaactctgac caaagagttc ggctggagta aaactgataa aaattatcgc
    39841 gccatcagtt attacgatta cgcattgaaa acgccggcag ataccggtga cagtatcggt
    39901 gtcgtcttcg ctaatggcgc aattatggat ggcgaggaaa ctcaggggaa tgttggcggt
    39961 gataccactg cggcacaaat ccgcgacgct cgccttgacc cgaaagtgaa agcgattgtt
    40021 ctgcgtgtta atagtcccgg cggcagcgtt accgcgtctg aagtgattcg cgctgaactg
    40081 gcagcagccc gggcagcggg taagcctgtg gttgtatcga tgggtggcat ggcggcatct
    40141 ggtggttact ggatttccac accagctaat tacattgtgg ctaaccccag caccctgacc
    40201 ggttctatcg gtatcttcgg cgtgatcacc accgtagaaa atagtctgga ttcgattggt
    40261 gttcatactg atggtgtctc aacttcaccg ctggcggatg tttctatcac cagggcactg
    40321 ccgccggaag tgcagcagat gatgcagtta agcattgaga atggctataa acgctttatc
    40381 acgctggttg ctgatgcgcg tcattcaacg ccggagcaga ttgataaaat cgcccagggc
    40441 cacgtctgga ccggtcagga tgcaaaagct aacgggctgg tcgatagtct cggggatttc
    40501 gatgatgcgg tcgccaaagc agcagagctg gcaaaagtga aacagtggca tctggaatac
    40561 tacgttgatg aaccgacctt cttcgacaaa gtgatggaca acatgtctgg ttctgtccgg
    40621 gcaatgttgc cagatgcgtt ccaggccatg ttacctgcac cgctggcctc ggtagcctct
    40681 accgttaaaa gtgaaagcga caagctggcc gcgtttaacg acccacaaaa ccgttatgcg
    40741 ttttgcctga cctgcgccaa cgtgcgttaa gtcctgcaaa aatgtggccg atatgctgtc
    40801 ggccacactc ctgaatcgga ctgtctttca ctccctctct ccactacatt cccttgctca
    40861 tttagcatct gatatggagt catcagtgaa taaggccccc tctttaattg ccgctatcgt
    40921 cctcggactg gggattagcg cctgtggtta ttttgttggc gatggtgtta aacatttaaa
    40981 aaccaataac cgttatgtaa atgtgcgtgg gctttctgaa aaagaagttc gcgcagatac
    41041 ggcagaatta acaattgcga ttaactttaa gggtaatatt cccggcgaac tgttcccgaa
    41101 actggaagac gcgcagaaaa aaattgttgc cgaactcaat gctcagggga ttaacgagaa
    41161 agagataacc ctgggccaat ggacaagcaa gcgaacagat tccttttatc tgaaagatga
    41221 tcccacaatg ccacgctata acgcagacgg gtcagtgacg ataaaaaccc ataatgttgc
    41281 tgctgtcgaa aaagtcgtcg cgaaattaaa cgaactgcaa gtcgctacgg agggcgctat
    41341 cgctgagagc aaagtcgcct atcgctttaa tggtattggc gcactgcgcg ctgagatgat
    41401 tgccgccgcc accaaagatg cacgcaacgc agcattacaa tttgcaacgg actcaggtag
    41461 ccaggtgggg tcaattagcg atgcctcaca aggcgtgttc cagatcttcg ccagtggcag
    41521 tgatgaagat gatcctacgg cgattaataa aacggtacgc gtagtcacca ccgtaaccta
    41581 tgcgttgcag gattaaccac ttttctccgg cgggacacta atccctccgt tctgccggac
    41641 ttttctcata tccacagagt atccaggtgc gctcgctgcc cctcacgtat atacttttgc
    41701 tctttcgata tcattcatat caatatcatg caaaagaaat caatttacgt cgcctacacg
    41761 ggcgggacca tcgggatgca gcgttccgag cagggttata ttccggtgtc aggtcatcta
    41821 cagcgccaac tggcgctgat gccggaattc catcgcccgg agatgccaga tttcaccatt
    41881 catgaatata cgccattgat ggattcttct gatatgacgc cagaagactg gcagcatatt
    41941 gctgaagata ttaaagcgca ctatgacgac tatgatggtt ttgtcattct gcacggcaca
    42001 gacacgatgg cgtataccgc ctctgcactg tcgttcatgc tcgagaatct cagtaaaccg
    42061 gtcattgtga cagggtcaca aatcccgctg gctgagttac gctctgacgg acaaattaat
    42121 ctgctgaatg cgttgtacgt tgcggcgaat tatcctatca acgaagtaac tctctttttt
    42181 aataatcgac tgtatcgtgg caaccgaacc accaaagccc atgccgatgg ttttgatgcg
    42241 tttgcctctc caaaccttcc tccgttactg gaagcaggta tccatattcg tcgtttgaat
    42301 acgccacccg ccccgcacgg cgaaggggaa ttgatcgttc atccaatcac gccgcaacca
    42361 attggcgtag tgacgattta tccggggatt tctgctgacg tcgtgcgcaa ttttctgcgc
    42421 caacctgtga aagcattgat tctgcgctca tatggcgtgg gtaatgcgcc acaaaacaaa
    42481 gccttcctgc aggaattaca agaagccagc gatcgcggta ttgtggtggt caacctgaca
    42541 caatgtatgt ccggtaaagt gaacatgggt ggttatgcca ccggtaacgc cctcgcccat
    42601 gccggcgtta ttggcggtgc agatatgact gtagaagcca cgctaaccaa actgcattac
    42661 ctgcttagcc aggaacttga tactgaaacc attcgcaagg ccatgagcca aaacctgcgt
    42721 ggcgaactga cgccggatga ttaaggagac tgcaatgccc cctcgcgccc tgttactggt
    42781 cgatttacaa aatgatttct gtgctggtgg cgcgctcgcc gtgccggaag gtgacagtac
    42841 ggtggatgtc gctaaccgcc tgattgactg gtgccagtcg cgcggtgaag cagttatcgc
    42901 cagtcaggac tggcatccgg cgaatcacgg cagttttgcc agtcagcacg gtgtagagcc
    42961 ttatacgcca ggccaactcg acggtttgcc acaaaccttc tggcccgatc actgtgtgca
    43021 gaacagtgaa ggcgcacaat tacatccgtt actgaagcaa aaagcgatcg cagcggtgtt
    43081 ccataaaggc gaaaatcctt tagttgacag ttacagtgcc ttttttgata acggtcgtcg
    43141 gcagaaaact gcgctcgatg actggttgcg cgctcatgta atcaatgaat tgatcgttat
    43201 gggtctggct actgactatt gcgttaagtt taccgtgctg gacgctttac agttaggcta
    43261 taaggtgaac gtgattaccg atggttgtcg tggcgtaaat atccagccac aggacagtgc
    43321 gcacgcgttt atggaaatgt cagcagctgg ggcaacgcta tatacgctgg cagactggga
    43381 agaaacgcag gagtaatttt acgctggcct acaattctgt actggcattg taggccagat
    43441 aaaacacatc agtgatacat ctggtaattg acgccatcaa cgaaagattt aattcacctc
    43501 agaaatctct tccagcgaca ccttccgcgt ttcgatgcca aaaatggaga gaaccagcgc
    43561 acagagcact aacatgacac caagcaccat aaacaccgtg atcgacccat aatgtgttaa
    43621 taatgccgca acgccatagg gcgtgaagac tgcgacgatc cgtcctacgg cattaacgaa
    43681 acccgaacca cgcaggcgta aatgcgttgg ccaaagttcc gggatataaa ccgccgacgc
    43741 gaagcaaaca tacatgtata aaaagaagat catcaccagg ccatagatta aaatcgccca
    43801 ctctgttgtc tgaattgaat agatatagcc taacacggca ataataataa gtaaggcgga
    43861 accaaataat cgacgaggga aatgatcaat aattaatgcc gcaataaata tccctaccgg
    43921 agcgccaatc ataataacag cggtcattaa tattgattta tcgacatcaa tgccggagtt
    43981 aacaaatatg gtcggcatcc atacggtgat ggtataaagc gaaatgttca ttgcaattaa
    44041 aacagtaatc gcgactaatg tacgtcgtaa catttcccct ttaaacagga gccagaaggt
    44101 acccttgatt accttaacct tgctgttgct ctggttcaaa gtaagcgggg gtaaacgaat
    44161 actcttctct ctttcaattt gctgctctac ttcacgaagt tggctttctg cacctgcgat
    44221 tcgccctttc cctgccaacc atcgtggcga ctcaataaag tatttacctg agagaaacca
    44281 ggctaacagt atgccaatac cgcccagcag aaacattatc cgccaactaa aaaaagcgat
    44341 aaccaccacg cctatcgccg cagacagcat gggcgaccag tttccaacaa atgagagccg
    44401 cgcggaccat tttccacgca ccgtcgcggg gataaactcc gtaaatgagg catagccaac
    44461 cataatcagc gcccccattc ctgttcccat caggaagcga aagaagatga gccagtacat
    44521 atcagggaca aaagcggccc ctgttgccgc aataccgacg atgagaagat ttatgcgaaa
    44581 cgccctgcgc cgcccaaagt agtcaccaat aaacccaccg gtaagagagc cgatgaaata
    44641 accaaacatt aatgccgagg taaaggcggc attgaggaaa ttatttgacc atccattgct
    44701 taccagcttt gctaagacga cattaccgga gtaactcaaa aaccccgtta ataacagact
    44761 aaagcttatt ataccaaata tacgataatg aaaccgggcc aaaggcaagc ggtccagtct
    44821 tgcgccaatt tgatcatatt gttccattgg gtgcctcgga tttgctaaaa aaggtagcaa
    44881 ttgcggcaaa ttcacttgcc gcatctttat catcatttta tttccaggcg caggaacaaa
    44941 taatattgac aatgtgtgct taaattacca caccagctgg atattattgt ctttgcaaaa
    45001 tgctatccac tctgcacccg gtgatttatc agttattatg taattaatat gactaaagtc
    45061 agctaactgg acaaaagctt tgcgatcaaa cttagagtga tcaaccaata acgcaacttc
    45121 tgtcgcctga cggatcattg tctttttgat ttcagcttcc gcttcattag agtccagcgc
    45181 gccgctgtta atatcaagac ctttacaact cattaccatg atatcgacat ggtagcggct
    45241 gatgatctct ttagtaattc ttccttgcag tgaaagtgta tttttgttta gttccccacc
    45301 cgttgaaacg actttaattt ctgactgagc cagaacatga atcgcttccg ccgagttggt
    45361 tagcaacgtc aggtcactac ggtcctgtaa caatttgagc aattccatta cggtactact
    45421 ggaatcggct gccatggtgg ttttattgtc gacaaagggt agtgccttgc gtgcaataag
    45481 ctgcttctct tcataaaacg atgaagcgcg cttataaaaa tggatattct ccgtcaacat
    45541 cgctgtattt aaaacagcac caccataggt tctggtcaaa aagccttcat cttccaactt
    45601 ctcaagatcg cggcgaatgg tttcttcggt tacctgaaaa atcccactca aatttgagac
    45661 tgtcaccttt ttatcgttgg caaccatttg cttaattgcc tgaatcctgt cttttgccgc
    45721 cacgattaca cccctgtatc tttttacatc acattagcgc aattatcgca taaccgatgt
    45781 ttactttcaa aataacctgt ttgaatcaca gattttcatc acagttttca cagaaacaga
    45841 ggtgaatcgt gttgagtatt taacgctcca gggcctctgc catttccctc atcaatgttg
    45901 cgtctgcatc cgataagttg atattcagtg ccgcgacatt ttcacgtacc tgttccggcg
    45961 cagtagcccc actaagaatg gagattaaat cactctgttt taatatccac gccagtgcca
    46021 gagtgggaat tgtgcactga taacgagcac agagtggctg ccactgttca agcatatcaa
    46081 tcactttcag catgttttca cgctggaacc agactttatt tgcccgagcg ccgcccggaa
    46141 cgtaatcacg cgtgatggtg ccggtcaaca atccctgctc tagcggggaa taaacctgaa
    46201 ccacaatgcc attatcacga catagcggca gcagttcgtt ttccatcgcc cggtcgagaa
    46261 tactgtattt cgcctgaata atatccagtt caccatgttg cagatactcg cggatatggt
    46321 cagcatcgac gttagcagcg cctatagcgc gaattttccc ttcggccttt aacgcattaa
    46381 gcactgcaac agtttcagct atcggcgtaa aaaatggcgg caccgactgc cagtgcgtca
    46441 tgtagatatc gatgtaatca atacccagac gttgcaagct ggcttctacc tcttcgcgga
    46501 tagattccgg agaaaggttt ttatacaact gccgatcgcc aactttgttg aataaacttc
    46561 cttttcgttc ccagacaatg ccgcatttgg tttctactac aacctgttca cggggcagtt
    46621 tttttaacgc ctgaccaacg ataacttcac tattgccaaa gttatatcct ggcgcagtat
    46681 caatcaggtt aatgccgcaa cgatgggctt caagaatcgt atcaatgcat atttgtcgat
    46741 cgagatcgcc attccatgca ggaccgccgc caatagccca tgtacccaac cccattcgcg
    46801 aaagcgttat atccgctgtg cctaaaggta tctttttcat ttgccttcat tatccttcgt
    46861 attcttccag caattgttcg accagctttc tgttttttac accggtggtg gcgccgatgc
    46921 ttagaaccga gatagccgcc gttgcattgg caaagcgtgc gcattctcgc agatttttgc
    46981 cttctaacag tgctgcaata aaacctgaag caaagttatc gcccgcacca atggtgtcga
    47041 tagcggttat tcctgcgacc gccggcacct tcatcgtcat gtcaccacgc ttgataaagc
    47101 agccgtcttt acccgtttta atcaccaccg tttttacgcc gcacgcaaga aagcagtcag
    47161 caatttcatc cagtgtctct ttcccggtga gtaattttgc ctcggcaaaa ttaggaaaca
    47221 gataatcgac ataactcaat gcttcgcaaa tatcattcag cgtttcattc aaccgcggtt
    47281 tgatcatatc ggcacagata atcatctgcc gggttttagc ttgcgtaaaa atttctgtta
    47341 gcgctcgacc atccaataga ggactgttga aaatactggc cagtgataat aatttcgcct
    47401 gagaaaaccg cgcaaaatca acatcgtcaa tattcaattt ccacagactg ccgttacggt
    47461 ttgtgacaaa ggtccgctcg ccatcctccg tcaccagtcc aacgttaata gaggtatcta
    47521 tgctgacgtc ctgcttcagg ctttgaatat caatattctc tttgcggcaa tggtcgagaa
    47581 taaattgccc tgcggcatct ttaccaatac gactcattaa tgctgtgcga tggcccagac
    47641 gagaaataat tgttgcttca tttattgcat cacccccggt ggtcattgcg attctttcaa
    47701 gaggataaga atccacatca aagatatttt tactgaccgg ttgcaatgga atatcaacaa
    47761 tagcggcacc tatacaaata acgtcgagat tatccatatt attattccgc tttgccatca
    47821 gaaccgaaca gtttaatttt atccaatgcc cgttctttta tagctttacg cacttcacgt
    47881 tctaaatgca ggaaaggctg atcctggttt tctttcactg ctaccattgc ggcctgacag
    47941 agctccgtat gaatgttgat tttcgcaata cccaacgaaa ttgcagtttt aatgtcggca
    48001 tcactgatcc ctgatgcgcc atgcaaaacc agcggaacag aaacagcatc gcgtacgcgt
    48061 ttgacgacct caaagttcaa ttgtggctct gacgtataaa ccccatgctg gttgccgatg
    48121 gcgacagcca atgagtcaca gcccgtacgt tcaacaaatt cagccgcctg gtcaggatcg
    48181 gtatagtgat agcctgccag cgcttcctca taaacggttt cattaccgac atgccctaat
    48241 tccgcctcta ccggaatacc cagcggatgg aagaaatcaa cagcctcttt ggttaaacga
    48301 atattttctt cgaaatcaaa tgcagaagca tcacgcatta atgaattcat accatgagtc
    48361 caggcgttat gaataatctc catactccga ccatgatccc aatgagttat taccggcacc
    48421 gttgcttttt gtgccattga taccatcatg tgagagaaat cttcaaatga ggtgttgccg
    48481 acaaaacctg tgccaaaaga aataataacc ggtgatttcg cttcttcggc tgcgtcgata
    48541 acgcccatca acatttctgc attccatacg ttaaaatggg caattgcata atgtttattt
    48601 gtggcatcgt tttcccaata tctgatatct gcgagcatgt tctgttctcc cgtattaatt
    48661 aatcgctaat tttaataacg cctttaataa tgtcgcgttt gttgttaact gactcttcaa
    48721 atgcctgttg tacatcccga taatcgtaaa tatgcgtcac catcgatttc acatcgaatc
    48781 gccctgaaga aatagcttca atcgtgaccg gataacgatt ggcatagcgg aataccgtct
    48841 ggatagtgac ttcgcgattg attttgagga aattgattgc cgaatcgccg ggtacagtac
    48901 caacaatcat aattttaccg ccgcgcatga ccagataagg tgcctgttta acggtgaccg
    48961 cagaacccgc tgtttcaaaa acaatatctg cgcccatgtc tttggtaaat tgctgacagc
    49021 gtgcaatagt gtcttctttt gcgccgttaa taaccactgt cgcgccgagc tgttccgcca
    49081 ttgtcagacg tttttccagc acatcaacga cggcaatttc cgttgctccc aggcatttgc
    49141 acgcttgcaa cgtcatcaaa ccaatacaac ccgctcccag aataattatc ttcttacccg
    49201 gtttaacatc tgccagcatc gcggcatgca tcccgactgc ggcaggctcc accagcgccc
    49261 cttccatcgt gtccatattg tctggcagtt tgtaagtaaa gctctccgga tgacacagat
    49321 agtgcgttaa tgcgccgcgg tagttgggtt gtgtcgccat aaaatcaacg tccgggcaga
    49381 tgttatattt gccttccaga cagtaacgac agtgaccgca aggaacgcca ggttcgatat
    49441 ttacccgatc ccccggttta aatttgcgca cgcggcttcc cacagccaca accgtcccgg
    49501 cgcattcatg acccaggcca atttcttgat ttgggtcttt aggaggaata aacgggcctg
    49561 attcaaaacc atgtacatct gaaccacaaa taccgacata ttccacttta atcaaaactt
    49621 catcttcttt aggtacaggt atttctgctg aaataatttt cattgtgccc ggcacctgca
    49681 acattgcttt tgaatttttc attttgaatc caccagtatt atttattggc tactgcatca
    49741 atacttaaac tttcaacgga gacacctttg gtttcaattc caatggtggc gatagcaatt
    49801 gcgacaataa ttgaaacagc ccccagaaga ataaagactc ccgttacgcc ataactactg
    49861 agcagcactg caacggcata aggtgcggca ataccactga ttcgccctac cgcattcgcc
    49921 agaccggagc cacggagttt ggcctctgtc ggccagatct cagggacata cactgccgag
    49981 gcatagcaaa cgtacatata gacgaaagta atcaggaaga aaccaatcag cgttatgagc
    50041 aacatactgg tttgcagtga atagatatat ccgagcaccg caatcaggat taatagcccc
    50101 acacccattg ttttacgcgg aattttatcc atcaccagca tggcaataaa aataccaaat
    50161 ggcgcgccaa acatactcat ggtatttaaa acaatcgagt cttttaaatt aattccctgg
    50221 gtcatgaata tcgttggcaa ccagttaatt agtgtgtact gcacaacgtt catggcaatc
    50281 agcacacaag agcctaatat cacgcgtttc agtaatactc ctgtcagtaa ggctgaatac
    50341 ggcaccgctt gtggtgcttt accgtcatca gcaataacca caggcggcaa aggtttaccc
    50401 gtctggcgta tgacgccttc ttctatactc cgcatgactt tctcggcttc ctgataacgt
    50461 ccgcgcgatt ccagccagcg cggggattca ggaaagtagc gccaggccag cgccgtagcg
    50521 ataagtgaca atattgcagg gataagcagt tgtacccgcc agttccactc tgcactaatc
    50581 agcggcgtga gtcccatcgc aatcaatgaa cagagcggat atgaccagtt accaataaag
    50641 gaaacccgac tggaccacgt tccgcgattt cttccgggca tatattcggt gaaaccagca
    50701 aacagtgtaa ccagtaaagc tcccagccca acgcccatca caaaacggca ggcaataagg
    50761 aaatccatat tcggtgagaa agcaccgaca accatcgagg caatatgaat ggcctcatag
    50821 aggataaacg catttctgcg accggttttg tcaccgatga tgccgcccac cagcgcaccg
    50881 agaaacattc cggcggtcgt gattgctgag aatgtggcag tggtggaatt atctgtccag
    50941 cccaacgctt tcagctgcgc gaggatcaag ccaccaacgg cattactcca gcagacaagc
    51001 aggccaaacg cgacgatggc aaacatcgac gaatgccagc ggcaatccgg taagcgatcc
    51061 agccgtgcac cacaatgcgg ttttgttatc tgttccatgc gataacgttc cttcaggaga
    51121 gtagttattc atcaaagtcg taagtcatga tcactttgat tgcagtttta tcgaccatcg
    51181 catcaaaccc ttcgcgccat tgcgacaggc cgatacgatg cgtgatcatc ggtttgactt
    51241 tgatagcgcc gctggctaat agcctgatag cgttacgcca tgaagtggag tcataggcca
    51301 tatgcccaat gatgcttttg ttccaggcgg taatgtcatt aatcgagaaa tcaagaggtt
    51361 tgaagcccat tccaacgcgt actacttccc cattcgggcg gagcatttcg atggcttgtt
    51421 tcaatgcgat attggcaccg gagcattcaa tcaccagccc cagattgtct ttgccacaaa
    51481 tttgctggca gcgtgccacc acatcttcgg tagaaccatt tactactgcc gtcgcaccca
    51541 gttcttttgc aaccgggaag cggaccgtta catcttcttg cagaccaacg acgacgatat
    51601 ttaccgcccc cataatccgc gccatttgta cggagaacag cccgagtggg ccagtgccga
    51661 tgacgaccac atcctgacct ggaaggaatt tcgattgctg cgcgatggat ttataggcat
    51721 tacagatagg gtcaagtacg gctgcgtcct cataatcaac accatcaggg atttcccaca
    51781 acgcatgacg atgaattttg agaatttcac caggaacaag acaatatttg gaaaaaccac
    51841 cgccccaggt attgttatcc agaccaaggt ttactttttc tgtacaacac agaaaatcac
    51901 cttgttcaca ggccgggcaa acgccgcaaa cgtgaccgct gttatccgac acgacgcgtt
    51961 gccccacttt ccagtctttg actttttcac caacctgcgc aatacaacct gcgaactcat
    52021 ggccgcggat agagttaaac tcatccgaac cgctatcgac attgtagtgc ttcatgtctg
    52081 cgccgcagat tgccgcggct ttaatttcaa tcacgacatc ttccgggcca caaatgggtt
    52141 gtgggacatc aatcatcttg tagccaccaa aagccttgcc aaaccgagcc agtgctttca
    52201 ttgcgtcatc ctctgtgttt tgttttgctt agatggtcat catacttacc cacatgattt
    52261 ttgaggtcaa cattaaatct gtaaaacaca gataatgatc tgcgttttac aacttagatc
    52321 acaattacgc agaaaatggc tggggttgtt acaggttgat ggaaggcggg acgcaaaaag
    52381 agcaaattcg agagggattc ccggccaaat aggcctgaga cttaagttca ggagaagtca
    52441 ttcaggtgtg aacgcgactg caagagatcg gactgggact tagccaggtc ggggcgagat
    52501 aattgagatg agtcgtgggg tcattgttgg ctatcccctt tataaccgcg gcaaaaaggg
    52561 gataaaccac ttacttaaac atggcaatag gctttgctga gataccaaaa tcttctttta
    52621 actgctgttt gctcttcatg accatctgac catttgtgtc aattgtcata tgctgtgctt
    52681 cggtattatg gcgggcttgc cacagcatca ccagttgcag gcagttctct ttttgttcct
    52741 cggtcaacgt aacgccatca ggccatttcc ccagttcaac ggcggtcgac aaacgctggt
    52801 atacctcagg cgtcatgctg ttgataatgt catcaagatt cataattttt ccgctcctgt
    52861 ggaataagtt gctgaatcgt tttttcaacc gttgatttct tcgccgtttt cgccatcagt
    52921 gaagcgtaaa gaagcagagt taacacaata acgttcgccc gttggctgcg gcccgtcagg
    52981 aaagacatgg cccagatggg catcgcagtt accgcaacga atttctatgc gctgcattcc
    53041 atgtgacaat tctttgatat aacgaataga ttcttcactc accggttcgt agaaactggg
    53101 ccagccacag ccggaatcat acttggtttg ggaatgaaac agcggggcat cgcagatcaa
    53161 acagtgatat acgccgtcac gcttgttatg cagtaaacga cccgtaaatg gcggttctgt
    53221 cccatgattc tgcgtcacgt agaactgcat ctcggacaaa ttttttttca gttcttctgc
    53281 cgaaggttta ttagccattt gctcacatct cactttaatc gtgctcacat tacgtgactg
    53341 attctaacaa aacactaaca ccaactggca aaattttgtc ctaaacttga tctcgacgaa
    53401 atggctgcac ctaaatcgtg atgaaaatca catttttatc gtaattgccc tttaaaattc
    53461 ggggcgccga ccccatgtgg tctcaagccc aaaggaagag tgaggcgagt cagtcgcgta
    53521 atgcttaggc acaggattga tttgtcgcaa tgattgacac gattccgctt gacgctgcgt
    53581 aaggtttttg taattttaca ggcaaccttt tattcactaa caaatagctg gtggaatata
    53641 tgactatcaa agtaggtatc aacggttttg gccgtatcgg tcgcattgtt ttccgtgctg
    53701 ctcagaaacg ttctgacatc gagatcgttg caatcaacga cctgttagac gctgattaca
    53761 tggcatacat gctgaaatat gactccactc acggccgttt cgacggtacc gttgaagtga
    53821 aagacggtca tctgatcgtt aacggtaaaa aaatccgtgt taccgctgaa cgtgatccgg
    53881 ctaacctgaa atgggacgaa gttggtgttg acgttgtcgc tgaagcaact ggtctgttcc
    53941 tgactgacga aactgctcgt aaacacatca ccgctggtgc gaagaaagtg gttatgactg
    54001 gtccgtctaa agacaacact ccgatgttcg ttaaaggcgc taacttcgac aaatatgctg
    54061 gccaggacat cgtttccaac gcatcctgca ccaccaactg cctggctccg ctggctaaag
    54121 ttatcaacga taacttcggc atcatcgaag gtctgatgac caccgttcac gctactaccg
    54181 ctactcagaa aaccgttgat ggcccgtctc acaaagactg gcgcggcggc cgcggcgctt
    54241 cccagaacat catcccgtct tctaccggtg ctgctaaagc tgtaggtaaa gtactgccag
    54301 aactgaatgg caaactgact ggtatggcgt tccgcgttcc gaccccgaac gtatccgtag
    54361 ttgacctgac cgttcgtctg gaaaaagctg caacttacga gcagatcaaa gctgccgtta
    54421 aagctgctgc tgaaggcgaa atgaaaggcg ttctgggcta caccgaagat gacgtagtat
    54481 ctaccgattt caacggcgaa gtttgcactt ccgtgttcga tgctaaagcg ggtatcgctc
    54541 tgaacgacaa cttcgtgaaa ctggtatcct ggtacgacaa cgaaaccggt tactccaaca
    54601 aagttctgga cctgatcgct cacatctcca aataagttga gatgacactg tgatctaaaa
    54661 agagcgactt cggtcgctct tttttttacc tgataaaatg aagttaaagg actgcgtcat
    54721 gattaagaaa atttttgccc ttccggtcat cgaacaaatc tcccctgtcc tctcccgtcg
    54781 taaactggat gaactggacc tcattgtggt cgatcatccc caggtaaaag cctcttttgc
    54841 attacagggc gcacaccttc tctcgtggaa acctgcgggt gaagaagaag ttctgtggtt
    54901 gagtaacaac acaccgttca aaaatggcgt cgccattcgc ggtggcatac cggtttgctg
    54961 gccgtggttt ggtccggctg cacaacaagg tctgcctgcg cacggttttg cccgcaacct
    55021 gccgtggacg ctgaaatcac atcgtgaaga tgctgatggt gtagcgctga cttttgaatt
    55081 gacacaaagc gaagagacga aaaaattctg gccgcacgac tttacgctgt tagcgcattt
    55141 ccacgtgggt aaaacctgtg aaatcgatct tgaagcacac ggcgaatttg aaactacctc
    55201 tgctctgcat acctacttta acgtgggtga tatcgctaag gtaagcgtca gtggactagg
    55261 cgatcgcttc attgataaag taaatgacgc gaaagaagat gtactgaccg acggtattca
    55321 gaccttccct gaccgtaccg atcgcgtgta tctgaatccg caagattgca gcgtaattaa
    55381 tgacgaagcg ctgaatcgta ttatcgccgt aggccaccag catcatctga acgttgtagg
    55441 ctggaaccca ggaccggcgc tttcagttag catgggcgat atgccggatg atggctacaa
    55501 gacctttgtt tgtgtagaaa cggcttacgc ttcagaaacg caaaaagtca ccaaagagaa
    55561 acctgcacat ctggcgcaat ccattcgcgt tgctaaacgt taatttgcgt taatattgtg
    55621 tgccgggtgc gatgcatccg gcacgaatca acatcacacc atatccagcg cagtttttcc
    55681 ttttggtgcc ggatacgctt tatccagcat aactaattcc gctgacgaaa gttcaacctc
    55741 aagcacagcc gcattttgtt ggacatgtgc gacagttgcc gcttttggaa tcgccatcac
    55801 accctgatgg ctgattaccc acgccaacaa tacttgtgcc gcactgatat tgtgagcatg
    55861 tgcaatttcg ttgactaccg cattttttaa cagtccattg cgcaaccgcc cggcctgggc
    55921 taatggactg taagccatca ccggcatctg ctgtttctgg caccagggaa gtagatcgta
    55981 ctcaatccct cgtgaaccga gatggtaaag cacctgatta gtggcacact gatttccccc
    56041 cggcagctgc cagagttcct gcatatcggc ataatcaagg ttagaaacgc cccagcggcg
    56101 gatttttccc tgggcgatca atttttccat cgctgcgaca gtctcttcga gagcgaaact
    56161 gccagaccag tgtaataagt agagatcgag ataatcagta ttgagacggc gtaaactggc
    56221 ttcgcatgca tttatcgctt tttgcccgcc agcattccac ggatagactt tagagacgag
    56281 aaagacgttc tcacgcagac cgattaatgc ttccccaacc actttttcgg caccgccatc
    56341 ggcatacatt tcggcggtat caatgagggt taaaccgagt tcaatgcccg cgcgtagtgc
    56401 agcaacttct gttttacgct gactggcatc ttcgcccata taccatgttc cctgccctat
    56461 ggctggcagt gagacatcgc cactaaattg aatcattttt tgttgcattg tttcctccca
    56521 ggtattgcac caccgtaatg caaaacaggg cattacgccc tgtttttatg cacaaaatgc
    56581 cctggaaaga tgcattatca gaatttgtag gtgatcccgg tagaaatcag gccagtccag
    56641 gatttatcca ccattgggct gtcagtaact tcatcagaca gacgggtgta gcgcgcggta
    56701 ccgtaaacac tccagtcgcc gaggaagttg tagctggcgc tcagctccag gtaagggctc
    56761 cagctgtcgt tcgggttata gccacgcaga ccgctgcgag cggactcttt gcgcgatacg
    56821 ccataatagt attcgttctg gttttcgctg ttccactgca caccaatacc cggagtcacg
    56881 gtcaggccac cgttggtgta acgatacaac caggccatat cccagacgat gccgttgctg
    56941 ttatccaggg tatcgccagc cagggtggta cgcaggtaac cgtactgggt aaagtgagca
    57001 taagacagac cagccatcat ggtgctctta cggtcatcga ggtgacgcat ttggtgatcg
    57061 ccactgtctt tagctttgaa gtaaagcggc gaccagtaag cggtaattga aagtttgtcc
    57121 gtcgcgtcat tccacaggta gtaaccacca cctaagccac ggaaccagaa gttatcgcct
    57181 tcatagttga ttaccggtac tgggtaaaca tcggtatcgt aatctttata tgggtgttca
    57241 acgacaccta cgcctgcgcc cagggaaaat ttaccttcag cgtgcgctac gcctgcagac
    57301 gttgcgataa gcactccaag tgccagaagt ttgagtttgg tcacaattaa tcattcctta
    57361 aacaaatgtt tagcgggcga caaagtttac ccgtcaatac atcgaaaccc aaccttttta
    57421 cgttttcatt ttttatagta actgtttaat tttcctgacg cagatgacac cacgcttaca
    57481 gccaaatgaa tttagcgtta ccggcgaccc tattatttac attaattatg caaaatttat
    57541 ggatgagttg ttgatatgcc attgaaatta agaaagccgt gcaggcaagt tttccatttg
    57601 ccatctacgc ttaattttga aggtgtatca ccgggcacgt tgttctcatc gtcaataaaa
    57661 tggcatgaga gttgctgtgt tttagcaaga gacgtcgttc agtttacctc ttccgggagc
    57721 ctctactatt catatgaacg gctcttaacc tgtgctaaaa aacgaaagga cggcatacca
    57781 tgaatatatt cgatcactat cgccagcgat atgaagctgc caaggacgaa gagttcacac
    57841 tgcaggagtt tcttaccact tgtcggcaag atcgcagtgc ttatgccaac gcggctgagc
    57901 ggctattgat ggctattggt gagcctgtca tggtcgatac agcccaggaa cccagacttt
    57961 ctcgactctt ttctaaccgg gtcattgcac gttatccggc gtttgaagag ttttacggca
    58021 tggaagacgc gattgaacag attgtttctt atctgaaaca cgcggctcag gggctggaag
    58081 agaagaaaca aattctgtat ctgctggggc ctgtgggtgg gggtaaatca tcacttgctg
    58141 agcgactgaa atcattaatg cagctcgtac cgatttatgt attgagcgcc aacggtgagc
    58201 gtagcccggt caacgatcat ccgttctgtc ttttcaatcc gcaggaagat gcgcagattc
    58261 tggaaaaaga gtacggcatt cctcgccgtt atctcggcac catcatgtcg ccgtgggcgg
    58321 caaagcgcct gcatgaattt ggtggcgata tcactaagtt ccgggtagtg aaggtctggc
    58381 cgtcaattct gcaacaaatt gccatcgcca aaacggaacc cggagatgag aacaaccagg
    58441 acatctccgc cctggtaggg aaagtcgata ttcgtaaact cgaacactac gcgcagaatg
    58501 acccggacgc ctacggctat tccggtgcgc tgtgccgcgc caatcagggg atcatggaat
    58561 ttgtcgagat gtttaaagca ccgataaaag tgctgcatcc cttgttaacc gccacccagg
    58621 aaggtaacta caacgggacg gaaggtatct ccgccctgcc gttcaacggg attattctcg
    58681 cccactcgaa cgaatccgaa tgggtcactt tccgtaataa caaaaacaac gaagccttcc
    58741 tcgaccgtgt ttacatcgtg aaggtgccgt attgcttgcg catttccgaa gagatcaaaa
    58801 tctacgagaa attgcttaat cacagtgaat tgactcacgc cccctgcgcc cctggcacgc
    58861 tggaaacgct gtcacgtttt tctattcttt cgcgtctgaa agagccagaa aactccagca
    58921 tttattcaaa gatgcgcgtt tatgatggtg aaagtctgaa agacactgat cccaaagcca
    58981 agtcgtatca ggaatatcgt gactacgccg gtgtcgatga agggatgaac ggtctgtcga
    59041 cgcgttttgc gtttaagatc ctctcccgcg tgttcaactt cgatcatgta gaagtggcgg
    59101 caaacccggt ccatctgttc tacgtcctgg aacagcagat cgagcgcgag cagttcccgc
    59161 aagagcaggc tgaacgctat ctggagttcc tgaaaggtta tctgatcccg aaatatgccg
    59221 agtttatcgg caaagagatc cagacggcct accttgaatc ctattcagaa tatgggcaaa
    59281 acattttcga ccgttatgtt acctacgcgg atttctggat tcaggatcag gagtatcgcg
    59341 atccggatac cgggcagctg tttgaccgcg agtctcttaa cgccgagctg gagaaaatcg
    59401 agaaaccggc ggggatcagt aatccaaaag atttccgcaa cgagattgtt aacttcgtac
    59461 tgcgagccag agcgaataac agcggacgca atccgaactg gaccagctat gaaaaactgc
    59521 gcacggttat cgagaagaaa atgttctcca ataccgaaga gctgttgccg gttatttcgt
    59581 ttaacgccaa aacgtcaacc gacgagcaga agaaacacga cgactttgtc gaccgtatga
    59641 tggaaaaagg ctacacccgt aaacaggtgc gtttactgtg cgaatggtat ttgcgcgtac
    59701 gtaaatcgtc ttaacaaccc tggcccggta cacatgctac cgggcctaca acgacagcga
    59761 accgtgggcc agagaagcgg caacacaagc gtagcatacc gttggcaaat gtagtacggg
    59821 gggcatatga cctggtttat tgaccggcgt ctgaacggca aaaataaaag catggtgaat
    59881 cgccagcgtt tcttacgccg ttataaagcg caaattaaac agtcgatctc cgaggccatt
    59941 aataagcgtt cggtgactga cgtcgacagc ggcgagtccg tatccattcc cacggaagat
    60001 attagcgaac cgatgtttca tcaggggcgt gggggcctgc gccaccgtgt gcatccgggc
    60061 aatgaccatt tcgtccagaa cgaccgaatt gaacgtcccc agggtggtgg cggaggttcc
    60121 ggcagtggtc agggccaggc cagccaggat ggtgaaggtc aggatgaatt tgtctttcag
    60181 atttcgaaag atgagtatct tgatctgctc tttgaagatt tggccttacc gaatctgaaa
    60241 caaaaccaac aacgccagct gaccgaatat aaaacgcatc gggcgggtta taccgctaac
    60301 ggcgttccgg ccaatatcag cgttgtacgt tcattgcaga actcactggc gcggcgcaca
    60361 gctatgacgg cgggcaagcg gcgggaactt catgcgctgg aagagaattt ggccatcatc
    60421 agcaacagtg aacctgcgca actgctggaa gaggaacgtc tgcgcaaaga aattgcagaa
    60481 ttacgtgcca aaattgaacg cgtccctttc attgacacct tcgatttacg ttacaagaac
    60541 tacgaaaaac ggcccgatcc ctccagccag gcagtgatgt tttgcctgat ggacgtttcc
    60601 ggttcaatgg atcagtccac taaagatatg gctaaacgtt tttatattct gctgtatctg
    60661 ttcctcagca gaacgtataa gaacgtggaa gtcgtataca tccgccatca tacccaggcg
    60721 aaagaagtcg atgaacatga gtttttctac tcgcaggaaa ctggcggcac cattgtttcc
    60781 agcgccctaa aactgatgga tgaggtagtg aaagagcgtt ataacccggc acagtggaat
    60841 atttacgcag cccaagcatc agacggcgat aactgggccg atgactctcc gctttgccat
    60901 gaaattctgg cgaaaaaaat attacccgtt gttcgttatt acagctatat cgaaattaca
    60961 cgtcgcgcgc atcagacact gtggcgagaa tatgagcatc tgcaatctac tttcgacaac
    61021 tttgcgatgc agcatatccg cgaccaggat gatatttatc cggtgttccg tgaactgttt
    61081 cataaacaaa atgcaacagc taaagactaa aactgtcagc cgggtcatta tcgcctggct
//
LOCUS       DAJOTB010000008        61135 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJOTB010000008 DAJOTB010000000
VERSION     DAJOTB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.008"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            17..988
                     /locus_tag="OHN28_000538"
     CDS             17..988
                     /locus_tag="OHN28_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCQ2295984.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDIPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQAPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQYD
                     IPAVMITDTAFYRNKQYHFPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1091..1594
                     /locus_tag="OHN28_000539"
     CDS             1091..1594
                     /locus_tag="OHN28_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCQ2295985.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1661..3118)
                     /gene="nuoN"
                     /locus_tag="OHN28_000540"
     CDS             complement(1661..3118)
                     /gene="nuoN"
                     /locus_tag="OHN28_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCQ2295986.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3125..4654)
                     /gene="nuoM"
                     /locus_tag="OHN28_000541"
     CDS             complement(3125..4654)
                     /gene="nuoM"
                     /locus_tag="OHN28_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCQ2295987.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4818..6659)
                     /gene="nuoL"
                     /locus_tag="OHN28_000542"
     CDS             complement(4818..6659)
                     /gene="nuoL"
                     /locus_tag="OHN28_000542"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCQ2295988.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6656..6958)
                     /gene="nuoK"
                     /locus_tag="OHN28_000543"
     CDS             complement(6656..6958)
                     /gene="nuoK"
                     /locus_tag="OHN28_000543"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCQ2295989.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6955..7509)
                     /gene="nuoJ"
                     /locus_tag="OHN28_000544"
     CDS             complement(6955..7509)
                     /gene="nuoJ"
                     /locus_tag="OHN28_000544"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCQ2295990.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7521..8063)
                     /gene="nuoI"
                     /locus_tag="OHN28_000545"
     CDS             complement(7521..8063)
                     /gene="nuoI"
                     /locus_tag="OHN28_000545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCQ2295991.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8078..9055)
                     /gene="nuoH"
                     /locus_tag="OHN28_000546"
     CDS             complement(8078..9055)
                     /gene="nuoH"
                     /locus_tag="OHN28_000546"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCQ2295992.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9052..11778)
                     /gene="nuoG"
                     /locus_tag="OHN28_000547"
     CDS             complement(9052..11778)
                     /gene="nuoG"
                     /locus_tag="OHN28_000547"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCQ2295993.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11831..13168)
                     /gene="nuoF"
                     /locus_tag="OHN28_000548"
     CDS             complement(11831..13168)
                     /gene="nuoF"
                     /locus_tag="OHN28_000548"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCQ2295994.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13165..13665)
                     /gene="nuoE"
                     /locus_tag="OHN28_000549"
     CDS             complement(13165..13665)
                     /gene="nuoE"
                     /locus_tag="OHN28_000549"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCQ2295995.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13668..15470)
                     /gene="nuoC"
                     /locus_tag="OHN28_000550"
     CDS             complement(13668..15470)
                     /gene="nuoC"
                     /locus_tag="OHN28_000550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCQ2295996.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15564..16226)
                     /gene="nuoB"
                     /locus_tag="OHN28_000551"
     CDS             complement(15564..16226)
                     /gene="nuoB"
                     /locus_tag="OHN28_000551"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCQ2295997.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16242..16685)
                     /gene="nuoA"
                     /locus_tag="OHN28_000552"
     CDS             complement(16242..16685)
                     /gene="nuoA"
                     /locus_tag="OHN28_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCQ2295998.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(17315..18253)
                     /gene="lrhA"
                     /locus_tag="OHN28_000553"
     CDS             complement(17315..18253)
                     /gene="lrhA"
                     /locus_tag="OHN28_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCQ2295999.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19173..20390
                     /gene="alaA"
                     /locus_tag="OHN28_000554"
     CDS             19173..20390
                     /gene="alaA"
                     /locus_tag="OHN28_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCQ2296000.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20474..21073
                     /gene="yfbR"
                     /locus_tag="OHN28_000555"
     CDS             20474..21073
                     /gene="yfbR"
                     /locus_tag="OHN28_000555"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCQ2296001.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21132..22964)
                     /locus_tag="OHN28_000556"
     CDS             complement(21132..22964)
                     /locus_tag="OHN28_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCQ2296002.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23051..23701)
                     /gene="hxpA"
                     /locus_tag="OHN28_000557"
     CDS             complement(23051..23701)
                     /gene="hxpA"
                     /locus_tag="OHN28_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCQ2296003.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23712..24206)
                     /locus_tag="OHN28_000558"
     CDS             complement(23712..24206)
                     /locus_tag="OHN28_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCQ2296004.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24289..24744)
                     /locus_tag="OHN28_000559"
     CDS             complement(24289..24744)
                     /locus_tag="OHN28_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCQ2296005.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25082..26284
                     /gene="ackA"
                     /locus_tag="OHN28_000560"
     CDS             25082..26284
                     /gene="ackA"
                     /locus_tag="OHN28_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCQ2296006.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26359..28503
                     /gene="pta"
                     /locus_tag="OHN28_000561"
     CDS             26359..28503
                     /gene="pta"
                     /locus_tag="OHN28_000561"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2296007.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28693..30213
                     /gene="yfcC"
                     /locus_tag="OHN28_000562"
     CDS             28693..30213
                     /gene="yfcC"
                     /locus_tag="OHN28_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCQ2296008.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGRAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30246..30788)
                     /gene="yfcD"
                     /locus_tag="OHN28_000563"
     CDS             complement(30246..30788)
                     /gene="yfcD"
                     /locus_tag="OHN28_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCQ2296009.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30846..31397)
                     /gene="yfcE"
                     /locus_tag="OHN28_000564"
     CDS             complement(30846..31397)
                     /gene="yfcE"
                     /locus_tag="OHN28_000564"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCQ2296010.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31453..32097)
                     /gene="yfcF"
                     /locus_tag="OHN28_000565"
     CDS             complement(31453..32097)
                     /gene="yfcF"
                     /locus_tag="OHN28_000565"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCQ2296011.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32233..32880
                     /gene="yfcG"
                     /locus_tag="OHN28_000566"
     CDS             32233..32880
                     /gene="yfcG"
                     /locus_tag="OHN28_000566"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCQ2296012.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            32937..33299
                     /gene="folX"
                     /locus_tag="OHN28_000567"
     CDS             32937..33299
                     /gene="folX"
                     /locus_tag="OHN28_000567"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCQ2296013.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            33320..34213
                     /locus_tag="OHN28_000568"
     CDS             33320..34213
                     /locus_tag="OHN28_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCQ2296014.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34372..35145)
                     /gene="hisP"
                     /locus_tag="OHN28_000569"
     CDS             complement(34372..35145)
                     /gene="hisP"
                     /locus_tag="OHN28_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCQ2296015.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35153..35869)
                     /locus_tag="OHN28_000570"
     CDS             complement(35153..35869)
                     /locus_tag="OHN28_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2296016.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35866..36552)
                     /gene="hisQ"
                     /locus_tag="OHN28_000571"
     CDS             complement(35866..36552)
                     /gene="hisQ"
                     /locus_tag="OHN28_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCQ2296017.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36642..37424)
                     /gene="hisJ"
                     /locus_tag="OHN28_000572"
     CDS             complement(36642..37424)
                     /gene="hisJ"
                     /locus_tag="OHN28_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCQ2296018.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37645..38427)
                     /gene="argT"
                     /locus_tag="OHN28_000573"
     CDS             complement(37645..38427)
                     /gene="argT"
                     /locus_tag="OHN28_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCQ2296019.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(38693..39262)
                     /gene="ubiX"
                     /locus_tag="OHN28_000574"
     CDS             complement(38693..39262)
                     /gene="ubiX"
                     /locus_tag="OHN28_000574"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCQ2296020.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(39357..40874)
                     /gene="purF"
                     /locus_tag="OHN28_000575"
     CDS             complement(39357..40874)
                     /gene="purF"
                     /locus_tag="OHN28_000575"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCQ2296021.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(40911..41399)
                     /gene="cvpA"
                     /locus_tag="OHN28_000576"
     CDS             complement(40911..41399)
                     /gene="cvpA"
                     /locus_tag="OHN28_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCQ2296022.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41840..42502)
                     /gene="dedD"
                     /locus_tag="OHN28_000577"
     CDS             complement(41840..42502)
                     /gene="dedD"
                     /locus_tag="OHN28_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCQ2296023.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42492..43760)
                     /gene="folC"
                     /locus_tag="OHN28_000578"
     CDS             complement(42492..43760)
                     /gene="folC"
                     /locus_tag="OHN28_000578"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCQ2296024.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43830..44744)
                     /gene="accD"
                     /locus_tag="OHN28_000579"
     CDS             complement(43830..44744)
                     /gene="accD"
                     /locus_tag="OHN28_000579"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCQ2296025.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44900..45559)
                     /locus_tag="OHN28_000580"
     CDS             complement(44900..45559)
                     /locus_tag="OHN28_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2296026.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(45642..46454)
                     /gene="truA"
                     /locus_tag="OHN28_000581"
     CDS             complement(45642..46454)
                     /gene="truA"
                     /locus_tag="OHN28_000581"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCQ2296027.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46454..47467)
                     /locus_tag="OHN28_000582"
     CDS             complement(46454..47467)
                     /locus_tag="OHN28_000582"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2296028.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALCPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47533..48669)
                     /gene="pdxB"
                     /locus_tag="OHN28_000583"
     CDS             complement(47533..48669)
                     /gene="pdxB"
                     /locus_tag="OHN28_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCQ2296029.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48768..49763
                     /gene="flk"
                     /locus_tag="OHN28_000584"
     CDS             48768..49763
                     /gene="flk"
                     /locus_tag="OHN28_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCQ2296030.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49760..50938)
                     /locus_tag="OHN28_000585"
     CDS             complement(49760..50938)
                     /locus_tag="OHN28_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HCQ2296031.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51203..52423)
                     /gene="fabB"
                     /locus_tag="OHN28_000586"
     CDS             complement(51203..52423)
                     /gene="fabB"
                     /locus_tag="OHN28_000586"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCQ2296032.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52582..54588
                     /gene="mnmC"
                     /locus_tag="OHN28_000587"
     CDS             52582..54588
                     /gene="mnmC"
                     /locus_tag="OHN28_000587"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCQ2296033.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54709..54987)
                     /locus_tag="OHN28_000588"
     CDS             complement(54709..54987)
                     /locus_tag="OHN28_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCQ2296034.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(55021..55569)
                     /gene="epmC"
                     /locus_tag="OHN28_000589"
     CDS             complement(55021..55569)
                     /gene="epmC"
                     /locus_tag="OHN28_000589"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCQ2296035.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55569..56378)
                     /locus_tag="OHN28_000590"
     CDS             complement(55569..56378)
                     /locus_tag="OHN28_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCQ2296036.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56378..57202)
                     /gene="mepA"
                     /locus_tag="OHN28_000591"
     CDS             complement(56378..57202)
                     /gene="mepA"
                     /locus_tag="OHN28_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCQ2296037.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57206..58291)
                     /gene="aroC"
                     /locus_tag="OHN28_000592"
     CDS             complement(57206..58291)
                     /gene="aroC"
                     /locus_tag="OHN28_000592"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCQ2296038.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(58326..59258)
                     /gene="prmB"
                     /locus_tag="OHN28_000593"
     CDS             complement(58326..59258)
                     /gene="prmB"
                     /locus_tag="OHN28_000593"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2296039.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59424..59975
                     /gene="smrB"
                     /locus_tag="OHN28_000594"
     CDS             59424..59975
                     /gene="smrB"
                     /locus_tag="OHN28_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCQ2296040.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60035..60859)
                     /locus_tag="OHN28_000595"
     CDS             complement(60035..60859)
                     /locus_tag="OHN28_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCQ2296041.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(60861..>61135)
                     /locus_tag="OHN28_000596"
     CDS             complement(60861..>61135)
                     /locus_tag="OHN28_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2296042.1"
                     /translation="EVRVTLTGTEDSEQPGFLALDAASTAQGVGIGMEKTDGTAVLIN
                     NTSGATFVLSSGNNDLHFNAWLQAKSGRDVTMGDFTASLTATFEYI"
BASE COUNT        15143 a        16697 c        15402 g        13893 t
ORIGIN      
        1 cattaataat ttgcatatga aaaaaataaa ttttgctttt attatattat ttctattttc
       61 actccctctg attatttttt accaaccctg ggtgaatgca ttaccaccaa cgccccgaca
      121 cgctagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      181 tagcgccgac aatattgata atctgaatag atccgcagag tatataaaag aggtctttat
      241 cagtagcggt gccagagtta ccgcgcagga tatccccatt acgggtggcc cctacaaaaa
      301 cattgttgct aattatggtc ctgccgatgg accgctgatt attattggtg cgcactatga
      361 ctctgtcagt agttacgaaa acgatcaatt gacctatacg ccgggcgcgg atgataacgc
      421 cagcggtgtg gcaggattac tcgaactggc acgtttgtta cagcagcaag caccgaaaac
      481 aggcgtgcag ctggtcgcct atgcgtcgga agaaccgccc ttctttcgta gcgatgaaat
      541 ggggagcgcg gtacatgcag cttcgcttga gcgtccagtg aaattaatga tagcgctgga
      601 gatgattggt tattacgact ctacgcctgg tagccaggat tacccatccc cggccatgtc
      661 ctggctttat cccgatcggg gagactttat tgccgtggtc ggcagaatgc aggatatcaa
      721 cgccgttcgt caggtaaaag cggcattgtt gtcatcccgg gatttatctg tttattctat
      781 gaatgcccca ggttttattc ccggtattga tttctctgat caccttaatt attggcaata
      841 cgatattccc gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt
      901 tcccggtgat atcgcagaca gattaaatta tcagaaaatg gctcaggtag tggatggagt
      961 tacaaccttg ttatacaaca gtaaataata aaaatttctc gaggcggatc cagaaaaagc
     1021 aacatgcgca ttttattttc tttcgtctgt tttaagatgc gaaacatgaa attcaacgga
     1081 ggaaaaataa atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca
     1141 actgcttgag tctactaaaa aacctttgat ggataatata catgacacgc tttcaggatt
     1201 acgccgactg gatattgata aacgctggga ttttttacac tttggcctga cgggtacatc
     1261 tgcatttgac cccgcgaaga atgacccgct aagtcgcgca gtgcttggtg aacacagcct
     1321 agaagatggc atagatggct tcctgggatt gacctggaat caggagttag ccgcgacaat
     1381 cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact
     1441 caatgaaatg gagatttatc cgggagtaac cttttcagaa gagttagagg ggcaactctt
     1501 tgcttcgatc atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
     1561 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcaccc aaaaaaaccg
     1621 ccgaacatgt cggcggcttt ctgacttaca aactgactgc ttacatcagc ggcattgcta
     1681 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
     1741 agatcagcac cacgataccg cctgcgctgt actgccagtt tgatggtgca tcgcgacccg
     1801 gttgttccgg agcgtgaagg tataagctca ccgccacacg caggtagtag tagaggccga
     1861 ttgccgaacc gacaactacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg
     1921 ccagcacgta gaacttaccg ataaagccca gcgtcattgg gataccagcc agtgaaagca
     1981 tcatcaccgt catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga
     2041 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     2101 cgccgaggct gctgaacaga taacctgcca ggtaaacccc taccgcttcc atcgacatct
     2161 cgccggtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     2221 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     2281 aggcaaaggc gataatcgcc agcactacgc gaatcgcttc actgtcaccc accggtgcgt
     2341 aaaggaacaa acgcatcacc acgccgaaga tagcgatttt gctcgccgtc gccaggaaag
     2401 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     2461 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     2521 cgttgagcat accgtcgccg aggtttttac ccagtgcgac aaacgacagg tcgccagact
     2581 gcgcatacac cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg
     2641 tgtatttgat actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa
     2701 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     2761 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt
     2821 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     2881 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagtggcgta acgtccatag
     2941 cgcccgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     3001 gcgtagcgtt gagaaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     3061 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     3121 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     3181 cggagtgcga ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca
     3241 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     3301 tctggctttt cgctttaccg aaataagcgc gatgcagcat cgccagcgaa taaacagatg
     3361 caaagaccag cccaaaggta gagataacgg taattaccgg gacaacctgg aagctaccga
     3421 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     3481 caaagaacag cgacagtgct ggcagccact tcatcttgct ccacagaccg cccatcatgc
     3541 gcatgtcgcg ggtatggata cgttcataga gctgaccgca gagaataaac agacccgccg
     3601 ctgacaagcc gtgtgcaatc atctggatta ccgcgccctg gtaggccaac tggctaccgg
     3661 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     3721 tgatatcggt ctgggcgaag gccatccacg caccatagaa gatgccgata acacccagcc
     3781 acatagcgat tggcgcgaac tctgccgacg cgttcgggaa cagcggtagg gagaaacgca
     3841 gcagaccgta agcggcggtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     3901 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttcactg
     3961 cgaaggcgat gaagaagccc agcatcaaca ggtattccac gccgttagac atcggcgtat
     4021 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acgaaaacca
     4081 gcgcctggat ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     4141 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     4201 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     4261 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctggt
     4321 atttttcgat ctctttccac gaacagagta ccgccagcac accgagcaga ccggtcagca
     4381 cgaccatcag caacgacaga ccgtcaatgg cgagatggat agagatacca aaacgcggga
     4441 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     4501 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gttaccagcg
     4561 cgatccagcg cggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccga
     4621 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     4681 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     4741 agtgtcggat gcgccgcgaa gacggcttat ccgacctacg ttcttatatg cccaggggca
     4801 atcccacaat ccttaactca acgcagtacc atcaacagag ccagcaccac gaccgcaccg
     4861 atgctcatgg atgccacata ccagcgcaga taaccgttct cgcttaacag cagaccttta
     4921 cctgcaaagc gggaaaggac agccgggatg ttcatcattg agttcagcgg atcgcgtttc
     4981 agcaaccagg caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag
     5041 ccccaggcgt tgtaccacca ggtacccagc agacggcccg gcgcactgtt ggcgatggag
     5101 gtcaccagag tacgtttacc cagccacagc caggcagcca gcagaatgcc gaccaccgcg
     5161 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagctc cgtcgtttgc
     5221 ggaagcacgc cctgcagcgg cggtacaatc agtgcaccaa cgaaggtgga aaggatcagc
     5281 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     5341 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     5401 accagacctg ccaccatcag attgatatgt ccattcgcca tcgcacccgc gaggatctca
     5461 tccttactga agaagcccgc agtgaccagc ggcagtgccg atagtgctgc gccgcccacc
     5521 aggaagcaga gataaaccag cggaatagat ttacgcagac cacccatctt aaagatgttc
     5581 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     5641 aacgcgtggg tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     5701 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     5761 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc
     5821 agaacttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     5881 gttaccattg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     5941 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     6001 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     6061 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagcgc aaaagcgagg
     6121 aagacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     6181 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     6241 tacatcaaca gtaggttatc ggcgagaacc agaactacca tgctggcgat gaacaggttg
     6301 gtgtaagcga agaagcgaga gtagccctct tctccgcgca tataccagga ggcatacatg
     6361 tgaataagga aacccacacc agtgaccact gagagcatgg tcagcgacag gccgtccagc
     6421 accagattga aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggcta
     6481 tatgcctgct caccgttagc gaagaagtca acgccgataa aggcggttac cagcgccgcc
     6541 aggcccacag agccaacgcc gacgatcgcc gagacgtttt cagaccagcg cccacgggag
     6601 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     6661 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     6721 gcagcgcaag gccgatactc gcttccgccg ccgcgaggct gatggcgaga atgtacatca
     6781 cctgaccgtc ggtctggccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt
     6841 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     6901 ttaagccaag aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     6961 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     7021 acgctcttca cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc
     7081 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     7141 tggcgtaccg tcgatacctt gatcgttaac accgagaatg gcgtaaacaa tcaccaccag
     7201 catgatggct gacaaaattg ccggaccaat ccacacctgc ggtttcaacc actggcgttc
     7261 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     7321 aatggcaccc gcatagacga taatttccag cgcaccggca aagtaagcgc ccagtgagaa
     7381 gaacacccca gagatcgcca gcagcgaaat aatcaggtac agcagcgcgt gtaccggatt
     7441 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     7501 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     7561 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     7621 tattccgggt atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc
     7681 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     7741 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     7801 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     7861 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccggatcgcg ggtcagaacg
     7921 atacgaccac gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg
     7981 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     8041 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     8101 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     8161 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     8221 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     8281 caccgaagaa cagcgtcacc atcagtgcag agatggtcac aatcccgatg tattcgccca
     8341 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     8401 gctcggcttc cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa
     8461 aggtaataaa accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga
     8521 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcaaggaa agcccgagga
     8581 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcagc gagtatttgt
     8641 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     8701 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaattg
     8761 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     8821 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     8881 ggagcgaacc gccccagcca acacggttag gtccataacg gttctggaac agacccagca
     8941 ggcgacgttc gccaaagctc atgaatgccc cacaggtgac taccaccagc aggatcacca
     9001 ccgctttaag gacggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     9061 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc
     9121 catcggtaag cccacctgcc ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac
     9181 cgtgttgcca tcgtaactaa aggagacgcg tgtacctgcg ttcaccccca acttcgcggc
     9241 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     9301 ctgtgacaat tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc
     9361 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccgtttt cgctggtttc
     9421 aaacagacgc acgcccggat cgccaaaacg cagtttgccg cccacttcgt cctggaattt
     9481 gttccacgcc tgcggggagt tccagcctgg tgcccaggca aacggcactt gcgaacggtg
     9541 cgcggtcggc tggttgttac cttccatcga gaaggtgaac atagtgtcaa tatcctgcgg
     9601 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tatagcgatg
     9661 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     9721 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacgtggt cgagctgcgt
     9781 ccagtccact tcacggctca gcagggtgct atgcagcgag tgcagccagc gccagctttc
     9841 cagcatgaca gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc
     9901 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aagctggcgg cggaaagtac
     9961 cagatgggcg ttttccataa tcgctgtgcg ttgatggtca accaccatca ccagcggcgc
    10021 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
    10081 caccaccacc gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgatcc
    10141 accgcccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccgac
    10201 gtcagcgccg cgacctttca gggctttcgc cacgttagcc gcagcctgaa tcacttcagc
    10261 gctaccagcg ttcgtcccgg agataatcaa cggtttcttc gcaccagcca gtgcctgcac
    10321 gatgacgtcg attttgcttt gcagctcagg ctcaataccg tcaaccgctg gtgcagagtt
    10381 atccagcgca tgagcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
    10441 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
    10501 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
    10561 tgccgccatt tcgcgcgctt tacctttcac cgcctgacgc actgccagcg cgacgcgcgc
    10621 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
    10681 cagagccgga gtataaatgc cgccttcacg cagtactttc agcgccagtt gcagacgttc
    10741 ctgctcaccg tgagcgatac cggtatagaa gttttcttcg cccaccagct cacgcagcgc
    10801 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
    10861 cagaatatct gctgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
    10921 acgacgctgt actggctgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg
    10981 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
    11041 cagttcgcca taacgctcgc cggggctgat gttacagccg atggaacatt gctggcagat
    11101 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    11161 gaatacgccg gtcggacaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc
    11221 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca ggtccgtacc
    11281 gtcagcgtaa tctttgtagt aacgcacgca gcggtagcag gcgatgcagc ggttcatttc
    11341 gtgagagatg aacggcccca aatcctgatt acggtgagta cgtttggtga aacggtagcg
    11401 acggaagctg tgtccggtca tcacggtcat atcctgaagg tggcagttac cgccctcttc
    11461 acataccgga cagtcgtgcg ggtggttggt catcaaccac tcgaccacgc tttcacggaa
    11521 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    11581 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    11641 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    11701 cagagacaga caagcttcca gcaggttgtc cgccccgttg acctcgtatt ctttgccgtc
    11761 tacatgaatt gtagccatta gcatgcttcc agttttctca gtcagagact gagcgttaat
    11821 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    11881 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    11941 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc ggacctaaga
    12001 atcgacacag ttgctcaagt gtttcgatat cgcccggctg accttcacca cgctccagcg
    12061 cacgcagaat tttcacgctc cacggcagac cgtcgcggca tggcgtacac cagccgcagg
    12121 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgtta atctcgtggt
    12181 caaccgccat cgccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt
    12241 ccatcggcag gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgccg cctggctgcc
    12301 aggctttgaa tttcaggccg tcacgcatac caccagcgta atcttcaagg atttcacgcg
    12361 cggtggtgcc gaacggcagt tcccacagac ccggattttt cacccgaccg gagaagccca
    12421 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    12481 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt aacgcaggtt ggtttacccc
    12541 atgcaccgga ggttgccggg aagggcggct tcgagcgtgg gttagcgcga cgcccttcca
    12601 gggagttgat taacgctgtt tcttcaccgc agatgtagcg ccctgccccg gtatggacga
    12661 acagttcgaa atcgaaacct gttcccataa tgtttttgcc aagcagaccc gcttcggtgg
    12721 cttcggcaat ggcacggcgc agattcactg ccgcttcaat atattcgcca cgcaagaaga
    12781 tgtagccacg atacgctttc agcgcaaacg cagaaatgag cataccttcc accagcaggt
    12841 gcggtagttg ctccatcagc agacggtctt tataggtgcc cggctccatt tcatcggcat
    12901 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    12961 cagtagaaaa gcccgcgccg ccgcgccctt tcagaccaga gtcttttacc tgattaacga
    13021 tttcgtccgg agacagcccg gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc
    13081 gatattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    13141 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    13201 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    13261 ccccaggcag caagttggca gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt
    13321 gatgttcagt tttttctcca gcgccgcctg aataccctgg taaccgttga tatgacagac
    13381 cacgctgtcg caataacgga tcacatggcg gcctactggc tgacggaaga tctggctata
    13441 aaacgttgcc acgccttcga cgtcgctcgc cggaataccc agcacatcgg cgatcgcgtg
    13501 gatcgcgcca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    13561 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    13621 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    13681 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    13741 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtacggat acgggtgcga
    13801 taactcatgg tgctgccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    13861 accatctgga aggattcatt ggcaggcatc accggacccc acgacacttg caggaagtgg
    13921 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcgga
    13981 tgatccgcct tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    14041 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca
    14101 cccaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    14161 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    14221 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtgtt ttgcagcgcc
    14281 gctttctcgt aagacgccag acgtttaggc atccagtcga ggaactcacg cagcaggcga
    14341 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    14401 cgaaaaccag tgattgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    14461 aacactggcg tcatcgcgcc gacgtcctga ataaaggtgg agatatacag caggtgactg
    14521 ttgatgcgga acagttcgga gagcattacg cgaatgacgt taacgcgatc cggcacggtg
    14581 atcccagcca gtttctctac cgccagcaca taaggcattt cgttaacgca gccgccgagg
    14641 tattcgatac ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc
    14701 tcagcgccac ggtggtggta accgatgtct ggtacgcagt cgacaatctc ttcgccatca
    14761 agctgcaaaa cgatacggaa agcaccgtgc gcagacgggt ggttcggacc gaggttgagg
    14821 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    14881 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    14941 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    15001 cggcgcaggt tcgggtgacc gtcgaatgta atgccaaata gatcccaggt ttcacgctca
    15061 taccagttag cgttcgggaa tagtttggta aaggtcggta cgtgcaggtc gttttctgcc
    15121 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    15181 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    15241 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc cacttccagt
    15301 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    15361 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcgcgg
    15421 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    15481 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    15541 ttccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    15601 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    15661 tcgccaacca cccaggagag cggacgacgt tctttaccga tagattcctg caacagcatc
    15721 agtgcctgca tgtacgcttc aggacgcggc ggacagcccg ggatatacac atcaaccggg
    15781 atgaatttat caacgccctg cacaacggaa taaatatcgt acataccgcc ggagttggca
    15841 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacaa acgctgaata
    15901 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    15961 gaagcacgca atacttcagc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    16021 accatctcaa cgtaacagca ggaaaggccg aagttatacg gccaaattga gtttttacga
    16081 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    16141 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    16201 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    16261 atactgtccg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    16321 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    16381 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    16441 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    16501 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gaacgttttt tgacctcgcg
    16561 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    16621 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    16681 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    16741 cacatcgatt catggggagg aataaaaaaa accttacaat aactgcagaa actattttat
    16801 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaagg
    16861 tttcttcaca ctgatttaca caatgacaac aacatttgca tttggcgccc tttctcccca
    16921 gatcattgac gactccagga aaattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    16981 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    17041 attttcttga tctgtgacac gcttttgtca ttctataaca aaaacgcagc aacaaattca
    17101 cgtatttttt aacattattg tagcaggaga tttttttcag gtgattattt gtgcgtacgg
    17161 ggcgtgaatc tctggtgatt gaaaaatgaa cagttttata cgttctgcac gatacgagga
    17221 aggcttttta ctaaaaaaaa gccgctgggg tttaaaataa acccagcggt tcgtttttac
    17281 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    17341 atgagtcatc tccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    17401 ccatcgtctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    17461 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg
    17521 gactcatcat ctcgaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    17581 cggccggaag cgtcgaggcg acataagcca gtcgccaggg aatatctgct ttattaagcg
    17641 tcgccagcac catatcgcga aacggacttg gatcatccag caacacaaga gggatcggct
    17701 cacctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt
    17761 tcaatgcctt aaaggtagaa ggacgatggg tggttaccat caaatccact tcctgggatt
    17821 ccagcatttc tgccatgtag gcattacgtt taacgcggac atccagcgct agtttcggat
    17881 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    17941 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    18001 taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    18061 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    18121 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    18181 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    18241 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    18301 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    18361 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    18421 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    18481 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    18541 tctcgtacag catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    18601 caagagcagg gatccgtcat ttatgcaagc attttacccc aaaaaccttt tatttataag
    18661 ggtcattgcg aattatctga tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta
    18721 ttaataagca caacaaaacc tttttgaata ttaaataata attaattagc ttcatcccca
    18781 ttcattaatt ccgcttaaca atagtttcac accttgcccc cattgtgaca cctcggaaat
    18841 catcacgctg ttaaactctc cgtaatgcgg aaatatcatc atcgcgattc agtagtgaat
    18901 tcttaaacga ggcattttca cacaattatc ttacagataa aaaaaccaca cttacaatta
    18961 agaatcagaa caattcacta cataacatta cttattaagc atatgcacct cattattttg
    19021 ccattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat cttattctgc
    19081 agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg
    19141 agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat
    19201 tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag
    19261 aagaaggtaa caaggttctg aaactgaaca tcggcaaccc tgccccgttc ggttttgacg
    19321 cgccagatga aattctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg
    19381 attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca
    19441 tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc
    19501 aggcaatgca agcattgctg aacagcgggg acgaaatgtt ggttcctgca cctgattacc
    19561 cactatggac cgcggcggtt tcactctcca gcggtaaagc ggtgcattat ctttgcgatg
    19621 aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc
    19681 gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt
    19741 taatggagat tgtggagatt gcacgtcagc ataatctcat tatctttgcc gatgaaattt
    19801 atgacaaaat tctctacgac gacgctgagc atcactccat tgcgccgctt gcacctgacc
    19861 tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg
    19921 ggtggatggt gttgaacggg ccgaaaaaac acgctaaagg ttacatcgaa ggtctggaaa
    19981 tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcac
    20041 tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc
    20101 gtaaccgcgc gtgggaattg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg
    20161 gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac gacgatcaga
    20221 aaatggtgct ggatttcctg ttgcaggaaa aagtcctgtt ggtgcaaggg acggcattca
    20281 actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc
    20341 tgtctttgag taagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc
    20401 actgccggag attgcatccg gcagtgttat cccgccacaa tgacctgatg atgtcatcat
    20461 acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc tgaaactcat
    20521 taaccgctgg ccgctaatgc gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca
    20581 ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cgtaaatttg gcggtaatgt
    20641 caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcttcagaag tgctcaccgg
    20701 agatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggaat acaaggctat
    20761 tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctac aggatatctt
    20821 tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc
    20881 agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt
    20941 cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga
    21001 ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga
    21061 ttcaccgctg taagcagctg gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca
    21121 tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcacg
    21181 ataatggtga acggtacccc caacttaaca aagtcactga agctgtaatt ccccggacct
    21241 aaaaccagtg tgttaacagg tgaagatatc ggtgtcataa aggcggcgga tgctgccatc
    21301 gccacgacca tcgcaaatgg ataaggcgac acccccatcg ttttggcagc agccagcgca
    21361 atcggagcca tcaacaccgc cgtcgcagta ttagaaataa atagcccaat aaccgccgac
    21421 aagacaaaca aacaacccag catcatatgt ggcccataac cgccgccaat gtccatcagc
    21481 cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc
    21541 ccaacgatca aaataatgct cggccagtga atggatttat aggcactttc ggcatcgata
    21601 cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca
    21661 tcggtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg
    21721 tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgccacgaa gtcgcggccc
    21781 tgtttggcca gcataccgat cagtttccag ttaccaacaa ccagaatgat atcgcccagc
    21841 agcagaggct catccgccag cgaaccttcc agcgccacac cattgcgctt tagccccacc
    21901 acattcaatc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aataagttct
    21961 gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac
    22021 tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca
    22081 tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta
    22141 acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcacca
    22201 taacgttcac gtaatttgag atcgtccagt cgttgaccaa tcattggcga tccggggcga
    22261 atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt
    22321 cggcgcgtcc agccttcgcg ttgtggggtc tgggtatccc ctttcagcat gaaacgcatc
    22381 actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag
    22441 aaactgaagc cgtgatagcc ttcacgcagt aattcactgt tgactaccag gttcggcggc
    22501 gtcgccacca gcgtcatcat gccgcttatc agcccggcaa aacttaacgg catcatcaga
    22561 cgcgacggcg acgtttgcat acgcatggca acgcttaaca ccacggggat aaagatagcg
    22621 acaacgccgg ttgaactcat aaacgcgcca agtcccgcga cggtcagcat caacaaaacc
    22681 aacattttga tttcactgtt gcccgccact ttgaccagcc atgtccccat tacagtggca
    22741 acaccggtac ggaccaaacc atcgccaata atgaacaagg cggcaatcag gacaacgtta
    22801 ggatcggaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg
    22861 acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacgatg
    22921 gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa
    22981 ttgaggggga taacttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg
    23041 atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct
    23101 ccagactgtg gaggactaaa tcgacttcat tcaggcgcgg ggtatctgcc ggggcattaa
    23161 ccgcaataac atggcaaccc gccgccagac ctgaaagcac gccagcggga gcatcttcca
    23221 ccacaacaca ctcctgcggc gcaagcccca gcagctgcgc gcctaacaga tacgcatcag
    23281 gttctggttt tccgcgcttc acccgctcag cggttacaaa cacctctggc gcgggaagcc
    23341 ccgctatttt atgacgcgct cgcgctaccg gcatggagcc agaagtcaca atagcccacg
    23401 gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc gcggtaatac
    23461 cttcggtttc cgtggcctcg atgtgttcca gacgcgtaaa ctcggcggca atgtctgcct
    23521 cggatttgcc cgccataaaa tggcgcagag aggtgatcgc ctgtttaccg tgaatgaaag
    23581 ccagcacctc ttccggcgat aacccatgac gtctggccca gttgctccac gcccgttcta
    23641 ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca
    23701 cgcgggcctc ctcaggcgtt aataatttga ttaatttcgt tggcgctcaa atggtactga
    23761 cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg
    23821 gcgttaaaac cgtgagttcc agcgtcaaaa tgggtatagc gcccttccac attaaccata
    23881 aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca
    23941 cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga
    24001 tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg
    24061 cgatccagtt cacgcatctg taatccataa ccacgctcaa tgattgtttg caggcgacgg
    24121 taacgttcag catttgccgg atcgagcata gtcatcatct tgtactggtt agacaaaata
    24181 agacgttgcg cgttggtcat ttccattatt gactcctgta tcactctact acggtgaaaa
    24241 aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc
    24301 aaggaaagtt ttatccagtt gtttgaaggc acgcttaagc gtgtcagcta atgcctggta
    24361 atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac
    24421 ttcataaaac cagttgagga ttgcaggagg taatggcgtt acagaacgct tgcccagcca
    24481 ccacaatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag
    24541 ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg
    24601 gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagactgggg caaggcgttt
    24661 ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac
    24721 agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat
    24781 ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact
    24841 atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat
    24901 tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc
    24961 ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca
    25021 tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc
    25081 catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat
    25141 catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc
    25201 cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg
    25261 cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga
    25321 actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac
    25381 cagctccgta gtgattgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc
    25441 accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca
    25501 gctgaaagac aaaaacgttg ctgtatttga caccgcattc caccagacta tgccggaaga
    25561 gtcttatctc tacgccctgc cgtacaacct gtacaaagag cacggcatcc gtcgttacgg
    25621 cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc
    25681 ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat
    25741 ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat
    25801 gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg
    25861 catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac
    25921 cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa
    25981 gcgcgcaatg gacgtttact gccaccgcct ggccaaatac atcggtgcct acactgcgct
    26041 gatggatggt cgtctggacg ctgttgtatt caccggtggt atcggtgaaa atgccgcgat
    26101 ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg
    26161 caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc
    26221 ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc
    26281 ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt
    26341 aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc
    26401 ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg
    26461 agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg
    26521 actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac
    26581 gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat catcgcgaac
    26641 taccacgcta acaccaaaga cgctgaagtc gttctggtgg aaggtctggt cccgacacgt
    26701 aagcaccagt ttgcccagtc tctgaactac gaaatcgcca aaacgctgaa cgcagaaatc
    26761 gtcttcgtta tgtctcaggg cactgatact ccggaacagt tgaaagagcg tatcgaactg
    26821 actcgcaaca gcttcggcgg tgcaaaaaac accaatatta ccggcgttat cgttaacaaa
    26881 ctgaacgctc cggttgatga gcagggtcgt acccgtccgg atctgtccga gatttttgac
    26941 gactccacca aagcaaaagt gaacaacgtt gatccggcga agctgcaaga atccagcccg
    27001 ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat
    27061 atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt
    27121 aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt
    27181 tctctgctgg tgacttccgc agaccgccct gacgtgctgg ttgccgcttg cctggctgcc
    27241 atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc
    27301 atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc
    27361 aacacctggc agacttctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat
    27421 cacgagcgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc
    27481 gattctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgccagc gttccgttat
    27541 cagctgactg aacttgcgcg caaagcgggc aaacgtatcg ttctgccgga aggtgacgaa
    27601 ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg
    27661 ctgggtaatc cggcagagat caaccgtgtt gcagcctctc agggtgtaga actgggtgca
    27721 ggcattgaaa tcgttgatcc agaagtggtt cgcgaaaact atgttggtcg tctggtcgaa
    27781 ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg
    27841 gttctcggta cgctgatgct ggaacaagat gaagttgatg gtctggtttc cggtgctgtt
    27901 cacaccaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc
    27961 tccctggtat cttccgtgtt cttcatgctg ttgccggaac aggtttacgt ttacggtgac
    28021 tgtgcgatca acccggatcc gaccgcagaa cagctggcag aaatcgcgat tcagtccgct
    28081 gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt
    28141 acttctggtg ctggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa
    28201 aaacgtcctg atctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac
    28261 gttgcgaaat ccaaagcacc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc
    28321 ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc tgacctgatc
    28381 tctatcggac cgatgctgca gggcatgcgc aagccggtta acgacctgtc ccgtggcgca
    28441 ctggttgatg atatcgtcta caccatcgcg ctgactgcaa ttcagtctgc acagcagcag
    28501 taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa tagtcactat
    28561 ttccggtttt ttattctctt aatttgcatt aatcctttct gattatcttg cttaactgcg
    28621 ctgcatcaat gaattgcgcc atctcacttt gcatacttac cactttgttt tgtgcaaggg
    28681 aatatttgcg ctatgtccgc aatcactgaa tctaaaccaa caagaagatg ggcaatgccc
    28741 gatacgttgg tgattatctt ttttgttgcc attttaacca gccttgccac ctgggtagtt
    28801 ccggtgggca tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca
    28861 cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag
    28921 tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggtctgat gaacttcccg
    28981 tttgaagggt taacctcagg atcgaaatac gggagagccg ttggcatcat catgtttatg
    29041 ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc
    29101 ctggcgctta ttcgccatac ccgcggaaat gaaattctct ttattcctgc gctgtttatt
    29161 ctgttttcac ttggcggcgc aatatttggt atgggagaag aggccgtcgc ctttgccatt
    29221 atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt ccttgtgacc
    29281 tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggttgtt
    29341 gctcagggta ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg
    29401 gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag
    29461 aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat
    29521 gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta
    29581 atggtctggg ttatttgggg cgtgatcgtt aatgcctggt ttattcctga aattgccagc
    29641 cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc
    29701 atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct
    29761 gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt
    29821 gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag cggtctggat
    29881 aacgcagtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg
    29941 tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc
    30001 ggtgttaacc gtcaggttac cgtgctggcg ttccagtttg gtgatggctt cagccacatc
    30061 atttacccaa cctcagcttc gttaatggcg acgctcggtg tttgccgggt ggacttccgt
    30121 aactggctga aggtgggtgc gaccctgctt ggactgctgt ttattatgtc cagcgtcgtg
    30181 gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgcg attgcggctc
    30241 ttttttcatt ctgccgtttc agtctcgacg gcttcatttt tggcattgcg cttcatccac
    30301 aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc
    30361 ggcgtcagcc agcaaacttc actgacttca tcttcctgta gagcgaaggg accgtgagag
    30421 acgcagctga acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg
    30481 tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat
    30541 tccagcagct gctcatcggc ctggactaca ccgcctgcgg tcgcatctaa catgccgggt
    30601 aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg
    30661 acgatataag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt
    30721 gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc
    30781 tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg
    30841 gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca
    30901 ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt cggaatactc
    30961 accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta
    31021 tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga
    31081 tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt
    31141 atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata
    31201 accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt
    31261 aaagcattac gcgggccatg attcaacacg tcgccaagga tcaccagcca ctgggcaccg
    31321 ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat
    31381 gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt
    31441 ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc
    31501 tcgctgccac tggaatgtcg cataatccac caggcgttcc ggcacctcat cgccatgtag
    31561 taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa
    31621 attcggctga cccagtgcta acaaatgctc tgccatcgcg aacagtttct ctgcactggc
    31681 ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcgggcg
    31741 ttcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc
    31801 acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc
    31861 cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg
    31921 cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag
    31981 gtcgatggtc ttgatatgaa acgacaggcc tttctcctgc aacgccaccc aggcggataa
    32041 cacataaggg gagaaaaaat gggcatctga ccaaagcgta atagcgggtt tactcattat
    32101 gtcctcggca ataaaggggg cttgctatca aactatagag cctttttgtc actgtcacgt
    32161 gacgaatact cacgcaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattagaca
    32221 accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg
    32281 ctgtttctcg aagaagcagg gctggattat cgcttgataa aggtagacct ggggaaagga
    32341 ggtcagtttc gcccggaatt tttgcgcatt tcgcctaaca acaaaattcc ggcaattgtt
    32401 gatcattctc cagccgatgg cggcgaaccg ctaagcctct ttgagtctgg tgccattttg
    32461 ttgtatctgg ctgagaaaac gggactcttt ttgaatcatg aaacgcgtga acgcgccgtc
    32521 acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat
    32581 cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa
    32641 actcagcgtc tgtaccatgt actgaacaag cggctggaaa attcgccctg gctgggaggc
    32701 gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag
    32761 cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc
    32821 cctgccaccg ggcaggcact gctaaaagca caacacggtg atgagcgttc ggatagttaa
    32881 cagaaacagg ttctcgtgta ttatttcatt ctaagtaaaa caacggagaa cctgcaatgg
    32941 cacaacctgc cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta
    33001 aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg
    33061 ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga
    33121 aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg
    33181 atgtgctcga tatcgcacgc gaacatcact gggtgacgta tgctgaagtg gagatcgata
    33241 aactgcacgc gctgcgctac accgattcgg tatccatgac cttaagctgg cagcgttaac
    33301 caccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg
    33361 ccatttgatt ccacgtttgc tggacctggg ccatcaaatc acggtagtga cgcgtaaccc
    33421 gcagaaagcc agttccgttc tcggcccccg agtgacacta tggcaagggc ttgccgaaca
    33481 aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa
    33541 acgctggact cacgagcgaa aagagcgtct ctgccaaagc cgctggaata ccacgcaaaa
    33601 actggtcgat ttgattaatg ccagcgacac gccaccgtcg gtactcattt ccggctcggc
    33661 aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa
    33721 cgaatttacc cataaactct gcgcccgctg ggaagagatt gcctgtcggg cgcaaagtga
    33781 caaaacgcga gtgtgcctgc tgcgtaccgg cgtagtgctg gcaccagacg gtggtatcct
    33841 cggtaaaatg ctgccgccat ttcgttttgg cctgggcggg ccgattggct ccggtcggca
    33901 gtatctggcc tggattcata ttgatgatat ggtcaacggc attctctggc tgctggataa
    33961 cgagctgcgc gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc
    34021 ccatgcgctc ggtcatgcgc tgcatcgccc gaccattttg cgcgtccccg cgaccgccat
    34081 tcgactgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgccaaa
    34141 aaagctggaa gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga
    34201 tgtcgttcgc tgatgtggtt tactgcaaac atccgccagc ggaaacgctg atgaataaat
    34261 atagataatt cagaactatt tttagcggac tgcaactttt acatttcagc ggaagcggac
    34321 tcgcaaattc cccatgaaat actccggcag cgttaaccac cggagaggaa attatttcag
    34381 cgaaccttta aggaactgtt gcagacgagg gctttgcggg ttaccgaata actgctccgg
    34441 cgccccttct tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatgacg
    34501 agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg
    34561 cataatacgc aacacttcgc ctaccagttc aggatcgagc gctgaagtag gttcatcaaa
    34621 cagtaaaact tccggttcca tcgctagcgc ccgcgcgata gaaacacgct gttgctgacc
    34681 accggacagg tgcaccgggt atttcccctg cgcacgttcg tctatcccga cttttgccag
    34741 atacttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc
    34801 ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct gaaataccat
    34861 cgtcaggcgt gtgcgcaata agcgcagctg atttttatcg gcgactttga gttgaccatc
    34921 tttatcgcgc accagattga tcgtctggcc attgaccacg atcgatccat cgctcggttt
    34981 ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat
    35041 gcttatgaca tctccggcat tcgcttgcaa tgagacccct ttcagcactt cgtgttcgcc
    35101 gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg
    35161 ttgaagaagg tttcacatgc tgcaaccagc gtttttcagc tctacgaaag aggctgatca
    35221 gaacataaga gatgattaaa tagagcaccg cggcaatgcc gaaagcggta aaaggttgat
    35281 atgtggcggc gttaatatcg cgggcaattt tcagcagatc cggcaccgtg gcagtaaatg
    35341 ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac
    35401 gcagcgcaga aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc
    35461 tggcggcttc aatttcccca tgcggcactg aacggattgc cccggcaaaa atctcggtag
    35521 tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcgaaaaa
    35581 aggcgttaag gaactcggtt cccttaacaa tctccagcgt gtacatgccg gaatagaaca
    35641 ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa
    35701 actggatgta tttattactt gagacgcgac caatcgccag aaacagcgcc agaactccgc
    35761 ctatcactac cgacaaaatc agcagccaca gggtgatcgc cacgccagta aagcgataac
    35821 cgtcagtcca cagcagtggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct
    35881 cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt
    35941 gaaaacaaga taaatcacgc cgcagacgat agcgaaatag aacggttccc aggtactttt
    36001 gcctgccagc tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt
    36061 agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc
    36121 cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg tcgccgcctc
    36181 tatatgtcct ttcggcactg ccataaacgc gccacgaaac gtttcggtaa aataagcacc
    36241 gtagataaaa ccgagagtga taatacctgc gaccatcgga tcaatatcaa tctgcccgac
    36301 gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa tcagcagcat
    36361 caatactaaa tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa taaggcccga
    36421 aagccgattt tgtgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac
    36481 tacagagctg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacttgaaaa
    36541 cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc
    36601 cggttacccg aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca
    36661 aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag
    36721 gctttgttca gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct
    36781 acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc tttaccgacg
    36841 ggttgtttga ggaaaccttc gctggctgcg acctcatcct ggaacgcggc atcaatacgt
    36901 ccggcagtca ggtcagaata aatgttgtcc tgcccctgat aagaaacgat ttcaatgcct
    36961 tttggtgccc agtgttcgtt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc
    37021 cgtttgcctt tcagcgattc gactgtcggc tgaatgtcgg aatttttcgc caccaccaga
    37081 cgagaatcgg ctgcgtacag tttgtcggtg aaggctattt cttgctgacg tttttccgta
    37141 atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca
    37201 tccagtgggt tttcgacaaa cgtacattgc gtattgatgc gtttgcacaa ctccttggcc
    37261 agatcgatat cgaagccaac cagttcacct tgtgaatttt ttgattcaaa tggcgcatag
    37321 gtcggatcgg taccgatgcg gatgttttgc ggaatcgcag caaatgctgc agttgcgctg
    37381 gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac
    37441 agtcttttta tcgggaatat ttacaggttt gttacactta tcgtgccata aaattgacca
    37501 tcaaggcaaa atatcccgcc ccgctagacg tattattttt cattaaaaat gcttaagtat
    37561 gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat
    37621 caggtacagc ttccctgcga cgtatcagtc accgtagaca ttaaagtcaa aatacttttt
    37681 cgccatcttg tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat taaacgcagc
    37741 cgtcagttcc gcatcatctt tacgtagccc tacaccggtg ccgtcaccga agtatttttt
    37801 gtctttcact gatgggcctg caaaggcgaa atctttaccg gcgggttgct taaggaaacc
    37861 ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga
    37921 atagactaaa tcctggttgg cataggccac cacatccacg cctttgctac gccaggtctc
    37981 gttagcgtaa gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga
    38041 atccagcgtt ggctgaatcg gtgaaccttt ggcggcaatc aaacgagaat ctgcggcgta
    38101 cagcttgtcg gagaaagcaa tctcctgctg acgtttatcg gtaatggaga gcgacgaaat
    38161 aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cgctggcaac
    38221 ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc
    38281 aacaaagtca cctttggcat ctttcgatga gaacggtgcg taggtggtat cggttccgat
    38341 acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaacaa
    38401 agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg
    38461 ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga
    38521 cagcagtaac attcaacgtt aaatatgtta ataagaagtt gcattattgt cctaaagttg
    38581 aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt
    38641 cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc
    38701 cctgccagcg ggcaaagaga tcttcaggaa gggttaccgc aaactggtca agaacacgat
    38761 taaccgtctg atttatcaca tcatcaaggg attgtgggcg atgataaaac gccggaacgg
    38821 gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt
    38881 gcagtggcgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg
    38941 ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg
    39001 aacagggtaa aatcaccatc cccaacgtct ggaaagaacc ggaagaaatg ctggcagcaa
    39061 tatcgcgcgc atcgtgtgtc acatcagcta acgcctgcac ttcgcgcagg gaaaaatccg
    39121 tttcgaggga taaggtctgg cgcgccgcct ggctcattac cagatgcgtt tcgatatctg
    39181 tgacatcgcg cagaacctgt aataaacgca cgccataaat cgcgccgctg gcaccgctga
    39241 tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca
    39301 ggatttcggc gggagttgca agtcagggtg ccagaccggc accctgagcg aaggcatcat
    39361 ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtcg
    39421 tcattacgta aagtatcgag gaaatcgagg tagccctgat caacgtcttt ggtgacgtag
    39481 acgccgttga acaccgagca ttcaaactgc tggatatccg ggttttcagc gcgaacggct
    39541 tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt
    39601 tcatctactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc gataccgtaa
    39661 acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct
    39721 tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgataga gtcgtcgacc
    39781 agcaggacgt ttttatcgcg gaactcggcg cggttggcgt tcagtttacg gcgcacggac
    39841 ttacgacgca gctgctgacc cggcatgatg aaggtgcggc caacatagcg gtttttaacg
    39901 aagccctggc ggtacggttt gcccagaata cgcgcaattt ccagcgcgat atcgcaggaa
    39961 gtttccggga tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc
    40021 tcgcccagct tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgatgaac
    40081 gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac cggattgtca
    40141 gcacactgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gccaggggcg
    40201 acatcacgca ggaactcaaa gcccagcgta tcgagcgcaa cgctttcgga agcgaccata
    40261 tattctgtgc gattatcgtc aatatcacgt ttgcccagta caagcggacg aatcccgtta
    40321 ggatcgcgga aagcaaccat accgtggccg ataatcatca ccacacaggc atacgcgccg
    40381 cggattaagc ggtttgtagc agcgatggcg gcgaaaatgt tgtcggcttc cagcgggtag
    40441 tggcggaagt tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg
    40501 gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc
    40561 agattgccgt tgtgggcaag cgtaatgcca tacggagagt taacgtaaaa cggctgcgct
    40621 tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc
    40681 tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttccgc
    40741 aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc
    40801 tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg
    40861 acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg
    40921 ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaac
    40981 tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt aaaggagtcg
    41041 agaaagaaga gaatggcagc aacaatcaac acaccgcgca aggcaccgaa acagacgccc
    41101 aacacccgat cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc
    41161 acgatagcac caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga
    41221 accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca
    41281 acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgcggatc
    41341 aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata
    41401 tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa
    41461 aacgtttgcg tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc
    41521 aacgccatcg actttttatg cctttgcggc atcgggcaat gcgtgtcgga tgcggcgtga
    41581 acgccttatc cgacctacgg ctctacccct gcacaggcct gataagacgc actggcgtca
    41641 catcaggcaa cggctgtcgg atgcagcgtg aacgccttat ccgacctacg gttctacccc
    41701 tgcacaggcc tgataagacg cgctggcgtc gcatcaggca acggctgtcg gatgcggcgt
    41761 gaacgcctta tccgacctac ggttctaccc ctgcacaggc ctgataagat gcgccagcgt
    41821 cgcatcaggc aaaaccgtat taattcggtg tatagcccat taccacacca cttaagccag
    41881 aaagctgctt gagctcaccc agcgatcctt tcagcttctc tttggaagca tctggcccaa
    41941 ccagaatacg ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac
    42001 cggcaccgcg cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcgccca
    42061 gttgcacaac ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt
    42121 ctggcttcgg cgcaggtggt gcttcaacct tcggtttagg cgactccacc ggtttcggct
    42181 tcggtgggac gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg
    42241 ccggatcgag cgacggtgct gcggcatcgc ctgcccgcac ctcttccgct gcgccttccg
    42301 gcggctgcgt cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcgcgatcgc
    42361 ccgctttcgg caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt
    42421 ccagcagccc tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc
    42481 gattctgaaa cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg
    42541 accgtgtgga acgaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg
    42601 gcatcccatg cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt
    42661 tgttctgccg tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg
    42721 cttttcaacc aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc
    42781 agcacgcgcc cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt
    42841 ggattatgcg cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc
    42901 ggcaaaattg cgctggcaat cccgtcgcga atggcatttt cactgacctc cagcccgctg
    42961 gcacgcagtg ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga
    43021 ttttccagcg tgccgtgagc atcgctaaac gtccagtcat gatcggtgac ggaatagttc
    43081 cactcaacgc cccgacgttg tagcagtgca cctttttcct gccccacatc agcaatggta
    43141 gaaggcattt ccggctcacc gacaattgcc ggtttttcgc tgcgaaagat acctgctttc
    43201 tcgcgaccaa tactttcgcg atctggcccc agccagtcgg tatgatccag cgcaatactg
    43261 gttactaccg cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct
    43321 acttccagaa tcactacgtc aagttgtgcc tgcttgaaca gccacaatgc cgacagcgta
    43381 ccgtactcga aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagaa
    43441 gcggtgtggg ccgattccgg caattcctgc ccctgcacac gtacgcgctc ggtataacgc
    43501 accagatgcg gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc
    43561 agcgtacggc aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct
    43621 ggtttcagaa cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata
    43681 gttttactgt gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga
    43741 ggagtgcgtt tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc
    43801 agagccagtg gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggt
    43861 ggcaccacta cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc
    43921 gccagaatgc tcgccagttt cagacgcatt tccggacgac ggacgatcat gtcgatcgcg
    43981 cctttctcga tcaggaattc actgcgctgg aatcctggcg gcagtttttc gcgaacagtc
    44041 tgttcgataa cacgcggacc ggcaaagcca attaacgctt tcggttcagc gatgttgaga
    44101 tcgcccagca tggcgaaact tgcagaaaca ccgcccatgg tcgggtcggt cagcacggag
    44161 atgtacggca agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc
    44221 atcagcgaca tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagt
    44281 gggcagttat cttccagcgc ctgctcaacg gcacgcacga aacgcgcacc taccacagac
    44341 cccatggaac cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac
    44401 agagtgcctt tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc
    44461 agacggtctt tatacttctt ggagtcacga aacttcagca catctttcgg ctcaagctcg
    44521 ctacccagct ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc
    44581 atacgcatgt gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg
    44641 tataaaacct gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg
    44701 cgggtgggag taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc
    44761 tgaacctggt tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa
    44821 cagaccatga atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt
    44881 cgaaccgcag agttactttt tattttgcgg cgcgtgccgc tgcgcgtttg tggcgaataa
    44941 attcgaaaac acctggcaat attgaaacaa aaataatgcc aacaatcatc agtttaaggt
    45001 tgctctgaat gaaggggagc gtgccgaaga aatagcctgc gtaggtaaaa agcagtaccc
    45061 acaacagtgc gccaatcacg ttataagcgg cgaaatgacg gtacgacatg tggcccattc
    45121 ccgcaacaaa cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt
    45181 tgccgccatg tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt
    45241 tcgaattggg attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg
    45301 cgtcacccac aatcgcggcg atcagcatca gcaccaccat catatggacg ttgagatcgt
    45361 tggtctccag tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg
    45421 gcgttaccac cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga
    45481 cgccgtactc cgcgaccagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga
    45541 ggaaataaat caggtccata tttgcctatg ccttgtactc gtcatctatc aggctgtaac
    45601 tacgttggct acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc
    45661 gggcccattg gcggttttgg aagatcatat cggtcagggt aatccaccgc gaccagatat
    45721 agcccttccg cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct
    45781 gctatccagc tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc
    45841 ctgaccatat gatgtacaaa ggcattcgct ttgatatcta ccaccacata aggaccgtga
    45901 cgcgtgacat taatatgcat aacgttgcgc cacggggtcc gggactggca ctgcaccgca
    45961 cggaacgagg tgaaatcatt ctcgcccagc aaacattgcg cagcccgatg catccgttca
    46021 gcgtccagcg gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga
    46081 tgattgtaga tgatgtagcg ataacggcga gccgtggcgc taaatcgggc atgaaaatca
    46141 tcaggtacag ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc
    46201 aacgtccacg cagcgtcttt gcgctgcgcg gtcgtttcga aatgcacaac ctgcccggta
    46261 ccgtgtacac cggcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc
    46321 tgggagagcg ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag
    46381 ccgtaatacc tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcggttgt
    46441 tgctggtcgg acattagtac agatactcct gcaccagttt ctcggcaatt tttactgcca
    46501 tcagcgcgcc gccaaagcga acgttatcgg ccaccgacca gaactgaact tgctccggca
    46561 taccgtagtc attacgtacg cagccaacag aaagatgcgg cgtaccagaa gcatccccta
    46621 cctgagtcgg gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat
    46681 cacgcgcttc ttctgccgcc agcggacaca gtgcttcaaa gttgaccatc tgggcatgac
    46741 cgtagaatac cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga
    46801 ttttgcgtac ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca
    46861 gtaacggcag catgttgaac gccagctgac gcccgaagaa atcttcttcg tcaatcggaa
    46921 tgccattgag taatttagca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg
    46981 cggaggctga aatcaggctg gtaacgctga tacgtgataa accgccctga tcaattagcg
    47041 gtttcagtgc cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt
    47101 tgcggtaatc ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt
    47161 cgagagcaaa caatccgctg ctgtcgatca ccagacaacc tgagttggtc gcttcttcaa
    47221 cccaggcagc ggtagcctct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt
    47281 cgaattcagc agcatcctgc acggtgattg tcttaccacc aaagcgcagt tgttcgcctg
    47341 cgctttcgtt acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg
    47401 tttcaagcag ggcttcgccc acagcgccag ttgcgcccag gacggcaatg ttccagcctt
    47461 cagacatggt ggtttactcc agaaatagca aagctccctg ccaatattac agcagagagc
    47521 atgaagaaga gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa
    47581 tgatgccgcg ctggcgtcat cacaaatcac atacagagat gaccattcac ggcgctcaag
    47641 atagtttttg cgcagcttat cgaactcacc cggtattccg gcgactttac gcagcggtgc
    47701 gtcatcgcgg cgcacatcat acaccaaatg caccagcctt tttagcgtcg gttgatcgag
    47761 cggaccatgc agcgtaatgc ggccaaactc tggcgcgggc agcaacgtat ccagcgcaac
    47821 gtgttgttca tgcccaataa gcttgctata ggcttcaaac acttgcgtag taccgcgtgc
    47881 tttaccttcc agggtatagc ctgcgatatg cggtgtgccg atatccactt ttttcagcaa
    47941 ttccacgttg agttccggtt cgccttccca gacatccagc actacgctta acttctggcc
    48001 ttcgttcaga caggtcaata gggcagtatt atcgacgact gcgccacggc aggcgttaat
    48061 cagaatcgct cctggtttca ggctacggat cagtttttca tccgccagat gcagcgtttt
    48121 gtacggcccg tctttgaaga gtggcgtatg gaaagtcaga atatccgcat gctgaactaa
    48181 ttcatccagc gagcggaaat ccccctcatc cccgcggtcg gcgcgaggcg gatcgcaaag
    48241 caaggttgta atccctaacg cttccagtcg cgcctgtaaa cggcgaccaa cgttaccaac
    48301 gcccacgatc cccactgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag
    48361 cagtgaggag aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa
    48421 accaattccc gcctgcttta accatgcttc atcgacatgg tcggtccccg cggtggcagt
    48481 gccaacaaat ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg
    48541 caccataagc gcatccgcgt ctgccagttg agcgacagga attggacgcc cgggaaccgc
    48601 ggtcacctca cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag
    48661 gattttcacg tttgtgttac ctgtatgaga cgagagttaa ccagacaagt gtgccataat
    48721 ctcgcggcca ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta
    48781 tttccggccc tcctcctggg caaccaccag gccagggaga taacctgccg tctggcgcgg
    48841 gcaatcagcc tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga
    48901 cctcactgac gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc
    48961 tgtcgggaga ttcaccgctg ctttcgcgtc acttccctgc cgctgagcac aatctggcgc
    49021 aacgtctgct ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg
    49081 agtatttacg tctggggaat aatcgtcagg cggtcacgga ttatatccgt cataactttg
    49141 gtcagacgcc gctgaatcag ctctcaccgg agcaattaaa gaccattctc accctgttgc
    49201 aggaagggaa gatggttatt ccgcaacctc agcagcgcga ggcgaccgac cgtccgttat
    49261 taccggcgga gcacaatgcg ctcaaacagc tggtgaccaa acttgcggcg gcaacgggag
    49321 aacccagcaa gcagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt
    49381 taattccagc gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa
    49441 gccagcaaaa tacgccgacg ctggaatcac tccagatggc gctaaaacaa cctttagatg
    49501 ccagtgaact ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat
    49561 catcgctttc ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta
    49621 aagggattga gccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca
    49681 cgttgcaaaa tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga
    49741 tgctggtctg gctggttcgt tagccccgac gaaatgacag tatcgtgaca ataattccca
    49801 gcaccgcaga gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca
    49861 ttcccgccag cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca
    49921 gtgcggtgcc gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa
    49981 agataagcga acatccggct ccggttaacg ccgcgcccgc taatgcgacc cacgcaccag
    50041 gggcttgcca gagcagcaac aagcccaccg tttctacaag cagagagacg atcgccactt
    50101 tcacgccgcc aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc
    50161 cgccaaacgc ggtaagagta aagcccgcca tcgcccatcc tttactggca aagtagagcg
    50221 aaacgaaagt cccgataacc gcaaaaccaa cgccttgtag tgccagacct aaccctggtt
    50281 tccagataag cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt
    50341 tgcgcactgt gccgttacag gcccacgcca gtaagggtaa tgccattgtg gtgagcgcca
    50401 gtgcggcaaa accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg
    50461 caccgtaaat cgccattccg ttccatgaca tcactttgcc ggaatgtttt ggcccaacga
    50521 tgcctaatcc ccaggtcaga gcgcccgtca gtaactggct ttcaccaaag ccaagaatca
    50581 aacgcccgac gaccaacagg gcaaatttga acggtgcgga gacgggcaaa atcgccgcca
    50641 acagcagcgc gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg
    50701 cgccatattg atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact
    50761 gaatgccgac ggcaataccg accatggtgt tgccatagcc cagttcatga tgaacaaaca
    50821 gcgggataac cggcaacggc agccctacgg tcatgtaggt gagaaaaacc gcaaaagcga
    50881 tgcggaagag cgaaaaattg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc
    50941 attactccag aatgcagcgc aaggcgagga atttccccgt ctcatctctc tggtttcagg
    51001 gttacggtgc gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg
    51061 ggaagtttgc ctggaacagg cggcgattgt caataatgtg aaaaagggaa ccatcaggtt
    51121 cccttttgcg ttagtgtcgg atgccgcatt ggcgcgtaat gtcggatgcg acgctggcgc
    51181 gtcttatccg acctactccg aattaatctt tcagcttgcg cattaccagc gtggcgttgg
    51241 tgccgccgaa gccgaagctg ttagacataa ctgtggtcag ttcgcgatcg gtcgtttcgg
    51301 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    51361 taaaaccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca gcagcgccca
    51421 gagagtgacc ggtcattgct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    51481 cacggatagc tgccagttct ttcacgtcgc caaccggagt cgaagtacca tgagagttca
    51541 ggtagtcgat tggggtatca acaccgtgca tcgccatctt catgcagcgc actgcacctt
    51601 cgccagacgg agcaaccatg tctgcgccat cagaggttgc gccgtagcca acgatttcag
    51661 catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    51721 caccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    51781 gggtgtcgtt gtatttagta gacagcgcac ccatcgcgtc gaattcgcaa gccatttccc
    51841 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    51901 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    51961 cgccgtgaat tttaaacggc gtggcgaggc aggcagaaac gccggatgcc atcgctttgg
    52021 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52081 aaacctggaa acgcggggag ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    52141 tctggtaagc ttccggggag aggccagcat cagcaattgc ctgctccata gaaaggaatg
    52201 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    52261 ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    52321 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    52381 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    52441 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    52501 tcagccaatt aatagagaaa tttgcgcagc ctgacacaca tcgctaagat cgagccaccg
    52561 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    52621 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    52681 ggactggaag agacgcgtta tgtttttctc gggggtaacc atttagaggc acgttttcct
    52741 gaacatccac atcctctgtt tgtggtagca gagagcggct tcggcaccgg attaaacttc
    52801 ctgacgctat ggcaggcatt tgatcagttt cgcgaagcat atccgcaagc gcaattacaa
    52861 cgcttacatt tcattagttt tgagaaattt cccctcgccc gtgcggattt agcgctggcg
    52921 catcaacact ggccggagct ggccccgtgg gcggaacagc ttcaggcgca atggcctttg
    52981 cccttgcccg gttgccatcg tttattgctc gatgaaggcc gcataacgct ggatttatgg
    53041 tttggcgata taaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    53101 gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac gcaaaatctg
    53161 tttaacgcca tggcccggct ggcgcgtccg ggcggcacgc tggcgacctt tacgtctgcc
    53221 ggttttgtcc gccgcggttt gcaggaagcc ggattcacta tgcaaaaacg taagggcttt
    53281 gggcgcaaac gggaaatgct ttgtggggtg atggaactga cattacccct cccctgctcc
    53341 acgccgtggt ttaaccgtac gggcagcagc aaacgggaag cggcgattat cggcggtggt
    53401 attgccagcg cgttgttgtc gctggcgcta ttgcgtcgcg gctggcaggt aacactttat
    53461 tgcgcggatg aggcccccgc actgggtgct tccggcaatc gccagggggc gctgtatccg
    53521 cttttaagta aacacgatga agcgctaaac cgttttttct ctaatgcgtt tacttttgct
    53581 cgtcggtttt acgatttatt gcccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    53641 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcgat ggatttaccc
    53701 acagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    53761 aattgcagcg gtattactta tccgcaaggt ggctggctgt gtccagcaga actgacccgt
    53821 aatgtgctgg aactggcgca acagcagggt ttgcagattc attatcaata tcagttacag
    53881 gatttatccc gtaaggatga cggttggttg ttgaattttg caggagatca gcaagcaaca
    53941 cacagtttag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcatct
    54001 ctgccagtat attcggtggc cggtcaagtc agccatattc cgacaacgcc ggagctggcg
    54061 aaactgaagc aagtgctgtg ctatgacggt tatctcacgc cgcaaaatcc ggcgaatcag
    54121 caccattgta ttggtgccag ttatcatcgc ggcagcgaag agacggcgta cagtgatgaa
    54181 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggtaca gtgggcaaaa
    54241 gaggttgatg tcagtggtaa agaggcgcgc tgcggtgtgc gttgtgctac tcgcgatcat
    54301 ctgccaatgg taggcaacgt gcctgattat gacgctacac tcgtggaata tgcgtcgctg
    54361 gcggagaaga aagatgaagc ggtaagcgcg ccggtttatg acgatctctt tatgtttgct
    54421 gctttaggtt cgcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcacag
    54481 atgagtgaag aaccgattcc gatggatgcc agcacgctgg cggcgttaaa cccgaatcgg
    54541 ttatgggtgc ggaagttgtt gaagggtaaa gcggttaagg cggggtaatc tgctctggca
    54601 ttgtttgtcg gatgcggcgt aaacgcctta tccgacctac gcgttacctg taggcctgat
    54661 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gtcacaactt actgctgtga
    54721 ggcctgctga aacaggtgtt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    54781 ttcaccggca ccaatggctt tttccagact ctggctaacg gttgtatgta ccgcctgagc
    54841 ggagtggtcg tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    54901 cccactggca aacaactcat catcactggc atggtcaacc ataccgtcga ttaatgccag
    54961 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55021 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55081 attttgtagt gctttaatga agcgtgccgg acgttcggga atgccgtttg ccagataatc
    55141 catcacctgc gcatgtaccc gccgttgaaa caccacgcgg tccggttcga aatccccttc
    55201 cagattgtcg cagctaacat taaacggata tcccgctgcc acgcagaaca accagtccag
    55261 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgt gtctgggcat cgcgtccgtc
    55321 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gctttcccgg caatacacca
    55381 gtgcgaaatc tcgtggatgg cgctggcata aaagccatga gcgaagacga ttctgttata
    55441 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    55501 attaaattca tcggcaaagc agctattaaa aatctcaatc aactgctcat agtggtgtgt
    55561 actgttcatt agttcatccc caaccagtgg aggatctcct gtccatggct atcgtaaagt
    55621 aatttggcac tcatcaccgc cgagacgata acgatcatcg ggcggatcag cttttgtcct
    55681 ttgctcaaca ccagtcttga acccatgcgc gcgccgagga actgccccac cagcatcaca
    55741 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    55801 gacgttgcgt taagtaattt ggcgtgagca gtggctttgg cgaggttgaa tccgcacagc
    55861 gtaacgaagg ccagcgcgta aaacgatccg gcagccgggc caaaaaatcc atcgtaaaaa
    55921 ccgacgcagc cccccgccac cagcgcaaat ggcagtccat acatccgacg ctggcgatct
    55981 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaataggc
    56041 aaaatctgcc gtaagacatc ggcctgaaca tactgcacca gtagcgcgcc gctcattgag
    56101 ccgacaaagg tcatggcgat attgagtttt tgatcgctta aactaaccac tttacggcga
    56161 ataaagtaga tagtggcgga aatagagcca ccgcaggctt gcagtttatt ggttgccagc
    56221 gcgttagcgg gagacatccc cgccgccatc aatgccggaa tggtgagtaa cccaccgcca
    56281 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcagc
    56341 agcggggaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    56401 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    56461 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct ggtggcggta
    56521 aaggttgatc ctcacactcc agactatcgg caggacaacg taatcgtaca tgcatatgcg
    56581 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    56641 ggcaaagttg ttgcttaatc gccggattaa caaaaatgcg cgtgacgtct ttatcctggg
    56701 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtcgggaca acgtgtttac
    56761 cgtcgcggga aactaaatct aacgcttgcg ggcgcaagag ttgcgcggag gtccagcgtg
    56821 ttttcggcag ttgcaggaag atatcgacat ccagtccggt ctggtggctg gcgtgaccgc
    56881 cgttgaaacg accaccagca ggcatcccca tatcgccaat cagcaccgta cccatgccca
    56941 gattgctcac ctggcgactc agacgctgga taaacatcac caggtctggg tgaccgaaat
    57001 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57061 caccgatgat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    57121 gggttatttt ttgccacggc gtcgctgcca ggctggcgct actggcaagc agagccagca
    57181 gcgcaatcgc ggttttgttc attttttacc agcgtggaat ttcagtcttc acatcggcat
    57241 tttgcgcccg ctgccgtaac aggtgatcca tcaaaacgat cgccagcatc gcttctgcga
    57301 tcggcaccgc gcggatccca acacaaggat cgtgacggcc tttggtgatc atctcaactt
    57361 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    57421 ccatgtgggc aatgatttgc tgcccgctgc tgataccacc aagaatgccg cccgcatggt
    57481 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccacgc agcgctacca
    57541 cgtcaaaacc atcaccaatt tccacgcctt tcaccgcgtt aatgctcatt agcgcatggg
    57601 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggt acgccactgg
    57661 caacgacggt gactttcgca ccaatagagt cgccttcttt tttcagcgcg cgcatcagtt
    57721 catctaacgc gtcgattttg tccgggtccg ggcagaagaa tggattttgc tcgacctgag
    57781 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcaaccg cgaatctcaa
    57841 tgccaaattt ctcggcgaga tattttttgg caatcgcccc tgctgcaacg cgcatagcgg
    57901 tttcacgggc ggaagaacgt ccaccgccgc gataatcacg caggccgtat ttttgttcgt
    57961 aagtgtaatc ggcatgacct ggacggaaaa cgtccttaat cgcactataa tcctgagaac
    58021 gctggtcggt attttcgatc aacaatccga tgctggtgcc agtagtaaca ccttcaaaaa
    58081 caccggagag aattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    58141 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    58201 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    58261 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    58321 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    58381 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatccgga
    58441 tattgttcca taagatgtac catgctgttg ccgacttcac aaatcagcac gccatcatca
    58501 gcgaggtaat ctgccgcgtt tccgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    58561 gatgccaggc ccagttccgg ctcgtggcga tattcgtttg gcaggtcgga catatcttcc
    58621 gcatcgacat acggcgggtt agtgacaatc aggtcgtact gcactttcgg caagtcgcgg
    58681 aacaaatcgg accgaatagg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    58741 tcagcaaccg ccagcgcgtc gggagagatg tccaccgcgt cgacttccgc ttccgggaag
    58801 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatgtgctgc
    58861 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    58921 ggcaccagca cgcgttcatc gacgtaaaat tcatggccgc agaaccaggc tctgttggtc
    58981 aggtaagcca ccggaatgcg ttcgttgacg cggcggatca cgcgctcaac aatacggtgt
    59041 ttttcgctgg aggtcagacg cgcggtgcgc atgtcttccg gaatatccag cggcaggtag
    59101 agtgaaggca acaccagttg cacggcttca tcccacgggt tatcggtgcc gtggccgtac
    59161 cagatattcg ccgcgctgaa gcggctcacc gaccagcgca acatgtcctg aatggtttgc
    59221 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    59281 caataatctc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    59341 gagtgaggaa aaatacgttt aaaacgatcg attgcgctac gagtcgggta aactgtagga
    59401 aaattagaaa tagagaaaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    59461 cgctgtttcg ccagttgatg gcgggtactc gcaagattaa gcaggacacg attgtccacc
    59521 gaccgcagcg taaaaaaatc agcgaagtac cagtaaagcg gttgattcag gagcaggccg
    59581 atgccagcca ctatttttcg gacgagtttc aaccattatt aaatactgaa ggtccggtga
    59641 aatatgttcg cccggatgtc agccattttg aggcgaagaa gttacgccgt ggcgattatt
    59701 caccggagtt gtttcttgat ttgcacggtc tgacgcagct acaagccaaa caggaactgg
    59761 gggcgttgat tgccgcctgc cgccgtgaac atgtgttttg tgcctgcgtg atgcatgggc
    59821 acgggaagca tattttgaag cagcaaacgc cgctgtggct ggcgcaacat ccacatgtga
    59881 tggcatttca ccaggcacca aaagagtatg gtggtgatgc ggctttgttg gtgttgattg
    59941 aagtggaaga gtggctgccg ccggaattgc cctaataaat attgcggtat aaacgtcttc
    60001 tccgaccagg atactgaccg gagaagaatt aaccctacaa ctcattcaaa atgtactgga
    60061 aacgacaggt ggcatcgatt ggctgctgac tgctggtgtc gatccgtatt tgcatatcat
    60121 gcagttgctt atcggtaatt ggatgtgcta ccggcgttac tgtgactttg ctgtcgtcac
    60181 accccgacgt gctgaatgtg atcatatacg acataaagcg acaataagcg ccacgatcgt
    60241 ccattcggct tccatcacac acccgcccgt cgcgcatcag gtaagtcgag agcgtttgtg
    60301 ttatgactcc ccccgtcgat tgcagcgtta gcatcttgtt atggaggacg tagttctcat
    60361 tccaggagat atcatagggg ccaaccggct ccgttgggca ccgggtttgt tttgctgatg
    60421 gaccaatgta agtataacct ctcgcagcag tactcaccgt gttggtactg accaaccacg
    60481 ggcacccgtg agtttcacca tgcacgatga tatcgacccc cagatcaaca catgaccatc
    60541 catccgtaat ccctgggcaa ttggagttat ccacagtatt actctgatga gtgatgttca
    60601 tcgttggtgc tgatcccggt cccgtccaaa ccgtgttagg gatattcccc aaccacaacg
    60661 tatatccatc ctgtgcatat tcgccataat agaccccttc cggcgtcaag acatcgatac
    60721 gaaagacaac ttttgttgta gccccgttac cccaaaatcc ggtataagtc ttcacatccc
    60781 tgaatgtgac gtccgcccag gcggttgcac tcagtagccc ggtcagcagt aaaaaaaaca
    60841 gtccgctcat ttttttcatg ttaaatatac tcaaacgtcg cagtgaggga ggccgtaaag
    60901 tcacccatcg tcacatcccg cccgcttttt gcctgtagcc aggcattaaa atgcagatca
    60961 ttgttgccgc tgctcagcac aaatgtcgcc ccgctggtat tgttgattag cacagcagtg
    61021 ccgtcggtct tttccatgcc aatccccaca ccttgtgctg tcgaagcggc atccagcgcc
    61081 agaaaaccgg gctgctcgct gtcttccgtt ccggtaagtg tcacccgaac ctcat
//
LOCUS       DAJOTB010000009        60160 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJOTB010000009 DAJOTB010000000
VERSION     DAJOTB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.009"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..751
                     /gene="ycaL"
                     /locus_tag="OHN28_000597"
     CDS             5..751
                     /gene="ycaL"
                     /locus_tag="OHN28_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCQ2296043.1"
                     /translation="MLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATLSDADAK
                     AIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            924..1607
                     /gene="cmk"
                     /locus_tag="OHN28_000598"
     CDS             924..1607
                     /gene="cmk"
                     /locus_tag="OHN28_000598"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCQ2296044.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1718..3391
                     /gene="rpsA"
                     /locus_tag="OHN28_000599"
     CDS             1718..3391
                     /gene="rpsA"
                     /locus_tag="OHN28_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCQ2296045.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3551..3835
                     /gene="ihfB"
                     /locus_tag="OHN28_000600"
     CDS             3551..3835
                     /gene="ihfB"
                     /locus_tag="OHN28_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCQ2296046.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4041..6305
                     /locus_tag="OHN28_000601"
     CDS             4041..6305
                     /locus_tag="OHN28_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCQ2296047.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6342..8090
                     /gene="msbA"
                     /locus_tag="OHN28_000602"
     CDS             6342..8090
                     /gene="msbA"
                     /locus_tag="OHN28_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCQ2296048.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8087..9073
                     /gene="lpxK"
                     /locus_tag="OHN28_000603"
     CDS             8087..9073
                     /gene="lpxK"
                     /locus_tag="OHN28_000603"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCQ2296049.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            9110..10342
                     /locus_tag="OHN28_000604"
     CDS             9110..10342
                     /locus_tag="OHN28_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCQ2296050.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRYLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            10394..10576
                     /gene="ycaR"
                     /locus_tag="OHN28_000605"
     CDS             10394..10576
                     /gene="ycaR"
                     /locus_tag="OHN28_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCQ2296051.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            10573..11319
                     /gene="kdsB"
                     /locus_tag="OHN28_000606"
     CDS             10573..11319
                     /gene="kdsB"
                     /locus_tag="OHN28_000606"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCQ2296052.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11473..12366
                     /locus_tag="OHN28_000607"
     CDS             11473..12366
                     /locus_tag="OHN28_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCQ2296053.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(12343..13122)
                     /gene="elyC"
                     /locus_tag="OHN28_000608"
     CDS             complement(12343..13122)
                     /gene="elyC"
                     /locus_tag="OHN28_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCQ2296054.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13258..14043
                     /gene="cmoM"
                     /locus_tag="OHN28_000609"
     CDS             13258..14043
                     /gene="cmoM"
                     /locus_tag="OHN28_000609"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCQ2296055.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14040..15362
                     /gene="mukF"
                     /locus_tag="OHN28_000610"
     CDS             14040..15362
                     /gene="mukF"
                     /locus_tag="OHN28_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCQ2296056.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15343..16047
                     /gene="mukE"
                     /locus_tag="OHN28_000611"
     CDS             15343..16047
                     /gene="mukE"
                     /locus_tag="OHN28_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCQ2296057.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16047..20507
                     /gene="mukB"
                     /locus_tag="OHN28_000612"
     CDS             16047..20507
                     /gene="mukB"
                     /locus_tag="OHN28_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCQ2296058.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20768..22615
                     /gene="ldtD"
                     /locus_tag="OHN28_000613"
     CDS             20768..22615
                     /gene="ldtD"
                     /locus_tag="OHN28_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2296059.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22796..23344
                     /locus_tag="OHN28_000614"
     CDS             22796..23344
                     /locus_tag="OHN28_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCQ2296060.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23371..24018
                     /gene="gloC"
                     /locus_tag="OHN28_000615"
     CDS             23371..24018
                     /gene="gloC"
                     /locus_tag="OHN28_000615"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCQ2296061.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24068..25258)
                     /gene="aspC"
                     /locus_tag="OHN28_000616"
     CDS             complement(24068..25258)
                     /gene="aspC"
                     /locus_tag="OHN28_000616"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCQ2296062.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25443..26531)
                     /gene="ompF"
                     /locus_tag="OHN28_000617"
     CDS             complement(25443..26531)
                     /gene="ompF"
                     /locus_tag="OHN28_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCQ2296063.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(27134..28534)
                     /gene="asnS"
                     /locus_tag="OHN28_000618"
     CDS             complement(27134..28534)
                     /gene="asnS"
                     /locus_tag="OHN28_000618"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCQ2296064.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(28703..29905)
                     /gene="pncB"
                     /locus_tag="OHN28_000619"
     CDS             complement(28703..29905)
                     /gene="pncB"
                     /locus_tag="OHN28_000619"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCQ2296065.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            30171..32783
                     /gene="pepN"
                     /locus_tag="OHN28_000620"
     CDS             30171..32783
                     /gene="pepN"
                     /locus_tag="OHN28_000620"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCQ2296066.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(32826..33593)
                     /gene="ssuB"
                     /locus_tag="OHN28_000621"
     CDS             complement(32826..33593)
                     /gene="ssuB"
                     /locus_tag="OHN28_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2296067.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(33590..34381)
                     /gene="ssuC"
                     /locus_tag="OHN28_000622"
     CDS             complement(33590..34381)
                     /gene="ssuC"
                     /locus_tag="OHN28_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCQ2296068.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(34393..35538)
                     /gene="ssuD"
                     /locus_tag="OHN28_000623"
     CDS             complement(34393..35538)
                     /gene="ssuD"
                     /locus_tag="OHN28_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCQ2296069.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(35535..36494)
                     /gene="ssuA"
                     /locus_tag="OHN28_000624"
     CDS             complement(35535..36494)
                     /gene="ssuA"
                     /locus_tag="OHN28_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCQ2296070.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(36487..37062)
                     /gene="ssuE"
                     /locus_tag="OHN28_000625"
     CDS             complement(36487..37062)
                     /gene="ssuE"
                     /locus_tag="OHN28_000625"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCQ2296071.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            37299..38309
                     /gene="pyrD"
                     /locus_tag="OHN28_000626"
     CDS             37299..38309
                     /gene="pyrD"
                     /locus_tag="OHN28_000626"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCQ2296072.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            38483..39025
                     /gene="zapC"
                     /locus_tag="OHN28_000627"
     CDS             38483..39025
                     /gene="zapC"
                     /locus_tag="OHN28_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCQ2296073.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(39022..40131)
                     /gene="ycbX"
                     /locus_tag="OHN28_000628"
     CDS             complement(39022..40131)
                     /gene="ycbX"
                     /locus_tag="OHN28_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCQ2296074.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            40229..42337
                     /gene="rlmKL"
                     /locus_tag="OHN28_000629"
     CDS             40229..42337
                     /gene="rlmKL"
                     /locus_tag="OHN28_000629"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCQ2296075.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            42349..44256
                     /locus_tag="OHN28_000630"
     CDS             42349..44256
                     /locus_tag="OHN28_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2296076.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            44386..45639
                     /gene="pqiA"
                     /locus_tag="OHN28_000631"
     CDS             44386..45639
                     /gene="pqiA"
                     /locus_tag="OHN28_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCQ2296077.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            45644..47284
                     /gene="pqiB"
                     /locus_tag="OHN28_000632"
     CDS             45644..47284
                     /gene="pqiB"
                     /locus_tag="OHN28_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCQ2296078.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            47281..47844
                     /gene="pqiC"
                     /locus_tag="OHN28_000633"
     CDS             47281..47844
                     /gene="pqiC"
                     /locus_tag="OHN28_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCQ2296079.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            48100..48267
                     /gene="rmf"
                     /locus_tag="OHN28_000634"
     CDS             48100..48267
                     /gene="rmf"
                     /locus_tag="OHN28_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCQ2296080.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(48337..48855)
                     /gene="fabA"
                     /locus_tag="OHN28_000635"
     CDS             complement(48337..48855)
                     /gene="fabA"
                     /locus_tag="OHN28_000635"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCQ2296081.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(48924..50684)
                     /locus_tag="OHN28_000636"
     CDS             complement(48924..50684)
                     /locus_tag="OHN28_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCQ2296082.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            50870..51322
                     /gene="matP"
                     /locus_tag="OHN28_000637"
     CDS             50870..51322
                     /gene="matP"
                     /locus_tag="OHN28_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCQ2296083.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(51398..52450)
                     /gene="ompA"
                     /locus_tag="OHN28_000638"
     CDS             complement(51398..52450)
                     /gene="ompA"
                     /locus_tag="OHN28_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCQ2296084.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(52806..53315)
                     /gene="sulA"
                     /locus_tag="OHN28_000639"
     CDS             complement(52806..53315)
                     /gene="sulA"
                     /locus_tag="OHN28_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCQ2296085.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            53534..54163
                     /gene="sxy"
                     /locus_tag="OHN28_000640"
     CDS             53534..54163
                     /gene="sxy"
                     /locus_tag="OHN28_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCQ2296086.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSQTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(54126..56288)
                     /gene="yccS"
                     /locus_tag="OHN28_000641"
     CDS             complement(54126..56288)
                     /gene="yccS"
                     /locus_tag="OHN28_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCQ2296087.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(56298..56744)
                     /locus_tag="OHN28_000642"
     CDS             complement(56298..56744)
                     /locus_tag="OHN28_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCQ2296088.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            56867..58921
                     /gene="helD"
                     /locus_tag="OHN28_000643"
     CDS             56867..58921
                     /gene="helD"
                     /locus_tag="OHN28_000643"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCQ2296089.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(58953..59411)
                     /gene="mgsA"
                     /locus_tag="OHN28_000644"
     CDS             complement(58953..59411)
                     /gene="mgsA"
                     /locus_tag="OHN28_000644"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCQ2296090.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(59507..60157)
                     /locus_tag="OHN28_000645"
     CDS             complement(59507..60157)
                     /locus_tag="OHN28_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCQ2296091.1"
                     /translation="MVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLLRGAD
                     SIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLENERE
                     ANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASLPQFA
                     TMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQPSS"
BASE COUNT        14960 a        14821 c        16014 g        14365 t
ORIGIN      
        1 attattgctg gcgattgcga cctctgcagc cttactgaca ggatgtcaga atacgcacgg
       61 tattaatacc gatttggcta tcagctcagg tctgaatgct tataaagccg caacattaag
      121 cgatgctgat gctaaagcaa tcgccaatca gggctgtgtc gaaatggaca gcggcaataa
      181 agtggcaagc aaatccagca agtacgggaa acgtctggca aaaatcgcca aagcattggg
      241 taacaatatt aatggcacgc cagtcaacta taaggtttat atgaccagcg acgtcaacgc
      301 atgggcgatg gcgaacggtt gtgttcgtgt ctacagtggc ctgatggaca tgatgaacga
      361 caacgaaatt gaaggcgttc tgggccatga actgggccac gtcgcgttgg gtcactcgct
      421 ggctgaaatg aaagcttctt atgcgatcgt ggccgcacgc gatgccattt cagctaccag
      481 cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag gcgctatcaa
      541 tgctaaatac tcccgtgata aagagtcaga agcagatgat ttctcctttg atctgttgaa
      601 gaaacgtggc atcagtaccc aggggctggt tggtagcttt gaaaaactcg ctagcctgga
      661 tggcggtcac acccagtcca tgtttgattc tcacccacca tcaacagagc gtgcgcaaca
      721 catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg ctggtcttct
      781 gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa ctggtttttg
      841 cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt
      901 acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc attgatggcc
      961 caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg caatggcatc
     1021 tgctggattc gggtgcaatt tatcgcgtac tggcactagc ggcattacat caccatgttg
     1081 atgttgcatc ggaagatgcg ctggtgccgc tggcatccca tctggatgta cgttttgtgt
     1141 caaccaacgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta
     1201 ctcaggaggt ggcgaatgct gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat
     1261 tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat gggcgcgaca
     1321 tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag
     1381 aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt aactttgagc
     1441 gccttttggc cgagatcaaa gaacgtgacg accgcgatcg taatcgagcg gtagcgccac
     1501 tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt gagcaagtga
     1561 ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg accgaatttg
     1621 cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat ccggcatgga
     1681 gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt ttgctcaact
     1741 ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt
     1801 tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg agtccgccat
     1861 cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag gtgacgaagt
     1921 tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt cccgtgagaa
     1981 agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt
     2041 taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga acggtattcg
     2101 tgcgttcctg ccaggttctc tggtagatgt tcgtccggtg cgtgacactc tgcacctgga
     2161 aggcaaagag cttgaattca aagtaatcaa gctggatcag aagcgcaaca acgttgttgt
     2221 ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa
     2281 cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact acggtgcatt
     2341 cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct ggaaacgcgt
     2401 taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt
     2461 cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag atccgtgggt
     2521 agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga ccaacctgac
     2581 cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac acgtttccga
     2641 aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg gcgatgtagt
     2701 ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca
     2761 gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg accgtgttga
     2821 aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg gcggcatcga
     2881 cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag cagttcgtga
     2941 atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg
     3001 tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg ttgctctgaa
     3061 caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt
     3121 agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt
     3181 tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt
     3241 tgatcgtaaa aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa
     3301 agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca acgcaatggc
     3361 tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat ttttattccg
     3421 aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag cactaagggc
     3481 ggctacggcc gcccttaatc aatgtagcaa cagcagccgc ttaatttgcc tttaaggaac
     3541 cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc aatcgcacat
     3601 tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg cctcgactct
     3661 tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc actaccgcgc
     3721 accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag gaaaatacgt
     3781 tcctcacttt aaaccaggta aagaactgcg cgatcgcgcc aatatttacg gttaagtttt
     3841 tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcattt ctctaatctg
     3901 gaactggaag ctgcctcgca gagttttgag cagttttcac cctatcgtta aattcttctg
     3961 aacgtgcctc ggggaacgca aaactcccac cagacaaccg ttcaacaaag ttgcacactt
     4021 tctataaaca gggagggtgc atgaaaataa cgacagtcgg cgtatgcata atttgcggaa
     4081 tttttccgtt gctgattttg ccccaattgc cagggacagt aacccttgcg tttctgactc
     4141 tcttcgcctg tgtactggca tttatccctg ttaaaaccgt ccgttatatc gcgctgacgt
     4201 tgctgttttt cgtttggggc atattagcag caaagcaaat tttgtgggca ggagaaacct
     4261 taactggcgc gacgcaggat gcaattgttg agatcattgc tactgacggc atgaccactc
     4321 attacggtca aattactcat ctacaaggtc gacgtatatt ccctgcgcca ggcctcgtac
     4381 tgtatggcga atatcttccg caagcggttt gtgccggaca agtatggtca atgaaactca
     4441 aagttcgtgc agttcatggt caacttaatg atggcggctt tgatagccag cgttatgcca
     4501 ttgcccagca tcaaccgctc accggccgtt ttctgcaggc aagtgtcatt gaaccgaatt
     4561 gtagcctgcg tgcacagtat ctggcgtcac tacaaacaac gctgcaaccc tatatgtgga
     4621 acgcggttat tcttggatta ggtatggggg aacggttatc cgtccccaaa gaaatcaaaa
     4681 atatcatgcg tgatactgga acggcgcatt taatggcgat atcaggattg cacatcgctt
     4741 ttgcggcgtt gctggctgca ggactcattc gcggtgggca agtttttctg cctgggtgct
     4801 ggctccactg gcaaatgcca ttaattggtg gaatctgctg tgctgctttt tatgcctggc
     4861 ttacgggaat gcaacctcct gcattgcgta ccgtggtggc gcttgccatt tggggaatgc
     4921 ttaagttaag tggacgacaa tggagtggct gggatgtatg gatatgttgt ctggcggcaa
     4981 ttttgctgat ggatcctgtt gccattctct cgcaaagttt atggctttct gccgctgcgg
     5041 tcgcggcact gattttttgg tatcagtggt ttccctgtcc ggagtggcag ctgccgccgg
     5101 tattgcgcgc ggttgtttcc ctcatccatc tgcaactggg aatcaccctc ctgcttatgc
     5161 ccgtgcaaat tgtcatattt catggcatta gtctgacctc gtttattgca aatctttttg
     5221 caattcccct ggtgacattt atcacggttc cgttgattct cgccgcgatg gttgtgcatt
     5281 taagcgggcc gtttattctg gaacaagggt tatggtttct tgccgaccgg tctttggctt
     5341 tacttttctg ggggttaaag agtttgccgg aagggtggat caacattgct gaacgttggc
     5401 aatggctaac attttcccca tggttcttac tggtggtatg gcgattaaac gcctggcgaa
     5461 cgttgccagc aatgtgtgtg gctgtaggct tgctgatgtg cttgccgctg tggcaaaaac
     5521 ctcgacctga cgagtggcaa gtgtacatgc ttgatgtcgg gcaggggctg gcaatggtca
     5581 ttgcgcgaaa cggcaaagcg attctctatg acacgggact ggcctggccc gaaggggata
     5641 gtgggcaaca actgattatc ccctggctcc actggcataa tcttgaaccg gaaggcgtta
     5701 ttttgagtca tgaacatctg gatcaccggg gagggctgga ctcaatattg cacacatggc
     5761 cgatgttatg gatccgaagt ccgttaaact gggaacatca tcagccctgt gtgcgtggcg
     5821 aagcgtggca atggcaagga ttgcgtttca gcgcgcactg gcctttacaa ggtagcaacg
     5881 ataaaggaaa taaccattcc tgtgtggtta agattgatga cgggacgaat agcattcttc
     5941 taaccggtga tattgaagcc cccgctgaac aaaagatgct aagccgttac tggcagcaaa
     6001 tgcaggcaac attacttcag gtacctcacc atggcagtaa tacctcatca tcgttgccat
     6061 taattcagcg agtgaatgga aaagtggcac tcgcatcggc atcgcgctat aacgcatggc
     6121 gactgccctc aagcaaagtt aaatatcgct atcatcaaca gggttataag tggcttgata
     6181 ctccacatca ggggcaaata acggtcaatt tttcagcgca aggctggtgg attagcagcc
     6241 tcagagagca aattttacct cgttggtatc atcagtggtt tggcgtgcca gtggataacg
     6301 ggtagaatat gcggctattt caacaaatgc tggttttttg aatgcataac gacaaagatc
     6361 tctctacgtg gcagacgttc cgccgactgt ggccaaccat tgcgcctttt aaagcgggtc
     6421 tgatcgtggc gggcgtagcg ttaatcctca acgcagccag cgataccttc atgttatcgc
     6481 tccttaagcc acttcttgat gatggctttg gtaaaacaga tcgctccgtg ctgatgtgga
     6541 tgccgctggt ggtgatcggg ctgatgattt tacgtggtat caccagctat atctccagct
     6601 actgtatctc ctgggtatca ggaaaggtgg taatgactat gcgtcgccgc ctgtttggtc
     6661 acatgatggg aatgccggtt tcattctttg acaaacagtc aacgggtacg ctgttgtcac
     6721 gtatcaccta cgattccgaa caggtcgctt cttcttcttc cggcgcactg attactgttg
     6781 tgcgtgaagg tgcgtcgatc atcggcctgt tcatcatgat gttctattac agttggcaac
     6841 tgtcgatcat tttgattgtg ctggcaccga ttgtttcgat tgcgattcgc gttgtatcga
     6901 agcgttttcg caacatcagt aaaaacatgc agaacaccat ggggcaggtg accaccagcg
     6961 ccgaacaaat gctgaaaggc cataaagaag tattgatttt cggtggtcag gaagtggaaa
     7021 cgaaacgctt cgataaagtc agcaaccgaa tgcgtcttca ggggatgaaa atggtttcag
     7081 cctcttccat ctctgatccg atcattcagc tgatcgcctc tttggcgctg gcgtttgttc
     7141 tgtatgcggc aagcttccca agtgtcatgg atagcctgac tgccggtacg attaccgttg
     7201 ttttctcttc aatgattgca cttatgcgtc cgctgaaatc gctgaccaac gttaacgccc
     7261 agttccagcg cggtatggcg gcttgtcaga cgctgtttac cattctggac agtgagcagg
     7321 agaaagacga aggtaagcgc gtgatcgagc gtgcgactgg cgacgtggag tttcgcaatg
     7381 tcacctttac ttatccggga cgtgatgtgc ctgcattgcg taacatcaac ctgaaaattc
     7441 cggcagggaa gacggttgct ctggttggcc gctctggttc aggtaaatca accatcgcca
     7501 gcctaatcac acgtttttac gatattgatg aaggcgaaat cctgatggat ggtcacgatc
     7561 tgcgcgagta taccctggcg tcgttacgta accaggttgc tctggtgtcg cagaatgtcc
     7621 atctgtttaa cgatacggtt gccaacaaca ttgcttacgc aaggactgaa cagtacagcc
     7681 gtgagcaaat tgaagaagct gcgcgtatgg cctacgccat ggacttcatc aataagatgg
     7741 ataacggtct cgatacagtc attggtgaaa acggcgtgtt gctctctggc ggtcagcgtc
     7801 agcgtattgc tatcgctcga gccttgttgc gtgatagccc gatactgatt ctggacgaag
     7861 ctacctcggc gttggatacc gaatccgaac gtgcgattca ggcagcactg gatgagttgc
     7921 agaaaaaccg tacctctctg gtgattgccc accgcttgtc taccattgaa aaggcagacg
     7981 aaatcgtggt tgtcgaggat ggtgtcattg tggaacgcgg tacgcataac gatttgcttg
     8041 agcaccgtgg cgtttacgcg caacttcaca aaatgcagtt tggccaatga tcgaaaaaat
     8101 ctggtctggt gagtcccctt tatggcggct attgctgcca ctctcctggt tgtatggcct
     8161 ggtgagtggc gcgatccgtc tttgctataa actaaaactg aagcgcgcct ggcgtgcccc
     8221 cgtaccggtt gtcgtggttg gaaatctcac tgcaggcggc aacggaaaaa ccccggtcgt
     8281 tgtctggctg gtggaacagc tgcaacagcg cggtattcgc gtgggggtcg tatcgcgggg
     8341 atatggtggt aaggctgaat cttatccgct gttattgtcg gcagatacca ccacagcaca
     8401 ggcgggtgat gagcctgtgt tgatttatca acgcactggc gcgcctgttg cggtttctcc
     8461 cgtgcgttct gacgcggtaa aagccattct ggcgcaacac cctgatgtgc agatcatcgt
     8521 aactgacgat ggtttacagc attatcgtct ggcgcgtgat gtggaaattg tcgttattga
     8581 tggtgtgcgt cgctttggca atggctggtg gttgccggcg gggccaatgc gtgagcgagc
     8641 agggcgctta aagttggttg atgcggtaat cgtcaacggc ggtgtccccc gcagcggtga
     8701 aatccccatg catctgctgc cgggtcaggc ggtgaattta cgtaccggta cacgttgtga
     8761 cgttgctcag cttgaacatg tggtggcgat tgcggggatt gggcatccgc cgcgcttttt
     8821 tgccacgctg aagatgtgtg gcgtacaacc cgaaaaatgt gtaccgctgg ccgatcatca
     8881 gtttttgaat catgcggatg tcagcgcgtt ggtaagcgcc ggacaaacgc tggtaatgac
     8941 tgaaaaagat gcggtgaaat gccgggcctt tgcagaagaa aattggtggt atttgcctgt
     9001 agacgcacag ctttcaggtg atgaaccagc gaaactgctt gcgcaactaa cttcgctggc
     9061 ttctggcaac tagttacgcc gcggcagcgt tcgattgatg gagtcatgaa tgtctctgcc
     9121 gcacctctcc cttgctgatg cgcgtaatct tcatctcgct gcacaaggcc tgttaaacaa
     9181 accccaccgt cgagcgtcgt tggaggatat tccggcaacg atctcccgca tgtccttgct
     9241 gcaaatcgat accatcaata ttgttgcccg tagcccatat ctggtgcttt tcagtcgtct
     9301 gggaaattat cctgcccagt ggctggatga gtctctggcg cgtggcgaat taatggaata
     9361 ctgggcgcat gaagcctgct ttatgccgcg tagcgatttt cgtcttattc gccaccgcat
     9421 gctggcacct gaaaaaatgg gctggaaata taaagacgcc tggatgcagg aacatgcggc
     9481 ggaaattgca ctgttaattc agcatattca tgataggggg ccggtacgtt cagccgattt
     9541 tgagcatcct cgtaaaggtg caagcggctg gtgggagtgg aagccgcata aacgatatct
     9601 ggaaggttta tttactgccg gaaaggtgat ggtgattgaa cggcgcaact tccagcgcgt
     9661 ttatgattta acccaccgtg tcatgcctga ctgggatgat gagcgcgatc tcgtttcgca
     9721 agcagaagca gaaatcatca tgctggataa cagtgcgcgt agcctgggaa tatttcgcga
     9781 acagtggctg gcagattact accggctaaa acgtccggca ctggcggcgt ggcgtgaagc
     9841 gagggctgaa cagcagcaaa tcattgctgt gcatgttgaa aaattgggca atctttggct
     9901 gcatgctgat ttgctgccgc tactcgagcg tgcgctcgcc ggaaagctca ctgcaacgca
     9961 cagcgcggta ctttcgcctt ttgatcctgt tgtctgggat cgcaaacgcg cggagcagct
    10021 ttttgatttt agctaccggc tggagtgcta taccccagcg ccgaaacgcc agtatggcta
    10081 ttttgttctg ccgttattac atcgtgggca attagttggg cgaatggatt ccaaaatgca
    10141 tcgccagaaa ggcatccttg aagttatctc tctgtggtta caggaaggca ttaaaccaac
    10201 gacaacgctg caaaaagggt tacgtcaggc gattactgat ttcgctaact ggcagcaggc
    10261 aacgcgggtg acattaggac gctgctcgca aggcctcttt actgattgcc gcgccggctg
    10321 ggaaatagac cccgtcgcat aaatgattat gataagattt atgcattcat aacccgatcc
    10381 atctggagga actatggatc atcgtctgct tgaaatcatt gcctgcccgg tttgcaacgg
    10441 aaaactttgg tataaccagg aaaaacaaga gcttatttgc aaactggaca acctggcttt
    10501 cccgctgcgc gatggcattc cagtgttgct ggaaacggaa gcccgcgtgc tgactgctga
    10561 tgagggtaaa tcatgagttt tgtggtcatt attcccgcgc gttacgcgtc cacgcgtctg
    10621 cccggtaaac cattggttga tattaacggc aaacccatga ttgttcatgt tcttgaacgc
    10681 gcgcgcgaat caggtgccga gcgcatcatc gtggcaaccg atcatgagga tgttgcccgc
    10741 gctgttgaag ccgctggcgg cgaagtatgt atgacgcgcg ccgatcatca gtcaggtaca
    10801 gaacgtttgg cggaagttgt cgaaaaatgc gcattcagcg acgacacggt gatcgttaat
    10861 gtgcagggtg atgaaccgat gatccctgcg acaatcattc gtcaggttgc tgataacctc
    10921 gctcagcgtc aggtgggtat ggcgactctg gcggtgccaa tccacaatgc ggaagaagcg
    10981 tttaacccga atgcggtgaa agtggttctc gatgccgaag ggtacgcact gtacttctct
    11041 cgcgccacca ttccgtggga tcgtgatcgt tttgcaaaag accttgaaac cgttggcgat
    11101 aacttcctgc gtcatcttgg tatttatggc taccgagcag gctttatccg tcgttatgtt
    11161 acctggcagc caagtccgtt agaacatatc gaaatgttag agcagcttcg tgttctgtgg
    11221 tatggcgaaa aaatccacgt tgctgtcgcc caggaagttc ctggcacagg tgtggatacc
    11281 cctgaagatc tcgagcgcgt tcgcgctgaa atgcgctaat ttcacttcac gacactccag
    11341 ccaattttgg gaggagtgtc gtaccgttac gattttcctc aatttttctt ttcaacaatt
    11401 gatctcattc aggtgacatc ttttatattg gcgctcatta tgaaagcagt agcttttatg
    11461 agggtaatct gaatggaaca gctgcgtgcc gaattaagcc atttactggg cgaaaaactc
    11521 agtcgtattg agtgcgtcaa tgaaaaagcg gatacggcgt tgtgggcttt gtatgacagc
    11581 cagggaaacc caatgccgtt aatggcaaga agctttagta cgcccggaaa agcccgacaa
    11641 ctggcatgga aaaccaccat gctggcaaga agtgggactg tccgtatgcc gactatttat
    11701 ggtgtgatga cgcatgaaga acaccccggc cccgatgtcc tgctactgga gcgaatgcgt
    11761 ggtgtttcgg tggaggcacc agcccgaaca ccagaacgct gggaacaact caaagaccaa
    11821 atcgttgaag ccttactggc ctggcaccgt caggacagtc gtggttgcgt cggcgcggtc
    11881 gacaatactc aggaaaattt ctggccctca tggtaccggc aacatgttga agtgctatgg
    11941 accacgctca atcagttcaa taacaccggt ctatcgatgc aggataagcg gatcctgttt
    12001 cgcactcgcg aatgtctccc ggcattattt gaaggcttta acgacaattg tgtactgatt
    12061 cacggtaact tctgtttacg cagcatgctg aaagattcgc gcagcgatca gttactggcg
    12121 atggtcggac cgggactaat gctttgggca ccgcgagaat acgaactgtt ccgactaatg
    12181 gataattctc tggcggaaga tttgctctgg agttatctgc aacgcgcgcc agtggcggag
    12241 tcgttcatct ggcggcgttg gttgtatgtg ttatgggatg aagttgcgca actggtcaac
    12301 accggacgat ttaatcggcg taacttcgat ctggcatcaa aatcactctt gccgtggctc
    12361 gcctgacgaa cctttcagcc actgccagat ccggccaagc gtttcatagc caacgcggtc
    12421 gctatgcatc aaccaaaccg gagaagggat tgcccgttcc cacggattaa gcggcgagtc
    12481 gatggccaac tgattcgccg gggctggtag cggatttaac ccttcctgct gaaaaaagat
    12541 catcgcccgc ggcagatgtg atgcggaggt caccagcagg aaaggtgcgt caccaatcgc
    12601 ctgtttcact gctgcagctt cttcttcggt atcttttggc aaatccaggg tgataatttg
    12661 ctcgcgcggc acgcccagcg attgcgcaac tctggcaccg acttcggctg tgctcaccgt
    12721 attggtttta gctacgcctc ccgtgaagat cagttttgat cccggatttt cgcgccataa
    12781 gcgaatacct tcattcaggc gtggcagact gttattgatt aaattagagc tcggcgccca
    12841 ctgcgggttc caggtataac cacctccgag caccacgata tagtccactt tttgcgaatt
    12901 attccatgtc ggataggtgc tttcgatggg acgcagcagg cggtcggcta ccggttgcaa
    12961 actcaataac aagagcgcca gccacccaat gctaataaaa atcttcccgg ttttttgaaa
    13021 acggctaaac cacagcagcg ccaggccagc gccaataatc aacaacatta gcggaagtgg
    13081 cagcaacata ttgccaatca cttttttcag tgtaaaaagc atcctttctg gttccttttt
    13141 taaccatata gcaagggatc gccagcgata ttacaccaga cgggttcatt ctcgccgtgg
    13201 ctgtgacaaa atagcgtctt tatcagaaaa cgtcttagcc aaccggtgga gaaagcaatg
    13261 caggatcgca attttgatga tattgcggaa aagttttccc gtaacattta cggcaccacc
    13321 aaagggcagc ttcgacaggc tattctgtgg caggatctcg atcgcgtgct ggcagaaata
    13381 ggcccgcaaa aactgcgtgt gctggatgct ggcggtggag aagggcagac cgcaatcaaa
    13441 atggccgagc gcgggcatca ggtcatttta tgcgatcttt ctgcgcagat gatcgaccgc
    13501 gcaaaacagg cggcagaagc aaaaggtgtg agcgacaaca tgcaatttat acattgcgcc
    13561 gctcaggatg ttgcttcgca tttggaaacg cccgttgatc tgatattgtt ccatgcggtg
    13621 ctcgagtggg tggccgatcc ccgcagcgta ttacagaccc tgtggtcagt gttgcgtcca
    13681 ggcggcgtgt tgtcgttaat gttctacaat gcgcatggtt tgttgatgca taacatggtc
    13741 gccgggaatt ttgattacgt gcaggcggga atgccgaaaa agaaaaaacg gacgctttcg
    13801 ccagattatc cacgcgaccc ggcgcaggtt tatctgtggc tggaagaagc tggttggcaa
    13861 attctgggta agacaggcgt tcgcgtgttt catgattatc tgcgtgagaa gcaccagcag
    13921 cgcgactgct atgaagcatt acttgaatta gaaacgcgtt attgccgtca ggaaccgtat
    13981 attaccctgg ggcgttatat tcatgtcacc gcgcgcaaac cgcagagcaa ggataaagta
    14041 tgagtgaatt ttcccagaca gtccccgaac tggttgcctg ggccagaaaa aatgacttct
    14101 ccatctcgct gccggtagac cgactctctt ttctgctggc ggttgccacg ctgaacggcg
    14161 agcgtctgga tggtgagatg agtgaaggcg agctggtgga tgcattccgc catgtgagtg
    14221 atgcgtttga gcaaaccagc gaaaccatcg gcgtgcgcgc caacaacgcg atcaacgaca
    14281 tggtgcgtca acgtctgctg aaccgcttta ccagcgagca ggcggaaggg aacgcaattt
    14341 accgtctgac gccgctcggc atcggcatca ctgactatta cattcgtcag cgcgagtttt
    14401 ctacgctgcg cctttctatg caattgtcga ttgtggcggg tgagctcaaa cgcgcagcag
    14461 atgccgccga agagggcggt gatgaatttc actggcaccg caatgtttat gcgccactga
    14521 aatattcggt agcagaaatt ttcgacagta tcgacctgac gcaacgtctg atggatgaac
    14581 agcagcagca ggtgaaggat gatatcgccc agttgttgaa caaagactgg cgggcggcga
    14641 tttccagctg tgaattgttg ctttcagaaa cttccggaac gctgcgtgaa ttgcaggata
    14701 cgctggaagc ggcaggcgat aaattgcagg ccaacctgtt acgcattcag gatgcgacga
    14761 tgacccatga cgacctgcat tttgtcgatc gtctggtgtt cgatcttcag agcaaactcg
    14821 accgtattat cagttggggc cagcaatcca tcgacttgtg gattggctac gaccgccacg
    14881 tacacaaatt tattcgtact gcgatcgata tggataaaaa ccgcgtcttt gctcagcggt
    14941 tacgtcagtc ggtacaaacc tattttgatg agccgtgggc gctaacttat gccaatgccg
    15001 atcgtctgct ggatatgcgt gacgaagaga tggtactgcg cgatgaagaa gtgactgggg
    15061 aacttcctga ggatctggag tacgaagagt ttaacgagat ccgcgaacag ctggcggcga
    15121 tcatcgaaga acaacttgcc gtgtacaaaa ccagacaagt gccgttggat cttggtctgg
    15181 tggtacgcga atatctgtca cagtatccgc gtgcacgtca ctttgacgtt gcgcgcattg
    15241 ttattgatca ggcggtacgt cttggcgtag cgcaagcaga tttcaccgga ctgccagcga
    15301 aatggcagcc gattaatgat tacggagcca aggtacaggc gcatgtcatc gacaaatatt
    15361 gaacaagtga tgccggttaa gctggcgcag gcgctggcga atccgttatt tccggcgctg
    15421 gatagcgcct tacgttcagg ccgccatatt ggcctcgacg aactggataa tcatgcattc
    15481 ctgatggatt ttcaggaata tctggaagag ttttacgcgc gttataacgt tgagcttatt
    15541 cgtgcaccgg aagggttctt ctatctacgc ccacgttcca ccacgctgat ccctcgttcc
    15601 gtcttgtcgg aactggatat gatggtcggg aaaatcctct gttatctcta tctcagcccg
    15661 gaacggctgg cgaatgaggg gatttttact caacaggaac tgtacgacga actgctcacc
    15721 ctggccgatg aagcgaaact gctgaaactg gtgaacaacc gttcaaccgg ttcagacgtt
    15781 gaccgtcaga agttgcagga aaaagtacgt tcttcgctca accgtctgcg tcgtttaggc
    15841 atggtgtggt ttatgggcca cgacagcagc aagttccgca ttaccgaatc ggtgttccgc
    15901 ttcggcgccg atgtgcgtgc tggcgacgat ccccgtgaag cacagcgtcg cctgattcgt
    15961 gatggtgaag caatgccgat tgaaaatcat ctgcaactca acgatgaaac cgaagagagt
    16021 cagccagata gcggagagga agaataatga ttgaacgcgg taaatttcgc tcactgacgc
    16081 tgattaactg gaacggcttt tttgcccgaa cttttgacct tgacgagctg gtcacgacgc
    16141 tttctggcgg taacggggcg ggtaaatcca ccaccatggc ggcgttcgtt acggcgctta
    16201 tccccgatct gaccctgctg catttccgta acactacgga agccggggcc accagcggtt
    16261 cgcgcgataa aggtctgcac ggtaagctga aagcgggtgt ctgttattcg atgctcgaca
    16321 ccattaactc gcgtcaccag cgcgtggtgg tcggtgtgcg tctgcaacag gttgccggac
    16381 gcgatcgtaa agtggatatc aagccgtttg ccattcaggg actgccgatg tcggtgcagc
    16441 cgacacaact ggtgaccgaa actctgaacg aacgccaggc gcgcgtgctg ccgcttaacg
    16501 agctgaaaga caagctcgag gcgatggaag gcgtgcagtt taaacagttc aactccatta
    16561 ccgattacca ctcgctgatg ttcgatctgg gcatcatcgc gcgtcgtctg cgctcagcgt
    16621 ctgaccgtag taaattctat cgtctgatcg aagcctcgct gtatggcggg atctccagcg
    16681 ccattactcg ttctctgcgc gactacctgt tgccggaaaa cagtggggtg cggaaagcgt
    16741 tccaggacat ggaagcggcg ctgcgtgaaa accgcatgac gctggaagcg attcgtgtca
    16801 cccagtcgga ccgtgacctg tttaagcatc tgatcagcga agccaccaac tacgtggcgg
    16861 cggactacat gcgtcacgcc aacgagcgcc gtgttcatct cgataaggcc ctggagtttc
    16921 gtcgcgagct acatacttcg cgtcagcaac tggcggctga gcagtacaaa cacgtcgaca
    16981 tggcgcgaga gctggctgag cacaacggtg ccgaaggcga tctggaagcg gattatcagg
    17041 cggccagcga tcacctgaac ctggtgcaaa ccgcactgcg tcagcaggag aagatcgaac
    17101 gctacgaagc ggatctcgat gagctgcaga tccgtctgga agagcaaaac gaagtggtgg
    17161 cagaagccat cgaacgccag gaagagaacg aggctcgtgc ggaagctgcc gaactggaag
    17221 tggacgagct gaaaagccag cttgctgact accagcaggc gctggacgtc cagcaaacgc
    17281 gcgcgatcca gtataaccag gcgattgctg cgcttaatcg tgccaaagaa ctgtgccatc
    17341 tgccggactt aaccgccgac agcgctgccg aatggctgga aaccttccag gcgaaagagc
    17401 tggaagcgac cgaaaaaatg ctctctcttg agcagaaaat gagcatggcg caaaccgcgc
    17461 acagccagtt tgagcaggct tatcagctgg tggtggcaat caacggcccg ctggcgcgta
    17521 acgaggcgtg ggatgtcgct cgcgaactat tgcgcgaagg ggtcgatcag cgtcacctgg
    17581 cagagcaggt tcagccgttg cggatgcgct taagcgaact ggaacagcgt ctgcgtgagc
    17641 agcaagaagc tgagcgtctg ctggcagatt tctgcaaacg tcagggcaag aattttgata
    17701 ttgatgaact ggaagctctg catcaggagc tggaagcacg cattgcctct ctttccgata
    17761 gcgtctctaa cgcccgagaa gagcggatgg cactgcgcca ggagcaggaa cagctgcagt
    17821 ctcgcattca gagtttgatg cagcgtgcgc cggtttggct ggcagcgcaa aacagtctca
    17881 accagttgag cgaacagtgc ggcgaagagt tcacctccag ccaggatgtc accgaatttc
    17941 tgcaacagtt gctggagcgt gagcgcgagg caattgttga acgcgatgaa gtaggcgcgc
    18001 gcaaaaacgc cgtcgatgaa gagatcgaac gtttaagcca gccaggtggt tctgaagatc
    18061 agcgtctgaa cgcgctggcg gagcgttttg gtggtgtgct gctgtcagaa atttatgacg
    18121 acgttagcct ggaagatgcg ccgtacttct cagcactgta tggcccgtca cgccacgcca
    18181 tcgtggtgcc agatctgtca caggtaaccg aacacctgga aggcttgacc gattgcccgg
    18241 aagatctcta tttgatcgaa ggggatccgc agtcattcga tgacagcgtg ttcagcgttg
    18301 atgagctgga aaaagcggtg gtggtgaaaa tcgccgatcg tcagtggcgt tattcacgtt
    18361 tcccggaagt gccgctgttt ggtcgtgctg cgcgtgaaag ccgtattgaa agcctccatg
    18421 ccgagcggga agtgctttcc gaacgcttcg ccacgctttc ctttgatgta cagaaaaccc
    18481 agcgtctgca tcaggcgttc agccgcttta tcggcagtca tctggcggtg gcgtttgagt
    18541 ctgacccgga agcagaaatc cgtcaactga acagccgtcg cgtcgaactg gaacgggcgt
    18601 tgagtaatca tgaaaatgat aaccagcagc agcgtattca gtttgagcag gcgaaagagg
    18661 gcgttacggc gctgaaccgc attctgccgc gtctcaacct gttggctgat gacagcctgg
    18721 cggatcgcgt cgatgaaatc cgcgaacgtc tggatgaagc ccaggaagcc gcgcgttttg
    18781 ttcagcagtt tggcaatcaa ctggcgaaac tggagccgat cgtttcggta ttgcagagcg
    18841 acccggaaca gttcgaacaa ttaaaagaag attacgcgta ctctcagcag atgcagcgcg
    18901 atgcccgtca gcaggcgttt gccctgacgg aagtggtgca gcgtcgtgcg cactttagct
    18961 actctgactc ggcagaaatg cttagcggta acagcgatct caacgaaaaa ctgcgtgaac
    19021 gtctggaaca ggcggaagcg gagcgtaccc gcgctcgtga agcgttgcgc ggacacgcag
    19081 cgcagttgaa tcagtacaac caggtgctgg cttcgctgaa aagttcgtat gacaccaaaa
    19141 aagagctact taacgatctg caacgtgaat tgcaggatat cggcgtgcgt gctgatagcg
    19201 gggcagaaga gcgggcacgt attcgccgtg acgagttgca cgcgcaactg agcaataacc
    19261 gttcacgacg caatcaactg gaaaaagcgc ttaccttctg cgaagcggag atggacaacc
    19321 tgacccgtaa actgcgcaag ctggagcggg attactttga gatgcgcgag caggtagtga
    19381 ccgccaaagc gggctggtgt gcggtgatgc gcatggtgaa agataatggc gttgagcgtc
    19441 gcttacaccg tcgtgagctg gcttatctct ccgctgatga tttgcgctcc atgtcggata
    19501 aggcgttagg tgcgctgcgt ctggcggtgg cggataacga acatctgcgc gacgtgctgc
    19561 ggatgtcgga agatccaaaa cgtccggaac gtaaaattca gttcttcgtg gcggtttatc
    19621 agcatctgcg tgaacgtatt cgtcaggata ttattcgtac cgatgatccg gtggaagcta
    19681 tcgaacagat ggagatcgag cttagccgtc tgaccgaaga attaacctct cgtgaacaga
    19741 aactggcgat cagttcccgc agcgtggcga acatcattcg caaaaccatt cagcgcgagc
    19801 agaaccgtat ccgtatgctc aaccaggggt tgcagaacgt atcgtttggc caggtgaaca
    19861 gcgtacgtct gaacgtgaac gtacgtgaaa cgcacgccat gctgctggat gtgctctctg
    19921 aacagcacga gcagcatcag gatctgttta acagcaaccg cctgaccttc tcagaagcgc
    19981 tggcgaaact gtatcaacgt cttaacccgc agattgatat ggggcagcgc acgccgcaga
    20041 ccatcggtga agaactgctg gattaccgca actatctgga aatggaagtt gaggttaacc
    20101 gtggttccga tggctggttg cgtgcagagt ctggtgcatt gtcgaccggt gaggcgattg
    20161 gtaccggtat gtcgattctg gtgatggtgg tacaaagctg ggaagatgaa tctcgccgcc
    20221 tgcgcggtaa agatatctct ccttgccgcc tgctgttcct cgatgaagca gcgcgactgg
    20281 acgcccgttc catcgccacg ctgtttgaat tgtgtgaacg tttgcaaatg cagctcatta
    20341 tcgcagcgcc ggaaaatatc agcccggaga aaggcaccac ctataaactg gtacgtaaag
    20401 tcttccagaa taccgaacac gttcacgtcg tcggcctgcg aggatttgcg cctcaactcc
    20461 ctgaaacgct tccaggaagt gacgaagcgc cttctcaggc gagttaaaat taaggcggca
    20521 gcaatgccgc cttttctttt tccgaaaact ccgtttctgc actaaaaaag tggcacatta
    20581 cggcgcgctt ttctttaaac ttctttacat taggttatgt aaaaacgtat cggcgtttat
    20641 atactgaaga taagcctgat gagtaacagg cttgctcgtc atactttcgt gagtattggc
    20701 gttgtacagg caagtcgtaa aataacagcc tggctattca gagtatgata aaaacagggg
    20761 gcaagggatg ttgcttaata tgatgtgtgg tcgtcggctg tcggcaatca gtttgtgcct
    20821 ggccgtaaca ttcgctccac tgttcaatgc gcaggccgat gagcctgaag taatccctgg
    20881 cgacagcccg gtggctgtca gtgaacaggg cgaggcactg ccgcaggcgc aagccacggc
    20941 aataatggcg gggatccagc cattgcctga aggtgcggct gaaaaagccc gcacgcaaat
    21001 cgaatctcaa ttacccgcag gttataagcc ggtttatctt aaccagcttc aattgttgta
    21061 tgccgcacgc gatatgcaac ccatgtggga aaaccgtgat gctgttaaag ccttccagca
    21121 acagctggca gaggtggcga ttgccggttt ccagccgcag tttaataaat gggtagagtt
    21181 actgaccgat cctggtgtta acgggatggc acgcgacgtg gtgctctctg atgcgatgat
    21241 gggctatctc catttcattg caaatattcc ggtcaaaggc actcgctggc tatatagcag
    21301 taaaccttat gcgcttgcaa cgccgccgct ttcggtgatt aaccaatggc agctggcgct
    21361 ggataaaggt caattgccga cgtttgttgc aggactggca ccgcagcatc cgcaatatgc
    21421 ggcgatgcat gaatcgttac tggccttact cagtgacacc aaaccgtggc ctcaactgac
    21481 cggtaaagca acgttgcgcc caggacagtg gagtaacgac gtaccggcgt tgcgcgaaat
    21541 attgcaacgt acaggcatgt tggacggggg accgaaaatt acgctacctg gcgatgacac
    21601 gccaactgac gcggtagtca gcccatcggc tgttactgtt gaaacagcag aaactaagct
    21661 gatggacaaa caaactacgt ctcgtagtaa acctgcgcct gccgttcgcg ccgcctacga
    21721 taatgaactg gtggaagccg ttaaacgttt tcaggcatgg caaggattgg gagcagatgg
    21781 tgctattggc ccggcaacgc gtgactggtt aaacgtaacg cccgcccagc gtgctggcgt
    21841 gttggcactc aacatccagc gattgcgctt gctgccaaca gagctttcta ccgggatcat
    21901 ggttaacatt ccagcctatt cgctggtcta ctatcagaac ggcaatcagg tgctggattc
    21961 gcgagtcatt gtcggtcgcc ccgatcgcaa aacgccgatg atgagcagtg cacttaacaa
    22021 cgtagtggta aacccgccgt ggaacgtacc accaactctg gcacgcaaag atattctgcc
    22081 gaaagtgcgc aacgatccgg gatatctcga aagccatggc tatacggtga tgcgcggctg
    22141 gaacagcaga gaagcgattg acccatggca ggttgactgg tctacaatca cggcctcgaa
    22201 tttaccgttc cgtttccagc aggctccagg cccacggaac tcgctggggc gctataaatt
    22261 caatatgccg agttcagagg ccatttattt gcatgacacg ccgaaccaca atctgttcaa
    22321 acgtgataca cgcgcattga gctcaggctg tgtacgagtg aataaagctt ccgatctggc
    22381 gaatatgctg ttgcaggatg caggatggaa tgacaaacgt atttctgatg cgctgaagca
    22441 gggcgatacg cgttacgtca atattcggca gtcgattccg gtgaatctct actatctgac
    22501 ggcctttgtt ggtgctgatg gtcgtaccca gtatcgtaca gatatttaca attatgatct
    22561 gcctgcgcga tccagctcgc aaattgtatc gaaagcggaa caattaatca ggtaaatgaa
    22621 gcagttcggg gaaatcagtt gtcgtaataa ttagcatgat cgggggcgat tctctgcagc
    22681 ccccgtcact gctggggttg agtcatcttg acgtcttctt tacgggcggt taaggtgcct
    22741 cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga ttatcatgga
    22801 caaattcgac gctaatcgcc gcaaactgct ggcgcttggt ggcgttgcac tcggtgccgc
    22861 catcctgccg accccggcgt ttgcaacact ctctacccca cgcccgcgca ttttgacact
    22921 caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca gaggctatat
    22981 tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga acaaaataaa
    23041 gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt taggcacccg
    23101 caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg aactacgcgc
    23161 ccgcagccgt ggagtagcga agaaaagcta tcacaccaaa ggccaggcga tggatttcca
    23221 cattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc gcgcaggtgg
    23281 tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag cacggcactg
    23341 gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc ggtcaccgca
    23401 ttctcccaga actgttcatt gatctggtgt gaacaaaccc gtctggccgc actggtcgat
    23461 cctggcggcg atgcggaaaa aatcaaacag gaagttgatg ccagcggcct gacactgatg
    23521 cagatcctgc tgactcatgg tcatctggac cacgttggcg cagcggcgga actggcgcaa
    23581 cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct gcaaggcttg
    23641 cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagc cgctgacgcc agatcgttgg
    23701 ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt acattgcccc
    23761 gggcatacgc cgggtcatgt ggtgtttttt aatgagcagg caaggctgct catttctggc
    23821 gatgttattt tcaaaggcgg agtagggcgc agtgatttcc cgcgtggcga tcacaatcaa
    23881 ctgatttctt caatcaaaga taaattgctg ccactggggg atgacgtgac atttattccg
    23941 ggtcacggac cattatccac tctcggttat gagcgcctgc ataatccctt cttgcaagac
    24001 gaaatgcctg tctggtaaag gcgcataaaa aagcccgctt tcaagcgggc ttcatcggtt
    24061 ttaacgctta cagcactgcc acaatcgctt cgcacagcgg tgccatgtta tctggtgtca
    24121 tcccggccac gtttacgcga ccagaagcaa cagcatatac gccaaactct tcgcgcagac
    24181 gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt ttgatgataa
    24241 agctgaagtc gcgatttgcg cctttttcct gcagcgtatt gacgaacaac tgacgcatac
    24301 gctgaatacg ctggcgcata tcagtcagtt cttgttccca aatcgcacgt aacgcatcgt
    24361 tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag tagttagcac
    24421 gaatcgccgc tttcatttgg ctgaatgcac gttcaacagt ttcactgtca gcagcaacca
    24481 gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag gaactggcaa
    24541 caatcagctc cttatgcaga gccgcgaaag cgcgcagtcc ttcagcatct tcttccagac
    24601 cccgagcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc tcaacggaga
    24661 gttgtgccag tgtctgccat tgttccagcg tagggtcgat gccggttggg ttatggcagc
    24721 agccatggaa cagcactacg tcgccagcct gggcttcgtt caggctgtta atcagtgcat
    24781 cgaagtcaag agtgtgattt tccgcatcat aataagcgta ttcacgaact tccagacctg
    24841 cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac acacgcttaa
    24901 cgctggtatt ttttgccagg aaatcggcag ccacgcgtag tgcgccagtg ccacccggag
    24961 tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca aacagcagtt
    25021 cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt ttggtggttt
    25081 cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg gttttacccg
    25141 tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca tcggcacgaa
    25201 acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc tcaaacatga
    25261 cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg agatttacaa
    25321 aattccaact attactgatg aaaacgcagg ctgtttttgc aagacgtgag attgctctgg
    25381 aaggcataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa agaggtgtgc
    25441 tattagaact ggtaaacgat acccacagca acggtatcgt cggaacctac gcccaacttg
    25501 ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt tttgttgaag
    25561 tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat accgtctacg
    25621 tctttcgctt tagatttagt gtaagcgatg gacggacgca gaccgaaatc gaactggtat
    25681 tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt tgtaaatttg
    25741 ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta gatgttgttc
    25801 gcgtcatact tcagaccagt agcccactgt tcagcttttt tgcctttgcc aaggggagac
    25861 tcttcctgca ggttggtacg gtcagcggca ccataagcac caacgatacc aaagccttcg
    25921 tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc agtgtcacgc
    25981 tcgtttttac ccaggtactg aacagcaaag ttcagaccat caaccagacc aaagaagttg
    26041 gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc gctgtatgca
    26101 gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata aaccacaccg
    26161 tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc gaatgccaga
    26221 cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg gaagttatat
    26281 tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt aaaaccaaga
    26341 cgggcatagg tcatgtcgcc attgccaccg taactgtctt caccgttacc cttggagaaa
    26401 taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt gttatagatt
    26461 tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc cagaatattg
    26521 cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca ctgccgtcaa
    26581 taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc tttatgtgtc
    26641 tgcaggcatc tttccattca aactaacgtt tcgctaccgt gaaagtgcta caaagataaa
    26701 gatttggttt caaaaagaaa aaatgtgtga caaaaagtaa aaattaagga actttgtgaa
    26761 caccgtcata tttccataga ggcgtgatga tatttacagc aatttttatc tatttatatg
    26821 atttccttat atttaaatta actaaacgga aattttgttt ctgatggaaa ctttatcgac
    26881 cgggcacaaa atcttctttc agacatccag aatgccaaaa aatcgtataa aaattgtgct
    26941 atcagctaag aaaaaactaa tccgcaataa gaatgcagat tattttttgt gcgtaaatgt
    27001 tgcagtgtaa tattccgtaa caggatgatc gttatggcag ggttgcaaat agattgcttg
    27061 acttaacaaa cagataactt gacagaaagg ataaaaaaag agccagcggg cgctggctct
    27121 tgaagacact aaattagaag ctggcgttac gcggagtacg tgggaacgga ataacatcac
    27181 gtacgttttg cacgccagtt acgtaagcaa tcaggcgttc aaaaccaaga ccgaaacctg
    27241 aatgcggaac agtaccgtag cgacgcagat cgcgatacca ccagtaatct tctttgttca
    27301 ggcccatttc aagcatacgc tcgtccagca cgtccagacg ttcttcacgc tgggagccac
    27361 caatgatctc accgatgccc ggagccagaa cgtccatagc cgcaacggtt ttaccgtctt
    27421 cgttaaggcg catatagaac gctttaatat ctttcggata gtttttaacc actactggtg
    27481 ctttaaagtg ttcttccgcc agataacgct catgctcaga agagagatcg acgccccagt
    27541 aaaccgggtt ttcaaacttc ctgccgcagt tttcgagaat ggtcactgcg tcggtgtaat
    27601 ccacctgcgc gaaatcggcc tcaatgaagc gttccagacg tgaaacggca tctttatcta
    27661 cgcgttcagc gaagaatttc atgtcgtcag cgcgttcttc gagaaccgct ttgaagacat
    27721 atttcagcat ggcttcagcc agacctgcga tatcgttcag gttagcaaac gccacttccg
    27781 gctccagcat ccagaattcc gccaggtgac ggctggtgtt ggagttttca gcacggaaag
    27841 tcgggccgaa ggtataaatt ttggacaatg cgcaagcgta ggtttcgccg ttcaactggc
    27901 cagatacggt caggaaagac tctttaccaa agaagtcttt gtcgaaatcc actttgccct
    27961 gatcgttacg cggcaggttt tccagatcca gcgtagaaac acggaacatt tcgcctgcac
    28021 cttcggtatc agatgcggta atcagtggcg ttgaaaccca gaagaatccc tgctcgttaa
    28081 agaagcgatg cagcgcctgc gccagcgtat ggcgaacgcg cgcgacggca ccaatcaggt
    28141 ttgtgcgcgg acgcaggtga gcgacttcac gcagatactc aatgctgtgg cgttttgccg
    28201 ccatcgggta agtgtttgga tcttcaaccc aaccagcaac ttcaaccttg ctggcctgaa
    28261 tttcaaattg ttgcccctgg cccggcgacg ccacgacttt acccgttaca atgaccgagc
    28321 agccggtggt cagacgcagg acgtcttcat tgtaattggg cagagaatta ttgatgacag
    28381 cctgtacagg atcaaagcag gaaccgtcat aaacggcgag gaaggagatg ccagcttttg
    28441 aatctcggcg ggtacgtacc catccgcgca cggtgacttc gctgtcaacg gctacgcggc
    28501 cctggagtac gtcggctaca ggcacaacgc tcataatatt ctctctgtta atagtcggaa
    28561 aaaataaaca cttgtccacc cgtaaatggg gggataccta tgttacctgg catctgcaat
    28621 cagacaagca gaattcgcaa atgcagcgaa agatttcgga attaaaggta aaaaaaggga
    28681 gccgattagc tccctgatga tattaactgg cttttttaat atgcggaagg tcgaacgctt
    28741 tgcgcagcgc ccgaacaaac gctttatcgt ggcagatcgt tttgccaggg ctgtcagaaa
    28801 gtttcgccac cggtttaccg ttacactcta ccaacttaat gacaatattc agaggtttta
    28861 cctgggggat atcgcaggtc aggcgagtcc caataccaaa acttaattgc acgcgggaag
    28921 agaagtggcg gtatagctca accgctttgc gtaaatccag attgtcagag aaaaccagcg
    28981 ttttactctg tggatcaatt cccagctttt cataatgtgc aatggctttt tcaccccatt
    29041 caagcgggtc gccagagtca tggcgcaggc cctggtaacg actggcaaat tccacgccga
    29101 aatctcgcag gaaagcatcc atggtgatgc agtcggttaa cgcaatgcca agttggtcgg
    29161 gatactcttc cagccaggca gcaagtgcag ctcgctggct gttggctaga tccgggctga
    29221 tttgctgatg cgcctggaac cattcgtgtg cctgtgttcc cattggcgtg agggaaagcc
    29281 gacgcgccag atcgtagttg ctggtgccca cgaaccagga ttcctgttgc agacgtttca
    29341 cgatggtttc ttgtacttcg cgagaaaaac ggcggcgggt gccaaaatcc atcagatgga
    29401 agcgcgacat atcaagaccg gcggttaatg ccgagaagtc gactaatttg ttttccagcg
    29461 tgtcgagggc ttgcgcaacg tcggcctgcg gtgagcgata gcgatgtacc atttcactga
    29521 taaccgccag caaaggaact tcccagagga tgacttcacg ccacgggccg cttaaacgaa
    29581 tatccagctt gccattatcg ttggacacgg tgacttgttc cgggttaaag cggaactcgc
    29641 gtagccagtt aagatagtcg gccttaaaga aaggcagggc agagagccac tgatattcat
    29701 catcctgcag gcgcaggtgc tgcatcgcct gaacctgttc acgaatagca tcggcataaa
    29761 tacccagcag atcgtcacct cggcaacgaa actccgccgc gacatgcaca tcgtaatagt
    29821 gatgaaacac ggcttgctgc atatgcaact tataagcatc tgtatccagc aacgagtgca
    29881 gaacaggaga agcgaattgt gtcataggtg cgcagtagcg tcctcgtaca ggagcgttta
    29941 gtacaataaa catcttcagg aactgctgga gtataccttg tttcgcaatt tattgaaccc
    30001 cgatcacacc atatgccacc tttctggtcg atggcataac gcggtgtatg ttataaaaat
    30061 gtagcaataa aggcgtttgt acctgaaaag atgaagattc tgcatagcgc gatttacgca
    30121 acaggaatag actgaacacc agactctata aaagatgcta aaggttattt atgactcaac
    30181 agccacaagc caaataccgt cacgattatc gtgcgccgga ttaccagatt actgatattg
    30241 acttgacctt tgacctggac gcgcaaaaga cggtcgttac cgcggtcagc caggctgtcc
    30301 gtcatggtgc atcagatgtt ccgcttcgtc tcaacggcga agacctcaaa ctggtttctg
    30361 ttcatattaa tgatgagccg tggaccgcct ggaaagaaga agagggcgca ctggtcatca
    30421 gtaatttgcc ggagcgtttt acgcttaaga tcgttaatga aattagcccg gcggcgaata
    30481 ctgcgctgga agggctttat cagtcaggcg atgcgctttg cacccagtgt gaagccgaag
    30541 gtttccgcca tattacgtat tatctcgacc gcccggacgt gctggcgcgt tttaccacca
    30601 aaattattgc cgataaaacc aaatatccct tcctgctttc caacggtaac cgcgttgcgc
    30661 aaggtgaact ggaaaatgga cgtcattggg tacagtggca ggatccattc ccgaaaccgt
    30721 gttacctgtt tgcgctggtg gctggtgatt ttgatgtact gcgcgatacc ttcactaccc
    30781 gctctggtcg tgaagtggcg ctggagttgt atgtcgatcg tggcaacctt gatcgtgcgc
    30841 cgtgggcaat gacctcgctg aaaaactcca tgaaatggga tgaagaacgc ttcggcctgg
    30901 agtatgacct cgacatctat atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg
    30961 agaataaggg gctgaatatc tttaactcca aatatgtgct ggcccgcacc gacaccgcca
    31021 ccgacaaaga ttacctcgat attgaacgcg ttatcggcca tgaatatttc cataactgga
    31081 ccggcaaccg tgtcacctgc cgcgactggt tccagctcag tctgaaagaa ggtttaaccg
    31141 tcttccgcga tcaggaattc agctctgacc ttggttcccg cgcggtaaac cgtatcaata
    31201 atgtgcgcac catgcgcgga ttgcagtttg cagaagacgc cagcccgatg gcgcacccaa
    31261 ttcgcccgga tatggtcatt gaaatgaaca acttctacac cctgaccgtt tacgagaagg
    31321 gcgcggaagt gattcgtatg atccacactc tgcttggcga agaaaatttc cagaaaggga
    31381 tgcagctcta tttcgagcgt catgatggta gcgcagcgac ctgtgacgat tttgtgcagg
    31441 cgatggaaga tgcgtcgaat gtcgatctct cccatttccg ccgttggtac agccagtccg
    31501 gcacgccggt tgtgaccgtc aaagacgact acaatccgga aactgagcag tacaccctga
    31561 ccatcagcca gcgcacgcca gctacgccgg atcaggcaga aaaacaaccg ctgcatattc
    31621 cgtttgccat cgaactgtat gacaacgaag gcaaagtgat cccgttgcag aaaggcggtc
    31681 atccggtgaa ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt tttgataatg
    31741 tctacttcca gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg aaactggaat
    31801 ataagtggag cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat gatttctctc
    31861 gctgggacgc ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc
    31921 agcaagggca gccgttgtct ctgccggtgc atgtggctga cgctttccgc gcggtgctgc
    31981 tcgatgagaa gattgatccg gcgctggcgg cagaaatcct gacgctgcct tccgtcaatg
    32041 aaatggcaga actgttcgat atcatcgatc cgattgctat tgccgaagta cgcgaagcac
    32101 tcactcgtac tctggcgact gaactggcag atgagctgct ggctatttac aacgcgaatt
    32161 accagagcga gtaccgtgtt gagcatgaag atattgcaaa acgcactctg cgtaatgcct
    32221 gcctgcgctt cctcgctttt ggtgaaacgc atctggctga tgtgctggtg agcaaacagt
    32281 tccacgaagc gaacaatatg accgatgcgc tggcggcgct ttctgcggcg gttgctgcac
    32341 aactgccatg ccgtgacgcg ctgatgcagg agtacgacga caagtggcat caggatggtc
    32401 tggtgatgga taaatggttt atcctgcaag ccaccagccc ggcggcgaat gtgctggaga
    32461 cggtacgtgg tctgttgcag catcgctcat ttaccatgag caacccgaac cgcattcgtt
    32521 cattgattgg cgcatttgcg ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg
    32581 gttatcagtt cctggtggaa atgcttaccg acctgaacag ccgaaatcca caagtggctt
    32641 cgcgcctgat tgaaccgttg attcgcctga aacgttacga tgccaaacgt caggagaaaa
    32701 tgcgcgcggc gctggaacag ttgaaagggc tggaaaatct ctctggcgat ctgtacgaga
    32761 agatcaccaa agcactggct tgataaataa ccgaatggcg gcaatagcgc cgccattcgg
    32821 agaatttacc cctgttttct caggcgagtt tcaaattcac cacgctgcat cacccgctgc
    32881 aatacttccg cttccagctc tgctaatcgt accgacccta accggcgcgg acgggggata
    32941 tccaccgtca aatccagacc aatttttccc tcttcaatta acaacacccg gtcagccatc
    33001 gccaccgctt cgctcacatc atgcgtcacc agcaagacgg taaagccgtg ctcctgccag
    33061 agtgatacaa tcaaatcctg catctcgagt cgcgttaaag cgtccagcgc accgagtggt
    33121 tcatcaagca acaataaacc tggtcgatga atcagcgccc ttgccagcgc cacacgttgt
    33181 ttctgcccgc cagaaagtgc ggcaggccat tcccccgcgc gattctccag tcctaccgca
    33241 gccagagccc gacgcgcggc atcgcgccac tggcctttaa ggcctaaccc aacgttatca
    33301 atcaccgatt tccacggcag cagacgcgcg tcctgaaaca tcattcgcgt atcatcctga
    33361 atctcagcca gcggtgtggt gcccgctaac acgtcgcctg cggttggcgt ttccagacct
    33421 gccagcaggc gcagcagggt acttttgcca ccaccgctgc ggcccaccac cgccacaaac
    33481 tgacctgctg gaatatgtaa atccagttgg ttcaggacga tattttccgc gtaatgtttg
    33541 cttactgcat tgagcaacaa tggtgtgccc tggttcagac gagcagtatt cataccgtgg
    33601 cctccttcaa atgataagcc gggttccagc gcaaccagag acgctctaac agctgcgcgc
    33661 tgacgtcagc aagtttgccg agcagggcgt aaagaataat ggcgactacc accacgtccg
    33721 tttgcagaaa ctcccgcgca ttcatcgcca gataaccaat gccggaattg gcagaaatag
    33781 tttcggcaac gatcagcgtc agccacatca ggcccaaagc aaaacgcaca ccgaccataa
    33841 ttgagggcag ggcaccaggc aggatcacat ggataaacag cggcatgccg gataatccat
    33901 agctacgtgc catctccacc agctcacggt cgatattacg gatgccatgc caggtgttga
    33961 tataaatggg gaatagcgta ccgagcgcca ccagaaagat ttttgcggat tcatcaatgc
    34021 caaaccacaa aatcaccagc gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa
    34081 ttgaggtatc cagcagtcgc tcaccccagc gtgacagccc gctaatcagc cccagaatta
    34141 atcccagcga tccgccaatt gaaaaaccaa tcagtgcacg ccaggagctg atcgccagat
    34201 gctgccacag ttcgccgctg gcagagagcg tccagaacgc catcaccact ccttccggtg
    34261 agggcaaaat acgcgtcgat agccagccaa ccgaggaggc cagttgccac accgccacga
    34321 tgcccaccgg taaaaaccag ggggcaacgc gcaataacca cttcttcact ggcgttgcca
    34381 tcatcgtgtt ccttagcttt gcgcgacgtt acgggggata aaatcattcg ccaccgcttc
    34441 gccttgtgga ttcagcggtt gtggctgggg aatttccggg atggcgacat ccagatgtgg
    34501 gaacagtaac tcgccgaccc gatacgcttc ttccagatgc gggtagcccg aaagcacaaa
    34561 actgtcgatg ccaagtgcag cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc
    34621 caccagcgct gtcccggcac cgccgcgcac taagccaaca cccgcccata aattggggct
    34681 gatctccaga ttgtcgcgct tgccgttatg tagcgccgcc attcgctgtt gtcctacgga
    34741 atccgttcgg gcgaatgagg cctgtgcttt ggcgatagtt tcatcatcaa gatgcgagat
    34801 taaccgctcg gcggcctgcc acgcttcgtc gttggtttca cgaacaatca catgcagacg
    34861 aataccgaaa cgaattttgc gtccatgcgc ggcagctttc gcccgcacgt gttcgatttt
    34921 ctcttttacc agtttcggtg gttcgcccca ggtaaggtag agatcaactt gttctgccgc
    34981 cagctcctgg gcgacatctg acgatccgcc aaagtaaagc ggaggataag gctgttgaat
    35041 cggcgggaag agcagtttgg ccccgcgtac atgaatatgt ttgccgttga aatccacagt
    35101 ctcgccaagc aacaaccgcc gccagacctg ggtaaattct gccgaggctt cgtaacgctc
    35161 gctatgatca aggaacacgc cgtcgcctgc cagttcttgg ggatcgctgc ctgtgaccag
    35221 gttaaacaac gcacgtccgt tagagagacg gtcaagcgtg gcggcctggc gggcggcaac
    35281 ggtaggtgag gttacgctgg ggcgcagggc gacaagaaac ttcagccgtt gtgtcaccgg
    35341 gatcatcgac gccgccacca gccacgcatc ttcacacgat cgccccgtcg ggatcagcac
    35401 accggtatag cccagacgat ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac
    35461 cggacgtgaa ccttcttccg ttcccagata atgcccgtca ccgtgggtcg gtaaaaacca
    35521 gaacatattc agactcataa ttgttttcct tccagttgag tgggctgcca gatgcgctgg
    35581 cgaatatcga cttttttcgg caccagacgg ttttcataaa acagatctgc cgtttgctgc
    35641 tgtaaggcgg caacttcagc gctcaacggt ttgatggtgg tgggaggacg atgatccaga
    35701 tacgaggcaa taaccggtgc cggtaagccc atcgtttttg ccagtaaagc gatgctttgt
    35761 tcgcgctggc tgcgggttaa cgcatcggcc tcactaaagg ttgtcagcac accctgaata
    35821 aaagcgccgt ttttttctgc atacggacga gctgccagat aaaacgatcc agtttgattg
    35881 agatcggtgc cgtctttcag cacccgcacg ccgccctgta ataatgcagc ggagtagtag
    35941 ggatcccaga tagcccaggc gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca
    36001 ggcgtcaggt aagtgggctg aatatccgtg aatttaagcc cggcctggcg aagggctcgc
    36061 agtaaaagat tgtgcgaact ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg
    36121 gctacggttt tgatcgggct gttttctgcc accagaatca cttcggcttt gggcttcggt
    36181 ggctcgacgc ccacgtatac caaatcagcc ccggcagcct gggcaaagat tggcggaata
    36241 tccccggtac tgccgagatc gatactgcca acgtttaacg cctccagcat ctgtgggcca
    36301 gcggggaact cgacccagga gatttttgtt tgcggatagc gtttttccag taactggtgg
    36361 ctttttgcca gcaccatacc aatactgcct ttttgatagc ctatacgtaa cgcttcaggc
    36421 gaggattctg cagcaaccgc aatcgtagag acgctaagta aacccgccag caccagttta
    36481 atgatcttac gcatgggcat tacctcgcaa agacagtagg tcaggaacct gaacatcgcg
    36541 gcggtgcaac gcctgccaga aagtttctag cgcggtatca agacgagttt gcagatttgg
    36601 cgtgaactgg ggtttgtgtt ggtaatcaat tacttgcgag tcatcggcaa acacgccgtg
    36661 caggatctcc tgggctttca gcgtacttaa aactggctta agggcataat cgaccgccag
    36721 cagatgggcc acggtgccgc ctgtcgccag cggcagcacc actttgcctt gcaacgcacg
    36781 ttctggcagc aggtcgagca gggttttcag tgcaccggaa taggcggctt tatacacagg
    36841 cgtggcgaca atcagcccat cggcctgttg cagctgttcg gtgaaggtct tgagtgccgg
    36901 actatcgaaa cgagcataaa gcagatcttc cgggacgaag ttttgcaaat tccagtgata
    36961 aacctctaca tccaggccat ttagtttttc ccgcgcatat tccagcaagg agctggagcg
    37021 agaaggaaat cgaggactac ccgccagggt gatgacacgc atactctctc cttataacca
    37081 attgttcttg ttttgttaac attgataaca attcggtcag tctgacagag ggcgtccgcg
    37141 agtcgaaatg atttatctga ggaagaaaag ccaaaaaaag cataaaaaaa gcgcaataag
    37201 aaaacgtttg ctatttattg ccgcaggtca attccctttt ggtccgaact cgcacataat
    37261 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
    37321 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
    37381 gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
    37441 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
    37501 gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc catcgagatc ggtaccgtca
    37561 cgccacgtcc ccagccaggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
    37621 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
    37681 aaaaagccca ttatgacggc gttctgggta ttaacatcgg caaaaataaa gatacgccgg
    37741 tggagcaggg taaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
    37801 atatcgccat caatatttca tcgccaaata ccccaggatt acgcacactg caatatggtg
    37861 aagcgctgga tgatctctta actgcgatta aaaataaaca aaatgatttg caagtgatgc
    37921 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gaagaattga
    37981 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
    38041 cactcgatcg ttctctggtt cagggaatga aaaattgtga tcaaaccggt ggcttaagtg
    38101 gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcacag gaattaaacg
    38161 gacgcttgcc gattattggt gttggcggca ttgactcggt tatcgctgcg cgtgaaaaga
    38221 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
    38281 tgattaaaga aatcgttacc catatctaat tatttcttca aattcctctt tataagataa
    38341 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
    38401 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt cgctgcggtt
    38461 ttataaggtt ggggagtggc agatgcgaat taaaccagac gataactggc gttggtatta
    38521 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
    38581 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggttttt gcgttgatga
    38641 cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca
    38701 aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac cgcaaatgcc
    38761 gaaaagctgg cattttgtat ctcatggtga aatgtggatg cccatgccgg gcgatgcggc
    38821 ctacgtttgg ttgagcgaca ctcacgagca agtgaattta ctggtcgttg aatctggcga
    38881 gaatgccgcg ctatgtctac tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
    38941 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
    39001 cttcagcctc gaacaggcag tctaacgcgc cagcttaagc gcagtcttcg gtacacaact
    39061 acagcaaaga atagtgccat catcgcctgt tgctgatttt tttagcggcg tgacttcgcc
    39121 ttctaaaagc tgaacacggc aacttccaca aatgcctgcg cggcaagaat aagggatacg
    39181 aattccctga ttttctaatt gctccagcag cacctgttgg ttatttccac gaaatgcctg
    39241 tccctgccag tcaatatcta cgtttgcgtc cggttgttgc gtgatgttgg cagtatcatc
    39301 agcggcacct gcgccgtaaa ttttagctgg agccgttgcc agaatttcca cctcatcgcc
    39361 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
    39421 atcctgggca gtacggaaag attgcagcgt ttttaatggt tcgtctgccg gatgtttttg
    39481 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggcttaa ccacatcaaa
    39541 cacgacatca ccaatgcgaa tcactttcca gctatcttct tcccaggccg atgcaccgga
    39601 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
    39661 agcacgtaac gaggcctcgt tagcaagaag gtaaggatag ccatcagcaa atgacagggg
    39721 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
    39781 ttcgcgggag aaaaatccac ttagccattt gttgatcgca tctggcgcaa ttcgcgcggt
    39841 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
    39901 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacgg gtgaaggagt
    39961 aaaccgtacc attagcggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
    40021 cataaagatg cgatcgaagg ccagaccact gacatctgcc agggtatgtg taagaccaat
    40081 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
    40141 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
    40201 acaattttct tagagtaaaa gtgacgatat gaattctctg tttgccagta cggcccgtgg
    40261 gctggaagag ctgttaaaaa ctgaactgga aaacctgggg gccgttgaat gccaggtggt
    40321 tcagggtggg gtccatttca agggcgacac acggcttgtt taccagagcc tgatgtggag
    40381 ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt aaggtttaca gtgatttaga
    40441 cctctatctc ggcgttcagg cgatcaactg gacagagatg tttaatcctg gcgcgacctt
    40501 cgctgtccat ttcagtggtt taaatgacac cattcgcaac agccagtacg gcgcgatgaa
    40561 agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat ctgccacgtc ccaatgttga
    40621 tcgcgatgcg ccggatattc gcgttaacgt ctggctgcat aaagaaaccg ccaatatcgc
    40681 ccttgatctc agtggtgatg gcttgcatct gcgtggctat cgtgatcgtg ctggtattgc
    40741 accgatcaaa gaaaccctgg cagccgcgat tgtgatgcga tccggctggc agccaggaac
    40801 accactgctc gatccaatgt gtggttctgg tacgttgctg attgaagcag cgatgctggc
    40861 gaccgaccgc gcaccgggct tgcaccgtgg ccgttggggc tttagcggct gggcgcagca
    40921 tgatgaagct atctggcagg aagtgaaagc ggaagcgcaa actcgcgccc gtaaaggcct
    40981 ggcagagtat agctctcatt tttacggttc ggacagcgac gcccgggtga ttcaacgtgc
    41041 gcgcactaac gcccgtcttg cggggattgg tgaactgatc acctttgagg tgaaagatgt
    41101 cgcgcaactg accaatccgc tgccgaaagg gccgtacggt acagtgttga gcaacccgcc
    41161 atacggtgaa cgtctggaca gcgaaccggc gctgattgcg ctgcatagtc tgctgggccg
    41221 aatcatgaaa aaccagttcg gtggctggaa tctctctttg tttagtgcct cgccggatct
    41281 gctaagctgc ctgcagctgc gtgcagacaa acagtacaag gcgaaaaacg gcccgctgga
    41341 ctgcgtacag aaaaactacc acgttgccga aagcacacca gacagcaaac cggcaatggt
    41401 agcggaagac tacgccaacc gcctgcgtaa gaacctcaaa aaattcgaga agtgggcacg
    41461 tcaggaaggg attgaatgtt atcgcctgta tgatgccgat ctgccggaat ataacgttgc
    41521 cgttgaccgt tatgccgact gggtggtggt gcaggagtat gcgccgccaa aaactattga
    41581 tgcgcacaaa gcgcgtcagc gtctgttcga tattatcgct gcaaccattt cggtactggg
    41641 gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgc cagaagggca aaaatcagta
    41701 ccagaaactg ggcgagaagg gcgagtttct tgaagttacc gaatataacg ctcacttgtg
    41761 ggtgaacttg acggattacc tcgataccgg tctgttcctc gatcaccgta tcgcccgtcg
    41821 tatgctcggt cagatgagca aaggcaaaga tttcctcaac ctgttctcct ataccggtag
    41881 cgccaccgtg catgcgggat taggcggcgc gcgcagcacc accaccgtgg atatgtcgcg
    41941 tacttatctg gagtgggcag aacgcaacct gcgtctgaat ggcctaaccg ggcgtgcgca
    42001 tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag gcaaatgaac agttcgatct
    42061 gatctttatc gatccgccaa ccttctctaa ctcaaaacga atggaagatg cgtttgatgt
    42121 tcagcgcgat catctggcgc tgatgaaaga tttgaaacgt ctgctgcgtg caggtgggac
    42181 gatcatgttc tcgaacaaca aacgcggctt ccgtatggat ctcgacggcc tggcgaaact
    42241 gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc caggatttcg cccgtaaccg
    42301 ccagatccac aactgctggc tgattaccgc agcctgaaag gaatagtaat gtcattaatc
    42361 agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc ttctcgataa cgcagaactg
    42421 catatcgaag ataacgaacg tgtttgtctg gtgggtcgta acggcgcagg caaatcgacg
    42481 ttaatgaaga tcctcaaccg tgaacagggg ctggatgacg gtcgcattat ttacgagcaa
    42541 gatttgattg tagctcgtct gcaacaggat ccgccgcgta acgttgaggg tagcgtttat
    42601 gatttcgttg ccgaaggcat tgaagaacaa gcggaatacc tgaaacgcta tcacgatatt
    42661 tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca acgaactggc gaaggttcag
    42721 gaacagctgg atcaccacaa cctgtggcag ctggaaaacc gcatcaacga agtgctggcg
    42781 caactgggat tagatcctaa tgttgcgctg tcgtcgcttt ccggtggctg gttgcgtaaa
    42841 gcggcattag gacgcgcgct ggtgagcaat ccgcgcgtat tgctgctcga cgaaccgact
    42901 aaccacctgg atattgaaac catcgactgg ctggaagggt tcctcaaaac cttcaatggg
    42961 acaattattt tcatttccca cgaccgttcg tttatccgca atatggcgac gcgcattgtt
    43021 gatctcgatc gcggcaagct ggtgacctat ccagggaatt acgaccagta cctgctggaa
    43081 aaagaagaag ccctgcgcgt ggaagaatta caaaatgccg agttcgatcg caaactggcg
    43141 caggaagagg tgtggatccg ccaggggatc aaagcacgtc gtacccgtaa tgaaggccgc
    43201 gtacgcgccc tgaaagcaat gcgtcgtgag cgtggtgaac gtcgcgaagt gatgggtacc
    43261 gcaaagatgc aggtggaaga agccagccgc tccggtaaga tcgttttcga aatggaggac
    43321 gtttgctacc aggttgacgg taagcaactg gtgaaagatt tttctgccca ggttctacgc
    43381 ggcgacaaaa ttgccctgat tggtccgaat gggtgcggca aaaccacgct gctgaaacta
    43441 atgctcggtc agcttcaagc agacagcggg cgtattcacg ttggcaccaa actggaagtg
    43501 gcttatttcg atcagcaccg cgcggaactg gatcccgata aaacggtgat ggataacctt
    43561 gccgaaggta agcaagaggt gatggttaac ggcaagccac gccacgtatt gggctatttg
    43621 caggacttcc tgttccatcc aaaacgggcg atgacgccgg tacgtgcgct ttctggcggt
    43681 gagcgtaacc gcttgttgtt ggcgcgtttg ttcctcaaac caagcaactt attgattctt
    43741 gacgaaccga ccaacgatct tgatgtcgaa acgctggaac tgctggaaga actgatcgac
    43801 agctatcagg gcacggtatt gctggttagc cacgatcgtc agtttgtcga taacaccgtt
    43861 acagaatgct ggatcttcga aggcggcggt aaaattggtc gttatgtcgg cggttatcat
    43921 gatgcccgtg gtcagcaaga acagtatgtg gcgctcaaac agcctgcggt gaaaaaaagc
    43981 gaagaagccg ccgcgccaaa agcagaaact gtaaaacgca gcagtagcaa actaagctat
    44041 aaattgcagc gtgaactgga gctgctaccg caattgcttg aagatctgga ggcgaagctg
    44101 gaagctttac agacgcaagt ggcggatgct tccttcttca gtcagccgca tgagcagacg
    44161 caaaaagtac tcgctgatat ggctgctgca gagcaggagc tggagcaagc ctttgaacgc
    44221 tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag
    44281 agactgctga caacggtaaa attgcctgat gtactgggtt tatcaggcat gaaaaccaac
    44341 actttgttag tcatctgcaa aatagcgcat cattaaggag taccaatgtg cgaacatcat
    44401 catgccgcga agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt
    44461 gagcatggtc agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat
    44521 gcccctcggc agcgtcccac cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc
    44581 aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta ccagtgaaat tacattactg
    44641 gaaattcccg gcgtgctttt ttctgaggac tacgccagcc tcggcacctt tttcctgttg
    44701 tttgtgcaac tggttcccgc gttttgtctg ataaccattc tgttactggt gaatcgcgcg
    44761 gaattaccgg tccgtttaaa agagcaactg gcacgggtgc tttttcaact caaaacctgg
    44821 ggaatggcgg agattttcct cgcgggtgtg ctggtcagtt tcgttaaact gatggcttac
    44881 ggcagcattg gggtaggcag cagctttctc ccctggtgtt tattttgtgt cctgcaactg
    44941 cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg acatcgcccc gatgccagaa
    45001 ctgcgccagc cgctaaaacc aggcgtcacg gggatacgcc aggggctgcg ttcgtgttcc
    45061 tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc cgcgttgtag taccaaaggg
    45121 tacgttcggc gtagaaacag cctgcaatgg acactcgcgc tgcttgtaac gtctatcatg
    45181 ctgtatcttc cggctaatat tttgcccatc atggtgacgg atttattagg ctcgaagatg
    45241 ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg aaggctctta tcccgtcgct
    45301 gcggtgattt ttctggccag tattatggtg ccaacgttaa agatgatcgc cattgcgtgg
    45361 ctgtgttggg atgccaaagg acacggcaag cgcgacagtg aaagaatgca tttgatttat
    45421 gaagttgttg agtttgtagg ccgctggtcg atgattgacg ttttcgttat cgcggtgctc
    45481 tcggcgctgg tgcgtatggg aggtttaatg agtatttatc cggcaatggg tgcattaatg
    45541 tttgctttag tcgtcataat gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg
    45601 tgggatcgtc aacctgaatc agagcatgag gagtcctgac agtatggaat ctaataatgg
    45661 ggaagccaaa atccagaaag tgaagaactg gtctcccgtg tggatatttc ctatcgtcac
    45721 ggcgctcatt ggggcctggg ttctttttta tcattacagc catcagggac cggaagtgac
    45781 cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa accaccatta aaagccgtag
    45841 cgttgacgtc ggcgtggttg aaagcgccac actggctgat gatttgacgc acgttgaaat
    45901 caaagcgcgg ctgaattccg gtatggaaaa attgctgcat aaagataccg tcttttgggt
    45961 ggtgaaaccg cagattggtc gagaagggat tagcggcctg ggaacgctgc tgtctggggt
    46021 ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg atttgctgga
    46081 ctcgccaccg ttggccccgc ctgatgcgaa aggtatccgt gtgcttctcg atagcaaaaa
    46141 agccgggcag ctctcgccag gggatccggt gctgttccgt ggctatcggg taggttcggt
    46201 tgaaaccagc actttcgata cgcaaaaacg caatatcagc tatcaactgt tcatcaatgc
    46261 accttatgac cgactggtga ccagcaatgt tcgtttctgg aaagatagtg gcattgcggt
    46321 tgatctgacg tcagcgggaa tgcgtgtgga gatgggctca ttgacaacgc tgcttagtgg
    46381 cggtgtcagc tttgatgtgc cggaaggtct ggatttaggg cagccagtgg caccgaaaac
    46441 agctttcgtt ttgtatgatg atcagaagag cattcaggat tcgttgtaca ccgatcacat
    46501 tgattatctg atgttcttta aagattcggt acgcggtctg caaccgggag ctccggtaga
    46561 attccggggt attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg
    46621 tcagacattt aacgatgatt accgtattcc ggtactgatt cgtatcgagc cagagcggct
    46681 gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac cttggcgaat tgttgaaacg
    46741 tggtttacgc ggatcgctga aaaccggaaa cctggtcact ggcgcactgt atgttgatct
    46801 cgatttctat ccaaatacgc ctgcaataac cggtattcgt gaatttaatg gttatcagat
    46861 tatcccgacc gtcagcggcg gcctggcgca aatccagcaa cgactgatgg aagcgttgga
    46921 taagatcaac aaactgccac tgaatccgat gattgaacag gcaaccagta cgctttctga
    46981 aagtcagcgc acaatgaaaa acctgcaaac gacgctggat agcatgaaca agatcctcgc
    47041 cagccagtcg atgcagcagt taccgacgga tatgcagtca acgttgcgtg aattgaatcg
    47101 cagtatgcag ggcttccagc ctggttccgc agcctacaac aagatggtgg cggatatgca
    47161 gcgccttgat caggtgttgc gagaactgca accggtgctg aaaacgctca acgagaagag
    47221 taacgcgctg gtatttgaag cgaaggacaa aaaagatcca gagccgaaga gggcgaaaca
    47281 atgaaaaagt ggctagtgac gattgcggca ctgtggctgg ccggatgcag ctccggtgaa
    47341 attaataaaa actattacca gttacctgtg gtgcagagcg gtacacaaag caccgccagc
    47401 cagggtaatc gcctgttatg ggttgagcag gtcgctgttc ctgactatct ggcggggaat
    47461 ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa cttgtgggcc
    47521 agcccgttgg atcaacagtt gcgcaacacc ctggttgcca acctgagtac gcaactgccc
    47581 ggctgggtgg ttgcctccca gcctctggga agcgcccagg acacgctcaa tgttaccgtc
    47641 acggagttta acggtcgcta tgatggcaag gtcattgtca gtggtgagtg gctgttgaac
    47701 caccagggac aactgatcaa acgtccgttc cgtctggaag gagtgcaaac tcaggatggt
    47761 tacgatgaga tggttaaagt gctggcccgt gtctggagtc aggaagccgc ttctattgcg
    47821 caagagataa agcgtctacc ttaatcataa agatttgtaa atataaccgt ctccggtatg
    47881 ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa gttgttcctc ttcacatttt
    47941 ttgtacaacc gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat
    48001 tgacggcagt tatgattcgc ggtattgctt aactgtggtt gcatatttag taatcactgt
    48061 tttcttttcc accagaaacc agtatgaggg aaacgaggca tgaagagaca aaaacgagat
    48121 cgcctggaac gggcacatca acgtggttat caggccggca tcgccggacg ctcaaaagaa
    48181 atgtgtccct atcagacgct gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc
    48241 atggcggaca gggtagtaat ggcctgattc tgtctcttta aaaagaaacc tccgcattgc
    48301 ggaggtttcg cctttttata ctctgtctga ttattatcag aaggcagacg tatcctggaa
    48361 cagaccgact ttcaggtcgt tggcggtata gatcaaacga ccatcaacca gcacttcgcc
    48421 atcagccagg cccataatca gacgacggtt aactatgcgt ttaaagtgaa tacgataggt
    48481 cacttttttc gccgtcggca gcacctgacc tgtgaatttc acttcaccta cgcccaacgc
    48541 gcgaccttta ccttcgccgc ccagccagcc gaggtagaac cctaccagct gccacattgc
    48601 gtccaggccc aggcatcccg gcataaccgg atcaccaatg aagtggcatc cgaagaacca
    48661 cagatccgga ttgatatcca gctctgcttc aacataccct ttgtcgaagt taccacccgt
    48721 ttcggtcatt ttgaccacac ggtccatcat cagcatgttt ggtgctggca attgcgggcc
    48781 tttagcgcca aacagttcac cgcgaccaga ggcaagaagg tcttcttttg tataggattc
    48841 gcgtttatct accatgttct ctgtaagcct tattttattg aagcacgcag gatagctaac
    48901 acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc agttcagcca acgtaatggc
    48961 catggaaaac ggtgacgtcc ttcctgttgc gatgcttgtg cgatacgttc ctggatggtt
    49021 tgcatcagcg tcgtctggcc ttcgccatcc cacaccagat ttaataataa cggcagtgcg
    49081 tcagtcacat cgtctactgc ccagatggtg aatttgcctt cttctaccgc tttcaccagt
    49141 tcactgtgaa gacttaaatg gcgaacgtta gcagtgggga taatgacacc ttgtttcccc
    49201 gttaactcac gttgctggca aatagcaaag aagccttcga ttttctcatt taaaccaccg
    49261 actggctggg cgcgaccgaa ctgatcgact gaacctgtga tagcgatact ctgattcacc
    49321 ggcacatcgg cgagggcgct gatcagagcg cagagttcag ccatcgaggc actatcgcca
    49381 tccacttcac tgtatgactg ctcaaatgtc agcgatgctg agaaggggat ctgttgctca
    49441 agctgtagtt ccgacatcag gaacgcttgc atgatcatca tccctttcgc atggatattg
    49501 ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac catcgccaat atgcacaacg
    49561 cagctaatgc gagaaggttc gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa
    49621 agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg ctcctgaagg
    49681 atctcatcct gcatgcgttc agcgaggaaa ccttcgcgcc attcacgctg ctgtagcatt
    49741 aagtttagct gctcgccgga gaaggtgtcg ccatcacaca gggaggcgac ctcttgacac
    49801 tggcggagga tccactgcgg gctaagcgga agtgtttctt gttcaccagt gtagcgtgct
    49861 gcttcgcgga taagtaccgg ccaggcatcc gctcccggtg ctggtaagtg attatgtctg
    49921 gcggtaaatg tcacccagcg acaccactgg cttactgact ccgcatcgac aatctgcaga
    49981 gtatcttcaa attcgctata aatagcctgc tctgaaagct ctggctccat ctcctggaaa
    50041 tcagccaatg attcgcgttc gcctaccaga atgactttca gcttcaatgg catcgaaggc
    50101 acagagacgg ggagagggcg cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta
    50161 acgatatttt tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg caaagagatg
    50221 atgagaatac cgccatttgc ctgatgcacc agaccaggct gcagggtaat atcgccatta
    50281 aactgacgca ggcagccaaa gagttgctct gcttctaccc agtcggcagc gacaacttgc
    50341 gttaaagtcg caaaattatc atctgcactc actgcgtgac gtaagcggat ggtgtggccg
    50401 gaaacctcat agtgaccgcc caccagttgg cctgcatcgc tttgtagcgt acgcgcggca
    50461 tcagcaataa gattcagata ctcagactct tccggggcct tcgccagcat aaaagaggag
    50521 gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca gtcgcggttg agtatcactg
    50581 aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg atagctatcg
    50641 gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag
    50701 tctgttgtca aagccgcgat tataccgtaa ccggcactgc agcacacgta gaaagcaacg
    50761 acaatacgcc tggcatgggc gttaaagctc acaggatgga gattcttttc ttcactggcc
    50821 taaaaagctg atattctgta aagagttaca cggtaacatt gagatcgcta tgaaatatca
    50881 acaacttgaa aatcttgaaa gcggttggaa atggaagtat ctggtgaaga agcaccgcga
    50941 aggggagtta atcacccgtt acatagaagc cagtgccgcc caggaagccg tcgatgagtt
    51001 gctctcgctg gaaaatgaac ccgtgctggt aaatggttgg attgacaagc atatgaatcc
    51061 ggaactggtc aatcgcatga agcagaccat tcgggccaga cggaagcgcc attttaatgc
    51121 ggaacaccag catacgcgca aaaaatcgat cgatctggaa tttatcgtct ggcaacgtct
    51181 ggctggcctt gcgcagcgtc gcggtaaaac gctttctgaa acgattgttc agctgattga
    51241 agatgcggaa aacaaagaga aatacgcgaa taaaatgtct tctttgaagc aggatctgca
    51301 ggcattgctg ggtaaggaat aactgtcaaa agtcagttca atttacgcaa ggcgaaaaaa
    51361 aaccccgcag cagcggggtt ttttaccaga cgagaactta agcctgcggc tgagttacaa
    51421 cgtctttgat gcctttaact tcgatctcta cgcgacgatc cggagccagg caatcgatca
    51481 gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg gattcgccca
    51541 taccacgtgc ggagattttg tctgccggga tacctttaga gatcaggtaa tcaacaacgg
    51601 actgagcacg acgctcggac agagcctggt tataagcgtc agaaccgatg cggtcagtgt
    51661 aacccagaac aactacggaa ccgtctttcg gatccaggtt gctcagctgg ctgtacagct
    51721 gatccagagc agcctgacct tccggtttca gggttgcttt gttgaaggtg aacagaacgt
    51781 cagacttcag agtgaagtgc ttggtctgta cttccggtgc cggagctgga gccggagcaa
    51841 ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac acccaggctc agcatgccgt
    51901 tgtccggacg agtgccgatg gtgtgtgcat caccgatgtt gttggtccac tggtattcca
    51961 gacgggtagc gatttcggga gtgatcgcat actcaacacc gccagcgaag accggagaaa
    52021 cgccggtgtc gtggtcttta aaggatgcgc caccaggtac gttagccttg gtgtctgcac
    52081 gccataccat accacccaga cgagtgtaga tgtccagatc gtcagtgatt gggtaaccca
    52141 gtttagcggt cagctgaacg ccctgagctt tgtatgcgcc gttgatgttg tcgcctttgt
    52201 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    52261 aaccaccaaa agcacctgca cccagttggt tttcgtgggt cggaccattg ttaggaataa
    52321 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    52381 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    52441 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    52501 ataacacggt taaatccttc accggggatc tgctcaatat taactctacc gatatcttcg
    52561 gcttatgccg agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg
    52621 tgaactccgt caggcatatg aaaaaaaagt cttgtataag gtacgtttaa tcttttttgt
    52681 cagcgacaat ttacagaaga aaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa
    52741 attagtcacg actgaaagca ttggctgggc gataaaaaaa gttccaggat taatcctaaa
    52801 tttacttaat gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgttgcgtga
    52861 gaggatgcgc ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca
    52921 acaagttcag catgctcttc tgcagtcaaa tcatctgcca accaaccgat caccacgctg
    52981 taattgcccg tgcgtaaagc gcgaaccatt gactccacgg tgtggcaagg ggagagctgg
    53041 ctaatctgca ttactttcgt taagggtagc ccagatgcct gaacccattc gcgactcagt
    53101 ttttgttgcg gtgttaacca gagttgccag cgtgattgct gaccgagttg ctgtaacaat
    53161 ggcaacagta gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata
    53221 agcccggctg tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac
    53281 gacgaagaac gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac
    53341 tgtatgaatg tacagtacat ccagtgacga caaagatcaa ccctattttc ggaaagagcc
    53401 tcgcaaattt tgtcgttggt ggcgggaaaa cataaattaa tcttgcccct taagaataag
    53461 ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt
    53521 taaaaggaat tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct
    53581 ggcaacgttg ggcacaattg aataccgatc attgtttggt agttacagcc agaccgttga
    53641 cgacacggtg tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag
    53701 tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc
    53761 cgttaccctc aattactatc gagttgatga aagtctatgg cgaaatcaac tgaagctggt
    53821 gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg
    53881 ggaaagactg aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt
    53941 ggggattaag gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact
    54001 gcggcagcaa aactgtctgg tgacagaaaa gattctgttt atgcttgaag gggccattat
    54061 cggcattcat gaagctgcgc tcccagtggc acgccgccag gagcttgcag aatgggctga
    54121 ctctcttacg ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga
    54181 ccaatctctg gcagtaatgc aatcaataac ccaacttgtt gtacgaccag aggttctttg
    54241 ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca ggccagctaa tgcctgattg
    54301 acgcgttctt catcagcagg ttgatgatgt agcgcgtcat caacatagca aacggcgtca
    54361 tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag
    54421 atatagctgg taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc
    54481 ggggtaacgt tcggttcgct ggacatattt gataccaccg acgccagttc agcatcacgg
    54541 ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat
    54601 tgttccagta tggcatcgag ataccggcag ttggcctctg tggcgcgttc gagcattcgc
    54661 ggcagattgc gaaacttcca gtcaggccag atgtagctca ctgccgccca cgcaatggca
    54721 caaccaatca gcgtatcgat tacgcgaggt aacgccactt caaaaccttc acccagcaag
    54781 ttgaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgggcgta ttgcacgtta
    54841 cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc ttccaatgat
    54901 ggcacaaacc acagcacagg aataccaatt gcgataccta ccagcgtacc aataatcctt
    54961 aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc
    55021 aagatccaat acccgtgatg cattccggtt atctgaatga tggcgtagcc gaagcacaac
    55081 accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta
    55141 agacgcagcc agatatcact caatccgtgc gggctgtcat cagcgagctc attttcgtca
    55201 ttattatggg gcagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg
    55261 gcacgtaaat tgttcagtaa aaatcccagt gttttgagta aatcagcagg cgcgccgtta
    55321 tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca
    55381 tgttgataag gctgacgcag caaaatacag cgtgacagtt gctggcacgc ctggccctgc
    55441 atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat
    55501 gtttgatatt gaatatgaga agagctggca cgctcgtgaa tatcctgcgc gacaaagtaa
    55561 taatgcagtg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc
    55621 gagagtttcg tctgattcaa tgtcgccatc agctgaccgt tggcgagagc caaatcgtac
    55681 agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga
    55741 taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac
    55801 agcagatgac caataagtgt caggacgttg taccagacgg caccggccag aagatacatc
    55861 ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc
    55921 aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg
    55981 ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact
    56041 gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc
    56101 agtcggtcat cgagatcggt cagcgccgct gccaccatcc caagcgtcag cggaatcgtc
    56161 agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata
    56221 cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata
    56281 aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcgcg cgcagcttgc
    56341 gctgtttcta ccgataccac gcgacgaccg accggccata gtgcaatagc ggcaatttta
    56401 aagttcgcaa taccgacagg aatgccaatg atggaaatac attgtgcgat gcccgttgca
    56461 atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta
    56521 ccgccagtat tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct
    56581 tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagtggt
    56641 aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca
    56701 aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact
    56761 gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct
    56821 gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc
    56881 gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc
    56941 cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc
    57001 gattcactgt aagcgcggtc tggtatgggg cgaactggag tttgtactgc cggacgaaaa
    57061 agtggtgcgt ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga
    57121 tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggcg ttttacgtca
    57181 gcaactggat ttgattgcca cgcgcaccgg agaaaataaa tggctgacgc gtgagcaaac
    57241 ctctggtatg cagcaacaaa tccgccaggc tttgtcggcg ttaccattgc cggttaaccg
    57301 actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga
    57361 cattgaaggc gctcggttgc agcataatca ggcgtatacc gaagccatgc ttaccgagta
    57421 tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt
    57481 cgttaatggc gagcattctt tgttagtgct ggctggtgca ggaagcggaa aaacgtcggt
    57541 gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt
    57601 attgctggcg tttggtcgca aagccgctga agagatggac gagcgtattc gagaacgact
    57661 acataccgaa gacattaccg cacgcacatt tcatgcgctg gcgctgcata ttattcagca
    57721 gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga
    57781 actttttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg
    57841 gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaatt tctgggatga
    57901 tgaaaaatta cagcgtcgtc ttgcctctcg cctcgatcgc tgggtaagtc tgatgcggat
    57961 gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt
    58021 cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc
    58081 agaaaacgct gtcgattttt cgggccttat tcaccaggcg attgtgattc tggagaaagg
    58141 tcgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca
    58201 gcgtgcagcg ttgttagcgg cattacgcaa gcaaaatagt caaacgacgt tgttcgctgt
    58261 tggtgatgac tggcaggcga tttaccgatt cagcggggcg caaatgtcgc tcaccacggc
    58321 tttccatgaa aactttggtg aaggtgatcg ctgtgattta gacacgacct accgttttaa
    58381 cagtcgtatc ggtgaggtgg caaacaggtt tattcagcag aacccaggcc agcttaaaaa
    58441 gccgctaaac agcttaacca atggagacaa aaaagccgtc acattattgg atgaggggca
    58501 acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagaac gcattctgat
    58561 cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc
    58621 gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt
    58681 catcatcgtt ggcttgcagg aggggagtga tggttttccg gctgcggcgc gggagtcgat
    58741 tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag aacggcggtt
    58801 aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa
    58861 tccctctcct tttgtcgaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta
    58921 agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc
    58981 tgataatcgg ggatcagaat atcgaccgcg tcgttaaaat gcggtgactg gataatgaag
    59041 tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgttgccag acgcagcaag
    59101 gctttcacgt caggatcgtg cggcacggca tttagtgggt cccagaagaa aatcaataca
    59161 tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc
    59221 aacatcgcgt tgacgttcat gccggtcgcg cgggaaatta agttaccggt agtgcctgtt
    59281 gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt
    59341 tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgaaggtaa agtgcgagtc
    59401 gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta agtacttatc
    59461 gctgcaagat aaagactgaa aaagcctgcg cacaggcaca aaaatctcag gaggatggtt
    59521 gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat
    59581 acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa
    59641 cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc
    59701 gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg
    59761 aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt
    59821 caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc
    59881 gaaaattgac ctggttgatg agctgggtat taaaactaac caccagtgga ggggagatat
    59941 agagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat
    60001 gcggtccatt gtccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct
    60061 taccgtcgag taccagaaga tcgacatcag tagaaagccg cagcgtggtg gcaaaaacgg
    60121 ataccggcaa acataatgca atcaaggtgg tcacgatgcc
//
LOCUS       DAJOTB010000010        59978 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJOTB010000010 DAJOTB010000000
VERSION     DAJOTB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.010"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..545)
                     /gene="malX"
                     /locus_tag="OHN28_000646"
     CDS             complement(<1..545)
                     /gene="malX"
                     /locus_tag="OHN28_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCQ2296092.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTR"
     gene            720..1748
                     /gene="malI"
                     /locus_tag="OHN28_000647"
     CDS             720..1748
                     /gene="malI"
                     /locus_tag="OHN28_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCQ2296093.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1860..2627
                     /gene="hdhA"
                     /locus_tag="OHN28_000648"
     CDS             1860..2627
                     /gene="hdhA"
                     /locus_tag="OHN28_000648"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCQ2296094.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            2856..3446
                     /gene="uidR"
                     /locus_tag="OHN28_000649"
     CDS             2856..3446
                     /gene="uidR"
                     /locus_tag="OHN28_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCQ2296095.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            3836..5647
                     /gene="uidA"
                     /locus_tag="OHN28_000650"
     CDS             3836..5647
                     /gene="uidA"
                     /locus_tag="OHN28_000650"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCQ2296096.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            5644..7017
                     /gene="uidB"
                     /locus_tag="OHN28_000651"
     CDS             5644..7017
                     /gene="uidB"
                     /locus_tag="OHN28_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCQ2296097.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVITGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            7056..8322
                     /gene="uidC"
                     /locus_tag="OHN28_000652"
                     /pseudo
     CDS             7056..8322
                     /gene="uidC"
                     /locus_tag="OHN28_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(8367..9875)
                     /locus_tag="OHN28_000653"
     CDS             complement(8367..9875)
                     /locus_tag="OHN28_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2296098.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(9976..11151)
                     /gene="manA"
                     /locus_tag="OHN28_000654"
     CDS             complement(9976..11151)
                     /gene="manA"
                     /locus_tag="OHN28_000654"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCQ2296099.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            11350..12996
                     /gene="fumB"
                     /locus_tag="OHN28_000655"
     CDS             11350..12996
                     /gene="fumB"
                     /locus_tag="OHN28_000655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2296100.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            13139..14542
                     /gene="fumC"
                     /locus_tag="OHN28_000656"
     CDS             13139..14542
                     /gene="fumC"
                     /locus_tag="OHN28_000656"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCQ2296101.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(14539..15468)
                     /gene="tus"
                     /locus_tag="OHN28_000657"
     CDS             complement(14539..15468)
                     /gene="tus"
                     /locus_tag="OHN28_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCQ2296102.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15544..16845)
                     /gene="rstB"
                     /locus_tag="OHN28_000658"
     CDS             complement(15544..16845)
                     /gene="rstB"
                     /locus_tag="OHN28_000658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCQ2296103.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(16849..17568)
                     /gene="rstA"
                     /locus_tag="OHN28_000659"
     CDS             complement(16849..17568)
                     /gene="rstA"
                     /locus_tag="OHN28_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCQ2296104.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            17697..18032
                     /locus_tag="OHN28_000660"
     CDS             17697..18032
                     /locus_tag="OHN28_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCQ2296105.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(18029..18751)
                     /gene="folM"
                     /locus_tag="OHN28_000661"
     CDS             complement(18029..18751)
                     /gene="folM"
                     /locus_tag="OHN28_000661"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCQ2296106.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(18788..20170)
                     /locus_tag="OHN28_000662"
     CDS             complement(18788..20170)
                     /locus_tag="OHN28_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2296107.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(20356..21300)
                     /locus_tag="OHN28_000663"
     CDS             complement(20356..21300)
                     /locus_tag="OHN28_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2296108.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21824..23356
                     /gene="pntA"
                     /locus_tag="OHN28_000664"
     CDS             21824..23356
                     /gene="pntA"
                     /locus_tag="OHN28_000664"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCQ2296109.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            23367..24755
                     /gene="pntB"
                     /locus_tag="OHN28_000665"
     CDS             23367..24755
                     /gene="pntB"
                     /locus_tag="OHN28_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCQ2296110.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(24780..25814)
                     /gene="tqsA"
                     /locus_tag="OHN28_000666"
     CDS             complement(24780..25814)
                     /gene="tqsA"
                     /locus_tag="OHN28_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCQ2296111.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            26226..26591
                     /gene="mdtJ"
                     /locus_tag="OHN28_000667"
     CDS             26226..26591
                     /gene="mdtJ"
                     /locus_tag="OHN28_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCQ2296112.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            26578..26907
                     /gene="mdtI"
                     /locus_tag="OHN28_000668"
     CDS             26578..26907
                     /gene="mdtI"
                     /locus_tag="OHN28_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCQ2296113.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(26946..27767)
                     /locus_tag="OHN28_000669"
     CDS             complement(26946..27767)
                     /locus_tag="OHN28_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCQ2296114.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(27867..27950)
                     /locus_tag="OHN28_000670"
     CDS             complement(27867..27950)
                     /locus_tag="OHN28_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296115.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(28043..28378)
                     /locus_tag="OHN28_000671"
     CDS             complement(28043..28378)
                     /locus_tag="OHN28_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCQ2296116.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(28775..30028)
                     /locus_tag="OHN28_000672"
     CDS             complement(28775..30028)
                     /locus_tag="OHN28_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296117.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            30135..31028
                     /locus_tag="OHN28_000673"
     CDS             30135..31028
                     /locus_tag="OHN28_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2296118.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            31163..32383
                     /gene="mlc"
                     /locus_tag="OHN28_000674"
                     /gene_synonym="dgsA"
     CDS             31163..32383
                     /gene="mlc"
                     /locus_tag="OHN28_000674"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCQ2296119.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            32508..33203
                     /gene="bioD"
                     /locus_tag="OHN28_000675"
     CDS             32508..33203
                     /gene="bioD"
                     /locus_tag="OHN28_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2296120.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(33156..34448)
                     /gene="clcB"
                     /locus_tag="OHN28_000676"
     CDS             complement(33156..34448)
                     /gene="clcB"
                     /locus_tag="OHN28_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCQ2296121.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLRLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(34609..35223)
                     /gene="dmsD"
                     /locus_tag="OHN28_000677"
     CDS             complement(34609..35223)
                     /gene="dmsD"
                     /locus_tag="OHN28_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCQ2296122.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(35266..36120)
                     /locus_tag="OHN28_000678"
     CDS             complement(35266..36120)
                     /locus_tag="OHN28_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCQ2296123.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(36122..36739)
                     /gene="dmsB"
                     /locus_tag="OHN28_000679"
     CDS             complement(36122..36739)
                     /gene="dmsB"
                     /locus_tag="OHN28_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2296124.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(36750..38319)
                     /locus_tag="OHN28_000680"
                     /pseudo
     CDS             complement(36750..38319)
                     /locus_tag="OHN28_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(38380..39489)
                     /locus_tag="OHN28_000681"
     CDS             complement(38380..39489)
                     /locus_tag="OHN28_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCQ2296125.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            39472..41052
                     /locus_tag="OHN28_000682"
     CDS             39472..41052
                     /locus_tag="OHN28_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2296126.1"
                     /translation="MTDINHDNTVYQSSSFSGSPYLYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTIPFLSVSTRSTDRRVATMRQRELAATAKSFMLDRPD
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPAVFTQE
                     RQPSVVFSSLVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLAVRD
                     SMLAAEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVEAEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLDNGL
                     VCIDINEDGDGKSVKNKHSVRLVPLTDGVCGFSLKSFLKWVDTQPADGPLFGMTPSAY
                     SSWFNSRVLTEALGDAENVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDLY
                     GKGHAVGRLADVLKTALL"
     gene            41469..41969
                     /locus_tag="OHN28_000683"
     CDS             41469..41969
                     /locus_tag="OHN28_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296127.1"
                     /translation="MAIKIQCYTESDNAAFFGDDSDLQHQLPAPTYDEPKARTGKPPV
                     VIDNRAVRAWLIETGCLSYTQLVEAYPWADDVFTGLSRATSGAVGAGLPVSKAYLFSA
                     LYGTNDIFSRNTAKSYADITGRSPSPRYLRHVNSVLQATAVNLAKVLKDRGDFTWWDY
                     NDQCLS"
     gene            42456..43214
                     /locus_tag="OHN28_000684"
     CDS             42456..43214
                     /locus_tag="OHN28_000684"
                     /inference="COORDINATES: protein motif:HMM:NF016283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCQ2296128.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPGQWRSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFI
                     ALRNDKVSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKL
                     VSAKALNAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQDN
                     HYNGQEGLKVLTKGYEWLKSNKVELVRLCAKGGL"
     gene            43483..43974
                     /locus_tag="OHN28_000685"
     CDS             43483..43974
                     /locus_tag="OHN28_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296129.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSRESMELLEALAEAGVAIGNFFSDDLMTAYGRRQQNKRAEAERIAKEQRIQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWL
                     DSL"
     gene            44056..45045
                     /locus_tag="OHN28_000686"
     CDS             44056..45045
                     /locus_tag="OHN28_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296130.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLTDRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(45923..47335)
                     /locus_tag="OHN28_000687"
                     /pseudo
     CDS             complement(45923..47335)
                     /locus_tag="OHN28_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(47534..47839)
                     /locus_tag="OHN28_000688"
     CDS             complement(47534..47839)
                     /locus_tag="OHN28_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCQ2296131.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            47947..48657
                     /locus_tag="OHN28_000689"
     CDS             47947..48657
                     /locus_tag="OHN28_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCQ2296132.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(48660..49220)
                     /gene="speG"
                     /locus_tag="OHN28_000690"
     CDS             complement(48660..49220)
                     /gene="speG"
                     /locus_tag="OHN28_000690"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCQ2296133.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(49255..49596)
                     /locus_tag="OHN28_000691"
     CDS             complement(49255..49596)
                     /locus_tag="OHN28_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCQ2296134.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            49731..50057
                     /locus_tag="OHN28_000692"
     CDS             49731..50057
                     /locus_tag="OHN28_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCQ2296135.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            50263..51477
                     /gene="rspA"
                     /locus_tag="OHN28_000693"
     CDS             50263..51477
                     /gene="rspA"
                     /locus_tag="OHN28_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCQ2296136.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            51489..52508
                     /locus_tag="OHN28_000694"
     CDS             51489..52508
                     /locus_tag="OHN28_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HCQ2296137.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            52566..53933
                     /gene="ydfJ"
                     /locus_tag="OHN28_000695"
     CDS             52566..53933
                     /gene="ydfJ"
                     /locus_tag="OHN28_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCQ2296138.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALQDAAEDQPHVVRAAQ
                     PSRSL"
     gene            54022..55482
                     /locus_tag="OHN28_000696"
     CDS             54022..55482
                     /locus_tag="OHN28_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2296139.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(55518..55721)
                     /gene="ydfZ"
                     /locus_tag="OHN28_000697"
     CDS             complement(55518..55721)
                     /gene="ydfZ"
                     /locus_tag="OHN28_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCQ2296140.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(55898..56584)
                     /gene="ydfH"
                     /locus_tag="OHN28_000698"
     CDS             complement(55898..56584)
                     /gene="ydfH"
                     /locus_tag="OHN28_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCQ2296141.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(56673..57419)
                     /gene="ydfG"
                     /locus_tag="OHN28_000699"
     CDS             complement(56673..57419)
                     /gene="ydfG"
                     /locus_tag="OHN28_000699"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCQ2296142.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            57556..59601
                     /gene="dcp"
                     /locus_tag="OHN28_000700"
     CDS             57556..59601
                     /gene="dcp"
                     /locus_tag="OHN28_000700"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCQ2296143.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            59750..59854
                     /locus_tag="OHN28_000701"
                     /pseudo
     CDS             59750..59854
                     /locus_tag="OHN28_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT        15015 a        14496 c        15444 g        15023 t
ORIGIN      
        1 aagcgcgtac cgccgaagaa tgccagcgca tccggcaggc ggatattatg gaatcgctca
       61 tgcagcatcc agacgataat accggcgatc accgcaccga ggatcccggt gtcgatcgac
      121 tggatcccaa gaatgctctg gatgttattg gctttcagaa cggcggcgtc catggttggc
      181 agaatgcctt tattggtcaa ccagaagttt accgcgaggt tcattaccgc ataaccgacg
      241 aagccagcga atgccgctac gcctttgttt tcgcgtgcca gacccagcgg gatggcgata
      301 cagaacatga ctggcaggaa actaaaagca aacgagccaa tcttactcat ccaggtaaag
      361 atagcctgca acacggggtt gcccaggacc gggatcaggg tgatgacatc atggctgcta
      421 agagaactac caatgccgag cataatgccg cagaacgaca ataatgccac gggtaacatg
      481 aaggttttgc ctaactgctg gaagaactcc cacagcgtga ctttcggtgc tgttttcgcc
      541 gtcataaaac gactcctcgt agagaacaat ctggattcag taaattgcgc gagaagataa
      601 aacgttttat caaattttag tgaggcataa atcacattac gcaacgataa tagcgggtat
      661 aagataaata aaaggtaaaa cgttttatct gtcacataat cagggagtag gtcatctgca
      721 tggctaccgc caaaaaaata accattcatg atgttgcgct ggctgcgggc gtgtcggtaa
      781 gtaccgtttc gctggtgctt agtggcaaag ggcgaatctc taccgccaca ggagaacgcg
      841 ttaacgccgc cattgaagag ctgggatttg tgcgcaatcg gcaggcgtcg gcgctgcgcg
      901 gcgggcaaag tggcgtcatt ggtttgatcg tccgtgattt atctgcgccg ttttacgccg
      961 aattgacggc cggattgacg gaagctctgg aagcgcaggg acggatggtt tttttgcttc
     1021 acggcggtaa agacggcgag cagctggcac agcggttttc actattactg aatcagggtg
     1081 tcgatggtgt ggtaattgcc ggggctgcag gaagcagcga tgacctgcga cggatagcag
     1141 aagaaaaagc tatcccggtg attttcgctt cccgtgccag ttatcttgat gacgttgata
     1201 cggttcgccc ggacaacatg caggctgcac agttgttgac ggagcatctc attcgcaatg
     1261 ggcatcagcg tatcgcctgg ctgggagggc aaagttcctc attaacccgg gcagaacggg
     1321 tggggggcta ttgtgcgact ctactaaaat ttggcctgcc gtttcacagc gattgggtgc
     1381 tggagtgcac ttccagccag aagcaagccg cggaagctat cacggcgctt ttacgtcata
     1441 acccgaccat cagcgccgtg gtttgctata acgaaactat tgcgatgggg gcatggtttg
     1501 gtttgctgaa agcaggaagg caaagcgggg aaagcggagt cgatcgttac tttgagcaac
     1561 aggtttcgct ggcggcattt accgatgcaa cgcctaccac acttgatgat atacccgtta
     1621 tctgggccag cacgccagcg cgggaacttg gtaccacact tgcggatcgc atgatgcaaa
     1681 aaatcaccca tgaagagacg cattcacgca atcttattat tcccgcccgg ctcattgcgg
     1741 cgaaataatc ctctctttat ctgctatacc tggtagtgtc ctttcctcaa ggttaatgat
     1801 aaaaaatgtg gcacacaggc ggtgcctttt atttttactg aagttcacag gaggtttatg
     1861 tgtttaattc tgacaacctg agactcgacg gaaaatgcgc catcattaca ggtgcgggtg
     1921 caggtattgg taaagaaatc gccattacat tcgcgacagc tggcgcatct gtggtggtca
     1981 gtgatattaa cgccgatgca gctaatcatg ttgtagatga aattcaacaa ctgggtggtc
     2041 aggcatttgc ctgccgctgc gatattactt ccgaacagga actctctgca ctggcagact
     2101 ttgccgtcag taagttgggt aaagttgata tcctggttaa caacgccggt ggcggtgggc
     2161 ctaaaccgtt tgatatgcca atggcagatt ttcgccgcgc ttatgaactg aatgtatttt
     2221 cttttttcca tctgtcacaa cttattgcgc cagaaatgga aaaaaatggc ggtggcgtta
     2281 ttctgaccat tacttctatg gcggcagaaa ataaaaatat aaacatgacc tcctatgcat
     2341 catctaaagc tgcggccagt catctggtca gaaatatggc gtttgacctg ggtgaaaaaa
     2401 atattcgggt gaatggcatt gcgccggggg caatattaac caatgccctg aaatccgtta
     2461 ttaccccaga aattgaacag aaaatgttgc aacacacacc aatcagacgt ctgggccaac
     2521 cgcaagatat tgctaacgcg gcgctgttcc tttgttcgcc tgcagccagc tgggtaagcg
     2581 gacaaattct caccgtctcc ggtggtgggg tacaggaact caattaatac actaacgaac
     2641 cggtaaacag ccgtatgtgt tgtttaccgg gatagcgtgt gcaactcgtc aacgtctctg
     2701 ctaaacaact ggcagccaaa tcacggctat tggttaacca atatctggat aaaaagctta
     2761 cgaattgagt gtgccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg
     2821 gtaaactagt tattcatgct aactataatg gtttaatgat ggataacatg cagactgaag
     2881 cacaaccgac acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat
     2941 ttcacagtgc ctcgatgaaa gccatctgta aatcctgcgc cattagtccc gggacgctct
     3001 atcaccattt catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga
     3061 gggcgctggc ccgtttccgg gaaccgattg aagggattca tttcgttgac tatatggtcg
     3121 agtctattgt ctctctcacc catgaagcct ttgggcaacg agcgctggtg gtcgaaatta
     3181 tggcggaagg gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga
     3241 tcacggaatt tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag
     3301 acatcaacac ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg
     3361 atatcgaagc ggaagatctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga
     3421 ttggcggtat attaaccgca tcctgatcct ctctctcttt ccgcgggctg gtgacaactg
     3481 agcccgcgtt tcatatcgta atttctctgt gcaaaaatca tcctcccagg gatcggagaa
     3541 ttccacctaa atattcatcg tagcaatata tcatgagggt ttatcgttcc caatatgttc
     3601 gaatgaacgt tcgattgctt attttgtggc ttctgtcaac gctgttttaa agattaatgc
     3661 gatcaatatc acgctgtggg tattgcagtt tttggttttt tgatcgcggt gtcagttctt
     3721 tttatttcca tttctcttcc atgggtttct cacagataac tgtgtgcaac acggaattgg
     3781 ttaactaatc agattaaagg ttgaccagta tttttatctt aatgaggagt cccttatgtt
     3841 acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag
     3901 tctggatcgc gaaaactgtg gaattgatca gcattggtgg gaaagcgcgt tacaagaaag
     3961 ccgggcaatt gctgtgccgg gcagttttaa cgatcagttc gccgatgcag atattcgtaa
     4021 ttatgtgggc aacgtctggt atcagcgcga agtttttata ccgaaaggtt gggcgggcca
     4081 gcgtattgta ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg taaataatca
     4141 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca caccgtatgt
     4201 tattgccggg aaaagtgtac gtatcactgt ttgtgtgaac aacgaactga actggcagac
     4261 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttactttca
     4321 tgatttcttt aactacgccg ggatccatcg cagcgtaatg ctctacacca cgccgaacac
     4381 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcatctgt
     4441 tgactggcag gtggtggcaa atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
     4501 ggtggttgca actggacaag gcaccagcgg gactttgcaa gtagtgaatc cgcacctctg
     4561 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
     4621 tgatatctac ctgctgcgcg tcggtatccg gtcagtggca gtgaagggcg aacagttcct
     4681 gatcaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggatttgcg
     4741 cggcaaagga ttcgataacg tgctgatggt gcacgatcac gcactaatgg actggattgg
     4801 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
     4861 tgaacatgga atcgtggtga ttgatgaaac tgcagctgtc ggctttaacc tctctttagg
     4921 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
     4981 ggaaacccag caggcgcact tacaggcgat taaagagctg attgcgcgtg acaaaaacca
     5041 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
     5101 ggaatatttt gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
     5161 ctgtgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
     5221 gctgtgcctg aaccgttatt acggttggta tgtccaaagc ggcgatttgg aaacggcaga
     5281 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
     5341 caccgaatac ggtgtggata cgttagccgg cctgcactca atgtacaccg acatgtggag
     5401 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
     5461 tgtcgtcggt gagcaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
     5521 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
     5581 tctgctgcaa aaacgctgga ccggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
     5641 acaatgaatc aacaactctc ctggcgcgcc atcgtcggct acagcctcgg tgacgtcgcc
     5701 aataacttcg ccttcgcaat gggggcgctc ttcctgttga gttactacac cgacgtcgct
     5761 ggcgtcggtg ccgctgcggc gggcaccatg ctgttactgg tgcgggtatt cgatgccttc
     5821 gccgatgtct ttgccggacg agtggtggac agtgtgaata cccgatgggg aaaattccgc
     5881 ccgtttttac tcttcggtac tgcgccgtta atgatcttca gcgtgctggt attctgggtg
     5941 ccgaccgact ggagccatag cagcaaagtt gtgtatgcat atttgaccta catgggactc
     6001 gggctttgct acagcctggt gaatattcct tatggttcac ttgctaccgc gatgacccaa
     6061 caaccacaat cccgcgcccg tctgggcgcg gctcgcggga ttgccgcttc attgaccttt
     6121 gtctgcctgg catttctgat aggaccgagc attaagaatt ccagcccgga agagatggtg
     6181 tcggtatatc atttctggac aattgtgctg gcgattgccg gaatggtgct ttacttcatc
     6241 tgcttcaaat cgacgcgtga gaatgtggta cgtatcgtgg cgcagccgtc attgaagatc
     6301 agtctgcaaa ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg tgcgctgtgt
     6361 gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt tctacgtgcg ctatgtgtta
     6421 aatgataccg ggctgttcac tgtgctggta ctggtgcaaa acctggtcgg tactgtggca
     6481 tcggcaccgc tggtgccggg gatggtcgcg aggatcggta aaaagaatac cttcctgatt
     6541 ggcgctttgc tgggaacctg cggttatctg ctgttcttct gggtttccgt ctggtcactg
     6601 ccggtggcgt tggttgcgtt ggccatcgca tcaattggcc agggcgttac catgaccgtg
     6661 atgtgggcac tggaagctga taccgtagaa tacggtgaat acctgaccgg cgtgcgaatt
     6721 gaagggctca cctattcact attctcattt acccgtaaat gcggtcaggc aatcggaggt
     6781 tcaattcctg cctttatttt gggattaagc ggatatatcg ccaatcaggc gcaaacgccg
     6841 gaagttatta cgggcatccg cacatcaatt gccttagtac cttgcggatt tatgctactg
     6901 gcattcgtca ttatctggtt ttatccgctc acggataaaa aattcaaaga aatcgttgtt
     6961 gaaattgata atcgtaaaaa agtgcagcag caattaatca acgatatcac tagttaatat
     7021 tcaataaaaa taatcagaac atcaaaggtg caactatgag aaaaatagtg gccatggccg
     7081 ttatttgcct gacggctgcc tctggcctta cctctgctta cgcggcacaa ctggctgacg
     7141 atgaagcggg actacgcatc agactgaaaa acgaattacg cagggctgat aagcccagtg
     7201 ctggcacggg aagagatatt tacgcatggg tacagggagg attgctcgat ttcaatagtg
     7261 gttattattc caataattat tggcgttgaa ggcggggcgt attatgttta taaattaggt
     7321 gctcgtgctg atatgagtac ccggtggtat cttgatggtg ataaaagttt tggctttgcc
     7381 ctgggggcag taaaaataaa acccagtgaa aatagcctgc taaaattagg tcgcttcggg
     7441 acggattata gttatggtag cttaccttat cgtattccgt taatggctgg cagttcgcaa
     7501 cgtacattac cgactgtttc tgaaggagca ttaggttatt gggctttaac gccaaatatt
     7561 gatctgtggg gaatgtggcg ttcacgagta tttttatgga ccgattcaac aaccggtatt
     7621 cgtgatgaag gtgtttataa cagccagacg ggaaaatacg ataaacatcg cgcacgttct
     7681 tttttagccg ccagttggca tgatgatacc agtcgctatt ctctgggggc atcggtacag
     7741 aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa gcataccgct tgacccgaat
     7801 tatacgttga aaggggagtt gctcggcttt tacgcgcaac ttgaaggttt aagtcgtaat
     7861 accagccagc ccaatgaaac ggcgttggtt agtggacaat tgacctggaa tgcgccgtgg
     7921 ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa tgaatggtgc cgtggtggat
     7981 accgacattg gctatccctt ttcattaagt cttgatcgta accgtgaagg aatgcagtcc
     8041 tggcaattgg gcgtcaacta tcgtttaact ccgcaattta cgctgacatt tgcaccgatt
     8101 gtgacccgcg gctatgaatc cagtaaacga gatgtgcgga ttgaaggagc gggtatctta
     8161 gggggtatga actatcgggt cagcgaaggg ccgttacaag ggatgaattt ctttcttgct
     8221 gccgataaag gacgggaaaa gcgcgatggc agtacgctgg gcgatcgcct gaattactgg
     8281 gatgtgaaaa tgagtattca gtatgacttt atgctgaagt aagaaaaaac gccggagaga
     8341 aaaatctccg gcgttttggc tgtgaattac tgctgcggaa tagcgggtac agccggaacg
     8401 tttaatgtcg gcatcgcaaa catacccacg aaatcttcca gtggcatttt ctgcccgttt
     8461 aacgttatct gaccgttagc atattgcagg ctggtggtga tggtattgtc ctgcaaggtg
     8521 gtcagacgga acatctgccc cattgctgat gcaccttcaa cttgctgttt cgccagtttt
     8581 ttcgcttgat cttcctgata accttccagc ttcgctacct gagtcatcaa ctcagttgcc
     8641 atatccaccg gaatggtcag tttcgcatcc agagatttaa ccgagcggtc cacttcctgc
     8701 gccagcgttt gcggcgcttc tttagtcgtt gccggatctt tcaggaacag cgacagattc
     8761 agcgcacttt caccctgact gtttttccag cttagcggcg cgatagtaat caccggatcg
     8821 cctttcagca tcagcggcag agcgctaaag aaggcttccg tcactttctc ctgataaagt
     8881 tcgggattgt tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc gttatattgc
     8941 tggctaaact gatgccaggc ttcgccatca atttggccga cttttaaagt cagcttgcca
     9001 ctgcccagat cctgattctg taccttcagg ctgtttagcg agtaatccag ttggctattg
     9061 atcgttttac cgtcattgac cagatccgat ttaccgctga tctccatgcc ttccagcagt
     9121 gccagttctt tgccctccac tgaaatggtc attttttcca gcgacagttt ttggtttcct
     9181 acgcgctcac caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtt
     9241 aactgcactt tctggttgta ttcgttaacc gcgtctatcc gaccactttg cgcctccccg
     9301 gaaagggaga tagctttgcc gtctctgtct gcatttaact ggaactcgcc gccgctaaag
     9361 gcgacttttt catccttttg ctcgtaattc agtggcttga gcgaaatgtc ggaactggaa
     9421 tctccgctgt aaccaatgcg ggagttaatc tcaaaaggcg tttcaccttt tgccatatca
     9481 aacagtggtt tgcttacttc gttattaacc agcgtggttt gaattgatgc catcgacggg
     9541 atcagattca gttttttaag ctgggcaagc gggaagggac catgatcaac cgattcgttg
     9601 aagatgacgc tctgaccgct tttaatccac ggatttactt ttccggcaat gggtttcacc
     9661 aacagttgca actggctgct gaatacgccg cgatgatagt tttgataact gacttccagg
     9721 ttggattcag gagctgtcag tttgagttgc gcgttcgcct gcgcgaccat gtcttcgaga
     9781 tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg tccagactac gcctagcgca
     9841 acaatgacgc ctaccgctac cagcgattta ttcataatga ttatccataa aatgaaatca
     9901 ggcggactgg ccgcctgaag gtgttataag cctttaataa gcttagcaag agatgttaat
     9961 tttttcagta agctcttaca gcttgttgta aacacgcgct aaacggccgt ggcctttgac
    10021 agtcaccggt gattcgttgg cggcaataaa cgctgattca cccggtttaa gctgtaactg
    10081 ctgagaacct ttacacagcg ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg
    10141 ctggctgatg gtggtttctt tatcactaag gtcatgcaac gagaaggcaa aatcatccac
    10201 aggaatcggg aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca acttgttagc
    10261 cggtttggct tcgaacttca cgttggcaac cagttccgga atatcaatgt atttaggcgt
    10321 cagacctgca cgcagcacgt tatcggagtt cgccatcact tccagcgcca cgccttgcag
    10381 gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg ccaggattca atttcaccac
    10441 attcagcaac agcggggaga acaggccgct gtcttccggg taaaattcag aaattaaacg
    10501 aatcgtttgc catggttcac cctgctggct gtcgagggcc gattttaaaa tcgccagcgc
    10561 gcgggatttt tcttcaccct gcatattcaa caggctggcg aacagttcgc ttaaacgttc
    10621 ggcatcaggc tgttgtaaaa agtgagcaat cgccggatgt gcacctgcga ccggctggag
    10681 cagggagaca atctcggaaa actcacgaaa cgcgttcatc gcgaggaaag gggtcagggc
    10741 gaaaaccagc tcaggcttgt gattaggatc tttatagtta cgctcagcgg catccatcgg
    10801 aatacctgca gcattttctt tggcaaaacc gatttcagaa ttgcgtttgt ttggatgaac
    10861 ctgaatggag agcggctggg ctgcgcataa tactttgaac aggaaaggca actcgccaaa
    10921 gcgtttggca acggcatctc ccaaaagagt cgctttatca ctctcaatca catcacgcag
    10981 tgaaacgata tctcctgcgg catcgtgcac acgtgaactg ctctttggat gcgcgcccat
    11041 ccacaactcg gccatcggct ggctggacgg attttccata ccataaagtt cagtcaacgc
    11101 cgttttgctg ccccaggcat agttttgcac tgagttaatg agtttttgca tgatcaatcc
    11161 ctgttttaat gtggaaatta atcccgctat taaagcaaga atcctacggg aagtaacctg
    11221 gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa aagtgtgtag gatattgtta
    11281 ctcgctttta acagggcaac ggaacacccg cccagagcat aaccaaacca ggcagtaagt
    11341 gagagaacaa tgtcaaacaa accctttcat tatcaggctc cttttccact caaaaaagat
    11401 gatactgagt attacctgct aaccagcgaa cacgttagcg tatctgaatt tgaagggcag
    11461 gagattttga aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat
    11521 gcgtcattta tgctgcgtcc ggctcaccaa caacaggtgg ccgacattct gtgtgacccg
    11581 gaggccagcg aaaatgataa atatgtggcg ctgcaattcc tgcgtaactc cgacatcgcg
    11641 gcaaaaggcg ttctgccaac ctgtcaggat accggcaccg cgattattgt tggtaaaaaa
    11701 gggcagcgtg tatggaccgg tggcggtgat gaagcagcgc tggcgcgcgg tgtttataac
    11761 acttatatcg aggataatct gcgctactcg caaaacgcgc cgctggatat gtataaagag
    11821 gtgaataccg gcaccaactt gccagcgcag atcgatcttt atgccgttga tggcgacgag
    11881 tacaaattcc tctgtatcgc caaaggtggc ggttcggcaa acaagacgta tctctatcag
    11941 gaaaccaaag cgttactgac gccgggaaaa ctgaaaaatt acctggttga gaagatgcgc
    12001 acgctgggta cggcggcctg tcctccgtat catattgcgt tcgttattgg tggaacttct
    12061 gcagaaacga atcttaaaac ggtgaaactg gcttccgcta aatactatga tgaactgcca
    12121 acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg aactggaaaa agaattgctg
    12181 atcgaagcgc aaaatcttgg tctgggtgcg cagtttggtg gtaaatactt cgctcatgac
    12241 atccgcgtga ttcgcctgcc acgtcacggc gcatcctgcc cggtcggtat gggcgtctcc
    12301 tgttctgctg accgtaatat caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa
    12361 ctggaacata atccaggcaa atatatcccg gaagagctgc gcaaagcagg agaaggcgaa
    12421 gcggtgcgcg ttgacctcaa ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat
    12481 cccgtttcta cacgcttatc gcttaacggc acgattatcg tcggtcgtga tattgctcac
    12541 gccaaactga aagagcggat ggataacggt gaagggctgc cgcagtatat taaagatcat
    12601 ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg ttctcttggc
    12661 ccaacgaccg ccggacggat ggattcttat gtcgatcaac tgcaagcgca gggcggaagt
    12721 atgatcatgc tggcgaaagg caaccgcagc cagcaggtga cggatgcctg taaaaaacac
    12781 ggcggcttct acctcggcag tatcggtggt ccggcagctg tattggcgca gggaagtatt
    12841 aagagcctgg aatgtgttga atatccggaa ctgggaatgg aagccatctg gaaaattgaa
    12901 gtggaagatt tcccggcgtt tatccttgtg gatgataaag gaaatgactt cttccagcag
    12961 atacaactca cacaatgcac ccgttgtgtg aaataaacag agccgccctt cggggcggtt
    13021 tttttacatg gcacgaaaga ccaaacgttt gttatcaaat ggtaaataat gagtgagcta
    13081 aaagttgctt aacgaaagca aaacagaaag aaaaaattaa tcaggtgagg agcaggccat
    13141 gaatacagta cgcagcgaaa aagattcgat gggagcgatt gatgtcccgg cagataagct
    13201 gtggggcgca caaactcagc gctcgctgga gcatttccgc atttcgacgg agaaaatgcc
    13261 cacctcactg attcatgcgc tggcgctaac caagcgcgca gcggcaaaag ttaatgaaga
    13321 tttaggcttg ttgtctgaag agaaagcgag cgccattcgg caggcggcgg atgaagtact
    13381 ggcaggacag catgacgacg aattcccgct ggctatctgg cagaccggct ccggcacgca
    13441 aagtaacatg aatatgaacg aagtgctggc taaccgggcc agtgaattac tcggtggcgt
    13501 gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg aacaaaagcc aaagttccaa
    13561 cgatgtcttt ccgacggcga tgcacgttgc ggcactactg gcgctgcgca agcaactcat
    13621 tccacaactt aaaaccctga cccagacgct gagtgaaaaa tcccgcgcat ttgctgatat
    13681 cgtcaaaatc ggtcgtaccc acttgcagga cgccacgccg ttaacgctgg ggcaggagat
    13741 ttccggctgg gtagcgatgc tggagcataa tctcaaacat atcgaataca gcctgcctca
    13801 cgtagcggaa ctggctctgg gcggtacagc ggtgggtact ggactaaata cccatccgga
    13861 atatgcgcgt cgcgtagcag atgaactggc agtcattacc tgtgcaccgt ttgttaccgc
    13921 accgaacaaa tttgaagcgc tggcgacctg tgatgccctg gttcaggcgc acggcgcgtt
    13981 gaaagggttg gctgcgtcac tgatgaaaat tgccaatgat gtccgctggc tggcctctgg
    14041 cccgcgctgc ggaattggtg aaatctcaat cccggaaaat gagccgggca gctcaatcat
    14101 gccagggaaa gtgaacccaa cacagtgtga agcattaacc atgctctgct gtcaggtgat
    14161 ggggaatgac gtggcgatca acatgggtgg cgcttccggt aactttgaac tgaacgtctt
    14221 ccgtccgatg gtgatccata atttcctgca atcggtgcgc ttgctggcag atggcatgga
    14281 aagtttcaac aaacactgcg cagtgggcat tgaaccgaat cgtgagcgaa tcaatcaatt
    14341 actcaatgaa tcgctgatgc tggtgactgc gcttaacacc cacattggtt atgacaaagc
    14401 cgccgagatc gccaaaaagg cgcataaaga agggctgacc ttaaaagcgg cggcccttgc
    14461 gctggggtat cttagcgaag ccgagtttga ccgctgggta cggccagaac agatggtcgg
    14521 cagtatgaaa gccgggggtt aatctgcaac atacaggtgc agccgtggaa tgattaaacg
    14581 aagcggctgc gcctgtggtt tataacggtg ctgcacattg tcggcatcgt aatttaacaa
    14641 ctcaccaacg tccggcacgc ccgcgccatt atcccgatta atcagcgcaa tcagcggcgt
    14701 agggcaggcg tgttgtactt gtttttgatc tcccttatac cagacgcggg caatcggctg
    14761 caccttcacc ggacgtttga tttttaactt cgcgttctgt ggcagggcag cgatatcctg
    14821 atattctcgc tccagttttc tttgccactc ctcgcgcgtc cacggtgcga cactgcgtgg
    14881 tgatttcagg cttttttcca gctgtgccag gacttcatca cgatgcaaat ttttaatgat
    14941 atgtttatta gcccagccaa aacgtaaagt ggcggggtcg tgcagaacgg tgagcgtgcg
    15001 gtaagcatta agggtaatca gccccggaaa atgacgatgt acccattcaa aacgtgccac
    15061 ggaggggagt tctgattcaa ccgtgacgat atgctcgaac gtggtcttga gtttattgat
    15121 gtgctgaata tgactgacca acgctgcttg cgaaaagtta tcgacctggt aacacaacac
    15181 gccaggcaga cggacggcgg ctttgctgct gcgattttcg gactgttgtt gaataaataa
    15241 atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg ccgagatgtt gttttacctc
    15301 aatacgatta agcggattat gctcatcctc tttttttacc tccggcaaag agaacacgcg
    15361 ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat gcagccagct cttgttccat
    15421 ctggcgaaaa gtagtgttga gtcggtctac gagatcgtaa cgcgccataa tattaatcac
    15481 tttagttaca acatccttat tttatagcac agtggtggtg acttgaccac cgtgcgcata
    15541 gtgtcaggca gaggtaaatt gcgggatgtt atgccataac ggccagctga acgagaagcg
    15601 ggcacccccc agttcgctgg tgtcacaatt aaccgtaccg cccattgcca gtgctataga
    15661 gtggacaatg gccagcccca gcccgcagcc gccggttgag cgatcccggc tgggatcgag
    15721 gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg gcaatccctg ggccatcatc
    15781 ctcaacaatt aatgtcgctc tattccccga cagtagcagg ctggtttcaa ccgttgagtg
    15841 acagtagcgc agggcgttat tgagcaaatt atccagcacg cgctccatta agcgcatatc
    15901 caacgcagca taatggcctt gcgcgagcgt ttttatccgt accgttttat cgggcgttac
    15961 tgcctgaata tctgccagat gcgttgacag ccacaacggc aggtctggtt cgctaagatg
    16021 aagctcattt tgtgggcgat cgagccgggc ataagtcagc agctcttcaa ttaaagcttc
    16081 aagttgactg atatcgcgat tcaacgcctg ggattcggcg gcgctcaggt tatcgctcat
    16141 ctccagtcga taacgcaggc gcactaacgg tgttcgcagt tcgtgagcga taccgtcaat
    16201 aagctgtttt ttgctggcaa tcaaggcgtt gatattgtcc gccatctggt taaatgcgac
    16261 gccaagtcgt tcaaagctcg aaccttcatc aaagtggata cgctcactga gatgcccatc
    16321 gccaaatcgt tgcgccgctg cttccagttt taacatatcc tgccagtgcg gacgcatcca
    16381 gataaacacc ggaaaggcga gggaaatggc aataaaagcg atcagggcga tatccagcaa
    16441 tcgcatctga tggaggtaat aaagataagg aacaggacca actgccagca cgtagtggct
    16501 gcgtgggata cgttgcaaaa aagtgtactg atcgtccaga gcgacaattt cgccgccacg
    16561 cagccggtgc atggaaatat catcaagatg atatttactc agcggctcga cacgcagatc
    16621 gaaagagaga tttaaatcca tctctttcag cgtcttaccc cagtcgtgtg gggggatctc
    16681 acgcaattcg ctgcgcatca gatacagcga actgttcatc aaatcatcca gcgactgttt
    16741 gcctgcgcgt tcggcggtaa atttgtacac cagcccgacc agcagagaca tcacaaggaa
    16801 gcagacaaac aataacaggt aaaactggat aaacagtttt ttcatcgctt attcccacgc
    16861 atgaggcgca aaaagatagc ctttgttacg cacggtttta atacgataag gttctgcggc
    16921 gttatcgagc agtttttttc ttaaccgcga aatagccacg tccacgctac gatccagccc
    16981 gtcataactg acgccgcgta aatttttcag caatgcatcg cggtccatga tttgcccggc
    17041 atgggtagct aattcccaca ataattcgaa atcagctgtc gagagcgaga tttcagtgtt
    17101 agccagggag actacgcggt tgatgggatc gatggtcaac gtgccgaaat gcagggcttt
    17161 gtagggagtc agagacgttt cctgaatacc tttggtcact gtggcttgct cattctgacg
    17221 caaatgcaag cgtaaacgcg ctaacaaaac agcagggggc gtcgttttga gaatatagtc
    17281 gcaggcgccc atttccagtg ccaggatgtg gttcatatcg ctatcgagag aggttagaag
    17341 aacaatcggt ccggaccact ttgcgcgtaa atcacgacaa atggtcatgc cgtccttgcc
    17401 tggtagcatg atgtcgagta acaccaaatc cggattttct cgcagaatag tttcttcggc
    17461 ccggtcgccg cgcggctcta cggtaacctg catatcatgt tttgccaggt atgcggcaat
    17521 cagtgcaccg acttccgcat catcttccac aaatacgata gtgttcataa cattcacggt
    17581 agatataaaa acgtcacaat acaccgcact gtttttactt actatcaatc gtttctaaac
    17641 aaggtttcat caggtattct actgatgact tgttatggaa gtgttaaggt aaaaagatgg
    17701 ggctggtaat caaagccgcg ctcggtgcgc tggtagtgct gttgattggt gttttagcaa
    17761 aaacgaaaaa ttattatatc gccgggctga ttccactttt cccgaccttt gcgcttatcg
    17821 cgcattatat tgttgccagc gaacgcggca ttgaagcctt acgcgcaacc atcattttta
    17881 gtatgtggtc gattattccc tattttgttt acctggcgtc gctatggtat ttcaccggaa
    17941 tgatgcgctt gcccgctgct tttgttggct ctgttgcgtg ctggggaata agcgcatggg
    18001 tgttgattat atgctggatt aagctgcatt aacgcagatg acgaccgcca tcaagtggga
    18061 gactgcgtcc ggtgacaaag cagctggtaa gtaagtaatc gaccaggtcg atcacttctt
    18121 tctcgccagg cgcggttttc atcagtgatt tattcagcgc ctgttgtcga tattcggcat
    18181 catcatgttc attaaacagg atcagcgatg gcgcaataga attcactttc acttccggtg
    18241 ccagcttgcg ggcaaacgag cgggtcatat tatccagtgc cgctttgctt gcagcatagg
    18301 caatatgttt gtcgctaccg cgctccacca cataatcggt aaagtgaatg atatcgctgg
    18361 cggcgtgtcc gtgcccacgc agtaatcttt ccagagcatg gttgagcagg tatggggtat
    18421 taacgtggat ctgcatcatg caagccagta cgtcggtcag tggcgctccc ggtttttccg
    18481 ccatccacgc gctggcgtta tgcaaaatag cacgcagacc atgggtgctt tttagtactt
    18541 catcggcaaa cgccatcaca ccgtcgttgg tcgaaaaatc agcctgaata cactgcgcac
    18601 ctgccttaat cagtccatca atggcgggat agtgcgtccg atagctgaca atcaccggtt
    18661 gcttttgatt aatgaaatgc catgcgaggg cgaggccgat gcggcgacct ccgccagtaa
    18721 ttaatattgg caaggactgg gttttaccca tcgttatctc ctttgctatc caacgatggg
    18781 atgaacgtta tcccaccagc atccaggtcg ccggaactga ggcaattaac agcatgccaa
    18841 tcagcaccat ttcctgacgg ttcagtacat tatcatgtgt atgcgtttta cgcgcgtaca
    18901 ggaaaaccag cagccccggc gcatacagaa caacggataa cagcaagtgc attggaccag
    18961 aggcgtacag taaccataag ccataaatgc aggcaccgac acctaccgcc ttatgcagtg
    19021 gacgggttgc gattttcagc aagaacgcgc cgaccaggaa atagggcacc agaatcattt
    19081 ctgaggcgat ggtcagcaat gtgttgtaat cggaaccggt cagccagatg agcaccagac
    19141 aaatttgcac gcagatgttg gtcagccaca gtgaagcgga tggcgcagct tgtgcgttct
    19201 ggcgcgcaaa aatgcgcggg aatgctttat gagtggctgc aaggaacgga acttctgctg
    19261 ccatgatggt ccagcttaag tacgcgccgc aaacggaaac gatcaaaccg gcagcgatga
    19321 tgatttcgcc ccacggtccc atcatttcca ccatcagacc ggccattgac gggttacgaa
    19381 tttcagccag ttcaggacgt gccaccacgc ccagcgaaag cagcgttacc agcaaataaa
    19441 cgcccagagc ggagagaacc gccagcagtg tcgctttgcc aacatcacgc ttattacgcg
    19501 cacgcgcaga aacaaccacg gcaccttcca caccaatgaa aacccacagg gtgatcagca
    19561 tggtgttttt cacctgttcc cacacgggta cgccaagtgc aagtccggtg aagtcgagct
    19621 tgaaggtatc cagtttgaac atcatcatcg ccagcacaac aaacagaccc aacggcaaca
    19681 attttgccag tgtcgccaca aggttaatgc tggcagcggt ttgcaccccg cgcaggatca
    19741 aaaagtgaac aatccacaat aaagccgatg caccgacgat cgattgccag gtattaccat
    19801 cgccaaacag gcgcaattcc ggcgtgtccg taaaaaagct taacgcggaa aaaacgatca
    19861 ccagatagga gacgttggcg atgaccgcgc acagccagta tccccatgcg gaacaaaagc
    19921 cgattagctc gccaaaccct tcgcgggcat aggtaaagat accgccgtca agttcggggc
    19981 gaatgcgcgt gaggatcagc atggcaaagg ccagcaataa aatgccagcg ccagtaatac
    20041 cccagccgat gagcagtgct gccgggctgg caactgccgc catattttgc ggcagactga
    20101 aaacacccgc gcccagcatt gagcttaata ccagcgcggt gagtgcgctc agtcccagtt
    20161 tcttttccat cgatatccag tgattaaaac gtgacaagaa gaataaatat tagggcaaaa
    20221 cgcataaaaa tggagtttgc cactgaagcg cgggatttta cgaagagatg tgtctgcatg
    20281 caatggtcgc agggaaattt atgactaaaa atgcgaaagg cggcatcaag ccgccttata
    20341 ggagtaactg aatagttatt tatacagatc tgcgctgacg gtcaggttat taccacgttc
    20401 ctgccactgg cgggtgatgt ggtaatactt agcacctttc ttcgcggcac gtttcgcaac
    20461 ctgataggag acttcggtca tgttgccgta gttgccagag aatttgatgc tgtcgaacgg
    20521 gaccatcatc gctgctgtcg ctttgttcag ttcttcgact ttagtgccat ccgggagcgt
    20581 gacggtgtaa cgcccgcctt ttgatgactg ggtttcaaag aagcgaccga cttcagaact
    20641 tggtgatgcg gtagtcgcaa cacccgggat ctcaactttc ttcgcggctt cgccaccggc
    20701 agccagagct gcacgtcctg cttcggaatc tgccgggatt acatccgggc tctggacgat
    20761 acgtttctta gcatcttttt tatagatgaa tgcagtaata cgctggttac cgccctggtt
    20821 ggcatcgatt tgacgaacaa tgtagaaaga gtaggcacct ttcgcttttg ccgctttggt
    20881 gatggcatca ttgacttctg gctggctacg atagaagccc tggacggtga ccgtgtcaaa
    20941 cggttcaatc agaacagcct gatctttcgg cagttcgaca acaccgttaa ttacgcgatt
    21001 acttgtttct tctgcttttt cagcatcggc tttatagagg tcagcgacca cacgccagtt
    21061 accgctgtta ccaaaatcag aagtgtcgac aacataaaaa gaggcggcac cttctttatc
    21121 tgcgcgacga gaaacggctt tcaccgcttc gccaatagca ttaaaacgac cggtaaccac
    21181 tacacggtca aaaggtttaa ccgctgccgc ttgctccggt gtcagttctg ttgctgcgtt
    21241 aacggagaat gccgtagcag aaagcagtgc cgacgccagg agggtgttct taagcttcat
    21301 aaaaataatc cttcgccttg cgcaaaccag gtactggtat tgttattaac gagaaacgtg
    21361 gctgattatt gcatttaaac ggtgtaactg tctgcgtcat ttttcatatc acattcctta
    21421 aaccaatttt aattctgctc aaatgaccgt ctatgcttaa aaaacagccg tatcaccatc
    21481 aatactactg aagcaactga attgtataag ttaatttaat gttaagtagt gatttgtgcc
    21541 ggggcgatgt ctcgttttac ccggccgccg aagacaatta tcagtcttta tccggcgttc
    21601 taaggtgttt atcccactat cacggctgaa tcgttaatat tttgcgagtt cacgccgaaa
    21661 tactgatttt tggcgctaga tcacaggcat aattttcagt acgttatagg gcgtttgtta
    21721 ctaatttatt ttaacggagt aacatttagc tcgtacatga gcggcttgtg tggctcctga
    21781 cacaagcaaa ccatcatcaa taaaaccgat ggaagggaat atcatgcgaa ttggaatacc
    21841 aagagaacgg ttaaccaatg aaacccgtgt tgcagcaacg ccaaaaacag tggaacagct
    21901 gctgaaactg ggttttaccg tcgcggtaga gagcggcgcg ggtcaactgg caagttttga
    21961 cgataaggcg tttgtgcaag cgggcgctga aattgtagaa gggaatagcg tctggcagtc
    22021 agagatcatt ctgaaggtca atgcgccgtt agatgatgaa attgcgttac tgaatcctgg
    22081 gacaacgctg gtgagtttta tctggcctgc gcagaatccg gaattaatgc aaaaacttgc
    22141 ggaacgtaac gtgaccgtga tggcgatgga ctctgtgccg cgtatctcac gcgcacaatc
    22201 gctggacgca ctaagctcga tggcgaatat cgccggttat cgcgccattg ttgaagcggc
    22261 acatgaattt gggcgcttct ttaccggaca aatcactgcg gccgggaaag tgccaccggc
    22321 aaaagtgatg gtgattggtg ccggtgtcgc aggtctggcc gccattggcg cagcaaacag
    22381 tctcggcgcg attgtgcgtg cattcgacac ccgcccggaa gtgaaagaac aagttcaaag
    22441 tatgggcgca gaattcctcg agctggattt taaagaggaa gcgggcagcg gcgatggcta
    22501 tgccaaagtg atgtcggacg cgttcatcaa agcggaaatg gaactctttg ccgcccaggc
    22561 aaaagaggtc gatatcattg tcaccaccgc gcttattcca ggcaaaccag cgccgaagct
    22621 aattacccgt gaaatggttg actccatgaa ggcgggcagt gtgattgtcg acctggcagc
    22681 ccaaaacggc ggcaactgtg aatacaccgt gccgggtgaa atcttcacta cggaaaatgg
    22741 tgtcaaagta attggttaca ccgatcttcc gggccgtctg ccgacgcaat cctcacagct
    22801 ttacggcact aacctcgtta atctgctgaa actgttgtgc aaagagaaag acggcaacat
    22861 cactgtggat tttgatgatg tggtgattcg tggcgtgact gtgatccgtg cgggcgaaat
    22921 tacctggccg gcaccgccga ttcaggtatc agctcagccg cagacggcac aaaaagcggc
    22981 accggaagtg aaaactgagg aaaaatgtgc ctgctcaccg tggcgtaaat acgcgttgat
    23041 ggcgttggca atcatccttt tcggctggat ggcaagcgtt gcgccaaaag agttccttgg
    23101 acacttcact gtgttcgcgc tggcctgcgt tgtcggttat tacgtggtgt ggaatgtatc
    23161 gcacgcgctg catacaccgt tgatgtcggt caccaacgcg atttcaggga ttattgttgt
    23221 cggagcactg ttgcagattg gccagggcgg ctgggttagc ttccttagtt ttatcgcggt
    23281 gcttatagcc agcattaata ttttcggtgg cttcaccgtg actcagcgca tgctgaaaat
    23341 gttccgcaaa aattaagggg taacatatgt ctggaggatt agttacagct gcatacattg
    23401 ttgccgcgat cctgtttatc ttcagtctgg ccgggctttc gaaacatgaa acgtctcgcc
    23461 agggtaacaa tttcggtatc gccggtatgg cgattgcgtt aatcgcaacc atttttggac
    23521 cggatacggg taatgttggc tggatcttgc tggcgatggt tattggtggg gcaattggta
    23581 tccgtctggc gaagaaagtt gaaatgaccg aaatgccaga actggtggcg atcctgcata
    23641 gcttcgtggg tctggcggca gtgctggttg gttttaacag ctatctgcat catgacgcgg
    23701 gaatggcacc gattctggtc aatattcacc tgacggaagt gttcctcggt atcttcatcg
    23761 gggcggtaac gtttacgggt tcggtggtgg cgttcggcaa actgtgtggc aagatttcgt
    23821 ctaaaccgtt gatgctgcca aaccgtcaca aaatgaacct ggcggctctg atcgtttcct
    23881 tcctgctgct gattgtattt gttcgcacgg atagcgtcgg cctgcaagtg ctggcattgc
    23941 tgataatgac cgccattgcg ctggtattcg gctggcattt agtcgcctcc atcggtggtg
    24001 cagatatgcc agtggtggtg tcgatgctga actcgtactc cggctgggcg gctgcggctg
    24061 cgggctttat gctcagcaac gacctgctga ttgtgaccgg tgcgctggtc ggttcttcgg
    24121 gggctatcct ttcttacatt atgtgtaagg cgatgaaccg ttcctttatc agcgttattg
    24181 cgggcggttt cggcaccgac ggctcttcta ctggtgatga tcaggaagtg ggcgagcacc
    24241 gcgaaatcac cgcagaagag acagcggaac tgctgaaaaa ttcccattca gtgatcatta
    24301 ctccggggta cggcatggca gttgcgcagg cgcaatatcc tgtcgctgaa attactgaga
    24361 aattgcgcgc tcgtggtatt aacgtgcgtt tcggtatcca cccggttgcg gggcgtttgc
    24421 ctggacatat gaacgtattg ctggctgaag caaaagtacc gtatgacatc gtgctggaaa
    24481 tggacgagat caacgatgac tttgctgata ccgataccgt actggtgatt ggtgctaacg
    24541 atacggttaa cccggcggcg caggatgatc cgaagagtcc gattgctggt atgcctgtgc
    24601 tggaagtgtg gaaagcgcag aacgtgattg tctttaaacg ttcgatgaac actggctatg
    24661 ctggtgtgca aaacccgctg ttcttcaagg aaaacaccca catgctgttt ggtgacgcca
    24721 aagccagcgt ggatgcaatc ctgaaagctc tgtaaccctg acggcctctg cggaggccgt
    24781 cactctttat tgagatcgct taacagaacg gcgatgcttt gacctcccgc tgtttgttca
    24841 agcgcaattt tgacaataat tgtcaacggc acggaaagca gcatacccac cggtcctaac
    24901 aaccatcccc aaaaaatcaa cgacaaaaat accaccaatg tggaaagccc cagcccacgc
    24961 cccatgatgc gcggctccag aatattgccg aagacaagat taatcagcag atatcccgcc
    25021 agcaccagca acgcttcgta gaagccatta aacaccagta cctgagcgat aggggggatt
    25081 gccgcgagaa ctgaaccaat attggggatg taattaagcg caaaggccag caatccccag
    25141 acaaaagcga agcgaacatc gagtgcggcg agcatcgccc aggcgaccag gccggtgatg
    25201 atgctgatgg ctgttttcag caccagataa tgagacacgc tgtcaatcgc gcgctgaatc
    25261 gccgccatcc cttcaaccgg acgcgccatc atttgctgaa attttccggg caattgtggc
    25321 acttcgagca acataaacag caccgtcagc agcaataaaa agattgatga catggcatta
    25381 gataactgcg tcaataagtt ggtgagcaac gtcatcgccg cgttcggatc aatgtaatgc
    25441 gccagttggt caactgagac atcaatccct acgcgttgca acaacggttc aatcgcttgc
    25501 agcggcgtca taatagagtt gcgatattgc ggtaacgtcc gcgtcaactc attgagcgcg
    25561 gaccccagat aagctaacag caacaccatc gccatcacga tgatggtcat caaaatcgac
    25621 actgccagta cacgcggcac acgccagcgg accatgtgtt gcaccagcgg gttaagaata
    25681 acagcaataa ataatgcgag aataaacggc acgataatct cggcggcaaa acggataccg
    25741 cagagaataa tgaccagcat tcccaacata atgacgattt ttaggccatt gagcgtgatg
    25801 atcggctttg ccatgcttgt ccccgttttt tcttcctttg ctaataataa tgtctttttc
    25861 gctccttcat cataacgaaa atcaaaccgc tcaggtaaag aagtgaaaat attttgagtt
    25921 aattcttaag ctatgataca aatcaggcgt gttcaactac cgaggacaat tatcatccgc
    25981 gatgacgaga agcaacactg cggataattg taatattatg gacaatttgt tcaggacgtt
    26041 attttctaca tttacccatc ttcgtacctc atccactatc ttgctcgtag gtgagcagca
    26101 ctggcgcaac gcgctttagt cctttcttct gcctaagctt gcgctatgcg cggggcttga
    26161 tcactttact ttggtttcgc tgaattaagc gaaaattaaa ataattctct tgcaggagaa
    26221 ggacaatgta tatttattgg attttattag gcctggctat tgctacggaa attaccggta
    26281 cgctgtcaat gaaatgggcg agcgtcagtg agggaaatgg cggctttatt ttaatgctgg
    26341 tgatgatttc tctgtcgtat atatttctct ctttcgccgt taaaaaaatc gccttaggcg
    26401 tagcttatgc gctgtgggaa ggtattggta ttttatttat taccttgttt agcgttttgt
    26461 tattcgacga aagtttatcg ctgatgaaaa ttgccgggtt aaccaccctg gtggccggga
    26521 ttgtgttgat caagtcaggt acccgtaaag cgcgtaaacc tgaactggag gtgaaccatg
    26581 gcgcagtttg aatgggttca cgccgcctgg ctggcattgg caatcgtgct ggaaatcgtt
    26641 gctaacgtct ttttgaaatt ttctgacggc tttcgtcgca aaatatttgg cttgctctct
    26701 ctggcggcgg tgctggctgc ctttagtgcg ctttctcagg ccgttaaagg gatcgacttg
    26761 tctgtcgctt atgcattgtg gggcgggttt ggtattgccg ccacgttagc cgcaggttgg
    26821 atcttgtttg gtcaacggtt aaatcgtaaa ggctggattg gcctggtgct gctgttggct
    26881 ggaatgatca tggtgaaact tgcctgatga acacgctgcc cgcgctgtcg ggcagcgttt
    26941 gaacattatt tttgcgacag ttgatccagc ttgtcgcgaa aaccggtaac agaaatggcc
    27001 cggttatcgg cgcgccagcg atctttcgcg gcaggagccg agctttgcac cccaattaat
    27061 tgccagccgt catcggtatg caacatcaga ggcgaaccgc tgtcacccgg caaggtatcg
    27121 cactgatgcg acatcaccga cgtttgcgcc cagccagtca cttcacagtt ttgatgactg
    27181 tacaacgtat cgagatgatc ttcagggtag cccgcctgag tcactttacg accagccgct
    27241 tttaatgcgg cggtaagcgc ggctttatct ccctcaaata acggcaacgg tgtaatgcca
    27301 gaagggggat tacgtagcac aatcaaaccg aagtcccacg gcgcagctgc gggaggtaca
    27361 atccagccat ccccatctgc ttttaaccgc tttcccagtg ttggatcaac gcggccttct
    27421 atgtcgtgga tctcatagcg ccaaagacct ttatttgaca cgaaacgcag cgctaccgct
    27481 ttatcggctt taccctttgg cggcgtcaat aaacagtgtc ctgccgttaa tgccagattg
    27541 ggtgcaatca gcgtcgccgt acataaattg ccgctggccg tttccagttg gccaaccgca
    27601 tcccacggtg attgggtcgt gtcattcact ggcacacgat catcatgacc aaaaaacagg
    27661 gtgctgacct catcgtttgc cgatttggca acgtctggtt tatctgcaaa cacaaaagca
    27721 gacgtcaaac taattgcacc caacactaca gcaatggttg tacgcatatc acactctggt
    27781 gggtaattat gattattaaa agcaaaccct cataaatact atagacggga cgggatgaaa
    27841 gtgggagtaa aatcagataa ctacgatcag gaaagataaa aacgggcggc aatcagtgcg
    27901 cataaaataa ggatgataag aatgaactca aagcgataac ggcccaccat actaccctcc
    27961 gctaaaggcg gtgctaaacg ttacccgctt agcaccgtaa ttttcaggcg cgagggggcg
    28021 cgccagcatt actgttgaaa acttacgctg cgggttgtgc agcaggtttt gccggttgct
    28081 gatggctgtg tttcttggcg tgtttcttcg ctgcctgcgc tttttgttca ggagctttct
    28141 gttcagcttt ggcattttta tgatgctttt tagccgcctg cgctttctgg gcaggtgctg
    28201 ctttatgctg ttttttatga tgtgtggttt tcgcaggcgc tgctttggtg gtcgtcgcag
    28261 tcggagcagg tgtggtcgca gtctctgcag caaaggcagc agaagacaga cccatagcag
    28321 cggcaacaac cagagctaat acttttttca ttgtcatacc ctcaatttgt tttttcattt
    28381 aaccccactg cggggccgtt gaaataacta tatccctggg aatggcagac ttccgtgagt
    28441 ggttggtttc agcgtgtaac gatatgtaca aacgctgaat aaattacagc attgataata
    28501 acgtattgtg acttaaggaa aatttagcta ccaataaagt agtcttgatc gtgttaacta
    28561 ttttactact tacaaagcgg tagaataact gcgcatcagt aataaatgac acacagcaaa
    28621 atgaatccgt ttatttgggt acttataatc ctgatgacgc tagatgcgct gcgggaattg
    28681 gctggcgctt cgtctatttt aggatggcta ttaacgttgg tttgagcagg caataagtcc
    28741 ggacgggtat ttacctcagt ccggacttat tttttcaggc gtgcagacga tgatgcaaac
    28801 gcgtcccgac cagcagagca atgaccagca tcagagcaat aaatgcgccg acgccgttcc
    28861 agccatagtt atgccagaaa acaccaccca gcgtcccggc aatactcgac cccagatagt
    28921 aactgaacag atacagcgag gaggcctggc ctttagcgcg ttttgcgcgg gggccgatcc
    28981 agctgctggc aacggagtgg gctgcgaaga atccggctga gaagagtaac attccggcaa
    29041 agatcagcca cagcgagctg aataaggtca tcagtaaacc aaacagcata acccccgtcg
    29101 aaaacaacat caccggaccg cgtccatagc gggcggtcat ggttccggct ttgggtgagc
    29161 tccatgtacc ggtcaaataa gccagcgata ataagccaac cacggcctga ctgacatgcc
    29221 agggggaaag catcaaccta tagccgatgt aattaaacag cgtgacgaac gaccccatca
    29281 gcaaaaagcc ttctgcgaac aataacggta atccccggtc acgccagtgc agacgaaagt
    29341 tgataaataa cgtcttaggg cgcagcgaag tcgggcgaaa atggcgtgat tcagggagga
    29401 ttttccagaa catcaacgcc gaggccagcg cgaaacaacc gattgccgcc agagcaattc
    29461 gccagttaaa aaagtccgtg aagacaccgc taattaagcg tccgctcatg ccgccaattg
    29521 agttgccgct gatatacaac cccattgaaa aggccacgaa actagggtgg atttcctcgc
    29581 taagataagt catgccaacg gctgccacgc cacttaacga aagcccaatc aaggcgcgca
    29641 taatcaaaat gccgtgccag ctggtcatca ttgtcgaaag taacgtacaa atggaggcca
    29701 acagtagcgc cgtgaccatc actggtttgc gaccaatggc atcggatagc gggccagtaa
    29761 acagcaaacc gatagccaac atcgccgttg aaatggacag tgaaatacta ctgttcgcag
    29821 gcgttaaacc aaactcctgc gaaagcaccg gaaggatagg ttgcacacaa tagagaagtg
    29881 cgaaggttgc cagtccggca gagaaaagcg ccagggtgac gcgcataaat tgcggcgtac
    29941 cgcgtttaat aaaatgattt ggctgagaaa tgctttgctt gtcagtgtcg cttgccggag
    30001 cgccatcaac agttgtagta cggctcactt gaaatccttg ctaaatatgc ctgtggatca
    30061 ggcttatgaa tagattagga aaatcgaatt gttctgtcta atatattaat aatctcaaat
    30121 aagatgtttt aaatatgaat attgaacttc gtcatctgcg ttactttgtt gctgttgcag
    30181 aagagctgca tttcgggcgc gccgctgccc gcctgaatat ttcgcaacca ccgctaagtc
    30241 agcagattca ggcgctggag caacaaattg gtgcccgact gctggcacgg accaatcgca
    30301 gtgtattgct gacggaagca ggaaaacagt ttcttgtaga tagtcggcaa atcctgtcta
    30361 tggtggatga tgctgccgct cgcgctgaaa ggctgcatca gggcgaagcg ggggagttgc
    30421 gcattggttt tacttcgtcc gctcctttta ttcgggcggt gtccgatacg ttatcgctgt
    30481 ttcgccgtga ttatcccgat gtccatttac aaacccgcga aatgaacact cgcgagcaaa
    30541 tcgctccgct cattgaagga acgctggata tgggattgct gcgtaacaca gcgttaccgg
    30601 agacgcttga acacgcagtc atcgtccatg aaccgcttat ggcgatgatc ccgcacgatc
    30661 atcccctggc aaataacccg agtgtaacgc tggctgaact ggcgaaagaa ccctttgtct
    30721 tttttgatcc gcacgtcgga acagggctgt atgacgatat tctcgggctg atgagacgtt
    30781 acaatttgac gcccgtcatc actcaggagg tgggtgaggc aatgaccatc atcggtctgg
    30841 tttccgctgg tctgggtgtt tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg
    30901 aaatgcgctg ggtgccgatt gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc
    30961 cgaaacatca tgaacaaagt ccggctgcgc gtaactttcg tattcatctg ctgaatgctc
    31021 tcaggtgagg gaaatttcag cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa
    31081 gtaaaaattt gacgacacgt attgaagtgc ttcaccatag cctacagatt atttcggagc
    31141 gcgaaaatat agggagtatg cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat
    31201 aaagcaaacc aacgcgggcg cagtttatcg cctgattgat cagcttggtc cagtctcgcg
    31261 tatcgatctt tcccgtctgg cgcaactggc tcctgccagt atcactaaaa ttgtccgtga
    31321 gatgctcgaa gcacacctgg tgcaagagct ggaaatcaaa gaagcgggga accgtggccg
    31381 tccggcagtg gggctggtgg ttgaaactga agcctggcac tatctttctc tgcgcattag
    31441 tcgcggggag attttccttg ctttgcgcga tctgagcagc aaactggtag tggaggagtc
    31501 gcaggaactg gcgttaaaag atgactcgcc attgctggat cgtatcattt cccatatcga
    31561 tcagtttttt atccgccacc agaaaaaact tgagcgtctg acttcgattg ccataacctt
    31621 gccgggaatt attgatacgg aaaatggtat tgtacatcgc atgccgttct acgaggatgt
    31681 aaaagagatg ccgctcggcg aggcgctgga gcagcatacc ggcgtaccgg tttatattca
    31741 gcatgatatc agcgcatgga cgatggctga ggccttgttt ggtgcctcac gcggggcgcg
    31801 cgatgtgatt caggtggtta tcgatcacaa cgtgggggct ggcgtcatta ccgatggtca
    31861 tctgctacac gcaggcagca gtagcctcgt ggaaataggc cacacacagg tcgacccgta
    31921 tgggaaacgc tgttattgcg ggaatcacgg ttgcctcgaa accatcgcca gtgtggacag
    31981 tattcttgag ctggcacagc tgcgtctcaa tcaatccatg agctcgatgt tacatggaca
    32041 accgttaacc gtggactcat tgtgtcaggc ggcattgcgc ggcgatttac tggcaaaaga
    32101 catcattacc ggggtaggcg cgcatgtcgg tcgcattctt gccatcatgg tgaatttatt
    32161 taacccacaa aaaatactga ttggctcacc gttaagtaaa gcggcagata tcctcttccc
    32221 ggtcatctcg gacagcatcc gtcagcaggc ccttcctgcg tatagtcagc acattagcgt
    32281 tgagagtact cagttttcta accagggcac gatggcaggc gctgcgctgg taaaagacgc
    32341 gatgtataac ggttctttgt tgattcgtct gttgcagggt taacattttt taactgttct
    32401 accaaaattt gcgctatctc aatttgggcc aggaaagcat aacttagact ttcaaggtta
    32461 attattttcc tggtttatat ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt
    32521 tctttattac cggtacagac acttctgtag ggaaaacggt ggtttcccgc gcattgctac
    32581 aagcgttagc ctcccaggga aaaacggttg cgggatataa acccgtagcg aaggggagca
    32641 aagagacacc cgaagggctg cgtaataaag atgccctggt gttgcagagt gtttcaacca
    32701 tcgaactgcc ttatgaagca gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg
    32761 cgcacagttg cccaatcaat tacaccctca tttcaaacgg cctggcaaac ctgaccgata
    32821 aagtcgatca tgtcgtggta gaagggactg gcggctggcg cagtctgatg aatgatttgc
    32881 gtccgctctc tgaatgggtg gtgcaggaac aactgccggt gttgatggtt gtcggtattc
    32941 aggaaggttg cattaaccat gcactgctaa cagctcaggc gattgccaac gacgggctgc
    33001 cgctcattgg ctgggtggct aaccgaatca acccaggact ggcgcattat gcggaaatca
    33061 ttgatgtgct cggtaaaaaa cttccggcac cgctcattgg tgaactgcct tatctgccgc
    33121 gcgccgaaca gcgtgaactg gggcaataca tccgcttagc tatgcttcgc agtgtgctgg
    33181 cggtagatag agtcacggtg taacgtccgc gaaattaccg acgcaattac gcaggcaatc
    33241 aataaaccgg ggagtagctg atactccccg gtcatttcac atatcatcaa cgtcgacata
    33301 gtcggcgcgt gcgtggtcgc cgccaacagt gtcgccatcc cggtcaatct gagtaaaagc
    33361 gtaatttctt cgccatcagg taaccataat cccaggctac gaccatacaa catgccaatg
    33421 gcaagaccga taaatagcgt cggtgtaaag acaccaccgg gcgcgccaga accactactc
    33481 gccagcacgg cacacagttt acagaggaag atcccggcaa tgatcattaa cagtggcgga
    33541 gcggttaaaa aggattgtac ggtgctatag ccgttgcccc acactgcagg tgtgaacagg
    33601 gaaagaagac ccacgatcaa cccgcccagt gccagttgcc agggcggcgc aagcttgaga
    33661 ctcacaaatc cacgatgaca ggcgttcatt aacgttaaca acagtggtcc gcacagacct
    33721 gccagcacac ctgtactgat aatcagcgca tagtcacgag cctgaaccgt cactgaaagt
    33781 tgtacgctgt agagtaatgc gtcgctatga ttaataagat tgctaaccag caacgccacg
    33841 acggcggaaa taatcaccgg gccgagagag gccaacatca tagtgccaaa cagcacttcg
    33901 gctataaata aactaccagc aagcggggcg cgataggccg cagccattcc cgccgcagct
    33961 ccacaggcga tccataattt ccactcctgg cgtggcgtaa aacgttgggc aaaacacgag
    34021 gctgcaaggg cagctaaaag aatcatcgca ccttcgcgac caattgcact gccgctggtt
    34081 actaccagca gagaggcaag cgatttaacc agacttgctg cgtaatcaaa ctgaccatcg
    34141 gtttgcaacg cttccatgta atcggtcggc gcatgagggc gttgctgggt aaatttctgc
    34201 cagcccatca gcaacaaacc cgccgccagt ccgccgagcg ccggggttag cagccgtcgc
    34261 cacggggaaa gatttgtcgc tgcattgacc agactgccag agtcattatt gaggaacagc
    34321 cactccagta gcagcatcgc atgacgaaac ccggcaacgg caaaggccgc aagaataccg
    34381 acgattgttg cgataagcag acggcggaac atagtgcgca gatccggata gatgtgtaga
    34441 tgatgcatga gcagaaatgg gcaatgaaac acgggcgact attttgcggc aaaagaggcc
    34501 agccgcaaaa gcagtgtgtg ctttaagaga taaaaaaaac gtgacactgt aacctattaa
    34561 ttgccgaatt gcgtgtaatc gtcaccatcc ggcaataata cggtgatcct atcgaaacag
    34621 cggtttaacc gcgacaggaa tcaacagttg tgattgccat tgcgccagcg ttaagcgagc
    34681 gagttcaccc agtgcacggt agaaagggtg ttctgctttt tcgataaaaa cgtcaagaaa
    34741 acgtgttgac cacggaaaaa ggtgccatgc cagcaattct tcgcattctg tctggcgacc
    34801 attctccgcc aaccacgcag ccatcagcag cagtgaacca aaattatctt ccggttcgtt
    34861 ttgcttcatt tcgaactgaa tgcctttctc gcgcatccac tgacgaagtg ccaatgttga
    34921 atcgccaaac agcacagatt cgcgatccag ccagaccgat ccccacggcg gagacggcag
    34981 tgcccacggg ccgacaaaca aacgctgcca ggcctgggcg tgagtctctt cacattgtgt
    35041 ctgaaaagcc gtcaccagcg gcgctaatga cgcttctggt agaggccact gagtttccca
    35101 gccgtcattg gtgagtaccg caaccagagg tgcggcttcc gcactctctg gagcgtaata
    35161 aaacagcgcg cccaacacgc gcgccgcaac agaaaaatta tcttgctgtg aaaaatgggt
    35221 cattcctaac atcctgaaat gtgcgggttg ccccgcacca atttgttaac ctgcaatagc
    35281 cattcctacc gtcatgtgca ggccataaaa gagcacacgc ccgatcatct caccgccaag
    35341 aatgaggatt agccccagaa caagtccggc aacgtgaggt tcacggcgac gaatgagcgg
    35401 acaaagccag catccaagac ctgcgcataa caataccaca cgccagacct gtaaggaggc
    35461 gtaatctggt accagtgcgc tggcttgttg tacggatgag tgaatagacg ccagagacaa
    35521 accttgcagc acaatcaacc cggcacaagc gatcagcgcc agaatgctaa tgatggcaaa
    35581 tggtgtggta ttaaatgtaa cgcgggctgc acgcagaatt gtggcagcaa gtattggacc
    35641 gctcaacaat accgtcagga agaaagccag cgtagtgtaa ccgttatgcc aggttggcac
    35701 agtgtcgatt tgatacacgc aggtcatcat ccagacgaaa atgatgccga gtgccatgct
    35761 gacaagtaac cagagtttcc ccaacgcttg cggcatttta ccgatgaccg ccaccagcca
    35821 ccataatccg ccaacggcaa aaaatatgga accggcagca atctcgttac tcagaccaga
    35881 agctccgatt ctattcagcg agttaaacgc ccgcagtggc gatcccaggt gcatgacaga
    35941 ggcaataaac ccgatgccca tcaacagcca gaggaaaaac atgccacgaa taatgtgctg
    36001 acggtctgcg tcatttttgg cggcgaacca gccaattccg ctaacaatca gcgcgcctac
    36061 tacgcattga cccaatacgg taaagataac cagtggccat tcatgccatc catttcccat
    36121 tttacacttc ctccggatta gccagataac cagtggtatc gccggtcggg cggctgttgg
    36181 cgttaggttt gataacgata ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg
    36241 cagccagagt gccgtgcttc tggcgcaatt cttcaattgg accgaactca agtgcacgca
    36301 gtgggcagga ttcgacacat atgggttgtt tcccctcggc gacgcgcgaa taacaaccat
    36361 cgcacttcgt catgtgccct ttttcagcat tgtactgtgg cgcgccgtac ggacaagcca
    36421 tgtggcagta gcggcagccg atacaaacat cttcatcgac caccacaaag ccatcttctc
    36481 gtttgtgcat tgcgccgctg gggcagactt tggtacacgc cggatcatcg cagtggttgc
    36541 acgaaattga gagatagtag gcaaagacat tctgatgcca gacgccatta tcttcctgcc
    36601 agtcgccgcc agcatattca taaatacggc ggaaactgac ttccgggcca agatctttaa
    36661 aatctttgca cgccagttcg caggttttac agccagtaca gcggctggaa tcgataaaaa
    36721 atccatattg tgtggtcatg ggctactcct taaacctttt cgatctggac aagattgctg
    36781 tgcgacgggt ttccctttgc cagcggtgaa gggcggtgag aggtcagaat accgttaact
    36841 gcagtggata ggtcccccgt aggggatcgt tccaaccgtc gaaacctggg gtatacgctg
    36901 gcagtggatg aataatttca tcttttttca attcccaggt atccgcaatt gtcgccagtc
    36961 gttcagaata aatttcgatt tttcccgaag gtgttttcaa cgggtttgcc tgtggatcta
    37021 cacggaatgc gcggaaagcg acgtagtgtt cttccgggca ttttttctta aagatcccgg
    37081 tcgttttcat ctcctcgtag tcgggcatct cagggttacg ttccttcgtt ttcgcatgga
    37141 gatatttgat ccattcatgc tgactgcgac cttcagtaaa ggtttgataa acgtctggtc
    37201 ctaagcgttt ggcgacttca ctcagcatcc agtagatggg tttgcgttca aattttgctg
    37261 aggttgcggg ttgggcgagg atcacatagc ccatattccc ggcagattca tgagagataa
    37321 ggtcttcttg ctctgttggc atcaggtcgg gtaacaggat atcgcaatac ttagccgagg
    37381 cagtcatgaa gtggtcaatg ccaacaatca tctcgcactt gctgtcatcc tgaagcacct
    37441 catgggtgtg attgatgtcg ccatgctgat tgatcaatgt gttactggcg tagcaccata
    37501 aaaacttgat ggggacatcc agtttttctt ttcctcgaac accatcacgg gtcgcggtca
    37561 tttccgtacc atggtcgatg gcatctgtcc atgtaaagac ggaaagccgc tcatccgcgt
    37621 ttctgccggg ttatttccga acataacgac cagtttcgta ttggcgatat catccgggct
    37681 gttgccatca ttggcaccga acatataact cattgcggca ctgatctgtg cggtactgta
    37741 gctgccatag cgactgagaa aaccaccgca agagttcatc agacggtacg ggacgtttga
    37801 gttggtgatg tttccgccat ctacgcctgt tccatagaga acatgtacag cctcattgcc
    37861 gtaatctttt aggatccgcc gaagattatc actgatggta tccagggctt cgtcccaact
    37921 tatccgttca aatttacctt caccgcgctt gccgacgcgc ttcatgggat atttcaacct
    37981 gtcaggatga ttcatccgtc ggcggataga gcgcccgcgt aaacatgccc gaacctgatg
    38041 attaccgtaa acgtcgtcac ctgtcgtatc agactccacc cagtacacgg tgtcatcttt
    38101 cacatgcaaa cgtaacagac agcggctccc gcagttaacg gtgcaggaac tccagaccgc
    38161 tttctcttct accggtgcct gtgccgctcg gaccatttgg gaaaacggca aagtgaaagc
    38221 actgcttgcc agcgcgagac tgccaagtgc ggaggttttc atcagacttc tacgactgat
    38281 ttcagccgac atgagcgcct ctgtgttatg gattttcatc attactcact tattgctttt
    38341 caaacaaaat gtcatgtcaa aatttatggc cgtagtgggt tatatttttt cgatctcgac
    38401 cagattagtg tgctgcgggt ttcccttcgc cagtggtgaa gggcgcagag tggttagcgt
    38461 attcacacag ccgccgtggt cgattttatc gccagacata ttggcctcgt gccaggctcc
    38521 ctggcccata gcactaactc cagggagaat gcgtggtgtc actttggctg gtagccgaac
    38581 ttcgccacga tggttaaaca cccgcaccat atcgccgttg gcaatcccac gtttctgtgc
    38641 atctataggg ttgatccaca cttcctgacg gcaggctgcc ttcaggacat caatgttgcc
    38701 gtaggtcgag tgcgtacggg atttgtaatg gaaaccaaac agttgcagtg ggaaggtact
    38761 acgttcaggg gagtcccacc cttcaaaggt tgaggcataa accggcagtg ggcttatcac
    38821 ttcatctttt tccagttccc aggtacgggc aatttccgcc agcttgctgg aataaatttc
    38881 aatcttaccg gaaggcgttt taagtggatt tgcctcgggg tcgtcacgaa atgctttgta
    38941 ggcgacaaaa tgaccattgg gatctttacg cttatagata cccatttttt ttaattcgtc
    39001 gtaagacggt aacgccggat ctttggcaag cattttggcg tacagatgtt gtaaccattg
    39061 ttcttgcgtg cgaccttcag tgaacttttg atagacgtca ggtccaagac gtttcgcgac
    39121 ttcactcagg atccagtaaa ttggtttgcg ttcgaatttt tcgctggtga caggctggag
    39181 gaaaatgaga tatcccatgt taccggcgta gtcgttagga ataatatctt cctgctcaac
    39241 ggtcattagg tctggcagca gaatgtcggc atattttgcc gatgaggtca taaagttttc
    39301 gatgaccaca atcatttcgc atttcgattc gtcctgcaga atttcatgcg ttttgttgat
    39361 gtcagaatgc tgattaacca gggtatttcc cgcgtagttc cagatggact ccactgtcag
    39421 acggatggac ggacttgaag tagttgactc aacgctactt aaggtcctga tatgactgac
    39481 attaaccatg acaacactgt atatcaatcc agttctttct ctgggtctcc ctatctgtat
    39541 cagcaagtct acacctcaag tcctgacaca aggctgacac aaatcaatct actcagcgta
    39601 gctaagcctc agttagtccg tagagccaat ggtcgatata ctatccgctt cagactcaag
    39661 ggacagacca tcccgtttct ttcagtatcg acccgaagca cagacaggag agtagccaca
    39721 atgcgtcaga gagagcttgc agccacagct aagtccttca tgttggatag gcctgatgtc
    39781 tcactacagg agcttacaga gcatctccgg tctatggctg agcagttcct gacagacgcc
    39841 agtgatgact actggaatgg acttgaggtc gctactctgg tcgacgagaa gtccaacctg
    39901 aaggagctag ccgctacgca agccttgagt ctggaccagc agaagggcat aaggttagcg
    39961 cttgaagtcc tgaccgcagc acagcagagg gtagacactg gtgatacctc tggtctgatt
    40021 aagctgattg atgacaataa ccatactgat tactcaacta taggcgactc tacgtcaatt
    40081 ctgaagaatg agcaggggga tagaccagca gttttcacac aggagcgtca gccatctgta
    40141 gttttttcaa gcctagtgtc ctcgctgctg gctgagaagg tccagaccct gaagtcctcc
    40201 agctacaagg acctgtcgtc ctcactgaat accgtctctc ggttcttacc tgaagacatg
    40261 gacctgatgt cccgctctga gtggctggct gtcagggact ccatgctggc tgctgaggtg
    40321 agaccatcga caatcaacaa gctgctgact aaggcgaaga tgtgtcttga ttatggcctg
    40381 atgaatgggc aacttgaggg ccgcaaccct attgaacgga tgaagctgac caaggatatc
    40441 gactccaagc gcagagcgtt cacagacgag gagctggaga gacttctggt ccgtgttgag
    40501 gctgagtatc agttcacacg acacacggct cacacgacct ctgaggcccg tagatgggcg
    40561 tctctggtgt ctgtggtcac tggcgctagg tctgctgaag tctgccacct gactaaacgt
    40621 gacattgtaa ccttggacaa tgggctggtc tgtatcgaca tcaatgaaga tggggacggt
    40681 aagtctgtga agaacaaaca ttcagtccga cttgtgccgt taaccgatgg ggtctgtggt
    40741 ttcagcctga agtccttcct caaatgggta gacactcagc ctgctgatgg tcccctcttt
    40801 ggtatgactc cgagtgccta ctctagttgg tttaactcta gggtcctgac tgaggcccta
    40861 ggagacgctg agaacgtttc tctccacagt ctgagacatt ggctggcgac ccgaatgaaa
    40921 gagcgtggag tcaatctggt ggacgctcag ggcatcttag gtcacagcag tcagtcaatt
    40981 acgtatgacc tgtacgggaa aggacacgct gtgggtaggc tggctgatgt gcttaagaca
    41041 gctttacttt aagtagggag acccaaggtg gagaccatat acggcttgat ggttaagacc
    41101 ttgggtcact gaagtctgac tgaagttagg gagggtgata ataatcataa tccctcctga
    41161 ctataggccc actggttcgc cttcggctgt ccgactctaa gtctgtctgg tgtcttattt
    41221 aaactggttc cctaactcag gtcctccaac ttggtgtctt atttaaactg gttccctaac
    41281 tcaggtcctc caacttggtg tcttatttaa actggttccc taactcaggc ccctccaaat
    41341 ttcgttgtct gtcagcgact taaatatccc ctcaccttag aagagtttag ggttcagacc
    41401 cttaactatc accttaggag agacgggccg tcctccaccc aatcaaaacc ttaagaggag
    41461 gaccatacat ggctattaaa attcagtgct acaccgagtc cgacaatgcg gctttcttcg
    41521 gtgacgattc cgacctccag caccagctac cagcaccgac atatgacgaa cctaaggccc
    41581 gcacaggtaa gcctcctgtg gtcatcgaca accgggctgt ccgtgcgtgg ctgattgaga
    41641 ctgggtgtct ctcctatacg cagcttgttg aggcgtaccc gtgggctgac gatgtgttca
    41701 caggactctc acgggctacc agtggcgctg tcggtgctgg actcccggta tctaaggcgt
    41761 acctgttctc tgccttatat ggcaccaacg acatcttctc ccgtaacaca gccaaatcct
    41821 acgctgacat cactgggcgt tcaccgtctc ctcgctacct gcgccacgtc aatagcgtgc
    41881 ttcaggctac ggctgtgaat ctggctaagg ttctgaagga ccgaggggat ttcacatggt
    41941 gggattacaa cgatcagtgc ctgtcctgag agccgcgcca gctctgggct ggaatacccc
    42001 tcactatagg aacacccccc taaactatca ctatagggag accagcctcc gcctctgagt
    42061 ctgatgcgtc agaacatgcg agcatctggt caaccgacat taagtatctc tcctaagtaa
    42121 tcaaggcccg tgaccgcctc tgacagacct cctgtatcga cctccagctc gttagcgttc
    42181 tttagtcgtc tcggttagta gctcgtcctg acatgcggcc tgcaaccttc gtctctatgg
    42241 tcgaagaact taacgcgctc aaaactgagc agtcctcaaa agaggaaaat ttccttttat
    42301 tagccgctat ggtcatctct ggtcttcaca cgacctcatc aatcctcaag tctttctgta
    42361 tgtctcctgt tgcctgtgag tctccctatc gttgggggct tgagtaggct ttcagggtgt
    42421 gacttcagga ggcataacaa aaggagtaca acgatatgtc aactcaaccc ttaatgaagc
    42481 atgaagtagt tcttcctgtc atcgttgacg gctatcactg ggcctgtaat caggaccgta
    42541 tgtggaacct gaacgagcta cataaagagc ttgagcttcc atctgctaag gagccgggtc
    42601 agtggcgttc tagcgtcagg acgcttcttg accaagatgc aaatttgcat gttgctcatg
    42661 gtggttcgac tccggggaca tgggctactg aagaggcggc tattgcctat gcgatgtggg
    42721 tctcacctga tttctacctt aaggtcatcc gtgctttcat cgctttacgt aacgacaaag
    42781 tgtcggaagc taaggcgctg gcgcaggact ccaaggaact taagggtctc aagcctatag
    42841 cccgtaactg gctggaaaag ctggataacc cgaagcaggg ccacaccctg acagagtgtc
    42901 ttaagaatct cgacttcagg ttaggtacaa agctggtatc agccaaggct cttaatgccg
    42961 tattgaagtc agggcgtata gctaatccct tctatagtcg caagctggac ggtcgaggac
    43021 agcctgtatt tgacatctcg tcaggtggct ggatgaccag cttcaatcct aagggtctgg
    43081 agaacggcta ttaccgtctt caggataacc actataacgg tcaggagggg cttaaggtcc
    43141 tcaccaaggg ttacgagtgg cttaagtcca acaaggtcga gctggttcgt ctgtgcgcta
    43201 agggaggact ctaagatgat tgacccacgc acacctgagg gccgcatgac tctccgttac
    43261 cgtggctacc ggactgaagt cctcctcctc agagagcttg gtctagaccc ggaagatgag
    43321 acccgccagc accaatcccg agacgagctg atagcgcaac tggtcgctat gaaactgtct
    43381 gcctaatttt aaaacctttt gagatgaccg atgcggggag acccgtaggc gctactgcgt
    43441 ctgcgatacc gatagtctca actctcaaga ggagaattac ctatgatgtt caacaacaac
    43501 aactggaagc tgtcagtcac cgacatcaac ctttatgaaa acactgtgag tctggacggt
    43561 cagtcctatc cgttgtcgct cgctatcaag accctgattc cgggctacct gtcaggactg
    43621 ccgtctacca gccgtgagtc tatggagtta ctggaagctc tggctgaagc tggtgtagct
    43681 attggcaact tcttcagtga tgacctgatg accgcctatg gtcgccgcca gcagaacaag
    43741 cgagctgagg ctgagcgtat cgctaaagag cagcgcattc aggccgagcg catgcgtgaa
    43801 gagaacatga ctgatgcaga gtggcagaaa gagctacagc gccgcgaaca ggtcaaagct
    43861 gaacgccgga cctatggtga gaacctccgc agtgctaccc attccgctgg tcgctcccgt
    43921 gccgctattg tcgctgacct tgagtcaggc ggtaactggc tggacagctt ataagacaca
    43981 cagtccctga cccgatgtta gggactcctc agttttgagg actcacagta tttaaactaa
    44041 tttaggagat tgattattat gacgattaaa gacgctttcc agtttggcat tgagccagta
    44101 cgcatcaccg ataccgacaa cattcaggtc aatgaaggtc tgccgactaa cgctgacccg
    44161 caggtctacg ccctccagtt agctaagacg gttaaggcta tgctgaacgg tgtacttaag
    44221 gacgctcaag agaacatccc gttccctgta gaagtcctgc cgacccgtaa cagcttaccg
    44281 accccaatca ttgcccacac tctgactgac cgctctgtgg tcgttcctgt ccgtggtggc
    44341 aagcgacctg aagtcgtgac cgctccgtct ggtactgaaa tcaccgtcga gccgattgag
    44401 caagctatcc tcgtgtccca ccagaccaag ttatgggacg ctaagtcatc caccggattc
    44461 actcagggca cactccagca ggacgctctg aacatctgcg ataacgtggt ccgtaccatc
    44521 aacgctcgca tggtcgacgt tctggaatcc tctaagctgc tgaagaccgt agagcttcct
    44581 gtcctgactg gtagcctgac cgctaaggca gacgctatca tggacgctct gtatgagaac
    44641 acagagtctt ctttcggttc tgaggtgtct gactacggta ttatcgctca tgagagccaa
    44701 ctgaaagcac tgtcccgtct ggctgctaag caaggcttct ccggtgaaga cgctatcgtc
    44761 gatatgctgg gaacagacat cgcctactac aacggtgaag acaagggtgt attcatgctg
    44821 gctaaacgct tcacggctct gagcttcggt tgcttccgtc acgatggcga gaatattacg
    44881 gtcgtccttt cacgtgatgg cgactctcag tcccacgact tggaaatcct cggtaaagtg
    44941 ttcgttgtcg ctgaagctgc tacgaccatc aagatgggca ctggctcagc tactgccgtc
    45001 ctgcctgtag tcaaacgcct gaagttcacc aagaccgaag cgtaagacca atgggtaaga
    45061 gcattagtaa aaggcttcag gtccatcgcc aagggcctga caggtggtgg tgggactcaa
    45121 ggtggggctt cggtcccgcc tcctgtagct cctcctgtag ctcctccaga tgacgctcag
    45181 caagctgtag tcgataccca aggtcaggag accgagggag gtaagaccat gagcagaggt
    45241 aagtctggac tgaatatcag caggacttca ggtggtggcc tgtcactgtg attaagacat
    45301 ctatacagcc tgtggagggt ggacgatatc aagcaacaat ctggatgaca cgcagagtct
    45361 ataagcggct atgctggtca ctggctgaag ctgaacagtg ggtgagacgt aaagtgccta
    45421 ccgttcaatc gttccaatca tcatgataac tatggtctgg accaatggtc tggacctttg
    45481 ttttgtacta gttatctagt gcagtagtac aatagacgaa ggaggcttta tgtccatgac
    45541 ttccgcagac ctaaggtccc tcctgactct ggtctataaa ctcgtgttcc tctctgtggg
    45601 tctctatatg gtcctctccg gtcgactcgg tgtgaatgta ttcgacactc tgtctaaggc
    45661 tgtgggtggg cttctggggg cttaggtttg gactcacagt gggaccgatg gtttctgaca
    45721 gaaaaatgtg aatgaccatc taatacatta agccagcaga cttcccccat agccccgcct
    45781 caagtccaga ccgaggccac cggggtcctg accagccgtc tagacatccc gtctaatgac
    45841 acaaggctga cacaaagcct gacacaaagt ccttgactca atgcaggacc tcaggcctac
    45901 taagggtccg cttcaagtcc ttgaagttcc agatgaactt aatgggcaca tctagtttat
    45961 ctttgccccg gacgccgtcg cggattgccg tcatttgcgg gccatgatcg atagcatctg
    46021 tccaactgaa gcaggagatt gacgttttga ccggattatc cagcaccggc aggcgttcga
    46081 tggtaatggt gtaggtcgat tcacgcgcac cactatttcc gccgctgatg ccgacattgc
    46141 ccgtcaaaat aggcaacata gcaatagcgc gtgccgtcag ctcgccgttt gcctgccgtt
    46201 gcggccccca gccctggcag atataagcgg gttttgctgt gccaatttca cgcgccagtt
    46261 tgatgatacg gtctaccggg atacctgtaa tttgcgaagc ccactgcggc gttttcgctg
    46321 ttttatcatc accttcacca agaatatagg ctttatagtg accatttttg ggtgcatctg
    46381 cgggtaaggt tttttcgtca tagccgacgc agtatttatc gagaaaaggt tgatcaacga
    46441 gattttcgtt aatcaatacc caggcaatac ccgcaaccag cgcggcatcg gtgcccgggc
    46501 gaataggaag ccattcatct tcacgaccag cagccgtatc ggtatatcgc ggatcgataa
    46561 caatcatttt tgcgttcgat ttctcgcgcg ctttttcaag aagataagtg atgccaccgc
    46621 cgctcatgcg ggtttcagcc gggttgttac caaacatcac gaccagcttg ctgttttcaa
    46681 tatccgtggt gctgttgcca tcattactgc cgtaggtgta gggcatggca caggaaattt
    46741 gcgcagtgct gtaggagcca tactggttta gtgaaccgcc gtagcagttc atcaggcgtt
    46801 tgaccgccga ggctgatgga gaagagcggg tcatattgcc gccaacaatc cccgaagagt
    46861 actgaatata tacagcctca ttgccatatt gttcgacggt ttttttcagg ctactggcga
    46921 tagtatccag ggcttcatcc cagctaatcc gttcgaattt gccttcgccg cgtttgccca
    46981 cgcgtttcat tgggtaattc aggcgatcgg gatgattaat acgccgacgg atggagcgac
    47041 cgcgcaaaca ggcgcgtacc tgatggttgc catacttgtc gctgccggtg ttgtcagttt
    47101 ccacccaggt cacttcatta tctttaacat gcagacgaag tgcacagcgg ctaccacagt
    47161 tgacggaaca ggcaccccag accacttttt cgctggcctg ttgtaccgct gctgctgcac
    47221 tgcgcagggt aaacggcaaa gaaaaaccgc ctgcagccag cgccagagaa cctatcgcag
    47281 tagatttaac gagtgttctg cggctgatgc ccaccatttg ttcatttttg gacataactc
    47341 actccctgtt ctttatcgtt atataaatgt ttatatattg aatatttagc gcgctaacaa
    47401 tagagggagt ctacccattt ggggttaata attattaatc catatcaata gaagggtatg
    47461 agtaataagg tggggttatg ttgaatgttc aaatcgccgg atgtgtcgca tccggcgttc
    47521 agtcgataat gtgttactgc ggttcggcag gcgcgccatc ctgggtagac tgcgcgggag
    47581 cagagacgtt accgctggtg gtacgggtgt agagaatttt atgcgtatca ttagcgcaat
    47641 gcccgacgac ctgggaatca ggctgatcaa cctggtcatt gggtacaata cttaacgtga
    47701 agctgctttc gggtacgcca ttattgataa tgcgctgtga tatatcgctc tgtatgcgct
    47761 cacaggatcc cggcgcggcg agtaccgtgg gtgaggcgag ggcgagcaga agcgcggcac
    47821 agtaggttga gtgtttcatc ataagctcct tacgcgaaga taacttcttt aagcatagca
    47881 tttaacgtgt aaagtactgt atttgctact atgattgaga atcatctcta ctctcaggtg
    47941 actgttgtga aatacaaatt actgccatgc ttactcgcga tattactcac aggatgtgac
    48001 cgcacagagg taacactttc atttaccccg gagatggcca gtttctctaa tgaattcgat
    48061 tttgatccgc tgcgtggtcc cgtaaaagat ttcactcaga cattaatgga tgagcaaggt
    48121 gaagtgacga aacgtgtttc ggggactttg tcggaagaag gctgttttga ttctctcgaa
    48181 ttactggatc tggaaaataa taccttggtt gccctggtac tggatgccaa ttattaccgt
    48241 gatgccgaga cgctggagaa aagagttcgt ttgcagggaa aatgccagct agcagaatta
    48301 ccttctgccg gggtgagttg ggaaaccgat gataatggtt tcgtgattaa agccagcagc
    48361 aaacagatgc agatggaata tcgctatgat gatcaggggt atccgctggg taaaaccacg
    48421 aaaagtaacg ataaaacatt atctgtcagc gccacgccat caacggaccc gatcaaaaaa
    48481 ttagattaca ctgctgttac tttactgaat aatcaacggg ttggtaatgt aaaacagagc
    48541 tgtgaatatg acaatcacgc caatccggtg gactgtcagc taatcattgt tgatgaagga
    48601 gtaaaacccg ccgtcgaacg ggtttatacc atcaaaaata cgatcgatta ttattaatac
    48661 tattgtgcgg tcggcttcag gagagtctga cccggtgttt tgtgctcagc cagatactga
    48721 tgctggaata tacacatgcg aatggcatta cgatattgac cattaataaa gaactcatgc
    48781 atcaattcac cttcaaccgt aaagccaagc ttgcggtaaa tgtgaatcgc tttttcattc
    48841 tctttatcaa cgatcagata cagcttatag agattgagaa cggtgaagcc atagtccatt
    48901 gctaatttgg cggcacgggt tgccagacct ttcccctgat actccgggga gataattatc
    48961 tgaaattctg cgcggcgatg aacatggtta atttccacca gctccaccag accggctttt
    49021 tcgccgtcac attccaccac aaagcgccgt tcgctctgat cgtgaatatg cttatcatac
    49081 agatcagaga gttcaacaaa ggcttcgtag ggttcctcaa accagtaacg catcacactg
    49141 gcgttattgt cgagttgatg tacatagcgt aaatcttcac gctccagcgg gcgtagctta
    49201 acactgtggg cgcttggcat aacgtgtcct tacattcctt aaatcaataa caggttaggg
    49261 gataataacg cggccagttc gacggtccag gcagcgcaaa gtatttggct cccagtaggc
    49321 attgatgttg gcgctttgct cacatttatc gcggttatca aaagcagcgt cggctttatc
    49381 ccactctttt tcagtgcgtt tattcacttt ctggcgcaga ttacgcgtgt cattccattg
    49441 ctctttttcc atagcggcgc gctggcggct ttgtgcactg tcgccagact caatcaccag
    49501 tttgttagtt tcggcatgaa cagttgtgct caataccagt gcgcaaggca gcagaaaagc
    49561 gagcaggccg attcgtttgc tgagagtgat tttcataatt cattccctgt atgaatgatt
    49621 aaagctgatt ctacaccatc cactgcgaac gcaaaacgtt caaggagggt gtttatattg
    49681 atgatattat gtcgccctat aactatacat gatgtcaata agtgacaaag atgattaaaa
    49741 caacgttact attttttgct actgcgctgt gtgaaattat tggatgcttt ctgccctggt
    49801 tgtggttaaa acgaaacgcc agtatctggc tgttgcttcc ggcggggatt tcactggcac
    49861 tgtttgtctg gttgttaacg ttgcatcctg cggcgagtgg gcgtgtttac gcggcttatg
    49921 gtggcgttta tgtttgcacg gcgttgatgt ggctgcgcgt tgtggatggc gtgaaactga
    49981 ctctttatga ctggacgggg gcgttaattg cgctttgcgg catgttgatc attgttgcgg
    50041 gctgggggcg cacgtaggaa cataaatcca ttttatcaat aagatacgag gaagtgtcag
    50101 ctgacaaaag gtattctatt tcatcttttg tcaaccattc acggcgcaaa tatacgcctt
    50161 tttttgtgat cactccggct ttttttgatc ttcatacttg tatggtagta gttcagttgc
    50221 gtagatttca tgcatcacga caagcgatgc aaggaatcga acatgaagat cgtaaaggct
    50281 gaagtttttg ttacctgtcc ggggcgtaat ttcgtcacat taaaaatcac cactgaggac
    50341 ggtattacgg gccttgggga tgccacccta aatggacgtg agctttccgt ggcctcttat
    50401 ttgcaggatc acctttgtcc gcagcttatt ggtcgcgatg cacaccgtat cgaagatatc
    50461 tggcagtttt tctataaagg tgcttactgg cgtcgcggtc cggttacgat gtcggccatt
    50521 tcagcggttg atatggcgct gtgggatatt aaagccaaag ctgccaacat gccgctttac
    50581 cagttactcg gcggcgcgtc tcgtgaaggg gtgatggttt attgccatac caccggtcac
    50641 agtattgatg aagctctgga tgattatgcc cgtcatcagg agctgggatt caaagccatc
    50701 cgcgtgcagt gcggaatccc tggtatgaaa accacctacg gcatgtcgaa aggtaaaggt
    50761 ctggcttatg aacccgcaac caaaggacag tggccggaag agcagctgtg gtcgacggag
    50821 aaatacctcg atttcatgcc gaaattgttt gacgcggtac gtaacaagtt tggatttaat
    50881 gaacatttgc tgcatgacat gcaccatcgc ttaacgccta ttgaagcggc gcgctttggt
    50941 aaaagcattg aagattatcg catgttctgg atggaagacc cgacgcctgc ggaaaaccag
    51001 gaatgcttcc gtctcattcg ccaacatacc gtcacaccca tcgcggtggg tgaagtcttc
    51061 aacagcatct gggactgcaa acaactgatt gaagagcaac tcatcgatta tatccgcacc
    51121 acgctgactc atgcaggcgg aattaccggt atgcgtcgga ttgccgattt tgcttcgctg
    51181 tatcaggtac gtactggctc acacggtcct tccgatttgt caccagtctg catggctgcg
    51241 gcgctgcact ttgatctgtg ggtccccaat ttcggtgtcc aggaatacat gggttattcc
    51301 gaacaaatgc tcgaagtctt cccgcacaac tggactttcg ataacggcta tatgcatccg
    51361 ggagaaaaac cgggtcttgg catcgaattc gatgaaaagc tggcggcgaa atatccctat
    51421 gaacctgctt atctgccagt cgcacgtctg gaagatggca cgctgtggaa ctggtaagga
    51481 gtaaggtaat gaaaagcatt ttaattgaaa aaccgaatca actgtcgatt atcgaacgtg
    51541 aaatacccac cccgtcagcg ggtgaagtac gagtaaaagt gaaacttgcc ggaatttgtg
    51601 gttcagatag ccatatttac cgtgggcata atccttttgc gaaatatccg cgcgtcattg
    51661 gtcatgaatt ctttggcgtc attgatgcag tgggtgaagg cgtggaaagc gccagagtcg
    51721 gtgaacgcgt tgctgtcgat ccggtggtca gctgtgggca ttgctatccg tgctctatag
    51781 gtaagccgaa cgtttgtacg acacttgctg tattaggtgt gcacgctgac ggtggtttca
    51841 gtgaatatgc cgtggttccg gcaaaaaatg cgtggaaaat tcctgaagca gtggccgatc
    51901 aatatgcggt gatgatcgaa ccttttacca ttgcggctaa cgttaccggt catggtcaac
    51961 cgactgaaaa tgataccgtt ctggtttacg gtgccggtcc aatcggcctg acgatcgttc
    52021 aggtattaaa aggcgtctat aacgttaaaa atgtgattgt tgccgatcgc attgatgaac
    52081 gactggaaaa agcgaaagag agcggggcag actgggcgat taataacagc cagacaccac
    52141 ttggcgagat tttcgatgaa aaaggcatcc aaccgacatt agttatcgat gccgcttgtc
    52201 atccttcaat cttgaaagag gccgtaacgc tggcttctcc agcggcacgt attgtattga
    52261 tgggcttctc cagtgaaccg tctgaagtga ttcagcaagg aattaccgga aaagaactct
    52321 ctattttctc ttcacgctta aatgcaaata aattcccggt cgttatcgac tggttaagta
    52381 aagggttaat taaaccagaa aaactaatta cccatacgtt tgatttccag catgttgcgg
    52441 atgccattag tttatttgaa caggatcaaa aacattgctg caaagtctta ctcacttttt
    52501 ctgaataata ccaataacgg cgaataagta gtacgcatct tacctctttt ttagagataa
    52561 ccattatgac aatagaaaaa cacgaaagaa gcactaagga tttggtgaaa gcagcagtat
    52621 cgggatggct gggcactgcg cttgaattta tggatttcca gttatattcg ctcggcgcag
    52681 cgttagtgtt tcatgaaata ttttttcctg aatcatcaac ggcaatggcg ttaattctgg
    52741 caatgggaac ctatggtgca ggttatgtgg cacgtattgt cggagcattt attttcggca
    52801 aaatgggcga cagaataggg cgtaaaaaag tgctctttat taccatcacc atgatgggga
    52861 tctgtaccac cttaattggt gtgttaccga cttatgcaca aattggtgtt tttgccccca
    52921 tcttgctggt gacgctgcgt attattcagg ggttgggtgc aggtgcggaa atttccggtg
    52981 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
    53041 tggctatggg aactaactgc ggaaccttga gcgcaacggc aatctgggcc tttatgttct
    53101 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tatacctttc ctggcgagcg
    53161 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
    53221 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
    53281 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggctggt aactcaggtt
    53341 taattcagac tttccttgca ggctatttag tgcagacgtt attgtttaac aaagcaattc
    53401 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
    53461 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
    53521 tgctggcatg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
    53581 tggttgcact gattgtgatt cataactgtg cggtgcttgg attatttgct ctggaaaaca
    53641 ttaccatggc agaaatgttc ggctgtaaaa accgctttac ccgaatggct atttctaaag
    53701 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcggggatt ttctgcacca
    53761 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattctgtg attggtttaa
    53821 tctctgcgct gaaaatgcca gaagtgaaag accgtgattt aagtgcgctg caagacgccg
    53881 cagaagatca accacatgtt gtaagagctg cgcaaccgtc cagaagtctg taaatcctca
    53941 atcccttctc ttaccggaga ggggattttt attcatataa aaaaacatgt agcttgctat
    54001 atttaatttt aaggaattgt tatgggaaat catttgttat cagcaaaagc gacgctccct
    54061 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
    54121 cgtgcgcatc agggcgtgta tgccgatatt cttgctacgg aacatttcag tgactgggga
    54181 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
    54241 aatctttata ccgttgcgga aatgtcggct gatgcgtgga cggctcgcgt cgttggcgtc
    54301 gttaaaaaag ccttacacgt acaaatagac ggcttagaaa ccgtgttggc tgcgatgtgt
    54361 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
    54421 gcgaccggac agttaatgct cgatcacccg atggtcgttg ccgacgtgca aaatccccac
    54481 cagccgaaaa ctgcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
    54541 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
    54601 gacgttgtca cttcctatgc gcaagccatt gatgtaaaac tggcacaatg gatcgaagat
    54661 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
    54721 ctggcgaaaa tcgaacaact taccggtgtg cgcgatccgg caggcgttgc ctgtgaacct
    54781 ttccgccagt gggtaataga agataacttt gttgccggac gtccggaatg ggaaaaagcg
    54841 ggagtcgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaat
    54901 ggcagtcatt cattcctggc gtatctgggc tatcttgccg gatatcagca cattaatgac
    54961 tgcatggagg atgaacatta tcgtcatgcg gcgtatacct taatgttgca ggaacaagcg
    55021 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgaacgc
    55081 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
    55141 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
    55201 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
    55261 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggcagt acaaagtagt
    55321 gctgaaggga cagcgcgcgt gcagtcattg ttggcgatta aggcgatatt tggtgatgat
    55381 ttgccagata atagcttgtt tacgactaaa gtgacggaag cgtacttgtc tttattagcg
    55441 catggcgcga aagcgacagt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
    55501 cggcggcatt cacacgctta gttcatgccg agacgaatca ggtccagtgg tgccagtttc
    55561 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
    55621 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
    55681 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
    55741 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
    55801 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccata agtcgggggg
    55861 aaagactctc accccggatg ggatgagagg aaatgaatta ctcttcgcta aaccagtcgc
    55921 tgttttcctg gcggatctgg cgcacggatt cgctgatttc ctgcaaatgt tgcgtcattg
    55981 cacgttctac cgcatcgcca tcacgttttt gcagggcaga gaaaatatca agatgctggc
    56041 gtaacagcat ttctggtgga gaaacgtggt cgaagctcat atagcgcacg cgatcaacgg
    56101 tcgctttcag gttctcaatg gtatcccacg ccagttgaca gtcggcaatc tgcgtcagga
    56161 gttgatggaa gttgtcatca agttcaaaaa aatcatccag ttgcttgcgc tcaatggcaa
    56221 tgcgttgctg gtgaagattt tgttccagtt ggtagcactg gctttcggta atcatgctcg
    56281 ccgcccgacg cgccaccgcg cactcaatgg cctgacggat aaaactgccg ttgcgcacct
    56341 gggccatgga aattttgttg acgtagctgc cacgctgcgg acgaatttga atcagaccgt
    56401 tttccgccag tttaataaag gcttcacgaa ccggctggcg tgacacattg aaacgaacag
    56461 aaacttcttt ttccgacaac ggtgtgcctg gtgcaatcag gcaatggaca atgtcgcggc
    56521 gaagaatacg ataaatctgc tgattgacag gctgtgtggg attaagttgc gtttcgacgg
    56581 tcattcgttc ttgcttgtga gtgagttaac tgcatgagtc taccactttt ttgtgacaag
    56641 caataacccg gcagttacgc cgggtataaa aattactgac ggtggacatt cagtccggca
    56701 tagctttggg taaccggcat catttccagt gtattgatat tgacgtgagc aggcagcgtt
    56761 gacacccacc agacggcttc gctgacatct tctggcgtca atgcaacggt attttgatag
    56821 gttttttccg ctttaccgtc atcgccttta aagcggacat tggaaaactc ggtgccaccc
    56881 accagacccg gttcgatgtc ggtgacgcgc accgccgtac catgcaggtc cgtacgcaga
    56941 ttcaggctaa attgacgaac aaacgctttc gtcgcaccgt aaacgttacc accggcatac
    57001 ggccagctac ctgccgttga gccaatgtta ataatatgac catgattacg ttcaaccata
    57061 cccggtaaga cggcgcgcgt catatatacc aggcctttgt tgttggtatc aatcatcgtt
    57121 tcccagtctt caatgctggc tttatgagca ggctccatgc ccaacgccag gccggcattg
    57181 tttaccagga tatcaatatt gctccactcg gcagggagcg atgccagcat ctcttcaata
    57241 gcggcgcggt tgcgaacgtc cagttgggcg atatacagat tatctcccag ttcgtctttt
    57301 aactcctgca accgctcctg gcgacgacca gtggcgataa ctttatgccc ttgttgaata
    57361 aaacgacgag taatgcattc accaaaacct gccgttgctc cagttactaa aacgatcatc
    57421 tcactgttcc tcaacgattt tgtgtgtcaa taacatagca cgtgatgctt aagagcgggt
    57481 aatactggtt tgcaagattg cactccgtta tcagtaagcc tacgctatgg aacacctcgc
    57541 gttcaggaga agaaaatgac aacaatgaat cctttccttg tgcaaagcac actgccgtat
    57601 ctggctcccc attttgatca aattgccaat catcactatc gcccggcatt cgatgaggga
    57661 atgcagcaaa agcgggcaga aattgctgcc atcgcgctta acccgcaaac acctgatttc
    57721 aacaatacta ttctggcact ggaacaaagc ggagaattac ttacccgcgt taccagcgtc
    57781 ttttttgcga tgactgcggc gcataccaat gatgaattac agcgtcttga tgaacagttt
    57841 tccgctgaac tggcggaact tgcgaatgat atctatctga acggggaatt attcgcgcgg
    57901 gtagatgctg tctggcaacg ccgtgaatcc ctggggcttg atagtgaatc catccgcctg
    57961 gtggaggtga ttcatcaacg ttttgtcctt gccggagcca aacttgagca agctgataaa
    58021 gcaaaattaa aagtactgaa tacagaagct gcgaccctga ccagccagtt taaccagcgg
    58081 ttactggcag caaataaatc cggcggtctg gttgtgaacg atatcgcgca gctggcagga
    58141 atgagtgaac aagagattgc gctggcggca gaggcggctc gcgagaaagg tctggataac
    58201 aactggctga ttccgctgct gaataccacc cagcaaccag cgcttgctga actgcgcgat
    58261 cgcgcgacgc gtgaaaaact gtttactgcg ggctggaggc gagcggaaaa aaatgatgcc
    58321 aatgataccc gcgctatcat tcaacgtctg gtggagatcc gcgcgcagca ggcgaaactg
    58381 cttggttttc ctcattatgc cgcatggaaa atcgccgatc agatggcaaa aacgccagaa
    58441 gcagcgctca actttatgcg ggaaattgtt ccagcggcgc gtcaacgtgc gagcgatgaa
    58501 ttagcctcca tacaggcggt tatcgataag cagcagggcg ggtttagcgc gcagccgtgg
    58561 gactgggcat tttatgccga gcaggtaaga cgtgagaaat tcgatctcga tgaagcgcag
    58621 ctcaagccat attttgaatt aaacacggtg ttgaatgaag gcgtattctg gactgcgaat
    58681 cagctcttcg gtattaagtt tgtcgaacgt tttgatattc ctgtctacca tcctgacgtt
    58741 cgtgtgtggg aaatttttga tcataatggc gtggggctgg cgttatttta cggtgatttc
    58801 ttcgcccgtg attcaaaaag cggcggcgca tggatgggca attttgttga gcaatcaacg
    58861 ctgaatgaaa cgcacccggt aatttataac gtctgcaatt atcagaaacc cgctgccggt
    58921 gagcctgcgt tgttactctg ggatgatgta ataaccttat tccatgaatt tggtcatacg
    58981 ctgcacggcc tttttgcccg ccagcgttat gccacgcttt ccggcaccaa cacgccgcgt
    59041 gattttgtcg aatttccgtc gcaaatcaac gaacactggg caacacatcc gcaggtattc
    59101 gctcgctacg cccggcatta tcagagcggg accgcaatgc ctgacgaact tcaacagaaa
    59161 atgcgtaatg ccagcctgtt caataaaggg tatgagatga gcgaactgct tagcgccgca
    59221 cttctcgata tgcgctggca ttgcctggaa gaaaacgaag caatgcagga tgtcgatgat
    59281 ttcgaattgc gggcgctggt ggcggaaaat atggatcttc ctgctatacc gccacgctat
    59341 cgcagcagtt atttcgccca tatttttggt ggcggatatg ccgcaggtta ttacgcttat
    59401 ctgtggacgc aaatgttggc cgatgatggt taccagtggt ttgttgagca gggcggatta
    59461 acgcgtgaaa atgggcggcg ttttcgcgag gcgattcttt ccagaggtaa cagcgaagat
    59521 ctggaacgcc tgtaccgaca atggcgcggt aaggcacctc agattatgcc gatgttgcaa
    59581 catcgtggct tgaacgtata aacaccacca atggctggcc tggatttatc gccagaccag
    59641 cctcgttata gcagtaattg cagccgctgg gcgagggcaa acttcaccgc taatgcgaat
    59701 gccgtttcgc agtcgccagc aaaattactt tttttgtata ctcttccgtt ataataattt
    59761 tcaaattcag ccggaccaga tgatcaacat tagcggcagt ctggataaga aaagcgcgcc
    59821 ggccgtcgtt cgggtcactc atttgcagaa ataaaaataa attagcctga tggcctgaac
    59881 tactgggcca ttagggtatt gggttatgct atttgtcatc cttttaattg cgataatgca
    59941 gagttaattt atagaattaa acactttttt atctgctg
//
LOCUS       DAJOTB010000011        59823 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJOTB010000011 DAJOTB010000000
VERSION     DAJOTB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.011"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..467
                     /locus_tag="OHN28_000702"
     CDS             <1..467
                     /locus_tag="OHN28_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2296144.1"
                     /translation="KIVADTCEINVNESASTTGTVTFADTYPADYSGDGSIGTSKDFK
                     IELIKCDPLVDKLNLTFSGTTTDAGFLRLENSEAGAGSATNVGITVTNKNGGANDVKF
                     DGSVPDASTDVDNAGSITPTIFNYTANVIQVGNNAPTAGKYASSATFEVFYR"
     gene            513..1229
                     /locus_tag="OHN28_000703"
     CDS             513..1229
                     /locus_tag="OHN28_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2296145.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKLEENRQTLLHIVFTGDKNKLPQDRE
                     SLFLANIKSVSAMPEELKDRNTLQFAMKARLKLFWRPASLDNSDALTAWEKLKFHKEA
                     GKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPFSEQRFSLPVGAGSI
                     VEWSVITDYGSSSGKRQQKL"
     gene            1296..3773
                     /locus_tag="OHN28_000704"
     CDS             1296..3773
                     /locus_tag="OHN28_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2296146.1"
                     /translation="MLLPLHRTFVLTGITFALSAVYSLSYARDEFNLRILELDSPLEN
                     TQVLEDFVNNNNLTPGVYLTSVMWGQEYLDKRNITFILSSDKKRLIPRFTKADLREFG
                     LKVDDIPALQVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISP
                     KYWSDGESAVWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWE
                     SISTSLQRDIKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVR
                     GIANSDAQVVIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQ
                     ASSTVPILQREGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGAS
                     IYHSGVTGIGADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVT
                     LASYRYSTSQFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYL
                     NGYQQDYWGMHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSR
                     WLPDSWAMYNVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRY
                     RSSVGEFGLGYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKV
                     QNAQGVYTDYWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDF
                     DARSGIRALLTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKE
                     INQQCTGVVTVPEKYTTGIYKATMDCR"
     gene            3795..4805
                     /locus_tag="OHN28_000705"
     CDS             3795..4805
                     /locus_tag="OHN28_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2296147.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFKIQIEKCSQDVKVDYRFTSSGSTGVTGNNILNIESGSGMAEGV
                     GLQILDSNNNVMVFDTDYTPVQKTTLNQNVTIPLKARYIKMGSVKSGRVNSVATFEVY
                     YR"
     gene            5025..7274
                     /gene="fhuA"
                     /locus_tag="OHN28_000706"
     CDS             5025..7274
                     /gene="fhuA"
                     /locus_tag="OHN28_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCQ2296148.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            7326..8123
                     /gene="fhuC"
                     /locus_tag="OHN28_000707"
     CDS             7326..8123
                     /gene="fhuC"
                     /locus_tag="OHN28_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCQ2296149.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            8123..9013
                     /gene="fhuD"
                     /locus_tag="OHN28_000708"
     CDS             8123..9013
                     /gene="fhuD"
                     /locus_tag="OHN28_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCQ2296150.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            9010..10992
                     /gene="fhuB"
                     /locus_tag="OHN28_000709"
     CDS             9010..10992
                     /gene="fhuB"
                     /locus_tag="OHN28_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCQ2296151.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWLP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(11027..12307)
                     /gene="hemL"
                     /locus_tag="OHN28_000710"
     CDS             complement(11027..12307)
                     /gene="hemL"
                     /locus_tag="OHN28_000710"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCQ2296152.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLNELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            12532..13953
                     /gene="clcA"
                     /locus_tag="OHN28_000711"
     CDS             12532..13953
                     /gene="clcA"
                     /locus_tag="OHN28_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCQ2296153.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            13977..14042
                     /locus_tag="OHN28_000712"
     CDS             13977..14042
                     /locus_tag="OHN28_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296154.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            14035..14379
                     /gene="erpA"
                     /locus_tag="OHN28_000713"
     CDS             14035..14379
                     /gene="erpA"
                     /locus_tag="OHN28_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCQ2296155.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(14426..15049)
                     /locus_tag="OHN28_000714"
     CDS             complement(14426..15049)
                     /locus_tag="OHN28_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCQ2296156.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(15087..15887)
                     /gene="btuF"
                     /locus_tag="OHN28_000715"
     CDS             complement(15087..15887)
                     /gene="btuF"
                     /locus_tag="OHN28_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCQ2296157.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(15880..16578)
                     /gene="mtnN"
                     /locus_tag="OHN28_000716"
     CDS             complement(15880..16578)
                     /gene="mtnN"
                     /locus_tag="OHN28_000716"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCQ2296158.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            16662..18179
                     /gene="dgt"
                     /locus_tag="OHN28_000717"
     CDS             16662..18179
                     /gene="dgt"
                     /locus_tag="OHN28_000717"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCQ2296159.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            18309..19733
                     /gene="degP"
                     /locus_tag="OHN28_000718"
     CDS             18309..19733
                     /gene="degP"
                     /locus_tag="OHN28_000718"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCQ2296160.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            19888..21045
                     /gene="cdaR"
                     /locus_tag="OHN28_000719"
     CDS             19888..21045
                     /gene="cdaR"
                     /locus_tag="OHN28_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCQ2296161.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(21099..21485)
                     /locus_tag="OHN28_000720"
     CDS             complement(21099..21485)
                     /locus_tag="OHN28_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCQ2296162.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(21795..22619)
                     /gene="dapD"
                     /locus_tag="OHN28_000721"
     CDS             complement(21795..22619)
                     /gene="dapD"
                     /locus_tag="OHN28_000721"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCQ2296163.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(22650..25322)
                     /gene="glnD"
                     /locus_tag="OHN28_000722"
     CDS             complement(22650..25322)
                     /gene="glnD"
                     /locus_tag="OHN28_000722"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCQ2296164.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(25384..26178)
                     /gene="map"
                     /locus_tag="OHN28_000723"
     CDS             complement(25384..26178)
                     /gene="map"
                     /locus_tag="OHN28_000723"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCQ2296165.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            26546..27271
                     /gene="rpsB"
                     /locus_tag="OHN28_000724"
     CDS             26546..27271
                     /gene="rpsB"
                     /locus_tag="OHN28_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCQ2296166.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            27406..28257
                     /gene="tsf"
                     /locus_tag="OHN28_000725"
     CDS             27406..28257
                     /gene="tsf"
                     /locus_tag="OHN28_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCQ2296167.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            28404..29129
                     /gene="pyrH"
                     /locus_tag="OHN28_000726"
     CDS             28404..29129
                     /gene="pyrH"
                     /locus_tag="OHN28_000726"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCQ2296168.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            29279..29836
                     /gene="frr"
                     /locus_tag="OHN28_000727"
     CDS             29279..29836
                     /gene="frr"
                     /locus_tag="OHN28_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCQ2296169.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            29928..31124
                     /gene="ispC"
                     /locus_tag="OHN28_000728"
     CDS             29928..31124
                     /gene="ispC"
                     /locus_tag="OHN28_000728"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCQ2296170.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            31313..32071
                     /gene="ispU"
                     /locus_tag="OHN28_000729"
                     /gene_synonym="uppS"
     CDS             31313..32071
                     /gene="ispU"
                     /locus_tag="OHN28_000729"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCQ2296171.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            32084..32941
                     /gene="cdsA"
                     /locus_tag="OHN28_000730"
     CDS             32084..32941
                     /gene="cdsA"
                     /locus_tag="OHN28_000730"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCQ2296172.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            32953..34305
                     /gene="rseP"
                     /locus_tag="OHN28_000731"
     CDS             32953..34305
                     /gene="rseP"
                     /locus_tag="OHN28_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCQ2296173.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            34335..36767
                     /gene="bamA"
                     /locus_tag="OHN28_000732"
     CDS             34335..36767
                     /gene="bamA"
                     /locus_tag="OHN28_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCQ2296174.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            36889..37374
                     /gene="skp"
                     /locus_tag="OHN28_000733"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             36889..37374
                     /gene="skp"
                     /locus_tag="OHN28_000733"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCQ2296175.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            37378..38403
                     /gene="lpxD"
                     /locus_tag="OHN28_000734"
     CDS             37378..38403
                     /gene="lpxD"
                     /locus_tag="OHN28_000734"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCQ2296176.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            38508..38963
                     /gene="fabZ"
                     /locus_tag="OHN28_000735"
     CDS             38508..38963
                     /gene="fabZ"
                     /locus_tag="OHN28_000735"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCQ2296177.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            38967..39755
                     /gene="lpxA"
                     /locus_tag="OHN28_000736"
     CDS             38967..39755
                     /gene="lpxA"
                     /locus_tag="OHN28_000736"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCQ2296178.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            39755..40903
                     /gene="lpxB"
                     /locus_tag="OHN28_000737"
     CDS             39755..40903
                     /gene="lpxB"
                     /locus_tag="OHN28_000737"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCQ2296179.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            40900..41496
                     /gene="rnhB"
                     /locus_tag="OHN28_000738"
     CDS             40900..41496
                     /gene="rnhB"
                     /locus_tag="OHN28_000738"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCQ2296180.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            41533..45015
                     /gene="dnaE"
                     /locus_tag="OHN28_000739"
     CDS             41533..45015
                     /gene="dnaE"
                     /locus_tag="OHN28_000739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCQ2296181.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            45028..45987
                     /gene="accA"
                     /locus_tag="OHN28_000740"
     CDS             45028..45987
                     /gene="accA"
                     /locus_tag="OHN28_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCQ2296182.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            46085..48226
                     /gene="ldcC"
                     /locus_tag="OHN28_000741"
     CDS             46085..48226
                     /gene="ldcC"
                     /locus_tag="OHN28_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCQ2296183.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            48283..48672
                     /locus_tag="OHN28_000742"
     CDS             48283..48672
                     /locus_tag="OHN28_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2296184.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            48737..50050
                     /gene="tilS"
                     /locus_tag="OHN28_000743"
     CDS             48737..50050
                     /gene="tilS"
                     /locus_tag="OHN28_000743"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCQ2296185.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(50084..50344)
                     /gene="rof"
                     /locus_tag="OHN28_000744"
     CDS             complement(50084..50344)
                     /gene="rof"
                     /locus_tag="OHN28_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCQ2296186.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(50331..50531)
                     /locus_tag="OHN28_000745"
     CDS             complement(50331..50531)
                     /locus_tag="OHN28_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCQ2296187.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            50697..51242
                     /locus_tag="OHN28_000746"
     CDS             50697..51242
                     /locus_tag="OHN28_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCQ2296188.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            51239..51661
                     /gene="arfB"
                     /locus_tag="OHN28_000747"
     CDS             51239..51661
                     /gene="arfB"
                     /locus_tag="OHN28_000747"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2296189.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            51675..52385
                     /gene="nlpE"
                     /locus_tag="OHN28_000748"
                     /gene_synonym="cutF"
     CDS             51675..52385
                     /gene="nlpE"
                     /locus_tag="OHN28_000748"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCQ2296190.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(52541..53365)
                     /locus_tag="OHN28_000749"
     CDS             complement(52541..53365)
                     /locus_tag="OHN28_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCQ2296191.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(53418..55136)
                     /gene="proS"
                     /locus_tag="OHN28_000750"
     CDS             complement(53418..55136)
                     /gene="proS"
                     /locus_tag="OHN28_000750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCQ2296192.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(55247..55954)
                     /gene="tsaA"
                     /locus_tag="OHN28_000751"
     CDS             complement(55247..55954)
                     /gene="tsaA"
                     /locus_tag="OHN28_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCQ2296193.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(55951..56355)
                     /gene="rcsF"
                     /locus_tag="OHN28_000752"
     CDS             complement(55951..56355)
                     /gene="rcsF"
                     /locus_tag="OHN28_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCQ2296194.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(56473..57288)
                     /gene="metQ"
                     /locus_tag="OHN28_000753"
     CDS             complement(56473..57288)
                     /gene="metQ"
                     /locus_tag="OHN28_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCQ2296195.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(57328..57981)
                     /gene="metI"
                     /locus_tag="OHN28_000754"
     CDS             complement(57328..57981)
                     /gene="metI"
                     /locus_tag="OHN28_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCQ2296196.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(57974..59005)
                     /gene="metN"
                     /locus_tag="OHN28_000755"
     CDS             complement(57974..59005)
                     /gene="metN"
                     /locus_tag="OHN28_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCQ2296197.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            59193..59765
                     /gene="gmhB"
                     /locus_tag="OHN28_000756"
     CDS             59193..59765
                     /gene="gmhB"
                     /locus_tag="OHN28_000756"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCQ2296198.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        14424 a        14438 c        16210 g        14751 t
ORIGIN      
        1 gtaaaattgt tgccgatact tgcgaaatta atgtaaacga gtctgccagt acaacaggga
       61 cagtcacttt tgcagatact tacccagcgg actattctgg agacggtagt attggaacgt
      121 caaaagattt taaaattgaa ttaataaaat gcgatccgtt ggtggacaaa ctaaacctga
      181 cattttctgg aacgacaacg gatgcaggtt tcctgcgtct ggaaaatagc gaagctgggg
      241 ctggcagtgc aacaaatgtg ggtattacgg tgacaaataa aaatggcggt gctaacgatg
      301 tgaaatttga tggttcagtt cccgatgcat ccactgatgt tgataatgcc gggagcatta
      361 ctccaacaat attcaattac accgccaatg ttattcaggt tggtaataac gcgccgactg
      421 cgggtaagta tgcctcatct gcgacgttcg aagtatttta tcgctaatat tcatagtgtt
      481 tgaaaggact gtttgaccac ggagggttat ttatgtcagt tttttctcga gtttgtttag
      541 gttttacgtt tcttcttacg gcattgcaag ttcaggcaag tattgttctt ggcggtacac
      601 gtattatcta tccttcaaat caaaatgaag ttcaaatcac attaaaaaat aaagatgcat
      661 atgcacgcta tttggtgcaa agctgggtga gtaatattga tggctctaaa gccccctttc
      721 tcattacgcc tccagtttat aaacttgaag aaaatcggca aacattactc catattgtat
      781 ttacaggtga taaaaacaaa cttcctcagg atagggaaag tttgtttctg gcaaacataa
      841 aatctgtttc tgcgatgcca gaagaactaa aagatagaaa taccttgcaa tttgcaatga
      901 aagcccggct taaattattt tggcggccag cttctttgga taatagtgat gctctcactg
      961 catgggaaaa acttaagttc cataaagaag cggggaagct catagtaaaa aatccaacgc
     1021 ctttttatat ttcattcagt gatctgacag tatcgggaaa aaatattgtt cccaccgaga
     1081 gcaaatctga acctggcgca ttattgatga tggtaggacc attttctgag cagcggttta
     1141 gtcttcctgt cggagcggga agtattgttg aatggtcggt gataactgac tacggttcaa
     1201 gttctgggaa acgacaacaa aagttataaa tcaccgaagt gtattactat aaataaccga
     1261 tctgtattta taatattacg ggcgcaaggg aatttatgct gctaccattg catcgcacat
     1321 ttgtcttaac tgggataaca tttgcattat cggcagtgta ttcattgagc tatgcacgag
     1381 atgaatttaa ccttcgtatc ctggagctgg atagcccact tgaaaataca caagtgctgg
     1441 aggattttgt taataataat aacttaactc cgggagtata tcttacctct gttatgtggg
     1501 ggcaagagta tcttgataag agaaatatta ccttcattct gtcttcggat aaaaaaaggc
     1561 tcatccctcg ttttacaaag gcggatctcc gtgagttcgg tcttaaagtt gacgacattc
     1621 ccgcgttaca agtaatggat gatgacactg agtttggtga tattgctcag attattgatg
     1681 gcgcacgtta tgatttccag ttagacagcc aaacgctgtg tctgcggatc ccgcaaatct
     1741 atcagaatgc gcgggccgca gggagtattt caccgaaata ctggagtgat ggtgagagtg
     1801 cggtttggtt aagttattac gccagtggtt cccggcaaaa cagtgatggc gataacctga
     1861 attcaaattg gttgaattta aattcaggga ttaatttagg cgtatggcgg ctacgaaata
     1921 ataccgttta cagtgactcc agttgggagt cgatcagcac cagtttgcaa cgcgatatca
     1981 aagcattaag aagccagatg gaagtcgggc agacgttcac aaatggcgat ctttttgaca
     2041 gtgtgcagat gacaggtata aagcttgaaa cggacaccag tatgctgcct gacagcgaac
     2101 aaggctttgc gccagtggtt cgtggtattg ccaacagcga tgcacaagtt gtgattaaac
     2161 aaaacggcta tgttatttac caaacgtggg tatcggcagg gccatttgaa attaaagact
     2221 taagccaggt aacggcgggg gccgatctgg aagtcaccat caaagaaacc aacgggcaag
     2281 aacacagttt tattcaggcc agttcaacgg ttccaattct acagcgagaa ggcgcattaa
     2341 aatatagtct tgcaactgga aaataccgtg acaacgataa tcatgctgag acacctgttt
     2401 ttggtgttgc aaccgcgata tatggcttgc cctatggaat aacgatttat ggcggcatac
     2461 ttggtgcctc aatttatcat tctggtgtga cagggattgg cgctgattta ggccgtctgg
     2521 ggtcggtatc tgttgatatc accgcagcag aaacgaagtt tgatgatggg cgtgatgatg
     2581 ctacaggact gtcatggcgt gcacagtatg cgaaagattt tccagacact gatacgacag
     2641 taactctggc aagttatcgt tattctacat cacaatttta cactttccag gaagcacttg
     2701 atcaacgtga tacacctgat gacaagggta tttacagtta tcgacagaca aataatcggc
     2761 gtaatcgttt acaaattaat ctttcacaaa atattggtcg ttggggctcg gtctacctta
     2821 acggttatca acaggattat tggggaatgc atggggccga acgcagtata ggaatgggat
     2881 acagtacgac ctggagtaac attaattggt ctgttaatta cacactaacg aaaacgccag
     2941 gaatggctgg tgagcagcaa ttttcattaa cactcaatat tcctctctct cgctggctgc
     3001 cagattcatg ggcgatgtat aacgtaaacc gttccgataa aagtaataca tcacatcaat
     3061 tgggaattgg cggtactgct ctgcaagata acaacctgag ttataattta cagcaaagtt
     3121 atactgataa taatgtgggt tatgatgcca gcatgaatgg acgttatcgt agctcagtgg
     3181 gggaattcgg tctgggatat agctatgata aaaatagtcg tcagtggaat tacagtgcgc
     3241 aaggtgcggt ggttgctcat gctcatggag taacattggg gcaatcagtg caggattcat
     3301 tcgcgatcgt gcatattaat gagggtgcca atgtaaaagt acaaaatgcg caaggcgttt
     3361 atactgatta ttggggaaac gcgattgttc ccaatatgac gaattatcga cataacgcta
     3421 ttactgtgaa tacacagggg cacgatagtc ttgatatttc tgatgccaca caagatgtta
     3481 ttccttcgaa aggggctgtt gttggtgtcg attttgatgc acgtagcgga atacgagcct
     3541 tgttgacatt agtccataat aaagaacgcg taccctttgg ggcattgctg acattaggga
     3601 atagtacagc gattgttggt gaagatgggg aggtttatat tactggtgtg caagaatcaa
     3661 tgacatttac ggtacagtgg ggaaaagaga ttaaccagca atgtacagga gttgtcacag
     3721 tgccagaaaa atacaccacg ggtatctata aagccacaat ggattgccga taacaaataa
     3781 gacaggaaaa aataatgaag ccaggattcg tctcttatat acgaacatta ttgaccataa
     3841 tatttcttag ctggggcgtc attcctgagg ttttcgccgg ttgtaacatt tctggaaatg
     3901 gaggaaatcc ggcaacagta caattctccg tgcctgcgtt ggttgttaat agcgatgccg
     3961 ttcctggaac gattattgcg acagtaaatg cgaacagtcc gtcaatttca gtttcctgta
     4021 cggcaaatgg tgagatctgg caaggatata ccgtattgac aaacggtgat atgcgtactg
     4081 ataacccact taacaatgtg tttcagacta atgtgccagg aatcggtttt cgcgcggcgt
     4141 gggcgaataa caccagtgcg caactcaccg ccggatattt aatgacgccc tggcataaag
     4201 gctcgtcaac agtgattaaa ggatatggtt atccgattac aatacatgca gctattcagt
     4261 ttattgtcac aggacctgtc ggtaatggga ccattgatac cagcaaactg gttgctgact
     4321 ggaagtatga caatcgtgtt attggagagc ttcgttttag ttctgttccg gtcaatattc
     4381 aaccgggcac atgcgacctt atagaaaaaa atattaccgt tccactcaat gatattggca
     4441 cagatgattt tgatgttaac aatgtgtcac ctgtagtttc cgatgatcgg tttaaaattc
     4501 agattgaaaa atgttctcag gatgtaaaag ttgattaccg ctttacttca tcaggttcta
     4561 ctggagtaac gggtaataat attcttaata ttgagtctgg ttcaggaatg gcggaaggag
     4621 ttggtctgca gatcctcgat agtaataaca atgtgatggt atttgataca gactatacac
     4681 ctgttcaaaa aacgacgtta aatcaaaatg ttactattcc gttaaaagca cgctacatca
     4741 aaatgggtag cgtcaaaagt ggtcgggtta attctgttgc caccttcgaa gtgtattacc
     4801 gataagcttt tccctttgcc ataaatgtta atttttggta atggaatttt tatggtttac
     4861 ggtgcaaaga ttaacatttt tgattcatct gattgattct cgccccttca tggacgcaca
     4921 gagcgtatat cacttgcgaa cccgccagcg tttcgaatat tatcgcatct ttataataat
     4981 cattctcgtt tacgttatca ttcactttca tcagagatat accaatggcg cgttccaaaa
     5041 ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg
     5101 gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg
     5161 ctgcacctgc gccgcaagaa agcgcatggg ggccagctgc aactattgcg gcgcgacagt
     5221 ccgctaccgg cactaaaact gatacgccga ttcaaaaagt acctcaatct atttctgtcg
     5281 tcaccgctga agagatggca ctgcatcagc ctaagtcggt gaaagaagcg cttagctata
     5341 cccctggcgt tgctgtggga acccgtggcg catctaatac ttatgattac ctgatcatcc
     5401 gcggatttgc ggcagacggc caaagccaga acaactatct gaatggcctg aagatgcagg
     5461 gcaacttcta taacgatgcg gttattgacc catatatgct ggagcgcgct gaaatcatgc
     5521 gtggcccggt ttccgtgctg tatgggaaaa gcagcccagg cggcctgttg aacatggtca
     5581 gcaagcgccc gaccactgaa ccgttgaaag aagttcagtt taaggccggg actgacagcc
     5641 tattccagac tggttttgac ttcagcgatg cgctggatga tgacggcgtt tactcttatc
     5701 gcctgaccgg ccttgcgcgt tctgcgaatg cccagcagaa gggggctgaa gagcagcgtt
     5761 atgctattgc tccagcgttt acctggcgtc cgaatgataa aaccaatttc actttccttt
     5821 cttacttcca gaacgagccg gaaaccggtt attacggctg gttgccgaaa gaggggactg
     5881 tcgaaccgct gccaaacggt aagcgtctgc cgaccgactt taacgaaggc gcgaagaaca
     5941 acacctattc tcgtaacgag aagatgattg gttatagctt cgaccacgaa tttaacgaca
     6001 cctttactgt gcgtcagaac ctgcgctttg cacaaaataa agtctcgcaa aagagcgtat
     6061 atggctacgg catgtgctca gacccgctct attcttcaaa cccctcttcg agtccatgtg
     6121 ctaacgtgcc gcagtctcaa tggggccata cactgactcg tcagtacgtt atcgataatg
     6181 agaagttaga aaacttctct gttgatactc agttgcaaag caaattcgca accggttcag
     6241 tggatcacac tctgctgact ggcgttgact ttatgcgtat gcgtaatgac atcgattcct
     6301 ggtttggtta tgccggttca gttgcaccat ctgatatcta taatttagac cgtagtgact
     6361 ttgattttgg cgctcacccg aatccgtctg gtccataccg cgttctgctg aaacagaaac
     6421 aaaccggcct gtatgttcag gatcaggcgc agtgggataa agtgctggtt acgcttggcg
     6481 gtcgctatga ctgggccgat cagtcatctt tcaaccgtga ttacggtaat aaatctgaac
     6541 gtgacgacaa agagttcacc tggcgtggtg gtgtaaacta cctgttcgac aacggcgtaa
     6601 cgccttactt cagctacagt gaatcatttg aacccgcttc acagactgat gcgaacggcg
     6661 atctgtttgc accttcgaaa ggcaaacagt atgaagtcgg cgtgaaatat gtgccagaag
     6721 atcgcccaat tgtggtgact ggcgcactgt atcagcttac caaaactaat aacctgatgg
     6781 cggatccgaa cggttcactg ttctcggttg aaggcggtga gattcgtgcg cgtggcgtag
     6841 agctggaagc gaaagcggca ctgtcggcga gtgttaactt ggttggttct tacacctata
     6901 ctgacgcgga atacaccact gacaccaact acaaaggaaa tacgcctgca caggtgccaa
     6961 aacacatggc ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtctga
     7021 cgctgggcac cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta
     7081 aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca
     7141 tggcgggctc caacgtggcg ctgcatgtca acaacctgtt cgatcgtgaa tacgtcgcca
     7201 gctgctttaa cacttatggt tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa
     7261 ccttccgttt ctaatttctc tttttggggc acggatttcc gtgcccgttt cacaagttgg
     7321 ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt aatatctcct
     7381 ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct gccgggaaag
     7441 tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa atgcttggtc
     7501 gtcaccagcc accgtcggaa ggggagattc ttattgatgc ccaaccgctg gaaagctgga
     7561 gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct ccggcagaag
     7621 ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc gcgctggggc
     7681 gctttggtgt tgcagatcgc gaaaaggtcg aggaagctat ctcgctggtt ggcttaaaac
     7741 cgctggcgca tcggctggtc gatagccttt ctggcggcga acgtcagcgg gcgtggatcg
     7801 ccatgctggt ggcgcaggat agccgctgtc tgttgctcga cgaaccgact tcggcgctgg
     7861 atatcgccca ccaggttgat gtactggcgc tggtgcatcg tttaagtcag gagcgtggcc
     7921 tgacggtcat tgccgtgctg cacgatatca atatggcggc gcgctactgt gattatctgg
     7981 tcgccctacg cggcggtgaa atgattgctc agggaacgcc tgcggaaatt atgcgcggcg
     8041 aaaccctcga aatgatttat ggcatcccga tgggtattct gccgcatccg gcgggtgctg
     8101 cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc gccgactgtt
     8161 aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg cggcggttat
     8221 tgatcccaat cgtattgtgg cactggagtg gttgccggtg gagttactgc tggcgctcgg
     8281 catcgtgcct tacggcgtgg cggacaccat caactatcgc ctgtgggtca gcgaaccgcc
     8341 attgccggac tcagtgattg acgttggttt gcgtacagaa cccaaccttg aactgctgac
     8401 cgaaatgaaa ccgtcgttta tggtctggtc tgcaggatat ggcccttcac cagaaatgct
     8461 ggcacgtatt gcgccggggc gcggatttaa cttcagcgac ggcaaacagc cactggcgat
     8521 ggcgcgtaaa tcactgacgg aaatggcaga tttacttaac ctgcaaagcg cagcggaaac
     8581 gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg tgaagcgtgg
     8641 tgcacgcccg ttattgctga cgacgcttat cgatccgcgc catatgttgg tcttcggtcc
     8701 aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct ggcaagggga
     8761 aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcagcgt ataaagacgt
     8821 tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa tggcaacccc
     8881 gctgtggcag gccatgccgt ttgtccgcgc cggacgcttt cagcgcgtac ctgcagtttg
     8941 gttctatggt gcgacgcttt ctgcaatgca ctttgtgcgt attctggata acgccatcgg
     9001 aggtaaagcg tgagtaaacg aattgcgctt tttccggtgt tattgctggc gctgctggtg
     9061 gttgctgctg cggcgttgac ctggatgaac ttctcgcagg cgctgccgcg cagccagtgg
     9121 gcgcaggccg cctggttgcc ggatattgac gtcatcgagc agatgatttt tcactacagc
     9181 ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcgg gcctggggat ggtgggcgtg
     9241 ctgtttcagc aagtgctgcg taacccgctg gcggaaccga cgacgttggg tgttgcaaca
     9301 ggcgcgcaac tggggattac cgtcaccacg ctctgggcga tccccggcgc gatggcgagc
     9361 cagtttgctg cgctggcagg ggcttgtgtt gttggcttaa tcgtctttgg cgtcgcgtgg
     9421 gggaaacggc tttcgccggt aacgctgatc ctcgcggggc tggtagtgag cctttattgc
     9481 ggcgcaatca atcagttact ggttatcttc catcatgacc aactgcaaag catgttcctg
     9541 tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt atggccgcag
     9601 ctgctgggcg gcgtgatgct gacgttattg ctacttcgcc cgttaactct gatggggctt
     9661 gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgctcgtct ggcggcgttg
     9721 tcgctggcga ttgtcatcag tgcgctgctg gtgaacgcgg tggggattat cggctttatc
     9781 ggtttgttcg cgccactgct ggcgaaaatg ctgggggcgc ggcgtttgtt gccacggttg
     9841 atgctggcat cgctgattgg tgcgttaatt ctgtggcttt ctgatcaaat catcctctgg
     9901 ctgactcgcg tgtggatgga agtgtctacc ggttcggtca ctgcgttgat cggtgcgccg
     9961 ctgctactgt ggctgctgcc gcgtttacgc agcattagcg cgccggatat gaaggtcaac
    10021 gatcgtgtcg cgactgaacg ccaacacgtg ctggcgtttg ctctcgcggg cggcgtgctg
    10081 ctgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg
    10141 gcgagcgggg cgttgctcga ggatttgatg ccctggcgct ggccgcgaat tatggcggcg
    10201 ctgtttgcgg gtgtcatgct ggcggtggcg ggctgtatta ttcagcggct gaccggaaac
    10261 ccgatggcaa gcccggaagt gctggggatt agctccggcg cggcgtttgg cgtggtgtta
    10321 atgctgtttc tggtgccggg taatgccttt ggctggctgt tacccacagg cagtctcggg
    10381 gcggcggtga cgctgttgat cattatgatc gccgccggac gcggtggatt ttccccacac
    10441 cgcatgttac tggcggggat ggcgttaagt accgcgttca ccatgctttt gatgatgttg
    10501 caggcaagtg gtgacccgcg aatggcgcaa gttctgacct ggatttccgg ttcgacctac
    10561 aacgcgaccg atgcgcaggt ctggcgtact ggaattgtga tggtgatttt gctggcgatt
    10621 accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac cgcacgagcc
    10681 gtggggatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg
    10741 gcgaccgcga caatgaccat tggtccgttg agttttgttg gtttaatggc accgcatatt
    10801 gcgcggatga tgggctttcg acggacgatg ccacacatcg taatttcggc gctggtggga
    10861 ggattactgc tggtgttcgc tgactggtgt gggcggatgg tattgtttcc attccagatc
    10921 ccagcggggc tgctgtcgac ctttatcggc gcgccatatt ttatctattt gttgagaaag
    10981 cagagccgtt aataggatgc ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa
    11041 acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc gccacagaca
    11101 taaagcccgc ttcaaacgct gacggtgcca gataaacacc ttcgtccagc atcatatgga
    11161 agaaacgttt aaagcgttcc acgtcacagg acatcacatc ctggtagcat gtcacggact
    11221 cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gtttaccacc agcggaattc
    11281 cggcttcttc tgccgcttcc cgcagacctt ctgccagacg tgttgtcagc tcattcagcg
    11341 tctcgtgaac gcccggctgt gcgacttcat tcagacaggc gaaaccagca gccatcgcaa
    11401 tcgggttacc ggaaagcgta cccgcctgat agaccggacc ggtcggggcc agcgcatcca
    11461 ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg attttgccga
    11521 ggcaggtgag atccggttcc acaccgtaat aatcctgtgc gccagctagc gctacgcgga
    11581 aaccggtcat cacttcatcg atgatcagca acgcgccaaa ttcgtcgcac agcgcgcgca
    11641 gacctggcag gaactctggc agcggtggca cgcagttcat attgcctgcc accggctcga
    11701 cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca gaggccagat
    11761 cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc ggcgaatttg
    11821 gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caagcagtca gcgtgaccgt
    11881 ggtaacaacc ttcaaattta ataattttgt cgcgaccggt aaaaccacgg gccaggcgga
    11941 tggcgctcat cgtcgcctcg gtgccggagt tcaccatgcg caccatatcc atggtcggga
    12001 ccagttcggt caccagttgc gccattttca cttccatttc ggttggtgca ccaaagctta
    12061 aaccacgctc ggcggcttca atcacggcat tgcggatcgc cggatggtta tggcccagca
    12121 ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca tcgtacagat
    12181 aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa
    12241 caggggagtt cacgccgcca gggatcagct cgcgcgctgc gctgtaaaga ttttcagact
    12301 tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct atgttattca
    12361 taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt
    12421 agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt tcatggaacg
    12481 tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg gatgaaaact
    12541 gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca actgattcgc
    12601 caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc agtcgtcggc
    12661 acgcttgtcg ggctggctgc ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa
    12721 cgtatggggg cactggtaca tactgctgat aattatccgc ttctgttaac cgtcgctttt
    12781 ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata cgcgccggaa
    12841 gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg aagatcaacg tcctgttcgc
    12901 tggtggcgtg tgttgccggt gaaattcttt ggtgggctgg ggacactcgg cggaggcatg
    12961 gtgttggggc gcgaagggcc aaccgtgcag ataggcggca atatcgggcg gatggtactc
    13021 gatgttttcc gcctgaaagg tgacgaagct cgtcatacgc tactggcaac cggtgctgct
    13081 gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat tatcgaagag
    13141 atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat tggcgtcatt
    13201 atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga cgtcggtaaa
    13261 ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat tatttttggc
    13321 attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct gcaccgtgtg
    13381 cacggtggca atattaccaa atgggtactg atgggcggtg cgattggcgg tttgtgtgga
    13441 ttgctggggt ttgtggcacc agcaacttcg ggcggcggtt ttaacctgat tcctatcgct
    13501 accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgcgcg ggtcattacc
    13561 accttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat gctggcgctg
    13621 ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agttgtttcc gcaatatcac
    13681 cttgaggcgg ggacgtttgc tattgccgga atgggggcgt tactggcggc atctattcgc
    13741 gcgccgttaa cggggatcat tctggttctg gaaatgaccg ataactacca gctcattttg
    13801 ccaatgatta ttaccggtct tggcgcaaca ctattagcac aatttaccgg tgggaaaccg
    13861 ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca actggcgcga
    13921 agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat tagataatgg
    13981 cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca aaatatgagt
    14041 gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt taaaagcctg
    14101 atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc
    14161 agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga tatgaccatc
    14221 gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt
    14281 tccgttgact ataccgaagg tctggaaggt tctcgtttca tcgtgaccaa cccgaacgcg
    14341 aaaagcacct gcggttgcgg ttcttccttt agtatctaat ctgctgttca tgtgccggat
    14401 aaactatccg gcacatctcc tctcttcaac gaccattctc atccagcgca aacgtcggta
    14461 gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc atgcccatca
    14521 tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg acaataccgc
    14581 cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt tcgcgggcca
    14641 gtacatcacg aatgattccg ccgccaacgc cagtaatgac gcccatacaa accgcgatta
    14701 acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac accgccagac
    14761 caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg cgcaccagca
    14821 cgatggtcag catgctggtg accattgcaa cgaccagatc ggtgggatct ttcacccaaa
    14881 ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gccgcctact gcggtaacca
    14941 cgcccaatac cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccag
    15001 agatagcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc
    15061 cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt acagagctgt
    15121 tgtgcagcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata
    15181 acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg
    15241 cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct aacttgcggc
    15301 cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttc
    15361 tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt
    15421 tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag cagggattgc
    15481 gcggcttgtt cggccttatc cggttgcgga ctccaggggg ccagttgacg taacgcattg
    15541 gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc
    15601 tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc
    15661 gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct
    15721 tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca
    15781 agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcattgag ccacagcggc
    15841 gcgagaaaag acagggcgac cagcgccctg aacagtgact tagccatgtg ccagtttctg
    15901 caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga actcatcgaa
    15961 gctaagatga gactgttgat cggccacgtc ggagatggcg cgtactacga caaacgggac
    16021 gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg
    16081 tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct
    16141 aacaatcagg ccacgtacag cgttaagatt cagttcggca atgcaggcct cagcggcagc
    16201 gatcagttta tcgtcagctt tgaagcctgc cgggcagcct ggtaactgac cgtattcata
    16261 accaaatgcc gtgacatccg cgtcgtgata acgcgcttcg tccgagacaa cgatatcgcc
    16321 cactttcagc gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt
    16381 gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga tgcccgattt
    16441 cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac cgcccagact
    16501 gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc
    16561 aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc atagtttacc
    16621 atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc
    16681 gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg
    16741 agattctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc
    16801 gtctgcaaca aaagacccag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc
    16861 ttacccactc gatggaagtc cagcaggtgg ggcgttatat cgctaaagag attttaagcc
    16921 gcctgaaaga gcttaaatta ctggagacgt atggtctcga tgaactgacc gggccctttg
    16981 aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg tttggtcatt
    17041 ttggcgaagc ggcgataaat gactggtttc gccagcgttt gtacccggaa gatgccgaaa
    17101 gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg gacggggaag
    17161 aaccgcttaa cgcgctgcgg cgcaagattc gtcaggactt atgtcatttt gaaggtaacg
    17221 cacagggtat tcgtttggtg catacattga tgcggatgaa tctcacctgg gcgcaggttg
    17281 gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg tgaaacgcct gagacacatc
    17341 actatttaat gaaaaagccg ggctattatc tttccgaaga agcctatatt gcccggttgc
    17401 gtaaagaact taatttggcg ctttacagtc gttttccgtt aacgtggatt atggaagctg
    17461 ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa aggatattta
    17521 ccgttgagca gctttatcat catttgcacg aggcgtgggg ccagcatgag aaaggttcgc
    17581 tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca
    17641 gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa ctggtaccct
    17701 acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg tttaatcatg
    17761 cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa aatgtcgctg
    17821 taaaacatgt gtttagccat ccagatgtcg agcagcttga attgcagggc tatcgggtca
    17881 ttagcggatt attagagatt tatcgtcctt tattaaacct gccgttatca gactttactg
    17941 aactggtaga aaaagaacgg gtgaaacgtt tccctattga aacgcgctta ttccacaaac
    18001 tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg tcagattctc
    18061 ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat tatatcagcg
    18121 gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta gaacaataac
    18181 caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt agttcggaac
    18241 ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt taatcgagac
    18301 tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt ttaggtttgg
    18361 cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca gcccagcaga
    18421 tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc attaacgtag
    18481 aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg
    18541 atgattctcc gttctgccag gagggttctc cgttccagag ctctccgttc tgccaggggg
    18601 gcccgggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt tccggcgtca
    18661 tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg
    18721 tgattaaagt ccagcttagc gatggtcgta agttcgacgc gaagatggtt ggcaaagatc
    18781 cgcgctctga tatcgcgttg atccagatcc agaacccgaa aaacctgacc gcaattaaga
    18841 tggcggattc tgatgcgctg cgcgtgggtg attacaccgt agcaattggt aacccgttcg
    18901 gcctgggcga gacagtaact tccgggattg tctctgcgct gggacgtagc ggcctgaacg
    18961 ctgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccggggt aactcaggtg
    19021 gtgcgttggt taacctgaac ggcgaactga ttggtatcaa caccgcgatc ctcgcaccgg
    19081 acggcggaaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa aacctgacct
    19141 cgcagatggt ggaatacggc caggtgaaac gtggtgagtt gggtattatg ggcactgagc
    19201 tgaactccga tctggcgaaa gcgatgaaag ttgacgctca gcgcggtgct ttcgtaagcc
    19261 aggttctgcc gaattcttcc gccgcgaaag cgggcattaa agcgggtgat gtgatcacct
    19321 cactgaacgg taagccaatc agcagctttg ccgcactgcg tgctcaggtg ggcactatgc
    19381 cggtaggtag caaactgacc ctgggcttac tgcgcgacgg gaagcaggtt aacgtgaacc
    19441 tggaacttca gcagagcagc cagaatcagg ttgattccag caccatcttc aacggcattg
    19501 aaggcgctga gatgagtaac aaaggcaaag atcagggcgt ggtggtgaac aacgtgaaaa
    19561 cggggactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt ggcgcgaacc
    19621 agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc
    19681 tggcactgaa cattcagcgc ggcgacagca ctatctacct gttaatgcag taatctccct
    19741 caaccccttc ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt caccacaact
    19801 ccatacatct tcatcatcct ttgggcattt gcacaatgcc gtacgttacg tacttcctta
    19861 tgctaagccg tgcataatgg aggacttatg gctggttggc atcttgatac caaaatggcg
    19921 caggatattg tggcacgtac catgcgcatc atcgatacca atatcaacgt aatggatgcc
    19981 cgcgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca cgaaggagca
    20041 ttgctggtac tttcacaagg acgagtcgtc gatatcgatg acgcggtggc acgtcatctg
    20101 cacggtgtgc ggcaggggat taatctaccg ttacggctgg aaggtgaaat tgtcggcgta
    20161 attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt ctgcatgacg
    20221 gctgaaatga tgctggaaca gtcgcggttg atgcacctgt tggcgcagga tagccgtttg
    20281 cgggaagaac tggtgatgaa cctgattcag gctgaggaga atactcccgc acttactgaa
    20341 tgggcgcaac ggctggggat cgatctcaat caaccgcgag tggtggctat tgttgaggtc
    20401 gacagcggtc agcttggtgt ggacagcgca atggcggagt tacagcaact gcaaaatgcg
    20461 ctgactacgc ccgagcgtaa taatctagtg gcgattgtct cgctaaccga aatggtggtg
    20521 ttgaaaccgg cgttgaactc ttttgggcgc tgggacgcag aagatcatcg taagcgagtt
    20581 gaacaactga ttacccgcat gaaagagtat ggccagctgc gttttcgcgt ttcgctgggc
    20641 aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa aacgacgatg
    20701 gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga tctgatgtta
    20761 cctgtgttac tcgacagttt gcgtggcgac tggcaggcca acgaactggc gcgaccgctg
    20821 gcgcggctga aagcgatgga caataacggc ttgctgcggc gaactctggc ggcgtggttt
    20881 cgccacaatg tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg taatactctg
    20941 gaatatcggc ttaatcgtat atcggaactg accgggcttg atttgggcaa ttttgatgac
    21001 aggttgctgc tgtatgtggc gttacaactg gatgaagagc ggtaggtcga ttacaaaatt
    21061 acagccgggt gagactttac ccggcccaca gaatgacatt atttacgcgt cagtttttcc
    21121 agatcggctt caatctcgct gatcttattg gttactactg actccaggtg acgtaagtca
    21181 tcgaggatct tacgcttaag atcaacttcg ctgcggtcac gctggcagat ttgatcaagc
    21241 tcatcaatga tataccgtag attcgggctg atttcctgga cttctttata accctgcccc
    21301 acaccatcag cgacaaccgt tttacgctga cgcggatatt taaacttcac gctcttggcg
    21361 aaaaactcgc ctttgtcttt ctggaaatag attttcagaa tatcgttgtt ggcttcctgc
    21421 cggaggctgt aacgatcaat ttcttcagga ttggtaatac ccagactttt cagattgtcg
    21481 tacatagcgt tacctcaaaa tgagtcagta aattgtgctt attttagcat ttggcctggc
    21541 ccgcccggct cgactgtgat cgcagaaagc ctttttcttc cagacttcta tcatggcgta
    21601 aaaatcaaaa aattacctgc tttattgtga tgatattcca tgggatggat tagcacattt
    21661 atcttcattt tgcgaattac atgacagtcc gaaacaagat gaaaacgtat ttaaaaaagg
    21721 gggaaaatga gtaggacgaa ttattctgag ccagaaagca atcgggcagc gtcgtgctgc
    21781 ccgtgtacat acttttagtc gatggtacgc agcagttcgt taataccaac tttaccgcga
    21841 gttttcgcgt caactttctt aacgataact gcacagtaga gactgtattt gccatctttc
    21901 gacggcagat tacctgaaac aaccacagac cctgccggaa cgcgaccgta gtggatttcg
    21961 ccggtttcac ggtcataaat acgggtgctc tgaccaatgt atacgcccat agagatgacg
    22021 gaaccttctt cgacaatcac cccttcaacc acttcagagc gtgcgccgat gaagcagtta
    22081 tcttcaataa tggtcgggtt agcctgcagc ggctccagca cgccgccgat acccacgcca
    22141 ccggaaaggt ggacgttttt accaatctgc gcacaggaac cgacggtcgc ccaggtatca
    22201 accatggtgc cttcatcaac atatgcgccg atgttgacgt aagacggcat cagcacggtg
    22261 ttacgggcaa tgaacgcgcc ctgacgcact gccgctggtg gcacgacgcg gaagccttct
    22321 ttctggaaac gtgcttcatc gtagttagcg aatttcatcg gcactttgtc gaagtagcgg
    22381 ctttctgccc cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt
    22441 ttcaaccact gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcgccg
    22501 gaatccagca gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcattggct
    22561 ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt
    22621 gttaaactct tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct
    22681 gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg
    22741 gcaattatga ataaatcttc tactcgctcg ccaatggttg taattcgggc gccatgaagc
    22801 gaaattccca gatcggcaaa aattttcccg actcgcgcca gcagtccagg ttggtcgagg
    22861 gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt cacttcggtt
    22921 tcaacagtaa aatggcgcaa tttagcgggt tggcgacggg gctgcggtgg ctgccagcta
    22981 ctttgtgtca gcacctgctc cagaccaaac cgaataacct catgacgatc tgcggatagc
    23041 gggctaccat cgggttccag cactataaag gtatccatcg ccataccgtc gcgagtggtg
    23101 aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca
    23161 aacagatagg ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc
    23221 gggctaagca gtaccagcgg tttgcttaaa tcatgctgta ataaatggcg tgcgtgccag
    23281 gccagttgat ttgggctatg gcggacaaaa tagttagcgc gacagcgtga ccaaatttgg
    23341 tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg ccagcgcctg gagttggtga
    23401 tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg
    23461 gtggcaaaat agagttcgcg caacagactt tgcttccagc tattccacag tgtttcgttg
    23521 gtggcgcaaa tgtcagccac tgtcaggcat accagatagc gcagacgatt ttccgtttgg
    23581 acttcttcgg caaactgctt gatgacttcc gggtcctgaa tatcgcggcg ctgagctgtc
    23641 accgacatca gcaagtgctg gcgaaccagc caggcgacca gctgtgtttc gcgtgagttc
    23701 agcccgtgga gttcggcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg
    23761 ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc
    23821 agacgcggcc agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa
    23881 ctctccagtt tcaacatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga
    23941 tcaaactgca tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagtacgcta
    24001 tggcgatgca ttggcaatag cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa
    24061 aacagttttc gcgcttccgg aatattacac agaggttgtt gcagatggcg acgggcgtgg
    24121 cgtaactggc gtagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg
    24181 tagaacatac gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg
    24241 atcagcgttc cgcgtagctg aaactcatcg tcgattggac gtggtttttc gtcggcggga
    24301 agggcgagga tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg
    24361 cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg gttcgttacc ttcaccactg
    24421 taattcaaac gctgggcgac gctaagctgg cgatcgaata acaggcgatt atcgtaacga
    24481 ctgacgacca gatgcagggc aaagcgaatg cgccacaata tatgcaggca ttcgtttaat
    24541 tccgctcgct ccgcagaggt taagaagcca aaccctacca tttcatccag cgatgttgcg
    24601 ccaaaatgac ggcgggccac ccattgcaga gtgtggatat cgcgcaagcc gccggggcta
    24661 cttttgatgt ctggttcaag gttgtagctg gtgccgtggt aacgctgatg gcgctggttc
    24721 tgttcttcaa ctttcgccgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata
    24781 tgtttttgca gttcgaggaa cagcgcaaca tcgccaatta ataagcggga ttcgattaaa
    24841 ttggtggcga cggttaaatc cgataaccct tccagcatgc actcttcaag cgtgcgcacg
    24901 ctatgaccca cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc
    24961 gcctgatcgt ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga
    25021 tgcagctcgc cacgaccgta gccaccgacg gcgaccaatg ccaggtcggc aatctggctg
    25081 aatcccgctt caatccataa tcgttgaaga agctggtcga taaactcggt acgcgcctca
    25141 atcaactgtt ctgcggagat accattgtca aaggcatcac ccagccaacg ctggaaagta
    25201 tcgatatggg cttttatccc accgacggtt aattcatcac ggggccaggc gcatggattt
    25261 tgcggttgac cgtgcagggt ggggagagcg gtgtttgcgt actgttctgg aagggtattc
    25321 attgtgcgcc acccataaga ttaaattatt gcattaaaaa agccggcatt cgccggcttc
    25381 attttattcg tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat
    25441 ttcgcagccg ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc
    25501 tttggttttt accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac
    25561 cattggctcg atggtgaatg tcatccccgg tttcagcacg acgttggttt cacgggagtc
    25621 atagtgcagc acctgcggtt cttcgtggaa gccgcgacca ataccgtgtc cgcaatattc
    25681 acgaacgacg gagaagcctt ctgcttcgac aaatttctga attgccgcac cgatttcgcg
    25741 cagattaatg cctggtttta ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg
    25801 gcacagacgt tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc
    25861 gtggaaacca tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag
    25921 cttggcgtcg tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt
    25981 cggatagccg tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc
    26041 attacagatg cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat
    26101 ctccagcact tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt
    26161 cttgattgag atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg
    26221 taagtgccgt caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa
    26281 cgtgccactg aaggttttct ataatagaaa attcaacgtc tgatgctgta cacagcgcca
    26341 acaattattg gtgtccacga cgtatttgtg gtataaagcg cgccggactt ccgatccatt
    26401 tcgtatacac agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc
    26461 ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc
    26521 caacttttat atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc
    26581 tggtgttcac ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt
    26641 cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga
    26701 agctctggct gaactgaaca agattgcttc tcgcaaaggt aaaatccttt tcgttggtac
    26761 taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt
    26821 gaaccatcgc tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa
    26881 acgtctgaaa gacctggaaa ctcagtctca ggacggtact ttcgacaagc tgaccaagaa
    26941 agaagcgctg atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa
    27001 agacatgggc ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc
    27061 tatcaaagaa gcaaacaacc tgggtattcc agtatttgct atcgttgata ccaactctga
    27121 tccggacggt gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct
    27181 gtacctgggc gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca
    27241 ggcggaagaa agcttcgtag aagctgagta ataaggcacg ctcgtacgag cccttataga
    27301 ccaggtagta cacgtttggt tagggggcct gcatatggcc ccctttttca cttttatatc
    27361 tgtgcggttt aatgccgggc agatcacatc tccgaggatt ttagaatggc tgaaattacc
    27421 gcatccctgg taaaagagct gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa
    27481 gcactgactg aagctaacgg cgacatcgag ctggcaatcg aaaacatgcg taagtccggt
    27541 gctattaaag cagcgaaaaa agcaggcaac gttgctgctg acggcgtgat caaaaccaaa
    27601 atcgacggca actacggcat cattctggaa gttaactgcc agactgactt cgttgcaaaa
    27661 gacgctggtt tccaggcgtt cgcagacaaa gttctggacg cagctgttgc tggcaaaatc
    27721 actgacgttg aagttctgaa agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa
    27781 attggtgaaa acatcaacat tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct
    27841 tatcagcacg gtgcgcgtat cggtgttctg gttgctgcta aaggcgctga cgaagagctg
    27901 gttaaacaca tcgctatgca cgttgctgca agcaagccag aattcatcaa accagaagac
    27961 gtatccgctg aagtggtaga aaaagaatac caggtacagc tggatatcgc gatgcagtct
    28021 ggtaagccga aagaaattgc agagaaaatg gttgaaggcc gcatgaagaa attcaccggc
    28081 gaagtttctc tgaccggtca gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg
    28141 ctgaaagagc ataacgctga agtgactggc tttatccgct tcgaagtggg tgaaggcatc
    28201 gagaaagttg agactgactt tgcagcagaa gttgctgcga tgtccaagca gtcttaatta
    28261 tcgaaaagga gccgcctgag ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac
    28321 ccttgtgggg ctgcgctgaa aagcgacgta caatgtcgct agtattaatt catttcaatc
    28381 gttgacagtc tcaggaaaga aacatggcta ccaatgcaaa acccgtctat aaacgcattc
    28441 tgcttaagtt gagtggcgaa gctctgcagg gcactgaagg cttcggtatt gatgcaagca
    28501 tactggatcg catggctcag gaaatcaaag aactggttga actgggtatt caggttggtg
    28561 tggtgattgg tgggggtaac ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc
    28621 gcgttgtggg cgaccacatg gggatgctgg cgaccgtaat gaacggcctg gcaatgcgtg
    28681 atgcactgca ccgcgcctat gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg
    28741 tgtgcgacag ctacagctgg gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga
    28801 tcctctccgc cggtacaggt aacccgttct ttaccaccga ctcagcagct tgcctgcgtg
    28861 gtatcgaaat tgaagccgat gtggtgctga aagcaaccaa agttgacggc gtgtttactg
    28921 ctgatccggc gaaagatcca accgcaacca tgtacgagca actgacttac agcgaagtgc
    28981 tggaaaaaga gctgaaagtc atggacctgg cggccttcac gcttgctcgt gaccataaat
    29041 taccgattcg tgttttcaac atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg
    29101 aaaaagaagg gactttaatc acggaataat tcccgtgatg gataaataag ggtaagattc
    29161 cgcgtaagta tcgcggggga tcttacccgt aatatgttta atcagggcta tacttagcac
    29221 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    29281 gattagcgat atcagaaaag atgctgaagt acgcatggat aaatgcgtag aagcgttcaa
    29341 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    29401 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    29461 ttcccgtact ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    29521 gattatggcg tccgatcttg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    29581 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    29641 agaacaagcg cgtgttgcag tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc
    29701 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    29761 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    29821 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    29881 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    29941 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    30001 ccgaacattt ccgcgtagtt gcgctggtgg ccggcaaaaa tgtcactcgc atggtagaac
    30061 agtgcctgga attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc
    30121 ttaaaacgat gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg
    30181 cttgcgatat ggcagcgctt aaggatgttg atcaggtgat ggcagccatt gttggcgctg
    30241 ctggactgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    30301 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    30361 aattgttacc ggtcgatagc gaacataacg ccatttttca gagtttaccg caacctatcc
    30421 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    30481 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc
    30541 aagcctgccg tcatccgaac tggtcgatgg gacgtaaaat ttccgtcgat tcggctacca
    30601 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    30661 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc taccaggacg
    30721 gcagtgttct ggcgcagctg ggtgaaccgg atatgcgtac gccaattgct cacaccatgg
    30781 catggccgaa tcgcgtgaac tctggcgtga agccgcttga tttttgcaaa ctaagtgcgt
    30841 tgacatttgc cgcaccggat tatgatcgtt atccatgtct gaaactggcg atggaggcgt
    30901 ttgaacaagg ccaggcagcg acgacagcat tgaatgccgc aaatgaaatc accgtggctg
    30961 cttttcttgc gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg
    31021 aaaaaatgga tatgcgcgaa ccacaatgtg tggatgatgt gttatctgtt gatgcgaacg
    31081 cgcgtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    31141 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    31201 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    31261 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aagaaacgcg tgatgttgtc
    31321 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgtcgtcatg ttgcgatcat
    31381 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    31441 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    31501 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    31561 gttaatggaa ctgtttgtgt gggcgctaga tagcgaagta aaaagtctgc accgacataa
    31621 cgtgcgtctg cgtattattg gcgataccag tcgctttaac tcgcgtttgc aagaacgtat
    31681 tcgtaaatct gaagcgctaa cagccgggaa taccggtctg acgctgaata ttgcggcgaa
    31741 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    31801 aggaaacctg caaccagatc agatagatga agagatgcta aaccagcatg tctgtatgca
    31861 tgaactggcc cctgtagatt tagtaattag gactgggggg gagcatcgca ttagtaactt
    31921 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    31981 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    32041 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    32101 gatatctgct tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt
    32161 ggggttcgcc attgtaacgc tggtggtctg tatgctggca gcgtgggaat ggggacagct
    32221 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    32281 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    32341 aatctcactt tgggcttcgc tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    32401 cccaggttcc gcagcaatct ggcgtaactc taaaacattg cgcattattt ttggcgtgct
    32461 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact acgacgagaa
    32521 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    32581 tggcgcatat atgtttggca aattgtttgg taaacataaa ctggcaccga aggtttctcc
    32641 gggtaaaacc tggcaaggct ttatcggtgg actcgtgacc gcagcggtaa tctcatgggg
    32701 ttatggcatg tgggtgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    32761 cgcagcgtta gcctcagtgc tcggcgatct gaccgaaagt atgtttaagc gtgaagcggg
    32821 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    32881 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    32941 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    33001 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    33061 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    33121 accgaatatg ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc
    33181 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    33241 cgagcggcga ttattgccgc aggtccagtt gcaaacttca tttttgctat ctttgcctac
    33301 tggctggttt ttattattgg tgtgcctggc gtacgtccgg tggttggtga aatagcagcc
    33361 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    33421 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    33481 agcaccacca ttacggtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat
    33541 ttacgtcact gggcgtttga gcctgataaa gaagatccgg tatcttcgtt ggggattcgt
    33601 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc
    33661 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    33721 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    33781 gaaagacagg ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    33841 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgcctga tgagtataaa
    33901 gttgtacgcc agtatgggcc gttcaacgct atcgtcgaag ccacggacaa aacgtggcag
    33961 ttgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    34021 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta
    34081 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    34141 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    34201 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg
    34261 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    34321 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    34381 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    34441 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg cacaggcgac acggttaatg
    34501 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    34561 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    34621 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    34681 cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    34741 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga
    34801 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    34861 aaatccagca aattaacatt gttggtaacc atgctttcac caccgatgaa ctgatctctc
    34921 atttccaact gcgtgacgaa gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga
    34981 aacagaaact ggcgggcgac cttgaaaccc tgcgcagtta ctatctggat cgcggttatg
    35041 cccgtttcaa catcgattct acccaggtca gtctgacgcc agataaaaaa ggtatttacg
    35101 tcacggtgaa catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    35161 atcttgccgg gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt
    35221 ataacggcac caaagtgacc aaaatggaag atgacattaa aaagcttctt ggtcgctatg
    35281 gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta
    35341 aattacgcgt gaacgttgat gcgggtaacc gtttctatgt gcgtaagatc cgttttgaag
    35401 gtaacgatac ctcgaaagat gctgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    35461 ggctggggag cgatctggtc gatcagggta aggagcgtct gaaccgtctg ggcttctttg
    35521 aaactgtcga taccgatacc caacgtgttc cggggagccc ggatcaggtt gatgtagttt
    35581 acaaggtgaa agaacgtaac accggtagct tcaacttcgg gattggttac ggtactgaaa
    35641 gtggcgtgag cttccaggct ggtgtgcaac aggataactg gttaggtacg ggttatgctg
    35701 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    35761 cgtacttcac cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg
    35821 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    35881 gcttcccgat caacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    35941 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    36001 cgagcacctc tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt
    36061 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    36121 ccggtaaagt gaccattccg ggatcggata acgaatacta caaagtgacg ttagacacgg
    36181 cgacttatgt gccgattgat gacgatcaca aatgggttgt tctgggtcgt acccgttggg
    36241 gttatggtga tggtttaggc ggcaaagaga tgccgttcta tgagaacttc tatgccggtg
    36301 gttccagcac cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc
    36361 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    36421 cgaaagatct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    36481 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    36541 ccttcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    36601 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    36661 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    36721 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    36781 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    36841 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    36901 ttattagctg caggtctcgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    36961 gcaatcgtca atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac
    37021 acgctggaaa acgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    37081 caggctaaaa tgaaaaagct gcagtccatg aaagcgggca gcgatcgcac taagctggaa
    37141 aaagacgtga tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    37201 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    37261 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    37321 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    37381 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    37441 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc
    37501 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    37561 acccaggacg atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg
    37621 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    37681 cccagtgcgg tgattgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    37741 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    37801 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    37861 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    37921 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgggta
    37981 attattggcg atcgcgtgga gatcggtgcc tgcactacta ttgatcgtgg cgcgctggat
    38041 gacactgtta ttggcaatgg tgtgatcatt gataaccagt gccagattgc acataacgtc
    38101 gtgattggcg acaatacggc agttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    38161 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    38221 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    38281 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    38341 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    38401 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    38461 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    38521 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    38581 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    38641 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    38701 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    38761 tggaaccggg tgagctgtac tacttcgccg gtattgacga agcgcgcttt aagcgcccgg
    38821 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    38881 cccgttttaa aggggttgct ctggtcgacg gtaaagtagt ttgcgaagca acgatgatgt
    38941 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    39001 ccattgtgga agagggcgcg tcgattggcg cgaacgctca cattggtcct ttttgtatcg
    39061 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    39121 gtcatactaa aattggccgc gataatgaga tttatcagtt cgcctccatc ggcgaagtta
    39181 accaggatct gaaatatgct ggcgaaccga cccgcgtgga aatcggcgat cgtaaccgca
    39241 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    39301 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    39361 gctgcatcct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    39421 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg
    39481 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg
    39541 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    39601 ttaccgctat ccgcaatgcg tataagctga tttatcgtag cggtaagacg ctcgatgaag
    39661 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    39721 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaacagcgtc cattaacgat
    39781 tgccctggtc gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct
    39841 gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    39901 aggctgcgaa gcctggtacg aaatggaaga actggcagtg atgggcattg ttgaagtgct
    39961 cggtcgtctg cgtcgcttac tacatattcg tgccgatctg acaaagcgtt ttggcgaact
    40021 gaagccagat gtttttgttg gtattgatgc gcctgacttc aatattactc ttgaaggtaa
    40081 cctcaaaaag cagggtatca aaaccattca ttacgtcagt ccgtccgtct gggcgtggcg
    40141 acagaaacgc gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    40201 cgaaaaagcg ttttatgaca aatacaacgt accgtgccgc tttatcggtc ataccatggc
    40261 tgatgccatg ccattagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta
    40321 cgatgcccac tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag
    40381 tgccgatttc ctgaaaacgg cccagctttt gcgccagaca tatccggatc tcgaaatcgt
    40441 ggtgccgctg gtgaatgcca aacgccgtga gcagtttgaa cgcatcaaag ctgcagtcgc
    40501 gccagacctg tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagtga
    40561 tgcggcgcta ctggcgtcgg gtacggcagc actggagtgt atgctggcta aatgcccgat
    40621 ggtagtggga tatcgcatga agcctttcac cttctggctg gcgaagcgac tggtgaaaac
    40681 tgattatgtc tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca
    40741 ggaagagtgt gagccgcaaa aattggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    40801 aaccagccat gcgatgcacg acaccttccg cgagctgcat cagcagatcc gctgcaatgc
    40861 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtctatccg
    40921 cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    40981 gtcaccgctg cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    41041 aagctgagcg aaaaacgccg tctggcgctc tgtgaagaga tcaaagagaa agcgttgagc
    41101 tggagtctgg gccgcgcgga acctcacgaa atcgacgaac tgaatatttt acatgcgacc
    41161 atgctggcga tgcagcgtgc cgtcgctgga ctgcatattg cgccggaata tgtgttgatt
    41221 gatggtaacc gctgcccgaa attaccgatg ccttcgatgg ctgtggtgaa aggcgatagc
    41281 cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    41341 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacacaaagg gtatccaacc
    41401 gcttttcatc tggaaaaact ggctgaacac ggcgcgaccg aacaccatcg gcgcagcttt
    41461 gggcctgtca aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    41521 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag tgactactcg
    41581 atgatcgatg gcctggccaa aaccgcaccg ctggtaaaaa aggcggcagc gttgggtatg
    41641 ccagcactgg cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg
    41701 ggacatggcg cagggattaa gcccatcgtc ggggcggatt ttaacgtcca gtgcgacctg
    41761 ctgggtgatg agttaactca cctgacggtg ctggcggcga acaataccgg ctatcagaat
    41821 ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc
    41881 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg
    41941 ggcgacgttg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg
    42001 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    42061 gacgaagaaa gctatctgca cgctgcggtg gaactggcgg aatcacgcgg tttgcccgtt
    42121 gtggcgacca acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    42181 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    42241 cagcaatata tgcgtagcga agaggagatg tgcgagcttt ttgccgacat tcccgaagcc
    42301 cttgccaaca ccgttgagat agccaaacgt tgtaacgtaa ctgtgcgtct gggtgaatac
    42361 ttcttgccgc aattcccgac cggggacatg agtaccgaag attatctggt caagcgtgca
    42421 aaagaaggcc tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaag
    42481 cgtcgtccgg agtatgacga acgtctggat actgaacttc aggttatcaa ccagatgggc
    42541 ttcccgggtt acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggtgta
    42601 ccggtagggc caggccgtgg ttctggtgca gggtcactgg tggcctacgc gttaaaaatc
    42661 accgatctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    42721 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgtgacca ggttatcgaa
    42781 catgtggcgg acatgtacgg tcgcgatgcg gtatcgcaga ttatcacctt cggtacgatg
    42841 gcggcgaaag cggtgatccg cgacgtaggc cgtgtattgg ggcatccgta cggatttgtc
    42901 gatcgtatct cgaaactgat cccgcccgat ccgggtatga cgctggcgaa agcgtttgaa
    42961 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    43021 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    43081 atcgcgccga ccaaaattac tgattttgcg ccgctttatt gcgatgaaga gggcaaacat
    43141 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg ggctggtgaa gttcgacttc
    43201 ctcggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    43261 gcgaagaatg gcgagccccc gctggatatc gccgcgatcc cgctggatga caagaaaagc
    43321 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg
    43381 aaagacctga tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca
    43441 ctgttccgcc ccggcccgtt gcaatcaggg atggtggata actttatcga ccgtaaacat
    43501 ggtcgcgaag agatctccta tccggacgtg cagtggcagc atgaaagcct gaaaccggta
    43561 ctggagccaa cctacggcat catcctgtat caggaacagg tcatgcagat tgcccaggtg
    43621 ctttctggtt ataccctcgg tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag
    43681 ccggaagaga tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    43741 aacgccgaac tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt
    43801 aacaaatcgc actcggcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaggca
    43861 cattatccgg cagagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag
    43921 gtggtgggcc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    43981 aactccggtc tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc
    44041 gcgatcaaag gggtaggtga aggcccgatt gaggccatca tcgaagcccg taataaaggc
    44101 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg
    44161 cgggtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgtgcg
    44221 gcgctgatga actcgctggg cgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    44281 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa
    44341 tcctacgcca gctgccaacc gtggccggag caggtggtgt tagatgggga acgtgaaacg
    44401 ttaggtctgt acctcacggg acaccctatc aaccagtatt taaaagagat tgagcgttat
    44461 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    44521 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    44581 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    44641 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    44701 gatgacttca gcggtgggct taaaatgact gctcgcgaag tgatggatat tgacgaagcc
    44761 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    44821 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    44881 ctctactatc agagggcgga tgcacgcgcg cgattgcgtt ttggcgcgac gtggcgtgtc
    44941 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    45001 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    45061 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    45121 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg
    45181 cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccacag
    45241 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    45301 gaccgcgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt
    45361 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    45421 tttggtatgc cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa
    45481 cgttttaaga tgcctatcat cacctttatc gacaccccgg gggcatatcc gggcgtgggc
    45541 gcagaagaac gtggtcagtc ggaagccatt gcacgcaacc tgcgtgaaat gtctcgcctc
    45601 ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt ctggtggtgc gctggcgatt
    45661 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    45721 ggttgtgcgt ccattctgtg gaagagcgct gacaaagcgc cgctggcggc tgaagcgatg
    45781 ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg
    45841 ctgggcggtg ctcaccgtaa cccggaagcg atggcggcgt ctctgaaagc gcagctgctg
    45901 gcggatctgg ccgatctcga cgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    45961 cgcctgatga gctacggcta cgcgtaatcg gcaaatgttc tgaaaagggt cacttcggtg
    46021 gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga
    46081 acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat
    46141 caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag
    46201 cgttgatttg ctgaagttta tcgaacataa cccacgaatt tgcggcgtga tttttgactg
    46261 ggatgagtac agtctcgatt tatgtagcga tatcaatcaa cttaatgaat atctcccgct
    46321 ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc
    46381 gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca
    46441 gtacaccaac gaatatcttg ataacattac gccgccgttt acgaaagcct tgtttaccta
    46501 cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggta ccgcatatca
    46561 aaaaagcccg gttggctgtc tgttttatga ttttttcggc gggaataccc tcaaggcgga
    46621 cgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc cacacctgga
    46681 agcggaagag tacatcgcgc ggacgtttgg cgcggaacag agttatatcg ttactaatgg
    46741 aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt
    46801 gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg atgtagtgcc
    46861 agtctggctg aaaccgacgc gtaatgcgtt ggggattctg ggtgggatcc cgcgcggtga
    46921 atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac aatggccggt
    46981 tcatgcggtg atcaccaact ccacctatga tggcttgctc tacaacaccg actggatcaa
    47041 acagacgctg gatgtcccgt cgattcactt cgattctgcc tgggtgccgt acacccattt
    47101 tcatccgatc taccagggta aaagtggtat gagcggtgag cgtgttgcgg gaaaagtgat
    47161 cttcgaaacg cagtcgaccc acaaaatgct ggcggcgttg tcccaggcgt cgctgatcca
    47221 cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac
    47281 ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa
    47341 tccgggcaaa cggctgatta accgttcagt agaacgagcg ctgcattttc gcaaagaggt
    47401 ccagcggctg cgggaagagt ctgacagctg gtttttcgat atctggcaac cgccgcaggt
    47461 ggatgaagcc gaatgctggc acgttgcgcc tggcgaacag tggcacggct ttagcgatgc
    47521 ggatgccaat cacatgtttc tcgatccggt taaagtcact attttgacac cgggaatgga
    47581 cgagcagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa aattcctcga
    47641 cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat
    47701 tggcatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc
    47761 ttacgatctc aacctgcgga tcaaaaatat gctgcccgat ctctatgcag aagatcccga
    47821 tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa
    47881 acacgatctt cccggtttga tgttgcgggc atttgatacc ttgccggaga tgatcatgac
    47941 gccacatcag gcatggcaga gacagattaa aggcgaagta gaaaccattg cgctggaaca
    48001 actggtcggt agagtatcgg caaatatgat cctgccttat ccacccggcg taccgctgct
    48061 gatgccggga gaaatgctta ccgaagagag ccgcacggta ctcgattttc tactgatgct
    48121 ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga
    48181 cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc
    48241 ttccgggcga gtaacgtgtt gttaacaaat aaaggagacg ttatgctggg tttaaaacag
    48301 gttcaccata ttgcgattat tgcgacggat tatgcgatga gcaaagcttt ctactgcgat
    48361 attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg
    48421 gatttggcgc ttaatgggca atatgtgatt gagcttttct catttccgtt cccgccggag
    48481 cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc
    48541 gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat
    48601 ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc gttggaactg
    48661 tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact
    48721 gtaaaatcgc accatcatga cactcacgct caatagacaa cttctctcct cacgccagat
    48781 tctggtggcc tttagcggcg gccttgattc tactgtctta ctgcatcaac tggtgcagtg
    48841 gcggacggaa aatccgggcg tcactctgcg cgctatccat gtgcatcacg gtttaagcgc
    48901 caatgctgat gcctgggtga aacattgtga aaacatctgc caacagtggc aggtgccgct
    48961 ggtggtcgaa cgtgtgcagc ttgcgcaaga ggggctaggt attgaggccc aggcgcggca
    49021 ggcacgttat caggcatttg cccgtacctt gttgcccggt gaagtgctgg ttacagcgca
    49081 acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc
    49141 cgggctttcg gctatggcgg aagtttcgga gtttgccgga acacggctta tccgcccgtt
    49201 gcttgcccgc acgcgggggg aactggagca gtgggcgcgt cagtatgatt tacgctggat
    49261 tgaagacgaa agtaatcaag acgattccta cgatcgtaac ttcttgcgcc tgcgcgtagt
    49321 gccgttattg cagcagcgtt ggccgcattt tgccgaagcg acggcccgca gcgccgcact
    49381 ttgcgccgaa caagagagcc tgctggatga actgctggcg gatgatttag cgcactgcca
    49441 aacgcaacag ggaacgttaa agattgcgcc aatgctggca atgagcgatg cccgtcgcgc
    49501 ggcgattatc cgccgctggc tggcagggca gaatgcacct atgccttccc gcgaggcgct
    49561 ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt
    49621 gggcgcgttt gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgttgccgg
    49681 gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat taccggcggg
    49741 gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc
    49801 ggtcagcgtg cgtttcaaag cgccaggact gctgcatatt gtcgggcgca acggtggacg
    49861 taagctgaag aaaatctggc aagagttagg cgttccgccg tggctacgtg acaccacgcc
    49921 actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg
    49981 tgtggctgta ggtgagaatg gcgtcagttt tgtctggcag aaaacgctta gtcaagtgaa
    50041 agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggac tcgctcacca
    50101 ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc agctcgcggg
    50161 ttgcaccagc ggcctcgacg accagatact ccacattttt gcgggagact aaatcgctgg
    50221 ccttcgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc
    50281 aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgta tcattcattg
    50341 acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca
    50401 gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc
    50461 ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa
    50521 tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc
    50581 tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc
    50641 gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg
    50701 cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt
    50761 ttctcgatgc ctctctgacg ttggcgcgcc atccttcaga aacccaggag cgtatgatgc
    50821 tgcgcctgct ggcgtggcta aaatatgccg atgaacgtct gcaatttacc cgtggtttgt
    50881 gtgccgatga tgagccggaa gcgtggctgc gtaacgatca tttgggtatt gatttgtgga
    50941 ttgagctggg gctgccggat gagcgacgga ttaagaaagc ctgcacccag gccgcagaag
    51001 tggcgctgtt tgcctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca
    51061 aatgtgcgca gtttgccaat ctttccgtct ggtatctgga cgacgagcag ctggcgaaag
    51121 tgagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct
    51181 ggttatcgga tgataagaat aatctggaag tgaacttaac cgtctggcaa caaccttcat
    51241 gattgtgatt tcccgacatg ttgctatccc tgatggcgag tttgagatca ccgccattcg
    51301 tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc atctgcgttt
    51361 tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca
    51421 tcatttgatc agcagtgacg gagtgattgt cattaaggca caggaatacc gcagtcagga
    51481 actgaaccgc gaagcggcgc tggcccggct ggtggcagtg attaaagatt taacaacaga
    51541 acaaaaagcc cgaagaccta cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc
    51601 gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtgcgcagcg gtcgggagta
    51661 aaaagaagga atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagccttt
    51721 ttactctgat gggatgtaat aatcgggccg aagtcgatac gctttccccg gcgcaggctg
    51781 ccgaactgaa accgatgccg caaagttggc gcggcgtgct gccgtgtgct gattgcgaag
    51841 gaatcgaaac ctctctgttc ctcgaaaaag atggaacctg ggtgatgaat gaacgttatc
    51901 tgggggcacg tgaagagcct tcctctttcg cttcctacgg tacatgggcg cgaactgccg
    51961 acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgagcg aaaggggatg
    52021 cactggagat gctcgatcgt gaaggtaatc ccattgaatc gcagttcaac tacacgctgg
    52081 aaccggcaaa atcgagcatg ccaatgacgc cgatgaccct gcgtggcatg tatttctata
    52141 tggctgatgc ggcgaccttt actgattgcg cgactggaaa acgttttatg gtggcgaata
    52201 acgcagagct ggagcgtggc tacctggctg cgcgcggcaa cggcgaaaaa ccggtgttac
    52261 tgtcagtaga aggtcacttt acgcttgagg ctaatccgga taccggtgcg ccgaccaaag
    52321 tattagcgcc cgatacggca ggtaaatttt acccgaacca ggattgcagt agtttggggc
    52381 agtaacccgt cttgaggcag aaacaaatga aatattgcct catgcgctgc gcttatcagg
    52441 cctaaagcat acaatgcaat attttgaatt agcacgattt tgtaggccgg ataaggcgtt
    52501 cacgccgcat ccggcatttt gcgcacgagg ctggctttta tcactgagtc agtccaacaa
    52561 agcgacttgc tttaatataa atccctggct tcaggtgccc aatatactgt aactgcgttt
    52621 ctggtttaaa tgcagagaca tcaccactac gcatatgcat cagatcggca gggctaatcc
    52681 agcctgattg cgccagcgta agcggatgcc cggctttggc aaatgcatcc gtgacaaatt
    52741 ccgaacaaaa ccacgacttt ttgtctcctt cgccaacgtt acttaactgc gctttcgcca
    52801 ggccgctgac gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc
    52861 gagtcaccat aaatggaata aattcgacaa tgccgcgata gttataaccg ctatctttaa
    52921 ttttattggc gaacgcggtg atttctgtgg cttgttgcgg ggtaaggtcc gggactcgta
    52981 agacgaaaag cttatcactg tgcttcatgg cttttttaag ggaaacaatc tggacgccag
    53041 cgcctgtcgc ttcggcaacg ttattatttc ccaaatagat tgcaacgtga ctcacagagg
    53101 aagtgctgaa tacgcggata ccaaatgagg ttaccccaag gcttgaggag aaaagcaaat
    53161 cgccgggttt gagatctggt tccgttattt cttttattga ttgttcggtg aaagagcttt
    53221 gatgctggaa tttaacagcc catgttttcg cctcggcatt taccgctgtg gctgatgagt
    53281 ctggctggct gatatcaacg gtacaggccg aaagtaaaag aaagcagggg agaagcaggc
    53341 ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg ccccgaaatt
    53401 caggaccctt tctggcatca gcctttaatc tgtttcacca gataatcgac gatgtcacca
    53461 gtcttaatta actgtttctc gccgttacga cgatatttat attcgatatc atcgttgtcg
    53521 aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac
    53581 atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcgcgcagt
    53641 tcgctgtaca gtttctcagc aagttcctgt acgcggaagg atttgtgcat gttcattggc
    53701 agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag
    53761 ttctgctcaa tcgccgctgc taccacacgc gttacgccga taccgtagca acccatcgtc
    53821 aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg
    53881 gtacccagct ggaagatgtg accgacttcg ataccacgtt tgatcagcag cgtaccctga
    53941 ccatccgggc ttggatcgcc agccaccacg ttgcggatat cagcaatttc cggggtagcg
    54001 acatcgcgat cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg
    54061 aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga
    54121 cccagtgaac ccggaccggc tttaaccacg gcacgaattt cttcttcggt cgcaaaagtc
    54181 agcgggctgg caacctgcgg cagtttttct gctttaactt cgttcagctc gtggtcaccg
    54241 cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa cggctttaac cagcagagtc
    54301 ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg
    54361 ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg
    54421 atagcttctg ccagttcaat gttcgctgcg tagtcagaag tatcggagaa gaccacatcg
    54481 tcttcaccgc tctgcgccag cacctgaaat tcgtgagagg cgctaccgcc gatagaaccg
    54541 gtatcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc
    54601 gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca
    54661 tctttcatca ggaattcgcg ggaacgcatg acgccaaaac gcggacgcac ttcgtcgcgg
    54721 aacttggtct ggatctgata gaagttcagc ggcagttgtt tgtaagagct cagctcgtta
    54781 cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca
    54841 cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac
    54901 aaatcggctg gctgaaccac cggcattaac acctcgatcg caccggcgtt gttcatctct
    54961 tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat
    55021 aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc
    55081 tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg
    55141 ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat
    55201 ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca
    55261 gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgcaacc
    55321 agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg
    55381 ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct
    55441 gcttttcgac ctctgcggta aaacaaaccg ccatttctgc cgctggcgca ctttgcgcat
    55501 agctggcact ggcatcggga agcgattcag caaaggggag atacggtttg atatccacta
    55561 ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg tctttatggc
    55621 aaacaacctc tttcagctct accagcgaca taccaatcgg gttggggcga aaagtggagc
    55681 gcgtagcgaa aacacccatt ctggcattac cgccgagacg aggcggacgc acagtcggac
    55741 gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt
    55801 ccaggccgcg aacggcgtcg gcctggttgt agggagcaat gagatgtagt tcgccgttgg
    55861 cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa
    55921 taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtgatgttaa gcgcagaacc
    55981 gatacataca gcctgacgat agcaacctgg cgtaccgctg gtgacttcgc agctatgcag
    56041 taataccgca ttggctttca ttttcgaggc gttgatttgc atccgcttac gtgcggttgg
    56101 aatgctcggc ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag
    56161 atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc
    56221 gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg
    56281 ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat
    56341 cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcaggc
    56401 gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa
    56461 acagcctaaa tattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc
    56521 gtaaacttcg tcagactgat aagcctgaac gaatttcttc acgttttcgg cgtctttgtt
    56581 gtcttcacgc gttacgatca ggtttacgta cggggactct ttatcttcga caaagatacc
    56641 gtctttcgct ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc
    56701 gatttgcgcg tcgtccagag agcgcggcag ctgcggtgct tccagttcaa caattttcag
    56761 attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag
    56821 tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaaggttag ttgggtcgtt
    56881 tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta
    56941 accagcaatc ggataaacaa atgtgttgcc tactgcgacc agtttgtagc cacgatcttt
    57001 cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct
    57061 caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata
    57121 tttgtctttc gcaactttct gcgcgacttc tgcaacctgc tgttcggcac caacaatcac
    57181 gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga
    57241 tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt
    57301 cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc
    57361 gcctgcgaac tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac
    57421 cgtcgcgttg tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc
    57481 gactgcacca cccatcgcgg aataaccgac cagggtaatc agggtaatag ttgccgcatt
    57541 caccagaccc ggcaacgctt ccggtaacag cactttacgg acgatctgca ttggcgtggc
    57601 acccattgcg cgggaggctt caattaaccc ggttgggatc tccagaagag cgttctcgac
    57661 catacgggca ataaacggtg ctgcaccaac ggttaacgga acaatcgctg cctgcaatcc
    57721 aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa
    57781 cgggatggaa cggaaaatgt tcacaatcgc agaaatggta cgatacaact tcgcgttagc
    57841 aataatttgc cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa
    57901 gccaaaaaaa ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag
    57961 ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc
    58021 aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc ataatgccga
    58081 acttaacgcc accggcgtaa tccatctgcg cgctaataat gttgttgttg acgttgaaac
    58141 gacgcgcggt ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca
    58201 gcatcggcac gcagtcagta aatggctccg cttgcagacg ttcctggtaa tcttccggga
    58261 tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga
    58321 acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat
    58381 cacaaatgcg cttcacaacg tccatttcgt gagtgatcaa cagaatcgtc aaacccagac
    58441 ggcggttgat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc
    58501 tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca
    58561 cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac
    58621 caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca
    58681 gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct
    58741 ggaaaatcat accaatctgg cgacgagctt tggtcaactc ggattctgac agcgtggtca
    58801 gttcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac
    58861 gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg
    58921 gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt
    58981 tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtggc
    59041 atttcgaatg cctggaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg
    59101 tctaaatcaa tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta
    59161 gcgtcacatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc
    59221 ttgaccgtga tggcaccatt aatgtcgatc acggttatgt ccatgagatc gacaactttg
    59281 aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg
    59341 tggtagtaac caaccagtct ggcattgctc gcggtaaatt tactgaagca cagtttgaaa
    59401 cgctgaccga gtggatggac tggtcgctgg cggaccgcga tgtcgatctg gatggtatct
    59461 attattgccc gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc tgcgactgcc
    59521 gcaaaccaca tccggggatg tttttgtcag cacgcgatta tttgcatatt gatatggccg
    59581 cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggctgcg gcgagcgtgg
    59641 gaacaaaagt gctggtgcgt acgggtaaac ctattacgcc tgaagcagaa aacgcggcgg
    59701 attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg
    59761 cgtaatgatt aaaagatgag cggttgaaat aaaaatgcat ttttccgctt gtcttcctga
    59821 gac
//
LOCUS       DAJOTB010000012        58246 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJOTB010000012 DAJOTB010000000
VERSION     DAJOTB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.012"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(42..323)
                     /locus_tag="OHN28_000757"
     CDS             complement(42..323)
                     /locus_tag="OHN28_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCQ2296199.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(586..1695)
                     /gene="apbC"
                     /locus_tag="OHN28_000758"
     CDS             complement(586..1695)
                     /gene="apbC"
                     /locus_tag="OHN28_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCQ2296200.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1827..3860
                     /gene="metG"
                     /locus_tag="OHN28_000759"
     CDS             1827..3860
                     /gene="metG"
                     /locus_tag="OHN28_000759"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCQ2296201.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4001..7801
                     /locus_tag="OHN28_000760"
     CDS             4001..7801
                     /locus_tag="OHN28_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCQ2296202.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPPMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            7814..11575
                     /locus_tag="OHN28_000761"
     CDS             7814..11575
                     /locus_tag="OHN28_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCQ2296203.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRACPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            11585..15217
                     /locus_tag="OHN28_000762"
     CDS             11585..15217
                     /locus_tag="OHN28_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCQ2296204.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15278..15594
                     /locus_tag="OHN28_000763"
                     /pseudo
     CDS             15278..15594
                     /locus_tag="OHN28_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16296..17384
                     /locus_tag="OHN28_000764"
     CDS             16296..17384
                     /locus_tag="OHN28_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCQ2296205.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17395..19674
                     /locus_tag="OHN28_000765"
     CDS             17395..19674
                     /locus_tag="OHN28_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296206.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            19667..20803
                     /locus_tag="OHN28_000766"
     CDS             19667..20803
                     /locus_tag="OHN28_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2296207.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRYSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20800..22803
                     /locus_tag="OHN28_000767"
     CDS             20800..22803
                     /locus_tag="OHN28_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCQ2296208.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22928..23389
                     /locus_tag="OHN28_000768"
     CDS             22928..23389
                     /locus_tag="OHN28_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCQ2296209.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23430..23900)
                     /locus_tag="OHN28_000769"
     CDS             complement(23430..23900)
                     /locus_tag="OHN28_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCQ2296210.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23947..24666)
                     /gene="yehT"
                     /locus_tag="OHN28_000770"
     CDS             complement(23947..24666)
                     /gene="yehT"
                     /locus_tag="OHN28_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCQ2296211.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24663..26348)
                     /gene="btsS"
                     /locus_tag="OHN28_000771"
     CDS             complement(24663..26348)
                     /gene="btsS"
                     /locus_tag="OHN28_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCQ2296212.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26570..27301
                     /gene="mlrA"
                     /locus_tag="OHN28_000772"
     CDS             26570..27301
                     /gene="mlrA"
                     /locus_tag="OHN28_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCQ2296213.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27361..27468
                     /locus_tag="OHN28_000773"
     CDS             27361..27468
                     /locus_tag="OHN28_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCQ2296214.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27449..28180)
                     /gene="yehW"
                     /locus_tag="OHN28_000774"
     CDS             complement(27449..28180)
                     /gene="yehW"
                     /locus_tag="OHN28_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCQ2296215.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28185..29111)
                     /gene="yehX"
                     /locus_tag="OHN28_000775"
     CDS             complement(28185..29111)
                     /gene="yehX"
                     /locus_tag="OHN28_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCQ2296216.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(29104..30261)
                     /gene="yehY"
                     /locus_tag="OHN28_000776"
     CDS             complement(29104..30261)
                     /gene="yehY"
                     /locus_tag="OHN28_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCQ2296217.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30268..31185)
                     /gene="osmF"
                     /locus_tag="OHN28_000777"
     CDS             complement(30268..31185)
                     /gene="osmF"
                     /locus_tag="OHN28_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCQ2296218.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31375..33672)
                     /gene="bglX"
                     /locus_tag="OHN28_000778"
     CDS             complement(31375..33672)
                     /gene="bglX"
                     /locus_tag="OHN28_000778"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCQ2296219.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            33868..35583
                     /gene="dld"
                     /locus_tag="OHN28_000779"
     CDS             33868..35583
                     /gene="dld"
                     /locus_tag="OHN28_000779"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2296220.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35622..36554)
                     /gene="pbpG"
                     /locus_tag="OHN28_000780"
     CDS             complement(35622..36554)
                     /gene="pbpG"
                     /locus_tag="OHN28_000780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCQ2296221.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36728..37315)
                     /locus_tag="OHN28_000781"
     CDS             complement(36728..37315)
                     /locus_tag="OHN28_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCQ2296222.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37485..38063
                     /locus_tag="OHN28_000782"
     CDS             37485..38063
                     /locus_tag="OHN28_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2296223.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38193..38954)
                     /locus_tag="OHN28_000783"
     CDS             complement(38193..38954)
                     /locus_tag="OHN28_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2296224.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(39007..40443)
                     /gene="mdtQ"
                     /locus_tag="OHN28_000784"
     CDS             complement(39007..40443)
                     /gene="mdtQ"
                     /locus_tag="OHN28_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCQ2296225.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40667..40750
                     /gene="yohP"
                     /locus_tag="OHN28_000785"
     CDS             40667..40750
                     /gene="yohP"
                     /locus_tag="OHN28_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCQ2296226.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41138..42088)
                     /gene="dusC"
                     /locus_tag="OHN28_000786"
     CDS             complement(41138..42088)
                     /gene="dusC"
                     /locus_tag="OHN28_000786"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCQ2296227.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            42327..42725
                     /locus_tag="OHN28_000787"
     CDS             42327..42725
                     /locus_tag="OHN28_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCQ2296228.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42722..43417
                     /locus_tag="OHN28_000788"
     CDS             42722..43417
                     /locus_tag="OHN28_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCQ2296229.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43547..44431
                     /gene="cdd"
                     /locus_tag="OHN28_000789"
     CDS             43547..44431
                     /gene="cdd"
                     /locus_tag="OHN28_000789"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCQ2296230.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44581..45300
                     /gene="sanA"
                     /locus_tag="OHN28_000790"
     CDS             44581..45300
                     /gene="sanA"
                     /locus_tag="OHN28_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCQ2296231.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45303..45542
                     /locus_tag="OHN28_000791"
     CDS             45303..45542
                     /locus_tag="OHN28_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCQ2296232.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            45736..46974
                     /gene="preT"
                     /locus_tag="OHN28_000792"
     CDS             45736..46974
                     /gene="preT"
                     /locus_tag="OHN28_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCQ2296233.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            46968..48203
                     /gene="preA"
                     /locus_tag="OHN28_000793"
     CDS             46968..48203
                     /gene="preA"
                     /locus_tag="OHN28_000793"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCQ2296234.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(48446..49456)
                     /gene="mglC"
                     /locus_tag="OHN28_000794"
     CDS             complement(48446..49456)
                     /gene="mglC"
                     /locus_tag="OHN28_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCQ2296235.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(49472..50992)
                     /gene="mglA"
                     /locus_tag="OHN28_000795"
     CDS             complement(49472..50992)
                     /gene="mglA"
                     /locus_tag="OHN28_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCQ2296236.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(51053..52051)
                     /gene="mglB"
                     /locus_tag="OHN28_000796"
     CDS             complement(51053..52051)
                     /gene="mglB"
                     /locus_tag="OHN28_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCQ2296237.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(52331..53371)
                     /gene="galS"
                     /locus_tag="OHN28_000797"
     CDS             complement(52331..53371)
                     /gene="galS"
                     /locus_tag="OHN28_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCQ2296238.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(53513..54670)
                     /locus_tag="OHN28_000798"
     CDS             complement(53513..54670)
                     /locus_tag="OHN28_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCQ2296239.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(54687..55355)
                     /gene="folE"
                     /locus_tag="OHN28_000799"
     CDS             complement(54687..55355)
                     /gene="folE"
                     /locus_tag="OHN28_000799"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCQ2296240.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            55613..56449
                     /gene="yeiG"
                     /locus_tag="OHN28_000800"
     CDS             55613..56449
                     /gene="yeiG"
                     /locus_tag="OHN28_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2296241.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(56481..>58246)
                     /gene="cirA"
                     /locus_tag="OHN28_000801"
     CDS             complement(56481..>58246)
                     /gene="cirA"
                     /locus_tag="OHN28_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCQ2296242.1"
                     /translation="VQLTNEGDNRKGVSIRGLDSSYTLILVDGKRVNSRNAVFRHNDF
                     DLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKIGQKWSGTVTVDTTVQEHR
                     DRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNSTTTDTGETPRIEGFSSRD
                     GNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQNYSVSHNGRWDYGTSELKY
                     YGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTVGGEWRHDKLSDAVNLTGG
                     TSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGEHWSPRAYLVYNATDTVTV
                     KGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPETSESWELGLYYMGEEGWL
                     EGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFETGANGRRIPVFSYYNVNKA
                     RIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLSDLPFHTANGTLDWKPLAL
                     EDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVKLRAGVLNLGDKDLS
                     RDDYSYNEDVRRYFMAVDYRF"
BASE COUNT        14384 a        15315 c        14594 g        13953 t
ORIGIN      
        1 gatttaagct ttctaaatcc gattaaaacc agggtggcaa attaatcgta gcgatttaac
       61 acgattcgac gtttgctatc gctatctgca gcaatcactt ccgtcgttac acctttaact
      121 ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta actgaccaca atgctgttga
      181 ggttgaccat taaccatagc catactgcat gtcgggggac tgatcctacc attaatcgtt
      241 aatgtggcag tttctgaaga gaacgcggga gaagccaatc cataggcaag aaaaataatt
      301 cctgacagcc agtacttatt cattgaacgt tatccctgta gtaaaggtta tgcctggaaa
      361 gaatgagtgc ataacaaact atagctgtac atccactaca cagccacgaa ggatgataat
      421 gaagcatcgc cggtacgatc aatcgacttt gtagagtttc ggacgaaggt ccgcagaata
      481 ttcgcaacat taaataagtg ttcattaatg ttgcaaaaac aaatacaccg catcaatgta
      541 acatctctgc ggtgtatgaa gaataaccag aatggttaat acgcgttaga ccgcgcggaa
      601 ggaaatctcg ccaggaatga cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc
      661 cagctggcgg tagatagcgg taaattcgct ctcaggacgg ctgataaccg ttggcgtgcc
      721 tttatcgaga tcctcacgta aggagatgtg taacggcatc tgccccagca gctgcgtgtg
      781 gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa atcggctcat ggtgaccaca
      841 gttactgcaa atatgcacac tcatgttctc gacaataccg agcaccggaa cctcgacttt
      901 ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac
      961 cactaccgcg ccagttactg gaatgttttg tgccagtgtc aattgaatgt caccggtacc
     1021 tggcggcata tcaagcacca gatagtcgag atccggccac aaggtttcct gcaacatttg
     1081 cattaacgcc ttgctcgcca ttgggccacg ccacaccatc gcattgtcgt cagtgaccag
     1141 ataaccaatt gaattggttg ccaggccatg agacataatc ggtgccatat gggtaccgtc
     1201 cggcgaagtt ggacgctgat tttctgcgcc cagcatggta gggattgatg ggccgtagat
     1261 atcggcgtcc agaatgccga ctttcgcccc ttctgccgcc agtgccaacg ccaggtttac
     1321 cgccgtggag gatttaccca cgccaccttt accggaactg accgcgataa tgttcttcac
     1381 gccgttaatt cccggctgat tcttcacgcg tttcagagtg gctatgttat gcgacagttt
     1441 ccagtcgata gctttcgctc cggtgatgcg cagcagttcg gcgctacatt gctcttttaa
     1501 ctcttcgaac gcgctatgcc agacgaatgg catgaccagt tccacatgca gcgtgtcgtc
     1561 catccaggcg acatggtgca acgctttcag cgttgtgagg ttatgcttca gggttgggtg
     1621 ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg atttggcctg
     1681 ggattgttcg ttcatcccga ctcctttttt gtatagataa accatcagct gatagtttac
     1741 ctgaagaata tagagaagta cttacttaac attttcccat ttggtactat ctaagccctt
     1801 ttcactatta agaagtaatg cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt
     1861 gcgcactgcc gtacgctaac ggctcaatcc acctcggcca tatgctggag cacatccagg
     1921 ctgatgtctg ggtccgttac cagcgaatgc gcggccacga ggtcaacttc atctgcgccg
     1981 acgatgccca cggcacgccg atcatgctta aagctcagca gcttggtatc accccggagc
     2041 agatgattgg cgaaatgagt caggaacatc agactgattt cgcaggcttt aacatcagct
     2101 atgacaacta tcactcgacg cacagcgaag agaatcgcca gttgtcagaa cttatctact
     2161 ctcgtctgaa agaaaacggt tttattaaaa accgcaccat ctctcagctg tacgatccgg
     2221 aaaaaggcat gttcctgccg gaccgttttg taaaaggcac ctgcccgaaa tgtaaatccc
     2281 cggatcaata cggcgataac tgcgaagtct gcggcgcgac ctacagcccg actgaactga
     2341 tcgagccgaa atcggtggtt tctggtgcta ccccggtaat gcgtgattct gaacacttct
     2401 tctttgatct gccctctttc agcgaaatgc tgcaggcatg gacccgcagc ggtgcgttgc
     2461 aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc tggcctgcaa cagtgggata
     2521 tctcccgcga cgcgccttat ttcggttttg aaattccgaa cgcgccgggc aaatatttct
     2581 atgtctggct ggacgcaccg attggctata tgggctcttt caagaatctg tgtgacaagc
     2641 gcggcgacag cgtaagcttc gatgaatact ggaagaaaga ttccactgcc gagctgtatc
     2701 acttcatcgg taaagatatc gtttacttcc acagcctgtt ctggcctgcc atgctggaag
     2761 gcagcaactt ccgcaagccg accaacctgt ttgttcacgg ctatgtgacg gtgaacggcg
     2821 cgaaaatgtc caagtctcgc ggcaccttta ttaaagccag cacctggctg aatcattttg
     2881 acgccgacag cctgcgctac tactacaccg ccaaactgtc atcgcgcatt gatgatatcg
     2941 atctcaacct ggaagatttc gttcagcgcg tgaatgccga tatcgtgaac aaagtggtta
     3001 acctggcgtc ccgtaacgct ggctttatca ataagcgttt tgacggtgtg ctggcaagcg
     3061 agctggctga cccgcagttg tacaaaacct tcactgatgc cgctgaagtg attggcgaag
     3121 cgtgggaaag ccgtgaattt ggtaaagcta tccgtgaaat catggcgctg gctgacctgg
     3181 ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc gaagcaggaa ggtcgcgatg
     3241 ccgatctgca ggcgatttgc tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc
     3301 tgaaaccggt actgccgaaa ctgaccgagc gcgcagaagc attcctcaat acagaactga
     3361 cctgggatgg tatccagcaa ccgctgttgg gccataaagt gaatccattc aaggcgctgt
     3421 ataaccgcat cgatatgaaa caggttgaag cactggtgga agcatccaaa gaggaagtaa
     3481 aagccgctgc cgcgccagta actggcccac tggcagatga tccgattcag gaaaccatca
     3541 cctttgacga ctttgctaaa gttgacctgc gcgtggcgct gattgaaaac gcagagtttg
     3601 ttgaaggttc tgacaaactg ctgcgcctga cgctggatct cggcggtgaa aaacgcaatg
     3661 tcttctccgg cattcgttct gcttacccgg atccgcaagc actaattggt cgtcacacca
     3721 ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg tatctccgaa ggcatggtaa
     3781 tggctgctgg tcctggtggg aaagatatct tcctgctaag cccggatgcc ggtgctaaac
     3841 cgggtcatca ggtgaaataa tcccccttca aggcgctgca tcgacagcgc cttttcttta
     3901 taaattccta aagttgttct cttgcgattt tgtctctctc taacccgcat aaatactggt
     3961 agcatctgca ttcaactgga taaaattaca gggatgcaga atgagacact ttatctatca
     4021 ggacgaaaaa tcacataaat tctgggcggt ggagcagcaa ggcaacgagt tacatgtcag
     4081 ttggggcaaa gtcggcacta acgggcaaaa ccaggtaaaa agctttgctg atgctgcggc
     4141 ggcggaaaaa gcaaagctta agctgatcgc agagaaaacg aaaaaaggct atgtggaaaa
     4201 tgcttctgca aacgtgcata tcacccccat caccaaagcc actcctgaag ttgaaacttc
     4261 tcctgagagt aaaaaccaac gcccctggct ggctgatgat gcggtcattc ggctaactga
     4321 cgatattaat cgatttgctt ttcctcatcg ctctcgcccc agagagatca attatctgca
     4381 caaagatagt caaatatggc agcgcatcgc gaataacacc agggcatatg atcccgccaa
     4441 caattaccgt tcttatccag aaaactggca gcaggctttt gctgagttac aaaggcgaat
     4501 tctggataat caacagacag ggagtgcgca atctgatgcg gcattgctct ggagtttctg
     4561 gaattcttac tctgcagatg aactagtcga tgacttaatc attcgctgtg gtatggaaag
     4621 tgccgttgaa attgcccttc tcgctcttca actgagatat aaaccagtaa aaggcgcagt
     4681 aaccaccatc attccaccta atcacgaagc agaatcgcta ccgagttggc atcaacgcct
     4741 atgtcatcat ctttcactcg cttcagaaga tgagtggcaa cgctgtgtag ataaagtact
     4801 cgcggctatt acgacgctat ccccagcacg gcaacctttt gcggcgttac tgctccctga
     4861 acgcccggat attgccaatg ctatggctct acgctatgca gatcaaaacg ttccggcgat
     4921 gacctggtta agactggtag taaccgatga agccgctctg actgcagccg aaaaatacga
     4981 ctttccaccg ttatatcgtg attttcgcgc ttatctggca atgttgctgg caaataatgg
     5041 ggtgagcggt gtaagccgga tacttactga gtttgtggaa gagcattctg acaatcccac
     5101 ttatctcttc gagcgcatca gcgagactga agatcttgtc aaatggttat ggaaaacgaa
     5161 tcacccggat gcgattcaaa ttctgatcct cggtgtaatt ggcaagaaaa agcacctgga
     5221 atacttaagc aaagcatgtc aaaaacatcc cgctgcggct attgccgctt atgcaacttt
     5281 gctggcaata catgaagata aagagtggca taaagcgctt gtcaaattga ttaccatcac
     5341 gccagagtta gtatgcgacg tcattccctg ggttagtgct aaagcggcag gcatcttatc
     5401 tgaatgtcgt cctcagtcag tagctgaaga atgtgagtat gccactgcgg atatgttgcc
     5461 agagctgttt gtttcgccgc cctggatgac taaagagaaa aagaaaaatt cgccggtgtt
     5521 tgatcttcct gtactgccag ttccttctgt ttctgacgtc actccagaga ttgccaaaaa
     5581 actaacccgt acataccttg tcacccgctt ccagcaaata gcccaacagc aggcaacaaa
     5641 acaaatattg tttaccgacc tgccgccgat ggaaaaagca agctgggaga gacacttagt
     5701 ccccctcaca ccagaacagc aaattctgtg gtgtctgggt tttgacaaat ggcgggagag
     5761 cggagaaaaa atatacgaaa aaattccggc gccacaaagt gccgttgatg ccctgctacg
     5821 cttcgatttt ccagcactca atgctgaatt tgcgcaatac cataacaatg cttacaaaag
     5881 ctggaatctc attgccctct gttatctacc tggtcaacag gccatttctt ttcttaacca
     5941 gatagtcaaa gaagataact actctggtga gggatatatt ctggccattt ttggtagcgc
     6001 cgccattcct gcatttatga cctgcctgca acgtgagcca caacggttgt ggtttttcac
     6061 cttcttcctt ggtgtcaatg aactggcatt gccaatggcg caacgacttc agaaaaaaat
     6121 gtcgtatgaa gatgcacgaa actggctaac ggattaccca cgccatgccg ccgccgggct
     6181 acttcctgtg gcactgggca aacaaggaaa agatcgtgat tgtgcccgcc aggcgctccg
     6241 tctgctggtg aatttaaacc agcgagaaac gattgaggaa atcgcccaag gatacaacca
     6301 acctgatgtt ttagctgcgc tggcaacatt atttgatagc gagccgctgg aagaatatcc
     6361 cgctaaaatc gccccactgc ccggctttta tcagttcacc ttatggcgca gaccacggct
     6421 taaaagtaac aaccagcctc tgccagatga cgctatgcgc cacctcggca taatgctgag
     6481 ctttccacgc gacattaccc cctacgcagg gcttgcgatc atcaaagaaa ccttcacccg
     6541 cgaatcactg gctgaatttg gctgggatct gtataccgcc tggacagaag ctggcgcacc
     6601 cgcaaaagaa aactgggcat ttacctcgct ggggatttta ggcaatgacg acaccgcgcg
     6661 taaattaacc ccgctcatcc gcacctggcc gggtgaatcc cagcataaac gcgcggttta
     6721 cgggctggat gtgttagcca gtatcggtag cgacattgcg ctaatgttgc ttaatggcat
     6781 tgcgaaaaag attaaattcg ttgcattaca ggaacatgcc tgcgacaaaa tcaacatggt
     6841 cgctgaaaat cgcgggctga ctatggctga actggaagac cgcctggcac cggatttagg
     6901 acttgatagc agtggctcgt tgacactgga tttcggccca cgtcaattta ccgttggttt
     6961 tgacgaaact ttaaaacccg tggttcgcga cgcaaacggc aaagttctga aagatttacc
     7021 caaaccaaat cagagcgatg ataaaacgct ggcaactgac gcggttaatc tcttcaaaca
     7081 gttgaaaaaa gatgtacgcg ccatagccag ccagcagatt gatcgtctgg agcaggctat
     7141 gtgccagcgc agacgctgga cggcagagca gttccgcctg ttcctggtgg agcatccgct
     7201 ggtacgtcac ttaacccgac ggttgttatg gggggtatat actgaggaaa acacgctgat
     7261 tgcctgcttc cgggtcgcag aagacagcac ttacagcgat gcgcaggatg agctatttac
     7321 gttgccagca ggaaacatcg gtattccgca tgtgctggaa atatctcctg aatccgccgc
     7381 tgcatttggg caaatttata ctgattacga acagcttcct ccgttccgcc aactcgatcg
     7441 tggttattac caccttgctg ataatgaacg tgattcccat gaattgatac gctggcaagg
     7501 acgtctttgc caggccgggc gcattgtcgg gctggaacgc cgaggctggc aacgtctgga
     7561 agaaagcggc agcgtttacg cgatgcgcaa atctactcct tatggcgatc tcgaacttga
     7621 aacagaacct ttttcattaa tttatggtga aacggggtat ggcgaccagc taccggttga
     7681 aagcgtaaaa atcacctcgc ctgataatcg ctacggtaaa caatcctcac tcactttttc
     7741 cgcgctcgac gacatcaccg ccagcgaact cattaacgac attgaatcac tgtttgatta
     7801 aaaggattta gcaatgaaaa cctatattta tcaggacgaa aaatcacata aattctgggc
     7861 agtagagcag caggaaaatg aactgcatat cagttggggc aaagtcggca ctaacgggca
     7921 aagccagata aaaagctttg ctgatgcggc ggcggcggaa aaagcggagc tgaagttgat
     7981 tgcggagaaa acgaaaaagg gctacgcaga agaagtatcc gtgacgacac cgacatctgc
     8041 ccccgtccag gttatcgcat gctctgaaaa agcccctttg ccgcaagaca aaccaccatt
     8101 tgctgaagac caccttccct ggctggcaga cgacgcccaa atcattttac cgacagaggt
     8161 cgcaccaacc acactaagtc atcgacgctg gcctggcgat tctgtgccac aggagaatga
     8221 attaactctg ctacgctccg tcgccgcgca tactcaccgg cgttttaaaa aggtgatcac
     8281 cttcgattac agcacctgtt cactggactg gcagcaggcg attacacaag cagtgggcct
     8341 tatcgactca ccgattagca ccgcactacc accgatggtt ctggcggtac tggttgcgct
     8401 ggaacaaggc ttcaatcgca acgatcacga agagttaatg gatcaaattg tccaggaggg
     8461 tggtctggaa tatgccactg aggttgtcat tgcattgcaa gccatccgct ttgactggaa
     8521 ttatgatgcc cacctcatta cgtttacccc ggataacaag cagcctggct atctgtcacg
     8581 ctttgcctcg gttgaaatgc gtctgcgtaa acatctctca ctagcaaatg acgatgtatg
     8641 gcagcgctgc gccgacaaac tgattgcagc tctcggaaac atacccgcct ggcatcagcc
     8701 gctggttgcg ttattgttgc cggaaaaaca agatgtgagc catgagattg ccagacgtct
     8761 ttgcggacaa aaagggttat atgcgctgga atggctaaaa ctaacggccg ccgacgagca
     8821 ggtattggca gaactgggga aatactatcc aggacagcca gggcaggtat ttgatgatta
     8881 ttacggcggt aaaatctggt gcgcaacgat actcaaggag caaggtgtcg gtgcgctggc
     8941 ccgttttgct ccttacgctg ctggtgacac atgtggtgaa gtgttgatgc atatcaacca
     9001 cccgcaggca cttacactac ttatccatgc aagcgagcag ggaaagcgtt gccacgatcg
     9061 catgacaaag actttcgttc gctttccaca tgcagcgctg gcggcattag cagaacttct
     9121 ggcacaaaaa gatcaaaagc gttggcgcat gatgctaatg acaatgctta tctcacaacc
     9181 aacattagca gagcgagtaa tcccctggct ctcaacaccc gcagttgccg tactgaaatc
     9241 atgccagcaa caactgacac agccctcaaa ccatgccagc gctgatatgc tgcctgccgt
     9301 attagtctca ccgccctggc tttcgaaaaa gaaaaaatct gtaatgccgg tattggattt
     9361 aacgccatta ccgctggagt catgctgcac attgaccgag acggcagaaa aagagattca
     9421 tgcaaggcat cgctggcacg cacatcaaat agacatcggc caaaaagagg atattcagaa
     9481 ctacctgaca cgtctgggtt tcaatcgctg gaataatggc cagtatatga aagcgtctga
     9541 tgctgttgta gaactctggc agaggggaga ttattctgcg ctaatcagcg aatttaaaac
     9601 cttctggcac tcctatcaac gggaatggca gctttacatg ttagccgccc tgcctatcga
     9661 aaaaacagcg caggcgtgga atgtcctcag taaagagcca cacgtcggtg tcgagtttgt
     9721 catgacgcac ctgcaactcg ctgggttaca gggttttatt cactcctttt cacgctatcc
     9781 acaagaagct ctgcctgtag cacaatattt tgcggctatc gaattagcgc cactgatcgc
     9841 ccgcgccttc aacaaactca aaacattacg acaagacgcc cgtatctggc tgctgaaata
     9901 cccggaacac gccattaccg gcctgctgcc tgcagcgctc ggcaaaaccg gtgaagcaca
     9961 ggacaacgcc cgtgctgcct tgcgtatgct taccgaaaac ggtcatcagc cattgctgca
    10021 agaaatcgcc cggcgttata accagccgga agtaaccgat gcggtgaacg ctctacttgc
    10081 gctcgatccc ttagataatc acccgacaaa aatccccact cttccgacct tttatcagcc
    10141 atcgctctgg acgcgcccgt tattaaaagc aaacgcccaa tcactgcctg atagcgcact
    10201 cctccacctc ggtgaaatgc tccgcttccc tcaggaagag gcactgtatc caggattatt
    10261 gcaggtgaaa gatgcctgta ccaccgactc actggctgaa tttgcctggg atctgtttac
    10321 cgcctggcag accgctggcg cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt
    10381 tctcggtaac gatgacaccg cccgcaaact gacgccatta atccgtgcct gccctggcga
    10441 atcccagcat aaacgtgcca ccgttgggtt ggatatcctc gccgctatcg gtagtgatat
    10501 cgccctgatg cagcttaacg gcatcgccca gaaactgaaa ttcaaagcat tacaggagcg
    10561 ggcaaaagaa aaaatagcca acattgccga gagccgcgaa ctcacggtgg cggagcttga
    10621 agatcggtta gcaccggatc tcggtctgga tgataacggt tcgttgctgc tggatttcgg
    10681 cccacgccag ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc gtgacgcttc
    10741 cggcagtcgt ctgaaagacc tgcccaaacc aaacaaaagc gatgatgaat cacaggcgaa
    10801 caatgctgtt aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg ccgcccagca
    10861 ggtagcaagg ctggaatccg ccatgtgcct gcgtcgccgc tggtcaccgg aaaactttca
    10921 gctcttcctg gttgagcatc cgctggtccg ccacttaacc cgccgtctga tttggggcgt
    10981 ttatagcgcc gaaaaccagc tactggcttg ctttcgcgta gcggaagata acagctacag
    11041 caccgctgac gatgatcttt tcaccctgcc ggaaggcgat atctctattg gtattcctca
    11101 cgttctggaa atatcaccga cggatgctgc cgccttcggg cagctttttg ccgactacga
    11161 actgttacca ccgttccgcc agcttgatcg taacagttac gcgctgacag aagccgagcg
    11221 caatgccagc gaactgacac gctgggcagg cagaaaatgc ccgagtggtc gggtaatggg
    11281 gctggcaaat aaaggctgga tgcgtggtga accgcaggac ggtggctgga tcggctggat
    11341 gatcaaacct ctgggccgct ggtcgttaat catggaaatc gatgaaggtt ttgcggtcgg
    11401 catgtcgcca gccgaactca gtgccgagca actcttaagc aagctgtggc tatgggaagg
    11461 caaagcggaa agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact
    11521 cgatgccatc accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa
    11581 gagcatggac aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa
    11641 aaaaggcaaa acaccactca gccatcgcaa ttggccaggc gaaccggtac ctgttatcac
    11701 tgaaaacatc attcagacac tgggtgatga attgctacaa aaagcagaga agaaaaaaaa
    11761 cattgtctgg cgttatgaaa acttttcact ggagtggcag tccgccatca cgcaggccat
    11821 caacttgatc ggcgaacaca aaccctcaat cacggcccgg acaatggcgg cgctagcctg
    11881 tatcgcgcaa aatgatagcc aacagttgct cgacgaaatc gtccaacaag aggggctgga
    11941 atatgcaact gaggtggtga ttgcacgcca gtttattgta cggtgttatg agagtgatcc
    12001 tctggtagta acattgcaat atcagaacga ggactatggc tatggttatc gctcagaaac
    12061 ctataacgaa ttcgatctcc gattgcgtaa gcatctctct ctggcagagg aaagctgctg
    12121 gcagcgttgc gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc
    12181 ttttattgcg ctgaacctcc cggaaaaacc cgaaatcgcc aacgagctgg taagccttga
    12241 atgttcgcaa actcattttc gctcaaaaga gtggttaaaa gttgttgctg atgaccctaa
    12301 ggcggtgaaa gaactcgcac gctactggag ccaggatata tttagcgatc gagaagccag
    12361 ctacatgtcg catgaaaacc acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca
    12421 aggactggca gccgttccgc gcctcgcgat gtatgcacat aaagaagatt gcggcagtct
    12481 gctggtacaa attaaccatc cgcaagtcat ccgcaccttg ctactggtgg ctgataaaaa
    12541 caaacccagc ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacgctcgc
    12601 cgcactggca gagctgctgg cgttaaaagc cccccctgcg cgccctggtt atcccatcat
    12661 cgaagacaaa aagctgcctg cacagcaaaa agcacgtgat gaatactggc atacgctgtt
    12721 acaaacgctg atggcatcgc agccacaact ggcagaagag gtgatgccgt gcttatgtac
    12781 acaagcccag gcagtggtga atggttattt atcggcatcg ccaaaacttg ctttcgagag
    12841 tactcatagc aacgacctgc cagaaatact tgtttctcca ccgtggcgag gtaagaaaaa
    12901 aacggcattg ctgcgccttg atttagtgcc tcttgaatta gcgcccaaag cccgctggca
    12961 accaggcgaa cgagagagac ttgccgccac agagtctgcc cgttatttca gcacgggatc
    13021 ttttacggaa cgcatggaac gaaaaagtgg tcgagttgta ctacaggagc taggttttgg
    13081 ggatgatgta tggctgttcc ggaattatat actgcccggc aaactggatg cggcgcgcaa
    13141 atcattagtg ggtcagtggc attactcccc gcgcagagta gaggaaatca acaatggctg
    13201 gcactccacg gaagcaaaat cagccgaaca ggcgcttcgt tccggcgacg ttgaagcgtt
    13261 aattaacacc tgggaaaatg atagctactc gcattatcgt caggaaaaga gtgtctggaa
    13321 cctgtattta ttggcgcagt tgccacgtga gatggctttg actttctggc tgcgtatcaa
    13381 tgagaaaaag catctattcg cgggtgagga ctattttctc agtatcctcg ggttggatac
    13441 attacctggc ctgttattgg ctttttcaca tcatccaaaa gaaacatttc cgttaatttt
    13501 aaatttcggc gcaacagaac tggccctgcc cgttgcccgc gtctggcgac gtttcgcggc
    13561 gcagcgtgat ctggctcgcc agtggatttt acaatggccg gaacatacgg ctactgcact
    13621 tatccctctt gtctttacaa aacccagcga taatagcgaa gctgcattac ttgccctgcg
    13681 tttactgtat gaacaagggc atggcgaatt actgcaaacc gtggcaaacc gctggcagcg
    13741 tacagatgta tggcctgccc tggagcaatt acttaaactg ggtccaatag aaatttaccc
    13801 ggcacgtatt ccaaaagctc ctgatttctg gcatccggga atgtggtcca ggccgcgcct
    13861 tatcactaat aatcagcctg ttaccgatga cgctctggaa attatcggcg aaatgctgcg
    13921 ctttacccag ggcggacgtt tttatggcgg actggaacag ctgaaaacgt tttgccagcc
    13981 gcaaacgctg gcagcttttg cctgggatct tttcaccgcg tggcaacaag ctggtgcccc
    14041 cgcaaaagac aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa ccacggcacg
    14101 agatctgacg acacagatcc tcgcctggcc acaagaaggc aaatctgccc gtgctgtcag
    14161 tggcctgaac atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcaccatat
    14221 atcccaacgg gcgaaatccc gctccttacg tgataacgcg gcggaatttc ttcaggtggt
    14281 cgcagaaaat cgcgggctaa gccaggaaga gttagcggac agattagtcc caacattggg
    14341 ccttgatgat ccgcaggcgt tgagttttga ttttggtccc cggcagttta ccgttcgctt
    14401 cgatgaaaac ctcaacccgg ttatctttga tcagcaaaac gttcgccaga aaagtgttcc
    14461 ccggttgcgc gccgatgacg atcaactgaa agcgcccgag gcactggccc gactaaaagg
    14521 gctaaaaaaa gatgccactc aggtgagcaa aaacctgctc ccacgtcttg aagcagccct
    14581 acgtatcacc cgccgctggt cgctggcaga ttttcattct ctgtttgtta atcatccctt
    14641 tacccgcctg gttacccagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg
    14701 tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcac aagatgagcc
    14761 cattgacctg cctgcggatg ctctgattgg cattgcccac ccgttagaaa tggcagcaga
    14821 aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca
    14881 gttggcgcgg cgcacggtgc tgctcacacc tgacgagtca gccagtaaca gcctgactcg
    14941 ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga
    15001 gtcaggctat gaggacgcat ttgtctatga cttaggtgcg taccggctgg tccttaagtt
    15061 ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct tccgttctct
    15121 tcgggtttac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag
    15181 tgaggcatta agcgcacctg acgtcatttt ccattaaaac acagcgggca gtgatgcaac
    15241 tgcccgttat caacacgacc taccagcggg ggaagcgatg atcgtgcaaa aagagctggt
    15301 tgctatttac gattatgaga tccctgtacc tgaagagccg ttttccttca gacttgaaat
    15361 ccataaatgc tctgaattat ttacaggttc cgtctatcga ctggagcgat tccggctaca
    15421 tccaacattt catcaacgtg atcgagaaga tgccgaccac taataaatga tgcgttgatt
    15481 tatataagag atgagtttat tgatgagcgg aaattacgag gtgaatcaca tgaaacagta
    15541 atagcaattt ttaattgtga actacagaat atattcaacc aagaaataga ataatatact
    15601 ctagataatt caagtcgcag gaaggcgata agcgagtgag tccccaggag cttactaaag
    15661 taagtgacag gggtgaacga acgcagtcgc agcacatgca acttgaagta tggcgagtat
    15721 agctttatta aagggggaaa cggtattccc ccttagatgc gtttcaattt gcccgttact
    15781 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
    15841 ttaacttttg tgtcagcgtg tccatccgct ctccttttaa ggagcgcaac accagcacct
    15901 tacccttaca ctgcctgtcg atactggttt acgtatgttg gaccacgatg ttgcactccg
    15961 gatcaggcac cacctgcagc accacccgtc cggtagccag gacttgaaat tggttcgatc
    16021 cggcgcaacc tcccaacagt caggattata aatctcatta tcttaattgc aacggggtcc
    16081 agcggtggtt ataattcgtt cgttggaaca cgatgttgca caggctgcgg tgttaggcct
    16141 gaaagtagta aataaaagaa cctgcctcac cagcaggttt ttttattttt tgcgatctgc
    16201 tttccagata ttttccgctc aaacaactaa tgcgccaaac atttattgcg cgtaaaatat
    16261 cgtttatttc attaatacat ttcagggatg aatatatgtc accgcagaat aatcacctgc
    16321 agcgtccgcc tgctgctgtg ttatacgccg ttgaactggc aaaattaaaa caaaatgata
    16381 acgcaccttg cccgcccggt tggcagttaa gtttgcctgc ggcccgtgct tttattcttg
    16441 gcgacgaagc gcaaaatatt cgccgtaaag tggtgattag tccctctgct gtcgaacgca
    16501 tgttagtcac tcttgccacc gggcgcggtt tgatgttggt gggggaaccg ggtaccgcaa
    16561 aatctctcct gtctgaatta ctggcaaccg ctattagcgg cgatgccggg ttaaccatcc
    16621 agggcggcgc atctaccacc gaagatcaaa tcaagtacgg ctggaactac gccctgctta
    16681 tcaatcatgg tccgtcaacg gaagcgctgg tccctgcccc gctttacctg ggaatgcgcg
    16741 acggcaaaat cgtccgtttt gaagagataa cgcgtactcc actggaagta caagactgtt
    16801 tattaggtat gctttctgac agggttatga cggtgccgga actcactggt gaagccagtc
    16861 aactctatgc tcgggaaggc tttaatatta ttgccactgc caatacgcgc gatcgcggcg
    16921 tcaatgaaat gagtgccgcg ctaaagcgtc gcttcgattt tgaaactgtc tttccgatta
    16981 tggatttcgc ccaggagctg gagcttgtcg ccagtgcttc ggcgcgtttg ctggcccata
    17041 gcggcattcc gcataaagtg cctgacgcgg tactggagtt actggtccgc acctttcgcg
    17101 atctgcgtgc caacggcgaa aagaaaacgt caatggatac actgacagcg attatgtcca
    17161 ccgctgaagc cgtgaacgtg gcccacgcgg tgggcgtccg cgcctggttt ttagcgaatc
    17221 gagcaggtga gccagccgat ctggtggact gtattgccgg aaccatcgtc aaagataacg
    17281 aagaagaccg cgcgcgcctg cgccgttact tcgaacaacg tgttgccaca cataaagaag
    17341 ctcactggca ggcttattat caagctcgcc accgcctgcc gtgaggaaag atgcatgagc
    17401 gagccgttaa ttgtcggcat ccggcatcat agcccggcct gcgcccggct ggtgaaatcg
    17461 ttaatcgaaa gccagcggcc acgatacgta ttgattgaag gcccggctga ttttaatgac
    17521 cgggtagacg aactgttttt agcccaccag cttccggtag ctatttacag ttattgccag
    17581 tatcaggacg gtgcggcccc cgggcgtggt gcctggacgc catttgctga attttcgccg
    17641 gagtggcagg cgctacaagc cgcacgtcgt attcaggcac aaacttactt catcgatttg
    17701 ccttgctggg cgcagagtga agaagaggac gattcgcctg atacacaaga tgaaagccag
    17761 accttactgc tgcgtgccac ccgcatggat aacagcgata ccctgtggga tcacttattc
    17821 gaagatgaaa gccagcaaac tgcattaccc tctacgctgg cgcactattt tgcccaactg
    17881 cggggcgatt cccccggaga tgctctcaat cgtcagcgcg aagcctttat ggctcgctgg
    17941 attacatggg cgatgcagca aaacaatggc gacgtgttag tcgtctgcgg cggctggcac
    18001 gctcctgcac tggcaaaaat gtggcgcaaa tgcccgcagg aaatgaacaa gccagaattg
    18061 tcctcgctgg cagatggcgt tacaggttgt tatctcacac cctacagtga aaagcgcctt
    18121 gatgtgctgg caggatacct ttccggaatg cctgccccgg tctggcaaaa ctggtgctgg
    18181 cagtgtggct tacagcaggc aggtgaacaa ctgctgaaaa cggttcttac ccgtttgcgc
    18241 cagcacaaat tgcccgcttc taccgctgat atggctgctg ctcatctgca tgcgatggca
    18301 ctggcacagt tgcgcggcca tacactacct ctacgcactg actggctgga tgccatagct
    18361 ggctcgctga ttaaagaagc cctgaatgcg ccgttgccgt ggagctatcg cggagttatt
    18421 catcccgata ccgatccgat tttgctaacg gtgatagaca cattagcggg tgacggattc
    18481 ggtaaacttt ccccttctac gccacaaccg cctctgccaa aagatgtcac ctgcgaactg
    18541 gaacgtaccg gaatctctct tccggcggag cttaccttaa atcgctttac ccccgatgga
    18601 ctggcgcaaa gtcaggtgtt acatcggttg gcaatactgg agatcccagg ggttgtgcgc
    18661 cagcagggaa gtacgctgac acttgcaggc aacggtgaag aatgctggaa attaacccgc
    18721 ccgcttagcc agcatgcggc attgattgag gccgcctgct ttggtgccac actccaggaa
    18781 gccgcacgcc ataaattaga agccgatatg ctggacgcgg gtggaatcgg ctgtatcacc
    18841 acatgtctta gccaggcagc gttagcgggt ctggcgtctt tcagtcagca attactggaa
    18901 caactcacat tattaattgc ccaggaaaat caattcgccg aaatgggcca ggcgctggaa
    18961 gtgctatatg ccttatggcg gctggatgaa attagcggta tgcaaggcgc gcagatatta
    19021 caaacgacat tatgcgcggc catcgatcgc acgctgtggc tatgtgaatc caacggcaga
    19081 ccggatgaaa aggagtttca cgctcacctg catagctggc aggcgctttg ccatatcctg
    19141 cgcgatctac atagcggcgt taatttaccc ggcgtttcgc tttctgcggc ggtagcctta
    19201 ctggagcgcc gcagtcaggc gattcatgcc ccggcgctgg atcgcggcgc gactcttggc
    19261 gcactgatgc gtctggaaca tcccaacgcc agtgccgaag cggcgctgac gatgctggcg
    19321 cagttatccc cggcacaatc cggcgaggcg ctgcacggtt tgctggcatt agcccgtcat
    19381 caactggcct gtcagccggt atttatcgcc ggtttcagca gtcatttaaa tcaactgagt
    19441 gatgccgatt ttatcaatgc cctgcccgat ttacgcgcgg cgatggcctg gctaccacca
    19501 cgagaacgcg ggacgctggc gcatcaggtg cttgagcatt accagctcac acagcttccc
    19561 gtttcagcgc tgcaaatgct gttgcattgt ccaccacaag ccattgcaca tcatcaacaa
    19621 ctcgaacagc aggcactggc atcgctgcaa cactggggag ttttccatgt ctgaactgaa
    19681 cgatcttctg accacccgtg agctacaacg ctggcgatta attcttggcg aagcggcaga
    19741 aacgaccctt tgtgggctgg atgacaacgc ccggcagata gaccacgcgc tggagtggct
    19801 gtatgggcgc gatcctgaac ggctccagcg tggtgaacgt tacggtggat taggtcgttc
    19861 aaatctcacc acccctgagt ggattaacag cattcacacg ctgtttccgc aacaggtgat
    19921 tgagcggctg gaaagcgatg ccgtgctgcg ctacggcatt gaagatgtgg tgacgaatct
    19981 cgacgtgctg gaacgtatgc agccttctga aagcctgcta cgcgccgttt tgcacaccaa
    20041 acatctgatg aatcccgaag tactggctgc cgcccgccgg atagtgcacc aggttgtgga
    20101 agaaattatg gctcgactgg caaaggaagt tcgtcaggct ttttctggag tacgcgatcg
    20161 ccgtcgccgc tcatttattt cactggcgcg aaactttgat ttcaaaagta ctctgcgcgc
    20221 caacctgcaa cactggcacc cgcaacacgg caagttgtat atcgaatccc cccgctttaa
    20281 cagccgcata aaacgctata gcgaacaatg gcaactggtc ctactggttg atcaaagcgg
    20341 atcgatggtt gattcggtga tccactccgc agtgatggcg gcctgtttat ggcagttacc
    20401 cggcattcgt acccatctgg tcgcttttga caccagcgtg gttgatctca cggcagacgt
    20461 tgccgatccg gtagagctat taatgaaagt gcaattaggc ggcgggacca atatcgccag
    20521 tgctgtggaa tatggtcggc aacttattga acaaccagcg aaaagcgtca ttatcctcgt
    20581 gagcgatttt tacgaagggg ggtcatcatc attgctgacg catcaggtga aaaagtgtgt
    20641 ccagagcggc atcaaagtgc tgggactggc ggcgctcgat agcagcgcaa caccttgcta
    20701 tgaccacgat acggcccagg cgctggttaa tgtcggcgca caaatagccg caatgacacc
    20761 gggcgagctg gcttcctggc ttgcggagaa tcttcagtca tgaattcact acgtccggaa
    20821 ttattagaac tgacgccgca ggctctgacg gcgttaagca atgccggttt tgttaagcgc
    20881 agccttaagg aactggaaaa tggcaacgtc ccggaaatca gtcatgagaa cggcgcttta
    20941 atcgccacct tcagtgacgg tgtccgtacc cagctggcga acggccaggc actgaaagag
    21001 gctcagtgca cctgcggggc cagcggcatg tgccgtcatc gcgtgatgct ggtgttaagt
    21061 tatcaacgac tttgtgccac cgcccagcct acggaaaaaa aagaagaaga gtgggatcca
    21121 gcaatctggc tgaaagaact ggctaacctg cccgatgcca cccgcaaacg cgcgcaggcg
    21181 ctggtcgcta aaggcatcac cattgagttg ttctgtgcgc ctggcgaaat tccctctgcc
    21241 cgattaccga tgagcgatgt gcgtttttat tcccgcagca gcattcgctt cgcacgctgt
    21301 gattgcattg aaggcacact ttgcgaacat gtcgtactgg ctgtgcaggc cttcgtcgag
    21361 gccaaaactc agcaagcgga atttactcat ttaatctggc agatgcgcag tgaacacgtg
    21421 acatcatctg atgatccgtt tgccagcgaa gaaggcaaaa cgtgtcgtca atatgttcag
    21481 caattaagcc aggcattatg gctgggcggg ataagtcaac cgccaatcca ttacgaggcc
    21541 gctttcagtc gcgcgcagca agcggcggaa cgctgcaact ggcgatgggt gagtgaatcg
    21601 ctacggcaat tgcgcgccag tgttgatgcc ttccacgccc gtgccagcca ctaccatgcc
    21661 ggagaatgct tacgtcagct tgcggcatta aacagtcgat taaattgcgt acaagagatg
    21721 gcgcgacgcg acagtatcgg tgaagttcct cccatgccgt ggcgcacggt cgttggcgct
    21781 ggcattgccg gagaagcaaa acttgatcat ctgcggctgg tgtctttagg tatgcgttgc
    21841 tggcaggata ttgagcagta tggtttacgc atctggttta ccgatcccga cacaggaagt
    21901 attttgcatc tttcgcgtag ttggcagaga agcgaacagg aaaactcacc ggcagcgacg
    21961 cgtcggctgt ttagttttca ggctggcgca ctggcaggtg ggcaaattgt ttcacaagct
    22021 gcaaaacgca gtgccgatgg cgagctgctg ttagcgaccc gtaaccgctt gagtagtgtt
    22081 gttccactgt cgccagacgc ctggcaaatg ttgagcgcgc cgttgcgcca gccgggcatt
    22141 gtggctttgc gggaatattt acgccagcgt ccccccgcct gcatacgacc actcaaccag
    22201 gtcgataact tatttattct gccggtagcg gagtgtattt cgctcggttg ggatagcagc
    22261 cgccagacac tggatgcgca ggtaatcagc ggcgaaggag aagataatgt gctgacgtta
    22321 tcccttccgg catctgcctg ttctcctttt gccgttgaac gcatggcggc gcttttgcaa
    22381 caaacagacg accccgtgag tctggtttct ggctttgtca gttttgtaga ggggcaattg
    22441 acactggaac cacgggtgat gatgacaaaa acccgcgcct gggcgctgga cgcagaaact
    22501 gcgcctgtgg caccgctacc ttctgccagc gttctgcctg tgccgtctac cgctcatcag
    22561 ttgctgatgc gctgccaggc gttacttatt caactgctcc ataacggctg gcgctatcag
    22621 gaacagagtg ctattggtga ggcagagttg ctggcgaatg acctcaccgc ggtgggtttt
    22681 tatcggctgg cacatgtgtt gggacaattt cgtaatacag aaagcgaggc acgggtagaa
    22741 gcaatgaata acggtgtttt gctttgcgaa caattattcc ccatgcttca gcaacaagga
    22801 tgaaatagtg ctttttatta agagttctac tcctgttccg gactgctcac gccacagtat
    22861 taggcatatc ctatatagcc cctggtgaga gtcaccagtt ccttgattaa ataaaatgga
    22921 gttttacatg aaggctttca ataagctgtt ttcccttgtt gttgcatctg ttctggtttt
    22981 ctctcttgct ggctgcggtg acaaagaaga atcaaagaaa ttcagcgcca atctgaacgg
    23041 cactgaaatt gccattacct atgtctacaa aggtgacaag gtgcttaagc aatcttctga
    23101 aaccaaaatt caatttgcct ccattggtgc aaccaccaaa gaagatgccg ccaggacact
    23161 tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt gaagaaaaat taacctatac
    23221 cgatacctac gcgcaggaaa acgtgactat tgatatggaa aaagtggatt ttaaagccct
    23281 gcagggtatt tcaggaatca atgtttctgc tgaagatgcc aaaaaaggta tcactatggc
    23341 gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg
    23401 ccatgtttcg catggccgcc atacccgtct tagcttttct tcacatgctg gcgcgctgcc
    23461 agtccacgca gaaaataacg taaaaattga tcgccgcatt cgcggaagtt tttatgatcc
    23521 ggtgcacgca tcatcgccgt aatttccggc atcgaaacgc ggaactgctg ttcggtgagg
    23581 attgccagaa tgtcatcggt tttaagcgaa aacgcgatgc gtaatttttt cagcacgatg
    23641 ttgttattaa tgcgacgttc cggctccagt gccggagcag actcatcctt gccgcgtttt
    23701 tcataaatca ggccattgag gaatgaagac aaaacaatgt ccggacaacg ctgaaaaccc
    23761 tcttcgtctt ctttacgtag ccagacggcg atctgttccg cggtggcttc gacattaccc
    23821 agcgccagaa tacgcaccag gtcattatta ttggctttca aaatgtagcg cacgctgcgc
    23881 agaatatcgt tacttagcat gaggccttca ggtgttgatg aggcaaaaag ccattttagc
    23941 agtcttttac aggccaatcg cctcttttaa gcttttcaga taacggcggc tgaccggcac
    24001 ggttaagcca ttacgcaaaa tcaactcggc ctggccgtta tcttccagac ggatctcctg
    24061 taaatgcgcg aggttaacca gatactgacg atggcagcgc agtaatggtg tacgactttc
    24121 cagggtacgt aatgtcagtt cggtaaagcc ctctttccct tcgtgactgg taacgtagac
    24181 accgctcatc cgactgctga caaatgccac atctttcatt tgcagcaaat aaatccgact
    24241 atgccccgta caagggataa atttcagcgc ctgttgattt tccggtaaca gcgaaacatc
    24301 ctgcttgctg cgctcctgac gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat
    24361 tggcttcagc agataatcaa aggcatgttc ttcaaaggct ttaatggcgt attcgtcaaa
    24421 cgcggtgaga aaaacaatat acgggcgatg ttccgggtca agcatcccca ccatttccag
    24481 accactgatg cgcggcatct ggatatcgag aaacagcaca tccgggcgca gtttatgcac
    24541 cgcgccgatc ccttccacgg cgtttgaaca ctctccaacg atttcaatat cgctctgctc
    24601 ctgcaaaaat acgcgcaggt tctcccgtgc aagcggttca tcatcgacaa ttaagacttt
    24661 aatcatgcct cgtccctcca tggtagtcgt aacgttattc gggtgtaact atcaggctca
    24721 caggcgacgc ttatcccata gtcatcgcca aaccgttcac gtaaacgctt atccaccaga
    24781 ttcatcccca gcccactggc attggttacc ggttgataca aaccggcatt gtcttcgatc
    24841 tccagcatca aatgttgccc ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc
    24901 agttgtgatg tcccatgttt aatggcgttt tccactatcg gttgcagggt aaacgcgggc
    24961 aattgctgct gggataattc ttgcggaata gcaatgttga cctgcaaccg cgactggaag
    25021 cgcgcctttt caatttgcag ataagcgttc acatgttcaa tttcgtcggc gagagtaaca
    25081 aactccgaag gccgctttaa gtttttgcgg aaaaaagtgg aaagatactg caccagctgg
    25141 ctggcctgtt cgctgtcgcg gcggatcacc gctttaatgg tgttaagcgc attaaacaaa
    25201 aaatggggat tcacctgggc gtgaagcagt ttgatctctg actgggtgag catcgctttt
    25261 tgccgctcat attgcccggc gaggatctgc gccgaaagca gttgcgcaat cccctcgccc
    25321 agcgtgcggt tgattgaact gaataaacgg tttttggctt catacaattt gatggtaccc
    25381 atcactcgct gattttcacc acgcaacgga atgaccagcg tcgaccccag tttgcattgc
    25441 ggatgcaaag agcaacggta aggcacttcg ttgccatcag cgtagaccac ttcaccggtt
    25501 tcaatcgctt ttaaggtata agtcgaagaa atcggtttgc cgggtaaatg gtggtcgtca
    25561 ccaattccgg taaaggccag caatttctct cgatcggtaa tcgcgactgc accaatatcc
    25621 agctcctgat acagcacctg tgccactttc atgctgttca cttcgttaaa cccctggcgc
    25681 aaaatgcctt ccgtcgaggc cgccactttc agcgcagtgg cagaaaaagc cgaagtgtat
    25741 ttttcaaaca tcgcgcgttt atcgagcaat atacgcataa acaacgccgc gccgacggta
    25801 ttggtgacca tcattggcgc agcaatatta ctcaccagac gcaccgcatc ttcataaggt
    25861 cgggcgatcg ccaggatgat caacatttgc accatttcag cgacgaacgt gacggcaccg
    25921 gcggtaatgg ggttaaagac tttatcagtg cgcccgcgac ggatcaggat gctgtgaacc
    25981 aggccgccga gcaatccttc aacgatggtc gagatcatgc aacttagcgc ggtcatgccc
    26041 cccatcgaat atcgatgtaa cccgccggtc agaccaacca gcccaccgac gaccggaccg
    26101 ccgagtaagc cgcccattac cgcgcctatc gcacgggtat tggcaataga atcgtcaatg
    26161 tgcaacccaa accaggtgcc catgatgcag aagatggaaa agacgatgta gcagagaaat
    26221 ttatgcggca gacgaaccgt gacctgcatt aacggtatga ataatggcgt tttactcatt
    26281 aaccacgcaa tgactaaaaa aacgcacatc tgctgaagca gcagcaacac cagattaaaa
    26341 tcgtacatac ccgcaaacca cacttccctt taaaatgcgt aacatacatt gcttgcgttt
    26401 aactttcttt gaactcttgc agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc
    26461 ctggcaaaaa taaaatcacc ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc
    26521 aaaactgcgt ctaaagttaa accgggacct cgcgagcaag ggtgagacga tggcgcttta
    26581 cacaattggt gaagtggcgt tgctttgtga tattaaccct gtcacgttac gcgcgtggca
    26641 gaggcgttac ggattgctga aaccgcaacg gacagacggc ggccatcggc tgttcaacga
    26701 tgccgatatt gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag
    26761 caaagtaaaa atgctgctca gtaatgaaaa tgttgatgtg cagaacggct ggcgcgatca
    26821 gcaagaaaca ttactgactt acctgcaaag cggcaatctg catagcctgc gaacgtggat
    26881 caaagagcgc ggtcaggatt accccgccca gacactcacc acacatctgt ttattcctct
    26941 gcgccgacgg cttcagtgcc aacaaccgac tctccagacg ctgctggcga tcctcgacgg
    27001 cgtactgatc aactacatct ctatttgcct ggcttcggcg cgcaaaaaac agggtaaaga
    27061 tgcactggtg gttggctgga atattcagga taccactcgt ctgtggctgg agggctggat
    27121 tgccagtcaa caaggatggc gcattgatgt cctcgcccac tcgctcaatc aactacgccc
    27181 tgaactgttc gaaggccgta cattgctggt gtggtgcggt gataatcgaa cctccgccca
    27241 acagcagcaa ctcaccagtt ggcaagaaca aggccatgat attttcccac tcggcattta
    27301 atgattcgtt aacaaatgcg ctttactgta caatcctttc gttaacataa ggagtgcatt
    27361 atgcgcatag ctaaaattgg ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt
    27421 ggtggcgtca tgctcgcagg ttataccttt attttgcgtg ctggctgaac gcctgcacca
    27481 gcctttcaaa caggcggtct gcgatgatcg ccgccagtgc caccagtaac gctccctgga
    27541 tcacatacgc ggtattaaat ccgctaagtc cgatgatgat gggcgtaccc agcgtgctgg
    27601 cccctaccgt tgaggcgatc gtcgccgtac caatgttgat aatcaccgaa gttcgcacgc
    27661 cagccagaat caccggagcc gccagcggta gctcgacctt acgcagtcgc tgaccacgac
    27721 tcattcccat acctttcgca acttctgtca cgctggcatc aatcgctccc agcccggcaa
    27781 gtgtcgcctg caggacgggc agcacgccgt aaaggatcaa ggcgataatc gctggtttca
    27841 gaccaaagcc gatcaccgga acggcaatcg ccagcaccgc gacgggcgga aaagtctgcc
    27901 caacggcggc aatagtttcc accagtgggc gaaattccgc accccacggg cgagtgacag
    27961 caattccggc accagtacca atgatcaccg caaacaaact cgaaattccc accagccaga
    28021 aatgagccag tgccagagct gcaaaacttt cttgctgata aacgggtcgt ggcagttgtg
    28081 ggaacaaggc agcaaacagc ggctggctat aaggtagcca gaaaatcagt gccacaaaca
    28141 aagcaatgag ccagaacagc ggatcgcgca acatcttcat acgcttacgc ctcctccagc
    28201 agatcctgaa aatgcagcgt gccgcaaggc tggccctgcg tgttcaccac cggcagcacc
    28261 tcgcatcccc gcgcgacaaa cagagaaagc gcatcgcgta gcgtcatcct ttcttccagt
    28321 gcctcacctt cagcccgttc ttcacggcgc acgtaatctg ccacgctacg taacgaaagc
    28381 aggcgtacac ccagctcact acgaccaaaa aactggcgga caaaatcatt cgccggatga
    28441 gtcagcatcg ccagaggatt gccctgctgc acgacttcac cgtgatccat caataccaga
    28501 tgttctgcca gccgtagcgc ctcatcaata tcatgagtga ccagcacaat ggtacgcccc
    28561 tgcaaacggt gaatgcgcgt catctcttgt tgcaacgcgc cgcgcgttac cgggtccagc
    28621 gcgccaaaag gttcatccat cagtaagact tgcggatcgg cagccagtgc gcgcgccact
    28681 cccacacgtt gctgctgacc accggaaagc tgatgcggat aacgctcacg caaatttggc
    28741 tccagcccca gtagcgccat taattcgtcg atacgatcgt cgatccgcgc ccgtgaccat
    28801 ttttgcaatt gcggcacggt ggcgatgttt tgtgccacgc tccagtgggg aaacaggcca
    28861 atagattgaa tggcatagcc catccggcgg cgcaactcca gtactggcag cgagcgaatt
    28921 tcttctccgg caaagcggat caccccgctg tcatgctcca ccaggcggtt aatcattttc
    28981 agagtggtgg atttaccgga gcctgatgta ccaatcagca ccgaaaaact cccttcctga
    29041 aaattgagat tgagatcgtt aacggctttt tgtgcgccga acagtttgct gacatggcta
    29101 aattcaatca ttacgtttca ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt
    29161 aagcaccgcc agaacaatta ccgggatcac ccccagcaac actaaatcaa tggcactgct
    29221 tagcagcccc tggaaaacca gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac
    29281 cgccatgcca acggtttgca ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc
    29341 cagcggtaac tgaacatgca ggaaacgctg caccccgctc atccccatcg ctctggcgct
    29401 ctccagcaca tcgcgcggga tctggttcaa gccgactact acgccgcgca ccagcggcag
    29461 caaggcatag agcaccagcg caataagtgc gggtgtcatt ccggttcctg ctatgccgag
    29521 cgttcccagc cacggaaagg ccgtaaccag cgcggcaagc ggcgcaatca gcaggccaaa
    29581 gagcgctacc gaaggcacgg tctgaatgac attcagcaga gaaaaaatcg ccccctgccg
    29641 cgcggtggaa aagtagcacc agatgcccaa cggcacacca atcactaacg caggcagcac
    29701 cgcaccaaac agcaacgtca gatgttgtgc cagagcgtcg tcaaacacat cctgacggtt
    29761 ggcgtattct ttcattaacg agagatcgtt aagcgtgccg gagtacagca accacagcgg
    29821 aataatggca atctgcatat gcaacaacca gcgccacagc ggatgcgtgg agattcggcg
    29881 gatggcatcg ctacaggcca gcagtgccag cgccgcagcc agccagaaac cactgccgag
    29941 gctggtacgt gccagcgcac tgccattttg cgccagttgt gtcgccgcct ttccagctcc
    30001 ccacaccagc aatacgaaga cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt
    30061 agcgggaata aaacaggctg tcagccaggc gcaaccaacg cccaccagca tccagagagt
    30121 ttgcggccac agctgccaga gatgacgccc ctcacccgaa actaaacgat taggcgcgta
    30181 actgataaac ggcagcgccg ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg
    30241 attaatacgt agataagtca cgggaaatta cttcgtccac cctttttgtt tcaggtagtc
    30301 ggcagccact tttttggcat ccagtccttc cacagcaatg ctggcattca gttgctgcaa
    30361 tgttttttca tcgaggctgg caaagactgg ctgtagccac tgtgccattt gtggatactc
    30421 tttcagcacc gactcacgca ccactggtgc aggcgcgtag ataggttgca cgccttgcgg
    30481 atcgcttaag gtttgcagcc ccagcgccgc gaccgggccg tcagtgccgt aagccattgc
    30541 ggcattaacg ccagaggttt gctgggcagc ggctttgatc gttaccgccg tgtcgccacc
    30601 agccagtgac agcaactgat cctgaccgag cttaaagcca taggcttttt caaacgcggg
    30661 taaggcatcg gcgcgttcaa taaactctgc cgaggctgcc agtttgaagg tgccgccctc
    30721 ttgcagataa cgactcaggt cggcaagcga agtgagcttg tttttctctg ccacatcctg
    30781 acgcacggcg atggtccagg tgttatttgc aggtgcgggc gtcagccaga ttaacttgtt
    30841 ttgctctgca tcgagttttt tgactttctc gtaaccttgc tgcacgtttt tccacgctgc
    30901 atcgttttca tctttaaaga agaacgcccc attgccggta tattccggat agatatccag
    30961 ctcgccagaa gtgatcgcgc cgcgcaccac aggagtcgtt ccaagttgca ctttatttac
    31021 cgtaggaacg ccgtgacttt ccagcacctg caaaatgata ttgccgagca gcgcgccttc
    31081 cgtatcgatt tttgaaccaa ccttaacggg ggaagctgcc tgtagcggca ggctcacggc
    31141 tgccaacaaa accagtgaac ctgcccagac ctttgagagt ggcatgatgc tttcctcatt
    31201 cttttactgt tgttttcagc gaattaagag aaaagcatag ttgataatga cagggttagc
    31261 ctgtctgggt ggattcagtt gcagaatcag ataaatactt aaaaggcata ttttgcccgg
    31321 cgtttcttgc cggatgcggc gtaaacgcct tatccggcct acaaacatcg aaaattacaa
    31381 caactcaaac tcggctttct taacgcgtgc ggaatcagtg ccgataaaga cattgaactt
    31441 gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct caatatcaat
    31501 cgggaagctg acggtctgag tttcgcccgg tttcagagtg attttctcaa agcctttcag
    31561 ctgcttcacc gggcgactca tggaagccgt cacatcctgc aagtacatct gtactaccgt
    31621 ggccccttcg cgtttaccgg tgttcgtcac ctgcacgctg gccgtcactt tgccgtcacg
    31681 cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtgt agctcagccc
    31741 atagccgaac ggataaagcg ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt
    31801 cggcttgtcg gcattatacg gacgcccggt attcagatgg ctgtagtaca ccgggatctg
    31861 cccgacagaa cgcgggaagg acatcggcag cttgccggac gggttataat cgccaaacaa
    31921 tacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca gaatcgcatc
    31981 agcctgctga tcttctttca ccagcgccag cggacgccca ttcatcagca ccagcaccag
    32041 cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg gaatagtgat
    32101 atcggtacga ctggaagctt cgtgcgccat cccctgagct tcaccgacca cagccaccac
    32161 cacatcagat tgtttcgcgg tttgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg
    32221 atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt tgtcactggt
    32281 aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggcgt ttttaatccc
    32341 ggttagcaca gtaacagatt gatcggcgac acctgctgcc gaccagcttc ccatcacgtc
    32401 acgcttgctg tctgccagcg ggccaaccac cgcaatggtt gcagattttt tcagcggcag
    32461 cgtttcgaga cggtttttca gcaacaccag gctttcgcgc gccacttcac gcgcttcttt
    32521 gcggtgcagg cggctttcgg cattggtatc caccgggtca gactctttcg gccccaaatg
    32581 gctgtatgga tcgttaaaca atcccatatc atatttaacg ttcagtacat ggcgggcagc
    32641 atcgtccagc tcttccatcg tcactttgcc ggatttgatc aacccaggca gatacttcga
    32701 gtaatactcg tcgctcatgc tcatgttgat tccggacttc agcgccacgc gtaccgcatc
    32761 ctctgggtct gccgccgtgc catgtttaat cagttctttg attgcgccgt gatcggaaac
    32821 ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggagtcgga
    32881 ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcaccgc tgcctgcatc
    32941 cagccccgct ttgtacggcg gcatatagtc gttaaacagg cgctgcggac tcatatcgac
    33001 ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt
    33061 catcaccgag tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc
    33121 cattattgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg
    33181 atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc
    33241 ataagcagag acacgcccga ctgttttcac cgcatcgagg ttaaaagacg aggccagacc
    33301 gagactaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg
    33361 aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt
    33421 tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt
    33481 cgggttatcc gggccgacgc tgattaagcg cagctgacca attttctcat caactgtcat
    33541 tttcttaagc agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc
    33601 gaataaatca tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca
    33661 tagccatttc atatggattt ttcttccttt gttgcccgac gtggcagcga aaatggtgca
    33721 aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct
    33781 gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata
    33841 ttttttcgcc accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc
    33901 tttttgaatg aactttcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa
    33961 acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc
    34021 cctggctcac tactggaatt gtggcgggtg ctgaaagcct gcgtcaccgc tgacaaaatt
    34081 attctgatgc aggcggccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgat
    34141 tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgtactcggt
    34201 aagggcgaac aggtgctggc ctatccgggc accacgctct attcactgga aaaagccctc
    34261 aaaccgctgg gacgcgaacc gcactcagtg attggatcat cgtgtatagg cgcatcggtc
    34321 atcggcggta tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc
    34381 gaaatgtcgt tattcgcgcg tataaatgaa gatggcaaac tgacgctggt gaaccatctg
    34441 gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga cgatcgcatc
    34501 aaagatgacg atgtgcgcca cgatggtcgt cacgcccacg attatgacta tgtccaccgc
    34561 gttcgtgata ttgaagccga cacgcccgca cgttataacg ctgatccgga tcggttattt
    34621 gaatcttctg gttgcgcagg gaagctggcc gtctttgcag tacgtcttga taccttcgaa
    34681 gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa
    34741 atccgccgtc atattctggc taatttcgaa aatctgccgg ttgccgggga atatatgcac
    34801 cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag
    34861 ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg
    34921 gagaaagtga aattcttccg tccgcatttt accgaccgtg caatgcaaaa attcggtcac
    34981 ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat
    35041 ctgctgttaa aaatggcggg cgatggcgtc ggtgaagcca aatcgtggct ggtggattat
    35101 ttcaaacagg ccgagggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt
    35161 ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggctgtgca ttccgatgaa
    35221 gtcgaagaca ttctggcgct ggatatcgct ctgcggcgta acgacaccga atggtatgag
    35281 catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg
    35341 tgctatgtct tccatcagga ttacatcgtg aaaaaaggcg tggatgtgca tgcgttgaaa
    35401 gaacagatgc tggaactgct tcagcagcgt ggcgcgcaat accctgccga acataacgtc
    35461 ggtcatttgt ataaagcacc ggagacgttg cagaagtttt atcgcgagaa cgatccgacc
    35521 aacagtatga atccggggat cggtaaaacc agtaagcgga aaaactggca ggaagtggag
    35581 taataattac ggatggcaga gtatcgccat ccgtcattca tttaatcgtt ctgtgccgtc
    35641 tgccccgccg ccgccatttg ggcggctttt tgttttttat agctcaacgc tgctgccgga
    35701 acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg
    35761 gtatatttgc caaacgcgtc catcactacc agcgccaccg gtttattatt gataaccgtg
    35821 cgcatcacca gacaatggcc cgccgcattg gtaaaaccgg ttttggttaa ctgaatattc
    35881 cagttatcac gatacaccag atggttagta ttgcggaacg gcagcgtata cgtcgggtta
    35941 gagaaggttg ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atattgtttg
    36001 ctggcaatga gtagtttggt taagtcacgg gcggttgaaa cgttatgcac cgacaatccg
    36061 gttggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcatcgcc
    36121 ttaataaagg ctttgtaacc accgggataa tggtgcgcaa ggcttgccgc cgcgcggttt
    36181 tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct gttcagtcgt
    36241 acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt tagtttttca
    36301 tccagcggca gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct gatagacgca
    36361 atcggacgca ccagatccgg gtggttcgaa tagatcactt tgttggtatt cagatcaaca
    36421 atcatcgcgc taccggaggc aatttccggt tgtgacgcgg tggtagcggc tgccgttttc
    36481 gcaacggcct gcggtgcaaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact
    36541 cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacgggtt gcgcaccgcc
    36601 ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca
    36661 aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg cagcgcaaaa
    36721 gaactttcta gaacaaacga taaccgtagc cccatagtat aacggtaagg gcgagcagca
    36781 cttccagcac cagcacgcct atcgccagtg tcgaactgga gaagctaagt ccttcctctt
    36841 tgttgatatt caggaagctc ggaataccga gatacagcag gtagccggtg taaaacagcg
    36901 ccaccgtgcc gaccagcgca cacaaccaga ccagcgggta aagcgccacc agaccgctta
    36961 aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct
    37021 gcggataatt acgcgccatc caccagatga cccgccccat caccgcaacc ccagccagca
    37081 taacgccgta aaacaagaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag
    37141 tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa
    37201 tcgccgccat cagcaaaacg tggtgggtgt agtgatgaga aatcgtttcg ttttcgcgat
    37261 taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc
    37321 ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt
    37381 ttgttcactc atgtaatttg caccgtctga tgtacgtcct ttttcagcgt gaatgattca
    37441 cagggtgtat gcttacaggc aagcaaaggg agacagactg tcctatggat ctcaatacac
    37501 tcatctcaca atatggttat gccgcgctgg tgatcggtag cctggcggaa ggtgaaaccg
    37561 tgactttgct gggaggcgtt gcggcacatc aggggctatt aaagttcccg ctggtggtac
    37621 tttctgtggc gcttggcggc atgattggcg accaggtgct ttatctgtgc gggcggcggt
    37681 ttggcggcaa gctattacgc cgtttctcga aacatcagga taaaattgag cgggcgcaga
    37741 aactgatcca acgccatccg tatctgtttg tcattggtac gcgttttatg tatggctttc
    37801 gggtcattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccac
    37861 tgaatatcct cggcgcattt gcctgggcgt tgatttttac cactatcggt tacgctggtg
    37921 gtcaggtgat tgcgccgtgg ttgcataatc tcgaccagca tttgaagcac tgggtctggt
    37981 tgattctggt cgtggttctg gtggtaggcg tgcgctggtg gctgaaacga cgcgggaaga
    38041 aaaagccgga taatcaggcg taaaaccatt gcctaggata aggcgttcgc gccgcatccg
    38101 acatccggtg caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct acccaaatat
    38161 tacccattga aataaccacg cacctcactg cgctattctg ggttgaactg cggattcgcc
    38221 agcataaagc cgccatccac tatcaacgac tgcccagtgg tgtaatttgc gccctccgaa
    38281 caaagccaca ccaccaggct ggcaatctca tgcgttgcgc caaaacgccg caagggaatc
    38341 gaaggctccg cgccgggctt cacgtcgcca tcatccatgc cgttcattgg cgtggcgatc
    38401 gccccaggtg caaccgcatt caccaaaatc ttatgcctga ccagctccag cgccatcgct
    38461 ttggttaacc cgccgagcgc atgtttagcg gctgtgtagg cgctggcatc cggcaacggc
    38521 gtatgttcat gcaccgacgt aatgttgatg atgcgaccac cctgcccttg tttcaccatc
    38581 tggcgggcag caatttgcga gcataaaaat gcaccatcga catcaacggt aaaaatcttg
    38641 cgccactcat cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc cgcattattc
    38701 accagcacat caatacgccc cagccgttga atgagtttct ccagcgccag tgccccttct
    38761 ggtagattgc cgagatccag ctgcacgatc tccgcacgta cgccgtggct aactacctca
    38821 cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat atcaaacccc
    38881 tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc
    38941 gcgacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta tagaaggcgc
    39001 atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg aggcatccag
    39061 ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc
    39121 gctgacgcgg gaacccgcaa tgatgcccgc gttaaagcgc gcctgcgcaa gacctactac
    39181 acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt ttctccgcca gtgtctgaac
    39241 ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggc
    39301 gatagacaag ttgctttcgg cttttgcgat atcgaggttg gcgttaagac gaccactatc
    39361 gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag
    39421 atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa
    39481 tgccgctttt gccgcatcaa tagtgcttaa cgatgactca acgtaccagt gcgccgcctg
    39541 caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct gacttgccac
    39601 tttcggcaac gcgaccggat taagcttcaa tgcctttgtc tggtgatttg taagtgcgct
    39661 taaccgtgcc tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt
    39721 gctggcatta atatcggttt ccacaccttc aactgaagaa gtaatcccgt tctgatatag
    39781 ctggcgatcg gtggcgataa tggtgttctg ctctttttcg atttgctgta agacagtgtt
    39841 taacgccgcc tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg
    39901 gcgggtttgc tcgcgttccg ccgctcgtgc tttaaccgta cccaggcgag cagttacctc
    39961 tgcccgattc tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagtaccgtt
    40021 ggtgtaccac ggaccggtcg tacctgcggc aggatcgttc agagcaaacg gccccattaa
    40081 cccttctgca gacatttttt gccgctccat atccgcagaa aagtcgatct gcggaccatc
    40141 ctgagtagca actgccttcg cctgggcttc agccagttga atgcgctgtt cagccacctg
    40201 catatccggt gcgttctgta gtgcattgtt aattaaggaa gtgagttgat tatcgtgata
    40261 ctccagccac cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc
    40321 aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggtgcaca
    40381 cccggccagc atcagtaaca gcggaaaaca ggcgatggcg ggataaaagg aatcacgatt
    40441 catgggggaa taatcaggta agaaaaggtg cgcggagatt accgtgtgtt gcgatatatt
    40501 ttttagtttc gcgtggcaat acatcagtag caataaaacg acatatccag aaaaatatac
    40561 actaagtgaa tgatatcttc cgatttatct taatcgttta tggatagcgg caaagggctt
    40621 cgttttttcc tatacttatt cagcactcac aaataaagga acgccaatga aaattatact
    40681 ctgggctgta ttgattattt tcctgattgg gctactggtg gtgactggcg tatttaagat
    40741 gatattttaa aaatcagata tggcatataa gttgtcaggt caggaactag atattttgtg
    40801 tacttgattt gcaaacagct ccggtgttgg tggcgtcaaa atcgctgaag cgttccccga
    40861 aggccggggc agcccacatg gatgtgggct gagggcgcgt cttacaagga cgttacctcg
    40921 cgcccgaccc ggtagccgaa ggggataagc tgaaggtacc gcgcagcggc gattttgttc
    40981 gccagagccc gggggtgcag ggggaggagg cgattgtcct ccccctgcgc gctccttgcg
    41041 ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa caagatgatg aagctgaata
    41101 agcgattcat tgtctcagat aagctgcgat tccactatta tcgcagctta tcgatatcaa
    41161 tggcctgaat agcccttgca atatcagggg aattattcaa cacccgaaca tgctgaaata
    41221 attccgttgc ttcatcgtat tctttacgca aataactcaa ccactgttta atccgcgcaa
    41281 cgtgatataa cccggtatcg ccctgctttt ccagacgggt atatttttgc agcaaagcaa
    41341 ccacctccgg ccacggcatt cgcggttcgt tatatttcac cacacggctc aggttgggaa
    41401 tattgagcgc cccgcgacca atcatcactg cgtcgcagcc gctgatcgcc atgcattgtt
    41461 gcgcactctg ccagtcccag atttcaccgt tggcaatcac cggaatatta agccgctggc
    41521 gaatctcgcc aatcgcctgc cagtcaatat gttccgcgcg gtaaccctgc tcttttgtcc
    41581 gcccatgtac taccagctcc gtagcaccag cctgttgaac cgcatcggcg atttcaaatt
    41641 tcttctcacc gctgtcccag cccagacgca cttttacgct gacaggcaaa tgcgccggta
    41701 cagcttcgcg catcgctttt gccccctggt agatgagttc aggatctttc agtaacgttg
    41761 ccccaccacc gctaccgtta accgttttcg acgggcagcc gcagttaaga tccacgcccc
    41821 aggaacctaa ctccaccgca cgggcggcgt tctctgccag ccactgtggg aactgcccta
    41881 acaactgcac gcgaaccagc gtaccggatg gtgtccggct ggcattttgt agctcagggc
    41941 aaatgcgatg aaagactttt actggcagca gttgatccac cacgcggaca aactcggtga
    42001 tgcacagatc gtagtcgtta acttcggtca gtaattcccg caccagagag tcaagtacac
    42061 cctccatcgg agccagtaac acacgcatat catcacccgc aaaaaaatga ggcgctatgt
    42121 tagcgcctcc ggtcaacgga ttaaaggtct gcaaagaggg attgctgcaa actgcaatcc
    42181 ctctctatgc ttgatgtatg cctgagttcg ctgcgcattg cgtaataccg gggagcgaaa
    42241 aaataagcgc tgatttctta atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg
    42301 aatccatctc atctggggtg ttgattatga gtaagacact gaacattatc tggcaatatt
    42361 tacgtgcttt tgtcctgatt tatgcctgcc tgtatgcagg cattttcatt gcttccctgc
    42421 taccggtaac cattccgggc agcatcatcg ggatgctgat cctgtttgtc ctgctggcct
    42481 tgcaaattct tccggcgaaa tgggtcaatc cggggtgcta cgtactgatt cgctatatgg
    42541 cgctattgtt tgtgccgatt ggtgtaggcg tcatgcaata ttttgatttg ctccgcgcac
    42601 agtttggtcc ggtagtggtt tcctgtgcag tcagtacgct ggtggttttt ctggtggtga
    42661 gctggagttc acaactggtg catggtgaac gtaaagtcgt aggtcagaaa ggatcagaag
    42721 aatgatggcg aatatctggt ggtcattacc gctgacgttg attgtctttt ttgccgcccg
    42781 caaactggcg gcacggtata agtttcccct gcttaacccg ttgctggtag caatggtggt
    42841 gatcattcct tttttaatgc tgaccggcat ctcttacgac agctacttta aaggtagcga
    42901 agtgctaaac gatctgctgc aaccggcggt cgtcgcactg gcctatcctt tatatgagca
    42961 gctacaccag atccgcgcgc gctggaaatc gatcatcacc atctgtttta tcggcagcgt
    43021 ggttgcgatg gtgacgggga cttctgtggc attgttgatg ggcgcttcac cggaaatcgc
    43081 cgcgtcaatc ctgccgaaat cagtcaccac gcctattgca atggcagttg gcggcagtat
    43141 tggcggtatt ccggcaatca gcgcagtttg cgtgattttc gtcggcatcc tcggcgctgt
    43201 gtttggtcat acattgctta atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc
    43261 aatggggaca gcctcgcacg ccctcggtac ggcgcgctgc gccgagctgg attatcagga
    43321 aggtgcattt agttcgctgg cgctggtgtt atgcgggatt attacttcgc tgatcgcacc
    43381 gttccttttc ccgattattc tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt
    43441 ttgatgtaag tttcacgcgt tgcataatta atgagattca gatcacatat aaagccatag
    43501 cgggttcgta aactgttatc ccattacatg attatgaggc aacgccatgc atccacgttt
    43561 tcaaaccgct tttgcccaac ttgcggataa cttgcaatct gcactggaac ctattctggc
    43621 agacaagtac ttccccgctt tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac
    43681 ggggctggac gaagacgcgc tggcattcgc actacttccg ctggcggcgg cctgtgcgcg
    43741 tacgccattg tcgaatttta atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta
    43801 tttcggtgcc aatatggaat ttattggtgc gacaatgcag caaaccgttc atgccgaaca
    43861 aagcgcgatc agccacgcct ggctgagtgg tgaaaaagcg cttgcagcca tcaccgttaa
    43921 ctacacgcct tgtggtcact gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct
    43981 gcgtattcat cttccgggac gcgaggcaca cgcgctgcgt gactatctac cggatgcctt
    44041 tgggccgaaa gatctggaga ttaaaacgct gctgatggac gagcaggatc acggctatgc
    44101 gctgactggt gatgcgcttt ctcaggcggc gattgcggcg gcaaaccgtt cgcacatgcc
    44161 ttacagtaag tcgccaagcg gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg
    44221 cagctacgct gaaaacgccg cattcaaccc gactctgccg ccgttgcagg gggcgttaat
    44281 tctgttgaat ctcaagggtt atgattaccc ggatatccag cgcgcggttc tggcagaaaa
    44341 agccgatgcg ccgttgattc agtgggatgc cacctccgca acgctgaaag ctctcggctg
    44401 tcacaatatc gaccgcgtgc ttctcgctta agcctggtgc cggatgcggc gtgaacgcct
    44461 tgtccggctt gccagccctt tcctggtgtc gaaaatcccg gcaaacagtt tgccgtttct
    44521 tgcgcaaaac cagcgggtaa agtagcctga tggaaatttt ccttagatcg agtctcctgc
    44581 atgttaaagc gcgtgttcct cagcctgtta gtcctgatcg gcttgctgct gttgactgtg
    44641 ctcggcctcg atcgctggat gagctggaaa accgcgcctt atatctacga cgaattgcag
    44701 gatctcccct accgccaggt cggtgtggtg ctcggcacag caaaatatta tcggactggc
    44761 gtaattaatc agtattatcg ctaccgcatt caaggagcga ttaatgccta taacagcggt
    44821 aaggtaaatt atctattact gagcggcgat aacgcattgc aaagttataa tgagccgatg
    44881 accatgcgca aagatttaat cgcagccggt gtcgacccat cagatattgt tctcgattac
    44941 gcaggctttc gtacgctgga ttccatcgta cgtacacgca aagttttcga tactaatgat
    45001 ttcattatta tcacccaacg tttccactgt gagcgggcat tatttattgc gctgcatatg
    45061 gggattcagg ctcagtgtta tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt
    45121 cgcgaatttg ccgcccgttt cggtgcgctg gctgaccttt atatttttaa acgtgaaccg
    45181 cgttttttag ggccgctggt ccctattccg gcaatgcatc aagtgccgga agatgcgcag
    45241 gggtatcccg ctgtcacacc cgaacagtta cttgaattac aaaagaaaca aggaaagtag
    45301 ttatggatgt acagcagttt tttgtcgttg ccgttttttt ccttatcccg atattttgtt
    45361 tccgcgaagc atggaaaggc tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac
    45421 cggaaccggt gtatgtctgg cgagcgaaaa atcccggact cttcttcgct tatatggtgg
    45481 catatatcgg cttcggaatt ttatctatcg gcatgattgt ttatcttatt ttctatcgtt
    45541 aatattttct cttttctgtt aattattgag aataattatt acttcactca ataagctgcg
    45601 gatataacat tctgaaccgc agctatttgt gaatcctttc acagtttaaa tttccccacg
    45661 cgcatagcct taatatcagc acgcaatatt tatttagcgt tcgccttaat tactcatagc
    45721 attaaggaag atcacatgcc gcaacaaaat tatctggatg aactcactcc ggcttttacg
    45781 cctttactgg cgattaaaga agcctctcgc tgtttattat gtcatgacgc tccctgtagt
    45841 caggcttgcc cggcacagac cgatccgggg aaatttattc gctcaatcta ctttcgtaat
    45901 tttaaaggcg ctgccgagac aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga
    45961 gtgtgcccga cggagaaatt atgccaaagc ggttgtacac gtgccggtgt tgatgctccc
    46021 attgatatcg gtcgcttaca gcgttttgtt actgattttg aacaacaaac tgggatggaa
    46081 atttatcagc ccggtactaa aacgctcggc aaagtcgcca ttattggcgc aggtcctgcc
    46141 ggattgcagg ccagtgtgac gctgacaaac cagggttatg acgtcacgat ctatgagaaa
    46201 gaagcacaac ccggtggctg gttgcgtaac ggcattccgc aattccggtt accgcaatcg
    46261 gtgctggatg cagaaatcgc ccgtatagaa aaaatgggcg tgaccattaa gtgcaataac
    46321 gaaatcggta aaacactcac cctggagcag ctgaaagcgg aaaaccgcgc ggtactggtc
    46381 accgtagggt tatcgagcgg ttccgggtta tcgctgtttg agcatagcga cgttgagatt
    46441 gccgttgact tcctgcaacg tgcacgacag gcgcaaggcg atatcagcat tcaacaaagc
    46501 gcattaatta tcggcggcgg tgatgtcgcg atggacgtag ccagcacgct gaaagttctc
    46561 ggctgtcagg cggtaacctg cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa
    46621 aaagagttta ccagcgccag agagctgggc gtttcgatca tcgatggatt tacaccagta
    46681 gccgtggaag gcaataaagt cacctttaag catgttcgtt taccgggtga actaacgata
    46741 gcggcggaca aaattattct cgctgtaggt caacatgccg aacttgctgc tttttccgca
    46801 ctggaaccgc aacgtaacac catcgaaacg caacattacc agacccgcga cccgcaagtc
    46861 tttgctgctg gcgatattgt tgagggtgac aaaaccgtgg tctatgcagt gaaaaccggg
    46921 aaagaagccg ccggggcgat tcatcactat ttagagggag cttgctcatg ttaacgaaag
    46981 atctttcgat tactttttgc ggcgtgaagt ttcccaaccc gttctgcctt tcttcttcgc
    47041 cggtaggcaa ctgctatgag atgtgcgcca aagcctacga cacaggttgg ggcggcgtgg
    47101 tgtttaaaac gatcggtttt tttatcgcca acgaagtctc gccgcgtttt gatcatctgg
    47161 tgaaagaaga taccggtttt atcggcttca aaaatatgga gcagattgct gaacatccgt
    47221 tggaagagaa tctggccgcc ctgcgtcggc tgaaagaaga ttacccggac aaagtattga
    47281 tcgcttcaat catgggcgaa aatgagcagc aatgggaaga gctggcgcgt ctggtgcaag
    47341 aagctggcgc ggatatgatc gagtgtaact tctcctgtcc gcaaatgact tctcatgcga
    47401 tgggtagcga tgtcgggcaa agcccggagc tggtagaaaa atattgtcgg gcagtaaaac
    47461 gtggttccac gctgccaatg ctggcgaaga tgacgccgaa tattggtgat atgtgcgaag
    47521 tggcgctggc ggcgaagcgc ggcggcgcag atggcattgc ggcgattaac accgttaaat
    47581 ccatcaccaa tatcgatctt aatcagaaaa ttggtatgcc gatcgttaac ggtaaatcga
    47641 gtatttccgg atattccggt aaagcggtaa aaccgatcgc cctacgcttt attcagcaaa
    47701 tgcgcaccca tccagaactg cgcgatttcc cgatcagcgg tatcggcggc attgaaacct
    47761 gggaagatgc ggcggagttt ttattgctcg gttcagcgac gttgcaggtg accaccggca
    47821 ttatgcagta cgggtatcgg atagtggaag atatggcaag cgggttgtcg cattatctcg
    47881 ccgatcaagg gtttgattcg ttgcaggaga tggtaggtct ggcgaatcac aatattgtcc
    47941 cggcggaaga tttagaccgc agttatattg tctatcccca tatcaatctt gataaatgtg
    48001 ttggctgtgg acgctgttat atttcctgtt acgacggcgg tcaccaggcg atggaatgga
    48061 gcgagaaaac ccgcacaccg cattgtaata ccgagaaatg cgtgggttgt ctgctttgtg
    48121 gtcacgtctg cccggtaggt tgtattgatc ttggggaagt gaagtttaag aaaggcgaga
    48181 aagaacaccc ggtaacgttg taaaaccagc gacgcatccg gcatttagtt cctgtcgcca
    48241 gatgcggcgt gaatgcctta cccggcatac aaaacaatat aaatcagtat gttgtgctca
    48301 ttgtaggccg gataaaacgc gccagcgttg catccggcat ttggctcctg tcgccggatg
    48361 cggcgtaaac gccttatccg gcctacaaaa ccacacagat catgatgtta tgtgcgttta
    48421 tgaacgaatg cggaccacat tcacatcatt tcttacgcgc gtatttcagt gaatccagcg
    48481 ccacggcaaa gataataatt gcccctttga tgatgtactg ccagtacggg tttacgccga
    48541 tgtacgtcag gccatagttg atgacggtaa aaataatcac cccggtcacc acgccaatca
    48601 ccgtccctac accgccgctg aacgatacgc cgcctaccac gcacgctgcg atagcatcca
    48661 gctcatacat gaagccgagg ttgttggtgg cagagccgat acgtccggct tctaacatcc
    48721 cgccgaaggc atagaacaca ccagacaacg cgtagatcat cagcaggttc aggccgacgt
    48781 tgacaccaga tactttcgcc gcttccgggt taccgccaat ggcaaaaatg ttcttgccga
    48841 agcgggtttt gttccataac acccagacaa acaccaccgc aatcaacgca tagaaggtga
    48901 tgtaagagag acggaaactc cccagcgcaa taaagccttg ggcaaaagtg gagaaaccac
    48961 tgtcaaaacc ggaaattggc gacgccccga caaagtcgta atagagcgag ttaatgccgt
    49021 agacgataat catcgtaccg agcgtggtaa tgaacggcgt cacgttgaga taggcgataa
    49081 tcaagccgtt gatcaaaccg atcaccgcac caatggcaca gacaatcaga ataaccagcg
    49141 caatcggcat cgtcgccatt tccgggaaca ctttgttggc gttatccatg gactgcaata
    49201 acgtcgctgc tactactgcc gccagcccca cctggcgacc tgcggaaagg tcagtcccct
    49261 gggtgacaat taaccctgcc acaccaagcg cgataataat acgcaccgat gactgggtga
    49321 gaatattact taagttcaac agacttaaaa atgttgggtc ctggaaaata ataatcgcca
    49381 gcaaaactaa aagaacgacg taaataccgc cctctttcag gtaagtaaga aaacttttct
    49441 tatttaacgc actcatggga agcccctaat cttaaaggtg caaagacgca agacgcagaa
    49501 tttcgctttg cgttgttgtt tttgtatcga caattccgga aacgagacca ttgctcatga
    49561 ccagaatacg gtctgttatc cctaacaact ctggcatttc ggaggagata ataataatcc
    49621 ccttgccttt cttcgccagt tcggcaatta actgataaat ctcaaactta gccccaacat
    49681 caataccgcg cgtcggttca tcgagcatta atatttctgg ttgcgttaac agccagcgac
    49741 caataatcac tttttgctga ttaccaccgg agagcgaacc aatttgcgtc cgatgccccg
    49801 gtgtttttac ccgcatcgaa tcaatcaccc actgggtatc gcttttcatc cgcgagttat
    49861 ccagtaaacc aactttattt ttatagttgc ggatattgga aattaacgag ttaaagccaa
    49921 tatccagata ggcataaatt cccgttgagc ggcgctcttc agttaccagt gcaaatccgt
    49981 ggtttatggc ttcgttggca ttatggttat tgatcttttt gccgtgcaac gtaatggtgc
    50041 cagccgattt ctcgcgaata ccaaataacg tctcaacaat atcggtacgt ttcgctccca
    50101 ccagaccggc aataccgagg atctcccctt tatgcagatc aaacgagaca tcgcgaatcg
    50161 acggctggcg cagcgacgtc aggttacgca cttccagaat cacttcgccc ggcttgtttt
    50221 ctttgtcagg gaaacgctgg ttaagagaac gcccaaccat catggcgatg attttgtcca
    50281 tcgtcagtcc tgccagcggt tcggtagcga tccactgacc gtcgcgcaat acggtaactt
    50341 catcacataa ctggaagatt tcttccattt tatgcgagat ataaacaata ccgcagccgc
    50401 gctcttttaa tttacgaata atagtgaaca gatgattgac ctctttttcg gttaacgaag
    50461 aagtcggttc atccataatc acaattttcg cgttatagga aaacgctttg gcgatttcga
    50521 tcatctgcat ttgcgaaaca gataatgtac cgacgcgcgc acgcggatcg atatcaatat
    50581 ccagttcatc aaaaatcgct ttggtttcgc ggtacatttt gtcctgatcg acaaacatgc
    50641 ctttggtggg atatcgcccc agccacatgt tgtccatcac cgaacgttgt aataccaggt
    50701 ttaactcctg atgtaccatc gaaataccat tttccagtgc ttctttggcg gaatggaaat
    50761 cgatctcttt accctggaat aaaatggtgc cggagtcttt ttgataaata ccaaacaggc
    50821 attttaataa tgtcgatttt cctgcaccgt tttcccccat taatgcatgg atagaatgtg
    50881 gccggacttt taagttaacg ttatcaagtg ccttaacacc aggaaaagac ttgttgatac
    50941 cgctcatttc caacaagtat tcacctgatg acggagtcgt tgagctgacc atataatttt
    51001 accttgttgg tcatacaata agggcgcagt aaacgactgc gcccaatcag tcttatttct
    51061 tgctgaattc agccaggttg tctttatcta cgccaacata aggtacgcgg accactttgt
    51121 tgtcgatttt ccagttggtg ccatcagccg cacctttgcc atcggccagg tttttcgcca
    51181 gatcaaaggt cgctttcgcc tggttgttag catcgttcag tacggtgccc gccagtgcac
    51241 cggatttcac cagcgccagc gcttctggca gcgcatcgac gccaaacacc ggaatgctgg
    51301 acttgttgtg tgctttcagt gcttctaccg cgcccattgc catcgcgtcg ttgttggcga
    51361 taaccacctc gattttgttg gcgttcgggc cagacagcca ggcgtccatc ttatctttcg
    51421 cctgagcggt atcccacata gcggtatcta actgtaactg ttcagtttta atgcctttgt
    51481 cgttcaattc tttaatcacg taagtggtac gtgcttctgc atccggatgc cccggctcac
    51541 ctttcagcag tacgaattga atctgaccgt ctttgttcag atcccaaccc tgattcgccg
    51601 cccagtgttt agcaatcaaa tcgccctgaa taatgccgga ctctttggag tcagtgccaa
    51661 cgtagtaggc tttgtcgtag ctatccagcg ccttacgaga cggttctttg ttgaagaaaa
    51721 ccaccggcac gttttgccca cgcgctttct caatcaccgt acccgcagct gccgggtcaa
    51781 ccaggttgat tgccagtgcc ttcacccctt tcgccagcaa tacgtcgatc tgatcgttct
    51841 gcttggactg gtcattctga gaatcattca tcagcagctg aacatctggc gcggctttcg
    51901 catcttgctc aatagccttg cgcactacag acataaagtt atcgtcgtac ttatagattg
    51961 ttacaccaat gcgagtatca gcagcgtgtg cagcggcacc gaataacatg ctggccatca
    52021 cagcagacag ggttaacacc ttcttattca tggtatctcc ggtttttctt atgcagggta
    52081 gtgcttgaga taaatgctcg gcggggcagt agagttaatg aagtgttact gaacgccgaa
    52141 gctcactttt taaaattcgt tcttccatgc ccggtaacgc tccagaaaac ggctttaatt
    52201 gttgttatga cgctattacc tcggtgaaag tgattattca ctgttacata tgggttacaa
    52261 cgttaaaacg gtgcaatcat agccatcaca ttgttaagat actgtgaaat cactcacaga
    52321 ttgaaagcgg ttacatcgcc tgatttgttg agttagtgat cgccgccgca ttctggcgtg
    52381 ttgcgacaga atggcgacgc actaacgtcg gcataaaaca gtggctggca cgaggatcaa
    52441 tattgtctgc tgccccctgc aaggccagtt cggtagctaa tttagccatt gaagcaatgg
    52501 gataacgcac ggtcgttaat tgcgggtcgg tgtaacgggc gatgggaata tcatcgaaac
    52561 cgatgattga gagatgtagc ggaatcgcaa tgccattatc ttttaatgct gtcagtgcgc
    52621 cagcagccat attgtcgtta taagcaaata cagcggttag ttgtagattg cgccccagca
    52681 gttcaaccat cgccgcctca ccgcccggca tgtccggcgt accagtgcca atccagcttt
    52741 ccggcggaat aatatcctgc tctttcaacg cactcatcca gcctgcttta cgcatggcgt
    52801 catcttcaat gccgtggctg gaagaaagat aaccaatacg ttgatgaccg ttattcagca
    52861 acatgcgcgt cgccattcgg gcaccgctga gattatccag gcaaacgcaa cgatgggcgt
    52921 accccggcac aacgcggttg attaacacca taccaggaat gttatccata aattgtgcca
    52981 gttcatcgtc actcaatgct tttgagtgaa caatcaacgc attacaacgc tggcgaatta
    53041 acacctcaat ggcgtgacgc tctttttccg cttcatgata gctattgccg attagcacgt
    53101 atttctgatg ctgctgggcg accagatcca ccgcttttac cagcgcgccg aaaaaagcat
    53161 cagaaacgtc catcaccacc acgccaatgg tgtcgctaac ctgagttgcc agcgcctggg
    53221 cattggcgtt tggccgataa tccagctcac tcacagcttt cattactgct tcacgcgtgt
    53281 cggcactgac cagcgtgctg ttattgagca cccgggaaac cgttgccaca gagacgccag
    53341 cctgacgcgc tacatcacga atggtgatca tattcactac ctgttatgag attgcagtaa
    53401 atgactgctt gctggcggct attttgtcag cactgaatac aggacttcgt gaatcgagtc
    53461 acagcaatgg aaacggttac agccgttttg ttaacgaatg tgatccagat cgttatcttg
    53521 atgttttaga tattgtcggt ccggcagcac gcaaagttaa ctgacgccag agccattcca
    53581 ccggcccctg gcggaagtaa cgcagccaga taacagagaa gaagatattc gccagccata
    53641 ccggaataac aaacgccagc agctccagac ggtcaaactg cataaacaaa ctgaggtggt
    53701 aaaaaagcgt agtacaaatc agcgtttgca atagatagtt ggtcagtgcc atccgtccga
    53761 cacaggcgat tgccagcacc agcttaaagc ggcttaattg cggccagaag ccataaaaca
    53821 gcgacgcata gccaatcgcc tgaaacggcg cactcagttc ccgcggcatt tgaagtaaga
    53881 aggcacacca gcgatacgcc cagtccagct gccattgcag ggcgatggca ggaaggttaa
    53941 tgatcacccc aatcgccacc agcacaaaac cagtacgacg atagtgacgt aagctgaact
    54001 gccctttcag ccagccgctg cgcatcagcg ccgcaccaat gagcatcatc cctgccagtt
    54061 gccagccata ctgtgcgccc agtgccagta aactgttgcc aacaccatcg gcacgattat
    54121 tgatcgcttc aacaccaccg tgaagcttcc agtatttttc atacaaaata gccgatgcat
    54181 ccggcgtcca ggcgcggctg gtctggctgt cggaaatcag ccccaataac agcaaaacgc
    54241 caagccccac cagataaagc atgacgcctg tattaaacag gctttttacc gatggcgcat
    54301 cgcgcaccag ccgccagcag attaagccca ccagtccgta agccagcaga atatcgccgt
    54361 cccagaacaa taaaccgtga ataaagccca gcaagactaa cagcgttaac cgcgactgga
    54421 tccagcgtct gccacggggc agcaacattt gcaggcccgc accaaacagc agcgcaaaaa
    54481 gcgtgaggaa tttcacctgg ccgatgagat cgagaaatgc ccaggtccat gcatcctgcg
    54541 gcgtaatagc gccgtaccag gcgggattga gataagccgc ctttggtagc ccaaaggcgc
    54601 tgatgtttaa tagcaggatc cccagaatgg cgacgccgcg aacaaaatcg agcgtgacgt
    54661 tgcgctccat ggctcctgcc ttttaatcag ttgtgatgac gcacagcgcg cagaaactcg
    54721 tggcgcgtat tctgactgga tttgaacaat ccaccaagag aggtcgttgt tgtggcactg
    54781 gttgcgtcgc ggatgccacg cgctttcacg cagtaatgca ccgcgtcgat agagacagcc
    54841 acgttattgg tgcccagcag cgtttgtagc gcaatgagaa tttgctgcgt cagacgttcc
    54901 tgcacctgcg gacgctgggc aaagaactgc acgatgcggt taatttttga cagaccgatc
    54961 accgaatctt tcgggatata agccaccgtc gctttgccat ctatggtaac aaaatggtgt
    55021 tcacaggtgc tggtcagggt gatatcgcgc acggtgacca tttcatcgac cttcattttg
    55081 ttttcaatga gggtgatttt cgggaaattg gcgtaatcca gaccggagaa aatttcatcg
    55141 acatacattt tagcgatgcg atgcggcgtt tccatcaaac tgtcatcagc caggtcgaga
    55201 ttcagcagct gcatgatttc ggtcatatga ccagcaataa ggcttttgcg cgtttcgtta
    55261 tccatttcat gcacgggcgg gcgcagcggt gtttccagtc ctcgcgcaac taacgcttca
    55321 tgaaccaggg ccgcttcttt actgagtgat ggcatttatg atttctcctg caggtgtgac
    55381 gcctccgccc tgcgtggggg caaagttatt aagctgattt acagcctgat tattgtgcgt
    55441 gaggcggcgc acataatcca gtattcacag cgataattat tgtaattgcc gctgcctttc
    55501 atcagcagat gttaaaacat cgttatgcaa atacggaagt gaaagttact cacagcacat
    55561 tgaataaacg gtatgatgaa gaaattgcaa acaacacaac aaggagccac gcatggaaat
    55621 gctcgaagag caccgctgtt ttgaaggctg gcagcaacgc tggcgacacg actccagtac
    55681 cttaaactgc ccgatgacgt tcagtatctt tctcccacca cctcgtgatc acaccccgcc
    55741 accagtgctg tactggcttt ccggattaac ctgcaatgac gagaacttca ccaccaaggc
    55801 gggtgcccag cgcgtggcgg cggaactggg gattgtactg gtaatgccag acaccagccc
    55861 gcgcggcgaa caggttgcca acgacgatgg ctacgattta ggccagggcg cggggtttta
    55921 tcttaacgcc acgcaaccac cgtgggcgac gcattaccag atgtatgatt atctgcgcga
    55981 tgaattaccg gcactgattc agtcgcaatt taatgtcagc gaccgctgcg ctattagcgg
    56041 tcattccatg ggtggtcacg gcgcgctgat tatggcgctg aaaaatccgg gtaaatacac
    56101 cagcgtttcc gcctttgcgc caattgtgaa tccgtgcagc gtcccgtggg gaatcaaagc
    56161 gtttagcact tatttaggtg aggacaaaaa tgcatggctg gaatgggaca gttgcgcgtt
    56221 gatgtatgcc agtaacgcgc aggatgcaat cccgacgctt attgatcagg gtgataatga
    56281 tcagtttctt gctgaccaac tgcaacctgc agtactggca gaagccgcgc gccagaaagc
    56341 gtggccgatg acgctgcgta ttcagccggg atatgatcac agttactact tcatcgcctc
    56401 ttttatagag gatcacctgc gcttccatgc gcagtattta ctgaagtgaa agtccgcccg
    56461 gttcgccggg catcttctca tcagaagcga taatccactg ccataaagta acgacgtacg
    56521 tcttcgttat agctgtagtc gtcacgactg agatccttgt cgccaaggtt cagcacgcct
    56581 gcacgcagtt tgacgtcttt agtcacctgc caggccgcgc cggtattcca gatggtataa
    56641 ccgcccggtg ttttagccgt cgcgctgtcg gcgcgtttct gcccggtata gtgaccagaa
    56701 acatagaatg accagtcttc cagcgccagc ggtttccagt ccagcgtacc gttagcagta
    56761 tggaacggca gatcggatag cggtttgttt tcgccgttgc tgacatcacg accatcgttg
    56821 taggtgtagt tgatcgacag tttccattca tcgttgaacg gaattttcag ttcggtttcc
    56881 acgccctgaa tacgtgcttt gttaacgttg tagtagctaa ataccggtat gcgccgtccg
    56941 ttagcgcccg tctcaaaacc gacaaagttt tggtagcccg gtgcagcgtt gacgtcagac
    57001 gtacggctaa tgctgatacg atctttcaca tcgttacgga aaacggtaac gctggattca
    57061 accccttcca gccagccttc ttcgcccatg tagtaaagcc ccagttccca actttcgctg
    57121 gtttctggtt tcagatccgg gctacccaca atcttacatg cgccacggca ggaattgctc
    57181 gtccagtcag ggctaagttg caacagagaa ggtgctttaa atgccgtcgc ccagccccct
    57241 ttcaccgtta cggtgtcggt ggcgttataa accaggtagg cacgcggact ccagtgttca
    57301 ccgtaggttt cgtgatcgtc catacgcacg ccggtcgtca gcgccagcgg ctcgaagatc
    57361 cgccattcat cttccacaaa cagcgcgtac tggctggcag acgttttgga gctggttccc
    57421 ccggtcaggt tcaccgcatc gctaagtttg tcgtgacgcc attcaccgcc aaccgtgaga
    57481 aactgattaa tcgccgtcag cggcaacgtg tatttgccgt cgaccgtatt gctttcggaa
    57541 gttatcgggc tgctgttgcc agggtttttg ttctcgactt tctcaccgta gtatttcagt
    57601 tcgctggtgc cgtaatccca acgcccatta tggctgacgg agtagttctg gcgttccagg
    57661 cggtttttgt ccagcgagtc ggaatcacga tcctgacggt cgaaaccgta tccggcagta
    57721 aaatcgtgat tttgattcgg tgtccaggca aattcgacat tgccgtcgcg gctggagaat
    57781 ccttcaatac gcggcgtttc tccggtatcg gtggtcgttg agttttgcgg gtcatccttt
    57841 tcacgttttg ccaggctgcc gtaagctttc attcccagca cgccgtcaat taatggtccg
    57901 ctggtaaaga actgaccgtt ataggtatca ccgcgatcgc gatgttcctg aacggtggta
    57961 tcgacggtaa cggtgcccga ccatttctga ccgatttttt tggtgatgat attcactaca
    58021 ccgccgagcg catcggaacc gtacagcgac gacatcgggc cacggaccac ttcaatacgt
    58081 tcgatggaat cgaccgggat ccagttcaga tcgaaatcat tgtggcggaa gacggcattg
    58141 cgggagttaa cgcgtttacc gtcgacaaga atcagggtgt agctgctgtc cagaccacga
    58201 atacttacgc ccttacggtt atccccttcg ttcgtcagtt gtacgc
//
LOCUS       DAJOTB010000013        53254 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJOTB010000013 DAJOTB010000000
VERSION     DAJOTB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.013"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..184)
                     /gene="cspA"
                     /locus_tag="OHN28_000802"
     CDS             complement(<1..184)
                     /gene="cspA"
                     /locus_tag="OHN28_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCQ2296243.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKG"
     gene            complement(465..755)
                     /locus_tag="OHN28_000803"
     CDS             complement(465..755)
                     /locus_tag="OHN28_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCQ2296244.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1189..1899
                     /locus_tag="OHN28_000804"
     CDS             1189..1899
                     /locus_tag="OHN28_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCQ2296245.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1949..2923)
                     /gene="ghrB"
                     /locus_tag="OHN28_000805"
     CDS             complement(1949..2923)
                     /gene="ghrB"
                     /locus_tag="OHN28_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCQ2296246.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVAD"
     gene            complement(3027..3686)
                     /locus_tag="OHN28_000806"
     CDS             complement(3027..3686)
                     /locus_tag="OHN28_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCQ2296247.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3839..6172
                     /gene="bisC"
                     /locus_tag="OHN28_000807"
     CDS             3839..6172
                     /gene="bisC"
                     /locus_tag="OHN28_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCQ2296248.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(6141..6581)
                     /locus_tag="OHN28_000808"
     CDS             complement(6141..6581)
                     /locus_tag="OHN28_000808"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2296249.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6578..7141)
                     /gene="tag"
                     /locus_tag="OHN28_000809"
     CDS             complement(6578..7141)
                     /gene="tag"
                     /locus_tag="OHN28_000809"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCQ2296250.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7299..7997
                     /locus_tag="OHN28_000810"
     CDS             7299..7997
                     /locus_tag="OHN28_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2296251.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8226..9434
                     /locus_tag="OHN28_000811"
     CDS             8226..9434
                     /locus_tag="OHN28_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296252.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9758..11449
                     /gene="eptB"
                     /locus_tag="OHN28_000812"
     CDS             9758..11449
                     /gene="eptB"
                     /locus_tag="OHN28_000812"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCQ2296253.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11541..11617
                     /locus_tag="OHN28_000813"
     tRNA            11541..11617
                     /locus_tag="OHN28_000813"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11575..11577,aa:Pro,seq:cgg)
     gene            12361..13968
                     /gene="dppA"
                     /locus_tag="OHN28_000814"
     CDS             12361..13968
                     /gene="dppA"
                     /locus_tag="OHN28_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCQ2296254.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14275..15294
                     /gene="dppB"
                     /locus_tag="OHN28_000815"
     CDS             14275..15294
                     /gene="dppB"
                     /locus_tag="OHN28_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCQ2296255.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15304..16206
                     /gene="dppC"
                     /locus_tag="OHN28_000816"
     CDS             15304..16206
                     /gene="dppC"
                     /locus_tag="OHN28_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCQ2296256.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16217..17200
                     /gene="dppD"
                     /locus_tag="OHN28_000817"
     CDS             16217..17200
                     /gene="dppD"
                     /locus_tag="OHN28_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCQ2296257.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17197..18201
                     /gene="dppF"
                     /locus_tag="OHN28_000818"
     CDS             17197..18201
                     /gene="dppF"
                     /locus_tag="OHN28_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCQ2296258.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18231..19502)
                     /locus_tag="OHN28_000819"
     CDS             complement(18231..19502)
                     /locus_tag="OHN28_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2296259.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            19978..20085
                     /locus_tag="OHN28_000820"
     CDS             19978..20085
                     /locus_tag="OHN28_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2296260.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20461..20568
                     /locus_tag="OHN28_000821"
     CDS             20461..20568
                     /locus_tag="OHN28_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2296261.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVSWLNKRK"
     gene            20944..21051
                     /locus_tag="OHN28_000822"
     CDS             20944..21051
                     /locus_tag="OHN28_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2296262.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21137..22816)
                     /gene="bcsG"
                     /locus_tag="OHN28_000823"
     CDS             complement(21137..22816)
                     /gene="bcsG"
                     /locus_tag="OHN28_000823"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCQ2296263.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPHYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPIVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22813..23004)
                     /gene="bcsF"
                     /locus_tag="OHN28_000824"
     CDS             complement(22813..23004)
                     /gene="bcsF"
                     /locus_tag="OHN28_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCQ2296264.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23001..24572)
                     /gene="bcsE"
                     /locus_tag="OHN28_000825"
     CDS             complement(23001..24572)
                     /gene="bcsE"
                     /locus_tag="OHN28_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCQ2296265.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            24845..25033
                     /locus_tag="OHN28_000826"
     CDS             24845..25033
                     /locus_tag="OHN28_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCQ2296266.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25045..25797
                     /gene="bcsQ"
                     /locus_tag="OHN28_000827"
     CDS             25045..25797
                     /gene="bcsQ"
                     /locus_tag="OHN28_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCQ2296267.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            25794..28412
                     /gene="bcsA"
                     /locus_tag="OHN28_000828"
     CDS             25794..28412
                     /gene="bcsA"
                     /locus_tag="OHN28_000828"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCQ2296268.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            28423..30762
                     /gene="bcsB"
                     /locus_tag="OHN28_000829"
     CDS             28423..30762
                     /gene="bcsB"
                     /locus_tag="OHN28_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCQ2296269.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            30769..31875
                     /gene="bcsZ"
                     /locus_tag="OHN28_000830"
     CDS             30769..31875
                     /gene="bcsZ"
                     /locus_tag="OHN28_000830"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCQ2296270.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            31857..35330
                     /gene="bcsC"
                     /locus_tag="OHN28_000831"
     CDS             31857..35330
                     /gene="bcsC"
                     /locus_tag="OHN28_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCQ2296271.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            35412..37400
                     /gene="hmsP"
                     /locus_tag="OHN28_000832"
     CDS             35412..37400
                     /gene="hmsP"
                     /locus_tag="OHN28_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCQ2296272.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37583..38869
                     /gene="dctA"
                     /locus_tag="OHN28_000833"
     CDS             37583..38869
                     /gene="dctA"
                     /locus_tag="OHN28_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCQ2296273.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39089..40585
                     /locus_tag="OHN28_000834"
     CDS             39089..40585
                     /locus_tag="OHN28_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCQ2296274.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(40681..41610)
                     /gene="kdgK"
                     /locus_tag="OHN28_000835"
     CDS             complement(40681..41610)
                     /gene="kdgK"
                     /locus_tag="OHN28_000835"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCQ2296275.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41842..42609
                     /gene="pdeH"
                     /locus_tag="OHN28_000836"
     CDS             41842..42609
                     /gene="pdeH"
                     /locus_tag="OHN28_000836"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCQ2296276.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            42679..44739
                     /locus_tag="OHN28_000837"
     CDS             42679..44739
                     /locus_tag="OHN28_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2296277.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44918..46240)
                     /locus_tag="OHN28_000838"
     CDS             complement(44918..46240)
                     /locus_tag="OHN28_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCQ2296278.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46633..47646)
                     /gene="yhjD"
                     /locus_tag="OHN28_000839"
     CDS             complement(46633..47646)
                     /gene="yhjD"
                     /locus_tag="OHN28_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCQ2296279.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47694..48593)
                     /locus_tag="OHN28_000840"
     CDS             complement(47694..48593)
                     /locus_tag="OHN28_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2296280.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGS
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            49113..49715
                     /locus_tag="OHN28_000841"
     CDS             49113..49715
                     /locus_tag="OHN28_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2296281.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(49766..51415)
                     /locus_tag="OHN28_000842"
     CDS             complement(49766..51415)
                     /locus_tag="OHN28_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2296282.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            51820..53217
                     /gene="ccp"
                     /locus_tag="OHN28_000843"
     CDS             51820..53217
                     /gene="ccp"
                     /locus_tag="OHN28_000843"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCQ2296283.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
BASE COUNT        12751 a        13253 c        14414 g        12836 t
ORIGIN      
        1 ggcctttagc gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt
       61 aaccatcgtt ctggatagca gagaagtgta cgaacacgtc tttagagcca tcgtcaggag
      121 tgatgaagcc gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg
      181 acatagtgta ttacctttaa taattaattg tgcctttcgg cgatatggcg tgctttacag
      241 attttgaagc gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga
      301 cttgccttac tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca
      361 cgcattggtg ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg
      421 gtcctgttat caggaccgta aacagaaata aagtggataa aagcctattc catcaactgc
      481 ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc
      541 ttcacgcgtc tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg
      601 aaatcatcga tttttaatcc tgtccctttt cgtaactgct caatttcggt acaggacgtt
      661 gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg
      721 ctcaacagct catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg
      781 ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg aaaaacagac acagcggggt
      841 gtagtcaggg catactctga ctatgattcg attcagccag tcatttagcg tgacttatag
      901 ccacctaacg atctgataaa tgccgatata tctcttacca ctcccggttt gtaaagattt
      961 cgaaaatcta acgttctgcg aaggcgtaaa tctctggcta caaaaaaggc aacggcaata
     1021 tcatttttta acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca
     1081 ggaagattat gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag
     1141 acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga
     1201 aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt
     1261 gggtgttttg ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca
     1321 gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt
     1381 ccttttgtct ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg
     1441 gattccggtc tgcgcccggt tgtagacagc gttaacgcaa ttcgcgtacc gcaggattac
     1501 gttacgcaaa gcggtccgct gcgtgaaatg aacggttcgc tgggtgtgct ggcgcaacag
     1561 ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg cgttaaaaca gagtgatgac
     1621 ctgaaaccgg tctttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg
     1681 caaccgttga ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac
     1741 attgctcagc aaggtactca ggtgagcttt gtcgcaaatg gcatccagtt cccgacttca
     1801 cagcaggcaa gtgaatataa caaactgatt gcgccattac cggcacaaca tcaggcgttt
     1861 aatcaggcct ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt
     1921 atcgggcttt actcctcgca gtcgcggctt agtccgcgac gtgcggattc acacagttct
     1981 tctcaacctt tccttgtaac gcattaatca aattatccac ggcacaggcg gccatgccat
     2041 aacgcgtctc atgggtggca gatccaatat gcggtaatgc gacgacgttg gccattgaga
     2101 gcaacggcga atctacggac agtggctctt gttcgaagac atccagcccg gcggcgtgaa
     2161 tttccccttt ctgcaatgca gcaatcagtg cattttcatc aaccaccggg ccacgtccgg
     2221 cattaatgaa aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat
     2281 gcgtctcatc cgttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagagtat
     2341 ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cgacgcgcgt
     2401 tatagaggat gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc atgccgatcc
     2461 gtcccatccc gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc
     2521 ctatactcgc ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag
     2581 cggtagacaa caccagcgcc atcagcgtat cggcgacggt ttctgttaat acggttggtg
     2641 tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga
     2701 cggagatcgt tgatgtggca cgcagtttcg gcattttttc cagcaatgcg gcatcaacat
     2761 tctcatttga acccagcaag ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg
     2821 tttgtgggct gaggtttgct acctggtgaa cggtgaaatg ttcttgcagg cgttgcagta
     2881 aatcatcagg taaggctttg tagaggataa cggacggctt catgcttctc tccattcact
     2941 gataaggcga tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat
     3001 ttctgcatca cctgacatga aagggattac agtgggctta aggtaatttc tacacggcgg
     3061 ttttgcgcct taccttctgc ggtgctgttg ctggcgattg ggttagccgg gccaaggccc
     3121 tgagtacgga tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc
     3181 gcacgctgct gggagagacg catgttcagg tcgtgactcc ccgtgctgtc ggtataacca
     3241 atcacgttaa ccgccgtttt cggatactct ttcagtacca ttgccacgcc ggtcagggtg
     3301 ttagcgcccg ccggtttcag ggttgcgctg ctgctgtcga aggtcacatt gttcggcata
     3361 ttgaggataa tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca
     3421 cgcagcttcg cttcctgcac atccatgtaa taaccaacgc cgccgcccag agctgcgcct
     3481 gctgctgcgc caatgagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca
     3541 atacccgcgc caacgagaga gcccagacct gcgccgatag cagatttacc tgcttcgcgt
     3601 tcgccggtgt aagggttagt tgtacagcca gataccgcca gagcgccact cactacggcg
     3661 gcaataagat aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca
     3721 cgggttccgt ggcgggagat tatgccgcgt gagcatgaag attattcctg ggaatactcg
     3781 gaaatttgta agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt
     3841 ggccaactca tcctcacgat attccgttct gactgccgcc cactgggggc ctatgctggt
     3901 tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag gaatggaaaa
     3961 ctccttgcag agcgcggttc gcgaccaggt tcacagcaat acgcgggtgc gatttccaat
     4021 ggtgcgcaaa ggctttcttg cgtcaccgga aaatccgcaa ggcattcgtg ggcaggatga
     4081 atttgttcgc gtgagttggg atgaggcgct ggagcttatt caccatcaac ataaacgcat
     4141 tcgtgaggct tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg
     4201 cgtgctgcat aaggcctcga cattattaca acgctatatg gcgttggcag gcggttatac
     4261 tgggcatctg ggggattatt cgaccggcgc ggcacaggcg atcatgccgt atgtcgtggg
     4321 tggcagcgaa gtttatcaac agcaaaccag ttggccgctg gtgctggaac atagcgatgt
     4381 cgtggtgctg tggagtgcta acccactcaa tacgctgaaa attgcgtgga atgcatccga
     4441 tgagcagggg ctttcttact tttccgcact gcgtgacagc gggaaaaagc tgatctgcat
     4501 tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt gggtggcacc
     4561 gcacatgggc accgatgttg cgttgatgct ggggatcgcc catacgctgg tggaaaatgg
     4621 ttggcacgac gaagcgtttc tggcgcgttg caccacaggt tatgctgtct tcgcctctta
     4681 tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa tgggcggcag agatttgtgg
     4741 tgttggcgca gcgaaaatcc gcgagctggc ggctattttc caccaaaata caaccatgct
     4801 gatggcaggc tggggaatgc agcgccaaca gtttggtgag caaaaacact ggatgatcgt
     4861 cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta
     4921 ccattttgcc aatggtggta accccacgcg ccgcgctgcg gtgctctctt ccatgcaggg
     4981 cagcttgccg ggtggcaccg atgcggtaga taaaatccct gtcgcccgca ttgttgaagc
     5041 actggaaaac cctggtggcg catatcaaca taacggtatg gaccgacatt tcccggatat
     5101 tcgttttatc tggtgggcgg gtggtgccaa ctttactcat catcaggata ccaatcgcct
     5161 gatccgtgcc tggcaaaaac cggagctggt ggtgatctcc gaatgcttct ggacggctgc
     5221 ggcgaaacac gcggatatcg ttctgcctgc gaccacctct tttgagcgta atgatctcac
     5281 catgaccggt gattacagta atcagcatct ggtgccgatg aagcaagtgg tgccgccacg
     5341 cgatgaagcg cgtaatgatt ttgatgtttt tgccgagttg agtgaacgtt gggagaaggg
     5401 cggttatgca cgttttacgg aaggaaaaag tgagctgcaa tggctggaaa cgttttataa
     5461 cgttgcccgg cagcgcgggg caagccagca ggttgaattg ccgccatttg ctgagttctg
     5521 gcaagccaac cagttaattg agatgccgga aaacccggac agtgagcggt ttattcgctt
     5581 cgccgatttt cgccgcgatc cgcaggcgca tccattaaaa accgccagcg gtaagattga
     5641 aatcttctcg cagcgtattg ccgattacgc ttatccggat tgcccggggc atccaatgtg
     5701 gctggcgccg gacgaatggc agggtaatgc cgaaccggaa cagttgcagg tactttctgc
     5761 tcatccggcg catcgtctgc acagccagct gaattacagt tctctgcgcg aattgtacgc
     5821 ggtggcaaat cgtgagcctg tcaccattca tcctgacgat gcccagacgc gcggcataca
     5881 agatggcgat actgttcggc tgtggaactc acgcgggcaa attcttgccg gcgcggtcat
     5941 tagcgaggga attaaacctg gcgtgatttg tattcacgaa ggcgcatggc cggatttgga
     6001 tttaaccgct gacggcattt gtaaaaacgg cgcagtgaac gtgctgacca aagatctccc
     6061 cagctcgcgg ctggggaatg gctgtgcggg taatacggcg ctggcatggc tggaaaaata
     6121 caacggtccg gaactgacag ttacagcgtt tgaaccaccg gccagctcat aatccaggtg
     6181 ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt gaaaaccctg cgcatggtag
     6241 aaatctatcg ccggttgatt tttttgataa acctccagca tcagatgggg atggcgctgc
     6301 tgcacatact gcatcagcgc cttaccaata ccgcgcctga cggccttcgg tgcgacaaac
     6361 attgctgcca gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct
     6421 tcccagaccc agttttgcgc gttggcaaga tacgcatccc gcaccagcgg aatgcagtca
     6481 cgccagtaac tcgattttat aaagggatgc ccccaggttg tactttccag ccacagttcg
     6541 aggagcgcgg ggagttctga acgttgcgct tcccgaatca tggtttattt cccggatggc
     6601 aacagcagcc aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga
     6661 ttgtggtacc gacaaactta aaaccacgtt tttttagtgc cttagatagg gcgtcggagg
     6721 cgggtgtaga tgtggggatt tcgctcaacg ttgtggcttg tgtcacctgt ggttgatgat
     6781 tgacaaacga ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt tgcaggtacg
     6841 cccgcgcatt accaataatt gcctgaattt tcccacgatg gcggataatc ccggcgtcct
     6901 gtaccagtct ttcgacatcc tcttcctgca ttgcggcaac tttcaccgga tcgaactgat
     6961 gaaagcatgc gcgatagttt tcgcgttttt tgaggacggt gatccacgat aacccagcct
     7021 gctgcccttc caggcagatc atttcgaaca gttttttact gtcagtttca ggcacgcccc
     7081 attcattatc atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca
     7141 tactttccct cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg
     7201 tgaaaaagtc aatatttaat acagggctac tatgcccgat atccttcata caatgacaat
     7261 aatattgccg aattcggctc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg
     7321 gtcgctggca gctaagcctg cttgcgagcg tggtaatcag tgcctttttt ctcaacacag
     7381 cttacgcctg gcaacaagaa tatatcgttg atacgcaacc cggacattcc acagagcgtt
     7441 acacctggga tagcgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa
     7501 gtagccaaag ggcgctggga ctggaagtca atctggcgga agaaacccct gtggatgtga
     7561 ccagcagtat gagtatgggc tggaattttc ctttatatga gcaggttaca accggcccgg
     7621 tcgcggcatt acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta
     7681 ctaccacgct ggccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg
     7741 actcccggct tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg
     7801 agaatatctg gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca
     7861 actggctgga tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gctgcctatg
     7921 ctgcgctatc tcaggcagaa aataccacta acaatagcga ctatctgtat acgatggggg
     7981 ttagtgccag attttaacgt tatagataca attgaaaccc ataaataaca actaatgtgg
     8041 cgatagtggg gactaactta ctaaataata aatttggcga ataattgtcg agtcattcat
     8101 tcctgaacta atgcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca
     8161 taaagcgcac ttcgttatgg ttgtcagcag agatttttcc tttttattac tgcaggaata
     8221 ctgccatgac accttcaaat tatcagcgta ctcgctggct gacactcatc ggtactatca
     8281 ttacccagtt tgctctgggg tcggtttata cctggagtct gtttaacggt gcgctttctg
     8341 ccaagctggg ggctcctgta agccaggttg ctttctcttt cggcttgtta agtctggggc
     8401 tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg ttttggtgtt aaacgcgtca
     8461 ctatggcttc cggcattttg ctgggattag gtttcttcct gacagcacat tccaacaacc
     8521 tgatgatgct gtggttaagc gccggtgtgt tggttggtct ggcggatggt gcgggttacc
     8581 tgttgacgct ctctaactgc gtgaagtggt ttccggagcg taagggactt atctctgctt
     8641 ttgctatcgg ttcttatggt ctgggcagcc tgggttttaa atttatcgac acgcacctgc
     8701 tcgaaacggt gggtctggaa aaaacctttg ttatttgggg cgcgattgta ctggtgatga
     8761 ttgtctttgg cgcaacgtta atgaaagatg cgccgaagca ggaagtgaaa accagcaatg
     8821 gtgtggtgga gaaggactac accctggcag agtcgatgcg taaaccgcag tactggatgt
     8881 tagcggttat gttcctgact gcgtgcatga gtggtctgta tgtgattggt gtagcgaaag
     8941 atatcgctca aagtctggcg catcttgatg caatttccgc agccaatgct gtgacggtta
     9001 tttccatcgc caacctttct ggtcgtctgg tgctgggcat tctgtctgac aaaatcgccc
     9061 gtatccgtgt tatcaccatt ggtcaggtga ttgcgctggt gggtatggcg gccctgctgt
     9121 ttgcaccatt gaatgcagtg acgttctttg cagcgattgc ctgtgtggca tttaactttg
     9181 gtggcacgat tacggtgttc ccgtcactgg tcagtgagtt cttcggcctc aataacctgg
     9241 cgaaaaacta cggcgtcatt tacctcggtt tcggtatcgg cagcatttgt ggttcgatta
     9301 tcgcttcact gtttggcgga ttctatgtga ctttctacgt gattttcgcc ctgctgattc
     9361 tgtcactggc gctttctacg accattcgcc agccagagca gaaaatgttg cgtgaggcgc
     9421 atggctccct ttaatgctca gccgtgagct actgggcttc agcttcagtg tgcatcaaac
     9481 gcatcaacga aatgtataaa caataacgcg taaaatttgg gccagttaac tggccctttt
     9541 tactgctttt tgtaacgcta aagtctcttt tcaaacttgc atttttgtaa atttgtgctt
     9601 cgtgcacact ctttccccac actttttccc tttgctgtgg tctacttatt cgcgcgtgta
     9661 gattttactt atctgactac ctccgcactt ttcccctgcc tggcctgaaa agccactaag
     9721 cagggtgtta tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac
     9781 acagcagaag ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt
     9841 tttttaccgc cgctttggca gctatgcgca cgattttacc gtctggaaag gcatttctgc
     9901 tgttgttgaa ctggccgcca ccgtactggt gaccttcttt ttactgcgtc tgctttcgct
     9961 gtttggccgc cgcagctggc gtattctggc atcgctggtg gtgctctttt ctgcaggtgc
    10021 cagctattac atgaccttcc ttaatgtagt cattggttat ggcatcatcg catccgtcat
    10081 gaccaccgat atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat
    10141 cgccgtaagc gcgttgcctc ttatccttat ctggaataac cgctgtcgtt acaccttgct
    10201 ccgacaactg cgaaccccag ggcaacgtat tcgcagcctg gcggtcgtcg tactggcagg
    10261 tattatggtt tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc
    10321 gacaggtgtt gatttgccga gttatggcgg cgtcgtcgcg aactcttatc tgccatcaaa
    10381 ctggctttcg gcattaggtc tttatgcctg ggcgcgggtc gatgaatctt ccgataacaa
    10441 ttctctcctt aaccctgcga agaagtttac gtaccaggct ccacaaaacg ttgatgatac
    10501 atatgtcgtt ttcattattg gtgaaaccac gcgctgggat catatgggca ttttcggcta
    10561 tgaacgcaat accacaccga aactggctca ggagaaaaat ctggcggcgt tccgtggtta
    10621 ctcctgtgat actgcaacca aactctcact gcgttgcatg tttgtacgtc agggtggagc
    10681 agaagataat ccgcagcgca cattaaaaga acagaacatt ttcgcggtat tgaagcagtt
    10741 aggattcagt tctgatctct acgctatgca aagcgaaatg tggttctaca gcaatacgat
    10801 ggcggacaac attgcttatc gtgagcagat tggtgcggag ccacgtaacc gtggcaaacc
    10861 ggtagatgat atgttgctgg tagacgaaat gcagcaatcg ctggggcgca acccggatgg
    10921 taagcatctg atcattctgc ataccaaagg ctcacacttt aactacaccc agcgttaccc
    10981 gcgcagcttt gcgcagtgga agccggaatg tattggtgtt gatagcggct gtaccaaagc
    11041 gcagatgatc aactcatacg acaactcggt gacctatgtg gatcacttta tttccagcgt
    11101 gattgatcag gttcgcgata agaaagcgat tgtgttctat gcagctgacc acggtgagtc
    11161 aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa ctggcaccgc cggaacagtt
    11221 ccgtgtgccg atgatggtct ggatgtcgga taaatatctg gaaaatccgg tcaatgcgca
    11281 ggcatttgcg cagctgaaaa aagaagccga catgaaagtg ccacgccgtc atgtagagct
    11341 gtacgacacc atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa
    11401 caacaactgg tgccacatcc cgcaggcaag agaggcggcg gctaactaag aggcctgctg
    11461 actttctcgc cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc
    11521 agtaagatgc gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac
    11581 gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga
    11641 gcaggctttt ttgcatctgt aactcatgag gaagagaacc cccgggggcg gggaggttcg
    11701 actcgacgta ggcctgataa gacgcaccag cgtcgcatca ggcaaatgta ccggcccatt
    11761 gtcggatgcg gcgcgaccgc cttatccgat ctacacacca cacatttccc tgtcaaattt
    11821 gcgccccata tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat
    11881 atttgcttaa atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt
    11941 tttagcgttc atggtattaa tcgctcagat atccaccttt acctcagatt tttttcatta
    12001 aatgcataaa acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttgtgt
    12061 tggcgtagca caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt
    12121 gtggattctc accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt
    12181 gacagattgt aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta
    12241 attagtacgg catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc
    12301 aggcagcgac aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata
    12361 atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg
    12421 accgtcgcag caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa
    12481 gggtttaacc cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt
    12541 tataaccgtc tggttgaatt taaaatcggc accactgaag tcatcccggg cctcgctgaa
    12601 aagtgggaag tcagtgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag
    12661 tggcacgaca ataaagaatt caaaccgacg cgcgaactga atgccgatga tgtagtgttc
    12721 tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac
    12781 gaatacttcg aaggcatggg cttgccagag ctgatcagcg aagtgaaaaa ggtggacgat
    12841 aacaccgttc agtttgtgct gactcgcccg gaagcgccgt tcctcgctga cctggcaatg
    12901 gacttcgcct ctattctgtc aaaagaatat gctgacgcga tgatgaaagc cggtacaccg
    12961 gaaaaactgg acctcaaccc aatcggtacc ggcccgttcc agttacagca gtaccagaaa
    13021 gattcccgta ttcgctataa agcgtttgat ggctactggg gcaccaaacc gaagatcgat
    13081 acgctggtct tctctattac tcctgacgct tccgtgcgtt acgcgaaatt gcagaagaac
    13141 gaatgccagg tgatgccgta cccgaacccg gcagatatcg cccgcatgaa gcaggataaa
    13201 tccatcaacc tgatggaaat gccggggctg aacgtcggtt atctctcgta taacgtgcag
    13261 aaaaaaccac tggatgacgt gaaagttcgc caggctctga cctacgcggt gaacaaagac
    13321 gccatcatca aagcggttta tcagggcgcg ggcgtatcag cgaaaaacct gatcccgcca
    13381 accatgtggg gctataacga cgacgttcag gactacactt acgatcctga aaaagcgaaa
    13441 gccttgctga aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcaatgccg
    13501 gtacaacgtc cgtataaccc gaatgctcgc cgcatggcgg agatgattca ggcagactgg
    13561 gcgaaagtgg gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt
    13621 gcgaaagatg gcgagcatca gacggtaatg atgggctgga ctggcgataa cggggatccg
    13681 gataacttct tcgccaccct gttcagctgc gccgcctctg aacaaggctc caactactca
    13741 aaatggtgct acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccac
    13801 aataaacgtg ttgaactgta caaacaagca caggtcgtga tgcacgatca ggctccggca
    13861 ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca aagaagttaa aggctatgtg
    13921 gttgatccat taggcaaaca tcacttcgaa aatgtctcta tcgaataatt aaagccatac
    13981 aagactgatg gcaaaggcaa aactgcctga tgcgctccgc ttatcaggcc tacgaaaact
    14041 ctgcaatgta ttgagtttgc acgattttgt aggtcggata aggcgtttgc gccgcatccg
    14101 gcataaacaa agcgctcttt gtcaacaatc tgcatacccg gtggcgctgt gcctctgcgt
    14161 gcagcgccat ccggcagcaa tacttcattc cctgtcccac aagggcggga attgatttgt
    14221 gagcaataca gacacgcagt tccaggctgc gggtcactat agagaatccg ggttatgttg
    14281 cagtttattc tccgacgttt gggactcgtc atccccacgt ttatcggtat tacccttctc
    14341 acatttgcct ttgtccacat gatcccgggc gatccggtga tgatcatggc gggcgaacgt
    14401 gggatctccc cagagcgtca cgcgcaattg ctggctgaac tcggcttaga taaaccgatg
    14461 tggcagcagt atctccatta catttggggc gtaatgcacg gtgatttagg catttccatg
    14521 aaaagccgaa ttccggtatg ggaagagttc gtgccgcgct ttcaggccac gctggaactt
    14581 ggcgtctgcg cgatgatttt tgcgaccgca gtcgggattc cggttggtgt gctggctgcg
    14641 gttaaacgtg gttccatttt cgatcacaca gcggttggcc tggcgctgac cggctactcg
    14701 atgccaattt tctggtgggg catgatgttg atcatgctgg tttcggtgca ctggaacctg
    14761 acgcctgtct ccggtcgcgt gagcgacatg gtgttcctcg atgattccaa tccgttaacc
    14821 ggttttatgc tgatcgacac cgccatctgg ggtgaagacg gtaactttat cgatgccgtc
    14881 gcccatatga tcctgcccgc cattgtgctg ggtactattc cgctggcggt cattgtgcgt
    14941 atgacgcgct cctcgatgct ggaagtgctg ggcgaagatt atatccgcac cgcgcgtgcc
    15001 aaaggtctta cccggatgcg ggtgattatc gtccatgcgc tgcgtaacgc gatgctgccg
    15061 gtggtgaccg ttatcggcct gcaggtggga acattgctgg cgggggcgat tctgactgaa
    15121 accatcttct cgtggccggg tctggggcgc tggttgattg acgcactgca acgccgcgat
    15181 tatccggtag tgcagggcgg cgtattgctg gtggcgacga tgattatcct cgtcaacctg
    15241 ctggtcgatc tgctgtacgg cgtggtgaac ccgcgtattc gtcataagaa gtaagggggc
    15301 atcatgtcac aggttactga aaataaagtg attagcgcac cggtgccgat gaccccgtta
    15361 caggagttct ggcactattt taaacgcaac aaaggcgcgg ttgtcgggct ggtttacgtc
    15421 gtcatcgtgc tgtttatcgc gatctttgcc aactggattg caccctataa cccagcggaa
    15481 cagttccgcg atgcactgct tgccccgcct gcctggcagg aaggcggcag catggcgcac
    15541 ttgctgggca ctgatgacgt ggggcgtgat gtgctgtcgc ggctgatgta cggcgcgcgc
    15601 ttgtcgctgc tggttggctg tctggtagtg gtgttatcgc tgattatggg cgttattctc
    15661 ggcctgatcg ctggttactt tggcggtctg gtcgataaca tcatcatgcg cgtggtcgat
    15721 atcatgctgg cgctgccaag tctactgctg gcactggtgc tggtggcgat tttcggcccg
    15781 tcgattggta acgccgcgct ggcgctaacc ttcgttgcgc tgccgcatta tgtgcgctta
    15841 acccgcgccg ccgtgctggt ggaagttaac cgcgattacg tcaccgcgtc tcgcgtggcg
    15901 ggtgccgggg caatgcgcca gatgtttatt aacatcttcc cgaactgcct tgcgccgctg
    15961 attgttcagg cgtcgctcgg tttctctaac gccattctcg atatggctgc tcttggtttc
    16021 ctcggcatgg gcgcgcagcc gccaacgccg gagtggggca ccatgctctc tgacgtgttg
    16081 cagttcgcgc aaagcgcctg gtgggtcgtg accttcccgg gtctggcgat cctgctgacg
    16141 gtgctggcat ttaacctgat gggtgacggt ctgcgtgacg cgctcgatcc caaactgaag
    16201 cagtaagagg ttcgagatgg cgttattaaa tgtagataaa ttatcggtgc atttcggcga
    16261 cgaaagcgca ccgttccgcg ctgtagaccg catcagctac agcgtaaaac agggtgaagt
    16321 ggtcgggatt gtgggtgagt ccggctctgg taaatcggtc agttcgctgg cgattatggg
    16381 gctgattgat tatccgggcc gcgtaatggc ggaaaagctg gagtttaacg gtcaggattt
    16441 gcagcgtatc tcagaaaaag agcgccgcaa cctggtgggt gccgaagtgg caatgatctt
    16501 ccaggacccg atgaccagcc ttaacccgtg ctacaccgtg ggtttccaga ttatggaagc
    16561 gattaaggtg catcaaggcg gcaacaaaag tacccgccgt cagcgagcga ttgacctgct
    16621 gaatctggtc ggtattcccg atccggcatc gcgtctggat gtttacccgc atcagctttc
    16681 cggcggcatg agccagcgcg tgatgatcgc catggcgatt gcctgtcggc caaaactgct
    16741 gattgccgat gaaccaacca ctgcgctgga cgtgaccatt caggcgcaaa ttatcgaact
    16801 gctgctggag ctacagcaga aagagaacat ggcgctggtg ttaattaccc atgacctggc
    16861 gctggtggcg gaagcggcac ataaaattat cgtgatgtat gcaggccagg tagtagaaac
    16921 gggcgatgcg cacgctatct tccatgcgcc gcgtcacccg tatactcagg cattgctgcg
    16981 tgcgctgccg gaatttgctc aggacaaaga acgtctggcg tcgttgccag gtgtcgttcc
    17041 cggcaagtac gaccgcccga acggctgcct gcttaacccg cgctgcccct acgccactga
    17101 caaatgccgt gctgaagaac cggcgctgaa tatgctcgct gacgggcgtc agtccaaatg
    17161 ccattaccca cttgatgatg ccgggaggcc gacactatga gtacgcatga ggccaccccg
    17221 caacaaccgc tgttgcaggc tatcgacctg aaaaaacatt atccggtgaa gaaaggcatg
    17281 ttcgcgccgg aacgtctggt gaaggcgctg gatggcgttt cgtttaacct tgaacgtggc
    17341 aaaacgctgg cagtagtagg tgaatctggc tgcggtaaat cgaccctagg tcggttgctg
    17401 acgatgattg aaacgcccac cggcggcgaa ctgtattacc aggggcagga tctgctcaag
    17461 cacgatccgc aggcgcagaa gctacgtcgg cagaaaatcc agattgtctt ccagaaccct
    17521 tacggttcgc tgaatccgcg taaaaaagtt gggcagattc tcgaagagcc gctgctgatc
    17581 aacaccagct taagcaaaga acagcgtcgg gaaaaagccc tgtcgatgat ggcgaaagtc
    17641 ggcctgaaaa ccgagcatta tgaccgctat ccgcatatgt tctccggcgg tcagcgtcag
    17701 cgtatcgcca ttgcccgagg tctgatgctc gacccggatg tggtgattgc cgatgagccg
    17761 gtttccgcgc tggacgtctc agtgcgtgcg caggtgctga atctgatgat ggatttgcag
    17821 caggaactgg ggctatctta tgtctttatc tcgcacgatc tgtcggtggt ggagcacatt
    17881 gctgatgaag tgatggtgat gtacctgggc cgctgcgtgg agaaggggac gaaggaccaa
    17941 atcttcaata acccgcgcca cccgtacact caggcgctgc tctccgcgac accacgcctg
    18001 aacccggacg atcgtcgcga acgcatcaaa cttaccggtg aactgccaag cccgctgaat
    18061 ccaccgccag gctgcgcctt caacgcccgc tgtcgtcggc gcttcggccc ctgcatccag
    18121 ttgcagccgc agctaaaaga ctacggcggt caactggtag cttgttttgc cgttgatcag
    18181 gatgaaaatc cgcagcgtta actcacaaaa ccggggccat gcgctccggt ttactcaatc
    18241 aacttcaaca gtggcgacaa gcataacaaa atgccgtaca gcagaatcat ccaggcttta
    18301 aacccgcgaa gtttttccag ttgtgagact ttatagatca ggaaaaacgg aatgaggcac
    18361 gacacaatgc catataacgg gcttcccaac tgaaagaaca ccagtaccga tacacgaaac
    18421 gaaacccaaa tcgtcaacgt aatgacgata aaagtgcaga tcgccagagt cagcacgcgt
    18481 gagttaattt tcctggtatc aataattcgg cttaacagat tgagaataat gcctttaatg
    18541 gcctcgtgga aaccgagata aatgccaaag aatgcagtca gtacggcaaa gatattcagc
    18601 accgtagagg tgatatgaat gatatgccca gggatcacct gcgcggccag tgccagtgcc
    18661 gagatatttt gttcaaaggc agaaacggct tcttcgtggc taattgagaa ggtaaacgaa
    18721 aaggcaaaaa acaggatcac cgcgatgagc gtgacataac taattcggtg ggtacgcagc
    18781 gcgagccggg ttgccagtac tttatccgct tcccgtttac gataggcaat attcattggg
    18841 ttaagtacct gaataaatac cgcggaaaag aagcaaaatg gaatagtaag caggacatcg
    18901 cggaaaaagt cggaggcttg cgggaaggcg gttatattgg cgaaattcca gtgcgggatc
    18961 atcgcaaaac cgaacacgac aataatccct actttgacca ccaccattgg tccggaaatc
    19021 ttaaacagta atcgttcacc accagacgca atcgccacca gtacggcgaa aatggcgact
    19081 ttataaaata gagattgaga aagatcggca tcggttaaac cgaaggtttt caggtacgag
    19141 gcgctgtcga aaaccacgga gagagagtag ataaaaatcc cgtggataat catcaaaaag
    19201 tagataaccc cgaggaaaat tccccagttc ttccccaggt aatgactgat aatatcggtg
    19261 tagtcattac aggaatcgct ttcagaaagg gtttttaaat aaatgtcctg tactacccag
    19321 gtggcaggat aagcaatgat cgctgcggta ataaataccc aaattccctt caagccaatt
    19381 tgtactggca tcagcacggt tccggcacca atcgccatgc ctatgcataa taaaacccag
    19441 ccgaagtcgt agcgtgtaaa cggcaatttc tgatcgtgtt tcggtagtgt gttgtgctgc
    19501 ataaagtcct gtccagaaag aagtcggcct cgcaaaaatg aataaaccca gtttctcttg
    19561 cgcaacaggt ggagaagtcg aatacgcagt atgtaaaaaa tgaggcaaca gattcactgg
    19621 ggaggtcgcg aggaggcgca attctacaaa attgcgcatt tcactactag tctggagtca
    19681 gcccggatat gagctgcaac tattgcaaat aaagaaatta agctggaagg catcacgcaa
    19741 tgaggaagat tgccgggtgc tgacggcaaa aagcaatcgt aaaatcagcg gcaagccgga
    19801 agggttccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
    19861 gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gccattcttg
    19921 catctagacc attcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg
    19981 acgctcgcag agctgggcat ggccttctgg catgatttag cggctccggt cattgctggc
    20041 attcttgcca gtatgatcgt gaactggctg aacaagcgga agtaacgtgt catgcgggcg
    20101 tcaggctgcc gtaatggcaa tttgcgcccg gaccaggccg cagaggtgaa actctgcggc
    20161 ctttttcgta agaaaaatgc aacggttgca agcggataat attctgcgga atgcgcagca
    20221 cagagtgaag aagtgataga ttccggaaca gaaaaacagt cgtagaatca gcgtcaagcc
    20281 ggaagggttc cggtgaggcg caatgttgcg ggggcttaat ccctggtggc attggttgct
    20341 ggagagagaa aacccccgca cgttgcaggt atgcacctga caacatcacg ggggctaatc
    20401 ttgaacctga acaactcaag aatagccgcg aaacgttgtc attacaacac aggcggctat
    20461 atgacgctcg cagagctggg catggccttc tggcatgatt tagcggctcc ggtcattgct
    20521 ggcattcttg ccagtatgat cgtgagctgg ctgaacaagc ggaagtaacg tgtcatgcgg
    20581 gcgtcaggct gccgtaatgg caatttgcgc ccggaccagg ccgcagaggt gaaactctgc
    20641 ggcctttttc gtaagaaaaa tgcaacggtt gcaagcggat aatattctgc ggaatgcgca
    20701 gcacagagtg aagaagtgac ggattccgga gcagaaaaac agtcgtagaa tcagcggcaa
    20761 gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt
    20821 gctggagaga gaaaaccccc gtacgttgca ggtatgcacc tgacaacatc acgggggcta
    20881 atcttgaacc ttaacaactc aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc
    20941 tatatgacgc tcgcagagct gggcatggcc ttctggcatg atttagcggc tccggtcatt
    21001 gctggcattc ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgtcatg
    21061 cgggcgtcag gctgccgtaa tggcaatttg cgcccggacc aggccgcagg gggaaactct
    21121 gcggcctttt tcgttcttac tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt
    21181 acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc ggtgctgttt
    21241 gtggtaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa atcttgccat
    21301 caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg tcgatgacaa
    21361 tcggagcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg gggacgtctg
    21421 tgatagacgg gctagggata tcacgtagac cagatacctg cattctgtcg cccttcagcg
    21481 cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc tccagttcag
    21541 taaagaaggc gtccagttca tcaaagaatt tttgcgcccg cgctttgtaa tccgctgttt
    21601 tgctgacgcc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac
    21661 tacggctgtt tttatctttt tcggtaacgt ccagccaacg gttaagcacg gcggtatcgt
    21721 cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt tgatccatca
    21781 attcgctctg catgccgcca ttttcgcgaa cttccttcaa aaaaccaccg aactggccgt
    21841 tatgtcccat catcaggtgc tgggtaaagc ccagtttcga caggttatca aacagatagc
    21901 agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca
    21961 gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc ttgaactcaa
    22021 tatcgaaatg cgaccacagt ggatgcgaca tcaatccggc ggcttcaata tccgaccagg
    22081 aaagcgaaca gatattaatc accagtagtt caaatggctg agcatcagcg ggcagcgaag
    22141 acgggaagat cgatttacgt ttcgcctccg cgttatagaa attattcagc caggcgttaa
    22201 ggtttgctgt tgttggcggt gcagtttgtg cgggcatatc acccactatc ggtgcgccac
    22261 ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg gtcgttggtt
    22321 gaccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc agccatagca
    22381 gaatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac caggccacga
    22441 ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca agatcgatta
    22501 aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt tccggaccag
    22561 gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc cagtggcgca
    22621 agcgatgcag gctatagtgc ggaatgggca tcagtagaaa cgcggcaaac accaaattga
    22681 ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc agaaaataga
    22741 agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg gcggtatttt
    22801 gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta acgtcccggc
    22861 cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa
    22921 agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga caataatttc
    22981 aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa cagtcgcatt
    23041 ggttctggta ttcgtcgcca gtggcgacca tcatgctcgg cattgatgac cggtttagaa
    23101 ctttgcgcta aaggcagcgg catgccccat tgttctgggg caagcaggcg catctgcacc
    23161 agctcggcac tgatttgatc atcttcaaac cagaccattc ggtttgagaa aatgtcgcca
    23221 gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg acagaatgag
    23281 agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg gttagggcga
    23341 catagcgtca gggcttgctc aacgcggata cccggtaccg gacgtagggc aaccagaacg
    23401 cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc attacagaac
    23461 acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag caaggtagtg
    23521 atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat catcgtcaga
    23581 cagcgggaga gtggcgcatt ccacgggata accatatttg caccgcaggc caataataaa
    23641 cgttcatcgg tggcgcgcag gctggcggta ttttcccgta cgaggatttt catcgcacta
    23701 ccgcgctggc gacgcagagt atgaatgctg cgggccagtg gctcgatttg cgcattttgc
    23761 tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa caggacttcg
    23821 ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag tacagcaaca
    23881 ttactcagaa tgcgtttttc gtcgctgcgt ggttggattt ccgcctcttg gtgttgaact
    23941 aatgtccagc aaccattttg ttgctgaacg ctaagttgct gacgggcgct gacccctttt
    24001 tcgttgcacc agaaagcaat atccagcaaa tgttggtcgc cctgaaaacg caaactggca
    24061 agaccaaaaa gtgaccgata ttcctcaagt aacaatgaaa attgtttatc gttattattt
    24121 ccggaattaa ttaccaaaag cgaacaatgg tttagccggc tccatttatt cattttatcc
    24181 aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cattatttgc gcaaacaaga
    24241 ataaaaaggt aattatgggg atcaatagaa cactgcaaat cacgggccaa atagtataga
    24301 cctttttcat gatttagcat cgaaaataat gttatttttt ccggcccttg agaatcatct
    24361 aattgaaaga ttttcgctgg atcgctgtcc atgctaatga ccgcgacgtg tgcggtttca
    24421 gcctgggatg caattgtttg attcgccaga ctgatagcat cttcatggcg atcgacgtta
    24481 aaccaccaga cgccgcctgc tggcatatgt cgcagctcat cccataatga tgagataccg
    24541 atagagaata cagggtccac aatgtccctc atgtcaacat tttgtctgat tatcagttta
    24601 ctagcgaaag ctaagaaata aacctaccat tgaaattaaa acttatcaga tttagcatgt
    24661 gaatcaattt agcaggtcac aagacatgtg attttagtct tttcgttttt aactttttct
    24721 gttgcataac ggaaagtcaa aaagtgagca cattcccgtc tcgccgtaaa caataaaccg
    24781 acgaaatgct cacagttaga attgtctcat gaacggtacg gttatttcat agggatcaag
    24841 aaaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct atatcttcca
    24901 gaatgatatt ttggcgttaa agcaggcatt ttcactgcct gatattgatt atgccgatat
    24961 ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg cagagtttgc
    25021 gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt tcggggaggc
    25081 gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt gggagaaaat
    25141 gtcctggtgg tcgatgccag cccggacaac ttgttgcgcc tgtcatttaa cgttgatttt
    25201 acccaccgtc agggttgggc cagagctatg ctggatggcc aggactggcg tgacgccggg
    25261 ttgcgctata cctcgcagct cgatttgctg ccttttggtc agttatcgat tgaagaacaa
    25321 gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt acagcaacta
    25381 aaagccagcg ggcgttacca gtggatttta attgacttac cgcgtgatgc ctcgcagata
    25441 acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt ggatgccaac
    25501 tgccatatcc gactgcatca gcaagcgctg ccggatggtg cacatatttt gattaatgac
    25561 ttccgtattg gcagccaggt tcaggacgat atttaccagc tttggttgca aagccagcgc
    25621 cgattgctgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgtct ggcagctaag
    25681 caaccagtcg gtgaatatcg cagtgatgcg ctggcggctg aagagatact gacgctggcg
    25741 aactggtgcc tgttgaatta ttccgggctg aaaacgccag tcgggagtgc atcatgagta
    25801 tcctgacccg gtggttgctt atcccgccgg tcaacgcacg gcttattggg cgttatcgcg
    25861 attatcgtcg tcacggcgcg tcggctttca gcgcgacgct cggctgtttc tggatgatcc
    25921 tggcctggat ttttattccg ctggagcacc cgcgctggca gcgtattcgc gcagaacata
    25981 aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc
    26041 ttattcaaac atgctggtta ctgatcggtg catcgcgcaa agaaacgccg aaaccgcgca
    26101 ggcgggcatt ttcaggtcta caaaatattc gtggacgtta ccatcaatgg atgaatgagc
    26161 tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag ctcggtcatt
    26221 tgagtgccgg gacgcggcgg ttgatcctcg gtatcatcgt caccttctcg ctgattctgg
    26281 cgttaatctg cgttactcag ccgtttaacc cgctagcgca gtttatcttc ctgatgctgc
    26341 tgtggggggt agcgctgatc gtacggcgga tgccggggcg cttctcggcg ctgatgctga
    26401 ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc tcgacgttaa
    26461 actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg
    26521 cgtggattgt gctggtgctc ggctatttcc aggtcgtatg gccgctgaat cgtcagccgg
    26581 tgccattgcc gaaagatatg tcgctgtggc cgtcggtgga tatctttgtc ccgacttaca
    26641 acgaagatct caacgtggtg aaaaatacca tttacgcttc gctgggtatc gactggccga
    26701 aagacaagct gaacatctgg atcctcgatg acggcggcag ggaagagttt cgccagtttg
    26761 cgcaaaacgt gggggtgaaa tatatcgccc gtaccactca tgaacatgcg aaagcgggca
    26821 acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgatattc gactgcgacc
    26881 acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc
    26941 tggcgatgat gcagacgccg caccacttct tctcgccgga tccgtttgaa cgcaacctgg
    27001 ggcgttttcg taaaacgccg aacgaaggca cgctgtttta tggtctggtg caggacggca
    27061 atgatatgtg ggatgccact ttcttctgcg gttcatgtgc ggtgattcgc cgtaagccgc
    27121 tggatgagat tggcggtatt gctgtcgaaa cggtaaccga agatgcgcac acttctctgc
    27181 gtctgcaccg tcgtggctac acctcagcgt atatgcgtat tccgcaggca gcggggctgg
    27241 cgaccgaaag tctctcggcg catatcggtc agcgtattcg ctgggcgcgc gggatggtgc
    27301 aaatcttccg tctcgataac ccgctcaccg gtaaagggct gaagtttgct cagcggctgt
    27361 gctatgtcaa cgccatgttc cacttcttgt cgggcattcc acgactgatc ttcctgactg
    27421 cgccgctggc gttcctgctg tttcatgcct acatcatcta cgcgccagcg ttgatgatcg
    27481 ccctgttcgt gctaccgcat atgatccatg ccagcctgac taactcgaag atccagggca
    27541 aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg tatatcgcac
    27601 caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc accgccaaag
    27661 gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac atcttccttg
    27721 ttctgctcaa cctggtgggc gtagcggttg ggatctggcg ctacatctat ggtccgccaa
    27781 ccgagatgct caccgtggtc gtcagtatgg tgtgggtatt ctacaacctg attattctcg
    27841 gcggcgcagt cgcagtatcg gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga
    27901 tgacgatgcc cgcagcaatt gcccgcgaag atggtcacct cttctcgtgt accgttcagg
    27961 atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt ctggaagggc
    28021 agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg gctcaggtgg
    28081 cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcactacc cagcaacata
    28141 tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg caggacagct
    28201 acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc ttccgtggct
    28261 accgccatct ggcggaattt gcgccttctt cggtgaaggg catattccgt gtgctgactt
    28321 ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc gaaacggcac
    28381 aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag aaaaatattc
    28441 tggatttgtg cagtggctct ggggatgagt gcgttcccct ctttcatgac gcaggcgacg
    28501 ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccaggc ggagcaaaat
    28561 ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgcag atgcgccagt cgtggcgcag
    28621 aacggtcctt cccgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc gccgggcagc
    28681 atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg cagcgatgaa
    28741 gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcatt actgcctgtc
    28801 cagtcgcagt taaaggttta tctcaatgat gaactgatgg gcgtgctgcc agtgaccaaa
    28861 gaacagttgg gtaaaaaaac gctggcacaa atgcccatta acccgctgtt tattactgat
    28921 ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga aaacccggcc
    28981 agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac ctatcagacc
    29041 ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgaccc gcgtgataac
    29101 cgcaccaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgagct gcaacaagca
    29161 tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca gaacttcccg
    29221 gtgctctata accaactgcc ggatcgcaat gccattgtct ttgccaccaa tgacaaacgc
    29281 ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat gattaaccat
    29341 ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa agacctgttg
    29401 caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag tgtggtagtg
    29461 aatgaagtga aaccgctgct accgcgtaaa ccgtacgatg cgccgaactg ggtgcgtacc
    29521 gatcgtcctg tcacctttgg tgaactgaaa acctatgaag aacaattaca atccagcggt
    29581 cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta cctgatgcgc
    29641 agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt gaaagacagt
    29701 tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct gagcagcaaa
    29761 caggaggcga accgcctgct gctgcgtatt ccggtattac aaggtttgct ggatggcaaa
    29821 acggatgtct ctattccggc gctgaaactg ggcgcgacca accagttgcg gttcgacttt
    29881 gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt ccagccggtg
    29941 cagaatcatg tggtgattgg tgacgactcc accatcgact tctcgaagta ttaccacttc
    30001 atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag ccggatggcg
    30061 gatttgtcgc aaaccatcac cgtgatgccg aaaacgccaa acgaagcaca gatggaaacc
    30121 ttgctgaata ccgttggctt tatcggtgcg cagacgggct tcccggcgat taacctgacg
    30181 gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat cgtcggtggt
    30241 atcccggaca aactgaaaga cgataagcag atcgacctgt tggtgcaggc gaccgaaagc
    30301 tgggtgaaaa cgccgatgcg ccagaccccg ttccccggta ttgtgccgga cgagagcgat
    30361 cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc ggtgattggc
    30421 ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga tagcccacgc
    30481 ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac catgttcggt
    30541 tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga tgtttattac
    30601 gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg
    30661 ctggcggtgc tggcggctat cagtgtgata ttgctggcgt gggtactgtg gcgtctgctg
    30721 cgtattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat gaatgtgttg
    30781 cgtagtggac tcgtgacgat gctgctgctg gctgccttta gtgttcaggc agcctgtacc
    30841 tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg cgtcatcgac
    30901 cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggcat gttctttgcc
    30961 ctggcggcta acgaccgtgc agctttcgat aatcttctcg actggacgca gaacaatctc
    31021 gctcagggtt ctttaaaaga aaatttgccc gcctggctgt ggggcaagaa agagaacagt
    31081 aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat ggcctggtcg
    31141 ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg cagcgcattg
    31201 ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggctc catgttgtta
    31261 ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag ctacctgccg
    31321 ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc
    31381 aatcaacgtt tattgctgga aaccgccccg aaaggctttt cgccagactg ggtgcgctat
    31441 gagaaagaca aaggctggca gctaaaagcc gaaaaaacgt tgatcagcag ctacgacgct
    31501 atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa agcgcggatg
    31561 ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc gccggaaaaa
    31621 gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc tgccgccatg
    31681 ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt ggccgataac
    31741 tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca aggctgggat
    31801 caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg ccaggaatgc
    31861 gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg
    31921 aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc gaagcgaccc
    31981 atcgtgaaga cctggtgcaa cagtcgttgt atcggctgga acttattgat ccgaataacc
    32041 cggatgtgat tgctgcccgt tttcgttctc tgttacgtca gggcgatatt gatggcgcac
    32101 aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat aaatcgtcgc
    32161 ggactacgat gctgctttcc acgccggatg gtcgtcaggc actgcaacag gcaagattgc
    32221 aggcaactac cggtcatgcg gaagaagctg tggcgagtta caacaaactg ttcaacggtg
    32281 cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa attccggctc
    32341 gccgtggcga agcgattaat cagctaaagc gcattaatgc ggatacgccg ggcaacacgg
    32401 gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac gaaggctttg
    32461 ccgtcctgga acaaatggcg aaatcgaacg ccgggcgcga aggggcctct aaaatctggt
    32521 acgggcagat taaagacatg cccgtcagcg atgccagcgt gcaggcactg aaaaaatatc
    32581 tctcgatctt tagcgatggc gatagcgtgg ctgctgcgca atcgcaactg gcagaacagc
    32641 aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg gcggtggact
    32701 ctggcatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg aacccgaaag
    32761 acagtgaggc tctgggggcg ctgggccagg cgtattccca gaaaggtgat cgcgccaatg
    32821 cagtggcgaa tctggaaaaa gccctcgcgc tggatccgca caacagcaat aacgacaaat
    32881 ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc gatgccgcgc
    32941 tgaaagccaa taatcctgac cgggcagaac ggctgttcca gcaggcacgt aatgtcgata
    33001 acaccgacag ctatgcagtg cttgggctgg gcgatgtggc gatggcgcgc aaagattatc
    33061 ccgccgccga acgctattac cagcagacct tgcgtatgga cagcggcaac actaacgccg
    33121 tgcgcgggct ggcgaatatt taccgccagc agtcgccaga aaaagccgaa gcgtatatcg
    33181 catcgctctc tgccagccag cggcgcagca ttgatgatat cgaacgcagc ctgcaaaacg
    33241 accgtctggc acagcaggca gaggcactgg aaaaccaggg gaaatgggcg caggcggcag
    33301 cacttcagcg gcaacgactg gcgctggacc ccggcagtgt atggattact taccgacttt
    33361 cgcaggatct ctggcaagcc ggacaacgca gccaggccga tacgttaatg cgcaatctgg
    33421 cgcagcagaa gccgaacgac ccggagcagg tttacgctta cgggctgtat ctctccggtc
    33481 acgaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg cagtggaaca
    33541 gcaatattca ggagctggtt aatcgactgc aaaacgatca ggtgctggaa accgctaatc
    33601 gcctgcgaga aaacggtaaa gaggcagaag cggaagcgct gctgcgccag caaccgcctt
    33661 cctcgcgtat tgacctcacg ctggctgact gggcgcagca gcgacgtgat tacaccgccg
    33721 cccgcgcggc ttatcagaac gttttgacgc gcgagcccac taacgccgat gccattcttg
    33781 gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc cagctggcga
    33841 aactgcctgc cactgataac gcctcgctga acacacagcg gcgcgtggcg ctggcacagg
    33901 cgcagcttgg cgataccgca gcggcgcagc agacatttaa taagttgatc ccgcaggcaa
    33961 aatctcagcc accgtcgatg gaaagcgcga tggtactgcg tgatggtgcg aagtttgaag
    34021 cgcaggcggg cgatccaatg caggcgctgg aaacctacaa agacgccatg gttgcatccg
    34081 gtgtgaccac gacgcgtccg caggataacg acacctttac ccggctgacc cgtaacgacg
    34141 agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc tatcgccagc
    34201 aggatcttaa cgtcactctc gagcacgatt actggggttc gagcggcacc ggtggttact
    34261 ccgatctgaa agcgcacacc actatgttgc aggtggacgc gccctattct gacgggcgga
    34321 tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact aatgccgatg
    34381 gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc aaccgcagcc
    34441 agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg agctgggata
    34501 tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc agttacagcg
    34561 atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcctatc tccagttctt
    34621 tgctggcctt tggtgggcaa aaagactctc cgagcaatac cgggaaaaaa tggggcggcg
    34681 tgcgtgccga cggcgtgggg ctaagtctga gctacgataa aggtgaagca aacggcgtct
    34741 gggcatcgct tagcggtgat cagttaaccg gtaaaaatgt cgaagataac tggcgcgtgc
    34801 gctggatgac gggctattac tataaggtca tcaaccagaa caatcgccgc gtcacaatcg
    34861 gcctgaacaa catgatctgg cattacgaca aagacctgag tggctactca ctcggtcagg
    34921 gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg tggcgggagc
    34981 gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat tcacgcacca
    35041 aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag gaagaagctg
    35101 cgcggcaatc caacgatggc ggcagcagtc agggctttgg ctacacggcg cgggcattac
    35161 ttgaacgacg tgttacttcc aactggtttg tcggcacggc aattgatatc cagcaggcaa
    35221 aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc ggatggcagg
    35281 gtgacatgga tttaccaccg cagccgctga taccttacgc cgactggtaa gttttcagat
    35341 agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg ggatttccgg
    35401 ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca atggtggcag
    35461 ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat ctggtccagc
    35521 agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc cgcgaaccct
    35581 tatcttctgc cattttgaaa ggcgatattc ccgaagcgga agctattctt gccagcatta
    35641 aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc caaccagttc caggcgctgc
    35701 gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg tttgagctac
    35761 cggtgcaaat ctcgctgggc gtttactcgc tggaacgtcc ggcaaacccg cagccaattg
    35821 cctatctggt gctacaggcg gattccttcc gcatgtataa gttcgtgatg agcaccctct
    35881 caacgttagt gaccatttac ttacttttgt cgttaatatt gactgtggcg attagctggt
    35941 gcattaaccg cctgattttg catccattac gcaatattgc tcgcgaactt aacgccatcc
    36001 caccccagga tcttgttggt caccaactgg cattaccgcg tctgcatcag gacgatgaaa
    36061 tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc cattatgaag
    36121 aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa gccttgctga
    36181 tggagatgct ggagcaggtg gtggcgcgta aacaaaccac cgcgctgatg atcatcacct
    36241 gtgaaaccct gcgtgatact gcgggtgtgc tgaaagaggc gcaacgagaa attctgctgc
    36301 tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg catgatcctc gcgcagatta
    36361 gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac gctatcacat
    36421 taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt attcaactcc
    36481 gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc gaacagcttt
    36541 acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaaaaat cagattcagt
    36601 tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag agcgatatcc
    36661 ttaatgcact ggaaaatcat cagtttgcga tttggttaca gccacaggtc gagatgacca
    36721 gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat ggcagttggg
    36781 acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt accgtcggtc
    36841 actgggtgct ggaagagtcc tgtcgattgc ttgctgcctg gcaagagcgc ggcattatgc
    36901 tgcccttgtc ggtaaacctc tcagcgctgc aactgatgca tccaaatatg gtggcggata
    36961 tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg gaagtgacag
    37021 aaagccgacg tattgacgat cctcatgctg cggtggcaat cctccgtccg ctgcgcaatg
    37081 ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg cgtcagctgc
    37141 agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt gttgaaggtt
    37201 tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc ctgaacctac
    37261 aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca aaagcgggcg
    37321 ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc ttcgaagaga
    37381 gttacctgga agaaaaatag ctaccccaaa ctgattacaa aactttaaaa agtgctggtt
    37441 tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca ggtacatttc
    37501 tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccataacctt acaagacctg
    37561 tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt actttcaggt
    37621 cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa taggcgagca
    37681 aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg ctcctgtcat
    37741 cttctgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg tcggtcgtac
    37801 cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga ttattggtct
    37861 tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg caacgcttga
    37921 tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg tcgccttcat
    37981 tatggatgtc atcccggcga gcgtcattgg cgcatttgct agcggtaaca ttctgcaggt
    38041 actgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca aaggccaact
    38101 gatttttaac gtgatcgaaa gtttctcgca ggtcattttc ggcatcatca atatgatcat
    38161 gcgtctggca cctattggtg cgttcggggc aatggcgttt accatcggta aatacggcgt
    38221 cggcacactg gtgcaactgg gacagctgat tatctgtttc tacatcacct gtatcctgtt
    38281 tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct tcaaatttat
    38341 ccgttacatc cgtgaagaac tgctgattgt actggggact tcatcttccg agtcggcgct
    38401 gccgcgtatg ctcgacaaga tggagaaact cggctgccgc aaatcggtgg tggggctggt
    38461 catcccgaca ggctactcgt ttaatcttga tggcacatcg atatacctga caatggcggc
    38521 ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa tcacgctgtt
    38581 aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg gttttatcgt
    38641 gctggcagcg acgctctctg cggtgggcca tttgccggta gcgggtctgg cgctgatcct
    38701 cggtatcgac cgctttatgt cagaagctcg tgcgctgact aacctggtcg gtaacggcgt
    38761 agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac tggacgatgt
    38821 gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat ctcaccactt
    38881 atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa
    38941 cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt catgttcttg
    39001 cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg cagtttttta
    39061 ttaccaggat agttgatcag gggttcacat gcagggcaca aaaattcgac ttttagcggg
    39121 cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc
    39181 agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga ctacacccca
    39241 gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc tcgccgaaag
    39301 tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg
    39361 ccttgacgca gcgcaggcgc gttcattgtg gcagcagggg atcgacccta aacgcccgat
    39421 gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc ccaataaccg
    39481 taacgacttg ctgaaagagg cgctctctta tctggcaaat gccactggca aactgactat
    39541 cacgccagaa accatcaacc atgcgttgca aagtcaggac atggtggcaa cctggcctgc
    39601 cgatactaaa gagggctggt ggcgttatcg tctgaaagga tcaaccttgt taggtcacga
    39661 tcctgccgat ccgctgaaac aacccgttga agcggaaaag attaaagatt tctatcagaa
    39721 atggtacacc ccggatgcaa tgacgctgct ggtggtggga aacgtggatg cgcgctcggt
    39781 cgtcgaccaa atcaataaaa cgtttggcga actgaaaggc aaacgtgaaa caccggctcc
    39841 ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgaccg acgcggtgcg
    39901 tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg aatcagccgc
    39961 actgctgcgc tactggcgtg cggacctggc ccgtgaggcg ctgttctggc atgttcagca
    40021 agcgttaagc gccagtaaca gtaaagacat cggtcttgga tttgactgtc gtgtgctgta
    40081 tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga acagcaacct
    40141 taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt
    40201 caatgcgtta gtggcgcaaa agaaactgga gctgcagaag ctgtttgccg cctatgcgcg
    40261 agctgatacc gatattctga tggggcagcg gatgcgttcg ttgcaaaatc aggttgtcga
    40321 tatcgcgccg gagcaatatc agaaactgcg tcaggatttc cttaacagcc tgacggtgga
    40381 gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa tactgctgca
    40441 gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg atcaaatcat
    40501 ggccccatcg actgcggctg ccgccacatc tgtcgccacg gatgacgtac atcctgaagt
    40561 gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca accatcaggt
    40621 atttatggat gagctggata ggggggtaac actctatcca gccttttgca tatgctgctt
    40681 ttacgctggc atcgcttcac gcgggataat cgcgccgcga tactgaataa cggtacttgc
    40741 ggtcaggtgc ccgcgtttcg ctgcgtcttc cgcactgccg cccgtcaaac gtaccgccag
    40801 ataaccggca ctgaaagagt cgccagctgc ggtggtatcg atcacttttt ctttcggcag
    40861 ttttaccgcc ggaacatcca ctaacccttc accagcaatg gacaccaggc aagaatccgc
    40921 cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc
    40981 caccggctgt tgaccccaca gcgcgtcttc atcgtccagc gtcaggaagg cgatatccgt
    41041 gcattccagc atttgttggt acacctgctg tgtctcttct ttgctggccc acaggcgcgg
    41101 acgatagtta ttgtcgaaaa tcacttttcc gccgttggcg cggcattcgc gcagcaggga
    41161 aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag
    41221 ataatcgaaa ttcgccagct cttcgcaaat cgtcgcggat tgctcactct ccagccagaa
    41281 tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc tgtcggtttc
    41341 aatgtagtaa aggcccggca gacggttttc catccgttgg gtcagggaag tatcaacgtt
    41401 ctcgccgcgc caggcgtcca gcatctgctg gctaaaactg tccgttccca gcgccgttac
    41461 gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg aagtgttcag
    41521 ggtatcgccc ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct caatcatgca
    41581 ttcgccaatc acggcaatct ttttggacat agttgtgaac ctgatctgta aaaaattagc
    41641 gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt ccattatttt
    41701 tttgagccag aacgtattcc tgaaagattc cgttgtggag tggggaaatt taccccggcc
    41761 aggcggtaaa gttctgcctt tgcgcgccga taatctttgt cgagtccggg cagcatcact
    41821 tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag caaccctgaa
    41881 gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc
    41941 tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt aacggtggtc
    42001 acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac tgaaatcacc
    42061 gttagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca aaaagccgac
    42121 ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac gctcatcgcc
    42181 ctgcgtcagc aaccaaaaat cctgcgccag attgaggagc ttccctggct gcgtttcgaa
    42241 ctggtggaac acatccgcct gccgaaagat tcaacctttg cctcgatgtg tgaatttggc
    42301 ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact aagtgaagtg
    42361 cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca gtcaccggaa
    42421 ggacgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg cggggtgatt
    42481 gtcgagggcg tagaaacacc agaagagtgg cgtgatgttc agaactcgcc cgcattcgcc
    42541 gcacaaggct ggtttctttc acgcccggca ccgatagaaa cgctgaatac ggcggttctg
    42601 gcgctataag ctgcctcatt ttccgcctgg ctggactatc tttaggactg gcacagaaag
    42661 aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat ttcaggggct
    42721 ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg gaatcgactc
    42781 aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc tatccgtggc
    42841 gatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg
    42901 tggccccatg tacacgcgga agacatcatt cttggcaatc caccggatat tcccgaagtc
    42961 acaatggtgc atttgccgcg agtagaagcg acgctggccc cgctggcgct gctgaccaaa
    43021 acggtctggt tgccgtggat caagctcgaa aagcccgacg cgcgcctgat tcgcctctcc
    43081 gaaaagaaca ataactggac gtttaatctc gccaacgatg acaacaaaga cgcgaatgca
    43141 aagccgtcgg cgtggtcgtt tcggctggat aatattctgt tcgatcaagg gcggatcgcc
    43201 attgatgaca aagtaagcaa agcggatctg gaaatttttg tcgatccgtt aggcaagccg
    43261 ctgtcgttca gcgaagttac cggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat
    43321 tacgtttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac tggtacggga
    43381 aaaataggcg gtatgctggc gctgcgtggc gaaggaacgc cgtttccggt acaggctgat
    43441 ttccgctctg gtaacacccg tgttgctttt gatggcgttg tgaatgaccc aatgaagatg
    43501 gggggtgtcg atttacggct taaattttct ggcgattcgc tgggtgatct ctatgaactg
    43561 acgggcgttc tgctgcccga tacaccgccg tttgaaacgg atggtcggct ggtagcgaaa
    43621 atcgatactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg cattggcgat
    43681 agcgatatcc acggttctct gatctacacc accggcaagc cacgaccaaa actggaaggt
    43741 gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg cgttgattcc
    43801 gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa aagcgttcag
    43861 cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga cgttatggat
    43921 gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gcagcctgcc gattagcgat
    43981 ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc
    44041 atagcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa gccgatgcag
    44101 gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc cgatgtagaa
    44161 ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactgcgcgg tagcggtaac
    44221 tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat gaatgacggg
    44281 ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta cattgtcggt
    44341 gcgatatttg gtgatgatga ggtgcgggtg aactgcgcgg cggcgaatct ggatattgcc
    44401 aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt gattaacgtt
    44461 accggtacgg caagttttgc ttcggaacag ctggatttga ctattgatcc ggagagtaaa
    44521 gggattcgga ttatcacatt gcgttcgccg ctgtatgtac gtgggacgtt taaaaatccg
    44581 caggctgggg tgaaagccgg gccgttgatt gctcgcggag ccgttgcggc ggcactggca
    44641 acgctggtaa caccggcggc ggcgttactg gcgctgatct caccttccga aggggaggct
    44701 aatcagtgca ggacaatttt gtcgcagatg aagaagtgat gataattgcc ggatgtctga
    44761 catccggcaa ttaaacagat ttagattgtt ggcaaagtgt gctttgttca tgccggatgc
    44821 ggcgtgaacg cctccggctt acaaatcctt gcgaattcaa tatattgtat ggaaattctg
    44881 gcctgataag cgtagcgcat caggcttttc actcttatta caacgactga tgtcgcgtct
    44941 catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga tatagcccca
    45001 ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat atacggcgca acggaagccc
    45061 cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggga
    45121 acagctctgg taacagcgcg cccatcggac cgaaggtcag ccccatcaga cttaacccca
    45181 gcagcagaaa ggcaaaaacc agaatcgggt tgccagaacc gagcaaaggg gtaaaggcga
    45241 atagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg
    45301 catcagccag taatccggcg actggcacca tcacgccaaa accaataact gccatcatca
    45361 gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactaa
    45421 aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg
    45481 tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct
    45541 gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat
    45601 acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca
    45661 taaactgctc gtcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga
    45721 agccaatcgg tgcgcccagc tgcgggaagg agccgtacaa tgcacgtttg cgcggtgggg
    45781 cgttttcagt cgccagcagc gccgcgccgc cccattcacc acctaagccc agaccctgac
    45841 caaatcgagc cagcgccagc agcagcgggg cgaaaatacc aatcgtggca tagcctggca
    45901 gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt
    45961 tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga
    46021 aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga
    46081 agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact
    46141 caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt
    46201 attcttgctc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg
    46261 ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg
    46321 aaatggtcaa aaatcaggca aaaagggaat aatgtttagg ccagtgattt ttcttctatg
    46381 aaatgcttca cataatttta taaatggtgg attaaactgg ttttaggtta aataaaagtt
    46441 accaactgga tttatatgct ggggttatga gttggaatga aattgtttgc tcctgaatag
    46501 atggctaaag aggctctggg aaagagtttc aagaggttat gtgcgcatta tagccggatg
    46561 cgatgctggc gcatcttatc cggcctacgg ggagccaggc cggataaatt cgtacgccgc
    46621 atccggccta ttttaaggct gtgttttccc cggcattcgc gggtcgtctt tatattcggc
    46681 ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat
    46741 cagccccagc acggaaccaa atgctgcgcc agagggtgat ttcatcaacg atggcagggt
    46801 gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcggat
    46861 cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag
    46921 atagttggcg aagatggaaa tcgccacgcc aatcaaccgc catgtcggtt tcagccactc
    46981 aatgctgttc aggtgcaggg cgctaataat catttgctgc gcagaaccgg caaccgaggt
    47041 gatagaaagc gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgcag
    47101 atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgtgactg
    47161 ggcgcgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccactgc
    47221 cagaccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa
    47281 tgtggcggct agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag
    47341 caacatcgga tgggaggcga gcacaaagcc ccctgcggca aacgacacca tcaaaatggg
    47401 gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc
    47461 gttaaagcgt tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttctc
    47521 ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc
    47581 caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg
    47641 cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaagtatagt cggtcagtcc
    47701 aggtactctt tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc
    47761 tcccgacgct gcggataaac cagtgaaagg gacagcggct cggcacggta gccaggcaac
    47821 acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata
    47881 atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg
    47941 ctgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt
    48001 acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc
    48061 ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttaccg
    48121 aggggacggg cgataacgcc atcttccggt aatgcgccag tgcgtatcac gcaatcaaaa
    48181 ccatcatgaa gaatatctac cggacggtca tggctactca gttccagctc aattcccgga
    48241 tgcagataga gaaattccga caagcgcggc agtaacaggc ttttcgcgat tcctgacggg
    48301 atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt
    48361 tcgctgagat tactcaacac atcttttgct cgttgataat aggtcatgcc ttctggcgtg
    48421 agtttgaccc gtcgcgtggt gcgctggaga agctgggtgc caagttgatg ttccagtgcc
    48481 tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa
    48541 ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg
    48601 accgattgtt gttcacaacg aaacagtagc gtgcatatcg cattgtttat tgttttttct
    48661 aacatctaat gccgggtttc acaatgttgt catgatgaat gtggcgattt ctcagcagga
    48721 aaagggagga atagctttat gagaaaggta aattccctta aacagagcgt atttttcagc
    48781 gattcccttt aaagtgaata gaagatgtat tggctatttg tgcttatttt tatctcattg
    48841 ccgtttgtta tttttgttta tggtcgcgga atttcatttt taattgcatg cgtactaact
    48901 accgatataa ggaaatcctc aaaaatgttt taaatagtgc cctcgacatg taatttcagg
    48961 cgtttagctg taactattta tatcggtaac tattttctac gtgtcatctc tgtagaatgg
    49021 ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag
    49081 tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt
    49141 caatatttat tcatggaatg aaaatcagtt tacagcatcg tattccagga gtgagtattc
    49201 agggggccag tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag
    49261 tgatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaactgtgg
    49321 tgcaatttcc tgaggttaag gtgttaatta cggcgacgga ttgcaacaaa cggtggttac
    49381 aggaagttat tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat
    49441 ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgaa
    49501 cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt
    49561 taaccatgtt agcggcgggc gaatcaaata aagagatcgg cagggcgctg aatatcagta
    49621 ccggaacggt aaaagcgcat ctggaatcac tgtatcgccg tctggaagtc aaaaatcgca
    49681 cccaggcggc aatgatgtta aatatctcct cctgaagagg ataattttac agccagcagg
    49741 aagttagcgg ctggctgtaa aaatattatg gttcgccgta caaaccaatt aaacggcgta
    49801 ccacaccgtt agtccagcca aacccatcct gcaacggata ctcgccaccg ccgccttcgc
    49861 gaggaacacc atcggcaata tggtatttct cgatcagttt gtgctgttcc agatagaact
    49921 gattcaccgt cttcagccag ttgcgcgcga tttcatcacc cagaaggtca tcgccgtaca
    49981 tcttaaatcc ctgaattgcc atccactgta acggtgccca gccgttgggt ttatcccact
    50041 gttcaccggt ttcgtactcg cttgccagaa tcccgccagg tgtcagtaac cggctgcgca
    50101 cggcgtttgc cagacgatcg gcctgttcat ggttcgccat accgacatag agcggcacaa
    50161 tggcggcagc ggaaaacagc gccagttgct cgcgtcgcca gtcgtaatcg cggtagatac
    50221 cgttttcatc atcccagagg taacggttta ccgcatcgcg acgggcactg gccttctggc
    50281 ggaacagtgc ttctgtctct ttctcgcctt tcagtgccga gatgttggcg atggcgctct
    50341 ccagtttaaa caggaaggca ttcagatcga tggggatgaa ctgggtggta cgaatgctcg
    50401 ccagacgacc agtatcacgc agccaacggg aagagtaatc ccaaccggag gccgcccccg
    50461 cacgtaaatc gcggtacacc tcgttaggcg ggcgaccaga atgtttcgcg gtttcaacgt
    50521 cctcaagcca ggattcgtca cgcggcgtgt cgcgatcgtc ccagtaacgg ttgagcagcg
    50581 agccgtccgg catccgcaca acatggcgat aggcctgatt agggatcaac gattctgcac
    50641 cgtccatcca gaaggcatat tccattttca ggtggtcgag atagcggcgc gcgccgcgta
    50701 caccatcttc ttcaaacaac tccaccatca gcgcaaaaac cggtggttgc gagcggctca
    50761 aataataggt gcggttgccg ttggggatgt ggccgtagtt ttcgatcatc caggcgaagt
    50821 tatcggccat gcatttcagc aaatcttccc gaccactttc cgccagcccc agcatggtga
    50881 aataggaatc ccaatagtag gtttcgctaa aacggccgcc cgggacaata tatgactgcg
    50941 gcagcgccag cagagaagac cacggaatgt gatcctgtgg ttcacgggtt agcaccggcc
    51001 acagctggtc gatatgctct ttcagggaat tttgcgggtc cgatacatac tcgctggagt
    51061 agacctccgg cagccagaag tggttttcaa caaacttgcg caagtcaaaa tcacgatgac
    51121 ggcgcacttt acggtagcgg attaaaatat ccagcgggtc cattttgggt gcgcagtcgg
    51181 gaaaggtttt actgtcgggg aaaattttcg ccgactgaac atgctcgaac agttcgagat
    51241 agcgatcggc aggcgtcagc gcatcagagg cgggcagccc ttcaatcatc tcgggttccg
    51301 gttctgcctc gatcatctca tccagtttta attcatacgg gtccagctca tagcagagat
    51361 cgacctcgat catcagttcg tctggattag ggttttgaat tttctgattg agcataagga
    51421 tccgacctcc gaaattcgtc gctaattatc gggcttttct ttaagggtag tcattcgctc
    51481 cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat taacctgcca aaaaatataa
    51541 cgcactaata tcaatggaat ttataaaaat acggatttcg tgttgttttt cattttcaga
    51601 acattcaatc ctgctctcgc cgcaatcatg atatgccatt cctgatctgg gtaataggca
    51661 ctggctatct tattgatagt ttatattcat gtaattgatt gattcctatc aaaaagcata
    51721 tctccctgtt ttttatgatg gctttgtctc gcagagacgt ttgcagcatt ctggaaaatt
    51781 ttctgggtca caataactca ctaccgtgag ggaagaataa tgaaaatggt ctcacgtatt
    51841 accgcgatcg gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg
    51901 taccacgata ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat
    51961 aataaggttt taggctttct ccgcgaaaaa ggatgcgatt attgccatac gccttcggca
    52021 gaattacccg cctattatta tattcccggc gcgaaacagt tgatggatta cgacataaag
    52081 cttggatata aatcttttaa cctcgaggcc gtgcgtgcgg ctctgctggc taataaaccc
    52141 gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccgcca
    52201 acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata
    52261 ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag
    52321 catcgcaatg aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg
    52381 gcgttgggtt ttgcgctgta tcacgatccc cgtttatcgg cggatagcac catttcatgc
    52441 gctcattgcc atgcgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt
    52501 ggtggcgcag ttgggccgat taacgcgccg acggtattta actcagtatt taacgttgag
    52561 cagttctggg atggtcgtgc ggcaacattg caggatcagg ctggtggacc gccgttgaac
    52621 ccgattgaaa tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg
    52681 cagcttaaag cgcagttcct cgaagtctat ccgcaaggtt tcagtggcga aaatattact
    52741 gatgccattg ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg
    52801 ttgcgtggag atgagaatgc tctgacggcg caacagaaaa aaggctatca attatttaaa
    52861 gataataaat gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg
    52921 ttggggctga aaaaagactt taactttggt gaaattacgg cggcggatat tggtcgtatg
    52981 aatgttacta aagaagagcg tgataaattg cgtcagaaag tacccggttt acgtaacgtt
    53041 gctttaacgg caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcagtgaaa
    53101 ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta
    53161 gctttcctgc acagtctgaa cggggtttac atgccgtata tgcaggataa acaataatta
    53221 atttgatcgc ccgaacagga gtgtttgggc gatt
//
LOCUS       DAJOTB010000014        50596 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJOTB010000014 DAJOTB010000000
VERSION     DAJOTB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.014"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            214..840
                     /locus_tag="OHN28_000844"
     CDS             214..840
                     /locus_tag="OHN28_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCQ2296284.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            861..2963
                     /locus_tag="OHN28_000845"
     CDS             861..2963
                     /locus_tag="OHN28_000845"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCQ2296285.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2976..3614
                     /locus_tag="OHN28_000846"
     CDS             2976..3614
                     /locus_tag="OHN28_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCQ2296286.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3678..4346
                     /locus_tag="OHN28_000847"
     CDS             3678..4346
                     /locus_tag="OHN28_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2296287.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4343..5128
                     /locus_tag="OHN28_000848"
     CDS             4343..5128
                     /locus_tag="OHN28_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCQ2296288.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5132..5944
                     /locus_tag="OHN28_000849"
     CDS             5132..5944
                     /locus_tag="OHN28_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296289.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5956..7560)
                     /locus_tag="OHN28_000850"
     CDS             complement(5956..7560)
                     /locus_tag="OHN28_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCQ2296290.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7686..7991)
                     /locus_tag="OHN28_000851"
     CDS             complement(7686..7991)
                     /locus_tag="OHN28_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCQ2296291.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(8100..8176)
                     /locus_tag="OHN28_000852"
     tRNA            complement(8100..8176)
                     /locus_tag="OHN28_000852"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8140..8142),aa:Val,seq:gac)
     gene            complement(8181..8257)
                     /locus_tag="OHN28_000853"
     tRNA            complement(8181..8257)
                     /locus_tag="OHN28_000853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8221..8223),aa:Val,seq:gac)
     gene            8565..9821
                     /locus_tag="OHN28_000854"
     CDS             8565..9821
                     /locus_tag="OHN28_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCQ2296292.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9862..11235)
                     /gene="mdtK"
                     /locus_tag="OHN28_000855"
     CDS             complement(9862..11235)
                     /gene="mdtK"
                     /locus_tag="OHN28_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCQ2296293.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11450..12091
                     /gene="ribE"
                     /locus_tag="OHN28_000856"
     CDS             11450..12091
                     /gene="ribE"
                     /locus_tag="OHN28_000856"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCQ2296294.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12131..13279)
                     /gene="cfa"
                     /locus_tag="OHN28_000857"
     CDS             complement(12131..13279)
                     /gene="cfa"
                     /locus_tag="OHN28_000857"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCQ2296295.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13570..14781)
                     /locus_tag="OHN28_000858"
     CDS             complement(13570..14781)
                     /locus_tag="OHN28_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCQ2296296.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14894..15826
                     /locus_tag="OHN28_000859"
     CDS             14894..15826
                     /locus_tag="OHN28_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2296297.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15823..16848)
                     /gene="purR"
                     /locus_tag="OHN28_000860"
     CDS             complement(15823..16848)
                     /gene="purR"
                     /locus_tag="OHN28_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCQ2296298.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17147..17236
                     /gene="ynhF"
                     /locus_tag="OHN28_000861"
     CDS             17147..17236
                     /gene="ynhF"
                     /locus_tag="OHN28_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCQ2296299.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17402..18571
                     /locus_tag="OHN28_000862"
     CDS             17402..18571
                     /locus_tag="OHN28_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296300.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18717..19298)
                     /gene="sodB"
                     /locus_tag="OHN28_000863"
     CDS             complement(18717..19298)
                     /gene="sodB"
                     /locus_tag="OHN28_000863"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCQ2296301.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19426..20253)
                     /gene="mepH"
                     /locus_tag="OHN28_000864"
     CDS             complement(19426..20253)
                     /gene="mepH"
                     /locus_tag="OHN28_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCQ2296302.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20588..20935
                     /gene="grxD"
                     /locus_tag="OHN28_000865"
     CDS             20588..20935
                     /gene="grxD"
                     /locus_tag="OHN28_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCQ2296303.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20986..21078)
                     /locus_tag="OHN28_000866"
     CDS             complement(20986..21078)
                     /locus_tag="OHN28_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCQ2296304.1"
                     /translation="MNDLPVRQAPMFTLLREAGFSSSPQGLDWG"
     gene            complement(21307..21954)
                     /gene="rnt"
                     /locus_tag="OHN28_000867"
     CDS             complement(21307..21954)
                     /gene="rnt"
                     /locus_tag="OHN28_000867"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCQ2296305.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(22057..22464)
                     /gene="gloA"
                     /locus_tag="OHN28_000868"
     CDS             complement(22057..22464)
                     /gene="gloA"
                     /locus_tag="OHN28_000868"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCQ2296306.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22545..23642)
                     /gene="nemA"
                     /locus_tag="OHN28_000869"
     CDS             complement(22545..23642)
                     /gene="nemA"
                     /locus_tag="OHN28_000869"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCQ2296307.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(23679..24278)
                     /gene="nemR"
                     /locus_tag="OHN28_000870"
     CDS             complement(23679..24278)
                     /gene="nemR"
                     /locus_tag="OHN28_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCQ2296308.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24381..24620
                     /locus_tag="OHN28_000871"
     CDS             24381..24620
                     /locus_tag="OHN28_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCQ2296309.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            24669..25565
                     /locus_tag="OHN28_000872"
     CDS             24669..25565
                     /locus_tag="OHN28_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCQ2296310.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25646..26167
                     /gene="sodC"
                     /locus_tag="OHN28_000873"
     CDS             25646..26167
                     /gene="sodC"
                     /locus_tag="OHN28_000873"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCQ2296311.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26168..28177)
                     /locus_tag="OHN28_000874"
     CDS             complement(26168..28177)
                     /locus_tag="OHN28_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2296312.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQILT
                     MNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREILE
                     QLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRLQ
                     SGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAA
                     ISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATM
                     QLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFAI
                     LRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARRW
                     LLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLE
                     ELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28177..29034)
                     /locus_tag="OHN28_000875"
     CDS             complement(28177..29034)
                     /locus_tag="OHN28_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2296313.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(29037..29273)
                     /locus_tag="OHN28_000876"
     CDS             complement(29037..29273)
                     /locus_tag="OHN28_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCQ2296314.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29474..29908
                     /gene="slyA"
                     /locus_tag="OHN28_000877"
     CDS             29474..29908
                     /gene="slyA"
                     /locus_tag="OHN28_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCQ2296315.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29955..30422)
                     /gene="slyB"
                     /locus_tag="OHN28_000878"
     CDS             complement(29955..30422)
                     /gene="slyB"
                     /locus_tag="OHN28_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCQ2296316.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30696..31805
                     /gene="anmK"
                     /locus_tag="OHN28_000879"
     CDS             30696..31805
                     /gene="anmK"
                     /locus_tag="OHN28_000879"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCQ2296317.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31903..32232
                     /gene="mliC"
                     /locus_tag="OHN28_000880"
     CDS             31903..32232
                     /gene="mliC"
                     /locus_tag="OHN28_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCQ2296318.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32291..32947
                     /gene="pdxH"
                     /locus_tag="OHN28_000881"
     CDS             32291..32947
                     /gene="pdxH"
                     /locus_tag="OHN28_000881"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCQ2296319.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33076..34350
                     /gene="tyrS"
                     /locus_tag="OHN28_000882"
     CDS             33076..34350
                     /gene="tyrS"
                     /locus_tag="OHN28_000882"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCQ2296320.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34412..35272
                     /gene="pdxY"
                     /locus_tag="OHN28_000883"
     CDS             34412..35272
                     /gene="pdxY"
                     /locus_tag="OHN28_000883"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HCQ2296321.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35316..35921)
                     /gene="gstA"
                     /locus_tag="OHN28_000884"
     CDS             complement(35316..35921)
                     /gene="gstA"
                     /locus_tag="OHN28_000884"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCQ2296322.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(36027..37529)
                     /gene="dtpA"
                     /locus_tag="OHN28_000885"
     CDS             complement(36027..37529)
                     /gene="dtpA"
                     /locus_tag="OHN28_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCQ2296323.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(38140..38775)
                     /gene="nth"
                     /locus_tag="OHN28_000886"
     CDS             complement(38140..38775)
                     /gene="nth"
                     /locus_tag="OHN28_000886"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCQ2296324.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(38775..39470)
                     /gene="rsxE"
                     /locus_tag="OHN28_000887"
     CDS             complement(38775..39470)
                     /gene="rsxE"
                     /locus_tag="OHN28_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCQ2296325.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(39474..40094)
                     /gene="rsxG"
                     /locus_tag="OHN28_000888"
     CDS             complement(39474..40094)
                     /gene="rsxG"
                     /locus_tag="OHN28_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCQ2296326.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(40098..41156)
                     /gene="rsxD"
                     /locus_tag="OHN28_000889"
     CDS             complement(40098..41156)
                     /gene="rsxD"
                     /locus_tag="OHN28_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCQ2296327.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(41157..43577)
                     /gene="rsxC"
                     /locus_tag="OHN28_000890"
     CDS             complement(41157..43577)
                     /gene="rsxC"
                     /locus_tag="OHN28_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCQ2296328.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANA
                     EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAA
                     QQKVVNED"
     gene            complement(43570..44148)
                     /gene="rsxB"
                     /locus_tag="OHN28_000891"
     CDS             complement(43570..44148)
                     /gene="rsxB"
                     /locus_tag="OHN28_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCQ2296329.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(44148..44729)
                     /gene="rsxA"
                     /locus_tag="OHN28_000892"
     CDS             complement(44148..44729)
                     /gene="rsxA"
                     /locus_tag="OHN28_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCQ2296330.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(44806..45246)
                     /locus_tag="OHN28_000893"
     CDS             complement(44806..45246)
                     /locus_tag="OHN28_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCQ2296331.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(45332..45547)
                     /gene="ydgT"
                     /locus_tag="OHN28_000894"
     CDS             complement(45332..45547)
                     /gene="ydgT"
                     /locus_tag="OHN28_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCQ2296332.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(45819..45944)
                     /gene="blr"
                     /locus_tag="OHN28_000895"
     CDS             complement(45819..45944)
                     /gene="blr"
                     /locus_tag="OHN28_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCQ2296333.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            46187..47227
                     /locus_tag="OHN28_000896"
     CDS             46187..47227
                     /locus_tag="OHN28_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2296334.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(47263..48264)
                     /gene="add"
                     /locus_tag="OHN28_000897"
     CDS             complement(47263..48264)
                     /gene="add"
                     /locus_tag="OHN28_000897"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCQ2296335.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(48368..49540)
                     /gene="malY"
                     /locus_tag="OHN28_000898"
     CDS             complement(48368..49540)
                     /gene="malY"
                     /locus_tag="OHN28_000898"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCQ2296336.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(49550..>50596)
                     /gene="malX"
                     /locus_tag="OHN28_000899"
     CDS             complement(49550..>50596)
                     /gene="malX"
                     /locus_tag="OHN28_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCQ2296337.1"
                     /translation="VPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINSAGDFGPML
                     FGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSCPTTHGFSE
                     SATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVVGGTTEPLE
                     FLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHGLSTKWYMV
                     PVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGYNVPAILEA
                     LGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQVVIGPQVQS
                     VKDEMVGLMRTVQA"
BASE COUNT        12870 a        12395 c        13099 g        12232 t
ORIGIN      
        1 gataacgatc aatgttaaga gcccagaata atcacattat tttttctcct aaccataaag
       61 gattagtttg ttagcgttaa atcttttctc atacactatc gggttaggga aaatatctgg
      121 tgttgaggac agcgtcagca gcgtattagt tgagtgctta atatcaggct gttgaattgt
      181 cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta
      241 aatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggttta
      301 acaattgccc ctgctttact gtcacttttg ggttgcaaac aggaagatat tgatagcggc
      361 acagtagggt tgataaatac acccaaaggg gtgctggtga cccaacgtgc gcgatgcacg
      421 ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc
      481 ttctcccgta tcaaaatcca tcgcaattat ttctttggcg acaacggggt tggctctggc
      541 ggtggcctgt atggcgatct caactatacc gcggatacct gccgtcaatg caaagagtcg
      601 caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt
      661 accgtcgatc ataaacgttg tattggctgt agcgcctgta ctacggcatg cccgtggatg
      721 atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc
      781 gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga
      841 atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc
      901 tcacgacagg aaatattacc ctcgaagatt ccagtaaatt taaaagtttt gtcggtggca
      961 tgggcttcgg ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg
     1021 aagcgaataa attagttttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt
     1081 ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtc gtcgatgccc
     1141 atatgggggg cttttttgca gcgcaaatga aattcgctgg atacgacgtc attattatcg
     1201 aagggaaagc gaaatcaccg gtatggctga agattaaaga tgacaaagtt agccttgaaa
     1261 aagccgattt cttatgggga aaagggacgc gcgcaacgac ggaagaaatt tgtcgcttga
     1321 ccagtccgga aacctgtgtg gcggctattg gtcaggctgg agaaaacctt gttcctctct
     1381 ctggcatgtt gaatagccgt aaccacagcg gcggtgcggg aactggcgca ataatgggtt
     1441 cgaaaaacct gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc
     1501 aggagatgaa gcgtctcaac gattacatga tgactgaact tatcggtgcg aataacaacc
     1561 atgtcgtgcc aagtacgccg caatcgtggg cagagtattc agatcccaag tcacgttgga
     1621 cagcacgtaa agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa acgggggaaa
     1681 ttccgccagg caatcagaat acggtcggct ttcgtaccta taaatccgtt tttgacttag
     1741 gtccggcggc agagaaatac acagtaaaaa tgagtggctg ccactcttgc ccgatccgtt
     1801 gtatgaccca aatgaatatt cctcgggtga aagagtttgg cgtgcccagc acaggtggta
     1861 acacttgtgt agcaaacttt gtccatacca ccatcttccc gaacgggccg aaagattttg
     1921 aagataagga cgatggtcgt gtgattggta acctggttgg tctgaatttg ttcgatgact
     1981 acggcctatg gtgtaactac gggcagttgc atcgcgactt tacttattgt tacagcaaag
     2041 gtgtgttcaa gcgtgttctg cctgctgaag agtatgcgga aattcgctgg gatcaactgg
     2101 aagcgggtga cgttaacttt attaaagatt tttactaccg tctggcgcat cgcgtgggtg
     2161 agttgagtca cctggctgat ggttcatatg ccatcgcaga acgctggaat ttgggtgaag
     2221 agtactgggg ctacgcgaaa aataaactct ggtcgccgtt tggctatccg gttcaccatg
     2281 ccaatgaagc gtcagcgcaa gtcggttcca ttgttaactg tatgttcaac cgtgactgca
     2341 tgacgcatac ccatatcaat tttattggtt ccggcttgcc attgaaactg cagcgtgaag
     2401 tggcgaaaga gctttttggt tctgaagatg cttacgatga aaccaaaaac tacacaccga
     2461 tcaatgacgc aaaaattaaa tatgctaagt ggtcgctgtt gcgggtctgt ttgcataacg
     2521 ccgtcaccct gtgcaactgg gtctggccaa tgaccgtttc gccgctgaaa agccgtaatt
     2581 accggggtga tctggcgctt gaagccaaat tcttcaaagc gatcactggc gaagatatga
     2641 ctcaggaaaa attagattta gctgcagagc gtatctttac gttgcatcgc gcctacacgg
     2701 taaaactgat gcaaaccaaa gatatgcgta atgaacacga tcttatctgt tcctgggtat
     2761 tcgacaagga tccgcagatc ccggtcttta ctgaaggtac agacaaaatg gaccgtgacg
     2821 atatgcatgc ttcgctcacg atgttctaca aagaaatggg ctgggaccca cagcttggtt
     2881 gtccgacccg cgaaacattg cagcgtctgg ggctggaaga tattgccgcc gatctggcag
     2941 cacacaatct actgcctgcg taaggaatgg gagagatgaa ccatcaggat gaattgccgt
     3001 tagcgaaagt cagcgaaatt gatgaggcga agcggcaatg gttgcagggg atgcgtcacc
     3061 ccgttgatac ggtaaccgaa ccagaaccgg ctgaaatcct ggcagagttc attcgccagc
     3121 attctgcggc tgggcagctg gtggcgcgcg ccgtgtttct ctcgccaccc tattcggttg
     3181 cagaagagga actgtcagtt ttgctggaaa acattaagca gaacggagac tacgcggata
     3241 tcgcttgtat gacgggaagc caggatgatt actattactc aacgcaggcc atgagtgaaa
     3301 actacgcggc gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc attgcacacg
     3361 ccgttcgttt tgagtgccag acgtatcctc gtccttacaa agtcgccatg ctgatgcaag
     3421 cgccatatta cttccaggaa gcgcagattg aagccgcgat cgccgcgatg gatgttgcac
     3481 ctgaatatgc cgatatacgg caggtggagt catcgacggc agtgttgtac ttgtttagcg
     3541 agcgatttat gacctatggc aaggcgtatg gcttatgcga atggttcgag gtagagcagt
     3601 ttcagaaccc ctgaaatttc agggggatgt catggatagg gtggacagtt gcagccactg
     3661 cattagggga taatcagatg tccttcactc gacgcaaatt tgttctgggg atgggaacag
     3721 taatcttttt tactggttct gcgtcttctc tgttagcgaa tacgaggcaa gaaaaggaag
     3781 ttcggtatgc catgattcat gacgagtcac ggtgcaatgg ctgtaatatc tgcgcccgcg
     3841 catgccgtaa aactaaccac gtccccgctc agggaagccg tttatcgata gcacacattc
     3901 cagtcatcga taacgacaat ggaacgcagt atcacttttt tcgtcagtca tgtcagcact
     3961 gcgaagatgc tccttgcatc gacgtttgcc cgacaggggc gtcgtggcgt gatgagcagg
     4021 ggatcgtgcg ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt ggcgtttgcc
     4081 cgtaccaggt tcgctacctt aaccctgtca ctaaagtggc agataaatgt gatttctgcg
     4141 ctgagacccg actggcaaaa ggctttccgc ccatttgcgt cagtgcctgc ccggaacacg
     4201 cattgatctt tggtcacgaa gatagcccag aaattcaggc gtggttgcag gaaaataaat
     4261 actatcaata tcagctacct ggcgccggga aaccgcatct gtatcgccgg ttcggtcaac
     4321 atttgattaa aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt ttcagagcca
     4381 gttggcgaac tatgtgccac agttcacccc ccaattttgg ccggtgtggt tgatcattgc
     4441 cggactattg ctggttggga tgtggttggt gctggggcta catgccttgc ttcgtgctcg
     4501 tggcgtgaag aaatcagcca ccgatcatag tgagaggatt tatctttaca gcaaagcggt
     4561 cagattatgg cactggtcga atgcgttact ctttgtattg ttgctggcaa gtgggctgat
     4621 aaatcacttt gcgctggtgg gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt
     4681 ttgcggattt ttgttactgg catgctggct tggctttgtt ctgatcaatg ccgttggggg
     4741 taatggtcac cactatcgca ttcgtcgtca gggttggctg gatcgagcgg caaaacaaac
     4801 gcgattttat ttgtttggca ttatgcaggg ggaagaacat cctttcccgg caacaaccca
     4861 gtctaaattt aatcccttac agcaggtcgc ctatgttggt gtcatgtatg gattgttgcc
     4921 attgttacta ttgacggggc tgctgtgtct ctatccgcaa gccgtgggag atgtgtttcc
     4981 tggcgtaaga tactggttat tgcaggcgca ttttgctctg gcatttataa gcctcttttt
     5041 tatcttcggt catctttatc tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag
     5101 catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag acctgcgaga
     5161 atggcgtatc ggagcggtga tgtatcgctg gtttctgcgc catttcccac gtggcggtag
     5221 ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg cggagagtct
     5281 ggttgagtat gcgtggaaaa aatggctggc agatgaaaac ttcgcgcatc aggaagtttc
     5341 ctctatgcaa aaactggcca cagatcccgg agaacgacta ttttgcagtc agtttgtccg
     5401 cagcgacgat catgctcgcc tcggctgttg tgaggataac gccagaatcg caaccgctgg
     5461 gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg tcggctttaa
     5521 cagtcacatt ggcagctccg gtgcacgcgc acgggttgct gtaaccggta attcttcgcg
     5581 gataagcagc gctggagata gcggtcggat tgcgaatacc ggaatgcgag tacgcgtttg
     5641 tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa tcgccagctt
     5701 tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg ccagtggtga
     5761 aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc cagggggatg
     5821 cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga
     5881 aaacaatatt cgagccggag tccgctatcg tcttaatgaa caacatcagt ttgttgagtg
     5941 ctgatagctt tacctttaat caaacgaaag acgccgacgc gcacgggatg caggttttac
     6001 gaccgcccga gccatcgcct ctccaatttc tgcacatgcc gtaattccct gaacgaaagg
     6061 ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgcccacgaa tatcttcggg
     6121 ttgctgtaac gtataatctt cgccaacatt agggatttca tcccctgttt tctgtaattg
     6181 ttcgcgtagt ccagggaaag gaatatcttt cactttaagc ggacgctgcc cgcgggcatc
     6241 aataaaaacg tcaaacgagt agctattgtc ttccgttttc aggacggtgc gcgactcatt
     6301 aatttccatt tcgtagtctt caccgagggc gagaatatga atgattcccg cttcacgtaa
     6361 ggccagtagt cgacgaatag actctgaagg gattgccgca taattatcga tgaaaacccg
     6421 ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgtggaa caatttcctg
     6481 tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt tttctcgttt
     6541 attgcgttcg acttcctgga gattttcttc tgcccagtca aatgggtcgc gttgtttgcg
     6601 ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg ctatgcgttg
     6661 gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata cccgatccaa
     6721 aaggcccact tcgccttttt gaatctcagc atttaatgcc tgatcggtga tgatgtgtaa
     6781 gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacctg tgcgcgacat
     6841 taacgtaata tttagctttt catttgcgtt atcgcggtga aaagtgacgt gttgtttatc
     6901 atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca gtccgctcaa
     6961 ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca ggcctgacca
     7021 cgggctggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt gccccgttgc
     7081 gatcaccgct aaatcaaacg tctcgctggg taaattcaga tttgtagcga gcatgacgcc
     7141 agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca ctgcaaattt
     7201 ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag
     7261 aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat aacgctggag
     7321 atggctggct tcttgctttt gtagccattc gagatacgtg caataaatcg gcggtatttc
     7381 aatactggca atgtttgcca gcatcagttt tgagttttcc tcatcactgt atggcatccc
     7441 gataccggct tcgtcagcct gatcgaagat agaaatagaa agtggcgttt gttgctgtag
     7501 aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa ttgttttcat
     7561 tgttacttac ctgtacgcat tctttcgaat ttagtgttat caggtaagtt tggttgagat
     7621 ttttagaatt tgagaaattt cagacaggga tgaaaagggc actaaccgcc cttgattaaa
     7681 atctgtcagg caagttttgc ctgattgatt tgactcagtg gcacattgac atcgaaaact
     7741 ttggcgacaa cttcctcaac gccgagattt ttcagtcggg cagtatgttt ttccagatag
     7801 gcaagggcgc ttttttcatc agtgaaaagg tagatcccac cggcttcgtg gttcttttca
     7861 ctttctgtcc aaaccttcca cagaaaacca ggttcctgat taatcgactc agcaagcggt
     7921 ttaagctgct cagccattgt gtcgccaaat gggccattga aagaaaaatg aagttgtaac
     7981 aaagttgcca tagcgattct ccttctgata acgcatacga agacagtgta aatcttttaa
     8041 cgtattggat acagagaaga aacagaggga aaaaaggaga agaccagatt aagaaaatct
     8101 ggtgcgtccg agtggactcg aaccaccgac ccccaccatg tcaaggtggt gctctaacca
     8161 actgagctac ggacgcagga tggtgcgttc aattggactc gaaccaacga cccccaccat
     8221 gtcaaggtgg tgctctaacc aactgagcta tgaacgcaac gttgtaggtg acaacgggga
     8281 cgaatattag cggcagagtg ggaaggtggc aagaggcaaa acgtaatttt ctgcgctatt
     8341 tcgaccgttt gcagagcttt taagcaaatt ggctatattt tgttgatttg caagggcgat
     8401 ttttattcag gatcgcattt acatctgata caacccaata atccgtgtat cagagtttaa
     8461 acagagttgt ttgccaccat ctcaaatctg tcgtggataa atccttcgta gcctttacac
     8521 ttaggtagca ataaaggcac acagatgcag caaaggaata aatcatggga tctgatgcga
     8581 aaaacttgat gagcgacggg aacgtgcaaa ttgttaagac cggcgaggtt attggcgcga
     8641 cgcaacttac tgaaggcgag ttgattgttg aagctggcgg aagagccgaa aataccgtgg
     8701 tcacgggggc tggctggttg aaagtggcaa ccggtgggat agccaaatgc acacagtacg
     8761 gtaacaatgg cacactatcg gtcagcgacg gtgccattgc cacagatatt gttcagtccg
     8821 agggaggcgc aattagtctc tctacgctcg ctacggttaa tggccgccat cccgaaggtg
     8881 aattcagcgt tgataaaggt tatgcctgcg gtttgttgct ggaaaatggc ggtaacctgc
     8941 gtgtactgga aggccatcgc gcggaaaaaa ttattctcga tcaagagggt ggcctgttgg
     9001 tcaatgggac aacctcagcg gtcgtggtag atgaaggtgg tgaattgttg gtgtatccag
     9061 gtggggaagc ctgcaattgt gagattaatc agggcggcgt ttttatgctg gcggggaaag
     9121 ccagtgatac gttgcttgct ggtggcacca tgaataatct cggtggtgaa gactctgaca
     9181 ctattgttga gaatggagcc atctatcgtc tggggacgga tggtcttcag ctctacagtt
     9241 ccggtaagac gcaaaacctg tccgttaatg tgggtggtcg ggctgaagtg catgccggta
     9301 cgctggaaaa tgcggtaata caaggtggga cagtgatcct gttgtcaccc accagcgcgg
     9361 acgaaaattt tgtcgtagag gaagatcgcg caccggttga actgaccggt agtgttgcat
     9421 tactggacgg cgcttcaatg attattggct atggcgcaga tctgcaacaa tcaacgatta
     9481 ctgtacagca gggcggtgta ttgattctcg acggcagtac ggtaaaaggt gacagtgtca
     9541 cttttggtat tggtaacatc aatctaaatg gtggaaaact gtggctgatc actgatgcgg
     9601 caacgcatgt gcaactgaaa gtgaaacgcc tgcgcggaga gggagccatt tgcctgcaaa
     9661 ccagtgcgaa agaaatttca cctgacttca tcaatgtgaa aggggaagtt actggtgata
     9721 tacacgttga gataacagat gccagtcggc aaactctgtg taacgcactg aaactacagc
     9781 cagacgaaga cgggattggc gcaacgctcc agcctgcgta aaaacacagg cgcaacccgt
     9841 gaaggttgcg ccttgtcttt actagcgagc tgctcgttgc agaatgatgg ctgacggcag
     9901 acgttgcagg aaccgcatac gcagcatcat cataatggct gccgaagtca ggccaataat
     9961 aaagcctatc cagaagcctg ctggccccat aggttcaacg accagatcgg tcagtgccag
    10021 aatatagccg cttggcaagc ccagcaccca gtaagccgta aaggtgatat agaaaatgga
    10081 acgtgtatct ttataaccac gcaaaatccc actgccaatc acctggatag agtcagaaat
    10141 ctgatatacc gccgccagca gcatcaaatg cgcagccagc gttacaacct cgggattgtc
    10201 gttgtacaac agggcgattt gctctcgcag tgaaaccgtg aatatggccg tcagggttgc
    10261 catacagaca cccaccataa gcccggtccg cgcagcggtt tgcgcatcca gcgttgagcc
    10321 ttgacccaga cgataaccta cgcggatggt taccgctgcc gccagcgaca tgggaagcac
    10381 gaacatcagt gaactaaagt tcagggcaat ctgatgtcct gcgacatcaa caataccgag
    10441 cggagacact aacagagcca cgacggcaaa cagcgttact tcaaagaaca gtgccagcgc
    10501 aattggcaaa ccgagttgaa tcagtcgttt cataaccgca ggatcgggct tcgcggtgcc
    10561 tttttcgtta cgaatatcgc gcatcgagcg ggcgcgttta atgtaagaaa ccatggcaag
    10621 gaacatgacc caatacaccg ccgcagtagc cacgccacaa ccaacgccac cgagctcagg
    10681 cataccgaaa tgaccataaa taaagatata gttcaccgga atgttcacca gcaggccgat
    10741 aaagcccatc accatacccg gcttggtttt tgccagacct tcacactggt tacgggcaac
    10801 ctggaagaaa agatagcccg gcgcgcccca tagcaacgca cgcagataac ccacggcttt
    10861 gtccgccaga gccggatcga tgttttccat ggagcggata atgtaacccg cattccacag
    10921 taccagcata atgagaacgg aaacaaaacc tgccagccag aaaccttgtc gcacctgatg
    10981 cgcaatgcgc tcacgtcgac cggaaccatt taattgcgcg ataaccggcg ttaatgccag
    11041 cagcagtccg tgaccaaaga ggatcgccgg aagccagata gaagttccga tagcaaccgc
    11101 cgccatgtcg gtggcactat agccgcccgc catcacggta tcgacaaaac ccatcgcagt
    11161 ttgggcgatt tgcgcgagaa tcaccgggat tgctaatgct aataacagac gcgcttcact
    11221 gatatacttc tgcacgtgaa caccttttat ttgtagttat atgagagact aaaaagccgc
    11281 ctgaatgggc agcaaaaaga agaagcaggg gaaattcagt ctattgtagc gaggatttat
    11341 taattctcca gtgaaaaaat agccattctt accccttcac tggtaagggc gttttccacc
    11401 tgctattgtg ctgaacagaa tgtcttaact gatttcagga gttgtaagta tgtttacggg
    11461 gattgtacag ggcaccgcaa aactggtgtc gattgacgag aaaccaaatt ttcgcacgca
    11521 tgtggtggag ttacccgacc acatgctgga cggactggaa accggtgcgt ccgtggcgca
    11581 taacggttgc tgcctgaccg tgacggaaat taacggcaac catgtcagtt ttgacctgat
    11641 gaaagaaacg ttacgcatta ctaatcttgg tgatttaaaa gtgggggatt gggtaaacgt
    11701 tgagcgtgcg gcgaaattca gcgatgaaat tggcggacac ttaatgtcag gtcatattat
    11761 gaccaccgct gaagtggcga aaatattaac ctcggaaaat aatcgtcaga tctggtttaa
    11821 agtccaggat agtcagttga tgaaatatat cctgtacaaa ggatttattg gcatcgacgg
    11881 tattagcctg accgtcggcg aagtcacgcc aacgcgtttt tgcgtccatt taattccgga
    11941 aacactggaa cgcacgactc ttgggaagaa aaaactcggc gcacgcgtca acattgaaat
    12001 cgatccacaa actcaggcag tggtagatac ggtagaacgt gtgctggcgg cacgagaaaa
    12061 tgccatgaat caaccaggta cagaagcctg atctgaaatc gcccccggag cgggggcgat
    12121 agaatagcct ttagcgagcg actcgaaggc cgttttcaac accgcgtgag aacacgacct
    12181 gccagagctg aatatcacgg gcgcggaaag cacctgcaca ggcattcaga taataggtaa
    12241 acatccgttt aaagcgttca ctgtagttat ccgcaatttc tggccatgcg gcgaggaatc
    12301 gttcatacca cgccatcaac gtagtatcgt aatcagcacc gaagttatgc cagtcttcca
    12361 tcacaaagtg gggttcgctg gactgagcta tctggcgtac agagggcagg caaccgttcg
    12421 gaaaaatata tttattaatc cagggatcaa cattcagatc ggtttttttc gaaccgatgg
    12481 tatggagcag gaatatacct tccggtttca aattacgatc caccaccgca aaataggtat
    12541 cgtaattttt cggtccgacg tgttcgaaca tccccacaga aacaatacga tcaaactggt
    12601 cgttcaggtc acgataatct tgcagcaaaa tggtgacatc caggccttca cagcgttcct
    12661 gagccatttt ttgctgttcg gcagaaatgg tgacgccgac cacgcttacg tcataattag
    12721 acgccatgta gtgcgccagt ccgccccagc cacagccaat atccagtacg cgcatccctg
    12781 gttttaactg caatttttca caaatcattt tgagcttcgc ctgctgggca gattccagat
    12841 tatcggcatc tttccagtaa gcacaggaat attgcatgaa ggggtcaagc atgcggctga
    12901 acaagtcatt acccaaatcg taatgctctt tgccgactat ccaggcacgt tttttactct
    12961 gcagattgaa gagacgagcg ccggcaatac gcagcgtgtc tttgaaatga tggggaagtt
    13021 ggttctcgag acctgcgcgt aagactttgc taaaaaacat atccagtcgg tcacattccc
    13081 accagccatc catataactt tcgcctaacc ccaacgatcc ttcctgcaga acgcgtttaa
    13141 aaaaatcggg gtttttgaca cgaatatccg ccggggcaga accgttaatg gctataccgg
    13201 cacggctaag taattcgttg gcgatacggt accagttgtc atctggtaca ctgacttctt
    13261 ctatacacga tgaactcata gtttctccat cactggtcgg tgatcagaac cttaaaacag
    13321 cgtagacgct ttttgggctt tgtgagaaat ctcgcagaaa aaaccgcgag tctataatcc
    13381 gacgtagaac agagggaaag gaggaaccct tgccgaaatg accatccata gtgagtcggg
    13441 aacgatcctg tccccgtaat aacataatat ttatcgtagt tttaacaact cgaaattagt
    13501 ataggccgct aatttcaatc atacaacaaa attttgttag cagcctaagt ataagtatat
    13561 cgatatagat cagtgtgatt cactatgagc gacttcggca ttgccatgat tctggcagcc
    13621 aacttcttca caacgttgca tcatgtaacc cagagcgacc agcactactg ttgataacat
    13681 cacgctggtg gtggtgagca atggcgtact gatactgata agccaggaaa ctaccagact
    13741 tgcgaggaag cacagaccca gttgcagagt gttctgcaac gctgcggcgc gaccggttgc
    13801 atgtgggaag ggacgcagcg cctgggcaac aacaatcggg tagatcgcgc cgttggcaat
    13861 cgccatcaca cagaatggga tcaggatttc gaccaacgac acatggctaa tgaagcccgc
    13921 agcccaggtc gcaatgacgc tgacagcaaa cagtaccagc aaccacggta ataactgctt
    13981 gccttgccat ttctgcagcg cggcgcgaca gccataacca ccaatcagaa acgcgatagt
    14041 ttgcgggaca taacttaaac caataactgc cgggctgtaa cccatttcac taaggatgaa
    14101 cggtgaaccg gtcagccatg caaaaaaact ggctgaacag gctgcgtata tcagcacgtt
    14161 gccgcgatag gttttagaac gtagcaggtc ggtaaaggtc agaccatcct gactattgtt
    14221 acgggccttc gtcgtgggtt tgagccagaa aataggcaga atcagcacca cggtaatggc
    14281 aaacagggtg gcgaaaatcg cctgccagga aaaatggacc agcagccagc ttcctaacag
    14341 aggagccagt gccggagata gacccaccag cggcatgatg gtcgcaaaaa tacggttaac
    14401 tttctgtgaa ggataataat ctgtcactaa tgcttgccag ataaccgccg cggcgcagac
    14461 acccacagcc tgtacaaaac gcaatatcag cagcgtggcg gcgttttcca cccacagcat
    14521 ccccagacta cctaacgcaa aaattgtcag gccgatgaat aacaccggtt tgcgaccata
    14581 acggtcggag agtggccccc acagaagctg ggctgcggca aaaccggcaa ggaacagact
    14641 aaggctggca ctgacagcag acgcaggcgt ttgcaggtcg gcctgtatgg cggcgaaagc
    14701 aggcagatac atatcggttg ccagaaaacc gagtacgctc aaacccgcca gccagactaa
    14761 aaatcttttc ccaggttgca tgtatattct ctaaatctct caattaaaca ccgccgcaaa
    14821 gtgtagggag tgaaattagt gttgtgaaac ggtaatattt gatgattggt ttcaaaaatt
    14881 ttgcaggcag actatgtggt cagaatattc tctcgaagtt gttgatgcgg tagcgcgtaa
    14941 tggtagtttt agcgccgcag cccaggagtt gcatcgcgtt ccttctgcgg tcagctatac
    15001 cgtgcgtcag ctggaagagt ggctggcggt gccgctgttt gaacgacgtc accgtgatgt
    15061 ggaactgacc gcagccgggg cgtggtttct caaagaaggg cgctctgttg tcaaaaaaat
    15121 gcagatcacc cgccagcaat gtcagcagat agcgaacggc tggcgcggtc agttagctat
    15181 cgcagtggat aatattgtca ggccagagag aacacggcag atgatcgttg atttttatcg
    15241 ccattttgat gatgtcgaac tccttgtctt tcaggaagtg ttcaacggtg tctgggatgc
    15301 actttccgac gggcgcgtgg aactggcgat tggcgcaaca cgcgcgattc cggtaggcgg
    15361 tcgttatgcc ttccgggata tggggatgtt aagctggagt tgcgttgttg ccagccacca
    15421 cccgctggcg ttgatggatg gcccgttcag cgatgatacg ttgcgcaact ggccgtcatt
    15481 ggtgcgcgaa gacacctcgc gaacgctacc taaacgtatt acctggttgc tggataacca
    15541 aaaaagactt gtcgtgcctg actgggaatc atcggcaacc tgtatctcgg caggattatg
    15601 catagggatg gtgccaacac atttcgccaa accgtggctt aatgagggga agtgggtagc
    15661 actaaaactg gaaaacccct tcccggattc ggcatgttgt ctgacatggc agcaaaatga
    15721 tatgtcgcct gcgttaacct ggttgcttga gtatctcggc gatagtgaga cgctgaataa
    15781 ggagtggctg cgggagccgg aagagactcc cgcaacgggt gattaacgac gatagtcgcg
    15841 gaacgggccg tcagccacgg agcggcgttc aatcaaccgc ggatgcactt caatggactg
    15901 cggttcttca cgtttgttga cgatacgatc caacagcatg ttgaacgctg tttcgcccag
    15961 cgaatctttt ggctgatgga tcgtggtcag cgccggcgta aaatagcggg cgttgcgcac
    16021 gttatcataa ccgatcagcg aaacatcctg cggcacgcgc agacccattt catcagcagc
    16081 acaaagtgcg cccattgcca tgatatcgcc accacagaag acggcagtag ggcgatgcga
    16141 ctgcgacagg atttgctgca tggcgcgata accggattca ggttcaaagt caccctgcac
    16201 aatccagctt tccggcactt tgatcatcgc ttcttccatc gccttcataa aaccagcaag
    16261 acggcctgcg ccggtgttac gttccagcgg gccagggata acgccgattt cgcgatgacc
    16321 gcgttcaatc agataacgcc cggccatgta gccgccttcg aacgcgttat caatgaccgc
    16381 atcggtgaag tcagcttttg cttcacccca gtccatcacc accattggga tatggcgata
    16441 ctcttccagc atcgccagca acggctctgg gtactcagaa cacatcacca gcagaccatc
    16501 gacgcgtttt tgcgccatca tcgacagata agcccgctgt ttctcaagat tgttccacgc
    16561 attgcccaga atcagggtgt aacctttctg gaagcaattt ttttcgaccg cttcaatgat
    16621 ctcggcaaaa taggccgctt cgctgctggt cgccagcaaa ccgatagact tggtgtggtt
    16681 aaccttcagg ctacgcgcca ccgcgctagg ggagtagtgt aattctttga ttgctgccca
    16741 cacggcattg cgcgtttctt cagcgacgaa acgtgttttg ttgatcacgt gtgacacagt
    16801 tgtagtggaa acgtttgctc gtttcgctac atcttttatt gttgccattc catttcactc
    16861 cagaccctaa cttcagctcc tgcaaattcg caaggtaaac gtttgccttt acacacctta
    16921 cgcacaacaa aaatgttgcg gtacgccgga aaaacgacac ggagcgtcag gaaggggtca
    16981 atggccagta cgcaaatagt gtaagcgtgg aattttggcc gatcttgacg aaaaggggaa
    17041 gtagtaaaaa cacttatcat tataaatgac gggagatttt ttcatcacag tgtgtaaaaa
    17101 tgcgtaatat cactttttgt gtggtaataa aaaaggagaa aacttgatga gcaccgacct
    17161 taaattttca ctggtaacaa cgattatcgt cctcggtttg atcgtggccg tgggtttgac
    17221 tgccgcgctg cactgatttc ctctcgaggg agcgcgttct ccctcgcatc tccttcctgt
    17281 ttgttagcaa aatggcaaaa gtgttttctc tttttgttat tttcattttt ttgtgattac
    17341 tgtcatgctt tcgccacttt aatgaatatc gcgtaatgac gcgaagaggt ggagataagg
    17401 catgaaaatt aactatccgt tgctggcgct ggcgattggc gcgtttggta tcgggacaac
    17461 ggagttctcg ccaatgggct tgttgcccgt cattgcgcgc ggtgtggatg tctcgattcc
    17521 cgctgccgga atgttaatca gtgcttatgc agttggcgta atggttggcg cgccgctgat
    17581 gacgcttcta ctttctcatc gtgcccgccg cagtgcgttg attttcctga tggcgatttt
    17641 cacgcttggc aacgtacttt ccgccatcgc gccggattat atgaccctga tgctttcacg
    17701 cattttgacc agcctgaatc acggggcatt ttttggtttg ggttcagtcg tggccgcaag
    17761 cgtagtgcca aaacataaac aggccagcgc agttgccact atgtttatgg ggttaaccct
    17821 ggcaaatatc ggtggcgtgc cggcggcgac ctggttgggc gaaaccatcg gctggcggat
    17881 gtcatttctg gcaacagcgg ggctgggcgt gatttcaatg gtaagtctgt tcctctcatt
    17941 acctaaaggt ggcgcagggg cgcgacctga agtgaaaaaa gagctggcgg tattaatgcg
    18001 tccgcaggtg ctgtctgcat tgctgacgac ggtactgggc gctggtgcaa tgtttaccct
    18061 ctacacctat atctctccgg tactgcaaag cattacccac gtaacgccgg tgttcgtcac
    18121 ggcaatgctg gtgctgattg gtgtcggatt ctctatcggt aactatctcg gcggcaaact
    18181 ggcagatcgt tcagttaacg gcacgttgaa aggctttttg ttgttgctga tggtgattat
    18241 gctggcaatc ccgttcctgg cccgcaatga gttcggcgca gctattagca tggtggtgtg
    18301 gggagctgca acctttgcga tcgtaccgcc gttacagatg cgcgtgatgc gtgtcgccag
    18361 tgaagcgcca ggtctgtctt catcagtcaa tattggtgcc tttaatcttg gaaatgcgct
    18421 gggagcagct gctggtggtg cggtaatttc cgctgggctg ggatacagtt ttgtgccggt
    18481 gatgggggcg attgtcgcgg gactggcatt attgctggtg tttatgtcag ccagaaaaca
    18541 acctgaagca gtttgcgttg ccaacagcta attaaacaaa aacgcagaag gataaccttc
    18601 tgcgttacag ttgctggcaa cgtgcgattt atcttgccga atgcgctgtt aacgcctcat
    18661 ccgcgttggc atggtaagcg aagcgcatca ggtaatgttg catttgccat cagttattat
    18721 gcagcgagat ttttcgctac gaattcccag ttcaccagcg cccagaagtg ctccagatag
    18781 ccagggcgtg cattgcgata gtcgatgtaa taagcgtgtt cccagacatc aacggtcagc
    18841 agcggagtcg catcggtggt cagtggagta cccgcgttag aggttgaaac gatagccagt
    18901 ttgccatcgc tgtttttcac cagccaggtc cagccagaac caaagttttt gatcgctgca
    18961 tcagtaaact gagctttgaa atcggcaaag ctgccaaaag atgcggcgat agcttcagcg
    19021 acttttccag tcggttcgcc acccgcgttc ggtgccaggc agttccagta gaaagtatgg
    19081 ttccagacct gagctgcgtt gttgaatacg ccaccttcag agctgcgaat aatctcttcc
    19141 agtggtttac cttcaaacgc ggtacctttg atcaggttgt tcaggttagt gacataagtc
    19201 tgatgatgtt tgccgtagtg atactcgatg gtttccgcag aaatgtgcgg tgccagagca
    19261 tctttagcat atggtagtgc aggtaattcg aatgacattg ctactctcct ttattattaa
    19321 tttgcatacg tacaatagcc ttattgtgcg tatggatagg gtagcaaatt aaaaggtgag
    19381 acaaaagatg aacttaccct gttgccgtaa caacagggta aagttttagc gaagtgtttt
    19441 tggggtcatt acccggcgag cgccaacata gtggcgctgc cagtagtctt cactgagaga
    19501 agtgatttga atttcctggc ccgtgcgcgg tgactgaata aatttgccgt tgccgacata
    19561 cacgccgaca tgatcggctg taccgcgtcc ctgagtacgg aagaagacca ggtcgccgtt
    19621 tttcagttca ctacgttcga ttggcgctgc atcgcgcagg tgatacattt cattcgccgt
    19681 acgcggaata cgaattttca ccaaatcttt ataagcgtaa taaaccagac cgctgcaatc
    19741 aaaaccggta cgcggtgagc tgccacccca acgatatggc ttaccaattt gctgcatcag
    19801 tttattcatt gccacttttg tggctttttg caccttcgct ttgtgcgcgt cggcaagggt
    19861 tcctgacgcg gcatttttga ctttcacaca atgcgattta tagcccttac gtttggtaca
    19921 cttttcggta aagctggctg aggccgtttt attgacggcg tgtttgctgc gagttttggc
    19981 gtttttagaa gcggtaatgg aagatttttt aacggaagaa gcggttttgc ttttggtcgt
    20041 acttttactg gcggcttttt tcgctgtttt ctggctggct ttctgggtct tttgggtggt
    20101 tgccgtgctt ttctttttat ctgctttttt ggtgatatga gtggtagcag aactctccct
    20161 ggcttgcttt gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca
    20221 gagcgtgatc gaaatacggt ttatccgcgc cactgagcgt ccctctgtta aggcaggtct
    20281 atcatgcctg cgtctgattt acaaaacttg atgattctaa tgcatagaag aagagaaagt
    20341 taatagcttt tattgttaaa aaatacgttt cgtcggcgag cgcaaaaaaa tgcagcagtg
    20401 aaagctaaaa atggttataa atacgccagt ttgccgtgca tcagggaaga aacgaatatt
    20461 tgaaatgcaa caaatagagc aaagggataa aatagcaaaa gcgtgatgaa aatgttattt
    20521 tccgatgccg cactgacccg ctacaatgtc agattactgc cgtaaagaag tttgaggaag
    20581 caagacaatg agcaccacta tcgaaaaaat ccaacgccag attgctgaaa acccgatcct
    20641 gctgtacatg aaaggttcac cgaaactgcc gagctgcggt ttctctgccc aggcagtcca
    20701 ggcgcttgcc gcatgtggcg aacgttttgc ctatgttgat attctgcaga atccggacat
    20761 tcgtgcggaa ctgccgaaat atgcgaactg gccgaccttc ccgcaactgt gggttgacgg
    20821 cgagctggtg ggcggttgtg atatcgtgat cgaaatgtat cagcgtggtg aactgcagca
    20881 gctgatcaaa gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg aataatccgt
    20941 cctgcaatac tgacggatgc gaacgggcat ccgtcagtcc tttctctatc cccaatccag
    21001 cccttgtggc gaacttgaaa atcccgcctc acgtaacagc gtaaacatcg gcgcttgccg
    21061 gaccggtagg tcattcactt ctgttagcgt aaagcgcagc cgtggttcgc gactagacag
    21121 cggtcggctg tttaaaggcg cgaagaaacc agtcgcgggt cgccggcgaa aacacgtccg
    21181 gcaggggcga tgaagggttt ggattatttg ccattaacgc tattccccgt gaatatcatg
    21241 catgatgcgt ggaatagcct cactatagat gcctgaatca ttgcatgtca tcaggcatcg
    21301 actcgattac acctctgcgg cggcaggtag tggccagcct cccagacgtt tccagcggtt
    21361 gacgatttca caaaacagca cagcagtgcg ttcggtgtcg tacagcgcgg agtgcgcctg
    21421 ggtgctgtcg aagtccatgc cagctgtctg gcaagccttt gacaatacgg tttgtccgag
    21481 tgccagcccg gccagtgcag cagtgtcaaa agtggcgaaa gggtggaacg ggttacgttt
    21541 cagtgaggcg cgttctgcgg cggccatcat aaagctgtga tcgaaattgg cgttgtgcgc
    21601 caccataatg gcgcggttac agccgctcgc tttaatacct ttacgtacaa ctttaaaaat
    21661 ttcgtgcagc gcctcgtact cgctgaccgc gccgcgatcg ggatcgttcg gatcaatgcc
    21721 attgaaggca agggcctctg gttgcaaatt tgcgccgaca aatggttcga cgtggaaatg
    21781 taatgtggtg tccggcatca gccagccttg ttcatccatt ttcagggtga tggcggcaat
    21841 ctcaagcagc gcatcggttt tggcgttaaa tccggctgtt tcaacatcga tcacaacagg
    21901 ataaaaacca cgaaaacggt cgcacagacc ggtaagttga gcgttatcgg acatctgatt
    21961 ctcttaatac ggataaattg cagcgcgcat tatgacaaat attacttgtc gatgcagtaa
    22021 agatgcgggc gcgatgagtt cacgcccggc aggagattag ttgcccagac cgcgaccggc
    22081 gtctttctct tcgattaatt caattttgta accgtccgga tcttccacaa atgcgataac
    22141 cgtagtaccg ccttttaccg gacccgcttc acgggttacg ttacccccgt tttgacggat
    22201 tttttcgcac gcttcagcgg cgttatctac gctaagcgcg atgtgaccat aagcagtgcc
    22261 gagttcgtat ttatccacgc cccagttgta ggtcagttca atcactgctt cttcggtttc
    22321 cgggccgtaa ccaacaaacg ccagtgagta tttgtattcc gggttttcgc tggtacgcag
    22381 cagtttcatg cccagcacgt tggtataaaa atcgatggag cgttgcagat cgccaacgcg
    22441 cagcatggta tgaagaagac gcatctttat atcctcaaaa tggctaacag attcaaaaag
    22501 ttgttttagt atagcggcgg ctttgcgccg ctcgcaatgt tggattacag cgtcgggtaa
    22561 tcagtgtaac cttcagcacc gccaccataa aaactttcgg cacgctgcgg gttaagctca
    22621 gctttgcgct gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg accaaatgct
    22681 accgcatcaa ttaacccttt gccgatcagc gtttcagctt tttctactgt gtatgcacct
    22741 gcgccgataa tcggaccgtg gaaacgggcg cgtacttttt cgcgaaacgc atcagtatat
    22801 ggttcacctc ccgcccaatc tggttctgac atatgaagat aagcaatgcc gcgtttaccc
    22861 agttgttcaa tcagatacag tgcatcggct tcttcattcg ggccgttatc cgtgttctgg
    22921 aaagtaccga ttggcgaaat gcgaatgcca atgcgatcgg caccccattc ttcaatcccg
    22981 gcatcgacca cttccagcac cagacgcgcg cgattttcca cgctgccgcc gtactgatcg
    23041 gtacgatggt ttgaagaagg ggaaaggaac tgatgcagca aataaccgtg agcagagtgg
    23101 agctctacca gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg gaaatcattg
    23161 acgatacccg gaatctcttc cagttcaagc gcacgcggca tggatgtttc aacacggatc
    23221 gcctgaccat tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc tgaaggcgct
    23281 accggtgcct gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt gtgccacagc
    23341 tgtacggcca tatgaccatt ttcagcatga acgccagcgg taattttttt ccatgcggca
    23401 atttgttccg ggctatggat gccaggtgca cctgcgtatc cttttgcctg ggcagaaatt
    23461 tgcgtggctt cgctaataat caaaccggca ctggcacgtt ggcgatagta ttccgccatc
    23521 aacggggtag ggatgtcacc cggttcaata ctgcgcagac gcgtcagcgg tgccataaaa
    23581 atacggtttg ccgccgtgat cgcgcccact ttcagtgggg aatacagttt ttcagatgac
    23641 ataaaggctc ccggtcgtct ggtgataaaa aatgcctgct aaacggcagg cgtcgcaata
    23701 atgtttttta catgggccag cgcgttttcc agtggctcga aactgcgcga aattttggcc
    23761 tgcaaattcg caccaagcca cagtgcgtaa agcacttgtg cctgttgcag cggttcgcca
    23821 caaaaggtta aacaatggtt ctcacggcca ttttccagcg cctgagagag cagggcgatc
    23881 acgccgcgag cccctttatc cattgcgctg cgcatatctt ctgacagatc gcacacttcg
    23941 gcagagagtt ttactgtcag gcaaccactg atggttccat gttggcaaaa ctggttcagt
    24001 gtttgctggt aataagccag tatgcggtcg cggtagttac cttcgccgga ttgcagcaac
    24061 tcagtcagtc gctggtgata tgcggcgtaa tggcgctcaa gcatggcaac gccaaacgct
    24121 tctttagaac gaaagtagtg atagaaggac cctttcggca cttcagcggt ttttaggagt
    24181 tcgcttagcc ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc cgtcgccagg
    24241 agatgttcgc gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt agtagaccag
    24301 tcggtctagt gcaagcaaag ttgctacttt ggacattaac agcttcaata atggtaataa
    24361 tcttatctga ggagggcgag gtgacggagc aattagagtt ctttcccgtc cagagcccgt
    24421 gccggggaat ttgccagtct gacgaacggg gattttgtcg cggctgtttt cgtagccggg
    24481 atgagcgttt taactggaat aaaatgagcg atggcgaaaa acaagaagtg ttgcgccttt
    24541 gtcggcagcg attaatgcgc aaattacgtg caaataagcc agcgtcatca gacgaaccgg
    24601 agcaaccatc actcttttga gcctaatctt gcgtatactc ctctcatatc tgataagagg
    24661 aagcgattat ggttcagcgt attactattg cgccgcaagg cccggagttt tcccattttg
    24721 tgatgggcta ctggcgattg atggactgga acatgtccgc ccgccagttg gtcagtttta
    24781 ttgaagagca tctggatctc ggcgtgacca ccgtggacca tgctgatatt tatggtggct
    24841 atcagtgcga agcggcgttt ggcgaggcgc tgaaactggc acctcacctg cgtgaacgga
    24901 tggaaatcgt cagtaaatgc ggtatcgcga cgaccgcgtg tgaagaaaac gtcattggcc
    24961 attacatcac tgaccgcgat cacatcatta agagcgccga acagtcgcta attaatctcg
    25021 cgaccgatca tctggatctg ctgttaatcc accgaccaga cccgttaatg gatgccgatg
    25081 aagtggcgga cgcctttaaa catctgcatc agagcggcaa agtgcgtcat tttggtgtat
    25141 cgaactttac acctgcgcaa tttgcgctgt tgcaatcacg cctgccattt acccttgcca
    25201 ctaatcaggt ggaaatatcc ccggtgcatc agccgttact gctggatggc acgctcgacc
    25261 aactgcaaca actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt ggtcgtctgt
    25321 ttaatgatga ttatttccag ccgctgcgtg atgaactggc tgtggtggca gaggagttaa
    25381 acgcgggctc gattgaacag gtggtttacg cctgggtatt acgtttaccg tcgcagccgc
    25441 tgccaattat cggctcaggt aaaattgagc gcgtacgggc agcagtcgaa gcagaaacac
    25501 tgaaaatgac ccgtcaacaa tggttccgta tccgtaaagc ggcactaggt tacgacgtac
    25561 cgtaagcact tttaggaata gccgccgttc aaaaatgtgt cactggttta cacttattca
    25621 ggaatgcaca atgaacggag gtcctatgaa acgttttagt ctggccattc tggcgctggt
    25681 tgttgcgacc ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg tcacgtcgca
    25741 aggggttggg cagtcaattg gtagcgtcac cattactgaa accgataaag gtctggagtt
    25801 ttcgcccgat ctgaaagcat tacctcccgg tgaacatggc ttccatattc atgccaaagg
    25861 aagctgccag ccagccacca aagatggcaa agccagcgcc gcagaatccg caggcgggca
    25921 tcttgatcca caaaataccg gtaaacatga agggccagaa ggtgccgggc atttaggcga
    25981 tctgcctgca ctggtagtca ataatgacgg caaggcgacc gatgccgtca tcgcgcctcg
    26041 tctgaaatca ctggatgaag tcaaagacaa agcgctgata gtccacgttg gcggcgataa
    26101 tatgtccgat caacccaaac cgctgggcgg tggcggtgaa cgctatgcct gtggtgtaat
    26161 taagtaatta agaggccagc gtaccttgcg gcggtgcttg ctcaagttgc gaaagcgagc
    26221 agtacagccg ccagacaatt gccgccagtt ctcgggcggc aggttgatga tgacgggcaa
    26281 ggctgtcgca aatccgctgt aattcttcaa gtgtggccgc cagtgatttt tgctgaacgc
    26341 cacgctcact catcactccc cgcaacagtg aaatacagtt atcccgtact cgcgataacg
    26401 gatcggaacg cgattcccag tcgcgcaatt gccagacaac atgagaacag ttcagcaaca
    26461 ctacacccca gcgtaataac caacggcgag ccagcgcatc ctggctgtta ctcaactgac
    26521 tgacgtgatg ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta
    26581 gttgatcgac aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg
    26641 atcctggacg cagaatggcg aacgctaacc acgccaacgc gacgccaaca atttttgcca
    26701 gattatcgtt aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg
    26761 aacccataaa aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa
    26821 gaagctgcat tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa
    26881 tctgcaccat caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc
    26941 gcatcagcag tgacaaagac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa
    27001 ttgctgccag cgttaaagca ttggcaccgg catcccattg cgaagcaata ctccatgcgc
    27061 caatcatcat caacgtacaa aatgtacgca gcccgctcca catagcttcg gcgttatcgg
    27121 tatgacgagc caggccggat gtccgtggga gtctggcatg atcatctaca ccgctttgta
    27181 gacgatgtaa ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc
    27241 agaaagcgat gtgccggtag tcggtgacgt tggtcgggcg tagtggggag ataatacgtg
    27301 cgacggtgta aacatctgtt tgcgaactgg cgagcgccgt cagcagctgt tcgagaattt
    27361 ctcgcgtggc atctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga
    27421 cactggtcat acgtaattgc tggtggagca gcgcattaag gcgtgcgttt tgctggcgaa
    27481 aacgatagtg gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa
    27541 tcaccccttc atgtgctgca cgaatggcat cggttgtttc aggccgccag agtaaactgg
    27601 catgttccag taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg
    27661 aactgctcgg caggatcatc atcatgccgc cgcacaaaat accgactatc acctcgcaaa
    27721 cacgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg
    27781 cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt
    27841 gcgcacaggc ccaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg
    27901 gctcattgag cgtatgtccg gcaagaagca gtgctgcaat ggctccgagc aaactgccag
    27961 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accaccgcag
    28021 ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca
    28081 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccctgaacc
    28141 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
    28201 ggtggtgccc gacaccagcg tgacatcgcc tgggacttta tccagcgtaa agcggacggg
    28261 aacgcgttgg gcgaggcgaa cccaggggac gttaggtttc acatccggaa taaggcttga
    28321 gtcactttca acgctctgat cataaatcgc ccgaccgata ctggacacgt gaccctgtaa
    28381 cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa
    28441 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcaacaa gggcaaacag
    28501 cggtttcccg gtgtcagcgt agtcgccgat gcgggtagtg agattagtca cccatccact
    28561 caccggggcg cgaatttccg tttgcgccag ttgccattgt gcctgtttca gcgtcgcctg
    28621 tgcggcatca acgctggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
    28681 ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
    28741 gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg
    28801 cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agctggttgt cttttatatt
    28861 cagctcaaca atacgtcccg acacctgtgg ggtgatagaa acctgttctg cgcgtatttt
    28921 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
    28981 cgcaactacc gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac
    29041 cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt
    29101 aacaagggat gccagatgtc accagcgtag atccagccac gaagcaggcg gtgtatgaca
    29161 agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaagta gacggacgca
    29221 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
    29281 attcctttac gacagaatat attgcgttag attaataaat attctttaag tgcgaaaaat
    29341 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
    29401 tagcaataca attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
    29461 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
    29521 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
    29581 gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
    29641 tggcatcgag cagccatcac tggtccgtac gctggaccaa ctggaagaaa aagggttaat
    29701 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
    29761 agagccactg atcagcgaaa tggaggctgt tattaacaaa acccgcgcgg aaattttaca
    29821 tggcatctcc gcagaggaac tggaacaact gattaagctc atcgcaaaac ttgagcataa
    29881 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
    29941 acacgcaaac ttatttagcg cggggaaacg gtcacctgac tgccattgct ggccagtacg
    30001 acacgttggc ctggagagaa atgagtgttc ccttgtttct gtaccaccat gatggtgttg
    30061 ccatcatctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg
    30121 ccctgaccgg ctacaccacc tgcaacggcc cctgctgcag tagccagaga acgcccggtt
    30181 ccgccaccaa tagtatttcc caggaaacca ccaagtacag caccgccaat tgcgccgata
    30241 acgttggaat catcaccgcc ctgaatctgt acctgacgta cattaacgat ggtgccatag
    30301 ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg
    30361 ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc
    30421 attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat
    30481 atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ataatgaaag
    30541 taggatcaac caattataaa caaaatattc atgatgatta ttgatttgaa gataatgttt
    30601 tactaccaac ggtaaaaatt ggtcattccg cgtggtattc acgaaaagtt tatgtttgag
    30661 tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
    30721 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt agcgacaatt gatgaacacc
    30781 gggtcgcaca gctggcaagt ttgagctggc cgatcccggt gtctctgaaa caggctgtac
    30841 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
    30901 tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga
    30961 gagatatcgt tgcgatcggc tgtcacggtc aaaccgtctg gcacgaaccg acgggcgtgg
    31021 caccacacac tttacagatt ggcgataaca atcaaattgt ggcgcgcacc ggaattacgg
    31081 ttgtcggtga ttttcgccgt cgcgatattg ccctgggagg acaaggtgca ccgctggtac
    31141 ctgcgttcca tcatgcgctg ctggctcacc caaccgagcg acgaatggtg ctcaatattg
    31201 gcggcatcgc caatctgtca ctgctcattc ctggccagcc ggttgggggc tacgataccg
    31261 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa ccttacgata
    31321 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
    31381 gcgacccata tttctcgcaa cccgcaccga aaagcaccgg acgcgaatac tttaactacg
    31441 gctggctgga gcgccatttg cgccattttc cgggggttga tccccgtgat gtgcaggcca
    31501 cagtggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
    31561 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
    31621 cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
    31681 tggaagcatt ggcgttcgcc tggcttgcct ggcgaacgct ggcgggatta ccaggaaatc
    31741 tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc cccgctaacc
    31801 cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact tggaacgtta
    31861 gggagggcgt attgccctcc agaccaggaa agtcttcggg atatgtctat gaaaaaactg
    31921 ttactgatct gtttgccggt gcttctcacc ggctgtagcg cgttcaatca actggttgag
    31981 cgcatgcaaa ccgatacgct tgaataccag tgtgatgaaa aaccgttgac ggtcaaactg
    32041 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
    32101 ggcatttcag cgtctggtgc acgttacacc gacggaatct atgttttctg gtcgaaaggc
    32161 gaggaagcga ctgtctataa acgcgaccgc atcgtcttga ataactgtca gttacaaaat
    32221 ccgcagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact gacaatccgt
    32281 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
    32341 caccaaaggc gggttacgcc gccgcgatct acccgccgat ccattaaccc tttttgaacg
    32401 ttggctctct caggcttgtg aagccaaact ggctgaccct accgcgatgg tggtcgctac
    32461 cgtggatgaa catggtcagc cttatcagcg catcgtttta ctcaaacatt acgacgaaaa
    32521 aggcatggtg ttttacacca acctcggcag ccgtaaagca catcaaatcg aaaataatcc
    32581 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
    32641 agcggaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
    32701 ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
    32761 aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccaagttt
    32821 ttggggtggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
    32881 cctgcatgac cgctttttgt accagcgtga aaatgatgcg tggaagattg atcgtcttgc
    32941 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
    33001 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
    33061 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
    33121 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
    33181 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
    33241 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
    33301 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa cactgaagaa
    33361 actgttcagg agtgggtgga caaaatccgc aagcaggttg ccccgttcct cgatttcgac
    33421 tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
    33481 ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa
    33541 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
    33601 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt ggtgctgcaa
    33661 atcggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
    33721 catcagaatc aggtgtttgg cttgaccgtt ccgctgatca ccaaagcaga cggcaccaaa
    33781 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
    33841 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
    33901 accttcatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
    33961 gcaccgcgcg cccagtatgt gcttgcagaa caggtgacac gtctggtgca tggtgaagaa
    34021 ggtttgcagg cggcaaaacg tattaccgaa tgcctgttca gcggttcctt gagtgcgctg
    34081 agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga gatggaaaag
    34141 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
    34201 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
    34261 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
    34321 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
    34381 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccagtctca
    34441 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc gcctgggcgc
    34501 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac
    34561 cggttgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
    34621 taaattacac acctgtgatg ccgtattaag tggctatctg ggatcggcgg agcagggtga
    34681 acatatcctc ggtatcgttc gtcaggtgaa agcagcgaat ccacaggcga aatatttttg
    34741 cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    34801 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg ttgagctgga
    34861 aatactctgt gagcatccgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    34921 cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg gctacagccg
    34981 tgaccgtttt gaaatgctgc tagtcaccgc cgatgaagcc tggcatatca gccgtccgct
    35041 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    35101 ggtgaaactg cttcaggggg caacgctgca ggaggccctg gaacatgtga ccgctgcagt
    35161 atacgaaatc atggtgacca caaaagcaat gcaggaatat gagctgcaag tggttgctgc
    35221 tcaggatcgt attgccaatc cagaacatta tttcagcgca acaaagctct gaaatatctc
    35281 atttcggccc acaacgacct gtgggccgat ggctattact ttaagccttc cgctgacagc
    35341 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgccgcaat gtgctctaac
    35401 ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag ataggcatca
    35461 gcaattgtga atcgttgccc gcagatccag tgctcatcct tcagggcctc gttcacatat
    35521 tgcagcttct tctccagctg cgcgcgaact gtcggtttgt actcttccgg tgtatcaggg
    35581 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    35641 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    35701 gcaagatact gcataatcgc tacgccttcc gtcagcaaag taccatcgtc cagcagcaat
    35761 gcaggtacct gtcccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    35821 ttcattaaat ccacactgac gagcgtaaaa tcctttccgc tctcacgcag ggtgatatgg
    35881 gaagcgagag agcaggcgcc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    35941 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    36001 ggcttgtaaa tgagtttccc tgaattttac gctacggctg ctttcgccgc tttgtctgca
    36061 gcgtcatcct gcgtcatgcg gtgcagtttc ggcgcggtaa acagcatcag tactgcaata
    36121 acggcagtag cgacaccaat ctgcaagaat acgcgaccat agacttccag tgacatcagc
    36181 ggatcggtaa cgttatccgg cacagccatc atacctgcaa cataaccacc aatcaggttt
    36241 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    36301 agttgtgcaa ccattgccag acccaggcca gagatcatca gttccccgat actctgcagg
    36361 ccatagctcg cgaccagcca gcttacagac acgataccag cgtcagacgc gaatttcgca
    36421 cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgatagc aaacttggtt
    36481 ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg gctaccaatg
    36541 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag gcccagaatg
    36601 gtgtgttcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctataaagc
    36661 acgaagaaga taatggcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    36721 gcacctttca tcgcgaaggc ttctttaccg aagataacca caataccgaa ggcaacaacg
    36781 cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat agcgatcagt
    36841 gccacaacac caataatggt cagcagcagg ttacggtagt tgataggctc gaagtctggt
    36901 tttgaaccgt actgtttaac ccaacgttgg cagaaggcga agttaacgat agtgatcagc
    36961 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggc
    37021 gtagcaatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    37081 ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    37141 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacgat accggcgtcg
    37201 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    37261 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    37321 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    37381 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    37441 tcaatcgaga agatgagata gaacgccttc ggttgtttaa aagcgttcaa actgacgctt
    37501 tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac
    37561 gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata gtgggagggg
    37621 aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt
    37681 acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt
    37741 tcaaaaaacg taacggcaga ttttttcact accctgagag ggtgatgatg gcttaacggc
    37801 gatatgttct actatttagc gtttttccta gtgagatagt ccagtttctg aaaaatagcc
    37861 agtgtaatgt tttgtaggtc aatatttgtt gtgtgtgtaa atttaaatga ttttaacctg
    37921 attcttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa tgaatagaaa
    37981 atttcgctat taaaaaaaca attaacctgc atttttggtt atctgcaaac ggattacctg
    38041 atgataacgc taagcaatgg catcattgac gttaaaaaga tgttttaaaa gaggataaag
    38101 aaaggttatg aatggggtaa tcggtgttac ccctgatctt tagatgtcaa ctttctcttt
    38161 gtattcacaa agatcttcaa taatacaaga gccacagcga ggcttgcggg caatgcaggt
    38221 ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg ccggaaccac
    38281 tttcagtagc ttttcttcta cctgttcgac gtttttcccc ggcgcaaatt gagtacgatt
    38341 gcaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgtatg cagtgtttaa
    38401 tacgacgttg gctgttttac gacctacacc aggcagggct tcaagcgcag cacgatcttc
    38461 cggaacctcg ccattatgtt gttccagtaa tatacggcag gttttgatga tattttctgc
    38521 tttgctgtta taaaggccaa tcgttttgat ataggttttc accccttcaa cgcccagttc
    38581 aagcatcgct gcaggcgtat tagccaccgg gtagagtttc gccgtcgcct tattaacact
    38641 gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag gcgaactgaa
    38701 attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga tctccaggcg
    38761 ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg ttcggcagct
    38821 gcttcagtac ggcgcttttt cattttttca tcaatcaggt attttcctgc cagcatcagt
    38881 cccaggccaa taaatgcacc tggtggcagc atcgccagca ggaaagggga gtcggtgtgg
    38941 aaaatctcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc accgtcaaac
    39001 aacgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc gcaggttgcg
    39061 cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt ggcggcgaag
    39121 gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa aatccctaat
    39181 gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct gaccaccgag
    39241 gcgatgatca tcacgtaaat gggaatgcgg atctcggcgg gcgtccagtg acgcagcgtc
    39301 gaaatggtca ggttggttag cgtcagtacc agcgtagtcg caagtcctaa acccagagcg
    39361 ttagtggcag tggacgtgac cgccaacaga ggacaaaggc cgagcagctg gaccagcgca
    39421 gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac gttttattct
    39481 ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa tccggcgcgt
    39541 tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cagtgaactg gtcgaaatca
    39601 ccaccatctt tcttcaccgc ccagttcgca tcatccgcac cactgatttt tttacccgca
    39661 aaatgggtga tccagtcaga aaggcgcagt tcgattttat cgccaagccc tggcgtttcg
    39721 tggtgctcag tcacgcgcgt gccaagtacc gtgccgttaa aatcggctcc caccagcagc
    39781 tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcagc taccggtttg
    39841 tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg cgcagttacc
    39901 agatagcaac tctgtgccag cgcattgtta tagcgttcag ctggcagcac ctgatcaaat
    39961 aacgcctttt gttgcagact ggcctgctca gcaattgtcg ttttggtcat ctggttgatg
    40021 gccgcagtta accctgttga acccgctgca aagagcgcca acgtaatgcc gtgttttcgg
    40081 atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg cgtgtagtaa
    40141 tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc gtcaggatag
    40201 ccgccgaaac tgcggatcaa ccagactaat aagcccgcca gcgcgccgaa aataagacga
    40261 ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa taaagaatgc gccgagcatg
    40321 gtcgcaccag acagcagatg aatttgcggt gctgccagag tatctggtga gaacaaccag
    40381 cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg ccagcgaatc
    40441 gctttctgcc atagcaacca cacgccgcca gtcagccagg cgagatttac ccattgccaa
    40501 ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctcttc aaccgaatga
    40561 ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg cctgactaat gccatcaata
    40621 cctaagcgta gcgtgttcat atcaccacca ctggtggtat gtccgctgaa aataacctgt
    40681 atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa ccagctggtc
    40741 atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg attaaacggg
    40801 ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac cgtacccagc
    40861 acgaccatcc accatggcgc gaggggggga atacttaccg ccagcaataa gcctgtcagc
    40921 aatgctgagt tatctttcaa cgttgcagct accgactgct tgcgtagttt gaggacgaga
    40981 gcttcggcca gaagagcact gaccgacgcc aacaggatct gaacgagagt accccaacca
    41041 aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaataccaa catcataatg
    41101 cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa taccatttag
    41161 tcctcgttta caaccttttg ctgggcggct tttttggcct gaacgcgggc aatagccgca
    41221 gcaactgccg ctttgcgcgg atcaatctgt tcttctggct ctgcattagc ctgttgctgt
    41281 tccagcttgc gtgctttggc acgggcaata gcagcttcga cggcggcttt gcgtggatca
    41341 acctgttctt ctggttccgc attagcctgt tgttgctgtt ccagcttgcg cgctttggcg
    41401 cgtgcaatag ccgcttcgac ggcagctttg cgcggatcga cctgttgttc tggctccgca
    41461 ttggcctgtt gctgttccag cttgcgtgct ttggcgcggg cgatagcggc ttcgacggcg
    41521 gctttgcgtg gatcaacctg ttcttctggt tccgcattag cctgttgttg ctgttccagc
    41581 ttgcgtgctt tggcacgggc aatagcagct tcgacggcgg ctttgcgtgg atcaacctgt
    41641 tcttctggtt ccgcattggc ctgttgctgt tccagcttgc gcgctttggc gcgtgcaata
    41701 gccgcttcga cggcagcttt gcgcggatcg acctgttgtt ctggttcggc attagcctgt
    41761 tgctgttcca gcttgcgtgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc
    41821 ggatcaacct gttgttctgg ctccgcattg gcctgttgct gctccagctt gcgtgctttg
    41881 gcgcgggcaa tagccgcttc aacggcagtt ttacgtggat cagcaacggt tgcagcgtcg
    41941 ttagtttgct gcagttctgc ctgtttcgct ctggcttgcg ctttacgggc ttcccgtgct
    42001 gcaataattg cactgttatc cgggcgttcg cccgctttaa tcacaatagg ctgtgtagcc
    42061 tgggcctgtt tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt atctttggct
    42121 gcaggttgaa cggctgcgct cttatgtcgt tcaaggcgag ccgttttttc gcgctccaga
    42181 cgagcctggc gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc ttcctgacga
    42241 atagccgcaa tttcagcttt ttcctgacgg aaatattgca ccagggggat attgctcggg
    42301 caaacccacg cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt ggtggcttta
    42361 tcgtgttgct gacctttgct gaaccagtac agctgttgcg gcaaaagatc cgcagggcag
    42421 gcgtcagcac aggcgctaca acggatgcaa ctttgttctt cctgtggttc gccaagttca
    42481 ttggcagagg gagccaaaag acagttggta atctttacga ccgggacatc cagccacggt
    42541 aaggtaaagc ccattagcgg gccgcccata atcaccattt gatcggcaga ggggcagaat
    42601 ccggcatcat tcaataaatg acgcactggc gtacccagcc gtgcccagac gttgcccggg
    42661 cgagcgattg cttcgccagt cagggtcacg acacgctcgg taatcggctc gccatcaata
    42721 acggcacgtt tcactgcata agcggtgccg acgttttgca ttaatacgcc gatatcggat
    42781 gaacgcccgc catgtggaac ctgcttcccg gtcagaatgt aggttaattg tttagcaccg
    42841 ccagaaggat atttggtggg aatcacccgc agagaaatat cgtgggagtc cgccagcacc
    42901 gcgcgtagca tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag aatttcgcgc
    42961 ggctgcagaa tatgcgcaag gatgcggata ccctctacga cctgagccgc gcaatcctgc
    43021 atcaaacggt catcggcggt aatgtacggc tcgcactcag cagcgttgat aatcaacgtt
    43081 tcaatcttat ctccgccgcc ctgcaattta acgcctgtcg gaaatcccgc accacccagc
    43141 ccggcaacac caaactgatg aatgcgctcg attaactctt cgcgactgcg agagcgataa
    43201 tcggcccagc cgtcgcgcgg gatccagcag tcttcgccat cggcatcaat aatcacactt
    43261 aattcagcta aagctgaagg atgagccgta gagtggggcg caatagccgt aacggtaccc
    43321 gaggtgggcg cgtgaaccgg cagcattttg ccgcgaccac gggtaagcgg ctggccgcgc
    43381 aatactttat cgccgacgct aacgcacaac tcaccttcag cgccaatatg ttgttttagt
    43441 ggaatgacaa aacgctgcgc cagtggtacc tggcgcaggg gcgtaccgtt ggactgggtt
    43501 ttcatctccg gtggatggat gccgccgttg aaatcccaga ttttattttt tctgaatgca
    43561 gagaataact taagcatggt gttccacggg aatgatacgc acgggaatgg tgttcagatc
    43621 ccatttccag gagtcaggag tttctgcgac cggttgcaac gagatgcagt gcgtcgggca
    43681 cggatcaaca cataaattgc agcccgtaca gagatcactc attaccgtat gcatggcgcg
    43741 ggtagcgcca acgatggcgt ctaccggaca cgcctgaata catttagtgc agccaataca
    43801 gttattttca tcaataaccg ccaccatccg cgctggcgtt agctcttgcg cttcgccatc
    43861 cagcggctgc ggctcgacat taagcaactc ggcaattttt agcatcacag cttcgccacc
    43921 tggggcgcaa cggttgattt tttcaccgtt acagctgatg gcttccgcgt aggggcgaca
    43981 gccgggataa ccgcactgac cacactggct ctgcggtaag atttcgtcaa ttttctcaac
    44041 gaccggatcg tcttccaccg caaaacggcg ggaggcataa cccagaatgg cgccaaacgc
    44101 cagggccagc aggctcacgg cggcaacggc aatccagata gcattcatta caacttcacc
    44161 aaaccactaa agcccataaa ggccagagac ataagacctg cggtaattaa cgcaatggca
    44221 ttaccgcgaa aaggtgccgg gacatcagcc acagcaaggc gttcgcggat ggcggcgaag
    44281 agcaccatca ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc tgactgcaag
    44341 aaattgtgcc cgagattgat attcagcaac gccacgccga gcacggcaca gttggtggtg
    44401 ataagcggca aaaaaatccc cagcagacgg taaagtaccg ggctggtttt gcgcaccacc
    44461 atttcggtga actgcacaac cacagcaatc accagaataa acgccagggt gcgcagataa
    44521 atcagattaa gtgggatcaa aatccacgta tcgataagcc aggcgcaaat agacgccagc
    44581 gtcattacaa acgttgttgc cagccccatg cccatcgcgg tttccagctt tttggaaacc
    44641 cccataaacg gacagagacc gagaaacttg accagtacaa agttattgac cagtacagtt
    44701 ccgacaaaca gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa gccgcctatt
    44761 atccgttaat ccagagcagg cgacaacagg ttaactgtaa ggttattacg gattcacaaa
    44821 cgtcgctttc acccgcgacg agcgtttgaa ataggggacg atcaatgcgg ttgccaacaa
    44881 agtaaacagc aactggcgaa cggctatgcc atcttctacg ggtgaaaaag cgaacgcttt
    44941 tactgccagc aatacggaaa taagcagcca gataatatag tgtttaggaa cacaacgccg
    45001 acgtttaaag aaagcgatgg tcaaccacag tgtgtaatac cacatggcga tagcggtaat
    45061 aaaagaaacg ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg tttgaaatgt
    45121 ttgcggagaa gataacgcag cggtatacag caacaacgcc agcgtcgtac tcaacagagc
    45181 gactaaaagc caggccaacg ggccaagcaa ccagcctcca atacgttgtg gcgttgtggt
    45241 ggtcataggt tctccagaat agcgacgcaa acagacaaat ctaattttag gggcgttatt
    45301 ataaggcttt tataaccgat cgctactttc tttactggac atagtgccag acggacttcg
    45361 gtacctggcc gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga cggtgatcgg
    45421 cagcacgata catattgatc agttcctgat cgtccgtcag ggtgtaatta agatggtcgt
    45481 agagtttttc cagactttcg agtgaactga ttttgcgaaa ttttaataag tagtcctgaa
    45541 cagtcataaa aggtccataa ataattaata ccaaaggggt aggtattatt agtgatgata
    45601 aaatttcacc aaaagtgaac gcttgtgtta ttacaataat aagaatgcgc tgatggcaaa
    45661 tattcaggga tgaaaaactg ctctgaaaag agtaacgcgc tttactatcc ctgacaatca
    45721 tcaacaacat cgaacaagat aataaattcc tggtttaata tccgacaagt gaaaacatgc
    45781 acccggacgg gcagcatgtc gctcaacaag tgcagagctt acttgtgttg taccgtagta
    45841 ctctgttcag gtggctgata gttgtcaatg tgactcgcca cgccaagaag aatgactgaa
    45901 acgacaagaa cgatccaacc tgttaattca ataagacgat tcattacagc ccacatctct
    45961 gttgattgat ccattaactt cagggggtaa atgttactta gcaataatag ctccagcaag
    46021 atttttactg aggtttttgc gatatttagc ttttgtcgtt ggaaaaatcg ctattttttt
    46081 gacttaagtt aaacaacatc ccttattgct ggcaggttat taaactgttg agcgagggta
    46141 aaggatagtg tcaaatagcc atcatacttc aatgagaggc aatgacatga gcgacaacat
    46201 ccgtgttggg gtgattgggt atggttatgc gagcaaaacc ttccatgcgc ccctgattgc
    46261 gggcacgccc gggctggaac tggcggtaat ctccagcagc gatgaaacaa aagtaaaagc
    46321 cgactggcca acggttacgg ttgtctctga gccgaagcat ctgtttaacg atcccaacat
    46381 agacctgatt gtcattccta cacccaacga tacccatttt ccgttagcta aagcggcgct
    46441 tgaggcgggt aaacatgtgg tcgttgataa accctttacc gtgacactgt cacaagcacg
    46501 agagctggat gcgctggcaa aaaccctggg gcgtgtgttg tctgtattcc ataaccgtcg
    46561 ctgggatagc gattttctga ccctaaaagg tttgctcgcg gaaggcgtgc tgggggaagt
    46621 tgcttacttt gagtctcatt ttgaccgctt ccgtccgcag gtacgcgatc gttggcgtga
    46681 acagggcggc ccaggcagcg gtatctggta cgatttagca ccgcaccttc ttgatcaggc
    46741 cattacgcta tttggtttac cggttagcat gacggtagat ttggcacagt tacggcccgg
    46801 agcgcagtcg accgattatt tccacgctat cttgtcctat ccgcagcggc gagtgatttt
    46861 acacggcacc atgctggcag ccgctgagtc agcacggtat atcgtgcatg gatcccgagg
    46921 cagttatgtg aaatatggcc tcgatccaca ggaagaacgt ctgaaaaatg gcgagcgtct
    46981 gccgcaggaa gactggggct acgatatgcg tgatggcgta cttacccgcg tggaaggtga
    47041 ggaacgtgtc gaagaaacgc tgttgacagt gccggggaat tatccggctt actatgcggc
    47101 tattcgtgat gcgttaaatg gcgatggtga aaatccggtt ccggcaagtc aggcaatcca
    47161 ggtaatggag ttgattgagc tgggcatcga atccgccaaa catcgcgcga ctttgtgcct
    47221 tgcgtgaaaa ggggatggtg caattagcac catccatttt tgttacttcg cggcgacttt
    47281 ttctcgcagt gcgcgttttt cctctgcgct gaggaaagcc atttccagac cattaatctg
    47341 tgcctggcgg atttgctcgc gggataaccc agcagctggc gcggcaacgg tatattcgtg
    47401 aatgatatcc actccctgca cgccgggatc gtcagtgtta atgctggcac gaatgccatg
    47461 ctcaaggaac gttttcagcg gatgtgcagc cagatccgcc acggtgctgg tctgaatatt
    47521 ggaggtcaga caggattcaa taccaatttg ttgctcggcg agaaaatcca tcagcgcccg
    47581 atcttcaatg gcttttacgc catgtccaat acgctccgcc cccagttcac gaatcgcctg
    47641 ccagatgctt tccggtccgg cagcttcgcc tgcatggacg gtaatatgcc agcccgcatc
    47701 acgcgcgcgg ttgaaatgag aaaggaacag acttcccggg aaaccaagtt catcaccggc
    47761 taaatcaagt gcggtaatct ggtcacggtg ggctaaaaag gcctccagct cttgctgaca
    47821 ggcggcttcg ccgaaggtcc ggctcataat accgataagc ttcgcctgca caccaaaggt
    47881 gcggcaacct tcacgtacgc catcgatcac cgcttcgaca acacccgcta caggcagctg
    47941 atgtgccatt gccatgtagc ctggtgaaaa acgcagttcg acatagtgca ggccgttacg
    48001 ggctgcatct tcaatgtttt caaatgccac gcggcggcag gcatcaagag aggcgagaac
    48061 tttaacgccc cagtcaagtt tagtcagaaa gctcaccaga tcgggttcgt tggcaatgac
    48121 ctgaacgtgg ggagtcagtg tttccaggga ttgtgcagga agcgagatat tatactggcg
    48181 gccaagttca agaatggtct ggggacgaat gttgccatca aggtggcgat ggatatcagt
    48241 taatggcagg gtggtatcaa tcatggtcgc actctttttc tggatataaa agtgcgcacc
    48301 attataaaaa caaagcaggg taaaaatcca cgattgcgca acgatttttt acgttgcgca
    48361 attggggtta accaacagcg cggatggcgt taattaatcc agccacacct ttttccagtt
    48421 tcgaacgtgg gcagccggca ttgagtcgga caaaaccacg accttcttca ccgtaggtat
    48481 atcccggcat gatcgcgatc ttttcttgct cgataagcgc tttttgcaac gcgttgtcgt
    48541 caatattcaa cggacgtaga tcaagccagg ccagataagt ggattgcggg atctgccagt
    48601 tgagttcagg aaacgcagcg ttcattttat ctgcgatata cgtcaggtta tctttcagat
    48661 agacgcgtaa ggcatccagc catggcgcgc cttgctgata ggcggcgata tgagcagtta
    48721 acgccagtac cgaaggggaa gaaagcccat cacgtccttt cagtgccgat aaataggcat
    48781 cgcggctact gctattttct ataatcccgt aagcaccggt cagggcggga atattgaaac
    48841 tttttgatcc cgacgttagc aacgcccagt ctccgcgcgc cacattactc cagggaatat
    48901 gcggctgctc gccccaaacc atatccatat ggatttcatc ggaaataacc cgcacaccat
    48961 gacgctcgca caggtcagcc atgatctcca gctcatcgca cgtccacact ttcccggtag
    49021 gattctgtgg gctacacagg agcataattt tacattctgg tttcgccagc acggcttcca
    49081 gcttgcccat atcgcaaaac cagccgtcag cctgcttctc taaagcaacg ggcattactg
    49141 tgcgctggtt accttcaatg gccttgtaaa atgcgtcata ggcgggtgtg tggatcacca
    49201 cgccttcacc tgtttcagac cactgacgaa tcagttctga aaccatatag ataacagaag
    49261 ggccatacac caccgactga ggatcgatgg cggtgtaatg ctgggtggaa aaccagtggg
    49321 caatagccgc gagaaactca tcgtttttcc agcggctgta gccaaacacg ccgtgtatca
    49381 ggcgctgatt cagcgcctcg ataatgcagg gggcagtggc aaaatccatg tctgaaatcg
    49441 tgaacggtaa caggtcagca gtgccgaaac ggtcagcgac ataatcccac tgcgtacacc
    49501 atgtgccatg acgatccacg acctttgaaa aatcgaacat atcttatcct tatgcctgga
    49561 cagtacgcat cagaccgacc atttcatctt ttactgactg aacttgtggc ccgataacaa
    49621 cctgcaggtt atgttgatta agttgtacta cgccaattgc ccgattgtcc ttcagtgcct
    49681 gcacattaac aagcgacata tcttttacag acaaacgcag acgcgtaatg cagttatcga
    49741 gactgacaat attgtcggca ccgcctaagg cttcgaggat tgccggaacg ttgtaacctg
    49801 atttacccgg cgcaccggca acggcttttt cgattgaact ggcaacttca ctatcgcgcc
    49861 ccggggtttt gagattgaag cgggtgatag cgaaacggaa gatgatgtag taaacgacaa
    49921 accagattgc cgccactact ggcaccatgt accacttggt tgacagacca tgcaaaatac
    49981 cgaacaccac aaagtcgatg atattgccgt cggtattacc gatggtgacg ccgagcacag
    50041 acatgacagt gaagccgagg ccggttaaca gcgcgtggat gacatacaga actggcgcta
    50101 cgaacaggaa caggaattcc agaggttcgg tcgtgccgcc aacgacacag gcgatcaggc
    50161 cagaaatcag cagaccttta attttatggc gattttccgg gcgcgcacag tgatacatag
    50221 ctaacgctgc acctggcaga ccgccgagaa acgcaggcat tttaccttgc gaaaggaaac
    50281 gcgtggcgct ttcagaaaaa ccgtgagtgg tcgggcaact caattgcgcc tggaagatgg
    50341 tcagtgcacc gctgacggtt tgaccgcaga cttcctgcgt gccgcctgcg tcggtaaagc
    50401 gaattaatgc caccagaatg tgatgcagac caaacggcaa cagcagacgt tcaccggtac
    50461 caaacagcat cggtccgaaa tcacccgcgc tgttgatcat atggcctaag ccgctaatac
    50521 ccatggcgaa aatcggccag actaatggaa tcaccaggcc gacaagaccc atcaccagcg
    50581 aggagatgat tggtac
//
LOCUS       DAJOTB010000015        49937 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJOTB010000015 DAJOTB010000000
VERSION     DAJOTB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.015"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..204)
                     /locus_tag="OHN28_000900"
     CDS             complement(<1..204)
                     /locus_tag="OHN28_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCQ2296338.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEF"
     gene            complement(267..1739)
                     /gene="der"
                     /locus_tag="OHN28_000901"
     CDS             complement(267..1739)
                     /gene="der"
                     /locus_tag="OHN28_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCQ2296339.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1857..3035)
                     /gene="bamB"
                     /locus_tag="OHN28_000902"
     CDS             complement(1857..3035)
                     /gene="bamB"
                     /locus_tag="OHN28_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCQ2296340.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3046..3666)
                     /gene="yfgM"
                     /locus_tag="OHN28_000903"
     CDS             complement(3046..3666)
                     /gene="yfgM"
                     /locus_tag="OHN28_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCQ2296341.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3684..4958)
                     /gene="hisS"
                     /locus_tag="OHN28_000904"
     CDS             complement(3684..4958)
                     /gene="hisS"
                     /locus_tag="OHN28_000904"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCQ2296342.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5069..6187)
                     /gene="ispG"
                     /locus_tag="OHN28_000905"
                     /gene_synonym="gcpE"
     CDS             complement(5069..6187)
                     /gene="ispG"
                     /locus_tag="OHN28_000905"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCQ2296343.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6214..7221)
                     /gene="rodZ"
                     /locus_tag="OHN28_000906"
     CDS             complement(6214..7221)
                     /gene="rodZ"
                     /locus_tag="OHN28_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCQ2296344.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7506..8660)
                     /gene="trmG/rlmN"
                     /locus_tag="OHN28_000907"
     CDS             complement(7506..8660)
                     /gene="trmG/rlmN"
                     /locus_tag="OHN28_000907"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCQ2296345.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8810..9241)
                     /gene="ndk"
                     /locus_tag="OHN28_000908"
     CDS             complement(8810..9241)
                     /gene="ndk"
                     /locus_tag="OHN28_000908"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCQ2296346.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9390..11702)
                     /gene="pbpC"
                     /locus_tag="OHN28_000909"
     CDS             complement(9390..11702)
                     /gene="pbpC"
                     /locus_tag="OHN28_000909"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCQ2296347.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11703..16664)
                     /gene="yfhM"
                     /locus_tag="OHN28_000910"
     CDS             complement(11703..16664)
                     /gene="yfhM"
                     /locus_tag="OHN28_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCQ2296348.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16871..17716
                     /gene="sseA"
                     /locus_tag="OHN28_000911"
     CDS             16871..17716
                     /gene="sseA"
                     /locus_tag="OHN28_000911"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCQ2296349.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18209..18985)
                     /gene="sseB"
                     /locus_tag="OHN28_000912"
     CDS             complement(18209..18985)
                     /gene="sseB"
                     /locus_tag="OHN28_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCQ2296350.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19127..20410)
                     /gene="pepB"
                     /locus_tag="OHN28_000913"
     CDS             complement(19127..20410)
                     /gene="pepB"
                     /locus_tag="OHN28_000913"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCQ2296351.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20469..20669)
                     /gene="iscX"
                     /locus_tag="OHN28_000914"
     CDS             complement(20469..20669)
                     /gene="iscX"
                     /locus_tag="OHN28_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCQ2296352.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20681..21016)
                     /gene="fdx"
                     /locus_tag="OHN28_000915"
     CDS             complement(20681..21016)
                     /gene="fdx"
                     /locus_tag="OHN28_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCQ2296353.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21018..22868)
                     /gene="hscA"
                     /locus_tag="OHN28_000916"
     CDS             complement(21018..22868)
                     /gene="hscA"
                     /locus_tag="OHN28_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCQ2296354.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22885..23400)
                     /gene="hscB"
                     /locus_tag="OHN28_000917"
     CDS             complement(22885..23400)
                     /gene="hscB"
                     /locus_tag="OHN28_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCQ2296355.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23496..23819)
                     /gene="iscA"
                     /locus_tag="OHN28_000918"
     CDS             complement(23496..23819)
                     /gene="iscA"
                     /locus_tag="OHN28_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCQ2296356.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23836..24222)
                     /gene="iscU"
                     /locus_tag="OHN28_000919"
     CDS             complement(23836..24222)
                     /gene="iscU"
                     /locus_tag="OHN28_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCQ2296357.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24250..25464)
                     /gene="iscS"
                     /locus_tag="OHN28_000920"
     CDS             complement(24250..25464)
                     /gene="iscS"
                     /locus_tag="OHN28_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCQ2296358.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25576..26064)
                     /gene="iscR"
                     /locus_tag="OHN28_000921"
     CDS             complement(25576..26064)
                     /gene="iscR"
                     /locus_tag="OHN28_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCQ2296359.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26335..27075)
                     /gene="trmJ"
                     /locus_tag="OHN28_000922"
     CDS             complement(26335..27075)
                     /gene="trmJ"
                     /locus_tag="OHN28_000922"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCQ2296360.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27194..27997
                     /gene="suhB"
                     /locus_tag="OHN28_000923"
     CDS             27194..27997
                     /gene="suhB"
                     /locus_tag="OHN28_000923"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCQ2296361.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28142..28996
                     /locus_tag="OHN28_000924"
     CDS             28142..28996
                     /locus_tag="OHN28_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2296362.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29187..30467
                     /gene="csiE"
                     /locus_tag="OHN28_000925"
     CDS             29187..30467
                     /gene="csiE"
                     /locus_tag="OHN28_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCQ2296363.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30459..31598)
                     /locus_tag="OHN28_000926"
     CDS             complement(30459..31598)
                     /locus_tag="OHN28_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCQ2296364.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31968..32390
                     /locus_tag="OHN28_000927"
     CDS             31968..32390
                     /locus_tag="OHN28_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2296365.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32438..33310)
                     /locus_tag="OHN28_000928"
     CDS             complement(32438..33310)
                     /locus_tag="OHN28_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCQ2296366.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33322..34416)
                     /locus_tag="OHN28_000929"
     CDS             complement(33322..34416)
                     /locus_tag="OHN28_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2296367.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34449..35447)
                     /locus_tag="OHN28_000930"
     CDS             complement(34449..35447)
                     /locus_tag="OHN28_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2296368.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35450..36979)
                     /locus_tag="OHN28_000931"
     CDS             complement(35450..36979)
                     /locus_tag="OHN28_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2296369.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(37002..37985)
                     /locus_tag="OHN28_000932"
     CDS             complement(37002..37985)
                     /locus_tag="OHN28_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2296370.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38082..41363)
                     /locus_tag="OHN28_000933"
     CDS             complement(38082..41363)
                     /locus_tag="OHN28_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCQ2296371.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41481..42674
                     /locus_tag="OHN28_000934"
     CDS             41481..42674
                     /locus_tag="OHN28_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCQ2296372.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42738..43991)
                     /gene="glyA"
                     /locus_tag="OHN28_000935"
     CDS             complement(42738..43991)
                     /gene="glyA"
                     /locus_tag="OHN28_000935"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCQ2296373.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44320..45510
                     /gene="hmpA"
                     /locus_tag="OHN28_000936"
     CDS             44320..45510
                     /gene="hmpA"
                     /locus_tag="OHN28_000936"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCQ2296374.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45555..45893)
                     /gene="glnB"
                     /locus_tag="OHN28_000937"
     CDS             complement(45555..45893)
                     /gene="glnB"
                     /locus_tag="OHN28_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCQ2296375.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(45954..47288)
                     /gene="glrR"
                     /locus_tag="OHN28_000938"
     CDS             complement(45954..47288)
                     /gene="glrR"
                     /locus_tag="OHN28_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCQ2296376.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRNELDANDFKE"
     gene            complement(47278..47991)
                     /gene="qseG"
                     /locus_tag="OHN28_000939"
     CDS             complement(47278..47991)
                     /gene="qseG"
                     /locus_tag="OHN28_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCQ2296377.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48156..49583)
                     /gene="qseE"
                     /locus_tag="OHN28_000940"
     CDS             complement(48156..49583)
                     /gene="qseE"
                     /locus_tag="OHN28_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCQ2296378.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT        11736 a        13953 c        12390 g        11858 t
ORIGIN      
        1 aaattcaacg cgttttttag atattaaacc gtgcccatgt tggcagaaat aatccacagc
       61 accacaagct tttaacactt gtaacggctg atgacactca gggcaaagtg cctggacggt
      121 aaagtctttg tcgcaagttg cacagtgagc agtctcgcca ttgcgttcga gaggggtttt
      181 gcaggttgga cagttgagtg ccataaatac tcctcgcgtg ttattcggtt tccctctccg
      241 tgtggaaagg gaaaccagat aagtgattac ttacttttct tgatgtgctt catcagacgc
      301 ttacgcttgc gcatctgagt cggcgtcagg gtattacgct tgttcgcata cgggttttcc
      361 ccttctttga actgaatacg aatcggcgat cccattacgt ccagggattt gcggaagtag
      421 ttcatcaggt aacgcttgta ggaatcaggc aggtctttca cctgattgcc gtgaatcacc
      481 acaatcggcg ggttataacc cccggcgtgg gcgtatttca gcttcacacg acgaccgcgt
      541 accagcggcg gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc
      601 cccacacgac gggtggagct gtcatacgct tcacgtactg attcaaacaa gtttccaaca
      661 ccactgccgt gcaaggcaga gataaagtgc acacgagcaa aatcgataaa gcccagacgg
      721 aagtccagcg tctctttcac ctgctctttc acttcctggc tcaggccgtc ccacttgttc
      781 actacaatca caagtgagcg cccactattg agaataaagc ccagcaagga gagatcctga
      841 tcggaaatac cttcgcgcgc atcaatcacc aacatcacca cgttggcatc ttcaatggcc
      901 tgcaacgttt tgattaccga gaatttctct accgcatcgg tgattttgcc gcgtttacgt
      961 acgccagcgg tgtcaatgag cacatactca cgtccgtcgc gttccattgg aatgtagata
     1021 ctgtcacgcg tcgtgccagg catgtcgtaa acaacaacgc gctcttcacc aagaatacgg
     1081 ttagtgagtg tagacttacc tacgttcggg cgacccacaa tcgccagttt gatcggcaga
     1141 ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc
     1201 gcccagtatt cggcgtcttc gtcgacttct tcttgcggtg cgagatcttc catccacggc
     1261 agcagcacgt gctccagcag acttaatacg ccacgaccgt gagacgcggc gatcgggtag
     1321 atttcaccta aaccaagcgc gtagaaatca accactgcct gatcgggatc cagaccgtca
     1381 gttttgtttg ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt agcaatcgct
     1441 tcatccgccg gcatcaggcc cgcgcgcgca tccaccataa acagtacgac gtccgcttct
     1501 tcaatcgcca gcagcgactg ttccgccatg cgggtttcta caccgtcttc tgtgccatca
     1561 atcccgccgg tatcaatgca gataaactca cggccttcaa tttccgcacg accgtactta
     1621 cggtcacgag tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg
     1681 ttaaataacg tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg
     1741 tttaaagcct catttttata aatcatcaga caacgcacgc tatattcgtg tcgttgttaa
     1801 aaacaggaaa acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa
     1861 cgtgtaatag agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcagca
     1921 accggttcag tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct
     1981 tcgacgttaa tccagtgcag ataaccttca ctgtcgccta ccaccaggtt gccattatac
     2041 agcaccggag aagtcagcag gcgatgcagc agatcgcttt gtgtccacag cgtaacgccg
     2101 ccatcaatgg tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgccg
     2161 tcgacgatga aatcattcac cgaacccagt tcgcgtttcc acataatctg accactgcgc
     2221 agatcaagcg ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg
     2281 acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa
     2341 atacgctgct gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta
     2401 tcgccaccca cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag
     2461 agcgaaggca tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct
     2521 tgtaactgac cgttactggt gtggattaac accagaccgt cgctgaccac cgggcgcgaa
     2581 agtgcttcac ccgcgacttt agtttgccat gccacagtac catcgctggt attcagcgcg
     2641 taaacctgcg ccttttcggt gccaatgtag acatgcccac cagacacggt cacgccgcca
     2701 gaaagtaatg caggatcttt agagaaccag ccatctttct cggccaggtt gacagaccag
     2761 atttctttgc cgtcatccgc attcagcgct tttactaaac cagcgcggtc cgctgcatag
     2821 acaacgttgt ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca
     2881 acggaagtgc tccacacagt agtcggcgta aactggtttt caacggttgg caatggggac
     2941 atctttacca catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa
     3001 agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt
     3061 attaattttc atctgcatca tttcgctcag tgccggggta acatcgcttt tcacgcctgc
     3121 ttcccatgca ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag
     3181 gtcggcaaca atggcagccc acccttcacc tttgatagca tcaagggttt ttagcgcggc
     3241 atcagcctgc ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag
     3301 attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc
     3361 atttttgtca acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt
     3421 attttcagca gcaaattttt ccgccgctgg gatgctatcc ggtttgcctt cgctcactgc
     3481 ggtaaccgca ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctatt
     3541 ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt
     3601 gccattttca gcaaaaaagc gtttaaccgc ttcaacctgg tcgttttcgt tctcgtaaat
     3661 ttccacgctg tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta
     3721 tcctgcgcaa ctgcagtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta
     3781 gccacttcag actcacccag caccacagca acgcgggcac cccatttatc agcacgggca
     3841 aactgtttct taaagttgcc gccgccgtgg ttggtcatca gtttcacacc cggtaattca
     3901 tcacgcagac gctctgctaa tgccatagcc gcagattgtg tgtcagcacc tgaagccacc
     3961 aggtatatat cgacaacagg atcggcttta aattccggat taacggcctg aactaacaat
     4021 acaagacgtt cgaggcccat cgcaaaaccg actgccggtg ttgcacgacc acccagttgt
     4081 tccaccagac cgtcataacg accacctgca cacacggtgc cctgggaacc gaggctgtta
     4141 gtcacccact cgaaaacggt acggttatag taatccagac cgcgcaccag acgctggttt
     4201 acagtgtaag cgatccccgc gctctcaagc agtttgcaca gaccggcaaa gtgctcacga
     4261 gattcctcgt ccagataatc gcctaatgcc ggagcgtcgt tgagaagcac ctgcacttcc
     4321 ggattttttg aatccagtac gcgcaacggg ttagtgtaca tgcggcgttt gcagtcttcg
     4381 tccagctttt ctttatgctg ctcaaggaat gccaccagcg catcgcggta attcgcacga
     4441 gcttccagcg aaccgataga gttcagctca agagttacgt gctcggaaat acccagcgcg
     4501 cgccaccagc gggcagtgag cataatcagt tcggcgtcga tatccggacc ttgcagaccg
     4561 aaaacttcgc agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga
     4621 cggaacatag gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg
     4681 atgccggcgc gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg
     4741 cgatcctcaa aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt
     4801 ttgaatagcg gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg
     4861 agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa
     4921 tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata
     4981 aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc aatcatacac
     5041 gggaagcgag gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac
     5101 gcgcttcgtc cagctgactg gctttcgcac gaatgcgcgc ttccagctga tcgatcatat
     5161 cgttgttgtc cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc
     5221 cgccagtgac gccgagtgta gaaaccagcg cctcacctgg gccattcacc acgcagccga
     5281 taatcgaaac gtccatcgga gtgattatat cttccaggcg ttgctccagc gcgttaaccg
     5341 taccgataac atcaaattcc tgacgcgaac aggtcgggca ggcgatgaag ttgatcccgc
     5401 gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat
     5461 cggccgccag cgatacgcgc agcgtgtcgc cgatgccttc agacagcagc agacctaaac
     5521 caatagcgga ttttactgcc ccgctgcgcg caccaccggc ttcggtgatc cccagatgca
     5581 gcggctgatc gatttgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag
     5641 acgctttcac actgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac
     5701 gcatggcgga ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt
     5761 tttccaacga tccggcgtta acgccaatac ggatcggaat gtttttatcg cgcgcacagt
     5821 caaccaccat gcgaatacgc tcttcattac cgatattgcc agggttaata cgcagacaat
     5881 cgacgccgta ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca
     5941 gcggcacgtt aacctgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta
     6001 cggagacacg gacgatatca gcgccaacgc gttccagcgc tttgatttga ttgaccgttg
     6061 cttcgacgtc tgtcgtacgc gtattggtca tggactgtac ggcgatggga gcaccatcgc
     6121 caatcggcac attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt
     6181 tatgcatgaa aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt
     6241 gagggtcaga cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc
     6301 ttgatactgg atctgtactg cggctggcgc accaattttc agtttgtacg gtgcctggcc
     6361 ggttaagttc aaattaccgt ctttacgctg cataccgcta aacagttttt taccggtagc
     6421 atcggtgact tccagccagc aatcggaagt aaagttcatc accagcgcat tcgggtcaac
     6481 cgccggcgtg gtcacgccag cctgatcggt tggcaacggc gcagcgccat ctggtgttgt
     6541 tgttgccgct ggtgccggag tcgcagcggt atcaacattt gcctgcgaag gtggaacaac
     6601 cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt
     6661 ggttgcggtg gtatccacag gtgatgtcgg cgtggtcgcc atagcctgat ctgtggttgt
     6721 cgacgtatct aacggtacac tctggctttg attattattc agttccgcag aagattgatc
     6781 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
     6841 cagaccaata accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcacgttt
     6901 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg
     6961 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
     7021 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
     7081 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gttgactaag
     7141 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
     7201 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
     7261 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
     7321 taagaccgtc tgtgtgctcg tgacaaagcc cacattcgct aaggctaact tactgttgca
     7381 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
     7441 aacttaatac ttcattcata aaagattacg ccacggtaca taaagtaacc gtggcgtaat
     7501 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
     7561 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcaat gtcatcacca
     7621 cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
     7681 atacggctgt tcgaactgcg tccatacggc gcgcccggga acgggttcca cgggatcagg
     7741 ttgatcttac acggcgtatc tttcagcagt tctgccagtt ggtgcgcgtg ttcagtgccg
     7801 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
     7861 ttctccagat aacggcgcac cgccgcaagg aacgtttcga tattgtactt tttgttgatc
     7921 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaacg
     7981 tcgatcatat cgccgagttt atccagcgcc ggaaccacac cggaagtgga aagcgtgacg
     8041 cgacgttttg acaggccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
     8101 ttgttcaggt tgagcagcgg ctcgcccata cccatcatca ctacgttagt gattgggcgc
     8161 tgaccggtga cttttgctgc gccgacaatt ttcgccgcac gccacacctg gccgataatt
     8221 tccgataccc gcaggttgcg attaaagccc tgctgggcgg tggaacagaa tttacactcc
     8281 agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac
     8341 accgtttcaa cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcagatgaa
     8401 cgctgttctt caaccacttc cggtgcacgg atttccgcca cctctttcag tttgccgcgc
     8461 aacactttat tgatgtcggt catctcatca aagttgtcgc agcaatagtg atacatccac
     8521 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
     8581 tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac gttttcaggt
     8641 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
     8701 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
     8761 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
     8821 gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg
     8881 aatcagaacc gtgggtacca ttttcggtca ggctgtcagc gtaatcagca cgcagagtac
     8941 cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacagcat
     9001 tttcaccttc cagcacggaa acaacgatcg ggccagaagt catgaattca accagaccat
     9061 caaagaacgg ttttccatcg tgttcagcat aaaagccacg agcctgttca acggtcaggt
     9121 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatactac
     9181 caatcacgtt ttttgctact gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca
     9241 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
     9301 ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa aataaaccca
     9361 gtttaagcaa caatcaggga caaaacagac tattgcatga caaatttcac tgtcgctatt
     9421 tgccccacgt catccatcac cagcaattga taatcgcctt tatccgtcaa atgcagggtc
     9481 acgttgcgcc cacgttcagt taacggttcg ccgttcagaa accaccagcg ttcacctgcc
     9541 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
     9601 gcgccatcgc gcacacctgt tagctgcagc gggagttgtg catcgtgacc gtatggcggg
     9661 caaattgtcg aggccggagg caagcgcaca gcgcgacgtt ctgatgcggg cagccagggt
     9721 tccagcggta gcggccagac atttatcatt tcttgtcgcg cctgagggca atcagcggca
     9781 acacgtttgc cgttttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc
     9841 tccggcagta atagagttgg cggctggctc ccgtccagca gccaggttgc caggcggcgg
     9901 cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga
     9961 cttacagagt caggacgtgg gtcttccggc aaattcacac tgcgcgacag taagatatta
    10021 ttaacctgat tcaacagcgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    10081 ccgtccggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    10141 gcatcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    10201 gcaccatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgcagcc
    10261 cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg caatttgcct
    10321 gccttgccgt ggcgagcaaa cgcggtatac gctgctgcca tatcttccag ttttgcgcca
    10381 gcgccgccga gaatgagcga aagattcggc gcagcaccgt tgggcagata caacggcaat
    10441 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca
    10501 gcaggtaagt tcagcgatcg caccagcgcc tcactcatgc tgatcgggcc atgaaaaccg
    10561 ctgtcaaagt tgccaggtcg gtaatcaccg gtgcgtcggg ggacgtcttg caacagcgat
    10621 gccgggtgga tcacgccttc atccagcgcc agaccataag caaacggttt gagcaccgat
    10681 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    10741 tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    10801 agtgagctgc gcggtggcaa tcgtcctttc cagttttgcg ccagttcttc cagacgtcgt
    10861 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    10921 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    10981 cttgactctt ttacccgctc acgggaccac acaccttgca cggccatccg ttcgagcact
    11041 ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct
    11101 tgcggcaaca ccgccagcat tgccgcctcg gaatagctta aattcgcagg cgatttcccg
    11161 agataagccc aacttgccgc accaatcccc tgcaacgtac cgccaaacgg ggcacggtta
    11221 agatacaagg tcagaatttc acgtttagac agatgccatt ccagttgcaa cgcgcgccag
    11281 agctgacgaa ttttgccgcc aaacgttttg gggtgaggat caagcaggcg cgccacctgc
    11341 atggtgagcg tgctgccgcc ggaaatgacc cgacccgacg tgagatcttg ccaggctgca
    11401 cgcgccaccg agaacggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    11461 atcagtgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg
    11521 ccgtcagcat cggcgaaacg ccagagtggc gtaccatcct gcgccaccac cacgcgtgca
    11581 ggattcactt cttgcaatgg cagaggccag agtttatctg ctgcccatgc tgccagaatg
    11641 atgataaaag gcgcggctgt cagcattatc cagcagccgc gtttggttaa caggcgaggc
    11701 atttacggtc tgactatcaa cagatcgtca gccgcaccag tcgcccgcca ttgaggaaca
    11761 tacattgatt ccaccatagg ctgcggcacc tgatacgttc ccggcgtcac cgcccgcgcc
    11821 agatacacca gcgttaccgg ttgatattca tcaacggcaa ccgccgccac aaagcgatcg
    11881 tcacggaact ctatgtgctt aatgctcgcc tgctgcatct ggttcagcaa gttctgcact
    11941 tcaccaccac tttgctccag gctggcgccg ccgttcgcca gattctggtt ttccagttcc
    12001 agccccgcag gcaacagatc cacgactaac gcatccggta cactgttact ggctttaacc
    12061 tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttaccg
    12121 tcagtaccca gaatatgacg ctcaatttgc agcacattgc tcgccggtaa aggtgcggat
    12181 tgcggataac cgctggcatc cacacgcagc cataacggct gatcgccact gttggtcacc
    12241 tgcaaggtgg caagttgatc gctattcaga ttgctggttt gcgctttctc gcctgtcagc
    12301 ggctcagcag agaaagtggt ttgcgcctgc catttgcccg gtaaatcctg aagagtacgg
    12361 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc gaacgcctgc
    12421 tgcgaaagtg tgttcaacag ggagtactgc tcatcgggta gcagtttgtt ctcttccagc
    12481 aatgagagca tcaacgcgct gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    12541 tcatcactat tacgcggcgt tttcagcgcc agcacaatcg cttcttcacc acgcgttgca
    12601 tcacccatga ttttcagcgc aacgccaagt tgcagcagcg gtaaaccaga agcggcatct
    12661 gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccaac
    12721 accagcgcag cgtaagactg tacggcgaat ttactggctt tgagattatc agcgtacggg
    12781 atcgatatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    12841 gcgtctgtcg gtacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    12901 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    12961 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    13021 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcat tggtataaag tgacgggaac
    13081 aggccgctgg cggtttgctc gagacagccg tacggatacg cttttaactc tttgatataa
    13141 cgtgcgatgt tcagcggtgg tttgccgctc aacaataact gcccttccag cgtaacaggc
    13201 gagaagtttt gcaatccatc agccgggagc gcccaggttt caccaggctg taacgccgta
    13261 ccgtaattga ccgtttgtgc cgggaatgcc ggacggacgc cgattttcca ctgcttatgc
    13321 tgatcggcaa cggtttcccc cggtaacgct aacccgctaa tggtggcctg aatgtcacca
    13381 tcgccataac ccgtcagagc acgcaccggg ataaacagcg tagtacgcac ccctggcgct
    13441 aattcaacag gtgcggggga attactcacc agttcaagca agccactggc ggtcagagca
    13501 acattaagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag gcgcgaggta
    13561 tcgccactcg ccataaagcg cggcatgtta agttcagcaa tcaccggtgc ggcaacaatc
    13621 actttgcttt cgttgcttcc gaagtcatct gccgtccagg cttgcgccat gacgcgcaat
    13681 tcaccgttaa aatcgccaat tggcagtgta accgagcctt cgccctgttc attgagcgtt
    13741 accggcagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt
    13801 ttcagctcat caccatcacc accaaaacgc agagctgcca gacgcccctg accttcaata
    13861 acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcctgc
    13921 cacggatctg gcgtgacgta atcagtaata ttcagcacac cgctatcaac ggcagacacc
    13981 agcacattca cctgttttgg catctcacca tttttattgc tggctttaat tttcacggtt
    14041 aatggctgat tggggcgcat tttggtgggg gtttccagcg ccagatcgag gcgacggttt
    14101 tcatcaccaa gcggcagatg caacacccca accgcgcgtt ttggcgtcgc ggagcgagat
    14161 ttatcgccag gacgtaccac cagcgtactt aaatacagat catgacgatt ccaggtttta
    14221 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacaac
    14281 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcaatatgc
    14341 aacttaatgg tatcgccagg gcgataactg gctttatcca gtttcagcgt gacacggtcg
    14401 ggtcgcactg caccaccccc gtcgctgtta tcctgccagc tataaccagc ccagaaacgc
    14461 acactactga ccgcttcatt cggcgcttta acttccagac gataagcgcc ccactctacc
    14521 gggaaactga ccttaccggt ttcgtccgct ttcagatcca gagtttgttc attttcgatc
    14581 agatcttttt gatcaaactg tgactgccag ccttcatatt ccgaccagtt ccagtagtaa
    14641 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgc
    14701 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    14761 accgtgctgt cggtgcgata atcgtaaacc gatttcgagg cgaactgcgg acggatcccc
    14821 ggcaatgcat cggcaggcca gatagcctgc tcagcacggc gcgtcaccgg gcgaccgccc
    14881 gattccagca aactaccctg gaaaataacc tgtaatggag aatgtgtttc cttccactgg
    14941 ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc
    15001 gtgcgggaaa gattttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca
    15061 gcttcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt cgcaagtgca
    15121 ccatacaggt agtaccccac cacggagaat ttcacttcat ctttcggcgt tagcggggtt
    15181 ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac gtggaaatcc
    15241 cacatccgat actggttatc gcccgtgtta gcgcgaatat gccacatacc ggttgccgca
    15301 ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgctc
    15361 ctgagtacct gcccgtcggg tttaatcacg tctaacttga tgggttgatc gggcaatgct
    15421 ttaccgtctg catcacgcag caaaccattg aggatgaccg tttcacccgg gcgataaaga
    15481 tcgcgcgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    15541 tccgctaagt ccagcgccgg gagttttaaa tcgagcagcg ttgtctgacc gtttttacgc
    15601 gccagcagta atgccgcgtt tttatcattt tccagttgca catgcccctg agcgtcactg
    15661 gttgcctgag tcagagtctg ccctttctca tttaataaag agacttcaat tccttgcagg
    15721 gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    15781 gaaacgccaa tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    15841 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    15901 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    15961 accagatccg ccatctgcag cagtttatct gactgccagt tcgccagcga attgcggtat
    16021 tcccactggc taatgaaagc gggcagtgat tctggtttca cacggaagaa gttaacatcg
    16081 acattgttaa cgttaagcgc cattaccggc agcccttcaa cgaccttacc aggaagcaac
    16141 gaaccacgac tggcaaaacc gacgctgggt tggatatcgc gggtagttat agttttttcg
    16201 tcatctttac taaaggttgc gttattgagc gctttgacct ccttgccaat agtgacgatc
    16261 aaatcacgtt tcggttcgag gtggcgtaaa cgcagctctt taagattatc tgacagctcc
    16321 caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg tgagaaatcc
    16381 tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcgcc atcgagttga
    16441 acttcagaga gatccagcaa tgtcagcgcc ttaccggcac tctgttgggc cagtttttgt
    16501 cgctccggcg cggaaagttt tgctgaactc acgttttctg aagaggccgc tttagtaact
    16561 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    16621 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac
    16681 aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca
    16741 gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc
    16801 ctcttttgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat
    16861 ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga
    16921 tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg aagatcgtaa
    16981 cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg atatcgaagc
    17041 gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt
    17101 ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa
    17161 tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc
    17221 gattctgggt ggtggacttg caggctggct gcgtgatgat ctgctattag aagaaggcgc
    17281 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg tggtgaaagt
    17341 aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc
    17401 tacacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
    17461 cggtgcattg aacgttccgt ggacggaact ggtacgtgaa ggcgaactaa aaacgaccga
    17521 tgaactggat gcgatatttt ttggtcgcgg tgtcagctat gacaaaccaa ttatcgtcag
    17581 ctgcggttct ggtgtaacag cagccgtggt tttgttggca cttgcgacgt tggatgtgcc
    17641 aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcacgggcag atttaccggt
    17701 tgagcccgta aaataagctt tttacgggca ataaaaaacc gccgaatttg gcggtttttt
    17761 attgctggtc tggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg
    17821 tctcagcgga cacaatactc gccagtaact ctctttttgt caagtaaaag agagttatta
    17881 ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg
    17941 cttaaggaaa ggatgttccg cgaccgttag ggcaagcgcg tctcagcgct tgctatcgcg
    18001 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
    18061 cggtgctttt gtatcgtact tattgtttct ggtgcgctgt taaccgaggt aaataataac
    18121 cgaagtctct tcggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc
    18181 cgaatgccgc cctttggggc ggcatatttt agattatccg gttctgttta aagtcacgca
    18241 aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa
    18301 ttcctttttc cccttttttc acctgacaaa tatcgatggg ttcatcacca ggtaatgtat
    18361 cggtcgctac acttcccgcc acctgaataa tttcttcgat atcaccatcg gcttcaatgc
    18421 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
    18481 tcaccggctt aatggtttta aataaggtgg taagtgaatc aatcatttgc gctggcggct
    18541 ctgcgacttc cgataatatc agcgattcac cgccttccag tacttcctgg ctactcagcg
    18601 gattcccttc ttcaccaatc aacaaactga tttcacgcgg cataaactct ttaccggttg
    18661 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
    18721 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa
    18781 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat
    18841 cttcaactac tgcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
    18901 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
    18961 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggagcaag
    19021 tgcctgatgc gccacgctta tcaggcctac aagggagtcg caatctattg gattttcgca
    19081 atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca
    19141 gattagcaat cgtgcgcaca ccaagtcccg tagcacccgc agaccactgt tcaaccggcg
    19201 ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa
    19261 agtgcgacag gaagcccgca gccgtacttg cacctgccgg atacgccgcg cttccggtat
    19321 tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac
    19381 gccagaacgg ttcgttctct tgtgaggcac tcgccagcaa acgaccggca agcgcatcgt
    19441 caaaactgaa caacgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg
    19501 tcgccgcatc aatgatcagt tccggtttct gcgcactggc atcaatcagg ccatcggcca
    19561 gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg
    19621 tgatgatatc gcccagcttg aacgcattgc cgctgatcag gttatccgca caacagagga
    19681 acagcttcac gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc ccggtaaccg
    19741 ttgccgcacc gcccatatcc gacttcatcg agtccataaa cgcggtctgt ttgatgctgt
    19801 agccgccgga atcaaaagtg atgcctttac caaccaggca cgcgtacact ggcgcttctt
    19861 tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgcc
    19921 cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccggt
    19981 aagtcacacg atcccctgcg acgttgcaga tcagatcaac agcacgctgt gccagttgcg
    20041 atggtcccag ctcttctgcc ggtgcgttga tggtgtcacg cacccagtca atgatcatca
    20101 ggcggttatc cagttcctga cgctgggcat cgtccagctc tggccactcc actttacgtg
    20161 tgcctttcgg ggctttgtaa ccttgccaga atgcccagca gcgatccgca tcccagcctt
    20221 caccgcttaa ctgaacatgc ttaatgccca gaccatcaac cttgcgcgcc gcacgctgga
    20281 tcaaccccag atcgtctgcc ccgttcaaat gcagggtaat gccatcatta ttaatgctgt
    20341 aagttgcttt ttctccccag cgcacgtcgg caggttgggt agaaagggta attttcatcg
    20401 cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc cccaggcagc
    20461 ccgttcattc actcggcttc gtccagccag accaacaaaa tcgcttcgag gattttctcg
    20521 ttcgatgcct gcgggtcgtc gtcgaaatct tccagagcgc aaatccactg atgcatatcg
    20581 gtgaatcgaa ccgttttcgg atcaatatcg ggatacgcat cgtacagtgc ttcgccaatt
    20641 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    20701 tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtaacgcgc gcctggcagc
    20761 ttaaacggct ttccggctcc agcccccagg ctttgtccag catgtcgtct tcctgctctg
    20821 agctttccgg cagcgagtca aaaccttcgc gaacgatgca gtggcaggtg gtgcaagcac
    20881 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagcgcaaca tcgagaatgg
    20941 tttcaccgct attcgcttcc agaacagcgc catcagggca gagatcctga tgaggcaaaa
    21001 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    21061 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    21121 tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    21181 tcgataacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcaccgtgc
    21241 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    21301 tcctgctcgg catagctcat tgagtctttg atcatcgagg cgatttcgct atcggtcagg
    21361 ccataagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    21421 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc
    21481 ggtagcgccg gaatacctcg cagcgcaaaa cgcgccagcg agcggcagtc ctgcaccagt
    21541 tcgcgctcac cctgcatcac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    21601 aaatcctggg cgcgggccac cggaatcgtg gtattacgcg ggatcacttt ctccaccagg
    21661 ccgcccatcg tttcgaggcc cagcgacagc gggatcacat ccagcaacag catttcgctg
    21721 tccggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    21781 tccgggtcga tagaagtcag cggtggacga ccgaaaaatt cgcctactcg ttcacgcacc
    21841 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcatc agcttctacg
    21901 cccgcatctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    21961 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcaca
    22021 gagtccgcat cgctcagcgc gattttggcc gcaatggcag catcgagcag ttcacgctga
    22081 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    22141 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg tcgccagcac ttcaaacacg
    22201 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc cgccgaggtc ataaacagcg
    22261 atcacgcctt cctggccgga atccagcccg taggcaatag ccgcagcggt cggttcatta
    22321 agtaagcgca gaacatgcag ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    22381 gcatcatcaa agtacgccgg aacggtgata acgacaccat ccagctcgcc tgccagggct
    22441 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    22501 agccccgccg ccgtttcaat catcggcagg ccattctcgc tggcctggaa ttgataaggc
    22561 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    22621 ctaatggtgt tggcggtgtc gagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    22681 ccttgctgtt gatagtgaac aacagacggc agcaggtgac ggccttcatg gtcagctaac
    22741 gtttcggcct gaccgctgcg cactgttgcc accagcgagt tggttgtgcc caggtcaata
    22801 ccggccgcca ggcgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    22861 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    22921 actgctccgc agtttatcga gaaaacgcag cttacgcaca gtatccgccg ccgcgtccca
    22981 cgtctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    23041 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagttc
    23101 ttcgcgcagt tccagctgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    23161 ggcgagatca aaaccgtgca aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    23221 cgtttgccag gcctggttaa tggtcgcgga ttgctgtacg gcggcgagtt gttccgcctg
    23281 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    23341 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
    23401 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    23461 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    23521 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttaag gccttctttg
    23581 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    23641 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    23701 taagccatac ctgaacaccc ggaggttctc acgcccagac gcaggccaaa ccctttaccg
    23761 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    23821 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    23881 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    23941 agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    24001 ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa aacgcgcgtc
    24061 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    24121 tgcacccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    24181 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    24241 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    24301 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    24361 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    24421 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    24481 acaggcggaa cctgaagaaa ccgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    24541 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    24601 gtaaacttct tcgatatctt tgatgccgtt ccacagacga ttacgcaggc cgcgcagacg
    24661 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca taccgacgat
    24721 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc catgcatttg
    24781 tgcttcgata cgtacgcgcg gtttacgacg cacatacagc gcaccaatac ctttcgggcc
    24841 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca gatcgatcgg
    24901 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    24961 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    25021 aacaaggatg gtgtcgtcac gcatcgctgc ttcgagttct ttcaggtcga taataccgtt
    25081 acgctgcggt gccaggtaag tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    25141 cagtaccgct ttatgttcgg ttttactggt gatgatgtgc ttgccttttt tctgataaaa
    25201 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    25261 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    25321 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    25381 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    25441 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    25501 accaggctac cggctggatg tacgaccgtg tttacggagt gtttagcact ccggcctgat
    25561 tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg
    25621 tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag
    25681 ttcgcctaaa gtaatgttat tgagaaaacc ggtaagacgg tcgctcaaat cacgccacag
    25741 cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc
    25801 atctacagat tcgtcaacgg cgctaatcac ctcgccaacg gcgatgctgc tggcatcttt
    25861 gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag
    25921 acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc
    25981 caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata
    26041 gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt
    26101 tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa
    26161 ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt
    26221 atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga cctgggattc
    26281 cgtgcactta caggcctgat aagacgcgtt aagcatcgca tcaggcaacc actgttattc
    26341 ggccttgtta cctttattct gctgctcaat agaagccaga atcccgcgca ggatattcaa
    26401 ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca tcacctgtcc
    26461 cggatggttt tcacggataa aaccggttgc cagcagcgtt tgctccagat gaccataaaa
    26521 acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct gctcgccatt
    26581 ttcctgagtt gccagccagg ccatgcgcac ttcataggca ataacctgaa ccgccatcgc
    26641 caggttcagc gaactgtatt ccgggttagc cgcaattgcg acatgataat ggcatttctg
    26701 caactcttca ttggtcaagc cgacgcgctc gcgaccaaac accagcgcca ccggggtatt
    26761 tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag
    26821 cgtgcgggag cgagcgctgg tgcccaccac cagactacaa ccggctaacg cttcgtcgag
    26881 cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca gggcaatcgc
    26941 ctgggagtca ggtttcacta gtggattaac cagccacaga ttggttaatc ccattgtttt
    27001 catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg
    27061 aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt atcataaaac
    27121 gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca
    27181 gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg
    27241 taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag
    27301 taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg
    27361 taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga
    27421 tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta tcaaacgtct
    27481 gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt
    27541 atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa
    27601 cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtactattc tggcgaccgg
    27661 cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg gcaaactgtt
    27721 taacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc
    27781 tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgc ccgtgggatt tcgcggcagg
    27841 cgagctgctg gttcgtgaag caggcggcat cgtcagcgac ttcaccggtg gtcataacta
    27901 tatgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt gttaaagcca tgctggcgaa
    27961 catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg tgatatcact
    28021 cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt tactttcttt
    28081 cgtcactctc ccaccatctt tcgccgatta atggatgata aagaagtcgc aaggaaaggt
    28141 tatggcgctg ccagtgaaca aacgcgttct caaaattctg tttattctct ttgttgttgc
    28201 cttctgcgtt tatttagtgc cgcgcgtcgc catcaacttc ttctattatc ctgacgataa
    28261 aatatacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta aggacggtac
    28321 tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg ccatcgcaac
    28381 catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc tggtcagttg
    28441 gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa
    28501 aggcacgccg tccctggccg gattgctgga cgatacgcaa agtgccatca atgtggtgcg
    28561 ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta ttggcggggc
    28621 gaatattctg gatgttattg gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga
    28681 ctccaccttt gcctcttatg cgaccattgc caaccagatg atccccggca gtggctactt
    28741 acttgatgag agttacagcg gcgaaaatta catcgccagc gtcagcccga tcccgctttt
    28801 actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag
    28861 cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt
    28921 ttccgatcgt cacggcgatg tttatcgcga acagatggtg gattttatcc ttagcgcatt
    28981 gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag
    29041 acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga tgcttgccaa
    29101 catttctgat gattagcatt cccttcgcca tttccctgag caaactttag ctattcttat
    29161 caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt
    29221 cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg ttgaccgcca
    29281 ccacggcaac tttcagcgag cttaatggtg tggatgatga tatcgccagt cttgatatca
    29341 gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact ggttatgacg
    29401 gcagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac
    29461 gacgtggttt ccgtctgtgt ccgtcattta ttaccagccg attcaccccc gccctgaaga
    29521 gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt
    29581 tagtgaatct ctgctcccga cggctgcaaa aacgctttga aacgcgcgat attcatttcc
    29641 tgtgtctgta tttgcaatat tgtttgttgc aacatcacgc tggaattacg cctcagttta
    29701 atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg caggaaattg
    29761 gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca ctatttatgg
    29821 cactactttt ttccatgttg cgggttcccg atccactgcg ggatgcgcat cagcgggaca
    29881 gacaactgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc
    29941 gtttttatga tgaacagggg ttatgcgatc agctttatac ccacctcgcc caggcgttaa
    30001 atcgcagttt gtttgccatc ggtattgata acaccctgcc ggaagagttc gcccgcctgt
    30061 atccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt gaatacggcg
    30121 tccatctttc tgatgaggaa agcggtctgg tcgcggtgat ttttggtgcc tggctaatgc
    30181 aggaaaacga cctgcatgag aaacagatta ttctactgac cgggaatgat agcgagcgag
    30241 aagcgcagat tgagcagcag ctacgcgaac taacgttact gccgctcaac attaaacata
    30301 tgtcggtaaa ggcatttttg cagacaggcg ctccgcgcgg cgcggcactg attattgcgc
    30361 cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac ctgacgttga
    30421 caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc
    30481 gggcgcaaaa acattgccgg aagcgccacc agcgccatca cccagaacac gccgtggccg
    30541 agatattgat acaggaaacc ggcgaaaacg gtcatgatgg cgatactgcc gcccatcgcg
    30601 acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc agcaatatag
    30661 cgcatggcag ccaggtggca gaccgtgaag gtaccgcaat gcagaatttg caccactatc
    30721 aaccacggca acgccgtcgt tgctcccata atgccccagc gcactacgcc gcaaatcgcc
    30781 gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagcgcaaaa
    30841 ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga ggccgagtag
    30901 ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat
    30961 aaacaaacgc aggccagaaa gcgccagttc tggcgaacca acgccaacca cgcagaccag
    31021 ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag
    31081 ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg ataatcaaac
    31141 atactgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga gccccacagt
    31201 ctcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc
    31261 aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat tagccacgct
    31321 acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc
    31381 aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa actcccgagg
    31441 aaacgggcaa ccagacccgc ccccaataac aggccgatgg tttctggcgt taaaccaatc
    31501 cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa
    31561 tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg
    31621 ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct
    31681 tcctgacggt tatcctgcat atcaggaaat gacaaaaaag tgcggcccac aacacaacat
    31741 gtaaaaaaga acgatgaaac aggtgaaaat cgcaatttat tgaactgaca aactcatgtg
    31801 agacggataa ggcgtgcatg tcgcattcgg caggaaacaa taagcacatt gtcagccatc
    31861 tgcgttatgc cgttatacca gaaacgaata tctcaacgcc tggggcgtgg cgaggtacca
    31921 gagtgtgtat tacgtttaaa tcacatcatc ttgcaaaggg attggttatg aactcactac
    31981 gttattttga ttttggcact gcccgccccg ttttattatt aattgcccgt atcgccgtgg
    32041 tcttaatttt cattattttt ggttttccca aaatgatggg ctttgacggt acggtccaat
    32101 atatggcctc gctgggcgcg ccaatgccga tgctggcagc gattattgcg gtagttatgg
    32161 aagtgcccgc cgcgatatta atcgtgcttg gctttttcac ccgtccgctg gcggtgctgt
    32221 ttattttcta cacgctgggt acggcggtga ttggtcacca ttactgggat atgaccggcg
    32281 atgcggttgg gccaaatatg attaatttct ggaagaatgt cagtatcgct ggcgcattcc
    32341 tgctgttggc aattaccggg ccgggggcga tttctctcga tcgccgttag gaaagatgcc
    32401 ggatgtggcg tgaacgcctt atccggcatt ttataaatta cagcaacgca acccgcaacg
    32461 acatctctga aattgttgat tcccctggtg caagcgcaat gaggtcacca ccttccgggc
    32521 gatgatggtc atccggcgca tgactcatcg gttccagaca gaagaaatca aacgcatatc
    32581 ctttatcaaa cgcagggtct gaaacaaaga tgaaataaca cggtgcaggt ggcgtcgttt
    32641 ccatgatgat ggcatacccc tcctgcggct gctcgatgcg ggccttacca ttccatccgg
    32701 caaaaccatt gtttacccac tggcgcggca acggcgcgag ctggttaaaa tccagctcct
    32761 gcggtagctg ctcgcaaaac tcacccgcca gccactgctc ccgctccagc cagtaaccgc
    32821 tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa ataaggatgc cagccggtac
    32881 caaatggcag cgtctctgcc ccttgattgg tgacagcgag cgtcaccgtc agcgtatccg
    32941 ccgttaaatg aaatgcctgg cttacccgat agtgataaac accgctgaga tgttcataca
    33001 ccagacacaa gctatcatcg ctgcgcgaga cacattgcca ctcgcccaac cagccatcgc
    33061 cgtgtagata gtgcgcatcc cactcaacat tcggttgcag ttgatactca cgcccctgcc
    33121 agacaaaacg gttgccgctc acccggtttg caaatggtac cagcggaaaa caagaggcat
    33181 cggttgccac accgcttttt ttaccaggac gtagcagcgg cgtcgtatcg cgccagaagc
    33241 cttcgattac gccgccctga tcagaaacgt ccagctttag cggcccgtga gataaggtat
    33301 agatggtcat ccgcagactc cttaatccgg gaaattaatc acaactttcc cacatttgcc
    33361 gcttgccatc agtgcataag catctcctgc ctgttccagc gagaaacgat gggtaatggc
    33421 gttacgcggc cacagtttcc agtccgttaa atcgtgggcg catttttcca tatggaacaa
    33481 actggtcacc caggagccaa taatccgccg ttgatggtgc atcaggtcag cgctaacctc
    33541 gaactcgact tttccggttt caccaatgta aaccacgcgt ccccagtcag ctgtggattg
    33601 cagtgccagt aagcgacctg cggcattacc ggaacaatca agcgcaacat ccgcgccgcc
    33661 gtgagtgagt tcggcgatta tctgcggcag accttcggtg gtcgctaaat agccgtgatc
    33721 catcactcct aactgtttcg ccatcgccag acgttccggc agcatatcaa cgccgatgat
    33781 cctttttgca ccacgacctt tcgccagcat catcgccatc atgccgaccg gccccagacc
    33841 gaccaccagc acgttatcgc taccggaaac ttcgccgcgc aaaatccctt catacgctgt
    33901 accaacgccg caactgataa acgcaccatc ttcatagctc agcgcgtccg gcagcaggat
    33961 cagatctttt tcttccgcca gcaggtattc agcatggccg ccgtcacgct gccagccgta
    34021 agccgctttt ccttcgccag tacaagaaat aggaaaaccg cgacggcagt tcgggcaaaa
    34081 accacagcca gaaatgtgat acaccagcac gcggtcgccc tctttaaaat ggcggcagcc
    34141 ttgccccatc gccacaatct gcccgcacgg ttcatgaccg ttgataaatc cctggtataa
    34201 cggtttatcg ggtgccgccg ccgtagcgcg gtgctggtgg tagatatagt ggacatcgct
    34261 tccgcaaatc ccggaggatt tcattttgat cagtacctgg ttaatccccg gcgtcggcac
    34321 cgcaacttcc cgcagatcga cggtcgaatt tcctggtaaa taagctgcca gcatcgtttt
    34381 cataaatccc tcattaacaa tacgattaat tttcatccct gccgcacccg cgccaggggc
    34441 gttaaggttt agcgtttcgc tttactacta cgctgggtca gcaagatatt tgccagcacc
    34501 gccaccacaa tgatgacgcc gcgcaccacc tgctggaaaa aggagttaat cccgagcagc
    34561 accagaccgt taccgattag cgtaatcacc agtacgccaa gcagcgtacc gaacaacgag
    34621 ccgcgaccgc cggaaagcgc cgtaccgccg accacgaccg cggcgatgac gtcaaactcc
    34681 agaccgtttg cggcacctgc gttaccggaa ccgaggcgcg ccgccaacaa aatgccggtc
    34741 accgccgcta ataatcccga aagggtaaaa ataagaatgc gtacccgacg aacgttaata
    34801 ccgcacaact gcgctgcagt ggcattaccg ccaacagcaa aaaccgagcg cccgaaggcg
    34861 gttttgcggc taatgaacac aaacagcgca aataacacca tcatgatcag cgcggatacc
    34921 ggcacaccga gaaattgtcc gcccagccag tccagcactt cgttttcgtt aattggcact
    34981 ggcaaggcgt tcgtcataaa cagccccatt ccgcgcaggg cgctccataa acccagcgtg
    35041 gcaacaaaac ttggcacgtt aaacacgccg cgcagcaccc cggcgagcgt tcccatcagc
    35101 gcacctaaca gcaacaccag cagacaagca atcgccagcg gaacttcaaa ttgcagcaaa
    35161 aacgccaggc acactgaaac aaaagccacc atcggcccaa cgctgacatc aatttcaccg
    35221 gagataataa tcagcgtcat cgcccaggcg gcgatcccga tggtggcggc atcgcgcagc
    35281 acgttcattt ggttattcaa tgagataaag ccaggcgcat tcagggagaa gaccagataa
    35341 agaatggcaa tcaccaccag caaaccgatc tcattaatat ggcgactgac aaattgtttg
    35401 agcgagacgc tcttgccctg cggcaatggt aatgacgaag cagacatctt caggttcctc
    35461 ggtaaaattc atcagtggac agaatggcgg acatcagctc atccacattg accggcgagt
    35521 gaaactcctg cgagaacgtg ccgtgctgta ataacaggat gcggtcacac accagcggca
    35581 actcctccac ttcactggag ataaacacca cgctttttcc ttcggcagcc agctcgcgga
    35641 caatacggta aatctgctgt ttggcttcga tatcgacgcc gcgcgttggc tcgtcgagca
    35701 acaaaatctg gctggcggca tagacccaac gaccgatcac cactttttgc tgattgccgc
    35761 cagaaagcgt gccgatgggc gtttcgctac tggcggcctt gaccgtcatc cgctgcatca
    35821 cctcttcggt caggcggcgg atggtggacc attgcagcac accgttggcg ctgatttttt
    35881 gccgattggt cagcactgta ttttcgtcaa cgcccaacca gggaatgatc cccgcttctt
    35941 tgcggttttc tggcgtatag ccaatgccgc gtttcagcat gtcgccgtaa tcggggcgca
    36001 tgattttctc gccgttgata acaatttcgc cctgttcata cgtctccaga ccaacaatcg
    36061 ccttcagcaa ttcactgcgc cctgccccca gcagaccagc aatgccgagc acttcgccac
    36121 gacgtagcgt aaaactgata tcctccagct tgggcttatg gcgtaacgca cggacttcca
    36181 gcacggcttg atccataatt tcctgagggg caaccggcgc aatatcaacg tgatcgcgcc
    36241 cgagcattag cgacacaata tgatgcgtag aggtgttttc gagcatcaca tcgcccgcca
    36301 cctgaccatc gcgcataacg gtggcacagg aggcaatgcg gcgaatttct tccatccggt
    36361 ggctgacata aatcaccgcc acgcccagtg ccgacatctt tttcaccgcg ctgatcacca
    36421 gttcaacttc cgcactggca agcgagctgg tgggttcatc aagaatgacc acgcgcggct
    36481 cgcccttcat cacccgcgca atttccacca gttgcttttg cgccgggctt agcgttgaaa
    36541 caagttgttc agggctaacg tcaacgccca gcgcctgtaa gcaacgttgg gcatcctgcg
    36601 ccatttgcag gtaatcaatc atgccgttgc ggcggggcca ctgaccgagg cagaggtttt
    36661 ccgccactgt cagcccttcc accagactta actcctgata aaccgcgcgt acccccagtt
    36721 cagcggcacg gcgagtcagc gtagcttcgt caccttccag tcgcgtctcg ccaatccaga
    36781 tatcaccgct atccggacgt tcgctaccgg taagcattcg aatgagggtc gattttcccg
    36841 caccgttttt gcctaacagc gcacgaactt cgcctttttt gagcgtgaag ttaacgttat
    36901 ccagcgcaac gactccggga taacgcttat ttcctgccac cacttttgct accgggactg
    36961 cctctgttgc cgtgaacata gggacctctg cgaatcagcg attagggcag accatcaacg
    37021 tgcgttgcca gccactgttt accgtcttcc gttttggtat agagatcgat tggcacctga
    37081 atcacttttt caccgtcggc ttgtttattg ataaccttca atgtttgcgt aaaaacagca
    37141 ttgcccattt tcttaccaga aatatccacc accgctttca gcacctggtt gttttccagc
    37201 tcctgagcaa tttcagtggt catatcagaa ccgaaaacag caatttttcc ggcctgattt
    37261 tgattacgta ccgcttttac cgcgccgagt gtcgctccgc ccgattcccc cataatggcg
    37321 ttgagatccg gcgtggagat aatcagtttt tcaccgacag aaatcgcttt atctaaaaca
    37381 gtcccttcct gattagcgac aatttgcgcg ccgggaacgc gggtttttaa tacttcttca
    37441 aaccctttac gacgttgcac acaaacttca aaggcttcgc aattgatgac ggcaattttc
    37501 ggctggtcaa ttttgttggc aataaaataa tcggcggcag cgttacccag ttttttacca
    37561 aattccagcg ggtcaccaac cagatatgcc gagacatatt tatcgacacc cttttgatta
    37621 atacaggtgt tgtagcaaat caccggaata cccgcttcac tggcgcgacg aacggtacgg
    37681 ctactgccat tttcagacac tgccgataaa ataatggcat cgacattacg cgccacgagg
    37741 gtatcaacaa aggtactttc tttcgaaata tcgccctgag cgttagtttc aattaactgt
    37801 acctgtactg aagaatcttt tgccgcatcc tgaacgccct gacgcactcc ggcgtaatat
    37861 ccctgggtat caaggtatat tgcgccaata gtcatttctt tatcagaagc cctggcaaat
    37921 agtgcgctac ccaatagtgt agccattaat aataaattac gggttcttgt cattttttta
    37981 ggcatagtgc ctcctgtagg gtttttattt acaacggctt attttaatta tttgagcgtg
    38041 agcggcagca cgtgccgctc aaatattaca gttaataggg tttagtgaac gtagctaaaa
    38101 ataaaaggca tcacggtggt gaataatgcc gctttcggcc aggctggatt aatcgccatc
    38161 aattcagcgc gagcagattc atagtgcgca acctccccta atcccgcggc cgccagcatc
    38221 gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag cgacaacaag
    38281 tcaggctgcg agacggcaaa gaaatccgcc tcgatactgg ttttcgccat ctcttgtgcc
    38341 cactgtttca tttcactaaa cagttgctgt gcagtttgtt gttcgcccag cagtcgcagc
    38401 gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact gtggatgtta
    38461 atggtgcgat cgccggtcgc cgccagacgt aaacaacgcg tcgcttcagt ttcatcgccc
    38521 tgcgcgttgg cgcatactgc ctgccagaac cagatgtcgt tgtcagtttg ccccggtaaa
    38581 cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc gctggcctgc
    38641 tgcggctctc tgtcatcaag atgctgccag gcgcgtaata actggttgag gataaactgg
    38701 ctggtgacct tcccttcccc accttcccac gggtggaatt tgcgcgtggc gagaatgtcc
    38761 gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc ggtcatgtcg
    38821 tcgcgtttca gcgcaatttc cagattatct tccagacgcg ccagtcgttt ctccggtgtg
    38881 acaccgctta acttatccag caaatcccgt tcgaaaagca gacgtgcatc ctgcggcgca
    38941 agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgctg cttattccac
    39001 gcatggatcg ccaacccgcg ccagccgtcg gcaaactccg gcgacatctc tacgcaacgc
    39061 tgccataagg caatggcttt gccgtagcta cgcttgttgt agtagaagca ggccagtaaa
    39121 tgccgggcaa accagcactc ttcgatactc tccagcgccg ccacttcttc cagcgtattc
    39181 gggaaacgga caaactgcgg gaaggcatca atagcttttg cgaccagttc tccgcgttca
    39241 gcttttggta gcaaactggc ttgcaggtaa agcggtagcg tgcgctgaca gtccagtgcg
    39301 ttcagcatat ctgccgccag ggcgggcatg ccccagttaa tcagttgccc ggcggtcatc
    39361 agggcgttaa cgtcgcgtcc ctgacaaaga cctcgccact gcgcgagggc tgattcgcta
    39421 cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc acgcagcagt
    39481 ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagtag caggttatgc
    39541 aggcaaagca cttcctgatt ggttgggcag gcgcgaagac tttgttggca aaaatccaga
    39601 ccagcgtcga agttaccatt acgcgccgcc agtcgtgcca gaccataata gccgcccgct
    39661 ttactgttgc cgctccagac cgcacgccag aaatcctctt cggcttgttg atattgcccc
    39721 tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc cgcactgcgg atttttgttc
    39781 agcgcatgtg cgcgtttcag tgcctgactg gcatatacca ccgcttgcgg gaaatctgca
    39841 cggttatatt ccagcattgc cagcgccaga ttacagcgat aatccagcgg atccagcgcc
    39901 acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg ctccagatgt
    39961 tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag tggcgctttg
    40021 gcgacgtccg gcaacggcaa cgcttgcggc tgatgttcat gataactcag tacaatattg
    40081 ccgtcggcat cggagagctc aatggtcagc ctttcaggat tgataccgtg caacacgccc
    40141 tggatggcgg tcgctggtgt cagggccacg gcatcatcga gcaacgcgtt gcatttgccg
    40201 atttcgcgga tcgccaggcg gtatccgttc aacggagaga tggcatacag cccccactca
    40261 atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt ttgcaccatg
    40321 cccagagaat gataaggcag gaaatactgc tcgaaacgct tctcctcgta agcatcaagc
    40381 caggtaaaat caggctggtt atcggcaaaa ataccggtca tcagttcgat atacgggccg
    40441 ttattgtcag tcaggctctt atcccacgcc tggccaaatt cactgtgtcc ccagctccac
    40501 tgttttttac ctggcgcaat atggtggttg gcaacgtgta gcaaaccacc atcttcatcg
    40561 tgacaccacg cgccaacaaa atcgtactgt gatttttcag ccatatatga ggttggaacg
    40621 ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt gtagtaagtg
    40681 ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac cgccgttaca
    40741 tccggcggga agacgctctg atgcccttcc ccccctttca ctgccgggtt ggcccaccac
    40801 aagaaatgac gcggcgtggc gttgccgtta tagacgcgac tggcgatttc cagcgccgcc
    40861 cggtcagggc gcagggtgaa acctgtcatc acctgtaaac cgtgcatcga ctccgtttcg
    40921 ccgacccaca ccgtctgtgc gccgtcgtca tgggcttcga gggtgaaatc aacgggcata
    40981 aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa tcccgccgga gatccacggc
    41041 cccagcagtc ccaccagcgc cggtttaatg acttcattgt gataaacaaa atcgcgctgt
    41101 ttcactttat cccacgcgcg atgcacccga ccgcccagtt ccggcaggat catcactttg
    41161 atgtagtcgt tttccagcca caccgcctgc caggatttca gggttttctg ctcgctcagc
    41221 gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac gcgattttcc
    41281 aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc aacgcgctct
    41341 tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac tgaattaaaa
    41401 gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc acgcaattaa
    41461 ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt cgccaccata
    41521 acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa tcaacgctgg
    41581 cccggctggc gcaaatttcg attccggcgg tcagtaatat tttgcaggaa ctggaaagcg
    41641 aaaaacgggt ggtgaatatc gacgatgaaa gtcaaacgcg cggacatagt agcggtacat
    41701 ggctgattgc gccggaaggt gactggacgc tgtgtctgaa cgtgacgccc accagcattg
    41761 agtgtcaggt cgctaatgcc tgtttaagtc cgaaaggcga atttgagtat ttacagattg
    41821 atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat cgccaccgta
    41881 aattgtggcc ggatcgcacc atcaatctgg ctctggcaat ccacggtcag gtggatccgg
    41941 tgaccggcgt gtcgcaaacc atgccgcaag cgccgtgggc aacaccgatt gaggtgaagt
    42001 acctgctgga agagaagctg ggtattcggg taatggtcga taatgactgc gtgatgctgg
    42061 cgctggcgga gaaatggcaa aataattcgc aagtgcgtga tttctgcgta atcaacgttg
    42121 attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc agtttgtatg
    42181 gcagtggaca gattggccac accatcgtta atccggatgg cgtcgtctgc gactgcgggc
    42241 gttatggctg cctggaaacc gtcgcctcgt taagcgcatt aaaaaaacag gcgcgggtat
    42301 ggctaaaatc acaaccggtt aatactcagc ttaatcctga aaaattgact accgcgcagt
    42361 taatcgctgc ctggcagagt ggagaaccgt ggttcaccag ttgggttgac cacagtgcca
    42421 acgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat cagatttggt
    42481 tgtacggtcg cagttgtgcc tttggtgaga actggcttaa taccattatt cgccagacgg
    42541 gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa agcgacacag attggctttg
    42601 ggcaattaag ccgcgcacaa caggtactgg gaattggcta tttgtatgtt gaggcgcagt
    42661 tacggcagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc cttttttcaa
    42721 cagattaacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc gagaacttta
    42781 cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc acacatccag
    42841 ccagccagtt ctttcgcttc ggcttcttta aagccacgac gggtaatcgc cggagtacct
    42901 acacgaatac cggaagtcac aaatgggctc ttcggatcgt tcggtacgct gtttttgttg
    42961 acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag gtttttatca
    43021 accagatcaa ccaggaacag gtggttatca gtaccgccgg aaaccacttt gtagccgcgt
    43081 tcgaggaaca cttctaccat cgctttggcg tttttcgcga cctgctgctg gtaagttttg
    43141 aactcaggtt ccatcgcttc tttcagagca accgctttac cggcgattac gtgcatcaac
    43201 ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag ctcttcgcta
    43261 ccacctttcg ccaggatcag gccgccgcgc ggacccgcca gggttttgtg agtggtggta
    43321 gttactacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac caggcccgca
    43381 acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt cagcgatttc acgcattttc
    43441 gcccagtcaa ccacgccgga atatgcagag aagccaccga taatcatttt cggtttgtgt
    43501 tctttagctt gtttttccag atcggcgtag tcgatatgac cagtagcatc gataccgtaa
    43561 ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt caggtgaccg
    43621 ccatgcgcca gattcatacc cagaacggta tcgcctggtt ccagcagcgc ggtgtagacc
    43681 gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc gccgaacagt
    43741 tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca accgccgtag
    43801 tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc ctgcgcctgc
    43861 attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc ttcctgacgt
    43921 actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat gttcatttca
    43981 cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc tttataggcg
    44041 gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca ggtcttgaca
    44101 aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa taaagagctt
    44161 gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt catcacattc
    44221 tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata agatgcattt
    44281 gagatacatc aattaagatg caaaaaaaag gaagaccata tgcttgacgc tcaaaccatc
    44341 gctacagtaa aagccaccat ccctttactg gtggaaacgg gaccaaagtt aaccgcccat
    44401 ttctacgacc gtatgtttac tcataaccca gaactcaaag aaatttttaa catgagtaac
    44461 cagcgtaatg gcgatcaacg tgaagccctg tttaacgctg ttgccgccta cgccagcaat
    44521 attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa tcgcgcagaa gcacaccagt
    44581 ttccagatca aaccggaaca gtacaacatc gtcggtgaac acctgttggc aacgctggac
    44641 gaaatgttca gcccggggca ggaagtgctg gacgcgtggg gtaaagccta tggcgtactg
    44701 gctaatgtat ttatcaatcg cgaggcggaa atctataacg aaaacgccag caaagccggt
    44761 ggctgggaag gtactcgcga tttccgcatt gtggctaaaa caccacgcag cgcacttatc
    44821 accagcttcg aactggagcc ggtcgacggt ggcgcagtgg cagaataccg tccggggcaa
    44881 tatctcggcg tctggctgaa gccggaaggt ttcccgcatc aggaaattcg tcagtactct
    44941 ttgactcgca aaccggatgg caaaggctat cgtattgcgg tgaaacgcga agagggtggg
    45001 caggtatcca actggctgca caatcacgcc aatgttggcg atgtcgtgaa actggtcgct
    45061 ccggcaggtg atttctttat ggctgtcgca gatgacacac cagtgacgtt aatctctgcc
    45121 ggtgtcggtc aaacgccaat gctggcaatg ctcgacacgc tggcaaaagc aggccacaca
    45181 gcacaagtga actggttcca tgcggcagaa aatggcgatg ttcacgcctt tgccgatgaa
    45241 gttaaggaac tgggtcaatc actgccacgc tttaccgcac acacctggta tcgtcagccg
    45301 agcgaagccg atcgcgctaa aggtcagttt gatagcgaag gtctgatgga tttgagcaaa
    45361 ctggaaggtg cgttcagcga tccgacaatg cagttctatc tctgcggccc ggttggcttc
    45421 atgcagtttg ccgcgaaaca gttagtggat ttgggcgtga aacaggaaaa cattcattac
    45481 gaatgctttg gcccgcataa ggtgctgtga tttgatgttg ccggatggac acatccggca
    45541 acccttgacg cgttttaaat tgccgcgtcg tcctcttcac cggtacggat gcgaatgacc
    45601 cgtgccacgt cgaagacgaa gattttacca tcaccgattt tgccggtttg cgccgtgcga
    45661 ataatggtat cgacacaggt atcgacaatg tcgtccggta cgacaatctc aattttcact
    45721 ttcggcagaa aatccaccat atactccgca ccacggtaca gctcggtatg gcctttctgg
    45781 cggccaaaac ctttcacctc agtgaccgtc atgccggtaa taccgacttc agccagtgct
    45841 tcgcggacat cgtccagctt gaagggttta ataatcgcat caatcttttt catgctattc
    45901 cttgaaaagg tcgcctgtct tttgatctgc taaacgtaac acataacgcc aattcattcc
    45961 ttgaaatcgt ttgcatccag ctcgtttcgg gaaagcagtt tataaaattc tgtccggttg
    46021 cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg tgatttgcag cagcttacgc
    46081 aaatagttga gttcaaactg attacgtgcc tcaacaaagg ttggcagcgc cgtattttca
    46141 ccctccagcg cctgctccac cagcgcatca ctaattaccg gagatgaggt cagcgccaca
    46201 cactgttcaa tcacgttgac caactggcgc acgttgcccg gccagcttgc agtcatcagg
    46261 cgtttcattg catcggtgga gaacgcgcgg acaaacggtt tatgtcgctc tgccgcctgg
    46321 cgcaacaggt gatttgccag tagcggaatg tcttctgtgc gctccgccag tgccggaatt
    46381 ttcaggctga caacattgag gcggtaataa agatcttcac ggaattcccc acgcgccatc
    46441 gcttttggca ggtcgcggtg agtggcggaa ataatccgca cgtcaatatc aatatcgcgg
    46501 ttactgccca gcgggcgcac tttacgctcc tgcaacacgc gcagcagttt gacctgcaac
    46561 ggtgcaggca tatcgccaat ctcatcaaga aatagcgtac cgccttccgc cgcctggaat
    46621 aaaccttcgc gattgctgac agcgccagta aacgcgccac gtgcatgacc aaacagctcc
    46681 gactccagca attgctcggg taatgcgcca cagttaatag caataaatgg cttgctgttg
    46741 cgcgggctgg cgttgtggat agcctgggcg aaaatctctt tcccggtgcc gctctggccg
    46801 ttaatcaaaa cgctaacgtc tgattgtgcc accagccgcg cctgttccag caagcgcagc
    46861 atcagcgggc tgcgggtgac aatcgcctcg cgccaacgtt cgtcggtggc tggcgcggat
    46921 tgttccagcg catcgtcaat tgcctgatat agcgcatctt tatcgacagg cttggtgaga
    46981 aaactgaaca cgccctgctg tgttgcagca acggcatcag gaatagaacc atgcgcggta
    47041 agaataatta ctggcattcc cggctgcact ttctggattt cagcaaacag ctgcataccg
    47101 tccatttcat ccatccgcag gtcgctgatg actaaatcta ctttttcgcg attcagtacc
    47161 cgcaatcctt cagcaccact ttccgccgtg accacactgt agccttcgct ggtcagacgc
    47221 aggccaagca gtttcagcaa tcccggatcg tcatcgacca gtaataaatg cgcaggttta
    47281 tggctcatca ggagtgacct catcggtgga tggcgcgggc ttttcactct cgtgcggcgt
    47341 atccggcgag aaatttccag ccggtttgcg ggtcgagagc tggcgttcaa tatcggtcag
    47401 gttttccagc ttgcgagtag tgagttccag ttgctgttgt agaacgtgat gttgctggcg
    47461 caaagtatcc agctcgctgt cgctggactg ctggagtttg ctgtagcgtt ggcgctcttc
    47521 cgccagttgc agttgcagcg cctgaccgtc gcgccaaagt tgatacagtg ggcgaacctg
    47581 tgccgggatc tcggtactta gcgcatcaat gcgcgcgacc agctggcggc gctcatacgg
    47641 cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag gtattttgcc agttgccgtc
    47701 gtcatattgg cgggcttgct gacgcgactg cgcaggcatt aaacgatcag cacaatccat
    47761 cgcccgcagc cagtaaagcg gattggtttc ggttgattta ccttgcagcg cccagatgtc
    47821 gctacattca gtagaaagat agtcagccag ttgataaacc ggaatttttt cttctgctgg
    47881 cgtatcaatg gctggcctat tgtgactctg cacgcaaccc agcaatgcca gacatggcag
    47941 ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa atgtgtcgca tattcaccag
    48001 acttaaagcc tatcccagtg ggcgtaattg ttgcaggcag tttggacatg gacagcgcgg
    48061 agaaaccgga gcgtacatgt cgtacgtgaa gatttcgagc actgcccggg gccgaactga
    48121 caaataaaat agcctgatgg gatgggctgt tagcattatt tcgtgttttt cgacgacggt
    48181 aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga ccagatacag ttccccttgc
    48241 atacggcgaa tacaatccct ggcaatgctt aatcccagac cgctgccctt caccgcccct
    48301 tttcgctggt ggcttccctg aaaaaagggt tcgaagatca tggcgcgttc ctcttgcgga
    48361 atgggcgtgc ctgtattgat gacatcaata taaacccgcg caccatgtaa actgctgcga
    48421 aggcaaatgt taccggattc agccccgtag tgcaccgcat tggagtaaag attatccagt
    48481 acgctcatca gcagcattgg ctccgccagg caagctgttg ctttgagatc aacgtcggta
    48541 tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa ccaatgtctc caccagcggt
    48601 gctaactcaa cattctccag ttccaccgca ctgtccgcct gtttacggtt gtaatcaagc
    48661 agttgttcga tcagtttttg caaattgcgg ctgctgctat caagaatgct caccacctct
    48721 ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca gtaattcagt gccctcgcgc
    48781 atactcgcca gtggcgtttt taattcatga gataaatgtc ttaaaaattg atggcgttgg
    48841 gattccagcc atgacaggcg ctcacttaac caaagaatac gttgcccaac cgagcgtaac
    48901 tcgctcggtc cactgaacga gacgctattg cccagagaac gcccttcccc cagccggttg
    48961 atcatgcgct cgatattttt caccggaccg ataatcatcc tcgtgaaaag cagtaccatc
    49021 accagactca ccagaaatag caccagcgat tgccaaccaa aatattgccc acgttcggcg
    49081 atttcacgct gaagttgctg cccacgagag aacaccactg tgcgcgtggc ctgcaccatt
    49141 tcggtattgg cactggcaaa ggcttccaga cgcgcggcgg cagcagcatc gggaccgctg
    49201 ttgttacact gaagttgagc cagattgttc aaatcctgac gtaatgcctg gtagagttta
    49261 tcgtccggca gcacgcctgc gtgggcatcg agcatttcgc tgtaacgctt gcgctggctt
    49321 tgataaacct tcgcaagcgt agggtcgtcc agcacgcagt actgacggta gctgcgctcc
    49381 atctccagtg ccgcgttggt catcgcttca ctgcgccggg catcaataag cgtagtgcgg
    49441 ttaaccagcg ccgcctgatc gctaagtgca ttcaggcttt gccaggcttg ccatgccagc
    49501 accaacaggg gcagcagaat cagcaaaaat gccagcatta ccagttgtcg taatgagcgg
    49561 ggaaaaacgg gccagcgttt caaggcgtta ctctcgtcag acgcgaatag cctgatgcta
    49621 accgagggga agttcagata caacaaagcc gggaattacc cggctttgtt atggaataag
    49681 gcggtgccta actcgacgtt tcgcccgatg gttgatatag ctaagctgat atcagaagtt
    49741 ggacggcagg caccttgttg tgcgtcattc gaattttatg tagcacgtcc cgaaggggct
    49801 gacataagtc ggtgaatgag ccactgctta ctattatgca gtaactgtgc caataaagaa
    49861 aatagtttgg taacatactg attatacgct gctttgaggg gtttatggct tctccgctat
    49921 ttgaatgtaa atcagcc
//
LOCUS       DAJOTB010000016        49494 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJOTB010000016 DAJOTB010000000
VERSION     DAJOTB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.016"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..539
                     /gene="nfeR"
                     /locus_tag="OHN28_000941"
                     /gene_synonym="yqjI"
     CDS             <1..539
                     /gene="nfeR"
                     /locus_tag="OHN28_000941"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCQ2296379.1"
                     /translation="HCGHGHQHEHGQCCGGRHGRGGGRRQRFFGHGELRLVILDILSR
                     DDSHGYELIKAIENLTQGNYTPSPGVIYPTLDFLQEQSLITIREEEGGKKQIALTEQG
                     AQWLEENREQVEMIEERIKARCVGAALRQNPQMKRALDNFKAVLDLCVNQSDITDAQI
                     KKIIAVIDRAAFDITQLD"
     gene            complement(646..2166)
                     /gene="aer"
                     /locus_tag="OHN28_000942"
     CDS             complement(646..2166)
                     /gene="aer"
                     /locus_tag="OHN28_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCQ2296380.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            2584..3963
                     /gene="ygjG"
                     /locus_tag="OHN28_000943"
     CDS             2584..3963
                     /gene="ygjG"
                     /locus_tag="OHN28_000943"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCQ2296381.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKM
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4005..4337)
                     /locus_tag="OHN28_000944"
     CDS             complement(4005..4337)
                     /locus_tag="OHN28_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCQ2296382.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4556..5539
                     /gene="ebgR"
                     /locus_tag="OHN28_000945"
     CDS             4556..5539
                     /gene="ebgR"
                     /locus_tag="OHN28_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCQ2296383.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5723..8815
                     /gene="ebgA"
                     /locus_tag="OHN28_000946"
     CDS             5723..8815
                     /gene="ebgA"
                     /locus_tag="OHN28_000946"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCQ2296384.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
     gene            8812..9261
                     /locus_tag="OHN28_000947"
     CDS             8812..9261
                     /locus_tag="OHN28_000947"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCQ2296385.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9324..10757
                     /locus_tag="OHN28_000948"
     CDS             9324..10757
                     /locus_tag="OHN28_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2296386.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            10891..11961
                     /gene="ygjJ"
                     /locus_tag="OHN28_000949"
     CDS             10891..11961
                     /gene="ygjJ"
                     /locus_tag="OHN28_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCQ2296387.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            11978..14329
                     /gene="ygjK"
                     /locus_tag="OHN28_000950"
     CDS             11978..14329
                     /gene="ygjK"
                     /locus_tag="OHN28_000950"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCQ2296388.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPEGWSPLFNGEATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14555..16573
                     /gene="fadH"
                     /locus_tag="OHN28_000951"
     CDS             14555..16573
                     /gene="fadH"
                     /locus_tag="OHN28_000951"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCQ2296389.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16618..17034)
                     /gene="higA"
                     /locus_tag="OHN28_000952"
     CDS             complement(16618..17034)
                     /gene="higA"
                     /locus_tag="OHN28_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCQ2296390.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17031..17345)
                     /gene="higB"
                     /locus_tag="OHN28_000953"
     CDS             complement(17031..17345)
                     /gene="higB"
                     /locus_tag="OHN28_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCQ2296391.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(17630..18766)
                     /gene="rlmG"
                     /locus_tag="OHN28_000954"
     CDS             complement(17630..18766)
                     /gene="rlmG"
                     /locus_tag="OHN28_000954"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCQ2296392.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18851..19354
                     /locus_tag="OHN28_000955"
     CDS             18851..19354
                     /locus_tag="OHN28_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCQ2296393.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            19431..20123
                     /locus_tag="OHN28_000956"
     CDS             19431..20123
                     /locus_tag="OHN28_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCQ2296394.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            20202..21188
                     /locus_tag="OHN28_000957"
     CDS             20202..21188
                     /locus_tag="OHN28_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2296395.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            21471..22436
                     /gene="alx"
                     /locus_tag="OHN28_000958"
     CDS             21471..22436
                     /gene="alx"
                     /locus_tag="OHN28_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCQ2296396.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            22760..24004
                     /gene="sstT"
                     /locus_tag="OHN28_000959"
     CDS             22760..24004
                     /gene="sstT"
                     /locus_tag="OHN28_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCQ2296397.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(24008..24559)
                     /locus_tag="OHN28_000960"
     CDS             complement(24008..24559)
                     /locus_tag="OHN28_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCQ2296398.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(24642..26129)
                     /gene="uxaA"
                     /locus_tag="OHN28_000961"
     CDS             complement(24642..26129)
                     /gene="uxaA"
                     /locus_tag="OHN28_000961"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCQ2296399.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(26144..27556)
                     /gene="uxaC"
                     /locus_tag="OHN28_000962"
     CDS             complement(26144..27556)
                     /gene="uxaC"
                     /locus_tag="OHN28_000962"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCQ2296400.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            28039..29337
                     /gene="exuT"
                     /locus_tag="OHN28_000963"
     CDS             28039..29337
                     /gene="exuT"
                     /locus_tag="OHN28_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCQ2296401.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            29467..30243
                     /gene="exuR"
                     /locus_tag="OHN28_000964"
     CDS             29467..30243
                     /gene="exuR"
                     /locus_tag="OHN28_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCQ2296402.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            30588..31250
                     /locus_tag="OHN28_000965"
     CDS             30588..31250
                     /locus_tag="OHN28_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2296403.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            31254..31637
                     /gene="mzrA"
                     /locus_tag="OHN28_000966"
     CDS             31254..31637
                     /gene="mzrA"
                     /locus_tag="OHN28_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCQ2296404.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            31784..32152
                     /locus_tag="OHN28_000967"
     CDS             31784..32152
                     /locus_tag="OHN28_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCQ2296405.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            32190..32495
                     /locus_tag="OHN28_000968"
     CDS             32190..32495
                     /locus_tag="OHN28_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2296406.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            32498..32902
                     /locus_tag="OHN28_000969"
     CDS             32498..32902
                     /locus_tag="OHN28_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCQ2296407.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            32892..33191
                     /locus_tag="OHN28_000970"
     CDS             32892..33191
                     /locus_tag="OHN28_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCQ2296408.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            33378..33770
                     /locus_tag="OHN28_000971"
     CDS             33378..33770
                     /locus_tag="OHN28_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2296409.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            33840..34826
                     /gene="yqjG"
                     /locus_tag="OHN28_000972"
     CDS             33840..34826
                     /gene="yqjG"
                     /locus_tag="OHN28_000972"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCQ2296410.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            35109..35477
                     /locus_tag="OHN28_000973"
     CDS             35109..35477
                     /locus_tag="OHN28_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2296411.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(35511..36407)
                     /gene="yhaJ"
                     /locus_tag="OHN28_000974"
     CDS             complement(35511..36407)
                     /gene="yhaJ"
                     /locus_tag="OHN28_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCQ2296412.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            36512..37213
                     /locus_tag="OHN28_000975"
     CDS             36512..37213
                     /locus_tag="OHN28_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCQ2296413.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            37236..37400
                     /locus_tag="OHN28_000976"
     CDS             37236..37400
                     /locus_tag="OHN28_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296414.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(37461..38771)
                     /locus_tag="OHN28_000977"
     CDS             complement(37461..38771)
                     /locus_tag="OHN28_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCQ2296415.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(38799..40130)
                     /locus_tag="OHN28_000978"
     CDS             complement(38799..40130)
                     /locus_tag="OHN28_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2296416.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLM
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(40408..41772)
                     /gene="tdcG"
                     /locus_tag="OHN28_000979"
     CDS             complement(40408..41772)
                     /gene="tdcG"
                     /locus_tag="OHN28_000979"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2296417.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(41844..42233)
                     /locus_tag="OHN28_000980"
     CDS             complement(41844..42233)
                     /locus_tag="OHN28_000980"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCQ2296418.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(42247..44541)
                     /gene="tdcE"
                     /locus_tag="OHN28_000981"
     CDS             complement(42247..44541)
                     /gene="tdcE"
                     /locus_tag="OHN28_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCQ2296419.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(44575..45783)
                     /gene="tdcD"
                     /locus_tag="OHN28_000982"
     CDS             complement(44575..45783)
                     /gene="tdcD"
                     /locus_tag="OHN28_000982"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCQ2296420.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(45809..47140)
                     /gene="tdcC"
                     /locus_tag="OHN28_000983"
     CDS             complement(45809..47140)
                     /gene="tdcC"
                     /locus_tag="OHN28_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCQ2296421.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(47162..48151)
                     /gene="tdcB"
                     /locus_tag="OHN28_000984"
     CDS             complement(47162..48151)
                     /gene="tdcB"
                     /locus_tag="OHN28_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCQ2296422.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(48250..49188)
                     /gene="tdcA"
                     /locus_tag="OHN28_000985"
     CDS             complement(48250..49188)
                     /gene="tdcA"
                     /locus_tag="OHN28_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCQ2296423.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT        12048 a        12940 c        12487 g        12019 t
ORIGIN      
        1 agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc
       61 gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg
      121 atattctctc gcgcgatgac agccacggtt acgagttgat taaagcgatt gaaaatctga
      181 cccaggggaa ttacacccca agcccgggcg tcatctaccc gacgctggat tttctgcagg
      241 agcagtcgct gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg
      301 aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt agagatgatt gaagaacgca
      361 tcaaagcgcg ttgcgttggc gctgcgctgc gccagaaccc gcaaatgaag cgggcgctgg
      421 ataattttaa agcggtgctg gatttatgcg tcaaccagag cgatatcact gatgcacaaa
      481 taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacccaa ctggattaat
      541 caccgattgc cggatgcggc gtaccctaca caccccgtag gcctgataag atgcgccagc
      601 atcgcatcag gcattgtgct ccaaccgccg gatccggcat accgattaat gcagcaccgt
      661 caccgcgtct tccagtcggc tggcgcggtg tttcaccatc gccgacgcct gcgcactctc
      721 ttccactaac tcggcgtttt tctgggtgat caggttaagc tcatccactg cacgggtcag
      781 gctggaaagc ccatcggcct gttccagcgt tgaatggcta atctgagcta tcaactgggt
      841 gacgtttttt acctgcgcca caatatcttc catcgtccgt ccggcggcgt gtacctgctg
      901 cgaaccagat tgcaccttat cagcactggc atcaatcagc ttgcgaatat cgttggcagc
      961 attggcgctg cggctggcta aatgacgcac ttcccccgcc accactgcaa aacctttgcc
     1021 ctgttcgccg gcacgcgccg cttccaccgc cgcattcagg gccagaatat tggtctgaaa
     1081 cgcaatatcg ttaatcagcg aagtaatggt gccaatgcgc tgggtactgt cggcaatatc
     1141 gtccattgtc ttgatcaccg tagtcatcgc ctccccgccc tgcactgccg cattactggc
     1201 ggtgatagaa agtttatcgg cagccgacgc cgtggcagag ttctgtttca ccgacgccgc
     1261 catttggttc atggtcgcca ccgtttgctg aacgttatca actgtctgct gggtatgttc
     1321 gttcagttca tcggtgcctt tcgccagcgt ctcactgccg tttctgacac tggacacctg
     1381 gcttgagaca tcattaatta gccaacggca catcaggcca agctgcccta ccgcgcgtaa
     1441 tgtcagcccc agctcatcgc tgcgcttcag atgctcaaca ctattacgtt ctccggtcgc
     1501 cactttcagt gcctggctgg cgacattttc tatcggacgt acaatttgcc attcaaaaca
     1561 ggcatttccc aacaacatca ctaatgcaca gaagatatac gtcaccaccg gggcagcaac
     1621 aaaccagagc atggccgcca gcacgataaa catcagggtc atcactccac gcgcccgcca
     1681 gcgaagcggt aatgaaggca gtttacccag ccagccttta cgcaccacca ggcctttatg
     1741 aatacgctta ccggtacgtc cggcattcag cgctttgtac agcggctcca ccgccgcgat
     1801 ctcttcatcc gtcgcccggg tacgaatcga catatagcca ctgatttttc cctcgcgcac
     1861 catcggtacc gcattggccc gcacccaata atggtcgcca tttttgcggc gatttttcac
     1921 gatgccgctc cagggttccc cttttttcag ggtgaaccac atatccgcaa acgccgcttt
     1981 tggcatatcc gggtgacgca ccatgttgtg cggttgcccc tgtaactcct gcaaggtata
     2041 gccgctcacc tgcacaaaag tgtcattagc atgagtgata tagctttgca gatcggtagt
     2101 ggacatcaga gtggtgtcgt ccgccagcgg ggtattttgc tgggtgacat acggatgaga
     2161 agacatgttc gcgtcctgtg caggttatat ggttgttaac ttcttgtcag agtttatgtc
     2221 ggccccgctg cggttatctt taaccaatta atttgattta gatcgcaatt tgcgattaaa
     2281 gcacaaatct aattccttga tttaaaatac tttcactctg ttactatacg aaaacgttaa
     2341 ttatcttgcc caaaaatcag gcaattattg ccctgaaagc gtgcatttgc gcagcaatca
     2401 tcaaatccat acccgacaaa aaccgtgcaa aataacagca aatgttagca aatagcatta
     2461 aatattgcgc aaatgataac cacatttgtg tttatcccga ttttcgcgat cgcagccgga
     2521 gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga tacgcgagcc tccggagcat
     2581 attttgaaca ggttaccttc gagcgcatca gctttagcgt gcagcgccca cgccctgaat
     2641 ctcattgaaa agcgaacgtt ggatcatgag gagatgaaag cacttaaccg ggaggtgatt
     2701 gaatacttca aagagcatgt caatccgggg tttttagagt atcgcaaatc tgttaccgcc
     2761 ggcggggatt acggagccgt agagtggcaa gcgggaggtt taaatacgct tgtcgacacc
     2821 cagggacagg agtttatcga ctgcctggga ggttttggaa ttttcaacgt ggggcaccgt
     2881 aatccagttg tggtttccgc cgtacagaat caacttgcga aacaaccgct tcacagccag
     2941 gagctgcttg atccgttacg ggcgatgctg gcgaaaaccc ttgcggcgct aacgcccggt
     3001 aaactgaaat acagcttctt ctgtaatagc ggcaccgaat ccgttgaagc ggcgctgaag
     3061 atggcgaagg cttatcagtc accgcgcggc aagtttactt ttattgccac cagcggcgcg
     3121 ttccacggta aatcacttgg cgcgctgtcg gccacggcga aatcgacctt ccgcaaaccg
     3181 tttatgccac tgctgccagg gtttcgtcat gtaccgtttg gcaatatcga agccatgcgc
     3241 acggctctta acgagtgcaa aaaaaccggt gatgatgttg ctgcggtgat cctcgaaccg
     3301 attcagggcg aaggtggcgt aattctgccg ccgccaggct atctcaccgc cgtgcgtaag
     3361 ctgtgcgatg agttcggcgc actgatgatc ctcgatgaag tacaaacggg catggggcgc
     3421 acgggcaaga tgttcgcctg cgagcatgag aacgtacagc cggatatcct ttgtctggca
     3481 aaagcgctcg gcggcggcgt gatgccgatt ggcgcaacca tcgccactga agaggtgttc
     3541 tcagtcctgt tcgacaatcc gttcctgcac actaccacct ttggcggcaa cccgctggcc
     3601 tgtgcggcgg cgctggcgac catcaatgtg ttgctggagc agaacttacc ggctcaggct
     3661 gaacaaaaag gcgatatgtt gctggacggt ttccgtcaac tggcgcggga atatcccgat
     3721 ctggtacagg aagcgcgtgg taaagggatg ttgatggcga ttgagtttgt tgataacgaa
     3781 atcggctata actttgccag cgagatgttc cgccagcgcg tactggtggc cggaacactc
     3841 aacaacgcta aaacgatccg cattgaaccg ccactgacac tgaccattga acagtgtgaa
     3901 ctggtgatca aagcggcgcg taaggcgctg gcggccatgc gagtcagtgt cgaagaagcg
     3961 taatatcaaa tcggatggcg atacgacgtc gccatccgat ctttttatac gatacgtacg
     4021 cccgcaggca tcatccgctc cggcgttaac agcacgcttt cactgccgtc atcggtttcc
     4081 gcacacaaca gcatgcattc cgacgtttca ccgcgcattt tcgctttttg cagattgcat
     4141 aagaccacca ctgtttttcc catcagctct tcttcgctgt agtacggcac cagactggtc
     4201 acggtttgca gcgttttttc ccccacatca acctgtacga tgtacagctt gtcggcgttt
     4261 tcatggcgtt tcacttccac aatctttccg acgcgcattt ccagacgtgc aaaatcagcg
     4321 taagccacgg tttccattgt ttcctccctt caagtaaaat tttactaaac tatagcaaaa
     4381 gtttttctca atcctgtagg ctaaaaatgg agaatgcagg cgtgatcaca ttcgtgagcc
     4441 gctgtgttac cgtcacagcg tcaaagaaac gcgctttatt tactgaaaac aggtgacccg
     4501 ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg taaacatggc
     4561 aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat ccagggtctt
     4621 gaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc tcgagatcgc
     4681 cgaaaagctg gagtacaaga ccagtagcgc ccgtaaactc cagacaggcg cagtcaacca
     4741 acaccatatt ctggctatct acagctacca acaggagttg gagatcaacg atccttacta
     4801 tctggcgatc cgccacggca ttgaaactca gtgcgaaaag ctggcgatcg aactgaccaa
     4861 ctgttatgaa cacaacggct taccagacat taaaaacgtc accggtattt taattgtcgg
     4921 caaacccacg tccgccctgc gcgccgccgc ctgtgcgttg accgacaata tctgttttat
     4981 cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg cacgtatcag
     5041 taaagaaatc atcgatttct atatcaatca gggcgttaat cgtattggtt ttattggcgg
     5101 tgaagactta cccggcaagg cggatattcg tgaggtcgcc tttgcggaat atggtcgtct
     5161 gaaacacgtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt cgtcgggcta
     5221 tgaactggca aaacagatgc tggcgcggga agactatccg aaggcactgt ttgttgcttc
     5281 cgattccatt gctatcggcg tactgcgggc aattcatgaa cgtggcctga acatcccaca
     5341 ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct ttccgccgct
     5401 ctccaccgtg cgcatccatt ccgagatgat gggaagtcag ggcgttaacc tggtgtatga
     5461 aaaagcccgc gatggtcgcg cgctgccgct gttggtcttc gttcccagca aattaaaact
     5521 gcgcggcacg acccgttaaa tccccttaca cactgtccgg caatcgtttt tgccggacag
     5581 tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttacctgct attttttagt
     5641 aaaaatttta ctaaactccc cagtaattac acaaactacc atcaccataa atggttccga
     5701 tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca cccacgaaaa
     5761 ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcaag cgcgtacctt
     5821 tgcccgcgaa accagcagcc tgtttctgtc cttaagcggt cagtggaatt tccacttttt
     5881 tgaccatccg ctgcaagtac cagaagcctt cacctctgag ttaatggctg actgggggca
     5941 tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaactgcaat ataccgacga
     6001 aggttttccg ttccccatcg atgtgccgtt tgtccccagc gataacccaa ccggtgccta
     6061 tcaacgtatt ttcaccctca gcgacggctg gcagggtaaa cagacgctga ttaaatttga
     6121 cggcgtcgaa acctattttg aagtctacgt taacggtcag tatgtggggt tcagcaaagg
     6181 cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg acaacctgtt
     6241 gtgtgtgcgc gtgatgcaat gggcggactc tacctacgtg gaagaccagg atatgtggtg
     6301 gtcggcgggg atcttccgcg atgtttatct gatcggaaaa caactcacgc acattaacga
     6361 tttcaccgtg cgcaccgact ttgacgaagc ctattgcgat gccacacttt cctgcgaagt
     6421 ggtgctggaa aatctcgccg cctcccctgt cgtcacgacg ctggaatata ccctgtttga
     6481 tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa aactgaccag
     6541 cgcccgcttt gattttactg tcgaacagcc gcagcaatgg tcagcagaat ccccttatct
     6601 ttaccatctg gtcatgacgc tgaaagacgc cgacggcaac gttctggaag tggtgccaca
     6661 acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca ataaccgtta
     6721 tgtgatgctg cacggcgtca accgtcacga caacgatcat cgcaaaggcc gcgccgttgg
     6781 aatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaacatca actccgtgcg
     6841 taccgctcac tacccgaacg atccgcgttt ttacgaactg tgtgatatct acggcctgtt
     6901 tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg atatcagccg
     6961 tattaccgac gatccgcagt gggaaaacgt ctacgtcgag cgcattgttc gccatattca
     7021 cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat ccggctatgg
     7081 ctgtaacatc cgcgcgatgt accatgcggc taaggcgctg gatgacacgc gactggtgca
     7141 ctacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt acacccgcgt
     7201 gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca tctgtgaata
     7261 tgctcatgcg atggggaacg gaccgggcgg gctgacggag taccagaacg tcttctataa
     7321 gcacgattgc attcagggac attatgtctg ggaatggtgc gaccacggga tccaggcgca
     7381 ggatgacaac ggcaatgtct ggtataaatt cggcggcgac tacggcgact atcccaacaa
     7441 ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgcctgggc cgggcctgaa
     7501 agagtacaaa caggttatcg cgccggtaaa aatccacgcg ttggatctga ctcgcggcga
     7561 gttgaaagtc gaaaataaac tgtggtttac cacgctggat gactacaccc tgcacgcaga
     7621 ggtgcgcgcc gaaggtgaaa cgctcgcgac gcagcagatt aaactgtgcg acgttgcacc
     7681 gaacagcgaa gcccccttgc agatcacgct gccgcaactg gacgctcgtg aagcgtttct
     7741 caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccggac accctatcgc
     7801 tacttatcag ttcccgctga aggaaaatac cgcgcagcct gtacctttcg cgccaaataa
     7861 tgcgcgtccg ctgacgctgg aagacgatcg tttgagctgc accgttcgcg gctacaactt
     7921 cgcaatcacc ttctcaaaaa tgagtggcaa accgacatcc tggcaggtga atggcgagtc
     7981 gctgctgacc cgcgagccaa agatcaactt cttcaagcca atgatcgaca accacaagca
     8041 ggagtacgaa gggctgtggc agccgaatca tttgcagatc atgcaggaac atctgcgcga
     8101 ctttgccgta gaacagagcg atggtgaagt gttgatcatc agccgcacgg ttattgcccc
     8161 accggtgttt gacttcggga tgcgctgcac ctacatctgg cgcatcgctg ccgatggcca
     8221 ggttaacgtg gcgctttccg gcgagcgtta cggcgactat ccgcacatca ttccgtgcat
     8281 cggtttcacc atggggatta acggcgaata cgatcaggtg gcgtattacg gtcgtggacc
     8341 gggcgaaaac tacgccgaca gccagcaggc taacatcatc gatatctggc gcagcaccgt
     8401 cgatgccatg ttcgagaact atcccttccc acagaacaac ggcaaccgtc agcatgtccg
     8461 ctggacggca ctgactaacc gccacggcaa tggtctgctg gttgttccgc agcgcccaat
     8521 taacttcagt gcctggcact acacccagga aaacatccac gcagcccagc actgtaacga
     8581 gctgcagcgc agtgatgaca tcaccctgaa tctcgaccac cagttgcctg gcctcggctc
     8641 caactcctgg ggcagcgagg tgctggactc ctggcgcgtc tggttccgtg acttcagcta
     8701 cggctttacg ttgctgccgg tttctggcgg agaagctacc gcgcaaagcc aggcgtcgta
     8761 tgagttcggc gcagggttct tttccacgaa tttgcacagc gagaataagc aatgaggatc
     8821 atcgataact tagaacagtt ccgccagatt tacgcctctg gcaagaagtg gcaacgctgc
     8881 gttgaagcga ttgaaaatat cgacaacatt cagcctggcg tcgcccactc catcggtgac
     8941 tcattgactt accgcgtgga gacggactcc gcgaccgatg cgctatttac cgggcatcga
     9001 cgctattttg aagtgcatta ctacctgcaa gggcagcaaa aaattgaata tgcgccgaaa
     9061 gagacattac aggtagtgga atattatcgt gatgaaactg accgcgaata tttaaaaggc
     9121 tgcggagaaa ccgtagaggt ccacgaaggg caaatcgtta tttgcgatat ccatgaagcg
     9181 tatcggttta tctgcaataa cgcggtcaaa aaagtggttc tcaaagtcac catcgaagat
     9241 ggttatttcc ataacaaata aaaactacgg cggcaaaagg agtttgccgc caccgctacc
     9301 ctactcattt tcggagatgt gttatgtctg ataccaaacg taatacaatc ggcaaattcg
     9361 gcttgctctc gctgactttt gccgccgttt acagctttaa caacgttatc aataataata
     9421 ttgagcttgg actggcctcg gcaccgatgt ttttcctcgc aacaattttt tattttattc
     9481 ccttctgtct gatcatcgca gaatttgttt cgttaaataa aaactcggaa gccggtgtct
     9541 acgcgtgggt aaaaagttcg ctgggcggac gttgggcatt tattactgcc tatacctact
     9601 ggttcgtaaa cctgttcttt ttcacctcat tattgccgcg cgttattgct tacgcctcgt
     9661 atgcattcct cggctacgaa tatattatga cgccggttgc caccaccatt atcagtatgg
     9721 tgctgttcgc cttttccacc tgggtttcta ccaacggggc gaaaatgctg gggccaatta
     9781 cctccgtcac ttcaacgctg atgctgctgt taacgctctc ctacatttta ctggcaggta
     9841 cggcactggt tggcggcgta cagcctgctg accccatcac tgttgacgcg atgatcccaa
     9901 actttaactg ggcgttcctc ggcgttacca cctggatctt tatggctgca ggtggcgcgg
     9961 agtccgtcgc ggtgtacgtt aacgacgtca aaggcggttc gaaatcgttc gttaaagtga
    10021 tcatcctcgc cgggattttt attggcgtac tgtactccgt ctcctcggta ctgattaacg
    10081 tcttcgtcag cagcaaagag ttgaagttta ccggcggatc ggtacaggta ttccacggca
    10141 tggcggcgta ttttggtcta ccggaagcac tgatgaatcg ctttgtcggt ctggtgtcct
    10201 ttaccgcgat gttcggttcc ctgctgatgt ggacggcaac gccggtgaaa attttcttct
    10261 ccgaaattcc ggaaggcatc tttggtaaga aaaccgtcga actgaacgaa aacggcgttc
    10321 cggcgcgcgc agcgtggatc cagttcctga tcgtcatccc gctgatgatt atcccgatgc
    10381 tcggttccaa taccgtgcag gatctgatga atactattat taatatgact gctgcagcgt
    10441 ccatgcttcc gccgttattc atcatgctgg cttacctgaa tttacgcgcc aaattagatc
    10501 acctgccacg cgatttccgt atgggctccc gccgcaccgg tattatcgtt gtttcaatgc
    10561 tgattgcgat atttgcagta gggtttgtcg cttcgacatt cccgactggc gcgaatattc
    10621 tgaccatcat tttttataac gtcggcggta ttgttatctt cctcggcttt gcgtggtgga
    10681 aatacagtaa atatataaag ggattaacgg ctgaagagcg ccatattgaa gcgacgccag
    10741 ccagcaatgt tgattaagca gaataataaa aaatgagcag gcattttccc tcttcctgga
    10801 tggggagagg gaaaatagtt tctgccttta tatttttata attacaacga taaaaggctg
    10861 tactttttct ttagctcatg gattaacaca atgaaattaa tcactgcacc atgcagagca
    10921 ttacttgccc tgcctttttg ctacgctttt tctgcacttg gcgaagaagc acgtccggca
    10981 gaacatgacg acacaaaaac ccccgaaatg atctcgacat cttctccttc atttcgtttt
    11041 tacggcgaat taggggttgg tgggtatatg gatttagagg gtgagaataa acataaatac
    11101 agcgacggca cctatattga aggtggcctg gagatgaagt acgactcctg gttcggcctg
    11161 atttacggcg aaggctggac cgtgcaggcc gaccacgacg gtaatgcctg ggtgcctgac
    11221 catagctggg gtggtttcga aggtgggatt aaccgtttct atggcggtta tcgtaccaat
    11281 gatggcaccg aaatcatgct cagtctgcgt caggattcct cgctggatga cctgcaatgg
    11341 tggggcgatt tcacgcccga tctgggctac gtcattccca atacccgcga cattatgact
    11401 gcgctgaagg tacagaactt aagcggcaac tttcgttata gcgttaccgc cacacctgcc
    11461 ggacatcatg acgaaagcaa agcctggcta cattttggca agtacgatcg ctacgacgac
    11521 aaatacacct atccggcaat gatgaacggt tacatccagt atgaccttgc agaaggtatc
    11581 acctggatga acggtctgga aatcaccgac ggcacaggcc aactctatct cacgggcctg
    11641 cttaccccta actttgccgc tcgcgcctgg caccataccg gacgcgccga cgggctggac
    11701 gtaccgggaa gtgaaagtgg aatgatggtg agcgccatgt atgaagcgtt aaagggcgtt
    11761 tatctctcca ccgcttacac ctacgccaaa catcgccctg accacgctga cgatgaaacc
    11821 acctctttca tgcagtttgg tatctggtac gaatacggcg gcggacgttt cgcaacggct
    11881 tttgatagcc gcttctacat gaaaaatgcc tctcacgatc ccagcgacca actcttcctg
    11941 atgcaatatt tttactggta ataaggacag taatctcatg aaaatcaaaa ctattttaac
    12001 accagtagcc tgtgctctgc tgatgagctt ttccgcccat gccgctaacg ctgacaatta
    12061 taaaaacgtg attaaccgta ctggcgcgcc gcagtacatg aaggattacg attacgacga
    12121 tcaccagcgt tttaatccgt ttttcgatct cggagcctgg catggtcatc tgttgccaga
    12181 cggcccgaac actatgggcg gctttccagg cgttgcgttg ttgacggaag agtacatcaa
    12241 ctttatggcc agcaatttcg accgcctgac cgtctggcag gacggcaaga aagtcgattt
    12301 cacgctggag gcatacagta ttcccggcgc gctggtgcaa aagttgatat cgaaagatgt
    12361 acaggtcgaa atgattctgc gcttcgccac gccgcgcaca tcactactgg aaaccaaaat
    12421 caccagcgat aaaccgctgg atctggtgtg ggatggcgaa ctgctggaaa aactggaagc
    12481 gaaagaaggg aaaccgcttt ccgataaaac cattgctggc gagtaccctg actatcagcg
    12541 caaaatcagc gccacccgcg atggcctgaa agttaccttt ggcaaagtgc gcgccacctg
    12601 ggatctgctg acctccggtg aatcagaata tcaggtgcat aaatcattac cgatgcagac
    12661 tgaaatcaac ggcaatcgct ttaccagtaa ggcgcatatc aacggatcga cgacgctcta
    12721 taccacctat tcccatctgc tgaccgctca ggaagttagc aaagagcaaa tgcagatccg
    12781 cgatattctg gcgcgtccgg cgttttatct caccgcctcg cagcaacgtt gggaagaata
    12841 cctgaagaaa ggattaacca atccggatgc gacgccggaa cagacgcgcg tcgcggtgaa
    12901 agccatcgaa acgctcaacg gtaactggcg atcgccgggc ggtgcggtga aatataacac
    12961 cgtcacgccg tcggtgaccg ggcgctggtt ctccggcaac cagacctggc cgtgggatac
    13021 ctggaagcag gcgtttgcga tggcgcattt caatccggac atcgccaaag agaatatccg
    13081 cgcggtcttc tcctggcaga tccagccagg cgaccgcgtg cgtccgcagg atgtgggctt
    13141 tgtccccgac ctgatagcgt ggaaccttag ccccgagcgt ggcggcgatg gcggcaactg
    13201 gaacgaacgt aataccaaac ccagccttgc cgcctggtca gtaatggaag tgtataacgt
    13261 cacccaggat aaggcctggt tggcagagat gtacccgaag ctggtggcct atcacgactg
    13321 gtggttacgt aaccgcgatc acaacggcaa cggcgtgccg gaatatggcg cgacccgcga
    13381 caaagcccac aacaccgaga gcggcgagat gctgtttacg gtgaaaaaag gcgacaaaga
    13441 ggagacgcag tctgggctga acaactacgc ccgtgtggtg gagaaaggcc agtacgacag
    13501 tctggaaatt ccggcacagg ttgctgcgtc gtgggaatca ggtcgtgatg acgccgccgt
    13561 tttcgggttt atcgacaaag aacagctgga taaatatgtc gctaacggcg gcaaacgtag
    13621 cgactggacg gtgaaattcg ccgaaaaccg cagtcaggac ggaacgttgc tgggctactc
    13681 gctattgcag gagtcggtgg atcaggccag ctatatgtac agcgataacc attatctggc
    13741 ggagatggcg acgattctcg gtaagccgga agaggccaaa cgctatcgtc agttagcaca
    13801 acagctcgcg gactacatca acacttgtat gttcgacccg actacacagt tctactatga
    13861 cgtgcgtatt gaagataaac cgctggcgaa cggctgcgcg ggcaaaccga ttattgagcg
    13921 cggtaaaggg ccggaaggct ggtcgccgct gtttaacggt gaggcaacgc aggccaatgc
    13981 cgacgcggtg gtgaaggtga tgctggatcc gaaagaattc aacacctttg tcccgctggg
    14041 aacggcggcg ttaaccaatc cggcttttgg cgcggatatc tactggcgcg ggcgcgtatg
    14101 ggtggatcag ttctggtttg gtctgaaagg gatggagcgt tacggttatc gtgatgatgc
    14161 cctgaagctg gcggatacgt tcttccggca cgccaaagga ttaaccgccg atggcccaat
    14221 tcaggagaat tacaacccgc tcactggctc gcaacaaggc gcaccaaatt tctcctggag
    14281 tgccgcgcat ttgtatatgt tgtataacga ttttttccgt aagcagtaat ttgcaacggc
    14341 tggcggatgc ggcgtgaacg ccttatccgc cctacatgtg caatccccca ggccggataa
    14401 aacgcggcaa gcgtcgcatc cggcgttacc accgggcgta ttctttttga atcccatcac
    14461 aaaccccgca ctcccctttt cccttttctc cggcgacggc taaattagaa ctcatccgac
    14521 cacataacaa ttattttaca tactggacaa ttttatgagc tacccgtcgc tgttcgcccc
    14581 gctggattta ggttttacca cgttaaaaaa ccgcgtgttg atgggctcaa tgcacaccgg
    14641 gctggaggag tacccggacg gcgctgaacg gctggcagcg ttttatgccg aacgcgcccg
    14701 tcacggcgtg gcgttgattg tcagcggcgg tatcgcacca gatttaacag gcgttggcat
    14761 ggaaggcggt gcaatgctca atgacgccaa ccagatccca caccatcgca ccattaccga
    14821 agccgtacat caggaaggcg gcaaaatcgc cctgcaaatt ttgcataccg ggcgctacag
    14881 ctaccaaccg catctggtcg ctccctccgc attgcaggcc cccatcaacc gtttcgttcc
    14941 tcacgaactc acccatgaag agatcctgca actgatcgac gatttcgccc actgcgcgca
    15001 attggcacgg gaggcaggat acgacggtgt agaggtgatg gggtccgaag ggtatttgat
    15061 caacgaattt ctgacgctgc gcaccaatca gcgtagtgac cagtggggcg gcgattaccg
    15121 caaccggatg cggtttgccg tagaagtagt gcgtgcggtg cgcgaacgtg tcggcaacga
    15181 cttcattatt atctaccgtt tgtcgatgct cgacctggtc gaaggcggcg gtacttttgc
    15241 cgaaatggta gagctggcgc aggccattga agcggcgggc gcgaccatta tcaacaccgg
    15301 cattggctgg catgaagcgc ggattccgac catcgccacg cccgtgccgc gcggcgcctt
    15361 tagctgggtc acgcgcaaac tgaaagggca cgtttcgctg ccactggtga ccaccaaccg
    15421 gattaacgat ccgcaggttg ccgacgacat tctctcgcgc ggtgatgcgg acatggtatc
    15481 gatggcgcga ccgtttctcg ctgatgcgga gctgctatca aaagcgcaat cgggacgagc
    15541 cgatgagatc aacacttgta ttggctgcaa ccaggcctgt ctcgatcaga tcttcgttgg
    15601 caaagtcacc tcgtgcctgg tgaatcctcg cgcctgccac gaaaccaaaa tgccaatcct
    15661 tcctgccgtg cagaaaaaaa atctggcggt ggtcggtgcg ggacctgctg ggctggcgtt
    15721 tgccattaac gcggcggcgc gtgggcatca ggtaacattg tttgatgcgc acagcgagat
    15781 cggcgggcag tttaatatcg ccaaacagat ccccggcaaa gaggagtttt acgaaacgct
    15841 gcgctattac cgccggatga tcgaagtgac gggtgtaacg ctaaaactca atcacaccgt
    15901 gacggcggat cagttacagg cgttcgatga aacgatcctc gccagtggga tcgtgccgcg
    15961 cgttccgcct atcgacggga tcgatcatcc gaaggtactg agttatctcg atgtactgcg
    16021 cgacaaagcg ccggtgggca acaaagttgc catcatcggt tgtggcggaa ttggttttga
    16081 tacggcgatg tatttaagtc agccgggcga atccaccagc cagaatatcg ccgggttctg
    16141 taatgagtgg ggaatcgaca gtagcctgca acaggccgga ggtttgagtc cgcaaggcat
    16201 gcaaatcccc cgtagcccac ggcagattgt gatgctccag cgcaaagcca gcaaaccagg
    16261 acaagggtta ggcaaaacca ccggctggat ccatcgcacc accctgctct cacgcggcgt
    16321 gaaaatgatc ccaggcgtaa gttaccagaa gatcgacgat gacgggctgc atgtagtgat
    16381 caacggcgaa acgcaggtac tagcagtgga caatgtggtg atctgcgcgg ggcaagagcc
    16441 aaaccgcgca ttggcgcaac cgctgattga tagtgggaaa actgtgcatt taattggcgg
    16501 ctgcgatgta gctatggagc tggacgcacg gcgggcaatt gcccagggaa cacggctggc
    16561 gctggagatt taaatcgttg tttgaagcgc cggatgcgac gcatccggca cgtcgcgtta
    16621 atcaataaac aaggcgggag aaataccgaa tcgcgttgcc agttttttag cgtgttccag
    16681 cgttaatttc cttttcccgc tcaaaacgcg tgacaccatc gatttactgc ctatttccgg
    16741 aagatcggaa agggttaaac catattgatc cataagggta cgaatcacgg ctataccgcc
    16801 aggcatggct tgagccatgg cattaaattc cacaaattcg ggcgctgatt cttcccacgc
    16861 ggttattttg gcacacacca gatccagtaa ggggttttca ggatcgttga gcagcagatg
    16921 atctaccagt tccagcgcct gggtgtattg ttcctcgttc tgaatacccg ccagaaaagg
    16981 tgccacagca gttagctttt ctcctgcttg caagatgtcg gcaatcgcaa tcattttttc
    17041 cccttagtac gatgaacggc ggtaaagaaa tcgtattctt tatgcgtcat aacttcacgt
    17101 atgtagcact tttgcgattc aaaaaagacc attgctacaa cacgtaattc attgcccccg
    17161 acattgaaaa cataatgctt atccagatat ttgaagttat ccagagaagg gaatactgct
    17221 tttaatgact caggtttttt gaaatatccc ttagcaatcg tgttccccag agccaccaac
    17281 tccgttttat gttgcgggta tttttccgca gcatctttca atgctttttg agttatcagg
    17341 tgcattcttc atcacgtccg ttgacaaatt ggcaatatga taacatccgt tgacagattg
    17401 gcaacgaatg aatttatctg tgtgacggaa gaataaacgt agaaaaacgc taaacaagag
    17461 aatgtagagg atggtgacag gaattttttg ttttatggaa gcgcgattcc agaactgggc
    17521 agacattgcc ggatgcgacg cttgccgcgt cttatccgac ctgggggtaa tgtgctgaac
    17581 gtatgccgga taaggcgttt acgccgcatc cggcaggtaa cccccaatct taccgacgac
    17641 gccccagctt cactgctttc agcaccacaa atttattatt cgtggcgatg gtggtgcagt
    17701 tgccgaaaat tttcttcagt ttatggaagt aatccaagtg acggttggca acgatataca
    17761 gctcgccgtt gattttcagg cagcggcggg cgtggtggaa catctcccag gcgacgttat
    17821 cggtcagcgc atgttgctga tgaaacggcg ggttgcagag cacagcatta aaacggaaag
    17881 gctccacgcc ggaaagcgcg ttgttgatca taaactcgca gcgatccagt gcctcgggca
    17941 tgttggtttc aacgttcaaa cggctggaag caaccgccat cggtgattca tcgacaaaca
    18001 ccactttcgc ctgcgggttt ttatcgagca atgtcaggcc aataacgcca ttaccgcaac
    18061 cgagatcgac aatctcacct tcgagattct ctggcagatg ttgcataaag aagcgcgcgc
    18121 caatatccag cccggtgcgg gagaagacat tcgcatggtt gtggatagtc cagtcagtac
    18181 cttccagctt ccagctaacg gtctgcggcg catcggccaa cggcggttcg ttgaaggtgc
    18241 agttaatcag gcgcgctttc ttccatgcca gcgtggtggt ggtcgggccg agcacttttt
    18301 cgaacaattc cagcgtggaa gtgtgaatgt cacgggcttt ggcaccagca ataatgcgcg
    18361 tatctggcgt gaccactttg cgcagcgcgc gcagttgttg ttccagcaat gccagtgttt
    18421 tcggcacttt gatcagcacc acacccggct gttgcgggta gtcggcggtg ctgtcgagaa
    18481 acttcacgct cgactcatca atcccgttga ggcgtaaatt ctcgcgcgtg gccagttcac
    18541 tgatgtatga gtcgccaatg ctgtacggct tatgttccgc cagcacacag cttaacgcac
    18601 caaaagcatc attcaggatc aacaccgggc cgcggatttc tgtgtcgtcc aactgttgca
    18661 gcaaatactc atccgccgct tcccacgcct gtagcgggtt aacgtcatcc gtcgccggaa
    18721 aacgttgtag tgtcagtgaa cggaaaccgt tgtctaagtg gctcatcggc cctcctgaat
    18781 gataaaattt cgacgttgcc cctgaaaagg gtgcgtgagt ataccctttt tctctttttc
    18841 gtgggcggtt atgagcaatc ttacttatct tcagggttat cccgagcagc tactttccca
    18901 ggtgcgaacg ctgattaacg aacagcgtct gggcgatgtg ctggcaaaac gttatccggg
    18961 aacacacgac tacgccaccg ataaagccct ctggcaatat actcaggatc ttaaaaatca
    19021 gtttctgcgc aatgccccgc cgatcaataa agtgatgtat gacaataaga tccacgtgct
    19081 gaaaaacgcg ctcgggttac atactgccgt ttcgcgtgtg cagggcggca agctgaaagc
    19141 aaaagcagag atccgcgtcg ctaccgtgtt tcgcaacgcg ccggaaccgt ttttgcgcat
    19201 gatcgtggtg cacgagctgg cgcacctgaa ggagaaagag cataacaaag cgttttacca
    19261 gttgtgctgt catatggaac cgcagtacca ccagcttgag ttcgacaccc ggctgtggct
    19321 aacgcagttg tcgcttgggc aggacaaaat ctgaaaatga tgtcaggcgt tattttttcc
    19381 tcctttagcg cctaaaatcg acctcctccc tttcgttaag gataacgacc atgctgcgcg
    19441 catttgcccg ccttcttctt cgtatttgtt tttctcgccg gactcttaaa attggctgtt
    19501 tattgttgct tatagcagga gcgaccattt ttattgccga tcgcgttatg gtcaacgcca
    19561 gcaagcagct gacctggagt gatgtcaacg ccgttccggc gcggaacgtg ggtttattgc
    19621 tgggggcgag gccgggtaat cgctacttta cccgacgtat tgatagcgcc gcagagttgt
    19681 accacgccgg aaaagtgaaa tggctattgg tgagcggcga taatggacgt aaaaattacg
    19741 atgaagcgtc aggaatgcag caagccttga tcgctaaagg cgtaccggca aaagccatct
    19801 tctgcgacta tgccggattc tcaacgcttg attcggtggt gcgtgccaac aaggtgttta
    19861 gtgaaaacca tatcaccatt atctcgcaag aatttcataa ccaacgcgcc atctggctgg
    19921 caaaacagta tggtatcgat gctatcggtt ttaacgcgcc agacctgaat atgaagcacg
    19981 gtttatatac tcaactgcgg gaaaaacttg ccagagtcag cgcggtgatt gatgcgaaaa
    20041 tcctccatcg ccagccgaaa tatctcgggc cgttggttat gattgggccg tttagtgagc
    20101 atggctgtcc ggcaaaagaa taatgcgtat ctgcgcacgt cgaagataaa aaaggcgtgc
    20161 tacagtgacg acagaatccc tttatggagt atccacgcgt tatgatacgt ttcgctgtga
    20221 ttggtacgaa ctggatcact cgccagttcg tcgaggccgc ccatgagagc ggtaaataca
    20281 agttaaccgc cgtctattcc cgcagccttg aacaggccca gcacttcgcc aatgattttt
    20341 ctgtcgagca tctgtttacg tcgctggaag cgatggcaga aagcgatgcc attgacgcgg
    20401 tgtatattgc cagcccaaat tccctgcact tttcccagac acaacttttc cttagtcaca
    20461 aaattcatgt gatttgcgag aagccactgg cgtcgaatca ggcggaagtg gatgccgcca
    20521 ttgcctgtgc gcgggaaaat caggtggtgc tgtttgaggc atttaaaacg gcctacctgc
    20581 cgaactttca tctgttacgc caggcgctgc cgaaagtcgg caaactgcgt aaagtctttt
    20641 tcaactattg ccagtattcc tcgcggtatc agcgttacct ggacggagag aatcccaaca
    20701 ccttcaatcc ggcattctcc aacggttcga ttatggatat cggcttttac tgcctggcgt
    20761 cggcggtggc attattcggt gaaccgaaaa gcgtgcaggc aaccgccagt ttgctggcaa
    20821 gcggtgtgga tgcccacggc gtggtggtga tggattacgg tgatttcagc gttacgttgc
    20881 agcactcaaa agtgagcgat tctgtcctgg caagcgagat tcagggcgaa gcgggatcgc
    20941 tggtgattga aaaactctct gaatgccaga aagtctgctt tgtaccgcgt ggcagccaga
    21001 tgcaggatct cacccagcca cagcatatta ataccatgct ctacgaagca gagctgttcg
    21061 ctaccctggt ggatgagcat ctggtgaatc atccagggct ggcggtcagt cgtatcaccg
    21121 ccaaactgct gactgagatc cgccgccaga ctggggtgat ttttccggca gataacgtaa
    21181 aactataatt gccaaagtaa aacagtgtaa aaggtatgta acagaccatt gactgactga
    21241 atggtctgtc atactttgtt acctgcaaag gggagtaact tcattgtcgg tcgatcgtca
    21301 ttacggtgtg tgaaaaacca catccggtca ccgggcaacc cgaaaggaat acgcagacgt
    21361 attccttttt tgttgtaagt gagaccttgc cggaaggcga ggtctatgca taaaaagcag
    21421 cggctgacgt cttccgacgt tggccgtttt tttatgtgta aggaacttct atgaatactg
    21481 tcggcacgcc gttgctatgg ggcggattcg ctgttgttgt caccattatg ctggctatcg
    21541 acctgttgtt gcaggggcgt cgtggggcac atgccatgac catgaaacag gctgcggcct
    21601 ggtcgctggt ctgggtgacg ctgtcgttac tgtttaacgc cgctttctgg tggtatctgg
    21661 tgcaaaccga aggtcgcgcc gttgccgatc cacaggcact ggcctttctc acgggttatc
    21721 tgattgagaa atcgctggcg gtcgataacg tctttgtctg gctgatgttg ttcagctatt
    21781 tctctgttcc ggcggcatta caacgccgcg tgctggtgta tggcgtactc ggggcgattg
    21841 ttctgcgtac catcatgatc ttcactggta gctggctgat ttcacagttc gactggatct
    21901 tgtatatctt cggtgccttc ctgctgttta ccggcgtgaa gatggcgctc gcccatgaag
    21961 atgaatcagg cattggcgac aagccgctgg tgcgctggct gcgcggtcat ttgcgcatga
    22021 ccgacaccat cgacaacgag catttctttg tgcgtaagaa tggtttgttg tatgccacgc
    22081 cgctgatgct ggtgctgatt ctggtggagt tgagcgacgt gattttcgcc gtggatagta
    22141 ttccggctat cttcgccgtg accaccgatc cgtttattgt gctgacctca aacctgtttg
    22201 cgatcctcgg cctgcgtgcg atgtatttcc tgctggcagg tgtggcagag cgtttctcga
    22261 tgctcaaata tggtctggcg gtgattctgg tgtttatcgg tatcaagatg ctgattgtcg
    22321 acttctacca tattccgatc gccgtctcgc tgggcgtggt gtttggcatt ctggtgatga
    22381 cgtttattat caacgcctgg gtgaattatc ggcatgataa gcagcgggtt ggatgatttt
    22441 taatctgcct aagccgtgta ccctgtcatc cacatgagca ccgttttctc cctctcccca
    22501 aaaggggcga ggggactgac cgagtacttt tttgacgttg tcatcagtct ggaagccgca
    22561 cgttgggctt tattttcatg tcaaacaaat gtaaccatta agttgcgaaa tatgacttct
    22621 ccttaaaatc cagcatattt cgcttcccga agctgtaact ttccttatac tcgaccttgc
    22681 aaacactttg ttacatcctg aaagatgcgt cgacagaacg caccagggat gtgcgacaac
    22741 acaatgaaag gatcgaaaaa tgactacgca acattcaccg gggctattcc ggcgtctggc
    22801 tcatggcagc ctggtaaaac aaatcctggt cggccttata ctggggattc ttctggcatg
    22861 gatctcaaaa cccgcggcgg aagctgttgg tctgttaggt actttgttcg tcggcgcact
    22921 gaaagccgtt gcccccatcc tggtgttgat gctggtaatg gcatctattg ctaaccacca
    22981 gcacgggcag aaaaccaata tccgccctat tttgttcctc tatctgctgg gcaccttctc
    23041 tgctgctctg gccgcagtaa tcttcagttt tgccttccct tctaccctgc acttgtccag
    23101 tagcgcgggt gatatttcgc cgccgtcagg cattgtagaa gtgatgcgcg ggctggttat
    23161 gagcatggtt tccaacccca ttgatgcgct gctgaaaggt aactacatcg ggatcctggt
    23221 gtgggcaatt ggcctcggtt tcgcactgcg tcacggtaac gaaaccacca aaaacctggt
    23281 caacgatatg tcgaatgccg tcacctttat ggtgaaactg gtgattcgct tcgcaccgat
    23341 cggtattttt ggtctggttt cttctaccct ggcaaccacc ggtttctcca cgctgtgggg
    23401 ctacgcgcaa ctgctggtcg tgttggttgg ctgtatgtta ctggtggcgc tggtggttaa
    23461 ccccttgctg gtgtggtgga aaattcgtcg taacccgttc ccgctggtgc tgctgtgcct
    23521 gcgcgaaagc ggcgtgtatg ccttcttcac ccgcagctct gcggcgaaca ttccggtgaa
    23581 tatggcgctg tgtgaaaagc tgaatctgga tcgcgatacc tattccgttt ctattccgct
    23641 gggagccacc atcaatatgg cgggcgcagc aatcaccatt accgtgttga cgctggctgc
    23701 agttaatacg ctgggtattc cggttgatct gcccacagcg ctgctgttga gcgtagtggc
    23761 ttctctgtgt gcctgtggcg catccggcgt ggcgggggga tctctgttgc tgatcccact
    23821 ggcctgtaat atgttcggta tttcgaacga tatcgccatg caggtggttg ctgtcggctt
    23881 tattatcggc gtattgcagg actcctgtga aaccgcgctg aactcgtcaa ctgacgtgct
    23941 gttcactgcg gcagcttgcc aggcggaaga cgatcgtctg gcaaatagcg ccctgcgtaa
    24001 ctaatactta gcccctttcg tctacggcgg aaggggtttt ctccacttta aacggatcaa
    24061 ttccccttct ctgcatacgc cagaaacgaa tgatattcag gccattcatc agcagaaaac
    24121 tgccttcaat catcgtgccg cctatcgacc ccgcccagaa gttgtgaatc acccagcaac
    24181 acgttgaaaa ccacattacg cagcgcatgg tcagcccttt acagcggaac agcgcccagg
    24241 tactgacaat cgtgccgatt accggcaata gttcaacagg atgatggaac ttcgcgaggc
    24301 caattccgcc agtcagcaca ataaaaatcg ccattaccca taagctgcgc gtgcgtaagg
    24361 taatcaatgt acgaatggca ttaaggatgg cactggcacc ggcaggatag gtgcccagaa
    24421 gaaaaaaatg tacgccaata acggcgctat agaccgaaag ttgctttttg aagcgacgtt
    24481 cgtcacgatt gaaaaatgtt gtgataccaa tcagaaaggc gatgacaccc acgccctggg
    24541 ccagccaata cgcggtcatg ataaatcctt agcaggtatg gaaaagcaaa cggcgcttca
    24601 cattatgaaa cgccgttttt tattaacaac tcatttcgac tttatagcgt tacgccgctt
    24661 ttaaagatcg ccagttcgcg gaagtcgtta cgctcgttac aggtttgctt accgttggca
    24721 aactcaacga tggtgtcgat aaattcttcc agcaactgcg gcatcgcttt accgtggatc
    24781 agctgacccg cgtcaaagtc gatccagtgt tttttcttcg ccgccagttc actgttggtg
    24841 gcgattttca ccgtcggcac aaatccacca tacggcgtgc cacgaccagt actgaacagc
    24901 accatatggc agcccgcacc cgccagggcg ctggtcgcta cggcatcgtt acccggcgca
    24961 cttaacaagt tcagccccgg cgttttcaga cgctcgccgt aacgcagcac gtcaaccacg
    25021 acactggaac ccgctttctg ggtacagcca agcgatttat cttccagcgt ggtgataccg
    25081 cctgctttgt tccccggtga tgggttttcg tagatcggct ggtcatgggc aataaagtac
    25141 tgcttgaagt cattgaccat ggtgaccagt ttttcaaacg ttgcttcgtc gcggcaatgg
    25201 tccatcagca actgctctgc gccaaacatc tccggcactt cggtcagtac ggtagtaccg
    25261 ccgttagcaa tcacgtagtc agagaaacgc cccagcatcg ggttagcagt aataccagaa
    25321 agaccgtcag aaccaccgca ctccagacca aacttcagtt cgctgagttt gcccggctcg
    25381 cgtttatcgt tgcgcatcac gctatacagc tgatgcaaat gcgcgatccc ggcctcgatt
    25441 tcatcatcct gctgttggca gatcatgaaa tgaacgcgtt caggatcgat atcgcccaac
    25501 gtttcacgga atgcggcaac ctggttattt tcgcaaccca gaccaatcac cagcactgcg
    25561 cccgcgttcg ggtggcgcac catgttttgc agcatggtgc gggtattgat gtgatcgtcg
    25621 cccagttgtg agcagccgta ggtgtggctg aagaggaaca cgccgtcggt accttcagcg
    25681 ttgttggtct ctttcaggaa acgattttgg atctgccgcg cgatgccgtt gacacagccc
    25741 acggttggca ggatccacag ctcattacgc accccgacat caccgttagc gcgacgatag
    25801 atctgcactt cacgatctgc cgcttgcgca ggcagatcct gaaaatcagg ttgatagcga
    25861 tactgatcca gatcgctcag attcgtgcgc gtattgtggg cgtgaacgtg ttcccccgcc
    25921 gcaatatccg ccaatgcata accaatcggc aggccatatt taatgacgtt ggcccctttt
    25981 gcgatatccg ttaacgcaaa tttatgtcca cgagcaacat cctggcgcag cgtaacagtc
    26041 tggttatcga cactgacttc tgtgccttca gccaaatccg ctaaagcaac cgcgacgtta
    26101 tccagcgcat ggatcttgat gtattgcata tcaaccccag accttagttc agttcaatgg
    26161 cgaagtagtc acgcgcattg ttaaagcaaa tatttttcac catctcgccc agcaggttga
    26221 tgtccgccgg tgcttcgcct gcttccaccc agcgaccgat catctggcac agaatgcggc
    26281 ggaagtattc gtgacgggtg tatgacagga agctacggct gtcagtcagc atgccgacaa
    26341 agcggctcag cagaccgagc tgcgccagtt gggtcatctg acgttccata ccgtctttct
    26401 ggtcgttaaa ccaccagccg gaaccgaact gcattttgcc cggcatacct tcgccctgga
    26461 agttaccgat catggtgccc agcacttcgt tatcgcgcgg gttcaggcag tagagaatgg
    26521 ttttcggcag caggttttct tcgttctgct tgctcagcag cttagatagc tcttccgcca
    26581 tcggacggtc gttgatggag tcaaagccga catccggccc cagcagtttg aactgacgca
    26641 ggttgttatt acgcagtgcg ccaatgtggt actgctgtac ccatccgcga cgtgcatatt
    26701 cggcaccaag gaacaccagc accgcagttt tgaactgtgc tacttcgtgc tcgctcaggg
    26761 tttcgcctgc cagacggcgc gcgaggatgc tgtccagttc cgcttcgttc gcttcagcaa
    26821 acatcaccac atccagcgcg tggtcagaca ctttacagcc gtgagcggcg aagtgatcca
    26881 gacgtttagt cagggcagtt tgcaggtcag caaagcggcg aatgtcggta tcagaaactt
    26941 cgcccagctt cgccatgtag tcgttaaagg tcgcctgttc aatgttaaag gctttgtccg
    27001 gacgccagct cggcagcact ttaatggtga aagagccgtc tttggcgatc tctgcgtgat
    27061 gctccagaga atcgatcggg tcatcggtgg tgccgaccat tttcacgttc atctgctgca
    27121 tgatgccgcg cgcggagaag ttatcctgcg ccagcagttc gttacattcg ttccagattt
    27181 catcggcagt tgacggagaa agcaatttgc cagtgatacc aaacggacga cgcagttcga
    27241 ggtgcgtcca gtggtataac gggttgccga tagtgtgcgg aacagtcgcc gcccaggcgt
    27301 caaatttttc acggtcagac gcatcaccgg tgcacagacg ctcggccaca ccgttggtac
    27361 gcatagcgcg ccatttgtag tgatcgccct tcaaccagat gtcatacagg tttttaaaac
    27421 gatagtcttc cgcaatctgc tgcggcggca aatggcaatg gtaatcgaaa atcggctggt
    27481 cttttgcgta gtcgtgatac agacggcggg caaattcagt atctaacagg aaatcttcag
    27541 tcataaacgg agtcattttc gtcttcctct caacgagtga gctagcttgc ttatggtgcg
    27601 atgctgacaa agttatcaca ccaatttcca gagtccgaag atattttcgt gagttagatc
    27661 aataaacgta gttaaaaaaa ttactctcaa agtggtaaat ctcgctgcag gccgcgccag
    27721 tactggcctc gtcgtcgtca ggtaatgtcc ctacaaatat tcgcacattt gtgatggctc
    27781 tcacctttta aagttgtatg acaagttatc tttctgccgt cgcaaatcat aagtcgacgg
    27841 aatgcaaatt gccgattcat tcatttgtta gatgaatcgg gttaaccggt acggaagccg
    27901 aattagcacg aaactttcat ggcaacgttc ggggcgtgcc ggtttttttt cggttacccg
    27961 gtcgtaacta acatcttcag cctctggcgg gatgatggcc gcgcttcctg cggatataac
    28021 aaaacgatga ggttttacat gcgtaaaatt aaagggttac gttggtatat gatcgcactg
    28081 gtgacgctcg gcaccgtgct tggttacctg acgcgtaaca ctgtggcggc agctgcgcca
    28141 actctgatgg aagagttaaa catctccacc caacagtatt cctatatcat cgcagcgtat
    28201 tctgctgctt atacggtcat gcaaccggta gcgggttatg tactggatgt gctgggtacg
    28261 aaaatcggtt atgcaatgtt tgctgtactg tgggccgtgt tctgtggtgc aaccgcgctg
    28321 gcaggtagtt ggggtggcct ggcggttgct cgtggtgcgg tcggtgccgc ggaagccgcg
    28381 atgattccgg cgggtctgaa agccagctcc gaatggttcc cggcaaaaga gcgttccatc
    28441 gcggtaggtt actttaacgt aggttcttcg attggtgcga tgattgcgcc gccgctggtg
    28501 gtatgggcaa tcgtgatgca cagctggcag atggcattta tcatctccgg tgcgttgagc
    28561 tttatctggg cgatggcatg gctgattttc tacaagcatc cgcgcgacca gaaacatctg
    28621 accgatgaag aacgcgacta cattattaat ggtcaggaag cccagcacca ggttgacaca
    28681 gcgaagaaaa tgtccgttgg tcagatcctg cgtaaccgtc agttctgggg tatcgcgctg
    28741 ccgcgcttcc tggcagaacc ggcatggggt acttttaacg cgtggatccc gctgttcatg
    28801 tttaaagttt acggctttaa cctgaaagag atcgcgatgt ttgcctggat gccgatgctg
    28861 tttgctgacc tcggttgtat cctcggtggt tacctgccac cactgttcca gcgttggttt
    28921 ggtgtgaacc tgatcgtttc ccgtaagatg gttgtaacgc tgggtgcggt gctgatgatt
    28981 ggcccgggta tgatcggtct gttcaccaac ccgtatgtcg cgattatgct gctgtgtatc
    29041 ggtggttttg ctcaccaggc actgtctggc gcactgatta cgctctcgtc tgacgtgttc
    29101 ggtcgtaacg aagtggcgac ggcgaacggt ttaaccggga tgtccgcatg gctggcaagt
    29161 acgctgtttg cactggtagt cggtgcactg gctgacacca tcggcttcag cccgctgttc
    29221 gcagttctgg cagtgttcga cctgttaggt gcgctggtta tctggaccgt gttgcagaac
    29281 aaaccagcga ttgaggtggc gcaggaatca cataacgatc ccgcaccgca acattaaggc
    29341 ttcgtgtaag ttttagtttc ccttcaaagc cgccttctca ggcggctttt tcatcactgc
    29401 gagtagagct aaactcgcaa aagtggtata acaaatatag tctgccgtat catgcctgga
    29461 gcgcatatgg aaatcactga accacgccgt ttgtatcaac aacttgccgc tgatctgaaa
    29521 gagcgcatcg aacagggcgt ctatctggtg ggtgataaac tgcctgccga acgctttatt
    29581 gccgatgaaa aaaacgtcag ccgcaccgtg gttcgtgaag ccatcatcat gctggaagtt
    29641 gaaggctatg tcgaagtgcg taaaggttca ggtattcatg tggtttccaa ccagccgcgc
    29701 catcagcagg cggctgacaa taatatggaa ttcgccaatt acggtccgtt tgagttgctc
    29761 caggctcgcc agctcatcga aagtaatatt gccgagttcg cggcaactca ggtcacaaaa
    29821 caggacatca tgaaattgat ggccattcag gagcaggcac gcggcgaaca atgcttccgt
    29881 gattccgagt gggatttgca gttccatatt caggtagccc tggcgacgca gaactccgcc
    29941 ctggcggcta tcgttgaaaa aatgtggacc cagcgtagtc ataacccgta ctggaaaaaa
    30001 ctgcacgaac acattgatgc ccgtaccgtc gataactggt gtgatgacca cgatcaaatc
    30061 ctcaaggcgc tgattcgcaa agatcctcat gctgctaagc tggcaatgtg gcagcacctg
    30121 gaaaacacca agatcatgtt atttaacgaa accagcgacg atttcgagtt caatgccgac
    30181 cgctatctgt tcgccgaaaa cccggtggtg catctcgata cagccaccag cggcagtaaa
    30241 tgaaattcct gtccgacagg cgctacagtg cgcctgtttg tctcgtcggt aagcgaaaag
    30301 tataaagtgt cagcctgtgt aaatcctctc gcctccctcc cctgcattca gcaaaatcag
    30361 actccatgga catgtaattt tgataacgaa caacgttgac ctttgttaca attagattca
    30421 atttgaattt atgcttttga atgctttctt atctcacgat ttaacaggga atagttcagg
    30481 ctgtgttgat gtatcaaacc cgcagaacat accaagacag caataacatt gcggtagtgc
    30541 atcatttaaa accagcgtgg cgttaaccga ttcaccagga ataatgaatg gaacttttga
    30601 cccaattgct gcaagccctg tgggcgcagg attttgaaac cctggccaat ccgtcgatga
    30661 ttggcatgtt gtattttgtc ttgtttgtca ttttgttcct tgaaaacggc ttgcttccgg
    30721 cggccttttt accgggcgac agtttactgg tactggtcgg cgtattgatt gcgaaaggcg
    30781 cgatgggcta tccgcaaacg attctgctgc tgaccgttgc cgccagcctc ggttgctggg
    30841 tcagctatat tcaggggcga tggctgggca atacccgcac cgtacaaaac tggctatctc
    30901 atttacccgc gcattatcat caacgcgcac accatctttt tcataaacac ggtttatcgg
    30961 cgctgttaat tggtcgcttt attgcgtttg tcagaacact gctgccgacg attgccgggt
    31021 tatcagggct gaataacgcg cgctttcagt tcttcaactg gatgagtggt ctgctgtggg
    31081 tgttgatcct gacaactctg ggttacatgc tcggcaaaac accggtattt ttaaagtacg
    31141 aggaccagct gatgtcatgc ctgatgctgc tcccggtggt gctgctggtg tttggcctgg
    31201 caggttctct ggtcgtgtta tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa
    31261 tacctcgcat gtcacttcgc cagctagcct ggtccggcgc tgttttactt ctggtcagca
    31321 cactattgct ggcctggtcc gcggttcgcc agcaagagtc tacgctggcg attcgtgccg
    31381 ttcatcaagg gacaacgatg ccagacggtt tttcaatctg gcatcacctt gacgctcacg
    31441 gcattccttt caaaagtatc acccccaaaa acgacactct gttaattacc tttgactcca
    31501 gcgaccagag cgccgccgca aaagcggtcc tcgacagaac attgccccat ggctacatca
    31561 ttgcgcagca ggacaataac agtcaggcta tgcagtggct gacccggttg cgggataact
    31621 ctcatcgctt cggataactt cctgttatcc gaaacagatc actcactttg gtgatttcac
    31681 cgtaactgtc tatgattaat gaggcggtgg ttaacacgac cgcttcgttg tctggacaat
    31741 cgttcctttg taataggtcc gaatcacaat ggaaggttca agaatgaaat accgcatcgc
    31801 tttagctgtt tctctctttg ctcttagtgc cggtagttat gccactaccc tgtgtcagga
    31861 aaaggagcaa aatatcctta aggagatcag ctatgccgaa aaacaccaaa accagaatcg
    31921 tattgacggt ctgaataaag ccctgagtga agtccgggcc aactgttcag atagccaatt
    31981 gcgtgccgat catcagaaga aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca
    32041 agatttagcc gaggcgaagc aaaaaggcga tgcagataag attgccaaac gcgaacggaa
    32101 actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact aactcacaat
    32161 agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg gaacatctgc
    32221 gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca tctggcgaga
    32281 agtcaaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg aaacagagcc
    32341 gttatcgcct gggtgaaacc ggtgatgcca ttgccaaaca aacccgtgtc gcggcggcgc
    32401 gtgccgatga gtatgtgcgt gaaaatccgt ggacgggcgt gggcattggc gcagcaatcg
    32461 gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc atcacgcgca
    32521 agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca tggttgaaat
    32581 ggtggagaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag cgaatctctt
    32641 tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc ttatgagcct
    32701 gatggtgcta attatctggg cggttgatcc gcaatatcgc ctgaatgcga tgattgccac
    32761 caccgtggtg ttgctgctac tggcactgat tggcggcatc tggacgttac gtaaatcgcg
    32821 taagtctacg ttgctgcgcc acacgcgcca tgagctggca aacgatcggc agctgctcga
    32881 ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc
    32941 agatccagca acaacggctg gatctttccg ccactcgtcg tgaatggctg gaggcaacag
    33001 gcgcttacga tcgtcgctgg aatatgctgt tgagtctgcg ctcctgggcg ctggttggca
    33061 gtagcgtgat ggctatctgg acgattcgcc atcctaatat gctggtccgc tgggccagac
    33121 gcggttttgg tgtatggagc gcctggcgtc tggtgaaaac aaccctcaag caacaacagc
    33181 ttcgcggtta acttcctctc tggccggaga aattccggcc ttattcctca aattttttga
    33241 aaatatttga cagttttcct tgctaacaat cacgcttcgc cacgtttatg attctctcca
    33301 tcgacagcaa cgacgctaat agcgcgccct tgcacaaaaa aacaatcagc agcctgagcg
    33361 gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgtattt
    33421 taatgccgat tctgtttatt accgcaggct ggggaaaaat tactggctac gcgggtaccc
    33481 aacaatatat ggaagcaatg ggcgtccccg gctttatgct gccgctggtg attctgcttg
    33541 agtttggtgg tggtctggca atcctgttcg gtttcctgac acgtaccaca gccctgttta
    33601 ctgcgggctt tacgctgctg acggcatttt tatttcacag caattttgca gaaggcatga
    33661 actcgctgat gtttatgaaa aacctgacta tttctggcgg attcctgctg ctggcaatta
    33721 ccggtccagg cgcattcagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta
    33781 tacttaacga taaaaaacga gggggaagct cctcgttttt gctattggag gatagaaaaa
    33841 tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa tccaccggcg
    33901 gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcgccag
    33961 gccccactgg caaaggcggt tttgccgcag agaaagatcg ttatcatctc tatgtttcac
    34021 tcgcctgccc gtgggcacac cgcacgctga tcatgcgtaa gctcaaagga ctggaaccgt
    34081 ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca
    34141 gttttccggg agcaagcggc gacacgctct atcaacatga atttctgtat cagctttatc
    34201 tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg gacaaaaaaa
    34261 accacaccat cgtcagcaac gaatcagcag aaatcatacg catgttcaat accgcgtttg
    34321 atgcgctggg tgcgaaagct ggagattact acccaccagc cctgcaaccg aaaattgacg
    34381 aacttaacgg ctggatttac gacaccatta acaatggcgt gtataaagcc ggttttgcca
    34441 ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg
    34501 aacagatttt aggtcagcac cgttacctga ccggcaacca gctaaccgaa gccgatattc
    34561 gcctgtggac cacgctggtg cgttttgatc cagtgtatgt gacccacttc aagtgtgata
    34621 agcaccgcat cagcgattac ctgaatctgt atggcttcct gcgcgatatc taccagatgc
    34681 cgggaattgc cgaaacagtc aatttcgatc acatccgtaa tcattacttc cgcagccata
    34741 agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc gatgaaccgc
    34801 atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct ctgataatta
    34861 gctaagtacg tgttgttcat gtcggatgcg gcgtaaacgc cttatcaaac ctacaaaaca
    34921 tttatattca atatgttgca atgaccgcgc aggccagata agcgtagcgc atctggcaat
    34981 tttgcgtttg cccttatcct tcacagcgcc ttattaattc atcgcgataa tatttactgc
    35041 catttcattc acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg
    35101 aggcaaaaat ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg
    35161 cgcggcgcaa agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg
    35221 ggctactgga taaaatgtta ggctggcaac gtgccggtgg cgaaggcatc ctgacgacaa
    35281 tctacggtat tctggtgttt ttaccatggt gggcagttca gttccgccgc ctgcacgaca
    35341 ccgaccgctc ggcgtggtgg gcactgctat tcttaatccc gtttatcggc tggcttatta
    35401 tcatcgtctt taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga
    35461 aactggaaca agaataataa ttaagcacct tgtaaaaggt gcttaatcga ttattttccg
    35521 ctaaaaagtt tgggaatttc ccgcagacac caggatttcg cctcccccat actgtcacga
    35581 cgccaggcca taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc
    35641 ccttccgcaa tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc
    35701 agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc
    35761 aactgtacgg tcaacaccgg gcgctcacga gcggtatccg ccaccgcaat tccacgatat
    35821 ttcacgcgcg tcacttcaga taacggttcc ggctcctgat gaatcgggtg atccggcgcg
    35881 gcaacataga cgttcattaa ggtatagagt ttgcgcgagt tgatctccga cgaggaacga
    35941 aaatgcatat ccggcgcgat aacgatatcc gcccgcccct gctccagccg ttcccacgcc
    36001 cccgccagca cttcggtgat aattgccagt tgggtattgg cttttgccgc cagtttgtcg
    36061 attaacggga aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt
    36121 tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa
    36181 acgcgccctc gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag
    36241 cggtcaaaca gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg
    36301 gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca
    36361 tccataaccc gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc
    36421 aggaaatttg aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac
    36481 cataaaagga agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg
    36541 tggacaagca gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt
    36601 cgacccgaaa ttgttaggct atgcctccct gcgcgtgctt aaccaggaag tgctggcccc
    36661 aggtgccgcc tttcagccac gaacctatcc caaagtcgat attttaaatg tgattctgga
    36721 tggagaagct gagtatcgcg acagcgaagg caatcatgtt caggccagca ctggtgaggt
    36781 gttgctgctc tctacccagc cgggtgtcag ctatagcgaa cacaatctca gcaaagacaa
    36841 accgttaacg cgaatgcagc tttggctgga tgcctgcccg cagcgagaga atccgctgat
    36901 tcaaaagctg gcgcttaata tgagcaagca gcaattaatc gcctcgccag aaggtgggat
    36961 gggaagcctg caattgcgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga
    37021 aagtgcgaat ttccagttgc atgggccacg cgcgtatttg caatcgattc acgggaaatt
    37081 tcatgcgctt acgcatcatg aagagaaagc ggcgctaacc tgcggtgatg gggcgtttat
    37141 tcgtgacgag gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga
    37201 tttgcctgtc tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc
    37261 gccagccagt gaagcccgtg gtggcgaaag aacctgctcg taccgccaaa aattttggct
    37321 atgaagagat gttgagcgaa ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg
    37381 aggatgaagc taccgcgtaa agacaggttg ccggatgcgg cgcaaacgcc ttatccggca
    37441 caatacccaa aaccgggcgt ttatctggcc ttgctcgcca taatctcgat aatctgccga
    37501 tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga ctgctcaaca
    37561 tcatgcgcca caatcccttc attgccggtc acggcggtat catccagcgc cattaacacc
    37621 gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt cgatgcacca
    37681 tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga gatggtttca
    37741 taacgcccat ccaccagcca tgccatcccg gcagcggccc ccattgctgc ggtcgttgcg
    37801 gcacacagcg cagacaaacg cggtaactgg ttatggatgt aaatcgcgct caaatgcgaa
    37861 agcatcagcg cacgcgccag ccgttcatca tccgctccga agtgttcagc taccaccacc
    37921 acgggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat tgccggaagc
    37981 gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag
    38041 agatctttcg ccagcaaacc gcgcgcgcac tgtttttcca gcgtcgcacc aatatgcagc
    38101 ccccagttac cgctcaaacc ttcctgcgat aacgcacaat ttaacttcgc agaatcgaga
    38161 ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc agccagcgtc
    38221 gtcctggaaa gcaccgtcag cggcgactct tgctcgccct ctgccacaca cgcctgctgg
    38281 gtaaacacca caccattgtg cgtctcaata tgcacaatgt tggtatgccc gccgacgatg
    38341 gtgacacacg cccatttctc accgttccag actttggcgc gtgagaagag gatttcatcg
    38401 caaggttcct ggatcttaac tgagactttc cccgccgcca gcagtgcttt ggcatcggaa
    38461 attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttagcatt tccacctaac
    38521 gctcccagcg ccgccgcaat tggcagcccc accattcctg taccgggaac ggtgacaccc
    38581 agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc
    38641 agttcagccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acaccccagc
    38701 gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg atttaaagtc
    38761 gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacgga
    38821 gaaacacaaa gcaacaaacc agtgacgata atcaggtaca aagacatccc tttgtatttg
    38881 tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa
    38941 atcgggctac agatagaggt gaagctcaac accggggcgt tcagtacgat ggcgctccag
    39001 gccagcaaaa tggcgaaaat catgatgccg cgctgaacga gattttcgtt aatcttctcg
    39061 gcaggtatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gcgaaagcct
    39121 aaatagacgc caaagaatgc agtcattaca gcaaagatat tgagaatgac gctgaccact
    39181 ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgcggaaata
    39241 ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa cgacacggcg
    39301 tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat tcattgcccg caatgcttta
    39361 tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat cgggcttaac
    39421 gtctggataa atagaatcga cgtcagggta aacggcagcg taataatggc gtttttcatc
    39481 agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc caccatcgac
    39541 acgcccagcg ccgcgaccac cagcagcttg gtcagcacca tgccggtcga aattttgaat
    39601 aacaatttct cgccgcgtga ggagatcgcc accagaatgc aaatcagtac cagaccataa
    39661 aagggactgt ctgacagcaa cccttccgtc acgccgaagg tatgcaggta ggaagcacta
    39721 tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac gaaatagagt
    39781 gcacctaaca ggatgcccca gtttttacct aaataaccgc tgatgacgct cgggtaatct
    39841 ttacattctg gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc
    39901 gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc
    39961 gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac ccagccagtg
    40021 tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc agcacgaccc
    40081 gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat aatttctcgc
    40141 tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat atgcggtcta
    40201 cgtttttgca ggcgagttat tagaataaag aaatgatacg cgtgagatca gagaaaatct
    40261 tcacaatcaa gacttttaaa tagcaattgt gggtaataat tttctattaa attgagttaa
    40321 tagaaataat tattctctat ttgcggcaga tcacaataaa aaagggtgca cattttgtgc
    40381 acccaaggat gatagctgac agcaatgtca gccgcagacc actttaatag ccagtcctcc
    40441 gcgtgatgtt tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat
    40501 cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt
    40561 tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc
    40621 aaccggatcg caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac
    40681 ctgcgccgga ctgccaccca gtagttcagt taaccctgcc gctgccattg aacaggccac
    40741 gccaatctcc ccctgacagc cgacttccgc gccagagatg gaggcgttca ttttatacag
    40801 cgcgccaatt gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg
    40861 acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg
    40921 tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt
    40981 gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctggaaa ccagttgacg
    41041 acgcagcgct acggcacggc gcggcacatt gagcggacca ggcagcacgc cttcagtatt
    41101 cattccacgt tcaataccgt catgcatcac ttgccagata cgggcaaacc ctgcgtcaat
    41161 ttccgcttta ctgcgcagcg ccagctcgtt gtgcatcatc aggccagata tagacaggcc
    41221 gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacgggtgt
    41281 ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga
    41341 gtaataggtt ttgcttaata gctcttcctg tcccttccag gcagtgatcc gcattccgtt
    41401 ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac
    41461 aatatgcgcg ccagaagcca ccggcaaccg cccgctgcgc gtcactaact ctataaatgc
    41521 agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca tgatgatggc
    41581 gacatccgtg gcatggcctt tgcccgtcag tgacaacgac ccgtacagat cgaccacaat
    41641 atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc
    41701 attcattggc cccacagtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa
    41761 tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt
    41821 gcgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatggcttc aatttccagc
    41881 ttcacatctt tcggcaaacg cgcgacctgc acacagctcc gggtcgggta ggtcgcctga
    41941 tgttcatcga agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg
    42001 atgaacacgg tcatcttgat gatatcgccc acactcagcc cggcagcaac cacgatcgct
    42061 ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc
    42121 tgtgggcaaa ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga
    42181 acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct
    42241 ccggcgtcag agcgcctggg taaaggtacg tgaaataaca tcctgttgct gttcacgggt
    42301 cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc
    42361 cgggtgctcg atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg
    42421 accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt
    42481 tttacgtact ggatcttctt tgcccagcgc cgcagggacg atggagaagg tgtacgagat
    42541 cccatctttg gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc
    42601 tttgcgatca cgaccgtgca tcgggttagc gcccggcgcg aacggtgttc cggcgcggcg
    42661 accgtccggc gtgttcccgg ttttctgacc gtacaccacg ttagaggtga tagtcagaat
    42721 cgattgggtc ggcacagcat tgcgataggt tggcaaagct ttaattttct tcataaagcg
    42781 ttcaaccaga tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccat actgcggata
    42841 ttcgccgtca atctcgaaat ccaccgccag gccgttttca tcacggattg gtttcacacg
    42901 agcatatttg atggcagaca gggagtccgt tgccaccgac aggcccgcga tgccgcacgc
    42961 catggtgcga tagacatcac gatcatgtag cgccatcagc gaagcttcgt agctgtactt
    43021 gtcgtgcatg tagtgaatga tattcagcgc gctgatgtac tgtaccgcca gccagtccat
    43081 gaagtggtcg aggctatcca tcactttgtc gtagtccagc acgtcatcca tcaacggagc
    43141 cgttttcggg ccaacctgaa tcttcagctt ctcgtccatt ccgccgttga ttgcgtacag
    43201 tagcgttttc gccaggttag cgcgcgcacc gaagaactgc atctgcttac caatcaccat
    43261 tgggctgacg caacaggcaa tcgcgtaatc gtcgctattg aagtcagtac gcatcagatc
    43321 gtcgttttca tactgcaaag aggatgtgac gatagacacc tgcgcggcat attttttgaa
    43381 ggcaatcggc aaggcttccg accacagaat agtcaggtta ggttccggtg caggccccat
    43441 agtgtgcagg gtgtgtaaat agcggaagga gtttttggtc accagggttc gaccgtccaa
    43501 ccccatcccg ccgatcactt ccgtcgccca gatcgggtcg ccggagaaca gcgaatcaaa
    43561 ttccggcgta cgcaagaagc gcaccatacg gatcttcatg atgaagtggt cgatcagttc
    43621 ctgtgcctgc tgctcattga gtacgcctgc tttaaagtcg cgctcaatgt agatatcgag
    43681 gaacgatgcc gtgcgcccca gcgacatcgc gccgccattt tgcgatttca ctgccgccag
    43741 gtaagcgaag tagagccact gcacggcttc ttgtgcattc tgcgctggac gagagatatc
    43801 aaagccatat ttcgccgcca tttcctgaat ctgtaacagc gcacggcgat gttctgccag
    43861 ttcctcacgc agacgaatgg tggcttcgag atcttcgcct ttttccagac gggactggag
    43921 atcggcaaac tgcagttcgc gttcacgtac cagataactg atgccgtaca gtgccacacg
    43981 gcgatagtca ccgatgatac gcccacgacc ataaccatct ggtaaaccgg tcagtacacc
    44041 agatttacgg cagcgcagca tatccggtga gtaagtatca aatacgccct gattatgggt
    44101 tttacgcaga tcggtaaaga tgtattcaaa ttcgctatcc atttcacgac catacgcgtg
    44161 gaatgaactt ctaatcatat taatgccgcc gaacggatgc agcgcacgct ttaatggcgc
    44221 atccgtttgc agacccacta ttttttccag cggctggtta atatatcccg cgtcatgagc
    44281 ggtaattgtg gtggcaatat tggtatcgaa atccaatggc gcgtgagtcg cattttcgat
    44341 acggatgcct tccatgactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag
    44401 aaaagattca tcgccttcat aaggtgtata gttatgttga ataaaatcgc ggacattaat
    44461 ttcgtttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct
    44521 ggtatcaata tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc
    44581 aaattctgcg ggcgcgttca ctttgcctaa atgaatggca tccaaagcaa tcattttttc
    44641 ttcgttagtc ggaattacgg cgcaaatgac acgcgcattt tcactggaaa caattcgctc
    44701 tccaaaagag ttagagcgat tattcatttc tgtatcaatt tctacgccta atacagctaa
    44761 atgttccatg accagacgac gaattaaact tgaattctct cctattccgc cggtgaatat
    44821 aattccatcc aggcgatgta atgaagcagc gtgtccggca atatgacggg caattcggtg
    44881 aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag
    44941 aacacgtaaa tccgaagaaa ggccggaaat acctaataat cccgactctt tattcactac
    45001 gcgttccagg tcccccaggc tctggttggt ttggctggcg acccaggaca tcgccccgaa
    45061 gtcgacatca ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga
    45121 agtgtcaaca ctctgaccgt tgcgaaccgc gcagattgat gcgccattgc caagatgcgc
    45181 cacaaccagg ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac
    45241 atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt
    45301 ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa
    45361 taccgccacc tgagttacgc ccgggaataa ttgctgcgcc gattcaatac cacttaaatt
    45421 ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc
    45481 ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa
    45541 agccacactg tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc
    45601 ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt
    45661 ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc tggcattgag
    45721 tacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt
    45781 catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg
    45841 tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag
    45901 acggcgcttt acggatggca tacatcggca acaggcacag cagtgatgcg ataatcggtg
    45961 cgcccatggc ttcaatcaag tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg
    46021 agcccataat gaaaatcatg ctgatagtat tcagtttacc cagcgacact ttggttttgt
    46081 cacctttata accgaacttc agaatcaaac cattcagccc ttccagcgtc cccagatagt
    46141 gaccgaagaa agatttgaaa atagccacga gtgcgatgat ggaagcggca tattccagtg
    46201 taatcgcaaa cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa
    46261 gcactggaat attctgcgct ttcgcttcag ccatattcgc cggagacagc gtaaacaggc
    46321 agctaaaggc aaagaacatc accactgcaa ccatcagcat actggcgcga gagatgattt
    46381 gtgaacattt acgctcggtg aagtcgcgac caaaatcttt ctcgtactct tcacgtttag
    46441 aaaccacgaa ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc
    46501 ccagccagac ggtgatcagg ataccgtcat gaccagttaa cgacagcgaa ccgaggtcaa
    46561 cctggtcgat aactgcagag ttccagtaag ggatcagcga aagagaaatc agcaccaggc
    46621 tggcgataaa cggccatacc agatagctca tcactttaac catcagatcc ttaccaaacc
    46681 agatgacgaa agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg
    46741 caaagccgag ctggttttcc cagaacgtca taaaggtgtt ggtaatggta acgccataaa
    46801 tccacaacag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt
    46861 taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac
    46921 acagacgcgc cagtgcccgg tggcaataaa acgcgatggg gtatgctaat accaacatca
    46981 gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc
    47041 cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt
    47101 gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct caacgaatta
    47161 attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg
    47221 aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg
    47281 cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa
    47341 tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa
    47401 cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg
    47461 tagttcggtg cgtagttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt
    47521 cagactgtac gccaataaca cggatggtcg ggttgataga tttaattgcc accgcaatac
    47581 cagcaattaa accgccgccg ccaattggca caatcacgtt atcgacatca tagagatctt
    47641 ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg
    47701 ggataaaaat acggccttcc atttcgacaa tttcgctcac tttagcaata gtgtcgttga
    47761 agttatcacc atgcaaaacg acttctgcgg agtagtcgca cgttgccgcg actttggatt
    47821 tcggcgcacc ttttggcatc accactttac cgtcaatacc cagcatcgcg caggagagcg
    47881 aaaccccttg cgcatggttg cccgcagagc aggccactac tcctttacgt ttttccgcat
    47941 cagtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaacca gtacgctgca
    48001 tgttttcgaa tttgaggaat atttcacctt tgcaacgttc actaaaatag ttggagcgag
    48061 gcatacctgt tttataaatt cgcccagcca gtcgttgttt tgcttcaatg atgtcatcaa
    48121 tagcaaccgg gagatcgtat gtaatatgca ttataaaacc tcttagcctg aattaaatat
    48181 gtaggtaacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaattaa
    48241 aacaaataac taatcaactt caattaattg ccttcgtcta cacccattat aagatgaata
    48301 ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca
    48361 taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt
    48421 agaaccaaaa ggtgatgtca tatcacaagg aattacagtt aagaaatcag cattgagaac
    48481 aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt
    48541 tctttgtaac gtagtaagta gttcgctgta gtaccccata ttggtttgtg gcaacaccca
    48601 ctgttcgttc ttcaacgact ccagcgtggc ggtgccggtg catgttcggg acttactggc
    48661 taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc
    48721 tgcgcttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga
    48781 agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt
    48841 gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac
    48901 caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat
    48961 ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt
    49021 ccgcaccact aactccaccc caaaataatc ttcaatatcg ttaatgattt tactgacggc
    49081 cggttgagtt aaccctaact cttttgcagc cgagccgata gaaccacttc taataacttc
    49141 ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt
    49201 taattacctc attgacggta tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc
    49261 actcacaaat taactttcaa tactttccat agcatcgtta ccccaattaa atacctttaa
    49321 gtatcaacaa gttaaagtat gaaaatcagc ataaaccctg atttttgtca aaaaaaatga
    49381 cggggataac catattttat ggcgataaca tcattcttta tgtggttaat acaaaaaagg
    49441 ggctgagact atatttcaag caattaccgg ataattacca ggcaaaattt gagt
//
LOCUS       DAJOTB010000017        47337 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJOTB010000017 DAJOTB010000000
VERSION     DAJOTB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.017"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            77..997
                     /locus_tag="OHN28_000986"
     CDS             77..997
                     /locus_tag="OHN28_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2296424.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            1068..1706
                     /locus_tag="OHN28_000987"
     CDS             1068..1706
                     /locus_tag="OHN28_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2296425.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1997..2725
                     /gene="mpaA"
                     /locus_tag="OHN28_000988"
     CDS             1997..2725
                     /gene="mpaA"
                     /locus_tag="OHN28_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCQ2296426.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2700..3665)
                     /gene="ycjG"
                     /locus_tag="OHN28_000989"
     CDS             complement(2700..3665)
                     /gene="ycjG"
                     /locus_tag="OHN28_000989"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCQ2296427.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3784..4290
                     /gene="tpx"
                     /locus_tag="OHN28_000990"
     CDS             3784..4290
                     /gene="tpx"
                     /locus_tag="OHN28_000990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCQ2296428.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(4334..5875)
                     /gene="tyrR"
                     /locus_tag="OHN28_000991"
     CDS             complement(4334..5875)
                     /gene="tyrR"
                     /locus_tag="OHN28_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCQ2296429.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6023..7084)
                     /locus_tag="OHN28_000992"
     CDS             complement(6023..7084)
                     /locus_tag="OHN28_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCQ2296430.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7081..8478)
                     /locus_tag="OHN28_000993"
     CDS             complement(7081..8478)
                     /locus_tag="OHN28_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCQ2296431.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8634..9632
                     /locus_tag="OHN28_000994"
     CDS             8634..9632
                     /locus_tag="OHN28_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2296432.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(9683..10588)
                     /gene="ompG"
                     /locus_tag="OHN28_000995"
     CDS             complement(9683..10588)
                     /gene="ompG"
                     /locus_tag="OHN28_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCQ2296433.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNNWHFNIGEMYEIENVEGYV
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSLGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10633..11715)
                     /locus_tag="OHN28_000996"
     CDS             complement(10633..11715)
                     /locus_tag="OHN28_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2296434.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     IQKIAKEMIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(11729..12388)
                     /gene="pgmB"
                     /locus_tag="OHN28_000997"
     CDS             complement(11729..12388)
                     /gene="pgmB"
                     /locus_tag="OHN28_000997"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCQ2296435.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKLDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(12385..14652)
                     /gene="ycjT"
                     /locus_tag="OHN28_000998"
     CDS             complement(12385..14652)
                     /gene="ycjT"
                     /locus_tag="OHN28_000998"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCQ2296436.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGRDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNERLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(14649..15704)
                     /locus_tag="OHN28_000999"
     CDS             complement(14649..15704)
                     /locus_tag="OHN28_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2296437.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASLWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFAFDTQQLREQVTNGVLGEIYVTTARA
                     LRRSGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(15714..16502)
                     /locus_tag="OHN28_001000"
     CDS             complement(15714..16502)
                     /locus_tag="OHN28_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2296438.1"
                     /translation="MKIGTQNQAFFPGNILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDHRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            complement(16520..17572)
                     /locus_tag="OHN28_001001"
     CDS             complement(16520..17572)
                     /locus_tag="OHN28_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCQ2296439.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17603..18445)
                     /locus_tag="OHN28_001002"
     CDS             complement(17603..18445)
                     /locus_tag="OHN28_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCQ2296440.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYIFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18432..19313)
                     /locus_tag="OHN28_001003"
     CDS             complement(18432..19313)
                     /locus_tag="OHN28_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCQ2296441.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(19334..20626)
                     /locus_tag="OHN28_001004"
     CDS             complement(19334..20626)
                     /locus_tag="OHN28_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2296442.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQR"
     gene            complement(20640..22319)
                     /gene="ycjM"
                     /locus_tag="OHN28_001005"
     CDS             complement(20640..22319)
                     /gene="ycjM"
                     /locus_tag="OHN28_001005"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCQ2296443.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(22530..22844)
                     /gene="pspE"
                     /locus_tag="OHN28_001006"
     CDS             complement(22530..22844)
                     /gene="pspE"
                     /locus_tag="OHN28_001006"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCQ2296444.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(22919..23140)
                     /gene="pspD"
                     /locus_tag="OHN28_001007"
     CDS             complement(22919..23140)
                     /gene="pspD"
                     /locus_tag="OHN28_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCQ2296445.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(23149..23508)
                     /gene="pspC"
                     /locus_tag="OHN28_001008"
     CDS             complement(23149..23508)
                     /gene="pspC"
                     /locus_tag="OHN28_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCQ2296446.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(23508..23732)
                     /gene="pspB"
                     /locus_tag="OHN28_001009"
     CDS             complement(23508..23732)
                     /gene="pspB"
                     /locus_tag="OHN28_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCQ2296447.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(23786..24454)
                     /gene="pspA"
                     /locus_tag="OHN28_001010"
     CDS             complement(23786..24454)
                     /gene="pspA"
                     /locus_tag="OHN28_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCQ2296448.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            24621..25598
                     /gene="pspF"
                     /locus_tag="OHN28_001011"
     CDS             24621..25598
                     /gene="pspF"
                     /locus_tag="OHN28_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCQ2296449.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            25752..25889
                     /locus_tag="OHN28_001012"
                     /pseudo
     CDS             25752..25889
                     /locus_tag="OHN28_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            26023..26268
                     /locus_tag="OHN28_001013"
     CDS             26023..26268
                     /locus_tag="OHN28_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCQ2296450.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            26581..28224
                     /gene="sapA"
                     /locus_tag="OHN28_001014"
     CDS             26581..28224
                     /gene="sapA"
                     /locus_tag="OHN28_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCQ2296451.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            28221..29186
                     /gene="sapB"
                     /locus_tag="OHN28_001015"
     CDS             28221..29186
                     /gene="sapB"
                     /locus_tag="OHN28_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCQ2296452.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            29173..30063
                     /gene="sapC"
                     /locus_tag="OHN28_001016"
     CDS             29173..30063
                     /gene="sapC"
                     /locus_tag="OHN28_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCQ2296453.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            30063..31055
                     /gene="sapD"
                     /locus_tag="OHN28_001017"
     CDS             30063..31055
                     /gene="sapD"
                     /locus_tag="OHN28_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCQ2296454.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            31057..31863
                     /gene="sapF"
                     /locus_tag="OHN28_001018"
     CDS             31057..31863
                     /gene="sapF"
                     /locus_tag="OHN28_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCQ2296455.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(31911..33074)
                     /locus_tag="OHN28_001019"
     CDS             complement(31911..33074)
                     /locus_tag="OHN28_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCQ2296456.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(33083..34456)
                     /locus_tag="OHN28_001020"
     CDS             complement(33083..34456)
                     /locus_tag="OHN28_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCQ2296457.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(34460..37567)
                     /locus_tag="OHN28_001021"
     CDS             complement(34460..37567)
                     /locus_tag="OHN28_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCQ2296458.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRTQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(37567..38688)
                     /locus_tag="OHN28_001022"
     CDS             complement(37567..38688)
                     /locus_tag="OHN28_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCQ2296459.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(38837..39403)
                     /locus_tag="OHN28_001023"
     CDS             complement(38837..39403)
                     /locus_tag="OHN28_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCQ2296460.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            39881..40669
                     /gene="fabI"
                     /locus_tag="OHN28_001024"
     CDS             39881..40669
                     /gene="fabI"
                     /locus_tag="OHN28_001024"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCQ2296461.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            40814..41941
                     /locus_tag="OHN28_001025"
     CDS             40814..41941
                     /locus_tag="OHN28_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCQ2296462.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            42009..43943
                     /gene="rnb"
                     /locus_tag="OHN28_001026"
     CDS             42009..43943
                     /gene="rnb"
                     /locus_tag="OHN28_001026"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCQ2296463.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(44020..44123)
                     /locus_tag="OHN28_001027"
                     /pseudo
     CDS             complement(44020..44123)
                     /locus_tag="OHN28_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44177..46162
                     /gene="pdeR"
                     /locus_tag="OHN28_001028"
     CDS             44177..46162
                     /gene="pdeR"
                     /locus_tag="OHN28_001028"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2296464.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            46310..46483
                     /locus_tag="OHN28_001029"
     CDS             46310..46483
                     /locus_tag="OHN28_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296465.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            46572..47321
                     /locus_tag="OHN28_001030"
     CDS             46572..47321
                     /locus_tag="OHN28_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2296466.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
BASE COUNT        11600 a        12569 c        11516 g        11652 t
ORIGIN      
        1 taatcaactt aatgatcaat atctatagtg atatcaccaa ccgttaaaac gaaccgacaa
       61 ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca ataatccaac
      121 catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc
      181 ggcaattgtg gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc
      241 cgaaaaactg gcggaaaaag gatttgtcac tattgcttat gacgcatctt atcaaggtga
      301 aagcggcggc gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc
      361 cgtgattgat tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg
      421 aatttgtgcc ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc
      481 catcggtact gttagtgcgg tcaatattgg ttcgatgttc cgtaatggtt gggaaaacaa
      541 tgtgaaatct attgatgccc taccatacgt tgacgcgggt tcaaacgccc gaaccagcga
      601 tattagcagc ggagaatacg ccactatgcc actggcacca atgaaggagt ccgatgcgcc
      661 gaacgaagaa ctgcgtcagg cctgggaata ttaccacacc cctcgcgcgc agtatccaac
      721 agcaccgggt tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca
      781 tatggcggaa gtgtacctga ctcagccgat acagattgtg gcgggcagcc tggctgggag
      841 caaatggatg agtgacgacc tgtacgatcg agcatcaagc caggataaac gctatcacat
      901 tgttgaaggc gcaaaccata tggatcttta tgatggcaaa gtctatgtgg ctgaagtaat
      961 ttcagtatta gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgtaat
     1021 agtgatatgg cctggcatat tgctgccaat aatctgggaa aagtttcatg aaaaatgtac
     1081 tgattcttgg tgctggcggt cagatagccc gccatgtgat taatcaactg gcagataaac
     1141 aaaccattaa acaaacttta tttgcccgtc agccagcaaa aatccacaaa ccctatccaa
     1201 ctaatagcaa gattattatg ggggatgtac tgaatcatgc agcactgaag caggccatgc
     1261 agggacagga tgttgtgtat gccaacctta cgggggaaga tttagatatt caggcaaatt
     1321 cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg
     1381 gtatttatga tgaagttcca ggaaaatttg gcgaatggaa taatgcagtt attggtgaac
     1441 ccctaaaacc attccgcagg gctgcggatg caattgaggc ttctggactg gaatatacaa
     1501 ttcttcgccc tgcctggctg actgatgaag atattattga ttatgagctt acctcacgca
     1561 atgaaccatt caaaggtacg atcgtatcac gtaagagtgt cgcagcatta atcacagata
     1621 tcatcgataa acctgagaaa cacattggtg agaatatagg catcaatcaa cccgggactg
     1681 atggtgataa gcccttcttc atgtaaatca attatatagc taccatcgta ctgtactatg
     1741 tgcagtacga tttattaaac ttattaaggt gaactgatca atcagatgcg attaacatgt
     1801 gtagaaccag taagaataaa atacgccatg tatataacat tacgacaatt ctaatatctg
     1861 cacatcaatg aaaatgcaaa caacttccgg caaaaaagtg atacaagatc gatgacatag
     1921 cgttgaagaa aaggatttga taatccgcaa aatttgcaca acactgcctg taatacttcc
     1981 tgaaccagag acgatcatga ccgtaactcg cccacgcgcc gaacgcggcg catttccgcc
     2041 cggaacagaa cattacggac gttcattatt gggcgcgccg ttaatctggt ttccggcccc
     2101 tgcagccagt cacgaaagtg gtttgattct ggctggcact cacggtgatg aaaactcttc
     2161 gatcgtcacg ctctcctgtg ctctgcggac tttgacacct tctttgcgcc gccatcatgt
     2221 ggtgctgtgt gtgaatcctg acggctgcca gttagggtta cgggccaatg ctaatggtgt
     2281 ggatttaaac cgaaactttc cggcagcgaa ctggaaggaa ggtgaaacag tttatcgctg
     2341 gaacagcgcc gctgaagaac gtgatgttgt attactgaca ggtgacaaac ctggctccga
     2401 acctgaaacc caggcgctgt gccagcttat acaccgcatt caacctgcct gggtggtctc
     2461 attccacgat ccgctggcct gtattgaaga tcccagacac agcgaattag gtgaatggct
     2521 ggcccaggcg tttgaattgc ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt
     2581 cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc gcagagtttc cacccatctc
     2641 ctccgacgaa gccagcgaaa aatatctctt tgcgatggca aacttgctgc gctggcatcc
     2701 taaagatgca attcgcccgt cgtgaactga agcgccggtt ccacatctac cgccagccag
     2761 gtcggtccgt caagatcggc gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg
     2821 gcacgagagg tacacaacat gcagcccagc atcagacgaa aaccttgtgc acgcgcttca
     2881 gtcgccagcg ccagcgcttc cgtcagacca ccggttttat caagcttaat gttaaccatc
     2941 tcatagcgcc ctttcagcgc cttcagattg ctacgagtat gacaactttc atcagcacaa
     3001 atcggtaacg gatgaataaa attctccagc gccgcatcgt cctgcgcagg aagcggttgt
     3061 tcaagcatcg caacgcctaa atccgccaat agctggcaac gcgccgccaa cccttctgca
     3121 cgccaggatt catttgcatc aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc
     3181 accatccgct cactgataag atggttatcc agcttcactt tcagtaattt cgcgcctgcc
     3241 tgccagagtg ttgatgcact attggccatc tgatcaggcg taccgatgac tatcgtctgt
     3301 gccgtggtga ctgtctcggg aagcgttatg ccgatcaaat cagccagcga ttgctgctgt
     3361 tttcgcgccg ccagatccca caatgcacaa tccagcgcat tacgtgctgc gccggcaggg
     3421 aggatttttt gcaactcctc ccgtgtcagc cctttctcta gttgcggcac gacgctcata
     3481 atttgcgcca ttaccgaggc atcgctttcc ccataacgcg gatacggcgt gcattcaccg
     3541 gtgcctttga taccctcttc ttccagttca accaccacca cgcgcgcttc actgcgactt
     3601 ccccgagcaa tcacaaacgg ggtatgtaag ggccaggcct cctcaaatac cttaacggtt
     3661 ctcatcttta ctccttgttg tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg
     3721 tgttattgac agttagcata aactaggtgt gacgttaact atatgtaaac aggaaagata
     3781 atcatgtcac aaaccgttca tttccagggc aacccggtta cagtcgccaa ttccatcccg
     3841 caggcgggta gcaaagcgca gacttttact ctcgtggcaa aagatctgtc tgacgtcacc
     3901 ctcggtcagt ttgcgggtaa acgcaaagtg ctgaacattt tcccgagtat tgataccggt
     3961 gtttgcgccg catcagtacg taagtttaac caactggcaa ccgagatcga caacaccgtt
     4021 gtgctgtgta tctctgccga tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt
     4081 ctgaacaacg ttatcaccct ctccactttc cgtaacgctg aattcctgca agcctacggt
     4141 gtggcaattg ctgatggccc actgaaaggt ctggcagcgc gtgccgttgt ggttattgat
     4201 gaaaatgaca atgtgatttt cagccagctg gtggatgaaa tcaccaccga gccggattac
     4261 gaagcagctc tggctgtact gaaagcataa gttaatatgc ctgatggtat tgcaccatca
     4321 ggcatattca cgcttactct tcgttcttct tctgactcag accatattcc cgcaacttat
     4381 tggcaatcgc ggtatgtgaa acgccgagac gttttgccag tttgcgcgtg ctgggataat
     4441 tgcgataaag ctgggttaat accgagcgtt caaaacggct ggtgatttcg tccagcgaac
     4501 cttccatcgc atcttcgccc acggctaccg ttgcggcgtc ataatccggc aacaaaatat
     4561 cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc acgatagata gcgttcttta
     4621 actgccgcac atttcccggc cacgcataac gcgtaagtac agtattcaga tcagcggcca
     4681 gtttcggacg cggcacgccc tgctcgtcgg caaagcgggc gacgaacagc tcggttaacg
     4741 gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt gagcgtcaac acgttcagac
     4801 gataatagag atcttcacgg aacacgcctt tttgcaccag ttcgaccaga ttcttctgcg
     4861 tagcgcaaat cacccgcaca tcgacatgca cctcatggtc ttcgccaacc cgacggaagg
     4921 tgccatcatt aaggaaacgc agtaatttcg cctgcatccg tggtgacatc tcacctattt
     4981 catccaacag caccgaacca ccgttcgcct gctcaaagaa ccctttcttc ccttccggag
     5041 catgaccaaa cagttcactc tcgaccgcat cttccggtat agacgcacag ttcagcgcca
     5101 ggtaaggttt gctcgctctg gggcttgcct gatgacaggc gtaggcaaag agatcttttc
     5161 cggtgccggt atcacccgta atcagcagtg gcgcgcttag catcgccagt ttctgcgcct
     5221 gttcgacaac atgcttcatt ttcgggctga cagcgacaat ttgactgaag gcgctgacat
     5281 cctgggcggc gacattttgc aactgacggc ccatacgaat cgttgatcgc agcatcacca
     5341 ccgcaccggt caggacgtgt tgatcatttt catcctgaag ataaacaggc gtaatctcca
     5401 tcaggaaatt ctgcccatta ataacgacat gctcgttatg cgaatcttgc ggttcgcttt
     5461 ccagccagcg taaaaaatta aagccgttaa tcaattgcgc ggcggtatgg ttgcgcaggc
     5521 gatccaattt ttgcccaaaa agctgacagc tcgccgggtt cgccatatcc actttgcttt
     5581 tcatatcgac agagagcaca ggttcaggca acgcctccag taacgcgctc aacgccagat
     5641 gctcacgttc ggaaggcatc cacgggacag tacgcacatc ggtaacacct gcaatacggc
     5701 gtatttcggc catcagactg ctgaaactct caaactccag ttcagcaaaa ttgaggtaga
     5761 ttcgcccaat ggggtcaatc tcaataccgc gtaaatcaat gcctcttagc acgagtagat
     5821 cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa
     5881 ccttcacctg aaaaaagaac aattaatatg atgatatgac actatttgga tagcaggaag
     5941 gtttctgtca acaatcattg acacaaagac ggaaaaacgg gcacttcggc gtaaagatat
     6001 ccggtcagca cccgatattg cattattttt cgctgggcgt tttgcctttt tgcagagttt
     6061 ctttcacctg accgataagc tgacgacgga aatccccgag gcgtggcttg tcatcgtcaa
     6121 tccacggcag cgggcggcaa agctccatag ctttaatccc gagtcgtgcc gtcagaagtc
     6181 cagcaccaat cccctgagct gcccgggtag acaaacgcgc agcgagatct tgcgacatcc
     6241 agtccatccc cacttcgcgc accagttcgc tggctccggc gaaagcgata ttcagcaata
     6301 ccagcttaaa caggcgcaaa cggctgtaat atcccagttc aatgccatac agcgtggcga
     6361 tgcgattaat caaacgcaga ttgcgccagg cgataaacgc catatccacc agcgccagtg
     6421 ggctaacggc aatcattaac gttgattccg ccgctgagcg gctgatttcg cgccgcgcct
     6481 gggcatctaa aactggctgg accaggtgag catacaaact gaccacttca cgatcgttct
     6541 gcgtttcatg gattgaggca taccagcgct gcagcgccgg atgtgactga tcgatgcccg
     6601 cctgctgcgc cagtttttcg cagaatgcac ggcctttacc cgtgccgtga ctgtgcaaca
     6661 aatcgcgcgc ttcatcgcgt tcatgggcgc gctgtcgcaa gcgccataag cgccgccact
     6721 ctgttaccac agaacctacg ccagcgccga taatcaatgc cccagcggca catccaccca
     6781 gcgccaccca gtcctgggtt tgccaggcgt tcattgtcca ctgcacgccc tgcccgacaa
     6841 cgcttgcgcc aaacagagcc agcccgccca tcaccatttt gcgccacagg ctgcgtttcg
     6901 gacgtaacgc tgcgtccatc accgcttcaa cttgcccctc ttcttcctgt gcttcgtcga
     6961 gcgtggccgg ggcaaaattt tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt
     7021 ttggattctg atcgacctcc agtggaccgt cgaaatcaat acgtggtttt aacggttcgg
     7081 tcatcgcaat ttatctccta ttaaaaattc cagcgcagca tcaagacgaa tatgcggtag
     7141 cggtttgtcg acatccatca cctgcggacg aaacgcctca aactggaagc cttgcttatc
     7201 ccagaacgcc tgacctggca aacgtgcggg aacttcgcca ggataaacag tgagcggtgc
     7261 gccatcgctg agtcgattac cgcgcagcgc cgggattttc tcaccgttaa catcaatcat
     7321 tccgctggtg gtcgcctgaa cagacgccag ccccaggcag tccatactga tcccttcgaa
     7381 cgccgcattt tgccatgcat cctgaatcag ttgttgcagc aatgaaacca tattggcgtg
     7441 ctgatcgatg gtcacatggt ccgctttagt ggcagcaaac aatagcttat cgataaccgg
     7501 cgaaaacaaa cgccggaaca gggtacgctg cccgtagtgg aaactttgca tcagctgcgt
     7561 cagcgccaga cgcatatcat taaatgcctg tggcccactg ttgagaggtt gcaggcaatc
     7621 caccagcaca atctggcggt caaagcgcag aaaatgattc ttatagaacc ccttcaccac
     7681 tttctcgcag taataattaa accgctcgcg caacattccg gcattagtgt gcttatcggc
     7741 ctgcgccagt ttggactcgc cccaggcatc gacatccggc cacgggaaga attgcagcgc
     7801 gggtgctcct gccatatctc ccggcaagac aaagcgccca ggctgaataa agtgcaaccc
     7861 ctgctgtttg cagtgatgga gatactcggt ccaggcggcg gcaatgtctg ccagccggtt
     7921 ttcgtcggca ggcgctagcg ggtccagccc ttcgcacatc attcgccatt tcgccgacca
     7981 ttcgccgcgc tgaccattga gtaagcccgt catctggcgc gaccagctta aatagtcctg
     8041 cgccagcatc ggcaggtcga gcaaccattc gccggggtaa tccacaatct ccagatacag
     8101 cgtggaggta tccttaaagt ggcgcagcag cgaatcgttc gatttaaaac gtaacgccag
     8161 gcgaatttca ctgacaccgc gcgtaggcgt cggccaggcg ggaggatcgc catataactg
     8221 cgccaaccct tcatcatagg tgaagcgcgg aatgccaaag tcacgctggg gaatgcgttt
     8281 tacgcccagc aggcgctctt cacgcaccgc acttaaaagc ggcaaacgtg ctccggcatg
     8341 aatattgagc aactgattaa ccatcgcggt gataaacgct gttttgccgc tgcggctaag
     8401 tccggttaca gcgaggcgca gatgtctgtc gacaccccga ttcaccagcg cattgagttc
     8461 atttttaagt cgcttcattg ctgtcctgtg ctgctctgaa gaccagaaaa aaaatcgttc
     8521 gtaaggatat gaatgtgggg gccaggccat tgattatcaa ctacccgtgt tgtcatcgac
     8581 gggcgaactc cataagatgt gcagcattca cgttcggacg gagttcctga cctatgtccc
     8641 ctactattta tgatattgcc agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc
     8701 tgaataagca aaccaatatc tccccggaag cgcgcgaaaa agtgttacgg gccattgaag
     8761 aattacagta tcaaccaaac aagctggccc gcgcgctgac ctcttcgggt tttgatgcca
     8821 ttatggtgat ttctactcgt tcgaccaaaa ctacggcggg taatccattt ttctctgaag
     8881 ttttgcatgc catcaccgcc aaagcagaag aagaaggttt cgacgtgata ttacaaacgt
     8941 cgcacaatcc ggcagaagat ctgcaaaaat gcgaaagcaa aattaagcag aaaatgatta
     9001 aaggcattat tatgctaagt tcgcctgccg atgaatcctt ctttgcacaa ctcgacaaat
     9061 acgagatccc ggtggtggtg atcggtaaag ttgaaggtca atacagccat gtttattccg
     9121 tcgataccga taattatggc gacagtattg cgctgaccaa tgcgttaatt gaaagcgggc
     9181 ataagaatat agcctgcctg catgcgccgc ttgatgtaca agtttcagtg gatcgggtaa
     9241 atggttataa gcaaagtctg gctacgcata atattgcagt gcgtgatgaa tggattgttg
     9301 acggcggtta tacccatgag acagcattaa aagcagcacg ggaattatta agccagtcac
     9361 cgttgcctga ggcagtgttt gctactgaca gcctgaaatt aatgagtatt tatcgtgcgg
     9421 cggcagagaa aaacattgct attccgcagc agttagcggt ggtgggttat agtaatgaaa
     9481 cgctgtcgtt tatattaacg cctgcaccag gcggcatcga tgttccgacg caggagttag
     9541 gtcaacaaag ctgcgagtta ttattccagt taattgccgg aaaaccgtca ccacaaaata
     9601 ttaccgttgc tacgcatatg tcgttgaaat aaatgtatgc cggatttggc gctcgccccg
     9661 catccggcaa tttagcacat tatcagaacg agtaattcac cccaacaccc gcataatgga
     9721 atttatcgct gtcgccttcg tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg
     9781 aaagaccatt ctggaaatca taaccgtaaa ataaacccac acggttaaaa tcatggcctt
     9841 cacgttcaat atcatcctgc cagtcccagt tactccagcg atccagccca atgcgcgtat
     9901 acggcgtcac cgagtggttg ccgagcgtca gcggcaaata ggcgcgaatt tcttgcgtgg
     9961 aaaactcacc gttattgcgg ctatcatcca tattgaaacc acgctcgaga taatagttca
    10021 ctcgcaaggc aaccgtttcg ttgaaggtat attgcagacc cgtttccgtt tcgacacggg
    10081 tatcagcata accggtcgta ttcagatcgt tggcaaattt atacatcgac agccaaccgt
    10141 tgaaacgtaa atcgtcagtc agtttcacat cccagtctgg cgcgattttc cagcgctgca
    10201 tatttgccgt gtctttaccc ggttcatcaa cgtagtgata accatagtta cggaaaccac
    10261 cagtcagacc gagactgaaa tcatcgcttt cgaggaactg ataatgtacc tccagctccg
    10321 ggcgatcaaa ccacgttcca cgtttacccg cgctataatc taccggccct tcctgataat
    10381 aggccagaga aattctccac ggcccgttgg cggcattaaa atagacagaa ggctctgcca
    10441 gcccatccat atcttcgaca taaccctcga cgttttctat ttcgtacatc tcgccgatat
    10501 taaagtgcca gttgttcttt tcctcggcct gtgcgcaggc cattcccgca cacatcacca
    10561 gtgcggtaca gggtaataac tttttcatta ttgttccttg tattatcctg ggagaatgcc
    10621 ttccccctgt atttagcaaa ttgctatttc cgtttctgca tcaaagaaat gacatttagt
    10681 catatcaaaa tgaatagtga tattttctcc tgcatgataa tcatttaacg caccagcacg
    10741 gaccactaac tcgtgccccc caaccgtggt gtagagcata aattccgcac cggttaattc
    10801 cgccacgcta attttggcgg aaatgttatt ttcttcttgc gcatccggat gaatatcctc
    10861 cgggcgtatt cccatcacaa tgggtttatg taaactttcc tgcgttttca gaaccgctaa
    10921 tttctcttcg ggaatggtta atttaagtgt ttccgtaacg aatttatcgc catcgatcgt
    10981 gccgcgaata aaattcattg ctggtgaccc aataaatccg gcaacaaaca tattcgccgg
    11041 ttggttatag accgttttcg gcgcaccgac ctgctgaaca atcccatcct tcataatgac
    11101 aatccgcgtc gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt
    11161 gttcagtttc tgatgcagct tgctgatctc tgcgcgcatt tgcacacgca gcttggcatc
    11221 caggttagag agcggctcat ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc
    11281 aagcgccact cgctgacgtt gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc
    11341 acgcaggccg aggatttgcg ccgcccagtt aacccgctca tcaatcatct ctttggcgat
    11401 tttttgtatc ttcaggccaa acgccatgtt gtcgtagacc gtcatatgcg gatacagcgc
    11461 gtagttctgg aacaccatcg cgatattgcg tgctttggct ggaacgtcat tcattcgttt
    11521 gccgtcgatc aacagctcac cgccgctgat ctcctcaagc ccggcaatca tacgcagggt
    11581 ggtcgattta ccgcagcccg acggaccgac aaacacgatg aactctttat cggcaatctc
    11641 caggctgaag tccttcacca catgcacctg gttatcgtag atcttttgaa tatgttgtaa
    11701 cgaaagctgt gccatgttga ttcctttgct atacgttttg ccagaaggcc gataaccgcg
    11761 gccaggtcag ggagtccgtt gagggcaaca ataattgcgc cccggttaag cccgcgccga
    11821 tccccaccga gcgcataccg ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa
    11881 tgccgataca tgcctgtggc ggcacgccca gccctgcaca ggcggcgaga aagatttccg
    11941 ggtccagttt cgaatttttg agttgggaag cgtccgcgca gaaggtgaaa aactcgcgca
    12001 gctccagcgc tgctaaaatc gtcggcgcat tcagggagac agaagccagc ccaactggaa
    12061 tctgctgtgc ccggagatct accagcaaat tgtgaatgcc aggcagcaca gcattaaccg
    12121 tcagttcgcg cagtgagtgg acgtaaagca gatttttgcg atacgccagt tgcgcccgtt
    12181 cctgtgggtt aaaatcgccc tctttacccc cgtgctgcag aattcgccgc agggactcat
    12241 cgcggctgat cccttttagg aattcgttaa actgcgcatc aatgctgatg ccaatttcag
    12301 cggcaatctg ctgccacgcc tggaaatgca gatgcgcggt atcggtgatt actccatcca
    12361 gatcgaaaat taccccttgc agtttcattc accctcctga tgcgtggtag cggtcccatt
    12421 gaagggcaaa ataaaatccc ccaaacagaa aacagattct tcagcgacgt aaataagctg
    12481 accgttcaag cgcagtgaaa caggcgcaga agttcgaatc gcaatacgct gcgcgtcgag
    12541 agtgacctgt aattcgcagc cctgccagaa cagagggaaa gacaactgtt gccactgctc
    12601 aggtaacgcc ggattgagat gtaattcacc gtcacgcacg ctcaccccgg caaaaccctg
    12661 aatcgccccc agccagatag cgccagttgc ggcagcgtgg ataccgtcat cacagctatg
    12721 cggatcagca ccaagatcga tttcagtccc ctcgcgccag aactgataac tttgggtcag
    12781 caggccacag cgtgcggcaa caatgccgtg gattgcttta cttaatgacg agtcgtgaat
    12841 agtgcgcggt tcataaaatt gcagattggc aagacacgat gccgctgaga actgctccgg
    12901 cagcatgtaa ttgagcatca ccacatcagc ttgtttgagg atctgcatct cgttcacttc
    12961 tgcgcgtgaa taatccagca gaatggtttg cttccccgcc gccgctttgt atttcgccag
    13021 attaatcacc ggcttagcca taaacgaatc atcctgcggc aaaacgccgt cgggctgaat
    13081 ttctggcagc cgcagttctt tgaggtacat ttctgcacga tgaataaacg cgtcgtcgct
    13141 acagccaaac tgacgagcga tattcagcgc ctgctggacg ttgtaatacg ccatatagct
    13201 ggtgaaggcg ttattattga catgttcggt atattcgtct ggcccaataa catcatgaat
    13261 ttccagacgc tcgttaaccc tcaccgcgcg gctaatccag aactttgccg tctccagaag
    13321 tagcgccatg ccttcatgag caatgaaact ttcatccccc gtggtccgcc agtattgaat
    13381 caccgcccag gcgatatcgg cgaccagatg gtgttccgcc tgcgcagagg ccactttttg
    13441 ccgcagcccg gtacgaatgt taatggcggc aaattccggc gtctcttctt cgccgctacg
    13501 cgcgctttcc cacggaaaca gagcgccctg ccagccgttg cgtcgcgctt tctcctgcgc
    13561 gccaggcaag ttgtgccagc gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa
    13621 cagatggaac ggtagcaaaa atacttctgt atcccagaaa acgtggcctt tgtagccttc
    13681 gccggttaaa cctttcgccg caatgctgct gcgctcatcg tgggcaggcg tcatgatgcg
    13741 caggtgataa agcgcataat ctaatgcttg ctgatcgtgc gcatcgcggc cgtttaccgt
    13801 gatacgacgt ttctgccacc attgacgcca gttttctgtt gatgctgcaa gaagttggtc
    13861 gtaactctgc tgcgcgcaca tttcaagctg acgaagcgac gcactgcccc attcgtctaa
    13921 agcggcttgt ctgtcgtccc gccagtcgat ccacaccagt ttttgcagcg tcagcgtctc
    13981 acctgcgtga agctgcgcgc aggtatgttg caataaacgg cgctctttgg cggtatagca
    14041 ttgctgcaca tcaccgctca ccttacagca acagctgatg gccacatcac tacgcccatc
    14101 ctgggtggtg tagatcccct gcatcagatg ctgaccaaac acccgcacct ggatttcgtc
    14161 aatatgctgg cgaccgtggt tggtttgcgt ggcgtcaatg cctgttgaaa tcagcactga
    14221 cgcgtcggcg tccagtggag taatagtgat ttccagcgca atgagcggca gttggtctgc
    14281 cgaaacaaag cgacggctgg cgatggtgta accagtgccg ttgctggtac gccagacaac
    14341 gttgcgacgt aattcgccac tggcaaagtt aagctcacgc tgccatgctt cgcgggataa
    14401 cgagaaaatc tcaccattta tggcaatctc catccccacg acatcaggca ggttcaccag
    14461 ttcgttgatt tctcctttac ccgctcgatg atacagcccc gccagataca tcccacgggt
    14521 ctggcgcgtg taatcttctt catggctggc acgaagccca agatagccgt tcccctgcgc
    14581 catcagcgaa gcatacttgt tcagggtatg ctggctgaaa tggggttctg ataacgttac
    14641 tggcctggtc ataattccac acgcgtccct gtttccgcgg attgatacaa cgccgccacc
    14701 agttgctgga tgatgtaccc ctgctcggcg tcggctatca tcacgggctt gccctgtacg
    14761 tgattgataa aggcttccat gctgcgcaaa tggcggttgt cgtctgccat ttcccgttgc
    14821 atcagcgtca ttaattcacc gttgttatcg gtgtagatat gtgccggaaa cagcgtcgca
    14881 ccggctttat caccacaaaa gctgacattc ataatcgact gttcgcggat gttgagtgca
    14941 aatgacgttt ccagccacag aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa
    15001 tcttcaacgc tgtaagttgc cgggtcccac tcgccaaatt gaccacagct cttttgcgtg
    15061 ccgatctttt gaaagctatg cgcattcacg cttttcaccg ccggaaagcc cagcacatac
    15121 atcgcagcat ccagcatatg aatgccgatg tcgatcagcg ggccaccacc ctgcagttct
    15181 ttattggtaa agacacccca gccgggaacg ccgctgcgac gcagggcgcg ggcggtggta
    15241 acgtaaatct ctcccaaaac gccgttggtc acctgttcac gcagctgttg cgtatcgaac
    15301 gcaaaacgat ggtgaaagtc gtaggccagc accttgccct gtttgcgcgc ggtatcgcac
    15361 atttcccgcg cctgttctgc cgtcatggcg ggcggttttt cgcacatcac atggcagccc
    15421 gcttcaagcg ccatcagggt atgttcgtaa tgaaaacggt tcggtgagca gacgctaacc
    15481 acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacaagga tgcattaccg
    15541 tatttttctg ccagcgcctg cgcctgggaa aggcggctgt cacagacggc caccagttcc
    15601 agatcgttgc gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcctgcgccg
    15661 attatcgcga cacgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag
    15721 gtgcgcaacc aggccaacga atcacggtac gcctgggcag gagcttctgc ccggatacgc
    15781 ccttcataca ccacataacc ctgatagtta tcggcgcgca gctgttcaaa cagcgcgtgg
    15841 aaatcgaggg tgccgctgcc cggctggtaa cgatggttat cggcaatatg cacatgaccg
    15901 agcaagtcgc ggtggtcatg cagcgcctgc gccaggttat cttcttcgat gttcatgtga
    15961 tagaaatcgc cgataatctg cacatgttta agatcgtttt cgacgatgta acggcgggca
    16021 tcggcgagag tattgatcat atgatcctga taacggttta acggttcgag atacaccacg
    16081 gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cattttgcgg
    16141 tcgccgtcca ggctgcgcgg cgaggtcatc ggcggtaaac ggaaggtaaa catgccccat
    16201 gcagcaggaa cgacgatacc tttaccgccc acttctgcca gcgcttcgag aatgcgttcg
    16261 atctgcttta agccattaag acgacgctct tcgataaaat caccaatcca gccgtcatag
    16321 ccgccgcagg cagtggtcac cggtaaaccg gtttctttga tcgccgcttt gacttcttcg
    16381 atgttgttga ccagcagttt gccgtcaatc tcaaaaccat cgaagcccat ctctttgatg
    16441 taacgaaatt tctccagaat atttcccgga aagaatgcct gattctgtgt gcctattttc
    16501 attttttcat tcctttgaat taaaacgtaa cgcccatttt gatgctctgt tccggatgct
    16561 gatcgacata ctgcatgtag ctttccgggc tgttggcaaa ggtcactacc gggtcgatta
    16621 aatcttcgca attgagataa ccgttcatca gcagttccca gcaggtttct tcaatacgct
    16681 tacggctcca gcgcggataa tccgggttcg gttcgctgca cgcgcgggag aaaacaattt
    16741 tggcgttatt gaaatgcgct tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa
    16801 acgcgacata ggagatggtg ccgccgtagg ccagaccgcg gagcgccgac tgcagcgcgt
    16861 cggcgtagcc gctggtttcg ataatcacat cggcaccctg cttgccggtc agcgttttga
    16921 tctctttacc tacgtcagcg ccaatggggt taagacagaa atccgcaccg tggcgacggg
    16981 caatatcaca gcgatgagcg ataggatcga caccaatcac caccgacgcg ccagcgcgtt
    17041 ttgccagttg aatggcaatt tgaccaatcg cgccaagccc taccaccacc acaaaatcgc
    17101 ccacgcgcac gttggcatcg cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaga
    17161 cggcgttttt ccaggagctg ccttgcggca ttttgcgcag cttgtagtta ttcactgcgt
    17221 taatgatgac cgtctcggag agcgggccgt agccgcatac gctgtcgccc accgcgtagt
    17281 cgttaacgtc gctgccgcac tcgataatgt cgccaaccac catgttgcca agctggaact
    17341 tgccaaactc aatgccgcgc ggcgcatttg ccggacgcgg gctgaacatc tgccattcac
    17401 cgttaaagtc ttcatcgata aacggactgg cagcgcggaa atcgaccact tccgtcccgt
    17461 gcttcggagc gccgaaacgc acgcggatct tcacttcatt ggctaaaatc gcccgctctt
    17521 catactcaac cagcgcagct acacgcggtg ctgtggctac taactttttc atgtaaaact
    17581 ccttgtaaag aaattggtcg ctttagccct tcacgccacc agcggtcaaa ccactcttaa
    17641 taaaacgttc cgaaagcgca tacataatca ctaccggtag tgcggtcacc agcgaggccg
    17701 ccatcatccg tccccagatg taatcaggcg tactgaacag cgcgttcagg cccacaggta
    17761 aggtgaaatt gctggcgctg gaaaggaaaa tcgacgcaaa cagatagtcg ttccacgcca
    17821 ccataaagca gtagacgaat acggatatca gcccggacat tgccaggggc acggtgatac
    17881 ggaagataat ttgcagacga ttgaggccat ccatcattgc cgcttcttcg atttcatcgg
    17941 ggatggtgtc gaagtagctt ttcagcataa acacggcggt aggcagagtt tgcgtcacca
    18001 tggtgataat cagcgccatc tcggtgtcgt aaatgccaag tgcggtgatg attttgaaca
    18061 gcggcaccac cagcaaaatg ccggagaata tgtacaccgt gtaaaagctg gcgttgatgg
    18121 tcattcgccc tttaaagcgc aggcgggaaa gcgcgtaagc cccaagaatg ccgagaaata
    18181 ccgccaccac ggatgaaacc accgacacca ccatgctgtt acggaagtag tcgacaaacg
    18241 ggaaaatcac cggattaaaa atgtcgacgt aatgctccag cgtccactgc tgtggcagca
    18301 acgtaggatg cagcgagatc gcctcttttg cgctcttgaa cgaggtcatc agcatcacaa
    18361 aaaatggaaa cagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa ccgattcgac
    18421 tgagtgtgcg tttatttgtt gccattgagg ttcacccgtt tcctggtcag cagaatgaca
    18481 gcgaaaataa tgatgaaaag caccaccgag atcgccgccg ctttccctaa gtcattaaag
    18541 gcaaaggcgg ttttgtagag atagacaccg agaatatcga ccttggtcgt cagcagataa
    18601 acatccgcga acatgtagaa catccagata gtgcgcaggg tcaccaccgt cgccagaacc
    18661 ggcataatcg cgggcagcgt gacgatgcga aaccgttgcc aggcattcgc gccatccatc
    18721 tctgccgctt cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag
    18781 gcatacggga agtagcgcca gatggcgaac agcaccacca gcacaaaact actgcccggg
    18841 ttatcgaacc acagcggcgc ctgctcatag agatgcagaa gatcgacgcc gaggtagttc
    18901 acaatgccgt agccgttgtt gaacatgtat ttccaggcga acaccagcga aatagacggc
    18961 gttacgtagg aaaggatcac cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg
    19021 aaaaacatcg ccacagccag cccgagcacg gtgctgcccg ccaccacaag cgcggtatac
    19081 cagaccgtca tccacagcga gtgccagaaa ccaggatcgg agagaatgcg cacatagtta
    19141 ctcaccccaa caaacgttga ctcgatgttg ggattaagcg gcagacgtaa aaaactgatt
    19201 tcgatattcg acaccatcgg ccacgccacc agaccgccca gcagtaataa gctgggcgcg
    19261 agaagcaaca gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat
    19321 tcctgtcctg ggtttagcgc tgttcgacca actcatccag ctttttctgg ctcgcttgca
    19381 gcgtgccagg gaggtcggct ttacccacgg tgacgttatg caccattgaa ctcaccacac
    19441 cagaccccgt cacatcaccc atgcgggtaa agtttttatc ccctactgcg ccaaagacct
    19501 gaatatttgg caattcgctg attagctgat tcggcaattc tcccagcgcc ttaataacgt
    19561 cgttgtcttt ccaggtggcg gtagtgacaa ccgctttgtt caccggcagc gccgcgcctg
    19621 gtgacatcat cacccagtcg gcaatgttgt ccgcctgctc cataaaggtg acaaattttt
    19681 cagccgcttc ggtctcttca gtcttttgcc cggcggtaat ggtcagcgag gtcaacatgc
    19741 cgtagaccgc agaatttttc tcggttggca ccacgaaacc aacgtttttc ggatcgcctt
    19801 ctttaatcac agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca
    19861 taaaagcgtc tttcacttcc atgatgtcgt tagaacctgg catggtgttg gcagcaaggt
    19921 tgcggtaata ggtcagcgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
    19981 cgttaaagac gttagcctga ttggataacg caaactggga gaaagactgt tccgtcaaca
    20041 cgctttctgc tgtaggcagc gcaataccgt attttttatt cgccgggtca ttcagtttct
    20101 gtgcaacgtc cagcaattgc tgccagtttt ttggctcctc cagcccagct tttgccagca
    20161 catctttgcg ataccagatc ccgccaatcc atgcgctgac aggaacaccg gtccaggcgc
    20221 tgccatcttc ggtgcgcaca atccgcagta cgccatcgta gaacgcgcct tcaccgacgt
    20281 tgctgatgac cgtggcaacc gctttacgat cgataagctg ctctttgtcc atcactttgg
    20341 cgtagtcatg gctggtttcg atcacttccg gcagcgaacc gctacgtgaa agggtaatga
    20401 ctttggtgtt ataggcgtct tcttccacgg gcacttgttt tacggtgata cctgggtttt
    20461 ctttttcaaa acgggcaatc aatttgctga tgacggcctg gcgctcctgc tccaccgaag
    20521 aatgcataaa ttcgatggat acattatttt tattttcttc tttacagcct gaaatcaggg
    20581 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaaa tgttcctttt
    20641 tatttaatcc acataacctg ccagggatgc agtgttattt cattacccaa tatatcaact
    20701 tcactaatca gatcccgacc gtgcaggtca gtaatattta tatgctgaat attttcgctg
    20761 acattaaaca atcccgtcag gcaatcgcca tccgcattgc ttctttgaat tcgcattaca
    20821 aatgaattaa ttgcatcgat ggtgaaatta ttatccggat gaaactcttt atgactgcga
    20881 cgaagggtaa ttaaatgcga caacttataa tataccgaat accttaatgt cgctttattg
    20941 tccagttctg tcgttatctc tttactagag tattttttac ggttaatcgc gcgattatat
    21001 ccaagttttt cgacaccggc gtaatcatta cgcgacccca gaatgctttg aatatatatc
    21061 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcacaccgt
    21121 tcttcgtcgc tacactcacg gcggctcaac gcatccatat aggtgacatt catttcatag
    21181 gggctacgcg taccgtcggg attatttttc cagtttacta atgccccttc ctgctgtaac
    21241 acctcgacca gcgctaatat ttcgttttcg ggtaacaacc cccgcagtgg gttaagcccg
    21301 atgccatcgt gagaggcaag gaagttaaac caggtggtgt tgctggaagg tagtgtcagg
    21361 ttttgcgccc acgcacaaag tgcctcaacg ttctgttttt gcaccgcatg cagcaccagc
    21421 ggcggcagcg agaactggta caccatatgc gcttcgtcat cgccttcgcc aaagtaagca
    21481 atgttgtctt tatgcggaac attggtctcg gtaatgatca ctgtgcctgg cgcgacgtta
    21541 tcaataatcg aacgtaacag tttgataatc agatgtgttt tttccagatg gatgcagttt
    21601 gttcccggct ctttccacat aaagccaacg gcatccagac ggacatactc tgcgcccttt
    21661 tccaggtagc acagcaatac atccaccatc gccagcaaca cttcagggct acggtaattg
    21721 aggtcaattt gatcgtcgct aaaggtggtc cataaatgac gcgttgaatg atcgcgcatc
    21781 tggaatggcg ttaataacgg taacgcacgc gggcgagtga cggcgctgag atcggttttc
    21841 ggatcaacgg taataaaaaa atcttcaaaa cctggctgct gttgtaaata gtttttaaac
    21901 cattcacttt ttgccgacat atggttgcag acaaaatcaa acattaaatg gctgcattca
    21961 ccgagttgct gaatatcctg ccactccccc gcttcactgg cgacctgatg ataatcaatt
    22021 accgaaaatc catcatcaga tgaccatgga taaaaaggta ataaatgaac atgtgaaaaa
    22081 attctttgca gccattgagg gtaaaactga ttaaatgtgg gtagtggctt taaatcatta
    22141 ctgtgaaatt gatcggcata ggtaattaac acgacatcac tttcatccca gtgtttttta
    22201 cgtcgctgtg taattaatcg tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga
    22261 gtcgcggtaa atgttccacc gtagatttcg tccaggtaat ccgtaatttt ctgttgcatg
    22321 ttgcctttcc gtggtagcgg tcccatgaag cgagagacta ctccgcataa aataaactgt
    22381 caattgagct caaggggttt agtcacgcag gaaatctttt tttacctggg aaaaagcgta
    22441 tttgtgagct gacgcaaata atctatgggg atgtaaataa ggtgtgacag aaaaaaacgg
    22501 cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct
    22561 ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgcc
    22621 tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg
    22681 gcggtagcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc
    22741 tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc
    22801 agtgaaaaca ccagtgtcag agcaagtaag ccttttttaa acatgtaagc gtccttctat
    22861 ggatgaaaat tgctatccat tatacgcctg agatttaacc atttttgcga aactcccctc
    22921 accttttata acgctgcgcc agtttattgg cagcccgact taacagcggt tccagcgcca
    22981 cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac
    23041 ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaacccag
    23101 gcttcacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg
    23161 gaaacggcta cgcagcgtga aagtgtcgga agtgacataa cgttccatct cgcgtaaacg
    23221 cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag
    23281 ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta
    23341 agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac
    23401 cagttttact ggtacatcaa aatagttggc aatcccggcg cagacgccac ggaccatgcc
    23461 ctgctgcgga atacgccata attttttatt gagattaatg cccgccatta gcgatccctc
    23521 cagttcggat gttctgcatc aagaatggat tccagcgcct gaatacgctc gcgcatccgt
    23581 tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgtgacaa ttcactgcga
    23641 ccggaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg
    23701 gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc
    23761 gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt
    23821 gtgccagttg ctcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg
    23881 atttttgttt accgaagctg tggctttctg cttccgcttc catctggtcg atacggcgtt
    23941 cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca
    24001 catcgcgcga cgagtttgcc gcttgatggc gtaacatcaa tgcctgctgg cgagcgcgtg
    24061 tttcgctcaa tttgttttcc agctcgccga tctctttctt catacgcact agcgtatcgt
    24121 ccaccagcgt cacttcatgt tccaggctct taatcagatc ggtcagtttc tgtttttcaa
    24181 ttaacgccgc acgcgccaga tcctctctct ctttcagcag tgccagttcc gctttttcct
    24241 gccattcaat ttcacgagcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg
    24301 ccagcgcacg cgccgaagta gaacgtactt caaccaatgt atcttccatc tcctggatca
    24361 tcagacgcac cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat
    24421 tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt
    24481 caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt
    24541 tataaagatc tgattgaaga atcaacagca acatgccagg atgaattagc gaattacact
    24601 aacaagtggc gaatttcatc atggcagaat acaaagataa tttactcggt gaggcgaaca
    24661 gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca
    24721 tcatcggcga gcgcggcacc ggtaaagagc tgattgccag ccgtctgcat tatctctcct
    24781 cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa aatttgctgg
    24841 attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatccag
    24901 ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga
    24961 tgatggtgca ggaaaaatta ttgcgtgtga ttgagtacgg tgaactggag cgcgtcggcg
    25021 gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg
    25081 caatggtcaa tgaagggact tttcgcgctg acctgctcga ccgactagcg ttcgatgttg
    25141 tgcaactgcc gccactacgc gaacgtgaaa gcgacattat gttgatggca gaacactttg
    25201 ccatccagat gtgtcgggaa atcaagctgc ctctgttccc gggttttacc gagcacgcca
    25261 gagaaacatt gctgaattat cgctggccgg gaaatatacg tgaattgaaa aacgtggtgg
    25321 aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc
    25381 cctttaaacg ccgtccatct gaagaagcca tcgccgtttc agaaaacacc tcgcttccga
    25441 cgctgccgct ggatttacgc gagtttcaga tgcagcagga aaaagaactg cttcaggtca
    25501 gcttggaaca agggaagtat aaccagaagc gcgccgctga attgctgggg ttaacctatc
    25561 atcagttccg cgcgttgctg aaaaagcacc agctttagca tttcgacaat gcaacttgcc
    25621 gggtgtgccg tgaacgcctt atcaggccta caaaatcaat acgtcgtttg caggcctgat
    25681 aagcgctgcg catcaggcat tattgcgtta accctgcttc tgcgtattaa tctctcagcg
    25741 catcctgcaa aattttcatt gccgcatcga attgcgcaac cgggatggta tgggcttcgt
    25801 taggcggcgc atatttgtgg tatttgatcc gccgctatcg caaagtgcca ttgtatgatg
    25861 gcgacagaac gccggtgagc gaaacgtaat agtcgaatgc gcggcgcatt atcctgcctg
    25921 agggtaatgc accgcgccat taactataaa aatatgtatg acccggatca cgagtgctat
    25981 catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg
    26041 ctaaaatatt atgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac
    26101 gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat
    26161 gaagagatca gtgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
    26221 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc
    26281 agactatcgg tcagtatcgt tgcccggata aagcgttcgc gccgcatccg gctaaaaatg
    26341 acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
    26401 agttgttctg tgagttatag ttataaagaa atcacgggta cgaatgttga actgatggcg
    26461 acgccacctg ttcaatgacc aaccacacgg ccctcattat tacctctgaa cacaaacggt
    26521 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt
    26581 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
    26641 gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc
    26701 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
    26761 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgtctgatg
    26821 ccggaacttg ccgaaagctg ggaagtactc gacaacggcg caacctatcg cttccacctg
    26881 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
    26941 gacgatgtgg tttttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtt
    27001 aatggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc
    27061 cgcaaactgg ataatcatac cgttgagttc cgtctggctc agccggatgc ttcttttttg
    27121 tggcacctcg cgacccatta tgcttcggtc atgtcggcag aatatgcagg gaagttagag
    27181 aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg
    27241 tcggaatacc gcgccgggca atatattcgc ttacaacgtc atgatgattt ctggcgtggt
    27301 aagccgttga tgccacaagt ggtggtggat ttaggctctg gcggtacagg acgattgtcg
    27361 aaactcctga ccggggagtg cgacgttctg gcctggcctg ctgccagtca gctatccatt
    27421 ttgcgtgacg acccacgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg
    27481 gcattcaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg
    27541 gcaattaata accagcgcct gatgcaatcc atctattatg gtacggctga aacagcggcc
    27601 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat
    27661 ccggcaaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg
    27721 tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaaccgc cgaactgatt
    27781 caggcggata tggcacaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgtttt
    27841 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac
    27901 agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcgcag
    27961 accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
    28021 tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag cgcaaagtat tctggcgcag
    28081 gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
    28141 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
    28201 caggatgagg tgaaaaagcc atgattatct tcaccttacg ccgtattttg ttattgattg
    28261 tgaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcacc ccacatgcgc
    28321 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact
    28381 gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct
    28441 tcccggcaac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
    28501 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
    28561 tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt
    28621 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga
    28681 aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcaccg tggcgggatg
    28741 aaatgatcat tagcgcaatc cgccatatgg tattgcccgt gatcaccctg tcggttgcgc
    28801 caacaactga agtgatccga ctgatgcgta tcagcactat cgaagtgtat gaccagaact
    28861 atgttaaagc agcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
    28921 tgcataacgc gttacctccg gttattcctc gcctgggctt acagttttcc accatgctga
    28981 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
    29041 acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
    29101 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
    29161 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
    29221 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
    29281 gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag
    29341 caatttctcg gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc
    29401 ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg
    29461 ccaaccgtag gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg
    29521 ggcaccttcg ccggggcgac ccacggccta cgctcagcgg tgcttaacca tattctggat
    29581 accttactgg cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt tgccggaccg
    29641 agtttgtctc acgccatgtt tgctgtctgg ctggcgctgc tgccacgtat ggtacgttcg
    29701 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
    29761 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc ccaacatcac cgccgggctg
    29821 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
    29881 ctcgatctcg gcgcacaact gccctcgcct gaatggggag cgatgctcgg tgatgcgctg
    29941 gaactgattt atgtcgcccc gtggactgtc atgctgcctg gtgcggcgat tatgattagc
    30001 gtgctgttgg ttaacctcct tggcgacggt gtccgtcgtg caattattgc gggggtggaa
    30061 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
    30121 ttaaagccgt cgaccgcgta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
    30181 gcgaatccgg ttccggtaaa agtttgattg cgaaagcgat ttgtggggtg aataaagata
    30241 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
    30301 cacgcgaacg gcgcaagctg gttggtcaca acgtgtcgat gattttccag gaaccgcagt
    30361 cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
    30421 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
    30481 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
    30541 ccgaaggcga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
    30601 tgattgctga cgaaccgaca aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
    30661 tcctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
    30721 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acggtggaaa
    30781 ccgcgccgag taaggagttg gtgacgatgc cacaccatcc ttatacccag gcgctgatcc
    30841 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg
    30901 gcgctatccc gctactggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt
    30961 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
    31021 cctgtcattt cccactgaac atggagaaag agtgagatga tcgaaacgct acttgaagtg
    31081 cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa
    31141 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tatcggcgag
    31201 aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga acctaccagc
    31261 ggcgagttat tgattgacga tcatccgctg cattttggcg attattcctt ccgcagtcag
    31321 cgtattcgca tgatttttca ggatccttcc acctcgttga atccccgtca acgtatctcg
    31381 caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa
    31441 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
    31501 catatgttgg cacccgggca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc
    31561 ccaaaagtca ttattgccga tgaagccctc gcctcactgg atatgtcgat gcgttcgcag
    31621 ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc
    31681 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
    31741 gttgtcgagc gaggcagcac cgcagatgtg ctggcatcgc cgctgcatga actcaccaaa
    31801 cggctgatcg ccggtcattt tggcgaggca ttgacggcgg atgcgtggcg taaggaccgt
    31861 tagtgttttg cacctcatcc tgacaggccc ccctcaggat gaggtgttca ttacttttgg
    31921 ttgtcgattc tggtgagagc aaccagcaat gccagcgtat aacacaccgt aattgccaga
    31981 ctcattttta acagtgtttc tccgccaatt cccgacaatg gcgcggctat accgccgaag
    32041 acaaacatgc tcatccccat caacgcagac gccgttccag attcctgcgt ccctactgca
    32101 ctcatagcct ctgccccgcc aacagtgccg atgccgctac aaaatgcgat ggtgaaaaat
    32161 aatgccacca gtgccggaat gggcaattgt atccatgcgc ataagaccgt gagcaatgca
    32221 cacaaaattg ccgctatcag gccacctcgc aacaatgtca tcgcgttaat ccgtcgcgcc
    32281 aggcgcgaga agatccatga agcaatgata agtccggtgc cgttaaggcc aaacaccagg
    32341 ctaaattgca ttggactaaa gccaaattcc ttctgcaaca cgaacgacga agagccgata
    32401 tatgcaaaca aaccggccag cataaaactt tgaatcaggc aaaagcgcat aaaacggcgg
    32461 ttctgcacca cgcttcgtcc ggttaatagc aatgatgagc cccttttatt ttctggcaag
    32521 gtctcatgaa taaataacag acagcccagc aacagtacgg tgctaatttc agccattacc
    32581 cagaataaag tgcgccagtc aaaagtgcta acaatgtacc cgcccagcac tggcgacaac
    32641 accggtgcca ggccattcac cgtcattaac agcgcaaaaa actgggtcaa cgttactccc
    32701 tgatatttgt cacgagcaat agaacgagag agcaccgaac cacccgcccc cgcgatccct
    32761 tgaataaaac gccagaccac cagccagtaa atattgttcg ttgtcgcgca taaaatggaa
    32821 gaaacaataa acaatagcaa cgacaagatt aacggacgtt tacgccctat tttgtcgctc
    32881 agagggccaa ataacagttg tccgacgcca agaccaatca gtgatgcggt cagggttaat
    32941 tgcgttatgg tcgtggttgc cgccagttgc tcagacatct ccggcaaagc cggcaaataa
    33001 agatcggtac acatcgggcc gataccggct aaaagaccaa gaatcaatgc ccatgaaaga
    33061 gagactctag ccatattatt ccttacttga gtgatgacat accgccgccc agcgactgcc
    33121 agagcgtgat tcggtttgta aaatcagtca gttgcagtgc cagcagctcc tgctgtgcgc
    33181 tccacaagct gcgctgcgcc gtcaacacgg tcaggtaatc accaacaccc gcctgatagc
    33241 gacgcaagcc aacatcgacc gtttgttgtt cagctttaac atactggcgc tgtgcatcca
    33301 gttgctcttc aagcgtggtc cgccgcgcca gtgcgttagc gacatcttta aatgcgctct
    33361 gaacggtttt ttcataggtg gcaatgagtc cacgcttttg tgcttctgcg taacgaagct
    33421 gcgccagatt acttccgcca gtaaacagcg gtaatgtgac cgatggcgca aatgaccaga
    33481 tctgcattcc gtggctgaac aacgacgaca acgcgtcact gccaacaccg gcactggcag
    33541 tcagcgaaat cgtcgggaaa aagttagccc gcgccgcgcc aatatctgca ttggcgctct
    33601 tcaaattatg ttccgcttcc tggatatccg ggcgacgcag cagaacatca gatgatactc
    33661 ccgcaggaac cagacttatc atttgttccg gcaggctttc cagtgttccc ggtagcagat
    33721 tttccgcaag cgttgtacct gccagtaagt tcagcgcatt tttatcctgc atcacttgtg
    33781 tctgatagct ggcaacgctg gcgcgcgctt gctgataaac actcatcgcc tcactgacat
    33841 ctgtcgctgc cgccgtgcca acctgttgct ggcgctggat aattttcaat gagttttctg
    33901 cactggccat cgtttctttt gccagggcca ggttgctgtt atcagccgcc agtgtcagcc
    33961 aggcggtgct gatttcagca atcagcgtta aacgagtgtt ctgggctgta aattcactgg
    34021 caagccaggt ttcccgcgcc gcccgagata aactctgatt acgaccaaac agatctaacg
    34081 tataactgga caccgtgccg tcagcctcgg cagttgttcc cgtaccattc gccagcgaac
    34141 ggctgcgggt cgaacttaag gccgcattca ccgtcggaaa taacgacgca ttagtttgcc
    34201 catagagcgc ccgtgctgag tcaatatcag caatcgcttt ttgcacatcg cggttactgt
    34261 tcagtgcaat ggttactacc tgctgcaatc ggggatcgtg gatcacctgc tgccagtcat
    34321 ggctgattgc cttcccctgg ccctgtgctc ccggcaaggt cgccgggatt ggggattcag
    34381 gcgtactgta gtgaggatcg agcgagacac atcccgcact caacaacacc agaaaaataa
    34441 gagaacgacg caacataact tactcctgac ggcgcggttt accggcaaac aaacgtttca
    34501 ccagtacaaa aaacagagga acgaagaaaa tggccagtaa tgtagcggtc agcgtgccgc
    34561 caataatgcc cgtaccaatg gcgatgcggc tgttcgcccc tgcgccggtt gctatcgcca
    34621 gcggcattac ccccgcaata aacgccagcg aggtcatgat gattgggcgt aaacgagtct
    34681 gcgccgcgcg taatgccgca cgactcaagg aatagccctc tgcaaccgcc gcttcagcaa
    34741 attccacaat cagaatggcg tttttcgacg acaagccgat agtagttaat agcgctacct
    34801 ggaagtaaac gtcgttgttt aaatcacgca tccaggccgc cagcgccgcg ccaagaagcc
    34861 ccagagggat caccagaatg accgagaacg ggactgacca gctttcgtac agtgctgcaa
    34921 ggcagaggaa aaccaccaga atagaaactg catacagact taatgcctga ccgctggcca
    34981 gtttctcctg caatgacaaa ccactccagg cccaggttgt tcccgcagga agactgtttg
    35041 ccagttcctc catttttgtc attgcatcgc cggaactaaa gccagtcgcg ttttcgccct
    35101 gaatttcata ggctgccgag ccgttgtagc gcaccagtct ttccggtcca tacagccagc
    35161 gggtggtcgc aaaagcagag aatggtgtca tggcgttatc gctgccgcgc acaaaccatt
    35221 tacctaagtc tgacggcgcg gaacgaaatt cgctgtcgcc ctgaatgtag acttttttca
    35281 cacgaccacg gtcaataaag tcattcacat aagtaccgcc ccacgcactg gacagggtgt
    35341 cggtgacatc attcaaactc aaccctaatg acaccgcttt attactatca atatctacct
    35401 gcaattgcgg catttgtggc aaatcattgg cgcgtacaga atgtaactcc ggactttgat
    35461 tcgctttttc tatcaactga ttacgcattt gcagcagtgt ttcacgatcg gttccaccgt
    35521 tagctaacaa ttcaaacgta aaaccattgc tttggcccag cccatcaacg gctggcggcg
    35581 tcatcgcaaa taccgtggca tcacgaattg tgcccagctc tttggttgcc cgtagggcga
    35641 tagcctgggc ggtgttttct gcccctttac gttgagacca gtttttcaac gaaacaaacg
    35701 ccatcccggt gttctgtccg ctaccgctaa aactgaaacc atcaacggta aagatgacat
    35761 cggtatttgc tttctcgtta atcaggaacc agtcaacaat ctggcgattc acttctgctg
    35821 tacgggcagc ggtcgccccg gccggcagcg tgtactgcac catgatttcg ccctggtctt
    35881 cagtgggtaa gaaactgccc ggcagtttcc acatcatcag ggccattcca ccacccagca
    35941 cgacataaag ccccatcgtt cgggctgcac ggcgcaggac ataaattacg cctcgctgat
    36001 atttatcttc agtacggcgg taaaagcggt taaatgcgcc gaaaaagcct tttttatgtg
    36061 gtggaacatg ctggaggacg gaaccgcaca gggcgggagt caaggtcaat gccaccacca
    36121 cggaaagcag cattgcggag atgatggtga tggagaactg acgataaatt acccccgtag
    36181 atcccccaaa gaaggccatc ggcaggaata cggctgacaa caccagcgca atggcaacca
    36241 gtgcgccaga aatctcgccc attgattttt ccgtggcttc acgcgcgggc aacccttcgt
    36301 cacgcataat gcgttcgacg ttctctacca ccacaatggc atcgtccacc agcaagccga
    36361 tcgccagcac cattgcaaac aacgtcaggg tattaatcga atagccaaac aacgcaagaa
    36421 cgccgaatgt gcccagcaaa acgacgggga cagctaacgc cggaatcagt gtggcacgca
    36481 ggttttgcag gaataaatac atcacgcaaa ccaccaatac gatggcttca aacagcgtct
    36541 gaattacgtc ctctacggag attttaataa attcagtgct gtctttcggg tatgcgatgt
    36601 cgtatccctg cggcatgttc cgctggaatt cggcaatctt atccttgacc agcgtcgccg
    36661 tattcagcgc attcgcaccg ggcgaaagca ttacagcaac cccggcagac gggtgaccgt
    36721 taagtttgcc gatagcggta taatcttcac tgcccatctc aacgcgagcc acatctttta
    36781 tacgaacaac tgcgccgtct gactggcttt tcacgataat atttttgaac tgatccaccg
    36841 tctgcaagcg agactgcgta cgtaccgttg cggtcagttg ctgagtatcc ggtgaaggca
    36901 atgcccctat tttcccggca gtaacctgca cgttttgcgc ttcaatagca ctttgcacgt
    36961 ctgaaggcat cagcgagtaa gacgccagtt tggccgggtc aagccagatg cgcatagcgt
    37021 attccgcgcc aaagacttgc agactcccca caccttcaac acgcgccagc gggtcctgaa
    37081 cgttactgac cagccagtcg gcgatatccg aactggaggc tttgtcagtg gtgtcataca
    37141 cggcagcaat cagcaaaaag ttgctttgtg atttctccac cgtaacaccc gtttgctgca
    37201 cttcgctggg taggcgcgac tccgcctgct gaattttatt ctgcacctga acctgtgcag
    37261 tatctggatc ggtaccttgt tcaaaggtca cattaatact gaccgaacca tcagagctac
    37321 tggttgagct gaaatagagt aaattatcaa gcccagtgag ttgttgctcg ataacctgag
    37381 tcacgctgtt ctccagcgtt tcggcagaag caccagtata agtggctgaa attttaatgg
    37441 tcggcggtgc aacgtcagga tattgcgcga cgggcaatgt gcgaatagcc agaatccctg
    37501 ccaacataat caaaatggcg ataacccagg caaagaccgg gcggcgaaca aaaaagcgcg
    37561 aaaacatcag gcgttgcctc cattagcctg aacttcaaca gccttgacgg tctgccctga
    37621 agtgactttg gcagaacctt caacaatcag tcggtcgccg ttgtgcaggc cgttcagcac
    37681 cagccattta tcaccatacg tttctcccgt ttcgagcgtt cgctgctcta ctttattgtc
    37741 tttattcacc accagcgcag ttgcattgcc tttagcatcg cgcgtaacgc cctgttgagg
    37801 cgcgagaata gcgttttcca tcacgccttc atcgacacga gcgcgaacaa acattcccgg
    37861 taataactgc tgttgtggat tggggaaaat tgcccgtaat gtcaccgaac cggtagactc
    37921 atccaccgcg acttcggtga gttccagacg ccctttttcg ctgtaggttg tgccatcttc
    37981 cagaataagt gagacgctca tggtgtcact gttggtcgcc agtgactgtt tacgcagacg
    38041 taataaatcg acactggagc gagtgaggtc gacatacatt gtatccagac cacgaatagt
    38101 cgttaacgct gtatcctgcg acgcggtcac cagtgcgcca ggggttaccg acgaaatacc
    38161 aatacgcccc gaaatcggtg cggttaccgt ggtccagtca agattaatgc gcgcagtttc
    38221 cagtgcggct tttttcgcct ctacactggc tttatcttgt gcacaggtag actgagcatc
    38281 atcagcatcc tgttgtgaaa caccattctc tttcaccagt cgggcataac gctgcgcttt
    38341 ctggcaatcg gcttttacca gtgcctgtgc ttgttgtaat gctgcccggg cttcattcca
    38401 tgcagcctga taactggccg catcaatctg gtagagaggc tgtccagcct tgaccagatc
    38461 accttcctta aataagcgtt tctggataat tcccccaacc tgcggacgta cttcagcact
    38521 gagcgcagca ctggtgcgtc cggttaattc actgactacc gaaaccggtt gagatttgag
    38581 cgtaacaacg ccgacttctg tttctgacgg ggatgaattg ttactttgcg tattatcaca
    38641 acccgataag agcagcattg ccactacaga tgttgctata tacttcatta ttttctccag
    38701 ggtgagaaat accctcctca ttgaaattaa tgaggatatg tcagggatat tcagttataa
    38761 cgaacccgca tcgcgaaatg aatttcgcaa tgaaataagg gggattaata tcccccgcta
    38821 ttggttaatt ctgttcttat ttatttatca acatactaac aatatcctga tatatttcct
    38881 gcagatgttg tggatcgctt ttttgcggtg ttaaacgacg ataaaccgta ccttccatca
    38941 tggtagcaaa cacttcaacg cagcaacgaa tgtgctcgtc gctaagatgg ggatgcattc
    39001 ttttcatatg tgcacaaccg ttagcgaaca ttctggcgtc agcttcttca agcattgcca
    39061 taacccgcgg gttgcgtgtg gcttctgcag ccacttccag catcagcgcg tcgtcgtcct
    39121 catttaagga tttacgcaag gcaaggactt ccgggaagtg atcggtacgc gcgtcaatgt
    39181 ccatttgggc aatacgaaag tcgatgatac ggcgaaccat ctcttcaata atggcgtctt
    39241 tgttggcgaa gtaacgatat atctgaccca cgctaagctg agcttcgctg gcaatttgcg
    39301 acatactggc ggcatgaaat ccgcagcgac gaaaacaccg acgggcggcg ttaatgattt
    39361 catcctgacg ctgtttgtgg cgtatctcca gcttgctggt catttctgtc tcctggccta
    39421 tcacttggtg agaatgatca ttctcatttt tgctaattat acgtttctcc aaagtaatgg
    39481 aaagtatttt tatcttattc atgatgcata ttctgataat gaagagattg tggagattct
    39541 gtgaagatag ttgacaatat taagttggag tcaattcatc agaaagacag cgacttgttg
    39601 tcgccagttc tgttttcatc attaaactta taggagctaa tacgaatagt ttcaaagtca
    39661 gattctataa gatatttccc caacctcccc cttgcaggcg tcaggagtag ataacgcaaa
    39721 atgctctgta ccgcagcttc tctccgctac agaaagcgca actatagcca cccacagcca
    39781 ggttgattat aataaccgtt tatcagttcg tactgtttac taaaacgacg aatcgcctga
    39841 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
    39901 gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta tcgctcaggc
    39961 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
    40021 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgccga
    40081 agataccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
    40141 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
    40201 cgttactcgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
    40261 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
    40321 tggcgctgag cgcgctatcc cgaattacaa cgttatgggt ctggcaaaag cgtctctgga
    40381 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
    40441 ctctgctggt ccgatccgta ctctggcggc ttccggtatc aaagacttcc gcaaaatgct
    40501 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
    40561 ctccgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
    40621 tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ctgaaataat cgttctgttg
    40681 gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt atacctttct gatatttgtt
    40741 atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt tcctttacga
    40801 ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt atcatgaaac
    40861 gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg caaaggtcag
    40921 cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggccccct tcctttcatg
    40981 gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gacccgctta ccgtgactcg
    41041 ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg ctcaggtttg
    41101 cggcgtccaa cggttatgta actactattc tgcgcgactt acgccgctcc ccgggcctga
    41161 ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc gccaactggc
    41221 tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg ttggtcttac
    41281 tgtctgtgac tgtgtgatca ttaaccaaat cattggtttt attggctttc aggcgcggac
    41341 cattgcgaca tttcaggctt atctcgggca tccagtacgc tggttacccg ggctggagat
    41401 acaaaactac gccgacgcgt cactgtttgc tgatgaatca ttacgctggc gaagcagcta
    41461 tgaggtggaa aaactccctg aagagcacac aaaaagttca actgcagaac tttgccaact
    41521 ggccaacaca ctctctctcc accctatttc actttccctt ctcgaaaagt tgttaaacag
    41581 cacacgggtc aatacacagc cggataatca gcttgcggcg ttgttatgcg cgcggataaa
    41641 tggcagtcct gcttgttttg ccgcctgtat gaattcatca aatgaatata aaaaaatcag
    41701 cccccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc attctgttga
    41761 gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct ttagcgccgc
    41821 ccagttcagc ccattactcg aacacgaaaa atcatcaacg cagattatta atctgctagt
    41881 atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg agacatatta
    41941 accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat
    42001 agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa ctgcattccc
    42061 agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc ttcctggaag
    42121 tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg
    42181 accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag ccagaagaac
    42241 tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg
    42301 ccatcgttcc tgaccatcca ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc
    42361 tgaaccacga gtttaaagaa ggtgactggg cggttgccga aatgcgccgt catccgctga
    42421 aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt gacgatcact
    42481 ttgtaccgtg gtgggttacc cttgcgcgtc ataatctgga aaaagaagca ccagacggcg
    42541 ttgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgactgcg ctggattttg
    42601 tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgcc aaggcgttac
    42661 cggatggcaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg attgctgaag
    42721 gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg cctggcttca
    42781 acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc gccaatgaag
    42841 tccgcccggt actggcatgc cgcatgacgc tctccactga tggcaccatt gaagataata
    42901 tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac caggtttctg
    42961 actggctgga aaataccggt gactggcagc ctgaaagcga agcaattgcc gaacaagtcc
    43021 gtttgctagc acaaatttgc caacgccgcg gcgagtggcg tcataaccac gcactggtgt
    43081 ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg
    43141 tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca
    43201 tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac gtgcatatgg
    43261 gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg
    43321 tcgatgctga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt gaactggatg
    43381 cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt gctgaaatta
    43441 gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccact tggacttcgc
    43501 cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt atcaaaggcg
    43561 aaactgcgac gcgtccacag gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca
    43621 accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg aaagacaaag
    43681 ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg
    43741 ttcgtttggt tgataacggc gctatcgcct ttattccagc acctttctta cacgctgtgc
    43801 gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt gaaacggctt
    43861 acaaagtgac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa acccgcagca
    43921 ttattgcgcg cccagtcgcg taatctcctt tcacggccca ttcctcatgg atgggccgtt
    43981 tatttccccg ctcaaccttc gtcatatccc ggcagatttt taactgactt tcgtttgaaa
    44041 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat gtgtttagcc
    44101 gccgttaatt tcatgtaagt catgttgttc cttttattta actgcggact ccgctgttaa
    44161 ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca actttctcgg
    44221 atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct tttctaccac
    44281 cgaaatcaca gaacccgatc gtattttgca actatctgcc gaacaggctg ctcgcatcag
    44341 ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg aaagcgatct
    44401 ttctgtgcat ctggtaggac gaaaaatcaa taaacgagaa tggggaggca acgcgtctgc
    44461 atggcatgac acaccggcgg ttgctcgtga tttatcacat gggctttcct ttgctgagca
    44521 ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga atatccagcg
    44581 ctttaatcgg ttatgtgaag attacacagg cttgaaagaa cacgacgtca ttgggcaaag
    44641 cgtgtttaaa ctgtttatga gtcgtcgtga agctgcggca tccaggcgca ataaccgtgt
    44701 attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac gtaaaggcca
    44761 gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa acgagatttt
    44821 tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggagc gactgcgtat
    44881 tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc aggagttaat
    44941 cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc ttgatttgga
    45001 taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt tattacgcga
    45061 cgtgtcattg gctattttaa gctgtctcga acatgaccag gtattggcgc gtccaggtgg
    45121 cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag caatggcatc
    45181 acgaattttg acccgcttac ggctcccctt tcgcattggt ttaattgaag tttataccag
    45241 ctgttcagta ggtatttcac tctctcccga acatggttcg gacagcgcgg ctattattcg
    45301 tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt
    45361 taccccagaa atgaatcaga gggtatttga atatctctgg ctggatacca acttgcgtaa
    45421 agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggtga
    45481 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    45541 gttggacttc atttcctacg ccgaagaatc agggctaatt gtgcctttag gccgttgggt
    45601 gattctcgat gtcgtacgcc aggtagcgaa gtggcgggat aaaggcatta acctgcgagt
    45661 agcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    45721 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cggagagctg
    45781 cctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc gattaggtgc
    45841 gcaagttcat ctggacgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    45901 tccgatcgat gccatcaaac ttgaccaggt ttttgttcgg gatattcaca aacagcctgt
    45961 ctcgcaatca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    46021 cgccgaaggt gtagaaagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    46081 gcgacaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    46141 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat attccgtcat
    46201 tatatatcgt attcccggat gataattagc cgttgctgtt aaacccttct cttttagcgg
    46261 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    46321 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    46381 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    46441 tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt gccttcaggt
    46501 tttcctattt gaggatgccg gcgcgtcagt atttttttat ttagtattat aacgttataa
    46561 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
    46621 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
    46681 atctcaacac cctcgaaaaa ctgagttacc tccgacgtgc acatggtttt gcagtttcgc
    46741 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
    46801 ccgagtttgc cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggtagca
    46861 gcaatgccct gctcgcccgg actctgggcg agcagaagaa aaatgtcact atcatcacgg
    46921 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg
    46981 gcgtgtacca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccaac
    47041 aggtgcattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actgggttta
    47101 ccggtcgcga tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa gagtgcgagg
    47161 cgatagtcct gaccgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggccccg
    47221 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
    47281 ttgaacaaag taaactcacc gttcacgtcg ttgacattta accatttccc ttctcta
//
LOCUS       DAJOTB010000018        46723 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJOTB010000018 DAJOTB010000000
VERSION     DAJOTB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.018"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            74..706
                     /gene="comR"
                     /locus_tag="OHN28_001031"
     CDS             74..706
                     /gene="comR"
                     /locus_tag="OHN28_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCQ2296467.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(768..1307)
                     /locus_tag="OHN28_001032"
     CDS             complement(768..1307)
                     /locus_tag="OHN28_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2296468.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1534..2838)
                     /gene="ndh"
                     /locus_tag="OHN28_001033"
     CDS             complement(1534..2838)
                     /gene="ndh"
                     /locus_tag="OHN28_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCQ2296469.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3237..3779)
                     /locus_tag="OHN28_001034"
     CDS             complement(3237..3779)
                     /locus_tag="OHN28_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2296470.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3802..4827)
                     /gene="nagZ"
                     /locus_tag="OHN28_001035"
     CDS             complement(3802..4827)
                     /gene="nagZ"
                     /locus_tag="OHN28_001035"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCQ2296471.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4838..5662)
                     /gene="thiK"
                     /locus_tag="OHN28_001036"
     CDS             complement(4838..5662)
                     /gene="thiK"
                     /locus_tag="OHN28_001036"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCQ2296472.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5643..6284)
                     /gene="lpoB"
                     /locus_tag="OHN28_001037"
     CDS             complement(5643..6284)
                     /gene="lpoB"
                     /locus_tag="OHN28_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCQ2296473.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6298..6675)
                     /locus_tag="OHN28_001038"
     CDS             complement(6298..6675)
                     /locus_tag="OHN28_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCQ2296474.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6678..7037)
                     /gene="hinT"
                     /locus_tag="OHN28_001039"
     CDS             complement(6678..7037)
                     /gene="hinT"
                     /locus_tag="OHN28_001039"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCQ2296475.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7371..9560
                     /gene="fhuE"
                     /locus_tag="OHN28_001040"
     CDS             7371..9560
                     /gene="fhuE"
                     /locus_tag="OHN28_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCQ2296476.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGTYQF"
     gene            complement(9620..11053)
                     /gene="ptsG"
                     /locus_tag="OHN28_001041"
     CDS             complement(9620..11053)
                     /gene="ptsG"
                     /locus_tag="OHN28_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCQ2296477.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11348..12145)
                     /locus_tag="OHN28_001042"
     CDS             complement(11348..12145)
                     /locus_tag="OHN28_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2296478.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12156..13160)
                     /gene="holB"
                     /locus_tag="OHN28_001043"
     CDS             complement(12156..13160)
                     /gene="holB"
                     /locus_tag="OHN28_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCQ2296479.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13157..13798)
                     /locus_tag="OHN28_001044"
     CDS             complement(13157..13798)
                     /locus_tag="OHN28_001044"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCQ2296480.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13788..14810)
                     /gene="yceG"
                     /locus_tag="OHN28_001045"
     CDS             complement(13788..14810)
                     /gene="yceG"
                     /locus_tag="OHN28_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCQ2296481.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14813..15622)
                     /gene="pabC"
                     /locus_tag="OHN28_001046"
     CDS             complement(14813..15622)
                     /gene="pabC"
                     /locus_tag="OHN28_001046"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCQ2296482.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15742..16983)
                     /gene="fabF"
                     /locus_tag="OHN28_001047"
     CDS             complement(15742..16983)
                     /gene="fabF"
                     /locus_tag="OHN28_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCQ2296483.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17071..17307)
                     /gene="acpP"
                     /locus_tag="OHN28_001048"
     CDS             complement(17071..17307)
                     /gene="acpP"
                     /locus_tag="OHN28_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2296484.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17518..18252)
                     /gene="fabG"
                     /locus_tag="OHN28_001049"
     CDS             complement(17518..18252)
                     /gene="fabG"
                     /locus_tag="OHN28_001049"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCQ2296485.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18265..19194)
                     /gene="fabD"
                     /locus_tag="OHN28_001050"
     CDS             complement(18265..19194)
                     /gene="fabD"
                     /locus_tag="OHN28_001050"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCQ2296486.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19210..20163)
                     /gene="fabH"
                     /locus_tag="OHN28_001051"
     CDS             complement(19210..20163)
                     /gene="fabH"
                     /locus_tag="OHN28_001051"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCQ2296487.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20231..21301)
                     /gene="plsX"
                     /locus_tag="OHN28_001052"
     CDS             complement(20231..21301)
                     /gene="plsX"
                     /locus_tag="OHN28_001052"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCQ2296488.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21382..21555)
                     /gene="rpmF"
                     /locus_tag="OHN28_001053"
     CDS             complement(21382..21555)
                     /gene="rpmF"
                     /locus_tag="OHN28_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCQ2296489.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21607..22128)
                     /gene="yceD"
                     /locus_tag="OHN28_001054"
     CDS             complement(21607..22128)
                     /gene="yceD"
                     /locus_tag="OHN28_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCQ2296490.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22327..22911
                     /gene="yceF"
                     /locus_tag="OHN28_001055"
     CDS             22327..22911
                     /gene="yceF"
                     /locus_tag="OHN28_001055"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCQ2296491.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23023..23982)
                     /gene="rluC"
                     /locus_tag="OHN28_001056"
     CDS             complement(23023..23982)
                     /gene="rluC"
                     /locus_tag="OHN28_001056"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCQ2296492.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24555..27740
                     /gene="rne"
                     /locus_tag="OHN28_001057"
     CDS             24555..27740
                     /gene="rne"
                     /locus_tag="OHN28_001057"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCQ2296493.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27935..28888)
                     /gene="flgL"
                     /locus_tag="OHN28_001058"
     CDS             complement(27935..28888)
                     /gene="flgL"
                     /locus_tag="OHN28_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCQ2296494.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28900..30543)
                     /gene="flgK"
                     /locus_tag="OHN28_001059"
     CDS             complement(28900..30543)
                     /gene="flgK"
                     /locus_tag="OHN28_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCQ2296495.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30609..31550)
                     /gene="flgJ"
                     /locus_tag="OHN28_001060"
     CDS             complement(30609..31550)
                     /gene="flgJ"
                     /locus_tag="OHN28_001060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCQ2296496.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31550..32647)
                     /gene="flgI"
                     /locus_tag="OHN28_001061"
     CDS             complement(31550..32647)
                     /gene="flgI"
                     /locus_tag="OHN28_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCQ2296497.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32659..33357)
                     /gene="flgH"
                     /locus_tag="OHN28_001062"
     CDS             complement(32659..33357)
                     /gene="flgH"
                     /locus_tag="OHN28_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCQ2296498.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33411..34193)
                     /gene="flgG"
                     /locus_tag="OHN28_001063"
     CDS             complement(33411..34193)
                     /gene="flgG"
                     /locus_tag="OHN28_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCQ2296499.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34365..35120)
                     /gene="flgF"
                     /locus_tag="OHN28_001064"
     CDS             complement(34365..35120)
                     /gene="flgF"
                     /locus_tag="OHN28_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCQ2296500.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35140..36345)
                     /gene="flgE"
                     /locus_tag="OHN28_001065"
     CDS             complement(35140..36345)
                     /gene="flgE"
                     /locus_tag="OHN28_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCQ2296501.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWDVYTLDSSDP
                     TGTANPATTLVFNANGVLTSDPTKDITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36370..37065)
                     /gene="flgD"
                     /locus_tag="OHN28_001066"
     CDS             complement(36370..37065)
                     /gene="flgD"
                     /locus_tag="OHN28_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCQ2296502.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37077..37481)
                     /gene="flgC"
                     /locus_tag="OHN28_001067"
     CDS             complement(37077..37481)
                     /gene="flgC"
                     /locus_tag="OHN28_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCQ2296503.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37485..37901)
                     /gene="flgB"
                     /locus_tag="OHN28_001068"
     CDS             complement(37485..37901)
                     /gene="flgB"
                     /locus_tag="OHN28_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCQ2296504.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38056..38715
                     /gene="flgA"
                     /locus_tag="OHN28_001069"
     CDS             38056..38715
                     /gene="flgA"
                     /locus_tag="OHN28_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCQ2296505.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            38791..39084
                     /gene="flgM"
                     /locus_tag="OHN28_001070"
     CDS             38791..39084
                     /gene="flgM"
                     /locus_tag="OHN28_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCQ2296506.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39089..39505
                     /gene="flgN"
                     /locus_tag="OHN28_001071"
     CDS             39089..39505
                     /gene="flgN"
                     /locus_tag="OHN28_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCQ2296507.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(39545..41080)
                     /gene="murJ"
                     /locus_tag="OHN28_001072"
     CDS             complement(39545..41080)
                     /gene="murJ"
                     /locus_tag="OHN28_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCQ2296508.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41190..42113)
                     /locus_tag="OHN28_001073"
     CDS             complement(41190..42113)
                     /locus_tag="OHN28_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2296509.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42115..42762)
                     /locus_tag="OHN28_001074"
     CDS             complement(42115..42762)
                     /locus_tag="OHN28_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCQ2296510.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42773..43357)
                     /gene="rimJ"
                     /locus_tag="OHN28_001075"
     CDS             complement(42773..43357)
                     /gene="rimJ"
                     /locus_tag="OHN28_001075"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2296511.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43593..44801
                     /gene="mdtH"
                     /locus_tag="OHN28_001076"
     CDS             43593..44801
                     /gene="mdtH"
                     /locus_tag="OHN28_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCQ2296512.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44865..45512
                     /gene="grxB"
                     /locus_tag="OHN28_001077"
     CDS             44865..45512
                     /gene="grxB"
                     /locus_tag="OHN28_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCQ2296513.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45646..46206
                     /locus_tag="OHN28_001078"
     CDS             45646..46206
                     /locus_tag="OHN28_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2296514.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46312..>46723
                     /gene="pyrC"
                     /locus_tag="OHN28_001079"
     CDS             46312..>46723
                     /gene="pyrC"
                     /locus_tag="OHN28_001079"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCQ2296515.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPL"
BASE COUNT        11289 a        12650 c        11610 g        11174 t
ORIGIN      
        1 ttctggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
       61 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc
      121 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcagcacgg
      181 ttatgaagcg acttctcttg cggacctcgt tgaagcgacc ggagctaaag cgcccacgct
      241 atacgcggaa tttaccaaca aagaggggtt attccgcgcc gtactcgacc actatatcga
      301 tcgttttgcc gccaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
      361 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ctccggctgg
      421 ctgcttcatg atcaacaact gcaccaccct ctccccggat tcaggagata tcgccaatac
      481 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
      541 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
      601 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcactggaaa aactgatgca
      661 gattgcccga acgactttgc gtttatggcc ggaactgctg aaataggtcg caaaaaaagc
      721 ccctcagcct gagcgaatga ggggctaaat tgcagagtac agccttgtta tactttgtta
      781 ttcaaaatca gctgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
      841 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
      901 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
      961 agagagccct ggatctggtt acctgcatat ccacccccca gcgcccccac aacagtggcg
     1021 acatctttac cgcgaccacc tccaaactga tgcccaatca cgccgccagc aacagcgccg
     1081 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtaacg
     1141 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
     1201 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc
     1261 gcagctgcga caccaatccc gatacccgcc aacattgact tattcacggg aacacctcct
     1321 tctttgtagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
     1381 aaaagcgcgg gaatggctgg aaatactcag ggattcggag gagtctgaag gaatgcctga
     1441 tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
     1501 ggcgtttatg ccccatccgc cagtacaagt cgattaatgc aacttcaaac gtggacggat
     1561 aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc
     1621 aatctggtgc attcggtaca gcgagatgta tacaaagcgc gcaattcgtc cttcaatcat
     1681 cattgagccg cgcgtcaggt tacccatcag gctaccgact gtggaaaagt tcgacagcga
     1741 taccagcgaa ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
     1801 cagaatgttg ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac
     1861 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc
     1921 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
     1981 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
     2041 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
     2101 ttgcgtcagc acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg
     2161 tggcggcaat gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc
     2221 gttggtcaga cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc
     2281 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc
     2341 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
     2401 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggtgtat tgaaatcgtt
     2461 agaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
     2521 cagttcacct ttctcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
     2581 atcaattacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
     2641 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
     2701 caagtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
     2761 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
     2821 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
     2881 tattaacata gctcttaatt aacaacaggt tatagcaatg aaaatgttct tcgctattga
     2941 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg
     3001 aaaagatagg cgactttacc gggtgagatc aaaaaacaga gtttgttgtt cattatttga
     3061 gctgatttgt gagaatgata tagagtgagg tgtgtatgga gggcgttcgg taggcctgat
     3121 aagaggcgca tcaggcattg atgctcgact gtcggatgcg gcggaacgcc ttatccaacc
     3181 cacagaatca ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac
     3241 ccgagggttt tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc
     3301 gtctgctctt cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg
     3361 ctgttaagcg cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttctca
     3421 cggaagttgg tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttc
     3481 ccttccatgt tctcataagg gaacaaattg gggttgaaga tcacctggcg gatatcacag
     3541 agaaaaccaa tccgctcagc ccaataaccg cccagaccaa caccacaaat gagcgggcgc
     3601 tcgtcgacgt tcagttgcaa cattttgtcc acttctttga gcaaatgctg catatcatgt
     3661 tttggatgcc gcgtactgta gcttattagc cgtacatccg ggtcaataaa ctgcaattgt
     3721 aagactttct cgtggttacc cggactgtta gagtcaaaac cgtgcaaata gataatcatc
     3781 gcttcctcac ataagccagg gttaatgacc tgccttctct tcctgccagc gttcatgtaa
     3841 ctgattcaga cgggcgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga
     3901 aaatgaacct ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc
     3961 taacacgctg acggcccctt tacgattatt gcagaccagg atcatatcgc aacctgcatc
     4021 cagtgatgcc tgtccacgct cggcataact gcccataatc gctgcgcctt ccatcgataa
     4081 atcgtcagag aaaatcacgc cgtcaaaacc caattcctga cgcaaaacgg ttttcagcca
     4141 gtagggagaa ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat
     4201 aatggcgtcg agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg
     4261 aatctccgct tgcggacgtg ggtcgcacgg tgtttctttg tgtgagtctg ccgttactgc
     4321 accgtgacct gggaagtgtt tcccggtagt tttcattccg gcttcatgca taccatcaat
     4381 aaaccggctg gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat
     4441 cgccgcgctg atatgcccga cgtccagcac tggcgcaaag ctgatatcaa tatccatagc
     4501 aatcatttcg ctggccatca accaacctgc ctcttgcgcc agttttccgc cttcttccat
     4561 tcccaacagc gcagcgaatg attgtgccgc tggcaagcgg gtaaaacctt cacgaaaacg
     4621 ctgcacgcgt ccaccttcct gatctaccgc caccaccaga tgatttcgcg atgctgcgcg
     4681 gatctggcgc accagttcac gtaactgggc aggatcatga tagtttcgcg taaagagaat
     4741 cagccctccc accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc
     4801 ttcgacatcc aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc
     4861 gccaggtgtc atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact
     4921 caaaccaccc tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata
     4981 attgcgccgg ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cgatgctggt
     5041 cagtattttc cacccacacc gccgccagtt ccagcgcgat atcaccatct ccggcatact
     5101 cccagtcgat gagttttaac cctgacgcgc tatgcactaa atttccggcg tggacatcca
     5161 tatgcaatgg acttaagcgt aaaagccgtg gttcccgcgc tttgcgcaga cgttttaaca
     5221 ttcgcagcca acccactgtc cgccgcgccg gatcgctttg ctgccagtac agttccagta
     5281 acggcaacag cgttattcgc cagccaaaac gcggttgttg atgtagataa tacagcaagc
     5341 ctgccagttc gttggtatcc ggcaaatacg tttttacctc gccgggcaga tagtcaacca
     5401 ccatccagtc acggagatat aaatgcggct tcggtgcaat gcatgcgggt agttgtgata
     5461 aagcccgata ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca
     5521 caacaaaacg ctggccctga tgttcaatga gaaaactccc gccactaagc ccactattaa
     5581 acgtcgtgac gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgaggg
     5641 gattattgct gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc
     5701 atcagctgca tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg
     5761 taatgagcgc caacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc
     5821 tgcggcgaca aacctaattg ttgcttagcc atcgacagct gctgggcgga aaccagggta
     5881 aatttaccgt tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcaac
     5941 gaaccgttag tacggttgtt aacgctatca accagcagga cgctacccgc agtcacgcca
     6001 tcagccccaa gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga
     6061 atatgcggcg caggcggtgc agtttgatct tcgtgctcaa ttgggcctgg ctgctgcggt
     6121 atcgtcggca ccgatggcac tgtggggaca ggctgttgtg gctcggttgg ttgttccggc
     6181 gctggtttca cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcaagaaac
     6241 atcgccagcg cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta
     6301 caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt
     6361 gaccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag
     6421 ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt
     6481 ataaagcgtt gaggatgctg aaggttgaat atcagatgtc gaaaggacgg gcttttctgc
     6541 actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg
     6601 aatttccgga tgtgaacgac agcccaccag cactaacaac gccagagaca ccagcccaaa
     6661 gcatcctttt ctcatcgtta cagaccttta tgcgccagca ttggtcccag cggacggcca
     6721 cccaacaagt gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtgttcatg
     6781 atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg
     6841 atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt cgttcacagt cggaatgagg
     6901 atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt
     6961 accagatcat cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat
     7021 atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac
     7081 gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt caaccttaac
     7141 tcacgatttc ttaagcaaaa aaccgttttg ctgccgtata tatcgccatt attcccattt
     7201 ctgcctgcga atgcgctgaa aagacatttt ctgtttggtt tacacaccaa atatgaatgc
     7261 gtatatttct catttgcatt tacatacaaa attattcgca cactaaatat aaccgctcat
     7321 caccctgctc cggcatgagc ctgacgccaa cataaaccaa gagatttcaa atgctttcga
     7381 cacaatttaa cagggataat caacatcaag ccatcatcaa accgtcactc cttgccggtt
     7441 gcatagcact ggcactatta ccttccgccg cttttgctgc accagccact gaagaaacgg
     7501 tgattgttga gggttcagcc acagccccag atgatggcga aaatgattac aacgtaacgt
     7561 ctacctctgc gggtacaaaa atgcagatga ctcaacgtga tattcctcag tcggtcacta
     7621 ttgttagcca gcagcggatg gaagatcagc agttacaaac gctgggcgaa gtgatggaaa
     7681 acacgctggg gatcagcaaa agtcaggcgg attccgatcg tgctctttat tattcccgcg
     7741 gattccagat cgataactat atggttgatg gtatccccac ctattttgaa tcgcgctgga
     7801 atctgggcga cgcactttct gatatggcac tgtttgaacg cgtagaagta gtgcgtggcg
     7861 cgacaggcct catgaccggg acgggtaatc catctgcggc aattaatatg gttcgaaaac
     7921 acgcgaccag tcgtgaattt aaaggcgatg tctcggcgga atacggtagc tggaacaaag
     7981 aacggtatgt ggcggattta caaagcccac tcaccgaaga cggtaaaatc cgcgcacgaa
     8041 ttgtcggcgg ctaccagaat aacgactcat ggctggaccg ctacaacagt gaaaagacct
     8101 tcttctccgg cattgtcgat gctgatttag gcgatcttac gatgctttca gccggttacg
     8161 aatatcagcg tattgatgtt aatagcccaa cctggggcgg tttaccgcgc tggaatactg
     8221 atggcagcag caacagttac gatcgcgcac gcagtaccgc acctgactgg gcgtacaacg
     8281 ataaagagat caacaaggtc tttatgaccc tgaagcagcg gtttgctgat acctggcaag
     8341 cgacgctgaa tgccacccac tctgaagtcg aatttgacag caaaatgatg tatgtcgatg
     8401 cctatgtaaa caaagcggat ggtatgctgg ttgggccata cagtaattat ggccctggtt
     8461 ttgattatgt cggaggcacc ggttggaaca gcggcaaacg taaagttgat gcgctggatc
     8521 tgttcgctga cggtagttat gaattgtttg gtcgtcagca caatttaatg tttggtggca
     8581 gttacagcaa acaaaacaat cgttacttca gttcatgggc caacattttc ccggatgaaa
     8641 ttggcagttt ctacaacttt aatggcaatt tcccacaaac cgacaggtca ccacagagtc
     8701 tggcgcagga cgataccaca catatgaaat cgttatatgc tgccactcgt gtcacccttg
     8761 ccgatccgct gcatctgatc ctcggcgcac gttataccaa gtggcgggtt gatacgctga
     8821 cttacagcat ggagaaaaac cacaccacgc cttatgctgg actggtgttt gacatcaatg
     8881 ataactggtc gacctacgcc agctatacct ctatttttca gccgcaaaat gatcgtgaca
     8941 gttcaggcaa atatctggca ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg
     9001 actggatgaa tagccgtctg accaccacgt tagccatctt ccgtattgag caggataatg
     9061 tcgctcagtc caccggtaca cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg
     9121 tggatgggac agtcagtaaa ggtgtggaat ttgaactcaa cggcgcaatt accgacaact
     9181 ggcagctgac gtttggcgca acgcgctata ttgcagagga taacgaagga aacgccgtta
     9241 atcctaatct gccacgcacc acggttaaaa tgttcaccag ctatcggttg cctgtcatgc
     9301 cagagttgac ggtcggcggt ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga
     9361 caccgtatgg caccttccgc gccgagcaag gtagctacgc gctggtggat ctcttcaccc
     9421 gctaccaggt gacgaaaaac ttctcgttac aggggaacgt caataacctg ttcgacaaaa
     9481 cctacgatac caacgtggaa ggttctattg tctacggcgc accgcgtaat ttaagcatta
     9541 ccggcacgta tcaattctga ataacgcctg taaaaaaggc agccatctgg ctgccttagt
     9601 ctccccaacg tcttacggat tagtggttac ggatgtactc atccatctcg gttttcaggt
     9661 tatcggattt agtaccgaaa atcgcctgaa caccagaacc agcaaccact acgcccgctg
     9721 cgcccagttt cttcaggcca gcctgatcca ctttagacac atcagcaacg ctgacgcgca
     9781 gacgggtaat acatgcgtcg aggttagtaa tgttttcttt accaccaaat gcagcaacca
     9841 gagccggtgc catttcgctg gtacctgtcg cttttgcgtc ttcagtcgcg tcttcacgac
     9901 ccggcgtttt cagatccagt gctttaatca gcacgcggaa gatggtgtag taaacaatgg
     9961 cataaccgat accgacgatc gggaacagcc acagtttgct gctgttacca gacagaacga
    10021 tgaagtcgat cagaccgtgc gagaacgacg taccgtcacg catccccaga agaatacaga
    10081 ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta caggatcggc gcaacgaaca
    10141 tgaaggagaa ctcgatcggc tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga
    10201 tcataatacc gcccactttc gcgcggtttt ctggtttagc agagtgccag atagcaattg
    10261 cggcagctgg cagaccgtac attttgaaca ggaagccacc agacagttta cccgcagtcg
    10321 ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt
    10381 attcaccaat ctgcatctgg aaaggtacgt tccagatgtg gtgcagacca aacggtacca
    10441 ggcaacgttc gatgaaaccg taaatgccaa acgcaactac cgggttctgg taagcagccc
    10501 actgagagaa ggtctggatt gcagagccaa taggcggcca aatgaaggac agcacaacgc
    10561 cagtaaagat ggcagtcagg cctgaaatga tcggcacaaa gcgtttaccg gcaaagaagc
    10621 caagatactc aggcagctta atacggtaga aacggttaaa catgtacgct gcgatcgcac
    10681 cggagataat ccccccgagt acgccagtat ccgccaggtg tttagaggcg atttcttcag
    10741 caggtaaatg cagtaccagt ggcgcaacca cggccatggt tttaaccatg atgccatagg
    10801 caacaactgc ggccagcgcg gatacgccat cgttattggt aaagccgagg gcgacaccga
    10861 tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg
    10921 atacaacggc gggcagccag ctgaaattcg cggaaccgac gcccagcaga atacctgcga
    10981 taggcagtac ggataccggc agcatcagcg atttaccgac cttttgcagg ttagcaaatg
    11041 cattcttaaa cataattgag agtgctcctg agtatgggtg ctttttttac gttctcacgc
    11101 gtggcaaggg gggagtgcct cgccgtgtac agggcatcta agcgcccttt atttattaca
    11161 cagagtaaaa taattcagtg ccgatttgtt tgacggctat cacgtttcaa cttcagatca
    11221 ttggagaagt tgcacatttt gacaaaacat atgtcacaat atttcaaacg tggttattca
    11281 ccgcccgatt ttcggcggtg aactttactc gttttagcca ttaattacga gctttaaaaa
    11341 aactcattca acggatggat tgaaggcggg aagcatcgat gtgaaacaga cgggcgaagt
    11401 tatcggtggt tacctgcgcc agttcttcaa cggcaacacc tttcaacaca gccatgtatt
    11461 ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc tcgatgcggt actggcgcaa
    11521 ggtaaggtga gtcagtttcc accagtaacc gatccagggg gacataacgc gcagcatcgc
    11581 gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat
    11641 ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat
    11701 ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc gcgggcgtca cgggtgtgga
    11761 cgataaccgg cttgttcagt tcacgaccaa tctggatatg atggatgaag gactcttgct
    11821 gacgtacttt agtttccggc gtgtaataat aatccagccc ggtttcaccc agcgctacaa
    11881 cgccctcttc tgccgccaga cgacgtaaat cttctacatc gtagggatca ttctggttga
    11941 gtggatgcac gccacaagaa aatacgacgt tgtcgcgttc gcctaccaga tcccgcatgt
    12001 gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg
    12061 ctttcgccag aacgtcatcc acgtccttat gcaaagattc ataatccaga ccatcgagat
    12121 ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa gatgaggaac cggtagcaca
    12181 acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg
    12241 ttgatacctg tgacagacat taactgttca cgaatgtggc aaacatcccc cagtatagcc
    12301 tgcaggcgcg agggagaaag atggtttgcc agttcgacga ccagccccgg cacatcaaca
    12361 ttggtcacgt gcgcagcgcc ataatggcgt tttagcgcat ccatcagcaa cgttgccagc
    12421 cagtgtaaac gcgccggagc ttgttcatta ttaagggccg ctagcagcga ataccagtcg
    12481 cccgattgca cgccatatgc cagcgcctga cacaatgttt cacgagcctg ccagttatct
    12541 ccctgaaaca acgccagtgc cgccccaggc gaaccggcgc ttaagcgcaa tgcggcaagt
    12601 agtgcatcct gtgacattgt cacttcgcgt gaaagccagg tcacggcgta ctgttccggc
    12661 ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg ttgccagtaa acgttctggc
    12721 tcgcgggtag caaggaaaaa ccacgtctct gctggtggct cttcaagcgt tttcagcaat
    12781 gcgttagccg cggcgtcggt tagtaaggca gcatcggtta cccagacaac tttcgcacca
    12841 cctaagcgtg cgtgctcatt cagcttttcg gtgacctcac gtaccgcatc aatgcccagc
    12901 gcatttttcc ctttttcggg agccagggtg tagtaatcgg gatgtgtgcc agcctgcatc
    12961 aactgacatc cacgacagtg accgcaactt ttgtggccct gcggttgttg gcagagtaaa
    13021 taacggctca gggcgtagat taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt
    13081 agcgcatggt gacctcttcc ggcctgatag ctggctacca gtttttcgaa atcaggtcgt
    13141 aaccatggat accatctcat gcgtccaact ccttcaccca attggtcacg gtagtgcgga
    13201 ttgcatccat cacggcctcc agcggctggg tggcatcaat ggtatgaatg cttttatctt
    13261 gtgctgccag ttccagatag cgggcgcggg tgcgattaaa gaaatcgaat gactcttgct
    13321 caatacgatc cagctcacca cgggcgcgag cacgttttaa gccgacctct ggggtgacat
    13381 caagataaag cgttaagtca gggcgaaaat ccccgagaac agcatcacgc agtgttgcca
    13441 gcatatgttg gtcaatacca cgtcctccac cctgataggc ttgagtggag agatcgtggc
    13501 gatcgccaat cacccaggtg ccgttagcca gcgcaggttt gatgaccgtt tctaccagtt
    13561 gaacgcgcgc ggcataaaac atcaaaactt cggctttatc ggtaatgact tcatcgccta
    13621 ccgatttgat atccagcacc aggcttctta acttttcggc aagttgcgta ccgccaggtt
    13681 cccgagtgaa aaccatgtcg cggataccca gttgctcaag cgtctcaacc accacgttac
    13741 gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac gatatactta ctgcgcattt
    13801 ttttccttaa gcactttcag ataatcctgc acagacttgt tatgactggc aagattggta
    13861 ttaaacgtgt gaccaccttt accatcggcc acaaaataga gatacggcgt ttttgccgga
    13921 tgcgcagcag ccttcagcga atccgccccc ggcgtcgcta tcgcacctgg cggcagaccg
    13981 gtaatggtat aggtgttata cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca
    14041 ttataacgct ctcccatccc gtaaatcacg gtcgggtcgg tctgcaggcg cataccaatg
    14101 cgtaaacggt tgataaatac tgaggcaacc tgatcgcgtt cactggcaac ggcggtttct
    14161 ttttcgataa ttgatgccat cgtcaccaac tggtttttat ctttataagg cagaccgtcc
    14221 gcacgccctt cccaggcgct atcgaccgct ttcaccattt tcttgtgcgc tcgtttgagt
    14281 aacgcgacat cggtggtatt ggcggtatac atccaggtgt ctggccagaa ccaaccttca
    14341 atccactccg ggttttccag ttcaagtgcc tgcgctacgg tggcgtactt atcgtcgctc
    14401 agcgtgtgct tgatatacgg agcctcacgc aattgcttga ggtagtcgct cagacgcatc
    14461 ccttctacca gtcgcagcgg gaactgtgct tctttaccgc tttccaacaa tttcagcatc
    14521 tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc
    14581 ggttcgatac gcagcagcca ttggaaaacc cgtgggcgat tgatgatctt atcggcataa
    14641 agctgttcac cgagcgccag acgtccggtc cctggcttca gggtaaatat cgtctcttct
    14701 ttgataagca atttgctgtc ggcaagatgg cgaaccttcc agacgcccac accagcggcg
    14761 atacccagta ccaccagcaa taacaagatt atcaataaca cttttttcat gactaattcg
    14821 ggcgctcaca aagtggggct aaatattcat ataacgttgc tgacgaaaag gagacatcgc
    14881 cacaggcacg tacgggcatc actggcatta acgcattaca aataaccatc tcatctgcct
    14941 gcaacgcctc ttccagagag gcttgcactt cgacaagctg ataagaggat tgtgccagca
    15001 aacggataca gaattgtcgc ataatgccgt taacacctgc ctgatccagt cgcggcgtat
    15061 aaactacgtt gcccttccgc cagaacaaat tagccgcaca gcattccgta acccaccctt
    15121 cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc aagatgagag cggatcaata
    15181 cttgctcaag ccgattaaga tgtttaatac ctgcaagatg aggattgcgc cccagccgca
    15241 ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg gtcgtaatgt gcaggataag
    15301 ccgtaacgga gagaatccgc gttgccggtc cactgttcaa tgtgctgtac cctcgcccgc
    15361 cactaccgcg actgatcacg actttcagta caccattttg ctgttctgct gccagcgttt
    15421 tcatctcttg ttcaagctga ggccagaagt cacaggaaat catcaaccgc tgacaagcat
    15481 cctgtagtcg ctggatatgc gccgataaca aactgacttt accgtcgata actctggcgg
    15541 tggtaaaaca accatcacca aactgcgttg cccgatcgct tactgccagc gattcctgct
    15601 tatgaccgtt aattaagaac ataccggctc cttatcagag aattgttagt gtggcagcat
    15661 gttcactacg gaacaagtcg gaataaaagc taagaaaaaa ggcccgcaag cggacctttt
    15721 ataagggtgg aaaatgacaa tttagatctt tttaaagatc aaagaaccat tagtgccacc
    15781 gaagccgaag gaattacaca gagtgtattc cattccgcta acctgacgcg cttcgtgcgg
    15841 tacgaaatcc aaatcgcaac cttcatccgg gttatccagg ttgatggttg gcggaacagc
    15901 ctgatcgcgc agcgcaagga tggagtagat agattctact gcacccgccg cacctaacag
    15961 gtgaccggtc atagatttcg tggagcttac caacacgcgg cttgcagctt caccgaagat
    16021 ggttttcacc gcctgcgctt cagctttatc gccagccggc gtagaagtac cgtgcgcgtt
    16081 aacgtagcca atctgactcg cttcaatgcc tgcatcacgc agagcatttg ccatcgccag
    16141 agctgcgcct gcgccatttt ctggcggtga cgtcatatga taagcatcgc tgctcatacc
    16201 aaagccgacg agttcagcgt aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc
    16261 aagtaccagc ataccggcac catcgcccag tacgaaacca tcacgctctt tatcccacgg
    16321 gcggctcgcc gcttgcgggt tatcattgcg ggtagataat gcgcgtgccg cgccaaaacc
    16381 accaacgccc agcggcgtac tggctttctc tgcgccacct gcaaccatca cgtcagcatc
    16441 gccatacgcg ataatacgcg cagcatggcc aatgttgtgc acgccggaag tacaggcggt
    16501 cgcgatagag atgcttgggc cacgcaggcc atacatgata gtcagatgac ctgccaccat
    16561 gttcacaatc gttgacggaa cgaagaatgg gctgatctta cgtggaccac cgttcatcag
    16621 agatgtgtgg ttttcttcga tcagtccgag gccgccaatc ccggagccaa ttgcggcacc
    16681 aatgcgggtt gcgttctctt ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc
    16741 agcgacaatt ccatattgaa tgaaggcatc catcttgcgc tgttctttgc gcgagataat
    16801 gtcctcacag ttaaaatcct ttactaagcc agcaaatttc gttgcatagg cgctagtatc
    16861 gaaatggtcg attaggctga tgccactctg accggcaagc agagctttcc aggtagactc
    16921 tacggtattg ccgacaggag acaacatgcc cagtccggtc acaactacac gacgcttaga
    16981 cacgtttgtc ctccagggag ggaaaaaatg attctagtgg gacaaaaaga taaaactcag
    17041 gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg tggccgttga tgtaatcaat
    17101 ggcagcctga acggtggtga ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc
    17161 ttcttccaga gccattacca gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac
    17221 gaaagaagca ttgttggtaa cttcttcctg cttaacgccc agctgttcgc cgataatttt
    17281 cttaacgcgt tcttcgatag tgctcatact cttaaatttc ctatcaaaac tcgctttcgc
    17341 gatggttttc gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta
    17401 ccacgatttt acgttatttt gaggccttac cccccaataa cgcaaatcat tttgcactaa
    17461 ttattactca taaccacgca gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca
    17521 gaccatgtac atcccgccgt tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc
    17581 ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg
    17641 aacctgcgcc aggatacccg cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc
    17701 aataaagccc ggagcaacaa cgtttacagt aataccgcgt gacgcaactt cgcgcgccag
    17761 tgatttactg aagccgatca agcccgcttt cgccgcagcg tagttggcct gaccgccatt
    17821 tcccatggta ccaaccacag aaccgatagt gataatacga ccatgacgct ttttcatcat
    17881 agcgcgcatt accgcttttg acagacggaa aacagatgaa aggttggttt cgataatatc
    17941 gttccactct tcatctttca ttcgcattaa caggttatca cgagtgatac cggcattatt
    18001 gaccaggata tccacttcac caaattctgc gcgaattttt tccagaacag attcgataga
    18061 tgccgggtcg gtcacattca acatcagacc tttgccgttg gcacctaaat aatcactgat
    18121 cgcctgagcg ccattttcac tggtcgcagt gccaataact ttcgcgccac gggctgcgag
    18181 cgtttcagca attgcgcggc caattccgcg gcttgcaccg gttaccagtg cgatttttcc
    18241 ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc
    18301 gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag taagcacttt
    18361 gcccgggccg acttcataga gatgttctac gccttgcgct gccatgtatt caacagactt
    18421 cgtccactga accgggttat acaactgacg taccagtgcg tcacgaatgg catcaccatt
    18481 ggtttcgcat ttcacatcaa cgttattcac aacaggaact gttggtgcgt taaaggtgat
    18541 tttcgctaat tctactgcca gtttgtcggc tgctggtttc atcagcgcac agtgagacgg
    18601 tacgctcact ggtagcggca gagcgcgttt tgcgcccgca gctttacaag cagcacccgc
    18661 acgctcaacc gcttctttat gaccggcaat aaccacctgt cccggagagt taaagtttac
    18721 cggagaaacg acctgacctt ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc
    18781 cagaccgatg attgccgcca tagcacccgt tccttccggt acggcttctt gcatgaactt
    18841 gccgcgcatc tcaaccagac gcaccgcatc agcgaaatca atcacaccag cgcaaaccag
    18901 cgcggagtat tcccccaggc tgtgaccggc catcattgcc ggtgctttac cgccctgctg
    18961 ctgccatacg cgatacagcg caacagatgc agtcaacagc gctggctggg tttgccaggt
    19021 tttattcagt tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc
    19081 cgcagaagct tcagcaaacg tttcttcgac aattggatag ctcgccgcca tatcagccag
    19141 cattccaacg gtttgagaac cctgtccagg gaacacaaat gcaaattgcg tcatgtttta
    19201 atccttatcc tagaaacgaa ccagcgcgga gccccaggtg aatccaccgc caaaggcttc
    19261 aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt acagcttcat ccagagcgca
    19321 cgggacagag gccgcagagg tattaccgtg gcgatccagc gtcaccacga cattgtccat
    19381 cgacatgccg agttttttcg ctgttgcact gataatacgc aggttagcct gatgcggaac
    19441 cagccagtcc agttgagaac ggtcaagatt attcgccgtc agcgtctcat caacgatgtg
    19501 cgccagttct gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca gatgaattga
    19561 attctctgga ttcacacggt cagcattagg cagcgtcagc aactcaccat agctaccgtc
    19621 ggcatgcaga tgggtggaga tgattcccgg ctcttcagag gcagccagca ccgcagcgcc
    19681 cgcgccatcg ccaaaaataa taatagtccc acgatctgtt ggatcgcagg tgcgcgccag
    19741 tacatcggaa ccgacgacca gagcatactt caccgcccca gatttcacgt attgatcggc
    19801 tacgcttaat gcataggtga aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc
    19861 tttaatgccc agcatgcttt gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga
    19921 agtcgttgcc acaacgatca ggccaatctg gtctttctca atgcccgcca tctcaattgc
    19981 gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg tttggcgcgg caatgtggcg
    20041 ttcgcggata ccggtacgag tgacaatcca ctcgtcagag gtgtccacca ttttttccaa
    20101 atcggcgttt gtccgcactt gttcgggcag atagctgcca gtaccaataa tcttcgtata
    20161 catgtacgct cagtcacttt tcggttatat accgtcactt gcaaactgcg agttcgctgg
    20221 cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc cagcagctca aaaccagctg
    20281 ggtatacaga ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc accgcctgca
    20341 ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc tgcaccatga ctttttatca
    20401 ccgtgccgcg caatcctaac agacaggcgc cgttatactg gtcggggttg aggtgactga
    20461 atcgcctcgt caggctcttt tgtagccaac gctttaataa cagtagccac cacgaccgtt
    20521 ttttcccttc accctgagat ttcagcagag aaaggaacat cctgacaaca ccttccatcg
    20581 tctttaatgt gacatttcct gtaaagccgt cacaaaccag cacatctgtc ttgccagtta
    20641 acaactcatt ggcttcaaga tagccgatat aattgataga agggattgtt ttaagcaccg
    20701 ctgaggcatc ccgaatactg tcgagaccct ttacttcttc ttcaccaata ttgagcaacg
    20761 ccacgcgagg attaggaatt tccaccacct cttcagccag aactgagccc ataatggcaa
    20821 attgcaccaa cattgtactg tcacaatcga cgttggcccc taaatcgagg accaccgttt
    20881 tgcccttttg ctgatgcggt aataccgtca ccagcgccgg acgctcaatc ccctccaggg
    20941 gcttgagtaa taattttgcc agccccatta gcgccccggt attaccggca ctgacacagg
    21001 cttgcgctcg accttctttc accagctcca gggccacgcg cattgaactc ccacgactgg
    21061 cgcggatagc ttgcgaaggc cgggcatcac tggcgataac tgactgcgca ggaataatct
    21121 gcagacgcga acgttgttca aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat
    21181 tgccgactaa aagaagagtg agttgcgaat tagaattcag tgcctgcaat gctgcaggca
    21241 ctgtcacgga agggccaaaa tcccctccca tgacatctaa cgccagggtt agacgtgtca
    21301 aggtatcgtc gccgcccggt ttggtctttc cccagttgcc tggggaaaat cctcactaag
    21361 cttcatcacg cagacgcgta attacttagc gatgaccttg cggccgcggt agtaaccgtc
    21421 ggcagtgatg tggtgacgca ggtgtttttc accagaagtt ttgtctacag acaggctggt
    21481 gactgcggtc agcgcgtcat gggaacgacg catgccacgt ttggaacggg ttggtttatt
    21541 ctgttgtacg gccatggacc ttactcctca attacggttt atccccgatc caacggggag
    21601 caccaattac ttacgcttta agctggctaa tacggcaaat gggtttggct tttgcgcttc
    21661 ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt cagaatcatg
    21721 caccggaact accggcaagg cgaggatgat ttcatcttca accattgcaa gcagatcgat
    21781 ttcaccgaat tcgttaacct caatcggttc atacgcttcc ggcagtgctt cagcctgttc
    21841 gtctgaacgc acaggactaa aacaatacgt tgtgtagacc tgatgagtaa acggcttccc
    21901 gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg
    21961 ttggttatcg atagcgaacg acatggagca ttccacatca ctgtccacac tgactacgga
    22021 ttcggcgacg cgctcaacct gatcaggagt atagataccc tggtaatcaa ggcgtttttg
    22081 agccgtacga accggatcga gagtcagggg taattttacc ttttgcatag ggcgcgcata
    22141 ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat
    22201 aattcgcaca cattgcgggt tactgcttta ttttcgttca gtgctgataa cgaacatcaa
    22261 cctgcaaaac gaagcgcata gtttaaaatg gctatcatca atacgctata tctgatggaa
    22321 aaaaatatgc ctgaacttat tttagcctcc acctcgccct ggcgtcgcgc tcttctggaa
    22381 aaactgcaaa tctcttttga atgtgcagca cccgaggtcg acgaaactcc acgcagcgac
    22441 gaatcaccgc gacagttggt gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca
    22501 cgttatccgg atcatttaat tattggttca gaccaggtat gtgttcttga tggcgaaatc
    22561 accggtaaac cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat
    22621 atcgtcacct tctatactgg actggcgttg tttaattcgg cgaatgggca tctacaaaca
    22681 gaagtggagc cttttgacgt ccatttccgt cacctgagcg aggcggagat tgataattac
    22741 gtgcgtaaag agcatcccct gcactgcgcg ggtagcttta agagtgaagg atttggcatt
    22801 acgctgtttg agcgtttaga ggggcgtgat cctaacacgc tggttggttt accgctaatc
    22861 gcgctgtgtc agatgttacg tcgggaagga aaaaacccgc tgatgggata gttttttgcg
    22921 ccggatgcgg ttacgtcgca tccggcaatc ggtgctggcg gcctgatgcg gcataaacgc
    22981 cttatcaggc caaccgccca ttatcatgct tatatgctcg tattagcgcg cgttacgcag
    23041 tttttgcaaa caacgcttca aaccatcatc catcggcgct tcgatacgca tcacctcacc
    23101 ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt
    23161 gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat
    23221 cggatgaccc gcatattgtg tatgcacacg gatctgatga gtacgccctg ttaccgggct
    23281 acaacgcacc agggtggcaa atgcatagcg ctcttccact ttaaagcgtg tttccgacgg
    23341 tttgccttcc tgactcacac gcacgatacg ttcgccgctt tgcagaatat ttttcagtaa
    23401 cggcgcttga acgctcttca catgcgactg ccactgaccg cgcaccagcg ccaggtaatc
    23461 tttttgcatc cctttttcac gtaattgctc atgcagagaa cgcaacgccg agcgtttttt
    23521 cgctaccagc aacacacctg aggtgtcccg gtcaagacga tgaaccagtt caaggaaccg
    23581 cgcttccggg cgcaacgccc gcaaaccttc gataacgccg aagcttaaac cactaccgcc
    23641 atgtaccgcc gtaccggaag gtttattcag caccaggata tggtcatctt catataagat
    23701 gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa accgcctctt cttcccgttc
    23761 agcaacgcga accggtggga tacgcacctc atcacccgct tcgagtttat attcaggctt
    23821 aatacgtttt ttgttcaccc gcacttcgcc tttacgcaaa atacggtaaa tcatactttt
    23881 tggtacacct ttcaattggg tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc
    23941 ggtgatagca acaattttta cggatggagt ctctgttttc atggtgggcg attctaatta
    24001 gccaacagga ttcgcgccac tcatttttct atgcttatat ttagttttgc accttattac
    24061 ttcactacat gatcacttta ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa
    24121 actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag gcttgcagtg
    24181 gaataatgag gccgtttccg tgtccatcct tgttaaaaca agaaatttta cggaataacc
    24241 cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat ctttcaggca
    24301 gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg gagagtaata
    24361 cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg caaccgacac
    24421 tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag acacgaggcc
    24481 atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata
    24541 atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg
    24601 ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca gggcacgagc
    24661 agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg
    24721 cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa attgcccgcg
    24781 aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg
    24841 aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa ggcgcggcat
    24901 taaccacctt tatcagtctg gcgggtagct atctggttct gatgccgaac aacccgcgcg
    24961 cgggtggtat ttctcgtcgt atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg
    25021 caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat
    25081 ctgctgaggc gctgcaatgg gatttaagct tccgtctgaa acactgggaa gccatcaaaa
    25141 aagccgctga aagccgcccg gctccgttcc tgattcatca ggagagcaac gtaatcgttc
    25201 gcgcattccg cgattactta cgccaggaca tcggcgaaat ccttatcgat aacccgaaag
    25261 tgctcgaact ggcacgtcag catatcgctg cattaggccg cccggatttc agcagcaaaa
    25321 tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag tcgcagatcg
    25381 agtccgcctt ccagcgtgaa gttcgtctgc catccggtgg ttccattgtt atcgacagca
    25441 ccgaagcgtt aacggccatc gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg
    25501 aagaaaccgc gtttaacacc aacctcgaag ctgccgatga gattgctcgt cagttgcgcc
    25561 tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcca gtacgccacc
    25621 agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgggcg cgtattcaaa
    25681 tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac
    25741 tgggtgaatc cagccatcac gtctgcccgc gctgctccgg taccggtacc gttcgtgaca
    25801 acgaatcgct gtcgctctct attctgcgtc tgatcgaaga agaagcgctg aaagagaaca
    25861 cccaggaagt tcacgccatt gttcctgtgc caattgcttc ttacctgctg aatgaaaaac
    25921 gttctgcggt aaatgcgatt gaaactcgtc aggacggcgt acgctgtgtg attgtgccaa
    25981 acgatcagat ggaaaccccg cactaccatg tgctgcgcgt gcgcaaaggg gaagaaaccc
    26041 caaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag
    26101 aagagttcgc tgaacgtaag cgtccggaac aacctgcgct ggcaaccttt gccatgccgg
    26161 atgtgccgcc agcaccaacg ccagctgaac ctgccgcgcc tgtcgtagcc ccagcaccta
    26221 aatctgcacc ggcaacacca gccgctcctg cccaacctgg gctgttgagc cgcttcttcg
    26281 gcgcactgaa agcgctgttc agcggtggtg aagaaaccaa accgtccgag caaccaacac
    26341 cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc
    26401 gccgtgaccg taatgagcgc cgcgaccctc gtagtgaacg tactgaaggc agcgataatc
    26461 gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca gactgccgag acgcgtgaga
    26521 gccgtcagca ggcagaggta acggaaaaag cgcgtaccac cgacgagcag caaacgccgc
    26581 gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa gaagtgaagg
    26641 cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg
    26701 ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag
    26761 ccgaagaagc ggtagtcgca ccggtggttg aagaaactgt cgcagccgaa ccaattgttc
    26821 aggaagcggc agccccacgc acagaattgg tgaaagttcc gctgccagtc gtagcgcaaa
    26881 ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc
    26941 gtcgttctcg ccgctcgcct cgtcacctgc gcgtaagtgg tcagcgtcgt cgtcgctatc
    27001 gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg
    27061 aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa gatgtacagg
    27121 ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact gaggtccctg
    27181 tcgccgccgc tgccgaacct gttgttagcg cgccagttgt tgaagaagtg gctgaagtcg
    27241 tagaagcccc cgttcaggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg
    27301 aagtgattgc tgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg
    27361 tagcccagga agttgcagaa cacgcagaac cggtggttga accgcaggaa gagacggcag
    27421 atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa gttgttgctc
    27481 aacctgccgc gccagtcgtc gctgaagtca cagcagaagt tgaaacggta gctgcggttg
    27541 aacctgaggt caccgttgag cataaccacg ctaccgcgcc gatgacgcgc gctccggcac
    27601 cggaatatgt tccggaagca ccgcgtcaca gtgactggca gcgccctact tttgccttcg
    27661 aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcatctgcc gctcctgcgc
    27721 gtccgcaacc tgttgagtaa tagttagctc aagtaatcaa gccctggtaa ctgccagggc
    27781 ttttttattt catctttgag tcaacatatt aaaattcatc aaatggcatc cttgctaacc
    27841 aacaatacaa aataggcaaa aaaatgcccg ctccggcgat cccagagcgg gcagacaaaa
    27901 catacccagt ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca
    27961 atccctgcat atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt
    28021 aagatgaaat agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc
    28081 ctaaagcgcg atcgctacct aatgaatcca gcgactccag ttcgttcagt tgcgtgccta
    28141 attccgcgcg aacagtcagc acattgttca gcgagttttt cagtccgcgg ttggttttat
    28201 ccagtgccgc agcggcggtt tctttatccg cttcgctatc cgcgaccggc gttttcaggg
    28261 ctgcgatggc gctatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt
    28321 ccgctaccgc gttgctagta atactgtcga aaattttgtc gcccgtatgt cctatcacca
    28381 tcgaacgcga agcatcgacc tgttgtttaa tactttctgc tccgccttcg tatttgccat
    28441 ccgcctcgct aaacggtgcg gattctgttt tataaccggc aaaaatgtag cgcccgttac
    28501 cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag cccctgaata tccgtagcca
    28561 gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag
    28621 cattctgtat agcggttgtg acctggctaa gtacactctc ttccagtgac actttttgag
    28681 tggcgaaagt acgcgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa
    28741 cagcttgtga tgcagcaatg ggatcatctg aagggttaac gactcgctta cccgtcgaca
    28801 tctgttcgcc gtacttcatc cattctgcct gagaattggt gatgccgcgc atgttttgct
    28861 ggtacatcat ctgggtactg aaacgcattt tatctcccct tagcgaatgt taatcagcgc
    28921 atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc gcgaggtaat actgctgaaa
    28981 acgttgcaga tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt
    29041 ggaaagctgc gtcaccacat taccttgagt ggtgctactg gttttcaacg tcgcggtttt
    29101 attaccgata tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac
    29161 cgttttactg ttgctttgca gatcgagcag ggcctgaccg ttgcggttgt cgctatcacc
    29221 cgcatcttct gcgctcgcca tggcgatttt cgcttcgtcg gtgattaata catccatgtt
    29281 gacgatggcg tcacttaccg gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt
    29341 aaacgtcaac tccagaccat caaatgccac tttaccgttg gcatccggcg tcaccgtaaa
    29401 agtggtattg ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta
    29461 atctgtcgcc agtaccgcgg aggtatcagt taccgttgca ccgatcgcaa cgtcaccttt
    29521 gtttttcgtg ttttgcagaa ccgcgggctt accgatagca aagaaatctt caccggcatc
    29581 gccgttagca tcaaatccgg ctttgtgttg ggtgttgaaa gcctcggcaa atgccagcgc
    29641 cagttgtcca agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc
    29701 gcccagcgac ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc
    29761 aacataagcg acagtcgtac gagaagggtc ggcgctggaa ggaactgccg caagttgccg
    29821 cgccgtactt ccctgaacca gtgagtagcc attggccatc gtgatgttat aagtgccacc
    29881 atcctgaaca ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg
    29941 ttgatccagc agattgttag gtgacgcccc tgcccccacg cctgtcaggc gggagatctg
    30001 atcgttcagg ctggcgattt gtttagcgta attgttgatc tgatcaacac tggcacctat
    30061 cgcgatattg acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt
    30121 caccaaccct tctgatttcc caatcagcgc ctggcgcgct gccggatctt ccgcgttact
    30181 caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact
    30241 ggtggagagc atattgtcga ttttcgacat ctgctcatag cgggcagtca ggccgctact
    30301 ttgcgtctgc gccgcacgta actggttggt aataaacgca tcatactcac gctgcacacc
    30361 agaaacgtag acgccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc
    30421 cataatagtg gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc
    30481 cgtatttaac gccgcctggg ccgcgttcag tccgctcatg gcgttattga tcaagctgga
    30541 catggaggtt ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact
    30601 tgagttattc agaacagatt atcaatgttc atgctgtagg ttttgctcac cttgtcgctt
    30661 atcgatttca tctgctgaat catgttggtg agtttgcggg catagtgagg atcggtggca
    30721 tagcccgcgt cctgtagggc ctgcgccccc tgctccgcac tcgcggcggt cgtcacggcg
    30781 gcgtagcgcg ggttacgcgt taacagccca acgtaatccg acaatgcttc cagatacgag
    30841 ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc cattttcata ttcagtcgtg
    30901 gtgatttcag tgactggccc tttccagttg ccagaggctt tgacaccaaa caggttatag
    30961 ctcggctcgc cgttttcacg gcggatttgc cgttgtcccc agccagattc cagcgccgcc
    31021 tgagcgagga tcaaatgatg tggcacacca ctttgctggc ttgccagttg ggcgggcagc
    31081 gagagttgcg cgaggaatgc tttactgtca cccggcagcg aatcatcgta gttacgtggt
    31141 acggcctttt gcaccagctg cgaaagcgtc tgattttgat aacgcaccac ggtttcgagc
    31201 gggaatttta tcggtgctgc tggcgtggac tcctctggca atggttgttc tggcgtcatc
    31261 tgtttaacca tcatctctgc cagccccaga cctttgcccg ccgtcatctg ttgggcaatc
    31321 tgctggtcat acatactggt atacaggcga gtgtgctcgc tgctgaacag gccatctttt
    31381 ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga acatcccttc cacctggcgg
    31441 gccaccggac ggatatttgc cgctggatct tcgcccgctt tcgcctttag ttcgttgagt
    31501 gattgcgcat cccaggccgc acttgccagt agtttgctgt cgctgatcat cagatgattt
    31561 ccagttttgc ccgcagacat cccgcacttt gcattgattg cagtatggac atcagatcca
    31621 tcggcgtagc gcccagcgca ttgagcgcac gcaccacgtt attgaggctg gcgctggaac
    31681 gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca
    31741 cagtctgccc accaccaaac ggtgtatctg gctggctgac attggcctga cgattaactg
    31801 ttactgagag attcccctgc gctaccgcgc agctgtcgag ggtcacttcg cgattcatca
    31861 ccaccgaacc agtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg acattaacct
    31921 gcatattctg gatatcggca aggaaacgga cctgggaact gttgccactt ggtacgcgca
    31981 cctgaatagt ccgcgcatct aacgcggtgg cgctgccata tccacgcacg cggttgatgg
    32041 tgtcagcgat ttgctgcgcc atactgaaat cttcgtcgtt aagttgcaaa ttaagggtat
    32101 tcccgacgcc aaactggctg ggcaattcac gttcaataac cgcaccattg gtgatccgtc
    32161 caccgttcag ttgattcacc tgaacactgc taccgccagc ggaggcccct gcgccgccaa
    32221 ccagaatatt gccctgcgcc agcgcataca cctgactgtc aacgccctta agcggtgtca
    32281 tcaataacgt accgccacgc agacttttgg catttcccat ggaagaaacc accacgtcaa
    32341 tggtttgccc ctgacgtcca aacggtggaa gtgacgctgt caccataacc gcagcgacgt
    32401 tttttagctg catattagtg cccgtcggaa cggtaattcc cagctgtgag agcatgttat
    32461 taagcgtttg tgtggtaaac ggcgtctgag ttgtctggtc acctgtacca tcaagcccca
    32521 ccaccaggcc atagccaatc agtgagtttt gccttacccc ctgaacactg gtgagatcgc
    32581 gaatacgctc agcctgagcc gccgtggtaa ccagtaaaag aattaatgca gagagaaatt
    32641 taatcaccac agcctcactt acattggcga caggttaagg aagaaacgct gcaaccagcc
    32701 catattttgc gcttcgttaa tgtagccatt gcctacgtat tcaatgcgcg catctgccac
    32761 ctgagtagac ggtacggtat tgctgccgct gatagtgcgt ggattaacca ccccagagaa
    32821 gcgaataaat tcggtaccct gattaatggc gatctgtttt tcacccacca catgcaggtt
    32881 gccgttgacc agtacctggt caaccgtcac cgtcaacgta ccgctaaagg tattgctggc
    32941 attggccccg ccttttccgt tgaacgtgtt accaccggag gcttcgacat cggcacgagc
    33001 gttaccaaac aacccctgca aatagcgcgg cacagtatca aagccaaaat tagttttacc
    33061 gtcacggctg gcattcgcag aggagctttt gctggcgctg acgttctcct gcaacacgat
    33121 agtcagcgta tcgccaatat tgcgtggtcg acgatcttca aacagcggtt gatagccata
    33181 gttaatcggc tgagcagact ggaaaataga cccgttggcg acgggcgtcg gaccgggaac
    33241 cggttgtgca ctggtcgccc cctgtaccag cggcgtggag ggtatccagg cgcagccggt
    33301 tagtgaaagc gccaacaagc tggaaatggc ataagtatgc gcagcgtttt tttgcatggc
    33361 tatcatcttc aaaaatcagt aaaccggtga acctgcccac cggttaagcc ttagagttgc
    33421 gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg
    33481 cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga cgtttcaaca
    33541 tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg
    33601 gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagtccggt gtcattcata
    33661 aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg
    33721 ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct
    33781 ggctgcacct gaaaaccacc ggccgtcacc agctgcccgt tctgatccac ctggaaagag
    33841 ccgtcacggg tataggctga tgagccatct ggcaacatca cctggaaaaa gccctgccct
    33901 ttaatcgcga cgtctttact gttattggtt tgcgacaggt tgccctggct gtgtaaacgt
    33961 tccgtggcga ccgggcgtac ccccgtaccg atttgtaatc cggatggtaa ggtggtttgt
    34021 tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gtagatcttc aaacaccgcg
    34081 cgctgacgct taaaaccgtt ggtactaacg tttgccaggt tgttggcaat gacgtccata
    34141 ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt
    34201 cctgtagtgg attagtgaca aagtgcatat gtcttcttgc cggatgcggc gtaaacgcct
    34261 tatccggcct acaagagctc gcaaaatcga taaattgcga ttgcccccat agacccgata
    34321 aacttgcgca tagggcaact tatacttgtc atgtatcctt tcaattaact catcgacagc
    34381 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    34441 aaacgccgtg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    34501 tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg cccacgcgta
    34561 gcctggcttt ctgcgcttaa acgaaaaatg ccatcgtcac cgcgctggac ttcgctgccc
    34621 gtggctttca ccagcttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga
    34681 ttcagcgccg agattgtgcc atcggcagca atagtgattt ccgccccttc cggcacagca
    34741 attggccccg cctcgcctac caccggatgt ccctgaattg tcagttgccc ggtgggatca
    34801 acctgaatgc tgccattacg cgtataccct tcactgccgt cagcggtctg cacgaccagc
    34861 caaccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc
    34921 gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct
    34981 tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg
    35041 gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgcg
    35101 gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt
    35161 attgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc
    35221 aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca gcgcaccgtt
    35281 ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg
    35341 cgtagcggac cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc
    35401 cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc
    35461 gtcatcattg atttgataac tcaccagatc gcccggtttg tagccgttct gggtggttgc
    35521 cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt
    35581 ggcgggatct gcgccgttaa ttgcgccggt ggtaatatcc tttgttggat ctgaggtcag
    35641 aacgccattg gcattaaaca ccagcgtcgt tgcagggtta gctgtgcctg ttggatcact
    35701 gctatccagg gtgtaaacat cccagttatt atccccggtc ttcacaaagt agacgctcat
    35761 gtcatgagca ttaccctgac tatcgaaaac agtcaccgaa ccttttttgt tatagctatc
    35821 cgcattgctg gcatcaaatg cgttaacaga gggaagcgaa tcactggaat tcaggttgat
    35881 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggaa tcgaaatatt
    35941 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag
    36001 ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt gtccgttacg
    36061 gctgtagaat accgaaccat tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    36121 aacgtccaga ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    36181 agcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    36241 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    36301 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    36361 tgtgaaggct taaattatct gccgtacttc gtcgagggta gtggtgccgt aagtgccaag
    36421 atccagcgta ttaccgttgt tgccgcggat caccccctgc accagagcaa actgcagcgg
    36481 ttgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    36541 cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgga ctccggcagt
    36601 cagttcaccg atatcaatgg tacggacgac cgcgccgttt ttatcggtga tggtggccgt
    36661 cactttgtct gctgcctgtt gcaactcaac accaaacggc gtggtcgtgg tcacagcccc
    36721 ttcttcactg ccggttcccg caagaactgt ggtaccgggg atcatcacgc cgtgaccgat
    36781 aaggttactg gcctgtaacg actgactgtt atcaatctgt ccggaaatag atccgagcgt
    36841 ggtattgagt ttttcaatcc cactgaccgt gctgatttgt gccaattgcg acgtcagctc
    36901 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag
    36961 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    37021 gacgccggta ttcgtcggat cggtggtggt taccgcaata gacatagctt tctcctttat
    37081 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    37141 gcctgataac tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttt
    37201 ggcattttca cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg
    37261 tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggt
    37321 gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc gggaccggtc
    37381 acgctatcag cattcgccag attactggcc gccacgttca gacgctggga ctgggcagtt
    37441 aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt
    37501 aaaacgttca tcatgccttt aatttgcccg ctcaacgcgc taaggctcat ctggtattgc
    37561 aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacagtatt accgtcaagc
    37621 gaaggctggt ccggaatacg gtattgcagt tctgcggaag gaggcgtcag cgcctgcgcc
    37681 ggaatgtgtt gcgttgaggt catcgtcaga gcaaccacac tggttgcatc ccgtccacgt
    37741 tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta
    37801 tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc
    37861 tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca
    37921 aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa
    37981 tgggtcgcta tttatgccgt tgatggtcat tgcggacagg tacaattcac gttgtagaaa
    38041 tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg cgatcatcgc gatactcttc
    38101 agtccgttaa gtacggcgag caatctcaca tcgcaattgc acaacttttt tagcgcccaa
    38161 ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctgctaccg
    38221 ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg caatgtgaat
    38281 gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtaca ggccacagga
    38341 aattatgtgg ttgccgcgat gcccattgtg cgtggaggaa agctggaagc tggcaatgtc
    38401 aaactgaaac gcggacggct ggataccttg ccaccgcgta cggtgctgga tatcaatcaa
    38461 cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccac
    38521 tttcgccagg catggcgggt aaaagcggga caacgcgtca acgttatcgc cagcggtgat
    38581 gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg
    38641 cgggtgcgca tgatatcggg acaagtggtc agcggcgttg ttgatgcaga tgggaatatt
    38701 cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat
    38761 gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag
    38821 ccagtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacaaa cacccgagcg
    38881 gcaaaaacaa cagcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg
    38941 atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa gctggcgatt
    39001 cgtaacggtg aactaaaaat ggacactggc aaaattgccg atgcgctgat caacgaagcg
    39061 cagcaagact tgcagagtaa ttgatcgtat gacgcgtctt gcagagatcc tcgaccagat
    39121 gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat
    39181 ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc
    39241 gacgctggat tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga
    39301 tattagtcaa cgctggcagg aaattgctgt gaaaacgcag caactacgcc aaatgaatca
    39361 acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt
    39421 gaaaccgcat caggaaccga cgctgtatgg ggcgaacggt cagacctcaa ccccctatcg
    39481 cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatgt
    39541 atcgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtact gccagtgcag
    39601 cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaaa cgccagggca
    39661 tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca
    39721 ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc ggagtaaaga
    39781 ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa
    39841 tagaaagtga cagcccggca tgtttcaacg gaccaataaa cgccaggttc atcaattgcg
    39901 ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa
    39961 agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta
    40021 acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa
    40081 cggttaacgg accggaaaga atgcccaacg caaccgcact tggcagcgcc aacaggaaac
    40141 aaagacgcag cccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac
    40201 ttttcgacag cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa
    40261 actccattaa gcggtcggcg taatacatcc acgacaccga accggaagca agaaacgagg
    40321 caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc
    40381 ccatctgttt caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca
    40441 gcatgccgat cttcttcaga tgaggtagct gatacaccag ctgcaggatg ccgccgaccg
    40501 taacagccca ggccagcgcc agcaccggtg gattaaagta cggcgcggca aacagcgcaa
    40561 aaccaatcat gctgatattg agcaatgtcg gggcaaacgc cggaattgag aagcggttcc
    40621 aggtattcag aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg
    40681 ttatctttag tagctggctg gtcagggcaa atttgtcggc agtgtcagcg aagcctggcg
    40741 cggtcaccat gatcacccac ggtgcagcga gcatgccagc gaccgtcaca accgccagcg
    40801 caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct
    40861 tgcttttata ttctgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga
    40921 tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg
    40981 cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg
    41041 tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc
    41101 acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg
    41161 tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg
    41221 tcaacgatac gttgcgccag cacggcctgt tcgccggctg tttgcggaac tgtctgattt
    41281 tgcacgcatt caatgaagtg ccgcgcacag tcgacaaatc cacgttgctc aagcgtactc
    41341 tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg ccattcgcgc
    41401 atatccgtaa tgtcgatcag cgcaccatca gtcacggcct gcacggtttc acgctgactt
    41461 ccggcccggc gatgcataca ggtggtgatc tgcaaaggac cagccgaaaa atggtgctcg
    41521 gcaaacagca tttcgccgga ctcattggtc agcaacgtac cgctttcgag agttgcattg
    41581 ccgccagaca accacagcgc ggtatcaacc acatgcaaat aatcatccag caatgtgaaa
    41641 taaagatcgt gtggcccgac gctattggta cgatgtttat ccattcttag cgaggacgcg
    41701 gtggcgagtt gcgtttttaa ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc
    41761 atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt
    41821 tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg
    41881 ccaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc ggcaaggctg
    41941 gataacgaat cagcataagg aatgcgccag ctgtcacaaa ttggcagggc tttggcgcgc
    42001 gtaggcgacc aggcaccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac
    42061 gctttttgcg cgatgccgcc taaccccact acgccgatac gtaatttttt cactttaatc
    42121 tcccagatgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag
    42181 ggcttcgacg cgggcctgta aatcaccgtc aaccgcgttc gacatatccg ttaccgccgg
    42241 cggattctca acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc
    42301 gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga
    42361 ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc
    42421 cggcgtctgg gcaccacgca ataacagcgt ggtgattaac gccacttctg cggcgctcag
    42481 tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc
    42541 aaaaccgctc accgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac
    42601 ttcagattca ctcagattca taaccggttc acggttcgtt ttctgattac aggccgtgac
    42661 tacaccattt acggagagcg ggtattgctc cggcgtggtc acctgttttt ccagcaagca
    42721 accaatcacg cgggcttcca gggcggtcag ttgatatttc atgagattct ccttagcggc
    42781 cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa
    42841 tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac
    42901 cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct
    42961 gcatataacg aatggctgcg ctcagagctt caaacatgag tcctttgccc tgccattttt
    43021 gcccaatcga ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat
    43081 tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac
    43141 cttgtttatg aaattcgtta atcatcccta gcctggcctg ccagcctgat ggataacagt
    43201 ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat
    43261 ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta
    43321 agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa
    43381 cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca
    43441 gaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt
    43501 tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga
    43561 aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc
    43621 tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct
    43681 tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta
    43741 ttgccctcgg tctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg
    43801 ccgaccgctt tggtgccaaa ccgatgattg tcaccggtat gctgatgcgt gccgccggat
    43861 tcgccacaat gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc ctgctttcgg
    43921 gactcggcgg cacgttgttt gatcctccgc gttcggcgct ggtggtgaaa ctaatccgtc
    43981 cacaacagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca
    44041 ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag
    44101 gggcagtttt gtttgtgctg tgtgcggcgt tcaatgcgtg gttgttacca gcatggaaac
    44161 tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt
    44221 ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga
    44281 tgctgccaat tatggtcaac gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg
    44341 ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc
    44401 attttcgtct ggaacaccgg ttaatggctg ggctgttgat aatgtcatta agcatgatgc
    44461 cagtgggcat ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg
    44521 ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggca gacgcaagag
    44581 ctcgcggcag ctatatggga tttagccgtc tgggtctggc gattggcggc gctattggtt
    44641 atatcggtgg cggctggctg tttgacctgg gcaaatcggc gcaccagcca gagcttccgt
    44701 ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc
    44761 agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac
    44821 tcctccatac tgaaagagaa ctgacagcca aatggaggag tcacgtgaag ctatacattt
    44881 acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg
    44941 tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa
    45001 aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc atggacatcg
    45061 ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca
    45121 ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg
    45181 ccaaatcggc atttgatgag ttttctactc ctgccgcgcg caaatatttc gtcgacaaga
    45241 aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga
    45301 atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg
    45361 gcgaactttc ggaagatgat attcagctat ttccgctcct gcgtaatctg acgctagtag
    45421 ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac
    45481 aaatcaattt gttatcatca atggcgattt aatcctgacc cgtcgcggca gcctgtattc
    45541 gcgggttgcc gccgttttca gttttttctg ctggcgcgtt tatcgctggt gacacatgct
    45601 gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta
    45661 tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac
    45721 accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat
    45781 atcggtttac ccggcgtggc tgacgcccat atcgttctga caaacctgac cagccaaatt
    45841 ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga catgaactcc
    45901 ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat
    45961 aaagaaaaag gcgcgatctt cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg
    46021 gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt taaaccaggc attacgcaat
    46081 tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg
    46141 gcgaagaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccgtttact
    46201 gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc
    46261 ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca
    46321 ccatcccagg ttttaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc
    46381 gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg
    46441 cccaatctgg ctccgcccgt gaccaccgta gaggctgccg tggcgtatcg ccagcgtatt
    46501 cttgacgccg tacctgccgg gcacgatttc accccgctga tgacctgtta tttaacagat
    46561 tcgctggatc ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa
    46621 ctttatccgg caaatgcaac cactaactcc agccacggcg ttacgtcagt tgacgcaatc
    46681 atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tac
//
LOCUS       DAJOTB010000019        43839 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJOTB010000019 DAJOTB010000000
VERSION     DAJOTB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.019"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(127..3276)
                     /locus_tag="OHN28_001080"
     CDS             complement(127..3276)
                     /locus_tag="OHN28_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCQ2296516.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="OHN28_001081"
     CDS             complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="OHN28_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCQ2296517.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4634..5281
                     /gene="acrR"
                     /locus_tag="OHN28_001082"
     CDS             4634..5281
                     /gene="acrR"
                     /locus_tag="OHN28_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCQ2296518.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5409..8771
                     /gene="mscK"
                     /locus_tag="OHN28_001083"
     CDS             5409..8771
                     /gene="mscK"
                     /locus_tag="OHN28_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCQ2296519.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8810..8974)
                     /gene="rsmS"
                     /locus_tag="OHN28_001084"
     CDS             complement(8810..8974)
                     /gene="rsmS"
                     /locus_tag="OHN28_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCQ2296520.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(8988..9515)
                     /gene="priC"
                     /locus_tag="OHN28_001085"
     CDS             complement(8988..9515)
                     /gene="priC"
                     /locus_tag="OHN28_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCQ2296521.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9585..9962
                     /locus_tag="OHN28_001086"
     CDS             9585..9962
                     /locus_tag="OHN28_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCQ2296522.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10115..10666
                     /gene="apt"
                     /locus_tag="OHN28_001087"
     CDS             10115..10666
                     /gene="apt"
                     /locus_tag="OHN28_001087"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCQ2296523.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10795..12726
                     /gene="dnaX"
                     /locus_tag="OHN28_001088"
     CDS             10795..12726
                     /gene="dnaX"
                     /locus_tag="OHN28_001088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCQ2296524.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12059..12123
                     /gene="dnaX"
                     /locus_tag="OHN28_001088"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12779..13108
                     /locus_tag="OHN28_001089"
     CDS             12779..13108
                     /locus_tag="OHN28_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCQ2296525.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13108..13713
                     /gene="recR"
                     /locus_tag="OHN28_001090"
     CDS             13108..13713
                     /gene="recR"
                     /locus_tag="OHN28_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCQ2296526.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13823..15697
                     /gene="htpG"
                     /locus_tag="OHN28_001091"
     CDS             13823..15697
                     /gene="htpG"
                     /locus_tag="OHN28_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCQ2296527.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15878..16522
                     /gene="adk"
                     /locus_tag="OHN28_001092"
     CDS             15878..16522
                     /gene="adk"
                     /locus_tag="OHN28_001092"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCQ2296528.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16654..17616
                     /gene="hemH"
                     /locus_tag="OHN28_001093"
     CDS             16654..17616
                     /gene="hemH"
                     /locus_tag="OHN28_001093"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCQ2296529.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17613..18572)
                     /gene="aes"
                     /locus_tag="OHN28_001094"
     CDS             complement(17613..18572)
                     /gene="aes"
                     /locus_tag="OHN28_001094"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCQ2296530.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18724..20028
                     /gene="gsk"
                     /locus_tag="OHN28_001095"
     CDS             18724..20028
                     /gene="gsk"
                     /locus_tag="OHN28_001095"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCQ2296531.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20158..21834)
                     /locus_tag="OHN28_001096"
     CDS             complement(20158..21834)
                     /locus_tag="OHN28_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCQ2296532.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22073..23293)
                     /gene="fsr"
                     /locus_tag="OHN28_001097"
     CDS             complement(22073..23293)
                     /gene="fsr"
                     /locus_tag="OHN28_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCQ2296533.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23511..25163
                     /gene="ushA"
                     /locus_tag="OHN28_001098"
     CDS             23511..25163
                     /gene="ushA"
                     /locus_tag="OHN28_001098"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCQ2296534.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25201..25704)
                     /locus_tag="OHN28_001099"
     CDS             complement(25201..25704)
                     /locus_tag="OHN28_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296535.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25701..26501)
                     /locus_tag="OHN28_001100"
     CDS             complement(25701..26501)
                     /locus_tag="OHN28_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296536.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26525..27004)
                     /gene="ybaK"
                     /locus_tag="OHN28_001101"
     CDS             complement(26525..27004)
                     /gene="ybaK"
                     /locus_tag="OHN28_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCQ2296537.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(27208..28002)
                     /locus_tag="OHN28_001102"
     CDS             complement(27208..28002)
                     /locus_tag="OHN28_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCQ2296538.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            28135..28437
                     /locus_tag="OHN28_001103"
     CDS             28135..28437
                     /locus_tag="OHN28_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2296539.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            28473..28814
                     /locus_tag="OHN28_001104"
     CDS             28473..28814
                     /locus_tag="OHN28_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2296540.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(28872..31376)
                     /gene="copA"
                     /locus_tag="OHN28_001105"
     CDS             complement(28872..31376)
                     /gene="copA"
                     /locus_tag="OHN28_001105"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCQ2296541.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            31638..32570
                     /gene="glsA"
                     /locus_tag="OHN28_001106"
     CDS             31638..32570
                     /gene="glsA"
                     /locus_tag="OHN28_001106"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCQ2296542.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            32573..33865
                     /locus_tag="OHN28_001107"
     CDS             32573..33865
                     /locus_tag="OHN28_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2296543.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            33990..34397
                     /gene="cueR"
                     /locus_tag="OHN28_001108"
     CDS             33990..34397
                     /gene="cueR"
                     /locus_tag="OHN28_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCQ2296544.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(34398..35133)
                     /locus_tag="OHN28_001109"
                     /pseudo
     CDS             complement(34398..35133)
                     /locus_tag="OHN28_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35227..35685)
                     /locus_tag="OHN28_001110"
     CDS             complement(35227..35685)
                     /locus_tag="OHN28_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCQ2296545.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(35682..36599)
                     /locus_tag="OHN28_001111"
     CDS             complement(35682..36599)
                     /locus_tag="OHN28_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCQ2296546.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36745..37422
                     /gene="fetA"
                     /locus_tag="OHN28_001112"
     CDS             36745..37422
                     /gene="fetA"
                     /locus_tag="OHN28_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCQ2296547.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            37409..38188
                     /gene="fetB"
                     /locus_tag="OHN28_001113"
     CDS             37409..38188
                     /gene="fetB"
                     /locus_tag="OHN28_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCQ2296548.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(38371..39225)
                     /gene="cnoX"
                     /locus_tag="OHN28_001114"
     CDS             complement(38371..39225)
                     /gene="cnoX"
                     /locus_tag="OHN28_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCQ2296549.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(39286..40095)
                     /gene="ybbO"
                     /locus_tag="OHN28_001115"
     CDS             complement(39286..40095)
                     /gene="ybbO"
                     /locus_tag="OHN28_001115"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2296550.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAIMVLKRLLPGRVMDKILQG"
     gene            complement(40085..40708)
                     /gene="tesA"
                     /locus_tag="OHN28_001116"
     CDS             complement(40085..40708)
                     /gene="tesA"
                     /locus_tag="OHN28_001116"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCQ2296551.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            40679..41365
                     /locus_tag="OHN28_001117"
     CDS             40679..41365
                     /locus_tag="OHN28_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2296552.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            41362..43776
                     /locus_tag="OHN28_001118"
     CDS             41362..43776
                     /locus_tag="OHN28_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2296553.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT        10530 a        11459 c        11595 g        10255 t
ORIGIN      
        1 aattttatac gtggttaata ctggttttcg tatgggatcc tgagttggtg gttcaataca
       61 tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg cgcggcctta gtgattacac
      121 gttgtatcaa tgatgatcga cagtatggtt gtgctcgata tcttcattct tgcggctaaa
      181 gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta ccgttgcggt
      241 caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac cagcaccagt
      301 actgataacc agcggcataa cgccgaggat aaacgccaac gaggtcatca ggattgggcg
      361 taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt ctttatccat
      421 caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaaac caatggttgt
      481 gagcaggcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa
      541 cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg accagctctc
      601 gtacagcgca gccagacaca ggaacacgac aatcaacgaa atcgcgtaca gtgaaggtgc
      661 ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc caacaccggt
      721 aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac ttttacccgg
      781 tgccgcctgg cctaagattt ccatagatgg caggccgttg taacgttcca gacgcggcga
      841 accgtactcc caacgagaag aggagaacgc cgagaacggc accatctgac catcagcagc
      901 acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgcct ctgacatgac
      961 gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac cgccccatgc
     1021 agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg
     1081 gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta cgctggtcaa
     1141 catatcaggg tgcttcgctg cttctgcaag caactggtta cgcgcctgag tcagtttttc
     1201 gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcgg tacccagttc
     1261 cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg agaaagcacg
     1321 tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat cggcccagtc
     1381 cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt
     1441 aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat tcgttacctc
     1501 attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat
     1561 aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacga acagataggc
     1621 catgccgacc acgatgatca gatacagcac caggtaacgc cccgtactgc gtagaatacc
     1681 gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa accagccgaa
     1741 gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca
     1801 aagagccgga gtcaggatca acgccaccag taccgacagc gccattgctg aaacaatggt
     1861 aatagagaac tgacgataga tagcaccagt agaaccgcca aagaaggcca tcggtacgaa
     1921 taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc ccatcgactt
     1981 acgggtggct tcttttggtg gcaaaccttc ttccgccatc acacgctcaa cgttttctac
     2041 cacaacgatg gcgtcatcca ccaacaggcc gatggcgagc accatcccga acattgttag
     2101 cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca ataccaccgg
     2161 tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aagaacagat acattaccag
     2221 gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag agattttcac
     2281 gaacggtgtg gtgtcatacg ggtaaacaat tttcagaccc gacgggaaga acggttccat
     2341 cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgttcgcac cggtcgccag
     2401 cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt cgtagttctc
     2461 accacccagc tcaattttcg ccacatcacg cagcagcacg cgggaaccat cctgattcac
     2521 tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag caataataga
     2581 ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgaccag ctgcaacctg
     2641 ggcgttctgt gctttgatgg cggtaatgac atcaaccggc gttagctgga atttgttcag
     2701 ttcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact gaacgtcacc
     2761 cacgcccgac gtacggctga tggcatcttt catattcgcc gccacgtagt cggagatatc
     2821 ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga agctgctgga
     2881 tgatttctca acgctcaccc cttgctgctg tacttcttgc ggcagcaacg gcatcgccag
     2941 ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag actcaaaggt
     3001 cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca tcaggttatc
     3061 gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc
     3121 agcgccaggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag gatattgcgc
     3181 caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg cgatcaccca
     3241 cgcaaaaatt gggcgatcga taaagaaatt aggcatgtct taacggctcc tgtttaagtt
     3301 aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa
     3361 cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt attactacgc
     3421 gatcgcctgc tttcagacct tctgtcacca gccacttatc gccgatagcc tggcttgcaa
     3481 cgatcggacg ggtttccact ttgtcatccg cgccaaccac cagtacggtg gcatcgccac
     3541 gtggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta agcccttctt
     3601 ccagacgtgc acgcacgaac atacccggca gcagagtgtg atccgggttc gggaagatag
     3661 cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat tccagcgtac
     3721 cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct ttgccgttct
     3781 cttgtttcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg ttgctggact
     3841 gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac
     3901 cgttctgtac caatgcgcct tccgtcacgt ttgacttacc aatgcgacca ctaatcggag
     3961 aggtgacttt ggtgtaagcc agattgattc gcgcagtttc aacggcagct ttcgccgcag
     4021 ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct tgtttactga
     4081 tgtactgagt accgagcaat ttctgataac gattcaccgt caattgcgcg atattggctg
     4141 cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgccgcctga taggtcgcag
     4201 gatcaatctg atagagagag acacctgctt cgatgtcgct accttctttg aaattacgct
     4261 tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac
     4321 ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg ccaacggcgg
     4381 gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc
     4441 ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc atatgtaaac
     4501 ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt
     4561 tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct aacgcctgta
     4621 aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc gccaacacat
     4681 cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct cgctgggtga
     4741 gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta aagacaagtc
     4801 ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac tcgagcttga
     4861 gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat taattcatgt
     4921 tcttgagtcc acggtgacag aagaacggcg tcgattattg atggagatta tattccacaa
     4981 atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc tctgtctgga
     5041 aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc
     5101 ggatttaatg acgcgtcgcg cagcaattat tatgcgcggc tatatttccg gcctgatgga
     5161 aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc
     5221 catcttactg gagatgtatc tcctgtgccc cacgcttcgt aaccctgcca ctaacgaata
     5281 accctgaatc tgactccagg aattttcctg gacattttcg tcgttgctat tctggttcac
     5341 tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt
     5401 cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt ttttcagcat
     5461 ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcacgg gcgtcatcga
     5521 atggtgattt accgacaaaa gcggacctgc aggcacaact tgactcactg aataaacaaa
     5581 aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca ttagccaccc
     5641 tcgataaaat cgatcgcgta aaagaagaga cagttcagct acggcaaaaa gtcgctgaag
     5701 cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagtgat gtcgataacg
     5761 acgaagaaac gcgcaaaatt ctgagcgcgc tttcgttgcg ccagctggaa actcgcgttg
     5821 cccaggcgct ggacgatttg caaaacgcac aaaatgatct ggcgtcttat aacagccaac
     5881 tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc
     5941 agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact gccttacgtc
     6001 ccagccagaa agtgttaatg caggtccagc aggctttgct gaatgccgag attgaccagc
     6061 agcgtaaaag cctggaaggg aacactgtct tgcaggatac cttgcaaaag cagcgtgatt
     6121 acgtgacggc gaacagcgcc cgtctggagc accagttaca actgttgcaa gaagcggtaa
     6181 acagcaagcg cctgacttta accgaaaaaa cggcgcagga agctgtctcc ccggatgaag
     6241 ccgcgcgtat tcaggctaat ccgctggtga agcaggaact ggaaattaac cagcagttaa
     6301 gtcagcgtct gattaccgcg actcaaaacg gtaatcagtt gatgcagcaa aacattaaag
     6361 tcaaaaactg gctggagcgt gcgctgcaat cggaacgtaa tattaaggag cagattgccg
     6421 ttctgaaggg cagtctgctg ttgtcacgta tcctgtacca gcaacaacaa acactcccct
     6481 cggcagatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg
     6541 aagtcaacca gcagcgtgac gcgctgttcc agagcgatgc gttcgtcagc aaactggaag
     6601 aaggtcacac caacgaagtc aacagcgaag ttcacgatgc attattgcaa gtagttgata
     6661 tgcgtcgcga attgctggat cagcttaaca aacagttggg taatcagctg atgatggcca
     6721 ttaacctgca aattaaccag cagcagttaa tgagcgtatc gaaaaatctg aaatccatcc
     6781 tgacgcagca aattttttgg gtgaacagta accgcccgat ggactgggac tggattaaag
     6841 cgttcccgca aacgctgaaa gatgaattta agtcgatgaa aatcacggtg aactgggaaa
     6901 aagcctggcc cgccgtattc atcgccttcc tcgctggttt gccgctgctg ttgattgccg
     6961 ggctgatcca ctggcgtctg ggctggctga aagcgtatca acaaaaactg gcttccgctg
     7021 tgggttccct gcgtaacgac agccagctca acacaccaaa agcgatcctt atcgacctga
     7081 tccgcgcact gccggtgtgc ctgattattc tcgctgtcgg cctgattctg ctgactatgc
     7141 agctcaatat cagcgagtta ttgtggtcgt tcagcaaaaa actggcgatt ttctggctgg
     7201 tgtttggcct gtgctggaaa gtgctggaga aaaatggtgt tgccgtgcgt cactttggta
     7261 tgccggaaca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagc ctcgcgttgc
     7321 tgccgatcca tttctggtct gtggtggcag aactctcccc gctgcatctg atggatgatg
     7381 tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg gtgtggccga
     7441 tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc accattaccg
     7501 tgctgtcgat tatcccgatt gcgttgatgg tgctgaccgc cacgggttac ttctacacca
     7561 cgctgcgcct ctccgggcgt tggattgaaa ccgtttattt ggtgatcatc tggaacctgc
     7621 tgtaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc tggcgccgtg
     7681 cactggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag
     7741 aacccaccat tgcgctggag caggttaacc agcagacgct gcgtattacc atgttgctga
     7801 tgtttgcgct gttcggtgtc atgttctggg caatttggtc tgatttgatc accgtgttca
     7861 gctatctcga cagcattacg ctctggcatt acaacgctac tgaagctggt gctgcggtgg
     7921 tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg gtggcctggg
     7981 cgttgattcg caacctgcct ggtttgctgg aagtgctggt gctctcgcga ctgaatatgc
     8041 gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt gccgttggtg
     8101 cgatgacggt gttcggatcg ctgggcgtct cgtgggataa actccagtgg ctggcggcag
     8161 cattatccgt aggtcttggt tttggtttac aagagatctt cggtaacttc gtttccggtt
     8221 tgatcattct gttcgaacgt ccggtgcgca ttggcgacac ggtaaccatt ggtagcttct
     8281 cgggtacggt cagcaagatc cgtattcgtg cgacaacgat taccgatttc gatcgcaaag
     8341 aagtgattat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg
     8401 acacgactac gcgtctggtg atccgtctcg gcgtagccta tggctccgat ctggaaaaag
     8461 tgcgtaaagt gttgctgaag gcggcgactg agcacccaag ggtgatgcac gaaccaatgc
     8521 cggaagtctt ctttacggca tttggtgcca gcacgttgga tcatgagctg cgtctgtatg
     8581 tgcgtgaact gcgtgaccgt agccgtactg tcgatgagct gaaccgtact attgatcagt
     8641 tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat ctgcacaacg
     8701 agaagggcga tgaagtgacg gaagtgaaac gcgactacaa aggcgacgat ccgacgccag
     8761 cggtagggta aggacaataa agggcgacag tgatgccgcc ctttattttt tactcgcttt
     8821 gtgaagtctc atcgctctgt aacttctttt cataatcgcg ttttacaata tccagcgcgc
     8881 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacggcca
     8941 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaaa
     9001 cgcgctaacc tgttttcgat tttttccagc gcatggcggc aacgtgccag gcgcgcttca
     9061 taggcttcca cttcacgatg cagcgtctgc tgttccacga gatcggtcgc tcgcgccaga
     9121 cgggctctac gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
     9181 cgtttacgct gccagcgggc aattttcggt ggtgcgctat cccactcgcg tagcgaccag
     9241 gctgtggctt cacgcgctat agcctccagt tgcgccgcca gatgttctgc cagccaggcg
     9301 acttgcggca gctgttgctg ctcaaccgca tgacgaagcg cagccaggtt atcgcccgac
     9361 tcatcgagac aagcctgtag tgttgtcgca cgggtctgaa aaaggtgcct gtcaaaacgg
     9421 gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt ggcgagctgt
     9481 ccttccagtt tttccagtag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
     9541 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
     9601 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
     9661 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
     9721 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
     9781 atcatgcgat gccgcgcggc gcaaaaccac gggcgatttt gcttattttg attacgttta
     9841 cgatttcgct gtggttcgtc cagatgccgt gggtgcgcat catgttgctg gtaattctcg
     9901 cctgtttgct tttttatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
     9961 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    10021 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt caaaacgcgc
    10081 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    10141 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    10201 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    10261 tgagcgttac aaaaatgcgg gcattaccaa agtggtcggc accgaagcgc gtggcttctt
    10321 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    10381 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcaact
    10441 ggaaatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    10501 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    10561 agctgacgct gcgttcatta tcaacctgtt cgatctcggc ggtgaacagc gtctcgaaaa
    10621 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgtcagtctt
    10681 gcgctgctca cgctacgggc agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    10741 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
    10801 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    10861 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    10921 ttttccggca cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    10981 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    11041 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgtac caaagttgaa
    11101 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    11161 tatctcatcg acgaagtgca tatgctgtcg cgtcacagct ttaacgcact gttaaaaacc
    11221 cttgaagagc cgccagagca cgttaagttt ttgctggcga cgaccgatcc gcagaaattg
    11281 ccggtgacga ttttgtcacg ctgtctgcaa tttcatctca aggcgctgga tgtcgagcaa
    11341 attcgccatc agcttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
    11401 ctgcaattgt tggcacgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
    11461 caggcgattg ccagcggtga cggccaggtt tcaacccagg gggtcagtgc gatgctgggt
    11521 acgcttgacg acgatcaggc gttatcgctg gttgaagcga tggtcgaggc caacggcgag
    11581 cgcgtaatgg cgctaattaa tgaagctgct gcccgtggta tcgagtggga agcgttgctg
    11641 gtggaaatgc tcggtctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    11701 ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
    11761 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
    11821 ccggaccgcc gtatgggcgt tgagatgacg ctgctgcgcg cactggcgtt ccatccgcgt
    11881 atgcctctgc ctgatccaga agtgccacgc cagtcctttg cgcccgtcgc gccaacggca
    11941 gtaatgacgc caacacaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
    12001 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
    12061 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
    12121 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgcccggtg
    12181 ccatcggcgc tggaaaaagc gccagccaaa aaagaagctt atcgctggaa ggcgaccact
    12241 ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
    12301 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacccg
    12361 tgggcggctc aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat
    12421 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
    12481 ttgaacaacc gcggcgcaca gcaaaaactg gctgaagcgt tgagtacgtt caaaggttca
    12541 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggaatgg
    12601 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
    12661 aatattcaga ccctgcgtcg attcttcgat gcggagctgg atgaagaaag tatccgcccc
    12721 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
    12781 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
    12841 gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct
    12901 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
    12961 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
    13021 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
    13081 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
    13141 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
    13201 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
    13261 tcggaaatcg gccactgcgc cgattgtcgc accttcaccg aacaggaagt ctgtaacatc
    13321 tgttcgaatc cacgtcgtca ggaaaacggt caaatctgcg tggtggagag tccggcggac
    13381 atttacgcca tcgagcagac ggggcagttt tcaggtcgtt attttgtgtt gatgggacac
    13441 ctgtcaccgc tggacggcat cggtcctgat gatatcgggc ttgatcgtct ggaacagcgt
    13501 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca accccacggt tgaaggtgaa
    13561 gctaccgcta actacattgc cgagctttgt gcgcaatatg acgtggaagc cagccgaatc
    13621 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
    13681 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
    13741 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
    13801 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
    13861 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
    13921 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgcg cgctttctaa
    13981 cccggacctg tacgaaggtg atggtgaact acgcgttcgt gtctctttcg ataaagacaa
    14041 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
    14101 tctggggact atcgctaaat ccggcaccaa atcattcctc gaatccctgg gttctgacca
    14161 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
    14221 ggctgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
    14281 ctgggaatcg gctggcgaag gtgaatacac cgttgccgac atcactaaag aagatcgtgg
    14341 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgtgt
    14401 gcgttccatc atcagcaaat attccgacca tatcgcgctg ccggtagaga tcgaaaaacg
    14461 cgaagagaaa gacggcgaaa ccattatctc ctgggagaaa atcaacaaag cgcaggcgct
    14521 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
    14581 cgcccacgac tttaacgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
    14641 gtacaccagc ctgctgtaca ttccgtctca ggcaccgtgg gatatgtgga accgcgatca
    14701 taaacacggt ctgaaactgt atgttcagcg tgtgttcatc atggacgacg cagaacagtt
    14761 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
    14821 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
    14881 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
    14941 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca
    15001 ggaagcgatc gccaaactgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
    15061 cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactatat
    15121 caccgcagac agctacgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
    15181 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
    15241 tgagttcgac ggtaaaccgt tccagtctgt gtctaaagtt gacgagtcgc tggaaaaact
    15301 ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga
    15361 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
    15421 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact
    15481 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
    15541 tcacgtactg gtgaaacgtg cggcggatac tgaagatgaa gcgaagttca gcgagtgggt
    15601 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
    15661 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gaccttcgtg
    15721 tcatccggca tttttctttt cttcatctgc actttccgca aattatcttg ccattaaccg
    15781 tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt
    15841 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
    15901 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
    15961 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
    16021 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
    16081 taaagagcgc attgctcagg aagactgccg caacggtttc ctgttggacg gcttcccgcg
    16141 taccattccg caggcagacg caatgaaaga agcgggcatc aatgttgatt acgttctgga
    16201 attcgacgta ccggacgaac tgattgttga ccgtatcgta ggccgccgcg ttcacgcgcc
    16261 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt
    16321 taccggtgaa gagctgacta cccgtaaaga cgatcaggaa gagaccgtac gtaaacgtct
    16381 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
    16441 gggtaacacc aaatacgcga aagttgacgg caccaagccg gtggctgaag ttcgcgctga
    16501 tctggaaaaa atcctcggct aatcaagccg cagcagatct gctttcgggc aggtctgctc
    16561 atcctcctca ggcaatacct cttacagcaa ttagttcttc ttcttcactt ttccgctaca
    16621 attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg
    16681 ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa
    16741 caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg gccgttgctg
    16801 cgcggcgtga ttttgccgct gcgttcgccg cgtgtggcga agctgtatgc ctctgtctgg
    16861 atggaaggtg gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcgcaa
    16921 cgtttaccgg agacgcccgt ggcgctggga atgagctacg gctcgccatc actggaaagc
    16981 gccgtagatg aacttctggc agagcatgta gatcatattg tggtgctgcc gctttatccg
    17041 caatactcct gttcaacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc
    17101 aaacgtagca ttccggggat atcgtttatt cgtgattacg ccgataacca cgattatatt
    17161 aatgcactgg cgaacagcgt acgtgcttct tttgccaaac atggcgaacc ggatctgctg
    17221 ttgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa
    17281 cgttgccgca caacaactcg cgaactcgct tccgcactgg agatggcacc ggaaaaagtg
    17341 atgatgacct ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta taccgacgaa
    17401 acgctgaaaa tgctcggaga aaaaggcgtg ggtcatattc aggtgatgtg cccgggcttt
    17461 gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga agtcttcctc
    17521 ggcgctggcg ggaaaaaata cgaatatatt ccggcgctta atgctacacc ggagcatatc
    17581 gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg
    17641 ccgtcgcgaa gagcctcgtc ggcggttttc atcatccgtg aataatgcaa aaaggcgtgc
    17701 agcgtgcctg ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa
    17761 agcagatggc tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcgga
    17821 acttcgcgag tgagatcatt attaaacagg cagtaatatg gcgactcgcg gtccgcgtcg
    17881 ttgcttaaat acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca
    17941 ccacccaaca gacgacgagc cacggaatcc cgtaatccgt aaagcccata ccacagcaaa
    18001 acgcccgcaa ctttaccgca atcgatctgt ttatcgcgca accacaacgc actggcgagc
    18061 gccagcatgg cacctgcgga atcaccggca aaaccaatgc gggacatatt gatttgataa
    18121 tcctccgcct gctggtggaa ataacaacag gcagccacaa tttcctctat cgcttgcgga
    18181 aaacgcgctt caggtgaaag ggtgtaatca atgccaatca ccgtacattg gctgtagctt
    18241 gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca
    18301 tgcaaataaa atagcgtcgc cgggctatct ggctgcggat aaaagatacg tgttttcacc
    18361 tgcccatatt ttgttggaac cctgtaagct ctggttgcca tttctggagc gtccgcattc
    18421 cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgctcc cgttgcaggc
    18481 cagggcggta aatccggctg aagagtattc acaacggtct tcatttcagc agagataagg
    18541 tccagaacgg gtaatttgtt ttccggcttc atgcaaaaac tcctttcaaa ttacgtcatt
    18601 gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc
    18661 tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca
    18721 accatgaaat ttcccggtaa acgtaaatcc aaacattact tccccgtaaa cgcacgcgat
    18781 ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ctgcctgggt agtgggtatc
    18841 gatcaaacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga
    18901 ttaagcgccg ggcattcact ggtgattgag gatgacgttg ccgaagcgct ttatcaggaa
    18961 ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg
    19021 cacaactact cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat
    19081 attgaaattg gcagctatgc ctatcgttac ctgtgtaaca cctccagccg taccgatctt
    19141 aactatctac aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc
    19201 ggggaacgta cctttgctat cagcccgggc cacatgaacc agctgcgggc tgaaagtatt
    19261 ccggaagatg tgattgccgg agcctcggca ctggttctca cttcttatct ggtgcgttgc
    19321 aagccgggtg aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat
    19381 aacgtaccgg tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg
    19441 tggcagcaat tcctcaaaga ccacgtctct atccttgcga tgaacgaaga tgaagccgaa
    19501 gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat
    19561 ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa
    19621 gcgaaacgta aaacccagca tccgctgctg ccgggggcta tagcggaatt taatcagtat
    19681 gagtttagcc gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac
    19741 attgcgccgt acatgggcgg gccggaaaaa atcatgaaca ctaatggagc aggggatggc
    19801 gcactggcag cgttgctgca tgacattacc gccaacagct accaccgtag caacgtacca
    19861 aactccagca aacataaatt cacctggttg acgtattcgt cattagcgca ggtgtgtaaa
    19921 tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc
    19981 ttgccggagc gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt
    20041 tcgtaggcca gataaggcgt tcacgccgca tctggcattt tgctctcaat gcctgatgcg
    20101 acgctggcgc gtcttatcag gcctacatat ccgcgcctat tatccggcaa ccaccgttta
    20161 cctcgtcacc acctcacccg ccggtggcgt ttccagcagt tccagcatag tacgggcaat
    20221 ttcacgctca cccatcacta cctgattcgc accacgttcg gtgatatacg ccacttcatc
    20281 gtcataatgg gcgcgggcaa taatctcaat atccggattt ttcgcgcggg cagatgccac
    20341 aatctcgccc gcttcatagc cgttgggaat cgtcaggatc agccattttg cacattccag
    20401 atgcgccagt tgcataattt cttcgttagc cgcattgccc aatactgcgc ggaccccgcg
    20461 ctcgcgcagc tcatcaacac gggttcgtga cgtctcaatc accaccagcg gaatatcaga
    20521 ggcgagcaat ttctccccca gcaggctgcc tacacgaccg taacccacca gtagcgcatg
    20581 gttgcaaata tccactggga tctgcttctc ttcttcgatt gcctcttcca gcgtctgctc
    20641 ttccagcgtt tcggtcttcg ccagatattt ctccagtagt gcgaacagta ccgggttgag
    20701 cataatcgac aggatcgccc ctgccagtac caggttttgt ccggcctgcg gcagtaaatt
    20761 caatgccatt cctagtcccg ccaggataaa cgcgaactca ccaatctgcg ccaggctggc
    20821 ggcgatggtt aacgccgtgc gctgggagtg accaaacaaa cgcacaagga acagggccgc
    20881 gaccgattta ccaaacagaa taattgccag cgtcgccagt actgccagcg gttgctgaat
    20941 cagaattaac ggatcaaaca acatcccgac ggagacaaaa aacagcaccg caaacgcgtc
    21001 gcgcaatggc agtgtatcgt gggcggcacg gtgactcagt tcagactcgt tcagtaccat
    21061 cccggcaaag aacgcaccga gtgcaaagga gacatcaaac agctctaccg caccaaaggc
    21121 aatccctaac gccagcgcca gcaccgacag ggtaaacagc tcgcgggaac cggttgctgc
    21181 gctgcgtgcc ataatccacg gcaccagacg gcgacctacc agcatcataa tggcgataaa
    21241 tgcgatcacc ttgccgatgg tgatccccat atcgactgca agagtggcaa agcccacatc
    21301 gccctgttcc atcattcctg ctaccgcggg tagcaatacc agtgtcagga ccatcaccag
    21361 gtcttccaca atcaaccaac cgatggcgat ttgcccacgc tgactgtcaa ttaattgccg
    21421 ttcttcaagt gcgcgcagta acaccacggt actggcggtg gaaagacata agccgaacac
    21481 gataccggtc attaacgacc agcccagcac ggcggagagc gccataccca gcagcgtcgc
    21541 cacggctatc tgggcgatcg cgccgggaat ggcgatggac tttaccgcca tcaaatcctt
    21601 gagcgaaaaa tgcaaaccga cgccgaacat cagcaaaata acgcccagtt cagccagttc
    21661 cggcgcaagc ttagtatcgg caacaaagcc cggagtgaat ggtcctgcca gcacacccgc
    21721 taacagatat cccaccagag gagaaatacg tagtttattg gccagcatgc cgaggataaa
    21781 ggcgagcaca aggccgccaa caatggtggt gataagcggg gtggcgtgat gcattccgtc
    21841 tccttttcct ggtggttatt gtccattttt ggccgggaaa accaaaatta caggtaatag
    21901 tttatgacaa tttcattgat gatgtttatg aataattgtt gaattttgca gaaaaatgga
    21961 attagctgca aaaaaaagca cggatcggaa aacagaaggc ggtttaacag aggaaaggct
    22021 tatggcgact gtaaaacgat gcagccaaag tttgcctttg gctgcaatga aatcagtctt
    22081 tatgccggtt atcaggcagg aatatggtca acatccccaa tagtggcagg aaagcacaga
    22141 ttttatagac taactctatg ctggtgtggt cggcgataag ccccagaact gccgccccca
    22201 gacctcccat gccgaaagca aaaccgaaaa atagtccaga aaccataccg atacgtcccg
    22261 gaagcagctc ctgggcatag accagaatgg cagagaatgc cgaagcgagg ataaatccaa
    22321 taatcaccgt taaaaccccc gtccagtgca ggctggcgta gggtaaaatc agcgtaaacg
    22381 gcgcaacgcc gaggatagag ccccaaatca catatttccg tccaatttta tcccctacag
    22441 gcccgccaat caccgtacct gccgcaacgg caaacaggaa ggcaaacaaa tgaagctgag
    22501 cattctggat agataatccg aatttttgca tcagataaaa ggtgtaatag ctgctgatgc
    22561 tcgccatata gaaatatttc gagaaaatga ggattaacag aatgctgacc gccagaacaa
    22621 ctttattgcg cggcagtgga ttgataatcg tcgctttggg ttttccttta ttcattcggt
    22681 gctgtgccga gtaccaacgg ctgatttgcg ccaacaccac gatcgccagc agtgccgcaa
    22741 gcacaaacca ggcaacgttg cctttgccat aaggcgcgat aatcaccgcc gccagcaagg
    22801 ggcccaggga actgccaaag ttgccgccga cctgaaatat agattgcgcc aggccatgac
    22861 gcccgccgga agccatacgg gccacgcgag aagattccgg atgaaagacc gatgaaccgg
    22921 taccgaccag cgccgctgcc agcagaactg cgccgaaact gcccgccagc gccagcagca
    22981 ccagaccact taaggtaaag cacatgccaa tcggcaacga ccacggcatc ggatatttat
    23041 cggtccagta gccgaccacc ggttgcagta gcgaagaggc gagctggaag gtgagggtta
    23101 tcatgccaat ctgcataaat gtcagagaaa attctgactg aagcagtgga taaatcgcca
    23161 gaatcagcga ttggatcatg tcgttcagca gatgtgagag gctgatagca cctaaaatac
    23221 caaacgatgt tcgggcctta gtcgttgacg cagccgcgcc cgccacaggc tgggtttgtt
    23281 cactcattgc cataggaaag tcactttttc agggttgcga tgtaaagaat gatcttattt
    23341 gtgattatta cccgactaac atacctgtat gcgccgcctg aaggaagtct caacacggaa
    23401 tacagaattt ctaatctgga tgcagattta tcttcaccgg acgcagactt gtttatgatg
    23461 tcgcgtcata ctatttttca acacgttgaa atcaggtcag ggagagaagt atgaaattat
    23521 tgcagcgggg cgtggcgtta gcactgttaa ccacatttac actggcgagt gaaactgctc
    23581 tggcgtacga gcaggataaa acctacaaaa ttacagttct gcacaccaat gatcatcatg
    23641 ggcatttttg gcgcaatgaa tatggtgaat atggtctggc ggcgcaaaaa acgctggtgg
    23701 atggtatccg caaagaggtt gcggctgaag gcggtagcgt gctgctactt tccggtggcg
    23761 acattaacac tggcgtgccc gagtccgact tacaggatgc cgaacctgat tttcgcggta
    23821 tgaatctggt gggctatgac gcgatggcga tcggtaatca tgaatttgat aacccgctca
    23881 ccgtattacg ccagcaggaa aagtgggcta agttcccgtt gctttccgcc aatatctacc
    23941 agaaaagtac cggcgagcgc ctgtttaaac catgggcgct gtttaagcgt caggatctga
    24001 aaattgccgt tattggcctg acgacggatg acacagccaa aattggtaat ccggaatatt
    24061 tcactgatat cgaatttcgt aagcccgctg atgaagcgaa gctggtgatt caggagctgc
    24121 aacagacaga aaagccagac attattatcg cggcgaccca tatggggcat tacgacaatg
    24181 gtgagcacgg ctctaacgca ccgggcgatg tggagatggc acgcgcgctg cctgccggat
    24241 cgctggcgat gatcgtcggt ggtcactcgc aagatccggt ctgtatggcg gcagaaaaca
    24301 aaaaacaggt cgattacgtg ccgggtacgc catgcaaacc ggatcaacaa aacggcatct
    24361 ggattgtgca ggcgcatgag tggggcaaat acgtgggacg ggctgatttt gagtttcgta
    24421 atggcgaaat gaaaatggtt aactaccagc tgattccggt gaacctgaag aagaaagtga
    24481 cctgggaaga cgggaaaagc gagcgcgtgc tgtacacccc tgaaatcgct gaaaaccagc
    24541 aaatgatctc gctgttatca ccgttccaga acaaaggcaa agcgcagctg gaagtgaaaa
    24601 taggcgaaac caatggtcgt ctggaaggcg atcgtgacaa agtgcgtttt gtacagacca
    24661 atatggggcg gttgattctg gcagcacaaa tggatcgcac tggtgccgac tttgcggtga
    24721 tgagcggagg cggaattcgt gattctatcg aagcaggtga tatcagctat aaaaacgtgc
    24781 tgaaagtgca gccattcggc aatgtggtgg tgtatgccga catgaccggt aaagaggtga
    24841 ttgattacct gacagccgtc gcacaaatga agccagattc aggtgcctac ccgcaatttg
    24901 ccaacgttag ctttgtggcg aaagacggca aactgaacga cctgaaaatc aaaggcgaac
    24961 cggtcgatcc ggcgaaaact taccgtatgg cgacattaaa cttcaatgcc accggcggtg
    25021 atggctatcc gcgccttgat aacaaaccgg gctatgtgaa taccggcttt attgatgccg
    25081 aagtgctgaa agcgtatatc cagaaaagct cgccgctgga tgtgagtgtt tatgaaccga
    25141 aaggtgaggt gagctggcag taatgaaccc cgtcgcggcg tcatgatatg acgccgccat
    25201 ttatgaatat tgaaatcttg catcgtaatc gcgatgtgtc aaaccaaatg ccatcagcat
    25261 ataattctgc ggatcaccag gctgttcttc atctgcttca aaccaaagtg tcacgcctga
    25321 gcgattgacg tggattaaga gtgtatgggg cgattgacgg cggatttgca gatcagcgcc
    25381 ttcgttaaca agcatgttaa atacacgcgt aacgttcaac tcagcgacgt tattcaatat
    25441 tcctttatca aatcggatcg ctttgcccaa atggatttgg ctcagataat cgcaccaaat
    25501 catatatagc ttgttgtttt cgaaatgaaa ctcaacatta ccaaatagcc agatataaaa
    25561 accatttccc atatcatttt tagagtccgg ctcaacaaag ttttgttcca accatgccat
    25621 tgtttgcccc agttcgacaa aatcgaattt tccgttaaag acaaaattgt ggaagctaac
    25681 atcaacaaga tggtcaaatt tcatctttcg ctcctttgtg aattttttac atgcttcaaa
    25741 agccagcgta atttacatcg gtgcaggcga ctctccagtg ttgagcgaaa atttgcgatg
    25801 tcgacattgg accgatctac cgcagcaacc cccgtgtatt gtgggatcgg tgaagggatt
    25861 ttttgtaact ttcccagcgc cagcagttgt tgaatgatcc tgctaccgtc gtggtaatca
    25921 tcgtaaatag ccactggcag aggctgccag gcgggggtcg atttgataaa tccggaaaaa
    25981 aaatacgggt ccagatcgcc gaaatcaaag ccgtaggcga ggggaacttc aatattttgc
    26041 tctgtttttt tattctcaaa tgtacaatga aaaccgtgaa aacaatactg ccactttccc
    26101 attttgcctt ttcgcttacg gttgcatttg aagggggcaa aggtaactag cgggttattt
    26161 tccgccatct caacgttcag ttgttcagcc aatgcaaata taagctctgt cgccagctta
    26221 cgatattctt tcgcgcagtg ttcgaaaaaa tcctgatggc ggttaatctc ttcaagcgat
    26281 attaatctgg cgatcctgat aaccgggtcg gcagaatgca tgccaacaac aaattcatcg
    26341 agctgtgctt tcagcaccag cccgaattcc tcacgatacc tctctgcttc gctggcaaaa
    26401 tgcagatgta ataccttctc tctcgatgtt tgccgaagta aggcgatatc cggtatctca
    26461 tctgcacaag cgatacttaa tgtctctgta atcctttcca catctcatct tccgtgtgca
    26521 tcacttaatc gcgacgggcg atatcagcaa atttggcatc gagtatcttt gccaggtcgc
    26581 ttgccgccag ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt
    26641 cttgtgcggg ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc
    26701 cccctaccag ataccccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct
    26761 tggcacccag cgcttttgct acttttttaa gatccagttg acctgcgacc ggcgtaacag
    26821 ccacggcaag gtgtttcata tcaccgttca ctgcgaccag cagcgttttg tagacctgat
    26881 ccggattcaa acctaatttt ttgacgactt catcgccaaa attggtttca gcaggatcgt
    26941 gctcgtaggt gtggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg
    27001 tcatggtaat tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc
    27061 caaaaaaaag agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc
    27121 gagttacacc gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac
    27181 gggccaatag cgatattggc cattttttta gcgcaacatc tgcggcaaat tcccttctcc
    27241 atacaggtgt agtgcaccga ccgcgaccac atagcgcccc ggcggcatgg cgcgtaattt
    27301 gtcccgccag gcgaggtttc gctgatgcat cagcacatcg tacagcgact gactgaacgt
    27361 attgggcagc gttatatcat tattttgcgg cggtgcattc agccaccagc tcatcatttg
    27421 ttgcagcaac cgtgcgttgg tgtgccagtg ggtcagcgta tcgtccagca gcgccagtcc
    27481 tttgtcaggg agctggagca acatggcaat ctggttttca gccccttcca gttcaatcac
    27541 gggtttatgt tgttgcttag ccgcctgcaa tagctggtaa tcgataccgt attctgcccg
    27601 cagccccagt ttttgcgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg
    27661 ggtagaaaag agcgacggag aaatgcccat ctcctggcta atgtgctgca ggttttggag
    27721 ttggtcctcg ctaatgcgct cttccagggc ctcgcaggca ggcaaattgg caaaaggcgt
    27781 atcgctggtg gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag
    27841 caaacgggtg ggcaggggag ccatatcgtg gctacccata tgaatactgc cgatcagatg
    27901 aaaatggcga ttgccgggaa gagagatatc gatagcaggc caggtgtaat gattaccgcg
    27961 cagcgcggcc caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa
    28021 atatcatgct agcgcgcggg gagggatggc gcaaatggag ttgattgttg cacttgtgca
    28081 cagtgcttgt gtgcattaca atgattaaat aatggacaag gagatgagag aggaatggct
    28141 caaaagaaaa atataaggag ttttcgtgat gcctggttgg ctgatttttt tgtccattca
    28201 acgccacatc gaaagatccc tgctgaaatc cataccacgt tatcacgaaa gttggacatc
    28261 attaatgctg ccacttctca tcgggattta cgctcccctc cggggaaccg atacgaagag
    28321 ttatccggga aattacagga atactcctcc attagagtga ataaacagta ccgactgatt
    28381 tttaaatggg ttaatggcaa ggccgaagaa ttgtttttag acccacacaa ctactaaaac
    28441 atagctgtcg tattgacgag aaaggtaaaa ctatgaaaca ggcaacaaga aaaccgacga
    28501 cacccggaga tattcttctc tatgaatatc tggaaccgct cgatttgaaa atcaatgagt
    28561 tagcagagtt gctgcatgtt catcgtaata gcgtcagtgc actgatcaat aacaatcgta
    28621 aactcaccac tgagatggca ttccgtctgg cgaaagtttt tgataccaca gtcgattttt
    28681 ggctaaacct ccaggcggca gttgatcttt gggaagttga aaacaacatg cgtacccagg
    28741 aagaattggg acggattgaa acagtggctg aatatttggc acgccgtgaa gagcgtgcaa
    28801 aaaaggtcgc gtagaataat ttccctataa cggatgcgcc accctaaagc agcgcatccg
    28861 caatgatgta cttattcctt cggtttaaac cgcagcaatc ggttggcgtt actcactacg
    28921 gtgatcgacg agagcgccat tgccgctccg gcaaccaccg ggttaagcag tgttccagtg
    28981 aacggccaca aaataccggc ggcgaccgga ataccaatgc tgttgtagat aaacgcaccg
    29041 agcagattct gcttcatgtt gcgcagcgtt gcgcgggaaa ttgcaagcgc atccgcaaca
    29101 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    29161 ccacccatcg caataccgac atccgcctga gccagcgctg gcgcgtcgtt aatgccgtcg
    29221 cctaccattg ccacctgacg tccttcactt tgcagacgtt tgatcgcttc ggctttaccg
    29281 tctggcagca cgccggctat cacctcatca atccctgctt ctttggcgat cgcattggcg
    29341 gtggtcgggt tatccccggt tagcatcacc aggcgatatc ccgctttatg caggcgttgc
    29401 agcgccgcca cgctatcact acgcaacgga tcgcgtacag ccagcagggc taccgctttc
    29461 ccgtcaaccg ccagcagcac aggcgttgcc ccttgcgatg cctgagctga aatatctgct
    29521 tcaattgctt tggtatcgac ctgttggtca tttaacagcg cctgattgcc cagcaataac
    29581 gcatgacctt cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    29641 tgcggtagct gcatatcacc tgctttatcg aggatcgccc gtgccagagg gtggctggaa
    29701 ccttgctcca gcgccgccgc cagacgcaat gcttgcgctt catcaatatc agcaaatgtt
    29761 tttactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacactacg
    29821 gtgtcgagcg tactggcgcg ttgcagcgcg tcggcgtccc gtaccagcac gccaaactca
    29881 gccgcccgcc cgacgcccga aataatcgac atcggcgtcg ccagccccag cgcacacgga
    29941 caggcaataa tcagcaccgt ggtggcaatc accagggtat agacaatctg cggtgccgga
    30001 ccaaagaaat accagattgc cgcactgatc agcgcaatca ccaccactac cggcacaaat
    30061 acggctgaga ttttatccgc cagctgaccg atttctggct tactgctctg ggcctggcgc
    30121 accatacgaa taatccgcga cagcgtagta tggctgccaa ccgcactggc gcgaaacagc
    30181 acgctaccgt cctgcaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc
    30241 gggataggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctct
    30301 ccatctaccg gtacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttcc
    30361 gccagtggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg cggagttaaa
    30421 tcgagcaatt tctccagcgc ctttgaagaa cgctgacgtg cgcgcgcttc cagcatatga
    30481 ccgagattaa tcagaccgat aatcatcgca ctggcttcat agtaaagatg ccgcgcttcc
    30541 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacacccgtt
    30601 cccagcgcca ccagcgtatc catcgtcgcc gcaccgttca gcaggctttt ccatgcgctg
    30661 cggtaaaaat gcccgccggc gaaaaccatt accgccaggg ttatcaggcc gataactaac
    30721 cacaggctgc ggttgtcagc ggtgaccatc atgttatcgc cgatcatccc ccagaccatc
    30781 accgggatac ccaccgccag cgcgacaatt gcctgccagc ggaagcgctt catcgtagcg
    30841 acggcggttt cttgctggcg ctcgcggcgt ttagcgtcat cttcaatcgc ttccgcgccg
    30901 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    30961 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcaacgca
    31021 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    31081 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    31141 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    31201 gcttgtttga tggtttcgat tagctgttct gcactggcag tcccggtaac gtgcgcttca
    31261 gtgatagaca catccgcctg ttcaacatcc gggcgctgtt cgagactttc tttcacgcgt
    31321 ttaacgcagt gaccgcagga caggccgtcc agggtcaggt cgatagtttg tgacataaaa
    31381 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    31441 ggtcaagaaa ttaataaacc aggcgggtaa aagtccgtaa agattaaaaa atcggctcga
    31501 tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa
    31561 gttaaatcag gatgcatgaa aatcggcacc ggggtgagga attacctccc gcatctataa
    31621 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    31681 acacccaatt tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga
    31741 atgtccctgg tcaactggcg gcagtggcta tcgtgaccag cgatggcaac gtctatagtg
    31801 cgggtgacag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc
    31861 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa agttggcgct gacccgaccg
    31921 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg ctttcgccac
    31981 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat actgaacaac
    32041 gctggcagcg aattttacat atccaacaac aactggctgg tgagcaggta gcgctctctg
    32101 atgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc
    32161 tgtactcagc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    32221 agtgctccac gctcatcaat actattgaac tggcaacgct tggcgcgacg ctggcggcgg
    32281 gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc cgacaatgtg ccgtacattc
    32341 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg
    32401 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgttcct ggcgtgatgg
    32461 gaattgccgc gttttcacca ccactggacg aagaaggcaa cagtgttcgc ggtcaaaaaa
    32521 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    32581 cacggaaggt aataacggta acaaacccct cggtctatgg aacgtcgttt ccatcggcat
    32641 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga
    32701 agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc
    32761 ctatgcgcgt ctgggggcga gctatcccag caatggcggt attatcgact tctttcgtcg
    32821 cggattaggc aacggcgttt tttcgctggc gctctcgtta ttgtacctgt tgacgctggc
    32881 ggtgagtatc gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    32941 aggcagtcag gaggagcacc ttattttgct ctacgcattg gggatcattg cggtgatgac
    33001 gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat
    33061 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca
    33121 tatttccgtt tctgcgcccc ccagctccgg ggcgttcttc tcctgtatcg ggataacctt
    33181 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgga
    33241 ggtcataatg ccacgggcgt ttctggtggc gattggcgtt accacgttgc tttatatctc
    33301 gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac
    33361 cgccgtagcg caggctgctt ctccgctcct cgggcatgtg gggtatgtga tcgtcgtcat
    33421 cggcgcttta ctggcgacgg cttcagccat caacgcaaac ctgttcgccg tgtttaacat
    33481 catggacaac atgggtagcg aacgcgaact gccgaagcta atgaataaac ctctgtggcg
    33541 gcagagtacc tggggtaaca tcatcgtcgt ggtgttgatt atgctgatga cggcggcgct
    33601 gaatttaggc tcactcgcca gcgttgccag tgccaccttt ttgatctgct atctggcggt
    33661 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    33721 tggtacgttg gttatgttgt tggtgatcgt tggctttatc tacagtctct ggtcccaggg
    33781 cagccgtgcg ctgatatgga ttattggcgc actcttactc agccttattg tggcaatggt
    33841 catgaagcgc aataaaaccg tataacatct ctctgtgcgc agtacctcct gtattattgt
    33901 ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt
    33961 aacaaagcac aggaggcgtt gcgcgaacga tgaacattag cgatgtagca aaaattaccg
    34021 gcctgaccag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    34081 gcagcgaaaa cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc
    34141 gccaggcacg ccaggtgggc tttaacctgg aagagagcgg cgaactggtg aatctgttta
    34201 acgatccgca gcgtcacagc gccgacgtca aacgtcgtac gctggagaaa gtagcggaga
    34261 tcgaacagca tatcgaagaa ctgcaatcca tgcgtaacca gctgctggcg ctggcgaatg
    34321 cctgccccgg tgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    34381 atcatcgggc ggggtgatta aggcttgcga aaatagtcgt ccattatttt cgttagctcc
    34441 ggacgttccc cgtcatcatt gatggcaaaa tccagaaaaa aagcatctgc ttgcggcgtt
    34501 cggttttgcg ccatcaaccg gaaggtgagg gggcctgacc aggtttcgga tgggctgatg
    34561 tgcttatcag ggccgaaact ctcccatgcg ctgggcggta atgttttgca cgcggtgagt
    34621 tttggtatgt ggtattgcag ctctgttttg tgcagacatt ccccgccagg aaataccagg
    34681 gtttgttgct catcattaaa atgggttgcc gtgctgagca ccagtgtttt gagcggtcgc
    34741 ccttccagcg cggcggcagt agccttatgg tgtccgtcga gtagaacgca gagataggaa
    34801 tcagggagat accaggccag accacgggca attccagttt tcgagagcag acaaaaaccg
    34861 ggcggctccc tgttcatcct ccgcaaaacc gctgtagaca aattccgcac aggtagggca
    34921 tccagcctcg gaccagaaac gtattaacgg gacatcctgc cagcagagcg aatggataag
    34981 accgtgcgag tgcggatcgt aaatcacaca ttgacgcaga aatcctttcc cggacttcac
    35041 cgaaaaaatg acgcccgcag cggtattttt cagcagacgt acttcaggtt ttcgaaaggg
    35101 ggaaacatgg ctactcctta gccgaacatt caattaacac tatgtggggg gctatatgcc
    35161 ggatgcggcg taaacgcctt atccggcatg ggttttggta cagatttcca ggtgtgataa
    35221 gacgcgtcag gcgatgaccg cacggataat cagcgttatt ccttctatcg caatcacctc
    35281 aacatgcgta ccagcgccga gatcctcgct ggcgctgaca ggccatgaac tgtcaccgac
    35341 gcgcatatga ccgcgcccgt tgaccagcgg agattccaga acaaaacgtc ggccaatcaa
    35401 ctgctgcccg cgctggttta aatgactgtc gctgtgcttt tgttcgcgca cccgccgcga
    35461 caaccatttc caccacagcc aggcggcgag cagcgtcagg acggcaaaca ttaccccttg
    35521 ccactcccaa cccagcggca gcaaccagac caccagaccg gtaatcaccg ctgccacgcc
    35581 gctccacaac agataaccat tgccgcccaa catctcggct gccagcagca aaccgccgag
    35641 acttagccag aaaatatgtg gatgaacgac cattaactcc atcatggctg agtccgtttg
    35701 ttggcgctgt ctttcaccag ctcagcaatc ccggcaatcg accccatcag gctgctggcc
    35761 tctaatggca tcatcaccac tttgctgtta ctggacgaac cgatctgctg taacgcttcg
    35821 gtgtatttct gtgcgacgaa gtagttcacc gcctgaatat caccggaggc gatggcttca
    35881 gacaccattt tggtggcgcg ggcttctgct tcagcggaac gttcacgcgc ttcagcctgt
    35941 aaaaacgccg actgacgttc gccttccgct ttcaggattt gtgactgttt ttcaccttcg
    36001 gctttgagga tttccgcctg acggatccct tccgcttcaa ggatgtaagc gcgtttggta
    36061 cgttccgctt tcatctgcgc gttcattgaa gagataagct ctgcgggcgg gcgcacatca
    36121 cgaatttcga tacgggtgac tttaatcccc cacgggttgg tggcctcatc gacaatatgc
    36181 agcagacgcg agttaatgct gtcgcgctga gagagcattt catcaagttc cattgaaccc
    36241 agcacggtac ggatgttagt catggtcagg ttgatgatcg ccagctccag attgctgact
    36301 tcataagccg cgcgcggcgc gtcaatcacc tgaataaagc acacagcgtc gatggtaacg
    36361 ttggcgttat ctttcgagat aacttcctgg gaagggatgt cgagcacttg ctccatcata
    36421 ttgatcttgc gaccaatgcg atccataaac ggcaccacca ggcttagacc tggctgtaac
    36481 gttttggtat agcgaccaaa gcgttctacc gtccattgat agccctgcgg cacgattttg
    36541 acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa tcgggataaa gataagcatc
    36601 ggaaaaacct cctgttgtac cgtccataat cagcaaaatt gctgcttgat taaacaaatt
    36661 atacctgatt actgaaagag agttccccct tattcctgcg aaggataaac tgtttttagt
    36721 aaaaatcaga aaaagggaac agcgatgcag gaaaatagtc ctttgcttca gctacaaaac
    36781 gtaggatatc tggcgggtga tacgaagatt cttaataaca tcaatttttc gctgcgtgct
    36841 ggcgaattta agttaattac cggtccttct ggttgtggca aaagtacact gctaaaaata
    36901 gttgcttcat tgatcagccc aaccagcgga acgttactgt ttgaaggtga ggatgtcagc
    36961 acactaaagc cagaaatcta ccgtcagcaa gtctcttact gcgcccagac gccgacgctg
    37021 tttggcgata cggtatacga taacctgatt tttccctggc agatccgtaa ccagcagcct
    37081 gacccggcca tttttctcga ttttcttgaa cgcttcgctt taccggacag cattttgacg
    37141 aagaatatcg ccgagctatc tggtggtgaa aaacaacgca tctcattgat tcgtaacctg
    37201 caatttatgc cgaaggtttt attgctcgac gaaatcacca gtgcgctgga tgaaagtaat
    37261 aaacataacg ttaatgagat gatccaccgt tatgtacgcg agcaaaatat tgctgtgctg
    37321 tgggtgacac acgataaaga cgaaattaat catgcggata aagtgattac actgcaaccg
    37381 catgctggag aaatgcagga agcacgctat gaactcgcat aatattacta acgaatcatt
    37441 agcactggca ttaatgctgg tggtggtggc aatcttaatt agccataaag aaaaactggc
    37501 gctggagaaa gatattctct ggagcgtcgg gcgcgcgata atccagctga ttattgtcgg
    37561 ctatgtgctg aagtatattt tcagcgtgga tgatgccagc ctgacattat tgatggtgtt
    37621 atttatctgc tttaatgcgg cgtggaacgc gcaaaaacgc agtaaatata ttgctaaagc
    37681 ttttatctca tcgtttattg ccattacggt cggggcggga attaccctgg cggtactgat
    37741 cctctctggg tcgattgaat ttatcccgat gcaggtgatc cctatcgccg ggatgatagc
    37801 cggtaacgcc atggtagcgg tggggttgtg ttacaacaat ttagggcaac gggtcattag
    37861 cgaacagcaa cagattcagg agaaactgag tcttggcgcg acgccgaaac aggcttcagc
    37921 gatattgatt cgcgacagta ttcgcgcggc tttaattccg acggtcgatt cagcaaaaac
    37981 ggtaggctta gtgagtttac caggaatgat gtccgggctg atatttgccg ggattgatcc
    38041 ggtgaaggcg attaaatatc agattatggt gacctttatg ctgctctcaa ccgccagctt
    38101 gtcgaccatt attgcctgct atttaaccta tcgtaagttt tacaattcgc gccaccaatt
    38161 ggtggtgacg caattgaaga agaaatgatg cgatgccgga tgcgtcttat cagacctaca
    38221 tggcacctgc aatgtattga attagcacgg tttggtaggc cggataaggc gttcacgccg
    38281 catccggcgg ctatgcgcgg atgcctgatg cgacgctggc gcgtcttatc aggcctacaa
    38341 accagcacag catcgcattt caacggaaaa tcaatacaac aacgcataca gctggcggcg
    38401 atacttcgac gccagtgcat cacccgtacc cagcgcagcg aggatctcct ggaatgtttt
    38461 acgtgtctga ccatctgcgg cggtgagatc tttacgcaga tgcccaaaca gtaactccag
    38521 cgcctcttca ttgcgcccaa cctggtgcag ttgcagtgcc agttgcgtcg ccagtgcggc
    38581 atcttctgga ttctccgcca cctgctgttg caactgttga atttccggcg tatcagccgc
    38641 ctgcttcagc agttcgattt gcgccaccag cccctgatag cgggtgtcct gatcctgcaa
    38701 cggaatggtt ttcagcacgg cttctgcatc ttcagaacgg ttcagcgcaa tcagcgtttc
    38761 tgccaacagc aggccgattt ccccgttctg attcgacaac tgccaggcgt ctttcagcaa
    38821 tggcagggcg tcggtgtaat tgccttcctg catcagttgc atcgcctgct gcgctttcag
    38881 ctcttcttca cgcggcagca ctttatccag cagggcgcgg atcgcctctt ccggttgcgg
    38941 cccctggaag ccgtctactg gttgcccgtt ctggaacaga tatacggtcg gaatcgcgcg
    39001 cagaccaaac tgggcggcaa tcatctgctc cgcgtcgcag tccagcttcg ccagaataaa
    39061 ttgcccgttg tactgcgccg cgaggctttc cagaattggg gttaactgca aacagtgctg
    39121 gctacgttca gaccaaaaat agaacagcac cggagtggtc atcgactgtt caagaacctg
    39181 ttgcaggtta gattcgttaa tgttgacaat attttctacg gacatggagt cgctctctgt
    39241 tgtcgatatt ttctttgaca tgggggctta agcgcgcgct tcaactcacc cctgcaatat
    39301 tttgtccatc acgcgccccg gcagcaggcg cttaagcacc attatcgccc tggtcaccag
    39361 cgtcaccgga tagcgcatct tcggcttctc gctaataaaa gcatggcgca ctttgccgac
    39421 caccgcttcc ggtcccaacg taaagcgggc ggcgatgcca ggattttcga ctggtttatc
    39481 actttgcgtc tggttgacgt tgtcggtgaa gcgagtacga atgggaccag gttcgatcag
    39541 gctgacttta attccgctgt ggcgcagctc catgcgcagt gcgtctgacc acgcctccag
    39601 cgcatattta ctggccgcgt aagcgccacg acccggtgtg gagattaatc ccatcaccga
    39661 tgaggtcatc acaatacgcc cttcacggtg cggcaacatc gcgggtaaca ggcgcatggt
    39721 gagctggtgt gcgccgaaaa agttagcgga aaactgctgt tccatctgcg cacggctgat
    39781 ggtggaaagg gggccataca tgccgaatcc ggcattgtta aagatcccat acagacaatt
    39841 atcggtcagg gcgatcactt cgtcggctgc gcgatcaaca ctttctggcg aatccagatc
    39901 gatcaacacg ccggtaaatc ccatgctgtt catgcgctca acatcatccg gtttccggca
    39961 acctgccagc acatgaaaac cctggcgttt taattcgagt gcgctttcca ggccaatacc
    40021 actggaacat ccggtaatta agaccgattt ttgcataact ttacctgtca ggatctccgt
    40081 tgctttatga gtcatgattt actaaaggct gcaactgctt cgccatccag tcggcaataa
    40141 acggctgggc gtcgcggttg ggatgaatac cgtcatcctg catccattgt ggcttgaggt
    40201 agacctcttc cataaaaaag ggcagcagcg gaacatcaaa ctctttggcg agtttggggt
    40261 aaatggcgct aaaggcttca ttataacggc gaccatagtt tgcaggcaga cgtatttgca
    40321 ttaacaatgg ttcagcgttg gcggctttga catcctgcaa aatctggcgc agcgtttgct
    40381 cggtttgctg tggctgaaaa ccacgcaaac cgtcattgcc gcccagttca accagcaccc
    40441 agcgcggctg atgctgtttc agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt
    40501 caccgctgat gctggcattg actaccgacg ttttactctg ccacttatca ttcaacaagg
    40561 caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca
    40621 ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat
    40681 gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca
    40741 tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact
    40801 ggtgggcgag tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggctggatga
    40861 cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc
    40921 gcgggcaaag ttgcgcgcga agcacgtagg ctttgttttt cagtcattta tgttaattcc
    40981 tacccttaac gcgctggaaa acgtcgagct tccggcgctg ctgcgcggtg agagtagcgc
    41041 agaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtcttga
    41101 tcatcttccg gcacagcttt ctggcggcga acagcaacga gtggcgctgg cgcgagcctt
    41161 taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggtaaccttg accgccagac
    41221 gggcgataaa attgccgatc tgcttttttc cctcaaccgt gagcatggca ccacgttgat
    41281 tatggtgacc cacgacctgc aactggcggc acgctgtgac cgctgcttac ggctggtgaa
    41341 cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg gcgctcgccg
    41401 tcgctattaa ttgtctggct ggcgcttagc ctggcggtgg cctgcgtgct ggcgctgggc
    41461 aatatcagcg atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc
    41521 gatcgggcgt tgcgcagttc gcgcgaagtg ccgcaagcat ggctggagga agcgcaaaag
    41581 cgcggcctga aagttggcaa gcagctgact ttcgccacca tgacctttgc aggcgacacg
    41641 ccgcaactgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cgatctgcaa
    41701 actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg
    41761 gcactgctta acctgaaaac gggcgatacc attgacgtgg gcgatgccac cttgcggatt
    41821 gccggagaag tgattcagga accggattcc ggttttaatc ctttccagat agccccacgc
    41881 ctgatgatga atctggcgga tgttgataaa accggagccg tgcagccggg gagtcgggtc
    41941 acctggcgtt ataaattcgg cggcagcgag aaccagctcg acggctatga gaaatggttg
    42001 ttaccccagc ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg
    42061 gggcgatcga tagaacgttc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg
    42121 ctggcggtgg cggcggtcgc ggtggcgatg aatcattact gtcgcagtcg ctacgatctg
    42181 gtggcgatcc tcaagacgct gggggcaggg cgagcgcaac tgcgtaagtt aatcgtcggt
    42241 cagtggttga tggtgctggt actttcagcc gttaccggtg gggcgatagg cctgttgttc
    42301 gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc
    42361 ctctggccgt ggctgtgggc gctcggcacc atgacggtta tttcgctgct ggtggggcta
    42421 cgaccatacc gcctgttgtt ggcaacgcag cctttacgcg tattacgtaa tgatgtggta
    42481 gcgaacgtct ggccgctgaa gttttatctg ccgattgtca ccgtggtggt tgtgctactg
    42541 ctcgccgggc tgatgggcgg cagtatgctg ctctgggcgg tgctggcggg cgcggtagta
    42601 ctggctttgc tgtgcggtgt cctgggctgg atgctgctga atgtacttcg ccgcatgacg
    42661 ctgaaatcgc tacctctgcg cctggcggtt agccgcctgt tacgtcagcc gtgggcgacg
    42721 ttgagccagc tttcggcatt ttcgctctcc tttatgctgc tggcactgtt gctggtgttg
    42781 cgtggcgatc tgctcgaccg ctggcaacag cagctaccgc cagaaagtcc taactacttt
    42841 ttaatcaaca tcgctacgga acaggttacg ccgctgaaag cgtttctcgc ggaacatcag
    42901 atagtcccgg aatcgtttta tccggtggtg cgggcgcggt tgacggcgat taacgacaag
    42961 ccgacagaag gcaatggaga tgaggcgctc aaccgcgagc tgaatctcac ctggcaaaat
    43021 acgcggcctg atcataaccc gatcgtcgcc ggtaactggc cgccaaaagc cggtgaagtg
    43081 tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggcgatac cgtgactttt
    43141 atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa
    43201 agtctgcggc ctaatttcta ttttattttc ccggaagggg cattagacgg gcaaccgcag
    43261 agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc
    43321 cagttcccga ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg
    43381 ctggagcagg taagtcgggc gctggaagtg atggtggtgc tggtcaccgc ctgcggtatg
    43441 ttgctgttgc tggcacaggt gcaggtggga atgcgtcagc gtcatcagga gctggtggtg
    43501 tggcgcactc tcggtgcggg caaaaaactg ctgcgcacga cattgtggtg tgagttcgcc
    43561 atgctcgggt ttgtatccgg cctggtggcc gcaattggtg cggaaacggc actggcggta
    43621 ctgcaatcga aagtgtttga tttcccgtgg gagccagact ggcgattgtg gattgtgctg
    43681 ccgtgcagcg gtgcgctact gctgtcgctt tgcggcggct ggctgggtgc gcgactgctt
    43741 aagggtaagg cgctattcag gcagtttgtg gggtgatgtg ataaagccgt ttatttctac
    43801 ctggtagata aacggcttta ttattagtgc atcacgtaa
//
LOCUS       DAJOTB010000020        43598 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJOTB010000020 DAJOTB010000000
VERSION     DAJOTB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.020"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            206..1300
                     /gene="gcvT"
                     /locus_tag="OHN28_001119"
     CDS             206..1300
                     /gene="gcvT"
                     /locus_tag="OHN28_001119"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCQ2296554.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1324..1713
                     /gene="gcvH"
                     /locus_tag="OHN28_001120"
     CDS             1324..1713
                     /gene="gcvH"
                     /locus_tag="OHN28_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCQ2296555.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYKALLEDE"
     gene            1832..4705
                     /gene="gcvP"
                     /locus_tag="OHN28_001121"
     CDS             1832..4705
                     /gene="gcvP"
                     /locus_tag="OHN28_001121"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCQ2296556.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPQQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSILAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4871..6304)
                     /gene="bglA"
                     /locus_tag="OHN28_001122"
     CDS             complement(4871..6304)
                     /gene="bglA"
                     /locus_tag="OHN28_001122"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCQ2296557.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6349..6660
                     /locus_tag="OHN28_001123"
     CDS             6349..6660
                     /locus_tag="OHN28_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCQ2296558.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6824..7483
                     /locus_tag="OHN28_001124"
     CDS             6824..7483
                     /locus_tag="OHN28_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCQ2296559.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(7560..8540)
                     /gene="ygfZ"
                     /locus_tag="OHN28_001125"
     CDS             complement(7560..8540)
                     /gene="ygfZ"
                     /locus_tag="OHN28_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCQ2296560.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8783..9049
                     /gene="sdhE"
                     /locus_tag="OHN28_001126"
     CDS             8783..9049
                     /gene="sdhE"
                     /locus_tag="OHN28_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCQ2296561.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9030..9437
                     /locus_tag="OHN28_001127"
     CDS             9030..9437
                     /locus_tag="OHN28_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCQ2296562.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9477..9998)
                     /gene="fldB"
                     /locus_tag="OHN28_001128"
     CDS             complement(9477..9998)
                     /gene="fldB"
                     /locus_tag="OHN28_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCQ2296563.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10110..11006
                     /gene="xerD"
                     /locus_tag="OHN28_001129"
     CDS             10110..11006
                     /gene="xerD"
                     /locus_tag="OHN28_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCQ2296564.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11031..11741
                     /gene="dsbC"
                     /locus_tag="OHN28_001130"
     CDS             11031..11741
                     /gene="dsbC"
                     /locus_tag="OHN28_001130"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCQ2296565.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11747..13480
                     /gene="recJ"
                     /locus_tag="OHN28_001131"
     CDS             11747..13480
                     /gene="recJ"
                     /locus_tag="OHN28_001131"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCQ2296566.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13571..14669
                     /gene="prfB"
                     /locus_tag="OHN28_001132"
     CDS             join(13571..13645,13647..14669)
                     /gene="prfB"
                     /locus_tag="OHN28_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCQ2296567.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14679..16196
                     /gene="lysS"
                     /locus_tag="OHN28_001133"
     CDS             14679..16196
                     /gene="lysS"
                     /locus_tag="OHN28_001133"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2296568.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16239..16787)
                     /gene="idi"
                     /locus_tag="OHN28_001134"
     CDS             complement(16239..16787)
                     /gene="idi"
                     /locus_tag="OHN28_001134"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCQ2296569.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16842..16913
                     /locus_tag="OHN28_001135"
     CDS             16842..16913
                     /locus_tag="OHN28_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296570.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16910..17035)
                     /locus_tag="OHN28_001136"
     CDS             complement(16910..17035)
                     /locus_tag="OHN28_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296571.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17037..18485)
                     /gene="uacT"
                     /locus_tag="OHN28_001137"
     CDS             complement(17037..18485)
                     /gene="uacT"
                     /locus_tag="OHN28_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCQ2296572.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18921..20840
                     /gene="ygfT"
                     /locus_tag="OHN28_001138"
     CDS             18921..20840
                     /gene="ygfT"
                     /locus_tag="OHN28_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCQ2296573.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTSMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20840..21328
                     /locus_tag="OHN28_001139"
     CDS             20840..21328
                     /locus_tag="OHN28_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2296574.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(21364..22731)
                     /gene="ghxQ"
                     /locus_tag="OHN28_001140"
                     /gene_synonym="ygfQ"
     CDS             complement(21364..22731)
                     /gene="ghxQ"
                     /locus_tag="OHN28_001140"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCQ2296575.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22767..24083)
                     /gene="guaD"
                     /locus_tag="OHN28_001141"
     CDS             complement(22767..24083)
                     /gene="guaD"
                     /locus_tag="OHN28_001141"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCQ2296576.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24101..25501)
                     /gene="xanQ"
                     /locus_tag="OHN28_001142"
     CDS             complement(24101..25501)
                     /gene="xanQ"
                     /locus_tag="OHN28_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCQ2296577.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGISYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25666..28536)
                     /locus_tag="OHN28_001143"
     CDS             complement(25666..28536)
                     /locus_tag="OHN28_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCQ2296578.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28533..29312)
                     /locus_tag="OHN28_001144"
     CDS             complement(28533..29312)
                     /locus_tag="OHN28_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCQ2296579.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29363..30691)
                     /gene="ssnA"
                     /locus_tag="OHN28_001145"
     CDS             complement(29363..30691)
                     /gene="ssnA"
                     /locus_tag="OHN28_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCQ2296580.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFTRQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIVLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30694..33792)
                     /gene="ygfK"
                     /locus_tag="OHN28_001146"
     CDS             complement(30694..33792)
                     /gene="ygfK"
                     /locus_tag="OHN28_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCQ2296581.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKSKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34114..34692)
                     /gene="mocA"
                     /locus_tag="OHN28_001147"
     CDS             complement(34114..34692)
                     /gene="mocA"
                     /locus_tag="OHN28_001147"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCQ2296582.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34796..35566
                     /gene="yqeC"
                     /locus_tag="OHN28_001148"
     CDS             34796..35566
                     /gene="yqeC"
                     /locus_tag="OHN28_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCQ2296583.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            35614..37239
                     /locus_tag="OHN28_001149"
     CDS             35614..37239
                     /locus_tag="OHN28_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCQ2296584.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLHQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37280..38212)
                     /gene="arcC"
                     /locus_tag="OHN28_001150"
     CDS             complement(37280..38212)
                     /gene="arcC"
                     /locus_tag="OHN28_001150"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2296585.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38260..39645)
                     /gene="hyuA"
                     /locus_tag="OHN28_001151"
     CDS             complement(38260..39645)
                     /gene="hyuA"
                     /locus_tag="OHN28_001151"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCQ2296586.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39698..40909)
                     /locus_tag="OHN28_001152"
     CDS             complement(39698..40909)
                     /locus_tag="OHN28_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCQ2296587.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40967..42163)
                     /gene="dpaL"
                     /locus_tag="OHN28_001153"
     CDS             complement(40967..42163)
                     /gene="dpaL"
                     /locus_tag="OHN28_001153"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCQ2296588.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42221..43408)
                     /gene="ygeW"
                     /locus_tag="OHN28_001154"
     CDS             complement(42221..43408)
                     /gene="ygeW"
                     /locus_tag="OHN28_001154"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCQ2296589.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10570 a        10951 c        11472 g        10605 t
ORIGIN      
        1 ttattctgtt acgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc
       61 ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg
      121 accgggagtg gctccgatgc tgggtttcgt ggtgataatt tcgccataga aaagttgtca
      181 gccccgctta ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac
      241 gctttgcggc gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc
      301 gcaaatcgac gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat
      361 gaccatcgtc gatcttcgcg gcagccgcac ccgggagttt ctacgttatc tgctggcgaa
      421 cgatgtggcg aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc
      481 tggcggtgtg atagatgatc tcatcgtcta ctactttact gaagatttct tccgcctcgt
      541 tgttaactcc gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt
      601 cggcatcgaa attactgttc gtgatgacct ttccatgatc gccgtacaag ggccgaatgc
      661 gcaggcaaaa gctgccacac tgtttaatga cgcccagcgt caggcggtgg aagggatgaa
      721 accgttcttt ggcgtgcagg cgggcgatct gtttattgcc accaccggtt acaccggtga
      781 agcgggctac gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gcgcgctggt
      841 ggaagctggc gttaagccat gtggcctggg cgcgcgtgac acgctgcgtc tggaagcggg
      901 catgaatttg tatagccagg agatggacga aactatttct cctttagccg ccaatatggg
      961 ctggactatc gcctgggaac cggcagatcg tgactttatc ggtcgtgaag ctctggaagc
     1021 gcagcgtgaa catggcacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct
     1081 gcgtaatgaa ctgccggtac gttttactga tgcgcagggc aaccagcatg aaggcattat
     1141 caccagcggt actttctccc caacgctggg ttatagcatt gcgctggcgc gcgtgccgga
     1201 aggtattggt gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac
     1261 gaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt tggagattga
     1321 ttgatgagca acgtaccagc agaactgaaa tacagcaaag aacacgaatg gttgcgtaaa
     1381 gaagccgacg gcacttacac cgttggcatc accgaacacg ctcaggagct gttaggcgat
     1441 atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg
     1501 gttgccgaat cggtaaaagc ggcgtcagat atttatgcgc cagtaagcgg tgaaatcgtg
     1561 gcggtaaacg atgcactgag cgattctccg gaactggtca acagcgaacc gtatgcaggc
     1621 ggctggattt tcaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgca
     1681 accgcataca aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag
     1741 gatcagggtg aggaacattt acgcctcacc ctcactctct tcgtaaggag agaggttcac
     1801 aattcactgc acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa
     1861 cagcggcgct tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct
     1921 gaatgccgtt ggcgcacaat cgttaaacgc gctgaccggc cagattgtgc cgaaagatat
     1981 tcagcttgcg actccgcagc aggttggcgc accggcgacc gaatacgccg cactggcaga
     2041 actcaaggct attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac
     2101 cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac
     2161 cgcgtatact ccgtatcaac ctgaagtctc ccagggccgc cttgaagcac tgctcaactt
     2221 ccagcaggta acgctggatt taaccgggct ggatatggcc tctgcttctc ttcttgacga
     2281 agccaccgct gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc
     2341 caaccgcttc tttgttgctt ctgacgttca tccgcaaacg ctggatgtgg tccgtactcg
     2401 tgccgaaacc tttggttttg aagtgattgt cgatgatgcg caaaaagtgc tcgaccatca
     2461 ggacgtcttc ggcgcgctgt tacagcaggt cggcactact ggtgaaattc acgactacac
     2521 tgcgcttatt agcgaactga aatcacgcaa aattgtggtc agcgttgccg ccgatattat
     2581 ggcgctggtg ctgttaactg cgccaggtaa acagggcgcg gatattgttt ttggttcggc
     2641 gcaacgcttt ggcgtaccga tgggctacgg tggcccacac gcggcattct ttgcggcgaa
     2701 agacgaatac aaacgctcaa tgccgggccg tattatcggt gtatcgaaag atgcagctgg
     2761 caacaccgct ctgcgcatgg cgatgcagac tcgtgagcaa catattcgtc gtgaaaaagc
     2821 gaactccaac atttgtacat cccaggtctt gctggcaaac attgccagtc tgtatgccgt
     2881 ttaccacggt ccggttggcc tgaaacgtat cgctaaccgc atccaccgtc tgaccgatat
     2941 cctggcggcg ggcctgcaac aaaaaggtct gaagctgcgc catgcgcact atttcgatac
     3001 tttgtgtgtg gaagtggccg acaaagcggg cgtgctggcg cgtgccgaag cggctgaaat
     3061 caacctgcgt agcgatattc tgaacgcggt tgggatcacc cttgatgaaa ccactacgcg
     3121 cgaaaacgtg atgcagcttt tcagcgtgct gttgggcgat aaccacgggc tggacatcga
     3181 cacactggac aaagacgtgg ctcacgacag tcgctctatt ctggctgcga tgctgcgcga
     3241 cgacgaaatc ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg
     3301 ctatatgcac tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg
     3361 ttcctgcacc atgaaactga acgccgccgc cgagatgatc ccgatcacct ggccggaatt
     3421 tgccgaactg cacccgttct gcccgccgga acaggctgaa ggttatcagc agatgatcgc
     3481 gcagctggct gagtggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa
     3541 ctctggcgca cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg
     3601 caacgaaggg catcgcgata tctgcctgat cccggcttct gcgcacggca ctaacccggc
     3661 ttccgcgcat atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggcaacat
     3721 cgatctgact gatctgcgcg cgaaagccga acaggcgggc gataacctct cctgtatcat
     3781 ggtgacctat ccttccaccc acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt
     3841 cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg aacatgaacg ctcaggttgg
     3901 catcacctcg ccgggcttta ttggtgcgga cgtttcccac cttaacctgc ataaaacttt
     3961 ctgcattccg catggcggtg gtggtccggg tatgggaccg atcggtgtga aagcgcatct
     4021 ggctccgttt gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg
     4081 cgcggtttct gcggcaccgt tcggtagtgc ctctatcctg ccaatcagct ggatgtacat
     4141 ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag gtggcaattc ttaacgccaa
     4201 ctatattgcc agccgtctgc aggatgcctt cccggtgctg tataccggtc gcgacggtcg
     4261 cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga
     4321 gctggatatt gccaagcgcc tgatcgacta cggtttccac gcgccgacca tgtcgttccc
     4381 ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg
     4441 ctttatcgat gcgatgctgg ctatccgcgc agaaattgac caggtgaaag ccggtgtctg
     4501 gccgctggaa gataacccgc tggtgaacgc gccgcacatt cagagcgaac tggtcgccga
     4561 gtgggcgcat ccgtacagtc gtgaagttgc agtattcccg gcaggtgtgg cagacaaata
     4621 ctggccgacc gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt tctgctcctg
     4681 cgtaccgatt agcgaatacc agtaatttac tgattcgact atcttctaaa ggcgcttcgg
     4741 cgcctttttc gcttgttaat aaagtgcgct ttgtgtgttt cggttgaggc gtaatataaa
     4801 atattgcgag ctcaatatat taaaagagtg ccaggcctga tgagcggagc acatcaggca
     4861 catcggctaa ttacaaattc tcgccgttgc tggcaatcac ctctttgtac cagttaaagc
     4921 tcttcttacg tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa
     4981 agccgtagcg tttgctgtac tgcccggtag tgaacgacac gcagtcgatg cagccccacg
     5041 gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc ttttttcatc tcttcaatat
     5101 gggcgcgcag gtagtcaatg cggtaatcgt cattgatgct gccatcatct tccactttgt
     5161 cgtaagcgcc aaaaccgttt tcgacaataa acagcggctt ctggtaacgc tcatacagct
     5221 cgcaaagcgc gtaacgcaga ccaaccggat caatctgcca gccccagtca gacgctttaa
     5281 catacgggtt cggtacgctg ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg
     5341 ccttcaccgc gttggtcatg taatagctga agccgagata atcgcaggtg ccttcgcgca
     5401 gtacatccag atcgccgtct tccattttga tgttaaatcc gcggcgttcc cactcgttca
     5461 acacatagga cgggtaatag ccacgcaact gcacatcggt aaagacgtaa cgttcgcgca
     5521 tcgacttctg ggcaaacatc acatcgtccg ggttacagga gtagggatag agcggcacca
     5581 tcgccagcat acaaccgact ttcatctccg ggttgatgcg atgcgcggct ttcaccgcca
     5641 gggcgctggc gacaaactgg tgatgcagca cctgatacat ggtctcttcc gggttttcat
     5701 gttcggtata caccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct
     5761 ggttgttaat ttcgttgaag gtcatccagt atttgacttt gtgcttatag cgttcgaata
     5821 ccacttccgc gaaacggaca aagaaatcaa ccactttacg gttggtccag ctaccatatt
     5881 gctgcaccag atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt
     5941 atttcagcag ttcatcgatc atatcatcgt agaacttcag cccttcttcg tttggctgag
     6001 cttcatcgcc ttttggaaaa atgcgcgtcc aggcgatgga agtacgaaaa catttgaagc
     6061 ccatttcggc aaatagcttg atgtcctcct tgtagtgacc ataaaaatca acggcttcat
     6121 ggtttggata gtatttgcca ggcacaactt ctttggtgat ttcgcgcggc acgccgtgtg
     6181 cgccaccggt cagaacgtca caaatgctcg gccctttgcc gcctttgttc cagccgcctt
     6241 cgacctgatg agcggcaact gcgccgcccc ataagaaatc tttcggtaag gtgagttttt
     6301 tcactatcat aggctccaga tgagtcaatt cttacaggaa atattaccat gcagccaaac
     6361 gacatcactt tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc
     6421 atccgcgacg agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cggacgtttt
     6481 gaagatgacg gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat
     6541 acgctgacgg aaaaacatgc agagcaggaa aatatgaccc tgactgaact gaaaaaggtc
     6601 attgccgaca tctatcccga ccagacacag ttttatgtga ttgaatttaa atgtctttaa
     6661 attgaactca cgcctgttag ttgaaatttc gcatttaatt tcatgatttt tcggagatta
     6721 atctcaaatc agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc
     6781 tggagaggta tacatgtgcg tgtacgtttt tccggagtaa gttatggttc agaagcccct
     6841 cattaagcag ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg
     6901 gctggtgttt ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc
     6961 cagcgccacg gcgataacca gttacagcct ctatggcggc agtatgatcc tgctgttcct
     7021 cgcttcgacg ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt
     7081 tgaccactgc gctatttatc tgttgattgc cggaacctac acgccgtttt tgctggtggg
     7141 gctggattct ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg
     7201 tattctgttt aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct
     7261 ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg
     7321 cagcgttacc ttactggcgg taggcggtgt ggtttattcg ctcggggtga ttttctacgt
     7381 ctgcaagcgc attccataca atcatgccat ctggcacggc tttgtgctcg gcggtagtgt
     7441 gtgccacttt ctggcgatct atttgtatat tgggcaggtg taattggcgt gcctttgtag
     7501 gcctgaaaag acacaccagt gtcgcatcag gcgataaatg cctgatgcga tacagagatt
     7561 tactcttcca gcgaatatgg cagtggctca atacgcaatg tattcgcatc gtcacgcacg
     7621 cggaagatgc tgtccggttc catatcgtta ttcatgacaa cctgcaccac aacctgacca
     7681 tcttccagtt tgaccgcagc cagcaccgta ccggtacgac gccagttctc gcccattttc
     7741 agctctaagt cttcaccagc ttccggcagt cggctggcgc tacccgtcag caaccagagc
     7801 gcgcgtttgt tggcaccacg gaattttgct cgcgccacca tctcttgtcc ggtatagcag
     7861 cctttcttaa agctgatgcc gcccagcgcc tggaggttgg tcgcctgggg gataaactgc
     7921 ccgctgttgg cggcatcaat caccggaaaa cccgcttcaa tgtttaatgc cagccactgt
     7981 tggctattgt tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt ggcagtagct
     8041 tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa accatagcaa cgtggtcgcg
     8101 ccttctttga ctacctgttt ttctctcgaa ggtagttcac taaagagatt tgccaacgcg
     8161 gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc
     8221 actttagaga acaccgcata ttttttcagt tcagtcagct gcggttcacg cacgctgcgc
     8281 cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac gtaaattgct ccacatttta
     8341 cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt cagtcatctg gctgacatct
     8401 gctgtcacct ggccttgcat atatttttcg ctgtccgcgc cagtaatagt ggcaagcgcc
     8461 cagtcatcaa gcgtcatcag cgtcagtggc aaacgggcag aagccgtagg ctgacgggga
     8521 ggaaaaggtg taaaagccat aatagagtcc tgagttgctt aacggcgtat taatcactaa
     8581 tggtaaaaga gctatgaggc aatgcaagtg ctttcactgc gcgatctgtg ccttctgcga
     8641 ggcgacttcc aaaaaaatga agcccttgaa ttgttgtgaa ttaatttcgc gatcgcgatt
     8701 gtgtttactg atattcccgt aaaagaagtc gaaaaacacg ttacaatagc cgggtactgg
     8761 attttcgtga gacaggaaga agatggacat taacaacaaa gcccgcattc attgggcatg
     8821 ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg tttttcgaac atgagtacga
     8881 cagcttaagc gatgacgaaa aacgcacctt tattcgtctg ctggaatgtg acgatccgga
     8941 cctgtttaac tggctgatga atcacggtaa accagccgat gcagaactgg aaatgatggt
     9001 ccgactcatc cagacacgga accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct
     9061 cctggcgcgc acagtggctt tccttgctga ttcatgggct ggttgccgct gttattttac
     9121 tcattccctg gccgctcagt tacaccccgt tatggatggt gttactttcg ctggtggtgt
     9181 ttgattgcgt tcgcagccag cggcgcatta atgctcgcca gggggaaatt cgcttgttga
     9241 tggacgggcg tttgcgttgg caagggcagg agtggagcat cgtcaaagcg ccgtggatga
     9301 ttaagagcgg catgatgctg cgtttacgtt ctgacagcgg aaaacggcaa catttatggc
     9361 tggcagctga cagcatggac gaagccgaat ggcgggattt acggcggata ttgttgcaac
     9421 aagagacgca aagataaaga tgacggcaaa gcatatgtgc taagccgtca gtgacatcag
     9481 gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc agctttgaat acgctcgtcg
     9541 ctaaggtcat actggttagt ttcatccagc gccagaccca cgaacagttg cccgtcagca
     9601 atcaccggtt tcggactggt aaattcatat ccttccgttg gccagtagcc gacgaacttc
     9661 acgcctttgg tcgagagttt gtcatgcagc ataccgagcg catcgaggaa ccactcgccg
     9721 tatcccagtt gatcgccaag cccatacagc gcaacaattt taccttcgag gttcaggtcg
     9781 tcgagctgat cccagacggc ttcccagtct tcctggattt cgccaaaatc ccaggtcggg
     9841 atacccagaa tgagcacatc gtactgctcc attaatttcg gggagtcgtc cttgaggtta
     9901 tgtaaggtca ccagttctgg gccgataata tcgcggattt tttctgccgc catttcggtg
     9961 taacaggtgc tggaaccgta aaaaagaccc atattcataa cgtaagcttc tcgattctgc
    10021 tgtggctttt gcggctagtg tatcagaatc gctttagtca cggcacaata aggcataatg
    10081 gatcaaatga gtgaccataa ggggcgcaag tgaaacagga tctggcacgc atcgagcagt
    10141 ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc
    10201 gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc
    10261 aaagtgacga tttgcaggca ttactggcag aacggctgga gggcgggtat aaagccacca
    10321 gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt
    10381 ttcgtgaaga tgatcccagt gcgcatctcg cttcaccgaa attgccccag cgtttgccaa
    10441 aagatttaag cgaagcgcag gtcgaacgtt tattacaggc accattaatt gatcagccac
    10501 tggagctacg cgataaagcc atgcttgaag tgttgtatgc taccggactg cgtgtctctg
    10561 aactggtcgg gctgacaatg agtgatatca gcctgcgtca gggcgtggtg cgggtcattg
    10621 gtaaaggcaa caaagagcgc ctggtgccgt taggtgaaga ggcggtttac tggctggaaa
    10681 cctatctgga acatgggcgt ccgtggctgt tgaatggtgt gtcaattgac gtgttgtttc
    10741 ccagccagcg tgcgcagcag atgacgcgac agaccttctg gcaccgcatt aaacattatg
    10801 ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg
    10861 ccactcactt attaaatcat ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca
    10921 gcgatctctc caccacgcaa atttatacgc atgtcgctac cgagcgtctg cgacaacttc
    10981 atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac aggaaaattt atgaagaaag
    11041 gttttatgtt atttaccttg ttggcagcgt tttcaggctt tgttcaggct gatgacgcgg
    11101 caattcaaca aacgttagcc aaaatgggca tcaaaagcag cgatattcag cccgcgcccg
    11161 tagctggcat gaagacagtt ctgactaaca gcggtgtgtt gtacatcacc gatgatggta
    11221 aacatatcat tcaggggcca atgtatgacg ttagtggcac ggctccggtc aatgtcacca
    11281 ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcgc
    11341 cgcaggaaaa acacgtcatc accgtgttta ctgatattac ctgtggttac tgccacaaac
    11401 tgcatgagca aatggcagac tataacgcgc tggggatcac cgtgcgttat cttgctttcc
    11461 cgcgccaggg gctggacagc gatgcagaga aagaaatgaa agctatctgg tgtgcgaaag
    11521 ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg
    11581 acgtagatat tgccgaccat tacgcacttg gcgtccagct tggcgttagc ggtactccgg
    11641 cagttgtgct gagcaatggc acacttgttc cgggttacca gccgccgaaa gacatgaaag
    11701 aattcctcga cgaacaccaa aaaatgacca gcggtaaata attcgcgtga aacaacagat
    11761 acaacttcgt cgccgtgaag tcgatgaaac ggccgacttg cccgctgaat tgcctccctt
    11821 gctgcgccgt ttatatgcca gccggggtgt gcgtagcgcg caggaactgg aacgcagtgt
    11881 taaaggcatg ctgccctggc agcaactgag cggcgtcgaa aaggccgttg agatccttta
    11941 caacgctttt cgcgaaggaa cgcggattat tgtggtcggt gatttcgacg ccgacggtgc
    12001 caccagcacg gctctaagcg tgctggcgat gcgctcgctt ggttgcagca atatcgacta
    12061 cctggttcca aaccgtttcg aagacggtta cggcttaagc ccggaagtag tcgatcaggc
    12121 tcatgcccgt ggcgcgcagt taattgtcac ggtggataac ggtatttcct cccatgcggg
    12181 ggttgaacac gctcgttcgt tgggcattcc ggttattgtt accgatcacc atttgccagg
    12241 cgacacatta cccgcagcgg aagcgatcat taaccctaac ttgcgcgact gtaatttccc
    12301 gtcgaaatca ctggcgggcg ttggcgtggc gttttatctg atgctggcgc tgcgcacctt
    12361 tttgcgcgat cagggctggt ttgatgagcg cggcatcgca attcctaatc tggcagaact
    12421 gctggatctg gtagcgttgg ggacagtggc ggacgtcgtg ccgctggacg ctaataatcg
    12481 cattctgacc tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc cggggattaa
    12541 agcgttgctg gaagtggcca atcgcgatcc acaaaaactc gccgccagcg atttaggttt
    12601 tgcgctgggg ccacgtctca atgctgccgg gcgactggac gatatgtccg ttggcgtggc
    12661 gctgttgctg tgcgacaaca tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct
    12721 aaaccagacg cgaaaagaga tcgaacaggg aatgcaagtt gaagccctga ccttgtgcga
    12781 gaaactggag cgcagccgcg acacgctacc cggcgggctg gcaatgtatc accccgaatg
    12841 gcatcagggc gttgtcggta ttctggcttc gcgcatcaaa gagcgttttc atcgtccggt
    12901 tatcgccttt gcgccagctg gtgatggtac gctgaaaggt tccggtcgct ccattcaggg
    12961 gctgcatatg cgtgatgcgc tggagcggct agacacgctt taccccggca tgatgctcaa
    13021 gtttggcggt catgcgatgg cggcgggttt gtcgctggaa gaggagaaat tcgaactctt
    13081 tcaacaacgg tttggcgaac tggttactga gtggctggac ccttcgctat tgcaaggcga
    13141 agtggtgtcg gacggtccgt taagtccggc cgaaatgacc atggaagtgg cgcagctgtt
    13201 gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg ctgtttgatg gtcatttccg
    13261 tctgctgcaa cagcggctgg tgggcgaacg tcatttgaaa gtcatggtcg aaccggtcgg
    13321 cggcggtcca ctgctggacg ggattgcttt taatgtcgat accgccctct ggccggataa
    13381 cggcgtgcgc gaagtgcaac tggcttacaa gctcgatatc aacgagtttc gcggcaaccg
    13441 cagcctgcaa attatcatcg acaatatctg gccaatttag cgtcatcttc tctataaaaa
    13501 agagcgtgga ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag
    13561 aaatcagacc atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg
    13621 ctccgacgtt cttagggggt atctttgact acgacgccaa gaaagagcgt ctggaagaag
    13681 taaacgccga gctggaacag ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg
    13741 gtaaagagcg ttcctccctc gaagccgttg tcgacaccct cgaccaaatg aaacaggggc
    13801 tggaagatgt ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta
    13861 acgaagccgt tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc
    13921 gtatgttctc tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg
    13981 gcggtacgga agcacaggac tgggcgagca tgcttgagcg tatgtatctg cgctgggctg
    14041 aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta
    14101 aatccgtgac gatcaaaatc tccggcgatt acgcttatgg ctggctgcgt acagaaaccg
    14161 gcgttcaccg cctggtgcgt aagagcccgt ttgactccgg cggtcgtcgc cacacgtcgt
    14221 tcagctccgc gtttgtttac ccggaagttg atgatgatat cgatatcgaa atcaatccag
    14281 cggatctgcg catcgacgtt tatcgcgcat ccggcgcggg cggtcagcac gttaaccgta
    14341 ccgaatctgc ggtgcgtatt actcacatcc caaccgggat cgtgacccaa tgccagaacg
    14401 accgttccca gcacaagaac aaagaccagg ccatgaagca gatgaaagcg aagctttatg
    14461 aactggagat gcagaagaaa aatgccgaga aacaggcgat ggaagataac aaatccgaca
    14521 tcggctgggg cagccagatt cgttcttatg tccttgatga ctcccgcatt aaagatctgc
    14581 gtaccggggt agaaactcgc aacacgcagg ccgtgctgga cggcagcctg gatcaattta
    14641 tcgaagcaag tttgaaagca gggttatgag gaaccaacat gtctgaacaa cacgcacagg
    14701 gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga
    14761 acctgcgtga gcaggggatt gccttcccga acgatttccg tcgcgatcat acctctgacc
    14821 aattgcacgc agaattcgac ggtaaagaga acgaagaact ggaagcgctg aacatcgaag
    14881 tcgccgttgc tggccgcatg atgacccgtc gtattatggg taaagcgtct ttcgttaccc
    14941 tgcaggacgt tggcggtcgc attcagttgt acgttgcccg tgacgatctg ccggaaggcg
    15001 tttacaacga gcagttcaaa aaatgggacc tcggcgatat cctcggcgcg aaaggtaaac
    15061 tgttcaaaac caaaaccggc gaactgtcta tccactgtac cgagctgcgt ctgctgacca
    15121 aagcactgcg tccgctgccg gataaattcc acggcttgca ggatcaggaa gcgcgctatc
    15181 gtcagcgtta tttggatctc atctctaacg atgaatcccg caacaccttt aaagtgcgct
    15241 cgcagatcct ctctggtatt cgccagttca tggtgaaccg cggctttatg gaagttgaaa
    15301 cgccgatgat gcaggtgatc cctggcggtg ccgctgcgcg tccgtttatc actcaccata
    15361 acgcgctgga tctcgacatg tacctgcgta tcgcgccgga actgtacctc aagcgtctgg
    15421 tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt
    15481 ccgtacgtca taacccagag ttcaccatga tggaactcta tatggcttac gcagattaca
    15541 aagatctgat cgagctgacc gaatcgctgt tccgtactct ggcacaggat attctcggta
    15601 agacggaagt gacctacggc gacgtaacgc tggacttcgg taaaccgttc gaaaaactga
    15661 ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga catggcggat ctggacaact
    15721 tcgactctgc gaaagcgatt gctgaatcta tcggcatcca cgttgaaaag agctggggtc
    15781 tgggccgtat cgttaccgag atcttcgaag aagtggcaga agctcatctg atccagccga
    15841 ccttcattac tgaatatccg gcagaagttt ctcctctggc gcgtcgtaac gacgttaacc
    15901 cggaaatcac agaccgcttt gagttcttca ttggtgggcg tgaaatcggt aacggcttta
    15961 gcgaactgaa tgacgcggaa gatcaggcgc agcgcttcct ggatcaggtt gccgcgaaag
    16021 atgcaggtga cgacgaagcg atgttctacg acgaagatta tgtcaccgca ctggaacatg
    16081 gcttaccgcc gacagccggt ctgggaattg gtatcgaccg tatggtgatg ctgttcacca
    16141 acagccatac catccgcgac gttattctgt tcccggcgat gcgtccggta aaataagcat
    16201 tacgttatgc tcacaacccc ggcaaccgtc ggggtttttt atttaagttg ggtaaatgca
    16261 gataatcgtt ttctggcttc gcgatttgtc gcctgcatca ccatccacgg actgaacgcc
    16321 cacggcgtag catcaatacc gcgtaaaacc gctgctaaat cacaccattg ataatccatc
    16381 acttcatcat cgttgatctg taacgcactg gtcgtgcgtg cggcaaatac cggacacact
    16441 tcattttcca caatgccatt cggatcggtg gcgcggtagc gaaagtcagg atagatagat
    16501 tcaggaggcg taatttctac gccaagctca taacggcaac ggcggatcac cgcctcttcg
    16561 ttgctttctc ccagttgtgg gtgcccacaa accgagttag tccacacgcc aggccatgct
    16621 tttttgctaa gggcgcggcg ggtaactaat aattgccctt tggcattaaa cagccaactg
    16681 gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc
    16741 gtgggaactc cctgtgcatt caataaaatg acgtgttccg tttgcataat ttctcacatg
    16801 taattctgat cactcatcat tatagataat agaagagtga aatgattaac caagtgagcg
    16861 tttatcgaca accgcccgtt ttgagcggat gtcgacaggt aaaaaccatt taaaaaagca
    16921 gcatcatata tttgaagcga ctttcaatgg ccattgcaac acaatcagaa atgacaggaa
    16981 tagagagagt aaaaagcaac agcagactga atatagcgcg cattaaaaga ttcatattat
    17041 tctccatgct catttttctt cagcttgcgc atcagaagcc acatacgtac tgtcctgact
    17101 tttaaatctt tatcagagac ggattccttc accaggtcag catgatgctg gtagccatta
    17161 aagaagacgt tcagcacaac ggcgctaagg gttgcgagca taatgccgct atgcagcaac
    17221 ggttgcagta cggccggtaa cttagaaaag aaatcgtgag agagcgttgg cgtcatgcca
    17281 acaccgagac tgatcgccac aatatagagg ttgtaacggt tagtggtgta gttacagcgc
    17341 gacagaattc gaatccctgt cgccagtacc atgccgaaca tcaccagccc cgcgccgccc
    17401 agcacaaatt gcggaatgga ggcgaccagc accgccattt tgggcaccat gccaaacagg
    17461 attaatataa ttcccgaaga aatacacacc cagcggctgt ggacgcgcgt cacgctgacc
    17521 agcccgacgt tctgcgaaaa tgacgtatgt gggaagctgt taaacgtgcc tccaatcatt
    17581 gtcccgaccc catctacgcg taacccacga ataatgtcct gggaagagag tttgcgaccg
    17641 actatttcac ccagtgccag gaacatcccc attgactcaa taaatacgat gatgagtact
    17701 gcggtcatgg tcaaaatgga aacgggatcg aaaatcggca taccaaatga catcggcgtg
    17761 acaatcgcaa accatgaagc atcatgtagc ccggataaat tgacttcatt catcatccac
    17821 gaaagtaaaa agccaaatac aatccccagt aatacggcga cgttggacat aaatcctttc
    17881 gcatagcgag taatgagcaa gataaaaatt aagacggcaa aggagatacc taaataaacg
    17941 ggattaccat attgcggatt ccctttacct ccggcggccc agtcaatacc cacctgaatg
    18001 atgctaagcc cgatagaagt aatcaccaca ccggtaacca gtggcgggaa taaaggcatc
    18061 aagcgaccga taagtggcgc taataatgtg gtgataaaac ccgcggcgat agtggcaccg
    18121 aatatcccca gcaggccgat atccgggttc ataccaatgg ctatcattgg tgttacagcg
    18181 gcgaaggtca ccgacataat caccggcagg cggatcccca taaagcggcc gataccgata
    18241 cattgcaata atgtgacgat cccgcagcaa aagagatccg agctaatgag catcgcaata
    18301 gcttcttttg ataggccgag tcggtcacca atcataagag gaacagcgac tgcacctgcg
    18361 tacatgacaa ggacgtgttg cagaccgagt atgatcagct ttcctggtga taatatgcgg
    18421 tcaacctcat cagttgggcg gtcttgccca gaagatgagg gaagttggga atctatggcg
    18481 ctcatcagtg tttctccttt cgcccgccag agtaaaaagc aaccgtttat gttcatgaaa
    18541 ctgtctaaac acatgctcgt ggagtgccag tttttgcgag gaaggggagt tagagacagg
    18601 aattgcggag gcaatcacat aatcgtggtt aaaccacgaa aatgagaaga gaacctgaca
    18661 atggaattat ctgtaatttt ctcatattga tatttcatta atcattctgg catgtcgcac
    18721 tctcgcattt aatcgttttt atctggatag cattcttttg atcggcaatg ttgaaataat
    18781 gaaagcggca tagattgagg ggcaataatt tgaactcagt tgcaaaatta taattaaaac
    18841 ggtcatgcta cctaatatat gcccgtttat tgcatcgcga aatttgagaa gacataactc
    18901 attaaatgaa agggatgcaa atgaataagt ttatcgctgc tgaagctgcg gaatgtatag
    18961 gctgccatgc ttgtgaaatt gcctgtgcgg tggcacataa tcaagaaaac tggccgctga
    19021 gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa aggccaggct gcgaatccgg
    19081 tggcctgcca tcactgcaac aatgcccctt gcgttacggc ttgtccggtt aatgctctga
    19141 ctttccagtc cgatagcgta caactggacg agcaaaaatg tattggttgt aaaagatgcg
    19201 caatcgcttg cccctttggc gtcgttgaga tggtcgatac gattgcacag aaatgcgacc
    19261 tttgtaacca gcgcagttcc ggcacgcaag cctgtattga cgtctgccca acacaggcgt
    19321 tacggctgat ggacgataaa gggttgcagc agataaaggt ggcccgccag cgcaaaacgg
    19381 cagcaggaaa agcgtcatca gacgctcagc catctcgcag tgcagcgttg ctccccgtta
    19441 actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg gaaaacccac tttggcgaaa
    19501 tctattgcgg gctggatcca caacaagcga cttatgagag tgaccgctgt gtttattgtg
    19561 ccgaaaaagc taactgcaac tggcattgtc cgctgcataa cgctattccg gattacatcc
    19621 gtctggtaca ggaaggaaag attattgaag cggcagaact ttgccaccag accagttcct
    19681 tacccgaaat ctgcggcagg gtatgtccac aggaccgtct ttgtgaaggt gcatgtactt
    19741 tgaaagatca ctctggcgca gtctctatcg gtaatctgga acgctacatc accgatactg
    19801 cgctggcgat gggctggcgt cccgatgtca gcaaagttgt tccccgtagc gaaaaagtgg
    19861 cggtgattgg cgctggacct gcaggattag ggtgtgctga tattctggcg cgcgcgggag
    19921 ttcaggttga tgtctttgat cgccatccag aaattggcgg tatgctgact tttggcattc
    19981 ctcctttcaa actcgataaa acggtattaa gccagcggcg agagatattc accgcaatgg
    20041 gaatcgattt ccatctcaac tgtgaaattg gccgcgatat ctctttcaac gaattaacgg
    20101 cggaatatga tgctgttttc ctcggcgtgg ggacttacgg gatgatgcga gcagatctgc
    20161 cgcatgaaga tgcgcctggt gtcattcagg cgttaccgtt cctgaccgcc catacccgcc
    20221 agctcatggg attgccggag tctgcagagt atccgctgac ggatgtggaa ggtaagcgag
    20281 ttgtggtatt gggcggtggc gatacgacaa tggattgttt gcggacttcc atccgcctta
    20341 atgctgccag cgtgacctgt gcgtatcgtc gtgatgaagt cagtatgccg ggttcgcgca
    20401 aagaggtggt caatgcccgc gaggaaggtg ttgagtttca gttcaatgtt cagccgcaat
    20461 atatcgcttg tgatgaagat ggacgcttaa cggcggtggg cctgattcgt acctctatgg
    20521 gtgagccggg gccggatggc cgccgtcgtc ctcgtccggt tgcgggttca gagtttgaat
    20581 tgcccgccga tgttctcatt atggcctttg gtttccaggc acataccatg ccgtggttgc
    20641 agggcagcgg aattaaactc gataaatggg gcctgattca aaccggcgac gtcgggtatt
    20701 tacccaccca gacgcatctg aaaaaagtct ttgctggtgg tgatgcagtt catggcgcag
    20761 atctggttgt cactgcaatg gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt
    20821 ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt aatccggctg actgtattgg
    20881 ctgccgtact tgtgaagtgg cctgtgtggt cgctcaccct tcagaacagg agttgaatgc
    20941 cgatatcttt ttaccccggc tgaaggtaca acggctggat agcatcagtg cgccggtgat
    21001 gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgt cccgtggggg cgctgacgat
    21061 gggcgagcag gtggtgcagg caaattctgc ccgttgtatt ggctgtcaga gttgcgtcag
    21121 tgcgtgcccg tttggaatga tcaccattca gtcattgccg ggggataccc ggcaacaaat
    21181 cgtgaaatgc gatctctgtg aacagcggga agaggggcca gcctgcgttg aatcttgccc
    21241 gacacaggcg ctgcagttgc tgactgaaag agaactcagg cgagtccgcc agcagcgtat
    21301 tgttgccagc agtgagaatc ctctgtgaaa gaaatctccg cgctgaaagg cgcggagaat
    21361 ctattagatt gcccaaccac ccgcgtaaaa cgcgaccagt gcggcagtaa tgatcaccgt
    21421 accaatattc agcttttgcc attcgcgcgc aaagacgcga cccaccacca gtgttacaaa
    21481 gcccagcata ataccggtaa cgatattaca agtcagaacg atgaacacgg cacacaccag
    21541 gccagccatt gcgtcaataa aatcgttgaa atccagcttc gagacgttgc ttaacatcag
    21601 caaacccacg tacatcagtg cgggagctgt ggcataaccg gggatcagaa acgataacgg
    21661 tgataaaaac agaatcatca ggaacaacac accaaccacg gttgcggtta aaccggtttt
    21721 ccccccggcg gcggttcctg ccgctgattc gatataaacc gccgcgggcg ctgcgcccac
    21781 caggccggag aatattgaac ttactgagtc actggtcagg gctttgccgc cgttgatgat
    21841 ctggttgtct ttatccaaca aattcgcctg accggcgacg gcacggatgg tgccagtggc
    21901 gtcgaacact gcggtcatca ccaatgccag tacactcgga agtacagttg gctggagtgc
    21961 gcccataata tcgaggctga aaatcagaga cttaccatct tcgccagtca ggcttggcat
    22021 cgccaccaga ccgtggtact tcaccgctgg gtcaaagatt aagccgatga tcgaaattgc
    22081 aataatcacc aacaagatcc cgccgggtac gcggcacttt tccagaccga agatgaccgc
    22141 cagccccagt aagctcatca tcaccgggaa ggaggtaaag gcaccgagcg ccactggcaa
    22201 gccttcaatc gggtttttta taaccattcc cacaccgtta gcagcaatca gcagcagaaa
    22261 caggccgata ccgatacctg taccgtgagc gatccccatc ggcaaattac gtaagatcca
    22321 ggtacgcaca ccggttacgg aaatagcggt gaagatgacg cccatcagaa agactgcgcc
    22381 cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg taaacgccgt
    22441 caaggaaata gcgcaaccaa tcgccattgg caaattggcc cataacccca tcagcaacga
    22501 gccgaagccc gcgaccagac aggtggcaac aaacacagct gcgggaggaa aacctgcttt
    22561 gcccagcatt cccggaacga cgataacgga ataaaccatg gccagaaagg tcgttaagcc
    22621 agccagtact tcctgacgaa cggtactgcc acgagcggta attttaaaat aattatccag
    22681 cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg tctccagaca taatgatgtc
    22741 ctcacgagga tgttaaagtt ttattattag ttgcgttcgt acaccagacg accatcaacg
    22801 taggtgcggt agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact
    22861 aaagaaacag agttgtcata gcgcagctgc tgtagcggag tggcagtggg ttccatcacc
    22921 acgaaatcag cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag gcccagagat
    22981 ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg atagccttgt
    23041 aattgcaata ccttgtaggc ttcgttcagc gtttgcagca tgttaaaagt tgttccggca
    23101 ccgatatccg ttcccatgcc cactttgact ttcttctgcc atgctttttt caagttgaat
    23161 aagccgctgc cgaggtaaag gttggaggtc ggacagaaag caatgctgga tttggtttcg
    23221 ctgagacgat cccactcttt ttcttcgaga tggacgcagt gagcaaagac gcagttttta
    23281 ccggtcaggc cgtactgatg gtaaacatca agataaccat catggtcagg ataaagcgat
    23341 ttcacccagg caatttcatc tttgttttca cagagatggg tatgtaccca tgtatccgga
    23401 tattcttctt tcaggcgttg cgccatcgcc atctgttcag gagatgaggt aggggcgaag
    23461 cgtggcgtaa tcgcatacaa cagacgacca tttttgtgcc agcgttcaat cagttctttg
    23521 ctttggtgat agctgctttc ggcagtgtcg agcagataat ccggtgcgtt tcggtccatc
    23581 atcactttac cggcaatcat acgcatattg atatgactgg cggcttcaaa cagcgcatca
    23641 acggattgtg gatgaacagt gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc
    23701 tgcttgatga agaacgccga catctcgcgg gcgtactcta aatcctcata acgacgttca
    23761 gtagggaagg tgtgtttatt caaccactcc agcaattgtt caccataggc ccccaccatt
    23821 tcactttgcg gataatggat atgtgtatcg ataaagcccg gtactatcag tttgccgcga
    23881 tagtcgcgca cgcgaatggt gtcaggaatt tgatgctttc cgtcttccca ttcgccaaac
    23941 cattccactt ttccctgttt aatgagtaat aaaccatcct caataaaccg cagcgcagag
    24001 gcaatttctt ccgggttatc gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct
    24061 tttaacgtgt gttctcctga catcattgac tcctttactg ttaatccatc tcttccgctg
    24121 aggtaatacc aggcagaacg ttttcctgtc ggtagccacc agggaggata atatttaata
    24181 aaatcgcagt taacccgcca gcacaaatag ggttttcaac taatacataa atagaggctg
    24241 gcaatatttt aaaaatttcg ggatcgtagg agataccaag ccctaaacct aaagaagtgg
    24301 caacaataag cgtttcacgg cgctttaaac cgttggtgat gatgatgcga atccctgcga
    24361 tggcaatcat ggaaaacatc aacgtcattg cgcctcccag aactgctgag ggaatggtcg
    24421 tgaagaagcc gccaatcatc ggaaataagc cgaggataac cagcattacc gcgatggttc
    24481 gcccgacata acgtgaagcg acgccagtca tctgaataac cccattattt tgcgcaaacg
    24541 tggttaatgg taatgaaccg acagcggagg cgataacaga aaccagacca tctgccagca
    24601 cgccgccttt cagtcgggac tgatactctt ccccctgaat gggacggcgg gaaaccattg
    24661 ccgtggcggt gatatcgccg acagcttcca gcacgctaag cagataaatc gtgccaacca
    24721 ccaggaactg atggaagcta aaactaaagc cgtatttgaa cggatgcggg atggtgatta
    24781 acggcaaatt gcgcatactg ctgaaatcca ccatgcccag gcataacgat gcgatatagc
    24841 cgacacatag cccaatggcg atccctccca tgcgtagcaa cggactgcga cagcagttaa
    24901 agccgatcac cacaatcaaa accaataaac caacgccgag atgttcgtaa ttgccgaacg
    24961 taccgctgct tttggctgca aatcctccac caaaatcaat aatgccgact ttaatcaggc
    25021 ttaagccgat catcagcacc acaatgccgc tgacggtagg cgtaataacc cgacgtaaat
    25081 agggcaggat aaaagaagag ccgacaacca gaaatgcgcc aacgaaggag acgccgagga
    25141 gcgacgacat gattaactct tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa
    25201 tcatgaccgt aacaaatgaa aaattgactg actgaattga aagtaaacca gaaccgacga
    25261 tgccgtagcg gtttacttgt aaccaggtac caataccaga ggcgatcatc gccatagaaa
    25321 caagataggc agttgtttca gcggaaagct gtaaggccgc acccacgatt aacgcggggg
    25381 ttaccatcgg aacgaaaatt gccaacagat gggtaatggc acctacgaga gcctgatgaa
    25441 agggagggcg atcctccagt tcaaatataa ggtcagaacc tgcatggttt atatcagaca
    25501 tcccttcact ccttttaaag taaatttgat catctgaatt agtaaaataa attcccacga
    25561 atttggggcg caggacgcct tgagaacggc ttatctcatt ttcgcagtca ctatttagtc
    25621 atcgttcaaa cagagaaaca ggccgccgaa gcggccattt attacttata ttttttccag
    25681 cgcggtgagt attttctccg gtgtgaaatg ccattcgcgt aaccagatgc cgcaggcatc
    25741 gtgaattgcg gtagcaatcg ccggagctgc gccatttaca ccgatttccg agatcgattt
    25801 cgccccgaat gggccgactt tatcgtcgct cggcaccagc acagcgcgga aatcacgcgg
    25861 aatgtcacca attttcggtg cgccgtaact gcgtaaatca cgcgttaacg ggtgaccttc
    25921 ggcgtcataa atgatctctt cgctcatact gtggccgata gcacgcaggg tggcaccgta
    25981 gatttgtccc aacgctaact ctggattgac cggtgtaccg cagtccagca aggcgtagaa
    26041 tttatccagg cggatttcac ccgtacgcgt gttgacggca acttcagcga agtttgcgcc
    26101 atacgggaag gcgaaatcag gtgtgatata actgccagtt cccaccagtg aaccaaagcc
    26161 ggtgccggtt tcgcctttat gggctatctc tccgaaacta acttcgcctt tcttgccgcg
    26221 aacgacgccc ggcgttgcta gttgaacatc tgccactggc tcacccaata tttgcgcgcc
    26281 gtggaacagg attttctccc gcagattttc cgctgccaaa cgcgcagcgt tacccgagaa
    26341 gcaagtaccg gacgaggcat atgcgccttt atcaaacagt gcatgatcgg tatcaccaga
    26401 gataacatgc acgtcctgcg gtgggcagtg cagcacttct gctgccagtt tggtcaccac
    26461 agtatccaga ccagtaccaa tatccgcacc accggaatga acgataaagg tgccatccga
    26521 ttccagtttg atcatgcagt tagcctgatc gatatccggg atacccgatt tctgcatgat
    26581 aatggcgacg ccgcgaccga tgtgccagtc accattttgc ggttttggtg aagaccattg
    26641 gatcatctcg cgaccctgac gcaggatctc ttccagtgcg cagctggcgg cggaaggaac
    26701 ggaggtcggc gctttacctt caccgattgc accgagaatt ttcagctctt gcccttcgtg
    26761 tacccggtta cgttcgataa tttccagttg gtcgatctgt aactgttcag ccagttccgc
    26821 taatgccatg gtgatagcga agttaccttt cggtgcgcca taaccctgat aagcaccatt
    26881 tgggcaaatg ttgctgtagt aggtggtgac ctgaaaatcg acgttatcgc atggatataa
    26941 cggcagcgac agcgccggtc cgttacacgg tacggtgagt gagtggttac cgtaaggacc
    27001 cgtgttggcg cggaaatcca tcttcactgc cgtcaggcga ccatcttttt tcgcgcccag
    27061 tttgacggtg actttcgcga cgtgacgaga ggtgttagca ataaactctt cttcacgggt
    27121 gtagcggaaa agtaccggac gcccggtcac gcaggttgcc caggcgcaca cttcttccag
    27181 caggatgtcc tgtttggaac caaaaccgcc gccaactcgc tctttaatga catgaacttt
    27241 atgctgtttc atgcccacga ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt
    27301 ggaggcgtgg atcaccagac gatcgccgtc catacgggta aagcagatat gtgtttcagt
    27361 cgggcactgc tgtgcctgcg ttgaattata ggttcgctca atgatcacat cggcatcggc
    27421 aaagcctttg tccatatcgc caatatgacc atgaatactg gcggcgatat ttttgcgtgg
    27481 gcgagaaccg atcgggaagt tgatgatcat atgctcgccg cgctgggctg catggctgtt
    27541 atcatcttcc agagtatctg gcgcaccagc aacatacacc accggttcat cgtgcacgac
    27601 aggcgcatct tccgccattg cctcgtcaat cgacattacc ggcttaagca cttcatattc
    27661 aacgtcgatg agcttcagtg cttcgagcgc aatgtcttca ctttcggcta ctaccgcagc
    27721 aacgcgatcg ccgacgtgac gcattttctt gccgaacatt cggcggtcaa gcggtgacgg
    27781 ttccggtgcg ctctgaccac ccggggtata gtaaatatcc gggcaattca ggtgagtaat
    27841 aacgtgaacg acgcccggta aggcttcagc tttgctgaca tccagatgag taatcagtgc
    27901 gtgagcgtgt gggctacgta acattttaat gacgcaggcg tcagccgtta cgcgatcttc
    27961 aacatagcag ggtttcgcct gcaccatttt cgcggcatca gttttaggat aatgcttgcc
    28021 aatgacttca aggtcgtcac ggaaagtcgg agcgatatca atggtggcct gcggattatt
    28081 tttacgtgca accgccagtt caatgacctg atagtattgc tgccagccag catcgcggct
    28141 aaacagacct gagagtgcgt cgtcgatctc ttcgcgggta ggtgcgtcga tgcgatcgag
    28201 aagatcggtg ataatcagag ccgcagctgg atcgttataa ccagactgca ccacgccaac
    28261 atcaaccatg gcctgttgaa ccagacttaa ctcattccat ttgcccagag attctgcggt
    28321 acgaatatct gccttctcta actgtgcggc gataagcaag gacgcgttaa cgatattacc
    28381 gttaaagata attgcgtcag aaccggcaaa cccaaagcca tcatcactgt tgcgtacaga
    28441 gtgcattccc atgttaaaca acagcttttg cacgttttcg cctggattaa cggttagctc
    28501 ctgaggcgcg ccatttaaag taaagtggat gatcatacgg cttcctcccc agcctgttgg
    28561 cagtcggcat acaggtcggc taccagaact cccgtgatat agcgtttata ggccacgctg
    28621 ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag cctgttccag tgcattgcct
    28681 tccagatttt gagtttcaac atcatgcaga cgcagtgctt tactggcgac gccatccagc
    28741 gcaattcgca taccgtcgtg gtctgttatt gcaacggctg ccgtcacgac ggttaaacct
    28801 gcctgagaac ggctaatttt gcgagtcgca caggtgcgat acggatcttt aatgataatt
    28861 tcggttaaca ggcgatcgca tgggcaggcc aggtagtcct cgattgacag cgtttcgccg
    28921 ttgccaaaaa ccagttcagc atccagtgcc agcaggacgg gaagcagcac tgactcttct
    28981 tgccgggcgg caatttcacc accaatggtc gactgattac gaacatggcg agagtagaca
    29041 aaaccgagag cttcccgcag tgctgcagga ataaatcgcg catcacgcag tggctgcaag
    29101 cgagacattg cgccaattcg tagtgcaccg ttatcccagt caatccagtc cagctccaga
    29161 tcctgcaagg aaatggcaat ctttttatcg gtacgggttg gtgtagcgtt gagtttgctg
    29221 cccccggcga accagacggc ttcatcctgg tagcggcgct tcagttccag cgcctgttcg
    29281 acagagtcgg gtctgaaaaa ttgttcaatc atcgtctttc cttaatttcc cctcttctct
    29341 gaaagaagag gggagctgtt atttatgcca gcgcatccat ccgacgccac atactggcag
    29401 cggcttttct ggcttgcgca taaatggaat cgcaatcgaa gttaaactga cggtcttcat
    29461 agaccatcac accattgacc atcacgctgt gaacgctacc tgagcccata ccgaaagcga
    29521 tatgcccggc gatattgtct gccagcagcg gcgtcggcga gttgtaatcg caaatggtta
    29581 aatcggcttt gtaaccggct tccagaagcc caaatttcgc gccaaagttg cggctcatca
    29641 gttcgttgcc gttagccagt gctttggcaa aactgtcagg ccacagcggg ccacccgcat
    29701 cgcgatgttt aaagaaggca aatttcatct cttcaaacat gtccgaacca atgccgtccg
    29761 ttcccaacgc cagattgcgg atgtcgctaa ggtgatggtt gtagccgaca tggttgttca
    29821 tgtttgaacg ggcgttatgc accaggaacg catcgcgctg attgagtagg acgatgtcat
    29881 ctttcgacag gtacagccca tgagcgacca gcgttttgct gtcgatgaga tcgaattgcg
    29941 caagtcgtgc cagcaggtct ttgccgtacc aatggtgact gtaggaaacg tcgtaaaggt
    30001 cttccgcaac gtgaatatgc aaaccacgac ctgtgctttt cacggcttca cgcagcatct
    30061 ccagaccggc atccggcacg gtaaacggcg cgtgagcgcc gatatgtgct tccaccagat
    30121 acggctcggt tgctgctttc ttcgcctcat caatctgacg ggtgaagcgg atgttttctt
    30181 ctacaccttc ctgcaactct ttgatgccgt tgttacggtc agtagtttca aaacaggtca
    30241 tcgcgcgcag gccaactttt aaaaatgcgt tgcgcaatgt ggagagcgac ccgtcgatat
    30301 acgccggaga ggcatggtga tcgataaccg atgtacatcc gctcttaatc gcttccaggg
    30361 aacaaatcag tccgctgtaa tagagcgact cttcatcaag agcgcgatcg agccgccacc
    30421 agagattttt cagcgttgag atgaaatccg ggcagggggc gatgtttgcc ataattccgc
    30481 gggaaagccc cgagtaaaaa tggttgtgcg agcagacaat tcccggcatc acaatccgac
    30541 catgcatctc tttgtagctg gcatcggggt agcgttgcgt cagggcatcg ccgatagcga
    30601 caatcacatc attttcgatg gcgatatcaa cgccttcctg cactttcgcc gggtgtaact
    30661 gcactgcggt gacattcttc agaatcaaca tgattacacc tccacgcggc ccagcagata
    30721 atgatgatgc tggtggacat ggctaatgat gcggcacata tcgttcagct ccggtggtac
    30781 gttgttgaac tgaccttcgc tgtcgatgtt taacacccag ctttggttat tcagacgtac
    30841 gcgtacccgg caatcttcca caaggaagcc tgggttactg ctgttatcaa agtcttgcga
    30901 caggctgaag acggtgattt tgtctttgta cggtttaccg ttccacgggc agaattgggc
    30961 gcagttgccg cattcgttgc agtaggcatc gagatgcagc gtctggaaac ggttctggaa
    31021 gccaggaacc gcaatggata cgttggcgcg gttcgggcag acatccacac acttgctgca
    31081 aacgtagtta cattcaaggc agcgtgcggc ttcctgtgcg acaaacgcgt cacgatcgtc
    31141 actgttcacc agagtgacag agatatcgcc tttacgttga tagatttccg ccggatttac
    31201 gttgttccag tatttatcgt tctggtggga acggatattt tcccgactaa ggatggtatc
    31261 ggtcgcccga cgcgcggttc cgacagcagc gacaatggag gatggtccgc gctgtacgtc
    31321 gccgatcata aagacgtcag tcagacgcgt ttcgccatta tggtcgacgt ctggccagcc
    31381 gtttttgtcc agcggcacgc ccatcgcatt cagggcttca gtatcctgct gttcaccaat
    31441 ggcggtgatc agactgtcca catgcagtgt tactgtttcg ttggtttcaa ccggacggcg
    31501 acgacctttc tcatcaggtt cgccaagcga cataacgcgc aaggttaagg tgccatcagc
    31561 atcgaaacgt tccggattat tcagaaaacg gaactccacg ccgtcgtgca acgcttcttc
    31621 atactcttcg cgccatgcgg gcatttcttg cagtgaacga cggtaaacga ccgttgcttt
    31681 ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc attgcggtgt tacccgcccc
    31741 gacaacgacc acatgtttgc ccagcttgag cgcggtgccc ttgttgtatt cacgcaggaa
    31801 agggagtgat ttccagacat tttggttgtc gcccgccagt ttcacaccgc tatttttatc
    31861 agtgccggtg gcaatcagaa catagtggaa gccctgattt tttaactgtt caacggtcaa
    31921 atcgggtgag caaccgtact caaatttcac gccgtgagcg gcaacaaaat cgatatcgtg
    31981 ctgaattaac tctgcaggaa tacggaactg aggaatgata tttttcacca cgccgcccgc
    32041 attggcttcg cgttcaaaca gcgtgaccgg atggcccgct ctggcaagga agtaacctgc
    32101 ggccagaccc gccggacctg caccaatcac ggctaccgga tggcgtgaac cggaaccggc
    32161 tggtttgtgc cagcgttgct tatattcatc ccaacctttt tccagggcga cttttttcag
    32221 ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta cagttgtatt ggcactggtg
    32281 atcgcaaata tgaccggtaa tggcgggcag ggcgttgcgt tgatagatga gttccagcgc
    32341 gtcggcatag cggtgttcgc caagcagacg gatgtattcc ggaatatctt gcttgatagc
    32401 gcaggcagta acacaggggg caacgtagca atcggtcagc ggcaggtctt cagccacttc
    32461 aatacgctct tctggcttcc agtgtttctg ggtgtattcc atggttaacg cgtctgctgc
    32521 cagtctgttc agtcgttcga cgtcaacgtg gttaagctcc caggcgtcgg agccttccag
    32581 ctcgcgcatg caggcactta agcgcagata gccgccaggt ttcagcaggt cggttgccat
    32641 agtaataggg cgaataccag tatcaaaaat atcgcggata gtcagctgac tggcaccacc
    32701 ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag agaactgctg caacgttgat
    32761 ggagagcggg aacagcgcac ggcctgacat atacatctct tcaccgggca gtgcgccttt
    32821 attgttgatg gtgccgagag tgttagtcag ttttacgcca aagccgagtg atttttcttt
    32881 tgccagtgcc atcaggcgtt ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc
    32941 aaatgactct tcttttaagc cgatgtaacc gaaaccgcag acatcgagga tctcacgaac
    33001 acgcgcgtac cccagtaagg tcgggttgag tttcacaaag gtgttgagtc ctttttcttc
    33061 cagcatgtag cggcaaatgg cttcgatttc atgcggagga cagccgtgca tggtggagag
    33121 ggtaacacct tgtaccatgc tggtggggat gcgagcgggt aaggcttgca agctttcgcg
    33181 tttttcctgc aatccgtggc gggctaaaaa tgcctcatcc tggagtagct tattaagtgc
    33241 atcgcggtat tgagcgaatt tcggatggtc agatgcgtcc atcatattgt cgatgaactg
    33301 ctgcatcggc ggttgcttaa taccttcgag gttgtaaccg acgctcatat taaagatgaa
    33361 cgatttaccg gaatcagaag gctggaacat cgcttcgaga aggtgcaggg caaaccaggc
    33421 tttgaggtat tcatcccagg ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa
    33481 gcactcgtct tcggcatcga tacagggctt ttccagctcc agacggtcaa gaatttggac
    33541 ggtttttagt tcgatgaagc gtccgccagt cagccaggaa gtgacaatat tttgcgcgag
    33601 ttgcgtgtgc gggccagcgg cagggccgac gggagtggca caggtttcac cgaagacgct
    33661 aacagtttta ctttttacgg gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg
    33721 ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat
    33781 aatatccccc ataaccctct ccttagctta tacagatatc attgagtgac cggtgttgag
    33841 aaatccggtg gtttaggggg atagagcaac aatcgcacca ctcttgccag gtttgacggg
    33901 aaatataaaa aaatgtgagg cggctcgaaa cttaatggct ttttgtgaat gaactcacag
    33961 gtgccaaatg agaattaaaa tcactcctga aaattattat tgccagccca tttatcactt
    34021 tttctctttt ctcaaaatca gtgattcctt atcattatga taaagagtgc tggagggatt
    34081 aaatatttga acgcaatcgt ttaactacgt tttctaaatt tcagtatacc ttttcttcgc
    34141 tgtaataaag tcgtccggag tatcaatatc taaaattatt tctgcatttt ctatttcgac
    34201 ggaataatgt tctcccataa ggagtgcctg acgcatattg gtgacattgg ggcgctggat
    34261 tgcctgcatc aggcatggtt ttgacactaa aatcggatgg ccggggatgc cattatggag
    34321 tggcagtatt gcgccatcat ttcgtaacga ccagattttt ctaaaaatat cgatggtgag
    34381 ggttggcata tctccgtggg tgagaaaaca atgttctgtt tgcaccgcgg gtactgcggc
    34441 ctttactgat gtcagtaaac cctgcgcata atctgggttg tggataatag tgatattgct
    34501 ctggttcgca taacgttcgt gcagttcatt accacgatag ccggtgacta aaataattcg
    34561 gctacaaaac tgcaacgcat ttttgatact tgtatcaaga attgttccct gttgccaggg
    34621 taacatcatt ttccattgcc ccattcttga tgataatcct gcggcggtaa ttatacagtc
    34681 gatggctgac atagtgattc cattatttac aggagctaat aacgcctgat aattgcgtta
    34741 cttcctgatg gattacgttg agggtaattt atcccagaga ggtccataaa gacgcgtgaa
    34801 aagtataatt gacccatcgg cattattcat tgatttaggc gcgcagaaac gtcccactgt
    34861 gatttctatt gttggtgctg gggggaaaac cagcttgctt ttttggctgg cagaactgtt
    34921 ccaggcgagt ggcaggcgtg tattaatcac tacgacgaca catatgttta tgccaacatc
    34981 tcactggccc gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt cttttatatc
    35041 ccccatttca ttttgttttc actgctggaa agcgaaccag ggaaaagtgc agggatttac
    35101 gccagaagcg attgatgcac tggtacaacg accagagtgt gacgtaattc tcattgaggc
    35161 agatggctcg cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt gcatacctaa
    35221 aagcagttgc tgcgtgattg ctgtgatggg aggacatatt ttgggtgcga aagtgagcac
    35281 agaaaatgtc catcgctggt cgcagtttgc tgatattact gggttaacac ctgatgcacc
    35341 cttgcaactg agcgatctcg ttgcgctggt tcgccaccct cagggcgcgt ttaaaaacgt
    35401 accgcaaggt tgtcggcgag tctggttcat taaccgtttt tctcaatgtg agaatgcgat
    35461 tgcgcaaagc gagctccttc aaccgctgca acaacacaac gtagaggcaa tctggctggg
    35521 cgatatacaa gaacatcctg caatcgcgcg cagatttgtg aattagcggc ccgggtattc
    35581 cgggttactg aaaggatatc cattgaggtt ggtatgaata ttttcacaga ggctgcaaaa
    35641 ctcgaagagc aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg aggctcaacc
    35701 ccccgccatt ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg tacaattggt
    35761 ggcggaatgg ttgagcggaa ggtgattgaa gagtcgcttc aggcattgca ggaacgtaag
    35821 ccgcgattat tccatggacg tatggctcgt aacggtgcgg atgctgtcgg gtcagattgt
    35881 ggaggtgcaa tgtcagtatt tatcagcgtc catggtatgc gtccacgtct ggtgttgatt
    35941 ggtgcggggc atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg atttgatatc
    36001 gccgttgccg atatttatcg cgaaagcctc aatcctgaac tattcccacc atcaaccacg
    36061 cttctccatg ctgagtcgtt tggtgcggca gtggaagcac tggatattcg ccctgataat
    36121 tttgtcctga ttgccacgaa taatcaggat cgtgaagccc tcgacaaact cattgaacaa
    36181 cccattgcat ggctggggtt gctggcaagt cgccgcaagg ttcagctttt cctacaccaa
    36241 ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc cgttggttac
    36301 aacataggtg cggaaacgcc gcaggagatc gccatcagcg tgctggcaga aatattacag
    36361 gtgaaaaata acgcgccggg tgggctgatg atgaagcctt ctcatccttc cggacaccag
    36421 ctggtggtga ttcgcggtgc gggggatatc gccagtggtg tagcgctacg tctgtatcat
    36481 gcgggtttta aagtgatcat gttggaagtg gaaaaaccga cagtgattcg ttgtaccgtg
    36541 gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac cgcccgcctg
    36601 gcaaccagct ctgcggaagc gatgaaactt accgaacgcg gatttatccc tgtgatggta
    36661 gatcccacct gttcattgct tgatgaactg aaaccgcttt gcgtggtgga cgctattctg
    36721 gcgaaacaga atttgggaac gcgggcagat atggcaccag taacaatcgc gcttgggccg
    36781 ggctttactg cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg gcactggctc
    36841 ggtcaggtga tttactcagg ttgtgcgcag gagaataccg gtgttcccgg caatattatg
    36901 gggcatacca cccgacgagt gatccgcgct cctgctgcag gcattatgcg atccaacgtg
    36961 aaattaggcg atctggtgaa agagggcgat gtcattgcct ggattggtga gcatgaaatt
    37021 aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg acggactggc cgtggtcggt
    37081 ggttttaaaa ttggtgatat cgatcctcgt ggtgaaacgg ctgatttcac cagcgtttct
    37141 gataaagccc gggcgattgg cggcggcgta cttgaggcgt taatgatgtt gatgcatcag
    37201 ggcgttaaag cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc cggatggtat
    37261 tacccatccg gcctttaaat tactgacaaa tcaccgttcc ggttttgcct tcaatccctt
    37321 ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag cgagcaaacg
    37381 aggcggcagc ttccacttta ggcagcatag aacctttcgc aaaatgacct tcttcgataa
    37441 agcgttcagc atcacttaat gacaggcgat cgagccactg ttcattctct tttccgaagt
    37501 taatggccac tttttctacc gcagtcagaa tgatcagcat atcggcatcg atcatttctg
    37561 ctaaacgggc actggcccag tctttatcga taaccgcgct ggcaccacgc agatggttac
    37621 cttcacgaat aacagggata ccaccaccgc caacggtgat caccacctgt ccggcatcta
    37681 ccagagcttt aaccgtttct ttttcaatga tatcaaccgg ttttggcgaa gcaacgacac
    37741 gacgatagcc gcgacccgca tcttctttca gagtgtaacc ctgttttgtc agtagctctg
    37801 cttcttgctc ggtaaagaac gaaccaatcg gcttggtcgg gttgaggaat gccggatcgt
    37861 tagcatcgac ttcgacctgg gtaaccagcg ttgctacagg tttattaatg ccacgagaaa
    37921 gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg cttaaggcaa
    37981 cacagacaga catcggcagc attggcgagt gcgcttcagt ttttgcggca gcttcaaagg
    38041 cctggttaat catgcccacc tgtggcccgt tgccatgagt gacgatgacc tcatgtccct
    38101 gggcaattaa atcaacaatt gcctgagaag taatttttac cgctttcatt tgtccggcca
    38161 ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat tttcttactc atttttcctc
    38221 actcatcatt cttgattgtg tatagatccc tggcagtggt tagagcacgg gagggacaaa
    38281 cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc cgtcgcagaa
    38341 aatcgtttca ccacgggata aggttctgac aatcgcgccc tgacaggtaa aaccctccca
    38401 tggcgagtag tcagcgttgt cgtggagatg gcgatgctga atttgttggc tctgacgtgg
    38461 gtctataatc accacgtcgc catcggaacc gggcgctaat aatccttttt gtggccacag
    38521 gccaaacagc ctggcgggca ttgcactggt taactcaaca aaacgctccg gtgagatacg
    38581 ccccgtcatc acgccactgg agaacagcag ttgcatgcgg ttctccacac cgggtaagcc
    38641 atttgggcag cggctgaaat cgcctttaga aatttgcagg cgttgagcca tcgagaaggt
    38701 gcagtgatcg gttgccacca cgtcaatcgc accatcgctg atgccacacc acagtttgtc
    38761 ctgctcgcgt acgttacgca gcggtgggct aagaatgaac ttcatgccat cttctgtatc
    38821 gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt tcaacccaga ctggctggtg
    38881 attcgcacgg gcaagacgca gataatccag acctaagccg ttagacaggt gcacgatata
    38941 gagcggggcg ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg cttccgcttc
    39001 gcattccaga ggtcgactca aggcatgata acgcggcgcg gttaaccctg cggcgataaa
    39061 ctccgcccgc ttgctggcga tagctgcgtc attttccggg tgcacggtgg tcagcgcgcc
    39121 ggactcatgc aagcggcgta atgcctgcaa cacttcatca tcgttgagtt tatattgata
    39181 ggttaaatag agtttaaaac tgctcagtcc ttcctcaacc atcatcggaa tttcgtcgag
    39241 gatagcgtga ttaatatgtt gaatgacacc gtgaaagctg tagtcgatga ctgctttgtg
    39301 ggcggcataa ccgcgataaa cttccagttg atggcgtaac cggcagccat ttgggccaaa
    39361 tcccatatgg tcaataatgg ttgttgtacc gccacacgca gctgcgcggg taccggtaaa
    39421 aaaatcatca caactgcgcg cgatgccgac atcaatattg aaatgcgtat ggacatccac
    39481 gccgccaggg aaaacgtaac agccagaggc atcaatttct tcgcaaggga gttgcggcga
    39541 aatatcggta cccaactggc gaacaatccc gctttcaatc agcaaatcct gcttcgcctg
    39601 tccatctgca ttaacaacaa tgccgttttt tatcaatacg cgcatagcaa actccaaaaa
    39661 ccctccggtg aaccggaggg gaataagtga agagtaatta ttcggttgcc agccagctta
    39721 atgggattgc agcgtacata gcggcacagg ttaccaggtg agatttccag gttttttcgt
    39781 ttggcgcgtg cgcttccggt tctttaccgg ggccaaaacc gatgaccgga atgccgtggc
    39841 gacccatgat ggatacgcca ttagtcgaga aggtccactt atcaacgaca ggcgctttac
    39901 caaacagccc ttcataagca ttcaccagtg ctttaacggt gaagtgatct tcttccactt
    39961 tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc agtccaggac gggcggtcgt
    40021 agttgtacat agaaacgaca gcattcgctt tttgtactgc aggcagggca cggatctcgt
    40081 ccagcgcgcc ttcccaggtt tcgccccagg tcagacgacg gtcgattgaa actgcgcagc
    40141 tgtctgcgac agcgcaacgg cttggggagg tgaagaaaat ttcagaaacg gtgagggtgc
    40201 ctttgccgag gaattcgtca taacccagac gttgggagag ttcttgtaat tcgccaagaa
    40261 tcggacccat tttgaaaatg gcgttatcac cgcgttctgg tgccgaaccg tggcagctaa
    40321 caccctgaac atcaatacga atttccatac gaccgcgttg accacggtat acctggcagt
    40381 cggttggttc ggtactgacc acaaattccg ggcgaatgcc ggattgttca ataatgtact
    40441 gccagcacag gccgtcgcag tcttcttcct gcacagtacc ggtaaccagc agggtatatt
    40501 catcttccag accgagatct ttaatgattt taccggcata taccatagac gccatgccgc
    40561 cttcctggtc ggaagtacca cgaccaccga tcagctcgtc ggtttccatg ccttcatacg
    40621 gatcgaagtc ccagtttttg atgttgccaa tgccgactgt atcgatatga gcgtccattg
    40681 ccaccagacg cgggccgtgg ccgatataac cgagaacgtt gcccattggg tcgatttcaa
    40741 ctttatcgaa gccgactttt tccatctctt ctttaatacg atgtactacg cgtttctcgt
    40801 cgcagctttc actgggaata gcaaccatat cgcgcaggaa gcgagtcatg tctgcctggt
    40861 aatcttttgc tttttcaaga atcagtttga atggaatatt cttagccatt atttttctcc
    40921 agatttttta ctccccgttt tgcagggagc agttccaata gggtgattaa ggtgctacag
    40981 cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg ccttcagtgc
    41041 tgataaccag caccacggca tctttgttca gcgccagttt ttccatcagg ctttgacgtt
    41101 gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac agcgccggat tcaccggaga
    41161 tgatgcgcgg gtcgttgccg tacggattac ccagcacgcg catacctaat gcggcaacgc
    41221 tgtcctggca ggagatgaat tgggtggcgc agttacgtag gatttcccag cccagcgggt
    41281 taggttcgcc gcaggccagg cccgccatga tggtggccat atcaccgcca acgttgacga
    41341 tgtcgccttt gacgccggag cgataaatac agtcagcttt gtcaggttca acaataatgc
    41401 tgtgcagatt ttgcgggcta tagacgtcga ccagataacc cagcacacca ccggccattg
    41461 caccgacacc tgcttgcagc agaacgtgtg tcggggttac gcccatttca cgcatttgct
    41521 cgacggcttc atccgccagt gttgcgtagc cttgcatgat ccaggttggg attttggtgt
    41581 aaccttccca tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg tgttgcatgg
    41641 tcaggcgaac ggtatcgtca tagttcatat ccgtgacgat gcactcggca ccgaggttta
    41701 gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc gcattctgtc
    41761 cgagttgctg agcggcccat gccacaccgc gcccgtggtt gccgtcggtg gtggtcgcga
    41821 aagtcatttt ttcgccaatg gcatttttca ggtgctcaaa tgatagcgtt tcgatatcaa
    41881 gatgatattt ttcacacaat aattgagcga tggcgtatgc gccgccgagc attttgaagg
    41941 cgttcagacc gaagcgtttt gattcgtcct taacgagaat tttctttaca ccaaacaggt
    42001 ttgcgagatc gtccagcgca caaagcggtg ttgggcgata accagcaatt ttctggtgga
    42061 actggcgtgc cagttttgcc tggctttgcg aaaaaagcgg tgatgtttcg ccgttgaaaa
    42121 atttgttatc ggcgatatca atctttaatg agaaaacgga catatcctat ccttttggcc
    42181 tctggaaata gagggccgac cagctgatcg gcccgagggg ttatttcacg cgttcttgcg
    42241 cttctttcag cagttgctcg agcagtgcac ctggtttggc gtatttacgg gacaggatca
    42301 tcgcggcgat gatataaggc ttccagctgg cttctttgta ggtagcgata cggtattttt
    42361 cgaatacgcc ttcagtcact tcaccttctt tgcaggatac gccgctgata tcagctggca
    42421 ggcagtgcat gtacagggct tcgccatcac gggtcagctc catcatctct tcggtacaat
    42481 gccagtcttt gtgctgtgcg ttctgtgcca gacactgttt ttccagcgct ttcagtcctt
    42541 cgtgatcgtt cgcacgcagc agttcagtac gctcttccat cactttgtaa ggtgcccagg
    42601 acttcggata aacgatgtct gcgtctttga aggcttcttc catgctggtg acctgacgga
    42661 agctaccacc ggaggcttta gcattgtttt tagcaacttc aacgacatcc gggatcaggt
    42721 cgtagccttc cggatgggcc agggtgacat ccataccgaa gcgggtcatc agaccgatga
    42781 tgccttgtgg tacagagagc ggtttgccat agcttgggga gtaggcccag gtcatggcga
    42841 tttttttacc tttcaggttt tccagtgaac caaagtgttc acgtaaccat gccagatcag
    42901 ccattgattg agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc ggacgctgcg
    42961 gcagcacacc ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc atataggcgt
    43021 tgcctgcgcc cagatacata tcgtcgcgaa taccgatagc gtcagcgcag aaggagatca
    43081 tattggcggt ttcacgcacg gtttcgccgt gagcgatttg tgatttacct tcgtcaagat
    43141 cttgttgtgc aaggccgagc aggttaagcg cggaagcata agagaagcgg gtacgggtgg
    43201 aattgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt
    43261 cagcacgcag tgcttttaac gcggcggcaa cgtccagtac ttgtttcagt tcgtctggcg
    43321 tctgttccca cgttaacaaa aaatcttttt catgcaggtg agaggtcagc gaattgatat
    43381 ccttaatcag ctcattaaca gttttcatca taaagtcccg atagtggaat gacaggcaaa
    43441 gtggtttatt tcactgtgac gtgccaaagt gattggtgtt ttcacagcta tacctcggac
    43501 taacaataag tgtgccagaa ttatccacgc ggctgcgaaa ggctaatttg acggataatg
    43561 tgtggggttg atcacatatt gatgccagtt tccggttg
//
LOCUS       DAJOTB010000021        42677 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJOTB010000021 DAJOTB010000000
VERSION     DAJOTB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.021"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            119..532
                     /locus_tag="OHN28_001155"
     CDS             119..532
                     /locus_tag="OHN28_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCQ2296590.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(577..894)
                     /gene="hspQ"
                     /locus_tag="OHN28_001156"
     CDS             complement(577..894)
                     /gene="hspQ"
                     /locus_tag="OHN28_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCQ2296591.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(952..2142)
                     /gene="rlmI"
                     /locus_tag="OHN28_001157"
     CDS             complement(952..2142)
                     /gene="rlmI"
                     /locus_tag="OHN28_001157"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCQ2296592.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2237..2515
                     /gene="yccX"
                     /locus_tag="OHN28_001158"
     CDS             2237..2515
                     /gene="yccX"
                     /locus_tag="OHN28_001158"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCQ2296593.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2512..2841)
                     /gene="tusE"
                     /locus_tag="OHN28_001159"
     CDS             complement(2512..2841)
                     /gene="tusE"
                     /locus_tag="OHN28_001159"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCQ2296594.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2933..3592)
                     /gene="yccA"
                     /locus_tag="OHN28_001160"
     CDS             complement(2933..3592)
                     /gene="yccA"
                     /locus_tag="OHN28_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCQ2296595.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3801..3888)
                     /locus_tag="OHN28_001161"
     tRNA            complement(3801..3888)
                     /locus_tag="OHN28_001161"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3852..3854),aa:Ser,seq:tga)
     gene            4316..5434
                     /gene="hyaA"
                     /locus_tag="OHN28_001162"
     CDS             4316..5434
                     /gene="hyaA"
                     /locus_tag="OHN28_001162"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCQ2296596.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLCKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5431..7224
                     /gene="hyaB"
                     /locus_tag="OHN28_001163"
     CDS             5431..7224
                     /gene="hyaB"
                     /locus_tag="OHN28_001163"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCQ2296597.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQLIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7243..7950
                     /gene="hyaC"
                     /locus_tag="OHN28_001164"
     CDS             7243..7950
                     /gene="hyaC"
                     /locus_tag="OHN28_001164"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCQ2296598.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7947..8534
                     /locus_tag="OHN28_001165"
     CDS             7947..8534
                     /locus_tag="OHN28_001165"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCQ2296599.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQG"
     gene            8531..8929
                     /gene="hyaE"
                     /locus_tag="OHN28_001166"
     CDS             8531..8929
                     /gene="hyaE"
                     /locus_tag="OHN28_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCQ2296600.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8926..9783
                     /locus_tag="OHN28_001167"
     CDS             8926..9783
                     /locus_tag="OHN28_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCQ2296601.1"
                     /translation="MSETFFHLLGPGTQLNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            9917..11461
                     /gene="appC"
                     /locus_tag="OHN28_001168"
     CDS             9917..11461
                     /gene="appC"
                     /locus_tag="OHN28_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCQ2296602.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11473..12609
                     /gene="appB"
                     /locus_tag="OHN28_001169"
     CDS             11473..12609
                     /gene="appB"
                     /locus_tag="OHN28_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2296603.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12622..12714
                     /gene="cbdX"
                     /locus_tag="OHN28_001170"
     CDS             12622..12714
                     /gene="cbdX"
                     /locus_tag="OHN28_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCQ2296604.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12794..14104
                     /gene="appA"
                     /locus_tag="OHN28_001171"
     CDS             12794..14104
                     /gene="appA"
                     /locus_tag="OHN28_001171"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCQ2296605.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEVRIPACALHQDK"
     gene            complement(14092..14304)
                     /gene="cspH"
                     /locus_tag="OHN28_001172"
     CDS             complement(14092..14304)
                     /gene="cspH"
                     /locus_tag="OHN28_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCQ2296606.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14590..14802
                     /gene="cspG"
                     /locus_tag="OHN28_001173"
     CDS             14590..14802
                     /gene="cspG"
                     /locus_tag="OHN28_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCQ2296607.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14813..15001
                     /locus_tag="OHN28_001174"
     CDS             14813..15001
                     /locus_tag="OHN28_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCQ2296608.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            14976..15206
                     /gene="ymcE"
                     /locus_tag="OHN28_001175"
     CDS             14976..15206
                     /gene="ymcE"
                     /locus_tag="OHN28_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCQ2296609.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            15196..15369
                     /locus_tag="OHN28_001176"
     CDS             15196..15369
                     /locus_tag="OHN28_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCQ2296610.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(15418..16491)
                     /locus_tag="OHN28_001177"
     CDS             complement(15418..16491)
                     /locus_tag="OHN28_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2296611.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLAFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(16574..19306)
                     /gene="torS"
                     /locus_tag="OHN28_001178"
     CDS             complement(16574..19306)
                     /gene="torS"
                     /locus_tag="OHN28_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCQ2296612.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRIDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRVPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            19389..20417
                     /gene="torT"
                     /locus_tag="OHN28_001179"
     CDS             19389..20417
                     /gene="torT"
                     /locus_tag="OHN28_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCQ2296613.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(20390..21082)
                     /gene="torR"
                     /locus_tag="OHN28_001180"
     CDS             complement(20390..21082)
                     /gene="torR"
                     /locus_tag="OHN28_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCQ2296614.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            21212..22384
                     /gene="torC"
                     /locus_tag="OHN28_001181"
     CDS             21212..22384
                     /gene="torC"
                     /locus_tag="OHN28_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCQ2296615.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            22384..24930
                     /gene="torA"
                     /locus_tag="OHN28_001182"
     CDS             22384..24930
                     /gene="torA"
                     /locus_tag="OHN28_001182"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCQ2296616.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            24927..25526
                     /gene="torD"
                     /locus_tag="OHN28_001183"
     CDS             24927..25526
                     /gene="torD"
                     /locus_tag="OHN28_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCQ2296617.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(25781..26086)
                     /gene="cbpM"
                     /locus_tag="OHN28_001184"
     CDS             complement(25781..26086)
                     /gene="cbpM"
                     /locus_tag="OHN28_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCQ2296618.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(26086..27006)
                     /gene="cbpA"
                     /locus_tag="OHN28_001185"
     CDS             complement(26086..27006)
                     /gene="cbpA"
                     /locus_tag="OHN28_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCQ2296619.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            27541..28782
                     /gene="agp"
                     /locus_tag="OHN28_001186"
     CDS             27541..28782
                     /gene="agp"
                     /locus_tag="OHN28_001186"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCQ2296620.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(28820..29047)
                     /locus_tag="OHN28_001187"
     CDS             complement(28820..29047)
                     /locus_tag="OHN28_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCQ2296621.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(29068..29664)
                     /gene="wrbA"
                     /locus_tag="OHN28_001188"
     CDS             complement(29068..29664)
                     /gene="wrbA"
                     /locus_tag="OHN28_001188"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2296622.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30037..30210
                     /locus_tag="OHN28_001189"
     CDS             30037..30210
                     /locus_tag="OHN28_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2296623.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(30293..31621)
                     /gene="rutG"
                     /locus_tag="OHN28_001190"
     CDS             complement(30293..31621)
                     /gene="rutG"
                     /locus_tag="OHN28_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCQ2296624.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(31642..32136)
                     /gene="rutF"
                     /locus_tag="OHN28_001191"
     CDS             complement(31642..32136)
                     /gene="rutF"
                     /locus_tag="OHN28_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCQ2296625.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(32147..32737)
                     /locus_tag="OHN28_001192"
     CDS             complement(32147..32737)
                     /locus_tag="OHN28_001192"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCQ2296626.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(32747..33547)
                     /gene="rutD"
                     /locus_tag="OHN28_001193"
     CDS             complement(32747..33547)
                     /gene="rutD"
                     /locus_tag="OHN28_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCQ2296627.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(33555..33941)
                     /gene="rutC"
                     /locus_tag="OHN28_001194"
     CDS             complement(33555..33941)
                     /gene="rutC"
                     /locus_tag="OHN28_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCQ2296628.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(33953..34645)
                     /gene="rutB"
                     /locus_tag="OHN28_001195"
     CDS             complement(33953..34645)
                     /gene="rutB"
                     /locus_tag="OHN28_001195"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCQ2296629.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(34645..35736)
                     /gene="rutA"
                     /locus_tag="OHN28_001196"
     CDS             complement(34645..35736)
                     /gene="rutA"
                     /locus_tag="OHN28_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCQ2296630.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            36024..36662
                     /gene="rutR"
                     /locus_tag="OHN28_001197"
     CDS             36024..36662
                     /gene="rutR"
                     /locus_tag="OHN28_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCQ2296631.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(36702..40664)
                     /gene="putA"
                     /locus_tag="OHN28_001198"
     CDS             complement(36702..40664)
                     /gene="putA"
                     /locus_tag="OHN28_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2296632.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            40719..40928
                     /locus_tag="OHN28_001199"
     CDS             40719..40928
                     /locus_tag="OHN28_001199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296633.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            41087..42595
                     /gene="putP"
                     /locus_tag="OHN28_001200"
     CDS             41087..42595
                     /gene="putP"
                     /locus_tag="OHN28_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCQ2296634.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT        10083 a        11092 c        11475 g        10027 t
ORIGIN      
        1 aatcatactt actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag
       61 ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg aggaaataat
      121 gaaagaaacc gatattgcag gcattttaac gtccactcac accattgcgc tggtgggggc
      181 aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta
      241 tcacgtcatt cctgtttcgc caaaagttgc cggcaaaaca ctgctgggcc agcaggggta
      301 tggcacgctg gcagacgttc cggaaaaagt cgatatggtc gatgttttcc gtaattcaga
      361 agcggcgtgg ggcgtggcgc aggaagctat cgccattggc gcgaaaaccc tctggatgca
      421 actgggggtg attaatgagc aagcggcagt actggcacgg gatgccgggt taaatgtggt
      481 gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggcaaaat aaaaaatacc
      541 cggaaggcga aaaccttccg ggtatgttta tgagaattag ttacgcagac gcggcgcctg
      601 gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc
      661 ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc
      721 atcctccatc accacgtgat accacggagc ggcgcgaagc tcgtcattca ccgccagctc
      781 atcaggcgac ggttcagaga gcgagtaaac cgggtcgata tccacgacca ctccgagata
      841 acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac
      901 ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac
      961 gcgacaggca aaccctttca gatatagccc ttccgggtag gtagcgatca ccggatgatc
     1021 ggcggcctga cggaactgct ctataaattg tacatcacga ccggcatcaa ttgcggcatc
     1081 cgcgatgatt ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag
     1141 aacaccgcct tcattcagca actgaatcgc cagcatgttg atgtctttat agccacggca
     1201 cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca cgataacatc
     1261 aaatttttca ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa
     1321 ctctgccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc
     1381 ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg cccatcaatg ccgatacggc
     1441 gaaaccaccg gtataggaga aacagttaag tacgcgttta ttttcgacgt agcggcgggt
     1501 agccaggcgg ctgtcacgct ggtccaggta gtagcccgtt ttgtgcccgt gctgaatatc
     1561 caccagcagt ttcatgccgt gttcttcaat cggcagcagg gcaggcggca actcgccggt
     1621 gatggggccc tgagtcagct ccatcccttc ttttttacgt accgcgacgt cgctgcgatc
     1681 gtaaatggca cattccgggt acaacgtttg cagggcgcta attaatgccg cgcgctgata
     1741 ctctgcgcca gcactcagca attgcagcac cagaaaatta ccgaaacgat cgatagtaat
     1801 acccggcagg ccatcagatt caccggcgat taaacgatag ctgtcgaggc catctttttg
     1861 cgccagccag tcacgccatt tttgtgcttg ctgcaaacgg cgggtaaaaa aagcaatgtc
     1921 gatagactca gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata
     1981 agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct
     2041 ggctttacct tccatgcggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa
     2101 tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga
     2161 tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag
     2221 gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggcgagttca
     2281 gggcgtagga ttccgctaca ccacgcagta cgaagcgaaa aaactgggac taaccgggta
     2341 cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt
     2401 ggaaaaatta atgcagtggt taaaaagtgg cggcccgcgt tctgcgcgtg tggaacgggt
     2461 gctcagcgag ccgcatcatc cctccgggga gttaacggat tttcgtatcc gttaaatgca
     2521 ttttaccggt ttaggcaggc cagcaatttt ggtggcttgc tttgccggac ctttcgggaa
     2581 cagtcggtac agatagcggc tattgccttt ctcttcgcca aatttattcg ccatcgcttt
     2641 taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg
     2701 caccacttcc cagtgttctg gcgacagagt aatcccttcg ttttctgcaa tcaccaccgc
     2761 cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc
     2821 tttaccttcg aagatcagca taattcttta ctaccttaat cagactggcg gcagtgtaac
     2881 aaaaaacaaa gccccgcata aagcgaggct cggtaagtaa ccctgggcga gattaatcgc
     2941 ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc gaaacataca
     3001 ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg ctggtttcaa
     3061 acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga tgcagagcag
     3121 gcagttgcag gaagatatta gcaaccatac caatcagcac caccacaata cccgccatca
     3181 gcataccgcc gaggaaagac atatctttgc gggtggtcag cacatacgca gagcagcaga
     3241 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
     3301 gataggtgtt cagaattggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
     3361 agataatccc ggtcggctta ttcgccgttt tgtaggtcag gaacatcaaa ccatacatac
     3421 ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
     3481 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
     3541 gggtgctaag cagcgatgta cggtcatgtg aagaactaac tatacgatcc attgagtcac
     3601 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
     3661 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggtcat
     3721 taggcagtga tataacgccc tgagaatttc gacaggcaaa agaaaaaggg gttagcattt
     3781 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     3841 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     3901 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaatag
     3961 ccaaaagtgc attgatagcg tgaaacaaca gcagattgat catttcatca ccatgaattc
     4021 cttctctatt tactcgttta gcaaccggct aaacatcccc accacccggc aaaaagaaaa
     4081 ataggtccat ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa
     4141 gtttcactac gctttattaa caatactttc tggcgacgtg cgccagtgca gaaggatgag
     4201 ctttcgtttt cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg
     4261 ccgaccatgt gcgcccggtg ttctgccggt gtcgcaagga gaagagacgt gcgatatgaa
     4321 taacgaggaa acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct
     4381 caaatattgt agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat
     4441 tgcctgggcg ctggagaata aaccgcgcat tccggtggta tggatccacg gcctggaatg
     4501 cacctgctgt accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct
     4561 ttccctgatt tccctcgatt acgacgatac tttgatggct gccgccggaa cccaggcgga
     4621 agaagtcttt gaagacatca tcgcgcaata caatggcaaa tatatcctcg cagtagaagg
     4681 taatccgccg ctgggcgagc aggggatgtt ctgtatcagc agcggacgac cgtttattga
     4741 gaaactcaag cgtgccgccg ctggagccag tgcgattatc gcctggggaa cctgcgcgtc
     4801 ctggggctgc gttcaggccg cgcgacctaa tccgacgcag gcaacgccta tcgacaaagt
     4861 catcaccgac aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag
     4921 cgccatcatt acttacatgg tgacgtttga tcgtctgccg gatgtcgaca gaatgggccg
     4981 tccactgatg ttttatggtc agcgtatcca cgataaatgc tatcgccgcg ctcacttcga
     5041 cgccggagag ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgtg
     5101 caaaatgggc tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaacga
     5161 tggcgtttcc ttcccgatcc agtcaggtca cggctgcctg ggttgtgcgg aaaatggttt
     5221 ctgggatcgc ggttcgttct acagccgcgt ggtcgatatt ccacaaatgg gtacccattc
     5281 caccgccgac accgtcggtt taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca
     5341 cgcagtcgcc agcgccgttg accagcgcag acgtcataac cagcaaccta cagaaaccga
     5401 acatcagcca ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac
     5461 accatcaaca atgccggacg ccgcctggtg gtcgacccga ttacgcgcat tgaaggccac
     5521 atgcgctgcg aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc
     5581 accatgtttc gcgggctgga gatcattctg caagggcgtg acccgcgcga tgcgtgggcg
     5641 ttcgttgaac gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc
     5701 atcgaagatg ccatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg
     5761 ctggcaacgc tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggat
     5821 tggatcgatg tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa
     5881 agcctctcct catggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg
     5941 aagaaattcg ttgaaggcgg ccagttgggg atcttccgca atggctactg ggggcacccg
     6001 cagtacaaac tgccgccaga agccaacctg atgggctttg cccactatct cgaagctctc
     6061 gatttccagc gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca
     6121 aactggattg tcggtgggat gccttgcgcc attaacattg acgaaagcgg cgcggtcggg
     6181 gcagtcaata tggaacgcct gaacctggtg cagttgatta tcacccgcac ggcggatttc
     6241 attaacaacg tgatgatccc cgacgcctta gccatcggtc agttcaacaa accgtggagc
     6301 gaaatcggca cggggctttc tgataaatgc gtcctcagtt acggcgcgtt ccctgatatc
     6361 gccaacgact ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat taacggcgac
     6421 ttcaacaatg tactgccagt ggatttagtt gatccgcagc aggtgcagga gtttgttgac
     6481 catgcctggt atcgttatcc caacgatcag gttgggcgtc atccgttcga tggcattacc
     6541 gacccgtggt acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat
     6601 gaacaggaac gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg
     6661 gggccgctgg cacgcacgtt aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg
     6721 gtcgatcgta tgatgtcggc gttgaaactg ccgctttccg gcatccagtc aacgttgggc
     6781 cgcattttgt gtcgcgcgca cgaagcgcag tgggccgcag gtaagttgca gtatttcttc
     6841 gataaactga tgaccaacct gaaaaacggc aatcttgcca ccgcttccac ggaaaaatgg
     6901 gaaccagcaa cctggccgac agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg
     6961 gcgttaggcc actgggctgc cattcgcgat ggcaagattg atctctatca gtgcgtggtg
     7021 ccgaccacct ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg
     7081 gcgctgatga acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg
     7141 cacagctttg acccgtgcct cgcctgttca acacacgtgc tgggcgacga cggtagcgag
     7201 cttatctccg tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac
     7261 aacgttgtca gtcactatgt ctttgaagca cccgtgcgga tatggcactg gctgacagtg
     7321 ttatgcatgg cggtgttgat ggtcaccggt tactttatcg gcaagccgct accttccgtc
     7381 agcggcgagg cgacatatct gttctatatg ggctacatca ggttaattca cttcagcgcc
     7441 gggatgattt ttaccgtggt tttgctgatg cggatctact gggcttttgt tggcaatcgt
     7501 tactcccgcg agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg
     7561 tatgaaatcc gctggtatct gtttctggca aaacgtccga gtgccgatat aggccataac
     7621 cccatcgccc aggcggcgat gttcggctat ttcctgatgt cagtttttat gatcatcacc
     7681 ggctttgcgc tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg
     7741 gtggaatttt tctactggac gggcggcaac tcaatggaca ttcacagctg gcatcggctg
     7801 gggatgtggc tgattggcgc atttgtgatc ggccatgtct acatggcgct gcgtgaagac
     7861 atcatgtccg acgacacggt gatctccact atggtcaacg gctaccgtag ccacaaattt
     7921 ggcaaaataa gtaacaagga gcgttcatga gcgagcaacg cgtggtggta atggggctgg
     7981 gcaacctgct gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc
     8041 attaccactg gcccgaggat gtggagattg tcgatggcgg cacccaagga ctgaacttgc
     8101 tggggtatgt cgaaagcgcc agtcatctgt tgattctcga tgccattgat tacgggctgg
     8161 aacccggaac gctacgaacg tatgccggag aacgcattcc ggcttatctc agcgcgaaga
     8221 aaatgagcct gcaccagaac agtttctccg aagtgctggc gctggcagat atccgcggac
     8281 atctgccagc acatattgcc ctcgtcggtc tgcaacccgc aatgctcgac gactacggcg
     8341 gcagcctgag cgaactggcg cgggagcaac tgcccgccgc ggaacaggcg gcgctggcgc
     8401 agctggctgc gtggggaatt gtgccgcaac cggctaatga atcgcgctgt ctcaattatg
     8461 actgtctgtc gatggaaaat tacgaaggca ttcgtttgcg ccagtaccgg atgacactgg
     8521 aggagcaggg atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg
     8581 cggctggacg ccgatcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg
     8641 cgtggtgtta ttaagcagcg atccgaaacg cacgccagag gtcagcgata atccggtaat
     8701 gattggcgaa ttattgcgcg agtttcccga ttatacatgg caggtagcga ttgctgacct
     8761 tgagcagagc gaagccatcg gcgatcgctt tggcgtcttt cgctttcccg ccacattagt
     8821 atttacctgc ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat
     8881 aaacctgatg cgcggacttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt
     8941 ttccatctgc tggggccagg aacgcaactg aacgatgaca gtttcagcat gaatccactg
     9001 ccgatcacct gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac
     9061 agcccgcagg tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg
     9121 ccgttgggcg aggtactggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt
     9181 atcaatacgc ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgatggtagt
     9241 gaaagtgaaa ttcaggaggc gatcttctgc ggattatggc gggtacgcag acgtcacggc
     9301 gaacagttgc tggaagacaa actggaggct ggctgcgcac cgctggcgtt gtggcaggcg
     9361 gcaacgcaaa acgtcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg
     9421 aatggcctac cgttggccca tgaattactg gcacatgtac gtaaccccga cgcacagccg
     9481 cacagcatta atctgacgca attacccatc agcgaagctg accggctttt tctctcacgt
     9541 ctctgtgggc cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac
     9601 gccacggggt tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta
     9661 ctggaaagtt atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg
     9721 gtcgactctg cgcagcggct tagcgaggta tgccagtggc tggcggaagg tgcaccgacg
     9781 taaaaagacg gtaagtatcg ctttcaggct tatgaatatc gcaatcggcg aatacctctg
     9841 gtcgtagagt ttcaggataa agagggagat ctaccattaa cgggttattt ttctctcttc
     9901 gcctacagga gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac
     9961 cgcgctgtat cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat
    10021 catggaaacc atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg
    10081 gggtaagctc ttcggtatca actttgctct tggcgtggct accggcctga cgatggagtt
    10141 tcagtttggt actaactggt cattctattc caactatgtg ggcgatattt ttggcgcacc
    10201 actggcgatg gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggctgttctt
    10261 cttcggctgg caacggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt
    10321 cggttcaaat ctctctgcgt tgtggatatt gaatgccaac ggttggatgc aatacccgac
    10381 tggtgcgcat tttgatatcg acaccctgcg tatggagatg accagtttca gcgagctggt
    10441 ctttaacccg gtcagccagg tgaaatttgt gcacaccgta atggcgggat acgtgaccgg
    10501 ggctatgttt attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt
    10561 cgcattacgc tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggtacgct
    10621 gcaactcgga gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc
    10681 gatggaaggg gagtggcaaa cagaacctgc acctgcaccg ttccatgtgg ttgcctggcc
    10741 ggaacaggat caagagcgta acgcctttgc catcaaaatt cccgcgctgc tagggatcct
    10801 cgccactcac tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta
    10861 ccctcgcttg caacgtggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa
    10921 tcgtgagccg catgtgttgc aggcattccg ggagctggaa ggcgacctgg gttacggcat
    10981 gttgctctcc cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt accaggcggc
    11041 gatgcgtggc gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg
    11101 ctgtggttcc ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa
    11161 aatcgaccag catcgctggg tgctgaaaat ggcgctctgg agtttgccgc tgccgtggat
    11221 tgcgattgaa gccggatggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga
    11281 catcttaccg acatactccg cgcactccgc cttaaccaca ggacaactgg ctttctcact
    11341 gatcatgatc gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa
    11401 atatgcccgt ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta
    11461 aaggagaaaa tcatgtttga ttatgaaaca ttgcgtttca tctggtggct gctgattggc
    11521 gtgatcctgg tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg
    11581 ccgctggtgg cgcgtaatga tgatgaacgt cggatagtga taaacagcgt tggcgcgcac
    11641 tgggaaggca atcaggtctg gttgatcctc gcaggtgggg cattatttgc cgcctggccc
    11701 agagtgtatg cagcggcgtt ttccggcttt tatgtggcga tgattctggt gctgtgctcg
    11761 ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt
    11821 aaaatgtggg atgccggact ggtcatcggc agtctggtgc cgccggtagt cttcggtatt
    11881 gcattcggca acttgctgct cggcgtgccg tttgctttca caccgcaatt acgcgtggaa
    11941 tatctcggca gcttctggca actgctgacg ccattccctt tattgtgtgg attgctcagt
    12001 cttgggatgg tgattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt
    12061 catctgcgtt cacagctggc gaccaaacgc gccgcactac tggtgatgct gtgctttttg
    12121 ctggcgggtt actggctgtg ggttggtatt gatggctttg tgctgctcgc ccaggatgct
    12181 aacggtcctt ccaatccatt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat
    12241 aatttcgtcg aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg
    12301 ctgacggtga tggcgattta tcgtggtcgc ccgggttggg gatttttgat ggcgtcattg
    12361 atgcaatttg gcgtgatttt cacggcaggc atcacgctgt tcccctttgt catgccgtca
    12421 agcgtgagtc cgatctccag cctgacgttg tgggacagca cttccagtca gctgacgctg
    12481 agcattatgt tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg
    12541 agctactaca aaatgtgggg gcgcatgacg acagaaactc tccgccgtaa cgaaaacgag
    12601 ctgtactaag gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg
    12661 tgttcgctct ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaagg
    12721 taagcctgat gaggcgcatt agcgtcgcat caggcaatca ataatgtcgg atatgaaaag
    12781 cggaaacata tcgatgaaag cgatcttgat cccattctta tctcttctga ttccgttaac
    12841 accgaaatct gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt
    12901 cagtcgtcac ggtgtgcgtg caccaaccaa ggccacgcaa ctgatgcagg atgtcacccc
    12961 tgacgcatgg ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct
    13021 aatcgcttat ctcggacatt accaacgcca gcgtctggtg gccgacggat tgctggcaaa
    13081 agagggctgc ccgcagccag gtcaggtcgc gattattgct gatgtcgacg aacgtacccg
    13141 taaaacaggc gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac
    13201 ccaggcagat acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca
    13261 actggataac gcgaacgtga ctgacgcgat cctcagtagg gcaggagggt caattgctga
    13321 ctttaccggg catcggcaaa cggcgtttcg cgaactggaa cgggtgctta atttcccgca
    13381 atcaaacttg tgccttaacc gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt
    13441 accatcggaa ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc
    13501 atcaatgctg acggagatat ttctcctgca acaagcacag ggaatgccgg agccggggtg
    13561 gggaaggatc accgattcac accagtggaa caccttgcta agtctacata acgcgcagtt
    13621 ttatttgctg caacgcacac cagaggttgc ccgcagtcgc gccaccccgt tattggattt
    13681 gatcatgaca gcgttggcgc cccatccacc gcaaaaacag gtgtatggtg tgacattacc
    13741 cacttcagtg ctgtttatcg ccggacacga tactaatctg gcaaatctcg gcggcgcact
    13801 ggagctcaac tggacgcttc caggtcagcc ggataacacg ccgccagggg gtgaactggt
    13861 gtttgaacgc tggcgtcggc tcagcgataa cagccagtgg attcaggttt cgctggtctt
    13921 ccagacttta cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga
    13981 ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc
    14041 cggttttacg caaatcgtga atgaagtacg cataccagcg tgcgctctcc atcaagacaa
    14101 ataaacattt gccgctgttg gtccacgcag gccatttacg cggcaaaatt ccacgcgtaa
    14161 tcctggtata agcacttctg cgtcgcgggg agtgaatgcg gaaatatgga cctggacttc
    14221 tttacgaccg tcggagggga taatgaatcc tttgccgctt ttgcgatcaa aggttttgac
    14281 aattcctgtc attttacggg acaaacaaat tccttactga aaatactgcg ctgcactata
    14341 cggggttaat aaaataaagc cagcgatatt taagaccgcc ggacggctaa aataaaattt
    14401 gcttaatctc aattatcatg cgttaatagc tgcgtcggtt tgaaagacag acagcataca
    14461 aagtagttta ctaaagcagt tctcattatc aggcattatc cccttctttt gagtctctct
    14521 cctgaacact aagtagtttc tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg
    14581 aagtaaaata tgtctaataa aatgactggt ttagtaaaat ggtttaacgc agataaaggt
    14641 tttggcttta tcactcctga tgatggcagc aaagacgttt tcgtccattt cactgccatc
    14701 cagagcaatg aattccgcac gctgaacgaa aatcagaaag ttgaattttc tattgagcag
    14761 gggcaacgtg gtcccgcggc agcgaacgtt gttacgctct aaggttgccg ttattactca
    14821 acatctccat ttccgctgtc catgttgcca tggttcacag taccgcacat cggcattcga
    14881 tgtaacggag cgaaaccctt tgggtgcaaa gtgcattttt tgtaaatcga cgatgatcac
    14941 ctttgataac gttgcgctgc aaatacgcac tgaccatgcg ccgctggatt tcacaaaata
    15001 atatcaggct ccctcgtgga gcctttttta tatctgcctt atttttcttc aacgctgtat
    15061 gtatagtaag cgataacctg ttgattattg aatctttcgg ggagatggct tataacattt
    15121 cttacctgac cagggtaccg ggaaccaaca ccttactggc gtgttgctgt cttttaagac
    15181 cagaagaggt taacagtgaa tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc
    15241 gactttatcg cgcaaaaaat cgccgagctg aacaagaata cagggaaaga agtctctgaa
    15301 attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa
    15361 ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta
    15421 gtgaccaatc attggcgatt gcggaattaa gcgttggtac aattcctccg gcaccgggct
    15481 ttgccatact cccgcataca ttgcgtaacc aatcgccgca aacataatcc ccataaccag
    15541 tagcgtcatt aaccagccag acaacgcaaa ggctttttta tttgccgcag gtttttgcag
    15601 tgaaaaggcc aatgttgagg ctaccggaca tgactctacg caagtcatac agccggtaca
    15661 ttccactgtt cgtacctgaa ttaatttatc gaccgggatc cgtgatgggc aactttttgc
    15721 gcatttgcca caatcgatac aactttcggc attgcgacga atcttaaacg gcgataatag
    15781 cgaaaccacg cccatcagcg cgccatatgg gcaaagataa cgacaccagg catggcgaat
    15841 aaacaggctg gcaatcagca aaacgatcat gctgattaat gtcgcggtcc ccatatgacg
    15901 aaagaaatcg agcattttaa cgtccatcac cacgctgtag ggcgacaaca taaaatagtg
    15961 aatcgcctga gcgggcatca ataacgcgat atagagaaaa aaactcaaca gcaaatactt
    16021 cacgccgcgc agaggaatat ccagccagcg gggaaggaca aattgccgac caaacagttt
    16081 gttaccgaga tcgccgatta attccgaaag cgtaccaacc gggcataacc atgaacaaaa
    16141 ggcctttttg agtaatagac tgatgacgat aaaagcgacc aataacagca tcgcggcggc
    16201 gtggacggac ggtaactgac ctgttgcaag gctatatttc agattcatca gaccggcaat
    16261 cggtagccag ccttcgatac cacccggtct ggtgacaaat gtcgtactac ctgccgtttc
    16321 gtaatagcgc acccaatacc agaacgtgat ggcaatataa atattaattg ccaacagtaa
    16381 taattgcgtc gctttacgcc aggtcgtggc attgcgccag tcattccacg gtaatttgcc
    16441 gcccgtcgtg cctggccgcc gttgccagcg ggttcttttt ttctctgcca tgattttgcc
    16501 agtccgttaa gttgcatacc aaatgccact attctagttg ttcttaactg gctgatattg
    16561 attcaaatcg cgttcaggtc tttcttatta accatgcttc cagagcggca acactgcgtg
    16621 taatttcttc gtgtggaagg ggggcagata atggctgctg ctccagttgt gcgcattgct
    16681 ggctggcgct acgcattccc agactcgagc aactgctttt tagctgatgt gcggcacgct
    16741 ttattttctc gttatcctga ctggcgcggg caatgtcgat ttcatcgaga agcggcaggg
    16801 catgttgttt aaataatatc agccattcgt ggatcttctc cgcccccatt aaatgagcat
    16861 cttcattgag ttgcgataca tccagtggtt gatcgttatt gacttgcagt tggagatagt
    16921 gcgccagtaa ttgaccgagc acttcacgcg gcaccggttt agggataatc ccgcgaaata
    16981 gcgaactggt acgctgtcgc agtgtttcgt caatgacatg ggcgctaaag ccaatcaaaa
    17041 ccagcgacgg atattgctgt gccagttgtc gggcaagcgt aatgccgtcg atatccggta
    17101 gatcaaaatc caccagtgcg gcagcaaacg gttcactatt ttgcagtgtc tctaaagcct
    17161 gcgcggcatt tcctacggca acgacctgcg ccccactgct gttcaacatc tcaacggtaa
    17221 tccgctgggt tagcgggtta tcttcaatta acagcaaacg taaaccgtcc agacgcaccg
    17281 cctgattgac tgtttttggc acgggtgcag tggcaacacg taacggcacg cgtaaacaaa
    17341 aacagcttcc aacctccggc gtgctggtgg cgctcaattc gccgcccatc gcctgggcca
    17401 gacggctact gatagtcagc cccagcccgg tgccgccgcg tttgccgcta acctgaacaa
    17461 atggctggaa gatttcagcc agtttcgcgg gatcaatacc gcagccgctg tcttccactt
    17521 cgaccagcca ttgttcgcca tcaatgcgac tacgcagaat aatgtgccct tcgtcggtga
    17581 aacgcagggc gttgctcaac aggttggtaa tgacctgacg aatacgccgc ggatcgccca
    17641 ttaacgcagt cggtacatcg tcggcaattt ctgttgccag gcgaatcggg cgacctttca
    17701 cccttccgct cattaattgc agggtacttt ccagcagcgg gcgcggttca aagggttcat
    17761 cgctgaccga aacattcttg ccgcctgctt cgatagccga ataatcgaga atatcgttga
    17821 ggatggtcag caacgattca ccagagtcgg taatcgcccg caaatcatcg cgctgggcgt
    17881 taagtgcggg gttatctgcc agcagctgtg cggtgccgag aataccgtac agcggtgtgc
    17941 ggatctcatg gctcatcgcc gccagaaacg ccgattttgc ctggctggct ttttctgctt
    18001 ccgcccgtgc ctgtcggtgt tctatcacca gttcctgcaa ttcagctgta cgcgctttga
    18061 cctgcgccgc cagctgttcg cggtggcgat tcagtgcatg aacactgctg cgaaacgcat
    18121 ccatcagccg cccgatggta tccagttccc gtacgccagc ggtttccggg aaaggggagt
    18181 cgatatcacc atccagcaag cgttgcagcg cctgcgtttg ttctgcaaga gggcgcgtga
    18241 ctgagcgata aaccacgcgc cagaggatca gaatcagtgc gcaaagagaa actaatccca
    18301 gcaataacag gctgtattgc ccgcgtgcac tggctttttc cagatgagcc agcccgtgct
    18361 gattacgcag ctcaatggtg tcgaccagct gactgacttc gctactaaac tgcgcgaatt
    18421 gggcgatgtt attttgggcg agagtttgca ggtgattgct gatttcactg tcctgctgat
    18481 acagcgccag caaatcgcta tattggctaa cggtagttaa cgttgttgct acctgagtac
    18541 gaacacccgg atcttcaatg cgtatttgcc gacgttgcag aattttcacc gcattattga
    18601 gctgcttttc cagcgttggc gcatttttct ggatctgttc cagccccaga ttcatcacca
    18661 tttgctgcac ccgcagggcg ctaaggcgca gttcattcat ctggttaaca tactcaagat
    18721 cgatatcaat cagccgatcg agtgcacttt ctgcggcttg acgctgatgt tgttcgatca
    18781 aatcgtaaat cccggcctgg gtcgctccgg cggaggttgc cgcattattc gcctgacctt
    18841 gcgccaggcg tgcgatttca tcggcggcgg cgactatctg ctgactgagt tgctgttgtt
    18901 gctggcgaag ttgcaaacgc tgccccacca gttccccttg ctgacgtaac gaacgggaga
    18961 tctcctgctc ctgttgttca atagcggtgg tgtcaaaacc ttgttcccgt aacgcttgca
    19021 gcaacgcatt aatcttcaga ctttgtgcgg tgagcattcg cccctgcgcc tgccacatct
    19081 tttcgttatc ggcactggtc aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc
    19141 tcaactggcg cgccatattc atggtaggaa tcaatgcctg agtgttgtct ttttccacct
    19201 ggctgataaa gcgcaggttg taccatccca ccagggtact ggtcagggtt aacagcgcca
    19261 tcagggcaaa gcccatccag agtcttcggg tcagggttaa attcacggtc ggtgcactta
    19321 aggtgaaaaa ggttgagtcg caaagcggaa tgcatctagc ataaagcctt attattgatg
    19381 aggctatcat gcgcgtactg ctatttttac ttctttccct tttcatgtta tcggcatttt
    19441 cggctgataa cctgttgcgc tggcatgatg cgcagcattt cacggtgcaa gcctctacgc
    19501 cgcttaaagc caaacgcgca tggaaactgt gcgcgcttta tcccagcctg aaagattcat
    19561 actggttatc gttgaactat ggtatgcagg aggctgctcg ccgctacggt gtggatttaa
    19621 aagtgctgga ggcaggcggc tacagccagt tggctaccca gcaagcacaa atcgaccagt
    19681 gtaaacagtg gggcgcagag gccattttgc tcggtagtag cacgacgtca tttcccgatc
    19741 tgcaaaagca ggtagcaaat ctgccggtga tcgaactggt aaatgctatt gatgctcccc
    19801 acgtgaaaag ccgcgttggt gtgccctggt ttcagatggg ctatcaacca gggcgatatc
    19861 tggtgcaatg ggcgcacggt aaaccactga acgtgctgtt gatgcccggc cccgataacg
    19921 ccgggggcag taaggagatg gtagagggtt ttcgcgcagc cattgccgga agcccggtgc
    19981 gtattgttga tattgcgctc ggtgataacg atattgaaat ccagcgtaac ctgttgcagg
    20041 agatgctgga acgccatcca gaaatcgacg tcgttgccgg aacggccatt gcggcagagg
    20101 cggcaatggg ggaagggcgt aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt
    20161 cacatcaggt gtatcgcggg ttgaagcggg gaagagtgat tatggctgcc agcgatcaaa
    20221 tggtctggca aggggaactg gcggttgagc aggccatcag gcaattacag gggcaatcgg
    20281 tgtctgataa tgtcagccca ccgattttag tcatgacgcc gaaaaatgcc gaccgcgaac
    20341 atatccgccg ctcgctgtca ccagggggat ttcgtccggt ttatttttat cagcacacat
    20401 cagcggctaa gaaataacct tcgccatgtt gtgtcaccag taaatccgca ctgagtttat
    20461 ggcgtaaacg acgaattaac acatcgacgg tgcgcaggtc agggttttcc acccgacgcg
    20521 cagagagcat acgcagcaga cgttcacggc tgagaatttc gcccggatta gtcacaaatg
    20581 ccaccagcat ttcatactct gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg
    20641 tatggcgcga cacattcagg caataaccgg caaagcgata gcagttgtcc tgagtgtgcg
    20701 gttgcgcctg tcgcgcgagg tcgattcgcc agagcaaatt tttcactcgt accaccagtt
    20761 cgcgcagctc cagcggtttg gtgacgtaat cgtctgcgcc catttccagc ccaacaatac
    20821 ggtcaatccg atcgctgcgt ccggtaacca gaataatccc taccgttgag cgttctcgca
    20881 gggcgcgggt taacatcagg ccattttcat cgggcaaatt gatatccagc agaatcaaat
    20941 ccaccggctg attctgcata atttcccgca gcccggcacc gctcgccgta acggaaacgg
    21001 tatacccctc ctgagtgaag taagattgta atcgcgcctg ggtaaccggc tcatcttcaa
    21061 caataacaat gtgatgtggc atcagagggt tttactcatt ctgttcatat ctgttcatat
    21121 tctgccgtaa gccgttcatc ctgaccagtg ccgctgttca tatttgctca ttaagatcgc
    21181 ttcactaaac cataatttta caggggctat tatgcggaaa ctctggaacg cgctacgccg
    21241 acccagtgct cgttggtcgg tactggcgct ggtcgctact gggattgtga ttggcattgc
    21301 gctgattgta ttgccacacg ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag
    21361 ttgccacagt atgcaaccgg tgtatgaaga atataaacag tcggtgcatt tccagaacgc
    21421 ctccggcgtg cgagctgaat gccatgactg tcatatcccg ccggatattc caggcatggt
    21481 gaagcgcaaa ctggaagcga gcaacgacat ttaccagacc tttattgccc actccattga
    21541 tacacctgag aaatttgaag ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat
    21601 gaaagaaaac aactcggcaa cctgccgctc ctgccataac tacgatgcga tggatcatgc
    21661 gaagcagcat cctgaagcgg cgcgtcagat gaaggtggca gcgaaagata atcaatcctg
    21721 catcgactgt cataaaggta ttgcccacca gttaccggat atgagtagtg gcttccgtaa
    21781 gcagttcgat gagctgcgcg ccagtgctaa tgatagtggt gacacgctgt actccattga
    21841 tattaagccg atttatgcgg cgaaaggcga taaagaagcg tctggttctc tgctgcctgc
    21901 ttcggaagtg aaagtcctta aacgtgacgg cgactggctg caaattgaaa tcactggctg
    21961 gacggaaagt gcaggacgtc agcgtgtact cacccaattc ccaggtaaac gcatctttgt
    22021 tgcctcgatt cgtggtgatg tgcagcagca ggtgaaaaca ctggagaaaa ccaccgttgc
    22081 cgacaccaat accgagtgga gcaagttgca ggccactgcg tggatgaaga aaggcgacat
    22141 ggtgaacgat atcaaaccga tctgggctta tgcggattcg ctgtacaacg gcacctgtaa
    22201 ccagtgccac ggcgcaccgg aaatatccca ctttgacgct aacggttgga tcggcacgct
    22261 caacggcatg attggcttta ccagcctcga taaacgtgaa gaacgcactt tgttgaaata
    22321 tctgcaaatg aatgcgtctg acaccgcagg taaagctcac ggcgataaga aggaagaaaa
    22381 ataatgaaca ataacgatct ctttcaggca tcacgtcggc gttttctggc acaactcggc
    22441 ggcttaaccg tcgccgggat gctagggccg tcattgttaa cgccgcgccg tgcgactgcg
    22501 gcgcaagcgg cgactgaggc tgtcatctcg aaagagggca ttcttaccgg gtcgcactgg
    22561 ggggctatcc gcgcgacggt gaaggatggt cgctttgtgg cagcaaaacc gttcgaactg
    22621 gataaatatc cgtcgaaaat gatcgccgga ttgccggatc atgtacacaa cgcggcgcgt
    22681 attcgttacc cgatggtacg cgtggactgg ctgcgtaagc gccatctgag cgacacctcc
    22741 cagcgcggtg ataaccgttt tgtgcgcgtg agctgggatg aagccctcga catgttctat
    22801 gaagaactgg aacgcgtaca gaaaactcac gggccgagtg ccttgctgac cgccagtggt
    22861 tggcaatcga cggggatgtt ccataacgct tcggggatgc tggcgaaagc tattgccttg
    22921 catggtaata gcgttagtac gggcggagat tactctaccg gtgctgcgca ggtgatcctg
    22981 ccgcgcgtgg ttggctcaat ggaagtgtat gaacagcaaa cctcctggcc gctggtattg
    23041 cagaacagca aaaccattgt gctgtggggc tccgatttgc tgaaaaacca gcaagcgaac
    23101 tggtggtgcc cggatcacga tgtttatgaa tattacgagc agctaaaagc gaaagtcgcc
    23161 gccggtgaaa ttgaggtcat cagcatcgat ccggttgtca catccaccca tgagtatctg
    23221 gggcgcgagc atgtgaagca cattgcggtt aacccgcaaa ctgacgtgcc gctgcaactg
    23281 gcgctggcgc atacgctgta cagtgaaaac ctgtacgaca aaaacttcct cgctaactac
    23341 tgtgtaggtt ttgagcagtt cctgccgtat ctgctgggtg agaaagacgg tcagccgaaa
    23401 gatgccgcat gggctgaaaa actgaccggc attgatgccg aaaccattcg tgggctggca
    23461 cggcagatgg cggcgaacag aacgcagatt attgctggct ggtgcgtaca gcgtatgcag
    23521 cacggtgaac agtgggcgtg gatgattgtc gttctggcgg cgatgctggg gcaaattggc
    23581 ctgccaggtg gtggttttgg ttttggctgg cactataacg gcgcaggcac gccggggcgt
    23641 aaaggcgtta ttttgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac
    23701 agtgactaca aaggctacag cagcaccatt ccgattgccc gttttatcga tgcgatcctc
    23761 gaaccgggga aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg
    23821 tgtatttttg ccggaactaa cccgttccat cgccatcagc agatcaaccg cattattgaa
    23881 ggctggcgca agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc
    23941 tttgccgata tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac
    24001 ggcaaccact ccaatcgtgg catcatcgcc atgaaacagg tcgtgccgcc gcagttcgag
    24061 gcgcgcaacg acttcgatat tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc
    24121 tttaccgaag ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa
    24181 cagggcaaag gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag
    24241 tatgtcgagt ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg
    24301 gatctcgaac cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgct
    24361 gatatgaact acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc
    24421 cacggtgggc ctggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc
    24481 cgacttcact cgcagttatg tgagtcggaa actctgcgtc agcaatatac cgtagcgggt
    24541 aaagagccag tatttattag cccgcaggat gccagcgcgc gcggtattcg tcacggtgat
    24601 gtggtacgcg tctttaacgt tcgtggtcag gtgttggctg gagcagtggt ttctgaccgt
    24661 tatgcacccg gcgtggcgcg aattcacgaa ggggcatggt acgatccgga caaaggcggc
    24721 gagccagggg cgctgtgcaa atacggtaac cccaacgtct tgaccatcga catcggtact
    24781 tcgcagctgg cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaac
    24841 ggaacagtgg agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgag
    24901 tatgttcccg cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg
    24961 tttacgcctg gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa
    25021 tcgccagtgc gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg
    25081 cggcggtgaa tgggctggaa aacagtattg ccactctgac agtacgtgac gatgcccgtc
    25141 tggaactggc cgctgacttt tgcggcctgt ttctgatgac cgacaaacaa gcggcgctgc
    25201 cgtatgcatc ggcctacaaa caggacgagc aagagattaa acgcttgtta gttgaggcgg
    25261 ggatggaaac cagcggcaat ttcaacgaac cggcagatca tctggcgatc tatctcgatt
    25321 tgttgagtca tctgcatttt tcgctgggag aggggaccgt tcctgcgcga agaatcgacg
    25381 gtttgcggca aaaaacactg acggctctgc gggaatggtt accagagttt gctgcgcgtt
    25441 gtcgacagta tgacagcttt ggtttttacg cggcactaag ccagttattg ctggtgttag
    25501 tggagtgtga ctaccaaaaa agataatgcc gtttgtaggc ctgataagac gcggcaggcg
    25561 tcgcatcagg cattagtgcg cagttgccgg atgcggcgag aacgccttat ccggcctacg
    25621 ggaacagtgc tttttgtggg cctgataaga tgcgttaagc gtcgcatcag gcattatggc
    25681 gcagttgccg gatgcggcat gaacgcctta tccggcctac gggaacagtg ctctttgtag
    25741 gcctgataag acgcgttaag cgtcgcatca ggcaacaaac tcacgggtga gccacaaacc
    25801 gggaaagccg ctggcgcagc aggcggtttt cctgcttcag gtgcgcaata tcatccatta
    25861 acgtcagcgc caccgcgatc cccggccagt ccagagccag ttcatgacgc aggcgtaccg
    25921 cgcgttgcac cacaatggcg gcatggtcgt caaataccca ggttgtctcc tggatctcac
    25981 tcggttcaac cacccccaaa ccgacaattt cattcaactc ctcttcagag atgccggtat
    26041 gcaggcaaaa ttcggtaata gtaaaagtca ccgtaacatt agccattatg ctttccccca
    26101 atctttacgt ggatcaaaag acgactgggc gtctgccagt tgctgccaca gcgcggcagt
    26161 gttttcatcc ggtttcggcg gcatcacgat tttcagtacc gcatacagat cgccggtctg
    26221 ttttttgctc accagacctt tgcctttaac gcgcaatcgt tgcccggcct ggctgccagg
    26281 cgggatagtc agcaaaatgc tttctttcag tgttggaacg gtgactttag tacccagcgc
    26341 cgcttcccac gggctaaccg gcaccactat ttccagatcc tggccgacaa tatcaaacag
    26401 cggatgtggc gcaatgtgaa tcaccagcca caggtcgcca tttggaccgc cgttttcgcc
    26461 cggcgtcccc tggcctttca ggcggatgcg ttgaccattg ccgacgcccg ccgggatctt
    26521 cacattcagc gttttcggga tttcctgttc gatcatgcca aaggcgttat aaaccggcag
    26581 gttatagctg atggtacgct tatgctcagt aagcgtttct tcgaggaaaa ccgccacttc
    26641 gatttcaata tcgtggccgc gtgtggcggg gcgttgacgg ctctggcggg catgctgacc
    26701 gaaaattgac gagaagatat cgtcaaaatc ttcggcgtta aaactctgac cgtcgccatg
    26761 atggaactga cggttaaatt gcggatcgtt gcgatgttgc cacatctgat catactcagc
    26821 gcgacgttgt tcatcactta acacttccca ggcttcggcg acctctttga agcgggcttc
    26881 ggcatccggt tctttgctga catcaggatg gtatttgcgg gccagtcgac gataggcggt
    26941 cttgattgtc ttgagatcgt ccgtcggttt cacgcccatg atggcgtaat aatcctttaa
    27001 ttccatagcg ttatctcgcg taaatcaaca caaattgaag gaacccctgt aaggtaactc
    27061 ctataagtgt agggtaatcc tcaaaatttc atatgccaac acagaatatg ttattgaaat
    27121 catcgcggag gggaggttgc ctcaaggtgg gaggaacata ttttatttac ctcactcctg
    27181 aacttttggc tcatgcttac aagttaaaca ataaggaaag gaatgcacaa ctcgactatt
    27241 taaatcgtta tgcaatttaa tgataaagac atgtgctaaa aaccattgct attagtctca
    27301 cattcttttt ttattggtaa tggtaacgcc gctgattttc tttatagcta ctgatgtaat
    27361 cattagcggc gtaaacgagg ccggggctaa tcaggcatag tttgcgtcaa aacttgcctg
    27421 tttttaaagg ggaatataga aaaacaggcg ttcaacgagc cattttgcga acctgttccc
    27481 ggaaaaatga catatttctg tcacactctt taattattga taacaaaaga ggtgtgagga
    27541 atgaacaaaa cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct
    27601 caggcacaaa ccgtaccgga aggctatcag ctacagcaag tccttatgat gagccgtcat
    27661 aacttacgtg cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa
    27721 tggcctgaat gggacgtccc cggtgggcag ctcaccacta aagggggcgt gctcgaagtg
    27781 tatatgggcc attatatgcg tgaatggctg gcagaacagg ggatggtgaa atcaggggaa
    27841 tgcccgccac cggacaccgt ttatgcctat gccaatagtc tgcaacgcac cgttgcgacc
    27901 gcacagttct tcattaccgg cgcattcccg gggtgtgata ttcctgttca tcaccaggaa
    27961 aaaatgggca ctatggaccc cacctttaac ccagtgatca ccgatgattc cgccgcattc
    28021 agtgaacagg cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc
    28081 tacaagctac tggaacaaat cgttaactat aaagattctc ctgcgtgtaa agagaagcaa
    28141 caatgttcgc ttgtagatgg caaaaatacc tttagcgcga agtatcaaca agaaccgggc
    28201 gtttccgggc cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatactac
    28261 gaaggttttc cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag
    28321 gtgttgtcga agctgaaaaa cggttaccag gacagcctgt ttacctcacc ggaagtggcg
    28381 cgcaatgttg cgaaaccgct ggtcagttat atcgacaaag ccctggtcac tgaacgcacc
    28441 agtgcaccga aaatcacggt attagttggg cacgactcca acattgcctc tctgttaacg
    28501 gcgctggatt tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc
    28561 aaaatcgttt tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa
    28621 tatgtgtacc agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct
    28681 gcgcagcgcg tgacgctgga attaagcggt tgcccgatag atgccaacgg tttttgccca
    28741 atggataagt ttgatcgcgt attgaatgaa gcggtgaaat aacagaaaac tcccccgcgt
    28801 gaagcggggg agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa
    28861 gcgaatcttt atcggtctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc
    28921 aaatcttttc cgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt
    28981 ccggcgacgt attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag
    29041 ttggcatgcg tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac
    29101 atattcccct tgataacgag caatagacag ttcttcctgg ctgggctggc gtgaaccgtc
    29161 accgcctgcg atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa
    29221 taattcctgc gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt
    29281 ccaggtggat gtgatggttt gttcctgacc gccgccagta ccggtggaac taaagacgct
    29341 cgccagtttt ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt
    29401 acgcatttga ccggacatgt taccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta
    29461 atcggccagt tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc
    29521 aaataattgc ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatccac
    29581 tttgcttgca ccctcagcaa ctgcgcgagc catcgtttca atatgtccgt acatggaata
    29641 ataaagcacc agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc
    29701 gattcgctac cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca
    29761 cttatttgat ttataacaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt
    29821 tatctcatcg cgttttttta atcataagag cggcttatgg ataattattg gagatgatat
    29881 ctattctcgc taagaagctg ttgcaggata ttaccaaacg cggatctgcc cgcgtcagtt
    29941 cactaagctt agttccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta
    30001 tgcaatatga tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc
    30061 ggcaattttg cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac
    30121 agcggaggta atttcaaaaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc
    30181 aagagcagtc acggtaaaag cgacaactag ccgggctaat caatgacgca tgcatttttg
    30241 tctgtagctc gtcaaaacgc catcaccgcc ggtagccccg gcggttttct gtttatggct
    30301 tctgatgaac aacttctggc ggtggaacgt caaccaacct tctgcttaac aacgcattga
    30361 gtaaaatcgc gccaaaggtg gctgtaccga tccctcccaa cgtaaaaccg cccagtgtga
    30421 gagcaaaatc acccgcaccc agcactaagg ttactgcgac cataatcaaa ttaccgttct
    30481 ggcttaaatc cacactgttt tgtacccaga tccgtgcgcc tgcgacggca atcagcccga
    30541 acacaacaat tgatgcacca ccaatcaccg cagccggaat agtatggatc agcgcgccaa
    30601 atttcggcga aaagcccaac agcatggcga tgacggcagc ggcaacaaac accagcgtcg
    30661 agtagacttt tgtcacggcc atcacaccga tattctcggc gtaggtggtt atgccgctac
    30721 cgccgacaga gccagaaagc atcgtcgcca gcccatcgcc tacgaatgcc cgccccatat
    30781 acgggtccat attgcgtccg gtcatcccgg cgaccgcctt gagatgacct aagttttccg
    30841 ccaccagaat caccgccacg ggcgcaatca gcatcattgc ctgaccatta aaagcaggag
    30901 tggaaaaatg tggcagaccg aaccaggcag catggctgac gagagtaaaa tcgacggctt
    30961 ttcccagccc taaaacgttg gtcatcacgc catacagcag acaggcgaca attaatccta
    31021 cgagaatcaa taatcgctgg atcatgccac gggtaaatac ggccaccagc ccaatacaca
    31081 gcaccgtcat taccgccatc cagctatcaa aggccgaagc cgatacattt ttcactgcga
    31141 taggcgctaa gttcaggcca atcgccatca ctaccgcacc cgtcacaacc ggcggcatta
    31201 gtcgttcaat ccagcgcgta ccgattttca tcaccaccag gccaatgacg gtataaacca
    31261 gcccacaggc gataatcccg cccagcgcga tgctgatgtt cggattaatt ccctgaccat
    31321 taaagcccgt cgcggcaatc accacgccga caaaagccgc gctggaaccg agataactgg
    31381 gcacgcgccc gccggtaata aagaaaaaca gtaacgtgcc gatccccgac attaaaatgg
    31441 aaagattggg atccagcccc atcagaatcg gcattaacac cgtcgcgcca aacatcgcca
    31501 ccgcgtgttg aacgcccatt actgccgttt gagcaaacgg caatcgttcg tccggcgcga
    31561 ccacgccgct ctctgtagag gtcgatttta actgccagtg aggaaaaccg aacattgcca
    31621 tcagctgtct ccttaaggag gttaacaagc agggcgcatc agcgcgtgat aactgcgatc
    31681 gaaccacacc agcccgtagg gtgtggcgtg acgatgaatc gcttcgatgg cgcaaaacaa
    31741 aatgtcgtgg gtgccgacgc tcaccacctg gctgatacgg cagtcaaacg aaaccagtgc
    31801 cttttccagt tgcgggcatc cggtcacacc agtctgccag cgggcggcgg caaagcggtg
    31861 ttccatgggc gttttgccgc caaaaaggtt tgaaagcggc tcctgcccgg cgctaagtgt
    31921 atttacacac agcgttcgat tttcattgaa taccggccag acggacgccc cacgattcag
    31981 gcacaccagt aatgtgggcg gcgtgtcggt cacgctgcag acggcgctgg cggtgaaccc
    32041 ggcgcgcccg gctggaccgt ccgtagtgat gatattgacc gccgcgccca tacaggacat
    32101 cgcatcgcga aaggtttgtt gatcgacaat gttcattgtt tgctccttac aacagcccgc
    32161 aggcttcttc aaaggacaga cgtggcaggc gcgcaaaaag tttgctggaa tcgccatagc
    32221 cgatattaat cagcagatcg ctcttcagcg tgctgcccgc aaaaaaggcg tcgtcaacgt
    32281 attgacggtc aaagcccgac atcgggccgg tatccagccc cagcgcacgg caggcgacga
    32341 tcagataggc cgcctgcatg gaactgttgc gaaacgctgt ttcttcggca agttgtggac
    32401 tggaggtaaa ccaactgcgg gcatcgccgt gaggaaacag ctgtggtaac cgttcataaa
    32461 attcactgtc ccaggcgacg atagcggtga cgggcgcggt cagggttttt tgcagattgc
    32521 cgctggaaag cgccgggcgc agacgttctt ttccttctgc agtgcggata aacacgatcc
    32581 gggccggaga acagttagct gatgtcggcc cccatttcat cagggcataa atctcccgta
    32641 acgtctcatc gctgacgggg ggctcccgcc agccgttgtg agtgcgggca tcggtgaaca
    32701 gggtgctaag cgcacctggg ctaacggctt cgttcatagc aattccttac agagcggctt
    32761 cacggtgatg taacaggctg gcaagcccgt tgagtaacag agcattaaac gtttcgggat
    32821 cggtcacgtt gcaggcgtgt ccgccatagc gcatcaccat tttctggcta tcgggcaggg
    32881 cggcatgaag ttcactggaa catgctgttg gcaccagcag atcgtcactg gcgcagatga
    32941 tttgcaccgg gcagcggata cgatccgcat ggtgactaaa gtcagcgcgt ttgagggcgt
    33001 taagtcgacg cagtaaatta tttttgccct gaaaatgcgc cagtgccagc gcatcttctg
    33061 cctcaaggcg aggtgcgcgg gccgccatcc agtcggcggg atagaggaac aacggctgcg
    33121 cttccaccca tgcctgcgcg ccgccgctat gtagtaactg ttcgcgaacc tgaaaacagc
    33181 ggcgcgtatg ggtgtttatt cgtagccagc cgttaacgct gaccagcaca gttaccgacg
    33241 cgggataatc cagcgccagc tgcattccca ccagcgcacc gagcgcatgg ccgaccaccg
    33301 cgtaacgctc aatccctgcg gctaccagcg cctgatgcag ttccgctgcc atctgggcga
    33361 tactgtaatc ttctgccagc gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga
    33421 ctacctgata ctcctgatcc agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc
    33481 caagacccga aatcaacacc actacgggcg catcagcata agggggaggt gagagtgaaa
    33541 gtttcatcgc ggcctcactt ggcgatatgc gcaattgtgg cgatttccac cagcgcgtca
    33601 ggttttacca gtccgcactg aatgcagaat cgcgccggtt tgtcacccgg gaaaaactcg
    33661 gcgtagattt cgttaatcgc ggcgtaattt ttccagtcgg taataaagat gctgttgaaa
    33721 gtcacatccg ccatcgtgcc acccgccgtc tcgatgacct tgcggatggt ttccagaacg
    33781 tggcgggttt gcgcctttgg gtcatcggca aacagcacgt tgttatgttg atcaaaagcc
    33841 agcgtaccgg agacatacac cacgccatca gccagcgtgc cgggaacgaa gggggccagc
    33901 ggtgcgctgc tgccagcagg aataattacg gattttggca tcgttaaact ccttaagcga
    33961 tacgagcaaa ggacgtggaa gaaagcgcgt cgcagaacgt ttcgacgtcg ctgacccagc
    34021 caaaaaaggt ttcgatattg aacaacgcag ctttctgtgc aaattccggc cccgcctggt
    34081 gtgttgcgtc ttccagcacc acgccgaaat actccagaaa aaagccgtcg cgtagcgtcg
    34141 attcgacgca gacgttggta gcgataccgg tgaaaaccag atggcgtatt ccacggctgc
    34201 gcaaaatgct gtccagcggc gtattgaaga aaccgctgta gcgcggcttc ggcagcacaa
    34261 tatcgccagg ctgcggcacc agttcatcca ccagttgata atcccaggag cctttcgcca
    34321 gcaatttccc ctgcagctgc ggctgcttac gcatggtttt cagggcgttc gatttatgaa
    34381 aattcggtga gccggggccg ccagcctcga catactgttc atcccagcca ttttgaaacc
    34441 agatgatcag catccctgcc gctcgcgctg cggtcacggc ggtttgaatg ttggcaatga
    34501 ccgggcgagt ggttgagaca tcaaacccgg cgagatctaa gtagccgcct ggcgtggcat
    34561 aagcgttttg catatccacc acgatcagcg cactttgctg cggatcgaag gtaatggctt
    34621 ccggtcgagc ggttaaggtc gtcatcatgc cacctcctga gtcagcgcag ggagatgggc
    34681 gcggcactgc atcagtggtt gaatgcgctc gccgaaggtt tcgattcccg acagaaaatc
    34741 atcgaaggtc aacagcacgc cttcggcacc aggcacgttt gcgacttcat ctaacatgcg
    34801 cgcgacactg gcgtaagaac cgactaacgt ccccatattg atgtttaccg ccgaagtggg
    34861 atcggccatc tgacggacgt tggtgtcagt acctgagcgg gtatctttct gactttgttc
    34921 ggttagccag cttaacgcct cttcatccgc gcccgctttg tagtgttccc attttgcacg
    34981 agcggcatcg tcggtttcgt cggcaatcac cataaacaac acataagagc caacgtcgcg
    35041 tccggtttgc tctgcggcct gtttcatccg cgcagcggtc ggggcgaaag ccgtgggtgt
    35101 atttacgcct ttgccgaaac agaagttaaa atcggcatac cgggcggaga acgccatgcc
    35161 agcgtcgctt tgcccggcgc agatcacttt catggggaca ctcggttgcg gactgacgcg
    35221 acaatcatcc atagtgaaaa aatcgccttt aaaatcgctt ttccccgagc cccacaggtc
    35281 gcgcagcacc tgaacatatt cggtgagata gtcgtaacga cgggagaaat agtcatcgcc
    35341 aggccagatc cccatctgct catactcggg cttctgccag ccagtcacga ggttgacgcc
    35401 aaaacgcccg ccagagatgg agtcgatggt tgcggccata cgggcgacga ttgccggagg
    35461 taacgttaag gtggcagctg tcgcgtatat ctgaatgcgc gaggtcacgg ctgccagccc
    35521 cgccatcaag gtgaacgact caaggttgtg atcccagaac tcagttttgc cgccaaagcc
    35581 acgcagtttg atcatcgaca gggcgaaatc gaaatggtag tgctccgctt tttgcacaat
    35641 ggctttattc agttcaaatg tcggcatgta ctgcggcgcg tgggtcgaaa tgagccagcc
    35701 gttgttgcca ataggtacga atacgccaat tttcatcatc aacctctctt cgtctcgtaa
    35761 agtgaaagtc agacggggcg ctgcatcctg catatccttt tcagccgcgt aatggcttgt
    35821 ttgcaaagcg gatgccagtt tttaaaaagt gaatgttatt aatgtgttaa tgttaagttg
    35881 tctaaaatgc ctgaaaaaaa gtggactaaa cggtcaaaac agttgcacat aaaacatgca
    35941 ttcgtgcgcg atgagagtgc agaaggtcga ggccgggcgg gggttttgct atcctgttgc
    36001 caatctacaa gaggggagag cgcatgacgc aaggcgcagt gaaaacaacg ggtaaacgtt
    36061 cgcgcgcagt gagcgcgaag aaaaaagcga ttcttagcgc agcactggac actttttcac
    36121 aattcggttt tcacggcaca aggctggagc agatcgcgga gttggcgggt gtttcaaaaa
    36181 ccaatctgct gtattacttt ccgtcaaaag aggcgctgta tatagccgta ctgcggcaga
    36241 ttctcgatat ctggctggca ccgttaaaag cgtttcgaga agatttcgcc ccgctggcgg
    36301 cgatcaaaga gtacatccgt ctgaagctgg aagtttcccg cgattatccg caggcttcgc
    36361 gcctgttctg tatggagatg ctggcaggtg caccgctgtt aatggatgaa ctgacgggcg
    36421 atttgaaggc attaattgat gagaaatcgg cgctgattgc cggttgggtt aaaagcggca
    36481 aactcgcgcc gattgatccg cagcatttga tttttatgat ttgggcttcc actcaacatt
    36541 acgccgattt tgcccctcag gtggaggcgg tgacaggcgc gacgttgcgc gatgaggtat
    36601 ttttcaatca aacggttgaa aacgtgcagc ggattattat tgaggggatt cgaccacgct
    36661 aatggtgccg gaggaaggtg taacatcctc cggctacctg tttaaccgat cgtcattaaa
    36721 ctggcgttac cgccagcggc agcggtattc acactcagcg aacgctcgat atacagccgt
    36781 tccagaagga tattgctttc gccacgggca aaaccctgca ccgaaacaat tgcaccatcc
    36841 cgcgcggcaa cggcttcaca caatgcgcga agctgatccg aatcaccgtg gaagatcacc
    36901 gcatcaaatg gttgagcggt tatattttcc gctttcgcca gttgaatacg ttcgctgact
    36961 gccgatggca atgccttcac taactgacga tgcagcgcat tatccggcca cagcacctgg
    37021 ctacccaccg ccagcacggc ggcgagctga gtcagcgcat cctgttcatc atccgcaata
    37081 cacaacacgc gctcacgcgg cagcagcgtc caggtgttgc gttcacccgt cggccctggc
    37141 aacaatcgtt gtgttcctgc ctgcgccaac tcgccatatt gcgtacataa cgcctgcaat
    37201 tctggacggt ttgccgccca ttcccgcagt gcatttagcg gctgagtcaa tgcggctttc
    37261 aactgcgcat cgaccggata ctccgcatcc tgacgcgcga gcgtcaccgc cagcgcactt
    37321 tccgggcgat tcgccagcag acggtagaga tagagcggac cgcctgcttt cggcccggtg
    37381 ccggacaatc cttcgccgcc gaacggttgc accccaacca ctgcgcccac catattacgg
    37441 ttaacgtaca gattaccgac atgcgccgag ccagtgacct gggcgatggt ttcatcaatg
    37501 cgcgtatgga cgccaagcgt cagaccataa ccggaagcgt taatctgctc gatcagctct
    37561 gggagctggt tacggttgta acgcaccaca tgcaacaccg gaccaaagac ctctttttgt
    37621 aattcggcaa agtcatccag ttcgatcagc gtcggagcga caaaggtgcc gctttgccat
    37681 tcacgggcat cttcgctgtt ttcccgcacc gcctggaaca cctgacggcc tttactacgc
    37741 atggtctgaa tatgacgctc gatattggct ttcgcttcgc tatcaatcac cggaccgata
    37801 tcggtggtca ggcgacccgg attacccatc cggcattcgg ccattgcgcc gcgcagcatt
    37861 ttcagcgtgt ggtcggcaat ctcatcttgc aggcacagca cgcgcagtgc cgaacaacgc
    37921 tgacctgcac tgtcgaacgc cgaggccagc acatctatta cgacttgttc ggtcagtgct
    37981 gaagaatcga caatcatcgc gttcatgccg ccggtttcag cgatgagcgg aatagggcga
    38041 ccctgagcgt ccaggcggct ggcgatattg cgctgcagta acgtagcgac ttcggttgaa
    38101 ccggtaaaca tcaccccgcg cacgcgatca tcacccgtca gttgcgcgcc cacggtttca
    38161 ccccgacctg gcagcaattg caccacgcct ggcggtacac ccgcttccag caaaatggcg
    38221 atcccttgcg cggcaatcag cggcgtttgt tctgccggtt ttgccagcac gctgttacct
    38281 gccgccagtg cggcagcgat ctgtccggtg aaaatagcca gcgggaagtt ccacggactg
    38341 atacatacca caggccctaa tggacggtgg gtttcgttag cgaaatcatc ccgcacctgt
    38401 ccggcatagt agtggagaaa atcgaccgct tcgcgcactt cggcaatggc gttactgaag
    38461 gtttttccgg cttcacgcac cagaatacca atcagttgct gcatctggct ttccattagc
    38521 acggcagcgc gatgcaaaat tgccgcgcgt tcagccggag gagtggcaaa ccagattggc
    38581 gcgttattaa ccgcactttc cagcgcctgt tctacttcac gcggcgtggc ttcacgcaca
    38641 tagcccacaa tatctttcgg ttccgcaggg ttaataacgg gcgacatctc acctgccgct
    38701 accggttgtt ccagcattgg caaggactgc catttttgca gtgcactatt gagcagggct
    38761 gaagagagcg aggccagacg gtgttcgtta gcgagatcca gccctgccga gttgtcgcgc
    38821 ccgtgaccgt aaagatcgcg cggcagggga attttcggat gcggtaatcc agtttgccct
    38881 tcctgttgcg ccagtttttc tacagcagtg actggatcgg cgaccagttc atccagtggc
    38941 aaagaggtgt cggcaatacg gttaacaaac gaggtgttag caccgttttc cagcaggcgg
    39001 cgcaccagat atgccagcag cgtttcatgt gtaccgaccg gagcataaat acgacacgga
    39061 cggttaagtt tgccgtcggc aactttcccg gtgacctgct catacagtgg ctcgcccata
    39121 ccatgcaggc actggaactc gtactgaccc gggtagtagt tttgacccgc cagttgataa
    39181 attgccgcca gcgtatgggc gttgtgcgtc gcgaactgcg ggtagattag atttggcacc
    39241 gccagcagtt ttttcgcaca ggcaagataa gaaacgtcgg tatacacctt gcgggtataa
    39301 accggataac cttcaaggcc gtccatctgt gcacgcttga tttcactatc ccagtacgcg
    39361 cctttcacca ggcgaatcat cagacggcga cggctgcggg tggcgagatc aatcaggtaa
    39421 tcgatcacca acgggcagcg tttttgataa gcctgaataa caaaaccgat gccgttccag
    39481 cctgccagtt caggctcgaa acagagtttt tccagcagat cgagggagat ctccaggcga
    39541 tcggcctctt cggcatcgat gttaatacca atatcgtact gacgcgccag cagggtgagt
    39601 gatttcagac gcgggtaaag ctcttccatt acccggtcat actgggcgcg gctataacgc
    39661 ggatgtagcg ccgacagctt gattgaaatg cccggacctt catagatgcc acgaccgtta
    39721 gacgctttac caatggcgtg aatcgcctgc tgataggaaa ccatatacgc ctgtgcgtct
    39781 gcggcagtca gtgcggcttc gcccagcata tcgtaagagt aacggaaacc tttctcttcc
    39841 agtttgcggg cattggctaa cgcttccgcg atggtttcgc ccgtgacgaa ctgctcgccc
    39901 atcaggcgca tcgccatatc cacacctttg cggatcagcg gttcaccgct cttaccgata
    39961 atgcggttca gcgagcggga gaggctggct tcgttatggg tggaaaccag tttgccggta
    40021 aacagcagcc cccaggtagc ggcattaaca aacagggacg ggctacgacc aatgtgtgac
    40081 tgccagttac cgttactgat tttgtcgcga attaacgcgt cacgggtggc tttgtcggga
    40141 atacgcaaca acgcttccgc cagacacatc agcgccacgc cttcctgcga tgacaacgaa
    40201 aactcctgca ataacccctg aaccatacct gcgcgaccac tggcgttttt ttgattacgc
    40261 agtttatcgg ccagttgata cgccagtttg tgcgcctgtt cagcaactgg ctgcggcagg
    40321 cgagcttgtt ccagcagcat agaaaccgct tcggtttccg ggcggcgata ggccgcggtg
    40381 atcgcggcgc gggaaaccga ctggggcaat atttgttcgg caaagtcgag gaaaggctgg
    40441 tgtggttcct ctgccggagt cggtgcttca tcgctctcat tggccgcgcc agaaagcagc
    40501 gcaggtagct ccgggagagt atcgctgttt tccagttgtt caagataaga aaaaatcgcc
    40561 tgtttaatta accagtgtgg tgtgcgatcg atacgtgtcg cagcagactt aatacgctca
    40621 cgcgtcgcgt cgtccagctt aacccccatg gtggtggttc ccatgccatt actcctgttg
    40681 ttctgaaagg tgcaacttaa cgttatcgtg aaatatccat gatgttgcaa ctttgtgcaa
    40741 ccatgttaaa tgtgacctgc gtagcaagct taaaaatgaa tgaaatgtta ataaacgaaa
    40801 tcgatatgac agggattaaa aaaataactc agactttttc tctgcggcag ttaacatttt
    40861 tgaaaggtgc aaccgcaaaa aatgtgagag agtgcaacct gatgaaaaat agtgtcgctg
    40921 agcactaaaa tttaatgtaa atggtgtgtt aaatcgattg tgaataacca gcgcttccgg
    40981 caggatacgg tcgccctggt aaaacataaa ctctgttacc ccgttccggt ggcagatata
    41041 acggcaagtt tcgacattgc cgataataat tttttggaga ctttagatgg ctattagcac
    41101 accgatgttg gtgacatttt gtgtctatat ctttggcatg atattgattg ggtttatcgc
    41161 ctggcgatca acgaaaaact tcgatgacta tattctgggc ggtcgtagtc ttgggccatt
    41221 cgtgacggca ttatcggcgg gtgcgtcgga tatgagcggc tggctgttaa tggggttgcc
    41281 tggcgctgtt tttctttccg ggatttccga aagctggatc gccattggcc tgacattagg
    41341 cgcgtggatc aactggaagc tggtggccgg gcggttgcgt gtgcataccg aatacaacaa
    41401 taacgcctta acattgccgg attatttcac cgggcgcttt gaagataaaa gccgcatttt
    41461 gcgcattatc tccgcgctgg ttattttgct gttcttcacc atttattgcg cttcgggcat
    41521 tgtggcaggc gcgcgtctgt ttgaaagtac ctttggcatg agctacgaaa cggctctgtg
    41581 ggcgggggct gcggcgacga tcctttacac ctttattggc ggtttcctcg cggtgagctg
    41641 gactgacact gtacaggcca gtctgatgat ttttgccctg atcctgacgc cggttatcgt
    41701 gatcattagc gtcggtggct ttggcgactc gctggaagtg attaaacaaa agagcataga
    41761 aaacgtggat atgctcaaag ggttgaattt tgtcgccatt atctcgctga tgggttgggg
    41821 gctgggttac ttcgggcagc cgcacatcct ggcgcgtttt atggcggcag attctcacca
    41881 cagcattgtc catgcgcgtc gtattagtat gacctggatg atcctctgcc tggcaggggc
    41941 ggtggctgtt ggtttctttg gtatcgctta ctttaacggg cacccggcgg tagcaggtgc
    42001 ggtaaaccag aacgctgaac gcgtgtttat tgaactggcg caaattctgt ttaacccgtg
    42061 gattgccggg attttgctgt cggcgattct ggcggcggta atgtcaacgt taagttgcca
    42121 gctgctggta tgttccagtg caattaccga agatctatac aaagcatttc tgcgtaaaca
    42181 ggccagccag aaagagctgg tgtgggtagg gcgtgtgatg gtgctggtgg tggcgctggt
    42241 ggcgattgcg ctggcggcaa acccggaaaa ccgcgtgctg ggcttagtga gctacgcgtg
    42301 ggcaggcttt ggcgcggcgt ttggtccggt ggtgctgttc tcggtgatgt ggtcacgcat
    42361 gacgcgcaac ggtgcgctgg cggggatgat catcggtgcg ctgacggtta tcgtctggaa
    42421 acagttcggc tggctgggtc tgtacgaaat tattccgggc tttatcttcg gcagtatcgg
    42481 gattgtagtg tttagtttgc tgggtaaagc gccgtcagcg gcgatgcaaa aacgctttgc
    42541 cgaggccgat gcgcactatc attcggctcc gccgtcacgg ttgcaggaag ggtaagggaa
    42601 ggcgttttac cgaaaatttc acgtcataac aggaagcagg cagagggcaa tcagtgattg
    42661 ccctctgcgt tttttct
//
LOCUS       DAJOTB010000022        41412 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJOTB010000022 DAJOTB010000000
VERSION     DAJOTB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.022"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..751
                     /locus_tag="OHN28_001201"
     CDS             <1..751
                     /locus_tag="OHN28_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCQ2296635.1"
                     /translation="HNNVLTLNQNLSSVLTNKNNIISGYLIQPNTTINDINNWLNANL
                     NRRVALLYDNPSLSNSDIQLLATNQLISYHVVLNNKMLPNQCALLPLSKLIIISDNFR
                     KLAKNADYNGPELFTHAHQFVGQNYLGFGDYTITGQVLDLGGGQPSAVAAHLVFKNLQ
                     ANTVWIRHFVSSNTQRGSSNVTAKFLDVSDQITNLVPQHPTQFGSNIGLNYYYYNSQP
                     TVRHFPGLPKNKQYQITHHICFMLDLIAGRI"
     gene            complement(731..1906)
                     /locus_tag="OHN28_001202"
     CDS             complement(731..1906)
                     /locus_tag="OHN28_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2296636.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTAGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARTKRDEARSMLANGINPSEARKADKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPEFVNA
                     LDNYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAINILKKLQEITGNYSLVFPGRNDVSKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYIKDRQVMMEWYASLLNTTCY"
     gene            complement(2155..2355)
                     /gene="torI"
                     /locus_tag="OHN28_001203"
     CDS             complement(2155..2355)
                     /gene="torI"
                     /locus_tag="OHN28_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCQ2296637.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2412..2579)
                     /locus_tag="OHN28_001204"
     CDS             complement(2412..2579)
                     /locus_tag="OHN28_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296638.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(2698..2940)
                     /locus_tag="OHN28_001205"
     CDS             complement(2698..2940)
                     /locus_tag="OHN28_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCQ2296639.1"
                     /translation="MFSLIQRGQIYTDSAGYPVKIIRSTDHSVFFKRMDGYPGRVCIR
                     KFNNLFEHIDHREYHQILAETEQENHLKKLRAMQRR"
     gene            complement(3002..3667)
                     /locus_tag="OHN28_001206"
     CDS             complement(3002..3667)
                     /locus_tag="OHN28_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
                     /protein_id="HCQ2296640.1"
                     /translation="MTTITKERIELFIKNQLENGLTRGEQMELARIALASLERELIRH
                     EHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWADMHFLLWDAQRRAGISDA
                     EITAAMENKLKINMERQWPEPKDGEPRLHIKEPGNSPVIPDGWIRCSERMPEMGERQC
                     YVLAADFKNNYPPNIPNTQVGVYGDWFNDGKPTWDDGDGEDLYLKEVTHWMPLPEAPL
                     EVK"
     gene            complement(3664..3873)
                     /locus_tag="OHN28_001207"
     CDS             complement(3664..3873)
                     /locus_tag="OHN28_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296641.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRTFAPGHKVLRINT"
     gene            complement(4053..4796)
                     /locus_tag="OHN28_001208"
     CDS             complement(4053..4796)
                     /locus_tag="OHN28_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCQ2296642.1"
                     /translation="MSKIDYQALRAKAEKATCGEWSLEYGEGRFDGDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKSRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKASSNFEECAAMAERIEELEKATT
                     GQDANINSQQERVAQSANRHVYHYNAVSNVSGITMSGIAQSPFRIKSQSDLEEFKSAL
                     SKVCGFHATAVTSLSYLGREEEEQNDEKP"
     gene            complement(4783..5037)
                     /locus_tag="OHN28_001209"
     CDS             complement(4783..5037)
                     /locus_tag="OHN28_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296643.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWRLVMAEGGEIAWELDDGSRMVLKAEKQEATSEQD"
     gene            complement(5034..5201)
                     /locus_tag="OHN28_001210"
     CDS             complement(5034..5201)
                     /locus_tag="OHN28_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCQ2296644.1"
                     /translation="MRGLAYNPSILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGNQ"
     gene            complement(5212..5508)
                     /locus_tag="OHN28_001211"
     CDS             complement(5212..5508)
                     /locus_tag="OHN28_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCQ2296645.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLENIKNNFDAFLALPPETKAER
                     LYRRDIQLALKDEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISND"
     gene            complement(5532..6119)
                     /locus_tag="OHN28_001212"
     CDS             complement(5532..6119)
                     /locus_tag="OHN28_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCQ2296646.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAHIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCAGIGQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKAEGKQPETI
                     ANMVSSLKDKDNRSKNVSQNHADDYGYSQNDYPPF"
     gene            complement(6116..6796)
                     /locus_tag="OHN28_001213"
     CDS             complement(6116..6796)
                     /locus_tag="OHN28_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCQ2296647.1"
                     /translation="MGTATLILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKDW
                     LAWDARAKKGTVVTTDKWDVIVAVIKRAHEYGKRIVIVDDFQYVMSNEFMRRSEEKSF
                     DKFTEIGRHAWEVIKAAQDAPDDLRVYFLAHTEETPMGRVKMKTIGKMLDEKITVEGM
                     FTIVLRTLTRDDQFFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGINNV
                     HQIKENAA"
     gene            complement(6805..6993)
                     /locus_tag="OHN28_001214"
     CDS             complement(6805..6993)
                     /locus_tag="OHN28_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296648.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            complement(6990..7103)
                     /gene="kil"
                     /locus_tag="OHN28_001215"
     CDS             complement(6990..7103)
                     /gene="kil"
                     /locus_tag="OHN28_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCQ2296649.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAIEAYRKWRSK"
     gene            complement(7096..7236)
                     /locus_tag="OHN28_001216"
     CDS             complement(7096..7236)
                     /locus_tag="OHN28_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCQ2296650.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            complement(7308..7676)
                     /locus_tag="OHN28_001217"
     CDS             complement(7308..7676)
                     /locus_tag="OHN28_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HCQ2296651.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLVAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            complement(7974..8264)
                     /locus_tag="OHN28_001218"
     CDS             complement(7974..8264)
                     /locus_tag="OHN28_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296652.1"
                     /translation="MEFKGTEGKWEIMMDGDEIKIIQADSLENGTGWRSSIAICDEVQ
                     CIEDANLIAAAPDLLNALKAMLNKAYKQNWNDHYPDEVSKAQSAISKALGEE"
     gene            complement(8302..8625)
                     /locus_tag="OHN28_001219"
     CDS             complement(8302..8625)
                     /locus_tag="OHN28_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296653.1"
                     /translation="MEALVVERSEDGYWTHPEYANLFGDREVISVDEFRSFCKQHGIE
                     SSIVEMENDNNQTVIDAYFEDGNQNISGWEPSMPDGEGWFVGSIHDTEDGPICVWFRN
                     VDKDE"
     gene            complement(8634..8960)
                     /locus_tag="OHN28_001220"
     CDS             complement(8634..8960)
                     /locus_tag="OHN28_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCQ2296654.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNKRTWYRKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(9482..10339)
                     /locus_tag="OHN28_001221"
     CDS             complement(9482..10339)
                     /locus_tag="OHN28_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCQ2296655.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTALRLGNLVDNKRWQLTTFSLDALMKGTKKWDELTNDDFYYDIKQ
                     KQVDIKLGMDITTLAYEKLVDVIVLVAGDSDFVPAAKHARIKGIDFILDPLRQNVTPS
                     LSEHIDGVQSYSLISGLADALHVEPDPAPDWWEDRKKGKPRGKNNSGKRRYGNTQAES
                     AKKHQRNKR"
     gene            complement(10467..11180)
                     /locus_tag="OHN28_001222"
     CDS             complement(10467..11180)
                     /locus_tag="OHN28_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCQ2296656.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            11281..11481
                     /locus_tag="OHN28_001223"
     CDS             11281..11481
                     /locus_tag="OHN28_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCQ2296657.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            11600..11893
                     /locus_tag="OHN28_001224"
     CDS             11600..11893
                     /locus_tag="OHN28_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCQ2296658.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            11926..12072
                     /locus_tag="OHN28_001225"
     CDS             11926..12072
                     /locus_tag="OHN28_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCQ2296659.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            12065..12925
                     /locus_tag="OHN28_001226"
     CDS             12065..12925
                     /locus_tag="OHN28_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCQ2296660.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSPDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            13033..14913
                     /locus_tag="OHN28_001227"
     CDS             13033..14913
                     /locus_tag="OHN28_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCQ2296661.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEIVKRYEVVSGKVWNGERELDALVI
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            14991..15431
                     /locus_tag="OHN28_001228"
     CDS             14991..15431
                     /locus_tag="OHN28_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCQ2296662.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            15428..15955
                     /locus_tag="OHN28_001229"
     CDS             15428..15955
                     /locus_tag="OHN28_001229"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCQ2296663.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            15952..16128
                     /locus_tag="OHN28_001230"
     CDS             15952..16128
                     /locus_tag="OHN28_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCQ2296664.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            16131..16514
                     /locus_tag="OHN28_001231"
     CDS             16131..16514
                     /locus_tag="OHN28_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HCQ2296665.1"
                     /translation="MDYSQLSDFEINVAVFEAIHNGSPDYKEGENGAMVFISFEGDIV
                     NGDAVEVEVERGSFNPCANPADAWPIITENNISIILDNPSMPCATDNARDLFDDAGPN
                     VGVAYDNPLRAAMIVFLMIQDANNA"
     gene            16507..16692
                     /locus_tag="OHN28_001232"
     CDS             16507..16692
                     /locus_tag="OHN28_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCQ2296666.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCERCIYECL
                     NMVEHNEKYKQHRRIKK"
     gene            17070..17222
                     /locus_tag="OHN28_001233"
     CDS             17070..17222
                     /locus_tag="OHN28_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ninF"
                     /protein_id="HCQ2296667.1"
                     /translation="MEYLLASLTLLQKGRGQKKNWVFMKTMADHNCAELMSDPNSSMH
                     EEEDDG"
     gene            17215..17826
                     /locus_tag="OHN28_001234"
     CDS             17215..17826
                     /locus_tag="OHN28_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCQ2296668.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRAELIRRIG
                     IEQVEAIESDHKRHRWTTEECKAIKAEYQQKLKDLRDSRSEAA"
     gene            17991..18248
                     /locus_tag="OHN28_001235"
     CDS             17991..18248
                     /locus_tag="OHN28_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296669.1"
                     /translation="MTDIRREVCEEYLDALITVELSVRFAHLEDRKVNATIRATVTEL
                     LKRIRDKKIRAIFAGLSHQPFPDGALKMMRRQLDSLVGEPV"
     gene            18245..18427
                     /locus_tag="OHN28_001236"
     CDS             18245..18427
                     /locus_tag="OHN28_001236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296670.1"
                     /translation="MSTVTNIQQVKWQRHADEQSLKSLDAKIKDAENALVVLLHHPRE
                     LVNRLDLNKLDGPEVA"
     gene            18427..18945
                     /locus_tag="OHN28_001237"
     CDS             18427..18945
                     /locus_tag="OHN28_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCQ2296671.1"
                     /translation="MGVRELNLTKDQHDWLNGWLELWGAWVYSGRLEKRMSSVIAQFM
                     ERVEPSRVMTRPMCNDDDGMLISQVVDSVMRIDTKAFGILLSYYSHGSSKRAIASYYH
                     ATAKPRKMAGRSGEGWRRPSLATCRNEIDDILKASLFVLYQPMQDAFKSRKRVEKIKH
                     VAPKMLDNLLAI"
     gene            19486..19809
                     /locus_tag="OHN28_001238"
     CDS             19486..19809
                     /locus_tag="OHN28_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCQ2296672.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            19793..20269
                     /locus_tag="OHN28_001239"
     CDS             19793..20269
                     /locus_tag="OHN28_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCQ2296673.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20266..20703
                     /locus_tag="OHN28_001240"
     CDS             20266..20703
                     /locus_tag="OHN28_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCQ2296674.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKDQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAASCAKSKTGASGM
                     GDGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLR"
     gene            20691..20843
                     /locus_tag="OHN28_001241"
     CDS             20691..20843
                     /locus_tag="OHN28_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296675.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLDIEHLA
                     RGKGLR"
     gene            20925..21404
                     /locus_tag="OHN28_001242"
     CDS             20925..21404
                     /locus_tag="OHN28_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCQ2296676.1"
                     /translation="MTQHIGVKLINAFPMTRQAYNDFRGWQLPAGENGEDEGYLVEYL
                     DGGKPNTDRFDGYVSWSPKEVFERAYRPVSGLSFGLAMEALKQGKSLQRAGWNGKDQF
                     VYLMKGEKLASALGYGFGEYVGEPTFNDTLVLKNSQNRLATWVPSIGDLMAEDWQII"
     gene            21708..21950
                     /locus_tag="OHN28_001243"
     CDS             21708..21950
                     /locus_tag="OHN28_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCQ2296677.1"
                     /translation="MAEITPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            21953..22393
                     /locus_tag="OHN28_001244"
     CDS             21953..22393
                     /locus_tag="OHN28_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCQ2296678.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPKFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            22390..23805
                     /locus_tag="OHN28_001245"
     CDS             22390..23805
                     /locus_tag="OHN28_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCQ2296679.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYTAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            23807..26005
                     /locus_tag="OHN28_001246"
     CDS             23807..26005
                     /locus_tag="OHN28_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCQ2296680.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISIKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLKN
                     KNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVS
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            26096..26989
                     /locus_tag="OHN28_001247"
     CDS             26096..26989
                     /locus_tag="OHN28_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCQ2296681.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPAITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            27008..28261
                     /locus_tag="OHN28_001248"
     CDS             27008..28261
                     /locus_tag="OHN28_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCQ2296682.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGAATSITLAAAPAHKVEAKDANDMPVDNRQGTITVSAEGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYANVDANAASSAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            28303..28491
                     /locus_tag="OHN28_001249"
     CDS             28303..28491
                     /locus_tag="OHN28_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296683.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKHKVAKAEEDGENEG"
     gene            28472..28933
                     /locus_tag="OHN28_001250"
     CDS             28472..28933
                     /locus_tag="OHN28_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HCQ2296684.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMS
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            28943..30361
                     /locus_tag="OHN28_001251"
     CDS             28943..30361
                     /locus_tag="OHN28_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCQ2296685.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNISGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVSGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVIGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            30361..31062
                     /locus_tag="OHN28_001252"
     CDS             30361..31062
                     /locus_tag="OHN28_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2296686.1"
                     /translation="MVDSSLNDPVVVQATRLDASILPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDARITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIDIQAHLVTVDQRLDNVENDVSGIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVVGARQTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            31062..31517
                     /locus_tag="OHN28_001253"
     CDS             31062..31517
                     /locus_tag="OHN28_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCQ2296687.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            31520..32212
                     /locus_tag="OHN28_001254"
     CDS             31520..32212
                     /locus_tag="OHN28_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCQ2296688.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQFNTIMNNLKPFTPLADKYVGSLENLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQQWLSGQMNNYNNLANIGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANRPSALQQGVSGAASGALLGGGIASALELSTPWGAG
                     IGAGLGLLGSLF"
     gene            32223..33497
                     /locus_tag="OHN28_001255"
     CDS             32223..33497
                     /locus_tag="OHN28_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016049818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCQ2296689.1"
                     /translation="MATWQQGINSGGFLAGIGAQNENAPKASDINATLGLIRENNELA
                     RSGANNVGLTALRGLAGVADIYKQEQQQKAISAFNKVHADAWASGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNEQQRNDMGDLAMRANVALSQGPEAYSKFITDNKDRLNRVGANAD
                     WMIQTGIQNPEQLSHMLTTMTLGAVGPDKMLDYQDKMVGRQQEQQRINETIRNNDMTN
                     ARGWASNNIAQQNVNLRRMELEDKKYDRLIANETNALKLAELHDKRLQNQQAMEQAKR
                     DKADAYNSGMDNLSRTIETATKVLNSPGFTGYFGTNLNPLSSRFIPGTEAADTETLVD
                     TLKSQGFLSGIQQMKGMGALSNAEGQKVMDAIGSLSPNQSEKSARAAIKTIIKTTEMA
                     QKRMQQKYGKDIQPSQQQLSDDDLINKYLGGQ"
     gene            33497..35407
                     /locus_tag="OHN28_001256"
     CDS             33497..35407
                     /locus_tag="OHN28_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCQ2296690.1"
                     /translation="MAYSREQLMTALRNADAAGDTEGARRIAQILSSSNQPTQNQKQP
                     VEQQDGFMSDLGEAVKETGRGLVQAGVNVANIPASVADAVTSAAAWAGGKLGIGDGTY
                     QPAPRVTTKGLEQAFGLQQGALTPQTTEGRVFAEALPYLATVGVGGASTQAPTLAGRI
                     TQGAARLLAENAVGSLAANSEKNDAGKLATDIGVGMLTGGAVNTVAKGLERGITAFKG
                     DIAPEVAKKIATSESMGVTPMTSDVIPPKNAFTRGLTQGAEGALLGTGSKRAEQYAKR
                     SQLVKKQLEKYGEYSPSVVVDDLYGSLKSRKDSAGSVIEDITAKMGDTPVDTSKSIKV
                     IDNVLTRANRLGKVANKDLIRGLSDLREELAKPDIDFGLLRELRSALRESIQGDAMVF
                     PNSAKAATDAVERAMGADLRNNAARYLGAGEAARYVKANSDYSNVFNKVLNKRIANNL
                     NKAKKEFTPELINSVVFSRKPSDIKRIWPALSEDGKNAMRAAYISKIAEKAGDSPTKF
                     LTELNKLKSQSDGQIYNTIFSGRHMKELDALHEVLQQTARSDAANVVTQTGQSQANRI
                     RTIGATATLGVSLGLEAGFGAIMRLYESKAARNALLRLANTKAGTPAYERALSKAANA
                     IRPLLATEATQQ"
     gene            35644..36009
                     /locus_tag="OHN28_001257"
     CDS             35644..36009
                     /locus_tag="OHN28_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296691.1"
                     /translation="MKKVNIGNVPKMLVPLFESGTIVFCRDFPEWQRLHQKLGVDVHD
                     SDTNGASHTMSSENGVLHVIGVFNGKLSTIAHECAHMAFDICSRVGVDVEPGRANETY
                     CYLMSRLVEFCERHIKKPE"
     gene            36273..36458
                     /locus_tag="OHN28_001258"
     CDS             36273..36458
                     /locus_tag="OHN28_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296692.1"
                     /translation="MTIEERLNNIELNQTLLDQRLSDLELKDLDAQISEAEAKLSSLN
                     HRKKQIRNRITQGRGSC"
     gene            complement(36433..36642)
                     /locus_tag="OHN28_001259"
     CDS             complement(36433..36642)
                     /locus_tag="OHN28_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296693.1"
                     /translation="MNKKQFIKSKTSSKEELEKELNSLKYALCLVYSRLPMEDKNAIY
                     NEMISSLDFNDRDLASHLNSFRVPE"
     gene            complement(36639..36890)
                     /locus_tag="OHN28_001260"
     CDS             complement(36639..36890)
                     /locus_tag="OHN28_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCQ2296694.1"
                     /translation="MSDRKYKNPQVNLRLPVEIKERLIELAEANSRSLNAEMVAALEA
                     WTEKNKHIQALDLATIASRLIDLEHDVETLKIMYGKDAK"
     gene            36996..37136
                     /locus_tag="OHN28_001261"
     CDS             36996..37136
                     /locus_tag="OHN28_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCQ2296695.1"
                     /translation="MKVKTLRMPEKLEKILEEKAKEECRSFSAEVIKRVLDSLKREGV
                     TV"
     gene            37370..38293
                     /locus_tag="OHN28_001262"
     CDS             37370..38293
                     /locus_tag="OHN28_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2296696.1"
                     /translation="MKSIATAVSTINVPFHGAELYVVNHNGEPYTPMKPIVEGMGMDW
                     ASQFTKLKQRFAKGIVEIAIPSVGGVQTMICLALRKLNGWLQTISPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVVNPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEFIGKQEELPAPKLDINF
                     PISWFSENAPYAIIRQQCGDTVALDRSALVECSPAYKLIDILTKAGYDVSAVRAELKA
                     LRHLMAEQSWALKEIASFAAIRDRACHSIKL"
     gene            38416..41361
                     /locus_tag="OHN28_001263"
     CDS             38416..41361
                     /locus_tag="OHN28_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HCQ2296697.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHIPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQLRQQLEDPDGANKYPKLQIARWRDSYDVRGWGAIGDGVHDDTSALSE
                     LLSVATGGEKIDGRGLTFKVSTLPDVSRFKNARFLFERIPGQPLFYASEDFIQGELFK
                     ITDTPWYNAWTQDKTFVYDNVIYAPFMAGDRHGVNNLHVAWVRSGDDGKTWTTPEWLT
                     DLHENYPTVNYHCMSMGVVRNRLFAVIETRTVSGNKLQVAELWDRPMSRSLRVYGGIT
                     KAANQQVAYIRITDHGLFAGDFVNFSNSGVTGVTGNMTVTTVIDKNTFTVTTQNTQDV
                     DQNNEGRYWSFGTSFHSSPWRKTSLGTIPSFVDGSTPVTEIHSFATISDNSFAVGYHN
                     GDIGPRELGILYFSDAFGSPGSFVRRRIPAEYEANASEPCVKYYDGILYLTTRGTLST
                     QPGSSLHRSSDLGTSWNSLRFPNNVHHSNLPFAKVGDELIIFGSERAFGEWEGGEPDN
                     RYAGNYPRTFMTRVNVNEWSLDNVEWVNVTDQIYQGGIVNSAVGVGSVCIKDNWLYYI
                     FGGEDFLNPWSIGDNNRKYPYVHDGHPADLYCFRVKIKQEEFVSRDFVYGATPNRTLP
                     TFMSTSGVRTVPVPVDFTDDVAVQSLTVHAGTSGQVRAEVKLEGNYAIIAKKVPSDDV
                     TAQRLIVSGGETTSSADGAMITLHGSGSSTPRRAVYNALEHLFENGDVKPYLDNVNAL
                     GGPGNRFSIVYLGSNPVVTSDGTLKTEPVSPDETLLDAWGDVRYIAYKWLNAVAIKGE
                     EGARIHHGVIAQQLRDVLISHGLMEEESTTCRYAFLCYDDYPAVYDDVITGQREMPLT
                     DNDGSIIVDEDDNPVMVMEDIIERVEITPAGSRWGVRPDLLFYIEAAWQRREIERIKA
                     RLDLIEGKH"
BASE COUNT        11434 a         9311 c        10053 g        10614 t
ORIGIN      
        1 acacaataac gtcttaactt taaatcaaaa tttatcaagt gtattaacaa ataaaaataa
       61 tatcatctct ggatatctta ttcagcccaa tacaacgatt aatgatatta ataattggtt
      121 gaatgctaac ttaaatagac gagttgcact tctatatgat aatccatcat tatccaactc
      181 tgatattcag ttgttggcga caaatcaatt gattagttat catgttgttt taaataataa
      241 aatgcttcct aatcagtgcg ctcttcttcc tttgtcgaag ttaattatta ttagcgataa
      301 ctttagaaag ttagcaaaaa atgctgatta taatgggccg gaacttttca cgcacgcaca
      361 tcaattcgtg ggacaaaatt acttaggatt tggcgattat acaattactg gccaggtact
      421 agatcttgga ggtggtcaac cttctgctgt tgccgcacat ttggtgttca agaatttaca
      481 agcgaataca gtttggattc gtcattttgt atcaagcaac acgcaacgag gaagttcaaa
      541 tgttactgca aaatttttag atgtttcaga tcaaattaca aatcttgttc cccaacatcc
      601 aacccagttt ggcagcaaca ttggtcttaa ctattattat tacaatagtc aaccaactgt
      661 aaggcatttc cctgggttgc caaaaaacaa acagtatcaa attactcacc atatttgttt
      721 catgctggat ttaatagcag gtcgtattta ataagctagc gtaccattcc atcattactt
      781 gtcgatcttt aatatattgc gcatgattgt aagtacctcg aatagagttc ttatcaacat
      841 gcgcaagctg catttcaatc caggcacttt caaacccatg ctcatgcaag atggtgctca
      901 tggtatgcct gaaaccgtga cccgttaacc gaccgtgata acccagtagt tttatcactt
      961 tgttgatgct ggcttcgctc attggtttac tgacgtcatt ccggccaggg aaaacgaggc
     1021 tgtaattccc tgttatttct tgcagctttt tcaggatatt gatcgcctga gttgataacg
     1081 gaaccagatg ggggcggcgt ttcttcatcc gttctttggg gatctcccat aaggcattat
     1141 ctaaatcaaa ctcagcccat tccgctgcac gtagttcaat tgttcgcacg cctgttagca
     1201 tcaacagctg cgtggcgtat ttggtaacta aactgccctg atagttatcc agagcattaa
     1261 cgaactcggg caactcgctt tcagtcagga acgggaagtg ttgggtttta ggtttgttga
     1321 gtgcgatagc taaatcgggt gcaaaattat actttgctct accagtggca acagcataac
     1381 gaagcacttc accacagcgg cggcggatct tactggtttg ctctagcgca cctcttttct
     1441 ctattttttg caggacggtt agcagttcta atggttcaat ctgctcaatt gggcgctggc
     1501 cgataaaagg aaaaatatcc ttttccatac aattgagaac ctcttttgca taaccctctg
     1561 accaggtggc ttttttcgat gagtgccatt ctctggccac agactcaaag ctgttctcat
     1621 gcgcgaactg caaagcaatc ttatctgctt ttctggcttc acttgggttt atcccgttgg
     1681 ctaacatcga acgcgcttca tcacgtttgg tacgtgcctg tgccaacgac acatcgccat
     1741 atacgccaaa ggaaatcatc ttaggcttac ctgcaaagcg atagcgaaaa cgccagcctt
     1801 tactgcccgc tgtgtcgatc aggagtgata gccccatacc atcgttaagc gtatagggtt
     1861 tgtccttcgg ttttgctctt tttatttgga tgtcagatag cagcatgtgt atagaaaaaa
     1921 gatcgaactc agttatacac aatgttatac gcaaagatgt atagattcca ttagaaataa
     1981 tggtactttg ctggacgagt ataaggagat gatatcagtt aaaacagtga gttatggaat
     2041 taattgaact tagtgagaag tgttcatggt gtcccctgca gacatctact tgaagcggca
     2101 ggggattgat tggaatggtg ttttttagat gtgaaaaata ttttacccgc tattttaccc
     2161 attggcgcgg cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc
     2221 tcgcccgtgg ataactttgg ctttaggcag gtccccggac ttaatccggt catagatgaa
     2281 ggttttacca aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggctg
     2341 tagttcgtgt tgcatgagtg ctatcccaaa tagggaatcg aacctgcaaa tcaggcaata
     2401 aaaaaccgcc atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttacg
     2461 tctgcatgtg ctatctgcgc ccatatcatc cagtggtcat agcagtcatt gatgttctct
     2521 gcttcgataa ctctgttgaa tggttctcca ttccattcac ctgtgactcg gaagtgcatt
     2581 tatcatctcc ataaaacaaa actcgccgta gcgagttcag ataaaagaaa ttcccgcgag
     2641 tgcgaggatt gttattcatt gccgatattc acctttatcg cgaacacctt taccggttta
     2701 tctcctttgc atggcacgta attttttcag atggttctcc tgctctgttt cagccaggat
     2761 ctggtgatat tctctgtgat caatgtgttc gaataaatta ttgaattttc tgatgcacac
     2821 ccttccagga tagccatcca tcctcttgaa gaacactgag tgatcagtac tacgaatgat
     2881 ttttactgga tagccggcgc tatcggtgta tatctgacca cgttgaatca gagagaacat
     2941 tcgtttatcc ccagataaaa atatagcaat accaattaca taaaatactt caggccaagc
     3001 atcatttcac ctccagcggc gcttctggta gcggcatcca gtgggtaacc tccttgagat
     3061 acaggtcttc gccatcaccg tcatcccaag tgggcttgcc atcattaaac cagtcgccat
     3121 atacgccgac ctgagtgttg gggatgtttg gcgggtagtt gtttttaaag tcagcagcta
     3181 acacatagca ttgtcgctct cccatttctg gcattcgctc actacatctt atccaaccat
     3241 ccggaattac cggagagttg ccgggttctt taatgtgcaa gcgaggctca ccatcttttg
     3301 gttcaggcca ctggcgttcc atgttgatct tcaatttatt ttccatagca gcggtaattt
     3361 cagcatcgct gatgccagca cggcgctgtg catcccacaa caggaaatgc atatcagccc
     3421 actcgctgag atcgtctggt tcggctgcgg cttccagagc ctcttttgag aggtgtttca
     3481 gtggaccaat ggggccaacg cagccaaatg tggagtcaga ccatttggca tgctcgtggc
     3541 gaatcagttc gcgttccagt gatgccagtg caattcgtgc cagttccatt tgttcgccac
     3601 gagtaagccc gttttcaagc tgatttttaa tgaacaattc aatacgttct ttggtaatag
     3661 tggtcatgtg ttaatcctca aaactttatg cccgggcgca aaagtacgtg ttttgtcttt
     3721 gcttattcgc cagccatcct tgcgcgcctc ttttgcacag ccagcccatg acgtaccgat
     3781 atattcaccg aagtctggta ctggatatac accttccgta cactggcggc agtcacaata
     3841 gagatgcatg gtgtaacttg cggcaatagc catatcagtc tcctttagtg cgcaagtggt
     3901 ttttccagcg gttttgcgcc gcgcttctgc tccgccagac accgtaaaaa cgaatagagg
     3961 aacaccccct aaaacccaga tatctatagt aaataacaag ccgatttgag atacggatac
     4021 gctgcccggg ctttgctatc agcatctttg ccttacggtt tttcatcgtt ttgctcttct
     4081 tcctcacgcc caagatatga aagagatgtg actgctgttg catggaaacc acaaaccttg
     4141 ctaagtgcgc ttttaaactc ttcaaggtcg gattgcgatt tgattctgaa aggtgactgc
     4201 gcaatcccgg acatggtaat gccgctaaca ttgctcactg cgttgtagtg atacacgtgc
     4261 ctattagctg attgagccac gcgttcctgt tggctgttta tattggcgtc ctgccctgtg
     4321 gtggccttct ccaactcttc gatacgttca gccatcgcag cacactcttc aaagttgctt
     4381 gatgcttttc gctcccattc ggcgcattgt tttccaagct ctgcaatcag cttgtctttg
     4441 ccttcaagct caacacgcag tttccctacc gttagcgcaa tatcctcgtt ctcctggtcg
     4501 cgggatttga tgtattgctg gttcctttcc agctcatcca gcagcgccaa gacggtagcc
     4561 ggattggctg cggcgatgaa ttcagcattt gcctgctgtt ccatttggaa atcttcatcg
     4621 aaaccgcttt ctggatgcgc tccttcaatt ctgcaaatgg gaagatatcc aacaacttca
     4681 cgatgaatta gcgcatcatc accatcaaat cggccctctc catattcgag cgaccactcg
     4741 ccacacgttg ctttttctgc cttagcacgc agtgcctgat aatcaatctt gctcactggt
     4801 tgcctcctgc ttttctgcct tcaacaccat gcgagaacca tcatccagct cccacgcgat
     4861 ctcaccacct tcagccatga ccagtcgcca caccaattga gcagcctcat tggtaacatc
     4921 acgacctgga tcattgccaa cgcgcatacg tccaccttca acatcgcgca tttttgccag
     4981 catgatagtt tttgatagcg gtgaaaaacc aagctgtagt cgtgctgaat tactcactgg
     5041 ttgcctcctt tgcgccacat cgcattcaga tatttgtttt gattcactga cggaaaagaa
     5101 tttctcttaa gcaattcctc tctcgatggc attggcttta cgcgttggcg aataatcatt
     5161 tctgccggaa gaatgctggg attgtatgca agtcctctca tggtaaattc ctcagtcatt
     5221 actgatagcg ccatagcgtg agcggtaatt acgcaggcgc gggtcgatat attcagggaa
     5281 tttgtctatt gtcgcttttc gcaacggtct cattgctgtt tcgtttgttc ggtccttctc
     5341 atcttttaac gcgagttgta tatcgcgtcg gtacaaccgt tctgcttttg tttctggtgg
     5401 cagagcaaga aacgcgtcga aattgttttt gatattttcc agcacctccg ccttggagct
     5461 accggagcag ttgcgcgggt catccgcacc atacagaggc gctggcataa tgggagcctt
     5521 attttcagta atcagaaagg agggtaatcg ttctggctgt aaccataatc atctgcatga
     5581 ttctggctta cgtttttaga gcgattgtct ttatctttga ggctggaaac catgttggcg
     5641 atagtttctg gttgcttgcc ttctgccttt tctttaaggg tttgacctgt ttgtgcaata
     5701 aacgggatgc gtatttccat ctggtagctg tctgcgccag tctttttgtt tgtggttaat
     5761 actttctgga gcactaaccc gattttcttt ccatgaaatt caggtgcaac aaatttactg
     5821 gcggaaacca tatgctgcgt taattgtcca atcccggcac accccatcat ggcgtgaacg
     5881 acatttgcgc caaatttgtt ttccgttccg tcatttttct gaacacagac gctaagatat
     5941 tggattttac gtccgtcgtc agattcgcca gaaaactcaa taaatttggc tcctttttct
     6001 gattgcttta gttctgcttc agtaatggta atgatatgag caccagtttc gttaataaaa
     6061 ccaccttgcc ctgcggtcag tgctgcttct tcgttataag taaaaatcac gttgctcatg
     6121 cggcgttttc cttaatttga tgaacattat tgatgccgta gtaatcacaa acagtggcat
     6181 cgacgaaaga gagatcgtta tcaatctcat tggaatcaaa cattcccatt ggggatttaa
     6241 cagtgtctgc accgttgttt ttcgtggtga aaaagaactg gtcatcgcgg gtaagagtgc
     6301 gaagaactat agtaaacatg ccttcgacag tgattttctc gtccagcatt ttgccgatag
     6361 ttttcatttt cacgcgcccc ataggggttt cttcggtgtg cgcaagaaaa tagactctca
     6421 ggtcatcagg tgcatcctgt gcagccttaa tcacctccca tgcgtggcgg cctatctcag
     6481 taaatttatc aaacgatttt tcttctgagc gacgcataaa ctcattgctc atcacatact
     6541 ggaagtcatc aacaataacg attcttttcc cgtattcgtg agcacgctta attacggcaa
     6601 ctattacatc ccatttgtca gtggtaacta cggttccttt ttttgctctg gcatcccatg
     6661 ccagccagtc ttttgattta aatggtagcg gcttgcctat tggttttata agtattgctt
     6721 cctcgggatt gatatttctc atgctggttg attttccggt gccagactca ccgagtatta
     6781 atgtcgcagt tcccataatt tgcctcagaa tggtaattcg gatggggagg aaagaaactc
     6841 gcgctcattc atgcgctctc tttgtgcctg ccataaacaa agttgtttct ttgatttatc
     6901 tcccgcttta cgccagtaac gagcctcagc aatgtgatat tctcttttta atcgacttaa
     6961 ttctggagtt ttcgccagtt ctaccggaat cattttgacc tccattttct gtaggcttcg
     7021 atggcctcac gaaacatctt ttcatcgcca ataaatgtgg cgatagtgaa tttagtctgg
     7081 atagccataa gtgttttatc catttttggg aactcctggc tgattaagta cgtcgatgag
     7141 tcgtttccat ccgtcacgta atttacgggt gattcgttca agtaaagatt cggaagggca
     7201 gccagcaaca ggccaccctg caatggcata ttgcatggtg tgctccttat ttatacataa
     7261 cgaaaacgcc tcgagtgaag cgttattggt atgcggtaaa gccgcactca ggcggccttg
     7321 atagtcatat catctgaatc aaatattcct gatgtatcga tatcggtaat tcttattcct
     7381 tcgctaccat ccattggagg ccatccttcc tgaccatttc catcattcca gtcgaactca
     7441 cacacaacac catatgcatt taagtcgctt gaaattgcta caagcagagc atgttgcgcc
     7501 agcatgatta atacagcatt taatacagag ccgtgtttat tgagtcggta ttcagagtct
     7561 gaccagaaat tattaatctg gtgaagtttt tcctctgtca ttacgtcatg gtcgatttca
     7621 atttctattg atgctttcca gtcgtaatca atgatgtatt ttttgatgtt tgacatctat
     7681 tcatatcctc atagataaaa aatcgccctc acatcggagg gcaaagaaga tttccaataa
     7741 tcagaacaag tcggctcctg tttagttacg agcgacattg ctcacatagc agactcgtaa
     7801 atctgctata ggcgcttatt cgcatcgcat gacaacatca aatttttcga ggttactttg
     7861 tcgcaacaat ccttcttcga cgcggtcagc ttttctataa ttatcaaatt cgaaatgttt
     7921 aattacttct ttcgtttctc gctctataac ttcaacaatg tatttcttat tcatcattct
     7981 tccccaagag ctttgctaat tgctgactgt gctttcgata cttcatcagg ataatggtca
     8041 ttccagtttt gcttgtatgc cttgtttagc atcgctttca gggcatttaa aaggtcaggt
     8101 gctgccgcta ttagattggc atcttcaatg cattgaactt catcacagat tgcaatagac
     8161 gaacgccagc ctgtgccatt ttcaagagag tctgcctgga tgattttaat ctcatcgcca
     8221 tccatcatta tttcccactt accttcagta cctttaaatt ccatgttagc ctctgttgtt
     8281 tatgccaaaa ataaaggccg actattcgtc cttatctaca ttcctgaacc aaacgcagat
     8341 cggaccgtct tctgtatcgt gaatcgaacc gacaaaccat ccttctccat ctggcatgct
     8401 tggctcccat ccactgatgt tttgattccc atcttcaaaa tacgcgtcaa ttaccgtttg
     8461 attgttgtcg ttttccattt caacaattga tgattcaatg ccatgctgct tgcagaaaga
     8521 tctgaactca tcaactgaaa ttacctctct atccccaaac aggttggcgt attctgggtg
     8581 cgtccagtag ccatcctcgc ttcgctctac tactaatgct tccatatctc acctcaaata
     8641 agtggtttgc tgcctaattt cattttctgg cgaccaacac aagtcacacc catttcactg
     8701 cgtggctttc tgtaccatgt gcgcttattc tttcgctcaa tacgttgcag gttgctttca
     8761 atctgttcgt ggtattcagc cagcaccgta aggtctatcg gattcagtgc gctttctact
     8821 cgtgatttcg gtttgcgatt cagcgagaga atggggcggt taactggttt tgcgcttacc
     8881 ccaaccaaca ggggatttgc tgctttccat tgagcctgtt tctctgcgcg acgttcgcgg
     8941 cggcgtgttt gtgcatccat ctggattctc ctgtcagtta gctttggtga ttggatggcc
     9001 ggcgctgaac cccggcttac tggttagagc gcccgcacta ccagtgacgc tgtcttgagg
     9061 cgcagattgg ttactgcttg ccatgagcgc tgtttataca ttggtcgagc atcagcctgc
     9121 tcattcatcc aatcccaaag ccttctgctt tgaatgctgc ccttcttcag ggcttaattt
     9181 ttaagagcat caccttcatg tgggttagtg cgtcctgctg gtgtgtttag tatcaccgct
     9241 agtggtattt gtgtcaacac cgccagagat aatttatcac cgcagatggt tatctgtatg
     9301 ttttttatat agatttattt tttgcagggt tgtgtggctt gggaggtgat cgagagatca
     9361 gaattgcgtt gtttagtgag ttgcacctat taattttaaa ataaatacaa ttggttatgt
     9421 gtttttaggt ggggcgatcg tgaggcaaag aaaacccggc tcggtggccg ggttgatggg
     9481 attatcgttt atttctttga tgtttctttg cagactcagc ttgagtgttt ccatacctgc
     9541 gtttaccgct attgtttttt cccctaggct tgcctttttt tcgatcttcc caccagtcag
     9601 gtgccgggtc tggctcaaca tgtaaagcat cggcaagtcc tgatatcaag ctgtatgact
     9661 gaactccatc aatgtgctct gacagtgatg gggtaacatt ctgtcttagt ggatcaagaa
     9721 taaaatcaat acctttaatt ctggcgtgtt tggcggcagg cacaaagtct gagtccccag
     9781 caacaaggac aattacatca acaagttttt cataagctaa agtcgtgata tccatcccta
     9841 gcttgatatc aacttgtttt tgtttgatgt catagtaaaa atcatcattt gtcagttcat
     9901 cccatttttt cgttcctttc atcagagcat caagggagaa tgtagttagt tgccatcgct
     9961 tattatcaac aagattccct aatcttaagg ctgtttttcg agtttttctt aactcttcat
    10021 gcagctccgt tctcagaata tatgattttt cgagtttgaa attcttgcga ccaggcgtct
    10081 tattgccagg ctcaggaagt ggaaggcgtg tttgaatgtc gagtggtgga cagtcataga
    10141 agtaaattct ataaagctca agcggttccc tacgttcttg tgattgacgt tttccattaa
    10201 gatgggaaag aaccattgac catattactt tcattatgca ttgcgcagtc agttcatgct
    10261 cggcgaagtg tttacgatgc gtagcatgaa cacgctgcat gaaaaaaccc gcatcaatta
    10321 aaattgctgc tttcttcata agagtcccaa aaaaatagcc cagagccgtt atgcagatat
    10381 taacaattgt ctgcgaacgg ggctgggctt ggtttaatta atctatgcca agttgactgt
    10441 gccgtcaaca taaaacttac ttcctatcac ccaaacgtct cttcaggcca ctgactagcg
    10501 ataactttcc ccacaacgga acaactctca ttgcatggga tcattgggta ttgtgggttt
    10561 agtggctgta gaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca
    10621 ccaccaagtc tggctataca gaaatcgcct ggctcaacag cctgctcagg gtcaaccaga
    10681 attaacatcc cgtcaggaaa gctgggcttg gatcctgttg gtgcggtcat ggaattacct
    10741 tcaacctcaa gccagaatgc agagccactg gcttttttgg ttgtgcttac ccatttctcc
    10801 gcatcgcctt tggtaaaggt tctaagctca ggcgagaaca tcccggcctg aacatgagaa
    10861 aaaacagggt actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc
    10921 tcgtagattt ctctggcgat tgaagggcta aattcttcaa cgctaacgtt gagaattttt
    10981 gcaagcaatg cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg
    11041 cctgactgcc ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt
    11101 ttcttttttt cataaatagc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat
    11161 ggtttctttt ttgcgctcat acgttaaatc tatcaccgca agggataaat atctaacacc
    11221 gtgcgtgttg actattttac ctctggcggt gataatagtt gcatgtacta aggaggttgt
    11281 atggaacaac gcataaccct gaaagattat gcaatgcgct ttgggcaaac caagacagct
    11341 aaagatctcg gcgtatatca aagcgcgatc aacaaggcca ttcatgcagg ccgaaagatt
    11401 tttttaacta taaacgctga tggaagcgtt tatgcggaag aggtaaagcc cttcccgagt
    11461 aacaaaaaaa caacagcata aataaccccg ctcttacaca tcccagccct gaaaaagggc
    11521 atcaaattaa accacaccta tggtgtatgc atttatttgc atacattcaa tcaattgtta
    11581 tctaaggaaa tacttacata tggttcgtgc aaacaaacgc aacgaggctc taagaatcga
    11641 gagtgcgttg cttaacaaaa tcgcaatgct tggaactgag aagacagcgg aagctgtggg
    11701 agttgataag tcgcagatca gcaggtggaa gagggattgg attccaaagt tctcaatgct
    11761 gcttgctgtt cttgaatggg gtgtcgtcga cgacgacatg gctcgattgg cacgacaagt
    11821 tgcttcgatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
    11881 gatggaattc tgaggtcatt actggatcaa tccacaggag tcattatgac aaaacaactc
    11941 agtccttacc aggacaaaat tcacaaacac atactacgtg atcgcttcct gtccagcttc
    12001 aagcagcctg gtcgattccg ggctgagttg gaaaaagtga agctgatgca gaaggagaaa
    12061 ggtcatgagt aatcttgcaa ccgtaacaca tttaaggcct tcacaacggc ctgtggagcg
    12121 tcgtgtggca gaagttgaag atggttatac ccgtcttgca aatgccctgt atgaagagct
    12181 tatcggcgca gatttaacga aaaatcagag caaggttgcc cacgccatat gccgtaaaac
    12241 atacggctac ggtaaaaaga tggatcgcat ctctgatagt cagttagctc aaattaccag
    12301 gctgccaaga cagaaggtaa acaaggccaa gaatgagctt atcgcgatga aggttatcct
    12361 tcgcgaaggc cagcaaatcg gtcctaacaa gaacatcgag gaatggcaaa tcgaagggtg
    12421 tcactactct ggtgataatg tcactgcatt ggtgacaaaa agtgtcacca aaacggtgac
    12481 agcgctgtca ccaaaacagg gacacacaaa agaaactatt acaaaagaaa aaagaaataa
    12541 taaaaacact atgtccgaaa gtgttcggac ggagtgtgaa aaatcacctg accgtcacga
    12601 agaaaccgac aaggcattcg aggaaatatt ctggtgtgca ggcatgcgga aagccgggaa
    12661 gaaaaacgca gcttcggcat tcagaacaca gttcagggaa tggcgtaaaa ctaccagggg
    12721 tacggcaagc gagtttgcca cgatgctggc agaagacatc gcatgcagga atggtaagca
    12781 gttcggattc gacaggttgt taccatcgag ctacctgaac ggtcaacgct ggaacgacga
    12841 gaagccagaa actattcaac cacaatctaa accatcatcc gcaatcaccg tatcgaaaac
    12901 tggctacgtg tttttcgaca ggtgaaccat gaaatcaaaa atcaaatcgc tactggtcgc
    12961 tggttataac cacggctggt taagtatttc gtttgtcgat ttctggttta aaaatctcaa
    13021 tctgagggaa tcatgacgcc aagtgaactt agcgacctgc tttgggcgca ggttgacagg
    13081 gtggctccgc acctgttgcc aaacggcaag aaagaggggc atgagtgggt tgccggtaac
    13141 gtcaacggtg acaagggaaa cagccttaag gtcaacctta gcggcaagaa aaaatgggct
    13201 gatttcgctg agggagacgg cggtgacatg cttgatttgt ggatggcatg tcgtggaatt
    13261 aacctgcatc aggcgatgca ggaagcgaaa gcatttctcg gaatcaagga tgacgatcac
    13321 catttcgatg ccaaacgtga gaagaaattc tccagacctg atcgcaagaa gatcgcccgc
    13381 tacgttacca gaacagaatc ccatcttgag tacctgcaat cgcgtggcat atcgccagaa
    13441 atcgtaaagc gctacgaggt tgtcagcggc aaggtgtgga atggagagcg ggaactggat
    13501 gctctggtga ttccgtacaa acgcgatggt gagttgttgc aggtaaagcg aatcagcact
    13561 gagcgcccgg acgggaagaa agtcattatg gcagaaggtg attgcgaacc ttgtctgttc
    13621 ggatggcagg ctctggacgc tggcgtgagg gcggttgtac tttgcgaagg cgaaattgat
    13681 tgtatgagct atgcgcaata cggcatctcg gcgttatccg tgccgtttgg tggcgggaaa
    13741 ggcgctaagc aacagtggat tgagtttgag tatcacaacc tcgacaggtt tgaggaaata
    13801 ttcatctcga tggatgttga tgatgttggt cgtgaagccg caagggaaat cgcaagccga
    13861 ctcggtgaac atcgttgccg tcttgttact ctgccgtaca aagacatcaa cgaatgcctg
    13921 atgaacggtg ttaccgagga tgaaatctgg cagtacatcg gcacggcatc ctacttcgat
    13981 cctgaagaac tctacagtgc gcgagagttt taccaggaca ctatcaacgc tttctacggc
    14041 aagcagcagt atctgtttaa tccaccgtgg gaatctctgg cagataaatt ccagttccgt
    14101 gaggccgagt tgacgctggt caatggtgtg aacggtcacg gaaaaacgga ggttgtcggg
    14161 catatggcac ttgaggcaat gcgtcagggt gtgaagacgt gcatcgcgtc acttgagctg
    14221 aagcctggta ttctccttaa gcgacttacc cgtcaggcaa cgtgctgcaa gatgccgcca
    14281 gtgcttgaaa ttgactctgc atttaaattc tatgacgaaa gactttgggt gtttggcctg
    14341 acaggaacgg cgaaagccga caggctgatc gaaatattcg actacgctcg ccgacgatac
    14401 gggatccagt tattcatcat cgacagcctg atgaaatgtg gcataggcga cgatgactat
    14461 aacgggcaga aggcgtttgt tgactcgatt tgcgacttca aaaacaaaac aaactcccac
    14521 gtcattctcg ttactcactc gcgaaaagga gacagcgaag aaaaaccaac cgggaaaatg
    14581 gacgtaaaag gctctggagc gataacagac ctgacagaca accttttcat catctggcgt
    14641 aacaaggctc gcgagagagc gttacagaga gttcagagtg gtgaaaagat gtcagagaag
    14701 gacgaacagc tactggcgtc tcctgcatct gttttgatgc ttgaaaaaca acgtaacggc
    14761 gaaggttggg aaggtggtgt cccgttgttc cttgacgagc aatcgcacca gttcctgcaa
    14821 cttgaatcag gatcgccata tagctacatc gccaatatgc cgaaatcgga atatgacgag
    14881 gcgtggcgac aggaaaacgt gacggagtat taaatgacca tctacatcac tgagcttgta
    14941 acaggcctgc tggtaatcgc aggccttttt atttggggga gagggaagtc atgaaaaaac
    15001 taacctttga aattcgatct ccagcacatc agcaaaacgc tattcacgca gtacagcaaa
    15061 tccttccaga cccaaccaaa ccaatcgtag taaccattca ggaacgcaac cgcagcttag
    15121 accagaatcg gaagctttgg gcttgccttg gtgacgtttc gcgtcaggtt gaatggcatg
    15181 gtcgctggct ggatgcagaa agctggaagt gtgtgtttac cgcagcatta aagcagcagg
    15241 acgttgttcc taaccttgcc gggaatggct ttgtggtaat aggccagtca accagcagga
    15301 tgcgtgtaag cgaatttgcg gagctattag agcttataca ggcattcggt acagagcgtg
    15361 gcgttaagtg gtcagacgaa gcgagactgg ctctggagtg gaaagcgaga tggggagatc
    15421 gggctgcatg actatcaaat caaatacgcc agcacacgac aaggactgct ggcaaacgcc
    15481 gctctggctt tttgatgcac tggatattga gtttggattc tggctggatt cggcagcgag
    15541 cgacaaaaat gctctgtgtg ctcactggct aactgaggcc gacgacgcgc tcaattctga
    15601 gtgggtaagc cacggtgcaa tctggaataa cccaccgtac agcaatatca ggccgtgggt
    15661 ggaaaaagcc gctgagcagt gcatacaaca gcgacagacg gtagtgatgc ttgtgccaga
    15721 ggatatgtca gtcggatggt tcagcaaggc tctggagagt gtcgacgaag ttcgcattat
    15781 cactgatgga cggattaatt ttatcgaacc atcgacgggg ctggagaaga agggaaacag
    15841 caaaggttcc atgctgctga tttggcgacc gttcatcagt cctcgacgga tgtttactac
    15901 cgtatccaaa gcggcattga tggcgatcgg gcagggcgtc aggagggctg catgagacga
    15961 cagcggcgaa gtttcaccga catcatctgc gaaaactgca aataccttcc aacgaaacgc
    16021 tccagaaata aacgcaagcc aatcccaaaa gaatctgacg taaaaacctt caactacacg
    16081 gctcacctgt gggatatccg gtggctaaga catcgtgcga ggaaatgaca atggattatt
    16141 cacagttaag tgattttgaa attaacgtgg cggtattcga agccattcat aacggatcac
    16201 cggattacaa agaaggtgag aatggcgcga tggtgtttat ctcatttgag ggagacattg
    16261 taaacggaga cgcagttgaa gtagaggttg agcgcggatc ctttaaccca tgcgcaaacc
    16321 cagcagacgc atggccgatt atcactgaaa acaacatcag cataatttta gacaatccct
    16381 caatgccgtg cgctacagac aacgcaaggg acttgtttga tgatgccgga ccgaatgttg
    16441 gtgtcgcata tgacaatcca ctccgtgccg ccatgattgt ctttctcatg attcaggacg
    16501 ccaataatgc ttagcccatc ccaatccctt caataccaga aagaaagcgt cgagcgggca
    16561 ttaacgtgcg ctaactgcgg tcagaagctg catgtgctgg aagttcacgt gtgtgagcgt
    16621 tgtatatatg aatgcttaaa tatggtggaa cataatgaga aatataagca acatagacga
    16681 attaagaaat aaattatcat atgatgatgt tttaggtgta cttagatgga aagtgtctcc
    16741 ttctaataat gttaaggttg gcagtgttgc tggttacatt aggagtgatg gttataggat
    16801 gttaactatc gatggtgtaa ttacttatgc tcaccacgtt atatggatga taaataatgg
    16861 tgagattcca ttagggtata aaattgatca tataaatgga gtaaggtcag ataataggct
    16921 atctaatctt cgattggtta cacaccaaca aaatgcacag aaccaaaaga gaaaaataac
    16981 caattcgtca ggagtgactg gagtgtattt caataaagag agatgcaagt ggattgcgaa
    17041 tatatgcgta gatgggcgca ccaaatatct tggaatattt gctagcatca ttgacgctat
    17101 tgcagaaagg aagagggcag aaaaagaact gggttttcat gaaaaccatg gcagaccata
    17161 actgcgcaga actgatgagc gatccgaata gctcgatgca cgaggaagaa gacgatggct
    17221 aaaccagcgc gaagacgatg taaaaacgat gaatgtcggg aatggtttca ccctgcattc
    17281 gccaatcagt ggtggtgctc tccagagtgt ggaaccaaga tagcactcga acgacgaagt
    17341 aaagaacgcg aaaaagcgga aaaagcagca gagaagaaac gacgacgaga ggagcagaaa
    17401 cagaaagata aacttaagat tcgaaaactc gccttaaagc cccgcagtta ctggattaaa
    17461 caagcccaac aagccgtaaa cgccttcatc agagaaagag accgcgactt accatgtatc
    17521 tcgtgcggaa cgctcacgtc tgctcagtgg gatgccggcc attaccggac aactgctgcg
    17581 gcacctcaac tccgatttga tgagcgcaac atccataagc aatgcgtcgt atgcaatcag
    17641 cacaagagtg ggaatctggt tccgtaccgg gctgagttaa tccggcgcat aggcatcgag
    17701 caagtcgagg caatcgaatc tgaccataag cgccatcgct ggaccaccga agagtgcaaa
    17761 gcgattaagg cggagtatca acagaagctt aaagacctgc gtgacagcag aagtgaggca
    17821 gcatgacaaa cgtagcgtgc ctgacaatct acctcgttgc tgtgttctat ttcctcatca
    17881 gggaggtggc aaggcataag agactaaaaa tggcgtatgg cataaagatt cgggctattc
    17941 gatatgccga aaagtaccga atgcttaagc gattaatgag aggctaggcc atgacagaca
    18001 taagaagaga ggtctgtgaa gagtatctgg atgctctgat aacagtggaa ttatccgtgc
    18061 gattcgctca tctcgaagac aggaaagtaa acgcaaccat ccgcgcaaca gtaactgagt
    18121 tactcaaacg tatccgcgac aagaaaatcc gcgccatctt tgcaggctta tcccatcagc
    18181 cattccctga tggagccttg aagatgatgc gtcgccagtt agacagctta gtaggagaac
    18241 ccgtatgagc actgttacta acatccagca agtcaaatgg cagcgtcatg cagatgagca
    18301 atcacttaaa tcgctcgatg caaagattaa agatgctgaa aacgctttgg tagttctgct
    18361 tcatcatcct cgggagttag ttaatcgcct ggatttgaat aagcttgatg gtccggaggt
    18421 tgcctaatgg gtgtgcgtga acttaacctg accaaagacc agcatgactg gcttaatgga
    18481 tggcttgaat tgtggggcgc gtgggtttac tcggggcgac tggaaaaacg catgagcagc
    18541 gttatagcgc agtttatgga gagagtcgaa ccatcacgag taatgacaag gccaatgtgc
    18601 aatgacgatg acggaatgtt gatttctcag gtcgtagatt ccgttatgcg catcgacaca
    18661 aaggcctttg gtattcttct cagctactac tctcacggtt cttccaagag agcaattgca
    18721 tcgtactatc acgccaccgc aaagccacgc aaaatggccg gaagaagcgg agagggttgg
    18781 cgacgaccat cacttgcaac atgcaggaat gaaatcgacg atatcctcaa agcttcactc
    18841 tttgttttgt accaacctat gcaagatgct tttaaaagtc gcaaacgtgt tgagaagatt
    18901 aaacatgttg cacctaaaat gcttgacaac cttttagcca tttagccata attagagggt
    18961 aagctgccgt tagtgactct taagttgcaa cggtggcttt ttttatttgc acaacaggta
    19021 agagcattaa agaactggca aagagcttaa cggtctgcga aagcatttct tagtggcaca
    19081 actggccggt acaactgagt gctctttccg gtgtggtgaa tgcgcaggct gatgcgcgca
    19141 ggagagcttc ggaagaacaa ggtgcctgta tacaagccgg agatcagcgc cggccaccac
    19201 agtcaaatcc acccagagca aaacccacca agaaccagac ctaaccaact cattgctgac
    19261 actctgtggc tacggtggtc tagtgcgctt taaaaaagaa aaccctgcat caatggctgg
    19321 gcttcgtgat atgagcggca tgtattgttg gcgcaatcca cgcctgattt gctcatgaat
    19381 gcggtcacga acaaacccgt tacaaatcaa ccgtaacccg gatttgttca agcgaccata
    19441 tccataattc ctaatttgaa cagatcccct tattgggggt aagacatgaa gatgccagaa
    19501 aaacatgacc tgttagccgc cattctcgcg gcaaaggaac aaggcatcgg ggcaatcctt
    19561 gcgtttgcaa tggcgtacct tcgcggcaga tataatggcg gtgcgtttac aaaaacagta
    19621 atcgacgcaa cgatgtgcgc cattatcgcc tggttcattc gtgaccttct cgacttcgcc
    19681 ggactaagta gcaatctcgc ttatataacg agcgtgttca tcggctacat cggtactgac
    19741 tcgattggtt cgcttatcaa acgcttcgct gctaaaaaag ccggagtaga agatggtgga
    19801 aatcaataat caacgtaagg cgttcctcga tatgctggcg tggtcagagg gaactgataa
    19861 cggacgtcag aaaaccagaa atcatggtta tgacgtcatt gttggcggag agctattcac
    19921 tgattactcc gatcaccctc gcaaacttgt cacgctaaac ccaaaactca aatcaacagc
    19981 cgccggacgt taccagcttc tttcccgttg gtgggatgcc tatcgtaagc agcttggcct
    20041 gaaagacttc tctccgaaaa gccaggacgc tgtggcattg cagcagatta aggagcgtgg
    20101 cgctttgccg atgattgatc gtggtgatat tcgtcaggca attgaccgtt gcagcaatat
    20161 ctgggcttca ctgccgggcg ctggttatgg ccagttcgag cataaggctg acagcctgat
    20221 tgcaaaattc aaagaggcgg gtggaacggt cagagagatt gaggtatgag cagagtaacc
    20281 gcgattatct ccgctctggt tatctgtatc atcgtctgcc tgtcatgggc tgttaatcat
    20341 taccgtgata acgccatcgc ctacaaagac cagcgcgata aagccacatc catcatcgct
    20401 gatatgcaga agcgtcaacg tgatgtagca gaactcgatg ccagatatac aaaggagctt
    20461 gctgatgcta acgcgactat cgaaagtctc cgtgatgatg tttctgctgg gcgtaagcgg
    20521 ctgcaagtcg ccgcctcctg tgcaaagtca aagaccggag ccagcggcat gggcgatgga
    20581 gaaagcccaa gacttacagc agatgctgaa ctcaattatt accgtctccg aagtggaatc
    20641 gacaagataa ccgcgcaggt taactacctg caggaataca tcaggacgca atgccttcga
    20701 tgatagcgat aattttactc atcatccttc acatctggct ctgtagacag ggtggtgatc
    20761 acttctggag tgaatccaga ttaaacatct cattgctgat gcttgatatt gagcatcttg
    20821 cgcgcggtaa ggggctgcgt tgagataaga gccagtcatt acaaatacca ggatttagcc
    20881 tcgcattcgc ggggtttttt attcccaact ccataggtga ttttatgacc cagcatattg
    20941 gcgtaaaact gattaacgcc tttccgatga cgagacaggc atataacgat tttcgtggct
    21001 ggcagcttcc tgccggagaa aacggcgagg acgaaggcta tctggttgaa tatctggatg
    21061 gcggaaaacc taacaccgat cgctttgatg gctacgttag ctggagtcca aaagaagtat
    21121 tcgaaagggc ttatcgtccg gtatcaggac taagtttcgg ccttgccatg gaagcgttaa
    21181 aacagggcaa aagtttgcag cgggcaggat ggaatgggaa agaccagttt gtttatctca
    21241 tgaaagggga aaaattagcg tctgcgttgg gttatggctt tggcgaatat gttggcgagc
    21301 caactttcaa tgacacgctt gtattgaaaa actcacagaa ccgccttgct acatgggttc
    21361 catccattgg cgacctgatg gctgaagact ggcaaatcat ttaaccatgt agtcattaca
    21421 aagctcatct acgggtgggc ttgataatga aaccggagtt aatttctggt cactaattaa
    21481 cggcagtaca gcgaaacaac caaagccagt aagtggggaa ataacactgg cagccactga
    21541 aagatgaacc tcctgcctta tggcaaaaaa gattctttgt ggtggcggac tgatggaaag
    21601 acatcggtta ttgcagaggc cattcaatga gtggtctcga caatggctta taccctacac
    21661 aggataactt aactgatatc ccttttaacg gataaacgga gccaacaatg gcagagatta
    21721 ctcccatgac tgaagaacag aaattccagt tagagattta caaactggtc atgaaccaga
    21781 acgcagccgc agaagaagca tttcagttca ttggcactga cgagctgaag cttgagctat
    21841 tcaaaattca cttccagtca ggcggcgcta attcagatat cacgatccgc acattcgaag
    21901 cggtgcgtaa atcgaaggaa gcgttagacc tgttcactac cggagcataa acatggcgcg
    21961 cccaacaaag tatcaagagg cgtatgccga acaggcacgc aaactgtgct tgctgggcta
    22021 caccgatgca gaacttgctg atttctttga agtcagtgag tcaactatta acaagtggaa
    22081 gcttgattat cctaagtttt cggagtccat aaaaaagggt aaggccgtcg ctgatgcaga
    22141 agttagtgat cgtctttatc aacgcgctat gggcttcgtg gctccagata tcgatattcg
    22201 tgttattgaa aacagaattg tcgaaactcc gcttgagaag tattacccgc ctgatacaac
    22261 cgctgccatt ttctggctta agaaccgaca gaaggataaa tggcgcgaca aggttgatca
    22321 cgagctaaca ggcaaagacg gcggcgcaat ccagattgaa acatcaccga tgagcactct
    22381 attcggaaaa tgacctcgat taatcctatc tttgaaccgt tcattgaggc gcatcgctac
    22441 aaagtcgcca aaggcggtcg aggtagcggc aaatcatggg caattgcgag gctgcttgtt
    22501 gaagcggcgc gtcggcagcc tgtgcgtatt ctctgcgctc gtgaactgca aaacagtatc
    22561 agcgattcgg taatccggtt gcttgaagat accatcgagc gtgaagggta tacggctgag
    22621 tttgaaattc agcgttccat gattcgtcat ctcggaacga atgctgaatt catgttctac
    22681 ggcatcaaaa acaacccgac gaagattaaa tcgctcgaag gtattgatat ctgctgggtg
    22741 gaagaagcgg aggcggtaac gaaggaatca tgggatatcc tgataccaac catccgtaag
    22801 ccgttctctg aaatatgggt gagctttaac ccgaagaaca tcctcgacga tacctatcag
    22861 cgattcgtcg taaatcctcc tgatgatatt tgtctgctga cggtgaacta caccgacaac
    22921 ccgcactttc ctgaagttct ccgtctggag atggaagagt gtaaacgcag aaatccgaca
    22981 ctgtatcgtc acatctggct tggtgagccg gtaagcgcaa gtgatatggc aatcatcaaa
    23041 cgtgaatggc ttgaagccgc aaccgatgcg cacaagaaac tcggatggaa agcgaaaggc
    23101 gcggttgttt ctgctcatga cccatcagat acagggccag atgctaaagg ttacgcatcg
    23161 cgtcacggtt cggtagttaa gcgcattgcc gaaggcctgc tgatggacat caatgaaggt
    23221 gctgactggg caacttcgct ggctattgaa gacggcgctg accactattt gtgggatggt
    23281 gatggtgttg gtgcggggct acgcagacag acaacggaag cgttctccgg taagaaaatc
    23341 accgccacga tgttcaaggg cagcgaatcg ccatttgatg aagatgcacc atatcaggct
    23401 ggagcatggg ctgatgaagt cgtgcagggc gacaacgttc gcactattgg cgatgtgttc
    23461 cgcaataagc gagcgcaatt ctattacgcg ctggctgaca ggttgtatct gacatatcgg
    23521 gcggttgtcc acggtgagta tgcagacccc gacgacatgc tgagcttcga caaagaagcg
    23581 ataggcgaga agatgctgga gaagctgttt gcagaactga cgcagattca gcgcaaattc
    23641 aataacaacg ggaagctgga gcttatgact aaggtcgaaa tgaagcagaa gctcggtatt
    23701 ccatctccta acctggctga tgcgctgatg atgtgtatgc attgcccaga gtcggctgcg
    23761 caacccgact attccagtta ctcaattcct tgtggtgtag gttgatatgg cagaaaaaaa
    23821 gatgactgac tggcatcgca aggtgctgtg caactttgat aatgcctggt cagcaacgca
    23881 ggatatgcgt gagcagatta ttgaggctca acgtttcgtc cgggtgtccg gcgcacagtg
    23941 ggaaggcagc acaaacgctg gttactcatt tgatgaaggc aggttcgagc attatccgcg
    24001 ttttgaattg aataagattg cccgtgaatg tgatcgcatc attggtgagt atcgacagaa
    24061 tcgcatcagc attaaattca ggccgaagga cgacaaggca tcggaagcgt tagccgaaaa
    24121 gatgaacggc aaattccgcg ctgactatca ggaaacatcc ggtggcgaag cgtgtgataa
    24181 cgcatttgat gatgccgtaa ctggcggatt cggttgtttc cgcatgtgtg ccgattacga
    24241 agatgaaatg gatccgagta acgagcagcg acgcatcagc cttcttcctg tttacgaccc
    24301 agcgacatgc gtcttctttg atcaggacag caagcaatat gaccgatctg atgctatgtg
    24361 ggctatggaa atgttctcca tgacgcctaa agcgttcgag gctgaatacc ctgattccat
    24421 tgcggcaagc ctttctcgtg atgacactgg tactcagtat gactggtcaa cgcccgatgc
    24481 catctatgtt ggacgctact acgaagttcg catagagaag gtgaagctca cggcgtggcg
    24541 caaccctgtt agcggagaaa cggcaatcta tgatgaggag caaatcaaag atattgtcga
    24601 cgagctgacc gatggtgcat tcgaactgat tggcgagcga acggtgaaga agcgccgcgt
    24661 ttattgcggc cttctgtctg gcgctgaatg gctggaagaa ccgaagcgta ttccgggcga
    24721 acatattcct ctcatcccgg tatatgggcg tcgctcattt gttgataatc aggagcgaat
    24781 cgaaggccac gcagcaaaag cgatggatgc acagcgtctt gagaacctga tggtttccat
    24841 gattgcagat aacgctactc aggctggcgg tgatggcatt cctgtagttg atgttgacat
    24901 gattcctggt cctctcgcca ctcattgggc ggagcgcaac aaaaagcgcc cggcgtttct
    24961 gccgatggtc agtctgaaaa acaaaaacgg agatattact gcgcaggctc aggtcagcag
    25021 ttatacgcct ccgacacaaa tgccccccgc acttgccggg ctgttgcagt acaccggaac
    25081 ggctattcag caaattacag gcgcgtcgca gcttgagaac atgccgagca acgtcgccac
    25141 cgataccgtt gatagcatct ttaaccggat ggacacgcag tcctatatct acatggacaa
    25201 catggctaaa tccatgcgcc gcgctggcgt cgtgtggctt tctatggctc gtgaagtcta
    25261 tggcagcgat acgccaatgc gcattgttaa tgaggacggc agcgatgacg tggcgttgat
    25321 gactggtgaa gtggttgacc gtcagacagg gcaggttatc gcgcttaacg acctttcgca
    25381 gggtaactat gaagtgactg tcgatgttgg tcagtcgttc gctactcgcc gtgatgcaac
    25441 ggttaagtcg ttactttcca tgctggcact tatcccacca ggaacgccga agcacgacct
    25501 tgtatcgtcg atgattctcg acaatatgga cggcgaaggg atggacgacc ttaaagaata
    25561 caaccgcaat cagttgcttc tgtctggagt tatcaagccg agaacaccag aagaacagca
    25621 gatggttgag caggcgaaac aacaacaggc cagtcagcca gatccggcta tggtttcagc
    25681 gcaaggtcag cttcttgcag gtcaggctga attgcagaaa gcgcagaacg aacaggcagc
    25741 cattcaggtt aaagcattcc aggcacagac tgatgctcag gttgcagcgg caaacgttgt
    25801 gaaaatcctc gcatctgccg atagccagca gaaatctgat atccgcgagg ctctgaaact
    25861 gctcggacag ttccagcaac agcaaggaga caatgcccgt gctgatgcag agcttgtcct
    25921 gaaaagtcag gcacagggtc atgcgcagcg catggacatc agcagcatcc tgcaaaaatc
    25981 aactcagcaa caaccacagc agtaattaac ccataacgtg caatggctgt ctttatgagg
    26041 cctggcaccc tattgccttc cgatgggctg aacatcgagt aaacaggggt aacaaatgga
    26101 ccagatggca gaaaacacac cagaagttga aatcgaaacc gacgcgtcag agcagattcc
    26161 tgatgatgtc gaactggctg aagaagtcga aacagaagat ggtagtgagt cctcaggcaa
    26221 tgatgcagag gaggctactg aaactgatga cgacgaatca gaacaggaat tctactttgg
    26281 tgacgaaaag ctggattcgc caaccagcga agatggcgct gagcatggac tggtaaaaca
    26341 cctgcgcaag acgattaaag agaaagaccg cgagctgaaa gagctgatgc gtcagtctca
    26401 gaaacccgtc gagcagcagc cggcaatcac tcaaccaccg cgaatgccaa aactggatga
    26461 tgaggatatc ggtttcgatg aagaaatcta tcagcaacgc atggctaagt gggcagagga
    26521 taacggaaag taccagcaac aggagatggc tcgcaagcag aaggagcagg agcttcaggc
    26581 tgcttatcaa gagcgattat ccaaatatca gcaacgtgtt aaggctctca aggttcctgg
    26641 ctatcaggaa gcagaacagg ccgtactcga ggaaatcccc atcgagacac aaaacgcgat
    26701 cctgtttgag tcagagaagc cggaaatcgt tgttctggca ctcggtcgca acgctgaact
    26761 gcgcaagcaa ctggcagaag ctaccaaccc cgtagcaatt ggtcgtctgc tggaacgtat
    26821 cgaatcgaag gccagaatca tgccaaaagc aaaaaccacg gcagccacaa ccccgacagt
    26881 taaggggagc aacggcgcag taatcaataa cctcgacaaa ctgaaagcca aggcgctgga
    26941 aactggtgac tggacgccgt atttcgccgc taaaaaggca aaaaaataac ctatcggagc
    27001 attaagcatg gctaaccaat tagcaaaaga ccttgaaatc atgttcgaaa actacgttga
    27061 aggctttgag gccgcctgcg tagtttcccg taacgctaaa aaattccgtc ccggtgatac
    27121 agcaatgcag cgagcaggtg atgttctgta tcgtccgcag cattaccaca tgaacattga
    27181 ggaaggccta gacctcagcg gcaaaacgcc aacagcactg gttcagcgcc ttgttccttc
    27241 tgtgttcaag gagccgaaaa acattctgta cactctggat gcgcgtgaaa tgcgtgaccc
    27301 ggaacataaa actgaagctg gtcgcgctgc aggtatgcgc cttgctgcac agattgactc
    27361 tgacctgatt tccatggtta cgcagcgtgc tactaacgtg atcacgatgg ctgactcaac
    27421 cacaggttca cagggccgtg atttgtggaa ctgtgcggca ggtattgatg ccaccatgac
    27481 agcgattggt gtacctcagg gtatcaaccg tcgctctttc tggaacccct tcaactacaa
    27541 agaccttgct ggcgagcttg gtcaccgtgc ctatgctcag ggcgcaaccc tgacagcata
    27601 cgaaaaagcg cagatccctc cggttgcgtc cttcgatagc tacaagaccg atatttctgg
    27661 tcgtgttccg aagggtgcag caacttccat tacgctggca gcagcacctg cgcacaaggt
    27721 tgaagcgaaa gatgctaacg atatgccagt ggataaccga caggggacca ttacggtatc
    27781 tgctgaaggt ttgcaggttg gcgatgcgtt taccatcgca ggggtgaatt ccgtacacca
    27841 gatcaccaaa gataccaccg ggcagccgca ggtattccgc gttctggcag ttagcggaac
    27901 gacagtaact atatccccga aaattctgcc gcctgacaac gcggatgtcg ccagccgtcc
    27961 atatgcaaac gttgatgcta atgcggcaag tagcgcagca atcaccattc tcaacaaaaa
    28021 tgccgcaccg gctaacctgt tctgggctga tggttctgtt gaactgatgt acggcaaact
    28081 ggcgttcccg actggtcagg gtccacaggt aatgacagca accaccgagc agggcgctac
    28141 gctgatcatg tcttacgcct tcgaccacat caaaggtgta accactgcgc gtttcaccac
    28201 cctgtacggt tgctctgtac ttgttcctga atatacgggc atcgttattg ccgggcagta
    28261 attttggtgg ggcttcggcc ccatttttat tgggagaaga caatggcacg aacaatgctc
    28321 tataagcctg gcaacatgat cacctgtggt cagtttgctg tcgattacat cattgttgat
    28381 gacgaagaag ttaaatctca cctgaaaaaa ggctgggtaa aaactcctga agaaaccgca
    28441 acgaagcata aagtggctaa ggcggaagaa gatggcgaaa acgaagggtg atctcgttct
    28501 taaggcttta cgaaaagccg ggctgtattc caatgccacg ttgacagatg ccgaccctca
    28561 ggcaattgaa gatgccatta atgacctcga agacatgatg tcagcatggc aggcgaaagg
    28621 tatcgagctt ggatatcagt ttgctgatac agaaaacggc atcatgccgt tacctgacga
    28681 tgattcaggt atccctgcat gggcaaatga tggcgtcgct ttgaagctcg ctgtgcaggt
    28741 gtgcatggat aacgtcattc agccgtcgga tgctctcctg accgctgctg acagtgcata
    28801 tcagacaatc tgtatcgctt taaccaaaat accaccactt gagcggcgaa atgacatgcc
    28861 tcgcggtagt gggaacaaaa gcgcgtttac gtggaatcgg ttttacatcg agaaagatga
    28921 tccgagtacg tgaggtgaat aaatgccgat tcagcaactt ccgcttatga aaggtgtcgg
    28981 caaagacttc cgaaacgccg actatatcga ctatctgcca gtgaatatgc tggcaacccc
    29041 caaagaaatc cttaacatca gcggatatct tcgctcattc ccgggcattg ccaaacgctc
    29101 tgatgtaaac ggtgtatcgc gaggagtcga gtacaacatg gcgcagaatg ctgtttatcg
    29161 cgtgtgtggt ggcaagctct acaaaggaga aagtgaagtc ggtgatgttt ccggaagtgg
    29221 tcgtgtatca atggcgcatg gtcggacatc acaggcggta ggcgttaatg gtcaactggt
    29281 cgagtatcgc tatgatggca cggttaaaac cgtatcaaac tggcctgcag acagcggatt
    29341 cacgcagtat gagttaggtt cggtccgtga cattacgcgt ttacgtgggc gttatgcgtg
    29401 gtcaaaagac ggtactgatt catggtttat cactgacctt gaagacgaat cgcatcctga
    29461 ccgttacagc gcacaatatc gcgcagaatc gcagccggac ggcatcatcg gcataggtac
    29521 atggcgagac ttcatcgtct gctttggttc atcgacgatt gaatatttct ccctgactgg
    29581 tgcaaccacc gttggtgccg ctttgtatgt cgcacagcca tcactgatgg tgcagaaagg
    29641 gattgccgga acctactgca aaacgccgtt tgctgattcg tatgcgttca tcagcaatcc
    29701 ggcaacaggt gcgccgtctg tatacatcat cggctccggt caggtgtcac caatcgccag
    29761 cgcgagcatt gagaaaatcc tccgctccta cactgctgat gaactggctg atggcgtgat
    29821 ggaatcgttg cggtttgatg ctcatgagtt gctgattatc catcttccgc gccacgtcct
    29881 cgtatacgac gcatcttcaa gcgccaatgg tccgcaatgg tgtgtgttga aaacaggcct
    29941 gtatgacgat gtgtaccgcg ctatcgactt catttacgaa ggcaatcaga taacgtgcgg
    30001 cgataagctg gaatcggtta tcggcaaatt gcagttcgat atcagcagcc agtacgacaa
    30061 gcaacaggaa cacctgttgt ttactccgtt gttcaaagcg gataacgcaa gagtgttcga
    30121 ccttgaggtt gaatcttcaa ctggcgttgc gcagtacgct gaccgcctgt tcctctctgc
    30181 aaccactgac ggaatcaatt acggacgtga gcagatgatt gaacagaatg aaccgttcgt
    30241 ttacgacaaa cgtgttttgt ggaagcgagt cgggcgcatc aggaaaaatg tcggcttcaa
    30301 attgcgcgtt atcacgaagt cacctgtcac tctgtctggc tgccagataa ggattgagta
    30361 atggttgatt catcactgaa tgatcctgtt gtggttcagg ctacgcgcct tgatgcttca
    30421 attttgccac gcaatatatt cagccagtct tacctactgt atgtcataaa tcagggagct
    30481 gatgtcggtg caattgctgg gaaggcaaat caggctggtc agggcgctta cgatgcccag
    30541 gtaaaaaacg atgaacagga cgtcgaactg gcagatcacg atgcaagaat caccgcaaac
    30601 acaaaagcga taaatctact tgaggtcagg ttaacaactg ccgaagggaa gatagtcgta
    30661 ctgcgtagcg atgttgatta cttgctggat gaggttatcg atattcaggc gcatctggtc
    30721 actgttgacc aaagactgga taacgtagaa aacgatgtct ctggcattaa gagtgattac
    30781 gtatcgaaaa ccgtaaccga atcgcagtct cttgcgtcac cgctggatgt aaaaacatca
    30841 tattcagttg atggaattca ggtcgttgga gcaaggcaga ccggatggac tgcagccaca
    30901 ggtacacctc ttcttggctc attcaacgct aaccagtcat acacggtcgg cactacgtac
    30961 acacaatccg aagtcgcggc tctcgctaca ggtttgcagc aggcgcggca gcgtattctg
    31021 gcgcttgaaa cggcacttag attacatggg ctgattgact gatgattaca ttcaaaccaa
    31081 cgcgaaacat cgacctgatc gaagctgtcg gaaatcaccc tgacattatt gccgggagca
    31141 acaacggtga tggatacgac tacaagcctg aatgccgtta ctttgaggtg aacgtgcacg
    31201 ggcagttcgg cggcattgtt tactatcagg agattcagcc gctgacattc gattgccacg
    31261 ccatgtacct gccagaggtt cgtggcttca gcaaggaaat cgggctggcg ttctggcgat
    31321 acattctgac taacaccacc gttcagtgcg tcacatcgtt cgccgcacgc aaattccgcc
    31381 acggtcagat gtactgcgca atgattggcc ttaagcgtgt aggaaccatc aagaaatact
    31441 tcaaaggcgt ggatgacgtg acgttttaca gcgccacacg cgaagaacta atcgacttcc
    31501 tgaatcacgg gagatagcca tgttatatgc atttaagctg ggcagaaaac tgcgcggcga
    31561 ggaaccttat tgccctgaaa aaggcgggaa aggtggcagt tctgataaaa gcgcaaagta
    31621 tgccgcagaa gctcagaagt atgcagcaga cctgcaaaat cagcagttca acaccatcat
    31681 gaacaacctg aagccgttta ctcctctggc tgataagtat gtcggcagcc tcgagaactt
    31741 atcgtctctg gaagggcaag gtcaggcact taaccagtat tacaactctc agcagtacaa
    31801 agatcttgct ggtcaggctc gttatcagag tctggcggca gcggaagcaa caggtggatt
    31861 aggttccacc gcaaccagta atcagttagc aacaatcgca ccaacgctag gtcagcaatg
    31921 gctatctgga caaatgaaca attacaacaa cctggcaaat atcggtcttg gcgctcttca
    31981 ggggcaggca aacgccgggc aaacatatgc caacaacatg agtcagattt cacagcaaag
    32041 cgcggcgctg gcggcggcaa acgccaaccg accgtcagca ttgcagcagg gtgttagtgg
    32101 tgctgcatcc ggtgcgcttt tgggtggtgg catagccagt gctctcgagc tatcaactcc
    32161 gtggggtgct ggtatcggtg ctggtcttgg tctgcttggc tcgttgtttt aaggggtaat
    32221 caatggctac gtggcaacag ggtattaatt ctggtggttt tctggctggc attggtgcgc
    32281 aaaacgagaa tgcgccaaag gcaagcgaca ttaacgcaac gcttggtctg atccgcgaaa
    32341 acaatgaact ggctcgctca ggtgcaaata acgttggtct gaccgcgtta cgtggtctgg
    32401 ctggagttgc tgatatttat aagcaggaac agcaacagaa agcgattagt gcgttcaata
    32461 aggttcacgc tgatgcatgg gcttctggtg atccatcggg actatttaag tttgcccagg
    32521 aaaatccagc gtttgttgcg caggcacaac aggcgttttc cggtcttaat gagcagcaac
    32581 gcaacgatat gggcgattta gccatgaggg ctaacgtcgc tctttctcag ggacctgaag
    32641 cctacagtaa attcattact gacaacaagg acaggttaaa tcgcgttggt gctaatgctg
    32701 actggatgat tcagacaggt atccagaatc cagagcagct atcacacatg ctgactacta
    32761 tgacgctagg ggctgttggt ccggataaaa tgctggatta tcaggataag atggttggtc
    32821 gccagcagga gcagcaaaga attaacgaaa caatccgcaa taatgacatg acaaatgcca
    32881 gagggtgggc aagcaacaat attgcgcaac aaaatgtcaa tcttcgtcga atggaattag
    32941 aggacaagaa atacgacaga ctcatcgcaa atgaaactaa tgccttaaaa cttgctgaat
    33001 tgcatgacaa gagattgcag aatcagcaag ctatggagca ggcaaagcga gataaggctg
    33061 atgcgtacaa ctctggaatg gataatcttt ccagaacgat agagacggct acaaaagttc
    33121 ttaatagccc aggattcact ggatatttcg gaacaaacct aaacccacta tcgagtagat
    33181 tcattccagg aacagaggct gctgatacag aaactctggt tgacacactg aaatctcagg
    33241 gattcttatc tggcattcag cagatgaaag ggatgggggc tttaagtaat gccgaggggc
    33301 aaaaggtaat ggatgctatt ggtagtttgt ccccaaatca gtctgaaaaa tcagccagag
    33361 cagctatcaa aacaatcata aaaaccactg agatggctca gaaacgtatg caacagaaat
    33421 acgggaagga catacaaccg tctcaacagc agctttctga tgatgacctg attaataaat
    33481 atctcggagg gcagtaatgg cctatagtcg tgaacaattg atgacggcgt taaggaatgc
    33541 tgatgctgct ggtgatactg agggagcacg tcgcatcgct cagattctgt cctccagtaa
    33601 tcaaccaact caaaaccaaa agcagcctgt agaacagcaa gatggattta tgtctgacct
    33661 cggcgaagca gtaaaagaaa ctggtcgcgg actggtgcag gctggtgtaa atgtggcaaa
    33721 tataccagca tcagttgccg atgctgtaac aagcgcggcg gcatgggctg gcggtaaact
    33781 cggtattggc gatggaacat atcaaccagc gccacgagta acaactaagg gattagagca
    33841 ggcgtttgga cttcagcaag gtgctctgac tccacaaacg acagaaggta gggtgtttgc
    33901 ggaggcattg ccttacctgg ctactgttgg cgttggcggc gcttcaactc aggcgccaac
    33961 acttgctggt cgaattactc aaggcgcagc ccgtcttctg gcagaaaacg cagtcggatc
    34021 gctcgccgca aacagtgaga aaaatgatgc gggaaaactg gcaacagata taggtgttgg
    34081 catgctaaca ggtggtgctg ttaatactgt tgcaaaaggg cttgagcgtg gaataactgc
    34141 ctttaaaggt gatattgcac cagaagtggc gaagaaaatt gccacatcag aatcgatggg
    34201 cgtgacacca atgacatctg atgtaatccc gccgaaaaat gctttcactc gtggtcttac
    34261 tcagggtgct gagggggctt tgctgggaac aggctcaaag cgagctgagc agtatgcaaa
    34321 aagaagtcag ctagttaaaa aacagcttga gaaatatggt gaatatagcc catcagttgt
    34381 tgttgacgat ctgtatggct ctctgaagtc aaggaaggat tcggccggaa gcgttattga
    34441 agacattaca gccaaaatgg gagacacacc agttgacaca tcaaaatcta ttaaggttat
    34501 cgacaacgta cttaccagag ctaataggct tgggaaagtg gcaaataagg atttgattcg
    34561 cgggttatcc gatttgcgag aagaacttgc taaaccagat atagattttg gtctattgag
    34621 agagttgcgg tcagccttga gggaaagtat tcagggagat gccatggttt ttcctaatag
    34681 cgcgaaagcc gcaactgatg ccgtggagag ggcaatgggg gcggatttga ggaataacgc
    34741 agcgcggtat ttgggggctg gagaggccgc cagatacgtc aaagcaaact ctgactactc
    34801 caacgtcttc aataaggttc ttaataaaag gattgcgaat aatctcaaca aagctaaaaa
    34861 agagtttact ccagagctaa taaacagcgt tgtattcagc agaaaaccat cagatattaa
    34921 gaggatatgg ccagctctta gtgaagatgg aaagaacgct atgcgtgctg cttatatcag
    34981 caagattgca gaaaaagcag gagactcgcc aacaaaattt cttaccgagt taaataagtt
    35041 gaagtcgcaa tctgacggtc agatctataa cacgatattc agtgggagac acatgaaaga
    35101 gcttgatgct cttcatgaag ttctacagca aacagcaagg tcggacgccg caaatgtagt
    35161 aacacagaca ggacaatcgc aagctaacag gattagaacg attggcgcaa ctgcaaccct
    35221 tggtgtatca ctgggacttg aggctggttt cggtgcaatc atgcgcttgt atgagtccaa
    35281 agcagcaagg aatgctctct tacgtctggc aaacactaaa gctggaacgc cagcttatga
    35341 aagagcgtta agcaaagctg caaatgccat cagaccgctg cttgccactg aggcaacaca
    35401 gcagtgacta aatgccatgg atggttattt acctagcaca tgaaacaatg tttgctttaa
    35461 ttccacccat acaattatga ccactataga cagacaaaag aaactgaatg cattggcatc
    35521 acgatcgaaa ccttctccgg cactaaatcc gtaaaaggtc ataaaaaata taaaaattgc
    35581 gcactttgca acgtttacaa attttttctt cacaccaacc tccttagttt tgtgcaggat
    35641 accatgaaaa aagttaacat tggaaacgta ccaaagatgc tcgttccgct ctttgagagc
    35701 ggtacaattg tgttttgtag agactttcca gaatggcaac gcctgcatca aaaacttggc
    35761 gttgacgtgc atgactctga caccaacgga gcgtctcata caatgagtag cgagaatggt
    35821 gttttgcatg tgataggcgt gttcaatggc aaactatcta ctattgccca tgagtgcgct
    35881 cacatggcat tcgatatctg ctcaagggta ggtgttgatg ttgaaccagg aagagccaac
    35941 gagacttact gctacttaat gagcaggctt gttgagttct gcgagcgaca tatcaaaaag
    36001 ccggagtgac ccggcttgat tattactttt tttggtatgt taagaatggc aagtcgccag
    36061 tgtaccccaa gcggaaaagc tccaattgct tatctcttat ggataaaaaa taatcgtttc
    36121 ttgccttttt aagccacaac cagccgatca tcaagatgaa taatattgat attattggca
    36181 tcggggagat caacaccact ccgatgaata atgacgctag cagcaccgat aaaatagcag
    36241 aaatcatact aatctcccac taaggtaaca atatgactat agaagaacgt ctgaacaata
    36301 ttgagttgaa ccaaaccctg cttgaccagc gactttcaga tcttgagctt aaagatttgg
    36361 atgcgcaaat atcagaagca gaagccaagc tctccagctt aaaccaccgt aagaagcaaa
    36421 tccgcaacag aattactcag ggacgcggaa gctgttgagg tgggatgcta ggtctctatc
    36481 gttaaaatca aggctgctaa tcatttcatt gtaaatggcg tttttatctt ccattggcag
    36541 ccttgagtaa accagacaca gagcatattt cagggagttt agctctttct ctagctcttc
    36601 cttgcttgac gtttttgact taataaactg ttttttattc attttgcatc cttaccatac
    36661 atgattttca gcgtctcaac atcgtgttca agatctatca atcgtgatgc tatagttgca
    36721 aggtctagtg cttgaatgtg tttatttttt tctgtccacg cttcaagtgc cgcgaccatc
    36781 tcagcattta atgaacgaga attagcctca gccagttcaa taagacgttc ctttatctct
    36841 acaggaagcc tcagattcac ttgagggttt ttgtacttac gatcagacat cggcgcatcc
    36901 tgaataattt tttaccacag gatatgtagg tatctattga ctatcaatgc gtacctaaat
    36961 actatgtatg cgtaccacat acaacggagg atacaatgaa ggttaaaaca ctgcgtatgc
    37021 cagagaagct agaaaaaatt cttgaagaaa aagcaaaaga agagtgtcgc tcattcagtg
    37081 cagaagtaat taaacgggtg ctggacagcc tgaagaggga gggggtaaca gtgtgatttg
    37141 cttggatgca gtagtcaaag agattgtgct taccattttg agaccaaatt ttgttcatgg
    37201 cgaatcattt ggcgcgaacg aaaaattgag aagagaaaga gtattcagtg gcaagcgcac
    37261 gttttcagaa gtgatgcttg atgcggcaaa gaaatcaaaa cagtgaagcc ccaactgcgg
    37321 gaacagtcag ggcttcggta tcgtaaaacc acgcatagga attaacgaca tgaaaagtat
    37381 agcaacagca gtatctacta tcaatgtacc attccacggc gcagagcttt atgttgtcaa
    37441 tcacaacggc gaaccgtata ccccaatgaa acctatcgtt gaaggcatgg gtatggattg
    37501 ggcttcacag tttacaaagt taaaacaaag atttgctaaa ggtattgtgg aaatcgcaat
    37561 accttcagtt ggtggtgtgc agaccatgat ttgccttgct ttgcgtaaac tgaatggctg
    37621 gctgcaaacc atcagtccta acaaagtccg ccccgaaatc cgcgacaagg taatccagta
    37681 tcaggaagag tgtgacgatg tgctatacga gtactggact aaaggtcatg tagttaaccc
    37741 acgcaaagct aaaaaggcgt tgccgggtaa aatcaccact gaacagcagg aagccattaa
    37801 acaactcgtc atgagtcgcg gtcagtctct gccaaaagaa aaacaggcta aggcgatgat
    37861 caccatgtgg tcgtcactga aatcccattt tggatgttcg tacaaagaaa tcagtgagga
    37921 gcagtttacc gaagcactgt cacttgcagc tcgagttccg cttgaaggcg agttcattgg
    37981 caagcaagaa gagttaccag cgccaaaact ggatattaac ttcccgattt catggttttc
    38041 tgaaaatgca ccttacgcca ttatcaggca gcaatgcggt gatactgttg ctcttgaccg
    38101 cagcgcactc gttgaatgca gtccggcgta taaactaatc gatattctga caaaagcggg
    38161 gtatgacgtt tcagcagtaa gagccgagct aaaagcactt cgccatttaa tggctgagca
    38221 aagctgggca cttaaagaga tagcttcatt cgcagcgata cgtgatagag cgtgccactc
    38281 aattaaactt taactacctg cacgaaaagt gcaggtcaat aaccaacgca acgacccagc
    38341 ttcggctggg tttttttatg cccaaaattc accgtagcca cgctgcggcg attccttgca
    38401 tctggagcaa attaaatgac agacattaca gccaatgtga ttgtatctat gccttcgcaa
    38461 ctcttcacta tggcgcgttc ttttaaagcg gttgccaatg gcaaaattta tatcggaaaa
    38521 attgacactg acccggtaaa tcctgaaaac cagattcagg tttatgtaga gaacgaagac
    38581 ggttctcaca ttcctgtttc gcaaccaata atcattaacg ctgctggata tccggtatat
    38641 aacggacaga ttgccaaatt cgttaccgtg caaggccatt ctatggctgt ttatgatgcg
    38701 tatggtgcac agcagttcta ttttcccaat gtgctgaagt atgaccctga tcaactacgg
    38761 cagcaattag aagacccaga tggagcgaat aaatacccaa aacttcagat agcaagatgg
    38821 agagacagtt atgatgtaag aggttggggg gctattggtg atggtgttca tgatgataca
    38881 tcagctctat cagaattact ttctgttgca acaggtggtg aaaagataga tgggcgaggg
    38941 cttactttta aagtatcaac tcttccagat gtcagtcgat ttaaaaatgc tcgtttttta
    39001 tttgagagaa taccgggtca gcctcttttt tatgcttctg aagattttat ccagggagag
    39061 ttatttaaaa ttacagatac accgtggtac aacgcctgga cgcaggataa aacgtttgta
    39121 tatgacaatg tcatctatgc gccttttatg gctggagacc gccatggtgt aaataacctc
    39181 catgttgcat gggttcgctc aggagatgac gggaagacct ggacaacgcc ggaatggctt
    39241 acagatttac atgaaaacta tcccacagtt aactatcact gcatgagtat gggggttgtc
    39301 agaaatcgcc tttttgctgt aattgagacg cggaccgtga gcggaaataa actgcaggtt
    39361 gcagagttgt gggatcgccc aatgagtcgc agccttcgcg tttatggtgg tataacgaaa
    39421 gcagcaaatc agcaagtcgc ttatattcgc attactgatc acggattatt tgctggtgat
    39481 tttgtcaact tctcaaactc tggtgttaca ggtgttaccg ggaatatgac ggtgactact
    39541 gtcattgata aaaatacttt tacagttacg acgcaaaata cccaggatgt ggatcagaat
    39601 aacgagggta gatactggag ttttggtaca tcatttcact cgtcaccatg gagaaaaacc
    39661 agtcttggaa ctattccttc ttttgttgac ggaagcactc ctgttactga gattcacagt
    39721 tttgcgacga ttagcgataa cagttttgct gttggctacc ataatggtga tattggtcca
    39781 cgcgagcttg ggatactcta tttctctgat gctttcggtt ctcctggtag ctttgttcgc
    39841 agacgcatac ctgcagaata tgaggcgaat gcatctgagc catgtgtaaa atattatgat
    39901 ggcattctgt atctgacgac cagggggaca ttaagtactc aacccggtag ttcattgcac
    39961 agaagctctg atttaggtac atcatggaat tctcttcgct tcccaaataa tgttcatcac
    40021 tcaaaccttc cttttgccaa agttggcgat gagctgatta tttttggcag tgagcgcgca
    40081 tttggtgagt gggaaggagg agaacctgat aaccgttatg caggaaatta tccaagaaca
    40141 tttatgacca gagttaacgt caatgagtgg agtctggata atgtagagtg ggttaatgtt
    40201 actgatcaga tttatcaggg cggaatagtt aactctgcgg ttggtgttgg ttcagtttgt
    40261 atcaaagaca actggctgta ctacattttc ggtggggaag actttctaaa cccatggagc
    40321 ataggggata acaacagaaa atatccttat gttcacgatg gtcacccggc tgatttgtat
    40381 tgtttcaggg tgaaaattaa acaggaagaa tttgtttcaa gggattttgt ctacggagcc
    40441 actcctaaca gaacgcttcc tacttttatg tcgacgtcag gcgtgaggac ggttcctgta
    40501 cccgttgatt tcacagatga tgttgccgtc cagtcactga ctgtccatgc aggtacatca
    40561 ggacaagttc gcgcggaagt caaacttgag ggtaattacg ccattattgc gaagaaagta
    40621 ccgtctgatg atgttaccgc tcagagatta atcgttagcg gaggtgaaac aacgtcttca
    40681 gcagatggtg caatgataac gttgcatggt tccggaagca gtactccacg tcgcgcggta
    40741 tataacgcac tcgaacatct ttttgagaac ggagatgtta aaccttatct tgataatgta
    40801 aatgctcttg gtggtccggg aaacaggttc tcgatagttt atcttggctc caatcctgtg
    40861 gttaccagtg acggaacatt aaagacagag ccggtctctc ctgacgaaac attgctggat
    40921 gcctggggtg acgtcaggta tatcgcttat aaatggctga acgctgtcgc tataaagggg
    40981 gaagaagggg cgaggataca tcatggtgta atcgcgcagc aacttcgtga tgttcttatt
    41041 tctcacggac tcatggaaga agaaagcaca acatgccgct atgcctttct ttgctatgac
    41101 gattatcccg cagtatatga tgacgtcatt actggccaaa gggaaatgcc gctgactgat
    41161 aatgacggga gcatcattgt tgatgaggat gataatccag tgatggtaat ggaagacatc
    41221 attgagcgcg ttgaaataac gccagcagga tctagatggg gggtcagacc tgatctctta
    41281 ttctatatcg aggcagcatg gcagcgcaga gaaatagaaa gaataaaagc taggttagac
    41341 ttaatagaag ggaagcacta aatgtttcgt tggcgtcaaa aatatgaact gatcacataa
    41401 agaagtaagt ac
//
LOCUS       DAJOTB010000023        40798 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJOTB010000023 DAJOTB010000000
VERSION     DAJOTB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.023"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            108..872
                     /locus_tag="OHN28_001264"
     CDS             108..872
                     /locus_tag="OHN28_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296698.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(911..1837)
                     /locus_tag="OHN28_001265"
     CDS             complement(911..1837)
                     /locus_tag="OHN28_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2296699.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1927..2925
                     /locus_tag="OHN28_001266"
     CDS             1927..2925
                     /locus_tag="OHN28_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCQ2296700.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2922..3140)
                     /locus_tag="OHN28_001267"
     CDS             complement(2922..3140)
                     /locus_tag="OHN28_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCQ2296701.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(3142..5157)
                     /gene="ligA"
                     /locus_tag="OHN28_001268"
     CDS             complement(3142..5157)
                     /gene="ligA"
                     /locus_tag="OHN28_001268"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCQ2296702.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5228..6226)
                     /gene="zipA"
                     /locus_tag="OHN28_001269"
     CDS             complement(5228..6226)
                     /gene="zipA"
                     /locus_tag="OHN28_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCQ2296703.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6456..7217
                     /gene="cysZ"
                     /locus_tag="OHN28_001270"
     CDS             6456..7217
                     /gene="cysZ"
                     /locus_tag="OHN28_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCQ2296704.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7402..8373
                     /gene="cysK"
                     /locus_tag="OHN28_001271"
     CDS             7402..8373
                     /gene="cysK"
                     /locus_tag="OHN28_001271"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCQ2296705.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8757..9014
                     /gene="ptsH"
                     /locus_tag="OHN28_001272"
     CDS             8757..9014
                     /gene="ptsH"
                     /locus_tag="OHN28_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCQ2296706.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9059..10786
                     /gene="ptsI"
                     /locus_tag="OHN28_001273"
     CDS             9059..10786
                     /gene="ptsI"
                     /locus_tag="OHN28_001273"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCQ2296707.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10827..11336
                     /gene="crr"
                     /locus_tag="OHN28_001274"
     CDS             10827..11336
                     /gene="crr"
                     /locus_tag="OHN28_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCQ2296708.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11379..12230)
                     /gene="pdxK"
                     /locus_tag="OHN28_001275"
                     /gene_synonym="thiJ"
     CDS             complement(11379..12230)
                     /gene="pdxK"
                     /locus_tag="OHN28_001275"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCQ2296709.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12335..12703
                     /locus_tag="OHN28_001276"
     CDS             12335..12703
                     /locus_tag="OHN28_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCQ2296710.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12706..13617)
                     /gene="cysM"
                     /locus_tag="OHN28_001277"
     CDS             complement(12706..13617)
                     /gene="cysM"
                     /locus_tag="OHN28_001277"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCQ2296711.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13752..14849)
                     /gene="cysA"
                     /locus_tag="OHN28_001278"
     CDS             complement(13752..14849)
                     /gene="cysA"
                     /locus_tag="OHN28_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCQ2296712.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14839..15714)
                     /gene="cysW"
                     /locus_tag="OHN28_001279"
     CDS             complement(14839..15714)
                     /gene="cysW"
                     /locus_tag="OHN28_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCQ2296713.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15714..16547)
                     /gene="cysT"
                     /locus_tag="OHN28_001280"
     CDS             complement(15714..16547)
                     /gene="cysT"
                     /locus_tag="OHN28_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCQ2296714.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16547..17563)
                     /gene="cysP"
                     /locus_tag="OHN28_001281"
     CDS             complement(16547..17563)
                     /gene="cysP"
                     /locus_tag="OHN28_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCQ2296715.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17721..18512)
                     /gene="ucpA"
                     /locus_tag="OHN28_001282"
     CDS             complement(17721..18512)
                     /gene="ucpA"
                     /locus_tag="OHN28_001282"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCQ2296716.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18792..19688
                     /gene="murQ"
                     /locus_tag="OHN28_001283"
     CDS             18792..19688
                     /gene="murQ"
                     /locus_tag="OHN28_001283"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCQ2296717.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            19692..21116
                     /gene="murP"
                     /locus_tag="OHN28_001284"
     CDS             19692..21116
                     /gene="murP"
                     /locus_tag="OHN28_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCQ2296718.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(21294..22193)
                     /gene="yfeX"
                     /locus_tag="OHN28_001285"
     CDS             complement(21294..22193)
                     /gene="yfeX"
                     /locus_tag="OHN28_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCQ2296719.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22289..22864)
                     /locus_tag="OHN28_001286"
     CDS             complement(22289..22864)
                     /locus_tag="OHN28_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCQ2296720.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22925..23374)
                     /locus_tag="OHN28_001287"
     CDS             complement(22925..23374)
                     /locus_tag="OHN28_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCQ2296721.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23361..23786)
                     /locus_tag="OHN28_001288"
     CDS             complement(23361..23786)
                     /locus_tag="OHN28_001288"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCQ2296722.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24000..24869
                     /gene="amiA"
                     /locus_tag="OHN28_001289"
     CDS             24000..24869
                     /gene="amiA"
                     /locus_tag="OHN28_001289"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCQ2296723.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24873..25772
                     /gene="hemF"
                     /locus_tag="OHN28_001290"
     CDS             24873..25772
                     /gene="hemF"
                     /locus_tag="OHN28_001290"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCQ2296724.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25778..26830)
                     /gene="eutR"
                     /locus_tag="OHN28_001291"
     CDS             complement(25778..26830)
                     /gene="eutR"
                     /locus_tag="OHN28_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCQ2296725.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26876..27376)
                     /gene="eutK"
                     /locus_tag="OHN28_001292"
     CDS             complement(26876..27376)
                     /gene="eutK"
                     /locus_tag="OHN28_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCQ2296726.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27389..28048)
                     /gene="eutL"
                     /locus_tag="OHN28_001293"
     CDS             complement(27389..28048)
                     /gene="eutL"
                     /locus_tag="OHN28_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCQ2296727.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28058..28945)
                     /gene="eutC"
                     /locus_tag="OHN28_001294"
     CDS             complement(28058..28945)
                     /gene="eutC"
                     /locus_tag="OHN28_001294"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCQ2296728.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSENLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(28966..30327)
                     /gene="eutB"
                     /locus_tag="OHN28_001295"
     CDS             complement(28966..30327)
                     /gene="eutB"
                     /locus_tag="OHN28_001295"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCQ2296729.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30339..31742)
                     /gene="eutA"
                     /locus_tag="OHN28_001296"
     CDS             complement(30339..31742)
                     /gene="eutA"
                     /locus_tag="OHN28_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCQ2296730.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31739..32965)
                     /gene="eutH"
                     /locus_tag="OHN28_001297"
     CDS             complement(31739..32965)
                     /gene="eutH"
                     /locus_tag="OHN28_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCQ2296731.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(33182..34369)
                     /gene="eutG"
                     /locus_tag="OHN28_001298"
     CDS             complement(33182..34369)
                     /gene="eutG"
                     /locus_tag="OHN28_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCQ2296732.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMPETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34359..35195)
                     /gene="eutJ"
                     /locus_tag="OHN28_001299"
     CDS             complement(34359..35195)
                     /gene="eutJ"
                     /locus_tag="OHN28_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCQ2296733.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(35206..36609)
                     /locus_tag="OHN28_001300"
     CDS             complement(35206..36609)
                     /locus_tag="OHN28_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCQ2296734.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36621..36908)
                     /gene="eutN"
                     /locus_tag="OHN28_001301"
     CDS             complement(36621..36908)
                     /gene="eutN"
                     /locus_tag="OHN28_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCQ2296735.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(37015..37308)
                     /gene="eutM"
                     /locus_tag="OHN28_001302"
     CDS             complement(37015..37308)
                     /gene="eutM"
                     /locus_tag="OHN28_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCQ2296736.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37347..38363)
                     /gene="pta"
                     /locus_tag="OHN28_001303"
     CDS             complement(37347..38363)
                     /gene="pta"
                     /locus_tag="OHN28_001303"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2296737.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(38360..39163)
                     /gene="eutT"
                     /locus_tag="OHN28_001304"
     CDS             complement(38360..39163)
                     /gene="eutT"
                     /locus_tag="OHN28_001304"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCQ2296738.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(39160..39861)
                     /gene="eutQ"
                     /locus_tag="OHN28_001305"
     CDS             complement(39160..39861)
                     /gene="eutQ"
                     /locus_tag="OHN28_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCQ2296739.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(39836..40315)
                     /gene="eutP"
                     /locus_tag="OHN28_001306"
     CDS             complement(39836..40315)
                     /gene="eutP"
                     /locus_tag="OHN28_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCQ2296740.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40328..40663)
                     /gene="eutS"
                     /locus_tag="OHN28_001307"
     CDS             complement(40328..40663)
                     /gene="eutS"
                     /locus_tag="OHN28_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCQ2296741.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9403 a        11434 c        10440 g         9521 t
ORIGIN      
        1 aaaactgtat tggtactctc ctttcccttg cattaacaat atgccgcttt taatcaacct
       61 ttttattaag caatcaacta ataacgatcg gcatagggaa ttatgctatg aaaaaacatc
      121 tttttactct gacactctct tctgtattgg cgataccagc agtatcccat gcagaattta
      181 aaggcggttt tgctgatata ggtattcatt atcttgactg gactagcaga accactgaga
      241 aatcgtcgac caaatcacac aaagatgatt ttggatatct ggaactggaa ggcggcgcta
      301 actttagttg gggagaaatg tacgggttct tcgactggga aaacttttat aatgatcgtc
      361 atgataaacc gggtagcgag cagcgttata catttaaaaa taccaaccgt atttacctgg
      421 gtgacaccgg atttaatctc tacttgcatg cgtatggcac ctacggttct gcgaatcgtg
      481 tgaatttcca tgacgatatg ttcctttacg ggatcggcta caatttcacc ggcagcggtt
      541 ggtggttcaa accgttcttt gcaaaacgct atacagacca aacctattat accggcgata
      601 acggttatgt cgccggttgg gttgcaggtt acagctttat gctgggcagt gagaaattca
      661 ccctcaccaa ctggaacgag tacgagtttg atcgtgatgc cacctacgcg gcaggtaatg
      721 gcggcaaaga aggtctgaac ggtgccgtcg cactttggtg gaatgcaact tcacacatca
      781 ccacagggat tcagtatcgt tatgcggatg acaaactggg cgaagatttt taccaggatg
      841 cgataatcta ctccatcaaa tttaatttct gattgattac tgccggagct cccggcagta
      901 tgaattcact ctatatctga tacaacggat ccccttcccg cccagccatt agcgcattgc
      961 cctggtctcg tactacgtcc cataacgctt cagcggcggt ggaaagtgaa cggttcttcc
     1021 tgcgcaccag catcaactgg cgttcaacca ctggcgtaat gcgctttacc accagtgggc
     1081 taccttcagg caatggtagc gccagtgcgg gaagaatact gatgccgatg cctgcggcta
     1141 ccatcgggaa cagcgtcgcc ggatgtccaa tctcctgcac aatattcgcc tgaataccgt
     1201 tgcgcgccag cgctgcgtca atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca
     1261 atttcgctcc ctgtaatgcc tgccagggga cataatcttc cacggccaaa gcgctatcgc
     1321 gatggcaaag cagaaagaaa ggctcggaaa gaatcgcttc acattgcaga tcgcccactg
     1381 ggccaggatc gatgacgatg ccaaaatcga catctccctg gcgaatactt tccatcaccc
     1441 actgctgcgg acgatcgtgc aggacaaact gaatatctgg atagcggcga tggctttcag
     1501 caatgcattg cggaataaga tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc
     1561 cgctcagttg ttgccccata cgcccggtat cgcgcaacgt gctgttaagt tcatccagca
     1621 gtcgctcaag acgcaaagcc aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg
     1681 ttctgtccag caggcgaaca ccagtatgat tttccagctc cttcacactg tgactcactg
     1741 ccgactggct caggccgata cgctctcctg cacgactaaa acttttctcc tgcgctactg
     1801 tgacgaaaac ctttaattgc tttaaagaat aattcatctg tttttttcat gaatggatgc
     1861 aataaatcaa ttttatttct caaactggaa gacgcacaat agaaccatcg atcatctgga
     1921 gtctttatga aactttttcg tatcctcgat cctttcacct taaccctgat cacggtggtg
     1981 ttgctggcct ctttctttcc ggccagaggc gatttcgtcc ccttctttga aaatctgacc
     2041 accgcagcga tagccctgct gttttttatg cacggcgcga agttatcgcg tgaggcgatt
     2101 attgctggcg gtggccactg gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg
     2161 tttccgattc tgggcgtgct gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc
     2221 tactccggtt ttctctactt gtgcattctc cccgcgaccg tgcagtctgc aatcgccttc
     2281 acgtcaatgg cgggcggtaa cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg
     2341 ctggggattt tcctttcacc attgctggtt ggtctggtga tgaatgttca cggtgcaggg
     2401 ggcagccttg agcaggtcgg taaaattatg ctgcaactgt tgctgccatt tgtgttgggg
     2461 catctttccc ggccgtggat tggtgactgg gtgtcgcgta ataaaaaatg gattgcgaaa
     2521 actgaccaga cgtccattct gttggtagtt tatacggcat tcagcgaagc cgtcgttaac
     2581 ggtatctggc acaaagttgg ctggggatca ttgctgttta tcgtggtggt cagttgcgtt
     2641 cttctggcta tcgtgattgt agttaacgtc tttatggcac gccgactggg cttcaataag
     2701 gcagatgaaa ttactatcgt cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg
     2761 atggcaaaca ttctgttccc cacatcggtg atcggtatga tggtgctgcc cctgatgatt
     2821 ttccatcaga tccaattgat ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc
     2881 gaacagttac aggcgcagca ggaaagcagc gccgataaag cttaaagcgg acgtttcagg
     2941 ggctggacca gttgcgtcag cccctcgatt ttaatcagca gcgtgatttg cattagctca
     3001 ccgagcttcc ctgccgggaa ctcatctttg cgggcaaacc acagcagata ctcctccggc
     3061 aggtcgatta agcgacgccc tttgtacttg ccaaacggca ttatcgtatt ggctatttca
     3121 atcagctgct ctttttccat ctcagctacc cagcaaacgc agcatttccg tttcgtcgat
     3181 gacttcaata cccagttcct gcgccttcgc cagtttagat cctgcagctt cacccgctat
     3241 caccagatcg gttttcttcg acacgctgcc cgcgactttc gcccccagtt cgaccagtcg
     3301 agctttagcg tcatcacgcg acatctggct taagctgccc gtaagcacca cggttttacc
     3361 agcaaacgga ctgtcaatct cttccgcatt gataacgatc ggctcaggcc agtgaacacc
     3421 ttccgccaac agctcgctga tgacattgcg gttgctttct tcggcaaaga agttgtgaac
     3481 gtgggaggca acgacaattc caacatcagg caccttttgc agctcttcaa tcgacgcggc
     3541 ttccagcgct tccagcgtgc cgaaatatgc cgccagacct gctgcggtag cctcgccgac
     3601 ttcacggatg ccaagtgcat agaggaagcg ggcaaaggtg gtttctttcg ctttttccag
     3661 cgcgttaacc acgttttgtg ccgatttcgg ccccatacgc tccagtccgg tcagtttgcc
     3721 tgcggtgagt ttgaacagat ccgccggagt gtggacatat tctttttcaa ccagttgatc
     3781 gatgattttg tcgcccattc cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag
     3841 agactcttta cgctgcgcac cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc
     3901 ttccacacgc tcgacgtcag aaccacatac cggacaatgc gtcgggaata caacctcacg
     3961 ggtatcttcc gggcgttcag aaagcacgac gttaaccacc tgcgggatca cgtcgccagc
     4021 gcggcgaata accactttat cgccaatgcg taaaccaaga cgttcgattt cgtcagcatt
     4081 atgcagtgta gcgttactca ccaacacgcc agcaacatgg acaggttcca gacgcgcaac
     4141 aggcgtaatc gcgccagtac gcccaacctg aaactcgacg tcacgcacaa aggtcatctg
     4201 ttcctgcgcc gggaatttaa acgctaccgc ccagcgcggg gcacgcgcga caaagccaag
     4261 ctgctcctgc tgtgccagtg agttgacctt aatcaccacg ccgtcgatat caaagcccag
     4321 cgtcggacgg tctttttcta ctttgtgata aaacgccagc acttcttccg ccgattcaca
     4381 aagtgttacc cgatcactga caggtaatcc ccatgcttta aattgcatta agcgtcccaa
     4441 atgggtatcc ggcaattcac cgccttccag aacaccaacg ccgtagcaga agaaagtgag
     4501 cggtcgcttc gctgtaatac gcggatcaag ctgacgcagt gaaccagctg ccgcattacg
     4561 tgggttagca aacactttcc cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc
     4621 cgcctgcggc aggaacactt caccacgcac ttccagacgc gccgggatat tctcaccgtg
     4681 tagcttcagc ggaatggcgc gaatagtacg cacattagac gtgatatctt ccccggtagt
     4741 gccatcgcca cgagtcgcgg cactgactaa aacgccattt tcatacagaa tactaacggc
     4801 aagaccatcc agcttcagct cacagcacca ggtgactttc tcgttgcttt tcagacggtc
     4861 ctgcacacgt ttgttgaaag caagaaagct ttcttcatca aaaacgttat ccagcgacag
     4921 cattggcact tcatggcgga tctggctgaa agccgccagc ggcgctgcac ctacacgttg
     4981 ggtaggcgaa tcaggcgtaa tcagttctgg atgtttggtt tccagctcgc gcagttcgcg
     5041 catcagcctg tcgtattcag cgtcgggaat ttccggcgca tccatcacat gataaagata
     5101 ttcatgatgg cgaagcgtcg ttcgcagttc tgtcagttgt tgttcgattg attccatatc
     5161 gcaccatcaa tgctaaaaac ccccgacaag cgggggttcg aaaaggagtt aatttgcctt
     5221 aagtgtatca ggcgttggcg tctttgactt cgcggatgat gtcctggtac tcgcgcaatt
     5281 tctgcggagt catcatacgg cgctgatcgt caagcactac accacccact tcatcggcaa
     5341 tatgctgcgc agattgcagc atcagcttga agttctgcag ctcgtcaccg taagacggta
     5401 cctgcataaa gatagtgaca cccggagtag tgaaatcctt catttcagga tcaaaggttc
     5461 ccggtttcac catattcgcc aggctgaata acgccgggcc gctgccatcc gggctaagat
     5521 gacgatggta aatgttcata tcaccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa
     5581 gcagttcacc gtttaactcg ctaccgtgat gcgccgcgac gttcatgata atcaccgctt
     5641 ctttgcgctt cggtttatcc ataactggag ccggttccgc aacaggctca ggctgtggtg
     5701 ccgctacggg ttctgcaggc tggaaagcct gttgtgccgg ttgcggtgct gaatgcactg
     5761 gctgcggcgc tgacgcgacc tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg
     5821 gctgataggc aggctgctgc acgggttgct gcaccggctg cgttggacgc ggagcatgtt
     5881 gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat
     5941 aaggcggttg gtactggtgt tgcggcgacg gacgagcaac ctcatgctcc tgggcgttag
     6001 ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat
     6061 cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga
     6121 acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg
     6181 cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg
     6241 ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc
     6301 aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct
     6361 gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt
     6421 ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc
     6481 cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc
     6541 ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt
     6601 ggctctttac gcaactcgat gtctggatcc cgactctcat gagttatgtt ccggactggt
     6661 tacaatggct gagctatctg ttgtggcccc tggctgtcat ctctgtgctg ttagtgtttg
     6721 gctatttctt ctcaacgatt gctaactgga ttgctgctcc gttcaacggt ttattggctg
     6781 aacaactgga agcaagactg actggcgcta caccgccaga taccggaatt ttcggtatca
     6841 tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgcctgg tatctgccgc
     6901 gcgcaattgt attgctaatt ctttacttca tccctggcat tgggcaaacc gtcgcgccgg
     6961 tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgtgat taccccttcg
     7021 ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca
     7081 atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca
     7141 ttatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt tgattgctat cgcgataaac
     7201 acgccatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg
     7261 tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg catatagata
     7321 tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt
     7381 tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt
     7441 cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa
     7501 tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc
     7561 gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccaaccag cggtaatacc
     7621 gggattgcac tggcctatgt agctgccgct cgcggttaca aacttaccct gaccatgcca
     7681 gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg
     7741 accgaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc
     7801 aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcat
     7861 gaaaaaacca ccggtccgga gatctgggaa gataccgacg gtcaggttga tgtatttatt
     7921 gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa
     7981 ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag
     8041 gcgctggcag gtgaagagat taaacctggt ccgcataaaa ttcagggtat tggcgctggt
     8101 tttatcccgg ctaaccttga tctcaagctg gtcgacaaag tcattggcat caccaatgaa
     8161 gaagccattt ctaccgcacg tcgtctgatg gaagaagaag gcattcttgc tggtatctct
     8221 tctggagcag ctgttgctgc ggcgttgaaa ctacaagaag atgaaagctt taccaataag
     8281 aatattgtgg ttattctgcc gtcatcgggt gagcgttatt taagcactgc attgtttgcc
     8341 gatctcttca ctgagaaaga attgcaacag taatgccagc ttgtaaaaaa tgcgtaaaaa
     8401 agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga
     8461 ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg
     8521 ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg
     8581 cataatgttt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat
     8641 tacgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga
     8701 accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt
     8761 tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt
     8821 ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg
     8881 ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga
     8941 ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg
     9001 cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat
     9061 gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga
     9121 agacgaaatt atcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga
     9181 acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc
     9241 tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga
     9301 agatgaggag ctggagcagg aaatcatagc cctgattaaa gataagcaca tgacagctga
     9361 cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga
     9421 atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat
     9481 cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc
     9541 tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac
     9601 cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc
     9661 tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga
     9721 tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc
     9781 tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc
     9841 tattacgctg gacggtcacc aggtagaagt gtgcgctaac attggtacgg ttcgtgacgt
     9901 tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt
     9961 catggaccgc gacgcgttgc ccactgaaga agaacagttt gctgcttaca aagcagtggc
    10021 tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga
    10081 gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg
    10141 tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgtgctatcc tgcgtgcctc
    10201 tgctttcggt aaattgcgca tcatgttccc gatgatcatc tctgttgaag aagtgcgtgc
    10261 actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga
    10321 cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca
    10381 tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct
    10441 ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt
    10501 gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat
    10561 gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga
    10621 cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa
    10681 cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt
    10741 aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg
    10801 cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt
    10861 ccgacgacaa gaaggatacc ggaactattg agatcattgc tccgctctct ggcgagatcg
    10921 tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggcattg
    10981 ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggcaccatt ggtaaaatct
    11041 ttgaaaccaa ccacgcgttc tctatcgaat ctgatagcgg cgttgagctg ttcgtccact
    11101 tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc
    11161 agcgcgtgaa agttggtgat actgtcattg aatttgatct gccgctgctg gaagagaaag
    11221 ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca
    11281 aactgtccgg tagcgtaacc gtaggtgaaa ccccggttat ccgcatcaag aagtaattct
    11341 tgccgcagtg aaaaatggcg cccatcggcg ccattttttt atgcttcagc cagaggcggc
    11401 aaaatcaatt catcgctctc atgctgctgg gtatagcgca tcacttccag tacgcgcaac
    11461 cccgcgcggt gcaccgcatc ggttaacgcc ttccctttca gcaaaccact gataagctga
    11521 gcacaaaaca ggtcgccagt ccctttcagg tcagttttaa cccgggaatg ggaaatgaca
    11581 ttcacgctgt cggcactgac caccacaacc tgcatctcct gattttcttc attgccggag
    11641 gcgctggtaa tcaccaccca ctttaatgtg tctgaaagca gactttttgc ggcagcaatg
    11701 gcactgtcga gatcgcggca atctttaccg gtcaggattt ccagctcaaa gatattgggg
    11761 gtaattccct gtgccagcgg cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg
    11821 acataaattc cgctatcaat atcgccaatc accggatcga ccatgatcaa taggtcagga
    11881 tggtctttgc gtagcgcagt cagccactcg gcaaggattt tgatttgcga tgccgttccc
    11941 atatagcccg tggttacagc acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc
    12001 aaataaccgc taaaccattc atccggaatc gcaccaccgt agaaagtgtc ataatgcggc
    12061 gtattgctca gcaataccgt cggcacggca aagacattca ggccgttctg ctttatagca
    12121 ggcaccgcaa tgctgttgcc tacgctgccg tacactacct gcgactgcac ggcgacgata
    12181 tccgcctgca gtgccctgct cttatcgtta aacaacaaca aactactcat ttaatttttt
    12241 ctccttgccg atgatcctca tcgtattcca gccgaaactt tacctgattc tggcagtcaa
    12301 atcggctatt acaaaacaag gataaggtaa ttcagtgaag aaaatcattt gtctggtcgt
    12361 tacgctgcta atgacactcc ccgcttatgc gaagttaacg gcccatgaag aagcccgtat
    12421 caacgccatg ctggagggat tagcacagaa aaaggatttg atatttgtgc gcaacggtga
    12481 tgaacatacc tgcgatgaag cggtttctca tctgcgcctg aagctgggca atacccgtaa
    12541 tcgcattgat acagccgagc agtttattga caaggtggcc tcgtcgtcat cgattaccgg
    12601 gaagccgtat atcgtgaaga tgcccggtaa gagcgatgag aacgcacagc cttttttaca
    12661 tgcgttaatt gcgcagacgg ataaaacggt gcctgcgcaa taatcttaaa tccccgcccc
    12721 ctggctaaaa tgctcttccc caaacacccc ggtagaaagg tagcgatcgc cacgatcgca
    12781 gatgatcgcc accaccaccg cgccagggtt agcttttgcc acccgcagtg ctccggcaac
    12841 cgcgccgccg gagcttacgc cgcagaaaat cccttcccgc actgccagtt cgcgcatggt
    12901 gttttccgca tcgcgctgat gaatatccag cacctcatcc accagagaag cattgaaaat
    12961 ccccggcaga tattccgcag gccagcggcg aatgccggga atactgctgc cttcttccgg
    13021 ctgcaggcca acaatggtca ccggtttgga ttgttcgcgc ataaagcgtg acacgccggt
    13081 gatagtgccg gttgttccca tgctggagac aaaatgggta atacgcccgc cagtttgctg
    13141 ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg ttatcgggat tattgaactg
    13201 atcgagcaac tttccttcgc cgcgattcgc catctccagc gccagatcgc gtgcgccttc
    13261 catgccctgt tctttggtga ccagaatcag ttctgcaccg taggcacgca tcgccgcacg
    13321 gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt taagcgccgc
    13381 aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgatcagca catcacccgg
    13441 tttaatctcg ccgcgctttt ccgcctcgac aatcatcgaa agcgccgcac ggtcttttac
    13501 cgatcccgcc gggttattgc cttccagttt taaccacact tcactgccgt tatccggcgc
    13561 cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg tactcacgat
    13621 ctctgtccat acgtggtgtt gcctgatgcg acgcttacgc gtcttatcag acctacaggt
    13681 tacaaaacct tgccattaaa aaacccggat cgcgggaagc gcctccgggc gtttaacatt
    13741 cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc
    13801 tcatcgccgt tatacagccg cgcatgttgc agaccaacgt ataaacgatc gccacgctgc
    13861 ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc
    13921 accactaatt gggtgtagtg acctttcggg ctggcttcca gtacctgtac gggtagcggc
    13981 gaatcgaggc tggtacggcg gctgatatcc acttcccacg ggcgcaggaa gagatccacc
    14041 ggcccctgat acgcgggggt gtatcccagc ggccagcgat gtgcgccaac gtggaactgc
    14101 ccgccgcgaa tggttccctg caggcggttt acctcgccca taaactccag cacaaaacga
    14161 gtcgccggtt cacgccatac ctgatccggt gcgtcagctt gttcaatatt gccctgactc
    14221 atcaccacta cacgatcagc tacttcggtc gcctcttcct ggtcgtgggt cacgaacacg
    14281 ctggtgaatt tcagttcttc atgcagttga cgcagccagc gacgcagctc tttacgcacc
    14341 tgcgcatcca gcgcgccaaa cggttcatca agcagcaaaa tttgcggttc cacagccagc
    14401 gcgcgtgcaa gcgccacacg ctgtttctga ccgccggaaa gctgcgccgg ataacgatcc
    14461 gccagatgag caagctgcac catctccagc aattttgtca ctttcgcttt gatcgccgcc
    14521 gcattcgggc gttcacgccg tggcaacacc gtcaggccaa aagcgatatt gtcaaacacc
    14581 gtcatatggc gaaacagcgc gtaatgctgg aacacgaaac cgactttacg gtcccgcgcg
    14641 tgcagacggc tcacgtcggt accgtgaaag cgaatatgcc cactggtttg atgctccagc
    14701 ccggcgataa tgcgcagcag cgtagttttt ccggaaccgg acggcccaag caacgcgacc
    14761 atctgaccag aaggaatatc cagtgagata tcgttcagca cctgggtgcg accaaaagac
    14821 ttcttaatat tggcaatctc aatgctcatg atgttcctcc tgctgcgcgc gtttttcctg
    14881 attttccagg cgccactgca acatactctt taaaaacagg gtgataatcg ccatcagcgt
    14941 caacaacgcg gcggcggtaa aggagccgac ggtgttgtag tcctgctcca gcaattcaat
    15001 ctgtaacggc agcgacaggg tttcgccacg aatcgagccg gaaaccaccg ataccgcgcc
    15061 aaactcgcca atcgcgcggg cgttggtcag caccacgccg taaagcagcg cccagcggat
    15121 gttcggcagc gtgacgcgac ggaacatctg ccagccggac gcgccaagta aaatcgccgc
    15181 ttcgtcttcc tggctgccct gacttaacat taccggtacc agttcgcgca ccacaaacgg
    15241 acacgtcacg aagatggtga ccagcaccat acccggccag gagaacataa tttgcagatt
    15301 atgttcatcc agccagccgc ccagcgggcc gttagagccg tagaacagca gatacaccag
    15361 accagcaacc accggcgaca cggcaaacgg aatgtccagc agcgtcagca atagctggcg
    15421 tccggggaag ttaaagcgcg tcaccagcca ggccagcaga atgccgaaca ccaggtttac
    15481 cggtacggca atcagcgcga tcatcaccgt cagccagatg gcgtgcagca tgtccggatc
    15541 ggccagattt tgtaacaccg gcatcagtcc tttgctgaac gcctgtacga agatataaat
    15601 catcggcacc agcaggatga acgccgatac cagcatcccg atgccaatca gaaaccattt
    15661 gccccagtta atcgggcgcg cgtcataacg cttcaattgt gtcacttccg ccattaatga
    15721 cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag caacagcagc
    15781 gatgccgcga ggatcaccga ggcaatcgcg ctcgccgccg ggtaatcaaa ctcctgtaag
    15841 cgaacaaaga tcatcagcga cgtcacttct gttttccagg cgatattccc ggcgataaaa
    15901 atcaccgcgc caaactcacc gaggctgcgg gtaaacgaca gcgccacacc cgccaccagt
    15961 gccggagata gttccggcag caccactttg cgaaaactct gccagcgtgt tgcgccgagc
    16021 gtttctgccg cctcttcata ttccgggcct aactcttcca gcaccggctg cacggtgcgc
    16081 accacaaacg gaatgctggt aaaggccatc gccaccgcaa tccccagcca ggtataggtg
    16141 actttgatat caaacttcgc cagccattca ccgtaaaaac cgttcacgga aaagagcgag
    16201 gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat cagcgcatca
    16261 agcagcgtgc gacccggaaa gcgatagcgg gttaagatcc acgccatcag cagaccgaaa
    16321 acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg tcactttata ggctgcgacc
    16381 acctgcgggt tggtgatcac ctcccagtac tgcgcccagc tcatctgggc cagttgcatc
    16441 accagcgcgg agagcggcag cagcaaaatc aggcacacaa acagcagact ggtgccgagg
    16501 cttaaggtaa agcccggcag cacgcgtctt gaagaaactg caaacattac ttacgccccg
    16561 ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc acttccggcc
    16621 aggagccaaa tttatcttcc acgcggaaca gctcggtctg cgggaattta tctttcagtt
    16681 tgcccatgac ttccgggtta tttacgcggt agtaatagtc ggtgatgatg gtttgcgcct
    16741 gcgggctgta gagccagttc aggtaggctt ttgccgcttt ttccgtgccg ttggcctgta
    16801 cgtttttatc cacccacgcc accgggaatt ccgccagaat gttggttttc ggaatcacca
    16861 cttcaaagcc ctgcgcttca tactgtttac ggatgttgtt cacttccgac tcgaagctga
    16921 tcagcacatc gcccaggccg cgctcggcaa aagtggtggt cgcgccacga ccgccagtat
    16981 cgaacacttc aacgtttttc aggaactggg tcataaactg ttcggttttg gctttatcgc
    17041 caccgtcggc tttatccgct gcaccccacg cggctaaata ggtgtaacgc gcgttaccgg
    17101 aggttttcgg gttcggaaaa atcagcttca cgtcggagcg caccagatcg ttccagtcgt
    17161 ggatattctt cgggttaccc ttacgcacca ggaagcccat ggtggagtag aacggcgagc
    17221 tgttgtttgg cagacgcgac tgccagtcag ccggaataag cttgcctttg tcgtgcagga
    17281 tctgcacgtc tgtcacctgg ttataagtga caacgtcggc ttttaagccc tgcaaaatcg
    17341 ccagcgcctg ttttgatgac ccggcatgag attgttttat cgtcagtttg tcgccgccgt
    17401 tatctttggc ccattgctgt tcaaacggcg ggttcagggc ggcaaacagc tcgcgcgaga
    17461 cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagc agcagagaag
    17521 cgaccagcac gagtgagttc tttttcagta agttaacggc cattgcgcac ccttataaat
    17581 ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta acggttttat
    17641 ataccgtttg gtgatttgga agttgaaaag ggaataagac gaaatcctcc ccacaaatgc
    17701 agggaggaaa cagaggtgaa tcagatgccg acgctaaccg tctccggcag tgtgctgccg
    17761 ccatcaatca cattctgtgt acctgttaaa tagctggatt catccgatgc gaggaaggcc
    17821 gccagttcgc cgacttccag cggatcggcg aggcgacgca tcgggattgc tttcgccatt
    17881 tcagtcagca ccgattctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt
    17941 ggcgtgcgaa cgtatccagg gcagatggcg ttaacgcgaa taccagactg cgcgtactcc
    18001 accgccagcg atttagtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg
    18061 cccgggtctg ccaccatatc gccagtgact gaagacatca tcacaatgcg gccatctttg
    18121 cgggcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt tatattgata
    18181 tcaatatgga aatcgcgatc ttcatcgctc atatcgagga agctgcccag acgacaaacg
    18241 cctgcgttat tcaccagaat atcaatgcgt ccttcttttt ccttagcgcg cttaatagct
    18301 gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga
    18361 ccacacagtt cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt
    18421 gcgccatgac gtgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta
    18481 atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc
    18541 gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca
    18601 ctaattcagg aaaatctcaa tttagtcgct gctttatgca gcacctcaag cgttacgtca
    18661 gcgcgcaatc attttcagtg acatcacatg agtaatattc ggacttaatg attcctttgt
    18721 gtctatcaaa tcgggatata tgatcccatt tatctgcaat tatttgatcg caaatattaa
    18781 ggtcaccacc gatgcaactt gaaaagatga ttaccgaagg ctcgaacgcc gcctcggctg
    18841 aaattgaccg cgtatcaacg ctggaaatgt gccggattat caacgatgaa gataaaaccg
    18901 taccactcgc cgttgagcgc gtactgccgg atatcgccgc ggcgatcgat gttatccaca
    18961 cccaggtaag cggcggcggc cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg
    19021 ggattctgga tgccagcgaa tgtccgccga cctacggcgt gaaaccgggc ctggtagttg
    19081 gtttgattgc tggcggcgaa tatgccattc agcacgcggt ggaaggcgca gaagatagcc
    19141 gggaaggtgg tgttaatgat ctgaaaaata tcggcttaac cgcgcaggat gtggtggttg
    19201 gtattgcggc cagcggacgc acgccgtatg tgattgccgg gctggaatac gcgcgccaac
    19261 tcggctgccg tacagtgggc atttcctgta atccggggag tgccgtttca tccaccgcag
    19321 agtttgctat tacgccggta gttggtgccg aagttgttac cggttcttcg cgaatgaaag
    19381 caggcacggc gcagaaactg gtgctcaaca tgctctctac cgggctgatg attaaatccg
    19441 gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc
    19501 gccaggtcaa tatcgtcaaa aacgctaccg gatgtaatgc cgaacaagcg gaagcggcgt
    19561 taattgcttg cgagcgcaac tgtaaaacgg ccatagtgat ggtactgaaa aatctcgatg
    19621 ccgctgaagc taaaaaatgc ctggatcaac acggcggttt tattcgcaag gctctggaaa
    19681 aggaataacc catggctaaa gagatcagca gtgaacttct aaacaccatt cttacccggg
    19741 tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg
    19801 tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg aagggggtca
    19861 ttttgaccag cgatcaggtg caggtggttt ttgggccagg caaagcgcat cgtgccgcaa
    19921 aagccatgag cgagctgctg ggcgaggcac cggtacagga tgccgcagaa atcgccgccc
    19981 agaataaacg ccagttaaaa gccagacaaa cttccggcgt gcaacaattt ctcgccaaat
    20041 tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg ctgctgggga
    20101 tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg
    20161 atgcgctgaa ttttatgaag gtgttcagca aaggcctgtt caccttcctg gtaattctgg
    20221 tgggctataa cgcggcccag gcatttggtg gcacgggcgt aaatggcgca attatcgctg
    20281 cgctcttttt gctcggttat aaccccgctg caaccacggg ttactacgcc ggatttcacg
    20341 atttctttgg tctgcctatc gatccgcgcg gcaatattat cggcgtgttg attgccgcat
    20401 gggcctgcgc ccgcatcgaa tgcatggtgc gccgctttat gccggacgac ctcgacatgc
    20461 tgttgacctc attaatcacc ctgctgatca ccgctacgct tgcgtacctg atcattatgc
    20521 cgctgggcgg ctggctgttt gaaggtatgt catggctgtt tatgcacctg aacagtaatc
    20581 cgctcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc
    20641 agggctttat tcctgtttat ctggcgttaa tggacagcca gggattcaac agcttatttc
    20701 ccatcctttc aatggcaggc gcaggccagg tgggcgcggc gctggcgctc tactggcggg
    20761 cgcaaccgca cagtgcatta cgcagtcagg tccgcggggc gattattccc ggtctgcttg
    20821 gcgttggcga accgctgatt tacggtgtca ccctgccccg catgaaaccg tttgttaccg
    20881 cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct gatagcctgg tggggtctgc
    20941 cgatgggctt aaacagcgcc ttcgggccgt ctggtctggt ggcgctgccg ctgatgactt
    21001 ccgcacaagg catccttccg gcaatggcgg tttacgctgg cggtattctg gtggcatggg
    21061 ttagtgggtt tattttcacc acactctttg gctgccgtaa cgtcaatctg gactgattat
    21121 gaaacggaca atgctctatc tttctctgct ggctattagc tgtagcgtca gcgccgcaaa
    21181 ataccctgtt ttggcagaaa gtagttgatc aagtgtatgc ggcgtcaaag cagtaataaa
    21241 aaggccagtc agttgtgtta ctggctggcc tttaatcaat gaatcagaaa cgcttacagc
    21301 gccatcagct tgtccagcga cggtgcgaag taatagccgc cagtgaccgg tttggtgaaa
    21361 cgcaacatcg catcacgctt accatcggta tcgccaaaca tgctcagcag ttgctgctca
    21421 atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc actggcggtg
    21481 ccgtacggca ggctctgacg aacaatcttc agccctttgc catcttcttt cagatcaacg
    21541 cgggtcaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg
    21601 gtgcgcccaa tcatcatctc ttgatcgtga acgctcatcc gattgagctg cttcaggttg
    21661 tgctcccaac gctgcacaaa cacatagctg ccgcccgcat ccacgccgtc tttgataaca
    21721 gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac aaagccgctc
    21781 aggtcacgct cttcaaccca acggaagcca tggatctctt ctttcacttc aatgcagtca
    21841 ccaaaggctt ccatcgccgc ctgagcgaca gagaagttca cgtcgtgacg cagagaaaga
    21901 atgtggatca acacatcgaa ctgggtcgtc ggtgcaagac ctttaccgta gcccggaaaa
    21961 tctttcagct cttcggcccc aacgccgccg ctcagagcgc gccaggtatt gttaccaaag
    22021 gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg
    22081 gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc
    22141 caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga cattgttcct
    22201 cctgaaaata ataatgccac gcaatccgtg gcattgttgg gcgctatttt acgtgttttt
    22261 tcgcggcggc ggtttgttca gacgcaaatt aacgccgcca gataatttta ctgactttcc
    22321 agtttttcag ggtatcgtcc gaaggcatta acccttcagg accactccat tccccgctga
    22381 actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga ttatcatcac
    22441 tgtcggcctt ttggcaattg ccaaatgctt tgctgtaaag gtcactaaac ggtgtaccga
    22501 ttttaacacc ggtgtcagca ggaatatcgc tatccagcac atcaatgcgg ctgatcgtgc
    22561 cttgatcgcc gttaatcacc atcgccacgt tgttgccttt catcacttca aaaaagcgca
    22621 ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat
    22681 cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc
    22741 tcactttggt ggatgatcca aaccagttcc acgggttagc ggccgaccag ttaactgacg
    22801 acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa cgcagcgatt
    22861 tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc accaaaaagt
    22921 gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga
    22981 ttaacgccac gatatccgcc actaccagcg ccagaccgat accgctaacg gattcaccgt
    23041 tcagccacag ccacggttgc caacaaagta aaaccacttg cgccaacagc aacagaaaat
    23101 agagcacatg ccagatacgg ggaaatgaag cccgccgacc actcagcaaa aatgccagca
    23161 ccgccggaat accgggaatc aaccccagcc agaaattatc gtgatcggga taaaacagat
    23221 ttagcagcgc agtaccctgc tcgcgcgacg ctccggcaat gacaaacagc acccaggttc
    23281 gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcga ccgtgcgcat
    23341 cataatggac aggatgaaac tcagtactct tcatcttcaa tcaaacgctt acccagactc
    23401 agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat gtcgttatct
    23461 tccggcacgt tgatctgaat tttcggacag ccgcgagcaa tcagcttttt ctccagccga
    23521 ttaagcaacg cattagcaat cccacgccca cgaaactcgg gatgcacgcc aagataataa
    23581 gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc accgttcacc
    23641 tcagcgacca gaaacaaact gacgtcatgg ttcatcttac gctcgatgtc catttccgga
    23701 tcgttccacg gactcagcaa gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct
    23761 tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt taaaaggctg
    23821 attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga aaaagtggca
    23881 taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc
    23941 gtaacggatt acgcgatacg atataacatc tggaacttta ttattacaac tcaggccgta
    24001 tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg aaagccggtt
    24061 tggctgccct gacgttgtca ggaatgtcgc aagccatcgc caaagaagaa ccgttaaaaa
    24121 ccagtaacgg acacagcaag ccgaaagcaa aaaaaagcgg cggcagacgt gtcgttgttc
    24181 tcgatccagg ccacggcggg attgataccg gagcgatcgg acgcaacggt tcgaaagaaa
    24241 aacatgtggt gctggcaatt gccaagaatg tccgttccat attgcgtaat catggcattg
    24301 atgcgcgttt aacgcgttct ggcgatacgt ttatcccact gtacgatcgc gttgaaatcg
    24361 cccataaaca tggcgcagat ctgtttatgt caattcatgc cgacggcttt accaacccga
    24421 aagctgccgg tgcttcggta tttgccctct ctaaccgcgg ggcaagtagc gcaatggcaa
    24481 aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaaa gcgactgaca
    24541 aggatcacct attgcagcaa gtgctgtttg atctggtgca aaccgatact attaaaaaca
    24601 gcctgacgct cggctcgcat attctgaaaa agattaagcc ggtgcataaa ctgcacagcc
    24661 gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttccg tcggtgctgg
    24721 tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg gcggcgtttc
    24781 gccagaaaat cgccacagcg attgccgaag gcgtgatcag ttatttccac tggtttgaca
    24841 accagaaagc acactcgaga aagcggtaag tgatgaaacc cgacgcacac ctggttaaac
    24901 agttcttgct caaccttcag gatacaattt gtcaacaact gaccgccgtc gatggcgcag
    24961 agtttgtcga agatagctgg caacgtgaag ctggcggcgg tgggcgtagt cgggtgttgc
    25021 gtaatggtgg tgttttcgaa caagcgggcg tcaacttttc acatgttcac ggtgaggcga
    25081 tgcctgcttc cgccaccgct catcgtccgg aacttgccgg gcgcagtttc gaggcgatgg
    25141 gcgtttcact ggtagtgcat ccgcataatc cgtatgttcc caccagccac gcgaatgtgc
    25201 gcttttttat tgccgaaaaa ccgggtgcag agcccgtctg gtggtttggc ggcggcttcg
    25261 atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc accgcccgtg
    25321 acctgtgcct gccatttggc gaagacgttt atccgcgtta caaaaaatgg tgcgacgaat
    25381 acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc tttgatgatc
    25441 tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc aaaggctaca
    25501 ccgacgctta tttaccaatt gtagagcgac ggaaagcgat ggcctacggc gagcgcgagc
    25561 gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg
    25621 gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg tcaatgccgc
    25681 cactggtacg ctgggagtat gattatcagc caaaagacgg cagcccggaa gcggctttaa
    25741 gtgagtttat taaggtcaga gattgggtgt aactccctca cccccactct cgcatccgct
    25801 gatgcagcgt cagtgacggc ttctcggcaa acagttgctg ataatccgtg gcaaattgcc
    25861 caagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgtgaccacg
    25921 gacttatgag ttcgcggcgc acggcgttca ggcgaatacg tttcagccac gcgttcggac
    25981 caatgcccaa aatggcgtga aatgcgtttt gtagcgtgcg gcggctgaca tgcagttgat
    26041 tacacaagtc cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc
    26101 gggaaagcaa tcggcggtaa ctctgatggc tgatgctttc cgccgtcatc atcggctgcg
    26161 cttcttccag catcgccccc atcgccatga gcaaattatc ccccagcact tttcgcactg
    26221 caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc
    26281 cccacagagc ggctttatgc tgctctttca cttccagcgc cgactggcta cgcagcatat
    26341 gtaatacccg atccggatta tgcaaaaagt tagcctgacg agtgatgaca tcttcagaaa
    26401 gcactacgcc aaggatcgtg taatcgtccg gcgtacttaa ttcaaactcg gttccacccg
    26461 ggcgcgtcgc aatttctgcg cttcccagac attgcgaacc gataaatccc agctcaccgc
    26521 gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac tgacgcagcg
    26581 ccagaccagt gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac
    26641 catgaaactt gcccggatgc agctgatcgt aaatctgctg ccaggcggta attgtcaaag
    26701 catgttcata gacgtccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg
    26761 tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg
    26821 tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat
    26881 ggggcttgag acggtaacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt
    26941 cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcagccact tctcctgccg
    27001 tcattccctg acgtactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga
    27061 taactggcgc gtcgggttcc tttacgggtt gcttaggctg gcggttaaag ccagtaacca
    27121 gccactgggt gtcatcgtct ggccgaccga tctccttgcg gctgatgaca cgcccggtac
    27181 gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca
    27241 ccaccagcgt taggcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg
    27301 ctttgagcat ggcatcagct gcatctatcg cggcgaccat gccgtccact tccagcaatc
    27361 ccagtgcatt gatcatcggc aacctccgtt acgcacgctg gattggatta cgcgcgattt
    27421 ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg ctaccggtta
    27481 aaaatgctgc cgagtagttg gtttcagacg gtggcgggac ataggtcacc agctgcacgt
    27541 cggcagattt caacgctgca tcaatgccgt aggtcgcttc cagcggcggt gccaccagat
    27601 acgccatcgg atcgccaagc gtgatcccgg cggttgatga gagataagaa ccggtacgcg
    27661 aaactacatg tgccaggaag gctgtgtttt ctgcgtcatt agcccactgg aaagcagcgc
    27721 cattttcaat attcgcaacc atcgcatcca gaccagcacg cacttccgcc gggtttggtc
    27781 caccaagcat aatcagcacc tcaccggcag tcggtgacgg gccgtgtgca gcgcccgcat
    27841 acagcgagcg accatatacc acttcgacca tcgcctgctt ggtcgcttcg tcagccgcaa
    27901 tgtaggtgac gtcatctgaa tcagcagaaa tcagcccgag gctacgaata tgcggcggca
    27961 atttcagttc acgtgcaaat tcggcgttaa cggaggcaat cacccgcatg gcggttaccg
    28021 acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgccgg
    28081 atgctttctg ctccagcata cgtttcgcca aatccacaat gacggctgcg gcttcaaccg
    28141 gcggcgtgcc gccctggtga atgttagaga tacaagtgcg gtcagcctca acggtggtcg
    28201 ccatacgcgg cgagtaaacg gcgtagcagg agaggttttc tgactgcccc aaccccggac
    28261 gttcgcccac cagcaggatc actactttcg caccgaggat ctcaccgatc tgatcttcaa
    28321 tcttcacgcg accataacgt acaaagaatg gcgtaccgac tttcagcccg gcttgcttca
    28381 ggcccgccat cagcggcggc aggatctctt cgtagttcac ggtgatcgca tcagttgaca
    28441 ggccatcaga aataacgacc tgcacgtccg gattagcaac acactgtgct ttcagcgctt
    28501 caacagcttc tgcacacagg cggcggccca tatccgggcg agtcaggtag agatttttgt
    28561 cgctgatttc ggagcggact tccagcaagc cctgcgcttt cacccactct tccggtactt
    28621 ctttcagaac ggtatctttc gaacgagagt gatcggccag gaaacgcagc aacgcctggg
    28681 tgcgcggacg cggaccggca cgaccggtac aaacgcgggc cacggtgctg cgacgcagtt
    28741 ctgttaatac gtctgcgtga tgcgggtttt caacaccaat ccacgctttt gcttccgcag
    28801 aacctaaatc cagcgcgcag ctttccgagg tcaccggtgc cgcacaggtg gtggtggcgc
    28861 actttgcttc tgacggcgcg ggggccgctt gtcccattga cgccatcacg ctgcgtacaa
    28921 tttcttcaat ctgtttttga tccatgatgt gttatctccg cgtcatcaga agaacagtga
    28981 cggatcgccc gcccgtttgg tcaggcgacc gtttgccata atgcccatgc tttccagcca
    29041 gcgttcaaac tccggtgacg ggcgcagatt cagtaactga cgcacagtgg cagtgtcgtg
    29101 gaatgcggtg gtctgatagt tgagcatgat gtcatcgccc agcggcatcc ccatgatgta
    29161 gttgcagcct gcggtggcga gcaggatcat caggttttcg ttgaggttct ggtcagcgtc
    29221 agcgtggttg gtgtagcagc agtcacagcc catagagatg ccgctcagtt tgcccataaa
    29281 gtgatcttct aagcctgcgc ggataatctg gcggtcgttg tagagatact ccggcccaat
    29341 aaagccgacc acagtgttga cgataaacgg atcgtaatga cgcgccagcc catagttacg
    29401 cgcttccatc gtcacctggt ctgcgccgaa gttcgcgcca gcagacagcg ccgaaccttg
    29461 tccggtttcg aagtagaggc agttttcccc ggcgatacgg ttgaactccg cgcccactgc
    29521 gcgtgcttca tcgagcatcg ccagctctac gccaaactct ttcagccctt tttcgctgcc
    29581 gcagatactc tggaaaatca gcccgcctgg tgcgccgcga cgaatcgctt cgatttgggt
    29641 agtgacgtgc gccagtacgc agccctgagt tgggatgttg aatttgtcga tcacgccata
    29701 gatggtatcc aacacgcggc ttaagttttc cacgtcgtca gtcaccgggt taacgccgat
    29761 caccgcatcg cccaccccga aggaaagccc ttcgtagatt tgcgcggcga tactttgcac
    29821 gtcgtcacgg gtatcgttcg gctgcaagcg ggcgctgaag gtgcccggaa taccgatggt
    29881 ggtattggcc tttttgatca ccggcatttt cttcgcgccg tagatcaggt ccgcgttgga
    29941 gcaaatcttc gctaccgccg ccaccacttc cgaggtcagc cctttgcggg taaaggcaat
    30001 gtcatccacg ctggtttcat cgctcagcac atactcacgc agttcgctga tgctccagtt
    30061 tttaatctgg ttgtaggccg tttcgttaac gtcgtcctga atcagccgcg tcacgcagtc
    30121 atcttcatag gcaatcaccg gattattgcg gatgtccgct acagtcattt ccgacaacac
    30181 ctgctttgcc gccacgcgct cctgtgagct tgccgcagca acgcccgcca gcacatcccc
    30241 cgaacgcagt tcgttggctt tagccagcac ctcttttaca tccttaaact gatatacatt
    30301 gccgaacaat gtggtcttta gtttcataag tcgttccctc aggaaggaaa tgcgagtgat
    30361 ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg taccaatgtc gatatagtcc
    30421 cccgcgcgga caatcacttc gtcaatgact gccaacggga gttgttgtag ctgtgggcgc
    30481 aacaacatgc ccagagcttt accaaagtcc tgcccggcca ccaccagcag gggatgcgga
    30541 ttcggaaaac gcgcgacgaa atcgaccagc gcgttgatga ccgtcagtac cgcggcgtaa
    30601 cgcacaggca gcgaggcggg aagcgccagc acgtacgcgt cagttttggg gcaaagatcc
    30661 agttgaatca gcgcctgttg ccaggcactg accagatccg tttcatcaat cgggatcgcc
    30721 accggcaaat tgcgcagcgg cagttgtacg ccctccagcc atattgtgct gccagagagc
    30781 gaaagggtat gtgcacccgc gccaatcacc gtggcgcgca cggtttgcgc cggaaactgc
    30841 acgttcatct cacgcagacg cggatggtca tgcagcgccg ttgccagaag cgggccgata
    30901 tcggcaaaac agaacgggtc ggcgggctgg tggcgataac attcgcctac cccgccagaa
    30961 agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat caatgcctgc
    31021 gcgagcggcg agagcgttcc gtcaatcact tcgacaatca gttccgccat ccggcgggta
    31081 acctgcgcca attgcgcgcc ggtcagcgaa cgggcgtcag tgcctgggcc gaagcactca
    31141 tccacaatca tctgccccgg tttatgagcg taaaccacgc gcccctggct gtcggtttcc
    31201 agcaggcgac cgccgacgtt gaggcaggca gtgccgctga tttttccggc atcgaacagg
    31261 gcgtagttcg cggtgccacc gccgatgtcg atattcagta cccgacacag ccgttgttca
    31321 gaaagggttt gcgccccggc accgtgaccg gcgatcacgg attcgagatg cggcccggcg
    31381 ctggcgacga caaaatcgcc cagcgaccga gagagcgcca tcaccgccgg gcgagcgttg
    31441 cgggttttcg cgctttcacc ggtgatgatg atggcaccag aatcaacgct ttccggcgca
    31501 atgcccgcag cctgatattg ctcgaggatt aaggttttca gttccgcttc ttttaaacca
    31561 ccctgtttat cgacaggggt aaagaacacc gggctttgcc agctaatttc gcgtttgatg
    31621 aattcgtagc gcggcacctg cgacaccgcc gcacggttaa ccagctccag gtgggagaag
    31681 atcacctggg tggtggtggt gccgatatcg ataccgacgc tcagtagctg gcgagtgttc
    31741 acgattgcgc ctccgcttcg gttttagccg cgctcgcgtc ttcttttggc accagcatca
    31801 tcgccacgcc aattgccgtt acgccgccga tcaatttgcc gacaatcatc gggaagatca
    31861 tggcgttcat gttggcagcg gcgaagccta aatggtcgcc cagcgcgaaa gcagcggaaa
    31921 cggcgaaggc acagttgatg actttgccgc gggtatccat ctgcttcatc atgccaaaca
    31981 tcggaatgtt gttggcaagc gttgccacca tgccggctgc cgcgatattg ttcatattca
    32041 gcaccttacc gacgctcatc agcggttttt caaaccagcg agtcagcagc agtaccattg
    32101 gatacgcccc taacagaacg caggagatcg agccgataac ttcaatggcg cgcatcactt
    32161 caccgggttt atcgccaggg gccataaaga taggatcaag gcccgggatc agttcccagc
    32221 caaggaggaa tttcactacc gcagcggcaa gaccgagggt gatcaatgca acgaggaatt
    32281 tggcgaagat ctggaagccg ttgatcattt tttccgggat gaatttcagc cccagcgcca
    32341 ccagcaccgc aacgataagt accgggatca tgttcatcag gatcagcgca aaggtgaatt
    32401 caacaggctg gccgttgatc tgcacaccgg agtacatcgc taccagacca ccagcgatac
    32461 agccaatcgg aatggtcact atgcccgcca gcacgccgag cgccagataa cgacggtcag
    32521 aaggttcgat aatgccgagc gccaccggaa tggaaaacac aatcgttggc cccatcatcg
    32581 acccgagaat taacccagag tatagccacg ctgctacgtc accgcccgcc agctctttgg
    32641 cgaggaagaa gccgcccata tcgcacgcca gcagtgttcc ggcgaacatt gacgggtttg
    32701 cgccgagcat ttcgtaaacc gggataatca ccggtccgag aacgtgggcc agtaccggtg
    32761 ccagtgcggt cataccgacc atagccaggc ccagagcgcc cattgccata aagccttctt
    32821 cgaactgacc gccggatcct tcgatacttt taccgaactt accgaggaaa cgagcagaac
    32881 cgccgaactg cgacaggatc ctgtctacgg cagctatcag cataaagaac atcatgatgt
    32941 acatgatgat ttcgttaatt cccatcgcct ttactccctg ttagttgtta tttattggcg
    33001 gatgcggcgt gaacgcctta tccgccctac atgtgcaatc ccgaaggcca gataagacgc
    33061 ggcaagcgtc gcatccggca attgcaccgc gccactggcg gatgcggcgt gaacgcctta
    33121 tccgccctac atgtgcaatc ccgtaggccg gataagacgc ggcaagcgtc gcatccggca
    33181 tttattgcgc cgctgcgtac aggccgacaa tctgctcgag gctggcggta cgcgggttac
    33241 tgcgcagaca aatatcttcc tgcgcggcct gcgcccatgc accgtaatgc ccggttgttg
    33301 cacccacatc agccagccgc ttgttgatcc caacttccgc tatcagctca cttaccgcat
    33361 taatagcgtc acgatcgtcg gattttttag ttcgcagcgc ccgaccaatc tgactaaacc
    33421 gatcacgaca aaccatccgg ttaaattcca tcaccgttgg cagcaacatg gcgttcgcca
    33481 gaccgtgcgg aatatgcagc gccgcccctg gctgatgcgc catcgcgtgg cataacccaa
    33541 gcccggcgct ggaaaacgcc attcccgcca tacatgaagc cagcaacatg ctctcgcggg
    33601 cgacaaggtc gtgaccgtag cccaccgctt tcggcagcga ttgaccaatc atcgcaatgg
    33661 cgccaatcgc caggctgtcg gtaaacggtg tagcgttcag cgcgctgtac gcttcaatgg
    33721 catgggttaa cgcatcaatg ccagtcatcg ccgtgacatg cgacggcacg ccttcggtta
    33781 gcacagcgtc gagaatcgcg acatccggca tcagcgaggc atgggctaac acctgtttgc
    33841 gcccgcttac cgcatcgata atcaccgtca cgttggtggt ttcagagccg gttccggcgg
    33901 tcgttggaat ggcaatcaac ggcaggcgcg gttgcagaac gctggtttct ggcatttctg
    33961 ccagcgtgct atccgggttc gtcaccagca acgccacggc tttcgccgca tccagcaccg
    34021 agccgccgcc aaacgcgatc accccgtcac aacctgattc acgcaactgc gccacggctg
    34081 cacacacgtc ggtgatgcac ggttcgccca ccggacatgg ccagagcgtc atggcgatac
    34141 ctttaacggc caggctacgc gtcagcccag cggtcatccc tgcctgatgc aaaaagctgt
    34201 ctgccatcac gaacagatgt ttcaacccac gcgtttgcgc ttgctgccca caactgctca
    34261 ccgcgcccgg accgcaaagc gtcaccggtg gaacgctaaa tgtttttacc cgttgcagat
    34321 tcagggtatc gaacgcctga aagagcgcgg tctgcaattc attttgcata tagcccctcc
    34381 gctttctctc gcccactact ggcgattgcc agcggggtca taaacaaact gtgctgcggt
    34441 aaatgcacct gtaacgccgg aaattgtttg caaaacagcg ccgctacgcc cggttgcata
    34501 caggaaccgc ccgccagcca taaatccgta atgccctgcc cttcaatatg acgggccaca
    34561 atgtccgcca ttttttcgta caccggtttc accgcgggcc aaatctcgtc accgtgaccg
    34621 cgtttgtact gctctgcctc ttccagcgaa atacggcgat ttccggcgag agtcagagag
    34681 atgtgatgtc cgccggtggc ttcatccgcc gagtacgtca ctttgccctt tttcacgatg
    34741 gcaatgccgg tagtgccgcc gccgatatcc actacaccgg cgttgtccag ttgcagcaga
    34801 tccgcaactg cggttggctc atccagcaca tggctcactt ccagtccggc agactccagc
    34861 acgttgatgg aaatacgcgg gtccgtacca ggaggaaatg aggtcgccgc atggctaaaa
    34921 cggcgaccga attgctgctc aagggtgtcg agatggcgac gaacaatggt gaccgcaccg
    34981 aagaaatccc agacgatgcc gtcgcggacc acatcggccc agtcgaggca taccgccacc
    35041 ggctgaccgt cgcggtcgac aaccatcgac accacatcgc aggtgcccag atccaccccc
    35101 agccacagcg gagattccgt cgcggcgggc gtctggttac acagcgttgc cgccgtttgc
    35161 agacgtgggg tgagccattg ttcatcgtgc gccatcggtt actccttaaa caatgcgaaa
    35221 cgcatcgact aatacacagc gacgcagacg gacaaacgtc cgcgcgctgg ttaccccttc
    35281 accggttggc gtggtgatgg tcatcgtggt ccagccttcc ccgcccagcc ccagcccggc
    35341 aatacacggt ccgttcttaa cgaaaatgct ggtatcaatg gcgttcgcca tctggttcat
    35401 gttttcgata ttgcgcgagt gcattgccgc cgtgtggtgg caaccgcctt ccagtttcac
    35461 cgccagcgca atggcatccg ccacgttggc gacgcgcacg acgggcagca ccggcatcat
    35521 cagttcggtc acggcaaacg gatgttctgc ggtggtttct acaaacagca gtcgcgtttg
    35581 ttccggaact ttaaggccga ttgccgccgc gattttcgcc gcatcgcgac caacccagtc
    35641 acggctgacg gtgccttttc cgcgctcgtc gatatttttc agcaacaccg attgcagttg
    35701 ctgcgcctgc tctgcggtca gtttcaccgc atgctggcct tccatcagac gcatcagttc
    35761 atcggctacg ctatcaacaa caatcagtac cttttcgtcg gcacaaatga tgttgttatc
    35821 gaaagaagcg cctttgacga tggactgagc agcacgggcg aggtcggcgg tttcatccac
    35881 cactaccggc gggttgccag cgcctgcggc aatcagacgt ttattggtgt gtttacgcgc
    35941 cgcttctact accgcttcgc cgccggtcac caccaaaagg ccgatgcccg gaaacttgaa
    36001 caagcgttgc gcggtttcga tatccggatt tgttacggta accagtaagt tttccggccc
    36061 gcctgcggca acaatcgcca ggttgagcag cgtaatcgcc cgctgggaga cttttttcgc
    36121 cgccggatgc ggggcaaaaa tgacgctgtt gcccgcggca atcaggctga tggcgttgtt
    36181 aattacggtt gccgccgggt tagtggaagg cgtcactgaa gccaccacgc cccagggtgc
    36241 gttttcaatc agggtcaggc cgttgtcgcc agtcagcact tgcggagaga ggcactcaac
    36301 gcctggtgtg ccgcgcgcct gagcgacgtt tttggcaaat ttatcttcaa cgcgccccat
    36361 gccggtttca ctgacggcaa gttccgctaa atctctggcg tgtttttcgc ctgcttcacg
    36421 aatggcagca atggctaact ggcgcattgc cacgcttttt aacccttgct gggcgacttt
    36481 ggctgccgca acggcgtcat ccagggacgc gaaaacgccc atctcatgaa cggcggcggg
    36541 cggcgtgtca ctgctttgca ttttcagcag taccgctttc accacctgtt caatatcctg
    36601 ttgattcatg atgttctgcc ttatttgtgg aaaatgacct gaccgccaga caccacctca
    36661 tcgacaatgc caatcacgca cagatcgacc ggtgacgttt cgcttttatg cgcctggcgg
    36721 gcggaactgc cactcaccag taacacccac tccccggttc ccgcgccaat attgtcgatg
    36781 gcgacggcgc attgcccgtc aggattacct tgtggatcaa tcatttccac catcagcaat
    36841 ttgtcatgcg ccagtccgtg atggcgtacg gtacaaacaa tttgtccagt gacgactgcc
    36901 agtttcatac ccgcctccgt ggcgtatttc aggtaaaagc tccccctacc ctccgcagaa
    36961 ggtaaaatga aaaaggagag agcgtgacgt ccgaatcgac gtcacacagg gtgattacag
    37021 gttgctgcta tcgcctttca ggccgatcgg gaagacttct tccaggtcgc cgtgcggacg
    37081 cgggataacg tgtacagata ccagctcgcc gatacgctgt gccgctgcgg cacctgcatc
    37141 ggtcgcggct ttacaggctg caacatcacc acgaaccatg gcagtacaca ggccgccgcc
    37201 aatctgctta acaccaacca gcttgacgcg cgcagcttta accatcgcgt cagaagcctc
    37261 aatcagtgca accaggcccc gggtttcgat cattcctaat gcttccattg tgctttcctc
    37321 tttatcaggg tccagaacgg gaccgttcat tcaaccagtg tttgtaaact gctttcgcgg
    37381 ttcacttctg tctgacgcgg cacagctgcc accagcgcca gttcgataat ttcctgcacg
    37441 ctacaaccac gagagagatc gtgcatcggc gcggcaagtc cttgtatcaa tggcccgacg
    37501 gcacgatatc cgccaagtcg ttgtgcgatt ttgtagccaa tatttccggc ttccagcgac
    37561 ggaaaaacca tcacattagc tttgccctgt aacgggctgg caggcgcttt ttgcgccgcc
    37621 acttccggca cgaaggcggc gtcaaactgc aactcgccat cgacaaccag ctttggtgcg
    37681 cgctcacgga cgatttctgt cgcctgctgg acgttggcaa cacaggggtg acgggcacta
    37741 ccgttgctgg aaaacgacag catcgccacg cgcggttctt ctccggtgat ggcgcgccag
    37801 gtttcggcgc tggcaagcgc gatatccgcc agttgtgccg ccgtcggctg tggcaccacg
    37861 ctgcaatcgg caaaacccaa cgccggaccg ctgtactgtg gcagcatcag gaaaatggat
    37921 gagagcgttt tacagcctgg ctgcaagccg ataatgcgta atccggcacg cagcacattc
    37981 gccgtggaag agaggttgcc cgcgatacag acatccgctt taccggcgct gaccattgca
    38041 gcggcgaaca tcagcggatc ggtaagtttt tccagcgcat ccggcggcgt tttttcgccc
    38101 gcgcgggcca gccagcgatg agcaaattct tcccgcattg cgaggttgcc atgcggatct
    38161 atcacctgta gcccgtccat cgccacgccg tgactgagcg caaactgacg aagttcaaac
    38221 ggattggcga ccagaatggg cgttgccaga ccttgttgat gtaaatattg cgcagctttc
    38281 agcacacgtt gatctaacgc atccggaaaa actactctgg cgggcgctcg caacgccagt
    38341 tcacgacaac gtttaataat cattgcgttt cccccagtcg ttgttggatc tgcttcaccg
    38401 ttagcggctg cttcgttacg ctcaggatca tcatcacgta gaccgtgctg gaaaggcggt
    38461 tcagcgcctg caaaatgtcc gggcgtaata cttcaaagct gcgggtgata aagacctgcg
    38521 ccgctacggt ttctgtttcc cgcactttgg tgcgtagcag attgagcaac gccgcatcgc
    38581 gcccgtggct ggcttccggc accagatgat cgtggtcgag ataacgcagc ggctggtgag
    38641 aaagccgatg cagatcttcg tcagaaagcc cgacaatcgc ttgctcgccc agcggttccc
    38701 ccagcgcatc ggcacgcata atgttgccaa gacgcgagcg gatgtccgcc agccacggct
    38761 gccacggttc tgccagttca atttgcagcc acactgccag cgcaatggtg ctgtcgagaa
    38821 cagcgcgaaa acccagacgc ggatcgcttt tggcaaccat tttttccgcc gacaggtgag
    38881 tcagcgtatc cggctttttt gccaccggct ggcggcacag ttcgcagcac gcctgcggat
    38941 gttcatcgct actggtcagc ccgtgaacgg gctgcggctg ctgctgttca tcgtcaacaa
    39001 acaggcggcc ctgctcgtca ataaacttga tgcgcagatg gcggctttcc agtaactccc
    39061 gggcagaggg cgtcaggcga ctgtccgcag ggagatggat ctctgcgcct tcgctgagcg
    39121 tatggttcgc tcttagccat gcttcggtga tgaaatcttt cataaggatt gccagttagc
    39181 cggccaggcg acatacagga atttcacgct ggatgtcgta ccaaattcga tgctggagcc
    39241 tttcgggata aacatcacat cgcccgcttt ggcgatcata gtttcgcctt cgtggcgaac
    39301 atgcagctcc ccttccagca ccatgtcgat ttcgtcgtag ttcagggtcc acggaaagaa
    39361 ggcgttctcc cactgcatga aaccagcggc catactgctg ccgtcgtctc cggtcacgag
    39421 atcagtcaag cctacgcagt gcggttccgc gccatcgaaa cgaccaaact tgacgctgct
    39481 gccatcaata actttgatgc cgcctttgcc ggtcaccgat ttaaagctcg gctgcagtgc
    39541 accctgttcc agcgactgtt tttccttcat cactttttcc atcagctgcg cgaccaggct
    39601 ttcggtaaac tgaccttccg gcagttgggc gataatggtt tcacggatgc gctggctttc
    39661 ggttttatcg gctgatgcgc tggcgggaac agacgccgtt accgggatcg attcatcaca
    39721 ttcggtgatg gtaaaaccca acagatcagc cacttcacga gcttccgggg taataatgct
    39781 ggcgcgcaga accacggaca ttgccagttc gccgcgcgcg tgtgcttcac gaatatcatt
    39841 cgctgtgatg agttttttca cctgcttcct cctgtttggt gagagatgcc agataatcca
    39901 ccagttgctg cacgctttgc gggtcgtggc tgtttagctc gaaaatcggc tcttcgaagc
    39961 ccgtctcaag caataacttt cgcgttgcgg cgacgtcagc atctggcatg tccgttttac
    40021 tgatgacggc gatttgccgt ttgctgacgc caatatccag caacccggca ggcaaccgac
    40081 tttccgggtc attcgcgccg tggacataga tcagcatatc cacatcctgt agcgtggtaa
    40141 ttaaggcgtg ataccagcgg ggatggctaa aatattcacc cggcgtatca atatcgccat
    40201 tatcattaaa ttccacggcc tgtgtttttc ttgcgagggt ataattcccc tgcaacgcat
    40261 taaataacgt tgtttttcct gccccgacgg tacccacaaa cgcaattcgt ttcatgaatg
    40321 cctcgtatta acttttggtc atttcacaaa gcgtataatt taataagcgt cctaaaccac
    40381 tgacggtttg tgataacgct tcttctactg cgcccaccga gccataaatc accagcgcgc
    40441 cactaaaacg atcgagaaaa ccgatatgca catcggcggc tttcaacgct aaatcaccgg
    40501 cgatcatcgc cgtttctccg ggagttagcg tcataatgcc gattgcgccc gcatccggaa
    40561 cgccgatttt cttcgccagt tcctcgccgg gatgcgcgat gagatgcgcc agtgtgacct
    40621 gtttaccggg cacaaattcc tgaatgatgc gttctttatc catgagtcgc cgcctgtgaa
    40681 aaactttcgt gtatcgtgac aatttttcgt cagagtaaat aacaaaattc ggcaagcgcg
    40741 tttaaaaggt gaggtagatc actaagaatg gagagatatt tgctgtggtg cataaatt
//
LOCUS       DAJOTB010000024        40076 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJOTB010000024 DAJOTB010000000
VERSION     DAJOTB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.024"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            187..1620
                     /locus_tag="OHN28_001308"
     CDS             187..1620
                     /locus_tag="OHN28_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCQ2296742.1"
                     /translation="MDAENKIKNADSIAKQKIDEAQIKAAKTVNEANYQAQMTISNAN
                     SEAIAITKDARDARLKAKERLDNANNKANELISNANDNAVKIISDAEERAKEIAGSAY
                     EAKEFAEKYEAVAKSMKNKIEGYGDEWIIPNRSVLDELAENYEFTDAGKELQKARELT
                     KSLIKTNKAASCDYVEQNRRNTAINFVLDAFNGRVDSILSKVKHNNFGKLSQEIKDAF
                     QLVNYNGSAFRSAKISDIYLQARLNELKWGVAVNEIMLEEKEEQRRIKEQLREEERAR
                     REYEKAIKEAEKEEKAIQQAINKATKELMLANEEQRLALEQKIAELQLKYEEAEAKNQ
                     RAISMAQQTRSGHVYIISNIGSFGEDVYKIGMTRRLEPLDRVRELGDASVPFSFDVHA
                     MIYSDDAPSLENHLHKVFNEKQVNKINSRKEFFNVNLKEIKSVIEDMNINAHWTMFAE
                     AKEYRESLAIEQERKAATSANDELHVA"
     gene            1703..2755
                     /locus_tag="OHN28_001309"
     CDS             1703..2755
                     /locus_tag="OHN28_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2296743.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(2860..3396)
                     /gene="rcdA"
                     /locus_tag="OHN28_001310"
     CDS             complement(2860..3396)
                     /gene="rcdA"
                     /locus_tag="OHN28_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCQ2296744.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            3480..4688
                     /locus_tag="OHN28_001311"
     CDS             3480..4688
                     /locus_tag="OHN28_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296745.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMVLSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATMHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4688..5503
                     /gene="ybjI"
                     /locus_tag="OHN28_001312"
     CDS             4688..5503
                     /gene="ybjI"
                     /locus_tag="OHN28_001312"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCQ2296746.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            5589..5873
                     /locus_tag="OHN28_001313"
     CDS             5589..5873
                     /locus_tag="OHN28_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296747.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(5914..7146)
                     /gene="mdfA"
                     /locus_tag="OHN28_001314"
     CDS             complement(5914..7146)
                     /gene="mdfA"
                     /locus_tag="OHN28_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCQ2296748.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            7431..8027
                     /gene="ybjG"
                     /locus_tag="OHN28_001315"
     CDS             7431..8027
                     /gene="ybjG"
                     /locus_tag="OHN28_001315"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2296749.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            8085..8843
                     /gene="deoR"
                     /locus_tag="OHN28_001316"
     CDS             8085..8843
                     /gene="deoR"
                     /locus_tag="OHN28_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCQ2296750.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHISKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(8890..10092)
                     /gene="dacC"
                     /locus_tag="OHN28_001317"
     CDS             complement(8890..10092)
                     /gene="dacC"
                     /locus_tag="OHN28_001317"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2296751.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            10339..10965
                     /gene="gstB"
                     /locus_tag="OHN28_001318"
     CDS             10339..10965
                     /gene="gstB"
                     /locus_tag="OHN28_001318"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCQ2296752.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDTDF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10962..12077)
                     /gene="yliI"
                     /locus_tag="OHN28_001319"
     CDS             complement(10962..12077)
                     /gene="yliI"
                     /locus_tag="OHN28_001319"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCQ2296753.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(12188..12571)
                     /gene="bssR"
                     /locus_tag="OHN28_001320"
     CDS             complement(12188..12571)
                     /gene="bssR"
                     /locus_tag="OHN28_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCQ2296754.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            12784..14109
                     /gene="rimO"
                     /locus_tag="OHN28_001321"
     CDS             12784..14109
                     /gene="rimO"
                     /locus_tag="OHN28_001321"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCQ2296755.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(14156..15484)
                     /locus_tag="OHN28_001322"
     CDS             complement(14156..15484)
                     /locus_tag="OHN28_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2296756.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15492..17839)
                     /locus_tag="OHN28_001323"
                     /pseudo
     CDS             complement(15492..17839)
                     /locus_tag="OHN28_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(18016..18927)
                     /gene="gsiD"
                     /locus_tag="OHN28_001324"
     CDS             complement(18016..18927)
                     /gene="gsiD"
                     /locus_tag="OHN28_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCQ2296757.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(18930..19850)
                     /gene="gsiC"
                     /locus_tag="OHN28_001325"
     CDS             complement(18930..19850)
                     /gene="gsiC"
                     /locus_tag="OHN28_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCQ2296758.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGILLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(19868..21406)
                     /gene="gsiB"
                     /locus_tag="OHN28_001326"
     CDS             complement(19868..21406)
                     /gene="gsiB"
                     /locus_tag="OHN28_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCQ2296759.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(21426..23297)
                     /gene="gsiA"
                     /locus_tag="OHN28_001327"
     CDS             complement(21426..23297)
                     /gene="gsiA"
                     /locus_tag="OHN28_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCQ2296760.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(23284..24249)
                     /gene="iaaA"
                     /locus_tag="OHN28_001328"
     CDS             complement(23284..24249)
                     /gene="iaaA"
                     /locus_tag="OHN28_001328"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2296761.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACLMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            24453..25688
                     /gene="moeA"
                     /locus_tag="OHN28_001329"
     CDS             24453..25688
                     /gene="moeA"
                     /locus_tag="OHN28_001329"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCQ2296762.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            25688..26437
                     /gene="moeB"
                     /locus_tag="OHN28_001330"
     CDS             25688..26437
                     /gene="moeB"
                     /locus_tag="OHN28_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCQ2296763.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(26513..27175)
                     /gene="fsa"
                     /locus_tag="OHN28_001331"
     CDS             complement(26513..27175)
                     /gene="fsa"
                     /locus_tag="OHN28_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2296764.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAVGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            27306..28205
                     /locus_tag="OHN28_001332"
     CDS             27306..28205
                     /locus_tag="OHN28_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCQ2296765.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            28211..30643
                     /locus_tag="OHN28_001333"
     CDS             28211..30643
                     /locus_tag="OHN28_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCQ2296766.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            30789..31604
                     /gene="ybiV"
                     /locus_tag="OHN28_001334"
     CDS             30789..31604
                     /gene="ybiV"
                     /locus_tag="OHN28_001334"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCQ2296767.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHITLDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(31804..33396)
                     /locus_tag="OHN28_001335"
     CDS             complement(31804..33396)
                     /locus_tag="OHN28_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCQ2296768.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            33615..34535
                     /gene="ldtB"
                     /locus_tag="OHN28_001336"
     CDS             33615..34535
                     /gene="ldtB"
                     /locus_tag="OHN28_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2296769.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(34593..35711)
                     /locus_tag="OHN28_001337"
     CDS             complement(34593..35711)
                     /locus_tag="OHN28_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCQ2296770.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(35708..36175)
                     /gene="mntR"
                     /locus_tag="OHN28_001338"
     CDS             complement(35708..36175)
                     /gene="mntR"
                     /locus_tag="OHN28_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCQ2296771.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            36361..36489
                     /gene="mntS"
                     /locus_tag="OHN28_001339"
     CDS             36361..36489
                     /gene="mntS"
                     /locus_tag="OHN28_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCQ2296772.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            36761..38344
                     /gene="opgE"
                     /locus_tag="OHN28_001340"
     CDS             36761..38344
                     /gene="opgE"
                     /locus_tag="OHN28_001340"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2296773.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(38393..38908)
                     /gene="ompX"
                     /locus_tag="OHN28_001341"
     CDS             complement(38393..38908)
                     /gene="ompX"
                     /locus_tag="OHN28_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCQ2296774.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(38961..39026)
                     /locus_tag="OHN28_001342"
     CDS             complement(38961..39026)
                     /locus_tag="OHN28_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296775.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            39261..>40076
                     /gene="rhtA"
                     /locus_tag="OHN28_001343"
     CDS             39261..>40076
                     /gene="rhtA"
                     /locus_tag="OHN28_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCQ2296776.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAI"
BASE COUNT        10295 a        10406 c         9579 g         9796 t
ORIGIN      
        1 gtaatattat ttaaacaaag taaaaaacac aaagcggcta tagataacct gacagctaac
       61 aacatagccc tttctaacca actgattgaa aatcaagaaa aactagcaca gaccgtacga
      121 gaattatcag agcttgaagg gcgagcagca ccattgtggc aatacgaaga attgcacagc
      181 gcagtaatgg atgcagagaa taagataaaa aatgcggact caatagccaa gcaaaaaata
      241 gatgaagccc aaattaaggc ggctaaaaca gtaaacgaag caaattatca agctcagatg
      301 acaataagca acgctaatag cgaagctata gcaatcacca aagacgctcg cgatgcacgc
      361 ctgaaagcca aagaacgtct tgataatgct aataataaag caaatgagct aatctcaaat
      421 gctaatgaca atgcagtaaa aattatttct gatgcagaag aaagagcaaa ggagattgct
      481 ggttcagcat atgaagctaa agagtttgca gaaaaatatg aagcagttgc aaaatcaatg
      541 aaaaataaaa ttgaaggtta tggtgatgaa tggatcatcc ctaaccgtag tgtacttgat
      601 gagctggcag aaaattatga atttacagat gcaggcaagg aattacaaaa agccagagag
      661 ttaaccaaat cattaataaa aactaataaa gctgcttcgt gtgattatgt tgagcaaaac
      721 aggcgtaata ctgccataaa ctttgttttg gatgccttca atggaagagt tgacagtatt
      781 ttatcaaaag tcaaacacaa caattttgga aaactttccc aagaaataaa agatgcattt
      841 caactagtaa attacaatgg ctctgccttt agatccgcaa aaataagtga catctatctt
      901 caggcaagac tcaacgagct aaaatggggt gtagcagtta atgaaattat gctcgaagaa
      961 aaagaagagc aaagaaggat taaagaacag cttcgtgaag aggaaagagc tcgtagggaa
     1021 tatgaaaaag cgataaaaga agctgaaaaa gaagaaaaag ctattcagca agctataaat
     1081 aaagcaacga aagaacttat gcttgcaaat gaagaacaac gcttagcttt agagcaaaaa
     1141 atagctgaac tacagttaaa atatgaagaa gctgaagcta aaaatcaacg agctatttct
     1201 atggctcaac aaaccagatc aggccacgtc tatatcatta gtaatattgg ttcatttggc
     1261 gaagatgtat ataaaattgg aatgacacgc cgccttgaac cacttgatcg tgttcgtgaa
     1321 ctcggagatg ctagtgttcc tttttcgttc gatgttcatg cgatgattta tagtgatgat
     1381 gcaccatcat tagaaaacca tctgcataaa gttttcaacg aaaagcaggt caataaaatt
     1441 aactcacgaa aagagttttt taacgtaaat ttaaaagaaa tcaaatcagt tattgaagac
     1501 atgaacatca acgcccactg gacaatgttt gcagaggcga aagaatatag agagtctcta
     1561 gctattgagc aggagcgcaa agcagccact tccgccaacg atgaactaca tgttgcttag
     1621 caatgtatgt ttcatagtaa tcacacattg attactggtt acatatacag ttaaatttag
     1681 ccctctgata tgagggcttt ttatggcagt acgaaaacta accacaggga aatggctttg
     1741 cgaatgttac cccgccggac gaagtgggcg tcgtgtgcgt aaacaattcg ctaccaaagg
     1801 cgaagcactg gcttttgagc gccacacgat ggaagaaacc gaagcaaagc cctggctggg
     1861 tgaatcagtg gatcgtcgaa cactgaaaga cgtggttgag ctatggttca aactacatgg
     1921 taaatctctg acagctgggc agcatgtcta tgacaaattg ctgttgatgg ttgacgctct
     1981 gggcaatcct cttgcaaccg atctcacctc taaaatgttt gcccactatc gagataaacg
     2041 cctgacaggc gagatctact tcagcgagaa atggaagaaa ggagcaagcc cggtcaccat
     2101 taacctagag caaagctatc taagtagtgt ttttagcgaa ctatcccgtc tgggcgaatg
     2161 gtcgtatccg aacccactgg agaacatgcg aaaattcacc atcgcagaaa aagagatggc
     2221 atggcttacc catgagcaga ttgttgaatt gctggctgat tgcaaacgtc aggacccaat
     2281 tctggcactg gtagttaaga tatgcttaag cacaggcgca cgctggcgtg aagccgtaaa
     2341 tcttacccgc tcacaggtga ccaaataccg aattaccttt gtcagaacga aggggaagaa
     2401 aaacagaagc atccctatca gtaaagagct ttacgaagag atcatggcgc ttgatgggtt
     2461 caatttcttc acagactgct attttcaatt tttatccgtg atggaaaaaa cgtctatcgt
     2521 gctccctcgc ggtcaactca cacacgttct gcgccatacg tttgcggcgc acttcatgat
     2581 gtcgggtgga aacattctgg ccttacaaaa aattctcgga caccacgata taaaaatgac
     2641 tatgcgttac gcacatctgg caccggatca tctagaaacg gcgctccgtt tcaatcctct
     2701 ggcaacgctg ccaagtggcg acaaagtggc ggcagcggtt ggcattaccc cgtaataacc
     2761 accactgacc accaacctaa cttattgttt ttaacctaac ttattgtttt cattaacccg
     2821 tttacataaa tgggtttttt gttgcctgaa atttatctac tacccagcaa tcctctcaac
     2881 catcctcaaa atctcctcgc gtgatagcgg cttacggtcg gtgacaaaat gcagcgtcat
     2941 tccctcaata aacgcatcaa gcgcgcgggc ggttccgggt tcaaaccatt gttcgagcgt
     3001 ttgttgactg cgctgcatcc agttttgcat taccgttttt aagagcggtt ttcggctagc
     3061 cagcgcgtag agctggtaca tcagctccat attatccggc gttgcgacct gtgagctgta
     3121 gatcatatcg gtgatagcct ggcatgcgcc tggagcatca ctaacatcgc taaaaaatgc
     3181 ctgatattgc cgggacatga tctcagtaaa acggctgaac gcctccagca acaactcatc
     3241 aattcctgaa aaatagtagg tcatcgatcc caacggcacc ccggcaaggg tagcaatttt
     3301 gcggtgtgta acagcatgta ttccgtaaag tttcaccgcc tccagcgtgg cctggataat
     3361 tttttctcgt cgttgcggat cgttagcgcg acgcatgtca tttcctctct ctgcaattgt
     3421 gtacaaatgt acacagtctt gctaatgttg tcttccctct tcttattttg acgtggtcta
     3481 tgaccgtaaa ttcttcacgt aatgcattga aacgccgaac ctgggcgctg tttatgttct
     3541 tctttttgcc aggcctgtta atggcgtcct gggcaacccg tacgcctgct atccgcgata
     3601 ttctctctgt ctcgatcgct gaaatgggcg gtgttctctt tggtctgtcg atcggttcga
     3661 tgagcggtat tctctgctcg gcgtggttag tgaaacgctt tgggacgcgt aatgtcatcc
     3721 ttgtcacgat gtcctgcgca ttgatcggga tgatggtatt aagtctggca ctctggctga
     3781 catcgccctt gctctttgcc gttggtctcg gcgtctttgg ggctagtttt ggttctgcgg
     3841 aagtggcgat aaacgttgaa ggtgccgccg ttgagagaga aatgaataag acggttttac
     3901 cgatgatgca cggtttttat agcctgggca cgctggcagg cgctggtgtc gggatggcac
     3961 tgacggcctt tggcgttccg gcaacgatgc acattttatt ggcggcgctg gtaggcatcg
     4021 cgcctattta tatcgccatt caggcaatcc ctgatggtac gggcaaaaat gctgccgatg
     4081 gcacccagca tggcgaaaaa ggcgttcctt tttatcgcga tatccagttg ctgttgattg
     4141 gtgttgtagt gctggcgatg gcctttgccg aaggttctgc caacgactgg ttacccttat
     4201 taatggttga tggtcacggt tttagcccta cttccggctc gctgatttat gccggtttta
     4261 ctctggggat gaccgttgga cgctttaccg gcggttggtt cattgaccgt tacagtcgcg
     4321 ttgccgtggt tcgggccagt gcactaatgg gggcgttggg tattgggctg attatttttg
     4381 tcgatagcgc ctgggtcgct ggggtgtctg ttgtactttg gggactgggt gcctcgctgg
     4441 gcttcccgct gaccatttct gccgccagcg ataccggccc cgatgcacca acccgcgtca
     4501 gcgtggtagc tacgaccggt tatctggctt tcctcgtcgg gccgccgctg ctgggctatc
     4561 tcggcgaaca ttatggatta cgtagtgcaa tgatggttgt actggcgctg gttattctcg
     4621 cggctattgt cgcgaaagcc gtcgccaaac ccgataccaa aacgcagacg gcgatggaga
     4681 atagttgatg agcattaaat taattgcggt agacatggat ggtactttct taagcgatca
     4741 aaaaacctat aaccgtgagc ggtttatggc tcagtatcag caaatgaaag cacaaggcat
     4801 tcgctttgtg gtcgccagcg ggaatcaata ttatcagttg atctctttct ttcctgaaat
     4861 tgctaatgaa attgcctttg tggctgaaaa cggcggctgg gtagtgagcg aaggcaaaga
     4921 tgtttttaat ggcgagctgt cgaaggatgc gtttactact gtcgtggaac atttgctgac
     4981 gcgcccggaa gtggaaatta ttgcctgcgg aaaaaatagc gcctataccc tcaaaaaata
     5041 tgacgatgcc atgaaaacgg tggcggaaat gtattatcac cgtctggaat acgtcgataa
     5101 ctttgacaac ttagaagata tcttctttaa atttggcctt aatctctctg acgaactgat
     5161 cccacaagtg caaaaagcat tacatgaggc catcggcgat atcatggtac cggtccatac
     5221 cggcaacggc agcatcgatc tgattatccc cggtgtacat aaagccaatg gccttcgcca
     5281 actgcagaaa ttatggggaa tagacgacag cgaagtggtg gtctttggcg atggcggtaa
     5341 cgatattgag atgctgcgtc aggcagggtt tagctttgca atggaaaatg ccggcagcgc
     5401 ggtcgtcgca gcggcaaaat accgggcagg ctccaataac cgtgaaggcg tactggatgt
     5461 gatcgataaa gtgcttaaac acgaagcgcc atttaagcaa taaaaaaggc agcacgcccg
     5521 gcaacacaat acctgaatga aggctgcata attcgcggaa attaacccgc tgcattattt
     5581 caggcgaaat gattatgaaa aattgtctac tgttgggcgc gcttttaatg ggctttactg
     5641 gtgtggcgat ggcgcaaagt gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg
     5701 ttcctgattc agtcagcgtt ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct
     5761 atgtggctcc tgcgccagct ccggcttacc gcccgcatcc ttatgttcgc catcttgcca
     5821 gcgtaggtga agggatggtt atcgaacacc agattgacga ccatcaccat taattcgaca
     5881 atgcctgatc gcacaaagca atcaggcatt tttttaccct tcgtgcgaat ttcccatctg
     5941 tttatctttt aaaaagataa ccatcagcga cagccacaaa atgccgttga caaggttgaa
     6001 gagattaaac agtccgttgc ccccgttcag ccaggcatgt ttgctgattt caataccgac
     6061 ggtaaagatc agcatttgca gcatccccat cgccgcagaa accgtacctt tactcatatc
     6121 gctggcaaac agggttaatc gcaccagtcc cgcattcgcc agaccaatac cgaaagcata
     6181 aatacttaac ccggcggtca tccataaata cgcgtgcgat gagataaccg ttgccgcagc
     6241 agcgaccaac agaccaatca taatcggcca gccgcccata ataatcagcg aacgtacggt
     6301 gcggcgcgag gtcagacgcg ctaacagcaa gttacccgca attaacgccc caaaaatagg
     6361 cacttgcagc aggccatatt catagctgct caactgctca ccggtaataa tgataatcgg
     6421 cgactgggcg atccacgcca gcaacggcag gctaacgaat cccagcgcca gcgcccccgc
     6481 cacaaagcga ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagcgacag
     6541 tttctcgcct atacgcgtgg cggtttcagg catagctcgt tgcagaccga aaaaggagat
     6601 cgctgccaat gcggcaaaca agacaaacat cccttcccag ggcagcacat ggatccacgc
     6661 cgcgcccacc agcggaccaa gtagtggagc aatcagcgcc acgttcgcca ttagcgcggt
     6721 gatcttgata caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc
     6781 aatgaaacag aggcttatac cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg
     6841 tgccagtaat attgccagac aggtgacgat aaaccacacc actcccgcca gcatcaccgg
     6901 acggcggcca atacgatccg acaacggccc caggagccat tgtaaaaaca tcccgcccgc
     6961 cagatacgca gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac
     7021 ggccaacata ccgggttgaa tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac
     7081 cagacagaga gggaaaagta acgcctgacg tccaagcctg gcaccggaag ctaatttatt
     7141 ttgcatgcaa tttcttcgcc aataataatc gcgcaaagtt taataaaagc gcagctaacg
     7201 agaaagcgaa ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat
     7261 agctacgctt tctttaagtt ttatttaacc tttgcccgtt acaatcacct accgtaaaca
     7321 ggccgcttga gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc
     7381 aacgacttgc ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa
     7441 atttgaatct ctctctattc tctcttatta acgcaacgcc agactctgcg ccgtggatga
     7501 tctcgttggc gatttttatt gctaaagatt tgattaccgt ggtgccgttg ctggccgcag
     7561 tactttggtt gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc
     7621 tggcggtcag cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct
     7681 ttgtcgaaaa tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg
     7741 atcacggtac ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt
     7801 ccggctcact tttaatggtg ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg
     7861 tccactggcc gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagcg
     7921 cccagattat ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc
     7981 gcttctgttt tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa
     8041 gtgtagtatt gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg
     8101 aagagcgtat cgggcagctg ctgcaagaat taaaacgcag cgataagtta catcttaaag
     8161 acgccgccgc cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca
     8221 gtgcgcccgt cgttttgctc ggcggctata ttgttctgga accacgcagc gccagccatt
     8281 acctgttaag cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg
     8341 ctgcgacgct ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt
     8401 ggattattga agcaattgat aatgaaatcc cttttaccgc cgtttgttat tcgctaaata
     8461 cttttctggc gctgaaagag aaaccccatt gccgcgcgtt tctttgcggt ggtgaatttc
     8521 acgccagcaa cgccattttc aaacccatcg attttcagca aacactgaat aatttttgcc
     8581 cggatatcgc tttttattcc gctgcgggcg tgcatatcag taaaggcgct acctgtttta
     8641 atcttgaaga gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg
     8701 ttgtcgacca cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct
     8761 ttgatattgt tgtgagcgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc
     8821 agcgtattaa gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt
     8881 atcagaagat taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc
     8941 ccacatccga ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg
     9001 ctcaatggat ttaccgttaa gctggaaatc aatggtgccg acaacctgac cttttttcag
     9061 cggtgcggta agctgcggtt ccgttaacgt ataactcgcc ttcaggtttt tcagctgccc
     9121 acgcggtatg gtcactgagc ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc
     9181 aaaccagacg cgctgagtca caaatgtggc atcaggttta attggcgtca cggtttcaaa
     9241 gaagcggaaa ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt
     9301 cgcccccagc actacggaga ttaaacgcat atcgccctgg gtagccgaag caaccagatt
     9361 atatcccgcg cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca
     9421 cagcagacga ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg
     9481 aatggcgtac tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg
     9541 cgcggtactg aactgccccg gcgcatccag gccgtgcacc gtctggaagg tagtgttggt
     9601 cagacccagt tttttggcat aaccattcat caaaccaata aatgactcct ggctcccggc
     9661 aacgtaatca gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt
     9721 caagtctgcc accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag
     9781 tgccggattt cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat
     9841 tttatcggcc ttaagcgcct gcccaaccac atagctggtc ataattttag tcaggctcgc
     9901 gggatccagt ttctcatccg cattgccttc tgccagcact ttaccgctgg cgtaatccat
     9961 taaaatccat gcacgggcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc
    10021 cgttggggca aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga
    10081 gtattgcgtc ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta
    10141 tgataagttc ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag
    10201 tttttaaagt ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag
    10261 ctgttgctta aaagttaact ttacttcacc atcggagtcc tgtttagcct gtggctgcct
    10321 gtcggaaaag gggtgagtat gattacgctg tggggtcgga ataattcaac caacgtaaaa
    10381 aaagtattgc tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt
    10441 gagtttggga taaatcacga tactgatttt ctggcgatga accctaacgg gctggtgccg
    10501 ttgttgcgtg acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat
    10561 ctggcggcac agtacgggca aaaacgcctg tggatcgact caccggcacg tcgtgcggaa
    10621 gcagaaaaat ggatggactg ggcaaaccag acgctcagta atgctcatcg cgggatcctg
    10681 atgggattag tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc
    10741 aaagagtgcg acgccctgtt tgccctgctc gatgcggaat tggcaaaagt aaaatggttc
    10801 tcaggcgacg agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt
    10861 aacgttggcc tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact
    10921 gaacgccccg cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact
    10981 ttaagtaatt ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg
    11041 cgaacatcac gaattcgttg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg
    11101 tcgccgttga cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt
    11161 tgctgccact gggggaattt gtcgctgtta tagaaagcca tgccgctcac ggcgggcgaa
    11221 tctttccagt aaaaaacagg ttgctcggtc ccggcgacga tctccccttt cgcttccggt
    11281 atcttaaagc ccgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct
    11341 ttttgcggaa tattaatttc atcaccaccg cgcgggccat gttcattcag ccatagtgca
    11401 ttactccacg gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg
    11461 gcgcgcacac cggattcctt tataaaagga ttatcatccg ggatttctcc caggtcggtc
    11521 agacgcacca gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt
    11581 tcgcccagag caataaaaag ataaccttta ccgtcaaata ccatccgccc gccaaaatgg
    11641 ttgccggtag acagttttgg catctgacga aagacggtgc ggaagtcggt cacttttgag
    11701 agatcatcac ttaagcgacc ataacccaca gcagttccgg ctttgccatc atcgccaact
    11761 tcggaataac ttaaccagat gcggcgcgac tgagcaaaat cgggcgctaa aaccacgtcc
    11821 agcaggccgc cctgcccgtg cgcccaaacg tccggcactc cggaaagcgg cgcagataat
    11881 ccttttcctg cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga
    11941 ttatcgggta aaaaggccag cgcccaggga tggtcgagtt tgtcttgcag tacttcgaca
    12001 tttaccgttg caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa
    12061 aaggattgtc gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag
    12121 gagagaacaa attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc
    12181 ttagttttca ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta
    12241 agctcatcag acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat
    12301 ggtgcccact catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag
    12361 cactccactc ttcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc
    12421 gattcagttt aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt
    12481 ttgccttcct cagttgcacg tatgcggcga ggtcaacgcg tgcgtcgatc aaccggttca
    12541 gcaggtcgat tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt
    12601 gcaattaaat agtatggctg ttttttgagc aaaatgctgt ccaggatcaa ttcttcacgc
    12661 cgaattaaca aaatttacat ttccttcaac tccgccagcg aatgagattg cgatacaaat
    12721 tttatccaga actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa
    12781 gctatgagca aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgcccgaaa
    12841 aaccttgtcg attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta
    12901 ccgagctatg acgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg
    12961 gtacaagaat cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg
    13021 accggttgtc tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa
    13081 atcaccgggc ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa
    13141 ccgaaacaca acccgttcct gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt
    13201 cattatgctt atctgaaaat ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt
    13261 ccgtctatgc gcggcgatct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa
    13321 cgtctggttg atgcgggcgt taaagaaatt ctggtgatct cgcaggatac ttccgcgtat
    13381 ggcgtcgatg ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg
    13441 gtcagcctgt gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttac
    13501 ccttatccgc atgtggacga cgtcatccca ctgatggcag agggcaaaat cctgccgtat
    13561 ctggacattc cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt
    13621 tctgtagatc gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc
    13681 ctacgctcaa cctttattgt tggcttccct ggcgagacgg aagaagattt ccagatgcta
    13741 ctcgacttcc tgaaagaagc acgtctggat cgcgttggct gctttaaata cagcccggtt
    13801 gaaggtgcag acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc
    13861 tggaaccgtt tcatgcagct gcagcagcag atttccgccg agcgcctgca agagaaagtg
    13921 ggtcgtgaaa ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc
    13981 atggcagatg caccggaaat cgacggcgcg gtttatctca atggtgaaac caacgttaag
    14041 ccgggtgata tcctgcgagt gaaagtcgag cacgccgatg agtacgattt gtggggtagc
    14101 cgggtttaat taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact
    14161 gccagctaat ctgcgccctg ccgtttttct tactcttata cagccgctca tccgccagca
    14221 ttttcaatcg ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta
    14281 aattaatgct tcccacctgg aaatgaacga tttcttttag aatcgatagc aactcttcca
    14341 gcgtgccgcc agtataaatc accgcaaatt cgtcacctcc gaagcggtag actttacctt
    14401 tatctaccgg gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca
    14461 gcaaatgacc gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat
    14521 aaccgccttt ttccggcagc gcttttaaat cttcgtcaaa ggattttctg cccatcgctt
    14581 tggtcaggtc gtccaggaat gccgagttat acatactgta gagttttttg acatcggcag
    14641 ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt
    14701 tctccacaat accacgatta atcactctgc gtaccagata gaggcaactg gcacccgata
    14761 tcactgccac caatgcggca aagaaaataa tcaatgtaca tacctgaaaa taaacgtctt
    14821 ttttataagc aaatacgtac aatccggtcg gcaaatagga gaagtgctcc attgcttccg
    14881 aaacggtgaa gatggcgttt ttagcgttat attcatcacc ggtcaccgat ttaccgttct
    14941 tgaacaggga gaacatcagg ttctcgctat ccatcgccag cgattttgac agtaaagaaa
    15001 aacgttccag accaagcaaa aatatcagac tacgattgtg agttaccgga tagaggtaat
    15061 agacgaaggt tttaccatca aaagagataa accctgtatt tttgacatgt cgatacttaa
    15121 caacctgcat aatatctgca caataatttt tctcaaactc ctttacctga atacattcgt
    15181 cactggaaat cagataagga ccgacatcgt acaaataaga gggtgaaacc gagtcggcgt
    15241 aagctgtgcg taagccaacc aacagctgtt cctccgtagg atttagcgtg ttgatcagat
    15301 caattgccac actccgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa
    15361 cggactgttt caggccatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa
    15421 ctgctgaaat catgataaaa atcagcagac tgactgtaag tacgaattta ttgattctgg
    15481 acatacgtaa actactcttt tactaatttt ccacttttat cccaggcgga gaatggcatg
    15541 ggtttctggt acagataccc ctgagcctga acacatccta atttgattaa ttcacgcgca
    15601 acgtcttcgc tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccagatcg
    15661 aaaataatgc gcacaatttt tcccgcagta ccgaaaatat cgccggtaaa ttgcgaatcc
    15721 acctttatac tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca
    15781 aagtcatcaa tcgatacccg taccccttgt ttacgcagtt gttcaatatt gagatagaaa
    15841 tgttcattaa ttcgctcgat cacggtttcg gtgatctcaa cacaaaggct gttcgccttc
    15901 aggccatttt ctttcatgat ggtagtaaaa cgctcatgaa agtgtggttc gtttaactgc
    15961 cagggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa
    16021 tgtctgaatt cacccatcgc ggttttaata atttgatagc ccagctcatt gataaacccg
    16081 atatcttcag caatggtaat gaacttcagc ggtgaaataa tcccccgctc cgcagacacc
    16141 caacggcaca acgcctccgc gcccaaaatg gtgcgggtgt taatatcaac aatcggttga
    16201 tagaccagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata
    16261 ttctcttcta tttcgttgag tttcgcatca tcacagaaaa taaactctga gaacatatgg
    16321 ttgcggatac tggaaatcgc cagtttcagc ttttctgcgt aactccaggc gttttccccc
    16381 tgcaaaggtt cacaaatagc agacttatgg atgcgcagat tgcggtagcc aaaactgctg
    16441 tcaaaatcgt ttaccgtcgc tatcttacgg gtaaacgtct gtacattttc ccggcacagg
    16501 agtaaataga gatcggcatt aaatgtcacg atatcgcagc attgcgcata tttctcggca
    16561 attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc
    16621 acagcttcca gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac
    16681 tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat cagcgagagc
    16741 aatttatttg cccgttcaat ttcagcatct ttcatttccg cgatcgaatt tttgatgctg
    16801 ataatctctt tcgcatcgcc ttcacgaatc ttcagggcct cgatttgatc cgtagagtca
    16861 ggcaaaaacg ccagcgtatt gaccaaatct gtcatttcag aaaattgacg acggaaaact
    16921 cgagtggtca ttaaataaac caccagcaat acgataataa tgaaagcaaa cggcagataa
    16981 aaaagtttat ctgatgcgtt caataaatta ccaccaggat aataaaaata gatttcgaat
    17041 aatgcgttgt ttggcaccgc tgatgttttt tcaatatatt ccgtatcgac agaatcaata
    17101 atattataat taccttcgct attataaata atcaattttt cacgagcgag ttgattctta
    17161 gcaatgatct catgaccttg ctttaaaaat agaacttcat cattagcgct gatgtgatca
    17221 agatactcgt tcagatatcg ccagtttaaa tcgacatgaa ttttatctac cgctaacaaa
    17281 tcatgatctt tgccgttgat gtgtagcgta aaagagatac atttttaccc ggacgatcaa
    17341 agcaattgta tgccgggttc cagctgttgt tagagtcatc tgaagccatc gaccacggtc
    17401 gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat
    17461 ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat
    17521 atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttca
    17581 gaatcacggc acgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg
    17641 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
    17701 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atgatgaaaa
    17761 aaagaccaat aaatgacaat gctgccaata aaaataaaat tatcagcctt atctttatct
    17821 tttcgtataa actcagcatg ttaatacatt taaaaaaata actgtctgaa cgctgtgccg
    17881 ctgcaaaata gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc
    17941 agaaagttac aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc
    18001 aatattcaaa cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc
    18061 caacaaattg aacgccagta cggtcagaaa aatagccagg gccggaaata cagcgacatg
    18121 cggcgcgata accatatccg ctcgtgcctc attgagcatt gcaccccact ctggtgtcgg
    18181 cggctgcgca ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat
    18241 gcgcatggtg aaaaacacca cgatagaaga gacggtccca ggcaagatat gacgcaacaa
    18301 aatggtcata tcgctggcac caatactgcg tgctgactcg ataaaggttt gctgtttcaa
    18361 caccagcgtg ttgccgcgca ccagccgggc aaacgcgggg atggaaaaaa tggcgactgc
    18421 gataatcacg ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat
    18481 acccgggaag gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc
    18541 ataatatcca gccagcaagc ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa
    18601 cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc
    18661 cagtgaatcg acaccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc
    18721 aaaataattt tcggcgtcat agggagcgat ccagcgtgca aaaatggcca ccacaatcaa
    18781 taaaataacg aacaatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa
    18841 ttcatgccac ggtgtacgta cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc
    18901 ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg
    18961 gcgtaaagca catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt
    19021 tccgcctgaa tcactgggta atcgcgcatt tctacggagt caacgagtaa gcgtccaagt
    19081 ccgggccagt tgaaaacttt ctccaccacg atggaaccac cgagcaagaa gccaaactgt
    19141 aagcccatca tggtcactac cgggatcatc gcgttgcgta gcccgtgttt gaggacaacc
    19201 caggtttcgc tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacgtcg
    19261 acaaacgacg cgcgggtaaa gcgcgccatc acggcggcca ctgccgcgcc gagcgtcagg
    19321 gagggtaaaa tgtagtgctg ccaactgtct gctcccacgg taggcagcca gcccagttca
    19381 acggagaata cctgaattaa aagtatcccc agagcaaatg ccggaaacga gatccccgac
    19441 accgcaatgg tcatactcaa tcgatccggc caacggttac gccagacggc ggcgataatt
    19501 cccgccgcca taccaaatat caccgcccag accatactgg ttatggtcag ccagagcgtt
    19561 ggcataaagc ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca
    19621 aaatccccct gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc
    19681 aaccccaact gctgacgcac cagttctata acctgcgcat cagcttcggg cccggcaatc
    19741 aatcgcgccg gatcgccggg cagcatatgg acaaataaaa acaccagcac cgagacgata
    19801 aacagcgtcg gaatcaaccc cagtaagcgt ttgataacgt aattaagcat tccactccct
    19861 gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa
    19921 aaccggtcag gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg
    19981 gcgactcttg ccagatgata tcctgcgccg ccttatacaa gcgggtcttt tccgccggat
    20041 cattagtttt cagtgcctga gccaggaagt catccacctg tttattgctg taaaacgcgg
    20101 tattaaacag cgttggtggc cagttctgcg aggcaaacag cggcgataga gcccagtcgg
    20161 cttcgccggt tgaagccgac cagccagtgt agaacatccg cacgccgctc tctttttgcc
    20221 ctttaccttc aacttctgcc gcccgctgtc cggcatccat cgcagtcacc tgggctttaa
    20281 tcccaacctg cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt
    20341 tatgtgacga ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt
    20401 cgcgcgcttt cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatactcg
    20461 gcggtaccac accagtagct ggcgttgcat agcccgcgaa ggccactttc accagcgccg
    20521 ggcggttaat ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg
    20581 tcacgttcat actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt
    20641 tgtttttctc cagcagtgcg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac
    20701 cggtttgcag cattgccgcg cgggtgttgt tatccgccac cggacgccag gttatgctgt
    20761 ccagtttggg caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg
    20821 tctgattcca ggtatccagt tcatacggcc cggttcccac cggatgaaaa ccaatctcct
    20881 tgccatattt ttccagcgct gccggtgata tcatcgcggt cgccggatgg gcaagaatat
    20941 taataaacgc tgagaatggc tgtttgaggg taatctttac cgttgtcgga tcgatcgctt
    21001 ccgttttggc gatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg
    21061 cccggtccag attcgctttc accgccgctg cgttgaaatc ggtgccatcc tggaatttaa
    21121 tgccttcccg caatttcacg gtgtaagtaa ggccgtcatc ggaaacggtg taactctccg
    21181 ccagcacgtt tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt
    21241 tcgctacggc ctgagataac gtgtcatttg cgtcatacgg atcgagcgtg gtgaagttcg
    21301 atcccaccgc caccaccacc tctttggcag cgaatgcaca agatgccatc aacgctgtcg
    21361 caatgcccag cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct
    21421 gaatgttatc tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc
    21481 ccgacgcatt gcaacgagac ggctgcaacc tcttcgccac gcagatgaat attgctggga
    21541 agatcgtccg acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact
    21601 gccgccagta atttacgcgt ataaggatgc tgcggatttt caaagaccgc gcgccgtgga
    21661 ccaatttcaa caatttgccc gagatacatc accgccacac gatgactaat ccgctcaacc
    21721 acggccatat cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc
    21781 aacaagttga taatctgccc gcgaatagaa acatccagcg ccgagacggc ttcgtcggca
    21841 atgatcactt ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg
    21901 ccggaaaact catgtgggta acgccaggca tgttccggta acaggcccac gcgctccagc
    21961 aaccacgcaa cgcgtgcagc cgcttcttta cctggcaata aaccgtgtac gcgcagcggt
    22021 tcgagaatcg aatcaccgat agtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa
    22081 ataaactgaa tatcgcggcg caatgcctga agtttaccgg gtgacaaggt atcgattcgc
    22141 tgaccgttaa agataatttc gccgccctgc gattcgacca ggcgcaacaa cgcccgcccg
    22201 gtagtggatt taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga
    22261 tcaaaactga ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg
    22321 cgcaaaggga aacgggtgac cagattacgc acccgtaaaa caggttcgcc atccaccacc
    22381 gttttctgct cgatggatga ctcctgtttc gctggatgtt caagcgatat caatgggaaa
    22441 cgtcggggat aatctaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca
    22501 cgggtataag gatgttgcgg tgcatgaaaa atctgttcga cgctacccgt ttccaccgcc
    22561 tcgccctgat acatcaccag tacccgatcg gcaatctctg ccaccacacc catatcgtga
    22621 gtgataaaga taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc
    22681 tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg
    22741 cggcatgaca gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc
    22801 ggataacgtg aaagaatggt ttgtgcctcc ggaatgcgta cctgatccag catccgcttc
    22861 gcctcgacca tcgcttcttc acgactggcg ttctgatgca gacgaataga ttcagcaatc
    22921 tgttcaccca cagtaaatac cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc
    22981 atatccgcac cgcgcacatg gcgcatttgt gcagcgctct gctcgctaag ttcaattact
    23041 tcgcgactgc gccgctgcaa cagcatttta tcgcactgca ctaaaccgcc cgcctgttcc
    23101 aacaggcgca tcaatgccag cgcagtcact gacttaccgg agccggattc gccaacaatt
    23161 gccagcgtct caccacgttg caggctaaaa gagagattgc ggaccgcagc tattttctgc
    23221 tgctcctgca taaaggcaat attcagattt tcaaccgcca gcacatcacc ggcatcaagt
    23281 tcatcactgt gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc
    23341 gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc
    23401 ttcatggtcg atagcgatta agccaccgct accgccaagc gcagggagtt tttccattac
    23461 tacccgctcg caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatatc
    23521 atatgccgcc agcgcgcgga tgaagacttc gcccgtaccg gtacaggaaa ccgccacact
    23581 ggcgttattg gcatagcatc cggcccccac taagggacta tcgccaactc gtccgggtaa
    23641 tttattggtc attccgcccg tggacgtggc tgccgccaga ttgccgtcta aatccaacgc
    23701 cacggcccct acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag
    23761 gactgttgcc ccttcctcgc gcgctgccat tagttgttca taacgcaaag gtgtggagaa
    23821 aatctccggc gagacgcgct ccatgccatg agcaaacgca aaattttctg ccccttcgcc
    23881 aatcatcatc acatgcgggc tttgctccat caccagccgg gcggcaagaa ccggattacg
    23941 cagatgacta acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcagacaggc
    24001 gtccagttca tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg
    24061 acactcttcc agcagacgca ccgcttccgt caccacatcc agcgcacttt cgcccgctac
    24121 cagcattttc tgcccggttt caacaatggc agacaacgcc tcgatgtagc gtaattcctg
    24181 ttgcagactc atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc
    24241 tttgcccata gtattcgtcc attaagagtg gctcgccact tttgctataa atatcagaat
    24301 ttttatgaat gactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca
    24361 ggagccagcg ctacgcccga ggtggcatac agtctgtggc gtttttctga cataataggc
    24421 aaattcgatt ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc
    24481 tcgacaccgc gcttaatgag atgctttctc gcgttacccc actgaccgcc caggaaacgc
    24541 tgccactggt tcagtgtttt ggccgtattc tggcgagcga tgtcgtttcg ccactggatg
    24601 tcccggggtt tgataactcc gctatggacg gctacgcggt gcgtttagcc gatattgcct
    24661 ccgggcaacc gctgcccgtt gctggtaaat cctttgccgg tcagccgtac catggtgaat
    24721 ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag
    24781 cggtggtgat gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag
    24841 cgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt
    24901 tcccggcggg gactcgcctg actaccgctg aactgccagt gattgcttcg ctagggattg
    24961 ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc
    25021 agttgcccgg tcagccgctg ggcgacggcc aaatctacga taccaaccgt ctcgccgtac
    25081 acctgatgtt agaacagttg ggatgcgagg taatcaactt agggattatc cgcgacgatc
    25141 cccatgccct gcgcgccgca tttattgaag ccgacagcca ggcggatgtg gtgatcagtt
    25201 ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac gattcttgaa gagctggggg
    25261 agatcgcctt ctggaaactg gcgattaaac cgggtaaacc gttcgcgttc ggtaaacttt
    25321 ctaatagttg gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc
    25381 aactggtaca gcctttgctg gcaaaactaa gcggcaatac cgccagcggc ctgcccgcgc
    25441 gccagcgtgt acgcacggca tcgcctctga agaaatcgcc aggacgcctt gatttccagc
    25501 gcggcgtgct gcaacgcaac gccgatggcg aactggaagt gacgaccacc ggacatcagg
    25561 gttcacatat atttagctcc tttagcctcg gcaactgctt tatcgtgctg gaacgcgatc
    25621 gcggcaatgt ggaagtgggc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag
    25681 gcctgtaatg gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct
    25741 gcgcggtttt gattttgacg gtcaggaagc attgaaagat tctcgcgtgc tggtcgtcgg
    25801 ccttggtgga ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct
    25861 gacgctgctc gacttcgaca cggtttcgct ctccaatctg caacgccaga cgctgcacag
    25921 tgatgccacg gtcgggcaac caaaggtgga atccgcccgt gacgccctga cgcggatcaa
    25981 cccacatatc gcgattacgc cagtcaatgc gctgcttgat gacgctgaac ttgctgcgat
    26041 gattgcaaaa cacgatctgg tgctcgactg tacggataac gttgcagtac gtaatcaact
    26101 gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga
    26161 agggcaaatc acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg
    26221 tttgtttggc gagaacgcgt taacctgcgt agaagcaggc gtgatggcac cgttaatcgg
    26281 cgtaattggt tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc
    26341 agccagcggg aaaatcgtga tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact
    26401 gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaactg ccacctccag gccagataag
    26461 acgcggcact ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg
    26521 acgttctgcc aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcatcaaccg
    26581 ccggatagct aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacaccctg
    26641 ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact cgcggctaac actttcgcct
    26701 gcggcgcatg cattttcaat aactggtgta agtcggtcac agtctgaata ccgctaccgc
    26761 cctgagcatc aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca
    26821 gtagcccttg tgctgcgcca tataccgcgg ttcccagcgt cggaatccct tccgctttta
    26881 acatcttaat agctgccagc ccctctgcgg tcaccggaac tttcaccacg atatccgcaa
    26941 taatagaacg caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct
    27001 gggcaaacag acgcccctga ccgcccatcg cctcgtgaag ttgcggaagc acaacctcca
    27061 gcggtttttt acccactgcg ataatgcttg ggttagtggt tacacccgcc agcggaaaaa
    27121 tacgtgacag cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca
    27181 tcctcaaaat gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt
    27241 gacgcacatc aatccaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg
    27301 caactatgat tttcaatatt caacgctact cgacccatga tggccccggt atccgcacgg
    27361 tcgtatttct taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg
    27421 cccgcacgca ggatctgctg tatgacccac gactctgtct ggaaggctgt gagctgtgcg
    27481 ctaagaccgc accggaagtg attgagcgcg cgttgaatgg tttgcttatt catcgggaaa
    27541 agttaacccc ggagcatttt acggcgctaa ccgactgctg cccgacacag gcgttaaccg
    27601 tgtgtggtga agtgaaaagc gttgaggaga tcatggcaac cgttttgcgc gataaaccgt
    27661 tttacgatcg cagcggcggc ggcttaacac tttctggcgg agaacccttt atgcagccgg
    27721 aaatggcgac agcgctactg caagccagcc acgaagccgg tattcatact gcggtagaaa
    27781 cctgtctgca tgtgccgtgg aaatatatcg ccccttctct gtcctatatt gatctgtttc
    27841 ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg
    27901 ccagggtgct ggataatctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg
    27961 tgccactgat tcagggcttt aatgccgacg aaacctctgt gaaagccatt accgattttg
    28021 ccgccgacga gctgcacgtt agcgaaattc atttcctgcc ctaccacacg ctgggcatca
    28081 acaaatatca cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag
    28141 aactgctcga ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag
    28201 gataacaacc atgaccacac tgaaactgga cacgctcagc gatcgcatta aagcgcacaa
    28261 aaatgcgctg gtgcatattg tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac
    28321 cgagatgtat caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc
    28381 gcatcacctg gcgaatcgca ccatctggat caaacatgat gaattgatca ttggcaacca
    28441 ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat accgtctcct ggatcgaaaa
    28501 agagattgat gatctggcag atcgtcccgg tgctggtttt gcggtgagtg aagagaacaa
    28561 acgcgttctg catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta
    28621 cggcatgttt accgatgagc aaaaaggtct gctggcgacc ggcatcatta aagcggaggg
    28681 caatatgacc tccggcgatg cgcacctggc ggtcaatttc ccgctactgc tggaaaaagg
    28741 tcttgatggt ctgcgtgaga aagtggcgga acgtcgctcg cgcatcaatc tgacggttct
    28801 ggaagatctg cacggcgagc aattcctgaa agcgattgat atcgtgctgg tggcagtcag
    28861 tgaacacatt gaacgtttcg ctgccctggc gcgtgaaatg gccgcgaccg aaacccgcga
    28921 aagccgtcgc gatgaactgc tgacgatagc agaaaactgc gatcttatcg cccaccagcc
    28981 gccgcagact ttctggcagg cgctgcaact gtgttacttc atccagttga ttttgcagat
    29041 tgaatctaac ggtcactcgg tatcgtttgg tcgtatggac cagtatctct atccatacta
    29101 tcgccgtgac gttgaactga accagacact ggatcgcgaa cacgccatcg agatgctgca
    29161 tagctgctgg ttgaaattgc tggaagtgaa caaaatccgc tccggctcac actcaaaagc
    29221 ctctgcggga agtccgctgt atcagaacgt caccattggc gggcaaaatc tggttgatgg
    29281 tcaaccgatg gacgcggtaa atccactctc ttatgcgatc ctcgaatctt gcggtcgcct
    29341 gcgttcgact cagcctaacc tcagcgtgcg ttaccatgcg ggtatgagta acgatttcct
    29401 cgacgcctgt gttcaggtga tccgctgcgg cttcgggatg ccggcgttca acaacgacga
    29461 aatcgtcatc ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc
    29521 agcaattggt tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat
    29581 gagctttatc aacttcgccc gcgtgatgct ggcggcgctg gaaggcggtc gtgatgccac
    29641 cagcggcaaa gtgttcctgc cacaagaaaa agcgttgtca gcgggtaact tcaacaactt
    29701 cgatgaagta atggacgcat gggatacgca aatccgttac tacacgcgta aatcaatcga
    29761 aatcgaatat gtcgtcgaca ccatgctgga agagaacgta cacgatattc tctgctcggc
    29821 gctggtcgat gactgtattg agcgagcgaa aagtattaag caaggcggcg cgaaatatga
    29881 ctgggtttct ggcctgcagg tcggcattgc taacctcggc aacagcctgg cggcagtgaa
    29941 gaaactggta tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga
    30001 tgacttcgac ggtctgactc acgagcagct gcgtcagcgg ctgattaacg gtgcgcctaa
    30061 gtacggcaac gacgatgata ctgtcgatac gctgctggct cgcgcttatc agacctatat
    30121 cgacgaacta aaacagtacc ataacccacg ctacggtcgt ggtccggttg gcggcaacta
    30181 ttacgcgggt acgtcgtcta tctccgctaa cgtacccttt ggcgcgcaga ctatggcaac
    30241 accggacggg cgtaaagcgc ataccccgct ggcagaaggc gctagtccgg cctccggtac
    30301 tgaccatctc ggcccaacgg cggtcattgg ctcagtaggt aaactgccta cggccgcgat
    30361 tctcggcggc gtgctgctca accagaaact gaatccagca acgctggaga acgaatctga
    30421 caagcagaaa ctgatgatcc tgctgcgcac cttctttgaa gtgcataaag gctggcatat
    30481 tcagtacaac atcgtttccc gcgagacgct gctggaggcg aaaaaacatc cggatcagta
    30541 tcgcgatctg gtagtgcgcg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga
    30601 cgctcaggac gatatcatcg cccgtactga acatatgctg taatcttctc agcccggcgg
    30661 cggcgtcgcc gggctaaatc attcacatca tcaatttcag cctcgctttc attcgaaata
    30721 taatttgtgc tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga
    30781 gcacacctat gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg
    30841 ccaaaacgta caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca
    30901 ttgagttcgt tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc
    30961 taaaggatga gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc
    31021 agttgttcca cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa
    31081 aagataagca actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg
    31141 cccccgaagc attcgtcgca ctgatggcaa aacactacca tcgcctgaaa cctgtaaaag
    31201 attatcagga gattgacgac gtactgttca agttttcgct caacctgccg gatgaacaaa
    31261 tcccgttagt gatcgacaaa ctgcacataa cgctggatgg cattatgaaa cccgtcacca
    31321 gtggttttgg ctttatcgac ttgattattc ccggtctaca taaagcaaac ggtatttcgc
    31381 ggttactgaa acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta
    31441 acgatgcgga gatgctgaaa atggcgcgtt attcctttgc gatgagcaat gctgcggaaa
    31501 acattaaaca aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg
    31561 tgattcaggc ggtgctggaa aataaaccgc cgtttaatct ttgattatca ccaattcagt
    31621 ttattgagaa agtgcatgtc attataccgg atacggcgtg accgccttat ttggcataca
    31681 aaatcatgaa aattcaatat attgcgatac actaccaggc ctgataagcg tagcgcacca
    31741 ggcaatcttg cgtttgtcat cagtttcagt agccctgacc atatcagggc tactgataat
    31801 aacttactcg atccctttac tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac
    31861 gcgttccggg gtgatttcca gaatacgggt cgccagggag cttacaaact cacggtcgtg
    31921 agaaacaaag atcagcgtgc cctgatacag ttccagcgcc atgttcagcg actcaatgga
    31981 ttccatatcc aggtggttgg tcggttcgtc catgatcaga atgttcggct tctgcatcat
    32041 taacttacca aacagcatcc gccctttttc cccaccggaa agcactttag ctggcttttt
    32101 gatgtcgtcc tggctgaaca gcaaacgacc gagaatactg cgcaccgcct gctcgtcatc
    32161 gccttcctgc ttccactgac tcatccattc gaacacggtc agatcatttt caaactcata
    32221 ttcgtggtcc tgagcgtagt aaccaatgcg cgcgttttca gaccatttta cggtgccgct
    32281 gtccggttgc agatcgccca ccagcgtttt cagcagcgtt gatttaccga cgccgttggt
    32341 acccagtacc gccagttttt cacccacttc cagcagcagg ttgagatttt taaacagtgg
    32401 accgttatca aaccctttag tcagaccttc cacttctagc gcgttacgga acagtttctt
    32461 atcctgttca aaacggatga acgggttctg acggctggaa gctttcacct cttccagttt
    32521 gattttatca atctggcgcg cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct
    32581 aaagcggcta acgaaagatt gcaactcagc aatctgcgct ttcttcttcg cgttatcagc
    32641 cagcagacgt tcacgcgcct gagtcgccgc cgtcatgtac tcatcgtagt tgcccggata
    32701 aacgcgcagc tcgccgtaat ccagatccgc catgtgggta caaaccatgt taaggaagtg
    32761 acggtcgtgc gagatgatga tcatggtgct gtcacgctcg ttcagcacct gttccagcca
    32821 gcgaatggtg tcgatatcga ggttgttggt cggttcgtcg agcaggagaa tgtcgggatc
    32881 agcaaacagc gcctgagcca gcagcacacg cagcttccag ccaggagcaa cttcactcat
    32941 cgggccgtag tgctgttcca ctggaattcc cacgccaagc aacagttcac cagcgcgagc
    33001 ttccgcagag taaccgtcca tttcgccgta tttcacttcc agatcggcca ctttatagcc
    33061 gtcttcttca ctcatttccg gcaaagcata gatgcggtca cgctcctgct tcacttccca
    33121 caactcttta tgccccatga tcaccgtatc cagcacagtg aactcttcaa aggcaaactg
    33181 atcctgacgc agcttaccaa tgcgttcgtt gggatcgagg gaaacgttac ccaacgtcgg
    33241 ctctaagtcg ccgccgagga tcttcataaa ggtggattta ccactaccgt tcgcgccaat
    33301 caggccgtaa cggttgccgc cgccgaattt gacggaaatg ttttcaaaca acggcttact
    33361 gccgaactgc atggtgacgt tactggaaac taacacaggc gtatcctgaa aagagatatg
    33421 acaaaccgcg cattatgcca taagtctgaa agaagatcct gctcctcgct taaggtctac
    33481 actcaagcca aaggcaaaaa atgtgatttc gtacacatct gatttcactg tgagctggaa
    33541 tgaacttata atgcgcttcc agtactctaa tattctcaac ccaatggcct gccaggcaca
    33601 aaatctcgct taacatgaat atgaaattga aaacattatt cgcagcggcc ttcgctgttg
    33661 tcggcttttg cagtaccgcc tctgcggtaa cttatcctct gccaaccgac gggagtcgcc
    33721 tggttggtca gaatcaggtg atcaccattc ctgaaggtaa cactcagccg ctggagtatt
    33781 ttgccgccga gtaccagatg ggactttcca atatgatgga agcgaacccg ggtgtggata
    33841 ccttcctgcc gaaaggcggt actgtactga acattccgca gcagctgatc ctgccggata
    33901 ccgttcatga aggcatcgtc attaacagtg cagagatgcg tctgtattac tatccgaaag
    33961 ggaccaacac cgttatcgtg ctgccgatcg gcattggtca gttaggcaaa gacacgccta
    34021 tcaactggac caccaaagtt gagcgtaaaa aagcaggccc gacctggacg ccgaccgcta
    34081 aaatgcacgc agagtaccgc gctgcgggcg aaccgcttcc ggcggtcgtt ccggctggtc
    34141 cggataaccc gatggggctg tatgcactct acatcggtcg cctgtacgct atccatggca
    34201 ccaacgccaa cttcggtatc ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg
    34261 aagacatcaa attcctgttc gagaaagtac cggtcggtac ccgcgtacag tttattgatg
    34321 agccggtaaa agcgaccacc gagccagacg gcagccgtta tattgaagtc cataacccgc
    34381 tgtctaccac cgaagcccag tttgaaggtc aggaaattgt gccaattacc ctgacgaaga
    34441 gcgtgcagac agtgaccggt cagccagatg ttgaccaggt tgttctggat gaagcgatca
    34501 aaaatcgttc cgggatgccg gttcgtctga attaatcttc aaaacgtaaa gcaaaggcgg
    34561 actcataatc cgccttttta atttgacaga ccttatcccg ccgggatcat aaccaacaaa
    34621 acatatccca ccagcgccgc ccacaacagc atcggtatcg aataaagatg aaaacgccac
    34681 cagatacggc gatcgttcgc catccgtaac gcaatcaaat ttgccagcga tccgggtaat
    34741 aaaccaaagc cacctacgtt taccgcccat gccagtaata aagacggtgg cacataattg
    34801 agcagcaaaa tggtactcgg cacgttactg atcacctgcg ataaaccgat cgccgttagc
    34861 cataatccgg gttcggataa atgactcacg ttacttaaca ccccctgcaa aactggcagt
    34921 tgggttagta aatggacgtc gataaacatc gccataaaca ccagcagcag cgtccagtcc
    34981 acactgagca ccacgcgacg cgccagcagc gcaaagcccg ccgccacaat caccagtccc
    35041 cacagctctt gttttaactc cagcgccgtc agaaagacga tatacagccc cagacaactc
    35101 cacaccagcc gcggtttcca ctccggcgtt tgccctcctg tatggtactg cagcgccttt
    35161 ccagggaaac aacaccagca caacagcagg agtgtcagca tcattgcgcc agccagcggt
    35221 gccatttggg caataaatcc ggcaaacgaa agaccagaac gtccccagat aagaatattt
    35281 tgcgggttgc caattggcgt cagtagcgaa ccagcattga ctgccagcgc ctcaaaaata
    35341 atcagccgat taaccgggat ctcacacagt ctttttagcg tgatagtcag cggaacaact
    35401 ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca gcgccgccgc cagtaccata
    35461 aacatcgcca gccgacgctc cgtagcaaaa cggcgcacca ttttgcgccc cagcacatca
    35521 aaataaccgc ttaactcgac acctttagtc agcagcatca ggccgcttaa ggtgatgatg
    35581 gtgtgccagt cgatagcagc aggccaggat ttcggtgcaa agggaacgaa aaaacttaat
    35641 ccgatgccaa caagaattaa taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa
    35701 ggcaggctca tttggcaccg tgtttctggg taaacaaacg aaaggcatcc agcgtctctt
    35761 cactaacatg gtgctccata ccttccgcgt cgcgacgggc gatttccgga ctgacgccca
    35821 acaccaacaa gaaattttcg actatctgat gtcgctcgcg gctttcctgc gccagcttct
    35881 ctccttctgc cgttaaaaac acgccacgcc aggggatcat ttcaatcagc cccatggttg
    35941 ccagtcgctt aagcatttta gccaccgtcg gttgcgaaac ccccagacga gcagccatgt
    36001 ctacctgacg agcttccccc acttccctga tcaagtcaga aatcagctca acataatcat
    36061 caataagctc gcgccgatgc gcctctctga cctggcggaa cccttcaacg tgctcttcca
    36121 cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc tgcgcgacga ctcattatgc
    36181 ttcctcatta tggtgacgca tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga
    36241 gttgcgaagg tacaaaaaat taacgtttta gcaatagcta tataatatag cctgtgctat
    36301 atctgtatgt aatgcaatca tccctcaagg atcgatggga ttagcaagtc aggaggtctt
    36361 atgaatgagt tcaagaggtg tatgcgcgtg tttagtcatt ctccctttaa agtacggtta
    36421 atgctgctct ctatgttgtg cgatatggtc aacaacaaac cgcagcagga taaaccttcc
    36481 gataaatagc ggcgtcgcgg tacgccgctt cactcctgct tttatgcagg cacaacgcgt
    36541 tttggtctga aaaaccccac tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt
    36601 cgtcggtagt tgacctgaac agcagctcgc tctatcttct tgcagccctg cgtatattgc
    36661 ggctcgcgga tgcggacccc tttcccctct tcacgcactc ttgcaggtat tgacccttga
    36721 cgccagggta agcacatggc gtttgttacg atagtggcat atgaatttaa ccctcaaaga
    36781 atcgcttgtt acccgtagcc gggtatttag cccgtggact gcgttctact ttttacagtc
    36841 gctattaatt aacctcggct taggttaccc cttcagtttg ctctacaccg ctgcgtttac
    36901 ggctattttg cttttgctat ggcgaacatt gcctcgcgta caaaaagttc tggtcggtat
    36961 cagttcgctg gtggcggctt gttatttccc ttttgctcag gcctacggcg cgcctaactt
    37021 caatacattg ctggcattgc actccaccaa tatggaagag tcgaccgaaa tcctgacgat
    37081 ttttccgtgg tacagctacc tggtcggcat atttattttt gcgctcggcg taatagcaat
    37141 caggcgaaaa aaagagagtg aaaaagcgcg ctggaatacc ttcgacagcc tgtgcctggt
    37201 attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac ctggcctggg gcggcgtatt
    37261 taaactgaaa gatactggct atccggtatt tcgttttgct aaggatgtca tcgtcaataa
    37321 taacgaggtg attgaagagc aagaacggat ggcaaaactt tccggaatga aagatacctg
    37381 gacggtcact gccgttaagc cgaagtatca gacctatgtg gtggtgatcg gagaaagcgc
    37441 gcgtcgcgat gccctcggtg cctttggcgg tcactgggac aataccccgt ttgccagcag
    37501 cgttaacggt ttgatatttg ctgactacat tgccgccagt ggctccacgc agaaatcgct
    37561 tggcttaacg ctcaatcgcg ttgtcgatgg caaaccacag tttcaggata actttgtcac
    37621 cctggcaaat cgcgcgggct tccagacctg gtggttttcc aaccagggtc aaatcggcga
    37681 atacgatacc gccatcgcca gcatcgccaa acgagcagat gaagtgtact tcctgaaaga
    37741 aggtaatttt gaagcagata aaaacacgaa agacgaagcg ttactggata tgaccgctca
    37801 agtgctggcg caagagcact cgcaaccgca gctgattgtt ctacatctga tgggctcgca
    37861 tccgcaggcc tgcgaccgga cacaaggaaa atacgaaacc tttgtgcaat cgaaagaaac
    37921 gtcgtgctat ctctatacca tgacgcaaac agacgattta ctgcgcaagc tgtacgatca
    37981 gttacgcaac agcggcagca gcttctcgct ggtttacttt tctgaccacg gtctggcctt
    38041 taaagagcgc ggcaaagacg tgcaatacct tgctcatgat gataagtatc agcaaaattt
    38101 ccaggtgcct tttatggtca tttccagcga cgataaagcg catcgggtga ttaaagcccg
    38161 ccgctcggcc aatgacttct taggcttttt ctcgcagtgg acgggaatta aagcgaagga
    38221 aataaatatc aaatacccgt ttatatctga aaagaaagcc gggccgatat acatcaccaa
    38281 cttccagtta cagaaggtgg attacaacca tctcggaacc gatattttcg acccgaaacc
    38341 ttaaaacaaa aaaatccgcc ccgagaggcg gatttttata tcaccgaagt gattagaagc
    38401 ggtaaccaac accggcaatc caggtgccta cgtcaacgct acgaatacgg ctctgctcgt
    38461 aagagaagtc cagagcaacg ttttccatcg ggttgaactg cagaccagca ccgtaggaga
    38521 aaccgtagtc gctggtgtcg tgtttgtagg tcgggtattc agtggtctgg aatttaccat
    38581 aacccacacc cactacaccg tagatgcttg cccagtcgtt aatgcggtaa gccggaccag
    38641 cagtgatgcc gtagtactgg tttttgttgt agtcaccaga gcttgcagta cggcttttct
    38701 cggtgtaagt gaaagaaccg atcacaccca gcgggctgtt gtcttcttca tagcggtatt
    38761 tcaggttgaa accgcccatt ttgttcattt ggccctgagc gtcgctctgt gcgtaaccgc
    38821 cagttacagt agaagtcgca gctacggaag tacctgcggt gaaagccaga actgcggcca
    38881 gtgctgaaag acatgcaatt tttttcataa ccacctcaaa tgtgattcaa ataagtccta
    38941 agttttaaat atatcaaaaa ttaatgggaa actcttcgcg atttatgatg tctaacgggc
    39001 catttcatgt aacagaacgt ttccatacac cgctatccat ctaaatttaa atcacttttt
    39061 cagagaactg cgtaagtatt acgcatattt tccctgtcat tcatccagat tattcctaat
    39121 caccagacta atgattccat caatcctggc gcattttagt caaaacgggg gaaaattttt
    39181 tcaacaaatg ctcaaccagc attgggtata tccagtacac tccacgcttt acttaggttt
    39241 agatatttgt gggagaaagg atgcctggtt cattacgtaa aatgccggtc tggttaccaa
    39301 tagtcatatt gctcgttgcc atggcgtcta ttcagggtgg agcctcgtta gctaagtcac
    39361 tttttcctct ggtgggcgca ccgggtgtca ctgcgctgcg tctggcatta ggcacgctga
    39421 tcctcatcgc gttctttaag ccatggcgac tgcgctttgc caaagagcaa cgattaccgc
    39481 tgttgtttta cggcgtttcg ctgggtggaa tgaattatct tttttatctt tctattcaga
    39541 cggtaccgct gggtattgcg gtggcgctgg agttcaccgg accactggcg gtggcactgt
    39601 tctcttctcg tcgcccggta gatttcgtct gggttgtgct ggcggttctt ggtctgtggt
    39661 tcctgctacc gctggggcaa gacgtttccc atgtcgattt aaccggctgt gcgctggcac
    39721 tgggggccgg ggcttgttgg gctatttaca ttttaagtgg gcaacgcgca ggagcggaac
    39781 atggccctgc gacggtggca attggttcgt tgattgcagc gttaattttc gtcccaattg
    39841 gagcgcttca ggctggtgaa gcactctggc actggtcggt tattccattg gggctggctg
    39901 tcgctattct ctcgaccgct ctgccttatt cgctggaaat gattgccctc acccgtttgc
    39961 caacacgcac atttggtacg ctaatgagca tggaaccggc tctggctgcc gtttccggga
    40021 tgattttcct cggagaaaca ctgaccccca tacagctgct ggcgctcggc gctatc
//
LOCUS       DAJOTB010000025        38621 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJOTB010000025 DAJOTB010000000
VERSION     DAJOTB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.025"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            208..1863
                     /gene="aslA"
                     /locus_tag="OHN28_001344"
     CDS             208..1863
                     /gene="aslA"
                     /locus_tag="OHN28_001344"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HCQ2296777.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1954..3189)
                     /locus_tag="OHN28_001345"
     CDS             complement(1954..3189)
                     /locus_tag="OHN28_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2296778.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPELNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3336..3412)
                     /locus_tag="OHN28_001346"
     tRNA            complement(3336..3412)
                     /locus_tag="OHN28_001346"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3376..3378),aa:Pro,seq:tgg)
     gene            complement(3455..3541)
                     /locus_tag="OHN28_001347"
     tRNA            complement(3455..3541)
                     /locus_tag="OHN28_001347"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Leu,seq:cag)
     gene            complement(3562..3637)
                     /locus_tag="OHN28_001348"
     tRNA            complement(3562..3637)
                     /locus_tag="OHN28_001348"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3602..3604),aa:His,seq:gtg)
     gene            complement(3696..3772)
                     /locus_tag="OHN28_001349"
     tRNA            complement(3696..3772)
                     /locus_tag="OHN28_001349"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3736..3738),aa:Arg,seq:ccg)
     gene            complement(3875..5260)
                     /locus_tag="OHN28_001350"
     CDS             complement(3875..5260)
                     /locus_tag="OHN28_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2296779.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5451..6191)
                     /gene="rffM"
                     /locus_tag="OHN28_001351"
     CDS             complement(5451..6191)
                     /gene="rffM"
                     /locus_tag="OHN28_001351"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCQ2296780.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6194..7546)
                     /gene="wzyE"
                     /locus_tag="OHN28_001352"
     CDS             complement(6194..7546)
                     /gene="wzyE"
                     /locus_tag="OHN28_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCQ2296781.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7543..8622)
                     /gene="rffT"
                     /locus_tag="OHN28_001353"
                     /gene_synonym="wecF"
     CDS             complement(7543..8622)
                     /gene="rffT"
                     /locus_tag="OHN28_001353"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCQ2296782.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8619..9869)
                     /gene="wzxE"
                     /locus_tag="OHN28_001354"
     CDS             complement(8619..9869)
                     /gene="wzxE"
                     /locus_tag="OHN28_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCQ2296783.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9871..11001)
                     /gene="rffA"
                     /locus_tag="OHN28_001355"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9871..11001)
                     /gene="rffA"
                     /locus_tag="OHN28_001355"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCQ2296784.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11006..11680)
                     /gene="rffC"
                     /locus_tag="OHN28_001356"
                     /gene_synonym="wecD"
     CDS             complement(11006..11680)
                     /gene="rffC"
                     /locus_tag="OHN28_001356"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCQ2296785.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11658..12539)
                     /gene="rfbA"
                     /locus_tag="OHN28_001357"
     CDS             complement(11658..12539)
                     /gene="rfbA"
                     /locus_tag="OHN28_001357"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCQ2296786.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12558..13625)
                     /gene="rffG"
                     /locus_tag="OHN28_001358"
     CDS             complement(12558..13625)
                     /gene="rffG"
                     /locus_tag="OHN28_001358"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCQ2296787.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13625..14887)
                     /gene="wecC"
                     /locus_tag="OHN28_001359"
     CDS             complement(13625..14887)
                     /gene="wecC"
                     /locus_tag="OHN28_001359"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCQ2296788.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14884..16014)
                     /gene="wecB"
                     /locus_tag="OHN28_001360"
     CDS             complement(14884..16014)
                     /gene="wecB"
                     /locus_tag="OHN28_001360"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCQ2296789.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16070..17116)
                     /gene="wzzE"
                     /locus_tag="OHN28_001361"
     CDS             complement(16070..17116)
                     /gene="wzzE"
                     /locus_tag="OHN28_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCQ2296790.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17128..18231)
                     /gene="wecA"
                     /locus_tag="OHN28_001362"
     CDS             complement(17128..18231)
                     /gene="wecA"
                     /locus_tag="OHN28_001362"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCQ2296791.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18471..19730)
                     /gene="rho"
                     /locus_tag="OHN28_001363"
     CDS             complement(18471..19730)
                     /gene="rho"
                     /locus_tag="OHN28_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCQ2296792.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19815..19916)
                     /gene="rhoL"
                     /locus_tag="OHN28_001364"
     CDS             complement(19815..19916)
                     /gene="rhoL"
                     /locus_tag="OHN28_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCQ2296793.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20057..20386)
                     /gene="trxA"
                     /locus_tag="OHN28_001365"
     CDS             complement(20057..20386)
                     /gene="trxA"
                     /locus_tag="OHN28_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCQ2296794.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20517..21782
                     /gene="rhlB"
                     /locus_tag="OHN28_001366"
     CDS             20517..21782
                     /gene="rhlB"
                     /locus_tag="OHN28_001366"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCQ2296795.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21918..23402
                     /gene="gppA"
                     /locus_tag="OHN28_001367"
     CDS             21918..23402
                     /gene="gppA"
                     /locus_tag="OHN28_001367"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCQ2296796.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPPQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFEQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFANRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23449..25470)
                     /gene="rep"
                     /locus_tag="OHN28_001368"
     CDS             complement(23449..25470)
                     /gene="rep"
                     /locus_tag="OHN28_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCQ2296797.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25557..25838
                     /gene="ppiC"
                     /locus_tag="OHN28_001369"
     CDS             25557..25838
                     /gene="ppiC"
                     /locus_tag="OHN28_001369"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCQ2296798.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25884..27359)
                     /gene="ilvC"
                     /locus_tag="OHN28_001370"
     CDS             complement(25884..27359)
                     /gene="ilvC"
                     /locus_tag="OHN28_001370"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCQ2296799.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27509..28399
                     /gene="ilvY"
                     /locus_tag="OHN28_001371"
     CDS             27509..28399
                     /gene="ilvY"
                     /locus_tag="OHN28_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCQ2296800.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28396..29940)
                     /gene="ilvA"
                     /locus_tag="OHN28_001372"
     CDS             complement(28396..29940)
                     /gene="ilvA"
                     /locus_tag="OHN28_001372"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCQ2296801.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29943..31793)
                     /gene="ilvD"
                     /locus_tag="OHN28_001373"
     CDS             complement(29943..31793)
                     /gene="ilvD"
                     /locus_tag="OHN28_001373"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCQ2296802.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31858..32787)
                     /gene="ilvE"
                     /locus_tag="OHN28_001374"
     CDS             complement(31858..32787)
                     /gene="ilvE"
                     /locus_tag="OHN28_001374"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCQ2296803.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32807..33070)
                     /gene="ilvM"
                     /locus_tag="OHN28_001375"
     CDS             complement(32807..33070)
                     /gene="ilvM"
                     /locus_tag="OHN28_001375"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCQ2296804.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33067..34713)
                     /locus_tag="OHN28_001376"
     CDS             complement(33067..34713)
                     /locus_tag="OHN28_001376"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCQ2296805.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34716..34766)
                     /locus_tag="OHN28_001377"
     CDS             complement(34716..34766)
                     /locus_tag="OHN28_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296806.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34853..34951)
                     /gene="ilvL"
                     /locus_tag="OHN28_001378"
     CDS             complement(34853..34951)
                     /gene="ilvL"
                     /locus_tag="OHN28_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCQ2296807.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35304..36824
                     /locus_tag="OHN28_001379"
     CDS             35304..36824
                     /locus_tag="OHN28_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCQ2296808.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36849..37187)
                     /gene="maoP"
                     /locus_tag="OHN28_001380"
     CDS             complement(36849..37187)
                     /gene="maoP"
                     /locus_tag="OHN28_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCQ2296809.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37306..38145
                     /gene="hdfR"
                     /locus_tag="OHN28_001381"
     CDS             37306..38145
                     /gene="hdfR"
                     /locus_tag="OHN28_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCQ2296810.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38241..38316)
                     /locus_tag="OHN28_001382"
     tRNA            complement(38241..38316)
                     /locus_tag="OHN28_001382"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38281..38283),aa:Trp,seq:cca)
     gene            complement(38325..38401)
                     /locus_tag="OHN28_001383"
     tRNA            complement(38325..38401)
                     /locus_tag="OHN28_001383"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38365..38367),aa:Asp,seq:gtc)
     gene            complement(38456..38571)
                     /gene="rrf"
                     /locus_tag="OHN28_001384"
     rRNA            complement(38456..38571)
                     /gene="rrf"
                     /locus_tag="OHN28_001384"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9435 a        10559 c         9736 g         8891 t
ORIGIN      
        1 gcgatataac atcaccgcct tacatatcac ctgcgccaga ggtaggattg aaaacgctct
       61 cctgattttc caattcattt tctggataaa taaataattt atttttatca ccattattta
      121 tgcaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac gtcatcattt
      181 tgacgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa acttcttgtt
      241 gttgctgtcg ccgcagctct tcctctcata gccagcgcag cagatacccc gtcaaccgcc
      301 accgcacgca aaggctttgc cggatacgat cacccaaacc agtatctggt taaaccggcg
      361 accactattg ccgacaacat gatgccggtg atgcagcatc ctgcacaaga caaagaaacc
      421 cagcagaaac tggcggaact tgagaaaaaa accggtaaga aaccgaacgt ggttgttttc
      481 ttgctggatg atgtgggctg gatggacgtt ggctttaacg gcggcggcgt ggcggtgggt
      541 aaccctacac cagatatcga cgccgttgcc agccaggggc tgattttaac ttcggcgtat
      601 tctcaaccga gttcttcccc aacccgcgcc acgattctca ccggacaata ctccatccac
      661 cacggcattc tgatgccgcc aatgtacggg caaccgggcg gtctgcaagg gttaaccacg
      721 ctgccacagt tgctgcacga tcagggctac gtcacccagg ctatcgggaa atggcatatg
      781 ggggaaaaca aagagtcgca gccgcagaac gttggctttg atgatttccg cggctttaac
      841 tcggtatctg atatgtatac cgaatggcgt gatgttcatg tcaacccgga agtcgccctg
      901 agcccggatc gttctgaata catccaaaaa ctgccgttta gcaaagatga cgttcatgcc
      961 gtgcgcggcg gtgaacagga agccattgcc gacattacgc cgaaatatat ggaagatctg
     1021 gatcaacgct ggatggaata tggcgttaag ttcctcgaca agatggcgaa gagtgataag
     1081 cctttcttcc tctattacgg cactcgcggc tgtcacttcg ataactaccc gaacgccaaa
     1141 tatgcgggta gctctccggc gcgcacctct tacggcgact gcatggtgga gatgaacgat
     1201 atcttcgcca atctgtataa agcactggag aaaaacggtc agcttgataa cacgctgatt
     1261 gtgttcacct ctgataacgg tccggaagcc gaagttccgc cgcacggtcg cacgccgttc
     1321 cgcggtgcga aaggttctac atgggaaggc ggcgttcgtg tgccaacctt cgtttactgg
     1381 aaaggcatga tccaaccgcg taaatctgac ggcattgtcg atctggcgga tctcttccct
     1441 acggcgctgg atctggcggg gcatcctggg gcgaaagtag cgaatttagt accgaaaacc
     1501 acctttatcg atggtgtgga ccagacatct ttcttcctgg gaacaaatgg tcagtctaac
     1561 cgtaaggccg agcattactt cctcaacggt aaactttctg ctgtgcgtat ggatgagttc
     1621 aagtatcacg tcctgattca gcaaccttac gcttataccc agagcggata tcagggtgga
     1681 ttcaccggca cagtaatgca aacggcggga tcgtcggtgt ttaacctgta caccgatccg
     1741 caggaaagcg actctatcgg cgtgcgccat atcccaatgg gtgtaccgct acagaccgaa
     1801 atgcatgcgt atatggagat cctgaaaaaa tatccaccac gcgcgcagat taaatctgat
     1861 taagccggcg ctatcgctga gatctacctt tgccggatgc gatgctgacg catccgacaa
     1921 tcaaccgcag gcggccacaa aatggctgct tttttactta ttcaccacca gcaaatgcgc
     1981 ctgcataatg tcgctggccg gacgaccgtg cgccagcaaa tcaaccattg ctttaagata
     2041 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gctcgggttt
     2101 gccgctggca tcgagcataa agcggtgttt cgggcagcct ccccagcacg cttttaacac
     2161 gttgcaactg cgacactgtg ccggtaactg cttgaattta tcttcaccaa atgcctgctg
     2221 ttgcggggaa tcaatcattt ctgcaatcat ctgctgaagc atattcccta gtcgatactg
     2281 cggataaaca tagtgatcgc aggcataaac gtcaccgttg tgctcaacaa tcaccgagcg
     2341 cccacaggtt ggctgatgat ggcaaaccgc gcccggtgcg ccgacaaaat tggcgaacgc
     2401 ccattcgata ttcattacga aaatcttgcc gacgtcgcgc ttaatccagt ggtcgaaaat
     2461 cgccaccaga aactcaccga actcttgcgg gcacaccgac cattccgtca gttcgccctg
     2521 aatatcgccg ggagcatgta gcttaagtcc agcatgagca gccgtttcat tagccaggcg
     2581 ctcgaccacc ggaataaact ggatgaactc aacacctgca tcacacagaa aatcatagac
     2641 ttgcaaaggt tgtaacgcac tggtgcgatt gacgcagacc agcacgttat agtcgacatg
     2701 atgtttttgc aggagcgtca gggcacgcat caccagctta tgcgtgggtc tgccaccttt
     2761 ggtcacgcga tattgattgt ggatctcagc cggaccatcc agcgataacc ctacaagaaa
     2821 atgattttct gccagaaatc cacaccattc gtcatcgagc agcacgccgt tagtctggaa
     2881 gctattactt atcttcctgc cagcaccata tttcgcctgt agcgccacgg cacggcggta
     2941 aaactccagc ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     3001 ggtttcgctg gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg
     3061 cgtgactggc ttttcgtggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     3121 cagattacaa tcagaaccgc tcggtttcgc catcacatga aaagcacgcg ttggaacctg
     3181 ttgctgcatg gtcgctcctt gcctgaagtc atccttcaat aagttaaggg cgaaatcata
     3241 ccgtggcgca atgcgttatt acgggcgttt attgcgaagg gaaagtgacg aaatgcacag
     3301 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
     3361 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
     3421 ctttttgaat ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc
     3481 ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg
     3541 cattgtcaca acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa
     3601 tcacaatcca gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt
     3661 attactacca ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc
     3721 tgagacctct gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc
     3781 gttgcggggg tggataatac ggacttccca cccctctgtc tagtcccttt ttaaataaat
     3841 tgcgcgtttg gttacggttt gtgcgttttg ctgcttattc ctccagttta tgcgctttac
     3901 cgtggcgatt aagcccaaaa actttataaa tcgccgtcac cgtcagcata aagatgatgc
     3961 cgacaaacag cgacatacgc gtatcttcat taaagtacat gccgatcaaa acgcaaatca
     4021 ggaatgccat tgttacgtag ttggcccaag ggaacaggat tgagcggaac ggatggctgg
     4081 caattgccgc tttatgcgca cgacgaaaac gcagctggct aatcaatatc acaaaccatg
     4141 gcaccatccc cggaagcacg ctggcactgt agacatagac aaacacacgc tgcggattgg
     4201 gaatgatgta gttcaggcat gagccaatta gcagaatagc aatagatacc gccacacccg
     4261 caaccggtac gccgtgacgg gaaactttcg ccatcgccgc cggtaactga cggtttttcg
     4321 ccagtgcata gagcatacgt ccgcaactgt acatgccact gttacagcca gaaagcgctg
     4381 ccgtcagcac cacaaagtta ataatgcccg ccgcagcggt aatgccgatt ttggcgaaag
     4441 tcagtacgaa cgggctgccg ttgctgccta tttcattcca cgggaagatg gtaacgataa
     4501 cgaaaatcgc gcctacgtag aaaatcagga ttcgccacag caccttgcct acagcgctac
     4561 gcagcgtcac ctgcggattc ttcgcttcac cggcagtaat gccaatcagc tccacgccct
     4621 ggtaggacgc caccacaata cacaatgcgg tcaggaaccc tttccagcca cccgcaaaga
     4681 aaccgccatg ctctgtgaga ttgctaaaac caatcgactg cccgccattg ccaaagccaa
     4741 agaaaatcac gcccaggcca atgacaatca tcacgataat cgtggtgact ttgatcatcg
     4801 cgaaccagaa ctcgatttcg ccgtacaacc tcaccgcagc cagattcgcc aacgccacca
     4861 gcgccaccgc gatcaatgcg ggtatccact gcgccatctc cgggaaccag aactggacat
     4921 aaacgccaat ggcggtgatt tctgagatcc ccaccgccat ccacataaac cagtaagacc
     4981 aggcggtgag atagccaaag aacgggctca tataacgatg cgcgtaaacg gcgaacgaac
     5041 cggtaaccgg ttcgaggaac aacatttcgc ccattgaacg catgatgaaa aagacgaaca
     5101 gcccggcgat gatataggcc aacaatacgg atggcccggc ccatttcagg gtactggcgg
     5161 cccccataaa caggccgacg ccaatggtgc cccccagggc gatgagttcg atatgtcgag
     5221 cttccagccc acgctgtagc tctggtttgt tatctgccat aaatcctcgt gttgtgtttg
     5281 catgctttcc ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg aatgttttcg
     5341 taattcagga gaaatggcaa ataaagcatt aaaaatttga atgctttgtg taataaaaaa
     5401 gcagacaggc gacggagtga ccacttcgtc gctttacaaa gagaggaaaa tcagaggttg
     5461 ccggtatagt gccagcgtaa ataacgcagc aaacgaagct gacgcttaat gcggctcggc
     5521 tgcgaaagca ggcggtagag ccactccagc cccagcgttt gccagatttt cggtgcgcgt
     5581 tttacgtgac cggtgaaaac atcgtaggtc ccgccaacgc ccatatacag cgcatctgga
     5641 tgcaccagac ggcagtcgcg catgaagatc tcctgctttg gcgatcccat cgcaacggtg
     5701 acgatttgcg ctccgctggc atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta
     5761 aaataaccat cctggctgcc aacgatattc acattccact ggttgcgcag tttagcttca
     5821 gtttgcgcca gcacttcagg tttaccgccc acaagaaata ccggtgtccc ttctttgcct
     5881 gcgcgcgcca tcagctcttc ccagagatcg gccccagcaa cgcgggaaac ctgcgcctgc
     5941 gggtactttt tacgtactga acgtacaacg cttatgccat ccgcatattt aaattcggca
     6001 gcgttaatta gctcccggac ctcggcgtta tcttcaatgg tcagcatttt ttcggcatta
     6061 atggcaacca gcgttccttg cttaagctgc ccgtcagcga acagataatc gagggcgtgc
     6121 tgcatatcac gccaaccaat caactgtaag ccacgcagcg tataggtcgg tgccgtggtg
     6181 ttgttattca ttattatcct tcaacctgcg tccggagcga tgattttgtt cgtttatgaa
     6241 tgagtccggc gctttcaaaa agccagtaca acagttttgc gatcatcaga catgcgccga
     6301 agaccacgat aaaaaagacc acgcgtgaga caaacgaatc cagcccttca cgcgccagca
     6361 cgatcatatt gaagatcgcc ccgaagcaga aactgtgcaa tatcgcagcc ttatagcgat
     6421 tagtctcgcg gttgcccagc tcatacagcc agtcgaacca tttgatgatc agtccaacaa
     6481 caatcgcccc gagcgggatg aacaacgcgc cgcccatcac caccagtgag cctataagcg
     6541 taggcgagat cgccagtccg gagtggttat tcagcacttc ccaggtaaag tagttggcgg
     6601 agttcagcac catactcggg cgacccggcc acagccagga agggataaag acatagaaat
     6661 cgcggacaat tggagccagg ccctggaagt cgatgttgtc gtagttctgc aacagcaacg
     6721 ccagattctc ccacggtgaa aaggtgtcgc gagtgagata gagaaacgta tagaacgctt
     6781 catcgccgct cacattcatt ccatagcgtt ttaacgccag ccagaacatg cccacaatcc
     6841 ccagcacgcc cgccgccgcc agcatccaca acgaaatcca gccgcgaata atgccaataa
     6901 acaggaagat agcgaatgcg atgatgatat tggcgcgagt gccgccgaca atcatataag
     6961 tcagcaagcc aaaagcgacc gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc
     7021 gcagaaagta gaccaccagc atcgccggga tgaaaaagta aaagaagcgt tttaacgcca
     7081 cgccggagac ttcactggag aagatctgac tgtaggagtt aagccggaac agcaaaaagc
     7141 cgttgtgcat gaagaagatg ccgacgctta ccagcgcgat gcccatcagg atcacccacg
     7201 tgagattggt ttccacgcgg ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc
     7261 gtttgcgtag gcgggttttg taggtgacgt agtaaaccgc gtagaagcag cccgcagaaa
     7321 gcaacgcctg caacaagatt tctggcggcg cggcaccaac atcaaagcga aaaaccagca
     7381 cgctggtcag cgggaagccg aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat
     7441 tgaagttaaa gcgcacacgg cgaaactcaa accaagtcag cgtggcaata aacagcgtgc
     7501 agagcagcca gacaacaaac aggccactga attgcagcag actcatgcga cctccccggc
     7561 ggcaatcgcc aacgcccgct gccagccttg cagatagttc gggctaaaga aggcaatggt
     7621 gtttttatcc accgacacca actggcgctg cgcttcacgc acaatatcct cgttgagatc
     7681 gtcggtagta aacagcaccg gcaaatgctg ttccgtcata tcctgccaga acggattttc
     7741 ccggttaagc acacaaggaa tgcccgcctg aatcagtaag cacagcgtac caatgccctg
     7801 ctggcgggca aaaataaagt aaccaagatc gcactgacga agtagcgcca gataggcgtc
     7861 aaattccagt ttttcgctca gagtttgtag attttcttcg ctgaataact ccagccccgc
     7921 ctgacgaact tcctcgatgt acgcttcgtt attaggcgga tatcccatcg gcaccaccac
     7981 ttttaccgta tcgccaaatt gctgataaac ggcgcgcaag gcagcaatat gttcattgct
     8041 gcggtcaccg gagttcccca ccagaatggt cattttccct tcacgttgcc gatcattcgc
     8101 catcatattg agtgaagggt ccatccgcgt cgggaagtac agcagttctc cccgcacctt
     8161 tggatgcgtt ttggcaaaaa agctcaaatc accgcgggtg gcaaatacac agccgactct
     8221 cttttgcgcc aggcgacgta gcgggtaaaa aagcttatat ctcaagccac tggaaagctc
     8281 gtacaggtct gccccccaga tatgccagta aaactggctg ggcttaatgc caccactcag
     8341 cagagccagc cacagtgtgg gattgaactg accgtggaag aaaaaacgct gctgacggtt
     8401 agcttttgct ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga aaaattgcac
     8461 agaaagcgcc ggacagctat cacttaagcc gtcgtccttg ccaacaacca taaactcgcg
     8521 cgcgtgctcg ctcgtcgcgg ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg
     8581 gtgagggata tccgatccca gtacgtgaat cagtacagtc atgcccgcct acgccagagt
     8641 aaaaacacgc cacaacaaag agaaaaatag acgatatagg ttgccatata ggcctgcgcc
     8701 gcgcccagtg caccatgcgc agggattagc cagtgggcaa ataccatcaa taaagtgaac
     8761 tggctgactt ccgccagaat ataaaaccgc agtgacgctt tggcgatcac cagataacca
     8821 aagacataag cgcccacttt taacacatca cccaccaact gccaggcaaa gagatcgcgc
     8881 atagcggtaa atttattcga caacagcagc cagatagcaa aatcacgcag caaccagacg
     8941 gtaaaactcg ccgccgccac tgccggcaag acgaatttaa gcgatttaac cacttcccgg
     9001 gtgatatcgc gcttttccgt tagccgcgac aacgtgggca gcaaatatac gctgaacgat
     9061 gccgtaataa attgcaggta ggcatcggaa atactgctca ccccttgcca gatccccacc
     9121 tcatcccagc tatactgcgc cgccagcagt ttacgcatca tgatgtaagc aacaggcaag
     9181 gtcaccgacg taatcaacgc catgagcgta aatttgctca actgccctgc cagaccgtta
     9241 tcccagctgg gtttcagata gcttaacggg atgacaccac gtttgatcaa catgatggcg
     9301 gcaggaatta ccaccagcgc gggaatcagc gccagaccca gcaacgcccc ttcataaccg
     9361 cccaaacggt aactgacgta gtacgcgaga acgccaatca agctgccaac aatcagagat
     9421 aacgcattac ctgcggcatc gcgaaagcct ttcatcagcg ccagtaacag gttgccccag
     9481 gcgatcccca tttgcaccag cgccactaaa cgcaccagcc cctgatagtc ggtattacca
     9541 aacaatccct ggctgattgg cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag
     9601 aaaccaagta ccatcgctga tgaagtgccg accacgcggc gcagctgttg cggattatca
     9661 tggtactggg caacgtattt ggttacaccg ttaaagatgc cagccccggc aagcacgccg
     9721 agcacggtaa tcaactggcg gaaatttgcc gccagcccaa gccccgccgg accaaatgac
     9781 accgccagca acttaccgac tagtaacccg gcaccaatct tgaccagtgt actggccgcc
     9841 gtccacaagg acgcttttgc caacgacata tcaggagaag tagttcaaca aagtcgcaat
     9901 taccgtacgc tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc
     9961 gctctctttg gtggtgtagc gatcttcacc gtggaactca ccaaagtgtt cccccgcagg
    10021 gcaaccgtgc agcggaatgt aatgaaacac cgccatgatt tccgcttctt tcagaaagtt
    10081 aatcaacgcg ctccggtcat caatatcccg cagtttaatg tagaacatat gcgcgttctg
    10141 cacgcagcca tcgggaatcg acggcagctc gatacgccca ttttttgcca gaggcgccag
    10201 tgcatcgtag tagttttgcc acagcgccag acgctgctga ttgatacgat ccgctgcttc
    10261 cagttgcgcc cacaggtacg ctgcttgcag atcggacatc aaatagctgg agccaatatc
    10321 gcgccaggta tatttatcga cctgaccacg gaagaactgg ctgcggtttg tgcctttttc
    10381 acggatgatc tcggctcgtt cgatcaacgc tttatcgtta atcagcgtcg cgccgccttc
    10441 accacccgcc gtgtagtttt tggtttcatg gaagctaaag cagccaatat gaccaatggt
    10501 tcccagcgca cgccctttgt aagtggacat cacgccctga gcggcatctt ccaccacaaa
    10561 tagattatgc ttcttcgcca acgccataat agtgtccatt tcgcaggcca cacccgcgta
    10621 atggaccggc acgataacgc gcgttttgtc ggtgatcgcc gcttcaatca gcgtttcgtt
    10681 gatgttcatg gtgtccgggc gaacatccac aaagacgatt ttcgcgccac gcagcacaaa
    10741 ggcattggcg gtggagacaa aggtgtagct cggcatgatc acttcatcgc caggctggat
    10801 atcgagcagc agcgccgcca tctccagcga agcggtgcag gacggcgtca gtaacacttt
    10861 ggcgctgcca aaacgttgct ccagccactg ctggcagcga cgggtaaaac caccatcgcc
    10921 gcacagtttg ccgctaccca ttgccgactg catatagtcg agttcggttc ccaccaccgg
    10981 cggtgcgtta aatggaatca tgtgatcacc tgtataacca gtacgcggtg ctttctacat
    11041 tcgcaccact ttgtatgtat cgtttaagcg cggcggtgtt gcccatctgg gtcgccaccc
    11101 gcaaagttgt tttaccgcga gcatacgccc agtttagcgc cgtttgcatt agctcagcac
    11161 ctgcaccgcg tccagccagc aggccaattc gcgcatctgt cgcattgagt tcccgtaaag
    11221 agacataacc gcgaatatcg ccggacgccg cacgtaaaat cagacattga tgatcaaagg
    11281 tgccgcgcac ggcattttca atccactgtg cataaaagcg accgctggcg tcaggcgcat
    11341 accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg
    11401 gaatatcggt ctcttgtgcc actactgcac cgctatcact gacattgttc acgggtagtg
    11461 ccaaatcaac ttcaccttct atcagggaga atcccagctg ttgcagagca tccagttcac
    11521 ccgtatttga tgccgcaatt ttggcctgca cccgtgacca cggcgctaag gcgtctggcg
    11581 tcaggagtgc cgcgtcaggg ctaatacgca caatggcgct gttaacacca aagaaggcgt
    11641 tttcccaggt tagtggctca atactggcgc ggacgggcac gaagtaactc cagcagatat
    11701 tggccgtagc cagttttcgc taatgaactg gcagcacgct tcacaccctc gtcatcgagc
    11761 cagccgttac gccaggcaat ctcttccagg caggcaatct taaagccctg gcgtttttct
    11821 actgtctgca caaaagtgct ggcctcaatc aggctgtcgt gagtgccggt atccagccag
    11881 gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg cctcgaggta catctggttg
    11941 atggaggtaa tctccagttc accacgctcc gacggcttca cctgctttgc gtactccacg
    12001 actttactgt cgtagaaata aagcccggtc accgcccagt ttgatttcgg ctgttttggt
    12061 ttttcttcca gcgagatagc gcggaaattg tcgtcaaact ccaccacgcc aaagcgttcc
    12121 gggtccatca cctgatagcc aaataccgtc gccccttccg tgcgcgccgc aacatgacgc
    12181 agtttcggac tgaagccctg tccgaagaag atgttatcgc ccagcaccag acaagaaggt
    12241 tcaccgttga ggaaggtttc accgatgata aaggcctgcg ccaggccgtc cggactgggc
    12301 tgttcggcat attccagctg gataccgaac tcactgccgt cacccagcag gcgctggaaa
    12361 taacctttat cttccggcgt agtgatgatg agaatttcgc ggataccggc cagcatcagc
    12421 accgacagcg gatagtaaat cattggctta tcgtaaatcg gcaacagttg cttagatacg
    12481 ccgcgcgtaa tcggatgcaa tcgggtgccg gaaccgcccg ccaggataat acctttcatg
    12541 tttgcctccg ctgaaaatta gcctttcaga cctaaacgct cgccctgata gctgccgtcc
    12601 tgcacctgct tccaccagct ttcattagcc agataccact gcaccgtttt acgcattcca
    12661 ctttcaaagg tttcctgcgg cagccagcca agttcacggg caattttcga agcatcaatg
    12721 gcataacgca gatcatgccc cggacggtca gcgacaaagg tgatcaggtc acgatagtgc
    12781 gccacgccgt gcggcttatt cggagccagt tcttccagca gctcgcaaat ggtttccaca
    12841 acatcgagat tcttacgctc gttgtgacca ccaatattat aggtttcacc gactttcccg
    12901 gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcaa catacagcca gtcacggatt
    12961 tgctgcccgt tgccatatac cggcagcggt ttacccgcca gcgcgttgag gatcatcagc
    13021 gggatcagtt tttccggaaa gtggtaagga ccgtagttat tcgagcagtt ggtgataagc
    13081 gtcggcagac cataggtccg cagccaggcg cgcaccagat ggtcgctgct ggcttttgac
    13141 gcggaataag ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag
    13201 tgcaggtcac catatacttc gtcggtggag atatgatgaa aacggaacgc tgatttttta
    13261 tcttccgtca gcgcagtcca gtaagcccgc gccgcttcaa gcagtgtata agtcccgaca
    13321 atgttggttt caataaacgc tgccgggccg tcaatagaac ggtcaacatg gctttcggct
    13381 gccagatgca tgacacagtc tggctgatac tcagtgaata cgcgcgccag ttctgcccga
    13441 tcgcagatat caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac
    13501 atcaggtttc cggcgtaggt cagcgtatcg accaccacca ccgcgtcgct cgtttcgttg
    13561 atgatataac gcaccagcgc cgagccaata aacccggcac cacctgttat cagaatcttt
    13621 ctcatcagcg ccagactcct ttggcatcga cgacatactg ctgatggacg ttgtcgccat
    13681 tgataacttt gaactgacta tgatcgacca gcatcaccag cacatctgcc gttgccagcg
    13741 cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt cttcggcaac tggtggatgt
    13801 taggctcaac aaccagcgtt tcgccgctgt gccactgggc gatcagttca gcgatttcca
    13861 ttgccggact ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca aagcaggcga
    13921 ttttcagttc attggcgcgt ttatcggtag cagccagaca atcagccacc gccgctttca
    13981 cctgatcgat aacccagaac ggtttgtgat cgttcacttc gcgcgcggta cggataagcc
    14041 gcgcctgctg ggggttctgt gccacgataa accacggatc aacagcgatg cagtgaccgc
    14101 ccacgccagg gcctggctga agaatattga cgcgagggtg acgattcgcc aggcgaatca
    14161 gttcccagac gttaatcccc tgatcggcac aaatcagcga caattcatta gcaaaagcga
    14221 tattcacatc gcggaagctg ttttcggtga gcttacacat ttccgccgtc cgcgagttag
    14281 tgacgacaca ctcaccttcg aggaaaattt tgtacagttc gctggcgcgg gccgaacaaa
    14341 ccggcgtcat accaccaatc acgcgatcgt ttttaatcag ctcgaccata acctggcccg
    14401 gtaatacgcg ttccgggcag taagcaatgt tgacgtccgc caactcgccc acctgctgtg
    14461 ggaaagtgag atctggacgc atctctgcta accattctgc catcttctcg gttgacccca
    14521 ccggcgaggt ggattcaagg atcactaacg cgcctttttt cagcactggc gcaatggagc
    14581 gagcagccga ttcaacgtag gtcatatctg gctcatgatc gcctttaaac ggtgtgggta
    14641 cagcaatcaa ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt
    14701 ctacggcagt ttttactacg ctcgccaaat caggctcgac gatatggatt tcgccacgat
    14761 tgatggtatc aaccgcgtgt tggttgatat cgacaccaat cacctgtttt tgccttgagg
    14821 caaacgcagc tgctgtaggc agcccgatgt aacccagtcc gataacagaa atggtcgcaa
    14881 aactcatagt gatatccgat tattttttaa cgcttccaga atgcgagagc atgcctgacc
    14941 atcaccatac gggttatggg cgcggctcat agcttgatat tcgttttcgt cttttaaaag
    15001 acgcgtcact tcctcgacaa ttcgctgctt atccgtgccg accagacgca ccgtacccgc
    15061 cgtcaccgct tccggacgct cagtggtatc gcgcatcacc agcaccggtt tccccagcga
    15121 aggcgcttct tcctgaatgc cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag
    15181 ccagacaaac ggtaaatact cctggggatc gatcagaatg acatttttca catgccccag
    15241 aatgcgattg accggctctc tgacgttcgg gttgagatgc accggataga caatctggat
    15301 gtcctggtgc gtggtggcga tgtctgccag cgcatggcag atttcttcaa agccacgacc
    15361 gaagctctca cgcctgtgac cggtcaccag aatcatcttt ttatcggggt cgataaacgg
    15421 gtaatttgcc gccagttctg aacgcagcgt atcgctgctc atcacctggt cacgcaccca
    15481 taacagtgca tcaatgactg tattaccagt aatgaagatt cggctatccg caacgttttc
    15541 acgcagcaag ttttgccggg aagtttcggt tggagaaaaa tgatacatcg ccagatgccc
    15601 ggtcaatgta cggttagcct cttccggcca cggcgaatag agatcgcccg tgcgcagacc
    15661 agcctcaacg tgaccaacag gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt
    15721 cgtcgtagta tcgccgtgaa ccagcacgac atctggtttg aactcggcaa gaataggttt
    15781 tagcccttcc agaatccgac aggttatctc tgtaaggcct tgtcctggct gcattatgtt
    15841 gagatcgtag tcaggtacaa tggaaaagag tttcagcacc tgatcgagca tctcccgatg
    15901 ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct tttgccaacg catgcaccaa
    15961 cggtgccatc ttgatggctt ccgggcgcgt accaaataca gtcagtactt tcacatcgat
    16021 tctcttcgaa taagcggcga gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc
    16081 aacggcgggt taatgcgaca ccagccccga tcagtccccc gacaatgccc cacataatca
    16141 tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc
    16201 gataggtctg aaaacgcgga tccagggttg gaccaacatt cagggtgttt aacatggccc
    16261 gattttgatc atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag
    16321 cctggagcat tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca
    16381 catctgtcgc actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgttcaatac
    16441 tgttcatccg gcggtcatag atggctttcg ccacctcttc ctgacgcttc acctgagctt
    16501 tcatctggat agtgcgtgcc gcccatgcgc ctttcagctc atcattcaga tggctggctg
    16561 cacgctggct ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt
    16621 cggcaataag cttcacgctg tcattgaccg cgcgggtaaa gtcaccggga ataaacacga
    16681 tgttgttaat catttcatcc agcaacgccg catcggcttt gctgttgccc accatccgct
    16741 gtttgtaata gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca
    16801 taacaaactc tttataagct tcgtccatga ccgatggttg gtcggcagaa gccatgtttg
    16861 aacggacatc caggttacgc aaaaattgct gctgtgagta gtacccgccc agcatattca
    16921 ccgttggacg atcggtaatc gccgtcgcgc tccactcctg acgagcaaaa aaagtatacg
    16981 ccagcgcgat taacgcaaac gccagcccca tgccaataat ccatagcttc ccagcccaca
    17041 aggtacgaaa caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag
    17101 gcattggttg tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt
    17161 tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttgat
    17221 gcaatatcca tagaggaaga atgctagcaa aaagagcacc agcatgaccc actccgggac
    17281 aaaatgagaa tattctgcca gcacgccaat ggaagcgagc aatgctgcgg caagggtaat
    17341 cagcacaaac gcctggcggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg
    17401 acggtctgga gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat
    17461 atccattagc ggaatggcga taatccacaa agcggtaacc gggctgatgg gatgagtttt
    17521 gccctgggtc gtttcgagca ggatccagat aacggtaaaa ccaatcagcg tactgcccgc
    17581 atcccccata aagactttgt agcggcgacc caggatacca aggttaagca tgatgtatgg
    17641 caggatggcg gcgatcatcg caaagcacca gattgcgaga ctggtttgcc cgtcgaacca
    17701 caaaatcata ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat
    17761 gccatcaacc atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta
    17821 accaaacggt ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata
    17881 aagtttgccg aacaccatca taacaatgcc aacagcggcc tgtatggtgg ctcggatttt
    17941 tacgctgata tcaaaacggt catccagcgc gccaatgaaa acaagcacac cagcacaagc
    18001 gagatagaga gatgcatgcg gaatatagta gtcgacaatt ccgaacgtga agcaaatccc
    18061 tgcgtaaacc gaaatccccc caacgagagg tatcaatccc tggtgacgtt tgcggaagtt
    18121 tggtttatcc actaaaccga ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt
    18181 cgtgaataaa aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc
    18241 tctcagagaa aattattagc agaagtataa ccacgaagac ctttattcag aaaggaaagt
    18301 ccgttccgaa cctaccagca ctatgcgaaa attaaccact tgatatgcaa attgcataac
    18361 acaccagcaa attccaggaa ttttcctaac atcgccagcg cggcattaag attacagata
    18421 taaaagcaaa acgccacgta aacacgtggc gtttttggca taagacaaat ttatgagcgt
    18481 ttcatcattt cgaagaaatc gtcattggtc ttggtcattg ccagtttatt aatgaggaat
    18541 tccattgcat cgatttcgcc catcgggtga atgattttgc gcaggatcca cattttctgc
    18601 agttcttcct gagtcgtgag cagctcttct ttacgggtac cggaacggtt gtagtcgata
    18661 gccgggaaga cgcgtttttc agcgatctta cgagagaggt gcagttccat gttgcctgta
    18721 cctttaaact cttcgtagat aacttcgtcc attttagaac cggtatcgat aagcgccgtc
    18781 gcgataatgg tcaggctgcc gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc
    18841 ggacgatgca gggcgttggc gtccacacca ccggtcaaca ctttacctga cgccggaaca
    18901 acggtgttgt aagcgcgcgc cagacgagtg atggagtcga gcagaatgat aacgtctttc
    18961 ttgtgctcaa ccaggcgttt cgccttctcg atcaccattt ccgcaacctg aacgtggcga
    19021 gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt ttaccagtcg ctgcatctcg
    19081 gttacttctt ccggacgttc gtcgatcagc agaaccatca gcacacaatc cgggtggttg
    19141 taagcaatgc tctgagcaat gttctgcagc agcatggttt taccggcttt cggcggtgcc
    19201 acaatcagac cacgctgacc acgaccgata ggtgatgcca gatccagtac gcgagcggtt
    19261 aaatcttccg tagaaccgtt accacgttcc atacgcagac gagagtttgc gtgcagcggg
    19321 gttaagttct caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg
    19381 ttaactttca gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat cttaccagag
    19441 atggtatcac cagtgcggag gttgaaacgg cggatttggc taggggaaac gtagatgtca
    19501 tcaggaccgg cgaggtagga gctgtctgcg gaacggagga aaccaaatcc atcctgcaat
    19561 atctccagta cgccatcacc aaagatatct tcgccactct ttgcgtgctg cttcaggatg
    19621 gcaaaaataa tgtcctgctt acgcatacga gccaggtttt ccagccccat attttcgccg
    19681 agagtgatca gctcagaaac cggcgtattc tttaattcgg taagattcat agtggtgtga
    19741 gttcttaaac ttggggtaaa tctcgaactt aatgttgtga atggtatggc aggatcatcc
    19801 atgcctgttt acggtcatcg actcatgtct tttcgctgcc tggtcacagg agagtacgca
    19861 gaactgaaac gacaagacgg attgagtgac gagccagaaa tctgttcact tcgcatttaa
    19921 gataaaacgg gaagcgttgg aaattacaag attcaaactt aataaggtat gtttaatacg
    19981 aagtcaacac taagttagca tgactcacgc cgggcgtcca gtttttagcg acggggcacc
    20041 cgaacatgaa attcccttac gccaggttag cgtcgaggaa ctctttcaac tgacctttag
    20101 acagtgcgcc cactttggtt gccgccactt caccgttttt gaacagcagc agagtcggga
    20161 taccacggat gccatatttc ggcgcagtgc cagggttttg atcgatgttc agttttgcaa
    20221 cggtcagttt gccctgatat tcgtcagcga tttcatccag aatcggggcg atcattttgc
    20281 acggaccgca ccactctgcc cagaaatcga cgaggatcgc cccgtccgct ttgagtacat
    20341 ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc gctcatatat aactccacag
    20401 gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta aaggttgact
    20461 ttatttcacc ggatacgctt tcgtaaagca atagtaagct gatattctac cacactatga
    20521 gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc cctgcatccg aaggttgtag
    20581 aagcccttga aaaaaaaggg tttcataact gtacgcccat tcaggcactg gcccttccgc
    20641 tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg
    20701 cgtttcttac gtcaacgttt cattatcttc tctctcatcc cgcgattgct gatcgcaagg
    20761 tgaatcagcc gcgtgcctta attatggcac cgacgcgtga acttgccgtg cagatccacg
    20821 ccgacgcgga accgctggcg gaagctaccg gcctgaagct gggtctggct tacggtggtg
    20881 atggctacga caaacagctg aaagtgctgg aaagcggcgt tgacattctg attggcacca
    20941 cgggtcgttt aattgactac gccaagcaga accacattaa cctcggtgcc attcaggtgg
    21001 tggtgctgga cgaagccgat cgcatgtacg atctgggctt tattaaagat atccgctggc
    21061 tgttccgccg tatgccgcct gcaaaccagc gcctcaacat gctgttctcc gccacgcttt
    21121 cgtaccgggt acgtgaactg gcgttcgagc agatgaacaa tgccgaatat attgaagtgg
    21181 aaccggaaca gaaaacgggc caccgtatta aagaagagct tttctaccct tctaacgaag
    21241 aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg gccagacaga gcgattattt
    21301 tcgccaacac caaacaccgt tgtgaagaga tctggggcca cctggcggca gatggtcatc
    21361 gtgtcggttt attgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat
    21421 ttacccgtgg cgatctggat attctggttg ccaccgacgt tgccgcgcgt ggtttgcata
    21481 ttcctgctgt gacacacgtc tttaactacg atttacccga tgactgtgaa gattacgttc
    21541 accgtattgg tcgtacaggt cgcgctggcg caagcggtca ttccatcagc ctggcgtgtg
    21601 aagagtatgc attgaatttg cctgctattg agacctatat tggtcactca attccggtaa
    21661 gtaaatacaa tccggacgca ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc
    21721 cgcgcacagg caatggtccg cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt
    21781 aaagaaaaaa tgtcatactc caaatcattc aagctgtatc aagatggcaa gagaatgaat
    21841 cccgatgagt ttactgtcgt aagtgattca ggtgaaagaa tgcagccaac acagagacag
    21901 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    21961 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    22021 gaataaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctgtctaatg
    22081 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    22141 ctcccccgca aattcgcgtt gtcgctacgg cgacattacg ccttgccgtc aatgcgggtg
    22201 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    22261 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggcggtgcc gatcagcgcc
    22321 tggtggtgga tatcggcgga gccagtactg aactggtaac cggcacgggt gcacaaacca
    22381 cctctttgtt cagcctgtcg atgggctgcg ttacctggct ggaacgctat tttgccgatc
    22441 gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    22501 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggcgct tccggcaccg
    22561 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    22621 agttgcagca actgaaacaa cgcgccattc attgcggtcg gctggaagag ctagagattg
    22681 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatagcca
    22741 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    22801 tggtctacgg tatgttgcat cttaccgtcg agcaggatat tcgcagccgt acgctgcgta
    22861 atattcagcg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    22921 ctaacttctt cgaacaggtg gaaaatgaat ggcatcttga agcaataagc cgcgatttgc
    22981 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagcgccgc
    23041 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaaa
    23101 aactgctggc gacgctactg ctcaaccaga ctaatccggt cgatctctca tcgctgcatc
    23161 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    23221 tcatttttgc caaccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    23281 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    23341 agattattga tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    23401 aatgagtaag tgccggatgc gatgctgaca cattttttcc ggccttgatt atttccctcg
    23461 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    23521 ttcggcgctg accactttgc gctcctgttc ccaaatcaaa tcatcctgcg gcagctccag
    23581 caggaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    23641 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    23701 atcaatattg tcttcatcga tgctgctctg gtggggcaaa aacccttctt ccataccaac
    23761 catgtagaca tagggaaact ccagcccttt cgacgcgtgc agagtcatca gttgcacctg
    23821 atccagttct tcctcgctct cgccgcgctc catcatgtcg cgcagagtaa agcgcgtcac
    23881 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    23941 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgggc tgggcgatgt
    24001 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    24061 ctcccgctcc gccagacgct ggatttctgc cagccagtga gtgaagcggg tcaatgcttc
    24121 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    24181 tttattgcgc gtcatcgccc attcacctag ctttttcagc gtcgccgggc caatctctcg
    24241 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    24301 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac cgccagatat
    24361 tttgtacggg atgcggtttt gcatcaggaa tttttcaaat acccgcgact gatggttgcc
    24421 gcgataaaga atggcgtaat ctttgtactg cgttttattg acgaagtgat gggcgatcag
    24481 ctcgccggtg acgcgctcgg cttcatgttc ctcgttattc gcgcttaaaa cttttagctc
    24541 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    24601 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    24661 caccttcagc gccggaaaat cctggctcag cagcaccaga ttctgcggac gtgcgccgcg
    24721 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    24781 cagcagtttc accagttcat actggctggt gttggtgtcc tgatactcat ccaccagcag
    24841 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gcagcaacgt
    24901 cggcaataaa atcagatcat cgaagtcgag aacgttacag gctttcaggt gtgcatcata
    24961 cagcccataa caatgggcaa aaatacggtc ccgctcgccc tttgcctctg ccgccgcctg
    25021 cgccggtgtt ttgagatcgt tcttccagtt ggagatggtc gaaattagct gttgcaggag
    25081 aactttgtca tcttcaatca gcccctcggt caactcttta agcaaagcaa gctgatcggt
    25141 atcgtcaaac agcgaaaagt tagccttcat ccccagcgcc gcatactcgc gtttgatgat
    25201 atccagcccc agcgtgtgga aggtggaaat catcagccca cgcgcctctt tgcgccccag
    25261 cgtctgcccg acacgctctt tcatctcgcg tgccgcctta ttggtaaagg tcacagccgc
    25321 gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgataac
    25381 acgagtttta ccggaacccg cgcccgccag caccaggcag gggccggtaa cgaattcgac
    25441 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    25501 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    25561 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    25621 tcgagcagat caagaatggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    25681 cgtcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    25741 tcgataaagt ggtgttttct tgcccggtgc tggaaccaac cggaccgctg catacccagt
    25801 tcggttacca catcattaag gtgctatacc gtaattaaca gcaaggcctt ctccgggaga
    25861 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tgcgtttcat atctgtcata
    25921 tagccgcgca gtttcttacc tacctgctca atcgcatggc tgcgaatcgc ttcatttaca
    25981 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccag gtcgcccggt
    26041 tgcagctctg ccataaacgg tttcagcagt ggcacacaag cgtaagagaa cagatagtta
    26101 ccgtactcgg cagtatcgga gataaccacg ttcatttcgt acagacgctt acgggcgatg
    26161 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    26221 ccagaatcaa ccatggtttc aaacgccagt tcaacgcctg cttttaccat cgcgatcatc
    26281 agtacgcctt tatcgaagta ctcctgttcg ccgattttgc cttcatactg cggcgcggtt
    26341 tcgaatgcgg ttttgccagt ctcttcacgc caggtcagca gtttcttatc gtcgttggcc
    26401 cagtcagcca tcatgccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    26461 aacagcggcg ccatgatctc tttcagttgc tcagagagcg cgtaagcacg cagtttcgcc
    26521 gggttagaaa gacggtccat catcagggtg atgccgccct gtttcagcgc ttcggtgatg
    26581 gtttcccaac cgaactgaat cagtttttct gcgtatgccg gatcggtgcc ttcttccacc
    26641 agcttgtcga agcacagcag agagccagcc tgcaacatac cgcacaggat ggtttgctcg
    26701 cccatcaggt cagatttcac ttccgcaacg aaagaggatt ccagaacgcc cgcacggtga
    26761 ccgccggttg cagccgccca ggctttagcg atcgccatgc cttcgccttt cggatcgttt
    26821 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgtact
    26881 tcggtgccag ggcatttcgg cgcaaccatt acgacggtga tgtctttacg gatctgctca
    26941 cccacttcta cgatattgaa accatgagag tagcccagcg ccgcgccgtc tttcatcagc
    27001 ggctgaacgg aacgcactac gtcagagtgc tgcttgtctg gcgtcaggtt aaccaccaga
    27061 tccgcctgcg ggatcagttc ttcgtaagtg ccaactttga agccgttttc ggttgcttta
    27121 cgccaggatg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    27181 ccagaatcac gcatgttcag gccctggttc agaccctgtg caccacagcc gacgatgact
    27241 acttttttac cctgaagata gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    27301 catttgccca gctgtgccag ctgctggcgc agattcagtg tattgaagta gttagccatg
    27361 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    27421 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    27481 tgcaacgtca acattaaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    27541 tctggcggaa agccgccatt ttggccgcag cgcgcgggca atgcacgtta gcccgtccac
    27601 gctctctcgg cagattcagc gcctggaaga cgatctcggt cagccgctgt ttgtgcgcga
    27661 taaccgcacg gtaacgctaa ccgaagcagg cgaagaactg cgcgttttcg cccagcaaac
    27721 gctgttgcag tatcaacagc tgcgccacac catcgatcag caaggaccgt cgctctctgg
    27781 cgaattacat atcttctgct cggtgaccgc tgcctacagc catctgccgc cgattctcga
    27841 tcgcttccgc gcggaacacc cgtcggtgga gattaaactc acgactggtg atgcggcaga
    27901 tgcgatggaa aaggtcgtca ctggcgaagc ggatctggcg attgccggga aaccggagac
    27961 tctgcccggc gcagtggcgt tttcgatgct ggagaatctg gcagtagtgc tgattgcccc
    28021 cgcgctaccc tgcccggtgc gtaaccaagt ttcggcagat aagccggact ggtctacagt
    28081 gccgtttatc atggccgatc agggaccagt acgccggcgc attgagttgt ggtttcgacg
    28141 caacaaaatc agcaacccga tgatttacgc cacggtcggc gggcatgaag cgatggtgtc
    28201 gatggtggcg ctcggctgtg gcgtagcctt gttgccggaa gtggtgctgg aaaacagccc
    28261 ggaaccggtg cgtaaccgcg tgatgatttt agagcgcagt gatgagaaaa cgccgtttga
    28321 actgggcgtg tgtgtgcaga aaaagcggct acatgagccg ctgattgagg cattctggaa
    28381 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    28441 gtggcaatcg taacccaact cattcaaccg ggtttcgaaa tccggttcat ggtcgccaag
    28501 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    28561 aatgttccag tgcgtaccca gcgtgttgag gaagcgcaac agcgcgcccg gcgattccgg
    28621 gaattcgaag ctgtagaggc gttcctgcaa cggatgcgac ggacgcccgc cgaccatata
    28681 gcgcacatgc agcttcgcca tttcgtcgtc ggagagatcc actacgctgt agccgccatc
    28741 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggcca cggctcaggc gcacaccgac
    28801 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgagcgccc
    28861 gccaagcagc tggcagaact tcaggaagct gcccttctct tccgggatgg ttaccgccag
    28921 caacgcttca cgctgttcgc ccagttcgca acgttccgag acgtagcgca ggccgtgaaa
    28981 gttcacgtta gcaccagaaa gaatatgcgc cagccgctca ccgcgaatgt tgtgctgggc
    29041 gatatatttt ttcattcccg ccagtgccag cgcgccagag ggttccgcta ccgcgcgcac
    29101 atcttcgaac aggtctttca tcgccgcaca tatggcatcg ctatcgacgg tgatgatgtc
    29161 gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttca ccgcaacacc
    29221 ttcggcaaac agccccacgc gtggcagatc aaccggatga cctgcatcca gcgccgcttt
    29281 caggcaggcg gagtcttccg cttccacggc aatcactttg atttgcggca tcagttgttt
    29341 gatcagtacc gccacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    29401 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg cgatcaccat
    29461 cggatggtcg aacggcggca cccaggtgaa gccctgctgc tgtgacagtt cgatcgcttt
    29521 cgcttttgct tcatcaaagt tcgcgccgtg aagaagcact tcgccgccga agccgcgcac
    29581 cgcgtcgact ttgatatcgg cggtggcggt tggcatgacg atcagtgcct tcacgcctaa
    29641 ccgtgcggaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcgg tgatcacgcc
    29701 gtgcgctttc tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    29761 gctatgaact ggctgacggt cttcgcgttt caccagaatc acgttatcga gacgcgacga
    29821 gagtttttcc attttttgta gcggcgtgac ctgcgccgct tcgtagaccg gcgcgcgcag
    29881 caccgctctt aaatattcgg cgccttccgg ggcgccggag agaggttgtg agtctgccat
    29941 cattaacccc ccagtttcga tttatcacgc accgcgcctt tgtcggcgct ggttgccagg
    30001 ctggcgtagg cacgcagcgc aaaggaaacc tgacgttcac gatttttcgg tgtccaggct
    30061 ttgttgccac gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    30121 aactgaatgc cacggttcgg aatgtcgata gcgatcaagt caccatcttc aatcaggcca
    30181 atgctgccgc cacttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    30241 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    30301 aaactggttg ggtagagcat ttcctgcatc cccggaccgc ctttcgggcc ttcatagcga
    30361 attactacca catctccggc gacaacttta ccgccgagaa tcgcttctac cgcgtcgtcc
    30421 tggctttcgt agactttcgc cgggccagtg aatttgagga tgctgtcatc gacgcctgcg
    30481 gttttaacga tgcagccgtt ttctgcgaaa ttaccgtaga gtaccgccag gccgccgtct
    30541 ttgctgtaag cgtgttccag cgagcggata cagccattgg agcgatcgtc gtccagcgta
    30601 tcccagcggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgccgg gccagcgcgg
    30661 aacatatttt ttaccgcatc atcctgggtc acaatgatgt cgtattgttc cagcgtttgc
    30721 ggcaacgtca ggccaagtac atttttcaca tcacggttca gtaaccccgc acgatccagc
    30781 tcgccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    30841 gtgctcggcg cgactttaca cagctgcggt actttgcggg agagcttatc gatatcactc
    30901 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagaac ggtgttggta
    30961 gagccgccca tcgcgatatc cagcgtcatg gcgttttcaa acgccgcctt actggcgatg
    31021 ttacgcggca gtgcgctttc atcatcctgt tcgtagtaac gtttggtcaa ttcaacaatg
    31081 cgtttaccag cattgaggaa cagttgcttg cgatcagcgt gggttgccag cagcgagccg
    31141 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcagtaaac
    31201 atcccggagc aggaaccgca ggttgggcag gcggatcgtt caacctgatc gctctgggag
    31261 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag cttaatgatc
    31321 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    31381 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    31441 cagaccatgg cgtcggcgca gtgggcgttg accatatatt caacggaatc agcgatcagt
    31501 tcgcgagatg gcagtgaata aagcatgccc ccgtggccca tagcgatccc atcatccacc
    31561 gcaatggtgt tgaactcttt ggcaacgccg cccgccgctt caatttgttc ggcgaccagt
    31621 ttaccgagat cgcgcagatg aacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    31681 gcgataatcg gcttaccgaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    31741 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    31801 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    31861 ttgattaact tgatctaacc agccccattt atcttcggtc tcgccagtga agaggccgaa
    31921 gaaggcttgc tgaatgcgtt tggtaaccgg gccgcaacgg ccttcaccaa cctgaatacc
    31981 atctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    32041 caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    32101 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggagt
    32161 aaacagcaca ccgtctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    32221 attcacatcc aacgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccaa
    32281 cagggaagag aggtagttac caccagcttt cgctgcggtt gggatggtgt ttggtgcagc
    32341 acggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    32401 cgccccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    32461 gcccatgcca acatcaccga cgaaaatcag cggacggata taggcgctgg tgagattgtt
    32521 tttgcggatc acgtcgcgac aagcttccat cagctcatca atgctctgcg aaaccgggaa
    32581 acgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    32641 cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    32701 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    32761 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    32821 gttgtgatgt ggttgtgctc tggcagatag caacgtgtgc gacgtccacc agtttattta
    32881 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    32941 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    33001 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    33061 gttgcatcat gataatttct ccaacatttc agaattactg gcaccaggcg gcaccagcgg
    33121 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    33181 catggtgtcg agtgccgctt caacctggtc tttatgggtg atgtgttggc cagggatgcc
    33241 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    33301 ttcctggaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    33361 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    33421 aaaagagccg tcaccggaga tacagacgac agtatcgttc ggtcgcgcga cttgtgcgcc
    33481 aaccgccgcc ggtaaaccga aacccatggt gcctaagccg ctggaggtga tgaagttttc
    33541 cgggcgagtg tggacgatgt gctgggcagc ccacatctgg tgctgcccca catctgtggt
    33601 cacgatgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gcgcgtagat
    33661 agcgtcacca ggatggtcgt aacgccaggc atgttcatca cgcagttgcg cgcagtgttg
    33721 ctgccagtca tcgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    33781 ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccggatcga tatccatatg
    33841 gataacgctg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    33901 tgcgcccacg gctatcagta gatcgcactc ctgcactgcg aagttcgccg ctttggtgcc
    33961 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    34021 caacgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccgaaactgc
    34081 ctgcgccatg cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    34141 ctgacgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cagtggtgaa
    34201 ccacggttcc agatcgccgc tggctaactg gatatctttt ggaatatcga ccaaaaccgg
    34261 accaggacga ccagagctgg cgacgtcaaa cgcttcggcc atgatacgcg gtaactcttc
    34321 cagtgattgc accaggaaac tgtgcttggt gcaggctaac gacaacccca gaatatccac
    34381 ctcctgaaat gcgtcagtgc cgataaacgg tgcggatacc tgaccggtga tagcaacaac
    34441 ggggatggaa tctaacagcg cgtccgcaag cccggttatc aggttggttg cacctgggcc
    34501 agacgtggcg atgcaaacgc cagttttgcc ggtagcacgg gcataaccga tagccgccat
    34561 tgctgcacct tgctcgtgtc ggcacagcaa gtgttccacg ccaccgtcat acaatgcatc
    34621 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgtttacac cctgtgcccg
    34681 caacgcatgt accacccact gtgcaccatt catagttagt tccccgtcct gaatcttgag
    34741 aaacagaatt ttgtgctgtt attcattgtc tgctcctcag ttatgttttt aaggtcaaaa
    34801 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    34861 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    34921 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    34981 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttt ggccgatttt
    35041 tatgatgtct ttaataatct caagagaaat tgttgttttt taaaaagaaa atagtgagtg
    35101 aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc ctttgcccgc
    35161 tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa
    35221 caggaatcag ccactaaaat tcacgaattt gcgggctaca gcagcgatat cttatgcaca
    35281 taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc gcagccctgg
    35341 gagtaaatgc gcccccaatc actgttgagg tacatatcag taaaggtcta ccaggcttaa
    35401 cgatggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg cgcagcgcca
    35461 ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt gctccagccg
    35521 atctgccaaa agaaggggga cgatatgatt tgcctatcgc tattgcgttg ctggcggcct
    35581 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctggcgctta
    35641 caggcgctct gcgtggcgtt cccggcgcaa tctccagtgc aactgaagct attaagtcgg
    35701 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    35761 gatgcctgat agccgatcat ctgcaggctg tctgcgcgtt tctggaaggt aagcacgctc
    35821 tcgaacaccc gaaaccaact aatgcagtat cccgggcgct acaacatgat ctcagtgatg
    35881 ttgtcggtca ggagcaagga aagcgaggac tggaaattac cgccgctggc gggcacaacc
    35941 ttttactgat tggaccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc
    36001 ttttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa
    36061 atgctgaatc agtacaaaaa caatggcggc aacgcccgtt ccgctcacct catcacagtg
    36121 cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgctgg
    36181 cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg
    36241 ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa
    36301 cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc ggacattatc
    36361 agggaaacca taaccgctgc acaccagaac agacattacg ttatctcaac cggctctcgg
    36421 ggccctttct cgaccgcttc gatctctcac tggagatccc attaccgcct cccggcattt
    36481 tgagtaaaac ggtagtgccg ggagaaaaca gcaccaccgt taaacaacgt gtaatggccg
    36541 ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat aatccggaaa
    36601 tacgccaatt ctgcaagctt gagagcgaag atgcgcagtg gctggaagaa acgctgatcc
    36661 atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga accattgctg
    36721 atattgatca gtctgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgtg
    36781 cgattgaccg tttgctcatc catctgcaaa aactactgac ataaaaaaag ggcatttcgc
    36841 cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg cggtttaccg
    36901 ccggaaaggg tgtgaaaacg ttttggacgc ttgatacgcg tcatatactt ggaccacacg
    36961 cgttctgctt ctgtcactgg ttcacgttcg ccacggcata ctgctacgaa gagtttctct
    37021 tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata accatgacgc
    37081 tcaagcagtt gtgcctcttt gatggtgaag tcaccatgac gagagaatcc acgtggataa
    37141 tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct gcacgctcct
    37201 aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa
    37261 caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga tacggaattg
    37321 ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc
    37381 tatctgaccc aatcagccgt gagctttcga atcagacaac tggaaaatca actgggtgtg
    37441 aatcttttca cccgccacag aaacaatatc cgtttaaccg ctgccggaga aaagcttctg
    37501 ccttatgcag aaacgctcat gagcacctgg caggccgccc gcaaggaggt ggcgcatacc
    37561 tcacgacata atgagttttc tatcggtgcc agcgcctcgc tgtgggagtg tatgcttaat
    37621 cagtggctgg gacgcttgta tcaaaatcag gatgtccata caggcttaca gttcgaagcg
    37681 cgaattgccc aacggcagtc tctggtaaaa cagttgcatg aacgccagct tgatcttctt
    37741 attacaactg aagcccccaa aatggacgaa ttttgtagtc agttgctggg gtatttcact
    37801 ttagcgcttt ataccagtgc cccttctaaa ctaaagggag atcttaacta tctgcgacta
    37861 gagtgggggc cagactttca acagcatgag gcaggtttga tcggtgctga cgaagttccc
    37921 attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct taatggttgc
    37981 acctggctac ccgtcagttg ggcacgtaaa aaaggcggcc tgcataccgt tgtcgatagc
    38041 acaacacttt cacggccgct ttatgccata tggctacaaa atagcgataa aaatgcgttg
    38101 attcgcgatc tattgaaaat taacgtgctg gatgaagtgt attaatctga atggctggca
    38161 aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa aaatccttag
    38221 ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt
    38281 tggagaccgg tgctctacca attgaactac gcccctaatt agggtggcgg aacggacggg
    38341 actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc
    38401 caccgaattc ttttacaacc accggtttta tgaccggctt actgcttaat ttgatgcctg
    38461 gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac
    38521 ttctgagttc ggcatggggt caggtgggac caccgcgcta aggccgccag gcaaattctg
    38581 ttttatcaac acgtcccgtt atgacctgtt gattcaatct g
//
LOCUS       DAJOTB010000026        38619 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJOTB010000026 DAJOTB010000000
VERSION     DAJOTB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.026"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(124..1788)
                     /gene="glnS"
                     /locus_tag="OHN28_001385"
     CDS             complement(124..1788)
                     /gene="glnS"
                     /locus_tag="OHN28_001385"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCQ2296811.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2008..2769)
                     /locus_tag="OHN28_001386"
     CDS             complement(2008..2769)
                     /locus_tag="OHN28_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2296812.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2771..3757)
                     /locus_tag="OHN28_001387"
     CDS             complement(2771..3757)
                     /locus_tag="OHN28_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HCQ2296813.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3750..4985)
                     /locus_tag="OHN28_001388"
     CDS             complement(3750..4985)
                     /locus_tag="OHN28_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCQ2296814.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            5155..6312
                     /locus_tag="OHN28_001389"
     CDS             5155..6312
                     /locus_tag="OHN28_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCQ2296815.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6316..7203
                     /locus_tag="OHN28_001390"
     CDS             6316..7203
                     /locus_tag="OHN28_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCQ2296816.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7263..8021
                     /locus_tag="OHN28_001391"
     CDS             7263..8021
                     /locus_tag="OHN28_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2296817.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            8057..9226
                     /locus_tag="OHN28_001392"
     CDS             8057..9226
                     /locus_tag="OHN28_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HCQ2296818.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9223..9693
                     /locus_tag="OHN28_001393"
     CDS             9223..9693
                     /locus_tag="OHN28_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2296819.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9707..10987
                     /locus_tag="OHN28_001394"
     CDS             9707..10987
                     /locus_tag="OHN28_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCQ2296820.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(11076..13022)
                     /gene="nagE"
                     /locus_tag="OHN28_001395"
     CDS             complement(11076..13022)
                     /gene="nagE"
                     /locus_tag="OHN28_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCQ2296821.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13355..14155
                     /gene="nagB"
                     /locus_tag="OHN28_001396"
     CDS             13355..14155
                     /gene="nagB"
                     /locus_tag="OHN28_001396"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2296822.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            14215..15363
                     /gene="nagA"
                     /locus_tag="OHN28_001397"
     CDS             14215..15363
                     /gene="nagA"
                     /locus_tag="OHN28_001397"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2296823.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15372..16592
                     /gene="nagC"
                     /locus_tag="OHN28_001398"
                     /gene_synonym="nagR"
     CDS             15372..16592
                     /gene="nagC"
                     /locus_tag="OHN28_001398"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCQ2296824.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16640..17392
                     /gene="nagD"
                     /locus_tag="OHN28_001399"
     CDS             16640..17392
                     /gene="nagD"
                     /locus_tag="OHN28_001399"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCQ2296825.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17648..19312
                     /gene="asnB"
                     /locus_tag="OHN28_001400"
     CDS             17648..19312
                     /gene="asnB"
                     /locus_tag="OHN28_001400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCQ2296826.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(19354..19791)
                     /locus_tag="OHN28_001401"
     CDS             complement(19354..19791)
                     /locus_tag="OHN28_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296827.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            20125..20201
                     /locus_tag="OHN28_001402"
     tRNA            20125..20201
                     /locus_tag="OHN28_001402"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20159..20161,aa:Met,seq:cat)
     gene            20212..20296
                     /locus_tag="OHN28_001403"
     tRNA            20212..20296
                     /locus_tag="OHN28_001403"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20246..20248,aa:Leu,seq:tag)
     gene            20320..20394
                     /locus_tag="OHN28_001404"
     tRNA            20320..20394
                     /locus_tag="OHN28_001404"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20352..20354,aa:Gln,seq:ttg)
     gene            20429..20503
                     /locus_tag="OHN28_001405"
     tRNA            20429..20503
                     /locus_tag="OHN28_001405"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20461..20463,aa:Gln,seq:ttg)
     gene            20519..20595
                     /locus_tag="OHN28_001406"
     tRNA            20519..20595
                     /locus_tag="OHN28_001406"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20553..20555,aa:Met,seq:cat)
     gene            20644..20718
                     /locus_tag="OHN28_001407"
     tRNA            20644..20718
                     /locus_tag="OHN28_001407"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20676..20678,aa:Gln,seq:ctg)
     gene            20756..20830
                     /locus_tag="OHN28_001408"
     tRNA            20756..20830
                     /locus_tag="OHN28_001408"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20788..20790,aa:Gln,seq:ctg)
     gene            complement(20985..22160)
                     /gene="ubiF"
                     /locus_tag="OHN28_001409"
     CDS             complement(20985..22160)
                     /gene="ubiF"
                     /locus_tag="OHN28_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCQ2296828.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            22306..23730
                     /gene="miaB"
                     /locus_tag="OHN28_001410"
     CDS             22306..23730
                     /gene="miaB"
                     /locus_tag="OHN28_001410"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCQ2296829.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            23883..24923
                     /locus_tag="OHN28_001411"
     CDS             23883..24923
                     /locus_tag="OHN28_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCQ2296830.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            24920..25387
                     /gene="ybeY"
                     /locus_tag="OHN28_001412"
     CDS             24920..25387
                     /gene="ybeY"
                     /locus_tag="OHN28_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCQ2296831.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            25477..26355
                     /gene="corC"
                     /locus_tag="OHN28_001413"
     CDS             25477..26355
                     /gene="corC"
                     /locus_tag="OHN28_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCQ2296832.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            26380..27918
                     /gene="lnt"
                     /locus_tag="OHN28_001414"
     CDS             26380..27918
                     /gene="lnt"
                     /locus_tag="OHN28_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCQ2296833.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSTAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYSRTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(28045..29922)
                     /locus_tag="OHN28_001415"
     CDS             complement(28045..29922)
                     /locus_tag="OHN28_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCQ2296834.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            30376..31284
                     /gene="gltI"
                     /locus_tag="OHN28_001416"
     CDS             30376..31284
                     /gene="gltI"
                     /locus_tag="OHN28_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCQ2296835.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31454..32194
                     /gene="gltJ"
                     /locus_tag="OHN28_001417"
     CDS             31454..32194
                     /gene="gltJ"
                     /locus_tag="OHN28_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCQ2296836.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32194..32868
                     /gene="gltK"
                     /locus_tag="OHN28_001418"
     CDS             32194..32868
                     /gene="gltK"
                     /locus_tag="OHN28_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCQ2296837.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32868..33593
                     /gene="gltL"
                     /locus_tag="OHN28_001419"
     CDS             32868..33593
                     /gene="gltL"
                     /locus_tag="OHN28_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCQ2296838.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            33711..34646
                     /gene="rihA"
                     /locus_tag="OHN28_001420"
     CDS             33711..34646
                     /gene="rihA"
                     /locus_tag="OHN28_001420"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCQ2296839.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            34730..36400
                     /gene="hscC"
                     /locus_tag="OHN28_001421"
     CDS             34730..36400
                     /gene="hscC"
                     /locus_tag="OHN28_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCQ2296840.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(36460..37911)
                     /gene="djlC"
                     /locus_tag="OHN28_001422"
     CDS             complement(36460..37911)
                     /gene="djlC"
                     /locus_tag="OHN28_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCQ2296841.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37908..38615)
                     /locus_tag="OHN28_001423"
     CDS             complement(37908..38615)
                     /locus_tag="OHN28_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2296842.1"
                     /translation="MNKEEQYLLFALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9396 a        10093 c         9730 g         9400 t
ORIGIN      
        1 aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa aaacgccgca gagcgcggcg
       61 tttgggataa tttgcatatt tcttgccgga tgcggcgtaa acgctctatc cgacaacgta
      121 aacttactcg cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg
      181 tttttccgcc gtagaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa
      241 tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag
      301 cgattccggg ttaatcaccg acaggaaatc atccgcagca ccagggttcg gcacgctgaa
      361 cagacggtca tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat
      421 aacacctttg actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt
      481 acagaagatg gtggtgatat tgccttcggc atctttctcc acgcgttctg ccttaatgac
      541 gtaagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc
      601 ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg ctaaatggca cctgacggct
      661 gcccatttcc ggtttattcg gatggttcgg catggtaacc atttcgcctt cgccctggta
      721 gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt
      781 gagatcttca cggatgcagg attccagtga cgccatctca atggtgttgt cctgcttggt
      841 cacaccaatg cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag
      901 accagaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag
      961 caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta
     1021 ctggcgcggg tgaacaggaa tggtgatgtt gtccagtacc cagtcgtaca gacgacggtt
     1081 gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagcgcat cgctgatgca
     1141 gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc
     1201 aaacttaatg cggtacagca ccggatcgcg catcacgata aacggcgaag ccatgtcgat
     1261 tttcgcacgc aggcaggctt taccttcttc aaaaccgccg gtacgcattt tctcgaacag
     1321 cgccaggttc tcttcaacgc tgcgatcgcg gtacgggctg tttttacccg gctgcgtcaa
     1381 ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca tcaacgtacg ccaggccttt
     1441 attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac
     1501 gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat cgacatactc
     1561 gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc
     1621 ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt
     1681 cggctccggc gggaaacggg tgtgtactgt ggtgtgctta ccactggcca gatcttcatc
     1741 gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc
     1801 aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt
     1861 agttagcgaa aaaatggttt tgcgtaagat attggggcgt tttctgcgct actgccagca
     1921 gatcctcgcg ctggaaacgt aaggccaaac gtcgccagcc tgacgcattt attaaatttc
     1981 cggatgagct ggccgcagag tcgtagatta gtcggctatg attaaacgcg tcgacaagga
     2041 cgcaagcgtt ctacgcgtct gcccatcaag ctgactatcc gtaataatct ctgtcagttc
     2101 attaagatgc gcgatattaa acagcgacca tgaaccgtat tttgagctgt cagccaggag
     2161 aattttgcgc gaagcatgcg cgataagatc gcgttttagt gcggctttct cttccgtggg
     2221 tgctgtaata cccttttcaa gatcccagct gttacagctt aaaaatgcca cgtccggcca
     2281 ggtattttgc agcaatttac gaccatgttc gccaatgcag gactggctgg agtcgtcaat
     2341 acgtccgccg ataatcgtca cttcaatttg cttgaattca gccaaaaaaa gcgcgatatg
     2401 caggtcgctt gtgatcacgc gaagcggtaa atgtgtaatc tgccgcgcaa gttcaatcat
     2461 tgtcgtaccg gcatcaagga cgacggaatc ccccgctttg accattgacg ccgctgcgct
     2521 ggcgatggca tgtttttccg ccaggctgcg ctgcattttc tcatgggtag tcggttgggt
     2581 aggaataaac cggttgagag tcacaccgcc gtgtgtacgg ctgataacac cctgctcatc
     2641 gagttttatc aggtctcgac gaatcgtagc cggcgacgca ttggttaccg ccaccagctc
     2701 atctacggtg accagattat ggcttttcag gtaatccatg atctgctcta accggttata
     2761 tcccttcatt ttattctcgc gaaatctgca ttgctaactg gatggaaact gccatgctct
     2821 cagacttggc ttttcctgtc caggcaatat caaacgcggt gccgtggtcg gcagaagtgc
     2881 gaataaatgg caacccggcg gtgatattca ccccatcata aaatccaagc agttttaaag
     2941 ggatatgccc ctgatcgtgg tacatagcca ctaccatatc gaatagccct tcatggcatt
     3001 gcataaacac ggtatctggc gggcacgggc cagtaacatc aatatcttcg gcctgcattg
     3061 cttcaatcgc aggcgcgata atctcgattt cctcagtgcc gaataaacca tgttcccctg
     3121 catgtggatt tacacccgcc acggcaatac gcggacgctc aataccaaca cgtttcagga
     3181 agtggttcgc aactcgaata accgttttga ccctttcacc attcaaagta tccaggaatt
     3241 tacgcagtga gatgtgagtc gaaatatgga taaccttgag cttctcggta tagagcacca
     3301 tcgcgtactc tttgctgccg gtaagatggg ccagcaattc cgtatgcccc ggatagttat
     3361 gcccgccaag atgcagcgct tctttattca gcggcgcagt ggctatggct ttcacctcgc
     3421 ctgataaggc cagagccgtt gcacgtttga tacagcgata cgctaaatcg cctgccacag
     3481 cctgtaccac tccgggtgtc agcgcatcgg ggttagccag cggctcatcc atcacgttaa
     3541 tcacgccggg agcaaaatgg gcgtcgcaca ccttttgaat gacgcgtaac tctgcctgcg
     3601 gcgtgatatt catcgccata atacgtcgca gcgtgcgcac acagcccacc accaccaccg
     3661 gggcaccagc aagcccggct tcgctcaggg atttaataat gatctccggg ccaatacccg
     3721 ccggatcgcc catcgttacc gcaataatat tattcactac gcttctcctc gataaaattc
     3781 agaacctgat gcaacgtggt ctcatcgcca aacccaccgg ctttcgtcat caccgaacgg
     3841 gaccagacgc cgcccagaaa gtgaccgtat ggaacgcatt gcgccacctt ccccgtaata
     3901 cgaaatccgg tcgcgcctaa cgcactggct gttgccattg cgacatcacc accagagaga
     3961 taaagcgcat cgggcattgc gcaaagcaga acttgccggg taagttcgcc aagaaaccgg
     4021 caaatttttt ccccaagtgc agcgcgactc atctgccaac gctggcaaag ggtgtcgatc
     4081 tgatgccttg ctacagaatc gttacaggtg tggacaatac agtggtcgcc tttctgcaag
     4141 gcctgcacaa tacgcgcgtc atagtcactg gcattgcctg ccagaatgtg ttcaacatcg
     4201 acgtatatct gtgtgatacg tgggtgtgaa tgaagtgtgg cgatctgccg ttgagcgatt
     4261 tcgctcattg aaccaataat ggccagcacg gtgggtggcg gcataatcgc ggagcgccgg
     4321 gcaacggcat cacaaatacc agcggagccg accagcagca gcggttgttt agcctgcatc
     4381 acagcggcag cgatgcgatc caggtcgctg tccgtctgcg catctatcac gccaatccac
     4441 ggttgctcat aagacaaatg gctgagctga gaaaccgtaa cgggctggca tggaatggcc
     4501 gtctgcaacc ggatgatgtc cgcaattcgg gcgctcaata ccggggtttt cggatcgcta
     4561 gcaaactcgg tttccgttat ccgcacaccg tttacccaac actcaccggc cactgtggtc
     4621 cttcccgcct gaggaaatgc gggggcaacc actgcgcccg ttataccgca tgccttcatc
     4681 agggcttcaa tttctgcgcc gatattgcct cgcagcgtgg aatctatctt tttaatcacg
     4741 agcggcgggt tattcgccag agggagatga ctcatcagcg aggttatttt catcgccgcc
     4801 agcttaggat ccatcgcccg actgtcgctg ttaatcaccc atacgctggc atcaccgcga
     4861 taaggcattt caaaggccac atcaactgta tgccccacct gcgccaggct tacccccgcg
     4921 tcgttggctc ccgtgaaatc atcggcaacg atgagaatgc tcggcttagt gattcccttt
     4981 tccatcatca ctccaggttg ttttatgata acaatgatta tattcaatca tgattgatta
     5041 aatgtgagca agctcaagtt attaatcact cgaataaatt ataaaattag accatgttac
     5101 gtaggctacg gcattttgaa tgattgaatt caatcaccag aagagaggtt atttgtggta
     5161 attatcaata gcacgatact gagcggcgca ggcgctatcc cttccctgac gtcgctctta
     5221 cccgacatca gaaaaatgct gctggtcact gaccgtaata ttgcgcagct cgacggtgtg
     5281 cagcagattc gcgccttact ggaaaagcac tgcccgcagg ttaacgttat cgataatgtg
     5341 cccgcagagc ccacgcatca tgatgtgcgc cagctaatgg atgcccttgg cgatgcctct
     5401 tttgatgtgg tggtcgggat cggcggtggc agcgtgttgg atgtggcgaa gctgctatcg
     5461 gtgctttgcc atccacaatc accggggctg gatgcgctgc ttgcgggtga aaaaccgact
     5521 cagcgggtgc aatcatggtt gattcctaca accgccggaa ccggctcaga agccacgccg
     5581 aatgcgattc tggcaatccc tgagcaaagc acgaaggtgg gtattatttc ccaggtgctg
     5641 ttaccagact atgtggcgct tttcccggaa ctgaccacca gtatgcccgc gcatattgcg
     5701 gcgtccacgg gcattgatgc tctttgccac ttactggagt gttttaccgc gaccgtggca
     5761 aatccggtca gcgataacgc ggcgctgact gggttaagta aacttttccg gcacattcaa
     5821 cccgccgtga acgatcctca ggatctgcgc gcaaaactgg aaatgctgtg ggcgtcttac
     5881 tatggcggcg tagcgataac ccatgcgggc acgcatctcg ttcatgcgct ctcctacccg
     5941 ttaggtggca aatatcatct gccgcatggc gtcgcgaatg ccatcttgct ggcgccgtgc
     6001 atggcgtttg ttcgcccctg ggcggtcgag aaatttgccc aggtctggga ttgcattccc
     6061 gatgcggaaa ccgccctgag cgcggaagaa aaatctcatg ccctggtgac ctggttacag
     6121 gcattagtca atcaactcaa gctacccaac aatctcgcgg atctcggcgt accgccagag
     6181 gatattgcct ctctgagcga ggcggcactg aacgtgaagc gccttatgaa caatgtgccg
     6241 tgccagcttg atctacagga cgtacaggcc atttaccaaa cactgtttcc gcaacatcaa
     6301 tttaaggaat aaatcatgcc gaaaaccatt gaaggtgtat taaccgctat cgtcaccccg
     6361 tttaccgcga ccggcagtct gaatatcccg gcgttaaaag tgcaggtgaa tcgtcaactg
     6421 gaagcgggta acgcgatttt ttgcggcggc accaacggcg agttttttgt gttgaatgaa
     6481 caggagaagc tcagcgttac gcaaacctgc gtggatgaag tggctggtcg tgcgccagtc
     6541 gtggcacaca ttggcgagat ctctacgcgc gaaacgattc gtctgggtca gcagattgaa
     6601 aaacttggcg tagatgccgt gtcggtcatt gccccttact tcgtaccgct gaaacaggaa
     6661 gagttgattg cgcattacag cgcaattgcc gacacactga gcattccgat gtttctctat
     6721 aacatcccgg cccgcactgg caatacgatc cagccagaaa ccgcgcgtgc tctggcgtcg
     6781 caccccaata tcattgggat taaagacagc gacggaagcg acgaaagcct gaagggtttt
     6841 ctcgatgcgg tgcgcgacat tgacggcttt aatgtgctca acggcccgga ttcgctgatc
     6901 cataaagggt ttgtcgaggg atgttcagcc tgtatctctg ggctcgccaa cgtcgcgccg
     6961 cgagaaatca atgccatctg gtctcgcttc catgccggtg atattgaagg gtcttatgaa
     7021 gcacaggaga gtgtaacggg cctgcgtacc gatctttata aagtagcatt ctccccggct
     7081 gcggtgaaaa aagcgttgca gcttatgggg catgatgttg gtgatagccg ctacgcggtt
     7141 aatttcacgc ctgaacagat cagcgagatt caacaaatta ttcgtcatta taacattaat
     7201 taaattaact ctattatcca gcccgctata aagtttagat aaagagaata aaggattacc
     7261 ccatgttttt gtggtttatg cagccagata tctggctcac tttaattacc ctcacctttc
     7321 tggaaattat tcttggggtg gataatatta tttttctttc actggtggtg gcaaaacttc
     7381 cgttatcaca acaaaatatg gcgcgtaaac tgggtttaag ctgcgcgatg attatgcgga
     7441 tattattgct gatatccatt gcgtggcttt cacatattac ccagccgctt tttaccgtgc
     7501 tgacgctgga gatttcgttc cgcacgttga ttctgctcgg gggcgggctg ttcctgattt
     7561 ataaagcgat acaggagata cgcgaagagc tgtcgcccgc gcaggaagac gaacatggca
     7621 gcggatcgcg gcaactgtcg ttgtgcggtg ccattgtaca gataatgctg ctcgatatcg
     7681 tgtttagcct ggattcggtg atcaccgccg tcggcctttc tcagcatata tttatcatga
     7741 ttgccgccgt aatgattgcc gtcggtgtca tgatgtttgc ggcgaaaaca attggtgatt
     7801 tcgttaacgc cacgccgtca ataaaaatac tggcgttaac ctttttattg tttgtcggtg
     7861 tattgctggt tgccgatagt ctcaacattc atattgccaa agagtatctc tacttcgcga
     7921 tatttttctc attaagtgtt gagacactca atattatccg tgaaagaaaa aataaaaaag
     7981 tttcgacatt gatcgaaacg catgaatcgc actcgcatta ataaatctat taccaacttt
     8041 aagatggagt ctaactatgt ctgtagccct tattcgtgac ggcgtcatcc gtccatccga
     8101 aaacgattcg catgtgctta ccgccatgct gccctcgtcc tgcccgcaaa accacgcggc
     8161 gaatattctg cctttgccgg atggcgcgtt aatgtgcgtc tggtttgcgg gaacgcagga
     8221 aggtattgcc gatatttctg tctggggatc gcgattgccc gctggcggaa tgcagtggag
     8281 cgatgcggcc aaactttccc acgatgacac gcgttcagag caaaacccgg tgctgtttct
     8341 cgcgccggat aacgtgctgt ggctcctatg gaccgcacaa atatccggta atcaggacac
     8401 cgccatcgtc cgctaccgta agtcagacga cctcggacaa acctggggcg agattgccac
     8461 cctgcttgat aaacccggta cgttcattcg acagccaatc acggtgcttg ataacggcaa
     8521 ctggctgctg cccgtctttt attgccgtac ccaacccggc gaaaaatggg tcggcaatga
     8581 tgatatcagc gcagtgaaaa tttccgctga cggtggtcac tcctggcgcg atgtcgaggt
     8641 gccgcaaagt ctcggctgtg ttcatatgaa tatcacaatg ttgcacgacg gtacgttggt
     8701 tgctctcttt cgcagccgtt gggcagacaa catctatatc agccattcag tggacaacgg
     8761 cgagagttgg tcggtgccgc aagcaaccga attgcccaac aacaattcgt ctattcaggt
     8821 aacgacgctt gccagcggcg aactggcgct ggtgtacaac gcgatgagtg cggcaggcgc
     8881 agtagaacgt cgcgcgtcgc tttatgacga aatcgacgat ggcgacgaca gccgcaaaga
     8941 accgacagcc gtcggacgct ccgctttctg gggtgcccca cgcgcgccga tgacggtagc
     9001 gatttctgcc gatggtggga aaagctggtc gtggcggcgc aatctcgatg aaggcgacgg
     9061 ctactgcatg accaacaatt ccctggaaaa gctaaatcgt gagttctcct accccagcat
     9121 caaacaaagc ccggacggta cgctacatat cgcttacacc tggtggcgtc aggccatcaa
     9181 gtacgtacgc atctcccccg agtgggttaa ggggcaggca tcatgattat cggtaatctc
     9241 aaccatttgt cgctggcggg gcttcctgcc tgggtgagaa atattctgct gcgcccggag
     9301 tgttcgttat cggcgttatc caccagagag gacggtcgct ggcaacctga aggatgtcgg
     9361 tggttctgct cgctgggaac ttcagacacg caacccgccg aacttcgcca tacggagtat
     9421 caccatctat gggccgatat acaggtggtg attactggtt gtgagggcat taatgcggga
     9481 acgcggccca tcgctcgcga aaacgatgaa gaacgtaagc ccgatctgtt tatcgcccca
     9541 tcccctgaaa acagtgtcgc catcacccta cacgccggtg atttcgccgt ctttatgcct
     9601 gaggaacctc accaggcact gtgcgcaatt ggcacggcta cttcaatacg caaagcggtc
     9661 tttaaagtac cgcgcgacat gctggaagcc taaaaggaga gaaaaaatga caatggaaat
     9721 gatcctggca ttaggaatac tggtcctgat gatcgtgctg atcatgtcgg acaaaatgcc
     9781 ttttggcgca ccgccactgc ttgcgtgttt attgctggtt gtatcgggac tttcgaccgt
     9841 tcaacaggct tttgccggat tcgtcaatcc aagcgtggtg atgattgcag ggtttatggt
     9901 ggtcatggcg gcgctacaaa aaacccggct tatcagcaac gttaaatctg cgatgataag
     9961 cctggtcaat aaaggcagtt accgcagcta tgggctgttg ttagtcattg ttatgctggg
    10021 tgccagtctt gccggcaccg gagcgaccgg ttattacgtg ctaattctgt cgctggtatc
    10081 caccattccg tacagtaaaa agctaccaac atcgaaatta atgatgcccc tgggatttgc
    10141 caccaaccac ccgcttcttc ctatcaatct cgcgcttctc tttggcgtta cggcgaccgt
    10201 actcgaaacc gcggggtttc atcaggcgat ttcgatgggg cgctttgcgc tggtgaatct
    10261 catcatgtca gcggctttcc tcgcatggag tcttattgcc taccgttttc ttccggatca
    10321 tcccattgcg gatgcttccg aggatgcgct ggcggcacgc gaggaaacgt ttaacgccct
    10381 tcctgcctgg aaagaatatt gcactattgc cgcattcgct gtcagcgtta tcggcatgat
    10441 gctaatgaac attctgggaa atatcgcctt cgttattccg ggtcttgcgg gtgcgtttgt
    10501 tctgatgatc gatgtactgg acttcaaaga agttcgcgat catatggggg ctccggtcat
    10561 tttaatgatg gcgggggtga tcggtgtagc tgatgcactg gcgggcaccg gctttacggc
    10621 gatggttggc gatgccgttg caggtgtgct cggttctggc gtctcaccgt ttgtatttat
    10681 tgtcgccttt gctttgctta ccagtacctg cgccaccttc acgggttcga atatggggtc
    10741 agtatatatt tttgccccca tcgccattgc cgcctgtact agccttgggc ttaacccgac
    10801 cgctgccgcg attgcggtgg tgatttctgg ctggaatggc ggctatatgc ctattgacgg
    10861 aatgccagcc atgatcctcg gaatggggaa atacaaactt cccgaattct gggtgttctc
    10921 tgtaccaatg tatctcatcc gcattctggc gctttgtgca ggcgctgttt ttatcttccc
    10981 aatgtaatct cagacaaaaa aagcggcaga gaagatccct gccgcttttt attgttggat
    11041 gcgacgctgc cgcgtcgcat cagacataaa gcagattact ttttgatttc atacagcggt
    11101 gtttgacccg ccacaacatg gccctgagct ttaatgataa ggccgctgaa atcatcgata
    11161 ttgctgcaaa ccaccgggct aatcatcgaa cgggcgttag cgttcaggta atccagatcc
    11221 atttccagaa tcggttgccc tgcgcttacc tgcgcgccct cttccaccag acgtttaaag
    11281 cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc cgcgcctttt
    11341 tcggtttcca gacagaacgc gtggttggtg ttgaagattt tcacgattgt tcctgcggct
    11401 ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg
    11461 gcgaatgctt cgtcaggaat ctgatccagt gccacaacat ccccggtaat cggcgatacc
    11521 agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg agcggcagtt
    11581 gccggagttg cttcagctga cgcagcggct accggaccac gggcaacgac tttcttcatc
    11641 gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg tttgttcagt
    11701 tttactaccc cagaagcacc cagacgttta cacatcgcat cgttgacgcg agctgagtca
    11761 accacggtaa ggcgcagacg agtaatacag gcgtcaatgg ctttcaggtt gtcagtaccg
    11821 ccaaccgcag caatatagtt ggttgccagt tgattgagac cttcttcagt attgctgtta
    11881 gcctcttcag taacgatctc gtcttcttta tcttcacgac ccggcgtttt caggttgaac
    11941 atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac ccccatcacc
    12001 agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata
    12061 gcccccgcag agaaagagaa gccggcatgg atacccagca acgttgccac aaacaggctg
    12121 ataccggtca gcagtgcgtg caggaggtac agcagcggtg caaggaacat gaacaggaat
    12181 tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg
    12241 ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc
    12301 aggccgaaca tcataatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcaccg
    12361 gcatagaagc ggttgatatc accgtggaaa accgttcccg ccgcgttggt gaattcacca
    12421 atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg
    12481 ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct
    12541 gcatggatag cgtgctgtac cggtggccag acgtaaccaa aaatggccgc cagcaccaga
    12601 cagaagaagc cggtggctat cggcacaaag cgtttgccgc cgaagaagct caggaagtcc
    12661 ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata
    12721 cccgccagta cacccatgtt aatttctggg ttgatggtca ccatcgcttt ggttaacaca
    12781 aagtaaccta ccgcacccgc cagtgccgcc gcacctgcgc tgtctttcga ccagctggat
    12841 gccacgccga tggcgaatat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca
    12901 ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagcgc cgccaccggc
    12961 agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc
    13021 atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct
    13081 gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt
    13141 gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg tcaccgaata
    13201 tcgttatcat cactcccttt tactggctaa accagaaaac ttattttatc attcaaaaaa
    13261 tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat gcctaatccg
    13321 ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc tgactaccgc
    13381 tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc
    13441 gactgccgat cgtccgtttg tactgggcct gccgactggc ggcacgccga tgaccaccta
    13501 taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt
    13561 caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat
    13621 gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aatatcaacc ttctcaacgg
    13681 caacgccccg gatatcgacg ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg
    13741 aaaaattcat ctgtttatgg gcggtgtagg taacgacggt catattgcat ttaacgaacc
    13801 ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc
    13861 gaactctcgt ttctttgata acgatgttaa tcaggtgcca aaatatgccc tgactgtcgg
    13921 tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta gtcagaaagc
    13981 actggcactg caggccgccg ttgaaggttg cgtgaaccac atgtggacca tcagttgtct
    14041 gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt
    14101 taagacttta agatatttca atgaattaga agcagaaaat atcaagggtc tgtaattgtt
    14161 atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggt cagaatgtat
    14221 gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt
    14281 atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa
    14341 caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc
    14401 tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc
    14461 atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc
    14521 agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg
    14581 aaccaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc
    14641 cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgagaac
    14701 gccgacgtga ttaccaaagt gactctggca ccggaaatgg ttccagcgga agtcatcagc
    14761 aaactggcaa atgccgggat tgtggtttct gctggtcact ccaacgcgac gttgaaagaa
    14821 gcgaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg
    14881 tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat
    14941 tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgt
    15001 ctgaaaggcg acaaattgtg tctggttacc gatgccaccg cgccagcagg tgccaacatt
    15061 gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag
    15121 aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa
    15181 cattgtggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcacgtgcg
    15241 attggcgttg agaaacgtct cggcacgctc gccgcaggta aagtagccaa cctgactgca
    15301 ttcacacctg attttaaaat caccaggacc atcgttaacg gtaacgaggt cgtaactcaa
    15361 taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac
    15421 agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc
    15481 agattgccga gcaaagtcag cttgcccccg ccagcgtaac caaaattacg cgtcagctta
    15541 tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta
    15601 tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg
    15661 acgccaccat caccttgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc
    15721 tgccggaacg tacccagcag acgctggaac atgccctgct gaatgccatt gctcagttta
    15781 ttgatagcta ccagcgcaag ctacgcgagc tgatcgcgat ttcggtgatc ctgccagggc
    15841 ttgtagaccc ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg
    15901 ggctggttga agcactggaa gagcgtttta aagtgacctg ttttgtcggc cacgatatcc
    15961 gcagtctggc gctggcagag cactacttcg gtgcaagtca ggattgcgaa gactccattc
    16021 tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcga atttttatcg
    16081 ggcgcaacgg caacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct
    16141 gccactgcgg caactttggc tgcctggaaa ctatcgccgc caatgctgcc attgaacaac
    16201 gggtgttgaa tctgttaaag cagggctacc agagccgtgt gccactggac gactgcacca
    16261 tcaaaactat ctgcaaagcc gcgaacaaag gcgatagcct ggcctcggaa gtgattgaat
    16321 atgtcggtcg tcatctgggt aaaaccatcg ccattgctat caacttattt aatccgcaaa
    16381 aaattgttat tgctggagaa atcaccgaag ccgataaagt gctgctccct gctattgaaa
    16441 gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa tctaccggtg gtacgttctg
    16501 agttggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg
    16561 gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg
    16621 tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct
    16681 gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa
    16741 aggcctgccg ctggtgttgc tgaccaacta tccttcgcag accgggcaag atctggcgaa
    16801 tcgctttgcc actgccggtg tcgatgtacc ggatagcgtg ttttatacct ccgcaatggc
    16861 gaccgccgat tttctgcgtc gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc
    16921 actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc ctgattttgt
    16981 gattgttggc gaaacgcgtt cctacaactg ggacatgatg cataaagcag cctatttcgt
    17041 cgctaacggt gcacgcttta tcgccaccaa cccggacacc cacgggcgcg gtttttatcc
    17101 cgcctgtggc gcgttgtgtg cagggatcga gaaaatctcc gggcgcaaac cgttctatgt
    17161 tggaaagccg agtccgtgga tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga
    17221 agaaacggtg attgtcggcg ataacttgcg taccgatatt ctggcgggct tccaggccgg
    17281 tctggagacg attctggtgc tttctggtgt ttcgtcgctc gacgatatcg acagtatgcc
    17341 gttccgccca agttggattt acccatcggt cgctgaaatc gacgttatct gaatagcaac
    17401 cacgcctcag ggcgtggttt cttattttta cccatcatta aaatcacccc attcaataaa
    17461 acccgtcaaa aactatcgat ccatcaataa atatacccaa aagccctccc ctttttcatc
    17521 aatcagcaat cgccattacg ttttttattt ttccactgcc aaatcgcttg cgcaccgtgc
    17581 tgctttgcgg cattttttta acaagtaaac acaacacgca gtaaacacca ggttaatgga
    17641 gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg
    17701 taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat
    17761 ttatgccagc gataacgcca ttctcgccca cgaacgtctg tcaattgttg acgttaacgc
    17821 gggggcgcaa cctctctaca accaacaaaa aactcatgtg ctggcagtaa acggtgaaat
    17881 ctacaaccac caggcattgc gcgctgaata tggcgatcgt taccagttcc agaccgggtc
    17941 tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt
    18001 gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcatatc tgattggtcg
    18061 cgaccatctg gggatcatcc cactgtatat gggctatgac gaacacggtc agctgtatgt
    18121 ggcctcagaa atgaaagccc tcgtgccagt ttgccgcacg attaaagagt ttccggcggg
    18181 gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga
    18241 ctacgatgcg gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg cactggaaga
    18301 ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctgcttt ctggtggtct
    18361 ggattcctca attatttccg ctatcaccaa gaaatacgcc gcccgtcgcg tggaagatca
    18421 ggaacgctct gaagcctggt ggccgcagtt gcactccttt gctgtaggtc tgccgggttc
    18481 accggatctg aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat
    18541 tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac
    18601 ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa
    18661 ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gacgaagtgt ttggcggtta
    18721 tctctacttc cacaaagcgc cgaacgctaa agagttgcat gaagagacgg tgcgtaaact
    18781 gctggccctg catatgtatg actgcgcgcg tgccaacaaa gcgatgtcag cctggggcgt
    18841 ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc
    18901 acaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga
    18961 agcgtatctg cctgcaagcg tggcctggcg gcaaaaagag cagttctccg atggcgtcgg
    19021 ttacagttgg atcgacaccc tgaaagaagt ggcagcgcag caggtttctg atcaacagct
    19081 ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag cgtatctgta
    19141 ccgggagatc tttgaagaat tgttcccgct tccgagcgcc gctgagtgcg tgccgggcgg
    19201 tccttccgtc gcttgttctt ccgctaaagc gattgagtgg gatgaagcgt tcaagaaaat
    19261 ggacgatccg tctggtcgcg cggttggcgt tcaccagtcg gcatataaat aagacgtgta
    19321 atattgcccc ggagaaatcc ggggcaattt cagttactgg ggcgcttgtt gattctgata
    19381 tgcagtaagg aaatgttccg ggtttgccac atcgctattt gacaactcat cactgtaggc
    19441 caccaaccag gtcgcctgct catccggaat atgcagtctg ccatttccct ctttcaggcg
    19501 taataactca aaagatttgc ccttaattac ggcggtatag gatttctcac cttcctgtaa
    19561 tgctttcact ttacagcgaa atatctgacc atcgatgtca ttatcaacat gcgatacctg
    19621 aatgagggct tcattagaag ctttcgggcc tatccgcatc atccacacca cagcgccttc
    19681 ctgcccacga tagccaaaaa catatttacc gatttccggt ttaaccgtat cggcgaacgc
    19741 agattgcata agtaataacg cacccatcaa cgccgtcata aaacgcagca tagtattccc
    19801 tcaataaatc gcctgaagtg gtagcagaga tgcaggcgta gcgatccctg ccctgtcctg
    19861 ttgcaacggg ccagtatttc atagattaat ttcccaccac tattgttgaa ttccgaattt
    19921 catcctgtat gacaaaagaa tggctgtagg gtggggaaaa aacacaaagt aaacaataat
    19981 tgacgaatat agcgccatgc tgttcgcaac ctaaccaaac agtcactttc gagcaatttt
    20041 ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat aatgcgcccc gcaacgccga
    20101 taaggtatcg cgaaaaaaaa agatggctac gtagctcagt tggttagagc acatcactca
    20161 taatgatggg gtcacaggtt cgaatcccgt cgtagccacc atcttttttt tgcgggagtg
    20221 gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt
    20281 ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa
    20341 ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca gccatattct
    20401 tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc aagcggtaag gcaccggttt
    20461 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcgaaga aacaatctgg
    20521 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    20581 ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc ggcaaattca aaaaccaatt
    20641 tgttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc cgaggttcga
    20701 atcctcgtac cccagccaat ttattcaaga cgcttacctt gtaagtgcac ccagttgggg
    20761 tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt
    20821 accccagcca cattaaaaaa gctcgcttcg gcgagctttt tgcttttctg cgtatattca
    20881 atgccgaata cgatgttgac tcgtcttatc cttacaaagt cggatgcgac gctgccgcgt
    20941 cttatccgac ctacggttgg catgcatcca gcaatgttgt gaggctacaa ccctaacgca
    21001 tatttcagcg ccttacgttt caacacgcca gcacgctccg ccgccatcaa accaagatta
    21061 cgcacaaaac gcagcggtgg cagattattg ctgaatccgg cataaaacag atccatacca
    21121 ctttgcatca tgaagttatc cgccatacgc cgcatctggt aacgcttgag cacaggataa
    21181 ctggcccacg cttcgccgta gctgcgggcg ttcaccagaa cgtcaatcag ggcatcgaca
    21241 tcacgataac caagattcac tccctgcccc gccagcggat ggatggtatg cgcggcatcg
    21301 cccaccagag caagccctgg ttgcacatac tgcaacgcat ggcgacgtat aagcggaaac
    21361 gcaccagcgg caagcggtgt cacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc
    21421 gcctgtagct gcgccatatt catattctgc aactggcgaa tgcgcgccgg agagtcatac
    21481 cacaccagcg atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggggta
    21541 aattgctgcc aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatacac
    21601 gactgcgcat actgccaggc atgaacgcca attcccgcca tctgtcgcac ctgcgaattt
    21661 gcgccgtcgg caccaatcac cagcttcgcg aggatcgttt caccgccctt cagttccagc
    21721 tcctgaagat cattatggcg atgcagtgca gtcagcgagg ttggcacacg taacgttact
    21781 ttcggatgcg cttccagcgc ctgccacaaa gcctgttgca ggacagtgtt ttccaccatg
    21841 tagccaagca acggtagctt aagttcagcg gcgtcaaaca cgacatgcgc cgtttcccac
    21901 tcccacgttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc
    21961 cctaaccctt taagcaatga taccgaagcc gcgctgatcg ccgagatccg cacatccggt
    22021 tggctgtcag cgacaaacgg cgcaggttct gcgtgctcga tcaccgttac cgtaaacccg
    22081 tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt
    22141 tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg
    22201 atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca
    22261 ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc
    22321 catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg
    22381 ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg
    22441 ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc
    22501 tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggcgg ctgcgtggca
    22561 tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg
    22621 caaacgctgc atcgtctacc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt
    22681 gtagatatca gcttcccgga aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa
    22741 gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgtg
    22801 gtgccttaca cccgtggtga agaggtaagc cgtccgtccg acgatattct gtttgagatc
    22861 gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc
    22921 tggcgtggtg agaactacga cggcaccacc ggaacgtttg ccgatctgct gcgtctggtt
    22981 gcggcgattg acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc
    23041 gacgatatca tcgaagtgta tcgcgacacg ccggagctgg tgagcttcct gcacttgccg
    23101 gtacagagcg gttccgatcg cattctgaac ctgatgggcc gtactcacac ggcgctggag
    23161 tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccgg atattcagat cagttctgac
    23221 ttcatcgttg gcttccctgg cgaaaccacc gacgacttcg agaaaacgat gaagctgatt
    23281 gccgacgtca atttcgacat gagctacagc tttatcttct ccgctcgtcc aggaacgcct
    23341 gccgccgata tggttgatga tgttccggaa gaagagaaga agcagcgtct gtatattctg
    23401 caagagcgca ttaatcagca agcgatggca tggagccgcc gtatgctcgg caccacccag
    23461 cgtattctgg tagaaggtac atcgcgtaag agcatcatgg agctttccgg tcgtactgaa
    23521 aataaccgcg tggttaactt cgaaggcacg ccggatatga tcggtaaatt cgtcgatgta
    23581 gaaattaccg acgtctatcc aaactccctg cgcggaaaag tggtgcgtac tgaagatgaa
    23641 atgggtctgc gcgtcgcaga aacaccggaa tcggtgattg cccgtacccg caaagaaaac
    23701 gaccttggcg tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg
    23761 ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga
    23821 ataatttcct tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg
    23881 gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaatgcg cgtctgttga
    23941 gcctgtgcgg cccgtttgat gacaacatca aacaactcga acgccgtctc ggcatcgaaa
    24001 tcaatcgccg tgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg
    24061 cagacattct gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag attcaggata
    24121 tcgaaccgga acagatccac cttgcgatta aagaagcgcg ggtactggag caaagcgcgg
    24181 agagcgtgcc ggagtacggc aaagcagtca atatcaaaac caaacgcggc gtaattaagc
    24241 cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg
    24301 gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc
    24361 tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga
    24421 aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt
    24481 acgacgcgct gtttgaaatg ctgggctttg agaaagtcga gaaactgatt gagcgcaacg
    24541 ttattgaagt cgcaccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca
    24601 ttcttgatga gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg
    24661 gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata
    24721 ccaagtctgg tttgcgtcat gccatcgaag tgctggctga tgtcgaagag atcagcttta
    24781 acttcttcca cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt
    24841 atgaagcctg ggaagaagcc gaacaaaaac gtaaagcggc gctggcggca gaacgcaagc
    24901 gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga
    24961 taattccggg ttaccggaag agagccagtt tcagacatgg ctgaatgcgg tgatcccgca
    25021 gtttcaggaa gaatcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct
    25081 gaatctgacc tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt
    25141 gccgcctggc atggaaatgt cgctactggg cgatctggtt atctgccgtc aggtggttga
    25201 gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca
    25261 cggcagtctg catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga
    25321 agccatcgaa acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa
    25381 agaataattt gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc
    25441 gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt
    25501 agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac
    25561 ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac
    25621 cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc
    25681 gttcgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg
    25741 gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa
    25801 gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc
    25861 gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt
    25921 aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtc
    25981 attgacgaat tcggtggggt ttccggtctc gtgaccattg aagacatcct ggaactgatt
    26041 gttggtgaga ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt
    26101 cgtcatacct ggaccgtgcg cgcactggct tctattgaag acttcaacga agcgttcggc
    26161 acccacttta gcgatgaaga ggttgacact atcggtggtc tggtgatgca ggcatttggg
    26221 catcttccgg cgcgtggcga aaccatcgac atcgacggtt accagttcaa agtggcgatg
    26281 gccgacagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagccg
    26341 aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt
    26401 gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc
    26461 ttctctcctt acgacgtctg gcctgcggcg attatttcgc tgatgggact tcaggcgctg
    26521 acctttaacc gccgtccact ccagtctacc gctattggct tttgctgggg atttggcctc
    26581 tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg aatgcctggc
    26641 ccggttaaca tcttcctggt ggtactgctg gcggcgtatt tgtcgctgta taccggactg
    26701 tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc
    26761 gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt gggtactaac aggcttcccg
    26821 tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc accgataatg
    26881 ggcgtggaag ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg
    26941 gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct tcccttcccg
    27001 ctgcgttacg tccagtggtt taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt
    27061 cagggcaata ttccgcaatc gctgaaatgg gacgaaggcc agcttcttaa tacgctgaag
    27121 atttactaca acgcaacggc accgctgatg ggcaaatcat cgctgattat ctggccggag
    27181 tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagctct ggacggtgag
    27241 ttgcgcgata aaggtagctc gctgatcacc gggattgtcg acgcgcgtct caataaacag
    27301 aaccgctacg atacctacaa caccatcatc acactgggta aaggcgcacc gtacagctat
    27361 gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccactg
    27421 gagtcgattc tgcgtccatt agcaccgttc tttgatttgc cgatgtcgtc attcagccgc
    27481 ggaccatata ttcagccacc gctgtcgttg aatggcattc aacttactgc ggccatttgc
    27541 tacgaaatca ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg
    27601 ctgactatct ccaacgatgc gtggtttggt aagtctattg gtccatggca gcacttccag
    27661 atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc
    27721 attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc
    27781 gaggtgttaa ccactaacgt aacgccgacc accggactca ccccgtactc acgtaccggc
    27841 aactggccac tgtgggtgct gacggcactg tttggctttg ccgctgtact gatgagtctg
    27901 cgtgctcgca aacgttaatg tgcctgatgc gcgacgctta tcaggcctac gcgatttttg
    27961 caatatgttg aattttcgcg attttgtagg ataaagcgta ctttttcaac acgctctggg
    28021 gcatgattat catgcccttt tttcttacgg ttcagggata caagccacat cgccatattg
    28081 gggatgccga ataattgcct gcggatcaag tgaactttct ctgtccagtt gtattagccc
    28141 gttcttcgcc ataacaaata tctttctacc atccgctgaa aaacgaagat gattcacgta
    28201 gctggcagga taaacgccaa taatctgccc ggcattaata ttgagcagga tgacataacc
    28261 ttgcaggcca tatgacacca acgctaaata ttgaccgtcc gggctaatac cctcaacgga
    28321 ataaacgctc ctgctacacc attgcgtcat aatgctggct tgttgatcct cagctcttat
    28381 acgtgaaatc ccctggctat cggcaaaaat aatctcacca ttttttggtc ggaatcccat
    28441 cgcagaaacg acaccacgcg catcacctgc gggaaagtct tctttctgat gcgtgatggt
    28501 attgatccgc acgatgttac ctctgcctgc tcccggaacc agtaatgttt tttcatccgg
    28561 ggaaagtgcc ataacccagg cgcctggttt accgctcatc cccgtcccgt cgtatttctc
    28621 cagcgggata tccgccacat gctgataagc caccagatca atcgctgaca cactattact
    28681 ggtgcgattc atcacccagg cttttgtgcc atcacgcgag aggataaggc tgtcaggatg
    28741 tgcgccggtg gtaatcgact gaataatctc ccccgttgcc acgttaatga ccgataatgc
    28801 attcgcttca aatgaagtcg catacagcaa cgtttcatcc gggcttagcg ccagcgacct
    28861 gacgccctga tctttacagc caccacaatg tttgactgac ttttctttcg ctatgggggc
    28921 tgtaaaggta tgcaggattt tcttttgtcc gaggtcgaaa acaacaatgc tgttcgtgtt
    28981 ttcaattgcg gcatacaacg tattagcatc ccgactgatc gccatatcat ggatatcggc
    29041 aatctcagcg agtaaatcac cactggcctg ctcacgactt accggagcgg ataatgtttc
    29101 cctgagggcg ttgcgctcct ccactaactg ctgctttttt tgtcgtcgct cttgttcaat
    29161 ctcctgcgga taaaacgctt cacgcaggct ttgcctgacc tgtaaaactg attcatcaat
    29221 ctgctgctgc acaagccaga tagtcccggt cagaagaaga gtgaccgcaa caataatccc
    29281 gccttccgta acgagccgat tttgcccaac taaacgcgca ctcagccaac ccaacgcgcc
    29341 ccccgcaatc aggccgccaa tgtggcaagc gttatctatt cctgtttgcc gggtaccata
    29401 caacagtgtc agcgccacca tacccaacag aggaaatact cgccgctggt ttttatggtg
    29461 tgtgcccgca cctgaagcaa gctgtgttgc tatcgcagcg cccgctattc ccataattgc
    29521 cccggatgcc ccaacgctga tgactatctg ctcactttcg cgtaacgcat aacaggcgct
    29581 gatcagccca ccaaagatgc cggagaataa ccagataatc aggagtcgcc atttccccag
    29641 tatccgttcc gccactgtcc ccaccaccag gagtgcaaac atattcatca gcaaatggga
    29701 aaaactactg tgcagaaaaa cactgctcaa taagcgccag gattcatccg acaacgtgag
    29761 agaagcaacg ttagcgccga gatggatgag tgcggattga tcgacttctt cgtaactcat
    29821 ccattgtgcg aggtaacaaa atatcccaac acagagcgca aaaaggatca gggttattgc
    29881 gggtaattga acatttaacg gcttaaccga agatgcagac ataaggtgat tcttccttga
    29941 aaagactagt aaaaagcatt ttcccgtgtg tagttatcat cccggcaaac gtcccacacc
    30001 gaaaagttaa cactgtccga aatatgactt tttcaaactg aaattacaat cgtaattaat
    30061 tcacccctaa gtattttttg gcatgtaagt tgctttattt actgtgtaag caatgaaatc
    30121 agccgcaacg caaccgggtt gcaccaccac aaatcagcgg tagcaccaaa aaggtgcggt
    30181 ttaagaattt tgcttcggaa tggtgcggcg cacctcgcaa aaacaaacaa taaaacagca
    30241 atatctttac atttatccaa actaaatgtt acctaacaag cacaacactg cacaataaag
    30301 tcgcagatga taacaacaca aacactcaca acgggtatcc atgcgttttt aacgcagaag
    30361 ataaaggagt tggatatgca attacgtaaa cctgccacag caatcctggc cctggcgctt
    30421 tccgcaggac tggcacaggc agatgacgcc gccccggcag ctggcaatac gctggacaaa
    30481 atcgccaaaa acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat
    30541 tacgacaatc agcaaaaagt ggtgggctac tcgcaggatt actccaacgc cattgttgaa
    30601 gcagtgaaaa agaaactcaa caaaccagac ttacaggtga aattgattcc gatcacctca
    30661 caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgcgg ttctaccacc
    30721 aacaatgccg aacgccaaaa acaggcggct ttctctaaca ctattttcgt ggtcggtacg
    30781 cgcctgctga ccaaaaaggg cggtgatatc aaagattttg ccgacctgaa aggcaaagcc
    30841 gtagtcgtca cttccggcac cacctctgaa gttttgctca acaaactgaa tgaagagcaa
    30901 aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg taccctggaa
    30961 agcggtcgtg ccgttgcctt tatgatggat gatgctctgc tggccggtga acgtgcgaaa
    31021 gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc agtctcagga agcctatggt
    31081 tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgatac catcgctcag
    31141 gtgcagacct ccggtgaagc ggaaaaatgg tttgataagt ggttcaaaaa tccaattccg
    31201 ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa
    31261 ccgaatgaca aggcactgaa ctaattacaa gaaccagggg cggaaattcc agccctctcg
    31321 attgttacgt agcacggaca gactatacgc ctggtggtcg ttccccatcg ggcctgaaaa
    31381 ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt
    31441 ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt
    31501 cggcaacacc acctatcttg gttggatctg gagtggcttt caggtgacga tcgctttgtc
    31561 gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt tacgtaccgt
    31621 tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc
    31681 gttgattgtg caattcttta cctggtacct agtgatcccg gagctgctgc cagagaaaat
    31741 cggcatgtgg tttaaggcag agctggatcc caatattcag tttttccttt catccatgct
    31801 ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgcag ctattcagtc
    31861 gctgccgcgt gggcaaaaaa atgccgcgct ggcgatgggt ctgacgctac cgcaggctta
    31921 ccgctacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga cctcagagat
    31981 gatgaacctg gtgaaaaact ccgccatcgc ctcgactatc ggtctggtgg atatggcggc
    32041 gcaggcgggt aaattgctcg attactcggc ccacgcctgg gagtcgttta ccgccatcac
    32101 gctggcgtat gtattgatta acgcttttat catgctggtg atgacgctgg ttgaacgtaa
    32161 agttcgcctg cccggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt
    32221 gttccttccc tgccatatct gctcgacggg ctggtgatca cgctgaaaat caccgtcacg
    32281 gcagtggtga ttggtatttt gtggggcacc atgctggcgg tgatgcgctt gtccagcttt
    32341 gcgcccgtag cgtggtttgc caaagcctac gttaacgtat tccgctcgat tcctttagtt
    32401 atggttttgc tgtggtttta cctgatcgta ccgggttttc tgcaaaacgt gctgggatta
    32461 tcacccaaaa atgatatccg tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg
    32521 gcctactatt cagagattat ccgtgctggt attcaaagta tttctcgtgg tcagtcgagc
    32581 gccgcgctgg cgttgggaat gactcactgg cagtcgatga aactgattat tctgccgcag
    32641 gcgttccgcg cgatggtgcc gctgttgctc actcagggca tcgtactgtt tcaggatacc
    32701 tcactggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag
    32761 cgtgatggta cgcaggttga gatgatcctg tttgccggat ttgtttattt cgttattagc
    32821 cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg attaccctga
    32881 aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga
    32941 aaaaaggcga agttgtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta
    33001 aaaccgtcaa cggcctcgaa ccggtgcagc aaggtgaaat caccgtcgat ggcatcgtgg
    33061 ttaacgacaa gaaaaccgat ctggcgaaac tgcgttcccg cgtcgggatg gtgtttcagc
    33121 atttcgagct gttcccccat ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag
    33181 tgcttaaacg caataaagcg ccggctcgtg aaaaagcatt gaaattgctg gagcgcgtcg
    33241 ggctttccgc acatgccaat aagtttccgg cgcagctttc cggcggccag cagcagcgtg
    33301 tggcgatcgc tcgcgcattg tgcatggatc ctattgccat gctgttcgac gaaccaacgt
    33361 cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtcgaa ctggcgaacg
    33421 aaggaatgac gatgatggtg gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc
    33481 gggtgatctt tatggacgag ggtaaaatta tcgaagactc gccgaaagac gctttcttcg
    33541 ataatccgaa atcggaccgc gcaaaagact tcctcgcaaa aatcctgcat taatcactct
    33601 ggcgcgcata ctcaattgcg cgccatctca cgctttctcg tttcgtccct cgtatctgtt
    33661 acatcccggc gttacccttg tcgcaaagaa acacacaaca aggagcaaca atggcactgc
    33721 caattctgtt agattgcgac ccaggtcatg acgacgctat cgcaatagtt ctcgccctcg
    33781 cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa
    33841 aaaccttacg caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtag
    33901 caagcggtgc ggtaaaaccg ttaatgcgta atttgattat tgcggacaac gtgcacggcg
    33961 aaagcggcct ggacggcccg gcattgccgg aaccggcatt tgcaccgcaa aactgcacgg
    34021 cagtggagct gatggcgaaa acgctgcgtg aaagtgcgga acctgtcacc attgtttcta
    34081 ccggaccgca aactaacgtt gccttgctgc tcaatagcca cccggaactg catagcaaaa
    34141 ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg cctgcggctg
    34201 aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca ggaattccgg
    34261 tggtgatggc cggtctggat gttactcaca aagcacaaat ccacgttgaa gacactgagc
    34321 gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc
    34381 tcgaatatca caaagacgaa aaatggggct ttgtcggcgc accactgcat gacccatgca
    34441 ccatcgcctg gctgttgaaa ccggagttgt ttaccactgt tgagcgctgg gttggcgtgg
    34501 aaacacaagg gaaatatacc cagggtatga cagttgttga ttattattat ctgactggca
    34561 ataaaccgaa tgccaccgta atggtcgatg ttgatcgtca gggctttgtt gatttactgg
    34621 ctgatcgtct gaaattttac gcttaacata cgtcattgat taagatcacc acgcattacc
    34681 tgggtggtct taatctggtt atctcttttt ctttcaagat ataaatgcaa tggataatgc
    34741 agaacttgct attggtatcg atctcggtac taccaatagt ttaattgccg tctggaaaga
    34801 cggtgccgcg caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag
    34861 catggatgaa aataatcata ttttagtcgg aaaaccggct gtatcacggc ggacttcgca
    34921 tccggataaa acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt
    34981 aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga
    35041 agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg ttcctgctta
    35101 tttcagcgat gaacaacgca agcatacccg tttagcagca gagttagccg ggctaaatgc
    35161 agtgcgcctg attaatgagc caacggcagc cgcgatggcg tatggcctgc atacgcagca
    35221 aaatacccgc tcgctggttt ttgatctcgg tggcggcacg ttcgacgtta cggtgctcga
    35281 gtacgccacg ccggtgattg aagttaacgc ctccgctggc gataacttcc tcggtggcga
    35341 agattttacc catatgctgg tcgatgaggt tttaaaacgc gcggaagttg ccaaaaccac
    35401 gcttaacgat agcgaactgg cagccttgta cgcctgcgtg gaagcggcaa aatgtagcaa
    35461 tcaatcgcca atgcacattc gctggcagta ccaggatgaa atgcggaaat gcgaatttta
    35521 cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag tgccgattga
    35581 acaggcgttg cgcgatgcac gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg
    35641 cggcgcgtca caaatgccgc tggtgcagcg aatcgcagtg cgtctgtttg gcaaattacc
    35701 gtatcaaagt tacgatccga gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg
    35761 ccgcttacgc aatgaagata ttgaagaagt gatcctcact gatatttgcc cttactcgtt
    35821 gggtgttgaa gttaaccgtc agggcgtatc cggcattttc tcgccgatta ttgaacgaaa
    35881 caccacagta ccggtgtcgc gtgtagaaac ttattcaacc atgcacccgg aacaggattc
    35941 aattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaataaca ttcttgtgga
    36001 atccttcgat gtgccgttga agaaaaccgg gacttatcag tcgattgata ttcgctttag
    36061 ttatgatatc aacggcttgc ttgaagttga cgtgcttctg gaagacggca gcgttaagtc
    36121 cagagtgatt aatcacagtc cggtaacatt gagcgcgcag cagattgaag agagtcggac
    36181 gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaatcgca cctttaaagc
    36241 caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga ttggtcgggt
    36301 gatcaccgat tttgatgcgg cgttgcagtc aaacgatatg gcccgcgtgg atgaagttcg
    36361 gcggcgggcg agcgattatt tagccattga gatcccataa acacggctac cggatgcggc
    36421 gtaaacgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca acatcgcgat
    36481 tttgccgaga gcataaccta tcaccagcac cgttaaactg attttgataa tgctggtaat
    36541 gctctgtggt ttttcatcct catcatttat ttcttcatgg ttttcaggtg ttggctcatc
    36601 gacatcttca tcctccggct cgagcttcac tttcgtcaac agcattcggg tgataatggt
    36661 ctctaatgga tgtgcctctc cgcgttccag ccgcgcgtcc agacgggcca gcatctcttg
    36721 tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca gtaacggtga
    36781 atcttgctct gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgtttgaa
    36841 gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat gtaaaatctc
    36901 cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcaacg gcttaccttg
    36961 cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat aatccgggca
    37021 atgacgcttg ctccactgca acaacaaatt atcggcttgt gtagatgggt aactttcccg
    37081 gtacgcgcag agatcggcaa gcaggctttc cccctcgccg cagtacaacc gttgtgcgca
    37141 ctggtaataa cgggcatctt cgctttctgg ttcttccgct tgccactgtt gagcgacagg
    37201 aatgagttcc gccatacctg tttgcaccga gctgtaccag cgcaacagtt tgcgatgcaa
    37261 cttcgcatca tcaggaatga accacggacc ttccatcgcc aaaaacgcag tgacatagtc
    37321 aggatggtaa cggcagcaac gttccagcga gaagtaatac tcaaccgtct ggttctgcaa
    37381 cgcaacggga tattccagaa ggctaaggaa atcgaaacaa tcaccagcct gaatggcctc
    37441 aagaaaagcc tccctttctt cctcatctat tccttcgcag tcattaaacg aatgccagtt
    37501 caaacgctct gccagcaaag aagcacaatt taatgaaaga tatcgcgctt ctatagcgat
    37561 tgcacacagc ggccagcgta attgatcgac atcctccatg ttccaggtat ttaattgctg
    37621 aataaatttc tgccaggcgg agggctgaaa acgatcattt tctgaatcca gtaatgccct
    37681 gaatacacgt agtatttcat gctcagtggc agcatctttt tcttcatcat ctgcttcctc
    37741 gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg ctttaaaccc
    37801 ttgtggatcg gtttcgggat gacataacgg taagcgagca agataagcct ggcggataat
    37861 gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc
    37921 acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca gggaagcgtg
    37981 gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca tccgactccc ggcgctatcg
    38041 tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg agaagattgc
    38101 cagtagagac gccctaacga ataggcagaa aaatagtgca tccagctgtg atagtaatgg
    38161 tgtgctcgta tatagacgcg cgattgcagc cataaactct cgtcttccgt caaccacttg
    38221 ttgagcacac caacccgtga taaaaaaccc atccgcacat aatcccaggc tttgattccc
    38281 ccttccccac aacacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc
    38341 gagccgccag caattaatgc tttccactct tcaggggaag agcgatgcca tgtcagatat
    38401 aagccagcta aatcattggc atgaccatca tcggtcatct gataaatagt ctgaattaaa
    38461 tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat cccggtatac
    38521 atttttggcg agtcatgcgc gggcttgcaa ccctgattaa gtatttccat gggcgtagaa
    38581 agcgcaaaaa gaagatattg ttcttcctta ttcatttgt
//
LOCUS       DAJOTB010000027        37856 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJOTB010000027 DAJOTB010000000
VERSION     DAJOTB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.027"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(174..332)
                     /locus_tag="OHN28_001424"
     CDS             complement(174..332)
                     /locus_tag="OHN28_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296843.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
     gene            complement(938..1013)
                     /locus_tag="OHN28_001425"
     tRNA            complement(938..1013)
                     /locus_tag="OHN28_001425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Thr,seq:cgt)
     gene            complement(1128..2381)
                     /gene="proA"
                     /locus_tag="OHN28_001426"
     CDS             complement(1128..2381)
                     /gene="proA"
                     /locus_tag="OHN28_001426"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCQ2296844.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2393..3496)
                     /gene="proB"
                     /locus_tag="OHN28_001427"
     CDS             complement(2393..3496)
                     /gene="proB"
                     /locus_tag="OHN28_001427"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCQ2296845.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3785..4840
                     /gene="phoE"
                     /locus_tag="OHN28_001428"
     CDS             3785..4840
                     /gene="phoE"
                     /locus_tag="OHN28_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCQ2296846.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4879..5280)
                     /gene="crl"
                     /locus_tag="OHN28_001429"
     CDS             complement(4879..5280)
                     /gene="crl"
                     /locus_tag="OHN28_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCQ2296847.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5338..6582)
                     /gene="frsA"
                     /locus_tag="OHN28_001430"
     CDS             complement(5338..6582)
                     /gene="frsA"
                     /locus_tag="OHN28_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCQ2296848.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPCLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6674..7132)
                     /gene="gpt"
                     /locus_tag="OHN28_001431"
     CDS             complement(6674..7132)
                     /gene="gpt"
                     /locus_tag="OHN28_001431"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2296849.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7393..8850
                     /gene="pepD"
                     /locus_tag="OHN28_001432"
     CDS             7393..8850
                     /gene="pepD"
                     /locus_tag="OHN28_001432"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCQ2296850.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8906..9520)
                     /locus_tag="OHN28_001433"
     CDS             complement(8906..9520)
                     /locus_tag="OHN28_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCQ2296851.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9517..10656)
                     /locus_tag="OHN28_001434"
     CDS             complement(9517..10656)
                     /locus_tag="OHN28_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCQ2296852.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10846..11298)
                     /locus_tag="OHN28_001435"
     CDS             complement(10846..11298)
                     /locus_tag="OHN28_001435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2296853.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11295..12350)
                     /gene="dinB"
                     /locus_tag="OHN28_001436"
     CDS             complement(11295..12350)
                     /gene="dinB"
                     /locus_tag="OHN28_001436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCQ2296854.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12421..13344)
                     /gene="lafU"
                     /locus_tag="OHN28_001437"
     CDS             complement(12421..13344)
                     /gene="lafU"
                     /locus_tag="OHN28_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCQ2296855.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(13347..14210)
                     /gene="motA"
                     /locus_tag="OHN28_001438"
     CDS             complement(13347..14210)
                     /gene="motA"
                     /locus_tag="OHN28_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCQ2296856.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14223..14939)
                     /locus_tag="OHN28_001439"
     CDS             complement(14223..14939)
                     /locus_tag="OHN28_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HCQ2296857.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(14959..15426)
                     /locus_tag="OHN28_001440"
     CDS             complement(14959..15426)
                     /locus_tag="OHN28_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HCQ2296858.1"
                     /translation="MKKIVVAGVVSVVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(15434..16495)
                     /locus_tag="OHN28_001441"
     CDS             complement(15434..16495)
                     /locus_tag="OHN28_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCQ2296859.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16492..16803)
                     /locus_tag="OHN28_001442"
     CDS             complement(16492..16803)
                     /locus_tag="OHN28_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HCQ2296860.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(16808..17200)
                     /gene="fliS"
                     /locus_tag="OHN28_001443"
     CDS             complement(16808..17200)
                     /gene="fliS"
                     /locus_tag="OHN28_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCQ2296861.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17223..18539)
                     /gene="fliD"
                     /locus_tag="OHN28_001444"
     CDS             complement(17223..18539)
                     /gene="fliD"
                     /locus_tag="OHN28_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCQ2296862.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(18949..19863)
                     /gene="lafA"
                     /locus_tag="OHN28_001445"
     CDS             complement(18949..19863)
                     /gene="lafA"
                     /locus_tag="OHN28_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HCQ2296863.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            20349..21191
                     /locus_tag="OHN28_001446"
     CDS             20349..21191
                     /locus_tag="OHN28_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCQ2296864.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEALIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(21250..22227)
                     /locus_tag="OHN28_001447"
     CDS             complement(21250..22227)
                     /locus_tag="OHN28_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HCQ2296865.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAVRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQILLQAVNGQARISSQTVRNLLG"
     gene            complement(22244..23173)
                     /gene="flgL"
                     /locus_tag="OHN28_001448"
     CDS             complement(22244..23173)
                     /gene="flgL"
                     /locus_tag="OHN28_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCQ2296866.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLVVRDDLLEAANGTN
                     TADSLASLGQDIESLTDSMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(23188..24564)
                     /gene="flgK"
                     /locus_tag="OHN28_001449"
     CDS             complement(23188..24564)
                     /gene="flgK"
                     /locus_tag="OHN28_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCQ2296867.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(24754..25053)
                     /locus_tag="OHN28_001450"
     CDS             complement(24754..25053)
                     /locus_tag="OHN28_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HCQ2296868.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMLVKQLSPRHK"
     gene            complement(25053..26153)
                     /locus_tag="OHN28_001451"
     CDS             complement(25053..26153)
                     /locus_tag="OHN28_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCQ2296869.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMTATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(26168..26833)
                     /gene="flgH"
                     /locus_tag="OHN28_001452"
     CDS             complement(26168..26833)
                     /gene="flgH"
                     /locus_tag="OHN28_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCQ2296870.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(26916..27701)
                     /gene="flgG"
                     /locus_tag="OHN28_001453"
     CDS             complement(26916..27701)
                     /gene="flgG"
                     /locus_tag="OHN28_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCQ2296871.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(27780..28517)
                     /locus_tag="OHN28_001454"
     CDS             complement(27780..28517)
                     /locus_tag="OHN28_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HCQ2296872.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(28517..29719)
                     /gene="flgE"
                     /locus_tag="OHN28_001455"
     CDS             complement(28517..29719)
                     /gene="flgE"
                     /locus_tag="OHN28_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HCQ2296873.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(29991..30704)
                     /locus_tag="OHN28_001456"
     CDS             complement(29991..30704)
                     /locus_tag="OHN28_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HCQ2296874.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(30704..31135)
                     /gene="flgC"
                     /locus_tag="OHN28_001457"
     CDS             complement(30704..31135)
                     /gene="flgC"
                     /locus_tag="OHN28_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCQ2296875.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(31138..31473)
                     /gene="flgB"
                     /locus_tag="OHN28_001458"
     CDS             complement(31138..31473)
                     /gene="flgB"
                     /locus_tag="OHN28_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCQ2296876.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            31555..32292
                     /gene="flgA"
                     /locus_tag="OHN28_001459"
     CDS             31555..32292
                     /gene="flgA"
                     /locus_tag="OHN28_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCQ2296877.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGETV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            32374..32652
                     /gene="flgM"
                     /locus_tag="OHN28_001460"
     CDS             32374..32652
                     /gene="flgM"
                     /locus_tag="OHN28_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HCQ2296878.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            32665..33093
                     /locus_tag="OHN28_001461"
     CDS             32665..33093
                     /locus_tag="OHN28_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HCQ2296879.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRAREDL
                     LAVNDEITQHYEQLHAHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            33221..34153
                     /locus_tag="OHN28_001462"
     CDS             33221..34153
                     /locus_tag="OHN28_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HCQ2296880.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRQSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQAILSTLTLPDLTPELYLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQPARQLMTLLG
                     SCAFDWSILPESPRKTSLPLQMPLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRESENAVLIRQQIQAERSADPLLYAFSLLSC"
     gene            34200..36638
                     /locus_tag="OHN28_001463"
     CDS             34200..36638
                     /locus_tag="OHN28_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2296881.1"
                     /translation="MQTLIKTLVTHRVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAVLTDIVSDPLLRNAYLGLHNATHYQAIHHQAIDQVVLINLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLHTDKWLACYPSLCYQRQGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSHKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWCAVQGQLAEIRHHYCDAFMDGK
                     AGERAAMVIMEALG"
     gene            37120..37521
                     /locus_tag="OHN28_001464"
     CDS             37120..37521
                     /locus_tag="OHN28_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296882.1"
                     /translation="MLQKIGYMEEDNIIEKKPWRGIPPEYTDRWREIYNNAAPEEGMH
                     LSDACPVCGDTELYRYYSLLKTQPRLLGGIMYKGPGSYWEWCGSCRSYEHMSGYVPIS
                     WVVELSGIDHSKLTAIPDMIDIARHKNHYFS"
BASE COUNT         8656 a        10304 c         9683 g         9213 t
ORIGIN      
        1 taaatacccc aataattaat tttaaggttt tccgtgttct cttctgcatt atgaaaatgc
       61 aaaataaggc acaaaacaag cgcacagacg cattaaacac acatcaccta tacatcccta
      121 taccaagcaa tgaaaacagc gttagaatgc gtcctgttgc gttctggaga ttttcagaaa
      181 tcctctctaa gccatttcaa ctcactaagg agttttcttt cttcgttctc tctctgttta
      241 cgctctagcc aggcttttcc ttctggcgta gacaggaact tacgagcgtg aatcttgcgg
      301 ttattacgtt tgatagctgc aatgtttttc atcgtgtgcc tctgtattca tatgaaaatt
      361 aacaagttac atactacaca gggagactac gcataaaaca caatacaagt aaagatacat
      421 tttcaagaca ataggagcca taaagcgcct ttgatggtta taccaaaaaa taagcgccgt
      481 aggcgtatcc actaaaaagg gctttttcac aaaagctatg aagtcttagc agttcttctc
      541 ttatcaggca cccgttgtac tccggtggac gaaaccgtgc tagagcttga ttctttcccg
      601 atcaagttag gttttgaaga ggtgggcttt gatccactat tgcacttaaa actcttccca
      661 tcatttagcc tgtttatcct ctgcaacgtc tcacgacgtg tagcatcatt ggacaccgcg
      721 ccaggtgtgg aacatcttgc taagagtttt ggtagaaggt ttagagacgg tggcacgtct
      781 tccagcttca aaggaacttg ttacgaggtt gagcctggga actactcgcc catacgtgtt
      841 aaactaccaa agcccataac aacgtatagg cgtttgtaag tgataacaga agatacaatt
      901 tcttagagaa gttaagctaa gttattgatt ttcttgatgg tgccgataat aggagtcgaa
      961 cctacgacct tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa
     1021 ggacatgttc acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc
     1081 aatggccttg tgaatcaaat ggctactttt gcatcacccg gttttattta cgcacgaatg
     1141 gtgtaatcac caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggccg
     1201 cgcgcgtgga gtttttgtgt gcttaccgcc acttccgccc ccagtccaaa ctggccgccg
     1261 tcggtaaaac gcgtagaggc gttaacgtaa acagcggagg aatccacttc gttaacaaaa
     1321 cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg agtgttgcgt gccgtgttca
     1381 cgaatatggg caatggcgtc atccagatcg ctaacgattt tgacgttcaa atctaatgac
     1441 agaaactcat cgtcatactc ttcggcttta acagccacca ccttcgcggg gcctgcctgc
     1501 aactgcgcca gcgcggctgt atcagcgtgt aacgtcacgc cgctttccgc catttgtttg
     1561 cttaatgcgg gcaggaagct atcagcgatg tttttattca ccagcaacgt ttctaccgta
     1621 ttacacgtgc tcggacgctg agttttcgcg ttgacaatta ctttcagggc ttcagcgatc
     1681 tctgcgcttt catcaacgta aatatggcat acgcctatac cgcctgtgat caccgggatt
     1741 gtcgactgct cgcggcacag tttatgcaaa ccagccccgc cgcgcgggat cagcatgtcg
     1801 atgtatttat ccatacgcag catttcactg accagcgcac ggtcaggatt atcaatcgcc
     1861 tgcacggcac ccgccggtaa gccacaagag ttcagggcgt cctgaatcac cgccaccgtt
     1921 gcagcgttag tgcgacacgt ttctttgccg ccgcgcagga tcactgcgtt acctgttttc
     1981 aggcacagcg aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat cacgccaata
     2041 acccccagcg gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac gccgccatcg
     2101 attacctgcc ccaccggatc ggcgaggttg cacacctggc gcacatcatc ggcaatgcct
     2161 ttcagccgtg cgggcgtcag tgtcagacgg tcaagcatcg cttcgctaag gccattggca
     2221 cgcgcgtctg caacatcctg cgcgttagcg ttgaggatac tttcgctttg tgcttccagt
     2281 tcatcggcga ttttttccag cacacgattt ttttcgcggc tggagagttg cgctaattta
     2341 tacgaggctt gcttcgcggc aatgcccatt tgttccagca tcggccagct ccttaacggg
     2401 taatcatgtc atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcaa
     2461 tttcttgcga gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc
     2521 cgtgggcgat atcgcgacct tcgaggttgc aaatacggat gacttcacca cgcgagaaat
     2581 tgccagtcac gcttttaatg cctttcggca acagggagct gccgcgttcc agaatggcgg
     2641 cagttgcccc ttcatctacc gtgatttcac ccgcaggcgg cgcaccgaaa atccagcgtt
     2701 tacggttttc aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca
     2761 tcacatcacc aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac
     2821 ggcaagccac gtcagcggcc tgcaatttgg tgctcatacc gccagttccg aggcctgaaa
     2881 cgctatcacc ggcaatcgcg cgcagtgcgt catcaatgcc gtacacatct ttaatcagtt
     2941 ctgcctgcgg attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagcagca
     3001 acagtttatc ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg ttatcaccga
     3061 ccttaatctc tgccgtagcg acagcatcgt tctcattaat taccggaacg atattgttat
     3121 cgagcaacgc acgcagggta tcgcgggcgt tcaggaagcg ttcacgatct tccatatcag
     3181 cacgggtcag caacatctgc ccgacatgaa tgccgtaaat tgaaaacagc tgttcccaca
     3241 gttgaatcag tcgactctgc cctaccgccg ccagcagctg ttttgaagcg atagtcgctg
     3301 gcaattccgg gtaccccagg tgctcacgtc cggcggcaat cgcacccgac gtcacaataa
     3361 caatccgatg cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg
     3421 cacggttcag gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg
     3481 tctggctgtc actcatgatt ctctgccatt caattttagg aaaaaatgat atcaaacgaa
     3541 cgttttagca ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt
     3601 gcgcgggatc agcaagcgta gcggcagttg tttacgcttt tattacagat ttaataaatt
     3661 accacatttt aagaatatta ttaatctgta atatatcttt aacaatctca ggttaaaaac
     3721 tttcctgttt tcaacgggac tctcccgctg aatattcgcg cgttaattaa aatcaggaat
     3781 gaaaatgaaa aagagcactc tggcattagt ggtgatgggc attgtggcat ctgtatccgt
     3841 acaggccgca gaaatatata acaaagacgg taataaactg gatgtctatg gcaaagttaa
     3901 agccatgcat tatatgagtg ataatgacag taaagatggc gaccagagtt atatccgttt
     3961 tggttttaaa ggcgaaacac aaattaacga tcaactgact ggttatggtc gttgggaagc
     4021 tgagtttgcc ggtaataaag cggagagtga tactgcacag caaaaaacgc gtctcgcttt
     4081 tgccgggttg aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggcgcgtt
     4141 gtatgacgtg gaagcctgga ccgatatgtt cccggaattt ggtggcgact cctcggcgca
     4201 gaccgacaac tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt
     4261 cttcggcgtt atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg
     4321 cgacgttaaa aagcaaaacg gcgatggctt cggcacgtca ttgacatatg actttggtgg
     4381 cagcgatttc gccatcagtg gagcctatac caactctgat cgcaccaacg agcagaacct
     4441 gcaaagccgt ggcacaggca agcgtgcaga agcatgggca acaggtctga aatacgatgc
     4501 caataatatt tatctggcaa ctttctattc tgaaacacgt aaaatgacgc caatctctgg
     4561 cggctttgcc aataagacgc agaactttga agcggtcgct caataccagt ttgactttgg
     4621 tctgcgtcca tcgctgggtt atgtcttatc gaaagggaaa gatattgagg gcatcggtga
     4681 tgaagatctg gtcaattatg tcgatgttgg tgctacgtat tatttcaaca aaaatatgtc
     4741 agcgtttgtt gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa
     4801 tgatgatatt gtcgcggttg gcatgaccta tcagttttaa tggatattgc cggatgtgat
     4861 gcatccggca catttcattc acgccgttaa cttcaccggt tcatcacgaa aatcatctgc
     4921 cggttccagc ttcagattca gcgtcgccag cagctcacgc agcttctcgt gaaattcacg
     4981 cagggtgtgc tccagtcgtt caaccacttc ggcatctttt atctgaacac tcgtccagtc
     5041 gcctgcttta tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc
     5101 cagttccatc caccagcccc agaactcacg cacttccggt gccggtttca cgttgacgca
     5161 tacagccaga caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg
     5221 gcctagtgcg gtaaattttt tgactaatct gctcttcggg tgtccactcg gtaacgtcat
     5281 tgcaatctcc tgttgtgatg caactgtttt accaaattgg taaaatttag caaattttta
     5341 acacaaacgt ttttcgatcc agtctgtgat ttcctgaagg cctttgtcaa aattccgata
     5401 caccgggtta aatgggatct ctaataattt accgtcagca gatgacgacg tgattaagcg
     5461 tgagtcctct tccgggctga acggatcgtt cttccagtag cctgataaca ttggcgttgg
     5521 gcagcgacgt cccagcaacc cttgcacttt taatgaatag cgattcagct ccacgcgcaa
     5581 cgcttcgtca gaagcatcat gcatccccaa acgactcgcc agaacgtcaa gatacatttc
     5641 cggcacctgt tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca
     5701 ggcaaccgct ttcagacacg gcgattcaag gtatgccaga cgtacggcaa cgttagcgcc
     5761 gaaacggaaa ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcaacgc
     5821 ctttaaaacg tgctgatgca acaggctgga gtcctgggtg agcttccatt ttgaagaaaa
     5881 gcccaccgac ggcatatcaa tagtcagcat cgcaatgccg cgcagcgcaa aataacgttc
     5941 atacaggctg taatagtctg tctgcatcgc atccagacca ccacacatta atactgtcgg
     6001 gaacgggcca tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac
     6061 ggtaaactcc atctgccgca tcgtgcccgg cagacgctga gcggcctctt cataggcgcg
     6121 gtttgacaaa gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcagc
     6181 aatgttgtac aacgtagccg catgcagcca gtgtcgaccg ctgagttgcg gatcttcttc
     6241 cgcacaggct ttttgttgcc acaccatcgc ctgggtagcc cactcataaa tccagttgcc
     6301 gccgcggtag ccaatcaccg tatcgtataa atcatcgtcg gtacgttcgg catcgctcat
     6361 cacaatacgc gcctggactt cgaggatttc acgggggtca atgccgcgcc agatccacat
     6421 cagacggtta atcatgcgat accagtgagg gatggtttta ccatcaaggg ccgactgcac
     6481 aggcagttgt gcgccgtgac taaagcggcg gactagcgtc gaggtttcag gatgtttaaa
     6541 gcggggtttg aacagggttt cgctcaggtt tgcctgtgtc atagatgcag cctccagaat
     6601 acttactgga aactattgta acccgcctga agttaaaaag aacaacgccc ggcagtgcca
     6661 ggcgttgaaa agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg
     6721 gctgttcgat ccaggtattt tgcgggatat caacaacata atcatcaacc agcggacgac
     6781 cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa
     6841 ccgcagtacc accggtatct accaggtcat caataacgat gaagccttcg ccatcgcctt
     6901 ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa
     6961 cggtatcgac atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc
     7021 cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt
     7081 tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg
     7141 tcccagcctg tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat
     7201 tatagagatc tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac
     7261 tgcctctccc tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg
     7321 tgttgacaac attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca
     7381 aggagactta acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt
     7441 gccaaaatct gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt
     7501 gttggttggg caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcctg
     7561 attcgtaaac ctgctaccgc aggtatggaa aatcgtaaac cggtcgtctt gcaggcccac
     7621 ctcgatatgg tgccgcaaaa aaataacgac accgtgcatg acttcactaa agatcctatc
     7681 cagccttata ttgatggcga atgggttaaa gcgcgcggca ccacgctggg tgcggataac
     7741 ggcattggta tggcctccgc actggcggtt ctggctgacg aaaacgtggt tcacggcccg
     7801 ctggaagtgc tgctgaccat gactgaagaa gccggtatgg acggtgcgtt cggcttacag
     7861 agcaactggt tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc
     7921 tacatgggat gtgcgggggg tatcgacttc acctccaacc tgcatttaga tcgtgaagcg
     7981 gttccagctg gttttgaaac cttcaattta accttaaaag gtctgaaagg cggtcactcc
     8041 ggcggtgaaa tccacgttgg cctgggtaat gccaacaaac tgctggtgcg cttcctggcg
     8101 ggtcatgcag aagaactgga cctgcgtctt attgatttca acggcggcac actgcgtaac
     8161 gccatcccgc gtgaagcctt tgcgaccatt gctgtcgcag ctgataaagt cgacgccctg
     8221 aaatctctgg tggataccta tcaggagatc ctgaaaaacg aactggcaga aaaagagaag
     8281 aatctggcat tgctgctgga ctccgtagcg aacgacaaag ctgcattaac ggcgacatct
     8341 cgcgatacct ttattcgtct gctgaacgcc accccgaacg gagttatccg taattccgat
     8401 gtagccaaag gcgtggttga aacctccctg aacgtcggtg tggtgaccat gaccgacaat
     8461 aacgtagaaa ttcactgcct gatccgttct ctgatcgaca gcggtaaaga ctacgtagtg
     8521 agcatgctgg attcgctggg taaactggca ggtgcgaaaa ccgaagcaaa aggcgcatat
     8581 cctggctggc agccggacgc taactctccg gtaatgcatc tggtacgtga aacctatcag
     8641 cgtctgttca acaagacgcc gaacatccag attatccacg cgggcctgga atgtggtctg
     8701 ttcaaaaaac cgtatccgga aatggacatg gtgtctatcg ggccaaccat caccggtcca
     8761 cactccccgg atgagcaagt tcatatcgaa agcgttggcc attactggac actgctgact
     8821 gaactgctga aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa
     8881 cgccttccgg cagtttcatc cttcattatc cttcgataaa agccatccct gtgaacgttc
     8941 gtcgcgggtt gccacgttca atctggtgat ggaacattcg ccgctgcgat ttcagcgccg
     9001 cgctattttc ctgttgctgt tgttccagct tccaggcaat cagtaatcgc gccagccgtt
     9061 tgttagcatg ctgactgcgc tctgactgaa ccttcacgct aataccggat gccagatgcg
     9121 tggcgcgtac cgccgagtcg gttttattga catgttgacc gcccggcccc gacgaacgca
     9181 gcgtctcata acggattgca tccgattgtt cctgctcatc agcggtaaaa cgcccaatgc
     9241 ccagaaacca gtttttgcgc ccatgatgag gccgatacgg actcggacaa atccactgaa
     9301 tagtgccgca ccagctttcg cttagagccc atgcgttatc gccatccaga gaaatcagcg
     9361 ccgaacgcag tgtgtcagag tagcgacccg tttctgtttc cagcaccgtt accgcgacgt
     9421 cctgtcgggt agcttcttta atcagcctgt ccagtgcttt tttcactgcg agacaacatt
     9481 cttccggccc ctgagcagag gagagttgta gcaagatcat ccttttttcc ctccactgtt
     9541 tttgagcgtc agcaccgggc gcagtcgcgc gacaggaata attaacccag ccagcaccag
     9601 acattgcacc acgctttcag ccgatttata ggcttgtggc gcttcttcaa agatgagttg
     9661 tttatcgcga caaattaccc gactgccaag ttcagtccgc gagagttgcg tcgccgtgta
     9721 tttcgctgcc agacgccctt tacactcggt gcgcccccat ttacgccccg ccccatgcgc
     9781 cagcgagtgg agcgtttttt cgttcgcaac aggtttaacc agccaggagt aatcgccgcg
     9841 tgaaccggga ataatcacca gaccgttgtc atccggcgtg gcgcctttac gatgcaacca
     9901 gccctgttga tcgccgattt ggcacgcgct aacaaagtta tgcgccacat ccagaaccgg
     9961 acttccggtg gccttaactt gttgcattat gcgcaaagcg atcagctggc gattaatacg
    10021 cgcaaacgcc agcgcatcgt catgttccgc aatataccgt agcgcgtcgt cactgccttc
    10081 aggcaaacca tgatgcgaaa acgaggcaat atgccgctgt aaaatagact gccccaatcc
    10141 cctcgagccg ctatgaacca gtagttgcag atgctgcgca tccagaccag cccgtgcaaa
    10201 cagttccgcg tcgataattt gatcaatctg ttgcagttct gcgaagtggt taccgccacc
    10261 gatggagcca agcgagttgt gccacggatg ctgtgcaaat gctgacggca ggttttcctc
    10321 cagccagctt tcttcagcaa cgtcatccag atctgataat cgcttttcaa acttatcggc
    10381 gttgtatttg cgagcgagaa tatctgtttg ccatagcgcc atgccgcagc cgatatcatt
    10441 gccgaccagt gccgggtaaa aacgaccaac agagaagaac gctgcgccaa tcgggtagcc
    10501 gcgtccggga tgtaaatctg gcatccccac aacgcgctgc atgttgggta aatttgccgt
    10561 ggtgtgtaat tgttggatcg ctaaactctc gatccacagg tcatcagatg cgatggtaaa
    10621 taccgcatcg gataagggac gaatatattt gcccatgtac cattccattt atgaataaaa
    10681 atcaggaaat ggcagacgaa tccagggcaa gaaaaaagaa ttaatcctgg aaaggtctgc
    10741 aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt ggaataagaa
    10801 aacgcgcaca tactagacgg gcgttatttt cattgcaagc tggatttaat gttgcggttt
    10861 atatcgcata taaaaattag taaaccacgt tccccggcat tcaacgcgct gctgcttaac
    10921 tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgctggcgt ccaccgtaag
    10981 ttcggattca gacttaatca atggttttaa caaagcgctg gcaactccgc gtcgggtgta
    11041 ttccggttca acaaataaca tatcgatata atgtccaatg cgggtaataa aaccaaccgg
    11101 ttgtgcatta ataactgcaa cccgcacttg tgatttcgcg agtttctcct tccagcgaga
    11161 ttcgtcaatc tgcgcccagg cggcaatttg ctgtggagaa tagtgctgac tcgccgtcat
    11221 cgtaacagct cgaaggaaaa tagcgcatag ttgctgaaaa tcaccaggct gatagtttct
    11281 tatttgtatg ttattcataa tcccagcacc agttgtcttt ccatttgcgg gtcaagcaac
    11341 gtcacatgca gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta
    11401 cgcgcggtgg cgattagatc agatttattc agccgcggcc agacgtgttc ctgggtggtt
    11461 tgctgaaaat cgtcgaactt taatttcacc ccctggcggg caatcagcaa atcaggtttt
    11521 acctttgcca aacgacgctc cagttcaggg taaagccgct cgatgatcgc ttcacattca
    11581 gaccagtgat gaatatcttc cgccatcgta cgttctacac cgacggattt tcgcaacctt
    11641 tcgctgttaa cgtcgcgttc gtcaatgccc tgactacgct cccacaaaat gcggccaaat
    11701 ttgccaaagc gtttaagcag catcaccaga tcacactttt gcacatcacc gcaggtccgc
    11761 agccccatcg cttccagttt tgccgccgag actttgccga cgccgggaat ttttgccagc
    11821 ggtaaggttt gtaaaaatgt cggaacttct gccggcgtaa tcacaaactg gccgttgggt
    11881 ttattcatgt cggaggcgat tttggcgaga aactttactg gtgccacgcc cgcagacgcc
    11941 gtcagttgca gctcgttgaa gatcgtctgg cggatttcct gggcgatgag ggtcgcagaa
    12001 ccgtggcaat ggacgctatc ggtgacatcg agataggctt catccagtga taacggttca
    12061 atgcgcgagg tatagcgcga gaaaatttcg cggatatgat ttgaggcttc tttgtaggcg
    12121 tcaaagcgcc ctggaagcaa ggtgagatgc gggcataatt tgagcgccat ccctgtcggc
    12181 atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccgt
    12241 cgttcgcggc tgccgccaat agcaataggg atatcgcgca gggcgggatt gtcgcgcatc
    12301 tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat tgctcacctc
    12361 tcaatactgg taaagtatac agtgatttca gggtttgaga aatgcgtaaa gattcagcat
    12421 ttaacgcgtt cgctgcgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac
    12481 cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag
    12541 caccataatc tcaatgcgcc ggttgcccgc gctttgcgga tttttggcat ccagcagcat
    12601 ctggtccgcc attgcactta cctgcatcac tttatcttcc ggcattccgg cctcttccag
    12661 cacccgacga gctgaaagcg cgcgatcgcc cgaaaggttc cagttgttgt agatattgtt
    12721 tttgtaggcc atcgcatcgg tatgcccggt aataataatt ttgttatcga gcgagtcgaa
    12781 cactggcgca agctccacca gcaaagtttt aaagaacggc ataatcttcg cgctgccgcg
    12841 ttcgaacata ttgcggttct ggtcgtcttt aatcagcacg cgtaatccct ggggaacaat
    12901 ctccatctcc agattcgctt ccatatgcgc atcacgggcg atggtattga tactggttgc
    12961 cagctcgcca agctcggtgg cggatttctt cttcagcagc gccgttttct cgttgacgtc
    13021 gcgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg gcggatgaga
    13081 cgggctgatt ttatttaacg gcgacaaccc accaccgtta aaaatcgact gcccgtgcag
    13141 cgcggcgata atgctttcac gctcggattt actgacgctg ttgacaatcc acagcgtcat
    13201 aaatagcgcc atcattgcca aagtaaagtc ggcgaacgcc accttccacg ccccgccgtg
    13261 atgcccggca tgattctttt tgatctgccg ccggacgatg gtggtttttg ccccacgttc
    13321 gcggtggttg cccgtctttc tcatgcttat tcttgctcca gcatcgcatt cacccacgcg
    13381 tcgaggttgg cgaaggtcgg tttggacgcc agatgcagca gcttacgccc ggcgtccacc
    13441 gccagcagcg tcggtttgcc gcctgcctgc gccaccagca cagtgcgcac gcattccagc
    13501 aaagaatgtt ccgcccgcgc ctgctgttcc atggcgttgg ctagcgggtc catcaggcag
    13561 tagcagataa acacgcctaa aaacgtcccc accagagccg ccgccacttt caggccaatc
    13621 agagcgatag atccatcaat cgactgcatg gtgatgatga tgcccagcac cgccgcgcaa
    13681 ataccaaatc ccggcatcgc ttcggcggta cgttgcagcg agcgcgacgg cgttaacagg
    13741 gactcttccg cggtgtccag ttcctgatcg agaatgcctt ccagctcatg ggcgtcgatt
    13801 ttacccatcg ccatcaggcg aaagttatca gcaataaagg tcaccagccg cttctgggtt
    13861 aacaccagcg ggtatttctg gaaaatggtg ctctcttccg gctgctcaat atgctgatcc
    13921 aacatccgca gaccgccgtt ttgcaccatt tcgagcaact catacaggca cattaatagc
    13981 tgacgctgaa actccggccc gagctgtttt ttactgatca cgcctttaat ttgatgggca
    14041 atctctttca gcacatgttt cgggttacca ataatcatcg ccccgaaacc ggcacccaaa
    14101 ataatgatca tttccgccgg ttgccagata gccactaatt gcccgcccgc ttcaaaataa
    14161 ccgccaaata cgcagccgag caccaccagt aaacctaata ttttttgcat gatttcttca
    14221 ctttaattcg gatagacaaa atcacgaatt ttctgcgcga tctgtttatt cagttgacaa
    14281 attcgggcct cggttagccc taataccagc gcaatctctt tgagattcat ctcatgctgg
    14341 taatacatgg agaggatcag ttgctctttt tcgcttaatt gtgaaagcgc gtgttgcaac
    14401 atttgctgag tgacaaactg atcttccagt tcccggccag caagcggaat gcctggccct
    14461 tcgccgttca gcagttcgtc caggctggcg agcgtttccg ccccttccag ttgctgatat
    14521 tcgagataat cttcatgcga gatcccttcc tcagccagct cctgccactc cggttcgtgc
    14581 cccagttttt tacgcgtttc gcgaatcagg tctttcatct gatgatattt ctgtcgcagc
    14641 tggcgcggtc gccagtcgag agcgcgcagc tcatccagga tcgcaccgcg aatacggtgc
    14701 acggcatagc ccgcaaaacc ttcgtccggc agcccatagc gacgaatggc gttcaacagc
    14761 cccattaacg ccgtttgttc catatcctga cggtcaataa tgcaattaca ctgcggggca
    14821 agttgccgca ccactttacg caccaacggt aaataggcct gcaagtagtg gctctcctgc
    14881 tgcggagtta ataccagcgt ggctgcaaat tcttctgact cgaatacatc ctgtagcatg
    14941 tttatatccg cctggtgatt actggaagac cacttttttc accagcaaat ccttatacgg
    15001 tgcggtcatt ttgcgacgct gaagatcttt ctttaacgcg tcagaaatca gtttgcggat
    15061 ggcagatatt ttcatgccgc gcaaatcttc aaatttcatt tccgtcaggc attcaaccgc
    15121 aatactttga tacagcggtt cctgatgctt tattttttcc gcctctttgt cactggcgac
    15181 caccatcact aattccgccg acaaataatg atccgcgccg ttgttgtcat gcaaagtgac
    15241 aatggtttcc ggcagcgtca caaatacgct cttgctctca tccagagctt ccaccgattc
    15301 cgtttgcgcg gcggtagtcg gttttccttt tccggcataa tgatgccaga cactccatcc
    15361 tgctccagcg ccgaccacca acgccagaac gacgctgacc acgcctgcca ccacgatttt
    15421 cttcatattg cctttacact ttgatcaaaa cggattcgtt ctgttgaaat tgaggcgcat
    15481 catcaagctg taaggcggca ctcacctctt cctgctgatg gcccgactgt tgttgctgct
    15541 gcgactgccc ttcggaggaa acctgtacgt tcacctccat aaaattttgc gccgtcagat
    15601 gctggcggag atcgtcgcta aactgctgca atgcgcggca gacttcgctt tgattcgcac
    15661 cgatatggac cgtcagtttg ccgttatcca actgtacggc gatctcaagc ttgccaagcg
    15721 acggtggatc gagacgaatg gtggagatct gctgttgttt gttgagctga aagtggatct
    15781 gatctttcaa cagcgtcgtc agtttttcgc ccagttcgtc catcgaaagc gtggctggtt
    15841 tggcgacaat ctcctgatga tcgctgacca actctggcgt cttaacctgc aacaccgcac
    15901 gcgcaataac cggttcgctg tccggcactt tttccgtttt acgctcgacc tgcgggcgcg
    15961 cggtcacacg ggttagttca ggtttcgggc gagctggcgg tgttgagacg cgcggctgcg
    16021 tcggcgcaat ggcatgtaaa tcttcactgg cgtaagtcgc cagtctggcc tgctgttcgg
    16081 gtttcgcctg cggcaactga ccgatcagtt cctgcaactg cggcggcaac gacgtgagtt
    16141 cctgctgctg cgtcgggcgt tgcggcgcgt tttgcaccac cgctttcgtc aattgctgga
    16201 taaccggcga tgttgcgacg tttcgcggct gcggcgtgtc ctgatgcggc gcggcaggtt
    16261 gcggaagtaa cagcgccatt agcgcctgca tcgccaccgg atcgttctcc tgcgggcgcg
    16321 tcgcctgttg ctgactggtt ttagcgctat gaacgcgctc cgccaccgcc gcgaccacca
    16381 ttttcggcag ggtaaacacg ggcgcgtttt caccgctcac cggcggtaaa atatccgcct
    16441 tcaacgaagc ggtttccgca agcgttccga gcgtggcaag caatgccgga ttcatgactt
    16501 ctccgaaagt aaattaacaa ttgagtaggc cagtacgcct tcctgatgcc gctgatgctg
    16561 ttccatacgc gctttcaaca cttctgcgcg ctgctcgcgc cgtttaatca gggcattaaa
    16621 cgcgccgtac agttgtgcct tcaccgcatc caactgcgcc gcgtcggttt ccgccgccgc
    16681 gtaatcgtga aactggcgca tcaactgctg atacagtgcc ggaaggcgtc gccagcgttg
    16741 cttgtcgagg gcgtcattca tcgcttcgac cagtgcaaaa agttgcctgc gttgtttttc
    16801 catcgtctta acccagcttg cccgaaagcc cttcccagcc ttcgcgcagg ttgcttaaga
    16861 tcagcatcac ttcatcgatt ttctctgccg acagttcgcc gctggcttca tacaagcgat
    16921 acacacagtg gtcatagagg cgcgaaaggt tgaccaccag ctcgccgccg gtttcaaatt
    16981 ccagcgaact ggtgagcgca ttgagaatgt cgatgcattt attgatgctc tgggctttct
    17041 tctcgaaacg ccgcccttca atgtggcttc tggcgcgttc cagctcgtcc atcaggccag
    17101 aaaacagcac cagcaccagt tcaagaggcg atgccgccgc ggtacgcgcc gcgagatcga
    17161 tctctttgta ttgcgaataa ccgtcctgta cttggtacat ctttaaggat attccttctg
    17221 aattacgcaa atgccgccat actggctttc atggtgtaca cctcaaccaa cgtattggtg
    17281 tactcctcga gataacggtc gtagttagcg ttataggtat cggtaagctg atcgccttcg
    17341 tcctgaattt tgctcatctg atcatcaatg ttctgctggc gcagggtgat gatgccgttg
    17401 ctggagtccg tgtaggtgtt aatcaggtcg tccatctgcg ccaccatgct gttgtcgcca
    17461 acgaaaatag aggtcaggcc gtcgggatcg ctcttcatcg cgtcgttgaa ctggtcggag
    17521 tcaatctgta agtggccgtg agaatcgagc gtgatgccat agtcgacaat cgacacgccg
    17581 ttgtagctgg catgggcgat gtcatcgagc tggtttgcca gtgagctgag tccagcatcc
    17641 cccgcaaaga ccccggcgct actactgtca tcaccgtggg tggttagcga atcaaccgta
    17701 tcaatcaagg tgttataggc atcgacaaag gtctgtactt tctcctggct ggcgctggaa
    17761 tcttcactga tgttaaaggt ggtgagatcg ctgtcagaat cgctgacttc ggtaaaagtc
    17821 atggtgacac cggggatcac atcatcaaac gtgttgctgc tgttggtgat agccggtccg
    17881 ctggcgctac cgagatgaat aatcgcgtcc tgagcggagg aaacatccgt ggcaacgggc
    17941 gcactggtgc tatcgttgct ggcatcatgc cccgttacgc tcaccgaaaa cgcgctctgc
    18001 gccccggtgc tatcggaggt gagcataatc gtggtggtgc cgtcggtttt taccagtgcc
    18061 gccgacacgc ccggattgtc atcggagtcg ttaatcgcat tcaccagctc gctggcgtcg
    18121 ataaaaccgt caccgtcacc gttctgatct gccgccgaca gatcgatatc catagtgctg
    18181 tcgcccatcg tcagttcaaa agtaccggta gcggaaaagg cgtcgtcgcc catactgaac
    18241 gtggtttgct gcccctgcgc cagttgctca acgaaaaacg agtagcttcc cgcctgcgcc
    18301 tgggaattgg cggaaacggt cgccgagtca ttattgctgg tggcggcaaa agtcaccggg
    18361 ccgtcggtat cgctgttcag cgcgtcaacg gcgctctgga aatcgctcaa cgccgagctg
    18421 agcgagtcca ggccgctgct ttcagcatcc agctcactct gcttctcctg caaattcgtc
    18481 gcctgagtag cgacatccgc atacgcgatt tcttgcgcaa tggttcttgg gttgatcatt
    18541 gatgactccg gataacaatg tcaggttatt ggcaaaaggc gtgccaatat tttttatgtg
    18601 aataatcaat agattgaaaa acaggcggaa atgacgtttc cggtctggcg gaaaaaagcc
    18661 tgccgcagaa agcacaacac cgcccacagg cggtgttgtt cgtggccgga taaggcgttt
    18721 acgccgcatc cggctatcgg tgcacagtgc ctgatgcaac gctgacgcgt cttatcaggc
    18781 ctacgagtcc gcgcctgttt ttatgcgtgt gcccatttgt aggccggata aggcgttcac
    18841 gccgcatccg gcaatcggtg cacaacgcct gatgcgacgc tgacgcgtct tatcaggcct
    18901 acgagtccgc acatattttt atgcttgtgc ccgtttgtcg gcccgaactt actgcagcag
    18961 gctggaaacc atgctggaca tgctgttgga ctgtttcagc atggtgatac cggtctgcat
    19021 cagcacctgt tgtttggtca tattggacgc ttccgtcgcg taatcggtat ccatgatgtt
    19081 gccgatagcc acttcggtgt tgtcctgcat gctggtcagg ttgttggcgg tgtcattcag
    19141 acggttgata gatgcaccca acttggactg aatctgagaa acgtcatcca tcgcggtaga
    19201 aatgctggtg atcatctggt tagccgagcc agacgcagtc agttcagtac cgccagaaac
    19261 gcctgcggtg ccagtggtgt cagcctgatc cgcactaaag gagttgctga tagcagaaag
    19321 gtcagtcacc agcgtgttca gttggcttga aatgttcacg gtcatggtgt cggaagattc
    19381 cgcgcccacc tggaaagtta ccgcgctctg gaacagaccg tcagtaccgg tgttgctggt
    19441 gccggaaaca ccgaacaggt tggtgccgcc gtaagtagtg ttttgcagca tgtcagacat
    19501 ctgctggccc agttcgtcgt attcgtcctg catcgcctgc aggtcgtcgt cgctgtaggt
    19561 gccgttcgct gcctgcgttg acaggtcttt catacgcccc agcacgtcag acatttcgtc
    19621 gaacatgctg tcagcggttt gcagcatcgc ggtagcatca ttgatgttgc tcagcgccac
    19681 acccataccg ctggactgtg ccgtcagacg gttagcaatt tgtttgcctg cggcgtcatc
    19741 ggcagaagag ttaatccggt tacctgtcgc caggcgttcc atagaagtag acagagaaga
    19801 gctgcttttg ctgattgcat taacggcggc cattgaggcg ttattggtgt taatagataa
    19861 catgatactg ctccttggga ttaatcgtgt tcgtttcgat accctgtaaa aacgacacct
    19921 cgggagcaaa cttaaaatcg ccgttaaaat tttttcgcca cgtattatcc gatgccactt
    19981 ttagctaaaa aaagcagagt ttaaattcag ataaaaagat tattagcaat atctgccaca
    20041 tggccaggta tcaaaatatg tctttatcaa atgcttgata tagttaagat tgtcatcaat
    20101 taacgcgttt tcttctcaat gtactgatgc caaaatttac attgcgttgc aaatagtaca
    20161 cgtttggcta ttgtacctgt cctgtcattt cgttacattt ccacgccata tagtgattaa
    20221 gaataatcct attaccataa aatattaatt atcacgcaga aaaatcatct tctcagggac
    20281 gtataagact gtgctttatg caaagcacta tcaggaaaca tttatccctt gctaaggtaa
    20341 actcaactat gagtattatt atcaacaact ggcggatgga cccgtcgctt aatgccttaa
    20401 tacattgcga aacgggtgaa acacgtcgtc tcggcgaata ccattttatt ttgctggaaa
    20461 cattagcaaa aaatgccgat gtggttttat cgcgatctta tttatgtgcc gaagtatgga
    20521 agaaccgcat agtcggcggt aacagcctgc cgacggcgat ccatgcgttg cgcgttgcta
    20581 tcgatgatga tggcaaacaa cagaacatta ttaaaacaat tccgaagaaa gggtacctgt
    20641 gcaataaaga gtatgtgtct tttccggagt catcgcctgc ggaagcgctg attattaccg
    20701 accagataca gaaaacaacg ccagaagaga tatcttcgac aacaccgcct gtacctgtta
    20761 gaaaaaaaca taaaggactg atggggctgg cgttggccgc ggctgttata ttggttggct
    20821 caaccgtggg gtattcacac ttgaaaagta cacctgacgc cccgcaactg gtaaaagagt
    20881 caattaacag tccaagaata aaaatatttc atcttagttc tggaaaggaa aataattccg
    20941 taccgttact gtcacaaatc ctcgcaccag gaaaagacaa actcgataat ttattgtcag
    21001 cgcataatat gacaatgaca acctattata aatatgttcg taaccgactg gaaagcgata
    21061 ttgttctgcg taaccagtgc aacggcagct ggcaattaac ctttaatgtg gaaggttggc
    21121 agaacagcga tattaatagc gcgatgtatc agaatttaga gaagttgtta aatactgtgc
    21181 agaaatgctg acaagtgcca atatatgccg gatgcgacgc taaacgcgtc ttatccggcc
    21241 aaccgataat tatcccaata aattcctcac cgtctggctg gaaattcgcg cctgaccgtt
    21301 caccgcctgc aataaaatct gatagttatg attggcgcta tccagcacgc cgccgaggtt
    21361 ctccgatgcc tgcaccgcgt tttccgtttg cgggaagcgt gacatgccgt cgatcaactg
    21421 gcgggctttt tcctgctgca ccgccatctg cgcgcgctgc tgattaatgc tttctaacgt
    21481 ctgggaaatc cccgccccac cctgttgcaa actttgcgct aaacgcacgg cctgcgacgg
    21541 ttcggcaaag gtttttagtg gcataaacgc cgacttatca ccatcggtgc gcacgcttaa
    21601 ggtgcgctcg atgtcaggcc actgtttttc ggtggtggag aaactgatgc cgtccgcttg
    21661 ctgatgtacc tgcacgccca cgcggcgcag ggcgttggtc aggcgcgtct ggtactgccc
    21721 ggcgctgtca tcctcagaca gcatcaccgc cgaaagctgg gtctgccgcc cgtcagagac
    21781 actgaacatg cgcgttcccg gcgtgggatt gtgtaccaga ttcgccagat ccggcgagtg
    21841 gaaggtcacc cgcgcttcgc cctgcaacac tggttgtaac tggcggtcca ccgcgccgcc
    21901 ggaaagcgcg gcgcgtttgt ccagcatctg catcagcgcc gtactctgcg cctgcccgcc
    21961 cttacgctgg ctgtgacgat agtcgagcaa ctgctgttcc agttgcccga gatagtgatc
    22021 cgcctgctgc aacgtggtga gctggtcgtt aagctgtacg ctgtaacgct gcgggcgagt
    22081 ggtaatcagc ggcgaggccg gatattcact cgccgtcgcc gggagttttt gccgcaccgc
    22141 ctgagtggac gcaacggttg acgacgatac cgcagcgcta tgtgcgccgc cggtggcgag
    22201 tgatgaagat gtaagtccga cctgcatact gttcctgaaa gagttagaga acgctgaaca
    22261 aattcaactc actcactttc gagtaagtct tgagggtagc ttccattgcc acttccagtc
    22321 cggtaaaggt gatcgacgcc ggaccgtaat cgagatcctg caatgaacca atcaattcat
    22381 cattagatgt cgagatatcg gtctgtgcat cgctcaacat cgacatggtg ttttgtgtct
    22441 cgccgagcga cgcaatcgag gcgttgaggt cgtccgaggc ggtgtccacc acatcgacgg
    22501 cgttctgaat atcgctctgc acctgcggat ctgccgggtc gacgtccggg ttttgcagtt
    22561 cctgcgacaa ggaattaagc gtgttaagca cgtctaaatt gctaccgaaa aaatcgctcg
    22621 ccgccacgtt ggtgtccact tccacaccgt tcgacacggt agtctggcga tggtcgctgt
    22681 tgccctgata gctatagctg tcggtgacgc catcgccatc atcgtctacc gcgacaatcg
    22741 gcggctgatc gttgatggtg ccgccaaaca cgtagtgccc gtcttcgtcc tgataattga
    22801 gcgccgccac cattgaatcg gtgagcgact caatatcctg gccgaggctt gccagcgaat
    22861 cggcggtatt ggtgccgttc gccgcttcaa gcaggtcgtc gcgtaccacc agcaggctgt
    22921 tgttgacgcc atcgagaatc gattcctgct ggcttaagcc cgccgacgcg gagtcgatat
    22981 tgctctgata ctgttcaatc gccgactgct ggcggtttaa ctgggtgatg cgcgtggcgg
    23041 cgattgggtc atccgacggc tgcaaaattt ccttgccggt tgccatctgc tcgaccacat
    23101 gcgccagaga gccggagaga ttattaaaac tttgcgtcat cgagacgtag gtttgttggg
    23161 tggttactcg cattttcgcg ctcccgctta gctgcacatt tccagcaccg aatcgaaaat
    23221 ctcggccccg gcggaaatga ctttcagatt agcttcataa atttgttgat aggtgatcag
    23281 gttcaccgct tcttcgtcca tgctgacgcc gctgacgcta ctctgctggt tttgcgctgc
    23341 ggaatagaca ttggacgcgg catcgacttc cgtctgattt tgctgactgt aaatgccgat
    23401 attgctgatg attgacgagc acgcctgccc gaccgtcaca ctgccgaggt tggctatctc
    23461 cagcggttcg gtggagatat tgatcagcgc ctgcaggttg tcgctgttgc cgctttcgtc
    23521 cggtgaactg gaaaacgcca gctcatcggc agtgatatcc gggttaacgg tcagtgggcc
    23581 atcggcattg ctggcgtcat aaataaacag tggctcgccg gggttaccgt tgagatcgta
    23641 gccctgcgcc agctggttgt tgaccgcatc ggcgaactgc gacgccatgc tgttgatggt
    23701 gtcggtcagc ggcgtcagca cgtcgttttg atagtcgaac agagcgccta acgaaccgcc
    23761 agtgtcggtg gtcatggtcg aggtggtgcc agcgaaagtc agcgacatgg tcggtgtgcc
    23821 gtcggcgttg gtttccagcg cgatggtcga gctttgctgc ccgctcacca gcggctgacc
    23881 gtttttcagg gtgacgttgt agttgccctg gtcgtcaata ttgacctgca cgtccatcat
    23941 tccgctcagt tcttccacca tctgatcgcg ggcgtcgtac agcgcggagg cgttatcgcc
    24001 gttggcttcg gcctgagcga tttgctggtt atagctggcg atgccgctgg tcagcgtatt
    24061 gatttgcgat accatcgcct gctgctgact gatgatttcc gtgctttgcg agtcgatgta
    24121 atccagcgtg ttgtcgatgc gcaacgacag cgccccggct tcgctgatca cctgctcgcg
    24181 cagggcggaa tcatcggggc tggtggtcgc ttcgttaagg gcagcgaaaa agttatcgaa
    24241 gccgccgctc agactgctgt tatcgtcgct cagtaccgct tccagttgtg tgagataccc
    24301 ttgttgggtg ctgtaatagc cgtaatcgct ggcggcatac catacctgat taacctgata
    24361 ctggttggag acgcggcgaa tactgtcgac ctgcacgccg ttaccagcgc tgttcggcga
    24421 accaccgctg gcaccgatgg tgctgatctc cgccacctgg cgggtgtagc ccgtggtcat
    24481 ggcgttagcg gtgttttgcg ccgtgacgtt cagctccacc tgcgccgttg atgcgccgct
    24541 gtagccgatg ttaatcatgt ccatgttggg tccttaccgg taaatcgtgc cggttaaaag
    24601 cggcgtgaag ttggggattc ttaagttccg taaaccgggt gccaaatgcc ggatgcggcg
    24661 taaacgcctt atccggccta catactacat acgggcgact cgtaggcctg ataagacgcg
    24721 acagcgtcgc atcaggcacc gaacgccgaa gcctcattta tgcctgggcg acaactgctt
    24781 cacaagcatc gccgcaatcc caatcccgtg ctgctgcgcc agggtgttac acaactcatc
    24841 gtcataaaac ccctgcatca tgcgcaccga atcgctgtta aacgggttat ctttcgaatc
    24901 aaacaactcg ttggttttac gcatctcttt cagcatgttg cgtaaaaaaa tcgcctcaaa
    24961 ctgctccgcg gcctgcttaa tatcattggc ctgaaccttc ggccccatta acgcatcgct
    25021 gccgtcaatc ccgccgctgg cgttcacttt catcagatca cctccaggtc ggcatccagc
    25081 gcgcccgcct catgcagcgc ctgtaaaatc gacataatgt cgtccggcgt tgcgcctaaa
    25141 ctgttcagcg cattgaccag cgttttcagg ctgctcgact ccggcaaact caccacgccc
    25201 gggcgcgcat gattcaccgc gatattgctc tgcggcgtta ccgccgtgcg cccgcccgca
    25261 aaagcatttg gctggctgac gttgctggtt tcgttaatcg agacggtcaa actgccgtgc
    25321 gacaccgccg ccgcatgcag cgcaacgcca tcgcccatca ccaccgtgcc ggtacgcgag
    25381 ttaaacacca cgcgggcgcg gattttctct gcctgcacct gtacatcatc cagttgcgac
    25441 ataaatgcca cccgcgcgcc ggggctggtg ggcgcacgca cgctgacgtt ggtagagctc
    25501 tgcgcagtag caatgccgcc aaacgaggcg ttaatcgccg ccgcaatatt gttggcatct
    25561 ttaaacgacg gacgtttcag gttgagggtg atagtgtcgc ccatctgaaa atcgctgggg
    25621 atctcgcgct ctaccgttgc gccgttagga attaacccgg cggttggcgt attaaccgtt
    25681 acgctcgaac cgctggcccc ggtggcagtc atcccgccca ccaccacgct gccctgcgcc
    25741 agcgcataaa cttcgccatc cgcgccgtgc aactgggtca gcaacaaagt gccgccacga
    25801 atacttttgg catcgccaat cgacgaaaca gtgacatcga tggtctgccc gcgcgagtac
    25861 atcggcggca gcgtggcgct caccgctacc gcggcgacgt ttttcacctt cggatcgatt
    25921 ttatttggca gttgcacgcc aaactggcgc agcatattgg tgatggtctg gttggtgaat
    25981 ttgacctggt ttttatcacc agtaccgtcg aggcctacca ccaggctgta gccgaccagt
    26041 tggttttcac gcacgccctg tacgttgacc aacgactcca gcgattgcgc cagcgccacg
    26101 ccgggcagcg tcagacttac agcgatcgct gcggccttta tccagttttg cattgcactc
    26161 tccggcatta aatcgggaac agaggatggt taaacagccg cgtcaaccag cctgcggcgt
    26221 tggcgtcgct taacgcgccg cgtccggcat aggagatgcg ggcatcggcg atccgctgcg
    26281 aggaaaccga gttgtcgttt tggatgtcgt cggcgcgcac caggccgctt aagcggatgt
    26341 actcatcgcc ctggttgaga gtcagccatt tttcgccgcg aatttccagg atgccgttag
    26401 gctgcaccgc atggacggaa acggtgatct ccccgcgcag gctgttctgc tgctgggaag
    26461 tggcgctgcc gtcaaagtcg cggttggcgt cgatagagcc ggaaagttta tctttggtgt
    26521 gtccaaaaat cgtgggcgcg ccgatatcga cggtgttgct tttgccgaag ttggttttcg
    26581 cctgtttgct cgactgggtg gactcttcga ggatcaccgt caggatatcg cccacgcggt
    26641 aagcccgccg gtcggcggtg agcgaccagt tgtagccggt ttcaaacacg cccccggcac
    26701 gcccgtctgc cggaggcggc gcttcggtgg ttggtggcgc aaagtatgcg tcgtcttttt
    26761 tgaccagaat ggcctgcgat tcacacccgc taaggagtgg aagcagcgcc accagccaga
    26821 gataattttt cacgttctgc ctgtttaaat acagtgagtt atgtgctctt gtaggtcgga
    26881 taagacgcgt cagcgtcgca tccgacattt cggccttaca gcgtctggct gatgtactgc
    26941 aacatatcgt ccgcagccga gaccattttg gcgttcatct cgtacgcccg ttgcaccgta
    27001 atcatcgcca ccatctcgtt gacgatatcc acgttcgatc cttccagcgc gttgtcctgc
    27061 aaggtgccaa gcccatcttc gcccggtacg ccttcggtcg gctgaccgct ggcggcggtt
    27121 tccagataga ggttgtcacc ctcagcggaa agcccggccg ggttggcgaa gttcaccagc
    27181 gtcagttgcc ctaactcagt cacgtcgctg tcgccgggta agatcgccgt caccgtgcca
    27241 tcctcaccaa aagcaacatt agtcgccccc gccggaacgt cgatttccgg ctgcaacggc
    27301 aagccctgcg agttggtcag tacgccgtca gcgttcacct gcaagttgcc gtcgcgggta
    27361 taagcgatat cgccgttggc cttttgcacc tgcaaaaacc cctgccccat aatggcgaca
    27421 ttcatcgcgt tgtcggtggt ttcgacgttg ccttcggtga aggttttctg agtgccgaca
    27481 atgcgtacgc cgctaccgaa ttgcagcccg gtcggcataa tgttgttctg atcgagcatc
    27541 gcgcccggcg cttcctgggt ctggtaaaac agatcctgaa acatcacccg gtcacgcttg
    27601 aagccggtgg tgttaacgtt ggcgatgttg ttggcaatcg cgctcatttc agcatcctgc
    27661 gccgagaggc cggttttgct gatccatagt gctgcgttca tggtggtaaa ttccgtgtta
    27721 ttttttggcc gcggttgccg gatgcggcgt gaacgcctta tccggcctac gatgtgcgat
    27781 tacgagccgc gcaataagcg gttgcccgcg tcgctgatat cttcggcggt tttcatcatc
    27841 ttgatctgcg cttcgaactg gcggttcatg gcgatggacg acatcatctc gctcaccgcc
    27901 gagacgttgc tgccctcgag aaaaccgccg ctgactttga tgttttcatc gcgctgggcc
    27961 ggaacgccgt cggcagtcac cagcatccct tcaggatttt tcgccaggtt cgcgaccggg
    28021 atatccacca gcttcaggcg gtcgatatcc atcgtcgcgg tcacgtcacc gtcatcgggc
    28081 gtcacggaga gcgtgccgtc gctgccaaac gaagcaatgg cgttgggcgg cagaatgatc
    28141 ggcccgttat ctcccagcac cagattgccg tcgatagtca gttgcccctg gtcatcctgc
    28201 tgaatgttgc cgttgcgggt atacacttcg ttgccgtttt tatcctgcac agcaatgtaa
    28261 cccgaaccct gaatcgccac gtccagtggg cgttcggttt tctccgcaac accagtgctg
    28321 tcgttcacgc cgctggcccc ttcctgcgcc atatagcgcg tgtcgaagcc gccgcctttc
    28381 accttatcgt tagtcgccat tgccatatcg gcgcgaaaac cgttggtgtt gacgtttgcc
    28441 aggttgttgg cgctgatctg ctgctcgtac agggtctgag aagccccgct cagggcggta
    28501 taaatcaagc ggtccattac attgcctgga acagcgcgtc atcgagctgc gtgctggtgg
    28561 cgataacttt ggtgttggcc tgatagttac gctgggcggt catcaggttg actaactcgc
    28621 tggtgatatc gacgttagag ctttcgagca cgcccgacga cagcgtgccg caggtgccgg
    28681 aaccgggaac gccaatcagc ggggtgccgg attcgccggt ttgtacccac gcggtgccgc
    28741 tcactgcctc caggccgttt tcattcggga acgtcgccag caccacctgc ccctgcaaca
    28801 tgcgctcgcc gttgctgtag gtagcgtaaa ctttgccatc gtcatcaacc tgcacaccgt
    28861 tctgagtggc agaggcgtag ccgtcggcgg cgttggtggt gaccgaaaat tcagagccgt
    28921 attgcgtaca ggtcgagtaa tcgacggtta aatcgatggg tgcggcggcg tcggtctgaa
    28981 actcaatggt ctgcggcgtg gttggcgagg tcagtttccc ggtgttcggg tcgaaggtta
    29041 acgtcgtcgc cggaacgccg gtctgctgct gaccgtcgaa ggtgtactgc acttcccagg
    29101 tgttgtcgct ggttttggtg aagtactggc ataccgaatg ttcgttgcct aatgagtcat
    29161 acaccgtggt ggtgtagctg tcggtgtacg agctgctgtt ggtcgcgtcg aacggtacgg
    29221 tagtgcggtc gatagccgca tcgctggcat cgaagttggc ggtaaaggtc aggctgctgc
    29281 tggcctgcgc cggaatatta ccggttttga tctggatatc ggtgactgta ccggtttgca
    29341 gggtgcccgt gtcatcgacc ggatagccct gcaaataagc gcccgaggcg ttgacgatat
    29401 agccgttttt gtcggtttca aacgatccgg cgcgggtata agaaatgttg cccgcgctgt
    29461 cgcaggtaac aaaaaagccg tcgtcgttaa tcgccagatc cagcgcgtta ccggtggata
    29521 ccagcgaacc gccgcgcgaa atactttgcg cggtgcctgc cacgctgacg cccatcgcct
    29581 gacttccggc atacatggct gaaaactggg tggtcatcga cttatagcct accgttccgg
    29641 cgttggcgat gttgttactg atcccgtcca gctgttcgtt aacggcattc agccccgttg
    29701 cagcaatttc ataactcatt gtgtcttcct gttaattcag attttggcag cgtgcccgtt
    29761 gttttgccgg atgcggcgtg aacgccttat ccggcctacg aacgggtgca aacataaaaa
    29821 tatgcgcgaa ctcgtaggcc tgataagacg cgttaagcgt cgcatcaggc atcttgcacc
    29881 gaacgccgga tgcggcgtga acgccttatc cggcctacaa aaccattcgg attcaatagt
    29941 ttcatgtagg cctgataagc gaagcgcatc aggcaatttg gcaccggaca tcacgccaca
    30001 tcactgtcgg catcgggtac gccaaactgg ctaatcatgg tgaacggcac ttcgcctaca
    30061 ccggcaacgt taagcaccgg ggagctgccg tcgagcggga tgcgcacatc ctccacttcg
    30121 ccggaaactt caatcggcac ctcttcttcc ccggtgttgg tgaccaccga gacgtcgtaa
    30181 tcgcccggcg ggataccgta atccgccgga ttaatcgaga aatccacctg acctggcccg
    30241 acggacgaac tgccttccgg gatcagcgac accgtatagt catcgcctgc ggcatcggta
    30301 aagtggagat cggcggtggt acaggcgtcg tcaagcgtgg cgcgaccatt aatggtctgg
    30361 tcgctcacct gcaaggcggt ggtctgcacc ataatgtcgt cacccaccag gttgccgagc
    30421 gccatcacct gaatgttgct catcagcacc gcgttggtgt tggcctgcac gctcatctct
    30481 tccatcatcg ccacctgcgc catcgaagaa agctggtcga tatattcggt ggcgtcagtc
    30541 gggtcggtcg ggtcctggtt ttggatctca gcgacaaaca gcgtcaggaa ggtatcgacg
    30601 ggcgagctat cgctgctact ttgcgtgtcg ctggccgcca cgttgttgct ttccgccgtg
    30661 gcggtcggct gcggtgattg cgcattcagc gccagggtat tcatcaggct tctcccagtt
    30721 tcagcaaact ttgctgcata cttttcacgt tgttgagcac atcgacattg gtttcgaaat
    30781 cgcgcgaagc cgacatcatg tccgccatct gttccaccac gttgacgtcg ggataccaga
    30841 catccccgtt agcatcggcg agcggcgaat gtggttcata gcgtttcaca gctccgccgg
    30901 tttgcagcac gtcctgcacc tgcacactgg caccgtcaat ggcatgacga tgtgtacccg
    30961 ccagcaaact gtggtgatac accgccgcaa acaccgggct gcgcgcttta tatgcctgcg
    31021 cctcgctgct ggcgggcgca ttggcattcg ccaggttact ggcaacggta ttcagacgca
    31081 gcgtttgcgc cgtcatcgcc gaaccggaaa tctgatagat atcagtaaat gacatgctta
    31141 tttcccttca atggcctctc tgatgccgct gatttgcaga ttcagaaagg tcagactgct
    31201 ttgataatcc atactatttt gtgaaaaccg cgcttgttcg ctgttcagtt ccacggtgtt
    31261 gccatcttcc gacggctgca tcggcacacg gtactggaca tccaccgccg cgttatttgc
    31321 ccgttgcatc tccctggcaa aatcaatatc tttagccttg aaatttggcg tatcgacgtt
    31381 cgccagattc gcagtcagta gctcggtcct ctcaagacgt aacttcactg cctgcggatg
    31441 tactcccaat gcctgctgaa aactgatccc catttcctgc gtcactcctg cttcaatccg
    31501 catacgctaa gcaatcgccg tgccattttt taagtgactg aagagaaagg gtttatgagt
    31561 tttcaatttt gcaggcggaa gttttatttc ctcctggcgc tggccttacc gggctacgcc
    31621 gccgtccatc ccgtgcagca cagcgcgcgg gaacaagtca acgcccaggt actgaatgcc
    31681 gccagccaaa aaattgaatc gctggcgcaa cagcggcgct ggcatgacta tcgctacacc
    31741 tttaaggtct acattccctc gcagattgcg accgccgcgc cctgtactaa aacacccggc
    31801 gttacgctga cttcgcctgg ggacatcgcc ctcaaccgga tgaacttcac cgtcagttgc
    31861 ccacaaagct ggcagatgaa cgtagcggtg cgtccggatg tactggtgcc agtagtaatg
    31921 gcgaagtcac tggttgcccg cgatacgccg ctcaccgcga atgatgtgga gttgaagcct
    31981 tacaacgtca gtgcccagcg ccgggaggtg ttgatggaac ccaatgacgc gattggtttc
    32041 agcagtaaac acgcgctgca accgggcagg ccaatcacca aagaagaact gatatctccg
    32101 gtgctggtcg agcgcgatca accggtgatg attgtttacc agagcgcggg gatcaccgcc
    32161 tcaatgcccg gcgtagcact gaaaaatggt cgcaaagggg aaacggtgaa ggtgcgtaac
    32221 gccagcagcc agcgggtcat cagcgcgatg gtggcggaaa gcggcgtggt gacaacggta
    32281 agcgcggagt aatttgcgcg ggcgattaaa gttttgccgc ctgttaaccg ctttaccgga
    32341 gtaacccacc caaaataccg agacgaggtt acgatgaaaa tcaccccgac tatgccaggc
    32401 aatcacccga cagccacaat ggcaggctcc agtcagccgc gcaaaagcac ggcgaccacc
    32461 gccacgacgc tgagcgccga tgacattacc caggcgggat tgcagtctgc ccagcaaacg
    32521 ctgaacgacc agcaagagag cgatatcgac agcgacaaag tggcacaaat gcaggcgatg
    32581 ctagcctccg gcagcctgca agtggacacc gagcagttag ccagcgacat gctgagtttt
    32641 ttccagaact aagaggccga tgaagtgagt atccggttac aacaggtgaa ggcgttgttg
    32701 cagggaattc gtgacgacga cgcactgtac gattccctgc gtgaacttct ccagcgtcag
    32761 cggatctgca tgattcgccg cgccagagag gatctgctgg cggtaaacga cgagataacg
    32821 cagcactatg agcagttgca tgcgcatagc catcagcggc atagcctgct gaaaatgctc
    32881 ggtgttagcg taaatcgtga cggtctggcg caggtttttg cctggcttcc ggcggtacaa
    32941 aaagcggcag cgcaacagtt gtggcaacgc cttgagcaaa aagcagaacg ctgcaaagcc
    33001 tataacgata aaaacggcga gctgttaatt cgccagtatg aatttattca ggctttttta
    33061 ggcagcgaag cggattttct ctatcaggga taacgacaat tccttccccc atcgcggaaa
    33121 cggattttcc gcctgatccc caatcctttg tttttgatta aaaaaattcc cggcacgctt
    33181 attgcacatc ctgcaaggta ataaagactg gaaactacct atgagtttta ttgaatgtta
    33241 cgaaccggaa tatgtgcgca attttatggc acgccacccc gattcgcctt tatacgtccg
    33301 caaggtgtgg aaaaacgaga ttcgccagag cctgatgctg acagagccag aaagctgcga
    33361 agcggtgctc aacgatgcct gcgcctttga tttacagctc acctaccgcc cggtggaaga
    33421 aaatgccgca gaactctccg cgcgcgatgc gatagtaaac caggccattt tgagcaccct
    33481 tacgctcccc gatttaaccc cggagcttta cctgtatgcc gtcggaattg tgctctcccg
    33541 cgccagtaaa atgccgggca gagatggaga tattcttgcc cgcttgacga cgctgccaca
    33601 ggcgctggcc gatcatgcac aaaaaggcac gctacaggca cagttcgccc agctcccgcc
    33661 cgtgccacag ccggcgcgcc agttgatgac gctgctcggc agttgtgcat ttgactggtc
    33721 gattttgccg gaaagcccgc gcaaaacttc cctgccgttg caaatgcctt tgctgacgct
    33781 acatgacgcc aacagcaaag ccctgttaca acagcagctt aaggtgcagt ggcaaaccac
    33841 ctggcaacag cattttgcca ccgcgccgtg gatgatgcgt aactggctga tttaccgcgt
    33901 ttaccatgag gtgattggac aggcagacgg cgcagattat tgcccgctgg tgtgcgattt
    33961 ttatcttatc cgcacgttaa tcagtttatg gacgctggat ggttctccgc tgcgacagga
    34021 ggatattttc gccctcttcg ccatgtttga acgctggcga gagagtgaaa atgccgtgct
    34081 gattcgccaa caaattcagg ctgaacgttc cgcagatccc ctgctttatg cgttttcact
    34141 gctgtcttgc taacgctttc tggccggttt tcccggcctt atcgacttcg taaactatca
    34201 tgcaaaccct catcaaaaca ctcgtcactc accgcgtaaa accgtttgtc agcgtcgtca
    34261 cgccaacctg gaaccgcggc gcattcctgc cgtacctgct ttatatgtac cgctatcagg
    34321 actacccggc agaccgccgc gaactcatca tcctcgacga ctccccgcaa agccatcaac
    34381 acatcatcga ccgcctgacc aatggcacgc cggaatcctt caacatccgc tacatccatc
    34441 acccggaaaa actgccgctg ggcaaaaaac gcaatatgct caacgaactg gcgcgcggcg
    34501 aatacatcct ctgcatggac gatgacgact actactcgcc agataaaatc tcttactcca
    34561 tcgccatgat gcaaaaacac cgggcgctca tctccggttc cgaccagatc cccatctggt
    34621 acagccacat caaccgcatt ttcaaaagcc gcagctttgg cccacataac atcctcaatg
    34681 gcaccttttg ctatcaccgt aactacctga aaaagcaccg ttacgacgac gattgtaatt
    34741 taggcgagga gaaaagtttc accgacaatt tcagcgttaa cccgctgcaa ctgcccggcg
    34801 agcgcacgat tctgtgtatc tcgcacagcc acaacacctt tgataaagac tttatcctcg
    34861 gggccagcac gcctgtaaac gcggtgctta cggatatcgt cagcgatccg ctgctacgca
    34921 acgcttatct gggtctgcat aacgccactc attatcaggc gatccaccat caggcgatcg
    34981 atcaggtcgt attgatcaat ctggataaac gcccggatcg cttacagcag atccgtgaag
    35041 aactgacatt gctgcatatt ccgcctgaga agatcacccg ccttgccgcc agtgaaaatg
    35101 aaaacggtca acggggccgc cagcaatcac acttgcaggc gctgcgtctg gcgcaacagc
    35161 acggctggca aaactacctg ttgctggaag atgacgcggt tattctcaaa caggagaaac
    35221 atattcaggt gctgaacgcg ctgctggcga gtctggcgaa aattccctgg caggtgatga
    35281 tcctcggcgg cgaaatttcg cagggaacga tgctaaaaag tttgcctggg ctggtgcacg
    35341 cgcgggactg ccgcaaagtg tgcgcttatc tggtgaacag ccgctactac ccgcagctgg
    35401 cgcagcagat gaacaacgat gagcattcgc tggaagacgg ctggcagccg ctgctgcaca
    35461 ccgacaaatg gctggcctgt tacccgagcc tttgttatca gcgacagggc tttagtgata
    35521 tcgagaaaaa agcgactgat aacattaacc actacttcaa taagttaccc gtagccacca
    35581 agccatctac attacctatc gccgatacca tcggcttttt tatggaaacc tcattccact
    35641 acgcgctcta ccgcccaatc atcaccgccc tgcaggcgca ggggcagagc tgtacgctgg
    35701 tgatcaatga ccgcgtattt aaaccatttc tggatgaaat gctggcgacg ctaaaaagca
    35761 tcgacgatcc gcagctcaaa ggaatgcgct tatctgaaat gcaggcacac ggtcagcgag
    35821 tgaaatgcct ggtcagcccc tatcacacgc ccgcgctcaa tggcctttca accctgaaca
    35881 ttcgcgccat gtacggctta gccaaagaaa cgtggaacca cgccgactgg aaccgttttt
    35941 atcagcgcat tttgtgttac agccactaca gccagcgggc gctggcacat ttcggcagcg
    36001 caaaagtggt cggcaatccg cgcttcgacg cctggcataa cggcacgttt gaccgcgctc
    36061 tgccggaaaa tatccaaccc gattcccata aacctacggt gctctacgcc cccacgtttg
    36121 gcgcattaag ctccctgccc cactgggcag aaaaattggg ccgcttaagc ggtgatgtga
    36181 acctgatgtg caaactgcac cacggcactt gctcgcgccc ggaagaagct gcatcgctgg
    36241 cactggcgcg caggcattta aaacagcgca ccgactctgc ccgccacacg ctggcgctac
    36301 tggcaaaggc cgattacgtg ctgaccgata acagcggctt tattttcgat gctattcacg
    36361 ttgataagcg ggtgatcctg ctcgactttc cgggcatgac aacgttgctc gacggtgaga
    36421 aaagttattc cacgcccgaa agtgccgacc agcaaattcg cgaaatattg ccggtggctc
    36481 atgatgttgc ggaattacgt tatctgctat cagaggcgtt tgactggtgt gcggtacagg
    36541 ggcaactggc ggaaattcgt catcactatt gcgatgcgtt tatggatggc aaggcgggag
    36601 aacgggcggc gatggtgatt atggaggcgc tggggtaaac cgcgcaagtg cctgatgcgc
    36661 ttcgcttatc aggcctacgc gcccctgcaa tatagtgaat ttgcgatgat ttgtcggccg
    36721 gaaaaggcgt ttacgccgca tccggcacga acagtgcact tatctaccct tcgatttcaa
    36781 atacatcgct ttatggctgg ttaatcggta aaagttgcac attttgatag cgagaagagc
    36841 tgtctttatg aattccgaaa agcgcccttc taccctctat aaccattctt ttttacctgt
    36901 attggccgca acatacctca cccacgaagt ggttgtagca attcgtgggc taaaggtccg
    36961 cattatatga aacgggaggg cggtagcatt tctgatatta catgcctcaa ttttgctgaa
    37021 ctattggcta tcctggacct caaaaatctg gtcataagat agatttttta agggaatacg
    37081 gagtaaaggt gtggaaaact aattaatagg tttgtcttaa tgcttcagaa gattggatac
    37141 atggaagaag ataatattat agagaaaaaa ccatggcgtg ggatcccacc tgaatacacg
    37201 gatagatgga gggaaattta taacaacgct gctcccgaag aagggatgca tttatcagat
    37261 gcttgtccag tatgtggtga tacggaatta tatcgctatt attctctgtt aaaaacgcaa
    37321 ccacgcttat taggagggat tatgtataaa ggacctggaa gttattggga atggtgtggg
    37381 tcttgtcgtt cctatgaaca tatgtcaggc tatgtaccaa tatcgtgggt tgtggagtta
    37441 tcaggaatag atcattcaaa attaacggcc attccagata tgattgatat tgcaagacat
    37501 aaaaatcatt acttctctta aatattatct aattagtttt cccggacagg gcaagttggc
    37561 gaaaatagtg aggagcatca cgttttccat cctgactgac acgcctactg gtgattaaaa
    37621 tcaaacctat ggaatgacga aaaagatgga aaggaaaagc atatcaagta atcagtcagg
    37681 aagatattca caacaatcac ctgaatcagt agatcgaatg aaggatcact tgtcacataa
    37741 tataattgtc gatatagatc ttaattcacc aggagttgtt actaaactcg atttattatt
    37801 aagaaaaaat aaaatatcaa aaacatgtct ctgcgggatg tttccagtat ctgccc
//
LOCUS       DAJOTB010000028        37834 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJOTB010000028 DAJOTB010000000
VERSION     DAJOTB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.028"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            91..1485
                     /gene="galP"
                     /locus_tag="OHN28_001465"
     CDS             91..1485
                     /gene="galP"
                     /locus_tag="OHN28_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCQ2296883.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1562..2059
                     /locus_tag="OHN28_001466"
     CDS             1562..2059
                     /locus_tag="OHN28_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCQ2296884.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2154..2861
                     /gene="endA"
                     /locus_tag="OHN28_001467"
     CDS             2154..2861
                     /gene="endA"
                     /locus_tag="OHN28_001467"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCQ2296885.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2941..3672
                     /gene="rsmE"
                     /locus_tag="OHN28_001468"
     CDS             2941..3672
                     /gene="rsmE"
                     /locus_tag="OHN28_001468"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCQ2296886.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQVLQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPVMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3685..4635
                     /gene="gshB"
                     /locus_tag="OHN28_001469"
     CDS             3685..4635
                     /gene="gshB"
                     /locus_tag="OHN28_001469"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCQ2296887.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4744..5307
                     /locus_tag="OHN28_001470"
     CDS             4744..5307
                     /locus_tag="OHN28_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCQ2296888.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5307..5723
                     /gene="ruvX"
                     /locus_tag="OHN28_001471"
     CDS             5307..5723
                     /gene="ruvX"
                     /locus_tag="OHN28_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCQ2296889.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5897..6877)
                     /locus_tag="OHN28_001472"
     CDS             complement(5897..6877)
                     /locus_tag="OHN28_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCQ2296890.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            6895..7599
                     /gene="yggS"
                     /locus_tag="OHN28_001473"
     CDS             6895..7599
                     /gene="yggS"
                     /locus_tag="OHN28_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCQ2296891.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKICYFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7617..8183
                     /gene="yggT"
                     /locus_tag="OHN28_001474"
     CDS             7617..8183
                     /gene="yggT"
                     /locus_tag="OHN28_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCQ2296892.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8180..8470
                     /locus_tag="OHN28_001475"
     CDS             8180..8470
                     /locus_tag="OHN28_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCQ2296893.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8478..9071
                     /locus_tag="OHN28_001476"
     CDS             8478..9071
                     /locus_tag="OHN28_001476"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCQ2296894.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9064..10200
                     /gene="hemW"
                     /locus_tag="OHN28_001477"
     CDS             9064..10200
                     /gene="hemW"
                     /locus_tag="OHN28_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCQ2296895.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLNNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            10515..11501
                     /locus_tag="OHN28_001478"
     CDS             10515..11501
                     /locus_tag="OHN28_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCQ2296896.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKNQTHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQIIETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRKAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            11546..12049
                     /locus_tag="OHN28_001479"
     CDS             11546..12049
                     /locus_tag="OHN28_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2296897.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYILNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINFLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            12049..13350
                     /locus_tag="OHN28_001480"
     CDS             12049..13350
                     /locus_tag="OHN28_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCQ2296898.1"
                     /translation="MDSYIALTLFGCFFVLVFLGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMVVCAIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13406..14413)
                     /locus_tag="OHN28_001481"
     CDS             complement(13406..14413)
                     /locus_tag="OHN28_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCQ2296899.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADVYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14530..15576)
                     /gene="ansB"
                     /locus_tag="OHN28_001482"
     CDS             complement(14530..15576)
                     /gene="ansB"
                     /locus_tag="OHN28_001482"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCQ2296900.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(15752..16471)
                     /locus_tag="OHN28_001483"
     CDS             complement(15752..16471)
                     /locus_tag="OHN28_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCQ2296901.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPHDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(16655..16981)
                     /locus_tag="OHN28_001484"
     CDS             complement(16655..16981)
                     /locus_tag="OHN28_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCQ2296902.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAVVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            complement(16981..17700)
                     /gene="trmB"
                     /locus_tag="OHN28_001485"
     CDS             complement(16981..17700)
                     /gene="trmB"
                     /locus_tag="OHN28_001485"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCQ2296903.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            17861..18913
                     /gene="mutY"
                     /locus_tag="OHN28_001486"
     CDS             17861..18913
                     /gene="mutY"
                     /locus_tag="OHN28_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCQ2296904.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLASAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQISADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            18941..19216
                     /locus_tag="OHN28_001487"
     CDS             18941..19216
                     /locus_tag="OHN28_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCQ2296905.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            19281..20360
                     /gene="mltC"
                     /locus_tag="OHN28_001488"
     CDS             19281..20360
                     /gene="mltC"
                     /locus_tag="OHN28_001488"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCQ2296906.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            20562..21818
                     /gene="nupG"
                     /locus_tag="OHN28_001489"
     CDS             20562..21818
                     /gene="nupG"
                     /locus_tag="OHN28_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCQ2296907.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21867..24002)
                     /gene="speC"
                     /locus_tag="OHN28_001490"
     CDS             complement(21867..24002)
                     /gene="speC"
                     /locus_tag="OHN28_001490"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCQ2296908.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKSTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLMQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVVPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            24401..25108
                     /locus_tag="OHN28_001491"
     CDS             24401..25108
                     /locus_tag="OHN28_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCQ2296909.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            25214..25289
                     /locus_tag="OHN28_001492"
     tRNA            25214..25289
                     /locus_tag="OHN28_001492"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25247..25249,aa:Phe,seq:gaa)
     gene            25466..26650
                     /locus_tag="OHN28_001493"
     CDS             25466..26650
                     /locus_tag="OHN28_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2296910.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSIQ
                     ES"
     gene            26800..27810
                     /locus_tag="OHN28_001494"
     CDS             26800..27810
                     /locus_tag="OHN28_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2296911.1"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGY
                     RPNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDE
                     HADCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDF
                     DNHAGTGQATQKLIALGHKRIALLSEDTSHSYVVARRQGWLDALNEHELEDTWLRLVS
                     PTRRAGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKDAVSLVVYDGL
                     PQDSIIELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHPP
                     CS"
     gene            27906..30032
                     /locus_tag="OHN28_001495"
     CDS             27906..30032
                     /locus_tag="OHN28_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2296912.1"
                     /translation="MVSKYCRLSSPRSDLIIKTRPHAEIIWWGSALKHFSPDDCASLE
                     RPVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDASPVFYTVDVEHTENTLRLT
                     SEDSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFH
                     GRWTREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWS
                     GNHRMRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFK
                     GRNDDRAALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPD
                     WVLSMPGYSQPTGRYQYVLNLNIPEAFAYIYERFLWLLGEHPVDYVKWDMNRELVQAG
                     HEGRAAADAQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNND
                     ALERCTIQRGMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVA
                     ADAKESDGYRRYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQL
                     AMPDYTLPGILRFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGE
                     WLAQGGIQLPVLDPESAILIALERAV"
     gene            30095..31372
                     /locus_tag="OHN28_001496"
     CDS             30095..31372
                     /locus_tag="OHN28_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296913.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLL
                     RFNIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCAAM
                     AGMLFNVDPSLVFWMGSGSALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFS
                     LFRMRQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNA
                     IIMFCTPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIA
                     LTVTVISVFTLSSSRGSVHPSVEKAPAAHSEIN"
     gene            31369..32802
                     /locus_tag="OHN28_001497"
     CDS             31369..32802
                     /locus_tag="OHN28_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCQ2296914.1"
                     /translation="MMKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLI
                     YFNGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPIALAPGDKYDRDGCFSGSA
                     VDDDGVLSLIYTGHICLDDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFR
                     DPKVWHEDGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGKSYMWECPD
                     FFRCGNFHWLMFSPQGMPPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDF
                     YAPQSMLAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREV
                     ESLRKKCQPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDN
                     QAGRLVLWRYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYP
                     QADSRQLSLYAAHGDAILTDGTLWMLT"
     gene            32997..34391
                     /locus_tag="OHN28_001498"
     CDS             32997..34391
                     /locus_tag="OHN28_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2296915.1"
                     /translation="MNKLLLGLSLFLLLEYNACAAKTGGLTLEQRMALLEERLEAAEK
                     RAEKAERMLKSFDIEQHSEIRQISSEQNKKDANSYAVVESTKEKTSSPGFLLSGYNDL
                     KFYGDVEFNIDAASKPGQLVMISSGANSESVNERWDLNGRILLGFDGTRKLDNGYFAG
                     FSAQPLADMHGSVNIDDALFFFGKDDEWKVKVGRFEAYDMFPLNQDTFIEYSGNTAND
                     IYADGRGYIYMMKEGRGRSDAGGNFLISKQLDNWYFELNTLLEDGTSLYNNGNYHGRY
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVKNAYGYTDNKGNFVDQSNRSGYGIS
                     MTWNGLKTDPENGIVVTLNTAYLDASNEKDFTASINALWKRFELGYIYAHNKIDEFSG
                     VVCNNGCWIDGEGIYNIHTIHASYQFANVMDMENFNIYLGAYYSILDSNCRYSNCDIT
                     DDRYGARLRFKYFF"
     gene            complement(34969..35256)
                     /locus_tag="OHN28_001499"
                     /pseudo
     CDS             complement(34969..35256)
                     /locus_tag="OHN28_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(35245..35606)
                     /locus_tag="OHN28_001500"
                     /pseudo
     CDS             complement(35245..35606)
                     /locus_tag="OHN28_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            35656..36690
                     /locus_tag="OHN28_001501"
     CDS             35656..36690
                     /locus_tag="OHN28_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2296916.1"
                     /translation="MTRFEVNNNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISETGY
                     INKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSGEII
                     WMHPENRPYLRALYATILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSELLRT
                     GDHERAFSVLKKHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEMLCGN
                     LHPFPLAVRHNFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAEIIK
                     CAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINDEYVWPWILPFSAA
                     GMKHSSIQHQ"
     gene            complement(36834..>37834)
                     /locus_tag="OHN28_001502"
     CDS             complement(36834..>37834)
                     /locus_tag="OHN28_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2296917.1"
                     /translation="QRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYS
                     RPTTPELQKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQ
                     AGFDTWWLSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKL
                     IVLHLTGSHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASV
                     MYFSDHGLEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPTLGEHVDRQTVNSVFSTA
                     YNDYLINAWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELQE"
BASE COUNT         9198 a         9296 c         9397 g         9943 t
ORIGIN      
        1 gattacactg atgtgatttg cttcacatct ttttacgtcg tactcaccta tcttaattca
       61 caataaaaaa taaccatatt ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca
      121 aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc tggcgggatt actctttggc
      181 ctggatatcg gtgtaattgc tggcgcactg ccgtttattg cagatgaatt ccagattact
      241 tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg
      301 ggcagcggct ggctctcctt taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc
      361 ctgtttgttg ccggttcact gttctctgcg gctgcgccaa acgttgaagt actgattctt
      421 tcccgcgttc tgctggggct ggcggtgggt gtggcctctt ataccgcacc gctgtacctc
      481 tctgaaattg cgccggaaaa aattcgcggc agtatgatct cgatgtatca gttgatgatc
      541 actatcggga tcctcggcgc ttatctttct gataccgcct tcagctacac cggtgcatgg
      601 cgctggatgc tgggtgtgat tatcatcccg gcaattttgc tgctgattgg tgtcttcttc
      661 ctgccagaca gcccacgttg gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg
      721 ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa
      781 agtttgcagg ttaaacagag tggctgggcg ctatttaaag agaacagcaa cttccgccgc
      841 gcggtgttcc ttggcgtact attgcaggta atgcagcaat tcaccgggat gaacgtcatc
      901 atgtattacg cgccgaaaat cttcgaactg gcgggttata ccaacaccac cgagcaaatg
      961 tgggggacag tgattgtcgg cctgaccaac gtacttgcca cctttatcgc aatcggcctt
     1021 gttgaccgct ggggacgtaa accaacgcta acgctgggct tcctggtgat ggctgctggt
     1081 atgggcgtac tcggtacaat gatgcatatc ggtatccact ctccgtcggc gcagtatttc
     1141 gccatcgcca tgctgctgat gtttattgtc ggttttgcca tgagtgctgg tccgctgatt
     1201 tgggtactgt gctccgaaat tcagccgctg aaaggccgcg attttggcat cacctgctcc
     1261 actgccacca actggattgc caacatgatc gttggcgcaa cgttcctgac catgctcaac
     1321 acgctgggta acgccaacac cttctgggtg tatgctgctc tgaacgtact gtttatcctg
     1381 ctgacattgt ggctggtacc ggaaaccaaa catgtttcgc tggaacatat tgaacgtaat
     1441 ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc
     1501 tcccgtcgtg gaggaagcca cctcttgcag tcatcttttc ttcgctctat cctctgccgc
     1561 tatgaaaaca tcccgtctcc ctatcgccat ccaacaggcc gttatgcgtc gcctgcggga
     1621 aaaactcgcc caggccaacc tgaagctagg gcgtaactac ccggagccaa aactctctta
     1681 cacccagcgc ggaacctctg ccggaacagc ctggctggaa agctatgaaa ttcgcctcaa
     1741 tcccgttttg ctgttggaaa acagtgaagc ctttattgaa gaagtggtgc cgcacgaact
     1801 ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg ccacatggca aagagtggaa
     1861 gtggatgatg gaaagcgtgc tgggcgttcc cgcccgccgt acgcatcagt tcgaactgca
     1921 atccgtgcgc cgcaacacct tcccctaccg ctgcaagtgc caggagcatc agcttaccgt
     1981 acgccgtcat aatcgcgtag ttcgtggcga agccgtctat cgctgtgttc actgcggcga
     2041 acagctggtt gcgaaataac cacctgaact atcaggaact ttcctgatct ggctgattgc
     2101 ataccaaaac aactttcgct acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc
     2161 gttatttgtc tattgctgcg gtggtactga gcgcagcatt ttccggcccg gcattggccg
     2221 aaggtatcaa tagtttttct caagcgaaag ccgcggcggt aaaagtccac gctgacgcgc
     2281 ccggtacgtt ttattgcgga tgtaaaatta actggcaggg caaaaaaggc gttgttgatc
     2341 tgcaatcgtg cggctatcag gtgcgaaaaa atgaaaaccg tgccagccgc gtagagtggg
     2401 aacacgtcgt tcctgcctgg caattcggtc accagcgcca gtgctggcag gacggtggac
     2461 gtaaaaattg cgccaaagat ccggtctatc gcaagatgga aagcgatatg cataacctgc
     2521 agccgtcagt cggtgaggtg aatggcgatc gcggcaactt tatgtacagc cagtggaatg
     2581 gcggtgaagg ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa aaagttgccg
     2641 aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta cttctatatg cgtgaccaat
     2701 acaacctgac actctctcgt cagcaaacgc agctgttcaa cgcatgggac aagatgtatc
     2761 cagttaccga ctgggagtgc gagcgtgatg aacgcatcgc gaaggtgcag ggcaatcata
     2821 acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta acctacacta gcgggattct
     2881 ttttgttaac ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg atttttaact
     2941 atgcgtatcc cccgcattta tcatcctgaa ccactgacca gccattctca catcgcgctt
     3001 tgcgaagatg ccgccaacca tatcgggcgc gtactgcgta tggggccggg acaggtgctg
     3061 caattgtttg acggcagcaa ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa
     3121 agcgtggaag tgaaggtgct ggaaggccag atcgacgatc gcgaatctcc gctgcatatt
     3181 cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc
     3241 gaactcggtg taagcctcat tacgccactt ttttctgagc gctgcggcgt taaactggat
     3301 agtgaacgtc tgaacaagaa gctccagcag tggcagaaga ttgcaattgc tgcctgtgag
     3361 cagtgtggcc gtaaccgggt gccggaaatc cgtccagtga tggatctgga agcctggtgt
     3421 gcagagcagg atgaaggact gaaactgaat cttcacccgc gcgccagtaa cagcatcaat
     3481 acgttgccgt taccggtcga acgcgtccgc ctgctgattg gcccggaagg cggtttatcg
     3541 gcagatgaaa ttgccatgac tgcccgctat caatttactg atatcctgtt gggacctcgc
     3601 gttttgcgta ctgagacaac tgcgctcacc gccattaccg cgctacaagt gcgttttggc
     3661 gatttgggct aacggagaag aataatgatc aagctcggca tcgtgatgga ccccatcgca
     3721 aacatcaaca tcaagaaaga ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt
     3781 tacgaacttc actatatgga gatggccgat ctctatttga tcaatggtga ggctcgcgct
     3841 cgtacccgta ctttgagcgt cgaacagaac tacgataaat ggtacgagtt cacgggtgag
     3901 caggatctgc cgctggccga tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat
     3961 accgagttta tctacgcgac ctatattctg gaacgtgccg aagagaaagg gacgctgatc
     4021 gttaacaagc cgcagagcct gcgcgactgt aacgaaaaac tgttcactgc ctggttctct
     4081 gacttaacgc cagaaacact ggtcacgcgc aataaagcgc agttgaaagc gttctgggaa
     4141 aaacacagcg acatcattct taagccgctg gacggtatgg gcggcgcgtc gattttccgc
     4201 gtgaaagaag gtgatccaaa cctcggcgtg attgccgaaa ccctgactga gcatggcact
     4261 cgctactgca tggcgcaaaa ttacctgcca gccattaaag atggcgacaa acgcgtgctg
     4321 gtagtggatg gcgagccggt gccgtattgc ctggcgcgta ttccgcaggg gggcgaaacc
     4381 cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg
     4441 aaaatcgccc gtcagattgg gccgacgctg aaagaaaaag ggctgatttt tgttggtctg
     4501 gatatcatcg gcgaccgtct gactgaaatt aacgtcacca gcccaacctg tattcgtgag
     4561 attgaagcag agtttccggt gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt
     4621 ttacagcagc agtaacccac cttagcgaga aggatctcgt tgagactctg agtgacagcg
     4681 cccttctttc cacgcatact gggcgctgtt gcttttttga accaggaaac agaacctctg
     4741 acaatgaatt tacagcatca ctttcttatt gccatgcctg ctctccagga tccgattttc
     4801 cgtcgttccg tggtctacat ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc
     4861 aacaagccgc tggaaaatct caaaattgaa gggattctgg aaaagctgaa gatcacgccg
     4921 gagccgcgtg atgaatcaat ccgtctggat aaaccggtta tgctcggcgg tccgctggct
     4981 gaagatcgcg ggtttatttt gcatactccg ccctccaatt ttgcttccag cattcggatt
     5041 tcagacaaca cagtgatgac cacttcccgc gatgtgctgg aaacgctcgg caccgataaa
     5101 caaccgtctg acgtattggt ggctctgggt tatgcctcct gggagaaagg tcaactggaa
     5161 caagaaattc tcgataacgc gtggctaacg gccccggcag atctgaatat tctgttcaaa
     5221 acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc
     5281 atgcctggtg tggcaggaca cgcctgatga gtggaacctt actcgccttc gacttcggca
     5341 ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg caccgctcgc cctttgcctg
     5401 caattaaagc gcaggacggt acgccggact ggaacattat cgagcgttta ctgaaagagt
     5461 ggcagccgga cgaaattatc gtcggtttgc cgctgaatat ggacggtacc gaacagccgt
     5521 taaccgccag agcgcgtaaa tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa
     5581 agctccatga cgagcgtctt agcaccgtgg aggcacgttc cggtctgttt gaacagggcg
     5641 gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc tgcggttatt attctcgaaa
     5701 gctatttcga gcagggatat taaggcgatt tactagcgca cagatttctg acaatgtgct
     5761 cgttgtttca tgttggatgc ggcgtaaacg ccttatccaa cctacagtga gtgcgaattc
     5821 aaagttacgc ttcccttgta ggcccgataa gcttgcgcat cgggcatatt tacaattcgt
     5881 atcagacaca gcaacgtcac aaacgccctt cccccacccg ctgctgataa ctctgctgaa
     5941 acgttatcat ccccacctgc tgcccggttt gaataacatg cggcaactgg tgggttttcc
     6001 cttctcgaat caaattcccc accgcgggtg tgttaatcag taattcaaac agcgccacgc
     6061 gtccttcctg tttatccact tccagttttt gtgacaacac tgcccgtaaa ctacctgcca
     6121 gttgattacg cacgggatct ttttcctgcg caggaaatga atccaccagt cgctcaactg
     6181 cctgcgccgc accacgcgta tgtaatgttg ccagcaccag atgcccggtt tctgccgccg
     6241 tcagcgccag acggattgtc tcgctatcac gcagctctcc gagcaaaatc acatcaggat
     6301 cttcccgcaa tgcagcccgc aatcccgatg cgaaagtcat acagtgcaaa ccaatctccc
     6361 gctgttggat caaacatcgc tgactggtat agagatattc cacaggatct tccagcgtca
     6421 gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc gtggtagatt
     6481 tgccactccc cgtcgccccc gtcaccagaa tcaggccatt ctcgctcttg agtaattccg
     6541 gcaataccgg tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt aacagccgta
     6601 acgccagaga aataccttgc cgttgtgcga acgcactgcc gcgcaatcgc tggttttccg
     6661 ccagcgacac agcaaaatcc agctgaccat tctccagcaa tattgcccgc tgatcgtcat
     6721 ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca gcttccattc
     6781 tcccgcgaat acgccatcgt gcgggccagg cgctgcacag gtgtagatcc gagacgttat
     6841 gctttacact aagggccaca atttcttcca tattcatact aagatcctcg gaaaatgaac
     6901 gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc aacgcgttgc
     6961 ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc
     7021 atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt tcaggaaggg
     7081 gtagataaaa tttgctactt tcaggaactg ggcgtaacag gattagaatg gcattttatt
     7141 ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg gtgtcatacc
     7201 atcgaccgtt tgcgcatcgc tacccgtctg aacgaccagc gcccggcaga acttccccct
     7261 cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg gattcaactg
     7321 gctgaactgg acgagctggc agctgcggtc gctgaactac cgcgtttacg tctgcgcggg
     7381 ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa
     7441 atggctgtag catttgccgg gctgaaaacg cgctacccgc atatcgacac gctctctctg
     7501 ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt tcgtatcggc
     7561 actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg aacgccatga
     7621 atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg ctgttattac
     7681 gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag tttgtagtga
     7741 aggtaacgca gccgattatc gggccactgc gccgcgttat tccggcaatg ggacccattg
     7801 atagcgcctc gctgctggtt gcctatattc tcagtttcat taaagccatc gtgctgttta
     7861 aagtggtgac cttcctgcca atcatttgga ttgctggttt attgattctg ctgaaaacca
     7921 tcggcctgct gatattctgg gtcctgctgg tgatggcgat tatgagctgg gtaagccagg
     7981 gtcgtagccc gattgaatac gtgctgattc agctggccga tccgctgctg cgcccgattc
     8041 gccgcttgct accggcaatg ggtgggattg atttctcgcc gatgatcctc gttctgctgc
     8101 tgtatgtcat caatatgggt gtcgcagaag tcttgcaggc gaccggaaat atgctgctgc
     8161 cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc tggttttacg
     8221 gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg gcgacgaggt
     8281 aaaagtcgcc attaccgcgc cgccggttga cggccaggcc aacagccatc tggtgaagtt
     8341 tctcggtaag caattccggg ttgccaaaag ccaggtggta attgaaaaag gcgaacttgg
     8401 ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt
     8461 aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc
     8521 gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa acagacctcg
     8581 gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt ctgaaagcgc
     8641 gccatgcggc aaaagtgacc ggtttaccgg caattgccga cgactccggt ctggcggtag
     8701 atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg
     8761 atcaaaagaa tctgcaaaaa ctgctggaaa caatgaaaga cgtaccggac gaccaacgtc
     8821 aggcgcgttt ccactgcgta ctggtatatc tgcgtcacgc ggaagatccc actccgctgg
     8881 tgtgccacgg cagctggccg ggtgtgatta ctcgagaacc agcgggtact ggtggctttg
     8941 gttatgatcc aattttcttc gttccttccg aagggaaaac cgctgccgaa ctgacccgcg
     9001 aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg gacgctttac
     9061 gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa
     9121 tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca cgacgattat
     9181 gttcagcatc tgcttaacga tctgaacaac gatgtggctt acgctcaggg ccgtgaagta
     9241 aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc gatgcaaacg
     9301 ctgctggacg gcgtacgtgc gcgtttgccg ctggcagcgg atgcagaaat tacgatggaa
     9361 gcgaatcctg gtacggttga agccgatcgc tttgttgatt atcagcgtgc tggtgtgaac
     9421 cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact tgggcgcatt
     9481 catggcccgc aagaagcgaa acgagctgcg aagctggcga gcggtttagg gttacgtagc
     9541 tttaaccttg atttgatgca tgggctgccg gatcaatcac tggaagaggc gctaggcgat
     9601 ctgcgccagg ctattgaact gaatccgccg catctttcct ggtatcaact gaccatcgaa
     9661 cctaatacgc tgtttggctc tcgccctcct gtactgccgg acgatgacgc gctgtgggac
     9721 attttcgaac aggggcacca gttattaacc gcagcgggtt atcagcaata tgaaacttcc
     9781 gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg ctttggtgac
     9841 tacatcggta ttggctgcgg cgcgcatggc aaagtgacct tcccggatgg gcgcattctg
     9901 cgtaccacca aaacgcgtca tccacgtggt tttatgcagg gaagatatct ggaaagccag
     9961 cgtgatgtcg aagccgcaga taaaccattt gagttcttta tgaatcgctt ccgtttgctg
    10021 gaacctgcgc cgcgcgtgga gtttagccag tatactggcc tttcagaaga ggttattcgc
    10081 cctcagttag acgaggctat tgctcagggt tatctcacag aatgtgcgga ttactggcag
    10141 ataacggaac atggtaaatt attcttaaac tcacttcttg aattattcct cgctgagtaa
    10201 tcattaatgc gcctgcgggc gcattaatca cgtcttttat aaatgcccgc taattaactt
    10261 cggcaaagat atctctctta ctgaataaat atcaactgta attattatat ttaatgtgtg
    10321 aattaattct cattatttat tgattataaa aatgtacaac atagcgcaca aaattaatac
    10381 ttaatttctg actttatttc aaattgtgat aaacatagaa aagttgtcag acaggttaac
    10441 aaatttgtcg tacaagtcgc taataagcac aattagatat caaccaattt ccatccttca
    10501 acagggagtt ttttatgaag atgaggtcat ttacccgctc attagtctgc gcatccttgc
    10561 tggctctggt tagcactggc gtaaacgccg cagaaaaagt gacgttaaag ttagcacaca
    10621 acctggaaag aagtcatgtt gtgcatcaga gttttgaaga actggcgaaa gaggtaaaac
    10681 aactgtcaaa aggtaatatg gtgatccgca tttatcccag cagtcagatg ggtaatgcgc
    10741 gagaaaccat ggagttgttg caaaatggcg cactggatat gaccaaaggt tccgccagcg
    10801 atctggagtc ttttgataat atttatgcca tctataattt gccgttctta tttaaaaatc
    10861 agactcactt caacaaagtt gtttttggtg aagttggtaa agaaattatg gattcaacaa
    10921 aagataaagg cttttttgct ttatcggctt atgtcgctgg tacacgcagt ttctatgcga
    10981 aaaaaccgat taccaaacca gaagacctta aagggctgaa aattcgtgtt caaccaagtc
    11041 cgaccaccat caaaatgatt gaactaatgg gtgggtcacc aaccccgatt tcatttggcg
    11101 aagtttatac tgcgatgcaa caaggcgtag tggatggagc agaaaataat gtaccttcct
    11161 gggtgcagac tcgacatatc gaaattgcca aagtcttttc tgaagatgaa cacgcctcaa
    11221 ttcctgactt tttggttatc tccaccaaaa catggaataa attaaccccg gaacagcaac
    11281 aaattatcga aacagcggcg aagaaatccg aagcttatca acaaaaattg tgggaaaaaa
    11341 ttgacgctga cactcgtgct caggcgaaag ctatgggggg tgaaattgta aaagtggata
    11401 aagcaccatt ccgtaaagcc gtgcaacccc tttttgacga ctttaaaaaa gatccaaaac
    11461 aagctgcatt gttagaaaaa ttcgacaacg ctgctcaata atattccggg ccagtctcag
    11521 gccctgattt tccaggatgc ttacaatgat atttaaccgc ctgaaacttg cggtagaccg
    11581 cgtcattgcc gcgttttctg tcgccgtaat gcttgctctg gttgtatgcg tagtctggca
    11641 agtatttagc cgctacatac ttaaccagcc aagtacatta accgatgagc tggcacgctt
    11701 tttgatgatt tgggtaggat tattaggtgc tgcatatacc gtcggcgcgc agcgacatct
    11761 ctctattgat ttatttgctc tcgccctgaa taagcgcaaa caattacttc ttagtatagt
    11821 cattaacttt ctcattttag gctttgccgg ttcggtcatt gtgacaggtg gcctgaaatt
    11881 aattgataaa acgctggcaa catcacaagt atctgccgcg atgcaaatac cgatgggata
    11941 cgtatatatc attctgccat taagtggagt ggtaatgatg ttttatgctt tgtgttttat
    12001 taatcaaagc attcaacaac tgaaacaacc tgcgcaggag gccagttaat ggatagttac
    12061 atcgcgctta cactgtttgg ctgttttttt gtgctggtct ttcttggtgt gcccatttcg
    12121 ttctcaatcg gtattgcgac tgtagcttca atgcttttga tgtttccctg ggatatcgcg
    12181 gcaatcactg tttcgcaacg actggcgaat ggactggata actttgccct gttggcaatc
    12241 ccgtttttca tctttgccgg aacgctgatg aacagcggcg gtattgcgat acgtttgatt
    12301 aacctcgccc aggtaatggt tggccgcgtt cctggttcgc ttggtcatgt gaacgtcctg
    12361 gcgaatatga tgtttggttc tatttcaggt tcagccgttg cggcagctgc tgccgttggt
    12421 ggcacactga atcccattca gaccaaagaa ggttatgatc cggcattttc taccgctgta
    12481 aacgtctcat cctgtattac tggattactg ataccgccct caaacgtgtt aatcgtgttt
    12541 tccctgacgg ctggtggtgt ttctgtagca tcactattta tggcgggata tttgccaggg
    12601 atcttaatgg gtctcgcggt aatggttgtc tgcgccatta ttgctaaacg ccgtggttac
    12661 ccactttcag aaagagcaac ctttgctcag gcatgtaaag cgttcctgga tgccttacca
    12721 agtttgcttc tggtgttcat tgtaatgggc ggcattctgg gcggcatttt taccgcaact
    12781 gaagcgtcag caattgccgt tgtctacaca tttattctct cggtgctgat ttaccgggaa
    12841 gtaaaatggc gcgatttacc caaactgatt ctggaatcgg ttgttaccac ttcgattgta
    12901 ttactgttga tcggtttttc cgtgggcatg tcctgggcaa tgaccaatgc tgatattcct
    12961 tatatgatca gcgacgcatt gatggggatt tctgataacc cactgatcat attgctgctg
    13021 attaatatcg tcttattaat tgtcggcatc tttatggaca tgacaccggc tgtactaatc
    13081 ttcacaccga tcttcttgcc gattgcacaa gagctgggaa tggaccctgt acatttcggt
    13141 attatgatgg tggccaacct ttgtattggg ctgttaacac cgccggtggg tagtgcacta
    13201 tttgttggtt gttctatctc aggtgtgaag attcaacatt taatcaaacc attgctgcca
    13261 ttttatgctg cattgctagt agcgttgatg atgatcactt acattccgca aatatccttg
    13321 ttcattccac agctactagg gctaatgtaa aaaataattt gccggataac tttttatatt
    13381 atccggcaaa ggcacttaac atgagttact taatcacccc attgcgccgc agttgttcgg
    13441 tatctttttt ccagcgttca ttagcctggt tgatatcgtc aattttcatc aaaatcgcga
    13501 cttttttact gtttatttct gaaactttca tcgttaacgg atattgccct ttttcgagcg
    13561 ttatccagtc atcctgtaat ttacggctaa gtttttgtga ttgcagaaaa gtctgtcgcc
    13621 gttgttcgtt gatatcaaaa tctcgtttct gctgaattgg cacttcataa cactcttcac
    13681 tctgtttatg acatgccgca atcgccggtt gataaacctc tttgtcatat ttaaccgcga
    13741 acgcctcgct atcgttctgt ttattaaaat catcacgctg ttggtgaatt tttttaaagg
    13801 cgtcttcgcg ggtcatttgc ttaccgtttg catcttttcc ccaatataca tccgctgctt
    13861 tctgcgccgc caccatttgc ttatcaagcg cttttacatc ctttttcagc gcaggaatcg
    13921 agttattctg gttattcaat gcagactcaa gctgactaaa gcgataattg aaatcttcgc
    13981 tattcacgat cagatattta ttattggttt tgatatcgtc aaccacccgc ccacgatcgc
    14041 tggctgccgc ctgaaaagag taaaagttca ccgaccagcc agacgcgggc gttcctttac
    14101 tggttaacat cgccgagaat ttcaccggtt tatctttcgt ccaggtctgt tcgagcagct
    14161 cgtaatctgc caactgaccg acccaggtat agagatcgtc actggaagag acatcgcctt
    14221 ccgctgacca ggtggcctga ttacccttag catcaagatt ttttaaggtg atgacatcaa
    14281 gcttgagaat gccgtgatac tgctttttga attgatcttt taagatattt tcagttggcg
    14341 gttcaccatc tgcccggaca ttaccagtca tcaacataag cagcgccgta ccaacaatcc
    14401 attgcttttt cacccgaaaa ccctcgcgcg gaataattta ttccggtgat tatcattagg
    14461 gtaataaaaa ttggcaatgg acgtgagtct gaagtgaata agccccggca cgataccggg
    14521 gcgaagtgat tagtactgat tgaagatctg ctggatctgc tgcggatctt tagtttgcgt
    14581 cagagccagt tgcagcagaa cgcgcgcttt ttgcgggttc aacgtgccag aggcgacgaa
    14641 gccgtatttc gcatcatcca cttcggcatc ctgagtggta gcgcccgtcg gtacgcggga
    14701 agaacgcact actgcagtgc cgtttttcgc agcggttgca agggtgtcaa atacggtttt
    14761 atacaggttg ccgttaccca cgccagcgct aacgatgcca tcatagcccg catctaccag
    14821 tgctttagcc ggaagatcgg atgcgttagc gtagttataa acaatgccga ctttcggcag
    14881 ttcattcagc ttagagacat cgaacggcgt gtcgctggtg tgcttacgtg ccggggtacg
    14941 ttggtagtca atcttaccgt tgtgaatgta acccagagga ccgtagttaa cagacttgaa
    15001 ggtcgctacg tcggtggtgt tggttttggt gacatcacgg ccatcaagca cggtgtcgtt
    15061 catcactacc agtacgccac gattagcgga ggctttatca gctgcggtca ctaccgcgtt
    15121 atacaggttg aatggaccgt ctgcgctcat agacgtggac gggcgcattg caccgaccat
    15181 caccaccggt ttgtcgcatt tcaccgtcag gtcgaggaag taagcggttt cttccatcgt
    15241 gtcggtaccg tgggtaatga cgaagccgtc agttttatcg cagtcggtgt taattttttt
    15301 cgccagtgtc agccagacat catcgttcat gtcctgggag ccaatattca ctacctgctc
    15361 gcctttaacg ttcgcaatgt ccttcagttg cggcaccgca ttaaccagat tttctacgcc
    15421 aactttaccc gctgtgtagt tagatttggt tgcggagtca ccaccaccgg caatggtccc
    15481 gccggttgct aaaatggtga tattgggtaa tgccaatgct gcaccactaa aacccataac
    15541 cagtgcggca agtgccgtct ttttgaaaaa ctccatttca ttcctccagt tacgtgaacg
    15601 ctacgcatta tcccttagct ttgcatgggg aatttgacgt taaacaattt acaacgtgaa
    15661 tatattttgg agatctacaa agttagaggc aggtaacaaa acgaagaatt aaacggcata
    15721 aaaaagtatt atgccgtctt aaaatagagg attattttaa attcccgacc agggctttgc
    15781 ggctatcttc cagagtcaca acgcggctac aaacatcttt gccaaactgc tggaaatctt
    15841 tttcctgctt tttccactcg gtttggattg aggattgcag gccgcccagg cttcccagca
    15901 cgttctgtaa tgggttaccg ccgcttttca gcaccgcttt cgcgcccatt tcattaatgc
    15961 tgtcctgtaa aattccgccc attgcctgat tcactaattg ctggccttcg gcacgaacct
    16021 gatcaatggc tttatagtga aacgtcaggc catcgctgcg cgtttcgata atgcggttca
    16081 tctgctcttt cagctgcgca tcaagtttgg tcagacggct gcgcattttg ctgctttcgc
    16141 ccatctcctg aacgataatt ttatccagcg caatacgggc tttctcgacg cggcttttcg
    16201 cgccttcatc aatccacggc agggtgctac gtagttcagc ctgataatcc ttcgcctgct
    16261 cgcgctgggc ggcattcagg gaatattgct taccgttata catcacgttg ccgtctggcg
    16321 tgatcaccag attgccgttt tcgcccttca cctgcacggt ttgcgggctg acaatcacat
    16381 cgtcatgcgg cgtgacgctg cactggtagt cggcgtgagc ggtcattgcc gtcactgaaa
    16441 gtgctgccgc cagcagcatt ttgcgcatca tagtcttccc tcaagaacaa atcaggccag
    16501 catttgctgg ccccagattg ataacaaagt gcgcgttttc catgccggat gcggcgtaaa
    16561 cgccttatcc ggcctactcg atcgtgcatc tttgtaggcc tgataagcgt agcacatcag
    16621 gcagttttgc atttgtcatc gcccgtatgc tttcttagtc ccaccaaacg tcgaaaagtt
    16681 cgctggtgcg tacctcttcc agtttgcgtt cttccagcca cttacgcaca accgcctgat
    16741 gctcttcggt gcatttgccg atttcctgca tgcagatcaa accttcccag gccagatatc
    16801 cgctgccgtc aaaggccagt ttgttcggtt cgataacctc gttaataaag tcatcaacgg
    16861 ttttatcaat ctgttcttcc gatgtacctt ccgggaatcg ccatgccacc gaaaatccta
    16921 attcctggaa ttcgtcgatg tgcatttttt tacgcagacg acggctacgg ttctttgcca
    16981 ttatttcacc ctctcgaaca ttaagtccca taccccgtga ccaagacgat gaccacgttg
    17041 ttcaaatttc gtcaccggac gtgatgccgg acgcggtacg taatcattgc tctctgacag
    17101 gtttttataa ccgtcaatag aagacatcac ttcaagcata tgttccgcat aaggttccca
    17161 gtcggtcgcc atatggaata tgccccccag ctgcagtttg ctttttacca gctcggcaaa
    17221 cggcacctga acgatacggc gtttattatg gcgcgctttg tgccacgggt cagggaaaaa
    17281 gagctgcacc atgcgcaatg aattgtcagg aatcatttta tgcagcactt caaccgcatc
    17341 gtgacacatc acgcgcaggt tgcttaagcc ctcttcatgc gcagaagaca ggcacgcacc
    17401 aacacccggt gaatgcactt caatgccgag gaagtcctgc tcggggcgat ctttagccat
    17461 tgccaccagc gacgcgccca tgccaaaacc aatctcaagc gtcaccggcg cttcacggcc
    17521 aaaaagcgca gggaaatcca gcatatcttc gctgaactca acgcccatca ccggccagta
    17581 gttttccagc gcatgttcct ggcctttggt cagtcgcccc tggcggcgca caaaactacg
    17641 gatacggcgc agcgggcggc cgttttcatc aaattccggt gagatgacgt cgtttttcat
    17701 aaagatttag tcgcttgtga aagtgttctg aaaacgggca ttatccaaag ttagttgccg
    17761 gatgcaagca tgataaggcc gtggctgcgg aaagttccgg tttacaccct gccgtcgctg
    17821 tgctgcaatc ttgcccccaa caacagtgaa ttcggtgacc atgcaagcgt cgcaattttc
    17881 agcccaggtt ctggactggt acgataaata cgggcggaaa acgctgccct ggcaaattga
    17941 caagacgccc tacaaagtat ggctctcaga agtgatgttg caacaaactc aggttgcgac
    18001 cgttatcccc tattttgaac gctttatggc acgcttccca acggtgaccg atctcgccag
    18061 tgcgccgctc gacgaagttc tccacttgtg gaccgggctt ggctattacg cccgcgcgcg
    18121 caacctacat aaagctgcac aacaagtggc ggccttacac ggcggtaaat tcccggaaac
    18181 ctttgaggaa gttgcagcac tgccgggcgt cgggcgttcc accgcaggcg cgattctctc
    18241 gctttctctg ggtaagcact ttccgattct cgacggtaac gtcaaacgcg tgctggcgcg
    18301 ctgctatgct gtaagcggct ggcccgggaa aaaagaggtc gagaataaac tatggagttt
    18361 gagcgagcag gtgacgcccg cggttggcgt ggaacggttt aaccaggcga tgatggattt
    18421 gggcgcgatg atttgtacgc gctctaagcc gaaatgttcg ctctgtccgc tacaaaacgg
    18481 atgtattgcc gccgccaaca atagctggtc gctttatccg ggcaaaaaac cgaaacagac
    18541 gctgccagag cgcaccggct actttttgct gttacagcac gaagatgaag tattgctggc
    18601 gcagcgtccg ccgagcggat tgtggggcgg tttatactgt ttcccgcagt ttgccgatga
    18661 agaaagtttg cggcagtggc tggcgcaacg acagatttct gccgataacc tgacgcagct
    18721 gaccgcgttt cggcatacct tcagccattt ccacttagat attgtgccta tgtggcttcc
    18781 cgtgtcgtca ttcaccggct gcatggatga agacaatgcg ctctggtata acttagcgca
    18841 accgccgtca gttggcctgg cggctcccgt ggagcgttta ttacagcagt tacgcactgg
    18901 cgcgccggtt tagcgcgtga gtcgataaag aggatgattt atgagcagaa cgattttttg
    18961 tactttcctg caacgtgaag cagaaggtca ggattttcag ctgtaccccg gcgaactggg
    19021 aaaacgcatc tataacgaga tctccaaaga agcctgggcg cagtggcagc acaaacaaac
    19081 catgctgatt aatgaaaaga aactcaacat gatgaatgcc gagcaccgca agttgctgga
    19141 gcaggagatg gtcaacttcc tgttcgaggg taaagaggtg catatcgagg gctatacgcc
    19201 ggaagataaa aaataaaaac agtgccggag cacgcctccg gcaacttgca taaaaacaaa
    19261 cacaacacgc acccggaatg atgaaaaaat atctcgcgct ggctttgatt gcgccgttgc
    19321 tcatctcctg ttcgacgacc aaaaaaggcg atacctataa cgaagcctgg gtcaaagata
    19381 ccaacggttt tgatattctg atggggcaat ttgcccacaa tattgagaac atctggggct
    19441 tcaaagaggt ggtgatcgcc ggtcctaagg actacgtgaa atacaccgat caatatcaga
    19501 cccgcagcca catcaacttc gatgacggta cgattactat cgaaaccatc gccgggacag
    19561 aacctgccgc acatctgcgc cgggcaatta tcaaaacgtt gctgatgggg gacgatccga
    19621 gttcggtcga tctctattcc gacgttgatg atattacgat ttcgaaagaa cctttccttt
    19681 acggtcaggt ggtggacaac accgggcagc cgattcgctg ggaaggtcgc gcgagcaact
    19741 tcgcggatta tctgctgaaa aaccgtctga agagccgcag caacgggctg cgtatcatct
    19801 acagcgtcac cattaacatg gtgccgaacc accttgataa acgtgcgcac aaatatctcg
    19861 gtatggtccg ccaggcgtca cgcaaatatg gcgttgatga gtcgctgatt ctggcaatta
    19921 tgcagactga atcttccttt aacccgtatg cggtcagccg ttccgatgcg ctgggattaa
    19981 tgcaggtggt acaacatacc gccgggaaag atgtgttccg ctcgcagggg aaatccggca
    20041 cgccgagccg cagtttcttg tttgatcctg ccagcaatat tgataccggc accgcgtatc
    20101 tggcgatgct gaacaacgtt tatctcggcg gaattgataa cccaacatcg cggcgttatg
    20161 ccgtcatcac cgcctataac ggcggcgcag gcagcgtgct acgagtcttt tcgaatgata
    20221 agattcaggc tgccaatatt attaacacca tgacgccggg cgatgtttat cagacgctga
    20281 cgacccgcca tccctctgcg gaatctcgcc gttatcttta taaagtgaat accgcgcaaa
    20341 aatcttaccg ccgccgataa ttccactaac cgcccctgac gatgctcagg ggtaaaaatg
    20401 ttatccacat cacaatttcg ttttgcaaat tgggaatgtt tgcaattatt tgccacaggt
    20461 aacaaaaaac caatccgcga agttgataga atcgcatcat ctcgcacggt caaatgtgct
    20521 ttttcaaaca ctcatccgca tcacgatgtg aggaaattaa catgaatctt aagctgcagc
    20581 tgaaaatcct ctcttttctg cagttctgtc tgtggggaag ttggctgacg accctcggct
    20641 cctatatgtt tgttaccctg aagtttgacg gtgcttctat cggcgcagtt tatagctcac
    20701 tgggtatcgc cgcggtcttt atgcctgcgc tgctggggat tgtggccgac aaatggttaa
    20761 gtgcgaaatg ggtctatgcc atttgccaca ccattggcgc tatcacgctg ttcatggcgg
    20821 cagaggtcac tacgccggag gcgatgttcc ttgtgatatt gattaactcg tttgcttata
    20881 tgccaacgct tgggttaatc aacaccatct cttactatcg cctgcaaaat gccgggatgg
    20941 atatcgttac tgacttcccg ccaatccgta tctggggcac catcggcttt atcatggcaa
    21001 tgtgggtggt gagcctgtcc ggcttcgaat taagccacat gcagctgtat attggcgcag
    21061 ccctttccgc cgttctggtt ctgtttaccc tgactctgcc gcatattccg gttgctaaac
    21121 agcaagcgaa tcagagctgg acaaccctgc tgggcctcga tgcattcgct ctgtttaaaa
    21181 acaagcgtat ggcaatcttc ttcatcttct caatgctgct gggcgcggaa ctgcagatta
    21241 ccaacatgtt cggtaatacc ttcctgcaca gcttcgacaa agatccgatg tttgccagca
    21301 gcttcatcgt gcagcatgcg tcaatcatca tgtcgatttc gcagatctct gaaaccctgt
    21361 tcattctgac catcccgttc ttcttaagcc gctacggtat taagaacgta atgatgatca
    21421 gtattgtggc gtggatcctg cgttttgctc tgtttgctta cggcgacccg acgccgttcg
    21481 gtactgtact gctggtactg tcgatgatcg tttacggttg cgcattcgac ttcttcaaca
    21541 tctctggttc ggtgtttgtc gaaaaagaag ttagcccggc aattcgcgcc agtgcgcagg
    21601 ggatgttcct gatgatgact aacggcttcg gctgtatcct cggcggcatc gtgagcggta
    21661 aagtggttga gatgtacacc caaaacggca ttaccgactg gcagaccgta tggctgattt
    21721 tcgcaggtta ctccgtggtt ctggccttcg cgttcatggc gatgttcaaa tataaacacg
    21781 ttcgtgtccc gacaggtaca cagacggtta gccactaatt acgcaaagaa aaacgggtcg
    21841 ccagaaggtg acccgttttt ttattcttac ttcaacacat aaccgtacaa ccgtttcatg
    21901 ccatccgcat cggtttcgct atagacacct tgcaactccg gcgaaaatcc cggcaacaaa
    21961 ttcacgcctt cttccagcgc aaggaaataa cgttgaaccg ccccacccca gacttccccg
    22021 ggtaccacgc aaagcacgcc aggtggataa ggcaacgccc cttctgccgc aattcgccct
    22081 tcggcatcac gaatccgcac caactccact tcaccgcgaa tataagcgct atgcgcatcc
    22141 tggggattca tcaccactga cgggaaactc tgctggcgga acatcgcttt ttgtaggtct
    22201 ttgacgtcga aactgacata cagatcgtgc atctcctgac acaactgacg cagggtgtag
    22261 tcgcgatagc gcaccggata tttgttataa acgctcggca acacctcagc cagcggcgag
    22321 tcatcctcaa tatgctgttc aaactgcgcc agcatcgcca ccagttgtgc cagcttctcg
    22381 tggctttccg ccggagttaa taagaacaga atggagttga gatcgcactt ctccggcaca
    22441 atgccgttct cacgcagata gtgcgccaga atcgtcgccg gaacgccaaa gtcgctatat
    22501 tcgccggttt cagcatcgat acctggtgta gtgagtaaca gcttgcacgg atcaacaaaa
    22561 tactgatccg cggcatatcc ttcaaagccg tgccacttcg ccccaggctc aaaactgaaa
    22621 aaacggcggt cgctggctaa cactgatgtc ggataatcct gccacaattt gccatcaaca
    22681 acaggcggga taaacgggcg gaacagctta cagcgcgcaa gaatagcctt gcgcgcttca
    22741 atccctatct caacacactc agcccacagc cgacgcccac tctccccttc atgaattttg
    22801 gcgttaacgt ccagcgcggc gaacagcgga tagaacgggc tggtagaagc atggagcata
    22861 aaggcattat tcaaccgctt atgcgggcaa aaacgcgcct gtccacggat atggttatct
    22921 tttttatgga tctgcgacgt ctgtgagaat cccgcctgct gtttgtgcac cgactgggtc
    22981 acaaagatcc ccggatcgtt ttcgttaagt tctaacagca gcggcgagct atccgccatc
    23041 atcgggataa actgttcgta accgacccac gcggaatcaa acagaatgta atcacacaga
    23101 tgcccaacgg tatcgatcac ctgacgggcg ttatagacag tgccgtcata ggttcccagc
    23161 tgaataatcg ccaggcgaaa cgggcgcggc aggtcggctt tttctggcac aacgtcgcga
    23221 atttgctggc gcagatactc ttcattaaaa cagtgcgcat caataccgcc aatgaaacca
    23281 aacgggttac gtgaagcttc cagatagacc ggcgtcgccc ccgcctgaat caacgcgccg
    23341 tgatgattcg acttatggtt gttacggtcg aagagcacca gatcgccacg cgttaacagc
    23401 gcattcgtca ccactttatt cgctgccgat gtgccgttca gcacaaaata ggttttatcg
    23461 gcatgaaaga ctttggctgc gaatttctgc gcatctttcg ccgatccttc atgaataagc
    23521 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagatgtt ctcgccaaag
    23581 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaga acgcaccatg ttgatgtcca
    23641 gggcaagcaa aggtgctatt acccatctca acgtactgca tcagcgtgtc ataaaacggt
    23701 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
    23761 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
    23821 aacaccggta gatgaaaacc ggtgctctta agcaacgcaa gaatgccact gcgactatcc
    23881 gcagcggtaa tgacgactgc cgcgacgtcc gtaaaatcag tatctcccaa cgccaccacg
    23941 cggcgatgag tagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
    24001 ataagcgcaa aacccgttta ggggaagtaa gaataccgga caaggtggaa aaccctgccc
    24061 catgagatat gggtcaaact ggtcaccagc tccgaccacc agacatcagt aaaagcagaa
    24121 acgctctgat tttactgttg tcctgcagtg agcgtgcgtt aacttcaccg catgagcagt
    24181 agcataaaaa gagaagagtt tcgcgcgaaa cggcgataag cgagagaatg taaggaggtg
    24241 gcgtgcatcg ggcaaactcc gtacagagag gagaaaattc gcgcaatcat ggcacctttc
    24301 gctaaagcgt gcaagccagg actttgcgaa caaacaagcc atcgacggcg ccaaaagttc
    24361 ataataagaa aatcaaacaa tacagctaac aggaactctt gtggtcatcg gcccttttat
    24421 caacgcaagt gcagtcttac tgggtggcgt tttcggcgca ctccttagcc aacgcttacc
    24481 ggaacgtatc cgcgtctcca tgacatcaat ttttggtctg gcatcgctgg ggattggtat
    24541 tttactggtg gtgaaatgcg ccaaccttcc ggcgatggtt ttagcaacct tactcggagc
    24601 attaatcggc gaaatttgtc tgctggaaaa aggcgtcaat acagtggtcg ccaaagcgca
    24661 taatctgttt caccactcgc gtaagaagcc agcgcatgaa tcttttattc agaattttgt
    24721 cgcgattatt gtcctgtttt gcgccagcgg caccggaatc ttcggggcga tgaacgaagg
    24781 gatgaccggc gatccgagta ttttaatcgc caagtcattt cttgatttct ttacggcgat
    24841 gatcttcgcc tgctcgctgg gtattgcggt gtcggtgatt agcattccat tactgatcat
    24901 ccagttaaca ctggcgtggg ctgccgcgct gatattaccg ctgaccacac cgtcgatgat
    24961 ggcagacttc agcgccgtag gcggtttatt gctgctggca accggattac gcatctgtgg
    25021 cattaaaatg ttcccagtgg tcaacatgct tccagcactc cttctggcaa tgccgctttc
    25081 cgccgcctgg accgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag
    25141 attgtgcagt ctgcactaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt
    25201 aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt
    25261 ccttggttcg attccgagtc cgggcaccac tttcacttcc agattcatct cccgaactcc
    25321 acgaaactca agtataatca catcgttaca agcaatctga acatcctgaa ctccaccgaa
    25381 ctctactgaa ctctctctca atcaagcgtt cattgggggc accggtgggg gctcattgtg
    25441 ttcagtcagt ttcaggagcc cccatatggc acttacagcc agacaggtcg aaacagccag
    25501 acctaaagaa aaagactata aactctctga cgaacgtggt ttatatctgc tggtaaaaac
    25561 cacgggtgcc cgctactggc ggcttaaata ccggatagca ggaaaagaga aaaaactggc
    25621 cctcggcgtc tatcccgacg tctcccttgc tgaagccaga atcaaacgcg acgatgcccg
    25681 aaaaattatc tccgaaggtg gcgacccggg cgaaaagaag cgaaaggaaa aactcactca
    25741 gaaaatctct gccaccaata cgttccatgc ccttgctacg gaatggcatc agcataaatc
    25801 tttgtcatgg tctgaaagtt acgccagaag cgtactggag gctctggata aagatatttt
    25861 cccgtatctg ggcaaacgaa gcgttactga tatcctcccg ctggaaatgc tggaaattct
    25921 gcgccgcata gaaaaacgtg gctcgctgga aaaacttcgt aaggtgcgtc aatactgtaa
    25981 tcagattttt cgttatgcca tcgccaccgg acgagccact gtcaatccgg catctgaact
    26041 gaccagtacg ctggcggcgc caaaagctgc acatttcccc cacctgagag cagatgagct
    26101 ccctgttttt ctccggaagc tcgctgaata tcatggcagt cctgttaccc gcatggcgac
    26161 aaatctgctg cttctgacag gcctcagaac gattgaacta cggtccgctg aatggtcaga
    26221 aattgatttt gataatgccc tgtggacaat ccctgaaagc cgcatgaaaa tgcgacgtaa
    26281 acatgtcgta ccactgtcac gacaggccac tgacattctg ctgcagctca aaactttctc
    26341 cggacaatac cggctggttt tcccgggacg ttgtgatatc aacaagccaa tgagcgaagc
    26401 cagcatcaat atggtgctca aacgtatcgg ttacgatggc agggcaaccg gtcatggttt
    26461 tcgtcacacc atgagtacca ttctgcacga acagggcttt aattctgcct ggattgaaat
    26521 gcagttagct catgtggata aaaattccat caggggtacc tataatcatg ccctgtatct
    26581 cgatggtcgc cgtgaaatga tgcaatggta cgctgactac attgattcac tttccatcca
    26641 ggagagttaa ttacactttg tcctgaaatt attctcacaa tcaataacct ttccctctga
    26701 tctgtaagat cagagggatt ccgttaactt acaataagtg atattggttg tctggagatt
    26761 tagggggacg gtctactata ataattagca ggagataata tgtcacttaa ggcgattgcc
    26821 actgcactcg gagtttctgt caccactgtc agtcgggctc ttggaggcta tgcagatgtg
    26881 tcagcttcca cccgtgcacg ggtggaagct gaagctcgtc gacgcggtta ccgtcctaat
    26941 actcaggcaa gaagactcaa aaccggtaaa accgatgctg tcggtctggt ttatcctgga
    27001 cgtgatgtgc catttaacag tggtgttttt atggatatgg tcagttgcat cagccgggaa
    27061 cttgctcacc atgatattga cttactactg atagctgatg atgagcatgc cgattgccac
    27121 agctatatgc ggcttgttga aagtcgcagg gttgatgctc tcatcattgc acacactctg
    27181 gatgacgatc cccgtatcac acaccttcat aaagcaggta ttccatttct ggctcttggt
    27241 cgggtaccat ctggtctgcc ctgtgcgtgg tttgactttg ataatcatgc cgggactgga
    27301 caggcaaccc agaaactgat tgctttggga cataaacgta ttgcattgtt gagtgaagac
    27361 acttcacata gctatgttgt tgcaagacgt cagggatggc ttgatgcact gaatgagcat
    27421 gaactggaag atacatggct gcggctggtt tctcccacac ggcgagcagg atatcaggcc
    27481 gtgatggagt taatgtcact accgatacca ccaacagcta ttattactga caatgacctg
    27541 agtggagatg gtgctgccat ggcactgcag ttgagcggtc gtctttcagg aaaagatgcg
    27601 gtatctctgg ttgtatatga tggtttgcca caggacagta ttattgagct ggatgtggct
    27661 gcagttattc agtcaacacg aagcctcgtt ggtcgccaga tttccgagat gatatatcag
    27721 ataattactg attcatcatc aaaaccacta caggttgtct ggaacccggt attttatccg
    27781 ggaaagacga ttcatcctcc ttgctcctga tttttttatc cagctcacat atccgaaacg
    27841 ttttggttga tacccgaaac gtttcggatc aacagtaagg catccctgag aaaggagaat
    27901 gtcttatggt ttcaaagtac tgcagactga gcagtcctcg ctctgattta attattaaaa
    27961 cccgtccaca tgcagaaatt atctggtggg gctctgcact gaaacatttc tcaccggatg
    28021 actgtgccag cctggaaaga ccggttgcga atggtcgtct ggatattgat acgccactaa
    28081 cactgatggc tgaaaatgcc ctgggacttt ttagctctcc gggactggaa ggacacagga
    28141 atgggctgga tgcatctcct gttttttata cagttgacgt ggaacatacc gaaaacactc
    28201 tgagacttac cagtgaagat tcggtagccg gcctgcgtct ggtcagcgag ctggtgatga
    28261 cgccatcagg aattctgaaa gttcgtcatg cactgaccaa cctcagagag ggggactggc
    28321 agataaatcg tttcgccatc actttacctt tagcggaacg tgcggaagaa gtcatggctt
    28381 ttcacggacg ctggactcgt gaatttcagc cgcacagggt acgtctcact catgatgctt
    28441 ttgttctgga aaatcgcaga gggcggacat ctcatgagca ttttccggcg ctgattgtcg
    28501 gcacgccagg cttctcggaa caacagggag aggtgtgggc tgtgcatctg gggtggagtg
    28561 gaaatcaccg catgagatgt gaggcaaaaa ctgacggcag gcgttacgtt caggctgagg
    28621 ctctgtggat gccgggtgag aaggctctca ggaagaatga aaccctgtac accccgtggc
    28681 tatatgcctg ccactctgcg gatggcctga atggaatgag tcagcaatac catcgttttt
    28741 tgcgtgatga aattatccgt ttccctgagc aaaaaccccg ccctgtacat ctcaatacct
    28801 gggaaggtat ttatttcaat cacaatcctg attacatcat gcagatggct gagcgtgcag
    28861 cagcactggg cgttgaacgt ttcattattg atgatggctg gtttaaagga cgtaacgatg
    28921 accgcgcggc tctgggcgac tggtataccg atgaacagaa atacccgaac gggctgatgc
    28981 cggttattaa acatgtgaaa tctctcggta tggaatttgg catatgggtt gagccggaaa
    29041 tgattaaccc ggattctgac ctgtttcgtc ttcatcctga ctgggtattg tcaatgcctg
    29101 gatattccca gccaaccgga agatatcagt atgttcttaa cctgaatatt ccagaggcct
    29161 ttgcttacat ttatgaacgt ttcttatggt tactgggaga acatccggtt gattatgtga
    29221 aatgggacat gaatcgtgag cttgtacagg cagggcatga aggccgtgcg gcagcagatg
    29281 cacagacccg tcagttctat cgattgcttg atctcctccg tgaacgtttt ccacatgttg
    29341 agtttgagtc ctgtgcttcc ggtggggggc gtattgactt cgaagtcctg aaacgcacac
    29401 accggttctg ggcatctgac aataatgatg ccctggagcg ctgcaccata caacgtggca
    29461 tgagttactt tttccctcct gaggtgatgg gggcgcatat tggccatcgc cgctgccatg
    29521 caactttccg gcagcacagc atcgcttttc gtgggctgac ggcattgttc ggccatatgg
    29581 ggctggagct ggatccggtg gccgcagatg cgaaggaatc tgacggttat cgccggtatg
    29641 ccttgctcta taaagaatgg cgacaactga ttcatacagg agttctctgg cgtgtggata
    29701 tgccagatcc ttcgatacag gttcagggag tcgtcagccc tgatcagtct caggcacttt
    29761 ttatgatcag ccagcttgca atgccggatt acaccttacc aggcatactt cgttttcccg
    29821 gtctggcggc ggaagtgcgt taccggcttc gggttattga tcacccggac ctccaggtgg
    29881 ttggtgaagg cggtcatacc atgcgcaaat tacctgtctg gatgaatcag agccttgagg
    29941 ccagtggtga atggctggca cagggaggga ttcagctccc cgtactggat ccggagagtg
    30001 cgattttgat agcacttgaa agagctgtgt gaagagaggt acggagcctc agctccgtcc
    30061 tgtttctcaa tattatcccg tgagggtaag aataatgaat tctgcaagta cgcataaaaa
    30121 tcccgatttc tggattttcg gtctgttctt ctttctctac tttttcatca tggcaacctg
    30181 ttttccgttt ttgcctgtat ggctgtccga tgtggtcgga ctgagtaaaa cggatacagg
    30241 tatagtcttt tcatgtcttt ctctgtttgc catcagtttc cagccattgc ttggggtcat
    30301 atcagatcgc ctgggactga aaaaaaacct gatctggagt atcagcctgt tacttgtatt
    30361 tttcgccccc ttttttttat atgtatttgc gcccctgctg cgcttcaata tctgggcagg
    30421 cgcactgact ggcggtgtct ttattggttt tgtgttttct gcaggtgccg gagctattga
    30481 agcttatata gagcgggtca gtcgcagtcg tggatttgaa tacggtaaag cgaggatgtt
    30541 cgggtgtctg ggctgggcgt tatgtgcggc tatggctgga atgcttttta atgtcgatcc
    30601 ttctctggtt ttctggatgg ggtcaggaag cgcattattg ttgcttcttc tgttgtttct
    30661 ggcgcgcccc agtaccagcc agacggcaat ggttatgaat acactgggtg ccaattcttc
    30721 cctgatttcg accagaatgg tcttcagcct gtttcgcatg cgtcagatgt ggatgtttgt
    30781 tctctacacg attggtgtgg cctgtgtcta tgatgtattt gatcagcagt ttgccacatt
    30841 ttttcgttca ttctttgaca ctcctcaggc aggaataaaa gcattcggat ttgctaccac
    30901 tgcgggggag atttgtaatg ccattatcat gttctgtaca ccatggataa ttcatcgcat
    30961 tggtgccaaa aataccctgc ttgttgcggg gggaattatg accatccgca ttaccggttc
    31021 tgcttttgcc accaccgcga cagaagtggt gattctgaaa atgcttcacg ctcttgaagt
    31081 tccatttttg ctggttgggg cgttcaaata tattacggca gtgtttgaca cccgactgtc
    31141 agcgaccgtt tatttaatag gttttcagtt ttccaaacaa cttgctgcaa tacttctctc
    31201 tacctttgcc ggccacctgt atgatcgtat gggattccag aatacgtatt ttgtgctcgg
    31261 gatgattgct ctgactgtta ccgtgatatc agttttcacg ctgagttctt cccgcgggag
    31321 cgtacaccct tctgtagaaa aagcccctgc agcgcattcg gagattaact gatgaaacag
    31381 cgtcttgctt tggcacagtc tgcccttgaa aaactttgcg cacgtcgtgg taatgcctgg
    31441 tacccgattt ttcatctggc tccacctgcc ggctggatga atgatccaaa tggccttatt
    31501 tacttcaatg ggcgttacca tgcgttcttc cagcatcatc ctgcaagcgc atatcagggg
    31561 ccaatgcact gggggcatgc caccagtacc gacatgttgc actggcaaca cgaacctatc
    31621 gcgctggcac ccggagataa atatgatcgt gatggctgtt tttcagggag tgccgtggat
    31681 gatgatggcg tgctatcact tatttatact ggtcatattt gtctcgatga tcgtggtaat
    31741 gacagcatta tccgtgaagt acagtgtctg gctacaagtc atgatggtat tcactttgag
    31801 aagcagggct gtgtgctgac acctccggaa ggtataatgc atttccgtga tcccaaagtc
    31861 tggcacgaag acggctcctg gtggatggtc attggtgccc gggacgcttc tgacaatggg
    31921 caagttctgt tgtatcgcgg gacatctttg cgggactggc atctggagca tgttcttgct
    31981 cattccgcag ccggaaaaag ttatatgtgg gaatgccccg atttcttcag gtgtggtaat
    32041 tttcactggc tgatgttctc accacagggg atgccccctt ccggttatcg gttccgtaac
    32101 ctttttcaga gcggtgtgtt ggcagggagc tggaagcctg gttctgtctt tgcgctgaaa
    32161 gggggatttg aagagctgga ttatggtcat gacttttatg ctccacagtc catgctggct
    32221 gaggacggca ggcgtatcat tatggcatgg atgaatatgt gggattcacc cgtgcccacc
    32281 cgcagtgaag cctgggcagg atgtctgacg ctgcccagag aggtttttga gcgcgatggc
    32341 cggctgtgcc agcgacctgt gcgtgaagtc gaatctctgc gcaaaaaatg ccagccatta
    32401 tcccctgtaa ggttacaggg tttgcaatta ctgaccgaaa atgtacaggc cgcagaatta
    32461 ttggtgacgt ggcatacggt tgacagtcat gcggagcact atggcgtccg ccttggagac
    32521 ggtctgcgac tttatgtgga taatcaggcc gggcgactgg tactgtggcg ctattaccct
    32581 gaggaagggt tggatggtta ccgcagtgtt gaacttcctg atacagaata tctgactctt
    32641 cgtattttct tggatcgttc atctgttgaa gtgtttgtta acgatggtga ggcaacctta
    32701 tcaagtcgta tttatccgca agcggactcg agacaattat cgttatatgc cgctcatggc
    32761 gatgcgatat taactgatgg cactttatgg atgctgacct gagacatatc gatatcagta
    32821 ttttttctga cattatctgt cattactttg ccagtacaca ttaattatct ttgctgtgtg
    32881 cttttggtaa tgtgttgcga ttatatatac cagtctggtg aagaatatga atggtatttt
    32941 gtgttgatac agagaatata agagtaccca catttgtttt tatttaaggg ggtaaaatga
    33001 ataagttact tttgggttta tctctttttt tattactgga atacaatgcg tgtgcagcaa
    33061 aaacaggggg gctaacgctt gaacaaagaa tggcattgct ggaagaacgc ctggaagctg
    33121 cagaaaaaag ggcggaaaaa gcagaaagaa tgttaaagag ttttgatata gaacaacatt
    33181 ctgaaatacg tcaaattagt tcagagcaga ataaaaaaga tgctaacagc tatgcagtag
    33241 ttgagtcaac aaaagaaaaa acatcttctc caggttttct gctctctggt tacaatgatt
    33301 tgaagtttta tggtgatgtg gagtttaata tagatgctgc cagtaaaccc ggtcagttag
    33361 taatgataag ttccggggcg aacagtgagt cagtgaatga acggtgggac cttaatggcc
    33421 gtattctatt aggttttgac ggtacccgta agcttgataa tggttatttc gctggatttt
    33481 cagcacaacc actggcggat atgcacggtt cagtgaatat tgatgatgca ttattctttt
    33541 ttggaaaaga tgacgagtgg aaagtgaaag tcggtcgttt tgaagcttac gatatgttcc
    33601 ctctaaatca ggatactttc attgagtatt ccggtaatac agctaatgat atttatgctg
    33661 atggccgtgg ttatatctat atgatgaaag agggacgcgg tcgttctgac gctggtggta
    33721 atttcctcat cagtaaacaa ctcgataact ggtattttga gttaaacacg ttacttgaag
    33781 acggaacatc tttatataat aacggtaatt atcatggacg atatatggag cagcagaaaa
    33841 atgtcgctta tctgcgcccg gtaattgcct ggtcgccaac ggaagaattc acagtctccg
    33901 cagcgatgga agcgaacgtg gtaaaaaatg cttatggcta taccgataat aaggggaatt
    33961 ttgtcgatca gtccaatcgt tccggttatg gtattagcat gacatggaac ggtctgaaaa
    34021 cagatccgga aaatggcatc gtggttactc ttaataccgc ctatttggat gccagtaatg
    34081 agaaagattt cactgccagt attaatgctc tgtggaaacg tttcgaactg ggttatatct
    34141 acgcacataa taagattgat gaatttagtg gtgtcgtgtg taataatggt tgctggattg
    34201 atggtgaagg aatatacaac attcacacca ttcatgcatc ttatcagttt gctaatgtga
    34261 tggatatgga gaactttaat atttacctcg gggcttatta ctcaattctg gatagtaact
    34321 gtagatatag taattgtgac attaccgatg atcgttacgg tgcccgatta cgtttcaaat
    34381 actttttttg atgcatagta acaaagtatt gttccacact tacattaacg cgtttttcgg
    34441 gcgcgtcatc agcgatgcat acgaatgatc gcagtctgcc aatggtcgat ttatcttggt
    34501 tgcgcagggg cgagcaccgg gcaggtaccg gcggctgact ctgaagctct gagtcgggta
    34561 attgcgtcag gtcacgaagg actgacacgg gtattggtgg caggtgatga agtactgagt
    34621 gtggtaattt cggggatgca atgcaggagc tgagtcaggt ttgcactgag ctgaggaccc
    34681 gtagcgagcg ggttcctgct atttttcggt gtcaggggcg caagtcccga cgtcgggact
    34741 tgccgggaga ttgtcaggtc attgaccggg tgtgagcggc tcgacgtaag cggtagatat
    34801 gcccaattcc ccccgataaa ttcattacca aacacattat tcaatttgtt tttatttttc
    34861 cgctttcagt cattatcgct atcgcttcgt cggtaatgac tccaacttat tgatagtgtt
    34921 ttatgttcag ataatgcccg atgactttgt catgcagtgg atttgcccct atatttccag
    34981 acacctgtta tcacttaacc cattactggc ccgctgccgt agatattccc gtggcgagcg
    35041 ataacccagt gcactatgcg gatgccattc gttataatgc tcgaacgcct ctgcaaggtt
    35101 ctttgctgcc gttaacccgt ctggtttggg catgatactg atgtaatcac gctttatcgt
    35161 tttcacgaag ctctctgcta tgccgttact ctccggactc cgcaccgccg tgttcttcgg
    35221 ttcaagtccc aacatccggg cgaactggcg cgatttagcc ccgacatagc cccactgttc
    35281 gtccatttcc gcgcagacga tgacgtcact gcccggctgt atgcgcgagg ttaccgactg
    35341 cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgggct
    35401 gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg cgtaccgagt
    35461 tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag
    35521 cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag
    35581 ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat
    35641 cagtaagttg gcagcatcac ccgctttgaa gtgaacaata accagggtcg tttcgtattt
    35701 ccggatacct ggtttggccc attactgggg gagtttgaag aagtgcttga tgcatacgac
    35761 actgatgaaa tcagtgagac gggttatatc aacaaactca gacgccttgc tcaacaggag
    35821 cctgacttta ttgatataca cgctcatctg gcatatgcat ttctggagca gaatgccccg
    35881 cgtaaggcac tgaacgccgc tcttaaagga ctggcggcag gtaatcgtct catcccggaa
    35941 agtttcagtg gtgaaatcat ctggatgcat cccgaaaacc gcccttatct gcgcgcgctt
    36001 tatgcaacaa ttctcgcaaa tgtgcactta cagcgtcatc aggatgcggt tatgctcact
    36061 gataaaatac tggcatacaa tcctgaagat aatcagggtg cccgctggtt gttgggctca
    36121 gagctgttac gaacaggtga tcatgagcga gccttcagtg tactgaaaaa acatgctgat
    36181 gaattttcac cttactggta tgaacttggt cttctgcatt ttctgaatgg ggagcatgtt
    36241 aaggctgcca cagcttttcg tcatggcttt gctacaaaca cttacatcgc ggaaatgctg
    36301 tgcggaaatt tgcatccatt tccacttgct gtgcggcata acttctccgg ctctctggat
    36361 acggcagaag attactatgc aacatactca ccattatggg gacaatatcc ggaagcattg
    36421 ctgtttgtaa actggctcta taaccattcc tctgtgctgc atgaacgagc agaaatcatt
    36481 aaatgcgccg aaatgctgat gcaggaagat gatttcgaaa tatgcgaaag cattttaaga
    36541 cagcagaagc tgttgcggga aagaattgat gagacacttt ctgaggaaat tgtacagaaa
    36601 tgcagaaata ttaacgatga atatgtctgg ccctggatat tgccgttttc agcagcagga
    36661 atgaaacact caagcatcca gcatcagtag atattgcatt agtgtatctt tcaaacaagt
    36721 aataatttat ccaccagtaa taacacagtc ccccttcccc aggttatcgc cctgggggca
    36781 ttttctgaaa gtttccttta aaaaagaata gagtcgtaaa ttactttaac gaattattcc
    36841 tggagttcat taaagttctt tcgtaatact ttgtagtcaa acacgttatg actggcatca
    36901 aaaaccacgg atttcccctg ccagcgggta atcacctcct ccggcgtttt cggttgagcc
    36961 ggtctggtta cgcccatcca tgcattaatc agataatcat tatacgcagt ggaaaaaacg
    37021 ctgttcaccg tctggcgatc gacatgctct cccagtgtcg ggctgtacca gataaacatc
    37081 ggaacatgat aagcctgctg actgggtttt attccaccat gaaaataggc atgttctttt
    37141 gtcgggtcat actccagacc gtgatctgaa aaatacatga cggatgcccg acgcgtttcc
    37201 agcatggcga aaacctgtcc caacaaactg tcggtataat gaatggaatt atcgtaacag
    37261 acttcttctg tatccagcgg tttaaacacg gctttatctc tgggccagtt actgcaaacc
    37321 ggttcatggc tgcccgtcag atgcagaaca atcagtttcc tgctccccgg attactgttc
    37381 agtgcttcag ccaggtgtgg gagcagtaac tcatcatagc ctctgacata aattctgttt
    37441 ctggcccgca tggcgatact ggccacagcc gttccgttct gtcggaaagc ggactgcgca
    37501 ctgagccacc aggtatcgaa ccccgcctga ttcgccatgt tgataatgtt atcagggtaa
    37561 ttacggatat catgatgcag tacactatcg gcagataacg ccagtggaac agccagcgcg
    37621 gtgtaaggtg cgccactgat tgcctgagtg aacagtttca gtcgtgattt ctgtttctga
    37681 agctctggtg tggtagggcg cgaatacccg taaatggaca tgttatcagt ccttgcagat
    37741 tcaccaatca ccagaacaaa tacgtcagtg ttgttatccg taatcaccag atcgtagtga
    37801 ggtatggtat cagcaatcgt cattaatctc tgat
//
LOCUS       DAJOTB010000029        37655 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJOTB010000029 DAJOTB010000000
VERSION     DAJOTB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.029"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..1798
                     /locus_tag="OHN28_001503"
     CDS             140..1798
                     /locus_tag="OHN28_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCQ2296918.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1795..2751)
                     /locus_tag="OHN28_001504"
     CDS             complement(1795..2751)
                     /locus_tag="OHN28_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCQ2296919.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            2902..4017
                     /gene="macA"
                     /locus_tag="OHN28_001505"
     CDS             2902..4017
                     /gene="macA"
                     /locus_tag="OHN28_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCQ2296920.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4014..5960
                     /gene="macB"
                     /locus_tag="OHN28_001506"
     CDS             4014..5960
                     /gene="macB"
                     /locus_tag="OHN28_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCQ2296921.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6033..6257)
                     /gene="cspD"
                     /locus_tag="OHN28_001507"
     CDS             complement(6033..6257)
                     /gene="cspD"
                     /locus_tag="OHN28_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCQ2296922.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6580..6900
                     /gene="clpS"
                     /locus_tag="OHN28_001508"
     CDS             6580..6900
                     /gene="clpS"
                     /locus_tag="OHN28_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCQ2296923.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6931..9207
                     /gene="clpA"
                     /locus_tag="OHN28_001509"
     CDS             6931..9207
                     /gene="clpA"
                     /locus_tag="OHN28_001509"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCQ2296924.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9551..9638)
                     /locus_tag="OHN28_001510"
     tRNA            complement(9551..9638)
                     /locus_tag="OHN28_001510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9602..9604),aa:Ser,seq:gga)
     gene            complement(9892..10110)
                     /gene="infA"
                     /locus_tag="OHN28_001511"
     CDS             complement(9892..10110)
                     /gene="infA"
                     /locus_tag="OHN28_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCQ2296925.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10395..11099)
                     /gene="aat"
                     /locus_tag="OHN28_001512"
     CDS             complement(10395..11099)
                     /gene="aat"
                     /locus_tag="OHN28_001512"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCQ2296926.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(11141..12862)
                     /gene="cydC"
                     /locus_tag="OHN28_001513"
     CDS             complement(11141..12862)
                     /gene="cydC"
                     /locus_tag="OHN28_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCQ2296927.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12863..14629)
                     /gene="cydD"
                     /locus_tag="OHN28_001514"
     CDS             complement(12863..14629)
                     /gene="cydD"
                     /locus_tag="OHN28_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCQ2296928.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14752..15717)
                     /gene="trxB"
                     /locus_tag="OHN28_001515"
     CDS             complement(14752..15717)
                     /gene="trxB"
                     /locus_tag="OHN28_001515"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCQ2296929.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16261..16755
                     /gene="lrp"
                     /locus_tag="OHN28_001516"
     CDS             16261..16755
                     /gene="lrp"
                     /locus_tag="OHN28_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCQ2296930.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16890..20996
                     /gene="ftsK"
                     /locus_tag="OHN28_001517"
     CDS             16890..20996
                     /gene="ftsK"
                     /locus_tag="OHN28_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCQ2296931.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21155..21766
                     /gene="lolA"
                     /locus_tag="OHN28_001518"
     CDS             21155..21766
                     /gene="lolA"
                     /locus_tag="OHN28_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCQ2296932.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21777..23120
                     /gene="rarA"
                     /locus_tag="OHN28_001519"
                     /gene_synonym="mgsA"
     CDS             21777..23120
                     /gene="rarA"
                     /locus_tag="OHN28_001519"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCQ2296933.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23211..24503
                     /gene="serS"
                     /locus_tag="OHN28_001520"
     CDS             23211..24503
                     /gene="serS"
                     /locus_tag="OHN28_001520"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCQ2296934.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24742..27186
                     /gene="dmsA"
                     /locus_tag="OHN28_001521"
     CDS             24742..27186
                     /gene="dmsA"
                     /locus_tag="OHN28_001521"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCQ2296935.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27197..27814
                     /gene="dmsB"
                     /locus_tag="OHN28_001522"
     CDS             27197..27814
                     /gene="dmsB"
                     /locus_tag="OHN28_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2296936.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27816..28679
                     /gene="dmsC"
                     /locus_tag="OHN28_001523"
     CDS             27816..28679
                     /gene="dmsC"
                     /locus_tag="OHN28_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCQ2296937.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(28715..29341)
                     /locus_tag="OHN28_001524"
     CDS             complement(28715..29341)
                     /locus_tag="OHN28_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2296938.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29655..30803
                     /locus_tag="OHN28_001525"
     CDS             29655..30803
                     /locus_tag="OHN28_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2296939.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(30900..31697)
                     /gene="pflA"
                     /locus_tag="OHN28_001526"
     CDS             complement(30900..31697)
                     /gene="pflA"
                     /locus_tag="OHN28_001526"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCQ2296940.1"
                     /translation="MSNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFIT
                     FFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAIL
                     QAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQN
                     LVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIEL
                     LPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(31729..32724)
                     /locus_tag="OHN28_001527"
     CDS             complement(31729..32724)
                     /locus_tag="OHN28_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2296941.1"
                     /translation="MSIKKLDDGRYEVDVRPQGADGKRIRRKFKTKGEAQAFERHVLV
                     NYHNKEWLEKPADRRTLTELLGRWWIYHGKSHERGDIERGRLTTIIAKFAEMGVSRAD
                     QLTKKTITDYRVVMMNDGLKPASVNRHLAIMSGMFTKLIDAGEYHSHNPFREIKRLRE
                     AVTEMAFLSSEEITRLLSMLDGDELNATLVCLSTGGRWSEVSNLKAEHIINQMVTFMK
                     TKNGKRRTIPVSQDLIKRIKTKNSGRLFNASYYKVRNALREVKPDLPDGQAVHVLRHT
                     FATHFIMNGGNIITLQRILGHSNIQQTMTYAHFAPDFLQDAVTLNPVSGMSIMRP"
     gene            complement(32818..33117)
                     /locus_tag="OHN28_001528"
     CDS             complement(32818..33117)
                     /locus_tag="OHN28_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2296942.1"
                     /translation="MSSEQSEKLKLIRESERLKTKELAELIGINYYTYHGYESGKSKM
                     PMEAGMKLFKHPRFRKYRDWFMFDETDPAAGQIAPALAHIGQDSTTLHHSDQKTG"
     gene            33226..33582
                     /locus_tag="OHN28_001529"
     CDS             33226..33582
                     /locus_tag="OHN28_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296943.1"
                     /translation="MTTNVEISCPTSDEASFENEAKRKSIQISERPSDLLSKEGFALY
                     IGKTPRAVAEMAKAGKLPAFYMTDPLKPRGKAELWINRREWDKYAAQLVDEAPTEWHD
                     WKNRISYSKSRHGRAA"
     gene            33593..33763
                     /locus_tag="OHN28_001530"
     CDS             33593..33763
                     /locus_tag="OHN28_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296944.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPVKK
                     ALTFLKSRRVWK"
     gene            33760..34260
                     /locus_tag="OHN28_001531"
     CDS             33760..34260
                     /locus_tag="OHN28_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HCQ2296945.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            34324..34548
                     /locus_tag="OHN28_001532"
     CDS             34324..34548
                     /locus_tag="OHN28_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HCQ2296946.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            34548..34850
                     /locus_tag="OHN28_001533"
     CDS             34548..34850
                     /locus_tag="OHN28_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HCQ2296947.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDISESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRSW"
     gene            34850..35074
                     /locus_tag="OHN28_001534"
     CDS             34850..35074
                     /locus_tag="OHN28_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCQ2296948.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            35071..35346
                     /locus_tag="OHN28_001535"
     CDS             35071..35346
                     /locus_tag="OHN28_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HCQ2296949.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            35336..37618
                     /locus_tag="OHN28_001536"
     CDS             35336..37618
                     /locus_tag="OHN28_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCQ2296950.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNTPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTATFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNYE
                     WWIHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCHIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADRGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSSPAPTPSEHAAAVLNLVDDGVIEWSDPEVVMALRGALKHGLRTPNRQ
                     QRNGIPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWDLEALARGATVNYDG
                     KKFTYPVADEWSWFYCYSEQ"
BASE COUNT         9334 a         9148 c        10021 g         9152 t
ORIGIN      
        1 agcaagaggg agtataacgc gattattcgc tcatttttca gacatttgcc atgcttaaat
       61 gtgatgccat cacgtattag caggcctttc ccgttatact gccagcgtaa agaataagtc
      121 acatatttct ggaggggata tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat
      181 caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg attggggaga acgcgtgggg
      241 taaatccagc ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca
      301 ttttgagcgc gatgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct
      361 gcatattatt ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgctatcg
      421 gccgctggaa gcgtgctgga cgccatgcac cgatggttat caccgtattt tttatcgtct
      481 ggaaggggag agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga
      541 cggacatccg attgatgttg aggatattaa cgatcaggct cgtcatctgg tgcgtttaat
      601 gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc
      661 aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc
      721 ctcacatccg caaaatctct ctgatggaca gattcgtcag ggactttccg caatggtgca
      781 actgcttgag cattattttt ctgagcaggg agctggacag gcgcgatatc gtttaatgcg
      841 gcggcgagcc agcaatgagc aacgaagctg gcgctatctg gatatcatca accggatgat
      901 tgaccgacct ggagggcgct cgtatcgggt tattttgctc ggcctgtttg ctactttgtt
      961 gcaggcaaaa ggcacattgc gactggataa agacgcccgt ccattgttgc tgatcgaaga
     1021 tccagaaacc cgtttgcacc ccattatgct ttcagttgcc tggcatctgt tgaaccttct
     1081 gccattgcag cgcatcgcta ctaccaactc gggagagttg ctttcgttaa cgccggtaga
     1141 gcatgtttgc cgactggtac gtgagtcctc tcgcgttgcc gcctggcgtc tgggaccgag
     1201 tggcttgagt accgaagata gccgacgcat ctcctttcac attcgtttta accgtccgtc
     1261 atcgctgttt gcccgttgtt ggctgctggt ggaaggggaa acggaaacct gggttatcaa
     1321 tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa gggatcaagg tcattgagtt
     1381 tgcccagtcc gggctaaagc cactggttaa atttgcccgg cgaatgggga ttgaatggca
     1441 tgtactggtc gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt
     1501 gaataatgat cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga
     1561 acattttatg tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga
     1621 aaatataccg atgaatctgc gcaaaattat ctcgaaagcg atccatcgct cttccaaacc
     1681 cgatcttgcc attgaagtgg caatggaggc cggacgtcgt ggtgtggact ccgtaccgac
     1741 gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg cgcggtcggg ccgactaact
     1801 acaaaacatc gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta
     1861 ttcagcccgt tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag
     1921 gcggtaatgg cgttcagcat cacatacgcc gccaaccgat tcccagaaag cgttgtaatc
     1981 ggcatggatc ttgccttctt tatcgcgata acgcaggctg cggtaaatat gcgtttcatt
     2041 gctgacggca ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat
     2101 cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctggtg
     2161 tggaatttcc cattttgcgc cttgcagtcc gccaataaac atcgtccttt tcccctgata
     2221 ttcacacagg gtaaaagtga gctctgccag aggaatacct tcgctgttgc ggaacaggat
     2281 ggtgctgtca ccttctttat ccattgagat catcatggtc agctcaagcg tgaactgctc
     2341 gccgtttttg ccttccagct tcgccagttg cagcccgggg gtattcaaat ataagctgaa
     2401 ttcttccgcc gacatacacc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa
     2461 ttgcttacgg ctaagattcg ccgcaaggta agggcgatgt agacgtacag gcagtcgcgg
     2521 ctggcgcgtt aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac
     2581 gcttaaacgc ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacggcgatg
     2641 ccagaattta cccggccgac actgtcctcg tgccagacta agaaaaagtg acaggctgct
     2701 gagagattca gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcaat
     2761 ggttatacat gacgtcgatt taaccattgc gtatcttaac caaacatcaa tagtgtgatt
     2821 attaacgtaa attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca
     2881 tatggagtat tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt
     2941 gcgatggtga tagtcatcgc cgggctgatt acgttatgga gaattcttaa cgcacccgtg
     3001 ccgacttatc agacgctgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc
     3061 ggaaagctgg acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa
     3121 acgctgtcgg tggcgattgg cgataaagta aaaaaagacc agcttttagg ggttattgat
     3181 cctgaacagg ctgaaaacca gatcaaggag gtggaagcaa cgctgatgga gctacgcgcg
     3241 cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa
     3301 cgtcttgcac aaacgcaagc ggtttcacag caggatctcg ataacgccgc gacggagatg
     3361 gctgtgaaac aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcctct
     3421 ctcgatacgg ctaaaaccaa tctcgattac acccgcatcg ttgccccgat ggccggggaa
     3481 gtcacgcaaa tcaccactct gcaaggccag acggtgattg ccgcacaaca agcaccgaac
     3541 attctgacgc tggcagatat gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat
     3601 gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga tccactgacg
     3661 cgctacgagg ggcaaatcaa ggatgtgcta ccgacgccgg aaaagattaa cgacgctatt
     3721 ttctattacg cccgttttga agtacccaac cccaatggtt tactgcggct ggatatgacc
     3781 gcgcaagtgc atattcagct caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg
     3841 ttaggcgatc cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca
     3901 cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg
     3961 ctggaagcgg gcgatgaagt ggtgattggt gaggccaaac caggagctgc gcaatgacgc
     4021 ctttgctcga attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg
     4081 tgctgaaggg catcagcctc gatatttatg ccggtgagat ggtagcgatt gtcggtgctt
     4141 cgggttctgg taaatcgacc ctgatgaata ttctcggctg cctggataag gccaccagcg
     4201 gcacctatcg cgtcgccggt caggatgttg ccacgctgga cgccgatgcg ctggcgcaac
     4261 tgcgccgcga gcatttcggc tttattttcc agcgttacca tttgctttcg catttaaccg
     4321 ccgagcagaa cgttgaagta cccgccgtct atgccggtct tgagtggaaa cagcggctgc
     4381 ttcgcgccca ggagttactg caacgactgg ggctggaaga ccgtacagag tattacccgg
     4441 cacagctttc gggtggccag caacagcgcg tcagtatcgc gcgggcattg atgaacggcg
     4501 gtcaggtaat tcttgccgat gaaccgaccg gcgcactgga cagccattct ggcgaagagg
     4561 tgatggcgat cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc gtcacacacg
     4621 atccgcaggt tgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc
     4681 gcaatcctcc cgccattgaa aaagtgaatg ctactggcgg aacggaacct gttgtcaaca
     4741 cggcgtctgg ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc
     4801 gggcgctggc agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta
     4861 ttgcgtcggt ggtttccatt gtcgtggtgg gcgacgccgc caaacaaatg gtgctggcgg
     4921 atattcgttc tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg
     4981 acgatccgca atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac
     5041 cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg
     5101 ttgatgtggc tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga
     5161 ctttcagtga agggaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg
     5221 ttctcgacag taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg
     5281 tgattctggt tggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga
     5341 tgtttggtag cagtaaagtg ctgcgtgtct ggctgccata cagcacgatg tccgggcgag
     5401 ttatgggtca gtcgtggctg aactccataa ctgtcagggt gaaagaaggc tttgacagcg
     5461 ctgaggcgga acagcaactc acgcgtttac tttcactgcg ccacggtaag aaggatttct
     5521 ttacctggaa catggatggc gtcttgaaaa ccgttgaaaa gaccacacgt actttacaac
     5581 tgtttctgac gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata
     5641 ttatgctggt gtcagtgacc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg
     5701 cgcgagcaag cgatgttttg caacagtttc tgatcgaagc cgtactggtt tgcctggttg
     5761 gtggcgcttt gggaataaca ctgtcactgt taattgcttt caccttgcag cttttcttac
     5821 ccggctggga gattggtttt tcaccgttag cgctgctgct ggcgtttctc tgctcgacgg
     5881 tcaccgggat tttatttggc tggttacccg cacgaaatgc ggcacgactg gatccagtag
     5941 atgctctggc acgggagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt
     6001 gcctttagga tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg
     6061 ggcacaataa cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat
     6121 tgtccagctt ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata
     6181 tcttcgccgc cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta
     6241 acagtaccct tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata
     6301 aaccggggtc agagaggggg ctgttcaaaa cctcgccaac tctagaaata caatttagag
     6361 aattagggcg agccgtcaag catttgacag gggccagggg gcaggtatga atcaaaaatt
     6421 tgaagcagtt aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg
     6481 atagttatct atcatgtgga gtagattggt caggcaaata agctcttgtc agcggcaggg
     6541 cgttctgccg ataaccgtaa ccgaagatga taactgacaa tgggtaaaac gaacgactgg
     6601 ctggactttg atcaactggc ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg
     6661 tataaagtga tattagtcaa tgatgattat actccgatgg agtttgttat tgacgtgtta
     6721 caaaaattct tttcttatga tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac
     6781 caggggaagg ccatttgcgg agtctttacc gctgaggttg cagaaaccaa agtggcgatg
     6841 gtgaacaagt acgcgaggga gaatgagcat ccattgctgt gtacgctaga aaaagcctga
     6901 atgcaggcat aaaaattggg ggaggtgcct atgctcaatc aagaactgga actcagttta
     6961 aatatggctt tcgccagagc gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg
     7021 ttactggcgc tgctcagtaa cccatctgcc cgggaggcgc tggaagcgtg ttctgtagat
     7081 ttggttgcgc tccgtcagga actggaagcc tttattgaac aaaccacacc cgttctgcct
     7141 gccagtgaag aggagcgcga cacacagccg acgttgagtt ttcagcgtgt actgcaacgt
     7201 gcggtcttcc atgtccagtc ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc
     7261 gctatcttta gcgaacagga gtcgcaggcg gcatatctgt tgcgcaaaca tgaagtcagc
     7321 cgtctcgatg tggtgaactt tatctctcat ggcacgcgta aagacgagcc gacacagtct
     7381 tctgatcctg gcagccagcc aaacagcgaa gaacaagctg gtggggagga acgtatggag
     7441 aatttcacga cgaacctgaa tcagcttgcg cgagtgggcg gaatcgaccc actaattggt
     7501 cgtgagaagg agctggagcg tgctattcag gttctctgcc gtcgccgtaa aaacaacccg
     7561 ctgctggtgg gggagtctgg tgtcggtaaa accgcgattg cggaggggct tgcatggcga
     7621 attgttcagg gcgatgtgcc ggaagtgatg gctgactgta cgatttactc tctcgatatc
     7681 ggttccctgt tagcgggcac aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg
     7741 ctcaagcagc tggagcagga cactaacagc atcctgttta ttgatgagat ccacaccatt
     7801 atcggtgcgg gtgcagcgtc tggtggccag gtcgatgcgg ctaacctgat taaaccgttg
     7861 ctctccagcg gtaaaatccg cgtaattggt tcgacaacct atcaggagtt cagcaacatt
     7921 ttcgagaaag accgtgctct ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtca
     7981 atcgaagaaa ctgttcaaat catcaatggc ctgaaaccga agtatgaagc gcaccacgac
     8041 gtgcgttata ccgcaaaagc ggtgcgtgcg gcggtagagc tggcggtgaa atacattaac
     8101 gatcgtcatc tgccggataa agcgattgat gttatcgacg aagcgggcgc tcgcgcacgt
     8161 ctgatgccgg taagcaaacg caagaaaacc gttaatgtgg cggatattga atccgtggtg
     8221 gcccgtattg cacgcattcc agagaagagt gtttctcaga gtgaccgcga taccctgaaa
     8281 aacctcggcg atcgcctgaa aatgctggtc ttcggtcagg ataaagccat tgaggcgctg
     8341 actgaagcca ttaagatggc gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg
     8401 ttcctgtttg ccggccctac tggggtcggg aaaacagagg tgacggtaca gctttcgaaa
     8461 gcgttgggca ttgagcttct gcgctttgat atgtccgagt atatggaacg ccataccgtc
     8521 agccgtctga ttggtgcgcc tccgggatac gttggttttg atcagggcgg tttgctgact
     8581 gatgcggtca tcaagcatcc acatgcggtg ctgctgctgg acgaaatcga gaaagcgcac
     8641 ccggacgtgt tcaatattct gttgcaggtg atggataacg gtacgctgac cgataacaac
     8701 ggacgcaaag cggacttccg taacgtggtg ctggtgatga ccactaatgc cggggtacgg
     8761 gaaactgagc gcaaatccat tggtcttatc caccaggata acagcaccga tgcgatggaa
     8821 gagatcaaga agatctttac accggaattc cgtaaccgtc tcgacaacat tatctggttc
     8881 gatcatctgt caaccgacgt gatccatcag gtggtggata aattcatcgt cgagttgcag
     8941 gttcagttgg atcagaaagg tgtttctctg gaagtgagcc aggaagcgcg taactggctg
     9001 gccgagaaag gttacgaccg ggcaatgggc gcacgtccga tggcgcgtgt catccaggac
     9061 aacctgaaaa aaccgctcgc caacgaactg ctgtttggtt cgctggtgga cggcggtcag
     9121 gtcaccgtcg cgctggataa agagaaaaat gagctgactt acggattcca gagtgcacaa
     9181 aagcacaagg cggaagcagc gcattaatct gattgtctgg taggttggtg aagtccgtaa
     9241 tctcgtcagg ggttacggac tttttattta tggggggagg aggttcagac cctttttttg
     9301 atgatgatgg taagttattg ataattagtg ctgcgggtag gtaaggataa aaaagggtgg
     9361 cagcaggaga ttgagatggt tttgctttat taacaacggg ctaaacgtgt agtatttgag
     9421 ttcactgccg tacaggcagc ttagaaattg ccgcggatcc tgtctgccaa taatgacaag
     9481 ttcactgccg tacaggcaga taaaatgcga aaaaaaagcc cgtactttcg tacgagctct
     9541 tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
     9601 ttccaggcgt gctccttcag ccactcggac acctcaccaa attgtttttt tgcctgacct
     9661 catggggggc aacggggcgc tactataggg agttggaata aaacggtcaa gaagaatttt
     9721 tatgataatt attgtttgct catactgtaa acaagttgtg cagtatatct acatcgagac
     9781 aagttacgga cttatacttc taaagtactc catacatatc acaaaataaa aaggccggct
     9841 aaaccgacct tttactcatt ctttctcttc gcccatcagg cggtaaaaca atcagcgact
     9901 acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag tcactttgtc
     9961 gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg cagtaaccac
    10021 gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa gaacggtacc
    10081 ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac taccgtaatt
    10141 tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc gctaaatcgc
    10201 aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc aaaagcgcaa
    10261 cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg
    10321 gcagcggggt aatgagcgta accaactctg cgaccgcaat tataacactc tggggagaaa
    10381 tgtgccgaaa acattcattc ttgtggtgaa aacaagcatc gaggtaccca gaaattattc
    10441 gacaatcgtc cgaggcgcat ttgattgaga taattaaggt aatcccggcg gggaatttcg
    10501 caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag tttaccgcca
    10561 tgacggataa attcatcaca gaagaccaga agcgccgttt tggacgcatt ttccatccgg
    10621 ctgaacatgg actcgccaca aaatagtgtt ccctgggcca cgccgtacat accgccgaca
    10681 agctcatctt cacgccagac ttcaatggag tgggcatgac cgagttcgtg aaggcgatgg
    10741 taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct ggcacagcct
    10801 tcgatgacct gaccaaaagc gtaattcatc gtgacacgat agggtgagcg tttatgaaat
    10861 cgcttcatac tacggctgat atgcagtgat tctggccata acaccgcgcg gggatcgggc
    10921 gaccaccaga ggatggggtc gccaggagaa aaccacggga aaataccacg ctgataagcc
    10981 attaataggc gcgcagggct aagatcgccc cccagtgcca gcaggccgtt aggctcacgt
    11041 aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac caggcgcatg
    11101 accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc tgcttaaact
    11161 ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt accttgctca ataatttgcc
    11221 cgttgtccat cactattatt tgttggaaac gagagagccc gcgaagtcga tgggtgacca
    11281 ttaacaccgt tttctcgcac atcatttctg caagcaattc aaggatctga ctttcggttg
    11341 tggcatctaa gccttctgta ggttcatcca gcaataccag cggcgcatca tgtaacagcg
    11401 cacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg ccttcaccta
    11461 accaactgtt gagacctgca tcctcgagca gcttctccag accaacgcga cgcaagatct
    11521 ccgccagagc ctcatcacta ctaccaggcg aggcgagtaa cagattatca cgcagcgtgg
    11581 cgctaaacag atgcactcgc tggggaacaa cgctgatggt ctgtcgtaga gccgcttcat
    11641 tcaggctggc tatgggacta tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc
    11701 gggtcagcag ttgtaacagt gttgacttgc cgcatccggt tcgcccgaga atcgcaatat
    11761 gttccccggc gtttacctga agataaatcc ctttaagtgc ctgttgggat tgctccggat
    11821 aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat
    11881 cagggaaggt gactttcggt ttttgatccg ttaaatcagt gatccgtacg gcagaggcaa
    11941 tgacttgccc cagatgctga aatgcacccg ttactggtgc cagcgcttca aacgcggcta
    12001 acgcgcagaa gacaaacagg gcaattaacg cgccgggttg agcattgccg ccaacgccgc
    12061 cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc attatcgctt
    12121 gcgacaatgc ggttagttca gattgacggc gttgcgcttc cagccattga atttctgtat
    12181 tctctaattg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca gcttgccctt
    12241 gcagccaggc cgtcagttgt tggcgatact gcccgcgaag atgagtcaga ttttgcccgg
    12301 tgctttttcc cgcacgataa aacagtggtg gcatcaggaa aagcgtcagt aacataatgc
    12361 cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc accaccataa
    12421 tcaccacaaa agcgcctacc agcggcgaga taacgcgcag gtaaagatga tcgagcgtat
    12481 caacatccgc caccacgcgg ttgagtaatt cgccctgacg atagcgcgcc agtcctgcag
    12541 gggagagggg cagtaatttg ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag
    12601 tcgcatcgtg acttaccagg cgttcaaaat agcgcccggc agtacgggtg attgctgcgc
    12661 cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca acccccgcaa
    12721 ccgctgaggc ggagaggaac cagcccgaaa gtgtcaacag accgatactg gcgagcagcg
    12781 tgacaattgc cagcacgata ccaagactta acatccattt atgacgttta tacagtgcca
    12841 gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt aatgtggcga
    12901 atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc tgaccatcct
    12961 gcattaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg accattaacg
    13021 ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca ctgtgagcat
    13081 caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt aacgcacggg
    13141 ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg
    13201 tatcaacgcc ttgtggcagg agcggtagaa actcactgac ccaggcgtta tccagcgctg
    13261 tttgtaactc ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg
    13321 ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg tttacgccat gattccggtg
    13381 ataaatcgcg taattctatc ccgttgattc gtagcgatcc ctgatatgag agaaaaccag
    13441 aaagcgcgtt caacagtgaa cttttacctg aaccgctgcg accaaccaac actgctcgtt
    13501 ggcctgctgg caaagtaaaa ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga
    13561 taaacagatc ctcggcttca atggtcaccg gatcggtcga tgctaattcc gcttcaccgc
    13621 gctgcggatg ggctagcggg gtttccataa acgttttcaa actgtcagct gcaccaacag
    13681 cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa aactctggcg
    13741 caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat
    13801 ccagctcgcc gaggtaggaa aaaccaaagt agaccgccac cagagcaatc gacagcgagg
    13861 taaaaaattc gagaatgccg gaggataaaa acgccagccg tagcacttcc attgtccgtt
    13921 ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca cgaccaaaaa
    13981 tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag cgagcaagag
    14041 cgagaaagtt acgtcggtta gcatcggcag cccccattcc aaccagcgcc ataaataacg
    14101 gaattagcgg tgcagtgccc agcagaatga gcgccgcggc ccagttagag gggaagatag
    14161 ccaccacaat cagcagcggc accgacactg ccagcgccat ttgcggcagg tagcgtgcat
    14221 agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc cccgcaggtt
    14281 taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctga cggcggatgg
    14341 caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag accacccatg
    14401 cgcgcagtac aaaggtcaga aacagtaacg taaagggaag cagcagggct tcacggggaa
    14461 tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg gcaatgatca
    14521 atatgccgct cacaaagccc agcagacgag aaatattcag ccaacgttgg gagatgacgc
    14581 tttgctgttt taaccagcgg gttaattctt tttgacggga tttattcatt gcacgcttag
    14641 caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa ataaaggcga
    14701 cccatagtcg catggtgtcg ccttctttac ttttgttact gatttgtaaa attattttgc
    14761 gtcagctaaa ccgtcgaggt agcgttccgc atcaagagct gccatgcagc ctgtaccagc
    14821 agatgtaatg gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa agacgccagg
    14881 gatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt agccgttttc
    14941 cagttccagc tgcccttcga aaatggcagt attcgggctg tgaccgatag caacaaacag
    15001 accggcaacg tcgagtgact cgatattatc ggtgttttgc gtatcgcgca gacgaacgcc
    15061 agtgacgccc atctgatcgc cggtcacttc ttccagcgta cggttggtgt gcagaataat
    15121 gttgccgttc tccactttat ccatcagacg cttaatgagg attttttccg cacggaaacc
    15181 atcacggcgg tgaatcagat gaacttccga agcgatgtta gacagataca gcgcctcttc
    15241 aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga agaaaccgtc
    15301 gcaggttgca caagcagaaa ctccacggcc tttaaaggct tcttcagagg gcaggccgag
    15361 atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt cgccgctatc
    15421 gccagtcaga cggaacggac gattctgcaa atcaactttg ttgatgtgat caaagatgat
    15481 ttcagtttca aatttggtgg catgttcgtg catgcgctcc attaataacg gaccggtcag
    15541 atcgtttgga tcgccaggcc agttttccac ttccgtggtg gtggtcagtt ggccgccttt
    15601 ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcgtaga cagcagcggt
    15661 gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg tgcccatgag
    15721 atccccatag ttgttggcag acaatggcag gattgtaggg aatttacaga cgtaaaaaaa
    15781 gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc aggtcaaatt
    15841 ctcacgtcat tatcacctct gctacttaaa tttcccgctt tataagccga ttaaatgatg
    15901 aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat gttttgcttt
    15961 gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt attcttatat
    16021 gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt
    16081 ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc tcaccgtaga
    16141 cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg gtagcgactc
    16201 tgaacagtga tgtttcaggg tcagacagga gtagggaagg aatacagaga gacaataata
    16261 atggtagata gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg taacattctt
    16321 aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg tgtgggactt
    16381 tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc
    16441 tatacggcgc tgctgaaccc ccattatctg gatgcatcac ttctggtatt cgttgagatt
    16501 actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt acaaaaactt
    16561 gaagaaattc aggagtgtca tttagtatcc ggtgatttcg actacctgtt gaaaacacgc
    16621 gtgccggata tgtctgctta ccgtaagtta ctgggggaaa ccctgctgcg tctgcctggc
    16681 gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa tcgtctggtt
    16741 attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta cactcctgtt
    16801 aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta gcatcgggca
    16861 ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa gacaaagaag
    16921 tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg atccttattg
    16981 tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg gaccccagct
    17041 ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc ggtgcgtggc
    17101 tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc gtcattattg
    17161 tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt gattattttg
    17221 ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg
    17281 caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc agcttactaa
    17341 gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg ctctgcgttt
    17401 gggcagcggg cctgacgctg ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg
    17461 gcggctggat tttaaatatt cttaccttcg ccagtaatcg tacccgtcgc gatgatacct
    17521 gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat cacggcaaac
    17581 agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt aaacggttgg
    17641 cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc tccggtaagc
    17701 ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc gaccctgacg
    17761 acgtcctctt ttcgggcaat cgtgcaacgc agccagaata tgacgagtac gatccattat
    17821 taaacggtgc gccaattacc gaacctgttg ctgtagcagc tgctgctacc acggcgacac
    17881 aaagctgggc tgcaccggtt gaacctgtga ctcagacgcc gccagttgcc tctgttgatg
    17941 ttccacctgc gcaatctaca gtagcctggc agcctgtacc ggggccgcaa acgggagaac
    18001 cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg cagcctgcag
    18061 tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat tatgcacctg
    18121 cagctgaaca acctgcgcaa caaccttact acgctcctgc ggcggaacaa cctgttcagc
    18181 aaccgtatta cgcaactgcg gcggaacagc ctgcgcaaca gccgtattac gcccctgcgc
    18241 cagaacaagc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc
    18301 cacagtctac ataccagact gagcaaactt atcagcagcc agccgctcag gaaccgttgt
    18361 accaacagcc gcaacctgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag
    18421 agacaaaacc cacgcgtccg ccgctttact actttgaaga agtggaagag aagcgagccc
    18481 gtgaacgcga acaacttgcg gcctggtatc aaccgattcc agaacctgtt aaagaaccag
    18541 aaccgatcaa atcttcgcta aaagcacctt ctgttgcagc agtacctcca gtggaagccg
    18601 ctgccgccgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg
    18661 ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg
    18721 tcaaagaggg gattggcccg cagttgccac gaccgaaacg catccgcgtg ccaactcgtc
    18781 gtgaactggc gtcttacggt attaagctgc cctcacagcg tgcggcagaa gaaaaagccc
    18841 gtgaagccca gcgtaatcag tacgattctg gcgatcagta caacgatgat gaaatcgatg
    18901 cgatgcagca ggatgaactg gcacgtcagt tcgcccagac gcagcagcaa cgctatggcg
    18961 aacagtatca acatgatgtg cccgtgaaca cagaagatgc agatgctgcg gcagaggcag
    19021 aactggctcg tcagtttgct caaactcaac aacaacgtta ttccggcgaa caaccggctg
    19081 gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg
    19141 atggtccaca cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac
    19201 cggtagcacc gcagcagcaa tatcagcagc cgcaacaacc ggttgcaccg cagccgcagt
    19261 atcagcagcc gcaacaaccg gttgcaccgc agcagcaata tcagcagcca caacaaccgg
    19321 ttgcacagca gccgcagtat cagcagccac aacagccggt tacacagcag ccgcagtatc
    19381 agcagccgca gcagccggtt gtaccacagc cgcaatatca gcagccgcaa cagccagttg
    19441 cgccacagcc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc
    19501 cgttgcataa accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg
    19561 aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgcctggta gaagcgcgtc
    19621 tggctgattt ccgtattaaa gccgatgttg tcaattactc tccggggcca gttatcactc
    19681 gctttgaatt gaacctggca ccgggcgtaa aagcggcgcg catttctaac ttgtcacgtg
    19741 accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac
    19801 cctatgtagg tctggagtta ccgaataaaa aacgacaaac cgtttatctg cgcgaggttt
    19861 tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata
    19921 tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga
    19981 ctaccggttc cggtaagtct gtcggggtga acgcgatgat cctgagcatg ctttataaag
    20041 cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt
    20101 atgaaggcat tccgcacctg ttaacggaag ttgttactga tatgaaagat gccgccaacg
    20161 cgctgcgctg gtgtgttaac gagatggagc gtcgttataa actgatgtct gcgctgggtg
    20221 tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga
    20281 ttccagaccc gtactggaag ccgggtgata gtatggatgc gcagcatccg gtgctgaaaa
    20341 aagaaccgta catagtggtg ttggtggacg aatttgccga cctgatgatg acggtaggta
    20401 aaaaagtgga agagctgata gcacgtctgg cgcaaaaagc tcgtgccgcg ggtatccacc
    20461 tcgtactggc aacgcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata
    20521 ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc
    20581 aggctggtgc ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca
    20641 ctttgccggt acgtgtccat ggtgcttttg ttcgcgatca agaagttcat gccgtggtgc
    20701 aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa
    20761 gcgaaggtgg cgcgggcggt ttcgatggcg ctgaagagct ggatccgttg ttcgatcagg
    20821 cggtgcagtt tgtcactgaa aaacgcaaag catcaatttc tggcgtacag cgtcagttcc
    20881 gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca
    20941 gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa
    21001 tgcatcgtag gccggataag gcgcggtagc gtcgcatccg gcaatctggt aactgaaaat
    21061 tcagtatttt cttctttcct caagctgatt attagcctgg aatagagagt agagggagct
    21121 cccggtcggg agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca tcacctgtgc
    21181 attactctca agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag
    21241 ccgcctggat aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag
    21301 cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa
    21361 ctggcatatg actcaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt
    21421 ctataacccg ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac
    21481 gccgtttatg ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca
    21541 gaatggcgat gactttgttc tgacgccgaa agccagcaat ggcaatctga agcagttcac
    21601 cattaacgtg ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca
    21661 gcgcagcagt tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac
    21721 cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga
    21781 gcaatctgtc gctcgatttt tcggataata cttttcaacc cctggccgcg cgtatgcggc
    21841 cagaaaattt agcacagtat atcggccagc aacatttgct ggctgtgggg aagccgttgc
    21901 cgcgcgctat cgaagccggg catctgcatt ctatgatcct ctgggggccg ccgggtaccg
    21961 gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt
    22021 ctgccgtcac ctctggcgtg aaagagattc gcgaggcgat cgaacgcgct cggcaaaacc
    22081 gcaatgcagg tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc
    22141 agcaggatgc atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca
    22201 ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtctatctgt
    22261 tgaaatccct gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa
    22321 cccgtggcta tggtggtcag gatattgttc tgccagatga aacacgacgc gccattgctg
    22381 aactggtgaa tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg
    22441 ccgaagtcga tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg
    22501 gtgaacgtag cgcccgcttt gataacaaag gcgatcgctt ttacgatctg atttccgcat
    22561 tgcataagtc ggtacgtggt agcgcacccg atgcggcgct ctactggtat gcgcgaatta
    22621 ttaccgccgg tggcgatccg ttatatgttg cgcgtcgttg cctggcaatt gcttccgaag
    22681 atgtcggcaa tgccgaccca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta
    22741 ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct
    22801 gcgcgccaaa aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg
    22861 aacgtccgga ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg
    22921 aaatgggcta cgggcaggaa tatcgttacg ctcatgatga agcaaacgct tatgctgccg
    22981 gtgaggttta cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg
    23041 gccttgaagg caagattggc gaaaagctcg cctggctggc tgaacaggat caaaatagcc
    23101 ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt tgttaatgta tccctgtggt
    23161 cgcaggctgt ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc
    23221 ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta
    23281 agctggatgt agataagctg ggcgctcttg aggagcgtcg taaagtattg caggtcaaaa
    23341 cggaaaacct gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc
    23401 gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg
    23461 cagcaaaagc cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctaaccatcc
    23521 ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca
    23581 gtcgctgggg caccccgcgt gagtttgact ttgaagttcg tgaccatgtg acgctgggtg
    23641 aaatgcactc tggcctcgac tttgcagccg cagttaagtt gactggttcc cgctttgtgg
    23701 taatgaaagg gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc
    23761 ataccgaaca gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc
    23821 tgtacggtac gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg
    23881 aagaagaagc agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacca
    23941 acctggtacg cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca
    24001 ccccatgttt ccgttctgaa gctggttcat atggtcgtga cactcgtggt ctgatccgta
    24061 tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac tcaatggcgg
    24121 cgctggaaga gatgaccggt catgcggaaa aagtcctgca gctgctgggc ctgccgtacc
    24181 gtaaaatcat cctttgtacc ggcgacatgg gctttggcgc ttgcaaaact tatgacctgg
    24241 aagtatggat cccggcacag aacacctacc gcgagatctc ttcctgctcc aacgtttggg
    24301 atttccaggc acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc
    24361 tggttcatac cctgaacggt tctggtcttg ctgttggtcg tacgcttgtt gcagtaatgg
    24421 aaaactatca gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga
    24481 atggactgga atatattggc taatacccaa tttttctgaa tctaaaaagc gcctgcgggc
    24541 gctttttttg tctccctttg ataccgaaca ataattactc ctcacttaca agtaatacta
    24601 ctttcgagtg aaaatccacc tatctctttg attttcaaat tattcgatgt atacaagcct
    24661 atatagcgat ctgctataga aataattaca caatacggtt tgttactgga atcaatcgtg
    24721 agcaagcttg agtgagccat tatgaaaacg aaaatccctg atgcggtatt ggctgctgag
    24781 gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat ggccagcagc
    24841 gcattaacat taccttttag tcggattgcg cacgctgtcg atagcgccat tccaacaaaa
    24901 tcagacgaaa aggttatctg gagcgcctgt acagttaact gtggtagtcg ctgcccgcta
    24961 cgtatgcacg tcgtggacgg tgaaatcaaa tatgttgaaa cggataatac tggcgatgac
    25021 aactatgacg gcctgcacca ggtgcgcgcc tgtctgcgtg gtcgttccat gcgtcgccgc
    25081 gtttacaatc ccgaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc
    25141 aaattcgagc gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc
    25201 ctgatcaaag agtacggcaa cgaatccatc tatctgaact atggcaccgg tacgctgggt
    25261 ggcaccatga cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc
    25321 tgcggcggtt atctgaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg
    25381 aactatacct acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa
    25441 ctggtagtgc tgtttggtaa taaccctggc gaaacgcgaa tgagtggcgg tggggtgact
    25501 tactatcttg aacaggcgcg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc
    25561 tataccgaca ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc gggaacagat
    25621 gccgcactgg ttaacggtct ggcgtatgtc atgatcaccg aaaacctggt ggatcaggca
    25681 ttcctcgata aatattgcgt tggctacgac gagaaaacac tgccagccag tgcgccgaaa
    25741 aatggtcact ataaagctta tattctgggt gaagggccag atggcgtggc taaaacgccg
    25801 gaatgggcct cgcaaatcac tggtgttccg gcagacaaaa tcatcaaatt ggctcgtgaa
    25861 atcggtagta ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac
    25921 ggtgaaatcg caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt
    25981 aacggaggca acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg
    26041 accctcgaaa acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa
    26101 cgtggcccgg aaatgacagc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg
    26161 ccgatcaaaa tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc
    26221 aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg agctgattgt ggttatcgac
    26281 tgccacatga cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttca
    26341 gaacagatgg actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac
    26401 gatcaggtga ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg
    26461 gcaaaacgtc ttggcgttga gcaacagttt actgagggcc gtacccagga agagtggatg
    26521 cggcatctgt atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc
    26581 cgtaagcagg ggatttttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagct
    26641 ttccgtgaag atccgcaggc aaatccactg accacgccat cggggaaaat tgagatttat
    26701 tcgcaggcgc tggctgacat tgccgctacc tgggaattgc cagaaggtga tgtgatcgat
    26761 ccactgccga tctatacgcc gggttttgaa agttatcagg atccgctgaa caaacagtat
    26821 ccgctgcagc ttacgggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt
    26881 gatgtgctga aagcagcttg ccgtcaggaa atgtggatca acccgcttga tgcgcaaaaa
    26941 cgcggtatca acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt
    27001 gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg cactgggtga aggtgcctgg
    27061 tatgacccgg atacaaaacg tgtcgataag ggcggttgta ttaacgtact gaccactcaa
    27121 cgtccgtctc ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag
    27181 gtgtaaggag taaccgatga caacccagta tggatttttt attgattcca gccgttgcac
    27241 cggttgcaaa acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag
    27301 cttccgccgc atttatgaat atgctggtgg cgactggcag gaagataacg gtgtctggca
    27361 ccagaacgtg tttgcctact atctgtcgat ttcatgtaac cactgcgaag atccggcttg
    27421 tactaaagtc tgcccgagcg gtgcgatgca taaacgtgaa gatggttttg ttgtggtcga
    27481 tgaagatgta tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca
    27541 atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga
    27601 gggaaaaaaa ccgatctgcg tagagtcctg cccgctacgg gcgctggatt tcggccctat
    27661 cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca
    27721 ctttaccaaa ccgaatattg tgatcaaacc caatgccaac agccgcccaa ccggggatac
    27781 cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc
    27841 cgctgatgat cttcacggtc ttcgggcaat gtgtcgcggg tggttttatc gttctggctt
    27901 tggcgctgct caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt
    27961 tgggtttatg ggtgctgatg ggcattggct ttatctcctc tatgcttcat cttggttcac
    28021 cgatgcgcgc ttttaactcg ctcaatcggg taggggcttc agcactcagt aacgaaatcg
    28081 ccagcggttc gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa
    28141 agctttcacc ggcattgcgt acgctttggc tggtggtgac gatggttctt ggcgtagtct
    28201 ttgtctggat gatggtgcgt gtgtataaca gcatcgatac agtacccacc tggtacagca
    28261 tctggacacc gatgggcttc ttcctgacga tgtttatggg cggcccgtta ctgggttacc
    28321 tgttgctgag tctggctggc gtcgatggct gggcgatgcg tctgctgcca gccatttctg
    28381 ttctggcact ggtagtgagt ggcgtgatgt cagtgatgca gggcgcagag ctggcgacta
    28441 ttcatagctc tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct
    28501 ggcggatcgt gcttttggcc gttgccctgt gcgtgtggat tgcaccacag ctaaaaggtt
    28561 atcagcctgc ggttccgcta ctttctgtat cattcattct gcttctggca ggggaattaa
    28621 tcggtcgtgg cgtattctat ggtttgcata tgaccgtggg gatggctgtc gcaaattaat
    28681 cagtaacaac cggggtttcg gccccggttc gcttttattt ctgcttcgtt aacgtgtcat
    28741 aactggtcat caagttacga taatcaggaa tgtggttaga gaacaacgtc gccagtcctt
    28801 caatatcatt acgccagtcg cgatgcagct cgcacgccac gccgaaccat gtcatcaatt
    28861 gcgcaccagc ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca ttaaaggtcc
    28921 ctgacgcgtc agtcacgaca aagacgtcaa acccttcttc aatggctgaa agcgccggga
    28981 atgccacgca aacttcggtt accacaccgg caataattaa ctgtttttta cctgtcgctt
    29041 tgacagcttt tacaaaatct tcgttatccc aggcgttaat atttcccggg cgagcaatgt
    29101 aaggtgcatc ggggaattgt gctttcagtt ctggaactaa tgggccgtta ggaccggttt
    29161 caaaactggt ggtaagaata gttggtaagt tgaaatactt agccaggtca cccagcgcca
    29221 gcacgttatt tttaaactta tcgggttcga tatcccgtac aagggaaagt aaaccagcct
    29281 ggtgatcaac aagcaaaacg gcagcatcat ttttatcaag acgaacatac ggtttggtca
    29341 tcctcttctc ctttcgagag tcagtggtac gtctaaatcg gtcagacgcg atgcattgct
    29401 ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga
    29461 agcgtatgaa atttgtgtta gatcaataaa aacaatcaga tgagattaat cgtctcttca
    29521 gcattagcgt tatgaatgag taatataaat cgataatacc gccgctgcga tatgcgcgta
    29581 ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct
    29641 cacggtttta attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc
    29701 ttttgttgac tctggcgatc gcggtgttaa atacactcgt gccgctttgg ctcgcccagg
    29761 aacacatgtc tacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaaccttg
    29821 tcggtacatt gctgacaggg tatgtcatta aacgcattgg ctttaaccgc agctattatc
    29881 tggcctcctt catttttgcc gctggctgtg ccggccttgg cctgatgatt ggattctgga
    29941 gttggttggc gtggcgtttt gtcgctggca tcggctgtgc catgatttgg gtggttgttg
    30001 agagtgcgct gatgtgcagt gggacgtcgc gtaaccgcgg gcggttgctt gctgcgtata
    30061 tgatggttta ttacgtcgga acgtttttag gccagttact ggtcagcaaa gtctcaaccg
    30121 agttgatgag tgtattgccg tgggttacag gtttgacgtt ggcaggaatc ttaccgctgt
    30181 tgtttacgcg tgtcctgaat cagcagactg aaaaccatga ttcgacgtca attacgtcaa
    30241 tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg cattatctca ggaatcgtgc
    30301 tgggatctct atatggcctg atgccgctgt acctcaatca caaaggggtg agcaatgcca
    30361 gcattggttt ctggatggcg gtactggtca gtgcgggtat cctcggacaa tggccgattg
    30421 gacgtctggc ggataagttt ggtcgactgc tggtgttgcg tgttcaggtc tttgtcgtca
    30481 ttctcggcag tattgcgatg cttagccagg cggcgatggc cccagcgtta ttcatcctcg
    30541 gtgccgctgg ctttacgcta tatccggtgg cgatggcctg ggcttgtgag aaagttgaac
    30601 atcatcaact ggtggcgatg aaccaggcct tactgttgag ctataccgtg ggaagcctgc
    30661 ttggcccgtc atttaccgct atgctaatgc agaatttctc cgataattta ttgtttatca
    30721 tgatcgccag cgtatcgttt atctatttgc tgatgctgct tcgcaacgcc ggccatacgc
    30781 cgaaacccgt ggctcacgtg taaatgaatt caagcagagt gtgaacttac tgtttcacac
    30841 tctgcttttc tgtgcacaaa aaagcggcaa ctcaataaag ttgccgcttt acggggaaat
    30901 tagaacatga ccttatgacc gtactgctca agaatgcctt tcacgcgttc catggtctct
    30961 ttcttcggtg gtttaacacc atcgagtttg tattcttcac ccattgccac ccatttgtgt
    31021 ttgcccagtt cgtggtaggg gaggagctcg attttctcaa cgttgcccat atcacgggta
    31081 aattcaccaa ggcgatgcgc tgaatcgtca tcgtcagacc agcctgggac aacaacatag
    31141 cggatccaca ccttcacatt tttgttcgcc agatatttag cgaactccag cgtgcggtgg
    31201 ttggaaactc caaccagatt ttggtggatc tcgtcgttca tctgtttgag atcgagcatt
    31261 accaggtcgg ttacctccag cagttcatca atcaccggat cgtaacgacg aacaaaaccg
    31321 ttggtgtcca gacaggtatg aatgccttct tttttgcagg cgcggaacca gtcacgaaca
    31381 aactcagctt gtaggattgc ctcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc
    31441 ataaagtggc gataggtcac cacttccttc atcaaatctt caacggtaac ttctttaccg
    31501 ccatgcgtat cccaggtgtc gcggttatga caatacaggc agcgcatcag gcagccctgg
    31561 aaaaaggtga taaagcgaat acccgggccg tctacggttc cacaggattc aaaggagtga
    31621 atgcgaccaa taactgactt ggtagccccc gccgtagcgg tattttctgt tgtattacaa
    31681 ttagttaaat tactcatatc gcttcaattg gctctagtta actctggttt atggacgcat
    31741 tatggacatt cctgacaccg ggttaagagt cacagcatct tgtaagaaat ccggtgcaaa
    31801 gtgtgcgtag gtcatagttt gctgaatgtt agaatgaccc aggatgcgct gcaatgtgat
    31861 tatgttacct ccattcatta taaaatgtgt ggcaaatgta tgcctcaaaa catgtactgc
    31921 ttgtccgtca ggtaaatcgg gttttacttc cctgagagcg ttacgcactt tgtagtaact
    31981 ggcattaaaa agcctgcctg aatttttggt cttgatccgt ttaatcaggt cctgcgaaac
    32041 gggaattgtc ctgcgttttc cgtttttagt tttcataaac gtaaccatct ggttaatgat
    32101 gtgttcagct tttaaattag acacttcact ccagcgtcca ccagtagaaa ggcagaccag
    32161 agttgcattt aattcatcac catcaagcat ggataacagc cgcgtaatct cttcactgga
    32221 caaaaaagcc atttccgtaa cagcttcacg taaccgttta atctcacgga acgggttgtg
    32281 agagtggtat tcaccggcgt caattaactt ggtgaacatc ccgctcatta ttgccagatg
    32341 tcgatttacg ctggctggtt tcagaccatc gttcatcatt acaacgcgat aatcagttat
    32401 cgttttcttt gttagctggt cagctctgga cactcccatt tctgcaaatt tggcgattat
    32461 tgtcgttaaa cgcccccgtt caatatctcc acgctcatgt gattttccgt gatatatcca
    32521 ccatctgcct aacaactctg taagagttcg gcggtcggct ggtttctcca accactcttt
    32581 gttgtggtag ttaaccagta catgacgttc gaatgcttga gcttcacctt tagttttaaa
    32641 tttccgcctg atacgttttc catctgcacc ctgcggtctg acgtccactt cataacgacc
    32701 atcatcgagc tttttaatag acataaagcc ctccgatgac gctgtttact tctactactt
    32761 gaaaattaat gcaattttct ttcgtacatt tactacacac atatgctgaa taaatcgtca
    32821 gccagtcttc tggtctgagt ggtgcaaggt tgttgagtct tgcccaatgt gtgcgagagc
    32881 cggggctatt tgtccagctg ctggatctgt ttcatcaaac atgaaccagt cacgatactt
    32941 gcgaaagcgt ggatgcttaa acagcttcat acctgcttcc ataggcattt ttgattttcc
    33001 tgattcatat ccatgatatg tgtagtaatt aattccaatt aattcagcaa gttccttagt
    33061 tttaaggcgt tcggattcac ggatgagctt tagtttctca ctttgttcac ttgacataat
    33121 tttgctaatc tccaattata ttgttacttg gcaatttgtg attcgctcaa aaaggctcta
    33181 gatggctcca gttggtgaat cgcaaatctt atggaggata gcaaagtgac gacaaatgta
    33241 gaaatttctt gtccaactag cgatgaggca agtttcgaaa atgaagccaa acgtaaaagc
    33301 attcagattt cagagcgccc atctgatttg ctgtcgaaag aaggttttgc tctttacatc
    33361 ggtaagacac cacgcgcggt ggctgaaatg gctaaagcag gaaagctgcc agccttctac
    33421 atgacagacc cattaaaacc aagagggaaa gctgaattat ggattaatcg ccgtgagtgg
    33481 gacaagtacg cagcccagct agttgatgaa gctccgacag aatggcatga ctggaaaaat
    33541 cgcattagtt acagcaaatc aagacatggc cgtgcggctt aaggtggaaa ggatgaacga
    33601 gcctcgttgt attgctcagt tattgcgtaa cgaaagcccc agggcgattg acttcaccat
    33661 cacccacggt aaggggcgta agggaatcat tatccgcacc aaaaaacaga gtccggtaaa
    33721 aaaggctctg acctttctga aaagccggag ggtctggaaa tgacagtgat gacgctcaat
    33781 ctcgttgaaa aacagccagc aaccatgcgc cggataattg gtaagcatct tgccgttcct
    33841 cgctggcagg atacatgtga ttattataat cagatgatgg agcgcgaacg gttaacggtt
    33901 tgcttccatg ctcagttaaa acagcgtcac gcaacgatgc gttttgaaga aatgaacgac
    33961 gtcgaacgtg aacgattggt atgtgcaatt gatgaattgc gtggggcatt ctcaaaacgc
    34021 cgtcaggttg gcgcaagtga gtatgcatat attagttttt taacagtcag tcagcgtcgc
    34081 actttattta tgcacgcacg actgacagaa aaagaattta accagccata ctggcgaatt
    34141 aatgaagaat catgttactg gcgtgatgct ttattccgtg cattacgtga attattcagt
    34201 ctgtttgagt atgcaccgac aattctgacg tcggtaaaac cagagcaata tctgcattaa
    34261 gtaattaacc agagttttta acgcacttaa ttgtgcgggg cttctttttg cctggagaaa
    34321 gtcatgcata cagtttctga aaatcagtgc ggtaaatacg cattactgct gcaacaggcc
    34381 agaaccgaag cacaggctga tgctgcgacg cgcttttctt ctcatcttga cgccatgatt
    34441 cgccatatca caaaggcgga gttatcccgc gtggagatag tcgagctgct cagtcaggag
    34501 tcggaaaaat ttcacaatat cggattgtct cgcggggagg tgctttgatg tcctgttctc
    34561 attcagttgt attactgaat aacgccttaa aaatcgccgt tatgaaaaat ggcgatttgt
    34621 ctcttattca actttgtctt gataaagaaa aacgcgacat aagtgaatct gttatcgcga
    34681 tttatcagaa tgaattaaac ctcctgtctg atgtggtcaa tttacttgtt aaacgcgctg
    34741 tgttccacaa gcaaatttcc tccgtggatg aactgacaaa attaacgaca gaactcgcca
    34801 gttattgcgc tgatgtatcc aggaaactta acgataaaag gagctggtaa tgccggacaa
    34861 cgtagatttt attcaggaac aacaggctga attactggag cgccagatta acgcggcaag
    34921 ggtaaaacat tgcggtgctt ctgcgctggt ttgcgaagag tgtgacgcgc caatacctgc
    34981 tgcccgtcgt gcggcttatc cgtcagccac gcgttgtgtt tcctgtcagt cagtctttga
    35041 agcaaaaaac aaacattacc ggagaatggc atgagtattc gtatcgaaat tggcgaacgt
    35101 tatgtcgtta ccagtgacag ctttcagttt attctccacg agaaaaagag agcggaaagc
    35161 ggtaaaaacg ccggtcagga atggctggcg gtggttggtt attacccgaa attaagccag
    35221 ctcgtttccg gcctgatgca tcacgatatt ctgaccggaa gcgcaaagtc ttttgctgat
    35281 ttaaacgcgc aggttgagca actcagcaag cgttgttcag aggcttttgg ctcatatggc
    35341 cgttaaagcc tccgggcgtt ttgtccctcc gtcagcattt gccgcaggca ccggtaaggc
    35401 gtttaccggt gcttatgcat ggaacacgcc acgcgaggct gtcgggcgcg aaagacccct
    35461 tacacgtgac gagatgcgtc aggtgcaagg tgttttatcc acgattaacc gcctgcctta
    35521 ctttttgcgc tcgctgttta cttcacgcta tgactacatc cggcgcaata aaagcccggt
    35581 gcacgggttt tatttcctca cagccacttt tcagcgtcgt ttatggccgc gcattgagcg
    35641 cgtgaatcag cgccatgaaa tgaacaccga cgcgtcgtta ctgtttctgg cagagcgtga
    35701 ccactatgcg cgcctgccgg gaatgaatga caaggagctg aaaaaatttg ccgcccgtat
    35761 ctcatcgcag cttttcatga tgtatgagga actctgcgat gcctgggtgg atgcccatgg
    35821 cgaaaaagaa tcgctgttta cggatgaggc gcaggctcac ctgtatggtc atgttgctgg
    35881 cgctgcacgt gctttcaata tttccccgct ctactggaaa aaataccgta aagggcagat
    35941 gaccacgagg caggcatatt ctgccattgc ccgcctgttt aactatgagt ggtggattca
    36001 tcagcttaaa ggccagcgta tgcgctggca tgaggcgtta ctgattgctg tcggggaggt
    36061 caataaagac cgttctcctt atgccagtaa acatgccatt cgtgatgtgc gtgcgcgccg
    36121 ccaggcaaat ctggaatttc ttaaatcgtg tgaccttgaa aacagggaaa ccggcgagcg
    36181 catcgacctt atcagtaagg tgatgggcag tatttctaat cctgaaattc gccggatgga
    36241 gctgatgaac accattgccg gtattgagcg ttacgccgca gcagagggtg atgtggggat
    36301 gtttatcacg ctgaccgcgc cgtcaaagta tcacccgaca cgtcaggtcg gaaaaggcga
    36361 aagtaaaact gtccagctaa atcacggctg gaacgatgag gcatttaatc caaaggatgc
    36421 gcagcgttat ctctgccata tctggagcct gatgcgcacg gcattcaagg ataatgattt
    36481 acaggtctac ggtttgcgtg tcgtcgagcc acaccacgac ggaacgccgc actggcatat
    36541 gatgcttttt tgtaatccgc gccagcgtaa ccagattatc gaaatcatgc gtcgctatgc
    36601 gctcaaagag gatggcgacg aaagaggagc cgcgcgaaac cgttttcagg caaaacacct
    36661 taatcggggc ggtgctgcgg ggtatatcgc gaaatacatc tcaaaaaaca tcgacggcta
    36721 tgcactggat ggtcagctcg ataacgatac cggcaggccg ctgaaagata ctgctgcggc
    36781 tgttaccgca tgggcgtcaa cgtggcgcat cccgcaattt aaaacggttg gcctgccgac
    36841 aatgggggct taccgtgaac tacgcaaatt gcctcgcggc gtcagtattg ctgatgagtt
    36901 tgacgaacgc gtcgaggctg cacgcgctgc cgcagacaga ggtgattttg cgttgtatat
    36961 cagcgcgcag ggtggggcaa atgttccgcg cgattgtcag actgtcaggg ttgcccgtag
    37021 tccgtcggat gacgttaacg agtacgagga agaagtcgag agagtggtcg gcatttacgc
    37081 gccgcatctc ggcgcgcgtc atattcatat caccagaacg acggactggc gcattgtgcc
    37141 gaaagttccg gtcgttgagc ctttgacttt aaaaagcggc atcgccgcgc ctcggagtcc
    37201 tgtcaataac tgtggaaagc tcaccggtgg tgatacttcg tcaccggctc ccacaccttc
    37261 tgagcacgcc gcagcagtgc ttaatctggt agatgacggt gttatcgaat ggagtgaccc
    37321 ggaggtcgtg atggcgctca ggggcgcatt aaaacacgga ctgagaacac caaatcgtca
    37381 gcaaagaaac ggaatcccgt taaaaccgca tgaaattgca ccatctgcca gactgacccg
    37441 gtcagaaaga atgcaaatta cccgtatccg cgttgacctt gctcagaacg gtatcaggcc
    37501 gcagcgatgg gaccttgagg cgctggcgcg tggcgcgacc gtaaattatg acgggaaaaa
    37561 attcacgtat ccggtcgctg atgagtggag ttggttttat tgttattcag agcaataaac
    37621 atctgattta atttgttgca taatagtaaa attgc
//
LOCUS       DAJOTB010000030        36923 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJOTB010000030 DAJOTB010000000
VERSION     DAJOTB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.030"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(142..282)
                     /locus_tag="OHN28_001537"
     CDS             complement(142..282)
                     /locus_tag="OHN28_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2296951.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            378..1094
                     /gene="arcA"
                     /locus_tag="OHN28_001538"
     CDS             378..1094
                     /gene="arcA"
                     /locus_tag="OHN28_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCQ2296952.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1154..2506)
                     /gene="creD"
                     /locus_tag="OHN28_001539"
     CDS             complement(1154..2506)
                     /gene="creD"
                     /locus_tag="OHN28_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCQ2296953.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDLRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLVLLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(2564..3988)
                     /gene="creC"
                     /locus_tag="OHN28_001540"
     CDS             complement(2564..3988)
                     /gene="creC"
                     /locus_tag="OHN28_001540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCQ2296954.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3988..4677)
                     /gene="creB"
                     /locus_tag="OHN28_001541"
     CDS             complement(3988..4677)
                     /gene="creB"
                     /locus_tag="OHN28_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCQ2296955.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFGVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSSREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4690..5163)
                     /gene="creA"
                     /locus_tag="OHN28_001542"
     CDS             complement(4690..5163)
                     /gene="creA"
                     /locus_tag="OHN28_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCQ2296956.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5374..6243
                     /gene="robA"
                     /locus_tag="OHN28_001543"
     CDS             5374..6243
                     /gene="robA"
                     /locus_tag="OHN28_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCQ2296957.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6240..6887)
                     /gene="gpmB"
                     /locus_tag="OHN28_001544"
     CDS             complement(6240..6887)
                     /gene="gpmB"
                     /locus_tag="OHN28_001544"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCQ2296958.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6939..7466
                     /gene="yjjX"
                     /locus_tag="OHN28_001545"
     CDS             6939..7466
                     /gene="yjjX"
                     /locus_tag="OHN28_001545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCQ2296959.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7545..7871)
                     /gene="trpR"
                     /locus_tag="OHN28_001546"
     CDS             complement(7545..7871)
                     /gene="trpR"
                     /locus_tag="OHN28_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCQ2296960.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7961..9898)
                     /gene="sltY"
                     /locus_tag="OHN28_001547"
     CDS             complement(7961..9898)
                     /gene="sltY"
                     /locus_tag="OHN28_001547"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCQ2296961.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10105..11772
                     /gene="ettA"
                     /locus_tag="OHN28_001548"
     CDS             10105..11772
                     /gene="ettA"
                     /locus_tag="OHN28_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCQ2296962.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11893..13125)
                     /gene="nadR"
                     /locus_tag="OHN28_001549"
     CDS             complement(11893..13125)
                     /gene="nadR"
                     /locus_tag="OHN28_001549"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCQ2296963.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13146..14528)
                     /gene="radA"
                     /locus_tag="OHN28_001550"
     CDS             complement(13146..14528)
                     /gene="radA"
                     /locus_tag="OHN28_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCQ2296964.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14577..15545)
                     /gene="serB"
                     /locus_tag="OHN28_001551"
     CDS             complement(14577..15545)
                     /gene="serB"
                     /locus_tag="OHN28_001551"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCQ2296965.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMSGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15651..16295
                     /locus_tag="OHN28_001552"
     CDS             15651..16295
                     /locus_tag="OHN28_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCQ2296966.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16323..17339
                     /gene="lplA"
                     /locus_tag="OHN28_001553"
     CDS             16323..17339
                     /gene="lplA"
                     /locus_tag="OHN28_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCQ2296967.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFQEQEKELRELSAWMA
                     GAVR"
     gene            complement(17340..18671)
                     /gene="yjjJ"
                     /locus_tag="OHN28_001554"
     CDS             complement(17340..18671)
                     /gene="yjjJ"
                     /locus_tag="OHN28_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HCQ2296968.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVARENRVIRFGK
                     ARATRYVLLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTDDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     EGNYQRWITAQRPAEIPLDQKLTRYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRHLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNIS
                     KSAWLTAIPLAQQFWQTVARDPRISEAFRHIAQEMPEKIRQIEEKVTRMGG"
     gene            complement(18838..19557)
                     /gene="deoD"
                     /locus_tag="OHN28_001555"
     CDS             complement(18838..19557)
                     /gene="deoD"
                     /locus_tag="OHN28_001555"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCQ2296969.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19614..20837)
                     /gene="deoB"
                     /locus_tag="OHN28_001556"
     CDS             complement(19614..20837)
                     /gene="deoB"
                     /locus_tag="OHN28_001556"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2296970.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20889..22211)
                     /gene="deoA"
                     /locus_tag="OHN28_001557"
     CDS             complement(20889..22211)
                     /gene="deoA"
                     /locus_tag="OHN28_001557"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCQ2296971.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEHRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(22289..23068)
                     /gene="deoC"
                     /locus_tag="OHN28_001558"
     CDS             complement(22289..23068)
                     /gene="deoC"
                     /locus_tag="OHN28_001558"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCQ2296972.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            23326..24876
                     /locus_tag="OHN28_001559"
     CDS             23326..24876
                     /locus_tag="OHN28_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCQ2296973.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVIILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNVCECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLIDPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24848..25711
                     /locus_tag="OHN28_001560"
     CDS             24848..25711
                     /locus_tag="OHN28_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCQ2296974.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPACDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVERLADSLRERG
                     VSRLIFPALYL"
     gene            complement(25946..26725)
                     /locus_tag="OHN28_001561"
     CDS             complement(25946..26725)
                     /locus_tag="OHN28_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2296975.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFDVLCELRREPADEIAQALLNNTYTLFNVP"
     gene            complement(26722..27795)
                     /locus_tag="OHN28_001562"
     CDS             complement(26722..27795)
                     /locus_tag="OHN28_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCQ2296976.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27917..28078)
                     /locus_tag="OHN28_001563"
     CDS             complement(27917..28078)
                     /locus_tag="OHN28_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCQ2296977.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(28205..28810)
                     /gene="osmY"
                     /locus_tag="OHN28_001564"
     CDS             complement(28205..28810)
                     /gene="osmY"
                     /locus_tag="OHN28_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCQ2296978.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKESSVKGYAGDTATTSETKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(29203..30792)
                     /gene="prfC"
                     /locus_tag="OHN28_001565"
     CDS             complement(29203..30792)
                     /gene="prfC"
                     /locus_tag="OHN28_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCQ2296979.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30883..31560)
                     /gene="yjjG"
                     /locus_tag="OHN28_001566"
     CDS             complement(30883..31560)
                     /gene="yjjG"
                     /locus_tag="OHN28_001566"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCQ2296980.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDCSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31575..32021)
                     /gene="rimI"
                     /locus_tag="OHN28_001567"
     CDS             complement(31575..32021)
                     /gene="rimI"
                     /locus_tag="OHN28_001567"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2296981.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31990..32403)
                     /gene="holD"
                     /locus_tag="OHN28_001568"
     CDS             complement(31990..32403)
                     /gene="holD"
                     /locus_tag="OHN28_001568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCQ2296982.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32506..33537
                     /gene="rsmC"
                     /locus_tag="OHN28_001569"
     CDS             32506..33537
                     /gene="rsmC"
                     /locus_tag="OHN28_001569"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCQ2296983.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLNRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33737..33823
                     /locus_tag="OHN28_001570"
     tRNA            33737..33823
                     /locus_tag="OHN28_001570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33771..33773,aa:Leu,seq:cag)
     gene            33852..33938
                     /locus_tag="OHN28_001571"
     tRNA            33852..33938
                     /locus_tag="OHN28_001571"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33886..33888,aa:Leu,seq:cag)
     gene            33973..34059
                     /locus_tag="OHN28_001572"
     tRNA            33973..34059
                     /locus_tag="OHN28_001572"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34007..34009,aa:Leu,seq:cag)
     gene            complement(34098..34334)
                     /locus_tag="OHN28_001573"
     CDS             complement(34098..34334)
                     /locus_tag="OHN28_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCQ2296984.1"
                     /translation="MLQRTLGSGWGVLLPGLLITGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            34475..35263
                     /gene="fhuF"
                     /locus_tag="OHN28_001574"
     CDS             34475..35263
                     /gene="fhuF"
                     /locus_tag="OHN28_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCQ2296985.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAAIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTKEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(35301..35978)
                     /gene="bglJ"
                     /locus_tag="OHN28_001575"
     CDS             complement(35301..35978)
                     /gene="bglJ"
                     /locus_tag="OHN28_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCQ2296986.1"
                     /translation="MEHSRIKKRNVALIEKCVMSRIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(35936..36661)
                     /locus_tag="OHN28_001576"
     CDS             complement(35936..36661)
                     /locus_tag="OHN28_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2296987.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLASESEEPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHNFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8751 a        10093 c         9324 g         8755 t
ORIGIN      
        1 actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga tcatttcatc
       61 aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg
      121 tgtcctgccc ctcttagcct gttatattgc tgttaaaatg gttaggatga cagccgtttt
      181 tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt
      241 gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta
      301 atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg
      361 caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa
      421 cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag
      481 atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata
      541 tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg
      601 ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa
      661 tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgact attcgtgcac
      721 gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg
      781 aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg
      841 atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa
      901 acccaggtaa aattcagtcc cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga
      961 aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta
     1021 cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg
     1081 atctggaaga ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt
     1141 ttatttttca acattatttc cagatacgta actcatcgtc catggtaact tctttactgg
     1201 ctttcatttt cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc
     1261 cggccagcgc caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga
     1321 gcagtcccca catcacacca tccagcaaca acagcacgag ggtaaacaac atgctattac
     1381 gccagccttt caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg
     1441 cgattatcca tgccacagta aaaccggtat gttcagaaag cgccagcaag agcaaataaa
     1501 acatcaccaa tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga
     1561 gcgtttcaaa aacaaagaat gccataaaag tcagcgcaat cagcagaatg gcgtacttag
     1621 tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg
     1681 ggaagttttc ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa
     1741 accagctact ttgccattgc gcttgaaaac ctgactcgct aacttcccgt ttggctggta
     1801 gaaaatcacc taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac
     1861 gcccgccagg caccacagaa agatcgccgg taccgcttaa attcagcgcc acattcagct
     1921 tcaggttctg cttgcgcaag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt
     1981 gctctaaccc ggtgccgggt tcaatggtca gcgccgtacc gttaacttca ggcgctttca
     2041 ccacaccaat accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga
     2101 tatttggcgc gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat
     2161 cactgtgcca gacctgaccg gtatagatgc ctatcttgcg ttcttccaca ttctgattgc
     2221 catcaaccat caatgactca ggtaaccaaa aatgaataaa acttcgtttc cgctccacgg
     2281 ttttatcgtc ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa
     2341 cgagtttttg cggcccgctg gtactttggc gaatcacatc ttccacatcg ctacggtaat
     2401 cggcacgttc gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg
     2461 caccaaacaa ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg
     2521 caatgacagc aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt
     2581 gaagtcgaag cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt
     2641 taaacaaacg ggcgacctca ctgacaaacg ccaaccccag accgctgctt ttttgcccat
     2701 ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatcc
     2761 cactaccggt atccagcacc ttaagggtga cgtattcctg atccacttcg gcgcttagcg
     2821 ttatgcgacc gctctcggga gtaaaatcga tggcgttatc cagtaaattc cccagcgcct
     2881 gctccagtaa cgccggttca gcagcaacgt taatctcagt aggcataaca tgcaaagtga
     2941 tgtttttttc tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca
     3001 catcaacagc agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg
     3061 tttccaccag tgcctgcatt cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca
     3121 ccacttccgg cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg
     3181 ggctttttag ctcatgagtt aacgcataaa catactgctc aatatagttt ttcccttcca
     3241 gcttcacgcg catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat
     3301 cggggagagg aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa
     3361 tagagcggtt gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa
     3421 tggcgctggc ccataaaatt cgccgctcgc tacgcttaat gaccggggcc atcgccgcgt
     3481 tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa
     3541 catacatcac agaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact
     3601 gaccacgcaa cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat
     3661 ttgccgaatc aaacaatact ttgccctgcg catcggtcat atagacatga tattcgttgc
     3721 gcactttgtt aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg
     3781 cctgcgccag ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg
     3841 ccagcaacgt tgcggtgtcg attaacgtcc cctcggttgc tcttcgcacg cccggtttaa
     3901 cttctttgac aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc
     3961 ccagcaacaa ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg
     4021 atgagtatta atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt
     4081 aatgtgggta tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat
     4141 tagttgctgg cgggaccaga cgcggcccgg cgacttgagt aacgtcttca gcaataaaaa
     4201 ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg caggttcatt
     4261 caattcaaaa tgaccaatac ggataacggg agacggcgtc gagaacttct tcacccgacg
     4321 cagtaaggtg cgcactctgg cgcacacttc gcgagatgaa aacggtttag ccacgtagtc
     4381 gtcagcgcca atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa
     4441 cagtacaggt aacgccggat ggagcgccag taactggcgg cacaattcaa agccgctgat
     4501 atcaggcaaa ccaacatcga gaatcatgac atcgggaacc tgctggcgag ctttatccag
     4561 cactggcaag cctcgctcaa agacctcgac gccaaaacct tcctgttgca acatgtagac
     4621 cagcgtatcg gctatccctt gctcgtcttc tactaaccag accgtttccc gttgcataaa
     4681 tcgcctctgt tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga
     4741 accttctaca actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tagcgtcata
     4801 aaagcgcacg acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc
     4861 gccctgagct ttgccgtttt taatacgatc cgacagttca atcggcccga cttgttgaca
     4921 agaaatggcc gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt
     4981 tttcgcccgg ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttcaac
     5041 aacaattttg tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc
     5101 tgcatgagcc aatggcccca gcattatcag gcttaaagaa aggattaaat gcttgtattt
     5161 catattgtta ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata
     5221 ttgattaaga acaaaatctc aatactttta tttccgtcaa gccctaaaac atactcgact
     5281 aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc
     5341 aggtgctcgt tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac
     5401 cttttaatct ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg
     5461 aaagcaggtt attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct
     5521 attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact
     5581 gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc
     5641 cgcgcattca agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg
     5701 agcgcctttg gtattcgccc gccgctgcgt ctgggtgaat tcactatgcc agagcacaaa
     5761 tttgtcaccc tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg
     5821 gagcaaatct ctgatttccg ccatgaaatg cgctaccagt tctggcacga ttttctcggc
     5881 aacgcgccga ccattccgcc ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat
     5941 aaagacgatg aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc
     6001 tatgtactga cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat
     6061 gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg tatatggaac atgcatgcca
     6121 atgctcaacc tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat
     6181 gccaaagcgg gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt
     6241 taacgctgca gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca
     6301 accacccagc cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag
     6361 ttacgcagac gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccagg
     6421 catcccagtg caataccatg gctcaccagt agcggtcggc ttccctgcgg taagtcccgg
     6481 caagattcca gtgccgcatt aacgcgatcg ctgagctcct gcattgactc gccttcagga
     6541 atacgcccgt caacggtgcc attgaccagt tgccgacgcc agttctcttc ttcttcggtg
     6601 agtgaatcga tatttcttgt ttccagtaca cccatattta attcacgcag gcgagaatca
     6661 aatatgatgt cacagccgca ggcctgggcg atgatttccg ccgtgcgccg ggtgcgtcct
     6721 aaatcgctac tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc
     6781 gcctgttgct cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg
     6841 gcgttccact gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt
     6901 atactgctgt aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga
     6961 ccaccaatcc cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag
     7021 gatcctgcca tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca
     7081 gtgaggaaac gcgcgctggc gcacgaaata gggtagccaa tgcccgccgt ttacttcctg
     7141 aggctgattt ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg
     7201 tggtgattga aaacgccagc cagcgcggcg aggcgcgttc ggctactttg ccgttgcctg
     7261 cggtgattct ggaaaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata
     7321 ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac
     7381 tcactcgcac cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcataatg
     7441 ccgtgtatca gccattgcag gcctgattag gcgttcacgc cgcatccggc attcggtgca
     7501 cgatgcctga tgcgacgctg acgcatctta tcaggcctac aaaatcaatc gcttttcagc
     7561 aacacgtctt ccagccactg gcgcagctcg acgggcgcgg ctttcagact attagatcca
     7621 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg
     7681 ccgcgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
     7741 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
     7801 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
     7861 tgttgggcca tagtatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt
     7921 actcgctaaa gagtacgata gcatatcata aacgtgtgga tcagtaacga cgtccccatt
     7981 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag
     8041 ccagcacgtt tttcacataa ccgcgcgtct cggagaatgg aatactctcg acaaatgcca
     8101 ctgcgtcgat acgccctgcg ctgttgccaa gccaggtccg cacccgccct ggtccggcgt
     8161 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
     8221 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
     8281 cgggaataga gaacatcttc accgtatggg tcgcggtacc aggcataatc tgcatcaggc
     8341 cgctggcccc taccggtgat ttcactttcg gattccaggc actctcctga cgagcaatcg
     8401 ccatcgcata gctttgcggg atctccttac cactggtgta gcgtttgaaa agatcgttgt
     8461 aagccagcgg gaatcgctct tccagatgat cccacagctt cccggcgatc gttgcctgaa
     8521 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
     8581 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt
     8641 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct
     8701 gcggcgcttt atcaatcttc agctcatact cttcgccgat gcgttgtgca gcaaccatcg
     8761 ggtagaaacc acgctgttgc atgagttgat gcaaaatctc ttttgcttca gcttcacgtc
     8821 cgcgttccag cagtaaatcc gcctgccagt aacgccattc atctttctct ttcgcttcca
     8881 taggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
     8941 gcacacggcg ttcaataagc gaagtagatt gcgagcgcat aatcgcgtca tcgcgccatt
     9001 tcgcctgctc gtcggtgaca tcgttgccca tcaaacgcca ggcgacaata tcgcgcagct
     9061 cctgaatctg atcttcatta agctgctgtg cctgagcaag cgacgggatc atcagccgtg
     9121 cattttcagc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
     9181 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
     9241 aaatgattgc cgaagcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
     9301 ccaggccggt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
     9361 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
     9421 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
     9481 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
     9541 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
     9601 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
     9661 gattcatcag gtcatcagtg atctggcggt attccaggta gggataaagt gggtaatctt
     9721 tcagtccagg catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttaa
     9781 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
     9841 tcagcagaca gacaccggca gccaacagcc gccaggtaac gtgtttggct ttttccacaa
     9901 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
     9961 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac ggggtgatcg
    10021 actggctggg attagacagg gaaacaggct acacttcgcc tttgaaaact catcatcacg
    10081 ataaacatag aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc
    10141 aaagttgttc cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg
    10201 gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct gcgcattatg
    10261 gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt
    10321 tatctgccgc aggaaccgca gctgaacccg gaacacaccg ttcgtgagtc cattgaagaa
    10381 gcggtttctg aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtatgcc
    10441 gatccggatg ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt
    10501 caggctcacg acggtcacaa cctgaacgtt cagctggagc gtgcggcgga tgcgctgcgt
    10561 ctgccggact gggatgcgaa aatcgctaac ctttccggtg gtgagcgtcg tcgcgtggcg
    10621 ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac
    10681 ctggatgcgg aatccgtagc gtggctggag cgcttcctgc acgacttcga aggcaccgtg
    10741 gtggcgatta cccacgaccg ttacttcctc gataacgttg cgggctggat cctcgaactt
    10801 gaccgcggtg aaggtattcc gtgggaaggt aactactcct cctggctgga gcagaaagat
    10861 cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag
    10921 ctggagtggg tacgtcaggg aactaaaggc cgtcagtcaa aaggtaaagc acgtctggcg
    10981 cgctttgaag aactcaacag caccgaatat cagaaacgta acgaaaccaa cgaactgttt
    11041 attccacctg gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc
    11101 tatggtgatc gtctgctgat tgactccctg agcttctcga tcccgaaagg agcgatcgtc
    11161 gggatcatcg gtccgaacgg tgcgggtaaa tcgacgctgt tccgtatgat ctctggtcag
    11221 gaacagccgg acagcggcac catcactttg ggtgaaacag tgaaactggc gtcggttgat
    11281 cagttccgtg actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg
    11341 gatatcatga agatcggcaa caccgagatg ccgagccgcg cctacgttgg ccgctttaac
    11401 tttaaagggg ttgatcaggg taaacgcgtt ggcgaactct ccggcggtga gcgcggtcgt
    11461 ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc
    11521 aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc
    11581 tgcgcgatgg ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacattctg
    11641 gattaccagg atgaaggtaa agttgagttc ttcgaaggta actttaccga gtacgaagag
    11701 tacaagaaac gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt
    11761 attgcgaagt aatgcgtaaa atgccggatg cggcgtaaac gccttatccg gcctacaaac
    11821 caagcaattt caatacgttg tgaatctcca ggcctgataa gacgcggtaa gcgtcgcatc
    11881 aggcattggt tcttacctct gctcccccat catctcccgc accagctcca cgcagcgcag
    11941 aaaacggctg tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    12001 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    12061 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    12121 atcaatcaac gcctgcacaa acggatgctc acgcccttcg tactttttgc agaacgcctg
    12181 ggtagtgaca aaatcggtgt cgataaacgc cactttattg gcatatttca ctgcaaaatc
    12241 aatatattgc gcatgtccca gcgcgatttt atcgtagtcg gaatactgca acgcgatctc
    12301 atcgccaccg aggtgcgaaa agacataatc acgaccatat tcccacgcac tggtagtgtt
    12361 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgattcac caccgaggat
    12421 cgccacggta cgcacaaaga atggcttcac ttccgtagga atatattccc agtagcggaa
    12481 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgcttcg gatcgaccag
    12541 caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    12601 gatcaggtcc ggctgaatcc ctttttcagc cataaacttt ttgatgccgt tgctccatac
    12661 atcccagccg tgtggatatg gctccatgcc ctcttcattg aaagcatgaa tacggatatt
    12721 tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg tgggctgctg
    12781 cgacatggcg ctgtcttcga acaatgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    12841 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    12901 cgggtaaaac ttaccgaata cgacaccgat cgttttcttc tgccggggaa attcaagtcc
    12961 caaataacgg tgcaatgcct ccagcttttg tgcgctgggg cttttaattt tggcattcag
    13021 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    13081 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    13141 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    13201 tgcatccctt ccggcgtttt tttcggcacg ttagccgccg gaacaatcgc ccggcgaaaa
    13261 ccgtgtttcg ccgcttctga gattcgttcc tggccgctgg gcaccgggcg gatctcccct
    13321 gccagcccga cttcaccaaa caccaccaga tcctgcggta gcggtctgtc acgcaggctg
    13381 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt ccgtcacctt cacgccaccg
    13441 accacgttaa caaacacatc ctgatcggcc atttgtagac caccgtgacg gtgaagcacc
    13501 gccagcagga ttgccagacg gttttgctcc agcccgactg ccacgcggcg tggattcgcc
    13561 atcatcgagt gatcgacgag cgcctgaatc tccaccagca gcggacgcgt tccttcccac
    13621 actaccatca ctgagctacc ggaggtcact tcatcgccgc gacttaagaa aattgccgaa
    13681 gggttgctga cttcacgcag cccctgctcg gtcattgcaa agacacccag ctcattcacc
    13741 gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc atcgccatcc
    13801 aaaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    13861 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    13921 taagccgccg tttcacgcac ctgtgccacg ctgccaggcg atgattgtac atccgccata
    13981 tgcatcacct ggatggagtc aattaccatc agcttcggtt gctcttcttc ggcaatcagg
    14041 cagatctgct cgatgctggt ttccgacagc atattaagat tgtcagtcgg caggccaagg
    14101 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc
    14161 atctgctgcg ccagtttgca cagcgtttgc agcaacagcg tggatttccc cgcaccaggg
    14221 ttaccgccaa tcagaatggc gcttcccggc accacgccgc cgcctaatac gcggtcgaac
    14281 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatgtcgga aagtttctgg
    14341 acttttgcca ctccggcgct accggcatag ccactgagac gctcgttacg cgccaccgtt
    14401 ggcgacgctg caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    14461 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    14521 tttgccacga tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc ggacaattac
    14581 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    14641 gtgacttccg tcttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgcc
    14701 gctttgatca tcggcaggtc attggctcca tcgccaatcg ccacggtctg cgccagcggg
    14761 atttcatact cctgcgcgag gcgggtcagg gttttcgctt tatattgcgc gtcaacgatg
    14821 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    14881 gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca
    14941 atcgccactt tccagcccag cgtttccagc ttgagtacca gctgcgttaa gcctgacatc
    15001 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    15061 acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttacc
    15121 tccgccaccc tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    15181 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg tgggattttc
    15241 cccagcggtg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg cgcagtgagt
    15301 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccata
    15361 gccgcgccca gtttgctctg gtattgggtc agacgttgtt tatccagccc acgaccatac
    15421 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    15481 gaaagaggca gaccaggcca taaagagaca tcttcaggca agtcgcacca ggtaatgtta
    15541 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    15601 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    15661 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    15721 tggcgctgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccgc
    15781 aactggaaga actggcccgc accctggcgc ggcaggtgac gctaaacgtt gcaccgctga
    15841 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    15901 aaagccgcat tctcgacgca ggcgtatatg atgaacaagg cgatcttatc gcccgttctg
    15961 gcgaaagcgt cgacgtgcgc gaccggctgg cgctcgacgg caaaaaagcg ggcggctatt
    16021 ttaatcagca gattgtcgaa cccattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    16081 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    16141 ttttacgcct gatgttgctg ctctcgctgg caatcggcgt agtgctgacc cgcacgctgc
    16201 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    16261 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    16321 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    16381 tagaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    16441 atgccgacac ggtagtgatt ggtcgtgcgc agaacccgtg gaaagagtgt aatacccggc
    16501 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    16561 acgatctcgg caatacctgt tttaccttta tggctggcaa gccggagtac gataaaacta
    16621 tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcttccg
    16681 ggcgtaacga tctggtggtg aaaaccgccg aaggcgaccg caaagtctca ggctcggcct
    16741 atcgcgaaac caaagatcgt ggcttccacc acggcacttt gctgctcaat gccgacctta
    16801 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    16861 cagtgcgttc ccgcgtgacc aatctgaccg aattgctacc ggggatcacc catgagcagg
    16921 tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa
    16981 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgccaga
    17041 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    17101 ttacctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    17161 aggtgtttac cgacagtctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    17221 gctgtctgta ccgcgcggat atgctgcaac aggagtgcga agcgctgctg gttgacttcc
    17281 aggaacagga aaaagagcta cgggagttgt cggcatggat ggcaggggcg gtgcggtagt
    17341 tacccaccca tgcgggtaac tttctcttcg atttgccgga ttttttccgg catttcttgc
    17401 gcaatgtggc gaaacgcctc gctgatacgc ggatctctgg cgaccgtttg ccagaactgc
    17461 tgcgccagcg ggatcgccgt taaccatgcg cttttactga tgttaagatc aaacttcacc
    17521 tcaatggcag catcacgcag cattccggcg ctgtttggcg cataggccat cggcagcatg
    17581 tcgtagacgg gtgtcagcgc aaatggtggt tcagataaat aaaatgataa attgcctgcg
    17641 tgcatatcgc tgttggcgat aagtcgccca aacgcccaga tcacttctgt ttgcgccacg
    17701 ctctggtgag tgacaagttg ttgctcacac aaatgccgca ttgcctccgg ccacgatccc
    17761 ggagaagaga taaactcact ttgcaccgcc tccagcgaca caatgggcaa gcgaccatcg
    17821 ttacctttgc agtcaaagcg ttccgcttcc aggaatacct gcctgttact tgttacaagc
    17881 accgttgact cgatggcgtg gatcccgccg tcacgcagga tttgcgcggc aatagattca
    17941 gcaattagca ggtcccccca acgttggctg actgcggttt gctgtggcac ggtgaatttc
    18001 accaacacat gtttattgcc tgacggcgtt tgtgcatagc aggtaaattt tggctgctcg
    18061 ccgcccgcag aagaacccac aatttctcca gccagagcat cgcttgccaa ctgttcgtaa
    18121 cgggtgagtt tttgatccaa aggaatttcc gcagggcgtt gtgcagtaat ccatcgctga
    18181 taattcccct ccccgaccaa ccaaccgcca gtatattcac cgttaaatac ggtcagggcg
    18241 tagagcacat cttcttcctg ccagagacgt atatcatcag tcagattcag ttgcgcggct
    18301 aactttctgc cccacgcgcg ccctaaaaag ccctgcggtc ggagatcggt caaataccag
    18361 ggcaaaccat caaaccactg ttcgtcgcca tctgcgcctg ttaccagaca acttccctgt
    18421 ggccagcaca accggatgtc ggcgaattta tgcgcctttc cggcatcgtc cacccgccag
    18481 acgggaatac gctcaattcc gcgataagga cgcagcagta catatcgcgt tgcccgtgct
    18541 ttaccaaagc gaatcacccg attttctctg gcaacaaggc gtgagaacgt cgcctgactg
    18601 attgccagac gctggcgcaa ttccggggca gaacgcggcc cttgcagtaa aagatcggtc
    18661 agttcgctca ttaatgaata gattcgtgaa tagatacttg aatagattta ttaacatatt
    18721 tcactgaaaa gtatagcgaa aatattcaag ctggagagat aaatgaataa atactaaaaa
    18781 gccggagcag tctcccactc cggcttcaca cgacaatcgc ctttcagcaa aacacaatta
    18841 ctctttatcg cccagcagaa cggattccag cgcgattttg atcatgtcgt tgaaggtggt
    18901 ctgacgctca gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca cggtgcagat
    18961 ggtcagggct ttcgcgccaa attctgcagc gacgccgtag atacccgccg cttccatttc
    19021 cacgccgagg atgccgtatt tttccatcac gtcgaacatt tcgccgtccg gagagtagaa
    19081 caggtcagcg gagaacaggt taccaacgcg agcatcaacg cccagtgctt ttgccgcgtc
    19141 taccgcgtta cgcaccatgt cgaagtcagc gatagcggca aagtcatggt ctttaaaacg
    19201 gatgcggtta actttggaat cggtgcaggc acccataccg ataacgacgt cgcgcagttt
    19261 tacgtgcggc agaacagcgc cacaggaacc cacgcggata attttcttca cgccgaaatc
    19321 agtgatcagt tctttggtgt agatggagca ggacgggata cccataccgt ggcccattac
    19381 ggaaattttg cggcctttgt aagtaccagt gaagcccagc ataccgcgaa cgttgttcac
    19441 ttcacgggca tcttcaagga aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg
    19501 catcaaaact acgtcagcaa aatcgcccat ttctgcatta atgtgtggag tagccatcgt
    19561 tttatccttt ttgtgacata ttaaaggcgg agtcactgct ccgcccaaat ccatcagaac
    19621 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    19681 tccgcgaagg tttcacgatg acccagcgaa cctggtttca ctttcgggcc gtaaaccagt
    19741 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcgggtcaca accgtggtca
    19801 gcagtgagaa tcagaatatc gtcatcgcgc agcaaagaca tcagctccgg cagacggcgg
    19861 tcgaacagtt ccagacccgc agcataaccg gcgacgtcgc ggcggtggcc ccaggaagag
    19921 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    19981 atggtggcgt caaacagcgc gtccagcccg gtcgctttca cttttttggt gataccgcag
    20041 ttggcataga tgtctgcaat tttaccgacc gaaaccacct ggccgtgttt ttcatcaacc
    20101 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccagta
    20161 cgctggaagt taccggcttt gtcgccgata aacggacgag cgataacacg accgatgttg
    20221 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gctcgtagag tttatccagg
    20281 ccaaaggttt cttcatggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    20341 ggcttgccgg ttttcatatg ctcttcgccc agttgatcca gaatgaccgt accggaagag
    20401 tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc cagcaactct
    20461 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cggcacacca
    20521 gcaatttccc agtgaccaga cggggtgtct ttaccggatg acatttcgtg cgcccatgcg
    20581 tacgcgccga taacttcagc gttgccatcc attcccgccg gaatgaaacc ggtagaacct
    20641 tcgtgtgctt tcgccagccc aagacgggtc agatttggca gattgagcgg gcctttacga
    20701 ccgtggtcag cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg
    20761 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    20821 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcggatca
    20881 gtataccgtt attcgctgat gcggcgatag acagttggtg tgctttccgg tgctttatcg
    20941 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    21001 gcgtggataa ctgccagcgg acgctgaccg tctacctggt cgcccagacg cgccatatca
    21061 gtaaagccga cgctgtaatc gatggtgtca gatgcctgac ggcgtccgcc gcccattgca
    21121 accactgcca tccccagtgc gcgggtatcc atttcactga caaacccttc ggtatcagca
    21181 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    21241 gtcgggcctt tttgtgccgc gaccatacga ccaaagactt ctgccgcttt accgttgtcc
    21301 agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatttt tcgccagttt gccggagatc
    21361 agcatctcca cgcacagtgc catcgtgaca tcaaacagac gcgggttacg atgttcaccc
    21421 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    21481 tgattcatat cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    21541 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    21601 ttcacgtcca tcaccagcgc gtccagacct tccgccagtt tcttcgccag aatagaggcg
    21661 gtgatcagcg ggattgaatc cacggttgcg gtaatatcgc gggtcgcgta gaaacgttta
    21721 tccgccggag ccagtgagct ggtctggccg ataatcgcca cgccgacgtc tttaataatt
    21781 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    21841 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccacag
    21901 gcagcgacca ttggccccaa catcagcgaa gtcacatcgc cgacaccgcc ggtcgagtgt
    21961 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    22021 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    22081 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatgcc gttgataaag
    22141 aaacgaattt cttcatcact cagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    22201 gcgagaaaca aggtaacctc ccgtggaaaa gagacgaggt aatcggggaa ccggatgagc
    22261 atcgctgcat catccggcaa atcaagcctt agtagctgct ggcgctctta ccgtcgccgt
    22321 ggcccagagc tttcagcagg cttgccagca agctggaagc accaaagcgg tagtgacgcg
    22381 catcagccca gtcagcaccg aacagttcat ctgcaatggc gagatatttc tgcgcatctt
    22441 ccgcagtacg cacgccgcct gccggtttga aaccaacggt tttttctacg cccatatcgc
    22501 ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc attcacagcc actttaccgg
    22561 tagaggtttt gatgaagtca gcacccgctt tgatggagat ttcagacgct ttacggatca
    22621 gcgcttcgtc tttcagttcg ccggtttcaa taatcacttt cagcagtacg ttcgccgcag
    22681 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cctgccatca
    22741 gcgcgcggta cgggaacacc acgtcaactt catcggcacc gtaggcgatt gccgcacggg
    22801 tttctgccag cgcgatttcg atgtcgtcgt taccgtgtgg gaagttagtt accgtagcga
    22861 tacggatttc cggggtgccc tgctctttca gagttttgcg agcaatcggg ataaagcgag
    22921 gatagataca gatagcggcg gtattgccga ccggagtttt ggcctgatga cacagtgcga
    22981 tcactttctc atcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    23041 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    23101 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tgtacatcac
    23161 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    23221 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    23281 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    23341 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccggtact tagcccggag
    23401 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttaccc acagctgcct
    23461 gccgaagccc gccgcgcgct ggatgagggg gtaatctgcg atatgtttga aggtcatgcg
    23521 ccgtacaaac cgcgctatgt cttacccgat tacgctcgtt ttctggcgca cggttccgaa
    23581 tggctggagc tggaaggcgc gaaagacctt gatgacgcac tctctctgct gaccattctt
    23641 taccaccatg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    23701 ttgcaaccgt atgttataat tctaacacaa gacgagatcg atattcgaat aaaacgtttc
    23761 tggcgttacc tcgacagaac cctgccagac gcctttatgc atgccaatat cggtccgtct
    23821 gattcgccca ttacccgtgc aatattacgt gcagacgcag agctgaagca ggtttcaccg
    23881 aacctgacct ttatctacga tcctgatatc acccctgatg acctgctgct ggaagtggcg
    23941 aaaaacgtct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt
    24001 ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt
    24061 ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat
    24121 gacttcttta cgcgcactct accgcactac tgccaacagc agatcgccat catcgatgcg
    24181 cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa
    24241 gggttgattg atcctgaacg ttttgtgccg atgtttggca tgtatgggct ggcggaagcg
    24301 gttaacttgc tgtgtgaaaa agaagggatt gccgcacgct acggtaaaga ggccgccgca
    24361 aatgaagtgg gttatcgcat tagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg
    24421 aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggatcag ttccgatatc
    24481 ggcaccacgc cgggcgcgcg tttgccatat ggtgatgagc cagatccgat tacccatctg
    24541 caaactgtcg ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc
    24601 gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa
    24661 gccggaatgc gtgaatttac cgccaatgtc agcggtaacg atctggttcg cgttaccggt
    24721 tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc
    24781 acctggctgg gagaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg
    24841 ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga
    24901 cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg
    24961 tcacaatccg tggacgatgg ggcgctgtaa tgattgcgga gagtgcgtgc cgcagtgtcc
    25021 gcatcaggcg ttgcagattg tcgacggcaa agtggcgtgg agcgcggctg tctgcgagca
    25081 atgtgatacc tgcctgaaga tgtgtccgca acatgctacg ccaatggcgc aatccatgag
    25141 cgtggacgag gtgcttaacc atgtccgcaa agcggttctg tttattgaag ggataacggt
    25201 aagcggcggc gaagccacga cccagttgcc gtttgtggtg gcgctgttta ctgccatcaa
    25261 aaacgatccg caactgtgcc atctcacctg cctcgtggac agtaacggca tgttgagcga
    25321 aaccggctgg gaaaaattgc tcccggcgtg tgacggcgca atgctcgatc tcaaagcgtg
    25381 ggggagcgaa tgtcatcagc aattaaccgg acgcgataat cagcagatca agcgcagcat
    25441 ctgtttgctg gcagagcgcg gtaagctggc ggaactgcgt ttgctggtaa tccctggcca
    25501 ggtggattat ttgcaacaca tcgaagaact ggcggcgtta atcaagggac ttggtgatgt
    25561 tccggtacgc ctgaacgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctggcc
    25621 gagcgccacg ccggaagacg ttgagaggtt ggctgattca ttacgggagc gcggagtgag
    25681 caggttgata tttccggcgc tgtatctttg atatgccgga tgcggcgtaa acgccttatc
    25741 cggcctacgg ttcggcgcag gcctgtaggc ctgataagac gcgtcaagcg tcgcatcagg
    25801 cattgtgcgc cagctgccgg atgcggcgta aacgccttat ccggcctacg gttcggcaca
    25861 gacttgtagg catgataaga cgcgtcagcg tcgcatcagg cattgtgcgc cagctgccgg
    25921 atgcggcgta aacgccttgt ccggcctacg gcacgttaaa caacgtatag gtgttattaa
    25981 gcaacgcttg cgcaatctca tccgccggtt cccggcgtaa ctcgcaaagc acgtcaaaca
    26041 cccgcgcggc ctgttccggg cggtttgcct gcccctgaaa accattgagc ggcatatctg
    26101 gtgcgtcggt ttccagcaac aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt
    26161 tactggcgcg tggataggta atagtcccgc ctacgccaat tttgtagccc agttgcacaa
    26221 accgttcggc ctgttgcagg ctgccggaaa aaccatgaac cacgccagtg cacggtaaat
    26281 cgtggcgttt aagatgcatc gccagtttgt cgtgcgtgcg tcgtgaatgc aggatcaccg
    26341 gcagatcgta gcgtttcgcc agtttcagtt gttcgtcgag taaccactgc tgcctctcaa
    26401 attgcggatc gtctccgaag agatccagac cgatctcccc caccgccacc accttcgccg
    26461 gatgcctttc cagcgcctgc tgtagctgat caagagacac atcgctatgt ttttccaaca
    26521 taccaggatg caagcccaat gcggcataca gcggttgata tttttccgct aacgcctgca
    26581 cgcgggcaaa attttccgcc tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg
    26641 ccgcacgttg caggctggcc tcttcatcgc cactaaacgg cgggaaatca aaatggcagt
    26701 gggtgtcgat aaaacggcaa atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc
    26761 ctgcggagcg tcgctcactt cggcaaccag cgtatcgttg gcgactggcg ctggcggaat
    26821 aacgatcgac tccggcgtca ccaccggcac cagatggcgg ctcagcggcg ctttttcagt
    26881 caataattta ccgaccgttg ccagaaaata acgcccacat aaacgcccaa gtttatagtc
    26941 ttcacgcagc gccggaatcc gactgccgag cgcaatacta tgcaatggct tcggcggata
    27001 aatttcgaat atccgcagct ggcccggtgg tttctcaata aattgctgaa tctcacgata
    27061 gcttgtttca tgatgctgca ccagattgac cagcggctgc aggctactgt cacccagcca
    27121 gcgttccatg cgtttgaacc actgtggcgt gtagtacatt tgtgacggca cagtgcggat
    27181 gacgaccaac gtttttgctc cctgccttgc cgcctcttta accgggatcg catcactgat
    27241 cccaccatcc aggtagttaa taccttccag cgacactccg ctacgataaa agccaggtat
    27301 cgcactggag gcgcgaatca catccagcca gttttgttta gttggcaaaa agtaattcgg
    27361 cgcatagtca tcctgacgac aggcgcacat ataaaacgat ttgccgctgt caaataaccg
    27421 cgctgcggtg tccatttgca acggcatctg gcttgcggtg gcctccacca gccagtcgag
    27481 atcgataaga tttcctccac ggacaaagcg caatggatcg aaaaattcgc gttttgtggt
    27541 gtaacgcatg atgactttgc gcgcgtaacc gggctgattg cagatatacg ccgagaggtt
    27601 ctgcgccccg gcagatgtgc cgagataaag atcgaaagga ttaaactgcg cacgcataaa
    27661 ctcatccagc acgccagccg tgaaaattcc acgctgtccg ccgccttcgc acaccagagc
    27721 aatccgtcca ggctggaacg gccttagcga caacggcgca atattaccaa gcgttacagg
    27781 tattcgctgc cccacctctg ccttcctgtt tttgttgtga aagaatgaca aagtaacgca
    27841 atttattatt cgctaaaacc gcaaaagcca gtccgctgga ctggctttgg ataaaatttt
    27901 cttaatggtc tgaaatctag gggcgttttc ggcccatgaa caaactcacc aggaacagaa
    27961 taatcccgac gacaaagaca attttagctg cgcctgcagc agtaccggcc agaccaccaa
    28021 acccaagtgc ggcggcgatt aacgcgataa ccagaaatat gatgccccaa cgaaacataa
    28081 gcgtctcctt taccatagtt aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc
    28141 gatattttta gtgtggtgca catcgcgcct cccgacaaag ttctggaggt cgaattacga
    28201 cgaattactt agttttcaga tcatttttaa cgcttttcac accatctacc gctttggcga
    28261 tactttcagc acggtcgctc tgtgcctgag aatctacggt accggagagc tgaaccacgc
    28321 catcggtggt ttcaactttc acatgacggg aagggacgat atcgtccgcc agcagtttgg
    28381 ctttggtttc actggtggtg gcggtgtcac ccgcatagcc tttcacagag ctttctttag
    28441 cgtcgcgaac gtgcagtttg tcgctgacag aggtcacgcc ctcaacacct ttcgccactt
    28501 tcaccgcctc ttcggcctga gcctggcttt caacgaaacc gctcagggtc acgacttttt
    28561 gatcggtttt gacagagata tcggtgctct tgatgttgtc atgatccacc agggccgcct
    28621 tcactttcgc ggtgatggca ctgtcatcca tgaaattacc gactttattc atagagctat
    28681 cgactttttg ccctgcgctt tcattggtag tctgcgcgtt gttttctgcg taggcagagc
    28741 cggtcgcgac ggcagaagtc aacattacag ccagcagagt tttcgaaatc ttcagtcttg
    28801 tcatagtcat cgatttattc ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt
    28861 tgcattgctg aatagggaga gacttcaccc tctacagaag tcaatggtcg ccatcacaaa
    28921 agcgatgggt gatgaataac gaccattaca gcctctgaat cagttattaa tatcggtaga
    28981 atgacaatcg acggctttag atactgatat ctacgcattg aacggtattt aacgccgtca
    29041 gaaatgtcat tactttgtta aatatagatc acaattttga aaccgctcgg gatatcagct
    29101 aaaacataag caaaagtgaa tgttttaaga acattccgta agcggctaat aaggaaggga
    29161 aattgacagg gcgcagcggc taccgcgccc tggaggctag aattaatgct cgcgggtctg
    29221 gtggaactga acgtccggat aacgttcctg cgccaggcgc agattgacca tgctggtagc
    29281 gatgtaagcg aggttatcgc caccatcaag cgccagttgg ctttcgttct tacgcttgaa
    29341 ctcttcgaat ttcttcgcgt ccgcacattc tacccagcgg gcggtggcaa cgttgactga
    29401 ctcatacact gcttcaacgt tgtattcgct cttcaggcgc gctaccacca catcaaactg
    29461 cagcacacca accgcaccaa cgatcagatc gttgttggag atcggacgga acacctgcac
    29521 cgcgccctct tcggaaagct gtaccagccc tttgagcagc tgtttttgct tcagcggatc
    29581 tttcaggcgg atacgacgga acagttctgg cgcgaagttc ggaataccgg tgaacttcat
    29641 catctcaccc tgggtaaagg tatcgccgat ctgaatggtg ccgtggttgt gcaggccgag
    29701 aatatcgccc ggatacgctt cttcaacgtg cgaacgatcg cctgccataa aggtcagcgc
    29761 gtcggagatc actacatctt tcgcggtgcg cacctggcgc agcttcatgc ctttttcata
    29821 tttaccggat accacgcgca taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc
    29881 ctgaatttta aatacgaagc cggtaaattt atcttcgctc gcttctacgg tacgggtatc
    29941 ggtctgacgc ggcatcggcg caggcgccca ctccaccagg ccatccagca tatgatcgac
    30001 gccgaagtta cccagcgcag taccaaagaa taccggggtg atttcgcccg caaggaacag
    30061 ctctttgtcg aactcattag acgcgccttt caccagctcc agttcgtcac gcagctgctg
    30121 tgccagatct tcaccaaccg cagcgtcgag atccgggtta ttcagccctt taacaatgcg
    30181 gacttcctga atggtgtggc ctttaccgct ctgatagaga taggtttcat ctttataaag
    30241 gtggtaaacg cctttaaaca gcttgccgca gccaatcggc caggtgatcg gtgcgcagcc
    30301 gattttcagc tcgttctcaa cttcatcgag cagttccatc gggtcgcgga tatcacggtc
    30361 aagtttgttc ataaaggtga ggatcggcgt atcgcgcaga cgggtaactt ccatcagctt
    30421 acgggtacga tcttctacac cttttgcggc gtcgataacc atcagacagc agtccaccgc
    30481 cgtcagggta cgataggtat cttccgagaa gtcttcgtgc cccggggtat cgagcaagtt
    30541 aaccaggcag tcgtgatacg gaaactgcat cacagacgta gtgatggaga tcccacgctg
    30601 cttttccatc tccatccagt ccgacttagc gtgctggtta gagccacggc cttttactgt
    30661 accggcggtc tgaatggcct gtccgaacag cagcaccttc tcggtgatgg tagtcttacc
    30721 ggcatccggg tgagaaataa tggcaaaagt gcggcgcttc gccacctctt gcaaataagg
    30781 agacaacgtc ataattcttt cttcttgagt aagcggcatc gcgccgcgct tgttgaaaac
    30841 gaaaaattgc ggctatttta cccatcaacg ggggggaggc aatcagtgtt tacacaggag
    30901 ctgctccagt tcgtgcaacg aagaaacggt ccaggtgggc gcgatgcctt ctggttgctc
    30961 gcgattgtgc gcattcagcc agcaggtcgc aagcccggcg ttgatgccac cgagaatatc
    31021 ggactcagca gtgtcgccaa ccatcagcac gcgtgaacag tcaggattgc ccgcctgttc
    31081 cagcgcataa tcgaaaattt tcttattcgg tttggcaacg ccaacttctt cggaaatcac
    31141 cagcaaatcg aagtaatcac gcaggcccgt gcgttccaga cgcacctgtt gcaaggcgct
    31201 aaagccgttg gtgatgatgc cgattttggc gttgccacga atggcgttaa gcagggaaac
    31261 cgcccccggc aacggcgtac agatttccgc catcgcatta ataaaggcct cgttgagttt
    31321 acccggttcg acgttcagcc gttcggccca gctctcaaac cgcccgtgct gaagctgtaa
    31381 tgaagtgatc acgccgtttt gataatccac ccacagtggc ttgttaacgg cctgatagtc
    31441 ctgaaaatct tcagcggtaa aggtgacgct gtaatcaaga aacatccgct gcaggccggt
    31501 gaatgagtca aaggtaaaca gcgtttcatc ggcatcaaag aaaatccagt cccacttcat
    31561 tattccacct tgtcttacat actgattggt aacgccatga tgatggcgtc ttcgcgaccg
    31621 tccgtggtgg ggtagtaatt gcggcgaatc gtcgcctcgt taaagcctaa actttcgtac
    31681 agggcaatgg cggcagcatt tgaggcacgg acttccagcc atagcgtcgc cacgccgcgt
    31741 ttttccagtt cgtcgatcag atgttccagc agcgcccttc ccagtccctg acgctgatag
    31801 tcaggatcga ccgcaatatt gaacaatgta gcttcatcca gcaccacttg cgtaatcgca
    31861 aacgccgcca ttttgccgtt ttgcgttaac tgaaagttga gatagcgctc gccctggttg
    31921 ctggcaaagg ttttttcact ccacggaaag gtgtgggcgc gttgttcaat gtggaaagcc
    31981 gctggtaaat cagtcgtttc gagggaagaa atcgtgttca tatgtgcaaa tttgttgcca
    32041 taacgcggcg cgtgccgttg ggtttgcccg taattcggtg agcgccggtg atgtcacctg
    32101 agcgccttcc agtgatagcg gttcgtcagt acccaaccgc cagctgttgc agcggctgcc
    32161 ttgcggcagc atcgcgattt tttctggcgt cagttgcagc acttgatcgg ggctgacggt
    32221 taacgcacgc agaacatcgc tcaccagagg atcggtcagg gcgggaagat cgtttgccac
    32281 catcaccaga cggacgtgtg gcgggatcgc aatggcaatc tccccctgca acgcgccagg
    32341 gcgacgcagc gaccactggg taatgcccag ttgctgtaac tgccagtctc gtcgggatgt
    32401 catagcgaat cgctcctgtt gtcaggggcg caaatatagc aaattcgtcg ataccgcgcc
    32461 aacatatggc tataatcgcc gccagtatca attgaggagc attccatgtc tgcatttacc
    32521 ccggcaagtg aagtcttgct gcgtcacagt gatgatttcg aacaaagccg tattctgttt
    32581 gccggagact tacaggatga cctgcccgcg cgtttagata ccgcggccag ccgtgctcat
    32641 acccagcaat tccaccactg gcaggtgtta aaccgccaga tgggggataa cgcccgtttt
    32701 agcctggtcg ccacggcgga tgatgtcgct gattgcgata ctttgattta ctactggccg
    32761 aagaataaac cggaagccca gttccagttg atgaatttac tttctctgct tccggtgggc
    32821 acggatattt ttgtcgttgg cgagaaccgc agcggcgtgc gcagcgccga gcagatgctg
    32881 gccgattatg cgccgttgaa taaggtagac agcgcccgtc gctgcggcct ctattttggt
    32941 cgtctggaaa agcagccggt atttgatgcc aataaattct ggggcgaata cagcgtcgat
    33001 ggcttgacgg tcaaaacgct gccgggcgtg tttagccgcg acggtctgga tgtcggcagt
    33061 cagttgttgc tctcgacgct aaccccgcac acgaaaggta aagtgctgga tgtcggctgc
    33121 ggcgcaggcg ttctttccgt tgcttttgcg cgccattcac cgaagatccg cctcactctg
    33181 tgtgatgttt ctgcaccagc ggtagaagcc agtcgcgcaa cacttgctgc caacagtgtt
    33241 gaaggtgaag tctttgccag caacgtcttt tccgaggtga aaggtcgttt tgatatgatc
    33301 atctccaacc cgccgttcca cgatgggatg caaaccagcc tggatgcggc gcaaacgctg
    33361 attcgcggcg cggtgcgtca tcttaatagc ggcggcgagc tgcgaattgt agcgaacgcc
    33421 ttcctgcctt acccggacgt gctggatgag acatttggtt tccacgaagt gattgcgcaa
    33481 accgggcgct tcaaggtgta tcgcgccatt atgacccgcc aggcgaagaa aggttaagta
    33541 tctgatatct gtcgccgcat accgtgttgg atgcggcgta cacgccttat ctgaccgaca
    33601 aacgaattat ccacaatccg ctgatggaca tttttccagc aattacacct ctgtcgataa
    33661 ttaactattg acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt
    33721 taagaattga tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag
    33781 tgtccttacg gacgtggggg ttcaagtccc ccccctcgca ccaaaaacca cgttgatatt
    33841 gctcgcactg ggcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgttc
    33901 ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa cgaggcaata tcaaaaaaag
    33961 taagatgact gtgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc
    34021 cttacggacg tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcttttctt
    34081 gtctcttcac tgttttatca cccctgattc aaattcatta tcatcagcat gatgccagca
    34141 ataagcgcca ggcacgatgg tagcaaaacg taatgccgta actgtttgtg atacagcatc
    34201 accggcgtca ataccaatcc catcagaatg acaatccgcc attgatccgg cgataaatcc
    34261 gcatacatca gccctgtaat cagcaatccc ggcagcaaca ctccccagcc actgcccagc
    34321 gtacgttgca acatggtttc atctctttca ttgataatga taaccaatat catatgataa
    34381 tttttatcat ttgcaagcca gagaaatccc ttgctatcgg gtaaacctat cgataggatt
    34441 agcaatcatt atcatttaga ttactatcac gattatggcc tatcgttccg caccgctcta
    34501 tgaagatgtt atctggcgaa cgcatctcca gccgcaggat gctgggcttg cacaagccgt
    34561 gcgggcggcg atcgccgaac atcgtgaaca tttgctggag tttatccgcc tggatgaacc
    34621 cgccccactt aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct
    34681 actcgcggtc tattccgatc atatctaccg caaccaacct ttgatgatcc gcgaaaacaa
    34741 accgctgatc tcactgtggg cgcaatggta tatcggcctg atggtaccac cattaatgct
    34801 ggcgctgctg actaaggaaa aagcattaga tgtgtcgccg gaacacgtcc acgttgagtt
    34861 tcacgaaacc ggacgcgccg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac
    34921 actacattcg ccgcagcagc gaatggaaac gttaatcagc caggcgcttg ttccggttgt
    34981 gcaggcacta gaagcaacgg gagagattaa cggcaaactt atctggagca ataccggtta
    35041 tttgatcaac tggtatctga ctgagatgaa acagctgctc ggcgaggcga cagttgaatc
    35101 gctgcgccat gccctctttt ttgagaaaac gcttactacc ggtgaagata atccactctg
    35161 gcgtaccgtg gtgctacgcg acggcctgct ggtgcgccgc acttgctgcc agcgttatcg
    35221 cttaccggat gtgcagcaat gtggcgattg tacgctgaaa tgaaaacact gccggggaag
    35281 taaacccggc atcatgcgga ttaataagga tgcaacacat tacttgtttc gcaatgccgc
    35341 atacataaca gaatatctgc ggcctccaac aaccctgcat cagaactgac gcccagtttc
    35401 gacatcacat taaatttatg tgcacggatc gttttgatat tgcgtttaag ctgctcggca
    35461 atttgtgtca ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt
    35521 agcgtccggc tttggttaat gtaccactga ttgttcagtc gatcggtcgc ctgacgtaca
    35581 ccatttaatg acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat
    35641 acaccgtcca gcggtgatgg cgataatgaa ccaatcagcc gagcttcaat atcatcatcc
    35701 gcaataacta aacgccgggt acgcggaaac ttaatcgcca gttcagtcag gcaggataat
    35761 ccctctctgc gcgcacttct catggcagaa aaagaaaaaa tgaccgccgc aaatgagatc
    35821 cgcgacatgg catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggtta
    35881 cccgcaaact ttctgaataa actctcaata ccgatacgac tcatgacgca tttttctatg
    35941 agtgcgacat ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag
    36001 aaggcaagtt aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt
    36061 tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg ggcgctaata gttttattac
    36121 ttttcttaag caatgaagct atctggttga tgccccatcc tttacctaat agccgcagta
    36181 ccttgcgctc tgaaagagtg agaatgacgg agcgatagct aaaattatga gtttcagtca
    36241 ttgccggaga ggtcagcatg gcgctgatac gctctgagtg cgtattgccg gaacgtacgg
    36301 tcttgaccag attctcaatg ggttcaacat cagacaataa tgtcgccgta gggcacatga
    36361 gcaattctac tgcctgggaa taccaggagc gtgacaccat gaaaacccag tgaatttccc
    36421 gatattgtga gattaaagag taataatgct cacaaacaga acgtggctct tcactttcac
    36481 tggctaaatc agcaatgact aatccggaac gacgtaattg taataaggta aggtcctccg
    36541 cagaggcgct ggatattatt tcatattcag ggaagtgagt cctcatgacc tcttttaacc
    36601 ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat cctggcaaca
    36661 tatttcctcc acatccactg tctaaaaatc atgatggctt attcaaggta ttcattcctt
    36721 aaatatttcc agcaaattaa tagattaagc gaataaatta tccttagcat tcactttcat
    36781 atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt agcatcaaat
    36841 gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt gttagaaata
    36901 cttaatgaaa aacagaaatc tca
//
LOCUS       DAJOTB010000031        36762 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJOTB010000031 DAJOTB010000000
VERSION     DAJOTB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.031"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(127..2448)
                     /locus_tag="OHN28_001577"
     CDS             complement(127..2448)
                     /locus_tag="OHN28_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCQ2296988.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMEHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            complement(2545..3021)
                     /gene="greB"
                     /locus_tag="OHN28_001578"
     CDS             complement(2545..3021)
                     /gene="greB"
                     /locus_tag="OHN28_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCQ2296989.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3249..3968
                     /gene="ompR"
                     /locus_tag="OHN28_001579"
     CDS             3249..3968
                     /gene="ompR"
                     /locus_tag="OHN28_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCQ2296990.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3965..5317
                     /gene="envZ"
                     /locus_tag="OHN28_001580"
     CDS             3965..5317
                     /gene="envZ"
                     /locus_tag="OHN28_001580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCQ2296991.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5394..7016)
                     /gene="pckA"
                     /locus_tag="OHN28_001581"
     CDS             complement(5394..7016)
                     /gene="pckA"
                     /locus_tag="OHN28_001581"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCQ2296992.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7394..9118
                     /locus_tag="OHN28_001582"
     CDS             7394..9118
                     /locus_tag="OHN28_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCQ2296993.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGLLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9216..9413)
                     /locus_tag="OHN28_001583"
                     /pseudo
     CDS             complement(9216..9413)
                     /locus_tag="OHN28_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9421..10629
                     /locus_tag="OHN28_001584"
     CDS             9421..10629
                     /locus_tag="OHN28_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2296994.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(10752..11630)
                     /gene="hslO"
                     /locus_tag="OHN28_001585"
     CDS             complement(10752..11630)
                     /gene="hslO"
                     /locus_tag="OHN28_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCQ2296995.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(11655..12056)
                     /gene="hslR"
                     /locus_tag="OHN28_001586"
     CDS             complement(11655..12056)
                     /gene="hslR"
                     /locus_tag="OHN28_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCQ2296996.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(12067..12735)
                     /gene="yrfG"
                     /locus_tag="OHN28_001587"
     CDS             complement(12067..12735)
                     /gene="yrfG"
                     /locus_tag="OHN28_001587"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCQ2296997.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(12800..14935)
                     /gene="igaA"
                     /locus_tag="OHN28_001588"
     CDS             complement(12800..14935)
                     /gene="igaA"
                     /locus_tag="OHN28_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCQ2296998.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMLFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            15255..15815
                     /gene="nudE"
                     /locus_tag="OHN28_001589"
     CDS             15255..15815
                     /gene="nudE"
                     /locus_tag="OHN28_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCQ2296999.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(15981..18533)
                     /gene="mrcA"
                     /locus_tag="OHN28_001590"
     CDS             complement(15981..18533)
                     /gene="mrcA"
                     /locus_tag="OHN28_001590"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCQ2297000.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            18653..19432
                     /gene="hofM"
                     /locus_tag="OHN28_001591"
     CDS             18653..19432
                     /gene="hofM"
                     /locus_tag="OHN28_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCQ2297001.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            19432..19971
                     /gene="hofN"
                     /locus_tag="OHN28_001592"
     CDS             19432..19971
                     /gene="hofN"
                     /locus_tag="OHN28_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCQ2297002.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            19955..20395
                     /gene="hofO"
                     /locus_tag="OHN28_001593"
     CDS             19955..20395
                     /gene="hofO"
                     /locus_tag="OHN28_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCQ2297003.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            20385..20789
                     /gene="hofP"
                     /locus_tag="OHN28_001594"
     CDS             20385..20789
                     /gene="hofP"
                     /locus_tag="OHN28_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCQ2297004.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDVDTRRTGGKAAKSDADGG"
     gene            20701..21939
                     /gene="hofQ"
                     /locus_tag="OHN28_001595"
     CDS             20701..21939
                     /gene="hofQ"
                     /locus_tag="OHN28_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCQ2297005.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            22340..22861
                     /gene="aroK"
                     /locus_tag="OHN28_001596"
     CDS             22340..22861
                     /gene="aroK"
                     /locus_tag="OHN28_001596"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCQ2297006.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            22918..24006
                     /gene="aroB"
                     /locus_tag="OHN28_001597"
     CDS             22918..24006
                     /gene="aroB"
                     /locus_tag="OHN28_001597"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCQ2297007.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHSIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            24098..25384
                     /gene="damX"
                     /locus_tag="OHN28_001598"
     CDS             24098..25384
                     /gene="damX"
                     /locus_tag="OHN28_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCQ2297008.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            25491..26327
                     /gene="dam"
                     /locus_tag="OHN28_001599"
     CDS             25491..26327
                     /gene="dam"
                     /locus_tag="OHN28_001599"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2297009.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            26345..27022
                     /gene="rpe"
                     /locus_tag="OHN28_001600"
     CDS             26345..27022
                     /gene="rpe"
                     /locus_tag="OHN28_001600"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCQ2297010.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            27015..27773
                     /gene="gph"
                     /locus_tag="OHN28_001601"
     CDS             27015..27773
                     /gene="gph"
                     /locus_tag="OHN28_001601"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCQ2297011.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            27766..28770
                     /gene="trpS"
                     /locus_tag="OHN28_001602"
     CDS             27766..28770
                     /gene="trpS"
                     /locus_tag="OHN28_001602"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCQ2297012.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            28932..29837
                     /locus_tag="OHN28_001603"
     CDS             28932..29837
                     /locus_tag="OHN28_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297013.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            29854..30216
                     /locus_tag="OHN28_001604"
     CDS             29854..30216
                     /locus_tag="OHN28_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCQ2297014.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            30300..31463
                     /locus_tag="OHN28_001605"
     CDS             30300..31463
                     /locus_tag="OHN28_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCQ2297015.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            31464..32690
                     /locus_tag="OHN28_001606"
     CDS             31464..32690
                     /locus_tag="OHN28_001606"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2297016.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            32687..33565
                     /locus_tag="OHN28_001607"
     CDS             32687..33565
                     /locus_tag="OHN28_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCQ2297017.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFSEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            33576..33929
                     /locus_tag="OHN28_001608"
     CDS             33576..33929
                     /locus_tag="OHN28_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCQ2297018.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            33941..35245
                     /locus_tag="OHN28_001609"
     CDS             33941..35245
                     /locus_tag="OHN28_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCQ2297019.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            35257..36342
                     /locus_tag="OHN28_001610"
     CDS             35257..36342
                     /locus_tag="OHN28_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2297020.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(36450..36617)
                     /locus_tag="OHN28_001611"
     CDS             complement(36450..36617)
                     /locus_tag="OHN28_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCQ2297021.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         8562 a         9209 c        10081 g         8910 t
ORIGIN      
        1 tatgcgtttg cttgtggaat aaaaaggccg gagatattct ccggcacttt tatgtcaggc
       61 aaggcgttcg ttgccagatg tggcgtgaac gcctaatcgc gcaggtttag gggccagtcc
      121 gggcgtttaa cgttttttgc ccattgccgc cgccagcgca tccatcatcg cgctgttacc
      181 ggcaggctgc acttcacgcc cacgcggttt ggctgccggg cggttgtttt gcgggcgttc
      241 attaccgccg ccgcgacggg cgttggtttc gccaggctgc tcgtccagac gcatggtcag
      301 ggcgatacgt ttacgctgaa gatccacttc cagcactttc accttcacaa tgtcgcccgc
      361 tttcaccacg gtatgcggat cttccacaaa cttgttcgac aatgaagaga tgtgaaccag
      421 gccgtcctga tgcacgccaa tatcgacaaa cgcgccaaag ttggtgacgt tggtcaccgc
      481 accttcgagg atcatacccg gttgcaggtc gttcattgtc tcgacaccat cggcaaactg
      541 agcggtttta aattccggac gcggatcgcg acccggtttt tccagctctt tgatgatgtc
      601 ggttaccgtc ggcacaccga atttctcatc agtaaagtca gacgctttca ggttacgcag
      661 ttcgctgctg ttacccatca gatctttcag tgcctgctgt gttgctgcca gaatgcgttc
      721 caccaccgga taggcttccg ggtgaacggt agaagcgtcc agtgggttat caccgtggtt
      781 aatgcgcaag aagcccgcgc actgctcgaa ggctttcggc cccagacggc tcacttttaa
      841 cagttgctga cggttctgga actgaccgtt ctcatcgcgc caggctacga tgttttgcgc
      901 catcatgcgc gtcaggcccg ccacgcgggt taacagagga acagaagcgg tgttgagatc
      961 gacgccaacg gcgtttacgc agtcttctac tactgcatcc agtttgcgtg ccagctgcgt
     1021 ctggctgacg tcatgctgat actgacctac gccgatagat ttcggatcga ttttcaccag
     1081 ctccgccagc ggatcctgca aacgacgggc gatagacacc gcgccacgca gcgaaacgtc
     1141 gagatccggg aattcctgtg ctgccagctc ggaagccgag taaaccgacg cgccagcttc
     1201 gctgacgatc actttctgcg cggtcacttt cgggaactgc ttctgcacgt cgagatagaa
     1261 acgttcggtt tcgcgggaag ctgtaccgtt gccgatcgcc accagttcaa cgttatgctt
     1321 ttcacacagc gcagcaacgg tcatcgctgc ttttgcggcc tgcccggtat gcggataaat
     1381 ggtatcggtc gccaccagtt taccagttgc atcgactacc gccactttta ccccggtacg
     1441 cagacccgga tcgaggccca tcgttgcacg cagtccggca ggggccgcca tcagcagatc
     1501 gtgcaggtta cgggcaaaga cgttgattgc ttcatcttcc gcgcgttcgc gcacggtgcc
     1561 catcagttca gtttccagat gcatcaacac cttgatgcgc cacgtccagc tcaccacacc
     1621 tttgcgccag ctatccgccg gggcattgtt caggcgcagg ccaaggtgtt ccatgatgat
     1681 ttgctcgcaa tagctctctt tcggcggctc ttcgaactgc ggatcggcat tcagcgaaag
     1741 ctgaagtatg ccttcgttac gaccacggaa catcgccagt gcgcggtgag aaggcaccgt
     1801 tgacagtggt tcgtgatgat cgaaatagtc gcggaatttt gccccttcct cttctttacc
     1861 gctcaccacg gttgagacca gatgcgcgtt cttccacaga taatcacgca ctttcgccag
     1921 cagcgcggca tcttcggcaa accgttccat caggatatag cgcgcgccgt ccagcgcggc
     1981 tttggtatct gccacgcctt tatcggcatc aatatattgt gcagcggcga cttctggcgt
     2041 gtgtgacgga tcgctccaca gcagatcggc caacggctca agccctgctt caatggcgat
     2101 ttgcccacgg gtgcggcgtt taggtttgta gggcaggtag aggtcttcga gttcggtttt
     2161 gcttagggtg gcgttgatgg cattcgccag atcatcggtg agtttgcctt gctcggaaat
     2221 agacttgagg atcgcctgac gtctctcttc cagctcgcgc agatagctca gacgcgtttc
     2281 cagattacgc agctgcgtgt catccagacc gccggtgatt tccttacgat aacgtgcgat
     2341 aaacggcacg gtattccctt cgtcaagcag gcgaacggca gcgtcaacct gttccgggcg
     2401 cgcctgaatt tcacccgcaa taatgcggca gaacgaatca ttcatcatgg tttgagttca
     2461 tcttttcgga tcaaaaatca ggggatagtt atacggactg gctggcaaaa atgccagcca
     2521 tcggcaggag gttaagactc ttccttacgg tttcacgtac tcgatagcat taacatacca
     2581 gctcgcttcc ccggcagggg tattcaccac cgccagatcg ccgacttctt ttttcagcaa
     2641 tgcgcgggcc atcggggaat cgatagagat gtaatcttta cggccaaaaa tttcatcgta
     2701 gccgacaata cggaaacggt gagtcacacc atcgtcgttt tcaatctcca cccacgcacc
     2761 aaaaaagact ttgccttcct gctgcgggga gtaatcgacg attttgagat tttccatgca
     2821 tttagtgaga tagcgcacgc gacggtcgat ttcacgcagg cgctttttat tatactgata
     2881 gtcagcattt tcgctgcggt cgcccagact tgcggcccag gtcactttct ttgtgacctc
     2941 cgggcgttct tcacgccaga gataattaag ctcttgtttg agtttttcat acccttcccg
     3001 ggtaaccagg ggcgttttca tctcgttgat tccctttgtc tgtttgataa tgcgcacatt
     3061 gggtataacg tgatcatatc aacagaatca ataatgtttc gccgaataaa ttgtatactt
     3121 aagctgctgt ttaatatgct ttgtaacaat ttaggctgaa attcatacca gatttagctg
     3181 gtgacgaacg tgagcttttt taagaataca cgcttacaaa ttgttgcgaa cctttgggag
     3241 tacaaacaat gcaagagaac tacaagattc tggtggttga tgacgacatg cgcctgcgcg
     3301 cgctgctgga acgttatctc accgaacaag gcttccaggt tcgaagcgtc gctaatgcag
     3361 aacagatgga tcgcctgctg actcgtgaat ctttccatct tatggtactg gatttgatgt
     3421 tacctggtga agatggcttg tcgatttgcc gacgtcttcg tagccagagt aacccgatgc
     3481 cgatcattat ggtgacggcg aaaggggaag aagtggaccg tatcgttggc ctggagattg
     3541 gcgctgacga ttacattcca aaaccgttta acccgcgtga actgctggcc cgcattcgtg
     3601 cggtgctgcg tcgtcaggcg aacgaactgc caggcgcacc gtcacaggaa gaggcggtaa
     3661 ttgctttcgg taagttcaaa cttaacctcg gtacgcgcga aatgttccgc gaagacgagc
     3721 cgatgccgct caccagtggt gagtttgcgg tactgaaggc actggtcagc catccgcgtg
     3781 agccgctctc ccgcgataag ctgatgaacc ttgcccgtgg tcgtgaatat tccgcaatgg
     3841 aacgctccat cgacgtgcag atttcgcgtc tgcgccgcat ggtggaagaa gatccggcgc
     3901 atccgcgtta cattcagacc gtctggggtc tgggctacgt ctttgtaccg gacggttcta
     3961 aagcatgagg cgattgcgct tctcgccacg aagttcattt gcccgtacgt tattgctcat
     4021 cgtcaccttg ctgtttgtca gcctggtgac gacttatctg gtggtgctga acttcgcgat
     4081 tttgccgagc ctccagcagt ttaataaagt cctcgcgtac gaagtgcgta tgttgatgac
     4141 cgacaaactg caactggagg acggcacgca gttggttgtg cctcccgctt tccgtcggga
     4201 gatctaccgt gagctgggga tctctctcta ttccaacgaa gccgccgaag aggccgggct
     4261 gcgttgggcg caacactatg aattcttaag ccatcagatg gcgcagcaac tgggcggtcc
     4321 gacggaagtt cgcgtcgagg ttaacaaaag ttcgcctgtc gtctggctga aaacctggtt
     4381 gtctcccaat atctgggtac gcgtgccgct aaccgaaatt catcagggcg atttctcccc
     4441 gctgttccgt tatacgttgg cgattatgct attggcgata ggcggggcgt ggctgtttat
     4501 tcgtatccag aaccgaccgt tggtcgatct cgaacacgca gccttgcagg tgggtaaagg
     4561 gattattccg ccgccgctgc gtgaatatgg cgcttcggag gtgcgttccg ttacccgcgc
     4621 ctttaatcat atggcggctg gtgttaagca actggcggat gaccgcacgc tgctgatggc
     4681 gggggtaagc catgatctgc gcacgccgct gacgcgtatt cgcctggcaa ccgagatgat
     4741 gagcgagcag gatggctacc tggcagaatc gatcaataaa gatatcgaag agtgcaacgc
     4801 catcattgag cagtttatcg actacctgcg caccgggcag gagatgccga tggaaatggc
     4861 ggatctcaac gcagtactcg gtgaggttat tgctgccgaa agtggctatg agcgggaaat
     4921 tgaaactgcg ctttaccccg gcagcattga ggtgaaaatg cacccgctgt cgatcaaacg
     4981 cgcggtagcg aatatggtgg tcaacgccgc ccgttacggc aatggctgga tcaaagtcag
     5041 cagcggcacg gagccgaatc gcgcctggtt ccaggtagaa gatgacggtc cgggtattgc
     5101 gccggaacaa cgtaagcacc tgttccagcc gtttgttcgt ggcgacagtg cgcgcactat
     5161 tagcggcacg ggattagggc tggcgattgt gcagcgtatc gtggataacc ataacgggat
     5221 gctggagctt ggcaccagcg aacggggcgg gctttccatt cgcgcctggc tgccagtgcc
     5281 ggtaacgcgg gcgcagggca cgacaaaaga agggtaaata aaacgggagg cgaaggtgcc
     5341 tcccgttttg ctttctataa gatactggat agatattctc cagcttcaaa tcattacagc
     5401 ttcggaccag ccgctaccag cgcggcaccc gcaggggtgt cggtgtattt atcgaagttg
     5461 tcgataaaca gtttcgccag cgtttcggct ttttcctgcc actgttccgg agaagcgtag
     5521 gtgttacgcg gatcgaggat cttcgtgtct acgcccggca gttcggttgg gatcgccagg
     5581 ttaaacatcg gcagagtgaa ggtttctgcg ttatccagcg aaccgttgag gatggcgtcg
     5641 ataatagcgc gggtatcttt aatggagata cgtttgccag tgccgttcca gccggtgtta
     5701 accagatacg cctgcgcacc cgctgcctgc atacgtttca ccagcacttc tgcgtattgg
     5761 gtcgggtgca gcgacaggaa cgccgcgccg aagcaagcgg agaaggttgg cgtcggttcg
     5821 gtgatgccgc gctctgtacc agccagtttg gcggtgaagc cagagaggaa gtggtactgg
     5881 gtttgatcgg cagtcagacg agaaaccggc ggcagcacac caaaggcgtc tgctgtcagg
     5941 aaaataactt tagttgcgtg gcccgctttg gaaaccggct taacgatgtt atcgatgtga
     6001 tagatcggat aagaaacgcg ggtgttctcg gtttttgaac catcatcaaa gtcgattgtg
     6061 ccatcttcac gcacggtgac gttttccagc agcgcatcgc gacggatagc gttgtagatt
     6121 tcaggttccg cttctttcga cagcttgata gttttcgcgt agcagccgcc ttcgaagtta
     6181 aacacaccgt catcatccca gccgtgctcg tcatcgccaa tcaggcggcg tttcgggtcg
     6241 gtggaaaggg tggttttacc ggtgccggaa aggccgaaga acaccgcaac atcgcctttc
     6301 tcgccaacgt tggcggagca gtgcatagaa gcgatacctt tcagcggcag caggtagttc
     6361 atcatcgaga acatcccttt cttcatttcg ccgccgtacc aggtgccgcc aatcagctgc
     6421 atgcgctcgg tcaggttaaa cgccacgaag ttttcggagt tcagaccctg ttctttccac
     6481 tgcgggttag tgcacttcgc gccgttcata acgataaagt ctggtttgaa acctgccagc
     6541 tcttcatcgc tcgggcgaat aaacatgttt ttgacaaaat gcgcctgcca ggccacttcg
     6601 gtgatgaaac ggacggaaag acgagtatcc gggttcgcac cacagaaggc gtcgacaacg
     6661 aacagacgtt tgccggaaag ctgcctggtc accaggcctt tcagatgctg ccaggtttcc
     6721 ggagagagag gtttgttgtc gttcttacct ttgcctttgt ctgcccacca gaaagtatcg
     6781 cgagtggtat cgtcacgaac gatatactta tctttcggtg aacgaccggt gaagatcccg
     6841 gtatcgacgg caacggcacc cagattagtt aacaccccgc gctcataacc ggtcaggctc
     6901 gggtccagct cttcctgata cagcagatcg tagcttgggt tgtaaacgat atcatgtacg
     6961 tcactgatac cataagcctc gagttcttgc ggggtcaaac cattgttaac gcgcatttca
     7021 ctgctcctta gccaatatgt attgcctgaa tagtaaagtc tttttggggg tgttaaccgc
     7081 gacaaggctc atagatttac gtatctggag aaattcatat ttgacaaaaa actccgtgat
     7141 tcctgtcacg aaacggttgc tattatcgca gaaatagcat tttaggtgtg gaaaatgttc
     7201 tcaaaaggtt aaatcttggt ttatatggcg ggaatattgt gagtggaatc gcattcatga
     7261 atgaaattga tgcaaattca atcggtaaag tatcgattca ccacgctaac tttttttgat
     7321 tattttctga aaaaagcaaa atactactgc ccgatgcgat aattaaccca ccttttgatt
     7381 ctggataacc aacatggata acgttgaact ttcaccggca acacgctggg ggatgattgc
     7441 taccggatta cttcaggggc tggtttgcta cctgctgatc gcctggcttg ccggaaaaaa
     7501 tctcagttgg attgtttatg gcgtgcctgc gaccgtcgcg ttttcatccg ttttactctt
     7561 ctccgtgatc tcctttaaac agaatcgcct ttggggatgg ctggcgctgg tgtttattgc
     7621 cacgctggga atgagcggct tgctgaagtg gcaaactgat ggcatgaccc cctggagagc
     7681 tgaaaaggcg ctttgggatt ttggctgcta tctgctgctg atggcaatgt tgttgctacc
     7741 gtggatacaa caaagccttc gcatccgcaa tgacagtagc cgctatcgct acttttacca
     7801 gtcagtatgg cataacgtac ttatattact ggtgattttt ctcgccaatg gcctaacgtg
     7861 gctggtactt ttactgtgga gtgagttgtt taaacttgtt ggcatcacgt ttttcaaaac
     7921 gctctttttt gcaaccgact ggtttattta tctcacgtta ggtctggtta ccgcgctggc
     7981 ggtgatcctc gcgcgaacac agtcacgttt aatcgactct attcaaaagt tgttcacgtt
     8041 aatcgccacg gggttgctgc cgttagtatc attgctaacc ctgatgttta ttatcaccct
     8101 gccgtttacg ggcctgagcg cgatttctcg ccacatctcc gccgccgggt tgctgttgac
     8161 gctggccttt ttgcaattga tcttaatggc tattgtccgc gatccgcaaa aagcgtcact
     8221 tccctggacg gggccattgc gttgcctgat taaaaccgct ttgctggttg ctccgctgta
     8281 tgtgttcgtc gccgcctggg cgttatggct gcgggtcgct cagtacggct ggactgtcga
     8341 ccgcttgcag ggcgcgctgg cggtgctggt gttactggta tggtcgctgg ggtattttgt
     8401 cagtatcgtc tggcgtaaag ggcaaaatcc ccttgatctt cagggcaaag tgaaccttgc
     8461 ggtttcgtta ttggtgttgg taatactggt gcttcttaat tcgccggtgc tggacagtat
     8521 gcgcattagc gtgaacagtc atatggcgcg ttatcagagc ggtaaaaaca cgccagacca
     8581 ggtaactatc tacatgctcg aacagagcgg tcgctatgga cgtgcggcgc ttgagtcgct
     8641 gaaaagcgat gccgagtata tgaaagaccc gaaacgcgcg cgggatctgc tgatggcgtt
     8701 agatggagag caacatcttc aggaacaggt atcggaaaaa gtattagccg ataatgtgtt
     8761 aattgcccca ggttctggta aaccggatgc gacattctgg tcggccttaa tccaggatcg
     8821 ctataacgtg atgacctgta ttgaaaaaga cgcctgcgta ctggtcgagc aagatctgaa
     8881 tagtgatggt caggcggagc ggatcctgtt tgcttttaat gatgacagag tcattgtcta
     8941 tggctttgac tcagccagaa aagaatggga cgcgcttgat atgagtttac ttccgcgcaa
     9001 aataacgaaa gaaaaattac tcacagctgc gaaggatgga aaactgggga cgaggccgaa
     9061 agcgtggcgc gatctgacgg tagatggtga aacgctggaa ataaacttaa gtaagtgaca
     9121 acgcttttaa cggatacgac gctgtatctg accaaaataa cttgcgattt atccggtgcg
     9181 cggtaaaacc ccgtccttca gggcggggat ataagttcgc catccatttc aacccgttca
     9241 gcttcaaaat cagcacatac atttgaaaag taagtgcgta gtttttctgt cgcgtcatgg
     9301 tcaaaaatct ggcgttggta tttggcgaca aagaccaggt gaacatgcat caggaaaaca
     9361 caatgccttc cacgccggat atcggtttct tttttcatag accaaaatat aatttgacct
     9421 gtgaaacgac tacaggcatt taaattccag ttaagacccg gtggtcaaca ggagcgtgaa
     9481 atgaggcgct tcgcaggcgc atgtcgtttc gttttcaatc gtgctctggc gcttcagaat
     9541 gagaatcatg aggccggtaa taaatacatc ccttacggga aaatggcttc ctggctggtt
     9601 gagtggaaaa atgccactga aacgcaatgg cttaaagatt ctccctcaca gccattgcaa
     9661 cagtcactga aagaccttga gcgggcttac aaaaacttct tccagaagcg ggctgctttt
     9721 ccccgattca aaaagcgggg acagaatgat gcattccgct acccgcaggg cgttaagctc
     9781 gatcaggaaa acagccgtat ttttctgccg aaacttggtt ggatgcgcta ccggaatagc
     9841 cgtcaggtca cgggagttgt gaaaaatgtc actgtcagcc agtcctgcgg taagtggtac
     9901 atcagtattc agacagaaag tgaagtatca actccggttc acccttcagc atcaatggtc
     9961 gggctggatg ctggcgtggc taaactcgcc acgctgtcag atggcacagt ctttgagcct
    10021 gtaaacagtt ttcagaaaaa ccagaagacg ctggcgagac ttcagcgaca gttaagccgc
    10081 aaggtcaaat tcagcaacaa ctggcagaag cagaaacgca aaatacagcg gctgcattcc
    10141 tgtatcgcaa atatccgcag ggactacctt cacaaagtca caacgaccgt cagcaaaaac
    10201 cacgcaatga tagtcattga ggatttgaag gtcagcaaca tgtcaaagtc agcagcgggt
    10261 acggtcagcc agccggggcg caatgtccgg gcaaaatcag gtttaaaccg ttcgatactg
    10321 gatcagggct ggtatgaaat gcgccgccag cttgagcaca agcagctctg gagtggcggt
    10381 caggtgcttg ctgttccgcc agcgtacaca agccagcgtt gcgcgtgctg tggtcataca
    10441 gcgaaagaaa accgcctgtc acaaagtaaa ttcagatgcc aggtatgtgg atatacagcg
    10501 aacgccgatg taaatggcgc tcgtaacatt ttagcggcgg ggcacgccgt tcttgcctgt
    10561 ggagagatgg tgcagtcagg ccgcccgttg aagcaggaac ccaccgaaat gattcaggcg
    10621 acagcctgaa cgtagcaggg atccacgtcc ttcagggcgt ggaggatgtc aattcatagg
    10681 cctggtaagc gcagcgcatc aggccattac gtcgtaaaat ccggcagagt cctctctgcc
    10741 ggacatactc attaatgaac ttgcggatct gccggagacg cgttgttgcg gatctcggca
    10801 atatccatcg cattgaacag atagtggtta ccgcagtaat cacaatgcat gtcaatttcg
    10861 ccatcttccg ccaggatgct atcgacttct tcatcaggca gcgttttcag cgcatcggcg
    10921 caacgttcac gcgagcaggt gcatttgaac tccacatcct gcggatcgta aaccgtcacc
    10981 tcttcttcgt gatacaaacg ccacaacact tcgtttgccg gtaaagtcag cagttcttcg
    11041 gttttgatgg tttcggttag cgtcgccagg tggtcaaagt cgtcctgctg ggcattttgc
    11101 gcaggcatta cctgcaacaa cataccgcct gcagccggtt tgccgtctac gtcgccggtg
    11161 cgaataaaca ggcgcgtcgg cagctgttca gaacgcataa agtaatcttc caggcaggcc
    11221 gccagggtat caccttccag accaaccacg ccctgatagc gttcgccttc gctcggggtg
    11281 atggtgatca ccacgtaacc attgccgacc agcgttttca ggtcggcatt ttctggaatt
    11341 tcgccctgca cgcgcgccac accgcgcatc tgctggttgt tgttaccgtt aataaccgcc
    11401 agattcatcg gaccgtcgcc ttgcagctgt acggtgatat caccatcaaa cttcaacgta
    11461 gcggttaaca ggctggtcgc aaccagcagt tctgccagca cgtttttaac gggctgcgga
    11521 taatcgtgat tctcaaggat ctgttgcagg gtttccgaaa cggttaccag ttcgccgcga
    11581 acggcaaagt tttcaaacag atagcgatgt aattggtcat gttgcggcat aatcatctct
    11641 cttgcaggtg acagttattc actgtcgccg tgtttaaatc gtaacaggtc gcggcgctct
    11701 tttttgtccg ggcgtcggtc cggatgcggc atggttaagg cattaagttt acgtgccagc
    11761 gccatttttt cgcgtttctc tacactttcc gcagtctctt catacagcaa gactgcctcg
    11821 ctggcggggc gacgctgttc agtaatcgcc tttacaatca ccgtgcgttc gtcatttccc
    11881 tggcgcagag tgagcgtggc attcagctcg acgattttgc tcggcttgct gcgctgcccg
    11941 ttgtaatgca ccttaccacc ttcgatcatt tcgcgggcca gcgcgcgggt tttataaaaa
    12001 cgggcggccc atagccattt atccagtcga acctcaacag caggtttttc tttcatggcg
    12061 tctccttcac attagcgagg ggatcaggcg gcggtagtca ttcagtgacg gatggcgctg
    12121 atactgtttc tcggcaatcc cggagtcagg attagtcaca ccgaggcagt aacgaatacc
    12181 aaattgcgcg gcagcatcga gaatcgcttc gctgtcatca ataaacagcg ttctttcagc
    12241 tttaagaccc gtagcttcgg ccaccgcatg ccataaccgc tgatcctctt tcggataacc
    12301 aaatgtgtgg gtggaaagta ataaatcaag gtgtgcgtcc agaccggtat gctcaagttt
    12361 taccgccagg ttgtgcggat gcgcattggt gagcaaaatt cgctgtttac cgctggcttt
    12421 cagtgcctca agaaacggaa tggtatcttc acgcagtacg gcacgcggtc ccatctcggt
    12481 ggtcatcgca cagatatcca gacccagttg ctcactccag taatcaagac agtaccagtt
    12541 tagcgtatgc tgtacgtcgt gatattgctg gcgcatatat tccatcgctt cctgtggcgt
    12601 aactccgttt ttcgcgcccc atgtttcagg caccagcttt tgccagaaat agttatcgaa
    12661 ggcgaggtcg agcaacgtgc cgtccatatc cagcagaacg gtatctacgt cctgccaggc
    12721 aatgttgata tgcatgaggg aaatctccag agtgaagcaa tttgcgcgac agggtagcat
    12781 aacctgccgc gcaaacgtgt tattcgataa ggctttcgga aggggtgatc agttgcgggt
    12841 tcaggcagct ttcataatag gcctgaatct tcatcatgcg agtacgatga cgctggtaac
    12901 gacgccaggc ctgtacgcca ttataaatgg cgctacccag catcgtcagt agcagcagcg
    12961 tggtgctgag atagcgccac agaccggaac gatccgggat cggatgaagg ccaatatgct
    13021 gcgtaccatt ggcgtcagtg aagatttttg tgacgatacc ttcggcgtta aacggcgtat
    13081 gcatcagcat ttgtgccagt ttctggaaag cgttccactg ttcttgcggc ggatagtcgt
    13141 aaagtgatgc cgaaggccag ggctgatcaa caaaatcgct gccttcgtcg ctgacaatca
    13201 ggaacccgcc gggggccgga ctgtttagtg attgtgccgc tcgcgccgtt tcatgcgtga
    13261 taaacggcgc ggtagaggtg gccaccaggt tatccagcga ttccgcactc accgggcgta
    13321 ataacacatt cacgccatcg agtttcccgg cgttggcgcg ttttaccagc gcgtcccagt
    13381 ctttactgtt gccgagattg accagcgcat ttttcagtcg cacacagtca tctttggcag
    13441 aacacagatc cgctgtcttc agtactatgt cgccaaaatc atcaagcaat accatgccgg
    13501 atttttgaat tgctgaacgt aatgaggcgc tgacgcgaga ttcatcttcc ggtttagggt
    13561 gcagctggcg attaactgct tcggtcaatg ccgtcgcttt gttgaccaat tcagattctg
    13621 gtaatggcaa tgagcgggcg tcgttccaga tgatctgcga gcagtcaaac ggtaaaaaag
    13681 gtgagttggt tttcgcgctc caggtaccgg aagtgcgaat attacacatt cccgtaccgc
    13741 taatacgcaa tgtatcgcca acccgcacgc cagcgtcagc cagttgtttc acgctggtgg
    13801 cttcaatggt ctgcgcgcct ttcatccacg agagggtgaa tttcagcggc atatccagcg
    13861 ggatccagaa taacagcata aacagcacca gtagcgagcc cgcagcgata atcgtactgc
    13921 gcagccagtg ctgtaacgga aagtttttta cttcatcatg gagcgaaaga tatcgcccct
    13981 gacgcactac gtggcggtcg agatagatat cgatatcggt ttgttggccg agatcctgag
    14041 caatgtaggg ctgccagtgt gcgggataga ccaggtcgat aataccgagt gaaatattgt
    14101 tgatctgttc ctgatcgttt tcgccaaaca atccccaacg acggggtgta ccgcgcagac
    14161 aatgaatttc ccgcagggag gattttgccg ggggcgcgaa tagcccccac agacctgcgc
    14221 ccagcagcag taacgcgccg cctgccagcc acggaacaaa gacatccggg gtaatcaggc
    14281 agaaaaagag catcaggaag gaggcgacaa tcagcaacgc ttcgcgcagc ccgcgcggac
    14341 gactcagcgc atattcttca tgcgtttctt tgcgaatatt gagcagctct atttgctcgc
    14401 tctcttcccc gcgaatcgac gcctgcgttg acggaacagg ttgcaaggca tagccgcgaa
    14461 tttcctgcat gtactcctgc agcgtatgac cgttgagtga aataaccagc ggcagcgaat
    14521 cggtatgaat cagttcaacg gtattctcat cgttgatgta ctgttcccag aaagggggca
    14581 ggtggacttc taccgaatcg aggtagtaac gccatttatt cggatcgtcg gtagagatgc
    14641 cataacgcgt gatagcgtgt gtcagcatca tgacgttgtt gctttcggca ttcagcgcca
    14701 gagagatcgg tgccgcgctg gctcccgttg ggccaggcac ctgtaatacc tgcgtcaggc
    14761 tctcaagata attttcaacg gcgctacgtt cttcagatgt gagtttacgc gtttgcgcat
    14821 ccgcgaaggc gttggtccag ggcagctgac gccgtctgga tcgcactttt atcagccatc
    14881 ccgcaagtag tgagcaggcc agcaaagcag ctaaaaaaat cacaatggtg ctcatgcttt
    14941 ccccatctta cttagtctgt caggcgtggt cagtcgcacc ggacacaata catgattctg
    15001 cggttggcta tcggcaagcg tggagtttaa cttgagcatc attaagcaca tcacagtaac
    15061 cgctgatcat agcaaaccct ctgaaggcgg aatatcagca aattcctgga gttatttcaa
    15121 cctattgact tttaatttga tttgaggacg attatttatt aagttacata aatgtaaata
    15181 acgcgcaatt cacattgccg aaagcgtgtc cgatatcgca caataaccgc tgatttcaca
    15241 gcatagatcc cacgatgagc aaatcattac aaaaacccac cattctgaat gttgaaactg
    15301 tagcccgttc ccgactgttt accgtcgaaa gcgtggatct ggagttcagc aatggcgtgc
    15361 ggcgtgttta tgaacgaatg cgtccaacca accgggaagc agtgatgatt gtgccgattg
    15421 tggacgatca cctgatcctg attcgcgaat acgcagtggg aactgaatcc tacgaattag
    15481 gtttttcgaa aggattaatt gatccgggtg aaagcgtctt tgaagccgct aaccgcgagc
    15541 taaaagaaga ggttggattt ggtgcgaacg atctgacttt tttgaagaag ctcagcatgg
    15601 caccgtctta cttttccagc aaaatgaata tcgtggtagc gcaagatctc tacccggaat
    15661 cactggaagg cgatgagcca gagccgttac cacaggtgcg ctggccgctg gcgcatatga
    15721 tggatttgct ggaagcccct gacttcaacg aagcgcgtaa tgtcagcgcg ctgttcctcg
    15781 tgcgcgaatg gttgaaaggg caggggcgag tgtaaatgcg tgaagtttac ctgatgcata
    15841 atgcttatca ggcttacgaa gttcctgcaa tttattgaat tttcaatttt ttgttggccg
    15901 gataacgcgt tcacccgcat ccggcataaa caagtgcact ttgtcagcaa actgaaaaag
    15961 gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct cgccattatc gataatggtc
    16021 gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg
    16081 cggctgttgc caccattagc taattgcccg gtgctgcgat cgatattcac cgtcacaata
    16141 cccggtggcg gcgtcagcgg ctgttccggt acaccttcaa gaacggcttt catataagcg
    16201 tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg atctttaatc
    16261 gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc aatccagacc
    16321 gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact gttagtggta
    16381 cccgtttttc cgcctatatc atgccgttgt aaatcacgac ctgctcgcca gccagtaccc
    16441 tgccagcctg gttcaccaaa aatattggtg ttcaaggcac tcttaatcag gaatgccagc
    16501 ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccgg tcttcgccac taacgcctga
    16561 tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg ggagatagcg
    16621 acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc gtaaatcacc
    16681 ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaattac gccgccctga
    16741 tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt cgccatgacc
    16801 gcgtagccgc gcgccacctg cattggggtg aaggaagctg aacccagcgc cagcgattcg
    16861 gtgtggacaa tgttttgtgc cgggaagccg aagcgttgca gatattctgc agcgtagtcg
    16921 acgcccatcg cccgcattgc gcgtaccatc accacgtttt tcgactgacc cagcccctga
    16981 cgtaagcgaa ttggaccggc atattgcggt ggtgagttct tcggctgcca gtcagaaccg
    17041 gcacctgcat cccagcggga aattggcaca tcgttcaaca tacttgccag cgtcagacct
    17101 ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg acgcagtgcc
    17161 tgagtggctc ggttaaactt gctctgattg aaatcaaagc caccgaccag cgccataacg
    17221 gcaccgtttt gcggattgat cgacaccagc gccgagttca cttccggcac ttgcgccagc
    17281 caccatgcat cgtcaacttg acgaacccag atttgctgac ccgtttgcag aacatcggtc
    17341 actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc ccagcgaaca
    17401 ccttccatac tcaatacgac ggtcgacccg tctgccagca tcgccgttgc ttcctgagga
    17461 ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc cttcagcgta
    17521 tcggtaatct tgttgttatc ccacgccgat tcgcccactt tccacagcac atttgccggg
    17581 ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc ctgctgtgcg
    17641 gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc ataggcactt
    17701 tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtatgg cgcagagaaa
    17761 gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg atcgaactgt
    17821 tgttgggtga tatacccttc atccagcatc cgcgacagca cgacgttacg ccgcgcgacg
    17881 gcacgatcca ttgagtagag cgggttgaag gtggaaggcg ctttcggcag cccggctatc
    17941 accgccattt cgttcaacgt cagttggtcg acagttttac cgaaatagac ttgtgccgca
    18001 gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag ctcgaggatc
    18061 tcgtctttcg ttagcaactg ttcaatgcga atcgcgagga agacttcctt aatcttgcgc
    18121 atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt aatggtactc
    18181 gccccttgtg acgcgtgacc ggagaacagc gccacgcttg ccgcacggaa gatccccacc
    18241 gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc
    18301 atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc gtattgagca
    18361 atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac atcttttaat
    18421 gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat cgagcctgct
    18481 cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt cactggaaat
    18541 ttcccattta gtttcatttg ggcagtttat aaacaaacgc gcggtagtat aaaggcaagc
    18601 cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca caatggcatt
    18661 taagaactgg caaattggtt tgcatttaca acagcaagaa gcggtagcgg ttgctatcgt
    18721 gcggagcgca aaagaatgct tattgcaccg ctggtggcgg ttgccactgg agaacgacat
    18781 tatcaaagat gggcggattg ttgatgtgca gcggctggct aacacgttgt taccgtggag
    18841 tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc gcacattaca
    18901 gcggtcattt ccacgcccgt cgatgtccct cggtgagcgg gagcaaatgg cctggctgtc
    18961 cgggacgatg gcccgtgaac tggatatgga tccggactcc ctgcgcttcg attacagcga
    19021 agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct
    19081 gcttactctg gcagaaaggt tgcgtgttca tgttagcgcg atcactccgg atgccagtgc
    19141 attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa
    19201 cgaacagtgg ctgtgggcga cgcgctatag ctgggggcgc aaactggcgg tggggatgac
    19261 tagcgcgaag gagctggcgg cagcgttatc tgttgatccc gaaagcgtcg cgctatgtgg
    19321 cgaaggcgga tttgacccct gggaggcggt ttctgttcgt cagccgccgc taccgccacc
    19381 aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggtgtact gatgaacccg
    19441 ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg tttctggttg
    19501 ctgatgttcg ttgcgccttt gctgctggcc gtcgggataa cgctaatact gcgtctgaca
    19561 agcaatgccg aagcacgcgt aaacgccgtt ttgcttcagg cggaacaaca actcacccgc
    19621 agcttacaga taacgaaacc acgtttgctg gaacgccagc agttacgcga acagcgtttg
    19681 caacgtcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga agcactagcg
    19741 gcgcttttac ccgaacatgc ctggctgaca acgataagct ggcagcaggg aacgctggag
    19801 atcaaggggc ttacaacaag cattaccgcg ttaaacgcgc tggaaacgtc actccgccag
    19861 gatgcttctt ttcatctcaa tcagcggggg gccacgcagc aggatgcgca gggacgctgg
    19921 caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg actggtggtt
    19981 cgccacatca ctccgcctcc gccagctttg ctgggcagtc tggttgctgg tgttagttac
    20041 gctcattttt ctgtcatcga cacaccagga agagcgcgac gcattaattc gactacgggc
    20101 gagtcatcac cagcagtggg ctgcactgta tcgcctggta gacgccactc ccttcagcga
    20161 agaaaaaacg ctgccctttt cgccactgga ttttcagtta tccggcgcgc aactggtttc
    20221 ctggcatcca tccgcgcagg gtggtgagtt ggcgttgaaa acgctgtggg aagcagtgcc
    20281 gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt cgttaagcgt
    20341 ggaaggtgat gaccttttgt tcacgctaca actggagacg ccgcatgagg gttaaacgct
    20401 ggttgttggc aggtattgca ttgtgccttt taaccggtat gcgtgaccct tttaaaccgc
    20461 cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg atggtagggc
    20521 gaggcgagcg catcatcggt gtaataaaag atgggcaaaa aaaatggcga cgggtgcagc
    20581 aaaacgatgt gctggaaaac ggctggacga tcttacagct gacgccagac gcactaacgc
    20641 tgggaaccgg gacaaactgc gaaccgccac actggttgtg gcaacggcaa ggagatacaa
    20701 atgaagcaat ggatagccgc actactgttg atgttgatac ccggcgtaca ggcggcaaag
    20761 ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt gcaggcgctg
    20821 gctgaacagg agaagttgaa cctggtggtt tcgccagacg tcagcggtac ggtgtcgtta
    20881 catttaactg acgttccctg gaagcaggca ctacaaactg tagtgaaaag cgccggattg
    20941 ataacgcgcc aggagggcaa cattctctcg gtgcattccg ttgcctggca gaatgacaat
    21001 atcgcccgcc aggaggcgga gcagacgcgg gcgcaggcaa atctgccgct ggaaaatcgc
    21061 aatattactc tgcaatacgc cgacgccgga gagctggcga aagcggggga gaagctactg
    21121 agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct gcgagataac
    21181 aaaacggcgt taagcacgct tgaacagtgg gtatcgcaaa tggatctgcc ggtcgggcag
    21241 gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga gttaggtgtg
    21301 aaatggacgc tggccgatgc gcaacaagct ggtggcgttg ggcaagtcac cacgcttggc
    21361 agcgacctct ccgtagcgac ggcgacaacg catatcggtt ttaacattgg acgcatcaac
    21421 ggacgtttac tggatcttga gctttccgcg ctcgaacaaa aacagcagct ggatattatc
    21481 gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca ggggagcgaa
    21541 attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt taaagaggcc
    21601 gtcctgggga tggaagtcac gcccacggtg ttacaaaaag gtcgcatccg gctgaaatta
    21661 cacatcagcc agaacgttcc ggggcaggtg ctacagcagg cagatggcga agtgctggcg
    21721 attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac gttggcgctg
    21781 ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt gcttggcgac
    21841 attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg acgcgagtta
    21901 gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa agcggtaatg
    21961 tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca tacaaggagt
    22021 accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat ttatcattca
    22081 ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg ccagagcggt
    22141 ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg atttattcag
    22201 ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa tatcttatga
    22261 ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat taacgaatag
    22321 tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg ggcctatggg
    22381 tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg aattttacga
    22441 ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt tcgatttaga
    22501 aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga ccgagaaaca
    22561 gggtattgtg ctggctactg gcggcggctc tgtgaaatcc cgtgaaacgc gtaaccgtct
    22621 ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac ttgcacgcac
    22681 gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccgcgtg aagttctgga
    22741 agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga ccattcgtac
    22801 tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg aaagcaacta
    22861 attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg tcgcgttatg
    22921 gagaggattg tcgttactct cggggaacgt agttacccaa ttaccatcgc atctggtttg
    22981 tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat gttggtcacc
    23041 aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga acaggcgggt
    23101 gttaacgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct ggctgtactc
    23161 gataccgtct ttacggcatt gttacaaaag ccgcatggtc gcgatactac tctggtggcg
    23221 cttggcggcg gcgtagtggg tgatctgacc ggcttcgcgg cggcgagtta tcagcgcggt
    23281 gtacgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc cgttggcggc
    23341 aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta ccagcccgct
    23401 tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt agcgtcgggg
    23461 ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa ctggctggaa
    23521 gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg tattcgccgt
    23581 tgttgtgagc tgaaagcaga agttgtcgcc gccgacgagc gcgaaaccgg gttacgtgct
    23641 ttactgaatc tgggacacac ctttggtcat tccattgaag ctgaaatggg gtatggcaat
    23701 tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac gtcggaacgt
    23761 ctcgggcggt ttagttctgc cgaaacgcag cgtattataa ccctgctcac gcgggctggg
    23821 ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca tatgctgcgt
    23881 gacaagaaag tccttgcggg agagatgcgc ttaattcttc cgttggcaat tggtaagagt
    23941 gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga ttgtcaatca
    24001 gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc aggttaattg
    24061 caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca aaccagaaga
    24121 cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat cttctgaacg
    24181 ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg aacttgatga
    24241 cactgacgat cgccgtccga ctcgtgcgca aaaagcgcgt aatgaggaac cggaaatcga
    24301 agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag agcgtcgtcc
    24361 gcgtaagcgt aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga tgatgggtgt
    24421 cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa aagccccctc
    24481 gacctcttcc agcgatcaaa ctgcgtctgg cgagaagagt attgatcttg caggcaatgc
    24541 gaccgatcag gccaatggcg tgcagccagc gccgggaacc acgtctgcgg aaaatactca
    24601 gcaggatgtt tctctgccgc cgatctcttc tacgccgact caagggcaaa ccccggcggc
    24661 aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc tgacccagcc
    24721 acaaaatcag caacagttga acaatgtggc ggtcaattct acattgccga ccgaacccgc
    24781 aacggttgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa cgcaaaccgc
    24841 tgaacgtccg gccactacgc gcccagctcg tcagcaggcg gtgattgaac cgaaaaaacc
    24901 gcaagcaacc gtgaaaacgg aaccgaagcc agtcgcacag acgccgaagc gaactgaacc
    24961 agctgcccct gtggcgagca caaaggcacc ggctgcgact tctacgccag caccaaaaga
    25021 gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg caacaccagc
    25081 cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt ccagccatta
    25141 cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg cgaagaaaga
    25201 gaatctgaaa aactacgttg tctatgaaac gacgcgtaat ggtcagccgt ggtatgtcct
    25261 ggtttctggc gtgtatgctt cgaaagaaga ggcgaaaaaa gcggtatcta cattgccagc
    25321 agatgtccag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg ccgatctgaa
    25381 gtaatcaagg ttatctcccg caatggttta tcattgcggg agttgcctga agcgctggat
    25441 gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc atgaagaaaa
    25501 atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat attaaacggc
    25561 atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg gtgtttctca
    25621 acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc agtctctata
    25681 acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagcta tttgttcccg
    25741 aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa agccaggatc
    25801 cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac ggcctgtgtc
    25861 gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa ccctatttcc
    25921 cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc tattgtgagt
    25981 cttacgccga tagcatggcg cgcgcagatg atgcatctgt cgtctattgc gatccgcctt
    26041 atgcgccgct gtctgcgact gccaacttta cggcgtatca cacaaacagt tttacgcttg
    26101 aacaacaagc gcatctggcg gagatcgctg aaggtctggt tgatcgccat attccagtgc
    26161 tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca aaattgcatg
    26221 tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag gtggacgaac
    26281 tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc tcaaggagaa
    26341 gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg cccgcctggg
    26401 tgaagatacc gcaaaagccc tggcagcggg tgcagatgtc gtgcattttg acgtcatgga
    26461 taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct tgcgtaacta
    26521 cggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc gcattgtgcc
    26581 tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct ccgagcatgt
    26641 tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg tatttaaccc
    26701 ggcgacacca ttgagctatc tggattatgt aatggacaag ctggatgtga tcctgctgat
    26761 gtctgttaac cctggtttcg gcggtcagtc tttcattcct caaacactgg ataaactgcg
    26821 cgaagtacgt cgtcgtatcg acgagtctgg ctttgacatt cgactggaag tggacggtgg
    26881 cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt ttgttgctgg
    26941 ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc gcagtgaact
    27001 ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt gatctcgacg
    27061 gtacgctggt tgacagtgca cccggtcttg ctgctgcggt agatatggcg ctgtatgcgc
    27121 tggagttgcc tgtcgcaggt gaagaacgcg ttattacctg gattggtaac ggcgcagata
    27181 ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactctg cgtaaaacaa
    27241 tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt attctgcgta
    27301 aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg ttcccgcatg
    27361 ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg gtcaccaaca
    27421 aaccgacgcc gttcgtcgcg ccgctgctcg aagccttaga tatcgccaaa tacttcagcg
    27481 tggtgattgg tggtgatgat gtgcaaaaca aaaaaccgca tccggacccg ctgttactgg
    27541 tggctgaacg gatgggcatt gctccacaac agatgctgtt tgttggcgac tcacgcaatg
    27601 atattcaggc agcaaaagcg gcaggttgcc catcagttgg cttaacctac ggatataact
    27661 acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata aatgaccttc
    27721 tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac taagcccatc
    27781 gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat gggtgcgctg
    27841 cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt tgaccaacat
    27901 gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga tacgctggcg
    27961 ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca gtcccacgtg
    28021 ccggaacatg cgcagttagg ctgggcattg aactgctata cctacttcgg cgaactgagc
    28081 cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa cgctggtctg
    28141 tttgactatc cggtgctgat ggcagcggac atcctgctgt atcaaacaaa tctggtaccg
    28201 gtgggtgaag accagaaaca gcacctggaa ctgagccgcg atatcgccca gcgtttcaac
    28261 gcgctgtacg gcgatatctt taaggtgcca gagccgttta ttccgaaatc tggcgcgcgc
    28321 gtaatgtcgc tgctggagcc gaccaagaaa atgtccaagt ctgacgataa ccgcaataac
    28381 gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg cgcggtcact
    28441 gactccgacg agccgccagt agttcgctac gatgtgcaga acaaagcggg cgtttccaac
    28501 ctgctggata tcctttctgc ggtaacgggc cagagcatcc cggaactgga aaaacagttc
    28561 gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc cggtatgctg
    28621 actgaattgc aggaacgtta ccaccgtttc cgcaacgatg aagccttcct gcaacaggtg
    28681 atgaaagacg gcgcagaaaa agccagcgca cacgcttccc gtacgctaaa agcggtgtac
    28741 gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa aaccggggaa
    28801 acccggtttt tttatcctca tttgcaataa tccgaaaaaa tgtgaagcgc ctcgccgttt
    28861 ccacatctcg ctgttgcgcc ttatcttcaa attcctgaag atataaacat tcattttatt
    28921 gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgttatc acgacactgg
    28981 cccgttattt gttgggtgaa aagtgcggta atcgactgaa aaccatagat gagctggcaa
    29041 ctgaatgccg ttcatcggtt ggcctgacgc aggccgcgtt gaaaacgctg gaatcaagcg
    29101 gagcgatacg gattgaacgc cgtgggcgca atggcagtta tctggttgag atggataaca
    29161 aagcattgct gactcatgtg gatatcaaca acgtggtatg tgcgatgccc ttgccctata
    29221 cccgtttgta cgaaggcctg gcgagcggat tgaaagccca gtttgatggc attccttttt
    29281 actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac ggcgtgtatg
    29341 acatggcggt ggtttcgcga ctggcggcgg aaagttatct cacgcaaaaa ggattatgcc
    29401 tcgcgctgga gttgggaccg catacctacg ttggcgagca ccagttgatt tgccgtaaag
    29461 gcgagtccgc aaacgtgaag cgcgtggggc tggataaccg ttcggcggat cagaaaatca
    29521 tgaccgatgt cttttttggc gatagtgatg tggaacgagt cgatctctct tatcacgaga
    29581 gtttacaacg cattgttaaa ggcgatgttg acgcggttat ctggaacgta gtggcggaaa
    29641 acgaactgac catgctggga ttagaagcga cgccgctcac agacgatccg cgatttttac
    29701 aggccaccga agccgtggtc ctgacgcgag tcgatgatta cccaatgcaa caactgctgc
    29761 gtgcagttgt tgataaacat gccctgcttg ctcatcaaca gcgagtagtc agtggggaac
    29821 aggaacccag ttattaaacg aaaggcatct gatatggaaa ccagactcaa cctgctttgc
    29881 gatgcaggcg ttattgataa ggacatctgc aaaggcatga tgcaggtcgt caatgtactg
    29941 gaaacagagt gccatctacc ggtgcgcagt gagcaaggaa cgatggcgat gacacatatg
    30001 gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga tgacgaattg
    30061 ctggcggaac tggcgcaatc cagccactgg caagccgttg tgcaattgca tcaggtattg
    30121 ttgaaggaat ttgcactgga agttaacccg tgtgaagaag gctatttgct ggccaacctt
    30181 tatggattat ggatggctgc taacgaagag gtttgaagct cccgataaat ccacgtcgta
    30241 caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga ggcaacaaga
    30301 tgtttgtaga agcattgaaa cgccagaacc cggcgctgat ttccgccgca ctaagtttgt
    30361 ggcagcaggg caagatcgcc ccggacagtt gggtgatcga cgtggatcaa gttctggaaa
    30421 acggcaagcg gctgattgag acggcgcggc tttacggcat tgaactgtat ctgatgacca
    30481 aacaattcgg tcgtaatccg tggctggcag aaaaactgct ggctctgggc tacagcggca
    30541 ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgccggtttg ccggtagcgc
    30601 atcaggggca tctggtacaa atcccttgtc atcaggtttc tgacgccgtt gaacagggaa
    30661 ccgacgtcat cactgtgttt accctcgaca aagcgcggga aatttctgcg gcagcggtga
    30721 aggccggacg tgtgcagtct gtgctgctca aagtttatag cgacgatgat tttctttatc
    30781 cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtggttgct gaaatccaga
    30841 atctgccggg gctacatttg gcgggactta cccatttccc ttgcctgctt tgggatgagg
    30901 ctgccgggaa agttttgccg acaccgaatc ttcacacgct ggtacaagca cgggatcaac
    30961 tggcgaaatc tggtattgcg attgagcaac tgaacgcgcc ttcagcgacc agctgcacgt
    31021 cgctgccatt gctggtggaa tacggtgtga ctcataccga acccggtcat gcgctgacgg
    31081 gcactattcc ggcaaaccag cggggcgatc agcctgaacg tatcgcgatg ctctggttaa
    31141 gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt tactaccgtc
    31201 gtggtcacgc gcaacatgcg ctggtgttta cgccagaaaa tcaaagaatt actgaaacat
    31261 atctcaacgc cgtggatgac agcagtatcg actacaccct gccgctggca ggcgagcatc
    31321 cggtaagcag tgcagtggtg ctctgttttc gcacgcagat ttttatcacc cgtagcgatg
    31381 tggtgttggt gtccggtatt caccatggcg aaccagaaat agttggtcgt tatgacagcc
    31441 tcggcaaccc tctggaggcg taaatggcgc gatttgtggt gttagtgatt gatagctttg
    31501 gcgtaggggc aatgaaagat gtcacgttgg tgcgtccgca ggatgccggg gcgaatacct
    31561 gtggtcacat cctgagccag ttgccgcatt tgcagctacc aacgctggag acgctggggc
    31621 taatcaacgc attgggttat gcgcctggcg atatgcagcc gtcagactcc gcaacctggg
    31681 gcgtggcaga gctgcaacat gaaggtggcg atacctttat ggggcatcag gaaattttag
    31741 gtacgcgccc attaccgccg ctgcggatgc cctttcgtga tgtgattgac cgtgttgagc
    31801 aggcattagt ttccgctggc tggcaggttg agcgtcgtgg cgatgatctg caatttctgt
    31861 gggtcaatca ggcggttgcg attggcgata atctcgaggc ggatttaggt caggtctata
    31921 acatcaccgc caatctctct gtgatctctt ttgacgacgc aatcaaaatg ggtcgtatcg
    31981 tgcgtgagca ggtacaggtc ggtcgggtca ttacatttgg tggcctgtta accgacagcc
    32041 agtgcatcct cgatgccgca gaaagcaaag aagggcgctt tattggcatc aatgcgccgc
    32101 gttctggcgc ctatgacaac ggtttccagg tcgtacatat gggctacggt gtcgatgaaa
    32161 aagtgcaggt gccgcaaaaa ctgtatgaag cgggcgtgcc aaccgtgctg gtgggtaagg
    32221 tggcagatat cgtcagcaat ccttatggcg tgagctggca aaacctggtg gatagccagc
    32281 ggattatgga tatcaccctc aacgaattta acacctatcc gacggcgttt atttgtacca
    32341 atatccagga aaccgacctc gctggtcatg cagaagacgt cgcacgttat gccgaacgtt
    32401 tgcaggtcgt tgaccgtaac cttgcccggc ttgttgaggc gatgcagcca gatgattgcc
    32461 tggtcgtgat ggcggatcac ggtaacgatc cgaccatcgg tcacagccac catacccgcg
    32521 aagtggtgcc agtgctggtt tatcagcaag gactggtcgc tacgcagctc ggtgtgcgca
    32581 ccacgctttc tgatgtgggg gctaccgtgt gtgaattttt ccgtgcgcca ccgccacaaa
    32641 atggtcgctc ttttctttcc tccttccggt ttgcaggaga caccctatga gttttgatcc
    32701 gacgggctac accctcgccc atgagcatct gcatattgat ctttccggct ttaaaaacaa
    32761 cgtggactgc cgccttgatc agtatgcgtt catttgccag gagatgaacg acctgatggc
    32821 ccggggcgtg cgtaatgtga ttgagatgac caaccgttac atggggcgca atgcgcaatt
    32881 tatgctcgat gtaatgcgcg aaaccgggat caacgtggtg ggctgtaccg gttattacca
    32941 ggacgcgttt ttctcggaac atgtggcgac ccgcagcgtg caggaactgg cgcaggagat
    33001 ggtcgatgaa attgagcagg gtatcgatgg cactgacctg aaagccggaa ttatcgcgga
    33061 gatcggctcc agcgaaggga agattacgtc gctggaagag aaggtgttta ttgccgccgc
    33121 gctggcgcat aaccagaccg gacgcccgat ctccacgcat acgtcgttca gcacgatggg
    33181 gctggagcaa ctggctctgt tgcaagccca cggagttgat ctttcgcgcg tcaccgtcgg
    33241 ccactgcgat ctgaaagaca atctcgacaa cattttgaag atgatcgatc tcggcgcgta
    33301 tgtgcagttc gacaccatcg gcaagaacag ttactacccg gacgaaaagc gcattgcgat
    33361 gctccatgcg ctacgtgacc gtgggttgct gaaccgcgtc atgctgtcga tggatattac
    33421 gcggcgctct catttaaaag ccaacggtgg ttatggctat gactttttat tgaccacctt
    33481 tattccgcaa ttgcgccagt caggattcag tcaggccgat gtggatgtga tgttacgtga
    33541 aaatccctct caatttttcc aataaggaca gactcatgaa aaagattggc gttgcaggct
    33601 tacagcgtga gcagattaaa aaaaccattg aagcgacggc tcctggctgt tttgaagttt
    33661 tcattcacaa cgacatggaa gcggcaatga aagtgaaatc cggacaactg gattactaca
    33721 tcggcgcgtg taataccggt gcgggcgcag ccttgtcgat tgctatcgcg gtgattggct
    33781 ataacaaaag ttgcaccatt gccaagccgg gcattaaagc gaaagacgaa catatcgcca
    33841 aaatgatcgc tgaaggaaaa gtggcgtttg gcctttccgt tgagcacgtt gaacacgcga
    33901 ttccgatgct gattaaccat ctaaaataaa aggcacgact atggatctgt atattcagat
    33961 tatcgtggtg gcgtgcctga cgggtatgac atcgcttctg gcgcatcgct cggcggccgt
    34021 ttttcatgac ggtatccgcc cgatcctgcc acaactgatt gaaggctata tgaaccgtcg
    34081 cgaggcgggg agcatcgctt ttggtctgag cattggtttt gtggcctcgg tggggatctc
    34141 ttttaccctg aaaaccgggc tgctcaacgc atggttactc tttcttccta ccgatatcct
    34201 cggcgtactg gcgataaaca gcctgatggc gtttggtctt ggtgctatct ggggaatatt
    34261 gatcctcacc tgtctgttgc cagtaaacca gctgctgacc gcgctaccgg tggatgtatt
    34321 aggtagcctg ggggaattaa gctcgccggt ggtttcagct tttgcactgt tcccgttggt
    34381 ggcgattttc taccagtttg gctggaagca aagtctgatc gccgccgtgg tggtactgat
    34441 gactcgtgtg gtggtagtac gctatttccc acatcttaac cctgaatcca tcgaaatctt
    34501 tattggcatg gtgatgctgc tggggatcgc aataactcat gacctgcgtc atcgtgatga
    34561 aaatgatatc gatgccagcg gtatgtcggt gtttgaagag cgtacatcac ggattatcaa
    34621 aaacttaccg tatatcgcca tcgtgggtgc attgattgct gccgttgcca gcatgaagat
    34681 tttcgccggt agtgaagtgt cgatcttcac actggagaaa gcgtactccg caggcgtaac
    34741 gccggaacaa tcgcaaacac taatcaacca ggcggcactg gcagaattta tgcgcgggct
    34801 gggttttgtg ccgttgattg ccaccacggc gttagccacc ggcgtgtatg cagttgcggg
    34861 ctttaccttt gtttatgcgg tgggctatct ctcgccgaat ccgatggttg cagcggtatt
    34921 aggcgcagtg gttatttcgg cggaagtctt gctgcttcgt tctatcggca aatggttggg
    34981 gcgctacccg tcggtacgta atgcgtcgga taacatccgt aacgccatga atatgctgat
    35041 ggaagtggcg ttgctggtcg gttcgatctt tgcagcaatc aaaatggcgg gctataccgg
    35101 attctctatc gcggttgcca tttacttcct caacgaatcc ctgggccgtc cggtacagaa
    35161 aatggcggca ccggtcgtgg cagtaatgat caccggcatc ctgctgaatg ttctttactg
    35221 gcttggcctg ttcgttccgg cttaaggaag gcacctatga agacgtttcc tctgcaaagc
    35281 ctgacgctta ttgaggcgca gcaaaagcag tttgcgctgg tggacacgat ttgccgtcat
    35341 tttcctggca gcgagtttct tacctgcggt gatttgggct taacgccggg gctaaatcag
    35401 ccccgcatta cccagcgtgt ggaacaggtg ctggctgacg cgtttcacgc acaggccgcg
    35461 gcgctggtac agggcgcggg tactggcgcg atccgtgcgg cgctggcagc tttgctcaaa
    35521 tcggggcagc gtcttctggt gcatgacgcg ccggtttatc caacgacgcg cgtcattatt
    35581 gagcagatgg ggctaacgct cattacggct gatttcaatg atctgtcggc actgaagcag
    35641 gttgttgacg agcaacaacc tgatgccgcg ctggtgcagc atacgcgcca gcagccgcag
    35701 gaccgctatg tgctggcaga cgtgctggca acgttgcgcg cggcaggtgt tccagcgtta
    35761 accgatgaca actatgcggt gatgaaggta gcgcgaatcg gctgcgaatg cggggcgaat
    35821 gtgtcgacat tttcctgctt caagctattt gggccagagg gcgttggtgc ggtggtaggc
    35881 gatgccgatg ttattagccg gattcgcgcc acgctctatt ccggcggcag tcagattcag
    35941 ggggcgcagg cattagaggt gttgcgtggt ctggtgctcg cgccagtgat gcacgcggtg
    36001 caggcggggg tatctgaacg gttgctggct ttgcttaacg gcggcgcggt ggcggaagtg
    36061 aaaagcgccg tcattgccaa tgcacagtcg aaggtattga ttgtggagtt tcatcagccg
    36121 attgccgcca gagtgctgga agaggcgcag aaactcggtg ccttgcctta cccggttggt
    36181 gcagagtcga aatatgaaat cccgccgctc ttttatcgac tttccggaac gtttcgccag
    36241 gcgaatccgc agttagaaca ttgcgcgatt cgcattaacc caaatcgcgg cggtgaagag
    36301 acgatattgc ggatcttgcg tgaaagtatt gccagcattt aatcgttgta aacgatattt
    36361 tcgaaaagaa tgcagaaaca aatacgccct cggataactc cgagggcgtc aggcaattaa
    36421 accggcgtta gccgatttct gtcagagact tactgtgcag tcggaccgca accgccaatg
    36481 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    36541 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcgcctac cagtgcaact
    36601 ttaataaatt tgttcattta cattccctat gtattatata ttaattaata ttcattgatt
    36661 atatgaatat aagcagactg ctcattaaga acgatacagg aatattcaat tagcgtcagt
    36721 gagcgattgg cttaattttg taagttgctg taacggtgaa ta
//
LOCUS       DAJOTB010000032        34992 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJOTB010000032 DAJOTB010000000
VERSION     DAJOTB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.032"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(18..743)
                     /gene="phoU"
                     /locus_tag="OHN28_001612"
     CDS             complement(18..743)
                     /gene="phoU"
                     /locus_tag="OHN28_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCQ2297022.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(758..1531)
                     /gene="pstB"
                     /locus_tag="OHN28_001613"
     CDS             complement(758..1531)
                     /gene="pstB"
                     /locus_tag="OHN28_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCQ2297023.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="OHN28_001614"
     CDS             complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="OHN28_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCQ2297024.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="OHN28_001615"
     CDS             complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="OHN28_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCQ2297025.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="OHN28_001616"
     CDS             complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="OHN28_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCQ2297026.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="OHN28_001617"
     CDS             complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="OHN28_001617"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCQ2297027.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="OHN28_001618"
     CDS             complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="OHN28_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCQ2297028.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8626..9045)
                     /gene="atpC"
                     /locus_tag="OHN28_001619"
     CDS             complement(8626..9045)
                     /gene="atpC"
                     /locus_tag="OHN28_001619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCQ2297029.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="OHN28_001620"
     CDS             complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="OHN28_001620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCQ2297030.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="OHN28_001621"
     CDS             complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="OHN28_001621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCQ2297031.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="OHN28_001622"
     CDS             complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="OHN28_001622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCQ2297032.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="OHN28_001623"
     CDS             complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="OHN28_001623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCQ2297033.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="OHN28_001624"
     CDS             complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="OHN28_001624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCQ2297034.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="OHN28_001625"
     CDS             complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="OHN28_001625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCQ2297035.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="OHN28_001626"
     CDS             complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="OHN28_001626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCQ2297036.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="OHN28_001627"
     CDS             complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="OHN28_001627"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCQ2297037.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16130..16753)
                     /gene="rsmG"
                     /locus_tag="OHN28_001628"
     CDS             complement(16130..16753)
                     /gene="rsmG"
                     /locus_tag="OHN28_001628"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCQ2297038.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16817..18706)
                     /gene="mnmG"
                     /locus_tag="OHN28_001629"
     CDS             complement(16817..18706)
                     /gene="mnmG"
                     /locus_tag="OHN28_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCQ2297039.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19085..19528)
                     /gene="mioC"
                     /locus_tag="OHN28_001630"
     CDS             complement(19085..19528)
                     /gene="mioC"
                     /locus_tag="OHN28_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCQ2297040.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHNIPEDPAEEWLGSWINLLK"
     gene            complement(19618..20076)
                     /gene="asnC"
                     /locus_tag="OHN28_001631"
     CDS             complement(19618..20076)
                     /gene="asnC"
                     /locus_tag="OHN28_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCQ2297041.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20228..21240
                     /gene="asnA"
                     /locus_tag="OHN28_001632"
                     /pseudo
     CDS             20228..21240
                     /gene="asnA"
                     /locus_tag="OHN28_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
     gene            complement(21245..22696)
                     /gene="viaA"
                     /locus_tag="OHN28_001633"
     CDS             complement(21245..22696)
                     /gene="viaA"
                     /locus_tag="OHN28_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCQ2297042.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22690..24186)
                     /gene="ravA"
                     /locus_tag="OHN28_001634"
     CDS             complement(22690..24186)
                     /gene="ravA"
                     /locus_tag="OHN28_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCQ2297043.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            24409..26277
                     /gene="kup"
                     /locus_tag="OHN28_001635"
     CDS             24409..26277
                     /gene="kup"
                     /locus_tag="OHN28_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCQ2297044.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            26444..26863
                     /gene="rbsD"
                     /locus_tag="OHN28_001636"
     CDS             26444..26863
                     /gene="rbsD"
                     /locus_tag="OHN28_001636"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCQ2297045.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            26871..28376
                     /gene="rbsA"
                     /locus_tag="OHN28_001637"
     CDS             26871..28376
                     /gene="rbsA"
                     /locus_tag="OHN28_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCQ2297046.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            28381..29346
                     /gene="rbsC"
                     /locus_tag="OHN28_001638"
     CDS             28381..29346
                     /gene="rbsC"
                     /locus_tag="OHN28_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCQ2297047.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            29371..30261
                     /gene="rbsB"
                     /locus_tag="OHN28_001639"
     CDS             29371..30261
                     /gene="rbsB"
                     /locus_tag="OHN28_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCQ2297048.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            30387..31316
                     /gene="rbsK"
                     /locus_tag="OHN28_001640"
     CDS             30387..31316
                     /gene="rbsK"
                     /locus_tag="OHN28_001640"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCQ2297049.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            31320..32312
                     /gene="rbsR"
                     /locus_tag="OHN28_001641"
     CDS             31320..32312
                     /gene="rbsR"
                     /locus_tag="OHN28_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCQ2297050.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(32278..33705)
                     /gene="mdtD"
                     /locus_tag="OHN28_001642"
     CDS             complement(32278..33705)
                     /gene="mdtD"
                     /locus_tag="OHN28_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCQ2297051.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(33728..34420)
                     /locus_tag="OHN28_001643"
     CDS             complement(33728..34420)
                     /locus_tag="OHN28_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCQ2297052.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            34901..>34992
                     /locus_tag="OHN28_001644"
     rRNA            34901..>34992
                     /locus_tag="OHN28_001644"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         8465 a         9034 c         8922 g         8571 t
ORIGIN      
        1 gggatttact tggtgaatta tttatcgcta tctttttctg ccagcagttt atcgagctcg
       61 tcaccaccga cgtgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata
      121 ttctggcagc ggtcgccaat acgttcgata gaacgcgcac agaataacgc ggtaagtacg
      181 ctcggaatgg tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
      241 tactcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
      301 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
      361 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
      421 ttatccgcca cgtcaccaat acgctccagc tcggcaatgg ttttactgat taccatcacc
      481 agacgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
      541 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tgacgcgctt cgccagatcg
      601 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
      661 atggtcatca cctgcgtgcg gatgctttcc agttcggcgt tgaactggcc ggaaatatgt
      721 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
      781 agtcttctgt ttgttttttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
      841 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
      901 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
      961 gcccggtaga gatagggtcg agtgccgaac acggttcgtc gagcagcagc acttccgggc
     1021 gaatggcgat accacgcgca atgcacagac gctgttgctg accaccagag agagagtaac
     1081 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
     1141 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgcacg ccaaaggcga
     1201 tgttgtcgta aatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
     1261 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatcg agcagaattt
     1321 caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
     1381 ttgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg gcgatatcca
     1441 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
     1501 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tgcctgatgc
     1561 acttcgcgaa tcaggcttac cgtaggccgg ataaggcgtt tacgccgcat ccagcaaagt
     1621 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcagtacgc
     1681 acagggtaat gatcaacacc ccggcccagg ccagttgctg ccattccgca aacgggctca
     1741 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
     1801 agaactggtt ggagagcgca gtaaacagca gcggtgcggt ttcaccagca atacgggcaa
     1861 tcgccagcag gataccggtc atgatcccgg acaccgacgc tttcagcgtg atcgaggcga
     1921 tcatcttcca cttcggcgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
     1981 gtttcagcat gttctcggtg gtgcggataa caatcggcac ctgcaacagc gccagcgcaa
     2041 tcacgcccgc ccagccggag aagtgctcca tctgcgccac cacgatggtg taaacaaaaa
     2101 gaccaaccac aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg
     2161 ccagccagga tttacgacca tattcagcca ggtaaatccc cgccataatg cccagcggcg
     2221 taccgaatac cgtagcccac aaaattaaca ggccgctacc cgccagggcg ttcgccagac
     2281 caccgccttc cgtattgggt ggtggcgtca tttcggtgaa cagcgccagc gacataccgt
     2341 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacaga ccgaaggcca
     2401 tcgtcgccat tgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt
     2461 tgcggcggga ttcagccagt gccgcagtgg tttgcatttc aaccatagcc attagcgtgc
     2521 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
     2581 aatcacaaac aggatcaggc ccagttccat cagtgcagca acgtgcagac ccgactctgc
     2641 ttcggcaaat tcgttcgcca gcgcagaggt gatactgttg cccggcatgt acagcgaggc
     2701 gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccaagcgc
     2761 gcggcccagc cccaacataa tgccgccgat aacaccattt ttggtgaacg gaagaacgat
     2821 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
     2881 cggggtttgt tcgaacacat cgcgcattac cgccgcaatg tacggaataa tcatgatggc
     2941 gaggatcacg cctgccgcga ggataccgat accaaaggcc gggccggaga acagcgcgcc
     3001 gacaatcggg atattcgaca tgatattgcc gaccggctcc tggaagtaaa cggcgaacag
     3061 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag
     3121 ctcaatggcg atacccagcg ggcgcttcag ccagccaggc gcaagctcgg tcaggaacag
     3181 ggcgataccg aaactcaccg ggacggcgat cagcagcgcg ataaacgagg tcaccagcgt
     3241 accgtagatc ggcaccagcg ccccgtagat atcgttcggc gcatcccact ctttggtcca
     3301 caggaaagcc agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat
     3361 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
     3421 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
     3481 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta
     3541 cccggcctga gttttattag tacagcggct tgccgctact gtctttaata ttggtcttcc
     3601 acgcagcgcg aacctgttcc actacgctat ccggcaggct ggcgtaatcc aggtcgttcg
     3661 cctgtttagc cccggttttg tatgcccagt cgaagaactt cagcacttct gtgccttgtt
     3721 ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata ggccacgcat
     3781 cttcgccttt ctggttggtc agatcctgag cgaatgtttt gctccagtct gcgccttttg
     3841 ctgcattagc gaagttttct tcggtcggac taaccggttt gccatcagcg gagatcaatt
     3901 tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacgtaacca attgcacccg
     3961 gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga ccgatcggcc
     4021 attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccaggt
     4081 agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca gcaatgttct
     4141 gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc cacttcttga
     4201 ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca
     4261 gacctggaat attaaccgcc agcaccacgc cgccaatcac ggtcgggaac tggaacaggc
     4321 cttcctgagc cagtttttca tcagacagcg gcgcgtcaga ggcaccgaaa tcaacggtgt
     4381 tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac
     4441 cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac
     4501 ctgcacctgt caggcttgct tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg
     4561 cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata
     4621 aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatgtaa
     4681 ggtttatgac agttttatga cagagagaga aagtctttag tctgatttaa ataagcgttg
     4741 atattcagtt aattacaaat attaataacg aagagatgac agaaaaattt tcattctgtg
     4801 tcagagaaaa agtagccgaa gatgacggtt tgtcacatgg agttggcagg atatttgatt
     4861 aaaaacataa caggaagaaa aatgccctgc ttacgcaggg catccattta ttactcaacc
     4921 gtaaccgatt tcgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg
     4981 tggtaagcca gtagttgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
     5041 tgcggcatct caatgatgtg catgttgtca ctgctcacaa aacccgcatc ctgatcggcg
     5101 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
     5161 agcaattcgt tgttcggtgc aacgacgata accggcatat cggcatcaat cagcgccagc
     5221 ggaccgtgtt tcagttcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
     5281 agcttcaatg caccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
     5341 tgatgtttgt cagagaaatc ttctgccagc gcttcaatgc gtttgtcctg agacagcatc
     5401 tgctcaatac ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca
     5461 tccagacctt tcaggcgaga cagtttcgcc accagcatca acagcacagt taactgggtg
     5521 gtgaatgctt tggtggaggc cacgccaatt tccgtaccgg cgttggtcat cagcgccaga
     5581 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
     5641 cccagctctt tcgaaagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
     5701 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
     5761 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
     5821 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttca
     5881 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     5941 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg gcggtaaata
     6001 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     6061 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
     6121 aggaagataa aacgacgagt cactggcaac agcgccagct ggtcagaagc gataaagttt
     6181 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
     6241 ggatgacggg agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc
     6301 agaactgcct cacgcagagt accgccttgt ttcagctccc agttcaccaa atgtgcaatc
     6361 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
     6421 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
     6481 tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg
     6541 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctgtacttt accgaggcga
     6601 cgcaggcggg tcatatgacc ttcagcatcc accacggcca ggccggcaga gtcatatccg
     6661 cgatattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
     6721 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     6781 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     6841 gcttgtgggc gggagattat gttatctcct catcccatat gaccggatta cccgaccaga
     6901 attacttttt ctttatcgga cgacgccagc cttctttttg agtctgcggc acacggctga
     6961 tagccagggc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
     7021 ctttgcctac tgttaccggg gccaccagtt gagtgtcgga accgacaaac acatcgtcgc
     7081 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcaccga
     7141 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
     7201 ctttacccag acgcgctttt ttcatctcaa cgaagttacc gacgtgtgca ccttccagca
     7261 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg
     7321 catcttccac gacggtatat ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
     7381 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     7441 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg
     7501 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     7561 cgcgctccag tcgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt
     7621 gcggatgaac ggcgacgatt tcacgtcctt cctgatacgc cagcgcaata atgtcggtga
     7681 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     7741 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     7801 catctttgtg ctcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac
     7861 cggtcggatc atccagtttt accgtcagca gaccaatgcc accctgcggt ttagcatcac
     7921 gcagacgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     7981 cttcatcatc ggcaaagaaa ggtgcggcct gctgcatcgc atgacccgta cccagctgtt
     8041 ctgcctgaag cacccagttc aggttgtcgt ctttcagcgc ctgcttaagc agatcgccgc
     8101 cgtgaccata cacaaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     8161 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     8221 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     8281 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     8341 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc
     8401 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     8461 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     8521 ttttatatca tcaggatcta tgtgaacgct tttcaggacg ggtcaatacg cgtaaaaaaa
     8581 agccagcctg ttgccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt
     8641 ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc
     8701 gtaatctacg tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat
     8761 ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt
     8821 gccaggctgc acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg
     8881 tttcacgatg cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta
     8941 gatccccagt tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat
     9001 ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta
     9061 aggcgttaaa gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac
     9121 gcctgctccg gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg
     9181 tctttcaggg agacgtattt acccggagaa ccggtgaata cctctgccac gaagaacggc
     9241 tgggacagga agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac
     9301 agttcatcca tacccaggat ggcgataatg tctttcagtt cctgataacg ttgcaggatg
     9361 gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga
     9421 cggctggtgg agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg
     9481 ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcagg
     9541 tcatccgcag gtacgtatac cgcctgtacg gaggtgatag aaccggtttt ggtggaggtg
     9601 atacgttcct gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa
     9661 ggcatacggc ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg
     9721 tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg
     9781 gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat
     9841 actttgtcga taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta
     9901 cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaata
     9961 agctccatca tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg
    10021 cccttagcga acggacacat caggtcgata actttgatac cggtttccag cagttcctga
    10081 gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca
    10141 ccgatctcgc ctttcatgtc gaccggttca cccagtacgt tcatgatacg acccagagtc
    10201 gctttaccta ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc
    10261 agaccgtcgg aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga
    10321 acttccagca ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt
    10381 acggcatcct gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca
    10441 gtagccatct taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga
    10501 tctcggtgag ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct
    10561 ctttaatcag gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct
    10621 gctcgctggc caggttttca accacgccct gataaacctg agattcgaca taacgacgca
    10681 gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt
    10741 tcagatcatc atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag
    10801 acatggtgtt aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt
    10861 aggcctgcaa catcactttt accggaccga tcagttcgga caaggaaggg ttatccccca
    10921 tgccggtgac ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg
    10981 agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca
    11041 gcagtttttt gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca
    11101 ccaggtagcc cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat
    11161 taccgtgtgc aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg
    11221 ccatgcgatc ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga
    11281 tcttttgcgt gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga
    11341 gcttctcctc aatgccttgc ggcctgccca aaggcaagcc gccagacgtt accaggattg
    11401 ggttgctttg aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc
    11461 accggtctgg ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccaacag
    11521 agcggcttcg aagctgccga ttttcgacag ttcaacatcc gccaggtaac cacgttctgc
    11581 tgcgaacaga accagagact gttgcgcaac agacatcggc gcatactgtt tctgcttcag
    11641 cagttcggtc actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga
    11701 tgcaaactga gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc
    11761 ggacagtttt ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc
    11821 cgggttaacc gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc
    11881 atcggtaatg gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc
    11941 gataatcggc agcgcagtca gagaaccggt tttccctttc acttcacctt tggtgaaagc
    12001 ttcaacgtat tcggcgttaa cacgcgcagc acgctccagc agacgagagt ggaggtagaa
    12061 tacgtcgccc gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg
    12121 gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc
    12181 acggaagtat tcacccattg cgcaaccagc gtacggtgcc aggtattgca gtgcagcgga
    12241 ttcagacgcg gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt
    12301 acgtaccacg ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat
    12361 accggaatcg cgctggttga tgatggcatc gatagccagt gcggttttac cggtctgacg
    12421 gtcaccgatg atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt
    12481 ataaccggtc tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc
    12541 ttctacagca gagaagccgt cgtgatccag cggaccttta ccatcgattg gtgcacccag
    12601 agtgttaacc acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt
    12661 acacttaact ttcatgcctt cggcaaggtc agcgtacggc cccataacaa ccgcacctac
    12721 agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc
    12781 accctgcata caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat
    12841 agtaccttcg ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag
    12901 ttcgctgatt tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc
    12961 tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat
    13021 gataacacct gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag
    13081 acgtttttcc atcgcagcag aaattttcgc gagctgttgt tcactcaacg cggcagcgga
    13141 aatgacgtct acctcagcgg tagcctcact cacagcacgc aggtgaataa actgctccag
    13201 aacatccggg agcgcgttaa gacgaccatt ttcggccatt acccgaatca ggttctgacc
    13261 gttttcgtcc agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc
    13321 aagcgcgcca gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc
    13381 cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt
    13441 ggcgtagggg cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca
    13501 gcgacaagtt tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc
    13561 ttctcggcgc cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt
    13621 ttacgctcgg cttcaatttc cgcctgcgcc tgggccacga ttttagtacg ttcctgttct
    13681 gcctcagctt tcgcttcatc cagaatctgc gagcggcgtt tgttagcctg ctcgatgatt
    13741 acctgggctt ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca
    13801 aggtccttat gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt
    13861 tcgatggctg ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca
    13921 aacgcgatgg cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta
    13981 gttaacgttc tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat
    14041 cacgtacaga cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat
    14101 aaagaactga gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa
    14161 tttacccccg aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac
    14221 agcggcagcc atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt
    14281 tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga
    14341 cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg
    14401 cccacggcac attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga
    14461 aaatcagctc accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg
    14521 acagcaggct taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg
    14581 gctgcagcgt caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca
    14641 gaatcaggat aaatacgccc agtgccatag acagcgttac gttcacgtca gcagacggaa
    14701 ccacacgcag tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag
    14761 gcagtaaatc catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag
    14821 caatcagctt gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca
    14881 ccagctcaat cgcggtctga aacttacctg gcacgccgct ggttgccttt ttggctacac
    14941 tacggaataa aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat
    15001 tgattgtcca gaaggtggct ggggggttat gtggatccac cagcgagaat gtacgcaggt
    15061 ccagctgaag gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag
    15121 ccatgatgcc ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag
    15181 caccaaaacc cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac
    15241 caccagtaac accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc
    15301 cacccggcct ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac
    15361 gttaggcaga aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt
    15421 gaggctgaac agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg
    15481 agcaacgttt cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga
    15541 ggtatgccgc gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg
    15601 tcaaattata cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat
    15661 gtttaaatat ttttccagtg catacaattg cgacttttct gcaaaccctg ttcgatcatg
    15721 aaaaactgta aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg
    15781 catgttcgcg cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata
    15841 tttcagtcta aaaacagata ctgttttaat aaatgacatt tacataacaa aaaccagcca
    15901 ttgacatttt taataatgtt ttaacagtca atgatgattc ttagcgccga tttttagcag
    15961 actgatattt tcacttatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt
    16021 gatacaaaag agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt
    16081 gttgttaaca gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat
    16141 ttgctttaat caccaccaga tgacgttcgc catccagggc tggaacctga agtttaacca
    16201 ctgattcgac ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt
    16261 tcagcgcgta gaaacggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt
    16321 tcagagaggc aaaagcgcgg ctaattacgc catcaaatgg cggctctgac ggaaactctt
    16381 ctaccctgct ctgtactggc tcaatattct ccagtttaag ctcatgttgc acctgacgaa
    16441 ggaaacgcac gcgtttacca aggctatcca acaacgtgaa atgagcttca ggacgcacga
    16501 tagagagtgg aatgccaggc agtcccggtc cggtgccgac atcgataaac cgttcacctt
    16561 gcagatacgg tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat
    16621 cgcggaccga agtcaggttg tacgctttat tccatttatg cagcatattc acgtaggcaa
    16681 taagctggtt tttctggtga tcggtaagcg aaatacctgc gtctttcagc agtaaggaga
    16741 gtttgttgag cacggtgatt acctgttctt gatgcgttgc ctggtaagcg ggtgcttacc
    16801 aggcattttt aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca
    16861 gcagaatgga gatagccgca ggcgtgacgc cagaaatacg cgaagcctgg ccaatagagg
    16921 ccggtttgtg atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt
    16981 aatccagcgt cgctggtagc agggtattct cgttacgcag ctgcttttcg atctcatctt
    17041 gctggcgcgc gatataacct tcgtatttaa cctgaatctc aacctgttcc gcagcctgtt
    17101 catctgtcaa cgcaggggca aacggtgtca gcgtggttaa tttttcataa gtcatttcag
    17161 gacgacgtag cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat
    17221 tcacttcggc tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct
    17281 cacgctcgat attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc
    17341 ccagttcacg accgatttca gtcaaacgca gatccgcatt atcttcgcgc agcatcagac
    17401 gatattctgc gcgcgaggta aacatacgat acggttcttt ggttcctaaa gtgcacaggt
    17461 catcaaccag tacgccgaga tacgcctgag aacgcgccgg agcccaacct tctttgtcag
    17521 cagacagacg ggcagcgtta agaccagcca gcaaaccttg cgcagcggct tcttcgtaac
    17581 cggtagtgcc gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg
    17641 tcggtttcag gtcgcgtgga tcgaagaagt catactcaat ggcataaccc ggacgcacga
    17701 tcttcgcgtt ttccatcccc tgcatggagc ggacgatttg catctgcaca tcgaacggca
    17761 ggctggtgga gataccgttc ggataaattt cgttagaggt cagcccttcc ggttcaagga
    17821 agatctgatg ctgatttctg tcggcaaagc gcatgacttt gtcttcaatc gacgggcagt
    17881 agcgtgggcc gacaccttcg atcacccctg cgtacattgg gctacgatcg agattactgc
    17941 ggatcacatc atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat
    18001 gctgggacgc attgcccata aacgagaata ccggcattgg gttatcgcca tgctgttgcg
    18061 ccagtacgct aaagtcgatg gttcgagcat caatacgcgg tggtgtcccg gttttcagac
    18121 gaccaacgcg cagcggcagt tcacgcaaac ggcgagaaag cggaatggac ggcggatcac
    18181 cagcacggcc accgctgtaa ttatccagac cgatatgaat tttaccgtcg aggaacgtcc
    18241 caacggtgag cacgacagct ttggcacgga acttcagtcc catttgggta acggcaccga
    18301 ccacgcgatc gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg
    18361 gttggttctc cagcgccgta cgtaccgcct gacggtagag cacacgatct gcctgagctc
    18421 gggtagcgcg aaccgccggt cccttgcttg cgtttagtat cctaaactgg atacccgcct
    18481 gatcgatcgc tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct
    18541 tcccaatacc gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg
    18601 tcaaaagcag agtctgttga cccatacgcg ccgcggccat cgcggcctcg gtgcctgcat
    18661 gacccccgcc aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc
    18721 ataacgcggt atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc
    18781 ttgagaaaga cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt
    18841 ctattgtgat ctcttattag gatcgcactg ccctgtggat aacaaggatc cggcttttaa
    18901 gatcaacaac ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag
    18961 ctgggatcag aatgaggggt tatacacaac tcaaaaactg aacaatcgtt gttctttgga
    19021 taactaccgt ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt
    19081 atacttattt gagtaaatta atccacgatc ccagccattc ttctgccgga tcctccggaa
    19141 tgttgtgatc aagaatgttg atcttcagtg tttcgcctgt ctgttttgct ccggaatttt
    19201 tgagttctgc ctcgatttta tcgatagccc cacaaaaggt gtcatattca cgactaccaa
    19261 taccgattgc gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat
    19321 agaaaggaga aaggttgtcc ggaatatctc cggcaccgtg ggttgagctg ataaccagcc
    19381 agatccccga ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac
    19441 ccgcctcttc cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc
    19501 tgccgctgat aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg
    19561 tacgctgtga acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatca
    19621 gggcttaatg gtacgcatga tcgggttctg caggacgatc agtgtctcgg tggactgaat
    19681 ttcatcaatt gtttggatct tgttgataag tacatgctgg agagcatcga tcgaacggca
    19741 catcactttt ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc
    19801 gaggctttcc agctttgcca gcgcggaagg atagtctttg gcgctcttta atataatgcc
    19861 gataaagcag cctacgtcat aaccaagctg cttcgggctg acatcaatac gcgccccggt
    19921 aatgatcccc gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc
    19981 aaattgtttc gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat
    20041 gccacggtcc agattgtcga tcagataatt ttccatagga ttttcttatg cggattgatg
    20101 attcattcta ttttagcctt cttttttaat gaatcaaaag cacgtgaggc tttttattga
    20161 atgattattg catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt
    20221 ataaaaaatg aaaaccgctt acattgccaa acaacgtcaa attagcttcg tgaaatctca
    20281 cttttctcgt caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag
    20341 ccgtgtgggg gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa
    20401 agtgaaagct ctgcctgatg cccagttcga agtggttcat tcactggcga agtggttcat
    20461 tcactggcga agtggaaacg tcagacctta gggcaacacg acttcagcgc gggcgaaggg
    20521 ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc gtctttcccc gttgcactcg
    20581 gtctatgtag accagtggga ctgggaacgc gtaatgggcg acggcgagcg tcaattctcg
    20641 actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga agctgcggtt
    20701 agcaaagagt ttggcctggc accgttcctg ccggatcaga tccacttcgt acacagccag
    20761 gagttactgt ctcgttatcc ggatctcgat gccaaagggc gtgagcgggc gattgcgaaa
    20821 gatcttggcg cggtattcct cgtcgggatt ggcggcaagt tgagtgatgg tcatcgtcac
    20881 gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg ccatgcgggt
    20941 ctgaacggcg atattctggt gtggaacccg gtactggaag atgcgtttga gctttcttcc
    21001 atggggatcc gcgtggatgc cgacacgctg aagcatcagc tggcgctgac cggtgacgaa
    21061 gatcgcctgg agctggagtg gcatcaggcg ctgctgcgcg gtgaaatgcc gcagaccatc
    21121 ggcggcggta tcgggcagtc tcgtttgacc atgctgctgc tgcaactgcc acatatcggc
    21181 caggttcagt gtggtgtatg gccggcagct gttcgcgaga gcgtcccttc tctgctgtaa
    21241 taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat cccggtatca aagcgccaga
    21301 tatgatcgaa aatgcgcatg atgccgggtt tgccatgtgc agacatcgcc acggcatgaa
    21361 agcgatgctg atgtacccgc tgtagctctt tcactttact cgtcacgtcg tcaggcaacc
    21421 gctgggcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc cggctttgca
    21481 agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga aactgctggc
    21541 ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa cggacgatct
    21601 cggtggaaaa tagcataata tagcagcgcc ggttttcagc gagagcaatg cgcatcaagg
    21661 ccaggcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg gaagtatcca
    21721 cacagacaat aaacggtccg cgcggctgtt cgtcgtaatc tttatgcacc accgggcgtt
    21781 cgatcacttt ttcacgccac gactcaccat gcaggcgata ggtgagcaac tgtttttcca
    21841 ccagccgacg gtaaaactca tactccagtt ctgttatccc tagtgtcgcc agttctggcg
    21901 gcaggagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca ggaaccgtcg
    21961 ccggttcgcg caccagggtg cggaaggttt ccatctgcgc atcgttgcgc ggtattgatt
    22021 tggcttcccg ggaacgcccc agctgttctg ccaggcgttt cagttccggc tgttcgttaa
    22081 gaaattcacc gtatttcaca atcaactgat agtcgccacg tttaagctga ccggcgctca
    22141 tatcccacag acgaccagct gcggtattgt tatctgcgag aatcggttca agttgtccgc
    22201 tcagcgtcat gcgttcctga acttcactca acagttgttc gcgttcttct tctaatagct
    22261 gttgatttaa cgtcgttgct tgcacgatca gacttaaacg ccaacgctgg agaaaaagcg
    22321 tgtgtaacgc tgaagtgatc gtgctgttag catcaaccaa ctgtcgggct tgttctgccc
    22381 atggggaatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca ataaactgcg
    22441 gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt tccggcggga
    22501 ctcgggcatc tttcagccga ctgcgcagcg cctcacgcca gcggggaaca tcatcagtga
    22561 ttgccgcctt cagtcgtggg aatttttcaa agaagactgc cagctgcggt gaggccagca
    22621 gcgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc acattaagcg
    22681 tatccagcgt tagcattgtt gtgcctggcg aatctgtgcg ccgacatcct gtaggctggc
    22741 ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt cgctgaataa
    22801 agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt tgttgtttta tttcacctgg
    22861 cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac ttacatcgcg
    22921 tacaacaaga tgttgggcgc tatccacttc cagattcagt ttctgggcaa agccgatacc
    22981 gttcagtttg ccgcgaatct caccaccttt actcagccac tgttccagcg cgctacgctc
    23041 aaaggagata tgaaccactt ccatatcatg caattttaac ggtttttgca gcagcagagt
    23101 cagagtggaa gcagtaacgt taacaggaag ttgatactgc tgacgacggc tgaaaatgcc
    23161 gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgga gttgcaggtg
    23221 acgttgcaca atcgcgccca ggcgggtcaa catcccttgc tgttgccagg catgaccggt
    23281 catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat accacaggca
    23341 atctttcagc aaaatgagat caaccggggc aacagcactg cgaccgctaa aaaaggcgct
    23401 ggcctgcaat aagcggatcg cttttttcca gcgacgatcc gagacataag gcgcatccgg
    23461 taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat cgggcagcgt
    23521 aatttcacca atctctttct gccagcgttc atattcttca tctgtgatct gcaaggaggc
    23581 aggaaccgga ttgtcatttt catcctgttg actggtcagc atggagcgga aattcgcttt
    23641 atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg cttccagact
    23701 gctgtctgct tccggcagct cgttggaggc cgccaccagc agacgcatcg ggattttttc
    23761 tacgagtgcg ccgttgcgga actggcgctc gttaatggcg gtgagcaagg tattaagaat
    23821 tgccggaccc gctttccaga tctcatccag aaagacgatt tcagcttccg gcaggtaacc
    23881 gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa ggggaccaaa
    23941 aacttcttcc ggcgtggaga agcgggtcat cagatattca aacgcgcggg cattctgaaa
    24001 ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggtgggc caaggaggaa
    24061 cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc tacgttcata
    24121 aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata aatgagggtg
    24181 agccataatg aagtggcgtc ctttcatcaa aagttctgcg taaattgcga gtatagacgt
    24241 ttcttgctgg tggctaaaat agtctcaaag gaggggtatt tttctttgag ccaggttaat
    24301 gtggccgcat ttaggagtac gattttgccg ttaatcgtgc atactgtgcg cttttttgtg
    24361 ggccaaggga ctaagcacac atttcatatt tcaacgaaag actagtctat gagcactgat
    24421 aataagcaat cattgcccgc gattaccctc gcggcgattg gagttgtcta cggcgatatt
    24481 ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg gccagtttgg ttttggcgtt
    24541 gaacgcgatg ccgtgtttgg ctttttatcg ctgatcttct ggctgctaat ctttgtggtt
    24601 tccattaaat atctcacctt cgtgatgcgg gcagataacg ccggtgaagg ggggatcctg
    24661 acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa ccacatcaat gctggtgatt
    24721 atggggctaa tcggcggcag ctttttctat ggtgaagtcg tcattactcc cgctatttcg
    24781 gtgatgtccg ccattgaagg tctggaaatc gtcgccccgc agctggatac ctggatagtt
    24841 cccctctcaa ttatcgttct cacattatta ttcatgattc aaaaacatgg cacagctatg
    24901 gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt tgattctggc agggctgggg
    24961 ttacgtagca ttattgctaa cccggaagtg ctgcatgcac tgaatccgat gtgggcggtg
    25021 catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt ggtgctgtcg
    25081 attacggggg tcgaggcgct gtatgctgat atggggcact ttggtaagtt ccctattcgt
    25141 ctggcgtggt tcaccgtcgt attgccttcc ttaaccctta attacttcgg ccagggagcg
    25201 ctgttgttaa agaatccgga agcgattaag aacccgttct tcctgttggc accggactgg
    25261 gcgctgatcc cgctgctgat catcgccgca ctggcgacgg taattgcctc gcaggcggtt
    25321 atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg
    25381 cgcattattc acacctccga aatggagtca gggcaaatct atattccctt tgtgaactgg
    25441 atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag caacctggcg
    25501 gcggcgtacg ggattgcggt gaccggaacc atggtgctga cgtctattct ctcgactacc
    25561 gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct gattgctttc
    25621 ctttgtgtcg atattccctt gttcaccgct aacctcgata aactgctctc cggtggctgg
    25681 ttgccattga gcctcggtac ggtgatgttt atcgtgatga ccacctggaa gagcgagcgt
    25741 ttccgcttgc tgcggcggat gcatgaacat ggtaactctc tggaagcgat gattgcttcg
    25801 ctggagaaat caccgcccgt tcgcgtgcct gggaccgcgg tgtatatgtc gcgtgcaatc
    25861 aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt gcatgagcgg
    25921 gtgattctgt taactctgcg caccgaagac gcgccatatg tccataacgt ccgtcgggta
    25981 cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg ttggcgagaa
    26041 acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag ctgccggatg
    26101 atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat
    26161 ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg ggcaccggat
    26221 caatttgaaa tcccgccaaa cagagttatc gaactgggta ctcaggtcga aatctaacgc
    26281 cagacgcctc ctttctccat aagggggcgt ttttgttttc atggttaatc accatgtaaa
    26341 acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac tcagtcagga
    26401 ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt
    26461 tcttaattct gatatttcat cggtgatctc ccgactggga cataccgata cgctggtggt
    26521 gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt atcgatatgg cattaaccca
    26581 gggtgtacct tcttttatgc aggtgctggg cgtcgtcaca aatgaaatgc aggtcgaggc
    26641 tgtcattatc gcggaagaga tcaaacaaca taatccgcaa ctccacgaaa cgttgctcac
    26701 tcaccttgag cagctgcaac aacaccaggg aaacaccatt gaaattcgtt acaccacgca
    26761 tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg gagaatgttc
    26821 tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc atggaagcat
    26881 tacttcagct taaaggcatc gataaagcct ttccgggcgt aaaagccctc tcgggcgcag
    26941 cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaaacggc gcgggtaaat
    27001 ccaccatgat gaaagtgctt actggtatct atactcgcga tgccggtacg cttttatggt
    27061 tggggaaaga aacgacattt accgggccga aatcttccca ggaagccggg attgggatta
    27121 tccaccagga actgaacctg atcccgcagt taaccattgc cgaaaacatt ttcctcggtc
    27181 gtgagtttgt taatcgcttt ggcaaaattg actggaaaac catgtatgcc gaagcggata
    27241 aattgctggc taaacttaac ctgcgcttta aaagcgacaa actggtgggt gatctttcca
    27301 tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag ctttgagtcg aaagtcatca
    27361 ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccttg ttccgcgtca
    27421 tccgcgagct gaaattgcaa ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa
    27481 tcttcgagat ttgcgatgac gttaccgttt ttcgtgacgg acaatttatt gctgagcgtg
    27541 aagtggcatc actgaccgaa gattctctga ttgagatgat ggtgggccgc aagctggaag
    27601 atcaatatcc gcacctggac aaagcgccgg gagatatccg cctgaaagtc gataatctct
    27661 gcggacctgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt cttggcgtct
    27721 ctggtttgat gggcgctggg cgtaccgaac tgatgaaagt gctctacggc gcactgccgc
    27781 gcacaagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttcg ccgcaggatg
    27841 gcctggcaaa cggcattgtt tatatctccg aagaccgtaa acgtgacggt ttagtgctgg
    27901 gcatgtcagt aaaagagaac atgtcgctga ccgcgctgcg ctacttcagc cgcgctggcg
    27961 gcagtttgaa gcatgccgat gaacagcagg ctgtgagtga tttcattcgt ctgtttaatg
    28021 tgaaaacgcc atcgatggaa caggcaattg gtctgctttc cggtggcaat cagcaaaaag
    28081 tagcgattgc ccgtggtctg atgacacgcc ctaaagtgtt gatcctcgat gagcctaccc
    28141 gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact gattaaccag ttcaaagccg
    28201 atggcttgag catcattctg gtgtcatcgg agatgccaga agtattaggc atgagcgatc
    28261 gcatcatcgt catgcatgaa gggcatctca gcggggaatt tactcgtgag caggccaccc
    28321 aggaagtgtt aatggctgcc gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa
    28381 atgacaaccc agactgtctc tggtcgccgt tatttcacga aagcgtggct gatggagcag
    28441 aaatcgctta tcgctctgct ggtgctgatc gcgattgtct cgacgttaag cccgaacttt
    28501 ttcaccatca ataacttatt caatattctt cagcaaacct cggtgaacgc cattatggcg
    28561 gtcgggatga cgctggtgat cctgacgtcg ggtatcgact tgtcggtagg ttctctgttg
    28621 gcgctgaccg gcgcagttgc tgcatctatc gtcggcattg aagtcaatgc gctggtggct
    28681 gtcgctgccg ctctcgcgtt aggtgccgca attggtgcgg taactggggt gattgtagcg
    28741 aaaggtcgcg tccaggcgtt tatcgctacg ctggttatga tgcttttact gcgcggcgtg
    28801 accatggttt ataccaatgg tagcccagtg aataccggct ttactgagaa cgccgatctg
    28861 tttggctggt ttggtattgg tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg
    28921 attgtcttcc tcgcggcctg gtacatgctg catcacacgc gtctggggcg ttacatctac
    28981 gcgctgggcg gcaacgaagc ggcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa
    29041 atcatcgtct attctctttg tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg
    29101 cgtctctcct ccgcacaacc cacggcgggg actggctatg agctggatgc tattgctgcg
    29161 gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc
    29221 ggcgcattaa ttcttggctt ccttaataat ggattgaatt tgttaggtgt ttcctcctat
    29281 taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag
    29341 cagtaataac gactacagga catcttgaat atgaacatga aaaaactggc taccctggtt
    29401 tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg
    29461 ctggtggtct ccacgcttaa caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa
    29521 gaggcggata aacttggcta taacctggtg gtgctggact cccagaacaa cccggcgaaa
    29581 gagctggcga acgtgcagga cttaaccgtc cgcggcacca aaattctgct gattaacccg
    29641 accgactccg acgcagtagg taatgctgtg aagatggcta accaggcgaa catcccggtt
    29701 atcactcttg accgccaggc aacgaaaggt gaagtggtga gccacatcgc atctgataac
    29761 gtactgggcg gcaaaatcgc cggtgattac atcgcgaaga aagcgggtga aggtgccaaa
    29821 gttatcgagc tgcaaggcat tgccggtaca tccgcagccc gtgaacgtgg cgaaggtttc
    29881 cagcaggccg ttgctgctca caagtttaat gttcttgcca gccagcctgc agattttgat
    29941 cgcaccaaag gcttgaacgt aatgcagaac ctgttgaccg ctcatccgga tgttcaggct
    30001 gtattcgcgc agaatgatga aatggcgctt ggcgcgctgc gcgcactgca aactgccggt
    30061 aaatcggatg tgatggtcgt cggatttgac ggtacaccgg atggcgaaaa agcggtgaat
    30121 gatggcaaac tagcagcgac tatcgctcag ctacccgatc agattggcgc gaaaggcgtc
    30181 gaaaccgcag ataaagtgct gaaaggagag aaagttcagg ctaagtatcc ggttgatctg
    30241 aaactggttg ttaagcagta gttttaatca ggttgtatga cctgatggtg acataaatac
    30301 gtcatcgaca gatgaacgtg taatataaag aaaagcaggg cacgcgccac cctaacacgg
    30361 tggcgcattt tatggacatc ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca
    30421 gcattaatgc tgaccacatt cttaatcttc aatcttttcc tactccaggc gaaaccgtaa
    30481 ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc gaatcaggcg gtggctgctg
    30541 ggcgtagcgg tgcgaatatc gcgtttattg cctgcactgg cgatgacagc attggtgaga
    30601 gcgttcgcca gcagctcgcc actgataata tcgatatttc tccggtcagc gtgatcaaag
    30661 gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga aggtgagaat gtcatcggta
    30721 ttcatgccgg cgctaatgct gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta
    30781 ttgccaacgc gtcggcatta ttaatgcagc tggaatcacc actcgaaagt gtgatggcag
    30841 cggcgaaaat cgcccatcaa aataagacta tcgttgcgct taacccggct ccggctcgcg
    30901 aacttcctga cgaacttctg gcgctggtgg acattattac gccaaacgaa acggaagcag
    30961 aaaagctcac cggtattcgt gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac
    31021 tgcatgaaaa aggtatccgt actgtactga ttactttagg aagtcgtggt gtatgggcta
    31081 gcgtgaatgg tgaaggtcag cgcgttcctg gattccgggt gcaggctgtc gataccattg
    31141 ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt gctggaagaa aaaacattgc
    31201 cagaggcgat tcgttttgcc catgcagccg ctgcgattgc cgtaacacgt aaaggcgcac
    31261 aaccttccgt accgtggcgt gaagagatcg acgcattttt agacaggcag aggtgacgct
    31321 tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg
    31381 ttatcaataa agatcgcttc gtcagtgaag cgattacagc caaagttgaa gcggcgatta
    31441 aagaactcaa ttacgcgcca tcagctctgg cgcgtagcct caaactcaat caaacacata
    31501 ccattggcat gttgatcact gccagtacca atcctttcta ttcagaactg gtgcgtggcg
    31561 ttgaacgcag ctgcttcgaa cgcggttata gtcttgtcct ttgcaatacc gaaggcgatg
    31621 aacagcggat gaatcgtaat ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt
    31681 tactgtgcac cgaaacgcat cagccttcgc gtgaaatcat gcaacgttat ccgacagtgc
    31741 ctaccgtgat gatggactgg gctccgttcg atggcgacag cgatcttatt caggataact
    31801 cgttgctggg cggagactta gcaacgcaat atctgatcga taaaggtcat acccgtattg
    31861 cctgtattac cggtccgctg gataaaactc cggcgcgcct gcggttggaa ggttatcggg
    31921 ctgcgatgaa acgtgcgggt ctcaacattc ctgatggcta tgaagtcact ggtgattttg
    31981 aatttaacgg cgggtttgac gctatgcgcc aactgttatc acatccgctg cgtcctcagg
    32041 ccgtctttac cggaaatgac gctatggctg ttggcgttta ccaggcgtta tatcaggcag
    32101 agttacaggt tccgcaggat atcgcggtga ttggctatga cgatatcgaa ctggcaagct
    32161 ttatgacgcc accattaaca actatacacc aaccgaaaga tgaactgggg gagctggcga
    32221 ttgatgtact catccatcgg ataacccagc cgaccctaca gcaacaacga ttacaactta
    32281 ctccgattct gatggaacgc ggttcggctt agatttacgc tgtcttttga tcaaattatt
    32341 accatcggtt gttttcagta gcatgaacat tgctgctgaa gcaacagtaa taatgcccat
    32401 cgtgataaac gtatagtgga attgttcgac tgtcgttgtg ccttccatcc cttcgtaaac
    32461 gcgaaggacg gccgcactta cagcaacgcc taaactgata gacagttgct gcgtgaccgc
    32521 cagaacactg ttaccgctgc tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt
    32581 catcgcggta aattgcgtcg acatagccat ccctaatata aacaacggca agatcagcat
    32641 ccatatcgcc attgctggtg attgcaaaga gaactgagcg atcattagcc caataatcac
    32701 cgtgatcccc actaacgtat ggcgatagcc cagacgacgt aagacttggg taaccatcga
    32761 ttttgcaata atggaaccta acgctgtcgg tgccatcata cagccagcaa taaacgcctg
    32821 atacccaaat cctacctgta acatcaatgg cataaggaac ggtacacagc cggtccccag
    32881 acgggttgca atattgccta cgataccgat cgagaaagtg cgggttttaa ataaatctaa
    32941 tgaaattaat gggtttggcg tgcgtcgcgc atggagaata tagagaagca gtaaccagat
    33001 gctggtgaca attaccgtca aggcaatcca gctggcgaca atcttttccc cgaatagctc
    33061 tattccgctt gagaagagaa caaggctgag gccaaacagc aaaaagccag tggtatcgaa
    33121 tctgcgtcgt gcggtggtga aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat
    33181 acctatgggg atattgatta aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc
    33241 gccaagaacg gggcctaaaa ttggccccac cagacccggc atggcgacaa aattcaatac
    33301 aggaagaagt tcattacgag gataagcgcg cagtaaggcc agccgagcaa caggcatcat
    33361 cattgcgccg cctatcccct gaataacccg gaagacaacc agctgtggta gcgagttgga
    33421 aagtgcgcag gccagagaac ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg
    33481 cgtaccgaag cgatcggcta gccatccgct taccggaata agcatcgcca ccgtcagcgt
    33541 ataactgatg atggctgatt gcatcgcgag aggagaacga ttaaggctat gagcgattgc
    33601 gggtaaggcg gtattcagaa tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc
    33661 gatccacggc aaacccgcca tactgcgctt ctttttatcg ctcattcaaa gtcctgttat
    33721 cgggttatca cttatctggt gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc
    33781 catcgctttg gataatcgca tcgacaatcg cctggtgcag atccagcttt atcactgtgt
    33841 cgctggtaat tgacgtgaag taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg
    33901 tcaaaaacgg attggcgctc atttcataga tatgctcatg ccaggccata tcgacttcga
    33961 tccagcgttc acggcgaaag ttctctttta atgccgccat ttcggccatt aacgtattga
    34021 gatgcgcctt ctgttccgcg gtgccaaccg ttgctgccag taggcaagct tgcggctcca
    34081 gacagatacg cataaccaga aagtgatcga tgacctgatg aaagttctct tctgtcatcc
    34141 accaggtaag caattcctga tcaagaaaat tccagttcga ttgtggcatg actcgggtac
    34201 caattcgcgg tcgcggtaaa accatccctt ttgccgttaa cgttttgacc gcttcgcgta
    34261 ccgctgtacg actcactcca aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc
    34321 cgggttcata ttcacctttt aagatccgct gcgccagctt ttcagccaga acatacgaaa
    34381 ggtttttctg tgcagctaac tgttgtgcgc ttaaaggcat tactaatctt cctttttctt
    34441 tttattcctc cttagtatgc caccaggaag tgtgattacg gttgcaaaaa cggcaaattg
    34501 cttgttttat ggcacattaa cggggctttt gctgaaaaaa tgcgcggtca gaaaattatt
    34561 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
    34621 caacggcaaa cacgccgccg ggtcagcggg attctcctga gaattccggc agagaaagca
    34681 aaaataaatg cttgactctg tagcgggaga gcgtattatg cacaccccgc gccgctaaga
    34741 aaaaacgaag cggcactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag
    34801 atacggattc ttgacgtcgc aagacgaaaa atgaatacca agtctcagga gtgaacacgt
    34861 aattcattac gaagtttaat tctttgagca tcaaactttt aaattgaaga gtttgatcat
    34921 ggctcagatt gaacgctggc ggcaggccta acacatgcaa gtcgaacggt aacaggaaac
    34981 agcttgctgt tt
//
LOCUS       DAJOTB010000033        34718 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJOTB010000033 DAJOTB010000000
VERSION     DAJOTB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.033"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            22..762
                     /locus_tag="OHN28_001645"
     CDS             22..762
                     /locus_tag="OHN28_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2297053.1"
                     /translation="MFFNTKYTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     VLPKDRESVFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPL
                     ALKWSWSGSEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTG
                     LNGKANSAKVHFYAINDFGGAIEGNASL"
     gene            797..3397
                     /gene="htrE"
                     /locus_tag="OHN28_001646"
     CDS             797..3397
                     /gene="htrE"
                     /locus_tag="OHN28_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCQ2297054.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3414..3986
                     /locus_tag="OHN28_001647"
     CDS             3414..3986
                     /locus_tag="OHN28_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2297055.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            4001..4603
                     /gene="yadL"
                     /locus_tag="OHN28_001648"
     CDS             4001..4603
                     /gene="yadL"
                     /locus_tag="OHN28_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCQ2297056.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4630..5226
                     /locus_tag="OHN28_001649"
     CDS             4630..5226
                     /locus_tag="OHN28_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2297057.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            5278..6531
                     /locus_tag="OHN28_001650"
     CDS             5278..6531
                     /locus_tag="OHN28_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCQ2297058.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VAVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6644..7438
                     /gene="panB"
                     /locus_tag="OHN28_001651"
     CDS             6644..7438
                     /gene="panB"
                     /locus_tag="OHN28_001651"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCQ2297059.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7450..8301
                     /gene="panC"
                     /locus_tag="OHN28_001652"
     CDS             7450..8301
                     /gene="panC"
                     /locus_tag="OHN28_001652"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCQ2297060.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8383..8580)
                     /locus_tag="OHN28_001653"
     CDS             complement(8383..8580)
                     /locus_tag="OHN28_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2297061.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLALRLLSKGM
                     SREDVAEMANLPLAEIDKMIN"
     gene            complement(8649..9569)
                     /locus_tag="OHN28_001654"
     CDS             complement(8649..9569)
                     /locus_tag="OHN28_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2297062.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEVRQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9843..10223
                     /gene="panD"
                     /locus_tag="OHN28_001655"
     CDS             9843..10223
                     /gene="panD"
                     /locus_tag="OHN28_001655"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCQ2297063.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10227..11456)
                     /locus_tag="OHN28_001656"
     CDS             complement(10227..11456)
                     /locus_tag="OHN28_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCQ2297064.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGLGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTQVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11520..11960)
                     /locus_tag="OHN28_001657"
     CDS             complement(11520..11960)
                     /locus_tag="OHN28_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2297065.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12065..12835)
                     /locus_tag="OHN28_001658"
     CDS             complement(12065..12835)
                     /locus_tag="OHN28_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297066.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12832..13758)
                     /locus_tag="OHN28_001659"
     CDS             complement(12832..13758)
                     /locus_tag="OHN28_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2297067.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13867..14529
                     /gene="can"
                     /locus_tag="OHN28_001660"
     CDS             13867..14529
                     /gene="can"
                     /locus_tag="OHN28_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCQ2297068.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14570..15106)
                     /gene="hpt"
                     /locus_tag="OHN28_001661"
     CDS             complement(14570..15106)
                     /gene="hpt"
                     /locus_tag="OHN28_001661"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCQ2297069.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15312..17702
                     /gene="gcd"
                     /locus_tag="OHN28_001662"
     CDS             15312..17702
                     /gene="gcd"
                     /locus_tag="OHN28_001662"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCQ2297070.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLSVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17749..19299)
                     /gene="cueO"
                     /locus_tag="OHN28_001663"
     CDS             complement(17749..19299)
                     /gene="cueO"
                     /locus_tag="OHN28_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCQ2297071.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRNLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19465..19812
                     /locus_tag="OHN28_001664"
     CDS             19465..19812
                     /locus_tag="OHN28_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCQ2297072.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19918..20784
                     /gene="speE"
                     /locus_tag="OHN28_001665"
     CDS             19918..20784
                     /gene="speE"
                     /locus_tag="OHN28_001665"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCQ2297073.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20800..21594
                     /gene="speD"
                     /locus_tag="OHN28_001666"
     CDS             20800..21594
                     /gene="speD"
                     /locus_tag="OHN28_001666"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCQ2297074.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21632..21994)
                     /locus_tag="OHN28_001667"
     CDS             complement(21632..21994)
                     /locus_tag="OHN28_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCQ2297075.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22169..24766)
                     /gene="acnB"
                     /locus_tag="OHN28_001668"
     CDS             complement(22169..24766)
                     /gene="acnB"
                     /locus_tag="OHN28_001668"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCQ2297076.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25121..26884
                     /locus_tag="OHN28_001669"
     CDS             25121..26884
                     /locus_tag="OHN28_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCQ2297077.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(26955..28379)
                     /gene="lpdA"
                     /locus_tag="OHN28_001670"
     CDS             complement(26955..28379)
                     /gene="lpdA"
                     /locus_tag="OHN28_001670"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCQ2297078.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28587..30479)
                     /gene="aceF"
                     /locus_tag="OHN28_001671"
     CDS             complement(28587..30479)
                     /gene="aceF"
                     /locus_tag="OHN28_001671"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCQ2297079.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30494..33157)
                     /gene="aceE"
                     /locus_tag="OHN28_001672"
     CDS             complement(30494..33157)
                     /gene="aceE"
                     /locus_tag="OHN28_001672"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCQ2297080.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33318..34082)
                     /gene="pdhR"
                     /locus_tag="OHN28_001673"
     CDS             complement(33318..34082)
                     /gene="pdhR"
                     /locus_tag="OHN28_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCQ2297081.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(34538..>34718)
                     /locus_tag="OHN28_001674"
     CDS             complement(34538..>34718)
                     /locus_tag="OHN28_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCQ2297082.1"
                     /translation="LDKYWDDLEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVK
                     EWRRSQGLPLFKDSE"
BASE COUNT         8689 a         9042 c         8311 g         8676 t
ORIGIN      
        1 taactctacc aggaatggtt tatgtttttc aacactaaat atacagcagc attatgcttc
       61 gcaacctgtg tagcttttag ctcgtcagct atagcagata tcgtaatttc cggtacacgc
      121 gtaatctata aaagcgacca aaaaagtgtc aacattcgcc tggaaaataa aggcaataac
      181 ccattacttg ttcagagttg gctggatact ggtgatgaca atgctgagcc tggcagtatt
      241 accgtcccct ttactgcgac tccgcccgta tcacgcattg atgcaaaacg cggccaaacc
      301 atcaaactga tgtacaccgc cagcagcgtc ctgccaaaag acagagaaag tgtgttctgg
      361 tttaacgtac tagaagttcc tccaaaacca gatgccgcaa cagttgcaaa tcaaagcctg
      421 ctgcaattgg catttcgcac acgtataaaa ctgttctatc gcccggaagc attgaagggg
      481 aatccctcag aagcaccatt agccctgaaa tggtcctggt caggttccga gggtaaagca
      541 gcattacgag tggccaatcc aaccccttac tacgtctctt ttagcagtgg tgatttagaa
      601 gccagcggta aacgctttcc gattgatgtg aaaatgattg caccattcag tgatgaagtc
      661 atgaaagtca ctggccttaa tggcaaagca aattctgcaa aagtgcattt ttacgccatt
      721 aatgattttg gtggtgccat tgaaggtaat gccagtctgt aatcaggcaa ggatataatt
      781 ccgcaggaag cataacgtga ctataaaacc aactaaaaat tatcacaatc atctgacccg
      841 tatcgccacg ttttgcgcgc tgctgtattg caatggtgct ttctgtgctg aacttgttga
      901 atatgaccat accttcctga tggggcagaa tgcctcaaat attgatctca gccggtatag
      961 tgaaggtaac cccgctatac cgggtgtgta cgacgttagt gtttatgtca acgatcaacc
     1021 tgttattaac caaagcatta catttgtcgc aattgaagga aaaaagaacg cacaagcctg
     1081 tatcacactt aaaaatttat tgcagttcca tattaattct cccgatataa acagcgaaaa
     1141 agccgttctg cttgcccggg atgaagctct cggcaattgc ctcaatttga cggaaattat
     1201 ccctcaggct tccgttcgtt atgacgttaa cgatcaacgt ctggatatag acgttcctca
     1261 ggcctgggta atgaaaaatt accaaaacta tgttgatcca tcgttatggg aaaatggcat
     1321 taatgcagcc atgttgtcat acaacctcaa cggatatcat agtgaaaccc ctggtcgaaa
     1381 aaatgacagc atttatgctg cttttaacgg tgggatgaat ttgggtgcat ggcggctgcg
     1441 tgcctcaggc aactacaact ggatgaccga ttctggcggt aattatgatt ttaagaatcg
     1501 gtatatacag cgtgatatcg cctcactacg ctctcaattc attattggtg agtcttatac
     1561 gacgggcgaa acctttgatt ccgtcagtat ccgaggcatt cgcttataca gtgacagccg
     1621 catgttgcct ccgacattag ccagctttgc gcctatcatt catggcgttg ccaataccaa
     1681 cgccaaagtc actattacgc aaggtggcta taagatttat gaaacgacgg tgccgccagg
     1741 cgctttcgtc attgatgatc tgagtccatc agggtacggc agtgatctta ttgttaccat
     1801 cgaagaatcc gatggctcaa agcggacatt ctcgcaacct ttttcatcgg tcgttcaaat
     1861 gttacgccct ggcgttggac gttgggatat tagcggcggc caggtcttaa aagatgatat
     1921 tcaggatgag cctaatttat ttcaggccag ctactactac ggtctgaata actatctgac
     1981 gggttatacc ggtattcaga ttaccgataa taactatacc gctggtttgt taggtcttgg
     2041 tctgaatact tcggtcggtg cattttcttt cgatgtgact cactccaatg ttcgtatacc
     2101 ggatgataaa acataccagg ggcaaagtta tcgtgtttcc tggaacaagt tattcgaaga
     2161 aacaagtact tcactgaata tcgcggccta tcgctattcg acacagaatt atcttggtct
     2221 taatgatgca ctaactctga ttgatgaagt gaaacatcca gaacaagatc ttgaaccgaa
     2281 atccatgcgt aattactcgc gcatgaaaaa tcaggttaca gtcagtatta accaaccgtt
     2341 gaaatttgaa aaaaaagatt acggttcttt ttatctttct ggtagttggt ccgattactg
     2401 ggcttccgga caaaatcgta gcaattactc cattggctac agtaacagtg catcctgggg
     2461 cagctacagc atcagtgcac agcgttcatg gaatgaggac ggcgacgctg atgatagcgt
     2521 ttatctcagt ttcaccattc ctattgaaaa attacttggc actgaacaac gtacttcagg
     2581 tttccagagt atcgatactc aaatgagcag tgactttaag ggtaataacc aactcaacgt
     2641 cagtagcagt ggctatagcg ataacgctcg cgtcagttat agcgtgaata ctggctatac
     2701 gatgaataaa gccagcaaag atttgagtta tgttgggggt tatgccagct atgaatcacc
     2761 gtggggaact ctggcaggtt cagtttctgc aaacagcgat aacagtcgtc aagtttctct
     2821 cagcaccgac ggtggttttg tattgcatag tggcggcctg accttcagta atgacagttt
     2881 tagcgactcc gatacactgg cggtagttca ggctccaggt gctcaaggag cgcgaataaa
     2941 ttatggcaac agtactatcg atcgatgggg ttatggtgtc accagcgctc tttctcctta
     3001 tcatgaaaac cgtatcgcgc tggatatcaa cgatcttgag aacgatgttg aattaaaaag
     3061 taccagtgct gtagctgtgc ctcgtcaggg ttctgtcgtt tttgctgatt ttgaaaccgt
     3121 gcaagggcaa tcagccatta tgaacatcac acgtagtgat ggcaaaaata ttccatttgc
     3181 tgcagatatt tatgatgacc aaggcaatgt cattggtaat gttggacagg gtggacaggc
     3241 ttttgttcgt ggtattgaac agcaaggaaa tatcaacatt aaatggctcg aagaaagtaa
     3301 acctgtaagt tgtcttgcgc attatcaaca aagttcagaa gcagaaaaaa tagcgcaatc
     3361 tattattctg aatggaattc ggtgtcagat tcagtaacta caagggacgt caaatgataa
     3421 aaacaacgcc acataaaata gcgatactga tgggagtatt attatcaccc tcagtatttg
     3481 caacggatat taatgttgag tttacagcca ctgtcaaagc gacaacctgt aacatcacac
     3541 ttactggtaa taatgtcacg aatgatggca ataataacta cactttgaga atccctaaga
     3601 tgggcctgga taagatcacg aataaaacga cagaatctca ggctgatttt aaactggttg
     3661 ccagtgggtg cagcagtggc attagctgga ttgataccac tctgaccgga aacgcattat
     3721 caagctcatc caagcttatt ataccgctgt ctggtgattt atcttcgacg acaagtaata
     3781 tcggtatggg tttcaaaaaa cggactactg atgatgccac tttccttcaa cctaacaaca
     3841 gtgcggacaa gatacgctgg agcacagaag aaatgcagcc cgataaaggt cttgaaatga
     3901 ctgtcgcgct acgtgaaacc gcagcagggc aaggcgtgcc ggggattttt cgcgccctgg
     3961 cgacgtttaa tttcatctat caataagaag gataattaag atgacattta aaaatttacg
     4021 ttatggatta tccagcagtg tcgttttggc tgcctcattg ttcagcgtac cctcttatgc
     4081 ggcaactgac agcattgggc tgaccgtgat taccaccgta gaaatgggta cttgtaccgc
     4141 tacattagta aatgactctg atcaggacat ttctgttgtt gattttggtg atgtatatat
     4201 ttctgaaatc aatgccaaga ccaaagtaaa aacattcaaa ctcaaattca aagactgtgc
     4261 gggtatcccc aataaaaaag cgcaaataaa attaaccaag cgagccacat gcgaggggac
     4321 tgctaatgac ggtgcagggt tcgcaaatgg ttccacagcc acagataaag caagtgccgt
     4381 cgccgttgaa gtctggagca ccgaaactcc ggcaacaggg agagcaacac aatttagttg
     4441 tgtgacacca gcatctcaag aggtaactat ttccactgct gccaatgcgg ttgtttatta
     4501 cccgatgagt gctcgcctgg tcgttgaaaa aaataaaacc gtaagtaatg tcactgcagg
     4561 taaattttct gcacctgcca catttacagt aacctataac taacagactg acagattcag
     4621 gaactatata tgcatcccac tcaacgtaag ctgatgaaga gaataattct gtttctgtca
     4681 ttactgtttc ccattacctg tccagcgatc gctggacagg atattgacct tgttgctaat
     4741 gtaaaaaaca gcacttgcaa aagcggaatc agtaaccagg gtaatattga tcttggtgtc
     4801 gttggggtgg gatatttttc aggtaatgtt actcctgaaa gctatcaacc aggtggaaaa
     4861 gagttcactg tcactgtctc agactgtgca ttacagggaa ctggcgatgt gctaaatcag
     4921 ttacatattg attttagagc ccttagcggt gtcatggctg caggctccag gcaaatattt
     4981 gccaatgaag tgacaaccgg ggctaaaaat gtcggtgtcg ttctcttttc cattcaggac
     5041 ccggccaata tattcaatgt tcttagttca gcgggaaatt ctcgttcaat ctatccagta
     5101 atgacttctg ccctgaataa ttcgtcatgg aaattttatg ccagaatgca aaaaatcgat
     5161 cccgcgctgg atgtcatatc tggccaggtg atgagtcata tattagttga tgtgcactac
     5221 gaataatata tcaaaacaaa cgactgctat tatgacagca gcgaatagtg ggtaatcatg
     5281 aagacattct tcaaatactt tgttttttta gtactatcct gctcttgctt tacagcgatt
     5341 agtaaggata caagttttct tgttggtaac aatgccgggg taggcgatgg taatttcaac
     5401 ggcccgagca ctgcggctca ggtcactttt agatatgcat caacaaccaa taatttagtt
     5461 ttttataaac ctactcaact tggcccgaca ggtgttaaat tacgctggga acaattagat
     5521 acagccagcg gtggcggttt tctttactgt aatgcctctg acagggcaaa tccagatgga
     5581 ctaatggata ttgaaaatgc catggctgat tctggtaaaa cctatggtgg acataagtta
     5641 tttaaaacct ctgtcccagg cttgtattac actctattta ttgcaaaact ttggtctgca
     5701 tattcaacaa aaacaactat tagcgactct ggtttatata ttggagatac cacaccacaa
     5761 aaattccagt ggatcatcac agaccctgat ctacaacata tcggctgcga taacgcgatg
     5821 cggtacgata gattctgggc catcggcggt gttgtacacg atcttacgat tgaattttat
     5881 actgataccg attttaaccc tacaacaaac caggacgtat cattgtccag taattcgaca
     5941 tatctttatg cgtttaaggc ctacagtccc ggttctcgtg ttgttgatca cagccatcac
     6001 ctttatatcg actttgatct actcaatgtt aaattaacta accccacctg ctttactgct
     6061 gtacttaccg gaaaatcagt aagtggttcg acggtaaaaa tgggagaata tgcaccaggg
     6121 cagattaaaa atggtgctac tccggttcct tttgatattt ctctccagaa ctgtgttcgc
     6181 gttggcgata tagagacaaa gctatcttca gggaagctgg gtacagaaaa taagcaactc
     6241 ctgggtaaca ctcttactgg aagcggcgca gcaaaaggcg ttgcggtatt gattgaagga
     6301 ttagcaaaca gaaaaagtgc cctaatgata ctgaaaccga atgattcaac ttctgtttat
     6361 aaagataata cgggtcaggc tcaggatcac gattcagatg caatttaccc agaagatcaa
     6421 ggtataacat atccacttca tttccaggcg acgttaaagc aagacggaaa tattgctatc
     6481 gaaccaggag aatttaaagc caccagtact ttccaggtaa cctatccctg ataacattgt
     6541 ggcaccgtct ggtaatggcg gtgccacaat agataaatca ttgcccccac caatgtggct
     6601 gataaaatgc cagacagcac gacattactt atcaggatac gttatgaaac caaccactat
     6661 tgcttcattg caaaaatgca aacaagaaaa aaaacgtttc gcgaccatca ctgcttacga
     6721 ttatagcttc gcaaaactgt ttgccgaaga aggacttaac gtcatgctgg tcggcgattc
     6781 tctgggcatg acggttcagg ggcatgactc cacgctaccg gttaccgttg ctgatatcgc
     6841 ctaccacact gccgccgtgc gtcgcggcgc gccaaactgc cttctgttag cagacctgcc
     6901 atttatggcg tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg taatgcgtgc
     6961 cggagccaac atggtcaaaa ttgaaggcgg tgagtggctg gtagaaaccg tacaaatgct
     7021 gaccgaacgt gccgttccag tttgtggtca tttaggccta accccacagt cagtaaatat
     7081 tttcggtggc tacaaagtcc aggggcgcgg cgatgaagcg gccgatcgac tgctcagcga
     7141 tgcattagcc ttagaagctg ctggggcaca actgctggta ctggagtgcg tgccggttga
     7201 actggcgaaa cgcattaccg aagcactggc gatcccggtt atcggtattg gcgcaggcaa
     7261 cgtcactgac gggcaaatcc tcgtgatgca cgacgcgttc ggcattaccg gcggtcatat
     7321 tcctaaattc gccaaaaatt tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca
     7381 gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt tccattgagg
     7441 agtgacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat ccgccgcctg
     7501 cgtatggaag gtaagcgcgt ggcgctggtg cccaccatgg gtaacctgca cgacggccat
     7561 atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt
     7621 aacccaatgc agtttgaccg cccggaagat ctggcacgtt atccacggac cttgcaagag
     7681 gactgcgaaa agctgaacaa acgtaaagtg gatttagttt tcgcgccttc ggtaaaagag
     7741 atctacccga acggtacaga aacccacacc tatgttgatg ttcctggtct ttccaccatg
     7801 ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgactattgt cagcaagctg
     7861 ttcaacctgg tccagccgga catcgcttgc ttcggcgaaa aggactttca gcaactggcg
     7921 ctgatccgca aaatggttgc cgatatgggc tttgatattg agattatcgg cgtaccgatt
     7981 atgcgcgcca aagacggtct ggcgctgagt tcccgtaacg gttatctgac ggcggaacaa
     8041 cgcaaaattg cgcccggtct gtacaaggtt ttaagttcga ttgccgacaa attgcaggct
     8101 ggcgaacggg atctcgatga aattattgcc attgccgggc aagaactgaa tgaaaaaggc
     8161 ttccgcgccg atgatattca gattcgtgat gccgacacat tgctggaagt ctctgaaaac
     8221 agcaaacgcg cagtgattct ggtagccgcc tggcttggcg atgctcgcct gatcgacaac
     8281 aaaatggtcg aactggcgta atgcttaact ggcgctacgg ctgatggcgc cagctattca
     8341 ttgccacgtt atctgtaagc gattattcat aacgcagaca acttagttaa tcattttatc
     8401 aatctcagca agaggtaaat ttgccatctc tgcaacgtct tcccgagaca ttcctttact
     8461 cagaagacgc agggccagtc ttaccttctg gagtccctct ttatatccct tctgaaatcc
     8521 cttctcttca aaccactgcg ccaacgtctg cctggtttct ctccgtgtct ttatttccat
     8581 atgttccctt aattatctgg tcagttaaca ataacaatta tctggctata actgataatg
     8641 cagataactt agctaatcag cttatcaacc tcagcaagag gtaaatttgc catctctgca
     8701 acgtcttccc gagacatccc tttactcaga aaacgcaggg cgaattcctg tcttacttcc
     8761 tgtctaacct cttgtattac ctcctgtctt acctcctgtc ttacctcctg ccttcccctc
     8821 tcttcaaacc actgcgccag cgtcatcata gactcccctc ccgtttccct gtctcgcaac
     8881 acaccataaa acaaatccgc ttgttcagta tgaccgcgtt gcagcatata gttttgcatg
     8941 gcaactaact gacttccgct agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc
     9001 aataacatta agtcgcgctg gcgaatatgc ttttgcagta gttcgagaat cgcaatccgc
     9061 cggtgctgca tgatttcgtc atccggcgtg atggtgatat ccaccagcgg gaaagggttg
     9121 ttgtaaacgc gtcgcgccag ctccggcgag taaaacatat caaaccagca cattgagagc
     9181 ggataaggtg tggcctcgcc ctgataaaac agaatcggca ccaccagcgg cagcttatcg
     9241 tgatccgcct ccagatgccg gtgcatggcg gcaatggcat agcgcatcat gcgaaaggtc
     9301 attttcttat ccggcttgct ttggtgttca atcacaacat gcagataacc cgtactaccc
     9361 tgcatctgta cggaatagag cacgtcagtg ctgtgccctt tcaggctttc ttcaataaaa
     9421 ctccccgact caagatgcag cgtgtcgagg tcacacagtt cgcgtagctc cgctggcaaa
     9481 tggatatcca gaaagtcgcg agccgtttcc gcatgcatta aaaattgttt aaacaccgca
     9541 tcatgcggtg tggtactcgg tgcatccatc tcccctgcct tccgtgttgt cttgcgtgaa
     9601 ggcagtatcc agtcaaggtg tgccgtcgtc agccgcctct ttagtgttta ccgagcagcg
     9661 ttcagataaa tttcaattgg ttacaggcgt tacatggcac gcttcggcac ggtacgcaaa
     9721 ctgctgatgc aaatgccgtc agatgagtag acactaaaca aaaatcgggc aatactgcgt
     9781 gagaatttcc tcaaagcagg catcgcctgc ttcgttaacg acagggtaga aaggtagaag
     9841 ttatgattcg cacgatgctg cagggcaaac tccaccgcgt gaaagtgact catgcggacc
     9901 tgcactatga aggttcttgc gccattgatc aggattttct tgacgcagcc ggtattctcg
     9961 aaaacgaagc cattgatatc tggaatgtta ccaacggcaa gcgtttctcc acctatgcca
    10021 tcgcggcaga acgcggttcg agaattattt ctgtcaacgg tgcggcggcc cactgcgcca
    10081 gcgtcggcga tattgtcatc atcgccagct tcgttaccat gccagatgaa gaagctcgca
    10141 cctggcgacc caacgtcgcc tattttgaag gcgacaacga aatgaaacgt accgcgaaag
    10201 cgattccggt acaggttgcc tgattgttat ccctgcggct ggttactcac cagccgcgac
    10261 atcgtctcca gcgaatctgt tcttaagata taaagtcgtt ttagtaacaa cggattatcc
    10321 cccggtttta ctttgccttt catggtcgtc acagccaggt gaaatccggc ttcttttgct
    10381 gccttcacgg ctttgtcatt aaatccgcca aatggataag aaagatacaa aacatgtgga
    10441 ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta
    10501 cggctcagca atataggccg ccgataacca tctatccgat gcaaaaaatg agtatgtgac
    10561 tggaaatcaa atacatcgcg aatttcgtta agttcagaaa cgctcataaa ttgcagcgat
    10621 tttgggttcc atttctgcgg gtgacgtttg atgcgcgagg taacaataaa cgccgtcgcc
    10681 ttcatgccat attgtttcaa cacaggatac gcatagcggc tcaccgactt gaggccatca
    10741 tcaaaggtaa tcaccaccgc tcgcgcaggg agattgatct tattcttcac gtaaccttcc
    10801 agctgcgcca tgctcagtgt cgcgtatccc ctgtcgcgca gccaggccat ctggttattg
    10861 aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac gggtgttttc ttcatcgcgc
    10921 aaaatatggt gataggttag caccggcagg ccattatcgg gttgggcatc cagcgcgctg
    10981 atataggcca gtcgatcgcc aatacggatc tgataccagg tttgatttaa cctgtctttc
    11041 agtttatgca aaatcgggta gcgcaaattg tccgccagta ctccaaatgg cgcactgccc
    11101 acactcggcg cgttatagac ctgcgtatct ttccaggtaa ctaagttctg attactcagc
    11161 ggcttgttga ggtcacccaa accgtcttca actttttgtc gcccctgaac cggctcgaga
    11221 tgccctttat cgataaaccc tttgccaagg ccaaaattaa atacgtaata acttgcggca
    11281 gtgggctcca cggcaatgat ttgtccggcc cgaatattcc ccaccgtcac catcttgtca
    11341 ccaatttgcg cccagaccgc ggcattttcg atggtttgca tataacgggc aggtaacgcg
    11401 gcactgacac ttgcggtaaa cagcatcagc aggagaataa cagcttgttt gtacatgaaa
    11461 aaataaccga gggtgaaaca acgcttttat tgtagcaaaa gcgtcatcag aatcgcgggt
    11521 taatactcat acaaattatg tttgaggacg aaaggcggat ttttttgttg ctggtgccaa
    11581 agactactca cctccggcgc acccagttcg acaatacgct cgcgaaacgc tgaactggtc
    11641 atggtttcac ggcaagccat catctgttca attaacggta acgtgacacc agaaatcact
    11701 tcgcaacggg agtgtttgtg gcttaataat gaagccacgc ggtacggtgg cgctccggct
    11761 atatcggtta agaaaataac gccttcacca gagtccgttt catgcaaggc atcgcacatc
    11821 atgcggctga gcatattaga gcttaatccg cgccagaaat tcacggcccg gcactgaaga
    11881 agtgccccat gttttttctc cagcgcatcc agtatctctt gcgcccggtc atcgtgacag
    11941 gtaattaccc aacctaacat cgccttcctc cttagccgaa aatcagtcta ccctgaccat
    12001 tgttgataaa gggtgatagc cgtcaaaact cctctccccc aaatccagag gagaggaaat
    12061 agccttagct acgcaaacca cgtccacgtt ggatcagcga ccaacagatc aaataaaacg
    12121 ccacaataaa gaccaccagt acgccaaagg tagtgaccag cggaacatca ttgataccga
    12181 ggaagccgta gcggaaacca ctgatcatat aaacgattgg gttcaggtgc gacagccctt
    12241 gccagaacgg cggcaacaaa gtcagggagt aaaacacccc gcccaaatac gtgagtggcg
    12301 ttaacacaaa ggttggcacc aggctgatgt catcgaacgt tttagcaaac acgccgttca
    12361 gcaaacccgc aagggagaat agcaccgcag tgagcaccag ggttaaggca acgaataccc
    12421 acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc cgtcaccaga ataccaacaa
    12481 acagaccacg cgccacgcca ccaccgacat atccggcaat aatgacatga gtcggaaccg
    12541 gcgctaccag cagctcttca atattacgct ggaacttggc accaaaaaat gatgacgcga
    12601 cgttagcgta ggcgttggtg atcaccgaca tcatgatcag ccccggtacg atgaactgca
    12661 tgtagctgaa gccatgcata tcgccaatac gcgaaccaat caggttaccg aagataataa
    12721 agtaaagggt catggtgatg actggcggca ccagcgtctg cacccagata cgcataaagc
    12781 gatggatctc tttcgcccag atgcttttta gcgccaccca gtaaagatgc atcatgtgcg
    12841 atctccttgt ttttcattaa ccagtgaaac aaacagctct tccagacggt tagctttgtt
    12901 acgcatactt aatacctgaa tgccctgctc acttaactgc gtaaatacgc tgttgatgcc
    12961 ctgctcacgc agcacttcaa cttccagcgt ggcggtatct accagtcgat actgatagcc
    13021 atcgagcttc ggtaacggac ttttcggtgc gagatcgaga ataaaggttt ctgatttcag
    13081 cttcgccagc agcgccttca tcgaagtatt ttccaccaac tcaccgtgtt gaataatgcc
    13141 gatattgcgg cacagcattt ctgcttcttc caggtagtgt gtggtgagaa tgatggtggt
    13201 gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag cggcgaagtt caatatccac
    13261 gcctgcggtc ggttcgtcga gaatcagtag tttaggttca tgcattaacg cacgggcaat
    13321 cattaaacgg cgcttcatcc cgccagataa catacgcgca cgttcgttgc gttttcccca
    13381 tagatcgagt tgtttaagat acttttcgct gcgaatgtac gcttctttgc gctccacacc
    13441 gtagtaccct gcctgattca ccacaatttg ctgcacggtt tcaaacgggt tgaagttaaa
    13501 ttcctgtggc accagaccca actgacgttt agcgtttacg acatctttct cgagatcgta
    13561 accaaatacg ctgacccgcc cggaggtttt atttaccaga gagctgataa taccgatagt
    13621 ggtcgatttt ccggccccgt tcggcccgag aagcgcataa aaatcacccg cttcgacctg
    13681 caaatcgatt ccacgaagcg cctgaacgcc gcctggatag gtttttttaa gctgttgaag
    13741 ttccagtgca atggtcataa atttttactt accttacgtt cttacacttt atctgtggtt
    13801 taaatcgtcc cggagttgcc ctatattagc caaacgtaat tatttggtta caggtcgtta
    13861 acctccatga aagacataga tacactcatc agcaacaatg cactatggtc aaaaatgctg
    13921 gtggaagagg atcccgggtt ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta
    13981 tggattggat gttccgacag tcgcgttcct gcagaacgtt taaccggtct tgagccgggc
    14041 gaactctttg ttcaccgtaa tgttgctaac ctggtcattc acaccgacct gaactgcctt
    14101 tccgtggttc agtatgcagt ggatgtactc gaagttgaac acattattat ctgtggccac
    14161 tacggttgcg gcggcgtaca agccgcagtt gaaaacccgg aactggggct tatcaacaac
    14221 tggctgctgc atatccgcga tatctggttc aaacatagct cattgctcgg cgaaatgccg
    14281 caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg
    14341 ggccactcca ccattatgca atcagcgtgg aaacgcgggc agaaagttac cattcacggc
    14401 tgggcctacg gcattcacga cggcttgctg cgtgatctgg atgtcacagc gactaaccgc
    14461 gaaacccttg agcaacgtta ccgtcacggg atctccaacc tcaagctgaa acacgccaac
    14521 cacaaataaa aatgccatgc cggatgcaac acatccggca acttcacact tactcgtcca
    14581 gcagaatcac tttgccgata tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt
    14641 aacccaccac aaactcatcc gggatcgaga aaccgataaa ttctaccggg acgttcactt
    14701 cacgacggga cggtttatcc agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc
    14761 ttaagatctc acgcactttc gacagtgtat tccccgagtc gatgatatct tcaacaatca
    14821 gcacgtcctt gccacggata tcttcatcca gatctttgag gattttcaca tcacgggtgg
    14881 tggacatgcc gctaccgtag ctggaggcgg tcataaagtc gacttcatga gatacctgaa
    14941 cttcacggca caggtccgcc ataaacataa atgagccacg cagcagaccc accagcacca
    15001 tatcgctgcc gctgtctttg taacgctcag taatctgacg acccagttcg gcgatacgcg
    15061 ctttaatctc cgcttcgggg atcattactt ctacagtatg tttcatatct ataaccatat
    15121 gatttaaaaa caaatcactt cgcacgggca ggttttggca ccgcaacaaa gcgcaagaca
    15181 cgcagtatac cagtaaaact attcattcgc ggaatctgtt aataaagctc gaattgtgat
    15241 gacgatcaca catgttaaaa cctataatta attgctacta aaatattaat gaattgaaat
    15301 ggtgtctctt tatggcaatt aacaataccg gctcgcggcg attactcgtc acgctaacag
    15361 ccctttttgc agcgctttgc gggctgtatc tactcattgg cggaggctgg ctggtcgcga
    15421 ttggcggctc ctggtactac cctatcgctg gccttgtgat gctcagcgtc gcctggatgc
    15481 tgtggcgcag taaacgcgcc gcgctttggc tgtacgcagc cctgctgctc ggcactatga
    15541 tttggggcgt ctgggaagtt ggtttcgact tctgggcgct gactccgcgc agcgacattc
    15601 tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg
    15661 ccagcggcgc agttgccgca ctggtggtcg cactgctgat tagcggtggt atcctgacct
    15721 gggccggatt taacgatccg caggaaatca gcggcacctt aagcgccgat accacgccag
    15781 cagaagccat ctcccccgtt gccgatcagg actggcctgc ctatggtcgc aatcaggaag
    15841 gtcaacgctt ttcacctctg aaacaaatta acgctgataa cgtccacaag ctgaaagaag
    15901 cctgggtgtt ccgtactggc gatgtgaagc agccgaacga tccgggcgaa atcaccaatg
    15961 aagtgacgcc aattaaagtg ggcgacaccc tttacctgtg taccgcgcac caacgtctgt
    16021 ttgcgctgga tgccgccagc ggtaaagaga aatggcatta cgatcctgag ctgaaaacca
    16081 acgagtcctt ccagcatgta acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa
    16141 ctgcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat cattcttccg gtcaatgatg
    16201 gtcgtctgat tgcaattaac gctgaaaacg gcaagctgtg cgaaaccttc gccaataaag
    16261 gcgtgctcaa tctgcaaagc aatatgccgg acaccaaacc gggtctgtat gagccgactt
    16321 cgccgccgat tatcaccgat aaaaccatcg tgatggccgg ttcagtcacc gataacttct
    16381 caacccgtga aacatctggc gtgatccgtg gttttgatgt caacaccggg gagctgctgt
    16441 gggcttttga tccgggcgcg aaagatccga atgcgatccc gtctgacgaa cataccttta
    16501 cctttaactc accaaactcg tgggcaccag cggcctatga cgcgaagctg gatctggtct
    16561 atctgccgat gggcgtcacc acgccagata tttggggtgg taaccgcaca ccggaacagg
    16621 aacgttatgc cagctcgatt ctggcgctga atgccactac cgggaaactg gcgtggagct
    16681 accagaccgt tcaccacgac ctgtgggata tggatcttcc ggcacagccg acgctggcgg
    16741 acatcaccgt taatggtcag aaagtgccga ttatttacgc tccggcgaaa accggcaaca
    16801 tttttgtgct cgatcgtcgt aatggtgaac tggtggttcc ggccccggaa aaaccggttc
    16861 cccaaggtgc tgccaaaggc gattacgtga cccctactca acccttctcg gaactgagct
    16921 tccgtccgac gaaagatttg agcggtgcgg atatgtgggg tgccaccatg tttgaccagt
    16981 tggtgtgccg cgtgatgttc caccagatgc gctatgaagg catttttacg ccgccatctg
    17041 aacagggtac gctggtcttc ccgggtaacc tggggatgtt cgaatggggc gggatttccg
    17101 ttgatccgaa tcgtgaagtg gcgattgcca acccaatggc actgccgttt gtttcgaaac
    17161 tgatcccacg cggtccaggc aacccgatgg aacagccgaa agatgccaaa ggcacgggta
    17221 cggaatccgg catccagcca cagtacggtg taccgtatgg tgtcacgctc aacccgttcc
    17281 tctcaccgtt tggtctgcca tgtaaacagc cagcatgggg ctatatctca gcattggatc
    17341 tgaaaaccaa tgaagtggtg tggaaaaaac gtattggtac gccgcaggac agtatgccat
    17401 tcccgatgcc tgtaccggtg ccgttcaata tgggtatgcc gatgcttggc gggccaatct
    17461 ccacggcggg taacgtgctg tttatcgccg ctacggcaga taactacctg cgcgcttaca
    17521 acatgagcaa cggtgaaaaa ctgtggcagg gtcgtttacc agcgggtggt caggctacgc
    17581 caatgaccta tgaagtgaat ggcaagcagt atgtggtgat ctccgcaggc ggtcacggtt
    17641 catttggtac gaagatgggc gactatattg tggcttatgc gctgccggat gatgtgaagt
    17701 aaccataatg cccggagaga tccgggcata tttccgaata cggtcttttt ataccgtaaa
    17761 ccctaacatc atccccgtat cttcatgctc cagcagatgg cagtgcgcca tataagcatg
    17821 ttctttcggt gcatcgtgat taaacttcac cagcacttcg ctgacattac cttctacctt
    17881 aacggtatct ttccagcctg cgcgatgcgt cgctggcggt ttgccatttt ctgacaagat
    17941 acggaactgc gtaccgtgga tatggaacgg atgcagcatc atgtcgccca cgccagagat
    18001 aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc
    18061 ctgaccgttg attttgttgg catggtggaa atcgaacttc ccgctgtggt tcatatgatt
    18121 catattgccg tgccccatat ggcccatcat ctggctgtga tccatcccgg ccatggcctg
    18181 atcgccatat ttctccatca gcatctgcat ccccatcata tcgagcattg ggtccataga
    18241 aagttgcaga ttgcgtaccg tcagcccttc cagcgaaggt aacgcaggca ggctacttaa
    18301 tgtgtctggc aaagcaccag aggcactaat ggcaatcggc tgaatccgca ttaccggatg
    18361 cggcttgtca aacggcgcaa tcgccatccc catctggctg accggcagcg tcaccaggtc
    18421 aaagggtttg ttatcgttaa cctccaccag cacttcaaaa cgctcgccca tcagcactgg
    18481 cagctcgttc accttcaccg gttcaggtag cagaccgccg tcgctggcaa tcacataaag
    18541 cgggcgattg tcgctggtgg cgaaattgag cgagcgggca ttacagccat tgagcaaacg
    18601 caggcgcagc cagccacgcg gggcagcgtg ttgcgggtag attgcaccgt tggtcagcaa
    18661 cgtatcgcca aaccaaccca cagcggcggt catcacatcc agttgataat caatctgccc
    18721 gtctgcgtta aattttttat cctgaacaat caccgggaca tcatcaatac cccactgttt
    18781 tggcagcatt aatttcagga tctcgtcatc ttcaatcacc accaaaccag ccagccccat
    18841 cgccacctga cgtccggtct tgccatgttg atgtggatgg aaccagcagg tagcggcagg
    18901 ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc ggaataattc cctgcgggcc
    18961 gccgtccact tcacccggta cttccagccc gtgccagtgc aacgtcgtct cttccgtcag
    19021 ttggttgtag atatcaaccg ttaccgcttt gccgcgctgt aatttcaccg ccggccccag
    19081 cagattgccg ttatagcccc aggtagttgc ggttttcccg ccaaaggtgg actgacctgc
    19141 gccaatagtt aactgaatgc gattacgggc atcggtcgtg agcaaatcag ggattggtaa
    19201 cgttgggcgt tccgccgcaa atactgcgcg gctccacagc ggcaaggctg aagccacccc
    19261 cagcgcgacg gaatatttca agaaatcacg acgttgcata gttatttcct tattcttaag
    19321 caggcgaaac aaaatcaaac gttgagcata gtccttcccc ttacgggaag gtcaagccgc
    19381 agacacaatt taatcgacaa taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc
    19441 ttctttaagc ccctggtaga agcaatgaag acgtttttca gaacagtgtt attcggcagc
    19501 ctgatggccg tttgcgcaaa cagttacgcg ctcagcgagt ctgaagccga agatatggcc
    19561 gatttaacgg cagtttttgt ttttctgaag aacgattgtg gttaccagaa cttacctaac
    19621 gggcaaattc gtcgcgcgct ggtctttttc gcccagcaaa accagtggga tctcagtaat
    19681 tacgacacct tcgacatgaa agccctcggt gaagacagct accgcgatct cagtggcatt
    19741 ggcattcccg tcgctaaaaa gtgcaaagcc ctggcccgcg attccttaag cctgcttgcc
    19801 tacgtcaaat aatccctgat acctttttgt agaaatattg accgtgcatc cgcggtcaat
    19861 gttagctatt atgttgcgcc atttttttac gggtgttaac aaaggaggta tcaacccatg
    19921 gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt ttgggcagta ctttgcggta
    19981 gataacgttc tgtatcatga aaagaccgat caccaggatc tgatcatttt tgagaacgcc
    20041 gcatttggtc gcgtaatggc gctggatggc gtagtacaaa ccaccgagcg cgacgagttt
    20101 atctatcatg agatgatgac ccatgttccg ctactggccc acggtcacgc gaaacatgtg
    20161 ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt
    20221 gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctt
    20281 cccaaccata acgccggtag ttacgacgat ccgcgcttta agctggtgat cgacgatggc
    20341 gtcaatttcg ttaatcaaac cagccagacc tttgatgtca ttatctccga ctgcaccgat
    20401 cctatcggtc ccggcgaaag tctgttcact tcggcatttt atgaaggctg caaacgttgc
    20461 ctgaatcctg gcggtatttt cgttgcgcag aacggcgtct gctttttaca gcaggaagag
    20521 gccatcgaca gtcatcgcaa actcagccat tacttcagcg atgttggctt ttatcaggcg
    20581 gcgatcccga cctattacgg cggcatcatg acctttgcat gggcgacaga taacgacgcc
    20641 ttacgccatc tttcaaccga aattattcag gcgcgttttc tcgcttctgg cctgaaatgc
    20701 cgttattaca atccggcagt ccatacggca gcttttgcct taccccagta tctgcaagat
    20761 gcactggctt cacagccgtc ctaaggagaa gataagaaat tgaaaaaact gaaactgcat
    20821 ggctttaata atctgaccaa aagtctgagt ttttgtattt acgatatctg ctacgccaaa
    20881 actaccgaag agcgcgacgg ttatattgct tatatcgatg aactctataa tgccaaccgt
    20941 ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg ccaatattct taacatcgcc
    21001 cgccaggatt acgaaccaca aggtgccagc gtcactattc tggtgagtga agaaccggtc
    21061 gacccgaaac ttatcgacaa aacagaacac cccggcccac tgccagaaac ggtcgttgcc
    21121 catctcgata aaagtcatat ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt
    21181 ttatgtacct tccgcgccga tattgaagtc tctacctgcg gcgtgatttc tccgctgaag
    21241 gcgctgaatt acctgatcca ccagcttgag tccgatattg tgactattga ttatcgcgtg
    21301 cgcggtttta ctcgcgacat taacggtatg aagcacttta tcgaccatga gattaattcg
    21361 attcagaact ttatgtctga cgatatgaag gcgctgtatg acatggtgga tgtgaacgtc
    21421 tatcaggaga atatcttcca taccaagatg ttgcttaaag agttcgacct taagcactac
    21481 atgttccaca ccaaaccgga agatttaacc gacagcgagc gccaggaaat taccgctgcg
    21541 ctgtggaaag aaatgcgcga gatttattac gggcgcaata tgcccgctgt ttaacgtctc
    21601 aggcggagca cggaggctcc gcctgattta cttacttctg ctgcacgaaa ttacggtaag
    21661 ccgccaccac ctgaagaaaa tcctcaacgc cacacagtga caagctctct tcatcgtagt
    21721 agttcatccc ctcttccatt tcatcgccag cgaattccag ttgattggcg cgaaccatca
    21781 cctcttcacc atccatccat agcgtgtatt catgccctgc ccgttgccag gaacgttcgc
    21841 tacctttcaa tgcgtgcgcc gcttgttcca cttcatcaag caaggccagg ttttctttca
    21901 cctcttcatt aaaccagtgc ccgaccactt catgccccat ggacatgcgc acctttacca
    21961 ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg ttcctctgct cccggcaatg
    22021 cgccgaaatc gcattgcgcc attgatgtaa ttatcgcagt cttaccggag aaaaacagcg
    22081 gaacggcgat ggtttagaaa taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc
    22141 gtttatgcgc agcgcgtgcg cttacttatt aaaccgcagt ctggaaaatc accccgtccg
    22201 ctttttcggt gtactgagaa agctggtcga agttcagata acggtaagta tcaacggcag
    22261 ttttatctac ttgcgccacg taggtctggt actcttccgg cgtcggcagt ttgccaatca
    22321 gcgccgcaac agcagccagt tccgcagaag ccaggaagac attcgcgcca gtacccaaac
    22381 ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct
    22441 ggttacccat acacagggaa cagccaggga tctcgatacg agcaccactc ttaccgaaga
    22501 cgctgtagta gccttcttcg gtcagctgcg cagcgtccat acgcgttggc ggtgccaccc
    22561 acaggcgggt cggcaactga cctttgtgcg catccagcag tttaccagca gcacggaagt
    22621 gaccgatgtt agtcatgcaa gaaccgataa atacttcgtc gatcttctcg ccctgtaccg
    22681 cagacagcgg acgtgcgtcg tccgggtcat tcggtgcaca gaggattggc tctttgatat
    22741 ccgccagatc gatgtcgatc actgccgcgt attccgcatc tgcatcggct tccagcagct
    22801 caggattcgc cagccatttt tccatgccct gaatacgacg ctccagggta cgacgatcgc
    22861 cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt agagttcagg tattcaatga
    22921 tcggttcttt gttcagcttg atggtacaac cggcagcaga acgctcggca gacgcatcgg
    22981 tcagctcaaa tgcctgctca actttcagat ccggcagacc ttcaatttcc agaatacggc
    23041 cagagaagat gtttttctta cctttcttct caacggtcag cagaccttgt ttgattgcat
    23101 acagcggaat ggcgtgtacc agatcgcgca gggtgatacc cggctgcatt ttgcctttga
    23161 agcgtaccag aacggattcc ggcatatcca gcggcattac gcctgttgcg gctgcaaatg
    23221 ccaccagacc agaacccgcc gggaaagaga taccgatcgg gaaacgggta tgggagtcac
    23281 caccggtacc gacggtatcc ggcagcagca tacggttcag ccaggagtga atgacgccgt
    23341 cccccggacg cagcgacaca ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg
    23401 tgttcacgtc aactggcttc ggatacgccg cggtgtggca gaaagactgc atcaccaggt
    23461 cagccgagaa gcccaggcac gccaggtctt tcagttcatc acgggtcatc gggccggtgg
    23521 tgtcctggga acctacagaa gtcattttcg gttcacagta cgcacccgga cgaatgcctt
    23581 tcacgccaca ggcacggcct accattttct gcgccagcga gaagccgcga tcgctctcag
    23641 cgacatcttt cgcctgacgg aacacatcac tatgcggcag gccaagtgct tcacgcgctt
    23701 tggtggtcag gccacgcccg ataatcagcg gaatacggcc gccagcacgc acttcatcaa
    23761 tcagcacgtc ggttttcagt tcgaaggttg ccagcagttc gccggtttcg tggttacgca
    23821 cttcaccttt gtacgggtaa acgtcaatta cgtcgcccat gttcaggtta gagacgtcga
    23881 cttcgattgg cagtgcaccc gcgtcttcca tcgtgttaaa gaagattggt gcaattttac
    23941 cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg tggaatatca tcgcccataa
    24001 accacagaac ggagttcgtg gcggatttac gcgatgaccc cgtacccaca acgtcaccga
    24061 cgtacgccag cgggaaacct ttctgttgca gagcttcaat ctgtttgatc gggccaacaa
    24121 caccaggctg atctggctca ataccttcac gggcgttttt cagcatcgcc agcgcgtgca
    24181 gtgggatatc cgggcgtgac cacgcatccg gtgccggaga aaggtcatcg gtgttggttt
    24241 cgccggtcac tttgaagacg gtaacggtca gtttttcagc cagcgccggg cgattcagga
    24301 accattcggc atccgcccag gactgcatta cctgcttcgc atattcgtta cctgctttgg
    24361 ctttctcttc tacgtcatag aagttatcga acatcagcag tgtgtgagaa agtgctttgg
    24421 cagcgatcgg tgccagtttg gcatcatcca gcgcgtcgat cagcggatga atgttgtaac
    24481 caccctgcat agtgcccagc agttcaatgg ctttttccgg agtcagcagt ggggatttgg
    24541 cttcaccttt ggcgacagca gccaggaagc ctgctttgac ataggcggct tcatcgacgc
    24601 cagggggaac acggttggtt aacagatcta acaggaattc ttcttcgccc gcgggcgggt
    24661 ttttcagcag ctctacaagc gcggccattt ggtttgcatc caggggtttg ggcgcaatcc
    24721 cctcagcggc acgctcagct acgtgcttac ggtattcttc tagcacgacg gttctcctcg
    24781 ctctcattgt catagtgcgg caggcgattc tcttcacgct cctgtgagac agcagtttgt
    24841 agggtaaatg cccagtaccg caacgggcag aatagcagga ttttgacgat gtgttaatct
    24901 gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta aggatggtct agagattatt
    24961 tccctccgta aaaacttcgt taattcgcat ggtgataatc acctttctca acgaatccca
    25021 agattagtca aaatttaaac tacggcctct ttatactcgg attcacagca cctgcgggtg
    25081 gcagttcgcc gaccattgcg atttccttga gatccgaatt atgaaaatga ctttgccgtt
    25141 taaaccccat gtgctggcgc taatttgcag tgccgggctc tgtgccgcct ctgccgggct
    25201 atatataaaa agccgcacag tggaagcgcc tgtggaagcg caatcgacac aacagactgc
    25261 gcctgacatc tccgcagtta cgcttccagc aacggtttcc gtgcctcctg taacacctgc
    25321 cgtcgtcaaa tccgcattca gcactgctca aatagatcaa tgggttgcac ctgtcgcgct
    25381 ataccccgac tctctgcttt cacaagtgtt aatggcatca acctatccgg caaacgttgc
    25441 tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg ctattcaggc
    25501 ggtatctgac cagccctggg acgccagcgt caaatcactg gtggcctttc cacagttgat
    25561 ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca
    25621 gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac aaaccggctc
    25681 gctgaagtca tcaactgaac aaaagatcat cacgacaacg aaaaaagtcg taccggtaaa
    25741 tcagcccgcc aacgcccctg ccacacaatc aaataccgtt tctacgtcca gccccgtcgt
    25801 cgcagaacct gcaccgaccg taataaccat tgagccagcc aatccggatg tggtttatat
    25861 tccgaactac aacccaaacg tggtttacgg cagttgggcc aataccgctt atccaccagt
    25921 ttatctgccg ccgccagccg gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta
    25981 tagcatgggc gttgctacca catacgcact attcagcagc atcgactggg acgacgacga
    26041 tcatgaccat gaccatcatc atcatgacga tgatgattat catcaccacg atggcggtca
    26101 tcgtgacggt aatgactggc aacacaacgg cgacaacatc aatatcgacg tcaacaattt
    26161 caaccgtatc accggtgagc atcttactga taagaatatg gcatggcggc acaatccaaa
    26221 ctaccgtaat ggtgtgacct atcatgatca ggatatggca aagcggtttc atcaaaccga
    26281 tgtcaacggc ggaatgagcg ccacgcagtt acctgctcta tcgcgcgaca gccagcgtca
    26341 ggcggcagca agtcagtttc agcaacgaac acacgccgcc cccgtcatta cgcgagatac
    26401 ccaacgtcag gcagcggcac agcggtttaa tgaagcggaa cactatggca attatgacga
    26461 cttccgcgag ttcagccgcc accaaccact gacccagcag caaaaggacg ccgctcgcca
    26521 gcgttatcag tctgcctcac ctgagcagcg ccaggcggcc cgtgagaaaa tgcagactag
    26581 cccgcagaat caacagcaaa gagatgcagc acgtcagcgt attcagtccg cgtcgcctga
    26641 gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaag agcaacgtga
    26701 taacgcccgc cagcgtgttc aatcggcatc acctgaacaa cgtccgtttt ttcgggagaa
    26761 agttcaggag agccgcccgc aacgtctaaa cgacagtaac cgtactgcca ggctgaataa
    26821 cgagcagcgg tcagcagtac gcgaacgtct ctctgaacgt ggggcaagaa gactggaaag
    26881 gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg ccggatgttc
    26941 cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt aatgctacct
    27001 tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc atggatggtc
    27061 agtgcgatgt cttcagcgtc acaacccatt tcgattgcca agccgatttc acccagcaac
    27121 tcgccgccgt tagtgccgac aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa
    27181 atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga agcagcccac
    27241 gggaaggtgg cggtttcgta gctgatgcct ttctctttcg cttctttctc agtcagacct
    27301 acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtaa
    27361 tgttttttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc
    27421 agcattggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt ggtacgcagc
    27481 tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt gcctgcgtcg
    27541 aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt
    27601 tcagcgggtg cttttttgcc ttccatcgtc acataaatgc cgtcttcttt cgcttcaacg
    27661 gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt ggtaaagact
    27721 ttaacgatgt ctttatcagc tgccgggatg acctggtcga acatttcaac cacgtcaatc
    27781 tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc gccacccatt
    27841 accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc ccagatacgc
    27901 ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc aatgatcgcg
    27961 ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt gttagccccg
    28021 gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga
    28081 ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc gatatcggtt
    28141 ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt
    28201 gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg
    28261 gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg
    28321 gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc
    28381 atgacctcta tatatttatc tccggcgggt ctgacgtttt ttttatgacg cccattatcc
    28441 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    28501 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    28561 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    28621 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    28681 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    28741 atcgcggact tggaaacgcc gaggatagcc acttccggcg cattcacaat cggcgcgaag
    28801 tgggtagtac ccaggccgcc gatgctcgag atggtgaagc aaccgccctg catttcgccc
    28861 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    28921 atgatgcctt tcttgttgac gtctttgaat accgggacaa ccagaccgtt cggggtatcc
    28981 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    29041 gaactgttga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    29101 accggggtga tcttcacatc cagcttacgt ttcgctgctt cttcgttctg ctgtttacgg
    29161 aacgcttcca gctcagtgat atcggttttg tcgaagtgag taacgtgcgg gatcatcacc
    29221 cagttacggc tcaggttagc accagagatt ttctgaatgc ggcccagttc cacttcttcg
    29281 atttcaccaa acttgctgaa gtccacttta ggccacggca gcatgcctgg gataccgccg
    29341 ccagttgccg ccggagctgc ttctgcacgt ttgattgctt ctttcacgta agcctgaacg
    29401 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    29461 aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa cgtaagcgtc gttttcagca
    29521 aattcagatt tgccttcggc tttcgcagcc ggtgctgctg ccggggcttc agcttttgct
    29581 gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggcgc tgcgccttca
    29641 acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt gactttcagt
    29701 tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg
    29761 gtgatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact
    29821 tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc tgctggtgca
    29881 gaggccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc agcttcaccc
    29941 gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg
    30001 atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt gtcgccttct
    30061 acggtgatca gcgactgttc agcttcaact ttgtcgccca ctttgaccag gatttcggtc
    30121 acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc tgcagccgct
    30181 ggtgctgctg ccggagcggc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct
    30241 gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca
    30301 gagactttga tctctttaac gatacccgcc tgcggagatg gtacttccat agaggctttg
    30361 tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg
    30421 atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt
    30481 attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg
    30541 cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg
    30601 ccgcaaccac gacataagaa gcatcaactt caaagtggtg acgcaggttc tcacggctgt
    30661 cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt acgtaagtac
    30721 ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccgga gcgtcgttca
    30781 tcacctgagc gatatacgga acgcgcggag tttccagcgg gtgcagcatg ttccagcgtt
    30841 cacaatcctg accatcacgc gccagttcgg tgaaggaagt cacgctataa acgtcagaac
    30901 ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatagaac
    30961 cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac
    31021 ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca
    31081 gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt
    31141 catgcatgat gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggga
    31201 tagtcagcga ctgaatatgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg
    31261 tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg ccagccgccc
    31321 agcacagatc gccgatacgc tggaaaccga acatcgagta atagatgtag aacgggatca
    31381 tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacaa cctgcgccca
    31441 gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct
    31501 gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga
    31561 acagaccttc cataccgaag gtacgcgctt cgtcggcgat gatcggtacc agacgatctt
    31621 tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata gtggtagaga
    31681 tctctttgct ctgctcttcc agcagcgcgc cgaaatcttg caggctcggc agctccagct
    31741 tctcggtgaa gttcggctgg cggcttggca gataaccgtg cagtttctga cgttgagcgt
    31801 gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat
    31861 ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca
    31921 ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt
    31981 taatggtatg agcaaggatt accgtcgctt tgcctttggt ttcctgcgct ttcttgaatg
    32041 cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag
    32101 tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcgcgaacat
    32161 acgcaccatc tttcgatttg aaggtctggt aatcgccgtc aacggtttcg ttcatcagct
    32221 ggatcagttt accgctggta tctttacgca gcagttcgtc ccaacggcta ccccacatca
    32281 ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc
    32341 cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg aagaccagat
    32401 tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt
    32461 caccgtcgcc gaggaacgcg taaacggttt gtttagaggt atctttcagg ccacggtgtt
    32521 ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccagc cccatagata
    32581 cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat
    32641 tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttccagga
    32701 aagcacgagc gtaaacgcct ggggagatgt ggccctggaa gtaaaccagg tcgccgccat
    32761 cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag
    32821 actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg
    32881 tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg
    32941 gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata ccggtgcctg
    33001 cagctacgtt gactccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag
    33061 cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt
    33121 cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat
    33181 aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc aataagacag
    33241 gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct tctggataat
    33301 ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg
    33361 cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc
    33421 gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg
    33481 tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg
    33541 acattctgag ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg
    33601 gccgcttcgg tgacggcaat ctgatactgg agtacggcat ttgattccgc gtccagatcg
    33661 ccagactgct gcgccagctc tatggcatgg tggagttcac ggatgcgttc cttgtcttca
    33721 tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg
    33781 agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag
    33841 ctttgccaga ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc
    33901 ttcgcttcga ggcgttgaat cgcctcacgc aaagagggac gggagacgtc gaactgtttt
    33961 gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa
    34021 aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc
    34081 atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt
    34141 gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa
    34201 actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa
    34261 tgttaaattt acccatcaag gggtgacctt aacagaattg aaacgaaaaa atatttcctc
    34321 tgaaccgatt caccaagcaa caaaacgcat aacaattcaa ttatgtgaat taatatgtaa
    34381 aatgaatgtt cagggaagga gagaaaggta gtatttacct atgttttctt tttacattgc
    34441 ttattatcac tgcaatcgcc accataacct tctcgtagcg aaaatcatca cttaaccaga
    34501 acgtgatgat tttcaggtga cagattctgt ttagagttca ttctgaatct ttgaacaaag
    34561 gcaatccttg cgaacgccgc cattctttga cgattttcag gatgttttca ggttcatcat
    34621 ctcccttctt tgcaggatag tagataagat cgctcatcaa cggatgttgt gtaattctgg
    34681 taagatgttc ttctagatca tcccaatatt tgtcaagc
//
LOCUS       DAJOTB010000034        33870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJOTB010000034 DAJOTB010000000
VERSION     DAJOTB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.034"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1969
                     /gene="betT"
                     /locus_tag="OHN28_001675"
     CDS             <1..1969
                     /gene="betT"
                     /locus_tag="OHN28_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCQ2297083.1"
                     /translation="GLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLLAATLYI
                     VFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQP
                     PEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYPIF
                     GKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL
                     SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNR
                     FMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLI
                     IPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLAQYPAFTFSASVAT
                     ITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGIS
                     ALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKR
                     LSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRGAYVELKNLPPEEGQQLGHLDLL
                     VHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQV
                     ITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2847..3935
                     /gene="pdeL"
                     /locus_tag="OHN28_001676"
     CDS             2847..3935
                     /gene="pdeL"
                     /locus_tag="OHN28_001676"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HCQ2297084.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(3977..4909)
                     /locus_tag="OHN28_001677"
     CDS             complement(3977..4909)
                     /locus_tag="OHN28_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2297085.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHRES"
     gene            complement(5001..5498)
                     /locus_tag="OHN28_001678"
     CDS             complement(5001..5498)
                     /locus_tag="OHN28_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2297086.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            5756..6361
                     /locus_tag="OHN28_001679"
     CDS             5756..6361
                     /locus_tag="OHN28_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCQ2297087.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            6401..7264
                     /locus_tag="OHN28_001680"
     CDS             6401..7264
                     /locus_tag="OHN28_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2297088.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            7254..8801
                     /gene="fdrA"
                     /locus_tag="OHN28_001681"
     CDS             7254..8801
                     /gene="fdrA"
                     /locus_tag="OHN28_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2297089.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            8801..10219
                     /locus_tag="OHN28_001682"
     CDS             8801..10219
                     /locus_tag="OHN28_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2297090.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            10372..11322
                     /locus_tag="OHN28_001683"
     CDS             10372..11322
                     /locus_tag="OHN28_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCQ2297091.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQDGKEVIITTPECLPAALRGETGTHIIKT"
     gene            11332..12714
                     /locus_tag="OHN28_001684"
     CDS             11332..12714
                     /locus_tag="OHN28_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCQ2297092.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            13091..14140
                     /gene="yahK"
                     /locus_tag="OHN28_001685"
     CDS             13091..14140
                     /gene="yahK"
                     /locus_tag="OHN28_001685"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCQ2297093.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            14763..15007
                     /locus_tag="OHN28_001686"
                     /pseudo
     CDS             14763..15007
                     /locus_tag="OHN28_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15024..15695)
                     /locus_tag="OHN28_001687"
     CDS             complement(15024..15695)
                     /locus_tag="OHN28_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCQ2297094.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            15842..16117
                     /locus_tag="OHN28_001688"
     CDS             15842..16117
                     /locus_tag="OHN28_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2297095.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(16218..17804)
                     /gene="prpR"
                     /locus_tag="OHN28_001689"
     CDS             complement(16218..17804)
                     /gene="prpR"
                     /locus_tag="OHN28_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HCQ2297096.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            18043..18933
                     /gene="prpB"
                     /locus_tag="OHN28_001690"
     CDS             18043..18933
                     /gene="prpB"
                     /locus_tag="OHN28_001690"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HCQ2297097.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            19093..20262
                     /gene="prpC"
                     /locus_tag="OHN28_001691"
     CDS             19093..20262
                     /gene="prpC"
                     /locus_tag="OHN28_001691"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HCQ2297098.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            20296..21747
                     /gene="prpD"
                     /locus_tag="OHN28_001692"
     CDS             20296..21747
                     /gene="prpD"
                     /locus_tag="OHN28_001692"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HCQ2297099.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            21787..23673
                     /gene="prpE"
                     /locus_tag="OHN28_001693"
     CDS             21787..23673
                     /gene="prpE"
                     /locus_tag="OHN28_001693"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HCQ2297100.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            23998..25257
                     /gene="codB"
                     /locus_tag="OHN28_001694"
     CDS             23998..25257
                     /gene="codB"
                     /locus_tag="OHN28_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2297101.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            25247..26530
                     /gene="codA"
                     /locus_tag="OHN28_001695"
     CDS             25247..26530
                     /gene="codA"
                     /locus_tag="OHN28_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HCQ2297102.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(26651..27262)
                     /gene="lacA"
                     /locus_tag="OHN28_001696"
     CDS             complement(26651..27262)
                     /gene="lacA"
                     /locus_tag="OHN28_001696"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HCQ2297103.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGCHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(27328..28581)
                     /gene="lacY"
                     /locus_tag="OHN28_001697"
     CDS             complement(27328..28581)
                     /gene="lacY"
                     /locus_tag="OHN28_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HCQ2297104.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(28633..31707)
                     /gene="lacZ"
                     /locus_tag="OHN28_001698"
     CDS             complement(28633..31707)
                     /gene="lacZ"
                     /locus_tag="OHN28_001698"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCQ2297105.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(31830..32912)
                     /gene="lacI"
                     /locus_tag="OHN28_001699"
     CDS             complement(31830..32912)
                     /gene="lacI"
                     /locus_tag="OHN28_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCQ2297106.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(32989..33822)
                     /gene="mhpR"
                     /locus_tag="OHN28_001700"
     CDS             complement(32989..33822)
                     /gene="mhpR"
                     /locus_tag="OHN28_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HCQ2297107.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLLEEGYVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
BASE COUNT         8178 a         8642 c         9078 g         7972 t
ORIGIN      
        1 cggactgatt ttgttgtttt ccctgacaac gatcctgttt cgcgacttct cggccctgtg
       61 gattggccgc acgctggact gggtttctaa aaccttcggt tggtactatc tgctggcggc
      121 aacgctctat attgtctttg tggtctgtat cgcttgttcg cgttttggtt cggtgaagct
      181 cgggccagaa caatcaaaac cggaattcag tttgctgagt tgggcggcga tgctgtttgc
      241 tgccgggatc ggtatcgacc tgatgttctt ctccgtagcc gaaccggtaa cgcagtatat
      301 gcagccgccg gaaggcgcgg gacagacgat tgaggccgcg cgtcaggcga tggtctggac
      361 gctgtttcac tacggcttaa ccggctggtc gatgtatgcg ctgatgggca tggcgctcgg
      421 atactttagc tatcgttata atttgccgct caccatccgc tcggcgctgt acccgatctt
      481 cggtaaacgg attaacgggc cgataggtca ctcagtggat attgctgcgg tgatcggcac
      541 catcttcggt attgccacta cgctcggtat cggtgtggtg cagcttaact atggcttgag
      601 cgtgctgttt gatattcccg attcgatggc ggcgaaagcg gcactgatcg ccttgtcggt
      661 gatcatcgcc actatctcgg tgacatccgg tgtcgataag ggcattcgcg tgttatcgga
      721 actcaacgtt gcgctggcgc tgggattgat tctgttcgtg ttgtttatgg gcgacacctc
      781 gttcctgctt aatgcactgg tgctgaacgt tggcgactat gtgaatcgct ttatgggcat
      841 gacgctcaat agttttgcct tcgaccgtcc ggttgagtgg atgaataact ggacgctctt
      901 cttctgggca tggtgggtgg catggtcgcc gtttgtcggc ttgttcctgg cgcgtatctc
      961 gcgtgggcgt accattcgcc agttcgtgct gggcacgttg attattccgt ttaccttcac
     1021 gctgttatgg ctctcggtat tcggcaatag cgcgctgtat gaaatcatcc acggcggcgc
     1081 ggcatttgcc gaggaagcga tggtccatcc ggagcgcggc ttctacagcc tgctggcgca
     1141 gtatccggcg tttaccttta gcgcctccgt cgccaccatt actggcctgc tattttatgt
     1201 gacctcggcg gactccggtg cgctggtgct ggggaatttc acctcgcagc ttaaagatat
     1261 caacagcgac gcacccggct ggctgcgcgt cttctggtcg gtggcgattg gcctgctgac
     1321 gctaggcatg ctgatgacca acgggatatc cgcgctgcaa aacaccacgg tgattatggg
     1381 cctgccgttc agctttgtga tcttcttcgt gatggcgggg ttgtataaat ctctgaaggt
     1441 agaagattac cgccgtgaaa gtgccaaccg cgataccgca ccgcgaccgc tggggcttca
     1501 ggatcgcctg agctggaaaa aacgtctctc gcgcctgatg aattatccgg gcacgcgtta
     1561 cactaaacag atgatggaga cggtctgtta tccggcaatg gaagaagtgg cgcaggaact
     1621 gcggttgcgc ggcgcgtacg tggagctaaa aaacctgccg ccggaagagg gacaacagtt
     1681 ggggcatctg gatttgttgg tgcatatggg cgaagagcaa aactttgtct atcagatttg
     1741 gccacagcaa tattcggtgc cgggctttac ctaccgcgca cgtagcggga aatcgaccta
     1801 ctaccggctg gaaaccttcc tgttagaagg cagccagggc aacgacctga tggactacag
     1861 caaagagcag gtgatcaccg atattctcga ccagtacgag cggcacctta actttatcca
     1921 tctccatcgt gaagcgccgg gccatagcgt gatgttcccg gacgcgtgat tgttattgca
     1981 taaaaccgcg ccatgtctgc atatggcgcg gtttctctta cctcttgata cgttatatct
     2041 gtgtacagtt aagcccttag tatcgattga tgattgccag gcaacagata ataaaaaaga
     2101 aaagaactat tgaagcccaa aacctacgct tggactgttg tgaatgttca ataagcttag
     2161 tcttatttaa tgtaaatatt ggtgctaatt tgaaatgacg ctttattcat gggaaacgcg
     2221 tagcgcaaat caaccaaaat caatgtaacc gtttgttgtt taaggtttaa ttgtctgtgt
     2281 ctgatatttt attgattgtt ttaaatattg tttttattgt cattgttata atgttggcta
     2341 tctattgctg aatggattca gtcttaaaaa gcaggttgtt aagggattag catagagtaa
     2401 tgacgtgtat gcataaccaa catctttact cattatgtca ttgaatgttg acgctatgtg
     2461 tttatgaggt tgaggtattt tcagttgatc tggattgtta aattcatata atgcgccttt
     2521 actcatgaat ggacgtcaat acgtagtggg aaattataaa tatcgaaata gtccaactac
     2581 ttctttatta ccaaaaatga gtatctgaat tttaatattg cattcttgcg tgattatctc
     2641 ctgagtttga cttgtgatta cctttttaag gtatttagcg taactgtttt tgagcgagca
     2701 tcagaggtaa agataatctt cttgatagtg atgtgggatg ttatacgtat ggcatcgcta
     2761 atgtttatgg ttacccctta tgtgtgctca ggaatccaca ggtaatcact catactgaac
     2821 agcgataaaa gataaaaggt gtgttcatga attcatgtga ttttcgtgtt tttctgcaag
     2881 agttcggtac aacggttcat ttgtcattgc ctggtagcgt atccgagaaa gaacgactgc
     2941 tactcaagct gctgatgcag ggaatgtctg taacagaaat atcacagtac agaaatcgca
     3001 gtgcaaagac catttcacat caaaagaaac agctctttga gaaactgggg attcagagcg
     3061 atattacttt ctggcgggat attttctttc agtacaatcc ggagatcata tccgccacgg
     3121 gtaataatag tcacaaatat attaatgata atcactatca ccatatcgtc acgcctgaag
     3181 ccatcagtct ggcgttggaa aaccacgagt ttaaaccgtg gatccaaccg gttttctgcg
     3241 cgcagactgg ggtactgacg ggctgtgaag tgcttgtccg ctgggaacat ccacaaacgg
     3301 gaatgatccc accggatcag tttattcctc tggcggagtc atccggtctt attgtcataa
     3361 tgactcgcca gctgatgaaa cagactgcgg atattctgat gccggtaaaa catttgctgc
     3421 cggacaattt ccatattggt atcaacgtct cggcgggctg ttttttggct gcgggatttg
     3481 aaaaagagtg tctgaacctg gttaagaaat taggtaacga taaaatcaag ctggttcttg
     3541 agttgactga acgtaatcct attccggtaa cgccagaagc cagagcgata tttgacagcc
     3601 ttcatcagca caatattact tttgcgctgg atgactttgg tacgggttat gcgacctatc
     3661 gttacttgca ggcgttcccg gtcgatttta ttaaaattga caagtcgttt gtgcaaatgg
     3721 cgagtgtcga cgaaatctcc ggtcatattg tggacaatat tgtcgaactg gcgcgtaagc
     3781 ctggtttgag tatcgtggcg gaaggggtag aaacccagga gcaggcggat ttaatgatcg
     3841 gcaaaggcgt tcacttttta cagggctatt tgtactctcc gccagtaccg ggtaataaat
     3901 ttatctctga atgggtaatg aaagcaggtg gttgatgtaa accgctattc acagcgcatc
     3961 gggaggttgg cagcgattaa ctctcccgat gcagtaaatc ctgatagatc cccgttaata
     4021 tgccatttgg cgcaaattgt tttttaatcc actgcgtcac ctgaccggtg gcagagtgct
     4081 gggttgcgag taacatgcgt gaatcctggc gcgggttatg gatttgtcgg gtaaccagca
     4141 aggattgcgc catcgcctca cggaccatgt aatccggcaa aaaaccaatt ccttcaccta
     4201 aaatctggca ctgacatttg gtgttgaaat cgggcaccag aatcgattcc tgcccatgca
     4261 gcaaccagcc tactttttta ttaatcgtat gcgcggtgtc ctccaccatg atattagggt
     4321 agagacgcag ctggctttcg gcgatcggct ccggcacaaa ggctaatgga tgatccggag
     4381 cgatggcaaa agcccagcga atcgcgccaa tctccgtgta atcaatgccg ccaccgtcca
     4441 gcagcgtgtc gggtgcgccg atagcgatat ttgcctgatt attaataatc gcatcccaga
     4501 cgccgttata cacttcggtg gtgacggtaa tctggcaggt aggaaactgc tttttcagca
     4561 cctgtaataa gcgtgcggtg tgtttgggcg tataaagcag ctgattaata caaatgcgca
     4621 cacgcgcttc tatgccctgc gaaatagtat cgatactgcg tttaatggcg tgaaaatcat
     4681 tcagcaggtc ggtagctttg cggaaaaaat aacgcccgga ctccgttaac tcaatgctgc
     4741 gggtactgcg agtgaacagc accacatcca gccctgtttc cattcgcttg atggtgtagc
     4801 taatggcgga agtggttaaa cccaactctt ctgcggcttt actgaagctg ccaaaacgtg
     4861 cagcggtagt aaatgccaac agattctctt cggtaaaaat tgagttcatt aaaccattcc
     4921 cgccattatc gaatatgaat ttctccagcc atgactgtag aggtatcggt taaagataga
     4981 taatcatttt tgaataactt ttaatacccg tcgcgtttag tcgtcgcttc gcttaatttt
     5041 tgtcctccat attctgaaat tatccccacg cagttacccg ccagaaaacc cagtaatgtc
     5101 accgtccagt tcatcccgct ggcaaaaaat agcgtcatgc cgagaaagcc tccgggaata
     5161 aaagacaata aaccgaaacg tccttgccag accactaccg ctgagaaagg cacggtagta
     5221 atcacgctcg cccagaaccc agaaatccca ctcaccgagg ccagccaacc ggcagccagc
     5281 gcggcgaaaa atgcccacac catcccggaa taattaaccg ccagacttct gataaacccc
     5341 gatttaccgc cgcctgcggc ataaaaactg caaaaggcga cgaagccaat ggttcctaat
     5401 aattcccatc cctgagagag cccgacatgt gaagacacta actgccaaag ccctgcacaa
     5461 ataccgacgg tgacacccgt tgccgtgagt ccgttcatct tgttcccctc tggttagaca
     5521 tggacaaaag ctactcttac atgtgcagac tatagagaaa taaaacctga ttgagtgaaa
     5581 gggtatcggg tcaaagaaac aaatattgca ctaccgcaca ctgcgaaaag attgttgaac
     5641 atcattcaac aaaaaggcga aatacggcga attaaattta agcgggttct tttatcagat
     5701 tatcgttgct attctcaatt catcgaaaac agccacaaaa accaggggaa tgtgaatgac
     5761 tataaaaaat ctacctgccg actatttatt ggctgcgcaa cagggtgata tcgataaagt
     5821 aaaaacctgc cttgcgctgg gtgtcgatat taatacctgc gatcgtcagg ggaaaacggc
     5881 aattacgctg gcaagtttat atcagcaata tgcttgcgtt caggcattaa ttgatgccgg
     5941 agcggatatt aataagcaag atcatacctg tttaaatcct tttttaatta gctgtctgaa
     6001 cgatgattta acgctactac gaattatttt accggctaaa cccgatctta attgcgtaac
     6061 ccgttttggc ggtgtcggcc tgacgcctgc ctgtgaaaaa ggccatttaa gtattgtaaa
     6121 agagcttttg gcaaatacgg agattaacgt taaccagact aaccatgttg gctggacacc
     6181 gttgctggaa gcgattgtgc ttaatgatgg tgggattaaa cagcaggcga ttgtccagtt
     6241 attgctggaa cacggtgcca gcccgcatct gaccgataaa tatggcaaaa cgccgctgaa
     6301 actggcgcgg gaacggggct ttgaagagat tgcgcagttg ctgatcgccg caggtgcata
     6361 aaccgggagg cttgttatca acacaccaga aaaacggtgt gtgtgggcgc taactgcgga
     6421 tgctgatttt ctggcgcagc gggggcaagg acaggttgaa caggtctttg ccagagcggt
     6481 aaatatcgca ctcccggctc gccagcagtt gctgacgctg ctttgtgaag agtacgacaa
     6541 tgcgcctaac agttgtcggt tggcactcac tcactttaat ggtctgttcc ggcatgatga
     6601 taaggttcag tttgacgatc aaggcattac gattggtcaa catcatcata tagagatgag
     6661 tcattgccag cgttggctgt ccccaacctt gcaaatgacc gctgtgaatt ttcaccttat
     6721 cgcctggcaa cagtggtacg acattattca tcagcacctg ggggaaaatg aaaccctgtt
     6781 taattatcgc ggcgataatc cgttttatca ggcgttaaat aaagaactac atattaaacg
     6841 acgggcagtt attcaggccg taaacgataa acaaaatatc gcctcagcag tcgccagtat
     6901 gatggggtta gggattggcc ttacgccatc agccgatgat tatctgaccg gactggtact
     6961 tattttattt atttccggac atccggcgga aaaatacaaa gaggaatttt atctcggcct
     7021 gcaacgcggc agaaataata ccacgttatt aagtgccatc acgctggaag ccgcattaca
     7081 acaacgctgc cgggaaaata ttcatcgttt tattcacaac attatttatg acatccctgg
     7141 gaacgcaact caggcaatag aaaaaattaa acatattggc tccagttccg gctgcgacat
     7201 gctgtatggc atggccgatg gttgtgcgct gagtcaaacc tacggaggga attatgtcag
     7261 ttaaaatagt cattaaaccg aatacctatt ttgattctgt ctcgctgatg tctatctcca
     7321 cgcgtgcaaa taaactcgac ggcgtcgagc aggcatttgt ggcgatggcg accgaaatga
     7381 acaaaggcgt gttgaagaat ttaggactgc tgacgccgga gctggagcag gcgaaaaacg
     7441 gcgacctgat gattgtcatc aatggtaaat cgggtgtgga caacgagcag ttgctggcgg
     7501 agattgaaga actgttcaac accaaagcgc aaagcggctc gcacgaggcg cgttacggca
     7561 ctattgccag cgccaaaaag catatcccgg aaagtaacct ggcagtgatt tcggtcaacg
     7621 gtctgtttgc cgctcgcgaa gcgcgtcagg cgctgcaaaa tgacctcaac gtgatgctgt
     7681 tttccgataa tgtctcagtt gaagatgaac tggcgctcaa acagttggcc catgaaaaag
     7741 ggctgctgat gatggggcca gactgcggca cggcgattat caacggcgcg gcgctctgct
     7801 ttggtaacgc cgtgcgtcgc ggcaacatcg gtattgttgg cgcatctggc accggcagcc
     7861 aggaattgag cgtccgtatc catgaatttg gtggcggcgt ttcacaactg atcggcaccg
     7921 gcgggcgcga cctgagcgag aaaatcggcg gactgatgat gctcgatgcc atcagcatgc
     7981 tggaaaacga tccgcaaact gaaatcattg tgcttatctc caaaccgcct gcgcctgcgg
     8041 tggcccgcaa agtgctggaa cgcgcacgcg cctgccgcaa gccagtggtg gcctgcttcc
     8101 tcggtcgtgg cgaaacgcca gtggatgagc aggggctaca gtttgctcgc ggcagcaaag
     8161 aggcggcatt gaaagcggtg atgctctccg gcgtgaaaca agaacatctc gatctgcata
     8221 cgcttgacca gccgttgatt gcggatgtgc gcgcgcgtct gcaaccgcag cagaaataca
     8281 ttcgtggtct gttctgcggc ggtacgctgt gtgacgaaac cctgttcgcg gtgatggaaa
     8341 aacatggcga tgtctacagc aatatccagc ccgatcctga attccgcctg caagatatca
     8401 accgcagcat caaacacacc ttcctcgact ttggcgatga tgacttcacc aacggcaagc
     8461 cgcatccaat gattgacccg accaaccgta tcagccgctt gatcgaagaa gcgcgcgatc
     8521 cagaagtggc ggtgatcgtg atggattttg tgctcggatt tggatcgcat gaagatccgg
     8581 tcggttccac catcgaggcg atcaaagaag cgaaagcgat cgccgctgcc gaaggacgcg
     8641 agttgatcat tctcgcctat gtgctgggca ccgatctcga tacgccatca ttagaacaac
     8701 aaagccagat gctgcttgat gccggagtga ttctggcgag cagcagcacc aataccggat
     8761 tgctggcgcg tgaatttatc tgcaaagggg aggaagcctg atgagccagt cactgtttag
     8821 ccaaccgttg aacgttatta acgtcggtat cgccatgttt agcgatgatc tgaaaaagca
     8881 gcatgtagaa gtgacccaac tcgactggac gccgccgggg cagggcaata tgcaggtggt
     8941 gcaggcactg gacaacattg ccgattcgcc gctggcggac aaaatcaccg ccgctaacca
     9001 gcaggcgctg gagcgcatta tccagtcgca tccagtgctg attggttttg atcaggcgat
     9061 taacgtggtg ccgggcatga cggcgaaaac cattcttcac gccgggccgc cggtcacctg
     9121 ggaaaaaatg tgcggcgcga tgaaaggcgc ggtcaccgga gcgctggtgt ttgaaggact
     9181 ggcgaaagat ctcgacgagg cggctgagct ggcggcttcc ggggagatca ccttctcgcc
     9241 gtgccacgag cacgactgtg tgggatcgat ggcgggcgtc acctcggcct cgatgtttat
     9301 gcacatcgtg aaaaacaaaa cctatggcaa catcgcttat accaacatga gcgagcagat
     9361 ggcgaagatt ttgcgtatgg gcgctaacga ccagagcgtg atcgaccgcc tcaactggat
     9421 gcgcgatgtg caggggccaa tgctgcgtga cgcgatgaaa attatcggcg agatcgatct
     9481 gcgcttaatg ctggcgcagg cgctgcatat gggcgatgaa tgccataacc gcaataacgc
     9541 cgggacgacg ctgctgattc aggcgctgac gccggggatt attcaggcgg gttattccgt
     9601 tgagcaacag cgcgaagtgt ttgagtttgt cgccagcagc gattacttct ccggcccaac
     9661 gtggatggcg atgtgtaaag cggcgatgga tgcggcgcac ggcattgaat acagcaccgt
     9721 ggtgaccacc atggcgcgta acggcgtcga gttcggcctg cgggtcagcg gcctgccggg
     9781 gcaatggttt accggcccgg cgcagcaggt catcggcccg atgtttgccg gttataagcc
     9841 ggaagattcg gggctggata tcggcgacag cgccatcacc gaaacctacg gtatcggcgg
     9901 atttgcgatg gcgaccgcgc ccgcgatcgt tgcgctggtg ggcggcacgg tggaagaggc
     9961 tatcgatttc tcccgtcaga tgcgtgaaat cacccttggc gaaaacccca acgtcaccat
    10021 tccgctgctc ggttttatgg gcgtgccgtc ggcaatcgac atcacccgcg tgggtagcag
    10081 cggcattctg ccggtgatca acaccgccat tgcccataaa gatgcgggcg tcggcatgat
    10141 tggcgcgggc attgtgcatc cacccttcgc ctgcttcgag aaagccattc ttggctggtg
    10201 cgaacgttac ggcgtctgac ctgcaaagta cgcgttgtct ctgccggatg cggcgtgaac
    10261 gccttatccg gcctacgagt ggcacgagaa ttgtaggcct gataagcgta gcgcatcagg
    10321 cagtctggcg ttggtcataa ccccatcacc ctctgtcgcg gacataacaa catgaaagaa
    10381 cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag ccagtcgatt
    10441 gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat gctggcttcc
    10501 gattacgaca ttgtgctgac ccacggcaac gggccgcagg tcgggctgga tttacgccgt
    10561 gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa ctgtgtggcg
    10621 gatacgcaag gtggcattgg ctatctgatc cagcaggcgc tgaacaaccg actggcgcgt
    10681 cacggcgaga aaaaagccgt cactgtggtg actcaggtgg aagtggataa aaacgatccg
    10741 ggttttgccc atcccaccaa gcccatcggc gcattcttta gtgaaagcca gcgtgacgaa
    10801 ctacaaaagg caaaccctga ctggcgtttt gttgaagatg ccggacgggg ctatcgccgc
    10861 gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ctgccattaa ggcgctgatc
    10921 caacaaggct ttgtggtgat tggcgcgggc ggcggtggaa ttcctgtggt gcgcaccgaa
    10981 gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctccac cgcgctactg
    11041 gcccgcgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa agtctgtatc
    11101 cactttggca agccgcagca acaggcgctc gatcgcgtag atattgccac catgacccgc
    11161 tatatgcagg aagggcattt cccacctggc agcatgttgc caaaaatcat cgccagcctg
    11221 acgttcctgg aacaggacgg caaagaagtg attatcacca cgccggaatg cctgcctgcg
    11281 gcgctacgcg gcgaaacggg cacccatatt attaaaacct aaggacgtaa gatgaaagaa
    11341 aacaatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc cgccgcgctg
    11401 tttggtacct cagtgccgct cgcccatgcg ggggtagctg gcaccctaaa ctgcgaagcg
    11461 aacaacacca tgaaaatcac tgacccgcat tactatctcg ataacgtgct gctggaaacc
    11521 ggttttgact acgaaaatgg cgtggcggta cagacccgca cggcgcgcca gaccgtggag
    11581 attcaggacg gtaaaattgt tgccctgcgc gagaacaagc agcatccgga cgccacgctg
    11641 ccgcactatg acgctggcgg taagctgatg ctgcccacca cccgcgatat gcatattcat
    11701 ctcgacaaaa ccttctacgg cgggccgtgg cgctcgctca atcgtccggc aggcaccact
    11761 atccaggaca tgatcaaact cgagcagaaa atgctgccgg aactgcaacc gtacacgcag
    11821 gaacgggcgg aaaaactgat cgatttattg cagtcgaaag gcaccactat tgcccgcagc
    11881 cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg
    11941 gcgcgacgtc agacgggctt cgagtgtgag atcgtcgcct tcccgcagca cggtttgctg
    12001 ctgtcgaaat cggaatcctt aatgcgcgaa gcgatgcagg cgggggcgca ttacgtcggc
    12061 gggctggacc cgaccagtgt tgatggcgcg atggaaaaat ccctcgacac catgttccag
    12121 attgcgctgg actatgacaa aggcgtcgat attcacctgc acgaaaccac gccggcaggc
    12181 gtggcagcca tcaattatat ggttgaaaca gtggagaaaa cgccacagct gaagggcaag
    12241 ctgaccatca gccacgcctt tgcgctggca acgctcaacg aacaacaggt agatgaactg
    12301 gcgcaccgga tggcggcgca gcgaatttcg atcgcttcga cggtgccgat tggcacgctg
    12361 catatgccgc tcaaacagct gcacgacaaa ggcgtaaaag tgatgaccgg caccgacagc
    12421 gttatcgacc actggtcgcc ttacggcctg ggcgacatgc tggaaaaagc caatctgtac
    12481 gcgcagctct atattcgtcc taacgaacag aacctctccc gctctctgtt tttagccact
    12541 ggcgatgtat tgccgctcaa cgaaaaaggc gagcgcgtgt ggcccaaagc gcaggatgat
    12601 gccagttttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg tatttcgccg
    12661 agaacggcga cgttccataa agggcaactg gtgtggggga gtgtggcagg ttgatacgaa
    12721 cttgatatgt ttgcaacggt gaacagtaag agatttaagc cccagggagt ggggcggcct
    12781 gagtatgagt gtgtcggcag aaatggacat tatctttgaa gaatatgccc ttattgacgt
    12841 tgtgtagata actatttgtc tacgtgaaaa tatcatcagt tttgccgatt tttgtctttt
    12901 acggatagca aatatcacac ttataggcgc tcgccttatg gggaacgttg ctgctctgtt
    12961 tcattcaggc tcgcggcatg ttagggctgg atcgggtggt ttaaatattg gctatattca
    13021 atggacgcgt tttgccgcga tgacatatca ggcatcgtca aatacacata gctaatcagg
    13081 agtaaacaca atgaagatca aagctgttgg tgcatattcc gctaaacaac cgcttgaacc
    13141 gatggatatc acccggcgtg aaccgggacc gcatgatgtc aaaatcgaaa tcgcttactg
    13201 tggcgtctgc cattccgatc tccaccaggt ccgttcggag tgggcgggga cggtttaccc
    13261 ctgcgtgccg ggtcatgaaa ttgtggggcg cgtggtagcc gttggtgatc aggtagaaaa
    13321 acatgcgccg ggcgatctgg tcggtgtcgg ctgcattgtc gacagttgta aacattgcga
    13381 agagtgtgaa gacgggctgg aaaactactg tgatcacatg accggaacct ataactcgcc
    13441 gacaccggat gaaccgggcc atactctggg aggctactca caacagatcg tcgttcatga
    13501 gcgatatgtt ctgcgtattc gtcacccgca agagcagctg gcggcggtgg ctcctttatt
    13561 gtgtgcagga atcaccacgt actcgccgct acgccactgg caggccgggc cgggtaaaaa
    13621 agtgggcgtg gtcggcatcg gcggtctggg acatatgggg attaaactgg cccacgcgat
    13681 gggggcgcat gtggtggcat ttaccacttc tgagtcaaaa cgcgaagcgg caaaagccct
    13741 gggggccgat gaagttgtta actcacgcaa tgccgatgag atggtggctc atgtgaagag
    13801 tttcgatttc attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt
    13861 gctgaagcgc gatggcacca taacgctggt tggtgcgcct gcgacaccgc ataaatcgcc
    13921 ggaagttttc aacctgatca tgaaacgccg cgcgatagct ggttctatga ttggcggcat
    13981 tccagaaact caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga
    14041 gatgattcgg gccgatcaaa ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa
    14101 atatcgtttt gttatcgata atcgcacact aacagactga aaaaaattaa taaatatcct
    14161 gtggtttaac atattaattt cgctctcctt aactttttag ttaaggagag cgaaataata
    14221 tcaaagtagt ggtaaaccta tagcgtaaat tcagattgtt aaattaactc tactacttta
    14281 cacgttagtt atgagcgatt tctgatagtg tctggtttaa tcagagcttt attatctgcg
    14341 acgtttattt ttatttaaga gagccacctg caagttgtta ttccaccaga gagtgaatcg
    14401 caaagcaggt catcatttca tctgtatatc tgtatgccaa atggcattcc ccctcctttt
    14461 tcgccgatat tttgttgtga tcgacgaagt ttcgcttaaa aaagcaccag cagtggtttc
    14521 gcagctatat ggtgtataaa aaatgttctc atacagatgt tttgtgaatg tgtgggttga
    14581 cattcatatg aaaaaaatca taattccatc atgtttgtgt gggggctttt ctgtatctta
    14641 cgcatcgcac tcaagcctga cagaaaatat gctgtaaggc tcatatcaaa aaccgccatt
    14701 agctcatcag gaagagcaga cgacaaccag gattgttgta tggtacgggg ttcgaggcct
    14761 cgatggcggt ccaacttttc aaaactcttc tgaatcagat ccccttatta tcatccctgc
    14821 aaagtggaac attgccattg tttggctatt caggacgggt cagaccaatg aaaaagccca
    14881 cacaggggag agtaggctga aatgggaagc taaagactca agtaaactta tcggaaataa
    14941 ggaccacgca ttacgggggc tatcatcgcc gatggttggg attcggcaaa tcaggttgat
    15001 tacgtagaga gtaaattatc tgctcaccgc tgcgtcaccc cttcgtaaat caggcgtagc
    15061 gcgaatacac caataattgc accaataacc cgactggcaa cgcgttgcat acgcccataa
    15121 gcacgacgca cagcgggcaa agaaaacgcc tggcttagaa agactcgcca gataattgat
    15181 gtgagcacaa tccccgccca ggccattaat cgtgcccatg ttggtgtttc ggcatttaat
    15241 gttactgaga aaatactgat aaaaaataaa acggtttgcg ggttagagag atcggtaatt
    15301 aatccacggc ggaaaaagat ataccagggg gcactaatcg gttgttgtag tgtgctcatt
    15361 tgcggtgttg actggcggcg catgctgcac cacgcaaacc ataagagata agcgccgccg
    15421 acgattctga taagcgaaaa aatctcctca cactgcgtaa ttagcgttgc aagaccaaac
    15481 aaacccaacc cggaataaaa tgcatcgccc agcgccacgc ccagcccggt caacaccccc
    15541 gcgcgtcgac cggaagccag gctggtttgt actaccacaa agagattggc tcccggatta
    15601 aaaaaagtaa tcacgaacaa tcctacggtc aggtaaacgg cgtgcaaagg atccatagtg
    15661 atttcatcca taaataagtg aagtaacttc atcatatttc taccaaaaat aatcgggtgc
    15721 gagagagatc acaaagtgtc ttatttccgg ttactggcgt ttatgccctg actgaactaa
    15781 ttattaatca acccaataat gtgggtgggt gatagtgtga taacaactct ggagccgtaa
    15841 tatgaaaata atctctaaaa tgttagtcgg tgcgttagcg tttgccgtta ccaatgtcta
    15901 tgccgcagaa ttaatgacca aagcggaatt tgaaaaggtt gcatcgcaat atgaaaaaat
    15961 aggtgatatt tcaaccagta atgaaatgtc gaccgcagat gcgaaagaag atttgatcaa
    16021 aaaagccgat gaaaaagggg ctgatgtgtt ggtactgacc tctggtcaaa ctgacaataa
    16081 gatccacggc acggcaaata tttataagaa gaagtaattc tgaatcctat gtaaacatcc
    16141 ccgatgcgtt agtttatcgg tgatcatcat ctatcgaaat ttatgccgga taaagcgttc
    16201 gcgctgcatt cggcagttca gcttttcagc cgccgccaga acgtcgtccg gctgatacct
    16261 aaataattcg ccgctgctgt tttatcgcca ttaaatttct ccagtgcctg ttgtgctgtc
    16321 agcaagcctg gaatgggagt cttcgccgac tcgcgcgcca gttccggcag tagcagctgc
    16381 aaaaattgcg gcgttaaatc cggcgtcgat tccacactta aaaacagcgc cagtcgttcc
    16441 atcatattgc gcagttcacg aatattgccc ggccagtcgt agtgcaataa tagaatctga
    16501 caggtctcta atccctgacg taatgcggca gaaaaaggga cagagagtgc cgccagagac
    16561 attttcaaaa agctttccgc cagcggcaga atatccgcca cccgctcgcg tagcggcggc
    16621 agttgtagac gcaaaatact cagccgataa aacaggtcac ggcgaaactg cccttgctgc
    16681 atatcttctt ccagattgca gtgagtggcg ctaatgaccc gcacatccac cggaacaggc
    16741 tgatgcccgc cgacgcgggt gacctctttt tcttccagca cccgcagcag ccgagtctgc
    16801 aacggcagcg gcatttcgcc tatctcgtca agaaacagcg taccaccgtg ggcgatttca
    16861 aacagcccgg cgcgtcctcc gcgtcgcgag ccggtaaacg ccccttcctc atagccaaac
    16921 agctccgctt ccagcagcga ttcggcaatc gccccgcagt taacggcaac aaacggatgt
    16981 gactttttgc cctgtcgcgc atcgtggcgg gaaaaatatt cccgatgaat cgcctgggcc
    17041 gccagctctt tgcccgtccc cgtttccccc tcaatcaaca ccgccgcact ggagcgggca
    17101 tacagcaaaa tagtctgccg cacctgttcc atctgtggtg attgaccgag catatcgccc
    17161 agcacgtaac gagtacgcag ggcgttgcgg gtggcatcgt gagtgttatg gcgtaacgac
    17221 atgcgcgtca tatccagcgc atcgctgaat gcctggcgca cggtggcggc ggaatagata
    17281 aaaattccgg tcattccggc ttcttctgcc agatcggtaa tcagccctgc gccgaccacc
    17341 gcttcggtgc cgttggcttt caactcattg atttgcccgc gggcgtcttc ttcggtgatg
    17401 tagctacgtt ggtcgaggcg caaattaaag gtcttttgaa acgccaccag cgctggaatg
    17461 gtttcctgat aggtgacaac accgatagaa gaggtgagtt ttccggcttt tgccagtgcc
    17521 tgtaacacat cgtagccgct cggtttaatc aaaataactg gcactgacag gcgactttta
    17581 agatatgcgc cgttagagcc agcagcgatg atggcgtcgc accgttcgtt ggccagtttc
    17641 ttgcggatgt aggtcaccgc tttttcaaag ccaagctgga taggggtgat attcgccagg
    17701 tgatcaaact cgaggctgat atcgcgaaac agctcgaaca ggcgcgttac agataccgtc
    17761 cagataaccg gtttgtcgtc attcagccgt ggtggatgtg ccatagcgca ccgcaaagtt
    17821 aagaaaccga atattgggtt tagtcttgtt tcataattgt tgcaatgaaa cgcggtgaaa
    17881 cattgcctga aacgttaact gaaacgcata tttgcggatt acttcatgaa tttatcttta
    17941 actcattgaa attaaatgtt taattttatt aaggcaattg tggcacaccc attgctttgt
    18001 ctttatcaac gcaaataaca agttgataac aaaggatggg ctatgtctct acactctcca
    18061 ggtaaagcgt ttcgcgctgc actgactaaa gaaaatccat tgcagattgt tggcactatc
    18121 aacgctaacc atgcgctgct ggcgcagcgt gccggatatc aggcgattta tctctccggc
    18181 ggtggcgtgg cggcagggtc gctggggctg cccgatctcg gtatttctac tcttgatgat
    18241 gtgctgactg acattcgccg tatcaccgac gtttgttcgc taccgctgct ggtggatgcg
    18301 gatatcggtt ttggttcttc tgcttttaac gtggcgcgca ccgtgaaatc gatgattaaa
    18361 gccggtgcgg caggattgca tattgaagat caggttggtg cgaaacgctg cggtcatcgc
    18421 ccgaacaaag cgatcgtctc gaaagaggaa atggtggatc ggatccgtgc ggcggtggat
    18481 gcgaaaaccg atcctgattt tgtgatcatg gcgcgcaccg atgcgctggc ggtagagggg
    18541 ctggatgcgg cgatcgagcg tgcgcaggcc tatgttgaag cgggtgccga aatgctgttc
    18601 ccggaggcga ttaccgaact cgccatgtat cgccagtttg ccgatgcggt gcaggtaccg
    18661 atcctcgcca acattaccga atttggcgca acaccgctgt ttaccaccga cgaattacgc
    18721 agcgcccatg tcgcaatggc gctctacccg ctttcagcgt ttcgcgccat gaaccgcgcc
    18781 gctgaacatg tctacaacat cctgcgtcag gaaggcacac agaaaagcgt catcgacacc
    18841 atgcagaccc gcaacgagct gtacgaaagc atcaactact accagtacga agagaagctc
    18901 gacgacctgt ttgcccgtgg tcaggtgaaa taaaaacgcc cgttggttgt attcgacaac
    18961 cgatgcctga tgcgccgctg acgcgactta tcaggcctac gaggtgaact gaactgtagg
    19021 tcggataaga cgcatagcgt cgcatccgac aacaatctcg accctacaaa tgataacaat
    19081 gacgaggaca atatgagcga cacaacgatc ctgcaaaaca gtacccatgt cattaaaccg
    19141 aaaaaatcgg tggcactttc cggcgttccg gcgggcaata cggcgctctg caccgtgggt
    19201 aaaagcggca acgacctgca ttaccgtggc tacgatattc ttgatctggc ggaacattgt
    19261 gaatttgaag aagtggcgca tctgctgatc cacggcaaac tgccaacccg tgacgaactc
    19321 gccgcctaca aaacgaaact gaaagccctg cgtggtttac cggctaacgt gcgtaccgtg
    19381 ctggaagcct taccggcggc gtcacacccg atggatgtta tgcgcaccgg cgtttccgcg
    19441 ctcggctgca cgctgccaga aaaagagggg cataccgtct ctggcgcacg ggatattgcc
    19501 gacaaactgc tggcgtcact tagctcgatt cttctctact ggtatcacta cagccacaac
    19561 ggcgaacgca ttcagccgga aaccgatgac gactctatcg gcggtcactt cctgcatctg
    19621 ctgcacggcg aaaagccgtc gcaaagctgg gaaaaggcga tgcatatttc gctggtgctg
    19681 tacgccgaac acgagtttaa cgcttccacc tttaccagcc gggtgattgc gggcactggc
    19741 tctgatatgt attccgccat tattggcgcg attggcgcac tgcgcgggcc gaaacacggc
    19801 ggagcgaatg aagtgtcgct ggagatccag caacgctacg aaacgccgga cgaagccgaa
    19861 gctgatatcc gtaagcgggt ggaaaacaaa gaagtggtca ttggttttgg gcatccggtt
    19921 tataccatcg ccgacccgcg ccaccaggtg atcaaacgcg tggcaaagca gctctcgcag
    19981 gaaggcggct cgctgaagat gtacaacatc gccgatcgcc tggaaacagt gatgtgggag
    20041 agcaaaaaga tgttccccaa tctcgactgg ttctccgctg tttcctacaa catgatgggt
    20101 gttcccaccg agatattcac accactgttt gttatcgccc gcgtcactgg ctgggcggcg
    20161 cacattatcg aacaacgtca ggacaacaaa attatccgtc cttccgccaa ttatgttgga
    20221 ccggaagacc gccagtttgt cgcgctggat aagcgccagt aaacctctac gaataacaat
    20281 aaggaaacgt acccaatgtc agctcaaatc aacaacattc gcccggaatt tgatcgtgaa
    20341 atcgttgata tcgtcgatta cgtgatgaac tacgaaatca gctccagagt agcctacgac
    20401 accgcacatt actgcctgct cgacacgctc ggctgtggtc tggaagctct cgaatatccg
    20461 gcctgtaaaa aactgctggg gccaattgtc cccggcaccg tcgtacccaa tggcgtgcgc
    20521 gtccccggaa ctcagtttca gctcgacccc gtccaggcgg catttaacat tggcgcgatg
    20581 atccgttggc tcgatttcaa cgatacctgg ctggcggcgg agtgggggca tccttccgac
    20641 aacctcggcg gcattctggc aacggcggac tggctttcgc gcaacgcggt cgccagcggc
    20701 aaagcgccgt tgaccatgaa acaggtgctg accgcaatga tcaaagccca tgaaattcag
    20761 ggctgcatcg cgctggaaaa ctcctttaac cgcgtcggcc tcgaccacgt tctgttagtg
    20821 aaagtggctt ccaccgccgt ggtcgccgaa atgctcggcc tgacccgcga ggaaattctc
    20881 aacgccgttt cgctggcatg ggtagacgga cagtcgctgc gcacctatcg ccatgcgccg
    20941 aacaccggta cgcgtaaatc ctgggcggcg ggcgatgcca cttcccgcgc ggtacgtctg
    21001 gcactgatgg cgaaaaccgg cgaaatgggt tatccgtcag ccctgactgc gccggtgtgg
    21061 ggcttctacg acgtctcctt taaaggcgaa tcgttccgct tccagcgccc gtatggttct
    21121 tacgtgatgg aaaatgtgct gttcaaaatc tccttcccgg cggagttcca ctcccagacg
    21181 gcagttgaag cagcgatgac gctctatgaa cagatgcagg cagcaggcaa aacggcggcg
    21241 gatatcgaaa aagtgaccat tcgcacccac gaagcctgta ttcgcatcat cgacaaaaaa
    21301 gggccgctca ataacccggc tgaccgcgac cactgcattc agtacatggt ggcgatcccg
    21361 ctgctgttcg ggcgcttaac ggcggcagat tacgaggaca acgttgcgca agataaacgc
    21421 atcgacgctc tgcgcgagaa gatcaattgc tttgaagatc cggtgtttac cgctgactac
    21481 cacgacccgg aaaaacgcgc catcgccaat gccataaccc ttgagttcac cgacggcacg
    21541 cgatttgaag aagtggtggt ggagtaccca attggtcatg ctcgccgccg tcaggatggc
    21601 attccgaagc tggtcgataa attcaaaatc aatctcgcgc gccagttccc gactcgccag
    21661 cagcagcgca ttctggaggt ttctctcgac agaactcgcc tggaacagat gccggtcaat
    21721 gagtatctcg acctgtacgt catttaagta aacggcggta aggcgtaagt tcaacaggag
    21781 agcattatgt cttttagcga attttatcag cgttcgatta acgaaccgga gcagttctgg
    21841 gccgagcagg cccggcgtat tgactggcag acgcccttta cgcaaacgct cgaccacagc
    21901 aacccgccgt ttgcccgttg gttttgtgaa ggccgaacca acttgtgtca caacgctatc
    21961 gaccgctggc tggagaaaca gccagaggcg ctggcattga ttgccgtctc ttcggaaaca
    22021 gaggaagagc gtacctttac cttccgccaa ctgcatgacg aagtgaatgc ggtggcgtca
    22081 atgctgcgct cgctgggcgt gcagcgtggc gatcgggtgc tggtgtatat gccgatgatt
    22141 gccgaagcgc atattaccct gctggcctgc gcgcgcattg gtgctattca ctcggtggtg
    22201 tttgggggtt ttgcctcgca cagcgtggcg gcgcgaattg atgacgctaa accggtgctg
    22261 attgtctcgg ctgatgccgg ggcgcgcggc ggtaaaatca ttccgtataa aaaattgctc
    22321 gacgatgcga taagtcaggc acagcatcag ccgcgtcacg ttttactggt ggatcgcggg
    22381 ctggcgaaaa tggcgcgcgt tagcgggcgg gatgtcgatt tcgcgtcgtt gcgccatcag
    22441 cacatcggcg cgcgggtgcc ggtggcatgg ctggaatcca acgaaacctc ctgcattctc
    22501 tacacctccg gcacgaccgg caaacctaaa ggtgtgcagc gtgatgtcgg cggatatgcg
    22561 gtggcgctgg cgacctcgat ggacaccatt tttggcggca aagcgggcgg cgtgttcttt
    22621 tgtgcttcgg atatcggctg ggtggtaggg cattcgtata tcgtttacgc gccgctgctg
    22681 gcggggatgg cgactatcgt ttacgaagga ttgccgacct ggccggactg cggcgtgtgg
    22741 tggaaaattg tcgagaaata tcaggttagc cgcatgttct cagcgccgac cgccattcgc
    22801 gtgctgaaaa aattccctac cgctgaaatt cgcaaacacg atctttcgtc gctggaagtg
    22861 ctctatctgg ctggagaacc gctggacgag ccgaccgcca gttgggtgag caatacgctg
    22921 gatgtgccgg tcatcgacaa ctactggcag accgaatccg gctggccgat tatggcgatt
    22981 gctcgcggtc tggatgacag accgacgcgt ctgggaagcc ccggcgtgcc gatgtatggc
    23041 tataacgtgc agttgctcaa tgaagtcacc ggcgaaccgt gtggcgtcaa tgagaaaggg
    23101 atgctggtag tggaggggcc attgccgcca ggctgtattc agaccatctg gggcgacgac
    23161 gaccgctttg tgaagacgta ctggtcgctg ttttcccgtc cggtgtacgc cacttttgac
    23221 tggggcatcc gcgatgctga cggttatcac tttattctcg ggcgcactga cgatgtgatt
    23281 aacgttgccg gacatcggct gggtacgcgt gagattgaag agagtatctc cagtcatccg
    23341 ggcgttgccg aagtggcggt ggttggggtg aaagatgcgc tgaaagggca ggtggcggtg
    23401 gcgtttgtca ttccgaaaga gagcgacagt ctggaagacc gtgaggtggc gcactcgcaa
    23461 gagaaggcga ttatggcgct ggtggacagc cagattggca actttggccg cccggcgcac
    23521 gtctggtttg tctcgcaatt gccaaaaacg cgatccggaa aaatgctgcg ccgcacgatc
    23581 caggcgattt gcgaaggacg cgatcctggg gatctgacga ccattgatga tccggcgtcg
    23641 ttggatcaga tccgccaggc gatggaagag taggtcggat aaggcgctcg cgccgcatcc
    23701 gacaccgtgc gcagatgcct gatgcgacgc tgacgcgtct tatcatgcct acaggcctga
    23761 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc atgcgcagat gcctgatgcg
    23821 acgctgacgc gtcttatcat gcctacccac tgtttttaca cagataagtt tcccccgcct
    23881 ttttgtactc attcatataa gaaatatatc cccatacgaa aacgattgct ttttatcttc
    23941 agatgaatag aatgcggcgg attttttggg tttcaaacag caaaaagggg gaatttcgtg
    24001 tcgcaagata acaactttag ccaggggcca gtcccgcagt cggcgcggaa aggggtattg
    24061 gcattgacgt tcgtcatgct gggattaacc ttcttttccg ccagtatgtg gaccggcggc
    24121 actctcggaa ccggtcttag ctatcatgat ttcttcctcg cagttctcat cggtaatctt
    24181 ctcctcggta tttacacttc atttcttggt tacattggcg caaaaaccgg cctgaccacc
    24241 catcttcttg ctcgcttctc gtttggtgtt aaaggctcat ggttgccttc actgctgctg
    24301 ggcggcaccc aggttggctg gtttggcgtt ggcgtggcga tgtttgctat tccggtgggc
    24361 aaggcaaccg ggctggatat taatttgctg attgccgtct ccggtttact gatgaccgtc
    24421 accgtcttct ttggcatttc ggcgctgaca gttctttcgg tgattgcggt tccggctatc
    24481 gcctgtcttg gcggttattc cgtgtggctg gccgttaacg gcatgggcgg cctggacgca
    24541 ttaaaagcgg tcgttcccgc acaaccgtta gatttcaatg tcgcgctggc gctggttgtg
    24601 gggtcattta tcagtgcggg cacgctcacc gctgactttg tccggtttgg tcgtaatgcc
    24661 aaactggcgg tgctggtggc gatggtggcc tttttcctcg gcaactcgtt gatgtttatt
    24721 ttcggtgcag cgggcgcggc ggcactgggg atggcggata tctctgatgt gatgattgct
    24781 cagggcctgc tgctgcctgc gattgtggtg ctggggctga atatctggac caccaacgat
    24841 aacgcgctct atgcgtcggg tttaggtttc gccaacatta ccggtatgtc gagcaaaacc
    24901 ctttcggtga ttaacggtat tatcggtacg gtctgcgcat tatggctgta taacaatttt
    24961 gtcggctggt tgaccttcct ttcggcagct attcctccgg tgggcggcgt gatcatcgct
    25021 gattatctga tgaaccgtcg ccgctatgag cactttgcga ccacgcgtat gatgagtgtc
    25081 aattgggtgg cgattctggc tgtcgcgctg gggattgccg caggccactg gttaccggga
    25141 attgttccgg tcaacgcggt attaggtggc gcgctgagct atctgatcct taacccgatt
    25201 ttgaatcgta aaacgacagc agcaatgacg catgtggagg ctaacagtgt cgaataatgc
    25261 tttacaaaca attattaacg cccggttacc cggcaaagag gggctgtggc agattcattt
    25321 tcacgacgga aaaatcagcg ccattgatgc gcaatccggc gtgatgcccg taactgaaaa
    25381 cagcctggat gccgaacaag gtttagtttt accgccgttt gtggagccgc atattcacct
    25441 ggacaccacg caaaccgccg gacaaccgaa ctggaatcag tccggcacgc tgtttgaagg
    25501 cattgaacgc tgggccgagc gcaaagcgtt attaactcat gacgatgtga aacaacgcgc
    25561 atggcaaacg ctgaaatggc agattgccaa cggcattcag catgtgcgta cccatgtcga
    25621 tgtttcggac gcaacgctga ctgcgctgaa agcaatgctg gaagtgaaac aggaagttgc
    25681 gccgtggatt gatctacaaa tcgtcgcctt cccgcaggaa gggattttgt cgtatcccaa
    25741 tggtgaagcg ttgctggaag aggcgttacg cttaggggca gacgttgttg gggcgattcc
    25801 gcattttgaa tttacccgtg aatatggcgt ggagtcgctg cataaaacct tcgccctggc
    25861 gcaaaaatac gaccgtctca tcgatgttca ctgtgacgag attgatgatg agcaatcacg
    25921 ctttgtcgaa accgttgctg ccctggcgca ccgtgaaggc atgggcgcgc gagtcaccgc
    25981 cagccatact acggcaatgc actcctataa cggggcgtat acttcgcgcc tgtttcgttt
    26041 gctgaaaatg tccggtatta actttgtcgc caacccgttg gtcaatattc atctgcaagg
    26101 acgtttcgat acgtatccaa aacgtcgcgg catcacgcgc gttaaagaga tgctggagtc
    26161 cggcattaac gtctgctttg gtcacgatga tgtcttcgat ccgtggtatc cgctgggaac
    26221 ggcgaatatg ctgcaagtgc tgcatatggg gctgcatgtt tgccagctga tgggctatgg
    26281 gcagattaac gacggcctga atttaatcac ccaccacagt gccagaacgt tgaatttgca
    26341 ggattacggc attgccgccg gaaacagcgc caacctgatt atcctgccgg ctgaaaatgg
    26401 gtttgacgcg ctgcgccgtc aggttccggt gcgctattcg gtacgtggcg gcaaggtgat
    26461 tgccagcaca aaaccggcac aaaccaccgt atatctggag cagccagaag ccatcgatta
    26521 caaacggtaa tcggctgggt taccgcgagc gtagtttatg ccggatgcgg cgtgaacacc
    26581 ttatccggcc tacgtagatc actgaaattg taggcctgat aagcggagcg catcaggcaa
    26641 ttttgcttat ttaaactgaa gactcaactt taaaatcttt gaaataatat tgcttatccc
    26701 ggtcgtttat ttcgcgaata acccgacaag gaacgcctgc agccacgacg tttggtggaa
    26761 tgtcttttgt gacgacacta cccgcgccaa taacagaatt atccccgatg gtgatgcctg
    26821 gattaataac cacatgacat ccgatccaga cgttattgcc aatcgttatc ggaaaagagt
    26881 acatctctcc gttttttctc aattcatggt gtaccgggtg tcccgtaacg gaaagagtaa
    26941 cgttgggtgc aatcagtacg ttatcaccga tcgttaccgt gtagtcgtcg acaatggtta
    27001 aattgaaatt tgcataaaaa ttgcggccta tatggatgtt ggaaccgtaa gagaaataga
    27061 cgggggggtc tacccaggcg ttttccccta ccgtggcaaa catttcttta atcagacttt
    27121 cccttttttc aaattctgat ggatgcgagt gattaaactc atacattaac gtttttccac
    27181 gaagtctttt ttcaggtaag ccttcgcaca tatcggtaaa tagcttgcct gcttttattc
    27241 tttctgtcat cgacatgttc attgcgatca ttccgccctg atatgttggt cggataaggc
    27301 gctcgcgccg catccgacat tgattgctta agcgacttca ttcacctgac gacgcagtag
    27361 agaaagcggg ccggggccgc taagcgtgaa cacggaaatt aaggtgaagc ccagcgccac
    27421 cagacccagc accagataag cgccctggaa accgatgctt tcatacatat tgcccgccag
    27481 tacagacata aaaatcatcg ccagttgctt aaagaagcag aaacagacca gataaatcgt
    27541 cgctgaaaaa cgcacttcaa actggctggt aatatattta aagcagccca ccagcaggaa
    27601 cggtacttca aacatatgca gcgttttcag aataaccact tccagcgctg aggtggcgaa
    27661 cgatgagcca ataatacgta cagacataat agtgccagcc agcagcaggg catttttccc
    27721 accgatgcga ttaatgatca gtggcgcaaa gaacataatc gaggcattaa gtaattcgcc
    27781 cattgtcgtt acgtagccaa atacccgcgt accctgttca ccggtagcaa aaaacgaagt
    27841 aaagaaatta gcaaactgtt ggtcaaaaac atcgtaggtg caggaaacgc caataacata
    27901 cagtgacaaa aaccacagtt ttggctgtct gaacagttcc agcgccagct taaggctaaa
    27961 tgccgaatgg ttggcaccta ccgcattggc aaccgtggct gaagagggcg catccgtttt
    28021 ggcgaaaaag agtaaaacgg cgaggatgag tgcacagcca gaacccagcc agaaaacaaa
    28081 ctgattattg atggtgaaca tgatgccgac aatcgaggca cacagcgccc agccaacaca
    28141 gccaaacatc cgcgcgcgac caaattcgaa atgactgcga cggctgactt tctcgataaa
    28201 tgcctctact gctggcgcac cggcgttaaa acaaaagcca agataaatac caccaacaat
    28261 cgatcctact aaaatgttgt attgtaacag tggtccgaag ataaaaataa agaacggcgc
    28321 aaacatcact aacatgccgg taataatcca cagcaggtat ttgcgcagcc cgagtttgtc
    28381 agaaagcaga ccaaacagcg gttggaataa tagcgagaac agagaaatag cggcaaaaat
    28441 aatacccgta tcacttttgc tgatatggtt gatgtcatgt agccaaatcg ggaaaaacgg
    28501 gaagtaggct cccatgataa aaaagtaaaa gaaaaagaat aaaccgaaca tccaaaagtt
    28561 tgtgtttttt aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct
    28621 gcccggttat tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag
    28681 ctggaattcc gccgatactg acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa
    28741 accgtcgata ttcagccatg tgccttcttc cgcgtgcagc agatggcgat ggctggtttc
    28801 catcagttgt tgttggctgt agcggctgat gttgaactgg aagtcgccgc gccactggtg
    28861 tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta
    28921 cggggtatac atgtctgaca atggcagatc ccagcggtta aaacaggctg cagtaaggcg
    28981 gtcgggatag ttttcttgcg gccccaggcc gagccagttt acccgctctg cgacctgcgc
    29041 cagctggcag gtcaggccaa tccgcgccgg atgcggtgta tcgctcgcta cttcaacacc
    29101 tacactgacg accatctcgc cgtgtccatc aatccggtag gttttccggc tgataaataa
    29161 ggttttcccc tgatgctgcc acgcgtgggc ggtcgtaatc agcaccgcgt cggcaagtgt
    29221 atctgccgtg cactgcaaca acgccgcttc ggcctggtaa tggccagccg ccttccagcg
    29281 ttcgacccag gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag
    29341 cggcgcacgg gtgaactgat cgcgcagcgg ggtcagcagt tgtttttcat cgccaatcca
    29401 catctgtgaa agaaagcctg actggcggtt aaattgccaa cgcttattac ccagctcgat
    29461 gcaaaaatcc gtttcgctgg tggtcagttg agggatggcg tgggacgcgg aggggagtgt
    29521 cacgctgagg ttttccgcca gacgccattg ctgccaggcg ctgatgtgtc cggcttctga
    29581 ccatgcggtc gcgtttggtt gcactacgcg taccgttagc cagagttgtc cggcgctctc
    29641 cggctgcggc agttcaggca gttcaatcaa ctgcttacct tgcgggccaa catccagagg
    29701 cacttcaccg cttgccagcg gcttgccatc cagcgccacc atccagtgca ggaactcgtt
    29761 atcgctatga cggaacaggt attcgctggt cacttcgatg gttcgcccgg ataaacggaa
    29821 ctggaaatac tgctgttggt gttttgcttc cgtcagcgcc ggatgcggcg tgcggtcggc
    29881 aaagaccaga ccgttcatac agaactggcg atcgttcggc gtatcgccaa aatcaccgcc
    29941 gtaagccgac cacgggttgc cgttttcatc atatttaatc agcgactgat ccacccagtc
    30001 ccagacgaag ccgccctgta aacgggggta ctgacgaaac gcctgccagt atttagcgaa
    30061 gccgccaaga ctgttaccca tcgcgtgggc atattcgcaa aggatcagcg ggcgcatttc
    30121 tccaggcagc gaaagccatt ttttgatgga ccatttcggc accgccggga agggctggtc
    30181 ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtt gcggaggtgt cggctccgcc
    30241 gccttcatac tgtaccgggc gggaaggatc gacagatttg atccagcgat agagtgcgtc
    30301 gtgattagcg ccgtggcctg actcattccc cagcgaccag atgatcacac tcgggtgatt
    30361 acgatcgcgc tgcaccatcc gcgttacgcg ttcgctcatc gcgggtagcc agcgcggatc
    30421 atcggtcaga cgattcattg gcaccatgcc gtgggtttca atattggctt catccaccac
    30481 atacaggccg taacggtcgc acagcgtgta ccacagcgga tggttcggat aatgcgaaca
    30541 gcgcacggca ttaaagttgt tctgcttcat cagcaggata tcctgcacca tcgtctgctc
    30601 atccatgacc tgaccatgca gaggatgatg ctcgtgacgg ttaacgccgc gaatcagcaa
    30661 cggcttgccg ttcagcagca gcagaccatt ttcaatccgc acctcgcgga aaccgacatc
    30721 gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc agtttaacca ccgcacgata
    30781 gagattcggg atttcggcgc tccacagttc cggattttca acgttcaggc gtagcgtgac
    30841 gcgatcggca taaccgccac gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct
    30901 ggcgacctgc gtttcaccct gccacaaaga aaccgtcacc cgcagttcat cgcgcagctc
    30961 gccgtacatc tgaacttctg cctccagtac cgcgcggctg aaatcatcat taaagcgagt
    31021 ggtaacttgg aaatcgctga tttgcgtggt cggtttatgc agcaacgaga cgtcacggaa
    31081 aatgccgctc atccgccaca tatcctgatc ttccagataa ctgccgtcac tccagcgcag
    31141 caccatcacc gcgaggcggt tttctccggc gcgtaaaaat gcgctcaggt caaattcaga
    31201 cggcaaacgg ctgtcctggc cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc
    31261 cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac
    31321 attaaatgtg agcgagtaac aacctgtcgg attctccgcg ggaacaaacg gcggattgac
    31381 cgtaatggga taggttacgt tggtgtagat gggcgcgtcg taaccgtgca tctgccagtt
    31441 tgaggggacg acgacagtat cggcgtcagg aagatcgcac tccagccagc tttccggcac
    31501 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg
    31561 ggaagggcga tcggtgcggg cctcttcgct atttcgccag ctggcgaaag ggggatgtgc
    31621 cgcaaggcga ttaagttgtg taacgccagg gttttcccag tcacgacgtt gtaatacgac
    31681 ggccagagaa tccgtaatca tagtcatagc tgtatcctgt gtgaaattgt tatccgctca
    31741 caatttcaca caacatacga gccggacaca tagagtgtaa agactggggt gcctaatgag
    31801 tgagctgact cacattaatt gtgttgcgct cactgcccgc tttccagtcg ggaaacttgt
    31861 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    31921 ggaagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
    31981 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
    32041 tgtttgatgg tggttaacgg cgggatataa cacgagctgt cttcggtatc gtcgtatccc
    32101 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
    32161 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc atttagcatt
    32221 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc tgttatcggc
    32281 tgaatttgat tgcgtgtgag atatttatgc cagcccgcca gacgcagacg cgccgagaca
    32341 gaacttaatg ggcctgctaa cagcgcgatt tgctggtgac ccaatgcgac caggtgctcc
    32401 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg agtctggtca
    32461 gagacatcaa gaaataacgc cggaacatta gcgcaggcag cttccacagc aatggcatcc
    32521 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
    32581 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
    32641 cccagttgat cggcgcgaga ttttatcgcc gcaacaattt gcgacggcgc gtgcagggcc
    32701 agactggaag tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
    32761 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgct
    32821 gaaacgtggc aggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
    32881 tctgcgacat cgtatagcgt tactggtttc acattcacca ccctgaattg actctcttcc
    32941 gggcgctatc atgccatacc acgaaaggtt ttgcgccatt cgatggtgtc agcgtaaatg
    33001 cacgccgctt cgccttcccg ctaccagaat agcctgcgat tcaacccctt cttcgatctg
    33061 ttttgccacc cgttgcagcg ccggaagatg cttttccgct gcctgttcga tggtcattgc
    33121 gctcgccata tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac
    33181 ggcgatagag gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtacccctc
    33241 tttgcgcgcg cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc
    33301 atcaccgggg cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa
    33361 ggccagccag gtcaggcccg aggcggtttt cagaagcggt aaccgtcgcc cgaccattgc
    33421 ccggtgaaaa gataagcggc tgaaacgatg ggtggtttcg cgtaccacca ttgcatcaac
    33481 atccagcgtg gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagcgg
    33541 ggccgccagt gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt
    33601 gatggtcaga cgaaaactgt catcggaggt actgcgccgg acatatccct cttccagtaa
    33661 cgtctccagc agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc
    33721 gacgctggca ccgccatcaa gtttatttaa catatttaat aacattagac cgcgggttaa
    33781 gccgcgcacg gttttgtatt ccgtctgctc attgttctgc atattaattg acatttatat
    33841 agttaaaaca acgttgtgca cctggtgcac
//
LOCUS       DAJOTB010000035        33541 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJOTB010000035 DAJOTB010000000
VERSION     DAJOTB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.035"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            62..628
                     /locus_tag="OHN28_001701"
     CDS             62..628
                     /locus_tag="OHN28_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2297108.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RLRIALIFDVGVSTIYRKFPANKSNESP"
     gene            771..1943
                     /locus_tag="OHN28_001702"
     CDS             771..1943
                     /locus_tag="OHN28_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCQ2297109.1"
                     /translation="MAQEYNHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLGVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDDQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDTATDAGLLNQNDVTTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            1953..2468
                     /locus_tag="OHN28_001703"
     CDS             1953..2468
                     /locus_tag="OHN28_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCQ2297110.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            2523..2825
                     /locus_tag="OHN28_001704"
     CDS             2523..2825
                     /locus_tag="OHN28_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCQ2297111.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSIEVVTFLLKKSVLAGLPTA"
     gene            2840..2959
                     /locus_tag="OHN28_001705"
     CDS             2840..2959
                     /locus_tag="OHN28_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCQ2297112.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            2952..6029
                     /locus_tag="OHN28_001706"
     CDS             2952..6029
                     /locus_tag="OHN28_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2297113.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIRNSRDALK
                     QLNQAGNSLEKFRKLQVDNKKLGDRLNYARQKANLLSSELESMEQPSQRHLVALGRQT
                     LAVQRLEEQQKYLQKQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGTPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNI
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     CGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAALRGGLAAAGAVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNTDGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAMVSRAATVPPA
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6026..6511
                     /locus_tag="OHN28_001707"
     CDS             6026..6511
                     /locus_tag="OHN28_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297114.1"
                     /translation="MMMVLGLYVFMLRTAPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            6508..7608
                     /locus_tag="OHN28_001708"
     CDS             6508..7608
                     /locus_tag="OHN28_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCQ2297115.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            7699..7917
                     /locus_tag="OHN28_001709"
     CDS             7699..7917
                     /locus_tag="OHN28_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCQ2297116.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8154..9839)
                     /locus_tag="OHN28_001710"
     CDS             complement(8154..9839)
                     /locus_tag="OHN28_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCQ2297117.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10108..10485
                     /locus_tag="OHN28_001711"
     CDS             10108..10485
                     /locus_tag="OHN28_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCQ2297118.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(10515..10772)
                     /gene="grxA"
                     /locus_tag="OHN28_001712"
     CDS             complement(10515..10772)
                     /gene="grxA"
                     /locus_tag="OHN28_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCQ2297119.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10932..11219
                     /locus_tag="OHN28_001713"
     CDS             10932..11219
                     /locus_tag="OHN28_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCQ2297120.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            11203..11925
                     /gene="nfsA"
                     /locus_tag="OHN28_001714"
     CDS             11203..11925
                     /gene="nfsA"
                     /locus_tag="OHN28_001714"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCQ2297121.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11986..12888
                     /gene="rimK"
                     /locus_tag="OHN28_001715"
     CDS             11986..12888
                     /gene="rimK"
                     /locus_tag="OHN28_001715"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCQ2297122.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12976..13452
                     /locus_tag="OHN28_001716"
     CDS             12976..13452
                     /locus_tag="OHN28_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCQ2297123.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            13802..14914
                     /gene="potF"
                     /locus_tag="OHN28_001717"
     CDS             13802..14914
                     /gene="potF"
                     /locus_tag="OHN28_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCQ2297124.1"
                     /translation="MIALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15009..16142
                     /gene="potG"
                     /locus_tag="OHN28_001718"
     CDS             15009..16142
                     /gene="potG"
                     /locus_tag="OHN28_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCQ2297125.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16152..17096
                     /gene="potH"
                     /locus_tag="OHN28_001719"
     CDS             16152..17096
                     /gene="potH"
                     /locus_tag="OHN28_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCQ2297126.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            17093..17938
                     /gene="potI"
                     /locus_tag="OHN28_001720"
     CDS             17093..17938
                     /gene="potI"
                     /locus_tag="OHN28_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCQ2297127.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            17998..18486
                     /locus_tag="OHN28_001721"
     CDS             17998..18486
                     /locus_tag="OHN28_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCQ2297128.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            18527..19654
                     /gene="rlmC"
                     /locus_tag="OHN28_001722"
     CDS             18527..19654
                     /gene="rlmC"
                     /locus_tag="OHN28_001722"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCQ2297129.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(19683..20414)
                     /gene="artJ"
                     /locus_tag="OHN28_001723"
     CDS             complement(19683..20414)
                     /gene="artJ"
                     /locus_tag="OHN28_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCQ2297130.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20640..21308)
                     /gene="artM"
                     /locus_tag="OHN28_001724"
     CDS             complement(20640..21308)
                     /gene="artM"
                     /locus_tag="OHN28_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCQ2297131.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(21308..22024)
                     /gene="artQ"
                     /locus_tag="OHN28_001725"
     CDS             complement(21308..22024)
                     /gene="artQ"
                     /locus_tag="OHN28_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCQ2297132.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(22031..22762)
                     /gene="artJ"
                     /locus_tag="OHN28_001726"
     CDS             complement(22031..22762)
                     /gene="artJ"
                     /locus_tag="OHN28_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2297133.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22780..23508)
                     /gene="artP"
                     /locus_tag="OHN28_001727"
     CDS             complement(22780..23508)
                     /gene="artP"
                     /locus_tag="OHN28_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCQ2297134.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23726..24241)
                     /locus_tag="OHN28_001728"
     CDS             complement(23726..24241)
                     /locus_tag="OHN28_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2297135.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24367..24690
                     /locus_tag="OHN28_001729"
     CDS             24367..24690
                     /locus_tag="OHN28_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCQ2297136.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            24687..25517
                     /gene="amiD"
                     /locus_tag="OHN28_001730"
     CDS             24687..25517
                     /gene="amiD"
                     /locus_tag="OHN28_001730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCQ2297137.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(25514..26527)
                     /locus_tag="OHN28_001731"
     CDS             complement(25514..26527)
                     /locus_tag="OHN28_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2297138.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(26626..28056)
                     /locus_tag="OHN28_001732"
     CDS             complement(26626..28056)
                     /locus_tag="OHN28_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCQ2297139.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(28067..29068)
                     /gene="ltaE"
                     /locus_tag="OHN28_001733"
     CDS             complement(28067..29068)
                     /gene="ltaE"
                     /locus_tag="OHN28_001733"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCQ2297140.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNMLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29105..30823)
                     /gene="poxB"
                     /locus_tag="OHN28_001734"
     CDS             complement(29105..30823)
                     /gene="poxB"
                     /locus_tag="OHN28_001734"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCQ2297141.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(30956..31924)
                     /gene="hcr"
                     /locus_tag="OHN28_001735"
     CDS             complement(30956..31924)
                     /gene="hcr"
                     /locus_tag="OHN28_001735"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCQ2297142.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            complement(31936..>33541)
                     /gene="hcp"
                     /locus_tag="OHN28_001736"
                     /gene_synonym="priS"
     CDS             complement(31936..>33541)
                     /gene="hcp"
                     /locus_tag="OHN28_001736"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCQ2297143.1"
                     /translation="GCSYAQGMCGKTAETSDLQDLLIATLQGLSAWAVKAREYGIINH
                     DVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQCLAVDANARVDNPMADL
                     QLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGAAAYMEHAHVLGQYD
                     NDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDAGETGKYGHPTPTQV
                     NVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAHGYPELRKFKHLVGN
                     YGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIVGWPGVRHLDGEDFS
                     AVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSREKLRHIFLLGGCDGA
                     RGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRLVDAGQCNDAYSAII
                     LAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNIVTGPTAPGFLTPDL
                     LAVLNEKFGLRSITTVEEDMKQLLSA"
BASE COUNT         7966 a         8505 c         9163 g         7907 t
ORIGIN      
        1 agaaaatgca gagattacgg ctaactggca tcatctccgg tttttattca gggggatgat
       61 catgcttatt ggctatgtac gcgtgtcaac aaatgaccag aacaccgatt tgcaacgtaa
      121 tgcactgaac tgcgcgggat gtgagcggat ttttgaggat aaaatcagtg gcactaagtc
      181 cgacagaccg gggctgaaaa aactgctcag gacactatcg gcaggagaca cgctggttgt
      241 ctggaagctg gacaggttgg ggcgcagtat gcggcatctt gttacgctga tagaagagtt
      301 gcgccagcgt ggtgtgaatt tcagaagcct gactgacagt attgatacca gtaccccaat
      361 gggccgtttc ttttttcatg tcatgggtgc cctggctgaa atggaacgcg aactgatagt
      421 tgaacgtacc agggcagggc tggctgcagc tcgtgctaaa ggcagagtag gtggacgccg
      481 tcctaagttg accaccgaac agtgggcaca gattggacgt ttactcgagg ccggagaatc
      541 aagactgcgt attgcactga tttttgatgt aggcgtttct accatttata gaaaatttcc
      601 ggcaaataag agcaatgaat ctccctgaat cagcattatt ttgattatcc ctgcaagcag
      661 acaaataccg tcattttgtg tgaataacgg tacaactgcg cttagctgtt tgtcaggcac
      721 aatcacttca acatagggcg aagcctaatc caatcaggag gttcgccact atggctcagg
      781 aatacaacca cggggtgcgc gttgttgaag tcaacgaagg cacccgatcc attaccacgg
      841 tgagcaccgc catcgtgggt atggtctgca cgggcgatga tgccgatgca aaaatgtttc
      901 ctcttaataa acccgtgctg atcactgatg tgctgactgc cagcggtaaa gcgggtgagt
      961 ccggcacgct ggcccgttcg ctggatgcca tcgctgacca ggcaaaaccc gtgaccgttg
     1021 ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac gaccaatatc atcggcgcag
     1081 tgactgctga aggtaaaaaa acaggtatga aagccctgtt atctgcccag tcacagctcg
     1141 gcgttaaacc gcgcattctc ggcgtgccag gccacgacac caaggcggta gctactgagt
     1201 tgctgggcgt ggcgcaaagc ctgcgtgggt ttgcttacct gtcagcgtat ggctgcaaga
     1261 ctgtgcagga ggcgatcact taccgcgaaa acttcagcca gcgcgaaggg atgctgatct
     1321 ggcctgactt tactggctgg gacacggtgc tgaatgccga agcaacggct tatgccaccg
     1381 cccgtgcgct tggtctgcgt gccaaaattg atgatcagac cggatggcac aaaagcctgt
     1441 ccaatgtggg cgttaacggt gtcactggaa tttctgctga tgtgttctgg gatctgcagg
     1501 acacggcaac cgatgctgga ctgctgaacc agaacgacgt caccaccctt atccgcaaag
     1561 acggtttccg cttctggggt tcccgctgcc tgagtgatga cccgctcttt gccttcgaaa
     1621 actacacccg cacggcgcag gtgctgatgg acacgatggc agaagcacac atgtgggcag
     1681 tggacaaacc gctgaacccg tcgctggcgc gcgacattat cgagggcatc cgcgccaaaa
     1741 tgcgcagcct ggtcagtcag gggtatctca ttggtggtga ttgctggctg gacgagtcgg
     1801 tgaacgacaa agacactctg aaagccggaa aactcaccat cgactacgac tacacgccag
     1861 tgccgccact tgaaaacctg atgttgcgtc agcgcatcac cgatcagtac ctggtgaatt
     1921 tcgccagcca ggtcagcgcg taaggggaca acatggcttt accacgcaaa ttaaaacacc
     1981 tgaacctgtt taacgacggg aacaactggc aggggatcgt tgagtcgctg acgctgccga
     2041 aattcacccg caaatatgag aagtatcgcg gcggcggaat gccgggggca gtggatgtgg
     2101 atctggggct tgatgacagt gcgctggaca cagaattttc cattggtggt actgaactgc
     2161 tgctgtttaa gcagatgggc aaagccacgg tggatggcat ccagctgcgc tttaccggct
     2221 ctatccagcg tgacgatacc ggggaagtgc aggccgtgga gcttgtggtg cgtggacgtc
     2281 acaaagaagt ggattccggt gagtggaaga cgggcgaaag caacaccacc aaagtgacca
     2341 gtaccaacag ctacgcgaag ctgaccatca atggtgaggt gctctatgaa gtggacctta
     2401 tcaacatggt ggaaattgtg gacggtgtgg acctgatgga agcgcaccgc aacgccctcg
     2461 gcctctgatg tatctgaacg gcgcgggata ccgcgccaga acccaattta caggacagca
     2521 aaatgagcga taagcagact gaaaagacca ttcaactgga tacccctatc aagcgcggta
     2581 aaacagaaat caccgaaatt gtgctgcgta aaccgcagtc cggtgcgctg cgcggtacac
     2641 gcctgcaggc cattatggat atggatgtga acgcgatgat gaccgtgatc ccccgcatct
     2701 ccagcccggc actgaccgca caggaaattg cagagatgga cccggcagat ctcactgcca
     2761 tgtcgattga ggttgtcact tttttgttga agaagtcggt gcttgccggt ttaccgacag
     2821 cctgacggtt gacgatctgg tggcagatat tgccaccatc tttcactggc cgccatccgt
     2881 tactgacgtt atgccgctga ccgaagtgct ggaatggcgg tataaagcga ttcagagaag
     2941 cggggccaac gatgagtgat aataacctgc ggctgcaggt cattcttaat gcggttgaca
     3001 aactcacccg cccattccgt gctgcacagg ccagttcgaa agagctggct ggcgcaatca
     3061 gaaactcccg tgacgcatta aagcaactca atcaggcggg taacagcctg gaaaaatttc
     3121 gcaagctgca ggtcgataac aagaagttag gcgacaggct gaactatgcc agacagaagg
     3181 ctaatttgct tagctctgag ctggagtcga tggaacaacc atcacaacgg caccttgtgg
     3241 ctttaggtcg gcaaacgctg gcagtccaac gcctggaaga acaacaaaaa tatttgcaga
     3301 agcaaacggc gcttgtgcgt gcagaactgt accgggcggg aatttctgcg aaagatgatg
     3361 cgggagcaac tgcccgttta gcccgtgaaa catcacgtta taaccaggaa ctgtcgaaac
     3421 aggaggcgcg gctgaagcga ctgggggaag ctcagcgcag gatgaatgca gcgcgtgcca
     3481 gttatgctcg ttctctggag gtgcgcgatc gcatcgcagg agccggagcc accaccacgg
     3541 ctgcagggct ggcaatgggg acgccagtga tggcggcagt aaaaagctat accagcatgg
     3601 aagatgccat gaaaggtgtg gcaaagcagg tcaatggtct gcgtgacgat aatggcaacc
     3661 gcactgcacg tttttatgaa atgcaggatg ccatcaaagc tgccagcgaa cagttgccga
     3721 tggaaaacgg tgcggtggac ttcgccgcac tggttgaagg tggtgcgcgc atgaacatcg
     3781 caaaccctga cgacagctgg gaagaccaga aacgtgatct gctggccttc gccagcacgg
     3841 cagcaaaggc ggcaacagcc tttgagttgc ctgcggatga actgtcagaa agtctgggga
     3901 aaatcgccca gctctacaaa atccctaccc gcaatattga acagctcggt gatgcgctga
     3961 actatctgga tgataacgcc atgtcgaaag gggcagacat cattgatgtc atgcaacgtc
     4021 tgggcggtgt ggctgatcgt ctggattatc gtaaagcggc ggcgctgggt tccaccttcc
     4081 ttacactggg cgctgcgcca gaggttgcag ccagtgcagc aaacgcgatg gtgcgtgaat
     4141 tgtccattgc caccatgcaa agcaagagtt tctttgaagg gatgaatctg ctgaaactca
     4201 atcctgaagt gattgaaaag cagatgacga aggatgcgat gggaaccatc cagcgcgtgc
     4261 tggagaaggt gaacgcactg ccgcaggata agcgtctgtc tgccatgacc atgttgtttg
     4321 gtaaagagtt tggcgatgac gcggcgaaac tggcaaacaa cctgccggaa ctgcagcgtc
     4381 agttaaaact gacagcgggc aatgatgcgc tcggctccat gcagaaagaa tccgacatca
     4441 acaaggattc actttctgcg cagtggttgc tggtcaaaac cggagcacag aacaccttca
     4501 gcagcctggg cgaaacgctg cgccagccgc tgatggatat tctgtacacg gtgaaaagcg
     4561 tcacaggggc gttgcgtcgc tgggtggaag ctaacccgga actgacaggc acactgatga
     4621 aagtagccgc tgtggtggct gccgttaccg tgggcctcgg caccttagcg gtggcgctgg
     4681 ctgcagtgct ggggccgctg gcagtgatcc gtctgggatt ctctgtgctg ggtatcaaaa
     4741 cgttaccttc cgttacggca gcagtaactc gaaccagcag cgcgttgtcc tggctggctg
     4801 gcgcaccact ggcactgctg cgatgcgggc ttgcttcatc gggcaacgcc gcaggtttac
     4861 ttactgcgcc gttgtcgtct ttgcgccgca cggcatcact gacgggaaat gtcctgaaaa
     4921 cggtagcagg tgcgccggtt gcacttttgc ggtctggatt atccggttta cgtgcggttg
     4981 ctgtgatgtt tatgaatcca ctggcagcac tgcgcggtgg actggctgcc gcaggcgcgg
     5041 tgctgcgtgt gctggcatcc ggtccgctgg cgatgctgcg cgttgccctg tatgccgtat
     5101 ctggtctgtt aggtgctctg ctcagtccga taggtcttgt ggttactgca ctggcgggcg
     5161 tggcgctggt tgtctggaaa tactggcaac ccatcaccgc atttctcggt ggcgtggtgg
     5221 aaggattcaa agcggcggca ggtcccatca gtgctgcatt cgaaccgctt aagcccgtgt
     5281 tccagtggat tggcgacaaa gtacaggcgt tgtggggctg gtttactgat ctgctgacgc
     5341 ccgttaagtc gacctctgcc gaactgcaga gcgcagcggc aatggggcga cgattcgggg
     5401 aggcgctggc ggaagggctg aatatggtca tgcatccgct ggactcgctg aaatccggcg
     5461 tttcctggtt gctggagaaa cttggcattg tcagtaaaga ggccgcaaag gcaaaactgc
     5521 cggaaagcgt gacgcgtcag caacctgcga cggtgaatac agacggtaaa gtgatgatgc
     5581 catcaggtgg ttttccatca tggggatatg gttttgcggg gatgtatgac agcggtgggt
     5641 atatcccgcg cgggcagttt ggcattgtcg gtgagaacgg gccggaaatt gttaacggcc
     5701 cggcaaatgt gaccagccgg agaaatacag ctgcactggc tgccgttgtt gccggaatga
     5761 tgggcgttgc tgccgcgcct gcagagcttc cgccgttaca tcctttggca cttcccgcga
     5821 aaggcggcga agcgatggtg agtcgtgcag ccactgtgcc gcccgctcaa cggattgagg
     5881 caccgacgca gatcatcatc cagacgcagc caggacaaag tgcgcaggat attgcgcggg
     5941 aggtggcccg acagcttgat gaacgtgaac gcaggctgaa ggcaaaagcc aggagtaact
     6001 acagcgatca ggggggatac gacgcatgat gatggtgctg ggattgtacg tgtttatgct
     6061 gcgcaccgcg ccgtatcagg aactgcagta tcaacgcagc tggcgacatg cggcaaacag
     6121 ccgggtaaac cgacgtccgt ccacgcagtt tctgggaccg gacaacgaca tgctgacgct
     6181 ttccggtgtt cttatgccgg agataacagg tggcaggctg tcgttgctgg cacttgagca
     6241 gatggcagaa caggggaaag catggcccct gattgaaggc agcggcacga tttacggcat
     6301 gtatgtgatt gagggactga atcagactaa aacggagttt ttccgcgacg gtatgccgcg
     6361 ccggattgag ttcactctgt cgctcaaacg ggtggatgaa tccctgtccg atatgttcgg
     6421 tgatctcagt gcgcagctga ataatctgca ggacacggca acgtctgcct taagcgatat
     6481 cagtaaaacg gtgggagggc tgctgtcgtg aatttcagct ctgaactgct taacaaaggc
     6541 aacaaaactc cggcattcag catcagtatt gaaggcaggg atatcaccac tgtgctggac
     6601 aaccgcctga tggggctgac gctgacggat aaccggggct ttgaagcaga ccagcttgat
     6661 ctggagctgg acgacgccga cggaaaaatc gtgctgccgc gccgtggtgc ggtcattacg
     6721 ctggcgctgg gctggaaggg gcagccgctt ttcccgaaag gagcattcac ggtggacgag
     6781 attgaacaca ctggcgcacc ggaccgcctg actatccggg cgcgaagtgc tgattttcgt
     6841 gaaaccctga atacccgccg tgaaaaatcg tggcacaaga ccaccgttgg ggaagtggtg
     6901 aaggaaatag ctgcgcggca caaactgaag atggcattgg gtgaagacct gtcggataaa
     6961 cccgtggagc atatagacca gaccaatgag agtgacggca gttttctgat gcggctggcg
     7021 cgccagtacg gtgctattgc gtcggtgaaa aatggcaatc tgttattcat ccggcaggga
     7081 cagggtaaaa gcgccagcgg taaaccactg ccggtgatca ctatcacacg gaaggacggc
     7141 gacagtcacc gctttaccct ggcagatcgc ggagcctaca cgggcgtaat tgccagctgg
     7201 ttgcataccc gcgaacccgc gaagaaagaa agcaccacgg tgaagcgtaa gcgcaggact
     7261 aagaagcaga agaaagagcc ggaagcgaag cagggcgatt acctggtggg gacggatgaa
     7321 aacgtgctgg tacttaatcg cacttatgcc aaccggagca acgccgaacg agcggcgaaa
     7381 atgcagtggg aacgcctgca acgcggtgtt gcgtcattct cgctacaact ggcggaaggt
     7441 cgggcagatc tctacacgga aatgcctgtg aaggtcagtg gctttaaaca gccgatagat
     7501 gatgcggaat ggaccattac caccctgaca catactgtca gcccggataa tggttttacg
     7561 accagtctgg agcttgaagt gaggattgat gatttcgaaa tggaatgatt cttcgcaatg
     7621 gagaactttt aagtttgcaa aatggaataa tgcggtatca ttattgtgaa tttagcaaaa
     7681 atggggagaa ctcgaaaaat gatgatttgc ccactgtgtg gaagtgccgc ccatactcgc
     7741 agcagttttc aggtatcttc attgaccaaa gagcgttaca accagtgcca gaacattaac
     7801 tgcagccata cttttgttac ccatgaaact tttgttcgtt cgattgcaac gccaaaagag
     7861 tcaaatccgg ttcagccgca tccaatgaaa tcaggacagg tggcgctctc tctttgacgc
     7921 tgccgccatt ttgtcgccat cgttaaaaaa cagtgtttct aacatcatga ttttaaacag
     7981 cttaaatttc aggcaacaaa aaacccatca accttgaacc gaaatggcgg ggttgatggg
     8041 ctccacaaaa tggggacatc aaagaaaagc agtggcacta attaagactg atgccctgcg
     8101 gaaaagttct gcggttgtgc aaaaaaaatt tcattttcag ggcaacttca gttttatcct
     8161 aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac gttggcgatt
     8221 gcataggtgc ccgcatagcc cagcgccggg atgttactgc gagctgtatc actgatgatc
     8281 tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc
     8341 attcgcaata cataagcacc gaacaagaaa cagataacca ctggcacaag actgacaatc
     8401 aatccggcaa tcaacatctg accgccaatc gcgcccaggc cgttattaat accgctaccg
     8461 gcgctcagac caacgcctgc cataaacacc attaagccga actctttcac catgcttaat
     8521 gcaccctgcg gaatgtaacc gaaggtcggg tggttagcgc gcataaatcc cagcataatt
     8581 ccggcgaata acaacccggc agcgtttccc atgccgaaac tgaatgtgct gaactggaag
     8641 gtgatcatcc cgatcatcag cccaataaca aagaaggcgc agaatgccag caagtcagtg
     8701 acctggctat gaatcgagat aaagccgatg cgatcggcga tggtttttac gcgacgggca
     8761 tcgccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc tatcggcatc
     8821 tcaatctggc tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt
     8881 gcgagacgtt tgccaacggc gttatggttt ttaacgacca cttcttcagt gacgatacgc
     8941 atgtcgagaa ggtcacgatc gaaaacttct ttgccgttac ggaagctggg atcgagtcgg
     9001 gcgtgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag caccgcatca
     9061 ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca gccagtttgt
     9121 cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag ctccgggccg
     9181 acgcggtagg cgcggatcac cggtaaataa accttacggt tggcatcagt gtccaggcca
     9241 cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag
     9301 taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc atacccgagg
     9361 ctcagattat ccagtgccag tgagagctgc ctgctttcca tgccggaatg acgcagtgta
     9421 tcgccagcac cgaccagaac cggtgtcgac gtcatagagc cagctaacat accggccgtc
     9481 aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag cgcactgcca
     9541 accatcacca gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag
     9601 ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag attaagcgcg
     9661 tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa aacgccaatg
     9721 gaattaccca gttggatcga accaagtcgt aatttcccga gacatagccc aagcgcgagg
     9781 accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt tatattcacg
     9841 gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tggtaattta cgataatgtt
     9901 tttaccagaa ttcagggcgc agattcattc agcgcaccta aacgatagta aagtaacaat
     9961 atattttact agtgtaatca cattaggtat caacggctat atgaattgcg ttggcctata
    10021 ttagcatgga atgcgaagcg gctttatctt actgaacgcc acactggcga aaaatgtgtt
    10081 cgatagacgc agtgtcagga ggaacgagtg aaacataaac aacgttgggc gggggcaatc
    10141 tgctgttttg tcctcttcat tgtggtgtgt ctttttctgg cgacgcacat gaaaggcgct
    10201 tttcgggctg ccgggcatcc tgaaatcggt ttgctatttt tcattcttcc tggtgcagtt
    10261 gccagcttct tttcacatcg tagagaagtc ctgaaacctc tgtttggcgc aatgctggcg
    10321 gcaccctgct cgatgctcat tatgcggctg tttttttcac cgacgcgctc attctggcaa
    10381 gagctggcat ggttattaag tgcggtgttc tggtgtgcgc tgggggcatt gtgtttctta
    10441 tttatcagta gtttgtttaa accacagcac agaaaaaatc aataaagccc tcaatgcgag
    10501 ggcttgtcag acgatcaggc gtccagattc tctttcaccc atgcagcaaa atcggtatag
    10561 ccgccgatat gttgctgatc gacaaaaatc tgcggcacgg tttctacggg tttacctgcc
    10621 ttttgttgta gatcttcttt agtgatccct tccgcacgaa tatctacata ctgatactgg
    10681 aaatcatcgc gttcattgct caatttctca gccagatctt ttgcacgcac acagtaaggg
    10741 caacccggac gaccaaaaat aacggtttgc attatttctc tcctcataga tttatgcctg
    10801 taatgatcac gctaaaatgt attcgctgaa agtaggttta acctgttgca ttaattgcta
    10861 aaagctataa ctgttaaaca caatacagtg aaaagtttta gactgaaggc tcactttgca
    10921 gagggaagcg tatgcgcgcg atcggtaaat tgcctaaaag cgtgttgata ctggaattta
    10981 tcggaatgat gttgctggcg gtggcgctgc tgtcggtaag cgactccctg tcgctacctg
    11041 agccattttc tcggccagaa gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc
    11101 ttcccgctgc ggtggtggtt attcttcagg tggcaaaacg tcttgcccca cagctgatga
    11161 accatccacc gcaatattca cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa
    11221 cttatttgtg gccatcgctc cattcgccat ttcactgatg aacccatttc cgaagcgcag
    11281 cgtgaggcga ttattaacag cgcccgtgcg acgtccagtt ccagtttttt gcagtgcagt
    11341 agcattatcc gcattaccga caaagcgtta cgtgaagaac tggtgacgct gaccggcggg
    11401 caaaaacacg tagcgcaagc ggcagagttt tgggtgttct gtgccgactt taaccgccat
    11461 ttacagatct gtccggatgc tcagctcggc ctggcggaac aactgttgct cggtgtcgtt
    11521 gatacggcaa tgatggcgca gaatgcatta accgcagcgg aatcgctggg attgggcggg
    11581 gtatatatcg gcggcctgcg caataatatt gaagcggtga cggaactgct gaaattaccg
    11641 cagcatgttc tgccgctgtt tgggctgtgc cttggctggc ctgcggataa tccggatctt
    11701 aagccgcgtt taccggcctc cattttggtg catgaaaaca gctatcaacc gctggataaa
    11761 gacgcactgg cgcagtatga cgagcaactg gcggaatatt acctcacccg tggcagcaat
    11821 aatcgccggg atacctggag cgatcatatc cgccgaacaa tcattaaaga aagccgccca
    11881 tttattctgg attatttgca caaacagggt tgggcgacgc gctaaaactg ccacgtcgat
    11941 gtatgatacg cgggcttttg accaggtctg acagagaggt gcagggtgaa aattgccata
    12001 ttgtcccggg atggaacgct ctattcgtgt aagcggctgc gtgaagccgc tatacagcgc
    12061 ggtcacctgg ttgaaattct tgatccgctt tcttgctata tgaacataaa tcctgcggcg
    12121 tcttctattc actacaaagg ccgcaagtta ccccattttg acgcagtgat cccgcgtatt
    12181 ggcaccgcca ttacctttta tgggacggcg gcactgcgcc agttcgagat gctggggagc
    12241 tatccgctca atgagtcggt cgccattgcc cgggcgcgtg acaaattgcg ttccatgcaa
    12301 ctgctggcgc gtcagggcat cgacctgcct gtcacgggca ttgcgcattc gccggatgat
    12361 accagcgatt taatcgacat ggtcggtggt gcgccgctgg tggtcaagct ggttgaaggc
    12421 acgcagggaa ttggcgtcgt gctggcggag acgcgtcagg cggcggaaag cgtgattgac
    12481 gctttccgcg gtctgaacgc gcatattctg gtgcaggaat atatcaaaga ggcgcaaggg
    12541 tgcgatatcc gctgtctggt tgttggcgat gaagtagtcg ctgcgattga acggcgggcg
    12601 aaagagggcg attttcgttc caatttgcat cgtggcggcg cggcaagcgt cgccagtatc
    12661 acaccacagg agcgtgaaat cgcgataaaa gccgcgcgaa cgatggcgct ggacgttgct
    12721 ggtgtggata ttctgcgtgc taatcgcggg ccgttggtga tggaagtgaa tgcgtcgcca
    12781 gggctggaag gaatagaaaa aaccaccggt atcgacatcg cgggtaaaat gatccgctgg
    12841 atcgaacgcc acgctacgac agaatattgc ctgaaaacgg gtggttagtc gcaatcacat
    12901 tactgatcat ggttttgcct gcgctttttg cgtaagctgt gccggtcttt ttatcggaag
    12961 aggttgtaca aaattatgac atcgctggtc gttcctggtc tggatacgtt gcgtcaatgg
    13021 ctcgatgacc tggggatgag tttttttgaa tgtgataact gtcaggctct gcatctgccc
    13081 catatgcaga atttcgacgg tgtctttgat gctaaaatcg atctgatcga taacacgatc
    13141 ctgttttctg ccatggcgga agtccgacct tcagccgtat tgccgctggc ggcggattta
    13201 tcagccatca atgccagttc gctgaccgtg aaagcatttc ttgatatgca ggatgataat
    13261 ctgccaaagc tggtggtttg ccagtcttta tccgttatgc agggcgtaac ctatgagcag
    13321 tttgcatggt tcgtgcgtca gagcgaagag cagatttcga tggtcattct tgaagctaat
    13381 gcccatcaac tgctgttacc gactgacgat gaagggcaaa ataacgttac cgaaaactat
    13441 ttcctccact gataacttct ttcgagcacg cagtcgctgc cgcagtgact gcgtgccgca
    13501 aaattatccg ctgattttaa ccttttatta aagattaatt cacttctctt gtgccgattt
    13561 ggctttatca catagagcaa atacgcataa aaatttgtta aataccgttt tttaatccga
    13621 gctatagtct caaaccctgg ctaaagttat tcttgcgatg cttttatata gtgagcagtg
    13681 caggccggga gagagttctc ttttcttaca ccgcgccgat aaaaatatgc acgtttattg
    13741 catatctttc agtgtgacaa cttttgttcg tttgttaacg aattttcaga aggaaagaga
    13801 tatgatcgcc ttaaataaaa aatggctatc gggtctggtt gcgggtgctc tgatggccgt
    13861 ttctgtcagc acgctcgcgg ctgaacaaaa aacactccac atttataact ggtctgatta
    13921 tatcgccccg gacacggtgg ccaactttga aaaagaaacc ggtattaaag tcgtctatga
    13981 tgttttcgat tctaacgaag tactggaagg caaattaatg gcggggagca ccggctttga
    14041 tctggtggtg ccgtcagcaa gctttcttga gcgccagctg acagcgggag ttttccagcc
    14101 gctggataaa agcaaattgc cggagtggaa gaatctcgat ccggagctgc tgaagttggt
    14161 cgccaaacac gatcccgaca ataaatttgc tatgccctat atgtgggcga cgaccgggat
    14221 tggctataac gtcgataaag ttaaagcggt gctgggcgaa aacgcgcccg tcgatagctg
    14281 ggacttgatc ctcaaacctg aaaatctgga aaaactgaaa agctgcggtg tctctttcct
    14341 ggatgcgcca gaagaagtgt ttgctaccgt gctgaattat ctcggcaaag atcctaacag
    14401 cactaaagcg gatgattaca ccggaccggc taccgatctg ctgttaaagc tgcgcccgaa
    14461 cattcgttat ttccattcat cccaatacat caatgatctg gcgaatggtg acatctgtgt
    14521 cgctattggc tgggcgggcg atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa
    14581 tggcgtgaat gtctcgttct cgattccaaa agaaggggcg atggcgttct ttgatgtatt
    14641 cgccatgcct gcggatgcca aaaacaaaga cgaagcctat cagttcctga attacctgct
    14701 gcgtcctgat gtggtggcac atatctctga ccatgtgttc tatgctaacg ccaataaagc
    14761 agccacgccg ctggtgagtg cggaagtccg tgataacccg ggtatttatc cgcctgcgga
    14821 tgttcgtgcg aagctgttca ctctgaaagt gcaggacccg aaaatcgacc gtgtgcgcac
    14881 ccgcgcctgg accaaagtga agagcggaaa ataatccgca gccgtagatg ccggaggggc
    14941 gcaccacacc cgccggcaat tcgcaccatc atggtgcgct tgcacacatt caatgccgga
    15001 gagcagccgt gaatgacgct atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc
    15061 cgctattaga aatccgtaac ctgaccaaat cctacgatgg tcaacatgcg gtggatgatg
    15121 tcagcctgac catctacaaa ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca
    15181 agtccacgct gctgcgtatg ctggcaggtt ttgaacaacc ttcggccggg cagattatgc
    15241 tcgatggcgt cgatttgtca caggtgccgc cttacctgcg ccccatcaat atgatgtttc
    15301 agtcttacgc gctgtttccg catatgaccg tggaacagaa catcgctttt ggcctgaaac
    15361 aggacaaact accgaaagcg gaaattgcca gccgggtcaa tgagatgctt gggctggtgc
    15421 atatgcagga gttcgccaaa cgcaaaccgc atcagctttc tggtggtcag cgtcagcgtg
    15481 tggccctggc cagaagcctt gccaagcgcc cgaaactatt actgctcgat gagccgatgg
    15541 gcgcgctgga taaaaagctg cgtgaccgga tgcagcttga agtggtggat attctggagc
    15601 gcgtcggtgt gacctgcgta atggttactc acgatcagga agaggcgatg actatggcgg
    15661 ggcgcatcgc cattatgaat cgtgggaaat ttgtccagat tggcgagccg gaagagatct
    15721 acgagcatcc gaccactcgc tacagtgctg aatttatcgg ttcggttaat gtctttgaag
    15781 gtgtactcaa agagcgtcag gaagatggtc tggtgcttta ttcgccgggg ctggtgcatc
    15841 cactgaaagt cgatgcggat gcttcagtgg tcgataacgt gccggtgcat gtggcgcttc
    15901 gcccggaaaa aatcatgctt tgcgaggaac cgcccgccaa tggttgtaac ttcgcggtgg
    15961 gggaggtgat acacattgcc tatctcggcg atctttcggt gtatcacgtt cgtctgaaaa
    16021 gtgggcagat gattagcgcc cagctacaaa acgcccatcg tcatcgtaaa gggttaccga
    16081 cctggggcga cgaagtgcgt ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt
    16141 aaggagcaaa gatgagtaca cttgaacctg ctgcccagcc aaaacccttt aagctgtggt
    16201 tgtcgcagct gcaaatgaaa catgggcgca aactggttat tgcgttgccg tatatctggt
    16261 tgatcttgct gtttctgctg ccatttctga tcgtctttaa aataagcctg gcggagatgg
    16321 cgcgcgctat tccaccttat accgaattga tggagtgggc tgacgggcaa ctttctatca
    16381 ctcttaatct cggtaatttt ctgcaactga ccgacgatcc gctctatttt gatgcttatc
    16441 tccagtcgtt acaggtggcg gcgatttcga ctatttgctg tttactgatc ggctatccgc
    16501 tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa tatcttatta ctactggtga
    16561 tcctgccgtc gtggacctcg tttctgattc gtgtctacgc ctggatggga atattaaaaa
    16621 acaacggtgt gctgaataat tttctgctgt ggctggggat tatcgatcaa ccgctgacca
    16681 ttctgcatac caactttgcg gtttatattg gcattgttta cgcctacgtg ccgtttatgg
    16741 tgctgccgat ttacaccgcg ttgattcgta ttgattattc gctggtagaa gcggcactgg
    16801 atctcggtgc acgaccgctg aaaacgttct ttactgtgat cgtgccgctg actaaaggtg
    16861 ggattattgc cggatcgatg ctggtgttta tcccggctgt gggcgagttt gtgatcccgg
    16921 aactgctcgg tggcccggac agcatcatga tcgggcgcgt gctgtggcag gagttcttta
    16981 acaaccgcga ctggccggtg gcctcagcgg tagcgatcgt catgttgctg ctgctaatta
    17041 tgccgataat gtggttccac aaacatcagc aaaaaagcga gggagaacac ggatgaataa
    17101 tttaccggtg gttcgttcgc cctggcggat tgtgattttg ctgttgggct tcacctttct
    17161 ctacgcgcca atgctgatgc ttgttatcta ttcgtttaac agctcgaagc tagtgacggt
    17221 gtgggccggc tggtcaacgc gctggtatgg cgaattattg cgcgatgacg cgatgatgag
    17281 tgcggttgct ttaagtctga caattgcggc ctgtgcggcg acggcggcgg cgatcctcgg
    17341 gactattgcg gcggtcgtgc tggtgcgctt tggcaggttt cgcggatcaa atggttttgc
    17401 cttcatgatc accgcaccgc tggtaatgcc agatgtcatc acgggtttgt cgctgttgtt
    17461 gttattcgtc gcgcttgctc atgctattgg ctggcctgcg gaccgcggta tgctcaccat
    17521 ctggctggcg catgtcactt tctgtacggc ttatgtggca gtcgttattt cgtcgcgtct
    17581 gcgggaactg gatcgctcga tagaagaagc cgcgatggat ctcggtgcga cgccgctgaa
    17641 agtgtttttt gtcattacgc taccgatgat catgcccgcg atcatttctg gctggttact
    17701 ggcttttact ttgtcgcttg atgatctggt gatcgccagc tttgtttccg ggccgggagc
    17761 caccacatta ccgatgctgg tcttttccag cgtgcggatg ggggtgaatc cggaaatcaa
    17821 cgccctggca acattgatcc tcggcgcggt cggaattgtc ggatttatcg cctggtatct
    17881 gatggcgcgc gcagaaaaac agcggatacg cgacatccag cgtgcaagac gtggctgaag
    17941 acactaaaat ttgccaacct ggctacataa tgccgcgcat gtcgcggcat tgttttcatg
    18001 gaagacgaaa cgttgggatt ttttaagaaa acatcttcat ctcatgctcg cctgaatgtg
    18061 cctgcgctgg tgcaggtggc ggcgctcgcc attattatga tccgtggcct cgacgtgctg
    18121 atgattttca atacgctggg cgtgcgcggt attggcgagt tcattcatcg cagcgtacaa
    18181 acctggagtt taacgctggt ctttttgagc agtctggtgc tggttttcat tgagatctgg
    18241 tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct ggctgtatct gttgacacaa
    18301 atcacagccg caagttactt gtgggcggct tcgctggggt atggttatcc ggagctgttc
    18361 agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc tgatgctgca gaagctgccg
    18421 gatatgctca tcctgatgct gctgttcgtt ccctcgacca gtcggcggtt cttccagttg
    18481 caataatgtg tataatcgtc gcccctgatg atgtgaaggt caatgtatgc agtgcgcact
    18541 ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg cagccgattc cagagcaact
    18601 ctccgctaaa actgccgatc ttaaaaatct gctcgccgat tttccggttg aggaatggtg
    18661 tgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa gccaaaatgg tggtgagtgg
    18721 tagcgttgaa aaaccactgc tcggtatgct gcatcgagat ggtacaccgg aagacctttg
    18781 tgactgcccg ctttatccag cctcatttgc gcccgttttt gcggcgctaa aaccgtttat
    18841 cgcccgtgcg gggttaacgc cctacaacgt ggcgcgtaaa cgtggtgaac tgaaatacat
    18901 tctgctgact gaaagccaga gcgatggtgg catgatgctg cgttttgtac tgcgttctga
    18961 taccaaactg gcgcaattgc gtaaggcgct gccgtggtta caggaacaat taccgcagct
    19021 gaaagttatt accgtcaata ttcagccggt acatatggcg attatggaag gggagacgga
    19081 gatctacctg accgaacaac aggcactggc agagcgtttt aatgacgtac cgctgtggat
    19141 ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc agccagctgt acgccaccgc
    19201 gcgcgactgg gtacgacagc tgccggttaa acatatgtgg gatctgttct gcggtgtggg
    19261 tggctttggt ttacactgcg cgacgcctga catgcagtta accgggatcg aaattgcacc
    19321 agaggccatt gcctgtgcga agcagtcagc cgctgaactg ggcttaacgc gtttgcaatt
    19381 tcaggcgctg gactctacgc agtttgccac cgctcagggg gaagtgccgg agctggtgct
    19441 ggttaacccg ccgcgccgcg gcatcggtaa accgctgtgt gattatctct caacgatggc
    19501 accgcgtttt attatctact ccagctgtaa cgcccaaact atggcgaaag atatccgcga
    19561 actgcctggt tatcgcatcg aacgggtaca gctttttgat atgttcccgc acaccgcgca
    19621 ctatgaagtg ctgacgctac tggtgaagca ataaaaaagc cgcaggtgcg gctttctgaa
    19681 tcttactgtg ggaaccactg gtcattgatt ttctgatatg taccgtcagc tttaattgct
    19741 gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac agcgataccc
    19801 aggccggtgc cgaagtattg cggatcggtc actttttcag tagcaacgcc cagttgcgga
    19861 ttggttttca gccattcgtt taccactgcg gtgtcaccaa atacgccatc aatacggcca
    19921 tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt cacttccggg
    19981 tgctgatcct gaatatattt ctggtgcgtg gtgccgtttt ccatcccaat acgtttgcct
    20041 ttcagatcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc tgagttttcg
    20101 tagtagggcg tggtaaacga tacctgtttg ctacgctccg gcgtgatatc cattccggag
    20161 attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa agcgtgatta
    20221 gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccaggtc gatatcaaag
    20281 ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc tgaaacgcca
    20341 aaattgattt tctctgcggc agaagcaccg aaagtaaaag aagcaagtaa agcggcaaga
    20401 actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg ccgtgtctgc
    20461 ggcatgggat aacaatgcca ttaagtgaat ttatatgcaa taaatatgag taaataattt
    20521 atatgaaata aaaaaagacg gacagcttag tgggttgtcc gtcttcataa taagaattta
    20581 tgcactatgt aggccggata aggcgtccac gccgcatccg gcatcagcat gcagctacat
    20641 taattccgcc gttcgaatgc cagtgcttta cgctcgatca gacgcatcat cagcgtcagc
    20701 aggccgttga cgaccaggta aataatcccc gccgcgccaa ataccattac atcgtaggtg
    20761 cgtccgtaca acaactggct gtatcccatc acttccatga gcgtaatggt gtaagccaga
    20821 gaggtacttt tgaacaccag caccacttcg ttggaataag aagagagcga gcgtttaaac
    20881 gcgtacggca gcaggatcgc cagcgtatct tttttgctca ttcccagggc gctacaggac
    20941 tgccattgac cttccgggat cgcgcgaatc gcaccgtaaa acagctgcgt ggtgtacgcc
    21001 gcactgttca gcgacaacgc aatcagcgca cataaccacg gttctgacaa caaatgccac
    21061 agtgccggat actcctgcaa cgtcgggaac tggcccggcc cgtaataaat caggaagatc
    21121 tgcaccagca gcggcgtacc ggtaaacagc gtgatatagc cccgtaccag ccacaccagc
    21181 accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag tgccacaatc
    21241 agcgaggcaa cggttagcgt caggctggtg tgcagccctt tcatcagttc gggtaaatac
    21301 tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg cgtttgagaa
    21361 tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg taccaggtaa
    21421 acggttcctg ggtacgggta gcgatgcttt tggtttgtag cattaaatca ttcacactaa
    21481 tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg agacccggca
    21541 gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat ttcgacagcc
    21601 ccagcgcctg accggattcc cactgcccca ccggcaccgc ttttaatgca ccacgcagcg
    21661 tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa aggaacgggc
    21721 taacgtcgaa gttctcaatg tccatctgca ccgggatctg cacgaaccca agattgatag
    21781 tgaagccatc cgaaagcgtc agcagcagct gcgaggagcc aaaatagata aacagcacca
    21841 ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc cacgcgacag
    21901 gacgccattt cgcagactcc cataccgcaa agaacatcgc cagcgccagc ccgacaatca
    21961 atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga aaaaattcat
    22021 tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg ccatctttct
    22081 tcactttttc cagcgcagtg ttgaatttct gctgcagttc agtgttgccc tggcgtactg
    22141 cgatgccgag gccagtaccg aagtaatctt tatcggtcac tttatcgccc actgccgcca
    22201 gtttcgggtt atctttcagc cattcagtta ccactgcggt gtcaccaaat acgctgtcga
    22261 tacgaccgtt ttgcagatcc agttttgcgt tctggtaact gtcatatggc acggtagtga
    22321 tttccggatg cttatccata atgaacttct ggtgtgtcgt cccgttctga acgccaactt
    22381 ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca aacagggcag
    22441 agttgtcata gtacggggtg gtaaacagca cctgcttttc acgctccgga gtgatatcca
    22501 tgcccgccat cactgcttct acgcgacgga atttcaggct tgggatcagg ctgtcaaacg
    22561 cctggttaga gaaagtgcag gttgcatcaa tctctttaca caatgcctgc gccaggtcga
    22621 catcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga taggaggctt
    22681 cggtggcaaa acgaatggtt tcggctgctg tggcggaaag actaaaacct gcaattaacg
    22741 cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga gatagttttt
    22801 aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta cgatatgacc
    22861 gttttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt cgtgggtgac
    22921 gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga tttgtgccgt
    22981 aatttcgggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg gttccatcat
    23041 caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca gcgggtaacg
    23101 atcgctataa ggtttaagac gcagacgttc cagcagtttt tctgcacggg ccagcgcctg
    23161 atctttactc agccccagta cacggcaggg cgcttcaatc aggttttgct gcacggtcag
    23221 atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca aatcgcgaat
    23281 cgctttgtca gagggggttt tggtgaaatc gaaatggttg cctgcaatgt tgagcgtacc
    23341 ggagcgcggc atctcaagca gattgagtac acgcagcagc gagcttttac ccgcgccgct
    23401 ggggccaagt aacaccagcg tttcgccctg cgggcaatcc agcgtgatat cgaacagcgc
    23461 ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg acactcgtat
    23521 actggcagtc tgatagctat tgaggtcgaa gatagtacct ttgacagaat aattatgcaa
    23581 tatttctgct ttaaaagtta aaagcaaagc gcattattca ataaacatag cacaaaataa
    23641 cgggggcggt ggtcggcgag cataaatgtc ggcattcctc acgaaatgcc ggacaattta
    23701 cggggtttat tggttgatca agacgttagc gattctcgat ggattgacgg agcgtacccg
    23761 ccgtggcatg aacgctaccg cctaagtagc gcacatcgtc gatgacccaa cactggcctt
    23821 cctgaatcat taacacttca tcctgccagc cctggtcacc ctgcttgaga tccacgcgca
    23881 atggaatgtt acgggcatca cgattaggga tagtcgatgc actggctacg tgggcgctgt
    23941 ctggcaaggt ggttcgactg gagaacggat cgctgcttaa caattcacga tgactattat
    24001 cgcggctggc atcgctaagt agggttgcca gtttgtcact caaatagggg cgcagagcgg
    24061 taatgtcatt gctgcgatgt tgaatgcggt agtcatagaa ctgttgagcc acactatcag
    24121 gccccccctg gacacaggaa ccggtgcggg ggccgttatc tttataagct ggcgtgacgg
    24181 tggtacaggc actgagtagc agtgcgcaag ggataagcat ggtcaatttg ctatagcgca
    24241 tgatgatttc cttataagcg aacaccttga aagcgttcta cgataataat gatatccttt
    24301 caataatagc gtatcagtcc gataatgctt tgaagatcga aggcttagca aacaaggaga
    24361 tcgatcatgc aattttctac aaccccaact ctggaaggcc agaccatcgt tgaatattgc
    24421 ggtgtggtga ctggcgaagc gattttaggc gcgaatattt tccgtgattt ctttgccggt
    24481 atccgcgata tcgttggcgg acgctccggt gcctacgaaa aagaactgcg taaagcgcgg
    24541 gagattgcct ttgaggagct cggctcccag gcgcgagcgc tgggggctga tgccgtcgtc
    24601 ggtattgata tcgactacga aacggtcggg caaaacggca gtatgctgat ggttagcgtc
    24661 agcggtacgg cggtgaaaac gcgtcgatga gaagagtctt ctggctggtc gctgccgctc
    24721 tgttactggc agggtgcaca ggcgaaaaag gcattgtcga gaaagaggga tatcagcttg
    24781 atacccgacg ccaggcgcag gcggcgtatc cgcgcattaa agtgctggtg atccactaca
    24841 ccgcagatga ttttgatagc tcgctggcga cactgaccga taagcaggtc agctcgcatt
    24901 atctggtccc tgcggtgcca ccgcgataca acggtaaacc gcgcatctgg caactggtgc
    24961 cggaacaaga actggcctgg catgcgggga ttagcgcctg gcgcggggca acgcgtctta
    25021 acgacacctc tattggcatt gagctggaaa accgtggctg gcaaaaatcg gcgggagtga
    25081 aatattttgc cccgtttgaa ccggcacaga ttcaggcgct tattccgctg gcgaaagata
    25141 ttattgcccg ttatcacatc aagccggaaa acgtagtagc acatgcggat attgcgccac
    25201 agcgcaaaga cgatccgggg ccattatttc cctggcagca actggcacag caggggattg
    25261 gtgcctggcc ggatgcgcag cgggtgaact tttaccttgc cgggcgcgcg ccgcacacac
    25321 cggtagagac cgcgtcattg ctggagcttt tggcgcgtta tggttatgac gtaaagcctg
    25381 atatgactcc gcgcgagcag cggcgtgtga ttatggcgtt ccagatgcat ttccgcccga
    25441 cgttatataa cggcgaagcg gatgcagaaa ctcaggcgat tgccgaagcg ttgctggaga
    25501 aatacgggca ggattagcgc ggcagttttc cgtggtcgcg cagccaggcg gcagttttct
    25561 cgataccttc atccagggtg aggactggct gataacctaa ctcttcctgc gcacgcgtaa
    25621 tatccagcgt aaagtcaaaa ttcaacttgg agacgccgta gtgggtcagc ggcggctctt
    25681 ttgctgactt gcggcctaaa cgctccatgc tgcgggcgat catatccagc atcgggtaag
    25741 gaaccgaacg aatacgacag tcaatattca actcgtcgat cagcttctgc acgatgctgc
    25801 gcagtgtgcg atgctcgccg ttggtgatgt tgtacacacg cccggaaggt agcttatcgc
    25861 acgcttcctg gcttgccagc cacatggcgt gcacggcatt ttcatagtag gtcatatcga
    25921 ccagcgcact gccgccatgc ggtaacagaa tactgccgta gtgatgcatc atatgcgcca
    25981 gacgagggat aaagacttta tcgtgcggtc cgaacagact ttgtgggcgc agaatagtaa
    26041 agcgcgtttg tggattcgcc tgcgaaagca tattgatcac ttcttcgctg gctgctttgc
    26101 tgcgggcaaa ctcgttggcg aagcggtgag ggcgaaaatc ttctttaata tcgcgatggt
    26161 ggtgataatc gaagtacagg gagggagaag agatatgaat aaagttacgc acaccccagg
    26221 cgacagccca ttcacccagg cggcgagtgg cgcgaacgtt agccagatcg aaagcctgtt
    26281 gtgtccccca gggcgaggta aagctggagc agtgccacag cgtatcaatg cccgcgagca
    26341 tcactttagc ttgtgatgag accaactcgg tcagatccgc cggaacaaac tctgcgccca
    26401 ttttttccag caatttaccc attgcctcgt tgcgaccggt cgctcgcacg ctgatgcctt
    26461 tctggcataa aaactctacc gcgtttcgac ctaagccgct ggtggcgccg gtaaccagta
    26521 ccttcatatc aatccactgt tgttgagaaa atgacgtgcg cattcttccg tgatttcccc
    26581 cttgatgcaa tgggaaacat gaaagaataa cgcaggtttt gtcgattaat ctgtgctttg
    26641 ttctgccagt ctggcgattc gttttgccat tccgcgaaaa ataaacagat gcgcggggat
    26701 catcaataac cagtaaaaca gccccggcat accgtgcgga tgccagaaag cacggacatc
    26761 gattgtacga tagtcgcctt tatcttccag ggtaaaacag agtcgtccca gccccggcgc
    26821 tttcatgcca aataacaacg taagttgttt ttccggttca acgacaatga ctttccagct
    26881 atccacagca tcgccggtct gtaaatattc gcgctccggg cggccttttg ccagcttatg
    26941 accgatcgca aggtccatca acgcccgtgt ctgccacaaa atattgccaa agaaataacg
    27001 ctctttaccg ccgatttgat tgaccacccg ccatagcgcc tgaaggctgg cagacgtttt
    27061 aacggtaaac cccgcctgtt tggcaaaata accgtattct ggtcgccagc gagcaaaagc
    27121 ctgcgcgtca tatccccagt cgctggagtt gaccagtttt tcttcctctt tcaacgtgct
    27181 acgtaccgcg tcatcgaaag cgatcagcgg ttgtggaatg agtgcacgta acgcggtgtc
    27241 atccgccagc agatcgtgtt tcagcccctg aatcaacgct ctggcggtgg tgggcggtac
    27301 tgaagtaatc acattaagaa accacaccga aatccagcgg gtggggaggg ggatggggag
    27361 caaccagcgg cgcttaccgc tcaccgccat aaaatgttca aactgttgct gataactgag
    27421 cacttctggc ccagcggctt cgaagatgcg atgttcgcaa gccggatgat ctaacagcgc
    27481 cacaagatag tgcagcaggt tttccagcgc gatgggcgta gtgcgtgaac gtacccagcg
    27541 tggtggcgtt aatactggca ggttgtaaac catatcgcgc atgacttcga atgccgctga
    27601 acccgcgcca acgataattc cggcacgtag ttcggtcacc gggacacccg cttcacgtaa
    27661 aatatccgcc gtggcctgac gagcacgcag atgatcagat tgttcatgag gcggtgcctg
    27721 caatgaactg agaaagatga cttgctttac cggtacttcg cgcagcgcat cgcggacgtt
    27781 gagagccacc tggcgctcct gagcgataaa atcaccacct tcgcccatgc tgtgcaccag
    27841 aaaatagaca gtatcaatat tctgcaacag gaccggaagg ttatccggcc agttgagatc
    27901 gactttatgg caactgacat tagccagttg cagttttgca agtctgtcga cgtgacgtgc
    27961 cgccgccagg atctgatgcc cttgctggct gagtgtgtgc accagatgtt gaccaatgta
    28021 gccactggca ccgagaacta aaatgcgttg cggcacgtct ctctccttaa cgcgccagga
    28081 acgcacgcca gtgggcggca acttccgcca gttgttcacg cgagacgtca agatgggtca
    28141 ccaggcggac aatcggcgag gcgttaatca gcatgtttct cgctttcatg tattcgccta
    28201 acgcggcagc attttcttcc ccgacgcgaa caaacagcat attggtgtcc tgacgcatca
    28261 catccgcgcc tgcttcacgc aactgctccg ccatccaggc ggcgttatcg tggtcttcct
    28321 gcaaacgtgc gacgttattt ttcagcgcat acatcccggc ggcagccaga atgccggact
    28381 gacgcatccc gccacctgtc attttccgcc agcgaatggc acgtttaatg taatcacgat
    28441 taccgacgag taatgaaccg actggcgtcc caagaccttt cgacaggcaa atggtgaacg
    28501 agtcacaata ttgcgtgatc tctttcagtt cgcagccgta agccaccacg gcattaaaaa
    28561 tgcgcgcacc gtcaacgtgc agcgccagat tgcgctcgcg ggtaaattcc catgcttctt
    28621 tcaggtattc acgcggcagc actttgccgt tgtgggtgtt ttccagactg agtaatttgg
    28681 tgcgggcgaa atggatatcg tcgggtttga ttttcatcgc cactttatcc agcggtagcg
    28741 taccgtcggc agctgcgtcg atgggttgcg gctgaatact gcccagtact gctgcgccac
    28801 cggcttcaaa cagatagtta tgcgcggcct gaccgacgat atactcttcg ccgcgttcgc
    28861 agtgactgag cagagcgacc aggttggcct gagtgccggt aggcagaaaa atggcggctt
    28921 ctttaccgga aagctctgct gcatagtcct gcagagcatt aacggtaggg tcgtctccat
    28981 aaacgtcgtc cccaaccggg gcggccatca tcgcttcgag catggcgcgg ctcggtcggg
    29041 taacggtatc actgcgtaaa tcaatcatgg catgtcctta ttatgacggg aaatgccacc
    29101 ctttttacct tagccagttc gttttcgcca gttcgatcac ttcatcaccg cgcccgctga
    29161 tgattgcgcg cagcatatac aggctgaaac ctttggcctg ttcgagtttg atctgcggtg
    29221 gaatggctaa ttcttctttg gcgaccacca catccaccaa caccggaccg tcgatggaga
    29281 aggcgcgttg cagggcttca tcgatttcag acgctttttc tacgcggata cccgtaatgc
    29341 cgcaggcttc ggcaattcgg gcaaagttgg tgtcgtgtag ttcggtgccg tcagtcaaat
    29401 agccaccagc tttcatctcc atcgccacaa agcccagcac gctgttatta aagacgataa
    29461 ttttcactgg cagcttcatt tgcactaccg agaggaaatc gcccatcaac atgctaaaac
    29521 cgccatcgcc gcacatggcg accacctggc gttctggctc ggttgcctgc gcgcccagcg
    29581 cctgcggcat ggcgttagcc atcgaaccat ggttaaacga acctaacagg cgacgcttgc
    29641 cgttcatttt tagataacgt gccgcccaca ccgttggcgt accaacgtca caggtgaaaa
    29701 tagcgtcatc ggcggcaaaa tgactaattt gctgcgccag atattgcggg tgaatggctt
    29761 tctcgctcgg tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg
    29821 ccttatccag aaacttgcgt tcggtttttt cttccaccag tggcagcaat gcgcgcaggg
    29881 ttgatttgat atcgccgacc agcgccatat ctaccttgct atgcgcgccg atgctggctg
    29941 ggttgatatc aatctgaata attttggcat cggtcgggta gaacacacgg tagggaaact
    30001 gagtaccgag cagcactaac gtatcggcgt tcatcatggt atggaaacct gacgaaaagc
    30061 cgattaatcc cgtcattcca acatcatatg gattatcgta ttcgacatgt tctttaccgc
    30121 gcagggcatg gactataggc gctttaattt tcccggcaaa ctcaactaac tctttatgcg
    30181 cccccgcaca gccgctgcca cacatcaggg cgatattgct ggaataacgc agcagttgcg
    30241 ccagtttgcg taactcttct tcttccggtg ttactaccgg ctgtggcgca tgataccagt
    30301 gggtagttgc cccttctggc gcaggtttta acgccacatc gcccggtaac acaacaacgg
    30361 aaacgccacg gttaagcacc gctttgcgca tagcaattgc cagcacttgt gggatctgct
    30421 ctgggctgga aaccagctcg caatagtgac tacattcgcg gaatagctct tgtgggtggg
    30481 tttcctggaa atagccgctg ccaatttcgc tggagggaat atgagcggca atcgccagta
    30541 ccggaacgtg attgcggtgg caatcgaaca ggccgttgat taagtgcagg ttgccgggac
    30601 cgcacgatcc ggcacagacc gccagttctc cgctaagttg tgcttcagcg ccagcggcaa
    30661 atgccgccac ttcttcatgc cgggtggaca tccactcgat ggtgcccatg cgattaagac
    30721 tgtcactaag accgttcaga gagtcgcccg tgactcccca gatgcgtttc acgcctgccg
    30781 attcgagtgt tttggcgata taagctgcaa ccgtttgttt catggttctc catctcctga
    30841 atgtgataac ggtaacaagt ttagttcatc tgacggaggg ggaagggatg ggagaaaaag
    30901 gaggcactaa cggttaaata gcccgatgaa aggaatatca tcgggcatca ggcgattatg
    30961 cgaaaaccaa atccccctgc ggatggcatg agcaggccag tacgtaacct tcagcgattt
    31021 cggcgtcggt cagcgtcatt gtgctgctca ccgtatattc accggaaatc actttcgtct
    31081 tacagcagcc gcaaacaccc gcacggcagg cggcgacaac cggaacgtta ttgctttcca
    31141 gcgcctccag tagcgtggtg ccaaccggag cgtaaaattc tcgtgccggt tgcagtttgg
    31201 tgaatttcag accgctggtc gccgcttccg ctactggggt gaagaatttc tctttaaaga
    31261 aacgcgtcac gccgagcgtt ttcacttcct gctctaccca atccatatat ggagcagggc
    31321 cacaggtcat cacggtacgt gaggctaaat cagggacacc tgccagcagt tcgcgagtga
    31381 gacgaccagc gataaagcct tcggtaacgt tattttccgc caccagcgtt accggatagt
    31441 tacgccactc atcggcgaaa atcacatcct gcggcgtacg cacgttgtag atcacctgca
    31501 catcggcctg tggacggttc ttcgccagcc agcgacgcat cgacataatc ggcgtgacgc
    31561 cgcagcctgc cgccagcaac aggaatttat cttctgcttt atcgtcgcag gtaaattccc
    31621 ccatcgcgtc cgaaagccag agataatcac cgcgttttac atcgcgcgtc agccaccggg
    31681 agccgacacc gtcatcaatc cgccgcacag tcagggtgat gtattcactc acgcctggcg
    31741 tggaggaaat ggtgtaagca cgcagcgttt ctgctgagtt tcgcacgctg accagcgcat
    31801 attgcccggc gcgatacggg tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg
    31861 gcgtttcttg cgtaatgtga tgaacctgca tccgccacgg gcattgattc gttggcatcg
    31921 tcatcgacaa actccttacg cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat
    31981 agaacgcagg ccgaatttct cgttcagcac cgccagcagg tctggtgtca ggaaaccagg
    32041 tgcagtcggg ccggtgacga tatttttcac acccagagaa agcagcgtca gcagaatgac
    32101 aatcgctttc tgttcaaacc aggagagcac cagcgacagc ggcagatcgt tgactccgca
    32161 gcccagtttc tctgccagcg ttaccgccag aataattgct gagtaagcat cgttacactg
    32221 accagcatct accagacgcg gcagaccttc gatatcgcca aactcaagtt tattaaagcg
    32281 atatttaccg caagcgaggg tcaggatcag gcagtcatcc ggcacgctgg tggcgaaatc
    32341 ggtgaagtag tgacgctcgc cgcgtgcgcc gtcacagccg ccaagcagga agatatgacg
    32401 cagtttttca cggctcacca gatcaatcag cgtatcggct gcgccaagca gtgtctggcg
    32461 accaaagccc acggtgatca ggtgcggaat ttcgctgtac gggaagcccg ccatctgttg
    32521 cgcctgggca atgaccgcag agaaatcttc gccatccaga tggcgcacac ccggccagcc
    32581 aacgatgctg cgggtccaga tacgatcgtc ataagcgcct acggttgggt cgatgatgca
    32641 gttcgaggtc atcacgatgg ggcctgggaa acgagcgaac tccacttgct gattctgcca
    32701 gccgctgccg tagttaccga ccagatgctt gaatttacgc agctccgggt aaccatgcgc
    32761 aggcagcatt tcgccgtggg tgtagacatt aacgcccgtg ccttcggtct gttccagcag
    32821 gttgtagaga tctttgagat cgtgaccgga aatcaggatg catttacctg ccgtcgcttt
    32881 gacattgacc tgggttgggg tcgggtgacc gtatttaccg gtttcgcctg cgtccagaat
    32941 gctcatcact ttgaagttca tctggccgat ttccattgaa cactcaagaa gcgcgttcat
    33001 atcggcaggc caggtcccca gccacgccat gattttatgg tactgggcat aaatatcgtt
    33061 gtcgtattga ccgagaacat gcgcgtgttc catataggcc gccgcacctt tcaggccata
    33121 caggcacagc agacgcaggc cgagaatgtt ttcgccaatc gccgctttat ctttgttagg
    33181 ggtaaattct gctgcctgac gttgcagctc gccgagatcg tcgctcacca gttgcaggtc
    33241 agccatcggg ttatcgacgc gggcgttggc atctacagcc aggcattgtg ctttcagcgc
    33301 ctcgcgcagg gcaatcgctt cacgagcgta gccgataata cgcggagaat cgaagttaac
    33361 gttggtcagg gttgagaaaa aggcacgtgg cgcaaagctg tctacatcgt ggttgatgat
    33421 gccgtattca cgtgctttca ctgcccaggc cgaaagccct tgcagcgtcg cgatgagtaa
    33481 atcctgaagg tcagaggttt ccgccgtttt accacacatc ccctgcgcgt atgagcagcc
    33541 g
//
LOCUS       DAJOTB010000036        33310 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJOTB010000036 DAJOTB010000000
VERSION     DAJOTB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.036"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            77..640
                     /gene="srlA"
                     /locus_tag="OHN28_001737"
                     /gene_synonym="gutA"
     CDS             77..640
                     /gene="srlA"
                     /locus_tag="OHN28_001737"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCQ2297144.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            637..1596
                     /gene="srlE"
                     /locus_tag="OHN28_001738"
                     /gene_synonym="gutE"
     CDS             637..1596
                     /gene="srlE"
                     /locus_tag="OHN28_001738"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCQ2297145.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1607..1978
                     /gene="srlB"
                     /locus_tag="OHN28_001739"
                     /gene_synonym="gutB"
     CDS             1607..1978
                     /gene="srlB"
                     /locus_tag="OHN28_001739"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCQ2297146.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1982..2761
                     /gene="srlD"
                     /locus_tag="OHN28_001740"
     CDS             1982..2761
                     /gene="srlD"
                     /locus_tag="OHN28_001740"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCQ2297147.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2867..3226
                     /gene="gutM"
                     /locus_tag="OHN28_001741"
     CDS             2867..3226
                     /gene="gutM"
                     /locus_tag="OHN28_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCQ2297148.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3293..4066
                     /gene="srlR"
                     /locus_tag="OHN28_001742"
                     /gene_synonym="gutR"
     CDS             3293..4066
                     /gene="srlR"
                     /locus_tag="OHN28_001742"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCQ2297149.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4059..5024
                     /gene="gutQ"
                     /locus_tag="OHN28_001743"
     CDS             4059..5024
                     /gene="gutQ"
                     /locus_tag="OHN28_001743"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCQ2297150.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5021..6535)
                     /gene="norR"
                     /locus_tag="OHN28_001744"
     CDS             complement(5021..6535)
                     /gene="norR"
                     /locus_tag="OHN28_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCQ2297151.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6722..8161
                     /gene="norV"
                     /locus_tag="OHN28_001745"
     CDS             6722..8161
                     /gene="norV"
                     /locus_tag="OHN28_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCQ2297152.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8158..9291
                     /gene="norW"
                     /locus_tag="OHN28_001746"
     CDS             8158..9291
                     /gene="norW"
                     /locus_tag="OHN28_001746"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCQ2297153.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(9513..11765)
                     /gene="hypF"
                     /locus_tag="OHN28_001747"
     CDS             complement(9513..11765)
                     /gene="hypF"
                     /locus_tag="OHN28_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCQ2297154.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11918..12445)
                     /gene="hydN"
                     /locus_tag="OHN28_001748"
     CDS             complement(11918..12445)
                     /gene="hydN"
                     /locus_tag="OHN28_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCQ2297155.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            12873..13310
                     /locus_tag="OHN28_001749"
     CDS             12873..13310
                     /locus_tag="OHN28_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297156.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
     gene            complement(13402..13821)
                     /locus_tag="OHN28_001750"
     CDS             complement(13402..13821)
                     /locus_tag="OHN28_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2297157.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(13847..14032)
                     /locus_tag="OHN28_001751"
     CDS             complement(13847..14032)
                     /locus_tag="OHN28_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297158.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            complement(14057..14524)
                     /gene="hycI"
                     /locus_tag="OHN28_001752"
     CDS             complement(14057..14524)
                     /gene="hycI"
                     /locus_tag="OHN28_001752"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCQ2297159.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(14517..14927)
                     /gene="hycH"
                     /locus_tag="OHN28_001753"
     CDS             complement(14517..14927)
                     /gene="hycH"
                     /locus_tag="OHN28_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCQ2297160.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(14924..15691)
                     /gene="hycG"
                     /locus_tag="OHN28_001754"
     CDS             complement(14924..15691)
                     /gene="hycG"
                     /locus_tag="OHN28_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCQ2297161.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(15691..16233)
                     /gene="hycF"
                     /locus_tag="OHN28_001755"
     CDS             complement(15691..16233)
                     /gene="hycF"
                     /locus_tag="OHN28_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCQ2297162.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(16243..17952)
                     /gene="hycE"
                     /locus_tag="OHN28_001756"
     CDS             complement(16243..17952)
                     /gene="hycE"
                     /locus_tag="OHN28_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCQ2297163.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(17970..18893)
                     /gene="hycD"
                     /locus_tag="OHN28_001757"
     CDS             complement(17970..18893)
                     /gene="hycD"
                     /locus_tag="OHN28_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCQ2297164.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGRVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(18896..20722)
                     /gene="hycC"
                     /locus_tag="OHN28_001758"
     CDS             complement(18896..20722)
                     /gene="hycC"
                     /locus_tag="OHN28_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCQ2297165.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(20719..21330)
                     /gene="hycB"
                     /locus_tag="OHN28_001759"
     CDS             complement(20719..21330)
                     /gene="hycB"
                     /locus_tag="OHN28_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCQ2297166.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(21455..21916)
                     /gene="hycA"
                     /locus_tag="OHN28_001760"
     CDS             complement(21455..21916)
                     /gene="hycA"
                     /locus_tag="OHN28_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCQ2297167.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            22128..22478
                     /gene="hypA"
                     /locus_tag="OHN28_001761"
     CDS             22128..22478
                     /gene="hypA"
                     /locus_tag="OHN28_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2297168.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            22482..23354
                     /gene="hypB"
                     /locus_tag="OHN28_001762"
     CDS             22482..23354
                     /gene="hypB"
                     /locus_tag="OHN28_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCQ2297169.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            23345..23617
                     /gene="hypC"
                     /locus_tag="OHN28_001763"
     CDS             23345..23617
                     /gene="hypC"
                     /locus_tag="OHN28_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCQ2297170.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            23617..24738
                     /gene="hypD"
                     /locus_tag="OHN28_001764"
     CDS             23617..24738
                     /gene="hypD"
                     /locus_tag="OHN28_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCQ2297171.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            24735..25745
                     /gene="hypE"
                     /locus_tag="OHN28_001765"
     CDS             24735..25745
                     /gene="hypE"
                     /locus_tag="OHN28_001765"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCQ2297172.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            25819..27897
                     /gene="flhA"
                     /locus_tag="OHN28_001766"
     CDS             25819..27897
                     /gene="flhA"
                     /locus_tag="OHN28_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCQ2297173.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            27942..28370
                     /locus_tag="OHN28_001767"
     CDS             27942..28370
                     /locus_tag="OHN28_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HCQ2297174.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(28409..28753)
                     /locus_tag="OHN28_001768"
     CDS             complement(28409..28753)
                     /locus_tag="OHN28_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCQ2297175.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            29039..31600
                     /gene="mutS"
                     /locus_tag="OHN28_001769"
     CDS             29039..31600
                     /gene="mutS"
                     /locus_tag="OHN28_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCQ2297176.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            31706..32362
                     /gene="pphB"
                     /locus_tag="OHN28_001770"
     CDS             31706..32362
                     /gene="pphB"
                     /locus_tag="OHN28_001770"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2297177.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(32413..33180)
                     /locus_tag="OHN28_001771"
     CDS             complement(32413..33180)
                     /locus_tag="OHN28_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2297178.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
BASE COUNT         8041 a         9046 c         8758 g         7465 t
ORIGIN      
        1 cacttttaaa tctttcaatc tgattagatt agattgccgt ttggtaataa aacaataaat
       61 actgaaggag agaacaatga tagaaaccat tactcatggc gcagagtggt tcatcgggct
      121 gttccagaaa ggcggagaag tgtttaccgg gatggtaacc ggcattcttc cgctgttaat
      181 tagcctgctg gttatcatga acgcattgat taattttatc ggtcagcatc gtattgaacg
      241 atttgctcaa cgttgcgccg gtaaccctgt ttcccgttat ctgctgctac cgtgcattgg
      301 tacgtttgtc ttttgtaacc cgatgaccct aagcctgggt cgctttatgc cggaaaagta
      361 caaacccagc tattacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc
      421 ccatatcaac cctggcgaac tgtttgttta tctcggcatt gccagcggtc tgacgacgct
      481 gaatctgcca cttggcccac tggcggtgag ctatctgctg gttggtctgg tcaccaactt
      541 cttccgcggc tgggtaaccg atctgaccac cgccattttc gagaaaaaga tgggcattca
      601 acttgaacaa aaagttcgtc tggcaggagc aacatcatga cacgcattcg gatcgaaaaa
      661 ggaacgggtg gctggggcgg cccgcttgag ctggaagcca cgccgggcaa aaaaatcgtc
      721 tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct tactggctgg
      781 caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta
      841 atcgactgtg gcggcacatt acgctgcggc atctatccga aacggcgtat tcccaccatt
      901 aatatccact cgacgggcaa gtctggcccg ctggcgcagt acattgtgga agatatttat
      961 gtctctggcg taaaagaaga aaacatcact gtagtgggtg aggcgacacc acaaccctct
     1021 tccgtgggcc gtgactatga caccagcaag aaaatcaccg aacaaagcga tggtttactg
     1081 gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt tgtttcaatc tggtcgtgac
     1141 accatcgaca ctgtattaaa aaccattctg ccgtttatgg cgttcgtctc ggcgcttatc
     1201 ggcatcatta tggcttccgg tcttggcgac tggattgccc acggtcttgc tccgctggcg
     1261 agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccgct gctttcacct
     1321 ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt
     1381 ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag
     1441 gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagctcgtca ggacacggtt
     1501 cgcgtcggtg tcccttctgt tctggtgagc cgctttttaa ccggcgcgcc aactgtactg
     1561 atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta
     1621 tcagaccacc atcacccgta ttggcgcgag tgccactgac gccctcagcg accagatgct
     1681 catcaccttt cgtgaaggcg cgccagcgga tctcgaagag tattgcttca ttcattgcca
     1741 cggcgagttg aaaggtgcac tccatcccgg tttgcaattt acgctcgggc ggcatcgcta
     1801 tccggtgacc gccgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac
     1861 cctgcgcttc gatggtttaa gcgaagcgga atttccgggc actgtccatg tggcaggccc
     1921 tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa
     1981 aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca
     2041 cggtctggct gccgaggggt atcgcgtggc ggttgtcgat attcagagcg acaaagccgc
     2101 aaatgtggca caagaaatta acgccgaata tggtgaagga acagcgtacg gttttggtgc
     2161 tgacgccacc agcgagcaaa gcgttctggc gctttctcgt ggggtagatg aaatctttgg
     2221 tcgcgtggat ttgctggtct acagcgccgg aatagcaaaa gcagccttta tcagcgattt
     2281 ccagcttgga gattttgacc gttcgctaca ggtgaatctg gtgggttatt tcctgtgtgc
     2341 ccgtgaattt tcgcgtttga tgattcgcga cgggattcag ggtcgcatta ttcagatcaa
     2401 ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg
     2461 tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca
     2521 ttcgctgatg ctcggtaacc tgctgaaatc gcctatgttc cagtcactgt tgccacaata
     2581 cgcgaccaag ctgggtatca aaccggagca agtcgagcag tattacatcg ataaagtacc
     2641 gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg ctgctgttct acgccagccc
     2701 taaggcgtcg tactgcactg gtcagtcgat caatgtcacc ggcggtcagg tgatgttctg
     2761 atcaacagcg gagatctttt aaggatctcc gcgcgactaa tagaatgcct gatgcgctac
     2821 gcttatcagg cctacaggac ttccgccact acatcacgga gaagttatgg tatccgcact
     2881 catcaccgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggtt ggcaaatttc
     2941 tcgttttaac cgtgccttcg acacactatg ccaacaaggg cgcgttggcg tgggacgttc
     3001 cagcgggcgc tttaaaccac gggtcgtggt cgccatcgcg ctggacgatc agcagcgcat
     3061 cgtcgacacc ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc
     3121 aattaccggc atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact
     3181 atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg
     3241 ttactagctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc
     3301 tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga
     3361 agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct
     3421 ggaacatgcc ggaaccgtca ttcgtactta tggcggcgtg gtgttgaaca aagaggaatc
     3481 cgatccgcct atcgatcata aaacgcttat caacacccac aagaaagagc tgattgcaga
     3541 agctgccgtt ggttttattc atgatggcga ctcaataatt cttgatgctg gcagtaccgt
     3601 gttgcagatg gtgcctctgc tctcacgttt taataacatc acggtgatga ccaacagtct
     3661 gcacatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg
     3721 aacatttcgt aaaaaatcgg cctcatttca cgggcaactg gcagagaatg ccttcgagca
     3781 tttcaccttc gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac
     3841 cacgttcaac gaggtttata ccgtcagtaa ggcgatgtgc aatgccgcgc gcgaagtgat
     3901 tttgatggct gactcatcga agtttggtcg taaaagcccc aacgtggttt gtagtcttga
     3961 aagcgtcgat aagctgatta ccgacgcagg tatcgatcca gcgtttcgtc aggcgctgga
     4021 agaaaaaggg atcgacgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg
     4081 cgggacgtca gacgttaatg ctggagttgc aggaagcaag tcatttaccg gaacgtctgg
     4141 gcgatgattt cgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt
     4201 cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacgctt gccagcaccg
     4261 gcacaccggc tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg
     4321 aaagccgcga tgtgatgctg tttatctctt attccggtgg cgcgaaggaa ctggatctca
     4381 ttattccgcg tctggaagac aaatctatcg cgctgctggc aatgaccgga aaaccgacgt
     4441 caccgctggg cctggcggcg aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc
     4501 cgatgcacct tgcgccgacc tccagcaccg tgaataccct gatgatgggc gacgcgctgg
     4561 cgatggcagt catgcaggcg cgtggtttta atgaagaaga ttttgcacgc tcccacccag
     4621 ccggggcact gggcgctcgc ttgctgaaca aagtgcatca tcttatgcgt cgtgacgatg
     4681 ccatcccgca ggtagcgtta accgccagcg tgatggacgc gatgctggaa cttagccgca
     4741 ccggtttagg tctggtggca gtgtgcgatg atcaacgact ggtgaaaggc gtctttaccg
     4801 acggtgattt acgtcgctgg ctggttggcg gcggcgcact caccacgcca gtcaatgaag
     4861 cgatgacggt cgacggcacc acgttgcaat cgcaaagccg tgccatcgac gccaaagaaa
     4921 tcctgatgaa acgaaaaatc accgccgcac cggtagtgga tgaaaacggc aaactcaccg
     4981 gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc agccccagac
     5041 gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgca caggcggccc
     5101 agttgtgatg attttgcgcc agtgcctgac gaatggtttc acgctggaac gcttctgtcg
     5161 cttcacgcag attttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca
     5221 cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgctg cgggtggctc
     5281 tcgacaatac taccgcccga tgaatagcat gttccagttc gcgcacgttt cccggaaaat
     5341 tgtagtgttg cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc
     5401 gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac
     5461 gcagcggcgg cactgaaagt ggaaacacgc tcaggcggtg gaacaagtca gcgcgaaatc
     5521 gccctgccag tacctcttcg cgtaagtcac ggttagtcgc cgccagcacg cgcacatcga
     5581 cccgcaaact gcggtcatcg ccaacgcgtt gaatatcgcc atactgcaac accctcagca
     5641 gcttggcctg caatgccaac gacaactcgc cgatctcatc gagaaacagc gtgccgttat
     5701 ccgccatttc aaacttcccg ctgcggttac tgatagcacc agtaaacgcc cctttcacat
     5761 gcccgaacaa ctcactttcc gccacacttt ccggcagtgc ggcacagttg agatagacca
     5821 gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg
     5881 ttcccgtctc accgctaatt aaaacgttga gatcggatgc cgccacaatc tcaatctctt
     5941 ttttcaattg cgtcatgcct ggggacaaac caatcatctg cgtctgtttc accgcttcaa
     6001 acggcgcggc atcgcctggc agcatattct ggctttccag ttgttcaatc agtaacgcat
     6061 tgcttaacgc tcccgccgcc agcgcagcaa tcaggcgtaa ctcttcgtcg ctgaaaacat
     6121 cgaactgatc gggctgcatc ccgtcgagcg tcaaggcacc gatcaggttt tgcccggcaa
     6181 acaacggcaa accaacgcag gcgtgaacct tcagactctc ctgccctgga atcagaccgt
     6241 cataaggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatcaccc gcgcgggcaa
     6301 tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca tccttcgcca
     6361 gcccgtcgat ggcaagcgga ataaactgcc gcgagtcgta acgtagcaac gcagacgcat
     6421 cgcactccag cacctgacgc agcgtagtga tcaggcgctg aaaacgatcc tgatgaccaa
     6481 tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg
     6541 cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt
     6601 gactactcat taatgagcat aattttattt atagagtaaa aacaattaga taaaaaactg
     6661 gcacgcaatc tgcaatcagc aagacatctt tttagaacac gctgaataaa ttgaggttgc
     6721 tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg
     6781 tgattttcac ggtacagaat ataaaacgct gcgcggcagc agctacaaca gctacctcat
     6841 ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt
     6901 gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaatcatgc
     6961 agaagaagac cacgccggag cgctgaccga actgatggcg caaattcccg atacgccgat
     7021 ctactgtaca gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa
     7081 ttttaatgta gtgaaaactg gcgacacact ggatatcggc aacggcaaac aactcatttt
     7141 tgtcgaaacg ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc
     7201 ggtgctgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga
     7261 tgaagtggat cagactgagc ttttcgagca gtgccagcgt tactacgcca atatcctgac
     7321 gccgttcagc cgcctggtaa cgccgaaaat taccgagatc ctgggcttta acttgccagt
     7381 cgatatgata gcgacctctc acggcgtggt atggcgcgat aacccgacgc aaattgtcga
     7441 gctgtacctg aaatgggcgg cggattatca ggaagacaga atcaccattt tctacgacac
     7501 catgtcgaat aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga
     7561 cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac
     7621 caatgtcttc cgctcaaaag gcgtactggt cggcacttct acgatgaata atgtgatgat
     7681 gccgaaaatc gccgggctgg tggaggagat gaccggatta cgcttccgta acaaacgcgc
     7741 cagcgctttc ggctctcacg gctggagtgg cggtgcggtg gatcgtcttt ccacgcgcct
     7801 gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcc aaatggcgac cagaccagga
     7861 cgctctggag ttatgtcgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc
     7921 gctgccacag agcacggtga atacggtggt taaagaagaa acctctgccg ccacgacggc
     7981 tgacctcggc ccacggatgc aatgcagcgt ctgccagtgg atttacgatc cggcaaaagg
     8041 cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct
     8101 gtgcccggaa tgctctctcg gcaaagatgt ctttgacgaa ctggcatcgg aggcaaaatg
     8161 agcaacggca ttgtgatcat tggttcgggc ttcgccgccc gccaactggt gaaaaatatt
     8221 cgcaaacagg acgcctgtat tccattaacc ctgattgccg ccgacagcat ggatgagtac
     8281 aacaaacctg acctcagcca tgttatcagt caggggcaac gtgccgacga ccttacccgc
     8341 cagacggcag gtgaatttgc cgagcagttt aatctgcgcc tgtttccgca cacctgggta
     8401 acggatatcg atgccgaagc ccatgtggtg aaaagtcaga ataatcagtg gcaatacgac
     8461 aagttagtgc tggcaaccgg tgccagcgcc tttgtcccgc cagtgcccgg gcgtgagtta
     8521 atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacacaact gcgggatgcc
     8581 cgacgcgtgt tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt
     8641 cgggcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg
     8701 ccaccggaag tcagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg
     8761 ttaaaatctc agttgcaagg actggaaaaa acagattctg gcattctggc aacgctggac
     8821 cgccagcgct gcatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaacc
     8881 gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg
     8941 caaaccagta atgccgatat ttatgcgctg ggcgattgcg cggaaattaa cggtcaggta
     9001 ttgccgttcc tccagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc
     9061 aataacacgc cgctgaaact cccggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg
     9121 ctgcatctgg caggcgaacc ccagcgtcag gatttacgct ggcaaattaa taccgaacgc
     9181 cagggaatgg tggcgcgcgg tgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt
     9241 gaggatcgga tgaaagaggc atttggattg ttgaaaacgt tgtcgatgta ggcgagctac
     9301 agtgccccaa atgtcggatg cggcgctgac gcgtcttatc cgacctacag ggaacgcatg
     9361 tgttgatcac ataatacgtt tatgtcgcat tgcactgtcc caaaatgtcg gatgcgacgc
     9421 tagcgcgtct tatccgacct gcggggatga atatgtaggc cggataaggc gttcacgccg
     9481 catccggcaa tggtgtccaa atgcaacacg ctttatccgt tctggacttc acccgctaac
     9541 caacgcgccg cagcaataac cccctgcccc agagacaacc cgccatcgcc cgcaggtaaa
     9601 ctctgtggaa agagcaatgt gaaatcagcg agataatgcg ccagacgtgc acgcagcaaa
     9661 cggttatgaa taaccccgcc gctaaatacc agcgtagtga taccacgcat cgtggcctgc
     9721 tcacgcatca acgcggcccc cccctgcgcc agcgcatcat gaaacgccca cgcacgttga
     9781 ttaaccggag cctgccagtt cagccactgc tgccagaagg tggcgagatc cagttgatta
     9841 tccaccagcg gcatcgtcac cggatgcgtc acgccgtggc acgaagtcgc cagcgcctcc
     9901 agagcacaag ccgcttcacc ttcataactt aacgtggctg gcgcacagcc cagcgccgcc
     9961 gctacggcat cgaacaaccg cccacacgat gacgccagtg gtgcattgat tccacgctca
    10021 atggcccgcg ccagcacgct ccagttttgc tgttgcacac ttaccgtttc gggataactc
    10081 tgccactccg gcacaaaacg caagcactgc gtcagcaggt ttcgccacgg ctgctttgcg
    10141 gcaagatcgc caccagcaag cgccactgca ggcaagccgc ccaggtgctc gcattcacga
    10201 tagttcaccc gcaggcactc tcccccccac aaagcgccgt tctcccccat accaataccg
    10261 tcgagtgtca acgcaatgac atctccgcca tccagcggcc agtgatgttc tgccagacat
    10321 gccgctgcgt gagcatgatg atgcagcacc gtttgcgtcg gcagattcat ttcgcgcgcc
    10381 cactggctgg agacatagcc cggatgcgcg tcatgcacaa cgtattgcgg agtgaaatcg
    10441 tagatgtttt gcatcaggcg taacgcttcg cgccactgca tctggatgcc atcgtcactt
    10501 aaatcgccca gatgctgact caacaccgct tgttcgccgc gcaccaggca gaaggtattt
    10561 ttcagatccg cgccgagaca cagcacaggc ggaacatttt taaagcccgg aggcaaagcc
    10621 agcgcatccg gcacataccc ccgcgaacgg cgcagcattt cgccgctttc gcgcaccacc
    10681 gaatcatcca tccgctgcac gatgtcgcgg ttatgtatca agaatccgtc ggcaatgccc
    10741 tgcaaatccg ccagcgcctg ttcgttgctg atagccggtg gtttaccgct caggttgccg
    10801 gaggtcatca ccagcgggca ttgcagttcc tgtaacagca aatgctggag cgggttcgca
    10861 ggcaacatta ccccgacttc gttaaggcca ggggcgatat catcacaaag ctcaggtaca
    10921 tattttttat ccaccagcac aatcggcgcg gcgggcgtgg taagcaactg gcgcgcagcg
    10981 tctggtaaac cgtcagccac tggcaacatg accgccagcg gtttcgccgg gcgatgtttg
    11041 cgcgcccgaa gtgtcgccac cgcgttactg ttacgtgcat cgcaggcaag atgaaatccg
    11101 ccaatccctt tgatggcgac aatgttgccc atttttaact gtgcgatagc tgcctgtaat
    11161 gccgcctctt gttctgcatg ttcaccatga cttacccatt caagatgggg gccacactcc
    11221 gggcaggcca ccggctgggc gtggaagcga cgatcgagcg ggtcacagta ctctttgtca
    11281 caagccggac acagcggaaa cgccgccatc acggtaaacg ggcggtcgta aggcatggcg
    11341 cgaataatgg tgaaacgcgg gccacagtgg gtacagttga taaacggata acgataacgc
    11401 cgctcgcccg gggtattcat ttcggcaagg caagcagggc aagtcgcggc atcgggaact
    11461 atttgcgtat tcatagcgcc gcctgtgctc tggcggatag tgaactcggt gggcagttgt
    11521 gaccagataa acggctcacg ctcgacgcta tcaatacgcg ccagcggcgg acaatgctga
    11581 tgcaattgaa caagaaaggt ttccggctct tccagcagcc ggacttctac gccatcgccg
    11641 tcattacaga catcgccgtg aagatttaat tgctgtgcca gttgccagac aaacggacga
    11701 aaaccgacgc cctgcacttt gccacgaata cgcagttgga caccgcaaga tgtgtttttt
    11761 gccattgagt tattcccgcc atcatgaatt gcgtaacccg cccagccgga cacgacagcg
    11821 tcgcatccgg cagtcacagg tcggcgatac tgcccgcttc gtattctacg aatatttccg
    11881 ggaattcctt tgataccaga acagttctgt aagattttta gaacatcagc gccgtgcggc
    11941 ggcgtttttc tgcgctcagt tgttcaagtt tattacgatc gacacaaatc agcgcatggg
    12001 tcgggcaagc cgccatacac gccgggccgt cttcacgatg gttgcacagg tcgcatttat
    12061 tggcttcggc tttgtcagcc cgtacattca gccctgcgcc gctgttgcga atcaccggac
    12121 gcaccaccac ttccatcgca ccatatgggc aagccacaac gcaggttttg caaccaatac
    12181 aacgttcctg catgacatga acaaaccctt tatcacggct gatagcacca ttcgggcaga
    12241 cgttagcgca cggtgcatct tcacactgac ggcagactgt cgccgtggaa atgttcacac
    12301 ctttaatgac atggattcgc ggtaaaaatg tttccggggt cagcgatgca cagtcctgat
    12361 tttcctgatg agaaaccacg cacgccactt cacaggtacg gcaaccaata catttactcg
    12421 cgtcagcaat gatgaaacgg ttcatcaaat tctccagcaa taacagttaa tgcgccgata
    12481 cattcacaaa tcatgccagt ttttaattta ctgttattta aggaaattaa attctgtact
    12541 acaggaaaaa cgatgtcatc gacactagtg tcgatgacat gtgatgacag tatttatcgt
    12601 agtaaggcag agagaatccg ttttctaatg aatattttat cgttgccagt ccacatctgg
    12661 gtactttttt ccgacaagtc gttctggtgg tataaaaaag taattgccag taacggggag
    12721 gatttaaggg aagcaaccgt gccggatacg gcgtgaacaa cgaccacttt gtcagctatc
    12781 catatgattg tattttgagg ttcatcgcag attagcgatg aacctttatt gaataatact
    12841 atttcgactg aaataacagg taaggataca aaatgggttc atggggaatg gaggctctcg
    12901 aaagcgacga agggctggat ttgattaatt gggtagagga acaactgcaa gatgattcga
    12961 cctttgatgc cgagtcaatt gttcaacgct taagccagca tgaagatttg ttcggatttc
    13021 agggagatga agaatttctc tacgataatt acgataataa tgttatcggt ctcgttgaac
    13081 ttatcattca aaaagcagcc gggaaaaaaa tcacatcgag taaacaaatt gatcaactcg
    13141 acggttatca gctcacgtcg actttcagca aaaaactcca gggccgttta caaacaatcg
    13201 acgacacaca tgagtggata atgcttttcg aggggcgagc acgcgaaaaa gcaaaagcat
    13261 atctgattga gcttactgat aaattaagag tagtaaagac aacagcctga atgacaaaca
    13321 agggcgcgca aaattaagaa tctgcgccct ttcaaagccc gataggcgta gcgcatcagg
    13381 cgatttagcg tctgccatca gtcaaagaaa ccattccggc ttaacgccaa atcgggctga
    13441 aagtgcctta atgtgggata tcgtcaggga acgttgtcca gataaaatct ggctgaccag
    13501 ggatttagag ccgatttcgt tcttcaaatc tgcataggtc agattatgtt gatcaatcag
    13561 cgtgcgaagt aacgccacac caacaggcat tgctgcaacg gctttgtcga actctgcaaa
    13621 cttttcgtta ttgttttcat attctgcaat gcggctcgcc agaaagtcga taagtggatt
    13681 agtatcatct tcttcaatca aatattcgac cagttccagt gcttcacggt aatcatcttc
    13741 tgagtcactg cctcccagaa aggggactgc attgaccagt tctcttgttg ctttaacagc
    13801 gcgtgcagca ttagcagtca ttctttgttc tctctgtagt aacgtgtcag acgcccactt
    13861 ttctaaaatg ccaggtttgc tcggcatcat aaggcatggt caggagaggt ttcagcgccc
    13921 gcttttgcgc gtattcgctg tcactcattg gcagatattc ggtaaagctg gtggcctgtg
    13981 caggcagcac taagctcaat aaaaaagcgc acatccctgt tttgatgttc aattccctga
    14041 attccctaat gaagaattat tcttccaccg ctaactgcgc gaagccgcca tttccttccc
    14101 agccttccag ccgctgatac accgtttcca ccgcatcttt aatcggctga gtcatcgggt
    14161 agtaaaagcc gacgatatcc ggctggatgc caaggaaaat cacttcgcca atatcttctt
    14221 tcaactggtc gataagatag ttcagcggca tgttatgggt agtcatcata aacatctcgg
    14281 cgatgtcagc cgggtcgatg atgcggatct cgccagggtt tagccccata tccgtggcgt
    14341 cgacaatcag cagccgtgtc gggcgcagtt cgcggatagc gacaatgtcg ttttccggtg
    14401 cgctaccgcc gtcaatcact acccagttac ctttcggcgc ggcggcgcac ttttccgcca
    14461 gtagcgggcc tgcgccatca tcgcccatca tgctattgcc aacacaaagt aaaacgtcag
    14521 tcacgcagtc tcctcaccat caaatagatg gcgttttcct gatgaatatc atgcagcata
    14581 cttaacagca ttgtactcca ttccttctgc tgcgaagttt gcgctgcccg ggctttatcg
    14641 aatgcgttgg ccagcatcgg cacatggtta atgtcgatga cgatctcacc atatttcggc
    14701 acgccttcca ttttacggcg ggcttcgctg cccgcctcca gagtggcaat ccatgccaga
    14761 tagtcatccc acgggcaggt aagtgccgct tccaggcaat cgataacccc aaggtggtga
    14821 ccaatcgcca ggctgtaata gaccacctgc tgcgcctcgg cgggcgtggc atcgttctca
    14881 tcaataaatt tacggctcag ttgactgaac accacctttt cactcatcgg atacgcgcct
    14941 cttcaacaac atgattcaga tggctgacaa tctcgttcag acgcggatcg ttttccgctt
    15001 ccagccagcg tgcaacctgt tcttcgccct gccctaactg tgtcaggaaa tcatcggcaa
    15061 tctggcgacc gtaacgataa cccgccagac gacgcgcttc acggtccact ttcacacgca
    15121 acggttgcac catatcagga tgtagaatgt ctgccggctg ctcatcttgc tcacccggcc
    15181 cacgggcgtg gattttctgc tccagcaggc cgagcgccat cgcaaagccg tacagcgtgg
    15241 cggcaggcgt tggcgggcag ccagggatgt agacatctac cggaacgatt ttatccgtac
    15301 cgccccacac gcagtagaga tcgtggaaga tcccgccgct gttaccgcag gcaccgtaag
    15361 agatacagat tttcgggtcc ggcgcggact gccacgcacg cagcgcaggg gatcgcattg
    15421 cacgggtgac cgcgccggta aacagtaaaa tatccgcatg acgcggtgac ggaacgactt
    15481 taatgccgaa gcgttctgca tcgaacagcg gcgaaagcgt ggcgaaaatt tcgatttcgc
    15541 aaccgttaca gccgccgcag tccacgcgat aaacgtaggc agaacgtttg atttttttca
    15601 gtaacgacgc cttcatgctg gcgatggatt catccaccgt catcgggacc ggaatgccgt
    15661 tggcgtcacg ggggcctaat aaattgctca tcagatggcc tctttcatat ggcgagtcag
    15721 ttcaatacgg tcggacggca ccaggcattt ctggcgctta cattccgggc aagtctcaaa
    15781 gctttcgcgg tggttttccg cgcggctgtc gccgttgtgc ttaagcagcg caatggcgta
    15841 gtcgatctct ttctggacgg cgaaaggacg attgcagacg cggcagttgc acagcgcgaa
    15901 gcgggactgt tgcaggaagt cttctttctt ccacaccgcc agttcgtatt cttgcgacag
    15961 cttaatcgcc gccgtcgggc agacttcttc gcagcgtcca cagaagatgc agcgtccaag
    16021 attgaactgc cacgccagct ctcctgtggc gaggtcagtt tcaaccgtta aggcgtttga
    16081 cgggcaggca ttgacgcagg ccgcacagcc gatgcactgc tgcgggttct gctctggctt
    16141 accacggaag tttttatcaa ccgcaatcgg ctccagcgga taagacgaag tcgccgtgcc
    16201 ggttttgatg acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt
    16261 tttacgctca atgctgtagc gctcgagttc tttgtacggc accactttgc tcttcttctt
    16321 acgtacatcg accacggtca tgcggtcggt acaggagtag caagggtcca ggctaccgat
    16381 aatcagcggc gcatcggaaa cggtattgcc acgcagcatg tagcgcaggg tcggccagtt
    16441 cgcgtaggtc gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct
    16501 ccagtggata tcgtcgccgc gcggcgcttc ggcaaagccc agcgcgaaac ggtgcggaat
    16561 gtaggtaaag ccttccacca tcagcgggcc gcccggcagg ttatccagac cgtaatcgat
    16621 catattcagc gcggtataga cttcgttgat acgcactttc agacgggaaa taacgtcgca
    16681 gccctgctcg ctgtggactt ccattggcag caggccataa ccaacaaacg ggtgatcggc
    16741 gcgggtatcg cgagcgtgtc cgctggcgcg gaccatcggg ccaacgttac tgaaatcgcg
    16801 ggcaatttcc gggtccagac gaccaatgcc gacggtgcgc tgttccatgt tcggtgtgct
    16861 cagcagcaca tccaccagct cctgcacttc acgacgcatc tgttgcgcca gctggcgggt
    16921 ctgaatcatg tcgtctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta
    16981 ggttttacgc gcaccggtaa ggatctctgc cattttcatg gaggtttcac gcacgcggaa
    17041 gaactgcata aagccggagt caaagccggt aaagtgacag gccaggccga ggttgagcag
    17101 atgcgagtgc aggcgttcta cctccagcag aatggcgcgg atcatctgcg cgcgttctgg
    17161 cacctgaata cccatcgcgt tttccaccga cgtggtatag gcggtgctgt gagcaaagcc
    17221 gcagatcccg cacacacggt cagagaggaa ggtcacttcg ttgtaaccca tacgggtttc
    17281 cgccagtttt tccatgccgc gatggacgta gaacaggcgg tagtcggcgt cgataatgtt
    17341 ttcaccatcg acgaacagac ggaagtgacc cggttcgtca gaagtgacgt gcagtggacc
    17401 aatcggcacg acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc
    17461 atcggtagtc ggtgccggac gctgacgata atccatgctg tctttacgca gaggataaag
    17521 ttcatccggc cagtcatccg gcagcaccag acggcgttca tccggcagac caaccggaat
    17581 caaaccgtac atatcgcgca cttcacgctc gccccacacc gccgccggaa cgcgcggcgt
    17641 cacggaagga tactccggtt tgttggcgtc aacttcaacg cgaaccgtta cccaacactt
    17701 ggtgcccttc tccatcgaca gcacgtaata aacggcgtaa tgaccattca gtttgcgttc
    17761 gtcgttacca aacagcaccg acagccagcc accctgcttg tagtagagaa actccaccac
    17821 ttccggcagg tagttcacct ttacggtcac ggtcagctga tctttggtct gccaggcgtg
    17881 gtccagcacg acgccaggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt
    17941 ttcttcagac atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa
    18001 aatgcaaagc caaacccggc ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc
    18061 atgctgtttt cgaacagcgc gataaccagg acgccaacca ccagtttgac gatggcaatc
    18121 accagcgcca gcagcagccc accgacggtg aaggtttcca tttgtcccca cggaataaac
    18181 accccgacga acatctgcaa caccaccagc tgtttcaggc tgatacccca tttcatgacg
    18241 ccaaagccgc tgccgctata ctcagagagc ggaccttcct gcaactcctg ctcggcttcc
    18301 gccaggtcga acggcagttt gcccatttcg ataaaggtgg cgaatgcaca ggcacaaagc
    18361 gccagcacca gcgggatgct ctggctcagt ggccagtgat aaacggtgtc ggtgatgttg
    18421 ctgatgttgg tggaaccggc aacctgtgcg gcgacccaca gaccaagcag cagcatcggt
    18481 tcaaccaaca cgccaagcat cgcttcacgg ctcgcgccga tagcggtaaa cgggctaccg
    18541 gtatccagac cagaaatggc aaagaagaaa cgcgcgatgg cgaagagata cagtaaggtg
    18601 atcaaatcgc ccagttgcgg cagcggagaa ccgacggtca ccaccggcag cgcagtggcg
    18661 atagtcagca tgacgcccac catcacatac ggcgtcaggc ggaacaccct gccggaggca
    18721 tccgggccaa cgctctggcg gcccagcagt ttgataatgt cgcgatactc ctgcaacaca
    18781 cccggcccac gacggttatg cagacgggcg cgtgctacgc gggtaatacc ggagagcagc
    18841 ggcgcaacgg caaatagcac cagcgcctga attaacggat ataaaacact cattctcagg
    18901 ctcctcgtga aacaataatc accaccagca ccgccagttc aaccagcgcc atccggcgga
    18961 acagcaacgc actcccctcg cactgccagc ccggcaccag agacaccgga ttcagccatt
    19021 tgcgtagttt cagcaccggc gcaaacgcct gtttcaccgg catggcaaaa ccgtgagcgg
    19081 taatcaccat tgatttttca tgatcgtagc cgcacaccca ggccgcgccg cgggaacgcg
    19141 acggcaaacg atcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa
    19201 tcaacaacaa cgtgatcatt ggttgagaaa cggtggtgtt agcaggctcc agcggcagag
    19261 gtacagcagc agagagcatc ggcagtagcc acggcgcagc aacaccgcca attacgcagc
    19321 aaatcgccag tgccactacg cttacgctca tcaggagcgg cgaacaggtg gcgttttcgg
    19381 cttctttggt acgcggcgcg ccgaggaaag taacgccata aactttcgcc atacacatca
    19441 ccgccagcgc accggtaatt gccagcccca cggcgagcag cggcccgaga agacgggcaa
    19501 caaacgcgcc actattgctc agtttgaaga aggattgata gataacccat tccccggcaa
    19561 aaccgttcag cggcggcagc gcagccattg ccatcagccc gactaacatg gcgatggaga
    19621 taaccggcat tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga
    19681 accagacgct ccccgcacca aggaacagta cgctcttgaa caggctatgg ttaagcagat
    19741 ggtacagacc accaaccaga ccaagggcaa tcagcgccgg ttgttcgagc gcgatacccg
    19801 ttacgccagc gcccagcccc agcaggatga tgccgatatt ttccagggtg tggtaagcca
    19861 gcagacgttg gatattatgt tccatcaacg catacagacc gccgacgaat gcggtgatca
    19921 tgccgagtac cagcagtgcg atcccccacc acagcggtgc attaccgccc agtagtgaca
    19981 gagttaaaat gcccagcagg ccaattttca tgactaccgt ggaaaacagc gcggcagctg
    20041 gcgcagaggc gttggcatgt gcctgtggca cccagccgtg cagcggaata atcccggcca
    20101 gcaggccaaa gccaatcaca ccgagcagcc agatatcgga accgagcggc agctgttgca
    20161 tacgcatatc cagcaggcgc agatccagcg tgccgtaacg ctgccacagc agccagcagg
    20221 caatcgccag cagcagagtg ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc
    20281 tgttgctggt gaggaacacc gcacacaggg ccatgatttc ggccattacc acgaacatgc
    20341 cgaggttgct ggcgatgacg gcacagacgg cggcagccat caacatattg atctgcaagc
    20401 cgttgcactt cacctgcgcg tggcgatgcc agtcaatgtt gtagaggctg acaaacagac
    20461 cgcacagacc gagcgtaatc agccaaatcg cgttaagcgg agagatttgc acatcgtagc
    20521 ttaccaacga cagcgcaccg ctcacgccaa ccgcgccagt cagcacagtg aagcccgcgg
    20581 ctgccgtata caggctaccc accgcgccgc caattccggc tatccagcca cttagcgctt
    20641 tttgaaaaga aaagagaaat gccagaacag cggcggcgac aaaccacgcc acgccgctat
    20701 tgatcaggga aattgcgctc atttagcctc tccactttga gcctgctgaa acaaggtgag
    20761 atcgccaaag tccgtgttaa aagtcagctc acgcttacgt ttgctgacgc gggcgatatc
    20821 ggtgttatcc accagatgca gggctttagt cgggcacatc cgcacgcagg ccggaccttg
    20881 ttcatcaaag ctacaaaggt cacatttgac ggcgatcgcg cgaatacctg gcacccagtc
    20941 aagcaatgtg ctgacacgcg ccggagcagg cggtgccggt ggcgctttcg gggtattggt
    21001 gtttgccgga atatccagcg gacggctgcc ggaaaattca attgcgccaa acgggcaggc
    21061 gatgccacac agtttgcagc ttacgcacaa gctttcattc agctgtacgg caccatcaac
    21121 acgagtgatg gcgttaaccg ggcagaccgt tgcgcagggt gcatcttcac agtggtgaca
    21181 gagctgcggc gcagattctt tttcattgag catcactcgc aggcgcggca ttgattgcag
    21241 gccgtgctgg cgatgcgtct ctgaacaggc agcctcacaa gtgtggcagc cgatacagag
    21301 cgtggagtca gcaattacaa aacgattcac caggcattcc tcaggtgatt gtcatttttg
    21361 acgaaaacat gccgatgaaa tgtcattttc gacactcatc gacacgccca tccccaaaca
    21421 ggcgtaacgc ctgcaaaacg ggcaaagcct cagctcatgc taccgggctt tgtcccttta
    21481 ccagttggct taaattcacc ggcacattgt tttcaatggc ggtatataag ctgtcgtaaa
    21541 cgccagcgat tttttcgaga tagtgttcca gaacttgttc gcgatcgcgg atgttgacct
    21601 tgccatcaat catgccgtca atgctcaact gcgcctgcgc aatccgctgt ttgtcttcgc
    21661 catcttttgt ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc gccagggtga
    21721 cgtaaatgcg aaaatgttca aaaaggacga aacagagttc tttgtccggc gcgcagctca
    21781 tggcgtgatg caggcgcgct ttcgataacg tgatcccctg aaccagcgaa ttgcagtaga
    21841 tgtgccactg gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta
    21901 tttcccaaat agtcattgtc aggttacccg tttaacagag atgccagctt taagcatttt
    21961 ctgtgccaac ttttaattca ttgttattaa agcgatttct aagctaaaga tgaatttcgt
    22021 cgccgtgtcg acgtgtcatt tcgacatcat cgacattatt caccgcaggg ataatcaaca
    22081 ctggcacaat tattgcttgt agctggcaat agttaatggg aggcgatatg cacgaaataa
    22141 ccctctgcca acgggcactg gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac
    22201 gcgtaactgg ggtctggctc aaaattggcg cattttcttg tgtcgaaacc agctctcttg
    22261 ccttttgttt tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg
    22321 aagaacaaga ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc
    22381 agcgcgtccg ccgctgtcca cagtgtcatg gtgacatgct acagattgtg gcagacgacg
    22441 gtttacagat tcggcggata gaaatagacc aggagtgagc gatgtgtaca acatgcggtt
    22501 gcggtgaagg caacctctat atcgagggtg atgaacataa ccctcattcc gcgtttcgta
    22561 gcgcgccatt tgccccggcg gcacgcccga agatgaaaat caccggcatt aaagcgcctg
    22621 aatttacccc cagccagact gaagaaggcg atctgcatta cggtcatggc gaagcgggca
    22681 ctcacgcgcc gggcatgagc cagcgtcgga tgctggaagt cgaaattgac gtgctggaca
    22741 aaaataaccg tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag caactggtgc
    22801 tcaacctggt ttccagccct ggttccggta aaaccaccct gctgacggaa actttaatgc
    22861 gcctgaaaga cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg
    22921 ccgcacgcat tcgcgctacc ggcaccccag cgattcaggt gaacaccggt aaaggctgcc
    22981 atcttgacgc acagatgatt gccgacgccg cgccgcgcct gccactggac gataacggta
    23041 ttctgtttat cgaaaacgtc ggtaacctcg tttgcccggc cagcttcgat ctcggtgaaa
    23101 aacacaaagt ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg aaatatccgc
    23161 acatgtttgc cgccgcctcg ctgatgctgc tcaacaaagt tgacttgctg ccgtatctca
    23221 actttgacgt tgagaagtgc atcgcctgcg cccgtgaagt caatccagaa attgaaatca
    23281 tccttatttc cgccaccagc ggcgaaggga tggaccagtg gctgaactgg ctggagacac
    23341 agcgatgtgc ataggcgttc ccggccagat ccgcaccatt gacggcaacc aggcgaaagt
    23401 cgacgtctgc ggcattcaac gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa
    23461 cggtcagcca cgcgtgggcc agtgggtact ggttcacgtt ggctttgcca tgagcgtaat
    23521 taatgaagcc gaagcacgcg acactctcga cgccttacaa aacatgtttg acgttgagcc
    23581 agatgtcggc gcgctgttgt atggcgagga aaaataatgc gttttgttga tgaatatcgc
    23641 gcgccggaac aggtaatgca gttaattgag catctgcgcg aacgtgcttc acatctctct
    23701 tacaccgccg aacgccctct gcggattatg gaagtgtgtg gcggtcatac ccacgccatt
    23761 tttaaattcg gcctcgacca gttgctaccg gaaaacgttg agtttatcca cggtccgggt
    23821 tgcccggtgt gcgtactgcc aatgggtaga atcgacacct gcgtggagat tgccagccat
    23881 ccggaagtca tcttctgtac ctttggcgat gccatgcgcg taccagggaa acaggggtcg
    23941 ctgttgcagg caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc aatggatgcg
    24001 ttgaaactgg cgcaggagaa tccaacccgt aaagtggtat tcttcggctt aggttttgaa
    24061 accaccatgc cgaccaccgc catcactctg caacaggcga aagcccgcga tgtgcagaat
    24121 ttttacttct tctgccagca cattacgctt atcccgacac tgcgcagttt gctggaacag
    24181 ccagataacg gtatcgacgc gttcctcgcg ccgggccacg tcagtatggt tatcggcact
    24241 gatgcctata attttatcgc cagcgatttt catcgtccgc tagtggtcgc tggtttcgaa
    24301 ccccttgatc tacttcaagg cgtggtgatg ctggtggagc agaaaatagc ggcccacagc
    24361 aaggtagaga atcagtatcg tcgggtggta ccggatgccg gtaacctgct ggcgcaacag
    24421 gccattgctg atgtgttctg tgtcaacggc gacagtgaat ggcgcggctt aggcgtgatt
    24481 gaatcttctg gcgtgcacct gacgccggat tatcaacgat tcgatgccga agcacatttc
    24541 cgcccggcac cgcagcaggt ctgcgatgac ccgcgtgcgc gttgtggcga agtcttgacg
    24601 ggcaaatgta agccgcatca atgcccgctg tttggtaaca cctgtaatcc tcaaaccgcg
    24661 tttggtgcgc tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag
    24721 caggagagtg aagcgtgaat aatatccaac tcgcccacgg cagcggcggc caggcgatgc
    24781 agcaattaat caacagcctg tttatggaag cctttgccaa cccgtggctg gcagagcagg
    24841 aagatcaggc ccgtcttgat ttggcacagc tggtagcgga aggcgaccgt ctggcgttct
    24901 ccaccgacag ttacgtcatt gacccgctgt tctttcctgg cggtaatatc ggcaagctgg
    24961 cgatttgcgg caccgccaat gacgttgcgg tcagtggcgc aattccgcgc tatctctcct
    25021 gtggctttat cctcgaagaa ggattgccga tggagacact gaaagccgta gtgaccagca
    25081 tggcagaaac cgcccgtgcg gcaggcattg ccatcgttac tggcgacacc aaagtggtac
    25141 agcgcggcgc ggcagataaa ctgtttatca acaccgctgg catgggcgca attccggcga
    25201 atattcactg gggcgcacag acgctaaccg caggcgatgt attgctggtt agcggtacac
    25261 tcggcgacca tggggcgact atccttaacc tgcgtgagca actagggctg gatggagaac
    25321 tggtcagcga ctgcgcggtg ctggcgccgc ttattcagac gctgcgtgac attcccggcg
    25381 tgaaagcgct gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat gagttcgcgg
    25441 cagcctgcgg ttgtggtatt gaactttcag aagcggcact gccggttaaa cctgctgtgc
    25501 gcggcgtttg cgaactgctg ggactggacg cccttaactt tgccaacgaa ggcaaactgg
    25561 tgatcgccgt tgaacgcaac gcagcagagc aagtgctggc ggcgttacat tcccatccat
    25621 tgggaaaaga tgcggcgctg attggcgaag tagtagaacg taaaggtgtt cgtcttgccg
    25681 gtctgtatgg cgtgaaacga accctcgatc taccgcacgc cgaaccgctt ccgcgtatat
    25741 gctaataaaa ttctaaatct cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg
    25801 ctttcctgga agaacaaaat gtcatataca ccgatgagtg atctcggaca gcaagggttg
    25861 ttcgacatca ctcggacact attgcagcag cccgatctgg cctcgctgtg tgaggctctt
    25921 tcgcaactgg taaagcgttc tgcgctcgcc gacaacgcgg cgattgtgtt gtggcaagcg
    25981 cagactcaac gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat taaatatgaa
    26041 gacgaaactg ttctggcaca cggtccggta cgcagcattt tgtcgcgccc tgatacgctg
    26101 cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc tggccacagg tgggctatac
    26161 cccaaatttg gtcactattg cctgatgcca ctggcggcgg aagggcatat ttttggtggc
    26221 tgtgaattta ttcgttatga cgatcgcccc tggagcgaaa aagagttcaa tcgtctgcaa
    26281 acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc agagccgcgt cgttaacaat
    26341 gtcgactatg agttgttatg ccgggaacgc gataacttcc gcatcctggt cgccatcact
    26401 aacgcggtgc tttcccgcct ggatatggac gaactggtca gcgaagtcgc caaagaaatc
    26461 cactactact tcgacattga cgatatcagc atcgtcttac gcagccaccg taagaacaaa
    26521 ctcaacatct actccactca ctatcttgat aaacagcatc ccgctcacga acagagcgaa
    26581 gtcgatgaag ccggaaccct caccgaacgc gtcttcaaaa gtaaagagat gctgttgatt
    26641 aatctccacg agcgggacga tttagccccc tatgaacgca tgttgttcaa cacctggggc
    26701 aatcagattc aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat gctgggcgta
    26761 ctgaaactgg cgcaatgcga agagaaagtc tttaccacta ccaatctgaa tttactgcgc
    26821 cagattgccg aacgtgtggc aatcgctgtc gataacgccc tcgcctatca ggaaattcat
    26881 cgtctgaaag aacggctggt tgatgaaaac ctcgccctga ccgagcagct caacaatgtt
    26941 gatagtgaat ttggcgagat tattggccgc agcgaagcca tgtacagcgt acttaaacaa
    27001 gttgaaatgg tggcgcaaag tgacagtacc gttctgatcc tcggtgaaac tggtacaggt
    27061 aaagagctga ttgcccgcgc gatccataat ctcagtgggc gtaataatcg ccgcatggtc
    27121 aaaatgaact gcgcggcgat gcctgccgga ttgctggaaa gcgatctatt tggtcatgag
    27181 cgcggggctt ttaccggtgc cagcgctcag cgcattggtc gttttgaact ggcggataaa
    27241 agctcactat tcctcgatga agtgggcgat atgccgctgg agttacagcc gaagttgctg
    27301 cgtgtattac aggaacagga gtttgaacgt ctcggcagca acaaaatcat tcagacggac
    27361 gtacgtttaa ttgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga ccgagagttc
    27421 cgtagcgatc tctattaccg cctgaacgta ttcccgatcc acctgccgcc actacgcgag
    27481 cgtccggaag atattccgct gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg
    27541 gggcgcaata tcgacagcat tcctgccgag acgctgcgca tcctgagcaa catggaatgg
    27601 ccgggcaacg tgcgtgagct ggaaaacgtc atcgagcgcg cggtattgct aacacgcggc
    27661 aacgtgctgc agctgtcatt gccagatatt gctttaccag aacctgaaac gccgcctgcc
    27721 gcaacggttg tcgcccagga gggcgaagat gaatatcagt tgattgttcg cgtgctgaaa
    27781 gaaacaaacg gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc
    27841 acgactctgc tgtcacgaat gaagcgactg ggaattgata aatcggcatt gatttaacag
    27901 atgtggttat actcgatcaa cattcaatga aaggaggcag tatggcagtt tcagcaagaa
    27961 atcaattaac cggaacagtt agcgcagtag caatgggcgc ggtgaatgac gaagtagaac
    28021 tgacgctggc aggtggtgca aagctggtgg caattgtgac tcacagcagc cagcaagcgc
    28081 tgggtctggc gaaagggaaa gaagcgattg ccctgattaa agccccgtgg gtaacgctgg
    28141 caacggaaga ctgtggactg aaattctctg ctcgcaacca gtttgctggc agcgtgtcga
    28201 caatcaccga aggcgcagtg aacgccactg tccatatcaa aacggatgcg ggttttgaaa
    28261 ttgtggcagt ggtaacaaat gaaagtcagg atgaaatgaa actcaccact ggctcgcgcg
    28321 ttattgcatt gatcaaagca tcagccatcc tgattgccac caaagcgtaa gcgccgtatt
    28381 tgccggagag tatggctctc cggcagattt atttctttgg acgatatttt tctggcaact
    28441 ccggcaccgg acgcttgtca tcgatgagat gacgcacggt taagatcgga tgacgccaca
    28501 gcattctcgg cccggcccaa cgcataatct gtttcatctc ttcacgcttt gcaggctggt
    28561 aacagtgcac cggacactgc ttacaggctg gtttctcttc gccgaacaca catttatcca
    28621 gccgcttttg cgcgtaagca aacaacgcct cgtaatgctc cggctctgct gacgcctgcg
    28681 ggcatttcgc ttgataaaga tcgatcattt ttttaatcgt cagtttttca cgagagatac
    28741 gcttgccgga catgctgcct ccatactctt aaggtgcatt tatattacaa cttaatttta
    28801 aaggggacta tggctctgaa ggaggaggtt tcttcaaccg acgaatttgg cgatcttgtt
    28861 tcatcgtcat cacgcaaaag ctgcaaagca gcatttttcg cggaaccgac atcaagaact
    28921 cacctttcgc gtcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    28981 acaacgaaaa taaaaaccat cacactacat ttaacatcag ggagccggac ttaaccccat
    29041 gagtacaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaagctgaa
    29101 agcccagcat cccgagatcc tgctgtttta ccggatgggc gacttttatg aactgtttta
    29161 tgacgacgca aaacgggcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    29221 agcgggagag ccaatcccga tggcggggat tccctaccat gcggtggaaa actacctcgc
    29281 caaactggtg aatcagggcg aatccgtcgc tatttgtgaa cagattggcg atccggcgac
    29341 cagcaaaggg ccggtcgagc gcaaagttgt ccgtatcgtt acgccgggca ccatcagcga
    29401 tgaagcgctg ttgcaggaac gtcaggacaa cctgctggcg gctatctggc aggacagtaa
    29461 aggtttcggc tacgcaacgc tggatatcag ttccggtcgt tttcgcctga gcgaaccggc
    29521 ggaccgcgaa acgatggcgg cagaactgca acgtacgaat ccagcggaat tactgtatgc
    29581 agaagatttc gccgagatgt cgctgattga aggtcgccgc ggcctgcgtc gtcgcccgct
    29641 gtgggagttt gaaatcgaca ccgctcgcca gcaattgaac ctgcaatttg gcacccgaga
    29701 tctggtcggt tttggtgtgg agaacgcacc acgcggactt tgtgctgccg gttgtctgtt
    29761 gcagtatgcg aaagataccc aacgcacgac cctgccgcat atccgttcta tcactatgga
    29821 acgtcagcag gacagcatca ttatggatgc cgcgacgcgt cgtaacctgg aaattactca
    29881 gaacctggcc ggcggtgcgg aaaatacgct ggcttcagtg ctcgactgca ctgtaacgcc
    29941 gatgggtagt cgtatgttga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    30001 gcttgagcgt cagcaaacta ttggcgcatt gcaggatttc accgccgagc tgcagccggt
    30061 actgcgtcag gtgggcgacc tggaacgtat tctggcgcgt ctggcgttgc gtaccgctcg
    30121 cccacgcgat ctggcccgta tgcgtcacgc tttccagcaa ctgccggagt tgcgtgcgca
    30181 gttagaaaat gtcgatagtg caccggtaca agcgctgcgt gagaagatgg gcgagtttgc
    30241 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccaccggtgc tggtacgcga
    30301 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    30361 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    30421 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    30481 aagccatttg gcaccaatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    30541 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    30601 ggctctggaa aaacaacttt atgaagagct gttcgacctg ctgttgccgc atctggaagc
    30661 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg
    30721 ggcctatacc ctgaactata cctgcccgac cttcattgat aaacctggca ttcgtattac
    30781 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccgtttatcg ctaacccgct
    30841 gaacttgtcg ccgcagcgtc gcatgttgat cattaccggt ccgaacatgg gcggtaaaag
    30901 tacctatatg cgccagaccg cgttgattgc gctgatggcg tatatcggca gctacgtacc
    30961 ggcgcaaaaa gtcgagattg gcccgattga ccgtatcttt acccgcgtag gtgctgcgga
    31021 tgatctggct tccgggcgtt caacctttat ggtggagatg accgaaaccg ccaatatttt
    31081 acataacgcc accgaatata gtctggtgtt gatggatgag atcgggcgtg gaacgtccac
    31141 ttacgatggt ctgtcgctgg cgtgggcgtg tgcggaaaat ctggcaaata agatcaaagc
    31201 gttgacgctg tttgctaccc actatttcga gctgacccag ttaccggaga aaatggaagg
    31261 cgtcgccaac gtgcatctcg atgcactgga gcacggcgac accattgcct ttatgcatag
    31321 cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg gttgcagctc tggcgggtgt
    31381 gccaaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    31441 gaacgccgct gctacgcaag tggatggtac acaaatgtct ttgctgtccg taccggaaga
    31501 aacttcgcct gcggtcgagg cactggaaaa cctcgacccg gattcactga ctccgcgtca
    31561 ggcgctggaa tggatttatc gcttgaagag tctggtgtaa taataattcc cgatagcctt
    31621 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    31681 aaaacttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    31741 tcactatcgc catatatggg ccgttggtga tattcatggt gactatcaat tattacaatc
    31801 ccgcttacat caactctctt tttgtcctga aaccgactta cttatttcgg taggcgataa
    31861 catcgatcgt gggccggaga gtcttaacgt gctgcgcctg ttaaaccagc cctggtttat
    31921 ctccgtcaaa ggcaaccacg aagcgatggc gcttgaggca ttcgaaactg gcgatggaaa
    31981 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    32041 ggcaatagat ctgttgctga aattccatct ccttccacat attattgaaa tcactaacga
    32101 caacataaaa tatgtcatcg cacatgcaga ttatccgggg aaggagtatc agtttggcaa
    32161 agaaatagcg gagagcgaat tactctggcc tgttgatcgt gtgcagaaat cacttaatag
    32221 cgaattacaa aaaataaacg gcgccgactt ctttatattt ggtcatatga tgtttgataa
    32281 cattcagacg ttcgctaacc agatttatat agataccgga tcgccgaaaa gcgggcggct
    32341 gtcattttat aaaataaagt agtctcatgc ttcttctgtg aagcatgagt aacccggcgt
    32401 tattgcaggt cattattcat ttttcgctac cagcaaagag agatccagct tcgccagcgc
    32461 gcggctggca ctctccggca gaccgtcgtc agtaatgatc tgatcaaact cgcttaatgg
    32521 taacgccagc catgtcgcca cctgtccata tttcgtcgca tcacagacca aaactcgctg
    32581 gcggctggca ctggcaatcg cccgtttcac cgtgacttta tcttctgctg gcgtagagat
    32641 cccccgcaca ctccatgacg atgcagaaat aaaagcctga tcaatcatca ggctacgcag
    32701 catggtcgca gcggcttccc caacacagga acggttttcc cgacacactg cgccgccagt
    32761 gtgaataatt gtgcaattac tgttgtcgag caagtagtcc gcaataacga agtcgttagt
    32821 aaccactgtg agtggctcca tgtgaatcag atgctgtgca atcgctaacg tggtcgttcc
    32881 cgcatccaga tagatacaac gtcccggctg aacaagactt gccgccagct tgccaatagc
    32941 cgctttttgc gtcattgcca gcgcagtttt tacctgatga gaaggttcat gcgccacgcg
    33001 tcccggagac tggacgcccc cggacaccag cacaacggct ccctgctgct ccagtttttg
    33061 taaatcccga cgaatggtca tatgtgacac attcattctg tccgttagtt cagcaatact
    33121 aacaatgcct ttttcagcta ccatctcaag gatgatttgg cgacgctcta cgggtatcaa
    33181 cttttgctcc ttcctttgtc ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg
    33241 gcgcaactaa cgcttagttc acataaaata acacacaatg ttaatttttg tgaatcagct
    33301 caccatatcg
//
LOCUS       DAJOTB010000037        33072 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJOTB010000037 DAJOTB010000000
VERSION     DAJOTB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.037"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            25..675
                     /gene="zinT"
                     /locus_tag="OHN28_001772"
     CDS             25..675
                     /gene="zinT"
                     /locus_tag="OHN28_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCQ2297179.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1345..1434)
                     /locus_tag="OHN28_001773"
     tRNA            complement(1345..1434)
                     /locus_tag="OHN28_001773"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Ser,seq:cga)
     gene            1528..2325
                     /gene="mtfA"
                     /locus_tag="OHN28_001774"
     CDS             1528..2325
                     /gene="mtfA"
                     /locus_tag="OHN28_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCQ2297180.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2426..2501
                     /locus_tag="OHN28_001775"
     tRNA            2426..2501
                     /locus_tag="OHN28_001775"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2459..2461,aa:Asn,seq:gtt)
     gene            2663..3925
                     /locus_tag="OHN28_001776"
     CDS             2663..3925
                     /locus_tag="OHN28_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2297181.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4119..5423)
                     /gene="ybtS"
                     /locus_tag="OHN28_001777"
     CDS             complement(4119..5423)
                     /gene="ybtS"
                     /locus_tag="OHN28_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HCQ2297182.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5451..6731)
                     /gene="ybtX"
                     /locus_tag="OHN28_001778"
     CDS             complement(5451..6731)
                     /gene="ybtX"
                     /locus_tag="OHN28_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HCQ2297183.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6724..8526)
                     /gene="ybtQ"
                     /locus_tag="OHN28_001779"
     CDS             complement(6724..8526)
                     /gene="ybtQ"
                     /locus_tag="OHN28_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HCQ2297184.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWGHE"
     gene            complement(8513..10225)
                     /gene="ybtP"
                     /locus_tag="OHN28_001780"
     CDS             complement(8513..10225)
                     /gene="ybtP"
                     /locus_tag="OHN28_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HCQ2297185.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            10482..11441
                     /gene="ybtA"
                     /locus_tag="OHN28_001781"
     CDS             10482..11441
                     /gene="ybtA"
                     /locus_tag="OHN28_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HCQ2297186.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFTPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            11632..17739
                     /gene="irp2"
                     /locus_tag="OHN28_001782"
     CDS             11632..17739
                     /gene="irp2"
                     /locus_tag="OHN28_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HCQ2297187.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQHPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            17827..27318
                     /gene="irp1"
                     /locus_tag="OHN28_001783"
     CDS             17827..27318
                     /gene="irp1"
                     /locus_tag="OHN28_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HCQ2297188.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LIFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQTLSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            27315..28415
                     /gene="ybtU"
                     /locus_tag="OHN28_001784"
     CDS             27315..28415
                     /gene="ybtU"
                     /locus_tag="OHN28_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HCQ2297189.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            28412..29215
                     /gene="ybtT"
                     /locus_tag="OHN28_001785"
     CDS             28412..29215
                     /gene="ybtT"
                     /locus_tag="OHN28_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HCQ2297190.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRNITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            29219..30796
                     /gene="ybtE"
                     /locus_tag="OHN28_001786"
     CDS             29219..30796
                     /gene="ybtE"
                     /locus_tag="OHN28_001786"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HCQ2297191.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30927..32948
                     /gene="fyuA"
                     /locus_tag="OHN28_001787"
     CDS             30927..32948
                     /gene="fyuA"
                     /locus_tag="OHN28_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HCQ2297192.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         7226 a         9833 c         9079 g         6934 t
ORIGIN      
        1 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
       61 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca cggcaaaccc
      121 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
      181 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
      241 aaacttgacc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
      301 attaaagatt attatcgcaa aggttatgcg acagatatcg aaatgattgg cattgaggac
      361 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
      421 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
      481 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
      541 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
      601 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
      661 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
      721 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
      781 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcga acaataaata
      841 cacaactcag ccatacagac tggcagcgca aatgtctcca ggagcaagtc atctgtcatg
      901 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
      961 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     1021 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     1081 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     1141 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     1201 cctgtcagac ccatagcgaa tcaagtgctg aatgtcacag tatcgaacag aaaacagtga
     1261 cgatctaacc cttcaagaat atcctacgat tgttctgttt aggaaaagca aggcgggaag
     1321 tcgggagata agtcattgat aaagtggcgg agagaggggg atttgaaccc ccggtagagt
     1381 tgcccctact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     1441 atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     1501 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     1561 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     1621 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     1681 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     1741 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaaatctta
     1801 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     1861 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     1921 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     1981 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     2041 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     2101 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa
     2161 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     2221 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     2281 cacgctaatg atacagactc gttttcggcg acgaatgttc attaattaac aactttgcag
     2341 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     2401 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     2461 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     2521 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcagtgc
     2581 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctgggg tcaggttagt
     2641 tcgataatgg agtgaccccc atatgtccct taccagcgca aaaatccgca ccctcaagcc
     2701 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgcggg tcaagccggg
     2761 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     2821 gggtacctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     2881 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgtacgtg gctcacgaac
     2941 accggagaaa gtttttaaaa acgtggcgct ggcgtggcat aaaagtaaca ggaaatggtc
     3001 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     3061 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     3121 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     3181 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     3241 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     3301 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     3361 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     3421 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     3481 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     3541 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     3601 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     3661 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     3721 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     3781 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     3841 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     3901 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     3961 ccacctccac aagaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
     4021 attttatccc aaaagaagac aatagagtca cagaggaatc aaagagcgac tcaattgtct
     4081 ttggacagtc tggttgtgag gttagaaaac agttactcct acaccattaa atagggcgca
     4141 atgctcgcta atttctcccg ggtttctgtc agttcgcgtt ccggcgtaga ttgcgcgatg
     4201 attcccgccc ccgcctgtat ccagcagcgc tggctatcct gaaatacgga acgcagaact
     4261 agcgccgcat caaagcgcgt atcgtccagc agcaggattg cgccggaata aagctctcgc
     4321 ggcgtcttct caatttgcat aatcgcgttc agagcagcat ttttagggat acctgaggcg
     4381 gtaatcgacg gaaacagcac ggtaaacgca tcccaggcat ccttgttttc cgcaagctga
     4441 ccgctcacgc cagaccccag atgctgaacg ctgccgcgct ggcgaaccga cattaaatct
     4501 tcaaccacca cgctgcccgg ctggcaaacg gcctccagtt cagcaatagc ttctttgaca
     4561 gaaaggatat gctcaagcac ctctttactg tcgtgcagca gttctgcctc tttcgcctta
     4621 ttatgctccg ggtttcccat gcgatcgcgg gtgcccgcaa gcggttcagt gaccacttta
     4681 ttgcccgtca ctgacatcac cagttctggg ctaaagccca gcgcttcgcg tccttcctga
     4741 cggaacataa acgagcgcac aggcgtgttt gcctgccgcc cgtataacag cgtggcgggc
     4801 atatcaatcc gcgatggcag gggaatggcg cgcgagacaa tcactttgac atactcgcca
     4861 cggcggatct ccgcaacggc gcgcgcaacc tgttgtttat atgcctcacc attgagggcg
     4921 gtatccaccg ccagtggtgc gttctgcgtc gttgtaccgg cctcttttac ccactcgcac
     4981 aaacgtcggc acccgtcggc ggagtccgca taaacggtga catttccctt ttcaaaaata
     5041 agttcttcac ggggaacggt taacgtcagc agcggccact ccccggcgtt aaaggcaatc
     5101 ccccgcgcat gcgccgcaaa gttgaaccca acctgtccat atacccgacg tcctttcacc
     5161 tgaggatgcg ccataaaacg acgcgcgcag tcggcaatgg aatccaccgc ccatttttgc
     5221 tccccggcat catcaataaa tgtcgcctgc gttccgtcgg cattaatgtg cagccgtgcc
     5281 tggcaccctt tgcctaaata ccaacagggt tgacgctcat agacataaca ctcttcttct
     5341 gcgaactggc gtaaaacgcc agaaatcgtc ggtagccatt gttcctctgg taacgccaga
     5401 tgtagaaatt cactgatttt catcccttac ctctctgtgt tattcgcgga tcaatcaatt
     5461 gcgcttgtca tcagttctct tgccgaatgc agccggatat aagcaaccca cgccgccagc
     5521 aacgtgaatg ccccggcaaa caggaagcag gccgcatagc caaaatgttg agcaacaacg
     5581 ccaccgataa cgcctgccag aatggcgatt gccgcatcag tacattgaaa gagggtgaag
     5641 tcgacaccgg cctgcaaagg cgatgaaagc gacatcagcg tggcgtaaag acagaccagc
     5701 gcgcaggcca gcgaaatgga ctggacaatg accagacact ggagaatctg cagcagcaga
     5761 tgacccggcg ccatcatgag cgtcatcacc accgccagca gcgcgatccc ctggacgcca
     5821 taagccgtca acagcgctct gccgggtgag gtgtatttca tcagcaatcc gccggccagc
     5881 gttcctgcta tgcccgctgc aatattgccg ccgctgaaca gcgcgcccaa tgcggacatg
     5941 ctcaacccat gatccaacaa cagcggtgcc agaagaggca gcacaaaccg catgcctgaa
     6001 ttcagcatca ataccagcag taagcccagg cgcgcctgct tcctccttag cgcataacct
     6061 aacgccggaa catgcgggat agtcgctgtt cgcgtcggct ccgtaatgcg ccacagcggg
     6121 agtgacagcg ccataatcag caccgccatc atcagcatgg cgacaggcca gccggatgcc
     6181 gccgacagca tcatgaacac cccgccgccg cacatcattc ccagatagct gccgccgacc
     6241 tgcacgctgt ttccccagcc gtaacccgtg cgggtcagtt gatcgacaca aaagccgtcg
     6301 ctggcgatat caatggtgga ggcgaccgtg cctgccacca taaacagaac aaataacgcg
     6361 acgatttgcg tatcggggaa tcccccttct cgcccaaacc agccaatcgc tgcggcaata
     6421 gtcaggatcg ccgctagcgc gacctgacca cgcaggatta acatgcggga acggcgttcc
     6481 tggctaccgg gcggaagccg ccagcgctcg atccacggcg cccaaataaa cttcagcgcc
     6541 cagggcagca taaacagggt tgtcgcaccg gcaagcgcca gcgaaccgcc agcagcgcgc
     6601 accagcgcgg gtaaggactg catagacagc gccgatacca gactctgcgt cacatagacg
     6661 ccggcaatag caaaaatcac ccgcccggtc gtcaacgtca gcggtttcac attcgactga
     6721 acatcactca tgcccccact ctccagacgc ggaaaccccg tcgtcgcgcc acacgcgcgc
     6781 ggccatttgc gcctgccaca gcgcctgata acgtccatga tgtgagagca attgcgcatg
     6841 agtaccctgc tcaaccacct gtccctcttc catcaccaga atgtttccgg ccccggcgat
     6901 ggtggataaa cggtgagcga tgataatcac cgtccggttg taaaccaggt tatcgatcgc
     6961 tttttgcacc gccagctcgc tttcaatatc cagcgcggca gtcggttcat cgagaataac
     7021 gaccggcgcg tttttcaata acgctctggc aatggaaatc cgctggcgct cgccgcccga
     7081 tagctggccg cccatctctc ccattggcgt cagccagcct tgcggaagac gggaaataaa
     7141 ctcaaggcac tgcgccgcgc gggcggcctc ttctacctcc tgccgcgtcg cctgtgggcg
     7201 cgcgatacgg atattcgcca gcagcgtgtc atcaaacagc cagacatcct ggaaaacaac
     7261 agaaatcagg ctattgagct gttccggcgt caggcggcga atatcgacgc cgccaataga
     7321 aatctgccct tgctgcggat cggcatagcg cattaacagt ttggtgacgg tagttttgcc
     7381 tgcgccggag gcacccacca gcgcgctcat actggctgcc gggaacgtca aagaaacatg
     7441 attaagcgcg tggccgtcac cttcttcgta gcgatagctg acgttgtcaa agcggatatc
     7501 gtaacgttcc ggcatctcgc tttgctctgc gacgggaagc ggtgctatcg ccataaactg
     7561 ctcaatcctt tgcagggcgc tggcgatcag ttccacaacc gaggtgtagc tgataaacat
     7621 cgccatcggt tctgcgaagc gcataatcat cgcgacggcg gcaatcaaaa aggcgaggtt
     7681 cagagtgccc gtcactaccc agacgatccc ggataacacc accacctgta ggcccaactc
     7741 cacgacgctg gcgatcagca tcgtcgcgcc agcgccctga cggtgagtgc gggtctgtaa
     7801 gttttccagc gcattgaaat gcgccagcag cgcccggctt ttatcggcat cgctgccgca
     7861 ggtgcgcaat accatcatcc cctgagcaaa ttcaacgata tcgccgctca gacgctggtg
     7921 cgcttccccc agcgtctgca tttgtcgccg catcgccggg cggcgccagt aataaaacgg
     7981 caccagcaga gggaagatca gcaacatcac cagccccagc cgccagtcta tccacaatgt
     8041 cgccagcgac gccgtcagcg gcgtgacaat ggtgagcagc aaaatattgg ctatcgcaat
     8101 aacataattg aggttttcat ccacgctgcc cagcagcaag gcgttcattt cacccgccct
     8161 gccgcgctgg agcttctcca gcggcacgcg gcgtaactgc tcgccaagtc gcaggcgcaa
     8221 ctcatgggta gcctgcgcca gatgaccacg gtattcaaag cccaggccat accagcgtag
     8281 caccagcgtc acaattgcgg cgacgctgaa ggccatagcc cagttaagaa gttgcggcgc
     8341 gtctccccgt aacagcgcat caatgatcgg ataaagacag gcaaaggcga tcccctgcat
     8401 gaatgccgcc agcaacagcg ccagcatact gcgtcgcagc atcctggcct gactgccaac
     8461 gctggagata agctggcgcc agatgacgcg ccaggccagg ttatccgcag gattattgtc
     8521 tttcattttc catcacctct tcctgtgttc cacccggcac ccaatgctgc gcctgctgat
     8581 agtgttgcca gagccgttga tacaggccgc cctgcgccaa cagttgcgta tggttcccca
     8641 tttccctgag ctgtccgtcg gaaaacagca atatcacatc ggcctgagtc accattgaga
     8701 gacgatgcgc gaccatgatg accgtccggc cacgcatggc cgccgcgagc gccttgataa
     8761 gcgccgcttc gttttccggg tcagcaaacg ccgtcgcctc atcgagcacc aggatggggc
     8821 gatcctgcaa aagcgcccgg gcgatagtaa tgcgctgccg ctggccgccg gagagaaata
     8881 ccccacgttc cccgactcga gtgttgtaac cttctggcag agcgctaata aaatcatggg
     8941 cctgcgccac tctggccgcc gctataaccg cctccagcgg cgtatccggc gcgcccagac
     9001 gaatgttatt ggctatcgtg tcggcaaaaa ggaagttgtc ctgaaacaca aacgagagtt
     9061 gcttcatcag ggtgtccgtc tgcatatcac gcagatccac gccgccaata cggatatggc
     9121 ctttgtccgg gtcggcgtaa cgcagcagca agcgcgccac ggtgcttttt ccggcgccgc
     9181 ttggcccgac cagcgccaca atttgcccgg caggcacatg aaagctcacc tcctgcaacg
     9241 cggcgccagt acgcgcttgc ggataatgaa agctcacctg ctcaaaggta atgctcgcct
     9301 cttgcggctg ctggtcagac tgcggcagcg ataactccgg catcgcgaga acctgataaa
     9361 tacgctgaat gcttaaacgc gtttgcgcga ccaggttatt gagcatcatc attggcatta
     9421 cggcttcggc cattccgctg cccagcagta aaacggccac ccacgcgata aaatcgaaac
     9481 tgccatagtg caacaggccg tatcccgacc agatcaggac aaacagggtc gggagaggat
     9541 tcagaatcga gaaggaaaaa cgcgctgaaa aaccggcttt gcgataccag gttttgagca
     9601 catcgaccca ctcttcaagg gcgcgttgat agcgcaaaaa actggtgctg ccgctatcaa
     9661 acgttctcac cacgggcatg gcctgcacga actcaataac cgccgcgcta acctgctcac
     9721 gcgccttatg ataacgctga gccatatctt ccgaacgctg gcgggcgagc acgagaacaa
     9781 ccgatccaaa cgccagtacc cccagcgtcg cgattgccag ccgccagtcc agccagaaca
     9841 agataacgat tgtcgccagc ggcatgatga tcgcgcgggc atagagcggc gtgctgtcgg
     9901 ccacaaaaat atgtaacgac ttcacgtcat ccagcatcac tttcgccact gacccgctgc
     9961 ccatttgctg taacacgcca ggcggaagct gcaatgcttt acgggctaac ctgctgcgca
    10021 gaatgttctc cagatgaaaa gccgcgtaat gagaggtgtt gaacgcctgt aagcgcagaa
    10081 caaaagttaa gaccacgcag ccgatcacgc cgcccagcgg ccaggccgga atagcgtcag
    10141 gcgtcgcgcg aatgtcacgc aggctccacg ccagaaaaag gagcgccccc aggctggtta
    10201 atgccgccag ccccgccagc gccatagcta aaataacctt cctgcgaacc ggtgtaatca
    10261 cctgccaaag aggaaatcgg gacagcgatt cagtattgga tgattgagat gacatgggag
    10321 taactgaatt tcctgatgaa tttttacgga cgccaaaaac gacaattcag ggtggcgttc
    10381 tgagaattaa tgtgaataat aaccattatc aataactcat ctaccccatt cggttctatt
    10441 tatacccgca ttggtctaag ccacagggag ataaccaggt catgacggag tcaccgcaaa
    10501 cgcaatctga aatctctatt caccagttgg tggtcgggaa acctgcaaac gatggcaata
    10561 ttcctgctca gtgtgaactg ctgcgctgtt cgttgcagga aggtatggat attttgctct
    10621 ggaggggcca ttttgcgcgg cctgagacgt tgcagctaca cgacgatctg ggcaggatta
    10681 acttctcgtg catcctggaa ggcacatcgc gctttgcgat tcagggactc cggcgtcaca
    10741 ccgactggga gcttgcgcgc aatcgccatt acattaccca tacgccagac tgtcgcggca
    10801 gcgcctccta ttgtggccga ttcgagagca ttaccctctc cttcagccct gaaaccctcg
    10861 cgctgtgggt gccggatatt agcgcggtca ttaagaataa gatcgactcc cactgctgtt
    10921 gccagcagca ccgttgtaat gctgaaaccc acttaacggc tcaggccttg cgtcacgcgc
    10981 tgatgcgcat gcacagcggc ttcagtcatg aacaaaagcc gtcgacgcta tggttgcagg
    11041 ggcaaagtct ggtcatgctc agtctggtgc tggatgaaca tcgcgaagac gccagttgcc
    11101 tctcctgcca cttcaccccg atggaacgcc agaaactgct gcgggcgaag gatctgctgc
    11161 tggctgatct gacgcaagcg ccgggcgtcg cggagctggc cagagagtcc ggtctgagcg
    11221 ttctcaaaat taagcgaggc ttccgcgttc tgttcaacaa cagcgtctat ggcctgtttc
    11281 aggctgaacg aatgcaggaa gccagaagac gcctggccaa cggcaatacc tccgtcatga
    11341 cggtggcggc cgatctgggc tatgccaacg ccagccattt ctctgccgcc ttccagaaac
    11401 agtttggcgt aactccttcg acctttaaac gcgggatgta accggggtcg cgccccccta
    11461 aactcctccc tgacagaggc ccggataccc catcagggcc tctgttcttt ccgcctgtta
    11521 ccaacccgaa acgggcataa ataaacccgt ttcgggtagc atccaattgt taataattat
    11581 tattctcata tgagtaatgc ttttcggtaa gacgtgccat caggaggaag aatgatttct
    11641 ggcgcaccat ctcaggattc gctgttaccg gacaaccgcc acgcggctga ttaccaacaa
    11701 ttacgcgagc ggctcataca ggaactgaat ttaacgccgc agcagttaca tgaagagagc
    11761 aacctgatcc aggccggcct ggattccata agattgatga gatggttaca ctggtttcgt
    11821 aaaaatggct accgccttac ccttcgcgag ctgtatgccg cccccacgct ggcggcatgg
    11881 aaccagttaa tgctcagccg gtcgccggag aacgcggaag aagaaacgcc gcccgacgaa
    11941 tcatcctggc cgaacatgac cgaaagtacc cccttcccat tgacgccagt acagcacgcc
    12001 tacctgacgg gccgcatgcc ggggcagacg cttggcggcg tgggttgcca cctgtatcag
    12061 gagtttgaag gccattgtct gacggcgtcg cagctggagc aggccatcac gaccttgctg
    12121 caacgccacc caatgctgca tatcgccttt cgccccgacg ggcagcaggt ctggctaccg
    12181 caaccttact ggaacggcgt caccgttcat gatttacgcc ataacgacgc tgaaagccgc
    12241 caggcctatc tggacgcact gcgccagcgc ctgagccacc gtcttttacg cgtggaaatc
    12301 ggcgaaacgt ttgattttca gctgacgctc ttgccggaca atcgccaccg cctccatgtc
    12361 aatattgacc tgctgattat ggatgcctcc agctttacgc ttttcttcga tgagcttaac
    12421 gccctgctgg ccggagaatc gctgccggct atcgacaccc gctatgattt ccgctcgtat
    12481 ttgctgcacc agcagaagat caatcaacca ctgagagacg acgcgcgcgc ttactggctg
    12541 gcgaaagcat cgacgcttcc ccccgcgccc gtcttgccgc tggcctgcga acccgccacg
    12601 ctacgtgaag tccgtaatac ccgacgccgc atgattgtcc cggcaacacg ctggcacgcc
    12661 tttagcaacc gggccggcga gtatggcgtg acgccgacaa tggcactggc gacctgtttt
    12721 tctgccgtgc tggctcgctg gggcggcctg acgcgtctgc tgcttaacat caccttattc
    12781 gaccgccagc cgctgcaccc ggcggttggc gcgatgcttg ccgacttcac caatattctt
    12841 ctgctggata ccgcctgcga tggcgatacc gtcagcaacc tggcgcgtaa aaaccagctc
    12901 acgtttacgg aggactggga gcatcgccac tggtccggcg tcgaattact ccgtgaactc
    12961 aaacgccagc agcgctaccc ccacggcgcc ccggtggtat ttaccagcaa tctggggcgt
    13021 tccctctaca gcagccgcgc agaatcgccg ttgggcgagc cggaatgggg catctcgcaa
    13081 acgccgcagg tctggataga tcatctggcg ttcgagcatc acggcgaggt ctggctacaa
    13141 tgggacagca acgacgcgct gttccctccg gcgttagtcg aaacattgtt cgacacctac
    13201 tgccagttga ttaaccaact ctgcgatgac gaaagcgcct ggcaaaagcc gttcgcagat
    13261 atgatgcccg ccagccagcg cgcgatacgc gaacgggtca acgccaccgg cgcccccatt
    13321 cccgaaggct tgctgcatga aggcattttc cgtatcgctc tgcaacagcc gcaggcgctg
    13381 gcggtaacgg acatgcgtta tcagtggaat tatcatgagc tgacagacta tgcccgccgt
    13441 tgcgcgggca ggttaatcga gtgcggggtt cagcccggcg ataatgtggc tatcacgatg
    13501 tcgaaaggtg caggacaact tgttgcggtt ctggccgtcc tgctggctgg ggcggtttac
    13561 gttccggttt cgctggatca gcctgccgca cggcgcgaga aaatctacgc tgacgccagc
    13621 gtccggctgg tgctcatttg tcagcacgac gccagcgccg ggtcagacga tattcccgtc
    13681 cttgcctggc agcaggccat tgaggcggag ccgatcgcca acccggtagt acgcgccccc
    13741 acgcaaccgg cctacattat ctacacctcc ggctctaccg gtacgccgaa aggggtagtc
    13801 atttctcacc ggggagcgct taacacctgt tgcgatatca atacccgcta tcaggttggc
    13861 ccgcatgaca gggtgctggc cctctccgcc ctacattttg atttatcggt ttacgacatt
    13921 tttggcgtac tgcgcgcggg cggcgcgctg gtgatggtga tggaaaatca acggcgcgat
    13981 cctcacgcat ggtgtgagct gatccagcgc catcaggtca cgctctggaa cagcgtcccg
    14041 gcgctgttcg atatgctgct gacctggtgt gaaggtttcg ccgacgccac gccggaaaac
    14101 ctgcgcgcag tgatgctttc cggcgactgg atcgggcttg acctccccgc ccgttatcgg
    14161 gccttccggc cacaaggaca atttatcgcg atgggcggtg ccaccgaggc gtctatctgg
    14221 tctaacgcct gcgaaattca cgacgtcccc gcccactggc gctccatccc ttacggtttt
    14281 ccgctaacca accaacgcta ccgggtggtg gatgaacagg gccgggactg ccctgactgg
    14341 gtgccgggtg aattatggat tggcggcatt ggggtcgcgg aaggctattt caacgatccc
    14401 ctgcgtagcg agcagcaatt tttgacgctc ccggacgagc gctggtatcg caccggcgat
    14461 ctcggctgct actggccaga tggcacaatc gagttcctcg gtcgtcgcga caagcaggtc
    14521 aaagtcggag gatatcgcat cgagctgggc gaaatcgaaa gcgcgctcag ccagctggcg
    14581 ggggtgaaac aagcaaccgt tctggcgatc ggcgaaaaag aaaaaacgct ggcggcatac
    14641 gttgttcctc agggcgaggc tttttgcgtt accgatcatc ggaacccggc actgccgcag
    14701 gcgtggcaca cgcttgcggg aacgttgccc tgttgcgcca tctcgccaga gatctccgca
    14761 gaacaggtag ccgatttcct tcagcatcgc ctgctaaaac tgaagccggg tcacaccgct
    14821 ggcgccgatc ctctccccct gatgaactca ctcgctatcc agccgcgctg gcaggccgtg
    14881 gtggaacgct ggttagcatt tctggtgaca caacggcgac tgaagcccgc tgctgaaggt
    14941 tatcaggtct gcgctggtga agaacgcgag gatgagcacc cgcacttcag cggacatgat
    15001 ttaacgttat cgcaaattct tcgcggtgcc cgtaacgaac tgtcgttact gaacgacgcg
    15061 cagtggtcgc cggaaagcct ggcctttaac catccggcca gcgccccgta tattcaggaa
    15121 ctggcgacaa tttgccaaca gcttgcacag cgcttacagc acccggtgcg cctgcttgag
    15181 gtgggaaccc gcactggccg cgccgcagaa tcgctgttag cacagctcaa cgccggacag
    15241 attgagtatg tcgggcttga gcagagccag gagatgctgc tgagcgcccg gcagaggctc
    15301 gccccctggc ctggcgcccg tctgtccctc tggaatgcag acacgctggc ggcgcacgct
    15361 cactcggcgg acattatctg gcttaataac gccctgcatc gtctgctgcc ggaagatccc
    15421 gggctccttg cgacattaca acagcttgcc gttcccggcg cgctgctcta cgtgatggag
    15481 tttcgccagt taacgccgtc cgccctactc agcacgctcc tgttaaccaa tgggcagccg
    15541 gaggccttgc tgcataacag cgccgactgg gcggcattat ttagcgcggc cggcttcaac
    15601 tgtcagcatg gcgatgaggt cgcggggtta caacgcttcc tcgtacaatg tcctgacagg
    15661 caggtgcgcc gcgatccccg tcaacttcag gccgccctcg ccgggcgtct gccggggtgg
    15721 atggtgccgc aacggatcgt cttcctcgac gccttaccgc tgacggctaa cgggaaaatt
    15781 gactaccagg cgctgaagcg tcgtcatacc cctgaagcgg aaaacccggc cgaagcggat
    15841 ttaccccagg gcgacattga aaaacaggtt gccgccctct ggcagcaact cttatcaact
    15901 ggcaatgtca ccagagaaac cgacttcttc cagcaaggcg gcgatagcct gctggcgacc
    15961 cgtctgaccg ggcaacttca tcaggcaggt tatgaagcgc aattaagcga cctgtttaat
    16021 catccccggc tggcggattt tgccgccacg ctgcggaaaa ccgacgtccc ggtcgaacaa
    16081 ccattcgtcc actcccctga agatcgctac cagccctttg cgcttaccga cgtgcagcag
    16141 gcttacctag tggggcgtca gccgggcttt gccctgggcg gcgtcggctc acatttcttt
    16201 gttgaatttg aaattgccga tctggatctc acccggttgg agacggtctg gaaccgatta
    16261 atcgcccgcc acgatatgct acgcgccgtc gtgcgtgatg gacagcaaca ggtgctcgaa
    16321 cagacgcctc gctgggtaat acccgcacac atcctccata cgcctgaaga ggcgttgcag
    16381 gtgcgcgaaa aactggccca tcaggtactc aaccccgaag tctggccggt attcgatctc
    16441 caggtcggtt acgttgacgg gatgcccgcc cgcctgtggc tgtgtctgga taacctgtta
    16501 cttgacgggc tgagcatgca gattctgttg gcggagctgg agcacggcta ccgctacccg
    16561 caacagctgc ttccgccgct gcccgtcacc ttcagggatt atctgcaaca accctcgcta
    16621 cagtcgccca atccagattc tctggcatgg tggcaggcgc agcttgatga tattcctccg
    16681 gcgcctgcgt tgccgctgcg ctgcttgcct caggaggttg aaacaccgcg cttcgcccgc
    16741 ctgaacggcg cactggacag cacgcgctgg catcggctga aaaaacgggc ggctgacgcc
    16801 catctcaccc cgtcggccgt actgttgtcg gtgtggtcaa cggttctctc tgcatggagt
    16861 gcacagcctg agttcacgct taaccttacg cttttcgaca ggcgaccgct gcacccgcaa
    16921 atcaaccaga ttctgggcga tttcacctcg ctgatgctgc tgagctggca tcccggcgaa
    16981 agctggctgc acagcgcgca gtcactacag cagcggctga gccagaacct caaccaccgc
    17041 gatgtgtcag ccatccgcgt gatgcgtcaa ctggcgcaac ggcaaaacgt gccagccgtt
    17101 ccgatgcccg tcgtctttac cagcgcgctg ggctttgagc aggataactt cctcgcccgg
    17161 cgtaatctgc tcaaaccggt ctggggcatc tcccagacgc cgcaggtctg gctcgatcac
    17221 cagatttatg aatccgaagg cgaactgcgc tttaactggg attttgtcgc cgcgctgttt
    17281 cctgccgggc aggtggagcg ccagtttgaa cagtattgcg cattgctaaa ccgaatggcc
    17341 gaggatgaaa gcggctggca actgccgctc gccgcgctgg tgcctcccgt taaacacgca
    17401 gggcaatgcg cagagcgctc accgcgcgta tgccctgagc actctcagcc acacattgcg
    17461 gcggacgaga gcaccgtcag cctgatttgc gacgccttcc gcgaggtggt tggcgagtct
    17521 gtcacgcccg cagaaaactt ctttgaggcg ggcgcaacgt cgctgaatct ggtgcaactg
    17581 cacgttttgt tacaacgtca cgaattttcc accctgacgt tgcttgacct cttcacccac
    17641 ccttctcctg ctgccctggc cgattatctg gccggcgtcg ccacggtgga gaaaacaaaa
    17701 cgacctcgcc ctgttcgccg tcgtcagcgg cggatatagc gcgaagcaaa ctgattttcc
    17761 ccggaacgcc atcgcgaacg catggcgttc cattgacttt gtgaatctta ggaaacggga
    17821 ccgattatgg ataacttgcg cttctcttct gcgccgacag cagattccat tgatgcatcg
    17881 atcgctcaac actacccgga ctgcgaacct gtcgcggtta tcggctacgc ctgccatttt
    17941 cctgaatcgc cggatggcga aacgttctgg caaaatctgc tggaaggtcg tgaatgcagc
    18001 cgacgcttta cgcgcgaaga gcttctggcc gtcggtctgg atgccgccat cattgacgat
    18061 cctcattatg tcaatatcgg tacggtgtta gacaacgccg actgcttcga cgccaccctg
    18121 tttggctatt cgcgacagga agcggagtcg atggacccgc agcagcgcct gtttttgcag
    18181 gcggtctggc atgcgctgga acatgccggt tatgcccccg gcgccgtccc ccataagacc
    18241 ggcgttttcg cctcttcccg gatgagtacc taccccggtc gcgaagcatt gaacgtgaca
    18301 gaagtcgcgc aggtaaaagg tctgcaatct ctgatgggca atgataaaga ctatattgcc
    18361 acccgcgccg cgtacaaact caacctgcac ggcccggcgt tatcggtaca gaccgcctgc
    18421 tccagctcgc tggttgccgt gcatctggcc tgtgaaagcc tgcgcgcagg cgaatccgat
    18481 atggcggttg ccggcggcgt ggcgctctct ttcccccagc aggcaggcta ccgctaccag
    18541 cccggaatga ttttctctcc tgatggtcac tgtcgtccct ttgacgcctc ggctgagggc
    18601 acctgggccg gtaacggtct cggctgcgtg gtgctgcgtc gcctgagaga cgcgctgctg
    18661 tcaggcgatc cgattatctc ggtgatcctc tccagcgcgg tcaacaacga cggcaacaga
    18721 aaggtcggct ataccgcccc ttccgtcgca gggcaacaag cagtcatcga agaggcgtta
    18781 atgctggcgg ccatcgacga caggcaggta ggttacattg aaacccacgg caccggcaca
    18841 ccgctgggcg acgcgattga aattgaagcg ttacgcaacg tctatgcgcc tcgcccgcag
    18901 gatcagcgct gtgcgctcgg ttccgtgaaa agtaacatgg gccatctgga taccgcggcg
    18961 ggcattgccg gactgctgaa aaccgttctg gcagtcagtc gcgggcaaat tcctccctta
    19021 ctgaattttc acacccccaa cccggcgctg aaacttgaag agagcccctt taccataccg
    19081 gtgtcggcac aggcatggca ggacgaaatg cgctatgcgg gcgtctcctc ctttggtatt
    19141 ggcggcacca actgccatat gatcgtcgcc tcgctgcccg acgcgctcaa cgcgcgcctc
    19201 cccaatacgg atagcggcag aaaaagtacc gcgctgctgc tcagcgccgc cagcgacagc
    19261 gcgttgcggc ggctggcgac ggattatgcc ggggcgctga gagagaatgc ggatgccagc
    19321 tctctggcct tcacagccct gcacgcgcgc cgtctcgatc tccccttccg cctggcggcg
    19381 ccattaaacc gtgaaaccgc cgaggcgctc agcgcctggg ccggtgagaa atcgggggcg
    19441 ctggtttaca gcggccacgg cgccagcggc aagcaggtgt ggctgtttac cggccagggc
    19501 tcgcactggc gcactatggg tcaaacgatg taccagcact caacggcgtt tgccgacacg
    19561 ctggatcgct gtttttccgc ctgtagcgaa atgctcacgc cgtcactgcg cgaagcgatg
    19621 tttaaccccg attcggcgca gctggacaat atggcctggg cgcagccggc gattgtcgcg
    19681 tttgaaatcg cgatggcggc gcactggcgt gctgaaggac tgaagccaga cttcgccatt
    19741 gggcattccg tcggtgaatt tgccgctgcc gttgtctgcg gacactatac gattgaacag
    19801 gtcatgccac tggtttgtcg gcgcggcgcg ctaatgcagc agtgcgcaag cggcgcgatg
    19861 gtggcggtat ttgcagacga agacacgctg atgccgctgg ctcgccagtt tgagctggat
    19921 ctcgccgcca acaacggtac gcaacatacg gtattttccg ggccggaagc ccgtctcgcg
    19981 gtattttgcg ccacgctctc gcagcatgac attaactatc gtcgcctgag cgtaaccggt
    20041 gcggcgcact ccgctttact ggagccgata ctcgatcggt tccaggacgc ctgcgcggga
    20101 ctgcacgcgg agccggggca aataccgatt atttccacgc tcaccgccga cgtcattgat
    20161 gagtcaacgc tcaaccaggc ggattactgg cgccgacaca tgcgccagcc ggtgcgtttt
    20221 atccagagta ttcaggtggc gcatcagctc ggcgcccgcg tttttctgga gatggggccc
    20281 gatgcccagt tggttgcttg cgggcagcgc gaataccgcg ataacgcata ctggatagcc
    20341 agcgcccggc gtaacaaaga ggcgagcgat gtcctcaatc aggccctgct ccagctttac
    20401 gctgccggcg tcgccctacc gtgggccgac ctgctggcgg gcgatggaca acgtatcgct
    20461 gcgccatgtt atccgtttga tactgaacgt tactggaaag agcgcgtctc cccggcctgc
    20521 gagcctgccg acgcagcgct gtctgccggg ctggaggtgg cgagtcgcgc cgcgacagcg
    20581 ctcgatctcc ctcgcctgga tgcgcttaaa cagtgcgcca cgcgactgca cgccatctac
    20641 gtcgatcaac tggtacaacg ctgtaccggc gatgccattg agaacggcgt ggacgccatg
    20701 accatcatgc gccgtggacg tctgctgccc cgctaccagc agctactcca gcgcctgctg
    20761 aataactgcg tggtcgacgg cgattaccgc tgcaccgacg ggcgatacgt ccgcgcccgc
    20821 cccattgaac atcaacagcg ggaatcactg ctgacggaac ttgccggtta ttgtgaaggt
    20881 tttcaggcta ttcccgacac catcgcccgt gccggcgatc ggttatatga aatgatgagc
    20941 ggcgcggaag aaccggtggc gattatcttc ccgcaaagcg cctccgacgg cgtggaagtg
    21001 ctgtatcagg aattcagctt tggccgctat ttcaaccaaa tcgccgccgg ggtattacgc
    21061 ggcattgtcc agacgcgtca gccccgccag ccgttgcgta ttcttgaagt tggcggcgga
    21121 accggcggca ccaccgcgtg gctgctgccg gaactcaacg gcgttccggc actggagtac
    21181 catttcaccg atatctcggc gctgttcacc cgtcgcgccc agcagaaatt cgccgactat
    21241 gattttgtga agtatagcga gctggatctc gaaaaagagg cgcagtctca gggtttccag
    21301 gcacagtctt acgatcttat cgtggcagcg aacgtgattc acgccacccg ccatattggc
    21361 cgcacgctcg ataatctgcg ccccctgctc aagccgggcg ggcgcctgct gatgcgcgaa
    21421 atcacccagc caatgcgtct gtttgacttc gttttcggcc cgctggttct tccgctacag
    21481 gatctcgacg cccgcgaagg tgagttattc ctcaccaccg ctcagtggca acaacagtgc
    21541 cgccacgccg gattcagcaa agtggcgtgg ctaccgcagg atggcagccc gaccgccggg
    21601 atgagcgaac atatcattct cgccacgctg cccggtcagg cggttagcgc cgtaacattc
    21661 accgcgccat cagaacccgt gttggggcag gcgctgacgg ataacggtga ttatctcgcc
    21721 gactggtctg attgcgcagg tcagcccgaa cggtttaacg cccgctggca ggaggcatgg
    21781 cgtctgcttt cacagcgtca tggcgacgct ctgcctgtgg aaccgccccc cgtcgccgcc
    21841 ccggagtggc tggggaaggt tcgcttaagc tggcaaaacg aagccttttc ccgcggtcag
    21901 atgcgcgttg aagcccgtca tcctgctggc gagtggctgc cgctatcgcc cgccgcgcct
    21961 cttcctgcgc cgcaaacgca ttatcaatgg cgctggacgc ccctcaacgt cgccagcatt
    22021 gaccatccgc ttacctttag cttcagcgcc ggtacgcttg cgcgcagcga cgagctggcg
    22081 caatacggca tcattcacga tccgcacgcc tcttcacgac tgatgattgt tgaggagagc
    22141 gaggatacgc tggccttagc ggagaaagtg atagcagcgc tcaccgccag cgcagccgga
    22201 ttgattgtgg ttactcgccg cgcgtggcga gtcgaggaaa atgaagcact ctctgcatcc
    22261 catcacgcgc tatgggcctt gcttcgcgtc gcggccaacg aacagccgga acggttgctt
    22321 gccgccatcg atctcgccga aaacaccccg tgggaaacgc tgcatcaagg gttgagcgca
    22381 gtctcactat cacagcgctg gctcgccgca cggggtgaca ccctttggct cccttcactg
    22441 acgcccaata cgggatgcgc cgctgaatta ccggcaaacg tgtttaccgg cgatagccgc
    22501 tggcatctgg tgaccggagc gtttggcgga ttaggccgcc ttgccgtgaa ctggctcaga
    22561 gaaaaagggg cgcgacgcat cgccctgctg gcaccgcgcg tggatgagtc atggctacgc
    22621 gacgtggagg gcgggcagac gcgcgtctgc cgttgtgatg tgggcgatgc cgggcaactg
    22681 gccacggttc ttgacgatct ggcggccaac ggcggcattg ccggagcgat tcatgccgct
    22741 ggcgtattgg ctgacgcgcc cttgcaggag cttgatgacc accagctggc tgccgttttc
    22801 gcggtaaaag cgcaggcggc aagccaactg ttgcaaaccc tgcgcaacca cgacggacgc
    22861 tatcttattc tctactcttc cgctgccgcc accctcggcg cgccgggtca gagcgcccat
    22921 gcgctggcct gcggctacct ggacgggctg gcccagcagt tttccaccct tgatgcgccg
    22981 aaaacgctct ctgtcgcctg gggcgcatgg ggagaaagcg gtcgggcggc cacgccggaa
    23041 atgctggcga cgctcgccag ccgaggtatg ggcgcgttaa gcgatgccga aggctgctgg
    23101 cacctggaac aggcggtgat gcgcggcgcc ccgtggcgac tggcgatgcg cgtttttacc
    23161 gacaaaatgc ccccgttaca acaggctctg tttaacatca gcgccacaga aaaagccgca
    23221 acgccggtca ttcctcctgc tgatgacaac gcctttaacg gcagcctgag cgatgaaaca
    23281 gcggtgatgg catggctgaa aaagcggatt gcggttcagc taaggctgag cgatccggcg
    23341 tcactgcatc caaaccagga tctgttgcaa ctcggcatgg actcgctgct cttccttgaa
    23401 ctcagtagcg atattcagca ctacctgggt gtacgcatca atgcggaacg ggcgtggcag
    23461 gatctgtctc ctcatggact cacgcagctt atctgttcta agccagaggc gacgcctgcc
    23521 gcttcgcagc cggaagtatt gcggcacgac gccgacgagc gttatgcgcc cttccctttg
    23581 acgcccattc agcacgccta ctggctgggg cgaacccacc tcattggcta tggcggcgtc
    23641 gcctgtcacg tcctgtttga gtgggataaa cgccacgatg agttcgatct cgccatactg
    23701 gagaaagcat ggaaccagct catcgcacgc cacgatatgt tgcgtatggt ggttgatgcc
    23761 gacgggcagc agcgaatcct ggcgacaacg ccggagtatc acatcccgcg tgacgatctg
    23821 cgcgcgcttt ccccggaaga acagcgcatc gcactggaaa aacggcggca tgaactgagc
    23881 tatcgcgttt tgcctgccga ccagtggcct ctttttgagc tggtggtcag cgaaatcgac
    23941 gattgccatt accgtctgca tatgaacctc gaccttttgc agtttgatgt gcagagtttt
    24001 aaagtcatga tggacgacct ggcgcaggtc tggcgcggtg aaacgctggc gccgctcgct
    24061 attaccttcc gtgattatgt gatggctgaa caggcgcgcc gacagacatc ggcatggcac
    24121 gatgcctggg attactggca ggaaaaactg ccgcaactgc ccttagcgcc agagctgccg
    24181 gtggttgaga cgcccccgga aacgccacac ttcaccacct tcaaatcgac gatcggcaag
    24241 acagaatggc aggccgtgaa acagcgctgg cagcagcaag gcgtcacacc gtctgccgcg
    24301 ctgctcacgc tgtttgccgc cacccttgag cgctggagcc gtaccacaac atttacgctg
    24361 aacctgacgt tcttcaatcg ccagccgatc catccgcaaa tcaaccagtt gattggtgat
    24421 tttacctccg tcacgctggt tgattttaac ttctcagcgc cggtgacgtt gcaagagcag
    24481 atgcaacaga cccaacagcg cctctggcaa aacatggcgc acagtgaaat gaacggtgtt
    24541 gaggtgatcc gtgagctggg ccgcctgcgc ggatcacaac gtcaaccgct gatgccggta
    24601 gtgtttacca gtatgctggg gatgacgctg gaaggcatga ctatcgatca ggcgatgagc
    24661 catctgttcg gcgaaccctg ctatgtattc acgcaaacgc cgcaggtctg gctggatcat
    24721 caggtcatgg agagcgacgg cgagttgata tttagctggt actgcatgga caacgtgctg
    24781 gaacccggcg ctgccgaggc gatgtttaat gactattgcg ccatcctgca agccgtcatc
    24841 gccgcccctg aaagcctgaa gactctcgcc agcggcatcg ccgggcacat tccccgccga
    24901 cgctggccgc tgaacgcgca ggcggactac gacctgcggg atattgagca ggcgacgctc
    24961 gaataccccg gcatccggca ggccagagcg gaaataaccg aacagggcgc gttgacgctg
    25021 gatatcgtga tggccgacga tccgtcgcca tcagcggcga tgcctgatga gcacgaactt
    25081 acccaactgg cgctgccgtt gcctgagcag gcgcagcttg atgagctgga ggcgacctgg
    25141 cgctggctgg aggcgcgagc gctacagggg atcgcggcta cgctaaatcg tcacggcctg
    25201 tttaccacgc cggagatcgc ccatcgcttt agcgcaatag tacagacgct gtccgcgcaa
    25261 gcgtctcacc agcgtctgct gcgccagtgg ctacagtgtc tgacggaaag agagtggtta
    25321 atccgcgaag gtgaaagctg gcgctgccgc attccgctca gcgagattcc tgagcctcag
    25381 gaagcgtgcc cgcaaagcca atggagccag gcgctggcgc agtatctgga aacctgcatc
    25441 gcccggcacg acgccctctt ctccgggcag tgttctccgc tggagttgct gttcaacgag
    25501 cagcatcgcg ttaccgacgc gctgtatcgc gacaaccccg ccagcgcctg tctgaatcgc
    25561 tataccgcgc agattgccgc cttgtgcagc gcagaacgga ttctggaggt tggcgccgga
    25621 accgcagcca ctaccgcgcc ggtgctgaag gccacgcgga acacgcggca gtcgtaccac
    25681 ttcacggacg tctccgcgca gttcctcaat gacgccagag cccgtttcca tgatgaatcg
    25741 caggtgtctt atgccttgtt cgacatcaac cagccgctgg atttcaccgc ccacccggag
    25801 gcgggttacg acctgatcgt tgccgtcaat gtgctccacg acgccagcca tgtcgtccag
    25861 acgttgcgca gattaaaact gttgctgaaa gccggcggac gtttgctgat cgttgaagcg
    25921 acggagcgaa acagcgtatt ccagctggcg agcgtgggct ttattgaggg attaagcgga
    25981 taccgcgatt tccgccgccg ggatgagaaa ccgatgctca cccgctccgc atggcaggag
    26041 gttctcgttc aggccgggtt tgcaaacgag ctggcgtggc ccgcgcagga atcgtcgccg
    26101 ctgcgccagc atctgctggt agcgcgttcg cctggcgtaa atcgcccgga taaaaaagcc
    26161 gtgagccgct atttacagca gcgctttggc accggtctgc ccattttaca gatccggcaa
    26221 agagaagcgt tatttacgcc gctgcatgcc ccgtctgatg cgccgactga gccagccaaa
    26281 cccacgccag ttgccggggg gaatccggcg ctggaaaaac aggtggctga actctggcaa
    26341 tcgctgctgt ctcgccccgt ggcaaggcat cacgactttt tcgaactggg cggcgacagc
    26401 ctgatggcga caaggatggt cgcgcagcta aaccggagag ggattgccag ggctaacctt
    26461 caggatctgt tcagccattc gacgctgagc gacttctgcg cccatctaca ggcggctacg
    26521 tcaggagagg acaacccgat acccctttgc cagggcgacg gtgaggaaac cctgtttgtc
    26581 ttccacgctt cagacggcga tatcagcgcc tggctgccgc tcgctagcgc gttgaacagg
    26641 cgcgttttcg gcctgcaagc aaaatcgccg cagcgctttg ccacgctcga ccagatgatc
    26701 gatgagtatg tcgggtgcat ccgtcgtcag cagcctcacg gcccttatgt gctggcgggt
    26761 tggtcgtatg gcgcgtttct tgcggcgggc gccgcacaac gcctgtacgc caaaggcgag
    26821 caggttcgga tggtgttaat cgatcccgtg tgccgacagg atttctgttg cgaaaaccgg
    26881 gcggccctgc tgcgcctgtt agccgaagga caaacgcctc tggcactgcc cgaacatttc
    26941 gaccagcaga cgcccgacag ccagcttgcc gactttatca gcctcgctaa aacggccggt
    27001 atggtgtcgc aaaacctgac gctgcaagcg gcagaaacgt ggctcgacaa catcgcgcat
    27061 ctgctgcgtt tactgactga gcatacgccg ggcgaaagcg ttccggtccc ctgtctcatg
    27121 gtgtatgccg ccgggagacc cgcgcactgg acgccagcag aaaccgagtg gcagggctgg
    27181 ataaacaacg ccgacgacgc tgtgattgaa gccagccact ggcaaatcat gatggaagcc
    27241 ccccacgttc aggcttgtgc gcaacacatt acgcgctggc tttgcgcaac ctcaacgcaa
    27301 ccggagaaca cgttatgatg ccgtccgcct ccccaaaaca acgcgtactg attgtgggcg
    27361 ccaaatttgg cgaaatgtac ctgaatgcct ttatgcagcc cccggagggg ctggaactgg
    27421 tcggcctgct ggcgcaggga agcgcccgtt caagagagct ggctcatgcg tttggcattc
    27481 cgctgtatac ctcgccggaa cagataacca ggatgccgga tatcgcctgt attgtcgtgc
    27541 gttcgaccgt cgccggagga accggtacgc agcttgccag acactttctg acgcgcggcg
    27601 tacacgtcat tcaggagcat cccctccacc cggatgacat cagttcctta caaacgctgg
    27661 cgcaggaaca gggctgctgc tattgggtaa acacgtttta tccgcatacg cgcgcgggac
    27721 gcacatggct tcgcgatgcg cagcagctgc gtcgctgtct ggctaagacg ccgcaggtgg
    27781 tccacgccac caccagccga cagttgctct actccactct ggatttgttg ttgctggctc
    27841 tgggcgttga tgccgccgcc gtggagtgcg acgtggttgg cagctttagc gattttcact
    27901 gtctgagact cttctggccg gagggagaag cctgcctgct gcttcagcgc tatctcgatc
    27961 ctgacgatcc agatatgcat agccttatca tgcaccgcct gctgctgggc tggccggaag
    28021 gtcatctttc gctggaggcg agctacggcc ccgtcatctg gtcatccagc ctgtttgtcg
    28081 cagaccatca ggagaacgcc cacagcctct accgcagacc ggagatcctg cgcgatctgc
    28141 cgggtctgac gcgcagcgcg gcgcccctga gctggcgcga ctgctgcgaa accgtcgggc
    28201 cggagggcgt cagctggttg ctacaccagt tgcgcagcca tctggcgggc gaacatccac
    28261 cagcggcctg ccaaagcgta catcagatcg cgttatcacg tttatggcag cagattttac
    28321 gtaagaccgg caatgcggag atcagacgcc tgacgccgcc gcaccacgac cggttagccg
    28381 gtttctacaa cgatgatgat aaggaggcgc tgtgacgcaa tctgcaatgt gcatcccgct
    28441 gtggcccgcc cggaacggca atactgcgca tctggtcatg tgccctttcg ctggcggcag
    28501 cagtagcgcg tttcgccact ggcaagctga gcaactggct gattgcgcgc tttctctggt
    28561 gacctggccg gggcgcgatc gccttcgcca tctggaaccg ctcagaaaca ttacacaact
    28621 ggcggcactg ctggcgaacg agctggaagc atccgtatcg cctgacacgc cgcttttact
    28681 cgccgggcac agcatggggg cgcaggtggc gtttgaaacc tgccgacttc tggagcaacg
    28741 ggggcttgcg ccacaaggac tgattatttc cgggtgccat gccccgcatc tgcattctga
    28801 acgccagctc agccatcgcg atgatgccga ctttatcgct gagctgatag acattggcgg
    28861 atgttctcct gaactgcggg aaaaccagga attaatgtcg ctgtttcttc ctcttctgcg
    28921 cgctgatttt tacgccaccg agagctatca ctacgactcg cccgacgtct gtccgccgct
    28981 gcgcacgcct gcgctgttat tgtgcggcag ccacgatcgc gaagcctcct ggcagcaggt
    29041 cgatgcctgg cgtcagtggc tgagccacgt tacaggcccg gtggtgattg acggcgatca
    29101 tttctatccc attcaacaag cccggtcctt ttttacgcag attgtccgcc attttcccca
    29161 cgcattttct gcaatgaccg cgtggcaaaa acagcccagt acttcagaaa ggtgacgcat
    29221 gaattcttcc tttgaatctc tgattgaaca gtatccctta cccattgccg aacagttgcg
    29281 ccactgggcg gcccgttatg cctcgcgaat tgccgtcgtt gatgcaaagg ggtcgttaac
    29341 ctacagcgcg cttgatgcac aagttgacga acttgccgca ggtctgtcat cactgggttt
    29401 gcgttcgggg gagcatgtaa ttgtgcagct tcccaacgac aacgcgtttg ttaccctgct
    29461 gttcgccttg ttaagactgg gcgttatccc cgtgctggcg atgccctcgc aacgggcgct
    29521 ggatatcgac gcgctgattg agctggcgca acccgtcgct tacgttattc acggggaaaa
    29581 ccacgcagag ctggcccgac agatggcgca caaacacgcc tgcttgcgtc atgttctggt
    29641 cgctggagag accgtgagcg acgattttac gccgctcttc tcccttcacg gtgagcgaca
    29701 ggcatggccg cagcctgatg tttccgccac cgcgttgttg ttgctctcag gcggcacaac
    29761 cggcacgccc aaactcatcc cgcgccgaca tgccgactat agctataact tcagcgcttc
    29821 tgctgaactg tgcggcatca gccaacagag cgtgtatctc gccgtcctcc cggtggcgca
    29881 taactttccg ctggcctgcc ccggtattct gggaacgctt gcctgcggcg gaaaagtggt
    29941 gctgaccgac agcgccagct gtgatgaggt gatgccttta atcgcgcagg aaagagtgac
    30001 tcacgtcgcc ctggttccgg cgctggcgca attatgggtg caggccaggg agtgggaaga
    30061 cagcgacctt tcgtcgctgc gcgtcattca ggcaggcggc gcccggctcg acccgacgct
    30121 tgctgagcag gttatcgcca cctttgactg taccctgcaa caggttttcg gtatggcgga
    30181 aggcctgctc tgttttaccc ggctggacga tccgcatgcc accattctcc acagccaggg
    30241 gcgcccgttg tcccctctgg atgaaatccg catcgttgat caagacgaga acgacgtcgc
    30301 gccgggcgaa accgggcaat tgttaacgcg cggcccttat accatttcgg gctattaccg
    30361 cgcccctgcc cacaacgcgc aggcctttac cgcgcaaggg ttttaccgca caggcgacaa
    30421 tgtcaggctg gatgaggtgg ggaacctgca cgttgaggga cgcataaaag agcagatcaa
    30481 ccgcgccgga gaaaaaatag ccgcggctga agtggaatcg gcactgctgc gtttagcgga
    30541 agtgcaagat tgcgcggtgg tcgccgcgcc ggacacgctg cttggcgagc ggatttgcgc
    30601 gtttatcatc gcgcagcagg tgccaactga ctatcagcag ttgcgtcaac aactgacccg
    30661 tatggggctc agcgcgtgga aaattcctga ccaaatcgag tttctggacc actggccgct
    30721 caccgccgtc ggcaagatag acaaaaaacg cctgacggct ctcgccgtcg accgttatcg
    30781 ccattctgcc caataagcgc aaaccgaccc gaaacaggtt gaaataaacc cgtttcgggt
    30841 agcaccacta ttagaaatag ttatcatttt caattcacca ttgtcggtat ttttggcgtt
    30901 tcgccgtctt acagggactc acaacaatga aaatgacacg gctttatcct ctggccttgg
    30961 ggggattatt gctccccgcc attgctaatg cccagacttc acagcaagac gaaagcacgc
    31021 tggtggttac cgccagtaaa caatcttccc gctcggcatc agccaacaac gtctcatcta
    31081 ctgttgtcag cgcgccggaa ttaagcgacg ccggcgtcac cgccagcgac aaactcccca
    31141 gagtcttgcc cgggctcaat attgaaaata gcggcaacat gcttttttcg acgatctcgc
    31201 tacgcggcgt ctcttcagcg caggacttct ataaccccgc cgtcaccctg tatgtcgatg
    31261 gcgtccctca gctttccacc aacaccatcc aggcgcttac cgatgtgcaa agcgtggagt
    31321 tgctgcgagg cccacaggga acgttatatg gcaaaagcgc tcagggcggg atcatcaaca
    31381 tcgtcaccca gcagccggac agcacgccgc gcggctatat tgaaggcggc gtcagtagcc
    31441 gcgacagtta tcgaagtaag ttcaacctga gcggccccat tcaggatggc ctgctgtacg
    31501 gcagcgtcac cctgttacgc caggttgatg acggcgacat gattaacccc gcgacgggaa
    31561 gcgatgactt aggcggcacc cgcgccagca tagggaatgt gaaactgcgt ctggcgccgg
    31621 acgatcagcc ctgggaaatg ggctttgccg cctcacgcga atgtacccgc gccacccagg
    31681 acgcctatgt gggatggaat gatattaagg gccgtaagct gtcgatcagc gatggttcac
    31741 cagacccgta catgcggcgc tgcactgaca gccagaccct gagtgggaaa tacaccaccg
    31801 atgactgggt tttcaacctg atcagcgcct ggcagcagca gcattattcg cgcaccttcc
    31861 cttccggttc gttaatcgtc aatatgcctc agcgctggaa tcaggatgtg caggagctgc
    31921 gcgctgcaac cctgggcgat gcgcgtaccg ttgatatggt gtttgggctg taccggcaga
    31981 acacccgcga gaagttaaat tcagcctacg acatgccgac aatgccttat ttaagcagta
    32041 ccggctatac caccgctgaa acgctggccg catacagtga cctgacctgg catttaaccg
    32101 atcgttttga tatcggcggc ggcgtgcgct tctcgcatga taaatccagt acacaatatc
    32161 acggcagcat gctcggcaac ccgtttggcg accagggtaa gagcaatgac gatcaggtgc
    32221 tcgggcagct atccgcaggc tatatgctga ccgatgactg gagagtgtat acccgtgtag
    32281 cccagggata taaaccttcc gggtacaaca tcgtgcctac tgcgggtctt gatgccaaac
    32341 cgttcgtcgc cgagaaatcc atcaactatg aacttggcac ccgctacgaa accgctgacg
    32401 tcacgctgca agccgcgacg ttttataccc acaccaaaga catgcagctt tactctggcc
    32461 cggtcgggat gcagacatta agcaatgcgg gtaaagccga cgccaccggc gttgagcttg
    32521 aagcgaagtg gcggtttgcg ccaggctggt catgggatat caatggcaac gtgatccgtt
    32581 ccgaattcac caatgacagt gagttgtatc acggtaaccg ggtgccgttc gtaccacgtt
    32641 atggcgcggg aagcagcgtg aacggcgtga ttgatacgcg ctatggcgca ctgatgcccc
    32701 gactggcggt taatctggtc gggccgcatt atttcgatgg cgacaaccag ttgcggcaag
    32761 gcacctatgc caccctggac agcagcctgg gctggcaggc gactgaacgg atgaacattt
    32821 ccgtctatgt cgataacctg ttcgaccgtc gttaccgtac ctatggctac atgaacggca
    32881 gcagcgccgt cgcgcaggtc aatatgggtc gcaccgtcgg tatcaatacg cgaattgatt
    32941 tcttctgatt attgtaaaag ggataccgaa aaggtatccc ttttacacca ctagttaaaa
    33001 ccagtaactc agcagagtcg caaaaatatt aatccatagt gattatttaa acaatgaaat
    33061 tgcgattagg at
//
LOCUS       DAJOTB010000038        33046 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJOTB010000038 DAJOTB010000000
VERSION     DAJOTB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.038"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..162)
                     /locus_tag="OHN28_001788"
     CDS             complement(<1..162)
                     /locus_tag="OHN28_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCQ2297193.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSP"
     gene            complement(368..1072)
                     /locus_tag="OHN28_001789"
     CDS             complement(368..1072)
                     /locus_tag="OHN28_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCQ2297194.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1209..1661)
                     /gene="moaE"
                     /locus_tag="OHN28_001790"
     CDS             complement(1209..1661)
                     /gene="moaE"
                     /locus_tag="OHN28_001790"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCQ2297195.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1663..1908)
                     /gene="moaD"
                     /locus_tag="OHN28_001791"
     CDS             complement(1663..1908)
                     /gene="moaD"
                     /locus_tag="OHN28_001791"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCQ2297196.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1901..2386)
                     /gene="moaC"
                     /locus_tag="OHN28_001792"
     CDS             complement(1901..2386)
                     /gene="moaC"
                     /locus_tag="OHN28_001792"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCQ2297197.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2389..2901)
                     /gene="moaB"
                     /locus_tag="OHN28_001793"
     CDS             complement(2389..2901)
                     /gene="moaB"
                     /locus_tag="OHN28_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCQ2297198.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2923..3912)
                     /gene="moaA"
                     /locus_tag="OHN28_001794"
     CDS             complement(2923..3912)
                     /gene="moaA"
                     /locus_tag="OHN28_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCQ2297199.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3900..4044)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4309..5217
                     /gene="yvcK"
                     /locus_tag="OHN28_001795"
     CDS             4309..5217
                     /gene="yvcK"
                     /locus_tag="OHN28_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCQ2297200.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5255..7276)
                     /gene="uvrB"
                     /locus_tag="OHN28_001796"
     CDS             complement(5255..7276)
                     /gene="uvrB"
                     /locus_tag="OHN28_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCQ2297201.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7855..8532)
                     /gene="bioD"
                     /locus_tag="OHN28_001797"
     CDS             complement(7855..8532)
                     /gene="bioD"
                     /locus_tag="OHN28_001797"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2297202.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8525..9280)
                     /gene="bioC"
                     /locus_tag="OHN28_001798"
     CDS             complement(8525..9280)
                     /gene="bioC"
                     /locus_tag="OHN28_001798"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCQ2297203.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9267..10421)
                     /gene="bioF"
                     /locus_tag="OHN28_001799"
     CDS             complement(9267..10421)
                     /gene="bioF"
                     /locus_tag="OHN28_001799"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCQ2297204.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10418..11458)
                     /gene="bioB"
                     /locus_tag="OHN28_001800"
     CDS             complement(10418..11458)
                     /gene="bioB"
                     /locus_tag="OHN28_001800"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCQ2297205.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11545..12834
                     /gene="bioA"
                     /locus_tag="OHN28_001801"
     CDS             11545..12834
                     /gene="bioA"
                     /locus_tag="OHN28_001801"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCQ2297206.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12893..13369
                     /locus_tag="OHN28_001802"
     CDS             12893..13369
                     /locus_tag="OHN28_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCQ2297207.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13521..14804
                     /locus_tag="OHN28_001803"
     CDS             13521..14804
                     /locus_tag="OHN28_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCQ2297208.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14945..17206)
                     /locus_tag="OHN28_001804"
     CDS             complement(14945..17206)
                     /locus_tag="OHN28_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCQ2297209.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17389..18822)
                     /locus_tag="OHN28_001805"
     CDS             complement(17389..18822)
                     /locus_tag="OHN28_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCQ2297210.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18898..19950)
                     /locus_tag="OHN28_001806"
     CDS             complement(18898..19950)
                     /locus_tag="OHN28_001806"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCQ2297211.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20134..21087
                     /locus_tag="OHN28_001807"
     CDS             20134..21087
                     /locus_tag="OHN28_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2297212.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21128..22123)
                     /gene="pgl"
                     /locus_tag="OHN28_001808"
     CDS             complement(21128..22123)
                     /gene="pgl"
                     /locus_tag="OHN28_001808"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCQ2297213.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22278..23096
                     /gene="ybhA"
                     /locus_tag="OHN28_001809"
     CDS             22278..23096
                     /gene="ybhA"
                     /locus_tag="OHN28_001809"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCQ2297214.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23097..24155)
                     /gene="modC"
                     /locus_tag="OHN28_001810"
     CDS             complement(23097..24155)
                     /gene="modC"
                     /locus_tag="OHN28_001810"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCQ2297215.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24158..24847)
                     /gene="modB"
                     /locus_tag="OHN28_001811"
     CDS             complement(24158..24847)
                     /gene="modB"
                     /locus_tag="OHN28_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCQ2297216.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24847..25620)
                     /gene="modA"
                     /locus_tag="OHN28_001812"
     CDS             complement(24847..25620)
                     /gene="modA"
                     /locus_tag="OHN28_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2297217.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(25787..25936)
                     /gene="acrZ"
                     /locus_tag="OHN28_001813"
     CDS             complement(25787..25936)
                     /gene="acrZ"
                     /locus_tag="OHN28_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCQ2297218.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26065..26853
                     /gene="modE"
                     /locus_tag="OHN28_001814"
     CDS             26065..26853
                     /gene="modE"
                     /locus_tag="OHN28_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCQ2297219.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26921..28393
                     /gene="modF"
                     /locus_tag="OHN28_001815"
     CDS             26921..28393
                     /gene="modF"
                     /locus_tag="OHN28_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCQ2297220.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            28656..29672
                     /gene="galE"
                     /locus_tag="OHN28_001816"
     CDS             28656..29672
                     /gene="galE"
                     /locus_tag="OHN28_001816"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCQ2297221.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29682..30728
                     /gene="galT"
                     /locus_tag="OHN28_001817"
     CDS             29682..30728
                     /gene="galT"
                     /locus_tag="OHN28_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCQ2297222.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30732..31880
                     /gene="galK"
                     /locus_tag="OHN28_001818"
     CDS             30732..31880
                     /gene="galK"
                     /locus_tag="OHN28_001818"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCQ2297223.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31874..32914
                     /gene="galM"
                     /locus_tag="OHN28_001819"
     CDS             31874..32914
                     /gene="galM"
                     /locus_tag="OHN28_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCQ2297224.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
BASE COUNT         8059 a         8843 c         8295 g         7849 t
ORIGIN      
        1 cggtgaatca tattttgttg ccgtaatttc gagatactta gcgccccagg ccagccctgc
       61 ggttgccacc aaacccaaag cggtaatgag ccatatgccg tttaatatcc tggcttcgtg
      121 ctgaaaatct aatttattgc tattttgcga gtatgattcc attcttttag ttgtccttag
      181 aaaaaacatt aatattcacc atcaataaat ttaaaaccac gcatacaaaa tacttttgca
      241 tttatggata cagaaaacgc ttccgctcgt ccataaggca tattttatac ctggtacttg
      301 ttaagtgtcg tttttaataa gaaaaaaaat cccgacacat ggccgggata ttgatgatga
      361 aaaagaatta acggcggttg ccgaagatcc gcagcaacat caggaacagg ttgatgaagt
      421 ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcgag
      481 tatcaatctg ctcacccata tttttcagtt tctgggtgtc atacgccgtc aatccgacaa
      541 agacaatcac gccgatgtag gtgactgccc acatcaatgc ttcgcttttc agccagaagt
      601 taaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccgc
      661 ttagatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc ccggcagtaa
      721 cgacgaaagt actggcgata gaagcagcgg tatagacaat gaaaatactg gaaagcgtaa
      781 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
      841 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
      901 agacacggtt agtaaacaac agctccatca cggccgcgga attagccgca taccaggcaa
      961 caaatgctgt cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt
     1021 gcaagccagc ccggggttgt acgattgaat cagaacgtgg gaatctgtcc atgactttct
     1081 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
     1141 agcgtaaacg gatgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
     1201 acaataaact accagcgttt tgccgcctgc tgatcgctct cccgagcttc gacccagcgg
     1261 tcgccttccg gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata
     1321 ataaactgcc cggcttcaaa cgcactgctg cgatgcgcac tggtaacacc gacaaaaaca
     1381 atttcatcgc ccggccataa ttcaccaatg cggtgaatta ccgtgacgcg ccccagcggc
     1441 cagcggttac gcgcttcatc aacgatttct gccagtgctt tttctgtcat tcccggatag
     1501 tgttcgaggg ttaatgcttt gacgctgtcg cccaggttat ggttgcgcac cttaccggta
     1561 aaggtgacta ccgcaccgtc ttcgtcacgc tcagccagcc acgggtactc ttctcctacg
     1621 ctgaacggct gcgggccgac aacaattttg gtttctgcca tcttaacctc cggttaccgg
     1681 cgggaagaaa gccacttcgt cgccgtcagt cagcgaatgg tcaaaactca ccagcgtctg
     1741 gttgacagca gccagcaatt tgccatcttc cagcgccaga gcccagcgat cgctctgcgc
     1801 agccagatgc tggcgtaacg cttcaacggt tgggaaatcc gcagccactt cggttgcatc
     1861 agttcccacc aactcgcgca cctgggcgaa aaaaagaacg ttaatcatcc gcttccacct
     1921 taaagtcacc cgacttgccg ccgctctttg ccagcaaacg taccggacca atcaccatat
     1981 ctttttgcac cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta
     2041 acgcttccat ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgtaccc
     2101 gattgtgctc cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcgggtgac
     2161 acagcgggat cagatcccag gtgcgttttg ccgcctgaat accggcgata cgcgcagtgg
     2221 caaatacgtc gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca
     2281 tggtgacaaa ggcttcggcg cgcgcttcac gcacggtttc cgctttggcg gagacatcga
     2341 ccatgtgcgc ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa
     2401 atgtggatgg aaattacacg gacgcgtacg ggcatccagc tgcggcgcaa tgatattttc
     2461 ccatgcggta cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttagc
     2521 gacgcccgct accgcacgag attgcaacgt ggaagtgcca atctcttcga acgacaacat
     2581 ccggaacact tcaccaaaac cttcaacttc acggtcgaac aacggcagca gcgcttcggg
     2641 agcctgatct ccttccgtca ggccagtacc acccgtaatc aacaccactt gtacatcgtc
     2701 gctggcgatc cacgcagaga cctgagcgcg aatagcgtag cggttttctt tcacaatggc
     2761 tttatcgaca acgtgatggc ccgcttcttg cgccgagtcg cgaagatagt gaccggaggt
     2821 atcgtcttct tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa
     2881 ttcagtgctt acctgactca tctgatctct ccttttgacg ttttagccac caatgtacga
     2941 taagttttgc gtaataccgg tgttattttg atgcaggaaa tgggtctgtt ttttctcccg
     3001 cagcgccgct gaaatacgcg cttccagcgc ctgttgctgg gtatcgtttt ccagcagatc
     3061 gcgcaggtta acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac
     3121 gcgcaggcgg ttgcaagtgg cgcagaaatc tttttcatac ggcatgataa ggccaatctc
     3181 tccggcgtaa tccggatgac aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg
     3241 gtggatccag ccgcgacgca gtaactcgtc acgcagaacc tgaccagaga tgtgatgctt
     3301 acggaagagc tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatggg
     3361 acgatgctgg atccagttca gaaaggtgtc gagctggtga tgattcacat cacgcatcag
     3421 cacggtattg actttgacct tctcaaaacc ggcctcaaat gcagcatcga ttcctgccat
     3481 gacctgattg aatttatcct gcccggtaat ggcatgaaac tggcgagcgt caaggctatc
     3541 aacgctgacg ttgatgccag taagtccagc atcgcgccag ttcgccacat cgcgctccag
     3601 acggtaacca ttggtggtga ccgctatctg gcgaatagcg tcgttttctc gcacagcggc
     3661 gatgatatcg gtaaagtcgc ggcgtaaaga tggctcacca cctgtcagac ggactttttc
     3721 ggtgcccaga ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg tcaggaagcc
     3781 tttattggtg acgccgctcg gcttgtaacc atccggcagg cagtaggtgc aacgaaagtt
     3841 acacacatca gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag
     3901 ttgtgaagcc atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct
     3961 ggtggcaatc tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca
     4021 gaggctagag tgtttagtgg ttaagccgat actagcgtgt aaatgagatt tttaccatcc
     4081 acactttcgc tatgtaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata
     4141 agggaaaatg gcggttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc
     4201 tttagaggta gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttcct
     4261 cgcaatttgc gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt
     4321 acgctggctg atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt
     4381 ctctcatcac tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac caccgataac
     4441 ggtggctcga cggggcgtat tcgccgctca gaaggcggca ttgcctgggg cgatatgcgc
     4501 aactgcctca atcagctgat aactgaaccc agcgtcgctt ccgcaatgtt tgaatatcgt
     4561 ttcggcggta atggcgaact ttccgggcac aaccttggaa acctgatgtt aaaggcactg
     4621 gatcacctta gcgtgcgacc tcttgaagcg atcaatctga ttcgaaatct gctgaaagtg
     4681 gacgcacatt tgattccgat gtcggagcat cctgttgatc tgatggcgat tgacgatcag
     4741 gggcatgaag tttacggcga ggtcaatatc gaccagttaa ctgcgccgat tcaagagtta
     4801 ttgttaacgc cgaatgtacc cgcaacgcgt gaggcggttc acgctatcag tgaagcggat
     4861 ctcatcatta ttgggcctgg cagtttttac accagcctga tgccaattct gctgctgaag
     4921 gaaatcgcac aggcattacg ccgcacgcca gcgccgatgg tctatatcgg caatctgggg
     4981 cgtgagttga gtttacctgc ggctaatctg aagctggaaa gcaagctggc aattatggag
     5041 cagtatgttg gtaaaaaggt aattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg
     5101 gcggtgaaag agcggattgt gattcaggaa gtgctggagg ccagcgatat tccgtatcgt
     5161 catgaccgcc agttgttgca tagcgcgctg gaaaaggcat tacaggcttt aggttaacac
     5221 gaagccggaa gaatattccg gcttcgtcat cttgtcacga cgcggcaata aacagctcac
     5281 gcaactgatg caactggtca cgaatttgcg ccgcttcttc gaactccaga ttctgcgcgt
     5341 gttgcatcat caacccttcc agttcatgga tcttctgctg taacgcttta ggcgacatat
     5401 ccatcggcac attatccggc tcaacaatcg ggcgcgattt tcctctgccc ttcgctttgg
     5461 ttttggcaat gttctgcccc agcgccagga tatcgaccac tttcttgttc aagccttgcg
     5521 gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt tcggtttcgc
     5581 caatcgcttt cgccattgat ggggtgatct tatcgccgta gagaatcgct ttaccgttaa
     5641 cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc aggaagcctt
     5701 ctttgtcagc gtcgaggatc gccaccagcg aaacctccgg catatccaga ccttcgcgca
     5761 gtaagttgat ccctaccaat acgtcgaact cacccagacg caagtcgcgg ataatctcca
     5821 tacgttcgac ggtatcgata tctgagtgaa gataacgcac gcgctcaccg tgttcttcga
     5881 gatattcagt gagatcttcc gccatccgct tggtcagagt tgtaaccagt acgcgttcgt
     5941 taattgccgc tcgctgacga atctccgata gaagatcatc cacctgtgtc gctaccggac
     6001 gcacttcaat aatcgggtcg agtaagcctg ttggacgcac aacctgatcc accacatcgc
     6061 caccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg gtttgcggcg
     6121 ctaatgcttc gaactcttca aatttcagcg ggcggttatc cagcgctgat ggcaagcgga
     6181 agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg ccgccaattt
     6241 gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcagcaggc aggtaatcaa
     6301 acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag tagttctcaa
     6361 tccccgaaca gtagcctagc tcgttcatca tctccagatc aaactgggta cgctgggtca
     6421 gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttacgtctg gcggccagct
     6481 cttctttgat ctcctccatc gcctggacga tgcgctcgcg tggcgtgacg tagtgcgttt
     6541 tcgggtagat ggtaaaacgt ggaatagtgg aaacaatctg cccggtcagc gggtcaaata
     6601 acgacaatcg ttccacctcc tcgtcaaaca gctccacgcg aagtgcaatg tcatccgatt
     6661 ctgccgggaa gatatcaatc acctcgccac gaacacggaa agtaccacgc tggaatgctt
     6721 gatcattacg ggcatattgc agctccgcca gtcggcgcag gatcgcgcgc tgatcgataa
     6781 tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg cccagaccat
     6841 aaatcgcgga aacagacgcc accacaacca catcacgtcg ctccagcatc gctttggtgg
     6901 cggataaacg catctgttcg atatgttcgt ttaccgaggc atctttctca ataaaggtgt
     6961 cggaactcgg tacataggcc tccggctgat agtagtcgta gtaggagacg aaatattcca
     7021 ccgcgttttc tgggaagaat tctttcattt cgccatacag ctgggccgcc agcgttttgt
     7081 tgggcgcaag taccatggtt gggcgctgaa ggtcagcaat gacattggca atggtgaagg
     7141 ttttcccgga gccagtcacg ccaagtaacg tctggtgcgc caggccatct tccagcccct
     7201 cttcgagacg tcgaatcgcc tccggctgat cgccagaagg tttaaaagcg gaattcagtt
     7261 tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta tgtaatttta
     7321 ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt catcaccata
     7381 atatttctgt tacagcgtaa actccgctgt caatcatgaa caagatttac tctgcggcga
     7441 gataaaactc cggccttacc gggttatccc caaagcaacg gcttttttaa catttgtcaa
     7501 gatgattgat gacagtttta tggcaagaga tgcctgttca gtgactgcca ttgcaattat
     7561 ctaacctgtt aaaaaataaa agatatttct ttgagctgtc tttaacgcca attcgcgtgc
     7621 aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat ggttatccac
     7681 aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa ttcccacatt
     7741 caccgcatga tgcggaggta aaattttttt cgtgattttt ttagaattta attggttaaa
     7801 ttgcagtcaa tcggagacac gatctcgctc gcaatttaac caattacagg atgactacat
     7861 taaggcaaga ttgatgtact ttccggttgc cgcattttct ggattttctg caagccaggg
     7921 aatttcgccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat attcagcgtg
     7981 acgttttccc ggaggcgtga catcgttcgc cacccacccc gccagagtca gtccggcgtg
     8041 tagtattgcc tgcgcagtca acatcgcgtg attaatacag ccgagtttca cgcccacaac
     8101 cagtatcact ggcagttgtt cctgtgttac ccaatccgca aaagtgaaag tgtcagaaag
     8161 cggcgtaaac cagccgccag caccttccac taacacccag tcagcctgtt gttcaagcgc
     8221 gcgtaatccg gcgctcatta ccaatgcttc tatcggcctg ccctcttgcg cgctgatgat
     8281 atgcggcgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat ccagctgcag
     8341 gctactgttg cgctgtaacg ccagcgcgtc actattacgt aagcctttcg gggtcttttc
     8401 gctgccagag gccaccggtt tgtaacctgc cgtccggtag cctgctgcct ttgcggcttg
     8461 taaaagcgcg cagctggcga cagttttccc cacttcggta tccgttccgg tgacaaaata
     8521 acatttactc acgagcaatc actcccaaaa aaagatgata cgtcagagga tatcgcccct
     8581 gttgttgcgg ccaggccagt tgcaatcgct gcaactgcga acgcgttaat attcgcgggt
     8641 cacgcccttc atgaagatgc gtggcaccga tgcctttcag cgaacgcatg gcactgagcg
     8701 catcatcaaa ccacagcgtg atgggctgaa tataatgttg ataatgcacg gcgtttagcg
     8761 actgttcgat ttcatctggc ggtaaaaagc gattagcatg cggacgctcg tccaccgcct
     8821 gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac gcgaccacgc
     8881 ctccggggcg caccacccga tacagctcgc ggagtgccgt ggataaatta ccgcaccact
     8941 gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc agggattcga
     9001 tatctcccgc cagataatgg tctgcggcat ccttctggcg tgcctgaaca agcattggcg
     9061 gagagagatc taacgcagtc acctgcgcgt gacgctctcg ccagcggcgg ctcatccagc
     9121 ctggtccaca acccgcatcc agtacgcggg tgtatttacg ctgtgggagc attgccagta
     9181 aggcgtcagc actctggcgc tgtagatcgg cgtgttgctc atagtgtgcg gctgcccgtc
     9241 caaatgccgc agcaatggct tgtttattaa ccgttgccat gcagcacctc cagcagacgg
     9301 tcgatatcct gcatttcatg cgctgcggtt agcgttaagc gcagtcgcgc agtaccagcg
     9361 ggtacggttg gcgggcgaat tgccgtgacc caacagcctt gctggcgcag tttttctgcc
     9421 agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct ccatgaatca
     9481 gcaagcgtaa acggcaaatc ctgcactccg gcacgaaaac gcgttatgag tgccgccagt
     9541 ttttcgcgcc gtgcatcccc ctcatcactg cgaatgaccg ccagcgacgc acgtaatgcc
     9601 tgcgcctgag cgggcggcat actggtgctg tagataagat ggcgagcgaa ttgcagcaga
     9661 taatccgcca ccgtattgga gcaaagcacc gctgccccgc tgacgccaaa tcctttgcca
     9721 aacgtcacca ccagcaattc tggttttacc ttttgcagcc agcagctgcc acgcccctgc
     9781 tccccgataa cgcccgtgcc gtgggcgtca tcgaccatca accagccatc gtgctgttgc
     9841 gttacctgct ggatttcctc cagtggcgca ctatcgccgt ccatgctgaa cacgccttct
     9901 gtcactacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag atgagtgaca
     9961 tcgttatgag caaaacggcg aagcggcgac gggcttaaac ttgcagcctc cagcaatgag
    10021 gcatggctaa gccggtcggc aacaatacgg tcctctttcg ccatcatcgc ggcaatgact
    10081 gcctgattag cagcaaaacc agagataaac agcagtgccc gcgaatagcc cagccactcg
    10141 gccagttctt cttccagcgc ctgatgcgcc acgctataac cgctgacgtg accggagccg
    10201 ccgctaccga tgccaaattg ctccgcaccc tgcttccagg cacggataat ttgcggatga
    10261 tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc atccaccacc
    10321 agccagcgtc cggctccttg cgccaccgga taacggcgac gcagggcatc ggcagcacgc
    10381 cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc gcgttgtaat
    10441 attcgtcagt gtccggggtc atcagcgcct gctccagacg ctgctgttgt tcgttatcgc
    10501 ccgccagcac ggcagtttgc tgcggattta gccccagttt gcggaacagt tgcaggtctt
    10561 tatcttcttc cggattcggc gtggtcagca gtttgcaacc gtagaaaatc gagtttgcgc
    10621 cagccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg gcagaaaggc
    10681 gtacgtaaga ggtcggcatc atgatccgcg caactgcaat ggtgcgaata aaatcaaagg
    10741 catcgacatc atcgttatcc gccagcggcg tgcctttcac cttcaccagc atgttgattg
    10801 gtacgctttc cggcggcgtt ggcaggtttg ccagttgcag caataatccg gcgcgatctt
    10861 ttactgtttc gcctaagccc acaatgccgc ctgaacagac tttgatcccg gcgtcgcgca
    10921 ctttttccag cgtatcgagg cgttcctgat aggtacgcgt ggtgatgata ttgccgtaaa
    10981 actccggcga ggtgtcgagg ttgtggttgt agtaatccag cccggcattc gccaggcgct
    11041 gcgcctgaga ttcactcaac gtgcccagcg tcatacacgc ctccagcccc atcgctttta
    11101 ccccctgcac catttgttcc aggtacggca tatcgcgttc gtggggattc ttccacgccg
    11161 cgcccataca gaaacgcgtc gatcccgccg ctttcgcttt gcgcgccgac tccagcacct
    11221 gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag ctttgcgggc
    11281 aatatttgca atcttccgga caagctccgg tcttaatcga cagcaaagtg ctgacctgca
    11341 cctgacgagg atcgaaatgc tgacgatgca cctgctgcgc ttcaaacagc agatccagca
    11401 acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg tgagccatgg
    11461 ggcttctcca aaacgtgttt tttgttgtta attcggtgta gactcgtaaa cctaaatctt
    11521 ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga ccaacgccat
    11581 atctggcacc catacacatc catgacctcc ccgctgccgg tttatccggt ggcaagcgcc
    11641 gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat gtcgtcctgg
    11701 tgggcggcga tccacggtta caatcacccg cagcttaatg cggcgatgaa gtcgcaaatt
    11761 gatgctatgt cgcatgtgat gtttggcggt atcacccatg cgcccgccat tgagctgtgc
    11821 cgcaaattgg tggcaatgac gccgcaacca ctggagtgcg tttttctcgc ggactccggc
    11881 tccgtagcgg tagaagtggc gatgaaaatg gcgttgcagt actggcaggc caaaggcgaa
    11941 gcgcgccagc gttttctaac cttccgcaat ggttatcatg gcgatacctt tggcgcgatg
    12001 tcggtgtgcg atccggacaa ctcaatgcac aacctgtgga aaggctatct gccagaaaac
    12061 ctgtttgctc ccgccccgca aagccgcatg gatggcgaat gggatgagcg cgatatggtg
    12121 ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cggcggtgat cattgaaccg
    12181 attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa acgaatccgc
    12241 aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac cggatttggt
    12301 cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggatat tttgtgtctc
    12361 ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cgctcaccac gcgcgaggtt
    12421 gcggaaacca tcagtaacgg cgaagccggc tgctttatgc atgggccaac ttttatgggc
    12481 aatccgctgg cctgcgcagc agcaaacgcc agcctggcga ttctcgaatc tggcgactgg
    12541 cagcatcagg tggcggctat tgaagcgcag ctacgcgagc aacttgcccc cgcctgtgac
    12601 gccgaaatgg ttgccgatgt gcgcgtactg ggggcaatcg gcgtggtcga aacctctcgt
    12661 ccggtgaata tggcagcgct gcaaaaattc tttgtcgaac agggtgtctg gatccggcct
    12721 tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca gttgcagcgt
    12781 ctgaccgcag cggttaatcg tgcggtgcag gatgaaacat ttttttgcca ataacgggaa
    12841 gtcagcgtga gggtttctgg ctacactttc tgcaaacaag aaaggagggc tcatgaaact
    12901 catcagtaat gatttacgcg atggcgataa gctgccgcat cgtcatgtct ttaacggcat
    12961 gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc ctgtgggaac
    13021 gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca actggctccg gctggtggca
    13081 ctgggtagtt gttaatttac ccgcggatac ccgcgtatta ccgcaagggt ttggatctgg
    13141 tctggtagca atgccagacg gcgttttgca gacgcgtacc gactttggta aaaccggata
    13201 cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg ttcacgcgct
    13261 ggatgtagaa cgtattgatg tcgatgaagg tgccagcggc gcgatggtcg ggtttaacgt
    13321 tcattttcac tctctggcga gtgcctcgat tactgcgatg tttagttaat cactctgcca
    13381 gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt tgtcttttta
    13441 ccttcccgtt tcgctcaagt tagtataaaa aagcaggctt caacggattc atttttctat
    13501 ttcatagccc ggagcaacct gtgaacacat tttcagtttc ccgtctggcg ctggcattgg
    13561 cttttggcgt gacgctgacc gcctgtagct caacaccgcc cgatcaacgt ccttctgatc
    13621 aaaccgcgcc tggtacctct tctcgcccga ttctgtcggc aaaagaagcg cagaatttcg
    13681 atgctcaaca ctattttgca tccctgacac caggtgcggc agcgtggaat ccttccccga
    13741 ttaccctgcc tgcgcaacct gactttgttg tcggcccggc gggtactcaa ggtgtaacgc
    13801 ataccacgat tcaggcggcg gtagatgcgg caattatcaa gcgtaccaac aagcgccagt
    13861 atattgccgt gatgcctggt gagtatcagg gaacggtgta tgtccctgcc gctccgggtg
    13921 gaattactct gtacggtaca ggtgaaaaac cgattgatgt gaagattggg ctttcccttg
    13981 atggtggcat gagccctgcc gactggcgtc acgacgtcaa cccgcgcggc aaatatatgc
    14041 caggtaaacc ggcgtggtat atgtacgata gctgccagag taaacgcagc gacagtatcg
    14101 gtgttctctg ctctgcggtc ttctggtcac aaaacaatgg cctgcaactg caaaacctga
    14161 ccatcgaaaa cacgctgggc gatagcgtag atgcgggtaa ccatccggcg gtggcactgc
    14221 gtactgatgg cgacaaagtg cagatcaata acgtcaacat tctcggtcgt cagaacacct
    14281 tctttgtcac caacagcggt gtgcagaacc gtctggaaac gaatcgccag ccgcgtacgc
    14341 tggtgaccaa cagctatatt gaaggggatg tggatatcgt ttctggtcgc ggcgcagtgg
    14401 tgttcgataa caccgaattc cgcgtggtga actcccgtac ccagcaagaa gcgtatgtgt
    14461 ttgcaccggc tacgctgtcc aacatttact acggtttcct cgccgtaaac agccgtttca
    14521 atgcttccgg tgatggcgtg gcgcaactgg gccgctcgct ggatgttgat gccaatacca
    14581 acggtcaggt agtgatccgt gatagcgcca tcaacgaagg ttttaacaca gccaaaccgt
    14641 gggctgatgc ggtgatctct aatcgcccgt ttgcgggtaa caccggcagc gttgatgata
    14701 acgacgaagt acaacgcaat ctgaatgaca ctaactacaa ccgcatgtgg gaatacaata
    14761 accgcggcgt gggtagcaaa gtggttgcag aggcgaagaa gtaggagctg ttaactatgt
    14821 gccggatgcg gcgtaaacgc cttatccggc ctacagttcg atacgatttg tcggtcggat
    14881 aagatgcgcc agcatcgcat ccgacaataa gtgccggatg cgacctacat tcacatggcg
    14941 ctttttacat ctgacggttt ttattgaagt taatcaaact acccgccttg ataatctcgc
    15001 gttcttcggc agtcagactt tccatataga gcgtgatttc cgttaccggt gcatcttcat
    15061 ggatcacata acctttaaac gtcgttccag gattatccag cgccgcttta atgccaggga
    15121 tgtaaatgta atcccccact tcaaaggttg gcgcttccgc catctgcagc ggtaacatcc
    15181 cccagttgat gacgttagaa cgatagcgtt tagtcgcgta ctcctcggca atattcgcca
    15241 gaccgccaat cacacgctga caactcgccg cctgttcacg cgcagaacca tcgcctggtt
    15301 tcaccgcata gaccatgctg ccaatttcag tttgcagcgg atcaacatgt tcctgaccag
    15361 caatctgctt aatgcgcgca aacacctctg ccatctcgct gacattcccc gccagacgct
    15421 gattttccag ctcagcagtc gctttacttc tgccaacata acctggatcg cggcgtgaca
    15481 gggtaaactc cgccagacca atcggatttg aacgataaga agaggtttca ccggaaggaa
    15541 tcagttcgtc ggtggtggtc acttcgtcga ggatcttcga gcacactttg aggacgatat
    15601 tgtcagtcag cgcacccaat tccggccagt ctttaatgtt cggtccgtaa atcagcggtt
    15661 gctgggtcgc ccctttcaca aagccttgat agacgcgatt tttatacggc gttacatcga
    15721 aggcgtactc cggcacgttg tcccagcaat cgagttcgct ggcagaggtt aaatagccac
    15781 cgtttgccgc agtcgcagcg atagaacgag cgtccatcaa cgccaccgct gacatctgcc
    15841 cattagctgg cttagagcct tcgcggttcg ggaagttacg cgtggtgtgg cgaatactca
    15901 aaccgttgtt gattggcgta tcgcccgcgc caaagcatgg gccgcagaac gcggttctga
    15961 tgattgcgcc tgcgccaatc aaatctgcga ccacaccttt tttggcgaga tccataaaca
    16021 ccggctgtga tgacgggtaa accgctaacg agaaggtgtc attgccgcag gattgaccgc
    16081 gcagcgcatt cgccgccgcg atgacgtttt catagttacc gccagaacag cccgcgataa
    16141 tcccctgctg cactttcagg cgaccatttt ccactttatc cagcagcgag agtttggctt
    16201 taccgtgcgc cacgcgttcg gactcaattt caatctcacg cagaatgtcg gtcaggttct
    16261 ggttcagcgt gtcgatttca tacacgttgc tcgggtggaa tggcagcgca atcattggtt
    16321 tgatggcgct taaatcaacg ctgatgcagc catcgtagta cgccattggt tgagggttaa
    16381 gttggcagta atcctggccg cgaccgtgca gcgccagcca gttatggact tcttcatcgg
    16441 tttgccagac agaacttaaa caggtcgttt cagtggtcat cacgtcaacg ctgttacgga
    16501 aatcggtaga gagcgctgca acaccgggac ctacgaactc catcactttg tttttgacgt
    16561 aaccgttttt gaacacggca ccgatgatag ccagcgccac atcctgcggc cccacatacg
    16621 gcgcaggttt tccggtcaga tgcaccgcaa ccacgcccgg atagtcgata tcccaggtgt
    16681 cattaagcag ttgttttacc agctcgccgc cgccctcacc gactgccatt gtccctaatg
    16741 cgccgtaacg ggtgtggctg tctgatccga ggatcatttt gccgccgcca gccatcattt
    16801 cacgcatata ttgatggatg accgcaatat gcggtggcac aaaaataccg ccataacgct
    16861 gggccgccga taaaccaaaa acatggtcat caccgttaat ggtgccgccg acggcgcaga
    16921 gtgagttatg gcagttggtc agcacatacg gcagcgggaa acgttccata ccggacgctt
    16981 tagcggtctg tacaatacca acaaaggtaa tatcgtgaga ggctaatgaa tcaaacttaa
    17041 ttttaagttt atccatattt ccggacgtat tatgagaaga gagaatagac caggcaatag
    17101 tgcctttttt ggcttcttct tttttaattt cgccggtgaa atgttcttcg gcaattattt
    17161 cgttattact ggcgagaaac acgccttttt cagataactt gatcatcaaa tgctccagat
    17221 atctgcgcac cggaaagtgc gcagaaagat aataaatgga tatactctaa ataattcgag
    17281 ttgcaggaag gcgacaagcg agtgaatccc cagtcactta cataagtaag tgactggggt
    17341 gagcgaacgc agacgcagca catgcaactt gaagtatgac gagtatattt acagcatgtt
    17401 ccagccgatc agcatattcc accaccacac gccgagggtg atatgcacca ggaaggttaa
    17461 tatcgtcagt accgcaccga ccaaccacca ggatttaata tcgttgtaac ccacgccaaa
    17521 gatgaccgga cctgccgcgc cgccatagtg agtaaccatg ccgccatagg agttagagaa
    17581 caacagtgcc agcgcggtta acattaacgg tgcgccggag acattcgcca gcatggcaaa
    17641 taccggtaac atggcaacga tataggcact accagaagcg aagaaataac gcacgatgat
    17701 gctgaggaaa ataataacga agaaagcaac gttcccgtga ccatcaaatg ccaggttatt
    17761 tttaaagact tcagctaacc attcgaagaa tttaactttc gataataagg agcttaagcc
    17821 gataataccg ccgtaccaga ttaaggtatt ccagccgcct ttatttttaa ccacgtcttc
    17881 ccaggtaacg atacccagca gcagcatagt tgccataaca acgattgcca cggtggattc
    17941 atcaaccccc agagacttac tgaaaatcca gcccagcagc gccagcacaa agacaccgag
    18001 cagcattttt tcgcggattt tcatcggtcc cagttcggca aggcccgctt tagcgatggt
    18061 tttgttatcc accttcttaa tttctggtgg atacatggtg taaatcacca gcggggtgac
    18121 cagcagcata atgatgcccg gcaatccggc ggctaacgcc catccacccc agctaatttg
    18181 caggtgcaga atgtcgttga tcattttcag cgccagaatg ttccccgcca ttgcggtaaa
    18241 gaacatatag ctggtggttt tggtgaccat gtaaatggac atcatcaggt aatgtccaac
    18301 acgacgcgga cttttttccg gttctgatcc caaagccact gccacgctgt tgatgatcgg
    18361 taacacgatg ccgcccgcac gcgcggtgtt agacggtgtt gccggagcca gtaccagatc
    18421 gagaaatacc gtaacgtaac ccagacccag ggtagtgcta ccaattttac caatcagcag
    18481 ataggcaata cgtttaccta agcctgtggt tacaaacgcg gcgcttaaag taaacgcaga
    18541 aaacaccagc caggtggtac cggaagagta accgcttaat acagcggtgg ttttaaattc
    18601 cccacctgat aagttaccca ccactaccat cgatgcggcg acggcaatta acagtacgac
    18661 aggttccggg aaaggcttga taaccagccc cacaatggcc gccaggtaaa taccaaaaag
    18721 cacccacgcc agttcgctta atcctgccgg agctggcata aagccaataa tacatgggat
    18781 tgctaatatc agaattagct tccataacga tttcttattc atttaatatc ctgttctttc
    18841 tgtatgactc atacagaatt cctttattga ttgtcattat cacgacaaca aaatttttca
    18901 gggaagataa acttcaccgg aaaatatttt tctggtcgtc cgaataacag atgcgcgtaa
    18961 cgtcgtggca tcctgacctt cattacttaa atgaacgtcg agcgcaccac tggggtgttc
    19021 aatattgata ttgccgtatc ctacagaagg gacgatttgt cgggtgacgg taccttccaa
    19081 tgcacaacta ctggaaatag caatagcacc ggttatcgcc aaagcgcgat ggcaagaatg
    19141 cggcataaaa taacgcacat taattgcccc gcctttctgc gctggagaaa taagtacagg
    19201 tttagggata accatattac tgacatcgcc taaacccatt gctttacccg cttgtagacg
    19261 gatagattca atgcgggcta ataatgcttt gtcggcatcc agctctgccg gtaattcata
    19321 gcctgttttt cccagatatt cagcaggaat aatgacaact ggcatcgcca tatcgataca
    19381 ggtcaccggg acatcgtcaa aataatcaat ctgattatcc gtcgggaaga cttttccggt
    19441 tttcgttccg gcggcattca ggaaagtgag cgcaaccggt gcggcagtac ccggtacgcc
    19501 gtcaattctg gcgctaccct cgtactcgac aacaccattt ggcgtttgca catcagcttc
    19561 gatgaacgta cccgtattga cgttgcggat acgtacgcgg gtaactggcg aagtcgctgc
    19621 aatcaaacca ttttcaatgg cgaatgcccc aacgccagac agcatattgc cgcagttagg
    19681 cgtggtatcg acacgttgct catggacgat aacttgagca aacagataat cgacatctgc
    19741 acgcggatcg ctggagcggc taataatggc gactttactg gtcagcggat taccgccgcc
    19801 aataccgtca atttccagat cgttaccgga acccataatt gccatcaata ttttatcgcg
    19861 ctgcgtttga tcttcgggta gatgttccgc taacaggaac gcgcccctcg aggttccacc
    19921 tcgcatcatc acgcagggta tttttttcat aataatgccc tcaatttaaa aacataactt
    19981 attgcgatat ggttacatta agggcaaagc atctctaatg actttagctc aacgattaat
    20041 aacatttttt cattaattta agttggctaa atgcagttaa aatttttaac tgccagtcac
    20101 ccaaaatcgt cgcttccatt ttccggagta ataatgaagc atgaattatc aagtatgaag
    20161 gcatttgtca tactggcaga gtccagttca tttaacaatg ctgctaaatt actcaatatt
    20221 acgcaacctg cattaacgcg aagaataaaa aagatggagg aagatttaca cattcaacta
    20281 tttgagcgta caactcgcaa ggttacgtta acaaaagcag gaaaaaggtt gctcccggag
    20341 gcgcgggaat taataaaaaa attcgatgaa acgcttttta gtattcgcga catgaatgct
    20401 tatcatcgag gtatggtgac gttagcgtgt attccgaccg cggtatttta ttttttacca
    20461 ttggcaatcg gtaaatttaa tgagctgtat cccaatatta aagtgcggat tctggaacaa
    20521 ggcacgaata attgcatgga atcggtgctg tgtaacgaat cagactttgg cataaacatg
    20581 aacaacgtca caaattcatc cattgatttt accccactgg tcaacgagcc gttcgtgctg
    20641 gcctgtcggc gtgaccatcc attggccaaa aagcagcttg tagaatggca ggagttagtg
    20701 ggttacaaaa tgattggcgt acgctcctcc agcggcaacc gactgttgat agaacaacag
    20761 ctagccgaca agccctggaa gctggactgg ttttacgaag tgcgtcatct ttcgacgtca
    20821 ttagggctgg tggaggcggg actggggatt tcggcgcttc ctggtctggc aatgccccat
    20881 gcgccctatt cttcgattat tggtatccca ctggtggaac ccgttatacg tcggacattg
    20941 gggattattc gccgcaaaga tgccgtgctt tctccggcag ctgaacgctt ttttgccttg
    21001 ctgattaatt tgtggactga cgataaagac aatttgtgga ccaatatcgt cgaacgccag
    21061 agacatgcgt tacaggagat aggttaatac accggcgcag gagagactgc gccggttaaa
    21121 tcagcggtta gtgcgcgttg accactaccc acattggccc ctgcccaacc gcatagcggc
    21181 ctttttcatg cagtagcccc tgctcgccaa caatttcgta taccgagatg tggtgagatt
    21241 tttgcccggc ggcaatcaga tacttgccgc tgtgatcaac attgaagccg cgcggctggg
    21301 tttccgttgg ctggaagcct tctttactca acacgctgcc atcttccgaa acgctgaaaa
    21361 cggtaatcag actggcggta cggtcgcagg cgtataaatg acgaccatcc ggggtgatat
    21421 gaatatcagc cgcccaacgg gtgtcggaga agttttccgg catcatatcc agcgtctgga
    21481 cacattcgat attgccgtgc ggatctttca gttcccagac atccactgag ctgtttaact
    21541 cattgacgca atacgcatat tgttcgtttg gatggaatac catatgacgc gggccggccc
    21601 cttcaacggt agtcacttcc gcagggtcct gcgccaccag atgaccatca tcgctgaccg
    21661 taaacaggca aatgcgatcc tgctttaatg ccggaaccca caacgtacgg ttgtccggtg
    21721 agatattggc ggaatggcaa ccgtccagcc cctcgaccac atcgacgacg cccactggca
    21781 ggccatcttc cagacgcgtt acgctcacgt tacccgcatt gtaagaaccg acaaagacaa
    21841 actgcccctg gtgatcggtg gaaatatgcg tcgggctacc cggcagcgca gactctgcag
    21901 caaaggtcag cgcgccatca tccggtgcaa tacgatacgc cagaacacga aactccggac
    21961 ggacaccaac gtagaggtaa cgtttgtccg ggctgaccac catcggctgc acctgccccg
    22021 gcacatcgac aacctgtgtc agcgtcagtg cgccttcatg attcaggttc cagacgtgaa
    22081 tttgctggct ctcagggctg gcgatataaa ctgtttgctt catgaatgct cctttgcatt
    22141 tagcaactga ctgtaacagc taaaattagt cgcttttggc ggtgaatgct gaattaaaac
    22201 gcagaatttt gtccgatagt ggtatcggtg taccattcgc gcagacgaaa attcttaacc
    22261 ttaatctgga atacgccatg accacacgcg tgattgctct cgacttagac ggcaccttat
    22321 tgaccccgaa aaagaccctg cttccatcat cgatagaagc cctggcccgc gctcgcgaag
    22381 caggctatcg attaatcatc gtcacaggtc gccatcacgt cgctattcat cctttttatc
    22441 aggcgctggc gctggataca cctgctattt gctgtaatgg cacctatttg tatgattatc
    22501 atgcaaaaac cgtgctggaa gcggacccaa tgcccgttaa taaagccctg caactcattg
    22561 agatgctgaa tgaacaccac attcacggtc tgatgtatgt ggatgatgcg atggtctatg
    22621 agcacccgac cggacatgtc attcgcacgt ctaactgggc gcaaaccctg ccgccggaac
    22681 agcgtccgac tttcacacaa gtcgcttctc tggctgaaac ggcgcaacaa gttaacgccg
    22741 tatggaagtt cgccctgacg cacgatgacc tgccgcaatt gcagcatttt ggtaagcatg
    22801 tcgaacatga actgggactg gagtgtgaat ggtcctggca cgatcaggtt gatattgcac
    22861 gcggcggcaa cagcaaaggt aaacgtttga cgaaatgggt tgaggcgcaa ggctggtcga
    22921 tggaaaacgt cgtggcgttc ggcgataact tcaatgatat tagtatgctg gaagccgctg
    22981 gtacaggcgt ggcgatgggc aacgccgatg acgcagtaaa agcgcgcgcc aacattgtga
    23041 ttggtgataa caccaccgac agcattgccc agttcattta tagccacctg atttaatcag
    23101 gcggttatcg acacactttt aatttgcgca tacagccaca ggccaggttt gatcgccagt
    23161 tcatccctgg cccacgggct gatacgcgcc cacagcgttt tacagccgac ttccagctcc
    23221 acttccacct ggccgttgtc gtcataacta ttaacaactt ttgcccgcaa tacgttacga
    23281 atgctggttt gctgcggcgg ttgtaaaacc agtgagacat cggaagcctg aatgcggatg
    23341 cgtagcgcag cttgcagcgg ttcgtccagc ttattgaccc acaaatgctg atcgcccagc
    23401 gccagcgcgg tcatcgcgta atgcggatga tgctcaagta ctgtcacttt cagaatgcta
    23461 ctttgttgct ctttcggtag ccacggattc atcacgctac taccccagac ttcttccagc
    23521 gcgccaaagg ctttcacctg accgttttcc agtaccatca ctctgtctgc cagatggagg
    23581 atctcatcca gcgaatggct gacatacaac atcggaatgt tgatttcccg cgtcagacgt
    23641 tgcagataag gcaacagttc gcgtttacgc ggaatatcca gtgatgccag cggttcatcc
    23701 agcaacagca attccggtgc tgtcagcaaa gcccgaccaa tcgccacgcg ctgtttttcg
    23761 cctccggaca ggctgcctgg taaacgatca agcaacggtt caatgcctaa aagcgccacc
    23821 agcttatcga actgattgac catgcttttc gccatgccgt agcgcaaatt gccacgcact
    23881 ttataatgcg gaaacagccg cgcatcctga aacacatagc ctacgcgacg cttttctggc
    23941 gtcaggcaga tacctttatc ggcgtcattt agtacccgac cattgaggac aatccgccct
    24001 ttttgcggac gtgtcagccc actgatggcg ttaatcagcg aggtttttcc ggcaccagag
    24061 acaccaaaaa tagcggtgat gccattggcg ggcagcgttt cattaatcgt caggcaatgg
    24121 ttgcccaacg tctgggaaaa attcagttcc agcatgatta gcgccccgcc cgctcacggc
    24181 tgattctggc cagccattct gaaatcaaca gggagatcat cgccagcgca atagaaataa
    24241 tacacagtct cgccgcgcca ctttccccgc caggggtctg gatcagggta tacatggcag
    24301 aaggaatggt ccgcgtttca ccaggaatgt tcgacacaaa ggtgatggtt gcgccaaatt
    24361 caccgagcga acgagcgaat gccagtaccg taccaacaat aattcccggt aaggtcagcg
    24421 gtaacgtgat agtaaagaaa acgcgccagc gcccggcccc cagtgttctt gcggcctgct
    24481 ccagtttgat atcaacacct tccagcgcca gacgaatcgc ccgcaccatc agcggaaaag
    24541 acatgacagc ggcagcgaga accgcaccac gccagctaaa cgcgaaagaa agaccaaacc
    24601 agtcatacag acgttcaccg ataaatccgc gccgtcccat cgaaactaat aataggtaac
    24661 cgacgaccac gggcggtaac accagcggta gatgcagtac gctgtcgagc agagctttgc
    24721 ccggaaacgt gcaacgtacc agtaaccagg caaaaaagat cccaaacggc aggctaaaca
    24781 gcacagccag ggaagaaact ttcaggctta ataaaactgc ctgccattct ggatcggtca
    24841 gtatcattac ttggttgtaa atccgtaacg tttaaagatt tccgcagcct gcggcccctt
    24901 cagataatca taaaatgctt tcactgtcgc attgttatgc ccttccacaa cagcaaccgg
    24961 atattccact tttttatgtg aatcttccgg gaaggtggca accactttta cccctttgct
    25021 ggcaactgcg tcagaaccgt aaacaatacc cagcggcgct tcattgcgtt cgaccaacgc
    25081 cagcgcgcca cgaacatctt ccgccggagc cagtttcggg gaaagcgtat cccatgcgcc
    25141 cagtttttgc agtgcttctt tcgcataaat gccagcaggg acatgttccg gatcaccaac
    25201 cgcgaggcga ccgccattca gcagcgaagt ccagttggtt ttgctgtcga tggtgaaatc
    25261 tttctgctcg ctggctttcg gtgctacaac gaccaggcta ttgccgagca gtgtctggcg
    25321 cgaagccgta tcgatcgctt ttttatcaac cgcataatcc atccatttct gatcggcaga
    25381 aataaacaga tccgcaggcg cacccgcttc aatctgacgg gccaaagtag atgacgaagc
    25441 gaaagaagaa accacatcca cgcctttctc ttttttatac tgcgtagcaa tgtcctgcat
    25501 tgcgttagtc agtgatgcgg cggcgaacac cgtgattttc ccttcatctg ccagtgcatt
    25561 gccagcaaca gcgaaagaga gtgccgcccc ggtaaacaag ttcaaccatt tacgagccat
    25621 tggtaacccc ttaatctaac gttatgtagg cgtcaatata acggcaagtt cggaattttc
    25681 ccagcagtta ttgctaacct ttgtgaggta gatgagaaaa aatatcggca gaaaaagcag
    25741 gaagttgaga aaaagaaaat gcccgactaa gcgggcattc agggaatcaa tgattttgtc
    25801 cgggctggtc ttttttacca acaccagaaa agatgttgaa tacttcacca agaccgtaaa
    25861 tcagacccag gatgatggcc atcacgacag gtaccatgat tacggcgaat accagacttt
    25921 ttaataactc taacatggtc aactccagat atagtcatga gactattcta accgctaagc
    25981 acagaaaagc actccccttt tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa
    26041 taagactttt gccaggacat tgttatgcag gccgaaatcc ttctcaccct taagctccaa
    26101 caaaaattat tcgccgaccc gcgccgcatt tcgctactaa aacacattgc gctttccggt
    26161 tccattagcc agggagcgaa agatgccggt atcagctata aaagcgcctg ggatgccatt
    26221 aacgagatga atcagttaag tgagcatatt ctggttgagc gcgcaacagg cggtaaaggt
    26281 ggaggcggcg cagtactgac ccgctatggt cagcgactga ttcaactcta tgacttactg
    26341 gcgcaaatcc agcaaaaagc ctttgatgtg ttaagtgacg atgacgccct gccgctgaac
    26401 agcctgctgg ccgcgatctc acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc
    26461 ggtaccatca ccgctcgcga tcatgatgac gttcaacagc atgttgatgt cttactggct
    26521 gatggaaaaa cacgcctgaa agtcgcaatt accgcacaaa gcggcgcgcg tctggggctg
    26581 gatgaaggca aagaagtgtt gatattgcta aaagcgccgt gggtaggtat tactcaggac
    26641 gaggcggtcg cgcaaaacgc tgacaaccaa ttaccgggta ttattagtca tattgagcgc
    26701 ggcgcagagc agtgcgaagt attaatggcg ctacccgacg ggcaaacact gtgcgccaca
    26761 gtgccggtaa atgaagcgac ttctcttcag caagggcaga atgtcacggc ctactttaat
    26821 gccgacagcg tgattatcgc cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac
    26881 gtatccctgt cagtaatcgc tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt
    26941 gcaaggcacg tttcgtcttg gcgacacaaa aacgctgcaa ttgcctcggc taacgttaaa
    27001 cgcgggtgat agttgggcgt ttgtcggttc gaatggaagc gggaaatcgg cgctggcccg
    27061 cgcgctgtcg ggggaacttc cgcttttgaa aggtgaacgg caaagccagt tttcccacat
    27121 cactcgtctc tccttcgagc aattgcaaaa gctcgtcagc gacgaatggc agcggaataa
    27181 caccgatatg cttagtcctg gcgaagatga caccgggcgt accacggctg agatcattca
    27241 ggatgaagta aaggatactt cgcgttgcat gcaactggcg cagcagttcg gtattaccgc
    27301 cctcctcgac cgacgcttta aatacctttc cactggcgag acgcgaaaaa ccctgctgtg
    27361 tcaggcgctg atgtcggagc ctgacttgtt gatccttgat gaaccgtttg atggcctgga
    27421 tgttgcctca cgtcagcatc tggctgagct actcgcctcg ttacatcagt ccggtattac
    27481 tctggtactg gtgctcaatc gcttcgatga gatcccggaa tttgtccagt ttgctggcgt
    27541 gctggcggat tgcacgttag cggaaactgg cgctaaagaa gaactgctcc aacaagcact
    27601 cgttgcgcaa ctggcgcata gcgaactgct tgaaggtgtg caaatgccgg agccggatga
    27661 accttcagca cgtcacgcct tacccgccaa cgaaccgcgc attgtgctga acaatggcgt
    27721 ggtttcttat aacgaacgcc ccatccttaa taaccttagc tggcaggtga atccaggcga
    27781 acactggcaa attgtcgggc caaatggcgc gggaaaatcg acgttattaa gcctgattac
    27841 tggcgatcat ccgcaaggtt acagcaacga tttgacgctt ttcggacgac gtcgcggcag
    27901 cggcgaaacc atctgggata tcaaaaagca tatcggttac gtcagcagta gtttgcatct
    27961 ggattaccgg gtcagcacta ctgtgcgtaa tgtgattctt tctggctatt ttgattcgat
    28021 tggcatttat caggccgttt cggatcgcca gcaaaaactg gtgcagcagt ggctggatat
    28081 tctcggcatt gataaacgca cggctgacgc tcctttccat agtctttcct ggggacagca
    28141 gcgtctggcg ctgattgtcc gcgcactggt gaaacatccg acgttgctta ttctcgatga
    28201 accactacag gggcttgatc cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct
    28261 gattagcgaa ggtgaaacgc aattgttgtt tgtttcgcac cacgctgaag atgcgcctgc
    28321 ctgtattacc catcgtcttg agttcgtgcc ggacggtgga ctctatcgct atgtgctgac
    28381 aaaaataaat tgagtcggta atgctgaccg tgccggaggc ggcgtaaacg tgttatccgg
    28441 ccaacggttt gacgcatcca ggcatgaaac cgcgtctttt ttttcagata aaaagcgcaa
    28501 tcattcataa agccgctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattcc
    28561 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
    28621 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
    28681 gcggttacat tggaagtcat acctgtgtgc aattactgca aaacggtcat gatgtcatca
    28741 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
    28801 gcaaacatcc aacgtttgtt gaaggcgata tccgtaacga agcgttgatg accgagatcc
    28861 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gttggcgaat
    28921 cggtacaaaa accgcttgaa tattacgaca acaatgtcaa cggtactctg cgcctgatta
    28981 gcgccatgcg cgccgctaac gtcaaaaact ttatctttag ctcctccgcc accgtttatg
    29041 gcgatcagcc caaaattcca tatgttgaaa gcttcccgac cggcacaccg caaagtcctt
    29101 acggcaaaag caagctgatg gtggaacaga tccttaccga tctgcaaaaa gcccaaccgg
    29161 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggtgata
    29221 tgggcgaaga tccgcaaggc attccgaata acctgatgcc gtacattgcc caggttgctg
    29281 taggccgtcg cgactcactg gcgatttttg gtaacgatta tccgaccgaa gacggcactg
    29341 gcgtgcgcga ttacatccac gtaatggatc tggcagatgg tcacgtcgtg gcgatggaaa
    29401 aactggcgaa caagccaggc gtacacatct acaacctcgg cgcaggcgta ggcagcagcg
    29461 tgctggacgt agttaatgcc ttcagtaaag cctgcggcaa accggttaac tatcattttg
    29521 caccacgtcg cgcgggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
    29581 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
    29641 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
    29701 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
    29761 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
    29821 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
    29881 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
    29941 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
    30001 gggtgatctg cttttcaccg gatcacagta aaacgctgcc agaactgagc gttgcggcat
    30061 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga actggggaaa acatacccgt
    30121 gggtgcaggt ctttgaaaac aaaggcgcgg cgatgggctg ctctaacccg catccgcacg
    30181 gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgtctgcaaa
    30241 aagaatattt cgccgggcag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
    30301 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgtcgtg ccttactggg
    30361 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttacgg atcaccgatt
    30421 tgaccgacgc ccagcgcagc gatttggctc tggcgttgaa aaagctgacc agtcgttatg
    30481 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgcg ccatttaatg
    30541 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ttgcgctctg
    30601 ccaccgtacg taaatttatg gttggttatg aaatgctggc agaaacccag cgagacctga
    30661 ccgcagaaca ggccgcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
    30721 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
    30781 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
    30841 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
    30901 cgccacgcga tgaccgtaaa gttcgcgtaa tggcagccga ttatgaaaat cagctcgacg
    30961 agttttccct cgatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
    31021 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
    31081 tcagcggcaa tgtgccgcag ggtgcagggt taagttcttc cgcttcactg gaagtcgcgg
    31141 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
    31201 acggtcagga agcagaaaac cagtttgtag gctgtaactg tggaatcatg gatcaactga
    31261 tttccgcgct cggcaagaaa gatcatgcct tgctgatcga ttgccgctca ctggggacca
    31321 aagctgtttc catgccgaaa ggcgtggctg tcgtcatcat caacagtaac ttcaaacgta
    31381 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
    31441 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgaac
    31501 tggacccgat cgtcgcgaaa cgcgtgcgtc acatcctgac tgaaaacgcc cgcaccgttg
    31561 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt
    31621 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtacc gcaaattgac actctggtag
    31681 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    31741 gtggctgtat cgtcgcgttg atcccggaag aactggtgcc tgccgtacag caagctgtcg
    31801 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    31861 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    31921 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    31981 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgcttggctg
    32041 cgccagcccg gaatgctatc aggatcaggc cgcatttctg ggggcctcta ttggtcgtta
    32101 tgccaaccgc atcgccaata gccgttatac ctttgacggt gaaaccgtga cgctttcacc
    32161 aagtcagggc gtcaaccaat tgcatggcgg cccggaaggg ttcgacaaac gtcgctggca
    32221 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    32281 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    32341 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    32401 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cataagttgc agattctggc
    32461 ggaagaatat ctgccggttg atgaaggcgg cattccgcac gacggcctga aatctgtcgc
    32521 cggaacctct tttgatttcc gcaacgccaa aatcatcgcc agtgagtttc ttgccgacga
    32581 cgatcagcgc aaagtgaaag gttacgatca cgcgttcttg ttacaggcca aaggcgatgg
    32641 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    32701 caccgctccg gctctgcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    32761 aaccgaacct tacgctgact ggcaagggct ggctctggaa agcgaatttc tgccggacag
    32821 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    32881 cagcctgacg gaatatcagt ttattgctca gtaatgttaa gccctcgtca tgagggcttt
    32941 atcctcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    33001 caaccttaca ctgcgacact attttcgcta tggttatgcg taagca
//
LOCUS       DAJOTB010000039        33007 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJOTB010000039 DAJOTB010000000
VERSION     DAJOTB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.039"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            16..1811
                     /locus_tag="OHN28_001820"
                     /pseudo
     CDS             16..1811
                     /locus_tag="OHN28_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
     gene            1829..2497
                     /locus_tag="OHN28_001821"
     CDS             1829..2497
                     /locus_tag="OHN28_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HCQ2297225.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKEHWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLKKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2512..3183
                     /gene="dsbI"
                     /locus_tag="OHN28_001822"
     CDS             2512..3183
                     /gene="dsbI"
                     /locus_tag="OHN28_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HCQ2297226.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3229..4017)
                     /gene="ygiD"
                     /locus_tag="OHN28_001823"
     CDS             complement(3229..4017)
                     /gene="ygiD"
                     /locus_tag="OHN28_001823"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCQ2297227.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVA
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4160..4933
                     /gene="zupT"
                     /locus_tag="OHN28_001824"
     CDS             4160..4933
                     /gene="zupT"
                     /locus_tag="OHN28_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCQ2297228.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(4991..5161)
                     /locus_tag="OHN28_001825"
     CDS             complement(4991..5161)
                     /locus_tag="OHN28_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297229.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTKEETAEKVE"
     gene            complement(5423..6076)
                     /gene="ribB"
                     /locus_tag="OHN28_001826"
     CDS             complement(5423..6076)
                     /gene="ribB"
                     /locus_tag="OHN28_001826"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCQ2297230.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6180..6328)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6450..6749
                     /gene="ubiK"
                     /locus_tag="OHN28_001827"
     CDS             6450..6749
                     /gene="ubiK"
                     /locus_tag="OHN28_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCQ2297231.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARYNPVAELQSPPAIPPVDKAE"
     gene            complement(6784..6984)
                     /gene="glgS"
                     /locus_tag="OHN28_001828"
     CDS             complement(6784..6984)
                     /gene="glgS"
                     /locus_tag="OHN28_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCQ2297232.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7254..7883
                     /locus_tag="OHN28_001829"
     CDS             7254..7883
                     /locus_tag="OHN28_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCQ2297233.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWSQIL"
     gene            7910..9571
                     /locus_tag="OHN28_001830"
     CDS             7910..9571
                     /locus_tag="OHN28_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCQ2297234.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9811..9870
                     /locus_tag="OHN28_001831"
     CDS             9811..9870
                     /locus_tag="OHN28_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2297235.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(9990..11423)
                     /gene="hldE"
                     /locus_tag="OHN28_001832"
     CDS             complement(9990..11423)
                     /gene="hldE"
                     /locus_tag="OHN28_001832"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCQ2297236.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11471..14311)
                     /gene="glnE"
                     /locus_tag="OHN28_001833"
     CDS             complement(11471..14311)
                     /gene="glnE"
                     /locus_tag="OHN28_001833"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCQ2297237.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14334..15635)
                     /gene="ygiF"
                     /locus_tag="OHN28_001834"
     CDS             complement(14334..15635)
                     /gene="ygiF"
                     /locus_tag="OHN28_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCQ2297238.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSRIEIAFDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            complement(15705..16157)
                     /gene="tnpA"
                     /locus_tag="OHN28_001835"
     CDS             complement(15705..16157)
                     /gene="tnpA"
                     /locus_tag="OHN28_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc41 family
                     transposase"
                     /protein_id="HCQ2297239.1"
                     /translation="MWLFRMYNISTFFWEGQYVCEINRSRYSAYLLHVHVVFMTKYRR
                     PVFGELHHANMRQYVAEVCADFGAELKECDGEADHVHMLIEYPPLVQLTKLVNSLKSV
                     TSRRLRNDFIDLRAAYSKPVLWSRSYFIGSCGGAPLEVVKKYIQNQRG"
     gene            16170..17375
                     /locus_tag="OHN28_001836"
     CDS             16170..17375
                     /locus_tag="OHN28_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2297240.1"
                     /translation="MLIQKAYKYRLYPTDQQAQRLRQLCGCARFVWNYALNETLSIHD
                     AGGKIPSAFDLNKRLTGWKKLPELAFLSEGYTDNLQQKLKDLRSAWDRCFDKSLTAEK
                     PVFKKKTKGCDSIRFVNFSKYCGLDYGRVKLPSGLGWVKFRQSRKIEGVIKNCTISQH
                     AGHWYVSFQVELAVTDPIHASTSAIGLDAGITKLATLSDGTVFEPVNSLKKNQDKLAR
                     LQRRLARMVKFSANWKKQKAKISRFHSHIANIRRDYLHKTTTTISKNHAMIVIEDLKV
                     SNMSKSAAGTVDQPGRNVAAKSGLNRAILDQGWAEMRRQLEYKQAWRGGDVLAINPAY
                     TSQKCACCGHTSKNNRRTQASFICTACGYTANADVNGARNILTAGFEVMAAGQILPSV
                     RKGRARKAA"
     gene            17718..18338
                     /locus_tag="OHN28_001837"
     CDS             17718..18338
                     /locus_tag="OHN28_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCQ2297241.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            18402..19640
                     /gene="cca"
                     /locus_tag="OHN28_001838"
     CDS             18402..19640
                     /gene="cca"
                     /locus_tag="OHN28_001838"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCQ2297242.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEILDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(19720..20541)
                     /gene="bacA"
                     /locus_tag="OHN28_001839"
     CDS             complement(19720..20541)
                     /gene="bacA"
                     /locus_tag="OHN28_001839"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2297243.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(20632..21000)
                     /gene="folB"
                     /locus_tag="OHN28_001840"
     CDS             complement(20632..21000)
                     /gene="folB"
                     /locus_tag="OHN28_001840"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCQ2297244.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            21105..21722
                     /gene="plsY"
                     /locus_tag="OHN28_001841"
     CDS             21105..21722
                     /gene="plsY"
                     /locus_tag="OHN28_001841"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCQ2297245.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(21735..22667)
                     /gene="ttdR"
                     /locus_tag="OHN28_001842"
                     /gene_synonym="dan"
     CDS             complement(21735..22667)
                     /gene="ttdR"
                     /locus_tag="OHN28_001842"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCQ2297246.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            22874..23785
                     /gene="ttdA"
                     /locus_tag="OHN28_001843"
     CDS             22874..23785
                     /gene="ttdA"
                     /locus_tag="OHN28_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCQ2297247.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            23782..24387
                     /gene="ttdB"
                     /locus_tag="OHN28_001844"
     CDS             23782..24387
                     /gene="ttdB"
                     /locus_tag="OHN28_001844"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCQ2297248.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            24435..25898
                     /gene="ttdT"
                     /locus_tag="OHN28_001845"
     CDS             24435..25898
                     /gene="ttdT"
                     /locus_tag="OHN28_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCQ2297249.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(25941..26954)
                     /gene="tsaD"
                     /locus_tag="OHN28_001846"
     CDS             complement(25941..26954)
                     /gene="tsaD"
                     /locus_tag="OHN28_001846"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCQ2297250.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            27192..27407
                     /gene="rpsU"
                     /locus_tag="OHN28_001847"
     CDS             27192..27407
                     /gene="rpsU"
                     /locus_tag="OHN28_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCQ2297251.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            27518..29263
                     /gene="dnaG"
                     /locus_tag="OHN28_001848"
     CDS             27518..29263
                     /gene="dnaG"
                     /locus_tag="OHN28_001848"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCQ2297252.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            29458..31299
                     /gene="rpoD"
                     /locus_tag="OHN28_001849"
     CDS             29458..31299
                     /gene="rpoD"
                     /locus_tag="OHN28_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCQ2297253.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(31377..31883)
                     /gene="mug"
                     /locus_tag="OHN28_001850"
     CDS             complement(31377..31883)
                     /gene="mug"
                     /locus_tag="OHN28_001850"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCQ2297254.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            32008..32083
                     /locus_tag="OHN28_001851"
     tRNA            32008..32083
                     /locus_tag="OHN28_001851"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32041..32043,aa:Ile,seq:cat)
     gene            complement(32137..32901)
                     /gene="nfeF"
                     /locus_tag="OHN28_001852"
     CDS             complement(32137..32901)
                     /gene="nfeF"
                     /locus_tag="OHN28_001852"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCQ2297255.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
BASE COUNT         8225 a         8790 c         8246 g         7746 t
ORIGIN      
        1 taaggagata tatccatgtt tgataaatat agaaaaacac tcgtagccgg aactgtggcg
       61 ataaccctgg gtttgtcagc atcgggggtg atggctgcgg gttttaaacc agcgccacct
      121 gccgggcaac tgggtgcggt cattgtcgat ccctacggca atgcaccact gaccgctttg
      181 gttgacttag atagccatgt tatttctgac gtcaaagtta ccgtccatgg gaagggcgaa
      241 aaaggcgtag aaatcagcta tcccgtgggt caggaatcac taaaaactta cgatggtgta
      301 ccgattttgg tctttatcag aaatttgcta acaaagtgac cgttgagtgg aaagaaaacg
      361 gcaaggtcat gaaagatgat tatgtggtgc acacttcggc catcgtcaat aattacatgg
      421 ataaccgctc tatctccgat ttacaacaga ccaaagttat taaagtcgca ccgggttttg
      481 aagatcgcct ctatctggtt aatacccaca cctttaccgc ccaaggttcc gatctccact
      541 ggcatggtga gaaagataaa aatgccggta tccttgatgc gggtccggca accggcgcac
      601 tcccttttga tatcgcgccc tttactttca tcgtcgatac cgaaggtgaa taccgctggt
      661 ggctggatca agacactttc tacgatggtc gtgaccgcga tatcaacaag cgtggttatc
      721 tgatgggtat ccgcgaaacg ccacgcggca cctttaccgc tgtacaaggt cagcactggt
      781 acgagttcga catgatggga caggtgctcg aagatcacaa actaccgcgc ggatttgctg
      841 acgccactca tgagtccatt gagacgccaa atggcacggt actgttgcgc gtaggtaaga
      901 gtaactatcg tcgcgatgac ggcgtacacg tcaccaccat tcgtgaccat atcctcgaag
      961 tcgataaatc tggtcgcgtt gtcgatgtat gggatctgac caagatcctc gatccgaaac
     1021 gcgatgcact gctcggcgcg ctggatgcag gtgcagtttg cgttaacgtt gaccttgccc
     1081 atgcaggaca acaggcaaaa ctggaaccag atacaccgtt cggcgacgcg ctgggtgtag
     1141 ggccaggccg caactgggcg cacgtgaact ctatcgccta tgacgcaaaa gatgactcta
     1201 tcatcctctc ctctcgccac cagggtgttg tgaaaattgg tcgtgataag caagtgaaat
     1261 ggatccttgc gccctctaaa ggttgggaaa aaccgctggc cagcaagctg ctgaaaccgg
     1321 ttgatgctaa cggtaagcca attacctgta acgaaaatgg cctgtgcgaa aactcagact
     1381 tcgactttac ctacacccag cataccgcct ggatttccag caaaggaacg ctcaccattt
     1441 ttgataatgg cgatgggcgt catctggaac aacctgcctt accaaccatg aaatactctc
     1501 gctttgtgga atataagatt gatgagaaga aaggcaccgt tcagcaagtg tgggaatacg
     1561 gtaaagaacg tggctacgat ttctatagcc cgattacctc catcattgaa tatcaagccg
     1621 accgtaacac catgtttggc ttcggtggtt ctattcattt gttcgatgtc gggcagccaa
     1681 ccgtcggtaa gttgaacgaa atcgattaca aaaccaaaga agtgaaagtg gaaatcgacg
     1741 tgctgtcaga taaacccaat cagactcact accgtgcgct gttagtccgt ccacaacaga
     1801 ttttcaaata attaagaagg gaataaaaat gtcgaaatta gggatctcat cactatttaa
     1861 aaccatactg cttaccgcag ctttggctgt ttcatttacc gcttctgcat ttaccgaagg
     1921 cacggattac atggttctgg aaaaacctat tccgaatgcc gacaaaacgc tgattaaggt
     1981 atttagctac gcctgcccgt tctgttacaa gtacgacaaa gcggttaccg gtccggtatc
     2041 ggaaaaagtg aaagatatcg ttgctttcac gccgttccat ctggaaacca aaggtgaata
     2101 cggcaaacaa gcaagcgaag tctttgcggt cttgatcaat aaagataaag cagcaggcat
     2161 ttccttattt gatgctaact ctcagtttaa gaaagccaag tttgcctact acgcggctta
     2221 tcacgacaaa aaagaacact ggtctgacgg taaagatccg gctgctttta tcaaaaccgg
     2281 tctggatgcc gcaggtatga gtcaggcgga ttttgaagcg gcactgaaag aaccagcggt
     2341 tcaagaaaca ctgaaaaaat ggaaagcctc ttatgatgtc gccaaaatcc agggggttcc
     2401 agcctatgtc gttaatggta agtacttgat ctacaccaag agcatcaaat ccatcgacgc
     2461 gatggcagac cttatccgtg agttagcaag caaataacag ggaaacaggc tatgggtatt
     2521 aagggaatgt ggaaagatct tcgcacatca ccagttgaca cactggtcag atggcaggag
     2581 caacgcttgt tgtggttgtt aatggccgtt gcgatgggcg cgttaattat cctggcgcac
     2641 tctttcttcc agatctatct ctacatggca ccctgtgagc aatgtgtata cattcgctac
     2701 gccatgtttg tgatggttat tggaggattg gttgcagcga tcaacccaaa gaacatcatc
     2761 ctaaagctaa ttggctgcgt gatggcattt tacggcagca ttttgggact gaagttttcg
     2821 ctcaaactga acgatatcca ccatgcagta cataatccgg atcccgattc actgtttggc
     2881 gtacagggtt gctctaccga tcccactttt ccctttaacc tgccgctggc acagtgggca
     2941 ccaaattggt tcaagcctac cggggattgt ggctatgacg caccaatcgt tcctgatggc
     3001 gtcacactga gcagcacgca acagtggttt gtggaaatgt atcagcaatc tgaaggctgg
     3061 tatctgctac caccatggca ttttatgaat atggctcagg cttgtatgct ggcttttggt
     3121 atgtgtctgg tgctgctggt cattatgagt ggcgcatggg cgcttaaaat catcagaggc
     3181 taactcagta acacccgaag ccagtcgttc tggcttcggg ttcttttatt aacctatctg
     3241 caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg taattggctc
     3301 ctgcccatcc cacgccccta agacatacaa caacggcaga tagtgctctg gcgttgggtt
     3361 cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgttgtt ccaccggccc
     3421 ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg gatacggtga
     3481 actatcaccg tgccacttca ctgtacgcag gttatgtacc acgttgccac tggcgaccaa
     3541 cattatccct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat gccaggcggc
     3601 aggtttgcta ctgtcgatac tcaactgcac catcgggata tcggcgtcag ggtacatctt
     3661 aatcagcacg ccccaggaac cgtggtcaaa gccccaggct tctttatcca gcgccacagg
     3721 aaccggcgct aacagctcaa ccagatgctg tgccagcgca ggtgaacccg gagcaggata
     3781 atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg gcggcatctc
     3841 catcgcggtc actcctgttc cacgggtaaa ccaatgagcc gaaaccacca ctattgcttg
     3901 cgggcgtggc aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat tatcttccag
     3961 cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg aagacatgat
     4021 gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt cggcggagta
     4081 agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc tgaaggtgaa
     4141 gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg cgggggcagc
     4201 cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc
     4261 gttttcgcta ggatttgcgg cggggatcat gttgctcatc tcattaatgg aaatgcttcc
     4321 tgccgcatta gccgccgaag gaatgtcacc tgtgttgggt tatggaatgt ttatcttcgg
     4381 tctgcttggc tattttggtc tggaccgcat gttgccacat gcgcatccgc aggatttaat
     4441 gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc tgctcactct
     4501 cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg cgagcagcaa
     4561 cctggagctg ggatttggca tcgccctggc cgtagcgttg cacaatattc ctgaaggtct
     4621 ggcggttgca ggcccggttt acgctgcaac aggatcgaaa cgtaccgcga ttctgtgggc
     4681 gggcatttcc gggctggcag aaattcttgg tggtgtgctg gcatggttaa tcctcggtag
     4741 catgatttct ccggtagtca tggcggcaat catggcggcg gttgcaggaa ttatggtggc
     4801 gctctccgtt gacgaattaa tgccgctcgc caaagaaatt gaccccaata ataaccccag
     4861 ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc tacaaacggt
     4921 ggggattggt taaaagcaac atcagaagtt gtccagcgca tggacaactt ctgtcagctt
     4981 cactaaagtc ttattctacc ttttcggctg tctcttcttt cgtactgttt aagtatttgt
     5041 tccgcgcttc aagcagggcg tctctgtcct ggcgaaacgc ccgacaatat cgtttcaaat
     5101 gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag gcgataaaca
     5161 tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt cacatatcct
     5221 ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa
     5281 tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat
     5341 cttgcatagc aggtaaaaat aaaaaccgaa gcctgctaca acttcggttt cttgattaag
     5401 gcagtaaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca
     5461 ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca atgcactctg
     5521 gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca gctggtttaa
     5581 agcctgccag cgtcatcaga tcgatagtcg cttcagtatg accgccacgc gtcagtacac
     5641 cacctgcctg cgcgcgaagt gggaaaacgt ggcctgggcg attcagatct gaaggttttg
     5701 cgccatcggc aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag
     5761 tcacaccttc agctgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat
     5821 tttctaccat cattggcaga tcgagttgtt tacggcgatc gtcagtaatg cacaggcaaa
     5881 caataccact cccgtggcga atggtcagcg ccatctgctc aacggtcatg gtttctgccg
     5941 ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatc acaccgcgtc
     6001 cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa
     6061 gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca
     6121 gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc
     6181 gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc
     6241 ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg
     6301 ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga ttagcggcag
     6361 caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac
     6421 tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg
     6481 ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga
     6541 aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct cgatctggtg agccgcgaag
     6601 aattcgacgt acagacgcaa gtcctgttac ggactcgtga aaaactggcg ttactggagc
     6661 agcgcatcag cgaactggaa gcgcgctata atccggtagc agaacttcaa tctcctccgg
     6721 ctatcccgcc agttgataaa gcagaataaa gcaccacaaa aacgggccag acggcccgta
     6781 atatcagtgt tctaattcaa gttctctggt ttgcatcatc tgttggcaat accaggcata
     6841 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc
     6901 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gcgagaaaat cgaaattatt
     6961 taaagaatta agactatgat ccataagcac atcctccggt tattgcatac cttttaaggc
     7021 catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg
     7081 cgtaaatata ttgtccccga tcacactttt tagtgaaaaa taaaaaaacc atattaaaag
     7141 actttctctt ccagaacctc tctaacatgt gttattatta acaaagataa atgtaagcaa
     7201 ggaagctatt ccaaagtaaa tattgcgtca ctaaatggac attggagtga catatgattt
     7261 tattcgccga ctataacacc ccttatcttt tcgctatttc ctttgtactg ttgatcggcc
     7321 ttttagaaat ttttgcactt atctgcggtc acatgctctc cggtgctctt gatgcacatc
     7381 ttgaccatta tgattccata accacaggtc atattagcca ggcacttcat tatctcaata
     7441 ttggaagatt accagctctc gtcgtcctct gcttactcgc tggttttttt ggtcttattg
     7501 gcatcctgtt gcaacatgcc tgcgttacgc tctggcaatc accactcccc aacctgttcg
     7561 tggttcctgt cagtttactg tttacaatta ttgcagtgca ttataccggt aaagtcgttg
     7621 caccctggat tccacgcgac catagttcag ctattacaga agaagaatat gttggtagca
     7681 tggcattaat taccggccat caagccactt caggtaaccc ctgcgaagga aaactcaccg
     7741 atcaatttgg ccaaatacat tatttgttac tggagcctga agaaggaaaa actttcacta
     7801 aaggagacaa agtgttaatt atttgccgac tttctgcaac gcgatatttg gcggaaaaca
     7861 atccctggtc ccagatattg tgatttatta atcaaaagga aatttaataa tggatgatat
     7921 cgttaattct gtgccctcct ggatgtttac cgcgattatc gccgtatgca ttctgtttat
     7981 tattggaatt attttcgcca ggctctatcg tcgcgcttcg gcagagcaag catttgttcg
     8041 tactggttta agtgggcaaa aagtggtgat gagcggtggc gcaatcgtga tgccgatctt
     8101 tcatgaaata attcccatca atatgaatac cctgaagctg gaagtcagcc gttctactat
     8161 tgatagcctg attacgaaag atcgtatgcg tgttgatgtt gtagtggctt tctttgtacg
     8221 ggtaaaaccg tcagtagaag gcatcgccac tgcggcccag acgctggggc aacgcactct
     8281 atcaccagaa gatttacgta tgctggtcga ggataaattc gtcgatgccc tccgtgcaac
     8341 agcggcacaa atgaccatgc atgagttaca ggatacccgc gagaacttcg tgcagggagt
     8401 acaaaatacc gtagctgaag atctgtcgaa aaatggcctg gaactggaaa gcgtttcact
     8461 taccaacttt aaccagacct cgaaagaaca tttcaatccg aacaatgcct ttgacgccga
     8521 aggtttaacc aaactgactc aggagacgga gcgccgtcgt cgcgaacgta acgaagttga
     8581 acaggatgtg gaagtcgcag tgcgtgagaa aaaccgtgat gcactttcgc gcaagttgga
     8641 gattgaacaa caagaagcgt ttatgacgct tgagcaggag cagcaggtta aaacccgtac
     8701 cgctgagcag aatgcgaaaa ttgcggcttt tgaagctgaa cgtcgtcgtg aagcagagca
     8761 gacgcgaatt ctggctgaac gacagattca ggaaacagaa atcgaccgcg aacaggccgt
     8821 ccgctcaaga aaggttgagg ccgaacgtga agttcgcatt aaagagatcg aacagcagca
     8881 ggtcaccgaa atcgctaacc agacgaaatc gatcgctatt gccgccaaat cggaacaaca
     8941 gtcccaggca gaagcgcgtg ctaaccttgc actcgcagaa gcagtaagcg cccaacaaaa
     9001 cgtagaaacc actcgccaga ctgccgaagc cgatcgtgct aaacaagtgg cgttgatcgc
     9061 cgcagcgcag gatgcagaaa ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa
     9121 agaagccgca gagatgcagg cggcggctat cgttgagtta gccgaagcta cacgtaaaaa
     9181 gggtctggcg gaagcagaag cacaacgtgc gctgaacgat gctatcaacg tactttctga
     9241 tgaacaaacc agccttaaat tcaaactggc attattgcag gcgctgcctg cggtaattga
     9301 gaaatccgtg gaaccaatga agtctatcga cggcatcaaa attattcagg tcgatgggct
     9361 gaatcgtggt ggcgctacgg gtgatgcaaa cacgggtaat gtggggggcg gaaacctggc
     9421 agaacaagcg ttatcagccg cactctctta ccgcacacag gcaccgctga ttgactcctt
     9481 gctcaatgaa attggcgttt caggcggctc actggcggca ttgacttcat ccttaagctc
     9541 aacaactccc gtcgaagaaa aagcagagta agccctcttc tatgaataat ccctggccca
     9601 aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgat caggctgttg
     9661 cagcattttg cgtgactgct cggggaaggt aaaactgcct ctgggaaagc gttgcgcggg
     9721 gtccggcgct catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc
     9781 gaggaagggg ccttgtataa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg
     9841 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
     9901 tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaataggc ccgcctgcta
     9961 caaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg
    10021 atgatgttgg tcgtcgagca accgtcttca aagttgagca ccagcacttc gccaccgttg
    10081 gcccagactt ctttactccc ggcaatctct tctggtttat agtcgccgcc tttcaccagt
    10141 agatctggca agatcccggc gatcaagcgc tgcggcgtgt cctcttcaaa cgacaccacc
    10201 cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg
    10261 cgggaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcaaacgg
    10321 tcacccagtt tacgggcatt tgccagataa gagacgtgcc cagcgtgcag gatgtcaaag
    10381 acaccgttgg tcatcaccac tttttcaccg cgtttacgcg ctgccgctac agcaagcttc
    10441 agttcctctt cggtcatcac accaaagccg gtatctgcac gtccacgtac tgcgttttcc
    10501 agctcgatcg gcgaaaccgt ggatgtcccc agtttgccga ccaccacacc agccgccgca
    10561 ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg
    10621 ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata
    10681 tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac
    10741 agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg
    10801 gtcttacatt taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagca
    10861 ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcactggaac acccgcttta
    10921 cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc
    10981 accagcgcgc caatcgaact cagtgcctga ttaatccgct cgtgcagcgg ctgcggatca
    11041 accccttcga aaccttcttc gaaatccaga cggatcagtt gttggttgcg ggaaagtacc
    11101 cgtaatttgg tgatggtcgg atgcgtcggt acagaaacaa agtcgcattt gacgttgacg
    11161 tcggccagag atttactgag cgcgcgcgcc gcatcgtcaa tgcccgtcaa cccgaccagg
    11221 cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt
    11281 tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat acgactggtg
    11341 ggaccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc tgcacgttca
    11401 aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata
    11461 ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc agttcacgct
    11521 ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa
    11581 gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa
    11641 tgtcgttttg cgccagtagt tccagaatgc gcacgttgtc tgaccagcgc gttaacttcg
    11701 gtttttcgtg agcgtagcgc aacaccagat attgagtaat aaattcgata tcggtaattc
    11761 cgccttcatc ggctttgata tcaaagcgat cgcgatgttt attgccgaga tgggcacgca
    11821 ttttctcgcg catttcccgc acttctgttt gcagggtttt gccttcgcgc ggcagcgtca
    11881 taatctcccg gcgtactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactacac
    11941 gcgcacgcac cagcgcctga tgctcccacg tccaggcctc gtttttctga taatcagcaa
    12001 atgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcagt cgtgcatcca
    12061 cttcatacaa aatgccggaa gaggttcgcg tactgaacag atgcataatg cgttgcgcca
    12121 gacgcagata aaactgtcgc ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg
    12181 ggcaatcatg gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccaccca
    12241 gcttgccgta gccgaccacc gcaaaaccac gcccttcgcg ttcgttcagg tgatttggct
    12301 taccgtagcg ggcaaccatt tgaacccacg cctgctgaac gacggcatca atcatggctt
    12361 ctgccagcca ggttaagtga tcgctcactt tcattaccgg tagcgtaccg gcgatatccg
    12421 ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt
    12481 cttcgtcatc ttccggcacg cgcagcaaat actggcgcag ttcatcacgg taggcatccg
    12541 tcgccgttgg ttggtagagg gtatttggat cgagcaattc atccagcaat aatggataac
    12601 gcgctagctg gctggcaatc atcggcgatg cagcacacag agaaatcaaa tgcttaagcg
    12661 ccgcagggaa ttcactaagc agctcaagat aagtggtgcg agtaacaatc cccaccagca
    12721 aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca
    12781 gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatagtg cgcttatcca
    12841 gctctttgcg gaaatcagca atcagcatta gcacctgttt gcgatcatcc tcgctaagat
    12901 gcgccagcac tggcgtggtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact
    12961 gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca
    13021 cccggcgcac attggccata tgtgcggtca gcaccccggt caattgcggc cagtcagcaa
    13081 agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt
    13141 gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc
    13201 gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatggcg ctgagcgttg
    13261 gcagtaaagc gcgcgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaacacct
    13321 ggacgataaa ttcaatttcg cgaatgccgc ctgcgccaag tttgatgttg tcggtcaaac
    13381 cgcgtcgacg cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc
    13441 tgaaatcgat gtaacgacgg aaaacaaacg gacgcagcat cgcacgcaac tcgttagcat
    13501 agacgccgtc gctatccccc ataatccgcg ccttgaccat cgcgtaacgc tcccagtcgc
    13561 gcccctgctc ctggtaataa tcttccagcg ccgcaaaact cagcaccagc gggccacttt
    13621 caccaaacgg acgcagccgc atatccacgc gatagacgaa gccatcctgc gttggttgat
    13681 ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc
    13741 ggcgtccacc ctgggtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg
    13801 agaaattcag ttccccgccg cccagcttac ccatgcctaa aatcagcagc ggttgcgctt
    13861 cgccctgcgc attgcacggc gttccccatt cgcggcagca ggcgtcatac agccagtcac
    13921 gcgccgcaac aatcagcgtc tccgccagat ggctgagctg ctgcaatatg ctctcttcag
    13981 taaccagagc cagcgtttgc gcccaggcga tgcgcaccat aatgcgccgc cggaatagcc
    14041 gcagctcgcg cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcagccatg
    14101 cagcgtaatg ctgccattcg tcggcctgcg gcggttggct ttccagttcc gtcagccact
    14161 ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct
    14221 gtgcgctaag tgattcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt
    14281 actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt
    14341 tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt
    14401 tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccagtct
    14461 tgcgcgacga caggatcgta gtaacccgcc agcaacagta ttgagtcaat atcacgcgtc
    14521 aggcgtggca attggtcacg gtagcgatcg cctaacggct ggcagaaaac gcttttcagt
    14581 tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggaatc gctgattttg
    14641 ctctgcgctt tggcatctaa aaacggctgc caggctttgc ttaccagcca ttcggtcaac
    14701 gccagcttcg ccattgccgt ttcggtagag tagaccgccg ttacggcaga aaccgcagaa
    14761 gcaatggtcg cctcgcattg agtcagcaga tcacgtaagt gagtgctcgc tttacgcggc
    14821 acgataccgc cgaacagcat cagtgtatga cggaccaggc caatggctgc cagcacctgc
    14881 tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca ttgcgctaac
    14941 gccagctcga gcgccgcttc cagcccctgt tccacatcag cttttgccgc aacatgcaaa
    15001 atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgct
    15061 gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc
    15121 agcaccgcac gcatgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca
    15181 aattcacccg ctttcacctc accctgatcg aaagcgattt caattcggct gccatcgacc
    15241 tctactagcc atttttcgcg ataaaaatcg gtgctaaaca gcggctgtac gcgggaagcg
    15301 agatcggcag gcaattcgcc gttcggccag acttccgtcg gtaactgcgc caggtcgagc
    15361 gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt
    15421 cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg caagcccatg
    15481 tcgtgcccac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacggga
    15541 tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga
    15601 ttaacaataa actttaattc gatttcctga gccatccttc ctccactggg tactcccccc
    15661 aacgtaggcg ggaggggaat ttgggctgtt aagcccgcag tttatcagcc acgctgattc
    15721 tgaatatact ttttcacaac ttccagcggt gctccaccac atgaaccgat gaaataagag
    15781 cgtgaccaga gtacgggctt tgaatacgcg gcgcgcaaat caataaaatc attccgcagc
    15841 cgcctactgg taacggactt aaggctgttt accagctttg tgagttgcac caacggtggg
    15901 tattcaatca gcatgtgaac atggtctgcc tcgccgtcac attcctttaa ctcggcgcca
    15961 aagtccgcgc atacctccgc tacatactga cgcatgtttg catgatgcaa ttcaccaaaa
    16021 actggacggc ggtattttgt cataaaaaca acgtgaacat gcaacaggta agctgaatag
    16081 cgtgaacgat tgatttcgca cacgtattga ccctcccaaa aaaatgtgga tatattgtac
    16141 attctaaaca accacatact gaatgcaaaa tgttaatcca gaaagcatat aaatacaggc
    16201 tctaccccac tgaccagcag gcgcaaaggt tgcgtcagtt gtgcggttgc gctcgctttg
    16261 tctggaacta tgcactgaat gaaacactga gcattcatga cgcgggcgga aaaattccgt
    16321 cagctttcga cctgaataaa cgacttacgg gctggaaaaa actccctgag ctggcctttc
    16381 tttctgaggg ttacactgac aacctccagc agaaactgaa agacctgcgg agcgcatggg
    16441 atcgctgttt tgataaatcc ctgacggctg aaaagcccgt tttcaaaaag aaaaccaaag
    16501 gctgcgactc tatccggttc gtcaatttca gcaaatattg tgggctggat tatggccgtg
    16561 tgaaactccc gtcaggactg ggctgggtta aatttcgtca gtcccggaag attgaaggcg
    16621 tcattaaaaa ttgcaccatc agccagcatg ccggacactg gtatgtgtct tttcaggtcg
    16681 aattagctgt taccgatccg attcacgcat cgaccagcgc aataggcctg gatgcaggta
    16741 taacgaagct ggcaacactt tcagacggca cggtctttga accggtaaac agcttaaaaa
    16801 aaaatcagga taagctggct cgcttacagc gccgtctggc ccggatggtt aaattctccg
    16861 ccaactggaa aaagcagaaa gcgaagattt cccgtttcca ctctcatatc gcaaacatcc
    16921 gccgcgatta ccttcataaa accacgacga cgatcagcaa aaaccacgcg atgatagtca
    16981 ttgaggattt aaaggtttcg aacatgtcaa aatcagcagc gggtacggta gaccagccgg
    17041 ggcgcaacgt cgcggcaaaa tcaggtctta accgggcgat actggatcag ggctgggcag
    17101 aaatgcgccg ccagctcgaa tataagcagg catggcgcgg cggtgatgta ctggcgatta
    17161 atccggcgta caccagtcag aaatgcgcat gttgtgggca tacatcgaaa aataaccgca
    17221 ggacgcaggc gagctttatc tgtaccgcgt gcggctatac ggcaaacgcc gatgtaaacg
    17281 gcgctcgcaa tatattaaca gcaggttttg aagtcatggc ggcaggacaa atattgccgt
    17341 cagtccgtaa aggacgcgcc cgtaaggcgg cgtaagccgt cgagcgagga accggggaat
    17401 gcggcaacgt attgctaccc tgacaaggga atccccctcc tgagtcgctt tgcgcgaagg
    17461 tgggggagga tgtcaaggcc tttacttatc ggttatgtca cataagtgac gatgaacggc
    17521 gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg ccatgcaacg
    17581 ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag aggattccga
    17641 ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc actttccatg
    17701 acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta ctcgcactta
    17761 gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct
    17821 gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac gccggcgagg
    17881 aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa gacagctctg
    17941 gtcgtactgc ctggatcccg ttgaaacaac ttagcacgga gccaagcctg cgttcccgtg
    18001 tgccagatct ggaaaatcag gtcaaaacgc tgaccgataa actcaccaat atcgataaca
    18061 cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagtgac agcgtgatca
    18121 acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg cagaaaaagg
    18181 tcgatgccgc cagcgtacag ctggatgaca aacagcgcac catcatcatg cagtggttta
    18241 tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg ccgcacctga
    18301 tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc gttgcactgt
    18361 cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt tatctggtcg
    18421 gtggtgctgt tcgggatgca ttgttagggc taccggtcaa agacagagat tgggtggtgg
    18481 tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc cgcgattttc
    18541 ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc gaacggaaat
    18601 ccggttccgg ttacaccggt tttacctgct atgccgcacc ggatgtcacg ctggaagatg
    18661 atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac ggcgagatta
    18721 tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg
    18781 cttttggcga agatccgtta cgcgtgttgc gcgtagcgcg ttttgctgcg cgttatgccc
    18841 acctcagttt ccgtattgcc gatgaaactc tggcgctgat gcgcgagatg acccatgcgg
    18901 gtgaactgga acacctgacg cctgaacggg tatggaaaga gacggaaaac gccctcacca
    18961 cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg cggcgcactg cgcgttttat
    19021 tcccggaaat tgacgccctg tttggcgttc cggcccctgc caggtggcat ccggaaatcg
    19081 acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt ccgcaggtcg
    19141 atgtccgttt cgcaacgtta tgccacgatc tcggtaaagg actgacgccg ccagaacttt
    19201 ggccacgtca tcatggtcat ggcccggcgg gtgtgaagtt agtggaacaa ctatgccagc
    19261 gtctgcgcgt gccaaatgaa attctcgatt tagccagact ggtggctgag tttcacgatc
    19321 tcatccacac ctttccaatg ctgaacccga aaaccattgt taaattgttt gattccattg
    19381 acgcctggcg taaaccacag cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc
    19441 gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgctggttg cgcgaagcct
    19501 gggaagtggc gcagtcagtg ccgacaaaag ctgtcgttga agcaggattt aaaggtgtgg
    19561 agattcgtga ggagctgacc cgacggcgga ttgcggcggt agccagctgg aaggaacaac
    19621 gttgcccaaa gcctgattaa ctttttaaat tcagtatatt gcagaaatcc cttaggtcgg
    19681 ataagacgca caagcgtcat atccgacact atatctcaat caaaagaaca caacgtacac
    19741 cgcagccgcc acaataaagc gataaatggc gaacgggata aacgaaatgc gcttaatcaa
    19801 ttgcaggaag gttttaatcg ctatcagcgc caccacaaaa gcggtgataa acccaacggc
    19861 aaacatcggg atatcgccgg tagtcaggaa gccccagctt ttgtagagat cgagcgccgt
    19921 tgcgcccatc atcatcggca ccgccagcag gaacgagaac tcggaagcag cgtaacggct
    19981 cacccccatc agcatcccac ctgaaatggt cgccccggaa cgggagaaac ccggccacag
    20041 cgccagacac tggaaacagc caatcataaa cgcctgacga taggtcatat catcaagacc
    20101 cggcgcacgc ggctctttcg gcttcaggca ttcggcggca atcagcaaca gaccgccaac
    20161 gaccagtgca tacatgacat ttatcgggtt aaacaatgat ttaatcgtgt cgtggaacaa
    20221 cagccccagt accaccgccg gaatcatccc cagcaagatg tggatcagcg ttaaacgacc
    20281 tttgctttct ccttcgtgct gcaacgggcg accaaagtgg atgccaatca ggccaaacag
    20341 acgccgccag aacatcacta ctaccgccag aattgatcct aactggatca cgacttcaaa
    20401 ggttttcgcc gtgtcgccct caaaccccaa caagtgaccg acaataatca tatggcccgt
    20461 gctggatacc ggcagaaatt ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag
    20521 cagcgagtgc atatcgctca tcaataaacc cctaaattat aaaaatgtaa cgcttgtccg
    20581 aactactgca gtatgaccag gttataatcg tttggtttaa cagctgtaaa attaattatt
    20641 ttctttcaga ttattgccac gctcaatgat tacgccaaca ttcgccgccc gcgccactgc
    20701 gcctggcttg ctgagtttga tacgcaccca cggcgagttg aagcgtgcta acagcagctc
    20761 cgccacctct tcagccacgc gttccaccag cgcaaaacgc gccccctcga cgtggctgac
    20821 caccgtttct gcaatgtcag cgtaactgag gcaatccgcc acatcatcac ttttcgccgc
    20881 tttacggtta tcccacgcca tttcgatatc gaacactaac ttctgttcga tggtctgttc
    20941 ccagtcgtaa acaccaatag tggtgattac cgaaagttgc tctataaata caatatccat
    21001 cacgtcctgc ctgctttttg gctaaccgga taccacttcc ggcgaaatgt gcgtattatc
    21061 cacagattca tcgttgaaca cgaattttca aaacggaaca gcttatgagt gcaatcgcgc
    21121 ctggaatgat cctcatcgcg tacctctgcg gctccatttc cagtgccatt ctggtttgcc
    21181 gcttgtgtgg gttgcccgat ccgcgaacca gcggctccgg caatccaggc gcaaccaatg
    21241 tgttacgtat cggtggcaag ggagcagccg tagcagtact gattttcgac gttctgaaag
    21301 gaatgttgcc cgtctggggc gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa
    21361 ttgccatcgc cgcctgtctt ggacacatct ggcccgtttt cttcggtttt aaaggaggaa
    21421 aaggcgttgc taccgctttt ggtgccatcg caccaattgg ctgggatctc actggagtaa
    21481 tggcgggaac ctggttactg accgtgctat tgagtggtta ctcgtcgctg ggagcgattg
    21541 tcagtgcact gattgctccg ttttatgtct ggtggtttaa gccacaattc accttcccgg
    21601 tttcgatgct ctcttgcctg atcctgctgc gtcatcatga caacatccag cgtctgtggc
    21661 gtcgccagga gacaaaaatc tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt
    21721 gatttctggt ggatctacat gacctgatag ccttcgtcgg gcttgcccag ccgttgctgg
    21781 caccatgccg ccagaaattc cacacagaca cgtaatttca tgctgcgata gagcggctcc
    21841 cggtaaacag cccagatatt ggcgctttgt gcatactccg gcaatactcg caccagtttg
    21901 ccactctcca gaaacggcaa cacatcccac tcggaacgca gcataatccc tttgccctcc
    21961 agcgcccatt gcaacacaat ctcgccgcta ttggaggaaa gatgtccgct taccttcacc
    22021 gattttttct cctgaccgtt ccccaactcc catattccgt gggtcatatc gcgctctttg
    22081 gtcaccaggc agtcatgacg acttaattcc tgtaaggatt gtggctgcgg atatttttgc
    22141 agatactcag gtgctgcgca taatattctt ttattttttg tcaaaagatg cgcaatataa
    22201 taatcaggaa ttgcgtcatt aatacgaata tccagatcaa tattatcctg cactaaatca
    22261 atttgtcgat cgaacaattc aaaatgcacc tgtaactcag gataattgcg catcagttcg
    22321 gtaatagctg gcgcaatatg gctgcgccca aaaccaaaac tgcaaccaat gcggatcatc
    22381 ccctccgggc gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg
    22441 atttccagcg catgttcgta acaacgctgg ccactttcag tcagcgccac gccgcgagcg
    22501 gagcggttca gcagagttgt tgccagcgta ttttcaagga tttggatccg cttggtgaca
    22561 aatgcaggcg tttgcccaag cgtcgccgct gcagcactaa aactcccgct atggacgatt
    22621 tccaccagta cctgcaagtc tttggctaaa ggccagctat ttagcattgt taaatatccg
    22681 cagttttgat aaacgcagtg tatttcgcaa aacattacca ttccctgctc tggcgcaata
    22741 ttcgttctta ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa
    22801 atataaaacc acatttttag tggtagtttg tggcggtgaa tttttccaga caaatacata
    22861 aactggagtt gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacgga
    22921 gattgtcgct aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa
    22981 actaaaacag ctaaaggatg ccgaaacatc gtcgatggga aaaatcatct accacacgat
    23041 gttcgataac atgcaaaaag cgatcgacct gaatcgtcct gcctgtcagg acaccggcga
    23101 aatcatgttt tttgttaagg tcggttcccg tttcccactg cttggcgagc tgcaaagcat
    23161 actcaaacaa gccgtggaag aggcaaccgt caaagcgcca ctgcgtcaca atgcggtaga
    23221 aatttttgac gaagtaaaca ccggcaaaaa taccggcagc ggcgtaccgt gggtcacctg
    23281 ggatatcgtc cccgacggtg acgatgcgga aatcgaagtt tacatggcag gcggcggctg
    23341 cacgctacct ggccgatcga aagtgttaat gccgtcagaa ggctacgaag gcgtagtgaa
    23401 attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc tgtccgccgg tactggtggg
    23461 cgttggcatc gctacctcgg tggaaaccgc cgcagtgctc tcgcgaaaag ccattttgcg
    23521 cccgattggc agccgccacc ccaatccgaa agcggcagag ctggagctac gcctggaaga
    23581 aggactcaac cgtttgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg
    23641 cgtgcatatc gaatctgccg ctcgccatcc gtcaaccatc ggcgttgctg tttctaccgg
    23701 ctgctgggcg catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct
    23761 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
    23821 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt
    23881 gacgtttgtc accgtcgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc
    23941 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggta
    24001 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag
    24061 accggcgtga aactggtggt cggcaaaggc ggaatggggc cgctgaccga agaaggctgc
    24121 cagaaattca aggcgctgca tgtgattttc ccggctggct gtgcggtact ggcggcaact
    24181 caagtggaag agattgaaga agtgcactgg acagaactcg ggatgccaga gtcgctgtgg
    24241 gtctgccggg tcaaagagtt tggcccgctg attgtctcta tagataccca cggcaacaac
    24301 ctgatagccg aaaataaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
    24361 tgcgagcacg tccactacat caaataaccc tcacggagag gctcgcccct ctcctttttc
    24421 gcaggcataa cacgatgaaa ccttccactg aatggtggcg ataccttgcg ccgctggcgg
    24481 tcatcgccat tattgctcta cttccgcttc ccgcagggct ggagagtcat acctggctct
    24541 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
    24601 tggtggcgat ggtgggtata tccatcatcg ccattctttc gccgtggctg ctgtttagcc
    24661 cggagcagct cgcccagcca ggttttaagt tcaccgccaa atccctctcc tgggcggttt
    24721 ctggtttttc taattcagtt atctggctta ttttcgccgc ctttatgttt ggtacgggct
    24781 atgaaaaaac cgggcttgga cgtcgcatcg cgctgattct ggtgaaaaaa atggggcatc
    24841 gcacgctgtt tctcggctat gcagtgatgt tctccgagct gatactcgcg cctgtaacac
    24901 cgtccaactc ggcgcgtggt gcggggatta tctatcccat catccgtaac ctgccaccgc
    24961 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
    25021 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gctccaaatt
    25081 tgctgttaat tggactgatg aaaagcgcat ctcacgccat gctaagctgg ggcgactggt
    25141 tcctcgggat gttgccgctc agcattttac tggttttgct ggttccctgg ctggcttacg
    25201 tgctgtaccc gccggtactg aagtctggcg atcaggtgcc gcgctgggca gagacggaac
    25261 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
    25321 cactggtgtt gtggattttt ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg
    25381 tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag
    25441 cggcgtggaa cgttttcttc tggctggcct cgcttatcac cctcgctacc ggactcaaca
    25501 acaccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagc ggttattcgc
    25561 caacgatagt gatggtggcg ttgattgtgg tcttttatct gctgcgctac ttttttgcca
    25621 gcgccacagc gtatacctcc gctctcgcac cgatgatgat tgccgccgcg ctggcgatgc
    25681 cggaaatccc gctgccggtg ttctgcctga tggttggtgc ggcaattggt ctggggagca
    25741 ttcttacacc atacgccacc ggacccagtc cgatttacta cggcagtggt tatctgccaa
    25801 cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgta ttgctggtga
    25861 ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg
    25921 ctctccggca cccaatagtt ttacgcggcc ggtaactctg ccagtggcca gcgcggacgc
    25981 acactcacgc cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg
    26041 atcatcgcac cgttatccgt acaaaattcc ggacgcgcgt agaacacttc gccacggcgt
    26101 tttttcatca tttccgccag tttcgcccgc aacgtgcggt tagcactcac gccgcctgcc
    26161 atgaccagtc gcttaaagcc cgtctgatcc agtgctcgct tacacttaat catcaacgta
    26221 tcgaccactg catcttcaaa ggcgcgggcg atatcggcac gcgtctgatc gtcagtgccg
    26281 ttgtcacgta tggtatttgc cgcgaaggtt ttcagaccag aaaagctgaa atccagcccc
    26341 ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt
    26401 ttagacagta acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac
    26461 gcttcaccag cagcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc
    26521 acgctgatta actgcgtatg accgccggaa accagcagcg cgacaaacgg aaattccggt
    26581 gggttatctt ccagcatcgg cgctaacaga tgtccttcca tatgatgtac cgggattgcc
    26641 ggaacgtccc aggcaaacgc cagagaacgc cccacggtcg cgccaaccag cagtgcgccg
    26701 actaatcctg ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac
    26761 tcctttagcg cctcctggat caacggtacg gttttacgca cgtgatcgcg ggaggccagt
    26821 tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct
    26881 aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata
    26941 cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc
    27001 gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta
    27061 taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa
    27121 ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag
    27181 gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc
    27241 gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt
    27301 tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg
    27361 cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg
    27421 ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg
    27481 cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt
    27541 attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa
    27601 gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc
    27661 gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg
    27721 caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga
    27781 gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat
    27841 cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca
    27901 acaatcttta caacaacctg ttgccatgtc tgcgcgccag tatctggaaa aacgcggatt
    27961 aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt
    28021 cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg cgggcatgtt
    28081 ggtcactaac gatcaggggc gcagttacga tcgtttccgc gagcgggtga tgttccccat
    28141 tcgtgataaa cgcggtcggg tgattggttt tggcggacgc gtgctgggca acgatacccc
    28201 caaatacctg aactcgccgg aaacggacat tttccataaa ggccgccagc tttacggtct
    28261 ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat
    28321 ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc
    28381 aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta
    28441 tgacggcgac cgtgcaggcc gcgatgccgc ctggcgagcg ctggaaacgg cgttgcctta
    28501 catgacagac ggtcgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac
    28561 gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact
    28621 ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg
    28681 cgcacgtttg agtacgctgg cactgccact gatatcgcaa gtgccgggcg aaacgctgcg
    28741 aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga
    28801 acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg
    28861 cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt
    28921 cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact
    28981 ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg
    29041 tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa
    29101 gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga
    29161 actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg
    29221 cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc
    29281 cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt
    29341 acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa
    29401 ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg
    29461 gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat
    29521 ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc
    29581 gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat
    29641 gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg
    29701 caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac
    29761 atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag
    29821 cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc
    29881 acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg
    29941 atcaccggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc
    30001 ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga agaagatggt
    30061 gacgacgata ccgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt
    30121 gcggaactgc gcgctcagta cattgtaacg cgtgacacca tcaaagcgaa aggccgcagt
    30181 cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg
    30241 gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt
    30301 acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac
    30361 ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg
    30421 atgaacaagc cgtggtcgga aaaactgcat gatgtctctg aagaagtgca tcgcgccctg
    30481 cagaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc
    30541 aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa
    30601 gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc
    30661 cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa attcgaatac
    30721 cgccgtggtt acaagttctc cacctacgct acctggtgga tccgtcaggc gatcacccgc
    30781 tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac
    30841 aagctcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc aacgccggaa
    30901 gaactggctg aacgcatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc
    30961 aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat
    31021 ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt
    31081 gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg
    31141 cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac
    31201 gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg
    31261 agccgttctg aagtgctgcg tagcttcctg gacgattaat cggtaggccg gatcaggtgt
    31321 tacgccgcac ccggcactag tccctccgca caaacgccac cttttcggtg gcgtttttat
    31381 cgcccacgca ccaccagcgc ctggtccagc tcgcgatacg cttcaaccag tttctccagt
    31441 gaaacgcgac ttaaaccgct gggatttggc agcacccaaa tctgcgtcga accaatggtg
    31501 agcgtttgtt tcccccactg tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg
    31561 cccagaatcg ccaacgcctg cggctgataa tcttcaattt tttcaatcag cttacgcccg
    31621 cctgcgtgta gctcctgctt tgaaacttca ttggcttgca ccgttggacg gtctaccagt
    31681 ttggtgacgc cacaacgata atccagcaga tgctgtgcct cctgcggctt caactgacgg
    31741 tcggtaaacc cggcctgata tatcaccttc cagaagcgat ttgccggatg agcaaaggga
    31801 aaaccagtcc cggcggatga aagaccaggg ttgataccgc aaaacacgac ccgtaaccct
    31861 ggagccaaaa tatcctcaac catatttact cctttctaac atcaccagga aaagtataac
    31921 ggattgaaaa tacattgttt ataaaaacag caggcgcgcg gtaatggctg gattgcgaca
    31981 cggagttact ttataatccg ctaccatggc cccttagctc agtggttaga gcaggcgact
    32041 cataatcgct tggtcgctgg ttcaagtcca gcaggggcca ccagacatag caaaggctga
    32101 cgagaaatcg tcagcctttt tctttttata tatccgttac tttgcgtgcc agtaagccgc
    32161 cgcacgtacc cgctgtgggt catactgttc cgtttcaaag cggcggctta aattcttaac
    32221 gactttgcct tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc
    32281 cagacgcgca tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag
    32341 gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg
    32401 tttaacagca agtttgctca acgtttccag gcggcggcgc agtgcgggca ttccggattc
    32461 atcgcagaca tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc
    32521 tgccaccgta agtttatcgc ccggtttcgc ctgcatcgcc cagccgctgg cgaccccacc
    32581 gtcgtgaata aaaaaatcaa tcgccagttc atggcgtagt tcgtcataca gcggcgtata
    32641 gtcacgcgac ggtgggcgtg atccttccgg ccagacgatg ccctcttccg ttaccgttgg
    32701 cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg
    32761 cgatgtaaaa ccgtccagcg cctcgccgcc gaggacaatg cgctgaaaac cggcgctgat
    32821 gcgctcaacg cgtagcacag tcagttcacg gaagcgcaga tcattgcgaa cgcgctgtgg
    32881 gtagcggggg gtgttattca ttgttatcgc cttcgtgatg gtaatcagat atatctaaat
    32941 aaattccaca aatgataatg attgttaatc atgataaatg caagtgattt gtagcactga
    33001 tatatct
//
LOCUS       DAJOTB010000040        32621 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJOTB010000040 DAJOTB010000000
VERSION     DAJOTB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.040"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(108..3554)
                     /gene="mfd"
                     /locus_tag="OHN28_001853"
     CDS             complement(108..3554)
                     /gene="mfd"
                     /locus_tag="OHN28_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCQ2297256.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3681..4754)
                     /locus_tag="OHN28_001854"
     CDS             complement(3681..4754)
                     /locus_tag="OHN28_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCQ2297257.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            5015..6214
                     /gene="lolC"
                     /locus_tag="OHN28_001855"
     CDS             5015..6214
                     /gene="lolC"
                     /locus_tag="OHN28_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCQ2297258.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6207..6908
                     /gene="lolD"
                     /locus_tag="OHN28_001856"
     CDS             6207..6908
                     /gene="lolD"
                     /locus_tag="OHN28_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCQ2297259.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6908..8152
                     /gene="lolE"
                     /locus_tag="OHN28_001857"
     CDS             6908..8152
                     /gene="lolE"
                     /locus_tag="OHN28_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCQ2297260.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8181..9092
                     /gene="nagK"
                     /locus_tag="OHN28_001858"
     CDS             8181..9092
                     /gene="nagK"
                     /locus_tag="OHN28_001858"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCQ2297261.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9108..9929
                     /gene="cobB"
                     /locus_tag="OHN28_001859"
     CDS             9108..9929
                     /gene="cobB"
                     /locus_tag="OHN28_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCQ2297262.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10067..10855)
                     /locus_tag="OHN28_001860"
     CDS             complement(10067..10855)
                     /locus_tag="OHN28_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCQ2297263.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(10852..11313)
                     /locus_tag="OHN28_001861"
     CDS             complement(10852..11313)
                     /locus_tag="OHN28_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HCQ2297264.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            complement(11371..12417)
                     /gene="potD"
                     /locus_tag="OHN28_001862"
     CDS             complement(11371..12417)
                     /gene="potD"
                     /locus_tag="OHN28_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCQ2297265.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12414..13208)
                     /gene="potC"
                     /locus_tag="OHN28_001863"
     CDS             complement(12414..13208)
                     /gene="potC"
                     /locus_tag="OHN28_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCQ2297266.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13205..14062)
                     /gene="potB"
                     /locus_tag="OHN28_001864"
     CDS             complement(13205..14062)
                     /gene="potB"
                     /locus_tag="OHN28_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCQ2297267.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(14046..15182)
                     /gene="potA"
                     /locus_tag="OHN28_001865"
     CDS             complement(14046..15182)
                     /gene="potA"
                     /locus_tag="OHN28_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCQ2297268.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15432..16658
                     /gene="pepT"
                     /locus_tag="OHN28_001866"
     CDS             15432..16658
                     /gene="pepT"
                     /locus_tag="OHN28_001866"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCQ2297269.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(16707..17828)
                     /gene="roxA"
                     /locus_tag="OHN28_001867"
                     /gene_synonym="ycfD"
     CDS             complement(16707..17828)
                     /gene="roxA"
                     /locus_tag="OHN28_001867"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCQ2297270.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVVPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(17904..19364)
                     /gene="phoQ"
                     /locus_tag="OHN28_001868"
     CDS             complement(17904..19364)
                     /gene="phoQ"
                     /locus_tag="OHN28_001868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCQ2297271.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(19364..20035)
                     /gene="phoP"
                     /locus_tag="OHN28_001869"
     CDS             complement(19364..20035)
                     /gene="phoP"
                     /locus_tag="OHN28_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCQ2297272.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(20204..21574)
                     /gene="purB"
                     /locus_tag="OHN28_001870"
     CDS             complement(20204..21574)
                     /gene="purB"
                     /locus_tag="OHN28_001870"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCQ2297273.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21578..22219)
                     /gene="hflD"
                     /locus_tag="OHN28_001871"
     CDS             complement(21578..22219)
                     /gene="hflD"
                     /locus_tag="OHN28_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCQ2297274.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(22255..23361)
                     /gene="mnmA"
                     /locus_tag="OHN28_001872"
     CDS             complement(22255..23361)
                     /gene="mnmA"
                     /locus_tag="OHN28_001872"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCQ2297275.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(23415..23876)
                     /gene="nudJ"
                     /locus_tag="OHN28_001873"
     CDS             complement(23415..23876)
                     /gene="nudJ"
                     /locus_tag="OHN28_001873"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCQ2297276.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(23886..24539)
                     /gene="rluE"
                     /locus_tag="OHN28_001874"
     CDS             complement(23886..24539)
                     /gene="rluE"
                     /locus_tag="OHN28_001874"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCQ2297277.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            24711..25961
                     /gene="icd"
                     /locus_tag="OHN28_001875"
     CDS             24711..25961
                     /gene="icd"
                     /locus_tag="OHN28_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCQ2297278.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(26075..27217)
                     /locus_tag="OHN28_001876"
     CDS             complement(26075..27217)
                     /locus_tag="OHN28_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2297279.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(27207..27443)
                     /locus_tag="OHN28_001877"
     CDS             complement(27207..27443)
                     /locus_tag="OHN28_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCQ2297280.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(27583..27822)
                     /locus_tag="OHN28_001878"
     CDS             complement(27583..27822)
                     /locus_tag="OHN28_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCQ2297281.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            complement(27806..28132)
                     /locus_tag="OHN28_001879"
     CDS             complement(27806..28132)
                     /locus_tag="OHN28_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCQ2297282.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            complement(28132..28353)
                     /locus_tag="OHN28_001880"
     CDS             complement(28132..28353)
                     /locus_tag="OHN28_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCQ2297283.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            complement(28452..28733)
                     /locus_tag="OHN28_001881"
     CDS             complement(28452..28733)
                     /locus_tag="OHN28_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCQ2297284.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            complement(28744..28935)
                     /locus_tag="OHN28_001882"
     CDS             complement(28744..28935)
                     /locus_tag="OHN28_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HCQ2297285.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(28908..29090)
                     /locus_tag="OHN28_001883"
     CDS             complement(28908..29090)
                     /locus_tag="OHN28_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HCQ2297286.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(29087..29767)
                     /locus_tag="OHN28_001884"
     CDS             complement(29087..29767)
                     /locus_tag="OHN28_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCQ2297287.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(29764..30549)
                     /gene="bet"
                     /locus_tag="OHN28_001885"
     CDS             complement(29764..30549)
                     /gene="bet"
                     /locus_tag="OHN28_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HCQ2297288.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(30555..30851)
                     /locus_tag="OHN28_001886"
     CDS             complement(30555..30851)
                     /locus_tag="OHN28_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCQ2297289.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(30927..31217)
                     /locus_tag="OHN28_001887"
     CDS             complement(30927..31217)
                     /locus_tag="OHN28_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297290.1"
                     /translation="MTTGGHPPPEYAQKSLYVQQPSLRAGVFMVHQHYGTQTVNRGAV
                     MPGMLVKHKDGTWTASANLRGRLYLHRGIERTYTRDLLVEVFLDGRGNGLNR"
     gene            complement(32036..>32621)
                     /locus_tag="OHN28_001888"
     CDS             complement(32036..>32621)
                     /locus_tag="OHN28_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297291.1"
                     /translation="MNRTISSLQTYIRWLVGNEMIEFTPMIVKGNKVQLKDIKSISVR
                     DPSPLPKEVLVQSSDMKSPPSEIEEKNTKIKLSHAVLNALKSAVPNFPNLKEIIDNQI
                     VSAELLIKFSKPRSMDEDSYAKLLGATLKPVSDLDNIVFKRSDGRSEIKGKDLLKIKN
                     VTIEVTDSGKLVEQEVFQEMSRYLKEIENEATNS"
BASE COUNT         7944 a         8111 c         8252 g         8314 t
ORIGIN      
        1 aaaggtaagt ctgaattatg gagggtgaca gtgtcggata atgcagggaa gtgcaaagaa
       61 tttgtaaata ttgcagatgg gggcgcagaa acgcccccga tttaccatta agcgatcgcg
      121 ttctcttcca gttcacgcat aaactggcgt acccattcga tacgcgtttt ccgctcgctc
      181 aaatcctgaa taaacttcag acgcgtcgga ccatcaaggc ggtaatgctg cggctgtttt
      241 tgcagcaaac caatcaacca ggccggatta acgtgattct tctcggcaaa ttcgatcacg
      301 ccgcctttct cattaccttc cagcttcctg atccccagtt tctgcgcttg ctggcgcaga
      361 cgggcaatat ccagcagggt acgcgccgga tccggcagca ggccgaagcg atcgataagc
      421 tcgactttga tctcttccag ttcgttttcc gttttggcgc tggcaatacg tttgtagaac
      481 gacagacgcg tgttcacgtc agggatgaaa tcatctggca atagcgacgg catccgcagc
      541 tcgacttctg tttgctggct ggtgagatct tccagcgacg gctcgcgtcc ggctttcagc
      601 gcatcgacgg cgttttccag caactccata tacagcgaga aaccgatggt ttccattgag
      661 ccgctttgtt cttcgccaag cagttcaccc gcgccgcgga tctccagatc gtgcgttgcc
      721 agcgcaaaac ctgcaccgag atcttccagc gaggcaatcg cttcaagacg tttttgcgca
      781 tcggtagtca tcgcttttgg atgcggcgtc agcagccatg catatgcctg gtggtgcgaa
      841 cgcccgacgc gaccgcgcaa ctggtgcaac tgtgccagac cgaagtgatc cgcgcgttca
      901 atgataatgg tgttggcggt aggaatgtcg atcccggttt cgataatggt ggtacaaacc
      961 agcacgttga aacgttgatg atggaaatca ttcatcaccc gttccagttc gcgctcacgc
     1021 atttgcccgt gaccgatagc aatccgcgct tcaggtacca gttctgccag ccgttcggcg
     1081 gctttctgga tgttttccac atcattgtag agataataaa cctgtcctcc gcgcaaaatt
     1141 tcacgcagga tcgcctcccg gaccaccaga ctgtcatact cacggacaaa ggttttaact
     1201 gccagacgac gggcgggcgg cgtggcgata atcgacagat cgcgcattcc gctcattgcc
     1261 atattcagcg tacgtgggat cggcgttgcg gtaagcgtca gaatatccac gttcgcgcgc
     1321 atcgctttaa tgcgctcttt atgacgcacc ccgaagcggt gttcttcatc gacaatcagc
     1381 aggcctaaat ctttaaactt gacgtcactt tgcagcagtt tgtgcgtacc gatcagaata
     1441 tcaattttcc cttccgccac ttccgcaagg atttgcgtct gctctttggc gctgcggaaa
     1501 cgggagatca tttcgatacg taccggccag ttggcgaaac ggtcgcggaa gttgtcgtaa
     1561 tgctgctgcg cgagaagggt ggtaggcacc agcaccgcca cctgtttgtg gttatctacc
     1621 gccatgaaag cggcgcgcat cgccacttct gttttaccaa agccaacatc gccgcacacc
     1681 agacgatcca ttgccagcgg ctgacacatg tcactaagca ccgcattaat ggcctgcgcc
     1741 tgatccggcg tggtttcaaa cgggaagctg tcgcagaaca actgatactg ctcgcgatcg
     1801 tgtttaaacg cgaagccctc tttggcggcg cgttgcgcgt agatatccag caattccgcc
     1861 gccacatcac gcactttttc cgccgctttc tgccgcgcgc gtgaccaggc atcgccacca
     1921 agtttatgca gtggtgcgtt ttcttctgcg ccacccgcgt aacggctaat cagatgcagt
     1981 gacgacaccg gaacatacag tttggcgtcg ttggcatagg tgagcatcaa atactcgcca
     2041 gtaatgcctc cagcttcgag cgtggtcatt ccggcataac gaccgacgcc gtgctccaga
     2101 tggaccaccg gctgaccaat atgcagctcc gcaaggttac ggatcagtgt atcggggttg
     2161 atggcgcggc gagaatcctg acgacgacgg gcaacgcgtt caccgagcag atcgctttcg
     2221 caaatcagcg ccagattacg taccgtatcg acaaaaccat gctctgcagc gccaatcatc
     2281 agataacgcc cacggtcgct ggcttcatca agacgcataa tgcgttgcgg agcaatttta
     2341 attcgcgcga gcagttcacc caacgcttca cggcggcctt cactttctac cgagaacacc
     2401 accggaccgt cgaaagactc gaggaactta cgcagcgcat ccagcggcgc tttttgttgt
     2461 gcctgaatgg ccaggtctgg cagtttctgg aaacctaaat tggcattcgc tgcttttgtc
     2521 ggtaaatgtt cagtttttag ctgcacacgg ggccagtttt tcagctctga gaagagctca
     2581 tccacccgca gccagagcga ttgtggtggc aacagtgggc gcattgggtc gatgccgcga
     2641 ttctcaaaac gcgcgagcgt gtcagcctgg aaacgttcgg cactgttttc cagatcgcca
     2701 gtattcacca acagcgtatt ggcagggaaa taactgaaca gtggcggcag cggttcgctg
     2761 aagaatagcg gctgccagta ctcgatcccg gcaggtaatg tgcctttact cacttgctga
     2821 taaatatgtt ccgggtcgcg cttcacttcg aaggtatcac gccactggct gcggaacagt
     2881 tcaattgccg ctttatcggt cggaaattcg tgcgcgggca acagattgat cgcttctact
     2941 tcctccagcg tgcgctggct atcgacgtca aacacccgca ggctgtcgat ttcatcatca
     3001 aagaaatcaa gacgataagg cagctcactc cccatcggga agagatccaa caacgcgccg
     3061 cgcgtggcgt attcgccgtg ctccatcacc tggtcaacat ggcgataacc ggcgctgtcc
     3121 agttgggttc gtaatgcatc tcgtgacagg cgctgacctt ttttcatcac cagcgcatga
     3181 ccgtggagaa agctgtgcgg gcaaacgcgc tgcataagcg tattcaccgg aacaatcagt
     3241 acgccacgct gcatcgatgg taactggtaa agggtggaaa gacgcgagga gataatgtcc
     3301 tgatgaggcg aaaaactgtc gtaagggagg gtttcccagt ccgccagatt catcaccatc
     3361 tgatcggtga actggctgat ttcatcatgc aaacgcagag cattttgcat atctggtgca
     3421 atgagtacca ccggaccggc gtgacgttcg gcaatttctg ctaccagcgt cgcgcaggca
     3481 gcgccggtta actcgcccag cagacgctgc tcacccgctt tgacgggcag cgtataacga
     3541 tattgttcag gcataacggt tgtcagattc tcgttaggat atgcctcaac atatggggca
     3601 tatctctgat aagcaatgct gttcattatc cgtaatcgac atggtttagc aaattgaatc
     3661 gccccggctg gggcgatggc ttagcgggag taactgacgg tagcgggcag agaaggcggg
     3721 gagaacaaca gatcgctgaa tgcgcgctgt gaaagttttc ttaccagcaa gcccgcaaca
     3781 gtacaaatca acaaactgac aaaggggtaa atcagcagga gcgtcagttc gacttgcggc
     3841 gaccagcgta ccgcgttcat ttgcggaatc aggcttaagc tgaatatttc taccagaatg
     3901 cgatgggtgg tatagatagc aatggtgttg gagccaatca cattcagcag gctggaggaa
     3961 cgcataccga aacgctgctc gtactgataa aacagcttca tgattaccac aatcgatacc
     4021 agcgacaaca gcagcgagat attaaacaac caggcaccga ccgccagaac ggtcagcaaa
     4081 gaagccatca gcagatggcg gcgcaacggt accgctttaa cacaggtcat taatgttgcg
     4141 ccaaaccatg cgccaaggct gtaataaggc aaattgcgga tcacactgtt cattccccac
     4201 cacggtgtgg gtacgaaatt aacagccaca ctcatcagta caaacagaac aaataacggc
     4261 agggccaggg ggttaaaaat tttacatatc acgaaataga caattaacgc atacagatac
     4321 cacaagctgg tgctggcggt gatcatcccg tgcaggaact caccggtgga atcggcataa
     4381 gcggcattgg aggcattgct taaatcgcgc tcaggtgcca gccactggtt cagcgcactt
     4441 aacgccagcc actgcaccac gccccaaagc gccagcaccc agaagatgtt ccagatgcgt
     4501 ttatcgagac aatttcccca cggtacgctg tcgatatagc ggcgaatcaa atagccagag
     4561 ataaagaaaa aaaccggcat acgaaagggt gcaaggtaaa gattgaaata gatccagcat
     4621 ttgctcagga cttccgataa cggatgctga aaagtggtca gatgcggata aaaggtaatg
     4681 accgagtgat aaatcaccac cagacaaata cataaccctt tgatctggtt aatccatagc
     4741 tctttttgtt tcatcgggac aacacgctct ttttatttaa catgcggttg atcctgcggg
     4801 gcgagtcgcg tggtgtaatg cgtatctgtc tgtatttggt tatttttcag ataaagatca
     4861 ggccacatgg ccgatgataa aatgaggcac tttagccgat tgatttgctg gtacttttca
     4921 gattagccct gacgatcact tacagttcag acgtttaccc atcctgcttt cgcttatata
     4981 ctcgtgtctt tgctacagca accagacgga tttcatgtac caacctgtcg ctctatttat
     5041 tggcctgcgt tacatgcgtg ggcgtgcagc ggatcgcttc ggtcgtttcg tctcctggct
     5101 ttctaccatc ggcattaccc tcggggtgat ggcgctggtc acagtattgt cagtgatgaa
     5161 cggctttgag cgcgagctgc aaaacaacat ccttggcctg atgccacagg caattctctc
     5221 ttctgagcat ggctctctta acccgcagca actcccggaa acggcagtca aactggacgg
     5281 cgttaatcgc gtcgcaccta ttactaccgg tgatgtggta ctgcaaagcg cgcgcagcgt
     5341 ggcggtcggg gtgatgctgg gtatcgaccc ggcgcaaaaa gatccactaa cgccgtatct
     5401 ggtcaatgtg aaacaaactg acctcgagcc ggggaaatat aatgtcatcc tcggtgaaca
     5461 gcttgcctca cagttgggcg tcaatcgcgg tgatcaaatc cgcgtgatgg tgccatctgc
     5521 tagccagttc acgccgatgg ggcgtattcc aagtcagcgc ctgttcaatg tgattggcac
     5581 tttcgccgcc aacagtgaag tcgatggcta tgaaatgctg gtgaatattg aggatgcctc
     5641 gcgtctgatg cgttatccgg caggcaatat taccggctgg cgtttgtggc tggatgagcc
     5701 gctgaaagtc gactcattaa gtcagcaaaa actgcctgaa ggcagcaaat ggcaggactg
     5761 gcgtgaccgt aaaggcgagc tgttccaggc cgtacgcatg gaaaaaaata tgatgggctt
     5821 gctgctgagc ctgattgtcg ccgttgcggc gtttaacatt attacctcgc tgggactgat
     5881 ggtgatggag aagcagggcg aagtggccat tcttcagact caggggctga ctccgcggca
     5941 aatcatgatg gtctttatgg tgcaaggggc cagcgccggg attatcggtg cgatcctcgg
     6001 agcggcgctt ggcgcactgc ttgccagcca gttgaataat ctgatgccga taatcggcgt
     6061 cctgcttgat ggcgcggcgc tgccggtcgc tatcgagcct ttacaggtca ttgttattgc
     6121 gctggtggcg atggctatcg cgctgctgtc tacgctttac ccttcctggc gcgctgccgc
     6181 cactcaaccc gctgaggctt tacgttatga ataagatcct gttgcaatgc gacaacctgt
     6241 gcaaacgcta tcaggaaggc agtgtgcaaa ccgatgtact gcacaatgtc agtttcagcg
     6301 tgggcgaagg tgaaatgatg gcgatcgtcg gcagctctgg ttccggtaaa agcaccttgc
     6361 tgcacctgtt aggcgggctg gatacgccaa cctccggcga tgtgatcttt aacggtcaac
     6421 cgatgagcaa actgtcttcg gcggcgaaag cagaactgcg caaccagaag ctgggattta
     6481 tttatcagtt tcaccacctg ctgccggatt ttaccgccct ggaaaacgtg gctatgccgc
     6541 tgttgattgg caagaaaaag cccgctgaaa tcaacagccg tgcgctggag atgttaaaag
     6601 cggtggggct ggagcatcgt gcgaatcacc gcccatctga actttctggc ggcgaacgcc
     6661 agcgtgtggc gattgcccgt gcgctggtca ataacccgcg gctggtactg gcggatgaac
     6721 ctactggtaa cctcgatgca cgtaacgccg acagcatctt ccagttgctt ggggaattga
     6781 atcgcttgca gggcaccgcc ttcctggtgg ttactcacga cctgcaactg gcgaaacgta
     6841 tgagccgcca actagaaatg cgtgatggac gtctgacggc ggaactgagc ctgatggggg
     6901 cggagtaatg gcgatgcctt tatcgttatt gattggcctg cgttttagcc gcggacggcg
     6961 acgcggcggc atggtgtcgt tgatctccgt catttctacc attggcattg ccctcggcgt
     7021 ggcggtattg atcgtcggct taagcgcgat gaacggcttt gaacgcgaac tgaataaccg
     7081 cattctggcg gtagtaccgc acggtgaaat tgaggcggta gatcagccgt ggaccaactg
     7141 gcaggaagca ctggataacg tgcagaaagt gccgggcatt gccgccgctg cgccgtatat
     7201 caatttcacc gggctggtgg aaagtggcgc gaatctgcgc gcaatccagg tcaagggcgt
     7261 taacccgcaa caggaacagc gtttgagcgc attaccctcg tttgtccagg acgatgcgtg
     7321 gagcaatttt aaagcaggcg aacagcaaat tatcatcggc aaaggcgtgg cggatgcgct
     7381 gaaagtgaag cagggggatt gggtgtcgat tatgatccca aattcgaatc ccgagcataa
     7441 actgatgcag ccaaaacgtg tgcgtttgca cgttgccggt attttgcagt tgagtggtca
     7501 actcgatcac agttttgcca tgatcccgct ggccgatgct cagcaatatc ttgatatggg
     7561 ttccagcgtg tcaggtattg cccttaaaat gacggatgtt ttcaacgcca ataagctggt
     7621 acgcgatgcg ggtgaagtga ccaacagcta tgtttatatt aaaagctgga ttggtactta
     7681 cggctatatg tatcgcgata ttcagatgat ccgcgccatt atgtatctgg cgatggtact
     7741 ggtgattggt gtggcctgtt tcaacatcgt ctccacctta gtgatggcgg tgaaagacaa
     7801 gagtggcgat atcgcagtat taagaacgtt gggtgcgaaa gatggtttaa tccgcgccat
     7861 ctttgtctgg tatggattgc tggcagggct gttcggcagc ctgtgtggtg tgattatcgg
     7921 cgtagttgtt tcactgcaac ttaccccgat tattgagtgg attgaaaagc tgatcggcca
     7981 tcagttcctc tccagcgata tctattttat tgacttcctg ccatcggaat tgcactggct
     8041 ggacgtcttc tacgtactgg tcacagcatt gttgctgagt cttttggcaa gttggtatcc
     8101 ggcgcggcgc gccagtaata ttgaccctgc gcgagtcctt agcggccagt aaaggcagta
     8161 cattaaaaca aggagcggca atgtattacg ggtttgatat tggtggaaca aaaattgcgc
     8221 taggcgtgtt tgatagcggt cggcagttgc agtgggaaaa gcgggtgccg acaccgcgtg
     8281 acagctatga cgcattttta gatgcagtgt gcgagctggt agccgaagct gaccaacgtt
     8341 ttggctgtaa aggttctgtc ggcattggta ttccgggtat gccggaaaca gaagatggta
     8401 cgctgtatgc cgccaatgtc cctgctgcca gcggtaaacc gctgcgtgcc gacctgagcg
     8461 cacgtcttga tcgcgatgta cgccttgata acgatgccaa ctgttttgcc ctttctgaag
     8521 catgggatga tgaatttact caatatccac tggtgatggg gttgattctc ggcaccggcg
     8581 ttggcggcgg gctgattttc aacggtaaac caattaccgg taaaagctat attaccggcg
     8641 agtttggcca tatgcgtctg ccggttgatg cgttaaccat gatggggctg gatttcccgt
     8701 tacgccgctg cggctgtggt cagcatggct gcattgaaaa ttatctgtct ggtcgcggtt
     8761 ttgcgtggct gtatcaacac tattatcatc aaccgttgca ggctcctgag atcattgcgc
     8821 tttatgatca aggcgatgag caggcaaggg cgcacgttga gcgttatctg gatttattag
     8881 cggtttgtct gggaaatatc ctgaccattg ttgaccctga cctggtcgtc attggtggtg
     8941 gcttatcgaa tttcccggca atcacaacgc aactggcgga aaggttgcct cgtcatctct
     9001 tacctgtagc tcgtgttccg cgcattgaac gcgcgcgcca cggtgatgcg ggaggaatgc
     9061 gtggtgcggc cttcctacat ctaaccgatt aatcaacaga ggttgctatg ctgtcgcgtc
     9121 ggggtcatcg gttaagtcgt tttcgtaaaa ataaacgccg cctgcgcgag cgtttgcgtc
     9181 agcgtatttt tttcagagat aaagtggtgc cggaagcaat ggaaaaacca agagtactcg
     9241 tactgacagg ggcagggatt tctgcggaat caggtattcg tacctttcgc gccgcagatg
     9301 gcctgtggga agaacatcgg gttgaagatg tggcaacgcc ggaaggtttt gatcgcgatc
     9361 ctgaactggt gcaaacgttt tataacgccc gtcgtcgaca gttgcagcag ccagaaattc
     9421 agcctaacgc tgcacatctt gcgctggcga aactgcaaga tgctctcggc gatcgctttt
     9481 tgctggtgac gcaaaatatc gataacctgc atgaacgcgc aggcaatacc aatgtgattc
     9541 atatgcatgg ggaattgctg aaagtacgtt gttcgcaaag tggccaggtc ctcgactgga
     9601 ccggagacgt tacgccagaa gataaatgcc attgttgcca gttcccggca cccttgcgcc
     9661 cgcacgtagt gtggtttggc gaaatgccac tcggcatgga tgaaatttat atggcgttgt
     9721 cgatggccga tattttcatt gccattggta cttcggggca tgtttatccg gcggctgggt
     9781 ttgttcacga agcgaaactg catggcgcgc acaccgtgga actgaatctt gaaccgagtc
     9841 aggttggtaa tgaatttgcc gagaaatatt acggtccggc aagccaggtg gtgccagagt
     9901 ttgttgaaaa gttgctgaag ggattataaa gcgggaaaaa ttgcctgatg cgctacgctt
     9961 gtcaggcctg tgaaggggcg caattaattg aatttgcggg ttttgtgggc cggataaggc
    10021 gttcgcgccg catccggcat aatacacgcg aactttactg cctcttttac cgtttactcg
    10081 tccatagtaa cgcggcaata aacgtacaga caccaatcag ccctgcccag agtggcgata
    10141 ttctggtaat cgcaaagctg ttatcgatat acaaacataa agcctgataa cccatattgg
    10201 caacagccat cgccactgca aattttaacg cataataaag gcgcgttgtg tagttaaaaa
    10261 atgcagcaaa gacgatgaat gcaactgccc aggcaaaaag gcctaatgtc gtggtactgc
    10321 cgggtggatc attttcatgc tgttgtccgg gatgcggata atatgtcagt ggtggcgtcg
    10381 gttgttttaa tatctgtgct tgtaaaacag ccaatgcatt attgataccc tgataccaga
    10441 ggtccgcatg taaataagcc agcgtgtgat tttctaccag aaggccgaga tgtttatcgt
    10501 ccagaccgct ttcaacctgg ccgttggtta tcataattaa ccgtggctcg cgggtagtaa
    10561 agagtaacag aacacttcct tttacattac tcattgaaga tgccgcatgc gccagcatcg
    10621 ctttgccatt ttgcctgaga ctggcttcat tattaatgtc gggtaaaacc acaataaacg
    10681 gtgcaactcc ggtttgttgg cgaaaaagct cggctttatg cgcgatctct tcagcatctg
    10741 gtacagtacc ggtaacatcc gtaaaaagtg tcttcattat tggaattaag ggcttactgg
    10801 ttgccgtcag aggcaggagt atcagtaaac tgagcaatat tatgattttt ttcatgggcg
    10861 ggacttccgt ttcctgcgtg tctttttaga ggagggcata ctgattaata aaccgacgga
    10921 gataaaaata acgcccataa tgccgataaa cccccattta aaaaaattaa cccacgggtt
    10981 atttatctca gccttttcag gatgtcctgg agaataataa acattaactt tatcgccttc
    11041 gctgccttca taaaaatcgc tgccaatact cgaattgaaa attagcgtgt aatctggcgt
    11101 cggacgaaag gcaacgacgg gataccacga accattttta cccgtgcgat gagaatgact
    11161 ggagtgccag acggtatcaa caatgacgcc ttcagtatga atcgcgtctt tgctataggt
    11221 gaactggtta taaaagataa caacagaaat gaggatcagt accagaccga taccgaagaa
    11281 tatacgaaag aaaactctac tgtcctggct cattagccac atccttgcta aataaaaaac
    11341 gggcggtaat atcaccgccc gcttgctgaa ttaacgtcct gctttcagct tctgataata
    11401 ctcttcataa atgctgctgg ctgcgccaac gtcattctgc cattcaccat ttttaatggt
    11461 ttcagcatcc gggtagagcg ttttatcgtt cgccacttcc ggacttaaca gcttacgcgc
    11521 cgcaaggttt ggcgttggat aaccgatagt ttcagcaacc tgttttgcca catccgggcg
    11581 cagcaggaag ttgatcaatt tcagcgcgcc ttctttgttt ttggcatttg ccgggatcgc
    11641 caggctgtcc atccagaaaa taccgccttc tttcggccac accacatcaa ttggcgtacc
    11701 cgcctggcgt gcaacgaaag cagaaccgtt ccagatcatg ccgaggttaa cttcgccttc
    11761 catatacggg ttcgccgggt tatcggagtt aaacgccgcg acgtttggca tcagcttttt
    11821 cagctcgttg tatgcagctt caatctcttt cggatcggtg gtgttaccgg agtagcccag
    11881 cttacgcagc gccatctgga acacttcacg ggcatcgtcg gtcagcagca ggctgccttt
    11941 gtactcaggc ttccacagat cggcccagct ggtgacagat ttcggatcca ccgcatcacc
    12001 gttaacgcca atcgccgtcg caccccagat atacggaatg gaatagtcgt tattcgggtc
    12061 aaacggcttg ttgagcatgt ctggatcgag gttgctgaaa ttgcttaact tcgacttgtc
    12121 gatcttctgg atcatccctt ctttacgcat tttatcgacg taataggttg aaggaaccac
    12181 caggtcatag gcaccgtctt tgtacgtttt cagcttcgcg tacatggttt cgttcgactc
    12241 gtaagtcgaa tagataacct taataccggt ttctttggtg aactgttcaa gcagtcctgg
    12301 cggcacgtac tcggtccagt tgtagaaata cagcgtgttg ttgtcatcgg cgtgagcggc
    12361 gctcatgccc agtgccagag cacccgccgc gagcaggtgg cgtgaccatt ttttcattta
    12421 acgtcccctg tgttaccttt cgttttatca cgagcaataa gctggctggc aattaccatc
    12481 accagcgaca gcaccagtaa tatggttgcc agcgcgttaa cttccggcga tacgccgact
    12541 ttgaccatcg aataaatttt taacggcaga atttcataac tcggcccggt gacaaacgaa
    12601 gaaaccacca cgtcgtccat cgacagggta aagcttaaca cccagcccgc cgctaccgct
    12661 ggcattgcca gtggcagaat gatttttcgc agaatggtaa attcgctggc accgagatct
    12721 ttcgccgctt ccagcattcg cacgtcaaaa cctttcaggc gcgaatacac tgtcactacc
    12781 acaaatggca ggcagaaggt gatatgcgag aacagcagcg accagaagcc aagctgaata
    12841 cccagcagca taaacagcac cagcagcgaa atcgccatta cgatatctgg tgacatcatc
    12901 accacaaaca gcattccgct aacgaacggc ttaccacgaa agcgataacg gtacagcgca
    12961 actgccgtca gtgaaccgat aagcgtagca aacgtcgccg aaaacaccgc cattgtaagt
    13021 gaatgctgcg ctgcctgtaa caggctgtcg ttgttcatca gcaggctata ccatttggtg
    13081 gtaaaaccct gccagttgat gccaaagcgc gagctgttaa aggagttcac aatcaaaata
    13141 atgattggga tatacaggta cgcgtagata gcggtcataa aaccgccgcg aagcagtcga
    13201 ccgatcattc gagttccacc ttcttattca gcaaacgaga agcgcgccag taaaccagca
    13261 acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac ggccagtcac
    13321 gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc gcaccgccca
    13381 tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga catccggcaa
    13441 taattcccgg catcgtcagt gggataatga tgcgaataaa ggtctgcaac ttgctggcac
    13501 cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg gagtacagcg
    13561 gcatcaccat aaacggcagc agaatgtaaa ccagaccgat aatcaccgca ctaggcgtga
    13621 acatgatgcg aattggtgta tcgataacgc ccagccagag caaaaactcg ttgagatagc
    13681 ctttggtgct gaggaaaatt ttcagcccgt aaatacgaat caatgagttg gtccagaacg
    13741 gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc gccagaaacc
    13801 aggcaaaagg atagcccagc accaggcagg cgagggtggc gatcagcgcc atattcagcg
    13861 agtgcaatag cacttcaaaa tagagcggat cgagcagacg cgtgtagtta tccagcgtaa
    13921 agaccatttt gacgaaactg gcgtcgtcgc gggtcaaaaa gctggtgcca atgatcatca
    13981 ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc actacattct
    14041 ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac ccaattaatg
    14101 gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa gaattcgctg
    14161 accatcacca tcttgccatt ttccagctca acaaccgact ccagcgtcat gcctttgtag
    14221 ttacgctcgc gaacgtaacc aatcagccct tcggcgtggt tgtcgtcgtt aatctcttca
    14281 acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc aacggcgaag
    14341 ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg ctcgtcgaga
    14401 cgttcgatga cagtggcgtt aaacatattg atttcgccaa taaacccggc aacaaacagg
    14461 tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg accatcgcgc
    14521 atcaccacga tcctgtccga catggtaagt gcttcttcct ggtcgtgagt tacgaacacg
    14581 aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt tctgcatttg cttacgcagt
    14641 ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt gttaaccacc
    14701 gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg tttgcgttga
    14761 gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt aatttcagct
    14821 gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt catgtggggg
    14881 aaaagtgcgt agctttggaa aacagtgttc acatagcggt tttccgccgg aacgtgggtg
    14941 atgtcctcgt tatccagcat gatgcgtccg gaatcaacag tttccagacc tgcaatcagg
    15001 cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa ctcgccattg
    15061 ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt gcgaattccc
    15121 gccaattgca ccagcggtga aagcgaactc ggttgtttat tcaatttttt actctgtccc
    15181 atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc ttggtgactc
    15241 ttaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg ttgtgaatta
    15301 ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt gcagggctaa
    15361 aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc acaaaaagtg
    15421 agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct cttgataccc
    15481 aatcaaaggc aggggtgaga caggttccca gcacggaagg ccaatggaag ttattgcatc
    15541 tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt gaccttaagt gagaagggca
    15601 ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt ggctttattt
    15661 ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt gaatccgcaa attgttgaaa
    15721 actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca ccggttatgt
    15781 tcccggtgct gcatcagcta ctgggtcaga cgctgattac caccgatggt aaaaccttgt
    15841 taggtgccga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg gtattgcaac
    15901 aaaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa gaagtgggca
    15961 aaggggcgaa acattttgat gttgacgcct tcgatgcccg ctgggcttac accgttgatg
    16021 gtggtggcgt aggtgaactg gagtttgaaa acttcaacgc cgcgtcggtc aatatcaaaa
    16081 ttgtcggtaa caatgttcac ccgggcacgg cgaaaggagt gatggtaaat gcgctgtcgc
    16141 tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg acagaaggct
    16201 atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc gatatgcact
    16261 acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa atgatggaga
    16321 tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaactg gtgattgaag
    16381 acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc gatatcgccc
    16441 agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc ggcggtaccg
    16501 acggcgcgca gttgtcgttt atgggattac cgtgcccgaa cctgttcact ggcggttaca
    16561 actatcatgg taagcatgag tttgtgactc tggaaggtat ggaaaaagcg gtgcaggtga
    16621 tcgtccgtat tgccgagttg acggcgcaac ggaagtaagc gaaaagggat gcggcatgtg
    16681 atgccgcatc cggcttaaat ccaaacttac ccttcgaaga accagtatcc gctattgacc
    16741 agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa attctccgcg
    16801 gtcagcgcaa tgttgctggc gagcgcatcc agtgccggac ggtgcgggga atcgatcttc
    16861 tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccccc caggcgcacc
    16921 agcacatcac cttgtttcag cgcatcgtag atttcatccg gctgataagg cggctctggc
    16981 ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca ttgcttaaaa
    17041 tgttctggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat ctcttgcggc
    17101 agaacgtccg caggatgagc gcgaggtgga acatcaggat cgctgtagta gttgccgccc
    17161 agttcacgtt gcagcacata atcggcaaat ccgctaatca gttcccgcgt atttggcgcg
    17221 cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg cgggaatcct
    17281 ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc ttcgaacgga
    17341 acgacctgta acagatccgg gtgagggcag tgctgtttca tttgcagctt ttcgcccact
    17401 cgccagcgac gacgaccggt accctgaatg ataaacacgt cgtactgatc gagatgcggg
    17461 ccgacgccgc cgcccggtac agaaaaagag atcatcagat catcaatacg ccagtccggt
    17521 agttcacgga acggtcgcat tagcgcggcg gtcggctcat gccagtggtt cactgcttgc
    17581 actaacaatg accagttagt ttcaccgaga tgatcgtagc tttcgaatgg accgtggctg
    17641 acctgccatt tgccatcctg atgactgacc agtcgactgt cgacttcgct ttccatcgcc
    17701 aaacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc gcgttttaac
    17761 accaccggac gtttctgcca gtgacgttca agaaaatcgg gccagttaag agtgagttgg
    17821 tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg atgcttaacg
    17881 taatgcgtga agtatggaca tatttattca tctttcggtg cagaatgctg gcgaccaaaa
    17941 atcacctcca tccgcgcacc gcccagcatg ctctctccgg cgacgatttt accctcatat
    18001 tgctcggtga tttcgcgggc taccgccagc cctacacctt gcccagggcg taaagtatca
    18061 acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca
    18121 tcctcgacca caatatagag atgctcgtct gtttgccttg cagatatttc gacaaactcg
    18181 aggcaatatt tacaggcatt atccagcacg ttgcccatca cctcgacaaa atcgttctgc
    18241 tcaccgacaa agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga
    18301 tacactttgt tcagcgccga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg
    18361 cggctaagca atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc aatttgctgt
    18421 gaaatgcggc tgatttgctc cagcattacc ggctcagcat cactgacgct catcttctca
    18481 ctacgcagag aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc
    18541 aggtcggtga gcgtcgtacg atatttgtcg taacgttcgc gttcactttt taacagtcgg
    18601 ttcaggtttc gtaccagact agtcagttcc cgcgttgtgg ctggattgag caattcgcgg
    18661 ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac
    18721 caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag cacatagata
    18781 aaccagctcc agaccatata ggaacttttt agctccaccg gaatggtatc caccaccaca
    18841 atggttaact ttggcatccg cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc
    18901 atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct
    18961 ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accgttcgat
    19021 tttagccagt cgggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa
    19081 agctgcccgt tctcatcata aattagtgtc atcgtggggc tttgcttgtc gatattttcg
    19141 ggtaactcga catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc
    19201 tcgccacgta acagccgaaa cgtggtttta tcgaaactga cgctataacc gatcagcgca
    19261 accattccgt aggcaagcga aagcaccaat actactgctg ccgttgctaa cagaaaacgt
    19321 acccgcagcg agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca
    19381 gatagccctg gccgcgaacg gtggtaatca cttcttgcgg atattgtgcc tgaatttttt
    19441 tgcgcagacg tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccgggt
    19501 agagttgtag cattaacgaa tctttgctga ccactttgcc attattgcgt atcaacgttt
    19561 ccataatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac
    19621 ggcgggagag atcaacctga aacggtggta gcgaaatgac ctgtgaagcc agaccgctat
    19681 tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca
    19741 cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctc tcacgggcgg
    19801 ttaataccag aatcggcagt gaaacatcat tgctacgcca gcggcgaatc agtgacagac
    19861 catcctcgtc aggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat
    19921 aataatcggc ttctttggca tcttctgcgt catcgacctg atgaccagca tcctgtatct
    19981 gaactttaag atggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta
    20041 tttctccctg tcttaattat taaaatagtg taacgcgatt atgtggttat gggggtaaac
    20101 attaaataaa ccagcgggaa ggggaggtaa agtgaaaaaa taaaaagcgg ataatcttaa
    20161 taagcaggcc ggacagcatc gccatccggc actgatttga ggcttatttc agctcatcaa
    20221 ccatggtgat ggcgcgacca atatagttag ccggcgtcat cgctttcagg cgggctttct
    20281 cttcttctgg cagcgcgaga ccgtcgataa attgcttcat gccttcggcg tcaacgcgct
    20341 taccgcgagt cagctctttc agcttctcgt acggtttttc gatgccatag cgacgcataa
    20401 ctgtctggat tggctcagcc agcacttccc agttgtgatc cagctcctcc agcagatggt
    20461 catggttcac ttccagtttg ctcacgcctt tcagggtgga ttgatacgca atcagcgcat
    20521 aaccgatacc cacgccgagg ttacgcagca cggtggagtc ggtcaggtca cgctgccagc
    20581 gggaaactgg cagtttgctt gccaggtgct gcaataccgc gttggaaagg cccaggttcc
    20641 cttcggagtt ttcgaagtcg atcgggttaa ctttatgcgg catggtggaa gaaccaatct
    20701 caccagcaat ggttttctgt ttgaagtggt taagggcgat ataaccccag acgtcacggt
    20761 caaagtcgat cagaatggtg ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt
    20821 cgtgcggttc gatctgggtg gtgtacgggt tccactgaat acccagcgag gtgacgaact
    20881 cttcgctgaa ctgatgccag tcaacttccg ggtaagcggc gatgtgggcg ttgtagttac
    20941 cgacagcgcc gttgattttg ccgaggatct ccacctggtt aagctggcgg tactggcgct
    21001 ccatacggta ggcgacgtta gccatctctt taccaatggt tgacggcgtg gctggctgac
    21061 cgtgggtacg ggacagcagc gggatatcgc gatactgaac ggcgagatct ttaatgccat
    21121 caatcagttg acgccagtac ggcaggatca cttcatcacg cgcggttttc agcattaatg
    21181 cgtgggagag gttattgata tcttccgaag tacaggcaaa atggatgaat tcagaaaccg
    21241 cgtgcagttc cgggatatcc gccacttttt ctttcaggaa atactcaacc gctttaacgt
    21301 cgtggttagt ggtacgctcg atggttttga tgcgtgctgc atcttcttcg ctgaaactgg
    21361 cgacaattgc atcaaggtaa ccgattgcgt cggcagcaaa agcaggaact tccttgatcg
    21421 ctgcgtgcgc ggccagtttt tgcagccaac gtacttcaac ttgtacacgg aatttcagca
    21481 aaccgtattc gctgaaaatc ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga
    21541 caggggaaac ggcggtcagt gaggataatt ccatagatca caactccggg gttaaatgag
    21601 caagaatttg ttttgcctga gtggtcaggc gattacgaga aaacatcagt tgcagacgac
    21661 cgccgccgac ctggtgccag agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa
    21721 ctttcgcctg cacctgtggg ctttgcagta cagcagggga acccgtgacc tgaatgcgcg
    21781 ggccaagcgg gctgataaca tcaacataga tggcagccat cgcgctcatc agcgtttcgg
    21841 actgtaaatc gaagtgttcg agctggcgtt gcaggccgtt gatccggttg cccagagtgt
    21901 cgagcgcacc tttcgctgag gagagtttgc gctcaagcac catcagactg agtgtgtagc
    21961 gggttaattc ggcgtttaag ccttggcgac tgctggcatt gagtacgccg agcaaggttt
    22021 ccagcccgac gcgcaggttg gcttcgctgc cgccaaaaac cgccagcgtt gagctggggt
    22081 tcatatcgat aatactgttg agtgagacgt gtagcgcatc gccatcacaa tgcccctggt
    22141 gagcgagttg ttgcaccagg cgtgccgact gacaaatacc ggccagggcg agggtgatgt
    22201 catagtaatt ctttgccacg ttcactgctt ccttgtttta agtaaagata ataatcagac
    22261 cggcagcggc agacgctgct caataatacc gccaccgagg cacacttcac cgttatagaa
    22321 aacggcagac tggcccggcg tcacggcggc aaccggctca tcgaaaatca cttcaatgcg
    22381 atcatcgtcc agcgccttga cggtgcacgg gatgtcggtc tggcgatagc gggtttttac
    22441 tgtgcaacgc atagtaccgg tgaacggttc gcgatcgacc cagtgcagct gctgggcaat
    22501 cagaccgaca gacatcagac gcgggtgttc atggccttgc gcaacaatca gaatgttgtt
    22561 ttcgacgtct ttatcgacca cataccacgg ttcttcggtg ccgtctttgg tgccaccgat
    22621 acccagacct ttacgctgac cgagggtgtg atacatcagc ccctggtgct caccaatttc
    22681 atcgccatcg acggtaatga ttttgcccgg ttgcgccggg agataacggc ccaggaactc
    22741 gcggaattta cgttcgccaa tgaagcaaat gccggtagag tcttttttct tcgcggtgac
    22801 cagaccaaga tcttcagcaa tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa
    22861 caggctttgc gcaatctgct catggctgag cgtataaagg aagtagctct ggtctttatt
    22921 gctgtccaga ccacgcagca ggcggctctt gccatcgaca tctgcccgac gtacgtaatg
    22981 accggtagcg atataatcgg cacctaaatc ttcggcggca aattcgagga aagctttaaa
    23041 tttgatctct ttgttgcaca gaatatccgg attcggcgtg cgaccggctt tatattcggc
    23101 aaggaacagt tcgaagacgt tgtcccagta ctcggcagca aagttaacgg tgtgcagttc
    23161 aatgccgagc ttgtcgcaga cagcctgggc atcagccaga tccgctgccg ctgtgcaata
    23221 ttcctcaccg tcgtcttctt cccagttctt cataaacagg ccttcgacct gatatccctg
    23281 ttgttgtaac agccaggcag aaacggagga atcgacaccg ccggacatgc cgacgattac
    23341 tttttttgcg gtttcagaca ttggatcact cacgacattg aacttcaagg cggcgtattc
    23401 tatcacgcag ccttttagat gacacccttt gtaaaaggcc agttaaaatc gccaatcatc
    23461 tccagcggat agcgttgccc gctttgataa caacgaatac tttccgccac cagcggcgaa
    23521 cgaagatttg acgcctgtaa aatttcttcg gcgctgaccc aacggcagca gtcgatatcg
    23581 ctgtcatgag gctgcgtcgg gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa
    23641 aacggcgttt tatcgggcgc aatccactga tgcatacgaa taaagtgttg cggctgcgcg
    23701 ctaatgccgg tttcttccca cagctcacgg gcggcggctt ccactaaggt ttcatcggct
    23761 tccaggtgcc cggcaggttg gttccataac gctttaccat taatcgtctc ttcaacgact
    23821 aaaaatttgc cttctgcgtg caccacgcaa gcaacggtaa cgtgcggttt aaacattttg
    23881 catccttaat ctgtcgcgtc tcgccattcg ccattggcaa gattatccaa agagtaatcc
    23941 cccatcgcat agcgaatcag tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc
    24001 acctggcgat tacgtccttc atataaggtg atcttcagcc agctggtggg aatacttttg
    24061 cgctcacgaa ttggtggatt ccgcggccat aaccacgcgg gttcttcaac cagctccgcg
    24121 cctgcgggca gggtagggcc atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg
    24181 tcttgtgtgg gaataccttc cacctgcaca taatagattt ttccggtgcg tttacccggc
    24241 tgggttaaac gcgcctgcaa cgcgccgtta ttggtcagca ccagtaaccc ttcgctgtcg
    24301 cggtcgagac gacctgccgc atacaccccc tgaaccggga tgaattcttt taatgttttg
    24361 cgtccggctt catcggtgaa ctgcggaaga acatcgtagg gtttattgaa caggatcaca
    24421 cgcgtgggct ggttgtcagg tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc
    24481 tggtgatttc taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt
    24541 atagcctaat aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagctaa
    24601 ttacaaatca ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata
    24661 tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta
    24721 aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc
    24781 ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag
    24841 ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct
    24901 cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gacgtctggt
    24961 tgcctgctga aacccttgat ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga
    25021 ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag gaactggatc
    25081 tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg gttaaacacc
    25141 ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg
    25201 aatggaaagc agactctgcc gacgctgaga aagtgattaa attcctgcgt gaagagatgg
    25261 gcgtgaagaa aattcgcttc ccggaacatt gcggtatcgg tattaagccg tgttcggaag
    25321 aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac gatcgtgact
    25381 ctgtgaccct ggtgcacaaa ggcaacatca tgaagttcac cgaaggcgcg tttaaagact
    25441 ggggctacca actggcgcgt gaagaatttg gcggtgaact gatcgacggc ggcccgtggc
    25501 tgaaagttaa aaacccgaat accggcaaag agatcgtcat taaagacgtg attgctgatg
    25561 cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc
    25621 tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggtggtatc ggtatcgccc
    25681 ctggcgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga
    25741 aatatgccgg acaggacaaa gtaaacccag gctctattat tctctccgct gagatgatgc
    25801 tgcgccatat gggttggact gaagcggctg acctgattgt taaaggtatg gaaggcgcaa
    25861 tcaatgccaa gaccgtaacc tatgacttcg aacgtctgat ggaaggcgct aaactgctga
    25921 aatgttcaga gtttggtgac gcgatcatca agaatatgta atcactacat gtgttaaata
    25981 ttgaaacggg cgtataacac gcccgttgtt ttatgatgat gtaaaatctt ccccaaaact
    26041 ttccccaaaa cccttcccca aaactggcta ttttctatgc tgttttgata tctacgataa
    26101 tccagtcttt accacgatca tcattgtatc ggtcggtcat ttttctggat ttatggccta
    26161 acaacttttg cgtatccaga ccctgttccc gatataaccg ttctgacaga gatcgctgct
    26221 catgaaatgt gggcgcagtt ccttgctccc attttatgcc acatttttcc cgggcctttt
    26281 taaaagccgt tgtcagagta tttgcagaaa cctggtctcc tctgtttgct tgagaggtag
    26341 tgtgacggta atggaccaga tatttactaa caacagcatc cctgcactga gatataactt
    26401 cacgaagggt aatattcaga gcatcgcatt tcaggttaag cggaatagca agttttgaac
    26461 cggttttttc ctgagtaatg tgcaacatgt cgtcccatat atcagagaat ttcaaattgc
    26521 agatatcgcc taaacgttgt ccagtaacaa gagcaagtag catgccgcat tttaaatagg
    26581 gctgccgtct gcttacgctg tcaaatattg cctgccattc gggcagtgac aatctttggc
    26641 ggtttactcg atttcgcggt tgttttgttg cctgcgctgg gttaaatcct ggcggaacat
    26701 gtcctgcgtg ttgtgcttct ttgaagacgt cgatcaacac cattctcacg acttgcgcca
    26761 tcctgttatg accttcagcc tttacagcat caattatttc ggcaatatca agtgcggtaa
    26821 tatccttgag gtgttgcatt ccacaatgct cacggaaaag acgaatgggt ttgccttttt
    26881 gccgatagga gttgggtctt agttcattat gttgcagcct gtcctcttgg atagaaatat
    26941 atttatcaag ccattctgtc accgtaatgt ctgagcgcct gcctttcatt ctttccagac
    27001 gctcattgac gcttaatatt tgtctggtac gttgttcagc aataatggta tttgcttcag
    27061 tagcaacttg ttttgcttca ttctcatcag ttcctaagct atgaaaacga ccggatagtg
    27121 gatgtttgta ttgccaatat acctttccgg ttcgcttatc taatttgcaa tataaattgg
    27181 gtatagagat tttgtgagat cggggtctag cagccatcag cgattatcct ttggagtttt
    27241 gggtttgcgt ttattgggag ttgcggttct gcaagcgttc ctacaaaacg ggaatttcgg
    27301 tcaatcatcc agtagcgacc aacttttata gcgggtgggg ccatcatttt cccttgcgcg
    27361 tattttttca gaactcgctc acttggtgct aagtccccaa attcttcttt agcccagtcc
    27421 tgtaaagtga ttagtcgaga catttgtcct cctcttagct gctgagggag tttgtgaccg
    27481 atatatctga catgatatta agctcatggc aggtacatct cttgactggt catagagata
    27541 aatttaatgc tgagaaatgc agtattgaat ttatcaattt ttctatttcc tgcgcatgtc
    27601 acgtaacttc ttaatgtgtt ctgccgtttc gatctcttct gctatccgat ctgcatcagc
    27661 tttattcaca ggttcaaagt catgattaaa gcggaacatg ctggcgatac atgttctgcc
    27721 ttttcggatg tagtgaactt tgttgtgggt agaacgcagg attttgcagg gagtgccgtg
    27781 gtggtcgacg taccaggtgt tagggtcaat cactctgaac attcttgacc ttgtgttgtg
    27841 cggattgata ttcagtgctg atattcacct ttatagcgaa cacctttacc ggtttatcgc
    27901 caaagtgcgg atgtgtgatt gtcttcactt catatccgtc atacggaaca tcaattctgc
    27961 gactggaatc gtcgcgcttc ggatatccct ttgtgataat caggcggtca tattcccgga
    28021 acataattcg cttattccag tagtcattac acaagcgata ctcttccgtt ttctctccgc
    28081 gaatcatggc atcgaagtat tcacttttaa cagcaagttg caggttagcc atcacttaat
    28141 ccccctttgt ttgcggataa gctccagatc ttgctggcaa ctggcgcaag tccgacaacc
    28201 ctgaacggcc aggcgtcttc gttcatctat cggatcgcca cactcacaac aatgagtggc
    28261 agatacagcc aggtagttca ggcggcgcat ttttattgct gtgttgcgct gtaattcttc
    28321 tatttctgat gctgaatcaa tgatgtctgc catctttcat taatccctga actgttggtt
    28381 aatacgcttg agggtgaatg cgaacaataa aaaaggagcc tgtagctccc tgataatttt
    28441 gcttttcatg ttcatcgttc cttaaagacg ccgtttaaca tgccgattgc caggcttaaa
    28501 tgagtcggtg tgaatccctt cagcgttacc gtttcgcggt gcttcttcag tacgctacgg
    28561 caaatgtcat tgacgttttt atccggaaac tgctgtctga cttttttgat ttcagaatta
    28621 gcctgacggg caatgctgcg aagggcgttt tcttgctgag gtgtcactga acaagcccca
    28681 tgtcggcaag cataagcaca cagaatatga agcccgctgc cagaaaaatg cattcagtgg
    28741 ttgtcatacc tggtctctct catctgcttc tgctttcgcc accatcattt ccagcttttg
    28801 tgaaagggat gtggctaacg tatgaaattc ttcgtctgtt tctgctggta ttggcacaaa
    28861 cctgactcca atttgagcaa ggctatgtgc caaatcaata ctcgttctta actcaacagg
    28921 agatgctttg tgcataccgc cccccgttta ttatttatct cctcagccag ccgctgggct
    28981 ttcagcggat ttcggataac agaaagacca tgaaataccc agcctcgctt tgtaacggag
    29041 tagacgaaag tgatcgcgcc tacccggata ttatcgtgag gatgggtcat cgccattgct
    29101 ctccaaatac aaaaccaatt tcagccagtg cttcgtccat tttttcgatg aactccggca
    29161 ccatctcgtc aaaactcgcc atgtactttt catcccgctc aaccacgaca taatgcaggc
    29221 cttcacgctt cattcgtggg tcatagttgg caaagtacca ggcatctttt cgtgtcaccc
    29281 acatgctgta ctgcacctgg gccatgtaag ccgactttat ggcctcgaaa ccaccgagcc
    29341 ggaacttcat gaaatcccgg gaggtaaacg ggcatttcag ctcaaggcca ttgccgtcac
    29401 tgcataaacc atcgggagag caggcggtgc gcatactttc gtcgcgatag atgatcgggg
    29461 attcagtaac attcacgccg gaagtgaatt caaacagggt tctggcgtcg ttctcgtact
    29521 gttttcccca cgccagcgcc ttagcattaa cttccggagc cacaccggtg caaacctcag
    29581 ccagcagggt gtggaagtag gacattttca tgtcaggcca cttctttcct gagcggggct
    29641 ttgctatcac gttgtgaact tctgaagcgg tgatgacgcc gagccgtaat ttgtgccatg
    29701 catcatcccc ctgttcgaca gctctcacgt cgatcccggt acgctgcagg ataatgtccg
    29761 gtgtcatgct gccaccttct gctcagtggc tttctgtttc aggaatccaa gagctttcac
    29821 tgcttcggcc tgtgtcagtt ctgacgatgc gcgaatgtcg cggcgaaata tctgggaaca
    29881 gagcggcaat aagtcgtcat cccatgtttt gtccagggca atcagcagag tgttaatctc
    29941 ctgcatggtt tcatcgttaa ccggagtgat gtcgcgttcc ggctgacgtt ctgcagtgta
    30001 tgcggtattt tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa
    30061 ggccagacgg gcacactgaa tcatggcttt atgccgtaac atccgtttgg gatgcgactg
    30121 ccacggcccc gtgatttctc tgccttcgcg ggttttgaat ggttcgcggc ggcattcatc
    30181 catccactcg gtaacgcaga tcggatgatt gcggtctttg cggtaaatcc ggcatgtgca
    30241 ggattcgttg tcctgttcaa agtccatgcc gtcaaactgc tggttttcgt tgatgatgcg
    30301 ggaccagcca tcaacgccca ccaccggaac gatgccattc tgcttatcag ggaaggcgta
    30361 aatttctttc gtccacggat taaggccgta ctggttggcg acgatcagca atgcgatgaa
    30421 ctgcgcatcg ctggcatcac ctttaaatgc cgtctggcga agagtggtga tcagttcctg
    30481 tgggtcgaca gaatccatgc cgacacgttc agccagcttc cctgccagcg ttgcgagtgc
    30541 tgtactcatc cgttttatac ctctgaatca atatcaacct ggtggtgagc aatggtttca
    30601 accatgtacc ggatgtgttc tgccatgcgc tcctgaaact caacatcgtc atcaaacgca
    30661 cgggtaatgg cttttttgct ggccccgtgg cgttgcaaat gatcgatgca tagcgattca
    30721 aacaggtgct ggggcaggcc tttttccatg tcgtctgcca gttctgcctc tttctcttca
    30781 cgggcgatct gctggtagtg acgcgcccag ctctgagcct caagacgatc ctgaatgtaa
    30841 taagcgttca tggctgaact cctgaaaatg gctgtgaaaa tatcgcccgc gaaatgccag
    30901 gctgattagg aaaacaggaa aggggattag cgattcaggc cgttaccgcg tccgtcgaga
    30961 aaaacttcca cgagcaaatc acgggtataa gtgcgctcga tgccgcgatg cagatatagc
    31021 cgtccgcgta aattagctga tgcagtccag gtaccatctt tgtgtttgac cagcattcct
    31081 ggcatgaccg cacctcgatt aacggtctgc gttccgtaat gttgatgaac cataaaaact
    31141 cctgcccgta agctgggctg ctgaacatat agagacttct gcgcgtattc aggcggtgga
    31201 tggccgccgg ttgtcataac taagccgcct cgttgaagcg actgaggtat gaagtgttga
    31261 gttgatttca gctggtcaca ccgacgttca cgcgtccgct tcacccctcg cactccccgg
    31321 agcctgctga aattcaagct gcggatctaa gcggtcatcg caacggtgaa tcaggtgatt
    31381 gccgtatcgt tgtgttgttg cgatgaattc atttaaaact atagttgttt tatcgtcaac
    31441 aacaaaagtt gttttgttgg ttgttttaga tataactggt tgtatttaag atggatttat
    31501 tttgtgactt gcatcgcata gcgataactg aagcgaggtc gtggtggttt tttgaacggt
    31561 gtgtgatgag gggagggcaa aagaaaaccc ggcacggtgg ccgggttatt gttcttgatt
    31621 gatcttatcg aagatatcat gacttaattt ttgcacattt aggaaattta ctatgaagaa
    31681 gacaattgcg taaattatta atgtgaaaaa aagtattgaa ggagagaatt ttagtatcag
    31741 ggatttaaat tcaaagctga actcatactc ttttaaatat tcactaagca caaggcataa
    31801 agtagaaaga gtgaaaaagc gcaaaaaaga atttcgtata gataccaatt cttttctgat
    31861 aactttaaca taattaatgt ttttaatgcc agacatgtta aaagttacaa tcaaccctaa
    31921 cccgatagaa aacattatgc cacagacaga atacaatgta gaaatcaaaa actgatcagg
    31981 cctccaagct aaaaatgatg gaagcccgat agtaattatc agaacaatta gcaagctagc
    32041 tatttgtcgc ttcattttct atctctttta aatatctgct catttcttga aatacctctt
    32101 gttctacaag cttgcctgaa tcagtaacct caattgttac atttttaatc ttaagaagat
    32161 ctttgccttt tatttctgag cgaccatcac ttcgtttgaa tacaatgtta tcaagatcac
    32221 taacaggttt taatgttgca cctaatagtt tagcataact atcttcatcc atgctccttg
    32281 gtttactaaa ctttattaac agttcagctg atactatttg attgtcaatt atttctttta
    32341 gatttgggaa atttggtaca gctgatttca atgcatttaa aacagcgtgt gaaagtttta
    32401 tttttgtatt tttttcttca atttctgatg gtggcgattt catatcggat gactgaacca
    32461 atacttcttt tggaagagga gatggatctc tcacagaaat tgattttata tccttaagct
    32521 gaactttatt acctttaaca atcatcggtg tgaattcaat catttcattc ccaaccagcc
    32581 agcgtatata agtttgtaga gatgatatag tcctattcat c
//
LOCUS       DAJOTB010000041        31223 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJOTB010000041 DAJOTB010000000
VERSION     DAJOTB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.041"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            68..3826
                     /gene="yfaL"
                     /locus_tag="OHN28_001889"
     CDS             68..3826
                     /gene="yfaL"
                     /locus_tag="OHN28_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCQ2297292.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDKT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAA
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(3966..4688)
                     /locus_tag="OHN28_001890"
     CDS             complement(3966..4688)
                     /locus_tag="OHN28_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCQ2297293.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4835..7462
                     /gene="gyrA"
                     /locus_tag="OHN28_001891"
     CDS             4835..7462
                     /gene="gyrA"
                     /locus_tag="OHN28_001891"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCQ2297294.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7611..9299
                     /locus_tag="OHN28_001892"
     CDS             7611..9299
                     /locus_tag="OHN28_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCQ2297295.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            9296..9919
                     /locus_tag="OHN28_001893"
     CDS             9296..9919
                     /locus_tag="OHN28_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCQ2297296.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9943..14457
                     /locus_tag="OHN28_001894"
     CDS             9943..14457
                     /locus_tag="OHN28_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCQ2297297.1"
                     /translation="MGTGLANAEDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKDST
                     SHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAP
                     GSEWIGMQVK"
     gene            14458..16107
                     /locus_tag="OHN28_001895"
     CDS             14458..16107
                     /locus_tag="OHN28_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCQ2297298.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKLGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16112..16888
                     /locus_tag="OHN28_001896"
     CDS             16112..16888
                     /locus_tag="OHN28_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCQ2297299.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16962..18146)
                     /gene="atoB"
                     /locus_tag="OHN28_001897"
     CDS             complement(16962..18146)
                     /gene="atoB"
                     /locus_tag="OHN28_001897"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCQ2297300.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18177..19499)
                     /locus_tag="OHN28_001898"
     CDS             complement(18177..19499)
                     /locus_tag="OHN28_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCQ2297301.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19496..20146)
                     /locus_tag="OHN28_001899"
     CDS             complement(19496..20146)
                     /locus_tag="OHN28_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HCQ2297302.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20146..20808)
                     /gene="atoD"
                     /locus_tag="OHN28_001900"
     CDS             complement(20146..20808)
                     /gene="atoD"
                     /locus_tag="OHN28_001900"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCQ2297303.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21004..22389)
                     /gene="atoC"
                     /locus_tag="OHN28_001901"
     CDS             complement(21004..22389)
                     /gene="atoC"
                     /locus_tag="OHN28_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCQ2297304.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22386..24212)
                     /gene="atoS"
                     /locus_tag="OHN28_001902"
     CDS             complement(22386..24212)
                     /gene="atoS"
                     /locus_tag="OHN28_001902"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCQ2297305.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGTLVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24379..27228
                     /gene="rcsC"
                     /locus_tag="OHN28_001903"
     CDS             24379..27228
                     /gene="rcsC"
                     /locus_tag="OHN28_001903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCQ2297306.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27428..28078)
                     /gene="rcsB"
                     /locus_tag="OHN28_001904"
     CDS             complement(27428..28078)
                     /gene="rcsB"
                     /locus_tag="OHN28_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCQ2297307.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28095..30767)
                     /gene="rcsD"
                     /locus_tag="OHN28_001905"
     CDS             complement(28095..30767)
                     /gene="rcsD"
                     /locus_tag="OHN28_001905"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCQ2297308.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7670 a         7625 c         8392 g         7536 t
ORIGIN      
        1 aaaaataccc tatatcgcat aatgcgatta aatacgccat cttatagaaa atgatggtta
       61 gatgaatatg cggattatct ctctacgcaa ggagtattta tctttactcc cgtcaatgat
      121 tgcatctctt ttctctgcta acggtgccgc agcggtcatt gattcatgcc agggatatga
      181 tatcaaagcg agctgtcagg ccagcaggca aagcctttcg ggtaatatac aggactggag
      241 catcgcggat ggacaatggt tgattttttc gggtatggtc aataatgcca gcggtggcgc
      301 agtatttttg cagcacagtg ctgaatttac gatatcacca caaaatgaaa cagggatgac
      361 cctgtttgct gataactcgg ttagtggcga atataataat ggcggggcta tctttgctaa
      421 agaaaactca acgataaatc ttgcgaatgt tattttcgac agtaatgttg caggaggcta
      481 tggcggagca atctattctg ccggtaccaa tgataccggt gccgccgatt tacgagtcac
      541 taacgccgta tttcacaata acatcgctaa tgacggcaaa ggcggtgcaa tttataccat
      601 caataatgac gtctatttaa gtgatgatgc ttttaataat aaccaggcat atacatcaac
      661 aagttatagt gatggggatg gcggcgccat tgatgttacg gataatagta cggataagac
      721 acatctttca ggaaaaacga taattaataa tacctccttt accaataact atgcagaagg
      781 ttatggcgga gcaatttata ccagcagcac cacatccccg tatcttattg atatttctgt
      841 tgatgataat tatgaccaaa acaatggtgt gatgattgat gaaaataaca gcgcctcggg
      901 atatgaccat tcggcgacgg cagcggcggg gggctttatg tatatcggtc atagtgtggc
      961 tgaatttaac attgccgccg ataaaacgct ggtaataggc aataccagca acgatggcgc
     1021 gattgattct cttgccggaa cgggagtcat cgtgaaagag ggagcgggag agttagtcct
     1081 caatgccgac aataatgcgt ttaccggcga gatgagtatc cagaatggcg aggtgacgtt
     1141 ggggcgcagc gatgagttaa tgaatgtcgg cgacacgcac tgtcagagcg atccgcagga
     1201 ttgctttggt ctgatggtcg gcagcactgt tcattctgag tatcaggcag aactgaatgt
     1261 tggcaataca caacaaacgt ttgtgcactc attaaccggt tttgctaatg gcattttgaa
     1321 tatcgacgca ggcggcaatg ttaccgtaaa ccagggcggt ttttccggct cgattcaggg
     1381 tgaaggacag ttgacggtag cgcaagatgg cagctatctc ctgacagggg cgcagtcgat
     1441 ggcattaacc ggcgatattg tggtggaaga taacgccgta ttgtcgctgg cggggaatca
     1501 ggccgattta cgggcaatgc agagcgatcc gcaatcgatc gtgctaaatg gcggagtatt
     1561 ggatctctct gatttcacca cctgggatgg cgatagctca tacaatgacg gtctgcaaat
     1621 cagcggcagc ggggggacag tgattggcag taatgatgtc gttgatatca gcagtggcga
     1681 tgatttacat attggcggca gtgatgccag ccagaatggc gtttatgtcg tcattaatgc
     1741 gggggatcag cgcgtaacgc tggcgaataa caacggttat ctcggtaata cgcaaatcgc
     1801 ctccggtacg cttgaagtca gcgataattc gcaattgggg gacacaagtt acaaccgttc
     1861 agtgatcttc acagatcccc aacaacacag tgaaatggat gtgacgactg atgttgacac
     1921 tcgctcggct acaacaggtc agggcaggaa tattgaaatg cgcgctgatg gcgaaataca
     1981 cgttgaggat ggcgtagata cgcaatgggg cgggttgatg gcggatagta ccgggcaaca
     2041 actggatagc gtaagcacgt tgactaaaag tggcggcggg acgctggagt tgaccgccag
     2101 cggcactgcg gcgtctgcgg tacgtgtgga agacggaacg ctaaaaggtg aagcggagaa
     2161 tattattcct tatgtttctt cactgtgggt gggggaagac ggtgtttttg aaaccgggca
     2221 aaatcaggat attcgctcaa tcgatgccac ctctggcggc gatatcgaca taaccgatgg
     2281 tactgtgttg cgattgacgc agcaggatac gaaccaggcg ctagatgcct cgctgtttag
     2341 cggcgacggt acgctggtga atgccaccga tggcgtgacg ttgacaggtg agcttaatac
     2401 caaccttgaa actgacagcc tgacttatct ttccgacgtg acggttaatg gcaatctgac
     2461 caatacgtcc ggtgctgtca gtctgcaaaa tggcatcgct ggcgacacgc tgacggtaaa
     2521 tggtgattat accggcggcg gtacgctact gctcgacagc gaattaaacg gcgatgactc
     2581 ggcaagcgac cagttggtgt tgaacggtaa tactgctggc aataccacgg ttgtgattaa
     2641 tcccattaca gggattggtg agccgacatc tacaggtatt aaagtggttg atttcgcagc
     2701 tgatcccacg cagtttcaaa acaatgcgca gttcagtctg gcaggaagcg gctacgtcaa
     2761 tatgggggcg tatgactaca cgctggtgga agataacaac gactggtatc tgcgatcaca
     2821 agaagtcaat ccgacgccgc cgcctgatcc agacccgact ccagatcctg atcccacgcc
     2881 ggatcccgac ccaacccccg accctgaacc tacgcctgct taccagccgg tgttgaatgc
     2941 caaagttggt ggatatttta ataacctacg tgcggcaaat caggcatttg tgatggagcg
     3001 acgcgatcac gcaggtggcg atggtcagac gctgaattta cgcgttatcg gcgggcgtta
     3061 tcattacacg gcggcagggc agttggctca gcatgaagac acttctacgg tgcagcttag
     3121 cggcaacctg tttagcgggc actggggtga tgatggcgag tggatgcttg ggattgttgg
     3181 tggctacagc gataaccagg gcgacagccg ctcgaatatg accggaactc gcgccgataa
     3241 ccagaaccgc ggttatgccg ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa
     3301 gcagggggcc tggttggata gctggctgca atatgcgtgg tttaacaatg atgtttccga
     3361 acaagacgat ggagtggatc attaccactc gtcggggatt atcgcctcgc tggaagccgg
     3421 ttatcagtgg ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca
     3481 gggcgtgcag caggatgatt ttaccgccgc taaccatgcg cgcgtgtcac aatcgcaggg
     3541 tgatgatatt cagatgcggt tgggtttaca cagcgaatgg cgtactgctg ttggtgtcac
     3601 gccaacgtta gatctgaatt attatcacga tccccatgcg acggaaattg aagaggatgg
     3661 cagcactatc agtgacgatg cggtgaagca acggggtgaa ataaaagtgg gaataacggg
     3721 caatatcagt cagcgagttt cgctgcgcgg cagcgtggcg tggcagaaag ggagtgatga
     3781 ttttgcccag atggcagggt ttttgtcgat gacagtgaaa tggtaatcgc tgtctgaccg
     3841 gcactgttgc cggatgcggc gtaaacgcct tatctggcct acggggagca tttgtaggcc
     3901 tgataagacg cggcaagcgt cgcatcaggc ttggcgcggt tgccggatga atccaaacaa
     3961 caacctcact tattctgcgt atgcagcata tagttcacat ccacgccggg gccgagctta
     4021 aaggtgttag tgatagggtt gtaatgcagc ccggtcatat gccgttcttt aagactggtc
     4081 tgatccaccc agcccagcaa ttctgccggt ttaataaact tcttcacatc atgtgtacct
     4141 ttaggcacca tgcgcaaaat atattcagca ccaaccaccg ccatcagcca tgacttgccg
     4201 ttgcggttaa gcgtcgagaa aaagacatcg ccgcccggtt tcaccagttg cgcacaggct
     4261 ctgaccactg actgaggatc ggggacgtgc tccagcatct ccatacaggt caccacgtca
     4321 tactgtccgg catgttttgc cgcgtgctct tccacggttt cctgcacgta atccacctga
     4381 atgccgcttt ccagtgcgtg cagttttgcc acctgcaatg gctcaaagcc catatccaga
     4441 ccggtcaccg tcgcgccctc ccgcgccata ctctcggcca gaatgccgcc gccacaaccg
     4501 acatcgagca cctttttgcc aaataaaccg ccagcacgct cggcaatata gcccagacgc
     4561 agcgggttaa tgcggtgcag cggtttgaac tcaccttcaa gatcccacca gcgggaggcg
     4621 acggcttcaa atttagcgat ctcttcgtgg tctacgttgt ggtttaccgg cgatttttcg
     4681 gcattcattg gcacttctac tccgtaattg gcaagacaaa cgagtatatc aggcattgga
     4741 tgtgaataaa gcgtataggt ttacctcaaa ctgcgcggct gtgttataat ttgcgacctt
     4801 tgaatccggg atacagtaga gggatagcgg ttagatgagc gaccttgcga gagaaattac
     4861 accggtcaac attgaggaag agctgaagag ctcctatctg gattatgcga tgtcggtcat
     4921 tgttggccgt gcgctgccag atgtccgaga tggcctgaag ccggtacacc gtcgcgtact
     4981 ttacgccatg aacgtactag gcaatgactg gaacaaagcc tataaaaaat ctgcccgtgt
     5041 cgttggtgac gtaatcggta aataccatcc ccatggtgac tcggcggttt atgacacgat
     5101 cgtccgtatg gcgcagccat tctcgctgcg ttacatgctg gtagacggtc agggtaactt
     5161 cggttccatc gacggcgact ctgcggcggc aatgcgttat acggaaatcc gtctggcgaa
     5221 aattgcccat gaactgatgg ccgatctcga aaaagagacg gtcgatttcg ttgataacta
     5281 tgacggcacg gaaaaaattc cggacgtcat gccaaccaaa attcctaacc tgctggtgaa
     5341 cggttcttcc ggtatcgccg taggtatggc aaccaacatt ccgccgcaca acctgacgga
     5401 agtcatcaac ggttgtctgg cgtatatcga tgatgaagac atcagcattg aagggctgat
     5461 ggaacacatc ccggggccgg acttcccgac ggcggcaatc attaacggtc gtcgcggtat
     5521 tgaagaagct taccgtaccg gtcgcggcaa ggtatatatc cgtgcccgtg ccgaagtaga
     5581 agttgacgcc aaaaccggac gtgaaaccat tatcgtccac gaaattccgt atcaggtgaa
     5641 caaagcgcgc ctgatcgaaa aaattgcgga actggtaaaa gaaaaacgcg tggaaggcat
     5701 cagcgcgctg cgtgacgagt ctgacaaaga cggtatgcgc atcgtgattg aagtcaaacg
     5761 cgatgcggtc ggtgaagtgg tgctcaacaa cctctactcc cagacccagt tgcaggtttc
     5821 tttcggtatt aacatggtgg cattgcacca tggtcagccg aagatcatga acctgaaaga
     5881 catcatcgcg gcgtttgttc gtcaccgccg tgaagtggtg acccgtcgta ccattttcga
     5941 actgcgtaaa gctcgcgatc gtgctcatat tcttgaagca ttagccgtgg cgctggcgaa
     6001 catcgacccg atcatcgaac tgatccgtca tgcgccgacg cctgcagaag cgaaaactgc
     6061 gctggttgct aatccgtggc agctgggcaa cgttgctgcg atgctggaac gtgcgggcga
     6121 tgatgctgcg cgtccggaat ggctggagcc agagttcggc gtgcgtgatg gtctgtacta
     6181 cctgaccgaa cagcaagctc aggcgattct ggatctgcgt ttgcagaaac tgaccggcct
     6241 tgagcacgaa aaactgctcg acgaatacaa agagctgctg gatcagattg cggagctgtt
     6301 gcgtattctg ggtagcgccg atcgtctgat ggaagtgatc cgtgaagagc tggagctggt
     6361 tcgtgaacag ttcggtgaca aacgtcgtac cgaaatcacc gctaacagcg ccgacatcaa
     6421 cctggaagat ctgatcacac aggaagatgt ggtggtcacg ctttctcacc agggttacgt
     6481 gaagtatcag ccgctttctg aatacgaagc gcagcgtcgt ggcgggaaag gtaaatctgc
     6541 cgcacgtatt aaagaagaag actttattga ccgactgctg gtggcgaaca cccacgacca
     6601 tattctgtgc ttctccagcc gtggccgcgt ctattcgatg aaggtctacc agttgccaga
     6661 agctactcgt ggcgcacgtg gtcgtccgat cgtcaacctg ctgccgctgg aacaggacga
     6721 acgtatcacc gcgatcctgc cggtgaccga gtttgaagaa ggcgtgaaag tcttcatggc
     6781 gaccgctaac ggtactgtga agaaaaccgt cctcaccgag ttcaaccgtc tgcgtaccgc
     6841 cggtaaagtg gcgatcaaac tggttgacgg cgatgagctg atcggcgttg acctgaccag
     6901 cggcgaagac gaagtaatgc tgttctccgc tgaaggtaaa gtggtgcgct ttaaagagtc
     6961 ttctgtccgt gcgatgggct gcaacaccac cggtgttcgt ggtattcgtt taggcgaagg
     7021 cgataaagtc gtctctctga tcgtgcctcg tggcgatggc gcaatcctca ccgcaacgca
     7081 gaacggttat ggtaaacgta ccgcagtggc ggaataccca accaagtcgc gtgcgacgaa
     7141 aggggttatc tccatcaagg ttaccgaacg taacggttta gtggttggcg cggtgcaggt
     7201 agatgattgc gaccagatca tgatgatcac cgacgccggt acgctggtac gtactcgcgt
     7261 gtcggaaatc agcatcgtag gccgtaacac ccagggcgta atccttatcc gtacttcgga
     7321 agatgaaaac gtggtgggtc tgcaacgtgt tgctgaaccg gttgacgagg aagatctgga
     7381 taccatcgac ggcagtgccg cggaagggga cgatgaaatc gctccggaag tggacgttga
     7441 cgacgagcca gaagaagaat aatttcatcg cttcatgcca aaagggagcc atctcccttt
     7501 tttgaattga aaagtccagg ctgtaaagtc tgggcttttg tcgtattagg gcgcggtaaa
     7561 gtttggctgt gcccgtaaaa aatggctggc tatacacaag gaatgtggca atgagtggtg
     7621 aaaaaaaggc gaaaggctgg cggttttatg gccttgttgg ttttggggca atagcgctgc
     7681 tatccgctgg cgtatgggca ttacaatact ctggcagtgg gccagaaaaa acgttgtcgc
     7741 cactggtggt gcacaacaat ctgcaaatcg atctcaatga gccggacctc tttctcgaca
     7801 gcgactctct gagccagctt cccaaagatc tccttaccat tccgtttctc cacgatgttc
     7861 tgagcgaaga tttcgttttc tattatcaga atcatgccga tcgtctgggc attgaaggca
     7921 gcattcgccg cattgtctat gaacacgatc tcacgctgaa agataagctc ttttcgtcac
     7981 tcttagacca gcccgcgcag gcggcactgt ggcacgataa acaaggccat ctttctcatt
     8041 atatggtgct gatccagcga agtggtttaa gcaagctgct ggagccattg ctgtttgccg
     8101 ctaccagcga cagccagtta agtaaaacgg aaatcagtag catcaagata aatagtgaaa
     8161 ctattcctgt ttatcagttg cgctataacg gcaataacgt cctgatgttt gcgacttatc
     8221 aggacaaaat attggctttt tccagttcgg atatgctgtt taaagatgac caacaggata
     8281 ccgaagccac ggcgatcgca agtgatttgt tgagtggtaa aaaacgctgg gaaacccgct
     8341 ttggcctgga agcgcgtact gccgaaaaaa cgccagtacg ccagcgcatc gtagtcagcg
     8401 ccaggttgct ggggtttggc taccagcgat taatgccttc ttttgctggc gtacgcttcg
     8461 aaatgggtaa cgacggctgg cacagttttc tggcgttaaa cgatgaatcc gccagcgtag
     8521 atgccagttt cgattttacg ccggtatgga acagtatgcc tgccggagcc agcttctgtg
     8581 tggcggtgcc gtattcacac ggtattgccg aagagatgct ttcgcacatc agccaggaaa
     8641 acgacaagct gaatggggcg ttagacggtg gcgcggggct gtgctggtat gaagactcaa
     8701 aattgcaaac cccactgttt gttggtcagt ttgatggcac tgccgaacag gcgcaattgc
     8761 cagggaaact gtttacgcaa aatattggtg cgcacgaaag caaagcgcca gaaggtgttt
     8821 tgccggtaag ccagactcag cagggcgaag cgcaaatctg gcgtcgcgaa gtgagttccc
     8881 gatacggtca gtatccgaaa gcgcaggcgg cgcaacccga tcaattaatg tcggattatt
     8941 ttttccgcgt gtcgctggcg atgcaaaaca aaacgctgct tttctccctc gatgacacgc
     9001 tggttaataa cgccctgcaa acactgaata aaacccgccc ggcaatggtg gatgtaatac
     9061 ccactgatgg catcgttccg ctctatatca atccacaagg aatggcgaaa ctgctgcgta
     9121 acgaaacgct gaccagtctg ccgaagaatc tcgaaccggt tttttataac gccgcacaaa
     9181 ctttattaat gccgaagctg gacgctttat ctcaacaacc gcgttatgtc atgaagctgg
     9241 cccagatgga acccggtgtc gcctggcagt ggctacccat aacctggcaa ccgctatgag
     9301 acacgggctg ctggcgctga tttgctggct gtattgtgtt gttgcccata gcgaaatgct
     9361 aaatgtcgaa caatccgggc tgtttcgcgc gtggtttgtg cgcattgcac aggaacagct
     9421 ccgccagggg ccaagcccac gctggtatca gcaggattgt gcgggcctgg tgcgatttgc
     9481 ggcgaacgag gcgctgaaag ttcacgacag taaatggcta aaaagtaacg gtttatctaa
     9541 ccagtatttg ccgccagaga tgacgctaac acctgaacag cgtcaactgg cgcaaaactg
     9601 gaatcagggg aatgggaaaa ccggccccta tgtgaccgcg attaatttga ttcagtacaa
     9661 cagccagttt attggccagg acataaacca ggcactgcct ggcgatatga tttttttcga
     9721 tcagggcgat gcccagcact taatggtctg gatggggcgt tacatcatct accacaccgg
     9781 aagcgccacg aaaactgaca acggaatgcg cgcagtcagt ctgcaacaac ttatgacatg
     9841 gaaggacacc cgatggatac ccaacgattc caatcccaat ttcattggca tttatcgttt
     9901 aaattttctg gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac agggcttgct
     9961 aatgctgagg attcgcttcc ttccagtaac tatgtgccgc ccgccggggg aacattcttt
    10021 ttgctcgctg acagcagttt tagcagcagt gaagaggcga aagtgcgact ggaagcgccg
    10081 gggcgtgatt atcggcgcta tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat
    10141 cgtattcctg acccgatggc atttttgcgc cagcagaaaa acctgcatcg cattgtggtg
    10201 caaccgcaat atctgggcga cggactgaac aatacgctga cctggctgtg ggataactgg
    10261 tacggcaaat ctcgccgcgt gatgcagcgt actttctctt ctcagtcacg gcagaatgtg
    10321 actcaggcgt tacccgaatt acagctcggt aatgccattg ttaaaccttc ccgttatgta
    10381 cagaacagcc agttttcacc gctgaaaaaa tatccacttg tggaacagtt ccgctatcca
    10441 ctatggcagg caaaaccggt cgagccgcag caaggggtaa aactggaagg cgcatccagc
    10501 aatttcatct cgccgcagcc gggcaacatc tatattcctc tcggcaaaca agagccggga
    10561 ctgtatctcg tcgaagcgat ggtgggtggg tatcgggcga cgacggtggt gtttgtttcc
    10621 gataccgtgg cgcttagcaa agtgtcaggt aacgagcttc tggtgtggac cgcggggaaa
    10681 aaacagggtg aagcgaagcc cggctcagag atcttgtgga ctgacggtct tggcgtgatg
    10741 acccgcggtg tgaccgatgg cagcggtacc ttgcagttac aacatatatc gccagaacgt
    10801 tcatacattc tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa ctttttctac
    10861 gaaagcgaaa tctacaacac ccgcttgtat atctttaccg atcgcccgct atatcgcgca
    10921 ggcgatcgtg tcgatgttaa agtgatgggt cgcgagttcc acgatccgtt gcattcatcc
    10981 cccatcgtca gcgcaccggc gaagctttcg gtgctggacg ccaacggcag tctgttgcaa
    11041 accgtcaatg tcacgctgga tgcgcgtaat ggcgggcagg ggagtttccg cctgccagaa
    11101 aatgccgtcg ccggaggtta tgagttacgt cttgcttacc gcaaacaggt ctatagcagc
    11161 agttttcgcg tggcaaacta catcaaaccc cattttgaga ttggtttagc tctcgacaaa
    11221 aaagagttca aaactggcga agcggtcagc ggcaaactgc aactgcttta cccggatggc
    11281 gagccggtaa aagatgcccg cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc
    11341 ggtaacgatt tgcgttatgc cggacgtttc ccggtgtcga tggaaggcag tgaaacggtt
    11401 tccgacgata atggtcatgt ggcgttgaat ctccccgccg ccgataaacc gagccgttat
    11461 ttgttaaccg tctccgccag tgacggcgcg gcgtatcgag ttaccaccac caaagagatc
    11521 ctcattgaac gtggcctggc gcattactct ttaagtaccg ccgcacaata cagtaatagc
    11581 ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt tcctgttacg
    11641 tatgaatggt tgcgtctcga agaccgcacg agtcacagcg gagagctaca gtcaggcggc
    11701 aaatccttta ccgtcaattt cgctaaacct ggcaactaca atctgacatt acgcgataaa
    11761 gacggcttaa ttctcgccgg gttaagccat gcagtcagcg gtaaggacag cacgtcgcat
    11821 agtggtacgg tagatatcgt ggcagataaa acactgtatc agcctggcga aaccgcgaag
    11881 atgctgatta ccttcccgga gccaattgac gaagcattat tgacgctgga acgcgatcgc
    11941 gtggaacagc agtcgctgct atcgcatccg gcaaactggc taacgctaca acgtttaaac
    12001 gatacccagt atgaagcccg cgtaccggtg agcaattcct ttgcgcctaa catcactttt
    12061 tcggtgctgt atacccgtaa cggtcagtac agttttcaga acgccgggat taaagttgcc
    12121 gttccccagc tggatatccg ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg
    12181 gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gttaacggta
    12241 ggcgtggtcg atgaaatgat ctacgcgctg caaccggaaa tcgcgccgaa tatcggcaaa
    12301 tttttctatc cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc
    12361 tacgatcagg cgctctccag cgagccggtt gcgcctggcg cgactaaccg cagcgagcgg
    12421 cgagtaaaaa tgctggaacg tccacggcgt gaagaggtgg ataccgcggc ctggatgccg
    12481 tcactcacaa ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta
    12541 acccgctggc gcatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt
    12601 gcttatttgc gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtatatcgc
    12661 atgggtgaca aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg
    12721 gcgctggtga ctaaatttgc aggtgctgag atgcgccaga cgctgacgct gcacaaaggg
    12781 gcgaattata tttcgctgac gcagaatatt cagcaatctg gcttgttaag tgcagaactg
    12841 caacaaaatg ggcaagtaca ggacagcatt agcacaaaac tgtcttttgt ggataacagg
    12901 tggcccgttg aacaacagaa aaatgtcatg ctcggcggtg gcgataatgc gctgatgttg
    12961 cccgagcagg cgagcaatat ccggctacaa agtagcgaaa cgccgcagga gattttccgt
    13021 aacaatcttg atgcgttagt cgatgaaccg tggggtggcg tgatcaacac cggtagccgt
    13081 ctgatcccgc tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac
    13141 gacattcgtc agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga
    13201 gcgcgcttta cctggtgggg tgaagatggc aatggcgacg ccttcctcac ggcatgggca
    13261 tggtacgccg actggcaggc cagccaggcg ctcggcgtaa cgcagcaacc ggaatactgg
    13321 caacatatgc tcgacagtta tgccgagcag gcggacaaca tgccgttatt gcatcgggcg
    13381 ctggtgctgg cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg
    13441 gatgaagcta tcgcccggcg cggaactaaa actgaagatt tctctgagga agacaccagc
    13501 gatatcaatg acagcatgat cctcgataca cctgaatctc cactggcaga tgcggtggca
    13561 aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga agtccactgt gatgccacag
    13621 gttcagcaat atgcgtggga taaagcagca aacagcaacc aaccgctggc gcacacggtt
    13681 gtgctgctca atagtggagg cgacgctacc caggcggccg ctattttaag tggtttaacc
    13741 gctgagcaat ccaccattga gcgcgcgttg gccatgaact ggctggcgaa atatatggcg
    13801 acgatgcctc cggttgtgtt acctgcgcct gcgggcgcat gggccaaaca taagttaacc
    13861 ggaggaggcg aatactggcg ttgggttggt cagggcgtgc cggacattct ctcttttggt
    13921 gacgaattac cgccgcaaaa cgtgcaggtc cgctggcgtg aaccggcaaa aacggctcaa
    13981 caaagtaata ttccggtgac cgttgaacgc cagttgtatc ggcttatccc cggtgaagaa
    14041 gagatgagct ttaccttaca gcctgtcacc agcaatgaga ttgacagcga tgcgctgtat
    14101 ctcgatgaaa tcacgcttac cagcgagcag gatgccgttc tgcgctatgg tcaggtggaa
    14161 gtaccgctgc cgccgggggc tgacgttgag cgtacaacct ggggcatttc ggtcaataaa
    14221 ccgaacgccg gaaaacagca aggacaactg ctggaaaaag cgcgcaatga aatgggtgaa
    14281 ctggcctata tggtgccggt aaaagaactg acgggaacgg tcaccttccg ccatttgctg
    14341 cgtttctcgc aaaaagggca attcgttctg ccgcctgccc gttatgtgcg ttcctatgca
    14401 cctgcacagc aaagtgttgc gccagggagc gaatggatcg ggatgcaggt gaaataagtg
    14461 aactggtaca gaatcgtctg gctattggcg ttggtaactc tgccaacgct tgcggaggag
    14521 acgccgttac agctggcgct gcgtggtgcg cagcacgatc aactgtatca gctgtcgtca
    14581 tcaggcgtga ccaaagtctc ggtattacct gacacgctca ccacgccatt agggagtttg
    14641 tggaaactgt atgtttacgc ctggctggag gatacccatc aaccggagca gccataccag
    14701 tgtcgcggca actccccgga agaggtttat tgttgtcagg ctggggagag cataacgcgc
    14761 gattccgcgc tggtacgttc ctgtggactc tattttgcgc cgcagcggtt gcatattggc
    14821 gcagatatgt gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctt
    14881 actacactga agccagaaac ctcggtaacg gttaaatcgc tgcttgattc attagcgacg
    14941 ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa
    15001 atcggtgtcg catccatgct cggtagtcgg gtacgggtga aaacctggag ctggtttgcc
    15061 gatgataaac aggaaatccg tcagggtggt tttgctggct ggttaaccga tggtacaccg
    15121 ctgtgggtta ctggcagtgg caccagcaaa acggtactga cccgttatgc gacggcgctt
    15181 aatcgggttt tgccagtgcc gacgcaagtt gccagtgggc aatgtgtgct ggtggatctg
    15241 tttgctcgtt atccgctgaa gaaagttact gaggaaaaga gtacgacggc agtcaaacta
    15301 ggcgtgctca atgggcgcta tcgtgtgacg tttgccaatg gtaaccacat gacatttgtc
    15361 agccacggtg agacaacgtt actgacggta aagggcaaac tgaaattaca gtcgcatctc
    15421 gatcgtgaag agtacgttgc cagagttctg gatcgcgagg cgaaaagtac gcctcctgag
    15481 gcagcgaaag ccatgaccgt ggcgattcgc acgtttttac agcaaaacgc cgatcgggaa
    15541 ggcgattgcc tgaccatccc ggacagcagc gctacgcagc gtgtttctgc ttcgccagct
    15601 accacggggg cgcgaacgat gaccgcctgg acgcaggatc tcatctatgc gggcgatccg
    15661 gttcattatc atggcagtcg cgctaccgaa gggacgcttt cctggcggca cgctactgcc
    15721 caggcggggc agggagagcg ttatgatcag atactcgcgt ttgcttatcc cgacaacagc
    15781 cttagccgct ggggagcgcc acgctcaacc tgccagttat tacccaaagc gaaagcctgg
    15841 ctggcgaaaa aaatgccgca gtggcggcgt atattacaag gtgagacggg atacaacgaa
    15901 ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc cctataccga tcggcagcag
    15961 aaacggctgt ttattcgtaa tttctttacg cttcaggatc ggctggattt aacccatgag
    16021 tatctgcacc tggcctttga tggttatccc accgggcttg atgagaacta tatcgaaacg
    16081 ctgacccgac aattattaat ggactaatgc tatgcgaaag atttttcttc cgttgttact
    16141 ggtggcgctt tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga ttgatgcacc
    16201 gctctctggc tggcatccgg cagagggcga agatgccagt ttttcacaaa cgatcaatta
    16261 cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga tccgcggcaa
    16321 aataaagaat tatgctgcgg cgggtaaagt tcagcagggc cggctggtgg tcaacggtgc
    16381 cagtatgccg cagagaattg aatcagatgg ttcttttgca cgcccttata ttttcactga
    16441 aggcagcaac agcgtgcagg tcatcagccc agatgggcaa agccgacaaa aaatgcagtt
    16501 ttactcaacg ccgggtgctg gggcgattcg cgcgcgttta cggctggttc tctcctggga
    16561 tacggacaat accgacctcg atcttcatgt cgttacgcct gacggtgaac atgcctggta
    16621 cggtaacaca gtgctgaaaa acagtggcgc actggatatg gatgtcacga cagggtacgg
    16681 acccgagatt ttcgccatgc cagcgccagt tcacggccgt tatcaggtgt atatcaacta
    16741 cttcggcggg cgcagtgaaa cggaattaac tacggcccaa ctgacgctga tcaccgatga
    16801 agggtcggtc aatgagaaac aggaaacttt tattgtaccg atgcgtaatg ctggcgagct
    16861 gacgctggtg aaaagttttg actggtgatt aatggggtaa ccttcgccgt cgatgttcaa
    16921 gaaaacgccc gataactttc gctatcgggc gtttttattg gttaattcaa ccgctcaatc
    16981 accatcgcaa ttccctggcc gccaccaatg catagtgttg ccagccccag cgttttatcg
    17041 cgtgcctgca ttgcatgtaa tagtgtgacc agaatacgag caccactggc accgatagga
    17101 tgtccgagcg cgatggcccc gccgttgaca ttcactttct caggatcaaa gcccagggtt
    17161 ttcccaacgg caaggaactg tgcagcaaat gcttcattag cctcaatgag atcaatatcc
    17221 gccagttgca gccccgccag ttgtaacgct ttttgcgtag caggtactgg ccccataccc
    17281 atcaatgcgg ggggcacgcc accgctggca taacttttaa tgcgtgccag gggattcagg
    17341 cctgctgcca gcgccgcaga ttcttccata atcaccagag cggcagcacc gtcgttaata
    17401 cctgacgcgt tcccggcggt gactgttcct gctttatcga aggccgggcg caatgcgcct
    17461 aacgcttcag ccgtagaatc cgctttcggg aattcgtctt gactgaagac gaaggttttc
    17521 ttccgggtga caacatttac cgggacgatt tcggctgtaa aagcaccgga ctcaattgcg
    17581 gctgccgctt tacgctgtga atgtagcgcc agttcatcct gcatttcacg ggtaattccg
    17641 tactctttag ccacgttttc ggcggtaatc cccatatgat aaccatgggt ggcgcacatc
    17701 aggccatcgc gcaggattac gtcataaacc tgtccgtctc caagacgata accagagcgt
    17761 gcttttgcat cgagtaagta gggcgctaaa ctcatatttt ccataccccc cgccacaatg
    17821 ctctgtgcct gacctgcctg aatcgcctgt gcagcaagcg ccacgctttt cagacctgaa
    17881 ccgcacactt tgttgaccgt gaatccgcac accgtttcag ctagtccgct ttttagcagc
    17941 gcctgacgtg ccggattttg ccccagtccg gcttgcaaca cgttacccat aatcacttca
    18001 tcaacgtgta gtgaatcgat ttttgcacgt tcaatggcgg ctttaattac tgtcgccccc
    18061 aggtcgatgg cgctggtgga agcgagtgaa ccgttaaaac taccgatagc agtacgtacc
    18121 gcactgacga tgacacaatt tttcatttta tattccttct gtctgtaggg ttgccgtcag
    18181 aacagcgtta aaccaatgac gaaaatgacg ccggagaaga gcagggcagt gatgcaatag
    18241 cccatgatgt cgcggacacc gagtccggcg attgccagtg ctggcagcgc ccagaacggt
    18301 tgtgccatgt tcatccattg ctcgccgtag gcgatcgcca ttaccgattt accgagatca
    18361 gcgcccagcg cctgggctgc gggcatcacg aacggcccct gaataaccca gtgcccgccg
    18421 ccagacggaa cggcgaagtt aatcagtgca gaactaaaaa aggtcatgac cgggaaggtg
    18481 tctttgttcg caacattgat gaagaattcg gtaatgagtc cgcccagacc ggagtgctcc
    18541 atcatcagtt ggatccccgc gtagaagggg aattgcacca gaataccggc ggtactgcgt
    18601 gctgccgcgc tgatagcacg catataagcc attggcgttt tatgtagcag cagacccgca
    18661 atcataaaca tcaggttgac ggtattgatg gtgatgttga agccatgttc gctgaagtac
    18721 atcgccaggt aagcgatacc gagtgcgccg atgatcaacg tcagaatgcg gctttcttcc
    18781 agtcgctcgg acggtggagc atctttcggt agttgctttt gaaaatcagc ctcttccatg
    18841 agtagtttcg gatcgatact caccacgtca gacggttttg gcatcatcat gcgggtgata
    18901 aatggcatca ccacaatcaa cgccacagtg atgaaaatat taaaaccact gaacagagta
    18961 tcgcccaccg ggatcagccc agcgatatgc tcaaccgggt tgcccggtgt tgcagccaac
    19021 agaggcattg agccagagaa gccgccaccc caggtgagaa aaccaatgta ggcgcaggca
    19081 atgagcaacg gataatcaga accgggtact cgccgggcga cttcacgggc gaacattgca
    19141 ccgacaacca aaccaaatcc ccagttgatg acacaagcga ctgaaccgaa gaaagtaacc
    19201 agcatgacgc cctgtacggg cgtctttgcg gcggaggcgg cagtacgcag caaacttttc
    19261 accggagcgg agctggcaag ggcatgaccg gtaacgatga taagcgccat ctgcatacca
    19321 aacgccagca agttccagaa accgtcaccc cacattttca ccatgctgat cggcgtttgt
    19381 ggtgttaacc aaagcgcgat cacgaatgtt agcaatgtca gcaacatggc aaagatcagt
    19441 ggatcgggaa gccaccggct gacaaaacgc gtcataaaac gcgatatgcg accaatcata
    19501 aatcaccccg ttgcgtattc agatcggcag ctacttcaaa ccgagcttct gttttggcac
    19561 gcacggtggc taaatcacac ccgtcggcaa tttcggtgag ccacattttg ccgtcaataa
    19621 aacgaaagac agccagttca gtaaccagca tatgcaccgc atgttgcgca gtgagtggca
    19681 tggtgcagcg gcgcaaaatt tttgctgaac catctttggc gcaatgttcc atggcgatga
    19741 tcactttgcg tgacccggtc accagatcca tcgcgccacc cataccgggc accattttcc
    19801 caggcactac ccagttcgcg aggtttgctt cttcgtctac ttgcaaaccg ccgagcacgc
    19861 aggcatcaat atgaccgcca cggattagcg caaatgacat ggcgctatca aacatggctg
    19921 caccgggtaa aatgccgcac ggttgtccgc cagcattcac cagatctgga tgcgctgttg
    19981 tgaccgggcc taaaccgagg aagccgtttt ccgattgcag agtgatatga ataccctccg
    20041 gtaaataatt ggcgaccatt gtgggtaaac cgatccctaa gttaacgatg tcaccatcac
    20101 gaagctcttg cgccacacgg cgcgcaatac gttgtttcgc atccattatt tgctctcctg
    20161 tgaaacgatg atgtggtcga taacggcacc aggggtgaca atatggtcag gttgcagctc
    20221 gccggtttcg accagttcat ccggctctac cagcgtgata tcagccgcaa gggctatcag
    20281 ggggttaaag ttgcgggcgc taaggtgata ggtcaggttg ccaagtgtgt cgcaacgatg
    20341 agcgcgaatt agcgccaggt cggcgcgcag cgggcgttcg agcagccagg ttttaccgtc
    20401 gagtgtcagt gtctgtttgc cttcctctac gacggtgccg acaccagttg gggtgagaaa
    20461 accaccaagt ccagctccgc cacagcgaat ttgctcgatt agcgtacctt gcggcaccag
    20521 aacgacgtcc atctcaccag atatcatgcg ccgacctgtt tccgggttgg tgccgatatg
    20581 tgaagcaatc actttgcgga ctcgaccatt gacgatgagc ggaccgatgc cggtatcaac
    20641 aaacgcggta tcattggcta tcaatgtcag gtcgcgaaca ccagattcca gtaatgcttc
    20701 aaccaggcgg gatggagtgc caatccccat aaatccgccc accatgatgg tcatgccgtc
    20761 acgaaagaag ccggtggcgt cttgtaatgt catcaatttt gttttcattt tttatccctc
    20821 ttgcatacac cgttgtgggt acagtcaggg aatagcaagg ggagtgccag aatgagatta
    20881 ttgaatgttg ttatttaact aattgaatag aaaaggataa attagatggg ggttcataag
    20941 gagaatgagc ggctatgcag aaaattgcgc actgtgcaaa tttctgcata gcaagttttg
    21001 gcattataca tccgccggat cgataccgta ttcctggagt ttatacatca atgcacggcg
    21061 actgatgccc agcattaaag cagtgcgggt tcggtttcct tcttgttgtt ccagcacttc
    21121 cataatgatg cgtttttcga cgcgtttaat ttcctctttt aaattacgct caccgacagg
    21181 ggctgttttt gcttcaccag cattacagac tggctgacga atctgcggcg gaagatcctc
    21241 agaaaaaatg atcgggcctg aattcatcac gacggcgcgt tcaataacgt tggaaagctc
    21301 tcgaatattt cccggccatg accaggcggt aagcagtgac attgccatcg gatcgatgtc
    21361 gataatatcg cgctgattct cactactgaa tttttgcaaa aagtgattag ctaacaggga
    21421 aatatcttcc cggcgatcgc gcagaggcgg cagtattaaa tgaataacgt taaggcgata
    21481 aaagagatct tcacggaagg tgccttcttt caccattgcc tgcaagtcgc ggttggtggc
    21541 agcaatgacg cggatatcaa cttttatggt ctgatggccg ccaatccgtt caaattcccg
    21601 ttcctgcaga atgcgtagta atttggcttg cagtaccagc ggcatttcgc caatttcgtc
    21661 gaggagcaga gtaccttcgt tggcacgttc aaataatccc tgacgcaagg tttgtgcacc
    21721 agtaaatgca cctttttcat gaccaaacag ttcactttcg agcaacgatt ccggcagtgc
    21781 cgcgcagttg actttaatga acggcccctt tgcccgccgc gaattgtagt gaatcgctct
    21841 ggcaatcaac tctttcccgg tgccgctttc accgctaatc aagacgctgg cctgagaaag
    21901 ggcaattttg gcggtgtctt tgcagatgtc catcatcgcc gggctgttgg tgagaatgtg
    21961 cccccattgc cagctggtgc tcagtgcctg gtgcagatga cggatctctt ttttcattga
    22021 ctggagttgt aaagcgcgct gaacgattaa attcaactca tcgagatcaa acggtttgat
    22081 aacatagtcg aaggctccgc agcgtagtgc ttcgacggcg gtttccactt cagcataggc
    22141 cgtcatcaga ataacgggtg tccgggtctc atggctgcgc atctccttta gtgccttgat
    22201 gccgtccatc tctggcatgc ggatatccat caacaccacg tcagggtgaa tatcggcaaa
    22261 caggtgtaat gctgtgcgcc cgttgttcgc acaatgtgtt tcgaatcctt gtagtgcaaa
    22321 agcggtgctc agcatacggc gaacattatc ttcatcatcc acaataagga tgcgattaat
    22381 agcagtcata cagtctgatt tccctgcggg ttgatcggta aaataagcgt gaaggttgcg
    22441 ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat gatgcgttga
    22501 cttaacgcca gaccaagccc ggttcctgag gctttggtag tgaaaaaggg atcgaagatc
    22561 tttttttgca gcgagagatc aatgccactg ccgttgtcct ctatcgaaat ggcctgttgc
    22621 gagtcgctat actgccaggt tcgaatgcga attttccctc gtgcactgat agcctggacg
    22681 gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc gttaatcggg
    22741 cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc ggcggtttgt
    22801 accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca ttgactgtgg
    22861 cgtgggcgtg aaaattcgag caattgctga ataactttgt taattgaatc gatttctttg
    22921 agtactacgg acagatattc ctgatgtatt gggtcacggg tttgttggcg caagatctgt
    22981 acataaccac gaatagccgt tagcggatta cgtacttcat gcgccacgcc agccatcagc
    23041 tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc tttgcgggca
    23101 gttaaatcag agaaaatcac caaagtacct atcatttcac cgtgcgtgtt atgaatacga
    23161 ctggtcgtga cactgagttc aatggtgcgg tcacggcctg gaaaactgat ctccagtgca
    23221 acatgttcgg tgccatgttc cagcgtatcc agtacgggac tgtagaactg agtattgtcg
    23281 aacaacatgg agtaaggctg ccctaccagt tcatgacgtt gatagccagt gatgacttct
    23341 gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac gccatcggca
    23401 gcgttttcaa taatcagatc gttaagtgtt cgcgtttcac gcagtgcctg ggcgaggttg
    23461 ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg agtgggaata
    23521 ttttgagcca gagtcgagag gccatcggtg atgatatcta tattggcgct caggcgacgg
    23581 gagaaaagga caatcaacag caggcttatc agcaaaccgg cggtgagcac aatgataatc
    23641 ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt ggcccagata
    23701 tagccgagga tttcaccatt acgctcaatg ggaatcattg aattcaaaat atcgccgcgc
    23761 acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg accagggtga
    23821 tctgcggcaa tggtaacgcc cacattattc tgatatagcg ctgaaggcgc gtaggtgatt
    23881 atcgcatcca gcgttttgtt gtaataacca gcgccgatgc cagggaaggc gtgagtgata
    23941 ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc acgcggtaag
    24001 tcgatgtaga gatcgtagcg attgcctagc gcctgattaa gcaggttgac cacggcagaa
    24061 agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac gatataacca
    24121 atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg attgcgtaag
    24181 cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct tgtccggtat
    24241 atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat tccagagaag
    24301 agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg
    24361 aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg
    24421 cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt
    24481 ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg
    24541 agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag
    24601 tacatagctg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa
    24661 acgcagacgg atgtgcctgc gtttgaaccg ctgtttgctg actctgattg ttccgcaatg
    24721 agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat
    24781 aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc
    24841 atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgct aaaagctttg
    24901 catgaacgca ttaataaata tcgaaatgca ccacaagatg atagtggcag taacctctac
    24961 tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgccttgac gccagtttat
    25021 ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaaa ccatccggat ggagaacttt
    25081 ttcttacctg gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg
    25141 atttcgctta ccggaccaga aagcaaaatt aagggcgatc ctcgctggat gcaggaacgc
    25201 tcctggtttg gctatacgga agggttccgg gaactggtgc tgaagaaaaa tctgccaccc
    25261 tcatcgctaa gcatcgttta ttcagtgcca gttgataagg tgctggaacg cattcgcatg
    25321 ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgcg
    25381 cggatgtacg agcggcgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa
    25441 catgagcagt ttaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt
    25501 accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacttatct caatatgctt
    25561 acgcatgagg accgccaacg gctgacacaa attatctgtg ggcagcaggt caattttgtt
    25621 gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt
    25681 aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag
    25741 tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctg
    25801 gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg
    25861 ttgcaaacca aagagttacc gaaaggcgtc gatcgtctgg tgacggcaat gaacaactct
    25921 tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaaaat tgaatcagaa
    25981 cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc
    26041 aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat
    26101 gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg
    26161 agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgttcg cgctgatggc
    26221 gattatctct ctatccgtgt tcgcgatacc ggcgtgggga taccggcgaa agaagtggtg
    26281 cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgtaactt ccaggggact
    26341 ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga catctcggta
    26401 gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttata cggcgctcag
    26461 tacccgcaga aaaaaggtgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc
    26521 aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat cgtcgttaca
    26581 acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgaac
    26641 aaaaaatggc agggcagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg
    26701 gagaaagcgc caggggagtg ggtacatagt gtggctgctc cgcatgagct accggcattg
    26761 ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg
    26821 acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg
    26881 attaaccggc gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg
    26941 aatgatggcg tcgatgcgct taatgtactt aacaagaatc atattgatat cgtgcttagc
    27001 gacgtcaata tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga
    27061 ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt
    27121 ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag
    27181 acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt
    27241 gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct
    27301 ggcctacttt aatgtaatct gttgaaataa tgggaatcgt aggacggata aggcgtttac
    27361 gccgcatccg gcaatccgtg caaatgccag ataagacact gacgcgtctt atctggccta
    27421 caggtgatta gtctttatct gccggactca acgtcacaga agagagataa ttcagcagag
    27481 caatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt
    27541 taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc gcaaacaggc
    27601 gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt
    27661 tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga
    27721 gagccttcgg caaatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat
    27781 ccaataccgc actaagaatc gccgggttgt tgttcatggt cagaacaatg atcgacaggc
    27841 ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcaccgtac ttatcgccag
    27901 gcatggagag atcggtaatc aacacatgcg catccagttt cggcagattg ttgatcagtg
    27961 ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc
    28021 gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt
    28081 aataggctac cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt
    28141 tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg
    28201 ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac
    28261 gcagcaaagt cgctggttgc tgcttcagta tatagcctct taacatcatc cggtactgtg
    28321 tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga
    28381 ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc
    28441 tgcatagcgt tgctcatatt gaaattgacg cacaattgac caggcccaat ttcccgtacg
    28501 ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt
    28561 aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc
    28621 caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt
    28681 acgcagacat catccagtaa tcgctcttct tcctcttcaa cttccgggtc agctgcgagc
    28741 attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga
    28801 ccgcccagtt tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca
    28861 tagcgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat ttcatgaatg
    28921 cttacgcctt ctccagtgtc cagaatgcgg aacgtcaggc ggtcttcgga ggactcatcc
    28981 tgatcaacct caagggtgat ttttcctaat tgcgttgagg tcacggcata ttgcatcagt
    29041 agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga
    29101 tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcact
    29161 acttcatcaa ttaaatcctg cacggagaac agctccgtct cacttttcca gctatcgtcc
    29221 gcaagcatgt tcgctaactg tatttcatcg accagtcgca ccagcacatc agcctgattc
    29281 gccagttgtt tgccttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg
    29341 ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc
    29401 tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg
    29461 cgaataatga aaatttgtgt gcgcggcgca acctggctgc ggaacatgcg gatctcatac
    29521 agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg
    29581 ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gactgtgcgg
    29641 tttgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta
    29701 attgcccgta aaatgcgcaa ttcgttattg accgccgtgc tggggacgct ttctgtactg
    29761 cggctggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg
    29821 ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcagtaaggt gccgtaagga
    29881 acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta
    29941 ctgttaaagt ggatactgac gctatccgtc ccttctttct cattttcatt gtttcccgtt
    30001 gccgtcgcgt ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca
    30061 ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt gaacgtggta
    30121 cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg
    30181 cgttcatcga gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg
    30241 ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc
    30301 accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc
    30361 cacaatgtgt ccagataggt ggacatccgt tgagtcatct caagcgttga gttgtcgtga
    30421 gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta gacatcctgt
    30481 ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc ggcaatgttg
    30541 tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat tgcgtgggta
    30601 atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct ttgtaccatt
    30661 acacccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc
    30721 ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac
    30781 ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact
    30841 cttacctgat tgatgattct ctattctcca gtgatattta tcatccggca ttgggggaaa
    30901 tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat
    30961 tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg aaatagttaa
    31021 caagcgttat agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa
    31081 aaaaaaccgg atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat
    31141 gacggtaata aataaagtta atgatgatag cgggagatat tctagttgcg agtgaaggtt
    31201 ttgttttgac attcagtgct gtc
//
LOCUS       DAJOTB010000042        30950 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJOTB010000042 DAJOTB010000000
VERSION     DAJOTB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.042"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..864
                     /gene="tuf"
                     /locus_tag="OHN28_001906"
     CDS             <1..864
                     /gene="tuf"
                     /locus_tag="OHN28_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2297309.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRT
                     VGAGVVAKVLS"
     gene            1094..1477
                     /gene="secE"
                     /locus_tag="OHN28_001907"
     CDS             1094..1477
                     /gene="secE"
                     /locus_tag="OHN28_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCQ2297310.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1479..2024
                     /gene="nusG"
                     /locus_tag="OHN28_001908"
     CDS             1479..2024
                     /gene="nusG"
                     /locus_tag="OHN28_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCQ2297311.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2183..2611
                     /gene="rplK"
                     /locus_tag="OHN28_001909"
     CDS             2183..2611
                     /gene="rplK"
                     /locus_tag="OHN28_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCQ2297312.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2615..3319
                     /gene="rplA"
                     /locus_tag="OHN28_001910"
     CDS             2615..3319
                     /gene="rplA"
                     /locus_tag="OHN28_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCQ2297313.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3611..4108
                     /gene="rplJ"
                     /locus_tag="OHN28_001911"
     CDS             3611..4108
                     /gene="rplJ"
                     /locus_tag="OHN28_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCQ2297314.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4175..4540
                     /gene="rplL"
                     /locus_tag="OHN28_001912"
     CDS             4175..4540
                     /gene="rplL"
                     /locus_tag="OHN28_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCQ2297315.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4860..8888
                     /gene="rpoB"
                     /locus_tag="OHN28_001913"
     CDS             4860..8888
                     /gene="rpoB"
                     /locus_tag="OHN28_001913"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCQ2297316.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLETLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8965..13188
                     /gene="rpoC"
                     /locus_tag="OHN28_001914"
     CDS             8965..13188
                     /gene="rpoC"
                     /locus_tag="OHN28_001914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCQ2297317.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="OHN28_001915"
     CDS             complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="OHN28_001915"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCQ2297318.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="OHN28_001916"
     CDS             complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="OHN28_001916"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCQ2297319.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="OHN28_001917"
     CDS             complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="OHN28_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCQ2297320.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="OHN28_001918"
     CDS             complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="OHN28_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCQ2297321.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="OHN28_001919"
     CDS             complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="OHN28_001919"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCQ2297322.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="OHN28_001920"
     CDS             complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="OHN28_001920"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCQ2297323.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GGLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18875..18975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="OHN28_001921"
     CDS             complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="OHN28_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCQ2297324.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            19598..20371
                     /gene="nudC"
                     /locus_tag="OHN28_001922"
     CDS             19598..20371
                     /gene="nudC"
                     /locus_tag="OHN28_001922"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCQ2297325.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20411..21475
                     /gene="hemE"
                     /locus_tag="OHN28_001923"
     CDS             20411..21475
                     /gene="hemE"
                     /locus_tag="OHN28_001923"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCQ2297326.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21485..22156
                     /gene="nfi"
                     /locus_tag="OHN28_001924"
     CDS             21485..22156
                     /gene="nfi"
                     /locus_tag="OHN28_001924"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCQ2297327.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22199..22789
                     /locus_tag="OHN28_001925"
     CDS             22199..22789
                     /locus_tag="OHN28_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCQ2297328.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22976..23248
                     /gene="hupA"
                     /locus_tag="OHN28_001926"
     CDS             22976..23248
                     /gene="hupA"
                     /locus_tag="OHN28_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCQ2297329.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23261..23956
                     /locus_tag="OHN28_001927"
     CDS             23261..23956
                     /locus_tag="OHN28_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCQ2297330.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="OHN28_001928"
     CDS             complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="OHN28_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCQ2297331.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            24615..25991
                     /gene="zraS"
                     /locus_tag="OHN28_001929"
     CDS             24615..25991
                     /gene="zraS"
                     /locus_tag="OHN28_001929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCQ2297332.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25988..27313
                     /gene="zraR"
                     /locus_tag="OHN28_001930"
     CDS             25988..27313
                     /gene="zraR"
                     /locus_tag="OHN28_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCQ2297333.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27310..28599)
                     /gene="purD"
                     /locus_tag="OHN28_001931"
     CDS             complement(27310..28599)
                     /gene="purD"
                     /locus_tag="OHN28_001931"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCQ2297334.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28611..30200)
                     /gene="purH"
                     /locus_tag="OHN28_001932"
     CDS             complement(28611..30200)
                     /gene="purH"
                     /locus_tag="OHN28_001932"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCQ2297335.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            30815..>30950
                     /locus_tag="OHN28_001933"
     rRNA            30815..>30950
                     /locus_tag="OHN28_001933"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7274 a         8006 c         8529 g         7141 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggt
      721 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      781 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      841 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      901 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      961 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
     1021 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
     1081 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
     1141 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
     1201 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
     1261 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
     1321 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
     1381 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
     1441 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
     1501 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
     1561 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
     1621 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
     1681 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1741 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1801 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1861 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1921 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1981 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     2041 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     2101 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     2161 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     2221 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     2281 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     2341 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     2401 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     2461 gaacaaagac aaagtgggta aaatttcccg cgctcaactg caggaaatcg cgcagaccaa
     2521 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     2581 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     2641 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     2701 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2761 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2821 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2881 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2941 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     3001 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     3061 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     3121 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     3181 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     3241 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     3301 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     3361 cacgtacgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     3421 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     3481 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     3541 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     3601 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     3661 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3721 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3781 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3841 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3901 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3961 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     4021 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     4081 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     4141 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     4201 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     4261 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     4321 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     4381 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     4441 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     4501 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     4561 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     4621 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     4681 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4741 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4801 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4861 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4921 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4981 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     5041 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     5101 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     5161 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     5221 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     5281 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     5341 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     5401 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     5461 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     5521 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     5581 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     5641 tgtacgtaga aaaaggccgc cgtattactg cgcgccacat tcgccagctg gaaaaagacg
     5701 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5761 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5821 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     5881 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5941 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     6001 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     6061 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     6121 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     6181 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     6241 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     6301 tgggcgatct ggaaaccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     6361 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     6421 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     6481 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     6541 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     6601 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     6661 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6721 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6781 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6841 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6901 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6961 gtacgggtat ggaacgtgct gttgccgttg actctggtgt aactgcggtt gctaaacgtg
     7021 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     7081 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     7141 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     7201 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     7261 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     7321 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     7381 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     7441 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     7501 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     7561 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     7621 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     7681 aagataaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7741 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7801 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7861 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7921 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7981 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     8041 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     8101 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     8161 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     8221 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     8281 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     8341 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     8401 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     8461 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     8521 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     8581 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     8641 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     8701 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8761 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8821 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8881 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8941 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     9001 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     9061 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     9121 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     9181 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     9241 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     9301 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     9361 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     9421 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     9481 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     9541 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     9601 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     9661 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9721 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9781 attaaccgta acaaccgtct gaaacgtctg ctggacctgg ctgcgccgga tatcatcgta
     9841 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9901 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9961 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
    10021 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
    10081 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
    10141 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
    10201 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
    10261 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
    10321 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggccgttca cgtaccgctg
    10381 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
    10441 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
    10501 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
    10561 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
    10621 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
    10681 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10741 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10801 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10861 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10921 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10981 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    11041 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    11101 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    11161 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    11221 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    11281 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    11341 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    11401 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    11461 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    11521 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    11581 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    11641 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caataaaggt
    11701 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11761 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11821 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11881 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11941 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    12001 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    12061 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    12121 ttgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    12181 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    12241 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    12301 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    12361 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    12421 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    12481 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    12541 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    12601 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    12661 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12721 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12781 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12841 gaagcgaacg gtaaagtggg tgcaacttac tcccgcgatc tgctgggtat taccaaagcg
    12901 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12961 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    13021 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    13081 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    13141 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    13201 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    13261 cttcacggga aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    13321 aacattcctg ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    13381 ttcatcttcc gtagcggctc ggaataaatt tacgttctta cattctgtct catggccttt
    13441 gcgatgggcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    13501 ttaacgcggc agcaaccgct tccggtctgc gatcgtcgtg cggtgagaac tgttccagct
    13561 cggggtggtt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    13621 ttgccagcgg aatcacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    13681 caatctccgg tgcaagcagc cggaaagcgc agatggcttg cactaactgg cgttcatcca
    13741 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13801 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttcggcaacc atatagcagt
    13861 caactcgcca gctgtcggaa aggccgatta gcgcgccgag gcctatctta tcaatccccg
    13921 cacgccctag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttacctttca
    13981 gatgatgatg ggcataagtc gcctcatgat atgtctcctg ataaaccatt actccatcga
    14041 ggccaagctg ttttaactcg gcatattccg cctccgccag cggttgcact tccatctgta
    14101 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    14161 tcgcctgatg ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg
    14221 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    14281 aaaatccgca ataagtacag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    14341 agctgacgac gttgccaaat cgctgacgag tcagacgttg cgctcgctgg gccagtggtt
    14401 ccagatagcc actggcggcg ggtgatagca atgccatcat gtcgtcgcgg gtgagttgcg
    14461 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggtggatgt
    14521 catcccagtc cagttgtcgc cagcgatcgc tgaaggtttt catgccgatg cctccagaaa
    14581 tccagtcagc gggctggtgg catgagcaaa atgactgcgg ctgcccggtc cggactgacg
    14641 tgccagcagg cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc
    14701 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14761 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaacggtgg cctgctggat
    14821 aataatctcc agcatggcgc gggtttccag tccctgattc gagccaatcg gcgcgccgag
    14881 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14941 gcagtaaggc agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    15001 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta gccagtttgt
    15061 gcctaacgct tcacgtgcca gatgggcggc gaaaatggct tcttccgcgg ttttcgcccc
    15121 ggaagtattc ggcagcaggg ccacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    15181 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agttggctgc cggaagcgcg
    15241 aatagactcc accatcagtt gtgaagaggc gaattttccg gtgccagtaa acagatgtga
    15301 atcaaacgtt ttgtccgcaa tatgtaacat ctcaaccccc tgcaataact tgaaaaagca
    15361 ggatctggtc gccatcctgc acgatatgtt gcgcccattg ttcccgcggg acgatttgct
    15421 gattaatcgc cagtgccgca cctgcttgtt gttgttcgag ttgctccagc agttcgtgaa
    15481 cagttagttc ggcgacacac tgcatcggtt gatcgttaaa caggatctgc attgcatcct
    15541 ccacataccg ggcaaccgct ggcgcggcgc aacgtcagac tgcgccactg gctcgattta
    15601 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cacttaataa cttaatcgct
    15661 tccagtgcct gtaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15721 cagttgcgtt ctggctcctg gttatctggc cacaggcagc ggtaacatcc ctgctcccag
    15781 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15841 gtgttgagtg ccacgcaggt ggcattaatc tcctggcgag tcgccatatt gtcagtacag
    15901 tcgagcacca catcggcttg tgcaaccgca tcttttaacg tctcacccgt taaccgttgt
    15961 tgtaatgcca tcagtcgaat atcgggattc aactgtgtca gtcgctgttg gctgacctgt
    16021 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    16081 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcccccgc caggtatagc
    16141 gcagcaggtg tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt
    16201 tgctgcccgt ccagagcgat atcgtcgagc aggatttggc ggctataacg cataaagtca
    16261 cggtcattca tcgccaactc ctgcaatttc cagcagctgt gccgttgcca aacgccagtc
    16321 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    16381 aggcgcgcgc gccagactga taccgccaat cgccacggtg ggataatccg ccaggcgctc
    16441 aacatgccgt gccagctgtt ccagtccctg cggtgcagaa ggcatctgtt tggtttgtgt
    16501 cggaaacaca tgtcccagcg cgatataaga aggacgtgcc gccagcgcga cgtcgatctc
    16561 catatcgtca tgagtcgaaa cgcccagccg caggcccgct gcacggatgg cattgagatc
    16621 ggtggcttgc aaatcttcct gccccagatg gacgccatac gcctgatgct taatcgccag
    16681 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16741 caccacatcg gcttctacct cttcatcgcg ctgatctttg atgcgtagct ggagagtacg
    16801 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tcaaccaccg ggtacagtcc
    16861 taaacgaaag ggtacaggag gaaactcagg ctgatacatc acgcttcctc cttacgcagg
    16921 tagatttctc cgcctctggc gcggaagttc tccgacatat ccgccattcc cacttcaatg
    16981 gtttgcgcgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggt
    17041 ccacacatgg agcaaaaatg ggcgacttta ccggactctt gcggcagggt ttcatcgtga
    17101 taagcgcggg cagtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    17161 aagcgggctt tcgacatggc gttatcgcga atttgcgcgc ccggatgccc tttcgccagg
    17221 tcggcggcgt gagcggcaat cttataggtg ataagcccct gtttaacatc ttctttattt
    17281 ggcaggccca gatgctcttt cggcgttacg tagcagagca tcgcgcagcc aaaccagcca
    17341 atcatcgccg cgccaatccc tgacgtgaag tggtcatagc ccggcgcgat atcggtagtt
    17401 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    17461 cggcgaatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacgtcatat
    17521 tcccaggcga ttttggtcag ttcgcccagc gtatgcagct cggcaaactg tgcttcatcg
    17581 ttggcgtcct gaatagaacc ggggcgcagg ccgtcgccca gcgacagcga aacgtcataa
    17641 gcagcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    17701 gagaggcacc atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc
    17761 gcggtcatcg gcacgtagcg cagcagcccc cccgcatgga tagtgaagta atccacacct
    17821 tgctcggctt gttccagcag ggtgtcgcgg aagacttccc aggtaagatc ttcagcgatc
    17881 ccgttcacct tctccagtgc ctggtagatc ggcactgtac cgatcggcac cgggctgtta
    17941 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccag tggaaagatc catcacggta
    18001 tccgctcccc agcgcgtgga ccataccagt ttttccactt cttcttcgat ggaagaagtg
    18061 accgccgagt tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc
    18121 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    18181 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    18241 tgctggtggc gtaaaacttc gctacgtatg cgctcgcgac ccatattctc gcgaatggcg
    18301 atgaattcca tttctggcgt gatgataccc ttgcgggcgt agtgcagttg ggtgacgcgg
    18361 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcaaggcca
    18421 tcatctgcca gccgcgcttt agtgtaatcg gaactgcgta cggtaagttc ttcggtatcg
    18481 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggca
    18541 atctgcggat cgccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    18601 tactgcggct gttctttgct gccgccaatt agcgtcgggc taagctggat ctcacgcatc
    18661 ggcacgcgca cgccggggtg tgtgccagtg atgtaaatgc gttttgagtt gggaaaggcg
    18721 gagccttcca gggtgtcgat aaaatgttgg gcctgggcgc gctgttcgcg gcgggtcagt
    18781 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18841 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18901 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18961 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    19021 cagaactcaa gcaggatatt tgacgcgggc ggcgtcaagc accagtagaa tcagcttatc
    19081 ttccagcaca aagcgcgctt ccagtgcctc gccgatgtca gataaaacct gttgaaactc
    19141 aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtcat ccataatctg
    19201 ttgagtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
    19261 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    19321 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    19381 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    19441 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    19501 catgattgac tccgcaagtt tgtattcaaa aactgctctg tgagaaatgt aaaaaccatg
    19561 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    19621 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    19681 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19741 atggcagggg gaacctgttt ggttagtaca acagcagcgg cgtcacgata tggggtcggt
    19801 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtgcaact
    19861 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19921 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19981 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    20041 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggtg aaaccctcga
    20101 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
    20161 cgtgacctcc caaccgtggc cgtttcctca gtcgttaatg accgcgttta tggcggaata
    20221 tgacagcggc gagatcgtga tcgacccgaa agaattgctt gaggcgaact ggtatcgcta
    20281 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    20341 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    20401 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    20461 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    20521 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaatgccg agctggcgtg
    20581 cgaagtgact ttgcaaccgc tgcgtcgcta tccgctggat gcggcgatcc tcttttccga
    20641 tatcctcacc gtgccagacg cgatgggttt agggctctat tttgaagccg gagaaggtcc
    20701 gcgttttacc tcgccagtca cctgcaaagc cgatgtcgat aaactgccaa ttccggaccc
    20761 ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20821 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20881 aggcggcagc agcaaagcgt tcaccgtgat caaaaaaatg atgtatgccg atccacaggc
    20941 gctgcacgcg ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat
    21001 taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    21061 cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt tactgcgtga
    21121 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggtggcggac agtggctgga
    21181 agcgatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    21241 tgcgcgccgt cgcgtgggca ataaagtcgc gctgcagggt aatatggacc cgtcgatgct
    21301 gtatgcgccg cctgcccgca ttgaagaaga agtggcgtct atacttgccg gtttcggtca
    21361 cggcgaaggc catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    21421 tgctggcgtg tttgtggagg cagtgcatcg cttgtctgaa cagtatcacc gctaaggagc
    21481 gattatggat ctcgcgtcat tacgcgctca acaaattgaa ctggcttctt ctgtgatccg
    21541 cgaggatcga ctcgataaag atccgccgga tctgatcgcc ggagccgatg tcggatttga
    21601 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    21661 ggtggagtat aaagtggccc gcatcgccac caccatgcct tacattccag gttttctctc
    21721 gttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt
    21781 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtag ccagccattt
    21841 tggcttattg gtggatgtgc cgaccattgg cgtggcgaaa aaacggctct gcggtaaatt
    21901 cgaaccgctc tccagcgaac cgggcgcgct ggcccctctg atggataaag gcgagcagct
    21961 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
    22021 ggtcagcgtg gacagcgcgc tggagtgggt acaacgctgc atgaaaggct atcgtctgcc
    22081 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    22141 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    22201 gttacaaaac ccaattcatc tgcgtctgga gcgcctggca agctggcagc atgtcacttt
    22261 tatggcctgc ttatgcgaac gcatgtaccc caattacgcc gtattctgtc agcaaaccgg
    22321 cttcggcgat gggcagattt accgccgcat tctcgatctc atttgggaaa cgctgaccgt
    22381 caaagatgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    22441 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcttgcg tggcgttaag
    22501 tgaactggtt cattcgcgtt taagtggcga aacgctcgaa cacgcggtgg aagtgagtaa
    22561 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    22621 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    22681 ccgactttta gctgagtgtg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22741 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22801 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22861 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22921 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22981 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    23041 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    23101 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    23161 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    23221 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    23281 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggcag gtgcgctgtt
    23341 tctcactgcc tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    23401 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    23461 cgtgtttagc ccgtggcgca gtggcgatac gacgacgcga gagtatcgct ggcagggcga
    23521 taacctcacg cttatcaata tcaacgttta cagcaaaccg ccggtaaaca tccgggcgcg
    23581 ttttgacgat cgcggtgact tgagctttat gcaacgtgaa tccgacgggc aaaagcagca
    23641 gctttccaat gatcagatcg atttataccg ttatcgtgcc gcgcagatcc gcgagattag
    23701 cgatgcctta cgccagggga gagtcgtgct gcgccagggg cgctggcatg cgatggagca
    23761 gactgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
    23821 gcatatcgag cgccgccaga gccgttcttc tgttgatgtc agcgtggcat ggctggaagc
    23881 gcccgaaggt tcgcaattac tgttggtggc aaactctgat ttctgtcgct ggcaacccaa
    23941 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    24001 ccgtaaccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    24061 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    24121 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg ctttgtcacc
    24181 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    24241 gtttgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    24301 gcaaatgccg atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    24361 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    24421 tcgtgccaac ggcgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    24481 gactcgcctg ttgtgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    24541 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgggc
    24601 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    24661 gcgcgatcct ccccgtggtc attgttgggc tggtggggct gtttgcggtg acagtgattc
    24721 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24781 ttatccgtgc tctcgaatcc ggctcgcgcg tcggaatggg gatgcgcatg catcatgcgc
    24841 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24901 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24961 attccccgca ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg
    25021 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    25081 ctggaatgca ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttta
    25141 tcgctttcga cgccagtaac attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    25201 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt gttgtcattc ttctggtatc
    25261 gccgatatct gcgctcgggc cagctgttgc aggatgaaat gaagcgcaaa gagaagctgg
    25321 tggcactggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga
    25381 ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    25441 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagctgctgg
    25501 aactggttaa gccaacgcat ctggctttgc aggcggtgga gctcaacacg ctgattagcc
    25561 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    25621 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    25681 atctctatct caacgctatt caggcgattg gtctgcatgg cgtgattagc gtgacggcca
    25741 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcgg
    25801 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggactgg
    25861 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gttgcaagcc
    25921 aggaggggaa aggcgcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    25981 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    26041 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    26101 gcgacaggcg ctggagcagg tgcgggaaca ggttttcgat cttgtgcttt gcgatgtgcg
    26161 aatggcagag atggacggca tcgccacgct aaaagagatc aaagcgttaa atccggcgat
    26221 tccggtactg attatgaccg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    26281 cggggcgctg gattacctca tcaagccgct ggatttcgat aacctgcagg caacgctgga
    26341 aaaggcgctc gcgcatacgc acagtatcga cgctgaaacg cctgcggtgt ctgccagcca
    26401 gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    26461 cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggtaccggta aagagctggt
    26521 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    26581 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcatgaaa aaggggcgtt
    26641 taccggagcc gacaaacggc gagaagggcg ctttgttgag gcggacggcg gcacgctgtt
    26701 tctcgatgag attggcgata tctcgccgat gatgcaggtt cgtctgctgc gtgcgattca
    26761 ggagcgcgaa gtgcaacgtg tcggcagcaa ccagactatc tcggttgatg tccggctgat
    26821 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26881 ctactatcgg ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    26941 tattcctctg ctggctggcc attttcttca gcgctttgcc gaacgtaatc gcaaggcggt
    27001 aaaaggcttt acgccccagg cgatggatct gctgattcat tacgactggc cgggaaatat
    27061 tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc
    27121 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggtac aaagtcagga
    27181 tattcagccg ctggtggaag tcgaaaaaga ggtgattctg gcggcgctgg agaaaacggg
    27241 cggcaacaaa accgaagccg cccgccagtt agggatcacg cgcaaaacgc tattggcaaa
    27301 actgtcgcgt tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa
    27361 gcagtcgtcc caatggatat cggtcattaa ggcataagcg cgtttctgcg cttctgccac
    27421 ggtatgaccc agcgcggtaa cgcacagtac gcgcccgccg ctggtcacta cctgctcgtc
    27481 atccgccagt ttcgtgcccg cgtggaacac tttgccgtct gccacttctt ccagcggcag
    27541 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    27601 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    27661 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27721 atcgccaaag cggcagttaa attcaataac ctttggatta ccctgtttgt cgatcatcag
    27781 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27841 ccagatgata cgttccatgg tgcgctgatg aacttcatcg gtcactaccg gcgcaggaga
    27901 gtaagccccc attccgccgg tgtttggccc ggtatcttta tcgcctacgc gtttgtgatc
    27961 ctggctggtg gccatcggta gcacatgctc gccgtccacc atcacgataa agctcgcttc
    28021 ttcaccgtcg aggaactctt cgataacaat acgatgaccc gcgtcgccaa acgcgttgcc
    28081 cgccagcata tcgtgaacag ctgcttcagc ttcttccagc gtcatcgcca cgataacgcc
    28141 tttcccggct gccagaccgt cagctttaat gacgattggc gcgcctttct cacgcagata
    28201 tgccagcgca ggttctacct cggtgaagtt ctggtattcc gctgtgggaa tgttatggcg
    28261 ggccagaaag tctttggtga atgctttaga accttccagt tgtgcagcac ctgcggttgg
    28321 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgact acgcctttca ccagcggcgc
    28381 ttccgggccg acgatggtta gatctacctt ttcgttttgt gcgaaatcca gcagcgccgg
    28441 gatatcggtt acgccgatag cgacgttctg tagcgcgggt tccagcgcag tgcctgcatt
    28501 gcccggagca acaaaaacag tttcaaccag cggcgactga gccgctttcc aggccagcgc
    28561 gtgctcgcgc ccgccgttac cgatcactaa tactttcatc tgtcgctcca ttaatggcgg
    28621 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaattact
    28681 tcgtcatcac ggatagaacc gccaggctgg attacgcagg tcacgcccgc agcggctgcg
    28741 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccattgacga acctttcact
    28801 tccaaacctt catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    28861 tggcctgcgc caataccgat ggtcatattg tttttggcat agacgatagc gttggatttc
    28921 acgaacttcg ccactttcca gcagaacagc gcatcacgaa gttcctgttc agtcggctga
    28981 cgtttggtga cgacgcgcag ttcttccgca ccgaccatcc ccagatcgcg atcctgaacc
    29041 agtagaccgc cgttcacgcg tttgaaatca agacccggaa tacgttcgcc ccactgaccg
    29101 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    29161 gacggcgcaa taatcacttc gacgaactga cgagaaatga tggcctgcgc ggtttcagca
    29221 tccagttcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    29281 gcgcgatcgt aagcatcaag aatggaattg ccgatagcca cgccgcaagg gttggcgtgc
    29341 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    29401 tcggcgatgt tgttataaga gagagcttta ccctgaacct gggttgcggt agcaacggag
    29461 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    29521 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct
    29581 tctttgcttt caccgtggta agccggaacc atgctgccga agtagttggc aatcatgctg
    29641 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    29701 agcgatcctt cgttggcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    29761 acgattgcga catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata
    29821 tcgatgttct ctaccgcatc ttccagcgag cagccttcac gagcgacggt ctgggcgaac
    29881 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29941 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    30001 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacat
    30061 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    30121 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    30181 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    30241 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    30301 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    30361 attttgacag tggctaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    30421 ctgtgtgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    30481 ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    30541 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc
    30601 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag
    30661 ccgcgtcgca actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac
    30721 ggattcttga cgtcgcaaga cgaaaaatga ataccaagtc tctgagtgaa cacgtaattc
    30781 attacgaagt ttaattcacg agcatcaaac ttttaaattg aagagtttga tcatggctca
    30841 gattgaacgc tggcggcagg cctaacacat gcaagtcgaa cggtaacaga aagcagcttg
    30901 ctgctttgct gacgagtggc ggacgggtga gtaatgtctg ggaaactgcc
//
LOCUS       DAJOTB010000043        30858 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJOTB010000043 DAJOTB010000000
VERSION     DAJOTB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.043"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..752
                     /gene="hofC"
                     /locus_tag="OHN28_001934"
     CDS             <1..752
                     /gene="hofC"
                     /locus_tag="OHN28_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCQ2297336.1"
                     /translation="QRQLTDKVKSALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTF
                     NTPLPALTQGIMTLASFSGEWGWLLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPI
                     MGSLMRGQKLTQIFTILTLTQSAGISFLQGVESVRETMRCPYWVQLLTQIQHDISNGH
                     PIWLALKNAGEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALADNLAALLEPA
                     LLIITGGIIGTLVVAMYLPIFHLGDAMSGMG"
     gene            complement(787..1830)
                     /gene="guaC"
                     /locus_tag="OHN28_001935"
     CDS             complement(787..1830)
                     /gene="guaC"
                     /locus_tag="OHN28_001935"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCQ2297337.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(1986..2030)
                     /locus_tag="OHN28_001936"
     CDS             complement(1986..2030)
                     /locus_tag="OHN28_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297338.1"
                     /translation="MIIKTLKMSARKRN"
     gene            2055..2675
                     /gene="coaE"
                     /locus_tag="OHN28_001937"
     CDS             2055..2675
                     /gene="coaE"
                     /locus_tag="OHN28_001937"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCQ2297339.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            2675..3418
                     /gene="zapD"
                     /locus_tag="OHN28_001938"
     CDS             2675..3418
                     /gene="zapD"
                     /locus_tag="OHN28_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCQ2297340.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            3428..3625
                     /gene="yacG"
                     /locus_tag="OHN28_001939"
     CDS             3428..3625
                     /gene="yacG"
                     /locus_tag="OHN28_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCQ2297341.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(3713..4111)
                     /gene="mutT"
                     /locus_tag="OHN28_001940"
     CDS             complement(3713..4111)
                     /gene="mutT"
                     /locus_tag="OHN28_001940"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCQ2297342.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(4171..6876)
                     /gene="secA"
                     /locus_tag="OHN28_001941"
     CDS             complement(4171..6876)
                     /gene="secA"
                     /locus_tag="OHN28_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCQ2297343.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(6938..7450)
                     /gene="secM"
                     /locus_tag="OHN28_001942"
     CDS             complement(6938..7450)
                     /gene="secM"
                     /locus_tag="OHN28_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCQ2297344.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(7680..8597)
                     /gene="lpxC"
                     /locus_tag="OHN28_001943"
     CDS             complement(7680..8597)
                     /gene="lpxC"
                     /locus_tag="OHN28_001943"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCQ2297345.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(8698..9849)
                     /gene="ftsZ"
                     /locus_tag="OHN28_001944"
     CDS             complement(8698..9849)
                     /gene="ftsZ"
                     /locus_tag="OHN28_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCQ2297346.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(9910..11172)
                     /gene="ftsA"
                     /locus_tag="OHN28_001945"
     CDS             complement(9910..11172)
                     /gene="ftsA"
                     /locus_tag="OHN28_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCQ2297347.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(11169..11999)
                     /gene="ftsQ"
                     /locus_tag="OHN28_001946"
     CDS             complement(11169..11999)
                     /gene="ftsQ"
                     /locus_tag="OHN28_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCQ2297348.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(12001..12921)
                     /gene="ddlB"
                     /locus_tag="OHN28_001947"
     CDS             complement(12001..12921)
                     /gene="ddlB"
                     /locus_tag="OHN28_001947"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2297349.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(12914..14389)
                     /gene="murC"
                     /locus_tag="OHN28_001948"
     CDS             complement(12914..14389)
                     /gene="murC"
                     /locus_tag="OHN28_001948"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCQ2297350.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(14443..15510)
                     /gene="murG"
                     /locus_tag="OHN28_001949"
     CDS             complement(14443..15510)
                     /gene="murG"
                     /locus_tag="OHN28_001949"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCQ2297351.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(15507..16751)
                     /gene="ftsW"
                     /locus_tag="OHN28_001950"
     CDS             complement(15507..16751)
                     /gene="ftsW"
                     /locus_tag="OHN28_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCQ2297352.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(16751..18067)
                     /gene="murD"
                     /locus_tag="OHN28_001951"
     CDS             complement(16751..18067)
                     /gene="murD"
                     /locus_tag="OHN28_001951"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCQ2297353.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(18070..19152)
                     /gene="mraY"
                     /locus_tag="OHN28_001952"
     CDS             complement(18070..19152)
                     /gene="mraY"
                     /locus_tag="OHN28_001952"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCQ2297354.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(19146..20504)
                     /gene="murF"
                     /locus_tag="OHN28_001953"
     CDS             complement(19146..20504)
                     /gene="murF"
                     /locus_tag="OHN28_001953"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCQ2297355.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDVIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(20501..21988)
                     /gene="murE"
                     /locus_tag="OHN28_001954"
     CDS             complement(20501..21988)
                     /gene="murE"
                     /locus_tag="OHN28_001954"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCQ2297356.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(21975..23741)
                     /gene="ftsI"
                     /locus_tag="OHN28_001955"
     CDS             complement(21975..23741)
                     /gene="ftsI"
                     /locus_tag="OHN28_001955"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCQ2297357.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(23757..24122)
                     /gene="ftsL"
                     /locus_tag="OHN28_001956"
     CDS             complement(23757..24122)
                     /gene="ftsL"
                     /locus_tag="OHN28_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCQ2297358.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(24119..25060)
                     /gene="rsmH"
                     /locus_tag="OHN28_001957"
     CDS             complement(24119..25060)
                     /gene="rsmH"
                     /locus_tag="OHN28_001957"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCQ2297359.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(25062..25520)
                     /gene="mraZ"
                     /locus_tag="OHN28_001958"
     CDS             complement(25062..25520)
                     /gene="mraZ"
                     /locus_tag="OHN28_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCQ2297360.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(26122..27126)
                     /gene="cra"
                     /locus_tag="OHN28_001959"
     CDS             complement(26122..27126)
                     /gene="cra"
                     /locus_tag="OHN28_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCQ2297361.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(27208..27294)
                     /locus_tag="OHN28_001960"
     CDS             complement(27208..27294)
                     /locus_tag="OHN28_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCQ2297362.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(27306..27797)
                     /gene="ilvN"
                     /locus_tag="OHN28_001961"
     CDS             complement(27306..27797)
                     /gene="ilvN"
                     /locus_tag="OHN28_001961"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2297363.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(27800..29524)
                     /gene="ilvI"
                     /locus_tag="OHN28_001962"
     CDS             complement(27800..29524)
                     /gene="ilvI"
                     /locus_tag="OHN28_001962"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCQ2297364.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGARFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            29643..29681
                     /locus_tag="OHN28_001963"
     CDS             29643..29681
                     /locus_tag="OHN28_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297365.1"
                     /translation="MKRNDKILHYRG"
     gene            complement(29842..30786)
                     /gene="leuO"
                     /locus_tag="OHN28_001964"
     CDS             complement(29842..30786)
                     /gene="leuO"
                     /locus_tag="OHN28_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCQ2297366.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7423 a         8683 c         7423 g         7329 t
ORIGIN      
        1 cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatt atcattttag
       61 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
      121 tctataagac ctttaacact ccgctaccgg cgctaacgca ggggatcatg acgctggcaa
      181 gttttagtgg cgaatggggt tggttgctgg tgctattcgg ctttctgctg gcgatagcca
      241 ataagctgct gatgcgccac ccaaccaggc ttatcgcacg gcagaaatta ctgttacgca
      301 tcccgattat gggttcactg atgcggggac aaaaactcac gcagatcttt acgattctga
      361 cgctgacaca aagtgcaggc atttcttttt tacagggcgt tgagagcgtc agagaaacaa
      421 tgcgctgtcc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
      481 accccatctg gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag
      541 tgagaacagg agaggcatcc ggctcgctgg atctcatgtt agacaacctc gcccatcatc
      601 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc
      661 tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc
      721 atttaggcga tgcgatgagt ggaatgggat aacgctggcg tgttgctcca cgccagcgtt
      781 ggtatattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga taaatgtggt
      841 gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
      901 gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt
      961 accttctgct gcgcgatatt ccgcaacacc gccaacgtga cgtttcatcg cagactctga
     1021 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
     1081 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
     1141 tttcgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc cgcccagacc
     1201 atgcgcagca tcggcacatt cgatcaccgc agaaagttgc ggataaccga cgcctgtttt
     1261 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
     1321 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
     1381 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
     1441 cacgtcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
     1501 gaaatccgca tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga
     1561 attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat
     1621 atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca tattggcggc
     1681 gataattggc acgccggacc agcgctgacc tgaatgtttg aaggtgaatt gacgttccag
     1741 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
     1801 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
     1861 ggtaagagcg caactccagt gacgttatca tacgcactaa tcaatgcggc gcaagactgc
     1921 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
     1981 tttcatcaat ttcgctttcg tgcgctcatt tttaacgttt tgataatcat gatgaattcc
     2041 cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga
     2101 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
     2161 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
     2221 ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
     2281 ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt cagcaagaga
     2341 cgcaacacca gatccagcaa gctacttccc cctatgtact atgggttgtg ccattgctgg
     2401 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
     2461 cgcaacttaa gcgcaccatg cagcgcgacg atgtaactcg cgagcatgtc gaacaaatcc
     2521 ttgctgctca ggcaacgcgc gaagctcgcc ttgccgtggc agatgacgtc attgataata
     2581 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
     2641 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
     2701 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
     2761 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagcga
     2821 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg
     2881 gcagcaacgt aaactccaga cctggattgg cgtgcccggc gtggaccaga gccgtattga
     2941 agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg
     3001 gcaatttcta cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccggg
     3061 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
     3121 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccacttaccc aggcacttac
     3181 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg
     3241 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
     3301 acagctttat ccgcaaattt ccggtcataa gagccgtttt gccattcgtt ttatgccgct
     3361 ggacagtgaa aacggacaag taccagaacg tctggatttc gaactggcct gttgctaagg
     3421 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
     3481 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
     3541 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
     3601 ctggagcgaa gagccaaagc agtgacattc tatctgatgc gatgctggcc tgataaaacg
     3661 cgccagcgtc gcatcaggca tcatatgcaa atgtcggatg cagcgtgacg ccttattcga
     3721 catacacacg cttaagtttc gcaattaccg gttcattggc tggcggaaaa tcatcggcat
     3781 taagaccgac cagcgacatc cactcaccgg gttgcccttc tttaccccac ggctcccctt
     3841 cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat tcatattcca
     3901 gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga agttcacgta
     3961 ccactgcctg ttccggcgtt tcgcccattt caattttacc gccggaaaac tccagtttat
     4021 tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat ttcattgttc tcgttgcgaa
     4081 taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa aggcgcagaa
     4141 tcctgcgcct tttacttcaa cagttagctt ttattgcagg cggccatggc actgcttgta
     4201 ttttttacca gaaccacacg ggcaaggatc gttacgtcct acttttcgtt caccggtttg
     4261 cgccgccagt gcagctgcgg ctgcagagtc gtcatcctga tggctaagct gctgcatttg
     4321 cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat
     4381 acgcacctga actttgctca gcgtgctgat aacttcatat tttaacgact ccagcatcgc
     4441 tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc
     4501 acgcaggtgg ataccctgac gcagatagtc catcgcagcc aggtgctctt tccacagaga
     4561 gtcaagcgtt tgcagcatga cgcctttctc gaagtgacgc atcatctcag caccaaccac
     4621 ttcttcttta cgctgataca cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc
     4681 ttcatgcagt tctggttctt tatccagcca ctcggcaatt ggcaaatcga ggtcgaaatc
     4741 gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagcg actgtggcgg
     4801 aatgtaggca tcaatggtcg ctttgaacac atcttcacga atgctgttga tggtttcgct
     4861 cacatcgctg acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt
     4921 agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacggc tttcaacttt
     4981 acgctgggcg ttggcaatcg ctttggtcac ccacgggtgc tcaatggctt cgcctggctt
     5041 catacctagt ttacgcatca tgccggatac tcggtcggag gcaaaaatac gcatcagcgc
     5101 atcttccatc gacagataga aacgggaaga accagcatcc ccctgacgac cagaacgacc
     5161 gcgcagctgg ttatcgatac gacgggattc gtgacgttca gtaccgatga tatgcaggcc
     5221 acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg
     5281 ctctgcagtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat
     5341 atcggtacca cgacccgcca tgttggtcgc gatagtcacc gcagccggat aacctgcctg
     5401 agcaacaatc gccgcttcgt tggcatggaa tttggcgttc aggacgttgt gcttaatacc
     5461 ggctttggtc agttcatttg acaccagctc cgatttttcg atggagattg tacccaccag
     5521 caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct gaattttttc
     5581 cgcttcagtc atgtagacca ggtccggcag atctttacga atcattggac ggttggtcgg
     5641 aacaacgaca gtatccagct tatagatgga gctgaattcg aaagcttcgg tatcagcagt
     5701 accagtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc
     5761 cagcgtttgg ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag
     5821 accatcagac cagcgacgcc cctgcatggt acgaccagtg tgttcgtcaa cgatgataac
     5881 ttcaccatct ttaacgatgt agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc
     5941 cgccgttacg tggtgcatca gcatgatgtt ggccggagag tacagagact ccccttcatc
     6001 catgatgcct tctttcacca gcagttcttc aatcagcacc agaccacgtt cggtcaggtt
     6061 cacctggcga gatttttcgt ccaccgagaa gtggccttcg ccctggaagg tttcggagtc
     6121 ttctttttcc tgacggatca ggtgcggaat aattttattc acgcgtttat acatttccga
     6181 gctgtcttct gccgggccag aaatgatcag cggtgtacgc gcttcatcga tcaggatgga
     6241 gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt caggactgaa
     6301 cgccatgttg tcacgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc
     6361 agcagcgtag gcttcacgct ttgccggtgc tggcatgccc ggcaggttga taccgacagt
     6421 caggccaagg aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc
     6481 gttgacggta acgacgtgca cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt
     6541 tgcggtcagg gttttacctt caccggtacg catttccgcg atgcagcgtt cattaagaac
     6601 cataccgccg agcagctgta cgtcgaagtg gcgcatgcca aaaacacgct tactggcttc
     6661 acgcaccacg gcgaaggctt ccgggatcaa gttttccagc acttcgcctt tttccagacg
     6721 cgcgcggaac tctgccgttt ttcctttcag ttcttcgtcg gagagttttt caatctccgg
     6781 ttccatggca ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg
     6841 actaccgaaa actttagtta ataatttgat tagcataata aaatctcaaa cgccccgcgt
     6901 tgcggagtta ataaaatgaa gtaaaggttt attgttgtta gctgaggcgt tgagggccag
     6961 cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgtggggtaa
     7021 aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcagcagcg
     7081 cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa
     7141 cgggcagcgt ttgcggtgcc attgcaaagg aaagatggcg gattaccgtg cgaatggcat
     7201 gttgatgcca gtaatcaacg gaatagttcg aattcgggcg gcgcgtgttc gtttccagca
     7261 aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg
     7321 cgggcgcgtt tggttcggcg gcattgctga gtgcaggcaa acctaaactc gccgcgacca
     7381 tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta
     7441 ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca caaatcttat
     7501 cattaaagcc tgaagtccac agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga
     7561 aaacagcgtt cgcaccgttt tttatagctc ttataaaatt caaaaaaaac gactggttca
     7621 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     7681 tatgccagta cggctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     7741 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     7801 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     7861 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     7921 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     7981 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     8041 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     8101 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggtttaaat
     8161 tcagcccact tatcgccatc ttcgacacga acagtctctt taatgcgaac aaatttcttg
     8221 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     8281 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     8341 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     8401 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     8461 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     8521 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     8581 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     8641 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     8701 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     8761 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     8821 accgtgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     8881 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acagttacgc gcagctcgtc
     8941 attcatatcc gggtcaagag aagtaccgat aaccacggtc gcgttgtcgg aagcaaatgc
     9001 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgctgtgat
     9061 gtttaccagc acgccgcgtg cgccagacag gtcgatatct tccagcagcg gagaagagat
     9121 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacaccag aacccatcat
     9181 tgcgtagccc atttcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     9241 cggacgagta atcagttcgg cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     9301 gccaaacgca tccagcaggg agataccacg gcccagaact ttcagcagtt tgtcgttcgg
     9361 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     9421 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     9481 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtgc caccacccat
     9541 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcagtg catcgcgatc
     9601 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     9661 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     9721 atttaccgcg aagaattcaa caccttcaat gcgctcacgc accatgtgtt caacagcatt
     9781 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     9841 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     9901 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     9961 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
    10021 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
    10081 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
    10141 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
    10201 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccagattgag
    10261 cagctcggta tagcgtggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
    10321 cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga tggagcccag
    10381 cgcacaaccg tggcgaactt taatcgcttc ggcgtcactt ggcggtgtgc caaaggcgta
    10441 agcgatatca ctggtcacga cattgccagc ataaggaatt accttcgtgt ggcgcaatgc
    10501 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
    10561 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagaccgg caaatatcag
    10621 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcatt
    10681 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
    10741 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
    10801 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
    10861 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
    10921 agaaagcgcc agatataccg aagatatctg acaatctgcc atcaattctg cctggtcaat
    10981 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
    11041 acgcgacggg caactaccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
    11101 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
    11161 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
    11221 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
    11281 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
    11341 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
    11401 tgccaggaac gtcgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
    11461 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
    11521 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
    11581 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
    11641 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
    11701 tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtgcctgg ctcacccaat
    11761 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
    11821 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
    11881 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
    11941 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
    12001 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
    12061 tgccgccatc ggcaccagac tgtggctggt catacccggt gaggtattgg cttccagcag
    12121 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
    12181 cgtcgtccat gcttttagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
    12241 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
    12301 tccggccggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
    12361 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
    12421 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
    12481 gcggctcggc ttaacgatta ccggcaaacc cagagaagaa atttctgcta actgcttatc
    12541 gttaaggcct ttttcaaact ctgcgcgggt taatgctacc cacggcgcga ccggtaaacc
    12601 ggcactctgc cacagaagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
    12661 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
    12721 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
    12781 cacgtcgact tctttcgggt cgacaggata cgcgtcaata ccgccttcac gcagcccggc
    12841 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaggtcc cgcccaacag
    12901 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
    12961 cagctaaaga acgagcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
    13021 taccggttaa taccggtgcc agcatctcgg ctacctgcgc cgggtccggc accagaatgg
    13081 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
    13141 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
    13201 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
    13261 ttaccaggtt tttatccggc cagcctgcac gcgccgcttt aatagtggcg tccacttccg
    13321 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
    13381 ccagcgggaa ttcaccgagg aaatcgaaac ggcgccccgt cccctggaag ctctccagcg
    13441 cacgcaaaat agcctcgtcg tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
    13501 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
    13561 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
    13621 cggcatcttc gctgaagccg taagtcgtgg tctgacgccc cacacgcggt aacaattcgc
    13681 ggatcaccgg atcatcaaca cacatcaccg cgcgaccgta aaacggcagg ttgtgcagaa
    13741 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    13801 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    13861 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    13921 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    13981 ccatcgcggt ggttgtcgtt ttgccgtgcg ttccggcaat ggcgatgcca tgacgaaaac
    14041 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    14101 cgacaatttc cgggttatcg gcagaaatcg cgctggaaac aacgaccacg ctggcatcac
    14161 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    14221 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    14281 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    14341 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    14401 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    14461 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    14521 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttcgcga cagcatccac
    14581 gctaagctgt gactgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    14641 ccagtattgc tgacggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    14701 ggcgatttca ctcaccgtta acgcaccgga gcggcaaacg acgacatccg cccacgcata
    14761 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccacttcggc
    14821 atacgcctgt tcaacggatt gttgcgaacc tttgccgctc tgatgccaga tagtgactga
    14881 atcacccagt ttcgcagcaa cctgcggcat tgtctgatta agaatgcgtg cgccctggga
    14941 accacccact accaacacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    15001 cggcagcgcc agcacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    15061 acctggaaac gcctgcatca ctttggtagc aatcttcgcc agccatttat tggttaagcc
    15121 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    15181 acctggacct gatacatagc cgcccatacc gagcaccacg tcaggtttgt acgctttcat
    15241 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    15301 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    15361 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    15421 cattagatgg tgcgcaaccg ccagccccgg gaatacatgt ccaccggttc cgcctgccat
    15481 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    15541 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    15601 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    15661 gccgccgcgc ctacgttaac cagcgcctga aaactaaacc agataccaat agaacaggcg
    15721 agaaaaccgg aaaaacggtg gtcaatttct aatgctttgc ggccaatcga catcgcgcga
    15781 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacatatcc cagttcttcg
    15841 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    15901 gagttaccta aaccttgtcc ccacagttca ccgcgaccaa acgccatcaa cgattgcgtt
    15961 aactgatagc cgctgccaaa gggatcttcc cacggattcc agaatgcggt aacacggcgg
    16021 atacggtacg gttcggcgag gatcagcaac acaaccgctg aaatgcccat accgataatg
    16081 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    16141 aacaccacca ccgtaccaag gtctggctgt gccagcagta acacagccaa caccagaatt
    16201 acgcccatcg gtttcagaaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    16261 agatagttgg cgatatagca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    16321 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagaacg
    16381 atcatcagca ggatgataga tccaagcagc atcgtggcac tgtagcgttg ccagaactcc
    16441 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    16501 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    16561 gtcaccatga taaagccaat cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg
    16621 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ctttagcgcc
    16681 gtggagatcc agaccagaat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    16741 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    16801 agttcttgaa ttgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    16861 gaacacgcgg agcaagcaag cgcatcgcct gttccatggt ttcggtttgt tctgccactt
    16921 ccgggcgtag cgccgccagc tgcgcgccgt cccgaccgaa acaatacaga cgtacattat
    16981 cgccattcag gtaacgcgcc agtgggctaa agtccgccga tttaccatcg ccacccagca
    17041 acaaatgcag cgtgccgtct acatgcaggc cattcagcgc cgcttccgta ctgccgacgt
    17101 tggtcgcttt tgaatcgtta acccagcgca caccgttatg ctccagcaca acttcaaagc
    17161 gatgcggcag accagtgaat gtggttaacg ctttcaggct gctggcacgc ggtaaccctg
    17221 cggcatctgc cagcgccagc gccgccagcg cattggtgta gttatgctgc ccggaaagtt
    17281 tcatttcttt cacgttcagc actttctcgc ctttcacccg cagccaggtt tcgccctgct
    17341 gatgattcag gtgatagtcg cccatgttga cgccaaagct gacgcagcgt tcatccgcac
    17401 cgcgaatcgg cattgttaag gcatcatcag cattaaccac gcaaactttt gcgttttcgt
    17461 aaatgcgcag ttttgctgca cgatactgtt gtaaaccaaa cggatagcga tccatatgat
    17521 cttcagtcac gttcagaatg gtcgccgcca ccgcttgtaa gctggaagtg gtttccagct
    17581 ggaaactcga cagttccagc acgtacagtt cgcactcatc atccaacagc atcaacgcag
    17641 gcaggccaat attaccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    17701 ctagcgtggt gaccgtgctt ttgccgttag aaccggtgat cgccacaatc ggtgcttgtg
    17761 cttcacgaca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    17821 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    17881 gccattcatc attcagaccg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    17941 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    18001 ccacgcagga aagcccggtg aggcccaggc cgataatgac gacattttta ccctggtaat
    18061 cagccatgat taacgtacct tcagcgttgc cagaccaatc aggaccagca tcagcgaaat
    18121 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    18181 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    18241 gacggaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    18301 cagcagtacg gcgataatac ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    18361 aaagacctgc gccggatagg tgttaaacca caggaagccc agccctgccc cgactatcgc
    18421 ggtacagaca atgaccagtt ccccggcgtg tcgtagatac ggtatgtgta aatagctggc
    18481 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    18541 cggcataatt gccagaccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    18601 aatgacaaag taagccagca gaatgtagaa cagccccagt tgcggcatca catctttaaa
    18661 gaacggaacc accaactgcg ttgcgggcgt gtctttgccg gcaaggtaca gcgcgaaggc
    18721 aacacccagt gcaatgaccg acatccagaa atacttccaa cgggcaatca ggcccttggt
    18781 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    18841 caccaccagt acgcaccaga cgtacggatt ggacgggtaa gcccacagca gcacggagat
    18901 cacaatcgcc gtcaggatca ttatcccgcc catggtcggt gtaccgcgct tgctgaagtg
    18961 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    19021 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    19081 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    19141 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta
    19201 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    19261 gcgataagcg cagttttatc ggaaaaatgt tcgccaacgc cgctggcggt gctgatagca
    19321 tggctttgtt tacccacact taacacgcag tcaataccag cagcttttgc cgcttcgccc
    19381 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    19441 acgcggtagc ccggcatttc agccagaacc tgaacagcgg cggtcattga accgacattg
    19501 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttttatggg gaacagacgg
    19561 cctggaacag ctttcagatt taccagcccc gctttgataa catcaagcgt tgcgcccaca
    19621 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    19681 agcagaacat cgacgctacc tgtaggggtt tgcagggtaa attccgtacc gtgcgaggtt
    19741 acatggatat tggtggcggt gaaatcgctg ttggcggcat taggtgagaa acgccacact
    19801 ttgcgtgagc caattacgct ctgccagttc aaccagtcgt tgttgtcggc gttcataatg
    19861 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    19921 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc cgcttccggg
    19981 cgagtcagac tcacagtcca ggcaatttca ccctgatggt tcgcgccaag ttcaataact
    20041 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttattg
    20101 agattgcctg ccgtataaag tgtgttgccg cactggctta aaattgccgc cgtcatctct
    20161 ttaacggagg ttttgccgga ggaacctgtc agagcaacca cgcgcgccgg aacttgctgg
    20221 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    20281 agatcaatat ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    20341 tcggcaaaat catgagcatc gaaacgttcg cctttcaggg caacaaacag gcagcccggc
    20401 gtcagttttc gcgtgtcagt ggttacagca tcaagggtaa tatctgcacc ttgcagttca
    20461 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    20521 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtagt
    20581 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    20641 aagtcaccgc ttcagcacgg ccttccatca ctttggcata tccggcatct aacattcccg
    20701 ccagaatatc gttgatgatg gcacgaggct cttcggtacg cgggttatcg tccgtcacca
    20761 ccaccacgtc agcaaactct tcagcaattg cgcccatcag tggacgtttg cctttatcac
    20821 gatcgccacc gcagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    20881 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    20941 ctggtgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca
    21001 gcaaatcagc cagcggatag ccgagtgcca gcagtgtcgc cagcgccagc agcaggttgc
    21061 tgacgttgaa tgcgcccatc aggcgacttt caatttcccc atcgccccaa ctcgagctaa
    21121 agcaaatggt cgcaccgctg tcgtgatagt tcacgtcggt cgctttcaac cagcgtccgt
    21181 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    21241 gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgtgcag
    21301 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    21361 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgttgaa
    21421 ccagcccgtg ggatgaaact tccattgcgc aaaaagtcgc gccctgatcc accagccccg
    21481 ccagctcatg ctgaacatcg actgccgaac cggttgtatt ttctgtcggg atcactttcc
    21541 ccagcaggcc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttgactcc
    21601 actgcgccaa cagctgggta gtcgtggttt tgccgttggt gcccgttacg cccacgagac
    21661 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    21721 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    21781 catctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    21841 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    21901 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    21961 gaaggtcgcg caaattacga tctgccacct gtaccctcgc cttgattaat cacaaattca
    22021 tttttatcgc ctgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    22081 atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga
    22141 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    22201 taagcaatat atttattgat gtagcgacca tccggcccga cctttttcgc ggtaccggtt
    22261 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc cggtagcgcc
    22321 acgctttcca tcatatgcac cacggtgcga acaatggatt ccgggaagac acgttcaccg
    22381 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    22441 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    22501 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    22561 accaacccca aattggtcgc ttttcccagc ccaaaacgtg agtaagtatc tactaacgct
    22621 gaggacggca tcgctaacgc cagtttggaa acaccgacgt tactcgactt ctgtaatacc
    22681 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    22741 taaggaacgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc
    22801 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    22861 atcgcttctt tcggcgtgcc gctcagatta tttgggttgt atgacgggct gttagccatc
    22921 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    22981 aaggccactg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    23041 agcgtcaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    23101 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    23161 ttctcgaccc cctcaatccc ttggctatcg acgttagtaa agccgatgag gtgagcagtc
    23221 acttcgccgg atggataata acggcgagat tcttcacgca gatgaattcc cggcagtttc
    23281 agttttttga tgtagtccgc catgtcaggg tttacctgac gcgccagata aataaagcgc
    23341 cctttcgggt tggcgttaat gcgggctgag agctgatcca gcggaatatt gagcgcgtta
    23401 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    23461 tcagcccaaa tcgcttttac tggcacactc accgctaacg ggcgaccaga acggtcagta
    23521 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    23581 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    23641 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    23701 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    23761 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    23821 gctatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    23881 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    23941 cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg
    24001 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacgccagg caaggcatgg
    24061 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    24121 atgcgttcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    24181 cctcttcttc gcccggcatc aacttaccta gtgctcgcaa ctgacgcccc cccagttttt
    24241 tgagctgctc ttctgtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    24301 gcataaaacg tttcacaatg cggtcttcaa gtgagtggaa gctgatgatc gaaagccgcc
    24361 cccccggggc cagcacattg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    24421 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    24481 tcaccggcgt tgcggcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    24541 cgcggttacg ctcgacaatg gcgcgagcaa tgcgtttggc aaaacgctct tcaccatagg
    24601 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    24661 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    24721 gttcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    24781 tgccgataag atcgcgctcg gcaacatatt cgcccagcgc agagaaaggt ccgtggatga
    24841 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    24901 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    24961 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    25021 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    25081 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    25141 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    25201 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    25261 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    25321 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    25381 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    25441 agcagctgtt cccgataacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    25501 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    25561 ccactaaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    25621 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    25681 cgaagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    25741 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    25801 ttttaggaca tatcttctct ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    25861 agcaggtttg cgcaaaaaca aagaaaagtg tttgttaaat cggcaattca tctctgcatg
    25921 taacaaaata atgcaaaaac gcgctgtcaa tatcatttaa ttattgtgca tttcagcagg
    25981 tgagttaatt aacgggcggg tttaaaagca tttatcaata atagccagga atagtcttat
    26041 ttactttaag gatattgatg tccattcccg tactcaacgc gccagagaga aattcacctg
    26101 gcgcgtactt ttgttcgcgg tttagctacg gctgagcacg ccgcggcgat agagattacg
    26161 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggctcg ccaggacaat
    26221 ctccagcaca cgttctgcga catcgcggtg acgttgagcc actgccagca ccgggcactg
    26281 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    26341 gccgtcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    26401 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagctgcctc
    26461 ccgctcatag ctgttggcat aaaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    26521 acggaagcct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    26581 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    26641 ggcaccaacc acgctggtga agtgttcacg atcgagggcg cggtccagcg cgacaatcgg
    26701 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    26761 aataatggca tcaacctgac gctgtaaaag gtgctcaata cagcgcattt cgttgtcggg
    26821 ctgatcttcc gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgttcaag
    26881 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    26941 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    27001 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    27061 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    27121 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    27181 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    27241 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcattgagat
    27301 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    27361 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    27421 cctgctaatt gaacggtata aagcgagggt gtgacatcga taatttgccc acggaatatt
    27481 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    27541 atttcccgct caacgtgcgc gccctgcccc aactcgctca cgcgcaagac atcgaccagc
    27601 ttgtgtaatt gcttttcgat ttgctcaagc actttttcat cgcccacggt ctggatggtc
    27661 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    27721 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    27781 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    27841 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    27901 acaccaggcg attattgcgc acctgttcca gcgcctcgct aagtttgctt tccagttctt
    27961 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaagtctg
    28021 gtaaggactg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgct
    28081 tcaccatccc cagatagcga ttattgagat tcaccaccag tacgggcaac tcgtattgca
    28141 aggcggtaga cagttcctgg atgttcatct gaatactgcc gtcaccagtg acgcaaacca
    28201 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    28261 tgccgaggcc gccagagttg atccagcgac gcggtttgtc gaatggataa taaagcgcag
    28321 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtccccatta gtcaaccgcc
    28381 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    28441 actgacgagc gcgccactgt tcaatctgct gccaccagtc gcggatctca tccagcggtt
    28501 gatgggcgga ttcttgcgac aggagttcaa gcatttgttc taggacctgg cgagcatccc
    28561 ccacaatcgg gatatcagca gtcacggttt tagaaattga ggttggatca atatcgatat
    28621 gcagaacagt ggcatttggg cagtattttg ccagattgtt cgtcgttcgg tcatcaaaac
    28681 gtgccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    28741 cgtgcattcc caacatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    28801 tcaaagagga aacaacgggc agattcaacg cctccaccgc ttctttcaac tgcagatggc
    28861 agcccgccgt gattgcccca ccgcctacgt agacaaccgg tttttttgcc gccaccagcg
    28921 tttgcagagc gcgcttaatt tgccctttat gcccggaggt cgtgggattg taagaacgca
    28981 tactgaccga ctccggccag acatagggta atttgttcgc cggatttaga atatctttcg
    29041 gtaaatcaac gaccacgggt ccaggacgac cacttgccgc tagccagaaa gcctttttca
    29101 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accgggcgag
    29161 aaatccccac catgtcgcac tcctgaaagg catcataacc tatcaacgaa gtcgctacct
    29221 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcaatg ccagtaatcg
    29281 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    29341 ccagaccatc ggccatatgc accgccgcct gctcatggcg aactaaaaca tgatcgatac
    29401 cacccacggt atgcaatgca tcgtaaatat caaggaccgc gcctccagga taaccgaata
    29461 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    29521 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    29581 tgcttaagca attggcagac agagacataa acacggggta ataagaataa acaggaggaa
    29641 aggtgaaaag aaacgataaa atcctccatt acagaggatg aaattgataa taaatcggct
    29701 gaatcctaca acttacagca atttctctat ttttttaatt aatcctaaaa tagagagcac
    29761 aaatattcag aataaaattc tacattcaat aaaaaatgca gaataaacca gacattgatg
    29821 tctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct cttccatcca
    29881 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctgtt
    29941 ttgttttaac ggcagcggta atacctgtaa ctctaaggat tcagcgaact cttcagccaa
    30001 ccaacgcggc gcaatagcaa ccagatgcgt ttgcgacacc acgctaagta cgctcatcat
    30061 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aggtttgact
    30121 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    30181 cagtaacggg cctttaattg ttggatgatt tttgctggct accaacacca tttcatcttt
    30241 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    30301 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    30361 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    30421 taacgggctg cagacacaaa gatgaaatac acgttcacta ctggcaggct caaagcctga
    30481 acctggtaat tcattttgta ctaattgcaa tgcctgacga actgagccaa aaagttgaaa
    30541 tgcgcgagca gtcggttgaa taccacgacc ataacgaaca aaaagctcgt cattaaacat
    30601 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacattccca gagcatgagc
    30661 ggcacgggtg atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    30721 gaccatgcgt agctgtggct tgcttaactc cgccgtctct ggatgatctg tttgtacctc
    30781 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    30841 taatcctcga gaatatga
//
LOCUS       DAJOTB010000044        30733 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJOTB010000044 DAJOTB010000000
VERSION     DAJOTB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.044"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..645
                     /locus_tag="OHN28_001965"
     CDS             <1..645
                     /locus_tag="OHN28_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2297367.1"
                     /translation="TDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEH
                     LGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFDP
                     YPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHN
                     SFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK"
     gene            complement(700..1074)
                     /locus_tag="OHN28_001966"
                     /pseudo
     CDS             complement(700..1074)
                     /locus_tag="OHN28_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1081..1682
                     /locus_tag="OHN28_001967"
                     /pseudo
     CDS             1081..1682
                     /locus_tag="OHN28_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1698..1774)
                     /locus_tag="OHN28_001968"
     tRNA            complement(1698..1774)
                     /locus_tag="OHN28_001968"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1738..1740),aa:Arg,seq:tct)
     gene            2045..2911
                     /gene="folD"
                     /locus_tag="OHN28_001969"
     CDS             2045..2911
                     /gene="folD"
                     /locus_tag="OHN28_001969"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCQ2297368.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            2913..3125
                     /gene="ybcJ"
                     /locus_tag="OHN28_001970"
     CDS             2913..3125
                     /gene="ybcJ"
                     /locus_tag="OHN28_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCQ2297369.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3233..3754
                     /locus_tag="OHN28_001971"
     CDS             3233..3754
                     /locus_tag="OHN28_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2297370.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(3790..5175)
                     /gene="cysS"
                     /locus_tag="OHN28_001972"
     CDS             complement(3790..5175)
                     /gene="cysS"
                     /locus_tag="OHN28_001972"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCQ2297371.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5349..5843
                     /gene="ppiB"
                     /locus_tag="OHN28_001973"
     CDS             5349..5843
                     /gene="ppiB"
                     /locus_tag="OHN28_001973"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCQ2297372.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            5846..6568
                     /gene="lpxH"
                     /locus_tag="OHN28_001974"
     CDS             5846..6568
                     /gene="lpxH"
                     /locus_tag="OHN28_001974"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCQ2297373.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6679..7008
                     /locus_tag="OHN28_001975"
     CDS             6679..7008
                     /locus_tag="OHN28_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297374.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            7104..7613
                     /gene="purE"
                     /locus_tag="OHN28_001976"
     CDS             7104..7613
                     /gene="purE"
                     /locus_tag="OHN28_001976"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCQ2297375.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            7610..8677
                     /gene="purK"
                     /locus_tag="OHN28_001977"
     CDS             7610..8677
                     /gene="purK"
                     /locus_tag="OHN28_001977"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCQ2297376.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(9211..10104)
                     /locus_tag="OHN28_001978"
     CDS             complement(9211..10104)
                     /locus_tag="OHN28_001978"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2297377.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(10101..10916)
                     /locus_tag="OHN28_001979"
     CDS             complement(10101..10916)
                     /locus_tag="OHN28_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2297378.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(10927..12186)
                     /locus_tag="OHN28_001980"
     CDS             complement(10927..12186)
                     /locus_tag="OHN28_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2297379.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(12196..13863)
                     /locus_tag="OHN28_001981"
     CDS             complement(12196..13863)
                     /locus_tag="OHN28_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2297380.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEKALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            14180..15229
                     /gene="allD"
                     /locus_tag="OHN28_001982"
     CDS             14180..15229
                     /gene="allD"
                     /locus_tag="OHN28_001982"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCQ2297381.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            15251..16486
                     /gene="allC"
                     /locus_tag="OHN28_001983"
     CDS             15251..16486
                     /gene="allC"
                     /locus_tag="OHN28_001983"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCQ2297382.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            16497..17282
                     /gene="allE"
                     /locus_tag="OHN28_001984"
     CDS             16497..17282
                     /gene="allE"
                     /locus_tag="OHN28_001984"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCQ2297383.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVYGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(17411..18556)
                     /gene="glxK"
                     /locus_tag="OHN28_001985"
     CDS             complement(17411..18556)
                     /gene="glxK"
                     /locus_tag="OHN28_001985"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCQ2297384.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(18578..19879)
                     /locus_tag="OHN28_001986"
     CDS             complement(18578..19879)
                     /locus_tag="OHN28_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCQ2297385.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(19935..21296)
                     /gene="allB"
                     /locus_tag="OHN28_001987"
     CDS             complement(19935..21296)
                     /gene="allB"
                     /locus_tag="OHN28_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HCQ2297386.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(21356..22810)
                     /locus_tag="OHN28_001988"
     CDS             complement(21356..22810)
                     /locus_tag="OHN28_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCQ2297387.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(22980..23858)
                     /gene="glxR"
                     /locus_tag="OHN28_001989"
     CDS             complement(22980..23858)
                     /gene="glxR"
                     /locus_tag="OHN28_001989"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2297388.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(23958..24734)
                     /gene="hyi"
                     /locus_tag="OHN28_001990"
     CDS             complement(23958..24734)
                     /gene="hyi"
                     /locus_tag="OHN28_001990"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCQ2297389.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(24747..26528)
                     /gene="gcl"
                     /locus_tag="OHN28_001991"
     CDS             complement(24747..26528)
                     /gene="gcl"
                     /locus_tag="OHN28_001991"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCQ2297390.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(26618..27433)
                     /gene="allR"
                     /locus_tag="OHN28_001992"
     CDS             complement(26618..27433)
                     /gene="allR"
                     /locus_tag="OHN28_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCQ2297391.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(27511..27993)
                     /gene="allA"
                     /locus_tag="OHN28_001993"
     CDS             complement(27511..27993)
                     /gene="allA"
                     /locus_tag="OHN28_001993"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCQ2297392.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            28223..29149
                     /gene="allS"
                     /locus_tag="OHN28_001994"
     CDS             28223..29149
                     /gene="allS"
                     /locus_tag="OHN28_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCQ2297393.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            29218..30324
                     /gene="mnmH"
                     /locus_tag="OHN28_001995"
     CDS             29218..30324
                     /gene="mnmH"
                     /locus_tag="OHN28_001995"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCQ2297394.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
     gene            complement(30376..>30733)
                     /locus_tag="OHN28_001996"
     CDS             complement(30376..>30733)
                     /locus_tag="OHN28_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCQ2297395.1"
                     /translation="HFCWDSVIDKKEYETWITLAGDVWKLMLTPYSPPGGGSEKYYLR
                     YLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAYKHGYSYPE
                     STKNFIKDKKYPYGNW"
BASE COUNT         7634 a         8264 c         7608 g         7227 t
ORIGIN      
        1 acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt
       61 ttgggtgtat caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac
      121 tttctggaac atttaggtaa caaccatctg gatattggcg ttggaactgg gttttacctt
      181 actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac
      241 gcggcatcga caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt
      301 gatccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt
      361 cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc
      421 ttaactgacg atggaactct atacggagcc acaattcttg gtgacggcgt tgtgcacaat
      481 agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa
      541 gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag
      601 gttaaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc agacgcagca
      661 cgttcttgcg cacgacgtgc tgcggcataa tcccaatgat tactctctga cagggttcac
      721 aggccactca atatcagggg cagttgatgt atcaactcgg ttcagcaaca cccgatacat
      781 cttccaggct tccagcaacg aggtttcttc ctccgtatcc tttatccatg atgtgccgtt
      841 ccacttctga aactcccctg ccggggataa ccaggtgaca ttttccggta acggaccgag
      901 ttcagaaata aataacgcgt cccctgatgc cacgtcataa accgttttac ccagatggtc
      961 ttcaacgaga tgccacgatg cctcatcact gttgaaaaca accacgaagc cagccggaat
     1021 atctggcggt gcaatatcgg tactgtttgc aggcaaacct gtatgaggtg gaatggcaca
     1081 agagacttac aaaagaatca ggcaaaacgc ctgattgatg agagccccga actactgaaa
     1141 tctgtcgtca aatttgcgct ggtaactgtt ctgagaaagt cgaacatcat aaatctggag
     1201 tggcaacaga ttgacatgca gcgacaagtt gccagggtgg atccagaaga tagcaaatca
     1261 aatcgcgcca ttggtgtggc gctgaacgat accgcctcta aagtgttgcg tgatcaaaca
     1321 ggcaagcatc acaaatgtgg gtgtacatac cagggcggct aagcgagcag atggaacatc
     1381 aatgcctgcg ctcaggaaga tgcgcattga cagcaagaca gcatggctat cagcttgtcg
     1441 tcgtgcagga gttgaaaatt tccgtttcca tgacctctgg catacccggg caagcaggtt
     1501 gattccgtca ggcgtcccat tatcagtgct tcaggaaatg ggcggatggg agtccagaga
     1561 aatggttcgt agatatgctc accttgcgcc taatcatttg actgagcacg cgaggaaaat
     1621 agacgacatt tttggtgata atgtcccact ctggaattat agaagaaata aagaaggcgt
     1681 aactgattga attttaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga
     1741 aggtcgttgc tctatccaac tgagctaagg gcgcattgat accacggtgt gctgtaatcg
     1801 cgtgaattat acggtcaacc cttgctgagt caatggcttt tgaaccagtt gctgaacaag
     1861 tgtacgaccg catcagatgt agctcactat ggcggcaaac caacgacggt tatcatgata
     1921 ggtgtgaatc agccaggtac agaaggtttt acttttcaga aaacgaaaac tgacagcgcg
     1981 ccacgcttct gacaaaatag gcgcatcccc ttcgacctac gtaacagatg gaatcctctc
     2041 tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc gctctgaagt
     2101 tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcgccaggac tggccgttgt
     2161 gctggtgggt agcaaccctg catcgcaaat ttatgtcgca agcaaacgca aggcttgtga
     2221 agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg aagcggagct
     2281 gctggagctt atcgatgcgc tgaatgccga caacaccatt gatggcattc tggttcaact
     2341 gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcatc cggacaaaga
     2401 cgtggacggt ttccatcctt acaacgtcgg tcgtctgtgc cagcgcgcgc cacgtctgcg
     2461 tccctgcacc ccgcgcggta tcgtcacgct gcttgagcgt tacaacattg ataccttcgg
     2521 ccttaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgcccaatga gcatggaact
     2581 gctgctggca ggttgcacca ccaccgtgac tcaccgtttc actaaaaatc tgcgtcacca
     2641 cgtagaaaat gccgatttgt tgatcgttgc cgtcggcaag ccgggcttta ttcctggtga
     2701 ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg aaaatggcaa
     2761 agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca ttacgcctgt
     2821 tccgggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc tacaggcgtg
     2881 cgttgaatat catgatccac agggtgagta acatggcgac attttcttta ggtaaacatc
     2941 cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc ggcgcgcagg
     3001 cgaaaatcgc gatagccgaa ggcctggtga aagtcgacgg tgcggttgaa acgcgcaaac
     3061 gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta caggttgttg
     3121 cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact tttgccctct
     3181 acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca tcatgccaac
     3241 cgttattacg cacgccgccg ttcccctatg cattggttta gggttgggtt cgaaagccat
     3301 ccctccgcgt ctgttatttg ccggaatcat cctggcgatg ctgcccgatg ccgatgtatt
     3361 gtcgtttaaa tttggcgttg cttacggaaa tgtttttggt catcgcgggt ttacccattc
     3421 gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg attggacgtc gatggttcag
     3481 ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc acagcttgct
     3541 ggattcggta accactggcg gtaaatgggt tggctggctg tggccgtggt cagatgaacg
     3601 ctttttcgct ccctggcagg tgattaaagt cgcgccgttt gcattgtctc gttacaccac
     3661 gccgtacggg catcaggtga ttatttctga attgatgtgg gtgtggttgc cggggatgtt
     3721 actgatggga atgttgtggt ggcgcagacg ataaccggat gcgaaaactc gcatccggca
     3781 atagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca gcacgatccc
     3841 catctcgtta agacggtcac gcgccgcatc tgccgccgcc cagtctttcg ctttacgggc
     3901 atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt ctgcctgcgc
     3961 accgctttgc aggaacgctt ccggttcttg ctccagcagg cccaatacgg cagaaagttt
     4021 acgcaggtga gacgccattg cattcgccgc tgccatatct tctgctttca gacggttcac
     4081 ttcacgcgcc atatcaaaca gtacggaata ggcttccgga gtgttgaaat cgtcgttcat
     4141 cgcctcgata aagcgcgctt caaacgcttc gccaccggca ggcgcaacgg ttttgtctgt
     4201 gccgcgcagc gcagtgtaga gacgttccag cgccgcacgc gcctgtttca ggttctcttc
     4261 gctgtagttc aactgactgc gatagtgacc cgacatcagg aagtaacgca cagtttccgc
     4321 atcgtagtat ttcagcacat cacgcacggt gaagaagtta cccagcgatt tagacatctt
     4381 ctcgcggtca accatcacca taccggagtg catccagtaa ttcacatact ggccatcgtg
     4441 cgcacaggtg gactgggcaa tttcgttctc gtggtgcggg aacatcaggt cagaaccgcc
     4501 gccgtggata tcaaagtggt tacccagctg cttgcagttc attgccgaac attcaatgtg
     4561 ccagccagga cggcccgcgc cccacggaga cggccagcta ggttcgccct ctttcgacat
     4621 tttccacaga acgaagtcca tcgggttgcg tttgtcgtcg accacgtcaa cgcgcgcgcc
     4681 tgcctgcagc tgatcgagat cctgacgcga cagcacgcca tatgtcggat cggtcgggac
     4741 gtcgaacatc acgtcgccgt tgtcagccac ataagcgtga cctttagcga tcagttgttc
     4801 agtgagttca ataatttcgg cgatatggtg cgtcgcgcgc ggctccatat ccgggcgcag
     4861 aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca ccagcgccac
     4921 aaagctttcg ccattttcat tggcgcgttt gatgattttg tcgtcgatat cagtaatgtt
     4981 gcgcacatac ttcagcttat agccgaggaa acgcagatag cgcgcaacca cgtcaaaggc
     5041 aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa acggtgattc cacacacgta
     5101 catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg tcagagtatt
     5161 gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag ttgcgtataa
     5221 tacccatatt tcccgcgcga atcagcatac attgcgagat gatccgatcg tgcggttatg
     5281 ctataacacc accctatata tgacccgaac cgggttgaag caccaatcaa acggaacagg
     5341 atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa acttttgacg
     5401 ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt ttttacaaca
     5461 acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt tttgaaccgg
     5521 gcatgaaaca aaaagccacc aaagacccga tcaaaaacga agcgaacaac ggtctgaaaa
     5581 atacccgtgg tacgctggca atggcccgta ctcaggcacc gcactctgca actgcccagt
     5641 tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc ctgcaaggtt
     5701 ggggctactg cgtatttgct gaagtggttg aaggcatgga cgtggtagac aaaatcaaag
     5761 gtgtggcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac gttatcattg
     5821 aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc ttcatctctg
     5881 cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcagggg aggcccgcaa
     5941 ggccgatgcg ctgtatattc ttggcgatct gtttgaagcc tggatcggcg acgacgatcc
     6001 caacccactt catcaccaga tggccgcagc gatcaaagcg gtatccgatt ccggcgttcc
     6061 ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg cccgtgaaag
     6121 cggcatgacg ttattgccgg aagaaaaggt gctcgaactt tatggtcgtc gggtgttgat
     6181 tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc gcgccaaagt
     6241 ccacaaaccc tggctgcaaa cgctattcct cgccctgccg ttgtttgtgc gcaaacgcat
     6301 tgccgcgcga atgcgcgcga acagtaaaga agccaacagc agtaaatcgc tggcgatcat
     6361 ggacgttaac caaaacgcgg tggtcagtgc gatggaaaaa catcaagtgc aatggctgat
     6421 ccacgggcat acccatcgcc cggcggtgca tgaacttatc gccaatcagc aacctgcttt
     6481 tcgcgtggta ctgggtgcct ggcatacaga aggttcgatg gtgaaagtca cggcggatga
     6541 cgttgagcta atccattttc ctttctgacc acgaatggac agattctgaa aaagcaccga
     6601 actaacgcaa cctgtatatc ttttttaaaa tcagcaaggt aataatctct gagtgaaata
     6661 acaacaggga atattattat gcaacgctgc aacggatttt tgatttctgg catcgccctt
     6721 acggcaggag cgccactgtg tattagctgg ttcggtcttg tcggcttcta ttttggcgca
     6781 ttgtcactgg tagctgcggc ggtcatttcc tggatagtct atttctggct catttattta
     6841 gtactcaccc gcccccataa ccaacgcttt gtagaaagat atatattgat tgtcggtata
     6901 tgctttttac cgtggagcat tgtgctgata tgggcattct ttgccagttc agccagagcg
     6961 cataatcaac acgcagaaca aattaaaaac tcaacaactg acggttaatt ccgcctacgc
     7021 aaccgttttc ctagcactct ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt
     7081 gcaccacagg agttttaaga cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg
     7141 tgatggggtc caaaagcgac tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc
     7201 tgaatgtccc gcaccacgtt gaagtggttt ctgctcaccg cacccccgat aaactgttca
     7261 gcttcgccga aagcgccgaa gagaacggtt atcaggtgat tattgcgggc gcaggcggcg
     7321 cagcgcacct gcctggcatg attgccgcca aaacgctggt gccggtgctg ggcgtgccag
     7381 tacagagcgc cgcactgagc ggtgtcgata gcctctactc catcgtacaa atgccgcgcg
     7441 gcattccggt gggtacgctg gcgattggta aagctggcgc ggcaaacgcg gcgttactgg
     7501 cagcacaaat tcttgcgact catgataaag aactgcacca gcgtctgaat gactggcgca
     7561 aagcacaaac tgatgaagtg ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt
     7621 ttgcgtcctc ggtaacgggc agttaggccg tatgctgcgt caggcaggcg aaccgttagg
     7681 cattgctgtc tggccggtcg ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag
     7741 cgtgattacc gctgagatcg aacgctggcc ggaaactgca ttaacccgcg agctggcgcg
     7801 ccagccggcc tttgtgaacc gcgatgtgtt cccgattatt gccgaccgtc tgactcagaa
     7861 gcagcttttc gataagctcc acctgccgac cgcaccgtgg cagttacttg ccgaacgcag
     7921 cgagtggcct gcggtgtttg atcgtttagg tgagctggcg attgttaagc gccgcactgg
     7981 tggctatgac ggacgcggtc aatggcgttt acgcgccgct gaaaccgaac agttaccggc
     8041 tgaatgctac ggcgaatgta ttgtcgagca gggcataaac ttctcaggtg aagtgtcgct
     8101 ggttggcgcg cgcggttttg atggcagcac cgtgttttat ccgctgacgc ataacctgca
     8161 tcaggacggc attttgcgca ccagcgtcgc tttcccgcag gccaacgcac agctgcaggc
     8221 gcaagctgaa gagatgttgt cggcgattat gcaggagctg ggctatgtgg gcgtgatggc
     8281 gatggagtgt tttgtcacgc cgcaaggtct gctgatcaac gaactggctc cgcgtgtgca
     8341 taacagtggg cactggacgc aaaacggtgc cagcatcagc cagtttgagc tacatctgcg
     8401 ggcaattacc gatctgccgt taccgcaacc agtagtgaat aatccgtcgg tgatgatcaa
     8461 tctgattggt agcgatctga attatgactg gctgaaactg ccgcttgtgc atctgcactg
     8521 gtacgacaaa gaagtccgtc cgggccgtaa agtggggcat ttaaatttga ccgacagcga
     8581 cacatcgcat ctgaccgcga cgctggaagc cttaatcccg ctgctgccac cggagtatgc
     8641 cagcggcgtg atgtgggcgc agagtaagtt cagttaacct ggtgctcatt tgattgccgg
     8701 atgcggcgtg aacgccttat ccggcctacc gttccaacac cgcctgtaga cctgataaga
     8761 cgcaccagca tcgcatcagg catggtgtgc cgattgccgg atgcggcgtg aacgccttat
     8821 ccggcctacc gttccgacac cgcctgtaga cctgataaga cgcgccagca tcgcatcagg
     8881 catggtgtgc cgattgccgg atgcggcgtg aacgccttat ccggcctacc gttccaacac
     8941 cgcctgtaga cctgataaga tgcgccagca tcgcatcagg catggtgtgc cgattgccgg
     9001 atgcggcgtg aacgccttat ccggcctacc gttccgacac cgactgtagg cctgataaga
     9061 cgcgccagca tcgcatcagg catggtgtgc cgattgccgg atgcggcgtg aacgccttat
     9121 ccggcctacc gttccgacac cgcctgtaga cctgataaga cgcgccagca tcgcatcagg
     9181 ctagatgtgc caattgcctg gtgcccatca ctacagcgaa atacaagtcc ccgcttcgcc
     9241 cgccagcgtc tcttcaatcc gcgataacgc cccaatccac gcgggtttac cacggcttct
     9301 gacatagcca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     9361 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9421 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9481 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccgt cttccgtcac
     9541 aggcacacca ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9601 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccgac gtaaatattt
     9661 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9721 ataaaccgga ccgataaact tctctggctg taaaaacgcc ggatcatcag gcgaaacctc
     9781 aatgcgcgtc agcaccgtcg tgacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9841 cagcatatag ccaatcatcc cctggctttc cgccaccagt acatccagcg gatacggttc
     9901 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggtc cgttgccgtg
     9961 aacaattgcc agccgataag aacgggccag gcgcgccagc gcgggtacag cactggcaat
    10021 attgcgatat tgattttttg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
    10081 aagagccaca accagtgttt tcatggtttc ccttgtaata attgttggcc aagccagaac
    10141 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc gatcgccgct
    10201 gcggtacgtt tcgggcaact cagcgcatga acaaagtgca ggagtggcga ggcgaaatat
    10261 ccttgcgcgg cataacgtaa ataactgacg ctcaccgcag tggtaacgag ttgaagattg
    10321 tcggaacagg caaaaaacgg gcgaccggag cgcgcatcta aagcgccata ataccaggcc
    10381 gccagcagca ttccgctcag cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10441 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10501 tggcgtaatt cagcaggcag cggatcgctt gccgccaatg ccagtggccc gaaaagcccg
    10561 gtttcctccg cgcgttgcat ccatgcaacg gcaagcggtt gaggatgcgc aggcggcgta
    10621 atacgtagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag acgaatacct
    10681 tgtgccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10741 gcgcggcgaa gcacccatcc gccggggccg aaaccactcc cctgacgatg cagcgttaac
    10801 agttcgccgc tttccgtcat caggttaatc gcccgccgcc acactcccgc taaccgccag
    10861 gactggcgat aattcggtgc gctacggctg gcaagcagag gatggatgat cgtcattgtg
    10921 cgttcctcaa ccaatcccca tgctttcagc cagtgcttcc agcgcctgtt caaagcacgc
    10981 cagcggtgcc cgcacggtgc ctgcgccaat ctgcccgatc cccgcctctt tatgggcgat
    11041 accggtattg atgagtggcg taatgccggt ttctaccacg cgacgaatgt ccagccccag
    11101 gcacgccccc tggaaatccc agccggggat ctgcaactgc atattgcgtt caaggtaaat
    11161 ttccgccatc tcttcagata ccgctctggc cgcttccatg ccacccgcac cgacaaagcg
    11221 cgttacgcca ggcgctgcga tcattgccgc acctccgata ccaaaggttt cggtaatcgc
    11281 gctatcgccc atatccgggt tcgcctgctc ctgcgagaag ccggtgaaaa acagaccttg
    11341 cggagtgttt acaggcgcag taaaccagcg ttcacccagc ccgcttaccc gaatcccgaa
    11401 catattgccg ttgcgggtca tagccgtaac gatgctgcct gcgcggatca tcgcgcctgc
    11461 atccatcgcc gccttgcagt aggccatcgc gaggttgagg aagaactgat cggtcacgct
    11521 gagaaaatcc atcacttccg caatgtgctg tttatcatga tcgaggcgag caatttgtgg
    11581 ggccagcgta cgcattaaca gtgctgagga agcaatattg cgttgatgga actcatcgcc
    11641 catggtaatg ccctgcgcca tcattgccgt gagatcgata ccgcgctcca tgcgccccag
    11701 cgccgcgctt aatactggca ttaacacatc gcgcatccag cgatggcgag tcaggacatc
    11761 ttcgccgtaa gcgccaaaac gcatcacttt gccgatacct tcgttgaggt tgcagtacgc
    11821 ccggttgccg tcggtcacgt tctcgaccac cagcatcggc atactggcag aagtaatgcc
    11881 gcccattggc ccgacggcat tcacatggtg acaaggaatg aagttcactt ccccctgctc
    11941 cagtattgcc agcgcctgcg cttcatcttt cgcccagcct tcgaacagac atgcgcccac
    12001 gcacgcccct ttcatgggtc cggtcatctc ctgccagcgc attggcggcc cggcgtgaag
    12061 cagtgttttg cccttgttta gttcgctgat aagtgaagaa gccggttgca catccagcca
    12121 gtgtggacga gcacgacgaa tttgttcgat aaccgcagca ttggcttgcg ccactgatgt
    12181 aaacatggga accccttatt gcaaacgttc taataaacga gccagttttt tattgccgcc
    12241 ggcgactggc gaccattggt aatgcacaac cggtttgctg gcgctttgta gctccagcgc
    12301 aaagctgcgt aatccgatgt taatcacggc gacgttttcc agtaatgacg gtgtgtgttg
    12361 ctgtgtggca ggcgagagcg gatgaattaa cgccgcagcc agcaaggtgg cttccggtag
    12421 cgaactcact accgcgatcc ccgcatcttc cagcgtggcg atttgctgcg agcggcattg
    12481 cgggtcacgt tcagtgcctg tcaccgtggc aatggcatac agtggttgat tgtctgaacg
    12541 cgcggcacag gctttttgcc aggcgctcac cagcgaggcg gcaggatcgg cggtcgcgcc
    12601 gaagccaatc acgacatcaa gcagcaacac tcgcacttgc ggtttagcgc cgagatcggc
    12661 aattaacagg ttgcgtaagg tcgggtcgat catcggatgg ggacgcccga cggtgtaaaa
    12721 atcatcgccc aggtcgagga tctgatgtcc gtcggcgtcc agcatcatgc catgttgatg
    12781 ggtatcgtcg gcttccacgc caaggtgtcc ggcaagtaat cccgccgctt cagcagccag
    12841 cgtaccgccg gtatacaaac cgcaaataaa tccgctgctg acaggcgcta tcgcgttacg
    12901 tcgcgcagtg acgcgtgaaa gcagacaagc cagacgagcg gcctcatcca gcgaggaggc
    12961 aaaccagaca ttctcgtcgc gggccaccgc cggggtataa cctaaaaaca gcgccaccgt
    13021 cggtttgcca gttgctttca tggcattaac aattttcaga cgcacagttt cggcaggtgg
    13081 ttttgaaaca aatgccagca cttcgctttt ctcgtctgca ctgagcattt ccagcgccgt
    13141 tagcgcactg atgccgccca cttcacggct gagatcgcgc ccgccaagac caatcgcgtg
    13201 agtaattccc tcccctgcca gcgcaatctg tgagcacagc tcctgaatcc cggtaccgga
    13261 agcgcctatg acgccaatgt tgccttccgg catcacatta gcaaaagcca gcggcgtggc
    13321 agcaatcatc gacgtaccgc agtctggccc catcaccagc aagccttttt cccgcgcgcg
    13381 ggttttaagt tggatttcat cttccagcgt gacgttatcg gagaacatca tcacgttgag
    13441 gttgcgatcc agcgcctggt tcgccaactc cgccgcatac tcaccagcca ctgaaatcag
    13501 cgccagattg gcatcgggta atttctgact ggcactgtcc cagcgacgca cctgcgtcaa
    13561 cgcctggctg ctgcctgaac cctgcgccag ttgttttagc gccttttcaa gctgctgcat
    13621 aaccgcctgc gcgatccccg catccgccgc ttcgctacga attgccacgc aaatatcgtt
    13681 cggcgtggcg ttattaaaat cgtcatgcca gaaacctgta gtatctaata acgctttatt
    13741 ggcgggcgta cccatcatta cggaaacatc atcaacattt tctgattcgc tgagttttcg
    13801 tgaaataatc attaaactga ccgaatcctg aaaacaccct tttttaataa aggcgtggat
    13861 cataacaact ccttaaaaat tgcacccatg gcaattgcac gagcagtgtt aatgtggcga
    13921 ttacggtatc gatgacaacg agtaaataag gtgatgaata tcacacgggg attattaaca
    13981 aaagaattca aataaccgcc agactattac gcgttatttt ttaataagca tggttgtttt
    14041 aaaaaccagg ttaaaagcac gaagattatc aaataaattc taactatatt tttttgcctg
    14101 tctggatcac ataatccaga tattttccct gtatgttaat tgcagtcatg cttcacaccg
    14161 tcgttaaaaa ggaagacaga tgaaaatcag tcgggaaaca ctccaccagc taatcgagaa
    14221 taaactctgc caggctgggt taaaacgtga gcacgctgca accgtggctg aagtattggt
    14281 ttacgccgat gccagaggga tccactctca tggcgcggtg cgcgttgaat actacgccga
    14341 acgcatttca aaaggcggca ccaaccgcga accggagttt cgtcttgagg aaaccggacc
    14401 gtgctcggca attttacatg ccgacaatgc cgccggacag gtcgcggcga aaatgggtat
    14461 ggaacatgcc atcaaaaccg cccagcaaaa aggcgttgcg gtggtcggta tcagccggat
    14521 gggtcacagc ggcgcaatct cttattttgt gcagcaggct gcccgcgccg gattgattgg
    14581 catttcgctg tgccagtccg atccaatggt ggtgccgttt ggcggcgcgg aaatttacta
    14641 cggcactaac ccactggcct ttgccgcgcc gggagaaggc gacgagatcc ttacctttga
    14701 tatggcgact accgtacagg catggggaaa agtactcgac gcccgctcgc gcaatatgtc
    14761 tatcccggat acctgggcag tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt
    14821 acatgctctg ctccccgccg ctgggccaaa agggtatggc ctgatgatga tgattgacgt
    14881 cctctcaggc gtcttactcg gcttaccgtt cgggcgacag gttagttcga tgtatgacga
    14941 tttacacgcc gggcgtaatt tggggcaatt acatatagtt attaacccga actttttctc
    15001 ctccagcgaa ttattccgtc aacatcttag ccagaccatg cgcgaattaa ataccattac
    15061 ccccgcgccc ggttttaatc aggtttatta tcccggacag gatcaggata ttaaacaacg
    15121 caaagccgcc gtcgaaggca tcgaaattgt tgatgatatt taccagtatt taatttccga
    15181 cgcgctttat aacacgtcat acgaaacgaa aaatcccttt gcgcaataat tattgagaca
    15241 ggactttctt atgattacac atttccgtca agctatagaa gaaacgctgc cctggctttc
    15301 ctcttttggc gctgacccag cgggtgggat gacccgttta ctttattcgc cggaatggct
    15361 ggaaacccag cagcagttta aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga
    15421 tgaagtgggg aatttatacg gtcgcctgaa tggcaccgaa tatccacagg aagtggttct
    15481 gagcggttcg catatcgata ccgtggttaa cggcggtaac ctcgacgggc aattcggtgc
    15541 gctggcggcg tggctggcaa ttgactggct gaaaacgcaa tacggcgcgc cgttacgtac
    15601 ggtcgaagtg gtggcgatgg cagaagaaga aggcagccgc ttcccgtatg ttttctgggg
    15661 cagtaaaaat atttttgggc tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa
    15721 aggaaatagt tttgtcgatg cgatgaaggc ttgcggattt actctgccga acgccccact
    15781 aactccgcgt caggatatta aagcctttgt cgaactgcat atcgaacagg gctgtgtgct
    15841 ggaaagtaat gggcagtcaa ttggcgtggt gaatgcaatt gtcgggcaac gtcgctatac
    15901 ggtgacgctg aacggtgaat caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga
    15961 tacggtttac gctttcagtc gcatttgcca tcagtctgtc gaaaaagcga aaaagatggg
    16021 cgacccgctg gttctgactt ttggcaaagt agagccgcgc ccgaatacgg tgaatgtagt
    16081 gccgggtaaa accacgttca ccattgattg tcgtcatacc gatgctaccg tgctgcgcga
    16141 tttcacccaa cagttagaaa acgacatgcg ggcgatttgc gatgaaatgg atattggtat
    16201 tgatatcgat ttatggatgg acgaagaacc cgtgccgatg aataaggagc tggtcgccac
    16261 cctgacagaa ttgtgtgaaa gcgaaaaact gaattaccgg gtgatgcaca gtggtgccgg
    16321 gcacgacgcg caaattttcg cgcctcgcgt gccgacctgc atgattttca ttcccagcat
    16381 caacgggatc agccataacc cggcggaacg caccaatatt accgaccttg ccgaaggggt
    16441 caaaacgttg gcactcatgc tttatcaact tgcctggcag aaataaggag ttataaatgg
    16501 gatatttaaa taacgtcacc ggttaccgcg atgatttact ggctaaccgt gcgattgtta
    16561 aacacggtaa tttcgcactg ttaacaccag acggtctggt aaaaaatatt attccgggct
    16621 ttgtaaattg tgacgcgaca atcctctcca cgccaaagct gggtgcctct tttgttgatt
    16681 atctggtcac actgcatcaa aacggcggca accaacaggg cttcggtggc gaaggcattg
    16741 aaacgttcct gtatgtgatc tctggaaata tcactgccaa agccgaaggg aaaacatttg
    16801 ccttaagcga aggtggctat ctttattgcc cgccaggctc cttaatgacg tttgttaacg
    16861 cccaggccga agacagccaa atctttttat ataagcgtcg ctatattccg gtagaaggtc
    16921 atgcgccgtg gctggtttat ggcaatgcca gcgaactgga acgcattcat tatgaaggca
    16981 tggacgatgt tattctgctg gattttctgc ccaaagagtt aggttttgat atgaacatgc
    17041 atatcctctc ttttgcacca ggtgccagcc acggttatat cgaaacccac gttcaggaac
    17101 acggtgccta tattctttcc ggtcaggggg tttataacct cgacaataac tggatcccgg
    17161 tgaaaaaagg cgattacatc tttatgggcg cttattcttt acaggctggt tatggcgtag
    17221 ggcgtggtga agcgttcagc tatatttact cgaaagattg caaccgcgac gtagagattt
    17281 aagtcatacc actcgcctga ttatttttag ctatgttgga ttttgccacg gtttattccg
    17341 tggctttttt tgtagtcaat tcaggtaaaa aaagatgcga tataaatcgc atccgcttta
    17401 aaggtaatag tcagttttta attccctgac ctattttaat ggcgcaggca atatttcgcg
    17461 cactgttgaa gagattggtt tcaccgctgg cgagcacttc ggctaaagga gccagacgtg
    17521 gcaaaatgct gaataccgca tcaatgccgt actggtgcac cacttccacg ccatcaccca
    17581 atacgccagc aatcccaatc accgggacat taaactgctt cgccaccgat gccacaccca
    17641 gcggcgcttt accgcctgcc gtttgcgagt cgatgcgccc ttccccggtg atcaccagtg
    17701 ctgcaccctg cactgcctgc gcaagattga ccgcattcaa cacaatctca atacccggtt
    17761 taatatccgc attgaggaat accgccgccg caatgcccat accgcccgcc gcgccaccac
    17821 ccgccatctg gcagacgttg atttcagtta gctgttgtaa aacacgggcg taattttgca
    17881 gcccctgttc cagctcttcg accatctccg gcgttgcccc tttttgcggg ccaaacaccg
    17941 ccgccgcgcc gcgtgcccct accagcgggt tatcaacgtc acatgctact tcaatatgac
    18001 agttcgccag acgcggatcg cattcattca tctcaatact tgccacgcgc gctaaattgc
    18061 cgccgttagc cgccagcggc tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg
    18121 ccatgcccat accgccgtcg accgtcgcac tgccgccaat gccgagaata atatggcgaa
    18181 tgccattatc cagcgcatgg agaattaact cccccgtacc aaaactggag gccagcaacg
    18241 gattacgctt ttcaggcacg accagcatca ggccacttgc tgctgccatc tcaatcaccg
    18301 ccgttttccc gtcgccggta atgccataaa aagcattcac tttttcgccc atcggcccgc
    18361 agacttcaag cttcacgatg ttaccgtccg tcgcggcgac catcgcctcc accgtccctt
    18421 cgccgccatc cgctatcggc aaacagatat agtgcgcatc gggaaatatg gtcgaaaacc
    18481 cggctttaat tgcctgacaa catttttctg cacttaagct ctctttaaaa gagtctggcg
    18541 caatgacaat cttcattatc aatccttttt caacaaatta ttcgatgcgt tcccacggaa
    18601 taagattatc cataagaaca gccagtaaaa ttccgaccag taagccgtta ctgagcagag
    18661 gacgaagcgt taatggaagg tcttgcagat acacaggcgg taatgccatt aaaaatatgc
    18721 cgacaaataa cggcaatgcg agtcgataaa tattgcgagc ggtaaacgtt atttgctggc
    18781 taaacaccag cgcggaaaag agtaaaggca gataagaaac cagcatgacc gcactactca
    18841 ctggtaaagg gatactgcaa aacagtcgcg tgagtgccgg aaccagtgcc accagcaggc
    18901 aaataacgct gccataaata aacgaacgcc gcgagtaatc gcctgtctgg gttaataaac
    18961 caatggatga aacaaacggt gaaaatggaa ttactgccag cggcacggtt atcagtgtca
    19021 taaatccggt cgccacaaag ctgcgacgat aacgcgtatt ccccgcgccc tgctgcggat
    19081 aaaaaacatc cgtgccccga atcgcaccgt aggtattgct gatatttacc agacctgtaa
    19141 tcaccgccgt cagaataatc cccggcgaca aagcaccgcc actgccgagc gggaaccact
    19201 gccagtgcaa ctcaccggag agcgaatgcg aagaaggaaa gcaaaagtac cacaacaacc
    19261 agccagttat cgtgccgacc agcaggccgt aacgggcaaa acgttgtggc aggaaaataa
    19321 tcatcgccag taccaggcac atcaccgcca ccgagagcgc gaacggcggt aattgaattt
    19381 taaaattcgg ggcggctatg ccaaacggca gcccgagcat acctttgaaa aagatagtgg
    19441 tcagttgcgc acccagcatc aacataaaca agaccatcac cgacggcgta aataaccgtg
    19501 ccaggcgatg gcctaatccg ctaaaaccaa tcaacatcgt cagcacgccg gagagcgcaa
    19561 tccccaccgc cagactggta gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa
    19621 gggtgatagt caggatggtt ccccaccaca ggccgcccgg cccttccata atagcgcgac
    19681 gatgaccgca gaacgcctga gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg
    19741 tgagcgtaag caggctactt tgcggcaact gaaaagcaga aagtagcgta ggaggaacca
    19801 caaccgtgtt gcaaaaaata aagaaaaacc actggaatcc tgataacagg ctttcgcggc
    19861 ccactgcaaa attgaacata aacacacctt aaccggagaa tgcccgccac aaggacgggc
    19921 attgcagggc cagattactg ctgatgttta aggataaatt gacctttcgg cgcaacaggg
    19981 aagccctgtt cgatgtcgta aatcacatca ccacgtaaga tggttttcgt gatacgcgcg
    20041 ccaatagtac ggccaacata cgggctgact ttgtggcgat attccagatc gtcattggta
    20101 agaacatagc tgctattcgg ctgaatgaag acgaagtcgg catcttttcc tggggcgata
    20161 cggccttttt gctgcagacc gaaaatatct gctgcgttag tcgccattaa tttgccgaac
    20221 attggcagag acattccgcg tttctgtacc gcttcatcga acatcacgtc catgcagctt
    20281 tgcagaccag cgataccgcc ccacgctttc atgatgttac cggctttcat ttccggcggg
    20341 catggagagt ggtcggaaac caggcagtct atttcaccgt taaacagttt ttcccacatg
    20401 cctttctggt tttccagatc gcggatcggc ggtgaacact tcgccagagt accgatttct
    20461 tcgaactgat cggtatccag cacaaagtaa tgcgggcagg attcacaagt aacatcctga
    20521 ccttcctgac gtgcacgagt cacttcctca acaccttccg ggctgctgac gtggcaaacg
    20581 tgcagacggc aaccagcgac tttcgccagg tacagtacgc ggcgaatcgc ttccacttcg
    20641 gtaaataccg gacgcgaagc cacatagtca tgggcagtta cgcgaccttc acgcttcgct
    20701 tcttcgccca gtgcgtcaca gatcagcgcg ttttcgcagt gcaccagcac tggctggccc
    20761 agttcgccca gcttctgcgc gcctttgaag aattgccagt cattgacgtc acggaagtcg
    20821 ttgtcgatac cgcgatcgcc acaggtcgca acgaagcatt tgaaaccgac aacacccact
    20881 tcatccaact catgcagacg gtcaatgttg taagacacca ggccaccgag ttgtgccgca
    20941 tcgatagtca gctttccttt agcggcatcg aacttcagtt caattgaggc gcggtcaacc
    21001 gttgcaggca gctggttgag cggcatttcg atcatggtgg tgataccacc ttttgctgct
    21061 gcgcgagtac cggtttcata accttcccag tggctgcgac ccggttcaga aatatgggtg
    21121 tgcgcatcaa ccatgcccgg cgaaaccacc agaccagacg catccataac gtcttttgca
    21181 tcgcccagat cctggccgat agcagcaatt tttccgtctt taacggcgat atctacaacg
    21241 cgagcttcgt tttctaaaat aacggtgccg tttttaatga ttaaatcaaa agacataaca
    21301 aactccttgt aaaatacaga ttggttttta actcaatgtt caatatcgtg attaattaac
    21361 ctatggtttt ttgttctcct gttttttctg ctgttgtacg tttctttaat aaggcgtagg
    21421 ccgcaaaggc gacgatgacg ccgacaaacc atgaaacacg tgataacggt tccataaagg
    21481 gaataaactt accgccaaga gataaaataa cggccaccag agttactgaa aacgcggtga
    21541 ggttaaaacc gttatcgtaa tatttataat cgccaggtgc ggtgtacagt tcatcaagat
    21601 taatttgccc acgcatcacc acaaaataat gcgccatcat cacaccaatt accggaccaa
    21661 gcattccgcc gataatatcg aggaacagat aaatactgtc ctgattttcc attaatttcc
    21721 acggacagat cagcaggctg ataatactgg caatcagtac gccgttttta taggtcagtt
    21781 tcgtcggtgc aatggcggca atctgataac cagccggaat aatattaccg gtggcgttag
    21841 tagagatagt cgtcatcaga ataaccagta ccgcaaagaa cgaggcgaac aggctgtccc
    21901 aacgctgaac aatatccagc acgttccagg tgtcagcgcc gtaatgaata ctggctccgg
    21961 caataataca taccccggcg accgcaaaca gaatataggc cacaactaaa cccagcgttt
    22021 gccccagcgc ctgctcacga aacgagtggg cgttttgcgt aaagtcggat gcgctcaccg
    22081 ccggtgccgc ccagaccgca accaccgcgt ttatcaccac cagaaacagg aagccactgt
    22141 tttctgcttt ctgaataccg cccggaatgt agtcaaagat tggaccgatc ccgaccagcg
    22201 aaatcgccca aatcgccata ccgccgaaaa cgatatagat gcacgggtta agaatggcag
    22261 tgaatttatt taaaactttt ccgccgccaa aaccaatacc aacgttaacc agccagaaga
    22321 gtaagaaagt aattaatccc ggtagagaaa ggcctaacag cgtgaaatcg ccaccgagag
    22381 tcaaaaatcc cggccagatt ttgccaatca gaatcaagca ggccagtgac cccgcgtaac
    22441 attgcaggcc aaaccacata atggcagcaa taccgcctct taataatccg ggaaacagtg
    22501 caccacgtac gccgtaagaa gcacgcagga tcatggcaaa cggtacgccg tatttactgc
    22561 ccgcagcacc gtttaatacc attaccgcgg caatgaaaaa ggcactgagg ataattgcca
    22621 gcataatact aaaggtagac aagccgagaa taaaaaagcc gccaaccatc acataattgg
    22681 gaacgttatg aaccgaaccc atccataagg taaaatagtt aaatgttttc caggtccgct
    22741 gactttgcgt tttcggcagt agatcttcgc tatagccgcg ttgctggaat agttttctct
    22801 gatgttccat aaatccctct taatttcacg tatcgaataa cgttgtttta atctagctat
    22861 gaggaacaaa taaaataaaa atattatttt taatactgaa gcaacatctt tggctgcttt
    22921 ctttttttaa tattgctggc cgattcattt tctgatatcg gccattttta ttgcgggtat
    22981 caggccagtt tatggttagc cattaattcc agcgcctgca ctaatgcaga gtgatccaac
    23041 tggctgccac cgtttgccgc acaggtatta aacaattcct ggcaggtcgc ggtgtttggc
    23101 aggttcagcg ccagtgcttt cgcactttgc agtgccaggt tgagatcttt ctggtgcagc
    23161 gcaattttga aacctggatt aaaggtgcgt ttaatcatgc gctcaccgtg aacttccaga
    23221 atacgtgagg aagcaaagcc gcccatcagc gcctggcgca cacgtaccgg gtccgcaccg
    23281 gcttttgaag caaacaacaa ggcttcagaa accgcttcaa tattgagcgc cacgataatc
    23341 tgatttgcca ctttgcaggt ttgaccgtcg ccgttaccgc ccacgagagt gatattttta
    23401 ccgagcagtt caaacagcgg ttttacacgt tcaaatactg cttcatcacc gccaaccata
    23461 atcgataacg tcccttcacg cgcgccgatt tcaccgccgg agactggcgc atcgagataa
    23521 tcgccgccca gttcattcac ctgacgagcg aaacgtttag tttcaatcgg ggaaatggag
    23581 ctcatatcaa caatggtttt gcccttcagc gaggctttgg tacaaccatt ttcaccaaac
    23641 agaacgtctt caacctgtgg cgtatccggc accataataa aaatgatgtc cgatgcatct
    23701 gttacctggc gcgcagtttc aacactaacg gcgcccagtg acagtaattc atcagcaacc
    23761 ggaccaatgg ttgtgacatg cagttgatga ccggcacgcg ccagattaat ggccatcggt
    23821 gtacccataa tgcctaagcc aataaatccc agtttcataa ttaacctctt ttaaatttcg
    23881 cttttcctga attcagacaa cacgatcccc tgccaactta gcggcctaaa aaagcattgt
    23941 ctgaatagcg ttacgtttta acggtacggg tccatccagc gtaaaccggc ttccgtggtg
    24001 gtttggggtt tatattcaca gccaacccaa ccgttgtagt cagaattttc aattaccgta
    24061 aagagatatt cgtaattaat ttctccggtt cccggttcgc cgcgatgcgg attatcagca
    24121 atttgcaggt gaccaatttt atcggcccac tgagtcatgg tattggttaa ttcaccttcc
    24181 atccgctgca tgtgataaat gtcatactga attttcaaat tgcagcaacc aacatcgtca
    24241 atcagtttca gtgcctgccg ggttccggtg agatgaaaac caggaatatc aaaatggtta
    24301 ataggttcaa tcagtaataa aatatcttct ttcatcagca tattcgcagc ataacgcagg
    24361 ttttctacaa gcgttgtgtg aatctgttca ctgctgaaac cagtcggtgt tttaccgacc
    24421 agacagttaa tttttttatt acccagcgca cgagcataac gaatcgctgc tgctacgcca
    24481 tcccgaaact cttcttcacg gccaggaata caggcaatac cccgctcgcc cgccgcccag
    24541 tcacctgccg gtaaattgtg cagcgtatgt tcaagtttat tactcgccag cacctgtttt
    24601 aattcttcaa tgtcgtagtc ataagggaac ataaattcaa cgccgcgaaa accacactgc
    24661 gcagctttct caaaacgggc gagaaaatca tattcaccaa ataacatcga taaattagca
    24721 gagaaacgta acattattta tctcccttat tcatagtgca tgaaacaggt ttcagtcggt
    24781 gcatccgctg cgttatcggc gatatcttca aattccatga cgttatccag ttcactgccc
    24841 atcgaaatat tagtcacacg ctcgagaata acttccacga ctaccggtac ccgatattgc
    24901 gccattaaga ttttcgcctg ttcaaaggct ggtgcaatat cttccggttt gaaaacgcga
    24961 attgctttac aacctaaacc ttccgctact tttacgtggt cgacgccata accgttcact
    25021 tcgctggagt taatattttc gaacgcgagc tgtacgcagt aatccatatc aaaggcacgt
    25081 tgcgactggc gaatcagacc cagataggcg ttattgacca gtacatggat gtacggaatg
    25141 ttgaactgtg cgccaacggc taattcttca atcaggaact ggaagtcgaa gtcaccggaa
    25201 atcgccacca cattgcgctc tggatcagcg gcacaaaccc ccagcgccgc cggaatcgtc
    25261 cagcccagcg ggcctgcctg cccacagttg atccagtggc ggtctttaaa gacatgcagc
    25321 atttgtgccg cagcgatttg tgacaggcca atggtggtga cataacaaac atcgcggcca
    25381 aaggctttgt tcatctcttc atacacgcgc tgcggtttca ccggcacgtt gtcgaagtgg
    25441 gttttgcgca gcaaagtgcg tttgcgctgc tggcagtcgg cgacccactc tttacgacac
    25501 ggcagacgac cggctttttg catctcctgc gccacttcaa ccagcagcgt cagcgccgct
    25561 ttagcatcag agacaatgcc aagatccgga cacagcacgc ggccaatttg cgtcggctca
    25621 atatcgatat gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc ggtatgacgg
    25681 ttagcaaaac ggttaccgat accaaacacc atgtcggacg ccagcaacgt tgcattaccg
    25741 taacgatgcg cggtttgcag gcccaccatc ccggccatca gttcatgatc gtccgggata
    25801 cagccccagc ccatcagcgt tgggatcacc ggaatgctgg tcagttcagc aaactgttgt
    25861 aacagtacag ctgcgtcagc attaattacc ccgcctccgg caacaatcac cggacgttcg
    25921 gcctggatta acatttctac agctttttcg atctgcatac ggctggcagc aggtttgtag
    25981 accggcagcg gttcgtacat atcaggatca aactcgattt ccgcaacctg tacgtcgaac
    26041 ggtaaatcca ccagtaccgg acccggtcga ccagaacgca tcaggtgaaa cgcctgttgc
    26101 agcacacgag gcaccagcgc cgcttcacga actgtaaccg ccattttgct gaccggttta
    26161 gcaattgctt caatatcaac ggcctggaaa tcttctttat gcagacgggc gcgcggagcc
    26221 tggccggtaa tacacagaat aggaatggaa tcagcagatg cggaatagag cgcggtgatc
    26281 atgtccgtgc ccgcaggacc ggaagtcccc agacatacgc cgatattccc tgccgttgcg
    26341 cgggtataac cttccgccat gtgcgaagca ccttccacat gacgcgccag aatgtgacga
    26401 ataccgccgt gcttacgcat cgctgagtag aacggattga ttgcagctcc cggaacgccg
    26461 aaggcggtgg tgataccttc tttctccagc acatacattg ccgcgtcaac ggctctcatt
    26521 tttgccattt tattcctacc tctattaatt ggaaaaattt tccaactttc aaattttgtc
    26581 aaaaaaaccc gctccgcaga gcgggatacg acagacacta tagatgtgct ttcagtccca
    26641 acgccgtgct gatatcgcgg gcggtgtctc tgaccagctc accctgactg acaaaacgat
    26701 cttctgtcag tcttgatgaa ggcccggaga tggagatagc ggcaacaaca ctacctacat
    26761 catcgtaaat tgctgaagct atgcaattca ggccgacaac atgctcttct ttatctacgg
    26821 tatagcccag ttcacgcgct tgttccaggt ccttcagcaa ggtgggcata tccacaagtg
    26881 tcgttggcgt aaactgctgc aaaccggttt gcagaatgat gctcatcaac tcttcttccg
    26941 ccagcggata aagcagcgcc ttgcccgctc cggaagcatg cagcggcaga cgactgccca
    27001 gcggcgcaca catcctgacc atcgatttac actctaactg accaattaat accgcttcat
    27061 tgccgttacg gatcgcgaca ttgaccgttt cgccggaaag taacatcagg cggcgcataa
    27121 acggcccggc gacggagagg acatcgcggt tatggatgta cgctgcaccg acgttaaaga
    27181 cacctaatcc tatatgccac cagcctaact gattgtcctg atagacaaaa tccgctgcct
    27241 gtaaaacctt cagcaagcga aaggtcgtgg agagcggcaa atccagattg agagagatat
    27301 cgctaaccga cgaacttccc ccactttttt ccagatattg cagaatcgca attccccgct
    27361 ctaacgcctg tgcgcccttc tgtgctacag gctccgcctg tcctggcctg ccgcgccgtc
    27421 taacttccgt catacctacg ttaactccga cctacaaatg agcataaaca atgaacaatt
    27481 cgtttatctt atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct gttcaggaat
    27541 actttcaaca tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat cggtgacgcg
    27601 ctgccaggcg aaaagtgggt gatgccagac gttacgatgg taattcactc cctgttcgcc
    27661 attggtaata aacgcccgca gggttgacag gtctggtttg tcgtcaccta acgccaccac
    27721 caccacaaac acttcacctt tcatcgggat aaacgcctgt gtacccagcg gatgacgttc
    27781 gagttcgtga atggtcagcg gcagattcgc cggttgcgcg cggttaatgc tgataagcgt
    27841 acggtcttgc tcaagaatct caaccagcgc caaatcgtgg taacgctcca ccagtccatt
    27901 gttaatatgg aaaaaatccc gtttctgcgt ttcgattacg tcgccataag cactaaaggc
    27961 ttcctgactt aacggtaata cctgaagttt catctttttg tccttgtaac ataattccgt
    28021 tcaaccaccg ccgaccggtc agcttggttt caataacagg caatctaaaa ccttaagaga
    28081 acaacatcaa cacaaaagtt ggaatatttt tccaaaacta gatttagtcc acaaattgaa
    28141 cagggttatc atgtaaaaat actgataaat cagaggataa gaaaatcacg caacttgcgg
    28201 atttaaaagg ggatacctga agatgttcga tccagaaacc ttgcggactt tcattgcggt
    28261 tgctgaaaca ggtagttttt caaaagcggc agaacgatta tgtaaaacca cggcgacgat
    28321 cagttatcgc attaaacttc tggaagagaa taccggagtg gcgctgtttt tccgcacgac
    28381 tcgcagcgtg acgttgacgg cggctggcga gcatctactt tgtcaggcca gagactggct
    28441 gagctggctg gaaagtatgc ccagcgagtt acaacaggtg aatgatggcg tggaacgcca
    28501 ggtgaatatc gtcatcaaca acctgctcta taacccccag gccgttgcca ggctgctggc
    28561 gtggctaaat gaacgttacc cgtttaccca atttcacatc tcccgacaaa tctatatggg
    28621 cgtctgggac tcgctattgt acgaaggttt ttcactggct atcggcgtca cgggaaccga
    28681 ggcgctggca aataccttta gtcttgatcc cttaggatcg gtgcaatggc gctttgtcat
    28741 ggcggcggat catccgctgg cgaacgttga agagccgcta acagaagcac agttgcggcg
    28801 ctttccggcg gtcaatattg aagacagcgc ccgcacctta accaaacgcg tcgcctggcg
    28861 attgccgggg caaaaagaga ttattgttcc cgatatggaa acgaaaatcg ccgcccatct
    28921 ggcgggcgtt ggcattggtt ttttgccaaa atcactttgc cagtcaatgc tcgataatca
    28981 acaactggtc agccgggtaa tcccaacgat gcgccctcct tcgccattga gtctggcatg
    29041 gcgcaaattt ggcagcggca aagcggtaga agatattgtg accttgttta cccagcgcag
    29101 gccggaaatc agcggatttt tagaaatttt cggcaaccca cgcagttaaa aatcaagttt
    29161 ccttgcgcgt tggcgcgtaa aatagcgcgc aagctgattt gaggttcact ttttactatg
    29221 caagagagac acacggaaca ggactatcgt gccctgctga ttgctgatac gcccattatt
    29281 gatgttcgcg cccccatcga gtttgagcaa ggcgcaatgc ccgccgctat caacctgccg
    29341 ttaatgaata acgatgaacg cgccgccgtt ggcatctgct ataaacagca agggtcagac
    29401 gcggcgctgg cactggggca taaactggtg gcgggtgaaa ttcgtcagca gcgcatggac
    29461 gcctggcggg cagcgtgcct gcaaaatccg cacggtattc tctgctgcgc ccgtggcggt
    29521 cagcgctcac atattgtgca aagctggttg catgacgcgg ggattgatta tccgctggtg
    29581 gaaggcggtt ataaggcact gcgccagacc gcgattcagg cgactattga actggcacaa
    29641 aaaccgatag tgctgattgg cggttgtacc ggcagcggta aaacgctgtt agtgcagcaa
    29701 cagccgaacg gtgttgatct ggaagggttg gcgcgtcatc gcggttcggc gtttggtcgc
    29761 accttacaac cacaacttag ccaggcgagt tttgaaaaca tgctggctgc cgaaatgcta
    29821 aaaaccgacg cccatcagga tttgcgcctg tgggtgctgg aagatgaaag ccggatgatc
    29881 ggttcgaatc acctgccgga atgtctgcgc gaacgaatga ctcaggcgac gattgcggtg
    29941 gtagaagatc cgtttgagat ccgtcttgaa cgcctgaacg aagaatattt cttgcgtatg
    30001 catcatgatt ttacccacgc ttatggcgac gaacagggct ggcaggagta ttgcgaatac
    30061 ctgcatcacg ggctttcggc gattaaacgt cggctgggac tacaacgcta taacgaactg
    30121 gctgcaaggc tggatgcggc gctgacaacg caactcacca ccggcagcac cgacggtcat
    30181 ctggcctggc tggtgccgtt acttgaagaa tattacgacc cgatgtatcg ctatcagctt
    30241 gagaaaaaag cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc ggaatgggtt
    30301 aagacttatg cctgtggtta ttaacccaca aaaatgcttt tccgttcctc tttaataaat
    30361 tgtgcaactg gttaactacc agttgccata gggatatttt ttatctttga taaagttttt
    30421 tgtactttcc ggataagaat acccatgttt ataggcactt cggccgttac acatttccat
    30481 tttttcacct gaaaccgttt ctatcagaat ctgcttatcc gtcaggcgaa tattgggctt
    30541 gtcaggtttc tccagccaca ctcctatttt cccttccgga gccagcccga ttaacagata
    30601 ccgcagataa tacttttcac tgccaccgcc aggtggtgaa taaggtgtga gcatcaattt
    30661 ccagacgtct cccgccagcg ttatccaggt ttcatactct tttttatcaa tcactgaatc
    30721 ccagcagaaa tgg
//
LOCUS       DAJOTB010000045        30238 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJOTB010000045 DAJOTB010000000
VERSION     DAJOTB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.045"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(105..383)
                     /locus_tag="OHN28_001997"
     CDS             complement(105..383)
                     /locus_tag="OHN28_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297396.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(742..1032)
                     /locus_tag="OHN28_001998"
     CDS             complement(742..1032)
                     /locus_tag="OHN28_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2297397.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1033..1344)
                     /locus_tag="OHN28_001999"
     CDS             complement(1033..1344)
                     /locus_tag="OHN28_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2297398.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1573..2481
                     /locus_tag="OHN28_002000"
     CDS             1573..2481
                     /locus_tag="OHN28_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2297399.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2545..3498)
                     /gene="fabY"
                     /locus_tag="OHN28_002001"
                     /gene_synonym="yiiD"
     CDS             complement(2545..3498)
                     /gene="fabY"
                     /locus_tag="OHN28_002001"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCQ2297400.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPTTTPIRHFLMIKPVATLDDILHRGD
                     WCAQLQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATL
                     TGWGLIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKA
                     RVQMQVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3543..3980)
                     /gene="dtd"
                     /locus_tag="OHN28_002002"
     CDS             complement(3543..3980)
                     /gene="dtd"
                     /locus_tag="OHN28_002002"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCQ2297401.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3977..4849)
                     /locus_tag="OHN28_002003"
     CDS             complement(3977..4849)
                     /locus_tag="OHN28_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCQ2297402.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4843..5442)
                     /gene="yihX"
                     /locus_tag="OHN28_002004"
     CDS             complement(4843..5442)
                     /gene="yihX"
                     /locus_tag="OHN28_002004"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCQ2297403.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLRAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5583..6470)
                     /locus_tag="OHN28_002005"
     CDS             complement(5583..6470)
                     /locus_tag="OHN28_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCQ2297404.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMLLTSPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6460..7809)
                     /locus_tag="OHN28_002006"
     CDS             complement(6460..7809)
                     /locus_tag="OHN28_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCQ2297405.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7908..9341)
                     /locus_tag="OHN28_002007"
     CDS             complement(7908..9341)
                     /locus_tag="OHN28_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2297406.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9411..10421)
                     /locus_tag="OHN28_002008"
     CDS             complement(9411..10421)
                     /locus_tag="OHN28_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCQ2297407.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10454..11341)
                     /locus_tag="OHN28_002009"
     CDS             complement(10454..11341)
                     /locus_tag="OHN28_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCQ2297408.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11366..12244)
                     /gene="yihU"
                     /locus_tag="OHN28_002010"
     CDS             complement(11366..12244)
                     /gene="yihU"
                     /locus_tag="OHN28_002010"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCQ2297409.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12417..13313
                     /locus_tag="OHN28_002011"
     CDS             12417..13313
                     /locus_tag="OHN28_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2297410.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13353..14141
                     /locus_tag="OHN28_002012"
     CDS             13353..14141
                     /locus_tag="OHN28_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2297411.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14249..16072)
                     /gene="typA"
                     /locus_tag="OHN28_002013"
     CDS             complement(14249..16072)
                     /gene="typA"
                     /locus_tag="OHN28_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCQ2297412.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            16445..17854
                     /gene="glnA"
                     /locus_tag="OHN28_002014"
     CDS             16445..17854
                     /gene="glnA"
                     /locus_tag="OHN28_002014"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCQ2297413.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18028..19077
                     /gene="glnL"
                     /locus_tag="OHN28_002015"
     CDS             18028..19077
                     /gene="glnL"
                     /locus_tag="OHN28_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCQ2297414.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19089..20498
                     /gene="glnG"
                     /locus_tag="OHN28_002016"
     CDS             19089..20498
                     /gene="glnG"
                     /locus_tag="OHN28_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCQ2297415.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            20610..20720
                     /locus_tag="OHN28_002017"
     CDS             20610..20720
                     /locus_tag="OHN28_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCQ2297416.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(20953..22326)
                     /gene="hemN"
                     /locus_tag="OHN28_002018"
     CDS             complement(20953..22326)
                     /gene="hemN"
                     /locus_tag="OHN28_002018"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCQ2297417.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(22515..23024)
                     /gene="yihI"
                     /locus_tag="OHN28_002019"
     CDS             complement(22515..23024)
                     /gene="yihI"
                     /locus_tag="OHN28_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCQ2297418.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            23606..24238
                     /locus_tag="OHN28_002020"
     CDS             23606..24238
                     /locus_tag="OHN28_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCQ2297419.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(24620..27406)
                     /gene="polA"
                     /locus_tag="OHN28_002021"
     CDS             complement(24620..27406)
                     /gene="polA"
                     /locus_tag="OHN28_002021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCQ2297420.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            27770..28702
                     /locus_tag="OHN28_002022"
     CDS             27770..28702
                     /locus_tag="OHN28_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCQ2297421.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVRGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(28743..30173)
                     /locus_tag="OHN28_002023"
     CDS             complement(28743..30173)
                     /locus_tag="OHN28_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2297422.1"
                     /translation="MIRKSATGAIVALSVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTKLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNTAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKQSDKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         7516 a         7798 c         7345 g         7579 t
ORIGIN      
        1 gatggtgtac ccgaaaaagt cagcagcttt accgcaccag gggttaaggt gaaatctacc
       61 attcataacg accggtcagt gccgcaccaa ccggtcttta attgttagta gtagtaaatt
      121 tgtggataac agtccgggaa ccagacgatt ccttccggat catccccctg cattacccga
      181 tgacattggc gtcgatcatc tgcgcgacgg cgaagcgtag tatagagccg cttccattca
      241 tgatcgcccg gtgtactgta gtttacccaa caatccattg aagggtgacg ccatttacct
      301 gtaagtgttg ggatccctga atgcttatgc ggcttcgctt taccattacg atccctatct
      361 ttacgcgtat taaacgtgga catttttagt ctcctgttaa gagttctaaa aatgtgcttc
      421 cttaaaatag ttcatcattt tcccttaatt cgcatagcgt gcttttgcac gaatttccga
      481 tacgcgggga ccattatgat agcgtcgatg cccacacatc caacaagaac aaatacaggg
      541 agtacgaaag caaatacctg atgctttttt gcttccatcg cctgcagtgc gataacgaca
      601 gcggattttt tttaatcgca ccatatgatg gcgacgttca gcacgattac gggtcatagc
      661 aaaactcctg aacaggcggc actatgccgc tttcatagga gttctgaatg ctggtgcttt
      721 ctgtatggca taaccatgcc tctaacggtt aagtgcttgc agcacatgtt gcggatcatt
      781 ggcaatcgct cgcagcaaag cctttgcagg cccggtcggt gaacgtcgcc cctgctccca
      841 gttacgcaac gtgtctacgc ttacggaaat aagatcggca aatttagatt gtgacaaccc
      901 tgaagcgcgg cgtatttctt tgattttcat agcatcaact tcaaaagtcc gtgaaggctg
      961 tcgcgttcct tcaataattt cattcatctg ggccatgctg tgagtcaacc gctcaaaaag
     1021 tgccttatcc atttaccacc tcgtatttaa caaccggagc attgcttttt cctggggaga
     1081 cagatcatct ttaataccct tacgataaat aagcagtaat ctgatctcat aatgtttaac
     1141 ctgatgaaag taaataaccc tgacaccagc acgtttcccc tttcctcctg agacccatcg
     1201 aacctttctt aacccaccag tttcaggtat tacctcacca tagtcaggat tcaaggccag
     1261 gaaaaactga aaacgactga attcatcgtc gttcagcagt ttttgaacat cttcagtaaa
     1321 aatttccgtt tcgatgaaga gcataattat acgccattgg cttagagcaa caatcctttt
     1381 tgtacgtcat tgacgtacca ttgaaaccac tagcttactg atagtgctcc agcaaaacca
     1441 cgtctctgcc gggtttgtct ttacatttgc gaggcgcttt ccagattttt cacgcccgtg
     1501 aatttccctg tatccatcca gcaaaaacgg gtgtataaaa ctgtacattc atacagcaaa
     1561 atgagttcta ctatggcact ggaaaaagga attgcagagt tagtcgaaga gtttatcgct
     1621 gcgggtcgcc cctcatcgcg ccaacaaagt attacgcagc gcagagaggg ctatatcgcc
     1681 agcgctgtcc ttgcgggtga gacagaaacg cgggtagata ttcagactat cgaacttgaa
     1741 gggatgacct tacgaatagt gtcaccgctg aatgccccga cattactccc gacgataatt
     1801 tattactacg gtggctgttt tgttagcggc gggttcgcaa ctcacgacaa ccagctgcgc
     1861 cagttagcct attacgggca atgtcgggtt attgccgtcc agtatcggct ggcaccggaa
     1921 catactttcc ccgccgccca tgatgatgca caacgaggtg cagaattagt ccggcaacac
     1981 gccgaacgac tcggcgttga taaacaacga ataactcttg ccggtgacag tgcgggaggg
     2041 catctggcgc tggtaactgc tttgcgattg aagcgtgctg gcgagtggca accagcacaa
     2101 ctcatcttaa tttatcctat gcttgatgcg actgcgcatt tcgaaagcta catacgcaat
     2161 ggtcacgatt acatcatcac acgcgacact ttactgagcg gctttgagat gtacctgcca
     2221 ggcattgaac gcaggcatcc tgaagccagc cctatttggc gcaatgattt caatggcttg
     2281 ccacctgtac atatcattac cgctgaatac gacccacttt gcgatgaagg tgaagcgttg
     2341 tatcaccgca tgacagggca aggagtgcaa tgcacctgcc aacgctacct tggcgttatc
     2401 cacggatttt ttcagttagg tggtatcagt gaggctgcgc gatcggcgtt acgggatgtt
     2461 gcctggagag cgggaaggta actccggacc agggcccaga aaaggttttc agattgaaaa
     2521 ccttttcagt caagtaaaac gcccctactc ttcttcgttc ccgccctctt catacgggcc
     2581 aaatggcttc gcgggcagaa cgatatacgt gccttcaaac actgcacccg gcgtctcgtc
     2641 gccaaagatt tctacttgca tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag
     2701 atcgccactt aaggcaccaa ggtcggctac cgcatggggt tttccgctaa tcggcttgct
     2761 gtagcggata tgcgcatccg ccagaataat cgttccgccg aggtggcgtt cgcgcagcat
     2821 cagccagata agcccccaac cggtgagcgt agccagcgag aataaactcc cggcaaacag
     2881 cgtgtgatgc ggattctgat tgccggtttc cggcatggtg gtgataaatt tttgcccggt
     2941 atattgctga atgcgcacac ccattttttc gcttaacggg atatgttcat accacgcttg
     3001 ctggagctgg gcgcaccagt cgccgcgatg aagaatatca tccagagtgg cgacgggttt
     3061 aatcatcaga aaatggcgaa tcggcgtggt ggttggcgtg gtggttggcg tggtgatttc
     3121 accctgatta acaaacccca gcttggcgaa aaactccacc gcgtcttcac gggcgctaca
     3181 ggtcacgcgc ttaacgcctt cctgacgtgc caccgactcc agggtcatcg ccatcagcgt
     3241 gcctaaccct ttgtcctgca cgtcgggatg aacggccata aagcgaatgg acgcttcatt
     3301 gtcggcatta atatacagtc ggcctaccgc taccagatta ccctgctcgt cgacgaccat
     3361 ctggtgatgc gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg
     3421 cttacgcaac atttcccagc gaaactgata gtaacgctct aattcttctt ctgtttgtgg
     3481 aacccgaagg tgatacatag cgatactctc tcttgtttcc cgtgacaacc ctggaagctg
     3541 gctcatacct gcaaccagaa tgtcacgggg ccatcattga ccagcgatac ctgcatatcc
     3601 gcagcgaagc gtcctgtttg cgtgttcata tcttgctgac ggcagcgttc gacgaaatag
     3661 tcatataacg cctctgcgcg atccggtgat gcgcctttgg agaaacttgg gcgcatcccc
     3721 cgttcggtat ctgcggcaag ggtaaactgg gaaaccacca gtacactgcc gcccgcctgt
     3781 tgcacgttga gattcatctt gccttcggca tcgctaaaga tgcggtagcc gagtacacgc
     3841 tcgcacagac ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa
     3901 agtcccgcgc caatttcacc cgtcacttct ccctccacgg tgacgctggc acgggttacg
     3961 cgttgaatta atgcaatcat ggttcgtcgt cttcttcttg ttcagcagct tgttttaatt
     4021 tgcggtattc cccgagagtg acagtaattt ccgcgccaag caagacgata caccatgtcc
     4081 agtagaccca aacaaagaga atggggatca ccgccagcac gccgtaaatg agctgatatg
     4141 acgggaacat ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac aggagcgcgg
     4201 cgacaaacgc gccgacaatc gcgtcgcggt taggtacgcg aatggtagga acgatgctgt
     4261 acagcaacca aaaggagatc cacgataaca gcagcggaaa aatacgcagc acgttgtcga
     4321 tgacagtatt gagatcgctc gcccagcgca gggaaagcag ataggagctg atcgccagac
     4381 tggcccctgc cagcagcggc cccagcgtta aaatcatcca gtacacggca aacgagtaaa
     4441 ttttgggtcg cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg atggagtaca
     4501 tcaacaataa cgccgtgacg atcagcccgc acgccccgac ggcggtcatt ttgttggaat
     4561 tggcgacaaa ctgttcgata tatcgctgga taacatcgcc agtagcaggc agaaagttgg
     4621 caaaaataaa gtgacgcaac tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa
     4681 gcgcaaaaac aacggcaacc agcggcacta atgagagcaa cgacacatag gcaaggttac
     4741 ctgccagggt tgtcatgttg tcctcatcaa tgcgttgcca gagtagtttt agccaggccc
     4801 atagtggacg ggtacgatgc ctggctttgt cctgaatggt ttttagcata acaccttcgc
     4861 gaaatagtcc gggatggtgg ttttatcttt caccagaata ctagtaatgc ctagccgatt
     4921 agctccttct atattatcgg cgttatcgtc gaaaaagacc gtatcgtctg gtgaaaaacc
     4981 ttctgcccgc aaaacatgct gataaattcg tgcttcaggt ttgcgcatac ccaagtcttg
     5041 cgacagatag atatggtcag cagcatcacg gatttccggg tattcctccg gccagaaggt
     5101 agtatgcagg cggttagtat tggaaagcac caccacgcga tgcccctgct cacgcagttt
     5161 atgcatgatg gcgatcactt ccggtcgtag cgcaacaaat accgcctgcc aaccgtggga
     5221 gaactgctca tagcttagcg gcagggccat ctcatgacac agtgcctctg cgaacgcttc
     5281 gtcgctaatt tccccacgct catgctgatg aaacgcctcg cccatgtgga aactcttctt
     5341 aagcgtagcc agtggaacac gcgttaaatc gctccaggcc cccagcacac ggttaaagtc
     5401 gatatcgaca atcacattac ctaaatcaaa gatatagagc atttttgcct cctttcgcgc
     5461 cacaagaaat taactgtagc gggaaaggat gggtttggct atgtggcaat aagggtaatc
     5521 ttgtgatgct gattgcgaaa aagcagagta atttcactgg ctttttcgca atagatgaat
     5581 ggttaagatt caggtgtaaa ttgccaacga gtttcgatga ccagcggctt atctggtgtc
     5641 acaaattccg gcgttagatt tattgcgtta ggtgcctgag tcagcgggtt aatacaggtg
     5701 gcatcgggct gtttatcaaa taccaccaga ctgtttgcag aagaggtcat ggtcatcgcg
     5761 agtttaccag gccacagtaa cttaaccttc accccgtcat aaaaaccaaa acagtcatcc
     5821 cagggaccaa cttgaggaga gatacgattt ccagttggca gttcatcact acccgcttct
     5881 tcttgccagt ccgcatcaaa aagcacctgc aacgattctg tattttgcgg agtcagcttt
     5941 ttcgcaaacc acggatgcca gcctgcggag gccgggaatg tatcggcata tgaatggact
     6001 tccagttgca aaaccagcgc atcgttctcc agcaaaaaag tctgaatcac ttccccttgc
     6061 cagggccacg gtgaagtcag gagcatacgt aaagtcaggg aatctgctgt tttgtcgatg
     6121 atttcccacg ttgagtaaca agccatacca tgcaacgcat gtggtggttt attggcgggt
     6181 aaagaataac attgcccacc agcattcagc gtcgcattcc ccagtcgccc tgcccacggc
     6241 accattggga aacagccata ctgaaaagct ctgcgctgcg gctgccattg gcgtaatact
     6301 tcagaaccaa atgcctttaa cgaggttatc ctggcaccat catgcggata aatctgcagg
     6361 tgaatattgc ctttttccag cgtcactaac tctcctacag gcccaggcca tggcgcgtca
     6421 aacgcctgtt gtagcgccag gtaatgctcg ttttttcgtt cagttttcat gagattgttc
     6481 cctgatgtag cggtaagaag cggtttcttg ttcatcggtt ggctcaatca gccagacgca
     6541 aacaaaggaa agaactgaga cagcaaataa aaacagagcg acataccacg gttttcctcc
     6601 ccctagcgcc agcaaacttg aggcgataag ggggcttagt ccgccaagaa tcgcggaaaa
     6661 ttgataagca aaagaaaggc cggtatatcg tactttggta ccgaacatgc gggtgaaaag
     6721 tgttggctgc acggcaaaca tcatcgtcgg acctaagttg tagccaagga tcatactgca
     6781 ccagatgatg agcgtacttt tgctatccag taataagaag aaaggaaacg cgaacaggac
     6841 acaaaaccct gctcccgaca aatacagact tttttgacct atacgatctg ataaccatcc
     6901 aaccaatgga caactgaaaa taccgatcgc cgaggcaatc aacatacccg tcaggggaat
     6961 atctcgcgac aatgctaatt ggctggaaat atagacaata ccaaaggcgt taataatatt
     7021 agaggagaca ctttccgcca gcctcgcacc cagtgccaat aaaatatttc gtggatgttt
     7081 tttgaataat tcaatcaacg gaggaacgga cttttcttta ttattcgtcg ttttttgctt
     7141 ttgaaaatcc agcgtttctt cggtatgcag gcggataaac agcccaacga tcaacatcac
     7201 cgcagaaagc caaaacggaa tgcgccatcc ccaggagaga aactgctccg aagtaagaaa
     7261 atggttacag agagcaaaaa taccggttgc cagcataata cccacagacg cagccgtttg
     7321 cgggagtgaa cctaaaaatc cgcgtcggga ttcgggcgct gattctatgg tcatcaatgc
     7381 cgctccgccg tactcgccac caagaccaaa gccctgaatt aaacgtagca ccattaatat
     7441 gagcggtgcc cagatcccaa tttcctgata cgtcggaata aaaccaatca aaaatgtgga
     7501 gcaaccgaca atagccaacg tccagataag cgttattttt cgccctattt tatcgccaaa
     7561 atgaccaaag acgatgccac ctaaaggacg agtcagaaaa ccgacaccaa aggtcgcaaa
     7621 ggaagctata agacctataa aaggatcgct gtctggataa aacagcggtg caaataccag
     7681 tcctgctgcc gtgccaaaaa taaagaagtc ataccattcc attaacgcgc ctgcgaaact
     7741 gcccataacg acacgtctca tggctttacc ggaaagccct tcacgcggtg cgggtaactt
     7801 ttcagacata attttcctct cctggtaggc gagaaatctg ggatacgaac cactaaacgt
     7861 gggcagtttg aattaatgcg catcggctta aaccgatgcg cattaaatta tttcttcaat
     7921 tcagccagaa cctctgcgtc ctcagcatca acatttgcac ccagtggttc ccaattaatc
     7981 aatacacagg tcaggaatcc caggaatggc acgattgcca accagtaaaa cgcttcggtt
     8041 ttcaacgcac cccacagcat cggccagaaa atgagtcctg caattgcccc tgtgcgcata
     8101 atagcgcggg caaatccgac cccagtaggg cgaatagcgg gaggataaga gagtgtggca
     8161 atggtcatgc ccagcccacc aggacccgct gaatggaaga aaataattga tcccaataac
     8221 cctaaagaca gccagggatt agtcgttgcc agtgcgccta acgacagcaa tgctaaggtc
     8281 acaacagcaa atccatacat cgactgtcgg cgagtaccca gtcgttggag aataaacgag
     8341 ccgatcatcc cgcctgtcac cccacaaagg gcattcacga cggcagaacc ggtcaacgcg
     8401 ccagtaagcc cgccacttat tattcccgcc aacgtaagag gaagataaac acctacggcg
     8461 ttatattgcc acgcctgcat ggttgccacg acacagccaa gaatggtgcg tttgcggtaa
     8521 cgatcattaa ataagatgcc atatccacca ctgtatttat tctctgcttt ttccgagaat
     8581 ttcgcttctg ttgtacccga tgccgcaacg cgagcctgaa caccataacg ttttcccaga
     8641 atgtcgcacg cttcctggta acgcccgacg cgtgcggccc acattgcgga ttcaccaata
     8701 aagaagtaac gcaaaatcat gaagatgact gcaaaaatag ctccgatgaa tattccgtaa
     8761 cgccacaatt gttcttcagc gatacccgac agaagcagcg gcaaaagcac caaataaaca
     8821 acggttgtcg aaacatacca ggccatttgc cagaggttga ccgacgttcc ggttttcttc
     8881 gacgttgaac cacgaagctc tgccacggca ttggttgcca aagggaagtc gatgcctaaa
     8941 ccaaagccca tgatgacgcg gcacaccagc acccaccaga tactgggagc aatagctacc
     9001 gctgcagcac cgatggtgca cagccccatg ccaataataa atgcccgctt ttgcccaaag
     9061 cggtttatta ctcggttcgc taataaaccg ccaattaacg cggcgattaa tactgatgca
     9121 ttgacggttg cagcaagacc cggcgaaatg ccgaattgag cggcaatgta tttattacca
     9181 aaccccacca tcgccgcctg gtaggcatca atcaatatgc cgcccaaagc aataaaaata
     9241 acgccgatac tggtgttgat tgcagagttc ttattaacaa tatcaattac gtcctgtggc
     9301 cgactaattg aataagttgc aggactggtg atttgactca tcgcagtgtc ctttttaatt
     9361 gttgtagggt ggcgctgacg tgacgcagcg ccagacagag atttagaaaa tcattccggc
     9421 gcaaattgca gcatgacctt tatggagtca ttttgctgcg cgcgttcgaa tgccgccgct
     9481 gcatcctcaa tggcaaataa atcggtaacc agttcactgg tatcaaaccg cccatccgcc
     9541 agccattcga taacagcatc gaaatcttca gggatgtaca tgccagagtt gagcaagtca
     9601 cgctcgaaac gttgcatcat cggtaatggt atttcacgcg gcccacttgg tacgcccatg
     9661 cacacgacgg tgcctccggc atatacacgg gtacatgcat cgttaagcgt ggcctgcgct
     9721 gcaacgacat caatcgcccc cgtaaatcgc acatctgcgg ccagctcacc tggtgcccaa
     9781 acttctgctg cgcccagttt taacgccagc gcgcgtttgg cagcgtcagg ttcaatcacg
     9841 gtaatcttac ctgcccccat aatgcgcagt gcctgcacga tggataagcc tatcgttccg
     9901 gcaccaatca ccagtacgct ttccagagaa gctttcggca ttcggttaac gcagtgacga
     9961 gcacaagccg ccggttcggc aaaggccaac acttttagcg gtaaagaggc tggtgcgaca
    10021 tggcaattac gcgcaggaac aatgtgttgt gagcgagcaa agcccggtgc gcgaaaacca
    10081 gcaacctgcg gtggttcaca aagattaaaa cgtcctgctt tgcaggctcg gcattccatg
    10141 caagccatga tgggatcgac cacaacatga tcgcccggct gtacattttt aacgtcgctg
    10201 cccacttccg taacgcgcgc tgcaatttca tgaccgggga cgacaggcgg tttggcaaac
    10261 ggatgaccgc ctttcagaac atgcagatcc gaaccgcata tcccgtagaa cacgggcgca
    10321 atccgtactt cgtgcggttt aagttgctgg tgtggtactt caacatattg agtggtgact
    10381 ttcccaatat ctgaaaataa aacctgggta attttatcca ttgttattct ccagaatcga
    10441 tatccatagg ggattagcgt tgcgcgatac cttcatcgac aatttcagcc agccgttgca
    10501 agcgtggaac ggagacatcg cgcagcatgg tttgtggatc ttgtgcacca acaaccgacg
    10561 cccaaaccgc tcgtccagcc aggaagcccg acgcaccacc tttcatggca atacttactg
    10621 cacggccaaa aatatcggca tcaacgccag aagaaagaat tacccacggc atcttcatct
    10681 gatcgttaag ttgctgacag gcagcaagta aggttttttc atcgcctttg ccccctaatg
    10741 gcatctcggc tttgtaaaga tcagcctctg ttccaccaag ctctgcggcg gcggcaacaa
    10801 tggcgctttc acgatcaaag tcccaaccgc gacgaggtgg acgcaccaca ggttcaataa
    10861 tgctaaccag cccggcacta cggcagcgac gaacaaattt atcaaccatt gccagacgtt
    10921 cctctgcggg ttcatcttca cgccagagta caagtaattt cattgcctta gcccccattt
    10981 cacgggcttt atgcgggtcg acggacatat ctatttcaac gctatcaacc gggatgccat
    11041 tacctgggat aaaaagatcg gcagcgacaa tcaggccgca agaattagcc acagcgccct
    11101 gctcgactat ttgttcaaga caaaattgtt tatccgccag aacggcagaa gcataaggag
    11161 aaaggattcg ggtcgcggca actttaaaat cggtgagtac actgtcggcg atgggttttg
    11221 gctgccctgc tgccgcaaac atcaggcgca tggcctcacg ctgatcgaca gctaacatgg
    11281 cgaatccacc acctgggcgg gagatatctt tcagcgtaaa tggggtgaag tgggatgaca
    11341 tagaaactcc tgttaataga taaaattaaa gaccagcctg ctggcgaacc tgatttagca
    11401 gggcgctcca gtcctgccgc cctttgccgt cttgacttgc tgcggcgtaa taactttctg
    11461 ctgcaatccc catattgagc ggcgcccccg ttttccgagc gacgtcaact gcaatacgca
    11521 gatctttcaa tgccaggtcc accatgaagg cgggagaaag atcgcctttc agcactttgc
    11581 caggccaggt ggtcgtaaaa tgtcctttcc ctgctgccgt accactcatc accttaattg
    11641 cgacatcaag atttaatccc agtgactcac ataacacagc ggcttcggag gacagcgcat
    11701 taagcgcaat gctcatatag ttgttaatta gcttcacgcg gatccccatt cctgggccac
    11761 cagcctcgac cagctcatta cccatgcaca tgagaatcgg gcgtgcacgc gcaatctgct
    11821 catcagtgcc gcccgctaaa atcagcaatg tgccatcaat cgcattcact gacgttctgc
    11881 ccacgggggc gtccatcata ttgatgcctt tttcttgtaa ttcccgtatt aatgcatcgg
    11941 tctgtaaggg atgaatagtc gacatatcaa tcactaacgc gtcagatgaa acagtttcac
    12001 agacgccttt ttcccccaac agcacctgtc gaacaatgtc cccattggga agcatagtga
    12061 tgataaattc agcgcttgct gccgcttccg ctggggtttg tgccgcagtg gccccctgcg
    12121 taactagcgc atcgacggcc tgtgtattga catcaaatac ctgtaatgaa tgcccttttt
    12181 gcaatagatt gttagccatt gggctaccca tctgccccag cccgagaaat gcaatggttg
    12241 ccatgaatac tctcctgtct gacattgaca aaataatgac aattgaaaat tcgttttcgt
    12301 ctgtttttga gcatatttgt aatttatgtt caaaaacacg acacgcatca ctaatttgat
    12361 catttgtgtc atttaaattg accaaagacg acaaaattaa acaggacaag acaaatatga
    12421 ccagaattgc atgcgtgggt atcacagttc aggacagaat ttattctttg cccactctgc
    12481 cggagggtgg cgggaaatat caggcaaatc attatcttga gattggtggc ggcccggcag
    12541 caacagccgc tgttgcaatc gcaaagcttg gcgtggaggt tgatttcatc ggtcgcgtcg
    12601 gcgatgacag ttgtggaaat accttacttg cagaacttga gggttggggc gtcaataccg
    12661 cattttgccg ccgatatcct aacgcccgtt cgtcgcaatc agcgatcctc gttgatcagc
    12721 atggcgaacg gattatcgtt aattatccaa gtcctgatct cggtactgat gccgaatggt
    12781 tagaagcgat cgatttctct cgttatgatc tgattctggc cgatgttcgc tggcatagcg
    12841 gtactgaaaa agcattctcg ctagcgcgcc ttgcgggtgt gacaacgctt ttagacgccg
    12901 atatgacacc gcaggatatt tctccacttg tcgcgcttgc cgatcacgcc gtgttctcaa
    12961 caccagggct gaaacgcatg actggcctgc agtcacctga agaaggtttg ttccaggcaa
    13021 cgacacaaac agcaggtaaa gtgtatgtta cgctgggttc tgaaggatcg ttgtggattg
    13081 aagatggtca tttgtgtcaa caggaggcat tttctgtcaa tgtggttgat accactggag
    13141 caggcgatgt ttttcacggc gcactcgcgg tagcgttggc agaaaaaatg ccaacaaaag
    13201 aggctattcg ttttgccagt gcggttgccg cgatgaaatg tacccagcct ggcggtcggg
    13261 ccggcattcc taatcgtgag caaaccgaat catttttgtc actttatgcg taaaatggta
    13321 aacatctacc gaggaggggc aagggagtat tcatgagtat catcgaagtg acaggtaatc
    13381 cacgccatga ccaactcgtt catctaatcg cagaacgcgg ttatatgaat attgaagaac
    13441 tggcacaact gctggatgtt tcaacgcaaa ccgtgcgccg ggatatccga aaactcagtg
    13501 agcagggctt gatcacccgt catcatggag gggccgggcg cgtctccagc gtcatgaata
    13561 ccgcttttga gcaacgggaa ctttcgctca ccgccgagaa acgggcgatc gctgaggcag
    13621 tcgccgatta ccttcccgaa cgctgtaccg tctttatcac catcggtaca accgtagaag
    13681 ccgttgccag ggcattactc aaccggcgtg atttacgcat tatcactaat agcctgcgtg
    13741 tggcacagat tctttataag aatcaggata ttgaagtgat ggtgccggga ggcactttac
    13801 gcgctcataa cggtgggatt atcggcccag gagccgtgga ttttattgaa ggcttccgcg
    13861 cagattattt aatcaccagt ataggtgcca ttgaacatga cggcacccta ctggaatttg
    13921 atgtcaatga agcgttagtc gcgagaacga tgattaaaca tgcgcggaat acattgttag
    13981 tggccgatca tacgaagttt accgcgtctg ctgccgtttc aattggcaat gcacggaatg
    14041 tcagggcttt ttttactgat gccccgcctc ccaattcttt ctgccagttg ttaagtgaag
    14101 agaatgttga actggtggtt gccgagcaag aagtatcctg aaattgaaac ttcggaaaat
    14161 ccgctgggaa tttcagcgga ttttgttttc cataagccaa aaaaaaacct gccatcgctg
    14221 gcaggttttt tatgactgaa aaacgaaatt aatcgtcttt cggtgcgcgg ttagcgcgac
    14281 gacgatcgtt ttccgtcagg tgacgtttac gaatacggat agaggtcgga gtcacttcta
    14341 ccagttcgtc atcatcgatg aactccagag cttgttccag agtcatgcgg ataggcggaa
    14401 ccagaacaac ggcttcgtca gtaccggaag cacgcatgtt ggtcagtttc ttaccggtca
    14461 ggcagtttac agtcaggtcg ttagagcggc tgtgaatacc gataatctga ccttcgtaaa
    14521 cttctgcacc gtgaccgagg aacagcttac cgcggtcctg cagaccgaac agtgcgaacg
    14581 cgaccgcttt accctgaccg ttagagatca gtacgccgtt ctgacgctgg cccacttcac
    14641 ccggacgtac gtcgtcgtag tggctgaagg tggagtacag cagaccagta ccggaagtca
    14701 tggtcatgaa ctcagaacgg aagccaatca gaccgcggct tgggatcacg tagtcgagac
    14761 gtacacggcc tttaccgtct ggattcatgt ttttcaggtc gcctttacgt tcgcccagcg
    14821 cctgcattac agaaccctga tgctgttctt caacgtccag agtcacgttt tcatacggct
    14881 cttgtttacg accgtcgatt tcacggaaga taactttcgg acgggatacc gccagttcga
    14941 aaccttcacg acgcatgttt tcgatcagaa cagacaggtg cagttcgcca cgaccagaaa
    15001 cgcggaacgc atcggcgtct tcggtttctt ctacgcgcag cgcaacgttg tgtaccagtt
    15061 ctttgttcag acgatccagg atctgacgag acgttacgaa cttaccttct ttaccgcaga
    15121 acggcgaggt gttaacgcag aagaacatag aaacggtcgg ctcatcaacg gagagtgccg
    15181 gcagcgcttc aacgttttgc gtgtcgcaaa cggtgtcaga aatgttcagt tcgcccaggc
    15241 ccgtgatcgc cacgatatcg ccagcttccg ccagatcggt ttcgatacgt tccagaccga
    15301 ggtggcccag cactttaccg actttcgcgt tgcgggtttt gccttcgcta tcgatgatag
    15361 taacctgttg gttcggcttc actttaccgc gcttgatgcg gccaatgccg ataacgccaa
    15421 catagctgtt gtaatcgagc tgagaaatct gcatctggaa cggaccgtca aggtcaacgt
    15481 ccggcgcagg aacgtggtca acaatcgcct ggtacagcgg ggtcatgtct tccgccatat
    15541 cttcgtggtc cagacccgcg ataccgttca gcgcagaagc gtaaacgatc gggaagtcca
    15601 gctgctcgtc ggtcgcgtcg aggttaacga acagatcgaa tacctgatcc acaacccaat
    15661 caggacgcgc gccagggcgg tcaactttgt tgataacaac aatcggcttc aggccgtaag
    15721 caaacgcttt tttggttacg aagcgcgttt gcggcatcgg gccgtcaaat gcgtcaacca
    15781 ccagcagcac tgagtctacc atggacatta cacgttcaac ttcaccaccg aagtcggcgt
    15841 gccccggggt atcaacgatg ttgatacggt aatcattcca tttgatagcg gtgtttttcg
    15901 cgaggatggt aatcccacgc tctttctcca aatcgttgga gtccatcacg cgctcttggg
    15961 tttcggcacg agagtcgaac gtaccggatt gttggagcag cttgtctacc agggtggttt
    16021 taccatggtc tacgtgcgcg atgatggcga tattacgcaa tttttcgatc acaactttgc
    16081 ctcaggcatt agaaatagcg cgttattgta cacggattaa tcgcactaca aaacaggatc
    16141 acaaacatcc tccgcaaaca agtattgcag agtccctttg tgatcgcttt cacggagcat
    16201 aaaaagggtt atccaaaggt cattgcacca atatggtgct taatgttccc attgaagcac
    16261 tatattggtg caacattcac atcatggtgc agcccttttg cacgatgtta agcatgataa
    16321 cgccttttag gggcaattta aaagttggca cagttttcgc tttatctttt ttacggcgac
    16381 acggcccatt taattgcaga tttcgttacc acgacgacaa tgaccaatcc aggagagtta
    16441 aagtatgtcc gctgaacacg tactgacgat gctgaacgag cacgaagtga agtttgttga
    16501 cttgcgcttc accgatacca aaggtaaaga acagcacgtc actatccctg ctcatcaggt
    16561 gaatgccgaa ttcttcgaag aaggcaaaat gtttgacggc tcctcgattg gcggctggaa
    16621 aggcattaac gaatccgaca tggtgctgat gccagacgca tccaccgcag tgattgaccc
    16681 gttcttcgcc gattctaccc tgattatccg ttgcgacatc cttgaacctg gcaccctgca
    16741 aggctatgac cgtgacccgc gctctattgc gaagcgcgct gaagattacc tgcgttctac
    16801 tggcattgcc gacaccgtac tgttcgggcc agaacctgaa ttcttcctgt tcgatgacat
    16861 ccgtttcgga tcgtctatct ccggttccca cgttgctatc gatgatatcg aaggtgcatg
    16921 gaactcctcc acccaatatg aaggtggtaa caaaggtcac cgtccggcag tgaaaggcgg
    16981 ttacttcccg gttccgccgg tagactcagc tcaggatatc cgttctgaaa tgtgcctggt
    17041 gatggaacag atgggtctgg tggttgaagc tcatcaccac gaagtcgcta ccgcaggtca
    17101 gaacgaagtg gctacccgct tcaataccat gaccaaaaaa gctgacgaaa ttcagatcta
    17161 caaatatgtc gtgcacaacg tggcgcaccg tttcggtaag accgcgacct ttatgccaaa
    17221 accgatgttc ggtgataacg gttccggtat gcactgccac atgtcgctgt ctaaaaacgg
    17281 cgtcaacctg tttgctggcg acaaatacgc aggtctgtct gaacaggcgc tgtactacat
    17341 tggcggcgta atcaaacacg ctaaagcgat taacgccctg gcaaacccga ccaccaactc
    17401 ttacaagcgt ctggtcccgg gctacgaagc accggtaatg ctggcttact ctgcgcgtaa
    17461 ccgttctgcg tctatccgta ttccggtggt ttcttctccg aaagcacgtc gtatcgaagt
    17521 acgtttcccg gacccggcgg ctaacccgta cctgtgcttt gctgccctgc tgatggccgg
    17581 tcttgatggc atcaagaaca agatccatcc gggcgaagcc atggacaaaa acctgtatga
    17641 cctgccgcca gaagaagcga aagagatccc acaggttgca ggctctctgg aagaagcact
    17701 gaacgaactg gatctggatc gcgagttcct gaaagccggt ggcgtgttca cggatgaagc
    17761 gatcgatgcg tacatcgctc tgcgtcgcga agaagatgac cgcgtgcgta tgactccgca
    17821 tccggtagag tttgagctgt actacagcgt ctaatagttg aagttgtact acccggcgca
    17881 acaacgccgg gatttagttg ccgtggaaac tttcagccca tctctgcatg ggcttttttc
    17941 tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat
    18001 ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag
    18061 atccttaact cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac
    18121 gccaaccccg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacaccg
    18181 ttaccggaac tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag
    18241 gcggggcaag gttttaccga taacgaagtg acgctggtca tcgacgggcg ctcgcatatc
    18301 ctttctgtga cggctcagcg tatgccggac ggcatgatcc tgctggagat ggcaccgatg
    18361 gataaccagc gccgcttaag tcaggaacag ctacagcacg cccagcaggt tgctgcccgt
    18421 gatttagtgc gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg
    18481 gcgcagctgc tcagcaaagc attacctgat ccgtcactac tcgaatatac caaagtgatt
    18541 atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc
    18601 ggtacgcgca ttaccgaaag tattcacaaa gtggctgaac gcgtggtgac gctggtgtcg
    18661 atggaactgc cgaacaacgt gcggttgatt cgggattacg accccagcct gccggaactg
    18721 gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag
    18781 gcgctggggc cggagggtgg tgaaatcatt ctacgtaccc gcacagcgtt tcaactgacc
    18841 ttacacggcg agcgctatcg gctggcggcg cggattgatg tggaagataa cgggccgggc
    18901 attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc
    18961 accgggcttg gcttatccat cgcccgtaat ttgattgatc agcattcagg caaaattgaa
    19021 tttaccagtt ggccaggtca taccgagttc tcggtttacc tgcctatcag gaaataaagg
    19081 tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg
    19141 tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac gacatttgag aacggcgcgg
    19201 aagtactgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc
    19261 cgggaatgga cgggctggcg ctgctcaagc agattaaaca gcgccatccg atgcttccgg
    19321 tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg
    19381 catttgatta tctgcccaaa ccgtttgata tcgacgaagc cgtggcgctg gttgagcgcg
    19441 ccatcagtca ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga
    19501 ccgatatcat cggtgaagcg ccagccatgc aggacgtgtt ccggattatc ggtcggcttt
    19561 cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg
    19621 ctcatgccct gcatcgccac agtccgcgag ccaaagcgcc gtttatcgcg ctcaatatgg
    19681 cggctatccc gaaggatttg atcgaatccg aactgtttgg tcacgagaaa ggcgcattta
    19741 ccggcgcgaa taccattcgt caggggcgtt ttgaacaggc tgatggcggc acgttatttc
    19801 tcgatgaaat tggcgatatg ccgctagatg tgcagacgcg tttgctgcgc gtgctggcag
    19861 acggtcagtt ttatcgcgtt ggcggctatg cgccggtgaa agtagatgtg cggattatcg
    19921 ctgccactca ccagaatctt gaacagcggg tgcaggaagg caagttccgt gaggatctgt
    19981 tccaccgcct gaacgtcatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata
    20041 ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg agagctgggc gtagaagcga
    20101 agttgctgca tccggaaacc gaagccgcgc tgacgcgtct ggcgtggcca ggcaacgtgc
    20161 gccagctgga aaacacctgt cgctggctaa cggtgatggc tgccgggcag gaagtgttga
    20221 ttcaggattt gcctggcgaa ctgtttgaat caaacgtacc ggaaagcact tcacacatgc
    20281 agccggacag ttgggcaacg ctgttagcac agtgggcaga cagagcgctg cgttccggtc
    20341 atcaaaacct gctttccgaa gcacagccag agctggaacg gacgttactg accaccgcgc
    20401 tgcgacatac gcaggggcat aaacaggaag cggcgcggct acttggctgg ggtcgcaaca
    20461 ccctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcttgt gtgtaaagat
    20521 tgattattga gcgcaaattg ctggtatttt gcgctttact gttccgataa gttcagtatg
    20581 atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga
    20641 gtggcgcagt tgacagccac acaccgcaaa ctgctgttgc tgccgtgctg tgtgccgcga
    20701 tgatagggct gtttagctga agctcgttgt ttacgccgga tgcggagaaa gtcgtaggcc
    20761 tgataagacg caaccagcgt cgcatcaggc gttgattgcc ggatgcggcg taaacgcctt
    20821 atccggccta caaaagcaat aatttcaaca gattttgcag gcctgataag cgtagcgcat
    20881 caggcaatct tgttctcagc acattgatgg cgctttcgtt ttctactttg taaacgaagc
    20941 gccattcaca atttaaatca cccgagagaa ctgctgcatc cgcgctttct ggcgcagata
    21001 ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga cctttcgccg tcacctgtat
    21061 tcccttctca tccacatcca ccagcccatc ttttgctaac ggggcgagca gcttgagatc
    21121 ttccgcaaag taatcagcga agagcaaatc ccactgttgc tcaatagggg cgtaatccag
    21181 acggaagttg cagatgagcg acttaatcac atcgcggcga atacagtcat cacgcgttag
    21241 cgcaatacca cgccacagcg cattgccttg ttcatccact tgctgatagt actgcttcaa
    21301 ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa cgcccatccc
    21361 cagcagatcg gtatcgccct gagtggtgta gccctggaag ttacgatgca gcacgccttc
    21421 acgctgggcc accgccagct cgtcatccgg acgggcaaag tgatccatac cgataaactg
    21481 atagcccgat tgtgtcagga aggcgatggt ttcctgcagg atatcgagtt tttgctgcgg
    21541 actcggcagg tcagcatctt tgattttgcg ctgggcggca aaaatggtcg gcaaatgcgc
    21601 gtagttaaag acgctcagac ggtcagggtt cagttccgcc acacgcttca gggtaaaggc
    21661 gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg tcaatgttgg tggaggtaaa
    21721 gccaatctca cgcgcatggt taagcagtgc aaagatgaac tcttcatcct gctcgcggtt
    21781 aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc atgctcagcc gattaaagcc
    21841 ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc cgcggatcga cttcgatcga
    21901 gatctccgca tcggcattga actggaagtt ttcgcgcagc agcttcatca gacggctgat
    21961 ttgcgcttta ttcagatacg tcggcgttcc gccgccccag tgcaattggc tgacgtgacg
    22021 cccggcgaac agcggtgcac gatggacgat ttcttgctcc agcgcgtcca gatactgatc
    22081 ggccttgtgc tgctggcgag taacaatctt attgcaaccg cagaagtaac aaagcttatg
    22141 gcagaacgga atatgtacat agagagataa tggacgctca ggatagcgcg ccacagcttg
    22201 taaaaacgcc tgttcgccga agtcttctga aaactccagc gcggtcgggt acgaggtgta
    22261 tcgtggcccg gaatagttat atttctggat cagggccaga tcccagtcga tttgctgtac
    22321 agacatgctc actccttccg atggcgtctc tgacggctac ggcgtaccgg cggatgcccg
    22381 tttcgagtca tcagccggtt gcgcagccac ttttgtcgcc gcgacaaccg ctgtagttta
    22441 acgaataacc acaccagata acatataacc ggaagggtta tcagcagaac gggaaggccc
    22501 acggcggcaa tccgttagtt gccccgcagc agacgcatca tgtcttcttg cttctcgtct
    22561 tcttcctctt cttcgtcatc gtcataagag aggccgagtt tctgcatcaa ctcatcaata
    22621 cgatccagtt tggcatccac ccaggattgc tcttcggcgc tcagggtttc gcctgcttcc
    22681 agacgttcca gcagcgcatc cagacgctca tccgtttcca gtaactccaa ctccgcctgc
    22741 ggtgaaagca taggtttctc actcttcggt ttgtgctgtt tggtgacttt ttcagtcacg
    22801 cccaatggaa taggggtttt actgccaata cgtggatctt ttggtgcgtt ctgacctttg
    22861 ctgcctgacg tggtgttacc gcccgctgcg cggctgcccg gcgcatgacc acgacgtttt
    22921 ttctgacgct tacggtcacg agcttcctga tccagctcct cgcgtgtttt tcgacgcgct
    22981 tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt tcataatggt tcgtctttta
    23041 gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca aagaaaaaag
    23101 gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg tcttacaatc
    23161 cttgcaggcc gccaacacac cctgtttttc cattagccgg aacaatccgt gttgattcca
    23221 tttccattta attacgtctc cggacgttta tcttcctgac aatcctctgt cttcgcctcc
    23281 tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc tctatgggtg
    23341 ctactttacg cctttgcttt aaataaacaa tcaaccgcca tcagtatttg cacattttca
    23401 gagttttata taaaagcaat tcactgattt atatagctta tttgacatac gtcgcagaat
    23461 tttctctgct atttcgcata aagactatcg gcaatgtctc acaaagcata tggcttttac
    23521 ttacaacgct cacgccgaaa aatccgcctt ttattatggt tcaggtataa tcgccgcaaa
    23581 gctttgactg atggagacga ccgctttgac taatttgaat tatcaacaga cgcattttgt
    23641 gatgagtgcg cctgatattc gccacctacc ttccgatacc ggaattgaag tggcttttgc
    23701 aggccgttcc aacgcaggta aatccagcgc gctgaacacg ctgactaacc agaaaagcct
    23761 ggctcgtacc tcaaaaaccc cagggcgcac ccagcttatc aacctgtttg aagtggctga
    23821 cggcaagcgt ctggttgact tgcctgggta cggttatgcg gaagtcccgg aagagatgaa
    23881 gcgcaaatgg cagcgcgcgc tcggcgagta cctcgaaaaa cgtcagagcc tgcaaggtct
    23941 ggtggtgcta atggatattc gccatccgct gaaagatttg gatcagcaga tgattgagtg
    24001 ggcggtagac agcaatatcg ccgtgctggt actgctgacc aaagcggaca aactggcaag
    24061 cggcgcacgt aaagcgcaat tgaatatggt gcgtgaagcg gtgctggcgt ttaacggtga
    24121 tgtgcaggtt gaaacgtttt cttcgctgaa gaaacaaggc gtggacaagc tgcggcagaa
    24181 actggatacc tggtttagcg agatgcagcc tgtagaagaa acgcaggacg gcgaataatt
    24241 ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc ccgtaaattc aatagattac
    24301 aattctaccg ttggcctgat aaacagggcg catcaggcat tacggatctt ttctttcgcc
    24361 caataaaaaa cgccccagtc attactgact ggggcggcta aaatattcag ccaaatccga
    24421 ttacgtgaag taaaaggtct gaaagataga acatcttacc tctgtaccct acgcgactaa
    24481 ctttactctt ttttgttcag ttcagaaagc attttttgta attttttttc agttgttgca
    24541 taggaatttc taatagccat cacaaaacgt gacagcttat gttgcttact tacgaaaaaa
    24601 gggcatgttc aggcgaatct tagtgcgcct gatcccagtt ttcgccactc cccacttcca
    24661 ccagcaacgg cacatccaga cgggtacagt tttccatcag ttgatgaatc tgcttcgcga
    24721 cggcatcgac atcatcttta tggacttcaa ataccagttc atcgtgtacc tgcatgatca
    24781 tacgcacacg cggttgctca gcctgtagcc acgcatcaac ggcaatcatc gcccgtttga
    24841 taatgtcggc ggcggttccc tgcattggcg cgttaatggc tgcacgttca gccgctgcac
    24901 gacgagcccc attgctggat ttgatatccg gcagatacag acggcgtccg tccagcgttt
    24961 caacgtagcc ctgctctttc gcctgagcac gggtgcgttc catatactcc agcacgccag
    25021 ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc tttacgtgga atgttcaatt
    25081 gccgcgccag accgaaagca ctcatgccat aaatcagacc aaagttgatc gctttcgcgc
    25141 tacgacgttg ctcgctggtg acggtttcca gtggcaaacc aaacacttct gccgccgttg
    25201 cacggtggat atcttttcct tccgcgaatg cggtcagcaa gcctttgtca cgcgagagat
    25261 gcgccataat gcgcagttca atctgcgagt agtccgctga gacaatcaca taatcctctg
    25321 gcgcaataaa cgcctggcgg atacggcgac cttcttcgtt acgcaccgga atgttttgca
    25381 ggttaggatc ggttgacgat aaacgtcccg ttgcggttac tgcctggtga taagaggtat
    25441 gtacacgccc ggttttcggg ttgatcatca acggcagctt gtcggtgtag gtcgatttca
    25501 gcttcgccag accacgatac tccagaatca cttttggcaa cggatagtcc agcgccagtt
    25561 cttccagtac ctcttccgac gttgacggcg cgccacccgg agttttcttc agcggtttaa
    25621 tgccctgttt ttcaaacaga atggtttgta actgcttggt ggaagaaagg ttaaattctt
    25681 cacctgcaat ttcatgcgct ttcttttcca gctcagccag acgcaaggtg agctcttcag
    25741 agtgattgtg cagcactttc ggatcgatct tcacaccgtt acgttcaatg cgtgaaagca
    25801 ccggcaccag cggcatttcg atattctcga agacgttcaa cggccctttg tgtttttgca
    25861 gatccggcca cattttcaga tgcaactgca aggtgacatc tgcatcttcg gcggcgtaac
    25921 gtccggcttc ttcgagggca atctggttaa aggtcagttg attttttcct ttaccagcaa
    25981 tctcttcaaa agtgatggtt ttgtgcttca accaacgttc cgcgaggctg tccatatcgt
    26041 gacgcccggc aacgctattg agaatgtagg actccagcat ggtatcaaac gcaatcccac
    26101 gcagctcaat gccgtaattc gccagaatac cgcgatcgta tttcaggttt tgcccgacct
    26161 tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc gagtgcacgc tcgcgagaga
    26221 tttgatcggg cgcatcaaga taatcatgag caaccggaat atatgccgct acgcctggct
    26281 cgatagcaaa agaaagcccg accaggttag cagagatgtt atcaaggctg tcggtttcgg
    26341 tatcaaatgc aaataccggc gctttttcca gcttcgcaat ccacgctttc agtgtttctt
    26401 catcaaggat ggtgacgtag ttgtcataag aaatcaccgt tgccgtcact tctggcgctt
    26461 cgtctgcaac actggtttcc tgcggcctgg ctgcgggttt cgccccttta gcctgtagcc
    26521 atttgcccgc ttcgacatca gcagtccagc gtttgaactc atactttttg aacagcccta
    26581 acaactcttc cgccgccggt ggctgcactt ccagttgttc acaggtcagc tccagttcaa
    26641 cgtcggtttt aatcgtcgcc agctggtatg agagataagc aacttctttg ttttgctcga
    26701 gcttggctgc cattgttttc gcgccacgga agctcaaccc agcaattttt tctggctcgg
    26761 catacagcgt atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt ttttcaccga
    26821 cgcccggtac gccagggatg ttatcggagg agtcacccat cagcgccagg aaatcgatga
    26881 tcagttctgg cggcacgccg tacttattca ccacctcttc cggtccgagg atggtattcg
    26941 tcatggtgtt gataagcgta atatttggcg tcaccagctg cgccatatct ttatcgccag
    27001 tgctgatcag caccggacgc ccggcttttt cggcttcgcg cgccagagta ccgataacgt
    27061 cgtccgcttc tacgccagaa accgccagca gcggcagtcc catcgcttta accatcgcgt
    27121 gcagaggttc gatttgtgct cgcagatcgt ccggcattgg cgggcgatgt gatttgtaat
    27181 gttcaaacag ttcatcacga aaggtttttc ccttggcgtc aaagaccacc gctgcatgcg
    27241 tcggtttata ttgcatgatc agactgcgca gcatgttgag gacaccatac atcgcaccgg
    27301 tcggctcgcc tgcgctgtta gtcagcgggg gaaacgcgtg atatgcgcga taaagataag
    27361 atgaaccatc tacaaggata agtggatttt gggggatctg aaccataatg tccgtgcctg
    27421 tttatcagat tatgggtaaa ggatgccaca gacaggatga aaacatgagt ttttcaccgc
    27481 ttttcgtgaa aatcttttgt agatcttctg gatcgctcgc aaatcgtcat gtggataact
    27541 ttgtgcataa attatttaca tcgcttttaa tttattatca tgctttttac agcatgatgt
    27601 ttgtctttga ttttcatgca gttaaaaata aagccatccc taataattga gataatggga
    27661 ttttttctaa tgtggataga tatgaattat ttttctcctt aaggatcatc cgttatttgg
    27721 gtcgttttct gctaaaatct gcgccttgcg cttctttagc gcactgtcta tggctaacct
    27781 tttgaataaa ttcattatga cgagaatact cgctgcgata acccttttgc tgagtatcgt
    27841 attgaccatt ttggtcacta ttttttgctc cgtcccgatc atcattgccg ggattgtaaa
    27901 actattgctt cctgtgccag tcatttggcg aaaggtttcg cgtttttgtg attttatgat
    27961 gtattgctgg tgtgaaggtc tggcggtatt actgcatctg aacccacacc tgcaatggga
    28021 agttcgcggg ctggaagggt taagtaagaa aaactggtat ctgcttattt gtaaccaccg
    28081 tagttgggca gatattgtcg tactgtgcgt gctgtttcgt aagcacattc cgatgaataa
    28141 atatttcctc aagcagcagc tggcctgggt gccttttctt ggcctggcat gctgggcgct
    28201 ggatatgccg tttatgaagc gttattcccg cgcttattta ttacgtcatc cggagagacg
    28261 tggtaaagat gttgaaacca ctcggcgttc ttgtgaaaag tttcgcctgc atcccaccac
    28321 cattgttaat ttcgttgaag gctcccgttt cacgcaagaa aaacatcagc aaacgcactc
    28381 cacttttcaa aacttgttgc caccaaaggc ggcaggcatt gcgatggcgc taaatgtact
    28441 gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt tatccggaca ataaccgtca
    28501 gccgttcttc gatatgttaa gcggtaaact gacgcgaatt gtcgtacatg tggatttgca
    28561 gcctattgcc gatgagttac atggcgacta catcaatgac aaaagcttta agcgccattt
    28621 tcagcaatgg ctgaactcac tgtggcagga aaaagaccgg cttctgacgt cgttgatgtc
    28681 atcacagcgg caggacaagt aaattttgct cgcctctgaa tgagcagagg cgagtgagta
    28741 ttttaatgaa caaaacgtcc ggcacgagac ataaattctt cttcgctcat ctcctgtccg
    28801 ttaaaaacaa ctttacccgg tgtataacgc agttgcagcg aggcggtatt gttgtcaatc
    28861 gtgattaact gcaacatctg acctaaggtc atcatcccgc tgatttgttt atcagactgc
    28921 ttttgcgctt tttccgcatc cattccttca gataaattaa gctgttttgc ggtttctgtg
    28981 gcgacattaa gcggtaattt cacatcaaaa ttgagcgatt taatatcttt gtttgtggat
    29041 gatgaagatt tggctgggtc ggcaatactg atatccagat tggcatttag ttcgccgagt
    29101 gcattcttcc atcttaccgg ttgtttaatg gtcggctcac ttttttgtaa taacggtaaa
    29161 tactctttga acaatgcagt attcacttct tgcagagcaa cttcatcgtt tgctaactca
    29221 gggtgtgcag caagctgctt ctgcatcgca atgttatact ggatgataaa ttggcgcacc
    29281 gctgagggat caatagattc agcaatcaat gaccactcac cactaccgag ttgctgattc
    29341 ccttttgtta acttatcaag ttcgtaggtc agattgacat tgatataatc tttgtcttta
    29401 acgcgatcca gcttggtttt tgcaacgaaa gcatcgattt gggcaacgtc ttccccgtga
    29461 ttgataatgt taatctgatt aaatttcgac tcgctttcac caaccggaaa tttcttttct
    29521 tctaatcgcg ccagagaatc aatggtaaaa cttttcgccg ttactttgtt cattggtgat
    29581 aattgcagaa caatatcttc aactttgcct tcaatatcca ggttagacaa tgaactatca
    29641 tcaccattaa aagtaaactg gccttcacca aaagaaaact ttccatactc tgcaggggca
    29701 acatctaatg tcgtggagaa tgagccacca aacgcgaatc gcaatgtcgc ttccacgggc
    29761 gatttatttt tcgcggcgat aaatagcggt tgtgtcagct cattattcac taagttcact
    29821 tttgccgctg ccagtgctgg gatgacatta ccatgcatta acattgtgat gggcaacgga
    29881 ccgtgctcaa catccacatc aaatacaact ttctggcctg gcttgatatt gagatcgggt
    29941 gcaccgttat caaatgtcat ttgcatttga aaacgggaat taaaaaaacc tttgtcaaaa
    30001 tttttataac ttaacgtcat gtcgtaggca tgttcacctt tctttttagc atcgttaaaa
    30061 tctttaataa acttttcgac acctggctga atttgcgtac ctgtgtacca tgtgccacca
    30121 ccccagatta cggataacgc aacaatggca cctgtagctg acttacgtat catataaata
    30181 ttccctgata aaacagcaaa actttgaata tccacttatg ctgaaataat gtccagca
//
LOCUS       DAJOTB010000046        29952 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJOTB010000046 DAJOTB010000000
VERSION     DAJOTB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.046"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            259..474
                     /gene="essD"
                     /locus_tag="OHN28_002024"
     CDS             259..474
                     /gene="essD"
                     /locus_tag="OHN28_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2297423.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            479..793
                     /locus_tag="OHN28_002025"
     CDS             479..793
                     /locus_tag="OHN28_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCQ2297424.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(790..1029)
                     /locus_tag="OHN28_002026"
     CDS             complement(790..1029)
                     /locus_tag="OHN28_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297425.1"
                     /translation="MQKEVFINITADCSSPASTAKEIEALKYMITVIFSVLDQNEKNG
                     IIHQLNEHVNNPYIKSNLEMLLPMKDIGKPTETKG"
     gene            1164..1697
                     /locus_tag="OHN28_002027"
     CDS             1164..1697
                     /locus_tag="OHN28_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2297426.1"
                     /translation="MNVKIRYGLSAAVLALIAAGASAPQILDQFLDEKEGNHTKAYRD
                     GSGIWTICRGATVVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1914..2096
                     /gene="rzoD"
                     /locus_tag="OHN28_002028"
     CDS             1914..2096
                     /gene="rzoD"
                     /locus_tag="OHN28_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HCQ2297427.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2187..2480)
                     /gene="iss"
                     /locus_tag="OHN28_002029"
     CDS             complement(2187..2480)
                     /gene="iss"
                     /locus_tag="OHN28_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HCQ2297428.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            2961..3287
                     /locus_tag="OHN28_002030"
     CDS             2961..3287
                     /locus_tag="OHN28_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCQ2297429.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            3494..3676
                     /locus_tag="OHN28_002031"
     CDS             3494..3676
                     /locus_tag="OHN28_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297430.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            4240..4785
                     /locus_tag="OHN28_002032"
     CDS             4240..4785
                     /locus_tag="OHN28_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCQ2297431.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            4760..6685
                     /locus_tag="OHN28_002033"
     CDS             4760..6685
                     /locus_tag="OHN28_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCQ2297432.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQDLDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            6682..6888
                     /locus_tag="OHN28_002034"
     CDS             6682..6888
                     /locus_tag="OHN28_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCQ2297433.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            6885..8486
                     /locus_tag="OHN28_002035"
     CDS             6885..8486
                     /locus_tag="OHN28_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2297434.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIARRVVTSPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            8467..9798
                     /locus_tag="OHN28_002036"
     CDS             8467..9798
                     /locus_tag="OHN28_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCQ2297435.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAVLEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSSDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADITTVEPAK
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHRREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNLASDTNKELLNTPEALPV"
     gene            9808..10140
                     /locus_tag="OHN28_002037"
     CDS             9808..10140
                     /locus_tag="OHN28_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCQ2297436.1"
                     /translation="MATKEEFNHYQPLGNSDPAHTAIAPGGLSAKTPAMTPLMLDGTT
                     RKLVVWDGTTDGAAVGILAVAADQTSTTLTFYKSGSFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            10196..11221
                     /locus_tag="OHN28_002038"
     CDS             10196..11221
                     /locus_tag="OHN28_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCQ2297437.1"
                     /translation="MSVYTTAELLASTQHHFKFDPLFLRLFFRETYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIRQNMLDENLAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            11263..11658
                     /locus_tag="OHN28_002039"
     CDS             11263..11658
                     /locus_tag="OHN28_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCQ2297438.1"
                     /translation="MTKDELIARLRSLGEQLNRDISLTGTKEELALRVAELEEELDDT
                     GDTAGQDIPLSPENVLTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARIQ"
     gene            11670..12023
                     /locus_tag="OHN28_002040"
     CDS             11670..12023
                     /locus_tag="OHN28_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCQ2297439.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCY
                     LWLGRGAPPAVNRRR"
     gene            12034..12612
                     /locus_tag="OHN28_002041"
     CDS             12034..12612
                     /locus_tag="OHN28_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297440.1"
                     /translation="MVIRGLEQAVGNLSRISKKAVPGAAAMAINRVASSAISQSAIQV
                     ARETKVRRKLVKERARLKRATVKNPLARIRVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     HSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            12609..13004
                     /locus_tag="OHN28_002042"
     CDS             12609..13004
                     /locus_tag="OHN28_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297441.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            13012..13752
                     /locus_tag="OHN28_002043"
     CDS             13012..13752
                     /locus_tag="OHN28_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297442.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGRVNIPVVSGNGEFAAVAEITVTAS"
     gene            13768..14190
                     /locus_tag="OHN28_002044"
     CDS             13768..14190
                     /locus_tag="OHN28_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCQ2297443.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RQVTVEDVIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMSEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            14172..14606
                     /locus_tag="OHN28_002045"
     CDS             14172..14606
                     /locus_tag="OHN28_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCQ2297444.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFDPDGNEVIPASPDVTGMTEDDVMLMTVSEGIAGGVRYG"
     gene            14599..17166
                     /locus_tag="OHN28_002046"
     CDS             14599..17166
                     /locus_tag="OHN28_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2297445.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQEMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQTDYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGERQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQ
                     QADKFAQQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNSLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGVTAIQAAAAKFHFATGGF
                     TGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGGTGGPAQMRRS
                     EGIRFEQNNNVVIQNDGTNGLPGPQMLKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
     gene            17163..17492
                     /locus_tag="OHN28_002047"
     CDS             17163..17492
                     /locus_tag="OHN28_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297446.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVI"
     gene            17492..18190
                     /locus_tag="OHN28_002048"
     CDS             17492..18190
                     /locus_tag="OHN28_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2297447.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLASTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            18196..18939
                     /locus_tag="OHN28_002049"
     CDS             18196..18939
                     /locus_tag="OHN28_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297448.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            18876..19508
                     /locus_tag="OHN28_002050"
     CDS             18876..19508
                     /locus_tag="OHN28_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2297449.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPVFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQVVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            19569..23051
                     /locus_tag="OHN28_002051"
     CDS             19569..23051
                     /locus_tag="OHN28_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2297450.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHFRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRFGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWIYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQARTLTLD
                     REIMLPSSATTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSMARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLNFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITGVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVG
                     KAFPRNSSYASGTITVTVYDDQAFDRQIVIPPVLFRGGKHENFNSNNQQSYWYSTCKL
                     QVLKNGQEIFQQPATDVSRVFSSVIDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLL
                     VVVMKKSTAGISIS"
     gene            23110..25170
                     /locus_tag="OHN28_002052"
     CDS             23110..25170
                     /locus_tag="OHN28_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2297451.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSKPGTLNDFLGAATEDDVRPEA
                     LYRFEKMVEEVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEVAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            25167..25445
                     /locus_tag="OHN28_002053"
     CDS             25167..25445
                     /locus_tag="OHN28_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297452.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            25458..25751
                     /locus_tag="OHN28_002054"
     CDS             25458..25751
                     /locus_tag="OHN28_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297453.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLINRG"
     gene            complement(25843..26700)
                     /gene="sitD"
                     /locus_tag="OHN28_002055"
     CDS             complement(25843..26700)
                     /gene="sitD"
                     /locus_tag="OHN28_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCQ2297454.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(26697..27554)
                     /gene="sitC"
                     /locus_tag="OHN28_002056"
     CDS             complement(26697..27554)
                     /gene="sitC"
                     /locus_tag="OHN28_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCQ2297455.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQALLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(27551..28378)
                     /gene="sitB"
                     /locus_tag="OHN28_002057"
     CDS             complement(27551..28378)
                     /gene="sitB"
                     /locus_tag="OHN28_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCQ2297456.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(28378..29292)
                     /gene="sitA"
                     /locus_tag="OHN28_002058"
     CDS             complement(28378..29292)
                     /gene="sitA"
                     /locus_tag="OHN28_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCQ2297457.1"
                     /translation="MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         7369 a         7266 c         8677 g         6640 t
ORIGIN      
        1 gaacactcgg tggcattctc acaaatatca tttagtagtt tacgtacgta aaaaattggt
       61 tatgctgtta agagtggtta cttcgtcaca cagcttaaac ccgccgtcga gcgggttttt
      121 ccattttttg agtctcgata ttagctgata acccaatacc tgagttattc actgactccg
      181 aatctgttac gtttctgcct ttattgcgat acgtagtatc cccttaattt acacccgctt
      241 tgtctgcgag gtggggttat gaaatccatg gataagttaa caacgggtgt cgcctatggc
      301 acctcagcag gtagtgccgg gtactggttt ttacagttgc tcgataaagt cacgccctca
      361 cagtgggcgg caataggtgt gctgggtagc ctggtatttg gcctgctgac gtacctgaca
      421 aacctttatt tcaagattaa agaagataag cgtaaggctg cacggggaga gtaattcaat
      481 gactcaaaac tatgaactga ttgtgaaagg gatccgcaat tttgagaata aagttacggt
      541 aactttagcg ttacgggaca aaaaacgctt tgacggtgaa atttttgacc tggacatctc
      601 gctggaccgt gttgaaggtg ccgcgctgga gttttatgag gctgcagcca gaaggagcat
      661 cagacaggtc ttcctggatg ttgctgccgg gttatgtgaa ggggacgagc tgttgccaga
      721 aacgcgcccc tgttcagagg cgcggtatac cataaaaatt aacagttcag ataactcgat
      781 tactggttgt tagccttttg tttcagttgg ttttccaata tctttcattg gtagcagcat
      841 ttccagattg cttttgatat atggattatt tacatgttca tttaattgat gaatgattcc
      901 atttttttca ttctggtcca atacggaaaa tattactgta atcatatatt taagagcttc
      961 aatttctttt gctgttgatg caggagatga gcagtcagct gtaatattga taaaaacttc
     1021 tttttgcata tcaaatcctc agatggggta gctcagccag catcccccat aatccttaac
     1081 cagagcgcca gtgtcctacc actgacgggc tgaatgctta acatatccag ggttcagaaa
     1141 ccgataaatc ctgataaata tccatgaacg taaaaatcag atacggcctg tcggctgctg
     1201 ttctggcgct gattgccgca ggcgcgtctg ctcctcagat acttgaccag tttctggacg
     1261 aaaaagaggg taaccacaca aaggcatacc gcgatggttc cggcatatgg accatttgcc
     1321 ggggtgccac ggtggtggat ggtaaacctg ttattccggg catgaaactg tcgaaggaaa
     1381 aatgcgccca ggttaacgcc attgagcggg ataaggcgct ggcatgggtg gagcgtaata
     1441 taaaagttcc actgaccgag ccacaaaaag caggtatcgc gtcattttgc ccctataaca
     1501 ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gcttaatgcc ggtgatcgta
     1561 aaggtgcctg tgagtcgatt cgctggtgga ttaaggacgg tgggcgtgat tgccgcacac
     1621 gttcaaataa ctgctacgga caggttattc gtcgtgacca ggaaagcgca ttagcctgtt
     1681 gggggataga tcagtgagaa gagttgccgc gattatctcc gctctggtta tctgcatcat
     1741 cgtctgcctg tcttgggctg ttaatcatta ccgcgataac gcgattacct acaaagccca
     1801 gcgcgacaaa aatgccagag aactgacgct ggcgaacgcg gcaattactg acatacagat
     1861 gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag ctgatgcgaa
     1921 agctgaaaat gatgctctgc gtgatgatgt tgccgctggt cgtcgtcggt tgcacatcaa
     1981 agcagtctgt cagtcagtgc gtgaagccac caccgcctcc ggcgtggata atgcagcctc
     2041 cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagaga ggctgatcac
     2101 tatgcaaaaa caactggaag gaacccagaa gtatattaat gagcagtgca gatagagctg
     2161 cccatatcga tgggcaactc atgcaattat tgtgagcaat acacacgcgc ttccagcgga
     2221 gtataaatgc ctaaagtaat aaaaccgagc aatccattta cgaatgtttg ctgggtttct
     2281 gttttaacaa cattttctgc accaccacaa atttttgctg catcgacagt tttcttctgc
     2341 ccaattcccg aaacgaagaa atgatgggtg atggtttcct ttggtgttac tgctgtaggt
     2401 ttgtttccaa cagtaaacgt ctgttgagca catcctgtaa taagcattgc cagagcggca
     2461 gaaaacaaca tttttttcat cttattatcc tgcattgtta aaaacggcag aatcctatgt
     2521 gacaacaatt aaacgatagt taaatggatt gatgaaaatt aaaactacac aggtgggctc
     2581 agactattgg aggaagttgg ggacactcag aatcctgtgg aatgaaataa accggtctat
     2641 ccgtccatta cccttttagc tgcgctgtat cgtcgccgta ttcccgcatt aaccatgacc
     2701 gtagcccgac ggggaattcc ttctgcgtga gtgtgcggga ataattaaaa acgatgcaca
     2761 ccgggttttt accgcgttaa tgattcgcgg gtttatcccg gtgcgatggt ggaagaaaca
     2821 ggaagctgta ttacagaaag tgctactact gtatcccgat gcgatgtatg taatgtgagt
     2881 cagataatgg cacaggatgt ggtgatgtgg cagtctggaa cacaggatat attgtcagaa
     2941 taagacccgt aggaataaaa atgaaaagac gccttttact actttttctg ttatctgtcc
     3001 tggcagtggg atgctcgcag caaaaagctg atgagccccg gcaattagtg acggtgtatc
     3061 cacgatatcc ggaatatgct gcagcaaatt atatcaaggg gctggttgag gttaagttcg
     3121 atattggtgc tgatgggact gtgacacgga tcgtttttct ccgctcagag cctcataatt
     3181 tgtttcgtga tgaagtggtg aaggccatgg cgaaatggcg atttgaaaag aatcgcccct
     3241 gtcagggagt gaagagacaa tttatcttta cgccgtcacg tccttgatgc ttccaggtag
     3301 agaggggctg gaagcaggag aaaaatgaaa gagccagcgg ttatattttt gtcatggctg
     3361 acgaggaatg atggaagaag gcgttgtatg ccacacaacg cctcactgtt catttcttct
     3421 ttttctctgg tggaacccga tgaataagag ttgcactggt ttccgatgag atggcgatat
     3481 actcgggcaa agtatgctgg cagttttcca actggtcaaa aatacctgct ctcgtctgtt
     3541 gcaatgcctg cagcatgcgg cggcaatgcg ccttgctttt actaaccatc tttccttcct
     3601 ctatcagtcg ctgcgtgaac tcatcatgga ataccaggta aatgcggatg ttatcggttt
     3661 tggctacgca gcatagtaca aaacggacag gtgcatcccg ggacggggga ggcgtcacat
     3721 gtccctgtga tggttgttcc gggtaatgta ctgtgtgggg cataaaaatg tccgataatt
     3781 ttactttcta ccgcagttag ttgattcgtt ggtcctggta gcacattggg cgaggattta
     3841 aatgccaggc aactgaagga tgatgtcgca agggagatag cgagaatatt tctgattttc
     3901 atttgatgat gcctctgtgt gaaatgacgg taaacgacgc acttgtgccg gcacataata
     3961 gcaagcacca taatagatca gattcgattc ttgctgtaag tgataattat tctcgttttc
     4021 gggtcctttc cgtcgatcca acaggttacg gggcggcgac ctcgcgggtt ttcgctattt
     4081 atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg ccgtaaccta atgtttttat
     4141 ttaaaacacc ccctgaaaag aaaggaaacg acaggtgctg aaaacgggct ttttagcctc
     4201 tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag
     4261 ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga gcagggaatg
     4321 cccgttctgc gaggtggtgg gaagggtaat gaggtgcttt atgactctgc cgccgtcata
     4381 aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
     4441 gaactgcggc aggccagcga ggcagatctc cagccaggga ctattgagta cgaacgccat
     4501 cgacttacgc gtgcgcaggc cgacgcacag gaactgaaga atgccagaga ctccgctgaa
     4561 gtggtggaaa ccgcattctg tactttcgtg ttgtcgcgga tcgcaggtga aattgccagt
     4621 attctcgacg ggatccccct gtcggtgcag cggcgttttc cggaactgga aaaccgacat
     4681 gttgatttcc tgaaacggga tatcatcaaa gccatgaaca aagcagccgc gctggatgaa
     4741 ctgataccgg ggttgctgag tgaatatatc gaacagtcag gttaacaggc tgcggcattt
     4801 tgtccgcgcc gggcttcgct cactgttcag gccggagcca cagaccgccg ttgaatgggc
     4861 ggatgccaat tactatctcc cgaaagaatc cgcataccag gaagggcgct gggaaacact
     4921 gccctttcag cgggccatca tgaatgcgat gggcagcgac tacatccgcg aggtgaatgt
     4981 ggtgaagtct gcccgtgttg gttattccaa aatgctgttg ggtgtttatg cctacttcat
     5041 agagcataag cagcgcaaca cacttatctg gttgccgacg gatggtgatg ccgagaactt
     5101 tatgaaaacc cacgtcgagc cgaccatccg tgatattcca tcgctgctgg cgctggctcc
     5161 gtggtatggc aaaaagcacc gggataacac gctcaccatg aagcgtttca ccaatggtcg
     5221 tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt
     5281 ggcgggttat gatgaacttg ctgcctttga tgatgatatt gaacaggaag gctccccgac
     5341 gttcctgggc gacaagcgta ttgaaggctc ggtctggcca aagtccatcc gtggctcgac
     5401 gcctaaagtg agaggcacct gccagattga gcgtgctgcc agtgaatccc cgcattttat
     5461 gcgttttcat gttgcctgcc cgcactgcgg ggaggagcag tatcttaaat ttggcgataa
     5521 agagacgccg tttggcctca aatggacgcc ggatgatccc tccagcgtgt tttatctctg
     5581 cgagcataat gcctgcgtca tccgccagca ggatctggac tttactgatg cccgttatat
     5641 ctgcgaaaag accgggatct ggacccgtga tggcattctc tggttttcgt catccggtga
     5701 agagattgag ccgcctgaca gtgtgacctt tcacatctgg acagcgtaca gcccgttcac
     5761 cacctgggtg cagattgtca aagactggat gaaaacgaaa ggggatacgg gaaaacgtaa
     5821 aaccttcgtg aacaccacgc tcggtgagac gtgggaggcg aaaatcggcg aacgtccgga
     5881 tgctgaagtg atggcagaac ggaaagagca ttattcagcg cccgttcctg accgtgtggc
     5941 ttacctgacc gccggtatcg actcccagct ggatcgctac gaaatgcgcg tatggggatg
     6001 ggggccgggt gaggaaagct ggctgattga ccggcagatt attatgggcc gccacgacga
     6061 cgagcagacg ctgctgcgtg tggatgaggc catcaataaa acctacaccc gccggaatgg
     6121 tgcagaaatg tcggtatccc gtatctgctg ggatactggc gggattgacc cgaccattgt
     6181 gtatgaacgc tcgaaaaaac atgggctgtt cagggtgatc cccattaaag gtgcatccgt
     6241 atacggaaag ccggtggcca gcatgccacg taagcgaaac aaaaacgggg tttaccttac
     6301 cgaaatcggt acggataccg cgaaagagca aatttataac cgcttcacac tgacgccgga
     6361 aggggatgaa ccgcttcccg gtgccgttca cttcccgaat aacccggata tttttgatct
     6421 gaccgaagcg cagcagctga ctgctgaaga gcaggtcgaa aaatgggtgg atggcaggaa
     6481 aaaaatactg tgggacagca aaaagcgacg caatgaggca ctcgactgct tcgtttatgc
     6541 gctggcggcg ctgcgcatca gtatttcccg ctggcagctg gatctcagtg cgctgctggc
     6601 gagcctgcag gaagaggatg gtgcagcaac caacaagaaa acactggcag attacgcccg
     6661 tgccttatcc ggagaggatg aatgacgcga caggaagaac ttgccgctgc ccgtgcggca
     6721 ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac agaaagacgg acggagagtg
     6781 gagtttacgg ccacttccgt gtctgacctg aaaaaataca ttgcggagct ggaagtgcag
     6841 accggcatga cacagcgacg caggggacct gcaggatttt atgtatgaaa acgtccacca
     6901 ttcccaccct tctggggccg gacggcatga catcactgcg tgaatatgcc ggttatcacg
     6961 gcggtggcag cggatttggt gggcagttgc gggcgtggaa tccaccgagt gaaagtgtgg
     7021 atgcagccct gctgcccaac tttacccgtg gcaatgcccg cgcggacgat ctggtacgca
     7081 ataacggcta tgctgccaac gccatccagc tgcatcagga tcatatcgtc gggtcttttt
     7141 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     7201 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     7261 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     7321 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     7381 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     7441 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     7501 tcagcgagga cggctatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     7561 tacccggcgg gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagaccc
     7621 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     7681 acacgcagct gcagagcgcc attgtgaagg cgatgtatgc cgccaccatt gagagtgagc
     7741 tggatacgca gtcagcgatg gattttattc tgggcgcgaa cagtcaggag cagcgggaaa
     7801 ggctgaccgg ctggattggt gaaattgccg cgtattacgc cgcagcaccg gtccgtctgg
     7861 gaggcgcaaa agtgccgcac ctgatgccgg gtgactcact gaacctgcag acggctcagg
     7921 acacggataa cggctactcc gtgtttgagc agtcactgct gcggtatatc gctgccggac
     7981 tgggtgtctc gtatgagcag ctttcccgga attacgccca gatgagctac tccacggcac
     8041 gggccagcgc gaacgagtcg tgggcgcact ttatggggcg gcgaaaattc gtcgcatccc
     8101 gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc catcgcccgc cgcgtggtga
     8161 cgtcaccttc aaaagcgcgc ttcagttttc aggaagcccg cagtgcctgg gggaactgcg
     8221 actggatagg ctccggtcgt atggccatcg atggtctgaa agaagttcag gaagcggtga
     8281 tgctgataga agccgggctg agtacctacg agaaagagtg tgcaaaacgc ggcgatgact
     8341 atcaggaaat ttttgcccag caggtccgtg aaacgatgga gcgtcgtgca gccggtctta
     8401 aaccacccgc ctgggcggct gcggcatttg aatccgggct gcgacaatca acagaggagg
     8461 agaagagtga cagcagagct gcgtaatctc ccgcatattg ccagcatggc ctttaatgag
     8521 ccgctgatgc ttgaacccgc ctatgcgcgg gttttctttt gtgcgcttgc aggccagctt
     8581 gggatcagcc gcctgacgga tgcggtgtcc ggcgacagcc tgactgccca ggaggcactc
     8641 gcgacgctgg cattatccgg tgatgatgac ggaccacgac aggcccggag ttatcaggtc
     8701 atgaacggca tcgccgtgct gccggtgtcc ggcacgctgg tcagccggac gcgggcgctg
     8761 cagccgtact cggggatgac cggttacaac ggcattatcg cccgtctgca acaggctgcc
     8821 agcgacccga tggtggacgg cattctgctc gatatggaca cgccaggcgg aatggtggcg
     8881 ggggcatttg actgcgctga catcatcgcc cgtgtgcgtg acataaaacc ggtatgggcg
     8941 ctggccaacg acatgaactg cagtgcaggt cagctgcttg ccagtgccgc ctcccggcgt
     9001 ctggtcacgc agaccgcccg gacaggctcc atcggcgtca tgatggctca cagtaattac
     9061 ggtgctgtcc tggagaaaca gggtgtggaa atcacgctga tttacagcgg cagccataag
     9121 gtggatggca atccctacag ccatcttccg gatgacgtcc gggagacact gcagtcccgg
     9181 atggacgcaa cccgccagat gtttgcgcag aaggtgtcgg catataccgg cctgtctgtg
     9241 caggctgtgc tggataccga ggctgcagtg tacagcggtc aggaggccat tgatgccgga
     9301 ctggctgatg aacttgttaa cagcagcgat gcgatcaccg tcatgcgtga tgcactggat
     9361 gcacgtaaat cccgtctctc aggagggcga atgaccaaag agactcaatc aacaactgtt
     9421 tcagccactg cttcgcaggc tgacatcacc accgtggagc ctgcgaagga gggcgaaaac
     9481 gccagcgcgg cgcagccgga tgtgaacgca cagatcaccg cagcggttgc ggcagaaaac
     9541 agccgcatta tggggatcct caactgtgag gaggctcaca gacgcgaaga acaggcgcgc
     9601 gttctggcag aaacccccgg aatgaccgtg gaaacggccc gccgcattct ggccgcagca
     9661 ccacagagtg cacaggcgcg cagtgacact gcgctggatc gtctgatgca gggtgcaccg
     9721 gcaccgctgg ctgcaggtaa cctggcatct gataccaata aagaattact taatacacct
     9781 gaagctttac cggtataaga ggcagttatg gcgacaaaag aagagtttaa ccattaccag
     9841 ccgctgggta acagtgatcc ggctcataca gcaattgcgc ctggcggatt gagtgcgaaa
     9901 acgcctgcaa tgaccccact gatgctggat ggcactaccc gtaagctggt tgtgtgggat
     9961 ggcaccaccg acggtgcagc cgttggcatt cttgcggttg ctgctgacca gaccagcacc
    10021 acactgacgt tctacaagtc cggctcgttc cgttatgagg atgtgctctg gccggaggct
    10081 gccagcgacg agacgaaaaa acggaccgcg tttgccggaa cggcaatcag catcgtttaa
    10141 tcttcccctt catcaacaaa ggccgcctgt gcggcttttt ttatggaaat aatttatgtc
    10201 tgtatataca actgcagaat tactggcatc gacccagcat cactttaagt tcgatccgct
    10261 gtttctgcgc ctgtttttcc gtgaaaccta tcctttcacc acggaaaaag tctatctctc
    10321 acaaattccg ggactggtaa acatggcgct gtacgtttcg ccgattgttt ccggtgaggt
    10381 tatccgatcc cgtggcggct ccacctctga atttacgccg ggttatgtca aacccaagca
    10441 tgaggtgaat ccgcagatga ccctgcgtcg cctgccggat gaagatccgc agaacctggc
    10501 tgacccggct taccgtcgtc gccgtattat tcggcagaat atgctggatg aaaatctggc
    10561 gattgcccag gtcgaagaga tgcaggcagt ttctgccgtg cttaagggca aatataccat
    10621 gaccggtgaa gccttcgatc cggttgaggt ggatatgggc cgcagtgagg cgaataacat
    10681 cacgcagtcc ggcggcacgg agtggagcaa gcgtgacaag tccacgtatg acccgaccga
    10741 cgatatcgaa gcctacgcgc tgaacgccag cggtgtggtg aatatcatcg tgtttgatcc
    10801 gaaaggctgg gcgctgttcc gttccttcaa agccgtcaag gagaagctgg atacccgtcg
    10861 cggctctcat tccgagctgg agacagcggt aaaagacctg ggcaaagcgg tgtcttataa
    10921 gggaatgtat ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt
    10981 caaaaagaac ttcctgccgg acaacacgat ggtgctgggg aacactcagg cacgcggtct
    11041 gcgcacctat ggttgcattc aggatgcgga cgcacagcgc gaaggcatta acgcctctgc
    11101 ccgttacccg aaaaactggg tgaccaccgg cgacccggcg cgtgagttca ccatgattca
    11161 gtcagcaccg ctgatgctgc tggctgaccc tgatgagttc gtgtctgtac aactggcgta
    11221 atcatggccc ttcggggcca ttgtttctct gtggaggagt ccatgacgaa agatgaactg
    11281 attgcccgtc tccgctcgct gggtgaacaa ctgaaccgtg atatcagcct gacggggacg
    11341 aaagaagaac tggcgctccg tgtggcagaa ctggaagaag agcttgatga cacgggcgac
    11401 actgccggtc aggatatccc tctcagcccg gaaaatgtgc tgaccggaca tgaaaatgag
    11461 gttgtatcag cgcagccgga taccgtgact gatacggctg atctggtcac ggttgtggca
    11521 ctggtgacgc tgcatactga tgcacttcac gccacgcggg atgaggctgt ggcatttgtg
    11581 ctgccgggaa cggcgttccg tgtctctgcc ggtgtggcag ctgaaatgac agagcgcggc
    11641 ctggccagaa tacaataacg ggaggcgctg tggctgattt cgataacctg ttcgatgctg
    11701 ccattgcctg cgccgatgaa acgatacgcg ggtacatggg aacgtcagcc accatgacat
    11761 ccggtgagca gtccggtgct gtgatacgtg gtgtttttga tgaccctgaa aatatcagct
    11821 atgccggaca gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg
    11881 atgtgcggca gctgcggcgc ggagacacgc tgaccatcgg tgaggaaaac ttctggatag
    11941 accgggtttc gccggatgat ggcggaagtt gttatctctg gcttgggcgt ggcgcgccgc
    12001 ctgccgttaa ccgtcgtcgc tgaaagggga tgtatggtca taagaggtct tgagcaggcc
    12061 gttgggaacc tcagccgtat cagcaaaaaa gcagtgcccg gcgccgccgc aatggccatt
    12121 aaccgcgttg cttcatccgc gatatcgcag tcggcgatac aggttgcccg tgagacaaag
    12181 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt caaaaacccg
    12241 ctggccagaa tcagggttaa ccggggggat ttgcccgtaa tcaggctggg taacgcgcgg
    12301 gttgtcctgt cccgacgcag gcgtcgtaaa aaggggcagc attcagccct gaaaggtggc
    12361 ggcagcgtgc ttgtggtggg aaaccgtcgt attcccggcg cgtttattca gcaactgaaa
    12421 aatggcaggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
    12481 gtgaaaatcc cgatggcggt gccgctgacc acggcgttta aacagaatat tgagcggata
    12541 cggcgtgagc gtcttccgaa agagctgggc tatgcgctgc agcatcaact gaggatggta
    12601 ataaagcgat gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg
    12661 acaccggggc gacgtttttt gatggtcgcc ccgctgtttt tgatgaggcg gattttccgg
    12721 caattgccgt ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct
    12781 ggcaggcgga gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg
    12841 atgcgtggat ggagtcccgg atttatccgg tgatgagcga tatcccggca ctgtcagatt
    12901 tgatcaccag tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt
    12961 ggagttcagc cgatctgact tatgtcatta cctatgaaat gtgaggacga tatgcctgta
    13021 ccaaatccta caatgccggt gaaaggtgcc gggaccacac tgtgggttta taaggggaac
    13081 ggtgaccctt atgcgaaccc gctttcagac gtggactggt cgcgtctggc aaaagttaaa
    13141 gacctgacgc ccggcgaact gaccgctgag tcctatgacg acagctatct cgatgatgaa
    13201 gatgcggact ggactgcgac cgggcagggg cagaaatcag ccggagatac cagcttcacg
    13261 ctggcgtgga tgcccggaga acaggggcag caggcgctgc tggcgtggtt taatgaaggt
    13321 gatacccgtg cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgtggctgg
    13381 gtcagcagta tcggtaaagc ggtgacggcg aaggaagtga tcacccgcac ggtgaaggtc
    13441 accaatgtgg gacgcccgtc gatggcagaa gatcgcagca cggtcacagc ggcaaccggc
    13501 atgaccgtga cgcctgccag cacctcggtg gtgaaagggc agagcacgac gctgaccgtg
    13561 gcattccagc cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca
    13621 aaagccaccg tgtcggtcag tggtatgacc atcaccgtga acggcgttgc tgcaggcagg
    13681 gtcaacattc cggttgtatc cggtaatggt gagtttgctg cggttgcaga aatcaccgtc
    13741 accgccagtt aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt tgaacataac
    13801 ggcgtgaccg tcacgctttc tgaactgtca gccctgcagc gtattgagca tcttgccctg
    13861 atgaaacggc aggcagaaca ggcggagtca gacagcaacc ggcaggttac tgtggaagac
    13921 gtcatcagaa ccggtgcttt tgtggtggcg atgtccctgt ggcataacca tccgaagaag
    13981 acgcagatgc cgtccatgaa tgaggccgtt aaacagattg agcaggaagt gcttaccacc
    14041 tggcccacag aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggtatgtct
    14101 gagtttgtgg tgaataatgc ccctgaacag gcagaggacg ccgggcctgc agagcctgtt
    14161 tctgcgggaa agtgttcgac ggtgagctga gttttgccct gaaactggcg cgtgagatgg
    14221 ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc
    14281 accgctttta cagtacccac tattttcatg atgttctgct ggatatgcac ttttccgggc
    14341 tgacgtacac cgtactcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca
    14401 gtctgctgaa ccggcgtgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag
    14461 cggcagggct tgccggaggc gtccgctttg acccggacgg gaatgaagtt atccccgctt
    14521 ccccggatgt gacgggcatg acggaggatg acgtaatgct gatgacagta tcagaaggga
    14581 tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg
    14641 gatgcggcca gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa
    14701 actgatgcga aaaaaacagc ggcagtcgtt gaacagtcga tgaaccggca ggcgctggct
    14761 gcacagaaag cggggatttc cgtcgggcag tataaagctg ccatgcgtat gctgcctgcg
    14821 cagttcaccg acgtggccac gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg
    14881 caacagggtg gtcaggttaa ggactccttc ggcgggatga tccccatgtt ccgggggctt
    14941 gccggtgcga tcaccctgcc gatggtcggg gccacctcgc tggcggtggc gaccggtgcg
    15001 ctggcgtatg cctggtatca gggtaactca accctgtccg atttcaacaa aacgctggtc
    15061 ctttccggta atcagtcggg tctgacggca gatcgtatgc tggtcctgtc cagagccggg
    15121 caggcggcag ggctgacgtt taaccagacc agcgagtcac tcagtgcact ggttaaggcg
    15181 ggggtaagcg gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct
    15241 gcatccggcg tggaggtgga caaggtcgct gaagccttcg ggaagctgac cactgacccg
    15301 acgtcggggc tgacggcgat ggcgcgccag ttccataacg tgacggcgga gcaaattgcg
    15361 tatgttgctc agttgcagcg ttccggcgat gaggccgggg cattgcaggc agcgaacgag
    15421 gccgcaacga aagggtttga tgaccagacc cgccgcctga aagagaacat gggcacgctg
    15481 gagacctggg cagacaggac tgcgcgggca ttcaaatcca tgtgggattc ggtgctggat
    15541 attggtcgcc cggacacagc ccaggaaatg ctggagaaag cagaaaaggc ttttgatgag
    15601 gcggacaaaa aatggcagtg gtatcagagc cggagccacc ggcgcggtaa aacatccgca
    15661 tttcttgcta atctccgggg agcatgggag gacagagcga atgcgcaact tgggctttca
    15721 gccgccacgt tgcaggccga tcttgaaaag gccagagaga tggcagcaaa ggactgggcc
    15781 gagtctgagg catcacggct gaaatatacc gaagaggcgc agaaggctta tgaacgcctg
    15841 cagacgccgc tggagaaata taccgcccgt caggaagaac tgaataaggc cctgaaagac
    15901 ggaaaaatcc tgcagacaga ttacaacacg ctgatggcgg cggcgaaaaa ggactatgaa
    15961 gcgacactga aaaagccgaa gcagtccggc gtgaaggtgt ctgcaggaga gcgtcaggaa
    16021 gacagtgctc atgccgccct gctgacgctt caggcagaac tccggacgct ggagaagcat
    16081 gccggagcga atgagaaaat cagccagcag cgccgggatt tgtggaaggc ggagagtcag
    16141 ttcgcggtac tggaggaagc ggcgcaacgt cgccagctgt ctgcacagga gaaatccctg
    16201 ctggcgcata aagatgagac gctggagtac aaacgccagc tggctgcact tggcgacaag
    16261 gtcacctatc aggagcacct gaacgcgctg gcacagcagg cggataaatt cgcacagcag
    16321 caacgggcaa aacgggccgc cattgatgca aaaaaccggg ggctgactga ccggcaggca
    16381 gcgcgggaag ccacggaaca gcgcctgaag gaacagtatg gcgataattc tctggcgctg
    16441 aataacgtca tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc
    16501 tggatggcag gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg
    16561 tcgcaggtaa aaagtgctgc cacgcagacc tttgatggca ttgcacagaa tatggcggcg
    16621 atgctgaccg gcagtgagca gaactggcgc agcttcaccc gttccgtgct gtccatgatg
    16681 acagaaattc tgcttaagca ggcaatggtg gggattgtcg gaagtatcgg cagcgccatt
    16741 ggcggcggcg catcagcgtc aggcgttaca gccattcagg cagctgcggc gaaattccat
    16801 tttgcgaccg gaggatttac gggaaccggc ggcaaatatg agccagcggg gattgttcac
    16861 cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtagg aaatctttac
    16921 cggctgatgc gcggctatgc caccggtggt tatgtcggtg gcaccggagg tccggcgcaa
    16981 atgcggcgtt cagagggtat cagatttgag cagaacaaca acgtggtgat tcagaacgac
    17041 ggtacgaatg gtctgccagg tccacagatg ctgaaggcag tgtatgacat ggcccgcaag
    17101 ggtgcccgtg atgaaattca ggcacagatg cgcgatggtg gtctgttctc cggaggtgga
    17161 cgatgaagac cttccgctgg aaagtgaaac ccggtatgga tgtggcttca gccccttccg
    17221 tcaggaaagt gcgctttggt gatggctatt cccagcgagc gcctgccggg ctgaatgccg
    17281 acctgaaaac gtacagcgtg acgctttctg ttccccgttg ggaggccacg gcgctggagt
    17341 cgtttctggc tgagcacggg ggctggaaag cctttctgtg gacgccgcct tatgagtggc
    17401 ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg cgtgttgagt
    17461 tcagcgcaga gtttgaacag gtggtgatct gatgcaggat atccggcagg aaacactgaa
    17521 tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg atctgacaga
    17581 ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
    17641 ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg aactgaatgg
    17701 caaaggcacc agtacgcgcc ccacgctgac ggtttctaac ctgtacggta tggtcaccgg
    17761 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtcaggcgta aggtttacgc
    17821 ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc cggagcagga
    17881 ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga gtgcctcttt
    17941 tgtactgtcc acgccgacgg aaacggatgg tgctgttttt ccggggcgca tcatgctggc
    18001 cagcacctgc acctggacct atcgcggtga tgagtgcggt tatagcggtc cggcggtcgc
    18061 ggatgaatat gaccagccga cgtccgatat cacgaaggat aaatgcagca aatgcctgag
    18121 tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact
    18181 ttcgcagtaa atcccatgac acagacagaa tcagcgattc tggcgcacgc ccggcgatgt
    18241 gcgccagcgg agtcgtgcgg cttcgtggta agcacgccgg agggggaaag atatttcccc
    18301 tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga agactggctg
    18361 caggcagaaa tgcagggtga gattgtggcg ctggtccaca gccaccccgg tggtctgccc
    18421 tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg gtggctggtc
    18481 tgccggggga cgattcataa gttccgctgt gtgccgcatc tcaccgggcg gcgctttgag
    18541 cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc ggggattgag
    18601 atgccggatt ttcatcgcga ggatgactgg tggcgtcacg gtcagaatct ctatctggat
    18661 aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc gggcgatgtg
    18721 ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg tggtgatggc
    18781 gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac cgacaaatgg
    18841 cagcgacgca cacactccct ctggcgtcac cgggcatggc gcgcatctgc ctttacgggg
    18901 atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct gaagccatcc
    18961 gcgcactggc cacacagctc ccggtgtttc gtcagaaact gagcgacggc tggtatcagg
    19021 tacggattgc cgggcgggac gtcagcacgt ccgggttaac ggcgcagtta catgagactc
    19081 tgcctgatgg cgctgtgatt catattgttc ccagagtcgc cggggccaag tcaggtggcg
    19141 tattccaggt tgtcctgggg gctgccgcca ttgccggatc attctttacc gctggagcca
    19201 cccttgcagc atggggggca gccattgggg ccggtggtat gaccggcatc ctgttttctc
    19261 tcggtgccag tatggtgctc ggtggtgtgg cgcagatgct ggcaccgaaa gccagaactc
    19321 cccgtacaca gacaacggat aacggcaaac agaacaccta tttctcctca ctggataaca
    19381 tggttgccca gggcaatgtt ctgccggttc tgtacggtga aatgcgcgtg gggtcacgcg
    19441 tggtttctca ggagatcagc acggcagacg aaggggatgg tggtcaggtt gtggtgattg
    19501 gtcgctgatg caaaatgttt tatgtgaaac cgcctccggg cggttttatc gtttatggag
    19561 catgacgaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc
    19621 tgaagtccac gcagctgctg agtgtgatcg atgccatcag cgaagggccg gttgaaggtc
    19681 cggtggatgg attaaaaagc gtgctgctga acagtacgcc ggtgctggac agtgagggga
    19741 ataccaatat ctccggtgtc acggtggtgt tccgggcagg tgagcaggag cagactccgc
    19801 cggaggggtt tgaatcctcc ggttccgaga cggtgctggg tacggaagtg aaatatgaca
    19861 cgccgatcac ccggaccatc acgtcggcaa acatcgaccg tctgcgcttt accttcggtg
    19921 tgcaggcact ggtggaaacc acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc
    19981 tggttcagat acaacgtaac ggtggctggg tgacggaaaa agacatcacc attaagggca
    20041 aaaccacctc gcagtatctg gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta
    20101 atatccggat gcgcaggatg acgccggaca gcaccacaga ccagctgcag aacaaaacgc
    20161 tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcactgg
    20221 tcggcgtgca ggtggactcg gaacagttcg gcagccagca ggtgagccgt aattatcatt
    20281 ttcgcgggcg catcctgcag gtgccgtcga actataaccc acagacgcgg caatacagcg
    20341 gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg tgtctgtggg
    20401 atatgctgac ccatccgcgc tatggcatgg ggaaacgttt tggtgcggca gatgtggata
    20461 aatgggcgct gtatgtcatc ggtcagtact gcgaccagtc ggtgcctgac ggatttggcg
    20521 gcacggagcc gcgcatcacc tgtaatgcgt acctgaccac acagcgcaag gcgtgggatg
    20581 tgctcagtga tttttgctcg gcgatgcgct gtatgccggt atggaacggg cagacgctga
    20641 cattcgtgca ggaccgaccg tcggataagg tgtggatcta taaccgcagt aatgtggtga
    20701 tgccggatga tggcgcgccg ttccgctaca gcttcagcgc cctgaaagac cgccataatg
    20761 ccgttgaggt gaactggatt gacccggata acggctggga gacggcgaca gagcttgtgg
    20821 aggacacgca ggccattgcc cgttacggtc gtaacgtcac gaagatggat gcctttggct
    20881 gtaccagtcg ggggcaggca caccgcgccg ggctgtggct gattaaaacg gagctgctgg
    20941 aaacgcagac cgtggacttc agcgtgggcg cagaagggct tcgtcatgta ccgggcgatg
    21001 tcattgaaat ctgcgatgat gactatgcgg gtatcagcat cggcgggcgc gtgctggcgg
    21061 tgaacagcca ggcgcggaca ctgacgctcg accgtgaaat catgctgcca tcctccgcca
    21121 ccacgctgat aagcctggtt gacggaaatg gcaatccggt cagcgtggag gtccagtccg
    21181 tcaccgacgg cgtgaaggtg aaagtgagcc gtgttcctga cggcgttgcc gaatacagcg
    21241 tgtgggggct gaagctgccg acgctgcgcc agcgcctgtt ccgctgtgtg agtatccggg
    21301 agaacgatga cggtacgtat gccatcaccg ccgtgcagca tgtaccggaa aaagaagcca
    21361 tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat ggtgtcacgc
    21421 cgccagcggt gcagcacctg accgccgaag tcaccgcaga cagcggggaa tatcaggtgc
    21481 tggcgcgatg ggacacgccg aaggtggtga agggcgtgag cttcctgctt cgccttaccg
    21541 tggcagcgga cgacggcagt gagcggctgg tcagcatggc ccggacgacg gaaaccacat
    21601 accgcttcag gcaactggcg ctggggaact acaggctgac agtccgggcg gtaaatgcgt
    21661 gggggcagca gggcgatccg gcatcggtat cgttccggat tgccgcaccg gcagcgccgt
    21721 cgcagattga gctgacaccg gggtattttc agataaccgc cacgccgcat cttgcggttt
    21781 atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg gatatcaggc
    21841 aggttgaaac cacagcacgc tatcttggca cggcgctgta ctggatagcc gccagtatca
    21901 atatcaaacc gggccatgat tattattttt acatccgcag tgtgaacacc gttggcaaat
    21961 cggcatttgt ggaggctgtt ggccagccga gtgatgatgc atccggctat ctgaattttt
    22021 tcaaaggaga gatagggaaa acccatctgg ctcaggagtt gtggacgcag attgataacg
    22081 gtcagcttgc gcctgacctg gctgaaatca ggacgtccat tacgggtgtc agcaatgaaa
    22141 tcacgcagac cgtcaataag aaactggaag accagagtgc agcgatccag cagatacaga
    22201 aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcagcaga
    22261 tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaat acccctgacg
    22321 gtatgcagag tcaggtgctg ctggcggcgg acaggattgc gatgattaat cctgcgaatg
    22381 gcaatacaaa gccgatgttt gttggtcagg gcgatcagat atttatgaat gaagtgttcc
    22441 tgaaatatct gacggctccc accattacca gcggcggtaa tcctccggca ttttccctga
    22501 caccggacgg gcggctgacg gcgaaaaatg cggatatcag cggtaacgtg aacgcgaact
    22561 ccgggacgct caacaatgtc acgattaatc agaactgccg gattctggga aaactgtcag
    22621 ccaaccagat tgaaggcgat attgttaaaa cggtgggaaa agcctttccg agaaatagca
    22681 gttatgccag cggtacaata acggtcacgg tgtacgatga ccaggctttt gaccgtcaga
    22741 tagtaatccc ccccgttctg tttcgcggtg gtaagcatga aaacttcaac agcaacaacc
    22801 aacagtcata ctggtattca acctgtaagc tgcaggtgct gaagaacgga caggaaatct
    22861 ttcagcaacc cgcgacggat gtcagcaggg tattttcatc agtcattgat atgcctgccg
    22921 gacacggcca tgtcaccctg actttcaatg tttcttcata tggtgctaat aactggacgc
    22981 caacgaccag tatcagcgac cttcttgttg ttgtgatgaa gaaatctaca gccggtatca
    23041 gtatcagttg aattttataa cccagatacg ggtgccagaa atggcgcctt ttttatttgt
    23101 ggagtgaata tggcagtaca gatttcaggc gtgctgaaag acggtgcagg aaaaccgata
    23161 cagaactgca ccattcagct caaagcaaaa cgtaacagca ccacggttgt ggtgaacacg
    23221 gtggcctcag aaaatccgga tgaagccgga cgttacagca tggatgtcga gtatggccag
    23281 tacagcgtta tcctgctggt tgaaggtttt ccgccttcac atgccgggac catcaccgtg
    23341 tatgaggatt ctaagccggg gacgctgaat gattttctgg gcgctgcaac agaagatgat
    23401 gttcgtccgg aggcactgta tcgttttgaa aagatggtgg aagaggtggc acgcaacgct
    23461 gaagccgcct ctcagagcgc agcggcagca aagaaatcag aaacagcagc ggcatcgtcc
    23521 aggaatgcgg cgaaaacatc agagacgaat gcaggtaaca gcgcgaaagc ggcagcttct
    23581 tcaaaaacag ccgcacaaaa cgcagcaaca gcggcagaac gttcagagac aaatgcccgt
    23641 gcgtcagaag aagcctccgc agacagtgaa gaggcttccc gccgtaatgc agagtcagcc
    23701 gctgaaaatg ccggagtcgc caccacaaaa gcgcgggagg ccgcagcaga cgcaacaaag
    23761 gccgggcaga aaaaggatga ggctctgtcg gcagcgacac gggctgaaaa ggcggcagac
    23821 cgcgcagaag tcgcagcgga agtgactgca gagccctatg cgaatatagt gccgccgctg
    23881 cctgatgtgt ggataccgtt taacgattca ctggatatga ttgcgggttt ttctccgggc
    23941 tataaaaaaa tagctattgg tgacgatgtg gttcaggtcg ccagtgataa acaggttaat
    24001 ttcagtcgcg catcaacggc aacatatatc aacaaatctg gcgaactgaa aacggcggaa
    24061 attaatgagc cgcgatttga gtgtgatggc ctgcttattg agggacaaag aacgaactac
    24121 atgctcaatt cggaaagtcc agccagctgg gggaagtcat caaacatgga tgtgcccgaa
    24181 accgggacag atagttttgg ttttacttat ggaaagtttg tctgcaacga ttctctggtt
    24241 gggcaaactt cggctattaa tatggcatca attgctgcaa caaagtcagt tgatgtctca
    24301 ggcgataaca agtacgtgac aacctcatgc cgttttaaaa cagaacgaca ggtaaggtta
    24361 cgtatacggt ttgataagta tgatggtagt gcaacaactt ttcttggcga tgcgtacatt
    24421 gatacgcaaa cgcttgaaat taatatgaca ggtggggctg ccggcagaat tacggcacga
    24481 gtcaggaagg ataagaccac aggctggatt tttgcagagg caacgattca ggcaattgat
    24541 ggtgagttaa aaataggctc tcagatacag tattctcctg agcagggtgg ggcaacagta
    24601 tctggtgact atatttatct tgccacccca caagtagaga atgggccgtg tgtatcatca
    24661 tttattattt caggaggcag cgcaacgaca agagccagtg atttggttag tatccccacc
    24721 agaaataatc tttataagtt accatttact tttttacttg agattcataa aaactgggat
    24781 attgcaccaa acgccgcacc ccgcgtgtgg gatatagcag cagccaatac cgggcaatca
    24841 gcaattgcag caatcaacag aggtagtggt aagttatata tgagtctgtc aaacccttca
    24901 ggctcgtatg ttaatagcgc agcgacagat gtatttgcag agaaaaccac atttggatgt
    24961 attgcaaaag ctgatggtca ctttcatgtg gtgacaaatg gtaaagcggt taatgaagtt
    25021 tattgtgaat ataatggcgt gaccgctgat aaaaatatcc gatttggagg gcagacgaac
    25081 actggagaac gacatctgtt tggccatatt cgcaatttcc gcatatggca taaagaatta
    25141 aatgacaggc aattaaaaga ggtcgtatga aagatttaac tttgaagttt catgacaaac
    25201 tgcagtttaa ggccttcctg tcatctcttg gctgggcgga agatgaagac ctccagaata
    25261 aactgttagt tgatgaaatt ggtttcacct acacagaaac aggggtaaca gaagagggag
    25321 aacctgtctg tatccggaat gatggttatt ttgtcaacat tcgcattctt gatgacttgt
    25381 ttgatgtttc tgtattctct gattatgtcg tggagctgga aacaccgctt cgggaatgga
    25441 gctgaaagga ggaaataatg gatataagcc ccttacttca tgcactttgt gctgtggctg
    25501 cgcagatact ggttggtctt tttaccggaa actgggctta cggagcgata gccggttgta
    25561 cgttcttcat tgcgcgtgaa catacccagg cagaatatcg ctggattgaa atgttcgggc
    25621 atggcaagcg aatgaatatg ccgtggtggg gcggttttga tccgcgtgct tgggatgtgg
    25681 caagcctgat ggattttgct gtgccggtgg tggcgtgtct gctggtctgg ctgttgatta
    25741 atcgtgggtg aaaaaaggtg agctgtatat gcaacggagg aagaaacccc gttgctggaa
    25801 gccaggaaaa agtatcgggt gttgctgtcc tcggatgttg tgctaattgg gtgataacag
    25861 atcctgtgta tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg caaacgtcat
    25921 gatgaacaaa accgcgaata agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa
    25981 ggacaaccag acaccagtca ttgacactat taccgatatg ccagtcgcca gtaggagcgc
    26041 aatatgaaag cgttgtgtca ggagtaccgc aatggcgcca ggcgcaatga gcagagaaat
    26101 ggagagaatg atacctaccg ctttcagcgt cgccacaatg gtcagagaga ccatgcacag
    26161 cagcccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa
    26221 gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca gcccggcaat
    26281 aatcatcgtc tggattatat cgccgatggt tatcccgagc atgtcgccga aaagaatatg
    26341 gtcaagatgt acgtctggtt tgactgcaat atacaagatc agacctgcgg caaacatgcc
    26401 agaaaaaaca atccccatca ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc
    26461 cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccaaag gtaaccccag
    26521 aatccaggca agcacgatgc caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc
    26581 ccagcctttc aggaccagaa agaccgacag cagcgcacag ggtatggaaa ctaccagtga
    26641 aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata atgccatcat
    26701 gagcgatcct ccagcgcctt atgcgcacgc cgacgatttg ccaaaagccc atgggtgggc
    26761 gcaaagacaa atgctaaaag aaacagcagt gcctgcgcca ccacgatgat tccacctgtc
    26821 gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tagttacact gccaatagtt
    26881 acagcaatca tgagcaggcg gggaaaacga tcggttaaca accatgctgt tgcaccggga
    26941 gtcaccacca gacagatcac cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa
    27001 accgacagca gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc aatagcacgg
    27061 gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa aagcacgatg
    27121 atcgagagaa tgccaatgat agtgagttgc aggatatccg ccggatcgat tgccagaata
    27181 ttgcccagga cgatggtctg aatgttcaca gatgtcgggt tcaatgacac cataaacagc
    27241 cccaggccaa aaaaggacga gaagatcaga ccgatgatgg catcttcttt taagcgggtg
    27301 cgttggttaa gaaacagcat gctgcccgca gccagtccgc cggaaaagaa ggctcccaga
    27361 gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga gtgcgacaac
    27421 gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa agcgcagaga
    27481 ccgccgacca tcgccgagac ccacatcgca ttgagcatat attcatagct gaagggttcc
    27541 agtagcacgt tcatctttct tccctctgca ccgctgacgg tcgatgagcc acaaaagggc
    27601 gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg cgcagtacgc
    27661 cgctaaaagc cagctctagg ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca
    27721 ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgacggat ccgagattgt
    27781 gtgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg ctgatgattt
    27841 tggcttcggt tttcacatca acgccagtaa aaggttcatc cagcaggatc acatcaccct
    27901 gctgtgcaat cgctctcgcc agaaagacgc gtttcttctg tccaccggaa agctcgccga
    27961 tttgtcgatg gcgaaaattc accatatcga cacgttccag cgcatcggtg acaatttgac
    28021 gatccctctt tttggcgata cgcaacatac ccatatgccc atagcggccc atcatcacca
    28081 catcttccac cagtacagga aatgaccagt caacctcttc tgactgaggg acgtaggcaa
    28141 ccaggttttt ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc tttccgctgg
    28201 ttagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa ccgtttaccc
    28261 caaccagagc ggcaatagat ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt
    28321 gcccgttacg ccaggtgaca gtaacatcat taacgacaat gcctgcagat tgcatcatta
    28381 tttctccctt ttcccggctt taattccctg taccagcgta ctggtagtaa ccttaagaag
    28441 gtcgatatat gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat agagcacacc
    28501 gccgtagtgt gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact
    28561 ctcgctaaag actgccggga tatgattttt cttaactata tcaacaacct tacgtacctg
    28621 ctgcggtgtt ccttgttgat cggcattaat cggccacaga taaagctctt ttagccccaa
    28681 atcgcgtgcg agataagaaa aagccccttc actggtgacc atccaccgct gattctcagg
    28741 gagttccgta atctgcttac gcaggggggc aagggtttgg gtaatcttgg ctttataagt
    28801 atcggcatta cgttgatagg tttgtgcatt agccggatcg tattttatca acgcatcacg
    28861 aatattatcg acgtaaatca gagcattatc tggcgacatc catgcatggg ggttaggttt
    28921 gccctcatag gggccttcgg tgatccctac tggcgtcaca cccgaagaga caattacttc
    28981 tggaacccca ttgagatgct ggtaaaagcg ttggaaccac aattccagat tcataccatt
    29041 ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg ccaggggtag gctgatactc
    29101 atgaatttct gcaccaggct tggttatgga tgagacctct gcagcatctc cagccacgtt
    29161 tttggccata tccgcgatga tggtaaatgt tgtaataacc ttgaattttt cagttgcact
    29221 tgcctgaaat gcgatcgagc aggtgagggc gaggcccccc aagagcatgg ttactttttt
    29281 tattgagtgc ataatttagt acctatagtg agcaaaatta gaacaaacat agcaacggct
    29341 atgttttata gcataaccta tacaaaatga aaatgattat cattaacaac tttgggccca
    29401 aaacagggat tagtttgttt cctgctggat gaaaggactc ttgggatagc acatgccgca
    29461 cgaagagcag cattacatga tgttttgtcg aatgagctac gaatcctgat agaaatacga
    29521 aacaagctag cacatggaca atggatctat cccttcaata atgaaggaaa tactgtggac
    29581 cctgataaat ttaaattaat cgataaagaa aacttgcagt cacttcaatt caaatattca
    29641 ttagcacagc atttagctga tgctgtacat gatttggttg taagtcctac gacattcgaa
    29701 cgagatttcg aaagccattt taaaaattct tttacgtccg ctcctcgctc ataacaggca
    29761 ttcactacat ttgtcttcat tggctatgca agtcgtaata caaggtgggg caaaactgag
    29821 acacataagg tctctcacaa aggtctgcaa ggcctcacat gtattgagat gttaataaaa
    29881 tgtagacttg taattttgat ataaatggta gataaaacct ttccccaaaa taaaaacgaa
    29941 cgtcaatgaa at
//
LOCUS       DAJOTB010000047        29850 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJOTB010000047 DAJOTB010000000
VERSION     DAJOTB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.047"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(43..828)
                     /locus_tag="OHN28_002059"
     CDS             complement(43..828)
                     /locus_tag="OHN28_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2297458.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1147..2424
                     /locus_tag="OHN28_002060"
     CDS             1147..2424
                     /locus_tag="OHN28_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2297459.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            2451..3929
                     /locus_tag="OHN28_002061"
     CDS             2451..3929
                     /locus_tag="OHN28_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2297460.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            4094..5014
                     /locus_tag="OHN28_002062"
     CDS             4094..5014
                     /locus_tag="OHN28_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCQ2297461.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            5182..6699
                     /locus_tag="OHN28_002063"
     CDS             5182..6699
                     /locus_tag="OHN28_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2297462.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            6760..8130
                     /locus_tag="OHN28_002064"
     CDS             6760..8130
                     /locus_tag="OHN28_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCQ2297463.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            8130..9539
                     /locus_tag="OHN28_002065"
     CDS             8130..9539
                     /locus_tag="OHN28_002065"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCQ2297464.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            9565..10572
                     /locus_tag="OHN28_002066"
     CDS             9565..10572
                     /locus_tag="OHN28_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2297465.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(10657..11328)
                     /gene="queE"
                     /locus_tag="OHN28_002067"
     CDS             complement(10657..11328)
                     /gene="queE"
                     /locus_tag="OHN28_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCQ2297466.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            11467..11608
                     /locus_tag="OHN28_002068"
                     /pseudo
     CDS             11467..11608
                     /locus_tag="OHN28_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11622..12494
                     /locus_tag="OHN28_002069"
     CDS             11622..12494
                     /locus_tag="OHN28_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCQ2297467.1"
                     /translation="MRYFILIFTLVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(12554..13852)
                     /gene="eno"
                     /locus_tag="OHN28_002070"
     CDS             complement(12554..13852)
                     /gene="eno"
                     /locus_tag="OHN28_002070"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCQ2297468.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(13940..15577)
                     /gene="pyrG"
                     /locus_tag="OHN28_002071"
     CDS             complement(13940..15577)
                     /gene="pyrG"
                     /locus_tag="OHN28_002071"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCQ2297469.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(15805..16596)
                     /gene="mazG"
                     /locus_tag="OHN28_002072"
     CDS             complement(15805..16596)
                     /gene="mazG"
                     /locus_tag="OHN28_002072"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCQ2297470.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(16692..18926)
                     /locus_tag="OHN28_002073"
     CDS             complement(16692..18926)
                     /locus_tag="OHN28_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCQ2297471.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(18974..20275)
                     /gene="rlmD"
                     /locus_tag="OHN28_002074"
     CDS             complement(18974..20275)
                     /gene="rlmD"
                     /locus_tag="OHN28_002074"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCQ2297472.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            20332..23088
                     /gene="barA"
                     /locus_tag="OHN28_002075"
     CDS             20332..23088
                     /gene="barA"
                     /locus_tag="OHN28_002075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCQ2297473.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(23319..24659)
                     /gene="gudD"
                     /locus_tag="OHN28_002076"
     CDS             complement(23319..24659)
                     /gene="gudD"
                     /locus_tag="OHN28_002076"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCQ2297474.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(24680..26020)
                     /locus_tag="OHN28_002077"
     CDS             complement(24680..26020)
                     /locus_tag="OHN28_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCQ2297475.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(26022..27374)
                     /gene="gudP"
                     /locus_tag="OHN28_002078"
     CDS             complement(26022..27374)
                     /gene="gudP"
                     /locus_tag="OHN28_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCQ2297476.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(27808..28257)
                     /locus_tag="OHN28_002079"
     CDS             complement(27808..28257)
                     /locus_tag="OHN28_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCQ2297477.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(28275..29057)
                     /gene="truC"
                     /locus_tag="OHN28_002080"
     CDS             complement(28275..29057)
                     /gene="truC"
                     /locus_tag="OHN28_002080"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCQ2297478.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(29057..29386)
                     /locus_tag="OHN28_002081"
     CDS             complement(29057..29386)
                     /locus_tag="OHN28_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCQ2297479.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         7488 a         7597 c         7144 g         7621 t
ORIGIN      
        1 gaatagttat taataagaga aaacagaaac caccgttatg aattagcgca ctaaataacc
       61 gccatcaacc actaataaat gtccgttgac ataattcgat gccggacttg cgaggaatac
      121 ggctgccccc attaaatcct gagtatcacc ccaacggttt gccggaatat gatcaagaac
      181 acgctggtta gtttctggat tactacgggt cgccagcgta atatcggttg cataataacc
      241 aggagcaata ccattcacct gaatattata ttggcctaat tcatcgcaat aggctttggt
      301 gaatccggca agagcatgtt tagtggcaga ataagcaggt gaccattgac cacctaagta
      361 agagaacaat gaacagatat taatgatttt accgcttttc tgcgggatca taatttttgc
      421 cgcttcgtag cttaactcga atgcggcggt caggttcacg tcaatcatcg ggtcccagtc
      481 ggcacgacca aagtccagca ccttattcag cttacaaata ccggcattat tgaccagaat
      541 atcgacggta ccgaagcgct cacagcaggc agcgataatc ttctgcggcg cgccttctgc
      601 ggtgatatcc acctgcataa aatcaacttc aacgccctgt ttttcaatca tttcctttgt
      661 ttcgccgtta tctttgacga aactaggaat aaagacattt gcgccagctt tggccaacgc
      721 catggcaaat gcctggccta aaccgctatt cccgccggta acaattgctg ttttaccttt
      781 cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
      841 tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
      901 cattatctct ggtaactgga gataattttt agcataagga cataactgct aatgtgatcg
      961 taatcacagt gtgatgttcg tattgtcaaa taatttaaaa aagcagaagc gtgattactc
     1021 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
     1081 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
     1141 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
     1201 ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc catggcaaaa
     1261 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggcc
     1321 attattttct atgcccctag cggcgttatt gccgataaat tttcacatcg caaaatgatt
     1381 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacata tccaccgctg
     1441 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
     1501 gtgtcgatta aagccgcatc gttgctgggc gatcatagcg agcaagggaa aattatgggc
     1561 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
     1621 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cggtcattat tatctatagt
     1681 gtggtttaca ttctgctggg cattttgtgt tggttttttg ttagcgataa caacaaccta
     1741 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct ggccgttttg
     1801 cgcatcagca ctacctggta ttgcagcatg gtgatttttg gtgtcttcac catctacgcc
     1861 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcggcg
     1921 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggcggc
     1981 attatcacca cctacagtaa agtgaaatcc cctacccgcg tggtccaaat cctttccatc
     2041 atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaacccgca atcggtcgcg
     2101 atggggattg gcttgatttt attactggga ttcacctgtt acgcctcacg cgggctgtac
     2161 tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
     2221 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ctactggcaa
     2281 gacaccctgc ccgctgcaga agcctatcgc aacatgtggc taatgggcat ggcggcgctt
     2341 ggcatggtga tcatctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
     2401 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
     2461 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg tacgatctgg
     2521 aaggtaatgt ggtttgcgaa ggcaaaggct tattgcagcc gatgcacacg ccagatgccg
     2581 ataccgcaga acatcctgac gatgatttat gggcatcact atgttttgcc agtcacgatt
     2641 tgatgagcca gtttgccggg aataaagaag atattgtcgg cattggcctg ggctcaatcc
     2701 gttgctgccg tgcgctattg aaagccgatg gtacgccagc cgcgccgttg attagctggc
     2761 aggatgcacg cgttacacgc ccttacgaac acactaatcc tgacgtggcc tatgtcacct
     2821 ctttttcggg ttatctgaca catcgcttaa ccggcgagtt taaagacaat atcgccaact
     2881 attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat gctacggtaa
     2941 tggaaaagtt taatatcccc cgccagatgc tttttgacgt gcaaatgccg ggcaccattc
     3001 ttgggcatat cacaccacaa gctgcgctgg cgacacattt cccggctggg ctgccggttg
     3061 tttgtaccac cagtgataaa ccggtagaag ctctgggagc cgggttactg gatgatgaaa
     3121 cggcggtaat ttctcttggc acctatatcg cgttgatgat gaacggcaaa gcactgccga
     3181 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
     3241 gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg ttaggcgagt
     3301 cgttaattca ggatgccaaa gcacaggatc tttcaccgga agatttgctc aacaaaaaag
     3361 cgtcttgcgt gccgcctggc tgtaatggtc tgatgacggt gctggactgg ctgaccaatc
     3421 cgtgggaacc gtacaaacgc gggattatga tcggttttga ttccagcatg gattacgcat
     3481 ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaataat tacgacaata
     3541 tgtgtcatga aatgaatcac tttgcaaagc atgtgatcat tactggcggc ggttcgaaca
     3601 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacgc cgaaacgcca
     3661 ttaacggttg tgcaagtctg ggggctgcga tcaatacggc ggtaggtctg gggctatacc
     3721 cggattacac aacggctgtc gataaaatgg ttcgcgtgaa agatatcttc ataccgattg
     3781 agagcaatgc caaacgctac gacgcgatga ataaaggcat tttcaaagac ctaaccaaac
     3841 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgcgg
     3901 attcgatcca gagctggtcg aatgcgtaag tgagataaac agtgccgggt agatgattaa
     3961 tcccggcata ttcacgtttc cactacaaaa taccgggtaa ccactgctga atattgttct
     4021 ctcctgagca gcgtttatgc tttaattgct aaaccgattt aattagttta gcaattagta
     4081 tattggagcc tttatgagca caagagtatg ggtactcggt gatgcggttg ttgatttatt
     4141 acccgaaagc caggggagac tactacagtg tcctggtggg gcgcctgcta atgttgcagt
     4201 cggtatcgca aggctggggg ggaaaagtgc ctttattggc aaagttggcg atgatccttt
     4261 cggtcgcttt atgtatcaaa cactgagtac agaaaatgtt gatacacgtt atatgtctct
     4321 tgatcctcaa catcgcacct caattgtggc tgtaggactt gatgagcaag gagaaagaaa
     4381 ctttaccttt atggtacgcc caagtgccga tcttttttta caacctgatg accttcctgc
     4441 atttgggccg ggtgaatggc tccatctttg ttccattgcg ctcagtgcag aaccttcccg
     4501 aagtaccgca tttctggcta tggagaaaat acgtcaggct ggcggaaaca tcagttttga
     4561 tcccaatatc cgcagcgatc tctggcagag tgaagcgctg ttaaggaaat accttgatcg
     4621 tgcgctttcg ctggcgaata tcgctaaatt gtccgaagaa gagttgttat tcatcagtga
     4681 cgaaagccag gttcagcaag gcgcatattc attagtacaa cgttatccgt tgactttatt
     4741 gcttattaca caaggaaaaa atggcgtact tgtgtatttt caggggcagt ttatccacta
     4801 tcccgccaaa cctgtttctg tcgtcgatac gaccggggca ggagatgctt ttgtcgctgg
     4861 attacttgcc ggtctggctg attctggaat accaacaaat accagacagc ttgaacgaat
     4921 cattgcacaa gctcagattt gtggtgctct ggcgaccacg gctaaaggcg cgatgaccgc
     4981 cttaccccga caacacgatc tcccttcaca atagtgatcc tgctgttgta tctgtaaccc
     5041 gatttgtcgt gcctgtcaca tttactaaat cggtttagcg aaaataattg ctctggctaa
     5101 aaagtcagct ttattatcaa cacctaaacc ggtttagcat tttagcaatt tttataattc
     5161 tctgatggga cgcgacaaat tatgtataaa aaaacaactt tggcagtgtt aattgctttg
     5221 ctgaccggtg ctacaacggt acatgcgcaa acggatatta gcagtatcga atctcgactg
     5281 gcggcgttgg aacaacgttt aaaaaatgcg gaatcccgcg cccaggcggc agaagcaagg
     5341 gccaaaacag ctgaattaca ggttcagaaa ctggctgaaa cacaacaaca aaatcagcta
     5401 acaactcaag aagtagcaca gagaacagtt cagctcgaac agaaatccgc agaaaacagt
     5461 ggttttgagt ttcatggcta tgcccgttcc gggttactga tgaatgatgc cgcttccagt
     5521 agcaaaagtg ggccgtatct gactcccgca ggtgaaactg gtggagctgt tggccgtctg
     5581 ggaaatgaag ccgataccta tgtcgagtta aatgtagaac ataaacaaac actggataac
     5641 ggtgcgacca cacgctttaa agcaatgttg gctgacggac aaagagatta caacgactgg
     5701 actggcggct ccagtaacct gaatatccga caggcttttg ccgaactggg cgcattacca
     5761 agttttaccg gagcattcaa agacagtact gtctgggctg gtaaacgctt tgatcgcgac
     5821 aattttgata ttcactggtt agactccgat gtcgtatttt tagcgggaac gggcggcggt
     5881 atctatgacg taaaatggaa cgagacattc cgcagtaact tttctctcta cggacgtaat
     5941 ttcggcgatc ttgatgatat cgacaataac gttcagaact acatcctcac catgaatcat
     6001 tatgcaggcc ccttccagtt gatggttagc ggattacggg caaaagataa tgatgatcga
     6061 aaagatgcca atggtgatct cattcaaact gatgctgcaa atactggcgt acatgcgtta
     6121 gttggtctgc acaatgacac tttctatggc ctgcgtgaag ggacggcaaa aacagcactg
     6181 ctatatggcc atggcctggg tgcggaagtc aaagggattg gctccgatgg cgctctgctg
     6241 tctgaggcga atacctggcg cttcgcatct tacggcacaa cacctctggg aagcggttgg
     6301 tatgttgcgc cagcaattct cgcacaaagc agtaaagatc gttacgtcaa aggcgatagc
     6361 tacgaatggg tgaccttcaa tacacgtctg atcaaagagg taacacagaa ttttgctctg
     6421 gcctttgagg gtagctatca atatatggat ctgaagccaa aggggtatca aaaccacaac
     6481 gccgtaaacg gcagcttcta taaactcacc tttgctccaa ctctaaaagc taacgatatc
     6541 aataatttct ttagccgtcc ggagcttcgc ctgtttgcca cctggatgga ctggagcagc
     6601 aaacttgatg attttgccag caatgatgct ttcggcagca gtggtttcaa tactggcgga
     6661 gagtggaatt ttggtgtcca aatggaaacc tggttttaat accagacagc ctcacggatg
     6721 tgaggctttt actgaaataa caatgaatta gagggttata tggatttcga taaaatcgcc
     6781 caatcgttgc ttcctttatt aggtggtaag gagaacatcg ccagtgcagc acattgcgcc
     6841 actcgcctac ggctggtact ggttgacgac acacttgccg atcaacatgc cattggccag
     6901 attgatggag tgaaaggttg ttttcgtaat tcagggcaaa tgcaaatcat ctttggcacc
     6961 ggcgtggtta ataaagtcta tgctgccttt attcaggttg caggtattag cgaatccagt
     7021 aaagcagata ccgctcgact cgccgctcaa aaactcaatc cttttcagcg aatagcacgg
     7081 ctactttcta atatttttgt ccccatcatt ccagccattg ttgcttctgg tttattaatg
     7141 gggcttctgg gaatggtgaa aacatatggc tgggtgaatg ccgataatgc gatttatatc
     7201 ctgctggata tgtgcagttc agccgcattt attattctcc ccatccttat tggctttact
     7261 gctgctcgtg aatttggtgg aaatccttat cttggcgcga cattaggagg gattctgact
     7321 cacccggcac tcacaaatgc ctggggcgtt gctgcgggat ttcagacaat gaacttcttc
     7381 ggttttgaaa tcgccatgat tggctatcag ggaacggttt tccccgtgct tctggcagta
     7441 tggtttatga gcatagtgga aaaacagtta cgcaggttta tccctgatgc tctggatctc
     7501 attctgacgc catttctgac tgtcgtcatt tctggcttta tcgctctttt gattattggt
     7561 ccggcaggac gagcgttagg tgacggtatc tctttcgttc tcagcacgct gattgcacac
     7621 gcaggctggt tagcagggtt gttattcggc gggctatatt cagcgattgt tattacgggt
     7681 attcatcata gcttccacgc aattgaagca ggacttttag gtaaccctgc aatcggtgtt
     7741 aatttcctgc tgcctatttg ggcaatggca aacgttgcgc aaggcggtgc atgtctggcg
     7801 gtatggttta aaaccaaaga tacaaaaatt aaagcaatca ccctaccctc ggccttttcc
     7861 gcaatgttag ggattactga agccgctatt tttggtatca accttcgttt tgttaaacct
     7921 tttattgcgg ccttaattgg tggcgctgcc ggcggtgcct gggttgtctc tgttcacgtt
     7981 tatatgactg ccgttggtct gaccgcaata cccggcatgg caatcgttca gccaacatcg
     8041 ttggttaatt atattattgg aatggtgatt gctttcgctg tcgcttttag cctctcttta
     8101 ttactcaaat acaaaacgga cgaggagtaa tgtcgatgcc ttcacaaatg cccgcgattt
     8161 tacaggcagt gatgaaaggt cagtccaaag cactggcaga tgcccactat ccttgttggc
     8221 atctcgctcc agtcaccgga ctaatgaacg atccaaatgg cttttgttgg tctggaggcc
     8281 gctatcatct gttttatcaa tggaatccac tggcttgtga tcataagtac aaatgttggg
     8341 ggcactggag ttcgacggat ttattgcact ggcagcatga accattagcg ctaatgcccg
     8401 ataaagagta cgaccgtaat ggttgttact caggaagcgc ggttaataat cagggcgttc
     8461 tcacactttg ctataccggt aatgttaagt ttgatgatgg cagcagaaca gcatggcaat
     8521 gtctggccac cgaaaataac cagggcgggt ttgataaact aggccctgtc ataccgttac
     8581 ctgatggtta caccggacat gttcgtgatc caaaagtctg gaagcacaac agccaatggt
     8641 acatggtgct tggcgcgcaa gataaagaga aacgggggaa agtactgctg tattcctctg
     8701 tcgatctcta tacctggtct tttcatggag aaattgctgg taatggtctg aatgaaatag
     8761 ataacgcagg atacatgtgg gaatgtcctg atcttttcgc ccttgatggt gaatacattc
     8821 tcctctgctg cccccaggga atggcgcgcg aacatgagcg ctatctgaat acttatccct
     8881 gcgcctggct acatggacag tttgattacg agacaggcaa gtttacgcat ggcgctttct
     8941 cagagctgga tgcgggattt gaattctatg caccacagac gatggaagcc ccggacgggc
     9001 gacgattgct ggtcggctgg atgggggttc ctgatggcga agagatgttg caaccaacca
     9061 gaaagcatca ttggcagcat caaatgacct gttttcgcga actctcattc cagaagggaa
     9121 aattgtttca gatgccaatc agggaactcg cgcaactgcg tgaagctgaa cacttctggc
     9181 aaggaaaagc ggatcatgcg ccgcatgtcg aaattgaacg cctggaaatg gacatcattc
     9241 cgtcgggtga gctgtatctg aattttggca atgcgctggc actgcatctt aatgacgatg
     9301 gtattcaact acagaggaga agccttgcag ggcaagagaa actaactcgt tactggcgag
     9361 gaagcgtaac gtcactgaag attctctgtg acagttccag cgtagagata ttcatcaata
     9421 atggtgaagg tgtaatgagt aatcgttatt ttccccatca tccagcttcg ctgatattac
     9481 agggtgagtc tgacgtcacg ttacgctact ggtcgctacg cgcctgcatg gtagaatgag
     9541 ggttttgaat tacggatcct cgtcgtgaga aaaaccaaac gcgttactat taaagacatt
     9601 gctgaactgg cgggagtgtc gaaagcaacc gccagtctgg ttctcaatgg tcgtggcaaa
     9661 gagttacgtg ttgcgcagga aacgcgtgag cgtgtgctga gtattgccca acagcagcat
     9721 tatcagccca gcattcatgc ccgctcatta cgggacaacc gcagtcatac cattggtctc
     9781 gtcgttcctg aaatcactaa ctacggattt gcagtatttt cccatgaact ggaaacattg
     9841 tgtcgggaag cgggcgtcca actcctcata tcctgtactg atgaaaatcc ttcacaggaa
     9901 acaatggtgg tgaacaatat gattgcccgc caggttgatg ggttaatcgt cgcatccagt
     9961 atgctgcatg ataatgacta tcaaaaactt agcgagcaat tacctgttgt gttgttcgat
    10021 cgccacatga acggcagcac attgccattg gtcattactg actctgtttc tcccaccgcc
    10081 gctctggtgg cagatattgc acggagtcac ccggatgaat tctattttct ggggggacaa
    10141 ccccgtttgt caccgactcg tgatcgactg gaaggattta cacaaggctt gcagcaagcc
    10201 ggagtaacgc tacaacctga atggattatt cacggtaatt atcatccgag ctcaggctat
    10261 gaaatgtttg ccgcgctatg cgctcgttta ggacgtcccc ctaaagcctt attcactgcc
    10321 gcttgtggat tactggaagg cgttctgcgc tatatgagcc aatataattt actcgacagt
    10381 aaaatccacc tggcgagttt tgatgatcat tacttgtatg attcactttc ggtaagaatc
    10441 gacacaatac aacaggataa tcgccaactt gcttttcact gctttgaact tatttcgcaa
    10501 ctgattgaag gtgaaacgcc atccccatta cagcgttatc ttcctgccag tctacaaaaa
    10561 cgctaccgat aatcccgtca ctatcttgca tcgcccctgt aaaacggggc gattattttc
    10621 atcctgaacg ttaaatatca atgatgcata atcaaatcag gcaatattta aatatttatg
    10681 cgtttgcatc gacaaacgcc agttgcgcgc aatgcaggta tcgatacata aacgcgtagc
    10741 atcttccttc tggctaattg gctgcagtgc aatgactcgc ggtttatcat cggtcagcgt
    10801 cgcaagcaat tcatccagtg cttcgatatc acgtacgcgt cccactgggt gtttgatttc
    10861 gttggctcgc tccagtgcct gtgacaacac ttcatagcca ccgcgcatat tcagctttgg
    10921 cgacacggtg acccaggtat tcggtgtaca gcgtacttca tgagtaccac tggtttctat
    10981 ctggcagcta aaaccgttct tttcgagcag gtcagtcagc ggcagcaaat catgaatgca
    11041 aggctcacca cccgtaatca ccacgtgacg cgcggtgtat ccctgacggc caatgacagc
    11101 cagcaaatct tcactgctcg ccgcccccca cttatcactc tctttggtct tcgccagaat
    11161 gctgaaaagg gagacttccc gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca
    11221 ggcacagcca accggacatc cctgtaaacg aataaaaatg gcggggacgc cggtaaagta
    11281 accctcacct tgcagggtct ggaacatctc gttaatcggg tactgcatag cattctctgt
    11341 gaagtggata attgttaatt attgcagatc ctgccacaac aatcatgtct tattaacatt
    11401 ctgttaccgg caggtttaaa gaaaaatgcg aaacatatcg ttaataataa aagggagtga
    11461 cgtattatgt cagaaagaaa taaagtaaag ggatttaatc atgtcaaaaa cattcaccaa
    11521 aattatattt atcttttctg tattcatttt taattataac gaacctcaaa ttaccgatac
    11581 cgccgtcaat ttatttatcc agctttaata ggacatttat aatgcgctat ttcatactaa
    11641 tattcacact cgtgtgttcc tttgttgcag cccaaccaac tattgtccca caattacagc
    11701 aacaagttac ggatcttacg agtagcttaa actcacaaga aaagaaagaa ctgacgcgca
    11761 agctagaatc tattttcaat aatacccaag tacaaattgc tgtattaatt attcccacaa
    11821 ccaaagacga gacaattgaa caatatgctg caagggtttt tgacaattgg cgtttaggag
    11881 atgccaaacg caatgatggg atactgatca ttgttgcctg gtcggatcgc actgtccgca
    11941 tccaggtagg ttatggactg gaagaaaagg taaccgatgc tctggctgga gatatcatcc
    12001 gtagcaacat gatacccgca tttaaacaac aacaattagc taaaggatta gagttagcta
    12061 taatcgcttt gaataaccga ctcacttcac aacaccaata ttcggttaat ccttcagaaa
    12121 gtgaatcagc atcttccagc gatcattact actttgctat tttttgggta tttgcagtga
    12181 tgttcttccc tttctggttt tttcatcaag gcagtaattt ttgtcgcgca tgtaaaagca
    12241 gtgtctgtat ttcagcaatc tatcttttag atttattcct gttctccgat aaaacttttt
    12301 ccattgctgt atttttcttc ttttttactt tcaccaccat tatggtcttt acctgtttat
    12361 gtgtacttca gaaaaaagca tctggtagaa gttatcattc agacaacagc ggttccggtg
    12421 gcggctcaag tggcggcgga tttagcggcg ggggcggttc ttctggcggt ggcggcgcgt
    12481 ccggtcgttg gtaactcaac cataaaaaac gccagcccga aggctggcat ttttaaatca
    12541 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
    12601 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
    12661 tcatagaacc cgttttgatc tggcctgcag cagtaccaac agccaggtca gcgatggtag
    12721 cgtcttcagt ttcgccagaa cggtgagaga taactgcagt gtagccagcg tctttcgcca
    12781 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
    12841 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
    12901 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ttggtatgcg aaaccgtccc
    12961 agtcagattc gtccagaccg tcttcgatgg aaacgatcgg atactgtttg gtcagttctt
    13021 ccaggaagtg agtgaattct tcagaggtga atgctttgtt gccttcgcca gccagaacgt
    13081 atttaccgtc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
    13141 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
    13201 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
    13261 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
    13321 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
    13381 caccaccgtt gatgatgttc atcatcggaa ccggcataga gtatttgccc ggagtgccgt
    13441 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
    13501 tagccagaga tacagccagg attgcgtttg cgccgaattt ggatttgttt tcagtgccgt
    13561 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
    13621 cctgagcgat cgggccgtta accgcagcaa cagctttggt tacgcctttg cccaggaaac
    13681 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
    13741 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
    13801 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
    13861 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt
    13921 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttta
    13981 caaagccagc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
    14041 ggcaagccac gaaccacgga tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
    14101 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
    14161 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca
    14221 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
    14281 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
    14341 caaccgggta cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa
    14401 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
    14461 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
    14521 aaccgccagg tacgaggatt gcgtccagac ctttgaggat ttcaacgccg cgcgtttcaa
    14581 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
    14641 tcagtgcttc gatcacggat ttgtatgcat ccggcagctc aatgtatttg ccgaccatac
    14701 caatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
    14761 gattcgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
    14821 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
    14881 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgctgga acggcgcgat
    14941 ctgaacgaca aatcaggata tcaggctgga taccgattga aagcagctct tttacagagt
    15001 gctgagtcgg tttggttttg acttcacctg acgctgccat gtacggcacc agcgtcaggt
    15061 gcataaacag ggtgtgctca cggccaattt caacagccat ctggcgaatc gcttcgagga
    15121 acggcaggga ttcgatatca ccgacagtac cgccgatttc gaccagtact acgtcatgac
    15181 cttcgccgcc ttccagcacg cgctctttga tagcgttagt gatgtgcgga ataacctgta
    15241 cggttgcgcc gagatagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
    15301 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
    15361 ggtccaggtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
    15421 tcatagtacc gggatcgacg ttgatgtacg gatccagttt catgatggtc acattgaggc
    15481 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
    15541 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
    15601 aaaagacgat ggaataacca ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca
    15661 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
    15721 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
    15781 atcgcgcttg accacgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
    15841 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
    15901 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc gctttttgca atgcgatttc
    15961 cgcttttgtc cctaaatgac gagccagatt gaccgtggca aacagcaggt cacccatttc
    16021 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
    16081 ctcttcgtag actttatcga ctaccggacc aagcgtcgtc cagtcgaaac caacgttggc
    16141 gcaacgtttc tggatttttt gcgcacgcat taaagccggt aaactacgag gaatatcgtc
    16201 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
    16261 aagcacttca ctactatttt cggcagaact gtcagcaaaa acatgcggat ggcgacgctc
    16321 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
    16381 tatttgtgcg taaaacacca cctggaacag cagatcgccc agttcaccgc gcagatcgtc
    16441 aaaatcttca cgggcgatgg cgtccagcac ctcgtaggtt tcttcaaggg tgtaaggcgc
    16501 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccgggt cgcgcaggcg
    16561 ctgcatgatg gtgagcaaac ggtcgatttg attcattgaa ttatcctgaa aattgcgggt
    16621 ccgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
    16681 gctatgtaag attaactccc gtgcaaccga cgcgcgtcga taacatccgg tacctggttg
    16741 agtttaccca gcacgcgacc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
    16801 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagtacgtt caccttctcg
    16861 ttggcgagaa tggtggtgat atcacgtaac aacccactac gatcattagc caccacgcgg
    16921 accaccagcg aatatccggc ggagtagctc tcgccccata ccgcgtcaac aatgcgttct
    16981 ggcgcatggg agcgcagttc cgccaattgt tcgcaatcgg cgcggtgtac tgaaataccg
    17041 cgcccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
    17101 atgtggtgca tcaggttacc gaccccttct accaccacac gaccgttatc tttactgcgg
    17161 ttttgcggcg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
    17221 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
    17281 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
    17341 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
    17401 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
    17461 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
    17521 ggctgtttct gggtgataat ttcaatctgg tcgcccatct gcagctggta ggtgaacggc
    17581 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
    17641 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
    17701 aagacgtaca ctcggtcgtc aaagacctga ctacgtactt cgtcgagcat ttcgccggaa
    17761 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
    17821 cccgaacgtg cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc
    17881 aactctgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt tttgccaccc
    17941 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
    18001 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
    18061 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
    18121 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acggccatac
    18181 acttcggctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
    18241 atatagtgtt cgcggtcgag acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg
    18301 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
    18361 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
    18421 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
    18481 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
    18541 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
    18601 ccgtgaataa ggttaacgac cgacttaccg acgctctcgc gcagcacatc ttcgctgact
    18661 acgttggcat cagccagagg gaatagcagc gccgcccgca gcgtgtcaat gtccatactt
    18721 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
    18781 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
    18841 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
    18901 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
    18961 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
    19021 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
    19081 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
    19141 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc aacaacactt
    19201 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
    19261 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
    19321 ccggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
    19381 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttccacatcc agccattcca
    19441 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
    19501 taaaagttaa gcgcaaccca tttgagtcat accagggcat ctcaccagag acggtttcga
    19561 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcattcca
    19621 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatt agcgtgccgc
    19681 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccaggc
    19741 atgccctgac tttgggcagc aatgcttcta gttggggcac taaaatgggg cattgtttaa
    19801 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
    19861 gtaagtagtt cagacttaaa cgcgcgcgac ggcgatagcc ccaaggaaca tcggcgatca
    19921 cttcagagac ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
    19981 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
    20041 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
    20101 ttttatcttc agtaacagta acttccgcgt tttcctgcgg caataatccg gggataaata
    20161 atgttttgcc gttatgtcgc gccacaccct gaccaaaaga gtcgaggtcg ttgactgaaa
    20221 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
    20281 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
    20341 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
    20401 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
    20461 gccggtgcca gcattattga gccgcttgca gtttctactg aatatggcat gagtctgcaa
    20521 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc tgatattgtt
    20581 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
    20641 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
    20701 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
    20761 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
    20821 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg
    20881 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
    20941 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
    21001 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
    21061 gcaatgtcgc tggcggctta tcacgaagag atgcaacaca atatcgacca ggcgacgtcc
    21121 gatctgcgtg agacgctgga gcaaatggag attcagaacg ttgagttgga tctggcgaaa
    21181 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
    21241 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
    21301 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
    21361 attattaatg atgttctcga cttctcgaaa ctggaagccg gtaagctgat tctggaaagt
    21421 attccgttcc cactgcgcag cacgctggat gaagtcgtta cccttctggc acactcttcc
    21481 cacgataaag ggctggaatt gacgctcaat attaagagcg acgtccccga taacgtgatt
    21541 ggcgacccgc tacgtttaca gcaaatcatc actaacctgg tggggaacgc gattaaattc
    21601 accgagaatg gcaacatcga tattctggtg gaaaaacgtg cgctgagtaa taccaaagtg
    21661 caaattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    21721 ttattccagg ccttcagaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    21781 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggtgatat ttcgttccat
    21841 agcctgccga atcgtggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    21901 attattatcg aagggccatc cacccagtgc ctcgctggta agcgcctggc ctatgtcgaa
    21961 ccaaactccg cagcagcaca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    22021 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    22081 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    22141 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa
    22201 cttaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    22261 cctgccctga cggaatttta tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    22321 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    22381 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    22441 gttgaacggg cgaaacagat gccgttcgat ttaatcttaa tggatattca aatgccggac
    22501 atggacggca ttcgggcatg tgagctcatc caccagctcc cgcatcagca gcaaacgccg
    22561 gttatcgcgg taacggcgca tgcgatggcc gggcaaaaag agaaactgct tggcgcgggg
    22621 atgagtgatt atctcgccaa accgatcgaa gaagagcgat tgcataattt gctgttgcgc
    22681 tacaagcctg gcagcggtat ttcctctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    22741 gtcaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    22801 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    22861 gttgaggaac agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    22921 catggcagtt gcggctatag cggtgtaccg cgtatgaaga atctctgcca actgatcgaa
    22981 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    23041 gacgagatgg ataatgtagc gcgcgaagcc agcaaaattc tcgggtaatg gacgtcttgt
    23101 cgagtagcaa ccggattttt atgacgctgg tttcagattg gtgacaagtg cctgtgttta
    23161 tgccggatgc ggcgttaacg ccttatccgg cctgcaaaac caattaaatt caatgaatta
    23221 aaaaatgatg tagccctgat acgtagcgca acaggcaatt ttgcgtttgg catcagtctc
    23281 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacggacgc
    23341 ttgttatcga acgtccagcc ggggatcagg tactgcattc ccatcgcatc gtcacgcgcg
    23401 ccaagcccgt gtttctgata cagctcgtgc gctttcatca cttgatccat atcgatttct
    23461 acacccagcc ccggtttttc tggcacctga accagaccgc ctttgatctc taacggttct
    23521 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatcgc ggtgatttta
    23581 cccggtgcgg cagcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttg
    23641 gagtgtgaac cccaggtcag gccaaattca tgacacattt gcgccacacg taccgaacct
    23701 tgcatcgtcc agaaatgcgg atccgcaagc gggatatcaa cggattgcaa cgagagcgta
    23761 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    23821 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    23881 tctgcataag ccagcgaacc tttcaggtat gtaccgattt taatcgcttc gttcagcgac
    23941 caggcaccgt taggatcgag cgtaatacgc gcctgcggga aacgttgcgc cagtgccaca
    24001 atagattctg cttcttcttc cccggccagt acaccgccct tcagtttgaa atcgttgaag
    24061 ccatattttt catatgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    24121 tcgtgacgca ggcgatacca gtcacattga tcatccggct ggctttgata cggcagcggt
    24181 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    24241 tggccatcgc ccagcagcga tgccacgttt acccccagat gctgccccaa cagatccagc
    24301 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    24361 ccgcgcccac cggcatcacg atcggcaaac gtattacgca ccagcgtcag aacgtttttg
    24421 tattcaccca gcgttttacc caccaccagc ggaatcgcat cttccagcgt tttacggatt
    24481 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    24541 atattacgcg tgaagaacgg tgcatgtgca ccactcagat tcatcagcat actgtcatga
    24601 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    24661 tttatgtcct ttttcagaat caatgacggc cgaaaacggg gcgtttacgg tcaaaggtcc
    24721 agccggggat caggtactgc atcgggcctg cgtcgttacg cgcaccgcca ggcagacgtt
    24781 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    24841 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc agacgacaat
    24901 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    24961 ccacatgggt aaacatcgcc agagagatat caaagtggtt attagaatgg cagccccagg
    25021 tcagccccca gtcgtcgcaa agttgcgcca cgcggactgc accggaaagc gtccagaagt
    25081 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    25141 agttggtggc aatcatgttg gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    25201 tcacttcacg accggagaaa ccctgctcag cgccgcacgg gtcttccgca taggtaagaa
    25261 catcattcag ccctttgcac agagaaatgg cttcatcaag cagccaggca ccattgggat
    25321 caacggtaat ccgcgcatcc gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    25381 gctcgccagg taacacgcca cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    25441 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgc cgcaactgat
    25501 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    25561 cgatataaaa cagataaccg agaacggtaa tagcatcacg ttgcttgcct ggccctaaca
    25621 gttcgcagac cggaacattc agcgcctgac ctaacaggtc aagcaaggcg gcttccagcg
    25681 ccgctacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    25741 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    25801 ggcccagcac catcggaata gccccgacca gtgtttgata aatcacctct ccgcccggcg
    25861 cttcaccaat gccggtatgc ccggcattat cggtgagtac cacaatattg cgggtgaaat
    25921 atgcgttatg tgcaccacca atattaagca acatgctgtc atgtccggcg accggaatga
    25981 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    26041 ttcagctcaa tacgcttgat atcgcccacc agcaccaggt agctgagtac cgcgattaag
    26101 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    26161 ccaattgcga ttggcgtgac gatgccagaa atgttaccaa acatgttgaa caggccaccg
    26221 gaaagaccgc tgatctcttt tggcgcagta tcagccatta cagcccaacc cagcgcaccg
    26281 atccccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    26341 tagttgcaga acaccatcac catcgacaac aacatgccca tcacgatcgg tgttttacgc
    26401 gcaatgttca gtgaacccgt gcgacgcatc agccagtcgg aaataatccc ccccaacaca
    26461 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    26521 atcccgcgcg cctgcaccag ataaaccggg aaccaggtaa tgaaaaagta agtcagggcg
    26581 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    26641 atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg atccatattg
    26701 atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacgcc cggatgttga
    26761 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    26821 aagaagaaga cgtgtgacca gcccacttca tgagtcagcc agcccataat cggcgcgaag
    26881 ataaccgttg cgaagtattg ggcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    26941 gccggaaacc aggccgcaac aatgcggctg ttgccaggga aagatggcgc ttcagcaagc
    27001 ccgaccagga agcgcagtgt aaacagggcc acgataatgc cgaatccact aaagatatcg
    27061 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    27121 tttgaaccaa aacgatccag caaccagcca ccagggatct gcccgataac ataagcccat
    27181 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    27241 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    27301 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    27361 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    27421 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt gccgttttta taggtcgttc
    27481 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    27541 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    27601 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    27661 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    27721 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    27781 aacggcattt accgctcgct gaacatatca ggacaacatc gtgccccaat gttcaaccca
    27841 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    27901 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    27961 attcacataa ctactgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    28021 cagggtatct ttaattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    28081 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    28141 aggatcttca aataccgttg ctttgtggcc ctgcgcggtc agaatcgctt ccgcttcttc
    28201 ggccactaac agcgaatttc cgtacatagt gccgacaaaa ataccaattt ccgccatacc
    28261 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    28321 accctttcat tttcaggaag cagaccccgc cagccaaatt gtgataacgc ctgcatccag
    28381 gtgtcgtcca ggcccgcgtg aatagccagc ggctcgccag taaaaggatg cgtcaatgac
    28441 aactgactgg catgcagcat taaccgctga aggccaaaat gctcagcacc gctgcgattc
    28501 tggcgtaaat caccatgttt gctgtcgcca ataatcgggt gccgcaaatg ggcaagatgg
    28561 cgacgaagct ggtgcttgcg tccagttttc ggctccagtt ccaccaggcc gtagcgcgtg
    28621 gtcgggtaac gtccagtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    28681 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    28741 tccaccagtg gataatccag caccgcttct tccatcaacc agccacgcac aatcgcatgg
    28801 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcttcgctg
    28861 gataatccca tcaacaacac accagaagtt ggtcggtcca gacgatgagc agtaaaaaca
    28921 tgctggccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    28981 cagctgcggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    29041 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttctccagct
    29101 cagccagaat aagcgcgcgt tgcggatgat cagtcgccag cgccatttca taatagggcg
    29161 caacagcaaa agcgcccggt aacggctggt tgttatctaa caaatcgtgc attcgcggga
    29221 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    29281 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    29341 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    29401 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    29461 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    29521 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    29581 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    29641 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    29701 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtccttcaga agtgtcatca
    29761 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttctc
    29821 gaagtcttac aaggcgctta cagacaatgc
//
LOCUS       DAJOTB010000048        29715 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJOTB010000048 DAJOTB010000000
VERSION     DAJOTB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.048"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            128..334
                     /locus_tag="OHN28_002082"
     CDS             128..334
                     /locus_tag="OHN28_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCQ2297480.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            435..944
                     /locus_tag="OHN28_002083"
     CDS             435..944
                     /locus_tag="OHN28_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCQ2297481.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            941..2359
                     /gene="phrB"
                     /locus_tag="OHN28_002084"
     CDS             941..2359
                     /gene="phrB"
                     /locus_tag="OHN28_002084"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCQ2297482.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2401..3882)
                     /gene="dtpD"
                     /locus_tag="OHN28_002085"
     CDS             complement(2401..3882)
                     /gene="dtpD"
                     /locus_tag="OHN28_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCQ2297483.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4153..4896
                     /gene="ybgI"
                     /locus_tag="OHN28_002086"
     CDS             4153..4896
                     /gene="ybgI"
                     /locus_tag="OHN28_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCQ2297484.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4919..5575
                     /gene="pxpB"
                     /locus_tag="OHN28_002087"
     CDS             4919..5575
                     /gene="pxpB"
                     /locus_tag="OHN28_002087"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCQ2297485.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5569..6501
                     /gene="pxpC"
                     /locus_tag="OHN28_002088"
     CDS             5569..6501
                     /gene="pxpC"
                     /locus_tag="OHN28_002088"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCQ2297486.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6491..7225
                     /gene="pxpA"
                     /locus_tag="OHN28_002089"
     CDS             6491..7225
                     /gene="pxpA"
                     /locus_tag="OHN28_002089"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCQ2297487.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7261..8052
                     /gene="nei"
                     /locus_tag="OHN28_002090"
     CDS             7261..8052
                     /gene="nei"
                     /locus_tag="OHN28_002090"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCQ2297488.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8049..9095)
                     /locus_tag="OHN28_002091"
     CDS             complement(8049..9095)
                     /locus_tag="OHN28_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCQ2297489.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIIAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9275..9988)
                     /locus_tag="OHN28_002092"
     CDS             complement(9275..9988)
                     /locus_tag="OHN28_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCQ2297490.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            10252..10938
                     /locus_tag="OHN28_002093"
     CDS             10252..10938
                     /locus_tag="OHN28_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HCQ2297491.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            10951..11964
                     /locus_tag="OHN28_002094"
     CDS             10951..11964
                     /locus_tag="OHN28_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCQ2297492.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            11976..12731
                     /locus_tag="OHN28_002095"
     CDS             11976..12731
                     /locus_tag="OHN28_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2297493.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            12746..13057
                     /locus_tag="OHN28_002096"
     CDS             12746..13057
                     /locus_tag="OHN28_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCQ2297494.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            13057..14253
                     /locus_tag="OHN28_002097"
     CDS             13057..14253
                     /locus_tag="OHN28_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCQ2297495.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            14281..15249
                     /locus_tag="OHN28_002098"
     CDS             14281..15249
                     /locus_tag="OHN28_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HCQ2297496.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            15309..15770
                     /locus_tag="OHN28_002099"
     CDS             15309..15770
                     /locus_tag="OHN28_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2297497.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            15770..17080
                     /locus_tag="OHN28_002100"
     CDS             15770..17080
                     /locus_tag="OHN28_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCQ2297498.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
     gene            complement(17127..18410)
                     /gene="gltA"
                     /locus_tag="OHN28_002101"
     CDS             complement(17127..18410)
                     /gene="gltA"
                     /locus_tag="OHN28_002101"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCQ2297499.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSDIKK"
     gene            complement(18616..18723)
                     /locus_tag="OHN28_002102"
     CDS             complement(18616..18723)
                     /locus_tag="OHN28_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297500.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            19104..19508
                     /gene="sdhC"
                     /locus_tag="OHN28_002103"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             19104..19508
                     /gene="sdhC"
                     /locus_tag="OHN28_002103"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2297501.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            19502..19849
                     /gene="sdhD"
                     /locus_tag="OHN28_002104"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             19502..19849
                     /gene="sdhD"
                     /locus_tag="OHN28_002104"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCQ2297502.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            19849..21615
                     /gene="sdhA"
                     /locus_tag="OHN28_002105"
     CDS             19849..21615
                     /gene="sdhA"
                     /locus_tag="OHN28_002105"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCQ2297503.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            21631..22347
                     /gene="sdhB"
                     /locus_tag="OHN28_002106"
     CDS             21631..22347
                     /gene="sdhB"
                     /locus_tag="OHN28_002106"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCQ2297504.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            22709..25510
                     /gene="sucA"
                     /locus_tag="OHN28_002107"
     CDS             22709..25510
                     /gene="sucA"
                     /locus_tag="OHN28_002107"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCQ2297505.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            25525..26742
                     /gene="odhB"
                     /locus_tag="OHN28_002108"
     CDS             25525..26742
                     /gene="odhB"
                     /locus_tag="OHN28_002108"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCQ2297506.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            26836..28002
                     /gene="sucC"
                     /locus_tag="OHN28_002109"
     CDS             26836..28002
                     /gene="sucC"
                     /locus_tag="OHN28_002109"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCQ2297507.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            28002..28871
                     /gene="sucD"
                     /locus_tag="OHN28_002110"
     CDS             28002..28871
                     /gene="sucD"
                     /locus_tag="OHN28_002110"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCQ2297508.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(28975..29697)
                     /gene="mngR"
                     /locus_tag="OHN28_002111"
     CDS             complement(28975..29697)
                     /gene="mngR"
                     /locus_tag="OHN28_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCQ2297509.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
BASE COUNT         7206 a         7230 c         8146 g         7133 t
ORIGIN      
        1 caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaacagc ggtactattt
       61 tcatcagata aacgaattca tttttccggt gctattcacc gggtagatac aaaaggggga
      121 gaaaagtatg gaactctaca aagaatatcc tgcatggctt atctttttac gccgtacttg
      181 tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg
      241 cttttatagc tacctgcacc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga
      301 tcaggccgaa aaggcaaccg gtgattttta ctgattgcca aacatgacgt ttaaagaaaa
      361 ccgccggtgg aaacgctggc ggttttcttt tttcggcgtc tacacttact cctgtaaacc
      421 gttcaggagc agtaatgaat caacaacttt ttgatgacag cacccttatc cgcatttttg
      481 ctttgcatga gttacacaat ctcaaagatc gaggactaac gcgcggggcg cttctcgatt
      541 atcacagccg ctataaactc gtctttctgg cgcattctca gccggagtac cgcaaacttg
      601 gcccgtttgt ggctgatatt caccggtggc aaagtctgga tgactattac aaccagtacc
      661 gccaacgcgt gattgttttg ctttctcacc ctgccaaccg gcgtgatcat actaacgttt
      721 tgatgcacgt tcagggatat tttcgcccac atattgattc cacagaacgc cagcagctgg
      781 cggcgcttat cgacagttat cgccgtggcg agcaatcgct tcttgcgccg ctgatgcgta
      841 tcaaacacta tatggcgctt tatcctgacg cctggctttc agggcagcgt tatttcgaac
      901 tttggccgcg tgtgattaac ttgcgccatg caggagtttt atgactaccc atctggtctg
      961 gtttcgccag gatttacgtc tgcacgataa tctcgcactg gctgccgcct gccgcaattc
     1021 gtctgcacgc gtgctggcgt tgtatatcgc taccccacgc cagtgggcgg cgcataatgt
     1081 gtcgccgcgt caggctgaac ttatcaatac acaacttaat gcgctgcaaa acgcgctggc
     1141 ggaaaaaggt attcctttat tgttccgtga agtggatgac tttgccgcca gcgtcgaaat
     1201 agttaaacag gtgtgcgcgg aaaaccgcgt tactcatctt ttttataact atcagtatga
     1261 ggtaaatgag cgggcgcggg atgtggaagt tgaaagagcg ctgcgtaacg tggtgtgtga
     1321 aggatttgat gacagcgtga tcctgccgcc gggggcggtg atgactggca atcatgagat
     1381 gtacaaagtc tttacgcctt ttaagaatgc ctggctgaaa cggctgcggg aagggatgcc
     1441 ggagtgcgtc gctgcgccaa aagtacgtag tagtggagcg atagatcccg cgccatccat
     1501 tacgctgaat tatcctcgtc agtctttcga tactgcgcat ttcccggtgg aagaaaaagc
     1561 ggcgattgcg caattacgcc agttttgcca gaacggtgcc ggagagtatg agcaacaacg
     1621 agattttccg gcagtagaag gcaccagtcg tttgtccgcc agcctggcaa cgggcgggtt
     1681 atcgcctcgc cagtgtttgc atcgcttgtt ggcggaacag ccgcaggcgc tggacggtgg
     1741 agccggtagt gtctggctta gtgagctgat ctggcgcgag ttctaccgcc atttgatgac
     1801 gtattatcct tcgctgtgta aacatcgtcc gtttattgcc tggacggatc gtgtacagtg
     1861 gcaaagtaat cccgcccatt taaaggcctg gcaggaaggc aaaacgggat acccgattgt
     1921 cgatgctgcc atgcgtcagc ttaacagcac tggctggatg cataaccggc tacggatgat
     1981 tacagccagt tttctggtga aagatttgtt gatcgactgg cgcgaaggcg agcgatattt
     2041 catgtcgcag ctgattgatg gtgatttggc agctaataac ggtggctggc agtgggccgc
     2101 ttctacgggt actgatgcag cgccgtattt tcgtattttc aacccgacaa ctcagggcga
     2161 gaaatttgac cgcgaaggcg aatttatccg ccagtggcta ccggagctgc gcgatgtacc
     2221 ggggaaagcg gtgcatgagc cgtggaagtg ggcagagaaa acaggtgtga cgttggatta
     2281 tccgcaaccg atagtcgatc acaaagaggc aaggctgcga acgctggcag cgtacgaaga
     2341 agcgaggaaa ggagcctgat aaaacgcagc gagcgtttta tcaggcatgg tgctggctta
     2401 ttaagactcc agcgccagcg cgcggtttct gaacttaagc gcctgataca gccaaatcat
     2461 cagtaccagt cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata
     2521 tgcattgatg gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc
     2581 ggcaatcacg cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt
     2641 taatacgccg gtcacgccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc
     2701 gataaacagt tccgcaaagc ccatcaccgc caggcctaac accatcagtg gcagagaaga
     2761 gtgaccatac attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat
     2821 caggccaagg ccaagagcaa attttcccca gatgcgcacg gtacgattac ctgcgacgct
     2881 ttctttaacc acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga
     2941 ctggaacata gcagtcggaa cggtataacc aaacatatcg cggttaacga agcggtcgat
     3001 ataaaggcta atcgagctac ccccctgttg tgcgaatgcc cagaacaaca tgctgaagaa
     3061 ggtgagggta acaatcagcc ccagctcctt acgctgtttt tggttttctg ctttgcgata
     3121 aatttttgcc agaaccccaa ggccaatgat cgtcgcgaca atcaaggcgt ataccgacca
     3181 ctctttccag aacaacacgg taatcagcgc aggcgttgcc accagcagaa ccagtagcca
     3241 tccccagttg ggcaggagaa agtttgtcgc acgcagtacc tttttgttaa cgccgcgggt
     3301 atgtgtgaaa tgacgattgc cacataagaa aatgaccaga cccgcgatca tacccaccgc
     3361 tgccaggcca aagcccatcg cccaactgta ctcttcctgg gcgaaaccac aggcgatagg
     3421 tgcgataata gaccccacgt tacccgccgc atacatcagc gagaaaccgc catcacgacg
     3481 tggatcggtt ggctcataca gctcaccgag caggcaactg acgttggatt taaacagacc
     3541 atagccgcag acgataatcg ccagggacag atagaggaat gacggatgga tctcactggc
     3601 acccagcacc acatgaccga tcgccatcaa caacgccccc agcatcaccg ccatgcgatt
     3661 gccgagaact ttatccgcca aaaagccacc gaggattggc gtgacatata ccagcgaaca
     3721 gtaggcgcta aataactcgt aggcgtgatt atcgctatat tttagttgat tggtgagata
     3781 gaggatcagc agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac
     3841 gacatagtaa atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac
     3901 tgcaacgtca cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat
     3961 acagatctga ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc
     4021 gtgatgatat aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag
     4081 attggctatc gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg
     4141 gaaggcgcga agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc
     4201 gcggcgatta gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gatggtgcaa
     4261 aaaattgtta ccggtgtaac tgccagccag gctttgctcg atgaggcagt gcgtctgggc
     4321 gcggatgcgg tcattgtgca tcatggctac ttctggaaag gtgaatctcc ggtcattcgc
     4381 ggaatgaagc gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg
     4441 catctgcccc tggacgcgca tccggagctg ggtaataacg cacagctggc ggcgttactg
     4501 gggatcacgg tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct
     4561 gtgccgggac tggaactggc ctcctggatt gaagcgcgtc tgggacgtaa gccgttatgg
     4621 tgtggcgata ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg gggtgggcaa
     4681 agttttatcg atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct
     4741 gaacagacca ttcattcagc ccgcgagcag ggattgcatt tttatgctgc gggtcaccat
     4801 gccactgaac gcggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt
     4861 gatgtgacct ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt
     4921 gcaacgagcg cgttgttatc tgataggtga aacggcggta gtactggaac tggaaccgcc
     4981 ggtgacgctg gctagccaga aacggatctg gcgactggca cagcgtctgg tggatatgcc
     5041 gaatgtggtt gaagccattc ctggcatgaa caatatcaca gtgattttgc gtaatcctga
     5101 gtcgctggcg ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gtgaggcgct
     5161 ggagccggag tctcgcttta ttgaaattcc ggtagtttac ggtggcgcag ccggaccgga
     5221 tttggcggtg gtcgcggcgc attgtggatt gagcgaaaaa caggttgttg aattgcactc
     5281 ctccgtggaa tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag
     5341 tttgccggaa caactacaca cgccacggcg cgctgaaccg cgcttgcacg ttccggcagg
     5401 ttctgtcggg atcggcgggc cgcagaccgg tgtttatccg ctggcaacgc cgggtggctg
     5461 gcagttgatt ggtcatacct cactcagcct gtttgatccg gcgtgtgacg aacccatctt
     5521 attacgtccg ggagacagcg tgcgctttgt accgcagaag gagggagtat gctgaagatt
     5581 attcgtgcgg gcatgtacac caccgtgcag gatggcggcc gtcacggctt tcgtcagtct
     5641 ggtatcagcc actgtggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg
     5701 ggtaatgacg ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc
     5761 gaaactgatg ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat
     5821 gccgtctgga ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc
     5881 ccgcagcacg ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta
     5941 atgggatcat gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg
     6001 agggatggtg accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc
     6061 gttaaacagc tgctctgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag
     6121 ttcgatcgcg cctcgcagga tgcattctgg cgttcgccct ggcagcttag cccgcaaagt
     6181 aaccgcatgg gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg
     6241 ttatctcacg gtttgttgcc gggcgtggtg caggtgccgc ataacggcca gccgattgta
     6301 ttgatgaacg acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct
     6361 gatatgtacc atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca
     6421 ctggaagagg cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg
     6481 cggctgcaca atgaaaattg acctgaacgc cgatctgggc gaaggctgcg ccagcgacgc
     6541 agagctatta acgctggttt cctctgccaa tattgcctgt ggatttcatg caggagatgc
     6601 gcaaaccatg caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca
     6661 cccgagtttt cccgacaggg aaaattttgg tcgcagcgcc atgcagctgc cgccagaaac
     6721 cattttcgcc cagacgctgt atcaaattgg cgcgctggca gcgattaccc gtgcgcaagg
     6781 cggcgtaatg tgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc
     6841 acaactggca gacgctatcg ccagagcggt atacgcttgc gatccggcat tgattctcgt
     6901 cgggctggca ggaagcgagc tgattcgtgc aggcgaacga tatggcctgg taacgcgcga
     6961 ggaagtgttt gccgatcgtg gttatcaggc agacggttcg ctggtgccgc gaagccagcc
     7021 aggcgcgttg attgaaaacg aagaacaggc gctggcgcaa acgttggaga tggtgcaaca
     7081 cggcagagtc aaaagtatca ccggcgaatg ggcaatggtc acggcgcaaa cggtctgcct
     7141 acatggcgat ggcgagcacg cactggcgtt cgcccgccga ttacgagcta catttgctga
     7201 aaaggggatt gttgtcgcag cataaccccg cttaataaag aatgaaaaaa ggatatcacc
     7261 atgcctgaag gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc
     7321 aaaccactaa ctgatgtctg gtttgccttc ccgcagttaa aatcttatca atcacgactt
     7381 atcggtcaac acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc
     7441 aacgacttaa cgctctacag ccataatcag ctttacggcg tctggcgcgt ggttgatacc
     7501 ggcgaagagc cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc
     7561 attctgcttt atagcgcctc ggatattgag atgttgactc cggaacaact gaccacgcat
     7621 ccgtttttac aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggta
     7681 aaggaacgat tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat
     7741 caggcgtttc tggcaggact tggcaattat ttgcgggtgg agatcctctg gcaggttggg
     7801 ttgaccggaa atcataaagc gaaagatctc aatgcgccgc aactggatgc actcgcacac
     7861 gcgttactgg atattccgcg actttcttac gctacgcggg ggcaggtgga tgaaaacaaa
     7921 tatcatgggg cgctgtttcg ctttaaggtt tttcatcgtg atggcgaacc ttgcgaacgc
     7981 tgtggcggca tcattgagaa aaccacgctg tcatcgcgcc cgttttactg gtgcccaggc
     8041 tgccagcact aggccgaacg cttcggcgca taggttgaaa taaaccgtgc gatggcaggc
     8101 cccgtcagca aaatactgaa caggcgcagg gtttgcatcg caatgatgag cgccatatcg
     8161 gcattgctcc ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg
     8221 taggcggtca taaaatcaat atgcataaac cgggtcagcc cccacgccat gcccgcacaa
     8281 atagccagca gagcaaaaat cgacagcagg atttgcggta gcgggcgcaa tgcccgcagt
     8341 aatatttgct tatcgaaacc aagaccaatc cgccagccaa ttgccatata tgccatcgcc
     8401 agcagccatt ccgggagttc aatcgtgatg agctgaccag actggagcac tgcgcccgcc
     8461 agcatcggga tgagcatcgt gccggaagga agacgtagca agcatcccgc cgtaccggca
     8521 acgaccgcga gcaaaatcgt aagcaggaga ttaatgctca ctggcgggaa ccagacgatc
     8581 tgctggttaa ccgcttcagc gttatcgccc agcatcatgc gggtgaccag aacagcggct
     8641 cctgcgacga acagcactcg cagatattgc ataaacgcca ccaggcgaat atctgcgccg
     8701 taatcttgcg ccattgcgac cattgccgcc gcgccgccag gagaagaacc ccaggccccg
     8761 gtattcccag gcagtgagct atagcgcacc aataaccaac ccacgatggc gctggaaagc
     8821 agcgtcacca gtaaaatcgc aagcacgatc ggccagttga cggccagcgt ggtgagaatc
     8881 gaaccagtga ggttttgtgc aatcatgcag ccaagaatgg cttgggcagc gagaaaagcg
     8941 gagcggggaa gttgcagagt gactccgtgc atactgaaga tgatccccgc gatcatcggg
     9001 ccaagcagga gtgccgccgg gagatgtaaa gcgaggaagc caatagaaag gagaagtgac
     9061 agaacacata acattcccca ctgtagcact ggcatccgtc actccctgtt taacttacct
     9121 ttggtgtatc gatacagcat aaagaatgcg cctcaacgaa aaaatagcgg taatcaagaa
     9181 aaagacagat tttggctaaa gggcgtatta agtaccgttg tctccctctc gtttgtaaaa
     9241 gagggaagag taaacgattg aactgagata agtcttatga cgaggaatta ttgatcagtg
     9301 cactgaccgg gctgtcgaag tgtgatttca tggcttgagt cgccgtttcg acatccctgg
     9361 cacacacagc atcgcagatt ttctggtgta gtgtgatgat gtgattgcca tactctttct
     9421 gatcgctttg ggcgtttaaa ccctcctgga tggattgccg caactgctga tgtaatgcct
     9481 gcaataacat cataaaaagc ggattttttg aggcaacggc aagtaactga tggaagcgga
     9541 tatcatgggt gataaagctt tcaacatcat tgaactgttc gcggattttt gccatttcag
     9601 cccgtaggtt agcaaaatct tcctcttcgc cattacgggc agcagcttcc acaacgccca
     9661 actccagcca gcggcgtact tgaagaattt gctctgttgt cacttgttcg gtcgccaggg
     9721 caaatccaaa caccacgcgc agcacttcat catcaaattt tttgattcgt ggcactttac
     9781 cgctgtgcgt ttccagaatc cccagggcgg caagtgaacg ataggcctca cgcacaatac
     9841 cccgactgac acccaatttc tgaatcagct gaacttcact gggaactggc atccccggtg
     9901 tcaatttttg ttcggcgatc agattaagaa tatgattttt gacctgtgac gataacgtta
     9961 cgctggtcaa taacgcactc tcactcattt ttactcctgt tgtacttttt cctggtcggt
    10021 gaaatccaga ataccgggac tgtatccttc atattttcct ccagaaccct tgattatcaa
    10081 tagactactg cgcctgtgcg cccgccatgc agtgcgaatc aaagacatat tgtgtgaaag
    10141 agtggcgaag aaatagtaaa tacacaactt tttcgtgacg cccttcgcag tattgtgcgc
    10201 tttttctcct taccctgtaa acctgttaaa caggcttaca ggacgaaaat aatgtggaag
    10261 agtgataacg aactttttgc tttgatgaaa acccggctat ttaccgccgt ggttggcgat
    10321 attctcgaca cgctgggcta tatgcaccag tttgtttctc cggcaatcaa gccgctggat
    10381 gtgaatacgg tggtggcagg ccgggcaatg ccggtgctgg agtcggacgt ttacgaaacc
    10441 aaatcgacgt cgggtaagac ggcgattggc gaaaaaccgt ttggcatcat gtttgaagcg
    10501 ctggatgccc tcaaagagaa cgatgtttac atcactacgg gcagctctct gcgctatgcc
    10561 ttgtggggcg ggttgatgtc tacccgtgcc atgcatctga aagcagcagg tgcggtactc
    10621 gatggttacg cccgtgactc tgctgaaatt cttgaattga aatttcccac tttttgtcat
    10681 ggcacttacg cacaggatca aggtccgcgc ggcaaggtca tcgacttcgg cgtacctatt
    10741 gaatttcacg gcgtgcgagt gaatccgggc gatatcgtgt ttggtgatcg tgacggatta
    10801 ctgattattc cccaggttgt tgagaaagaa gttattaccc aggccctgga aaaagtggca
    10861 actgaaagtg aagtgcgcaa agccatcagt gatggcatga gcacggtaca ggcctttgaa
    10921 accttcggcg taatgtaagg agagcaatga atgaaggcat taaccattga gcagggaagt
    10981 caggcgcgat ttgttgatat ccccgaacct gtctgcggta aaggagaagt gctaatcaat
    11041 gttgagcgtg tcggactgtg tggttctgac ctcaatacct ggcggggact aaatccgctg
    11101 gttaattatc cctgtattcc ggggcatgaa attggcggca cggtgctggc aactggcgat
    11161 atggcagggg cgatcaagcc aggtgaccgg gtggtcgttc tgccttgcac tgagtgtggc
    11221 gtgtgcagct cctgtctgag cggtcgtcct aacgcctgta aaaacaacca aactcttggt
    11281 gtgcaacgtc agggggggat ggcgcaacaa ctgacgatgc ctgcacataa agtcattgtc
    11341 tgtaatgcct taagtgttga tgagttagcg ctggtagaac cgctggccgt gggactacat
    11401 gcggccagac gtgcgcaaat taatgtgggt gaatgggtcg cagttatcgg ttgtggggta
    11461 attggccttg gcgctatcgc cgcggcatcc gccatggggg cgcgcgtaat tgctatcgat
    11521 attgatgatc gcaagcggaa cattgccctg gaatgcggtg cgcaacgttt tcttaattcg
    11581 cagcaatgtg acgtggcggc agaactggtg ctgttaaatg acggtcgcgg cccggaagtg
    11641 gtgattgaag cggtgggtaa tcaacaaacc attgaacagg ctacagggtt ggtggcgttt
    11701 agcggtcgtg tggtttatgt cggttacgcc aaacagccag tgagttatca gagtgcgcag
    11761 ttcctgatga aagaactgga tatccgtggt tcgcgcaatg ccaccgtggc ggattttcgt
    11821 gccgtgctgg cattgatgga gtccgggcgt tatccggtca gcaatctggt gagtgctcac
    11881 tacccgctgg aacaggctga tcacgctttt agcgactggg caagtgatcc cggcgcgtat
    11941 accaaaatcc tcattgacct ttaaggagcg acaccatgaa tgctttttct ttacataaca
    12001 aaaccgcgtt gatcaccggt gcagccagtg ggctggggct ggcaatggcg aaatgcatga
    12061 tagtcagcgg ggcgaaggtc attattgctg acctgaacgg cgagctggcg caagcgaccg
    12121 ccggaacatt ggggtcgcag gccagttgga tacaaatgga tgtcggggat accgcagccg
    12181 cgcaggaaaa agttgatgtg cttattagcg aacacggggc tatcgatatc ctggtcaata
    12241 acgcaggcaa tcactgcaaa aaaccgattg aagagatgag cgtcagtgat tttgagtcag
    12301 tactcgacgt tcacgtagtc ggcgcatttg cgttaaccaa agcgctggtg ccgcatatga
    12361 aacaacgcgg taaaggctgt gtgcttttta ctgcatcgat gacctcattt cttgggcagc
    12421 ctctggttac tggctacagc gcagcgaagt cagcctatct cggcatgatt cgcggtatga
    12481 ccacggagct tgccgttcat gggattcgcg tcaatggtat tgcgccaggc tggatcgata
    12541 caccaatgtt gcgtaaggcc attgaaggtg atgacgaacg ccgtaacaaa atcctgggtc
    12601 gtaccccgat gaaaaaattc ggcaagccgg aggatatcgg ttgggctgcg gtttatctgg
    12661 cttcagacgc agcggcgttt gtcagtggtc aggtgctggt ggtggatggc ggcgcattga
    12721 tcggcttctg atgtacggag gaattatgcg caaacgtgcg ttaaagctac atgccaaaga
    12781 taactgtgta gtcgcgctga atgacatcgc cagtggcgat attgtctggt gggatgaggg
    12841 ggaaatggtg gcctgtagtt ccgttaccct ggggcacaaa ctggcctgcg aagcgctgaa
    12901 caatggtgat tcgattctga aatacggcgc cattatcgga cacgccactc gcgatattgc
    12961 catgggtgaa catgtgcatt cccataatct tgccagcaat gccatcgcca tttttcatca
    13021 tgatgatgcc ggctatcagt cagaggaaaa agcataatgg aaggttatgt acggcaaaat
    13081 ggtcgaccag gtattcgtaa tactgtgcag gtaatcttta tggtggagtg tgccagccat
    13141 gtcgctaaaa agattgccga aggatttcct tgtcacgacg tgcagtattt tggtttttca
    13201 ggttgttatc ccagcgatta tggctatcgg ctggtgaaaa atctggcgac tcatagcaac
    13261 gtaggtggtg tactgattat ttcgctgggg tgcgagaatt tcgatcgccg tcggctggag
    13321 caagaagtac gagaaagtgg gcgaccgtgc catacgctgg tcattcagga aaataaaggc
    13381 acgaccaaca ccattactct gggtaagcag ttggtcgctg aaatgctgga acagcttgcg
    13441 gatacaccgc gttgtatcct gaactggagc gacctggtgg ttggtactat ctgcggtggc
    13501 tccgatggca ccagtggtat cacgggtaac ccggcagtag gacgtgcctt tgaccaactg
    13561 ctggagaaag gggctacctg tatttttgaa gaaagcggtg agttgcttgg ttgtgagcag
    13621 catatgatgt cgcgcgcggc tagttcgcag gcacgtgatg ccatcgaagt ggcaatgaca
    13681 aaagccgaac gttattacag tttgatgggg ctggggagtt tttctaacgg taatgctgaa
    13741 ggcggcttaa ccactcagga agaaaaatcc cttggggctt ataccaagtc gggcgatgcg
    13801 ctgattgctg gcgttatttt gccgacgcaa caaacgccct ttcctgggct ttggttgatg
    13861 gacgtggtgc ctgatggtga acctcgtttt gggtatccga atattaacga ttcctcagaa
    13921 gcactggaac ttatcgcctc tggctgtcat atggtgttat ttaccacagg tcgcggttca
    13981 gttattggtt cagtgatcgc tcctattatt aaagtgtgta ataacccgca gacgtgggag
    14041 catttatctg acgatatgga cgttaatgcc ggtcgtgtaa ttagcgaagg cgcaacgctc
    14101 gacgatattg cagacgatat tatgcaggtg attgaacatg tcgccaacgg tcaatattgt
    14161 gccgcagaac gactggaaca tcaggagttt gttttaacgt ataaagcatt tgattacagc
    14221 aaaagtgcag tctgtcgggc agatccccgt taatcgataa agctcttaca ggtaataata
    14281 atgaaaaaat tactgacacc acttttattt cttttagctt tatttagtcc gatgctgaat
    14341 gccgcaccgg taactatcaa aatcgcctat gaaagtaacc ccggcgagcc agcggataca
    14401 gtagtgaagc actgggcaga acttgtgaag aaaaaaagca atggagatat tgttttcgaa
    14461 ctttacccaa gttcacagtt aggttctaaa caagatatta ctgaacaagc gttgatgggg
    14521 atgaatatta tcaccatcag tgatgtagga tttttaactg actacgatgc agacttggga
    14581 atattgtttg ggccttattt aaccgaagat ccgcagaaac tgtttgcgat ttatgccagt
    14641 gactggttta aacaaaaaga acagaaacta cgggaaaaag gtgtgcatat tgtcatgagc
    14701 aactatctgt acggtaccag gcagatcatt gctaaaaaac cgattcgtac acctgaagac
    14761 ttgaaaggga tgaagatccg cgtgccgaat aacgtcatgc agattaaagc tatcgaagcg
    14821 atgggcgcaa ccccaacacc aatgccgctg ggagatgttt atccggcgct gactcagggg
    14881 atgatcgatg gtgtggaaaa cccggtttct gttctctatg ggcaaaagct gcatgagcag
    14941 gcgaaatatc tgtcaatggt gaactatctg accaatacat cattatggtt gggtggcgaa
    15001 gcgtttttca gtaccctgac acctgcgcag ttagatatta ttcaccagac agcgtatgaa
    15061 gctggtttat atagccagaa aattaccact gagcaggatg cgacaatgtt gaaaaccatg
    15121 caggagcagg gcgtggaaat catctatcct gacgtcgccc ctttcaaaga aaaagcctta
    15181 aaagtctatc aacaattccc ggaatggacc cctggtttgt atgacactat tcagcaacag
    15241 ctgaaataac ttcatggcat aaggactggt cggtgtttcg gcaccgaccg ttaagagcag
    15301 aggtgtttat gcgtctttta gggaaaatga ttgaagttgc tgcggtatta acgctcttta
    15361 tattggtaat catgaccatt gctgcggtaa taatgcgtta ctgttttcat atgccattac
    15421 agtggacaga agaggtttcc ggtttattaa tgatttgggt agtcatgttt ggtgcggttg
    15481 ttgctgaacg agataatacc catttatcga ttccattttt agccgatgca cttacttcac
    15541 gcgggcaacg tatattagcg tttttagtct ctctgatgtc tattattttg ctggtggtca
    15601 tggcctggtc agcatggaaa ttagctgccg ggacagcctt taaaaccacg caaatattaa
    15661 aaatatcctg gttttggatc gatattgccg tcacggttgg cgctatcggt gtcgcctgtt
    15721 ttactgcctt acaactgatc aagaaaacgc cacgcaagga aggtgaataa tgacagcatt
    15781 gagtgaatta ctggcgggaa gctggctgga taactggctg atgatcattc cgcttatgtt
    15841 cgtctgttta tttatcaata tgccggtatg gctggcgatg ttttgcggca ttctgaccta
    15901 cttcctttgc tattcaaatg tgccgttaat ggtggcaatt cagcgtttga ttgccacgac
    15961 gcaaacccct tcgctgttag ctattccgtt ttttatctta ttaggcacgt taatgagcta
    16021 taccggtatt gccgcgcgta ttttgcaaat tgccaacgtg ttgattggca gaatgcgcgg
    16081 tggcctgggt ggggctaata ttctggtcag taccatgatg ggcggtgtca gtgcctcaaa
    16141 tctggcggat gccgcaatgt tatcgcgcat gatggtgccg gaaatggaac agaaaggtta
    16201 ttcccgtggc tttgcggcgg cgataacggc aagtggttcg ctgattacac caattattcc
    16261 accgggtatt gcactgatcc tttacggact ggtggcggat gtctccatcg gctccatgtt
    16321 tatggccggg atcctgccag gtctgctgtg ctccgccatg ttgatcgtta ccgtatggtt
    16381 ggtggctatt cgtcgcggtt taaaaccttc cagcgaacac tggccaaaac cgaaagaagt
    16441 gtcagtggcg ctggtgcagg cctggccagc actgttctta attgttgcgg ttatcggtgg
    16501 tattcgggca aatatcttta cgccaacgga agccggagcg gtagcagtat tattggtaat
    16561 gtttattggt tttgtaattt atcgcgaact gaaaatcagc catgtattta cagcactctc
    16621 tgagacggcg cgttccacgg catcggtcat gttaatcatt atggcgagtg ccgcgctggg
    16681 ctggatattt tcccttgagc gggcaggtat cacggttgcg gagtttgtaa cttctctgac
    16741 cgataataaa tacgtctttt tattgattat caatctattg ctgctggtgc ttggcatgtt
    16801 ccttgaaggt agtgccattc tgctgatatt agtcccatta cttaaacccg tggtggctca
    16861 ttttggcatt gatccggtgc attttggcat cattatgatc ctcaacttat cgttaggaac
    16921 gttatcacca cctgtcggaa cggtgatgct gctggtatgt aataccgtac aggtttctgt
    16981 cgggcaattt atgcgtgaag cggtgccatt actgatcagt cttattttac ttctgttggt
    17041 cattacctat attccggcaa tatctttgct gctggtgtag aattaaaaac cctgcgtgtc
    17101 cacgcagggt tttgtcggtg tcatttttac ttcttaatat ccgacttaaa atcacgctgc
    17161 gcgtagccgg tatacagctg acgcggacgg gcaatcttca taccgtcact gtgcatttcg
    17221 ctccagtggg cgatccagcc aaccgtacgt gccatagcga agatcacggt gaacatagaa
    17281 gacggaatac ccatcgcttt caggatgata ccagagtaga aatcgacgtt cgggtacagt
    17341 tttttctcga taaagtacgg gtcgttcagc gcgatgtttt ccagctccat agccacttcc
    17401 agcaggtcat ctttggtgcc cagctctttc agcacttcat ggcaggtttc acgcattacg
    17461 gtggcgcgcg ggtcgtagtt tttgtacacg cgatgaccga agcccatcag gcggaaagaa
    17521 tcatttttgt ctttcgcacg acgaacaaat tccggaatgt gtttaacgga gctgatttct
    17581 tccagcattt tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca cagtgaagca
    17641 atacctgctg cgatacaggc aaacgggttc gcacccgaag agccagcggt acgcacggtg
    17701 gaggtggagg cgttctgttc atggtcagcg tgcaggatca gaatacggtc catagcacgt
    17761 tccagaatcg gattaacttc atacggttcg cacggtgtgg agaacatcat attcaggaag
    17821 ttaccggcgt aggagagatc gttgcgcggg taaacaaatg gctgaccaat ggaatacttg
    17881 taacacatcg cggccatggt cggcattttt gacagcaggc ggaacgcggc aatttcacga
    17941 tgacgaggat tgttaacatc cagcgagtcg tgatagaacg ctgccagcgc gccggtaata
    18001 ccacacatga ctgccattgg atgcgagtcg cgacggaaag catggaacag acgggtaatc
    18061 tgctcgtgga tcatggtatg acgggtcacc gtagttttaa attcgtcata ctgttcctga
    18121 gtcggttttt caccattcag caggatgtaa caaacttcca ggtagttaga atcggtcgcc
    18181 agctgatcga tcgggaaacc gcggtgcagc aaaatacctt catcaccatc aataaaagta
    18241 attttagatt cgcaggatgc ggttgaagtg aagcctgggt caaaggtgaa cacacctttt
    18301 gaaccgagag tacggatatc aataacatct tgacccagcg tgcctttcag cacatccagt
    18361 tcaacagctg tgtccccgtt gagggtgagt tttgcttttg tatcagccat ttaaggtctc
    18421 cttagcgcct tattgcgtaa gactgccgga acttaaattt gccttcgcac atcaacctgg
    18481 ctttacccgt ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc tgggtacaga
    18541 gctctgggcg cttgcaggta aaggatctat tgatgacgaa taaatggcga atcaagtact
    18601 tagcaatccg aattattaaa cttgtctacc actaataact gtcccgaatg aattggtcaa
    18661 tacttccaca ctgttacata agttaatctt aggtgaaata ccgacttcat aacttttacg
    18721 cattatatgc ttttcctagt aatgtttgta acaactttgt tgaatgattg tcaaattaga
    18781 tgattaaaaa ttaaataaat gttgttatcg tgacgcagat cactgttcgg gataaaaccc
    18841 gacaaactat atgtaggtta attgtaatga ttttgtgaac agcctatact gccgccaggt
    18901 ctccggaaca ccctgcaatc ccgagccacc cagcgttgta acgtgtcgtt ttcgcatctg
    18961 gaagcagtgt tttgcatgac gcgcagttat agaaaggacg ctgtctgacc cgcaagcaga
    19021 ccggaggaag gaaatcccga cgtctcctgg taacagaaag ttaacctctg tgcccgtagt
    19081 ccccagggaa taataagaac agcatgtggg cgttattcat gataagaaat gtgaaaaaac
    19141 aaagacctgt taatctggac ctacagacca tccggttccc cgtcacggcg atagcgtcca
    19201 ttctccatcg cgtttccggt gtgatcacct ttgttgcagt gggcatcctg ctgtggcttc
    19261 tgggtaccag cctctcttcc cctgaaggtt tcgagcaagc ttccgcgatt atgggcagct
    19321 tcttcgtcaa atttatcatg tggggcatcc ttaccgctct ggcatatcac gtcgtcgtag
    19381 gtattcgcca catgatgatg gattttggct atctggaaga aacattcgaa gcgggtaaac
    19441 gctccgccaa aatctccttt gttattactg tcgtgctttc acttctcgca ggagtcctcg
    19501 tatggtaagc aacgcctccg cattaggacg caatggcgta catgatttca tcctcgttcg
    19561 tgctaccgct atcgtcctga cgctctacat catttatatg gtcggttttt tcgctaccag
    19621 tggcgagctg acatatgaag tctggattgg tttcttcgcc tctgcgttca ccaaagtgtt
    19681 caccctgctg gcgctgtttt ctatcttgat ccatgcctgg atcggcatgt ggcaggtgtt
    19741 gaccgactac gttaaaccgc tggccttgcg cctgatgctg caactggtga ttgtcgttgc
    19801 actggtggtt tacgtgattt atggattcgt tgtggtgtgg ggtgtgtgat gaaattgcca
    19861 gtcagagaat ttgatgcagt tgtgattggt gctggcggcg caggtatgcg cgcggcgctg
    19921 caaatttccc agagtggcca gacctgtgcg ctgctctcta aggtcttccc gacccgttcc
    19981 cataccgttt ctgcgcaagg tggtattacc gttgcgctgg gtaataccca tgaagataac
    20041 tgggaatggc atatgtacga caccgtaaaa gggtcggact atatcggtga ccaggacgcg
    20101 attgaatata tgtgtaaaac cgggccggaa gcgattctgg aactggaaca tatgggcctg
    20161 ccgttctcgc gtcttgatga tggtcgtatc tatcaacgtc cgtttggcgg tcagtcgaaa
    20221 aacttcggcg gcgagcaggc ggcacgtact gcggcggcag ctgaccgtac cggtcacgca
    20281 ctgttgcaca cgctttatca gcagaacctg aaaaaccaca ccaccatttt ctccgagtgg
    20341 tatgcgctgg atctggtgaa aaaccaggat ggcgcagtgg tcggttgtac cgcactgtgc
    20401 atcgaaactg gtgaagtggt ttactttaaa gctcgcgcga cagtgctggc gactggcggg
    20461 gcagggcgta tttatcagtc caccaccaac gcccacatta acactggcga cggtgtcggc
    20521 atggctatcc gtgcaggcgt accggtgcag gatatggaaa tgtggcagtt ccacccgacc
    20581 ggtattgccg gtgcgggcgt actggtcacc gaagggtgcc gtggtgaagg cggttatctg
    20641 ctgaacaaac atggcgaacg ctttatggag cgttatgcgc cgaacgccaa agacctggcg
    20701 ggccgtgacg tggtggcgcg ttccatcatg atcgaaatcc gtgaaggtcg cggctgtgat
    20761 ggtccgtggg ggccacacgc aaaactgaaa cttgaccatc tggggaaaga agttctcgaa
    20821 tcccgtctgc cgggtattct ggaactctcc cgtaccttcg ctcacgtcga tccggtgaaa
    20881 gagccgattc cggttatccc aacctgtcac tacatgatgg gcggtattcc gaccaaagtg
    20941 accggtcagg cgctgacggt gaatgagaaa ggcgaagatg tggttgttcc ggggctgttt
    21001 gccgttggtg aaatcgcttg tgtatcggta cacggcgcta accgtctggg cggcaactcg
    21061 ctgctggacc tggtggtatt tggtcgtgcg gcaggtctgc atctgcaaga gtctatcgcc
    21121 gagcagggcg cactgcgcga tgccagcgag tctgatgtag aagcgtctct ggatcgcctg
    21181 aaccgctgga acaataaccg taacggtgaa gatccggtgg cgatccgtaa agcactgcaa
    21241 gaatgtatgc agcataactt ctcggtcttc cgtgaaggtg atgcgatggc gaaagggctt
    21301 gagcagttga aagttatccg cgagcgtctg aaaaatgccc gtctggatga cacttccagc
    21361 gagttcaaca cccagcgcgt tgagtgcctg gaactggata acctgatgga aacggcgtat
    21421 gcaacggctg tttctgccaa cttccgtacc gaaagccgtg gcgcgcatag ccgcttcgac
    21481 ttcccggatc gtgatgatga aaactggctg tgccactccc tgtatctgcc agagtcggaa
    21541 tccatgacgc gccgaagcgt caacatggaa ccgaaactgc gcccggcatt cccgccgaag
    21601 attcgtactt actaatgcgg agacaggaaa atgagactcg agttttcaat ttatcgctat
    21661 aacccggatg ttgatgatgc tccgcgtatg caggattaca ccctggaagc ggaagaaggt
    21721 cgcgacatga tgctgctgga tgcgcttatt cagctgaaag agaaagatcc cagcctgtcg
    21781 ttccgccgct cctgccgtga aggtgtgtgc ggttccgacg gtctgaacat gaacggtaag
    21841 aatggtctgg cctgtattac cccgatttcg gcactcaacc agccgggcaa gaagattgtg
    21901 attcgtccgc tgccaggttt accggtgatc cgcgatttgg tggtagacat gggacaattc
    21961 tatgcgcaat atgagaaaat taagccttac ctgttgaata atggacaaaa tccgccagct
    22021 cgcgagcatt tacagatgcc agagcagcgc gaaaaactcg acgggttgta tgaatgtatt
    22081 ctctgcgcat gttgttcaac ctcttgtccg tctttctggt ggaatcccga taagtttatc
    22141 ggtccggcag gcttgttagc ggcatatcgt ttcctgatcg atagccgtga taccgagact
    22201 gacagccgcc tcgacggttt gagcgatgca ttcagtgtat tccgctgtca cagcatcatg
    22261 aactgcgtca gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg ccatatcaag
    22321 tcgatgttgt tgcaacgtaa tgcgtaaacc gtaggcctga taagacgcac aagcgtcgca
    22381 tcaggcaacc agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa agaaatgcag
    22441 aaaccgtagg cctgataaga cgcgcaagcg tcgcatcagg caaacagcgc cggatgcggc
    22501 gtgaatgcct tatccggcct acaaaagaaa tgcaggaaat ctttaaaaac tgcccctgac
    22561 actaagacag tttttaaagg ttccttagcg gacgcagaaa acacgacaag tccgcaacaa
    22621 gtgaaccccg gcacgcacat cagatatgaa atgtgcgtgg tagtatccac ggcgaagtaa
    22681 gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag cctggttgga
    22741 ctcttcttac ctctctggcg caaaccagag ctggatagaa cagctctatg aagacttctt
    22801 aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt tacctggtac
    22861 gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc gcctggcgaa
    22921 agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga agcaggttaa
    22981 agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga atctcgatcc
    23041 gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc acgatctgac
    23101 cgaagcagac ttccaggaga ccttcaacgt cggttcattt gccagcggca aagaaaccat
    23161 gaaactcggc gagctgctgg aagccctcaa gcaaacctac tgtggcccga ttggtgccga
    23221 gtatatgcac attaccagca ccgaagaaaa acgctggatc caacagcgta tcgagtctgg
    23281 tcgcgcgact ttcaatagcg aagagaaaaa acgcttctta agcgaactga ccgccgctga
    23341 aggccttgaa cgttaccttg gcgcaaaatt ccctggcgca aaacgcttct cgctggaagg
    23401 cggtgacgcg ttaatcccga tgcttaaaga gatgatccgc cacgctggca acagcggcac
    23461 ccgcgaagtg gttctcggga tggcgcaccg tggtcgtctg aacgtgctgg tgaacgtgct
    23521 gggtaaaaaa ccgcaagact tgttcgacga gtttgccggt aaacataaag aacacctcgg
    23581 cacgggcgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg atggcggcct
    23641 ggtgcacctg gcgctggcgt ttaacccgtc tcaccttgag attgtaagcc cggtcgttat
    23701 cggttctgtt cgtgcccgtc tggacagact tgatgagccg agcagcaaca aagtgctgcc
    23761 aatcaccatt catggtgacg ccgcagtgac cgggcagggc gtagttcagg aaaccctgaa
    23821 catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta tcaacaacca
    23881 ggttggcttc accacctcta acccgctgga tgcccgttcg acgccgtact gtactgatat
    23941 cggtaagatg gttcaggcac cgattttcca cgttaatgcg gatgatccgg aagccgttgc
    24001 ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg tcttcatcga
    24061 cctggtatgc taccgccgtc acggccacaa cgaagccgac gagccgagcg caacccagcc
    24121 gctgatgtat cagaaaatca aaaaacatcc gacgccgcgc aaaatctacg ctgacaagct
    24181 ggagcaggaa aaagtcgcga cgctggaaga tgccaccgag atggttaacc tgtaccgcga
    24241 tgcgctggat gctggcgatt gcgttgtagc agagtggcgt ccgatgaaca tgcactcttt
    24301 cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaaca aagttgagat
    24361 gaagcgcctg caggaactgg ctaaacgcat cagcacggtg ccggaagcgg ttgaaatgca
    24421 gtctcgcgtt gccaaaattt atggcgatcg ccaggcgatg gcagccggtg agaaactgtt
    24481 cgactggggc ggcgcggaaa acctcgctta cgccacgttg gttgacgaag gcattccggt
    24541 tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg cggtgatcca
    24601 caaccagtct aacggttcca cttacacgcc gctgcaacat atccataacg gccagggcgc
    24661 gttccgcgtc tgggactctg tactgtctga agaagccgta ctggcgtttg aatacggtta
    24721 tgccaccgca gaaccacgca ccctgaccat ctgggaagca cagttcggtg acttcgccaa
    24781 cggtgcacag gtggttatcg accagttcat ctcctctggc gaacagaaat ggggccggat
    24841 gtgtggcctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg agcactcctc
    24901 cgcgcgtctg gaacgttatc tgcaactttg cgctgagcaa aacatgcagg tttgcgtacc
    24961 ttctaccccg gcacaggttt accacatgct gcgtcgtcag gcgttgcgcg ggatgcgtcg
    25021 tccactggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg tttccagcct
    25081 cgaagaactg gcgaacggca ccttcctgcc agccatcagt gaaatcgacg agcttgatcc
    25141 gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgatc tgctggaaca
    25201 acgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac tctacccgtt
    25261 cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg attttgtctg
    25321 gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc atttccgtga
    25381 agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg cctctccggc
    25441 ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg acgcgctgaa
    25501 cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc tgacctgcct
    25561 gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggcga cgtagtcgta
    25621 cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt accggcatca
    25681 gcagacggca ttctggatgc ggttctggaa gatgaaggta caaccgtaac gtctcgtcag
    25741 atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc caaatctgaa
    25801 gagaaagcgt ccactccggc gcaacgccag caggcgtctc tggaagagca aaacaacgat
    25861 gcgttaagcc cggcgatccg tcgcctgctg gctgaacata acctcgacgc cagcgccatt
    25921 aaaggcacgg gtgtgggtgg tcgtctgacc cgtgaagatg tggaaaaaca tctggcgaaa
    25981 gccccggcga aagagtctgc tccggcagcg gctgctccgg cggcgcaacc ggccctggct
    26041 gcacgtagcg aaaaacgtgt gccgatgact cgcctgcgta agcgtgtggc agagcgtctg
    26101 ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa catgaagccg
    26161 attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg catccgtctg
    26221 ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta cccggaagtg
    26281 aatgcgtcta tcgacggcga tgatgtggtc taccacaact atttcgacgt cagtatggcg
    26341 gtttctacgc cgcgcggcct ggtgacgccg gttctgcgtg atgtcgatac cctcggcatg
    26401 gcagacatcg agaagaaaat taaagagctg gcagttaaag gccgtgacgg caagctgacg
    26461 gttgaagatc tgactggtgg taactttacc atcaccaacg gtggtgtgtt cggttccctg
    26521 atgtctacgc ctatcatcaa cccgccgcag agcgcaattc tgggtatgca cgctatcaaa
    26581 gatcgtccga tggcggtgaa tggccaggtt gagatcctgc cgatgatgta cctggcgctg
    26641 tcctacgatc accgtttgat cgatggtcgc gaatccgtgg gcttcctggt aacgatcaaa
    26701 gagttgctgg aagatccgac gcgtctgctg ctggacgtgt agtagtttaa gcttcacctg
    26761 cactgtagac cgacgtggat agggggtcgg tctacggttt aaaagataac gattactgaa
    26821 ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactttt tgcccgctat
    26881 ggcttaccag caccggtggg ttatgcctgt actactccac gcgaagcaga agaagccgct
    26941 tcaaaaatcg gtgccggtcc gtgggtagtg aaatgtcagg ttcacgctgg tggccgcggt
    27001 aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt tgcagaaaac
    27061 tggctgggca agcgtctggt aacgtatcaa acagatgcca atggccaacc ggttaaccag
    27121 attctggttg aagcagcgac cgatatcgct aaagagctgt atctcggtgc cgttgttgac
    27181 cgtagttccc gtcgcgtggt ctttatggcc tccaccgaag gcggcgtgga aatcgaaaaa
    27241 gtggcggaag aaactccgca cctgatccac aaagttgcgc ttgatccgct gactggcccg
    27301 atgccgtatc agggacgcga gctggcgttt aaactgggtc tggaaggtaa actggttcag
    27361 cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga ccttgcgctt
    27421 atcgaaatca acccgctggt tattaccaaa cagggcgatc tgatttgcct cgacggcaaa
    27481 ctgggcgctg atggcaacgc actgttccgc cagcctgatc tgcgtgaaat gcgtgaccag
    27541 tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta cgttgcgctg
    27601 gacggtaaca tcggttgtat ggttaacggc gcaggtctgg cgatgggtac gatggacatc
    27661 gttaaactgc acggcggcga accggctaac ttcctggacg ttggcggtgg cgcaaccaaa
    27721 gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa agccgttctg
    27781 gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat catcggcgcg
    27841 gtagcagaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa caacgccgaa
    27901 ctcggtgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa aggtctgacg
    27961 gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt ttaattgata
    28021 aaaacaccaa ggttatctgc cagggcttta ctggtagcca ggggactttc cactctgaac
    28081 aggccattgc atacggcact aaaatggttg gcggcgtaac cccaggtaaa ggcggtacta
    28141 cccacctcgg cctgccggtg tttaacaccg tgcgtgaagc tgttgctgcc actggcgcta
    28201 ccgcctctgt tatctacgta ccagcaccgt tctgcaaaga ctccattctg gaagctatcg
    28261 atgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg gatatgctga
    28321 cggtgaaagt gaaactggat gaagcaggcg ttcgtatgat cggcccgaac tgcccaggcg
    28381 ttatcacccc gggggaatgc aaaatcggta tccagccggg tcacattcac aaaccaggta
    28441 aagtgggtat cgtttcccgt tccggtacgc tgacctatga agcggttaaa cagaccacgg
    28501 attacggttt cggtcagtcg acctgtgttg gtatcggcgg cgacccgatc cctggctcta
    28561 actttatcga tattctcgaa atgttcgaaa aagatccgca gactgaagcg atcgtgatga
    28621 tcggtgagat cggcggtagc gctgaagaag aagcggctgc gtacatcaaa gagcacgtca
    28681 ccaagccagt tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa cgtatgggcc
    28741 acgcgggtgc catcattgcc ggtggtaaag gtactgcgga tgagaaattc gctgctctgg
    28801 aagccgcagg cgtgaaaacc gttcgcagcc tggctgatat cggtgaagca ctgaaaactg
    28861 ttctgaaata aatatctgta ataagaaata gccctcgccg cttcccccta caggaagggc
    28921 gaagggctgt cggtttcgac atggttggcc atcttatgat ggcctttttt atgcttatcg
    28981 cggcgatttt cgctgcgcta tcagggtaaa tttatagtca tcagtattaa aagcgttgcg
    29041 gctatattca aacacctggc catcaactaa atatccacgc gatacttttt caagaatcgg
    29101 cttagtctgg ctgatattaa gcagacggct catctcttcc gtgggcatca ggggaattat
    29161 ctcctgttcg ctacgatcga taaccatctt cttcacttct tcaataaagt gatatttcga
    29221 attttccatg acctgccagg tgagatccgg gaataatgca agcggcatcc aggtttcttc
    29281 cagcgccacc ggcttttgct tgcgatagcg cacgcgcttc acatgccaca cacgatcctg
    29341 ctcgctaatt tgcagctgct gctgaagaaa attgtcagcc ggaattactt caaatatcag
    29401 gacttcacta tgtgtttcga cgtgacggtc cgacagtttt tcatcaaaac tggttaactg
    29461 aaaaatatcg taattgaccc gctcttcttt gacgtaagtc ccgctgccct ggatgctttc
    29521 gaggatctgc tgctcgacta actggcgcaa agcctggcgc accgtaaccc ggctgacgcc
    29581 aaactctgtt tgtaatgccg attcagtggg taacgcatcg ccaggtttta gctcgccgca
    29641 cgtaatctgc tcacgaatgc gatcggcaat ctgccggtat aagggtttgt gtcccatttc
    29701 tagtatctca ttaat
//
LOCUS       DAJOTB010000049        29453 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJOTB010000049 DAJOTB010000000
VERSION     DAJOTB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.049"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            31..1614
                     /locus_tag="OHN28_002112"
     CDS             31..1614
                     /locus_tag="OHN28_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2297510.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2066..3919)
                     /gene="asmA"
                     /locus_tag="OHN28_002113"
     CDS             complement(2066..3919)
                     /gene="asmA"
                     /locus_tag="OHN28_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCQ2297511.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3941..4522)
                     /gene="dcd"
                     /locus_tag="OHN28_002114"
     CDS             complement(3941..4522)
                     /gene="dcd"
                     /locus_tag="OHN28_002114"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCQ2297512.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4614..5255)
                     /gene="udk"
                     /locus_tag="OHN28_002115"
     CDS             complement(4614..5255)
                     /gene="udk"
                     /locus_tag="OHN28_002115"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCQ2297513.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5573..8890
                     /locus_tag="OHN28_002116"
     CDS             5573..8890
                     /locus_tag="OHN28_002116"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2297514.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASVGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     SPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8997..9845)
                     /gene="alkA"
                     /locus_tag="OHN28_002117"
     CDS             complement(8997..9845)
                     /gene="alkA"
                     /locus_tag="OHN28_002117"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCQ2297515.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSVGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9979..11331
                     /gene="yegD"
                     /locus_tag="OHN28_002118"
     CDS             9979..11331
                     /gene="yegD"
                     /locus_tag="OHN28_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2297516.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11344..13284)
                     /gene="yegI"
                     /locus_tag="OHN28_002119"
     CDS             complement(11344..13284)
                     /gene="yegI"
                     /locus_tag="OHN28_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCQ2297517.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTS
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPSGLLRREYI
                     ILIEIALSGLSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKVEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRVMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13281..14042)
                     /gene="pphC"
                     /locus_tag="OHN28_002120"
     CDS             complement(13281..14042)
                     /gene="pphC"
                     /locus_tag="OHN28_002120"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCQ2297518.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQIQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLALNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14039..14698)
                     /locus_tag="OHN28_002121"
     CDS             complement(14039..14698)
                     /locus_tag="OHN28_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2297519.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGSAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14916..14972)
                     /locus_tag="OHN28_002122"
     CDS             complement(14916..14972)
                     /locus_tag="OHN28_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2297520.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(15244..15300)
                     /locus_tag="OHN28_002123"
     CDS             complement(15244..15300)
                     /locus_tag="OHN28_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2297521.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15579..16826
                     /gene="mdtA"
                     /locus_tag="OHN28_002124"
     CDS             15579..16826
                     /gene="mdtA"
                     /locus_tag="OHN28_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCQ2297522.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKAASREDTKKGARS"
     gene            16826..19948
                     /gene="mdtB"
                     /locus_tag="OHN28_002125"
     CDS             16826..19948
                     /gene="mdtB"
                     /locus_tag="OHN28_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCQ2297523.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19949..23026
                     /gene="mdtC"
                     /locus_tag="OHN28_002126"
     CDS             19949..23026
                     /gene="mdtC"
                     /locus_tag="OHN28_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCQ2297524.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            23027..24441
                     /locus_tag="OHN28_002127"
                     /pseudo
     CDS             23027..24441
                     /locus_tag="OHN28_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            24438..25841
                     /gene="baeS"
                     /locus_tag="OHN28_002128"
     CDS             24438..25841
                     /gene="baeS"
                     /locus_tag="OHN28_002128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCQ2297525.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25838..26560
                     /gene="baeR"
                     /locus_tag="OHN28_002129"
     CDS             25838..26560
                     /gene="baeR"
                     /locus_tag="OHN28_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCQ2297526.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26740..27072
                     /locus_tag="OHN28_002130"
     CDS             26740..27072
                     /locus_tag="OHN28_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCQ2297527.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27219..28580
                     /gene="yegQ"
                     /locus_tag="OHN28_002131"
     CDS             27219..28580
                     /gene="yegQ"
                     /locus_tag="OHN28_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCQ2297528.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29080..29397)
                     /locus_tag="OHN28_002132"
     CDS             complement(29080..29397)
                     /locus_tag="OHN28_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297529.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6807 a         7888 c         7761 g         6997 t
ORIGIN      
        1 gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg
       61 gctgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt
      121 attgccatcc tcgccgaaaa actgccgccg aagcagcgcg accgcgcacg ggttaccggg
      181 ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt caatctcctg gctggtcacc
      241 ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga tttaatcatg
      301 ctgtttggtg gtttcttcct gctgttcaaa gccacgatgg agctgaacga acggctggaa
      361 gggaaagaca gcaataaccc cacgcaacgc aaaggtgcga agttctgggg ggtggtgaca
      421 caaattgtgg tactggacgc catcttctca ctcgactcgg tgattaccgc cgtcgggatg
      481 gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg
      541 gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgccta
      601 agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg
      661 aaaggctacc tgtacgctgc tattggtttc tcggtgatga tcgaggcgct caatcagttg
      721 gctatcttta accggcgacg ttttctttcc gctaatcaga cgctgcgcca gcggactacc
      781 gaagcggtaa tgcgcctgct tagtgggcaa aaagaagatg cggaactgga cgccgaaacc
      841 gcgtctatgc tggtggatca tggtaaccag caaatcttta atccgcagga acggcggatg
      901 attgagcggg tacttaatct taaccagcgt accgtcagca gcattatgac gtcgcgccac
      961 gatattgagc atatcgatct caacgcgccg gaagacgaga tccgccagtt actggagcga
     1021 aatcagcata cgcggcttgt cgttactgat ggcgatgacg cagaagattt gctcggtgtt
     1081 gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa cctgcgggtg
     1141 ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttacctgc cctggagcag
     1201 ttccgtaatg cccgcacaca ctttgctttt gtggtggatg agtttggctc ggtggaaggg
     1261 attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa
     1321 gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt
     1381 catatgccgc tggaagatct ggtgcaatat gtgccgttgc cgctggatga aaaacgtgaa
     1441 tatcacacca ttgccgggct attgatggaa tatttgcaac gtattccaaa acctggcgaa
     1501 gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag
     1561 aaggtgcaga ttattccgct gcgtaaggat ggcgagatgg agtacgaggt gtgatggcat
     1621 cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag cgttgtctgt
     1681 ggtacatgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa tcgtttcctg
     1741 taggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc
     1801 gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc ggcgatttac
     1861 cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac gttcaccggc
     1921 ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg atcttacata
     1981 ttcagaatat aatggtgccg ccacgaacgc ctgatgcgac gctttacgcg tcttatcagg
     2041 cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc acatctttgc
     2101 cattgcggga atctttattc cgctcggccc agtcgttaag acggcgtttt gcttcatcct
     2161 gtagatgttt acgcagtaac tgatccactt gcaggctgta attaagttgc tgccagttgc
     2221 cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg ctttccccgt
     2281 tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct gccagattca
     2341 acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg tttaacgtca
     2401 cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagacgcgtc acgttatcga
     2461 agttttcagc ggccttcaca tcaccaccat tacgttctac cgcttgctga atcatctgct
     2521 ggaagttcat cccttccatg cgtgtatcag tcatttcgac atgtgcctgt ccttgccagt
     2581 tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc agtgacatct
     2641 ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtgccaatc tcaacgtttt
     2701 ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccaac gtgcccggca
     2761 gtgaaacctg tccaccgtta agtttgccct gcagttgagt aatttccagc aaacccgact
     2821 tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc acgttactgg
     2881 cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg ataggccggt tcatcaatac
     2941 gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccttgctgc gcggcaccgt
     3001 tctcaccgtt cgctgtttca ttaaggggga tgaggttgtc aagattcagt tgcgggaatt
     3061 gcagtctcag ctgccattcc ggtttctctg tcaacgtgac ctgtgcttgc ccgctcagcg
     3121 tactgtcatt ggcggtcaaa ctgatttggt taaatgaaag gcgtttatgt gactcctgcc
     3181 actgggcctg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg gccccctgca
     3241 actgccagtt aatttgttca atagccgccg ttaaatcatg cggataatca gaagcatcta
     3301 ctgtaccgtt aagggataat gtgagatcgc gctgatcgcg attaacccgc ccggaaaact
     3361 caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg cggattgtca
     3421 cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta agactggata
     3481 tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg cgcggtgcaa
     3541 ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc actgcccctt
     3601 ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc gccacgtcca
     3661 gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg agagacattc
     3721 gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg tcgagctgca
     3781 attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa tcattcggat
     3841 tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaggagt atcatcagcg
     3901 tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta tcgattcggc
     3961 tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg taaggtcgcg
     4021 ccgccgggcc ggagagcggc tcaaagctca gcgcaccaat taacatgccc ggacgcagcg
     4081 ccagcggcag cttaccggag ttgtaaaact ccagcacaat gcagccagac cagcccggat
     4141 cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccagtgaa gaacgcccgt
     4201 ccagccagcc caccagatcg gctggcagcg tcaccgactc cagcgtcacc gccagcgcca
     4261 gctctcctgg gtgaagataa aacgcctcgc cctcgtcgag cacgatttca tcgctcatca
     4321 cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata aacgctgccg
     4381 tgtgaccacg gaaggtacga aatttattgc ccaggcgcac atccaccgtc gcgccgttaa
     4441 tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc caggcttcaa
     4501 tatctcggtc acacagacgc atggcatttt ctcctttcga gcatcactcc cttaacgcca
     4561 attacgtcaa gggcatacca ggttatcact gaacggtaca caatttatca agcttattca
     4621 aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt cccgccgcgc
     4681 ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa catcgggcgc
     4741 acggtttttt gatattgcgc catcactgaa tccattgaac gcccacgctc gttaacgtca
     4801 cgcttgatgc ggcgcatcag acagatatcc agcggggtat caacgaaaat ggagaagtta
     4861 agttcgtcac gtaaacgcgc atccgtcagc aacaaaatgc cttcgagaat gatgaccttc
     4921 ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct gtaaaccggc
     4981 aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcagcag gctgtgatcc
     5041 atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag atggctttga
     5101 tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac ttgctcacgt
     5161 aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc gccagcgata
     5221 ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg attaacctgg
     5281 atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt catccgcctg
     5341 ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacgaag aattcccggt
     5401 ggcaaattac gttgatcagt tttatacaag gtaaaaaatg ttatacgcag ttgcgcaaat
     5461 tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac ttttgtacta
     5521 gcatgaacac agaaacgaat actgacgacc tggccttgcg gataaagcgg taatgagcaa
     5581 acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg tcagtttagg
     5641 tttagtctcg tttatcttca cccttttctc acttgagctt tcgcagtttg gcacccaact
     5701 cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc atgccgggcg
     5761 catgtggccg ggaattgcgc tgagctgctc gctgggaaat atcgccgcat ccatcctgct
     5821 tttttccacc agctcgctga acatgacctg gacgaccatc aatattgttg aagccgtggt
     5881 cggggcagtg ctgctacgta aattgctgcc gtggtataac cccttgcaaa atctggctga
     5941 ctggctgcgt ctggcactcg gcagcgccat tattccgcct ctgttggggg gtgttctggt
     6001 tgtcctgctg acgcccggag acgatcctct cagggcattt ttgatatggg tactgtcaga
     6061 atccatcggc gctctggcac tggtgccgct gggattgtta tttaaaccac actatctgct
     6121 gcgccatcgc aacccaaggt tgctttttga gtcgctgctc acgttagcta tcacgctgac
     6181 gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt actttcatta ttgtgctgtt
     6241 gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt ttgatcttcc ttaccacagt
     6301 gatgatggta tcgctgatga tggccgcgga tccctctctg cttgctacgc cgcgtacgta
     6361 cctgatgagt catatgccgt ggctaccgtt tttgctgatc ctgctgcccg ccaacatcat
     6421 gaccatggtg atgtatgcct ttcgtgcgga acgcaaacac atttccgaaa gcgaaacccg
     6481 ctttcggaac gcgatggaat attccgccat cggcatggca ttagtgggca ccgagggaca
     6541 atggctgcaa tccaacaaag cgctctgcca gtttctcggt tacagtcagg aagaactgcg
     6601 cggactcacc tttcagcaac tgacctggcc ggaggatctc aataaagatc tgcaacaggt
     6661 tgaaaagctg atcagcggtg aaataaacac ctattcaatg gaaaaacgct actacaaccg
     6721 caatggcgat gttgtctggg cgttgcttgc cgtctcactg gtgcgccaca cggatggcac
     6781 gccgctctat tttatcgctc agattgaaga cattaacgag ctaaaacgca ccgaacaggt
     6841 gaaccagcaa ctgatggagc gcatcacgct ggctaacgaa gcgggcggga ttggcatctg
     6901 ggagtgggag ctaaagccga atatttttag ctgggataag cggatgttcg agctgtatga
     6961 aattcctccg catatcaaac cgaactggca ggtgtggtac gagtgcgtgc tgccggaaga
     7021 tcgccagcac gccgaaaaag tgattcgcga ttcattgcaa tcacgctctc cctttaaact
     7081 ggaatttcgc attaccgtga aagacggcat tcgccatatc cgcgccctcg ctaaccgggt
     7141 actgaataaa gaaggcgaag tcgaacgcct gctcggcatt aatatggata tgaccgaggt
     7201 taaacagctt aacgaggcat tgtttcagga aaaagagcgc ctgcacatta cgctggattc
     7261 catcggtgaa gccgtggtct gtattgatat ggcgatgaaa attaccttta tgaatccggt
     7321 ggcggagaag atgagcggct ggacgcagga agaagcgtta ggtgttccgc tcctgacggt
     7381 gttgcatatt acttttggcg acaacggacc attaatggag aacatttaca gtgccgacac
     7441 ctcacgttcc gcgattgaac aagatgtggt gttgcactgc cgaagcggcg gcagctacga
     7501 cgtgcattac agtattacgc cgttaagtac tctggacggc agcaatattg gttcggttct
     7561 ggtgattcag gacgtcaccg aatcgcgcaa aatgctgcgc cagctgagct acagcgcctc
     7621 ccatgatgca ctgacgcatc tcgccaatcg agccagtttt gagaagcaac tacgcatcct
     7681 gctgcaaacg gtaaacagta cacatcagcg acatgcactg gtgtttatcg atcttgatcg
     7741 ctttaaagcg gtgaatgaca gcgccgggca cgccgctggc gacgctttac tgcgcgaact
     7801 ggcgtcattg atgctgagta tgctgcgctc cagcgacgtg ctggcgcgac tcggtggcga
     7861 tgaatttggt ctgctgctgc cagattgtaa tgtcgaaagt gcgcgtttta tcgctacacg
     7921 cattatcagc gccgtgaatg actatcactt tatctgggaa ggacgtgtac atcgggtagg
     7981 tgccagtgta gggattacct tgattgatga caacaatcat caggcggctg aagtgatgtc
     8041 gcaggctgat atcgcctgtt atgcctccaa aaatggtggc cggggccggg tgacgattta
     8101 cgaaccgcag caagttgccg cacatagcga acgggctgtg atgtcgcttg atgaacagtg
     8161 gcggatgatt aaagagaatc agttgatgat gatcgcccac ggtgtcgctt cgccgcggat
     8221 cccgcaagcg cgtaatttgt ggctgatttc acttaagctc tggagttgcg aaggcgagat
     8281 tattgatgaa caaacatttc gtcgtagctt cagcgatccg gcacttagcc atgctcttga
     8341 ccgacgggta ttccacgatt ttttccagca ggccgcaaaa gcgattgcca gtaaaggctt
     8401 aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc gccacgctgg tgaatgaact
     8461 aattgagctg ctggaaaata gccctctatc accacggtta ttacatctga ttattccggc
     8521 agacgcgatt ttagatcacg cagaaagcgt gcaaaaactg cggctggcgg gatgtcggat
     8581 cgtattcagt caggtgggcc gcgatctgca aatcttcaac tcgctgaaag cgaatatggc
     8641 agattacctg ctacttgatg gtgagttatg cgccaacgtg cagggaaatt tgatggatga
     8701 gatgctgatt acgatcattc aggggcacgc tcagcggctc gggatgaaga ccatcgccgg
     8761 gccagtcgtt ttacccttag tgatggatac gctttctggc atcggcgtcg atctgattta
     8821 tggcgatgtg attgccgatg cccaaccgct ggatttgctg gtgaatagca gttatttcgc
     8881 gattaactaa gggagcgttt gcccattgcc tgatgcgacg ctaacgcgtc ttatcaggcc
     8941 tacaaatcgc tcattcccca ggccggataa gctcgcaccg catccggcaa ccaacgtcat
     9001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acaacgcata agaacgccag
     9061 ggcttccagc gttcggcata acggcggatt tgtgccggtg tcattcccgg aaatcgctgt
     9121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccacg caaagcaaaa
     9181 taatttgccg tccagcgccc gatacccgga aaggtttgca gcgttttcat cgcttgttct
     9241 acgtcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     9301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctact
     9361 gccagccgct gaggcgtggg gaagcagaca taatccggaa aatcatccag ccgttcgcca
     9421 taaagctgtg ccactctgga ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     9481 aaaatcgccc gcacgccctg ctcaaaagcg tcaacacaac caggtaaacg caatccgggc
     9541 cgccccgcgc ctaactttcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     9601 aacaggcggc tcattttcgc cagacactca gcggcaacag gttctaaacc tacacttaaa
     9661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     9721 cctaccgcca gactacgggc ataataactg tccgcgaccg tttccacgcc gctcaccgca
     9781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcagggta
     9841 tacatcgcat ctccttgtta atccgctttc agcataaacg ttattcagac gttacgcttt
     9901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     9961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
    10021 gcggtcatgc gcgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
    10081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
    10141 gatgttccgg cagacgacga tgaaacgcag gcgctgttgc gtcgggcgat tcgttataac
    10201 cgcgaagaag atatcgatgt cacggcgaaa agcgtgcagt tcggtctttc ttcactggca
    10261 caatacattg atgatccgga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
    10321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
    10381 ttgcacattc gccaacaggc gcaggcgcag ctgccggagg caattactca ggcggtgatt
    10441 ggtcgcccga tcaacttcca ggggctgggc ggtgatgaag caaacaccca ggcgcaaggg
    10501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
    10561 gtcgcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgagt gctggtagtg
    10621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca atggcgttcg
    10681 cgtctcgatc gtgaagccag cctcctgggt catagtggtt gccgtattgg tggtaacgat
    10741 ctggatatcg cactggcgtt taagaacctg atgccgttac tgggcatggg gggcgaaacc
    10801 gaaaaaggca tcgccctgcc gattctgccg tggtggaatg cggttgccat caacgacgta
    10861 cctgcgcaaa gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
    10921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
    10981 tatcgcctgg tacgtagcgc agaagagagc aaaattgcgc tttcaagcgt agcggaaacc
    11041 cgcgcctcac tgccgtttat cagcgatgaa ctggctacgc tgattagcca gcaagggctg
    11101 gaaagcgccc tcaaccagcc actggcgcgg attctggaac aggtgcaact ggcgctggat
    11161 aacgcccagg aaaaaccgga tgttatctat ctgaccggcg gtagcgcccg ttcaccgctg
    11221 attaaaaaag cgctgacaga acagttgccg ggcattccga ttgcaggtgg cgatgacttt
    11281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta attgaaaact
    11341 ggctcagcaa cggcttaaat cagcctgcgc ctgagccagt ttttctgccg cctgacgtaa
    11401 cggttccatc aacggcatgg tccgtgctgg agcttgaagg cggaatcgct gcaactctgt
    11461 cgcaccgaca gtcagcgccg attccagccg atgtcgtttg gcggccattt ttgccattac
    11521 cgcttgtctg tctgcaggcg ttaccgcttc gttcggcctg aaaacaaaac ggcgttcgca
    11581 actcgctttc cagtcgataa ccgcctgggt cagatgatca ccaaaacccc taacttgctt
    11641 aacgctccgt cgggtaacgt ccgctgctgt ttcaatacca aaggaccgta acgccgcttt
    11701 acgcgcaggg ccaacgccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt
    11761 ctgcttctgc cgttcccttg cggtgtcgtg aagtgctgcc agatcgcgct tttcttcttc
    11821 cggtaaccca agaatttcgt ccttcatttt ttcgagcatc acccgtttgg cgataaaacc
    11881 ttccagcccg cccaactgtt ggatctggct gactaaatgg tcatagtcca tttttgcgcg
    11941 attaaaagcc tctcttcgtc gctggacttc tactttgtac gctttgcttg tcaggctgcc
    12001 aataatccag atagccgcca gcacgggaac caaaatgata taacgcggtt ctgtctgaag
    12061 gccgcaaagc agcagcgata accctgacag tgcgatctca atcagaatga tgtattcgcg
    12121 ccttaacagg cctgaaggaa gcggcctgcc agcagcctgg aaatgatcgg gtaatggcaa
    12181 ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagactttcg ccagcacaaa
    12241 attcccgccg gtggtaatga cctcttcgcc gagatcaata aaatagataa caccttgatt
    12301 atccagcgca caccatgggc agtcagccaa atgaccggga taaacatgca ttgccgaaac
    12361 ggtacatttc tttaactgtt ggcgtagtaa atcaagtgcc gctacccacg cttttgccgt
    12421 cggacgcccg gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc
    12481 gcccggtaac atcgacagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt
    12541 atctgacgcg taggcataac ggaaatgggc aatatccgtc tccagcgcat tacccgcatc
    12601 agagataagc ggtacaccgg aataaggatg ccgcccacca aacaagacgt gaaatatcag
    12661 caacgcaagg ccaaaattat cgtgattttc tgtgcgttca aagccgacaa atgatggcat
    12721 ggtttgcagc tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtacc
    12781 attggcatta atctgaaagg agtcgctatc aatcatcacc actttgctgt cgcgacctac
    12841 cataaagctg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa ccgtagcaaa
    12901 agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaact
    12961 ctggcgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg
    13021 catcataaaa ccgataactt ttccacctcg tccaccgtga agcgttgcct gcggccaggc
    13081 gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt
    13141 taaggcggga ggcgacgtgt gataaatctt ggcgacgctc tcgacaaact cctcgatatc
    13201 ataaaccgca ccttcgccgc ctttgcccag ttcacggcct ggcgtaacgc actcaccagt
    13261 agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca
    13321 tccgtacgct cgtttaccgc cgggctggac aaaaactgct ttaacaattc aggtaataaa
    13381 tcgagttgtt cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa gaaaggcata
    13441 tgcggagaat tatccagcat atttaacgcc agtcgctgaa taccatccgt aaatgctgca
    13501 actttgtgcg cacgctcagt gctggtgaaa gtatccagtc tggaaacggc atcttcatca
    13561 gtaatgaagt gcgtcatatt ggcatattcc cccaccatcg gcgtgagcgg cagttgcaaa
    13621 ccgtgaccca aatctaccac cacgccgcca tcgccaatct gcataatcaa ggtgccatta
    13681 gctgatgaaa tcaatcccaa aaacgtgcag gcaaaatcgc gaaccgctaa ttctttggct
    13741 tccgcctcgg caaaaagtcg ctgacgaatg gtcagcacaa tgtccgttgc gaggacatca
    13801 ttaagcccca attccccgcc ctgcactttt tgcgacatat aagccatcgc ttcattgacg
    13861 gcgagcatcg caccttcgcc tccctgcgag gcgctacctg cgccatcagc gacaaatacc
    13921 gacaataatg gctgctggtc atttagccag gcaacctgta tctggcaggc atcctggcaa
    13981 ggcaagtccg cactgatgtg cgacgttcca acggctgatg catagaccag tcgccagctc
    14041 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
    14101 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccctgca
    14161 gtggcaaagg ctgacgaacg ctgatttgtg ccagcgtctt catatccgcc ccctgaacgc
    14221 cgatggtaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg ttggcggcag
    14281 cctgccactc atcggttggt gcaccatcgg tgatcaggaa aatccacgga cgataatagg
    14341 aaataccatt ggcacgatat tcacgtttcc gctcctcaac catatccagg gctttggtaa
    14401 tggcagagcc cattggtgta tcgccctggg caaacaggat gggcgggaaa aaattagcgg
    14461 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
    14521 tttttaaggc cagcgaatcg gcaagcagct catcgcgaaa ggtaaccaat ccggcgttaa
    14581 gttcattaat gggtctaccg ttcatagaac cagaaacatc cagcagcaaa atgcaggggc
    14641 aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
    14701 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgactgcaa taatgaaata
    14761 agagcaaacg aagaaacttt cagaattagg cccggcggcg cagaagattt tgctattcgc
    14821 ctcgcaagaa cgtgggctta cagattgaca acaagggttg agtgacataa tttcaggagt
    14881 gagggtagag cggggtttcc cccgccctgg tagtcttagt atgctgggta gctaatgact
    14941 aagagcacca cgatgatgag tagcttcatc atgacccttt ccttatttaa ggccccttcc
    15001 tcgggagggg ctttcccgtt tcagcgtccc gctgaaatct tcggcttacc tcctttcgcc
    15061 ctgtatgcag tctatcgctg acttgcggat aaaatagttt caagtgtgat tattgcgtaa
    15121 cgactcgcaa agaatatttg ctggcaggat cgcagactgc aaagtctgcg gattgacaat
    15181 tggaccgtga aggcatactt tcaggaatga gggttgagcg gggtttcccc cgccctggta
    15241 gtcttagtaa gccgggaagc ttatgactaa gagcaccacg atgatgatcc acttcatcat
    15301 gaccctttcc ttatttatgg ccccttcctc gggaggggct ttcccgtttc agcgtcccgc
    15361 tgaaatcgtc ggcttatctc ctttcgccat gcaagcagtc tatcgctaac gcgtagataa
    15421 aatagtttca tgtgttatta ctggatgcgt gctcgcaaaa gtgcccgtca ttcagacgat
    15481 tcccgacagt gtttcataat tcctccattt ttctccctta ttggctggct acactagtat
    15541 cattccgcga aacgtttcag gaagagaaac tcttaacgat gaaaggcagt tataaatccc
    15601 gttgggtaat cgtaatcgtg gtggttatcg ccgccatcgc cgcattctgg ttctggcaag
    15661 gccgcaatga ctcccagagt gcagcccctg gggcgacgaa acaagcgcag caatcgccag
    15721 cgggtggtcg ccgtggtatg cgtgccggcc cattagctcc tgttcaggcg gcgaccgcag
    15781 tagaacaggc tgttccacgt tacctcaccg ggcttggcac cattaccgcc gctaatactg
    15841 ttacggtgcg cagccgcgtg gacggtcaac tgatggcgtt acatttccag gaaggccagc
    15901 aggtcaaagc cggcgattta ctggcagaaa ttgacccaag ccagttcaaa gttgcattag
    15961 cacaagccca gggccaactg gcaaaagata aagccacgct cgccaacgcc cgccgtgacc
    16021 tggcgcgtta tcaacaactg gcaaaaacca atctcgtatc tcgtcaggaa ctggatgccc
    16081 aacaagcgct ggtcagtgaa accgaaggca ccattaaggc tgatgaagca agcgtcgcca
    16141 gcgcgcagtt gcaactcgac tggagccgta tcaccgcacc agttgatggt cgcgttggtc
    16201 tcaagcaggt tgatgttggt aaccaaatct ccagtggcga caccaccggg attgtggtga
    16261 tcacccaaac acatcctatc gatctggtct ttactctgcc ggaaagcgat atcgccaccg
    16321 ttgtgcaggc gcaaaaagcc ggaaaaccgc tggtggtaga agcctgggat cgcaccaact
    16381 cgaagaaatt aagtgaaggc acactgttaa gccttgataa ccaaatcgat gccactaccg
    16441 gtacgattaa agtgaaagca cgctttaata atcaggatga tgcgctgttt cccaatcagt
    16501 ttgttaacgc acgcatgtta gtcgacaccg aacaaaacgc cgtggtgatc ccaaccgccg
    16561 ccctgcaaat gggcaacgaa ggccattttg tctgggtgct gaatagcgaa aacaaggtca
    16621 gcaaacatct ggtgacacca ggcattcagg acagtcagaa agtggtgatc cgcgcaggta
    16681 tttctgcggg cgaccgcgta gtgacggacg gaattgatcg cctgaccgaa ggggcgaaag
    16741 tggaagtggt ggaagcccag agcgccacta ctccggaaga aaaagccgcc agccgcgaag
    16801 acacgaaaaa aggagcacgc tcctgatgca ggtgttaccc ccgagcagca caggcggccc
    16861 gtcgcgcctg tttattatgc gtcctgtcgc caccacgctg ctgatggtgg cgatcttact
    16921 cgccgggatt atcggttatc gcgccctgcc cgtttcggcg ctgccggaag tggactatcc
    16981 aaccattcag gtggtcacgc tttatccagg tgcaagcccg gatgtcatga cctctgccgt
    17041 taccgcgccg ctcgaacgcc agttcgggca gatgtctggc ctgaaacaga tgtcgtcgca
    17101 aagttccggc ggtgcgtcag ttatcacttt acagttccag ctaacattgc cgctggatgt
    17161 cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc aacctgctac cgagcgatct
    17221 gcctaacccg ccggtttaca gcaaagtgaa cccggcagat ccaccgatca tgacgctcgc
    17281 tgtcacctca accgccatgc cgatgacgca ggtggaagat atggtggaaa cccgcgtcgc
    17341 gcagaaaatc tcgcagattt ccggcgtagg gctggtgaca ctttccggcg ggcagcgtcc
    17401 ggcggtacgc gtaaaactca acgctcaggc aattgccgct ctcggcctga ccagcgaaag
    17461 tgtgcgcacc gccattactg gcgcgaacgt taactcagcg aaagggagcc ttgacggccc
    17521 ttctcgcgcg gtcacgctgt cagcgaatga ccagatgcaa tccgccgaag agtatcgcca
    17581 gctaatcatc gcctaccaga acggcgcacc aattcgtctt ggcgatgtcg caaccgtaga
    17641 gcaaggagca gaaaacagct ggctcggcgc gtgggcgaac aaagaacagg ccattgtgat
    17701 gaatgttcag cgccagcccg gtgctaacat tatctccacc gccgacagca tccggcagat
    17761 gctaccacag ctcaccgaga gtctgccgaa atcggtgaag gtgacggtac tttctgaccg
    17821 taccaccaat atccgcgcat cggtcgacga tacccagttt gagctgatga tggctatcgc
    17881 gctggtagtc atgattatct acctgttttt gcgcaatatt ccggcgacca tcattcccgg
    17941 tgttgctgta ccgctgtcgt taattggcac ctttgcggtc atggtgtttc tcgatttttc
    18001 aatcaataac ctgacgttga tggcgttaac tatcgccacc ggattcgtgg tcgatgacgc
    18061 catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa ggcgaaaaac cgctggcagc
    18121 cgcgctcaaa ggcgcgggtg aaatcggctt taccattatc tcgctgacct tctcgctgat
    18181 tgcggtattg atccccctgc tgtttatggg cgatatcgtc gggcgactgt tccgcgaatt
    18241 tgctattacc ctggcagtcg cgattttgat ctcagcagtg gtgtcgctga ccctgacacc
    18301 gatgatgtgc gcgcggatgc tcagccagga gtcgctgcgt aaacagaacc gcttctcccg
    18361 tgcctcggag aaaatgttcg acaggataat cgccgcctat ggtcgtggac tggcgaaagt
    18421 gctgaatcat ccgtggctga ccttaagcgt agcgctcagc acgctgctgc ttagcgtgct
    18481 gctgtgggtg ttcattccga aaggtttctt cccggtacag gacaacggca taattcaggg
    18541 cactttgcag gcaccgcaat ccagctcctt tgccaatatg gcccagcggc aacgccaggt
    18601 cgcggacgtg attttgcagg atccggcagt gcaaagtttg acctcatttg ttggcgttga
    18661 tggcactaac ccgtcgctga acagtgcgcg tttacagatc aatctcaaac cactggatga
    18721 acgagatgac cgggtgcaaa aagtcatcgc ccgtctgcaa acggcggtgg ataaagtgcc
    18781 gggcgtagat ctcttcctgc aaccaacgca ggacctgact attgatactc aggtcagccg
    18841 cacccagtac cagtttacct tgcaggccac gtcactggat gcgctcagta cctgggtgcc
    18901 acagttgatg gaaaaactcc agcagctgcc acagctttct gatgtctcca gcgactggca
    18961 ggacaaaggg ctggtggcgt atgtcaatgt cgacagggac agcgccagcc gtctggggat
    19021 cagcatggcg gatgtcgata acgccctgta caacgcgttt ggtcagcgac tgatttccac
    19081 tatttatact caggctaacc agtatcgcgt ggtgctggaa cacaacaccg aaaatacccc
    19141 aggcctcgcg gcgctggata ccattcgcct gaccagcagc gacggtggcg tggtgccgtt
    19201 aagctcaatt gccaaaattg agcagcgttt tgcgccgctt tccatcaacc atctggatca
    19261 gttcccggta acgactatct cctttaacgt gccggataac tattcgctgg gcgatgcggt
    19321 gcaggcgatt atggacacag agaagacgct gaatctgccg gtggatatca ccacgcagtt
    19381 ccagggcagc accctcgcct tccagtcggc gctgggcagc actgtctggc tgattgtcgc
    19441 ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac gagagcttta ttcacccgat
    19501 caccattctc tcgacgctac ccaccgcagg ggtcggcgcg ctgctggcgt taatgattgc
    19561 cggtagcgaa ctggatgtga ttgccattat cggcattatt ttgctgatcg gtatcgtgaa
    19621 gaagaacgcc atcatgatga tcgacttcgc gctggctgct gagcgcgagc aaggcatgtc
    19681 gccgcgcgaa gctatttacc aggcctgtct gttgcgtttt cgtccgatcc tgatgaccac
    19741 tctggcggct ctgcttggtg cgctgccgct gatgttgagt accggggtcg gcgcggaact
    19801 gcgtcgtccg ttaggtatcg gcatggtcgg cggtctgatt gtcagccagg tgctgacgct
    19861 gtttaccacg ccggtgattt atctgttgtt cgaccgtctg gcattgtgga ccaaaagccg
    19921 ttttgcccgt catgaagagg aggcgtaagt gaagtttttt gccctcttca tttaccgccc
    19981 ggtggcgacg attttactgt cggtcgccat taccctgtgc ggcatactgg gtttccgtat
    20041 gctgccagtc gccccgctgc cgcaggtcga ttttccggtg atcatggtca gcgcctcgct
    20101 gcccggtgcg tcaccagaaa caatggcgtc ttccgttgcc acgccgctgg agcgctcact
    20161 tgggcgcatt gccggagtca gcgaaatgac ctccagcagt tcgctcggca gcacgcgcat
    20221 tattttgcag tttgattttg accgggatat caacggcgca gcgcgcgatg tgcaggcggc
    20281 gatcaacgcc gcgcaaagtc tgctacccag cgggatgccc agccgtccga cctatcgcaa
    20341 agcgaaccca tcggatgcgc caattatgat cctcacgctg acatccgata cttattcgca
    20401 gggtgaactg tacgatttcg cctcgacaca gctggctccg acgatttcgc aaatcgacgg
    20461 tgtcggtgat gtcgatgttg gcggcagctc gctgcccgcc gtacgcgtcg ggctgaatcc
    20521 gcaggcgctg tttaatcagg gcgtctcgct ggacgacgta cgcaccgcca tcagcaatgc
    20581 caacgtgcgt aaaccgcagg gggcgctgga agatggtact caccgctggc agatccagac
    20641 taacgatgaa ctgaaaaccg ccgctgaata tcagccgctg attattcatt acaacaacgg
    20701 cggagcggtt cgtctgggcg atgtggcgac ggtgaccgac tcagtacagg atgtgcgcaa
    20761 cgccgggatg accaacgcca aaccggctat tttgctgatg atccgcaagc tgccggaagc
    20821 caatattatc cagacggtag acagcatccg cgcccgtctg ccggaattac aatccaccat
    20881 cccggcagca attgatctgc aaattgccca agatcgctcc cctactatcc gcgcgtcgct
    20941 ggaagaggtt gagcagacgc tggtaatctc agttgcactg gtgattctgg tggtgttttt
    21001 gttcctgcgt tcgggtcgcg ccaccattat tcccgccgtt gcggtgccgg tttcgctgat
    21061 tggtacgttt gcggcgatgt acctgtgtgg tttcagtctc aataatcttt cgttaatggc
    21121 gctcactatc gccactggct ttgtggtgga tgacgccatc gtggtgctgg aaaacattgc
    21181 ccgtcatctg gaagcgggga tgaaaccgct acaggccgcg ctacaaggta ctcgcgaagt
    21241 cggctttacg gtgctgtcga tgagtctgtc actggtggcg gtgttcctgc cgctgctgtt
    21301 gatgggcgga ttgccgggtc gcctgttacg cgagtttgcc gtgacgcttt ctgtcgccat
    21361 tggtatttcg ctgctggttt ccctgacgtt aacgccgatg atgtgcggct ggatgctaaa
    21421 agccagcaag ccgcgcgagc aaaagcgact gcgtggcttt ggtcgcatgt tggtagccct
    21481 gcaacaaggc tatggcaagt cgctgaaatg ggtgctcaat catacccgtc tggtgggcgt
    21541 ggtgctgctt ggcaccattg cgctgaatat ctggctgtat atctcgatcc cgaaaacctt
    21601 cttcccggag caggacaccg gcgtactgat gggtgggata caggctgacc agagtatttc
    21661 gtttcaggcg atgcgcggta agttgcagga cttcatgaaa attatccgtg acgatccggc
    21721 ggtggataat gtcaccggct ttaccggcgg ttcacgggtg aacagtggaa tgatgtttat
    21781 cacccttaaa ccacgcggcg agcggagcga aacggcccaa cagattatcg atcgcttgcg
    21841 taaaaaactg gctaaagaac cgggagcgaa tctgttcctg atggcggtac aggatattcg
    21901 cgttggcgga cgtcaggcta acgccagtta ccagtacacg ttgttatccg acgacctggc
    21961 ggcactgcgc gaatgggagc cgaaaatccg caaaaaactg gcgacattgc cagagctggc
    22021 tgatgttaac tccgatcagg aagataatgg cgcggagatg aaccttattt acgatcgcga
    22081 caccatggcg cgattgggaa ttgacgtgca agccgccaac agcctgttaa ataacgcctt
    22141 cggtcagcgg caaatctcga ccatttacca gccgatgaac cagtacaaag tggtgatgga
    22201 agtggatccg cgctataccc aggacatcag tgcactggaa aaaatgttcg ttatcaataa
    22261 cgaaggaaaa gcgatcccgc tgtcgtattt cgctaaatgg caaccggcga acgctccact
    22321 gtcggtgaat catcagggat tatcggcggc ctcgaccatt tcgttcaacc tgccgaccgg
    22381 gaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca atgacccagc ttggtgtgcc
    22441 ttcgacggtg cgcggcagtt ttgccggaac agcgcaggtg ttccaggaga cgatgaactc
    22501 acaggtgatc ctgattatcg ccgccatcgc cacggtatat atcgtactgg gtatccttta
    22561 cgagagttat gtgcatccac tgacgattct ctccacccta ccttcggcgg gcgtcggagc
    22621 gctgttggcg ctggagctgt tcaatgcccc gttcagccta attgcgctga tagggatcat
    22681 gttattaatc ggcatcgtga agaaaaacgc cattatgatg gtcgattttg cgcttgaagc
    22741 ccaacggcac ggtaacctga cgccgcagga agctattttc caggcctgtc tgctgcgttt
    22801 tcgcccgatt atgatgacta ccctggcggc gctgtttggt gcgctgccgc tggtattgtc
    22861 gggcggtgac ggctcggagt tgcggcaacc attggggatc accattgtcg gcggtctggt
    22921 catgagccag cttctgacgc tgtataccac gccggtggtg tatctctttt tcgaccgtct
    22981 gcggatgcgt ttttcgcgta aacctaaaca agcggtaacc gagtaaatga cagatcttcc
    23041 cgacagcacc cgctggcaat tgtggattgt ggctttcggc ttctttatgc agtcgctgga
    23101 caccaccatc gttaacaccg cgcttccctc aatggcgcaa agcctcgggg aaagtccgtt
    23161 gcatatgcac atggtcattg tctcttatgt actgaccgtg gcggtgatgc tgcccgccag
    23221 cggctggctg gcggacaaag tcggcgtgcg caatattttc tttaccgcca tcgtgctgtt
    23281 tactctcggt tcactgtttt gcgcgctttc cggcacgctg aacgaactgt tgctggcacg
    23341 cgcgttacag ggcgttggcg gcgcgatgat ggtgccggtc ggcaggttga cggtgatgaa
    23401 aatcgtaccg cgcgagcaat atatggcggc gatgaccttt gtcacgctgc ccggtcaggt
    23461 cgggccgctg cttggcccgg cgctcggcgg tctgctggtg gagtacgcat cgtggcactg
    23521 gatctttttg atcaacattc cggtgggaat tattggcgcg atcgccacac tgatgttaat
    23581 gccgaactac accatgcaga cgcggcgctt tgatctctcc ggctttctgt tgctggcggt
    23641 tggcatggcg gtattaaccc tggcgctgga cggcagtaaa ggtacaggtt tatcgccgct
    23701 ggcgattgct ggcctggtcg cagttggcgt ggtagcactg gtgctttatc tgctgcacgc
    23761 ccaaaataac aaccgcgccc tgttcagtct gaaactgttc cgcactcgta ccttttcgct
    23821 gggcctggcg gggagctttg ccggacgtat tggcagtggc atgttgccct ttatgacacc
    23881 ggttttcctg caaattggcc tcggtttctc gccgtttcat gccggactga tgatgatccc
    23941 gatggtactc ggcagcatgg gaatgaagcg aattgtggta caggtagtga atcgctttgg
    24001 ttatcgtcgg gtactggtgg cgaccacgct gggcctgtcg ctggtcaccc tgttgtttat
    24061 gactaccgct ctgctgggct ggtactacgt tttgccgttc gtcctgtttt acaaggaatg
    24121 gtcaactcga cacgtttctc ctccatgaac accctgacgc tgaaagatct cccggacaac
    24181 ctggcgagca gcggcaacag tctgctgtcg atgattatgc aattgtcgat gagtattggc
    24241 gtcaccatcg ccgggctgtt gctgggactt tttggttcgc agcatgtcag cgtcgacagc
    24301 ggcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgtctat catcgccctt
    24361 ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtggc tatttcacgg
    24421 cgaaaaagga gcgcgcaatg aagttctggc gacccggtat taccggcaaa ctgtttctgg
    24481 cgattttcgc cacctgcatt gtcttattga tcacgatgca ctgggcggta cgtatcagtt
    24541 ttgagcgcgg ctttatcgat tacatcaagc atggtaatga acaacggctg caaatgctcg
    24601 gcgatgcgct tggtgagcag tacgcccagc acgggaactg gcgcttcctg cgtaataacg
    24661 atcgctttgt atttcagatc ctacgttcac tggagcatga taacaacgaa gataagcccg
    24721 gccccggtat gccgccacac ggctggcgca cacaattttg ggtggttgat caaaacaaca
    24781 aagtgctggt tggcccgcga gcaccagttc cacccgacgg cacacggcgg cccattatgg
    24841 tcaatggtgc ggaagttggc gcggtgatcg cctcccctgt tgaacgactg acccgtaata
    24901 ctgatatcaa ttttgacaga caacagcggc aaaccagttg gctgattgtg gctttatcta
    24961 ccttattagc ggcgctggcg acattcccac tggcgcgcgg tttgctggct ccggtaaaac
    25021 gactggtgga tggcacgcac aaactggcag cgggtgattt cactactcgc gtagccccca
    25081 ccagcgaaga tgagttgggc agactggcag aggatttcaa tcaactcgcc agcacactgg
    25141 agaaaaacca gcagatgcgc cgcgatttta tggccgatat ttctcatgaa ctgcgcacgc
    25201 ctttagcagt actgcgcggc gaactggaag ccattcagga tggtgtgcgt aaattcacgc
    25261 cggagacgct ggcttcttta caggcagaag taggtacact gacaaaactg gttgacgatc
    25321 tccatcaatt gtctatgtct gatgaaggcg ctctcgccta tcaaaaagca ccgatagatt
    25381 tgatcccact gctggaagtg gcgggaggcg catttcgcga acgattcgcc agccgtggcc
    25441 tgaaattgca attttccctg ccagacagta ttaccgtatt tggcgatcgc gatcgtttaa
    25501 tgcagttgtt caataactta ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc
    25561 tgaaaatctc tgccgagcag cacgacaaaa cggtgcgcct gacctttgcc gacagtgcgc
    25621 cgggtgtcag tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt
    25681 cccgcaaccg cgccagcggt ggttccgggc tggggctggc gatttgcctg aacattgttg
    25741 aagcacataa cggccgcatt attgccgccc attcgccttt tggcgggctt agcattacag
    25801 tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac caatcgacga
    25861 aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctggggcagt tgctcattga
    25921 ttatctgcgt gctgcgagct atgcgccaac gttgattagc cacggcgatc aggtactgcc
    25981 atatgtgcgc cagacaccac cggatctgat cctgttggat ctgatgctcc ctggcaccga
    26041 tggcctgacg ctgtgccggg aaattcgtcg tttttctgac attccgatcg tgatggtgac
    26101 ggcaaaaatc gaagagatcg accgcctgct ggggttggag attggcgcag atgactatat
    26161 ctgcaagccg tacagcccac gggaagtggt agcgcgcgtc aaaaccattt tgcgccgttg
    26221 caaaccgcag cgtgagttgc agcaacagga tgctgaaagc ccgttgatta tcgacgaagg
    26281 tcgttttcag gcttcctggc gcggtaaaat gcttgacctg acgcctgcag aatttcgact
    26341 gctgaaaacg ctctctcatg aaccaggaaa agtgttctcc cgcgagcagt tgctgaacca
    26401 tctttatgac gactaccgcg tggtaaccga ccgtaccatc gacagccaca ttaaaaacct
    26461 gcgccgcaag ctggaatctc tcgacgccga acagtcattt atccgcgccg tttacggcgt
    26521 cggttaccgc tgggaagccg acgcctgccg catcgtttaa catttttacc tgccggatct
    26581 ggccttcaga ccagatccgg caacattatc ccacgcatag tcagcaaact gacatgatct
    26641 ctccgccctg gcaggcacca gaaaatcgct catactttaa tcggcaaaca gtccttttag
    26701 atgctgtttt cgatacgcaa tttcaatcaa ggagtcatta tggctggttg gtttgaactc
    26761 agtaagagca gtgataatca attccggttt gtgctaaaag cgggcaatgg tgagactatc
    26821 ctcaccagcg agctgtatac ctcaaaagcc tctgcggaaa agggcatcgc gtcggtacgt
    26881 agcaacagcc cgcaagaaga acgctatgag aaaaaaacgg caagcaacgg caaattctat
    26941 ttcaatctga aagccgccaa tcatcaaatt atcggctcca gccagatgta cgccaccgcg
    27001 caatctcgtg aaaccggaat tgcctccgtt aaagcaaatg gcacaagcca gacggtgaaa
    27061 gacaatacgt aattacgatg ccgggcgtag gttgttggcg tccggcgctt taccagttac
    27121 ctccacagcg ctacaatgcc cgcccttaaa gtgggggcac tcccctgacc gcttcatcag
    27181 gtgaagcgga tctgacctgt catcagaacg agagaattat gtttaaaccg gaactccttt
    27241 ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca gatgctgttt
    27301 atgcgggcca gccgcgttac tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc
    27361 ttcaactcgg cattaacgaa gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca
    27421 ttgcaccgca caacgccaag ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa
    27481 tggggccgga tgcgctgatt atgtccgatc cagggctgat tatgctggtg cgtgagcact
    27541 tccctgaaat gccgatccat ctctcggtac aggctaacgc cgtgaactgg gcgacggtga
    27601 aattctggca gcaaatgggc ctgacccgcg tgatcctctc tcgcgaactg tcactggaag
    27661 agattgaaga gatccgcaat caggtgccgg atatggaaat cgaaatcttc gttcacggcg
    27721 cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc
    27781 cgaaccaggg cacctgcacc aacgcctgcc gctgggagta caacgtccag gaagggaaag
    27841 aagatgacgt tggcaacatc gtacacaagt acgagccgat tccggtgcaa aatgttgagc
    27901 cgacgctggg tatcggcgcg ccaaccgaca aagtgtttat gatcgaagaa gcccagcgtc
    27961 cgggcgagta tatgaccgcg tttgaagatg agcacggcac ttacatcatg aactcgaaag
    28021 atctgcgcgc catcgcccat gttgaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa
    28081 tcgaaggccg taccaaatct ttctactatt gcgcacgcac cgcgcaggtt taccgtaaag
    28141 ctatcgatga cgccgctgcg ggcaaaccgt tcgataccag cctgctggaa actctggaag
    28201 gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg tcatactcac gacgattatc
    28261 agaactacga atacggttat tcagtttctg accgccagca gtttgttggt gagtttaccg
    28321 gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa taaattctcc gttggcgaca
    28381 gccttgagct gatgacgccg caaggcaaca ttaactttac ccttgagcat atggaaaacg
    28441 ccaaaggtga agcaatgccg gtcgcaccag gcgatggtta tactgtgtgg ctcccggtgc
    28501 cgcaggatct tgagctaaat tacgcgctgc tgatgcgtaa tttctccggg gaaaccacgc
    28561 gtaaccccca cggtaagtga ttaatttcga ttatttttcc cggatggaaa attcttagaa
    28621 accgatcaca tacagctgca tttattaagg ttatcatccg tttcgctgaa aaacataacc
    28681 cataaaatgc tagctgtacc aggaaccacc tccttagcct gtgtaatctc ccttacacgg
    28741 gcttattttt tatgcataag ccctatccct ggtcaccgtc ttccattgac cacatcgata
    28801 gaatctccct taatagcacg atgcctttca cttatcgaca tcgtgctcgc acaggttccg
    28861 gttacgcaca gccagaacgc gcatgtttga cgcttaccaa aaaatgaacc taaagcattg
    28921 gaatattttt gacatcattt tctgatggct gcataaaata aaaattctgc tttagtttca
    28981 tctatctgtt tgtcattatt actcacattc aataatggtg ttgaagaata tcccatcaca
    29041 gataaaataa atatgtgcat ggtagtcttg aatctattct cactctccac atttgaatgt
    29101 cagacgagca acgccatgta atcctgcacc ttctgtcttc aggtcaacta tctgcatttt
    29161 tttgccctga gtaacacaga aatgagttgc atcatttttt actatatttt ctgcaccaga
    29221 tattctaccc ctggctaaag aagcttcggc ttcggtgtag tattggttat cgagtttacg
    29281 ctgaatatta cttttatatg caaggccaaa tttaccgata cttgtctcat cattatgcac
    29341 agcacaacca gacataagaa aaacactaat taatgatata gcagctatct ttttcatctc
    29401 accttccccc attaattacc aacgacacgc tctggtgttt aaatataata atg
//
LOCUS       DAJOTB010000050        29359 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJOTB010000050 DAJOTB010000000
VERSION     DAJOTB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.050"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            413..1552
                     /gene="wza"
                     /locus_tag="OHN28_002133"
     CDS             413..1552
                     /gene="wza"
                     /locus_tag="OHN28_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCQ2297530.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTLGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1558..2001
                     /gene="wzb"
                     /locus_tag="OHN28_002134"
     CDS             1558..2001
                     /gene="wzb"
                     /locus_tag="OHN28_002134"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCQ2297531.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYALLERSAHQWAQALNAEQV"
     gene            2004..4166
                     /gene="wzc"
                     /locus_tag="OHN28_002135"
     CDS             2004..4166
                     /gene="wzc"
                     /locus_tag="OHN28_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCQ2297532.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLTGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKVLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4295..5134
                     /gene="wcaA"
                     /locus_tag="OHN28_002136"
     CDS             4295..5134
                     /gene="wcaA"
                     /locus_tag="OHN28_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCQ2297533.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5137..5625
                     /gene="wcaB"
                     /locus_tag="OHN28_002137"
     CDS             5137..5625
                     /gene="wcaB"
                     /locus_tag="OHN28_002137"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCQ2297534.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5622..6839
                     /gene="wcaC"
                     /locus_tag="OHN28_002138"
     CDS             5622..6839
                     /gene="wcaC"
                     /locus_tag="OHN28_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCQ2297535.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6814..8034
                     /gene="wcaD"
                     /locus_tag="OHN28_002139"
     CDS             6814..8034
                     /gene="wcaD"
                     /locus_tag="OHN28_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCQ2297536.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            8115..8861
                     /gene="wcaE"
                     /locus_tag="OHN28_002140"
     CDS             8115..8861
                     /gene="wcaE"
                     /locus_tag="OHN28_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCQ2297537.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLHFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8877..9425
                     /gene="wcaF"
                     /locus_tag="OHN28_002141"
     CDS             8877..9425
                     /gene="wcaF"
                     /locus_tag="OHN28_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCQ2297538.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            9452..10573
                     /gene="gmd"
                     /locus_tag="OHN28_002142"
     CDS             9452..10573
                     /gene="gmd"
                     /locus_tag="OHN28_002142"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCQ2297539.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10576..11541
                     /gene="fcl"
                     /locus_tag="OHN28_002143"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10576..11541
                     /gene="fcl"
                     /locus_tag="OHN28_002143"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCQ2297540.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11544..12023
                     /gene="gmm"
                     /locus_tag="OHN28_002144"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11544..12023
                     /gene="gmm"
                     /locus_tag="OHN28_002144"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCQ2297541.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGSDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12020..13243
                     /gene="wcaI"
                     /locus_tag="OHN28_002145"
     CDS             12020..13243
                     /gene="wcaI"
                     /locus_tag="OHN28_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCQ2297542.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAAMVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13246..14682
                     /gene="cpsB"
                     /locus_tag="OHN28_002146"
     CDS             13246..14682
                     /gene="cpsB"
                     /locus_tag="OHN28_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCQ2297543.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14875..16245
                     /gene="cpsG"
                     /locus_tag="OHN28_002147"
                     /gene_synonym="manB"
     CDS             14875..16245
                     /gene="cpsG"
                     /locus_tag="OHN28_002147"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCQ2297544.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16300..17694
                     /gene="wcaJ"
                     /locus_tag="OHN28_002148"
     CDS             16300..17694
                     /gene="wcaJ"
                     /locus_tag="OHN28_002148"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCQ2297545.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTISVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17696..19174
                     /gene="wzxC"
                     /locus_tag="OHN28_002149"
     CDS             17696..19174
                     /gene="wzxC"
                     /locus_tag="OHN28_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCQ2297546.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISYLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLAVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19353..20633
                     /gene="wcaK"
                     /locus_tag="OHN28_002150"
     CDS             19353..20633
                     /gene="wcaK"
                     /locus_tag="OHN28_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCQ2297547.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20630..21850
                     /gene="wcaL"
                     /locus_tag="OHN28_002151"
     CDS             20630..21850
                     /gene="wcaL"
                     /locus_tag="OHN28_002151"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCQ2297548.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVAIPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRLVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDACALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21861..23255
                     /gene="wcaM"
                     /locus_tag="OHN28_002152"
     CDS             21861..23255
                     /gene="wcaM"
                     /locus_tag="OHN28_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCQ2297549.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQIVKINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCENINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGRMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23430..24323
                     /gene="galF"
                     /locus_tag="OHN28_002153"
     CDS             23430..24323
                     /gene="galF"
                     /locus_tag="OHN28_002153"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCQ2297550.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24695..25780
                     /gene="rfbB"
                     /locus_tag="OHN28_002154"
     CDS             24695..25780
                     /gene="rfbB"
                     /locus_tag="OHN28_002154"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCQ2297551.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EISDSERYSFEHADICDAEAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNSLEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            25780..26679
                     /gene="rfbD"
                     /locus_tag="OHN28_002155"
     CDS             25780..26679
                     /gene="rfbD"
                     /locus_tag="OHN28_002155"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCQ2297552.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQETDATAPLNVYGETKLAGEKALQKHCAKHIIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAVDKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26738..27616
                     /gene="rfbA"
                     /locus_tag="OHN28_002156"
     CDS             26738..27616
                     /gene="rfbA"
                     /locus_tag="OHN28_002156"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCQ2297553.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27621..28163
                     /gene="rfbC"
                     /locus_tag="OHN28_002157"
     CDS             27621..28163
                     /gene="rfbC"
                     /locus_tag="OHN28_002157"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCQ2297554.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQRIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHEKNIIWNDLLLNIKWPST
                     ALITLSDKDANGERFELSEF"
     gene            28165..>29359
                     /locus_tag="OHN28_002158"
     CDS             28165..>29359
                     /locus_tag="OHN28_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCQ2297555.1"
                     /translation="MSLLKHSIWNVAGYFIPTLIAIPAFGLIARKIGVELFGLYTLAM
                     IFIGYASIFDAGLTRAVVREIALLKNRVDDCNTIIVTSIIAVIFLGFIGGGGVFLLKG
                     DIIELLNISPIYYADSIKSLVLLSSLIPVFLVTQILLAELEGREYFGILNIQKSVGNS
                     LIAGLPALFVLINQTLFSAIIGVAIARVICLWLSYIMSRERITIDISFFSITVLKRLF
                     RYGGWVTISNIISPILASMDRFILSHIQGASKISFYTVPNELVTRLGIVPGSLGKAVF
                     PKLSHARNFTASYAEQKKAYILMTVIVMPLVLFVYYYAKFILTLWMGAEYAGISVEIL
                     RIMLIGYIFNCYSQISFANIQAFGKAKYTAYIHMMEFIPYLIMLYIISKEYGVIGVAW
                     LWTIRV"
BASE COUNT         7427 a         6949 c         7813 g         7170 t
ORIGIN      
        1 aattcttaaa aatagccaat tgccgaattg ttaccttgcc tgctattccg ttagctgtaa
       61 cacttcctcc tgcattattg gagagccaat attcaattac cataatgtca ccttatattc
      121 ggaagttaat ttccgcaata accctttctc atcaacgact gtatgcagag agggtgtgaa
      181 aaattaagct aaatcagtgt gttggtagct aaaaagccag gggcggtagc gtgtctggat
      241 gcctgaaaga ccagtctgaa ttattctgtc aatagcctgc ggatgaaatc aatttttttt
      301 aggactgatg ccagttaaat ttttattcaa ttaactgcaa ggtaaatgtg catggcgaca
      361 ttgctattaa tagtgcacag gataattact ctgccaaagt gataaataaa caatgatgaa
      421 atccaaaatg aaattgatgc cattattggt gtcagtaacc ttgataagcg gttgcacagt
      481 acttccgggc agcaatatgt cgacgctggg gaaagacgtc atcaaacagc aggacgctga
      541 tttcgatctc gacaaaatgg tgaatgttta tccgctgacc ccgcgcctga ttgatcaatt
      601 acgcccacgc ccgaatgtag cgcgccccaa tatgacgctg gaaagcgaga tcgcgaatta
      661 ccagtatcgc gtcgggccgg gggacgttct taatgtcacc gtctgggatc acccggaact
      721 caccacgcca gccggtcagt accgcagctc cagcgacacc ggcaactggg tacagcctga
      781 cggcaccatg ttctacccgt atatcggcaa agttcacgtg gtggggaaaa cgctcgctga
      841 aatccgcagt gatattaccg ggcgcttagc gacgtacatc gctgacccgc aggtggacgt
      901 taatatcgcc gccttccgct cgcaaaaagc ctatatctcc ggtcaggtga ataaatccgg
      961 tcaacaggcc attaccaacg tgccactgac cattctcgac gccatcaacg ccgcaggtgg
     1021 cctgaccgac accgctgact ggcgcaacgt ggtgctaaca cacaatggtc gtgaagagcg
     1081 catttctttg caggcgctga tgcaaaacgg cgacctcaat cagaaccgac tgctttaccc
     1141 cggcgatatt ctttacgtgc cacgtaatga tgatctgaaa gtgtttgtga tgggtgaagt
     1201 gaagaaacag agcaccctga aaatggactt tagcggcatg accctgactg aagccctggg
     1261 caatgctgaa ggcatcgaca tgaccacctc caacgccagc ggcatctttg tcattcgtcc
     1321 gctgaaaggc gagagcgggc gtaacggcaa gattgccaat atctaccagc tggatatgtc
     1381 cgatgcgacg tcgctggtaa tggcgacaga attccgcctg caaccttatg acgtggtgta
     1441 tgtcaccacc gccccggtat cccgctggaa ccgtctgatc aatcagttgc tgccaactat
     1501 tagcggtgtc cgttacatga cggatacagc cagcgacatt cataactggt aatcgtcatg
     1561 tttaacaaca tcttagttgt ctgtgtcggc aatatttgcc gttccccgac ggcggaacgc
     1621 ttactgcaac gttatcaccc ggagctaaaa gtggagtccg ctggactcgg cgcgttggtc
     1681 ggtaagggcg ctgatcctac cgctatcagc gtcgccgcag aacatcaact gtccctggaa
     1741 ggtcactgtg cacggcaaat cagccgccgc ctgtgtcgca actacgacct gattctgacc
     1801 atggaaaagc gccatatcga acgcttatgc gagatggcac ccgagatgcg cggcaaagtg
     1861 atgctgtttg gtcactggga taacgaatgt gaaatccccg atccatatcg caaaagccgg
     1921 gaaacgtttg cagcggtgta cgcattactt gaacggtctg cccaccagtg ggcgcaggca
     1981 ttgaacgcag agcaggtata agaatgacag aaaaagtaaa acaacatgcc gctccggtaa
     2041 cgggcagtga tgaaatcgat attggtcgcc tggttggcac catcattgaa gcacgctggt
     2101 gggtgattgg cattaccgcc gtattcgccc tctgtgccgt ggtttacacc ttcttcgcta
     2161 cgccgatata cagcgccgac gcactggtac aaatcgaaca aagcagcggc aattcgttag
     2221 tgcaggatat cggatcggca ttagccaaca aaccgcctgc atcggacgcc gagatccagt
     2281 tgattcgttc gcgtctggtg cttggtaaaa cggtggacga tctcgacctt gatattgcgg
     2341 tgagcaaaaa cacgttcccg attttcggtg cgggctggga gcgcctgacg ggacgccaga
     2401 acgagacggt gaaagtgacc acctttaacc gaccgaagga gatggcggat caggtgttta
     2461 cgcttaatgt gctggacaac aaaaactaca ccctgagcag cgatggcggc tttagcgccc
     2521 gtgggcaagc gggccagatg ctgaaaaaag aaggcgtcac gctgatggtt gaagccattc
     2581 acgccagccc gggcagtgag tttaccgtca ccaaatactc cacgctgggg atgatcaacc
     2641 aactgcaaaa cagcctgacg gtaacggaga acggcaaaga cgcaggtgtt ctgagcctga
     2701 cttacaccgg tgaagatcgc gaacagatcc gcgacattct taacagcatc gcccgtaact
     2761 atcaggaaca aaatattgag cgcaaatcgg cggaagcgtc gaaaagcctc gctttcctcg
     2821 cgcaacagtt accggaagta cgtagccgcc ttgatgttgc cgaaaacaaa ctgaatgcct
     2881 tccgtcagga taaagattca gttgatctgc cgctggaagc gaaagcggtg ctcgattcga
     2941 tggtgaacat cgacgcgcag ttgaacgaac tgacctttaa agaggcggaa atctccaagc
     3001 tgtacaccaa agttcacccg gcgtaccgca cgctgctgga gaaacggcag gcgctggaag
     3061 acgaaaaagc caaacttaat ggtcgcgtaa cggcgatgcc gaaaacccag caggaaattg
     3121 tgcgtctgac ccgcgatgtc gagtctggtc agcaggtcta tatgcaactg ctgaataaag
     3181 agcaggagct gaaaatcacc gaagccagca ccgtcggcga tgtgcgcatt gtcgacccgg
     3241 caatcaccca gccaggtgta ctgaaaccga agaaagtgct gattatcctt ggggcgatta
     3301 tccttggcct gatgctctct atcgtgggtg tgctgctgcg ctcgttgttt aatcgcggta
     3361 tcgaaagccc gcaggtgctg gaagaacacg gtatcagcgt ttatgccagc atcccgctgt
     3421 cggagtggca gaaagcgcgc gatagcgtca aaaccatcaa agggattaaa cgctataaac
     3481 agagccagct actggcggtg gggaatccaa ccgatctggc gattgaagcc atccgtagtc
     3541 tgcgtaccag tctgcacttc gcgatgatgc aggcacagaa caatgtgttg atgatgaccg
     3601 gggttagccc gtcaatcggt aaaacctttg tctgcgccaa cctggcggcg gtgattagcc
     3661 agaccaataa acgggtgttg ttgatcgact gcgatatgcg caaaggctac acccacgagc
     3721 tgttgggcac taataacgtt aatggcctgt cggaaattct gattggacag ggcgatatta
     3781 ctaccgctgc taaaccgacc tctattgcca aatttgacct gatcccgcgc ggtcaggtac
     3841 cgccaaatcc ttctgaactg ttaatgagcg aacgctttgc cgaactggtg aagtgggcga
     3901 gtaaaaacta cgacctggtg ttgattgata cgccgccgat tctggcagtg accgatgcgg
     3961 caattgttgg tcgtcatgtc ggaaccacgt tgatggtggc gcgttatgcg gtcaacacat
     4021 tgaaagaagt ggaaaccagt ctgagccgct ttgagcaaaa cggtattccg gtgaaagggg
     4081 tgattctgaa ctccatcttc cgccgcgcca gcgcgtatca ggattatggc tattacgaat
     4141 acgaatataa gtcggatgcg aaataacgag atccgctgtc ggatgcgatg ctgacgcatc
     4201 ttatccgacc tacgttcacg ccgcatccgg caacagaacc tgtaaaccag cttataaacc
     4261 ggcagaacat ctcgatactc cgggagaaca acctatgaaa gacaatccgc tgatttcaat
     4321 ctatatgccg acctggaacc gccaacaact ggcgattcgg gcgataaaat cggtcctgcg
     4381 ccaggactac aacaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca
     4441 actacaacag tacgtcaccg ccctcaacga tccgcgtatc acttacattc acaacgacat
     4501 taacagcggg gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatacat
     4561 caccgggatc gatgacgatg atgaatggac acccaaccgc ctgagcgtct tcctcgccca
     4621 taaacagcaa ctggtcacac acgccttttt gtacgctaac gactacgttt gccagggcga
     4681 agtttattcc caaccggcaa gcttgccgct gtatcccaaa tcgccgtatt cacgccgcct
     4741 gttttacaaa cgcaatatca ttggcaatca ggtctttacc tgggcgtggc gtttcaaaga
     4801 gtgcttgttc gataccgaac tgaaagcggc acaggattac gacatcttcc tgcggatggt
     4861 ggtggagtac ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca
     4921 cggcgaaatg cagatcacct cgtcaccgaa aaaattctcc ggatacttcc acttttaccg
     4981 caagcacaaa gacaaattcg accgcgccag caaaaaatat cagctgttta ccctctacca
     5041 gatccgcaat aaacgcatga cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg
     5101 caaacgtctg gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgcgccaac
     5161 agctggagtt tacgcccgtg ctgcatggtt cttgcctatc gtgccgctca tttttgttcg
     5221 gtgtggcgca aaaagaacgt cctcaacaat ctttgggcgg ccccgctgct ggtgctgtat
     5281 cgcattatca ccgaatgctt tttcggttat gaaatccagg ccgccgcgac cataggccga
     5341 cgctttacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt cgcgggggat
     5401 gatttcacca ttcgtcacgg cgtcactatc ggcaatcgtg gtgccgataa catggcatgt
     5461 ccacacattg gcaacggcgt cgaactcggt gccaacgtca ttattcttgg tgatatcacg
     5521 attggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac
     5581 gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ctgcaattta
     5641 atgtgcgact ggcggaaggc ggggcagcag gtgtggcatt agatctccac cagcgcgcgc
     5701 tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc aaagagagcg
     5761 tcagccatca gaactatccg caggtcatca aacatacgcc acggatgacc gcgatggcga
     5821 acattgcgtt gtttcgtctg cttaatcgcg atctgttcgg caatttcaat gagttatatc
     5881 gcaccattac tcgcacaccg ggcccggtgg ttctgcattt tcatgtgctg cacagctact
     5941 ggttgaatct taagagcgtg gtgcgctttt gcgaaaaagt gaaaaaccat aaaccggatg
     6001 tcactctggt ctggacgcta cacgaccact ggagcgttac cggacgctgc gcctttaccg
     6061 acggttgcga aggctggaaa acgggctgcc agaaatgccc gaccttaaat aactatccac
     6121 cggtgaagat tgatcgcgcg caccagctgg tggcgggtaa acgccagtta ttccgcgaga
     6181 tgctggcact gggctgtcag tttatttccc ccagccagca tgtggctgat gctttcaata
     6241 gtctgtacgg tccagggcgt tgccggatta tcaataatgg cattgatatg gcaaccgaag
     6301 cgattctggc ggacttgcct ccggtacgcg aaacccaggg caagccgaaa atcgcggtgg
     6361 tggcgcatga cctgcgttac gacggcaaaa ctaaccagca actggtacgc gagatgatgg
     6421 cgctgggtga caaaatcgaa ctgcatacct ttggtaagtt ctcgccgttc accgctggta
     6481 acgttgtcaa tcacggcttt gaaactgaca agcgcaagtt aatgagcgtg ctcaatcaga
     6541 tggatgcgct ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgt
     6601 tatcgattgg cgtgccggtg atcgccaccc atagcgatgc ggcgcgggaa gtgctgcaaa
     6661 aatccggtgg taaaaccgtc agcgaagaag atgtgctgca actggtgcag ttaagcaaac
     6721 cggaaatcgc acaggcgata tttggtacca cgctggctga attcagccag cgcagccgcg
     6781 ccgcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc
     6841 tacctgctgc tgccgctgat ctacctgctg gttaacgtca aaatcgccca gcttggcgaa
     6901 agtttcccca tcaccatcgt cactttttta cctgtcctgt tactgctgtt tttagagcgc
     6961 atcagcgtta aaaaattgat gattgccttg gggattggcg cgggacttac ggcgtttaac
     7021 tacctgtttg gtcagtcgct ggatgccagt aaatacgtca cttcaaccat gctgtttgtc
     7081 tatattgtga tcattattgg catggtgtgg agtattcgct ttaaaacgat ttcgccacac
     7141 aaccatcgca agatattacg tttcttttat ctggtggtcg ggctggtggt ggtgctggcg
     7201 gcggtggaga tggcgcaaat tatcctcacc ggcggtagca gtattatgga gtcgatttcg
     7261 aaatatctga tttacagcaa cagctatgtg ctgaatttca ttaaattcgg cggcaagcgc
     7321 acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc
     7381 agcatcaaac agtttggtat caaaacgcct aaaacagatg ctatgattct cgcagggata
     7441 atattatccg gttcattttc aggggttatg acctttatcc ttttttattt gctggagtgg
     7501 gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc attgatttct
     7561 ctggctgtat tcctggttgg tgtggtaatc gcgttccctt atatttccac ccgtctgggc
     7621 gatttaggta cagaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc
     7681 ggttattctt taacccatat tgacggtgta gtcagatttg gctcacttta tgaatatgtc
     7741 gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat
     7801 ctgctgatta tttatttttc ctggttcgca gtgtttttat cgctgtggta catgggaaaa
     7861 gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat
     7921 ctttttactc cggtatcgct gttttttacc ggttcgatat ttagcccaga atatgcgttt
     7981 ttaatcgtct gcccgtttat tttgcgaaaa gcgttaaata tacaccctaa ataattcgag
     8041 ttgcaggaag tgggtgagcg aggaaaacca acgcacatgc agcttgaagt atgacgggta
     8101 ttacgaggta aaacatgttg cttagcataa tcactgtcgc gtttcgtaac ctcgaaggaa
     8161 tagtcaaaac acacgcctcg ctggcgcatc tggcgcaggc ggacgatatc agcttcgaat
     8221 ggattgttgt tgatggcggt tccaacgacg gcacccgtga gtatctggaa aatctcaatg
     8281 gtatctataa cttacacttt gtcagcgaac cagataacgg tatctacgac gccatgaaca
     8341 aaggtattga aatggcgcaa ggcaagttcg cgttgtttct caactcgggc gatatttttc
     8401 atcaggatgc cgcatatttt gtccgtaagt taaaagtgca aaaagataac gtgatgatca
     8461 ccggcgatgc gctgctggat tttggcgacg ggcataaaat taaacgtagc gccaaaccgg
     8521 gttggtatat ttaccacagc ctgcccgcca gtcatcaggc gatatttttc ccggtgtccg
     8581 gcttgaagaa atggcgttat gacctggagt ataaagtttc ttccgattac gcgctggcgg
     8641 ccaaaatgta taaagccggt tatgcattta aaaaactcaa tggcctggtg tctgaatttt
     8701 ctatgggtgg ggtatctacc accaataata tggaattgtg tgctgacgcc aaaaaagtcc
     8761 aacggcaaat attgcacgtg cctggctttt gggctgaatt atcctggcat ttacgccaac
     8821 gtacgacctc aaagacgaaa gccttatata acaaaagctg aatataaggg aaaacgatgc
     8881 aggatttaag tggattctcg gtgccgaaag ggttccgggg tggcaacgct attaaagtgc
     8941 aattgtggtg ggcagtacag gcaacaatat ttgcctggtc gccacaagta ttgtatcgct
     9001 ggcgggcatt tttattacgt ttattcggcg caaaaatagg aaaaaacgta gtcattcgtc
     9061 cgtcagtaaa aattacctat ccgtggaaat taacgttagg tgattacgcg tgggtcggcg
     9121 atgaggtcaa tttatatacc ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc
     9181 aaaaaagtta tttatgcact ggtagccacg accatgcaag tcaacatttc accattaacg
     9241 ccacgcctat tgtgattggc gagaaatgct ggctggcaac cgatgtcttt gttgcccctg
     9301 gcgtcacgat cggcgacggc accgtcgtgg gtgcgcgaag cagtgttttt aaatcgcttc
     9361 cggcaaatgt ggtttgtcgg gggcaccccg cagtggtgat acgcaaacgc gttgaaactg
     9421 aataaattca aaaaatacag aggaataaga catgtcaaaa gtcgctctca tcaccggtgt
     9481 aaccggacaa gacggttctt acctggcaga gtttctgctg gaaaaaggtt acgaggtgca
     9541 tggtattaag cgtcgtgcat cgtcattcaa caccgagcgc gtggatcaca tttatcagga
     9601 tccgcacacc tgcaacccga aattccatct gcattatggc gacctgagtg atacctccaa
     9661 cctgacgcgc attttgcgtg aagtgcagcc ggatgaagtg tacaacctgg gggcaatgag
     9721 ccacgttgcg gtttcttttg agtcaccgga atataccgca gacgtcgatg cgatgggtac
     9781 gctgcgcctg ctcgaggcga tccgcttcct cggtctggaa aagaaaaccc gtttctatca
     9841 ggcttctact tctgaactgt acggtctggt gcaggaaatt ccgcagaaag agaccacgcc
     9901 gttctacccg cgatctccgt atgcggttgc caaactgtac gcctactgga tcaccgttaa
     9961 ctaccgcgaa tcctacggca tgtacgcctg taacggtatt ctcttcaacc atgaatcccc
    10021 gcgccgtggc gagactttcg ttacccgcaa aatcacccgc gcaatcgcca acatcgccca
    10081 ggggctggag tcgtgcctgt atctcggcaa tatggattcc ctgcgtgact ggggccacgc
    10141 caaagactac gtaaaaatgc agtggatgat gctgcaacag gaacaaccgg aagatttcgt
    10201 tattgccacc ggcgttcagt actccgtgcg tcagttcgtg gaaatggcgg cggcacaact
    10261 gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag aagggcattg tggtttccgt
    10321 caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg attatcgccg ttgacccgcg
    10381 ctacttccgt ccggcagaag ttgaaacgct gctcggtgac ccgaccaaag cgcacgaaaa
    10441 actgggctgg aaaccggaaa tcaccctcag agagatggtg tctgaaatgg tggctaatga
    10501 ccttgaagcg gcgaaaaaac actctctgct gaaatctcac ggctacgacg tggcgatcgc
    10561 gctggagtca taagcatgag taaacaacgc atttttattg ctggccatcg cgggatggtc
    10621 ggttccgcca tcaggcggca gctcgaacag cgcggtgatg tggagctggt attacgcacc
    10681 cgcgatgaac tgaacctgct ggacagccgc gcggtgcatg atttctttgc cagcgaaagc
    10741 attgaccagg tctatctggc ggcggcgaaa gtgggtggta ttgttgccaa caacacgtat
    10801 ccggcagatt tcatctacca gaacatgatg attgagagca acatcattca cgccgcgcat
    10861 cagaacgacg tgaacaaact gctgtttctc ggatcgtcct gcatctaccc gaaactggca
    10921 aaacagccga tggcagaaag cgagttgttg cagggcacgc tggagccgac caacgagcct
    10981 tacgccattg ccaaaatcgc cgggatcaaa ctgtgtgaat cttacaatcg ccagtacgga
    11041 cgggattacc gctcagtcat gccaaccaac ctgtacgggc cacacgacaa cttccacccg
    11101 agtaattcgc atgtgatccc agcattgctg cgtcgcttcc atgaggcgac ggcacagaat
    11161 gcgccggacg tggtggtgtg gggcagcggt acgccgatgc gcgaatttct acacgttgat
    11221 gatatggcag cggcgagcat tcatgtcatg gagctggcac acgaagtctg gcaggagaac
    11281 acccagccga tgctgtcgca cattaacgtc ggcacgggcg ttgactgcac tatccgtgag
    11341 ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc gggtggtttt tgatgccagc
    11401 aaaccggatg gcacgccgcg caaactgctg gatgtgacgc ggctgcatca gcttggctgg
    11461 tatcacgaaa tctcactgga agcggggctt gccagcactt accagtggtt ccttgagaat
    11521 caagaccgct ttcgggggta atgatgtttt tacgtcagga agactttgcc acggtagtgc
    11581 gctccactcc gcttgtctct ctcgacttta ttgtcgagaa cagtcgcggc gagtttctgc
    11641 ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt tgtgccggga gggcgcgtgc
    11701 agaaagacga aacgctggaa gccgcatttg agcggctgac gatggcggaa ctggggttgc
    11761 gtctgccgat aacagcaggc cagttttacg gtgtctggca gcacttttat gacgataact
    11821 tctccggctc ggattttacc actcactatg tggtgctcgg ttttcgcttc agagtagcgg
    11881 aggaagagct gttactgccg gatgagcagc atgacgatta ccgctggctg acgccggacg
    11941 cgttgctcgc cagtgacaat gttcacgcta acagccgcgc ctattttctc gctgagaagc
    12001 gtgccggagt acccggatta tgaaaatact ggtctacggc attaactact cgccggagtt
    12061 aaccggcatc ggcaaataca ccggcgagat ggtggaatgg ctggcggcac aaggtcatga
    12121 ggtgcgggtc attaccgcgc cgccttacta cccgcagtgg caggtgggcg agaactattc
    12181 cgcctggcgg tacaaacggg aagagggggc cgccatggtg tggcgctgcc cgctgtacgt
    12241 gccaaaacag ccgagcactc tgaaacgcct gttgcatctc ggcagttttg ccgtcagcag
    12301 tttctttcca ctgatggcgc aacgtcgctg gaagccggat cgcattatcg gcgtagtgcc
    12361 aacgctgttt tgcacgccgg gaatgcgcct gctggcgaaa ctctctggtg cgcgtaccgt
    12421 gctgcatatt caggattacg aagtggacgc catgctgggg ctgggccttg ccggaaaagg
    12481 caaaggcggc aaagtggcac agctggcgac ggcgttcgaa cgtagcggac tgcataacgt
    12541 cgataacgtc tccacgattt cgcgttcgat gatgaataaa gccatcgaaa aaggcgtggc
    12601 ggcggaaaac gtcattttct tcccaaactg gtcggaaatc gcccgttttc agcatgtcgc
    12661 agacgccgat gttgatgccc ttcgtaacca gcttggcctg ccggataaca aaaaaatcat
    12721 tctttactcc ggcaatattg gtgaaaagca ggggctggaa aacgttattg aagctgccga
    12781 tcgcctgcgc gatgaaccgc ttatttttgc cattgtcggg cagggcggcg gcaaagcgcg
    12841 gctggaaaaa atggcgcagc agcgtggact gcgcaacatg caattttttc cgctgcaatc
    12901 gtatgacgct ttacccgcac tgctgaagat gggggattgc catctggtgg tgcaaaaacg
    12961 cggcgcggca gatgccgtat tgccgtcgaa actgaccaat attctggcgg taggcggtaa
    13021 cgcggtgatt accgcagaag cccacacaga actgggacag ctttgcgaaa cctttccggg
    13081 cattgcggtt tgcgtggaac cggaatcggt cgaggcgctg gtggcgggga ttcgtcaggc
    13141 gctcctgctg cccaaacaca acacggtggc acgtgaatat gccgaacgca cgctcgataa
    13201 agagaacgtg ttacgtcaat ttataaatga tattcgggga taattatggc gcagtcgaaa
    13261 ctctatccag ttgtgatggc aggtggctcc ggtagccgct tatggccgct ttcccgcgta
    13321 ctttacccca agcagttttt atgcctgaaa ggcgatctca ccatgctgca aaccaccatc
    13381 tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga tttgcaatga gcagcaccgc
    13441 tttattgtcg cggaacagct gcgtcaactg aataaactca ccgagaacat tattctcgaa
    13501 ccggcagggc gtaacactgc accggccatt gcgctggcgg cgctggcggc aaaacgtcat
    13561 agcccggaaa acgacccatt aatgctggtg ctggcagcgg atcacgtgat tgccgatgaa
    13621 gacgcgttcc gtgccgccgt gcgtaatgcc atgccgtatg ccgaagcggg taagctggtc
    13681 accttcggca ttgtgccgga tctacctgaa accggatatg gctatattcg tcgcggtgaa
    13741 gtgtcggcgg gcgagcagga tacggtggcc tttgaagtgg cgcagtttgt cgaaaaaccg
    13801 aatctggaaa ccgctcaggc ctatgtggca agcggcgaat attactggaa cagcggtatg
    13861 ttcctgttcc gtgccggacg ctatctcgaa gaactgaaaa aatatcgccc ggatattctc
    13921 gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc tcgattttat tcgtgtggat
    13981 gaagaagcgt ttctggcctg cccggaagag tcggtggatt acgcggtcat ggaacgaacg
    14041 gcagatgccg ttgtggtgcc gatggatgca ggctggagcg atgtcggctc ctggtcttca
    14101 ttatgggaga tcagcgccca caccgccgag ggcaacgttt gccatggcga tgtgattaat
    14161 cacaaaactg aaaacagcta tgtgtacgcc gaatctggcc tggtcaccac cgtcggggtg
    14221 aaagatttgg tggtagtgca gaccaaagat gcggtgctga ttgccgaccg taatgcggtg
    14281 caggatgtga aaaaagtggt cgagcagatc aaagccgatg gtcgccatga gcatcgggtg
    14341 catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta tcgacgcggg cgaccgctac
    14401 caggtgaaac gcatcaccgt gaaaccgggc gaaggcttgt cggtacagat gcaccatcac
    14461 cgcgcggaac actgggtggt tgtcgcggga acggcaaaag tcactatcga cggtgatatc
    14521 aaactgcttg gtgaaaacga gtccatttat attccgctgg gggcgacgca ttgcctggaa
    14581 aacccgggga aaattccgct cgatttaatt gaagttcgct ccggctctta tctcgaagag
    14641 gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt aaacgtcgca tcaggcgctg
    14701 tttgtcggat gcggcgtgaa cgccttatcc gacctacggt tcgggtttgt aggcctgata
    14761 agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg cgatgtaaat aacgacaaaa
    14821 acaaaattgg ccgtttcggt cagggccaac tattgcctga aaaagggtaa cgatatgaaa
    14881 aaattaacct gctttaaagc ctatgatatt cgcggaaaat taggcgaaga actgaatgaa
    14941 gatattgcct ggcgcattgg tcgcgcttat ggcgaatttc tcaaaccgaa aaccattgtg
    15001 ttaggcggtg atgtccgcct caccagcgaa accttaaaac tggcgctggc gaaaggttta
    15061 caggatgcgg gcgtcgacgt gctggatatt ggaatgtccg gcaccgaaga gatctatttc
    15121 gccacgttcc atctcggtgt ggatggtggc atcgaagtta ccgccagcca taacccgatg
    15181 gattacaacg gcatgaaact ggtgcgcgaa ggggctcgtc cgatcagcgg agataccgga
    15241 ctgcgcgacg tccagcgtct ggcagaagcc aacgactttc ctcccgtcga tgaaaccaaa
    15301 cgcggtcgct atcagcaaat caatctgcgt gacgcttacg ttgatcacct gttcggttat
    15361 atcaatgtca aaaaccttac gccgctcaag ctggtgatca actccgggaa cggcgcagcg
    15421 gggccggtgg tggacgccat tgaagcccgc tttaaagccc tcggcgcacc ggtggaattg
    15481 atcaaagtgc ataacacgcc ggacggcaat ttccccaacg gtattcctaa cccgctactg
    15541 ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac acggcgcgga tatgggcatt
    15601 gcctttgatg gcgattttga ccgctgtttc ctgtttgacg aaaaagggca gtttattgag
    15661 ggctactaca ttgtcggcct gttggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag
    15721 atcatccacg atccgcgtct ctcatggaac accgttgatg tggtgacggc cgcaggcggc
    15781 accccggtaa tgtcgaaaac cggacacgcc tttattaaag aacgtatgcg caaggaagac
    15841 gctatctacg gtggcgaaat gagcgcccac cattacttcc gtgatttcgc ttactgcgac
    15901 agcggcatga tcccgtggct gctggtcgcc gaactggtgt gcctgaaagg aaaaacgctg
    15961 ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa gcggtgagat caacagcaaa
    16021 ctggcacaac ctgttgaggc gattaaccgc gtggaacagc actttagccg cgaggcgctg
    16081 gcggtggatc gtaccgatgg catcagcatg acctttgccg actggcgctt taatctgcgc
    16141 tcctccaaca ccgaaccggt ggtgcggttg aatgtggaat cacgcggtga tgtgccgctg
    16201 atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg agtaatgtcg gatcttccct
    16261 taccccactg cgggtaaggg gctaaaaaca ggaacaacga tgacaaatct aaaaaagcgc
    16321 gagcgagcga aaaccaatgc atcgttaatc tctatggtgc aacgcttttc agatatcacc
    16381 atcatgtttg ccgggctatg gctggtttgc gaagtcagcg gactgtcatt cctctacatg
    16441 cacctgttgg tggcgctgat tacgctggtg gtgttccaga tgctgggcgg catcaccgat
    16501 ttttatcgct catggcgcgg tgttcgggca gcgacagaat ttgccctgtt gctacaaaac
    16561 tggaccataa gcgtgatttt cagcgccgga ctggtagcgt tcaacaatga tttcgacacg
    16621 caactgaaaa tctggctggc gtggtatggg ctgaccagca tcggactggt ggtttgccgt
    16681 tcatgtattc gtattggggc gggctggctg cgtaatcatg gctataacaa gcgtatggtc
    16741 gccgtagcgg gggatttagc cgccggacaa atgctgatgg agagcttccg taatcagccg
    16801 tggttagggt ttgaagtggt gggcgtttac cacgacccga aaccgggtgg cgtttctaac
    16861 gactgggcgg gcaatctgca acaactggtc gaggatgcga aagcgggcaa gattcataac
    16921 gtctatatcg cgatgcagat gtgcgacggc gcgcgagtga aaaaactggt ccatcaactg
    16981 gcggacacca cctgttcggt gctgctgatc cccgatgtct ttaccttcaa cattctccat
    17041 tcacgcctcg aagagatgaa tggcgttccg gtggtgccgc tgtacgacac gccgctttcc
    17101 ggggttaacc gcctgataaa acgtgcagaa gacattgtgc tggcgacgct tattctgctg
    17161 ctgatctccc cggtgctgtg ctgcattgcg ctggcggtga aactgagctc accggggccg
    17221 gttattttcc gccagactcg ctacggcatg gatggcaagc cgatcaaagt gtggaagttc
    17281 cgttccatga aagtgatgga gaacgacaaa gtggtgaccc aggcgacgca gaacgatccg
    17341 cgcgtcacca aagtggggaa ttttctgcgc cgcacctcgc tggatgaatt gccgcagttt
    17401 atcaatgtgc tgaccggggg gatgtcgatt gtgggtcccc gtccgcatgc ggtggcgcat
    17461 aacgaacagt atcgccagct cattgaaggc tacatgctgc gccataaagt gaaaccgggc
    17521 attaccggct gggcgcagat taacggctgg cgcggcgaaa ccgacacgct ggagaaaatg
    17581 gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat ggagcgtctg gttcgatatc
    17641 aaaatcgttt tcctgacggt attcaaaggt ttcgttaaca aagcggcata ttgatatgag
    17701 cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggcg attgccacgg tgatcatcat
    17761 cggcctcgga ctggtgcaaa tgactgtgct ggcgcggatt atagataacc accagttcgg
    17821 tttgctgact gtgtcgctgg tgatcatcgc gctggcagat acgctttctg acttcggtat
    17881 cgctaactcc attattcagc gaaaagaaat cagctacctt gaactcacca cgctgtactg
    17941 gctgaacgtt gggctggggc ttgtggtgtg cgtggcggtg tttttgttga gtgatctcat
    18001 cggtgacgtg ttgaataacc cggacctggc accgttgatt aaaacattgt cgctggcgtt
    18061 tgtggttatc ccccacgggc aacagttccg cgcgttgatg caaaaggagc tggagttcaa
    18121 caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc ttcactttta cagtggttag
    18181 cgcccatttc tggccgctgg cgatgaccgc gattctcggt tatctggtca atagtgcggt
    18241 aagaacgctg ctgtttggct actttggtcg aaaaatttat cgccccggtc ttcatttctc
    18301 gctggcgtcg gtggcaccaa acttacgttt tggtgcctgg ctgacggcgg acagcatcat
    18361 caactatctc aacaccaacc tttcaacgtt ggtgctggcg cgtattctcg gcgcgggcgt
    18421 agcaggggga tacaacctgg cgtacaacgt ggccgttgtg ccgccgatga agctgaaccc
    18481 aatcatcacc cgcgtgttgt ttccggcatt tgccaaaatt caggacgaca ccgaaaagct
    18541 gcgcgttaac ttctacaagc tgctgtcggt agtggggatt atcaactttc cggcactgct
    18601 ggggctgatg gtggtgtcga ataactttgt accgctggtc tttggtgaga agtggaacag
    18661 cattattccg gtgctgcaat tgctgtgtgt ggtggggctg ctgcgctcag tggggaaccc
    18721 gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc agctttaaat tcaacgtatt
    18781 caaaaccttt ctgtttattc cggcgattgt tattggtggg cagatggcgg gcgcaatcgg
    18841 cgtcacgctt ggcttcctgc tggtgcagat tatcaacacc attctgagct atttcgtgat
    18901 gattaaaccg gtgctcggtt ccagttatcg ccagtacatc ctgagtttat ggctgccgtt
    18961 ttatctctcg ctgccgacgc tggcggtcag ttatgcgctg ggcattgtgc tgaaagggca
    19021 actggcgctg gggatgctgc tggcggtgca aatagccgca ggcgtgctgg cgtttgtggt
    19081 gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg aaacgtcagt tttgtcgcag
    19141 cgaaaaaatg aaaatgcttt tacgggcggg gtgagttgtt gttttcggtt ccgacaacgg
    19201 tttccggatg cggcgtgaac gccttatccg tcctacggtt ctgtgctcgg gacttgtagg
    19261 tctgataaga cgcgttagcg tcgcatcagg caaaaaacag caatgaaaac aacaaacata
    19321 gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca
    19381 cttgcggcaa tcgtggcgac agcgccatcc tgcgcggctt acttgatgcc atcaatatcc
    19441 tcaatccaca tgccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc
    19501 tcaaccgccc ggtaatgggc gatccgctgt tcctgcaaat gaaacaacac aacagcgcgg
    19561 cgggcgttgt cgggcgcgtt aaaaaagtcc tccgtcgccg ctaccagcac caggtattgc
    19621 tctcacgcgt caccgacact ggcaagctgc gtaatatcgc catcgcccag ggattcaccg
    19681 acttcgtgcg cctgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg
    19741 tcgatctcta cggcgttccg cagtttgaac atgcgctttg cacgtttatg gcgaaaaagc
    19801 cgctgtttat gattggtcac agcgtcggcc cgttccagga tgagcaattt aaccaactgg
    19861 cgaactacgt attcggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgaac
    19921 tgatgaaacg cagcaatatc accaccgcaa aagtcgaaca tggcgtcgat accgcgtggc
    19981 tggtcgatca ccacaccgaa gacttcaccg ccagctatgc cgttcaacac tggctggacg
    20041 ttgccgcaca acagaaaacg gtggccatta ccctgcgcga actggcaccg tttgacaaac
    20101 gtctcggcac cacccaacaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc
    20161 tcgatgaagg gtatcaggtg attgcgctct ccacctgtac gggcatcgac agctataaca
    20221 aagacgaccg catggtggcg ctcaacctgc gccagcatat cagcgatcct gcccgttacc
    20281 acgtagtgat ggatgaactt aacgatctgg aaatgggcaa aattctcggt gcctgtgaac
    20341 tcaccgtcgg cacgcgcctg cactctgcca ttatctcgat gaactttgcc actccggcga
    20401 ttgccatcaa ctatgaacat aaatccgctg ggattatgca acaactgggg ctaccagaga
    20461 tggcaattga tatccgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct
    20521 taggtcaact tccggcgctg aatgcacgac ttaacgaagc cgtcagtcgc gaacgtcaga
    20581 cgggaatgca gatggtgcag tctgtgcttg agcgcatcgg ggaagtgaaa tgaaggttgg
    20641 cttcttttta ctgaaattcc cgctgtcgtc agaaaccttc gtccttaacc aaattaccgc
    20701 gtttattgat atgggatttg aggtggagat tctcgcgctg caaaaaggcg acacccagaa
    20761 cacccacgcg gcatggacga aatacaacct tgccgccaga acccgctggt tacaggacga
    20821 acctacgggc aaagtggcga agctgcgcca ccgcgccagc cagaccttac gcggcattca
    20881 tcgtaaaaat acctggcagg cgctcaacct caaacgctat ggtgccgagt cgcggaacct
    20941 gattttgtct gccatttgtg gtcaggtcgc aataccattt tatgccgatg tctttatcgc
    21001 tcattttggc ccggcggggg taaccgcagc aaaactacgc gaactgggtg tcattcgcgg
    21061 caaaattgcc actatcttcc acggcattga tatttccagt cgggaagtgc tcaaccatta
    21121 cactcccgaa tatcaacaac tgtttcgccg tggcgacctg atgctaccaa taagcgatct
    21181 gtgggccgga aggctgcaaa aaatgggctg cccgagggaa aaaatcgccg tatcgcgcat
    21241 gggcgtggac atgacgcgct ttagcccgcg tctggtgaaa gcacctgcaa cgccgctgga
    21301 aatcatttcc gtcgcacgct taaccgagaa aaaaggcctg catgtggcga ttgaagcctg
    21361 ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg
    21421 ggaacgacgc ctgcgcaccc tcatcgaaca atatcaactg gaagatgtga tagagatgcc
    21481 gggctttaaa ccgagccatg aagtgaaagc gatgctcgat gacgcggatg tcttcctgtt
    21541 gccatcggtt acaggtgcgg atggcgatat ggaaggtatt ccggtggcgc taatggaagc
    21601 gatggcggtt ggcattccgg ttgtttctac tctgcacagc ggaataccgg aactggtgga
    21661 ggccgataaa tccggctggc tggtgcctga gaacgatgcc tgtgcactgg cgcaacgact
    21721 ggcggcgttt agccaactgg acaccgacga acttactacg gttgtcaaac gtgcgcgcga
    21781 aaaagtcgaa cacgatttta accagcaggt gattaatcga gaactcgcca gcttgctgca
    21841 ggctttatag aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg
    21901 caagctcggc gcttgccttc ctccataccc ctttcgctcg cgcgcttccc gcccgacaaa
    21961 tcgttaagat taacgactac aacccacacg actggatcgc ctcatttaaa caagccttca
    22021 gcgaagggca aacggtcgtc gtgcctgccg gattcgtttg tgaaaatatc aacaccggca
    22081 tcttcattcc tcctggcaaa acgttacaca tccttggaag cctgcgcggc aacggcagag
    22141 ggcgttttgt cttacaagac ggcagccagg tgacagggga gaagggcggc cgtatgcata
    22201 acatcacctt ggatgtgcgc ggttctgact gcatcatcaa agggttggcg atgagcggtt
    22261 ttggtccggt aacgcagatt tatatcggcg gcaaaaataa acgcgtcatg cgcaacttga
    22321 ccatcgataa cctgaccgtc agccacgcta attacgccat tttacgccag ggatttcata
    22381 accagattat cggtgccaac atcaccaatt gtaagttcag cgacttacag ggcgacgcca
    22441 ttgaatggaa tgtagcaatc aatgatcgcg acattttgat atccgaccat gtcatcgagc
    22501 gcatcaactg tactaacggc aaaatcaact ggggtattgg catagggctg gcaggaagta
    22561 cttacgataa taattatccg gaaaaccagg cagtgaaaaa ctttgtcgtg gcgaatatca
    22621 cgggatcgga ttgtcggcaa ctgatccatg ttgaaaatgg taaacatttt gttatccgta
    22681 atatcaaagc tcgtaatatc acgccggatt tcagtaagaa agcaggtatt gataacgcca
    22741 cagttgctat ttatggttgt gacaactttg tgattgataa tattgaaatg attaatagtg
    22801 ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata ccgcagaatt
    22861 tccgggtgaa tgatattcaa ctggataata ctcatcttgc ttataaattg cgcggcattc
    22921 aaatctccgc cgggaatgct gtctcctttg tggcgctaac taacattgag atgaagcgtg
    22981 cgtcgctgga gttacacaac aagccgcaac atctttttat gcgcaatatc aatgtgatgc
    23041 aggaatcctc agttggacca gcattgagta tgaactttga catgcgcaaa gacgttcgag
    23101 gcgtgtttat ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg
    23161 aaaaagggca aagctccgtc gatatcgaca ggattaatca ccatattgtt aatgtggaaa
    23221 agattaactt tagattgccg gaacggagag agtagatttg cgaccattcc tggaaaaatg
    23281 tagtcatact taggaacaat gctactgcaa tccacaacga agcggcgtaa catcacaagt
    23341 aattcagtaa tcaattcagg ttaattgatg ctggcgaaaa aaatcgaaca agctataatt
    23401 cagcaaccat tttacaggtg gatgaaataa tgacgaattt aaaagcagtt attcctgtag
    23461 cgggtctagg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga
    23521 tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag
    23581 aaatcctcct ggtaactcac gcgtccaaaa acgcggtcga aaaccacttc gacacctctt
    23641 atgaattaga atctcttctt gaactgcgcg tgaagcgtca actgttggcg gaagtacagt
    23701 ccatctgtcc gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttag
    23761 gccactccat tttgtgtgcg cgacctgcca ttggtgacaa cccatttgtc gtggtactgc
    23821 cagacgttgt gatcgacgat gccagcgccg acccgctacg ttacaacctt gccgctatga
    23881 ttgcgcgctt caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg ccgggtgacc
    23941 tctctgaata ctccgtcatc cagactaaag agccgctgga ccgtgagggt aaagtcagcc
    24001 gcattgttga atttatcgaa aaaccggatc agccgcagac gctggactca gacatcatgg
    24061 ccgttggtcg ctatgtgctt tctgccgata tttggccgga acttgaacgc actcagcctg
    24121 gtgcatgggg acgtattcag cttactgatg ccattgccga actggcgaaa aaacagtccg
    24181 ttgatgccat gctgatgacc ggcgacagct acgactgcgg taaaaaaatg ggttatatgc
    24241 aagcgttcgt gaagtatgga ctacgcaacc tcaaagaagg ggcgaagttc cgtaaaggga
    24301 ttgagaagct gttaagcgaa taatgaatat ctgaccggat gtaacggttg ataagaaaat
    24361 tataacggca gtgaagattc gcagtaaaag taatttgttg cgaatattcc tgccgttgtt
    24421 ttatataaac catcagaata acaatgagtt agcagtaggg ttttagtcaa agttttccag
    24481 gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt tttcgggttt
    24541 agcacgagtg ggttacgctc gtcacatcgt ggaaatgtac gcagtgctct ggtagctgtt
    24601 aagccagggg cggtagcgtg cattaatgca tctattaatc aaatctggag cagtctattt
    24661 cacagcatgc tctctggcta tatggaataa aaaagtgaag atacttgtta ctggtggcgc
    24721 aggatttatt ggttctgctg ttgttcgtca cataataaat aatacgcaag atagtgttgt
    24781 taatgtcgat aaattaacat acgccggaaa cctggaatca ctcgctgaaa tttctgattc
    24841 tgaacgttat tcatttgagc atgcagatat ctgcgatgcc gaagcgatgg ctcgtatttt
    24901 cgcacagcac cagccagacg cggtgatgca cctggcagca gagagccacg ttgaccgctc
    24961 aataactggc cctgcggcat ttattgaaac caatattgtg ggtacttatg ttcttttaga
    25021 agcggcgcgc aattattggt ctggtctgga tgatgaaaag aaaaaaaact tccgttttca
    25081 tcatatttct actgatgagg tgtatggtga cttaccccat ccggatgaag taaatagcaa
    25141 tgaaacgttg ccgctattta cggaaacgac agcatacgcg ccaagtagtc catattctgc
    25201 ttctaaagct tccagcgatc atttggttcg cgcatggaaa cgtacttatg gtttaccgac
    25261 cattgtgact aattgctcga acaactatgg tccttatcat ttcccggaaa agcttattcc
    25321 actggttatt cttaattcac tggaaggtaa ggcattacct atttatggca aaggagatca
    25381 gatccgcgac tggttgtatg tagaggatca tgctcgagcg ttatataccg tcgtaaccga
    25441 aggtaaagcg ggcgaaactt ataacattgg tggacacaac gaaaagaaaa acatcgacgt
    25501 agtgttcact atttgtgatt tgttggatga gatagtcccg aaagagaaat cttatcgtga
    25561 gcaaattacc tatgttgctg atcgcccagg gcatgatcgc cgttatgcaa ttgatgccga
    25621 taaaattagc cgcgaattgg gctggaaacc gcaagaaacg tttgagagcg gtattcgtaa
    25681 aacggtggaa tggtatctgg ctaatacaaa ttgggttgag aatgtgaaaa gcggtgctta
    25741 tcagtcgtgg attgaacaga actatgaggg ccgccagtaa tgaatatcct cctattcggc
    25801 aaaacagggc aggtaggttg ggaactgcag cgtgctctgg caccgctggg taatctgatt
    25861 gctcttgatg ttcactccac tgattattgt ggtgatttta gtaatcccga aggtgtagct
    25921 gaaactgtca aaaaaattcg ccctgatgtt attgttaatg cggctgctca cactgcagta
    25981 gacaaagcgg aatcagaacc ggaatttgca caattactta acgcgacaag tgtcgaagcg
    26041 attgcaaaag cagccaatga agtcggcgct tgggttattc actactctac tgactacgta
    26101 tttccgggaa ccggtgaaat accgtggcag gagacggatg ctacagcacc tttgaatgtt
    26161 tatggtgaaa ctaaactagc cggagaaaaa gcgttacaga aacattgcgc gaagcatatt
    26221 attttccgta ccagctgggt atatgcaggt aaaggaaata acttcgccaa aacaatgttg
    26281 cgtctggcaa aagagcgcga agaactggct gtgataaacg atcagtttgg cgcaccaaca
    26341 ggtgccgaat tgctggctga ttgcaccgct catgccattc gcgtagcagt agacaaacca
    26401 gaagttgctg gtttgtacca tctggttgct ggcggtacaa caacctggca cgattatgcc
    26461 gctctggtat ttgaagaagc gcgcaaagca gggattaacc ttgctcttaa caaacttaac
    26521 gccgtgccaa caacggccta tcccacacca gcccgtcgac cccataactc tcgcctcaat
    26581 acagaaaagt ttcagcagaa ctttgcgctt gtcttgcctg actggcaggt gggtgtgaaa
    26641 cgaatgctca acgaattatt tacgactacg gcaatttaac aaatttttgc atctcgctca
    26701 tgatgccaga gcgggatgaa ttaaaaggaa tggtgaaatg aaaacgcgta aaggtattat
    26761 tctggctggt ggttccggca ctcgtcttta tcctgtgacg atggcagtaa gtaaacaact
    26821 gctgccgatt tatgataagc cgatgattta ttatccgctt tcaacgctta tgttagcggg
    26881 tattcgcgat attcttatta tcagtacgcc acaggatacg ccgcgtttcc aacaattgtt
    26941 gggggacggg agccagtggg ggcttaatct acagtataaa gtacaaccga gtccggatgg
    27001 cctggcgcaa gcgtttatca ttggtgaaga ctttattggt ggcgatgatt gtgcacttgt
    27061 gcttggtgat aatatcttct atggacacga cttgccgaaa ttaatggaag ctgctgttaa
    27121 caaagaaagc ggtgcaacgg tatttgctta tcacgttaat gatcctgaac gttatggtgt
    27181 cgtggagttt gataataacg gtactgcaat tagcctggaa gaaaaaccgc tggaaccaaa
    27241 aagtaactat gcggttactg ggctttattt ctatgacaat gacgttgtgg aaatggcgaa
    27301 aaaccttaag ccttctgccc gaggtgaact ggaaattacc gatattaacc gtatttatat
    27361 ggaacaagga cgtttgtctg tcgctatgat ggggcgtggc tatgcatggc tggatacagg
    27421 gacgcatcaa agtcttattg aagcaagcaa cttcattgcc accattgaag agcgccaggg
    27481 actaaaggtt tcctgtccgg aagaaattgc ttatcgtaaa gggtttattg atgctgagca
    27541 ggtaaaagta ttagccgaac cgttgaagaa aaatgcttat ggtcagtatc tgctcaaaat
    27601 gattaaaggt tattaataag atgaacgtaa ttaaaactga aattcctgat gtgctgattt
    27661 ttgaaccaaa agtttttggg gatgaacgtg gcttcttttt tgagagtttt aatcagagga
    27721 tttttgaaga agcagtaggt cgtaaggttg agtttgttca ggataaccat tctaagtcca
    27781 gtaaaggtgt tttacgtggt cttcattatc agttagaacc ttatgctcaa ggaaaactgg
    27841 tgcgctgtgt tgttggcgag gtttttgatg ttgcggttga tattcgtaaa tcgtcaccta
    27901 catttgggaa atgggttggg gtgaatttgt ctgctgagaa taagcgtcag ttgtggattc
    27961 ctgagggatt tgcacatggt tttttggtgc tgagtgattt agcagaagtt ttatataaaa
    28021 cgaatcaata ttatgctcca tcacatgaaa aaaatattat atggaatgac ctcttgctta
    28081 atattaaatg gccgagtaca gcactgatca ctctgtctga taaggatgca aatggggaaa
    28141 gatttgaact aagtgagttt tgaaatgtct ctcttaaaac atagtatatg gaatgttgcg
    28201 ggctacttta taccaacatt aattgcaatt cccgcctttg gattaattgc gaggaaaatt
    28261 ggtgtagaac tatttggttt gtatacgtta gcaatgattt ttatagggta tgcaagtata
    28321 tttgatgctg ggttaacaag agctgttgtg cgtgaaatag cattactaaa aaacagagtg
    28381 gacgattgta atacgataat agtaacttct attatcgctg tgatattttt agggtttatc
    28441 ggaggcgggg gagtgtttct gcttaaaggc gatattattg aactgttaaa tatctcacca
    28501 atatattacg ccgattcgat aaagtctcta gtattattat catctctgat acctgtattc
    28561 ttagtcacgc aaatactatt agcagagctt gagggtcggg aatattttgg gattctaaat
    28621 atacaaaaaa gtgtagggaa ttctttaatt gcagggttac ctgcattatt tgttttaatt
    28681 aatcaaacgc ttttttctgc aattattggt gtagcgattg caagagttat atgcttgtgg
    28741 ttaagctaca ttatgagcag ggaaagaata actatcgata tctcattttt ttcaataact
    28801 gttttaaagc ggttatttag atatggcggg tgggtaacta taagtaacat aatatctcct
    28861 atattagcga gtatggatag atttattcta tcccatatcc agggagcatc aaaaatatca
    28921 ttctatacag tccctaatga gctggtaact aggcttggaa tagttccagg ctctcttggg
    28981 aaagctgttt ttccaaaatt aagtcatgca aggaatttta cagcgtcata tgcagagcaa
    29041 aaaaaagctt atatattaat gactgtcatt gtaatgcctt tggttttatt tgtatattat
    29101 tacgcaaagt ttattttaac attgtggatg ggggctgagt atgcagggat ttcggtcgaa
    29161 atattacgga ttatgcttat agggtatatt tttaactgtt attcacaaat ctcttttgcc
    29221 aacatacagg cctttggaaa agcaaaatac actgcataca tccatatgat ggaatttatt
    29281 ccttatttga taatgttata tataatttca aaggaatatg gggttattgg tgttgcgtgg
    29341 ttatggacaa ttcgagtaa
//
LOCUS       DAJOTB010000051        28240 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJOTB010000051 DAJOTB010000000
VERSION     DAJOTB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.051"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            346..1116
                     /locus_tag="OHN28_002159"
     CDS             346..1116
                     /locus_tag="OHN28_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCQ2297556.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            1131..2072
                     /locus_tag="OHN28_002160"
     CDS             1131..2072
                     /locus_tag="OHN28_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2297557.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            2123..3409
                     /locus_tag="OHN28_002161"
     CDS             2123..3409
                     /locus_tag="OHN28_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2297558.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3406..3693
                     /gene="fixX"
                     /locus_tag="OHN28_002162"
     CDS             3406..3693
                     /gene="fixX"
                     /locus_tag="OHN28_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCQ2297559.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKRDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3752..5083
                     /locus_tag="OHN28_002163"
     CDS             3752..5083
                     /locus_tag="OHN28_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2297560.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWTLPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            5191..5721
                     /gene="kefF"
                     /locus_tag="OHN28_002164"
     CDS             5191..5721
                     /gene="kefF"
                     /locus_tag="OHN28_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCQ2297561.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5714..7576
                     /gene="kefC"
                     /locus_tag="OHN28_002165"
     CDS             5714..7576
                     /gene="kefC"
                     /locus_tag="OHN28_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCQ2297562.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7768..8247
                     /gene="folA"
                     /locus_tag="OHN28_002166"
     CDS             7768..8247
                     /gene="folA"
                     /locus_tag="OHN28_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCQ2297563.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8333..8566
                     /locus_tag="OHN28_002167"
     CDS             8333..8566
                     /locus_tag="OHN28_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HCQ2297564.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8569..8853
                     /locus_tag="OHN28_002168"
     CDS             8569..8853
                     /locus_tag="OHN28_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HCQ2297565.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIK"
     gene            complement(8880..9728)
                     /gene="apaH"
                     /locus_tag="OHN28_002169"
     CDS             complement(8880..9728)
                     /gene="apaH"
                     /locus_tag="OHN28_002169"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCQ2297566.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(9735..10112)
                     /gene="apaG"
                     /locus_tag="OHN28_002170"
     CDS             complement(9735..10112)
                     /gene="apaG"
                     /locus_tag="OHN28_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCQ2297567.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(10115..10936)
                     /gene="rsmA"
                     /locus_tag="OHN28_002171"
     CDS             complement(10115..10936)
                     /gene="rsmA"
                     /locus_tag="OHN28_002171"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCQ2297568.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVGVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10933..11922)
                     /gene="pdxA"
                     /locus_tag="OHN28_002172"
     CDS             complement(10933..11922)
                     /gene="pdxA"
                     /locus_tag="OHN28_002172"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCQ2297569.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11922..13208)
                     /gene="surA"
                     /locus_tag="OHN28_002173"
     CDS             complement(11922..13208)
                     /gene="surA"
                     /locus_tag="OHN28_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCQ2297570.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(13261..15615)
                     /gene="lptD"
                     /locus_tag="OHN28_002174"
     CDS             complement(13261..15615)
                     /gene="lptD"
                     /locus_tag="OHN28_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCQ2297571.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15870..16685
                     /gene="djlA"
                     /locus_tag="OHN28_002175"
     CDS             15870..16685
                     /gene="djlA"
                     /locus_tag="OHN28_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCQ2297572.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGSGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(16835..17494)
                     /gene="rluA"
                     /locus_tag="OHN28_002176"
     CDS             complement(16835..17494)
                     /gene="rluA"
                     /locus_tag="OHN28_002176"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCQ2297573.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(17506..20412)
                     /gene="rapA"
                     /locus_tag="OHN28_002177"
     CDS             complement(17506..20412)
                     /gene="rapA"
                     /locus_tag="OHN28_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCQ2297574.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(20577..22928)
                     /gene="polB"
                     /locus_tag="OHN28_002178"
     CDS             complement(20577..22928)
                     /gene="polB"
                     /locus_tag="OHN28_002178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCQ2297575.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(23003..23698)
                     /gene="araD"
                     /locus_tag="OHN28_002179"
     CDS             complement(23003..23698)
                     /gene="araD"
                     /locus_tag="OHN28_002179"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2297576.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(23813..25315)
                     /gene="araA"
                     /locus_tag="OHN28_002180"
     CDS             complement(23813..25315)
                     /gene="araA"
                     /locus_tag="OHN28_002180"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCQ2297577.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(25326..27026)
                     /gene="araB"
                     /locus_tag="OHN28_002181"
     CDS             complement(25326..27026)
                     /gene="araB"
                     /locus_tag="OHN28_002181"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCQ2297578.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELCVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRNIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEVWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            27365..28210
                     /gene="araC"
                     /locus_tag="OHN28_002182"
     CDS             27365..28210
                     /gene="araC"
                     /locus_tag="OHN28_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCQ2297579.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWVNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         6300 a         7375 c         7787 g         6778 t
ORIGIN      
        1 taagctgcat tctgtgattg ttgtcacatt tttgtttcgg gtgaatagag ggcatttttt
       61 cgttaatttt gattattaat cagtttgtta tgttctgttg tgggtaaaaa aatagcatct
      121 gactttcaat attggtgatc cataaaacaa tattgaaaat ttctttttgc tacgccatgt
      181 tttcaatatt ggtgaggagc ttaacaatat tgaaagttgg atttatctgc gtgtgacatt
      241 ttcaatattg gtgattaaag ttttatttcc aaattaaagg gcgtgatatc tgtaattaac
      301 accaccgata tgaacgacgt ttccttcatg atttctggag atgcaatgaa gattattact
      361 tgctataagt gcgtgcctga tgaacaggat attgcggtca ataatgctga tggttcgtta
      421 gacttcagca aagccgatgc caaaataagc caatacgatc tcaacgctat tgaagcggct
      481 tgccagctaa agcaacaggc agcagaggcg caggtgacgg ccttaagtgt gggcggtaaa
      541 gccctgacca acgccaaagg gcgtaaagat gtgctatcgc gcggtccgga tgaactgatt
      601 gtggtgattg atgaccagtt cgagcaggca ctgccgcaac aaacggcgag cgtacttgct
      661 gcagccgctc agaaagcagg ctttgatttg atcctctgtg gcgatggttc ttccgacctt
      721 tatgcccagc aggttggttt gctggtaggc gaaatcctta atattccggc agttaacggc
      781 gtcagcaaaa ttatctccct gacggcagat accctcaccg ttgagcgcga actggaagat
      841 gaaacagaaa ccttaagcat tccgctgcct gcggttgttg ctgtttccac tgatatcaac
      901 tccccacaaa ttccttcgat gaaagccatt ctcggcgcgg cgaaaaagcc agtccaggta
      961 tggtcggcgg cggatatcgg ttttaacgca gaggcagcct ggtcagaaca acaggttgcc
     1021 gcgccgaaac agcgcgagcg tcagcgcatc gtgattgaag gcgacggcga agaacagatc
     1081 gccgcatttg ctgaaaatct tcgcaaagtc atttaattac aggggatgct atgaacacgt
     1141 tttctcaagt ctgggtattc agcgataccc cttctcgtct gccggaactg atgaacggtg
     1201 cgcaggcttt agctaatcaa atcaacacgt tcgtcctcaa tgatgccgac ggcgcacagg
     1261 caatccagct cggcgctaat catgtctgga aattaagcgg caaaccagac gagcggatga
     1321 tcgaagatta cgccggtgtc atggctgaca ctattcgcca gcacggcgca gacggtctgg
     1381 tgctgttgcc aaacacccgt cgcggcaaat tactggcggc gaaactgggt taccgcctta
     1441 acgcggcggt gtctaacgat gccagcgctg ttagcgtgca ggacggtaaa gcgacagtga
     1501 aacacatggt ttacggcggt ctggcgattg gcgaagaacg cattgccacg ccgtatgcgg
     1561 tactgaccat cagcagcggc acgttcgatg cggctcagcc agacgcgtca cgcactggcg
     1621 aaacgcacac cgtggagtgg caggctccgg ctgtggcgat tacccgcacg gcaacccagg
     1681 cgcgtcagag caacagcgtc gatctcgaca aagcccgcct ggtggtcagc gtcggtcgcg
     1741 gtattggcag caaagagaac attgcgctgg cagaacagct ttgtaaggcg ataggtgcgg
     1801 agttggcctg ttctcgtccg gtggcggaaa acgaaaaatg gatggagcac gaacgctatg
     1861 tcggtatctc caacctgatg ctgaaacctg aattgtacct ggcggtgggg atctccgggc
     1921 agatccagca catggttggc gctaatgcgt cgcaaaccat tttcgccatc aacaaagata
     1981 aaaatgcgcc gatcttccag tacgcggatt acggcattgt tggcgacgcc gtgaagatcc
     2041 ttccggcact gaccgcagct ttagcgcgtt gatccactct ggcagggctg cattccggcc
     2101 ctgccgctga cagggagctc ttatgtccga agatatcttt gacgccatca tcgtcggtgc
     2161 agggcttgcc ggttcggttg ccgcactggt gctcgcccgc gaaggtgcgc aagtgttagt
     2221 tatcgagcga ggcaattccg caggtgccaa gaacgtcact ggcgggcgtc tctatgccca
     2281 cagtctggaa cacattattc ctggtttcgc tgactccgcc cccgtagaac gcctgatcac
     2341 ccatgaaaaa cttgcgttta tgacggaaaa gtcagcgatg actatggact actgcaatgg
     2401 cgacgaaacc tctccatccc agcgttctta ctccgttttg cgcagtaaat ttgatgcctg
     2461 gctgatggag caggccgaag aagcgggcgc gcagttaatt accgggatcc gcgtcgataa
     2521 cctcgtacag cgcgatggca aagtcgtcgg tgtagaagcc gatggcgatg tgattgaagc
     2581 gaaaacggtg atccttgctg atggggtgaa ttccatcctt gccgaaaagc tggggatggc
     2641 aaaacgcgtt aaaccgacgg atgtggcggt tggcgtgaag gaactgatcg agttaccgaa
     2701 gtcggttatt gaagaccgtt ttcagttgca gggtaatcag ggcgcggctt gcctgtttgc
     2761 gggatcaccc accgatggcc tgatgggcgg tggcttcctt tataccaatg aaaataccct
     2821 gtcgctgggg ctggtttgtg gtctccatca tctgcatgac gcaaaaaaat cggtgccgca
     2881 aatgctggaa gatttcaaac aacatccggc cgttgcaccg ctgatcgcgg gcggcaagct
     2941 ggtggaatat tccgctcacg tagtgccgga agcaggcatc aacatgctgc cggagctggt
     3001 cggtgacggc gtattgattg ccggtgatgc tgccggaatg tgtatgaacc tcggttttac
     3061 cattcgcggt atggatctgg cgattgccgc cggggaagca gcagcaaaaa ctgtgctttc
     3121 agcgatgaaa agcgacgatt tcagtaagca aaaactcgcg gaatatcgtc agcatcttga
     3181 gagtggcccg ctgcgcgata tgcgcatgta ccagaaacta ccggcgttcc ttgataaccc
     3241 acgcatgttt aacggctacc cggaactggc ggtgggcgtg gcgcgtgacc tgttcaccat
     3301 tgacggcagt gcgccggaac tgatgcgcaa gaaaatcctc cgccacggca agaaagtggg
     3361 cttcatcaat ctgatcaagg atggcatgaa aggagtgacc gttttatgac ttctcccgtc
     3421 aatgtggacg tcaaactggg cgtcaataaa ttcaatgtcg atgaagagca tccgcacatt
     3481 gttgtgaagg ccgatgccga taaacaggcc ctggagctgc tggtgaaagc gtgccccgca
     3541 ggtctgtaca agaagcggga tgacggcagt gtgcgcttcg attacgccgg atgtctggag
     3601 tgcggcacct gtcgcattct ggggctgggg tcggcgctgg aacagtggga atacccgcgc
     3661 ggcacctttg gtgtggagtt ccgttacggc tgatgttggt ttgatacgta atgccgtact
     3721 gactctcatt gcaaaaaaac aggaataacc catgcaaccg tccagaaact ttgatgatct
     3781 taaattctcc tctattcacc gccgcatttt gctgtgggga agcggtggtc cgtttctgga
     3841 tggttatgta ctggtaatga ttggcgtggc gctggagcaa ctgactccgg cgctgaaact
     3901 ggacgctgac tggattggct tgctgggcgc gggaacgctc gccgggctgt tcgttggcac
     3961 atcgctgttt ggctatatct ccgataaagt cggacggcgc aaaatgttcc tcattgatat
     4021 catcgccatc ggcgtgatat cggtggcaac gatgtttgtc tcatcccccg tcgaactgtt
     4081 ggtgatgcgg gtacttatcg gcattgtcat tggtgcggat tatcccatcg ccacctcaat
     4141 gatcaccgag ttctccagta cccgtcagcg ggcgttttcc atcagcttta ttgccgcgat
     4201 gtggtatgtc ggcgcgacct gcgccgattt ggtcggctat tggctttatg atgtggaagg
     4261 cggctggcgc tggatgctgg gtagcgcagc gattccctgt ttgttgattt tgattggtcg
     4321 attcgaactg cctgaatctc cccgctggtt gttacgcaaa gggcgagtaa aagagtgcga
     4381 agagatgatg ataaaactgt ttggcgaacc ggtggctttc gaggaagagc agccgcagca
     4441 aacccgtttt cgcgatctgt ttaatcgccg ccattttcct tttgttctgt ttgttgccgc
     4501 tatctggacc tgccaggtga tccccatgtt cgccatttac acctttggcc cgcaaatcgt
     4561 tggtttgttg ggattggggg ttggcaaaaa cgcggcactg gggaacgtgg tgattagcct
     4621 gttctttatg ctcggctgta ttccgccgat gctgtggcta aacactgccg gacggcgtcc
     4681 attgttgatt ggcagctttg ccatgatgac gctggcgctg gcggttttgg ggctgatccc
     4741 ggatatgggg atctggctgg tagtgatggc ctttgcggtg tatgcctttt tctctggcgg
     4801 gccgggtaat ttgcagtggc tctatcctaa tgaactcttc ccgacggata tccgcgcctc
     4861 tgccgtgggc gtgattatgt ccttaagccg tattggcact attgtatcga cctggacact
     4921 gccgatcttt atcaataatt acggtatcag taacacgatg ctaatggggg cgggtatctc
     4981 gctatttggc ttgttgattt ccgtagcgtt tgccccggag actcgaggga tgtcactggc
     5041 gcagaccagc aatatgacga tccgcgggca gagaatgggg taatttgttc agatttctct
     5101 cttttctgaa tcaatattat tgactataag ctgcgtgaat atatgactac actttgtggg
     5161 aaaacaaagg cgtaatcacg cgggctacct atgattctta taatttatgc gcatccatat
     5221 ccgcatcatt cccatgcgaa taaacggatg cttgaacagg caaggacgct ggaaggcgtc
     5281 gaaattcgct ctctttatca actctatcct gacttcaata tcgacattgc cgccgaacag
     5341 gaggcgctgt ctcgtgccga tctgatcgtc tggcagcacc cgatgcagtg gtacagcatt
     5401 ccaccgcttc ttaaactttg gatcgataaa gttttctcgc acggctgggc ttacggtcac
     5461 ggcggcacgg cgctgcacgg caaacatttg ctgtgggcgg tgacgaccgg cggcggggaa
     5521 agccattttg agattggtgc gcatccgggc tttgatgtgc tgtcgcagcc gctacaggcg
     5581 acggcaatct actgcgggct gaactggctg ccaccgtttg ccatgcactg cacctttatt
     5641 tgtgacgacg aaaccctcga agggcaggcg cggcactata agcaacgtct gctggaatgg
     5701 caggaggcac atcatggata gccatacgct tattcaggcg ctgatttatc tcggttcggc
     5761 ggcgctgatt gttcccattg cggtacgtct cggtctggga tcggttcttg gctacctgat
     5821 cgccggctgc attattggcc cgtgggggct gcgactggtg accgatgccg aatctattct
     5881 gcactttgca gagattggcg tggtgctgat gctgtttatt atcggcctcg aactcgatcc
     5941 acaaaggctg tggaagctgc gtgcggcagt gttcggcggt ggcgcattgc agatggtgat
     6001 ttgcggcggc ctgctggggc tgttttgtat gttacttggg ctgcgctggc aggtcgcaga
     6061 attgatcggc atgacgctgg cgctctcatc aacggcgatt gccatgcagg cgatgaatga
     6121 acgcaatctg atggtgacgc aaatgggccg cagtgccttt gcggtgctgc tgttccagga
     6181 tatcgcggcg atcccactgg tggcgatgat cccgctactg gcagcgagta gtgcctcgac
     6241 gacgatgggc gcatttgttc tctcggcgtt aaaagtggcg ggcgcgctgg tgctggtggt
     6301 attgctgggg cgctatgtca cgcgtccggc actgcgtttt gttgcccgct ctggcttgcg
     6361 ggaagtgttt agtgccgtgg cgttattcct cgtgtttggc tttggtttgc tgctggaaga
     6421 ggtcggcttg tcgatggcga tgggcgcgtt tctggcgggc gtactgctgg caagctcgga
     6481 ataccgtcat gcgctggaaa gcgatatcga accatttaaa ggtttgctgc tggggctgtt
     6541 tttcatcggc gttggcatgt cgatagactt tggcacgctg cttgaaaatc cgttgcgcat
     6601 tgtcattttg ctgctcggtt tcctcatcat caaaatcgcc atgctgtggc tgattgcccg
     6661 gccgttgcag gtgccaaata aacagcgtcg ctggtttgcg gtgctgttag ggcagggcag
     6721 tgagtttgcc tttgtagtat ttggtgcggc gcagatggcg aatgtgctgg agccagagtg
     6781 ggcgaaatcg ctgaccctgg cggtggcact gtcgatggcg gcaacgccga ttctgctggt
     6841 gatcctcaat cgccttgagc aatcttctac tgaagaggcg cgtgaagccg atgagatcga
     6901 cgaagaacag ccgcgggtaa tcattgccgg attcggtcgt tttgggcaga taaccggacg
     6961 tttactgctc tccagcgggg tgaaaatggt ggtgctcgat cacgacccgg accatatcga
     7021 aacgctgcgt aaattcggta tgaaagtgtt ttatggcgat gccacgcgga tggatttact
     7081 ggaatctgcc ggagcggcga aagcggaagt gctgattaac gccatcgacg atccgcaaac
     7141 caacctgcaa ctgacggaga tggtgaaaga acatttccca catttgcaga ttattgcccg
     7201 cgcccgcgat gtcgaccact acattcgttt gcgtcaggca ggcgttgaaa agccggagcg
     7261 tgaaaccttc gaaggtgcgc tgaaaaccgg gcgtctggca ctggaaagtt taggtctggg
     7321 gccgtatgaa gcgcgagaac gtgccgatgt gttccgccgc tttaatattc agatggtgga
     7381 agagatggca atggttgaga acgacaccaa agcccgtgcg gcggtctata aacgcaccag
     7441 cgcgatgtta agtgagatca ttaccgaaga ccgtgaacat ctgtcattaa ttcaacgaca
     7501 tggctggcag ggaaccgaag aagggaaaca tactggcaat atggcggatg aaccggaaac
     7561 gaagccctca tcctaataaa gagtgacgta aatcacgctt tacagctaac tgtttgtttt
     7621 tgtttcattg taatgcggcg agtccaggga gagagcgtgg actcgccagc agaatataaa
     7681 attttcctca acatcatcct cgcaccagtc gacgacggtt tacgctttac gtatagtggc
     7741 gacaattttt tttatcggga aatctcaatg atcagtctga ttgcggcgtt agcggtagat
     7801 cgcgttatcg gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt
     7861 aaacgcaaca ctttaaataa acccgtgatt atgggccgcc atacctggga atccatcggt
     7921 cgtccgttgc caggacgcaa aaatattatc ctcagcagtc aaccgggtac ggacgatcgc
     7981 gtaacgtggg tgaagtcggt ggatgaagcc attgcggcgt gtggtgacgt accagaaatc
     8041 atggtgattg gcggcggtcg cgtttatgag cagttcctgc cgaaagcgca aaaactgtat
     8101 ctgacgcata tcgacgcgga agtggaaggc gacacccatt tcccggatta cgagccggat
     8161 gactgggaat cggtattcag tgaattccac gacgccgatg cgcagaactc tcacagctat
     8221 tgctttgaga ttctggagcg gcggtaattt cgtatattag aattccggca tcactgatac
     8281 gtgcagatat ccgtgtatac tagacgtata aattgtacag gagcacgaga tcatgactgc
     8341 aaaacgtacc acacaaagtg tgaccgtcac cgtcgaccgt gagttagtca atcgcgctcg
     8401 tgatgcaggc ttaaatatga gcgccaccct tacggttgcg ctcaatgctg aacttaaaaa
     8461 acatgcagca acacgttggc gtgaagagaa cgcagaagct atcgctgcgt taaatcaatt
     8521 ggctgatgaa accggatgtt tctctgatga gtaccggagc ttctagcgat gcaatttacg
     8581 gtataccgca gtcgcggcag gaacgccgcg tttccctttg ttattgatgt taccagcgac
     8641 attattggtg agattaatcg ccgtatcgtt attccattaa cacctattga acgatttagc
     8701 cgtattcgcc caccagaacg gcttaaccca atccttttac tgatagacgg caaagaatat
     8761 gtgctcatga cgcatgagac tgcaactgtt ccagttaacg cgctgggaac gaaattttgc
     8821 gatgccagcg cgcaccgaac gttgattaaa tgagcattag attttatgct cgacgggatt
     8881 taagacgcta ccgcctctcc ctcactcaaa tccttatgcc ggttcgacgg ctggacaaaa
     8941 tactgtttat cttcccagcg caggcaggtt aatgtaccac cccagcagca gccggtatcc
     9001 agcgcgtaaa taccttccgg cgtacctttg ccttccagcg atgcccagtg accaaaggcg
     9061 atgttgtact cttcagcgac ggggccagga atcgcaaacc acggtttcag tggggcaggg
     9121 gcctcttccg gcgattcttt gctgtacata tccagttgac cgttcgggaa gcaaaaacgc
     9181 atacgggtaa aggcgttggt gataaaacgc agtcttccca gcccccgcaa ttccggtgac
     9241 cagttatttg gcatatcgcc gtacatggca tcaaggaaga agggatagga gtcactcgac
     9301 agcaccgctt ctacatcgcg cgcgcactct ttggcggtct gcagatccca ctgcggcgtg
     9361 atcccggcgt gggccatcac caactttttc tcttcgtcga tttgcagcaa aggctggcga
     9421 cgcagccagt taagcagctc gtcggcatcc ggcgcttcca gcagcggtgt caggcgatct
     9481 ttcggtttat tgcggctgat cccggcaaat accgccagca gatgcagatc gtgattaccc
     9541 agcaccagac gtacgctgtc gcctaaggat ttcacatagc gcagaacatc cagcgaaccc
     9601 gggccgcgcg cgaccagatc gcccgtcagc cagagggtat ctttcccagg ggtaaattct
     9661 actttatgca gcaatgcgat cagttcatcg taacaaccat gaacgtcgcc aataaggtat
     9721 gtcgccatat tcttttaatg aatgagtgtg ggaacggcga gtcggaatac gggaatgtcg
     9781 atgctgaaag ggacgccatt ttcatcgatc atttcgtagt gaccctgcat ggtgcccagc
     9841 ggggtttcaa tgattgcacc gctggtgtac tggtactctt cgccaggcgc gataagtggc
     9901 tggacgccaa ccactccttc gccctggact tcggtttcac ggccattgcc attggtgatc
     9961 agccagtaac gccccaacaa ctgcactggc gctcgcccca gattgcgtat ggttacggta
    10021 taagcaaaaa cgtaacgttc attatcaggt gaagattggg cctcaatgta gacgctttga
    10081 acctgaatac acactcgggg cgaattgatc atcgttaact ctcctgcaaa ggcgcgttct
    10141 ccgccagata gttcgccatc tggcaatatt gcgcgacaga gatattttcc gctcgcatcg
    10201 ccgggtcgat ccccattccc gttaacaccc cgacgctaaa caggttgccg aggctgttac
    10261 gaatggtttt acgacgctgg ttaaaggctt cggtggtgat gcggctcaac acgcgaacat
    10321 ctttaaccgg gtgaggcatc gttgcatgag gaaccaggcg cacgacggcg gaatccactt
    10381 tgggtggtgg tgtaaaggct gacggcggta cttccagcac cgggatcacg ttgcaatagt
    10441 attgcgccat gacgcttaat cgaccatacg ccttgctgtt cggtcctgca accagacgat
    10501 tcaccacttc tttttgcaac ataaagtgca tgtcggcaat ggcatcagta tagctaaaca
    10561 gatggaacat caacggcgtg gagatgttat aaggcaggtt gccgaaaaca cgcagcggct
    10621 ggcccatttt cgcggccagt tcaccaaagt taaaggtcat cgcatcctgc tgataaatcg
    10681 tcagtttcgg gcctaagaat ggatgcgttt gcagacgtgc cgccagatcg cggtcaagtt
    10741 cgatgaccgt cagctggtcc agacgttcgc cgaccggttc ggtcaatgcc gccagaccgg
    10801 ggccgatttc gaccatcgcc tggcccttct gcgggttaat ggcagaaaca atgctgtcga
    10861 tcacgaactg atcgttgaga aagttttgcc cgaagcgttt acgggctaag tggccctggt
    10921 ggactcgatt attcattggg tgttaacaat cattttgatg gcgagattaa gcgccgtaat
    10981 aaaactgccg acatcggctt taccacgtcc cgccagttca agcgcggtgc cgtggtccac
    11041 tgatgtgcga ataaagggca ggcccagcgt aatgttcaca ccgcgcccga agccctggta
    11101 ttttagcacg ggaagaccct gatcgtggta catcgccagc acggcgtcgg cgttatcgag
    11161 atatttcggc tgaaacaggg tatcggcagg cagcggcccg ttgagtttca tcccctgttc
    11221 ccgcagctcg tcgagtaccg gaataatggt gtctatctct tccgtaccca tatgaccgcc
    11281 ttcgcccgcg tgcggattca gcccgcagac cagaatgcgc ggttcggcaa taccaaattt
    11341 ggtccgcaaa tcgtgatgca aaatagcaat cacttcgtgc aaaagggcag gggtgatagc
    11401 atctgcgata tcgcgcagcg gtaaatgcgt cgttgccagc gccacgcgaa gttcttcggt
    11461 cgccagcatc atcaccactt ttttcgcctg cgaacgctct tcgaaaaact cggtatgacc
    11521 ggtaaacgga atgcctgcgt cgttaatgac gcctttatgc acggggcctg tgatcagcgc
    11581 agcaaattca ccgttcagac agccatcaca cgctcgcgcc agcgtttcca ccacatagtg
    11641 cccattttca accgctaaca gccccacagt gacagattca cgtagcgcga caggaagtag
    11701 cgttaatgtg cccgcagttt gcggttgtgc aggggagttg ggggaataag ggcggagggt
    11761 gagcggcaaa ccgagcatcg ctgcccggtc ggtaaggaga gtggcatcgg cacaaacaac
    11821 cagttcgacc ggccactcac gctgtgcaag ctggacaatt aagtccgggc caatcccggc
    11881 gggctcgccg ggagtgatca caacacgttg ggttttaacc attagttgct caggatttta
    11941 acgtaggcgc tggcacgttg ttcctgcatc cagcttgctg cttcttccga gaacttacgg
    12001 ttcatcagca tgcggtatgc acgatctttc tgtgccgcgt cggttttatc gacattacgg
    12061 gtatccagca gttcgattaa atgccagccg aatgaagagt gaaccggtgc actcatttga
    12121 cctttgttca ggcgggtcag ggcgtcacgg aaggccggat cgaaaatatc tggtgtagcc
    12181 cagccgagat caccgccctg gttagcagag cctggatcct gagagaactc tttcgctgcg
    12241 gcagcaaaag tcgttttacc actcttgata tcggcagcaa tctgttccag tttcacgcgg
    12301 gcctgttcgt cagtcatgat cggcgacggt ttcagcagaa tatggcgagc atgaacttcg
    12361 gtcaccgaga tatttttgct ttcgccgcgc aggtcgttaa ctttcagaat atggaagcca
    12421 acgccggaac gaatcgggcc aacaatgtcg cctttcttcg cggtgcttaa tgcctgggcg
    12481 aagatccccg gcaactcctg aatacggccc cagcccatct ggccgccgtt cagcgcctgc
    12541 tggtcggcag aatgagcaat cgccagctta ccgaaatcag cgccgttacg cgcctgatcg
    12601 acaatcgcgc gcgcctggct ttccgcttcg ttcacctgat cagaggtcgg gttttccggc
    12661 agcgggatca ggatgtggct caggttcagc tcagtgctgg cgtcgttttg gttacccact
    12721 tgctgcgcca gggattcgac ttcctgcggc aggatggtga tgcgacgacg cacctcgttg
    12781 ttacgcactt cagagataat catctctttg cggatctggt tacgataggt gttgtagttc
    12841 agaccatcgt aagccagacg gctgcgcatc tgatccagcg tcatgttgtt ctgtttcgcg
    12901 atgttagcaa tcgcctgatc cagctgctca tcggagattt tcactcccat tttctgcccc
    12961 atctgcagga taatttgatc catgatcaaa cgttccatga tttggtggcg cagcgtcgcg
    13021 tcatcaggaa gttgttgcct tgcctgagca gcgttcagtt ttaccgactg cattaatcca
    13081 tcaacgtcgc tttccagcac gacgccgtta ttgacgacgg ctgcgacttt atcgactacc
    13141 tggggggcag cgaaactggt attcgcgatc atggcgatac cgagaagcag cgttttccag
    13201 ttcttcatac tttttccatt tcaattaacc gcactgcgga ttacgtggta aatcaacaaa
    13261 tcacaaagtg ttttgatacg gcagaatgtt cgaacgcagc atctcttgcg tacccagacc
    13321 gtagttggag ctcaggccgc gaagttcgat gttaaagccg attgcgttgt catataccgc
    13381 atgttgttta tcgttatccc aaccgttcag cttccgctcg taaccgacgc gaatcgcata
    13441 gcagcaggag ctgtattgca cacctaacat agagtcggct tgcttgttcg cattggtgtc
    13501 gtagtagtag gccccaacaa tggaccaacg atcggcaatt ggccagctgg cgacggcacc
    13561 tacctgcgaa ataccattct tatattgctc agcagtggaa tagtacttag gcagcgtagc
    13621 ctgaatatat tccgggctgg cgtaacggta attcagctgt accagacggt cttcatcacg
    13681 acggtattca atgctggagt tactggtcgc tacgttatcc agacgtgtat cgtactgaat
    13741 cccgccacgc aatccccaac gatcggagat gcgccagtaa gtatcgcctg cccataccag
    13801 tgagcccgtt ttgtcgtcat tctcccatgt tatgttgtca tcgccagtgc gagactccgt
    13861 gaaatagtag atttgaccaa cggaaatatt aaaacgttca acggcagcat catcatatat
    13921 gcgagatgtg acaccggtcg tcacctggtt agcggaggca atacggtcaa gaccgccgta
    13981 agtccggtcc cggaacaggc cggagtagtc agattgcagc agcgaagagt cgtagttata
    14041 gattttgctc tgatcgcgat atggcacata cagatactgc gcgcgtggtt ccagtgtctg
    14101 ggtatagccc ggcgccagca tttccatatc acgctcgaag accattctac cgtctacctt
    14161 gaactgcggc attacacggt tagcggactc tgccagcctg gtcgtgtttc tggagttata
    14221 ccagtcaaga ttggtttgct gataatgggt tgccagcaac ttcgcttcgg tattgatgct
    14281 gccccagtta ttagagagcg gcagattgat ggtcggttcc aggtgaacac gggttgcttc
    14341 aggcatgtcg tctctggtgt taacaaagtg cactgcctga ccgtaaatac gcgtatcaaa
    14401 cggaccaaca tcattctggt agtagttaac gtctaactgc ggctctgccg agtagctact
    14461 ggtgttctgc tcgctgaaga cctggaactg cttggttgaa acggtggcat tgaagttttg
    14521 caccgcatag ccaacgctga atttttgcgt tgcgtagccg tcggtactgg aaccgtattt
    14581 gttatcgaag tcattgaagt agctaggatc gctgactttg gtgtagtcga cgttgaaacg
    14641 ccacacctga tccatgaccc cggagtggtt ccagtagaac aaccaacgac gtgaactgtc
    14701 atcgttcggg tgttcatctt tatagacttt atctgaaggc agatagtcca gttccatcaa
    14761 gccagcgccc gcctgggaga ggtagcggaa ttcgttctcc cacatgatgt tgccacgacg
    14821 atgcatataa tgcggcgtga tggtggcatc catatttggc gcgatgttcc agtaatatgg
    14881 caggtagaac tcaaagtagt tggtggtggt gtacttggcg ttcgggatca agaaaccaga
    14941 gcgacgtttg tcacccaccg gcaactgcaa atacgggcta taaaagatcg gtaccggacc
    15001 caccttaaag cgggcgttcc agatctccgc aacttgttct tcgcggtcat gaataatttc
    15061 gctacctacc acgctccagg tgtcagaacc cggcagacag gaggtaaagc taccgttatc
    15121 cagaatggta tagcggtttt cgccacgttg tttcatcagg tccgctttac cgcgcccctg
    15181 gcgacccacc atctggtaat caccttccca gacgttggta tctttggtgt tcagattcgc
    15241 ccagcctttc ggccctttga ggatcacctg gttatcgtcg taatggacat taccgagcgc
    15301 atcaacggta cgtaccggct ccggttgtcc tggtgcctct ttttgatgga gctgcacttc
    15361 gtcggcctgc agacggctgt taccctgcat gatatccacg ctgccagtaa acacggcgtc
    15421 atccgggtag tcccctttcg cgtggtcagc attgatagtc acgggtaagt cattggtatc
    15481 gccctgtacc agaggacggt catagcttgg cacgcccaac atgcactgtg aggcgaggtc
    15541 ggctgccagt ccctgttgac tataaagggc ggtggcaatc atggtggcca ggagagtggg
    15601 gatacgtttt ttcatacgtt gattttattg ttccatcatc ggtaacgttg cgcgtgacaa
    15661 acggtcagag actaacgtac tcgtcatctc tacgctagtg ttaatcctgt ccgaatagcg
    15721 tcagtggtgt taggcacggc attgaatgac aggtatgata atgcaaatta taggcgatgt
    15781 cccacaattg accgcagccg gaaaacggta aaagcacctt tatattgtgg gagatagccc
    15841 tgatatccgt gtgtcgattt ggggaatata tgcagtattg gggaaaaatc attggcgtgg
    15901 ccgtggcctt actgatgggc ggcggctttt ggggcgtagt gttaggtctg ttaattggcc
    15961 atatgtttga taaagcccgt agccgtaaaa tggcgtggtt cgccaaccag cgtgagcgtc
    16021 aggcgctgtt ttttgccacc acttttgaag tgatgggcca tttaaccaaa tccaaaggtc
    16081 gcgtcacgga ggctgatatt catatcgcca gccagttgat ggaccgaatg aaccttcatg
    16141 gcgcttcccg tactgcggcg caaaatgcgt tccgggtggg aaaatcagat aattacccgc
    16201 tgcgcgaaaa gatgcgccag tttcgcagtg tctgctttgg tcgttttgac ttaattcgta
    16261 tgtttctgga gatccagatt caggcggcgt ttgctgatgg ttcactgcac ccgaatgaac
    16321 gggcggtgct gtatgtcatt gccgaagagt taggcatctc ccgcgcccag tttgaccagt
    16381 ttttgcgcat gatgcagggc ggtgcacagt ttggcggcgg ttatcagcag caatctggca
    16441 gtggtaactg gcagcaagcg cagcgtggcc cgacgctgga agatgcctgt aatgtgctgg
    16501 gcgtgaagcc gacggatgat gcgaccacca tcaaacgtgc ctaccgtaag ctgatgagtg
    16561 aacaccatcc cgataagttg gtggcgaaag gtttgccgcc tgagatgatg gagatggcga
    16621 agcaaaaagc gcaggaaatt cagcaggcct atgagctgat aaagcagcag aaagggttta
    16681 aataagtttc aacgtgtagg cctgataaga cgcggtaggc cggataagac gcgccagcgt
    16741 cgcatccggc gagcagcatc agacaatcgg cgcacaattg ccggatgtgg cgtgaacgcc
    16801 ttatccggcc taccgaactt cgccagcgtg actattaaaa atccgctggt gctttaaacg
    16861 tcatactatt gccatacgcc ggatgggtaa tcgtcagcat ctctgcatgt agcaacaaac
    16921 gcggtgccat tgctctcgct tctggtgatg cataaaaacg atcgccgaga atcggatgac
    16981 ccagcgccag catatgcaca cgcagctgat gcgaacgccc ggtaatcggt tttaacacca
    17041 ctcttgccgt gttatccgcc gcatattcca ccacttcata ctccgtctgc gcaggtttac
    17101 ctgtttcgta acagactttc tgtttcgggc ggtttggcca gtcgcaaatc agcggcagat
    17161 ccaccagacc ttccgcaggg gatggatgcc cccagacgcg ggccacatac tgttttttcg
    17221 gctcgcgctc gcggaactgg cgttttaact cccgctccgc ggctttggtc agcgccacta
    17281 caatcacgcc gctggtagcc atatccagac gatgcaccga ttctgcctgc ggataatcac
    17341 gctgaatgcg cgtcatcacg ctgtctttgt gctcttccag acgacccggc acagacaaca
    17401 aaccgctcgg cttgttgacc accataatat ggtcatcctg atacaggaca accagccagg
    17461 gttcctgcgg cggattgtag ttttccatcc ccatcttcgg ctccgttact gatgcgtcac
    17521 aacgatcaaa cgcagggcat ccagacgcca gcctgcctga tccaggcttt ccattacctg
    17581 ctgacggttg ctctcaatgg cggtcagttc gtcgtcacga atgttcgggt taactgcacg
    17641 cagcgcttcc agacgagaca gctcggcaga cagtttttcg tcggcttcgt tacgcgctgc
    17701 atcaatcaat gcacgggcag atttctcgat ctgcgcttcc cccagctgaa ggatagcgtg
    17761 aacatcctgc tgcaccgcgt taaccagctt gctgccggtg tgacggttaa ccgcgttaag
    17821 ctgacggtta aaggtttcaa attctacctg cgccgccagg ttgttgccgt ttttatccag
    17881 cagcatacgt accggcgtcg gtggcaggaa gcggttgagc tgcaactgct tcggagcctg
    17941 ggcttcaacc acataaatca gttccaccaa cagcgtacct accggcaacg ctttgttttt
    18001 caacagtgaa atcgtgctgc taccggtatc gccagaaagg atcagatcca gaccgttgcg
    18061 gatcagcgga tgctcccagg taataaactg tgcatcttca cgcgccagcg ccacttcacg
    18121 gtcgaaggtg atggtgatgc catcttccga caggccaggg aagtccggca ccagcatatg
    18181 atcggacggc gtcagcacga tcatgttgtc gccgcgatcg tcctgattga taccgataat
    18241 atcgaacagg ttcatggcga aggcgatcag attggtatcg tcatcctgct cttcaatgct
    18301 ttctgccagt gcctgggctt tttcgccacc gttggaatgg atctccagca ggcggtcacg
    18361 cccctgttcc agttgtgctt tcagcgcttc gtgctgctcg cggcagtttt tgattagatc
    18421 gtcaaagcct tcggtctcat ccggtgaagc cagatagtta atcagatcgt tgtatacgct
    18481 atcgtaaata gtgcgtccgg tcgggcaggt gtgctcgaac gcatccagac cttcgtgata
    18541 ccagcgcacc agcaccgact gagccgtttt ttccagataa ggcacatgga tctgaatatc
    18601 gtgcgcctga ccgatacgat ccagacgccc aatacgctgc tccagcagat ccgggttgaa
    18661 tggcaggtca aacatcacca tgtggctggc gaactggaag ttacgtcctt cagaaccgat
    18721 ttccgagcac agcagtacct gcgcaccggt gtcttcttca gcaaaccagg cggcagcgcg
    18781 gtcacgttcg ataatcgaca tgccttcgtg gaacactgcg gcgcgaatac cttcacgttc
    18841 gcgcagtacc tgctccagtt gcagcgcagt ggccgctttg gcgcagatca ccagcacttt
    18901 ctgagagcga tgactggtca ggtagcccat cagccattca acgcgcggat cgaagttcca
    18961 ccaggtggcg ttatcgcctt caaattcctg ataaatacgc tccggataga gcatatcgcg
    19021 agcgcgatct tccgcacttt tgcgtgcgcc cataatgccg gagactttaa tagcggtctg
    19081 atactgcgtc ggcagtggca gcttaatagt atgcagctcg cgtttcggga agcctttcac
    19141 accgttacgg gtgttacgga acagcacgcg gctggtaccg tggcgatcca tcagcatcga
    19201 aaccagttcc tgacgggcgc tctgggcatc ttcgctgtcg ctgtttgctg cttgcaacag
    19261 cggctcgata tcctgctcgc cgatcatctc accgagcatg ttcagttcgt cattgctgag
    19321 tttgttaccc gccagcagca tggcaacggc gtccgcaacc ggacgataat ttttctgctc
    19381 ttcaacgaat tgcgcaaaat cgtggaaacg gttcgggtcc agcagacgca gacgggcgaa
    19441 gtggctttcc atccccagct gttccggggt tgcggtcagc agcagaacgc ccggcacgtg
    19501 ctctgccagt tgttcaatgg cctgatattc acggctcggc gcatcttcgc tccacaccag
    19561 gtgatgcgct tcatcgacca ccagtagatc ccactcggct tcacagagat gttccagacg
    19621 ctgtttgcta cgacgggcaa aatccagcga gcaaatcacc agctgctcgg tatcgaacgg
    19681 gttgtaagca tcgtgctgag cttcggcata acgctcatca tcaaacagag caaagcgcag
    19741 gttgaaacgg cgcagcattt ctaccagcca ctgatgctgt aaggtttccg ggacgataat
    19801 cagcacacgt tcagcagcgc cagagagcag ttgctgatgc aggatcatcc cggcctcaat
    19861 ggttttccct aaacccactt cgtcagccaa caggacgcgc ggcgcgtggc ggcgaccaac
    19921 atcatgagcg atgttgagct ggtgcgggat caggctggta cgctgaccgc gcaggccgct
    19981 gtacggcata cggaactgtt cgctggaata cttacgcgcg cgataacgca gcgcaaagcg
    20041 gtccatacgg tcaatctgcc cggcaaacag acggtcctgc ggtttgctga acaccagttt
    20101 gctatcaagg aaaacttcac gcagggccac gccggactct tcagtatcaa ggcgagtacc
    20161 gatataggtc agcaagccat tttcttcttt tacttcttcg acttgcatct gccagccgtc
    20221 atggctggta atggtatcac cagggttgaa catcacgcgg gtcacggggg aatcactgcg
    20281 tgcgtacaga cggttttcac cagtagatgg gaaaagtaaa gtgacagttc gcgcatccac
    20341 cgcgacaacg gttccaagtc ccaattcgct ttctgtatcg ctgatccagc gttgaccaag
    20401 tgtaaaaggc atatgtgttc ggctctatat ctttaattgc aggcaataac cacccgctac
    20461 cgtgcttatg aggtagtggt gttattcagg tccaggaatg gaaagggcgc tatggtactg
    20521 gatggcaaag cattcgtcac gcatcaaaat ggtatctggc gaactctttt ttttgctcaa
    20581 aatagcccaa gttgcccggt cataagtgta gcaaaattat cctcaataaa agggagtatt
    20641 ccctccgcta cgggttgtag ctggcgggtc agatagtgtt cgtaatccag tggtgaacgt
    20701 tggtagtcca gcggctccgg gccgttggtg gtccatacgt acttaatggt gccgcggttc
    20761 tgatattgca aggggcgacc gcgcttatgg ttttcttcat cggcaaggcg agcggcgcgt
    20821 acatgaggcg gcacattacg ctgatattcg ctcagcggac ggcgaaggcg tttacggtaa
    20881 accagccgcg catccagttc acccgccatc agtttgtcga tggtttcacg cacgtattcc
    20941 tgatatggct cgttgcggaa gatacgcagg taaagctcct gctgaaactg ctgggccagt
    21001 ggcgtccagt cggtgcgcac ggtttccagc cctttaaaca ccatccgctg tttgtcgccc
    21061 tcctgaatca gtccggcgta gcgctttttg ctgccggtat cggccccgcg aatggttggc
    21121 atcagaaaac ggcagaaatg ggtttcatac tccagttcca gtgcgctggt cagccgttgt
    21181 ttttgcagcg tttccgccca ccaggcgtta acgtgctgca ccagtgcacg accgattttc
    21241 gctgcttctt cttccgaatg tgcacctttc agccagacaa acgttgagtc ggtatcgccg
    21301 tagataacgt catagccctg cgcttcaatc aacgttttgg tttgccgcat gatctgatga
    21361 ccacgcatgg tgatcgacga tgccaggcgt ggatcgaaga aacggcaggc ggtggtgccg
    21421 agcacaccat aaaaggcatt cataatgatc ttcagcgcct gcgaaagcgg cttattaccc
    21481 tgacgtttgg cttcatcgcg cccgtgccag atgttagtca caatctccgg caggcaatgt
    21541 ttctcccgtg agaaccacgc atcgagaaaa ccttcggtac tgtgctctgg atcaggctgc
    21601 gccatgcctt ccaccagccc gacgggatca atcagaaagg tgcggatgat cgacgggtac
    21661 aggcttttgt agtccagcac cagcaccgaa tcataaagcc caggtcgcga atccatcacg
    21721 tagccgccag ggctggcgtg cggcggcact tcgccgagat taggcgcgac ataaccagcg
    21781 cgatgcatac ggggaaaata gagatgacca aacgctgcca ccgaaccgcc gtgtcgatcc
    21841 accggcaggc cgttcaccgt cgcccgttcg agcaaaaacg gcataatttc agttttgtgg
    21901 aagatctgcg tcaccagctc gcaatctttc aggttataag ttgccagcgc aggtttatct
    21961 tcggcgaaac ggcggtcaat ttcgtccatt cgatcccacg ggttatcgat agattttcct
    22021 tcgcctaata actcctgagc gacggtttcc agcgagaatg aagagaaatt ccagaacgcg
    22081 gatttcagcg cctcgatacc gtcgataatt agccgacctt taacctgagc aaaaaacaca
    22141 ccgtttttaa agccgtgctc gcgccactcc agctcactgt tatcacgccc cagccgcagc
    22201 ggaatacggt aacgctcggc atgtttttgc agcattcgca gatcgaactg caccacgttc
    22261 caaccgatga tcacatcagg atcgtagttg gcaaaccagg cgttaagttt ttccagtagc
    22321 agcgggcggc tggcgacgta ttccagttca aaatcgagcg cggaggcgtc gccattctct
    22381 ggccccagca tataaacgat acgctgcccg caaccttcca ggccgatgca gtacagctca
    22441 ccgtggcggg tggtttcaat atcaatagaa acccacttga gcggcggacg atagtcggga
    22501 tgcggtttca gacgggcatt aacgatagcg ccattgcgca tatcaccctc gacccacacc
    22561 ggtgaggtga taaatcgctc catcagatag cgttctggcg ggcgcacatc ggcctcgtag
    22621 acggtaacgc caccttcacg caggcgcttt tcgtaattca tcaactggcg atgggcgcga
    22681 cagtaaaggc catacaccgg ctggcggtga aaatctttta acgccagcgg cgtcaggcga
    22741 aagccttgtt caccctgcaa aatatgctga gcgcggggaa cctgatcggc aggaataaac
    22801 gccacggact cttgcggcgc aaggaaaacc tgcaacggcc cgttgtccgt cgccagccag
    22861 aaggaaactt cggtcccttg cggggtgtcc cgccagtgtc gggttaagat aaaacctgcc
    22921 tgcgccacgc tgaaaatcca ataaaaaacc aggcttgatt atagcctggt ttcgtttgat
    22981 tggctgtggt tttatacagt cattactgcc cgtaatatgc cttcgcgcca tgcttacgca
    23041 gatagtgttt atccagcagc gtttgctgca tatccggtaa ctgcggcgct aactgacggc
    23101 agaatatacc catataagcg acctcttcca gcacgatggc attatgcacc gcatcttcgg
    23161 catttttgcc ccatgcaaat gggccgtgag aatggaccag gacgccgggc atttgcgctg
    23221 catcgatacc ctgtttttcg aaggtttcta cgatgacgtt accggtttcc cactcatatt
    23281 caccgttgat ttctgcgtcg gtcattttgc gggtgcaggg aatgggaccg tagaaatagt
    23341 cggcgtgggt ggtaccggtt gccggaattg actgacccgc ctgcgcccag atggtggcgt
    23401 ggcgcgagtg tgtgtgcaca atgccgccaa tggacgggaa tgcctgatag agcagccggt
    23461 gagttggcgt gtcggaggag ggctttttcg taccttcaac cacttcaccg gtttcgatgc
    23521 tgaccacgac catatcgtca gcggtcatga tgctgtagtc gacgccggaa ggtttgatca
    23581 caaagacgcc gcgcccgcga tcaacggcgc tgacgttgcc ccaggtgagc gtgaccaggt
    23641 tgtgttttgg cagcgccagg ttggcttcta atacctggcg tttgagatct tctaacatgt
    23701 tgactccttc gtgccggatg cgatttgctt atccggccta caaaatcgca gcgtgtaggc
    23761 ctgataagac gcgtcggcgt cgcatcaggc gttacatacc tgatgcggct acttagcgac
    23821 gaaatccgta atacacttcg ttccagcgca gcgcgtcttt aaacgctgga aggcgggtat
    23881 cgttatcaat caccgtgatt tcaatgtcgt gcatctcggc gaactggcgc atatcgttga
    23941 ggttcagcgc atggctgaag acggtatggt gcgcgccacc ggcaaggatc cacgcttcgg
    24001 aagcggtcgg cagatccggt tgcgctttcc acagcgcatt cgccaccggc agtttcggca
    24061 gggagtgcgg tgttttcacc gtgtcgatgc agttaaccag cagacgataa cgatcgccga
    24121 gatcaatcag gctggcgaca atggccggac cagtttgagt attgaagatc aggcgagccg
    24181 ggtcgtcttt accgccaata ccaaggtgct gaacgtcgag gatcggtttc tcttccacag
    24241 cgatcgacgg gcagacttcc agcatatggg agccgagtac caggtcatta cctttctcga
    24301 agtgataggt gtagtcctcc ataaaggagg tgccgccctg cagaccggtt gacatcacct
    24361 tcatgatgcg aagcagggcg gcagttttcc agtcgccttc gcccgcaaag ccgtagccct
    24421 gctgcatcag acgctgtacg gccagaccag gaagctgttt caggccgtgc aaatcttcaa
    24481 aggtagtagt gaaagcgtgg aagccaccct gttccaggaa acgcttcatc cccagctcaa
    24541 tacgcgccgc ttccagcacg ttctgtcgtt tttcgccgtg gatttgtgtg gcaggtgtca
    24601 tggtgtagca gctttcgtac tcatcgacca gcgcgttaac atcgccgtcg ctgatggagt
    24661 tcaccacctg caccagatcg ccaaccgccc aggtattgac ggagaaacca aacttgatct
    24721 gtgcggcaac tttatcgcca tcggtgaccg ctacttcacg catgttatcg ccaaaacggc
    24781 agactttcag atgacgggta tcctgtttag agaccgcctg acgcatccag gagccgatac
    24841 gctcatgtgc ttgtttatcc tgccagtgac cggtaacaac ggcatgttgc tgacgcatac
    24901 gcgcgccgat gaagccgaac tcgcgaccgc catgcgcggt ctggttcagg ttcataaagt
    24961 ccatgtcgat gctgtcccac ggcagcgccg cgttgaactg ggtgtggaat tgcagcaacg
    25021 gtttgttgag catggtcagg ccgttgatcc acattttggc cggggagaaa gtgtgcagcc
    25081 acaccaccag accagcgcaa cgatcgtcgt aattcgcgtc gcggcaaata gcggtgattt
    25141 catccggcgt ggtgcccagc ggtttcagca ccagtttgca gggcaacttc gcttctgtat
    25201 tcagtgcatt aacaacgtgt tccgcatgtt gcgtcacctg gcgcagagtc tccgggccgt
    25261 aaagatgctg gctgccaatt acaaaccaca cttcataatt atcaaaaatc gtcattgtcg
    25321 tgtccttata gagtcggaac ggcctgggca gcctgtgccg gggcggaagt tggaagatag
    25381 tgttgttcgg cgctcatcgc ccattgctga tagcggcgat aaagctgttc aaagcgttgt
    25441 gcctgttcgc tgcgcggttg cagggttttc tctaccgcac tggccatttt ttgctgggct
    25501 gatgggatgt ctgcgtgcac tttcgcggcg acagcagcaa aaatcgccgc accgagcgca
    25561 cagcactggt cagaggcaac aatttgcagc gggcgattca gcacgtcgca gcaggcctgc
    25621 ataatgacct ggtttttccg cgcgatgccg cccagcgcca tcacgttatt gacggcgatc
    25681 ccctgatcgg taaagcactc catgattgcg cgtgcgccaa aggcggtggc agcaatcaaa
    25741 ccgccgaaca gcagcggagc gtcggtagcg aggttcagat cggtaatcac ccctttcagg
    25801 cgttggttag cgttcggtgt gcggcggccg ttaaaccagt cgagcaccac cggtaggtga
    25861 tccagagacg gattttgagc ccatacttcg gtcagcgccg gaagcagttg tttctggctg
    25921 gcgttgattt gctctttcag ttccggatgc tgggcggcaa gctgttccag cggccagccg
    25981 agtacgcgac caaaccaggc gtagatatcc ccaaacgccg attggcctgc ttccagaccg
    26041 ataaatccag gcaccacgct gccatcaacc tgaccgcaaa tacctttcac tgcccgctcg
    26101 ccaacgctct gtttgtcggc aatcagaatg tcacaggtgg aagtaccgat aacttttacc
    26161 agtgcgttag gctgtgcgcc tgcgccaact gcgcccatat ggcagtcaaa cgcgccgccg
    26221 gaaatcacta cgctttcagg caggccgaga cgctgcgccc attccgggca taaggtgccc
    26281 accggaatat cggcagtcca ggtatcagtg aacagcgggg aaggcaaatg gcgattgagg
    26341 atcgggtcca gctcatcaaa gaaactggct ggcggcaggc caccccagct ttcgtgccac
    26401 agagatttat gcccggcgct gcaacgtccg cgacgaatat cctgcgggcg ggtggtaccg
    26461 gaaagcagag ctggcaccca gtcgcacagc tcaatccatg atgcggcaga ttgtgccacg
    26521 gcgttgtcct ggcgagttac atgcaggatt tttgcccaga accattcgct ggaataaata
    26581 ccgccaatat tgcgggagta gtcaacgttg cccggcgtgt gacataagcg ggtaatctct
    26641 tccgcttctt caaccgcggt gtggtctttc cacaatacga acatcgcgtt cgggttctcg
    26701 gcaaactccg ggcgcagcgc caacacgttg ccgtcggcgt caatcggtgc gggcgtcgag
    26761 ccggttgtgt caacgccaat cccgaccaca gctgcgcgct gttcgacgca aagctctgca
    26821 agcacagttt tcagcgccgc ttccattgac tcaatgtagt cacgcggatg atgacggaac
    26881 tggttattcg gggcatcaca aaattgcccg ttctgccaac ggggatacca ctctacgctg
    26941 gtggcgatct cttcaccagt ggcgcattcc accgccaaag ctcgcacaga atcactgcca
    27001 aaatcgaggc caattgcaat cgccatcgtt tcactccatc caaaaaaacg ggtatggaga
    27061 aacagtagag agttgcgaaa aaaaacgtca ggctggatcc gctaatctta tggataaaaa
    27121 tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg
    27181 attatagaca cttttattac gcgtttttgt catggctttg gtcccgcttt gttacagaat
    27241 gcttttaata agcggggtta ccggttgggt cagcgagaag agccagtaaa agacgcagtg
    27301 acggcaatgt ctgatgcaat atggacaatt ggtttcttct ctgaatggcg ggagtatgaa
    27361 aagtatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta acgcccatct
    27421 ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta tcgaccgacc
    27481 gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcagggca tagtgaaaaa
    27541 ccagggacga gaatttgtct gccgaccggg tgatattttg ctgttcccgc caggagagat
    27601 tcatcactac ggtcgtcatc cggaggctca cgaatggtat caccagtggg tttactttcg
    27661 tccgcgcgcc tactggcatg aatgggttaa ctggccgtcg atatttgcca ataccggttt
    27721 ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa
    27781 tgccggacaa ggggaagggc gctattcgga attgctggcg ataaatctgc ttgagcaatt
    27841 gttactgcgg cgcatggaag cgattaacga gtcgctccat ccgccgatgg ataatcgggt
    27901 acgcgaggct tgtcagtaca tcagcgatca cctggcagac agtaattttg atatcgccag
    27961 cgtcgcacag catgtctgcc tgtcgccgtc gcgtctgtcg catttgttcc gccagcagtt
    28021 agggattagc gtattaagct ggcgcgagga ccagcgcatc agtcaggcga agctgctttt
    28081 gagcaccacc cggatgccta tcgccacagt cggtcgcaat gtcggttttg acgatcaact
    28141 ctatttctcg cgagtattta aaaaatgcac cggggccagc ccgagcgagt ttagggctgg
    28201 atgtgaataa taagaaagaa gggggagtct cttactttcg
//
LOCUS       DAJOTB010000052        28207 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJOTB010000052 DAJOTB010000000
VERSION     DAJOTB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.052"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="OHN28_002183"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="OHN28_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2297580.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(370..2484)
                     /gene="fusA"
                     /locus_tag="OHN28_002184"
     CDS             complement(370..2484)
                     /gene="fusA"
                     /locus_tag="OHN28_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCQ2297581.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="OHN28_002185"
     CDS             complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="OHN28_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCQ2297582.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="OHN28_002186"
     CDS             complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="OHN28_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCQ2297583.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="OHN28_002187"
     CDS             complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="OHN28_002187"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCQ2297584.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="OHN28_002188"
     CDS             complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="OHN28_002188"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCQ2297585.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="OHN28_002189"
     CDS             complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="OHN28_002189"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCQ2297586.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4687..5409)
                     /locus_tag="OHN28_002190"
     CDS             complement(4687..5409)
                     /locus_tag="OHN28_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2297587.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="OHN28_002191"
     CDS             complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="OHN28_002191"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2297588.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6609..6827
                     /locus_tag="OHN28_002192"
     CDS             6609..6827
                     /locus_tag="OHN28_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCQ2297589.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="OHN28_002193"
     CDS             complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="OHN28_002193"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2297590.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7561..7761)
                     /locus_tag="OHN28_002194"
     CDS             complement(7561..7761)
                     /locus_tag="OHN28_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCQ2297591.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="OHN28_002195"
     CDS             complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="OHN28_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCQ2297592.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="OHN28_002196"
     CDS             complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="OHN28_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCQ2297593.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10258..12171
                     /locus_tag="OHN28_002197"
     CDS             10258..12171
                     /locus_tag="OHN28_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2297594.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12171..13193
                     /locus_tag="OHN28_002198"
     CDS             12171..13193
                     /locus_tag="OHN28_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2297595.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13187..13405
                     /locus_tag="OHN28_002199"
     CDS             13187..13405
                     /locus_tag="OHN28_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCQ2297596.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13459..14328
                     /locus_tag="OHN28_002200"
     CDS             13459..14328
                     /locus_tag="OHN28_002200"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCQ2297597.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14383..14787)
                     /locus_tag="OHN28_002201"
     CDS             complement(14383..14787)
                     /locus_tag="OHN28_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCQ2297598.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15089..15721
                     /gene="crp"
                     /locus_tag="OHN28_002202"
     CDS             15089..15721
                     /gene="crp"
                     /locus_tag="OHN28_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCQ2297599.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15772..17862
                     /locus_tag="OHN28_002203"
     CDS             15772..17862
                     /locus_tag="OHN28_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2297600.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="OHN28_002204"
     CDS             complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="OHN28_002204"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCQ2297601.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="OHN28_002205"
     CDS             complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="OHN28_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCQ2297602.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19830..20432)
                     /locus_tag="OHN28_002206"
     CDS             complement(19830..20432)
                     /locus_tag="OHN28_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCQ2297603.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(20422..20589)
                     /locus_tag="OHN28_002207"
     CDS             complement(20422..20589)
                     /locus_tag="OHN28_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCQ2297604.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="OHN28_002208"
                     /gene_synonym="rotA"
     CDS             complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="OHN28_002208"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCQ2297605.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21537..22718
                     /gene="tsgA"
                     /locus_tag="OHN28_002209"
     CDS             21537..22718
                     /gene="tsgA"
                     /locus_tag="OHN28_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCQ2297606.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22980..25523
                     /gene="nirB"
                     /locus_tag="OHN28_002210"
     CDS             22980..25523
                     /gene="nirB"
                     /locus_tag="OHN28_002210"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCQ2297607.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25520..25846
                     /gene="nirD"
                     /locus_tag="OHN28_002211"
     CDS             25520..25846
                     /gene="nirD"
                     /locus_tag="OHN28_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCQ2297608.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25973..26779
                     /gene="nirC"
                     /locus_tag="OHN28_002212"
     CDS             25973..26779
                     /gene="nirC"
                     /locus_tag="OHN28_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCQ2297609.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26798..28171
                     /gene="cobA"
                     /locus_tag="OHN28_002213"
     CDS             26798..28171
                     /gene="cobA"
                     /locus_tag="OHN28_002213"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2297610.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6812 a         7554 c         7140 g         6701 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg
      181 taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg
      241 tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg
      301 gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa
      361 cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc
      421 atcatacttc aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg
      481 agttgcatat ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac
      541 ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc
      601 ggtgttctct tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt
      661 cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga
      721 ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta
      781 gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta
      841 ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga
      901 acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga
      961 ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc
     1021 aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat
     1081 gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt
     1141 ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc
     1201 ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat
     1261 acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt
     1321 cagaccgatt gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt
     1381 agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac
     1441 ggtatcacca gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa
     1501 cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg
     1561 ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg
     1621 ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc
     1681 agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt
     1741 gatttctgct tcagtcagtt cttcaccacc cagatatttt tccatcagct cttcagaagc
     1801 ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc
     1861 cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt
     1921 cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag
     1981 cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc
     2041 acccatgcgg tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg
     2101 acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc
     2161 accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt
     2221 gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt
     2281 agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt
     2341 tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg
     2401 ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg
     2461 tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa
     2521 gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga
     2581 ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg
     2641 tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag
     2701 agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc
     2761 attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg
     2821 agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc
     2881 agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt
     2941 agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa
     3001 cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     3061 ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     3121 cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     3181 acgagcctgc ttacggtctt taacaccgga gcagtcaagc gcaccacgta cggtgtggta
     3241 acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     3301 caagttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     3361 acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     3421 acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     3481 gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt
     3541 agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt
     3601 tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc
     3661 tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg
     3721 ttcgaaattc gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta
     3781 atgggggcat tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca
     3841 tcttgcaata atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc
     3901 gtcagccagg gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca
     3961 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca
     4021 aagggtgtct gcggatctaa cccacgttcg cgcagtgaag ccgcgcaaat ccagcactgt
     4081 tcaatatcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4141 tcgggctttt gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4201 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccggcc tgcagttgta
     4261 ccatgaggcg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4321 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4381 ggtttgacga agcaagcccc agtcttccgg cctccgtttc atcaacaacg ccacggcgta
     4441 atgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4501 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4561 cttcccgata gaaaaaaacg ctgcttaact catggccttc tgctatcagc gcctgcgcaa
     4621 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4681 aacgcattac ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata
     4741 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4801 gaagatccct ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac
     4861 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4921 aacagaagag gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt
     4981 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5041 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5101 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5161 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5221 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5281 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5341 ctggtcaaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5401 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5461 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5521 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5581 ttttagcaga gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5641 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
     5701 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5761 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
     5821 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5881 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     5941 aaaccagacc agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6001 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6061 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct
     6121 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6181 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6241 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6301 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6361 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6421 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6481 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6541 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6601 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6661 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6721 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6781 cgaacatcgc gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag
     6841 cgggaattcc ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg
     6901 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca
     6961 ccgcagcaac cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca
     7021 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7081 ttctgaccag ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7141 atttcaaccg gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt
     7201 tcatcaacgc ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga
     7261 ccgtaagcgt cattcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7321 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7381 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7441 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7501 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7561 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc
     7621 tttgtctgct tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt
     7681 ttcgcgccac atcgccattg aatcctgagc ctgacaggcc gggcattttg cgcccgcaat
     7741 aaaacgtttt cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg
     7801 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7861 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7921 atccaggcgg cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt
     7981 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8041 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg
     8101 caaatgcgga aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt
     8161 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg
     8221 cgtggcgtcg ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat
     8281 atcccgctcc agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8341 accaaaacgc ccaaagccca cgacaataac ctgcggttta tcatcgttga cccatggttt
     8401 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcta ccagcttcat
     8461 cagcagcggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8521 gccctggaat aagcgttgtg aagaagcgga agaaaagagg acaaaggcaa actcaccgcc
     8581 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8641 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca
     8701 caacagatgg gtataaagca ccccgaggtt gagtgacatg ccgacagaga taaagaacaa
     8761 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8821 ttccgccagt agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc
     8881 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     8941 accagaagct gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat
     9001 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9061 acccgccaac aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag
     9121 taccgagaaa cctaactggc cggattcgct gcgattcatc cctttttcac gcatcaactg
     9181 caatgccatt gcggttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9241 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9301 cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag
     9361 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9421 gtcgctaata aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa
     9481 cactgcacca atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa
     9541 cagcactcct gcgagtaaaa aatcagaacc ttccatcagc ggcctcctgg agacaacgga
     9601 tttgccagcc agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt
     9661 ctcgcccagt aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt
     9721 tcaaaggggc gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca
     9781 ctttccggct ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc
     9841 cccggtccgc tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc
     9901 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
     9961 gcctgctcac gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg
    10021 gtaacattgc tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga
    10081 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10141 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10201 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10261 attgtttttt cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10321 gccaccatca accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10381 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10441 ggaagctggc aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg
    10501 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc
    10561 aacgaacgta acgacgggca agccattgca accattcatg gcaagctgga tgctattgac
    10621 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10681 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10741 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10801 ctcgatgccg ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg
    10861 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa
    10921 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    10981 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtagcgca tctgcaaagt
    11041 tatatcgacc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgtattaag
    11101 atgctggagc gtatggagct gattgccccg gcgcacgtcg acaacccgtt ccgctttagc
    11161 ttccgcgcgc cggaaagcct gcctaatccg ttattaaaga tggaaaaagt cagcgcaggc
    11221 tatggtgatc gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt
    11281 ggtctgctag gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11341 cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc
    11401 cagcatcaac ttgaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta
    11461 gcgccgcagg agctggaaca aaaactgcgt gattacctcg gcggctttgg tttccagggc
    11521 gataaagtaa ccgaagaaac gcgccgcttt tccggtgggg aaaaagcccg cctggtgctg
    11581 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11641 gacctcgaca tgcgtcaggc actcactgaa gcattaatcg agttcgaagg cgcgctggtt
    11701 gtcgtctcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11761 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11821 gtacaaaagc aggaaaacca ggccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11881 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt
    11941 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12001 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12061 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12121 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12181 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12241 ttagcaattg tcatctgcaa accatgctgc cgcgtctgtt tcgtcgcaag gtgaaattca
    12301 ccccgcactg gcatcgactg gagttgcccg acggcgattt tgtcgatctc gcatggagtg
    12361 aagatcctgc acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca
    12421 gtctcaatag cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12481 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12541 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc
    12601 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12661 caaaagaagg taatgatctc ccgattgatg cggcggtgat tgtctctgcg ccgtttatgc
    12721 tggaggcctg tagttaccat atggaaaagg gcttttcccg catttatcag cgttacttgc
    12781 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga
    12841 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg
    12901 ccagaattca cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga
    12961 tgctcaatcg gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg
    13021 atcatcaggt gatcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg
    13081 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13141 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13201 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13261 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13321 gctacagtgc ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat
    13381 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag
    13441 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13501 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13561 catgcagccg aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg
    13621 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13681 gacttcggcc ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct
    13741 cgcaaatatc tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc
    13801 accccctggc aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc
    13861 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg
    13921 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    13981 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14041 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca
    14101 aacccgttcg cggcaaaagg tatcccgtca ctggatgaaa gctttgtggt gatccatttt
    14161 cgtaatctgg aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt
    14221 tcccacatca atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt
    14281 atgctgccgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac
    14341 gccggataaa atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc
    14401 atacgagtga gtaatattca ccgctttttc cagcatcagc gccactgagc aatatttctc
    14461 ggcagaaaga tcaaccgcac gcgcaaccgc tgcgtctttc agatcgcgac cggtgacgat
    14521 aaaatgtaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa
    14581 ttttacttca caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat
    14641 ggcactgcaa ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc
    14701 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14761 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14821 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14881 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt
    14941 gatgtactgc atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt
    15001 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15061 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15121 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15181 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15241 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15301 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15361 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15421 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15481 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct
    15541 ggcaaaacaa ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15601 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15661 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15721 atcccgtcag agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15781 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15841 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt
    15901 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta
    15961 atcattggtg cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa
    16021 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16081 ctggggccat tgcacgcaaa attgcttccc gcatcgctgc tcgccgccat ttttaccctc
    16141 agtctggcgg gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg
    16201 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag
    16261 tcactaagtc tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt
    16321 acccagcaca ccgaccctga aaaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg
    16381 gtcgatctaa ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc
    16441 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16501 gtcagtttgc atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa
    16561 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16621 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16681 atcgcccgcc aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16741 attgctcgtg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16801 cagcggcgaa tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg
    16861 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg
    16921 ctgcatctgc cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc
    16981 tatggcgcaa cccgtctgag gattgtaaat cgctccgtag gaaccgtggt cgggttaatc
    17041 attgcaggcg tggcgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg
    17101 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt
    17161 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17221 cttccgcgtc ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg
    17281 ctgtggccgc agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc
    17341 tatcaggacg cgattcgctt gattctgagc gaggacccgc aacctactcc actggcctgg
    17401 cagcgaatgc gggtaaatca ggcgcataat actctgtata actcattgaa tcaggcgatg
    17461 caggaaccgg cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17521 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17581 ccaccggaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17641 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17701 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt
    17761 ggccatctga acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac
    17821 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17881 atgaggtgtt cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acgccccaac
    17941 caccttcgcc accgcgtggg cgaaacgatg catcccttca tcgatatccg catcttccac
    18001 caccagcgac ggcgcaaaac gcatcacatc aggcccggca ttcagcacca ttacgccttc
    18061 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tactgtggtt tcagctctgc
    18121 gccaatcaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18181 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaata ccttccagca cttcaggggt
    18241 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18301 ggaaccgtga gaaccaggat gaaacgcgga agcaatttcc gccgtggtca gcatggcgct
    18361 aatcgggaag ccgccgccta acgctttcgc agaggtcaga atatccggcg tcacgccgta
    18421 gtgcatgtaa gcaaacaaat cgccggtccg ccccatcccg cactgtactt catcaaacac
    18481 caataatgcc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18541 tgccgtcaca ccgccctcac cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18601 catcaccgct ttcactgcgt ggagatcgtt aaagggaacg tggatgatgt ctgccggttt
    18661 tggcccaaag ccatcggaat attttggttg cccgcccacc gagacggtaa acagcgagcg
    18721 accatgaaaa gcgttatgga aggcgataat tttggttttg aacgggctat gacgcacaca
    18781 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18841 caccacgcgt tcggcaaacg ttgcctcaat cagtttacga ccaagacgca gcgccggttc
    18901 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    18961 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19021 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19081 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19141 aattgccatc tcatgatcac cctgttacgc ataaaccaat gtgaaattat aatcacaaaa
    19201 tatgcataaa aaatcactta atagcaatca gaaatcagcg atgcaggaaa ttagccagca
    19261 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ttgcacacct tccagatccc
    19321 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acttcaaagc
    19381 actccggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtgagtggat
    19441 ttgccagccc cttaaatacg cccacaccat tatgtgtaat cggcgaggtt ttgccgtgca
    19501 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg tgccattgcc tggtgaccga
    19561 ggcagacgcc aagaatcggc aaacgcccgg catagtgacg aataacgtca agggagatcc
    19621 cggcttcatc tggcgtacag gggccgggtg agatgacaat tttttgtggt ttaagggcgt
    19681 cgatatccgc caacgtcaac gcatcgttgc gcttaaccaa cacatccgcc cccagttcac
    19741 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atcaataagc aggatcatag
    19801 cggctccggt acgaaagaac cgcgctattt tactcagatt ccccgacttc gcttaccact
    19861 ttgctaaata tcatctgcag cgcggtgaga tcccccattg ccccattctg atttgcctga
    19921 ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    19981 tgctcgaaga agatccgctg tgccaaacca cttcccaccc ggaagggatg cagcacgttg
    20041 atttcacaat agtaatgcgc caaccgctcg acgaatttat ctttctcaag gctagtcaga
    20101 tacttctctt cctccaaatc ctgcatcagg gcattgccct ctttttcgag ataggcaaag
    20161 tggcagaacg gcgtatctcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20221 aaaatatcct gatacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20281 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20341 cgctgctgct ggcggatgtt gagcttgtta cgcatgatat cgagacccgg gtagagatat
    20401 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagcgctt caaggcgcgc
    20461 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20521 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20581 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20641 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20701 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaacg
    20761 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20821 aataccgcgt aaccgaaatc acgctgaccg tggtcaagga aggcgttatc ggcaacgttg
    20881 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    20941 cgcgtgttgc gcaggccgtt atcagcttca tttttgattg gcgggtttgg ttttttctgc
    21001 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21061 gtcgtgttgt tataaaaacc actgttcaca taatcgacaa agttttgcac agacactggc
    21121 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21181 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21241 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21301 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21361 atctgaaata tatgtaacct gaggataact ttttattccg tgatcaaaat cacctcttaa
    21421 aatgcaattt agcaaccgat tgcaataaaa catttaatca gatcacaaaa tcgcctaaaa
    21481 tcgcccgctc gcggcatagc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21541 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ttcctacgca ctgaccggtg
    21601 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21661 tttccagcat gagtaacacc ttcaccttcc tcaatgccgg cattttaatc tctatcttcc
    21721 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21781 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcggcgg
    21841 cgatgttcat tctcggggtg gtcagcggca tcaccatgtc gattggtaca ttcctggtaa
    21901 cacaaatgta tgaaggacgt cagcgcggtt cacgcctgtt atttaccgac tccttcttca
    21961 gtatggccgg gatgattttc ccaatgatcg ccgcatttct gctggcgcgt agcattgagt
    22021 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    22081 gctgtgagtt cccggcgctg ggcaaacatg cgcccaaaac ggatgctccg gtagagaaag
    22141 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22201 agttaggttt tatctcctgg gtgccggaat atgccaaagg cctgggcatg agcctgaacg
    22261 acgcaggcac gctggtaagt aacttctgga tgtcgtacat ggtcggcatg tgggcattca
    22321 gctttattct tcgcttcttt gatttgcaac gtatcctgac cgtactggct ggtctggctg
    22381 cgattctgat gtacgtcttt aacaccggaa caccggcaca tatggcgtgg tcaattctcg
    22441 ccctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22501 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcgggacc atcggtacta
    22561 tgttgacctt tgtggttacc ggcccgattg ttgaacatag tggtccgcag gcggcgctgc
    22621 tgacggcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22681 gccgtcaccg tcagcataac accctgacct ctcattaatt gttcatgccg ggcggcacta
    22741 tcgtcgtccg gccttttcct ctcttaccct gctacgtgca tctatttcta taaacccgtt
    22801 caatttgtct gttttttgca caaacatgaa atatcagaca attccgtgac ttaagaaaat
    22861 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22921 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    22981 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23041 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23101 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23161 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23221 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23281 tttatgacaa gctgatcatg gcgaccggtt cctacccgtg gatcccgcca atcaaaggtt
    23341 ctgatactca ggactgcttt gtctatcgca ctattgaaga cctcaacgcc attgaatcct
    23401 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23461 ctggcgcgct gaaaaactta ggcattgaaa cccacgttat cgaatttgcc cctatgctga
    23521 tggcggaaca gcttgatcag atgggcggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23581 gcgtgcgcgt tcacaccagc aaaaacactc ttgagattgt gcaggaaggt gttgaagcgc
    23641 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23701 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23761 ggggtatcgt cataaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23821 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23881 aggtcgccgt tgaccatatt cttggtagcg aaaacgcctt tgaaggtgca gaccttagcg
    23941 ccaagctgaa actgctgggc gtagacgtag gcggtattgg tgatgcgcac ggtcgcacgc
    24001 ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga gatctacaaa cgcctgattg
    24061 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24121 gcaacctgct gcaactggtg ctgaacgcta tcgaactgcc ggaaaacccg gattctctga
    24181 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24241 gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga cctgattgct gctatcaaca
    24301 aaggctgcca caccgttgcg gcgctgaaag ccgaaaccaa agccggtacg ggctgcggcg
    24361 gttgtattcc gctggtcact caggtactga acgcggaact ggcgaaacag ggtatcgaag
    24421 ttaacaacaa cctgtgtgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24481 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggttacggtt
    24541 gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24601 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaaa
    24661 aagacggcac ctactcggtg atcccgcgtt ctccgggtgg tgaaatcacc ccggaagggc
    24721 tgatggcagt gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    24781 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggagatctgg cgtcagctga
    24841 ttgaagcagg tttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24901 gcgtgggtag cacctggtgc cgctatggcg ttggcgacag cgtcggcctc ggcgtggaac
    24961 tggaaaatcg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25021 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25081 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25141 cagcggatat cgatcgcgaa acgctgatta aatatcttga ccgcttcatg atgttctaca
    25201 tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggtggca
    25261 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgca catctggaag
    25321 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaataccc
    25381 cgtctgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25441 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25501 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25561 gcctgaaacc ggcgtctgcg cgctgttagg tgacgaacaa gtagcaattt tccgcccgta
    25621 tcacagcgac caggtgtttg cgatcagcaa catcgatccg ttcttcgagt ccagcgtgct
    25681 gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25741 gcagcgtttt cgcttaagcg acggcttgtg catggaagac gaacagtttt ccgtcaagca
    25801 ttacgacgcg cgagtgaaag acggcgtggt gcagctgcgc ggttaatatt ttaacgggag
    25861 gcgcaatgcc tccccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    25921 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    25981 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26041 cccgctcggc ttttgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26101 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggtgc
    26161 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26221 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26281 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtctttgttg ccatgctcta
    26341 tagctggggt ggcggtagcc tgctgccggt cgataccagc atcgttcact ccgtcgcgct
    26401 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26461 ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26521 ctggtggtgt ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26581 gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26641 tcataacctg ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt
    26701 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    26761 tgaaacggct gccggttaat tattaagggg tttttacgtg gatcatttgc ctatattttg
    26821 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    26881 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    26941 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27001 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    27061 cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27121 cgccagcttt attatgccgt cgattattga ccgctcgccg ctcatggtag cggtctcctc
    27181 tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc
    27241 gttacatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27301 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta atgaccgcct
    27361 ggcgcagtcg ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    27421 caatgaaccg ctcgatcatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    27481 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    27541 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    27601 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27661 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    27721 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc
    27781 gggtattacc gcagcctctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    27841 ttatgcccag agcgtacgct taattactgg acacttaaaa accggtggcg agctggactg
    27901 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    27961 aactattcag caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tagcaattgt
    28021 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28081 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28141 cgataaattg aactggttct ctaaccatta atttaaccag ggccagagaa gtctggcctt
    28201 tttcgtt
//
LOCUS       DAJOTB010000053        27577 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJOTB010000053 DAJOTB010000000
VERSION     DAJOTB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.053"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            217..720
                     /gene="dps"
                     /locus_tag="OHN28_002214"
                     /gene_synonym="pexB"
     CDS             217..720
                     /gene="dps"
                     /locus_tag="OHN28_002214"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCQ2297611.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1124..1870
                     /gene="glnH"
                     /locus_tag="OHN28_002215"
     CDS             1124..1870
                     /gene="glnH"
                     /locus_tag="OHN28_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCQ2297612.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2009..2668
                     /gene="glnP"
                     /locus_tag="OHN28_002216"
     CDS             2009..2668
                     /gene="glnP"
                     /locus_tag="OHN28_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCQ2297613.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2665..3387
                     /gene="glnQ"
                     /locus_tag="OHN28_002217"
     CDS             2665..3387
                     /gene="glnQ"
                     /locus_tag="OHN28_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCQ2297614.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3504..5729
                     /gene="ybiO"
                     /locus_tag="OHN28_002218"
     CDS             3504..5729
                     /gene="ybiO"
                     /locus_tag="OHN28_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCQ2297615.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5726..6652)
                     /gene="rlmF"
                     /locus_tag="OHN28_002219"
     CDS             complement(5726..6652)
                     /gene="rlmF"
                     /locus_tag="OHN28_002219"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCQ2297616.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6928..7188
                     /locus_tag="OHN28_002220"
     CDS             6928..7188
                     /locus_tag="OHN28_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2297617.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7453..9735
                     /locus_tag="OHN28_002221"
     CDS             7453..9735
                     /locus_tag="OHN28_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCQ2297618.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9777..10454
                     /gene="ybiX"
                     /locus_tag="OHN28_002222"
     CDS             9777..10454
                     /gene="ybiX"
                     /locus_tag="OHN28_002222"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCQ2297619.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10528..10794
                     /gene="ybiI"
                     /locus_tag="OHN28_002223"
     CDS             10528..10794
                     /gene="ybiI"
                     /locus_tag="OHN28_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCQ2297620.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            11059..11319
                     /locus_tag="OHN28_002224"
     CDS             11059..11319
                     /locus_tag="OHN28_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2297621.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11457..12542)
                     /gene="hcxB"
                     /locus_tag="OHN28_002225"
     CDS             complement(11457..12542)
                     /gene="hcxB"
                     /locus_tag="OHN28_002225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCQ2297622.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12682..13644)
                     /gene="ybiB"
                     /locus_tag="OHN28_002226"
     CDS             complement(12682..13644)
                     /gene="ybiB"
                     /locus_tag="OHN28_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCQ2297623.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(13672..15822)
                     /gene="dinG"
                     /locus_tag="OHN28_002227"
     CDS             complement(13672..15822)
                     /gene="dinG"
                     /locus_tag="OHN28_002227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCQ2297624.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15926..16126
                     /locus_tag="OHN28_002228"
                     /pseudo
     CDS             15926..16126
                     /locus_tag="OHN28_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16359..17720)
                     /gene="rhlE"
                     /locus_tag="OHN28_002229"
     CDS             complement(16359..17720)
                     /gene="rhlE"
                     /locus_tag="OHN28_002229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCQ2297625.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17949..18620
                     /gene="cecR"
                     /locus_tag="OHN28_002230"
     CDS             17949..18620
                     /gene="cecR"
                     /locus_tag="OHN28_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCQ2297626.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18623..19618
                     /gene="hlyD"
                     /locus_tag="OHN28_002231"
     CDS             18623..19618
                     /gene="hlyD"
                     /locus_tag="OHN28_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCQ2297627.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            19611..21347
                     /locus_tag="OHN28_002232"
     CDS             19611..21347
                     /locus_tag="OHN28_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2297628.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21340..22473
                     /locus_tag="OHN28_002233"
     CDS             21340..22473
                     /locus_tag="OHN28_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297629.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22484..23590
                     /locus_tag="OHN28_002234"
     CDS             22484..23590
                     /locus_tag="OHN28_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297630.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23552..23962)
                     /locus_tag="OHN28_002235"
     CDS             complement(23552..23962)
                     /locus_tag="OHN28_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCQ2297631.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24095..24856
                     /locus_tag="OHN28_002236"
     CDS             24095..24856
                     /locus_tag="OHN28_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2297632.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24853..26094
                     /gene="clsB"
                     /locus_tag="OHN28_002237"
     CDS             24853..26094
                     /gene="clsB"
                     /locus_tag="OHN28_002237"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCQ2297633.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26094..27050
                     /locus_tag="OHN28_002238"
     CDS             26094..27050
                     /locus_tag="OHN28_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCQ2297634.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27086..>27577)
                     /locus_tag="OHN28_002239"
     CDS             complement(27086..>27577)
                     /locus_tag="OHN28_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCQ2297635.1"
                     /translation="NKINTAIAGVIYLFLISLVAIVVASLAPVSAIIVVFSTAGSMFL
                     ISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERPIWVISCLMIVLWSGII
                     SHGRNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFGILAAIAITLVWQRHTR
                     FFH"
BASE COUNT         6514 a         7319 c         7195 g         6549 t
ORIGIN      
        1 gcaaaagcat atttattccg aaaattcctg gcgagcaaat aaataagaat tgttcttatt
       61 aatatatcta actcattgat ttttttattg tttttgtttt tcacgcttgt taccactatt
      121 agtgtgatag gaacagccag aatagcggaa cacatagctg gtgctatact tagtctcgtt
      181 aattactggg acataacatc aagaggatat gaaattatga gtaccgctaa attagttaaa
      241 tcaaaagcga ccaatctgct ttatacccgc aacgatgtct ctgacagcga gaaaaaagca
      301 acagtagagt tgctgaatcg ccaggttatc cagtttattg atctttcttt gattaccaaa
      361 caagcgcact ggaacatgcg cggcgctaac ttcattgccg tacatgaaat gctggatggc
      421 ttccgcactg cactgatcga tcatctggat accatggcag aacgtgcagt gcagctgggc
      481 ggtgtagctc tggggaccac tcaagttatc aacagcaaaa ctccgctgaa aagttacccg
      541 ctggacatcc acaacgttca ggatcacctg aaagagctgg ctgaccgtta cgcaatcgtc
      601 gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg acgacaccgc agatatcctg
      661 accgccgcgt ctcgcgacct ggataaattc ctgtggttta tcgagtctaa catcgaataa
      721 atccatcgct gatggtgcag aactttagta cacgataaaa gcggcttcct gacaggaggc
      781 cgttttgttt tgtagcccgc ctcaacgcac ttatttagtg catccatctg ctatccccag
      841 ctggttaagt aaattttttg tatccacatc atcacacaat cgttacataa agattgtttt
      901 ttcatcaggt tttacgctaa ataatcactg tgttgagtgc acaattttag cgcaccagtt
      961 tggtgcccca gaatggtgca tcttcagggt attgccctat aaatcgtgca tcacgttttt
     1021 gccgcatctc gaaaaatcaa tgagttgcaa aactggcacg attttttcat atatgtgaat
     1081 gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat gctatgaagt ctgtattaaa
     1141 agtttcactg gctgcactga ccctggcttt tgcggtttct tctcatgccg cggataaaaa
     1201 attagttgtc gcgacggata ccgccttcgt tccgtttgaa tttaaacagg gcgataaata
     1261 cgtgggcttt gacgttgatc tgtgggctgc catcgctaaa gaactgaagc tggattacga
     1321 actgaagccg atggatttca gtgggatcat tccggcactg caaaccaaaa acgtcgatct
     1381 ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa gcgatcgatt tctctgacgg
     1441 ctactacaaa agcggcctgt tagtgatggt gaaagctaac aataacgatg tgaaaagcgt
     1501 gaaagatctc gacgggaaag tggttgctgt gaagagcggc actggctccg ttgattacgc
     1561 gaaagcaaac atcaaaacta aagatctgcg tcagttcccg aacatcgata acgcctatat
     1621 ggaactgggc accaaccgcg cagacgccgt tctgcacgat acgccaaaca ttctgtactt
     1681 catcaaaacc gccggtaacg gtcagttcaa agcggtaggt gactctctgg aagcacagca
     1741 atacggtatt gcgttcccga aaggtagcga cgagctgcgt gacaaagtca acggcgcgtt
     1801 gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac aaaaaatggt tcggtactga
     1861 accgaaataa taacgctgcg cctgtaaagc gcactggcag ttccctctcc ccgccgggga
     1921 gaggattagg gtgaagggcg caaacccgct ccggggccat taattaccct gaatttgatt
     1981 atttacacca cggtaacagg aacaacatat gcagtttgac tggagtgcca tctggcctgc
     2041 cattccgctt ctgattgaag gtgccaaaat gaccctgtgg atttcggtcc tcggtctggc
     2101 tggcggtctg gtgatcggat tgttggcagg ttttgcacgc accttcggtg gttggatagc
     2161 caaccacgtc gcgctggtct ttattgaagt gatccgcggc acacctatcg tcgtccaggt
     2221 gatgtttatc tatttcgccc tgccgatggc gtttaacgat ttacgcatcg acccgtttac
     2281 tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac attgcggaaa tcacgcgtgg
     2341 tgcggtgctg tctattcaca aaggttttcg tgaagcagga ctggcgctcg gtctttcacg
     2401 ttgggaaacc attcgctacg tcattttacc gctggcactg cgtcgtatgc tgccgccgct
     2461 gggtaaccag tggatcatca gcattaaaga cacttcgctg tttattgtta tcggcgtggc
     2521 ggaactgacc cgtcaggggc aagaaattat tgccggtaac ttccgcgccc ttgagatctg
     2581 gagcgccgtg gcggtgttct atctgattat taccctggtg ctgagcttta ttctgcgtcg
     2641 tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa acgtctccaa gcattttggt
     2701 ccaacccagg tgctgcacaa tatcgatttg aacattgctc agggcgaagt cgtggtgatt
     2761 atcgggccgt ccggttccgg taaatcgacc ctgctgcgct gcatcaacaa actggaagaa
     2821 atcacctccg gcgatctgat tgtcgatggc ctgaaggtta acgatccgaa agttgatgag
     2881 cgcctgattc gccaggaagc gggtatggtg ttccagcagt tttacctctt cccgcatctg
     2941 acggcgctgg aaaacgtcat gtttggcccg ctacgcgtgc gtggcgcgaa taaagaagag
     3001 gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc tggcagagcg tgcacatcac
     3061 tacccttccg aactttctgg tggtcaacag cagcgtgtgg cgattgcccg cgcgctggcg
     3121 gtgaagccga aaatgatgct gtttgatgaa ccgacttccg ctcttgaccc ggaactgcgc
     3181 catgaagtgc tgaaggttat gcaggatctg gctgaagaag ggatgacgat ggtgatcgtg
     3241 acccacgaaa tcggttttgc cgagaaagta gcttcgcgcc tgatctttat cgacaaaggc
     3301 cggattgcag aagatggcga tccgcaggtg ttgatcaaga acccgccgag ccagcgcttg
     3361 caggaatttt tgcagcacgt ctcttaactt ctctgcctcc ctttcccccg aaagggaggc
     3421 acaccagatt cctctcattt aaaattgcct ctcccccagc atctatactt atctttttgc
     3481 tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg
     3541 gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca acggactcaa
     3601 cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa agcggcctat ggcgcactgg
     3661 cggatgtgct ggataatgac acctcgcgta aagagttgat cgaccagttg cgcaccgtcg
     3721 ccgcgacgcc ccctgcggaa ccggtaccga agatcgtgcc gccgacgctg gttgaagagc
     3781 aaaccgtgct gcaaaaggtc accgaagtca gccgccatta tggtgaagcc ctttccgccc
     3841 gcttcgggca actttatcgc aatatcaccg gctccccgca taagccgttt aatccacaaa
     3901 ccttcagcaa tgcgctgacc cacttttcaa tgttagcggt attagtgttt ggtttttact
     3961 ggctgattcg tctgtgcgca ctgccgctgt attgcaaaat gggccagtgg gcgcggcaaa
     4021 aaaatcgtga gcgcagtaac tggttgcagc ttccggcgat gatcatcggg gcgtttatta
     4081 tcgacctgct gttactggca ctgacattat ttgtcggcca ggtattaagc gacaacctga
     4141 atgcgggcag tcgcaccatc gctttccaac aaagtttgtt tctcaacgcc tttgccctga
     4201 ttgaattttt caaaaccgta ctacgcctga ttttttgccc aaacgtagct gaactgcgcc
     4261 cgttcacgat tcatgacgag accgcccgtt actggagtcg tcgcctgagc tggttaagca
     4321 gcctgattgg ctatggtctg attgtggctg tgccgattat ctctaatcag gtgaatgtac
     4381 agataggtgc gctggcgaac gtcatcatta tgctgtgcat gaccgtctgg gcgttgtacc
     4441 tgatttttcg taataaaaaa gagatcaccc agcatttgct caactttgcg gagcattcgc
     4501 tggccttttt cagcctgttt atccgcgcct ttgcactggt gtggcactgg ctggcaagcg
     4561 cctattttat cgtgctgttt ttcttttcgt tgttcgatcc gggcaacagc ctgaaattta
     4621 tgatgggtgc aacggtgcgc agcctggcga ttattggtat cgcagcattt gtttccggta
     4681 tgttttcccg ctggctggcg aaaaccatca ccctctcgcc acatactcag cgaaattatc
     4741 cggagctgca aaaacggctg aatggatggc tttcggcggc actgaaaaca gcgcgtattc
     4801 tgacagtctg cgtggcggta atgctgctgt tgagcgcatg gggattgttc gatttctgga
     4861 actggctgca aaacggtgcg gggcagaaaa ccgtagatat cctgatccgt atcgcactca
     4921 ttcttttctt ctcggcggtt ggctggacgg tgctcgccag tttgatagaa aaccggctgg
     4981 cttcggatat tcatggccgc ccgctaccca gcgcccgtac gcgtaccctg ctaacgctgt
     5041 tccgtaacgc gctggcggtg attatcagta ccatcaccat catgattgtg ttgtcggaaa
     5101 tcggcgtcaa tatcgcgcca ttgctggcag gtgccggtgc attgggactg gctatctctt
     5161 ttggttcgca aacgctggtg aaagatatta tcaccggggt atttattcag tttgaaaacg
     5221 gcatgaacac tggagatttg gtgactatcg ggccgttgac cggcactgtg gaacggatgt
     5281 caattcgctc cgtgggcgtg cgccaggata ccggggcgta tcacatcatt ccgtggtctt
     5341 cgataaccac ctttgctaac ttcgtccgcg gtattggttc ggtagtggca aactacgatg
     5401 ttgatcgcca tgaagatgct gataaagcca atcaggcact gaaagatgcg gtagcagaat
     5461 tgatggaaaa cgaagaaatt cgcgggctgg ttattggtga accgaatttt gccgggattg
     5521 tcggcttaag caataccgcg tttacactgc gtgtttcgtt caccacgctg ccactcaaac
     5581 agtggacggt acgctttgcc ctcgacagcc aggtgaaaaa acatttcgac ctggcgggtg
     5641 ttcgcgcgcc ggtgcagact tatcaggtgc tgccagctcc gggcgcggcc ccggctgaac
     5701 cgttgccgcc gggggagcca acgctttaac gctggcgatt gacaaaacgg cggcgctgct
     5761 cgtcgttcat aaaggtccag gctataaagc ggctctgctt ttgcccctgg gccatctctt
     5821 tttttaccac cttcaccgca cctacgtccg tcaaagcacg atacaacggc ggtaagtttt
     5881 caccacgaga taccagcgag gtaaaccaca tcacctgctt cgcgaagcct ttgctctctt
     5941 caatcatctt tttgataaaa gcgacctcac cgccttcaca ccacaactcc tgttgctgac
     6001 caccaaagtt cagtgcatcg tctttgttca gccccaggtt acggcgttta cgttcactac
     6061 ctgcccgtgc cgcagcggcg gaatcgtgga atggcgggtt acacaaggtc gcatcgtatt
     6121 gctcgttttt atggatgatg ccgttaaaaa tcgccccact ctctttttgc cgacgcagac
     6181 gaatggcgcg gttaagcccc ggattagcac tgatgatcgc ctgcgcactg cttaacgcct
     6241 ggctgctggt ttcactaccg gtaaaacgcc agccatattc atgtacgcca atcagcggat
     6301 aaatacagtt cgcaccaacg ccgatatcca gaatgctggc attagctgga attgttccgc
     6361 ttgcttcggc cagtaaatcg gcaaggtgat gaatataatc cgcccggccc ggtactggtg
     6421 ggcagagaaa accgtccggg atatcccagt tcgctacggc gtaaaaatgg gccagcaacg
     6481 ccttattgag cgccttcacc gccagcggat tggcaaagtc tacgctttgc tccccggcgg
     6541 gtgtaagcgt gaggaattgc ctgagttcag gattgacctg acaaagcgtg gcgagatcgt
     6601 agcggctgtg atgacggttg cgcggatgca accccggttt ctgggcggac atagcattct
     6661 cctttaagca gcggcgtaag atacccgcat aacgcgacag ggtaaatatc tggctgcgtt
     6721 tatcatttct aaaaaccttc acatggctat ggtttatggc aattcaccat aaaaaacatc
     6781 atcatgcatg tcattgattt atcttatttt gtaaaaacac atcgttcaaa aaataagcgc
     6841 atctctgtca attttttgcg tcattttcat cgcgcaggcg acctacactt atctggcact
     6901 acccgttgta tttatgagga tgtcattatg aaaaagtgcc tcacactact gattgccacc
     6961 gtcctgagcg gaatctctct cacggcttat gccgcgcaac cgatgagtaa cctggacagt
     7021 ggtcaactac ggcccgccgg taccgtttcg gcaactggcg catcaaacct aagcgatctg
     7081 gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga aaggttatgt cattaattcc
     7141 gcaggcggaa atgaccagat gttcggtact gcaatcatct acaaataacc ccaccaacgt
     7201 aactgcattc gcctctggat gcagttttcg tccaatttgt aagcaaaaaa catcaaatat
     7261 gaacgcaatg taaataaatg tatttctttt tcgcgcaatg ggtgatagaa aatcgcgcca
     7321 agtgataatg cttatcaata ttattatcac ttgcacgtac gctatcacgg gattaacagt
     7381 ggcatcgcat ccgcagagat gctttctcgt gacagtgaaa atttcaccat ataagaaaaa
     7441 gtcacctgca aaatggaaaa caatcgcaat ttccctgcca gacaatttca ttcgctcacg
     7501 ttctttgccg gtctttgtat tggtattacg cctgtggcac aggcactcgc tgccgaaggg
     7561 caagctaacg cggatgacac gctggttgtc gaagcatcaa cgccttcgct ttatgcgcca
     7621 caaaaatctg ccgatccgaa attctcgcgt ccggtagcgg atactacccg cacgatgaca
     7681 gtgatttctg aacaagtgat taaagatcag ggtgcaacca accttaccga cgcgctcaaa
     7741 aacgtccccg gcgtgggtgc gttttttgcg ggtgagaacg gtaactccac cactggcgac
     7801 gccatttata tgcgcggtgc cgatacctct aacagtattt atatcgatgg cattcgcgat
     7861 atcggcagcg tctcgcgcga caccttcaat accgagcagg tcgaagtaat taaagggccg
     7921 tccggcaccg actacgggcg cagcgcaccg acaggctcga tcaatatgat cagcaaacag
     7981 ccgcgcaatg attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc
     8041 ggcacgctgg acgtcaatca ggtcattggt gataccactg cggtgcgcct gaatgtaatg
     8101 ggcgaaaaaa cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc
     8161 ccttctgtcg cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc
     8221 acccagcaca acacgccaga cggcggcatt ccgaccatcg gtttaccggg tttttctgcc
     8281 ccatctgcgg gaacagcggc cctgaatcat tccggaaaag ttgatacgca taacttttac
     8341 ggcacggatt ccgattacga cgattcgacc accgacaccg ctaccatgcg ttttgagcac
     8401 gacatcaacg ataacaccac cattcgcaac actacgcgtt ggtcgcgcgt aaagcaggat
     8461 tacctgatga cggcgattat gggtggagcg tcgaatatta cccagcccac cagcgatgtg
     8521 aatagctgga cgtggtcacg cacggcgaat accaaagatg tgagtaacaa aattctcacc
     8581 aaccagacca acctgacctc gacgttctat accggcgcta tcggtcatga tgtcagtacc
     8641 ggcgtggaat ttacccgcga aacacagact aactatggcg ttaatccggt gacgttaccg
     8701 acggtaaata tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc
     8761 gcaaacgcca atggtcagac ggataccttc gcaatttatg ccttcgatac gctgcaaatc
     8821 acccgtgatt ttgagctgaa cggtgggatc cgtctggata attatcatac tgaatatgac
     8881 agtgccaccg cctgcggcgg cagcgggcgc ggtgccatca cctgccctgc tggcgtggca
     8941 aaaggttctc cggtaaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa
     9001 gccggggcgc tgtatcacct gacggaaaac ggcaatgttt atattaacta tgccgtttcc
     9061 cagcagccgc cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc
     9121 aaccgcaccg attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag
     9181 gttctggata aacgcctgct acttaccgcc gcgctgttcc gtactgatat tgaaaatgaa
     9241 gttgagcaaa atgatgatgg gacttattcg caatatggta agaaacgtgt cgaaggctat
     9301 gagatatccg tggccgggaa tatcaccccc gcgtggcaga tgattggcgg ctatacccag
     9361 caaaaagcaa ctatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg
     9421 tataccccgg aacacgcctt caccttatgg agccagtatc aggcaaccga tgatatttct
     9481 gttggtgcgg gcgcacggta tatcggcagt atgcataaag gttcagacgg cgcggtggga
     9541 acgccagcat ttaccgaagg ttattgggtc gccgacgcca aactggggta tcgggttaat
     9601 cgcaatctcg acttccagct aaacgtctac aacctgtttg ataccgatta cgtcgcctca
     9661 atcaataaga gcggctaccg ttatcacccg ggcgagccaa gaaccttctt gctcacagcc
     9721 aatatgcatt tctgattcag atgtggggcg caggccccac tttttggaga acttgtatga
     9781 tgtaccacat tcccggcgtg ttatcgccac aggacgtcgc ccgttttcgc gaacacctgg
     9841 aacaagccga atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga
     9901 acaatcaaca agtagacacc cgcagcgccc tgtacgccgc cctgcaaaat gaggtgctga
     9961 acgcggttaa ccaacatgct ttgttctttg ccgcggcctt gccgcgtacc ctttccacgc
    10021 cgctgtttaa tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac
    10081 gcagccatcc acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg
    10141 atccagaaag ctacgacggc ggcgaactgg tcgttaatga caccttcggg caacatcggg
    10201 taaaactccc ggcaggcgat ctggtgttgt atccctccag cagcctgcat tgcgtgacac
    10261 ccgtaacccg cggcgtgcga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg
    10321 ataaaaagcg cgccatgctg tttgaactgg acaacaatat tcagtcgctg aaaagccact
    10381 acggcgaaag tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat
    10441 ggtcggagat ctgacgcttg aactgcccga gttcgtccct atattagcta cacttaactg
    10501 tacaagcatt gatatgggga ggacgatatg gcatccggtt gggctaatga tgacgccgtc
    10561 aacgaacaga tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg
    10621 cgtggcgaaa gcctgtatga atgtgaagag tgcggtgccc ctatcccgca ggcccgtcgg
    10681 gaagccattc ctggcgtgcg cttatgcatt cattgtcagc aggagaaaga tttacaaaaa
    10741 ccagcttata caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa
    10801 ctggcgggat gtatagcgaa tataatttgt attttgcgtg gcattccgtg cctttaacgc
    10861 taccgtgact gccacgcggt ataaaacaag tccataagta caacaaataa atggtttatc
    10921 agtatgttag atatcaatca atttatttga acaaggtggt caattctctt cgattttatc
    10981 tctcgtaaaa aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc
    11041 acctgcgaga gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca
    11101 actctgtcat ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg
    11161 aataaaatcg gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa
    11221 ctggctgaga aagccgcggc ggccggtgcc agtggataca gcatcacttc tgccaccaac
    11281 aacaataaat taagcggtac tgcggtaatt tataagtaat tcgtaagctg gatcaggcgt
    11341 ttacgctacg ttcagcacaa gtatttccct gtctgtttgc cgacagacgc atatgctcta
    11401 accctcatga tcctatgtta cccttgtttg cccgtccgcc actggacggg ctttttttag
    11461 ctggctagct gctgacagaa agcctgcaac gtctcttccg gcataccaat ctgccgcgcc
    11521 gcatcacaaa tggcctgcca gcttcccgca tccagtggaa tcccctgctc ctgccgttcg
    11581 cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg gcttatcgtc atcatgcggc
    11641 gaggctttca cccactcggc aaaggcttcg gtctgcgcac tacaatccgg cgcgccgaac
    11701 agttccgggt tgataatgat agtggtcatg cagttaagaa tggcatcggg actggtttgt
    11761 aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc cgccaagaat ttcacacatc
    11821 gctgcaaggg cgtagccttt atgttcggca aaggtcaaca gcgaacccaa cggcgactcc
    11881 tgcattaccg ccggatttgt cgtcggcacg ccgttaacgt caatcaggca acctggcggc
    11941 acggggacgc ctttatgcca ggcgacgcgg gttttgccaa atgcaatggc gctggtggcg
    12001 taatcgagca acagcgggaa attatcttta cgagggaaaa ccacacagaa cggattggtg
    12061 ccaaagcggc tgtcgcgacc gtggaacggc gcgaccattg gaataccgac cacgctaaca
    12121 aagtggatag agacaaaccc cgccgctgca cactgctccg cccagtaacc gatacggccg
    12181 atatgatgcg agttatgtag cgccacggcg gcaatgccgt gctgatgcgc tttctcaatc
    12241 cccagcgcca tcgcttcgtg tgccgcgacc tgaccaaatg cgcgatcgcc atcgagcgtg
    12301 accgccgccc ccgcttcttt aacgactttg gcatgatggt taatttgcag atgcccctga
    12361 ctccaggagc gcacatagct tgggatcatg ccaataccat gtgaatcatg ccctgccagg
    12421 tttgccgcga tcaaatgatc ggcgactaat ttcgcttcct gttcctcact acccatctga
    12481 cgaaatacag cctgaataaa actgtgcagc gtctgagcat caaagcgatg gccactttcc
    12541 atggttaact cctgtttatg ttatgtgttt gttgtttttt atgctgcacg ccgggcgcgg
    12601 gaaggtcaat aaagatgcag gaatttcata ttgcggtggg gtaaaagccg gatgacatgg
    12661 ctcatccggc tgagataaaa attaaaatgc ctgattaacg cgcgccaggc cgtcgctgat
    12721 agttgccgct tcacccgtag ccatcaggca gcaagccatc tggattttca gcgattcggg
    12781 aatcggttcg ctgccagcca ggcaacgctc aatccactgc gccgtggttt ccggatcttt
    12841 tgcttgtggc agtaactcgc taccagcagt gtcctgtttt tcatacagta cccgcatccc
    12901 ttcacggtca atgagattga tctgcgggca gcgctgcgga ttagcataca cttcaccttc
    12961 agtaccatgc ataagtagcg cccgcccgcc gatatcgcta aagaacttcg cgacgcgtcc
    13021 aatgtattcc ggatgcgaaa cgctggaaag acgcagtgcc tcaccttcgg caaatggcgt
    13081 cgccagtttc gccagggtat gtgcactgtt acgcacgccc atccgccagc gcatcgccag
    13141 ttgtttttcc agcggcgggc aaaacgcgcc gacaggcata aagacaggtt gatgttcgtc
    13201 gagcttcgcc tgcgcctgcc cgccgtgcaa cgttggcgta atacccatca gttcgaaaat
    13261 ggtttcggtc agcacgcggg ttggatcttc gctaaccccg tgaaccacca caggaaaacc
    13321 gagtttgtgc agcagaatcg ccagcaacgg cgtcaggttg gcctgtttac gcgcgccgtt
    13381 gtaactgggg atgacaatcg gcatcggctt gcctgcaggc ggcgtcagct tgatggtgtg
    13441 attttgcatg gcttcgtaaa agccgagcat ctctgcttcc ccttcccctt tgatacgtag
    13501 cgcaatcaat acgccgccca actcaaggtc agggacttca ccattgagca tatgggcata
    13561 cagaccgcgc gcagtatccc ggtctaaatc gcgcgcgtgg tttttcccgc gcccgatctc
    13621 tttaatgatt ttgcgatagt ccatttacga ctccttacct gactcacatc attaacgccg
    13681 ccgacggcgt ggggatttcg ttttttcttt ctttttaact ataccttcag ggacttccgg
    13741 ttgctctatc ggaaataccg gtaatgcatc cagtagtcgc ttgccataat tttttgtcag
    13801 caagcgtttg tcgtagataa ccacttcccc ccagcaaccg tggcttcgaa tcagtcgccc
    13861 aacctgctga atcaggttaa acgaagcgct cggcaggctt tgcacctcaa acggatagcg
    13921 gttaaggctt ttcagccatt caccttcggt gatcaccacc ggactgtcga tgggcggaaa
    13981 agcgattttg tggatatgca cctggctgag cagagcgcct ttcagatcaa gaccttcggc
    14041 aaaggactgt aagcccacca gcacgctacg ctcaccgttg gcaacgcgtt tgcggtgcag
    14101 ttcaactaag cgataacgtg gctgatcgcc ctgcaccagc aacatcaggc gtaaatccgt
    14161 cacatagtcg agaaagcgct gcatcgcacg cccgctggca aacagcacca acataccgag
    14221 atgttttttg ctctccacct gctcacggaa aaaggccgcc atttcagcaa tgtgctgttc
    14281 ttcgttgtcg atggaaggct caacgcgcat ccggggaata acaattttgc cctgttcgca
    14341 gtggttaaag ggcgaatcca gcgccacaaa gcggtcaccc gctttctctt tcagcccact
    14401 catctcctgc aaacgcgaaa aactgttcag cgaacgcaag gttgcggagg taacaataat
    14461 gtgcggaata ctgcgccaca gcagcctttc cagttggtcg ctaacacgaa tacccacaca
    14521 gtgaaaccac agatgtagct gcccttcgcg ctcttcccgc gtcgcccatt tggtcaccgg
    14581 cgcaccggaa gattgcgcca gagacgccag ccgccagagt ttgctttgcg cctcgaacat
    14641 ccccaacgcg cggttcatct gcaaaatcaa ccgatgcagg cgtacaatgt catggctgcc
    14701 ggttttctca cttaaatcgt taagaaataa ctccgccaga ccacgcagca tctcggtgag
    14761 ttttgccagc cgctggcaga tctccagcac ttcatcaggc agttcgccca tcgcaaaacg
    14821 gtgctctgcc tcctgcccgg caggcatgta gagattgaga atgttgttta acgaggcgat
    14881 aagctcatac agctcttcac aatgcgcatt caaacgttca gggatcgcca gcggtgggat
    14941 ggtcttcggg cgaaactgct ccatgcaggt ggcgaccagt ttggtgaaca ggtccagttg
    15001 gagccggtac cagggcgcag tgatttcggc gctcatctcc agcgcatccc gcgccacatc
    15061 cggcaggtga tgaccttcgt ccagcaccag cagtaaattt ttcgggtcag gcaataccgc
    15121 ttcgctttcc attgccgcca tcaccagcgc atggtttgcc accaccactt ccgcttcctg
    15181 aatttcccga cgagcgacaa aaaacgggca ttcgcgatag tagtaacagt tgcggttgag
    15241 gcagctggct ttgtcggtac tcaaacgacg ccagagatca tcatctatag cgatatcagt
    15301 atgatcgcgc aggccatccc atttataagt gtcgagatcg cccttcagct tcgcacaacg
    15361 tttttgctct tcctgattgt tcggcgtcag ttcgtcgtca agaaacgcca gcagatcctg
    15421 ttgcgtgggc tcagtgctgg cgagcgctgt cagattacgc gggcaaacgt agcgcccacg
    15481 cccaaaagcg gcagtgaatt taagatcggg aatgatcttt ttcagcagcg gtaaatcttt
    15541 gctgtagatc tgatcctgca atgccacgtt ggcggtactt accaccagcg ttttttgctc
    15601 ttcgcgggca atggcgatgc cggggatcaa ataggagagc gttttcccaa cgccggtagg
    15661 agcttcaatc gccagatgcc gcccttcttc tccggccagc gttttggcga cgtccgcaat
    15721 catctgccgc tgcggcgcac ggggaataaa gtcggggatc tgttcctgaa gcgccttata
    15781 ccaggcggca atttgcgctt taagcgcggc ggttaatgcc atgagaaaac ctgaaatact
    15841 gtataaacag ccaatattgt ggcatttttc ggttttcagg ggtaatggtt tttctgtttc
    15901 tggaaagcgg ctcgcaataa ccagcaggat gatgcgtaaa gcgcttcgcg ctaaattcga
    15961 acaacatgca gaactgcgta cactcttgct ggcaaccgcg tccgcaaaac tggttgagca
    16021 tacgcaaaat gatgcttact ggggagatgg cggtaatggt cagggcaaga atcgcctggg
    16081 gtacctttta atggcgttgc gcggacaatt ggctgcggag aagtaacgca tcgggtggct
    16141 tgtgggcgtt aaaacagaga aatctttttg ccgcgctcgc aaatggctga caagtgcttg
    16201 ttgtgaaatg ccggatgcgg cgcgagcgcc ttatccggcc tacaaaaaca tgcacattca
    16261 atatattgca gagttcacgt aggcctgata agcgtagcgt atcaggcagt tttgtgtttg
    16321 tcatcagtct catgccgggc atagcccggc ataaaagatt actgcgcagc ggcaggttta
    16381 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag acgacgagac
    16441 ggtttttctg cgggcttcgc gttaccagac ttcgcaccac tttccgcacg gcgtggttgc
    16501 tgttgaccgc gaccaccgcc ctgtccacga ccgccgccac gttgctggcg accgttttgg
    16561 atcggttcgg ctttgattga cgggtccggc tcatagccca gaatcgcaat gcgcgggatc
    16621 tcttttttca gcagtttttc gatatcacgc agcagtttgt gttcatcaac acacaccagc
    16681 gacaatgctt cgccagttgc cgccgcacgg ccagtacgcc cgatacggtg aacataatct
    16741 tccggtacgt ttggtaactc atagttgacg acgtgcggca actcttcgat atccagacca
    16801 cgcgcagcga tgtcagttgc caccagtaca cgaatatcgc ccgatttaaa atcggccagc
    16861 gcacgagtac gcgcaccttg cgatttatta ccgtggatcg ccgcactacg gatgccatct
    16921 ttattaagct gttcagccag atggttagcg ccatgtttgg tacgggtaaa caccagcacc
    16981 tgctgccagt tccctttccc aatcatatgc gacagcaatt cgcgtttgcg tttcttatcg
    17041 acaaagtgaa cgtgctgggt cacctgatca gacgcagtat tgcggcgtgc cacttcgatt
    17101 tccagcgggt tgtgcaacag tttttccgcc agggctttaa tatcgtcaga gaaggtcgcg
    17161 gagaacaaca ggttctggcg cttcgctggc agttttgtta acacgcgacg gatatcgtgg
    17221 ataaagccca tgtcgagcat gcggtcagct tcatcaagga cgaggatttc aacctgatcc
    17281 agcttcactg cattttgatg ttccaggtcc agcagacgtc ccggcgttgc caccagcaca
    17341 tcaacgccgc cgcgcagctt catcatctgc gggttaatgc tgacaccgcc aaacaccacc
    17401 agcgaacgaa tgttcaggta tttgctgtaa tcacggacgt tttcgccaat ctgcgccgcc
    17461 agttcacggg tcggggtaag aatgagcgca cgcaccggac gacgcccttt ggcgtgcggc
    17521 tggcgagtga tcaggtgttg caacagcggc agcgtaaagc ccgctgtttt acctgtaccg
    17581 gtctgggcgc tggccatcag gtcgcggcct tccagcaccg cagggatcgc ctgctgctga
    17641 ataggggtgg gttcacggta accctgctcg gcaacggcgc gcaggatatc agggcttaaa
    17701 cccaaagaat cgaaagacat aactactccg aaccgccccg accgttaccg gtgtagtttt
    17761 cagggagata ctgaaaagga aaatgacaaa aaccacaatg tcatgaggga gcggagtgta
    17821 gcagtttttg tgacgcagcg cataaattat ccctgtgact ggcgtagcta aaattcttag
    17881 ccatactgga caactcccct atggagtcat aatcttaatc aatcatttga ttaagaatgg
    17941 actgtgcgat gaataatcct gccatgacaa tcaagggtga acaggcgaaa aaacagctga
    18001 ttgctgcggc actggcacag tttggtgaat atggaatgaa cgccaccacc cgcgagatag
    18061 ccgcccaggc cgggcagaat atcgctgcca tcacctacta cttcggttcg aaagaagatt
    18121 tgtacctcgc ctgcgcccag tggattgctg attttattgg cgagcagttc cgtccgcacg
    18181 ccgaagaagc cgaacgtttg ttcgcgcaac cacagccgga tcgggctgcc atccgtgaac
    18241 tgatccttcg cgcctgcaga aacatgatta agctgctcac ccaggatgac accgtcaacc
    18301 tcagcaaatt tatctcccgt gagcagctct ctcccacggc agcctaccac ctggtgcacg
    18361 aacaggtgat tagtccgcta cacagtcacc ttacacgcct gattgccgcc tggaccggct
    18421 gcgacgctag cgatacccgc atgatccttc atacccatgc gttgattggt gagattctgg
    18481 cgtttcgtct tggcaaagaa actattctgt tacgtaccgg ctggaccgcg ttcgatgaag
    18541 aaaagaccga actgatcaac cagacggtga cctgtcatat tgacctgatt ttgcaaggat
    18601 tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg atcggactgg cggtagtagt
    18661 acttgccgcc gtggttgccg gaggctactg gtggtatcaa agccgccagg ataacggcct
    18721 gacgctgtat ggcaacgtgg atattcgtac ggtgaatctt agtttccgtg ttggggggcg
    18781 cgttgagtcg ctggcggtgg acgaaggtga tgcgatcaaa gcgggccagg tgctgggcga
    18841 actggatcac aagccgtatg agattgccct gatgcaggcg aaagcgggtg tttcggtggc
    18901 tcaggcgcag tatgacctga tgcttgccgg gtatcgagat gaagaaatcg ctcaggccgc
    18961 cgcagcggtg aaacaggcgc aagctgccta tgactatgcg cagaacttct ataaccgcca
    19021 gcaaggactg tggaaaagcc gcaccatttc ggcaaatgac ctagaaaatg cccgctcctc
    19081 gcgcgaccag gcgcaggcca cgctgaaatc agcgcaggat aaattgcgtc agtaccgttc
    19141 cggtaaccgc gaacaggaca tcgcccaggc gaaagccagc ctcgaacaag cacaggcgca
    19201 actggcgcag gcggagctga atttgcagga ctcaacgttg atagcaccgt ctgatggcac
    19261 gctgttaacg cgcgctgtag agccaggcac ggtgcttaat gaaggtggca cggtatttac
    19321 cgtctcgctg acgcgtccgg tgtgggtacg cgcctatgtt gatgaacgta atcttgacca
    19381 ggcccagccg gggcgcaaag tgttgcttta taccgatggt cgcccggaca agccgtatca
    19441 cgggcagatt ggtttcgttt cgccgactgc tgaatttacc ccgaaaaccg tcgaaacacc
    19501 ggatctgcgt accgacctcg tctatcgcct gcgtattgtg gtgaccgacg ccgatgatgc
    19561 gttacgccag ggaatgccgg taacggtgca attcggtgac gaggcaggac atgaatgatg
    19621 ccgttatcac gctgaacggc ctggaaaaac gctttccggg catggacaag cccgccgtcg
    19681 caccgctcga ctgcaccatt cacgccggtt atgtgacggg gctggttggg ccggacggtg
    19741 cgggcaaaac cacgctgatg cggatgctgg cgggattact gaaacccgac agcggcagtg
    19801 ccacggtgat tggctttgat ccgatcaaaa acgacggcgc gctgcacgcc gtgctcggct
    19861 atatgccgca gaaattcggc ctgtatgaag atctgacggt gatggagaac ctcaatctgt
    19921 acgcggattt gcgcagcgtc accggcgagg cacggaagca aacttttgct cgcctgctgg
    19981 agtttacgtc tcttggcccg tttaccggac gcctggcggg caagctctcc ggtgggatga
    20041 aacaaaaact tggcctggcc tgtaccctgg tgggcgaacc gaaagtgttg ctgctcgatg
    20101 aacccggcgt cggcgttgac cctatctcac ggcgcgaact gtggcagatg gtgcatgagc
    20161 tggcgggcga agggatgtta atcctctgga gtacctcgta tctcgacgaa gccgagcagt
    20221 gccgtgacgt gttactgatg aacgaaggcg agctgctgta tcagggagaa ccgacggccc
    20281 tgactcaaac catggccgga cgcagctttc tgatgaccag cccgcacgag ggcaaccgca
    20341 aactgttgca acgggcattg aaactgccgc aggtcagcga cggcatgatt caggggaaat
    20401 cggtacgtct gatcctcaaa aaagagacca caccagacga tattcgccat gccgacggga
    20461 tgccggaaat caacattaac gaaactacgc cacgttttga agatgcgttt attgatttgc
    20521 tgggcggtgc cggaacctcg gaatcgccgc tgggcgcaat attgcatacg gtggaaggta
    20581 cacctggcga gacggtgatc gaagcgaaag agctgaccaa aaagtttggc gattttgccg
    20641 ccaccgatca cgtcaacttt gccgtcaaac gcggtgaaat ctttggtttg ctcgggccaa
    20701 acggcgcagg gaaatcgacc acctttaaga tgatgtgcgg tttgctggta ccgacctctg
    20761 gtcaggcgct ggtgctgggc atggatctga aagagagttc cggtaaagcg cgccagcatc
    20821 tcggctatat ggcgcaaaaa ttttcgctct acggcaacct gacggtcgaa cagaatttac
    20881 gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca gaacgaaaaa atctcccgta
    20941 tgagcgaggc gttcggcctg aaaagtatcg cctcccacgc gaccgatgaa ctgccattag
    21001 gttttaaaca gcggctggcg ctggcctgtt cgctgatgca tgaaccggac attctgtttc
    21061 tcgacgaacc gacgtccggc gttgatcccc tcacccgccg tgaattttgg ctacatatca
    21121 acagtatggt agagaaaggc gtcacggtga tggtcaccac ccactttatg gatgaagcgg
    21181 aatattgcga ccgcatcggc ctggtgtacc gcgggaaatt aatcgccagc ggcacaccgg
    21241 acgatttgaa agcgcagtcg gccaacgatg agcaacccga tcccacgatg gagcaagcct
    21301 ttattcagtt gatccacgac tgggataagg agcatagcaa tgagtaatcc gatcctgtcc
    21361 tggcgtcgcg tgcgggcgct gtgcgtcaaa gagacgcggc agatcgttcg cgatccgagt
    21421 agctggctga ttgcagtagt gatcccactg ctactgctgt ttatttttgg atacggcatt
    21481 aacctcgact ccagcaagct gcgcgtcggg attttactgg aacaacgtag cgaagcggcg
    21541 ctggatttca cccacaccat gaccggttcg ccctacatcg acgccaccat cagcgataac
    21601 cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc gcggtctggt ggttattccg
    21661 gtggatttcg ccgaacagat ggagcgcgcc aacgccatcg caccgattca ggtgattacc
    21721 gacggcagtg agccgaatac cgctaacttt gtgcaggggt atgtcgaagg gatctggcag
    21781 atctggcaaa tgcagcgagc ggaggataac gggcagactt ttgaaccgct tattgatgta
    21841 caaacccgct actggtttaa cccggcggcg ataagccagc actttattat ccccggtgcg
    21901 gtgactatta tcatgacggt catcggtgcg attctcacct cgctggtggt ggcgcgagaa
    21961 tgggaacgcg gcaccatgga ggctttgctc tctacggaga ttacccgcac ggagctgctg
    22021 ctgtgtaagc tgatccctta ttactttctc gggatgctgg cgatgttgct gtgtatgctg
    22081 gtgtcggtgt ttattctcgg cgtgccgtat cgcgggtcgc tgctgattct gttttttatc
    22141 tccagcctgt ttttactcag taccctgggg atggggctgc tgatttccac gattacccgc
    22201 aaccagttca acgccgctca ggtggccctg aacgcggctt ttctgccgtc gattatgctt
    22261 tccggcttta tatttcagat agacagtatg cccgcggtga tccgcgcggt gacgtacatt
    22321 attcccgcac gttatttcgt cagcactctg caaagcctgt tcctcgccgg gaatattcca
    22381 gtggtgctga tagtgaacgt gctgtttttg atcgcttcgg cggtgatgtt tatcggcctg
    22441 acgtggctga aaaccaaacg tcggctggat tagggagaag agcatgtttc atcgcttatg
    22501 gacgttaatc cgcaaagagt tgcagtcgct gctgcgcgaa ccgcaaaccc gcgcaattct
    22561 gattttaccc gtgctgattc aggtgatcct gttcccgttc gccgccacgc tggaagtgac
    22621 caacgccacc atcgccatct acgatgaaga taacggcgag cactcagtag aacttactca
    22681 acgttttgcc cgcgccagcg cctttaccca tgtgctgctg ctgaaaagcc cgcaggagat
    22741 ccgcccgacc atcgacacac aaaaggcgtt actactggtg cgtttcccgg ctgacttctc
    22801 gcgcaaactg gataccttcc agaccgcgcc gttgcagttg atcctcgacg ggcgtaactc
    22861 caacagtgcg caaattgccg ccaactacct gcaacagatc gtcaaaaatt atcagcagga
    22921 gctgctggaa ggaaaaccga aacccaacaa cagcgagctg gtggtacgca actggtataa
    22981 cccgaatctc gactacaaat ggtttgtggt gccgtcgctg atcgccatga tcaccactat
    23041 cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa cgtgaacaag gtacgctcga
    23101 tcagttgctg gtttcgcccc tcaccacctg gcagatattc atcggcaaag ccgtaccggc
    23161 attaattgtc gccaccttcc aggccaccat tgtgctggcg attggtatct gggcgtatca
    23221 aatccccttc gccggatcgc tggcgctgtt ctactttacg atggtgattt acggtttatc
    23281 gctggtggga ttcggtctgt tgatttcatc actctgttca acacaacagc aggcgtttat
    23341 cggcgtgttt gtctttatga tgcccgccat tctcctttcc ggttacgttt cgccggtgga
    23401 aaacatgcca gtgtggctgc aaaatctgac gtggattaac cctattcgcc actttacgga
    23461 cattaccaag cagatttatt tgaaggatgc aagtctggat attgtgtgga atagtttgtg
    23521 gccgctactg gtgataacgg ccacgacagg gtcagcggcg tacgcgatgt ttagacgtaa
    23581 ggtgatgtaa cttcttatct ttcgccagca aagacaccac cgccgggccg gcaaggattg
    23641 ccagccctgc aattgccagc aaaaagttgt tttccagtac gcgggacaac agccacagca
    23701 caatcagcgc tgcaccaaaa taccaggtcg tggtcagctc ttccagcacg tctgccacct
    23761 cacgccagcg ttgctctggg tggcgctgaa acaccagcat catcactacc gtgacacaat
    23821 acagtgcgca taatcctacg ccgacgtgaa ccagagtttt actcatccaa cccaccacca
    23881 gcagcgccac taccagtaaa tgcaacataa tctgccagca acttaggcca gttgcgacac
    23941 gaacacgttg ttgccacttc atggcttctc tcctgaagaa tttatctcta cttattcaga
    24001 gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt ttgtgacgcc gactacacta
    24061 tttcaatgag ataagaggca aagacaggaa aattatgccc gatcaaacac aacaattttc
    24121 gttcaaggtg ctcaccatca atattcacaa aggctttacc gcgtttaacc gacgcttcat
    24181 tttaccggaa cttcgcgacg ccgtgcgtac cgtcagcgcc gatattgttt gcctgcagga
    24241 agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa aactggcccg atacctcgca
    24301 ctacgagttt ctcgccgaca ccatgtggag cgatttcgcc tacggtcgca atgccgtata
    24361 cccggaagga catcacggta acgccgttct ttcacgttat cccattgaac attatgagaa
    24421 tcgtgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg ctctactgcc gcattgtgcc
    24481 gccgatgacc gggaaagcga ttcatgtgat gtgcgtacat ctgggcctac gtgaggcgca
    24541 ccgtcaggcg cagcttgcga tgctcgccga atgggtgaat gagctaccgg acggcgaatc
    24601 ggtattggta gcgggtgatt tcaacgactg gcggcaaaaa gctaatcatc cgttaaaagt
    24661 gctggccggg ctggatgaga tttttacccg cgcccacgga cgtccggcgc gcacgtttcc
    24721 ggtgcaattt cctctactac gactggacag gatctacgtc aaaaatgcca gcgccagcgc
    24781 gccaaccgcg ttgccgctgc ggacatggcg acacctttct gatcatgccc ctttaagtgc
    24841 ggagattcat ttatgaaatg tagctggcgc gaaggcaata tgatccagtt gctggaaaac
    24901 ggcgagcaat attatcccgc ggtgtttaag gcgattggcg aggcgcaaga acgcatcatt
    24961 cttgaaacgt ttatctggtt tgaggatgac gtcggcaaac agctgcatgc ggcgctattg
    25021 gcagcagcgc aacgcggggt aaaagcggaa gtcttgctgg atggctacgg ttcgccggat
    25081 ctcagcgatg aatttgtcaa tgaactgacg gcagctggcg tggtgttccg ctactacgat
    25141 ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc gccggatgca tcgcaaaatt
    25201 gtggtgatcg acgcgcgtat cgcctttatt ggcgggctga attactccgc cgagcatatg
    25261 tccagctacg gcccggaggc caaacaggat tacgcggtac gcctcgaagg gccgattgtc
    25321 gaagatattc tccagtttga gctggaaaac ctgccaggac aaagcacggc gcgacgctgg
    25381 tggcgacgcc atcataaagc ggaagagaac cgccagccgg gagaagcgca ggtattgctg
    25441 gtctggcgcg ataacgaaga acatcgcgat gatatcgaac gtcactatct gaaaatgctc
    25501 actcaggcgc ggcgggaagt gattatcgcc aacgcctact tcttccccgg ctatcgtttt
    25561 ttacacgcct tgcgtaaagc ggcacgacgc ggggtgcgga tcaaactgat cattcagggc
    25621 gaaccggata tgccgattgt cagagtcggc gcgcgtttgc tgtataacta tctggttaaa
    25681 ggtggcgttc aggtgtttga gtaccgccgc cgcccgctcc acggcaaagt ggcattgatg
    25741 gacgatcact gggcgacagt agggtccagt aatctcgatc cgctcagttt gtcactgaat
    25801 ctcgaagcaa atgtcatcat ccacgatcgt aattttaacc agacgctgcg cgataatctg
    25861 aacggcatta tcgccgcaga ttgtcagcag gtcgatgaga ccatgctgcc gaaacgcacc
    25921 tggtggaacc tgactaaaag cgtgctggcg ttccactttc tacgccactt cccggcgctg
    25981 gtgggctggc ttccggcgca cacgccacgt ctggcgcagg ttgatccccc cgcacaaccg
    26041 acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg gggtaaaacc ctgatgagta
    26101 aatcacaccc gcgctggcgc ttagcaaaga agatcctcac ctggctgttt tttatcgcgg
    26161 tgattgtgtt gttggtggtc tacgccaaaa aagtggactg ggaagaggtc tggaaggtca
    26221 tccgagacta caatcgcgtt gcactgctca gtgcggtcgg gctggtggtc gtcagctatc
    26281 tgatttacgg ttgctacgac ctgctcgccc gtttctactg cggtcacaaa ctggcgaagc
    26341 gccaggtgat gctggtgtcg tttatctgct acgccttcaa cctgacactc agtacctggg
    26401 tcggcggcat tggtatgcgc tatcgtttgt actcccgact ggggttaccg ggcagcacta
    26461 ttacgcggat tttttcgctc agtattacca ccaactggct aggctatatt ttgctagcgg
    26521 gggttatctt taccgcaggc gtggtggagt tgccggatca ctggtatgtc gatcaaacta
    26581 cgctgcgtat tctcggcatt ggcttactga tgattatcgc ggtttatttg tggttttgcg
    26641 ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa gctggtgctg ccttcgtgga
    26701 aattcgccct cgcccagatg ctgatttcca gcgttaactg gatggtaatg ggagcgatta
    26761 tctggctgtt acttggtcaa agcgtaagct atttctttgt actgggcgtg ttgctggtta
    26821 gtagtattgc tggcgtcatc gtgcatattc cggcgggaat tggtgtgctg gaagcggtgt
    26881 ttatcgcgct actggctggg gagcatacct ccaaaggcac aattatcgcc gccctacttg
    26941 cttaccgtgt gctgtattac tttatcccac tgctgctggc gctgatctgt tacctgttgc
    27001 tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc ggcgatgtga agtatagtta
    27061 agttctttag tatgtgcatt tacggctaat gaaaaaaccg cgtatgcctt tgccagacaa
    27121 gcgttatagc tatagcagca agaatcccaa agaaaccgat aaaatagtaa tagagtgtta
    27181 atgcacctat aaaagcacaa cgaaccggac tccataattc ttcactatgg ctttttcctg
    27241 tcagttcaag gagcttattt cgtccatgcg agataatgcc tgaccataag acaatcatta
    27301 agcaacttat tacccaaatg ggtcgttcac tcattaatgt ggcattcacg atgattacca
    27361 gggccaaccc tgtcaacgtc atcataatga taaaacgatg agaaccagga tcaaatttaa
    27421 acaatagacc tgccagcata ctgattaaaa acatcgatcc cgcagtgctg aacacgacga
    27481 taatggcaga tacaggcgct aaactcgcaa cgacaatcgc caccaaagag attaaaaaca
    27541 aatatattac gcccgcgatg gcggtattta tcttgtt
//
LOCUS       DAJOTB010000054        27553 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJOTB010000054 DAJOTB010000000
VERSION     DAJOTB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.054"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1036
                     /locus_tag="OHN28_002240"
     CDS             <1..1036
                     /locus_tag="OHN28_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCQ2297636.1"
                     /translation="LQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGFSALTL
                     AILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGISGIII
                     DVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEASKVTV
                     AVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAISPDAE
                     NAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAIFNSFH
                     LLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLIVGGGA
                     INYVIQSVIA"
     gene            1110..1967
                     /gene="nfo"
                     /locus_tag="OHN28_002241"
     CDS             1110..1967
                     /gene="nfo"
                     /locus_tag="OHN28_002241"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCQ2297637.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1971..3059
                     /locus_tag="OHN28_002242"
     CDS             1971..3059
                     /locus_tag="OHN28_002242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2297638.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3327..4268)
                     /gene="rihB"
                     /locus_tag="OHN28_002243"
     CDS             complement(3327..4268)
                     /gene="rihB"
                     /locus_tag="OHN28_002243"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCQ2297639.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4398..5096
                     /gene="yeiL"
                     /locus_tag="OHN28_002244"
     CDS             4398..5096
                     /gene="yeiL"
                     /locus_tag="OHN28_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCQ2297640.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(5163..6413)
                     /locus_tag="OHN28_002245"
     CDS             complement(5163..6413)
                     /locus_tag="OHN28_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCQ2297641.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6507..7445)
                     /gene="psuG"
                     /locus_tag="OHN28_002246"
     CDS             complement(6507..7445)
                     /gene="psuG"
                     /locus_tag="OHN28_002246"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCQ2297642.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(7433..8374)
                     /locus_tag="OHN28_002247"
     CDS             complement(7433..8374)
                     /locus_tag="OHN28_002247"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCQ2297643.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(8830..10521)
                     /gene="fruA"
                     /locus_tag="OHN28_002248"
     CDS             complement(8830..10521)
                     /gene="fruA"
                     /locus_tag="OHN28_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCQ2297644.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(10538..11476)
                     /gene="fruK"
                     /locus_tag="OHN28_002249"
     CDS             complement(10538..11476)
                     /gene="fruK"
                     /locus_tag="OHN28_002249"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCQ2297645.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(11476..12606)
                     /gene="fruB"
                     /locus_tag="OHN28_002250"
     CDS             complement(11476..12606)
                     /gene="fruB"
                     /locus_tag="OHN28_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCQ2297646.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            12974..14155
                     /gene="setB"
                     /locus_tag="OHN28_002251"
     CDS             12974..14155
                     /gene="setB"
                     /locus_tag="OHN28_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCQ2297647.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(14152..14406)
                     /locus_tag="OHN28_002252"
     CDS             complement(14152..14406)
                     /locus_tag="OHN28_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2297648.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            14561..15133
                     /gene="yeiP"
                     /locus_tag="OHN28_002253"
     CDS             14561..15133
                     /gene="yeiP"
                     /locus_tag="OHN28_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCQ2297649.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            15356..16822
                     /locus_tag="OHN28_002254"
     CDS             15356..16822
                     /locus_tag="OHN28_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2297650.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            16940..17926
                     /gene="yeiR"
                     /locus_tag="OHN28_002255"
     CDS             16940..17926
                     /gene="yeiR"
                     /locus_tag="OHN28_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCQ2297651.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            17965..18678
                     /gene="lpxT"
                     /locus_tag="OHN28_002256"
     CDS             17965..18678
                     /gene="lpxT"
                     /locus_tag="OHN28_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCQ2297652.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            19090..19656
                     /gene="mepS"
                     /locus_tag="OHN28_002257"
     CDS             19090..19656
                     /gene="mepS"
                     /locus_tag="OHN28_002257"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCQ2297653.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            19837..21393
                     /locus_tag="OHN28_002258"
     CDS             19837..21393
                     /locus_tag="OHN28_002258"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2297654.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            21475..23289
                     /locus_tag="OHN28_002259"
     CDS             21475..23289
                     /locus_tag="OHN28_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2297655.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            23290..24384
                     /locus_tag="OHN28_002260"
     CDS             23290..24384
                     /locus_tag="OHN28_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCQ2297656.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            24384..25409
                     /locus_tag="OHN28_002261"
     CDS             24384..25409
                     /locus_tag="OHN28_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297657.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            25411..27000
                     /gene="yejF"
                     /locus_tag="OHN28_002262"
     CDS             25411..27000
                     /gene="yejF"
                     /locus_tag="OHN28_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCQ2297658.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(27004..27348)
                     /locus_tag="OHN28_002263"
     CDS             complement(27004..27348)
                     /locus_tag="OHN28_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297659.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         6714 a         7076 c         6710 g         7053 t
ORIGIN      
        1 cttacagaaa caacatcgta cactgtggca ttttattccg gggttagccc tgagtgcagt
       61 tatcaccggg gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg ccgggtttag
      121 tgccctcacc ctcgcaatct tgttggggat ggttttaggc aacaccattt acccgcacat
      181 ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac gtctgggtat
      241 cattctttat ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta tcagtgggat
      301 catcattgac gtgttgacgc tgtccagtac cttcctgctg gcttgcttcc tggggcagaa
      361 agtgtttggt ctggataagc acaccagctg gttgatcggt gccggtagca gtatctgtgg
      421 tgctgccgcg gtactggcga ctgagccggt agtgaaagca gaagccagta aagtaaccgt
      481 ggctgttgca accgttgtta tcttcgggac cgtcgcgatt ttcctctacc cggcgatata
      541 tccgctgatg tctcaatggt ttagtccgga aactttcggt atctacattg gttctactgt
      601 gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc agcccggatg cagaaaacgc
      661 agcagttatt tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca tcctgctggc
      721 ggcgcgtgtt aaacagctgt ctgggacgaa cagcggcgag aaaagcaaaa tcactattcc
      781 gtggtttgcc atcttgttca tcgtcgttgc catctttaac tcgttccacc tgttaccgca
      841 gagcgtggtg aacatgctgg taacgctgga taccttcctg ctggcaatgg cgatggcggc
      901 gctgggtctg accactcacg tcagcgcgct gaaaaaagcc ggggcgaaac ctctgctgat
      961 ggcactggta ctgtttgcct ggctgattgt tggtggtggt gcgattaact atgtgattca
     1021 aagcgtcatc gcataaacca ctacatcttg ctcctgttaa cccgctatca ttaccgtttt
     1081 cctccagcgg gtttaacagg agtcctcgca tgaaatacat tggagcgcac gttagtgctg
     1141 ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct
     1201 tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg
     1261 aattcaaagc cgcctgtgaa aaatatcact acacatcggc gcaaattctt ccccacgaca
     1321 gttatctgat taacctcgga catccggttg ctgaagctct ggaaaaatcg cgcgatgcct
     1381 ttatagatga aatgcagcgt tgcgaacagc tggggctttc tttgctcaac ttccaccctg
     1441 gcagccatct gatgcagatt tcagaagagg attgccttgc gcgtattgcc gaatccatca
     1501 acattgcgct ggataaaact caaggtgtca ccgcggtgat tgaaaacacc gccggtcagg
     1561 gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc gtggaagata
     1621 aatcccgcgt cggcgtctgc attgatacct gccatgcttt cgctgccggg tatgatttgc
     1681 gtactcctgc cgaatgcgag aaaacgttcg ctgattttgc ccgtattgta ggctttaagt
     1741 atctgcgcgg gatgcacctc aacgatgcga aaagcacctt tggcagccgc gttgaccgtc
     1801 atcatagcct cggcgaaggc aatatcggtc atgatgcgtt ccgctggatc atgcaggacg
     1861 accgtttcga cggcattccg ctgatccttg aaaccatcaa cccggatatc tgggcagaag
     1921 agatcgcctg gctgaaagcg caacaaactg aaaaagcggt ggcctgatcg atgaacgaca
     1981 gggaaaaaca gattcttaaa attttacggc gtaacccgct gattcaacag aatgaaatcg
     2041 ccgatattct gcaaatcagc cgctcacgtg ttgccgctca tattatggat ttgatgcgca
     2101 agggtttgat taaaggcaaa ggatatatcc ttaccgagca ggattactgt gtggttgtgg
     2161 gtgcaatcaa tatggatatt cgcggaatgg cggatatccg ttacccgcaa gcagcctctc
     2221 atccaggttc tgttcactgt tccgcaggcg gcgtcggacg taatatcgcg cataacctgg
     2281 ctctgcttgg tcgggatgtg catttaattt cagctattgg caacgatttt tacggcgaga
     2341 cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc aaactgcatt cgtctgcatg
     2401 gacacagcac tgcaacttat ctcgctatcg ctaataagca ggaagaaact atcctggcaa
     2461 tcaatgacac acacattctg caacagctaa cgcctcaact gctcaatact tcacgggact
     2521 tgattcgcca tgctggtgtg gtgctggctg attgtaatct cacacctgaa gccctggagt
     2581 gggtctttac cattgctgat gaaattccga tgtttgtcga taccgtttcc gaatttaaag
     2641 cgaacaaagt aaaaaactgg tacagccgga tccacactct gaaaccaaca caaaatgaac
     2701 tggaaatttt gtgggggcaa ccgattaaag acgacaacga tcgcatcaga gctgtaaatt
     2761 cgctgcatca acaaggtgtt aaacgcattt ttgtttatct gaaagatgaa tctgtatttt
     2821 gcagcgataa ggatggagaa caatttttgc tgaccgcacc agcgcatacg acagtagaca
     2881 gttttggtgc tgacgatggt tttatggcgg gcctggtata tagctttctt gaaggataca
     2941 gtttccgcga cagcgcccgt tttgcgatag cctgcgcggc aatttcgcgt gccagcggca
     3001 gcttaaacaa ccctaccctg tctgccgata acgcgctttc attagtgcca atggtgtaac
     3061 aatgttgccg gattcgacgc ttgaccgcat cttatccggc tcataaattg cgcactacgc
     3121 taaaccaata aagaatcctg caatagtagc actcatcagg ttactgacag caaaaacgca
     3181 atagccagca ataccaccat tcccagaaca cttctcatga catccatagt tattatctct
     3241 tcatgtcatt ttgggcgacg gctctctgcc catcataaat agccgggcct gcatcttgcg
     3301 atactgaccc ggcttgttat caacacttaa tgggttttga tgtagccgcg tacgcactct
     3361 tcgactaatc cccagaacca gtctgtatca atagtgatgc cgactttggt attggcgggc
     3421 ttgccaagaa cgcccaactc gtcgcagacg gtacgcccat agcaagggcc actgttaacg
     3481 tccacttcga cgtacatctc ctgggtttta atgccatcag ggttaatcaa ataaccgatg
     3541 caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt tttcgaactg cgttttgaga
     3601 gtgaagttca tgatgtcgct gaacagctct cccgcagggc cgccagccct ttccatccga
     3661 gcaatcacgt ccggggtgca aacggtctgg ttggtaagat cgaggcccat catcactaat
     3721 ggaacgccgg aggtgaacac cacgcgtgcg gcttccgggt cagcaaagat attgaactca
     3781 gcagatgggg tgaagttgcc agtaccataa gcgccgccca tcagaacaat ttcacggatt
     3841 ttcggcagga tcgcgggttg catacgcatt gccaccgcga tgtttgacaa cggaccaacc
     3901 ggcaccagag tgatatcgcc atcgcttgcc ataagggtat cgatgatata tttcaccgca
     3961 tgagtgtttt ctgcctggcg ggtcagcggc tcgaataccg ggccatccag tccggtttca
     4021 ccgtgaatat tatcggcaac aatttgttga cgcataatgg gctgcggcat ccccgcataa
     4081 accggaacat taatctccag tttctggcaa acattcaggc cattaattaa tgttttatcg
     4141 agggtttgat tacccgctac aatggtgata cctaataaat caatcgcagg atgtttcgcc
     4201 gccatcatta tagcaatagc atcatcatga cccggatcac aatccagaat aatttttctc
     4261 ttttccattg tttatttcct ctgtttccag ttgcgttatt gtttctacag caaagaaaat
     4321 tcgacctggc cgatgattga atcttaacaa cagcgtacgt atgctaaata tgagaaatct
     4381 catagcggat aaacatcgtg aaagaaatcc acaataatga tcttaagcag caattgatga
     4441 gtgaatctgc gtttaaggat tgcttttcaa cggatgtttc agccgatacg cggctgtttc
     4501 attttttagc gcgtgactac attgtgcagg aagggcaaca gccgtcctgg ctgttttacc
     4561 tgacgcgagg ccgcgccagg ctttacgtca cgcttgctaa tggtcgcgtg tcgctgatcg
     4621 atttctttgc cgcgccctgt tttattggcg agattgagtt aatcgacaaa gaccatgaac
     4681 cgcgtgcagt gcaggctatt gaagagtgtt ggtgccttgc gctccctatg aagcattacc
     4741 gcccgctgtt attaaacgac acgctatttt tacgaaaact ctgcgtcacc ttaagtcata
     4801 aaaattatcg taatattgtt tctttaactc agaatcaatc atttccgtta gtgaatcgcc
     4861 tggcagcttt tatattactc tcgcaggaag gtgatcttta tcacgaaaag catacgcaag
     4921 cggcagagta tttaggcgtt tcttatcgac atcttttata tgttctcgcg caattcatta
     4981 atgacggttt attaattaaa agcaagaaag ggtatctcat taaaaacaga aagcagttgt
     5041 caggactggc gctggagatg gacccggaga ataaattctc cgggatgatg cagtaaaaat
     5101 tattggcaat agtgcgattg ccggatgcaa cgcttaataa ggcgtacatc cggcaatgaa
     5161 actcaagcta aaccaataaa gaacccggca atggtcgcgc tcatcaggtt ggaaagcgtc
     5221 gccgccgcca gcgcccgtaa accaagctgg gcgatttccg gcgcgcgttt tggcgaaata
     5281 gccgaaaacg ctccaacgac aacaccgata gaaccaaagt tagcaaaccc acagagggcg
     5341 aacgagataa tggcaatggt cttgacatcc agcgtgccgc tcgtttgcag atagggcgag
     5401 aaattgagat aagcaacaaa ctcattaatc gccaatttct gcccaatcaa acttccggca
     5461 agatttgcat cactccagtc cacccccata atccatgcca acggggccag cacatagccg
     5521 aaaatgcttt ccagagagac attggcaaaa ccaaaccagc cgccaactcc accaataatg
     5581 ccgttgatca gcgcaatgat cgccacaaat gccattacca ccgtcgccac accggcggcg
     5641 attttcagcc cggtcattgc accgttcgcc gcagcttcaa taatgctttt tggcggtgtt
     5701 tcgctaaacg acagattttc aaacgtaact tgcgaaggtt ccgtcgccgg actaagaata
     5761 cgtgcaaaca ggataccgcc aggaatagcc atcagcgaag cggctaacaa atagtcaatc
     5821 ggcacgccca ttccggcata gccaatcatc atcgaacccg caatagacgc catgccgcta
     5881 caaatagccg taaatagctc gttgcgattc atgcgatcaa taaaaggttt aacgatcgcc
     5941 gggatctcat tttgcccgag gaagatagtg gtgactgcga caaaagattc gattttgctg
     6001 atgttgaggg ctttctgaaa aatactgcca agaatacgaa tcagcagccc cattacgcca
     6061 atgtagtaca gcaagctgat aagcgcggta acgaaaataa tcgccgggag tacgcgaaag
     6121 gcgaagataa agcccgcacc gtcaaacaga acatccattt tgggaccgac cagcgaacca
     6181 aaaataaagg cacttcctgc gtcactgtag gacatcactt tatggacgcc taatgccgcc
     6241 tgctctactg cccattttcc cgggggaaaa tagagcatga tgccaccaat ggcaatttgc
     6301 agcagcagcg cagccccaac cgtgcgcaag ctgatgctct ttttatttac cgacaacaca
     6361 aaggcaattg ccagcaatac caccatcccc acaacacttc tcattatatc cataatgatt
     6421 ttcccttcat gccagtaaac ccggcgtcag cgccaggttt tggtatgttt gatgagtacg
     6481 ggcgacggct ttctgcccac cagaaattaa cccacgagac gctgatattc tttggcaatt
     6541 tcgctcgcca gaatggcgtt attaaagacc agttgaatat tggatttcag gctatcaccg
     6601 ccggttagct ccgcaacacg cgccagcagg aacggtgtac tctctttccc aataacaccc
     6661 tgttcttcgg cttcagcaac ggcttgatca attgctgcat tgatggtttc ttctggcatc
     6721 gcaaactgtt ccgggatcgg gttcgctacc accaggccgc cgttcaaacc ggtttgccac
     6781 ttcactgcca tcgcgcgagc aatttcgcgg gcactgtcga gacgaatgct gacatcaaat
     6841 gggctggttc ggcagaaaaa cgcaggcagc gcttttgtct gataaccaat taacggtaca
     6901 ccgaaggttt ctaaatattc tgttgttaat cctaaatcga gaatagattt tgcccccgca
     6961 caaacaacag tgacattggt atttgccagt tcctgtaaat cggcagaaat atcgaaggta
     7021 tgttccgctc cgcgatgcac accaccaata cctccggttg caaaaacttt aatcccagcc
     7081 agtgctgcaa taatcatggt tgatgcaacg gtagtcgcgc catttttccc tgcagcaacg
     7141 acaaaaggta aatcacgacg actaactttc gtcacgttat gtccttcacg gcccagcaat
     7201 tcgatttctt ctttacttaa gcccactttc atcacaccgc caataatggc gatagtggct
     7261 ggtactgcgc cctgtttacg aattgtttct tctacttcaa ttgcggtttg ggcattttgt
     7321 gggaatggca ttccgtgaga aattatggtc gattccagcg caacaaccgg ttttttattt
     7381 tttaaagcat cctgtacttc agcggatatt tgtaataatt caggggacag agttaattca
     7441 gacattctgt gttctccact aacgatgtaa cattggcaat tgataattca gggttgttgg
     7501 tatattcaca ggcaagtgcc attgacgaac atccctgcgc aaatcgaaca gaatcgataa
     7561 acggcatacc gtctacccaa caggaggcca gccccgccat catggcgtcg cctgccccag
     7621 taacattgat gacattcgtt ttaatagggg ccgaccaccc actttcaccg ttgatatcac
     7681 tgtagtaaac gccatcccct cccatgctca gcaccaggcg gttcaggccg tgttgatgaa
     7741 accaggcggc gacctttgcg acatcttcac gtccggaaag tgcaattccg ctcaacgttt
     7801 cggcttcaag acggttaggt tttaacgtat ggattttgct cagatgatcg cggattttga
     7861 cgcatttcca tgccgatacc ggatcaataa atacaggcgt ttccccgcta ttttccagaa
     7921 cccagaccag cgcctcttca ctaaggttgc aatccgcgac aataaccttt gcaccgcgaa
     7981 taaactcctg atgctgggca agaaattctg cagaaataca gtcactaata ctcatatcat
     8041 ttatcgcgac aagcatttct cctgtattat cgagcagtga taaatagctg gatgtattct
     8101 cacccggaac aattaagcat ttatcgacat aaacaccgga ttgattagtt tgcgccagta
     8161 atgattgccc ataaaaatca ccacctactg cacttaaaag ccaggcattt ttccccagta
     8221 acgccagatt atgagcgata ttccttccca cgccacctgg ggtaaattta atttttccgg
     8281 gattcgaatc cgcataattc aatggatgat gtgaataccc agccacatca atattcgccg
     8341 agccaataat tactatgtag tccttttcgc gcatagcacg ccccctggca ataaattgcc
     8401 aacgaagaaa ttgggtaaaa tagtcattac agtcaataag actattagag catgtgttca
     8461 gatttaaaca catgttcata ataagcatgt ttaacgtaat gtaaatcgat cagaaaaaac
     8521 gaaatcggcc agatatgaag gcggtcacaa aatttgtcta tttatgcagc aacaaaagag
     8581 aaagttgtga tgtgtaacac acaatatggc aggcaagaaa aagggcagag atgtttctgc
     8641 cctcagattg ttgacaatgc gcgctttgtt catgctggat gtggcgtaaa cgccttatcc
     8701 gacctacaaa agcgcaaaaa ttcaatatat tgcagggttc atgtaggtct gataagcata
     8761 gcgcatcagg caattttgcg tttgtcatca tcggggcaga gatgtctcat ccccgatgat
     8821 gaagagaaat tatgcgtttt tttctactat ttgtgtttcc gggcgtttga ggaaggcata
     8881 ggccaaaccc gccaccagcg taccggcaat aattgctacc aggtaaccca ataccggcgt
     8941 aatagcgcca gggatcagca gaacaaacag accaccgtgc ggagccatca gtttcgcacc
     9001 aatcgccatt gagattgcgc cagtcagcgc cccaccaacg atacagcacg gtagcacacg
     9061 catcggatca cgagcagcaa acggaattgc accttccgaa ataaagcaca gaccaagtac
     9121 cagagcggct ttgccacctt cctgctgcgc tttatcgaat ttacgacgcg ccaccattgt
     9181 tgccagaccc attgccagcg gtggcaccat acctgccgcc ataatcgccg ccatcgggcc
     9241 ataggtttga gtactcagca gacccacacc gaatgcgtac gctgctttgt ttaccggacc
     9301 gcccatgtca gtacacatca tgccaccgag gatcgccccc agcagaaccg cattcgcagt
     9361 ccccatggtc tgcagccagt gagtcaaccc atcgagaatg ccagcaaccg gtttaccgat
     9421 taggtagatc atcgccagac cgaccaccag actggaaatt agcggaatga tcaggatcgg
     9481 tttcagtgcc tccatactct gtggcagttt cagttgcgta ctgattaact tcgcaatgta
     9541 acccgccagg aagcccgcaa taataccgcc aatgaagcca gaaccggtgc taaccgccag
     9601 cataccgcca atcagacccg gcgtgagacc cggacgatcg gcaatggaga aggcaatata
     9661 acctgccagt accggcacca tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc
     9721 cgctgccaac gtacccggct ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat
     9781 acacagacca cctgcaacaa ccatcggcag catgtaagag acgcccgtca gcaagtgacg
     9841 gtaagcgcct gcactctctt tcttaccttc agtggtcgct gtttgagctt tgcccgctgg
     9901 ttcatacggc gttgcttcag caaccgcttt atccagttcc tgcgcagttt tcttcagcgc
     9961 cagaccggta gaggtgcgat acatcggttt accagcaaat ttcgccagat ccacttcgat
    10021 atctgccgcc acaatcacca gatccgctgc tgcgacttct tcaggtgtga ttgcattacc
    10081 cgcgccaaca gaaccacggg tttcaacttt cacccaccag ccacgttttt tcgcttcggt
    10141 ttcaatggct tcagccgcca taaaggtgtg tgctacacca gtcgggcaag cagtcaccgc
    10201 aactacgcgt ttcggaccgc tagcagcaac cggtgctgtc gcagcgaccg gcgcagtgta
    10261 aggtttcgca tggcctttgg cttcactcag gaacagctca gggtgcgcaa ctgcccggga
    10321 aatatcgccc agccagacat ttttaccgtt cagcgcgctg tcattcggga tgaaatcacc
    10381 gagaacaatc gccatttctg cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg
    10441 cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt gcctgaccga gattagcgtc
    10501 aataatcagc agcgttttca ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga
    10561 cgcgcgccat cattgcggcc aactgcggac gatcggtaat acccacattg ctttgactta
    10621 ccgccagagc tgcaacagct gtcgccagac gcagtgtgtg ttcactggat tcacgcatca
    10681 gcaagccata aatcaggcca ccaaccatag aatcccctgc gccaacggtg cttacgacat
    10741 cgactgacgg tggtttggcg atccattcgc cggaggcatt aacccaaagc gcgccttcgg
    10801 cacccagtga aataacaaca tgcgcaatac cttgttcacg tagcgcatgt gcagcttcaa
    10861 tcacatcttt catttcaggc agtttacggc ctgcccagat ttccagctcg cggcggttag
    10921 gtttcaccag ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg ctactatcaa
    10981 agataatgca aggacactga ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc
    11041 tgacgcctga cggtaagctt ccgctgacac agaccatatc gaactgaccg agccagctca
    11101 gagaatcagt cacaaagcgt tcccagtcgg cgggggtaac ttcaaaaccc gagaagttga
    11161 agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt aattcgggtg cgcccctgta
    11221 caacctggaa acggttggca atgcccagct cgctgaacag ttgctgaaaa ccatcctgat
    11281 tgtctttacc caggaagccg ccaacggtga catcaattcc caggtctttt aatactttgg
    11341 ccacgttgat gcctttaccc gccgcatgca gaccggtggt tttcaccagg ttcacttcgc
    11401 cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc cggattaagg gtgatcgtag
    11461 caacacgtct gctcattatg cgccctcccc aagaccagca gcgatagcgt cgccaatggc
    11521 tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt
    11581 aacgccaagt gccacaactt tcatcagact acgtccgttt gccggtttgc cggtgccatc
    11641 aaggtttgtc acggtaatat cactgttaaa ttgtttaatg gtattgacca gcatggtacc
    11701 tggacgagca tgcaggccgt gttcattgcg caccacaaac tccgcgctta acacgtcgtc
    11761 ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa
    11821 gcggtcagct ttattatcga gcaacaaatc agcgagacgc ttaagaaccg cgatgggctg
    11881 atcgtcattc attgccacac tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc
    11941 atttgccgca cggcttaccg caatcgcgct acgcagattg ccttcggcgc tatcgctcag
    12001 ccagataccc tgtccgaggt tcagcggttg ttcattgatg gctttggtga cgaaagtggc
    12061 gtcaactgcc cccgcttctt tcagacgcgc agcgttcagc gcctgaagag tcagcagatc
    12121 gctggcgacg atatccagcg tcagcatttc gttgtcgagc ttcagctgct cactctgctt
    12181 ttcgcccatc agcaatgcgc gaagttcttc tgctgttgtt gctgacttca gttgttcagc
    12241 aacggaatca tcgctcagta cgtgggtcag ctggcgtagc aggcccagat gttcatccga
    12301 gctggcagca ataccgatcg ccacgtacgc tacctggccg tccccccagg tgacgccttc
    12361 cgggaactga aatacctgaa cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc
    12421 gtgtggaata gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat
    12481 gccattgacg tagccttcag ctacattacc ggcctgcacc agcgccgcag cgacctggcg
    12541 aatcgcctct tctttgtctc cggccttttc gcccggatgg atgtcctgta cagataactg
    12601 gaacatagtt ctcctctctt gctgaattga aacgattcag cttctatgag aaaaaaagcg
    12661 ccaacctggc ttagggttaa agacaagatc gcgctgaaac gtttcaagga agcataatac
    12721 ttctgtttca gcgcgcaagg aaagccgtaa agttatgagc aaaagtttga tgtgctgcac
    12781 aattttttgt catttttcct aattgctgat gggaaaatcc gttatcagca atttcatttc
    12841 agcagtgtca gcagccaatc agcaacagtg aaatggcatt ttgattattc gtggcgcttt
    12901 tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca
    12961 gtgatcgacc agcatgcata actcccccac agtcaccagc gcgaaatcgt ttgacctgac
    13021 ctcgacggcg tttttaatcg ttgcctttct caccggtatt gcgggcgctc tgcaaacccc
    13081 gacactcagt atttttctta ctgatgaagt acatgcccgt ccggcgatgg tgggattctt
    13141 ctttaccggc agcgctgtca ttgggattct ggtgagtcag tttctcgccg ggcgctctga
    13201 taagcgcggc gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg tgctggcctg
    13261 cacccttttt gcctggaatc gcaactactt tgttttgcta ttcgttggcg tctttcttag
    13321 cagctttggc tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac atgctgacaa
    13381 aaccggacgt gaggcggtga tgttcagctc ttttttacgc gctcaggttt cactggcatg
    13441 ggtcattggc ccaccgctgg cttatgcctt agcgatgggt ttcagcttta cggtaatgta
    13501 tctgagcgca gcggtagcgt ttattgtttg cggcgtgatg gtgtggctgt ttttgccgtc
    13561 gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg
    13621 cgatacgttg ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca gcctgtacat
    13681 catcaacatg ccgctattta ttatcaacga actgcatctt cccgagaaac tggccggtgt
    13741 gatgatgggg accgccgccg ggctggaaat cccgaccatg ttgatcgccg gatattttgc
    13801 caaacgtctg ggtaagcgct tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta
    13861 cgcaggaatg ctgatggcgc attcacctgt cattctgttg ggcttgcagc tgctaaatgc
    13921 tatttttatt ggcattcttg gcggcatcgg gatgctctat tttcaggatt tgatgcccgg
    13981 tcaggcaggt tcagccacca cgctctatac caacacttcg cgcgtgggct ggatcatcgc
    14041 aggatcagtg gcgggcatcg tcgccgagat ctggaattat cacgctgtgt tctggtttgc
    14101 gatggtgatg attatcgcca ctctgttttg cttactgcga attaaagatg tttaaggcgc
    14161 ggtcaacatt tccagatcga taagccagat catggcctgc tgacgcgagt taccgcacat
    14221 ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt
    14281 acagcgttgc tgttcatcaa gctgaatgca gggagtattg gcgggcttgc catctggcat
    14341 accgggaata gggctggaaa ttgaaggggc agtgcaacac gccccgcaac ccggacggca
    14401 ttccatacgt tgttctctta aggttaacca ccggattgat gagcgcgcac agtaccacct
    14461 tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg
    14521 tcgcgatatt tttgacattt ttgactacag gaatttttcg atgccaagag cgaacgaaat
    14581 taaaaaaggt atggtactga attacaacgg caaactgctg ctggttaagg acattgatat
    14641 tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg
    14701 taccgggctg aaagtagaag agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct
    14761 gacccgccgt tacgttgact tctcctatgt cgatggcaac gaatatgtct ttatggataa
    14821 agaagactat accccgtata ccttcaccaa agatcagatt gaagaagagt tgctgtttat
    14881 gcctgagggc ggcatgccgg acatgcaggt gctgacctgg gacggtcagt tgctggcgct
    14941 tgagctgccg cagaccgttg atctggaaat cgttgaaact gcaccaggta tcaaaggggc
    15001 atctgccagc gcccgtaaca aaccggcgac attgagcact ggtctggtga ttcaggtacc
    15061 agaatactta agcccgggcg aaaaaattcg tatccatatc gaagaacgcc gttatatggg
    15121 tcgtgctgac taacttcagc cgcatgcaga aaagggatag ctcaggctgt ccctttttta
    15181 atttattata ccaatcttct attttgcgct tcctgaacac cttacgcccg caattttcac
    15241 tcgttgattg atcgccctca cacttcatcg cattaacaat ccagaccaat ttcaattgct
    15301 gtcatataac tttacactgt cattgttaat taatcgttac taagacgtga ctcctatgaa
    15361 tacaatcgcc tccgttacgc tcccgcatca tgtacacgct ccacgctacg atcgacagca
    15421 gttgcaatca cgtatcgttc attttggctt tggagccttt caccgcgcac atcaggcatt
    15481 actgaccgat cgtgtgctga atgcccaggg cggcgactgg gggatctgtg aaatcagctt
    15541 gttcagcggc gatcaactga tgagccagct ccgcgcacag aaccatttat ataccgtgct
    15601 ggagaaaggt gcggacggca atcaggcgat aattgtcggt gccgttcacg aatgtctgaa
    15661 tgcaaaactg gattccttag cggcaattat tgagaaattt tgcgagccac aggtggcaat
    15721 tgtttccctg acaattaccg aaaaaggcta ttgtattgac ccggccaccg gtgcactcga
    15781 caccagcaat ccgcgtatta ttcacgatct acaaaaccct gaagaacctc actccgcgcc
    15841 aggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc ggccatacac cgtttaccgt
    15901 gctctcctgc gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat
    15961 ggcagaaaaa cgttcgccag aactcgccgg gtggataaaa gaacacgtca gttttccggg
    16021 aaccatggtc gaccgcattg ttccggctgc aacaaacgaa tcactggcgg aaataagcca
    16081 gcatctgggg gtgaatgatc cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt
    16141 tgaagataac ttcgtcgctg ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa
    16201 tgatgtcctg ccatgggaag agatgaaact gcggatgctt aatggcagcc actcttttct
    16261 cgcttatctg ggttacctct caggattcgc ccatatcagt gattgcatgc aggatcgcgc
    16321 atttcgccat gccgccagaa cattaatgct ggatgagcaa gcgccgacac tgcggattaa
    16381 agatgtcgat ttaacacaat atgcggataa gttaattgca cgttttgcta atccggcact
    16441 gaaacataag acctggcaaa tcgcgatgga tggcagccag aaattaccgc aacgcatgtt
    16501 ggcaggtatt cgcatacatc tggggcgcga aacggactgg tcgttgctgg cattaggcgt
    16561 tgcaggctgg atgcgttacg tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg
    16621 cgatccgctt agcgataaaa ttcgtgaact tgttgcaggc agcagcagtg aacaacgcgt
    16681 aaccgccctg ctttccctgc gtgaaatttt cggtgatgat ctgccagata acccgcattt
    16741 tgtgcaggtc atcgaacaag cctggcaaca aatcgcacaa ttcggcgcgc atcaggcgct
    16801 attaaacacc ctcaaaattt aacgatttct gcggttaaag cggatgaagc tcgccttcgt
    16861 ccgctctccc cttctctttt ctgccttttt tagccaggat taacgctcag ttaacttacc
    16921 agagtattgc ggagccgttg tgaccagaac caacctcatc accggttttc tcggcagcgg
    16981 gaaaactacg tcgattcttc atctgttagc ccataaagat cccaacgaaa aatgggcggt
    17041 actggttaat gaatttgggg aagtcggaat tgatggtgct ttgctcgccg atagcggcgc
    17101 actgctgaaa gagatccccg gcggttgcat gtgctgcgtt aatggtttac ccatgcaggt
    17161 agggttgaat accttactgc gtcagggaaa accagaccgc ttgttgatag agccgaccgg
    17221 gctgggccat ccgaaacaga tcctcgatct cttaaccgcg ccagtctatg aaccgtggat
    17281 agatctgcgc gccacgttgt gcattctcga tccacgcctg ctgctggacg aaaaaagcgc
    17341 cagcaatgaa aacttccgtg accagctggc tgccgcagac attattgtcg ccaataaatc
    17401 cgaccgtgcg acgcccgaaa gtgagcaagc gctagagcgc tggtggcagc aaaatggtgg
    17461 cgatcggcaa ttaattcaca gcgagcatgg aaaaattgac ggtcatcttc tggatttgcc
    17521 gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat tctcatcagc atagcgtgaa
    17581 aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag
    17641 cggacaaggg catcaggcct gtggctggat attcgacgct gatacggtat tcgacaccat
    17701 tggtattctg gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat
    17761 tcccgaaggg ctggtgcgaa tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa
    17821 cgttgcgcca ccggacagtc gtattgagct gatttccagc agcgaagctg actggaatgc
    17881 cttgcagagc gcgctgttga agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt
    17941 ttcagtaaga taattagaga aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat
    18001 attgtcggcc tcgcgctgtt tctttcctgg tatattccca ttaatcatgg attctggttg
    18061 ccgattgatg cggatatttt ttatttcttt aatcagaaac tggtcgaaag taaggccttt
    18121 ttgtggctgg ttgcattgac caacaatcgc gctttcgacg gttgttcact gctggcgatg
    18181 ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc caggcagacg acgtatcgtg
    18241 attattggtc tggtcatgct attaactgca gtggtattaa accagctggg tcaggcatta
    18301 attcctgtaa aacgggccag tccaacgttg acttttacca atattaaccg cgtcagcgaa
    18361 ctgctctctg ttcccacgaa agatgcctca cgagatagct ttcccggcga tcacggtatg
    18421 atgctgctta ttttttcggc attcatgtgg cgttatttcg gcaaagttgc tggccttatc
    18481 gcccttatta tttttgtggt ttttgcgttt cccagagtaa tgattggcgc acactggttt
    18541 actgacatca tcgtcggttc catgaccgtg atattgatcg gtttgccctg ggtgttgctg
    18601 acgccattaa gtgatcgatt aatcaccttt tttgacaaat cactacctgg aaaaaacaaa
    18661 catttccaaa acaaataact cacagtaatt aacatcatca ggtttatttt tatagtgagg
    18721 ataatcctga tgatgcgcct cctgctttca tccatcgagc gcaaaaatca ttctctaagt
    18781 aaatgaatgg attgcatgcg tttcactcaa tcgtacttta attgaccaat cccgctgatt
    18841 aactttctgt atcacttttt cttataaaaa atcatgtaaa accgctcgcc aagaccgcac
    18901 caatcgggta atctcgaact cgttttgcct cggcggtaga ttatcctcac agcatataat
    18961 tttgtgcgtt agtccacaga tttggcctta aggaattgtt tcaacatgcc caggtaatta
    19021 gtctcgtgtc gcttggcatt tttttataac gatatttgtc gttaaggact tcaagggaaa
    19081 acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg gatccccgcg
    19141 attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat
    19201 cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca ggatgaattt
    19261 gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg
    19321 aaaggcgtac gctatcgtct gggcggcagc actaaaaaag gtatcgattg ttctggtttc
    19381 gtacagcgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac ttatgaacag
    19441 caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt agttctgttc
    19501 cgtgccggtt caacgggacg ccatgtcggt atttatatcg gcaacaacca gtttgtccat
    19561 gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccatactg gaagaagcgt
    19621 tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat gcaatccctt
    19681 ggctatcctg acgagttaac tgaaagcact gcttaggcag tgcttttttg ttttcattca
    19741 tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat tgttatcttt
    19801 taaatgttgt cgtaatttca ggaaattaac ggaatcatgt ttatacgcgc tcccaattct
    19861 ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtga tgattgcgat actggtgagc
    19921 tgccttcagt ttttagtggc ctggcataag cacgaagtca aatacgacac actgattacc
    19981 gacgtacaaa agtatctcga tacctatttt gccgacctta aatccactac tgaccggctc
    20041 cagccgctga ccttagatac ctgccagcag gctaaccccg aactgaccgc ccgcgcggcg
    20101 tttagcatga atgttcgtac gtttgtgctg gtaaaagata aaaaaacatt ctgttcatct
    20161 gcaactggtg agatggacat tccactcaat gaattgattc cggcgctcga cattaataaa
    20221 aacgtcgata tggcgatctt acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc
    20281 atctggtatc gcaacccttt gctgaaaaat agcggcgtgt ttgccgctct gaatctcaac
    20341 ctgacgcctt cactctttta tagttcacgg caggaagatt acgatggcct cgccctcatt
    20401 attggtaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa tgaattaacc
    20461 gacatgccgg tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg gctttatgca
    20521 gatgactgga catggaacga tgtgtggtac gcatttttac tgggtggcat gagtggaact
    20581 ttcgttggac ttctctgcta ttacctgatg agtgtgcgta tgcgcccagg cagagaaatc
    20641 atgaccgcca tcaagcgcga acaattttac gtggtatatc aaccggtggt tgatacacaa
    20701 gctttgcggg taacgggcct ggaagtactg ctacgctggc ggcatcctgt agcaggagaa
    20761 atacccccgg atgccttcat taactttgcc gaagcgcaaa agatgattgt gccactgact
    20821 cagcacctgt ttgagttgat tgcccgcgat gccgcagaat tagaaaaagt actgccggta
    20881 ggcgtcaaat ttggcattaa cattgcgccg gcccacttgc acagcgaaag ctttaaagcg
    20941 gatatccaga aactgctcac ttccctgccc gcacaccatt tccagattgt gctggaaatt
    21001 accgagcgcg atatgctgaa agagcgagaa gccacacaac tcttcgcctg gctgcattcg
    21061 gtcggcgtag aaatcgctat tgatgacttc ggcaccgggc acagcgcgct tatctatctt
    21121 gagcgtttta cgctcgatta tctgaaaatt gatcgtggat ttatcaacgc catcggtacg
    21181 gaaacgatca cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg tctcaatatg
    21241 ctgacagttg ctgaaggggt cgaaacgcca gaacaggcac gatggctaag cgaacgcggc
    21301 gttaatttca tgcaaggcta ctggattagt cgcccgttac cgctggacga ttttgttcgc
    21361 tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct tattattcat
    21421 agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccgtt gcagatgatt
    21481 gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgcgcaggc gcaggctatc
    21541 aaggaaagct atgcctttgc agtactgggc gaaccccggt acgcatttaa tttcaaccat
    21601 tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaaa taacgttgtc tgccctcggc
    21661 accttcgata atttcaaccg ctatgcactg cgcggcaatc cgggcgcacg caccgagcag
    21721 ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta cccgctgatt
    21781 gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat taatccacgc
    21841 gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac ttttcaaaaa
    21901 tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc
    21961 attgcgccgt taaccgtgcg cattgagtta gctaaacccg gcaaagaaga tatgctgagt
    22021 ctgttttcgc tgccggtatt tccagaaaag tactggaaag atcacaaact tagcgacccg
    22081 ctcgccacgc ctccgcttgc cagtggtccg taccgcatta cgtcctggaa aatggggcaa
    22141 aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt aaaccgtgga
    22201 cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt cgcctttgaa
    22261 gcgtttaaag caggagcctt tgatttgcgt atggaaaacg acgccaaaaa ctgggccacg
    22321 cgctataccg gtaaaaattt cgataaaaaa tacatcatca aagatgagca aaagaacgaa
    22381 tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccggtatt cagcgatcgc
    22441 cgggtgcggg aagcgatcac tctcgccttt gactttgaat ggatgaacaa ggcgttgttt
    22501 tacaatacct ggagtcgcac gaacagttat tttcagaata ccgaatatgc ggccagaaat
    22561 taccccgacg ccgcggaact ggtgcttctg gcaccaatga aaaaagatct accgccagaa
    22621 gtcttcacgc aaatctacca gccgccggta tccaaaggcg atggctacga tcgtgacaac
    22681 ctgttaaaag ccgacaaact tctcaacgaa gcaggctggg tgctgaaggg tcagcaacgc
    22741 gttaatgcca caacaggtca gccactcagc tttgaattat tgcttcccgc aagcagcaat
    22801 agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa catggacatt
    22861 cgcaaggtgg ataactcgca aatcaccaac cgcatgcgca gtcgcgacta tgacatgatg
    22921 ccgcgcgtat ggcgggcgat gccgtggccc agttccgatt tacagatttc ctggtcatcg
    22981 gaatatatca attccactta taatgccccc ggcgtgcaaa gcccggttat cgactcgctg
    23041 atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt gggacgagca
    23101 ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat ggcggaagac
    23161 cgtctcgcct ggtgggataa attctcccaa cccgctgtac gcccagttta cagcctgggt
    23221 attgatacct ggtggtatga cgttaataaa gcgacgaaac tgccgtcagc cagacaacag
    23281 ggagagtaga tgggcgctta tctgatccgc cgtctgttgc tggtgatccc aacgctatgg
    23341 gcgattatca ctatcaactt tttcatcgtg caaattgcgc ctggcggtcc ggtcgaccag
    23401 gccatcgccg ccattgagtt tggcaatgcc ggagtattac ccggcgcagg cggtgaaggt
    23461 gttcgcgcca gccatgcgca aacgggcgtc ggcaatatca gcgacagtaa ttaccgtggc
    23521 ggacgcggat tagatccaga agtgatcgcc gagatcactc atcgctacgg tttcgataag
    23581 ccgatccacg aacgttactt caaaatgctc tgggactaca tccgttttga ttttggcgac
    23641 agcctttttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc ggtctccatc
    23701 accctcggat tgtggagcac gctgattatc tatctggtgt cgattccgtt aggcattcgc
    23761 aaagctgttt ataatgggag ccgttttgac gtctggagta gtgcatttat catcatcggc
    23821 tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc gggcggcagc
    23881 tatttcgatc tgttccctct gcgcggcctg gtttccgcta actttgattc gctgccgtgg
    23941 tatcagaaaa tcaccgatta tctgtggcat atcacgctgc cggtgctggc gacagtgatt
    24001 ggcggctttg cggctctgac catgctgaca aaaaactcat tccttgatga agtgcgtaag
    24061 caatacgtgg tgacggcacg tgcgaaaggg gtaagtgaaa aaaatattct ctggaaacat
    24121 gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt tatcagcatg
    24181 ttttttaccg gctcgctgct gattgaggtg atgttttcac tcaatggtct tggcttactg
    24241 ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt gtatattttc
    24301 accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct ggtcgatccg
    24361 cgtatagact ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag gcccgttggg
    24421 cgcgttttcg ccataaccgt cgcggctact ggtcgttatg gattttcctc ctcttgtttg
    24481 gtttgagttt gtgttctgaa cttatcgcca acgataaacc gttgctggtg cgttatgacg
    24541 gcagttggta tttcccgttg ttgaaaaact acagcgaaag cgattttggc ggcccgctgg
    24601 caagtcaggc tgattatcag gacccgtggc tgaaacaacg actggaaaat aacggctggg
    24661 tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc gataagccct
    24721 tcccttctcc accctcccgg caaaactggc tgggaacgga tgccaatggc ggcgatgtgc
    24781 tggcgcgcat tctctatggc acgcggatct cagttctgtt tggcctgatg ctgaccctct
    24841 gctccagcgt gatgggtgtg ctggcggggg cgctacaagg ctattacggt ggtaaagtcg
    24901 atctctgggg gcaacgtttt attgaagtat ggtcggggat gccgacgctg tttttgatta
    24961 ttttactttc cagcgtcgta cagcctaact tctggtggct gctggcaatt actgtcttgt
    25021 ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtacc cgtaatttcg
    25081 actacatccg cgcggcgcag gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata
    25141 tgttgccaaa tgccatggtc gctaccctca cttttttacc gtttatttta tgtagttcga
    25201 taaccaccct gacctcactc gatttcctcg gcttcggtct gccgctcggt tcaccgtcac
    25261 tcggcgaact cctgttacaa gggaaaaata accttcaggc cccgtggctt gggatcaccg
    25321 ccttcttatc ggtggcgata ttgttgtctt tgctgatctt tattggtgaa gccgtccgcg
    25381 acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat
    25441 ttgtcggtgg gttttcgcca tcagcaaact gttcgtaccg tagtgaatga tgtgtcacta
    25501 cacattgagg ctggcgaaac gctggcgctg gtaggtgagt ctggttcagg taagagcgtt
    25561 accgcgctgt caattttacg cctgctccct tgcccgccgg ttgaatatct ctccggcgat
    25621 attcgttttc atggcgaatc gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc
    25681 ggtaataaga tcgccatgat ttttcaggag ccgatggtgt cgttaaatcc attacatacc
    25741 ctggaaaaac agctttatga agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct
    25801 cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc gccaggcggc aaaacggctg
    25861 acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat tgcgatggcg
    25921 ctgttaacgc gaccggaatt attaattgcc gatgaaccaa ccaccgcgct ggacgtctct
    25981 gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagctgaa tatgggtatg
    26041 ctgtttatta ctcataacct cagcattgtt agaaaactgg cccaccgcgt ggcggtaatg
    26101 caaaacggtc gctgtgtcga gaaaaataac gccgctacgc tatttgcctc acccactcat
    26161 ccttacacac aaaagctact caacagtgaa ccatctggcg acccggtgcc attgccagaa
    26221 cctgcctcaa cgttgctgga tgttgaacaa cttcaggttg ccttccccat tcgcaaaggg
    26281 attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt tacgctacgg
    26341 gcgggtgaaa cactgggttt agtgggcgag tccggttccg ggaaaagcac gacgggactg
    26401 gcgctgctgc gactgattaa ctctcatggc agcatcgtct ttgacggtca gccactgcaa
    26461 aatttaaatc gccgccagct gttacctatt cgtcatcgca ttcaggtggt atttcaggat
    26521 ccaaactcct cactcaaccc acgactcaac gttttgcaaa ttattgagga aggcttacgg
    26581 gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat
    26641 gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc tggtggtcag
    26701 cggcaacgta ttgcgattgc cagggcgtta attcttaagc cctcgctgat cattcttgat
    26761 gaaccaacat catcactcga caaaacggtg caggcgcaaa tattgacgct attgaaatca
    26821 ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca cgttgtccgc
    26881 gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca aggaccgtgc
    26941 gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc agctactggc gttgagctga
    27001 cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa
    27061 tcggcatgtt gcagcaaatt cgtcctggcg atttacaaac aggcacggct caccttcaca
    27121 ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt tgtacatcac
    27181 cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc tgttgtcacc
    27241 gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag cagaccagcg
    27301 atagttctgc ggcagtcgat ggacgattga gagttgtaat gaagtcactt attttccccg
    27361 gaagtacatt ttacttcaca atttatttac atcaatttta acacatcatc aacaatccgt
    27421 ctttatacag atcgtaaaga ttgatgcctc gcctgcgtgg cctatatggc tctgatttaa
    27481 ataatatctg cgggcgtggt cacgtttgcg gggctatatg tacccgtttc tgcgatctaa
    27541 ctcaaccttt tta
//
LOCUS       DAJOTB010000055        26954 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJOTB010000055 DAJOTB010000000
VERSION     DAJOTB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.055"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..442
                     /gene="cmtB"
                     /locus_tag="OHN28_002264"
     CDS             <1..442
                     /gene="cmtB"
                     /locus_tag="OHN28_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCQ2297660.1"
                     /translation="RLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQS
                     IKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIGL
                     SAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            470..1858
                     /gene="cmtA"
                     /locus_tag="OHN28_002265"
     CDS             470..1858
                     /gene="cmtA"
                     /locus_tag="OHN28_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCQ2297661.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1873..3150
                     /locus_tag="OHN28_002266"
     CDS             1873..3150
                     /locus_tag="OHN28_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2297662.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3147..4112
                     /gene="yggF"
                     /locus_tag="OHN28_002267"
     CDS             3147..4112
                     /gene="yggF"
                     /locus_tag="OHN28_002267"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2297663.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4134..4643
                     /gene="fumE"
                     /locus_tag="OHN28_002268"
     CDS             4134..4643
                     /gene="fumE"
                     /locus_tag="OHN28_002268"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCQ2297664.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4640..5353
                     /locus_tag="OHN28_002269"
     CDS             4640..5353
                     /locus_tag="OHN28_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCQ2297665.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5325..6002)
                     /locus_tag="OHN28_002270"
     CDS             complement(5325..6002)
                     /locus_tag="OHN28_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2297666.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5996..6673)
                     /locus_tag="OHN28_002271"
     CDS             complement(5996..6673)
                     /locus_tag="OHN28_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCQ2297667.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6661..7368)
                     /locus_tag="OHN28_002272"
     CDS             complement(6661..7368)
                     /locus_tag="OHN28_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCQ2297668.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7369..7947)
                     /locus_tag="OHN28_002273"
     CDS             complement(7369..7947)
                     /locus_tag="OHN28_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCQ2297669.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7970..8401)
                     /locus_tag="OHN28_002274"
     CDS             complement(7970..8401)
                     /locus_tag="OHN28_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2297670.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8773..9792
                     /gene="epd"
                     /locus_tag="OHN28_002275"
     CDS             8773..9792
                     /gene="epd"
                     /locus_tag="OHN28_002275"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCQ2297671.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9842..11005
                     /gene="pgk"
                     /locus_tag="OHN28_002276"
     CDS             9842..11005
                     /gene="pgk"
                     /locus_tag="OHN28_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCQ2297672.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAVLEERAKK"
     gene            11219..12298
                     /gene="fbaA"
                     /locus_tag="OHN28_002277"
     CDS             11219..12298
                     /gene="fbaA"
                     /locus_tag="OHN28_002277"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCQ2297673.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12489..13349
                     /gene="mscS"
                     /locus_tag="OHN28_002278"
     CDS             12489..13349
                     /gene="mscS"
                     /locus_tag="OHN28_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCQ2297674.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13488..14123
                     /gene="argO"
                     /locus_tag="OHN28_002279"
     CDS             13488..14123
                     /gene="argO"
                     /locus_tag="OHN28_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCQ2297675.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14216..14956
                     /locus_tag="OHN28_002280"
     CDS             14216..14956
                     /locus_tag="OHN28_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCQ2297676.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15124..16020
                     /locus_tag="OHN28_002281"
                     /pseudo
     CDS             15124..16020
                     /locus_tag="OHN28_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16017..17072)
                     /locus_tag="OHN28_002282"
     CDS             complement(16017..17072)
                     /locus_tag="OHN28_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCQ2297677.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(17275..18168)
                     /gene="argP"
                     /locus_tag="OHN28_002283"
     CDS             complement(17275..18168)
                     /gene="argP"
                     /locus_tag="OHN28_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCQ2297678.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18309..18539
                     /locus_tag="OHN28_002284"
     CDS             18309..18539
                     /locus_tag="OHN28_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297679.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18595..19254
                     /gene="rpiA"
                     /locus_tag="OHN28_002285"
     CDS             18595..19254
                     /gene="rpiA"
                     /locus_tag="OHN28_002285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCQ2297680.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19510..20742
                     /gene="serA"
                     /locus_tag="OHN28_002286"
     CDS             19510..20742
                     /gene="serA"
                     /locus_tag="OHN28_002286"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCQ2297681.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            20971..21030
                     /gene="ibsC"
                     /locus_tag="OHN28_002287"
     CDS             20971..21030
                     /gene="ibsC"
                     /locus_tag="OHN28_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCQ2297682.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21131..21733)
                     /locus_tag="OHN28_002288"
     CDS             complement(21131..21733)
                     /locus_tag="OHN28_002288"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCQ2297683.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21754..21937)
                     /gene="ssrS"
                     /locus_tag="OHN28_002289"
     ncRNA           complement(21754..21937)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OHN28_002289"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21979..22308)
                     /gene="zapA"
                     /locus_tag="OHN28_002290"
     CDS             complement(21979..22308)
                     /gene="zapA"
                     /locus_tag="OHN28_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCQ2297684.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22476..23054
                     /gene="ygfB"
                     /locus_tag="OHN28_002291"
     CDS             22476..23054
                     /gene="ygfB"
                     /locus_tag="OHN28_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCQ2297685.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23080..24405
                     /gene="pepP"
                     /locus_tag="OHN28_002292"
     CDS             23080..24405
                     /gene="pepP"
                     /locus_tag="OHN28_002292"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCQ2297686.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24402..25580
                     /gene="ubiH"
                     /locus_tag="OHN28_002293"
                     /gene_synonym="visB"
     CDS             24402..25580
                     /gene="ubiH"
                     /locus_tag="OHN28_002293"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCQ2297687.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25603..26805
                     /gene="ubiI"
                     /locus_tag="OHN28_002294"
                     /gene_synonym="visC"
     CDS             25603..26805
                     /gene="ubiI"
                     /locus_tag="OHN28_002294"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCQ2297688.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(26879..>26954)
                     /locus_tag="OHN28_002295"
     CDS             complement(26879..>26954)
                     /locus_tag="OHN28_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297689.1"
                     /translation="NTKSKIRGIRKYNAKSQVKLVYFK"
BASE COUNT         6513 a         6561 c         7276 g         6604 t
ORIGIN      
        1 gcggcttatt gattattttc cagaatcatc aatctcagtt atacattcag caaaagactg
       61 gcaggaagct atcgatttct cgatggcatc attgctggat aaaaactata tcagcgagaa
      121 ttacattcaa tctattaaag actccaccat cagcaatggt ccttattata ttctcgcacc
      181 aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt
      241 gacgttactt aaacaaggcg tttatttccc ggggaaggac gaaccgatta aattactcat
      301 cgggctttct gctgccgatg ccaattcgca cattggcgct attcaggcgt taagtgaatt
      361 actgtgcgaa gaagaaatac tcgaacaact cttaacagca tcatcagaaa aacaattagc
      421 ggacattatc agccgcggat aattactctc tcctctacag gataatatta tggaaaacaa
      481 gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg actgcaatgg tcatccccaa
      541 tatcggtgct tttattgcct ggggatttat aacggcatta tttattccca ccggctggct
      601 gccgaatgaa catttcgcca aaattgttgg tccgatgatt acctatttat tgcccgtgat
      661 gattggttct accggtggtc atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat
      721 tggtacgatt ggtgtgatcg tcggcgctga aatcccgatg ttccttggct cgatgattat
      781 ggggccgctc ggtggtctgg tgattaaata tatcgacaag tcgctggaaa aacgcatacc
      841 tgctggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga tgctcctttg
      901 tctgctgggt tttgaagtta tcggtccggc ggtgttaatt gctaatactt tcgtcaaaga
      961 gtgtattgag gcgctggtac atgcgggtta tctgcctttg ctgtcagtca tcaatgaacc
     1021 ggcgaaagtg cttttcctta ataatgcgat tgatcagggc gtctattatc cgctgggaat
     1081 gcaacaggct tcggttaacg gtaaatccat cttcttcatg gtggcctcca acccaggtcc
     1141 tggtctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta aacgttctgc
     1201 gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt tcccgtatgt
     1261 gctgatgaag ccgctgacca ttattgccat gattgcgggc ggtatgtctg gcacctggat
     1321 gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta tctttgctta
     1381 cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta cggtaggtac
     1441 cctggtgtcc tttgctatca cttcgctgat actgaagatg gaaaaaacgg tggaaacgaa
     1501 gagcgaagat gagtttaccg agtcagccaa tgcggttaag gcgatgaaac aagaaggtgc
     1561 attctcgtta agccgtgtta agcgtattgc ctttgtttgc gatgcaggga tgggctccag
     1621 tgcgatgggc gcaaccacct tccgtaaacg cctggaaaaa gcggggctgg caatagaagt
     1681 aaaacattac gccatagaaa acgtgcctgc ggatgcggat atcgtcgtta ctcacgccag
     1741 tctggaaggg cgcgtgaaac gtgtgacgga taaaccactg atattgatta ataactatat
     1801 tggcgatcca aaactcgaca ctttatttaa tcaattaacc gctgaacata aacactgatt
     1861 acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga tgtccgtctg
     1921 cgcgaatttg aactgccaga aattactgat aatgaactgt tagtgagtgt aatttctgac
     1981 agcgtttgtt tatccacctg gaaagcggcg ttgcttggta gtgaacataa acgcgtcccc
     2041 gacgatttag aaaatcatcc ggttattacc gggcatgaat gtgctggggt tgttgtcgaa
     2101 gtgggtaaaa atctcactgg caaatataaa aaagggcagc gttttgtatt gcaaccggcg
     2161 atggggctac caagcggata ttcagccgga tacagctacg aatattttgg tggcaatgcc
     2221 acctatatga ttattcccga aatcgcgatt aatttaggct gtgtattacc gtatcacggc
     2281 tcttattttg ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat
     2341 gccaattatc acaccacgca atatgtttat gagcatcgta tgggcgtcaa acctggcggc
     2401 aatattgccc tgctggcgtg tgctggcccg atgggcattg gcgctatcga ttacgccatt
     2461 aacggcggca tacaaccggc gcgggtggtg gtagtcgata tcgacgacaa acggctggcg
     2521 caggtgcaga agttgctgcc ggtggagctg gcggccagta aaggcattga gctggtgtat
     2581 atgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac ggatgatgcc
     2641 ggatttgatg acgtatttgt ctatgcagcg gtgccttcag tcgtcgagat ggctgatgaa
     2701 ttactggctg aagatggctg cctgaacttc tttgccggtc cgacggataa aaacttcaaa
     2761 gtgccgttta atttctacaa cgtccattac aacagtacgc acattgtcgg cacgtctggc
     2821 ggttccacgg acgacatgaa agaggcgatt gcccttagcg ccacggggca gttacagccg
     2881 tcgtttatgg tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg
     2941 ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctgaccgcc
     3001 attgccgatt ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagct ggcgcggctg
     3061 gttgagaaaa cgcacggtat ctggaatgaa caggccgaga aatatctgct ggcacaattt
     3121 ggcgttgata ttggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta
     3181 cggaacaggc agcgttggct gcctggccgc aaaccggctg tggcgacaaa aataaaattg
     3241 atggccttgc ggtcaccgcg atgcgccagg cattaaacga cgttgctttt cgtgggcgag
     3301 tggttatcgg cgagggggaa attgaccatg cgccgatgct gtggattggc gaagaggtgg
     3361 gcaaaggcga tgggccagaa gtcgatatcg cggtcgatcc cattgaaggc acgcggatgg
     3421 tggcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcga gatagcctgt
     3481 tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga
     3541 tagatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac
     3601 cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc gcaattgagg
     3661 aagcaacaca gctcggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg
     3721 tgctgacttg ctggcaggat aatccttacg atgtgatgta caccatcggt ggcgcaccgg
     3781 aaggtgttat ttctgcctgt gccgttaaag cattaggcgg ggatatgcag gccgagctga
     3841 ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc
     3901 gcaagcgttg caaggcaatg ggcgttgacg ttaaccgtgt atactcgctc gatgaactgg
     3961 tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg
     4021 gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc
     4081 aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga
     4141 cgctgacaga agatgatgtg cttgagcaac tgaatgcaca ggacaattta ttttcattta
     4201 tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg
     4261 tcgataacga tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc
     4321 cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat
     4381 ggctgtacgc cgatatcacg catttttccc agttttggca ttacctgaac gaacaggatg
     4441 aaacgcccag atttgctgat gacataacct gggattttat cagcaacgtc aatagcataa
     4501 cccgcaatgc gacgctctat gacgcgttga aagcgatgaa gttcgccgat ttcgccgtct
     4561 ggtcagaggc gcgttttagc gggatggtca aaacggcgct gacgctggca gtaacgacca
     4621 ccttaaagga attaacgccg tgaaaatcga actgacggtg aatggactaa aggtacaggc
     4681 gcagtactct gatgatgaaa ttgaaaatgt acataaacca ttgctgcgta tgctggcggc
     4741 cttacagaca gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac
     4801 gggtaaatct accctaacca ccttctggga atatctcgcg caacaagacc cggaactgcc
     4861 cgctattcag acgctgccga tggatggttt tcaccattac aatagctggc tggatgtgca
     4921 tcaattgcga cccttcaaag gcgcaccaga gacattcaac gttgcgaaac tggcggaaaa
     4981 tctgtgccgg gttgtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga
     5041 tcctgttgaa gatgtgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt
     5101 gttgttggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat
     5161 caaagctccc gcatctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggagggtt
     5221 atcgctggca gacgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt
     5281 gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg
     5341 tctgatggat taacgttcct gattacacca tcttccagtc gcaccacacg agagaaatga
     5401 cgccgggtaa atgcggcgtc atgactgatt gctacgacgc tcgtgcctcg ctggcggcac
     5461 ttttccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg
     5521 tccagtagca atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc
     5581 tgcgcactat gcagatctaa cggatgcgcg ctggcagtgt ctgctaactg acagcattgc
     5641 agggcagcat tggtacgttg agtgatttca tccgccgggc atttttgcag tatcaaacca
     5701 aatgcgattt cgtcagcaac ggtgctgtga aaaagctgat tttccgcttc ctgaaacagt
     5761 acgccaatct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcttg ttgttgcagc
     5821 ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt
     5881 cccgcgccat tgtcgccggt cagcgccagc cattcacctt gtttgagttg cagagaaatg
     5941 tcgcaaagac aatccgtagc cgcgccaggc cagcgataag atatctgatt taacgttaac
     6001 atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtgg ttcaggaaag
     6061 gtgtgtgatg acgttcaaaa agaataatca gcgaaccacg ttcaagcgcc cagtgttgta
     6121 agcgctgcaa cagcatttcg cgggcctgcg gcgtcaggcg gctaaaggct tcatcaagaa
     6181 ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacacgctgt gtctctccac
     6241 cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcagg gccagggcgg
     6301 cgtcaatccg cgccataatc tctttttcag caagacacag attttctggc ccaaaggcaa
     6361 cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat tgctggacga
     6421 gctggatggt ggcggattgt tcattaagcg ggagacgccc aatgggcgta ccgagaagcg
     6481 tccccgtgcc ggttatctct cctggtagaa agtccggata ccagcccgcc atcagctgtg
     6541 ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc ggtttgacat
     6601 agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaatag cgaaactgtt
     6661 ctaacgtaac catatccacg ctccgaattc agccagcatt agcaatatca tggcgtaacg
     6721 tgctacgcat tgtagggtgc tatcggctgg tgcccataac gtagtgcgcc tattgttgat
     6781 tcgaaatgcc cgcatatcca gcgccgcacc acggacagca agatcgttta gcgcattgtg
     6841 tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc cctcatccag
     6901 cggcacgccg cgggcgcact gcgcttcatg aataatcgcc aattgccgtt ttaactgctc
     6961 aacgaccaat aacggcccgg caaacagata agcgacgccc ggcggcaggc gagaagcaaa
     7021 caaagcgcgg ataaaacgtt gcacggggac gtactgcatc cacaactgtg aggttgaaac
     7081 aatcgccaga atccgcaacc agagcgtaat agcatgcgcc caccgttctg gagagtgtgg
     7141 cgtgccgctt agccattctg tcagccagcc accgtgcacc agccacagtc cagcccccag
     7201 cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta gcgcaataag
     7261 gcagaaaaag gtcgccgcgc tatagactgg caatattgtc tgtgtaggca ggataagcgt
     7321 ggtgcaggcc gccagcgccc acagcgttaa ggatgtaaaa ggatgcattt agcgcacggc
     7381 agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tgacgcagta aaagccagac
     7441 tatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga
     7501 ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg ctccgcttcc
     7561 tgtcacgccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt
     7621 aataatcacg ccgctaacaa cgacttttgg cagggtgcga aaccaacccg ctcgcgccag
     7681 ccaaccggcg accaggccga tgaccatcgc gacgggcgca aatgcggcgg cgatcgggtc
     7741 ggtcagcaat ccccagagta aattggtcag taaaccggtc agcatcccga ttatcggacc
     7801 aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aaaataggta atttaaccat
     7861 gctggccagt tgcccgccga tcatattgat ggcgatagag ataacgatca acaccagtgc
     7921 ctgactggaa aagtggcgac gcgccataag aaaacctctc tgaaaatagt tatttaacgg
     7981 gggaaatatg caactcgtcg taaccagaaa gcgcgcaaag ctggcggcta aacgccagtt
     8041 cctcaaggca gccgatcacc agttcaagcg tggtccgcac ggtacagccg ggcatcatat
     8101 gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgt tcaccactct
     8161 gttccagcgt gccgttcaat gcgatgactt cgaacttacc gcttagcagg gcagtgtttt
     8221 cttgcccggc atagcgtaag gcgacgtcag tcagactgcc ggtacaacct gcaatccacg
     8281 cggcgtgaag ttgttgttgc tgcgcgaagg cgcgtaattg ggagagaact tcctgcccgg
     8341 ggagcaggcg cagcgcgtaa aagcgtgcgg aggaagaata tgggtagggc aatgtcatca
     8401 ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat cagtcatatt
     8461 ctgatataag tcagggtttg tgaccgcaca acgaaaagat attgctacgc ttattcgtga
     8521 ttctgcaaag tggaataaat tccacagggg cggggatgaa aacgtgacat aagtcagata
     8581 attgtcttct ttgactggac aaacattcct tttattccac gtttcgctta tcctagctga
     8641 agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt gcgacaggta
     8701 aggtttcccc gaacgatttg ctggattact ctgtctggca aatttgctca gggaaaacct
     8761 tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat
     8821 gtggttcgtg ctttgtatga atccggacgc cgtgcggaaa ttaccgtggt ggcaatcaac
     8881 gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag ccatggccgt
     8941 tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga cgccatccgt
     9001 gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc
     9061 ctcgactgca ccggcgtata tggctcccgc gagcatggtg aagcacacat tgccgccggg
     9121 gctaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac cgttgtttat
     9181 ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaatgc ttcctgtacc
     9241 acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat tgagtctggc
     9301 actgtgacca caattcactc cgccatgcat gatcaacagg ttattgatgc ataccatcct
     9361 gacctgcgtc gcactcgggc agccagtcag tcgatcattc cggtcgatac taaactggcc
     9421 gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaggcgat tgcggtacgt
     9481 gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa
     9541 gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt
     9601 gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca cagtgccatt
     9661 gtcgatggca ctcaaacccg ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc
     9721 gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat ggctactgtt
     9781 gctttcaggt aagacgcaag cagcgtctac aaaactttta gaatcaacga gaggattcac
     9841 catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc
     9901 ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc
     9961 tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct
    10021 gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg ttgttaacta
    10081 cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg acggcgttga
    10141 cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa
    10201 agacgacgaa actctgtcca aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc
    10261 attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat tcgctgacgt
    10321 tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag cactgaaaga
    10381 acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca aactgaccgt
    10441 tctggactcc ctgtctaaaa tcgctgacca gctgatcgtt ggtggtggta ttgctaacac
    10501 ctttattgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg acctggttga
    10561 cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc
    10621 aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg tgaaagctga
    10681 cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa
    10741 tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga acttccgcaa
    10801 aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca tcgctggcgg
    10861 cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct cctacatctc
    10921 cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg cagtagcggt
    10981 gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc ccttgtttca
    11041 gcgcgctttt agagcacgca ctttttcata ctctaaataa ttcgagttgc aggaaggcga
    11101 caagagagtg aatcccagga gcttacataa gtaagtgatt ggggtgagcg aacgcagacg
    11161 cagcacatgc aacttgaagt atgacgagta taaggcccga cgatacagga caagagacat
    11221 gtctaagatt tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt
    11281 tttccaggta gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga
    11341 ctccatcaac gccgtgctgg aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt
    11401 ctccaacggt ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg
    11461 tgctgctatc ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta
    11521 tggtgttccg gttatcctgc acaccgacca ctgcgcgaag aaactgctgc cgtggatcga
    11581 cggtctgctg gacgcgggtg aaaaacactt cgcagctacc ggcaagccgc tgttctcttc
    11641 tcacatgatc gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata
    11701 cctggagcgc atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg
    11761 tgaagaagac ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga
    11821 agacgttgat tatgcgtata ccgagctgag caaaatcagc ccgcgtttca ccatcgcagc
    11881 gtccttcggt aacgtacacg gtgtttacaa gccgggtaac gttgttctga ctccgaccat
    11941 cctgcgcgac tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgaa
    12001 cttcgtattc cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta
    12061 cggcgtagtg aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct
    12121 gaactactac aaagcgaacg aagcttacct gcagggtcag ctgggtaacc cgaaaggcga
    12181 agatcagccg aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc
    12241 gatgatcgct cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaagt
    12301 cattcttttc tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg
    12361 tgatgcagat gagaaatctg tgatctattt ggcaaaatta tgctttattg tttacccttg
    12421 tcagactgcc cgccataagg cggcggagtg tatttctcca ttttgagtca gttgaaaagg
    12481 aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga agctggctgg
    12541 tggctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca ctcgcgatca
    12601 tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct
    12661 cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt tacggaatta
    12721 tcgcctttac gctaatcgct gcactggggc gcgtgggcgt acaaacagcg tcagtcattg
    12781 ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca ctgtctaacc
    12841 tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggtgaa tatgttgacc
    12901 tcggcggcgt agccggcact gtgctgagtg tgcagatttt ctccaccacc atgcgtactg
    12961 cagacggtaa aattatcgtt attccgaatg gtaaaattat tgccgggaat attattaact
    13021 tctcccgcga gccagctcgc cgtaacgaat ttattattgg cgtggcgtat gattccgata
    13081 tcgatcaggt taagcagatc ctgaccgata ttatccagtc tgaagatcgc attttgaaag
    13141 atcgcgaaat gactgtgcgt ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc
    13201 gagtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta
    13261 aacgtgaatt tgatgccgcc ggtatcagct tcccgtatcc gcaaatggat gtgaacttta
    13321 agcgggtgaa agaagacaaa gctgcgtaat caacgctgag gccagataat actctctggc
    13381 ctctctctta ttagtttttc tgattgccaa ttaatattat caatttccgc taataacaat
    13441 cccgcgatat agtctctgca tcagatactt aattcggaat atctaacgtg ttttcttatt
    13501 actttcaagg tcttgcactt ggggcggcta tgatcctacc gctcggtcca caaaatgctt
    13561 ttgtgatgaa tcagggcatt cgtcgtcagt accacattat gattgcctta ctttgtgcta
    13621 tcagcgattt ggtcctgatt tgtgcgggga tttttggtgg cagcgcgtta ttgatgcagt
    13681 cgccgtggtt gctggcgctg gtcacctggg gcggcgtagc cttcttgctg tggtatggtt
    13741 ttggcgcttt taaaacagca atgagcagta acattgagtt agccagtgcc gaagtcctga
    13801 aacaaggcag atggaaaatt atcgccacca tgttggcagt gacctggctg aatccgcatg
    13861 tttacctgga tacttttgtt gtgctgggca gccttggcgg gcaacttgat gtggaaccaa
    13921 aacgttggtt tgcgctcggg acaattagcg cctctttcct gtggttcttt ggtctggcta
    13981 ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc
    14041 tggtagtggg atgtgttatg tggtttattg ccttgcagct ggcgagagac ggtattgctc
    14101 atgcacaagc cttgttcagt taggcgctat ctgatggaaa aataaaacag aggcgctaag
    14161 cttgcctcca gaggtcctga attttgggca agtcaattaa cacggagaga ctaacgtgaa
    14221 gttcaaagtt atcgccctgg cggcattaat gggtattagc gggatggcag cgcaggctaa
    14281 cgaattgccg gatggaccgc atattgtcac ctccggtacg gcaagcgtgg atgcggtgcc
    14341 agacattgcc actcttgcga ttgaagttaa cgtggccgcg aaggatgccg ctactgccaa
    14401 gaaacaggca gatgagcgcg tcgcacaata catttccttc cttgagctca atcagatcgc
    14461 gaaaaaagat atcagctcag cgaacttacg cactcagcca gattatgatt atcaggatgg
    14521 taaaagtatc ctgaaaggct accgcgcagt cagaacggtg gaagtcacgc tccgtcagtt
    14581 agacaaactg aattccttgc tggacggcgc gctaaaggcg ggtcttaacg aaattcgttc
    14641 tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac aaagcgcgta aggcagcgat
    14701 tgataacgcg attcatcagg cacaggaact ggcgaatggc tttaatcgta aactggggcc
    14761 ggtatatagc gtgcgctacc atgtttccaa ctatcagccc agcccaatgg tgcggatgat
    14821 gaaagctgat gctgcaccgg tgtccgcaca ggaaacttac gagcaggccg ctattcagtt
    14881 tgatgatcag gtcgatgtag tcttccagtt agaacctgtg gatcaacaac ctgctaaaac
    14941 acctgcagca caataatact tgtagcccga cgtattcgtc gggctaatgc ctttacccga
    15001 tattgcctcc tttttctgta attttctgta aaaccatgac gtaatcacct atctttgctc
    15061 gcggtttcat caaggatagg ctgactgaaa cggttaagcg gacaaggatg cgggagaggg
    15121 gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat
    15181 aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg caaaagcatt
    15241 gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc
    15301 ctcaataaag ccggacagga actgtatcaa aaactgttcc cggtttatca acgactttct
    15361 gcgattgata atgaaatcca taactcagga cgtcgttaac gagaaattgt gataggcata
    15421 gataatactt atccgacaat tatttttgat cagttgatta gtctcggaga taagtacgaa
    15481 ggcgtgacca tccagccagt tgagttcagc gaaaatggcg ttatcgataa tctttttgac
    15541 cgtcagttag attttattat ctccccacag catgtatctg cacgagttca ggaactggaa
    15601 aacctgacta tcagtgaact accaccgctg cgattgggct ttctcgtttc tcgccgttat
    15661 gaagggcgac aggagcaaga gctgcttcag gagctgccct agctacaaat gcgctttcaa
    15721 aaccgggcta acttcgaagc aatgattgat gcgaatatgc gtccttgtgg gatcaatcca
    15781 accattattt atcgtcccta tagttttatg gctaaaatca gcgccgtgga gcggggacat
    15841 tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat
    15901 tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat
    15961 cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    16021 cccagcatcg atccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    16081 tggtgaatat gtccgccgga cgcattttcc agatatcgat gcagataatc ccgatacatg
    16141 ggatgtgcac aatgatcaat ggtggtgcgg ataacgttgt tatacggatc ctgtgcgcca
    16201 aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc gacgttcata
    16261 aacagatcca tgccaatgcc gaagaaaaac gacaggcgag gggcgaagtc atcaattttc
    16321 agcccggcgg agattgctgc tttgatgtac tcaatcctat cggcgagcgt aaatgctacc
    16381 tgctgcacgc agttggcacc cgcttcaccc atgtggtaac cgctgatact gatggtatta
    16441 aatcgcggca tgttgccgga acaccaggcg atgatgtctg cgataatacg cattgacggt
    16501 tttggtgggt aaatataggt gttgcggcaa agatactctt tgaggatatc gttttgaatg
    16561 gtgccggtca gtttatcgga tgtaacacct tgctcttctg cggcgacgat ataaaacgcc
    16621 agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc cagcgggatc
    16681 tggtcgaaca ggacttttat atcttccacg gtgtcgatag cgacgcccgc tttgccgacg
    16741 tcgcccgcca cgcgtgggtt atcggagtcg tagcctcggt gggtggccag gttacggcga
    16801 taaaaagcgt tggactcttt tgctgtcgag aaaccagcat actgacggat ggtccacggt
    16861 tgggcggtat acatagtggc acgcgggcca cgaacgtagg ggggcaaacc aggaagggta
    16921 cccgtcacct ccagattatc gagatcggct tcggtataca gcggcttgat ggcgatccct
    16981 tccgcggttt gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg
    17041 gcaagctgtt gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat
    17101 gagggtattt gcgcttacgg tgagcttttt acgtgggttt gtcgccagca aaaaaatgca
    17161 ttcagacgtc gttttttgcc gcctgagatt tgtctatttt gcgatgtgat tcaaggaata
    17221 caaaaagaag tgaggataac gcctgatatg tgctatcagg cgttatttga tggattaatc
    17281 ctgacgaaga actttgtgac cataatcgag caacgcatca gtgactttac gcatcatgcg
    17341 gctttcagga gcaaagcggt gccagtagag catccggcgt tgaaatagcc ctggcgttaa
    17401 gtcaatcagt tcgccgctgg ccagctcttt ctcgatttgc aggtgcggga tcatacagca
    17461 ggtggtgccc tgacgagcaa gttgcacgaa cgcttccgaa gaattaacga tatggcaggg
    17521 tacgctgcca ggaggcagat cgaaattttg ctgcaaaaag gcctggtgca tatcgtcaag
    17581 atggtcaaac gcgaccactg gcgctttcag taatgccgaa cgcgttacgc cgttagggaa
    17641 atatttttcg gcaaatggtt ttgagctgac gaacagatag tcgagcgcac cgagtttatc
    17701 gacaagacaa ctcggcagcg cctgatgttg aatactcact gcgccgacca cttcgccgcg
    17761 acgcagacgt tcctgagtgc gggtttcatc ttctacctgc aagttgaggc ggatgggcga
    17821 atcagccaac acaggagcca gtgccggaag caaccacgtc gccagactgt cggcgttgac
    17881 cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc actcttcttc
    17941 cagcagctcc acctggcgta gtagcgccaa cagtttttgc ccttgttccg tcgggcgcgg
    18001 cggtacggta cgcactaaca acggctgccc gaacatattt tccagttgct taatgcgctg
    18061 tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg
    18121 tatcaccgca tccagtgcct gtaatgttct gtagtccggg cgtttcattg ctgttgtctg
    18181 ctcctggtaa atgctttttc ctgcactatg acacaatttt gtgtcgggtt gcatataccg
    18241 aagccgttgg cgggtaacat ttgtatcagt ggttaacgcg cagcttaaaa gatcagagat
    18301 ttgcgctaat gcatttcgct cagcgagttc gcgccctggt tgtgttgaat ggcgtggcgt
    18361 tattgcctca gtttgcctgt aaacaggggc ttgcgaacgg ggaactggtg cgcctgttcg
    18421 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaagggatgc
    18481 ccgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
    18541 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    18601 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
    18661 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcact cggtacaatg
    18721 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    18781 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    18841 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    18901 gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta ttgcagacgc ttccaagcag
    18961 gttgatattc tgggcaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    19021 gtggcgcgcc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtagtgacc
    19081 gataacggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    19141 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gtttgtttgc taaccgtggc
    19201 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ctattgtgaa atgatctgac
    19261 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt
    19321 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
    19381 aaatactttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    19441 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
    19501 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    19561 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    19621 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    19681 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
    19741 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    19801 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga actggtgatt
    19861 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcatcgtggc
    19921 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    19981 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    20041 tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt acagcatctt
    20101 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtcaacc
    20161 aaaaatatga tgggcgcgaa agagatttcg ctaatgaaac ccggctcgct gctgattaat
    20221 gcttcgcgcg gtactgtggt ggatattcca gcgctgtgtg acgcgctggc gagcaaacat
    20281 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    20341 acctctccgc tgtgtgaatt cgacaatgtc cttctgacgc cacacattgg cggttcgact
    20401 caggaagcgc aggagaatat tggcctggaa gttgcgggta aattgatcaa gtattctgac
    20461 aatggttcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcacggtggt
    20521 cgtcgtctga tgcacatcca cgaaaaccgt ccgggtgtgc tgactgcgct caacaaaatt
    20581 tttgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc ccagatgggt
    20641 tatgtagtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    20701 aaagctattc cgggtaccat tcgcgcccgt ctgctatact aaatcccttt ctctgaaaat
    20761 caacgggcag gtcactgact tgcccgttat tttatccctt ctccacaccg ttgccgcgtt
    20821 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    20881 gtttatttaa tggggattta cgatggcggg actgtacagg gctgtaacgg taaagccctc
    20941 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    21001 ctgttactca taagtttcag cgcttattaa cagtcagtca caggggagga gcaatccccc
    21061 cttaccctta ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg
    21121 tgtgtcgccc tcaccactcc cagactttcg acggtgtaac caccgcaggc agtgggatat
    21181 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    21241 gcattttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    21301 ggcgctgacc gtactcatca aaggcgacca gcggtgtaat caacacgtct aatcgggaaa
    21361 ggggtagcac gtcacgcaca tccagttttg gctcatggat cttcaacttg ttcatcacca
    21421 gttcgctttg tggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    21481 ctggaagata tacgcgctta ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga
    21541 gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc gggggataag
    21601 tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac
    21661 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    21721 gtaattgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    21781 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    21841 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    21901 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    21961 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    22021 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    22081 cgcgtagtca cgagtcttcg ctttttcttg cgctaactca tagctgatat tcagtgcggc
    22141 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    22201 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    22261 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    22321 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    22381 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    22441 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    22501 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgctgaga
    22561 tgcatggttt aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctgccgc
    22621 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc
    22681 gcaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    22741 atctacctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcgctg gctggttggg
    22801 tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggacaaa gtgaccggcg
    22861 aaaccggtga agccatcgac gatctgcgta acatcgcgca gttgggttac gacgaagacg
    22921 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgagtacgtc cgtgttgccg
    22981 cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac
    23041 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgatat atcccggcaa
    23101 gaatttcagc gtcgccgtca ggccctggta gagcaaatgc aacccggcag cgccgcgctg
    23161 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    23221 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa
    23281 agcgatgaca ctcataacca cagcgttttg tttaaccgcg ttcgcgacct gacggcagag
    23341 atctggtttg gtcgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc
    23401 gcactggcat tcagcgaaat caatcaacaa ctttatcaac tacttaatgg tctggatgtg
    23461 gtttaccacg cccagggcga atatgcatat gctgatgaaa tcgtgaacag cgcgcttgag
    23521 aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt
    23581 cctgttgttc atgaaatgcg cctgttcaaa tcaccagaag agattgccgt acttcgccgc
    23641 gcgggtgaaa tcaccgcact ggcgcataca cgggcgatgg aaaaatgccg tccggggatg
    23701 ttcgagtacc atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgttac
    23761 ccttcctata acaccattgt tggcagcggt gaaaacggct gcattttgca ttacaccgaa
    23821 aacgagtgtg aactgcgtga cggtgacctg gtgttgattg acgcgggttg tgaatacaaa
    23881 ggttacgctg gcgacattac ccgcaccttc ccggtcaacg gcaaattcac tcaggctcag
    23941 cgtgaaatct atgacattgt gctggagtct cttgaaacca gcctgcgcct gtatcgtccg
    24001 ggaacttcca ttcaggaagt cactggtgaa gtggtgcgca tcatggttag cggtctggta
    24061 aaactcggca tcctgaaagg tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct
    24121 ttctttatgc atggccttag ccactggtta ggactggatg ttcatgacgt tggtgtttat
    24181 ggtcaggatc gctcgcgtat tctggaaccg ggtatggtac tgaccgtaga accaggactg
    24241 tatattgcgc cggatgcaga agtgccagaa cagtatcgcg gtatcggcat tcgtattgaa
    24301 gacgatattg tgattaccga aaccggtaat gaaaacctca ccgccagcgt ggtgaaaaag
    24361 ccggaagaaa tcgaagcgtt gatggcagct gcgagaaagc aatgagcgta atcatcgtcg
    24421 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    24481 tgccagtaca tttgattgaa gcgaccgcgc cagagtcaca tgcgcatccg ggctttgatg
    24541 gacgagctat tgcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc
    24601 aatctctggc tgattgcgca actgccatca ccaccgtgca tgttagcgat cgtggtcacg
    24661 ctggatttgt caccctcgcc gcagaagatt accaactggc ggcgctgggg caggttgtcg
    24721 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg caaagcacct ggcgtaacgc
    24781 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagccacgtt gaagtgacgc
    24841 tggagagtgg cgagacgctg acgggccgcg tgctggtagc cgctgatggc acccattcag
    24901 cgttagccac cgcgtgcggt gttgactggc agcaggagcc ttacgaacaa ctggctgtga
    24961 ttgccaacgt tgcgacttcc gttgcgcatg aagggcgcgc ttttgaacgc tttacacaat
    25021 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    25081 cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga gaagttctgc cgtgaactcc
    25141 agtcggcctt tggctggcga ctggggcaaa ttacccacgc cggaaaacgt agtgcttatc
    25201 cgctggcgtt aacccgcgcc gccaaaccta ttacccatcg taccgtgctg gtggggaatg
    25261 cggcgcaaac tctgcatccc atcgccgggc aaggatttaa cctcggtatg cgtgatgtga
    25321 tgagtcttgc agaaaccctg actcaggcgc aggaacgcag agaagacatg ggtgattacg
    25381 gcgtattgtg ccgttatcag cagcgtcgac agcgcgatcg cgaagcaacc atcggcgtca
    25441 cggacagcct tgtacatctt tttgccaacc gttggacgcc gctggttgtc gggcgcaaca
    25501 tcgggctgat gacgatggaa ttatttaccc cggcacgcga tgtgctggcg cagcgcactc
    25561 ttggttgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta
    25621 gccattgttg gcggcggcat ggtcgggctg gcggttgcct gcggcttaca gggtatcggc
    25681 ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc tggcggcgga tgcaccacca
    25741 caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc
    25801 tggcaggaca ttctctctcg tagggcctgt tgctatcacg gcatggaagt gtgggacaaa
    25861 gacagctttg gtcacatctc gtttgacgat caaagcatgg gctatagcca tcttgggcat
    25921 atcgttgaaa actcagtgat tcactacgcg ctgtggaaca aagcgctgca gtcgtcagat
    25981 atcactctgt tggcccccgc agaattacag caggtcgcct ggggtgaaaa tgaaaccttc
    26041 ctgacgttga aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggcgct
    26101 aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta tcagcatcac
    26161 gcgctggtgg cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg acaggttttt
    26221 catggcgaag gcattctggc ctttttgccg cttagcgatc cgcatctttg ctcgattgtc
    26281 tggtcactgt cgccagagga agcgcagcgg atgcagcagg caggcgaaga cgaatttaat
    26341 cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga aagtgagcgc
    26401 ctggtgttcc cgctaacggg gcgttatgcg cgccagtttg ccgcgcaccg tctggcgctg
    26461 gtgggcgacg ccgcgcatac cattcacccg ttggcggggc agggggtaaa tctcggcttt
    26521 atggatgctg cagagctgat tgctgaactg aaacggttgc atcgtcaggg taaagacatc
    26581 gggcagtaca tttacctgcg tcgctatgag cgtagccgca agcacagcgc ggcgctgatg
    26641 ctggctggta tgcagggatt ccgcgatctg ttttccgggg ctaatccggc gaaaaaattg
    26701 ctgcgtgata ttggtctgaa actggctgat acgcttcctg gcgttaagcc acaacttatt
    26761 cgccaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcttctgc
    26821 tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc
    26881 atttgaaata aactaatttc acctgcgatt tcgcattata ttttctaatg ccacgaattt
    26941 ttgatttagt gttt
//
LOCUS       DAJOTB010000056        26889 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJOTB010000056 DAJOTB010000000
VERSION     DAJOTB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.056"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            54..1454
                     /gene="gspA"
                     /locus_tag="OHN28_002296"
     CDS             54..1454
                     /gene="gspA"
                     /locus_tag="OHN28_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCQ2297690.1"
                     /translation="MLGEAGSGKTRLAQQLQKDKHRRVITLSLVVSWQGKAAWIVTDD
                     NAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIENAHLNHRRILDDLQRAISLI
                     PDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHELKASILEGQNIDQPDLLLTARV
                     LKRIALLCRGDRRKLALAGETISLLQQAEQTCVFTAKQWRMIYRVLGDNRPRKMQLAV
                     VMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPVVKQDMTKDIAHVVMRDSEALSVL
                     YGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTLVDQNLPWIASLKVGDKKLPVVVV
                     RVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLWKMSPEGESTITRDSSEEEILWLE
                     TMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYL
                     YRDEANISPETTVKGK"
     gene            1456..1875
                     /gene="gspB"
                     /locus_tag="OHN28_002297"
     CDS             1456..1875
                     /gene="gspB"
                     /locus_tag="OHN28_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCQ2297691.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2113..2424
                     /gene="rpsJ"
                     /locus_tag="OHN28_002298"
     CDS             2113..2424
                     /gene="rpsJ"
                     /locus_tag="OHN28_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCQ2297692.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2457..3086
                     /gene="rplC"
                     /locus_tag="OHN28_002299"
     CDS             2457..3086
                     /gene="rplC"
                     /locus_tag="OHN28_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCQ2297693.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3097..3702
                     /gene="rplD"
                     /locus_tag="OHN28_002300"
     CDS             3097..3702
                     /gene="rplD"
                     /locus_tag="OHN28_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCQ2297694.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3699..4001
                     /gene="rplW"
                     /locus_tag="OHN28_002301"
     CDS             3699..4001
                     /gene="rplW"
                     /locus_tag="OHN28_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCQ2297695.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4019..4840
                     /gene="rplB"
                     /locus_tag="OHN28_002302"
     CDS             4019..4840
                     /gene="rplB"
                     /locus_tag="OHN28_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCQ2297696.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4857..5135
                     /gene="rpsS"
                     /locus_tag="OHN28_002303"
     CDS             4857..5135
                     /gene="rpsS"
                     /locus_tag="OHN28_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCQ2297697.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5150..5482
                     /gene="rplV"
                     /locus_tag="OHN28_002304"
     CDS             5150..5482
                     /gene="rplV"
                     /locus_tag="OHN28_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCQ2297698.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5500..6201
                     /gene="rpsC"
                     /locus_tag="OHN28_002305"
     CDS             5500..6201
                     /gene="rpsC"
                     /locus_tag="OHN28_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCQ2297699.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6214..6624
                     /gene="rplP"
                     /locus_tag="OHN28_002306"
     CDS             6214..6624
                     /gene="rplP"
                     /locus_tag="OHN28_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCQ2297700.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6624..6815
                     /gene="rpmC"
                     /locus_tag="OHN28_002307"
     CDS             6624..6815
                     /gene="rpmC"
                     /locus_tag="OHN28_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCQ2297701.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6815..7069
                     /gene="rpsQ"
                     /locus_tag="OHN28_002308"
     CDS             6815..7069
                     /gene="rpsQ"
                     /locus_tag="OHN28_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCQ2297702.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7234..7605
                     /gene="rplN"
                     /locus_tag="OHN28_002309"
     CDS             7234..7605
                     /gene="rplN"
                     /locus_tag="OHN28_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCQ2297703.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7616..7930
                     /gene="rplX"
                     /locus_tag="OHN28_002310"
     CDS             7616..7930
                     /gene="rplX"
                     /locus_tag="OHN28_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCQ2297704.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7945..8484
                     /gene="rplE"
                     /locus_tag="OHN28_002311"
     CDS             7945..8484
                     /gene="rplE"
                     /locus_tag="OHN28_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCQ2297705.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8499..8804
                     /gene="rpsN"
                     /locus_tag="OHN28_002312"
     CDS             8499..8804
                     /gene="rpsN"
                     /locus_tag="OHN28_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCQ2297706.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8838..9230
                     /gene="rpsH"
                     /locus_tag="OHN28_002313"
     CDS             8838..9230
                     /gene="rpsH"
                     /locus_tag="OHN28_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCQ2297707.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9243..9776
                     /gene="rplF"
                     /locus_tag="OHN28_002314"
     CDS             9243..9776
                     /gene="rplF"
                     /locus_tag="OHN28_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCQ2297708.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9786..10139
                     /gene="rplR"
                     /locus_tag="OHN28_002315"
     CDS             9786..10139
                     /gene="rplR"
                     /locus_tag="OHN28_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCQ2297709.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10154..10657
                     /gene="rpsE"
                     /locus_tag="OHN28_002316"
     CDS             10154..10657
                     /gene="rpsE"
                     /locus_tag="OHN28_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCQ2297710.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10661..10840
                     /gene="rpmD"
                     /locus_tag="OHN28_002317"
     CDS             10661..10840
                     /gene="rpmD"
                     /locus_tag="OHN28_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCQ2297711.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10844..11278
                     /gene="rplO"
                     /locus_tag="OHN28_002318"
     CDS             10844..11278
                     /gene="rplO"
                     /locus_tag="OHN28_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCQ2297712.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11286..12617
                     /gene="secY"
                     /locus_tag="OHN28_002319"
     CDS             11286..12617
                     /gene="secY"
                     /locus_tag="OHN28_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCQ2297713.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12649..12765
                     /gene="rpmJ"
                     /locus_tag="OHN28_002320"
     CDS             12649..12765
                     /gene="rpmJ"
                     /locus_tag="OHN28_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCQ2297714.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12912..13268
                     /gene="rpsM"
                     /locus_tag="OHN28_002321"
     CDS             12912..13268
                     /gene="rpsM"
                     /locus_tag="OHN28_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCQ2297715.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13285..13674
                     /gene="rpsK"
                     /locus_tag="OHN28_002322"
     CDS             13285..13674
                     /gene="rpsK"
                     /locus_tag="OHN28_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCQ2297716.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13708..14328
                     /gene="rpsD"
                     /locus_tag="OHN28_002323"
     CDS             13708..14328
                     /gene="rpsD"
                     /locus_tag="OHN28_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCQ2297717.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14354..15343
                     /gene="rpoA"
                     /locus_tag="OHN28_002324"
     CDS             14354..15343
                     /gene="rpoA"
                     /locus_tag="OHN28_002324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCQ2297718.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15384..15767
                     /gene="rplQ"
                     /locus_tag="OHN28_002325"
     CDS             15384..15767
                     /gene="rplQ"
                     /locus_tag="OHN28_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCQ2297719.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15874..16242
                     /locus_tag="OHN28_002326"
     CDS             15874..16242
                     /locus_tag="OHN28_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCQ2297720.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16253..16678
                     /gene="zntR"
                     /locus_tag="OHN28_002327"
     CDS             16253..16678
                     /gene="zntR"
                     /locus_tag="OHN28_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCQ2297721.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16734..16952
                     /gene="arfA"
                     /locus_tag="OHN28_002328"
     CDS             16734..16952
                     /gene="arfA"
                     /locus_tag="OHN28_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCQ2297722.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16949..17362)
                     /gene="mscL"
                     /locus_tag="OHN28_002329"
     CDS             complement(16949..17362)
                     /gene="mscL"
                     /locus_tag="OHN28_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCQ2297723.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17492..18868)
                     /gene="trkA"
                     /locus_tag="OHN28_002330"
     CDS             complement(17492..18868)
                     /gene="trkA"
                     /locus_tag="OHN28_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCQ2297724.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18890..20179)
                     /gene="rsmB"
                     /locus_tag="OHN28_002331"
     CDS             complement(18890..20179)
                     /gene="rsmB"
                     /locus_tag="OHN28_002331"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCQ2297725.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20231..21178)
                     /gene="fmt"
                     /locus_tag="OHN28_002332"
     CDS             complement(20231..21178)
                     /gene="fmt"
                     /locus_tag="OHN28_002332"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCQ2297726.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21193..21702)
                     /gene="def"
                     /locus_tag="OHN28_002333"
     CDS             complement(21193..21702)
                     /gene="def"
                     /locus_tag="OHN28_002333"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCQ2297727.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21832..22956
                     /gene="dprA"
                     /locus_tag="OHN28_002334"
     CDS             21832..22956
                     /gene="dprA"
                     /locus_tag="OHN28_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCQ2297728.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIIGGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22928..23401
                     /locus_tag="OHN28_002335"
     CDS             22928..23401
                     /locus_tag="OHN28_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCQ2297729.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23430..23972
                     /locus_tag="OHN28_002336"
     CDS             23430..23972
                     /locus_tag="OHN28_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCQ2297730.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            23977..24549
                     /gene="tsaC"
                     /locus_tag="OHN28_002337"
     CDS             23977..24549
                     /gene="tsaC"
                     /locus_tag="OHN28_002337"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCQ2297731.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24554..25372
                     /gene="aroE"
                     /locus_tag="OHN28_002338"
     CDS             24554..25372
                     /gene="aroE"
                     /locus_tag="OHN28_002338"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCQ2297732.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25369..25626
                     /locus_tag="OHN28_002339"
     CDS             25369..25626
                     /locus_tag="OHN28_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCQ2297733.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25602..26156)
                     /locus_tag="OHN28_002340"
     CDS             complement(25602..26156)
                     /locus_tag="OHN28_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCQ2297734.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
     gene            26627..>26889
                     /locus_tag="OHN28_002341"
     rRNA            26627..>26889
                     /locus_tag="OHN28_002341"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6877 a         6324 c         7024 g         6664 t
ORIGIN      
        1 aagtcattct ttcctggttg cgtgagaagc atcagacctg gcgtctgtgc tatttgttgg
       61 gtgaggctgg aagtggaaaa acccggctgg cgcagcaact acaaaaagat aaacatcgcc
      121 gtgtgattac tttaagcctc gttgtttcct ggcaaggtaa ggccgcatgg atcgttaccg
      181 acgataatgc ggctgaacag ggctgccgtg acagcgcctg gacgcgagat gagatggcgg
      241 gtcaattact gcatgcactt catcgaactg atagtcgctg ccctcttatt attattgaaa
      301 atgctcacct gaatcaccgc cgcatacttg atgatctcca acgcgcgata tctcttattc
      361 ctgacggtca atttttgctg atcggcagac ccgatcgaaa agtcgaacgt gattttaaaa
      421 aacagggcat tgaacttgtc tcgacaggtc gtctgacgga gcacgagctt aaggcaagca
      481 tccttgaagg tcagaatatt gaccagcccg atctcctttt aaccgccaga gttctgaaac
      541 ggatagcttt attatgtcgg ggagatcgca gaaagctggc gcttgccggc gaaacaatta
      601 gcttattaca gcaagccgag cagacctgcg ttttcactgc aaaacaatgg cgaatgattt
      661 accgcgtatt aggtgataac cgcccccgga aaatgcagct tgccgtggtg atgagtggaa
      721 ccattatcgc acttacctgt ggttggctac tgctgccctc tttcacagct ccgttacccg
      781 ttcctgcgtg gctgataccc gtaacgccag tagttaaaca agatatgact aaggatattg
      841 cccatgtggt gatgcgcgat agtgaggcgc taagcgtgtt atacggcgta tgggggtacg
      901 aggtcccagc cgattcggcc tggtgcgatc aggcagtaag agcggggctg gtatgtaaaa
      961 gtgggaatgc ttctttacaa acattggtag atcagaattt gccctggatc gcatcgctta
     1021 aagtcgggga caaaaaattg cctgttgtgg tcgttcgcgt cggtgatgct accgttgatg
     1081 tactcgtagg gcagcaaacc tggacgctga cacataaatg gtttgagttg gtatggacgg
     1141 gtgattatct tctgttgtgg aaaatgtcgc cggaaggaga gagtaccatt acgcgtgata
     1201 gcagcgagga agagattctc tggctggaaa cgatgttaaa ccgcgcattg catatttcga
     1261 cagaatcttc ggcagagtgg cgtcccttgt tggtagaaaa aataaagcaa tttcaaaaaa
     1321 gccatcactt gaaaactgac ggtgttgttg gcttttcaac gttagtgcat ctctggcagg
     1381 tggcagggga gagtgcctac ttatatcggg atgaagcaaa tatttccccg gaaacaacag
     1441 tgaaggggaa atgacatgtt tgaattctat atcgccgcgc gtgagcagaa agaaacaggt
     1501 caccctggta tcttctcccg acaaaaacac agcaccataa tatatgtcat ttgtttattg
     1561 ctgatttgcc tgtggtttgc aggaatggtg ttggttggtg ggtatgcccg gcagctatgg
     1621 gcactttgga tagtaaaagc agaagtcact gtagatgctg aaacgcccgc ttttaaacaa
     1681 tcaacacaac actatttttt caaaaaacag ccactccccg tcgtcgaatc cgtcgaggag
     1741 gaagatgacc caggagtcgc ggttgagaat gccccctctt cttctgaaga cgaagacaat
     1801 actgtagaag aaagcgacga gaaagccggt ttaagagagc gtgtcaaaaa tgcgctgaac
     1861 gaacttgaga gataacccaa aggctgttta ctttactagc aatacgcttg cgttcggtgg
     1921 ttaagtatgt ataatgcgcg ggcttgtcgt agttgacagc aggttcaatc tgaacctcag
     1981 ttagccgaat ttggctacct aacaatgctc ccaatcgggg agctacgtaa gaacggttac
     2041 actctcccat caatcgtaat gggtctgagg agtaatcatt ttcgtttata aaataattgg
     2101 agctctggtc tcatgcagaa ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt
     2161 ctgatcgatc aagcaaccgc ggaaatcgtc gagactgcta agcgcactgg tgcgcaggtc
     2221 cgtggtccga tcccgctgcc gacacgcaaa gagcgcttca ctgttctgat ctccccgcac
     2281 gtcaacaaag acgcgcgcga tcagtacgaa atccgtactc acttgcgtct ggttgacatc
     2341 gttgagccaa ccgagaaaac cgttgatgct ctgatgcgtc tggatctggc tgccggtgta
     2401 gacgtgcaga tcagcctggg ttaatcaggt cattgagcga ttgagaggtt gaaacaatga
     2461 ttggtttagt cggtaaaaaa gtgggtatga cccgtatctt cacagaagac ggcgtttcta
     2521 tcccagtaac cgtaatcgaa gttgaagcaa accgcgttac tcaggttaaa gacctggcta
     2581 acgatggcta ccgtgctatt caggtgacca ccggtgctaa aaaagctaac cgtgtgacca
     2641 agcctgaagc tggccacttc gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat
     2701 tccgcctggc tgaaggcgaa gagttcactg taggtcagag cattagcgtt gaactgtttg
     2761 ctgacgttaa aaaagttgac gtaactggca cctctaaagg taaaggtttc gcaggtaccg
     2821 ttaagcgctg gaacttccgt acccaggacg ctactcacgg taactccttg tctcaccgcg
     2881 ttccgggttc tatcggtcag aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg
     2941 caggtcagat gggtaacgaa cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg
     3001 ctgagcgcaa cctgctgctg gttaaaggcg ctgtcccggg tgcaaccggt agcgacctga
     3061 tcgttaaacc agctgtgaag gcgtaaggag atagcaatgg aattagtatt gaaagacgcg
     3121 cagagcgcgc tgactgtttc cgaaactacc ttcggtcgtg atttcaacga agcgctggtt
     3181 caccaggttg ttgttgctta tgcagctggt gctcgtcagg gtactcgtgc tcagaagact
     3241 cgtgctgaag taactggttc cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg
     3301 cgttctggtt ctatcaagag cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt
     3361 ccgcaggacc acagtcaaaa agttaacaag aagatgtacc gcggcgcgct gaaaagcatc
     3421 ctgtccgaac tggtacgtca ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg
     3481 ccgaaaacta agctgctggc acagaaactg aaagacatgg ctctggaaga tgtgctgatc
     3541 atcaccggtg agctggacga gaacctgttc ctggctgcgc gcaacctgca caaggttgac
     3601 gtacgcgatg caactggtat cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg
     3661 actgctgatg ctgttaagca agttgaggag atgctggcat gattcgtgaa gaacgtctgc
     3721 tgaaggtgct gcgtgcaccg cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca
     3781 acaccatcgt actcaaagtt gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc
     3841 agaaactgtt tgaagtcgaa gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta
     3901 aacgtcacgg acagcgtatc ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga
     3961 aagaaggcca gaatctggac ttcgttggcg gcgctgagta agtcggagga gtaatacaat
     4021 ggcagttgtt aaatgtaaac cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa
     4081 ccctgagctg cacaagggca aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg
     4141 tggtcgtaac aacaatggcc gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc
     4201 ttaccgtatt gttgacttca aacgcaacaa agacggtatc ccggcagttg ttgaacgtct
     4261 tgagtacgat ccgaaccgtt ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg
     4321 ccgttacatc ctggccccta aaggcctgaa agctggcgac cagattcagt ctggcgttga
     4381 tgctgcaatc aaaccaggta acaccctgcc gatgcgcaac atcccggttg gttctactgt
     4441 tcataacgta gaaatgaaac caggtaaagg cggtcagctg gcacgttccg ctggtactta
     4501 cgttcagatc gttgctcgtg atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat
     4561 gcgtaaagta gaagcagact gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat
     4621 gctgcgcgtt ctgggtaaag caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg
     4681 cggtaccgcg atgaacccgg tagaccaccc acatggtggt ggtgaaggtc gtaactttgg
     4741 taagcacccg gtaactccgt ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa
     4801 gcgtactgat aaattcatcg tacgtcgccg tagcaaataa ttttagagga taagccatgc
     4861 cacgttctct caagaaaggt ccttttattg acctgcactt gctgaagaag gtagagaaag
     4921 cggtggaaag cggagacaag aagcccctgc gcacttggtc ccgtcgttca acgatctttc
     4981 ctaacatgat cggtttgacc atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg
     5041 taaccgacga aatggtcggt cacaaactgg gtgaattcgc accgactcgt acttatcgcg
     5101 gccacgctgc tgataaaaaa gcgaagaaga aataaggtag gaggaagaga tggaaactat
     5161 cgctaaacat cgccatgctc gttcttctgc tcagaaggtt cgccttgttg ctgacctgat
     5221 tcgcggtaag aaagtgtcgc aggctctgga tattttgacc tacaccaaca agaaagcggc
     5281 tgtactggtc aagaaagttc tggaatctgc cattgctaac gctgaacaca acgatggcgc
     5341 tgacattgac gatctgaaag ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg
     5401 cattatgccg cgtgcaaaag gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac
     5461 tgtggttgtg tccgatcgct gagactctgg agactagcaa tgggtcagaa agtacatcct
     5521 aatggtattc gcctgggtat tgtaaaacca tggaactcta cctggtttgc gaacaccaaa
     5581 gaattcgctg acaacctgga cagcgatttt aaagtacgtc agtacctgac taaggaactg
     5641 gctaaagcgt ccgtatctcg tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc
     5701 attcacactg ctcgcccggg tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg
     5761 cgtaaggtcg tagcggacat cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt
     5821 aagcctgaac tggacgcaaa actggttgct gacagcatca cttctcagct ggaacgtcgc
     5881 gttatgttcc gtcgtgctat gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa
     5941 ggtattaaag ttgaagttag cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg
     6001 taccgcgaag gtcgcgtacc gctgcacact ctgcgtgctg acatcgacta caacacctct
     6061 gaagcgcaca ccacttacgg tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc
     6121 ctgggtggta tggctgctgt tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag
     6181 cagcgtaaag gccgtaaata aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc
     6241 cgtaaaatgc acaaaggccg taaccgcggt ctggcgcagg gtacggatgt tagcttcggc
     6301 agcttcggtc tgaaagctgt tggccgtggt cgtctgactg cacgtcagat cgaagcagca
     6361 cgtcgtgcta tgacccgtgc agttaagcgt caaggtaaga tctggatccg tgtgttcccg
     6421 gacaaaccga tcactgaaaa gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg
     6481 gagtattggg ttgccttgat tcagccgggt aaagtcctgt atgaaatgga cggtgttccg
     6541 gaagagctgg cccgtgaagc attcaagctg gcagcagcga aactgccgat taaaaccacc
     6601 tttgtaacta agacggtgat gtaatgaaag caaaagagct gcgtgagaag agcgttgaag
     6661 agctgaacac cgagctgctg aacctgctgc gtgagcagtt caacctgcgt atgcaggctg
     6721 caagtggcca gctgcaacag tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg
     6781 ttaagacttt actgaacgag aaggcgggtg cgtaatgacc gataaaatcc gtactctgca
     6841 aggtcgcgtt gttagcgaca aaatggagaa atccattgtt gttgctatcg aacgttttgt
     6901 gaaacacccg atctacggta aattcatcaa gcgtacgacc aaactgcacg tacatgacga
     6961 gaacaacgaa tgcggtatcg gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa
     7021 gactaaatcc tggacgctgg ttcgcgttgt agagaaagcg gttctgtaat acagtacact
     7081 ctctcgatac gaataaacgg ctcagaaatg agccgtttat tttttctacc catatccttg
     7141 aagcggtgtt ataatgccgc gccctcgata tggggatttt taacgacctg attttcgggt
     7201 ctcagtagta gttgacatta gcggagcact aaaatgatcc aagaacagac tatgctgaac
     7261 gtcgccgaca actccggtgc acgtcgcgta atgtgtatca aggttctggg tggctcgcac
     7321 cgtcgctacg caggcgtagg cgatatcatc aagatcacca tcaaagaagc aattccgcgt
     7381 ggtaaggtca aaaaaggtga tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt
     7441 cgtcgcccgg acggttctgt cattcgcttc gatggtaatg cttgtgttct tctgaacaac
     7501 aacagcgagc agcctatcgg tacgcgtatt tttgggccgg taactcgtga gcttcgtagt
     7561 gagaagttca tgaaaattat ctctctggca ccagaagtac tctaaggagc gaatcatggc
     7621 agcgaaaatc cgtcgtgatg acgaagttat cgtgttaacc ggtaaagata aaggtaaacg
     7681 cggtaaagtt aagaatgtcc tgtcttccgg caaggtcatt gttgaaggta tcaatctggt
     7741 taagaaacat cagaagccgg ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga
     7801 agccgctatt caggtttcca acgtagcaat cttcaatgcg acaaccggca aggctgaccg
     7861 tgtaggcttt agattcgaag acggtaaaaa agtccgtttc ttcaagtcta acagcgaaac
     7921 tatcaagtaa tttggagtag tacgatggcg aaactgcatg attactacaa agacgaagta
     7981 gttaaaaaac tcatgactga gtttaactac aattctgtca tgcaagtccc tcgggtcgag
     8041 aagatcaccc tgaacatggg tgttggtgaa gcgatcgctg acaaaaaact gctggataac
     8101 gcagcagcag acctggcagc aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa
     8161 tctgttgcag gcttcaaaat ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt
     8221 ggcgaacgca tgtgggagtt ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt
     8281 gacttccgtg gcctgtccgc taagtctttc gacggtcgtg gtaactacag catgggtgtc
     8341 cgtgagcaga tcatcttccc agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg
     8401 gatattacca ttaccactac tgcgaaatct gacgaagaag gccgcgctct gctggctgcc
     8461 tttgacttcc cgttccgcaa gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag
     8521 cacgcgaagt aaaacgcgta gctttagctg ataaatactt cgcgaaacgc gctgaactga
     8581 aagcgatcat ctctgatgtg aacgcttccg acgaagatcg ttggaacgct gttctcaagc
     8641 tgcagactct gccgcgtgat tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag
     8701 gtcgtccgca tggtttcctg cggaagttcg ggttgagccg tattaaggtc cgtgaagccg
     8761 ctatgcgcgg tgaaatcccg ggtctgaaaa aggctagctg gtaattgtca ccaattgaat
     8821 cacgggagta aagacagatg agcatgcaag atccgatcgc ggatatgctg acccgtatcc
     8881 gtaacggtca ggccgcgaac aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg
     8941 caatcgccaa cgtgctgaag gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca
     9001 ccaagcctga actggaactt actctgaagt atttccaggg caaagctgtt gtagaaagca
     9061 ttcagcgtgt cagccgccca ggtctgcgca tctataaacg taaagatgag ctgccgaaag
     9121 ttatggcggg tctgggtatc gcagttgttt ctacctctaa aggtgttatg actgatcgtg
     9181 cagcgcgcca ggctggtctt ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa
     9241 aaatgtctcg tgttgctaaa gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca
     9301 acggtcaggt tattacgatc aaaggtaaaa acggcgagct gactcgtact ctcaacgatg
     9361 ctgttgaagt taaacatgca gataataccc tgaccttcgg tccgcgtgat ggttacgcag
     9421 acggttgggc acaggctggt accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta
     9481 ccgaaggctt cactaagaag ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag
     9541 gcaatgtgat taacctgtct ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg
     9601 gtatcactgc tgaatgtccg actcagactg aaatcgtgct gaaaggcgct gataagcagg
     9661 tgatcggcca ggttgcagcg gatctgcgcg cctaccgtcg tcctgagcct tacaaaggca
     9721 agggtgttcg ttacgccgac gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta
     9781 acactatgga taagaaatct gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc
     9841 aggagctggg cgcaactcgc ctggtggtac atcgtacccc gcgtcacatt tacgcacagg
     9901 taattgcacc gaacggttct gaagttctgg tagctgcttc tactgtagaa aaagctatcg
     9961 ctgaacaact gaagtacacc ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg
    10021 ctgaacgcgc tctggaaaaa ggcatcaaag atgtatcctt tgaccgttcc gggttccaat
    10081 atcatggtcg tgtccaggca ctggcagatg ctgcccgtga agctggcctt cagttctaag
    10141 gtagaggtgt aagatggctc acatcgaaaa acaagctggc gaactgcagg aaaagctgat
    10201 cgcggtaaac cgcgtatcta aaaccgttaa aggtggtcgt attttctcct tcacagctct
    10261 gactgtagtt ggcgatggta acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt
    10321 tccagcagcg atccagaaag cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct
    10381 gaataacggc actctgcaac accctgttaa aggtgttcac acgggttctc gcgtattcat
    10441 gcagccggct tccgagggta ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga
    10501 agtcgctggg gttcataacg ttctggctaa agcgtatggt tccaccaacc cgattaacgt
    10561 ggttcgtgca actatcgatg gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa
    10621 gcgtggtaaa tccgttgaag aaattctggg gaaataaacc atggcaaaga ctattaaaat
    10681 tactcaaacc cgcagtgcaa tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct
    10741 gggtctgcgt cgtattggtc acaccgtaga gcgcgaggat actcctgcta ttcgcggtat
    10801 gatcaacgcg gtttccttca tggttaaagt tgaggagtaa gagatgcgtt taaatactct
    10861 gtctccggcc gaaggctcca aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg
    10921 cctcggtaaa accggtggtc gtggtcacaa aggtcagaaa tctcgttctg gcggtggcgt
    10981 acgtcgcggt ttcgagggtg gtcagatgcc tctgtaccgt cgtctgccga aattcggctt
    11041 cacttctcgt aaagcagcga ttacagccga agttcgtctg tctgacctgg ctaaagtaga
    11101 aggcggtgtt gtagacctga acacgctgaa agcggctaac attatcggta tccagatcga
    11161 gttcgcgaaa gtgatcctgg ctggcgaagt aacgactccg gtaactgttc gtggcctgcg
    11221 tgttactaaa ggcgctcgtg ctgctatcga agctgctggc ggtaaaatcg aggaataagt
    11281 agcagatggc taaacaaccg ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc
    11341 tgaaacgcag actgctgttt gttatcggtg cgctgattgt gttccgtatt ggctctttta
    11401 ttccgatccc tggtattgat gccgctgtac ttgccaaact gcttgagcaa cagcgaggca
    11461 ccatcattga gatgtttaac atgttctctg gtggtgctct cagccgtgct tctatctttg
    11521 ctctggggat catgccgtat atttcggcgt cgatcattat ccagctactg acggtggttc
    11581 atccaacgtt ggcagaaatt aagaaagaag gggagtctgg tcgtcgtaag atcagccagt
    11641 acacccgcta cggtactctg gtgctggcaa tattccagtc gatcggtatt gctactggtc
    11701 tgccgaatat gcctggtatg caaggcctgg tgattaaccc gggctttgca ttctacttca
    11761 ccgctgttgt aagtctggtc acaggaacca tgttcctgat gtggttgggc gaacagatca
    11821 ctgaacgagg tatcggcaac ggtatttcaa tcattatctt cgccggtatt gtcgcgggac
    11881 tcccgccagc cattgcccat actatcgagc aagcgcgtca aggcgacctg cacttcctcg
    11941 tgttgctgtt ggttgcagta ttagtatttg cagtgacgtt ctttgttgta tttgttgagc
    12001 gtggtcaacg ccgcattgtg gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg
    12061 ctgcacagag cacacattta ccgctgaaag tgaatatggc gggggtaatc ccggcaatct
    12121 tcgcttccag tattattctg ttcccggcga ccatcgcgtc atggttcggg ggcggtactg
    12181 gttggaactg gctgacaaca atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt
    12241 tactctatgc gtctgcaatc atcttcttct gtttcttcta cacggcgttg gttttcaacc
    12301 cgcgtgaaac agcagataac ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg
    12361 gagagcaaac ggcgaagtat atcgataaag taatgacccg cctgaccttg gttggtgcgc
    12421 tgtacattac ctttatctgc ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt
    12481 tctacttcgg tgggacctca ctgcttatcg ttgttgtcgt gattatggac tttatggctc
    12541 aagtgcaaac tctgatgatg tccagtcagt atgagtctgc attgaagaag gcgaacctga
    12601 aaggctacgg ccgataattg gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt
    12661 gcttccgtca agaaattatg ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt
    12721 gtgatttgca gtgccgagcc gaagcataaa cagcgccaag gctgattttt tcgcatattt
    12781 ttcttgcaaa gttgggttga gctggctaga ttagccagcc aatcttttgt atgtctgtgc
    12841 gtttccattt gagtatcctg aaaacgggct tttcagcatg gaacgtacat attaaatagt
    12901 aggagtgcat agtggcccgt atagcaggca ttaacattcc tgatcataag catgccgtaa
    12961 tcgcattaac ttcgatttat ggcgtcggca agacccgttc taaagccatc ctggctgcag
    13021 cgggtatcgc tgaagatgtt aagatcagtg agctgtctga aggacaaatc gacacgctgc
    13081 gtgacgaagt tgccaaattt gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca
    13141 tcaagcgcct gatggatctt ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg
    13201 ttcgcggtca gcgtaccaag accaacgcac gtacccgtaa gggtccgcgc aaaccgatca
    13261 agaaataatc ggggtgattg aataatggca aaggcaccaa ttcgtgcacg taaacgtgta
    13321 agaaaacaag tctctgacgg cgtggctcat atccatgctt ctttcaacaa caccatcgtg
    13381 actatcactg atcgtcaggg taacgcgttg ggttgggcaa cagccggtgg ttccggtttc
    13441 cgtggttctc gcaaatccac tccgtttgca gcacaggttg cagcagagcg ttgcgctgac
    13501 gtcgtgaaag aatacggcat caagaatctg gaagttatgg ttaaaggtcc gggtccaggc
    13561 cgcgaatcta ctattcgtgc tctgaacgcc gcaggtttcc gcatcactaa cattactgat
    13621 gtgactccga tccctcataa cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc
    13681 gttttccagg tttgttggag aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg
    13741 agccgtcgtg agggcaccga cttattcctt aagtctggcg ttcgcgcgat cgataccaag
    13801 tgtaaaattg aacaagctcc tggccagcac ggtgcgcgta aaccgcgtct gtctgactat
    13861 ggtgtgcagt tgcgtgaaaa gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag
    13921 ttccgtaact actacaaaga agcagcacgt ctgaaaggca acaccggtga aaacctgttg
    13981 gctctgctgg aaggtcgtct ggacaacgtt gtataccgta tgggcttcgg tgccactcgt
    14041 gcagaagcac gtcagctggt tagccataaa gcaattatgg taaacggtcg tgttgttaac
    14101 atcgcttctt atcaggttag tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag
    14161 cagtctcgcg tgaaagccgc tctggagctg gctgagcagc gtgaaaagcc aacctggctg
    14221 gaagttgatg ctggcaagat ggaaggtacg tttaagcgta agccggagcg ttctgatctg
    14281 tctgcggaca ttaacgaaca cctgatcgtc gagctttact ccaagtaaag cttagtacca
    14341 aagagaggac acaatgcagg gttctgtgac agagtttcta aaaccgcgcc tggttgatat
    14401 cgagcaagtg agttcgacgc acgccaaggt gacccttgag cctttagagc gtggctttgg
    14461 ccatactctg ggtaacgcac tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt
    14521 gaccgaggtt gagattgatg gtgtactaca tgagtacagc accaaagaag gcgttcagga
    14581 agatatcctg gaaatcctgc tcaacctgaa agggctggcg gtgagagttc agggcaaaga
    14641 tgaagttatt cttaccttga ataaatctgg cattggccct gtgactgcag ccgatatcac
    14701 ccacgacggt gatgtcgaaa tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga
    14761 gaacgcgtct attagcatgc gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc
    14821 tacccgaatt cattcggaag aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg
    14881 ctacagccct gtggagcgta ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac
    14941 cgacctggac aagctggtca tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc
    15001 gattcgtcgt gcggcaacca ttctggctga acaactggaa gctttcgttg acttacgtga
    15061 tgtacgtcag cctgaagtga aagaagagaa accagagttc gatccgatcc tgctgcgccc
    15121 tgttgacgat ctggaattga ctgtccgctc tgctaactgc cttaaagcag aagctatcca
    15181 ctatatcggt gatctggtac agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg
    15241 taaaaaatct cttactgaga ttaaagacgt gctggcttcc cgtggactgt ctctgggcat
    15301 gcgcctggaa aactggccac cggcaagcat cgctgacgag taaccggatc acaggttaag
    15361 gttttactga gaaggataag gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca
    15421 acagcagcca tcgccaggct atgttccgca atatggcagg ttcactggtt cgtcatgaaa
    15481 tcatcaagac gactctgcct aaagcgaaag agctgcgccg cgtagttgag ccgctgatta
    15541 ctcttgccaa gactgatagc gttgctaatc gtcgtctggc attcgcccgt actcgtgata
    15601 acgagatcgt ggcaaaactg tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg
    15661 gttacactcg tattctgaag tgtggcttcc gtgcaggcga caatgcgccg atggcttaca
    15721 tcgagctggt tgatcgttca gagaaagcag aagctgctgc agagtaatct gaagcaacgt
    15781 aaaaaaaccc gccccggcgg gtttttttat acccgtagca tccccactta tctacaatag
    15841 ctgtactctt tttgttcatc ccctggagta tttatgtggt tacttgacca gtgggcagag
    15901 cgtcatatag cagaagcgca agcgaaaggt gagtttgata acctggtagg tagcggcgaa
    15961 ccattgatac tggatgatga ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg
    16021 ctgaagaatg ctggttgctt accgccagaa cttgagcata ggagggaagc aatacagctt
    16081 ctggatattc tcaaaggtat ccgtcatgat gatccgcaat atcaagaggt tagtcgtcga
    16141 ttgtcattac tggaattgaa gctgcgacaa gctggattga gtaccgattt tttacgcggc
    16201 gattatgctg acaagttgtt gaacaaaatc aacgataact agtggagtat gtatgtatcg
    16261 cattggtgag ctggcaaaaa tggcggaagt aacacccgac acgattcgtt attacgaaaa
    16321 gcagcagatg atggagcatg aagtacgcac cgaaggtggg tttcgcctgt ataccgaaag
    16381 cgatctccag cgattgaaat ttatccgcca tgccagacaa ctaggtttca gtctggagtc
    16441 gatccgcgag ttgctgtcga tccgtatcga tcctgaacac catacctgtc aggagtcaaa
    16501 aggcattgtg caggaaagat tgcaggaagt cgaagctcgt atagccgagt tgcagagtat
    16561 gcagcgttcc ttgcaacgcc ttaatgatac ctgttgtggg accgcccata gcagtgttta
    16621 ttgctcgatt cttgaagctc ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt
    16681 ttttgcactg gcaggattac actcgcgccg ttaaataacc aattggagtt tttatgagcc
    16741 gatatcagca tactaaaggg cagataaagg ataatgcgat agaagcatta ctacatgatc
    16801 ccttattccg acagcgagta gagaaaaata agaaagggaa aggcagttac atgcgaaaag
    16861 gaaaacatgg caatcggggg aactgggagg ccagtggcaa gaaagtaaat cactttttta
    16921 ccactggcct tctgctttca gacgcttgtt aagagcggtt attctgctct ttcagcaaat
    16981 cacgaatttc tgtcagtaat acttcttctt tagttggtgc aggtgttgct gctgctggtt
    17041 cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg gcaaaggcca
    17101 ctatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca acagcaggga
    17161 tatccccttg ggcctcgcgt agcgtgacag caaactgttt aaagtcgatc ccgccaatta
    17221 ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc ttcccgaatg
    17281 ccgctccgat aatgacaccc actgccaaat ccaccacgtt cccgcgcatc gcaaattcgc
    17341 gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca taagtctaac
    17401 aaatgttaag ccattttcct gccaggaatg gatttaatta atccttaata atcaaaggga
    17461 aataataagg cgtcattaga cgccttatta attacaagaa gaaaggactt ggctggaaga
    17521 gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg tgatcgcctt
    17581 gctcaatgcg cagattgtca ttggcaatca tcacatcgtt tccacgtacc accgctccaa
    17641 taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg cgtgaggtgc
    17701 tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgt aatgaggaaa
    17761 caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt gcttgttgtg
    17821 gtgaaatcgc aatatcgatc acgctcccct gaaccagatc cacataagcg cgacgttgga
    17881 tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac atgatattgg
    17941 cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt tcttcggcca
    18001 gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac ttttccgcca
    18061 gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct ttttccagac
    18121 gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc ttatacggtt
    18181 tttccagtcg ctgtaattca ctcatcaccg cacggatatg ctgtgaagcg gcaataaaga
    18241 acacttcatc accagcttca acaatagtcg aaccttgcgg acgaatgggg cgatcgtggc
    18301 ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg
    18361 cattaccaat cagcgggccg ccataatagg ctttaaccac agccaggctg actttaccct
    18421 cagcgaagtt caccacctgc aatgcgccgg gatactcaat cagtcggtaa atattatcga
    18481 taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa tgaaatagtt
    18541 tatcggcatc gcgcacgtag tctggtgagc ggatacgagc gatgcgatta ggggtgttga
    18601 aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg gttacagcaa
    18661 ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga gagccatgcc
    18721 cctgcacgac ccgcaggtca aatttatcct gtaaggtccg cagacgctca ccgttggtat
    18781 cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct
    18841 ggccggcacc cagaatgata attttcatca gtcgcgaccc gttatctcat cactttttga
    18901 ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc gggcaaattt tgtttacccg
    18961 gttgctctgg tgttcctgtt tcacaaagtt cggcatcagc ggtacgttgc aaaaaggctt
    19021 taatctgcag gctattttct tccggtaaca ccgaacaggt ggcatagacc agggttccac
    19081 cggattttaa atgtggccaa atggcatcga gtatttcaga ctgcaattgc gcgagttccg
    19141 ggatatcgcg atcacgacgt aaccatttaa tatccggatg gcgacgaatc acccccgttg
    19201 ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac cattgggaag
    19261 ggttgcggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt aaattgtcgt
    19321 aaacgcgaga gaggcgctgc tcatcaatat cgaccgccag aacttgcgcc tctggagcaa
    19381 cctcaagaat atgcgttgtt ttaccgcctg gggcggcgca aagatccaga atgtgttcac
    19441 cattttgtgg agcaagccag gtcatgcaac cttgtgctga agcatcctga acggtaaccc
    19501 atccttcttc aaaaccgggt agcgcatgaa caggtgcagg tgtttccagc tgtacagcat
    19561 cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccagcaat gcaagccagc
    19621 tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc tggttattgg
    19681 cttcgacgat ggattgccac tgctcgggat acgccttttg cagacgcttc agcaaccagg
    19741 aaggatgcag ataacgtgca tcactggcat taaactcggc taataactca tcttgctgac
    19801 gctggaactg acgtaataca ccgttaatca atcccttaag ttgcgggcgc ttaattgcaa
    19861 ctgcgccttc aaccgtttca gccagcgcgg catgaggtgg aatgcgggta taaagcagtt
    19921 gatacaaacc aaccataatc aggtaatgaa cagtacgctg tttgcctgtc atcggacggg
    19981 ccattagctt attaatcagc cagtctaact gtgagagcgt acgcagtacg ccaaagcata
    20041 actcctggag aagagcttta tctttatcgg aaactttttg ctggagcggt ggcagaatgt
    20101 tgctcagtga ttgcccttgc tcaacgactt gttcaatggc ctgggccgcc atgctacgta
    20161 aattacgttg ttttttcata agtataaata taaagatgcc cggtaagacc gggcttagaa
    20221 gagtggacta tcaggccaga cggttgcccg gtacaaacca ttcccgacga gaattcagga
    20281 ggtcttgcac gctcatcgct ttcttacctg caggttgcat tgagagcaga ttcaggatgc
    20341 catcacctgt cgctacctga atgccttgtt tattggcttc aaggattgtt cctggcgcag
    20401 cctttgttgt cgtatcgatg acagatgctt tccagacttt aaccggctgc ccttcaattt
    20461 ccagccagct cattggccat ggattgaaag cgcgaatgca acgttcaagc tgtgctgccg
    20521 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ttcggcgtaa gtgacaagag
    20581 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa
    20641 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
    20701 taatcgggca ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca
    20761 taatggtcac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgctgcac
    20821 cgcgccagcg tggcagcagt gaaccatgaa cgttgataca gccaagacgc ggcatctcca
    20881 gcacagcttt aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcagat
    20941 cggcgaccag ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac
    21001 ctttgtcctc agccagaact ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg
    21061 gtcggtctgg ctgggtgaac acgccaacga tgttatgacc agaagacaac agcgcgtcga
    21121 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
    21181 taattcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
    21241 acgttgttgt ttcagcggtg acagataatc cataaacagt ttgccgacca ggtgatccat
    21301 ctcatgctga atacagatgg ctaacagacc gtctgcttcc agttcaaatg gtttaccgtc
    21361 gcggtccagg gcgcgaattt taactttctc tgcgcgtggc actaaagcac gttgttcagg
    21421 gatcgacagg caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
    21481 gattaacacc agccgttcgt cacggttttc cgaaacatca attacaatga tacgttgatg
    21541 gatatcaacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
    21601 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
    21661 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
    21721 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
    21781 cactgaccaa tcgcaaagat tgctaaggct gcttatggca gggagataag gatggtcgat
    21841 acagaaattt ggctgcgttt aatgagcatc agcagcttgt atggcgatga tatggtccgt
    21901 atagctcact ggctggcaaa acagtcgcat attgatgcgg ttgtattgca gcaaacaggg
    21961 cttacattgc ggcaggcaca acgctttctt tcatttccgc gaaagagtat cgaaagctca
    22021 ctttgttggt tggagcaacc caaccatcat ttaatccctg cggacagcga attttatcct
    22081 cctcaacttc tggcgacgac agattacccc ggcgcactgt ttgttgaagg agaactgcac
    22141 gcgttgcatt catttcagct tgccgtagtg gggagtcggg cgcattcatg gtatggcgag
    22201 cgatggggac gattattttg cgaaactctg gcgacgcgtg gagtgacaat tacgagtgga
    22261 ctggcgcgtg gaatcgatgg tgtggcgcat aaagcggcct tacaggtaaa tggcgttagc
    22321 attgctgtat tggggaatgg acttaatacc attcatcccc gccgccatgc tccactggct
    22381 gccagtctac ttgagcaagg tggtgctctc gtctcggaat ttcccctcga tgttccaccc
    22441 cttgcttaca atttcccacg aagaaatcgc attatcggtg gtctaagtaa gggtgtactg
    22501 gtggtggaag cggctttgcg cagtggttcg ctggtgacag cacgttgtgc gcttgagcag
    22561 gggcgtgaag tttttgcttt gccaggtcca atagggaatc cgggaagcga agggcctcac
    22621 tggttaataa aacaaggtgc gattcttgtg acggaaccgg aagaaattct ggaaaacttg
    22681 caatttggat tgcactggtt gccagacgcc cctgaaaatt cattttattc accagatcag
    22741 gaagacgtgg cattgccatt tcctgagctc ctggctaacg taggagatga ggtaacacct
    22801 gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag aggtagttac tcaactactc
    22861 gaactggagt tagcaggatg gatcgcagct gtacccggcg gctatgtccg attgaggagg
    22921 gcatgccatg ttcgacgtac taatgtattt gtttgaaacc tatattcaca cagaagctga
    22981 gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc gacgcaggat ttgatcgaga
    23041 agatatctac aatgccctgc tttggctgga aaagcttgcg gattatcagg aaggactggc
    23101 agaaccgatg caactggcct ctgatccact ctccatgcgt atttataccc ctgaagagtg
    23161 tgaaagactg gatgccagct gtcgtggttt tttgcttttc cttgagcaaa ttcaggtgct
    23221 tagccttgaa acccgtgaaa tggtgataga gcgagtgctc gcgctggata ccgcagagtt
    23281 cgacctggaa gacctgaaat gggtaatcct gatggtgttg ttcaatattc cgggttgcga
    23341 aaatgcgtac cagcaaatgg aagaattact ctttgaagtg aatgaaggta tgctgcatta
    23401 atcatttctt taattcagca taagttgtta tggcgaaatc agcactgttc acggtgcgta
    23461 ataatgagtc ctgcccaaag tgcggggctg aactagttat tcgatccggg aaacacggtc
    23521 cgtttcttgg atgctcacag tatccggcat gtggctacgt ccgtcctctg aaatcttcag
    23581 cggatggaca tattgtcaaa gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc
    23641 tggtattacg ccagggacgc tttggtatgt ttattggttg cagtaactac cctgaatgcg
    23701 aacataccga acttatcgat aaaccggacg aaacagcaat tacatgcccc caatgccgga
    23761 cgggccatct ggttcagcgc cgctcccgtt atggcaaaac atttcactcc tgtgatcgct
    23821 acccggagtg tcaatttgcc attaacttca aacccatagc tggagaatgc cctgagtgcc
    23881 attatccgct actcatcgaa aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta
    23941 aacaatgtgg aaagccgatt tcggcggaat aataacgtga ataataacct gcaaggagac
    24001 gctatcgcag ctgcgataga tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa
    24061 gccgttttcg gtgtcgggtg cgatcctgat agcgaaacag cagtgatgag actgttggag
    24121 ttaaaacggc gtccggttga taaggggctg attttaatcg cggcaaatta cgagcagctt
    24181 aaaccctata ttgatgacac catgctgact gacgcgcagc gtgaaaccat tttttcccgc
    24241 tggccaggtc ctgtaacctt tgtctttccc gcgcctgcga caacaccgcg ctggttgacg
    24301 ggccgctttg attcgcttgc tgtacgagtc accgaccatc cgttggtggt tgctttgtgc
    24361 caggcttatg gtaaaccgct ggtttctacc agtgccaact tgagtggatt gccaccttgt
    24421 cgaacagtag acgaagttcg cgcacaattt ggcgctgcgt tcccggttgt gcctggtgaa
    24481 acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc tgacgggtga actgtttcga
    24541 caggggtaac ataatggaaa cctatgctgt ttttggtaat ccgatagccc acagcaaatc
    24601 gccattcatt catcagcaat ttgctcagca actgaatatt gaacatccct atgggcgtgt
    24661 gttgacaccc atcaatgatt tcatcaacac actgaacact ttctttagtg ctggtggtaa
    24721 aggtgcgaat gtgacggtgc cttttaaaga agaggctttt gccagagcgg atgagcttac
    24781 tgaacgggca gcgttggctg gtgctgttaa taccctcaag cggttagaag atggacgctt
    24841 gctgggtgac aataccgatg gtataggttt gttaagcgat ctggaacgtc tgtcttttat
    24901 ccgtcctggt ttacgtattt tgctcatcgg cgctggtgga gcatctcgcg gcgtactact
    24961 gccactcctt tccctggact gtgcggtgac aatcactaat cggacggtat cccgcgcgga
    25021 agagttggct aaattgtttg cgcacactgg cagtattcac gcgttgggta tgcacgaact
    25081 ggaaggtcat gagtttgatc tcattattaa tgcaacatcc agtggcatca gtggtgatat
    25141 tccggcgatc ccgtcatcgc tcattcatcc aggcatttat tgctatgaca tgttctatca
    25201 gaaaggaaaa actccttttc tggcatggtg tgagcagcga ggctcaaagc gtactgctga
    25261 tggtttagga atgctggtgg cacaagcggc tcatgccttc cttctttggc atggcgttct
    25321 gcctgatgta gaaccggtca tcaaactatt gcaacaggag ttgtcagcgt gaatcaggcc
    25381 atccagtttc cggataggga agagtgggac gagaataaaa aatgtgtatg ttttcccgct
    25441 ctcgtgaatg gtatgcaact gacatgtgcg atctctggcg agagtctggc gtatcgcttt
    25501 actggagata caccagaaca gtggttagag agttttcgtc agcatcgctg ggacctggaa
    25561 gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg atcaaggttg ggtgtggtta
    25621 ccctgatcca tatattcgtc cttccatttc acgtagttat ttgcggaata gcgtaaccca
    25681 gccttctctt catcacttaa cgggcggatc tgtttgacgg ggctaccgag atacagatat
    25741 ccgctctcca gccgtttatt ttgtgggacc aggctacctg caccaatcat cacatcatct
    25801 tctactattg cgccatcaag taaaattgag cccatcccaa ccaaaactcg attgccaatg
    25861 gtgcagccgt ggagcatcac cttgtgacca atagtgacat cttcgccaat ggttaatggg
    25921 ttgccagctg ggttgtacga ggatttatga gtgacgtgca acatactgcc atcctggata
    25981 ttggtgcgtg ctccgatctg tacataatgt acatctccac gaatcacaac gagcggccag
    26041 atccccacat catcagccag acgaacgtca ccaatcacga cgctgctatc gtcgatcatt
    26101 acgcgctgac cgatttgtgg aaaaagatcg cggtatgggc gtaaaacctc agacatactc
    26161 acctcaacaa taaatgattt actaatgact ttgggtacat tattggcctt gtgcaagtct
    26221 tttagtatgc aaaaaagcac cgttttgtgt gcgaatgcag caaaaagggt gaaaaaacaa
    26281 caaacagaaa aaaagatcaa aaaagtactt gtgcaaaaaa ttgggatccc tataatgcgc
    26341 ctccgttgag acgacaacgt gaaacacttc acaagatagt cgtaacaaca aagagaaaaa
    26401 tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag
    26461 tgagctgaaa accgcgtcgc aactgctctt taacaattta tcagacaatc tgtgtgggca
    26521 ctcgaagata cggattcttg acgtcgcaag acgaaaaatg aataccaagt ctcaagagtg
    26581 aacacgtaat tcattacgaa gtttaattca cgagcatcaa acttttaaat tgaagagttt
    26641 gatcatggct cagattgaac gctggcggca ggcctaacac atgcaagtcg aacggtaaca
    26701 ggaagcagct tgctgctttg ctgacgagtg gcggacgggt gagtaatgtc tgggaaactg
    26761 cccgatggag ggggataact actggaaacg gtagctaata ccgcataacg tcgcaagacc
    26821 aaagaggggg accttagggc ctcttgccat cggatgtgcc cagatgggat tagctagtag
    26881 gtggggtaa
//
LOCUS       DAJOTB010000057        26478 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJOTB010000057 DAJOTB010000000
VERSION     DAJOTB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.057"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            113..2341
                     /gene="crfC"
                     /locus_tag="OHN28_002342"
     CDS             113..2341
                     /gene="crfC"
                     /locus_tag="OHN28_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCQ2297735.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2338..3216
                     /locus_tag="OHN28_002343"
     CDS             2338..3216
                     /locus_tag="OHN28_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCQ2297736.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3480..4982
                     /gene="proP"
                     /locus_tag="OHN28_002344"
     CDS             3480..4982
                     /gene="proP"
                     /locus_tag="OHN28_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCQ2297737.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5094..5183
                     /gene="pmrR"
                     /locus_tag="OHN28_002345"
     CDS             5094..5183
                     /gene="pmrR"
                     /locus_tag="OHN28_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCQ2297738.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5159..6259)
                     /gene="pmrB"
                     /locus_tag="OHN28_002346"
     CDS             complement(5159..6259)
                     /gene="pmrB"
                     /locus_tag="OHN28_002346"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCQ2297739.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(6260..6928)
                     /gene="basR"
                     /locus_tag="OHN28_002347"
     CDS             complement(6260..6928)
                     /gene="basR"
                     /locus_tag="OHN28_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCQ2297740.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6925..8568)
                     /gene="eptA"
                     /locus_tag="OHN28_002348"
     CDS             complement(6925..8568)
                     /gene="eptA"
                     /locus_tag="OHN28_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCQ2297741.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8672..10009)
                     /gene="adiC"
                     /locus_tag="OHN28_002349"
     CDS             complement(8672..10009)
                     /gene="adiC"
                     /locus_tag="OHN28_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCQ2297742.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10146..10907)
                     /gene="adiY"
                     /locus_tag="OHN28_002350"
     CDS             complement(10146..10907)
                     /gene="adiY"
                     /locus_tag="OHN28_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCQ2297743.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11243..13510)
                     /gene="adiA"
                     /locus_tag="OHN28_002351"
     CDS             complement(11243..13510)
                     /gene="adiA"
                     /locus_tag="OHN28_002351"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCQ2297744.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13709..14617)
                     /gene="melR"
                     /locus_tag="OHN28_002352"
     CDS             complement(13709..14617)
                     /gene="melR"
                     /locus_tag="OHN28_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCQ2297745.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAILNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14900..16255
                     /gene="melA"
                     /locus_tag="OHN28_002353"
     CDS             14900..16255
                     /gene="melA"
                     /locus_tag="OHN28_002353"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2297746.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEDYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(16252..16902)
                     /locus_tag="OHN28_002354"
     CDS             complement(16252..16902)
                     /locus_tag="OHN28_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCQ2297747.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWLAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(17025..18671)
                     /gene="fumB"
                     /locus_tag="OHN28_002355"
     CDS             complement(17025..18671)
                     /gene="fumB"
                     /locus_tag="OHN28_002355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2297748.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(18749..20089)
                     /gene="dcuB"
                     /locus_tag="OHN28_002356"
     CDS             complement(18749..20089)
                     /gene="dcuB"
                     /locus_tag="OHN28_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCQ2297749.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(20660..21379)
                     /gene="dcuR"
                     /locus_tag="OHN28_002357"
     CDS             complement(20660..21379)
                     /gene="dcuR"
                     /locus_tag="OHN28_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCQ2297750.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(21376..23007)
                     /gene="dcuS"
                     /locus_tag="OHN28_002358"
     CDS             complement(21376..23007)
                     /gene="dcuS"
                     /locus_tag="OHN28_002358"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCQ2297751.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKAQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            23188..23418
                     /locus_tag="OHN28_002359"
     CDS             23188..23418
                     /locus_tag="OHN28_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCQ2297752.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            23430..23702
                     /locus_tag="OHN28_002360"
     CDS             23430..23702
                     /locus_tag="OHN28_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2297753.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKIREYDDIRS"
     gene            complement(23812..26349)
                     /locus_tag="OHN28_002361"
     CDS             complement(23812..26349)
                     /locus_tag="OHN28_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2297754.1"
                     /translation="MKGSLKKSLLLLRITIICPVFSCAAVSPSPDNSHRFSFNPDFLQ
                     LSDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIKIDFFAQDNKLNARLTP
                     QQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQKLILNIPQSYLKPQDWL
                     STPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSSLNLGGWRLRHNGNWST
                     NSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDSFPFEGAQFSSDDGMIA
                     PELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELRDFNQIFTGDLEVEIRE
                     ADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNSVSEPQFVQSSAAIGLP
                     YEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQFSQNYKSFGKQQGQSY
                     RFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVDEKNINSYHQRSRISTT
                     ISQDLQDWGQLYLSASKDQYWDTADGYNLSASYSLPFRYISAMLSLGYNKSPYYHEAD
                     KSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGSSQDGEFNYAVAQGWQN
                     QNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGITLHPHGLTLSQPLSLDS
                     ASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQISLDVNSVKDNVELLNT
                     DVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVVTLDSENSINSSIVADQ
                     GQVYMSGLPEEDTLIAQWGEEASQKCKTNYKLQNKKAKFSELTLQCQ"
BASE COUNT         6585 a         6321 c         6762 g         6810 t
ORIGIN      
        1 acacgctaaa tataaaatta tttcctgcag taattataaa agccgaaaac agaactcaaa
       61 aacaatctgg ctaaataaaa gtaataaaat ttgctttaag gaagaatatt ctatgtacac
      121 acagaccctg tatgagttaa gtcaggaggc tgaacgcctg ttacagcttt cccgacaaca
      181 gttgcagtta ctggaaaaaa tacctctctc tgtacccgga gacgatgcgc cacaactggc
      241 tttaccctgg agtcagccta atatcgccga acgtcacgcg atgctgaata atgagttgcg
      301 taaaatttcc cggctggaaa tggtgctggc gattgtcggt accatgaaag cagggaaatc
      361 aaccaccatt aatgccattg ttggtacgga ggttctgcct aatcgcaatc gcccaatgac
      421 tgcgctgcca acgcttattc gccatacgcc cgggcaaaaa gaaccggtac tgcatttttc
      481 acatgtcgcg ccaatcgacc atttaattca acaattacaa cagcgcctgc gtgattgcga
      541 tattaagcat ctgaccgatg tgctggaaat agataaagat atgcgtgcgc ttatgcagcg
      601 gatcgaaaat ggcatcgctt tcgaaaaata ttatctgggt gctcaaccta tttttcattg
      661 tctgaaaagt ttgaatgatt tggtgcgact ggcgaaggcg ctggacgtcg attttccttt
      721 ttctgcttac gccgccattg agcatattcc cgtgattgaa gtggagtttg tccatctggc
      781 ggggctggag agttatcccg gtcagttgac gttactggat acccccgggc caaatgaagc
      841 cgggcaaccg catctgcaaa aaatgcttaa ccagcagctg gcacgcgcct cggcggtact
      901 ggcggtgctg gattatacgc aactgaaatc gatctccgat gaagaggtcc gtgaggcaat
      961 tttggcggtg gggcaatcgg tgccgttgta tgtgctggtc aataagttcg atcaacagga
     1021 tcgtaacagt gacgacgccg accaggtgcg ggcactgatt tccggaacgc tgatgaaagg
     1081 ctgtattacg ccacagcaga tatttccggt gtcgtcgatg tggggctacc tggcgaatcg
     1141 ggcgcgccat gagttagcca acaacggtaa gttacccgcg ccagagcaac aacgctgggt
     1201 ggaagatttt gcccatgccg cgctcggcag gcgctggcgt catgccgatc tggcggacct
     1261 cgaacatatt cgccatgctg ccgatcagtt gtgggaagat tcgctgttcg cccagccaat
     1321 tcaggcgttg cttcatgccg cttacgctaa cgcctcgttg tatgctctgc gatctgccgc
     1381 gcataaactg ttgaattacg cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg
     1441 gttaaacgtc gcttgtgaac aattgcggca aaatatccat caggtcgaag aaagtttgca
     1501 gctattgcaa ctcaatcagg cgcaggtgag cggcgagatt aaacatgaaa tcgagttggc
     1561 cctgacctcc gccaaccact ttctgcgtca acagcaagat gcggtgaatg cgcagttagc
     1621 cgcgttgttt caggataatt cggagtcgtt aagtgagatg cgtacccgct gtgagacact
     1681 gttacagacg gcgcagaaca ccatcagtcg cgactttacg ctgcgctttg ccgagcttga
     1741 gtccaccctt tgccgggtgt taaccgatgt tattcgcccc attgagcaac aagtcaaaat
     1801 ggaattgagt gagtctgggt ttcgtcctgg gtttcatttt cctgtttttc acggcgtagt
     1861 tccccacttc aacacccgcc agctgttcag tgaagtcatt tcgcgccagg aagcaacgga
     1921 cgagcagagc acgcgtttag gcgttgtgcg tgagattttt tcgcgctggt tgaatcagcc
     1981 cgactgggga cggggaaatg agaaattccc gacagagacg gttgattaca gtgtgttgca
     2041 acgagcatta agcgcagaag tcgatcttta ttgccaacaa atggctaaag ttatggcaga
     2101 gcaggtcgat gaatctgtta cggcaggcat gaatactttt ttcgctgagt tcgcttcatg
     2161 tttgacggaa ttacagacgc gtttacgcga aagtatggct ctgcgtcaac aaaatgaatc
     2221 ggtggtcagg ctgatgcaac agcaattgca gcagactgtg atgactcacg gctggattta
     2281 caccgacgct cagctgttac gcgatgatat tcaaacactt ttcacggcag aacgatattg
     2341 accaagacgt tactggacgg ccccggtcgc gtgctggagt cggttcatcc gcgcttttta
     2401 gtggatctgg cgcagggcga tgatgcccgc cttccacaag cccatcagca gcagtttcgt
     2461 gaacgactga tgcaggaact tcttgcgcgc gtgcagcttc agacatggac gaacggcggc
     2521 atgttaaatg cgccgcttag cctgcgtctg acattagtgg aaaaactggc gtcgatgctg
     2581 gatcccggtc atctggcgct gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg
     2641 gatcaccgcc agcactctgc tttcccggag ctcccccagc aaattgtcgc cttgtatgag
     2701 tggttttcag cccgttgtcg ctggaaggaa aaggcgttaa cgcaacgagg cctattggtg
     2761 caggcgggtg aacagagcga gcaaattttt acccgctggc gtgctggggc gtataacgcc
     2821 tggtcgttgc ctgggcgctg ttttatcgtt ctcgaagagt tgcgctgggg tgcatttggc
     2881 gatgcctgcc gtctgggaag cccgcaagcg gtggcgttgt tgctaggtga tttgcgcgtg
     2941 aaagcgacac aacatttggc agagagtatc aatgcggccc cgaccacgcg tcactattac
     3001 catcagtggt ttgcctcttc gaccgtttcg acgggcgggg atcatgctga ttttttaagt
     3061 tggctgggaa agtggtctac ggcagataaa caacccgttt gctggtcagt gacccaacgc
     3121 tggcgaactg tcgcactggg gatgccacga ctctgttcag cgcagcgtct ggcgggggca
     3181 atggtcgagg aaatcttctc tgtaaatttg gtgtaaataa tcagttacac caatgagtcc
     3241 taaacgaaat gcatgtgtga agttgatcac aaatttaaac actggtaggg taaaaaggtc
     3301 attaactgcc caattcaggc gtcaactggt ttgattgtac attccttaac cggagggtgt
     3361 aagcaaaccc gctacgcttg ttacagagat tgcatcctgc aattcccgct ccccttttgc
     3421 ggccgtcgcg ctgatttttc tggcgtttgc ggaaatgggc caactctgcg aggaaagcta
     3481 tgctgaaaag gaaaaaagta aaaccgatta cccttcgtga tgtcaccatt attgatgatg
     3541 gtaaactgcg taaagccatt accgcagcat cactgggtaa tgcaatggaa tggttcgatt
     3601 ttggtgttta tggttttgtt gcttacgcat taggtaaagt ttttttcccg ggggctgacc
     3661 ccagcgtgca gatggttgct gcactcgcca ctttctccgt tccctttctg attcgaccgc
     3721 ttggcgggct cttctttggt atgttgggcg ataaatatgg tcgccagaag atcctcgcta
     3781 tcactattgt gattatgtcg atcagtacgt tctgtattgg cttaataccg tcctacgaca
     3841 cgattggtat ttgggcaccg attctgctgt tgatctgtaa gatggcacaa ggtttctcgg
     3901 tcggcggtga atataccggg gcgtcaatat ttgttgcgga atactcccct gaccgtaaac
     3961 gtggctttat gggcagctgg ctggactttg gttctattgc cgggtttgtg ctgggagctg
     4021 gcgtagtggt gttaatttcg accattgtcg gcgaagagaa cttcctcgat tggggctggc
     4081 gtattccgtt ctttatcgct ctgccgttag ggattatcgg gctttacctg cgccatgcgc
     4141 tggaagagac tccggcgttc cagcagcatg tcgataaact ggaacagggc gaccgcgaag
     4201 gtttgcagga tggcccgaaa gtctcgttta aagagattgc caccaaacac tggcgcagcc
     4261 tgttgacatg tattggtctg gtaattgcca ccaacgtgac ttactacatg ttgctgacct
     4321 atatgccgag ttatttgtcg cataacctgc attactccga agaccacggg gtgctgatta
     4381 ttatcgccat tatgatcggt atgctgtttg tccagccggt gatgggcttg ctgagtgacc
     4441 gttttggccg tcgtccgttt gtgctacttg gtagtgttgc actgtttgtg ttggcgatcc
     4501 cggcgtttat tctgattaac agtaacgtca tcggcctgat ttttgccggg ttactgatgc
     4561 tggcggtgat ccttaactgc tttacgggcg ttatggcttc taccttgcca gcgatgttcc
     4621 cgacgcatat ccgatacagc gcgctggcgg cggcatttaa tatttcggtg ctggttgccg
     4681 gtctgacgcc aacactggcg gcctggctgg tcgaaagctc gcagaatctg atgatgcctg
     4741 cctattacct gatggtagtg gcggtggttg gtttaatcac cggcgtaacc atgaaagaga
     4801 cggcaaatcg tccgttgaaa ggtgcgacac cggcggcgtc agatatacag gaagcgaagg
     4861 aaattctcgt cgagcattac gataatatcg agcagaaaat cgatgatatt gaccacgaga
     4921 ttgccgattt gcaggcgaaa cgtacccgcc tggtgcagca acatccgcga attgatgaat
     4981 aagctgaaac ggatggcctg atgtgatgct gtcttatcag gtcaattgaa ctcttaaggt
     5041 tcacttaatc tctgacgcgc atactctcct ccaggttaac ggaggagagt gcaatgaaaa
     5101 accgtgttta tgaaagttta actaccgtgt tcagcgtgct ggtggtcagc agctttcttt
     5161 atatctggtt tgccacgtac tgatcttttt tcagccgtat ccaggcccgc gtgccggaag
     5221 tctcttgtcg gttttgcagg aaaaactgcc cgtgatgcaa ctgtgtaatg cggctgacaa
     5281 tacttaaccc cagaccaatt ccgccataac ggctgtccat tcgtacaaat gctttactca
     5341 actccccgca tttactctca tcaatacccg gtccttcatc ttcaacggcc atgaccgctc
     5401 cgccatcttc ttgcagctta atcataatgt tgctgccttg cgggctataa cgatgggcgt
     5461 tttctaccag gtttcgcaat aacatccgca gcagggttgc atcaccctga acggtgatgt
     5521 cggcggcgct ctctggcaat agcagggttt gctggcgctg gtcgagcatg gtactgagtt
     5581 cgtcatacga ggggagaatg acatcttcca gcagttttac atgttgataa ttaccggaag
     5641 aaaatgactg tccggcacgc gccagttgca gcaactggga gacgctctcc atcatctgat
     5701 caagtcgtgc cactaacggt gctacatcaa tgtgatgcgt tttcgccagc agttccagat
     5761 gcaaacgcac ccccgccagt ggcgttcgca gttcgtgcgc gacgtcagcg gtaaacaacc
     5821 tttcattatc gagcgtgctg gtcaggcgac tgaccagatc gtttaacgcc gaaaccaccg
     5881 catcgatttc gagggtggcg ctatgaatgg caatgggcgt taagttgtcg gcggtgcgcg
     5941 cttccagctc tttttgcagc tccgccagcg gtcgggtgat gcggcgtacc gcctgatagc
     6001 agataaatag cgtcaggcta accataaaga cgccggggac aatcaggctg gcgaccgcct
     6061 cgcggatctc acgcatgatg tggcgatcgt tgttgcgatt gtcgcgtagc gcctgctcaa
     6121 acagctgaat ctgctcggta ctttcatgcc atagccagaa gacgctgatc agctcaaaca
     6181 ccaacaaaat ggccccgatg gtcaatatca gccgttggcg cagcgatatt ggtcggcgca
     6241 gaaaacgcat cagattcaat tagttttcct catttgcgac cagcatatag ccaaatccgc
     6301 gcacggtgcg gatacgggct ttgcccactt tgtcgcgcag gttatggata tgcacttcca
     6361 gggtgttggt cgagggctcg ttgtcccagt tgtagatatc gttgtagaga atttcccgat
     6421 gcaccgggct gcctgcttta agcatcaggc gcgacagcag agcatattct ttgggcgtca
     6481 gaatcaactc ttcaccgccc atccagacct gacggcgacc catattcagc gtcaggttgc
     6541 caacaatcag ctcactttcg ccctgattat tatggcgtcg tagcagggcg cggatacggg
     6601 cgtgcaactc ttccagcgca aaaggcttca ccagataatc gtcggccccg acatccagcc
     6661 cggcgatttt gtcggtaagg gtatcgcggg cggtgagaat taacaccggc agagtgtatt
     6721 ttttctgccg gatacgggca agaaaatgca gcccatcttc atcgggtaag cctaaatcca
     6781 gtaccaccag gctgtaatga ccggcttcaa ggctttgttc cgccatccgc gcggtgctca
     6841 cgccatcgca cgcgtagcct tcggtttgcg ccgccagaat caatccctgc aataacagcg
     6901 tatcgtcttc aacaatcaga attttcattc actcactctc ctgcaagttt gcagaatatc
     6961 atccgcagcc tggtaatact tcgtctcaac gccagtcagg cccaataagg ttgagaataa
     7021 attgtcttgt gaatagtgtt gcgtttgcgc ctgtttttgc aggcagttct ggtcaacctg
     7081 ataccgtttt tgataatcct ccgatagcca cagcagcatc ggcacctgtt tttggctatc
     7141 cggggcgatg gcataaggca gaccgtgcag atagatgcca ttttcaccta acgattcacc
     7201 gtggtcagaa agataaacca ggctggtggt aaatttatcc tgatgttctt tcagcagatt
     7261 aatcgcttta tcaacaatat agtcgacgta aaccagcgtg ttgtcgtaag tgttcaccag
     7321 ttgctcttgg gtacaggtct ggatctcgtt agtgtcgcag gttggggtaa atttcctgaa
     7381 ctgcggcgga tagcggttgt aataggtcgg gccgtggctg ccgatggtgt gtaagacaat
     7441 cacgccatca ccctgcaggt tattgatgta atcttccagc ccgtggaaca gcacttcatc
     7501 atagcattcg ccgttgatgc actgaccagg caggttcagc gcggtgacgt tctggtgagg
     7561 tacgcgatcg caaacgcctt tacagccgcc atcgttgtca ttccacagca cgttgatgcc
     7621 cgctcgctga atgatatcca gcacgccttc ctggtgctgt gccagctctt ctttgtagtg
     7681 ctcacgcggc atatccgaga acatgcatgg tactgaaact gccgttgccg tgccgcaaga
     7741 ggcggtatta gggaaataga ccacgttatc ttttgccaga cgaggattgg tttcgcgtgg
     7801 ataaccattc agcgagaaat tctctgcgcg cgacgtttct ccgacgatca aaatggtcag
     7861 gtttttacgt ttcccatttt gcattaatgg gttgcggtga gcatcttcac caatgcgtat
     7921 cagtggcagg ttggctgacc gctggtgaga gtaccacgac cagctagcaa caatgctgtt
     7981 ggacgggctt aaggatttca ccagttcttt gttgttgcga aacagcgagg catagtcttt
     8041 gtagaacagt gcggcaacca gcagaatcag cagtacagaa atcaggatgt tggccccgcg
     8101 gaaaagaacg ctgcgcaggc gtgaggtggt gggtttgatt ttgatccagc aggcaataat
     8161 cgcggctaat acgccactta accccagcgt taataacatt cgtggcgtca tcagggcata
     8221 actttccgcc ggcgtagtat caatgatatt ggtgatcatt gaacggtcga tgacgatgcc
     8281 ataggtcatg atgaaatatt gcgcagccgc accgaccaga ataaacaggc aggcgagtgg
     8341 acgattaagc cataagaaag agcctagtgt caggacaata ttaatcacgc tgaatgcgac
     8401 gaccggcatc gacaagaaaa ccagcacgtt atgcagtgaa tccagcggta acacctgtag
     8461 cacctgttta aaaaaggcga tattcaggca gactgagata taaaaagtag caagtaataa
     8521 ccaggagaat aaattcaaag agggtctttt tagtaggcgc ttcaacatca cggtgtttcc
     8581 atcgaacaaa gtgcgcatat gctcgcaaag caaaattaag ccaaccttaa gttcttaagg
     8641 ttggctttta tgtttgctgg attaagggga attaatcttt gcttattggt gcatctaagg
     8701 gatacgggtt tttatgcagc cggttgtaat tcagcgcata catggcggtg attaccatca
     8761 gagtgacaaa tgaccacata acctctttcg cgccggaccc tactacggcc cagatgcagt
     8821 agaggaaggc aatggtagta actgccaggt atgccgggcg cgctttacca aagtgaccgt
     8881 ggccgagcag cagtaacgcc gcacaggtgt aaagatatgg caccagtgta aagatgaccg
     8941 acacggaaga aaccagaccg aattctttgg tcgcgtttgg cgaaatgcta ctgagctgga
     9001 agatggtcat caaaataccg acgataatca gccccgccac tggcgtgccc gctttattta
     9061 cacgggcaaa aatcggtggg aacagtccgt catcggcagc ggctttcgcc gtttgacctg
     9121 ccagcaacgt ccagccgccc agtgaaccta agcaacccgc agctgcgcag aaggaaacaa
     9181 tggccccggc ggtgtcaccc aacgccatcc gtgcggcatc accgaatggc gaagcagaaa
     9241 cgcgcagtgc ggcattaggg atcatcccca taatcgcggt ggtagaaagt acatagcaaa
     9301 cggcagcaat caataccccg ccaatggtgg cgatagggac gttgcgtttc gggtttttca
     9361 ccacacctgc ggcaacggag gcactttcca caccgatgaa cgaccacagc gtaacgttaa
     9421 gggtactttg aatcgcaccg aaggtgccca ggccgctgac gttccatgcc gccatatagg
     9481 tttcaccacg gaaccagaac cagccaaata cggcaatccc gacgatgggg atcagcgcca
     9541 gcacggtagc aacggcctgt acgcgggtga tcattttcgg accgacaatg ttcagcagga
     9601 cgaagatcca cagcaccacg acgcaggtga tggttaatac caatggatct ttcagaatcg
     9661 ggaagaagta acttaaatat cctacgccaa tgaccaccat ggcgatattg ccgatccagc
     9721 aggccagcca gtagaggacg ttggtttgat aaccgagaaa cggcccaaag cagcggcggg
     9781 cgtaagcgta agaaccacca ggacttgggt cgaggaacga cattttggcg tataccatcg
     9841 agagccccag cgcaccgata atcgtcacca accatccata aatggcaatc ccgccagtgg
     9901 aggccaggtt tgcaggtaac agaaaaacac ctgaccccat aatattcccc gacaccatca
     9961 gggtgacggg gattaagccc actttgtgag catcagcatc cgaagacata attaaactcc
    10021 tgcgaaggcg agcttcgtga caataaatta cgtcatatca tacgcctgca ttcgatagtt
    10081 gaattattat cgaccgggtt atcgctgatg ccgttattaa tatagctctt cgttaaggtt
    10141 aaaaatcagg cgacagtgcg ttctctgtgc tgactaacat aatgcagagg cgtcataccg
    10201 tagaagtctt taaagacaga aataaagtac gacgtactgt tgtagccgca ggactgtgat
    10261 acctgagaga tatttttacc gtccatcatt aattcattta cggcatagcg catgcggcag
    10321 gtggtgatta tttggctata actggtgttt tcgcttttca gctttttctt taacagactt
    10381 gggctaagac ataaacaact ggctaccata ctaagattcc agtctttgtg gatatcgctt
    10441 tcaataattt gataaacgct gtcacttaca caattacgta acatatacat cattagtgaa
    10501 aggaattttt tactgtcgag aaaacgggat agcacagtga ataataatgc acgtgttcgt
    10561 tccttttctg actcagtttc agcgggcatc atgctatgtt gcgcggcaac gcgaaagacg
    10621 tctggcgtca ggcatggaag ggtgaggatg ggcgtagcgc tacgttgcca tactggtatt
    10681 tgcgagagat ctttattcag aaaccggagg taatctttga tgatgtcgtg actaatatga
    10741 gcaaccaaag tgttattaag agaggagacg tcgataatat tattttcaca atttaataac
    10801 gccatatggt tagctttaag gctaataggt tctttcccat tcacccttat ccagacatct
    10861 ttttcagtca ataaaacaat acaaggttgg tcgctgcaaa tcctcataca tgtactcctg
    10921 agtgcgaata aaagaccagt tctcgctaaa gcgaagcgat accgggaaat ggttatataa
    10981 ttgtatgatg aatataaaca tgcataaaaa taacgagttt tactaatatt acaaattttt
    11041 atagtaattt ctcatgtaca tattttttta caggctaaga aaaggataaa taacaggcaa
    11101 aaaaaagcgc ggggtgggac cgcgcaaaaa gcatgacatt ttttgtttgc agcaattttt
    11161 atgaaaacgt tgccatactt aaaaggcctc aagccaggcg gcgtaatgtt atttaacaat
    11221 tacgccttca gcggaatagt ggttacgctt tcacacacat aacgtggtaa ataccgtcaa
    11281 taatttctgt cccttctgtt tcgtgttcaa atccagggaa atgatgatcc caggattgca
    11341 gcgagcgtaa ataacttact tgcggactgt ttttatcgcc gaagttttca ccagacagca
    11401 gcatcgggat tcctggcgga tacgggataa ctgagtttgc cgcgatacgt cctggcagat
    11461 tttcaatgga taccagttcg acattgttgt cgacaatcgc gttgtacgct tcacgcgggg
    11521 tgatttccgc caccggtagg ccggaatagg cttcattcaa ccgtgcgcca gggttgtttt
    11581 ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat ccccatgttc gcgtaagtgt
    11641 caggatattg ttcaacaagt tccggcatca cctgcgccag cggtgtgttg gcgtcatagt
    11701 ggcgtttgaa ggagcaaagg gtgttaacca gagttcccca tttcccacgg gtcacgccca
    11761 tagagaacag gaacataatc tggaagtcag tggtacgggt aggcacaatg ccgtggcgac
    11821 caagccaggc tgtgaccagc gccgccggaa caccggtttc ttccagttca ccatcttcac
    11881 ccattcccgg agcaaggatg ctgactttaa tcgggtcgag catactccag ttatccggaa
    11941 tatctttgaa gccgtgccag ctttcgcccg gatgcatgac ccagcagtcc tgaacggtgg
    12001 tcagcagttt ggttggtgcg tcagcaaagt cataggtttt gccggtttgt gggtcggtga
    12061 cgacttcttt gttccacggt ttgaagaacc agctaccgtc agcggtgaac tctttatata
    12121 gccgcgccat cgcctgacgg aaatcaaccg cttcgtcgat cacttcctgg gtcagtgaca
    12181 ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt ggatgcgcag atggcataca
    12241 gcggggaggt ggtggcatgc atcatgtagg cctggttgaa gcgggagaag ttaatcgccc
    12301 cacgaccttc acgtacatga atataagagg cctgtgacag cgcattcagc agtttgtggg
    12361 tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc aggttcgccg cgcatggcat
    12421 agtgatcggc atagatcggg ttgaaacgtg catagccgta ccaggcttcg tcaaagtgca
    12481 gacgatcgga ggttttttcc agcagatcct gcgcttcttt agcgttataa cacacgccgt
    12541 cataggtaca gttggtcacc acgcagtaag acggtttttg cccggctttg tctttagtca
    12601 gcgggctttc attgattttc ttctgcaagg tttcaggttg catttcctgc ggatagattg
    12661 gcccgataat gccgtagcgg ttgcggcttg gcaccatgta gaccggtttc gcacctgtca
    12721 gcatcaagcc ttgttcgatg gatttatggc agttacggtc aacgaccacg acatcgttat
    12781 cggtcatgca agcctgcatg atggtgcggt tagagccgga agtaccgacg actaccgacc
    12841 aggagcgatc ggcaccaaat acgcgtgcgg catatttttc gctttcgcca aatgcgccag
    12901 tatggtcaag caaagaaccg agggaagtgc gttcgatgcc catgtcggtg cggaacagat
    12961 tttcaccata gtagtcatgg tagaaacgtc cggcgggtgt tttggtaaaa ccaacgccgc
    13021 cctggtggcc tggcgctgcc caggaatatt catggatgtc gctatatttc atcagcgcgc
    13081 tgaacagtgg cggcaatagc tgctggcggt agcgggtcat cgcggcaacg gcgcgtccgg
    13141 cgataaagtc ggcggtatct tccagaatcc aggcgaattc atcgacaagc tccagcaggt
    13201 cgcgatccat tgcggcgagg gctttttccc gatcgcccaa caggaagacc ggcacgtttt
    13261 gttggcgctc atgaagctta ccgatcaatt gtctgacgtt ttgatgttcg tccggatgtt
    13321 ccatttgata gctgaacatc aggcagtcaa tggcttcgtt tgaagagaga atggcaaaac
    13381 catcatcaaa ggaggtggat ttaatcacgg taacattttg ctggcttaaa gcatctgcca
    13441 gacgctcaac ggcgttaccg acccaggtgt cttgatggag aaactcgctt tcaacaatta
    13501 atactttcat cattgcttac ccggttatga aggaatcttc gttgcgggca agtatcttcc
    13561 ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt ttgaaatatt tatttttata
    13621 aacaatatgt tatgtgatta ttttaaatta ttttcactgc ttattatcga cggctaaact
    13681 atttttttgg ctgatactga tatcgtcttt agccgggaaa cgtctggcgg cgctgttggc
    13741 taagtttgcg gtattgttgc ggcgacatgc cgacatattt gccgaacgtg ctgtaaaaac
    13801 ggctactcga acgaaagcct gccgtcaggg caatatcgag aatactttta tcggtatcgc
    13861 tcagtaatgc gcgaacgtgg ttgatgcgca tcgcggttat gtactgtttc atcgtcaatt
    13921 gcatgacccg ctggaatatc cccattgcat agttggcgtt aagtttgacg tgctcagcca
    13981 catcgttgat ggtcagcgcc tgatcatagt tttcggcaat aaagcccagc atctggctga
    14041 cataaaattg cgcatggcgc gagacactgt ttttgtgtgt gcgcgaggtt ttattgacca
    14101 gaatcggttc ccagccagag aggctaaatc gcttgagcat caggccaatt tcatcaatgg
    14161 cgagctggcg aatttgctcg ttcggactgt ttaattcctg ctgccagcgg cgcacttcaa
    14221 acgggctaag ttgctgtgtc gccagtgatt tgatcaccat gccgtgagta acgtggttaa
    14281 tcaggtcttt atccagcggc caggagagaa acagatgcat cggcagatta agaatcgcca
    14341 tgctctgaca ggttccggta tctgttagtt ggtgcggtgt acaggcccag aacagcgtga
    14401 tatgaccctg attgatattc actttttcat tgttgatcag gtattccaca tcgccatcga
    14461 aaggcacatt cacttcgacc tgaccatgcc agtggctggt gggcatgata tgcggtgcgc
    14521 gaaactcaat ttccatccgc tggtattctg aatacagcga cagtgggcta cgggtctgct
    14581 tttcgtcgct gctgcacata aacgtatctg tattcatgga tgactctctt tcctggaata
    14641 tcagaattat ggcaggagtg agggaggatg actgcgaata ggcgcacggt tttattcctc
    14701 tccctggagg tgcgaggaga gcggatgaga agattggggt cgattattca gcttttcagg
    14761 taatttattc ccataaactc agatctattg atgtgccact taggatatga gtttatggga
    14821 atgctcaacc tggaagccgg aggttttctg cagattcgcc tgccatgatg aagtcattca
    14881 agcaagccag gagatctgca tgatgtctgc acctaaaatt acatttatcg gcgctggttc
    14941 gacgattttc gttaaaaata ttcttggtga tgtgttccat cgtgaggcgc tgaaaacggc
    15001 gcatattgcc ctgatggata tcgatcccac ccgcctggaa gagtcacaca ttgtggtgcg
    15061 caagttgatg gattcagcag gggccagcgg caaaatcacc tgccacaccc aacagaaaga
    15121 agccttacag gatgctgatt ttgtggtggt ggcatttcag attggcggtt atgaaccttg
    15181 cacggtgact gatttcgagg tctgtaagcg gcatggtctg gaacaaacca ttgccgatac
    15241 gctggggccg ggcggcatta tgcgtgcgct acgtaccatt ccgcacctgt ggcagatttg
    15301 cgaggacatg acggaagtct gccccgatgc caccatgctc aactacgtta acccaatggc
    15361 gatgaatacc tgggcgatgt atgcacgcta tccgcatatc aagcaggtag ggctgtgcca
    15421 ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc aatatcgatc cggctacgct
    15481 gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac ctggaactgg agcgtaaaac
    15541 cgcagacggt agctacgtga atctctatcc tgaattgctg gcggcttatg aagcagggca
    15601 ggcaccgaag ccaaatattc atggtaatac tcgctgccag aatattgtgc gttacgaaat
    15661 gttcaaaaag ctgggctact tcgtcacaga atcgtcagaa cattttgctg agtacacacc
    15721 gtggtttatt aagccaggtc gtgaggattt gattgagcgt tataaagtac cgctggatga
    15781 gtacccgaaa cgctgcgtcg agcagctggc gaactggcat aaagagctgg aggattataa
    15841 aaacgcctcc cggattgata ttaaaccgtc acgggaatat gccagcacaa tcatgaacgc
    15901 tatctggact ggcgagccga gtgtgattta tggcaatgtc cgtaacgatg gtttgattga
    15961 taacctgcca caaggatgtt gcgtggaagt ggcctgtctg gttgatgcta atggcattca
    16021 gccgaccaaa gccggtacgc taccttcgca tctggccgcc ctgatgcaaa ccaacatcaa
    16081 cgtacagacg ctgctgacag aagctattct tacggaaaat cgcgaccgtg tttaccacgc
    16141 cgcgatgatg gacccgcata ctgccgccgt gctgggcatt gacgaaatat atgctcttgt
    16201 tgacgacctg attgccgccc acggcgactg gctgccaggc tggttgcacc gttaaggggt
    16261 aatcgcttcc ggcgctctgg tgattaagcc aaagcgccgt agctggcggc aatgaaagcg
    16321 agcgaggaat atcaccgtcg ttaatgcgcc aagcagcgcg cagaacatat cggattgcgt
    16381 gtcccactgg tcgccctggg tgccgagaaa atcatcagct ccctggccca ttgccagcgc
    16441 tgccaaccac tcgattaatt catacatcgc gcttatcgcc agcgccacgc agcagaccag
    16501 aaacgccacc atcttacgtc cgcgaacgta catcccgcgt acaaggatct ctcgtgccac
    16561 cagtgcaggc accagcccct ggaaaaaatg tcccagtttg tcatacggat tacggctcaa
    16621 ccccagccat tcctgcacct caaaaccaac ggggactttc gcgtaggtgt attgcccgcc
    16681 gaccatcagg atgatggcgt gaaagaaaat gagcgtataa agcagcggcg ttaacggata
    16741 acgtttagcg gtggcaagca gtagcggcac gacaataatc accggtgtca cttccatcag
    16801 ccaggtgagt ttgtcatggg ccgaaatgcc ggtataaatc aggattaacg ttagcgccag
    16861 tgcgccagtg ttaagaatta acggcttgag tgtgcgggtc atggtgagtt cgcaagtcag
    16921 ggaaaatact gactattcac cagcaagcgt gaaattacaa tcgcaaaaga ggtggcccag
    16981 ggggatcacc tggcagcatg ctgccaggcg ctgggccgaa gaggttactt agtgcagttc
    17041 gcgcactgtt tgttgacgat ctgctggaag aagtcgttac ctttgtcatc gaccaggata
    17101 aacgccggga aatcttctac ttcgattttc cagatagctt ccatacccag ctccggataa
    17161 gcgacgcatt ccagatgctt gatgctctgc tgcgccagta ctgccgccgg accgccgata
    17221 ctaccgaggt agaagccgcc gtgtttatgg cacgcgtcgg taacctgctg actgcggtta
    17281 cctttcgcca gcatgatcat gctgccgccg tgggattgca gcagatccac gtaggagtcc
    17341 atacgacctg cggtggttgg gccaagtgaa cctgatggat aaccggcagg cgttttcgcc
    17401 ggacccgcgt agtagatcgg gtgatctttg atgtactgcg gcagttcttt accggcgtca
    17461 atcagctctt tcagcttggc gtgtgcaata tcgcgaccca cgataatggt gccggtgagc
    17521 gacaagcggg tggataccgg gtattgcgaa agctgggcaa ggatctcttt catcgggcgg
    17581 ttaaggtcga ctttcactgc ttcgccttca cctgcctgac gcagttcttg tggaatgtac
    17641 tggcctgggt tgtgttccag tttttcgatc cagatacctt cgcggttgat tttcgcttta
    17701 atgttacggt cagcggagca ggagactccc atgcctaccg ggcaggatgc gccgtgacgt
    17761 ggcagacgga taacgcggat gtcgtgcgcg aagtatttac cgccaaactg cgcgccaaga
    17821 ccgagtttct gtgcctcttc cagcagttcc tgttccagct ggatatcgcg gaacgcctga
    17881 ccatgttcgt tcccttccgt cggcagttca tcgtaatagt gagcgcttgc taacttgacg
    17941 gttttcaggt tggtttccgc agacgtaccg ccaatcacaa acgcgatatg gtacggcggg
    18001 caggctgcag taccgagggt acgcattttc tcgacgagga agtttttcag tttgccgggc
    18061 gtcagcaggg ctttggtttc ctggtagaga tacgttttgt tggcagagcc gccgccttta
    18121 gcgacacaga ggaatttgta ctcatcgcca tctaccgcgt acaggtcgat ttgcgcaggc
    18181 aggttcgtgc cggtgttgac ctctttatac atgtccagcg gcgcgttctg tgaatagcgc
    18241 aggttatctt cgatataggt gttatagacg cctttcgaca gtgcttcttc atcaccgcca
    18301 ccggtccaca cgcgctgacc ttttttaccg acgatgatcg cggtgccggt atcctggcag
    18361 gtcggcagca cgcctttggc ggcgatttcg gagtttctta agaattgcag cgccacgtac
    18421 ttgtcgtttt cgctggcttc tggatcgtga agaatagccg caacctgttt ctggtgtgcc
    18481 gggcggagca tgaaagaagc atcgtgaaag gcttgctgcg ccagcagggt cagggcttct
    18541 ggttccactt tcaggatggt ttcgccgtcg aagtcggcaa cgctaacgta atcggaagtg
    18601 agtagataat attcggtatt gtctttcccc atcgggaaag gtgcctggta gataaagggt
    18661 ttgtttgaca tagcttccag cctgtaactc tgtatttgtt attcgaaaat ggcgtgccgc
    18721 gtagttacgg gcggcacgca aagtgcattt ataagaaccc gtacatcgcg gcgaagatcc
    18781 agccgaagac gcacgatacg ctcacaccaa tcaaccccgg cagaataaag ctgtggttga
    18841 tgacgaagcg accgatgtgg gtggtgccgg aacggtcaaa ctgaatcgct gccagatcgc
    18901 tcggataagt cggcaggatg taataaccgt agcaagccgg tgctgaagcc acgatgtatg
    18961 ccggatcaac gccgatcgcc agcgcgaccg gaacaatcgc cgccagcgcc gcagcctgag
    19021 agtttacaaa cttggaaacc agcagcagaa caatggcata ggcccacgga tactctttca
    19081 ccatttcacc cagtacgccc tgaatttcag acatatgcgc accgaacatg gtttcagcca
    19141 tccatgcgat accgtacacc gccacgatgg cgatcatacc ggaacggaag acttcgtttt
    19201 ttgagataga cgcgggattg gttttggtca ggataataat cagcgccccg gtcagcagca
    19261 taaacatctg aataaccagt accatcgaca gcggtttgcc gccgaaggat ggacgcaggt
    19321 ccgaatcagc accaagaagg gcgactacag cgattgcccc gaggaagatc cacattgcca
    19381 gccagttgct tttcggcagt tttttatcca gcagcgtcgc ggtatcaccg taaacatact
    19441 cacggttttc cggtacggag atgaatttct ggaactcttc gtctttatcc agatctttac
    19501 cgcggaacca gctgaagata ccgatcgcca ggataccgat taacgtcgat ggaatggtga
    19561 ttgccagcag gtcgaggaac tcaagatggc gaccatcaaa ggtgacatta cccagcatcg
    19621 caaccagaga tacgaccgca accgacaccg gactggcgat aatccccatc tgtgcaccga
    19681 tagaacttgc cgccatcgga cgttccggac ggatgttgtt cttaatagca acgtcgtaga
    19741 tgatcggcag aatggtgtaa accacatgac ccgtaccgca aagaatggtc agcgtacagg
    19801 tcacaaacgg cgcgacaatt gagacatatt tcgggttgcg acgcagcagc ttctcggcaa
    19861 tttgcagcat gacatcaaga ccgcccgaag cttgcaaggt cgccgatgcc gccaccaccg
    19921 caatgataac cagcatgaca tcaaccggtg gtttacctgg ctgaaggtgg aagacgaaga
    19981 ccagaatgac cagaccgata ccgcctaata aacccagcgc gataccaccc tttctggcac
    20041 cataaaacag acatatcagt attatgataa gttggatagt aaataacatg tgtgaaccct
    20101 cgcgataatc ctatttaaat ttttgctgaa tagatcacag tcacgttctg ttttgtatga
    20161 actgttttca gagcctgact aaatatccgt ttggtcgtta ccggcttgag caaatacctc
    20221 acagtgaata ttggctgatt aaagcagcgg caaatttatg cactgtgata agcggctttt
    20281 tcagagagaa agactctctg gcgatgggtt tatagtttgt acagcgcaga tgttaatgtt
    20341 ttgtttgctg ttatcttatt gatatttatc ggtctgtttt ttgttctcag ggcaggtatt
    20401 attaggttta tagattaatc tgatctaccc atttgtgggt aaaaatacac ataatgtggg
    20461 tgacataata gttaattaac ttttgttagc gttttgaaat taaaaacacc gttcatctga
    20521 agagatatta atttttagcg atgatggagg gataattata tttgatctgg cacaagtttt
    20581 actgatgaac gatgtaactt gtgccagggg tattttgcat tacggtaata attattactt
    20641 acagataacc agcgatccgt tattggcaat attgtttcag tagtgagtag tgttctgcct
    20701 gaatacggta acggtaaacc ggacgccccg tgacgccata atggatactg gtgaacaaga
    20761 tgtggcagtt gaccagccag ataaggtatt tacggcagga aacacgcgag atgttaacct
    20821 cgttagccag ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca
    20881 gcgtgcgtaa cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc tgctcgttgg
    20941 agctgctgcc gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca
    21001 gcgccatttt cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg
    21061 gtttgatcag gtaatccaca acaccgtaat gcagcgaatc tttgatggtt gctgcatcgg
    21121 cagcggagga gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta
    21181 aatcgagccc gttctctttt tgcatataga tatctagcaa tatcaggtcg ataggcgtat
    21241 cgctattgaa gataatctct ttggctttct ccagcgtcga agccgttcca cagcattgaa
    21301 agcccgggat ttgtgccacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga
    21361 taattaatac attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa
    21421 ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg
    21481 ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc
    21541 aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcgcagtg
    21601 cagccagccg tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggtcctaa
    21661 tgcctccagc gcgttttcta tcagatttcc caacgtggta atcagcgtcg cgacctgatc
    21721 ctcactaccg ctgtctggca gctggctttc actgtttaaa atcagcgtat ggcctaaatc
    21781 ggtcgcgcgg ttaatcttgc tgattaaaaa accagcgata accggagatt tgatcttacc
    21841 cagcagagag ccaatctctt cctgatagtt attggctgtt ttgagaatgt aatcttccaa
    21901 ctgcttataa ctcttcagat gcaataatcc gagaatcaca tgcaatttat tcataaattc
    21961 gtgggatcgt tcacgaagtg cgtcagcata gttgaccaga ccgtcgagtc gctgcatcag
    22021 tttacgtact tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact
    22081 gcgcaccgga acggtgttga tcagtaatag tcggtcttta atcgtaatct cttcgtcgcg
    22141 gcgtggggtt ccgtcgcgta acacttccga gacatctacc acttgtgacc acgagtggct
    22201 tagcgtcgac agtttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc
    22261 atcgttgatc agggtgacct cgccgcgatc gtccacggca acgacgcctt ctttgatgga
    22321 ctgcaacatg gcctggcgtt gctcaaacag tgtggagatt tcgtagggtt ccaggccgaa
    22381 aaggattttt ttcagtactt taaccagaat gcatgtgccg atcagtccga ccagcatgcc
    22441 aaatagtacc gaccagataa tgctccagcg actgtcattg atctgttggg tcacgcggct
    22501 taactcaagg ccgatcgcca ctacgccaat ttgtttatga ttttcatcgt agatgggggt
    22561 aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata gcgacatttt cttcgccatt
    22621 cagcgcttta aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg
    22681 cgagtagcga agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg
    22741 cacggcttcc gcgatggcct ggattccact ctcctgcgct tttttctgca agccctgacg
    22801 gatttccggc gagtcggcga gggtacgcgc tactgccagt gccttgttag ctagcccgtc
    22861 tcgcgtcata tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa
    22921 cagtaccgcg ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa
    22981 catacggtag ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt
    23041 atctctgatg aggcgggtaa ttcaaaggga gtaagaatga ttggctatat aggggaagag
    23101 actctggcaa cggaaactgc cagtgctgta tgaagattcc ggggctatgc ttatagtgaa
    23161 aaccatactg atgagagagg gaaggtcatg gatcaggcgc tactggacgg gggttatcgc
    23221 tgttataccg gcgaaaatat cgatgtctat tttaacactg cgatatgtca gcattctggc
    23281 aattgcgtac gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg
    23341 gatgaagtcg acgtcaccac tgtggtgaaa gtgattgata cgtgcccaag cggcgcgctg
    23401 aaataccgtc ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataaatttt
    23461 acattaatga cgaacagggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga
    23521 atttagcgat tatcgaacat accgatgtcg atgaaagcct gaaaggacaa gggatcggta
    23581 aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccac
    23641 tatgcccatt tgcgaaacac gaatttgata aaatacggga gtatgatgat attcgcagtt
    23701 gaaaagatga tttgtgtccg ccggaagtaa tacaaatctg gaatgatgtt gttaatatgt
    23761 tagatgaaaa taaccccgca tatgcagggt tattaatcta agaactaccg actattggca
    23821 ttgtaaagtt aattcactga atttcgcttt tttattttgc aatttgtaat ttgttttaca
    23881 cttttgagaa gcctcctcac cccattgtgc aataagtgta tcctcttcag gtaaccctga
    23941 catatacacc tgtccttgat cagcaacaat actgctgtta atgctatttt cagaatctaa
    24001 tgtcaccaca ctaccaaacg gtacgggctc accattcttc tgtatcaagg taattattgc
    24061 cttattaccg acattgactt taaatggagc ggaaactaaa gctcctcgag aagggatgac
    24121 agtaacatct gtatttagca gctcaacatt atccttaaca ctattaacat caagtgaaat
    24181 ttgatttcgg ttatagggat tcaaatatgg gaccacggca tatccacgcc agtccgtata
    24241 aatacctgac ccgttcaata cttttacgct ggcggcatca cgagcctgaa ccagagcact
    24301 ggcactgtcc agacttaatg gctgggataa ggttaatcca tgtggatgca acgtaatacc
    24361 accgctaatt ccgtatgtcc attgactgga ttctttttgc caggcatagc cgccgttcat
    24421 ttgtgcataa ggtccacgat agttaaggtt aatgttacct gactctcctc tgttttgatt
    24481 ctgccagcct tgcgccacgg catagttaaa ctcaccatcc tgtgaggagc cgtttaatcc
    24541 aacctgctgt tgcacctgac cattattcgt aatggtattc gtggttaaga acaggttatt
    24601 tccattcagc aatgagttga gcggtacaga cacagataaa aacagcgatt tatcagcctc
    24661 gtggtaatac gggcttttat tatacccgag cgatagcatt gccgagatat agcggaatgg
    24721 cagcgagtaa ctcgcagata agttatatcc atcagcggta tcccagtact gatctttact
    24781 tgctgagagg tagagttgtc cccaatcctg aagatcttga cttatcgttg ttgatatacg
    24841 cgaacgttga tgataactgt ttatattttt ctcatcaacg aatccactct gaatttgttg
    24901 taactcatca aaatcgtagt aaccgcgtgt ggcataacga tagccggtaa tattcaatgt
    24961 cgtattcgtc tcaccaaaac cacgcgaata cataaagcgg taagactgtc cttgctgttt
    25021 tccgaaagac ttataattct gcgaaaattg tgagcgagca tgagtgacat ccagggagaa
    25081 cgctccaaaa aaatctgagt atttacccag gccaagcaga actgctgcat agttgtctgc
    25141 tttgatacca ccaccataaa atgtatattc ataaggtaat ccgattgctg ctgaagactg
    25201 aacaaactga ggctcagaca cactattatt caaagtagaa cttgagcgat atttcccgaa
    25261 tgccaggtta tagcgtaagc gcccctgacg ttgcagaatc ggtaaaacgg ccgtcgcctg
    25321 agtaaaatgg cgaatagtgc catctgcctc acggatttcc acctccaggt caccggtgaa
    25381 tatttggtta aaatcgcgta attcaaacgg tccaggtggg acgttctttt gataaatgac
    25441 aactccgttt tgcttgacgg atacctgtgc ttgagaataa gcaatgccac ggacaacagg
    25501 actatattgg ctgagttctg gggcaatcat gccatcatca gatgaaaact gtgctccttc
    25561 aaaaggaaaa ctgtcaaata tcgcaccatc cgttgaggtt tgccctacag taaattgccc
    25621 tccctgcagg aaagaataat catgctgtag ataaacacta acgggttgcc agtggctctc
    25681 tttattatat gagtttgttg accagttacc gttatggcga aggcgccatc caccaaggtt
    25741 cagagaactg tccagagata aaaactgact acggctaccg tagccattat tattttgctt
    25801 aattccgtta aatagatagt taaccattag cgctggcata ccttcatccc agagatgggg
    25861 cggagtactt aaccaatcct gaggtttgag gtagctttgt ggtatattca atattagctt
    25921 ttggttgttc gcatcgaatt tctctgttgc gccctgaata atatatgcaa tattttgctg
    25981 gtaagccgat ggggaagata cggagagctt atttgtatca attccccaac gttccaactg
    26041 ttgaggcgtc agtctggcgt tcagcttgtt gtcctgcgcg aaaaaatcta tcttaatctg
    26101 acggtcaact aattccccat tcataactac atcaacagta tattcccctg gagctgcacc
    26161 gcttgcgttt gcaaaataat tgagatcgat atttttggca ttatcaccat cagataattg
    26221 caaaaaatcg gggttaaatg agaacctatg actattgtca ggtgagggag atacggcagc
    26281 gcaactgaat accggacata tgatggtgat ccttaacaat aataaggatt tttttaatga
    26341 acctttcaaa ttgtcccccg ataaagatga ttaaataaat gttaaagttt aatgttttga
    26401 atcggcgttt ctccgccgta atcatttatc gtagaataac taattgtttt agcatttttt
    26461 tccgggatga ttaatgtt
//
LOCUS       DAJOTB010000058        25918 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJOTB010000058 DAJOTB010000000
VERSION     DAJOTB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.058"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(101..748)
                     /gene="ychE"
                     /locus_tag="OHN28_002362"
     CDS             complement(101..748)
                     /gene="ychE"
                     /locus_tag="OHN28_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCQ2297755.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1225..3900
                     /gene="adhE"
                     /locus_tag="OHN28_002363"
                     /gene_synonym="adhC"
     CDS             1225..3900
                     /gene="adhE"
                     /locus_tag="OHN28_002363"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCQ2297756.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4025..5072
                     /locus_tag="OHN28_002364"
                     /pseudo
     CDS             4025..5072
                     /locus_tag="OHN28_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5358..5975)
                     /gene="tdk"
                     /locus_tag="OHN28_002365"
     CDS             complement(5358..5975)
                     /gene="tdk"
                     /locus_tag="OHN28_002365"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCQ2297757.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQAYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            6579..6992
                     /gene="hns"
                     /locus_tag="OHN28_002366"
     CDS             6579..6992
                     /gene="hns"
                     /locus_tag="OHN28_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCQ2297758.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7137..8045)
                     /gene="galU"
                     /locus_tag="OHN28_002367"
     CDS             complement(7137..8045)
                     /gene="galU"
                     /locus_tag="OHN28_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCQ2297759.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8247..9260)
                     /gene="rssB"
                     /locus_tag="OHN28_002368"
     CDS             complement(8247..9260)
                     /gene="rssB"
                     /locus_tag="OHN28_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCQ2297760.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9352..10257)
                     /gene="rssA"
                     /locus_tag="OHN28_002369"
     CDS             complement(9352..10257)
                     /gene="rssA"
                     /locus_tag="OHN28_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCQ2297761.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10370..10828
                     /locus_tag="OHN28_002370"
     CDS             10370..10828
                     /locus_tag="OHN28_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCQ2297762.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10878..11720
                     /gene="purU"
                     /locus_tag="OHN28_002371"
     CDS             10878..11720
                     /gene="purU"
                     /locus_tag="OHN28_002371"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCQ2297763.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11880..11964
                     /locus_tag="OHN28_002372"
     tRNA            11880..11964
                     /locus_tag="OHN28_002372"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11914..11916,aa:Tyr,seq:gta)
     gene            11998..12082
                     /locus_tag="OHN28_002373"
     tRNA            11998..12082
                     /locus_tag="OHN28_002373"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12032..12034,aa:Tyr,seq:gta)
     gene            complement(12099..13190)
                     /locus_tag="OHN28_002374"
     CDS             complement(12099..13190)
                     /locus_tag="OHN28_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HCQ2297764.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(13177..14301)
                     /locus_tag="OHN28_002375"
     CDS             complement(13177..14301)
                     /locus_tag="OHN28_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HCQ2297765.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(14294..14896)
                     /locus_tag="OHN28_002376"
     CDS             complement(14294..14896)
                     /locus_tag="OHN28_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCQ2297766.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(14893..16179)
                     /locus_tag="OHN28_002377"
     CDS             complement(14893..16179)
                     /locus_tag="OHN28_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HCQ2297767.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(16176..16982)
                     /locus_tag="OHN28_002378"
     CDS             complement(16176..16982)
                     /locus_tag="OHN28_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCQ2297768.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(16960..17976)
                     /locus_tag="OHN28_002379"
     CDS             complement(16960..17976)
                     /locus_tag="OHN28_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2297769.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(17973..18980)
                     /locus_tag="OHN28_002380"
     CDS             complement(17973..18980)
                     /locus_tag="OHN28_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCQ2297770.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(19108..19785)
                     /gene="narI"
                     /locus_tag="OHN28_002381"
     CDS             complement(19108..19785)
                     /gene="narI"
                     /locus_tag="OHN28_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2297771.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(19785..20495)
                     /gene="narJ"
                     /locus_tag="OHN28_002382"
     CDS             complement(19785..20495)
                     /gene="narJ"
                     /locus_tag="OHN28_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2297772.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMEPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(20492..22030)
                     /gene="narH"
                     /locus_tag="OHN28_002383"
     CDS             complement(20492..22030)
                     /gene="narH"
                     /locus_tag="OHN28_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2297773.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(22027..25770)
                     /locus_tag="OHN28_002384"
     CDS             complement(22027..25770)
                     /locus_tag="OHN28_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCQ2297774.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         6662 a         6888 c         6237 g         6131 t
ORIGIN      
        1 tttttaccta ttccttttgc ttttgataac tgcgcaggaa caaagtaaaa ataatgacga
       61 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
      121 aaatcccctt aataccagta acgataaatt caatccccaa cgccatcagc aatagcccca
      181 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac tcgtaccagc cacggtgcca
      241 tgcgaaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
      301 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
      361 ccgccatcaa cggcaacgcc aatggcacca caccaatgct ttcacgcacc gcggtttctg
      421 atttttcttg cttgttctgt ttatcctcac caagcttgcc gctgatcatc gacatcgcta
      481 ttgtcaccac caggatgcca ccggcgatac ggaatgaatc aattgatata ccaaaaagtt
      541 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
      601 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
      661 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
      721 cgggaaaatc aaaaaaggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
      781 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
      841 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
      901 atgtagccaa caaatcatac tacaatttgt taactgttag ctataatggc gaaaagccat
      961 gctgaaaggt gtcagctttg caaaaatttg atcttgatca cgtaatcagt acccagaagt
     1021 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
     1081 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt tccatcggtt
     1141 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
     1201 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
     1261 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
     1321 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
     1381 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
     1441 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
     1501 gacacttttg gtaccattac tatcgctgaa cccatcggta ttatttgcgg catcgttccg
     1561 accactaacc cgacttcaac tgctatcttc aaatcactga tcagcctgaa gacccgtaac
     1621 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
     1681 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
     1741 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
     1801 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggcgta
     1861 ggcgcgggca acactccagt tgttatcgat gaaactgctg atatcaaacg tgcagttgca
     1921 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
     1981 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggtggctat
     2041 ctgttgcagg gtaaagagct gaaagctgtt caggacgtta tcctgaaaaa cggtgcgctg
     2101 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
     2161 ccagaaaaca ccaagattct gatcggtgaa gtaaccgttg ttgatgaaag cgaaccgttc
     2221 gcacatgaaa aactgtcccc gactttggca atgtaccgtg ctaaggattt cgaagacgcg
     2281 gtagaaaaag cagaaaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
     2341 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacggct
     2401 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
     2461 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
     2521 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
     2581 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
     2641 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
     2701 aatggctatg ctgatcagat cacttccgta ttgaaagcag caggcgttga aactgaagtc
     2761 ttcttcgaag tagaagctga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
     2821 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgctgcg
     2881 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagaact ggcgctgcgc
     2941 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggtgtgaa agcgaaaatg
     3001 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
     3061 gacgacacta ctggtcagaa atatccgctg gcagactatg cactgacccc ggatatggcg
     3121 attgtcgacg ccaaccttgt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
     3181 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
     3241 ggtcaggctc tgcaggcact gaaactactg aaagaatatc tgccagcgtc ctaccacgaa
     3301 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcgactat cgcgggtatc
     3361 gcgtttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
     3421 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaac
     3481 gcgaatgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
     3541 cgtcgttatg ctgaaattgc tgaccacctg ggtctgagcg caccgggcga ccgtactgct
     3601 gctaagatcg agaaactgct ggcatggctg gaaacactga aagctgaact gggtattccg
     3661 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
     3721 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
     3781 gagctgaaac agattctgct ggatacttac tacggtcgtg attatgtaga aggcgaaact
     3841 gcagcgaaga aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
     3901 tcagtagcgc tgtctggcaa cataacggcc ccttctgggg taatgccagt cagttaagga
     3961 tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcactcgt ttccggattt
     4021 ttttatgctc attggacagg cccttgattt ggtatcccgt tacgattctc tgcgtaaccc
     4081 actgacttct ctgggggatt acctcgaccc cgaactcatc tctcgttgcc ttgccgaatc
     4141 aggtactgta acgctacgca agcgccgtct tcccctcgaa atgatggtct ggtgtattgt
     4201 tggcatggcg cttgagcgta aagaacctct tcaccagatt gtgaatcgcc tggatatcat
     4261 gctgccgggc aatcgcccct tcgttgcccc cagtgccgtt attcagaccc gccagcgtct
     4321 gggaattgag gctgtccgcc gcgtgttcac gaaaacagcg cagctctggc ataacaccac
     4381 gccgcatccg cactggtgcg gcctgaccct gctggccatc gatggtgtgt tctggcgcac
     4441 accggataca ccagagaacg atgcagcctt cccccgccag acacatgccg ggaacccggc
     4501 gctctacctg caggtcaaaa tggtctgcca gatggaactg accagccatc tgctgacggc
     4561 tgcagccttc ggcacgatga agaacagcga aaatgagctt gctgagcaac ttatagaaca
     4621 aaccggcgat aacaccctga cgttaatgga taaaggttat tactcactgg gactgttaaa
     4681 tacctggagc ctggcgggag aacaccgcca ctggatgatc cctctcagaa agggagcgca
     4741 atatgaagag atcagaaaac tgggtaaagg cgatcatctg gtgaagctga acaccagccc
     4801 gcaggcacga aaaaagtggc cgggactggg aaatgaggtg acagtccgcc tgctgaccgt
     4861 gacgcgcaaa ggaaaagtct gccatctgct gacgtcgatg acggacgcca tgcgcttccc
     4921 cgggacgtat accggagctg atgcgcgatc ttgcaagtat gggacaacta gtgaaattac
     4981 cgacaagaag agaaagggcc ttcccgagag tgataaagga gaggccctgg aaatactcca
     5041 cagccccgaa aaagagccag tcagttgctt aactgactgg cattacccct tctggggccg
     5101 ttttttattt tcccaaagca acttttccat aaaccggcag cattgtcctt catcatattt
     5161 gcgacgatgt ataacgccta cacacagcga tattgtactt tacaggttac aagtcaatgt
     5221 cggtgtataa gcgctctgtg aggcgtataa cggcgttaaa aaactgccga gaagggtgta
     5281 tagcccggaa gaagtgcgta aaacgaactg acagggataa aagcgccctg ctcaccctgt
     5341 cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt taatgagccg
     5401 acttctaacg cctctttata gtgtttacgg catacagaaa cgtagcgttc attaccacca
     5461 ataaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg cagcaccatg
     5521 cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc ggaccatgcc
     5581 agtaagtatt gactgccaat aaataattca cctcgaaaat cggtacgtag accataacaa
     5641 agtacgggta tatcgagttg atcgacaacc tccgataatt catatgcttg ttgtctggtt
     5701 aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg ttccgcacga
     5761 atctcatcaa ataatgatga attttggtta aataattttg caggtgatga caaacctata
     5821 cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata tacgacagtg
     5881 cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga cttacccgca
     5941 ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc aatgatatga
     6001 aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca gtatgcacag
     6061 aagtggtata tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca
     6121 actgcctcgc ttgttataag cgggtaaatg actgccggta actattcaca atctttaacc
     6181 tgttgcgcat gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccctg
     6241 ctcttattgc gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacacg
     6301 actaatacag aagactgaaa ggtcgtcagc ctacgattat ctccccataa aatgtgacat
     6361 gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg gtgcaaatag ggctatatgc
     6421 cgcgtctttt ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca
     6481 attttgaatt ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc
     6541 tcaacaaacc accccaatat aagtttgaga ttactacaat gagcgaagca cttaaaattc
     6601 tgaacaacat ccgtactctt cgtgcgcagg caagagaatg tacacttgaa acgctggaag
     6661 aaatgctgga aaaattagaa gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg
     6721 ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata tcgcgaaatg ctgatcgctg
     6781 acggtattga cccgaacgaa ctgctgaata gccttgctgc cgttaaatct ggcaccaaag
     6841 ctaagcgtgc tcagcgtccg gcaaaatata gctacgttga cgaaaacggc gaaactaaaa
     6901 cctggactgg ccagggccgt actccagctg taatcaaaaa agcaatggat gagcaaggta
     6961 aatccctcga cgatttcctg atcaagcaat aatcttttgt agattgcaca tgctaaaaat
     7021 cccgccatcg gcgggatttt ttattgtccg gtttaagaca atttaataag aataagctat
     7081 aaaaaaaacg gcgtcgattg ctcaacaccg tttcgtggaa aacaccgata cggatgttac
     7141 ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag ggtgttatga
     7201 cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca gtcatggctc
     7261 ttccctttca tatgataggc ttcaaccgtt tctttttcga tcagcatatc aattgcgtcg
     7321 gtgagctgaa tttcatcacc agctccagga ggggtttttg ccagcaacgg ccaaatatcc
     7381 gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc cgcttttggt
     7441 ttttcaacca cgccaaccat cggtacgctt tcacccggcg ctaattcaac gcctttgcaa
     7501 tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg gctatgaccc
     7561 gtttcatcaa agcggcggat catctctgcc aggttatcct gtgataaatc ggattcatat
     7621 tcatccagaa taacgtcagg caaaataaca gccacaggtt catcacccac taccgggtga
     7681 gcacacaata ccgcgtgtcc caggcctttc gccagcccct gacgaacttg cataatagtc
     7741 acgtgcggcg gacaaataga ctgcacttca tcaagcagtt gacgttttac acgtttttcc
     7801 agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt tttagatgag
     7861 tgagtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac gacgtattga
     7921 attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt cgccggcaac
     7981 atcctggttc ctaatcccgc tacggggata acggcttttt tgactttcgt attaatggca
     8041 gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat
     8101 cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt tagcataatt
     8161 aagacaatta cttatacgaa tagcgacgat agtaccacca gccgacatta gcaggtaatg
     8221 caaatttagc ccgtgttatc gtttgctcat tctgcagaca acatcaagcg cagccgacca
     8281 ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg attcaaataa
     8341 gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctgatg ttcgccggta
     8401 tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc gcgatgataa
     8461 tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa atggtttacc
     8521 tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag ctgttcctgt
     8581 aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt atgtccagct
     8641 cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc aatatcaagc
     8701 accaggccgg gtttgtccgc ggcaactaat tgacgataat taacccggca ttgggaaatc
     8761 acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcagc agggttatca
     8821 accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcggga attaaacatg
     8881 ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt aaccggtttc
     8941 agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat attttcagtg
     9001 gcagatatca ccagaactgg tgtctggtca cctctgttgc gtatatgttc cagcagttta
     9061 agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt gaaacctccc
     9121 agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa
     9181 aaccacgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag aatctgtttt
     9241 ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata gtggcatgct
     9301 attgccacct acgcctgtca gaattaactt aagtgtagat aaaaattctg gtcaaatgtt
     9361 ggtgcgtacc aacggcaaaa gttcgtccat tttcttttcc actgccagct gtccggccgc
     9421 aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat
     9481 cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct ccagcacctg
     9541 gatagaagtg gtcataatct ctgttgccgt tggcgctgtc accgcccgac gtgtcgttat
     9601 gctgcctaac ctttctttca gacgcgcatg ccacggcaga gaatcaccat tctcgctatt
     9661 ttcttcactg acattaaagg agagcaaatc ttgttgcatc aaatgagcat cgtgctgcag
     9721 gtcgaccgct atcacaatat cagcccccaa tgcacgcgtg agggaaatag gaattgggtt
     9781 aactacagcg ccatcaacca gccagtagcc gttatgcgca acgggtgcca ttagtcctgg
     9841 aatactgcat gatgcacgaa tagcaagatg gagatcgcct tcagtaaacc ataattcacg
     9901 cccagtactt aaattggtgg caacagccgc gaagcgacgg gaacaatttt cgatctctgt
     9961 ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta acccaccgcg
    10021 ctgccaggag agatccatca ggcgtaaaac atcccaataa ctgaaagagg tcacccaatc
    10081 ttccagcgca gataatcgat cgcatgcata ggcagcgccc accagcgaac caattgaaca
    10141 tcctgcaacg atatcaattt caatacccac tttttttagc gcattaatta cgccaatatg
    10201 cgaccaacct cttgccgcgc cagatcccag cgccagccct atttttatct ttctcattat
    10261 tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg gttaacatag
    10321 tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg tgtctcagct
    10381 ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg tgtctggtga
    10441 aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt ttgtgatgca
    10501 agacgcagat tatttaataa agacctggca tccatcttgt ggagcagcgg cgttacgtgc
    10561 cgaattgatt gccggatttg cgcatactga atggttggga ttgaccgtat ttgaacattg
    10621 ctggcaggac ggcggcgata tcggatttgt cagttttgtt gctcgattta ccgagggtgg
    10681 caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc aatggtacta
    10741 tattgacggt acacgtccgc agtttggccg caacgatccc tgcccttgtg gttcaggtaa
    10801 aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca aacactctca
    10861 acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact atttgtccgg
    10921 accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag ttaaatatcg
    10981 tgcagaacaa tgaatttgta gatcatcgta ccgggcgctt ttttatgcgc acggaactgg
    11041 aagggatttt taatgattcc accctgctgg cagatctcga tagcgcattg ccagaaggct
    11101 ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc actaaagaag
    11161 cacattgcct tggcgatttg ctgatgaaag ccaattacgg cggcctggat gtcgaaatcg
    11221 cggcagttat tggtaaccac gataatttac gttctctggt tgagcgtttt gatattccgt
    11281 ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag atggcggatg
    11341 ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg gtattaacgc
    11401 cggaatttgt ggctcgcttc ccgaataaga tcatcaatat tcaccattcc ttcctgccag
    11461 cgtttatcgg cgcacgtcct tatcaccagg cctatgagcg tggcgtgaag attattggcg
    11521 caaccgctca ctatgtgaat gacaatctgg acgaaggtcc aatcatcatg caggacgtta
    11581 ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga
    11641 aaaacgtctt aagtcgtgcg ctatacaaag tactggcgca gcgcgtcttt gtttacggta
    11701 atcggacgat tattctttaa tcgtcagcca aaataactgg ttagctttaa tccgttacgg
    11761 atgaaaatca cgcaaccagt tcatttttct caacgtaaca ctttacagcg gcgcgtcatt
    11821 tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca ttaccccgtg
    11881 gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag
    11941 gttcgaatcc ttcccccacc accatctcaa gcattaccta caatatgaat tacccctggt
    12001 ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt
    12061 tcgaatcctt cccccaccac catcactcga ttccattatc aaaccttcgg cttctccccc
    12121 attcgccgta aaaactgctg ctgtagcctc aacaaatccg ctaacgtcaa caactgataa
    12181 tccagacctt gctggctggc aatttgcgca ataatgcggc ttagcgccga ataatggcga
    12241 tgatgccagt gaggaaacag atgatgggta aggtgcagat taatcccacc cagccagtag
    12301 ccaagccatg caggttgcgg cgtccaatca aaggtggtag caaaggtatg cgccagtctg
    12361 cccacgggca ttttgccctc ttctggtggt aactgcgtat gtcccttcgc ccagtgcgtt
    12421 ccaataatca acgtcacaaa aatcaatgac gccagcagct ggctgagcag ataagtcagc
    12481 aggatggtca taaaactaaa atcagacaaa agccagggaa ttatcagaca cagcgcacaa
    12541 tgcgccagtt tcccggctaa gaaatatccc cagccagcaa agcctcgcag ggccagatgt
    12601 cgcgtgaccg gcgtaaaccc tgcgcgatcg agccagtcca ctacccagat ataccaggga
    12661 aaggtgagcg cggcgaccag tggccaatag cgatgttgct ggcgcataaa aggtcgccac
    12721 cgttgccacg gcgtctggcg aagaatgcca ttttctgctg tatctggatc gtaaaattca
    12781 atattggtaa aaccgtgatg aaagcgaacg tggcgcaccc gccagcagtc cggatctagc
    12841 cccataggaa aagcaaccca ccggttaagc cgggcattgg tctttttacc acgcaaaaac
    12901 gcatcatgcg aagcgtcatg caccacattg accgctaaca acatggcgca aaacatcatc
    12961 gcgagatacc acaacagata cagtccccat tgacgttgaa ataacgccag taagtaacag
    13021 gagaaacaag caataatcaa caccgccatt ttgacgaatg cccatccgtc ggcataccga
    13081 tgatcgcctg atttcgccag ataaactttc gtaccgcttt gcagttgttt caccagttgt
    13141 ggatcatcgg caggaaaagt gattaacctg tcgggattaa acaggttcat actgtttcct
    13201 tagcgattta cgtcctacgc cgattacaaa taacaggcaa tgggccagca gaatacccac
    13261 taatcccttc cagagcagag tactccatga acacatatag acaataaaat agagcagtga
    13321 caacgtcaca gagcaccaca aaatcatacc ccaatgctgc ccgtcgctca tgccccccag
    13381 cgcaacggag cctgccgcca gcagaacaaa taatacggct tgctcgctgg taataaactc
    13441 aatgccatag ccatattgtt gaatgtagcc aagcaccagc ataaacagca ggatcacccc
    13501 tgcccctacc gcccatggag aactctggta gcgaccttca atcgctttgc caaatgagca
    13561 aaagcccatt aagcgtaaaa atggcagatt actctcgcgc atcgggttac aggacaagcg
    13621 actatttttt acgccgtagc ctacgtcgta ttccggcccg gcactatacg aaccgaaaag
    13681 tttatcccac aacaaaaagg tgccaccgta attggtatcg gcatattgct tttgattcag
    13741 atgatggacg cgatgatgcg aaggcgtgat cagcactttt tccagccagc ccagtttcgg
    13801 cgttagtgcg ttatggttaa aaaattgcca actatagtga ataatagaaa ccgccaaaaa
    13861 gacctgtaac ggcacaccaa gcatcgccag cagcataaaa aacgggattg aggttaacga
    13921 ggagtaccac gagttccgca cgcctaatga caggttgtag tgttccccct gatgatgcac
    13981 cacatgtacc gcccacagcg ggcgcatttc gtgatgccag cgatgtagcc agtaaaagcc
    14041 gaaatcccac gccaggatag taaacaacca cagcaggatc accggcacat tatcaaacag
    14101 tccaaggctg aagtgattga ccaccaggcc atagcagagc acttccaggc agcgaaataa
    14161 ccacagcatc atatgaccgg agttgatatt aaaaataacg tccagccagt ccactttttg
    14221 ccgcccggtg aattgcaaca cgcccgcttc ggctaacacc aacagcacca tcgatatgat
    14281 ggggaataat aaatcactca tattgtgtct tccttttctc gcatgttgcg catatccaac
    14341 cgcaaatcga cgccagcaac gcgccgccta ctaaatcgat aaataaatgt ctgcgtaatt
    14401 gcagaatcga aaaggcgatt gctatcgccc agaccacgct cattagcgtc agccaacggt
    14461 tccccttttt agcaatcgcc cataccgcca acagcgttag cgcggcgtgt aaggagggaa
    14521 aacagttctg gcttgagtca atggcgatca gcctgttgag tgcgcccgcg gatatcccca
    14581 tctgatcaaa cgagggataa tgcatggttg tgggccagag aagataaaat acgccagcca
    14641 ccagtgcgga aatttgcatt gaacgggcca gccagcgcag gtggcaaagt ggcgtggata
    14701 aataacctaa cggcacaatg aaaaaaaacg agagatacag ccacacagcg gagggggaaa
    14761 acgcaatcaa attatcaatt attgacggtg ttaattgata tccctcacct tgcaggtagt
    14821 cgctaaagtt gtagataacc ccaacagttc cccagcccag cagcatctgt ttcaggcgta
    14881 gcagtgaatt gctcattccc gcccccactg acgcacaatg cgccgccgct tggctgtaaa
    14941 agagactggc acagtttgca gttccagcga ccaaattaat tgcccgacag cgatatcctg
    15001 gcgtataaac aaggcattca acgcctcccg gcaatgctcc agcatccaca gctcgcaatc
    15061 ggcgattaac gtcaattgtg ctgacccggt ttgtagcagg cgataatcga ctgttagcgg
    15121 cagtgtcatt gccagcacac gactacaggc atcggcaaaa acggtctgaa tatcgccgga
    15181 tcttgttggt agttgcagtt ggtcatcctg ccgcccttcg atacgcgcaa tcgccattgt
    15241 tgcactgcca caggggcatg gctgttcgct tgccaccagc acatcatcca gtcgataacg
    15301 cactatcggc tgtgtggtgc gggtaaagtc tgtgacgatc ggcacgaaac ggtgctcatc
    15361 cagccattgc ggttcaatgt gaacaaactc ttcgttgaga tgtaacgttc cacaccggca
    15421 ggtggaagcg agaaagcctt ctgtcgcctg ataaatttcc cccacattgt ggaacacgtt
    15481 gcgcagtaat tcccgatcct gcggctccag aacttccgcc accgaaatca cttttttgac
    15541 ctttagcgtc aattcgccgg ccatcacctc cagcgccagt gcgcgtaata cctgagctgg
    15601 cgcgacaata atcgtgggcg ctcgctgttg gagaagtttg agttgcgcct gaaatggcgc
    15661 tagcagatcg taaaaatcga gacttaacca gcgattattc acgctttgat acaggttgtt
    15721 gtccgcgcgc agaaacagcg ccacccgctc tttggcaaat aaatcatcgg gcaacacttt
    15781 tgccagcaca ccggcggccc acatctgttg ctcgtgcggg ctaacaacaa aaagtccacg
    15841 acgcccggat gtacctgatg aaagcccaac gctgaactta ccaacgcagg gtttgaaatc
    15901 acgactctgc tcactgcgca ttgcacaatc gagaagttcg tcgcgcttta aacctgcggt
    15961 attcatctca tcgaagtgcg tcatcattaa cgctttatcc atcattggcc actgattaaa
    16021 gggcaacgtc agataacgct gaaaccacgg gctatgagat aagacatttt gccggaactg
    16081 atgcagcctt tttgcctgcc agttttccag tgcctcgcga tcggtgaaat gcagcctgcg
    16141 ggtacggaag tagcgccaga tcaacatcaa cgggatcata aatccccctc atggcacaaa
    16201 cgaatctcca ccacaccgtt ttgccaaagc tgatggagtt gcccaagcgt gtggtagtag
    16261 gcgtggctgt cggacataac cagatgcgcc agtggtgaag gccctcgcag ctcccggtag
    16321 ttagctggcg cccatgccgc atcgccagcc agtaaagtcc agccgttgtc ggttaacaca
    16381 aacgcgccaa tatgtccggc agcatgtcct ggaagcggca cgagaataat ttcgccattg
    16441 ctttctggca gcaaatagcc gcactcaaac ggtgccagtt gcgcgggcaa attcaccgat
    16501 gcgaaacttt caataaagcg cagtgaggag tcaaaattct ccgggattag ccctggcaca
    16561 aaggcctggc gcagcgcagt gacaccacgc agagttctta cttgccgcca gccttcacca
    16621 gaacagatgc aggaaacatc agggaaatca cgcaggccag caatatgatc agcgtgaaaa
    16681 tgcgacagaa taatcgcatc aacatctttc gctgcgaggc cggtggcaga aagttgtcgc
    16741 accagcgcct gttgcggatc gaaataaacc ggcgtgacct ggcgataaag acgaaacaca
    16801 ccagattgcg tgtagtgttc gaaccacgat gaatagcctg tgtcccacag ccaccgtttg
    16861 tcgcccacct ccagcagcca ggcgcgggcg gggaatttgc acactttcag ccctgcccct
    16921 ttcagcgcaa tacagctaat atgggtacac cagcccgttt caaatgcggt tatttttgcc
    16981 atgttccctc agccattgtc ccgtgattgc aataccttgt tccatcgaaa agcgtgggcg
    17041 atatcccagc tctgtaatgg ccttttgcgc actcagcgtc atatcgaaac agagtgttcc
    17101 ggcgctgtat cgtgtgataa caggttcctg attaacgcac ttcccataca gttccaggcc
    17161 acgcgcgatc agagaaagca gcgaccaggg aacagcagca atgcggtaac tcaagcccag
    17221 ttgctggtgc agcaaggcgt caagcatcac cgatagctgc tgcggctggt gattgctgat
    17281 gttgtaaata ctgcccgagc gtaagccttc aacgttcgtt gccagccgca tggcatacac
    17341 cacgttttgc acgaacgtga gatccaataa cgcgtcgccc ccgcgcggca aacgtaagca
    17401 accgccgccc tgttgcaatt gctgcatcag acgaggaaca attacctggt catgggggcc
    17461 aaacaaacca cggggacgca aaagaataaa tctcgtggca gaatatcgag aaacagcgcc
    17521 agcaataacc tgctcagcgg catatttact gctggcataa tggctggaga atgctcgcgc
    17581 aagataagtt tctggcaggt catagtgatg ttgaaaatca aagtaaaccg caggcgtgga
    17641 gatatggata aaccggggaa tattcagcct gcctgccgcc tgtgccagag tgtgggtcac
    17701 tttgacattg gcaagccaga acgccgtttt gtcgccccag ggcgaggact ttgccgcaca
    17761 gtgccagacg gcatcgcagc cgtccatcag gcgggcgcag tcatcctcgg ttgcctcggt
    17821 taaatcgaga gccacaaact gtgcgcctgc gtttgtcagg cttctgccaa cctcacaatt
    17881 tcgccctgtt gccacaaccg actcacccga ttgcaacaaa tattgcacgg catttcgccc
    17941 aaggccgctg gtcgcccctg ttacgaggat cttcacggta aaagcaccat gccagccagc
    18001 gttagtcccg ctgccgtgcc aatcaacatt acaggtttat ccgctgtgaa acgaccgcta
    18061 atcactgctt catgcagcgc cgtggggata gaggccgcga cctgattgcc gtaaaaacga
    18121 tacacatcga tgagcgcaga ttccggcact cgcagccgtt tacgcatatg ttcaagggaa
    18181 agatgactgg cctgatgcgg aacgaccgtg ccgatatcag acagttgcag gctgcttttt
    18241 tgcagcaacc gagaaaaata atcttcaata agcgaagaag caaaacgaaa cagcggtttc
    18301 ccctgcatat gaaaaagaaa gtcgctatca ctcacaccag agcgaggatt acgccgcgta
    18361 ccgccagcac gaatttcaca cagttcgctg ccttcgggat acatttcgat caggctagcg
    18421 ataatcccgc tgcgcccatc gccccgctca acaatcgcgc acgccgcgcc atcaccaaaa
    18481 attaatgaag actcttcatg atcccagtcg ataccgcgtg aagcaatatc tgccgaaacg
    18541 atggcaatgc gtcgatatgc tccacagttt aataatccgg cagcgacttc aagagcggaa
    18601 ataaagctca cacagctact gttgatatca aacccggcaa cgcctacggg catggctgcg
    18661 gctttaagaa tatgaattgc gctacagggt aatgcctgta cggcaatggc cgatgcacaa
    18721 attagcagat caatatcaga aggatttaag gctgccgcta ctaacgcatt atgtaaagcc
    18781 tctgccgcca gttcggcctg gctggcgtcg ttgcgggcaa aatagcgcca gcaaataccg
    18841 gaacgtcgct caacatagcc aactggctta tttaactgaa catccagttg tttagaagtg
    18901 atttgatcag ggggtaaggc gatccccgtg gcgattattt ttaacgccgc ggagatcgtc
    18961 ggttgattag gtcgagccac ggtcagtgct ccttcactgc tccataggtg gaccaatcat
    19021 atagatgaag tacgtttccc tacaatccga aaaatcggca gtgtgtaacc gccgatgttt
    19081 ggtttagata atgtttggtt tagataatta gtgacgagcg cgcaccagct gatacttacg
    19141 cgtcagatac tccaccggga cactccagat gtgtaccaga cgcgagaacg ggaacagcag
    19201 gaataacgtc atccccaaca ccaggtgcag acggaagata aacgccacgc catcaagatg
    19261 ttgagaagcg ccaccgtgga aggtcactac cgactgcgcc cagccaacca gtttcatcat
    19321 ctcgctaccg tccatatgct gagcagagaa cggaatggtc agcaggccca gcgcgcactg
    19381 gataacgagc agcgacagga tcaggatatc cgctccggta gtcgttgcac gcacgcgtgg
    19441 gctgaacaga cggcgtttca acaacagcac gccgccaatc agacacagta cgccgctggc
    19501 accaccagca aacatcgcca ttttctgctt cacttcaatc ggcagccagg cttcatacat
    19561 ccagtgcggc gtcagcatac cgaagaagtg accgacaaaa atccccagaa taccgatatg
    19621 gaacaggttc gacgccagat tcatcccttt gcgatccagc atctggctgg acgccgcgcg
    19681 ccaggtatac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
    19741 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
    19801 gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg
    19861 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagact
    19921 gcatccagcg cctgcggcgt atcgtcgcgc gcttcgtcgg caattttttc cgccactttg
    19981 tcgctgtcga ttgcggtatt cgccagtttc agcagcagat caaacagcac ggcataacgg
    20041 ctttcacgct gttgcagacg cgcactcagc aatgccagaa tcggcgcaat atctttcaaa
    20101 ccctccacgg cttcgctttg cggcagctgc gccaggtact ccagatacag cggcagatgg
    20161 tcgggcaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcagatcc
    20221 accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac
    20281 gtggcgcggc cacggtcgaa cagttcgctg tactgcgcct gggcatcgag cggctccatc
    20341 gtcgttaaat cgcgcaggaa aatgcccagc gcatgggcat cctcttttgg cagatttttc
    20401 gacgcggcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
    20461 tcaaggagac gcgatacaat cacgagttcg atcatgggtg cggctccgtt ttgctggtta
    20521 cgtcgatggc atcgatacgg cggctgttga acagattgaa tttggtatct gaaccgtggc
    20581 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgcgccagtt
    20641 cacgatgact gctcggcacc acaaagcgat cttcgtagtt ggcaatagcc agataacgat
    20701 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcgactt
    20761 taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagcgcgc
    20821 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
    20881 gcaggctttc gacgtctggc agaatgccgt tgctgcccag ttcacccgcg tctgccgcag
    20941 actgaatcgg cgacagaggc ggcacgtacc agaccatcgg caatgtgcga tattccggat
    21001 gcagcggcag cgccagtttc cattccatcg ccattttata aactggcgac tgctgcgcag
    21061 cctcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
    21121 tcggatcgag gaacacatcc agctgacgct ggtagagatc tttctcgttc tcggtacttg
    21181 ccgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga
    21241 cacaggtttc tgaacatatt gtcggctggc cggcttcaat acgcggatag cagaagatgc
    21301 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga
    21361 tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgccg tcttcttcac
    21421 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcaga cagtgttcgc
    21481 acaggcgcgg caaatacatc atgaaggtgt tttcgaactg gctatacatc gccttctgga
    21541 tgttgtcgaa gttcttgtct ttcgccagct tgtcaaactc accgcccagg tcatcttccc
    21601 agttcggccc tttttcgatt ttcgccatcc gttcgccggt aatcagcgaa cgcggacggg
    21661 caatcggctg tgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcaa
    21721 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt ttacccagca
    21781 gcatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg atccagccgc
    21841 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctggcccggc ttggtttcca
    21901 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtca
    21961 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
    22021 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
    22081 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
    22141 gttaaagcca tatgccagat gggcatagcc gccgatcata tgcgtcggtt tcggcgtgat
    22201 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
    22261 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
    22321 aaccgcacgg gcagtcagtg caccgttgct gttgaagact tcaatccagt cgttatcggc
    22381 gatacccagc tctttggcat cggcttcact cagccagacc accggaccac cgcgacctaa
    22441 agtcagcatc aacaggttgt cgctgtaggt ggagtggata ccccacttct ggtgtggcgt
    22501 caggaagttg agcgcttttt ccgggttgcc attggatttc tggcccatca cttctttcac
    22561 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
    22621 ttggtgatcc tgatacagct gctgacggcc agagagcgta cgccacggga tcagctcgtg
    22681 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tcaaggccag accatgtcgg
    22741 gctggagatg attttgcgcg gctgtgcctg aatatcgcgg aagcgaatct tctcgtcttc
    22801 tttattcagc gccagatgcg tatggtcacg gccggtaaac tcgctcaggg cagcccaggc
    22861 ttttacagct acctgaccgt tggtttccgg tgccagcgtc aggatcatct ctgccgcatc
    22921 aattgcggta ttgagcatcg gctgaccttt tgccggacct tccgcttttg tgtagttgag
    22981 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc
    23041 gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagttgccg gataatcgcg
    23101 ctctacgacc ataatgtgcg gtgcggtttt acctgggatc aggtcgcatt cgcctttttt
    23161 ccagtctttc acatccagcg gttgcgccag ttcagcggca gagtcgtgct ggataggcag
    23221 cgtgacgatg tcagtttctt tacccagatg accaacgcac acttcggaga atttcttcgc
    23281 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
    23341 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
    23401 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
    23461 caccaccaga tccagcttgc cttccagacc gttgtcctgc cagtccactt cttccggctt
    23521 cacgccgccc tgctgcccca ggtctttgcc ctggataccg tgctccgtcc ccagcaggta
    23581 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
    23641 caggttacgc gggtggtttt taccgttttc cggctgttcc gcagcgaaac ggatagaacc
    23701 ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt ctgcttcgcg
    23761 agcgatagtc agcgggttag tgcctaactg cggcgcagac ggcagccagc ccatgcgttc
    23821 tgcacggacg ttaaagtcga tcaagtgtcc ggtatagcga gatttatccg ccatcggcga
    23881 cagcagttct tccgcagtca cggtttcata acgccactgg ctggagtggt tatagaaata
    23941 agaggtactg ttcatgtgac gcgccggacg ctgccagtcg agggcaaacg ccagcggctg
    24001 ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
    24061 ctgaccgaca caaccgcaga aaatcagcat gttgatcaga ccacgatagt tcatgtccag
    24121 gtgataccag tggttcagcc ctgcgccgac gataatcatc gaacgaccgt gcgttttatc
    24181 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
    24241 ctgctctgcc caggcagggg tgtaagcttt cacatcgtca tagctggttg cacagttaac
    24301 gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
    24361 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagcttat gcagcagcac
    24421 gttttccagt tccactttgt tgaagtgttc cgtgccatcg ccaccaaagt acgggaagcc
    24481 cacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg tttcttcgcc
    24541 agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
    24601 agaaccgttc ggcgcaacca tttcaccatt ggtattaaag gcgacagttt tccattccgg
    24661 attgttttcc tggcccagtg catccaccag atcagcagcg cgcagcatac gacccgcagc
    24721 gtagtaaccg tcgcgctctt ccagcatcac cagcatcggc atgtcggtgt agcggcgcac
    24781 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtgacc
    24841 cattgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
    24901 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtccctt tgtaacgcac
    24961 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac cccaggcgat
    25021 gatgtagctg gagttgtacc agtcagcaga ttccggtacg tcagtttgct caccccaggt
    25081 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
    25141 aatcagcgag agatagcgcg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
    25201 cgagaagcca gcgacacggt ccgggccgta atttttgatg gtgtaaacgt tagatgcggc
    25261 gatcagttcg ttcacttcct gccaggaaga acgaacaaaa ccaccgcgtc cacgcgcctg
    25321 cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
    25381 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
    25441 tttcaggcgg ttagcgctgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
    25501 aggttcatgg tttggcaggt ccgggcgagt acgcggatag tcagtctgct gggtttccca
    25561 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
    25621 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
    25681 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
    25741 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
    25801 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
    25861 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctgg aatacccc
//
LOCUS       DAJOTB010000059        25903 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJOTB010000059 DAJOTB010000000
VERSION     DAJOTB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.059"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            82..1737
                     /gene="opgD"
                     /locus_tag="OHN28_002385"
                     /gene_synonym="mdoD"
     CDS             82..1737
                     /gene="opgD"
                     /locus_tag="OHN28_002385"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCQ2297775.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            1877..2101
                     /locus_tag="OHN28_002386"
     CDS             1877..2101
                     /locus_tag="OHN28_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCQ2297776.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            2164..2703
                     /gene="rimL"
                     /locus_tag="OHN28_002387"
     CDS             2164..2703
                     /gene="rimL"
                     /locus_tag="OHN28_002387"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCQ2297777.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(2695..3675)
                     /locus_tag="OHN28_002388"
     CDS             complement(2695..3675)
                     /locus_tag="OHN28_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCQ2297778.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            3799..4791
                     /gene="tehA"
                     /locus_tag="OHN28_002389"
     CDS             3799..4791
                     /gene="tehA"
                     /locus_tag="OHN28_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCQ2297779.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            4788..5381
                     /gene="tehB"
                     /locus_tag="OHN28_002390"
     CDS             4788..5381
                     /gene="tehB"
                     /locus_tag="OHN28_002390"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCQ2297780.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5684..6352
                     /locus_tag="OHN28_002391"
     CDS             5684..6352
                     /locus_tag="OHN28_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCQ2297781.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6387..7598)
                     /gene="ydcO"
                     /locus_tag="OHN28_002392"
     CDS             complement(6387..7598)
                     /gene="ydcO"
                     /locus_tag="OHN28_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCQ2297782.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            7651..8187
                     /gene="sutR"
                     /locus_tag="OHN28_002393"
     CDS             7651..8187
                     /gene="sutR"
                     /locus_tag="OHN28_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCQ2297783.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            8260..10221
                     /gene="rlhA"
                     /locus_tag="OHN28_002394"
     CDS             8260..10221
                     /gene="rlhA"
                     /locus_tag="OHN28_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCQ2297784.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(10313..10543)
                     /locus_tag="OHN28_002395"
     CDS             complement(10313..10543)
                     /locus_tag="OHN28_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HCQ2297785.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            10722..10958
                     /gene="hicB"
                     /locus_tag="OHN28_002396"
                     /pseudo
     CDS             10722..10958
                     /gene="hicB"
                     /locus_tag="OHN28_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            11037..12443
                     /locus_tag="OHN28_002397"
     CDS             11037..12443
                     /locus_tag="OHN28_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCQ2297786.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            12688..13833
                     /locus_tag="OHN28_002398"
     CDS             12688..13833
                     /locus_tag="OHN28_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2297787.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            13851..14864
                     /locus_tag="OHN28_002399"
     CDS             13851..14864
                     /locus_tag="OHN28_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2297788.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            14865..15806
                     /locus_tag="OHN28_002400"
     CDS             14865..15806
                     /locus_tag="OHN28_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297789.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            15796..16590
                     /locus_tag="OHN28_002401"
     CDS             15796..16590
                     /locus_tag="OHN28_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2297790.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            16612..18036
                     /gene="patD"
                     /locus_tag="OHN28_002402"
     CDS             16612..18036
                     /gene="patD"
                     /locus_tag="OHN28_002402"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCQ2297791.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(18133..18228)
                     /gene="yncL"
                     /locus_tag="OHN28_002403"
     CDS             complement(18133..18228)
                     /gene="yncL"
                     /locus_tag="OHN28_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCQ2297792.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            18424..18597
                     /gene="ortT"
                     /locus_tag="OHN28_002404"
     CDS             18424..18597
                     /gene="ortT"
                     /locus_tag="OHN28_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCQ2297793.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            18683..18916
                     /locus_tag="OHN28_002405"
     CDS             18683..18916
                     /locus_tag="OHN28_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCQ2297794.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTL
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(18917..19366)
                     /locus_tag="OHN28_002406"
     CDS             complement(18917..19366)
                     /locus_tag="OHN28_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2297795.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(19363..19881)
                     /gene="mddA"
                     /locus_tag="OHN28_002407"
     CDS             complement(19363..19881)
                     /gene="mddA"
                     /locus_tag="OHN28_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCQ2297796.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            20130..20285
                     /locus_tag="OHN28_002408"
                     /pseudo
     CDS             20130..20285
                     /locus_tag="OHN28_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(20362..22464)
                     /locus_tag="OHN28_002409"
     CDS             complement(20362..22464)
                     /locus_tag="OHN28_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCQ2297797.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            22706..23767
                     /locus_tag="OHN28_002410"
     CDS             22706..23767
                     /locus_tag="OHN28_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCQ2297798.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(23880..25379)
                     /gene="ansP"
                     /locus_tag="OHN28_002411"
     CDS             complement(23880..25379)
                     /gene="ansP"
                     /locus_tag="OHN28_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCQ2297799.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
BASE COUNT         6560 a         6380 c         6575 g         6388 t
ORIGIN      
        1 ggtcataaat aagcactttc ttgccgctga aaatgaccag cgcgggacca ttcacaacac
       61 cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc aatggctatg
      121 gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc attcgcggcc
      181 gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct acagtcaatg
      241 gcgcacgact tagcgcaaac agcatggcgt ggtgcaccac gtccgttacc tgatactctg
      301 gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa atcgctctgg
      361 cataacgttg agaaccgtca actggacgct cagtttttcc atatgggaat gggattccgt
      421 cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga aattcacttt
      481 cgcccggagt tgttcaaata caacgatgcg ggtgtggata ccaaacaatt agaagggcaa
      541 agcgatctcg gttttgccgg ttttcgcgtg tttaaagccc ccgaactggc gcgccgtgat
      601 gtagtatcat tcctcggcgc gagttatttc cgcgccgttg atgacacata tcaatacggt
      661 ttgtcggccc gcggcctggc gatcgacact tacaccgaca gcaaagaaga gttccccgac
      721 tttaccgcct tctggtttga tacggtaaaa ccaggggcaa ccacctttac cgtttatgcg
      781 ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg tgagaaaaat
      841 caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa acagctgggc
      901 attgcaccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat gtgcgacacc
      961 attcatccgc aaatccatga ctctgatcgt ctgtccatgt ggcggggcaa cggcgagtgg
     1021 atttgccgtc cgctgaacaa tccgcaaaaa ttgcagttca atgcttacac cgacaacaac
     1081 ccgaaagggt ttggtttatt gcaactggat cgtgatttct cccattatca ggacattatg
     1141 ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg gggtaagggc
     1201 accatcggcc tgatggaaat cccaactacg ggggaaacgc tggataacat tgtttgcttc
     1261 tggcagccag aaaaagctgt aaaagcgggt gatgagtttg cattccagta tcgtctgtac
     1321 tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac gcgtaccggc
     1381 atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa atgggcgcga
     1441 cgttttgccg tcgatttcgt tggtggcgat ctgaaagctg ccgcgccaaa aggcattgag
     1501 ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta tattgaaccc
     1561 attgatggtt atcgtattca gtttgactgg tatccgactt cggactccac tgatccggtc
     1621 gatatgcgga tgtatctgcg ttgtcagggc gacgctatca gtgaaacatg gttatatcag
     1681 tatttcccgc cagcgccgga taaacgtcag tatgttgatg accgcgtgat gagttaatcg
     1741 ttttttcttc ggcatcttct tcgggaggtg ccgtctggtt aaacacgatc ccgctcgcat
     1801 tttttcctaa gttaaatgag taatctgctg gtgtgtattt cagatacacc ttgtcagcca
     1861 ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc tgaaaaacga
     1921 aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg aaattgccag
     1981 aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga aaaaacaaaa
     2041 gctacagtta aaagatgaga tgctcaaaat cctgcagcag gtgagcgtca aagaggtgta
     2101 aactttccta agccgccagc caggcggctt ttttaacagc tgcatggatt gactggagat
     2161 aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt tgcggaaagt
     2221 catgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca gcagtcgcta
     2281 aactggccgc agtttgttca aagtgaagag gacacgcgaa aaacggtgca gggtaatgtg
     2341 atgttgcatc aacgcggcta tgccaaaatg ttcatgattt tcaaagaaga tgaacttatc
     2401 ggcgttatct cttttaatcg tattgaacca ctgaataaaa ccgctgaaat cggctactgg
     2461 ctggacgaac ctcatcaggg gcaggggatc atttctcagg cgctgcaggc attgattcat
     2521 cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt ggacaatccg
     2581 caaagcaacc aggtagcgtt gcgcaatggt tttatccttg aaggttgcct gaaacaggct
     2641 gagtttctga atgatgccta tgatgatgtg aacttatacg cgcgtattat cgattcacaa
     2701 taaccctgcc agcagcgttc gcgtaatgcg ctcctcgccg ttaatgactt ttggaccacg
     2761 gacatgaatc gtgtcgccat caaaggcttc aacgacggcg tggtcagtta actccacatg
     2821 gttttcaatt atcaccgcac ctgtaatacg gctctcacct tgtattacaa cgtgctcgtc
     2881 gagcagaatc ggtccgccac gtaccacggc atttccgcca atcaagacat gatgttttaa
     2941 cacacaatta ccttccacaa tggcatattc cgccacctgt gaactgtaat gaatcgtcgg
     3001 aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtaaactt tagcgcaatc
     3061 gcatagccag acgttgttct cttcattacc ttcaatactg gcaaaatcaa aaacttcggc
     3121 gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg cctggtggac
     3181 aatacgcgag gcgctaacct ttgcgcgatc ataaatttgt agcaggagtt gatggtcggg
     3241 cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt gaccaaatat
     3301 tctacattga ccgcaaacta atgagtcact aatggtgaca ctgtcactta tatatgcacc
     3361 ctggctgatt tcactattat cgatccagac attatccgtt gcgtaaacct ctccccacag
     3421 cacgctggtg ccggtgatac gcgtattccc ggaaatcacc gttccgccga acgcaatagc
     3481 attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc gatcgatcca
     3541 gccgccagcg cttcctgcta tcacatcgtt aaaatcgctc atggcgataa tttgccgtaa
     3601 taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt cttcacttag
     3661 acgatattta cgcatagcgt tttcccacag gtgacttact ataaccgtag caaattctgc
     3721 ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact ttataaatta
     3781 aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc aggctacttt
     3841 ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag ccaggtttgg
     3901 caggtcagcc actggttggg ggatgggctg gtgattctgg cgatgatcat ctggggatta
     3961 ttgactggcg catttattac ccgactcata cgctttccgc atagcgtgct ggcagaagtg
     4021 cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat gctggtggcg
     4081 attggttttg ttccgtggtt tcgcccactg gcggtgtgcc tgttcagttt tggtgtcgtg
     4141 gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgtggatc tcaccctgaa
     4201 gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat cagcgcaatg
     4261 gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc aggcgttttc
     4321 tcatggctaa gcctggaacc ggtgatcttg cagcgtctgc gcagttcggg agaattaccc
     4381 acggcactga ggacatcact cggcattcag ctcgctcctg cgctggtggc ctgtagtgcc
     4441 tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt cggttatgga
     4501 ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca gccatttaat
     4561 gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg tttgcatctg
     4621 gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt tatctttacc
     4681 aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca gggaaaattg
     4741 ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg atcattcgtg
     4801 atgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa gtgctggaag
     4861 cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat ggtcgtaaca
     4921 gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat gccatgagta
     4981 tcgccaacgt cgagcgcatt aagtccattg aaaatctgga taatttacac acccgggtcg
     5041 ttgatctgaa taacctcaca tttgatggac agtacgattt tattctttcg actgtggtgc
     5101 tgatgttcct tgaggctaaa accatcccag ggctgattgc caatatgcaa cgttgcgcta
     5161 aacctggcgg ttacaacctg attgtggcgg cgatggatac cgctgattat ccatgtaccg
     5221 tcggcttccc gtttgccttc aaagaggggg aattacgtcg atattacgaa ggctgggaga
     5281 tggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac ggtaatcgta
     5341 ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg atccggtaag aacaaagcgc
     5401 gtgaaaattc cccacgctga gatggtttac tgttcttctt ttcggtaagc atatttttta
     5461 tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagccgctta acgtactgaa
     5521 atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc gtctgaaaag
     5581 gggctaaaaa atttattttc accaacactt tttttgccac aacacgaagc ggcgcttttt
     5641 gctataactt aaaaagtaat ataatcatct caggaaacta ttcatgcgta ccacatcatt
     5701 tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct ctaaaatcac
     5761 ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag aaacaacctc
     5821 ggcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa gcaaatatga
     5881 cagcatcgtc tggaacccaa tcacttatta tccggtaccg aaaccttcta cccaggtagg
     5941 gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag aagcgatagc
     6001 gcagcgtaaa ccactggtta ccaccgctgg gccgcgtagt ctgattttcc gtggggccat
     6061 taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc ctgttgcatt
     6121 agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc gcttgtattt
     6181 tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg ttcgtcaggg
     6241 cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata ttaaacaagt
     6301 tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt agttccagac
     6361 gcgccatcgt tagatggcgc tttttattat cggtttctgt tagcgattaa attcaacacc
     6421 acgtaacaaa cgcctccggc aattaatccc caaaacgcag aaccaatccc gaacagcgtc
     6481 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
     6541 agcgcctgat acaaactgcc gccaatggta ctcaacagcg caagacctgc cagcatctgg
     6601 atccaactta cgggcagggc agccatcatc ccggtaatgg cactaccaaa cagacctgcg
     6661 atcaaataaa aaatgccggc aacggcagcg gccagccaac gttgatcttt atccggatgc
     6721 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
     6781 aaaggggaaa aaaacagtgc cagcaatcca gtaaacacaa tcaacggcga aacaggagcc
     6841 gaatacccgg ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc catcgtcacc
     6901 agaaaaaggg ggagtgcaac gctcagactg tgagcaaacg aaaaatcagg ggtaatataa
     6961 gtggggagaa cgggtttaaa gacaatatca gttgtaacaa cgtcaccttg cgcggtaacg
     7021 atgacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggct
     7081 gccagccatg ccagcaacat actgccacac aacgtaaatt gaccatccag actggcaaac
     7141 gcctgtaaac caaaacgtaa taaaatcccg gcaagcattg ccgccgcaag cgagtgcgga
     7201 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
     7261 gtgacaataa aaacgccgat ggcttcgtta agtgttagtt cctgcaatcc ggtgaccaac
     7321 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
     7381 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
     7441 gtggtggctc cggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
     7501 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata
     7561 gtcacctcgt gcgttataac gtacaggtac cgtagcattt gtccgttata acgcacaagt
     7621 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgttt tttatccacc
     7681 acacttaaac aactacgtca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
     7741 ggcgtttcta aagcgatgtt agggcagatt gagcgtaatg aatccagccc gacggtcgcg
     7801 acattatgga aaattgccac cgggttgaac gtgccatttt caacatttat ttctccgccg
     7861 cagtccgcta cgccttcagt ttatgatcca caacggcagg cgatggtgat cacttcgctg
     7921 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc acccggtgca
     7981 ataagcgaat cgatgcccca tgaaaagggg gtaatcgaac atgtcgtcgt cattgatgga
     8041 caacttgatc tgtgtgttga tggtgaatgg cagagcctta attgtgggga aggcgttcga
     8101 tttgctgcag acgtcacgca tgtctatcgt aatggtgggg agcaaaccgt acattttcat
     8161 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
     8221 gctaaaatag ccgccatttt tcagctactg gataagaaag tgaccgtatc ttctcatcgt
     8281 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
     8341 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
     8401 ctgaaagata ttgccgagtt ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
     8461 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
     8521 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
     8581 attccgccga ttgaactgca cgccagtacg cagtgcgata ttcgtacagt agaaaaagcg
     8641 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagaact gaatcttgag
     8701 cagatccgcg cgattcacca ggccacgaac gcgaccattg aattctttat tcatggcgca
     8761 ttgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
     8821 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
     8881 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac
     8941 ctcggcgcgc tgattgatgc tggcgtacgc tccttcaaga ttgaagggcg ttacaaagat
     9001 atgagctacg tgaagaatat caccgcccat tatcgccaga tgctggatgc cattattgaa
     9061 gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg
     9121 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
     9181 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggcgaagt attgaaagta
     9241 gcgaaagatc atattgatgt tgccgttacc gagccactgg caaatggcga tggcctgaac
     9301 gtgatgatta aacgtgaagt cgtcggtttt cgtgccaata cggtagagaa aaccggagaa
     9361 aatcagtacc gcgtctggcc aaatgaaatg ccagcagatt tgcacaaaat tcgcccgcat
     9421 cacccactaa accgtaatct tgatcataac tggcaccagg cactgacaaa aacctccagc
     9481 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
     9541 ctgaccagtg aagagggcgt cagcgtcacg catacgctgg acgggcagtt cgacgaagcc
     9601 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tgacaaaact ggggcaaacc
     9661 atctattacg cccgcaatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
     9721 ctgttaaacc agttccgccg tgaagccgcc gatatgcttg atgctgcgcg tcttgccagt
     9781 taccagcgcg gcagccgtaa accggttgct gatcctgcgc cggtttatcc gcaaacgcat
     9841 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
     9901 gtgcaactga ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg
     9961 atcaccaagc attgtctgcg ctttgccttc aatctgtgcc cgaaacaggc gaaaggcaat
    10021 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
    10081 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
    10141 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgatct gctgaaaaca
    10201 ttgccgaagc gaaaagggta aaacgccagt tttcgggtta ctcaccactc atcaaatctg
    10261 catgatattg cctgccgggt aaggcgtcat gccgcatccg gcaacaaatg actcaacgct
    10321 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
    10381 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
    10441 cattctttac ccacataaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
    10501 gtgcacacgt taataagaca accgataacg ccttcgaaaa catcccgcca cctttttcta
    10561 accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt tgcaaattag
    10621 agcaaagaaa ttctggaatc ttccttcctg attttgcatt gaattctgcc gttgcggcga
    10681 tttagtgcta ttttcgagca cattacacac ggaggtaaac ttcgccatta aatagtcacg
    10741 atcactttat tgaagtgcct ttgagcgtcg cctctaatgt attgctgtta aatgcttttt
    10801 tacagtcaga aatcactcag caagagttag ccaggcgaat tggcaaacat aaacaggaga
    10861 ttactcgcct atttaatttg caccatgcga caaaaatcga cgccgtccag ctcgcggcta
    10921 aggcgcttgg caaagagtta tcgctggtga tggtttaatt acagttaacg aaaagttgtc
    10981 atttttaaca actgatatag actgccgaat catctgcaca taattacgat tcgataatga
    11041 aaaaatacca gcagcttgca gaacaattac gcgagcagat tgcgtcgggt atctggcaac
    11101 ccggcgatcg tttgccttcg ttgcgtgacc aggtggcgct ttctggcatg agctttatga
    11161 ctgtcagcca tgcctatcag ttgctcgaaa gtcagggata tattatcgcg cgaccgcaat
    11221 cgggttatta cgttgcgcca caggcaataa aaatgccgaa agcgacagtc attccagtca
    11281 cacgagatga agcagtcgat atcaacacct atatttttga tatgttgcag gccagtcgcg
    11341 atccgtcggt agttccgttt gcctcggcct ttcccgaccc gcgacttttc cccctccaac
    11401 aactaaaccg ctcgctggcg caggtaagca aaaccgccac ggcgatgagc gtgattgaaa
    11461 acttaccgcc aggaaacgca gaactgcgtc aggctattgc tcgtcgctat gccttacagg
    11521 gcatcaccat ttctcctgat gaaattgtca ttaccgccgg ggcgttagag gcattaaacc
    11581 tcagtttgca agcggtaact gaaccgggcg attgggtgat agtagagaat ccttgtttct
    11641 acggcgcgtt gcaggcgctg gagcggttac ggctgaaggc gttatcggta gcgacggata
    11701 ttaaagaagg gatcgatctt caggcgctgg aactggcgtt gcaggagtat ccggtgaaag
    11761 cgtgctggct gatgactaat agccagaacc cacttggatt taccttaacg ccgcagaaaa
    11821 aagtgcggct ggtagcgttg ctcaatcagt acaacgtaac gctgattgaa gatgacgttt
    11881 atagcgaact ttattttgga cgggaaaaac cgctgcctgc gaaagcgtgg gatcgccacg
    11941 atggtgtttt gcattgctct tcgttttcga aatgtctggt gcctggtttt cgtattggtt
    12001 gggtcgccgc cggaaaacat gcacgtaaaa ttcaacgctt gcagttgatg agtacgcttt
    12061 ccaccagctc accgatgcag cttgcgctgg tggattacct ttctactcgc cgatacgatg
    12121 ctcatcttcg tcgcctgcgt cgccagcttg cggaacgtaa acaacgcgcc tggcaggctt
    12181 tgctgcgtta tctgcctgcg gaagtcaaaa ttcatcacaa tgacagcggt tattttctct
    12241 ggctggagct ccccgagccg ttagatgcag gagaattaag cctggtggca ctgacgcatc
    12301 atatcagtat tgcgccgggt aatatgtttt ctaccggtga aaactggtca cgtttttttc
    12361 gttttaatac cgcgtggcag tggggagaac gtgaagaaca ggcggtaaaa caattaggca
    12421 aacttattca agaacggctg taatagcgtt taatttaatt cctctcaggt tgggtaatat
    12481 gaatttcgaa tagcagtcat atatcctaac tccttgtcta tactccagaa gataacctta
    12541 cagacggcat aatgcgcggt agctcacaac ctgattaaat tttctcaggg ccgaaggtgt
    12601 gcctgcaagc cgccgtctat ggttaaacaa ggagatattt ttacggcacg gcggctgaac
    12661 aattaattac gacaggagta agaccttatg agcaagacat ttgcccgcag cagcctgtgt
    12721 gcgctcagca tgacaataat gaccgctcac gccgccgaac cgcctaccaa tttagagaat
    12781 ccggaagggc gactggatat tatcgcctgg ccgggataca tcgaacgcgg acaaactgat
    12841 aaacagtacg actgggtaac gcaattcgaa aaagagacag gctgcgcggt gaatgttaaa
    12901 accgccgcga cttccgatga aatggtcagt ctgatggcca aagggggtta cgatctggtt
    12961 acggcatccg gcgatgcctc gctgcgtttg attatgggca aacgcgtgca gccaattaat
    13021 accgccttga ttcccaactg gaaaacgctc gatccgcgcg tggttaaagg cgactggttt
    13081 aacgttagtg gcaaagttta tggcacacct taccaatggg gtccgaacct gttgatgtac
    13141 aacactaaaa ccttcccgac gccgccggat agctggcaag tggtttttgt tgagcagaat
    13201 ctgccggacg gcaagagcaa taaaggccgc gttcaggctt atgatggccc tatctacatt
    13261 gcggacgctg cgttgttcgt taaagccact cagccgcagt tgggcatcag tgatccgtat
    13321 caactcaccg aagaacagta ccaggcggtg ctgaaagtgc tgcgcgatca acatagtttg
    13381 atccatcgct actggcatga cactaccgtg caaatgagcg atttcaaaaa cgagggtgtg
    13441 gttgcttcca gtgcatggcc ctatcaggcc aacgccctga aagccgaagg ccagcctgtc
    13501 gctaccgttt tcccgaagga gggcgttacc ggttgggctg acaccaccat gctacatagc
    13561 gaagcgaaac atccggtttg cgcctacaaa tggatgaact ggtcattaac cccaaaagtg
    13621 cagggcgatg tggcggcctg gtttggctcg ttaccagtag tgccgcaagg gtgtaaagcc
    13681 agtccgttat taggcgagaa aggttgtgaa acaaacggtt ttaactattt cgataaaatc
    13741 gccttctgga aaacgcctgt agcagaaggg ggcaagtttg ttccctacag tcgctggacg
    13801 caggattaca ttgccattat gggcggtcgc taacttcgct ggggtgtttt atgacgtacg
    13861 cagtggagtt tgacaacgtc tcacggttgt acggtgacgt gcgcgcagta gatggcgtca
    13921 gtattgcgat aaaagatggt gagttcttct ctatgctggg gccgtccggc tccggcaaaa
    13981 ccacttgcct gcgcctgatt gctggattcg aacagctttc cggcggggct atctctattt
    14041 ttggtacgcc cgccagcaat ctgccaccgt gggagcggga cgtgaatact gtcttccagg
    14101 actacgcgct atttccgcat atgtcgattc ttgacaacgt agcctacggg ctgatggtca
    14161 aaggcgtgaa taaaaagcag cggcatgcaa tggcgcaaga ggcgcttgag aaagtggcgt
    14221 tggggtttgt acagcaacgt aaaccgtcac aactttctgg tggtcagcgc cagcgggttg
    14281 ctatcgccag agcactggtg aacgaaccgc gtgtgttgtt gctggatgaa ccgctcggcg
    14341 cgctggatct caaattgcga gagcaaatgc agcttgagct gaaaaagctg caacagtctc
    14401 tcggtatcac ttttatcttc gttacccacg atcagggcga agcgttatcg atgtccgatc
    14461 gggtggcggt tttcaataat ggacgcattg aacaggtcga ttctccgcgc gatctctata
    14521 tgcgcccgcg cacgccgttt gttgcgggat tcgtcgggac atccaacgtt tttgatccgc
    14581 taatggccga gaaactttgt ggcatgacgg gaagcttcgc actacgaccg gaacatatcc
    14641 gcctcaacac ccatggcgaa atgcaggcta atggcacgat ccaggcggtg caatatcagg
    14701 gcgcagcgac ccgttttgaa ctgaaattga acggcggtga aaaactgctg gtgagtcagg
    14761 ccaatatgac aggcgaagag ctacctgcca cgctcacgcc cggacaacag gtgatggttt
    14821 cctggtcacg tgatgtgatg gtggcgctgg ttgaggagag gtgaatggcg atgaacgtat
    14881 tgcaatcacc ttcacgtcca ggtctgggta aggtgtccgg tttcttctgg cgtaatccgg
    14941 ggctggggct gtttttgctg ctgcttggcc cgctaatgtg gtttggcatt gtctatttcg
    15001 gctcgctgct gacgctgtta tggcagggct tttatacctt tgacgatttc accatgtcgg
    15061 taacgccgga actgacgctg gcgaatatcc gcgcgctgtt taatccggcg aattacgaca
    15121 tcattgtccg cacgctgacc atggctgtgg cagtcactat cgccagcgcc attctggctt
    15181 ttccaatggc gtggtatatg gcgcgctata ccagcgggaa aatgaaagcg tttttttata
    15241 tcgcggtaat gttgccgatg tgggcgagct acattgttaa agcctatgcc tggacgttat
    15301 tgctggcaaa agatggcgtg gcgcagtggt ttttacaaca tctggggctg gaaccactgc
    15361 tgactgcgtt cctcacgcta ccggcggtgg gaggtaatac gctgtcaact tccgggctgg
    15421 ggcgctttct ggtgtttctc tatatctggt tgccgttcat gatcctcccg gtgcaggcgg
    15481 cgcttgagcg tttgccgccg tcattgttgc aggcgtcggc tgatcttggc gcacgtccac
    15541 gacaaacctt tcgctatgtg gtgctgccgc tggcaatccc gggtattgcc gctggctcta
    15601 tctttacctt ctcactcacg ctgggcgatt ttattgtccc gcagctggtt ggccctccag
    15661 gatattttat cggcaatatg gtttattccc agcagggggc gattggcaat atgccgatgg
    15721 cggcggcatt caccctggtg ccgattgttc tcatcgcact ttacctggcg ttcgtgaaac
    15781 gtctgggagc gttcgatgca ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg
    15841 ggcggcgtgg ttttcctaca ttttcccatc ctgattatcg ctgcctatgc gtttaacact
    15901 gaagatgcgg cgtttagttt tccaccgcaa ggcctgacgc tgcgctggtt tagcgtggca
    15961 gcacagcgta gtgatatcct tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg
    16021 acattaatcg cgctggtgtt agggacgctg gcagctgccg cgctgtggcg acgagacttt
    16081 ttcggcaaaa acgccatttc gctgttactg ttgctgccca ttgcgctgcc gggcattgtc
    16141 actggtctgg cgctattaac cgcctttaaa accatcaatc tggagccagg atttttcacc
    16201 atcgtggtcg gtcatgcgac tttttgcgta gtggtggtgt ttaacaatgt catcgcccgt
    16261 tttcgccgca cctcgtggag tctggttgag gcgtcaatgg atcttggggc caatggctgg
    16321 caaaccttcc gctacgtagt gttgccgaat ctcagttcgg cgttactggc tggaggaatg
    16381 ctggcgtttg ccttgtcgtt cgatgaaatc atcgtgacga cttttacggc aggtcatgaa
    16441 cgaacgttac cgttgtggtt gctcaatcag cttgggcgac cgcgtgatgt accggtaacc
    16501 aacgtggtgg cactgctggt tatgttggta acaaccttgc cgatcctggg ggcctggtgg
    16561 ctaacccgcg aaggcgacaa tggtcaataa ccactgatac aggaatatgc tatgcaacat
    16621 aagttactga ttaacggaga actggttagc ggcgaagggg aaaaacagcc tgtctataat
    16681 ccggcaacgg gggacgtttt actggagatt gccgaggcat ccgcagagca ggtcaatgct
    16741 gctgtgcgcg cggcagatgc agcatttgcc gaatgggggc aaaccacgcc aaaagcgcgt
    16801 gcggaatgcc tgctgaaact ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa
    16861 ctggagtccc gtaattgtgg caaaccgctg catagtgcgt tcaatgatga aattccggcg
    16921 attgtcgatg tctttcgctt tttcgccggt gcggcgcgct gcctgaatgg tctggcggca
    16981 ggtgaatatc ttgaaggtca tacttcgatg atccgtcgcg atccgttggg ggtcgtggct
    17041 tctatcgcac cgtggaatta tccgctgatg atggctgcgt ggaaactggc tccggcgctg
    17101 gcggcaggga actgcgtagt gcttaaacca tcagaaatta ccccgctgac cgcgttgaag
    17161 ttggcagagc tggcgaaaga tatcttcccg gctggcgtga ttaacgtgct gtttggcaga
    17221 ggcaaaacgg tgggcgatcc gctgaccgga catcccaaag tgcggatggt gtcgctgacg
    17281 ggttctatcg ccactgggga acacatcatc agccataccg cgccgtccat taagcgtact
    17341 catatggaac ttggtggcaa agcgccagtg attgtttttg atgatgcgga tattgaagct
    17401 gtggtcgaag gtgtacgtac ttttggctat tacaatgccg gacaggattg tactgcggct
    17461 tgtcggatct acgcgcaaaa aggcatttac gatacgctgg tggaaaaact cggtgctgcg
    17521 gtggcaacgt taaaatcggg ttcgccggat gacgagtcta cggagcttgg acctttaagc
    17581 tcgctggcgc atctcgaacg cgtcagcaag gcggtagaag aggcgaaagc gacagggcac
    17641 atcaaagtga tcactggcgg tgaaaagcgc aagggtaatg gctattacta tgcgccgacg
    17701 ctgctggctg gcgcattaca ggatgatgcc atcgtgcaaa aagaggtatt tggtccggta
    17761 gtgagtgtta cgctcttcga cagcgaagaa caggtggtga actgggcgaa tgacagtcag
    17821 tacggacttg catcttcggt atggacgaaa gatgtgggca gggcgcatcg cgtcagcgcg
    17881 cggctgcaat atggttgtac ctgggtcaat acccatttca tgctggtaag tgaaatgccg
    17941 cacggtgggc agaaactttc tggttacggc aaggatatgt cactttatgg gctggaggat
    18001 tacaccgtcg tccgccacgt catggttaaa cattaaggat aatattgcag atcgtaagag
    18061 tatgagatga tcttgcgtac tgtcagagca ctgatttctc tgacagtacg tgctgcgtgc
    18121 aaaatcacta catcaaataa accagccaaa tctcatagag ataagagtaa acaaaccaac
    18181 agcagcaaag acatttatca gtactacagt tctactggaa acattcattt ttgccacctt
    18241 ttatcattgc ccctatgtgt atagtacggg ttaagaaaat ccgcaataat actcattctg
    18301 aagatggata agggcaagtt gctgtttgat gattttttct caaactggtg aaaaaaactt
    18361 gatgcacgtc aaaaaatgac gcatatttgc gcgcgtttta ttcatctggc tggacgcccg
    18421 tacatgtctc tctatcaaca catgcttgtt ttctatgcgg ttatggcagc aatcgcattt
    18481 cttatcacct ggtttctttc tcatgataag aaacgcatcc gtctcttaag cgcctttctg
    18541 gtgggggcaa catggccgat gagtttcccg gtggcgctgt tgttttctct gttttaaatc
    18601 ttatgcactg gcttgacggc accacgcaga cttatatcat ttggatgaat cgataaattt
    18661 cacaagtggc taaggagaaa gtatgtcgca tctggatgaa gtcatcgcgc gcgtggatgc
    18721 cgcgattgaa gagagcgtca ttgcccatat gaacgaatta ttaatcgccc tgagcgatga
    18781 cgcggagtta agtcgggaag atcgctacac cctgcagcag cgtttgcgca ccgctattgc
    18841 tcatcacggt cgcaaacata aagaagatat ggaagcgcgc cacgaacagt taaccaaagg
    18901 cggtaccatc ctctgattaa aatgagcgtc tggccaccag ccaggcgcca accacaagta
    18961 aaattgcgcc acaaattggg ccgatcaacg cccgaagtgg tacgccatgg ctgcggaaga
    19021 tatccagcac cagaccgcca atcagctggc tggcgaccag tactgcaata gttgtcgccg
    19081 ctcccacatt ctgatatccg ctgatactgg cgaagacaaa aaacgatccc agcaagccag
    19141 gtataagtgt ccaccagcgc acgctggaca ccagttcgcc aaaccccgcc atgccttgtt
    19201 taaaccataa aatagaaaca aagagcacaa tccccaccag tgagttcagc aacatggcga
    19261 tgagaatggt agaggaggtc tgggtgatgc gcaccatcag cgtgttctgg actaccagac
    19321 caatcccggc tgcaatcaaa aaggcaaggg taagtgactg attcatccaa tcgcgtccgg
    19381 ttcagtgcgc tcgtcgagtt gcaactgcat aaatgtcaga tccagccagc gcccaaattt
    19441 agtgcctacc tgcggcattt gcgcggtgac gacaaatccc agcgactggt gcagatgcag
    19501 cgaggcctga ttttgcgatt cgatcccggc gaccatgaca tgcttcccgc aatcccgcgc
    19561 ttcatcaatt aatcggctta acaatttacg ccccagacct ttcccctgat gatcgggatg
    19621 gacataaacc gaatgttcca cggtatggcg aaaaccatca aaactacgcc agtcgccaaa
    19681 cgaggcatat cccgtcacta cgccatcttc ttcactcacc agcaccggat aacctgctat
    19741 ggtccgcgct tcaaaccagg caatgcggtt atccgcatcc accgtttggt cattccagat
    19801 agccgccgta tacaacacgg cgtggttata aatttccgca attgcagcac agtcggcttt
    19861 gcgggcaaaa cggatggaca tgtctggcct cgataaaata atgatgatga tgttatcaat
    19921 actatcgaaa acagcccgaa gcaacggatt cctctatgaa attattgcgt gttgcagagg
    19981 ggatgtgacg gctgcaaaca aaaccaatcc tgtgagcctg ctactattga tggattcatg
    20041 ccaaaagcac aggagagcat tatggggcaa caaacgcagc gtaaccgatg ttgggttctg
    20101 tcctcgcgtc cgcatggcgc gccggttcct tacgtctggt ttcggtaaat gcgctctttg
    20161 tcgcaccagc gcaagcattt acagttccgg aagtgggaaa acgtcaatta aatgaaatca
    20221 atcggatccg ctacgaactg gagttaatgg cagttgctct ggctgtcgaa aacctcacta
    20281 tgcaaaaaaa cgttgctatg ttgtgccagc aatacagccg ataaataaaa tacgcccggc
    20341 aaataccggg cttaaagata attactcaaa tcgccacgca atatttatgc cgacaccata
    20401 atttcgtccg ggcgcaggtt cgtaatatcg cccgtttgac tcattgacaa tgacagaacc
    20461 aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacgg ttaaattgtg
    20521 gtaattgtat ttatacccgg tgaataagcc gacgagtgta taagaaggcg ctttggccgt
    20581 attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg cataccaacc
    20641 atcttccggt atataaccta tcgacgcaaa gcccatatta cgggcgatcc ctggcatccg
    20701 gttaccgtta caatcctgtt cattgcagac attgctgcga taggtcgcat caagccaggt
    20761 ccaggacgct tttacgcgaa aatctcccgc gaaacgttga tcccatgcca gttcagcgcc
    20821 ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac tgctatcgac
    20881 aacaatttca tcatcggtgt cggtctggaa caatgcgaga ctaagcagtc catcaccaat
    20941 acgcgtttta ctgccgatct caattgtatc gttggtggag ggttttaaac caaagttcat
    21001 accgctttgt ccatcagcgc gataagacag ttcattaatc gtcggcgttt caaaaccgcg
    21061 cccggctgcc agatagacat tccaggcatc ggtcattgca tattttaacg atccggcagg
    21121 tagccatttg tgataactgg catcaccgct gtcatcgccg ttacccggag taacgtaatg
    21181 atcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg aaagtttgtc
    21241 ggtcagttgc cactgggttt gtaaataggg atcgacgttc cacatcaaat tgcgttcgtc
    21301 gcggcgcaac tcaccttttt gcccgtattc cggcacgccg cgattcaggc ggaagttatt
    21361 gtaacccttg cggttttcac tcatgttttc gtagttcagg ccggttgtga acgtgactgg
    21421 aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat ggcgttgcag
    21481 ggtaatcacg ccgcccgcat gtgacgggtt aagttgcggg gccatgggga ttgactggta
    21541 ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt gtgcgctcag
    21601 gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgca tgtcgtactg
    21661 ttctgcacga ggcgcttgtt gtggattcgc cttccattct gcttcggtta gcccacctgg
    21721 gtcatctgct ttgatatcca cactattgaa aatcaggctt aatttgctgg cgtcatcaat
    21781 gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt cacgatagcc
    21841 gtgggtcgta aaacgtgtgg ttgcgacggt gtaatcgaca tcgccgggtt gtgtgccgtc
    21901 gcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc cgtagtaact
    21961 actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca ttacaccgcc
    22021 agacgcgttg ccatacagag cagagaaggg gccacgcagc acttccacat tttgcacact
    22081 gcttaaatcg atgttagatg tctgcccttg tccgtcgggc atggtagcgg gaataccgtc
    22141 cacatacagg cgaataccgc gaattccgta agtggagcgg gagccaaatc cgcgaatcga
    22201 cagctgtaaa tcttgcgcat agttctgtcg gttttgtacc tgtaaaccag gcactccggt
    22261 cagtgattca gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc catccaccac
    22321 gctcacggct gctggggtat ccagttctga aaccacctgc ggtgcggcac tgacaatcat
    22381 ggtctgttca tcagcggcaa aaacaacggg ggaaaggaca agcagtgcgg gcaaaacggt
    22441 ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa aacaccgtca
    22501 tggtaataaa attgtaaatt tatggaaaac aaaacggcac aatacgttaa gtaattgaga
    22561 aaattgtagt cataacggta agaaatgctc cacatttgag aaaataatga ttaccattcc
    22621 catttataac aagggcgtaa cgatgattac gcttagcgaa gcattgtgaa gcagcaaaaa
    22681 tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat cgcgcctgcg
    22741 tggttcatta ctgttaggtt cattgctcgt tgcttcatca ttcagtacgc aggccgcaga
    22801 agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata gccagcaaga
    22861 aaacgcgctg tggattgcca cttcgcaaag ccgcaaactg gataaaggcg gcgtggttta
    22921 tcgtcttgat ccggttactc tggaagtgac gcaggcgatc cataacgatc tcaagccgtt
    22981 tggtgccacc atcaataaca cgactcagac gttgtggttt ggtaacaccg taaatagtgc
    23041 ggtcacggcg atagatgcca aaacgggcga agtgaaagga cgtctggtgc tggatgatcg
    23101 taagcgcacg gaagaggtac gcccgttgca accacgtgag ctggtagctg acgataccac
    23161 gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg ttgatggcga
    23221 gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg gtctggcgct
    23281 ggatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga ttaccatcga
    23341 caccaccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca aagagcactt
    23401 ctttatcaac atcagccttg ataccaccaa tcagcgtgca tttatcaccg attctaaagc
    23461 ggcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg ttgcggcacc
    23521 ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtga cgcatcgtca
    23581 ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtggtgaaaa cgttcgatac
    23641 gccgactcat ccgaacagtc tggcgctgtc tgccgatggc aaaacgctgt atgtcagtgt
    23701 gaaacaaaaa tccactaaac agcaggaagc tacccagccg gacgatgtga ttcgtattgc
    23761 gctgtaatag atatggcgag ggtgaaaacg gaaagaattg cctgactttt atcacgccgc
    23821 atccggcacc ttcgtcagta gtctgaattt gcccggcacg tagccgggca attttgcaat
    23881 taactcgccg tttcaggctt aaacacaatg tcctgttttt cttcatcttc ctcgacgact
    23941 ggcgcagtgc tgtgaatttc agcaacgcgt ttgcgcacac caaaccagcc tataaccagc
    24001 agaataccga taatcggcag cgccgcgatg gtataagtcc cgttcgggta atcgaacgcc
    24061 atcagcacca ggacactcag taaaaacagt aatgtcagcc aggaagtgaa gggcgcgcca
    24121 ggcagtttaa aactgacatc cgctgctttg ccttctttaa tcgctttacg caggcgcatc
    24181 tggcaaacga tgataaacgc ccatgaagcg atgattccca gcgacgcgaa gttcaacaca
    24241 atctcaaata cgcgtgacgg caccagatag ttgaggaata cgccgacgac atacacaacc
    24301 agtgtcgcca gaatcccggc atacggcaca tgctgacgac tcattttcgc cataaaactc
    24361 ggtgcggaac cgcccatcgc catcgagcgc agaatacgcc cggtgcagta cagacctgaa
    24421 ttcaggctgg agagggcagc ggtcagcacc acaatgttca taatgctgcc tatatatggc
    24481 acacccagtt tagagaaaaa cgtcacgaac ggactttgcc ccgcctgata tgcgctccac
    24541 ggcaataaca taaccagcaa caccacggag ccgacgtaaa acagaccaat acgccaaatc
    24601 acactgttaa tcgctttagg taccatggtc tgcggatctt tacattctcc ggccgctgta
    24661 cccaccattt caatggaggc aaaagcaaac actacgccct gaatcaacac cagcgcaggc
    24721 agcaaaccgt gggggaagaa gccgccatta tcggtgatta aatgaaagcc agtggtgttg
    24781 ccatccagcg gctgaccgct accgaggaac actgtaccca cgaccaaaaa ggttacaatg
    24841 gcgagcactt taataagcgc aaaccagaac tccatctccg caaaccattt cacaccgatc
    24901 atattcatgg tgccaacgat ggtcagtgca gcgagcgcaa agacccactg cggcacgccg
    24961 ccaaacgcgc cccagtaatg catatacaga gcgacggcgg taatatcaac aatccccgtc
    25021 atcgcccagt tgatgaagta catccagcca gcaacataag cggccttctc acccaaaaac
    25081 tcacgggcat aagagacaaa actgccactg gaagggcggt gtaacaccag ctcacccaat
    25141 gcacgcaaaa taaaaaacga aaacaggccg cagatcaaat agaccagtgc cagtgcgggc
    25201 cccgccattt gcagtcgcgc ccccgcgcct aaaaacaaac ccgtaccaat cgcgccgcca
    25261 atggcgatca tctgtacctg gcgattgccc atcgctttgt gatacccctc ttcgtgggca
    25321 ttaagccagc ggcgtttcgc ggcgtgttga tctgaagtgt cggtgttgtg tttactcatt
    25381 gctctccctg attgctttaa tgaaaaagtc atataagttg ccatgaacaa tgtttattca
    25441 tcctggcgat gacaagattc accattattc tttatgggta taactgtcgg cgcagaatcc
    25501 taccttttat catgtaacga tgcaaaataa tcccgctata aatatgtggg gataaagtcg
    25561 gctggcgaca aaatagcaac ctgatgaaat tatgacggaa acaaccacta ttggtttgat
    25621 gatacctgac tgcagtccta ctcttgcgcc actcgttcgt cgctctgaac gccagtggtt
    25681 gcagtggaca ctggcgtggt tggctcaatg aatagtgagg ggctagcaca tgatgggttt
    25741 caggggtaat acgccaaata ccaatacaat tgctgattaa atgagccaat tgccgacatt
    25801 acagaaaagg ctaaaaaatg taagaggcat gtagggtgat aaacgcgcta ttgcccaaca
    25861 ggagtttctg gaattagtcc tgttccatgg gcattacaac tct
//
LOCUS       DAJOTB010000060        25435 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJOTB010000060 DAJOTB010000000
VERSION     DAJOTB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.060"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(21..1880)
                     /gene="gss"
                     /locus_tag="OHN28_002412"
     CDS             complement(21..1880)
                     /gene="gss"
                     /locus_tag="OHN28_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCQ2297800.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2085..2951
                     /gene="yghU"
                     /locus_tag="OHN28_002413"
     CDS             2085..2951
                     /gene="yghU"
                     /locus_tag="OHN28_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCQ2297801.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADIAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="OHN28_002414"
     CDS             complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="OHN28_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCQ2297802.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="OHN28_002415"
     CDS             complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="OHN28_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2297803.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="OHN28_002416"
     CDS             complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="OHN28_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCQ2297804.1"
                     /translation="MTEEIAGFQTSPKAQVQVAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4169..4663)
                     /locus_tag="OHN28_002417"
     CDS             complement(4169..4663)
                     /locus_tag="OHN28_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCQ2297805.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="OHN28_002418"
     CDS             complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="OHN28_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCQ2297806.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="OHN28_002419"
     CDS             complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="OHN28_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCQ2297807.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="OHN28_002420"
     CDS             complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="OHN28_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCQ2297808.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGGNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="OHN28_002421"
     CDS             complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="OHN28_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCQ2297809.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9827..10114)
                     /locus_tag="OHN28_002422"
     CDS             complement(9827..10114)
                     /locus_tag="OHN28_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCQ2297810.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10233..11120)
                     /locus_tag="OHN28_002423"
     CDS             complement(10233..11120)
                     /locus_tag="OHN28_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCQ2297811.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLDPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGGNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11277..12317
                     /gene="gpr"
                     /locus_tag="OHN28_002424"
     CDS             11277..12317
                     /gene="gpr"
                     /locus_tag="OHN28_002424"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCQ2297812.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12357..12851)
                     /locus_tag="OHN28_002425"
     CDS             complement(12357..12851)
                     /locus_tag="OHN28_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCQ2297813.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13042..13926
                     /gene="yghA"
                     /locus_tag="OHN28_002426"
     CDS             13042..13926
                     /gene="yghA"
                     /locus_tag="OHN28_002426"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2297814.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALMTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13965..14390)
                     /gene="exbD"
                     /locus_tag="OHN28_002427"
     CDS             complement(13965..14390)
                     /gene="exbD"
                     /locus_tag="OHN28_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCQ2297815.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14397..15131)
                     /gene="exbB"
                     /locus_tag="OHN28_002428"
     CDS             complement(14397..15131)
                     /gene="exbB"
                     /locus_tag="OHN28_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2297816.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15383..16570
                     /gene="metC"
                     /locus_tag="OHN28_002429"
     CDS             15383..16570
                     /gene="metC"
                     /locus_tag="OHN28_002429"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCQ2297817.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16710..17369
                     /locus_tag="OHN28_002430"
     CDS             16710..17369
                     /locus_tag="OHN28_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2297818.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17409..18308)
                     /gene="yqhC"
                     /locus_tag="OHN28_002431"
     CDS             complement(17409..18308)
                     /gene="yqhC"
                     /locus_tag="OHN28_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCQ2297819.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIRAILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18502..19665
                     /gene="yqhD"
                     /locus_tag="OHN28_002432"
     CDS             18502..19665
                     /gene="yqhD"
                     /locus_tag="OHN28_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCQ2297820.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARILITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19770..20597
                     /gene="dkgA"
                     /locus_tag="OHN28_002433"
     CDS             19770..20597
                     /gene="dkgA"
                     /locus_tag="OHN28_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCQ2297821.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20797..21723
                     /locus_tag="OHN28_002434"
     CDS             20797..21723
                     /locus_tag="OHN28_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCQ2297822.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     PIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFFRPNSGSLLKQIEAKTAARLKQ"
     gene            21774..22031
                     /gene="yqhH"
                     /locus_tag="OHN28_002435"
     CDS             21774..22031
                     /gene="yqhH"
                     /locus_tag="OHN28_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCQ2297823.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22073..24292)
                     /locus_tag="OHN28_002436"
     CDS             complement(22073..24292)
                     /locus_tag="OHN28_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCQ2297824.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24544..25293
                     /locus_tag="OHN28_002437"
     CDS             24544..25293
                     /locus_tag="OHN28_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCQ2297825.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
BASE COUNT         6352 a         6750 c         6347 g         5986 t
ORIGIN      
        1 cggggttgct acaacatgta ttactctttc accacaatta acggttcaat atcactctct
       61 tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg
      121 gtgaaagtac atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga
      181 tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg
      241 ctgacgaggt cgatgttact gccacagcga ccggcgatcg gtttaacggc ataaccggtt
      301 ttcaccagct catcattcac ggtgaagtca gtatccaaca gataacgatg gtgcgggaac
      361 agagaccaga ggatcggcag aatcgctttg ttaccgggga tcaccgtcca tagcggctca
      421 aaaaccaaca cttccgggcg taacagaacg tctatcagac gaacttcatt ttgtggatgc
      481 ccggtacgga tcggcactgc ggcaaattca cggtcgctca cttcgcggat ttgatcaaat
      541 gccgtttccc acgcccaggt tttccatacg cagttaacca gtcgcccttc cccatcaatc
      601 agttgcccgg cagcatccca gcccagttca tccaacccac gcaagatacg cgtttcaaag
      661 cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg
      721 tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccacgcgcc agccagttcg
      781 ttaatcagtc cttccgcagg attgaagccg ttgcctttat agccctgctc cgcccaacgt
      841 tcgaggatca agcccgcttc ggtatgacag gaggcagaat cggcgttgta ttcgtagact
      901 ttcaggccac gttcatccat gcagaaatcc atacgaccag tgatcatatg atgacggcga
      961 cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcaaa cagcgccagc
     1021 aggttgtcat ctttcagcac cttgtcggtg gcgtgaagat acatcaggtg cagctcgttg
     1081 gtggctttaa tcagctcctg ctcggcactc tcggtaatgg tgtagtaatg atacggatcc
     1141 tgattgatca cctggccgtt ggcctgtaca taggcgtttt gcagcggatc tttttcatcc
     1201 agccatttac cgtcaaactg gcctttgttt tccagacgcg ccccgctgat tttcagcagt
     1261 tcgcctgcaa tttccggctg tggcaagctg tattcggtat cttccgtctg gatcatccag
     1321 cccagaatag tggtgtcatc gaaggtgtct ttcagggtat agccgccgtt ttcgaccacc
     1381 atctccagct cgcgtgtcca ctgttgccct tgcggcaacg gggtatgaat cacgttctgt
     1441 tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct
     1501 ttaaattcac cgcctttatc ccagataaga agcgcccccg ctaccggtgc acgcggcgaa
     1561 ccgttaggaa atgcctgcaa tggcaggatg ttgtcattga ccacttcacg caggaagcgc
     1621 agtgagaaaa tctcccatgc catacccacg tcagtgaaga ctacaccgta gttcagaaag
     1681 agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc atcgtcgata
     1741 tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa
     1801 gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg
     1861 ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa
     1921 ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata
     1981 cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga
     2041 cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt
     2101 atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca
     2161 atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa catccattgc
     2221 aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctgc
     2281 tggcactggg cgttactggt gcagagtacg acgcctggct gattcgcatt ggcgatggcg
     2341 atcaattctc cagcggcttt gtcgaagtga acccgaattc gaagatcccg gcattgcgcg
     2401 atcataccca taatccgccg atccgcgtgt ttgagtcagg ctcgattctg ctttatctgg
     2461 cggagaaatt tggctacttc ttgccgcagg atctggcaaa gcgtactgaa acgatgagct
     2521 ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt cacttttacc
     2581 attacgcgcc ggtgaaaatt gagtacgcca tcaaccgctt tactatggaa gccaagcgcc
     2641 ttctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca
     2701 ccattgcgga tatagcgatt tggccgtggt ttggcaatgt ggtgttaggc ggcgtatatg
     2761 atgccgcaga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg gcgaaagaag
     2821 taggcgaacg tccggcggtg aagcgtgggc gtattgttaa ccgcatcaac ggaccgctga
     2881 acgagcagtt gcatgaacgc catgacgcca gtgatttcga gacaaatacg gaagataagc
     2941 gtcaggggta atggttagcg ttcgtcgcag caagccatcc agaccaatgt aggccggata
     3001 aggcgttcac gccgcatccg gcaatcaggc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcaggccta cgaaagcatc cggctttaat catcacgcgc tggtaatgtc gtaatccatt
     3121 tggcgcagtg cgtctaatgt ggctttggct tcgtcttcat cgataatgct catggcaaat
     3181 ccgacgtgca ccagtaccca ctggcccagc agatcggcag ggttaccttc acaaatcagg
     3241 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
     3301 tcaccgacag ccagcacttg gcctggaacg ccaatacaca ttgttaactc cggttattca
     3361 acttcaatac ttttgacgat tagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga
     3421 cagatcggac actgcgcatc gtgctgatga atctccacca cctggctgca atcccaacac
     3481 caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgctac cgttccgtgg
     3541 cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag tgcaccaatt
     3601 tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt
     3661 tcaacggcgc tctggcaaag cgacaactca tgcattttcg ccactcctgc ggccaaatag
     3721 cagggcacga cgccctgcgt gtggtacatc cggattggtg actggcagcg aaaggatcat
     3781 tcgggcgcag tcatccgtca gccgttggcc ctcttcaatc gacatgctgt gcgaaagcgg
     3841 cgacatcagc gagcaggaga ggtattgcga aacaccgtcc agttcaccaa cagtaaaggt
     3901 catagtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag
     3961 ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt
     4021 ccactgacct tcgaacagcg taaagtcaga aacatacacc ggcattgaag gatgcagaaa
     4081 agagagatca tgcatcgaac gccgggcaat ttcttcaaaa gctacctgta cttgcgcctt
     4141 cggggaggtc tggaaacctg ctatctcttc agtcatgagt cgcctcccgt gggatagctt
     4201 ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca atcatcgctt
     4261 cgaccgtcgg tgtcaaaccg atgtgtggct ccagtgattc cgggatcacg ccgaccaggg
     4321 tcagcttttt cggaaactcg ccggtgaagc gcagggccga caatacgtcg gccaggccaa
     4381 gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca
     4441 tcatcgttcc cggtgcgttc ttttttgaca caatggcatc cgcaataatc agatgatcgc
     4501 gatttgccat gtcaccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat
     4561 aatccggcag aatgtatcgt tgctctaacg cttcaacaat ccgcacaccg atggcttcat
     4621 cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga
     4681 aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa
     4741 ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac
     4801 cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcgggccag agttccaggt
     4861 tgatggaaca accgcctggt agttactgat aataccgtct ttgatcacca tccagtgaga
     4921 gagcataccg cgcggtgctt cgaggaagcc tacgcctttg aactcacccg ttgccggaat
     4981 gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta
     5041 ttgattttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc
     5101 cagcgtggag tgcagctgcg ccacttccag cgtgttgcca gtcagtttct gataaatcgc
     5161 aacgatttca ttcagtttgt tctgggtaga ctcgcgtcct gccgccagtt tcaccagcat
     5221 gttagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga
     5281 atatttaccg tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggtgc
     5341 ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaattc ctttgatcag
     5401 gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta
     5461 gccgcccggg aacaggaagc tgccattttt actgtcggtc gggaattccg gcacgctcag
     5521 gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcag cgataactgc
     5581 agtatcaacc ttgtaaacct gctcaacaaa gtcgctcagc ttgtcgatga aggacttgat
     5641 gtacatcagg cgctcaaggt tcagcacccc cagaccgtcg aggttgatcg ggttcgcgac
     5701 accacctacc gccaggttct gaatatgcgg cgttttaccg cccagcagcg ccacaacgcg
     5761 gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac
     5821 ttccggcggc agtttcattg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg
     5881 accgctggca accagatctt tgatcttgtt ctgaactttg gtgaactctt ccgggctgtt
     5941 cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggctggat cagcttgcag
     6001 tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa caatatggtc
     6061 atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc
     6121 gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac
     6181 gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc
     6241 ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat
     6301 ttcgcaatcg atgcgtaaat gcccttcaat acgggttacc ggatcaatag taattctctg
     6361 gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc
     6421 ggcagtagac gaatgagtac gatataagcg caaatctcaa tagccacaaa accaatagaa
     6481 atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cacccgggtt gaatgccacc
     6541 agcgaatagg tcagacgcca ggttgcacaa cctaacagtg cactcagtgc tgacaggaac
     6601 agcatgcggg aatcattacg caacttcgcc acacgcagaa ccaccagcgg gaagagcatc
     6661 agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc
     6721 ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag caacacactg
     6781 atggtattgg tcagcttaac gaacagactc ttttcatccg gaccgttgcc acgcagaccc
     6841 gcctgcacca acgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc
     6901 agcgagaaca gcggcaacat ttcatagctt tgccacagcg gatgcacctt gtagcccgcc
     6961 gagatcatca gcgaccccat tgaagactgg tgcatagttg gcagcagcgc accaagcgcg
     7021 atgatgaaga acatcacctt gttcagacgt ttgagtgaca ccttccagcc cagacgttca
     7081 aacaatgccg gagcaaactc cagtgccatc acaccgatgt agatggtcat acagaccgcc
     7141 gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc
     7201 cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgaataacc aaacagactc
     7261 gccaacagcg ccggacgcac cagcggatgg tattgcccac gattaaagac gtatactgcc
     7321 cacgccagcg cccagccgcc acaggcaaag ccggtaccga tcagcaggtc aaacgcgatc
     7381 cacacgcccc acgggaagcc gccgttcagg tcggagacag agcccagacc gaacaccaga
     7441 cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta
     7501 ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg
     7561 atggtcgttt ttagtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg
     7621 tagcatcatg cctttataca gggtgtgttg gacatgttcg gaacgcgcac cagtagaaag
     7681 atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg
     7741 agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag
     7801 gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc ccggcttcag
     7861 cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccaaaaa tcaccgcgcc
     7921 cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt
     7981 ctggttgcac aactcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg
     8041 cacgttgtat gggcaggcga ccatgcagta acggcagccg gtgcacacat ctttgtcgta
     8101 atggacaatg ccggttttcg gatctttttt cagcgcagag accgggcaca cagagacaca
     8161 gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccat tctcctcctg
     8221 gtctttgttg ccccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga
     8281 cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat
     8341 atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat
     8401 ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag
     8461 cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt
     8521 tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc
     8581 atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgcc
     8641 gagacgttgc cgccttcttt agcgttaacg tccggtttct gtgaacgcgg agtctggttt
     8701 tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca
     8761 tagcacgggt gaccaatcgc taccggccac acgccgccaa catcgcagaa ttgcagcgtt
     8821 gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccaaccttcg
     8881 cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt
     8941 tcacagtgtt cgtgaatcag acgaccatag gcgaaagtcg gacggttttt gtcatccagt
     9001 ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc
     9061 ggcgggcagc caggaatatt gataacggtt ttgcccggta gaacttcttg caggctgact
     9121 gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca ggaaccgata
     9181 gcgataatgg ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg
     9241 gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca
     9301 tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag
     9361 gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagcacgag gttttctacc
     9421 gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc
     9481 cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg
     9541 ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta
     9601 atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag
     9661 acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc
     9721 acacatttat tatggttatc gatatcatga tactgcggcg gggcactaaa acgaagggaa
     9781 gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttctaggc
     9841 gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca
     9901 ttaccatctc tttatccagt ccataacggc gcgtcagaat acccgcttcc caggcgctaa
     9961 gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt
    10021 tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc
    10081 ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgc tatatattgt
    10141 catatatagc gatattttag gcaaagaacg acatttgcgc acgctatttt ttcatgcttg
    10201 tttaagacgt aatcctgtaa gagataaacc catcaggaga gatatttatc gaaccatttc
    10261 agcgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta
    10321 tggaatccgt gattaccccc cggatatata tacgcctcat agaccttatt attggctttc
    10381 aacgccgcct cgtaagcggg ccagccctcg ttgattcggc tatccagttc agcgtagtgg
    10441 agcagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg cccataaaac
    10501 ggcaccgcgc aggccagttc cggatacgcg actgctgccg cgttcgatac gcccccgcca
    10561 tagcaaaatc cgctaatacc cactttgcct gtcgcttgcg gatagcgttg cataaactca
    10621 attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga
    10681 cctttatcat catttcccgg ataacctcca acggaactta agccgtcagg tgccagggcg
    10741 atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatc cagtccacga
    10801 ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg cttcaccaga
    10861 taaccccgta cctcgccgtg accatttggc gagggatacg tgatgtactc tgcaacaatt
    10921 tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac
    10981 agcgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag gaactcacgt
    11041 ttcgagattt tcccgtgagc gtaatagtcg tagtaatcca gcaactcttg tgggaaatct
    11101 ttggcggtta aacgcggcat cgttgcactc ctcaactggt gtttttttaa gcaaagcata
    11161 agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg
    11221 gcgaatcgct aaactagaaa cattgtttcg aaattgaacg atggaaagga gaggtcatgg
    11281 tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgt ggaaaaagtg
    11341 gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc
    11401 tggaatcaca gcgtgcgatc ctgcgtaaag cgtttgattt aggcattacg cactttgatt
    11461 tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc
    11521 gcgaggattt tgccgcttat cgcgatgaac tgattatctc taccaaagcc ggatacgata
    11581 tgtggcccgg cccgtacggc tctggcggct cacgtaaata cctgctcgcc agcctcgacc
    11641 aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg
    11701 aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg
    11761 cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg gttgagttgc
    11821 tgcacgagtg gaaaattccg ctgttaattc atcaaccttc gtacaattta ctgaaccgct
    11881 gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgcattgcct
    11941 ttactccact ggctcaggga ttgctgaccg gaaaatatct caacgctatt ccgcaagatt
    12001 cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg ctcaccgaag
    12061 ccaacctcaa cagcctgcgc ttattgcatg aaatggcaca gcagcgtgga caatcaatgg
    12121 cgcaaatggc gttaagctgg ttgctgaaag atgagcgagt gacgtcggta ttgattggag
    12181 ccagccgcgc ggagcaactt gaggagaacg tgcaggcgct gaataatctg acatttagca
    12241 ccgaggagct ggcgcagatt gatcagcata tcgccgatgg agagctgaat ctgtggcagg
    12301 cgtcttccga taaatgacct gttaataacg ggcgcgagaa ccgcgcccga atcagatcag
    12361 tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagagag cacaaacgtc
    12421 agatggataa tgacgtacca catcagtttg ttatcaggga catttttcgc atccataaag
    12481 acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc
    12541 gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcaga gatatcgagt
    12601 tgtgagacga aattctcgta accggaaaac atcaccatca ccagcaaacc gccaaccagc
    12661 gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata
    12721 ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc
    12781 gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga
    12841 aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattaagt
    12901 aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata attctgaggg
    12961 ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt
    13021 gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca gtattacact
    13081 ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccggta
    13141 ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagcactg
    13201 atgacagggg gcgattccgg aatcggtcgc gctgccgcca ttgcttacgc gcgtgaaggg
    13261 gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag
    13321 atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa
    13381 ttcgcccgtt cgctggttca cgaagcacac aaggcgttag gcgggctgga tattatggcg
    13441 ctggtagccg ggaaacaggt tgccattcct gatattgcag acctcaccag cgaacagttt
    13501 caaaagacct ttgccattaa cgttttcgcg ttgttctggc taacccagga agcgatcccc
    13561 ctgctaccga aaggtgcaag tatcatcacc acttcctcaa tccaggcata ccagccaagc
    13621 ccgcatttac tggactatgc ggctacgaag gcggcaattc tgaactacag ccgtggcctg
    13681 gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg cccgatctgg
    13741 acagcactgc aaatttctgg cggacaaacg caggataaga tcccgcagtt tggtcagaaa
    13801 acgccgatga agcgtgcggg gcaaccggcg gagctggccc ctgtatatgt ttacctggca
    13861 agtcaggagt cgagctacgt caccgcagaa gtgcacggtg tctgcggcgg cgagcattta
    13921 ggttaaaaaa atgcccggtt gtgaaaagta accgggcatc atttttattt cgctttcgca
    13981 gtttcttcgc cgaccagacc tattttcagg taacccgcct gatgcagcgt atccattacc
    14041 ttcatcaacg tctcgtaatc gacagtttta tccgcacgga agaagatggt ggtgtctttc
    14101 ttgccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac cggatcgtta
    14161 ccaataaaca tcgagttgtc tgctttcact gacagataaa ccggtttttc cgggcgcggc
    14221 tgcggcgtgc tggtagaagc aggcaaattc accttcacat ctaccgtcgc taacggtgcc
    14281 gccaccataa agataatcag caaaaccaac atcacgtcga taaacggcgt cacgttgata
    14341 tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcatcgccat cggacattat
    14401 cctgcacgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc ttccagatcc
    14461 aggtcacggc tttgcagcaa caatacctgc gctgcaacat cacccagcat cgctttaaag
    14521 ccgccaatct ggcgtgcaaa tacgttatag ataacgaccg caggaatcgc tgccacgagg
    14581 ccgattgccg ttgctaacag agcttctgca atacccggcg caacgactgc caggttagtg
    14641 gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt accaaacaga
    14701 ccaacgaacg gcgaaatcgc gccgatggtt gccagatagc cgttaccgcg gcccatttga
    14761 cgacctactg cggcgacccg acgctccaga cggaagctgg tacgttcttt aataccttcg
    14821 ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag caaatgcagg
    14881 cttaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa ggaacgcgct
    14941 tcagccagca gttgctgctc gcgcttaagg cgacgcttct gattgaagaa ctctacgctc
    15001 ttactgaaga agattgccca ggtgactacg gaggccaaaa taagcccaat catcacgcac
    15061 ttaacgacaa tatcggcgtg ctgatacata ccccagacgg aaaggtccgt ctgcattaaa
    15121 ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgtgcata ataatatcaa
    15181 aacgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta cctttgcttt
    15241 cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga aaattttctg
    15301 ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag acatccagac
    15361 gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac tggtgaatgc
    15421 aggacgcagc aaaaaataca cgctcggtgc ggtaaatagc gtgattcagc gcgcgtcttc
    15481 gctggtcttt gacagtgtag aagccaaaaa acacgcgaca cgtaatcgcg ccaatggcga
    15541 gttgttctat ggacggcgtg gaacgttaac ccatttctcc ttacaacaag cgatgtgtga
    15601 actggaaggt ggcgcaggct gtgtgctatt tccctgcggg gcggcagcgg ttgctaattc
    15661 cattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg cctatgaacc
    15721 gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat cgtggtttga
    15781 tccgctgatt ggtgccgata tcgttaagca tctgcagcca aacactaaaa tcgtgtttct
    15841 ggaatcgcca ggttccatca ccatggaagt ccacgatgtt ccggcgattg ttgccgccgt
    15901 acgcagtgtg gtcccggatg ccatcattat gatcgacaac acctgggcag ccggtgtgct
    15961 gtttaaggcg ctggattttg gcatcgatgt ttctattcag gccgccacca aatatctggt
    16021 tgggcattca gatgcgatga ttggcactgc ggtgtgcaat gcccgttgct gggagcagct
    16081 gcgagagaat gcctatctga tgggccagat ggtcgatgcc gataccgcct atataaccag
    16141 ccgtggcctg cgcacattag gtgtgcgttt gcgccaacat catgaaagca gtctgaaagt
    16201 ggctgaatgg ctggcagaac atccccaagt agcgcgagtt aaccaccctg ctctgcctgg
    16261 cagtaaaggc cacgaattct ggaaacgaga ctttacaggc agcagcgggc tattttcctt
    16321 tgtgcttaag aaaaaactca atgatgaaga gctggcgaac tatctggata acttcagttt
    16381 attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcaa atcaaccaga
    16441 acatatcgcc gccattcgcc cacaaggcaa gatcgatttt agcgggacct tgattcgcct
    16501 gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg gttttgcgcg
    16561 aattgtataa cattgccact tttggacaat tttgcagaca ttttattgtg aaaagtctta
    16621 aattgttgcg tccgggatca aggcgtcccg gacgattcag gagtacaata ggcagataaa
    16681 ggcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc atcgctgcgc
    16741 tctggcaaca cgactttgcc gcgctggcgg accctcatat tgttagcgtt gtttactttg
    16801 ttatgtttgc cacgctgttt ttagaaaacg gcttgctgcc cgcctcattt ttgccaggcg
    16861 acagcttgtt gatactggca ggcgcattga ttgcccaggg ggttatggat tttctgccta
    16921 cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat attcaggggc
    16981 gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct gctaaatatc
    17041 accagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg gctggacgtt
    17101 ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt ctgccaaacc
    17161 gccgcttcca gtttttcaac tggttaagcg gattgctgtg ggtcagcgtg gtaaccagtt
    17221 tcggctatgc cttaagtatg attccgttcg ttaaacgaca tgaagatcag gtaatgacgt
    17281 tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttgggcacg ctgtttgtgg
    17341 tgattaaaaa aaaatactgt aacgcctgac gattttcccc gttcccggtt gctgtaccgg
    17401 gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc cggcgtcaca
    17461 ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc
    17521 gccgccgcgc tggccttcat gccgtcgtgg atgatcatca tccgcgcctt atgcagacgg
    17581 taattcttca aatactgcaa cggcgaggtg ctggtgacag acttaaaatt atggtggaac
    17641 gccgatacgc tcatgttggc ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat
    17701 ttattctcaa tccgtttcag cacgcgacta atcaggctga agtgagtctg gcggctgacc
    17761 agtgccagta acgcgccgcc gcaaggtccg gtcagcacgt agtacagaat tgcgcggata
    17821 atctgtttgc cgagaatgcg cgcatccagc ggtcgctcca tcacatccag taaccgctct
    17881 gccgcgcata aaatctcttc tgataacgtg gcggagttaa taccgctggc tgccattgac
    17941 ggctgaaaat gttcatcttc gccaatgtcc atcaacagtt cctgtaactg caaaatatcg
    18001 acattgagac gcaatcctgc cagcggcacc tctgacgtgg cataggtttc gcactcaaac
    18061 ggcaacggca ccgtcagcag gagatattca ttagcgtcat aacgaaacac gcgttcattg
    18121 atataaccga ttttatgtcc ggaaaagaga attatgatgc caggctcgta catcaccggt
    18181 gtgcgtgcga aaggcgtctc gccatacaac aaacgcacat cgggcaacag ctctgacaaa
    18241 ctattttctt tatttttcag tttattaact ttatccgcca gcaagcggca aatctcttca
    18301 cgtttcatat cgcgtaattt cttaggaata atgcggcaat ttgattgtgc gcaattttgt
    18361 agcatttctc cagcactctg gagaaatagg caagacattg gcagaaatga gcattgagag
    18421 gtacggcgct ggcgaccaca atgaaaaaca tcaggcagac cgatctctgc cctcatattg
    18481 acccagcaaa gggagcaagt aatgaacaac tttaatctgc acaccccaac ccgcattctg
    18541 tttggtaaag gcgcaatcgc tggtttacgc gaacaaattc ctcacgatgc tcgcatattg
    18601 attacctacg gcggcggcag cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc
    18661 ctgaaaggca tggacgtgct ggaatttggc ggtattgaac caaacccggc ttatgaaacg
    18721 ctgatgaacg ccgtgaaact ggttcgcgaa caaaaagtga cttttctgct ggcggttggc
    18781 ggcggttctg tactggacgg caccaaattt atcgccgcag cggctaacta tccggaaaat
    18841 atcgatccgt ggcacattct gcaaacgggc ggcaaagaga ttaaaagcgc catcccgatg
    18901 ggctgtgtgc tgacgctgcc agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc
    18961 cgtaaaacca caggcgacaa gcaggcgttc cattctgccc atgttcagcc cgtatttgcc
    19021 gtgctcgatc cggtttatac ctacaccctg ccgtcgcgtc aggtggctaa cggcgtagtg
    19081 gacgcctttg tacacaccgt ggaacagtat gttaccaaac cggttgatgc caaaattcag
    19141 gaccgtttcg cagaaggcat tttgctgacg ctgatcgaag atggtccgaa agccctgaaa
    19201 gagccagaaa actacgatgt gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac
    19261 ggtttgatcg gcgctggcgt accgcaggac tgggcaacgc atatgctggg ccacgaactg
    19321 actgcgatgc acggtctgga tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg
    19381 aatgaaaaac gcgataccaa gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac
    19441 atcactgaag gttcagacga tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc
    19501 tttgagcaat taggcgtgcc gacccacctc tccgactacg gtctggacgg cagctccatc
    19561 ccggctttgc tgaaaaaact ggaagagcac ggcatgaccc aactgggcga aaatcatgac
    19621 attacgctgg atgtcagccg ccgtatatac gaagccgccc gctaagcttt ttacgcctca
    19681 aactttcgtt ttcgggcatt tcgtccagac ttaagttcac aacacctcac cggagcctgc
    19741 tccggtgagt tcatataaag gaggaacgta tggctaatcc aaccgttatt aagctacagg
    19801 atggcaatgt catgccccag ctgggactgg gcgtctggca agcaagtaat gaggaagtaa
    19861 tcaccgccat tcaaaaagcg ttagaagtgg gttatcgctc gattgatacc gccgcggcct
    19921 acaagaacga agaaggtgtc ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac
    19981 tgttcatcac cactaagctg tggaacgacg accacaagcg cccccgcgaa gccctgctcg
    20041 acagcctgaa aaaactccag cttgattata tcgacctcta cttaatgcac tggcccgttc
    20101 ccgctatcga ccattatgtc gaagcatgga aaggcatgat cgaattgcaa aaagagggat
    20161 taatcaaaag tatcggcgtg tgcaacttcc agatccatca cctgcaacgc ctgattgatg
    20221 aaactggcgt gacgcctgtg ataaaccaga tcgaacttca cccgctgatg caacaacgcc
    20281 agctacatgc ctggaacgcg acacacaaaa tccagaccga atcctggagc ccattagcgc
    20341 aaggagggaa aggcgttttc gatcagaaag tcattcgcga tctggcagat aaatacggca
    20401 aaacacctgc gcaaattgtt atccgctggc atctggatag cggcctggtg gtgatcccaa
    20461 aatcggtcac tccttcacgc attgtcgaaa actttgatgt ctgggatttc cgtctcgaca
    20521 aagacgaact tggcgaaatt gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg
    20581 accagttcgg cggctaacgt gcaaattctc ccggtggcgg taatgttccg ctaccggact
    20641 tttcagaaat catttattcc cctcgcgtcc cgcccgttgc tactcttcct tgttcaggaa
    20701 tgccaaatat aaggacatca tcatgcagag ccggaagctc ttaaaagaac aactcatcta
    20761 tatccgggat aaacgcaacg gagaggtgaa aaacagatga aaataatact tctgttttta
    20821 gcagccctgg caagttttac cgtacacgca cagcccccct cacagaccgt agaacaaaca
    20881 gtccggcata tttatcagaa ctataaatca gatgccactg ccccttattt tggtgaaacc
    20941 ggagagcggg cgataacatc tgcgcgtatt caacaggcgc ttaccctgaa cgacaatctt
    21001 acgctgccgg gcaatattgg ctggctggat tatgatccgg tttgtgattg tcaggatttt
    21061 ggcgatctgg tgttagaaag cgtagcgata acccaaactg acgccgatca tgccgatgcc
    21121 attgtgcgct ttcgtatctt tcaagacgat aaagaaaaga cctcgcagac actgaaaatg
    21181 gtggcggaaa atggtcgttg ggtcattgac gatattgtca gcaatcatgg cagcgtctta
    21241 caagcggtta atagcgagaa tgaaaaaaca ctggccgcta tagcttcgtt gcaaaaagaa
    21301 cagccggaag cctttgttgc cgaactcttt gaacatattg ctgattatag ctggccgtgg
    21361 acgtgggtgg tttccgactc ttaccgccag gcggtcaatg ccttctataa aaccaccttc
    21421 aagacggcca acaatcccga tgaagatatg ccaatagaac ggcaatttat ttacgacaat
    21481 ccgatctgtt ttggcgaaga gtcgttattt tcacgcgttg atgaaattcg agtcctggag
    21541 aaaaccaccg attccgcccg cattcatgtt cgttttacgc tgaccaatgg caacaacgaa
    21601 gagcaagaac tgattttaca gcggcgcgaa ggtaagtggg aaatcgctga ttttttccgc
    21661 ccgaacagcg gcagcctact taagcagatt gaggcaaaaa ccgccgccag attaaaacaa
    21721 tgagctgaat taaataacaa ttagccggaa caataaataa aagggaacac tatatgaaaa
    21781 cgattttcac cgtgggagct gttgttctgg caacctgctt gctcagtggc tgcgtcaatg
    21841 agcaaaaggt caatcagctg gcgagcaatg tgcaaacatt aaatgccaaa atcgcccgtc
    21901 ttgagcagga tatgaaagca ttacgcccac aaatctatgc tgccaaatcc gaagctaaca
    21961 gagccaatac gcgtcttgat gcacaggact atttcgactg cctgcgctgc ttgcgtatgt
    22021 acgcagaatg ataaaaaaat ccccggcagc attctgttac cggggatatt ttttaacgtc
    22081 caaccgccgc tttagggcgt ttcttcgcac cagcattctg caggcgagat tgcgtagacg
    22141 acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta tgtttagtca
    22201 acgccggacg ggtattgcgg ttctgacggc gagcttcacg catctcttca atggttggcg
    22261 caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc gcttccagcg
    22321 cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct ttatgcaaac
    22381 gacgctgctt gtcgcccttc ggtacgaaga cgtcttcact cttataacca atcttcgcca
    22441 gcgggttttt gccggtgtaa tacatggtgg tcgagttagc cagcggcgac ggatagaagt
    22501 tctgcacctg gtcgaggcgg aagcgatgct ttttcagcca tagcgccaga ttcaccatat
    22561 cttcatcacg cgtacccggg tgcgcggaga tgaaatacgg gatcagatac tgttctttac
    22621 ctgcctgttt cgagtaagta tcgaacagct ctttaaagcg gtcatagctg cccatgcccg
    22681 gcttcatcat cttcgataac ggtccttctt cggtatgttc cggggcaatc ttcagataac
    22741 cgccgacgtg atgggtcgcc agctctttga tatagcgcgg atcttctacg gcgatgtcat
    22801 aacgcacacc agaggcaatc aggatctttt taatgccttt cagatcgcgc gcacggcgat
    22861 agaggttgat cgtcggttcg tggttagtgt ccatatgcgg acaaatatcc ggataaacgc
    22921 acgacaaacg acggcaggtt tgctcagcgc gtggcgattt gcagcgcaac atatacatgt
    22981 tggcagtagg cccaccgaga tcggaaatca cgcccgtaaa acctggaaca gtgtcgcgga
    23041 tcgcttcgat ctcattaata atcgaatctt cggaacggct ctgaataatg cgcccttcgt
    23101 gctcggtaat agaacagaaa gagcagccgc caaagcagcc acgcataatg ttgaccgaga
    23161 aacgaatcat ttcgtaagcc ggaattcggg cattaccata agccggatgc ggcacgcgct
    23221 tgtacggcag cgcaaagacg ctatccatct cttcggtaga aagcggaata gcaggcgggt
    23281 tgatccatac atagcggtcg ccatgttttt gcatcaatgc gcgggcgcag cctgggttag
    23341 tttcgtggtg cagaatgcgc gaagcatggg cgtacagcac tttatcgccc ttcactttct
    23401 cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgtggt ggctgcacgg
    23461 ttacagcttt ggcttcctgc tttttcggag ctaccggttt gttatccgcg cacggcaaat
    23521 cttcgccata cggatgcggg attgggtcga ttttccctgg ggtatcaaga cgggtggaat
    23581 ccacaccgct ccagcctggc aacgcctctt ttacgataat cgcggtatta cgcacatcgc
    23641 ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacttccacc agcggacgct
    23701 caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa cggcgcacgg
    23761 tatccgacca gtaatcataa tgcgcggtac ggcgcaggct ggcctcaata ccgccgagga
    23821 tcaccggcac atctttccac gcctctttac aacgctgggt ataaaccagt gtggcgcgat
    23881 ccggacgctt acccgcgacg ttatccggtg tgtaggcatc gtcatgacgt aaacggcgat
    23941 cggcggtata gcggttgatc atcgagtcca tgttgccagc agtaacaccg aaaaacagat
    24001 tcggtttacc cagacgcata aagtcgtctt tactgctcca gtctggctgg gcgatgatcc
    24061 cgacgcgaaa gccctgcgct tccagcatac gaccgcaaat cgccatcccg aagcttgggt
    24121 gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat
    24181 ccatctcttc acgtgacatc ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact
    24241 gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc attttgcttc
    24301 cagaaatggt aaaaaaataa tcaaaggccg gggattatac gctgtatcac gcgcggaaat
    24361 gaagtgatgt tgatgaacgg tggcgaaata ccctggtgag gtttttcact gcccgacttg
    24421 ggcaagcaca tttaaggctg gcggtatcgt ggcaactgcc gctactattt atgccgctca
    24481 gcaacgttca aatcatattg cacgtcgatc ggctatccga ggacacccac agtaaggcaa
    24541 ggtatggaac aggttattac aaagcgccgc tattacgata ttggtctaca gattgaagag
    24601 ctgctgtatt caggcgtatt taaggccgga gaacgcttac cttcagaacg tgaactgagc
    24661 gagcggttta ataccagccg aactaccatt cgtgaagcca tcattatgct ggaactgaaa
    24721 ggcgtattga atgtgaagca agggtcaggg attttctttg tcgactccac cgataagctc
    24781 aaccagaaat cattaatgcc ctattcagaa attggcccgt ttgaactgtt acaggcacgt
    24841 caggtaattg agagcaatat taccggtttt gctgccagcc agatcagctt taacgagtta
    24901 caagagttga agaaaatcat tggattacag gaaaaagcca tcgctgcgga aagtgataaa
    24961 tttgaagacc tggatcaccg atttcatagc attattgccg aagcgacgca aaatcgggta
    25021 ctcatcaaac aagctgctga attatggcgg gcagttcgta cagaaaaccc ccgctggaag
    25081 aaacttaact acaagtacct gcatgaaaag catttgcgtc tgcaatggct ggaagatcac
    25141 cgggctattt tcctggctct gcaacagaaa gattcagagc tcgccagaga agcgtcctgg
    25201 agacatctgg aaaacagcaa aaatgaactg atcaaaatct tcaaacaaga cgcttccatc
    25261 agcgattttg atgacttttt cttcgctcgt taaccttcat tagtccagag ctggcgggaa
    25321 aaataacgcc ggctctgcct tccgccctcc actggtacac gctctctttt caattggtct
    25381 aataaatcga ctatcatcag atatcttaga ttagcggcaa ttatcccaaa aactg
//
LOCUS       DAJOTB010000061        25145 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJOTB010000061 DAJOTB010000000
VERSION     DAJOTB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.061"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            241..1539
                     /gene="kgtP"
                     /locus_tag="OHN28_002438"
     CDS             241..1539
                     /gene="kgtP"
                     /locus_tag="OHN28_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCQ2297826.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1536..1859)
                     /locus_tag="OHN28_002439"
     CDS             complement(1536..1859)
                     /locus_tag="OHN28_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCQ2297827.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="OHN28_002440"
     CDS             complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="OHN28_002440"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCQ2297828.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3374..6034)
                     /gene="pat"
                     /locus_tag="OHN28_002441"
     CDS             complement(3374..6034)
                     /gene="pat"
                     /locus_tag="OHN28_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCQ2297829.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6066..6764)
                     /gene="tapT"
                     /locus_tag="OHN28_002442"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6066..6764)
                     /gene="tapT"
                     /locus_tag="OHN28_002442"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCQ2297830.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="OHN28_002443"
     CDS             complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="OHN28_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCQ2297831.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7459..8496
                     /locus_tag="OHN28_002444"
     CDS             7459..8496
                     /locus_tag="OHN28_002444"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2297832.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8544..9233)
                     /gene="ung"
                     /locus_tag="OHN28_002445"
     CDS             complement(8544..9233)
                     /gene="ung"
                     /locus_tag="OHN28_002445"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCQ2297833.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9538..9921
                     /gene="grcA"
                     /locus_tag="OHN28_002446"
     CDS             9538..9921
                     /gene="grcA"
                     /locus_tag="OHN28_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCQ2297834.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9977..10564)
                     /gene="eamB"
                     /locus_tag="OHN28_002447"
     CDS             complement(9977..10564)
                     /gene="eamB"
                     /locus_tag="OHN28_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCQ2297835.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10667..11548
                     /locus_tag="OHN28_002448"
     CDS             10667..11548
                     /locus_tag="OHN28_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2297836.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11581..12915)
                     /gene="srmB"
                     /locus_tag="OHN28_002449"
     CDS             complement(11581..12915)
                     /gene="srmB"
                     /locus_tag="OHN28_002449"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCQ2297837.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13047..13784
                     /gene="trmN"
                     /locus_tag="OHN28_002450"
     CDS             13047..13784
                     /gene="trmN"
                     /locus_tag="OHN28_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCQ2297838.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13769..15391)
                     /gene="nadB"
                     /locus_tag="OHN28_002451"
     CDS             complement(13769..15391)
                     /gene="nadB"
                     /locus_tag="OHN28_002451"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCQ2297839.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            15647..15802
                     /gene="rseD"
                     /locus_tag="OHN28_002452"
     CDS             15647..15802
                     /gene="rseD"
                     /locus_tag="OHN28_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCQ2297840.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15799..16374
                     /gene="rpoE"
                     /locus_tag="OHN28_002453"
     CDS             15799..16374
                     /gene="rpoE"
                     /locus_tag="OHN28_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCQ2297841.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16407..17057
                     /gene="rseA"
                     /locus_tag="OHN28_002454"
     CDS             16407..17057
                     /gene="rseA"
                     /locus_tag="OHN28_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCQ2297842.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17057..18013
                     /gene="rseB"
                     /locus_tag="OHN28_002455"
     CDS             17057..18013
                     /gene="rseB"
                     /locus_tag="OHN28_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCQ2297843.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            18010..18489
                     /gene="rseC"
                     /locus_tag="OHN28_002456"
     CDS             18010..18489
                     /gene="rseC"
                     /locus_tag="OHN28_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCQ2297844.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18687..20486
                     /gene="lepA"
                     /locus_tag="OHN28_002457"
     CDS             18687..20486
                     /gene="lepA"
                     /locus_tag="OHN28_002457"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCQ2297845.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20502..21476
                     /gene="lepB"
                     /locus_tag="OHN28_002458"
     CDS             20502..21476
                     /gene="lepB"
                     /locus_tag="OHN28_002458"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCQ2297846.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21748..22428
                     /gene="rnc"
                     /locus_tag="OHN28_002459"
     CDS             21748..22428
                     /gene="rnc"
                     /locus_tag="OHN28_002459"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCQ2297847.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22425..23330
                     /gene="era"
                     /locus_tag="OHN28_002460"
     CDS             22425..23330
                     /gene="era"
                     /locus_tag="OHN28_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCQ2297848.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23342..24070
                     /gene="recO"
                     /locus_tag="OHN28_002461"
     CDS             23342..24070
                     /gene="recO"
                     /locus_tag="OHN28_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCQ2297849.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24082..24813
                     /gene="pdxJ"
                     /locus_tag="OHN28_002462"
     CDS             24082..24813
                     /gene="pdxJ"
                     /locus_tag="OHN28_002462"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCQ2297850.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24813..>25145
                     /locus_tag="OHN28_002463"
     CDS             24813..>25145
                     /locus_tag="OHN28_002463"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCQ2297851.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTL"
BASE COUNT         6160 a         6270 c         6647 g         6068 t
ORIGIN      
        1 ttatactccc acataagcca gattcaacag cgaatacgtc ttccccaaat tgcccacttc
       61 catacttcct ccttaccaga aatctatcct taagctcctt aataaccatt ttcctgctaa
      121 ctaaattcat ggttaaggtt gtataatgat gcgcaacaaa tgtataacat tttctttacc
      181 aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag acataatggc
      241 atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac tcgtcgccgc
      301 atttgggcaa ttgttggggc ctcttcaggt aatctggtcg agtggttcga tttctatgtc
      361 tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa cacgacgact
      421 caactactgc aaacagcagg tgtttttgct gcaggatttc tgatgcgccc aataggcggt
      481 tggctgtttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg
      541 tgtatgatgt gtttcggctc actggttatc gcctgtttgc caggctacga aacaatcggt
      601 acctgggctc cggcattatt gcttctcgcg cgtttattcc agggattatc cgttggcgga
      661 gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg caaaggtttt
      721 tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct actggttgtc
      781 gtggttttac aacacaccat ggaagacgct gtactcagag agtggggatg gcgtattcct
      841 ttcgcgttag gagctgtgtt agctgttgtg gcgttgcggt tacgtcgtca gttagatgaa
      901 acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg attatggcgc
      961 aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct ttgtttctat
     1021 accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca tgccaacgtg
     1081 gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc aattattggc
     1141 gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc tctggcaacc
     1201 atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta tgccgctttt
     1261 ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag tggaatactg
     1321 aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc atatgcgttc
     1381 gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa atcaatagga
     1441 atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt tctggtttca
     1501 ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag ttgccagacg
     1561 gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct ccagccgctc
     1621 ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga gaacatcaat
     1681 ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc atttccggcg
     1741 gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct ccagttatcg
     1801 ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag aatacgcaaa
     1861 acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag gatgcggcta
     1921 attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac cttcaccgga
     1981 taatcggcaa tactttgcag atcgcggtag tgcttaacga tggtggtatg ctcgcggatc
     2041 agatccagtt ctttctctcg ctgtggcgca agctccagtt gcggatcgtg gatcaaaatg
     2101 gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt gatcaaaatc
     2161 cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc tttccataac
     2221 cgaactacta actggtcagt gttgacgtaa tactgtaaac gactcaggaa acgacgcaga
     2281 ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc ttccggaatg
     2341 tagaagtcat tcgcggtttt atcaccaaca ataatttcga cctttttccc ttcgcgcagc
     2401 aactggataa tattgcgcac aaggattgct ggtaggttga agtaaggtgt gcagatggtt
     2461 agtttctgct cggcacaagg cataagatgg aaaatagtct tgttcaacag actcgatttc
     2521 cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga
     2581 taagcggcat cacgcagctc ctggcggaac aggcgaatgt cgttcttgat ttccgggctt
     2641 tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat attctgtgta
     2701 acccattcaa acataatgtc tgacatctta cggttacgga tcagatgata acggtcgtag
     2761 cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct ataaagtacg
     2821 ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg ggtattgatt
     2881 ggaacgccat aaaccggaac atttacgccc ggattttcct gcgccatgcg gcagtaccag
     2941 tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg
     3001 accagcaccc gcacatccag ttccggacgc tgccttttag cctcatacaa cgcgttcaga
     3061 atgcctttgc caccgtcatc ctgttcgaga tacagggcaa caatgcaaat gcgctgcttc
     3121 gcgctggcta ttttttccag caacgtctcc cggaagtcgg cgggagcgta aaagaaatcg
     3181 acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg atgtttatta
     3241 cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag ggtcctctgt
     3301 gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt ggtcaacatt
     3361 tccagtacct tactcatgat tcctcgcgct gggcaagact tagcgtaagc ccaacgatcc
     3421 cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggccagcgcc accatgccac
     3481 gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc gtataggtaa
     3541 tcaacttttc cattaagcgt cgacctaagc ctaacccttt aagatccgag cgaaccagta
     3601 cggcaaattc ggcatcgatg ttatcagggt cggagatcgc acgtgtgacg ccgaggatct
     3661 cttccgtttg atcaattcgt cgtaccgcta caaatgccat ttcccgatcg tagtcgatct
     3721 gcgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag tagcggtaat
     3781 aaagatcttc tttggtgact cgcgaaatga attgctgaag ctgtggctca tcttctggca
     3841 aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct tccagctgat
     3901 gcggataagg gcgcacagcc agtcggctct cgttatcacc ttcaaacggc gcgatatcca
     3961 gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga atatccagac
     4021 gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc tggctcaagc
     4081 ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt ttgatcccct
     4141 gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg acggcttgat
     4201 cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg aaaaccggat
     4261 cgtgctcaac cacaacccgc aactcctgag cgccagcacg gttagccata ctttgcacca
     4321 acaggccgtg gacccgcgcc tgcggccagg ccatttttac gcgatcgaaa atagcgtttg
     4381 ccgcttgctg gacttcattg gctgtacgta ggtagagcat gacgccctga acttccgatt
     4441 tatgtggaat atccggcgag cgcaatttca gtgccaccgg atagccaatc tgttcggcaa
     4501 tatgtaccgc ttcggtgcta tcactggcaa tccaggtagg gagagtattc ataccatacg
     4561 cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg gcaatcgcct
     4621 gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc aacgccggcg
     4681 tttcgcgtag ttgcttctga ttacgccggt actcgaccat atgcataaaa gcagtgatgg
     4741 ttccttccgg agtacggtag gtcggcagcc cggcttcgct gaataaacgt cgtgcctctt
     4801 gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg cggggatgat
     4861 gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg
     4921 aatgaataac catcagcgca tcaaaatcct ggctgtggag cagaatgtcc agcgttttga
     4981 cataatgctc gctgctggcg tcatcgcgca gatcgagcgg gttagagatt gccacatgcc
     5041 ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc agcttgccat
     5101 tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg ctgataatca
     5161 tcaaccggtc gccacgcagc gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt
     5221 cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agctgcatcc cacgccggat
     5281 ccattcctgc cgtggtgttc aacagacgtt gagccgccgg gctacgtccg cttttaatca
     5341 ccaaaatcgg tttattgcgc gaggcactac gggctgccga aacaaagcgt cgcgcgtcgc
     5401 ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgcgcc agatagtcga
     5461 gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag gaaaagccca
     5521 tctcacgctg ttgcgcccag tcgaggatgg tgttggagac agcagccgat tgcgaaataa
     5581 acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga ccttgccagg
     5641 gagccagtaa gcccagactg tttggtccta gcaggcgcat gttatggcgc agggcgcagg
     5701 cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt ttacagcctt
     5761 tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat aaaaccgcaa
     5821 ggtcgggtgt aaagggcaag ttggcaatat ccggccaggc caacacaccc aacaccgctt
     5881 tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc aggttacgca
     5941 tcatcaggta acctgcgcga ttgggtttca tcgatgcgcc aattaccgca atcgattttg
     6001 gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga agtgaccgaa
     6061 tgattttaaa cgctttctaa ctgttctgct gtgatgcttc ccggatgttg cgtttttcct
     6121 gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgtcgc cgccctggta
     6181 tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg gccttcagcc
     6241 tgggcttcac gcaggcgata ggcagaaagc cgagaaagat cgacggaaat gacgggaaga
     6301 ttatccagat acggactttt acgaaacatc ttgcgagctt ctggccaggt accatcgagc
     6361 atgataaata gcggaggctt atcggcaggt ggtgtgaaga tcacttcccg ttgctcatca
     6421 gcatacgaag cgggaaagac caccattggc tgatagtctg ggttttgcac cagatccagc
     6481 aaatcttgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg caaaatatca
     6541 gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat cagcaaacag
     6601 aagtgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt ctctggcaaa
     6661 agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc
     6721 gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg tcataagaga gcgtcgaaaa
     6781 aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa gattaaagag
     6841 attcgttcag ccagctgtcg aacggcgctt tcggtactgc gccattaagc atgtcgacaa
     6901 cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag cgactgctca
     6961 attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta cgctcttgcg
     7021 cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt gcccagaagt
     7081 cgatcaccac aggtagatca tctttcagca atttgtcgag cgtttcaccg gtcgcattaa
     7141 tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc
     7201 gatcgtcggg aatgcgattg atggcctgac aatgggtaca aacggtattc ataactaacc
     7261 tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat atgttacata
     7321 ttatcgggga cattgttcca gacaacaatc tgttttattc aatgggtatg atagacacag
     7381 gctttggaac gaaatcatgg cgaaccgcgc gcacttcggg taatctgcgc gcttcgcgca
     7441 gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg
     7501 atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg taccgggaaa
     7561 gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac
     7621 aaacagagtc agcaccgtga ccgcaagtgg gaagattcgc cgtggcgcac ggtttcccgc
     7681 gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg taaaagtttt
     7741 attgatccgg aagttttgcg tcgtcagcgt gcggaagaaa cccgcgtcta cggcgaaaac
     7801 gcctgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg gtttatccag
     7861 agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg caaagcgtac
     7921 catgtggtgg atgaagcgga actgacaaaa gcgtcaggca cggaacatca cggcggcgtt
     7981 tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag ccaggcaggc
     8041 gcgcaggatt gtgtactggc cctggaaaac gaatctaacc cgcataacct gggtggcatg
     8101 atgcgtagct gtgcgcactt cggcgtgaaa ggtgttgtgg tgcaggatgc agcactgctg
     8161 gagtctgggg cggctatccg taccgcagaa ggcggcgcag agcacgttca gccgattact
     8221 ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg cgggttacac cgtagtgaca
     8281 acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa aatggtactg
     8341 gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa cgatctgcgc
     8401 gtgaagattg atggtactgg caacgttgcc gggctgaata tctctgtcgc aaccggcgtt
     8461 cttcttggcg aatggtggcg tcagaataaa gcctgataaa tcagccgggt ggcaactctg
     8521 ccatccggca ttttcccgca aatttactca ctctctgccg gtaatactgg catccagtca
     8581 atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg gttgcagcca
     8641 aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac atgatggcgt
     8701 tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa aaacaccacg
     8761 ccttcgcgat gctgatttat gaggctgatg actttatcag taaacgtctc ccagccgagg
     8821 ctggcgtggg aatgcgcctg accagcgcga accgtcaaga cggtattgag cagcagaaca
     8881 ccctgacgcg cccagctttc aagataacca tgattagggc gggtgaaacc gggaatagta
     8941 ttttccagct ctttatacat attcagcagt gacggtggtg tcgcaatgcc ggggcgaacg
     9001 gaaaatgcca gaccatgcgc ctgtcctggt ccgtgataag gatcctgacc gagaatcacc
     9061 actttaacgt cacccaactc cgtaaagcgg aacgcgttaa agacatcttt ttgtggtgga
     9121 tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt aagaaaatag
     9181 ggttgctgct tctcttcagc cagcacatca tgccaggtta attcgttagc catctcgctc
     9241 tcctgcgaat ctttaatccg cctagcttaa ctgcttcttt ctacagaaca aaatcatgct
     9301 cattttatag acaaaaagac atgcaaaaaa atgtaaattg gtaaaatttc acatctgcat
     9361 aagttgatgt aaaacaataa aatccatttg tgaccacact tgatatgtgg tttttattga
     9421 tttaaatcaa agaatcaagg gtgtttgagg agtatatata cactcaagca acaatggttt
     9481 taccaattgg ccgcgacagg ctgaacaaat caaataattt tgccggggag gcatcacatg
     9541 attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc tttctggttg
     9601 ctggacagcg aaaaaggcga agcgcgttgc atcgttgcga aagcaggttt tgcagaagac
     9661 gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc agtagaagtg
     9721 aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct gcgtcgtgaa
     9781 actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat ccgtgtatcc
     9841 ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt tatcgctcgt
     9901 acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag agggcgacct
     9961 cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc aacagggcaa
    10021 gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga tgccccgcca
    10081 gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg acgccaacta
    10141 cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta acaccgtaca
    10201 aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg attggttttg
    10261 cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc cagacaatat
    10321 atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact gccagtgaaa
    10381 atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc cctgccagca
    10441 cacgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga atattgttcg
    10501 gtcctggcgt catggcggta atcagggtgt aagtccaaaa agcacttaaa agggtcggtg
    10561 tcacttgtta tctcctctgt tttggtagac agtacccgac acggaacatt taaaaaaatg
    10621 aatgttttta cattctaaat tcgatatttt cgataggttt ggggttatgg atctgcgccg
    10681 ttttattacg cttaaaaccg tggtggaaga gggttccttt ttgcgagctt cgcaaaaatt
    10741 gtgctgtaca caatcgacgg tgacttttca tattcagcag cttgagcagg agttctcggt
    10801 acaattgttt gagaaaattg gtcgacgaat gtgccttacc cgcgaaggaa aaaagctgct
    10861 gccgcacatc tatgaactta cccgagtgat ggatacgctt cgtgaggctg ctaaaaaaga
    10921 gtcggatccg gacggggaac tacgcgttat ttcaggcgaa acgttgctct cttatcgaat
    10981 gccgcaggtg ttgcagcgtt ttcggcaacg cgctccaaaa gtgcgtttat cgttgcagtc
    11041 gctgaactgt tacgtgatcc gtgatgcgct actgaatgat gaggccgatg tcggggtttt
    11101 ttatcgcgta gggaatgatg atgctctgaa ccgacgagag ttgggtgaac aaccgctggt
    11161 gctggtggct tcaccgcaaa ttgcagatgt cgattttact gagccgggaa gacataacgc
    11221 ctgtagcttt attatcaacg aaccacaatg tgtcttccgg cagatatttg agagcacgct
    11281 gcgccagcgg cggatcacgg tggaaaacac cattgagctt ataagtatcg aaagcatcaa
    11341 gcgttgtgtc gcggcaaata tcggagtcag ttacctgccg cgttttgcgg tagtaaaaga
    11401 gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa cagtcgcaga ccattacggc
    11461 aatgtgcgct caccatgccg gaaaagcggt tagcccggcg atgcacactt ttattcagtg
    11521 tgttgaagag tgttttttgc cgggataaaa acaatgccgg gcttaaaccc ggcattgaga
    11581 ttactcttct gtcgtttgcg gtggcacgcc cgttccgctt ggtttacggc gcttaccaat
    11641 atttttagtg tcgcgatggc gttttttcac ccgcggcttc tctttctctt tagctttttt
    11701 cttctcggca cgtttagcca acactttctt cgatggcttg ccggtctgct tctcgcttgg
    11761 cgcacgcgtt ttcggacgta actcatcaat tacgcgagct ttaattggct cttcaatata
    11821 acggccaact ttgcccagca gcagatggtc atgggcttcc accagcgaaa tcgcggtgcc
    11881 tttacgaccg gcgcgcgcgg tacgtccgat acggtgcaaa taagtatcgc cactgcgcgg
    11941 catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg ataccgcgcg cggcaacatc
    12001 ggtagcaacc agtacgttta cgcggccttc ggtcaagcgt ttaatcgctt cgttacgctt
    12061 gccttgtacc atctcacctt cgagatagca gttgttgatg cctgcttcgc gcagccagtt
    12121 tgccagctca tgcacacgct cacgcttacg cacaaacaca attgagcggg tcgcttccgg
    12181 ctgttttaac agatgcacca gcaacgcggt tttatgctca agatcatcgg cgcggtaata
    12241 ccactgatga atttttttgc gctcacgggt ggacggattg gcagaaactt ccaccggatc
    12301 ttccaacaga cgctcggcaa agtcctgaat cgcatcgcct tccagcgttg ccgaaaagag
    12361 cagggtctgt ttacgccagc gcgtttcgcc agcaatatgt tcgatatcct gggcgaagcc
    12421 catatccagc atacggtctg cttcgtcgag gatcagcgtt tcaaccgcgc ggcaatcgaa
    12481 gttctcttct tttatgtatt gcagcagacg tccggtcgtg gcgaccacaa tgtcctggtt
    12541 ttcgctgaac acttctgcgt ggttcatata ggccacgccg ccggtgatgg tggcaatatc
    12601 cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc acctgcatcg ccagctcgcg
    12661 agttggggtg aggatcaaaa tacgcggcgg accggatttc ttacgtggga aatcgagcag
    12721 gtgctgcaac gctggcagca gatacgccgc cgttttaccg gtgcctgtcg gcgcagaacc
    12781 gagtacatca cggccatcga gcgcaggcgg aatggcggca gcctgaatgg cggtcgggcg
    12841 agtgaaacct ttatcctgga gggcttccag caggctttcg tcgagttcaa gttcggaaaa
    12901 agtcgttaca gtcatgttct acctctgtgt ggggcgctga ttatagacgt tacggctgca
    12961 atcttcatct gtttgtatgg atatcgcttc tcgggtattg ttctcacccg gtgctatcgc
    13021 cgtttttcct gcaaggtttt attttcatgt cacagtctac atccgtgttt cgtcgtaatg
    13081 gatttacttt taaacagttt tttgttgctc acgatcgctg tgcgatgaaa gtgggaacgg
    13141 atggcatttt gctgggcgca tgggcaccgg tagctggggt aaaacgttgc cttgatatcg
    13201 gcgcgggtag cgggttgctg gcattaatgc tggcgcagcg aacagatgac agcgtgatga
    13261 ttgatgcagt tgaactggaa agtgaagctg ccgcgcaagc gcaggaaaat atcaaccagt
    13321 ccccgtgggc agagcggatt aatgtccata cggcggatat tctgcagtgg atcacacagc
    13381 agacagtacg cttcgattta atcatcagca acccacctta ctatcagcag ggagtagagt
    13441 gcgcgacacc tcaacgggaa caggctcgct ataccactac gcttgatcac ccatcgttgc
    13501 tgacttgtgc ggcggagtgc attaccgaag agggattttt ctgcgtggtg ctgccagagc
    13561 agattggtaa tggttttact gaactggcat taagtatggg ctggcattta cgtttgcgga
    13621 cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt gctgctggca ttctccccgc
    13681 aggcgggaga gtgctttagc gatcgcttag tcattcgtgg gccagaccag aactattccg
    13741 aagcgtatac ggcgctgacc caggcttttt atctgtttat gtaatgattg ccggcagaaa
    13801 ggatcgatgg accggaatgc gtgagcagtt ccggataatc cagcgtgaaa tgcaggccac
    13861 ggctctcctt gcgcatcatc gcacagcgaa caatcaactc ggcaacctgt accagattac
    13921 gcagctccag caaattattt gagacgcgga aatgggcgta atattcgtct atttcttgtt
    13981 ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt cgttgtgcga acaatgccaa
    14041 cgtaatccca cataaacaga cgtagctcgt gccagttatg ctgaattact acccgttcgt
    14101 cagggttctc aacgcggctt tcatcccacg gcggtaacgt actgacgccg tgggcataag
    14161 gtatacgtct gctgatatct tctgccgccg accagccata gaccagacac tccagcaatg
    14221 aattcgaggc catgcggtta gcgccgtgta agccggtata actcacttcg ccaatggcat
    14281 acaaaccctc gacgtccgta cgcccatgat catcaaccat tacaccaccg caggtataat
    14341 gtgcagcagg cacaatcggc acgggttctt tcgtgagatc tatccccagc ccgagcaatt
    14401 tttcataaat catcgggaaa tgctggcgaa taaaatcggc gggcttatgg ctgatgtcga
    14461 ggaacataca atctgcgccg aggcgtttca tttcatggtc aatggcgcgg gcgacaatat
    14521 cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat aaaacgcgtg ccatccgggc
    14581 gcttgagata agcgccttcg ccgcgcagtg cttccgttaa caggaaattg cgtgcctgtg
    14641 ggtgatacag cgcggtaggg tggaactgat taaattcgag attggcaacc cggcagcctg
    14701 cgcgccacgc catagcaatg ccatcgccag aagaaatatc cggattggtg gtgtactgat
    14761 aaacttttga agcaccgccg gttgccagta ccactgcttt tgcgtggcag gtttcaaccg
    14821 tttctttatt tcggttccat acccacgcgc caacaacccg tcgcgtgccc ggcaggccaa
    14881 ttttgtcaga aacaatcaga tcaaccgcgt tgctgcgctc cagcacgcga atattcggat
    14941 ggttctgcgc cttgcccacc agcgtggttt ctacttctct accggtggcg tcggcggcat
    15001 gaagaatacg acggtgacta tgtccacctt cacgagtcag atggtagctt tcttcgccat
    15061 tcggctgaat gtgggtatca aacaacaccc cctggtcgat tagccattgc acacaggatc
    15121 gtgcattact ggcgacaaat tcaactgcat ggcgatcgca aataccagcc ccggcaatca
    15181 atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc aaacacggcg gcaataccgc
    15241 cctgggcata aaatgttgaa ccttccgtta ccgggccttt acttagaacg atgacctgat
    15301 gctggtcagc caggcgtagc gccagtgaaa gtccagctgc gccgctaccg ataatcaaca
    15361 cgtcacatga atgttcaggg agagtattca ttttctttgt ttaatttact aaacactgtt
    15421 tggtcagcat aacatcatgt tgtgcaaata aacacctgct attttaatat ttgttacagt
    15481 gactaaacac gctggctcag ggcggcgaga aaaagagaag ttactggctg gtggaggatt
    15541 aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc agttaaatgg gcatttctac
    15601 acagataatg cgatgttcag attctgtaga cttataatga tagataatga tccgtctaca
    15661 gcatgacaaa caaaaacaga tgcgttacgg aactttacaa aaacgagaca ctctaaccct
    15721 ttgcttgctc aaattgcagc taatggagtg gcgtttcgat agcgcgtgga aatttggttt
    15781 ggggagactt tacctcggat gagcgagcag ttaacggacc aggtcctggt tgaacgggtc
    15841 cagaagggag atcagaaagc ctttaactta ctggtagtgc gctatcagca taaagtggcg
    15901 agtctggttt cccgctatgt gccgtcgggt gatgttcccg atgtggtaca agaagctttt
    15961 attaaagcct atcgtgcgct ggattcgttc cggggagata gcgcttttta tacatggctg
    16021 tatcggattg ctgtaaatac agcgaaaaat tacctggttg ctcaggggcg tcgtccacct
    16081 tccagtgatg tggatgccat tgaagctgaa aacttcgaaa gtggcggcgc gttgaaagaa
    16141 atttcgaacc ctgagaactt aatgttgtca gaagaactga gacagatagt tttccgaact
    16201 attgagtccc tcccggaaga tttacgcatg gcaataacct tgcgggagct ggatggcctg
    16261 agctatgaag agatagccgc tatcatggat tgtccggtgg gtacggtgcg ttcacgtatc
    16321 ttccgagcga gggaagctat tgataacaaa gttcaaccgc ttatcaggcg ttgacgatag
    16381 cgggatactg gataagggta ttaggcatgc agaaagaaca actttccgct ttaatggatg
    16441 gcgaaacgct ggatagtgag ctgcttaacg aactggctca taacccagaa atgcagaaaa
    16501 cctgggaaag ctatcactta atccgtgact caatgcgggg tgatactccc gaggtgctcc
    16561 atttcgatat ctcttcacgc gtgatggccg ccattgaaga agagccagta cgtcaaccgg
    16621 cgacattgat cccggaagcc cagcctgcgc cgcatcaatg gcagaaaatg ccattctggc
    16681 agaaagtacg tccgtgggcg gcacagctta cccaaatggg cgtagcagca tgcgtatcgc
    16741 ttgcagttat cgttggcgtc cagcactata atgggcaatc tgaaacgtcc cagcagcccg
    16801 aaacgccagt atttaataca ctgccgatga tgggtaaagc cagcccggtt agtctgggag
    16861 taccttctga agcgaccgca aataatggtc aacagcagca ggtacaggag cagcgtcgtc
    16921 gcattaatgc aatgttgcag gattacgaac tgcaacgccg actccactct gaacagcttc
    16981 agtttgagca ggcacaaact cagcaagccg ctgtacaggt gccaggaatt caaactttag
    17041 gaacgcaatc gcagtaatga agcaactttg gtttgccatg tcattagtga caggtagcct
    17101 gttattctct gctaacgcct tggccactcc cgcgtccggg gcgttattac agcagatgaa
    17161 cctggccagt cagtcactga attacgagct gtcatttatc agcatcaata aacagggtgt
    17221 tgagtctctg cgttatcgac atgcacgcct cgataaccgt cctcttgcac aattgttgca
    17281 aatggatggc ccgcgccggg aagtggtaca gcgcggcaat gaaatcagct attttgaacc
    17341 gggacttgaa ccgttcacgc ttaatggcga ttacattgtt gattctctgc catcgctcat
    17401 ctataccgat ttcaaacgcc tttctcctta ctacgacttt atctctgtcg ggcgcacgcg
    17461 tattgctgat cgtctttgcg aagtcattcg tgtggttgcc cgagatggta cacgctacag
    17521 ctacatcgtg tggatggaca ccgaatcgaa attaccgatg cgggttgatc tccttgatcg
    17581 cgatggtgaa acgctggaac aatttcgcgt gattgctttt aacgtcaatc aggatatcgg
    17641 cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg ttgctttctg ttcctgttgg
    17701 tgaaaaagct aaattcagct ggacgccaac ctggctgcca cagggtttta gcgaagtttc
    17761 cagtagtcga cgtccgctac cgacgatgga caacatgcct atcgaatcac gtctctattc
    17821 cgacggatta ttcagcttct cggtaaacgt taaccgcgct acgccatcga gcaccgatca
    17881 gatgttgcgc accggacgca gaaccgtcag tacaagcgta cgtgataacg ccgaaatcac
    17941 cattgttggt gaactgccgc cgcaaacggc gaaacgcatt gccgagaata ttaagttcgg
    18001 gacagcgcaa tgatcaaaga gtgggctacc gtcgtctcct gggaaaacgg gcaggcgctg
    18061 gtcagttgtg atgttaaagc ctcatgcagc agctgcgctt cacgcgccgg ttgcggtagc
    18121 cgtgtgttga ataaacttgg cccgcaaacc acgcatacca ttgtcgtacc ctgtgatgag
    18181 ccgctagtgc cggggcaaaa agtggaattg gggatcgccg aaggcagcct tcttagctcc
    18241 gcattactgg tttatatgtc gccgctggtg ggattattcc tcatcgcttc gctatttcag
    18301 ctactctttg cttctgacgt cgcagcatta tgtggtgcga tcctcggcgg tattggtggg
    18361 ttcctgattg cgcgcggcta ttcgcgtaag tttgctgccc gggcagaatg gcaaccgatc
    18421 atcttaagcg tggcattgcc gcctggcctt gtgcgatttg aaacatcttc ggaagacgcg
    18481 agccagtgat ttcactggct ttgttgcatt ttgcataaca aatgcgagga cgtttccata
    18541 taccacaatc catacggtat gaacatttcc tcgcctcaat gttgtagtgt agaatgcggc
    18601 gtttctatta atacagacgt taagctcaga acagcgactt ctaaaagcct ggttaaccgg
    18661 gcattaaggc acaataatca tactttatga agaatatacg taacttttcg atcatagctc
    18721 acattgacca cggtaaatcg acgctgtctg accgtattat ccagatctgc ggtggcctgt
    18781 ctgaccgtga aatggaggcg caggttctcg attccatgga tcttgagcgt gagcgtggca
    18841 ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc gtctgacggc gaaacctatc
    18901 agcttaactt tatcgacacc ccgggccacg tagacttctc ctatgaagtt tctcgttcgc
    18961 tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg gcagggcgta gaagcgcaaa
    19021 ccctggcaaa ctgctacacc gccatggaaa tggatctcga agtggtgccg gtactgaaca
    19081 agattgacct gccggcagcc gatcctgaac gcgtggcgga agaaattgaa gatatcgtcg
    19141 gcatcgacgc caccgacgcg gtgcgctgtt cagcgaaaac cggcgttggc gtgcaagacg
    19201 ttctcgaacg cttggtgcgc gacattccgc cgccggaagg cgatccggaa ggcccattgc
    19261 aggcgctgat tatcgactcc tggttcgaca actacctggg cgttgtttca cttatccgta
    19321 ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt catgagcacc gggcagacct
    19381 ataacgccga tcgtctgggc atcttcacgc cgaagcagat tgaccgcact gaactgaaat
    19441 gtggcgaagt aggctggctg gtatgtgcga ttaaagatat ccacggcgct ccagtaggcg
    19501 ataccttaac actggcgcgt aatccggcag aaaaggcgct gcctggcttt aagaaagtca
    19561 aacctcaggt atatgctggt ctgttcccgg taagttccga cgactatgaa gccttccgtg
    19621 acgcgctggg taaactcagc ctgaacgatg cctcactgtt ctatgagccg gaaagctcca
    19681 gcgcgttggg ctttggtttc cgctgcggct tccttggcct gctgcacatg gagatcatcc
    19741 aggaacgtct ggaacgtgaa tacgatctgg atctgatcac cactgcgccg actgtagtgt
    19801 atgaagttga aaccacgtca agagaagtta tctacgtcga cagcccatcc aagctgccag
    19861 cggtaaataa catttacgaa ctgcgcgagc cgattgcaga gtgtcacatg ctgctgccgc
    19921 aggcatatct cggcaacgtt attacgctgt gcgtagaaaa acgcggcgtg cagaccaata
    19981 tggtttacca cggtaatcag gtggcgctga cgtacgagat cccgatggcg gaagtggtgc
    20041 tcgacttctt cgatcgcctg aaatctacct cgcgtggtta tgcgtctctg gattacaact
    20101 tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt attaatcaac ggtgaacgtg
    20161 ttgatgcatt ggcgctgatc acccaccgtg gtaattcgca aaaccgcggt cgcgagctgg
    20221 tggagaagat gaaagatctg atcccacgcc agcagtttga tatcgccatt caggcagcga
    20281 ttggtacgca catcattgcg cgctccaccg tgaaacagct gcgtaaaaac gtactggcta
    20341 aatgttatgg cggcgatatc agccgtaaga aaaagctgct gcagaagcag aaagaaggta
    20401 agaaacgcat gaagcagatc ggtaacgtcg agctgccgca ggaagcgttc ctcgccattc
    20461 tgcacgtcgg caaagacaac aaataaccct taggagttgg catggcgaat atgtttgccc
    20521 tgattctggt gattgccaca ctggtgacgg gcattttatg gtgcgtggat aaattctttt
    20581 tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc ggctgccggt gactcactgg
    20641 ataaagcaac gctgaaaaag gttgcgccga agcctggctg gctggaaacc ggtgcttctg
    20701 tttttccggt actggctatc gtattgattg tgcgttcgtt tatttatgaa ccgttccaga
    20761 tcccgtcagg ttcgatgatg ccgactctgt taatcggtga ttttattctg gtagagaagt
    20821 ttgcttatgg cattaaagat cctatctacc agaaaacgct gatcgaaacc ggtcatccga
    20881 aacgcggcga tatcgtggtc tttaaatacc cggaagatcc aaagcttgat tacatcaagc
    20941 gcgcggtggg tttaccgggc gataaagtca cttatgatcc ggtctcaaaa gagctgacga
    21001 ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc gctaccggtc acttactcca
    21061 acgtggaacc gagcgatttc gttcagacct tctcacgccg taatggtggg gaagcgacca
    21121 gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa tggaattcgt ctttccgagc
    21181 gtaaagagac actgggtgat gtgacgcacc gtattctgac agtgccgatt gcgcaggacc
    21241 aggtggggat gtattaccag cagccagggc aacaactggc aacctggatt gttccgccgg
    21301 gacaatactt catgatgggc gacaaccgcg acaacagcgc ggacagtcgt tactggggct
    21361 ttgtgcctga agcgaatctg gtcggtcggg caacggctat ctggatgagc ttcgataagc
    21421 aagaaggcga atggccgaca ggtgtgcgct taagtcgcat tggcggtatc cattaatagc
    21481 catcttcgtt cacgttgtcg ccgttatggc gacaacgtga attatttatg agataaatct
    21541 cccgtggcta acgacatccc ccgtcgttgt gtatagaata ttcccccgaa gtttaaggtt
    21601 ggcacctcca ggttgccacg gcacacgaaa cagcgttggt ccccatatac cggtaaactg
    21661 aaactgcagt gaagcagtta gccgagccat gtatatcagg tctgtttcgt gtgctgaatt
    21721 gttgacgcat ttatttattg gtatcgcatg aaccccatcg taattaatcg gcttcaacgg
    21781 aagctgggct acacttttaa tcatcaggaa ctgttgcagc aggcattaac tcatcgtagt
    21841 gccagcagta aacataacga gcgtctggaa tttttaggtg actctattct gagctacgtt
    21901 atcgccaatg cgctttatca ccgtttccct cgtgtggatg aaggcgatat gagccggatg
    21961 cgcgccacgc tggtccgtgg taatacgctg gctgaactgg cgcgcgaatt tgagttaggt
    22021 gagtgcttac gtttagggcc aggtgaactt aaaagcggtg gatttcgtcg tgagtcaatt
    22081 ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc tcgacagtga tattcaaacc
    22141 gtcgagaaat taatcctcaa ctggtatcaa actcgtctgg acgaaattag cccaggcgat
    22201 aaacaaaaag atccgaaaac gcgtctgcag gaatatttgc agggtcgcca tctgccgctg
    22261 ccaacttatc tggtagtcca ggtacgtggc gaagcgcacg atcaggaatt tactatccac
    22321 tgccaggtca gcggcctgag tgaaccggtg gttggcacag gttcaagccg tcgtaaggct
    22381 gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc tggaatgagc atcgataaaa
    22441 gttactgcgg atttattgct atcgtcggac gtccgaacgt tggcaaatcc acattgttga
    22501 acaaactgct ggggcagaag atctccatta cctcccgcaa ggcgcaaaca actcgtcacc
    22561 gcattgtggg gatccatact gaaggcgcgt atcaggcgat ctacgttgat accccaggcc
    22621 tgcatatgga agaaaaacgc gccattaacc gtctgatgaa caaagcggcg agcagctcca
    22681 ttggcgatgt tgagctggtg atttttgtcg tagaaggcac tcgctggacg ccggacgatg
    22741 aaatggtgct caacaaactg cgcgatggca aagcgccggt aatcctcgcg gtgaacaaag
    22801 tggataatgt gcaggaaaaa gccgatctgc tgccacactt acagttcctg gcaagccaga
    22861 tgaacttcct cgatatcgtg ccgatctctg ccgaaaccgg gctgaatgtc gacactattg
    22921 cagcaatcgt gcgtaagcat ctgcctgaag cgacccatca cttcccggaa gattacatca
    22981 ccgatcgctc acagcgtttt atggcgtctg aaattatccg cgaaaaactg atgcgttttc
    23041 tcggcgctga actgccgtac tccgtgaccg tggagatcga acgtttcgtc tctaacgaac
    23101 gcggcggtta tgacatcaac ggtttgattc tcgttgagcg tgaagggcag aagaagatgg
    23161 tcattggcaa caaaggggcc aagatcaaaa ccatcgggat tgaagcgcgt aaagacatgc
    23221 aggaaatgtt cgaagcgcct gttcaccttg agctgtgggt aaaagtgaaa tccggttggg
    23281 ccgacgacga acgcgcactg cgcagtctcg gttatgttga cgatctttaa gagtaactcc
    23341 gatggaaggc tggcagcgcg catttgtcct gcatagtcgc ccgtggagcg aaaccagcct
    23401 gatgctggac gtcttcacgg aggaatcggg tcgcgtgcgt ctggttgcca aaggcgcacg
    23461 ctctaaacgc tctactctga aaggtgcatt acagcctttc acccctctct tgctacgttt
    23521 tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa gctgtctcgc tggcgctgcc
    23581 attaagcggt atcacgcttt acagcggtct gtacatcaac gaacttctct cccgcgtact
    23641 ggaatacgag acgcgcttct ctgaactctt tttcgattac ttgcactgca ttcagtctct
    23701 tgccggggtc actggtacgc cagaacccgc gttgcgccgc tttgaactgg cactgctcgg
    23761 gcatctgggc tatggcgtca attttaccca ttgtgcgggt agcggcgagc cggtagatga
    23821 caccatgacg tatcgttatc gcgaagaaaa agggtttatc gcaagcgtcg ttatcgacaa
    23881 taaaacgttc accggaaggc agttaaaagc gttaaacgcg cgggaatttc ctgacgcaga
    23941 cacactgcgc gccgcgaaac gctttacccg catggcgctt aagccgtatc ttggcggtaa
    24001 accattaaag agcagggaac tgttccggca gtttatgcct aagcgaacgg tgaaaacaca
    24061 ttatgaatga tgaggattgt catggctgaa ttactgttag gcgtcaacat tgaccatatt
    24121 gcaacactgc gtaacgcgcg cggtactgct tacccggatc cggtgcaggc ggcgtttatt
    24181 gctgagcagg cgggagcgga cggcattacc gtgcatttac gtgaagaccg ccgccacatt
    24241 accgaccgcg atgtgtgcat cctgcgccag acgctggata cccgcatgaa tctggagatg
    24301 gcggtgaccg aagagatgct ggcgatcgcc gttgagacga agccacattt ttgctgtctg
    24361 gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc tggacgtcgc agggcagcgt
    24421 gaaaaaatgc gcgatgcctg caaacgtctg gcagatgccg ggatccaggt ttctctgttt
    24481 atcgacgccg atgaagagca gatcaaagct gcggcagagg ttggcgcacc gtttatcgag
    24541 atccacaccg gttgctatgc tgatgccaaa actgacgccg aacaggcgca agagttggcg
    24601 cgtatcgcca aagccgcgac ctttgccact agtctcggtc tgaaagttaa cgccggacac
    24661 ggtctgacct atcacaacgt gaaagccatt gccgccatcc ctgagatgca tgaactgaat
    24721 atcggtcatg ccattattgg tcgtgcagtg atgaccggac tgaaagatgc ggtggcagaa
    24781 atgaagcgtc tgatgctgga agcgcgtggc taatggcaat attaggttta ggtacggata
    24841 tcgtagagat cgctcgcata gaagcggtga tcgcccgatc cggtgagcgc ctggcacgcc
    24901 gcgtattaag cgataacgaa tgggaaatct ggaaaacgca ccaccagcca gtgcgttttc
    24961 tggcgaagcg ttttgctgtg aaagaagcgg cggcaaaagc atttggcaca gggatccgca
    25021 atggtctggc gtttaatcag tttgaagtat tcaatgatga gctcggcaaa ccacggctac
    25081 ggctatgggg tgaggcatta aaactggcgg aaaagctcgg cgttgtaaat atgcatgtaa
    25141 cgctt
//
LOCUS       DAJOTB010000062        25107 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJOTB010000062 DAJOTB010000000
VERSION     DAJOTB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.062"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1157
                     /locus_tag="OHN28_002464"
     CDS             <1..1157
                     /locus_tag="OHN28_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297852.1"
                     /translation="YAENAEKMAEQHKDKKYYDAVKSLYMTSAVIYEKNGEYERAKRC
                     KLASANITIKVATDRPDNMGKISWLRTAISELRQYGGDRQQIEDLKEQLANLREEVRS
                     EYVTLSHSIDISDLVAESEKKLTGRSLPDIFKVLMRETPIENIESMREQVLRLSEKSL
                     FSSFVGTEINDESGRKVYTIPPLDNKKDMSDDVVIDQYLRNFEITHKIFVNGVFEPAR
                     YVLSQEHGLTLSTFVKLVTTSPIVKPEFREIFNLGFYKLWQGDYISAAYLLIPQMEGM
                     VRYYYELSGKDATRYLDKGLEESTSISQLLDKCRDDLESIFSKNLVLTIDVLFNRKSG
                     ATLRHKLAHGNLYTNACYDETTTYACILIFFLCAYPLLPYFDTVFEQGSV"
     gene            complement(1497..2531)
                     /locus_tag="OHN28_002465"
     CDS             complement(1497..2531)
                     /locus_tag="OHN28_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2297853.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(2531..4303)
                     /locus_tag="OHN28_002466"
     CDS             complement(2531..4303)
                     /locus_tag="OHN28_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCQ2297854.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            4477..5331
                     /locus_tag="OHN28_002467"
     CDS             4477..5331
                     /locus_tag="OHN28_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCQ2297855.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            5390..6463
                     /locus_tag="OHN28_002468"
     CDS             5390..6463
                     /locus_tag="OHN28_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCQ2297856.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTSDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            6467..7210
                     /locus_tag="OHN28_002469"
     CDS             6467..7210
                     /locus_tag="OHN28_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCQ2297857.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     SQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            7310..7819
                     /locus_tag="OHN28_002470"
     CDS             7310..7819
                     /locus_tag="OHN28_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCQ2297858.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            7819..8022
                     /locus_tag="OHN28_002471"
     CDS             7819..8022
                     /locus_tag="OHN28_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCQ2297859.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            8026..8307
                     /locus_tag="OHN28_002472"
     CDS             8026..8307
                     /locus_tag="OHN28_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCQ2297860.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            8307..8804
                     /locus_tag="OHN28_002473"
     CDS             8307..8804
                     /locus_tag="OHN28_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HCQ2297861.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            8819..9244
                     /locus_tag="OHN28_002474"
     CDS             8819..9244
                     /locus_tag="OHN28_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HCQ2297862.1"
                     /translation="MKKLSLSLMRNVSLALMLALSLIYPQSVAVNFVAAWAILATVIC
                     VVAGGFGVYATEYVLERYGRELPPESLAVKIVASLFLQPVPWRARAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            9232..9657
                     /locus_tag="OHN28_002475"
     CDS             9232..9657
                     /locus_tag="OHN28_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCQ2297863.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANSVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQALENAAKREAQREKTITRLLNENEDFRRW
                     YGAGLPDAVRRLHQRPACTDASDCPQRLPESEPLPDAGQ"
     gene            9644..9802
                     /locus_tag="OHN28_002476"
     CDS             9644..9802
                     /locus_tag="OHN28_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HCQ2297864.1"
                     /translation="MPGSDPETNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            9765..10232
                     /locus_tag="OHN28_002477"
     CDS             9765..10232
                     /locus_tag="OHN28_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297865.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            10225..10677
                     /locus_tag="OHN28_002478"
     CDS             10225..10677
                     /locus_tag="OHN28_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCQ2297866.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            10744..11379
                     /locus_tag="OHN28_002479"
     CDS             10744..11379
                     /locus_tag="OHN28_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCQ2297867.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSEGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            11376..11723
                     /locus_tag="OHN28_002480"
     CDS             11376..11723
                     /locus_tag="OHN28_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCQ2297868.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            11728..12636
                     /locus_tag="OHN28_002481"
     CDS             11728..12636
                     /locus_tag="OHN28_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCQ2297869.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            12629..13159
                     /locus_tag="OHN28_002482"
     CDS             12629..13159
                     /locus_tag="OHN28_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCQ2297870.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVKE
                     WWETNDPPGTFRLDIGVLESGITEEMYLEMERLIADAKPASRHLIGLNIIQDIPGYLY
                     TGGVVCDGDVITVYPG"
     gene            13170..15788
                     /locus_tag="OHN28_002483"
     CDS             13170..15788
                     /locus_tag="OHN28_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297871.1"
                     /translation="MSTKFKTVITTAGAAKLAAATVPGGKKVNLSAMAVGDGNGQLPV
                     PDAGQTKLVHEVWRHALNKVSVDNKNKNYIVAELVVPPDVGGFWMRELGLYDDAGTLI
                     AVSNMAESYKPELAEGSGRAQTCRMVIILSNVASVELSIDASTVMATQDYVDDKIAEH
                     EQSRRHPDATLTEKGFTQLSSATNSTSESLAATPKAVKAANDNANSRLAKNQNGADIQ
                     DKSAFLDNVGVTSLTFMKNNGEMPVDADLNTFGPVKAYLGIWSKATSTNATLEKNFPE
                     DNAVGVLEVFAAGNFAGMQRFITRDGNVYMRKLANKWNGTDGPWGVWRHTQSATRPLS
                     TTIDLNTLGAAEHLGLWRNSSSAIASYERNYPEEGGFAQGTLEILEGGNYGRTQRYTT
                     RRGNMYVRCLAASWDASNPQWEPWLRVGHQSESRYYDGDLNDVTSPGIYSVTGKATNG
                     PILDGNGVTVLGILEVLRRFDGVNVWQRYTTAGTGATLKGRTFERVYTGSSWSEWREV
                     YTSYSLPLNLGIGGAVAKLTSLDWQTHDFVPGSLTTVRLDNMTNIPDGMDWGVIDGNL
                     INIAVGPSDDSGTGRSMHVWRSTVSKANYRFFMVRISGNPGSRTITTRRVPIIDEAQT
                     WAAKQTFSAGISGELSGNAATATKLKTPRTINGVKFDGSANIEAFPPGVPLPWPSDTP
                     PAGYAIMQGQTFDKAAYPKLAIAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSH
                     THSASASNTDLGTKTTSSFDYGTKTVSTFNHGTKSTNTSGNHTHTVGFAVSVQSGGAD
                     YGVPRSDKGATTTSSSGNHAHTVDIGAHNHTVGIGAHSHTVAIGSHGHSITVNATGNT
                     ENTVKNIAFNYIVRLA"
     gene            15788..16366
                     /locus_tag="OHN28_002484"
     CDS             15788..16366
                     /locus_tag="OHN28_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2297872.1"
                     /translation="MAFRMSAEAQTIRVFNLLDGTNEFIGESDAYIPPHTGLPANSTD
                     IAPPDIPAGYAAVFNADEMKWELMEDHRGKTVYETKTGVAIYISELGVLPPDVTAISP
                     DGDYQKWNGNAWVNDENAERDALVRAADSQKKELIAYAGEIIATLQDAVDLDMATEEE
                     KLSLTHWKKYRVLLNRVQPEDAPDIEWPEMPQ"
     gene            17061..17771
                     /locus_tag="OHN28_002485"
     CDS             17061..17771
                     /locus_tag="OHN28_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCQ2297873.1"
                     /translation="MTKWTFEKNIVAQGIAAKNKSGRIPAIDIQPGSGLLKRHQMALY
                     ESAINFRAPIEENKCGRYNPPGGCVAVWYASDSALTAAAEAYGRLLHLEGTISYPESV
                     LSQHYMCSVDVMRPVKVIDVVALCELLHIPLDSLENEDYTFTQWLMEHLYTHFGRDYD
                     GIAYTSRHKRYKRCYAFWERPDSTPAFTDTPQGMVPYASYIENDEGIFPKGWHKGTMS
                     GEEMFEELLGFEITPEPM"
     gene            complement(17810..18454)
                     /locus_tag="OHN28_002486"
     CDS             complement(17810..18454)
                     /locus_tag="OHN28_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2297874.1"
                     /translation="MIKHTAAERETNTSAKLNEYQLNLLKRINNLAISLMPEFEDKNQ
                     AINAITLDDSSLMQLLYTVQLEQKTRVNDYELRKVRRRRENLERFYRGLQELGGTLKV
                     NDVVDILGITRQAINIRVKKNKLLAFKQNGDFIFPKFQFTDNGLIPGFEDVMAAFDEN
                     THPMLRLGVLKSPIEVNSEGLKKTPIQIMQDGAKNSELSLAIRAAKQFGNHVSS"
     gene            18822..20012
                     /locus_tag="OHN28_002487"
     CDS             18822..20012
                     /locus_tag="OHN28_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCQ2297875.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKSGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMVEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            20025..20543
                     /locus_tag="OHN28_002488"
     CDS             20025..20543
                     /locus_tag="OHN28_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCQ2297876.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDSVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            20600..20875
                     /locus_tag="OHN28_002489"
     CDS             20600..20875
                     /locus_tag="OHN28_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCQ2297877.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            20908..21027
                     /locus_tag="OHN28_002490"
     CDS             20908..21027
                     /locus_tag="OHN28_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCQ2297878.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            21020..23467
                     /locus_tag="OHN28_002491"
     CDS             21020..23467
                     /locus_tag="OHN28_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2297879.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAATVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAVFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKVAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNSCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGSG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            23482..23961
                     /locus_tag="OHN28_002492"
     CDS             23482..23961
                     /locus_tag="OHN28_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2297880.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            23961..>25107
                     /locus_tag="OHN28_002493"
     CDS             23961..>25107
                     /locus_tag="OHN28_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCQ2297881.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYE"
BASE COUNT         6079 a         6360 c         7064 g         5604 t
ORIGIN      
        1 agtatgcgga aaatgctgag aagatggctg agcagcataa agataaaaaa tattatgatg
       61 ctgtaaaatc actttatatg acaagtgcag ttatttatga aaaaaatggt gagtatgaac
      121 gtgccaagag atgtaaactg gcatctgcaa atattactat taaagttgca acagatcgac
      181 ctgataatat gggtaagatc tcttggttaa gaacagctat ctctgaatta agacagtacg
      241 gtggtgatag acaacagata gaagatttaa aggaacaact tgctaatttg agagaagaag
      301 taaggagtga atatgtcact ttaagccatt ctattgatat tagtgacttg gttgctgaaa
      361 gtgaaaaaaa actgacgggg cgttctttgc ccgatatatt caaagtttta atgcgtgaaa
      421 caccgattga aaatattgaa agcatgaggg agcaagtttt aagattatca gaaaaaagtt
      481 tattctctag ttttgttgga acagaaatta atgatgagag tgggcgtaag gtatatacaa
      541 tcccgccgtt ggataataaa aaagatatga gtgatgatgt tgttattgat caatatctaa
      601 gaaatttcga gattactcat aagatttttg taaacggtgt ctttgaacct gctcgctatg
      661 tactttctca agaacatggc ttgacgttgt caacattcgt taaattagtt acaactagtc
      721 caattgtcaa gccggagttt agggagattt ttaatttggg cttttacaaa ttatggcagg
      781 gagattacat cagtgcagct tatcttttaa taccacaaat ggaaggtatg gttcgttatt
      841 attatgagct tagtggaaag gatgctacta gatatttgga taagggatta gaggagtcaa
      901 caagtatttc tcagcttcta gataaatgca gagatgattt ggaaagtatc ttttctaaga
      961 atcttgtttt aactatcgat gttcttttta atagaaagag tggggcaacc ttaagacata
     1021 aattagcaca tggtaattta tatactaatg cttgttacga tgaaacaacg acatatgcat
     1081 gcatcctgat tttctttctg tgtgcttatc ctctcctacc atattttgat actgtatttg
     1141 aacagggctc tgtttgataa cgtatacgtt gaggcataaa acctatgcat acttagctgc
     1201 atcaatttgc acgaggtatt tcagtatgca ttttctgcgt aatagtaggg attgcttggt
     1261 ttggtagttg taattcaact gcattcaaac cgacccatga agcgggcggg cgaggcgggg
     1321 aaagcactgc gcgctggcgg tggtgctgat tttatttttt cagcgtctga gcgcgtcgtg
     1381 atggcgttta tattgtgcgc cggggcgttg gtgtgtctgc ggggtgtttt gtgcggtggt
     1441 gagcgtgtga gggcgtgatg acggggtgta aaaaagccgc ccgcaggcgg cgatgttcag
     1501 ccgttgtcag tgtccagtga gtagttttta aagcggatga cctcctggcc gagccagccg
     1561 tttatctcgc ggatcctgtc ctgtaacggg ataagctcat tgcggacaaa gacctttgcc
     1621 actttctcaa tatcacccag cgacccgacg ttctccggct tgccgcccat caactgaaag
     1681 gggatgcggt gcgcgtccag caggtcagcg gcgctggctt ttttgatatt aaaaaaatcg
     1741 tccttcgttg ccacttcact gagcgggata attttaatgc cgtcggcttt cccttgcggg
     1801 gcatagagaa acagattttt aaagttgttg cggcctttcg acttgaccat gttttcgcga
     1861 agcatttcga tatcgttgcg atcctgcacg gcatcggtga cgtacatgat gtatccggca
     1921 tgtgcgccgt tttcgtaata cttgcggcgg aacagcgtgg ccgactcatt cagccaggca
     1981 gagttaaggg cgctgagata ttccggcagg ccgtacagct cctgattaat atccggctcc
     2041 agcaggtgaa acacggagcc gggcgcgaag gctgtcggct cgttgaagga cggcacccac
     2101 cagtaaacat cctcttccac accacggcgg gtatattttg ccggtgaggt ttccagcctg
     2161 ataactttac cggtggtgct gtaacgcttt tccagaaacg cattaccgaa caccagaaaa
     2221 tccagcacaa agcggctgaa atcctgctgg gaaagccacg gatgcgggat aaatgtcgag
     2281 gccagaatat tgcgtttgac gtaaatcggt gagctgtgat gcacggcagc acgcaggctt
     2341 tttgccagac cggtaaagct gaccggcggc tcataccatc tgccgttact gatgcactcg
     2401 acgtaatcca gaatgtcacg gcggtcgagt accggtaccg gctcaccaaa ggtgaatgcc
     2461 tccattttcg gggcgctggc ggtcattgtt tttgccgcag gttgcggtgt tttccctttt
     2521 ttcttgctca tcagtaaaac tccagaatgg tggatgtcag cggagtgctg ataccggcgg
     2581 tgagtggctc atttaacagg gcgtgcatgg tcgcccaggc gaggtcggcg tggctggctt
     2641 cctcgctgcg gctggcctca taggtggcgc tgcgtccgct gctggtcatg gtcttgcgga
     2701 tagccataaa cgagctggtg atgtcggtgg cgctgacgtc atattccaga cagcctcggc
     2761 ggataacgtc ttttgccttg agcaccattg cggttttcat ttccggcgtg tagcggatat
     2821 cgcgcgcggc gggatagaac gagcgcacga gctggaacac gccgacaccg aggccggtgg
     2881 catcaatacc gatgtattca acgttatatt tttcggtgag tttgcggatg gattcagcct
     2941 gggtggcaaa gtccatgcct ttccactggt gacgctcaag tattctgaat ttgccaccgg
     3001 ccaccaccgg cggtgccagc accacgcatc cggcgctgtc gccacggtgt gacgggtcgt
     3061 aaccaatcca taccgggcgg gagccgaacg gatttgcggc aaacggcgca tagtcttccc
     3121 attcttccag cgtgtcgacc atgcagcgtt gcagctcctc gaacgggaac accgacgcct
     3181 tgtcgtcaac aaattcacac atgaacaggt ttttaaaatc gtcggcgctg ttttcacgtt
     3241 tgagctgctc aatgtcgaac agcgtgcagc cacctttcag ggcgtcctca atggtgacaa
     3301 tctgccgcca ctggccgtcc gcacagagaa gcccaccggc aagggcgtta tgactgacgt
     3361 cgatttccac gcgttcggcg gcgctggcgc gtccccggtt gaacagttca cctgaccaga
     3421 acgggtaggc gtcgtgcgcc agcgtggacg gggtggagaa ataggtcgaa cgcaggtgac
     3481 tctgtgaggc catacctgat gccaccttac gcagtacctg aaaattcggg atccagaaaa
     3541 tctcgtcgac gtacaggtcg ccgttatggc tctgtgcggt gttggagttg gtgccgagaa
     3601 aaatcagttt tgcgccgtta ttgcccagga caatcgggtc accggtcagg tcaacgtcaa
     3661 ccagacgggc aaaggcgatg atgtattcgc ggaacacata cgcctgcgtt ttactggccg
     3721 acagaaaaat ctggttatga ccggttttca gggcgcgcag cagcgcctcg cgggaaaaat
     3781 aaaacgtcgc gccaatctgg cgggatttca ggatatcgcg gatgcggtgc tcaagcccgg
     3841 cgcgatacca gtgcaactga tattcgaaag actgctcaaa gaaaatctgc tccagctttt
     3901 cgatggcctc gtcactgaaa aaattctttt tcggtttgcg ccgcccgcct ttgttgcggt
     3961 tagcgacgtt cggattaagg tctgcctcgt tgccggtctg gctgtagcgg ttgacccgtg
     4021 ccagtcgttc aatctggcgt ccgagcaggt caatttcctt gaagtcaccg ccggttttct
     4081 gcggtttgat gatgagctgg gtcagccgcg cttccagact catttcgaca cggctgatgg
     4141 gggcaacgct gtcccagccg tcgcgctgtt tccagctctg caccgtcggg cgtttcatct
     4201 gcaacatggc ggcaatctgc ggcacggaaa atccctgcca gtacagcagc gccgcctgac
     4261 gacgcgggtc gtgtaaaaga gtggtgtctg tggtgatggt catgaatacc tcgccgtgat
     4321 gaatacacgg caaggctact gagtcgcgcc ccgcgattcg ctaaggtgct gttgtgtcag
     4381 tgataagcca tccgggactg atggcggagg atgcgcatcg tcgggaaact gatgccgaca
     4441 tgtgactcct ctaatcacta ttcaggactc ctgacaatgg caaaaaaagt ctcaaaattc
     4501 tttcgtatcg gcgttgaggg tgacacctgt gacgggcgtg tcatcagtgc gcaggatatt
     4561 caggaaatgg ccgaaacctt tgacccgcga gtctatggtt gccgcattaa cctggaacat
     4621 ctgcgcggca tcctgcctga cggtattttt aagcgttatg gcgatgtggc cgaactgaag
     4681 gccgaaaaga ttgacgatga ttcggcgctg aaaggcaaat gggcgctgtt tgcgaaaatc
     4741 accccgaccg atgaccttat cgcgatgaac aaggccgcgc agaaggtcta cacctcaatg
     4801 gaaattcagc cgaactttgc caacaccggc aaatgttatc tggtggggct ggccgtcacc
     4861 gatgacccgg caagcctcgg cacggaatac ctggaattct gccgcacggc aaaacacaac
     4921 cctctgaacc gcttcaaatt aagccctgaa aacctgattt cagtggcaac gcccgttgag
     4981 ctggaatttg aagacctgcc tgaaaccgtg ttcaccgccc tgaccgaaaa ggtgaaatcc
     5041 atttttggcc gcaaacaggc cagcgatgac gcccgtctga atgacgtgca tgaagcggtg
     5101 accgctgttg ctgaacatgt gcaggaaaaa ctgagcgcca ctgagcagcg ccttgctgag
     5161 atggaaaccg ccttttctgc acttaagcag gaggtgactg acagggcgga tgaaaccagc
     5221 caggcattca cccgcctgaa aaacagcctc gaccacaccg aaagtctgac ccagcagcgc
     5281 cgcagcaagg ccaccggcgg tggcggtgac gccctgatga cgaactgctg accggcgtca
     5341 gtcagtccgg gaaaaccttc acgattaacc cttaatttca ggaaaaacta tgcgccagga
     5401 aacccgcttt aaatttaatg cctatctgtc ccgtgttgcc gaactgaacg gcatcgacgc
     5461 aggtgatgtg tcgaaaaaat tcaccgttga accgtcggtc acccagaccc tgatgaacac
     5521 catgcaggag tcctctgatt ttctgacccg catcaacatt gtgccggtca gcgaaatgaa
     5581 aggggaaaaa attggcatcg gtgtcaccgg ctccattgcc agcaccagcg acaccgccgg
     5641 tggcaccgag cgccagccga aggacttctc gaagctggcg tccaacaagt acgaatgcga
     5701 ccagattaac ttcgattttt atatccgcta caaaacgctt gacctgtggg cgcgttatca
     5761 ggatttccag ctccgtatcc gtaacgccat tatcaaacgc cagtcccttg atttcatcat
     5821 ggccggtttt aacggcgtga agcgtgccga aacctctgac cgcagcagca atccgatgct
     5881 gcaggatgtg gcggtcggct ggctgcagaa ataccgcaat gaagccccgg cgcgcgtgat
     5941 gagcaaggtc actgacgagg aaggccgcac gacctctgag gttatccgcg tgggtaaggg
     6001 cggtgattat gccagccttg atgcactggt gatggatgcg accaacaacc tgattgaacc
     6061 gtggtatcag gaagaccctg accttgtggt gattgtggga cgtcagctac tggcggacaa
     6121 gtatttcccc atcgttaaca aggagcagga taacagcgaa atgctggccg ctgacgtcat
     6181 tatcagccag aaacgcatcg gtaacctgcc ggcggtacgc gtcccgtact ttccggcgga
     6241 tgcgatgctc atcacgaagc tggaaaacct gtccatctac tacatggatg acagccatcg
     6301 ccgcgtgatt gtggaaaacc cgaaactcga ccgcgtggag aactacgagt caatgaacat
     6361 tgattacgtg gtggaagact acgccgccgg ttgtctggtg gaaaaaatta aggtcggtga
     6421 tttctccaca ccggctaagg cgaccgcaga gccgggagcg taaccgatga cgagtcccgc
     6481 acagcgccac atgatgcggg tctcggcagc gatgaccgcg cagcgggaag ccgccccgct
     6541 gcgacatgca actgtctatg agcagatgct ggtcaagctg gccgcagacc agcgcacact
     6601 gaaagcgatt tattcaaaag agcttaaggc cgcgaaaaag cgcgagctgc tgccgttctg
     6661 gttgccgtgg gtgaacggtg tgctggagca gggcaaaggt gcacaggatg acattctgat
     6721 gacggtcatg ctgtggcgtc tggataccgg cgatattgcc ggtgcgctgg agattgcccg
     6781 ttatgccctg aaatacggtc tgaccatgcc gggtaaacac cgccgcaccc cgccgtacat
     6841 gttcaccgag gaggtggcac ttgcggccat gcgcgcccac gctgccggtg agtctgtgga
     6901 tacccgcctg ctgacggaca ccctcgaact gaccgccacg gctgacatgc ctgatgaagt
     6961 gcgcgcaaag ctgcacaaaa tcaccggtct gtttctgcgt gacgctggtg atgccgccgg
     7021 tgcgctggct cacctgcaac gtgcgacaca gctcgactgt caggcaggcg tcaaaaaaga
     7081 gattgaacga ctggagcggg agctgaaacc gaagccggag tcgcagccca aagcggccac
     7141 ccgcgccccg cgtaagaccc ggagcgtgac accggcaaaa cgtggacgcc cgaaaaagaa
     7201 agccagttaa caaccgaatg cgccccgcgc cagggcggca cgccggtcag tgagggtgaa
     7261 tcacctgaca ctgcaccggc gtccaccgcc cgacttttca gaggtagtca tgatgacgct
     7321 gattattccg cgaaaggagg ctcccgtgtc cggtgagggt acggtggtca tcccgcaacc
     7381 ggcaggcgac gagccggtga ttaaaaacac gttctttttt cccgatatcg acccgaagcg
     7441 cgtccgggaa cgtatgcgcc ttgagcagac cgtcgccccc gcccgtctgc gagaggccat
     7501 caagtcaggc atggcggaga cgaatgcgga gctgtacgag taccgcgaac agaaaattgc
     7561 cgccggtttt acgcgtctgg ctgacgtccc ggcggacgat atcgacggtg aaagcatcaa
     7621 agttttttac tacgagcgcg ccgtgtgtgc gatggcgacc gcgtcgcttt atgagcgtta
     7681 tcgcggcgtg gatgccagtg cgaaaggaga caagaaggcc gacagcattg acagcaccat
     7741 tgatgagctg tggcgggata tgcgctgggc ggtggcgcgt atccaggaca agccgcgctg
     7801 catcgtgagt caaatctgat gaagaccttt gcgctacagg gcgacacgct cgacgccatt
     7861 tgtgtccggt attacgggcg cactgagggc gtggtcgaag ccgtgctcgc cgcaaatccg
     7921 ggactggctg aactgggtgc ggtgctgcca catggcaccg ccgtcgaact gcccgacgtt
     7981 cagaccgcgc ccgtggctga aactgtcaat ctgtgggagt aacgcatgac agcagaagaa
     8041 aaaagcgtcc tgtcgctttt catgattgga gtgctgattg ttgtcggcaa ggtgcttgcc
     8101 ggtggtgaac ccatcacccc gcgtctgttt atcgggcgca tgttgctcgg tggttttgtc
     8161 tcgatggttg ccggtgttgt tctggtgcag tttcctgacc tgtcactgcc tgcggtgtgc
     8221 ggcatcggct ccatgctggg tatcgccggt tatcaggtga ttgagattgc cattcagcgc
     8281 cgctttaagg gcagggggaa accgtaatgc cggtaattaa cacgcaccag aatatcgccg
     8341 cctttctcga catgctggcc gtgtccgaag ggacggcgaa tcatccgctg acgaaaaacc
     8401 ggggctatga cgtgatagtc accggactgg acggaaagcc ggaaattttc accgactaca
     8461 gtgaccaccc gttcgcacat ggccgaccgg cgaaggtgtt taaccgtcgc ggtgaaaaat
     8521 ccacggcctc cggtcgctat cagcagcttt acctgttctg gccgcattac cgcaaacagc
     8581 ttgccctgcc ggatttcagt ccgttgtcac aggacagact cgccattcag ttgatccgcg
     8641 aacgcggtgc gctggatgac atccgggcgg gacgcattga gcgcgccatt tcacgctgtc
     8701 gcaatatctg ggcgtccctg ccgggtgccg gttacggtca gcgtgagcat tcactggaaa
     8761 aactggtcac cgtctggcgt accgctggcg gcgtaccggc ttaaacggag taaacaccat
     8821 gaagaaatta tccctttcac tgatgcggaa cgtgtcgctg gcgctgatgc tggcactgtc
     8881 cctgatttac ccgcagagcg tggccgtcaa ttttgtcgcc gcctgggcga ttctggcgac
     8941 ggttatctgt gtggttgccg gtggtttcgg cgtgtatgcc acggagtatg tgctggaacg
     9001 ctacgggcgg gagctgccgc cggaatcgct ggccgtgaag attgtcgcgt cgctgttttt
     9061 gcagccggtg ccgtggcgcg cacgggcggc ggctctggta gtgatggtgg cgacgtttat
     9121 ctcgctggtc gctgccgggt ggatttttac cgcgctgatt tatcttgtgg cgtcgctgtt
     9181 tttccggctg atacgtaaag cctgtcgtca gcgtcttgag gggcgggaac tatgtcaaag
     9241 ctgatgattg tgctggtcgt gttgttatcg ctggcggtgg ccggtctgtt tctggtgaaa
     9301 cacaaaaatg ccagcctgcg cgcctcgctg gacagggcga acagcgtcgc cagtgaacag
     9361 cagacgacca tcaccatgct gaaaaatcag cttcatgttg ccctcaccag ggcagacaaa
     9421 aacgagctgg cgcaggtggc actgcgtcag gcgctggaaa acgccgcgaa gcgtgaagca
     9481 cagcgcgaga aaaccatcac gaggttactg aatgaaaacg aagattttcg ccgctggtac
     9541 ggcgctggcc tgcctgatgc tgtgcgccgg ttgcaccagc gtccggcctg caccgacgcc
     9601 agtgattgtc cacaacgcct gcccgaaagt gagcctttgc ccgatgccgg gcagtgaccc
     9661 ggagacgaac ggcgatttaa gtgccgatat ccggcagctt gagaacgcgc tggcacgctg
     9721 tgccagccag gtaaaaatga ttaaacactg tcaggacgaa aacgatgctc aaacccgaca
     9781 gcctgcgcag ggcgctgact gatgccgtca cggtgctgaa aaccagtccc gagatgctgc
     9841 ggatattcgt ggataacggg agtattgcct ccacactggc gacgtcgttg tcattcgaaa
     9901 agcgttacac gctcaatgtg attgtgaccg actttaccgg tgattttgac ctgctcatcg
     9961 tgccggtgct ggcgtggctg cgggaaaatc agcccgacat catgaccacc gacgcaggcc
    10021 agaaaaaggg cttcacgttt tatgcagaca tcaacaatga cagcagcttt gatatcagca
    10081 tcagcctgat gctgaccgag cgcacgctgg tcagtgaggt ggacggcgcg ctgcatgtga
    10141 agaatatccc ggaacccccg ccgccggagc cggtcacccg cccgatggag ctttatatca
    10201 atggcgaact ggtgagcaag tgggatgaat gagtttaagc gttttgaaga ccggctgacc
    10261 ggacttattg agtcgctgtc accgtcaggg cgtcggcgac tgagtgccga actggcgaaa
    10321 cgtctgcggc agagtcagca gcgccgggtg atggcacaga aagccccgga cggcacaccc
    10381 tacgcaccac gccagcagca gagcgccaga aaaaagaccg gtcgcgttaa gcgaaaaatg
    10441 tttgcgaaac ttatcaccag tcgttttttg catatccgcg ccagcccgga acaggcatca
    10501 atggagtttt acggcgggaa gtcaccgaaa attgccagcg tgcatcagtt cggtctgtcg
    10561 gaagaaaacc ggaaagacgg taagaaaatt gattatccgg cgcgtcctct gctcggcttt
    10621 accggtgagg atgtgcagat gattgaagag attatcctgg ctcacctcga ccgttagttg
    10681 tgccattccc gacacctcat cgtcacattg ccgccggtat gacccggcgg catccttccc
    10741 gttatgaaca ctctcgcaaa tattcaggaa ctcgcgcgcg cactgcgcaa catgatccgc
    10801 accggcatta tcgtcgaaac cgaccttaac gccggtcgct gccgtgtgca gaccggcggc
    10861 atgtgcaccg actggcttca gtggctgacc catcgcgccg gacgttcgcg cacgtggtgg
    10921 gcaccttccg agggggaaca ggtgctgatt ctggccgtgg gcggtgaact cgacacggcg
    10981 ttcgttctgc cggggattta ttccggcgat aaccccgcgc cgtctgcgtc ggctgatgcc
    11041 ctgcatatcc gtttccctga cggggcggtg attgagtatg aacccgaaac cagtgcgctt
    11101 acggtaagcg gaattaaaac ggccagcgtg acggcttctg attctgttac tgccacggtg
    11161 ccggtggtca tggtgaaagc atcaacccgc atcactctgg acacgccgga ggtggtctgc
    11221 acaaacaagc tgatcaccgg cacgctggaa gtgcagaagg gcgggacgat gcgcggcaac
    11281 attgaacaca ccggcggtga actctcatca aacggtaagg tactgcatac ccataaacac
    11341 cccggcgaca gcggcggcac aaccgggagt cctttatgac agcgcgttat ctcggaatga
    11401 atcgcagtga tggcctgact gtcactgacc ttgagcatat cagccagagt atcggcgata
    11461 tcctgcgcac accggtcggc tcacgggtga tgcgtcgtga ttacggctcg ttgctggcgt
    11521 caatgattga ccagccgcag accccggcgc ttgagttgca gattaaggtc gcctgttaca
    11581 tggcggtgct gaaatgggaa ccccgcgtca ccctgtcatc cgtcaccacg gcgcgcagct
    11641 ttgacgggcg aatgacggtc acgttaaccg gccagcacaa cgacaccggc cagccacttt
    11701 cgttaaccat ccctgtgagt tgaaaccatg ccgattatcg acctgaacca gctacccgca
    11761 ccggatgtgg tcgaggagct ggactttgaa accattctcg ccgaacgcaa ggcgacactg
    11821 atttcccttt acccggaaga ccagcaggag gcggtcgccc gtaccctgac gctggaatcc
    11881 gagcctctcg tcaaactgct ggaggaaaat gcttatcgtg agcttatctg gcgtcagcgt
    11941 gtgaatgagg ccgcacgggc ggtaatgctg gcctgtgccg ccggtaatga ccttgatgtg
    12001 attggtgcca attacaacac cacgcgcctg attatcaccc cggcagatga ttcgaccatc
    12061 ccgccgacac cggcagtgat ggagtctgac accgattatc gtctgcgtat tcagcaggcg
    12121 tttgagggct taagcgtcgc cgggtcggtg ggtgcctatc agtatcatgg tcgcagtgcc
    12181 gacgggcgcg tcgcggatat ctctgtcacc agtccgtctc cggcctgcgt caccatctct
    12241 gtgctgtcac gtgaaaataa cggtgttgca tccgaagacc tgctggccgt ggtgcgtaac
    12301 gcccttaatg gtgaggacgt caggccggtg gccgaccgcg tgaccgtgca gtctgccgcc
    12361 atcgttgaat accagataaa cgccacgctt tacctttacc ctggtcccga aagcgaaccc
    12421 atccgcgctg ctgccgtgaa aaaactggaa gcgtacatca cggcacagca ccggctgggg
    12481 cgcgacatcc gtctgtctgc catttatgcc gctttgcatg tggaaggcgt gcagcgtgtc
    12541 gagctggccg caccactggc cgacattgtg ctcaacagta cgcaggcgtc tttctgcacc
    12601 gaatacagcg tcgtgaccgg aggctcggat gagtgattcg cgactgctgc cgaccggctc
    12661 atcaccgctt gaagtcgctg ccgcaaaagc ctgtgcggaa attgaaaaaa cgccggtcag
    12721 tattcgtgag ctgtggaacc cggacacctg tccggcaaat ctgctgccgt ggctggcgtg
    12781 ggcgttttcg gtcgacaggt gggatgaaaa gtggccggaa gcgacaaaac gcgccgttat
    12841 ccgcgatgcc tatttcattc actgccataa gggcactata ggcgcaatcc ggcgtgtggt
    12901 ggagccgctc ggctatctca ttaacgtaaa ggaatggtgg gagacaaacg acccgcccgg
    12961 aacctttcgc cttgatatcg gtgtgctgga aagcggcatc acggaggaga tgtatctgga
    13021 aatggaacgg cttattgccg atgccaaacc cgcaagtcgc caccttatcg gtctgaacat
    13081 tatccaggac attcccggct atctgtatac aggcggtgtg gtctgtgatg gtgatgttat
    13141 tactgtttat cccggataag tgagaaacaa tgagcacgaa atttaaaacc gttatcacta
    13201 ctgccggagc cgcgaagctg gcagccgcca ctgttcccgg cgggaaaaaa gtaaacctgt
    13261 ctgcaatggc tgtgggtgac ggtaatggcc aattgccggt gccggatgcc ggtcagacga
    13321 aactggtgca tgaagtctgg cgtcatgccc tgaataaagt cagtgtggat aacaagaata
    13381 aaaactatat cgtggctgaa ctggttgttc cgccagatgt gggcggcttc tggatgcgtg
    13441 agcttggtct gtatgacgat gccggaacac tgattgcggt atccaacatg gcagaaagct
    13501 ataagccaga actggctgaa ggctccggac gtgcgcagac ctgccgcatg gttattattc
    13561 tcagcaacgt ggcgtccgtt gagctgagta ttgatgccag cacagtgatg gcgacgcagg
    13621 attacgtcga tgacaaaatc gcagagcatg agcagtcccg ccgccatcct gacgccacgc
    13681 tgacagaaaa aggttttact cagttaagca gtgcaacaaa cagcaccagt gagtcattgg
    13741 cggcaacgcc aaaagcggtc aaggcagcaa atgacaacgc aaattcacgt ctggcgaaaa
    13801 atcagaatgg tgcagatatc caggataaat cagcttttct ggacaatgtt ggcgttacca
    13861 gcctgacgtt tatgaaaaac aacggcgaaa tgccggttga tgctgatctg aatacatttg
    13921 gtcccgttaa ggcttatctg gggatctggt ctaaagctac ctcaactaac gcaacactgg
    13981 agaaaaattt cccggaagat aatgctgtcg gtgtgcttga ggtttttgct gccggcaatt
    14041 ttgcaggcat gcaacgcttt atcacgagag acggcaatgt atacatgcgt aaactcgcca
    14101 ataagtggaa tggtactgat ggtccatggg gcgtatggcg tcacactcaa tcagctaccc
    14161 gccctttgag tacgactata gacctgaata cgcttggagc cgccgaacat cttggtttat
    14221 ggcgtaacag tagctcggct atagcttcat atgaacgcaa ttatccagag gaaggcggct
    14281 ttgctcaggg gacgcttgag atcctcgaag gcgggaatta tggaagaacg caacgttata
    14341 ccactcgccg tggaaatatg tatgtccgct gccttgcggc aagctgggat gcatcaaatc
    14401 cgcagtggga accgtggtta agagtcggtc atcagtcaga gagtcgttat tacgacgggg
    14461 atttgaatga tgtgacttca ccaggtattt acagcgttac aggtaaagcg accaacggcc
    14521 caatactgga cggaaacggc gtgacagtcc tcggtattct ggaagtgttg aggcgctttg
    14581 atggtgttaa cgtatggcag cgttatacaa ctgccggaac aggtgcaacc cttaaaggtc
    14641 gcacgtttga gcgcgtctat accggcagct cgtggagcga atggcgggaa gtctacacct
    14701 cgtattcact tcccctgaat ctgggtatcg gcggtgcagt ggcaaagctc accagcctgg
    14761 actggcagac ccacgacttt gtgccgggca gcctgacaac cgttcggctt gataacatga
    14821 ccaacattcc cgacggtatg gactggggcg tcattgatgg caacctgata aacatcgcag
    14881 ttggtccgag tgatgattcc ggtacggggc gctcaatgca tgtatggcgc agcactgtaa
    14941 gtaaagcgaa ttaccgcttt tttatggtgc gtatttcagg aaatccggga agccgcacga
    15001 tcacgacaag acgtgtgcca attatcgacg aagctcagac atgggcggcg aaacaaacct
    15061 tcagcgccgg tatttcaggc gaactgtccg gcaatgcggc tacagctaca aagctgaaaa
    15121 cgccaaggac aattaacggc gtaaaatttg acggctcggc aaatattgaa gcgtttccgc
    15181 caggtgttcc gctgccgtgg ccatcagata caccacctgc aggctatgcg atcatgcagg
    15241 ggcagacgtt tgataaggct gcatatccga aactggctat tgcctatcct tctggtgtta
    15301 ttcctgatat gcgcggctgg acaatcaagg gcaaacccgc cagtgggcgt gccgtattgt
    15361 cacaggaaca ggacgggatt aaatcgcata ctcacagcgc cagtgcgtca aatacggact
    15421 taggaacgaa aacgaccagt tcatttgatt acgggacaaa aacagtcagt acgtttaacc
    15481 acggcacaaa atcaaccaat acttctggca atcatacaca taccgtcggt tttgctgtga
    15541 gcgtgcaatc aggcggtgct gattatggtg ttccgcgaag tgataaaggt gctacgacta
    15601 caagttcatc aggcaatcat gcacatacag tcgacattgg agcacataac catactgttg
    15661 gtattggtgc acattcacac actgttgcta ttggctcaca cgggcattcc attaccgtaa
    15721 atgctactgg taataccgaa aacaccgtca aaaacatcgc atttaactat attgtgaggc
    15781 ttgcataatg gctttcagaa tgagtgcaga agcacaaact atccgcgttt tcaatttact
    15841 tgatggaacc aatgagttta ttggcgaaag tgatgcatat attccgccgc atacaggtct
    15901 gcctgcaaac agtacagata ttgcaccacc tgatattcct gctggttatg cagccgtttt
    15961 caatgcagat gaaatgaaat gggaactgat ggaagaccat cgcggaaaga ccgtctatga
    16021 aacgaaaacg ggagtagcca tttatatttc tgaacttggc gtattacctc cagacgtgac
    16081 agccatttcc ccggatgggg attatcagaa atggaacgga aatgcgtggg tgaatgatga
    16141 gaatgcagag cgtgatgcgc ttgtcagagc ggctgactct cagaagaaag agctgattgc
    16201 atatgcaggt gaaattattg ccacgctgca ggatgctgtc gatttagata tggctaccga
    16261 ggaagaaaag ttaagcctga cacactggaa aaaataccgt gtgctactga atcgcgttca
    16321 gccggaagat gctccggata tagaatggcc agaaatgccg caataaatcg tattagctct
    16381 ggtatgagat tactcatcta tggcacagag taaaacctaa tctgacagtc cgctctgtgc
    16441 ctgtagcgga agtattgtgc ttgcgacagc tatggaaaac gcactgcgaa cggtgaatca
    16501 ggagagaaaa gattatctat tcttaagctc ggatcactac ggcgtccgtc gtacggtgtg
    16561 gtcggggcat cttaatagca tcgacgcaga acgttatttg cgcaatgtac ggtcggtaat
    16621 cgcgatcatc caatcatttg cgtaaaccaa ctcttgcatt ggtgtaccat cctgcatggg
    16681 gaataatctc cgagtcatgc atgctgtcaa tacggctctc gcgggactct tgctgagaaa
    16741 ctcaacccat cttcactaaa aatgcagtat cctgcgaagt tcttatttct aattcacttg
    16801 ataaaaatct ttatagttct cctttccgta aaacttcctg cgttatttct tcttttcagc
    16861 cccatcaata catcgagaag gcatgcgaag cattaaaaaa actctctaaa tttccgaaaa
    16921 atagaaaatt tgcttggtat aatcgagatt gagatacaca tgttctcata ccccctgccg
    16981 ggcaagcacg gtagggatta ttttcaatca atatggagag gagcctatgg cttaatctgt
    17041 ttatagaagg agtagtaaaa atgaccaaat ggacatttga aaaaaatatt gttgcacaag
    17101 gaattgctgc aaaaaataaa tctggacgta ttccggcgat tgacattcaa ccaggctctg
    17161 gtcttttgaa gcgtcatcaa atggcgttgt atgaaagcgc aatcaacttc agagcgccaa
    17221 ttgaggaaaa taaatgtggg cgatacaacc ccccaggggg atgtgtggcg gtatggtatg
    17281 cctctgactc tgcgttaacg gcggcggcag aagcttatgg gcgtttgctc catctcgaag
    17341 ggacgatttc ttatcctgaa tcggtacttt cacaacacta tatgtgctct gtggatgtca
    17401 tgaggccagt gaaagtgatc gatgttgtag cgctctgtga gttgttacat attcctttgg
    17461 atagcttaga gaacgaggat tacactttta ctcaatggct aatggaacat ctttatacac
    17521 atttcgggcg tgactatgac gggatagcct atacctcaag gcataaacgc tataaacgct
    17581 gttatgcctt ttgggaacga ccggatagta caccagcctt taccgatacc cctcagggga
    17641 tggtgcctta tgccagttac atcgaaaatg acgaaggtat cttccctaaa gggtggcaca
    17701 aaggaacgat gtcaggtgaa gagatgttcg aagaactgct tggattcgaa attacgccag
    17761 aaccaatgta aaccaaagag gggctggcta gcccctcttt ttaaaacaat taactggata
    17821 catgatttcc gaactgttta gcggcacgga tagccagact aagttcacta tttttagcgc
    17881 cgtcctgcat gatttgaatc ggcgtctttt tcaggccttc gctattaacc tctattggtg
    17941 atttcagcac acccaacctc agcattggat gggtattctc gtcaaatgcc gccattacgt
    18001 cttcaaagcc gggaatcagc ccgttatctg tgaactgaaa tttcgggaaa ataaaatcac
    18061 cgttctgctt gaatgctaaa agcttgttct tcttaacgcg aatgttgatc gcctggcggg
    18121 tgattccaag aatatcgacc acgtcattta cctttagcgt accgcctaac tcctgaagcc
    18181 cacgataaaa tcgttccaga ttttcgcgac gacggcgcac tttacgtagt tcgtagtcat
    18241 tgacacgagt cttctgttcg agttgaacgg tgtagagcaa ttgcatcagt gaactatcat
    18301 ccagcgtgat agcattaatt gcctggtttt tgtcttcaaa ttcaggcatc agggaaatag
    18361 cgaggttgtt aattcttttc agaaggttca actggtattc attcagctta gctgaagtgt
    18421 tggtttccct ttctgctgcg gtgtgtttga tcattgtgca cctccatttg acgattatca
    18481 taattcattt tattggaatg gtcaaattca agggcaagca atctatggtc aatcaaggcg
    18541 taattcgttg gcatagaccc gattgactca tgcaccgtga tgttagcatg cacataatta
    18601 tggttatcca cacctcgtac aaatcagtct gccagacttg aaagcttttt ggctacgtaa
    18661 attgtcagtt ggaagctggg ttagtacaaa ttaggaaatg cgggcgaatt gcccgccttt
    18721 tctgtatctg ttgtttcatc cactgaccat ccagggcaaa tagcgtctca tgctctgcac
    18781 aacagaaaat agttgcaccc attaaccacg gagttaaacg gatgagtgac tatcatcatg
    18841 gcgtgcaggt gctggagatt aacgacggca cccgcgtcat ttccaccgta tccacggcca
    18901 ttgtcggcat ggtctgcacg gccagcgatg cggatgcgga aaccttcccc ctcaataaac
    18961 cggtgctgat taccaatgtg cagagtgcaa ttgcgaaggc cggtaaaaaa ggcacgctgg
    19021 cggcatcgtt gcaggccatc gccgaccagt caaaaccggt caccgttgtc gtgcgtgtgg
    19081 aagacggcac cggcgacgac gaggaaacga aactcgcgca gaccgtttcc aatatcatcg
    19141 gcaccactga cgaaaacggt cagtacaccg gactgaaagc cctgatgggg gctgagtcgg
    19201 ttaccggcgt taaaccgcgc attctcggtg tgccggggct ggacaccaaa gaggttgccg
    19261 tcgcactggc atcggtatgc caggaactga atgcattcgg gtatatcagc gcatggggct
    19321 gtaaaaccat ttccgaggtg aaagcctacc gccagaattt cagccagcgt gagctgatgg
    19381 ttatctggcc ggatttcctc gcatgggata cggtcaccag taccaccgcc accgcgtatg
    19441 ccaccgcccg tgcgctgggg ctgcgtgcca aaatcgacca ggagcagggc tggcataaaa
    19501 cgctgtccaa cgtcggggta aacggtgtta ccggcatcag tgcatctgta ttctgggatt
    19561 tgcagaagtc tggcactgat gctgacctgc tgaacgaggc aggcgtcaca acgctggttc
    19621 gccgagacgg tttccgtttc tggggtaacc gtacctgctc cgatgacccg ctgttcctct
    19681 ttgaaagcta cacacgcact gcgcaggtac tggccgacac gatggttgag gcgcacatgt
    19741 gggctattga taagcccatc accgcaacgc tgattcgcga catcattgac ggcatcaatg
    19801 ccaaattccg cgaactgaaa aacaacggtt atatcgtgga tggcacatgc tggttcagtg
    19861 aagaatccaa cgatgcggaa accctcaagg ccggaaaact gtatatcgac tacgactata
    19921 ccccggtgcc tcccctcgaa aacctgaccc tgcgccagcg tattacttca agatacctgg
    19981 caagtctggt cacctcggtt aacagcaatt aaggagcctg accgatggca atgccgcgca
    20041 aactcaagtt aatgaacgtc tttctgaacg gctacagcta tcagggcgtt gcaaagtccg
    20101 tcacgctgcc aaaactgacc cgtaagctcg aaaactatcg cggtgcgggg atgaacggca
    20161 gcgcaccggt agacctcggc cttgatgacg atgcgctgtc aatggagtgg tcgctcggtg
    20221 gcttcccgga ttcggttatc tgggagcttt acgccgcaac cggtgtggat tccgtaccga
    20281 ttcgttttgc aggctcttac cagcgcgacg ataccggcga aacggtggcc gtcgaggtgg
    20341 tcatgcgtgg acgtcagaaa gaaatcgaca ccggcgaggg caaacaggga gaagacaccg
    20401 agtcgaaaat ctccgtggtc tgcacctatt tccggctgac gatggacggt aaggagctgg
    20461 tcgaaatcga caccatcaac atgattgaga aggtgaacgg cgtcgaccgg ctggagcaac
    20521 accgccgcaa tatcggcctg tgattttcat ccggtcagcc tggctgaccg gttaaccccg
    20581 attcagaagt gagaaaacca tgaacaaaga aaatgtcatt accctggaca atccggtcaa
    20641 acgtggtgag caggttatcg aacaggtcac gctgatgaaa cccaatgccg ggacgctgcg
    20701 cggtgtcagt ctggcagcgg tcgcgaactc cgaagtcgat gcactgatta aggtgctgcc
    20761 gcgcatgacg gcaccgatgc tgaccgagca ggaagtcgcc gcgctggaac tgcctgacct
    20821 tgtggcgctg gccggtaagg tggtcggttt tttgtcgccg aactcggtgc agtgacgttc
    20881 ccgaaaaatc tgtcggtcga tgacctgatg gcggatgtgg cagtgatatt tcactggccg
    20941 ccatcagaac tgtatcccat gagcctgacc gaactcatca catggcgcga aaaggcgctc
    21001 cggcgaagcg gaaacacgaa tgagtaacaa tgtaaaatta caggtattgc tcagggctgt
    21061 tgaccaggca tcccgcccgt ttaaatccat ccgcacagcg agtaagtcgc tgtcggggga
    21121 tatccgggaa acacaaaaat cactgcgcga gctgaacggt cacgcatccc gtattgaggg
    21181 atttcgcaag accagcgcac agcttgccgt gactggtcat gcacttgaaa aggcacggca
    21241 ggaagccgaa gcccttgcca cacagtttaa aaacaccgaa cgtccgaccc gtgctcaggc
    21301 gaaagtgctg gaatccgcaa agcgtgcggc ggaggactta caggcgaaat ataaccgcct
    21361 gacggattcc gttaaacgcc agcaacggga actggccgct gtgggaatta atacccgcaa
    21421 tcttgcacat gatgagcagg gactgaaaaa ccgtatcagt gaaaccaccg cacagcttaa
    21481 ccgtcagcgc gacgcgctgg cgcgtgtcag tgcgcaacag gcaaaactta acgcagtcaa
    21541 acagcgttat caggcaggca aggaactggc cggaaatatg gcctcggtgg gcgctgccgg
    21601 tgtggggatt gcggcggcgg gaacgatggc cggagttaag ctgctgatgc ccggttatga
    21661 gtttgcgcag aaaaactcag aattgcaggc cgtgctcgga gtggcaaaag actccgccga
    21721 aatggctgca ctacgcaaac aggcgcgcca gctcggcgac aataccgccg cctcggcaga
    21781 tgatgcagcc ggtgcgcaga ttattattgc gaaagccggt ggggatgttg atgccattca
    21841 ggcggcaacg ccggtcacgc tgaatatggc gctggcgaac cgtcgcacga tggaagaaaa
    21901 cgctgccctg ctgatgggga tgaaatccgc ctttcagctt tcaaacgata aggtcgctca
    21961 tatcggggat gttctctcca tgacgatgaa caaaaccgcc gccgattttg acggtatgag
    22021 cgatgcgctg acctatgctg cacctgtggc aaaaaatgcc ggtgtcagca ttgaagaaac
    22081 cgccgcaatg gtcggagcgc tgcatgatgc aaaaatcaca ggctcaatgg cggggacggg
    22141 aagccgtgcc gtgttaagtc gcctgcaggc accgacggga aaagcatggg atgcactcaa
    22201 agagcttggc gtgaaaacct cagacagtaa gggaaacacc cgaccagtat ttaccattct
    22261 gaaagaaatg caggccagtt ttgagaaaaa ccggctcggt actgcccagc aggctgaata
    22321 catgaaaacc attttcgggg aggaagccag ctcagccgcc accgtgctga tgactgccgc
    22381 ctcaaccgga aagctggaca aactgaccgc tgcgtttaaa gcctcagacg ggaagaccgc
    22441 cgagctggta aatatcatgc aggacaacct cggcggtgac tttaaggagt ttcagtccgc
    22501 ttatgaggcg gtggggactg acctgtttga ccagcaggaa ggcgcgctgc gtaagctcac
    22561 gcagacggcc acaaagtatg tgttaaaact cgacggctgg atccagaaaa acaaatcact
    22621 ggcgtcaacc atcggcatca ttgccggtgg cgcgctggcg ctgactggca tcatcggtgc
    22681 aattggtctt gtagcctggc cggttatcac cggcatcaat gccatcatcg cggcagcagg
    22741 cgcaatgggg gcagtcttca cgacggttgg cagtgctgtt atgaccgcca tcggggctat
    22801 tagctggccg gttgtggccg tggtggccgc aattgtcgcc ggggcgttgc ttatccgtaa
    22861 atactgggag cctgtcagcg cattctttgg cggtgtggtt gaagggctga aagtggcatt
    22921 tgcgccggtg ggggaactgt tcacgccact taagccggtg tttgactggc tgggtgaaaa
    22981 gttacaggcc gcgtggcagt ggtttaaaaa cctgattgcc ccggtcaaag ccactcagga
    23041 caccctgaac agttgccgtg acacgggggt catgttcggg caggcactgg ctgacgcgct
    23101 gatgctgccg cttaatgcgt tcaacaaact gcgcagtggt attgactggg tactggaaaa
    23161 actcggtgtt atcaacaaag agtcagacac acttgaccag accgccgcca gaactcaagc
    23221 cgccacgtat ggcagcggtg gttatattcc ggcgaccagc tcttatgcag gctatcaggc
    23281 ttatcagccg gtcacggcac cggctggtcg ctcttatgta gaccagagta aaaacgaata
    23341 tcacatcagc ctgacgggtg gtactgcgcc ggggacacag cttgaccgcc agttacagga
    23401 tgcgcttgaa aaatacgagc gggataaacg tgcgcgcgcc cgtgccagca tgatgcatga
    23461 cggttaagga ggtgacgaaa aatgatgctc gcgttaggta tgtttgtttt tatgcgccag
    23521 acgctgccac accagaccat gcagcgtgaa tcagattatc gctggccgtc aaattcccgt
    23581 atcggtaaac gggatgcctt tcagtttctc ggtgttggcg aggaaaacat cacgcttgcc
    23641 ggtgtgcttt atcccgagct gaccggcgga aagctgacga tgaccacgct caggctgatg
    23701 gcagaggaag gtcgggcgtg gccgttgctg gatggcaccg gcatgattta cggcatgtat
    23761 gtcatcagca gggtgagtga aacagggagt attttctttg cagacggcac accccgaaaa
    23821 attgatttta cgctgtcgct cacccgcgtt gatgaatcac tggccgcgct ttatggcgat
    23881 atcggtaaac aggcggaatc gctcatcggt aaggctggca gtatggcgac taaattcacg
    23941 ggtatgacgg gggcgggata atgctggatg cgctgacatt tgatgcaggc agtacgctga
    24001 cgccggatta catgctgatg ctcgacagca gggatattac cggcaatatc agcgaccgtc
    24061 tgatgagcat gaccctgacg gataaccggg gctttgaggc tgaccagctt gatattgaac
    24121 tgaacgatgc cgacgggcag gtcgggctgc cggttcgtgg cgctgtcctg acggtgtata
    24181 tcggctggaa aggttttgcc ctggtatgca aagggaaatt taccgttgat gaggttgaac
    24241 accggggcgc gccggatgtg gttaccatcc gcgcccggag tgcagatttt cgcgggacgc
    24301 tcaattcccg ccgggaaggc tcatggcatg acaccacgct cggtgcgatt gttgaggcga
    24361 tagcctcccg taacaggctg gaagccagtg tcgctccgtc actggccgga attaaaatcc
    24421 cgcacatcga ccagtcgcag gagtctgatg caaaattcct gacccgcctt gctgaacgca
    24481 acggcggtga ggtgtcggta aaaatgggaa aattgttgtt tctcaaagcg gggcaggggg
    24541 tgacggccag cggtaaaaaa atcccgcaga ttaccatcac ccgcagcgac ggcgaccgtc
    24601 atcattttgc gattgctgac cgtggagcct acaccggcgt aacggcaaag tggcttcaca
    24661 ccaaagaccc gaagccgcaa aagcagaagg taaaactgaa acgcaaaaag aaagagaaac
    24721 acctgcgcgc actggagcat cccaaagcga aaccggtcag gcagaagaaa gcgcctaaag
    24781 taccggaagc gcgcgaaggt gaatacatgg ccggtgaggc tgacaacgtt tttgcactga
    24841 ccacggtata tgccacgaaa gcgcaggcca tgcgcgccgc tcaggcgaag tgggacaaac
    24901 tgcaacgggg cgtcgcggag ttttccatca gcctggctac cggtcgggca gatatttaca
    24961 cggaaacacc ggttaaagtg tcaggcttta agcgcgtcat agacgagcag gactggacaa
    25021 tcactaaggt gacacatttt ctgaataata gcggcttcac gacgtcctta gagcttgagg
    25081 tcaggctttc tgatgtggag tacgaaa
//
LOCUS       DAJOTB010000063        24870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJOTB010000063 DAJOTB010000000
VERSION     DAJOTB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.063"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            78..4007
                     /locus_tag="OHN28_002494"
     CDS             78..4007
                     /locus_tag="OHN28_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2297882.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLAIFIVSALS
                     YGAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPD
                     FSPVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLD
                     KFVVETAGATEHADFNLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYN
                     PLLRSASMFQLNWNNMYASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKV
                     FSSKDTWAFFARYDQNTVDILKNTFTQEVNLNGQKMTVNNKNIAINDKITAIELTKSN
                     KNKDLKFHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDW
                     SVKGEANDNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLG
                     QADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQN
                     PSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHA
                     IFREGPRHCYVPGVLCDKDYVTDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHF
                     RFKTLNLENSEFTTARNSVVEGDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQ
                     DVREGRSADDGSSSYTGKITLQKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGV
                     FVNSTLSVRDGGHLTAQKGLYSNGRVQIGKKGTLSLSGTPENGADNTWMPVLTYMTEG
                     YDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNA
                     AYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATG
                     SDFVLRTDLKDADKISITEKASGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIF
                     KAGTRVTGFSRVTPTLRVDTTGGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTE
                     VNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLF
                     TGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFA
                     GLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMK
                     NKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGE
                     KDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANFRYMF"
     gene            4221..4493
                     /locus_tag="OHN28_002495"
     CDS             4221..4493
                     /locus_tag="OHN28_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2297883.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            4721..5017
                     /gene="ghoS"
                     /locus_tag="OHN28_002496"
     CDS             4721..5017
                     /gene="ghoS"
                     /locus_tag="OHN28_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2297884.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            5045..5218
                     /gene="ghoT"
                     /locus_tag="OHN28_002497"
     CDS             5045..5218
                     /gene="ghoT"
                     /locus_tag="OHN28_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCQ2297885.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(5337..6854)
                     /gene="lysS"
                     /locus_tag="OHN28_002498"
     CDS             complement(5337..6854)
                     /gene="lysS"
                     /locus_tag="OHN28_002498"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2297886.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7091..8548)
                     /gene="dtpC"
                     /locus_tag="OHN28_002499"
     CDS             complement(7091..8548)
                     /gene="dtpC"
                     /locus_tag="OHN28_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCQ2297887.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(8607..10754)
                     /gene="cadA"
                     /locus_tag="OHN28_002500"
     CDS             complement(8607..10754)
                     /gene="cadA"
                     /locus_tag="OHN28_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCQ2297888.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(10834..12168)
                     /gene="cadB"
                     /locus_tag="OHN28_002501"
     CDS             complement(10834..12168)
                     /gene="cadB"
                     /locus_tag="OHN28_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCQ2297889.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(12534..14072)
                     /gene="cadC"
                     /locus_tag="OHN28_002502"
     CDS             complement(12534..14072)
                     /gene="cadC"
                     /locus_tag="OHN28_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCQ2297890.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(14895..15842)
                     /locus_tag="OHN28_002503"
     CDS             complement(14895..15842)
                     /locus_tag="OHN28_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCQ2297891.1"
                     /translation="MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETH
                     TLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQNGRNRKRKIRKEIVQIITDVLH
                     NSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
                     DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHI
                     YDNYCIVREKGVNVPVLKNFVQICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDP
                     VHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN"
     gene            complement(15835..16230)
                     /locus_tag="OHN28_002504"
     CDS             complement(15835..16230)
                     /locus_tag="OHN28_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCQ2297892.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITEALDRLNIRYSLIGATECYNKGQ
                     STQIPVSPEFKTPPRFKRVLVVGKSKINA"
     gene            complement(16299..17144)
                     /locus_tag="OHN28_002505"
     CDS             complement(16299..17144)
                     /locus_tag="OHN28_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2297893.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVLNVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPN
                     VLTER"
     gene            complement(17229..17426)
                     /locus_tag="OHN28_002506"
     CDS             complement(17229..17426)
                     /locus_tag="OHN28_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCQ2297894.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            complement(17444..17932)
                     /locus_tag="OHN28_002507"
     CDS             complement(17444..17932)
                     /locus_tag="OHN28_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297895.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLIVTLIRHYGVSLIHLDACAECLPGLPTF
                     DW"
     gene            complement(17929..18306)
                     /gene="cbtA"
                     /locus_tag="OHN28_002508"
     CDS             complement(17929..18306)
                     /gene="cbtA"
                     /locus_tag="OHN28_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCQ2297896.1"
                     /translation="MNTLPVLPGQAASSRPSPVEIWQILLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            complement(18396..18479)
                     /locus_tag="OHN28_002509"
                     /pseudo
     CDS             complement(18396..18479)
                     /locus_tag="OHN28_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            19008..20249
                     /gene="ltrA"
                     /locus_tag="OHN28_002510"
     CDS             19008..20249
                     /gene="ltrA"
                     /locus_tag="OHN28_002510"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCQ2297897.1"
                     /translation="MNHHPKPFMISKIAVWKAYQRIRANRGSPGVDGQTIETFEGNLS
                     GNLYKLWNRMASGSYMPPPVRRVEIPKATGGTRPLGIPTVADRIAQMVVKDVLEPILE
                     PHFHNDSYGYRPHKSAHDALRAARHRCWRSNWVLDVDIKGFFDNIDHDLLMKAVCKHT
                     RCKWTELYIRRWLTAPVQMPDGTLHARDRGTPQGGVISPLLANLFLHYAFDLWMTRQY
                     PDRPFERYADDIVIHCNSQAQAIVLRNKLEHRLAECKLELSQSKTKIVYCKDGKRREN
                     YPDISFDFLGYTFRPRKARAKDGVKFLSFMPAISGKASKMIRQTIRGWKWKQRTTLTV
                     EEVARRINPILRGWITYYECFYRSALYPVLAHVDLHFAKWLADKYKRLRGSRKRAFLL
                     LKHIRRKRPGLFVHWCMAYKS"
     gene            complement(20286..20630)
                     /locus_tag="OHN28_002511"
     CDS             complement(20286..20630)
                     /locus_tag="OHN28_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2297898.1"
                     /translation="MSDTLPGTTHPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLA
                     DRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTYRVAQ
                     GNLPPTLPQNRT"
     gene            complement(20704..20925)
                     /locus_tag="OHN28_002512"
     CDS             complement(20704..20925)
                     /locus_tag="OHN28_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2297899.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(20988..21464)
                     /locus_tag="OHN28_002513"
     CDS             complement(20988..21464)
                     /locus_tag="OHN28_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2297900.1"
                     /translation="MQQISFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEITPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(21480..21959)
                     /locus_tag="OHN28_002514"
     CDS             complement(21480..21959)
                     /locus_tag="OHN28_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2297901.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPEYDAIMRIID"
     gene            complement(22041..22859)
                     /locus_tag="OHN28_002515"
     CDS             complement(22041..22859)
                     /locus_tag="OHN28_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2297902.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTAGILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRTIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(22949..23181)
                     /locus_tag="OHN28_002516"
                     /pseudo
     CDS             complement(22949..23181)
                     /locus_tag="OHN28_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(23187..23864)
                     /locus_tag="OHN28_002517"
     CDS             complement(23187..23864)
                     /locus_tag="OHN28_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2297903.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEAGQAPEMV
                     KKAAHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTSVINKDSVLQ
                     AARQAYSREYDEETTETADFDSYEIKGNKVEFEVFNPGYRTYDKVTVTVGADGNATDA
                     SVEFIGK"
     gene            complement(24012..24692)
                     /locus_tag="OHN28_002518"
     CDS             complement(24012..24692)
                     /locus_tag="OHN28_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2297904.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
BASE COUNT         6674 a         5682 c         6146 g         6368 t
ORIGIN      
        1 taattggaag ctttttatta ataaaaacat agttctattg gtttgtatta tgttataaac
       61 gttatggagt cttacccgtg aacagaatat attcacttaa gtattgtcac cttactcaag
      121 ggcttatagc tgtatcagaa ttagcatccc gagtatcctc taaaaccggt cgaaaacttc
      181 tcgctatatt tatagtttct gccttgtctt atggtgcggc tgaatatgcc tgtgcagcac
      241 agatggacac ccgaaatttc tggatccgtg attatcttga tctggcacag aataaaggtg
      301 catttcagcc cggagcatac ggggtaaaaa cgccattaaa aaatggggga gaattcagtt
      361 ttcctgaagt aacaatccct gatttttctc ctgtatccgc taaaggtgca acaactgcta
      421 ttggtaatgc ctacagtgtt acagcaagtc ataatggcac tattcaccat gccattaaaa
      481 ctcagacatg gggacagtca gattatcatt atgttgatcg tgtgaccaaa ggtgactttg
      541 cggtccagcg tctggataag tttgttgttg aaacagcggg tgcaacagag catgctgatt
      601 tcaacttatc agcagcagaa gcactagagc gttatggtat tgaattcaat ggaaagaaac
      661 aaataatcgg tttccgcgtt ggagctggtg caaccggtgt tacatcttat ggggtgggac
      721 agacatacaa tccattatta cgtagtgcct ccatgtttca gttaaactgg aacaatatgt
      781 atgcaagtaa taataccggt gggttttata atgaagtgac aggaggtgac agcggttccg
      841 gattttactt gtatgataat cagaaaaaaa aatgggtcat tttggggaca ctgacaggta
      901 aagtattctc cagtaaggat acctgggcct tttttgctcg atatgatcag aacactgtcg
      961 atatcctgaa aaatactttt acccaagagg tgaacctcaa tggtcagaaa atgacagtta
     1021 ataataaaaa tattgccatt aacgataaaa taactgctat tgaactgacc aagagtaata
     1081 aaaataaaga tttgaaattc catggtggcg ggagcattga gctcaccgat aacctgaact
     1141 caggaaccgg aggattgatt tttgatgagg gacaacatta ttcggttatt gggaaagata
     1201 aagcctataa aggggcgggt gttgagatcg gaaaagatac ggttgtcgac tggtcggtaa
     1261 aaggggaggc aaacgataac ctgcacaaaa caggggccgg gacactgaat gtcaatgtgg
     1321 cccaggggaa taacctgaaa acaggtgacg gtaccgtttt tcttaatgca gaaaaggctt
     1381 tcaatgctat ctatgttgcc agtggccgtg gaacggtcaa actggggcag gccgatgcgc
     1441 tggataaaaa tagtgattac agaggtattt attttactag tcgtgggggg actctggatt
     1501 taaacgggtt cagccagtcg ttcaagaaga tcgcggcaac tgatgttggt accattatca
     1561 ccaatacttc tgataaaaca gcgacccttt ccctacaaaa cccctcccgc tatgtctatc
     1621 acggtagtat cacgggaaat acgaatatcg aacacactgg gacacagaaa agtgctgaca
     1681 gcagtctgat tattgatgga aacattaata cacgcaatga cattactgtg cggaattccc
     1741 agctcagact tcaggggcat gccacatcac atgcaatatt ccgcgagggg cctcggcact
     1801 gctatgtccc cggagttctt tgtgacaaag attatgttac tgattttgcc agactggaaa
     1861 gtgaggcaaa taagaaaaat aacagtgcct ataaaacaaa taatcaggtg gcttcttttg
     1921 accaacctga ctgggaaacc cggcatttcc gatttaagac tctgaatctg gaaaactcag
     1981 aattcacaac tgcacgtaac tcagttgttg agggtgatat tgtcgcatcg aattcaacgc
     2041 tgaaactggg gggcgacgtt ccggtgttca ttgatatgta tgatggcatc aatatcaccg
     2101 gtaatggttt tggcttccgc caggacgttc gtgaaggacg ctcagcagat gatggcagta
     2161 gcagctatac aggcaaaatt acactgcaaa aaggctccac gctggacatc aacaaccggt
     2221 tcattggcgg tattgaagcc catgacagta aggtaaacgt cacctcaccg gatgccctgc
     2281 tgcagaacag tggtgttttc gtgaattcca ccctttctgt ccgtgacggc ggtcatctga
     2341 cggcacaaaa agggctctac agtaacggcc gggttcagat tggaaagaaa ggtacgcttt
     2401 ccctgagcgg cacgccggaa aatggcgctg ataatacctg gatgcccgta ctgacataca
     2461 tgacagaagg ctatgattta accggtgata acgctacgct gaacatcagc cagcaggcgc
     2521 atgtttccgg ggatgttcat gcaaccagtt catccagcat tcgtattggc tccgaaaacc
     2581 ctggctcagt ttcctcttct gtctcccctg ttctggctgc cgggttgttc agcggatata
     2641 acgcggcgta ctacggtgcc atcaccggcg gtaaggggaa cgtcagtatg aataatggcc
     2701 tgtggcagct gaccggagat tccgacatca acagtctgac gacccgtaac agccgggtac
     2761 agtctgaaga aaacggtgcc ttccgtaccc tgacggttaa gacacttgat gccacgggca
     2821 gtgattttgt cctgcgcact gatctgaagg acgctgataa aatcagtatt acggagaaag
     2881 ccagcggttc agacaacacc ctgaatgtca gctttatgaa gaacccgtct ccgggacagt
     2941 ccctgaatat cccgctggtc agtgcaccgg ccggaacatc aggggatatc tttaaggccg
     3001 gcacccgggt gacaggcttc agtcgtgtga cgccgacgct gcgtgttgac accactggcg
     3061 gcagtacgaa gtggattctg gatggtttca ggacggaagc tgataaagcg gctgcagcga
     3121 aggcgaacag tttcatgaat gccggctaca aaagctttat gacggaagta aataatctga
     3181 acaaacgtat gggggagctg cgtgacacga acggtgatgc cggtgcctgg gcccgtatta
     3241 tgaacggcgc cggttcagcc gatggcggat acagtgataa ctacacccac gttcaggtcg
     3301 gttttgacaa aaaacatgcg ctggacggtg ttgacctgtt caccggtgtc acgatgacct
     3361 ataccgacag cagtgcagac agtgatgcgt tcagcgggaa gacaaaatcc gtggggggag
     3421 gtctgtatgc ttcagcactg tttaactccg gtgcctacat tgatttgatt ggtaaatata
     3481 ttcaccatga caatgactac acaggtaact ttgccggtct ggggacgaag cactacggaa
     3541 cccactcctg gtatgccgga gcggaaacgg gttaccgtta tcacctgacg gaagacacat
     3601 ttattgagcc tcaggccgaa ctggtttacg gcgcagtgtc cgggaaaaca ttccgctgga
     3661 aagacggtga gatggacctg agtatgaaga acaaggattt cagcccgttg attggcagaa
     3721 cagggattga gctgggcaaa accttcaggg gtaaggactg gagtgtgaca gcccgtgccg
     3781 gaaccagctg gcagtttgac ctgctgaata atggcgagac agttcttcgt gatgcatccg
     3841 gagagaaacg gatcaaaggg gagaaagaca gcaggatgtt gttcaatgtt ggcatgaacg
     3901 cacagataaa ggacaacatg cgctttggtc tggagtttga gaaatccgcc tttggtaaat
     3961 acaacgtgga taacgcaata aacgcgaatt tccggtatat gttctgacaa ggagaggccc
     4021 ctgaagaggg gcctgtagtg atgttactcc cgggttaatg cagctagtaa aggtatggct
     4081 ctgactaccc tggaaagtgg tgcccatcgc atcactacaa attctggcaa ttgcgtacgt
     4141 ggtaacggca agttatttaa cctcaaacgt acgtgcccga gcggtgcgct gaaataccgt
     4201 cataaataag cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg
     4261 acgaacaggg caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga
     4321 ttatcgaaca taccgatgtc gatgaaagcc tgaaaggaca agggattggt aaacagctgg
     4381 ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat
     4441 ttgcgaaaca cgaatttgat aaaacacggg agtatgatga tattcgcagt tgatgggaga
     4501 gtacagagtc acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg
     4561 gtaggagatg cacaaagaat ggtcagagag agcgtttttt tgtcccaagt catccccttt
     4621 actgagcaaa aaaaaaagaa tatctcctat atgagaatca tcattcgggg ttaataagtt
     4681 ttgcgtcccc agagcgttca atattgatag gagtcatatt atggaaggta aaaacaagtt
     4741 caatacttat gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt
     4801 aagatcattg atgtatgata tgaatttctc ctctatcgtg gctgatgaat atgggatacc
     4861 acgacaattg aatgaaaact ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga
     4921 agatttaatc aggctcaaat gcttagactt accggatatt gattttgacc tcagcattat
     4981 gacagttgat gactatttcc gtcagtttta caagtagcca ggaaggataa gaagaacata
     5041 aaaaatggca ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt
     5101 tgtcattatc tggtttatct cacatgagaa aacacatatt cgtttactta gtgcatttct
     5161 ggtcggaata acctggccaa tgagtttgcc tgtggcatta cttttttctc tcttttagga
     5221 gctgtagttg agtggacccg ctggttatct tgaaacgaag cgtctctcct caaaactgga
     5281 tttgcggaat gacgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat
     5341 ttctgtgggc gcatcgccgg gaagagaata acgtcgcgaa tagtatgact gtttgtaaac
     5401 agcataatca ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc
     5461 agcgcagtca cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc agctttagcg
     5521 ttaacctgtt cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca
     5581 ttaccaattt cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca
     5641 tcattacggc gcgccagcgg ggacacttct gccggatatt ccgtaataaa ggttggctga
     5701 atcagatgtg cttctgccac ttcatcaaag atctccgtaa caatacgccc cagtccccag
     5761 cttttctcta ccgtaatacc gatagattca gctaatgctt tagcagcatc aaaattatcc
     5821 agatccgcca tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt
     5881 tcaaacggtt tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccagt
     5941 acctcttgtg ccagggtgcg gaacagtgac tccgtcagtt caatcaaatc atggtaatcc
     6001 gcatatgcca tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct
     6061 tcattacgga agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt
     6121 ttcagataca gctccggcgc gatacgcagg tacatatcta aatccagagc gttatgatgg
     6181 gtaataaacg ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact
     6241 tccataaagc cgcgcgcgac catgaactga cggatagcgg ccagaatttt tgaacggaca
     6301 acaaacgttt gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg
     6361 acttcctgat cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc
     6421 aggcgcagct cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg
     6481 cgggcaccga taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct
     6541 ggcaggctat ctcttgcaac gtacagttga atacggccac cgacatcctg caacgttaca
     6601 aaggaggctt tccccataat acgacgggtc atcattcggc cagcaaccga gacttcaatg
     6661 tttaaggatt ccagttcctg gttatccttc gcatcaaact cttcgtgcaa ctggtcagag
     6721 gtatggtcgc ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt
     6781 ttttcgcggc gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt
     6841 tctcgttcag acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga
     6901 cagatcggta cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa
     6961 ctaacgctta cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact
     7021 tattaatctc gatttactag tgtgataaac tgaaagttaa tttattcata accaattgtt
     7081 tttacgacag ttaatcgttg ctctcctgta tcatattcgt cggcgtgcta aacagaaaac
     7141 gggtagcaaa ggccaatacc acgatgatcg cgacacaagc caacgtccac tctcccatct
     7201 gagaaaagaa tcgctggtaa gcggcaattg ccataccgct aatttgcgac tctgtcgtct
     7261 gctgtgccac gacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatac
     7321 cggttaatac gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa
     7381 agagttcagc aaagcccatt agcgccagcc cggatatcat cacgcccatt gacgcttgac
     7441 cgccagccgc tgcatgtcgg gcatcaaatg ccagcaacat aaagccacaa gccatcagca
     7501 gtaagccaaa ggcaaacttc agccagacgc gcaatgctga attgccgcgg ctttctggcg
     7561 acgccagcca ggccagaaca accccagcga gcatcaccgc aatagcattc accgactgga
     7621 atagtgctgt aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca
     7681 agctgatggt actgccgccc tgttgtgcca gtacccagaa caacgtcccg acaaacatca
     7741 acaatacaat ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa
     7801 tgatttgtgc tgcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca
     7861 gcagcagggt aaaaaatact ggggctaaac agagcatcac cactaaccag ctccatactg
     7921 gtaaggcaaa tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat
     7981 gacgatgacc acttaagaaa atcaacaaac cgataaacat gccgcctccc gcaagggcaa
     8041 agccaacatg ccatccatac cactgagcag ccaggccgca ggcgatgggc gcggcgatag
     8101 aaccgatatt gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct
     8161 cgtcgtagag ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa
     8221 taatgattgc cagcgccaga tacaggctaa aggttgaatt tgtatcaatg cccagcacca
     8281 catggccaag ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc
     8341 ggtcggcaag ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga
     8401 acaggctgat ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta
     8461 aggcacgcat gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata
     8521 tcgcgcgcgg ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg
     8581 caagccactt cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg
     8641 gtatagcggc catcagcctg acggtatgca ccgtgaatat cggtttcaaa gcccggatag
     8701 tgagcgccga tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg
     8761 atcatttcac ccggcattac cagaggaact cccggcggat acggaaggat catattggcg
     8821 ttaatacgac ctaccatttc gtcgaggtaa acttcttcgg tcataccgtg cagctctttc
     8881 tggaacgcag catacggagt cattaccata gtcggcagaa cttcaaatgc gcgatacatc
     8941 agatccggca gattgtggtg aacaatcagt ttgtggatat tctgagccag ttcctgaata
     9001 cgcatgtttt catagaattc aggatcttca cgatacagag acggcagcat gtttttcaca
     9061 cgcaggttca gatcgaacgc acgtttgaag tcagtcagag cacgcagcag gctcagtgct
     9121 ttggtcttat cgataccgat gctgaacagg aacagcaggt tatacggacc ggttttctca
     9181 acaacgatgc catgttcgtc gaggtatttc gccacgatgc tggccggaat accaaagtcg
     9241 ctcatggtgc cgtctttttc catccccgga gtcagcaggg tgactttgat cgggtcaaga
     9301 tacatgtgct cgttatcgat gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc
     9361 cagcattcag tcgtatcgat atgatccggc tgccatacat caaagaacca gccatcagat
     9421 tccgttctca gacgtttgat ctctttacgg aatttgatcg cacgttcaat ggaaccattg
     9481 atcagacgtt tacctgcatt gcctttcatc atcgctgcag cggtttcagt ggacgccacg
     9541 ataccgtagt gcggagaggt ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg
     9601 tttacgtcac ctttaacgtg gatcatggaa gcctgagaga acgccgccag cagtttgtga
     9661 gtagactggg tttcgtaaat cactttccct tctacacggc caccgctcat accgcattta
     9721 ccttcgtaaa tcggtgagaa gttggtgtaa ggcacccacg cggagtcaaa gtggatggat
     9781 ttcacatcca gtgttttctt gatgaagtcg gtgttgtaca gcagaccatc ataggtagag
     9841 ttggtaatta cagcatgtac cggccaggtt gcgtttggtg tttctttcac gcgcttagca
     9901 atggtagcgt gctggaattc actctgtggg ataccaccaa gaataccgta agcgttacgg
     9961 gtcgggcgga aatagattgg cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg
    10021 tggcagttac ggtcaatcag aatggtgctg cctgccggag cagagtacat accaacaatt
    10081 ttgttcgcag tggaagtacc gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga
    10141 gcgatatact gttctgcttc tttgtgtgga ccactgtgat ccagcagaga acccagttca
    10201 gatactgaaa tggaaatatc agatttcatg gtgttcggac caaagaaatc atagaacagg
    10261 ctacctaccg ggcttttctg gaatgcagta ccgcccatgt gaccaggagt acagaaagta
    10321 tatttacctt cacgaacata tttaaacagt gctttggtca gcggaggcag aatagtgttg
    10381 atatattcgt cagtggtctg cttgatcttg ttagcaatat catcagcagc acccagcgca
    10441 tattcaaaga agctaatctg taaacgcagg tcattcaggc ttacatcgag agtggaatac
    10501 gtattagcga acgcgtacaa cggcaggttc tcgttcattt tgctaatttc ttcgcacagc
    10561 tcgagattat atttatccca gtcaaaaata acaccgcaca gacgcgcatt gttttcgatc
    10621 agttttaata agtcgtcacg gtcgttcggg taaacaatct ggaagttcag acgttcaagc
    10681 gcgcgatgaa gttcacggat gggttcttct ttaaaataaa cccccatgtg attcaatatt
    10741 gcaataacgt tcatagtcat atctccaggt aaaaaaggcc cctcccaaca catgggacaa
    10801 aatgaaagga ggagcctcgg aaaatacttt taattaatgt gcgttagacg ctgtgtggtt
    10861 atccattgag tggctctggc gctcgtgcat tttgcgagcg tagaacatca ggataatcag
    10921 gctgacgatg aaggtacctg ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca
    10981 gaacacgcaa cccagtacag agcagatcag gctgacaaag ttgcggatgt taacgccttc
    11041 aaaacgaatc aggtcaacgc aagagtagaa gtacggcagc atagtcagca gtactgcgat
    11101 accggtcagt tcaccgaaca ggtcagaggc tttaccaccg gcagagttca tcagagtgat
    11161 gaggatcatc agggcagtca ttttcactgc agccagcagc agaccttttt tcggaatacc
    11221 gttgctgtcg acttcaccat aaactttcgg gaagttaccg tcgttagcgg cacgtacacc
    11281 tgcctggcct accaacatca tccaggagcc cagagaagtc agacacgcaa aggcggtgaa
    11341 tgcagaaacc agtggcgcag cccagttacc gaggatagtt gaagcactga ttgcaaacgg
    11401 agcaccggaa gccgccatta cagaagacgg atacataccg gaaagcacct gagtcgcagc
    11461 gatgtaaaca atacctgcta aaccagtacc cagcatggtt gccagcggaa cggtacgttt
    11521 cgggttttta accataccag tacttactgc tgcggattca acacccacga aggcccacag
    11581 gcagagcaga atacttttaa tgatcgcatg accatcagtg gtatccgcag tattccagtt
    11641 agctgcataa gttgctgcat caaaccaatg ccagccaaca atagcagtca tcaccacagg
    11701 aataagaacc agcaccagac caatagtggt taaacggctt acccaggtac cgccgagcat
    11761 atttacaaag gtaaataccc agacgatagc aatacaggcg atacccgccg gaacaggatc
    11821 atttaatact gggaagaagg tggaaagata agatacagcg gtaataccaa ttgccaggtt
    11881 accaatccag ttagcatggt aataaagaac acctgtctga aaaccaaatg caggggaaat
    11941 ttctccggca tacgcaattg ggccaccttg ttgcgggttt tttgttgcca gtcgggcata
    12001 tacatatgcc agcgacattg caccaataat agagataatc caaccccaga tagcaatacc
    12061 accgatactt gctaggttcg caggtaataa tgcaataccg ctccccatca tattaccggc
    12121 aacaacaccg gtacaggcaa atagcccgat cttcttggca gaactcatgc tcttctccta
    12181 atttcatttt tgaatttgga gtccgggtca tgatgtataa ctatttcctg accagaccaa
    12241 actggcgcta agattactca cgaaaaaagg attaatccta aagattaggt gaaataacac
    12301 aaaagtttct gtaagtgaga acttgaggtt ttttattaac acatcaggat cgcaagttga
    12361 tatcatgaaa agataaacat tcaatgttta caatgcattg cgtgacattc tctggttaaa
    12421 tttatgtaat aaaaattatg cggcaaataa attgccgcaa catattatac caacgggaac
    12481 atacaaaaac tcaacaacaa atattcccga gcatgaatca acccgggagt tacttattct
    12541 gaagcgagaa atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca
    12601 ttttcaatcc agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca
    12661 tcagctgctt cccggttcat ccccttcatt tcataaacct tgccaagcaa cacataattt
    12721 agccaggaca tttcaagatc aatgccggta tttatcgcct gataagactc atctgtttta
    12781 ccttttacca gggcactgac cgcttttatt tgatatataa tggacaggtt gttcaattcc
    12841 ggcagtgtaa caatgttatc tatttctgtg ttcagtgctg ctaattgttt ttcgtctaaa
    12901 ggatgttgag aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat
    12961 tctggggatg attgaacaat ctcacctaat aattcactgg cacgatccaa tgatttatca
    13021 tcaccatgca gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatgc
    13081 ggcaaaattt gctggagcat ctcctgcatt cgttgtggcc acggttggtt taacgctttt
    13141 gataaactct ggagcaaatc attttgaatc gccagctgat taccgttagt gatgacataa
    13201 cgtttatcca gcatggttga accatctgca ttgtctacca attttaccga cataaagcat
    13261 tgttgagcac ggtattggcg ctgattaaca aacgcaatag ataatgtttt accggaactg
    13321 ctcggttcat caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta
    13381 agtgatgtcg ccaccaaatc acccacgcct atcgcgtaag agagctgata cggagaactc
    13441 cagctgttac aactcttatt aaccatatta atgtcaatat cgcgtggatt gagcaaaatg
    13501 cgcgatttac tcataggaag acgtgtttca agacttgaaa acgctaccag tgcgacacag
    13561 atacctaacg acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta
    13621 actggggatt gttcaggtgg cgttgtggtg ttttgaatgt taagactgtg ggagggagaa
    13681 tctgtggcag gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc
    13741 tcttcttcgc tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta
    13801 gcgatataga caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata
    13861 ctctgcgtca caacgtgatt ggtgacaata cttctcttcc agacattatc gataagttca
    13921 tctctgctaa gtacttcgcc actgtgttga gcaaagaaaa ccagaagatc gattaatctc
    13981 ggctcaaggg taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc
    14041 cattcgccaa cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga
    14101 tgcaatgata attgccataa cctatttttt ccatctatag atgggtttat ttacatatta
    14161 ttggtgaatg caagacgtta tttttactcg ccataaactt cttgattaca tagtattacg
    14221 aaaggatttt actgagaacc agaagtaatt ttccttacca tcaaaattca tcatctttgc
    14281 caaagaaaaa tgttcagaaa ataatccatg aaaaattgtc cggagcgctt actattttaa
    14341 tggattgtta gtctttgcat gagcaaacgg actgatacat ttctctttgt tctcattcag
    14401 aaaatctcat cagttggcgt tctgcccgat gttgtgcttt atggatgcat tacgctcctg
    14461 atgacgtcat ttgacgttca acagcatcac ggggccgcac gacatttcac gtcagtcagc
    14521 gctatagctc agaaacaaat tttcccgaat tgggatatgc ccgcaaattg ctggtgatgt
    14581 ggggagaatc tggttgagtt cggtagaatt gatttggcgg caggacgctt aaatcgtggc
    14641 gtcctgaaac gaaaaatgga cctccgtgga ggtccgttta tatgaatttg gtgcccggac
    14701 tcggaatgaa actggttgta taacaagttg ttttatattg attgttttgt gtttgttatt
    14761 tttgaggcca ccaatgaggc ccccatgtgc aattcgaatt cttttgacgc gataatccct
    14821 cttttttgtg cttggctgta attattttgt ctggttcgat gtgctgatgc gcggaatcgt
    14881 acttaacgcg tttgttagtt ggtcagaaca ctcaacgctg tttgtctgaa acaggagtag
    14941 gcttcttccc agtcaatctg tgtattacca aataccatcg gtttaacaaa tcgttgaaaa
    15001 cgcaatttgt gtacaggatc atcccggagt gcatccagcc ctttcttaag ttcatcaact
    15061 ggtgagttac gaaattgtgg gtgctggtta ccatagcgct caatgtcctg ttgaatacat
    15121 atctgaacaa aatttttcag tactggaaca ttcacacctt tttccctgac aatgcaataa
    15181 ttgtcgtaaa tgtggcgtac cagggactca tcatcctgtt gttcgggatt gcgaagaagt
    15241 atggctgttc ttcgcatcat ggatatgagc ttttcagcct gagtggataa aatggtggca
    15301 caggggaatg ctgttacagg tgtgccatta cccgttaact ccgtgacgag tgagtaaata
    15361 tctctgcttt ccgggtgttc cagcagagtg gtttccatca actccagctt aataaatgga
    15421 cgaagacagg gggcctgggc aaatgtctgg ggataacgta cggggatctc gttataacga
    15481 tactcatcac gtgttatacg ggcgttttca ttatcaaggc tgaaaatgtc agaattatga
    15541 agaacatccg tgattatttg cacgatttcc ttgcgaattt ttcttttcct gtttctgcca
    15601 ttttgcataa agttctctgt tggcacgagc tttatatcaa tatcttctga cattctgttc
    15661 aatgagatac cagcttttga caatgcggta cctcctgcaa acacgagagt atgtgtttct
    15721 ggttttattt cttttagtaa ctttaaaaga ttaaccacaa aataatcttt ttcgacgatg
    15781 gcgacagatt caatatccag tgcgttggca acgtcaggaa ataatgatgt taattcaggc
    15841 attgatttta cttttcccta caactaacac tcttttaaag cgaggaggtg ttttgaactc
    15901 cggagatacc ggaatctgtg tggactgacc tttattgtag cattctgttg cgccgatcaa
    15961 gctgtatctg atattcaaac gatccagggc ttcggtgatt acagcggcag agccaccagg
    16021 tgctgcaggc atgatttttc ctgtaataac gttgcgacgg gctttggtgt aaacaccata
    16081 gcccactttc atcaacttac cttcgtttac cagcgtacga agagctctgc caacctgatc
    16141 atagccagcg atatccctga agtcatcccg ggtgaacaca taacgctttg aacggttcag
    16201 tcgggactga atacggtctt ttgtagtcat gcttcctccg aaaaatatta agaatgatag
    16261 cagtaatacg gcatgtttaa tagcggggca gggtcacatt acctttcggt caatacattt
    16321 ggatagtatt tcgcaataat gtcattcagt ctgtcaacca gctctggttt cctgaacgtg
    16381 atgtgcgcgg agcccttctt aaagtatctg atgctgaaca tctcatcttc atagtcctct
    16441 ttaccctgaa cagactggat gtgatcatcc agacgaatag tgatgttttc ccggttgtcg
    16501 gggatcggtt tgccgctgaa caggtgtagc attctttcca gatcggcaag tcgatcagcc
    16561 tgttgcccgg tgataagatg aaacccccac cggtcccacc tcaccagatt gttgacgata
    16621 attttactgc caaatttgca gggtgaattg gttttgtaat tccagctcag tcctctgaaa
    16681 acgttgagca cccctcgctc aaacacttca tctttgttct ggtgcagttg ttcaaacgtg
    16741 ctcaggatat ttgcttcgct gatttccgga aaattatcgt actccagtga ccggtaccat
    16801 gtatcacggg cttgtgcatc cattaatgac agcatccctg aacgttgcat caggtcacgc
    16861 cagagttcgc gatcgagatt ccgggtgatg gctttcattg cggtttccgg tttctccatc
    16921 agccagcaac cgcagcgaaa atcctgtttc atggcccagt cccgggcggt tttaccgcca
    16981 atactttctg tcattgctga tatttcagcc agttgccgga taagtgtgtc gatttgcagc
    17041 agtgtactct ttcgtacagc cagaatgtgg cggatactgg ttgagcaaat aacgtccgta
    17101 tgcccggtga ccacttccgg acgggaggct gtgatatctg acatcatcgt ttttcctttt
    17161 ggtttataaa cgaaaacgcc agctgtattc aggctggcgt caggggaatg aagtatggtg
    17221 agtaagactc accggttctg ttgcagaagc tgtagttggc gcagggtacg gatatcttcc
    17281 ctggcctgtt caatgcaggg caacagtgct gctgaatccg ttctatcctc atcgttgtcg
    17341 aagataatcc cggattcaca gtcaatattg tcctgcagcc acgcaatcag aatatccagc
    17401 gccgtttctg tcgttaatga tttcatgttg tgaatttccc tgattaccag tcgaaagtgg
    17461 gcagacccgg cagacattca gcgcaggcat ccagatgaat gagactgacg ccataatggc
    17521 gaatgagcgt gacgatcaga cgacgaaacg tttttgacag gcccaggcgt ttaagtcgca
    17581 gaaccgggta cgaccacgca tcggtacgta acagatatcc gctaccggta aaatgaatcc
    17641 attctgattc gccgaagtca ttcgtctgat gtgacagcga gtacagccag gcgttgtcct
    17701 ttgccgtgat atgcgcggta ctgcagcgta tgccggtaag ggccgcagat ggtggcgtgc
    17761 gctctgctga tgtccggtca gattcatcaa caacacgaag ggagtaaccg ttatcgcaga
    17821 ccacgttaat cagttcagcg agagtaacac catcgacgtc aacgacaatg cgccccatgt
    17881 tcagtgcctg cacgttaatg ctgtcggctt ccagcgtcag ggccagtttc attgtttcgc
    17941 ccccggatgt ttacccaggg taatgttatt taccgttcgg taattgtcgc gggtcatcag
    18001 gccagtcgcc cggcgagccc ggaggatatc gatgctgttt attaactgag agcgggtaca
    18061 ggcactgaat tccggctggt cggtacgcac cagtgcgtat ttttcaacaa gaaagttcac
    18121 tgcatcgcac agtgaaatgc cagcttcaat atgctgctca atcacacgtt catcggcgaa
    18181 cggtgtgtca ttcagtgtaa ggccgtaatg ctggcccagt agtcgggaga gcagtatctg
    18241 ccagatttca acaggagacg ggcgagaact ggccgcctgc ccgggtaata caggtaatgt
    18301 gttcatactg aagattttcc tgatatgcag agatgaaaaa tgggaaagtg gcgtgatgaa
    18361 agcacctggc cggagcagag agttatcttg gtggtttacg ttttcgtttc ggacgtcgga
    18421 taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgcg tgtgccacga
    18481 atgcgtccag tagctctgat tatggatgca atgctgtaca aaagatgagg gaaggtccct
    18541 cggaggcggt atccagatgc agcctccaaa ccaccctgag ttgctgccat taagagtggt
    18601 ggtgatgagc gtcagggaaa aggcgcaaat atgtgtcagg tatgtgttac ggagatgaac
    18661 gtgagtgaac tgttgatgac atgtcgaaac gacttagatg atgtcgaaag cgcgctatgt
    18721 gaggagcgcg tgataaatcc agcggaaatc tggctactgg ctgggtggca tccggcatat
    18781 agacagcatg aacgtagcac aggcttttgt atggaacgtg ggaacctgtc gtcccgatgt
    18841 caagggagcg gtgtgaagca gaagaactgt gagcgcctga gtaccaatgc ggggcacagg
    18901 ggcagatggt tccgtagtag tgttgaagta tctgtaatgg gtgctgagcg aagggaacca
    18961 gttgtcgggg aggatttatg agccaaccaa aacagcggga agagtccatg aatcatcatc
    19021 caaaaccatt tatgatttcc aagatcgcgg tctggaaagc atatcaacga attagagcca
    19081 atcgtggctc gccgggtgtt gacggtcaaa cgatagagac gtttgaaggg aacctgtcgg
    19141 gaaatctcta caaactctgg aatcgaatgg cttcggggag ttatatgccg ccaccggtaa
    19201 ggcgcgttga gataccaaaa gcaacaggag ggacgcgacc gttggggatt ccaacggttg
    19261 cagaccggat tgctcagatg gtggtcaagg atgtgctgga gccaatactg gagccgcatt
    19321 ttcataacga ctcctatggc taccgaccgc ataaatccgc acatgatgct ctcagagctg
    19381 cacggcatag atgctggcga tcaaactggg tgctggacgt ggatatcaag gggttcttcg
    19441 ataacattga tcatgacctt ttgatgaagg cggtttgtaa acatacccgg tgcaagtgga
    19501 ctgaacttta catcagacgg tggttgactg ctccggtgca aatgccggat gggacactac
    19561 atgcaaggga caggggcacc ccacagggag gcgttatcag ccctctgttg gctaacctat
    19621 tccttcatta tgctttcgat ctgtggatga cccgtcagta tccagatcgt ccttttgaaa
    19681 ggtatgctga tgatatagtg attcactgca atagccaggc tcaggcaata gtgctgcgaa
    19741 acaaactgga gcatcgactt gcggaatgca agctggaatt aagtcagagc aaaacgaaga
    19801 tcgtttattg caaggatggt aaacgcagag aaaattatcc tgatattagt tttgacttcc
    19861 tgggatatac ctttcgcccc agaaaagcca gggcaaaaga tggagtaaaa tttcttagct
    19921 ttatgcctgc aataagcggt aaagcatcca aaatgatccg tcagacaata cgaggatgga
    19981 aatggaagca acgaaccacg cttacagttg aagaagttgc gcgccgtata aatcccattc
    20041 ttcggggatg gataacgtat tatgaatgtt tctaccgttc tgccctgtat ccagtattgg
    20101 cgcatgtgga tctgcatttt gcaaagtggc tcgccgataa gtacaaacgg ctcagaggta
    20161 gcagaaagcg tgcattcctg ctgttaaaac atatacgccg gaaacgtccg ggtctgtttg
    20221 tacactggtg tatggcatac aagagttgag tggttcgaca acaggagccg tatgacgcga
    20281 gagtgtcacg tacggttctg tgggagcgta ggggggaggt tcccctgcgc cacccgatag
    20341 gtcagccctt ttgcatacag cgtgacggta tgctgatgtc ggggattcag ttcaccgctg
    20401 gtgagcatta attccagttg tttcatcagc agcggaaagg cctggtccgg gtggtacgca
    20461 tccgcatctc tgaaccggcc tctgataccg gcacggtcgg caaggtaatg caaccggtta
    20521 ccctcctgca ccagacgtgc cccgaaacag ggcatcacgg tgcagggtag cccccaccag
    20581 gggcggttgt tgttgtcggg atgcgttgtc ccgggcagtg tgtctgacac gataaagtcc
    20641 tcacagaaat cagttaagaa tggtccggtg gcagcgaaaa ttcggctcat catttctcct
    20701 gactcagttc agagtgacgc tcatcagccg gacatacggg ccaaaactgt ccttacggcg
    20761 ttcggcaaac acagccagca cgccgggaat atcctgcact tcacgaccgg tatacgcttc
    20821 agcactgccg tgccagcggt acttaccggt acagaacgga aaaagacggg atgtcggatg
    20881 ttgttggtga atacgcatgg cttcaccacg ggtgataatt ttcataatgg gatacctctg
    20941 aagacagaag ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca
    21001 tgttcggcaa aggagaaaac ctggttgcca ccgactatca gatggtccgg cacccggata
    21061 tccaccaggc ccagtgcctg taccagacgt tcggtgataa gccggtcagc cttactgggg
    21121 gtgatttcac cggacgggtg attgtgtgcc agcaccacgg cagcggcatt gtggtacagg
    21181 gcacgtttaa tcacttcccg gggatggact tccgtgcggt tgatggtgcc ggtgaagagg
    21241 gtttcaccgg caatcagctg attctggtta ttcagataca gcacccggaa ttcttcacgc
    21301 tccagtcccg ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc
    21361 acgccgggtt catgaagatg gcggtccagg gttttcaggg cccgcagaat gagactgcgc
    21421 tcgccgggcg tcatttctcc gggcagaaag gaaatctgtt gcattgttcc tgcccccttt
    21481 cagtcaataa tgcgcataat ggcgtcgtat tccggatgtt gcagggcgta atcccgcaga
    21541 cggtaataat gaaccgtcat tgcataacac tccgtacggc aggcgtgatg actgtacgtc
    21601 atcagacagg cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg
    21661 gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg gttcgggggc cataaatgcg
    21721 ccaccgttgt tcagggtgta cagattccag ataccaccgc agtagtcttc acacagacgg
    21781 tccatccagc cgaagatacg gggctccagg gtcacccact gtggaatgag gccaaagtgc
    21841 tgtggccaga aactgatgcg ctgttcatca gggacgaggc tggcaaccaa ctgaggcgga
    21901 ttattctctg gtgttgtagc tgaataaatt gtgggggtat tctgagaaac gcttttcatg
    21961 agataactcc ttaaataaca aataaatgtg tgtttttcag gaacgaaagc tgaatgattc
    22021 cctcactcag ggaaacggca tcagcgcagg ctctccagca tcgtttctgc catcacccac
    22081 aatgcgcggt tgagcttaat atcggtgtcg atgctgtgaa tggtccgggt atggatacgt
    22141 tttccttttg cactgcgacc ggaaatcccg cctttcagca tattctcctg gatggtctga
    22201 taagcactcc acaggtcctt accgtaatcc tcccggcgtc gtggtgtcag aatgccggcg
    22261 gtggtgacgg gctgatgttc gtcaccataa cggtaagtca gcgcagcctg tgccagcgcc
    22321 tggcgtgccg gtggcggcag gaccagcgac tgcatggcat cacgcttctc ctcaatccgg
    22381 tcaaaaacgc ccaccacctc gtaagcacct tcaatcaccc tgtccaccac gtttccccgg
    22441 tggggcaccc ggacttctcc cagcgactga ccgcagacca agccattggt gcagatggca
    22501 cgaaaatatc cgggtaacat ctggtagctg gatgaaccgt catgggagtt gagcagaata
    22561 atttcaggca catgctgacc ggttatctgt ccggcccgcc gcagacgcag catatgtttg
    22621 gtatattccc ggcggctctg gtcgcgcaca cgggtctggc aggcgaagaa cggctgaaag
    22681 ccttcccgct gcagattttc caggacggtg atggtgggaa tgtacgcata ccgttcactg
    22741 cgggaggtgt gccggtcttc tccaaaaata ctcgggacgt ggtacatcag ttcttcgcgt
    22801 gtcagtgggc gctcccgacg gatctgattt acatgaccaa aacgactggc taattgcata
    22861 ctttgctcct tatgaatgaa ttagcggtat aaacgaaaag gccgcgtctc ccggagaaga
    22921 cgcggcctga tggatgaaat gaagacgttt attgcctgag aattccctca ctggcgagat
    22981 acgaatttag ctgttttccg gcatcaggtt caaaattcca gcaccgccag cgtagctgcc
    23041 cttcggcatt gtggataacc cgccggaaat gtgttccctg tcatcttcga taaacacatt
    23101 gcggtaagct gttgtgacgg caggagcctg tttgcgtgag aaagggcctt cgggcaataa
    23161 aacagggcga cagtccgaca tcccggttat tttccgataa attcaacact ggcgtcggtg
    23221 gcattgccgt cagcgccaac agtgacggtc actttgtcgt atgtacggta gccaggattg
    23281 aacacctcaa actcaacttt attgcccttt atttcataag agtcaaaatc agcggtttct
    23341 gttgtttcct cgtcatattc ccgggagtat gcctgccggg cggcctgcag tacggagtct
    23401 ttatttatga cggacgtctg tttcggggaa aaaccggcaa gcgacgtgac aaatttcggg
    23461 cagaccttca gcatatcacc gcgggtgaat tccattcgta caccaaatgc ctgtaaaacg
    23521 gacacatcac gctctgctat ggagtgagct gcttttttga ccatttccgg cgcctgtccg
    23581 gcttccacct caacagccct gaatcccgtc tgctgattac acatttgtga tgtggggatc
    23641 aggttaccgt taatgctgat acggctgccc aggagcgtct gtacgccatt cgggcacccc
    23701 tgcatcaggc cggtgatacc aatcgtgcca tttttcagca tcaccagcgc ctgaccatcc
    23761 tccgtcatgg tctgacacca gccatttttg acagcccagt tgccggcgga ggcggagaat
    23821 gatgccacgg tgaacagcag gggaaaaaga gctctggtat tcatgactga cctctgaaat
    23881 gaacgtacgg tgttattgat aatgatggaa ggtatgcagt gactgttcag ggaatgacgt
    23941 agccgtatat ttagttttct gttcaacata ttgtctccat acctgtcggg acggctttgg
    24001 caggccggcg atcacaggtt aaaggtgttg ataagtttat ggatgaaaga gaaatgtggc
    24061 agggcggaga taaaacgctc ttcagcgacc agactgtgga tgtggcctct ccgttcaaac
    24121 cgttctgatg tcaggctgac tgatttgata tctgccagag gaaagacctg cagttttccc
    24181 ttctgtaacg cgatcagata ccagctaccg ctgtaataaa tgagccggca gggctccagt
    24241 gacggatacc atcgctcagt catcagagag atgtggatac actgtcttat tgccaggatg
    24301 agttgcgaga aacactcggg gaacgttgca gtgatatccg gcgccggtag ccagataagg
    24361 cagggagagg gctcctggtt gtcggtaaga caggttatca gtcgtccgtt ccggagcgga
    24421 agtatccgtg cgatcccggt attctgtata aaagaaagca tttcagggat cgcacctggg
    24481 ctgctctgtc gtctgaggct gtacctgcca tttctgtact ctaaatccag gtgaaccaga
    24541 cgctgatgaa aatcgcgctg taaagtacgt tctgtaacgc caaattcatc tgacagtgtt
    24601 tttagtgaca gagattctcc ggccatcagt cggctgataa tgagtgataa gcgaaccgcc
    24661 aggcgatcat gccgggaacg attatcagac atagatgtgc actccgtaag aaaacacctt
    24721 ttatttcatg atgttattat gcatcatgat gttgaaagga tgtgtcgcat caggataatc
    24781 aggaataaaa tgcagcagaa aaaatattct tttaattatc tgatttttaa gcatgatttc
    24841 ataaaaagaa gaatgctcgt taatccagcg
//
LOCUS       DAJOTB010000064        24770 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJOTB010000064 DAJOTB010000000
VERSION     DAJOTB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.064"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            15..848
                     /gene="xapA"
                     /locus_tag="OHN28_002519"
     CDS             15..848
                     /gene="xapA"
                     /locus_tag="OHN28_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HCQ2297905.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            909..2165
                     /gene="xapB"
                     /locus_tag="OHN28_002520"
     CDS             909..2165
                     /gene="xapB"
                     /locus_tag="OHN28_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HCQ2297906.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2218..2376
                     /locus_tag="OHN28_002521"
     CDS             2218..2376
                     /locus_tag="OHN28_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HCQ2297907.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            2417..3301
                     /gene="xapR"
                     /locus_tag="OHN28_002522"
     CDS             2417..3301
                     /gene="xapR"
                     /locus_tag="OHN28_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HCQ2297908.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(3412..3487)
                     /locus_tag="OHN28_002523"
     tRNA            complement(3412..3487)
                     /locus_tag="OHN28_002523"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3452..3454),aa:Lys,seq:ttt)
     gene            complement(3492..3567)
                     /locus_tag="OHN28_002524"
     tRNA            complement(3492..3567)
                     /locus_tag="OHN28_002524"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3532..3534),aa:Val,seq:tac)
     gene            complement(3612..3687)
                     /locus_tag="OHN28_002525"
     tRNA            complement(3612..3687)
                     /locus_tag="OHN28_002525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3652..3654),aa:Val,seq:tac)
     gene            3946..5361
                     /gene="gltX"
                     /locus_tag="OHN28_002526"
     CDS             3946..5361
                     /gene="gltX"
                     /locus_tag="OHN28_002526"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCQ2297909.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(5412..5804)
                     /locus_tag="OHN28_002527"
     CDS             complement(5412..5804)
                     /locus_tag="OHN28_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2297910.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(5806..6165)
                     /locus_tag="OHN28_002528"
     CDS             complement(5806..6165)
                     /locus_tag="OHN28_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2297911.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            6386..6461
                     /locus_tag="OHN28_002529"
     tRNA            6386..6461
                     /locus_tag="OHN28_002529"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6419..6421,aa:Ala,seq:ggc)
     gene            6501..6576
                     /locus_tag="OHN28_002530"
     tRNA            6501..6576
                     /locus_tag="OHN28_002530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6534..6536,aa:Ala,seq:ggc)
     gene            6783..8972
                     /locus_tag="OHN28_002531"
     CDS             6783..8972
                     /locus_tag="OHN28_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCQ2297912.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(9022..10224)
                     /gene="nupC"
                     /locus_tag="OHN28_002532"
     CDS             complement(9022..10224)
                     /gene="nupC"
                     /locus_tag="OHN28_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCQ2297913.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            10560..11798
                     /locus_tag="OHN28_002533"
     CDS             10560..11798
                     /locus_tag="OHN28_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCQ2297914.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(11939..12265)
                     /locus_tag="OHN28_002534"
     CDS             complement(11939..12265)
                     /locus_tag="OHN28_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2297915.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12380..13636)
                     /locus_tag="OHN28_002535"
     CDS             complement(12380..13636)
                     /locus_tag="OHN28_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCQ2297916.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            13840..14805
                     /gene="glk"
                     /locus_tag="OHN28_002536"
     CDS             13840..14805
                     /gene="glk"
                     /locus_tag="OHN28_002536"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCQ2297917.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15024..15350
                     /locus_tag="OHN28_002537"
     CDS             15024..15350
                     /locus_tag="OHN28_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCQ2297918.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            15372..16619
                     /locus_tag="OHN28_002538"
     CDS             15372..16619
                     /locus_tag="OHN28_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCQ2297919.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            16634..17719
                     /gene="ypdF"
                     /locus_tag="OHN28_002539"
     CDS             16634..17719
                     /gene="ypdF"
                     /locus_tag="OHN28_002539"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2297920.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            17719..18756
                     /gene="ypdE"
                     /locus_tag="OHN28_002540"
     CDS             17719..18756
                     /gene="ypdE"
                     /locus_tag="OHN28_002540"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2297921.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            18781..21276
                     /gene="ptsP"
                     /locus_tag="OHN28_002541"
     CDS             18781..21276
                     /gene="ptsP"
                     /locus_tag="OHN28_002541"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2297922.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(21279..22136)
                     /locus_tag="OHN28_002542"
     CDS             complement(21279..22136)
                     /locus_tag="OHN28_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2297923.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22149..22883)
                     /gene="ypdB"
                     /locus_tag="OHN28_002543"
     CDS             complement(22149..22883)
                     /gene="ypdB"
                     /locus_tag="OHN28_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCQ2297924.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(22898..24595)
                     /gene="ypdA"
                     /locus_tag="OHN28_002544"
     CDS             complement(22898..24595)
                     /gene="ypdA"
                     /locus_tag="OHN28_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCQ2297925.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5839 a         6027 c         6613 g         6291 t
ORIGIN      
        1 aaaaaaggat atatatgtat caggctcagt tttctcataa cccactgtat tgcgtagata
       61 ttatcaagac ttataaacct gatttcacgc cacgagtggc ctttatttta ggttccgggc
      121 tgggcgcgct ggccgatcag attgagaacg cggtcgcaat ttcctacgaa aagctgcctg
      181 ggttcccggt aagtaccgta cacggtcatg cgggtgagct ggtgctgggg tatctccagg
      241 gggtgccagt ggtgtgcatg aaaggtcgcg gacatttcta cgaaggtcgt gggatgacca
      301 tcatgacgga tgcaatccgt acctttaagt tgctgggctg cgagttgctg ttctgcacca
      361 atgcggctgg ctcactgcgc cctgaagtgg gggccggcag tctggtcgca ttgaaagatc
      421 acatcaacac catgccggga acgccgatgg tgggtcttaa tgatgaacgt tttggtgagc
      481 gcttcttctc gctggcgaat gcctacgatg cggaataccg cgcactgtta caaaaagtgg
      541 cgaaagaaga gggggtccct ctgacggagg gcgtgttcgt ctcgtatccg gggccgaatt
      601 tcgagactgc ggcggaaatt cgcatgatgc aaattattgg tggggatgtt gttggtatgt
      661 ctgtggtgcc tgaggttatt tcagctcgcc attgcgaact taaagtcgtt gcggtctctg
      721 cgattaccaa catggcggaa ggtctgagtg acgtgaagct ttctcatgcc caaacgctgg
      781 cagcagcgga actctcaaag caaaacttta ttaatcttat ttgcggcttt ctgcgcaaaa
      841 ttgcctgagt aaaacaacaa taaacggcct tactgagtga ggccatatga tgcgacaagg
      901 aaacgattat gagcatcgct ttgcgcttaa aggtaatgtc ctttttgcaa tattttatct
      961 gggggagctg gctggttacc ctcggttctt acatgattaa tacccttcat ttcaccggcg
     1021 ctaatgttgg catggtttac agttccaaag ggatcgccgc gattattatg cctggtataa
     1081 tggggatcat cgcagacaaa tggctgcgtg cagaacgtgc atacatgctg tgtcacctgg
     1141 tgtgtgcggg cgtacttttt tatgcggcat ccgtaactga tccggatatg atgttttggg
     1201 tgatgttagt caatgcgatg gcgtttatgc cgactattgc gttatcgaac agcgtctctt
     1261 attcctgtct tgcccaggca gggcttgacc cggtgaccgc tttcccgccc attcgcgttt
     1321 ttggtacggt ggggttcatt gtcgccatgt gggcagtaag cttgctgcat ctggaattga
     1381 gtagtctgca actgtatatc gcgtccggtg cgtcattgct gctgtcggct tatgcgctga
     1441 ctttgccgaa gattccggtt gcggagaaaa aagcgactac atcgcttgcc agcaagctgg
     1501 gtctggatgc cttcgtgctg tttaaaaatc cacgcatggc tatctttttc ctcttcgcca
     1561 tgatgctggg tgcggtgctg caaattacca acgtttttgg caacccgttc ctgcatgatt
     1621 tcgcccgtaa tcctgagttt gctgatagct tcgtggtgaa atacccgtcc attttactgt
     1681 cagtttcaca gatggctgaa gtcggtttca ttctgacgat accgttcttt ttgaagcgtt
     1741 tcggtattaa aaccgtcatg ctgatgagta tggtggcgtg gacgctgcgc tttggcttct
     1801 ttgcctatgg cgatccatca ccaaccgggt ttattttgct gttgctgtcg atgattgttt
     1861 acggttgtgc attcgatttc ttcaatattt ctggttcggt ctttgtcgaa caggaagttg
     1921 attccagcat tcgtgccagc gcccagggcc tttttatgac gatggtaaat ggcgtcggcg
     1981 catgggttgg ctcgattctg agcggtatgg cagtggatta cttctcggtg gatggcgtaa
     2041 aagattggca taccatttgg ctgatgtttg cagggtatgc tcttttcctc gcagtgatat
     2101 ttttctttgg gtttaaatat aaccatgacc ctgaaaagat aacgcatcgt tcagttacac
     2161 attaaaagga ttcgcagctc tgttccttgg ggctgctttt atgataaagg ttaattagtg
     2221 aaagatattt tattttcttc tggcgttggt tttggcattg gtgctttgtt tacaatcgtg
     2281 cgattaccta ttcctgttcc aaatgtattg ccgggtatat tatccatcgt gtttatgtat
     2341 gtcggatatc tggtggtgaa atactttatg ccatgataat ttaatacgat gtatttatta
     2401 tatggagcac ttaattatgg aacgcgtata tagaacagat cttaagttgc tccgttattt
     2461 tcttgccgta gcggaagagt tgcattttgg tcgcgcagca gcgcgtttaa atatgtctca
     2521 gcctccgctc agtattcata ttaaagagct ggaaaatcaa ctcggcacgc agctttttat
     2581 tcgccattcg cgcagcgtcg tactgacaca cgcgggcaaa atcttgatgg aagaatcgcg
     2641 tcgattgctg gtgaatgcaa ataatgtatt ggctcgggtt gaacaaatag gtcggggaga
     2701 agcagggcgg attgaactcg gggttgtggg aacggcaatg tggggacgga tgcgcccggt
     2761 tatgcggcga ttccttagag aaaatcctaa cgttgaagtt ctgtttcgcg aaaagatgcc
     2821 cgcgatgcaa atggccttgc tggaacgccg cgaacttgat gccgggatct ggcgaatggc
     2881 gacagaacca ccgactggtt ttaccagctt acggttgcat gaatcggcgt ttctggtggc
     2941 gatgcctgaa gagcatcatc tctcatcatt ttccaccgtc ccgctggaag cgctacgtga
     3001 cgagtatttc gttacaatgc cgcccgtgta cactgactgg gattttttgc agcgagtttg
     3061 ccagcaggtg ggattttcac cggttgttat tcgcgaagtt aatgaaccgc aaacggtact
     3121 cgccatggtc agtatgggta ttggtatcac attgatagcg gacagctacg cgcagatgaa
     3181 ctggcccggt gtcatattcc gtccgctcaa agaacgcatc ccggcagact tatatattgt
     3241 ttatgaaacg cagcaggtga cgcccgcgct ggttaaactg ctggcggcgt tggcgcagta
     3301 gatgagaggc acgggaactg ccggacatag ttggtctgat aagcgaagcg catcaggcaa
     3361 tttcgcaaat tgcagatagc aaaaaaacgc ctttagggcg cttttttaca ttggtgggtc
     3421 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
     3481 acgacccgaa gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag
     3541 gtgctctccc agctgagcta atcacccact tctcaatttc ttgctacacg gcggagacta
     3601 cataaagtag ttggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag
     3661 gtgctctccc agctgagcta atcacccccg ctgtgtggag tcgcattata gggagagttc
     3721 aaaatgagtc aacgcatttt ctaaagaaat tgttcgttcg tcgtaaattt aagcaagatg
     3781 atcgcaaaac agaccgtgtt gcgcaatttg tcaacgaaaa caataatgcg taaggttgca
     3841 acccgaacta cattgaggaa tcaggcggga gtgatagaat atcgcccact caatttttcc
     3901 aggatttgcc ggttgtcggc atctttctaa acgtaaggcc atttcatgaa aatcaaaact
     3961 cgcttcgcgc caagcccaac aggctatctg cacgttggcg gcgcgcgtac tgctctttac
     4021 tcctggcttt ttgcacgtaa ccacggcggt gagttcgtgc tgcgtattga agacaccgat
     4081 cttgagcgtt ccacgccgga agctatcgaa gccattatgg atggcatgaa ctggctgagc
     4141 ctggagtggg atgaaggtcc gtactaccag accaaacgtt ttgatcgcta caacgcggtg
     4201 atcgatcaga tgctggaaga gggcacagct tataaatgct attgctctaa agagcgcctg
     4261 gaagcgctgc gcgaagagca aatggcgaaa ggtgagaagc cgcgttatga cggtcgctgc
     4321 cgccacagtc atgagcatca cgctgatgat gagccgtgtg tcgtacgttt tgctaacccg
     4381 caggaaggtt ctgttgtttt tgacgatcag atccgtggtc cgatcgagtt cagcaaccag
     4441 gagctggatg atctgattat ccgccgtacc gatggttccc caacctataa cttctgtgtg
     4501 gttgtcgatg actgggatat ggaaatcacc cacgttatcc gtggcgaaga ccatatcaac
     4561 aacacaccgc gccagattaa cattcttaag gctctgaaag cgccggtacc ggtttatgcg
     4621 cacgtttcta tgatcaacgg cgatgacggt aaaaaactgt ccaaacgtca cggggcggtc
     4681 agcgtaatgc agtatcgtga tgatggttat ctgccagaag cactgctgaa ctatctggtg
     4741 cgtctgggct ggtcccacgg cgatcaggaa atcttcactc gtgaagagat gattaaatac
     4801 ttcactctga atgccgtcag caaatctgcc agtgcgttca acaccgacaa gctgctgtgg
     4861 ctgaaccatc actacattaa cgcgttgccg ccggagtatg ttgctactca cttacagtgg
     4921 cacattgagc aggaaaatat cgatacccgt aacggcccgc agctggctga tctggtgaaa
     4981 ctgttgggcg aacgctgcaa gacgctgaaa gagatggcac agagctgccg ttatttctac
     5041 gaagattttg ctgagttcga tgccgacgcc gcgaaaaaac atctgcgtcc ggtagcgcgc
     5101 cagccgctgg aagtggttcg tgacaaactg actgcgatta ctgactggac cgctgaaaac
     5161 gttcatcacg ctattcaggc gacggcggat gagctggaag tgggtatggg taaagttggt
     5221 atgccgctgc gtgtcgccgt aaccggtgcg gggcagtctc cggcgctgga tgttaccgtt
     5281 cacgcaatcg gtaagacccg cagtatcgag cgtatcaaca aagcgctggc ttttattgct
     5341 gaacgcgaaa atcagcagta attagcgcat aaaagataaa cggcaggaga gatacccctg
     5401 ccgtttttta tttatgctga ttcatctatg tcgagacgag caagaaaatt acgaattcct
     5461 tcgcgtgaca aaaattgcgc aactttttct tgctcactgt gagtcatgtt gtctattgcg
     5521 ctgatgattt gccgataagc aggagtgcga atacccggtg cgtattcggc aaggggttct
     5581 tcggcttcca gaagcatcgg tgtgttatgg aaggctggcg tgttctgaat aaactcgcaa
     5641 acttgcgtat cgaccagaat aagtctggca cgaccgcctt tcacgccggg aaatttttcc
     5701 gttttccagc ctttttctct gatccaacgg ttaacagttt gtttagccac accgaggcat
     5761 tcagccagtt cttcggtggt cattttattg cgtaatcttt tcatattatt tgctatcgcg
     5821 aatccctaag cgttgtaaca atccgataat cccttcccgc agaagcaagg atgtaaactg
     5881 tttttgttca actggcgtca tctctttcgc cagtgtcacc agcaacactt caagcggcgc
     5941 atcaccagaa agggctggcg cttctccctg accttctgga cgttcagcat tgcgaatata
     6001 ttcacgaacc tgttcattga cgtgaaccag tcgagccttg ccaccctgga cgcctggttt
     6061 tggtgacgtt gtccagcctt ccttgcgtac ccatttatta atggtctggc ggctatagcc
     6121 ggtcagtctg gcaagttcat ctggcgtcat cctctccttg aacataacaa ttccctgaat
     6181 gtatgtgggt ctattagtag ttcattttat aacaccactt tacaggaaac cgtgacatga
     6241 ataactactg aatgcgagcg gttttccaaa attgttcatt tgcctgattt ttcagcgatt
     6301 gaaactacac gtggcaattt gccgttgaca cattcgggcg gaattcatat gatgccgccc
     6361 gtcaactcga caagctttac gtgatggggc tatagctcag ctgggagagc gcttgcatgg
     6421 catgcaagag gtcagcggtt cgatcccgct tagctccacc aaattttgca cccagcaaat
     6481 ttggtacgta aacgactcgt ggggctatag ctcagctggg agagcgcttg catggcatgc
     6541 aagaggtcag cggttcgatc ccgcttagct ccaccaaatt tcaaaccctc gctgcaaagc
     6601 gggggttttt tgtctctgcc ttttgccgct tttgtaatac agtctacatc ctggcagtgc
     6661 cgtgtggtga atgcatcact gactgcaaaa acagcaacag gctggcccct gtttgcttcg
     6721 cgatgcgaat aaacttatta tttgtgtgcc tgaaaacccc gatcagtgag agtagtgtac
     6781 tcatgtttgt ggagcataac ctgataaaaa atatcaagat attcacacta gcgtttacgc
     6841 tcaccgtggt acttattcag ctatcccgtt taatttcgcc acttgctgtt atccattcca
     6901 attatatctt tctggcgtgg atgccactgt gcgtaatgct gtcaatcttg tttatctttg
     6961 gctggcgtgg tgtcgttccc attttatgtg ggatgttttg caccaatctg tggaaccttc
     7021 atctcacttt tttacagacc gccgtcatga tcggcagcca ggcgtttgca gtcctgtgtg
     7081 cctgtgcaat attacgctgg caattgggga cgcgttggcg ttatggattg accagccgat
     7141 acgtctggca acgtctgttc tggcttggtt tggtggcgcc gattggcatc aaatgcagca
     7201 tgtatcttgt gggaaatttc tttgattttc cgctaaagat atctaccttt ttcggcgatg
     7261 cggatgccat tttcacggtc gttgatttgc taagcctttt caccgcagtg ctgatttaca
     7321 acatgctttt ttactatctc acccgcatga ttgtaagtcc ccactttgca cagatattgt
     7381 ggcgtaggga tatcgctccg tcgttgagca aagagaaacg cgcatttacc ttaagctggc
     7441 tggcagctct tagcgtgctg ctacttctga tgtgcacacc gtatgaaaat gactttattg
     7501 ccggttactt ggtacctgtt ttctttatca tctttaccct cggtgtcggt aagcttcgct
     7561 atccgttttt aaatctcacc tgggctgttt caacgctttg cctgctgaat tacaaccaga
     7621 actttttgca aggggtacta accgaatatt cgctggcatt tattctcgca gtactgattt
     7681 cctttagtgt ttgtctgctc tatatggtgc gtatttatca tcgtagtgaa tggcttaacc
     7741 gccgctggca tttgcaggcg ctgaccgatc cgttaacgct cctacccaac tttcgtgcgt
     7801 tggaacaagc accggagcaa gaggcgggca agagtttttg ctgcctgcgc attgataatc
     7861 ttgagtttat gagtcgtcat tacggcttaa tgatgcgcgt tcactgtatc cgctcaattt
     7921 accgtacgtt gctgccgttg atgcaggaaa acgaaaagtt gtatcaattg ccgggtagtg
     7981 aactgctgtt agtgctgagc gggccggaaa cggaagggcg actccagcat atggttaaca
     8041 tcctgaatag tcggcaaatt cactggaaca ataccgggct ggatatgggc tatggtgctg
     8101 cctgggggca ttttgatgga aatcaggaaa ccctgcaacc cttgttgggg cagttaagct
     8161 ggctggcgga gcaatcctgc gcacatcatc atgtgctggc gctggatagc agagaggaga
     8221 tggtttccgg gcaaaccact aaacaggtgc tattgctgaa taccattcgc acggcgttag
     8281 atcagggtga tttgctgctc tacgcccagc caattcgcaa caaagagggt gaaggttatg
     8341 atgagatcct cgcgcgactg aagtatgacg gcggcattat gaccccggat aagtttctgc
     8401 cccttattgc tcagtttaac cttagcgcgc gttttgattt gcaagtgctg gaatccttgt
     8461 tgaagtggct ggcaacacac ccttgcgaca aaaaaggtcc acgattttca gtcaatttaa
     8521 tgccgctcac gctgctgcaa aagaatattg ccgggcggat tattcgtctg tttaagcgtt
     8581 attacatctc cccgcaggcg gtcattcttg agatcaccga ggagcaggcg ttttctaacg
     8641 cagaaagcag catgtacaac atcgagcagc tgcataagtt tggtttccgg attgcgattg
     8701 atgactttgg caccggatat gccaactacg aacggttaaa gcgtttgcag gctgatatca
     8761 tcaaaattga tggcgtcttt gtgaaagata tcgtcacgaa cacgctggat gcgatgattg
     8821 tgagatcaat taccgatctg gcgaaagcga agtcattgag tgtggtcgcg gagtttgtcg
     8881 agacgcaaca gcagcaggcg ctattgcata agctcggggt gcaatatctg caagggtatt
     8941 tgattggtcg cccgcagcca ttagccgatt aacaggcgta aaaaagccgg ggaattatcc
     9001 ccggcttttt tatgtatggt cttacagcac cagtgctgcg attgacgcag acagcacact
     9061 caccagggta gagccgtaaa ccagtttcag accgaagcga gaaaccacgt taccttgctc
     9121 ttcattcagg cctttaaccg cacccgcgat aatcccgatt gaagagaagt tagcgaagga
     9181 aaccaggaac acagagatga tgccttccgc acgcggagag agcgtggaag caattttctg
     9241 cagatccatc atcgcaacga actcgttgga aaccagtttg gtcgccatga tactgcccac
     9301 ttgcagtgct tcactggaag gaacacccat cacccatgca atcggataga agatgtaacc
     9361 caggatgccc tggaaggaga tgctgtagcc aaaccagcca gtcacggtgg caaacagtgc
     9421 gttcagcgcg gcgatcaggg cgataaagcc gatcagcatc gccgcaacga taatggcaac
     9481 cttgaaacct gccagaatgt attcacccag catttcgaag aagctctgac cttcgtgcag
     9541 gttggacatc tgaatatttt cttcactggc atcaacacgg taagggttga tcagcgacag
     9601 cacgataaag gtgctgaaca tgttcagtac cagcgccgca acgacgtatt tcggctccag
     9661 catggtcatg tatgcaccaa cgatggacat cgacacggtg gacatcgccg ttgctgccat
     9721 ggtgtacata cgattgcggg agattttgcc gaggatatct ttataggcaa taaagttttc
     9781 agactgaccc agaatcaggg agctgacggc gttaaaggat tccagtttgc ccatgccgtt
     9841 gactttggag agcaggaaac caattgcgcg gataatcacc ggcaacacgc gaatgtgctg
     9901 gagaataccg atcagtgcag agataaagac gattgggcac agcactttca ggaagaagaa
     9961 tgccaggcct tgatcattca tgctaccaaa gacgaagtta gtcccttcgt tggcaaaacc
    10021 gagcagtttt tcgaacattt cggagaagcc tttcacgaag cccagaccaa cgtcggagtt
    10081 caggaagaac cacgccagta acacttcgat aacaagcagt tgaataacat aacggatacg
    10141 aatttttttg cggtcgctgc ttaccagcag tgcgagaatc gcaacaacgg caagtgccag
    10201 tacaaaatga aggacgcggt ccatatttgc tccaaatatg aggcaggtta aatttccgtg
    10261 cacattctat gtaacatggt taaggaaaac gagatctgac acacactata atgacgtcct
    10321 gtgacaagct tcaaaaatag tgatctgtca tacatttctg ctatgttgtg tatggaagcg
    10381 gaaggttatg taaatgtgct aatattaaac ataaccataa cattcgagtc atctatcttt
    10441 tcgtagtcat aatcctggtc tatcagagaa atcatcacaa cccatttaaa tgaatttgat
    10501 aatcattctc atttaacgta gcatgaaaca tagcaaaggc tatgttttag aggcacaaga
    10561 tgacgaacta tcgcgttgag agtagcagcg gacgggcggc gcgcaagatg aggctcgcat
    10621 taatgggacc tgcgttcatt gcggcgattg gttatatcga tcccggtaac tttgcgacca
    10681 atattcaggc gggtgccagc ttcggctatc agctactgtg ggttgtcgtt tgggctaacc
    10741 tgatggcgat gctgattcaa atcctctctg ccaaactagg gattgccacc ggtaaaaatc
    10801 tggcggagca gattcgcgat cactatccgc gtcccgtagt gtggttctat tgggttcagg
    10861 cagaaattat tgcgatggca accgacctgg cggaatttat tggtgcggcg atcggtttta
    10921 aactcattct tggtgtttcg ttgttgcagg gcgcggtgct gacggggatc gcgactttcc
    10981 ttattttaat gctgcaacgt cgcgggcaaa aaccgctgga gaaagtgatt ggcgggttac
    11041 tgttgtttgt tgccgctgct tacattgtcg agttgatttt ctcccagcct aacctggcgc
    11101 agctgggtaa aggaatggtg atcccgagtt tacctacttc ggaggcggtc ttcctggccg
    11161 cgggcgtgtt aggggcgacg attatgccgc atgtgattta tttgcactct tcgctcactc
    11221 agcatttaca tggcggttcg cgtcaacaac gttattccgc caccaaatgg gatgtggcta
    11281 tcgccatgac gattgccggt tttgttaatc tggcgatgat ggctacagct gcggcggcgt
    11341 tccacttttc tggtcatact ggtgttgccg atcttgatga ggcttatctg acgctgcaac
    11401 cgctgttaag ccatgctgcg gcaacggtct ttggattaag cctggttgct gccgggttgt
    11461 cctcaacggt ggtggggacg ctggcggggc aggtagtgat gcagggcttc attcgctttc
    11521 atatcccgct gtgggtgcgt cgtactgtca ccatgctgcc gtcatttatt gtcattctga
    11581 tgggattaga tccgacacgg attctggtta tgagtcaggt gctgttaagt tttggtatcg
    11641 ccctggcgct ggttccactg ttgattttca ccagtgacag caagttgatg ggcgatctgg
    11701 tgaacagcaa acgcgtaaaa cagacaggct gggtgattgt ggtgctggta gtggcgctga
    11761 atatctggtt actggtagga acggcactgg gattgtagtt gaatgagcgt cgcatttggc
    11821 accattggcg gatgctgcgt aaatgcttta tccgccctac gcgttaaaga gctggtttgt
    11881 aggcctgata agacgcgcta gcgtcgcatc aggcattgtg ctccattggc gaatgtgttt
    11941 aatggcggtg tccatgtccg cggccttttc cacgatgatc gtcacgatcg cgccagcctt
    12001 cacgatagcc acgctcatac gctttacgct tatcccagcc acgatggtag ccattatcat
    12061 gacgccacca gcggttttta cgccattcat aattgcggtg ccagtagtca cggtcgcgcc
    12121 agtgaccacc gtcccagtaa ttaccgtaat gatcgcgatc gccaatttgt aatttgattg
    12181 atggcagtag ggtgatttca cctgcgtttg ccgcaagcgg ggtaaatgcc atcagggcgg
    12241 ccgccagaaa cagtgacctg aacattgtta ttctccttca cgatcgaagc cgtcagcggc
    12301 ctgttaacgc aatattacgg ggaggtaaag cgccgtatca tcgccataac tcttaaatca
    12361 taaaggagag cattttttgc taaacgttat cgtggggtgg ctgttgcttc ggacgattga
    12421 ccatcatcat cggcttacct gctaataaca gccatgccgg gagcatgacg atacagagca
    12481 gcggtagcaa tgtggtattg ggtacaacga ctgccgccat aaaaagactt aaccagccat
    12541 cacgtgttac caccagcacg atgccgagaa tagcgcagga aacggttatt gccgctggaa
    12601 cggcgggtac gtgctcatgc agcatcaacc ctaatgccac gccgacaaac actgccggga
    12661 agattcgccc accgcgaaag ccactggcgg cagcaacgac cagggcggca agttttatca
    12721 ccgccagcaa aaagtaatcg ctggtgctga aagcctgatt tgccaccatc tgctgcatct
    12781 catccagccc tttaaacagc gaaaccggtc caccaataac ccccagaata ccgagaataa
    12841 atccgccaat acccagcacg aacaccggat ttttcatttg atgcatcatc gcgtgcaacc
    12901 gtggtaagca ccatacggcg accatccctg ctgcgatggc aatcgccgcg acaattgcac
    12961 cgctgagaat atcggtcatt tccatctgtc cgtaatgcgc aatgggcagt gaaaaatgag
    13021 gatggaaaaa taatccggta gtaagtgcac cagctgctgc cgccattaac ggcgcaaaga
    13081 gacgatccca tagcggaact tcattactgc catttaaggt ttgcgaaaat atcaatgccg
    13141 ccgcaacagg tgtgccaaac agcgcgccga tggttccggc agaggctaaa atagtccact
    13201 ccattcggtt gacgcgcggt aacagacgag cgccaatggc gacggcaagg gcgatattga
    13261 cggtcatgat cggatgttcc ggccccaggc tgacgccgcc agcaagaccg agaattaatg
    13321 ctacgataag tcctggtagc gcagagggcg gaaccggtgc gccgatcagc ggttcacagg
    13381 cggggtctgg tccggcatga ccctggctga aacggataac caaccccacc gcaatacccg
    13441 ttagcgttaa tacaccgatg atccagagag gtgaatcctg ggctatcccc agagttcccg
    13501 gcagtcgttg ccagagcaaa ttctgtaata ccgaggcgat tttcatcacc acaatcagaa
    13561 taagactgga cgcaatccca attgccacgg cggggagcga taataacaac atggttctgg
    13621 ctcgcggatg gagcatgttc tattccttat gcggggtcag atacttagtt tgcccagctt
    13681 gcaaaaaggc atcgctgcaa ttggtgctga aacgataaag taattgtgtg acccagatcg
    13741 atatttacag ggagcctgcc tttccggcgt tgttgttatg cccccaggta tttacagtgt
    13801 gagaaagaat tattttgact ttagcggagc agttgaagaa tgacaaagta tgcattagtc
    13861 ggtgatgtgg gcggcaccaa cgcacgtctt gctctgtgtg atattgccag tggtgaaatc
    13921 tcgcaggcta agacctattc agggcttgat taccccagcc tcgaagcggt cattcgcgtt
    13981 tatcttgaag aacataaggt cgaggtgaaa gacggctgta ttgccatcgc ttgcccaatt
    14041 accggtgact gggtggcaat gaccaaccat acctgggcgt tctcaattgc cgaaatgaaa
    14101 aagaatctcg gttttagcca tctggaaatt attaacgatt ttactgctgt gtcgatggcg
    14161 atcccgatgc tgaaaaaaga gcatctgatt cagtttggtg gcgcagaacc ggtagaaggt
    14221 aagcctattg cggtttacgg tgccggaaca gggcttgggg ttgcgcatct ggtccatgtc
    14281 gataagcgtt gggtaagctt gccaggagaa ggcggtcacg ttgattttgc gccgaatagt
    14341 gaagaagagg gcattatcct cgaaatattg cgtgcggaaa ttggtcatgt ttcggcggaa
    14401 cgcgtgcttt ctggccctgg gctggtgaat ttgtatcgcg caattgttaa agccgacaac
    14461 cgcctgccag aaaatctcaa gccaaaagat attaccgaac gcgcgctggc tgacagctgc
    14521 accgattgcc gccgcgcgtt gtcgctgttt tgcgtcatta tgggccgttt tggcggcaat
    14581 ctggcgctca atctcgggac atttggcggc gtgtttattg cgggtggtat cgtgccgcgc
    14641 tttcttgagt tcttcaaagc ctctggtttc cgcgccgcat ttgaagataa agggcgcttt
    14701 aaggaatatg tccatgatat tccggtatat ctcatcgtcc atgacaatcc gggtcttctc
    14761 ggttccggtg cacatttacg ccagacctta ggtcacattc tctaaatcct tcctgttata
    14821 tcgggaggta actctcccga taatctttta aatcatacag ttaattaaat ttttctttgt
    14881 gtcccctcac aaggccgacc tgcgtcacac ttccgtacag cgggattaat tctccagtaa
    14941 atgcattatt tgtctggtaa cggcgatttg ttttgcacgt tcataatttc actcgtcaac
    15001 tatcactgaa cgaggaatta acgatgagta agaaactgat tgccttatgt gcctgcccaa
    15061 tgggcctggc tcacactttt atggccgctc aggcgctgga agaagcggcg gtagaagccg
    15121 gttatgaagt gaaaattgaa acccagggcg cggacggtat ccagaatcgc ctgacggcgc
    15181 aggatatcgc cgaagcgacc atcatcatcc actccgtggc agttaccccg gaagataacg
    15241 agcgtttcga atcacgcgac gtttatgaaa tcactttgca ggacgcaatt aaaaacgctg
    15301 cgggaatcat caaagaaatc gaagagatga ttgcctctga acaacagtaa tattcgacag
    15361 ggataattgt tatggccatt aaaaaacgca gtgcaaccgt tgtgcatggc gcatccggtg
    15421 cggcagcggc agttaagaat ccgcaggcct ctaaatccag cttctggggt gaactcccgc
    15481 agcatgtgat gtcagggatt tcacgcatgg tgccgacctt aattatgggc ggtgtgatcc
    15541 tcgctttcag tcagctgatt gcttatagct ggcttaaaat tcctgctgat atcggcatca
    15601 tggatgctct taatagcggg aaattctccg gtttcgacct ctccttactt aaatttgcct
    15661 ggctgtcgca gtccttcggc ggcgtgttgt ttggttttgc aattccgatg tttgctgctt
    15721 ttgtggcgaa ctctatcggt ggcaaactgg cgttcccggc tggttttatc ggcggcttga
    15781 tgtctaccca gccgacgcaa ctgctgaact tcgatcccag cactatgcaa tgggcgacct
    15841 cttcgccggt gccgtccacc ttcattggtg cgctgattat ttctatcgtt gccggttacc
    15901 ttgtgaaatg gatgaaccag aaaatccagt taccggattt cctgttagcg ttcaaaacca
    15961 catttttgct accgattctt tccgccattt ttgtcatgct ggcgatgtac tacgtcatca
    16021 ccccctttgg tggctggatc aacggcggta tccgtaccgt gctgaccgct gcaggtgaga
    16081 agggcgcact gatgtacgca atggggatcg ccgcggcgac agcaatcgac cttggtggcc
    16141 cgatcaacaa agcggcaggt ttcgttgcct tcagctttac cactgaccac gtattaccgg
    16201 tcactgcacg ttctatcgct atcgttattc cgccgattgg tctgggtctg gcgaccatta
    16261 ttgaccgtcg tttaaccggc aaacgcttgt tcaacgccca gctttatccg cagggtaaaa
    16321 ccgccatgtt cctggcgttt atggggatca gtgagggtgc gattccgttt gcactggaaa
    16381 gccctatcac cgccattccg tcgtatatgg tcggcgcgat tgtcggctca accgccgctg
    16441 tctggctggg cgcagtgcaa tggttcccgg agtccgctat ctgggcatgg ccgctggtca
    16501 ctaacctcgg cgtctacatg gcggggatcg cgctgggggc agtcataact gcgctgatgg
    16561 tggtgttcct gcgtctgatg atgttccgta aaggcaaatt gttaatcgat agcctgtaag
    16621 aaggacaatc ctgatgacat tactcgcttc gctgcgcgac tggcttaagg cgcaacaact
    16681 ggatgcggtg cttctctcct cacggcagaa caaacagccg catctgggga tctccaccgg
    16741 atcaggttat gtggtgatta gccgtgaaag tgcgcacatt ctggtggatt cgcgctatta
    16801 cgcggatgta gaagcccgca cgcaaggtta ccagctgcat ttgctcgacg cgatgcacac
    16861 gcttgccacc atcgtcagac aaatcattgc cgatgagcag ttgcaaacgc tcggttttga
    16921 aggccagcag gtgagttggg aaaccgcgca ccgctggcag tccgaactca atgcgaaact
    16981 ggtgagcgcc acgccggatg tgctgcggca aatcaaaacg ccagaagagg tggagaaaat
    17041 ccgccttgcc tgcgggattg ccgatcgtgg cgcagagcat attcgccgct ttattcaggc
    17101 gggaatgagc gaatgcgaga tagccgctga actggagtgg tttatgcgcc agcagggcgc
    17161 agaaaaagcc tcttttgaca ccattgtcgc cagcggctgg cgtggggcgc tgccgcacgg
    17221 caaagccagc gacaagattg ttgcagcggg cgagtttgtc actctcgatt tcggtgcgct
    17281 gtatcagggc tactgctctg atatgacgcg caccttgctg gtgaatggcg aaggggtgag
    17341 cgccgaatct catccgctgt ttaacgtcta tcagattgtc ctgcaggcac agctcgcggc
    17401 aatctctgta attcgccccg gcgtgcgctg ccagcaggtt gacgatgccg cgcgccgcgt
    17461 gattaccgag gccggttatg gcaagttttt cggccataac accggtcatg ctatcggtat
    17521 tgaagtccat gaagaaccgc gtttttcacc gcgggacacc acgacgctac agccaggtat
    17581 gttactgacc gtggagccag ggatttattt gccagggcaa gggggcgtgc gcatcgaaga
    17641 tgtcgtgctg gtcaccccgc aaggcgcaga agtgctctac gccgtgccga aaacagtgtt
    17701 gctcacggga gaggcataat ggatttatcg ctcttaaaag cgttgagcga ggcagatgcg
    17761 atcgcctcct cagaacagga agtacggcag atcctgcggg aagaagcgga tcgcctgcaa
    17821 aaagaagtgc gatttgacgg cctgggatcg gtgctgatcc gactcaatga gtcgacaggt
    17881 ccgaaggtga tgatctgtgc gcatatggac gaagtgggat ttatggtgcg cagcatctcc
    17941 cgcgaagggg cgatcgatgt gttgccggtt ggcaatgtac gcatggctgc ccgccagctt
    18001 cagtcggtgc gtatcactac ccgtgaagag tgcaaaattc caggcctgct tgacagcgac
    18061 cggcagggga atgacgtcag cgccatgcgc gtggacattg gcgcgcgctc ctgtgacgaa
    18121 gtgatgcagg caggaattcg tccaggcgat cgcgtcacct tcgataccac ttttcaggtt
    18181 ctccctcacc agcgggtgat ggggaaagcc tttgatgacc gcctcggttg ctatctgctg
    18241 gtgacgttac tgcgtgagct gcacgacgcc gaactgcctg cggaagtgtg gctggtggca
    18301 agttccagcg aagaggtggg attacgcggc gggcaaactg ccacccgcgc ggtgtcgccg
    18361 gatgtcgcca ttgtgcttga tactgcctgc tgggcgaaaa actttgatta tggcgcagct
    18421 aaccatcgcc agattggtaa cgggccgatg ctggtgttaa gcgacaagtc actgattgcg
    18481 ccgccaaaac ttaccgcctg gatcgaaacc gtggcggcag aaattggcgt accgttgcag
    18541 tcagatatgt tcagcaatgg cggcacggac ggtggggcgg tgcacttaac cggcactggc
    18601 gtacccacag tggtgatggg gcctgcaacc cgccacggac attgcgccgc atcgattgct
    18661 gactgccgtg acattttgca gatggagcaa cttttatctg cccttattca acgtcttacg
    18721 cgtgagacgg ttgttcaact gacggatttc agatgatctc ctgattaacc cggagcggtt
    18781 atgttaacga ttcaatttct ctgtcctttg cctaacggtc tacatgctcg tccggcgtgg
    18841 gaacttaaag agcagtgcag ccagtggcaa agcgaaatca cttttattaa ccatcgccag
    18901 aacgcaaagg ccgatgcgaa aagctcgctg gcgctgattg gcactggcac tttatttaat
    18961 gacagttgca gcctgaacat tagcggtagc gatgaagagc aggcgcggcg agtgctggaa
    19021 gagtatatcc aggtgcgctt tatcgacagc gatagcgtcc agccgacgct ggcagaactg
    19081 acggcgcatc cgctgccgcg ttcattaagc cgcctgaacc cggatttact gtacggcaac
    19141 gtgctggcaa gcggcgttgg cgtgggtaca ctgaccctgt tacagagcga cagcctcgac
    19201 agttatcggg tgatccctgc gagtgcgcaa gattccaccc tactggagca cagtctggca
    19261 acgcttgccg agcaactgaa ccagcaattg cgtgagcgtg acggcgaaag caaaactatc
    19321 ctcagcgccc atttgtcgct gattcaggat gatgaatttg caggcaatat ccgtcacctg
    19381 atggcagaac agcatcaggg gctgggggcg gcgatcatca gcaatatgga gcaggtttgt
    19441 gccaaacttt ctgcttctgc cagcgattat ctgcgcgagc gggtcagcga cattcgcgat
    19501 atcagcgaac agttgctgca tatcacctgg ccggaactaa aaccgcgcaa taatctggtg
    19561 cttgaaaaac tgaccattct ggtggctgaa gatttaaccc caagccagtt tttgagcctc
    19621 gatttaaaaa atcttgcggg catgattctg gagaaaaccg ggcgcacctc gcataccctg
    19681 atcctggccc gcgcttcggc gatcccggta ctgagcggac tgccgctgga tgcgattgcc
    19741 cgttatgccg ggcaacctgc agtgcttgac gcccagtgcg gcgtgctggc gattaacccg
    19801 aatgacgcgg tgagcggtta ttatcaggtc gcgcagacgc tggcggataa acgccaaaaa
    19861 caacaggcgc aagctgccgc gcagctggcc tattcccgtg ataacaaacg tattgatatt
    19921 gcggcgaata ttggcaccgc tctggaagcc ccaggcgcgt ttgctaacgg cgcggaaggc
    19981 gttgggctgt ttcgtaccga aatgctctat atggatcgcg acagcgcgcc ggacgagcag
    20041 gaacagtttg aagcgtacca gcaggtgcta ttggcggcgg gcgacaagcc gattatcttc
    20101 cgcacgatgg acatcggtgg cgataaaagc attccttacc tcaacattcc ccaggaagag
    20161 aacccgttcc tcggctatcg cgcggtacgt atttacccgg aatttgctgg cctgttccgc
    20221 actcaattgc gggcgatttt gcgcgctgcc agtttcggca acgcccagtt gatgatccca
    20281 atggtccaca gtctcgatca gatcttatgg gtgaagggtg agatccaaaa agcgatcgtt
    20341 gagcttaagc gcgatggcct gcgtcatgca gagacgatta cgctgggtat catggtggaa
    20401 gtgccgtcgg tgtgctacat catcgaccac ttctgcgatg aggtcgattt cttcagtatc
    20461 ggttctaacg atatgaccca gtatctgtat gcagtcgatc gtaataaccc gcgcgtatcg
    20521 ccgctgtata acccgattac gccatcgttc ctgcgcatgt tgcagcagat tgttaccgct
    20581 gcgcatcagc gcggcaaatg ggtaggtatt tgcggtgaac tgggcggtga aagtcgttat
    20641 ctgccgctac tgctagggct ggggctggat gagctaagca tgagtagccc gcgtattccg
    20701 gcagtgaaaa gccagcttcg tcaactggat agcgaggcgt gtcgggaact ggcgcgtcag
    20761 gcgtgcgagt gccgcagcgc gcaggaaatt gaagcgttac ttaccgcgtt tgcgccggaa
    20821 gaagacgttc gcccactgct ggcactagag aatatctttg ttgatcagtc ctttagtaat
    20881 aaagagcagg cgatccagtt cctgtgcggt aacctcggtg ttaacgggcg tactgaacat
    20941 ccgttcgagc tggaagaaga tgtctggcag cgggaagaga ttgttactac tggcgttggt
    21001 tttggcgtgg cgatcccgca caccaaatca cagtggatcc gtcattccag tatcagcatt
    21061 gcccggttgg tgaaaccggt tgactggcag tcagaaatgg gcgaagtcga actggtgatc
    21121 atgctgacgc tgggcgctaa cgaagggatg aatcatgtga aagtcttctc gcagctggcg
    21181 cgtaaactgg tgaataaaaa cttccgccag tcgctgtttg ccgcgcaaga tgcacaaagt
    21241 attctgacgc tgctggaaac ggaattaacc ttctgacgtt agccctgaaa acgtgcgcta
    21301 tactctcccg gcgtcagacc aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc
    21361 ggaaacccgc aacgctgcgc cacttcatga atcgacagat gatatttttg caaaatcatc
    21421 ctcgcttttg ccattcgtac ccagcgaaca tactcgataa aacgcattgt tccatgctga
    21481 gcaaacagtt ttgacagatg atttggcgtg atattaaaaa actgcgccac gctctcgcgg
    21541 gtgagcagct gggcataatt atcctgaatc cagttacaaa tgctgtgata gagaaaatcg
    21601 ccgcgcggtt gcgaattgcc cggcggcata ttcaccacgt tgcggcaaag atgcagcaaa
    21661 cttaacacca gtggctgaat gatattctgc tccagcggcg aacggctaag atgggtgagg
    21721 gcggtaagca tcgcttcacc ttcaccgcgt tgcaggctgg gaagctggat ctggcgagca
    21781 ggatgtaaca acgacgtggt gcggctatcg taaaacgata acccaagcca ggtcggtgcg
    21841 aacaccaggc ttaacagcag caccggtttg ttgttgaccg gtaaattagc cgcgcgtgct
    21901 ggaataaaca gcatttctcc ttccaccata cgttgctgct ttgcttccag cagattgccg
    21961 tattcgccgc gtagcacgat atccagacgg ggaaaatcaa tacataaact gcccactggc
    22021 aatgaggtag gctggctggc aaaccagacg cgccccagta gttgcgggtt aagcaccagg
    22081 ccgctgaaca gatcggcgaa aaattgttga tcggcgggca agcctggtgc tttcatgaaa
    22141 gttcccgatt aaagatgcat taactggcgg aattctttaa ctttgctgcg gctgaccggc
    22201 acttcaaaat ccagatcttt cagccgcaga atataggtgt tattaaacca cggttcgatc
    22261 tcgcggattt tgttcagatt gacacaaaat gagcgatggc agcggaaaaa atgcgacggc
    22321 ggcagcttgc tgcaaaactc ggtaatgttc atcggcatta cgtaggattc acgccgcgta
    22381 tagacaaagg tcattttctc atgcgcttcg gcgtaataga tatcgttaat gggcgtgacg
    22441 atgattcgct catctttcac cagattaatg gtgtcattct cacgcgttac cgtcgcggca
    22501 ggcgtactgc tggtctgctg ttgttgccag gctgcttcca gtttttgcag catcccggta
    22561 atgcgtgact cctgatacgg tttgagaatg tagtcaaacg cctccagttc aaacgcttct
    22621 accgcatgtt ctttccacgc ggtgataaac acaataaacg gtttatgggc gaactggctg
    22681 atgttttgtg ccagcaacac accatccagc gacggaatat tgatatccag aaaaatggcg
    22741 tcgacgcggt tatgctgcaa aaacttcaac acgtccagac cgtcgtcaaa ggtgccgaca
    22801 atctccatct ggctgtgctc tttaattagc cagctcagtt cctgttgtgc caggaattcg
    22861 tcttcaacaa tgatgacttt cacaatatca ctccggctca aagcaataag gtagcctgtg
    22921 aggcgactgg ggtgcgttgg ttaggaatgt aaaacgcaat ttccgttcct ggttccaggc
    22981 ggcggatatg cagcccctcg ccatacaata acttcacgcg atgatggaca ttcagcaggc
    23041 caattttatt gcccggcatt tcgttcgctt cgacccgctc aatcaccttt ggatcgatgc
    23101 cgtgcccggt atctcgcacc gcaatgcgta cccgattacc gcactcagca acgctgatgg
    23161 tgacaacgcc tttacctttg caaggctgga taccgtggac aatggcgttt tccaccaacg
    23221 gctggatcag caggctggga atgcagcaat tcacctcttc atcaatatca tagataaccg
    23281 tcagcttgtc gccaaagcgg gcctgctcaa tggcaatata gtctttaatt tgatacagct
    23341 cttttttgat atcgatttgc tcatcgtctt ttaattcaat gttatagcgc agatagcgcg
    23401 acaaattaaa gatcagctgg cgagcagtat ccggattcag acggattgac gatgaaatag
    23461 cgttcaaagc gttaaacagg aaatggggat taattttgct ttgcagggcg cgaagctctg
    23521 ccttatttgc catttcacgt agctgctcgg cgcgtgacac ctcaagttgc gtggagatga
    23581 tttgtgacag accgaccgcc atttcctgta atgacgaggt tatctgatgc gcgtggcagt
    23641 agtaaatttt cagcgttccg gtcacaacgc ctttctccca caatgggatc accagcatgg
    23701 aatgaatctc tggtgtgcgg tgggcttcat cattgttttt aatgatgatt ttcccgtaat
    23761 tcatcgcctg acgggtagtc gggctaatga agtcatcgcc attctgatag ttatgttcac
    23821 ccacgccaac gtaggccagc acatgatcgg tattagtcat cgccaccgca tcggcgtgaa
    23881 tgtcatcgcg gataatttcg cagaccttgc gtaatgactc gctattgaca tggcgaaaca
    23941 gcggcagcgt tttgttggcg atatccagcg ccagcttggc ctgccgcgcg gcgctggcct
    24001 ctttttctcc ctcaacactt tgtaccagca acacaataaa gccgatacag acgctgccga
    24061 ggatcattgg aatcccgatt ttagagacga tatcgatccc caatgcggta gttggtgccc
    24121 agacaatcac cagaatcatg gtcaacgttt cacacaacat gccgccgaga ataccgacgc
    24181 gccagcgctg tgctttgggg attttcaggt tgatccagcc cgatatacaa cccgccagaa
    24241 tgctggtgat aaagcagggg atcgccgtca cgccgccgat gtcaattaaa taacggtgaa
    24301 tacccgcaat cacgccggta atgatgccca cccacgggcc aaacagaatc ccgcctgaca
    24361 tcaccgcgat aatacgtacg ttcaccagcg agccttctac gggaacgccg gaccaggtgc
    24421 tgaacagggc gaacagcgag aaaatggcgg taacggcgag caactctttt ggggagtgcg
    24481 ccgacttgtg caacagttcg cgaaacaggc ggatacggat gagaaagaac aggcagataa
    24541 gcattaacgc tgcccgatcg aagaccgcca gcagcatgtt gaatatttcg tgcacgggtg
    24601 actctgaata aggattaaac ctctatgata ggtaacctga aggctgatga ccagcaggcc
    24661 ttttttgagg aaaaaccaca attttaaaag gggattgaaa gataaatggt gtattagcaa
    24721 tgcatctctt gataatgcac atcttgttgg taaatttatt gaaattataa
//
LOCUS       DAJOTB010000065        24726 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJOTB010000065 DAJOTB010000000
VERSION     DAJOTB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.065"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(61..1251)
                     /gene="bcr"
                     /locus_tag="OHN28_002545"
     CDS             complement(61..1251)
                     /gene="bcr"
                     /locus_tag="OHN28_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCQ2297926.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1279..1974)
                     /gene="rsuA"
                     /locus_tag="OHN28_002546"
     CDS             complement(1279..1974)
                     /gene="rsuA"
                     /locus_tag="OHN28_002546"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCQ2297927.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2124..3884
                     /locus_tag="OHN28_002547"
     CDS             2124..3884
                     /locus_tag="OHN28_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCQ2297928.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4009..4293
                     /gene="rplY"
                     /locus_tag="OHN28_002548"
     CDS             4009..4293
                     /gene="rplY"
                     /locus_tag="OHN28_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCQ2297929.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(4432..5439)
                     /gene="yejK"
                     /locus_tag="OHN28_002549"
     CDS             complement(4432..5439)
                     /gene="yejK"
                     /locus_tag="OHN28_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCQ2297930.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5621..5848
                     /locus_tag="OHN28_002550"
     CDS             5621..5848
                     /locus_tag="OHN28_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCQ2297931.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5868..7628
                     /gene="pbgA"
                     /locus_tag="OHN28_002551"
     CDS             5868..7628
                     /gene="pbgA"
                     /locus_tag="OHN28_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCQ2297932.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7703..7779
                     /locus_tag="OHN28_002552"
     tRNA            7703..7779
                     /locus_tag="OHN28_002552"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7737..7739,aa:Pro,seq:ggg)
     gene            complement(7890..8833)
                     /locus_tag="OHN28_002553"
                     /pseudo
     CDS             complement(7890..8833)
                     /locus_tag="OHN28_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            9153..9800
                     /gene="narP"
                     /locus_tag="OHN28_002554"
     CDS             9153..9800
                     /gene="narP"
                     /locus_tag="OHN28_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCQ2297933.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(9835..10887)
                     /gene="ccmH"
                     /locus_tag="OHN28_002555"
     CDS             complement(9835..10887)
                     /gene="ccmH"
                     /locus_tag="OHN28_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCQ2297934.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(10884..11441)
                     /gene="dsbE"
                     /locus_tag="OHN28_002556"
     CDS             complement(10884..11441)
                     /gene="dsbE"
                     /locus_tag="OHN28_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCQ2297935.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(11438..13381)
                     /gene="ccmF"
                     /locus_tag="OHN28_002557"
     CDS             complement(11438..13381)
                     /gene="ccmF"
                     /locus_tag="OHN28_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCQ2297936.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAARRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(13378..13857)
                     /gene="ccmE"
                     /locus_tag="OHN28_002558"
     CDS             complement(13378..13857)
                     /gene="ccmE"
                     /locus_tag="OHN28_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCQ2297937.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(13854..14063)
                     /gene="ccmD"
                     /locus_tag="OHN28_002559"
     CDS             complement(13854..14063)
                     /gene="ccmD"
                     /locus_tag="OHN28_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCQ2297938.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(14060..14797)
                     /gene="ccmC"
                     /locus_tag="OHN28_002560"
     CDS             complement(14060..14797)
                     /gene="ccmC"
                     /locus_tag="OHN28_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCQ2297939.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSILGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(14839..15501)
                     /gene="ccmB"
                     /locus_tag="OHN28_002561"
     CDS             complement(14839..15501)
                     /gene="ccmB"
                     /locus_tag="OHN28_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCQ2297940.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(15498..16115)
                     /gene="ccmA"
                     /locus_tag="OHN28_002562"
     CDS             complement(15498..16115)
                     /gene="ccmA"
                     /locus_tag="OHN28_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCQ2297941.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(16134..16736)
                     /gene="napC"
                     /locus_tag="OHN28_002563"
     CDS             complement(16134..16736)
                     /gene="napC"
                     /locus_tag="OHN28_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCQ2297942.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(16746..17195)
                     /gene="napB"
                     /locus_tag="OHN28_002564"
     CDS             complement(16746..17195)
                     /gene="napB"
                     /locus_tag="OHN28_002564"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCQ2297943.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(17192..18055)
                     /gene="napH"
                     /locus_tag="OHN28_002565"
     CDS             complement(17192..18055)
                     /gene="napH"
                     /locus_tag="OHN28_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCQ2297944.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(18042..18737)
                     /gene="napG"
                     /locus_tag="OHN28_002566"
     CDS             complement(18042..18737)
                     /gene="napG"
                     /locus_tag="OHN28_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCQ2297945.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(18744..21230)
                     /gene="napA"
                     /locus_tag="OHN28_002567"
     CDS             complement(18744..21230)
                     /gene="napA"
                     /locus_tag="OHN28_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCQ2297946.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(21227..21490)
                     /gene="napD"
                     /locus_tag="OHN28_002568"
     CDS             complement(21227..21490)
                     /gene="napD"
                     /locus_tag="OHN28_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCQ2297947.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(21480..21974)
                     /gene="napF"
                     /locus_tag="OHN28_002569"
     CDS             complement(21480..21974)
                     /gene="napF"
                     /locus_tag="OHN28_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCQ2297948.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            22382..22870
                     /gene="eco"
                     /locus_tag="OHN28_002570"
     CDS             22382..22870
                     /gene="eco"
                     /locus_tag="OHN28_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCQ2297949.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(23020..24666)
                     /gene="mqo"
                     /locus_tag="OHN28_002571"
     CDS             complement(23020..24666)
                     /gene="mqo"
                     /locus_tag="OHN28_002571"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCQ2297950.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT         6047 a         6980 c         6163 g         5536 t
ORIGIN      
        1 gatttgtggt tgacatgcat caacaaagga agccttttag cttcctcgtt gtgcaataga
       61 tcaccgtttt ttcggccgac tggcgtacag acagaagaga atggagctgg ttgcacagaa
      121 tgcaattgac caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa gtaatgcgcc
      181 aacaattgcc cctatgccga aacggaaggt tcctgccagc gaagatgccg ttcccgccat
      241 atggggaaac tcatcaagaa tgaccgccat cgcattggat gacaccatcg acacgcagcc
      301 cacaaacgcc gcaacgccaa ccaccagcga ccagaatccc agccccagca gtgcactgat
      361 gaccatccac gctgccataa taaactgtat ccacaacccc gagcggaaca tatttaacgc
      421 gccaatacgg cggacgaagc ggctgttaaa gatggtcatc acgaacagaa aaagaatgtt
      481 tagcgcaaag taataaccaa agttttccgg cgcgacgtgg ttgatttcaa tgtagacaaa
      541 cggtccggcg cttaagaatg agaacatccc ggcaaagctg aaaccgctgg ccagcatata
      601 actcaggaca cgcttatgac ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg
      661 aaaaggctgg cgacgctccg gtggtaaggt ttctttgatc agaaagaaga tcattgccga
      721 agccagaatc gccgccacgg cgaggatcca gaagatgtaa tgccagctca gccacaccag
      781 cacccagccg ccaactatcg gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa
      841 cgacatcatc cgtgagaact cttctttcgg gtagatatca cgcatcaaag cgttgataac
      901 cacgctggcc gcagccgcag ccagcccgtg gaagaaacgc atcacaatca gctgatcgat
      961 ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccaagaac
     1021 caccggctta cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa
     1081 gcccagaata taagtactga gggtcatctg cgtactgccc gccggtacac caaactgcgc
     1141 tgaaattacc ggtagcgcag gcagatacat atcaatcgac agcggcatca acatggccag
     1201 caggccaagg ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc
     1261 ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata
     1321 ttcaccgggg gctaaatcag catccagcgt aataccgcca atacgttcac gatgcagctc
     1381 aaccacatgg ttacccacgg cggcgaacat acgtttcacc tgatgataac gcccttcgct
     1441 gatggtcaaa cgaacctgcg ttggggtaat cacttccagc accgccggct tagtgagatc
     1501 tttttcgtta tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacggg
     1561 tgattccagc gtcaccagat aggtcttctc gcaatgatgg cgcggggaag taatgcggtg
     1621 cgaccactga ccatcatctg tcattagtac cagaccggtg gtatcgatat ccagacgccc
     1681 tgcagcgtgc agtttccacg ctaccggttc atcaagaaaa tagagcaccg ttgggtgatc
     1741 cgggtcgtcc gtggagcaaa catagccctg aggcttattg agcatgaagt aacgtggacc
     1801 gtgttgctgc gccagcgggt tgccatcgta agcgacatca tgttcaggga gcagtttgaa
     1861 cgctgcatta cggacgattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc
     1921 ggcaatagca cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt
     1981 gattttgcct gtaaaaaacg gaggtcgggt aatgacccga aaatctgaac tgttgtctgc
     2041 ccagtatagc ggtctaataa cttccctcaa gggaaaaaga tccgtggcat actattagca
     2101 gaataatcta cctacgcgag accatgattt ttacactccg cccatatcag caagaagccg
     2161 tggatgccac gctcaaccat tttcgtcgtc ataaaacccc tgccgtaatc gtgctgccca
     2221 ccggtgcagg taaaagcctg gtgatagcgg aactggcgcg gctggcgcgt ggtcgcgtgc
     2281 tggtgctggc acacgttaaa gaactggtgg cacaaaacca tgcaaaatat caggcgctgg
     2341 ggctggaagc cgatattttt gccgccgggc taaagcgcaa agagagccac ggtaaagtgg
     2401 tatttggcag cgtgcagtcg gtcgcccgta atcttgatgc ctttcagggt gaattttcgc
     2461 tgttgattgt cgatgaatgc caccgtattg gtgacgatga agagagccaa tatcagcaaa
     2521 tcctcactca cctgaccaaa gtgaatcccc acttacgcct gctggggctg actgccacgc
     2581 cttttcgact gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg
     2641 atgagaaagc ccttttccgt gactgcattt atgagctgcc gctgcgttat atgattaaac
     2701 acggctatct gacgccgcca gaacggctgg atatgccagt agtgcaatac gatttcagcc
     2761 gcttgcaggc acagagtaac ggactgttca gcgaagccga tctcaaccgc gagctgaaaa
     2821 aacagcaacg cattacgcca cacatcatca gccaaatcat ggagtttgct gcaacgcgca
     2881 aaggggtgat gattttcgcc gcgacggtcg aacacgcaaa agagatagtg ggattactgc
     2941 ctgccgaaga tgcagcactg attactggcg acacacccgg ctctgagcgc gatgtgttaa
     3001 ttgatgattt taaagcccag cgttttcgct atctggtcaa cgtcgcggta ctgaccaccg
     3061 gatttgacgc cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcggtgagtc
     3121 tttaccaaca aattgtcggg cgcggtctgc gcctggcccc cggcaagact gattgcttaa
     3181 ttcttgatta tgcgggtaac cctcacgatc tctacgcgcc ggaagttggt acaccgaaag
     3241 gcaaaagtga caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct
     3301 tttgggggaa aacgaccgcc gacgggacat tgattgaaca ctttggtcgt cgctgtcagg
     3361 gatggtttga agatgacgac ggacatcgcg aacagtgtga cttccgtttc cgttttaaaa
     3421 attgcccgca atgtaacgct gaaaatgata ttgccgcccg ccgttgccgc gagtgtgaca
     3481 ccattctggt tgacccggat gatatgttaa aagcggcgct acgactgaaa gacgcgctgg
     3541 tattacgctg tagcggcatg tctttgcagc atgggcacga cgaaaaaggc gaatggttga
     3601 aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc
     3661 ccgcccagcg aactgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca
     3721 tcccgctgcg ctggatcacc gccgccgatg tcctcgccca gcaagcctta ttgcgacacc
     3781 cggattttgt cgtcgcccgc atgaaaggcc agtactggca agtgcgtgaa aaagtgttcg
     3841 attacgaagg tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga
     3901 tgtataaggc gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt
     3961 acctgttgct gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc
     4021 aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac
     4081 aagttcccgg caatcatcta cggtggcaaa gaagcgccgc tggctgtcga actggatcac
     4141 gacaaagtca tgaacatgca agttaaagct gaattctaca gcgaagttct gaccatcgtt
     4201 gttgacggta aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg
     4261 aagctgctgc acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg
     4321 ctccggcggg gttttttgtc tctgcaaatt atgcctgatt acggtattgc tattttttgc
     4381 aggccagata aggcgttcac gccgcatctg gcattcagaa cacaaaaccg attaattgcc
     4441 gccagacgtc cggcgctgca attgatcgcg caagttcggc ggcgtgcctt taatggtcag
     4501 ggtatcggtt gctggatccc agaagatgcg ttcacctaac agcatcgcat caaagttaat
     4561 agtcaagcca ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc
     4621 cgggaagctc tcttccagtt cgtagccttt ttcagccgca aactccgtga agctcacttc
     4681 gctgacgcct gccagctctt tcgacagcga ttccagttca atctcttcac ctgcctgtag
     4741 ctgctcatta cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag
     4801 ttgtgcttcg gcagtgaaat catcgaccgc ttgcaacagc ccacggtttt gcgctttcgc
     4861 gtttaaccct tcgctggcac cgaggaaatc cataaagaaa tccgccactt tgcgccctac
     4921 tcgccctttc aggaaagtga gatagcgggt cgactctgga ttggtttccc actcggttaa
     4981 atcgatacgc gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc
     5041 gagattttcg ttaacacgca tactgctcag gttgctcaga actgccacca gcagatactc
     5101 caccgccaga tagcgatagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt
     5161 cgctaattcg tcacgcaggc gaccggttgc cgcgcggcta aatgccagaa agtcctcttc
     5221 gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta
     5281 agctttattt ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc
     5341 cgtcggttcc agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat
     5401 aagctggtgc agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc
     5461 gggcggtatt caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag
     5521 aaggcggcgt aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg
     5581 gtaatatgtt gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta
     5641 cagtgatgaa caggttgaac aattgctcgc agagctgctc aacatactgg aaaaacataa
     5701 ggctccgacc gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac
     5761 cagcattgcc ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc
     5821 ctctatcaac gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc
     5881 gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt
     5941 tcaatattct gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga
     6001 caacgcttgc tggtcgcatt tattcctacg taagcattat cggtcatttc agcttcctgg
     6061 tgttcgccac ctacttgctg atcctcttcc cgctaacctt tatcgtcggc tcccagaggc
     6121 tgatgaggtt tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgaca
     6181 gcgaagtctt tactcgtttc catctccatc ttaatcccat cgtctggcag ctggttatca
     6241 acccagacga aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta
     6301 ttttattgct tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc
     6361 gtcgtcggcg cttcgcgcgc ccgctggccg cattcttatt tatcgccttt atcgcctcgc
     6421 atgtggttta tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta
     6481 acctgccgct ttcgtacccg atgacggcac gacgttttct tgagaagcat ggcctgcttg
     6541 atgcgcaaga gtatcaacgc cgtcttattg agcaaggcaa tccagacgcc gtttccgttc
     6601 agtatccgtt aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtgctgttga
     6661 ttactgtcga tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt
     6721 ttgctgagca aaatatttcg ttcacgcgtc atatgagctc cggcaacact acagacaacg
     6781 gcatctttgg cctgttttat ggcatctcgc caagctatat ggacggcatt ttgtcgaccc
     6841 gtacgcctgc ggcgttaatt actgcgctta atcagcaagg ctatcagctg gggttattct
     6901 cgtcagatgg ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc
     6961 cgagtgtacg cacccaatcc gacgaacaga ccgccacgca gtggattaac tggctgggcc
     7021 gctacgcaca agaagataac cgctggttct cgtgggtctc tttcaatggc acaaacattg
     7081 acgacagcaa tcagcaggca tttgcacgga aatatagccg ggcagcaggc aatgtcgatg
     7141 accagatcaa ccgcgtgctc aatgcactgc gtgattctgg caaactggac aatacggtgg
     7201 tgattatcac tgccgggcgg ggtattccgc tgagcgaaga ggaagaaacc tttgactggt
     7261 cccacggtca tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta
     7321 ttaatgcgct gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg
     7381 tcagcacacc tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgcc
     7441 attactgggt taccgccgcg gataacgata cgctggcaat taccaccccg aaaaagacgc
     7501 tggtgctgaa caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag
     7561 atgaaaaacc acagttaagt ttgttattgc aagtactgac agacgagaag cgttttatcg
     7621 ctaactgatt aattataaat cagttagcaa aatatcttac ttgcaatcgg tgtggaaaac
     7681 ggtagtatta gcagccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg
     7741 tgtcgggggt cggaggttca aatcctctcg tgccgaccaa aaatcccaag aaaaccaacc
     7801 tattgcggtt ggttttttta tatctgcatt taattcgata aacagaccgc gacacatcac
     7861 ggcctgttta ttttctgtta tcagaacgtc cagaccacac ccgcctgagt tgtgctgctt
     7921 tcttcacggg agaaacggct gtcgatacca agcgttagcc ccgtcaatgt ttgctcaaag
     7981 ccagcgcctg catcagtggt cacgttgttg cggctgttaa ttgccgaact ccacatcgcc
     8041 gtatcgtaat taacgccttt tacgctacca agacgcccac gcacggtgtc cagttctgca
     8101 tcaaatacca gcggttgtgc tgccgccata ttcagcacat ccgtggttga agggggcatt
     8161 tgtgcgcgat tctctgcgag actccagcta tggttgccat tatccagcaa ggtatattca
     8221 tacgtactga tatcaacaac gcctccggca ttgcccaacg taaatgccgc atcaccgcgc
     8281 ccgttgttac cagtgtaagg ttatctcctg ctgccgggct ggcaccggtg tccgtcacga
     8341 atattttgaa atcacctgtt gctttaacct ccagtagccc tcccttttct ataacggcaa
     8401 agacggcaat cccatctttt tcaacaatta agcgccctcc tttaatcgtg ggatcagtcg
     8461 ccgcgccata aacctgtagc actccattat tgatcgtagt ctggtttacc gcgccaccca
     8521 tttgaacaat ttgataacca tcatttaata ctgagtattc tgcggtgcca tttatttcaa
     8581 tgtactgata tcccccgtta atttcagtat tactacttac accaaattct tatatatgct
     8641 gttctccgcc aggattgatt ttcgtattat tggcggccct acaaaccagc tggatatctt
     8701 tttccaggac aacaccatca actgtctcac catactcaac agttgatgcc aatgctatgg
     8761 gcgaatataa cgctgtagcc accagaacag atattgcgga acgacaaaga gaaacagaac
     8821 cagattggtg cattaagctt tcatcctatg aaattaattg ctgttaaaag cattggggac
     8881 agaaaatacc catagctcca tacccggagt cagtttttaa aaactgttta aagaaatgca
     8941 caagtattgt gattgatttt ttagttgttt ttcctgatga gaagctgatg caaaattccg
     9001 tctttataat aaagatgatg ccaaagcgaa cgacaaagtt gtagttttca ctacatgtcc
     9061 atacataaaa tggggtaaca ttcacgcgcc tggtagcgtt accaacgcta cgctcaaaca
     9121 taatgattct aataaaacct caggagacta ctatgcctga agcaacgcct tttcaggtga
     9181 tgattgtgga tgatcatcca cttatgcgac gcggtgttcg tcagttactg gagcttgatt
     9241 ctggctttga agtggtcgcc gaagcgggcg acggcgcaag cgcgatcgat ctggcgaata
     9301 gactggatat cgacgtgatc ttgctggatc tcaatatgaa aggcatgagt ggcctggata
     9361 ccctcaatgc cttgcgcagg gatggcgtta ccgcgcaaat tattatcctg accgtatcag
     9421 atgcctccag cgatgtcttt gcactgatag acgcaggcgc agacggttat ctgttgaaag
     9481 acagcgaccc ggaagtgtta ctggaagcga ttcgtgccgg agcgaaaggc agcaaagtct
     9541 ttagcgaacg cgtcaatcag tacttacgtg aacgtgaaat gtttggcgcg gaagaagacc
     9601 ccttcagcgt gctgacagaa cgcgagctgg atgttctgca cgagctggca caggggctgt
     9661 caaataaaca gattgcctcg gtgttgaata tttccgagca gacagtaaaa gtccatattc
     9721 gcaatctgct gcgtaaactc aacgtccgct cacgcgtggc ggccaccatt ctgttcctgc
     9781 aacaacgcgg ggcacaataa aaatagcccg atggcatcac catcgggcca ctttttattt
     9841 actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcga tcaccgcacg
     9901 gcgagtatcg ttggcaggta agagtttcaa catcatttcc cacgcggcaa cggcttcgcc
     9961 aaatcgttgc tgctcaaagg cattaaatgc atacatgctt aacacacgga tattgctatg
    10021 gtccgttctc accaactggc gcagcagttc gccgccgagg cggttgtcgt tgggatcaga
    10081 tgaacgtgtc aacgcttcag cgtatcccag tgcagcatcg ctgtttttcg gatcgaggcg
    10141 atacgcagtg gcgtatgcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg
    10201 gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag tacgcatccc
    10261 cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcggat ccagcgccct
    10321 gtccagtaac gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt
    10381 gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac ccggcagata
    10441 aacaacatat ccggcacgct taccttccgg cacgctttgt tctggaaacg cttccggcac
    10501 cacgcgtacc cgacgccgcg aacgggcgta tatgacccaa ccgccaatgc caatagccac
    10561 tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa
    10621 gttgccgtaa cgcgccacca tataatcgac aatctctttc ttacttttgc cttcctgcat
    10681 cagttcatac actttctggc gcaggtcggt ggcaatcatc gagttggaat cggcaatgct
    10741 gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg
    10801 tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg agatcatcag
    10861 catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt ctcccacagt
    10921 ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg atagcgaatg
    10981 atgccgttgc cgtcaataag gaacgtttct ggcgcgccat agacaccgag atccagccct
    11041 aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc tttcagccag
    11101 ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcggat gccctgcgca
    11161 gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaggt cgcccagacg
    11221 ttaagcagca ctggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg
    11281 tccagtgatt cgaggcgaaa cttcggcact ggcttgccaa tgagcgccga ttccagattg
    11341 gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc ggcaatcgcc
    11401 aggaagataa tcaacggaat taacaatacc ttgcgcttca tgcagcctcc ggcgcagttt
    11461 tctgcgggtt cacgcgctta cgatagcgag gatcaaacag acacagcagt ccgcccaacg
    11521 ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa agacgcacgg
    11581 cccacgcgcc gttttccagc tcttcaccga gcgccgcgta caggtcacgc gtgatgccac
    11641 cgtcaatcgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttctgcat
    11701 acagcactgt ttccggcttg ccatctcgcg ttacgccgat agtcgccaca ccgccacgcc
    11761 agtttggacc agtcacttct ttgacatcac ggaaggtgaa gcgatattca tgaatatcga
    11821 cgctatcgcc ggacttcatg cgcacatcac gctcaacgct atagttctga ctaaaagcaa
    11881 tgccaacaat tgtcactgcc agccccaggt gagctgccac catcccccaa taactgaagg
    11941 tggttttcgt gccgcgtgaa atacgccgcg cagcttccgc aattgccagc accgcaatcc
    12001 agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg
    12061 gcaacagcag cgacagcacc agcgtagaga tgaaggcgat aatcaacaaa ttgcggattt
    12121 tacgtgggcg atcgcgtccc cagcgcacca gagggccgac accaagcagt agcgcaaacg
    12181 gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca
    12241 gtcccagttg cttgtgcacc agcggcagca acgtccccag caacaccacc agcatcgcgg
    12301 ctaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta
    12361 cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca
    12421 gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg
    12481 ataccagcac gccggaacgc acgaggaaag tccccagcag acacaacgag aaggcactga
    12541 ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc gccagtgagt
    12601 gcatcagcgc agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga
    12661 accaccagcc gccccagccg agttcgtaat aggcccatgc ggaaccgagc acaatgccga
    12721 gcgtcaggaa gatccacgca gccagcgtcc acggacgagt aaaacgcgca taagtgctgt
    12781 ccagacgccc actcaacaaa gatgcaatgg caaaagcaaa cgccaccgag aaaccgacgt
    12841 accccatata gagcagaggc gggtggaaaa tcagccccgg atcctgcaac aacgggttaa
    12901 gatcgcgccc ttcaatcggg aaattcggca gcgtgcgtga gaacgggtta gaggtaaaga
    12961 gaatgaacag caaaaagccg acactgacca tccccattat cgccagtacg cgggcaacaa
    13021 tatccagcgg aatacgctga ctaaaaatcg ctaccgcaaa ggtccagccg ctcatcagta
    13081 gtacccacag caggagcgag ccttcatgcg cgccccaggt agccgccacg cgataccaca
    13141 ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg ttgaccacga
    13201 aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc caggcaaaca
    13261 agcgggaaga cgccatcatg cgcgcgtccc cgcgcgccac gccccatagc ggatacacag
    13321 acagcagcag cgcaattcct agcgccagac acagcagccc gttaccaatt tctggcatca
    13381 tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc
    13441 aacttctggc ggcgtgtagt tttcatcgtg tttcgccagc acttctttcg cgaggatatg
    13501 attgcctttt tccagttcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg
    13561 caaaatgcct tcgtaagaga catctactga accttcagcg tcgtaaatgg tgaaagttac
    13621 ttttagcgaa ttgggatcgc gctgcacact ccccggcata accatcccgc caacgcgcag
    13681 acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa tctcccccgg
    13741 cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc
    13801 cagccccgcc agcacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca
    13861 gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg cgccacgcca
    13921 cgcagaattg cgcgatgttg catcaccgag tgcacgacta aaaccaccag cggaataacg
    13981 gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc
    14041 caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg
    14101 gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc agcgtggcag
    14161 acaggagcag gaagcccaaa atcgaccagc gcagcggcga acgcatcgcc ggatcgatac
    14221 tttgctgcat tcgcgttgat ccctgatgca gggtgttcca ccactctacg gagtaatgaa
    14281 taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgc ccagccagac
    14341 gacggtcgtc gaaggcgtgc cacagggcaa tgacaccaac atacagaaac agcagcacca
    14401 gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt tttccccatg
    14461 cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca
    14521 ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc accgccattg
    14581 atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc
    14641 tatttccctg ctgataatca gccggagcaa agccgaaccc ccagatccag ccgacggtaa
    14701 gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc
    14761 gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca
    14821 aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg
    14881 acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc catcaacggg
    14941 caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag
    15001 cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac
    15061 gcccggcgca ccaagaaagc caagcgtagg ggttcccagc agtagcgtta acgccatcac
    15121 ttgccagcca taaacatcca ttcccagtag cattgctacc aggggcgaaa ggatgagtaa
    15181 cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg
    15241 taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg
    15301 ctcgagtgcc agcaaggatg aaagcagcgc ggcaacccag ataatacccg gcgcaatacg
    15361 cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatga caatcaggaa
    15421 gaaccacagc gggttggcga tttcggcgct gtggcgaaac gctacgcgca gctcaagacg
    15481 gaaaatgcgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg gcgaatttta
    15541 ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc ctgctccgta
    15601 tgctgcgcca tacgctgggt cagacgatcg acgccattaa cgtcaatcgc ggtaaaaggc
    15661 tcgtcgagga tccataacgt ggcacgggtc agccacagac gcgctaaagc gacgcggcgt
    15721 tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc
    15781 gccagggctt ccagacattg tgcggtatcg ccatcgcgat gataaaagtg cagattttct
    15841 aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg
    15901 tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc tgcatcaggg
    15961 cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg
    16021 atttgtaccc actctcctgc gttcagcgta aatgataaac cactaaataa ggttcgttca
    16081 tcccgctcgc aaagtaactc tctggcttca agcatactca cgcaataacc ctgttaaaaa
    16141 cctggctcga cttcacgcat gtcgggcagc ttgtgcgcta tccctttatg gcaatcaata
    16201 caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg
    16261 gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt gttgtccttc
    16321 atgcgccgcc actcattctg tgccatcgtc aggcgatgag cttcaaattt ctgcggcgtg
    16381 tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc
    16441 tttggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg
    16501 ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttacgcat ttcgtggcag
    16561 ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc gccccagaag
    16621 acgatgccac cgacaaaacc gatcaacagc agcgtcccca gcgccagacg gctgggggta
    16681 cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata
    16741 acctcttatt tcccgtaacc ttttgacggg gtaaaggtat tccccacgat tggcgcggta
    16801 tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc
    16861 actttgccgt cgctgtccat aaagtgagta ggactaatgc gcggcgcgcc agtggtgcga
    16921 taactttcga caccgtggca ctgcaagcag cggttggtat tggtcgttac ctgataacct
    16981 tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc
    17041 tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa
    17101 tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat
    17161 tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg
    17221 tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat
    17281 cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg
    17341 gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga tcggtggccg
    17401 caacggtaat cacacctttg ctacccagca cgccatacag tgcaccaacc ggacaaatgt
    17461 gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca
    17521 gcgcgccgct gccgaagccc attaccaggc tacgccccat cagggaaacg gggttgatcc
    17581 attcccagat gagcgtgcca gttaaggccg atcccaccag aatgatcacc aacagcacgt
    17641 agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaatcgtctg cgtaaccaat
    17701 ttgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcctg
    17761 ccagggcgta gagcacggtg ataatcaccg caccagtcag cgctaccgtg gcaggcagat
    17821 gaccgctggc aagactttgc agtgtcatca gcggatcggt cagtggaacg gtgtcgaata
    17881 acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccacgga ccgctcaaaa
    17941 acatccccag cacgaagaac tgacaaaggc gacgtaacac taaccaacgg tgactgcgcc
    18001 accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc
    18061 ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag tgacagcggt
    18121 aacaccttga ttgccggttg ttccagcacg cacacttttt cgcacttacc gcagccggta
    18181 caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggttcg cgtgttgcgc
    18241 tccagctcca gggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag
    18301 cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag ccccatccgc
    18361 gcgtcgtcga tcgattcgat ctcacgatcc agcgcaccgc tcgggcacac tttggcgcac
    18421 ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc
    18481 gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac
    18541 tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc
    18601 aaccgcacac cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca
    18661 gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt
    18721 ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag
    18781 tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac cagctgtgcg
    18841 gcgtcgaaga acggcatgta caccaggccc tgcggcggac ggttacgacc gcgcgtttcg
    18901 acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga
    18961 tcgcgcgctt tcgcatccag cgggtgaata aacagaaccg cttccgggaa ggcgcggtgc
    19021 agttccggta cacggcgagt catactgccg gtatgccagt gctccaggac gcgtccggta
    19081 gagagccaca ggtcgtactc ttcatccggc gcttccgccg caggttcgaa cggcagcgcg
    19141 aagatcaccg ctttgccatc cggtttaccg tagaacttgt agccttcgcc cgctttcacg
    19201 tacgggtcgt taccttcgct gtaacgccac tgcgtttctt taccgttcac caccggccag
    19261 cgcagaccgc gtgctttgtg gtaatcatcg aacggcgcga gatcgtgacc gtgaccgcga
    19321 ccaaaccatg cgtactcttc gaacaaccct ttttgcagat agaagcccag ctcgcgagat
    19381 tcgtcgttca gctgatcttc cgccagttcg gataccggga atttgctcac ttccggagtg
    19441 gcatacagaa cctcatacag cgttttgcca cgcagttccg gtttcttagc cagcagctct
    19501 tccggccaca cttcttcagt tttgaagcga cgggagaact gaactaactg ccagagatcc
    19561 gattttgctt cacccggtgc ctgtacctgc tgacgccaga actgagtacg gcgttcggca
    19621 ttaccgtaag ccccctcttt ctctacccac attgcggtcg gcaggatcaa gtccgctgcc
    19681 agcgcactga ccgtcggata tggatcggaa acgatgataa agttgcgcgg atcgcgccag
    19741 cccggcatac gctcttcatt aatgttcggt ccggcctgca tgttattggt acacatggtc
    19801 cagtaaacat tcagtttgcc gtctttcagc gcacggtctt gtgccaccgc gtgcagaccg
    19861 attttcgccg gaatggtgcc actcgggata ttccacttct tctcgcaaat atcacgatgt
    19921 ttctcgttag tcaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc
    19981 gcagtaccac acgcggaagg ctgcccggtc agggagaacg gaccgcaacc cggctgggaa
    20041 attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac accacgggta
    20101 tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc
    20161 tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcggcggt tttttccagc
    20221 gtatattcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa
    20281 cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc
    20341 gtcgcccctt tgcgcaggtt aacgtgtttg ctgaagaagt cctgatttat cgcattgttt
    20401 tgaatgatat agttggcgat atagttcagg atcaccaggt cagattgcgg cgtaaagatg
    20461 atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac cgccacggta
    20521 acgttctggt tagagagacg acggttagtg atgcgcgacc agaggatcgg gtgcatctcc
    20581 gccatgtttg cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc
    20641 atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc catacagtga
    20701 cgcgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt taaacagctt ggacgcggca
    20761 taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt
    20821 tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc ccaggtgatt
    20881 ggggtaaatt cgccttcttt atcatattta ccgtttttca tacgcagcag cggctgcgtc
    20941 aaacggtctt taccgtacat gattttgggc aggaaatagc ccttaatgca gttcaggcca
    21001 cggttaaccg gtgcgtccgg gtcgccctga caagccacca cacgcccctg ctgcgttccg
    21061 accagaacgc cgcaacccgt accgcagaaa cggcacggcg ctttatccca tttaatggct
    21121 tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc agccgccgca
    21181 gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat ggtgtttcct
    21241 caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc tctacgttgc
    21301 gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc accacaatca
    21361 actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc ggtaaaggcg ttcagttggg
    21421 tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa acttgccagt
    21481 tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc tgcacatgct
    21541 ccacatccgt tgcaaagttg gctattaagt tgcggctggt agatcccgga gagcgtcggg
    21601 cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc aactgactga
    21661 tacgccaggc acgcgtcccc aatggtgaac tgcaaatccc aggccctggt gtggcgcgga
    21721 gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca ctcgttattt
    21781 ttgaaattaa cgctcggata gctacccgcg ccgcgttgca gaatgttgtt ttcacaagca
    21841 ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc accggaccag
    21901 ggcggacgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc ccgacgggat
    21961 gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga gagcgaccaa
    22021 ataaaccgca taattaataa gccattttta tagccgctaa gatattaaag gatgtgtcaa
    22081 agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg cgggaagcgt
    22141 ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat atagcgatta
    22201 attcgccaga gatatttctg ctggtttgtt ttctcatcag aatttaacac taaaagagca
    22261 ggtaaaatta tctgaatgtt ctttaagtta ttcataaagc aaattaataa atctgatgaa
    22321 tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg aaagcaaaaa
    22381 aatgaagacc attctacctg cagtattgtt tgccgctttc gccaccactt ccgcctgggc
    22441 ggcagagagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg aaaaaggcat
    22501 gaagcgtcag gtgattcagt taactccgca agaagatgaa tctaccctga aagtagaact
    22561 gttaatcggt cagacgctgg aagtcgactg caatttgcat cgtctcggcg ggaagctgga
    22621 aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag ttagctcgcc
    22681 ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg tcaccgcgta
    22741 tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg tgtatacgcc
    22801 agacaatgtg gatgtgaagt accgtgtctg gaaggcggaa gagaaaattg acaacgcggt
    22861 tgttcgctaa actgctttga agtgctgcaa cctgtaggcc gaataaggcg gttacgccgc
    22921 atccgacatc taacgcccga gccggttgcc tgatgcgacg ctgaagcgtc ttatcagcat
    22981 acgccacatc cggcaaacca tgccggatgt ggcacatcat tacaacgcaa tatccgccac
    23041 ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg ctgcctgcgg
    23101 cttgtcgtat ttcagaccca gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc
    23161 tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg tagcctgcca
    23221 ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca taatcggcgc
    23281 ggcggttgac gcccctggcg atgcccccag gagcgcggca atggtccctt gctggtcact
    23341 gacgacttca gtaccgagac gcagtacgcc acctttatcg gcatcacgct tgataatctg
    23401 cacgcgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcctgcg gatagtactc
    23461 tttcaacact tcaaaacgat cctcttcact caacatcacc tgactcacca gatatttcac
    23521 cagatcgaaa ttatccagcc caacgtgcat catcggcatc acgttagagg tggtggtgga
    23581 actcatcaga tcccataatg aaccgttttt gaggaatttg gttgagaagg tggcaaatgg
    23641 cccaaacagc actacgcgtt taccgtcaag aacacgggta tcgatatgcg gaaccgacat
    23701 cggtggtgcg ccaacggatg ctttaccgta aaccttcgcc agatggtgat taaccacgtc
    23761 cgggttttcc gaaacaagga actgtccgcc caccgggaaa ccagcgtagt ctttcgcctc
    23821 cggaatcccc gattcctgta acagtttcag cgccgcaccg cccgcgccga taaagacaaa
    23881 tttggcgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtga cggtccagct
    23941 gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg agaagttaga
    24001 tttcttctgt aaggaggcga tcaactggcg ggtgatctcg ccatagttca catcggtgcc
    24061 aatttctgtg cgtgttgccg ctactttctg ttgcggatcg cgcccttcca tcactaacgg
    24121 tgcccactct ttgatctgcg cgtgatcttc agagtaacgc ataccgcgaa acagcgagct
    24181 ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg ttatcctcgc cccagacaaa
    24241 gctcatatgc ggaacggtat tgataaatga acgcggagta cgcagcacac cacgctcgac
    24301 ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg ttaatggcga ctgctttttc
    24361 aatactgata ctgccgtcgg cattttgcgg ggtgtaattg agttccatca gtgcagaatg
    24421 cccggttccg gcgttgttcc agccgttcga actctcctgc gcgacacctt ccaggcgctc
    24481 caccatggtc atcgaccatt caggctccag ctcgcgtaaa taggtcccca acgtggcgct
    24541 cataatgccg ccgccaatca acagtacatc agtttcctgc tcctcggacg ctttcgcttt
    24601 tgccgccatc gaaacggcat taagccccac ggccatcgag aagagcatgg cagtcacttt
    24661 tttcatcttg ttaatgcctt acttttagtc gctttattgc aggtgagatt tgcgcggcat
    24721 caacgg
//
LOCUS       DAJOTB010000066        24620 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJOTB010000066 DAJOTB010000000
VERSION     DAJOTB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.066"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..846
                     /locus_tag="OHN28_002572"
     CDS             <1..846
                     /locus_tag="OHN28_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2297951.1"
                     /translation="CIYQHLSISKAAESLYITPSAVSQSLQRLRAQFNDPLFIRSGKG
                     IAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISCTNNNMLIRCLR
                     QDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSN
                     KHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFINRKVSFRSSSLLTIINS
                     ISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLYISYNKASLNNLVFSRFIDR
                     LNDSF"
     gene            993..2213
                     /locus_tag="OHN28_002573"
     CDS             993..2213
                     /locus_tag="OHN28_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2297952.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            2186..2815
                     /locus_tag="OHN28_002574"
     CDS             2186..2815
                     /locus_tag="OHN28_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCQ2297953.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(2816..3976)
                     /gene="ybdL"
                     /locus_tag="OHN28_002575"
     CDS             complement(2816..3976)
                     /gene="ybdL"
                     /locus_tag="OHN28_002575"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCQ2297954.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTKMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4085..5173
                     /gene="hcxA"
                     /locus_tag="OHN28_002576"
     CDS             4085..5173
                     /gene="hcxA"
                     /locus_tag="OHN28_002576"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCQ2297955.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5183..5380)
                     /locus_tag="OHN28_002577"
     CDS             complement(5183..5380)
                     /locus_tag="OHN28_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2297956.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5493..7598)
                     /gene="cstA"
                     /locus_tag="OHN28_002578"
     CDS             complement(5493..7598)
                     /gene="cstA"
                     /locus_tag="OHN28_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCQ2297957.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7779..8192)
                     /gene="entH"
                     /locus_tag="OHN28_002579"
     CDS             complement(7779..8192)
                     /gene="entH"
                     /locus_tag="OHN28_002579"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCQ2297958.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8195..8941)
                     /gene="entA"
                     /locus_tag="OHN28_002580"
     CDS             complement(8195..8941)
                     /gene="entA"
                     /locus_tag="OHN28_002580"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCQ2297959.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8941..9798)
                     /gene="entB"
                     /locus_tag="OHN28_002581"
     CDS             complement(8941..9798)
                     /gene="entB"
                     /locus_tag="OHN28_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCQ2297960.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9812..11422)
                     /gene="entE"
                     /locus_tag="OHN28_002582"
     CDS             complement(9812..11422)
                     /gene="entE"
                     /locus_tag="OHN28_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCQ2297961.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(11432..12607)
                     /gene="entC"
                     /locus_tag="OHN28_002583"
     CDS             complement(11432..12607)
                     /gene="entC"
                     /locus_tag="OHN28_002583"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCQ2297962.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12796..13752
                     /gene="fepB"
                     /locus_tag="OHN28_002584"
     CDS             12796..13752
                     /gene="fepB"
                     /locus_tag="OHN28_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2297963.1"
                     /translation="MRLASLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIALFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13756..15006)
                     /gene="entS"
                     /locus_tag="OHN28_002585"
     CDS             complement(13756..15006)
                     /gene="entS"
                     /locus_tag="OHN28_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCQ2297964.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLSVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            15117..16121
                     /gene="fepD"
                     /locus_tag="OHN28_002586"
     CDS             15117..16121
                     /gene="fepD"
                     /locus_tag="OHN28_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCQ2297965.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16118..17110
                     /gene="fepG"
                     /locus_tag="OHN28_002587"
     CDS             16118..17110
                     /gene="fepG"
                     /locus_tag="OHN28_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCQ2297966.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17107..17922
                     /gene="fepC"
                     /locus_tag="OHN28_002588"
     CDS             17107..17922
                     /gene="fepC"
                     /locus_tag="OHN28_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2297967.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17919..19052)
                     /gene="fepE"
                     /locus_tag="OHN28_002589"
     CDS             complement(17919..19052)
                     /gene="fepE"
                     /locus_tag="OHN28_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCQ2297968.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GQGKAIIVILSALVGGMVACGGVLLRHAMASRKQDAMMADHLV"
     gene            complement(19299..23180)
                     /gene="entF"
                     /locus_tag="OHN28_002590"
     CDS             complement(19299..23180)
                     /gene="entF"
                     /locus_tag="OHN28_002590"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCQ2297969.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            complement(23177..23395)
                     /gene="ybdZ"
                     /locus_tag="OHN28_002591"
     CDS             complement(23177..23395)
                     /gene="ybdZ"
                     /locus_tag="OHN28_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCQ2297970.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23398..24600)
                     /gene="fes"
                     /locus_tag="OHN28_002592"
     CDS             complement(23398..24600)
                     /gene="fes"
                     /locus_tag="OHN28_002592"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCQ2297971.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     AIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTIGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
BASE COUNT         5882 a         6926 c         6239 g         5573 t
ORIGIN      
        1 tgtatttatc aacatctcag cattagtaaa gcggcagaat cattatatat aacaccctct
       61 gctgttagtc agtcgttaca acgtttacgt gcgcaattta atgacccgtt attcattcgc
      121 tcagggaaag gtatcgcgcc gactacaaca gggttaaacc tgcatcacca tcttgagaaa
      181 aatctcagag gccttgaaca aacgatcaat atcgttaata agtctgaact aaagaaaaat
      241 tttattatct acggtcctca gcttatttct tgtactaata acaacatgct aatccgctgt
      301 cttagacaag atacttcaat cgatatagaa tgccatgaca tactgctttc agctgaaaat
      361 gccgaagaac tgttggttca acgcaaaact gacctggcaa ttacgctgca accagtaatt
      421 agtcgctctg tcatctgcat gccgttacac actattcgca attcccttat atgtagcaac
      481 aaacatcctc gtattactga taccagtaac tatgaacaaa ttatcgccga agaattcacc
      541 ctgttgatat ctaaatcagc agggattgac gaaattcaaa tggacataga tgaacgtttt
      601 ataaaccgta aagtttcatt tcgcagttct tctttgttga ctataattaa tagcatttct
      661 gttactgact tattaggcat tgtgcccacc gaactgtacg atttacatcg tgactttttg
      721 aagttaaaag aaataaaact ggaacaacct cttcctgcgg ttaaacttta tatttcctat
      781 aacaaggctt cgttgaacaa ccttgtcttc tctcgattca tagatcgttt aaatgatagc
      841 ttctgattca ttaccactga caaataatat attacgcatc aattaattca ttgtgtgaat
      901 ataaaataat gtgctctatc ttgcttcgtt tatcctataa ttagcggatt catctcacga
      961 gtcaccccgt aacgttcaga gagcgttttt agatgtctat ttataaaatc ccgcttccac
     1021 tgaatattct ggaagctgca aaagaacgta ttacctggac acttaatacc ctaccccgta
     1081 tctgtgtttc tttttcgggt ggtaaagatt ccggattaat gctgcatctg acagctgaaa
     1141 ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggaa gcgcagttct
     1201 catgcactat taactatgtt cagtcattac gtgagtttta cgccgatgtc atcgaagagt
     1261 tttactgggt tgcgctcccg cttacgacgc aaaattccct ttctcaatac caacccgaat
     1321 ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg
     1381 atcctgactt tttctccttt taccagcctg gcatgacctt cgaacaattt gtgcgtgagt
     1441 ttgctgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc cgtgcagatg
     1501 agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt gccgatgata
     1561 agccctggac caccgccgca ccaggcggtc atagctggta catttacccc atctacgact
     1621 ggaaagtggc tgatatctgg acctggtatg ctaaccatca acagctctgt aacccactgt
     1681 ataatataat gtatcaggca ggcgtccctc tgcgtcatat gcgaatttgc gaaccttttg
     1741 gcccggagca acggcaagga ttatggctct atcacgttat cgaaccggat cgctgggctg
     1801 ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga catgataatc
     1861 atttttatgg tcatcggaaa atcctcaagc cagaacattt agactggcaa gaatatgcat
     1921 tattgctgct caatagcatg ccggaaaaaa cagcggagca ttaccgcaat aaaatcgcca
     1981 tttatttgca ctggtatcag aaaaaaagca tcacggttcc acaaactcag caaggggaca
     2041 ttggggcgaa agatatcccc tcctggcggc gaatatgcaa agttttactc aataacgatt
     2101 actggtgtcg ggcattatca tttagcccta cgaaagcgaa gaactatcag cgttataacg
     2161 aacggataaa aggaaaacgt aaggaatggg ggatactatg caacaacgat taacgcagga
     2221 tttaacccaa tttctcgcca gcttgccgga agatgatcgc atcaacgcga ttaatgaaat
     2281 ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg
     2341 ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg caccacccga
     2401 aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca ttgtggtcac
     2461 gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg aaattggtaa
     2521 aggctcatca tcgttaaaat tacgcttaaa aggttatttg ccggtgacct gtctggaagg
     2581 tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg gacgtcacca
     2641 aatcactgcc atgtcagaaa tcgtccgaga actcagccag ttgggatggg acgataataa
     2701 aattggcaaa gagctgggaa tggacagcga cgaagtgtta cgcctgaagc aaattaacgg
     2761 cctgcaagag ttgtttgccg accgtcaatt ttcccgcgcc tggacggtta aataactaca
     2821 gctggcgcag gcgttctgct gctgccagca acgtcgattc cttcttggca aaacagagac
     2881 gaatcagttt atgtgggaag ggatcggcgc aaaacaccga cagcggaatc gccgctacgc
     2941 cgtgctcccg cgtcagccac tggcaaaact caacatcatc catcgtagat acggcgctgt
     3001 aatccaccag caaaaagtat gtaccttcgc acggtaaaat ctccagccga ctttcattta
     3061 aggcattcac cagaatatcg cgcttctggc gataaaagtc cggtaatgca agataatgct
     3121 caggttctgc acgtagcata tcggcaagcg ccagttgtgc cggggtattc accgaaaagg
     3181 tcagatactg atgcactttg cggatctcgg cgctgatggg cgcaggcgca acgcaataac
     3241 ccactttcca gccggtcata tgataggtct taccaaacga tgacaccgcc accgctcgct
     3301 cacgcaattg cggatgcgcc agcacgctgg catggccctg ttgtgaaaaa ttgatgtgct
     3361 cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc cacagagcgg
     3421 cgaaatcagt ctgctgccag acagttgcac tggggttatg cggggtgttg aggatcacca
     3481 gtcgggtgcg ctcgcttaac aatgcggcaa attcctgcca gtcaacgcga aaatgcggcg
     3541 gttgcagtgc aatacgtttc actattcccc ccgaaagtgc gatggcgggg gcgtaactgt
     3601 catagctggg atcaaaacaa atcacttcgt cgccattgcg caccaatgcg gttatcgccg
     3661 cgtataacgc ctccgtcgcc cctgccgtca cggtgatatt gctgtcgaca tccggttgat
     3721 agccatacag acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca
     3781 tgggtgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc
     3841 gcggaccatc aaaatcagga aagccttgcg aaaggttaat cgcctggtgt tgctgcgcca
     3901 gcgcgctcat cttggtgaaa atagtggtgc caagttgtgg aagtttgctt tgtggaatca
     3961 gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg cactataaca
     4021 cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat aaggacaaca
     4081 caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact tttcacatcc
     4141 cggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc gcgcggtgtg
     4201 gatctacggc gaacgcgcca ttgctgcggc acaaaccaaa ctgccgcctg cgtttgaact
     4261 gccaggggta aagcatattt tgtttcgtgg tcattgcagc gaaagcgacg tgcaacaact
     4321 ggcggcggag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg gcgcactact
     4381 cgacaccgcg aaagccctcg cccgccgtct cggtctgccg tttgttgccg ttccgacgat
     4441 cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg ccggacaagc
     4501 gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac cggagattat
     4561 cctcaatgcg ccacaagaat atctactggc ggggatcggt gacacgctgg cgaaatggta
     4621 tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc ggctgggaat
     4681 caataatgcg caggccattc gcgacgtctt gttaaacagt agcgaacagg cgcttgccga
     4741 tcagcaaaat cagcaattaa cgcaatcatt ttgcgatgtg gtggatgcga ttattgctgg
     4801 tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg cccatgccgt
     4861 gcataacggt ctgaccgttt tgccgcaaac cgagaagttt ctccacggca ccaaagtcgc
     4921 ctacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg cgcaattaac
     4981 cggggcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag tggatatcaa
     5041 taatcaggtg gagatcgaca aaatgattgc ccacacccta cgtccggtgg aatccattca
     5101 ttacctgcca gtcacgctga caccagatac cttgcgtgca gcgttcgaaa aagtggaatc
     5161 gtttaaagcc tgacgtacaa atttagcagc agcgcgcgcc gccttttcca ccatagcgcg
     5221 catcctgccg ctcacggaaa aactcctcgt aggtcatcgg cgtttgatcg ggatggttaa
     5281 cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagt ttcgccgcct
     5341 gacctaaata ttttccggct tttgccagtg aatcaaacat ctaaacctcc ggtaatacaa
     5401 atgccctcct gaattaagag ggcttaattg ctatcaacac gctcgggctg agagtaaggg
     5461 gaacgttcag cacatccccc ctcactctga ctttagtgcg cgcctttcgc ctgcgccacg
     5521 atctcctcga cattttccgg catcggctca tatggcgttt ctttcgccgt tggcttcggc
     5581 tctttcaatg ccgccagtgc cgtcttaatc gagaacagcg ccagaaccac cacgaccacc
     5641 ataaagaaga tggttaaccc ggcatcaaga cggttgttga acaccagttg cgccagttgt
     5701 gactcagtat actgcgatgg aatattgccg ctgtcgatca ttgcctggaa cttgttagca
     5761 atcgccagga atccgacttt cgcatccggg ctaaacgctt tctgccagcc tgcggtcagg
     5821 gtacaaatca gcagccaggc cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc
     5881 attttgaaca gcaccacagc acaaagcatc agcgccatcc ctgccagcat ctggttggca
     5941 ataccaaaca gcggccacaa ggtgttaata ccgcccagcg gatcgacctc gccctgatgc
     6001 aggaagtagc cccacgccag cacgcacagc gccgttgcca gcaggttagc aggcagtgaa
     6061 tcggtccgtt tcaggcccgg agacaccacg cccagcagat cctgcaacat aaagcgcgca
     6121 gcacgcgtac ccgcatccac cgccgtcaga ataaacagtg cttcaaacag aatggcgaag
     6181 tgataccaga acgccacatc catcatgccg cccagcgcac cgtgcagaat gtaggccatc
     6241 cccaccgcca gggtcggcgc accgcccgca cgggaaatga tcgactgttc acccacttcg
     6301 ctggcaatct ggtttagcgt atccggggta atcgcaaagc cccagctact caccacctgc
     6361 gcggcagaag caaccacatc cgccgtccct gccggagcca gcaccgccat cgggctgttc
     6421 atggcgaagt acacacccgg atcgatgata caggcggaaa ccagtgccat aatcgccaca
     6481 aaggattcca ttaacatccc gccgtagccg ataaagcacg cctgcccttc gttcgccagc
     6541 atcttcggcg tggtgccgga agagatcagc gcatggaagc cagacaccgc gccacaggcg
     6601 atggtgataa acaggaacgg gaacaggtta ccggtccata ccgggccagt gccatcgaca
     6661 aatttagtta gcgcaggcat ggtcagcgtc gggcgcataa ttaaaatgcc caccgccaga
     6721 ccgacgatag tcccgatttt caggaaggtt gagaggtagt cacgcggagc cagcagcaac
     6781 cacaccggca gcaccgccgc cacaaaaccg tagcccacca gcatccaggt cagctgcacg
     6841 ccggtaaagt caaagtacgg tgcccaggtc gggctttctg ccacccagcc gccagagata
     6901 atggcgaaaa tcaggaacac cagaccaatt accgacactt cgccaatacg ccccggacgc
     6961 aaatagcgca ggtagatccc cataaacagc gccagcggaa tggtgaacgc aacggtatac
     7021 gttccccacg ggctatgagt cagggctttc accacaatca tcgccagcac ggcaaggata
     7081 atgaccatga tcataaagca ggccaccagc gctatcaccc cggcggttgg tcccatctct
     7141 tctttgacca gctcacccag cgagcgaccg tcacggcgcg tagaaacaaa cagcaccatg
     7201 aaatcctgca ccgcaccggc gagaaccacc ccggcgagca gccagatcat ccctggcagg
     7261 taacccattt gcgccgccag taccggccca accaacggac ctgctccggc aatggccgca
     7321 aaatggtgac cgaacagcac ttttttgtcc gtcggcacat agtccagccc gtcgttatgg
     7381 cgcaccgctg gcgtcatacg cgtcgggtca accgccagca catttttggc gatatacagg
     7441 ccataaaaac ggtaggcgat tagatagata cagaccgacg ccaccacaat ccacagcgcg
     7501 ttgatctgtt ccccacgatt taaagcaatg tatcccagag caaatgctcc cattacagcg
     7561 agcactgtcc agacgaggta tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc
     7621 catagagatg ttacattttg tttctataac atctttgtac aatttaacaa cacagcaacc
     7681 atgtaaatcg gaattgaaac caatatttaa cttaacaatg ttaactcgat cactccgtta
     7741 accgagtttt cctgcaatct catttaattc tgtcgggctc atcccaaaac tgccgtaccc
     7801 agccgacagg tgcagcagcg ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga
     7861 ttttgtcgtc caagatgcag cggctggcag acaccgcgca cctttccctc agacaccggg
     7921 cgatgatgtg tggcgttaag ttccgtgccc accacacact gtccgtcgcg ggtcatcata
     7981 aatccggcca tcgatcccag cgtttccgcc agcgccgccg acgcgccgcc atgcagcagg
     8041 ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg cttcgagtac atcatcgccc
     8101 agacgggtat acacaattcc cagatgcgcc accattgtgt tatcgctggt ggcgttcagc
     8161 tcgtcgagcg ttaaatgacg tttccagatc atgcttatgc ccccagcgtt gagccgccat
     8221 cgaccacaat atcctgtagg gtaatatggc tggcgaggtc agaggcgagg aacaaaatcg
     8281 tgttggcgat ctcttgtgga caggcgattt tccctagcgg aatgccgagt ttaaactgct
     8341 cgccaaagcc gcgaatacgc tgctcttcgg cgtcatcgct cacccacagc gtgcgttgca
     8401 tatcggtgtc ggtggagccc ggcgaaacca cattacagcg cacgccgcta cccgccagtt
     8461 ccagcccgac gctcaacgcc aggcttttca gcgccgcttt cgacgcgcca taagcactca
     8521 taccaatacg cggcgtgtgt gcggcgtcgg acgcaacagt aacaatcgcc ccgccccgct
     8581 gacggcgaaa ctggttcatg gtctgctgaa acaggttaaa cgcaccgccg acgttgaccg
     8641 caaaagtctg ctgccagtcc tctttactga gctgatcggt cgcgcccatg cgcaaaattc
     8701 ccgccgcatt gaccagcacg tccagtcgct ccgtttcagc taacagtcgc tgacatacct
     8761 gcgccacctg cgcagcatcg gcaacatcca ttacttcggt cgcaaaagga tattgctcct
     8821 gactaaacgc ttgatcaaaa cctgtaactt tcgcccccgc ctcaacaaat gccagcgccg
     8881 tggcgtagcc tatacctttc cccgcgccag ttacccagac atttttaccg ctgaaatcca
     8941 ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag
     9001 catgacaaag tcgatatcgc catgcacttt gcgccagcgc gccgccagcg ccatcatgcg
     9061 caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg aagggttcat cggactcgtc
     9121 cagcaacggc aggatcacct cacgcagcgc cgctttgctg gcggggatag gtgctggcag
     9181 caattcttcg gtcatcacca cccgaccaga acgtccggcc acatatttca gcgacatcaa
     9241 atgctcgtca cggctgaaat cggccagcgc atccgccacc ataaacggtt taatatcgcg
     9301 cataaatgcg tccgttgcgg tggtcataca gccaatgtgg gcatataccc cggtaataat
     9361 cagctggtta cgtccactct ctttgagcat ttgctccagc ggagaacgat gaaatgcgct
     9421 gtagcgccat ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt
     9481 ttgctgttcc ggcgaacggg tcaggccagg cccccacata tcattcaaca gcgcccgatc
     9541 ttcatcgctc tgctctttcg gctgggcggt gtaataaacc gggatattgt gctgtttgca
     9601 gtagtcgcgt agtgccgcaa tattcgcgat cacctgctcc atcatcgggc agttctcacc
     9661 ccagaagctg acaaaatagt cctgcatatc atggattaac aacgcggcac gttgcggttc
     9721 aaaggcccag tcgactttat tctgcggaat atcgtgaggc tccggcagtg cgtaagcctg
     9781 taattttgga atagccatcg tgttctctcc ttcaggctga tgtgcgtgac gccagccact
     9841 gacgtaattg ttttttatcg actttcccga ccgccgtcag cggaagtgaa tctacacact
     9901 ccacgcgatc cggtaattta aattcggcaa taccctgttc acgcaggaaa cgacgcacct
     9961 gcaccgcgcg cagcggctct tttaccacca gataagcaca gcttttttcg cccatcagct
    10021 catcttccat gctcaccagt gcggcgtaga tcaccgccgg atggcgcagc agcaggtttt
    10081 cgatctcttc ggcagcgatc ttctcgccgc cacggttgat ctgatctttc tcgcgcccct
    10141 gcacagtgat gtaaccctct ggatcaatag agatcagatc gccggaacag taaaaaccgt
    10201 tggcatcaaa ggcgctggca ttgtgctgcg ggcttttgta atagccacgg aaagtgtacg
    10261 gcccgcgcgt catcaggcgt ccaacttccc cttgcggcag tggatttcct tcggcatcgg
    10321 caacccatac ttcatcgtcc gggcacattg ggtaaccctg ggtatggata attttctccg
    10381 cactatcatc aagacgggtg tagttcacca gcccttccgc catgccaaac acctgctgca
    10441 actggcagcc aatctcagcg ggaatacgcg ccgcaagcgt ggcagaaaga cgtgcgccgc
    10501 cgacctgtaa cagtttcagc gaggcaagct gcgcccggct ttcgccttca gccagtgcct
    10561 gcaaccacag gctgactgct ggcggcacca gcgcggtgac gttcacctga tgtttttcaa
    10621 tcaatgggaa gcaaagcgtg gcgctggggt cggcagccag aacgacagtg ccgccagcga
    10681 gaaatacccc caacgatccc ggtgaactca tggcgtagtt atgagccgcc gggatcgcgc
    10741 acaggtagcg cgtctgttgt gtgaactgac aaatctcgac gctacgacgc acgctgtagt
    10801 agtagtcgtt atgagtgcgc gggatcagtt tcggagtgcc ggtggtgccg ccggaaagct
    10861 ggaaataggc cacttcatca gcaggagatg gcgtggcagt aaaatcctca gccggatggt
    10921 taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgag cagctgcacc acgcgaatgg
    10981 aagaatgctc tgcaacaaat gtgttgagga aatcatcccc gctaaatagc gcatgttggc
    11041 gatcggcaat cagtaacgcc ggttcaatct ggctggcata ggcgttcagt tcactacgct
    11101 gatggctgaa cagcgccagc accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa
    11161 tgtaaagttc agcgacgtta cccagttgta ccagcgcggt ttcaccaggt ttaatgccct
    11221 gacggcgcaa actacaggcg aggttatccg ccgcctgatt cagctcccgg taactcaact
    11281 gccactcgcc gtcgatcacc gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg
    11341 tcagtggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac tcttccggcc
    11401 atcgggtgaa tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg
    11461 tagaaagttt gacgcctgtt tcgcgccact cacccaacgg tgaggacgca ggcacaatcc
    11521 ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg atggtcacca
    11581 cccattcacc gttaccttcg ctgtcacacc aacccacaat gccgccaaac agttcgcggt
    11641 cgaacggctc cagttcagca ataacctgag tcgcggccag atgcgggaaa ccgctcagcg
    11701 caggagttgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac
    11761 cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt ggagaggagg
    11821 gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt catcgcctga gtcaccagtt
    11881 catgttcatg gcgatctttt tctgacgcca gcagacgatt acccgcttcg cgatcgagca
    11941 cttcatctgg ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt
    12001 ctttacgtag cagcagttcc gggctggccc ccagcagaac gccaccatca gccaacggaa
    12061 catggaagtt gtaactaacc gggttttgcg caatcaaccg ttccagcaat acgccactat
    12121 caatggcggc gtcagtggtg atatcaatca accgtgacaa caccactttg tcgacctgcg
    12181 gcgtggcggt aagtgcggca gcacgggcaa ccatctgttc aaacgtggtt tgttccggaa
    12241 tggcctgacg ttccaccacg ttcagcgact ggctgcgggt gaaacggcgt gcggaagctt
    12301 gtttttcctg acgggagaac gactgccagg attcgggaat atacagcgac gaaggctgac
    12361 gtggatcgaa gggaatcgcc ccgaccatca ccggattttt gatgccctgc gctttggcat
    12421 cggcaaacag cgcggcgagt ttttgctgga agggactgtc gggtgaatcc ccgttcacag
    12481 cgggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac ggcgacataa
    12541 agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca gccagtgacg
    12601 tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca tttatatttt
    12661 cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac gcaatctgca
    12721 actcggggtt gcatctggtt gtggttacaa tgaaaatgag aagcattaac ttcattaata
    12781 acaggaagct gatttgtgag actcgcctcg ctctaccgca acgcccttct attaacagga
    12841 cttttgcttt caggaatagc cgcagttcag gccgccgact ggccgcgtca gattactgac
    12901 agccgtggca ctcataccct ggaaagccag ccgcagcgta ttgtatccac cagcgtcacc
    12961 ctgactggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacacca
    13021 aataaccgcg tagcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
    13081 cgcaaactgc aacggctcta tatcggcgaa ccgagtgcag aagccgttgc cgcgcaaatg
    13141 ccggatctga ttttaattag cgcaaccggc ggcgattcgg cgctggcgct gtacgatcag
    13201 ctttctacca tcgccccaac attaatcatc aattacgacg acaaaagctg gcagtcgctg
    13261 ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcactg
    13321 tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact
    13381 gcccttgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
    13441 gggcagatgc tggaacaact cggctttacg ctggcgaagt tgcccgcagg cttaaacgcc
    13501 agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggagaaaa tctggctgca
    13561 gggttaaatg gcgagtcact attcctgttc gccggtgatc agaaagacgc cgatgctatt
    13621 tatgctaatc cgctgctcgc gcacctgcct gcagtacaaa acaagcaggt ttatgcgctg
    13681 ggaaccgaga cattccgcct ggattactac agcgccatgc aagtgctgga acgacttaag
    13741 gcgctgtttt aagcattaat tgtcggacgc tgtcacctgc ggcggcgtct ggcgaaaacg
    13801 ccgcaactcc accagcacca gcaacaacaa cacgccgata atcaacaaac caaaaccgct
    13861 cgcgcttgcg gaagcaaccg gtgtcatcat cgctcccaga ccgcctaaca gcgccgcacc
    13921 tatggcatcg cccgtcacgt tttgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc
    13981 ttccggggtt tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cgctcagcca
    14041 gccgaacagt gccagacaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc
    14101 aatggcgagg aacgccccca atgtggagag caacatcaac agacctggcc gtacgctatg
    14161 cgccagtttt ccactggtta acgcgccaat agccgcaccg agcgggattg ccgcatagag
    14221 aaagccaatt tgcgccgccg acatctgcca gttgtcagcc agcgccggat acagtacccg
    14281 caccgcgctc gccatcgtca ataaaccccc cagcaacgca atccctccca ccagcgggct
    14341 ggcgagtaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg
    14401 cggtggtggc aacgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc
    14461 cgccagcccg tagttccagg ctacgcctcc ggtcgccagc agtaaaccac caatcatggg
    14521 cgaaatcacc gaccccagac gcacagtcaa catggtgatc gccccggcct gcattaggtt
    14581 ttcacgtcct accagtgcag gtgtcgccgc cagtagcgcc gtaacgccaa gtgatgcgaa
    14641 aaaaccatcc cataaaccaa gtaaatagat tgccagcaaa gacggctccg gcagcagcgc
    14701 attcaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt
    14761 tttgcgctca tagcgatccg ccagcacacc accgatcatc agaccgacaa acatagcacc
    14821 gccggtcagc gtcaccgaaa gccccacctg ccaggtagaa tgtgtcatca tctggatctg
    14881 caccggcacc gcgacgctga gcaaacccag agacacaatt gagataaaac gagcgaggaa
    14941 tactgcgcga aacgccgggt gcgtttttaa caggctgagg ttaagcagcc aggattgttt
    15001 attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct
    15061 aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt
    15121 ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta ttactgatta
    15181 tcgcgacggc attaagcctg ctcattggag caaaatcact ccccgcttcc gtagtgctgg
    15241 aagccctctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcacgactgc
    15301 cgcgcaccct tgccggttta ctggcaggcg gtgcgcttgg ccttgccggg gcgttaatgc
    15361 aaaccctcac ccgaaaccca cttgccgacc ccggcttgct tggcgtgaat gccggagcca
    15421 gctttgccat tgtgctgggt gcggcgctgt ttggttactc ttccgcgcag gaacaactga
    15481 tgatggcctt cgccggcgcg ctggtggcgt cattgattgt tgcctttacc ggcagccagg
    15541 gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg gcggcggtgc
    15601 tggaaggact gaccagcggc atcgccctgc ttaatcctga cgtctacgat caactgcgtt
    15661 tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaagtg gtgctgatcc
    15721 cggtgcttac cgccggagca accgcgctat tactgagccg cgcgctgaac agtttaagcc
    15781 ttggcagtga caccgcgacg gcgctgggca gtcgcgtagc gcgcacacag ttgattggtc
    15841 tgctggcaat taccgtgctt tgtggtagtg caacggcggt agttggcccg attgccttta
    15901 ttggcctgat gatgccgcac atggcgcgct ggctggtagg tgccgatcat cgctggtcgc
    15961 tgcccgtcac gctactcgct acccctgccc tgctgctgtt tgccgatatc attgggcggg
    16021 tgattgttcc cggcgaactg cgcgtttctg tagtcagtgc ctttattggt gcaccagtgc
    16081 tgatcttcct cgtacgacgt aaaacgcgag gtggcgcatg atttacgtct ctcgccgatt
    16141 aattatcacc tgtctactgt tgctaatagc ttgtgtaatg gcaggcgtgt ggggattacg
    16201 cagcggtgcc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc
    16261 gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt
    16321 gattggcgcg gcgctgggcg tcagcggcgc gatttttcag tcgctgatgc gtaacccgct
    16381 cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat
    16441 ggtgctgttt ggtcaggatc tgacggctat cgcgctggca gcaatggcgg ggggtattgt
    16501 cacctcgctg ctggtctggc tgctcgcctg gcgtaacggt atcgacacct ttcggttgat
    16561 tattatcggt atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa
    16621 agcgtcttta gaaacggcgc taacagcggg tttgtggaat gccggatcgc tcaatggcct
    16681 gacgtgggca aaaacctcac cttccgcacc catcattata ttaatgttaa ttgccgccgc
    16741 cttgctggtg cggcgaatgc gcttgctgga aatgggcgat gataccgcgt gtgcgctggg
    16801 cgtcagcgtc gaacgttcgc ggctgttaat gatgctggtc gccgtagtgc ttaccgcagc
    16861 tgccactgcc ctggcggggc caatttcctt tattgcttta gtcgcgccac acattgcccg
    16921 ccgcatcagt ggcaccgctc gctgggggct gacccaggcg gcgctgtgcg gggcactttt
    16981 actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac ttccggttgg
    17041 cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc ttgttaattc aggagtctcg
    17101 caaaaaatga ccgaatcagt agcccgtttg cgtggcgaac agttaaccct cggatatggc
    17161 aaatataccg ttgcggaaaa tctgactgta gaaatacctg atggtcactt cacggcaatt
    17221 atcgggccaa atggctgcgg taaatccacg ttactgcgta ccttaagccg cctgatgacg
    17281 cctgctcacg ggcatgtctg gctggatggc gagcacattc aacattacgc cagtaaagag
    17341 gttgcacgcc ggattggtct gttggcgcaa aatgctacca caccgggcga tatcaccgtg
    17401 caggagctgg tggcgcgtgg gcgttacccg catcaaccgc tgtttacccg ctggcgcaaa
    17461 gaagatgaag acgcggtaac aaaagcgatg caggccacgg gaataactca tctggcggat
    17521 caaagcgtgg acaccctttc tggcggacaa cgccagcgag cgtggatcgc gatggtgctg
    17581 gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga tatcagtcat
    17641 cagattgatc tactggaact gttaagcgaa ctgaaccgcg agaaaggcta taccctggcg
    17701 gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat tgcattgcgg
    17761 gaagggaaaa ttgttgctca gggagcgccg aaagagattg tcactgctga actgatcgag
    17821 cgcatttatg gtctgcgctg catgataatt gacgatccgg ttgccggaac gccgctcgtg
    17881 gtgccgctcg gacgaacggc accttcaacc gcaaagattt aaactaagtg gtctgccatc
    17941 atggcatcct gttttctgga tgccatcgca tgacgcaaca acacaccacc acaagccacc
    18001 atcccgccga ccaacgcaga aaggatcacg ataatcgcct taccctgacc gtcttttttt
    18061 acgggtaatg acggacgcaa ctgatattta aacggtgtaa aattcacatc gttgacattt
    18121 gtttttgtta attgctcgac tagatactga cgattacgta attcaccgtt caattccgca
    18181 acgtcagtga ctgctttttc aatttccagt ttgcgttcaa taccgtctgc accgagagaa
    18241 atagagaaat cggggtcatc tttaacggcc tgaccattac tgtatacggg ctttttaatt
    18301 cctgccgcgt tggcaatgtc aagtgaataa ttgaggcgct gaatgtttgc atcaagttga
    18361 tttttcgttt taatgcgatc ctgagccagt ttttcttttt caaactgggt tttgatctcc
    18421 agtttattac ggacgttttc tatcgactct ttcaccacca acgcagagat ataatcgata
    18481 tacccggcca acactttctg tgcctcttca ctggtgggtg cggtgaaact tagcgtccag
    18541 gaagtataca atgcagactc atcttttttc ttactggcat tgtcatcaac cgctttcatt
    18601 ttttcgctca atgcaacaat ggcgcgatgc agatccagtg ggtcgatttt cgcttctttt
    18661 aattgatcca tcacataagg tgatgaacgc aggtactcct caagcaagct aacagactga
    18721 aatttcttga taaacaggtt aaatgcttct gtacgatcaa ttttgatatc cagatccagc
    18781 acacgaagct tagtgaatgt tttctccagc tcttgccact gaacaggttc tggaggcgtg
    18841 acaaccgccg cgctagtcca tttttgcggc aggatgaaag agatcagcaa gcctgcgcag
    18901 gcaaacgcaa aaacgaccgc catgaccgtt ttttttgccc gccataaaac ctcgattaga
    18961 ttaagtaaat caatttcatt attactgggc gacgccagag gatactcggg aaaatgagct
    19021 tcacttccct gtttaatatt cagtgatgac atgtagtgaa acctgaggtt ttagtccgta
    19081 aagcacaacg atgtacatag cattctaaca ggaactgaga tttatccgaa agcactcttt
    19141 cactattatt attctcaaaa tgaataacaa acttagcaac cccagcaatg acgctgctct
    19201 cagaaaaata aataaatttt aagaataaaa aaaccataat catttagtcg gattatggtt
    19261 ttaaatgagg ttatggcaag ttctttacta acgtacggtt agttattaat ttgtgtattt
    19321 attagtggtc ctatttcttt aaaatactcc ggggaaataa tatccacatg cgcacaatcc
    19381 tgacgataga tatccagctc agcaatccac ggtgcccagg cctgttcagg actcatacct
    19441 tcctgaagcg tgcgttcggc aacaaatagc gtcgctttac catcaaacgc tacgctatga
    19501 gctgtcgtca gcaggcgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc
    19561 gttgaagtac ttccctgctg tgccgccagg aaggcttcgc gctcgcggtt aatctccgcc
    19621 agcacttccg ggtccagacc attggcttct ttttcctgcc agttttgcgt ttctggcggc
    19681 caggtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca
    19741 atgccctgcg ccagcgtacc gccaagtgaa taacccagca ggtaataagg gccgtgcggt
    19801 tgttgttcaa ataacgttgc cagatgcgct tcgcagactt catccaggtt tgccgccgtc
    19861 tgcatggggc catgagggcg cggcgactga atgccgataa tcgaccattg tggatcgaga
    19921 taacgcgaga gcacgctgaa ctgccaggca aaaccggatg ccggatggaa acaaaacagc
    19981 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg tcgggtgctg
    20041 tcttcttcac catcaataat cgttgccagt ttggcgacag ttgacgcgac catcacctgc
    20101 cccggcgtca cctgacgggc aaactgtcga cttaactgcg ctgccagttt cattgccagt
    20161 agcgaatgac cgccaagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc
    20221 aacgacgaga atgccgcggc gataatcgtt tcactgcccg ctttcggcac acgccccggc
    20281 gtttgtgcct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
    20341 agtggtaact gtggaagttg cagcagaacc accggtacca tatgtggtgg caatgtttct
    20401 cgaagctgcg cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag
    20461 cccaccaact gacgcgcatc accaccagtg gctgccgcct ggttaatcac acaggcgtgg
    20521 gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc gatcaatttc gcccagttcg
    20581 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc cactgcgccg
    20641 ttatccagcc agcgggcaac gtctccggta cggtacatcc gttcacctgg ggcaaaagga
    20701 tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttga
    20761 ataccggtga gatagagatc gcccgccaca cccggcggca ccggatgcat catcgcatcg
    20821 agaatgcgca ggcccgtatt ccacaccgga taaccaatcg gcacactgct gccgcgcacc
    20881 tctgccagtt cctcgccaaa agccggatac caactgacat ctaccgccgc ttccgtcggg
    20941 ccatatagat tatgcaacgg cgcgccggtt aactgttgcc attcgcggca taaatcagcc
    21001 ggtagggctt caccactaca gaaaacctgt ttcaacgtcg agcaattctg acgagcggtt
    21061 tgcggcgtca gcgatgcaac aaatgctgcc agcatcgacg gcacaaagtg cgtggtcgtt
    21121 acaccatatt cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca
    21181 gccatcacca gtttagcccc ggcaataaac ggccagaaaa actcccacac cgagacatca
    21241 aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcgggta gtggttttgc
    21301 atccaaagca ggcggttaac gatagccgtc tgcccgacca ttaccccttt cggcctgccg
    21361 gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag ctgcagcggc
    21421 gcactgccct gcggtgtaag cggggcgtta tagcaaaggc gggttaaatc tggaacatcg
    21481 gcaaagcgcg gcagttgatc gtcagtggtg attaacagcg acggacgcgc atcttccagc
    21541 atcattttca ggcgatcgtc cggatagccg gtatccagcg gtaaccaggc cgcaccagcc
    21601 tcaacaattg catgtagcgc gagagtcaaa aagaccgagc gcggtagagc caccgccacg
    21661 ctgtcgcccg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctcg
    21721 cgcatttccc gatagctgaa ctggtaacgc gcatctgcca gcgccggagc atccggtgtt
    21781 tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc aatctgagtg
    21841 gcgttgatct gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac
    21901 aacagcgccg gatccgcggc gaactgggca atcagcattt tcaggcgttc agcgtgttga
    21961 attaacgttg gctcatcgta acgctgttta ttggcgagga tttcaatact caaatctccg
    22021 tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc caggggatgg
    22081 gtttgcgcct gaacaccagg aatatccagt tggtaatcaa ataccttgat attgagtacc
    22141 ggaccgaaca gcggttcctc tcctgccgct cgcccactgt cacggacaat ttgttcggcg
    22201 tcgtagcgtt gatggcggcg catttttttc agttgtgctg ccagtcgggt tgccagttgc
    22261 ggcagcgttt cttgcgccgc aatgtgaata cccaacggta aaacgttgag cacgggtccg
    22321 gtagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc agcgtagtcc
    22381 atgcggttgc acaatcgccc cagccacaag gctgccaacg caagtgctaa atcggtacgc
    22441 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa ttccagtttc
    22501 aggcgcaaaa tatctgccga agcgctgcgc cccggtaaag gtgccggaga aagtgacgcg
    22561 ggcggtggca gttgacgacg ctgttccgcc cagaatgccg catcacgctg ccaggcttca
    22621 ctttcgcggt actgctgata ctcttccact acatcagcga aaggcgtaaa cggcgaagca
    22681 ggcgttggtt cgccacgcag caatgcgcag taaatattgg cgatctggcg tgtaatggcc
    22741 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
    22801 tccgccacct gtatcagctg atgaaagacc agtggtttac cactatcgac gcgcagatct
    22861 tgttgcaaat ccgcctgcat taatgcccgc gcagtaccgt gcggatcaat gttggttcgc
    22921 aggtcgataa tttctggcag ttcgaacgtc tgcgcatcat cgacccactg ccagacttcg
    22981 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc ggcaaccacc
    23041 gcgcgggcca gtagtggcgc atcaacctct ccggttaact caacgtaatg cgccacgctc
    23101 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
    23161 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattcgcc ggtgtcagag
    23221 tacgccagtg ggcttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
    23281 cgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
    23341 tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca
    23401 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
    23461 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttagggg
    23521 gtgtaattgc gcatacagcg cctgattggc ctgcatgatc atcggctcgc gaacacccgc
    23581 ttccagcaca atgcgcagac cttcggcgct aacttcacca gtttttagct gttcaagtaa
    23641 catgccctct tggtgcccgc cccgatgcgg ccaccagtac gaacctgact ggcttaatac
    23701 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagcc caccaaaact
    23761 ctgtccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcacctg
    23821 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgggc
    23881 gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg gcgggcggaa gttgccgacg
    23941 atgggtcagc gaagtcagcg caggccagac gggcatactc tgcgcccaaa attcgccatc
    24001 aagcaaaact gccagcgggc gttcttctgc tgttgcatcg ccgatggtaa aaatccatac
    24061 acggcgtgaa tttttcaacc gttcactttt ccagataatt tctttggcag gcgtttctgg
    24121 cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac
    24181 agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta tcgcctgggg
    24241 taatagtttt cgccagcctt cgcgtaattc gaggcgatcg ggggatggtg cagaaaaaat
    24301 gtcatcgcgt tcggtaggaa taaagcagta gctgccgcgc cagttggcat tgagttgtgt
    24361 cgtccactgc caaacatcgg ttcctgcaat tcgctgcatc gaccggggct ggctgttctg
    24421 atggtgatcg gtcatgccag tgatatagac ccatacgcgc tttatcgccg agtggccttc
    24481 agaaccttgt ggatcacgcc accaaaaagt gacttcaaac atttcgtcat tcagacgttg
    24541 ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac
    24601 accataaccc catgtttact
//
LOCUS       DAJOTB010000067        24189 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJOTB010000067 DAJOTB010000000
VERSION     DAJOTB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.067"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            45..596
                     /locus_tag="OHN28_002593"
     CDS             45..596
                     /locus_tag="OHN28_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCQ2297972.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(767..1759)
                     /gene="rpoS"
                     /locus_tag="OHN28_002594"
     CDS             complement(767..1759)
                     /gene="rpoS"
                     /locus_tag="OHN28_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCQ2297973.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1822..2961)
                     /gene="nlpD"
                     /locus_tag="OHN28_002595"
     CDS             complement(1822..2961)
                     /gene="nlpD"
                     /locus_tag="OHN28_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCQ2297974.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3101..3727)
                     /gene="pcm"
                     /locus_tag="OHN28_002596"
     CDS             complement(3101..3727)
                     /gene="pcm"
                     /locus_tag="OHN28_002596"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCQ2297975.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3721..4482)
                     /gene="surE"
                     /locus_tag="OHN28_002597"
     CDS             complement(3721..4482)
                     /gene="surE"
                     /locus_tag="OHN28_002597"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCQ2297976.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4463..5512)
                     /gene="truD"
                     /locus_tag="OHN28_002598"
     CDS             complement(4463..5512)
                     /gene="truD"
                     /locus_tag="OHN28_002598"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCQ2297977.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5509..5988)
                     /gene="ispF"
                     /locus_tag="OHN28_002599"
     CDS             complement(5509..5988)
                     /gene="ispF"
                     /locus_tag="OHN28_002599"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCQ2297978.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5988..6698)
                     /gene="ispD"
                     /locus_tag="OHN28_002600"
     CDS             complement(5988..6698)
                     /gene="ispD"
                     /locus_tag="OHN28_002600"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCQ2297979.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6717..7028)
                     /gene="ftsB"
                     /locus_tag="OHN28_002601"
     CDS             complement(6717..7028)
                     /gene="ftsB"
                     /locus_tag="OHN28_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCQ2297980.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7221..7544)
                     /locus_tag="OHN28_002602"
     CDS             complement(7221..7544)
                     /locus_tag="OHN28_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCQ2297981.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7593..8198)
                     /gene="cysC"
                     /locus_tag="OHN28_002603"
     CDS             complement(7593..8198)
                     /gene="cysC"
                     /locus_tag="OHN28_002603"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCQ2297982.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8198..9625)
                     /gene="cysN"
                     /locus_tag="OHN28_002604"
     CDS             complement(8198..9625)
                     /gene="cysN"
                     /locus_tag="OHN28_002604"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCQ2297983.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9627..10535)
                     /gene="cysD"
                     /locus_tag="OHN28_002605"
     CDS             complement(9627..10535)
                     /gene="cysD"
                     /locus_tag="OHN28_002605"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCQ2297984.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGSLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10787..11824
                     /gene="iap"
                     /locus_tag="OHN28_002606"
     CDS             10787..11824
                     /gene="iap"
                     /locus_tag="OHN28_002606"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCQ2297985.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11950..12102)
                     /locus_tag="OHN28_002607"
     CDS             complement(11950..12102)
                     /locus_tag="OHN28_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2297986.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12368..13102)
                     /gene="cysH"
                     /locus_tag="OHN28_002608"
     CDS             complement(12368..13102)
                     /gene="cysH"
                     /locus_tag="OHN28_002608"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2297987.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13176..14888)
                     /gene="cysI"
                     /locus_tag="OHN28_002609"
     CDS             complement(13176..14888)
                     /gene="cysI"
                     /locus_tag="OHN28_002609"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCQ2297988.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14888..16687)
                     /gene="cysJ"
                     /locus_tag="OHN28_002610"
     CDS             complement(14888..16687)
                     /gene="cysJ"
                     /locus_tag="OHN28_002610"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCQ2297989.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            17006..17368
                     /gene="queD"
                     /locus_tag="OHN28_002611"
     CDS             17006..17368
                     /gene="queD"
                     /locus_tag="OHN28_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCQ2297990.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            17446..18717
                     /locus_tag="OHN28_002612"
     CDS             17446..18717
                     /locus_tag="OHN28_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2297991.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18708..18968
                     /locus_tag="OHN28_002613"
     CDS             18708..18968
                     /locus_tag="OHN28_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2297992.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18985..19560
                     /locus_tag="OHN28_002614"
     CDS             18985..19560
                     /locus_tag="OHN28_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCQ2297993.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19708..20568)
                     /locus_tag="OHN28_002615"
     CDS             complement(19708..20568)
                     /locus_tag="OHN28_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2297994.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20565..21344)
                     /locus_tag="OHN28_002616"
     CDS             complement(20565..21344)
                     /locus_tag="OHN28_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCQ2297995.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(21322..22731)
                     /locus_tag="OHN28_002617"
     CDS             complement(21322..22731)
                     /locus_tag="OHN28_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2297996.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22753..>24189)
                     /locus_tag="OHN28_002618"
     CDS             complement(22753..>24189)
                     /locus_tag="OHN28_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2297997.1"
                     /translation="AIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLPI
                     PAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQI
                     INIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFST
                     LYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKIF
                     KFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCV
                     LIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQI
                     LKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMY
                     FVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGS
                     AWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         5662 a         6631 c         6084 g         5812 t
ORIGIN      
        1 aaacctgcct caaaaaatca ttcattagta aaaaggatat ttatatgcaa aacatagctg
       61 tggtttatta ttcaggatat ggtcacacta aactggttgc tgaaaaagtg gcacaaggcg
      121 cccatgcaga tttaatttcg attgataacg aaggaaatat cactgagcag gagtgggaaa
      181 aactggacgc agccgatgcc attatcttcg gtgctcccac ctatatgggc ggtgcgccat
      241 ggcaattcaa gaaatttgct gatgccagtt ccaaagcgtg gtttacacgt aaatggcagg
      301 ataaagtctt cgggggtttc accaacagcg ccagcctgaa tggcgataaa caagttacgc
      361 tgatctatct gcaaacgctg gcttcacagc atggcggtct gtgggtgagt ttaggtcagg
      421 ctcctgccaa tgtgcttgcg tcaaccaggg aagatgtaaa caatctgggc ggttcggtcg
      481 gtttgctggt gcaatctcca gccgatgcgg gtgcagagca aatccctacg ggcgatctgg
      541 atacagccgt gaaatatggt gaacgcgtgg cgacaattac tgcgcgctta aaatgatgat
      601 tgccgaatgt gatgctgacg cgtctatccg gtcttctaca tctaaaaagt gccgtcccct
      661 ggtcagtcag ataaagtttt aacgctgtat ccgataacag ttcaacatgt accaaaagaa
      721 aaaaagccag cctcgcttga gactggcctt tctgacagat gcttacttat tcgcggaaca
      781 gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg caaacggcgc aggccttcaa
      841 cctgaatctg gcgaacacgt tcacgggtga ggccaatttc acgacctaca tcttccagtg
      901 ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag cacttcacgc tgtttggcgt
      961 tcagctcgaa cagccatttg acgatgctct gcttcatatc gtcatcttgc gtggtatctt
     1021 ccggaccatt ctctttttca tcggccagga tgtccagcaa tgctttttcg gaatcaccac
     1081 ccagcggggt gtctaccgag gtaatgcgct cgttaagacg aagcatacgg ctgacgtcat
     1141 caactggctt atccagttgc tctgcgatct cttccgcact tggttcgtgg tccagcttat
     1201 gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc ctttacgatg tgaatcggca
     1261 aacgaatagt acgggtttgg ttcataatcg cccgttcgat cgtctggcga atccaccagg
     1321 ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa cttctctacc gcgcggatca
     1381 gccccaggtt gccctcttcg atcaggtcca gcaacgccag accacgattg ccataacggc
     1441 gggcaatttt taccaccaga cgcaagttac tctcgatcat ccggcggcga gaggcgacat
     1501 ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc ggccgttaac agtggtgaat
     1561 aaccaatctc accaaggtaa agctgagtcg cgtccagcac acgctgtgtg gctccctgcg
     1621 ataacagttc ctcttcggcc aaatcgttat cactgggttc ctcttctact aaggcctttt
     1681 cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc ttcatttaaa tcatgaactt
     1741 tcagcgtatt ctgactcata aggtggctcc tacccgtgat cccttgacgg aacattcaag
     1801 caaaagcctg gttccgccga tttatcgctg cggcaaataa cgcagcgggt ttacggattt
     1861 ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg gttccggtgc tacccatggt
     1921 tgctattttt tgccccgcct tcacttcttg ttgttcccgg accagcattg tgtcgttatg
     1981 ggcgtaggca ctcaggtaat catcattatg tttgatgata atcagattac cgtagccgcg
     2041 cagcgcgtta ccggcataaa caacgcggcc atctgcggtc gcgataattg cctgtccttt
     2101 gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa gcgccaaagg tttcgatcac
     2161 tttgccctca gtcggccagc gccaggtgga gataggcgta ctggttgatg tactgctgac
     2221 agtcggctcg gttgtgcttg ctgttggtac cgttacaggc actgtgaccg tggtcgcagt
     2281 tggcttgttg ttcggcaaca ttttgttagc actctgttca cccgaagact cagaatacgt
     2341 aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt gcaggcttga tcacaactcc
     2401 ttgctctgct gcgtcggcct gggtaatggc atttccgcct gtgattggcg taccggaagc
     2461 attacccacc tgtaaggtct gaccgacgtt cagcgcgtat ggtgcctgaa tattgttgcg
     2521 ctgagcaagg tcacggaaat cgttgccagt aatccaggcg atatagaaaa gcgtgtcgcc
     2581 ttttttcacg gtgtatgtac tgccgctata actgcctttc ggaatgttcc catactgacg
     2641 gttgtagacg attcgtccgt tttccatctg taccggctgc tgagctactg gctgcacagg
     2701 ctggatttgc ggttgttgag tagcctgaat ttgtggctgc tgtaccggct gaatttgcgg
     2761 ttgctgcgct gtagacgtcg tccccatttt cggcggcggc gtaatcaaca taccagaatt
     2821 ggtatttgcg ggcgcattgc cattaacgga gctgaccggt gccggtggat ttgaagtgtc
     2881 agaacagcct gccagccata gcgaaaccag tgacaatgcc gcaatgcggc gaacggtgaa
     2941 ttttgggctt cccgcgctca tttatccccc aggaaaaatt ggttaataac cagtgacata
     3001 attaccgtgc aaggcaccat actgaacact ggaaaagatg ttcacgatac gctgacttgc
     3061 ggcaaaataa ccaggaaaaa tccaggtatt tcctcacgtt ttaagccagc tcacccttca
     3121 ctaaagggac aaagcgcacg gcctccacgg tatcgataat aaattcgcct ccccgacgac
     3181 gcacccgttt caaatactgg tgctcctccc ctacgggtaa gacgagaatc ccgccttcgt
     3241 ccagctgcgt cattagcgca gttggaattt caggcggtgc cgccgtaaca atgatagcgt
     3301 caaacggcgc gcgtgcctgc caaccttgcc atccatcgcc atgacgggtt gaaacattat
     3361 gtaaatcaag atttttcagg cggcgacgcg cctgccactg caagccttta atccgttcaa
     3421 ccgagcaaac atgctggaca agatgcgcca ggattgcagt ttgatatccc gaaccagtgc
     3481 caatttccag cacccgcgac tgcggcgtta gctcgagtaa ttcggtcatt cgcgccacca
     3541 tatacggctg cgaaattgtc tgcccctgac ctatcggcaa ggcgatattg tcccaggctt
     3601 tttgttcaaa cgcttcatca acgaattttt cacgcggcac ggcggcaagt gcattcagca
     3661 cctgctcatc ctgaatacct tgcgcacgta attgatccag aagtgcttgt acgcgtctgc
     3721 ttaccattgc gtgccaactc ccacgctgtt taaccagtct gaaaccacat cttgcgcgct
     3781 atgcgcagtt aaatccacat gcagcggcgt gatggaaaca tagccctcat ctaccgcagc
     3841 aaaatcggtc cccggaccgg catcacattt accgcccggc gggccaatcc agtacagcgt
     3901 attgccgcgc ggatcttgct gcgggatcac ctgatctgcc ggatgtcgtg taccgcagcg
     3961 cgtcacgcga atacctttga tttgatccaa tggtaaatcc ggaacgttaa tattaagaat
     4021 acgcccggtg cgcagcggct ctttacacaa tgcgcgcaaa attgagcagg ttaccgccgc
     4081 ggcagtgtcg taatgtttat gcccgtcaag cgagacggca agcgccggaa aacctaaatg
     4141 acggccttcc atcgcggccg ctaccgtacc ggaataaata acatcatccc ccagattcgg
     4201 cccggcgtta attccggaca caacaatgtc cgggcgcggg cgcatcagag catttacgcc
     4261 aagatagacg caatcggtcg gggttcccat ttgcacagca atatcaccat tttcaaaggt
     4321 aaacgtgcgc agggaagatt ccagtgtcag agaattagaa gcgccgctgc ggttacgatc
     4381 gggggcgacc acctgaacgt cagcaaactc acgcaaggct ttcgccagcg tttgtatacc
     4441 gggtgcatgt accccgtcat cattactcag caatatgcgc ataatcacct gttgtgttga
     4501 taagttccct gacaacgctg gttgcaaaac ttcccgccgg aagccagaaa cggatttcta
     4561 cggtgacgtc atcccaccag ttccagctta attgttgcgg atacagcagc atcgctctgc
     4621 gcgcggcttc aactttttca cgcaccagta aagtttgtaa ttcagtttct tcggcgacag
     4681 ctgcttgttc gaatgccagc gcttcacgct gagttcccca ttcgccactg cccagcaatg
     4741 cggcggttat cattagctct ttatcgttga cgcgacgctg taattccgcc agttcttcgg
     4801 tggttgcaac aaaccagcta ccacgtccgg ctaattgtag cgcatcgccg tcaacaactt
     4861 gattaacgtc tgcttttttg aggcgctcag caacaatctg attaaacaac gcactgcggg
     4921 ctgccgacaa ccaaaaactc cgtttattgc gatcgcgcac cggagtattg gtttgcgccc
     4981 agcgcagcgc gccctgcaag ttgctaccgc caatcccaaa acgttgggca ccgaagtagt
     5041 tcggtacacc ttttacgcaa atatcgatca gacgttgttc aacgtcatcg cgattgctca
     5101 cttcgcgcag aaccagggta aaggcgttac ctttcagcgc gcctaaacgc agcttgcgct
     5161 tgtgccgcgc atactccagc acctggcagc cttccagttg aaaggcgctc agatcgggca
     5221 tctctttgcc cggcacgcga gcgcataacc actgttccgt aacagcatgt ttatcttttt
     5281 gcccagcgaa gctgacttca cgggcatgaa ttttcaggaa tttcgccagt gcatccgcca
     5341 caaaacgggt attgcagcca tttttgagga ttctaaccag aatatgctca ccttcaccat
     5401 caggctcaaa gcccaaatct tccaccacca caaagtcttc cggattggct ttcagcagcc
     5461 cggtgccttg cggtttaccg tggaggtaag tgagattatc aaactcaatc attttgttgc
     5521 cttaatgagt agcgccaccg cttcacaggc aatcccttcc ccacgtccgg taaatcccag
     5581 tttttccgta gtagtggctt tcacgttaac atcatccata tggcagccga gatcttcggc
     5641 aataaacacg cgcatttgtg gaatgtgcgg caacatcttc ggtgcctgag cgatgatagt
     5701 gacatcgacg ttgccgaggg cataaccttt cgcctgaata cgacgccagg cttcgcgcaa
     5761 cagctcgcgg ctgtcggcac ctttaaatgc cggatcggta tccgggaaca gcttgcctat
     5821 atctcccagc gccgccgcgc caagcaatgc atcggtcaac gcatgcagcg ccacgtcgcc
     5881 atcagaatgc gccagcagcc ctttttcgta aggaatgcgt acgccaccaa tgataattgg
     5941 gccttcaccg ccaaaggcgt gtacgtcaaa accgtgtcca attcgcatta tgtattctcc
     6001 tgatggatgg ttcgggtgag gtaaaattcg gccagcgcca aatcctccgg gcgcgttacc
     6061 ttgatgttat ccgcgcggcc ttcaacaagt tgcggatgaa aaccgcaata ttccagcgcc
     6121 gaggcctcat cggtaatggt agcgccttca tttagagcgc gcgtcaggca gtcatgtaac
     6181 agctcacgag ggaaaaattg cggcgtcagc gcgtgccata agccgttgcg atcaacggta
     6241 tgagcaatgg catttttgcc cggttcggca cgtttcatcg tatcgcgcac cggcgcagcg
     6301 aggatccctc ccgtgcggct ggtttcgctc aacgccaaca atcgcgcgag gtcatcctga
     6361 tgcagacaag gacgagcggc gtcatgcacc aatacccact gcgcgtcgcc agcggctttc
     6421 agacctgcca gcacggaatc ggcacgctct tcaccaccat caacaaccgt gattcgtgga
     6481 tgattcgcca gaggaagttg tgcaaagcgg ctatcgccag gacttatggc aatgacgaca
     6541 cgtttcaccc ggggatgcgc cagcagcgca tgcaccgagt gttcaagaat ggtttgatta
     6601 ccgattgaga gatattgctt aggacattcc gtttgcattc ggcggccaaa tccggccgcc
     6661 ggaaccacgg cgcaaacatc caaatgagtg gttgccatgt taattcccgg gctgatttat
     6721 cgattgtttt gccccgcaga ctgtgcgcgc tttgacgcgt caggcaccag acgataaaac
     6781 gtttcgcccg gcctggtcat gctgagttca ttacgcgcac gctcttcgag cgcctcctgg
     6841 ccgccattga gatcgtcaat ttcggcaaaa agctgatcgt ttcgcgcttt aagtttcgcg
     6901 tttgtagctt gctgtgccgc cacatcatta ttgacgcgag tatagtcatg tataccgttc
     6961 ttaccgaacc acagcgaata ctgtagccag accagaatag ccagcaacag cagcgttagt
     7021 ttacccatcc tgccccctga aaaacggcat cttcatccca tgcatccgaa gacgactcta
     7081 catcctctgt tggggatacc gcgacaacgc gggcaaatgt accacatttg tccattgtta
     7141 cgtataccca gggcgtgcag aaaataatct cattattagt tacggattga attatgaaca
     7201 gaatagacgg gaaagaacaa ttaacccaat agccacataa acagtgcacc aaacataatg
     7261 ccaaccgtcg ccagagtgaa aacaatactg tagcgtagct ttccgtccat caatgaatgc
     7321 agtgcaatcc ccaccactac agcgacgggc atcagcgcca gaaagaaagg ccaggtgtag
     7381 ataaaaaaga gcagcgtgtt agagccataa atcaacatcg gaattgccaa cgcgcacagc
     7441 cacgataaaa aaccaaccac agcgcctggc agcgaccatg tggtgtcttc aacctcagta
     7501 aggctgtcgt tatttgttaa tgtaatgttg tggctgttac gcatatctga tcctgttact
     7561 ttgacgaacc gggcatgaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc
     7621 aacagatcta ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt
     7681 tctgccgatt caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg
     7741 gcacgcgctt tcttatataa accttttgga tcgcgggctt cgcaaatcgc cagcggcgta
     7801 tcgacaaaca cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg
     7861 gcgcggtgtg gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc
     7921 gccacttcac cgacgcgacg gatattctct ttacggtcgg catcgctaaa accgagatcg
     7981 ctgcataatc cgtggcgaac gttgtcgcca tccagcagat acgtactgac gccaagttta
     8041 tgtaacgcct cctccagcgc ccccgcgacg gttgatttac cggaaccaga gaggccggta
     8101 aaccacagca caacaccacg atgaccgtgg tgctgttcgc gttgttgcag agttactggg
     8161 tggctatgcc agacgacgtt ttcgtcatgc agagccatta tttatctccc agtaaatcgc
     8221 gcgcacccca atgcgggaaa tggcggcgaa ccagggcatt caattccagt tcgaatgcac
     8281 tgaattcaga tggcgcagca gttgcctggc taactggttc gtgcaccata ccggcaccta
     8341 cggtcacatt actcaggcga tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat
     8401 aacgatctaa caccagtggc tcgtcaaagg tgagatctac caggccgatg ccgttcagcg
     8461 gcaggttttc aacttcgcgt tgggtaaggt tattgatatc aacctgataa cgaatgccat
     8521 caacacgagc acgcgtcttc ttaccggcaa ttttgatgtc gtaactctgg cccggggaaa
     8581 gcggctgttc cgccatccat accacatcca ccgacgcgct ctgcacagcc ggtaacactt
     8641 cgtctgccgc cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg
     8701 tgatcgcttc tccggcaaag gcttcttcgc gatcgccatc aaaggtcacg atccgcgcga
     8761 cgtttgattc cacaccagag ggcagcactt tgacacgttg cccgacttcc acacgaccgg
     8821 atgccagcgt tccggcgtag ccacgaaaat cgagattcgg acggttaacg tactgcaccg
     8881 ggaagcgcat tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga
     8941 gcagtgtcgg accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt
     9001 ccagtgcgga gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaagg
     9061 tcagataatc ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt
     9121 tgttgatcgc cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac
     9181 gggtttgatc gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg
     9241 atgcgccagt cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa
     9301 atttacgctt ctcggtagag aaatagcggt aagccacgtc aatggtgatg ccctgttcgc
     9361 gctcagcttg caggccgtcc accagcagtg ccagatccag cttttcgccc tgggtgccgt
     9421 gacgcttact gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt
     9481 gtagcaggcg accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac
     9541 gcagcaggct tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga
     9601 tttgttgtgc aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata
     9661 gaccccgcct ggtcgcggtc aattacgcgg ccctgacgtt cactggtggt ggaaaccagc
     9721 atctcttcga tgatctccgg cagtgtttgt gcgtttgact ccaccgcgcc ggtcagcggc
     9781 cagcagccca gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg
     9841 tcgatacggt tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc
     9901 gcagcgagat acagtggaac aatgtcaata ttttccagcc agatgtattg ccagatatcc
     9961 tgctcggtcc agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg
    10021 ttgtagttgt gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca
    10081 cggaaagagt agattcgctc tttagcgcgg gatttctctt cgtcgcggcg cgcaccaccg
    10141 aaggcggcat caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata
    10201 tcggtatgtt tcgcgctgcc atgaacgaat ggattaatcc ccatcgctac gccttccggg
    10261 tttttatgca ccagcagctc gcagccgtag gctttagccg tacgatcgcg gaactcatac
    10321 atctcgcgga atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgaacctgga
    10381 taaaacgcct tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc
    10441 atcaccggat ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc
    10501 agttgccgca ggtgggtaag tcgtttttga tccataaccg ttcctttgca ataccgctat
    10561 tttcttgcca tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat
    10621 tagaatgagt agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt
    10681 aaggcaccgg atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt
    10741 catactatgg cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt
    10801 gcgccaccgt accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc
    10861 gtcacctaaa cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc
    10921 gggacgcatg accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt
    10981 tcagcaaatg ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc
    11041 ccgcgataat cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga
    11101 aggcaaaacg ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag
    11161 cgatgctgac gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc
    11221 cgcaggttta ggtgtcatgc tggaactggc cgagcgcctg aaaaatacgc ctaccgagta
    11281 tggtattcga tttgtggcga ccagcggcga agaggaagga aaattaggcg ctgagaattt
    11341 actcaagcgg atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa
    11401 cttaattgtt ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag
    11461 gaaattaacg cgcgaccggg cgctggcaat tgcccgcagc catggaattg ccgccaccac
    11521 aaatccaggt ttgaataaaa actatccgaa aggcaccggg tgttgtaatg acgcagaaat
    11581 attcgacaaa gccggtattg ctgtactttc ggtggaagcg actaactgga atcttggcaa
    11641 taaggatggt tatcagcaac gagcaaaaac agccgcattc cctgcgggaa atagctggca
    11701 tgacgtaaga ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg
    11761 tcgctgccgt gatgttatgc ggataatgct accgttggtg aaggagctgg cgaaggcatc
    11821 ttaatgggtt tgaaatgatt actcccttgc ctgcaggaaa tcaatgccac cagaaacaga
    11881 gtataaaaaa ggggtgcagg aggccaaacc atcacaattc agtacgcggg gttttacccc
    11941 gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca taccacgttc
    12001 tctgatactc aactcacaca acgagtcgcc taccatcaac gtgaatccca gtactgtaca
    12061 acacagtacg atgattgcca caagggcata ttttgtcagc atgtcttgcc tccctgcaaa
    12121 gaagagacta acatccgaat tgctacgttc gaacgcgagc ctcaggttga tagaaatatc
    12181 gcctggggct tttgtccatc tggaaccttg cgaatgctta acgccagaca gcctcaagca
    12241 cccaatgcca ttctatacct gttaattttc cctgcgatgt tcacctttaa atgccggaat
    12301 gctgctcgca aaaaataaaa acgcctccat catttctgac agaggcgttt aatttatccg
    12361 gcaatattta cccttcgtgt aatccacatt ccctttttaa gccaaagaaa cgcgtttctt
    12421 cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc gataaatatc
    12481 cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga taaatagttc
    12541 ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg
    12601 gtaaattggc gcggctgccg gattgttcac ggcgcaggcc agcaaaccag gtttgcgcat
    12661 tcagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa
    12721 tgccttcaac gccctgctcc cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt
    12781 cggtagcacg atacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc
    12841 ggtaggtttc cgggaacaga tagccggtat cggtgaggat cactggaata tccgggcgaa
    12901 tctgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat
    12961 attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc agtttttcca
    13021 gttgggcgtt agtttccgcc agcgccagaa tccggtctac ctttggcagt tcgttcaggg
    13081 cgtttagatc gagtttggac ataagttcct cactgtttgc cttgcctgat gcaacgctca
    13141 cacgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
    13201 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
    13261 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
    13321 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
    13381 aggttgtagc gacccggcgc tttccccacc agacccactt ccgccagcat cgcgcgacca
    13441 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
    13501 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc ttccgccatc
    13561 gccagcgggc aagtcgggaa tgacacgcag gccattgagt tttcacgctg cggcgtgacg
    13621 gcattcatta acccgctttc tttggcgatc ttctcgatct tcgctttctc gctttccggc
    13681 acgccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt gtggatcttc
    13741 gcgatctcca gcaagccggt tttcagcggg cgccccggat aatcaagaat tcgaccattt
    13801 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatcg
    13861 ccgcgtccgg taaactcata cgggcgaatc ggctcgaatt tgatccccgc gcgacgttca
    13921 acttccgctt taaacgtctc aacccctacg cgctccagcg tgtatttggt tttggcattt
    13981 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc cgccaccgcc
    14041 agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta ggttttcttg
    14101 ttgccgtgtt cgatggaaag cccaccgccc accagcaggt taaagcccac cagcttgccg
    14161 ttttcggcga tcgccacgaa gttcatgtcg ttggcgtgca gatcgatatc gttctgcggc
    14221 gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag gatcggctct
    14281 tcatcagtag tggcgacttt ttcctgatcg agccagatct ccgcatacgc gcgggtacga
    14341 ggcagcagat gttcagaaat tttcttcgcc cattcatatg cttccgcatg cagctgcgac
    14401 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttggcggt cgccagcgca
    14461 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
    14521 tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
    14581 gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg cagacgacag
    14641 cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc gcgaatgtcg
    14701 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc gcccttaaag
    14761 ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
    14821 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
    14881 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
    14941 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
    15001 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
    15061 tccagcgcca cagctctgcg ccctgttcgc gcagtttgtc ttgtacgtaa attttttctt
    15121 tttgatcgcg cgaccaggcg agatcgatgc gcgtcagcac gccttctttg acgtaacgct
    15181 gccattccac ctggtagagg aaatcttccg taaagtgtgg gttgccaaag aatagccagt
    15241 ttttacccgg cgcttcgtcg gcggcacgct gctgcataaa ggcacggaac ggcgcgatgc
    15301 cggtgccagg gccaatcatg atcaccgggg tttccggatt ggttggcagg cggaagttgt
    15361 cgttatgttc gataaatacg cggacttcac cctcttcttc cacacgatcc gcgaggaagc
    15421 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtaa
    15481 cgtgcacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg cgcggggtca
    15541 gtgggcgcag cagattaatc agcgcttcgg catccagttg cgccggagag aaacgcacca
    15601 tgtcgacaat cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
    15661 gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg gcggtgttga
    15721 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
    15781 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccggat
    15841 cgttttgata ccagacgccc agcgcgtcac ccggctggta acgcaggtca gagtcaccta
    15901 agtcaatttc aatgtgacga acgtcttttt cagagttacg cccggtaatt ttctggttaa
    15961 cagagaggct cgccaccagc ggcgcgtctt tgctatacgg gctggtgtgg atttcattta
    16021 ccgtgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
    16081 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg gcatcgacac
    16141 ggtcgagcag gcgttcaccg cccagttccg ccagcttact gtcaaaatct ttcccggact
    16201 ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca
    16261 gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
    16321 ccccttcccc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg atttgtttga
    16381 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgctaat aaatcatcgc
    16441 gtaatgcttc agcaacccga cgcgcattgc cagtttgcga ggcggagata atagttatac
    16501 ccggcatttc tgcggcgggc gctggcgtcg ctgcaagcgc agcaggctgc tggttgagca
    16561 cgccccagaa atagccagaa acccaggcaa gctgggtagg agttaaatcg gtcgtggccg
    16621 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
    16681 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
    16741 aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
    16801 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
    16861 taaccaaccc aatgaaaaac aaatgaatat tgcccatcaa taagataaat cagcgatttt
    16921 gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt
    16981 tttatcgtca tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
    17041 agccgctcac cgcttgccgc acgtcccaca agggcataaa tgtggtcgcc tgcacgggca
    17101 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
    17161 cgatttcgct gaactgaaag cggcgtttaa accaacttat gagcgcctcg atcaccatta
    17221 tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa aatggatttg
    17281 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
    17341 cgcaggttgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag
    17401 taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga agacgactgc
    17461 gacattatta ttatcggtgc cggaattgct ggcaccgctt gcgcgttacg ctgcgcgcga
    17521 gcgggtttat ccgttttgtt actggaacga gcggagattc ccggcagcaa aaatctttcc
    17581 ggcgggcgtt tatataccca tgcgctcgcg gaactcctcc cgcaatttca cctgaccgcg
    17641 ccccttgaac gacgcatcac tcacgaaagt ctttctctgt taacgccaga tggcgcaacg
    17701 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acgattcgat
    17761 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg tgcgacggta
    17821 gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga cgatattctt
    17881 cacgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggcgga acgccacggg
    17941 ttagtgactc gtcctgctgg cgaagcgatg gcattgggaa tcaaagaagt gctgacgctg
    18001 gaaccatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgttg
    18061 ttcagcggcg ggatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa
    18121 acgctctcgt tagggattgt ttgcccgctc tcctccctta cgcaaagtag tgttccggca
    18181 agcgagctgc tgactcgctt taaaacgcat ccggcagtgc gcccgcttat caaaaacacg
    18241 gaattactgg agtatggtgc gcatctggtg ccagaaggtg gcctgcgcag tatgccggtg
    18301 caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc
    18361 ggaatttccg tgcgcggcat ggatatggcg ctaactggcg cgcaggcggc ggcacaaacg
    18421 ctgataagcg cctgccaaca ccgcgagccg caaaatctgt ttccgcttta tcatcacaac
    18481 gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc ggcgcttttg
    18541 caacgtcctg gctggtatcg ggtgtggcct gggttaatgc aggatatttc ccgtgattta
    18601 tgggatcagg ggaatacacc tgttccaccg ctgcgccagt tgctctggcg tcatttacgt
    18661 cgtcacggcc tgtggcatct gacgggcgat gttatcagga gtctgcgatg tctgtagccc
    18721 gtaatctctg gcgcgctgct gatgcgccgc acattgttcc gactgactcc gttgagcgcc
    18781 agacggcaca acggttgatt aacgcctgtc cagcaggtct tttttcgctc acacctgaag
    18841 gtggcttacg tgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg
    18901 acgaatcaac actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgct
    18961 ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca gaatccggtg
    19021 attgctgccg ttaaagataa tgccagcctg caactggcaa tcgactctga atgccagttt
    19081 atttccgtgt tgtacggcaa tatctgcacc attagtaata tcgttaaaaa gattaagaac
    19141 gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtg
    19201 gtgattcagt ttttgaaact ggtaaccgag gcggacggaa ttatcagtac caaagcctca
    19261 atgctgaaag cagcaagagc ggaaggtttt ttctgtattc atcgactgtt tattgttgat
    19321 tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag
    19381 atcctgccag gctgcatgcc taaagtgctg ggctgggtga cggagaaaat ccgccaaccg
    19441 ctgattgctg gcgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt
    19501 gttgtggcgc tttccaccac gaatactggg gtctggacgt tagcgaaaaa attgctttga
    19561 cgggataatc gtaacaagtt gaatttggtg tgatttgtag gctgcacgcc acatccgaca
    19621 ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc cagcccagaa
    19681 ccatcggcag gatcatccgg cagcacgtta ttggcaatct gcgtgaatgt ttgtcaccag
    19741 tgcctccagc accactttcc agtcatcaac tacgccaaca tctgcctgtg aaaataccgc
    19801 tgcactggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa
    19861 cgctgctgcg ccggaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga
    19921 aataccaatc actttttcgg catcaacgcc accgttcatc actcgtgccc gactgtagcc
    19981 cacttcggct cccatctttt ctgccagcat agcgatttcc tggttatctg cctctccgcc
    20041 ctgccccacc accagcaccc gtctggcttc agctaatggg tcgcggataa catttttcag
    20101 attttctatg ctaaccagcc agtcgggaag cacacctggg acaatgttca gttgctgcat
    20161 cccagagggc aacgtggcgt ttttatctgc tcccgcctga cgtgccagcg aaaggcatag
    20221 cgggcgtttg tctgtttgca gcgtcgccgt caacgcattt ccccagtggg atttgcgcac
    20281 gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca
    20341 ggccagccgg gtcgacagtt catcgccaaa cgcgcccgga ggaaaaagga caacgtccgc
    20401 aggcgttcgt tgccactgtt ccaccagtgc atccagcacc tgttccgcaa ccatcggctg
    20461 tggttctatt tgccaatgtg ccagggtaca accgctgaaa tcctgcgccg ccagccagct
    20521 cgctattgcg gcatggtcct gatttattgt cacaattacg atattcatgg ctgcatcctt
    20581 tggcgtaaat agtcctgcca tagcttttgc gctttttctg ctaccgtttg cccaccaatc
    20641 agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcaat
    20701 tcgacagcaa cagttttacg gataattgct gctttccccg cagccattcg ctggcgcatt
    20761 cccggaactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga
    20821 acccggcaac agcgcacccc atgttcagta cgctgttcga gggtgataaa cggtgcgtcg
    20881 agagtaaagc gctccacctg ggtgaagcag ggccagacca gcatttccgc cagcaaaaat
    20941 ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga
    21001 ttctgacgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct
    21061 gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag
    21121 tgcaacgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt
    21181 ctctgcgcca acagcagcgc agctgcggcc tgttcatcag caccgagtaa acttcgcaga
    21241 agcgaaacat ccggtccgct attaccctga gccgccgcct gccactcttt ttccgccagc
    21301 attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt
    21361 tcccgccgcc accagcggga gtgctttggt ttctggtgcc catagccagg tcacaacaaa
    21421 accaaccaac aacactgccg ccaggagtaa taaggtggct tgcattcccc actgcgccag
    21481 cacccacggc agcaggccag tacttaccgc cgcccccaac cgactcatgg cggtggcaaa
    21541 accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag
    21601 attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact
    21661 gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa aacttcccag
    21721 caaaaacttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg cgcccacaat
    21781 taacaacgca ttaagcatca gactggcagt cagcgcatct tccagtccaa tagtctgggc
    21841 gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga caaagaagac
    21901 gctgttaaac gctgtgcgcc gccagtagcg cgaagagaac aaggttttga tgtgtttatg
    21961 ggtcgccgtt gccacttcat cgcccagtaa aacatgcggg ccaaaatagc gatgaacgat
    22021 agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggcgatt ccggcgtgcc
    22081 ccaacgtagc agcgtaatca gcaaggctgg taacgccgct gatgccagca gccagcgcca
    22141 ggcctccggg ctttcggaaa taaagtgatg tccggcaata cttgccagca catagcctac
    22201 ggtccacacc acactgaatg cgcccagcaa aataccgcga tggcggcgcg gggaaaattc
    22261 agccagcaag gtgtgaccga ctgagtaatc gcctcccaga ccaatgccga tcaaaatgcg
    22321 taatccaata agatgctctg gcgtggtggc aaaaaattgc aagaacgaag cgagcgtaat
    22381 cagcataaaa ctgaaggtga agattttttg ccgcccgata tggtcggaga tccaccccaa
    22441 aaccaggctg ccaagaaaca aaccaagtaa cgccgagccg ccgatcattc ccgccataaa
    22501 cggcgtcagt tgcatggcgg gcgtaagctg aataatggca taaccaatga cgccgagaac
    22561 ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca attcgacagt gaaaacggtt
    22621 aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt cattgtccgg
    22681 taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgcaccgtca tcgcggaacg
    22741 ctgcctgaca cattattttt caatcggata gatagtaccc gtgttcataa tgccattagg
    22801 atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct
    22861 ccagtgaacg cgatgtttac cgataccgtg gtgatgcacc atcgaaccgc cgaggcgaat
    22921 ggtttcttcg cagatgattt tgttgagcgg attgtggtac ttgtcgattt cctcttccgg
    22981 cttacagtca acgacgttgt agtcgtagac gaagtacatg ttggtgccgt tctgatagct
    23041 atgagaggag tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact cggtacgaat
    23101 acggttaata acgctttcgt agatttcgtg aatgcagctc cagcagccgg acacttcagt
    23161 ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca ctttatccgg
    23221 tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc gctggcactg
    23281 cgggtagcgc gccacgattt cagcaatccc ttcacccgtc gccttcgcaa tgcgagggtt
    23341 accttcagcc ataaagatca gcacgcattt gccgtcggca aaatgggtga agtgttgggt
    23401 gccatcttca gcatcataca aacgagcaat cgacggacga tacccttcca ccatgatttc
    23461 acgcaggatg ttgaagccgg ttttcatatc ttccaggata tagccgtaga agagattgtt
    23521 ttccggcgta aatttgaaga ttttcactgt gacttcagtg atatagcaca gtgcaccttc
    23581 gttgccgatg atgatatgac gaatgtccgg gccagccgcg cgacgtggca cgtttttaat
    23641 gcgtgtgacg gtgccatctg ccagcaccgc ttccagacca acgaccatat cttcgattgc
    23701 gccgtagagt gtggagaact gcccgatact gcgggtcgcc accaagccgc ccatctgcgc
    23761 cagcggcttt gactgcggag aatgccccgt ggtgtaacct ttttcgcgca atgcgttttc
    23821 cagcacctcc agcggaacgc cacattgcgc cgtggcctgc atattctcaa tatcaatatt
    23881 aatgatttga ttcatggcgg agccgtcgag caccaccgag ttttccacaa cggtttccag
    23941 tccaccttcg gtggcggaag caccggtacg tggcacaccg ttaattttgt gcgcattcat
    24001 aaaattcagc acacgggata cttgttctgt ggaaccgagt tttacgaccg ctgccggaat
    24061 cggcaaagta taaattccat gaatatccgg aaatttacga aaacggtcaa tactgttttt
    24121 ctttaatacc gtttcatcgg taataacgcg atctgcacca acaatttcct ttagctggtc
    24181 gacaatcgc
//
LOCUS       DAJOTB010000068        24106 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJOTB010000068 DAJOTB010000000
VERSION     DAJOTB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.068"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            25..954
                     /gene="aaeR"
                     /locus_tag="OHN28_002619"
     CDS             25..954
                     /gene="aaeR"
                     /locus_tag="OHN28_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCQ2297998.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1076..2521)
                     /gene="tldD"
                     /locus_tag="OHN28_002620"
     CDS             complement(1076..2521)
                     /gene="tldD"
                     /locus_tag="OHN28_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCQ2297999.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(2677..6477)
                     /gene="yhdP"
                     /locus_tag="OHN28_002621"
     CDS             complement(2677..6477)
                     /gene="yhdP"
                     /locus_tag="OHN28_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCQ2298000.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6545..8014)
                     /gene="rng"
                     /locus_tag="OHN28_002622"
     CDS             complement(6545..8014)
                     /gene="rng"
                     /locus_tag="OHN28_002622"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCQ2298001.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8004..8597)
                     /gene="yhdE"
                     /locus_tag="OHN28_002623"
     CDS             complement(8004..8597)
                     /gene="yhdE"
                     /locus_tag="OHN28_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCQ2298002.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8606..9094)
                     /gene="mreD"
                     /locus_tag="OHN28_002624"
     CDS             complement(8606..9094)
                     /gene="mreD"
                     /locus_tag="OHN28_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCQ2298003.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9094..10197)
                     /gene="mreC"
                     /locus_tag="OHN28_002625"
     CDS             complement(9094..10197)
                     /gene="mreC"
                     /locus_tag="OHN28_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCQ2298004.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10263..11306)
                     /gene="mreB"
                     /locus_tag="OHN28_002626"
     CDS             complement(10263..11306)
                     /gene="mreB"
                     /locus_tag="OHN28_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCQ2298005.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11611..13551)
                     /gene="csrD"
                     /locus_tag="OHN28_002627"
     CDS             complement(11611..13551)
                     /gene="csrD"
                     /locus_tag="OHN28_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCQ2298006.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13703..14677
                     /locus_tag="OHN28_002628"
     CDS             13703..14677
                     /locus_tag="OHN28_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2298007.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            15654..16124
                     /gene="accB"
                     /locus_tag="OHN28_002629"
                     /gene_synonym="fabE"
     CDS             15654..16124
                     /gene="accB"
                     /locus_tag="OHN28_002629"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCQ2298008.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16135..17484
                     /gene="accC"
                     /locus_tag="OHN28_002630"
     CDS             16135..17484
                     /gene="accC"
                     /locus_tag="OHN28_002630"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCQ2298009.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17576..18622)
                     /locus_tag="OHN28_002631"
     CDS             complement(17576..18622)
                     /locus_tag="OHN28_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCQ2298010.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(18619..19581)
                     /locus_tag="OHN28_002632"
     CDS             complement(18619..19581)
                     /locus_tag="OHN28_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2298011.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(19603..20592)
                     /locus_tag="OHN28_002633"
     CDS             complement(19603..20592)
                     /locus_tag="OHN28_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2298012.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(20593..22092)
                     /locus_tag="OHN28_002634"
     CDS             complement(20593..22092)
                     /locus_tag="OHN28_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298013.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22153..23043)
                     /locus_tag="OHN28_002635"
     CDS             complement(22153..23043)
                     /locus_tag="OHN28_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2298014.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(23079..23933)
                     /locus_tag="OHN28_002636"
     CDS             complement(23079..23933)
                     /locus_tag="OHN28_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCQ2298015.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         6018 a         6480 c         5721 g         5887 t
ORIGIN      
        1 atcactttta agtcagagtg aataatggaa cgactaaaac gcatgtcggt gtttgccaaa
       61 gtagttgaat ttggctcttt taccgccgcc gccagacagc tacagatgag cgtttcgtcc
      121 atcagtcaga cggtgtcaaa actggaagat gagttgcagg taaagctgtt aaaccgtagc
      181 acacgcagca ttggcctgac cgaagccggt agaatttact accagggctg ccgtcggatg
      241 ctccatgaag tgcaggatgt tcatgagcaa ctgtatgcct tcaataacac ccccatcggg
      301 acgctgcgca ttggctgttc ttcaactatg gcacaaaatg ttctcgccgg gctgacagca
      361 aaaatgctga aagaataccc aggtttgagc gtcaatctgg ttaccggaat tcctgccccc
      421 gacctgattg ccgacggtct ggatgtggtg atccgcgtcg gcgcgttgca agattccagc
      481 ctgttttccc gccgtctggg cgcgatgcca atggtggtgt gcgccgcaaa aagctatctc
      541 acacaatacg gcataccgga aaaacccgcc gatttaagta gtcactcatg gcttgaatac
      601 agcgtgcggc ccgacaatga atttgaactg atcgcaccgg aagggatctc gactcgcctg
      661 atcccacaag gaagatttgt gactaatgat ccgatgacgc tggtgcgctg gctgacggcg
      721 ggtgccggga tcgcctacgt gccgctgatg tgggtgatca acgagatcaa tcgtggggag
      781 ctggagatcc tgctgccacg ttaccagtca gatccacgcc cggtttatgc gttatatacc
      841 gaaaaagata agctgccgct gaaggtacag gtcgtgatca actcgctgac ggattatttt
      901 gttgaggtcg gtaaattgtt tcaggagatg cacgggcgcg ggaaagagaa gtaattaata
      961 ttgtactgtt acgttgtaca aacctgtgcc gacgggttcc cctaacccca gaggggcgag
     1021 gggaccgttc gtgcacggag aaggattaat tatccttctg aaaatactga aattattacg
     1081 cagtaccgcc aacagtcagg ttattaactt tcagcgttgg ctggcccacg ccaaccggca
     1141 aactttgccc ttctttaccg cagacaccca cgccgttatc cagtttcagg tcgttgccaa
     1201 ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat caacgttgcg cctttcaccg
     1261 gcttagttac tttaccgttt tcaatcagat acgcttctga ggtggagaaa acgaatttgc
     1321 cggaggtgat atccacctga ccgccaccaa agttcggtgc atagataccg tactcaacgg
     1381 attcaataat ttcctgcggg gtcgatttac ccggcagcat ataggtgttg gtcatacgcg
     1441 gcatgggcag atgggcgtag gattcacggc gaccgttgcc agtcggcgtc atccccatca
     1501 aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat gccgttctca atcagcacgt
     1561 tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga accacggcga tcgaccatcg
     1621 tgccatcatc aaccacggta cacagttctg aagccaccaa ctccccgacc tgtccactaa
     1681 atactgaagt gccacggcgg ttgaagtcgc cttccagacc gtgacccacc gcttcatgca
     1741 acagcacgcc cggccaacct gcgccaagta ctaccggcat ggtgcccgct ggtgcagcaa
     1801 cggcagaaag attgaccagc gccatacgca ctgcttcttt tgcccacgca tccgcacgga
     1861 cttcgccgtc gagatcggca aggaagaact cataaccaaa acgaccgccg ccaccactgg
     1921 caccgcgttc gcgtttgcca tcttcttcga ccaaaacgct cacggaaaga cgcaccagcg
     1981 gacggacatc cgccgctagt gtgccgtcgg tggccgcaac cagaattaat tcatagacac
     2041 cactgaggct ggcagtcact tcctgtacgc gcttgtccgc ttcgcgggca accttatcga
     2101 cgcgacgcag gatatccaac ttctcttcac ggctcatgct ttgcagcgga tctaccgagg
     2161 tatataacgg gctatgctct accgcgccca gcgtctgtac tttgccatca ccactatcac
     2221 ggacgatggt gcgcgccgct tgtgcactct gttccagcgc cagcaggctg atttggtcag
     2281 cataagcaaa tccggttttt tcaccactga ttgcacgcac accaacgccc tgatcgatgt
     2341 tgtaagaacc atctttaata atacggtctt ctaaaaccca ggattcgtga tagctcgact
     2401 gaaaatagag atcgccataa tcaaggcgac gttcggccag ttgaccgagg atcgcgaaca
     2461 agtcctgatg tttcaggccg ttcgccgcta gcaattgttc acttaccagg ttcagactca
     2521 tcgtttttgc tactcgttag ttactgcagt agaggatttt ttacggctgc cggatgcggc
     2581 gtgaacgccc tgtccggcct acggttatgt tctggctcgt tggcctttgg caacgattat
     2641 catatgagat tggggcaatt acgcgccctc gtcaaatcat tgcgcttttt ctttacgcgg
     2701 ttgtcgcaac acttcgttga tttgcggatc gtccagcgga cccgaaatgt gatagcgcaa
     2761 aatggagact ttgctccaca gcggtcccag cactttactg gcggcaaaca ctgccgcgcc
     2821 aacaatgggg ttaaccgcaa aggccgcagc cacacccacc gtcgcagaaa tctctggtgc
     2881 gacaaccgct tccatattca ggtcgcgacg taccagattt accgaccctt tcatggcgat
     2941 gtccgcttcc aggccatcca ccagcgtgtc gtcggtgtgc ataacgccgt ctttaatcca
     3001 cgcggtgctg cgaatggagt caaaatagaa cccttcgcca aaagtgtctc tgaaatcaaa
     3061 acgcagctta cgcatcaggg catctacgct caataagcgc agcaattgcc cggcatgtcc
     3121 ggtattgatt tcggtaattt cgcctttacc cagttgagta tgaatgatgc cattcaacgt
     3181 cgcctcatct ggctgccagg gtgctttgcg ccagtgtaaa tcgtaatcca cattaaatga
     3241 cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg gcggcatcaa ttttctgccc
     3301 gcgcagtttt cctttcagcg aggttcgttc attccccgga ttgttaaccc attcaccatc
     3361 ggcagtgagc cgcgcaaaac cagtatcaat cagtccatta gtcagcgtta acgtatcgcc
     3421 agaaatggtg atatcactgt caatgcgacc gaatttttgc ccccagaacc agcactctgc
     3481 gcatcgtatt tgtgcgtctg gccagccacg gaagctaatg cgttccgttg tcgggaaagg
     3541 tgatgactgt gttgaatcac cacgagtttt cgccacgctt gggttgtaat aaagatattt
     3601 gatattcgcc agccacggcg cgttattacg catcgccagc gtggcgttga tttcacgccc
     3661 ttgcgcctca accagggtgc catttgccgt cggttgcgaa acaatactca ggttattcca
     3721 ttgctgattt cccagcgaca acataggcgt acgtaacgtt atgtgttgtg ggaaactcgc
     3781 tgcaccaccg acactctccg ccgcgccttt ctggaacagg gccagccact cggcaccatt
     3841 catcggcggc atattgagtt caacgccact ttgttccggc aacggcggga gcgttttact
     3901 gtctgccgcc caaatagcac gatcgagcgt cagcttttga ccgagcaacc agcggctatt
     3961 gaaatagtta tccgcaccag cctgtccggt taattcaaag ctgttgagat tgccatcaac
     4021 cttaacgttt accgatagtg gttcacccgc aggtttggct aacggtgaag gtaagtgact
     4081 gctcacattc ttcaaatcgc cgttcagctc tacgttatac gtcgcgccag catgataagg
     4141 cagctcaatg cccactttac catcccacgc cacgctgcca cttaatgctt cgttcaccgc
     4201 tgcaggcaga acgccggttt tcgctggttg ccagttaccg ttgagattca ccgcgacctg
     4261 gtaggctttt gccccttctt tggtggaaaa atccacgttc aacggctgat taaaccagct
     4321 tgctgtcagt ggttcacttt gcagatcgcc attgataaag ctgaatttac cgctcaaatt
     4381 tttcagggtg ctgtcgagtg gtttgataaa cagactgtta ttacgcagcg tcacttcacc
     4441 tttcgcggtt accaactcgc cgttcagcgg gatatcaaga tgtaagcgag cattcacatc
     4501 gccgtcgagc tggagttctt gcagggtcgc acccagggaa tctttcagcg gtgtctcatc
     4561 aaagtaaggg ccaacggctt tacccggacc tttaatgtca gcgtcaatca gcagtttttc
     4621 ttttgagtag tcagggatca ctgcggtgag attactcgcg cgcacgccgc ccagattaac
     4681 gccatcggtt ttcatccata aaccgtcgtt aataaagtcc agttcaatat caaggttagt
     4741 taatgcaggc cagtccggct ggaaggcaaa cttcgcgttg cgcagcggca ccagcacttc
     4801 aaactgacct tcgttgtgtt tataggggaa gagttgcgga ttgccaccat aaaccagcgt
     4861 cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag taatcaacca ggtctttacc
     4921 catcaagttt tccgggaaat agcgccaggc ttgtgaacca tcatcggtac tgatgccagc
     4981 cagaataccc agccagggtt catcgttagc aggttgcagg taacgaaaac cgccgcgcgc
     5041 atggacggct ttggctttaa cgtcaatatt acgcccatcc agctggaaac ctttgtcatt
     5101 gttcagccag cttatcgttg cctggccgtc ggcgatttcc agtggcgcac ggaataccgt
     5161 ttcgtaaggc atctttgcct gcttcatcga cgcagtaagc aaaccatttt ctacgctgcc
     5221 ggaaagcgtc ccggagaagt gttccgcacc cggtaataat ttccattgct tccaggccag
     5281 atcgctccac gatgcctgaa aacgggtctt gtctgccgcc tgaagcggga tatccagcgc
     5341 cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa acatcaccca gtgcaggtga
     5401 aagtttcgcg accagcgggc gtacgccctc cagccctgcc agctccagat tactggcacg
     5461 aatccggagt tcgtcactgc gtttattgtc tttgccgcca acgtcctgtt ccggtatcca
     5521 ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc atcgtgatcc gtgtatcggg
     5581 aatagagaac tgccatcccg gattttcacg cgtaatatgc gcagtcagat tatccaccga
     5641 cagcgtatgc gtttccttct cgcccaacca gctggcaccg ccctgtttca gccagacgtc
     5701 accgccggtt acatcgcctt tgtcgatcgt catccagcct tcaagggaga actgtgccgt
     5761 ttccagcgca atattgtcct gcatccattt accgagccac ggcttcaggt cgatgtcatc
     5821 cgcctggagc cagacgcgac cattgcttaa caacccctca tcatcgcgca aatccatacg
     5881 cacctgcatc acgccgtgct gtccggtaag gctggagagg cttaccaggc cttccgcacg
     5941 gtgtcgacgt ggatcgttca gccaggtgag ctgtgggatc gccagctcgg cgcgctgacc
     6001 ggatggcgtc aggaaactga cttcactgtc gcggagatcg aaatggtcaa attgacgaag
     6061 aaacagatcg ctgatgtgac tggcttccag gctatcatta ccaccgccgc tggtgataga
     6121 agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg cgaaactgcc agcgcatatg
     6181 taacaggctc tgccagacat ccagcgccag ggtaacgcgt ttaaccgaaa attcgccgcc
     6241 atcttttagt tctgcacgga tgtcgtgcgc ttcaagcgtc ggaccaaaat tctgccagct
     6301 ggctgagagc tgactggctt ctaccggcat gccagtcgcg gattctattt tgttgaggat
     6361 ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc aggccgctaa ccagcagggc
     6421 agcgatcaca acgagcgcgg ctccagtaag cagtaaaatc cccggcaatc gcctcacccg
     6481 tcactccttg tctgctaaaa atgtgactca aaaacccttt gccggatggc ggcccagcat
     6541 ctgtttacat cattacgacg tcaaactgct cctggttata gagcggttca atttgtactt
     6601 taacctgttt gccaacgaaa atttccactt ccgccagcga gtgtgactct tcgcctttca
     6661 aggcttcggc tactgccgga gaagcataga ccaggaaacg gtcggagtcg taagcatggt
     6721 ggacacgaac aatctcgcgc atgatttcat agcataccgt ttccacggtt ttcaccgttc
     6781 cgcgaccgtg gcaggttggg cattcgttac acagtacgtg ctcaatgctt tcgcgggtgc
     6841 gtttacgcgt catctccacc agccccagcg ccgaaaaacc attaacgctg gttttcaccc
     6901 gatctttgct caacgcctgc tccagcgagt gcagcacgcg gcgacggtga tcttcattat
     6961 tcatatcgat gaaatcaata atgataatcc cgcccagatt gcgcaaccgt aactggcgag
     7021 cgatagcctg cgtcgcttca atattggtat tgaaaatggt gtcgtccaga ttgcgatgac
     7081 cgacaaacgc cccggtattg atgtccacgg tggtcatcgc ttcggtctgg tcgataatca
     7141 gataaccacc ggatttcagt tctactttgc gttccagcgc tcgctggatt tcgttttcga
     7201 catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg ctccagcttg ctggtcatct
     7261 cgggaatgta ctctgaggta aactccagca atgcttcgta agtcaggcgt gagtcaacgc
     7321 gaatgcggtc gagttcggcg tcggcgaaat cacgcagaac acgctgcgcc agcgccagtt
     7381 cgccgtacag ctgataacgg gtctgcgggc gttttttacg ctccataact ttggtccaga
     7441 cgcgtttcag ataagcagca tcggaggcca gttcggcctc gccaacccct tccgctgcag
     7501 tacggatgat aaacccgccc tgctcgtcgc aatactctgc gaccactttt ttcaggcgtt
     7561 cacgttctga ttcgctttca attcgttggg ataccccaac gtgagaagcc cctggcataa
     7621 acaccagata gcgagaaggc agcgtgatat cggtggtcag gcgcgcaccc ttagtgccaa
     7681 gcggatcttt caccacctgc accatcagat cttgcccctg acggaccagt tccgagatgt
     7741 cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca ttcggtgtgc ggcatgatgt
     7801 cagatgcatg aagaaacgcg gctttatcca gcccaatatc tacaaaagcc gcctgcatac
     7861 ccggaagtac acgacttaca cgacccttgt agatattgcc tactattccg cgtcgcgcct
     7921 cacgttcaat atgaatttcc tgcagaatac cgccatcaat atacgccact cgagtttccg
     7981 aaggcgttac gtttaccaac aattcagccg tcatgtttat cccttttctc acgcagtgcg
     8041 ttaaaattac ttaataattc atacgtttca accagcggta agccgactac ggcgtgaaag
     8101 ctgccattta tcttcctgac aaaacagcca cccagcccct gaataccgta tgcacctgct
     8161 ttatctaacg gttcgccgct ggcgacatag cccgcgatgt cttcgtctgt taacgttctg
     8221 aaagtcacat cggtgaccac caggcaatcg agaatgtgct ggctgtcggc caacgccact
     8281 gccgtcatca cctgatgggt ctgacccgat aatttgcgca acatctgcgc cgcatgctct
     8341 gcgtcgcgcg gtttctcgag cacttctcca ttcaggataa cgatagtatc cgcacccagc
     8401 accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg ctttctcgcg cgccagacgc
     8461 acaacatact gctgcgcgct ctcctgcggc tgacgctgct cctcaatgcc cgtaacaata
     8521 cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac gacgcggaga accggaagct
     8581 aaatacagag aagtcataga aacctttatt gcactgcaaa ctgctgacgg actttacgca
     8641 tcagcaagaa aatccacggc cagagaacgc cattgactac actactccag aacacttccg
     8701 gtctgaaaga gacgttaatc actaaaaact ctgcccagaa aacaataata tccaccacca
     8761 gcgaaagcaa catgacgacc agcgcctgct gccataatgc gaggttgcgg aaaagctggt
     8821 atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa tacgcgtacg ccaagcgtcg
     8881 agccgctgat cagatccagt atggcaccca tcacaaaacc tgtgcccaca tttacgcgat
     8941 ggggcaaggc caggatccaa tacaacaaga tgagtaacac ccagtttggc cggaaaacaa
     9001 tcaggttgtc cggccatggc atgatttgca gcaacagcgc aatgaggaaa gagagccaga
     9061 ttacccagcg tccctggcta cgatagctcg ccactattgt cctcccggcg cacgcgcagg
     9121 cggttgagca ccactttgcg gcggcgtagc cctttgtgga gagcgattag cagcgggctg
     9181 tgtgggcgca gcaggcgcag taactgcatt tcctgtcgcc ggttgctgcg gagtcggctg
     9241 agtgatcccc gttgccggtt caggtaactt tggccccatc gcgtctggcg aaggcaatac
     9301 ctgcggcatc atctgcatca gacgttcatt agcaacacga tgcacctctt ccggcgtcat
     9361 cgggttagcg ccgttacgat ccgcgcccca cagcagcagc agataacgca aacgttgcag
     9421 tcctgcagtc ggacgcgcct gaatcacagt ataagcgcgc tgggtatcga gttttacgga
     9481 agagacaacc gcgaccggat agccttccgg gaaacgaccg cccagaccgg aagtcaccag
     9541 cacatcacca acacgaatat ccgtattcgc tggcagatgc tcaagctgca aatcatccgt
     9601 acaaccgtta ccggctgcaa ttacgcggat atcgttgcgc agcacctgga ttggcagcgc
     9661 gtgggtcgca tcacaaatca gcagcacgcg gctggtcagt ttagcgacgg ccaccacctg
     9721 accaacaaca cctttgtcgc tgataaccgg ctggccttca taaacgccat taacgctacc
     9781 tttatcgata acaacttgat cgctataagg atcgttaacc gtggagataa cctgagtcac
     9841 cattttctgc tcatcctgac gcagcgggga acccagcagc tcgcgcagac gcgcgttctc
     9901 ctgtttgtat tgtccaagca tcagcagttc actgtttttc agcaacagtt cctgacgtaa
     9961 cgcccggttt tcaagttcta gttggtcacg cgaggccagc gtctgcgata cgccatccag
    10021 caattcacga ggagcattgg aaacaaagta gaaaggactg acggcggtat ccatataagt
    10081 acggatttga ctgaacgtcc ccaggcggct gtcggcaata ataatgccga gcgccaccag
    10141 caccgccaga ataaggcgaa tctgtagcga cgggccacgg ctaaaaattg gcttcataag
    10201 ttatgcgtat tctcgtatca gaccaggcag ggtaaacaga cacttcccct gcctgcatcc
    10261 gattactctt cgctgaacag gtcgccgccg tgcatgtcga tcatttccag cgctttgccg
    10321 ccaccgcgcg ccacacaggt cagcgggtct tcagcaacaa cgactggaat gccggtttct
    10381 tccattaaca aacggtcaag gttacgcagc agtgcgccac caccggtgag caccatgccg
    10441 cgctcggaga tgtcggaagc cagttccggc gggcactgtt ccagtgcaac cattaccgcg
    10501 ctcacaatac cggtcagcgg ttcctgcagt gcttcgagga tttcgttgga gttcagggta
    10561 aaaccgcgtg gaacaccttc tgcaaggtta cggccacgaa cttcgatttc acggacttca
    10621 tcgcccgggt aagccgaacc gatttcgtgc ttgatacgtt ctgcggtggc ttcaccgatc
    10681 agagaaccgt aattacgacg tacatagttg atgatagctt cgtcgaaacg gtcaccacca
    10741 atgcgcacgg aagaggagta aaccacgccg ttcaaggaga taacagcaac ttcagtagta
    10801 ccaccgccga tatcaaccac catagaaccg gtcgcttcag aaaccggcag gccagcacca
    10861 attgcggcag ccatcggttc ttcaatcagg aagacttcac gggcaccagc gccctgcgcg
    10921 gattcacgaa ttgcgcggcg ttcaacctgg gtcgcgccaa ccggcacaca aaccagaacg
    10981 cgcgggcttg gacgcataaa gctgttgctg tgcacttgtt tgatgaagtg ctggagcatt
    11041 ttttcagtca cgaagaagtc ggcgataacg ccgtctttca ttgggcgaat ggcagcaata
    11101 ttgcccggcg tacggcccag catctgcttc gcgtcatgac ctactgcagc tacgcttttc
    11161 ggtgaaccgg cacgatcctg acgaatggcc accacggaag gctcattcaa tacgatgcct
    11221 tgtcctttta cataaatgag ggtattcgca gtacccaggt caatggacaa gtcattggaa
    11281 aacatgccac gaaatttttt caacatacta agggataatc ctgaaagctg gggcggaaaa
    11341 gaaaatccgc ttactttacc aaccacacgc agcagcgaca aggcgcaaaa atcatctgct
    11401 acggtgaaaa ttagtgcagt tcgtttcctt tgttacaaat ctctgcctga gtccagaaag
    11461 gcttaatgca tcagcagcat tcctcgcctg tttgcaaccg cgtaaggtca ttcatctgca
    11521 tatgtgctgc aacaatctgg cgagcagaca agcacactcc catgagacgc agcgcgcgtt
    11581 attctacgtg aaaacggatt aaacggcagg ttaaaccgag tatctttgtg aatatttttt
    11641 cacgttagta tcaagtggct gtgaggacgc gaaaaaatcc ccttgcccac ctgtaacacc
    11701 gcgctgaatc agggtctgcc actcgcttcg cgaacgcacg ccggtggcgt aaacctgggt
    11761 gctggtcccg gagcaggctt ccaccaggct ttgaaccagc agctggttct ccgttcgctt
    11821 ctcaatgttt ctgaccagcc ccggatggag cttgagtaac tcaacattaa gttctttgat
    11881 ccaactggtg cttaccagcg tcaaaccagc ctggttgacg gctacccgta cccctaaagc
    11941 attcactaaa cgaataacag gttgtaagcg actgatatgt tgaccaacat cggcctctgc
    12001 aagttcaata attatgcgtc ttcgttgcga tttttcacat tgcattaacg tatcgcgcag
    12061 ccaacgctga aaacgcgggc gaatcagcga ctcaacggta acctgaatcg ccagattttc
    12121 ctctggccag taacgcaata gtggaataag acggctgatt agcaagcggt catactcttc
    12181 cgataaacca aactgcaaga ccatcggcat atactctgcc gagctaacct cttcattacc
    12241 atcgaagatg cggcacatga gttcacgatg atgaacccgg ccttcgcgag taaccgccgg
    12301 tttttgataa aggcgcgggc cgccgcgact gagcatttgc tcgataagcg tacgccagcg
    12361 aacattaccg cgtccttttt caggcaacga gtcatcgtaa atagcccagc tattgccgcc
    12421 ctgcaatccc gcattacgcg tggcagactc tgcatgttcc attacctgct cggtatcctg
    12481 accactacgc caggcacaga taccaatgtg gatcatatcg tcgcgatcga gcattttatt
    12541 gttcggcaag gtatcgacgg ctttgattaa ctgactggcg atgctctctg cctcttttag
    12601 cgtccggtgc ggtaacagcg cagcaaaatc actgcgatgg taacgcgcca gcagtgcgcc
    12661 agggtagcgc atcataaatg tcgatagcag attcgtcaga gagaagaatt gttcttcaac
    12721 caggctgtgc ccccaggtat cactcaacat attgaaatcc ggcagacgaa tcatcatcac
    12781 gatcccgtgg gtacctactt tctcttgatc ttccagtaac gttgctaact gattatcgaa
    12841 aaagagtcgg ttattgaggc cggttttcat gtcctgggcg gcataagagc ggatcagcgt
    12901 atcaagacgg ctgtgttgtt cgcgtgcgtt ctgaatttca caaagcagcg tatccagcgc
    12961 actgctggtt ctgggcggcc attcatagat ggttcccaac acattagagc cacgctcacc
    13021 gtttaagata cgagtagccc gggtttccag caattcttgc ccggcaagtt gccgttgtaa
    13081 ccagcgtacc gccaggaaga gcataagaat gataaagcca atcgcccccg tgagcggcgc
    13141 ggtggtcatt aaagaatgga aatagttgcc catcggatcc tgataaacca gacgcagcga
    13201 catccccgga tgctttatca acggaacgct cagttcacga aacagatcgt tggtgccaac
    13261 cggacgataa ctaccatttc tggccagggt ataaacctgc ttatcaccat ggagcaggtc
    13321 tacacgaacg atatctgccg acatcattaa ttcggtaatt tgtggcctta atgtgctgaa
    13381 gtcattcgac agtaggtggg tatcaatcgc cgtcgccacc gcctgaacac gatgactaaa
    13441 cttatactga atggcgttgt agaaacttag cgaacagccc agcaaagtca caaaaattgt
    13501 taacccggtg agcagcgtga caaaggccga aaatttcgtc gttaatctca tccttgtgtt
    13561 aactccgata gtgaggaagc gggcatacta gcaaatcaga tttatctcgc aatttattgc
    13621 gcttcatcgg ctttgctttt ccattagcga gtatagtctt cagaaattat tttccaatcc
    13681 atcatgcaca tgaggaccac ttatgcaggc gttactttta gaacagcaag acggcaaaac
    13741 tctcgcatca gtacagactc tggacgaaag tcgcctgccg gaaggcgatg tcacggttga
    13801 tgttcactgg tcgagcctga actacaaaga tgcgctggcg attaccggta agggaaaaat
    13861 catccgtaat tttccgatga ttcctgggat cgattttgcc ggaactgtac gcaccagcga
    13921 agatccgcgt tttcatgccg gtcaggaggt gttactcact ggctggggcg ttggtgaaaa
    13981 ccactggggt gggctggcgg aacaggcgcg agtgaaaggt gactggctgg ttgccatgcc
    14041 gcaagggctg gacgcgcgta aagcaatgat tatcggtact gccggtttta ccgccatgct
    14101 gtgtgtgatg gcgctggaag atgccggtgt tcgcccgcag gatggggaga ttgtcgtgac
    14161 gggtgccagt ggtggcgtcg gcagtaccgc cgtggcgctg ctgcataagt tgggttatca
    14221 ggtcgttgct gtttccggtc gcgaaagtac ccatgaatat ctgaaaagtt taggtgccag
    14281 ccgtattctt cctcgcgatg agtttgccga atcccgtcct ctggaaaaac aagtctgggc
    14341 tggggcaatt gacaccgttg gcgacaaagt gctggcaaaa gtgctggcgc aaatgaatta
    14401 cggcggctgc gtggcggcct gtggtctggc gggtggtttt actctgccaa ccacggtcat
    14461 gccatttatt ctgcgtaatg tccgtttgca aggggtggat tcagtaatga cgccaccagc
    14521 acgccgtgca caagcctggc agcgactggt caccgattta ccggaatcat tctataccca
    14581 ggcggcaaaa gagatatctc tggcagaggc accgaagttt gccgaggcca tcattaataa
    14641 ccagatccag ggtcgcacgc tggtgaaggt taactaacca tttagcaggg aataataaga
    14701 gagggaactc attttgaaat cattattcaa tcagttaacg ggaaaagcgg ttagccggac
    14761 agctttcgtc gaacatcttg gttatgaagt tgtacaacat catccaaact ggaaagtcat
    14821 gatttcgact gaccataaat tgatgcgcat tgatgctcca ccaaacagcc attattgata
    14881 cgcctccgtc gcctgttagg tttatggtgc tttgccttgg cgacgctacg cttaatctgt
    14941 tactgatttc tggtaccatg gggggaataa tctgatttta tttgattaca aattaaccac
    15001 ttaaacctat tgaatgcgga gcattgtcag tcggttaatt ttgcgtgctt tagcattcac
    15061 atctttccgg acgatgcagt gaaaattggg taatccccag caaccgctgc gtaatgtcgt
    15121 ctatcttgtc gcgatcctgg catccctaca ttatttgtgg tctgtgaaga ttatctcatt
    15181 gcagccccca tcttcgcagg gctggcttta cagcgtttca ccttacgtta taagaagttc
    15241 cgtcgatgat ggcgctaatt tcgtgaattg tgcggcttgt tgcaaattac acggtgttga
    15301 aggttattta catgttagct gttgattatc ttccctgata agaccagtat ttagctgcca
    15361 attgctacga aatcgttata atgtgcgacc tcgtcctccc tgacgcagtt tttgcactgc
    15421 ggaaaaggtg acattggcgc aacgaaggta tattttgttt tttgccggag gatagcagca
    15481 gatcgctgca caatgtccgt caagtctaac attgacactc tggggcaaaa tagaccggcg
    15541 tcccggcctg ctggaattta tcgctatgca tacagctgtc ggggcatacg ctttacagac
    15601 ggcggtgaaa cgcctgtcac aatcacacta aacaaagagt acggaaccca ctcatggata
    15661 ttcgtaagat taaaaaactg atcgagctgg ttgaagaatc aggcatctcc gaactggaaa
    15721 tttctgaagg tgaagagtca gtacgcatta gccgtgcagc tcctgccgca agtttccctg
    15781 tgatgcaaca agcttacgct gcacctatga tgcagcagcc agctcaatct aacgcagccg
    15841 ctccggcgac cgttccttcc atggaagcgc cagcagcagc ggaaatcagt ggtcacatcg
    15901 tacgttcccc gatggttggt actttctacc gcaccccaag cccggacgca aaagcgttca
    15961 tcgaagtggg tcagaaagta aacgtgggcg ataccctgtg catcgttgaa gccatgaaaa
    16021 tgatgaacca gatcgaagcg gacaaatccg gtaccgtgaa agcaattctg gtcgaaagtg
    16081 gacaaccggt agaatttgac gagccgctgg tcgtcatcga gtaacgaggc gaacatgctg
    16141 gataaaattg ttattgccaa ccgcggcgag attgcattgc gtattcttcg tgcctgtaaa
    16201 gaactgggca tcaagactgt cgctgtgcac tccagtgcgg atcgcgatct aaaacacgta
    16261 ttactggcag atgaaacggt ctgtatcggc cctgctccgt cagtaaaaag ttatctgaac
    16321 atcccggcaa tcatcagcgc cgctgaaatc accggcgcag tagcaatcca tccgggttac
    16381 ggcttcctct ccgagaacgc caattttgcc gagcaggttg aacgctccgg ctttatcttc
    16441 atcggcccga aagcagaaac cattcgcctg atgggcgaca aagtatccgc aatcgcggcg
    16501 atgaaaaaag caggcgttcc ttgcgtaccg ggttctgacg gcccgctggg cgacgatatg
    16561 gataaaaacc gcgccattgc taaacgcatt ggttatccgg tgattatcaa agcctccggc
    16621 ggcggcggtg gtcgcggtat gcgcgtagtg cgcagcgacg ctgaactggc acaatccatc
    16681 tccatgaccc gtgcggaagc gaaagcagct ttcagcaacg atatggttta catggagaaa
    16741 tacctggaaa atcctcgcca cgtcgagatt caggtactgg ctgacggtca gggcaacgct
    16801 atctatctgg cggaacgtga ctgctccatg cagcgccgcc accagaaagt ggtcgaagaa
    16861 gcaccggctc cgggcattac cccggaactg cgtcgctaca tcggcgaacg ttgcgctaaa
    16921 gcgtgtgttg atatcggcta tcgcggtgca ggtactttcg agttcctgtt cgaaaacggc
    16981 gagttctatt tcatcgaaat gaacacccgt attcaggtag aacacccggt tactgaaatg
    17041 atcaccggcg ttgacctgat caaagaacag ctgcgtatcg ctgccggtca accgctgtcg
    17101 atcaagcaag aagaagttca cgttcgcggc catgcggtgg aatgccgtat caacgccgaa
    17161 gatccgaaca ccttcctgcc aagtccgggc aaaatcaccc gtttccacgc acctggtggt
    17221 tttggcgtac gttgggaatc tcatatctac gcgggctaca ccgtaccgcc gtactatgac
    17281 tcaatgatcg gtaagctgat ttgctacggt gaaaaccgtg acgtggcgat tgctcgcatg
    17341 aagaatgcgc tgcaggagct gatcatcgac ggtatcaaaa ccaacgttga tctgcagatc
    17401 cgcatcatga atgacgagaa cttccagcat ggtggcacta acatccacta tctggagaaa
    17461 aaactcggtc ttcaggaaaa ataatttgtg acgcgcggtg ttttcatcgc gcttagcaaa
    17521 gaacgtaatc aacgccgggc ctgttaatgc tacccggcgt ttttattgcc gttaactaac
    17581 ggtagcctgc aatttccgct ttcaacgcgc taatttcagc atcgccatca acaattccca
    17641 gctcaaagca gtatttcttt gtttcccctg gctcaataaa gggcagacgc ccctgcgctc
    17701 gttctttatc acgaccgtca ggaaaactat tactgacctc cagtcccata acatattcac
    17761 ccgcgcctaa atttttccac tggataaagt tgggtagctg catggtgtcg aatttctcgt
    17821 aaattcccca ccccagttcg gcgttcacca gcgcaacttt actcatcccc tgactgtcgc
    17881 tattcaacgt gagatagtaa acctgctcgt caaaaccggg ctgtggggta caaatctcca
    17941 gatgactggt taacccttcc gccgcatgcg gagttcgtgg cgttatgctt ttagccgaca
    18001 gcaagatttc tgcctgtggc gataataaag gccagccgta attaaagtga taaagaattt
    18061 gcagcggctg gcgggtgaaa ccatgattag tcaccttgtc ttcaatgcgc attacatttt
    18121 cgccataacg gcagcgaatg gtcctttcca gcgtcagatt ttcaccgaac aatcgtgttt
    18181 cccgtacttt gccattgatg acaaattcaa tgctgtcgtc ggtccgttct gtgcgatacc
    18241 ccacctcttc tgctggagtc gcagccaaac gcccgtgtaa ccccagcgtt tctccttcat
    18301 cctcacaggg tgtgccaatg tagctaagtc cgcaggtagt cagtaacccc gcgtaaaagc
    18361 tacgtaaaaa cccgttgccc tgagactcaa aaaaagcggg ggcgacaaca ccgttggggg
    18421 tgataaaaga gagcgcagtg tccttgtagc tggcccatgc aatatccagc gcgcgatctt
    18481 ttaccaccgt aaatgccagt cccgcaccag tacggacatc aaacgcttct gtaccctttg
    18541 ctctgccact acgtaactca tagtgtttaa ttccggcaac ctgcgagaga tcgccaatcc
    18601 acgaccaaat ttcgtttttc attgtgctgc ttcccgaatg gacatgtcat ggcgctggat
    18661 gaacgtttcg atttccataa ggcgagacgt tccctccatt gggccacgtc gggttaccgc
    18721 cagtgcaccg caggcgttgg catattgtaa tgcccgatgg gcgtcaaacc ctagctggcg
    18781 gcaggcaatc cacgcgccac caaagcagtc tcctgcaccg gtcgggtcaa cttcttccac
    18841 cggatagctc tcaacatgaa actgctcatt cgcggagtag taactggctc cttgattgcc
    18901 gcgcttcaca atcacctctt taacaccttc ttcaagaaaa ccagcgattg cacgctccgg
    18961 cgtcgagtgt ggcgacaaga gcaacacttc cccctcactg ggcatataga tatccgttaa
    19021 ctcaagaaca aagtggaggg catcgcgcat ttctggaata tcaagcattt ctttacgaat
    19081 attcgggtcg aaagaaataa cgccaccatt tgctttaacg atggttactg ctttttttac
    19141 tgcgtctacc atatggaatg aaaacaggga tgaacccata atatgaaaat gggtacaatc
    19201 cttcagaata ttttcatcaa catgctgtgc cgataattta ccgcaagcgg cattcttgat
    19261 attaaaaata aaatcgcgat cgccagagtt atgataagta acaaaggcac taccggtggc
    19321 ttccagaggt aagactgaaa tccccctgat gtccacaccg tcagcagcca gacgatgtat
    19381 attaatatca ccaaagccat cattccccac gcagctaatg atgccgcacg gaacgccaag
    19441 acgtgtcacc tggtcgataa aaatagcggg cgcaccactg ggataagggc cgttccagat
    19501 acctggttga tcaaatggct ggccgatttt actcgccata atttcaacca gaatttctcc
    19561 aattgtgaag accttactca tatgattaat tccttgaaat gattagctgc gttttctttt
    19621 atttcttacg tcaaaataaa cggctacaag aatgactaat cctttaacca ccatctggta
    19681 aaaataagga acattcatta agttcatgcc attattaata acgccgatgg ttaatgcgcc
    19741 gatcaccgtc cccaccacgg ttccatagcc gccgttcagg ctggtgcctc ccagtacagc
    19801 tgcggcaata gcatccagtt cgtagctcaa cgcggaattc ggttgcccgg aatagaggcg
    19861 tgaggcaagg atcatcccgc tgatacctgc cagcacggct gtaatcataa agactttaat
    19921 tttcaagcgg gtgacattta tccctgaata aattgccgct tctttattac caccagtgat
    19981 ataaattttt cgtccaaagg ttgttttagt aagaatgaag tggtttatca gcaataagat
    20041 gattaatatc catataggaa taggtatata gagaaattct ccattcccca gcgccagaaa
    20101 ggcatcatct ttaatcatga tgggcatacc atcggtcact atataagcga taccacggaa
    20161 gatccccata gtagcaacgg tgacaataaa cgaagggatc cccgctatag ccgtcaagcc
    20221 accatttatc atccccagag ccagtgctgc aataatggat aatgggacca ataaaacaaa
    20281 tggaattccc catgaagccc caagcgcagc aaatgtgcca atcaatgcaa tggttgaacc
    20341 gaccgataaa tcaatatcgc caagcaaaag cacataagtc attccatagg cggtaattgc
    20401 aattatcgaa acctgaagaa taatattagt aatgttattg gcggtaaaaa aactgtcatt
    20461 aataaaactg aacagaatga ttaacaccac cattccactg aggataccac catattgcct
    20521 gaagactttt ttaaaattat ttgcaatggg aattcccgaa ggtttcggac tggcaagagt
    20581 tttctctgtc atttaacgta ctcctgttgc agcggtcatt acctgtgtct gcgttaaatt
    20641 ttcacatgaa taaatatcgg caatagtttt attacgcatt accaccagac gatcgctaag
    20701 tgccatgact tctggcagtt cagaggacac cagaataatt gctgtccctg ccgcagccag
    20761 ttgacgtatc acgttataga tttcaaattt tgcgccgaca tccactccgc gcgttggctc
    20821 atcgagcaat aaaatacgcg gagttttctc cagccacttc gataacacga ttttctgttg
    20881 attaccgccg ctcagtgctc ccactgcctg atgcacagaa ctcactttag tgttcattct
    20941 ggcaattgat tgcagcgtct tcgcctcttc ttgtcggtga ttgatgagtc ccatccgcga
    21001 agcgttatat tgaaacgcca catggtgcgt gttcatattg acgtcgtgca tcagcactaa
    21061 cccttcttcc ttgcggtttt cggtcacaaa agcaatgcca tgatcaatag cctgtttggg
    21121 attagcgatc ctgacttcct taccatcaat caacacagtc ccgtgatacg aaacgaggcc
    21181 aaatagcgcc ttcatcacat cggaacgccc ggcccccacc aggccaaaaa atccgaggac
    21241 ttcccccgga cgcacagcga aactgacctc tttaaaaagc gaaggatggg aaagattttt
    21301 tacttccagt tttgcaggaa cattttcatc cggtttttcc ccaagacgtg ctggccaaat
    21361 atttttcatt tcccgaccaa ccatttgggc aatcaagtgc tggatatcac tgtttttctt
    21421 ttcatgcgta gcgatatact caccgtcgcg gatcaccgaa atatcatctg aaatccgcat
    21481 aatttcatcc atgcgatgag agatataaat aaccgcacag cctcgctgct ttaatttttc
    21541 aataatgtta aacaggatct ccgcttcatt atcacttagc gaagatgtcg gttcatccat
    21601 aatcacgaca tccgctgcat agcttaaggc tttagcaatt tctaccagtt gcctatgtgc
    21661 catcgacata ttgccgacca tttccagagg gtctagtggg atgcccagtt ccgcgaggag
    21721 gttctggcaa tctgcgagca tttttttatc gttaatgatg ccaaaacgtc gcggttcatt
    21781 attggcataa atattttccg ccacggtcat attattactc aggctcagtt cctgaaaaat
    21841 aatcgcaatc cctaactggc gagaatgctg cggattatta atggtgatgg tttcaccacg
    21901 tagtgttatc gttccctggg ttggcggata aacaccggcc agtattttca ttaatgtcga
    21961 tttaccggca ccattctcgc ctaataaagt atgcacccgc cctttacgta aagagagtgt
    22021 aatatctttt aatgcctgaa catgaccaaa tgtcttcgca atatgcgaaa catcaagaat
    22081 aacatccgtc ataatgacct cggtgtcaca acaaaacgcc aataacgttt tgttgtgagg
    22141 tgaagatatt tactatttac ctgtataaac gccgggttgt accgggatca ttttttctac
    22201 cggtttacca ttgaggtgat caacagcggc atcaatggct ttagcaccca tttgatctgg
    22261 atattgccga atcacggccg caaacgtttt ttcactgtca acggcatcac gcgcttcttt
    22321 catgccgtca aaaccaatga ttttgatgtt gtcgttatgc gcagattttg ctgcgataac
    22381 cgctgccagc gccgcatcat caccaaagcc aaacagacca ttcaggttag catttgcctg
    22441 cataatgttt tgcgcagtcg ttaacgcttc agctcgcgta ataccaggct gttcagcaac
    22501 aattttaata tccggtgtat cgcttaaccc tttcttaaaa ccatcaacgc gcgcaaccac
    22561 cgattgtagt gccggataag aaataatggc tacagtacct ttaccactca aaagtttacc
    22621 cattaacttg cctgcttcaa cgcctcccgc atagttatcg gtcgcaacat gcgatacgac
    22681 cggaacgcct tccgcagcga cgtcaacggt gataacggga ataccggcct tatcggcttt
    22741 aattatcgct gcctggacgc ctttcgaatc gaccggagag ataataatgg catccacttt
    22801 tttagtaata aaatcctcaa catcagaaag ttgtttgttt aaatcctgat tggcaataga
    22861 gacacttaaa ttgacattgt ctttttttgc ctcttctttc atggcattcg caagatcaat
    22921 atagaaagga tgttgttgtg ttaataatga cgcaccaatt ttatactctt ttgccaacga
    22981 actttgcgag aacaatgcca ggctcaccaa catagctgaa ccaagtaatt taatattttt
    23041 cataaacaat cctcattatt tttgtagggt acaacgtctt aaatttttcc ttcactacca
    23101 caaacgccaa taatacgagt tacgacttct ttcatggcgg cctttgcggg ctgcatatag
    23161 tgacgtggat catttgccgc aggattgttg tagaaatatc cttttaacgc atcggcaaac
    23221 gcaattttca attctgtcgc gacattcact ttacaaatac cgcgacgaat acagtgctga
    23281 acgtcctgtt ccgataagcc cgaagcacca tgcaacacca gcggaatctc cactcgctga
    23341 cgaatctgtt ccagccgcac aaaatccagt cgtggttcat gcgcatataa accatgtgct
    23401 gtgccgattg ctaccgccag ggagtcaatt tgggtacgtt gaacaaactc tgtagctgcg
    23461 gctggcgagg tataagcgga atctttttca tccacccgca gatcgtcctc ctggccgcca
    23521 agacgcccta gctcggcctc aacgctaaca tcaaagcgat ggcaaaaggt ggtaacttct
    23581 tgcactcgcg caatgttgtc ttcaaatggc aaatgggagc catcaatcat gacagaacgg
    23641 atccctgcgc gaaccttgtt aaaaatgtca tcactctctt catgatggtc gagatgcagg
    23701 actaacggca ggttgtactc gcgagccagc gattgtacta acgaaattaa acttcccacg
    23761 ccaccataac gatatgtacc gggtgttccc gccagcataa cgggagagcg aagttgtgca
    23821 gcgctctcaa caactacctg taaagtttca aggttatgaa tgttgaaagc agggacggca
    23881 tatccctctg actgggcttt tttcagcatg tttttgcttg aaataatgta catcgcagct
    23941 cctgttattt gtcgttcgcc ttacaaaatg aagaaagaga atcttcactt tcattttgaa
    24001 agcaaactag cacagcaaaa gcagcaaaaa gcgtgatgca tgtcgaacat catcaaaata
    24061 acttacaaaa aataacttaa tttatttttc actttgcaag tgaacc
//
LOCUS       DAJOTB010000069        23982 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJOTB010000069 DAJOTB010000000
VERSION     DAJOTB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.069"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            287..967
                     /locus_tag="OHN28_002637"
     CDS             287..967
                     /locus_tag="OHN28_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2298016.1"
                     /translation="MSMKTLLNVLTIFSLGTLTGCDYIEKASLIDDLAKQQELQKSKI
                     EELEKQQEQYKNKAELFEKQQNNITNSTRILASIVKTVKEKQDEFVFTEFNPLQTQYF
                     ILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNEKPTDAKALA
                     KWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPAE
                     TRQRKNKK"
     gene            1011..5354
                     /locus_tag="OHN28_002638"
     CDS             1011..5354
                     /locus_tag="OHN28_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCQ2298017.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            5772..7427
                     /gene="lldP"
                     /locus_tag="OHN28_002639"
     CDS             5772..7427
                     /gene="lldP"
                     /locus_tag="OHN28_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCQ2298018.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7427..8203
                     /gene="lldR"
                     /locus_tag="OHN28_002640"
     CDS             7427..8203
                     /gene="lldR"
                     /locus_tag="OHN28_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCQ2298019.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            8200..9390
                     /gene="lldD"
                     /locus_tag="OHN28_002641"
     CDS             8200..9390
                     /gene="lldD"
                     /locus_tag="OHN28_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCQ2298020.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9438..9911
                     /gene="trmL"
                     /locus_tag="OHN28_002642"
     CDS             9438..9911
                     /gene="trmL"
                     /locus_tag="OHN28_002642"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCQ2298021.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(9964..10785)
                     /gene="cysE"
                     /locus_tag="OHN28_002643"
     CDS             complement(9964..10785)
                     /gene="cysE"
                     /locus_tag="OHN28_002643"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCQ2298022.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10865..11884)
                     /gene="gpsA"
                     /locus_tag="OHN28_002644"
     CDS             complement(10865..11884)
                     /gene="gpsA"
                     /locus_tag="OHN28_002644"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCQ2298023.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11884..12351)
                     /gene="secB"
                     /locus_tag="OHN28_002645"
     CDS             complement(11884..12351)
                     /gene="secB"
                     /locus_tag="OHN28_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCQ2298024.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12415..12666)
                     /gene="grxC"
                     /locus_tag="OHN28_002646"
     CDS             complement(12415..12666)
                     /gene="grxC"
                     /locus_tag="OHN28_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCQ2298025.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12807..13238)
                     /locus_tag="OHN28_002647"
     CDS             complement(12807..13238)
                     /locus_tag="OHN28_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCQ2298026.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13482..15026
                     /gene="gpmM"
                     /locus_tag="OHN28_002648"
                     /gene_synonym="pgmI"
     CDS             13482..15026
                     /gene="gpmM"
                     /locus_tag="OHN28_002648"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2298027.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15036..16319
                     /gene="envC"
                     /locus_tag="OHN28_002649"
     CDS             15036..16319
                     /gene="envC"
                     /locus_tag="OHN28_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCQ2298028.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16323..17282
                     /locus_tag="OHN28_002650"
     CDS             16323..17282
                     /locus_tag="OHN28_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCQ2298029.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17269..18303)
                     /gene="waaH"
                     /locus_tag="OHN28_002651"
     CDS             complement(17269..18303)
                     /gene="waaH"
                     /locus_tag="OHN28_002651"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCQ2298030.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18542..19567)
                     /gene="tdh"
                     /locus_tag="OHN28_002652"
     CDS             complement(18542..19567)
                     /gene="tdh"
                     /locus_tag="OHN28_002652"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCQ2298031.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19577..20773)
                     /gene="kbl"
                     /locus_tag="OHN28_002653"
     CDS             complement(19577..20773)
                     /gene="kbl"
                     /locus_tag="OHN28_002653"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCQ2298032.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            20987..21919
                     /gene="rfaD"
                     /locus_tag="OHN28_002654"
     CDS             20987..21919
                     /gene="rfaD"
                     /locus_tag="OHN28_002654"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCQ2298033.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            21929..22975
                     /gene="rfaF"
                     /locus_tag="OHN28_002655"
     CDS             21929..22975
                     /gene="rfaF"
                     /locus_tag="OHN28_002655"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCQ2298034.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            22979..23938
                     /gene="rfaC"
                     /locus_tag="OHN28_002656"
     CDS             22979..23938
                     /gene="rfaC"
                     /locus_tag="OHN28_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCQ2298035.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
BASE COUNT         6033 a         6204 c         6172 g         5573 t
ORIGIN      
        1 ttcatcacaa cacatcattt atatgaatat aaaatctccc tgcgctcccc agcctaagac
       61 actaaacatt tgacattgat tgataccgct cattaaaatt cgcgcgactc ataacattcg
      121 ctacaaactg aaatcagttg tcatttaata aaacgtccgt aataccaatt atttcaataa
      181 atctcttata agttttattg cgaatacata tataaaaaac attcatcaga agaatgaata
      241 tggcgtaatt atcaacatat tggggttatg aaaaggaatt catcctatga gcatgaaaac
      301 acttttaaac gtgttaacaa tattttcact aggtacatta acaggctgtg attatattga
      361 aaaagcgagt ctgatcgatg atcttgccaa acagcaagag ttgcaaaaaa gtaaaataga
      421 ggagcttgag aaacaacaag aacaatataa aaataaagca gaactgtttg aaaaacaaca
      481 aaataatatc acaaacagca ccagaatact tgccagcatt gtaaagacag tcaaagaaaa
      541 acaggacgaa tttgtcttca ccgaatttaa cccgctacaa acccaatact tcattttaaa
      601 taacggctcg gtcggtttgg caggccgggt gctggctatt gatgcggttg aaaatggcag
      661 tgtcattcgt atttcgctgg ttaacttatt aagcgttccc gtatcaaata ttggctttca
      721 tgcgacatgg gggaatgaga aacccaccga tgccaaagct ctggcgaaat ggcaacaatt
      781 actattcaac accaccctga attccactct gcaattaatg cctggtcaat ggcaagacat
      841 taatctgaca ttaaaaggcg tctcgcctaa taacctgaag tatctgaagt tgtccatcaa
      901 tatggctaat cttcagttcg acactgttca acctgctgaa acccggcagc ggaaaaacaa
      961 aaaataaatg cagcttctgc gcttatatca aggaatagag agcatcaata atgaacaaaa
     1021 tatttaaagt tatctggaat ccggcgacag gcagttacaa cgtcgccagc gaaacggcga
     1081 agagccgtgg caaaaagagc gggcgcagta agctgttaat ttctgccttg gttgcaggtg
     1141 ggctgttatc gtcatttggg gcactggctg ataactacga ggggcagcca gtggattatg
     1201 gcaatggctc agcaggtgac ggctgggttg ctatcggtaa aaattcaaaa gcaaatacct
     1261 ttgtcaatgg ctctggtgcg agcactgcgc taggttatga cgcgatagct gaaggtgagt
     1321 acagctcagc tataggctca cgaactcatg cgattggtgg tgcatcaatg gcctttgggg
     1381 ttagtgcaca agcaataggt gacagaagtg tcgcgctggg tgcatcttca gtagcaactg
     1441 gcggtcgttc gatggctctt ggtcgttacg caaagacgaa tggttttaca tctcttgcta
     1501 ttggggacac cgcccttgcc gatggtgaaa aaactattgc gttaggaaat acggctcaag
     1561 cttacgaaat tatgagcatc gccctcggtg atcatgctaa tgcctcaaaa gaatattcaa
     1621 tggcgctggg agcaagtagc gttgcatctg cggctaacgc cattgccgta ggccgcgaca
     1681 gtgctgctaa cggcacagac agccttgcct taggcagact atctctcgcc agcgcagcaa
     1741 acgcgattgc tatgggtgca gagagcgaag ctgctgaaaa tgcgacggcg attggcttta
     1801 acgcggatgc gatcgggaag tccagcctgg cgctgggaga caacgccagc gctggtgaaa
     1861 ccaactccat cgctataggt caaggcagcg aggcaagcaa acttgattca attgcgctgg
     1921 gaagtaacag caggtcagcg ggtgagaacg ccattgcact gggcaacaac agtaatgcgg
     1981 ggggcaaaaa cagccttgct ttcggtttta acaccaccgc aaatggtgat aacgcagtcg
     2041 ccattggtgc aaattcttcc gcaggtgctg acaataccgt gtctgtaggt agctctagtt
     2101 taaagcgcaa aattgtcaat atgggtaatg gtgacatcaa taatgtcagc tccgatgcca
     2161 tcaatggatc acaactttat gccatcagta agtctgtatc cgacaggctt ggaggatatc
     2221 atgatgatcc tgataatgtt ataaactctg acgggacgct taaagctccc acatattatc
     2281 tacaaagtgg ccaatacaat aacgtgggag aggctctcca gagcatcgac aataacacgt
     2341 tgcactggga cagcaaaagt aataaataca gtgccagcca tactgtcttt aatgcaaatg
     2401 gctcagttaa aagcacctcg gcgaaaaaca tcattaccga cgtcgctgat ggcacgattt
     2461 cagccaccag ttccgacgcc gttaacggtt cgcagttgta taatctgaaa caagatgcct
     2521 tgctatggga tggcactgcg tttagcgcca aacatggcac cagcaatact aacagcaaaa
     2581 tcaccaacgt tgcggatggt gctgtttcag cctccagtaa agatgccgtt aacggttcgc
     2641 agttgtatga tctgaaacaa gatgccctgc tatgggatgg cactgcgttt agcgccaaac
     2701 atggcaccag caatactgac agcaaaatca ccaacgtact ggcaggcaca gtatcttcca
     2761 ccagcaccga tgccattaat ggtacacaac ttcaaagcac tgcaagcacc attacgtcct
     2821 atttgggcgg cggcgcggca atgagtaatg acggcgtatt taccggtcct caatataata
     2881 ttggtggtaa gaattacacc aatgttggcg cagctcttac cgctctggac aatgacgccc
     2941 tgttgtggga tgcaaccgct aattcaaata ctggcgcatt cagtgctgca cacggaacac
     3001 tcaaaaccgc cagcaagatt accaacgttg caaatggtga catattagcc aacagttatg
     3061 atgctattaa tggctcacaa ctttatagtt tagctgattc atttacgtca taccttggcg
     3121 gtggtgctga tatcaacaat gcgggtgttc tcatagcccc gacctatact atcggttcga
     3181 aaacgtacaa caacgtaggt gatgccctta acgcgattaa ctcatcattc agcacctcaa
     3241 tcagtgatgc cctgctgtgg gattctacag cgaacaaatt cagcgccaaa cacggtggta
     3301 ccaatagcat tattactaac gttgcagacg gtacaatttc ctctaccagc agcgatgctg
     3361 taaatggctc gcaactctat gacacgagca agtacattgc tgatgccctg ggtggtgacg
     3421 cagaagttaa cgctgacggc accatgactg caccgactta ctctatcgct aatgaagatt
     3481 accacagcgt aggtgaagcc ctggatgcgc tcgatgataa cgcgctgctg tgggatgaag
     3541 acgcaggtgc ctacaacgcc agccatgatg gcaatgccag caaaattacc aacgttgcgg
     3601 caggtgatct ctccacaacc agtaccgatg ctgttaacgg ttcccagtta aacgcaacca
     3661 atattctggt tacgcaaaat agccaaatga ttaaccagct tgccggtaac accagcgaaa
     3721 cctacatcga ggaaaacggt gcgggtatta actatgtacg taccaacgac aacggcttag
     3781 cgttcaacga tgccagcgcc tcaggtattg gtgctacggc tgtgggttat aactctgttg
     3841 ccgaaggtga tagcagcgtg gccattggtc aaggtagcag cagctccgtt gataccggca
     3901 ttgccttggg tagcagctcc gtttccagtc gcgtgattgt taaaggttct cgtaacacta
     3961 gtgtaacgga aaatggcgta gttatcggtt atgacaccac tgacggtgag ttgctcggtg
     4021 cattgtcaat tggtgatgac ggtaaatacc gtcaaatcat caacgtcgcc gatggttccg
     4081 aagcccatga cgccgttacg gttcgccagc tgcaaaatgc cattggtgca gtcgctacca
     4141 cgccgactaa atacttccac gctaactcaa cggctgaaga ctcactggca gtcggtgaag
     4201 actcgctggc aatgggcgcg aaaaccatcg ttaatggtaa tgcgggtatt ggtatcggcc
     4261 tgaacacgct ggttctggct gatgcgatca acggtattgc tatcggttct aacgcacgcg
     4321 caaatcatgc cgacagcatt gcaatgggta atggttctca gactacccgt ggtgcgcaga
     4381 ccaactacac tgcctacaac atggacgcgc cgcagaactc cgtgggtgag ttctctgtcg
     4441 gcagtgaaga cggtcaacgt cagatcacca acgtcgcagc ggggtcggcg gataccgatg
     4501 cggtcaacgt gggtcaattg aaagtaacgg acgcgcaggt ttcccagaat acccagagca
     4561 ttactaacct gaacactcag gtcactaatc tggatactcg cgtgaccaat atcgaaaacg
     4621 gcattggcga tatcgtaacc accggtagca ctaagtactt caagaccaac accgatggcg
     4681 cagatgccaa cgcgcagggt aaagacagtg ttgcgattgg ttctggttcc attgctgccg
     4741 ctgacaacag cgtcgcgctg ggcacgggtt ccgtagcaga cgaagaaaat acgatctctg
     4801 tgggttcttc taccaaccag cgccgtatca ccaacgttgc tgccggtgtt aatgccaccg
     4861 atgcggttaa cgtttcgcaa ctgaagtctt ctgaagcagg cggcgttcgc tacgacacca
     4921 aagctgatgg ctctatcgac tacagcaaca tcactctcgg tggtggtaac ggcggtacga
     4981 ctcgcatcag caacgtctcc gctggcgtca acaacaacga cgcagtgaac tatgcgcaac
     5041 tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca gcgcatggtt gagatggata
     5101 acaaactgtc caaaaccgaa agcaagttga gcggtggtat cgcttctgca atggcgatga
     5161 ccggtctgcc gcaggcttac acgccgggtg ccagcatggc ctctattggt ggcggtactt
     5221 acaacggtga atcggctgtt gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg
     5281 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     5341 gtattcagtg gtaatgatcc attaacgaat gggtaaacgc cacattgcct gatgcgctac
     5401 gcttatcagg cctacggggt gattcagtac attcctgtag gtcggataag gcgttcacgc
     5461 cgcatccggc aagataaggc actccgggtc aacaatctaa gggcaattct ctgatgagga
     5521 ttgccctttt ctttaccaga catctcccac cacaagaatt ggccctacca attcttcgct
     5581 tatctgacct ctggttcaca attccccaat taaaactcac atcaatgttg ccaacacgta
     5641 acatttagtt aaccattcat tgtcattacc cctacacaac acaattggca gtgccacttt
     5701 tacacaacgt gtgacaaaga gatgggcaac agactcatta cacggcgtgt gtggactgca
     5761 ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat atctggcttt
     5821 ccagcctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt aagctcaaac
     5881 tgaaaggata cgtcgccgcc tcatggacgg tggcaatcgc ccttgccgtg gctttgctgt
     5941 tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc ttctacgggt
     6001 tgtggcccat cgcgtggatc attattgcag cggtgttcgt ctataagatc tcggtgaaaa
     6061 ccgggcagtt tgacatcatc cgctcgtcta ttctttcgat aacccctgac cagcgcctgc
     6121 aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca ggctttggcg
     6181 caccggtagc aattaccgcc gcattgctgg tcggcctggg ctttaaaccg ctgtacgccg
     6241 ccgggctgtg cctgattgtt aacaccgcgc cagtggcatt tggtgcgatg ggtattccga
     6301 ttctggtcgc cgggcaggta acaggcatcg acagctttga gattggtcag atggtggggc
     6361 gtcagctgcc gtttatgacc attatcgtgc tgttctggat catggcgatt atggacggct
     6421 ggcgcggtat caaagaaacc tggcctgcgg tcgtggttgc gggcgggtcg tttgccatcg
     6481 ctcaatacct cagctctaac tttattgggc cggaactgcc agacattatc tcttcgctgg
     6541 tatcgctgct ctgtctgaca ctgttcctca aacgctggca acccgtgcgc gtattccgct
     6601 tcggtgactt aggggcgtca caggttgata tgacgctggc tcacacaggt tacacggcag
     6661 gtcaggtgct gcgcgcctgg acaccgttcc tgttcctgac cgccaccgta acactgtgga
     6721 gtatcccgcc gtttaaagcc ctgttcactt caggcggtgc gctgtatgag tgggtgatca
     6781 acattccggt gccgtacctc gataaactgg ttgcccgtat gccgccagtg gtcagcgaag
     6841 cgacagcgta tgccgccgtg tttaagttcg actggttctc tgccaccggt accgccattc
     6901 tgtttgctgc cctgctctcg attgtctggc tgaagatgaa accatctgac gctatcagca
     6961 ccttcggcag cacgctgaaa gaactggctc tgcctatcta ctccatcggc atggtgctgg
     7021 cgttcgcctt tatctcgaac tattccggtt tgtcatcaac actggcgctg gcactggcgc
     7081 acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggcttggg gtattcctga
     7141 ccgggtcgga tacgtcatct aatgctttgt ttgccgcttt gcaggctacc gccgcacaac
     7201 aaattggcgt ttctgacctg ttgctggttg ctgccaacac caccggtggt gtcaccggca
     7261 aaatgatctc tccgcaatct atcgctatcg cctgtgcggc ggtaggcctg gtgggcaaag
     7321 agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt atggtgggcg
     7381 tgatcaccac gcttcaggcc tatgtcttaa cgtggatgat tccttaatga ttgttttacc
     7441 cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg aaaaaaacct
     7501 ggaagcgggc atgaagttgc ccgctgaacg tcaactggcg atgcaactcg gcgtgtcgag
     7561 aaattcactg cgcgaggcgc tggcgaaact ggtaagcgaa ggtgtgctgc tcagtcgacg
     7621 tggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa acatcgtcca
     7681 gccgctgaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc tggaagcccg
     7741 ctacgccatt gaagccagca ccgcatggca tgcagcaatg cgcgccacac ctggcgaaaa
     7801 agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggatc tcgcctcaca
     7861 agcggacgtt cgttttcatc tggcgattgc cgaagcttca cataacattg tgttgctgca
     7921 aaccatgcgc ggtttcttcg atgtcctgca atcctctgtg aagcatagcc gccagcggat
     7981 gtacctggta cccccggttt tttctcagct gaccgaacaa catcaggcgg tcattgacgc
     8041 catttttgtc ggtgatgctg acggggcgcg taaagcaatg atggcgcacc ttagctttgt
     8101 tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc catgcacgaa ttacccgcct
     8161 gcctggtgac cataatgagc attcgaggga gaaaaacgca tgattatttc cgcagccagc
     8221 gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgtttcacta tatggatggg
     8281 ggggcatatt ctgaatacac cctacgccgc aacgtggaag atttgtcaga agtggcgctg
     8341 cgccagcgta ttctgaaaaa catgtctgac ttaagcctgg aaacgacgct gtttaatgag
     8401 aaattgtcga tgccggtggc gctaggtccg gtaggtttgt gtggcatgta tgcgcgacgc
     8461 ggcgaagttc aggctgccaa agccgcggat gcgcatggca ttccgtttac tctctcgacg
     8521 gtttccgttt gcccgattga agaagtcgct ccagctatca aacgtccgat gtggttccag
     8581 ctttatgtgc tgcgcgatcg cggctttatg cgtaacgcac tggagcgagc aaaagccgcg
     8641 ggttgttcga cgctggtttt caccgtggat atgccgacac cgggagcgcg ttatcgtgat
     8701 gcgcattctg ggatgagcgg cccgaacgcg gcaatgcgcc gctatttgca ggcggtgacg
     8761 catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cgcatgattt aggtaatatc
     8821 tcggcttacc tcggcaaacc gaccggactg gaagattaca tcggctggct ggggaataac
     8881 ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg ggatggcccg
     8941 atggtgatca aagggatcct cgacccagaa gatgcgcgcg atgcagtacg ttttggtgct
     9001 gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtgct ctcttctgcc
     9061 cgtgcactgc ctgctattgc agatgccgtg aaaggtgata tcgccattct ggcagatagc
     9121 ggaatacgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga caccgtactg
     9181 ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt agctaacctg
     9241 ctaaatctga tcgaaaaaga gatgaaagtg gcaatgacgc tgactggcgc gaaatcgatt
     9301 agcgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc ggcggcattg
     9361 gctccgatgg cgaaagggaa tgcagcttaa gggttagacg aatatctgct atcctgcccc
     9421 cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata
     9481 ctggcaacat catccgtctt tgcgctaata ccggctttcg tctgcatatc atcgaaccga
     9541 tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg gctggactat cacgagttta
     9601 ccgccgttac gcgtcatcat gactatcgcg cgtttctcga agcagaaaat ccccagcgtc
     9661 tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg
     9721 attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc
     9781 ccgctgaaca aaaaattcgt attccaatgg tgccggacag ccgcagcatg aacctgtcca
     9841 atgcggtgtc ggtcgtggtg tatgaagcct ggcggcagtt ggggtatccg ggagcggtat
     9901 tgagagatta gttactgtag gccggataga tgattacatc gcatccggca cgatcacagg
     9961 acatcagatc ccatcaccat actcaaacgt atggttaatg ccgttgaaat gctgatccat
    10021 atccattgac ggtttatcgc tatccggctt gccgacaatg cgtgccggaa cgccagcggc
    10081 agtagtgtgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg
    10141 cccaacttca atattgccga gaattttcgc gcccgcgcca atcatcacac cttcacgaat
    10201 tttcgggtga cggtcgccac cagatttacc cgtaccgcca agcgtcacag attgcagaat
    10261 cgatacgtcg ttttcaatca ccgccgtttc accaacgacg atgcctgtcg cgtggtcgag
    10321 catgataccg cgaccaattt ttgctgccgg gtgaatatca acctggaacg tcacagaaac
    10381 ctggttttgc agaaaaatag ccagtgcgcg acgcccctga ttccacaacc agtgaccgat
    10441 gcgatacgcc tgcaaagcat gaaaaccctt caggtatagc aacggggttg agtatttatc
    10501 caccgccggg tcacgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc
    10561 cgggtcggcg gcgtaggctt cttccaccac ttcacggata gcaatagccg gcataatggg
    10621 cgatgacagc ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt cgtgcttgag
    10681 tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc
    10741 agctttaata ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg
    10801 atgagataat gaccgacccg ttctgcgcgg gccgggtcat agtggtaaca aaggttccct
    10861 gggtttagtg gctgctgcgc tcgtccttgc gtgcacgacc tagtaaagtc aatgctgcct
    10921 cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa
    10981 cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccactt
    11041 gaccaatttt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc atgccaaaac
    11101 ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc
    11161 ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag acgcgacatc tcagccagcc
    11221 cacgagtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtcg gacatccccg
    11281 cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacaccgatg aaatccgggt
    11341 tgctgtaaac gcggaaactt ttgccacagt gcagcaattg ctggaggtca tcggcaaagg
    11401 tctggtccgt cgaggccagc gaaatggccg tcggtaaacc tgccgccagt tctttcgcaa
    11461 acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt
    11521 cctgtaacag acgtccggtt tctgcttcca gccctttggt tgcccacacc agacgcgcat
    11581 caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga
    11641 cgacgagaat attacggctg gctgccagcg cagtggcgag atcgctttca agatggagcg
    11701 tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg
    11761 cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtga
    11821 tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt
    11881 tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat
    11941 gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac
    12001 catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag
    12061 gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac
    12121 ttcacacagg aacgcggttt cttcgcccag ggaggccgtt acggtaacac gcagtaccac
    12181 ttcgtaaacg tcatctgcca gttgagtaga tgccgtatcc agatcaagtt taacttctgg
    12241 ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaaa tatccttggt
    12301 ataaatacgt tggatctgga aagtcatttc ggtgttgttt tgttctgaca tgtgtagaaa
    12361 acccttaagt gttgtcctta aaatactgcg caatgtaatg ccaacgttca cacgttattt
    12421 cagcagggga tccagtccac cacgtgcatc caatgcatac aagtcatcac agccaccaat
    12481 gtgctgtgcg tcaataaaaa tctgtggaac cgtggtgcga ccgctgcgtt tgatcatctc
    12541 ttcacgctta gcggcattgc catcgatcgg caactcctga aaactcacgc ccttgctgct
    12601 tagcagtgct tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt
    12661 ggccataaat tagctcctgt tttttaccct gaaatttcat gttgcagggc ggcagcaaaa
    12721 tggagtgtcc ccagaagctt atatcaataa gtcgctgggg gcctaattgc agctaacggc
    12781 ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccaaaggcaa gttttcgcca
    12841 gcccagccag cgacgccttc tttcagtacg aatacttgtg caaaaccagc tttcgtcagc
    12901 gcgtttgcag gctcctggca ctgcatgcca gaaccatcta ccacgataac cggtttgtct
    12961 ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa caggttgata
    13021 gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg
    13081 tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc
    13141 aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt
    13201 atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt
    13261 agtgattaaa aaaccaggaa aggagtatac ctgcgcagtg cggcaaatac agccagcgcg
    13321 ttaactggaa tgcaattttg cggggcgcta cgaaaaaaaa gcgtaaatct ggcatctgct
    13381 tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    13441 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    13501 ctatggtact ggtgattctg gatggctatg gctatcgcga agaacagcag gataacgcca
    13561 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    13621 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    13681 gccacgttaa cctgggtgca ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    13741 aaatcaaaga tcgcactttc tttgctaatc cggtgttgac tggtgcggta gataaagcga
    13801 aaaacgcagg caaagcagta cacattatgg gtctgctctc cgcaggtggt gtacacagcc
    13861 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    13921 acctacacgc attccttgac ggtcgcgaca ctccgccgcg cagtgctgaa tcctcgctga
    13981 aaaaattcga agagaaattt gccgcgctgg gtaaaggccg cgtagcgtcc atcattggtc
    14041 gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagct tatgacctgc
    14101 tgactctggc gcaaggtgag ttccaggccg ataccgccgt tgccggtttg caggctgctt
    14161 atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc
    14221 cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg
    14281 cacgtgaaat cactcgtgca ttcgtaaatg ccgattttga cggcttcgcg cgtaagaaag
    14341 tggttaacgt cgatttcgtg atgctgaccg aatacgctgc cgacatcaaa accgcagtgg
    14401 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    14461 ctcagctgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    14521 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    14581 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcaa
    14641 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    14701 acaccggggt gatggaagcg gcggttaaag cggttgaagc gctggatcac tgcgtggaag
    14761 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    14821 acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacagcacac accaacctgc
    14881 cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgaaggc ggcaaacttt
    14941 ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    15001 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    15061 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    15121 tgagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    15181 aatccattca ggctgacatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac
    15241 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta
    15301 aactgcgcga aacgcaaaac acgcttaatc aactgaataa acagattgat gagatgaacg
    15361 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcgcaac
    15421 tggatgccgc gtttcgtcag ggtgaacata ccggtattca gctgattctc agcggtgaag
    15481 aaagccagcg tgggcagcgg ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    15541 aaaccattgc tcagttgaaa caaacgcgtg aagaagtcgc tatgcagcgt gccgaactgg
    15601 aagagaaaca aagcgagcaa caaacgcttt tatatgagca gcgcgcccaa caggcgaagc
    15661 tgacccaggc gttgagcgag cgtaaaaaga cgctggcagg gctggagtct tccatccagc
    15721 aaggtcagca acagttgagc gagctgcgcg ccaacgaatc ccgcctgcgt aacagcattg
    15781 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgtga agcgcgcgaa gcccaggcgg
    15841 ttcgcgaccg ccagaaagaa gcgacgcgca aaggcaccac ctacaagccg accgaaagcg
    15901 aaaaatcgct gatgtcccgt accggtggtc tgggcgcacc gcgcggtcag gcattctggc
    15961 cggttcgcgg gccaacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    16021 aagggatggt gattggtgcc tctgaaggta ctgaagttaa agcgattgcc gacggccggg
    16081 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag catggtaaag
    16141 gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc
    16201 gcgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct
    16261 atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag
    16321 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    16381 ccgtaattgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg
    16441 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    16501 acgccagaga gatggcgacc aaagcacata acagcggtca cgaagtgttg attcatctac
    16561 cgatggcacc actgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca
    16621 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    16681 tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    16741 aggcgctgga gcgttacaat ctttactttc tcgacagcgt aaccatcggt aatacccagg
    16801 cgatgcgcgc cgcgcaaggc actggcgtga aggtgatcaa acggaaggtg ttcctcgacg
    16861 attcgcagaa cgaagcggac atccgtgtgc aattcaatcg cgcaattgac ctagcgcgtc
    16921 gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac
    16981 agctggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    17041 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    17101 cgttccgtgg cgtgaagctg tgcaaaccga agaagccgct ggaacctgtt tatgctaatc
    17161 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc
    17221 atcagtggca aggctggggc aaacagcccg aagcggcaaa acttaacgct agcgcaaatt
    17281 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    17341 gaaagaccac agtaacgcct ggtaacccac cttcacgctg cggacattgg taatcaggcg
    17401 cttatacata ccggaagtaa atatctcggc aatcatccgt tggcgggtaa gaatatccgg
    17461 ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc
    17521 cggataaatc gtaattttgt cagcataatt tcgatttaat ttctccagca ggcgggtaat
    17581 cttaatatag tgacgttgat aattaaggtt tttattccct tgtctatgca accgactcac
    17641 tgacgtatta tgcagataat atttatataa tgattgctcg gtatatcgcg cacgcagcgc
    17701 gttaaacata aattctgttg tccagacaat atcctgatga tgtaatccgg caataaattt
    17761 aatgttattt ttaacaataa catcgcggcg ataaaccccc atccagacca cgtgtgtcca
    17821 acggcgggaa gaaagtccca tccgcagcca gtccggaccg gttaatacac cggttgagcg
    17881 aaggcgatcg gtggggatgg attgccaggt ttcacccgtt tcacgaaaac accaatcagc
    17941 gttgcactgc gccacgtcga ggtcatcctc taacgccatg gtcatcagcg tttcgtacat
    18001 ggtgggatag acctcatcgt cagcatcgac aaaagcgaca tattttcccg ttgccacttc
    18061 aatcccacga ttacgcgcca ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg
    18121 cggatagttt tctgcgtaat gctttgctat ttcaacagaa ttatccgttg aaccatcgtt
    18181 aataataatg atttccagag cagtccatgt ttgcgtaatt aaagattcca tacaagtgcg
    18241 gaaatcatcg cccgcattat ataacgggat aataacacta agtttattgg tgctgttcat
    18301 cataaattac cgacgacctc tgaagttatc ctgacgtttt acaggctttg tattaagaaa
    18361 ctattaagcc tgtgagatat tatttttacc ggtgagagag tcacttcagg gtagagagat
    18421 aatgagagca gctatataag agatcagtgc aattacgaac tctttattac actgacagga
    18481 ttttacatat ttaacggatt atcctcaggc aaaagggctg gaataccagc ccttgttcgt
    18541 gttaatccca gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct
    18601 tctggaaatc atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact
    18661 gaatcagcgc cgccatcttg taccaggttt caaacatctc acgaccgtaa atacctttaa
    18721 tgaacaagcc tttaaagatt actttggtcc agtcgataga catatcggac ggtggaatac
    18781 ccagcatcgc aatacgaccg ccgtggttca tggtgtcgag catggtacga aacgctggcg
    18841 gcgcaccgga catttccaga ccgacatcaa agccttcggt catgcccagt tctgccatca
    18901 catcattaag gttttcttta gcgacgttaa ccgcacgggt gatacccatt ttgcgtgcca
    18961 gctcaaggcg gtattcgtta acatcagtga tcaccacatt gcgtgcacca acgtgtttcg
    19021 ccaccgccgc agccataata cctatcggac ctgcaccaga gaccagcaca tcctcgccaa
    19081 ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca
    19141 ggtcgtcgga gatattgtct gggattttga aggcgttgaa cgccggaatc accagatact
    19201 cggcgaagca gcccggacgg ttaacaccga cgccgatcgt gttgcggcac agatgggtac
    19261 gaccgccacg acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat
    19321 cgccaatctt gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat
    19381 ggcccacgac catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt
    19441 gaacgtcagt cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga
    19501 gttccggtac aggaacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg
    19561 ctttcatctc acaccctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt
    19621 ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg
    19681 cctgaccttt aggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca
    19741 gttcacgggc aaatttttgc gccactacag catcgccaag catgaccgga ataatggcgt
    19801 gatcggcacc cgccagggta aagcccgccg ccgacatctg ctcacggaac tgacgcgcgt
    19861 tcgcccacag acggtcacga agctcactgc ccgcttcgac catctccagc actttaatgg
    19921 aagcagccac aatggcaggt gccagtgagt tggagaacag gtaaggacga gaacgctgac
    19981 gcagccactc aaccacttct ttacgcgctg cggtgtaacc accagaagcc ccgcccaacg
    20041 ctttaccaag cgtaccggtg ataatatcga cccgccccat cacatcgcag tattcatggg
    20101 aaccacgacc attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg
    20161 catcgtattt atctgccaga tcgcaaacgc ccttcaggtt ggcaatcacg ccgtccatcg
    20221 agaacacacc atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca
    20281 gacgagcttc cagctcatgc atatcgttgt tggcataacg atagcgttta gctttacaca
    20341 gacgcacacc atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcctccg
    20401 cgctcagaag cgtttcaaac aggccgccgt tagcatcaaa gcaggaagag tagagaatcg
    20461 catcttccat ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctatcctggg
    20521 tgccgcagat aaagcgcacc gaagccatcc caaaaccatg agagtccatt cccgcttttg
    20581 ccgccgcaat cagctctgga tgatttgcca gtccgagata gttattggcg caaaagttaa
    20641 taacgtggct tccatcagcc acagtgatat ctgcttgctg cgcagacgta ataatgcgct
    20701 cttctttaaa caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa
    20761 attctccacg cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt
    20821 ctaggtcgcg acgagatgac gcgttaacac ttctgcaaaa ttcaggataa ataacgtcca
    20881 tcaatctcac ttttggcgaa tggctgaagc caacgccgtg tagtgatatg atagaagtat
    20941 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    21001 cggcggcgcg ggctttatcg gcagcaacat cgttaaggcc ctgaatgata aaggcatcac
    21061 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    21121 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    21181 gttcggcgat gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga
    21241 cggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    21301 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acttacggtg gacgcacctc
    21361 cgactttatt gaatcccgcg aatatgaaaa accgttgaat gtctacggtt actcaaaatt
    21421 cctgtttgat gaatatgttc gccaaatcct gcctgaagcg aactcgcaga ttgttggctt
    21481 ccgttatttc aacgtttatg gacctcgtga aggccataaa ggcagtatgg cgagcgtcgc
    21541 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    21601 gaactttaaa cgtgacttcg tctatgtggg cgacgtggca gatgtaaacc tgtggttcct
    21661 ggaaaatggc gtttccggca tcttcaacct cggcaccggt cgtgcggaat ccttccaggc
    21721 agtagcagat gctacgcttg cttatcacaa gaaaggccaa atcgaataca ttccgttccc
    21781 ggataaactg aaaggccgct accaggcgtt cactcaggca gatctgacga atctgcgcgc
    21841 ggcgggttac gataaaccgt tcaaaaccgt tgccgaaggc gtaacggaat acatggcctg
    21901 gctgaatcgt gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    21961 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    22021 gataatcgac gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    22081 taacgaagct atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    22141 actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac ctaactcctt
    22201 caaatccgca ttagtgcctt tcttcgcagg tattcctcat cgcaccggct ggcgcggcga
    22261 gatgcgctac ggtttactca acgatgtacg cgtgctcgat aaagaagcct ggccgctaat
    22321 ggtggaacgc tatgtcgcgc tggcctatga caaaggcatt atgcgtacag cacaagatct
    22381 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    22441 taatcaattt tcgctctcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt
    22501 tggtccggca aaacgctggc cacactacca ctatgcggag ctggcaaagc agctgattga
    22561 tgaaggttat caggtggttc tgtttggctc ggcgaaagat catgaagcgg gcaatgagat
    22621 tcttgccgct ttgaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca
    22681 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgttacta acgattctgg
    22741 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccgagtag
    22801 cccggacttc acaccaccgc tatcccataa agcacgcgtg atccgcctga ttaccggcta
    22861 tcacaaagtg cgtaaaggtg acgcggcgga gggttatcac cagagcttga tcgacattac
    22921 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    22981 gcgggttctg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgc tgcccgcact
    23041 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt
    23101 cgcacagatt ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg
    23161 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    23221 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcgac
    23281 gctggtgacg cgtctggcgc atggcgtaaa gcatggaatg gactggcaaa ccgctcgcga
    23341 accgttagcc agcctgtttt acaatcgtaa acatcatatt gcaaaacagc agcacgccgt
    23401 agaacgcacc cgcgaactgt ttgccaaaag tctgggctat agcaaaccac aaacccaggg
    23461 cgattatgct atcgcacagc atttcctgac gaacctgcct acagatgctg gcgaatatgc
    23521 cgtatttctt catgcaacaa cccgagatga taaacactgg ccggaagaac actggcgaga
    23581 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    23641 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    23701 gatgagtctg gaaggcgttg cccgcgtact ggccggggct aaatttgtag tgtcggtgga
    23761 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    23821 taccgatccg ggattaattg gtgggtatgg gaagaatcag atggtttgta gggctccaag
    23881 agaaaattta attaacctca acagtcaagc agttttggaa aagttatcat cattataaag
    23941 gtaaaacatg ctaacatcct ttaaacttca ttcattgaaa cc
//
LOCUS       DAJOTB010000070        23697 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJOTB010000070 DAJOTB010000000
VERSION     DAJOTB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.070"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..326
                     /locus_tag="OHN28_002657"
     CDS             <1..326
                     /locus_tag="OHN28_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2298036.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(369..1520)
                     /locus_tag="OHN28_002658"
     CDS             complement(369..1520)
                     /locus_tag="OHN28_002658"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCQ2298037.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1534..3129)
                     /locus_tag="OHN28_002659"
     CDS             complement(1534..3129)
                     /locus_tag="OHN28_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2298038.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3276..4034)
                     /gene="aroD"
                     /locus_tag="OHN28_002660"
     CDS             complement(3276..4034)
                     /gene="aroD"
                     /locus_tag="OHN28_002660"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCQ2298039.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4065..4931)
                     /gene="ydiB"
                     /locus_tag="OHN28_002661"
     CDS             complement(4065..4931)
                     /gene="ydiB"
                     /locus_tag="OHN28_002661"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCQ2298040.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMEPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4943..6208)
                     /locus_tag="OHN28_002662"
     CDS             complement(4943..6208)
                     /locus_tag="OHN28_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298041.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6435..7649)
                     /locus_tag="OHN28_002663"
     CDS             complement(6435..7649)
                     /locus_tag="OHN28_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298042.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7749..8105)
                     /locus_tag="OHN28_002664"
     CDS             complement(7749..8105)
                     /locus_tag="OHN28_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCQ2298043.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8241..8348)
                     /gene="rprA"
                     /locus_tag="OHN28_002665"
     ncRNA           complement(8241..8348)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OHN28_002665"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8534..9646)
                     /gene="ydiK"
                     /locus_tag="OHN28_002666"
     CDS             complement(8534..9646)
                     /gene="ydiK"
                     /locus_tag="OHN28_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCQ2298044.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9860..12916
                     /locus_tag="OHN28_002667"
     CDS             9860..12916
                     /locus_tag="OHN28_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2298045.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12913..13323
                     /gene="menI"
                     /locus_tag="OHN28_002668"
     CDS             12913..13323
                     /gene="menI"
                     /locus_tag="OHN28_002668"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCQ2298046.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSVREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13423..13611
                     /locus_tag="OHN28_002669"
     CDS             13423..13611
                     /locus_tag="OHN28_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCQ2298047.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14160..14528
                     /gene="sufA"
                     /locus_tag="OHN28_002670"
     CDS             14160..14528
                     /gene="sufA"
                     /locus_tag="OHN28_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCQ2298048.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14537..16024
                     /gene="sufB"
                     /locus_tag="OHN28_002671"
     CDS             14537..16024
                     /gene="sufB"
                     /locus_tag="OHN28_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCQ2298049.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTVISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16034..16780
                     /gene="sufC"
                     /locus_tag="OHN28_002672"
     CDS             16034..16780
                     /gene="sufC"
                     /locus_tag="OHN28_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCQ2298050.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16755..18026
                     /gene="sufD"
                     /locus_tag="OHN28_002673"
     CDS             16755..18026
                     /gene="sufD"
                     /locus_tag="OHN28_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCQ2298051.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPTKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVMARIGQRLPGGAR"
     gene            18023..19243
                     /gene="sufS"
                     /locus_tag="OHN28_002674"
     CDS             18023..19243
                     /gene="sufS"
                     /locus_tag="OHN28_002674"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCQ2298052.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGVLYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALAQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19256..19672
                     /gene="sufE"
                     /locus_tag="OHN28_002675"
     CDS             19256..19672
                     /gene="sufE"
                     /locus_tag="OHN28_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCQ2298053.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19821..20825
                     /gene="ldtE"
                     /locus_tag="OHN28_002676"
     CDS             19821..20825
                     /gene="ldtE"
                     /locus_tag="OHN28_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCQ2298054.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20888..21124)
                     /gene="lpp"
                     /locus_tag="OHN28_002677"
     CDS             complement(20888..21124)
                     /gene="lpp"
                     /locus_tag="OHN28_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCQ2298055.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21435..22847)
                     /gene="pykF"
                     /locus_tag="OHN28_002678"
     CDS             complement(21435..22847)
                     /gene="pykF"
                     /locus_tag="OHN28_002678"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCQ2298056.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            23404..23613
                     /gene="fumD"
                     /locus_tag="OHN28_002679"
     CDS             23404..23613
                     /gene="fumD"
                     /locus_tag="OHN28_002679"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCQ2298057.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6165 a         5675 c         5981 g         5876 t
ORIGIN      
        1 aaatcacttt agagcagtta tcgaaaaaat ttcgtgccag cgttagttat atctgccatg
       61 aatttaccaa agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg
      121 aagcgaaatg gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg
      181 gttatgaaaa tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggc tgttcgccca
      241 gcgattaccg caggcaattt aaaaactgtt ttgcggaaca agaaatccta tctgaatttc
      301 ctcaaccagt aagtcttgcc ggataaatct ggcggcgcag tttgttaaga agctgcgccg
      361 cctgcagatt atttgttctg ataatctttc aggatctgcc gacctgctac gtaaatcata
      421 atttcgtctg taccgccgcc gatacgttca caacggacat cacgccagaa gcgggagacg
      481 cgcgcctcat cggtatagcc cagaccgccc atgatttgaa tcgcatcatc aatgacttcc
      541 attgcggtac gtgcgcagta cagttttgcc agcgccgcgc tggtgcgcag tgactgatgc
      601 tgatcggctt gccatgccac tttcaacacc atgttgcgca tgttgtcgat cttaatcgcc
      661 atcagcgcca gtttttcctg gatcatctgg ttatgaccaa tgggcttacc aaaagcgata
      721 cgctggttgg catagcgggc ggcatcttcg aatgcgcatt cggcaaagcc ggtgctgcgc
      781 gcggcgttga tcaggcgctc catctcaaag ttgtacatca cattgaggaa acccattcct
      841 tcttcgccca ccatgtcgct ctcttcaact tccacgttgt cgagatagac ttcgcaggtg
      901 ctgagcatat gccagccgat tttatgcagt ggattgattt taatgccggg cttactggag
      961 tcgacccacc acagagtaaa ggcctttttc ggatctttcg gttgcggatc gcgcgccaac
     1021 accagcatat atggatactc tttcgcaccg gtaataaagg ttttctgtcc gttgatgtaa
     1081 accttgccgt ttttacgcgt gtaagtggta gtggcactgt tgttgtctga gcctgcgcct
     1141 ggctccgtca atgccagggc ataggcggga tcacccgttt ccagggtgct ctctgccgtt
     1201 ttacgtagct gctctgcaga accgaaacgg cgcatactgt gaatacattg accgttggta
     1261 atcaaaaatg ccggagcacc gcattttgac acttccatca gcgccagcat ttgggtgacg
     1321 taatccgcag ggataccacc aaattcttcc ggcacgccaa gcatggaaat accgttatcc
     1381 gccagtgccc gcataaactc acgcgggtat gtcccgtttt gatcgcaggt gcggaaatac
     1441 tcttccggaa agttagtcgt aatcagttcg cgaatactgg ccagcagcag ttcttgttct
     1501 tcagttaaag agaaatccat cattatactc ctgttaatga gccgcttcag gcaggacaaa
     1561 acccatcgcc gcatcgataa ataatctttc gtccatcagt ttgagttctg gcgaaatcac
     1621 tggggtgaaa tccattttgt cgagaatatc tttttgtaaa tcgacgccag gagcgatttc
     1681 gattaaatgc aggccgtctt cttttagcgt gaatacggcg cgctcagtga tataacgaac
     1741 atccagccct cgctcgagag cgatttttcc gctgaaagtt atttccggta gttcccgaat
     1801 aaatttcttc acccgtcctt cctggacgat atttaatttg ccgtcggtaa tttctgtttt
     1861 taaactgccc gcagttaatg tgccgcagaa aacgattttc ttcgaagtgg cactgatatc
     1921 aataaatcca ccggtgccca tgattttacc attgaattta tgcacgccga cgttaccgtg
     1981 ctggtcgact tcagcaaaac tcaaataaca aacatccaga ccaccaccgt gataaaaatc
     2041 aaactgggac gtcatatcca gaatggcgcg ggtattcacg ttcgcgccaa aggcgatccc
     2101 ctgtgaagta ataccgccaa tcggacctgt ttctaccgtc agaataaagt catcagcaca
     2161 accttcttcc cgggcgacca ggccaatgcc gtcagcaata ccgacgccga cattccccac
     2221 tgcgcctttg cgcatttcga ataatgcacg ccgtgcaact aatttacgtt ggtttagggg
     2281 caggctaagt ttggtgctgt catcaagggt gaagtcacca gaaataaagc ggttaaccgg
     2341 cgcaccgcca tacagttggg tttgatccgg atcgaccacc acaatatcca ccagataacc
     2401 cggaatacgg acagatttag gatgcagcgt ggctttctta accattttct gcacctgcat
     2461 catcacaata ccgccgttat tgtgcaccgc ctgggcaata accaatgcgt cgagatacat
     2521 cacctcatct tcaaaagtgg cgtagccttc gctgtcgcag gtggtagcgc gaatgaaggc
     2581 gatatctggc gcaatcgctt tgtaatagag atattctttg ttatcaaact cgaccagttt
     2641 aatcaggtct tctttagtga cttcattcag tttgccgcct tgctggcgtg gatcgacaaa
     2701 tgttccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg cgcgtaaggt
     2761 ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt ctgcgagatc
     2821 agaaatacgc ggtgattgtc cccagtgacc gcataatgcc catttcacca ggccttcttg
     2881 cgccagcgga ctaataccac ggtcggcgcg atcgccaagc cctgttggac taataatcga
     2941 taaattacgt ggtgtttgag tctgtttata tttatcagca agagcagtaa ttaacgtggt
     3001 ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt cgggaatata
     3061 attcaccgct tcctgtgccg ataggaccgg cactcgtcca ttaatacgag gtggttttac
     3121 aggtttcatt gttaattcct gattcaggtg tgattttccc tacgccagat ggagaattag
     3181 cgcacagaga ctcacgaaat atttttatta aatacggtta aaaatacttt ttttagttcg
     3241 gcgggagagg gtattcccgc cgaaaatatt attgcttatg cctgatgtaa aatagttaat
     3301 accgtacgca aatcatttac cgagatttgc cctggcgcag aggctttttt taccgcacca
     3361 aaagttgccg ccgacccaaa tacttcacca gccagacgag aaattacgcc agtttttgcc
     3421 atcgacatcg tgatgattgg acgatcggca tactgctcct gcatctccag ggtcgcggca
     3481 agcaacgtca gcacatcgct ggtactttgc ggcatcagcg caatcttagg aatatcggcg
     3541 tcgaaggact gcattttgcg cagacgggca atgatttctt cggcttccgg cgttttatgg
     3601 aagtcatggt tggacatgac aactttcaca tcatgcgcgt gggcgtaggc gacggtttct
     3661 ttaacctgat catcgccggt aaataactcc agatcgatca tatcaaccag gccgctgtcg
     3721 atggctgcac gattgagtgc aatataagcc tcggtggaaa tcgcctgctc gccgccttct
     3781 ttggcactgc ggaaggtaaa cagcagcggt ttttctggca tggtttcacg gagaattttt
     3841 gctgccgcca tgacagactc cacattggag aggtcggcat agtggtccac acgccattcc
     3901 agaatatcaa agtccgcttc acgataggcg agagcttcgg atttcacgct ggcgatatct
     3961 ttcgccatca gcgagacgat gattttaggt gcgcccgtac caatgacgag atcttttaca
     4021 gttacggttt tcatttttta ccctttctgc acgcggtcag cctgtcaggc accgaacccc
     4081 atgacctgtt taacatattc cagagggaaa tctttgccag tccataacgt gaactgttca
     4141 gccccttgcc acaacaacat gccgtatcca tcaatcgttt tgcaacccgc ttgttgcgcc
     4201 tgctgcaata acttcgtcat atgagggtta tacacgcatt cagtgaccag aagtcccggg
     4261 tgtaacagac tgatatcatt aaccaatgat tcattctcaa ggggttccat acccactttg
     4321 gtgccattgg ttaaaatgtc ggcggaagcc agggcttctg caaaggcttg ctgatcggcg
     4381 agatcggtga ctgtcacgac gcaatcggtg ttttcgttaa cccgctgcgc gaaggcgaga
     4441 gctttatcga agaactcatc ccgacggtta aagagtttaa tttcttttaa accttcaatt
     4501 gccccctgcg cgccaattgc cgttgaggca ccaccggccc ctaatagcac catcgttttg
     4561 cctttgatat caaaaccgct ctctttaatg gcgcgaatat gacccgtgcc gtcggtgtta
     4621 tagccacgca gatagccatc atcatttacg atggtgttga tggcacccac cagtttggcc
     4681 gctggcgtta attcatcaac atattcacac gccagttgtt tgttcggcat tgatacacca
     4741 gttccgcgca ttttgagggc ttttaatcct tcaattgctc cgggaaaact atcgttatcc
     4801 acttcaaagg ccatataggt aaatggcaat cccgcttttt ctaaggcttt attctgcatt
     4861 tcgggcgata aactgtggcg gatgggatag gccatcaacc caatcaattc gtattttgcg
     4921 gtaacatcca taattaactc ctttaacctc tatgcttaat tgtgtttaac cgggtgctaa
     4981 atttatgctc gccaaaccgc acatcatttt caggaataat gaaaacgcgg taatagcgga
     5041 taaaaacaat aattgcggta atcagggcca gcagcgcgaa agtaaaatcg agaacaatga
     5101 tatattgcag gccgatgttc gacagataac cggtaatcag cggaataaca aagttagcca
     5161 gtccacccat catcatatat atactggtga ctttggcttt gctttttggg aaaaactctg
     5221 acatgaccga aacgccgagc tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg
     5281 ctccggcatt acacaccaat ggagatgggt acaggtagat aatggcagct gttattgttg
     5341 ccagtgcaga gttaaataca ttagcccaga tgggccggac catttttttc agcagtgcgg
     5401 caaaaataaa gacacagacc agcgaaccca tactgtaata agagatggtt tttaatgcct
     5461 cagcttctga cataccagca aaagccatcg catatttggg catccacacc acaatcacat
     5521 aaaaggtcga gaatgcggct acaccgaaca gtactgatga aacaccttcc agccagacta
     5581 acggtttgct gttcatttgc ggtaattcat tagctacgct ggcgtccacc aactggctgg
     5641 ggaatttgct tttcaacagc atcagcgtga tcagtacaaa cagaataccc ggaataatca
     5701 gcccatagcc gtaccagatg ttattgagca acatatagct caccagcatt ggatagaaca
     5761 tttgcccgaa tgacaccatc gctttaacca gtatgaccgc cgaaccagag gctttcggaa
     5821 agcattccat gagcgcgggg tagccacccg tatccagcgc tgagttagcg atacctacgc
     5881 acactgccag acagtaggcg agagttaaat tcgggcaagc aggaatacca aagaagaata
     5941 gcagatacat tattactgcc attaatatca ccgcccgacg accaaactta tcggagatca
     6001 ctccgaagaa taaaatactg atcaatcgcc ccaatccgat accggaaatt aaataggcaa
     6061 tgcccgcgtt gtcagtggaa aacttttctg ccaaagatgt catgttttgg gcaagcgtaa
     6121 taacactaat gccgtgcagg aaatagctga agtaaataca aagaacagcc aggataaatg
     6181 gcgtgctgaa agccttattt tgagacatgt tcacagctcc ttgctgaaag ataatttcgt
     6241 taccgtacgt attgttctcc gacggtttac ataattacta tactgaagat gattttccct
     6301 gggtacatgg cataaagttt atgtttccag gaatattgca tgtcatagtt tgtttttgat
     6361 cgctattttc cttagttagc gcagaaagag ttatttatcc gtaagatctt gcataacctg
     6421 aaaagaatat tgcattaccc acccggagcg atattttcct ttagcgtgtg atgccgcgtt
     6481 tctttgcgac tgcgtaagcc aataaacagt gccagtataa agccgatagc agcgatggcg
     6541 gtatcgaacc acataatatc ggcaatactt ctttgcgaaa gatgagccgt aatcaacgga
     6601 atggtaaatg ttgcaatgct gcccgcactg taatagatcc ccgtagcttt acctttagcg
     6661 tagggaaaac gttccgccat taacgtcagg ccaatttgta caacaccgcc tgcggaggta
     6721 aaaccgatga cgaaagcaaa tattatcacc acataaaatg tgggatgcag gcagacggtg
     6781 agcagggcga taaatgagat aaaggtgtac agcatcagta atgttgttgg gcgaacggta
     6841 ttacgaatga gtggagcagt aataaataca cacagcagcg aacccacggt gtagatactg
     6901 agtaatttga tcgacatggt gtatgacatg ccagcgacaa attgtccgta ttgtgccagc
     6961 cactggctaa ccagataaaa cgttgccatt gagatatagc catataggga ataactggct
     7021 aaatcgataa ttgaacagcg atgttccgta gagctggtgg ttttctttat gacaggtaag
     7081 tgacgacccg gatggggtgg gaacgtacaa cggtataaaa acagagcgtt aataaacata
     7141 atgcctgcgg caatcataaa ggaccaaccg aaccacagtt cagcccacac taacaggctg
     7201 ataattagcg gtaataaaaa ttgtccgctg gaaacaaatg ctttaattaa aatattggct
     7261 gtacccggtg agcgtggaaa agcttccatc aaactgggat aggtgcctgc atcgagaaaa
     7321 ctgtttgcca ttcccgccag aaagccaaaa acataagcga taatgatgtt attggtgtgc
     7381 aggatgccaa aaaagaaggc catatagcag cacatcccga gcatgataaa agggcggcga
     7441 ccaaagcgat cggataataa tcctgcaaaa agcaagacac ttaatcgacc aatgcccagt
     7501 gatgagataa ctatcgagac gcccgcggca ttagtctgcc aaagcgtctc cagcgaggcc
     7561 atattcaggc tcatcaaaat gacgcccata ccatgcacca ggtaattaaa atacaaccca
     7621 agtgcggtag ggtaataggg atttttcata ggttctactt agcaaaggtg ctttaaaaag
     7681 tgtagcgatg ttgtgtcagt atccgttcat tattgttgta acaacgacaa ttcctttctg
     7741 tggagaaatt aacaaagccg ctccagatcg tgtgcatata attcagcggc taccgattga
     7801 tagatcttcc agtcaataaa attgccatca gggtagactt gttgaaacgc ggttaagtcg
     7861 gggaaaaaac gcatggtgtt gttgccgatg cggttattta ttttctctat gattgcatga
     7921 agatgttttt ttcgttgctg acgcatagcc aacagttgct cgactacata atcaggaatg
     7981 gcgcatttgc cattttccca ctgacgccag ctttcactgt taccggtctg ggcaatccag
     8041 gtcgcgcatt catcaatagt catggcaaaa atatggcgaa gtgcctggag ttcataagcg
     8101 ttcatcgtgt aatggctgca tgaaataaac aataaaatat aaataacatt atcagcctgc
     8161 tgacggcttg aagagagtca cagtatcttg tgcaacatta ttggaaagag tgaggggcga
     8221 ggtagcgaag cagaaaaatg ttaaaaaaaa gcccatcgtg ggagatgggc aaagactaca
     8281 cacagcaatt cgttgtttca ctcaggggat ttccatgctt ataaatcaat atgttgattt
     8341 ataaccgtga gctaatagta ggcatacgga ctttttgcgt cgatcagatt cgtctcatta
     8401 gtttaaaaac tgtaaagaat ttgcgacagg tactaccagc tgaatagcgg taagcaggaa
     8461 aatatgggga agagcaagca aatatcaatt agcaggggtt aacaatcagg cggcagagcc
     8521 gcctgatgag aaattactta ttcgatttct cgatttcgcc aagttcttcg agaatttctt
     8581 ccggttggtc cgtcggcggt gggacttctt ccacccacgc ggcaaacaga cgccaggaaa
     8641 cggctaacag aaccggacca ataaacagac cgatcatccc gaaagcaatc aaaccaccaa
     8701 taacgccaga gaggatcagg atcagcggta aatcggcacc catgcgaatt aacattgggc
     8761 ggatgacgtt atcgagcgtg ccaaccacac cgctccaaac taacaatacc gttccccagg
     8821 tggtatcgcc agtccagtag agccagataa tcgccggaat cagtaccggc aacggtccaa
     8881 gctggacaag gcaggagagg atcattaaca ccgttagcaa agttgcataa ggtacgccgg
     8941 atacggcgag gccgataccg ccaagcactg cctgtactaa cgccgtcacc accacaccca
     9001 gcgccaccgc gcggatagcc tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg
     9061 ccagacgggt tgcaaaatgg cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca
     9121 gggcgctgaa gagaagcatc agcgcacaat gcaccataaa gcgcccgata tgcgccgcct
     9181 gcccaacgaa ccaggtggtg gtggtgccaa tataaggacg gactttcgcc atgatcgccg
     9241 tcccccccat atccagcaaa ttgtgccagc ctgcatatag cttcgcgcca atcaccggaa
     9301 tggtattaag ccacgccaga tcgggtaacg tcatgtcacc actggaaatg gctttaatta
     9361 gcgggccgct gccgtcgacg atactgttaa ccagcaaagc aatagggatg ataaacacca
     9421 tcactaataa cagcgtcatc accagaacgg cgagagagcg gcgaccaaac atgatctttt
     9481 gcaaacgtaa caataccggc caggtggcga taaccactgt accggcccat gcaaagccga
     9541 gaataaaggg ttgtacaatc cacagacatg ccacaatcat gatggctaaa aacagcaccg
     9601 aaagcagaat ttgtgcgaca tccctgggct gacgaacatt taccataact acttttcacc
     9661 ttttttgtgc gtcaaatcgt cgacgcgacg tgaacacgcg aaactcacct gcataatgat
     9721 gagcaatttc agcggttttt aacaggtcga ttctgccctt aattctgatg ggcgtgttag
     9781 aaaaaaatgt ggtacaacaa ctcaggcaac acgcaaacga ttaatctctc atcacagacc
     9841 gcaggaaagg gtcaatataa tgattccaca gatttcccag gcacccggcg tcgttcaact
     9901 ggtgcttaat tttttgcaag agctggagca acagggtttt accggcgata cggcgacaag
     9961 ttatgccgat cgtctgacaa tgtcgactga caacagtatt taccaacttc tccccgatgc
    10021 ggtggtattt ccgcgttcaa ccgcagatgt agcgctgatc gcccgtcttg ccgcgcagga
    10081 acgctattca tcgttgatct ttaccccccg cggcggcggc accggcacta acggtcaggc
    10141 gctcaaccag gggattattg ttgatatgtc ccgccatatg aaccgcatca tcgaaattaa
    10201 ccctgaagag ggctgggtgc gcgttgaggc cggggtgatt aaagaccaac tcaatcagta
    10261 cctgaaaccc tttggctact tttttgcgcc agaactttcg accagcaacc gggcaacgct
    10321 cggcgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg gcaaaacgtc
    10381 agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg atacgcaacc
    10441 tttacccgtc gaactggcgg aaacgctggg taaatccaat accacaatcg ggcgaattta
    10501 taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca acttccccaa
    10561 gcttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg agatgaccga
    10621 gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta ttaccgaagc
    10681 gcgactggat attacgcgct tgcctaaagt acgccgtctg gtgaacgtca aatatgactc
    10741 ttttgactcc gcgctgcgta acgcgccgtt tatggttgag gcgcgcgcgc tttcggtaga
    10801 gacggtggac tcaaaagtgc tcaaccttgc acgggaagat attgtctggc attccgtcag
    10861 cgagttgatt accgatgtgc ctgataaaga gatgcttggg ctgaacattg tggaatttgc
    10921 cggtgatgat gaggcactga ttgacgagcg agttaatgca ctctgtgtgc ggcttgatga
    10981 gctgattgcc agccaacagg caggagtgat cggctggcag gtgtgccgcg agctggcggg
    11041 tgttgaacgt atctatgcga tgcgcaaaaa agccgttggt ctgcttggca atgccaaagg
    11101 tgctgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac acctggcgga
    11161 ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg gtatgttcgg
    11221 tcacgtcgac gcaggtgtct tgcacgtccg tccggcgctg gatatgtgcg atccgcaaca
    11281 agagattttg atgaagcaaa tctctgatga tgtagtggcg cttaccgcga aatacggtgg
    11341 tttgttgtgg ggcgaacacg gcaaaggttt tcgcgccgaa tacagcccgg cgtttttcgg
    11401 tgaggaactt tttgcagaac tgcgtaaagt gaaagcggca tttgacccgc ataaccgact
    11461 caaccccggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga aagtggacgc
    11521 ggtgaagcgc ggtacattcg atcgacagat ccccattgcg gtacgccagc agtggcgtgg
    11581 tgcaatggag tgtaacggca acggtttatg cttcaacttt gatgctcgta gtccgatgtg
    11641 tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg ctacgctggt
    11701 acgtgaatgg ctgcgtttgt tggcggatcg cggcgttgac ccactcaaac tggaacaaga
    11761 acttcctgaa tccggagtca gtttgcggac gttaattgcc cgcacgcgca atagttggca
    11821 tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt cgggctgtct
    11881 ggcctgtaaa gcgtgttcga ctcagtgccc catcaaaatt gatgtgccgg agtttcgctc
    11941 tcgtttcctg caactctatc acacccgtta tttacgcccg ctgcgcgacc acctcgtcgc
    12001 tacggttgag agttacgcac cgctgatggc acgcgcgccg aaaaccttta atttcttcat
    12061 taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg atttgccgct
    12121 gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa acatgacgct
    12181 ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgcttg tggtgcagga
    12241 cccctttacc agctattacg atgctcaagt ggtggcggat tttgtccgtc tggtcgaaaa
    12301 attaggtttc cagcccgtgt tactgccatt ttcgccaaat ggcaaagccc agcatatcaa
    12361 aggttttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc tcaaccgtat
    12421 ggcgaagctg ggtatgccaa tggtaggcgt cgacccggcg ctggtacttt gttatcgcga
    12481 tgaatataaa ctggctctgg gcgaggagcg tggcgagttt aacgtcttac tggcgaatga
    12541 atggctggca agcgcacttg agtcacagcc agtggctaca gtcagcggtg aatcatggta
    12601 tttctttggt cactgtaccg aagttaccgc cttgccgggc gcgccagcac aatgggcggc
    12661 aatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtggggtgct gcggcatggc
    12721 agggacttac ggacatgaag cgaaaaatca taaaaattcg ctcgggatct atgagttatc
    12781 ctggcatcag gcgatgcagc gactgccgcg taatcgctgt ctggcgaccg gatattcctg
    12841 ccgtagtcag gtaaaacggg ttgaaggcac gggggtacgc catcctgtgc aggctttact
    12901 ggagattatt aaatgatatg gaaacgaaaa atcaccctgg aagcactgaa tgctatgggg
    12961 gaaggaaaca tggtggggtt gctggatatt cgctttgaac atattggtga tgacaccctt
    13021 gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct gcatggaggt
    13081 gcatccgtgg tactggccga aagtatcggt tccgttgccg gttatttatg taccgagggt
    13141 gagcaaaaag tggttggtct ggaaatcaat gctaaccacg tccgctcagt acgagaaggg
    13201 cgggtgcgcg gcgtatgtaa accgttgcat ctcggttcgc gtcaccaggt ctggcagatt
    13261 gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac cgctattttg
    13321 tgacgttgtc accagaaaag tgtgacgcat attgcttttg gttattatct gcacagtaaa
    13381 cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac tcaacttgat
    13441 cccacccaac tggccattga atttttacgc cgtgaccaaa gcaatctttc acctgcccaa
    13501 tatctcaagc gtctgaaaca attagaactt gaatttgctg acctgcttac tctctcttct
    13561 gctgaattaa aagaagaaat ctatttcgcc tggcgtctgg gcgtgcattg atcctgattt
    13621 aatgtcgcga gttcgccaaa tcggcctgga ttaatcttcg tccaggttcg agggccttct
    13681 taatactgcc tatctacccg cctgataacg ttcaaaaata atacaaatcg cagtttgtgt
    13741 taaaacagtg ggttagcttt atgagattat ccttatcgcc ccttcaagag ctaagccact
    13801 gtgagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg tcgcacagac
    13861 ttaagggttt tcttatttct gaacgctgca cttttttacc tatcccttaa atagtcattt
    13921 tcaatgcaac tttttctttt tcatcacagc cttaatgctc cttagataat aatcattatc
    13981 taacaatgag atacctaatt cttagcgtgc ctgttaaccc acaaatcagg gtctatgctt
    14041 attaaaagca ggtaaaaagc atgttttacg ctaaagcccc tgcgtttgct gggttgaact
    14101 gataatcatt atcactaaca tgctgttata cgctgaaagc gatgaagtga ggtaaatcga
    14161 tggacatgca ttcaggaacc tttaatccac aggatttcgc ctggcaaggc ttaacgctga
    14221 cacccgcagc ggcgatacac atccgcgaac tggtggcaaa gcagccgggt atgatcggcg
    14281 tgcgcttagg cgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc gacagtgtta
    14341 gcgagccgga caaagacgat ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc
    14401 tgcaagcgat gccttttatt gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc
    14461 agatattcaa atttcacaac cctaaagccc agaatgaatg tggctgtggc gaaagctttg
    14521 gggtataggc ggtactatgt ctcgtaatac tgaagcaact gacgatgtca aaacctggac
    14581 cggcggcccg ctgaattata aagaaggatt cttcacccag ttagccaccg atgagctggc
    14641 aaaggggata aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg
    14701 gatgctggag tttcgtctca acgcctatca cgcatggctg gagatggaag aaccgcactg
    14761 gttgaaagcg cactacgaca agctgaatta tcaggattac agctactact cggcaccatc
    14821 gtgcggtaat tgtgacgaca actgcgcgtc tgaacccggc gcggtgcagc aaactggcgc
    14881 gaacgccttt ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg
    14941 ggaaggcaaa gaggtggcgg tggatgccat tttcgactct gtttcggttg ccactactta
    15001 tcgcgaaaaa ctggcggagc agggaattat tttttgttcc tttggcgagg cgatccacga
    15061 tcacccggaa ctggtgcgta aatatctcgg caccgtggtg ccggggaatg acaacttctt
    15121 tgccgcactt aatgcggcgg tagcctctga cggtacgttt atttatgtgc ctaaaggcgt
    15181 gcgttgcccg atggaacttt ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt
    15241 tgagcgcacc attctggtgg ccgacgaaga cagctacgtt agctacattg aaggctgttc
    15301 cgctccggtg cgtgacagct atcagttaca cgcggcggtg gtggaagtca tcatccataa
    15361 aaacgccgag gtgaaatatt ctacggtaca aaactggttc cctggcgata acaacaccgg
    15421 cggtattctc aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc
    15481 atggacgcaa tcagaaaccg ggtcagcgat tacgtggaaa tatcccagct gcattttgcg
    15541 cggcgataac tccattggtg agttttactc agtggcgctg accagcggtc atcaacaagc
    15601 ggataccggc accaagatga tccacatcgg taaaaacacc aaatcgaccg ttatctcgaa
    15661 agggatctct gccggacata gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac
    15721 ggcaaccaat gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg ctaattgtgg
    15781 ggcgcatacc ttcccgtatg tcgaatgtcg caataacagc gctcagctgg agcacgaggc
    15841 aacgacatca cgtattggtg aagatcaact gttttactgc ctgcaacgcg ggatcagcga
    15901 agaagatgcc atctcgatga ttgttaacgg tttttgcaaa gacgtgttct cggaactgcc
    15961 gctggaattt gccgttgaag cacaaaaact cctcgccatc agccttgagc atagcgtcgg
    16021 ataaggaata aacatgttaa gtattaaaga tttacacgtc agcgtggaag ataaagctat
    16081 cctgcgtgga ttaagcctcg atgttcgtcc cggcgaagtt cacgccatta tggggccaaa
    16141 cggttcgggc aaaagtacct tatcggcaac gcttgccggg cgagaagatt atgaagtgac
    16201 gagcggcacg gttgagttta aaggcaaaga tttgcttgcg ctgtcgccgg aagatcgtgc
    16261 aggtgaaggc atctttatgg ccttccagta tccggtggaa attcccggcg tcagtaacca
    16321 gtttttcctg caaacggcac ttaatgcggt gcgcagctat cgcggccagg aaacgctcga
    16381 ccgctttgat tttcaggatt tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga
    16441 tttattaacc cgttcggtaa acgttggttt ttccggcggc gagaaaaagc gcaacgatat
    16501 tttgcaaatg gcggtgctgg aaccggagtt atgcattctt gatgagtcag actccgggct
    16561 ggatattgac gcattaaaag tggttgccga tggcgtgaac tcgctgcgtg acggcaagcg
    16621 ttcattcatc attgttacgc actatcaacg cattctcgac tacatcaagc ctgattacgt
    16681 ccatgtgctg tatcagggac gaattgtgaa atccggcgat ttcacgttgg tcaaacaact
    16741 ggaggagcag ggttatggct ggcttaccga acagcagtaa cgcactgcaa cagtggcatc
    16801 atttgtttga agctgaaggg gcgaaacgct ccccgcaagc acagcagcat ttacaacaat
    16861 tgctgcgtac cggactgccg acacgtaaac atgaaaactg gaaatatacg ccgctggacg
    16921 ggctgaccaa tagccagttt gtctgcattg cgggggagat atccccacag cagcgtgatg
    16981 ccttagcgtt aacgttagac gccgtgcggc tggtgtttgt cgatggacgt tacgtgcccg
    17041 cactgagcga tgcaactgaa ggcagcggat atgaagtgag cattaacgac gaccgtcagg
    17101 ggttacccga cgctattcag gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa
    17161 gcgtgacgca tatcgccgtg aagcgcggtc aacggccgac aaagccattg ctgttaatgc
    17221 atatcaccca gggcgtggca ggtgaagagg tgaatactgc ccattaccgg catcatctgg
    17281 atctggcgga aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt aatgatgctc
    17341 gtcacttcac cggcgcacgg ttcactatca acgtcgcagc gaacgcccat ttgcagcata
    17401 tcaagctggc gtttgaaaac ccgctcagtc accactttgc tcataacgat ttgttgctgg
    17461 ctgatgatgc caccgcattt agccacagtt tcctgctggg tggcgcagtg ttacgacaca
    17521 acaccagtac gcaactcaat ggcgaaaaca gcacggtgcg gatcaatagc ctggcgatgc
    17581 cggtgaaaaa cgaggtgtgt gatactcgta cctggctgga acacaataaa ggtttttgta
    17641 acagccgaca gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta tttaacggtt
    17701 tgatcaacgt cgcgcagcac gccatcaaaa cggatggtca gatgaccaac aataatctgc
    17761 tgatgggcaa actggcggaa gtggatacta aaccgcagct ggaaatctat gcagatgatg
    17821 tgaaatgcag ccacggcgcg acggtggggc gtattgatga cgaacagatg ttttatctgc
    17881 gctcgcgcgg gatcaatcag caagatgccc agcagatgat catctacgcc tttgctgccg
    17941 aactgacgga agcactgtgt gatgaggggc ttaaacagca ggtgatggcc cgaatcggtc
    18001 aacgactgcc aggaggtgca agatgacttt ttccgtcgac aaagtgcggg ccgactttcc
    18061 ggtgctttct cgtgaggtaa acggtttgcc gctggcttat ctcgacagtg ccgccagtgc
    18121 gcagaaacca agccaggtga ttgacgccga ggctgagttt tatcgtcacg gctacgcggc
    18181 ggtgcatcgc ggtattcata ccttaagcgc ccaggcgacc gagaaaatgg agaacgtgcg
    18241 caagcgggca tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt tcgtccgcgg
    18301 cacgacggaa gggatcaatc tggtcgccaa tagctggggc aacagcaatg tgcgagcggg
    18361 cgataacatc atcatcagtc agatggagca ccacgctaac attgtcccct ggcagatgct
    18421 ttgcgcacgc gttggcgcag agctgcgtgt gatcccgctc aaccccgacg gtactctgca
    18481 actggagacg ctgcctacgc tgtttgatga gaaaactcgc ctgctggcaa ttactcatgt
    18541 ctccaacgtg cttgggacag aaaatccgct ggcggagatg atcacacttg cgcatcaaca
    18601 tggcgcaaaa gtgctggtgg atggcgctca ggcggtgatg catcatccgg tggatgttca
    18661 ggcgctggat tgcgatttct acgtgttttc cgggcataaa ctgtatggtc ccaccggcat
    18721 tggcgtgctg tatgtcaaag aagccttgtt gcaggagatg ccgccgtggg aagggggcgg
    18781 ttctatgatt gccaccgtta gcctgagtga aggcactacc tggaccaaag cgccgtggcg
    18841 gtttgaagcc ggtacgccca ataccggagg tattattggc cttggcgcgg cgctggagta
    18901 tgtttcggcg ctggggctta ataacatagc cgagtatgaa cagaatctga tgcactacgc
    18961 gctagcacag ctggaatctg taccggatct cactctctat ggcccacaga acaggcttgg
    19021 cgttattgct tttaatctcg gtaaacacca cgcctatgat gttggcagtt ttctcgataa
    19081 ttacggcatt gctgtgcgta ccggacatca ctgcgctatg ccattaatgg cctattacaa
    19141 cgtccccgcg atgtgtcggg cgtcgctggc aatgtacaac acccatgaag aagtggatcg
    19201 tctggtaact ggattgcaac gtattcatcg tctactgggg taaaagggag gctctatggc
    19261 tgtattgccg gataaagaaa aattgctgcg taacttttta cgctgcgcca actgggaaga
    19321 gaaatatctc tacattattg aactgggaca gcgtctgcca gaattacgtg acgaagacaa
    19381 aagcccgcaa aatagcatcc agggctgcca gagtcaggtg tggattgtaa tgcgccagaa
    19441 tgcgcaggga attattgaat tgcagggcga cagcgatgcg gccattgtaa aagggcttat
    19501 tgcggtcgtc tttattcttt acgatcaaat gacgccgcag gatattgtca atttcgatgt
    19561 gcgcccgtgg tttgaaaaaa tggcgctcac ccaacatctc accccatcac gctcacaagg
    19621 tctggaagcg atgattcgcg caattcgcgc caaagccgct gcacttagct aaactaaagg
    19681 gacagctttc atccggttgg ctcacattgt cgggtggtca atgtttacac tggcctgcac
    19741 aggagtgttt tggtttcagg tgaacataag cgccatccga cgtttcagcg tgagtctccg
    19801 gcaaatacag gaggtttaca atgaaacgcg cgtctttgct tacactcacg cttatcggcg
    19861 cttttagcgc catccaggct gcctgggcgg ttgattatcc gctaccacca accggcagcc
    19921 gactggttgg gcaaaatcaa acgtatacgg tgcaagaagg ggataaaaac cttcaggcca
    19981 tcgcccgacg ttttgatact gcggcaatgt tgatccttga agccaataac actatcgccc
    20041 cggtgccaaa acccggtacg acgataacta ttccttcaca actgttatta cctgatgcac
    20101 cgcgccaggg gataattgtg aaccttgcag aactgcgcct ttattattat ccgccgggag
    20161 aaaatatcgt gcaggtttat ccgataggta ttggtttgca ggggctggaa acgccggtga
    20221 tggaaacgcg tgtggggcag aaaatcccta acccaacctg gacgcctacg gcgggcattc
    20281 gtcaacgttc gctggagcgt ggcattaaat taccgccggt cgttcctgcc gggccaaata
    20341 acccgctagg acgttacgca ctgcgcctcg cgcatggtaa tggcgaatac ctcattcatg
    20401 gcaccagtgc gccggacagc gtcggtttgc gcgtcagttc gggctgtatt cgcatgaatg
    20461 caccggatat taaagcttta ttctccagcg tgcggacggg aacgccggtg aaagtgatca
    20521 acgaaccggt gaaatattcc gtggaaccta acgggatgcg ttatgttgaa gtacatcgac
    20581 cactatcggc agaagaacag cagaacgttc agacaatgcc gtacacgctg ccagcaggct
    20641 ttacgcaatt taaagacaat aaggctgtag atcagaagtt agtcgataaa gcgttgtatc
    20701 gtcgggcagg gtatccggtt gcggtgagca gtggagcaac tcccgcagcc agcaatgcgc
    20761 cttcagtaga gtcagcgcag aatggggaac cagagcaagg gaatatgtta cgcgcgacgc
    20821 agtagcggta aatagcaggc aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca
    20881 ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg
    20941 cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt
    21001 caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg
    21061 agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt
    21121 tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa
    21181 agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc
    21241 aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaacag gatgcttcca
    21301 tcggattcat cttagataaa gtgagattat atagtgaaat ccgatttgat tttttattgc
    21361 ttctggttat cgattaaata aaaaaagcgc ccatcaggac gcttcgatat acaaattaat
    21421 tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc
    21481 agtgcaccag aaaccataac tacgacatca cctttgtgtg ccagaccgct ctgcagagcc
    21541 agttctttac ccagacggta gaagtcatca gtagaagtga tctctttaac cagctgcggc
    21601 acaacgcctt tgctcagtac taactggtga gccgtttttt cgttggtggt cagtgccagg
    21661 atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgagtagca
    21721 accacgatca gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct
    21781 tcggttatac gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg
    21841 tcggtacgtt cgcagatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt
    21901 gcggattcac cagacagcat cactgcgtca gtaccgtcga ggatggcgtt tgcaacgtca
    21961 ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca
    22021 gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata
    22081 acttcttcta ccgggatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag
    22141 gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg
    22201 tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc
    22261 ttacgaataa aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc
    22321 tgtttgtctt tttcagccag tgctggcagg gcaatggaaa cgccaggcag gttcacacct
    22381 ttgttttcgc ccaggtcgcc gttgttcagc actttacaga taactttgtt accttcaatg
    22441 gcggtaactt ccataccgat cagaccgtcg tcaaccagta cggtgttgcc aacagacagg
    22501 tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta
    22561 tcagtagtga aagtaaaggt ctgacccgct ttcagagaaa cgtcgttacc gccttccagt
    22621 ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt
    22681 ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga
    22741 gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca
    22801 gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc
    22861 tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga aagcgaatcc
    22921 ggtattacga aagtggttgt gcctcgctct aaggataggt gacatcgaaa aagcgtgcag
    22981 aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc gttgagttct
    23041 acagtccaaa gtgctgaaac cattcaagag tcaattggcg cgcattatac gctgaaatta
    23101 atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac aacttgttag acaataaatt
    23161 gttatcaaag attcagcgtt ccagggaaaa ggttacgcaa aaacttcgtt gtttgcgcaa
    23221 cgtctggtgc cggggttgtc agtgtgagga taagtgaaag ttcatccggt gtataaatac
    23281 atcatcgctc aattgctaca gttaaaataa tttatcacgt tatttgtctg attttgtttg
    23341 atttttgttt aattggttag attgatatat gctgatagtt ggcatgataa ataggtataa
    23401 agaatgggaa accggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt
    23461 aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc
    23521 gtattacgta cagcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg
    23581 atggaaacag tcattaacgc actagtaaaa tgaccattca ggatgaggcg gtaagcttca
    23641 tcctgatatt gttagttcag ttgcggtcca gttaatcatt ctaaataaca actgctg
//
LOCUS       DAJOTB010000071        23343 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJOTB010000071 DAJOTB010000000
VERSION     DAJOTB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.071"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            215..1066
                     /locus_tag="OHN28_002680"
     CDS             215..1066
                     /locus_tag="OHN28_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2298058.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGFPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            1059..1430
                     /locus_tag="OHN28_002681"
     CDS             1059..1430
                     /locus_tag="OHN28_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2298059.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1427..2179
                     /gene="fliP"
                     /locus_tag="OHN28_002682"
     CDS             1427..2179
                     /gene="fliP"
                     /locus_tag="OHN28_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCQ2298060.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2182..2454
                     /gene="fliQ"
                     /locus_tag="OHN28_002683"
     CDS             2182..2454
                     /gene="fliQ"
                     /locus_tag="OHN28_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HCQ2298061.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2456..3238
                     /gene="fliR"
                     /locus_tag="OHN28_002684"
     CDS             2456..3238
                     /gene="fliR"
                     /locus_tag="OHN28_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCQ2298062.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHNLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3228..4367
                     /gene="flhB"
                     /locus_tag="OHN28_002685"
     CDS             3228..4367
                     /gene="flhB"
                     /locus_tag="OHN28_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCQ2298063.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLSLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4351..6444
                     /gene="flhA"
                     /locus_tag="OHN28_002686"
     CDS             4351..6444
                     /gene="flhA"
                     /locus_tag="OHN28_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCQ2298064.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(6664..7162)
                     /gene="rayT"
                     /locus_tag="OHN28_002687"
                     /pseudo
     CDS             complement(6664..7162)
                     /gene="rayT"
                     /locus_tag="OHN28_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(7338..8087)
                     /locus_tag="OHN28_002688"
     CDS             complement(7338..8087)
                     /locus_tag="OHN28_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCQ2298065.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            8399..9139
                     /locus_tag="OHN28_002689"
     CDS             8399..9139
                     /locus_tag="OHN28_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCQ2298066.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9110..9877)
                     /locus_tag="OHN28_002690"
     CDS             complement(9110..9877)
                     /locus_tag="OHN28_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCQ2298067.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10082..10660)
                     /gene="lpcA"
                     /locus_tag="OHN28_002691"
     CDS             complement(10082..10660)
                     /gene="lpcA"
                     /locus_tag="OHN28_002691"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCQ2298068.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            10900..13344
                     /gene="fadE"
                     /locus_tag="OHN28_002692"
     CDS             10900..13344
                     /gene="fadE"
                     /locus_tag="OHN28_002692"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCQ2298069.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(13387..13833)
                     /gene="ivy"
                     /locus_tag="OHN28_002693"
     CDS             complement(13387..13833)
                     /gene="ivy"
                     /locus_tag="OHN28_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCQ2298070.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            14014..14784
                     /gene="yafV"
                     /locus_tag="OHN28_002694"
     CDS             14014..14784
                     /gene="yafV"
                     /locus_tag="OHN28_002694"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCQ2298071.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            complement(14883..14959)
                     /locus_tag="OHN28_002695"
     tRNA            complement(14883..14959)
                     /locus_tag="OHN28_002695"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14923..14925),aa:Asp,seq:gtc)
     gene            complement(15092..15823)
                     /gene="dnaQ"
                     /locus_tag="OHN28_002696"
     CDS             complement(15092..15823)
                     /gene="dnaQ"
                     /locus_tag="OHN28_002696"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCQ2298072.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            15888..16355
                     /gene="rnhA"
                     /locus_tag="OHN28_002697"
     CDS             15888..16355
                     /gene="rnhA"
                     /locus_tag="OHN28_002697"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCQ2298073.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(16352..17074)
                     /locus_tag="OHN28_002698"
     CDS             complement(16352..17074)
                     /locus_tag="OHN28_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2298074.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            17108..17863
                     /gene="gloB"
                     /locus_tag="OHN28_002699"
     CDS             17108..17863
                     /gene="gloB"
                     /locus_tag="OHN28_002699"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCQ2298075.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            17935..19293
                     /gene="mltD"
                     /locus_tag="OHN28_002700"
     CDS             17935..19293
                     /gene="mltD"
                     /locus_tag="OHN28_002700"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCQ2298076.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(19342..20112)
                     /locus_tag="OHN28_002701"
     CDS             complement(19342..20112)
                     /locus_tag="OHN28_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2298077.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(20190..20990)
                     /locus_tag="OHN28_002702"
     CDS             complement(20190..20990)
                     /locus_tag="OHN28_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2298078.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            21231..22145
                     /locus_tag="OHN28_002703"
     CDS             21231..22145
                     /locus_tag="OHN28_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2298079.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(22142..22945)
                     /gene="dkgB"
                     /locus_tag="OHN28_002704"
     CDS             complement(22142..22945)
                     /gene="dkgB"
                     /locus_tag="OHN28_002704"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCQ2298080.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(23108..23184)
                     /locus_tag="OHN28_002705"
     tRNA            complement(23108..23184)
                     /locus_tag="OHN28_002705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23148..23150),aa:Asp,seq:gtc)
     gene            complement(23239..>23343)
                     /gene="rrf"
                     /locus_tag="OHN28_002706"
     rRNA            complement(23239..>23343)
                     /gene="rrf"
                     /locus_tag="OHN28_002706"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5644 a         5912 c         6112 g         5675 t
ORIGIN      
        1 gcctgatgcg acgctgcgcg tcttatcagg cctacagttt gtgcaatgtt ttatgacgat
       61 gcccgatatc tcgcccgtta ataatttaat tattttttca ccctattaat aatttgcccc
      121 tttcactccc cctctattta atacctcacc ttatgtcacc gccggatttg cgtgcacaga
      181 aacgcccata ccattacagc agagaaagta agccatgctg aagtacagta aaacgccagg
      241 catctttaaa ttagaaggta atcggcttgg acgcccctac catcgcctgc cgaccctgtt
      301 tacggggaat tttgatgtaa ttgattcgca tcttggcagc tactttctca aaaagcatcg
      361 cagcaatatc accctaaaaa agattggctg cgaaatggat attattaata aaaatgccga
      421 gctgatggtt tcccaggtcg ggcatctggc gtttgatatt gatcgctcct tgctgttaat
      481 gctgctgggc aatttttatg gtctggagtc gtctcttgaa gaagcgaaat cacacaatgg
      541 attcccgacc aaaacggaaa cacgtttaaa aaaccgcctc gcgctggata tctgcacgct
      601 gatttttaat ctgcaaacct ccggtattgc gctgaaatta aaactggaca gcagcacggt
      661 aattacccac tgggcgtatc aattaacctt tacccttgcg ggcgatgaag agagcagttt
      721 tcgtattttg ctcgacgacg cccacaccga ttttattttg aatctgatcc gccacagcga
      781 gcacagtaaa ccgcagcagg tggcgaaatc cgttaataaa ccggccctga ttaaagaaat
      841 catccgctcc ctgccgctga ccctgaacgt caaaatcgcc gagctatcga tgaatgtcgc
      901 ggatctggca cagattaaag ccggggatat tttacccata tcactgggag aaacgttccc
      961 ggtggcgatt gggcaatctg aattatttag cgccctgatt gtggaagaca aagacaaact
     1021 gtttttgtcg gagctggcgg gaaaaaatga gaacaaccat gagtaagcaa gaagatattt
     1081 tagccgggga ttttggcctc accgatgaag ttgcgcctgt cgccaaatcc gccgacgccg
     1141 aaacgctggt cacccgcctg gaagaccgat tttccgactc gatgacgcta ctgaaacgca
     1201 ttccggtgac cttaacgctg gaagtatcgt cggtggaaat catgttagcc gacctgctta
     1261 acatcgacga cgacacggtg attgaactgg ataaactcgc cggggagccg ctggatatta
     1321 aagtgaacaa tatcctgctg ggcaaagcgg aagtggtggt ggtcaacgag aagtacggcc
     1381 tgcgcgtgct ggaatttaac acccgcgata tcaacgacct ggcgccatga aacgccgcac
     1441 tcaactgacg ctgggcctgg cgctgctggc gctcgccccg ctggcgctgg cccagggcgg
     1501 cgatatcgca ctgcttaacg tggtcaccca cggcaacact caggagtaca gcgtcaaaat
     1561 tcaggtactg attctgatga ccctcgtcgg tttgctgccg acgatggtgc tgatgatgac
     1621 ctgtttcacg cgttttatta tcgtgctgtc actgctgcgc caggcgctgg ggctgcaaca
     1681 aacgccgcca aaccgcattc tgattggtat tgcgctctca ctcaccctgc tggtaatgcg
     1741 ccccatctgg ctcaatattt acgaccacgc ggtagtgccg ttcgaaaacg atcagattac
     1801 cctgaccgat gcgttaagca ccgccgcgac gccgctaaaa cgctttatgc tggcgcagac
     1861 cgacaaaaaa gcgatggcgc aaatcatgac catcggcggc gcgaagggca atgccgccga
     1921 ccaggatctc tccatcgtgg tgcccgccta tgtactgagc gagctgaaaa ccgccttcca
     1981 gattggcttt atgatttaca tcccgtttct ggttatcgac ctgattgtcg ccagcgtatt
     2041 gatggcgatg gggatgatga tgctgtcgcc gctgattgtt tcgctaccct ttaaactgat
     2101 gctgtttgtg ctgattgacg gctggtcgct gaccatcggc acgctcacca ccagcattcg
     2161 cgggctgggg ctgggctgat tatgttgacc gtagacgtcg ccgccgacat tgtcgccagc
     2221 ggcattaaag tagtgatttt gctggtttcg gtactggtgg tgccaagcct gctggtcggc
     2281 ctgctggtga gcgtgttcca ggcggtgacg cagattaacg aacagaccct gagctttttg
     2341 ccaaggctga ttgtcacgct ggtggtgctg ggcgtgtgcg gcaaatggat gattatccag
     2401 ttgcatgatc tctgcattca cctgtttacc caggccgcgt tgctggttca ttgagatgcg
     2461 cacaagcgac gttacgcaac tgatggatct ggcgctgggg ctgtggtttc ccttcgtgcg
     2521 cattatggcg tttctgcgct atatgccggt gctggataac agcgccctga ccgcgcgcgt
     2581 gcggattatt ttgtcgctgg cgctggcgat catcatcacg ccgttgatcc cccatcccat
     2641 tccccacaat ctgctatcgc tcaacagcct gatcctgacg gtggagcaaa ttttatgggg
     2701 catgttattc gggctgatgt ttcagttttt gtttctggcg ctacaactgg ccgggcaaat
     2761 tctctcgttc aatatgggga tgagcatggc ggtgatgaac gacccgagca gcggcgcatc
     2821 gaccaccgtg ctggcggagc taatcaacgt ctacgcgatc ctgctgtttt ttgccatgga
     2881 cgggcatctg ctgctggtaa gcgtgttgta caagggcttc acctactggc cgattggcaa
     2941 cgccctgcat ccgcaaaccc tgcgtaccat cgcgctggcc tttagctggg tgctggcgtc
     3001 ggcatcgctg ctggcgctgc cgaccacttt tatcatgctg attgtgcagg gctgctttgg
     3061 cttgctcaac cgtattgcgc cgccgctgaa ccttttttct cttggctttc cgatcaacat
     3121 gctggcgggg ctggtctgtt ttgccaccct gctctacaac ctgccggatc actatctgca
     3181 tctggcgaat tttgtcttac aacaactcga cgcgctgaaa ggtcactatg gcggatagca
     3241 gcagcgaaga aaaaacagag aaaccctcgg cgcaaaagct gcgtaaagcg cgtgaagagg
     3301 ggcaactgcc gcgctcgaag gatatggggc tggcggcgag cctgtttgcg gcgtttgtgg
     3361 tgatctccag cagttttccg tggtatgccg attttgtgcg cgagagcttt atcagcgtgc
     3421 atcagtatgc gcaggagatt aataacccgc aggtgatcgg ccagtttctg cgccatcacc
     3481 tgttaattct cggcaagttt atcctcacct tgctgccgat gcctgctgcc gcgctgctct
     3541 cttcgctggt gcccggcggc tggctgtttc tgccaaaaaa aatcctcccg gatttcagca
     3601 aaatcagtcc gttaaaaggc attgggcggc tgttttccag tgaacatctg gcggaaacgg
     3661 gaaagatgac ggtgaagtct gtggtggtgc tggtgatgct gtgggtaagc ctgcgcaata
     3721 actttgccgc ctttctcagc ctgcaggcgc tgccgttcaa actggcgata aatgacggtt
     3781 tatcgctcta cgccagcgtg atgcgcaact ttgtcattct gtttatcttc ttcgcgctta
     3841 tcgacgtgcc gctggcgaaa gccctgttca ccaaagggct gaagatgacc aaacaggagc
     3901 tgaaagaaga gtacaaaaat caggaaggta agccggaagt gaaggcgcgt gtgcggcgac
     3961 tgcaacggca actggcgatg gggcaaatcc gcaaagtggt gccgaaagcc aacgtggtga
     4021 tcaccaaccc gacccactac gccgtggcgt tacagtatga ccagtcgcgc gccgccgcgc
     4081 cgtttgtggt ggcaaaaggc accgatgaaa tcgccctcta tattcgccag gtcgccacgg
     4141 agaatcaggt ggaagtggtc gagttcccgc ggctggcacg ctcggtttac tacaccaccc
     4201 aggtgaacca gcaaattccg tttcagcttt accgggccat cgcccacgtt ctgacctacg
     4261 tcctgcaaat gaaacactgg cgcgagggcg cacagccgcg cccggccctg aacagacata
     4321 tttccattcc caaagaggtg cttaaactgg atggcgaaaa caactaaatc gttccttgcg
     4381 ctgctgcgcg gcggcaatct cggcgtgccg ctggtcatac tgtgtattct ggcgatggtt
     4441 attctgccgc tgccgcctgc gctgctggat attctgttca ccttcaacat tgtgctggcg
     4501 gtgatggtac tgctggtggc ggtgtcggcg aaacgaccgc tggaattcag cctgttcccg
     4561 accattttgc tgatcaccac cttaatgcgc ctgacgctta acgtcgcctc tacgcgcgtg
     4621 gtgctgttgc acgggcatct cggcgcgggc gcggcgggta aggtgataga gtcgtttggt
     4681 caggtggtga tcggcggcaa ctttgtcgtc ggtttcgtgg tgtttatcat cctgatgatc
     4741 atcaacttta tcgtcgtcac caaaggggcc gagcgtattt ccgaggtttc tgcccgcttt
     4801 accctggacg cgatgcccgg caaacagatg gcaattgacg ccgatcttaa tgccgggttg
     4861 atcaaccagg cacaggcgca gacccggcgt aaagatgttg ccagcgaggc cgatttttac
     4921 ggcgcgatgg acggggcatc gaagtttgtg cgcggggatg ccatcgccgg gatgatgatt
     4981 ctggcgatca acctgatcgg cggcgtctgt attgggatct tcaaatacaa cctgagcgcc
     5041 gatgctgcct tccagcaata tgtgttgatg accatcggcg acggcctggt ggcgcagatc
     5101 ccttccctgc tgctctccac cgcggcggcg attatcgtca cccgcgtcag cgacaacggc
     5161 gatatcgccc atgacgtgcg taatcagttg ctggcaagcc cgtcggttct ctacaccgcc
     5221 actggaatta tgttcgtgct ggcggtggtg ccgggaatgc cgcatttccc gttcctgatg
     5281 ttcagcgccc tgcttggttt taccggctgg cggatgagca agcgcccgca agcggcggag
     5341 gcggaagaga aaagcctcga aacgctaacc cgtacgatga cagaaaccag cgagcaacag
     5401 gtcagttggg aaactattcc gctgatcgaa cctatcagtt taagcctcgg ctacaaactg
     5461 gtggcgctgg tggataaagc gcagggtaat ccgctcaccc agcggattcg cggtgtacgg
     5521 caggtgatat ccgacggtaa cggagttctg ctgccggaga tccgtattcg ggaaaacttc
     5581 cgcctcaagc ccagccagta cgccattttc atcaacggca ttaaggctga tgaagcggat
     5641 attccggcgg ataaactgat ggccctgccc tccagcgaaa cctacggcga gattgacggc
     5701 gtactgggga atgacccggc gtacgggatg ccggtcacct ggattcaacc cgcgcagaaa
     5761 gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg ccagcgtgat tgccacccat
     5821 gtgaataaga ttgtgcgcag ctatattcct gacctcttta actatgacga tattacgcag
     5881 ctgcataacc gtttgtcgtc gatggcaccg cgactggcgg aagatttgag cgcggcactc
     5941 aattacagcc agttgctgaa agtgtaccgg gcgctactga ctgaaggcgt ttccctgcgc
     6001 gatatcgtca ccatcgccac cgtgctggtc gccagtagtg cggtgaccaa agatcatatt
     6061 ctgctggcgg cagatgtgcg cctggcgctg cggcgcagca ttacccatcc gttcgttcgc
     6121 aagcaggagc tgacggtgta tacgctgaat aatgagctgg aaaatctgct gaccaacgtg
     6181 gtgaatcagg cgcaacaggg cgggaaagtg atgcttgata gcgtgccagt ggacccgaat
     6241 atgctcaacc agttccagag cacgatgcct caggtgaagg agcagatgaa agcggcgggg
     6301 aaagatccgg tgctgctggt gccgccgcaa ctgcgcccct tgctggcgcg ctacgcaagg
     6361 ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag tgccggatga gctggagttg
     6421 aagataatgg gggcgttgat gtaggcatcc ggcatcggtg catattgccg gatgcggcgt
     6481 gaacgcctta tccggcctac gggtgagcac ggttttgtcg gcatgataag acgcgtagcg
     6541 gcgcatctgg caccggtgca caactgccgg atgcggcgtg aacgccttat ccggcctacg
     6601 ggtgagcgcg gttttgtcgg catgataaga cgcatctgcg tcgcatcagg catcggcgca
     6661 cagctataaa atgatacgct ccccagcact aaaatccgtt acgtcctccg cccaatcgat
     6721 gggatataac cctcgcgcga catcgcgatg aaacgttgag aatggccaat cactcacttg
     6781 ctttacccaa ccatgtttta ctggatttat ataaatataa tcaacatgat ggcgatagtc
     6841 ttgggtattg cgaatggcat gctcccaaaa acgcggttgc cagatatttt tcaatccaca
     6901 agcatgggta aattgctttt ttaatttccc gccagcgcga ggaaaaatca tcatcgcctt
     6961 caggtaatgt ccatatgcaa tgcatatgct ccggcaaaac tacccaagcg ttaatttcaa
     7021 aaggtctgtc tcgcttaacg ttaataatag cgttacggag cgtctgaaac tgggtggtca
     7081 aaagttggtt tcgacgattc tgtaaattta ccgtgaaaaa ccaggttccc cccttgatgt
     7141 aataacgacg atattcagac ataacgactc ctttttctca acggtaagaa gaaaaggaaa
     7201 agtcggcaat gatgttgtgt taaatcggga aagcggctcg caaaaaatgc acagatttat
     7261 aggcatgata agacgcgtag cgtcgcatca ggcaccggta cacaaccgcc ggatgcggcg
     7321 tgaacgcctt atccggccta caaaatcgtc tcttccgtca aaatccgccg cgcgcccaaa
     7381 aaatgatcct gccagaaagg ttcagctaac cgacttaccc gaatggtttc cccggtgcgc
     7441 ggtgactcga taaactgacc gtctccaaga tatacgccca tatggtcggc tatctcacgg
     7501 ctgtggatat ggaaaaacag caaatctccc cggcgcaggt cgttattcgc cacaatcgtt
     7561 gcccgacggt agtggtacat ctcattggcc gtgcgcggga gcttagcctc aaggatcttg
     7621 ttgtaggcat aaaaaaccaa cccgctacag tcgaaacctt tatcaggccg cgtgcccccc
     7681 cagacataag gcttgccaag ttgctgttcc aggcggtgaa tcgccacttc ggtgatgtta
     7741 tgcaggtggt tgctgctgag gaaatgattg ttttccgcca gcgcctgcca gcgtctgtcg
     7801 ttcgccttga gcggcgcagg aaaccattct ggatgctgtt ttatctgctc ccgattatgc
     7861 tggcgcagcg cccttctgct tttagcattg ccttccacaa tatagctggc ctgctttttc
     7921 agatgggttt ggtactgttt taataaacgc gcacggtttt gcatccgctg gcgggcggcg
     7981 tagctaaaag agatatgtga tgtggtggcg ctggcgagta aagggagcaa aacgcataac
     8041 gaaaacacgc ctggatgcag aaatctgccg tgcaagaacg aagacatgaa ggacataaac
     8101 cagctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt attaattgct
     8161 tatttgcata ttactattgc ggtttcataa tcttaggaaa taagaaataa atctacaatc
     8221 catgcgcttt tcggactctt gaaaactgca cctgtgctaa atttgtaaac cctgcgccga
     8281 caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga
     8341 tttgggattc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat
     8401 gcgtaaaatc gcattaattc ttgcgatgtt gttgatccca tgcgtttcgt ttgccggttt
     8461 gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg
     8521 atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatctct acgtcaaaat
     8581 gggcgagcaa tatcaactgc tcgacagcta taaaatctgt aaatattccg gcggcttagg
     8641 gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa
     8701 tcagttaaaa ccagacagcc gttactacaa agcgattaat attggattcc caaacgccta
     8761 tgaccgtgcg catggttatg aagggaaata cctgatgatt cacggcgatt gtgtttccat
     8821 cggctgctac gcgatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc
     8881 gctggtgttt ggtcagccga gcgtgcaagt gagtatttat ccgttccgca tgaccgacgc
     8941 caatatgaag cgccataaat attccaactt taaggacttc tgggagcaac tgaagccggg
     9001 ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca atggccgtta
     9061 cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca caactggcat caaactacac
     9121 gctccccgag gcaaaataag cgccattcac ctggcataat cttttgccag gtttcattgc
     9181 ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga
     9241 agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga
     9301 tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtcagaa agcagcatgt
     9361 tgaaaacgcc cttctgccgc agttcatccg ccagtgaggc gatatattta aacactgccg
     9421 ccatgttgcc cggtgtgcgc gggtaacgct gcgttaattt atgcaggagc cagcaaaagg
     9481 ctttttcgct atcggtttca ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc
     9541 ccgtcagttg tccattatgg gcataagtcc agttacgccc ccataactcg cgggtaaacg
     9601 ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg
     9661 aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt
     9721 gcggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt
     9781 tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg
     9841 tagggacgtt ggcactcatc ccgagcaatt cgcacatggg taacctccac aacagcggga
     9901 gcgcatggcc cccggcgaag attgatttaa gaattttcag gtcggatgtg gcgtgaacgc
     9961 attattcggc ctacggttcg ggtgcagatt gtaggcatga taagacgcgt cagcgtcgca
    10021 tcaggcatca gagcacaaat gccggatgcg gcgtaaacgc cttatccggc ctacgccaga
    10081 cttacttaac catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgga
    10141 tctcctgaat gcggtcggca taaccaaagt gcggtacgcg aatttcgata tccgccgtgc
    10201 cagccatttt gccgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg
    10261 ccgccgcgat cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta
    10321 catcgccttc gcgacctacc gcttcaacgt aacgggagaa aatatcattg aaaccaaaat
    10381 cgttaccgac gcaggaaata tgactaacgt cagaaatggc aatcgccggg tagcccggac
    10441 ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg catggcgtcg caatgggagc
    10501 cgccgttgcc acaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg
    10561 ccgcgcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg
    10621 ccgcttcgtt cagttcatta cgaataagat cctggtacat gaggatatcc ttcagcataa
    10681 atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg
    10741 ctatgaccag gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt
    10801 tgtaattata ttgtaaacgc attgctaaat gtttttacat ccactacaac catatcatca
    10861 caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc
    10921 gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt
    10981 ctgattttgc tcgcctggac agccgccctc ggcgttgcgg gtctgtggtc ggcgtgggta
    11041 ctagtgcctc tggccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg
    11101 atttccgcac cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag
    11161 aaagaagcga ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg
    11221 gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt
    11281 ctcgacggtc cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag
    11341 ctggcggacc tgccgccgga gttgtgggcg taccttaaag agcatcgttt cttcgcgatg
    11401 atcatcaaaa aagagtacgg cgggctggag ttctcggctt atgcccagtc tcgtgtgctg
    11461 caaaaactct ccggcgtgag cgggatcctg gcaattaccg tcggcgtgcc gaactcatta
    11521 ggcccgggcg agctgctgca acattacggc actgacgagc agaaaaatca ctatctgccg
    11581 cgtctggcgc gtggtcagga gatcccttgc tttgcactga ccagccccga agcgggttcc
    11641 gatgcgggcg cgattccgga taccgggatt gtctgcatgg gcgaatggca gggccagcag
    11701 gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc
    11761 gtgctgggtc tggcgtttaa actctccgac ccggagaaat tactcggcgg tgcagaagat
    11821 ttaggtatta cctgtgcgct gatcccgacc accacgccgg gcgtggaaat tggtcgtcgc
    11881 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtgttcgtg
    11941 ccgatcgatt acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg
    12001 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
    12061 tcggtagcgc tggcaacggg tgcgtatgcg cacattcgcc gtcagttcaa aatttctatt
    12121 ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc ctacgtgatg
    12181 gatgctgcgg catcgctgat tacctacggc attatgctcg gtgaaaaacc cgccgtgctg
    12241 tcggctatcg ttaagtatca ctgtacccac cgcgggcagc aatcgattat cgacgcaatg
    12301 gatatcaccg ggggtaaagg cattatgctc gggcagagca acttcctggc ccgtgcttac
    12361 cagggggcac cgattgccat caccgttgaa ggggcaaata ttctgacccg tagcatgatg
    12421 atcttcggtc agggggcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
    12481 aagaacaatg acgtaaacgc gttcgataaa ctgctgttca aacatatcgg tcacgtcggt
    12541 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
    12601 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
    12661 ctgctttctg atgtctcgat ggcggtgctg ggcggcagcc tgaaacgtcg tgagcgcatc
    12721 tcagcccgtc tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt
    12781 tatgacgacg aaggccgtaa tgaagccgac ctgccgctgg tgcactgggg cgtacaggat
    12841 gcgctgtatc aggcagaaca ggcgatggac gatttactgc aaaacttccc gaaccgcgtg
    12901 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct
    12961 gacaagctgg atcataaggt ggcgaagatt ttacaagtgc cgaatgccac ccgttcacgt
    13021 attggtcgcg gtcagtacct gactccaagc gagcataacc cggttggttt actggaagag
    13081 gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt
    13141 aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg
    13201 attgataaag atgaagccgc tattctggtg aaagctgaag aaagccgtct gcgtagtatt
    13261 aacgttgatg actttgatcc agaagagctg gcgacgaagc cggtaaagtt gccggagaaa
    13321 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttttttatc
    13381 ggctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
    13441 gaacagcacc gttttgccat caatcgaaag cgcatcattc acattcagcc aggtgagttt
    13501 ctcttgcgac gttttctcat caatagtcga gaacagcccc gtcatctgat tagatttctc
    13561 ggaccacatc acagcgatac gttgcgagcc acagtcatga ggtttgcacg cgctcatcac
    13621 ctgatacgtc tcatctccca atgtcacggt ttgtgcggga gtataagtac cgcctttcat
    13681 cacccaggca ggcagcttat gcccttgcac catctgatta aacgccgctt tggtggtttc
    13741 gccctttgca agactgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
    13801 cagagcggcg actgtcgtta ttgccttaaa catcattcct cccgggctta tcctgcccat
    13861 gtgattaact ccctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
    13921 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttagagt
    13981 tataaaaccc ttcgcataaa ggagttgtac accgtgcctg gtttgaagat tacgcttttg
    14041 cagcaaccac tggtgtggat ggatggtcct gccaacctgc gccattttga tcgtcaactg
    14101 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
    14161 atggaagcgg cagcttcgtc gctagcacaa gatgacgtag tgaactggat gaccgctaaa
    14221 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctgattcg
    14281 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
    14341 ctgttccgca tggcagatga gcatctgcat tataaagcag gcaatgagcg agtgattgtg
    14401 gaatggcgcg gctggcgtat tttaccgctg gtgtgctacg acttacgttt tccggtgtgg
    14461 tcgcgcaatc tcaacgatta tgacctcgcc atctacgttg ccaactggcc tgcaccgcgc
    14521 tctctgcact ggcaggcatt actgacagcc cgcgcgattg agaatcaggc gtatgtagcg
    14581 ggatgtaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
    14641 attaatccac aaggcgagat tatcgctact gccgacgcgc atcaggcaac gcgcattgat
    14701 gcggagctat cgatgatggc attgcgggaa tatagagaaa agtttccggc atggcaggat
    14761 gcggatgagt tcacatttcg ttagagggtg aatatggcta cgagataagt ttcgtggcta
    14821 tttttctctg gaacttaaac gacgaaatcc cgaatcagta atgattcggg ctttctgaat
    14881 attggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa
    14941 ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata ttacggattg
    15001 cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca
    15061 agtcgctatt tttagcgcct tttacaggta tttatgcccg ccagaggcag gttccgccct
    15121 tcttctgcac cagatcaaga cgggcttcat gtgctgcaag ctcttcatct gtcgcaaaaa
    15181 caacgcgtaa cttacttgcc tgacgcacaa tgcgctgaat tgttgtttca ccttgttgct
    15241 gttgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccggtc atcgccagat
    15301 aaacttccgc aaggatctgg gcatcgagta atgccccatg cagcgttcgt ttactgttat
    15361 ctatttcgta gcgggcacat aacgcatcga ggctgttgcg tttaccggga aacattttcc
    15421 tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga atatcgcgct
    15481 taagcaacga aaactcgtag tccataaagc cgatatcaaa cgctgcgtta tggatcacca
    15541 actccgcgcc gcggatatag tccatgaact catcggctac ctcggcaaac gtgggcttat
    15601 cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc accagccgat
    15661 cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc acttcaacgg
    15721 caccaatctc aatgatcttg tggccttcat agtgcgcacc aatctggttc ataccggtgg
    15781 tttcagtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc atttatgtca
    15841 gacttgtcgt tttacagttc gattcaatta caggaagtct accagagatg cttaaacagg
    15901 tagaaatttt caccgatggt tcgtgtctgg gtaatcctgg acctgggggt tacggcgcta
    15961 ttttacgcta tcgcggacgc gagaaaacct ttagcgctgg ctacacccgc accaccaaca
    16021 accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa cattgcaaag
    16081 tcattttgag taccgacagc cagtatgtcc gccagggtat cacccagtgg atccataact
    16141 ggaaaaaacg tggctggaaa acagcagaca aaaaaccagt aaaaaatgtc gatctctggc
    16201 aacgtcttga tgctgctctg gggcagcatc aaatcaaatg ggaatgggtt aaaggccatg
    16261 ccggacaccc ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca atgaatccca
    16321 cactggaaga tacaggctat caagtcgaag tttaagcctg tggtttacga cattgccggg
    16381 tggcaccaac cgcctggcga attcgcggtt tattcttgct ctgtttcatc ggatttagcg
    16441 ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat
    16501 gcgcattcaa tagttttcct ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt
    16561 gtagcacttc aaaattcaac aaagagagcc agtccagctg ccgcatcaga gtaaacatcc
    16621 ggctgttata gggcgatgtt ttgcgcaata ccggcacaag tttgcgtaat cccattaaac
    16681 tgatgggatt gaagccacta atcaccagcc aaccatcatc aatcagtacc cgatcggctt
    16741 cacgcaataa acgatgcgga tcagtgcacc acggcaatgt atgtgccagt agacaaacat
    16801 caacggattt atcggcaaaa ggaagatgaa gtgggtccgc ctgcacctgg acgggcattc
    16861 cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc
    16921 ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac gggttgagct
    16981 gacgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag caatcaggag
    17041 ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc acactgagag
    17101 gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct gggttttgaa
    17161 tgatgaagca ggtcgctgtc tgattgtcga tcccggggac gcagagccag tattaaacgc
    17221 catttccgcc aataactggc aaccggaggc catatttctc acccaccatc accacgatca
    17281 cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt atggaccaca
    17341 agagacacaa gataagggaa caacacaggt agtcaaagat ggcgaaactg ccttcgtttt
    17401 ggggcatgaa tttagtgtaa ttgccacacc gggtcacact ttaggacata tctgttactt
    17461 cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg gtcggttgtt
    17521 tgaagggaca ccatcacaaa tgtatcaatc acttagaaag ttaagtgcgt tacctgacga
    17581 cacattggta tgttgtgctc atgaatatac cttatcaaat atgaagtttg ctttgagtat
    17641 tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa
    17701 aaatcaaata acactacccg taattctgaa aaatgagcgg caaattaatg tttttttaag
    17761 aacggaagat attgatttaa ttaatgtaat taatgaagaa acattattgc aacaacctga
    17821 agaacgtttt gcatggttaa ggtcaaagaa agataggttc tgataaaact ttcttgtcat
    17881 cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa ctattgacac acacatgaag
    17941 gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt gccagagcac cggcaacgtt
    18001 caacagcacg cacagagcct ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt
    18061 caggcacgat ggatggacga tgggacgtct atcgcgccag atggtgactt gtgggctttc
    18121 attggcgacg agctaaagat gggaattccg gaaaacgacc ggattcgcga acagaaacag
    18181 aaatatttac gcaataagag ctatctccac gatgtaactt tacgggcaga gccgtatatg
    18241 tactggatag ccgggcaagt taaaaaacgt aacatgccta tggaactggt actactaccc
    18301 atagtggaga gcgcttttga tcctcacgca acgtctggcg ccaatgccgc gggcatctgg
    18361 cagatcattc cgagcacggg gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg
    18421 cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca tgatgcagcg tctgaacaag
    18481 atgtttgacg gcgactggct tctgaccgta gcggcttata acagcggcga aggtcgggtc
    18541 atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg gtcgttaccg
    18601 ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg cattgagtga tattctcaaa
    18661 aacagcaagc gttatggcgt acgtctgcca acgaccgatg aaagccgtgc tctggcacgt
    18721 gtgcacctga gcagcccggt tgaaatggcg aaggttgcag atatggcggg gatttccgtc
    18781 agcaagctga agacattcaa cgcaggcgtg aaaggctcca cgctgggcgc aagtggtccg
    18841 cagtacgtga tggtgccaaa gaagcatgca gatcaactgc gtgaatctct ggcttcaggc
    18901 gaaattgctg ctgtacagtc gacgctggtt gccgacaata cgccacttaa cagccgtgtt
    18961 tacaccgtac gctctggcga cacgctttca agtatcgctt cacgtctcgg cgtaagcacc
    19021 aaagatttgc agcagtggaa caaactgcgc ggatctaagc tgaagccagg ccaaagtttg
    19081 acgattggtg caggcagtag cgcacagcga ctggcaaaca acagcgatag cattacgtat
    19141 cgtgtgcgca aaggcgattc gctttcaagc attgctaaac gccacggcgt gaacatcaaa
    19201 gatgtaatgc gctggaacag cgacactgcg aatctgcaac caggcgataa gctgacgttg
    19261 tttgtgaaaa acaacaacat gccagattcc tgacaaacca gataataaaa aaggcaccga
    19321 ttcccccggt gcctttttta tttatgccgc tttatgtgct tctagcatga tgatatcact
    19381 ggtgaaagag ccatcattct gcaaggcaaa atacgttttc acctctgtcg atgcgctctg
    19441 ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta cgcattctcg ctacccatga
    19501 agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat tggcttcact
    19561 gattaacgtc aaccactcac cgctggcgtg gttccgtatg tgagatgtat cgcgtaatgc
    19621 ttctaccgtc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta cgtccatcac
    19681 aatcagcaga ccaccgggtt tcaatacccg gttcacttca cgcaatgctg caccaacatc
    19741 atgccaatga tgggcagaat aacggctgat aacaatatca aatgcgttat cggcaaatgg
    19801 caggctttcg acataaccct ggcgggtggt gatatttttc agttgccggg cttcggcagc
    19861 ttgtgccacg acatccagca tatgggcaga taagtcatac gccaccaccg tgctcacgtt
    19921 ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta cacttgcatc
    19981 aggataatca gccagacgca ccgccagacg ctgtaaatct tgtccggatg catgtacggt
    20041 actggtcaga tattcgctgg cttgagagct aaactgcttt tctacgtggt catggtggga
    20101 ttgtgttgtc attgcgctgt ccttttgtta tttgagtata aaagggcagc gccaaacgtt
    20161 ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg actgaattca
    20221 acgagtagcg gattgtgatc ggacgcgcgc gtaaccagta cagaagcttc gctgacgttc
    20281 agaccacggt agaaaacaaa atcgagcggg cgaccaaacg cccgacggcg ctgatcatcg
    20341 gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc gttcatcctt
    20401 ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccactgtg gtgagctatc
    20461 tgatcgccaa taggaagtaa ctgcttacta tagacatcca cgcccagact gaagttgacg
    20521 gcgtgtatat taaccaccat caacaggcgg gtgtcaggta acggatagac cgtcaccagt
    20581 gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca atacactgga
    20641 tgtgccgccg aaagggtcat tacgccagaa ggatgttgtg gcagcacgaa agcgggtacc
    20701 tgatcggcgg caagatagtt agcagtcgca aactgtacta actctggcgt tgtctgcgct
    20761 tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga caaccattca
    20821 gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc ggtacttaac
    20881 ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg taagatcctt
    20941 tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac ttttgacttt
    21001 gaaacctcta tcacgtcaac ccttccgcac tcgggagggt tctactgtta tagggatttt
    21061 agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt acaccggtca
    21121 ggattttgct cattattgag aacattaaat cggatggcat atctcgcatc ctgatggttt
    21181 aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa atgaaagcca
    21241 cgtcggaaga actcgccatt tttgtttcag tcgtcgaaag cggcagcttt agccgggcag
    21301 cggaacaatt agggcaagca aactcagcgg taagccgggc ggtgaaaaag ctggagatga
    21361 aacttggcgt tagcctgctt aatcggacca cgcggcaact tagcctgacg gaagaaggcg
    21421 agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca gaatcagaaa
    21481 ttatggagac gcgtaataca ccgcgtggac tgttacggat cgatgccgca actccagtgg
    21541 tgctgcactt tctgatgccg ttgattaagc cttttcgtga acgctatccg gaagtcactt
    21601 tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg gatgtcgcga
    21661 tacgcgccgg tacgttaacg gattccagct tacgtgccag gccgctattt aacagttatc
    21721 gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa acgatcgacg
    21781 atttaaagca acatatttgc ctgggattca ctgaacccgc ttccctcaat acctggccga
    21841 tagcctgtag cgatggacaa ttacatgagg ttaagtacgg tttgtcatcc aatagtgggg
    21901 aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc gactacatga
    21961 tcgacaaaga aatcgctcgc ggagaattgg tggagttaat agcagataaa gtgttgccag
    22021 tagaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg cgcatccggg
    22081 cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct gtcagagagg
    22141 cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg caatccagtg
    22201 cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta agattactct
    22261 ccaggttttc acgtttagta gaagaaggaa ttactgagta accttccccc atagcccacg
    22321 ccagaatcac ttgagccgga gtcgcattgt gtttagctgc aatacgagca ataacctcat
    22381 ctttcagtgc cttgccatac gccagcgtca tataggaagt aatatggata ctgtgctgtt
    22441 tagcccaagc gaccacttta cggttttgca gataaggaga gagttcaatc tggttagtag
    22501 cgatgttttc agcgccaaca gcagcaatcg ctttttccat caatgggatc gtaaagttgg
    22561 aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc tgcataaact
    22621 cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga tcaacataat
    22681 cggtacgcaa tttttgcagg ctctctttca gactcgggat caatttgtct ttgctgagat
    22741 tttcaatcca gattttagtg gtgatgtgga gttcatgacg tggcacgcca ctttctgcaa
    22801 tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt gcgcgataat
    22861 caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga cggaaagtgc
    22921 ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt cgttaactga
    22981 ggtgattatg ggcttcagac tgttgcggaa aaatggcgta aaacgcagag gatttttgcg
    23041 attttggcaa taatagaaat acaaacgaaa aaaaacctcg agcgttaact cgaggcttct
    23101 tagtaagtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat
    23161 tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt ttatgaccgg
    23221 cttactgctt aatttgatac ctggcagttc cctactctcg catggggaga ccccacacta
    23281 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
    23341 cta
//
LOCUS       DAJOTB010000072        22957 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJOTB010000072 DAJOTB010000000
VERSION     DAJOTB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.072"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            94..1614
                     /locus_tag="OHN28_002707"
     CDS             94..1614
                     /locus_tag="OHN28_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCQ2298081.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEVLISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTNAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            1718..2653
                     /locus_tag="OHN28_002708"
     CDS             1718..2653
                     /locus_tag="OHN28_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2298082.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            2700..3320
                     /locus_tag="OHN28_002709"
     CDS             2700..3320
                     /locus_tag="OHN28_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HCQ2298083.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGGITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            3338..4867
                     /locus_tag="OHN28_002710"
     CDS             3338..4867
                     /locus_tag="OHN28_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298084.1"
                     /translation="MPPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNKVNVLI
                     GENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELNLFPNM
                     NVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQLVEIA
                     RALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELMEIGDFI
                     TIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTALHPSG
                     GYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETIDKCHFQ
                     ARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESASVKEM
                     ILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAKTDVYQ
                     LVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIAASTPQ
                     D"
     gene            4885..5952
                     /locus_tag="OHN28_002711"
     CDS             4885..5952
                     /locus_tag="OHN28_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2298085.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            6006..6803
                     /locus_tag="OHN28_002712"
     CDS             6006..6803
                     /locus_tag="OHN28_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2298086.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLAHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            6836..7621
                     /locus_tag="OHN28_002713"
     CDS             6836..7621
                     /locus_tag="OHN28_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2298087.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGDGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(7731..7883)
                     /gene="hokE"
                     /locus_tag="OHN28_002714"
     CDS             complement(7731..7883)
                     /gene="hokE"
                     /locus_tag="OHN28_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCQ2298088.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(8233..8385)
                     /gene="hokE"
                     /locus_tag="OHN28_002715"
     CDS             complement(8233..8385)
                     /gene="hokE"
                     /locus_tag="OHN28_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCQ2298089.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8828..9946
                     /locus_tag="OHN28_002716"
     CDS             8828..9946
                     /locus_tag="OHN28_002716"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2298090.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FISLSAASPYMQGTDTRFASSRPNIFSAFPDNGPMQWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            10012..10260
                     /locus_tag="OHN28_002717"
     CDS             10012..10260
                     /locus_tag="OHN28_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCQ2298091.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10325..10708
                     /locus_tag="OHN28_002718"
     CDS             10325..10708
                     /locus_tag="OHN28_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2298092.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            10787..11440
                     /gene="nfsB"
                     /locus_tag="OHN28_002719"
     CDS             10787..11440
                     /gene="nfsB"
                     /locus_tag="OHN28_002719"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCQ2298093.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            11548..12795
                     /gene="ybdG"
                     /locus_tag="OHN28_002720"
     CDS             11548..12795
                     /gene="ybdG"
                     /locus_tag="OHN28_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCQ2298094.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(12953..14335)
                     /gene="pheP"
                     /locus_tag="OHN28_002721"
     CDS             complement(12953..14335)
                     /gene="pheP"
                     /locus_tag="OHN28_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCQ2298095.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(14437..17580)
                     /gene="cusA"
                     /locus_tag="OHN28_002722"
     CDS             complement(14437..17580)
                     /gene="cusA"
                     /locus_tag="OHN28_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCQ2298096.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVSDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17592..18815)
                     /gene="cusB"
                     /locus_tag="OHN28_002723"
     CDS             complement(17592..18815)
                     /gene="cusB"
                     /locus_tag="OHN28_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCQ2298097.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEIDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(18831..19163)
                     /gene="cusF"
                     /locus_tag="OHN28_002724"
     CDS             complement(18831..19163)
                     /gene="cusF"
                     /locus_tag="OHN28_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCQ2298098.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19187..20569)
                     /gene="cusC"
                     /locus_tag="OHN28_002725"
     CDS             complement(19187..20569)
                     /gene="cusC"
                     /locus_tag="OHN28_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCQ2298099.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDITEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            20726..21409
                     /gene="cusR"
                     /locus_tag="OHN28_002726"
     CDS             20726..21409
                     /gene="cusR"
                     /locus_tag="OHN28_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCQ2298100.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21399..22841
                     /gene="cusS"
                     /locus_tag="OHN28_002727"
     CDS             21399..22841
                     /gene="cusS"
                     /locus_tag="OHN28_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCQ2298101.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
BASE COUNT         5688 a         5945 c         5762 g         5562 t
ORIGIN      
        1 gtcacagcag ggcctgatga agtaataacg tgaccatgag gaacgcctca tctccttcca
       61 cgccaggatg caatgtgact caggagacta cctatgtttg caggaaataa agagttcgtt
      121 attgcgttgg atgagggtac caccaatgca aaagccatcg cacttgatgg tttcggtaac
      181 gtggtggcga agttttctca accactggct attcagacgc cacgtgaagg ctgggttgag
      241 caatccgggg aagtgttaat ctctgcctcg cttgatgtta ttgcccaggc catcaaccac
      301 gtgggggcag aaaatgtagc ggctctggct atcagtaatc agcgtgaaac ggctatcggc
      361 tggtatcgtc agagtggaaa accactcaat gcggcgatta cctggcaatg cacgcgcagt
      421 gccgagtttt gtgaaacact gcgtcgcgag ggcaaagagc agcaaattaa aacgactacg
      481 ggtcttccta tcgccccgct attttccgcg tccaagatgc gttggctact ggaatctaca
      541 cctgagggtg tgcaacttgc cgaccagggt gaaatttgtc tcggcaccat tgatgcctgg
      601 ttgctatgga acttgactgc tggcgattca ttttgctgcg actactctaa cgccgcgcga
      661 actcaattac ttaacctgaa aacaggcgag tgggatgagg aaatgctggc gttgtttcac
      721 attccacgcg ctgcattgcc agaaattaaa ccttccagtg gcctgttcgg ttatacccga
      781 gggcttaaaa caattccgga tggtattccc gtcatggcga tggtgggcga ttctcacgcc
      841 gctctttttg gtcatgcgtt gggagaagcg ggatgtgtga aagcgaccta tgggaccggt
      901 tcttccgtca tggcccccgt caactctgct cagtgtgata tcgccacact tgccacaacc
      961 attgcctggc atgatggcga aaatattatt tacggtctgg aaggaaatat cccccacacc
     1021 ggcgatgccg tggcatggat ggctgacagc accggtttaa gtgaactgac aaacgcggag
     1081 ctggccctcg aactgaatac cctgccagcc tcagtggatt cgactatggg tgtttatttt
     1141 gttcctgctc tcactggcct gggcgcgcca tggtgggatg acagcgctcg cggcgtggtg
     1201 tgtggcttaa gccgtggcgt aaagcgcgcg catttgattc gtgccgcact ggaatcaatc
     1261 acttatcaaa ttgcagatgt agtggaggca atgcaacagc atgatgattt tcacttatcg
     1321 gcgttaatgg tggatggcgg cccgaccaaa aatgactggt tgatgcaata tcaggcggat
     1381 ctgctgggtt gcccggtgat gtgcagtgat gtgccagagc tttcagccat tggtgcgggc
     1441 ctgctcgccc gcaaagcgct tatccccggt accgttgcag atttaaaagc gttattaact
     1501 gaacacagtg aatttaaacc ggacatggcg cgtcatcagc gactgcaaaa gagatggcag
     1561 gagtggcgtc atgcggtaaa cagaacatta tggaaaccgg catcgtcaat ttgacgaaca
     1621 agactaacca acgtaaggag aataccggcc cataaataat tcaggcttta cgttgtttca
     1681 ccgtacccga caataacaac acagagagaa cattgtcatg aaattacgtt taacactgtt
     1741 aaccgctgcg accctgactg ctctttcttt ttctgctcat gcggtagaaa aaggaaccat
     1801 tatgattatg gtcaattcac tggataatcc ttattacgcc tcggaagcga aaggtgccag
     1861 tgaaaaagcc cagcaactgg gatataaaac gaccattctt tctcatggtg aagatgtcaa
     1921 aaaacagaat gaactgatcg atactgcaat tggtaaaaaa gttcagggca ttattttaga
     1981 taatgccgac tccactgcca gtgttgcagc cattgaaaaa gcaaaaaaag caggtattcc
     2041 cgttatatta attaatcgcg aaatacctgt ggatgatgtt gcactggtgc aaattaccca
     2101 taataacttc caggctggct cggaagttgc caacgcattt gtcgagaaaa tgggagagaa
     2161 aggaaaatat gccgaactca cctgtaatct tgctgataat aattgcgtaa ctcgctcgaa
     2221 atctttccac caggttattg accagttccc tgaaatggtc agcgtggcga agcaagatgc
     2281 caaaggcacg ttaattgatg gcaaacgaat aatggacagt attctgcaag cccatcccga
     2341 tgttaaaggg gtgatttgtg gtaatggtcc ggttgcactg ggagctatcg cagcactgaa
     2401 agccgccaat cgcaatgacg ttattgttgt cggcattgat ggcagtaacg atgagcgcga
     2461 tgccgtgaaa gccgaatcat tgcaggctac cgtgatgtta caagcacaag ccattgccgc
     2521 gcagggtgtt accgatctgg ataattacct gcaaaaagga gttaaaccag ccaaacagcg
     2581 ggtgatgttc cgcggcattc tgataaccaa agataacgca gataaagtcc aggatttcaa
     2641 tatcaaatcc tgatcccagg tcgttgcgcc ctcacaatga gggcgcagag gagaatatta
     2701 tggataaaaa agtctggctg gcgctggcaa ttctgagcct gggtggatgt cgcatcgtct
     2761 cgcagcagga acttgccgat ctcaaatccc cgcctaatcc tcatatggca aatatcgatc
     2821 agacctggca gaaaaacatc gtgccgcagg tggtagaaaa tgcgcgtccg gttgcggagt
     2881 taatggcggc gctacaggca gaaaaagatg tcgatgctgc ctgtaaaacg ctgggctatc
     2941 gttctcagga agaaaacccg tgtattttct acgttaaggt cgaaggtggc atcaccaata
     3001 ttgatgcgaa atcccgtagc ggaaaaatga ccatcacaga tatcagcggc acaaatattg
     3061 tcgtgcagac ggggccaact cttcgcggca cgcttctgcg agatgcatat aaaggtgcaa
     3121 gctatgagca ttttaacgat caggttttat ttggtgaata tggtcgtgct atcaatgagc
     3181 aagccatcaa tatgattcag tcggcaaagc ttaaaaccgg ggaaacggtt caggtttatg
     3241 gcgtattttc tgcatgggat attccgcaaa ctatcccgga tataacgcct gcgaaaatag
     3301 ccagggcggg aggcgagtaa tatgttgccg caagaaattg cccccccccc agactgatga
     3361 atcacctgta atcatagaaa cgcatgactt atcgcgtatt tatccgggtg tggtggcgtt
     3421 agataacgtg aattaccgag tctatcgcaa taaagtaaat gtgcttattg gtgaaaacgg
     3481 tgcgggtaaa tccacaatga tgaaaatgct ggcgggggtt gaaacacctt cctcaggcaa
     3541 gatcattctt gatggtaaag aagtcaccct aaattctacc catcaggccg aaaaacatgg
     3601 aattagtatc atttttcagg agctgaattt attcccgaat atgaatgtca tggataatat
     3661 tttcatggcg aatgaatttt ttcagaaagg gaagattaac gaaaaatatc aatatgaatt
     3721 agccaaatct ctattagaaa gactggaact gaatgttgat ccctataccc aactgggaga
     3781 gctaggtatt ggccatcagc aactggtgga gattgcccgt gccctttcta aagatacccg
     3841 ggtactgatt atggatgaac cgacatcggc cctcagccag tcggaagtaa aagtcctttt
     3901 caatgtgatc gcacaactga agcgccgtgg tgtcaccatc atttatatct cccatcgtct
     3961 ggaagagttg atggagattg gcgatttcat caccattttc cgtgatggtc gctttattag
     4021 tgaacgcgcc gtgagcgatg ccagtattcc atggatcatc gagcaaatgg tcggtgataa
     4081 gaaaaaacat ttcgattatc aaccagctac acaaggtgac actgtactgg cggtacaagg
     4141 gttaacggcg ctgcatccca gcggtggtta caaactcaat gatgtgacat tcagcctgca
     4201 taaaggcgaa gttatcggca tttacggctt gctgggcgca ggacgtactg aactatttaa
     4261 gggattggtg ggcctgatgc cctgtcagag tgggaagatc cagcttaatg gtgaaaccat
     4321 cgacaaatgc catttccagg cgcggctcaa aaaggggctg gcgttggtgc cagaagatcg
     4381 ccagggcgaa ggcgtcgttc agatgatgtc gattcagtcc aatatgacgc tatccgattt
     4441 cagcctgcaa ggttttcgcc gtgcctggaa atggctgaat ccgcaaaaag agagcgccag
     4501 tgtgaaagag atgatcctgc aactggcgat caaagtcagc gatgccgaac tccctattac
     4561 ctcactcagc ggcggtaacc agcaaaaagt ggtacttggc aaagcgttga tgacccagcc
     4621 acaggtggtg tttcttgatg aacccacacg cggcattgat gttggcgcaa aaacagatgt
     4681 ctatcagctg gtgggaaaaa tggcacaaca ggggctggca gtcatgttct cttcttcaga
     4741 actcgatgaa gtcatggcac tggcagatcg cattctggtg atggctgatg gccgtattac
     4801 cgctgatctc ccccggggcg aagtcacccg agagaaactg attgcggctt caacacctca
     4861 agattaatca ggctggaatt tcttatgaat cagaaatata tgatctatat gtacctgttg
     4921 aaggccagaa cgtttatcgc tctgctatta gtgatcgcct tctttagcgt gatggtgcct
     4981 aacttcctga cagcctctaa cctgttgatc atgactcaac acgttgcgat taccgggctg
     5041 ctggcaatcg gaatgacgct ggtgatcctg accggcggca tcgatctttc cgtcggcgcg
     5101 gtagcgggga tctgcggaat ggttgccggg gctcttctaa caaacggcct gccattgtgg
     5161 agcggcaata ttattttctt caacgtgccg gaagtgatcc tctgcgtggc ggtatttggc
     5221 attttagtgg gactggtgaa tggcgcggta atcacccgct ttggtgtcgc gccctttatc
     5281 tgcaccctcg gtatgatgta cgtcgctcgt ggctctgccc tgctgtttaa tgacggcagc
     5341 acttatccca atctcaacgg catggaaacg ctgggcaaca cgggtttcgc cacccttggt
     5401 tctggcacgt ttttaggcat ttatctacca atctggctga tgattggctt tttgatttta
     5461 ggctactgga tcacgactaa aacgccgctc ggtcgttata tctatgccat tggtggcaat
     5521 gaatctgccg cgcgactggc aggcgtcccc atcgtaaaag ttaaagtgtt cgtctatgcg
     5581 ttctccgggt tatgcgccgc atttgtcggt ttgattgttg cctcgcagct gcaaactgct
     5641 cacccaatga ccggcaatat gttcgaaatg gatgctattg gcgccacggt tcttggtgga
     5701 actgcactgg cgggtggtcg gggacgtgtc tctggctcga ttatcggtgc ctttgtcatt
     5761 gtcttcctcg ccgatggcat ggtgatgatg ggtgtcagtg acttctggca aatggtcatt
     5821 aaaggcctcg ttatcgtcac cgccgtggtg gtcgatcagt tccagcaaaa attgcaaaac
     5881 aaagtcattt tgatgcgtcg ccatgaagaa aagatggccg ccgcacctgc cgtaaaaccg
     5941 ctttccagtt aattaacatc attccaccgg gcattaaatt gcccggctta tacaggggaa
     6001 aaaatatgca acgtaatttt cagggtaaga ctgtagttat taccggggca tgccggggaa
     6061 ttggggccgg gatcgctgaa cgcttcgccc gcgatggcgc aaatctggtg atggtttcta
     6121 acgctgaacg cgtacatgaa actgcagagc aactgcgcca atgttatcag gctgacattt
     6181 tgtcattaga agtcgatgtg acagatgaag cgcaagtaca ggcactctat gaacaagcgg
     6241 ctgcacgttt tggcagcatc gatgtttcca ttcagaatgc aggcattatc accatcgatt
     6301 attttgatcg aatgccggct gctgatttcg aaaaagtgct ggcggtgaac actacggcag
     6361 tctggctctg ctgccgggaa gcagccaaat atatggtgaa acagaatcac ggctgcctga
     6421 tcaacacctc ttctggccag ggacgtcagg ggtttatcta taccccacac tatgcagcca
     6481 gcaaaatggg tgtgatcggc attacgcaaa gcctggcgca cgagttagcg cagtggaata
     6541 tcactgttaa cgccttctgc ccgggtatta tcgaaagcga aatgtgggac tacaacgacc
     6601 gcgtatgggg agaaattctc agtaccgatg acaagcgtta tggcaaaggc gagcttatgg
     6661 cggaatgggt ggaaggtatt ccgatgaagc gtgccggtaa acctgaagat gttgccggac
     6721 tggttgcctt cctcgcctct gacgatgcgc gttatctcac cggtcaaacc atcaacatcg
     6781 acggcggcct gattatgtcg taatgcccct gcgggctact aagagagata tcaaaatgat
     6841 cgcaaaagat tttgctcaac aattgtttga tctccacgat cgcgtcgcct ttgtcactgg
     6901 cgcaggtagc ggtatcggac aaatgatcgc tctggcttta gccagcgcag gcgcacgagt
     6961 ggtgtgtttt gatctacggg aagatggcgg actcaaagag acggttaatc atatcgaagc
     7021 acttggcgga caagccttgt attacacggg cgatgttcgg gaattgagag atcttcgcgc
     7081 agcagtcgct ctggcaaaaa cacattttgg tcgtctggat attgccgtaa acgccgctgg
     7141 aatcgctaac gcaaacccgg cgctggagat ggaaagcgag caatggcaac gcgttatcga
     7201 tatcaacctg actggtgtgt ggaattcctg taaggcggaa gctgagttaa tggtagagaa
     7261 cggagacggt tcgattataa atattgcgtc aatgtccggg attattgtca atcgtggtct
     7321 ggaacaggcc cactacaaca gttccaaagc cggcgtgatt catttatcta aaagtcttgc
     7381 gatggaatgg gtgagtaagg ggatccgcgt gaactcaatc agcccaggat ataccgcgac
     7441 accaatgaat acacgacctg aaatggtaca tcagactcgg gagtttgaaa gccagacgcc
     7501 gattcaacgg atggcgaaag tggaagagat ggcgggtcca gcgctgttcc ttgccagcga
     7561 tgcggcgtcg ttctgcacgg gtgtggatct ggtggtcgat ggtggctttg tctgctggta
     7621 aaaatggttt cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc aggtgcctta
     7681 ccaccctgac ctgacaaccg gatatgcggg gattgctccc cgcacaacgg ctacttcttc
     7741 ggttcgtaag cgagaacagc cttaaactca atattacgtt cctttacggt aaactcacac
     7801 agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac tatgactgcc
     7861 acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc
     7921 caaattgtct ggtttggagt ggcagcccca gggttgatag aaataactcc tggggcttca
     7981 tctttccaga cctcaaagtt aacctgaaac cggaaagctt caggcacccg ccgttatctt
     8041 acccttcctc aacaaaatag ctgtttattt atacagtaag tcctgtttcg gacttctgtt
     8101 ggcaaaactg atcattgccc ttaaaacgac atcgcccgca gccatcactg accgcgggcg
     8161 aaagtgtaaa gcaggtgcct taccaccctg acctgacaac cggatatgcg gggattactc
     8221 cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact caatattacg
     8281 ttccttcacc gtaaactcac acagcgagtc tccgaccaga agcgtaaatc ccagcaccgt
     8341 caaacacagc actatgactg ccgcaagggc atatttcgtc agcatgttta tattgcctcc
     8401 atgcgaagaa gggactacca tccaaattgt tcaggtttgg gtaggtagcc ccagggttga
     8461 tagaaatatc gtcctggggc ttcatctttc cagacctcag agtaaacctg aaaccggaaa
     8521 gcttcaggca cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag
     8581 taaatcctgt ttcggacttc tcactcccca tttaatcaac tgaagctgaa cccgtttttt
     8641 tctgcatgcc tcaatcgtca aaattcttcg ccgccagcca gctctgccat aacttgtcac
     8701 attgcaaaat gtagccaatt tgcttcagtc accatcacca aaaaaagaaa cattccagaa
     8761 cttccgcatc tcgcccagcc gctcccactt ctatactgaa tagaaaacgc caacataaga
     8821 gaaacctatg ccattacccg attttcatgt ttctgaacct tttaccctcg gtattgaact
     8881 ggaaatgcag gtggttaatc cgccgggcta tgacttaagc caggactcat caatgctgat
     8941 tgacacggtt aaaaatcaga tcacggccgg agaggtaaag cacgatatca ccgaaagtat
     9001 gctggagctg gcgacggatg tttgccgtga tatcaaccag gctgccgggc aattttcagc
     9061 gatgcagaaa gtcgtattgc aggcagcggc agaccatcat ctggaaattt gcggcggtgg
     9121 cacgcatccg tttcagaaat ggcagcgtca ggaggtatgc gataacgaac gctatcaacg
     9181 aacgctggaa aactttggct atctcatcca gcaggcgacc gtttttggtc agcatgtcca
     9241 tgttggctgc gccagtggcg atgacgccat ttatttgctg cacggcttgt cacggtttgt
     9301 gccgcacttt atctcccttt ccgccgcgtc gccatatatg caaggaacgg atacgcgttt
     9361 tgcctcttca cgaccgaata ttttttccgc ctttcccgat aatggcccga tgcagtgggt
     9421 cagtaactgg caacaatttg aagccctgtt tcgctgcctg agttacacca cgatgatcga
     9481 cagcattaaa gatctgcact gggatattcg ccccagcccc cattttggta cggtggaagt
     9541 tcgggtgatg gataccccgt taacccttag ccacgcggta aatatggcgg gattaattca
     9601 ggctaccgcc cactggttac tgacggaacg cccgtttaaa catcaggaga aagattacct
     9661 gctgtataaa ttcaaccgtt tccaggcctg tcgctatggg ctggaaggtg tcattaccga
     9721 tccgcatact ggcgatcgtc ggtcactaac ggaagccacc ttgcgtttac tggaaaaaat
     9781 cgccccttct gcacataaaa ttggcgcatc gagcgcaatt gaagcactgc accgtcaggt
     9841 cgtcagtggt ctgaatgaag cgcagctaat gcgtgatttc gtcgccgatg gtggctcgct
     9901 aattgggctg gtgaaaaagc attgtgagat ctgggccgga gaataagtcg ggaattgcct
     9961 tacagaagaa tatccctgac aatggtgttt taacttacat ttaacaatgt tatgaaacac
    10021 cctttagaaa ccttgaccac cgcagcaggc attttgctga tggctttcct ctcttgcctg
    10081 ctgctgcccg cccccgcact ggggctgacg ctggcacaaa aactggtgac cacgttccat
    10141 ctgatggatc ttagccagct ttacacgtta ttgttttgtc tgtggttttt agtgctgggc
    10201 gctattgagt attttgttct acgctttatc tggcgacgct ggttctcgct ggcggattaa
    10261 acgtcgatta tcgccgaact tcgcggcata ctttgcttat tctccttcgc ctaaaggaac
    10321 atttatggat aagcaatcac tgcacgaaac ggcgaaacgc ctggctcttg agttaccctt
    10381 tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt tttaaaattg gcggcaagat
    10441 ttttatgctg tcgtcggagc tgcgcggcgt cccctttatt aatctgaagt ccgatccaca
    10501 aaaatccctg ctaaatcagc aaatataccc aagcatcaag ccagggtatc acatgaataa
    10561 aaagcactgg atttcggtgt atcccggcga ggaaatctcc gaagcgttac ttcgcgatct
    10621 gatcaacgat tcgtggaatc tggtggttga tggtctggct aaacgcgatc aaaaacgact
    10681 gcgtcctgac tcaaccgtaa atcaatagcg catttttctc gcttaccatt tcatctcgat
    10741 ccttgtaatc tgctggcacg caaaattact ttcacatgga gtctttatgg atatcatttc
    10801 tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac ttaccccgga
    10861 acaggccgag cagatcaaaa ctctactgca atacagccca tccagcacca actcccagcc
    10921 gtggcatttt attgttgcca gcacggaaga aggtaaagcg cgtgttgcca aatccgctgc
    10981 cgataattac gtgttcaacg agcgtaaaat gcttgatgcc tcgcacgtcg tggtgttctg
    11041 tgcaaaaacc gcgatggacg atgcctggct gaagctggtt gttgaccagg aagatgccga
    11101 tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca agttcttcgc
    11161 cgatatgcac cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac aggtttatct
    11221 caacgtcggt aactttctgc tgggcgtggc ggcgctgggt ctggacgcgg tgcccatcga
    11281 aggttttgac gccgccatcc tcgatgaaga atttggtctg aaagagaaag gctacaccag
    11341 tctggtggtt gttccggtgg gtcatcacag cgttgaagat tttaacgcta cgctgccgaa
    11401 atctcgtctg ccgcaaaaca ttaccttaac cgaagtgtaa ttctcccttg ccgggcatct
    11461 gcccggctat ttcccctcag attctcctga tttgcataac cctgtttcag ccgtcatcat
    11521 aggctgctgt tgtataaagg agacgttatg caggatttaa tatcccaggt tgaagattta
    11581 gcgggtattg agattgatca caccacctcg atggtgatga ttttcggtat tatttttctg
    11641 accgccgtcg tggtgcatat ttttttgcac tgggtggtac tgcggacctt cgaaaaacgt
    11701 gccatcgcca gttcacggct ttggttgcaa atcattaccc agaataaact cttccaccgt
    11761 ttagctttta ccctgcaggg gattatcgtc aatattcagg cggtattctg gctacaaaaa
    11821 ggcaccgaag cggcagatat tctgactacc tgcgcgcagt tgtggatcat gatgtatgcg
    11881 ctgctttcag tcttctcgtt gctggatgtg attttgaatc tggcgcagaa attcccggcg
    11941 gcatctcagt taccgctgaa agggattttt caggggatta aactgatcgg cgcgattctg
    12001 gtcggcattt tgatgatctc gctgctgatt ggtcagtcgc cagcgattct gatcagtggt
    12061 cttggtgcaa tggctgccgt gctaatgttg gtatttaaag atccgattct tggtctggtg
    12121 gcaggtattc agctttccgc gaacgatatg ctgaaactgg gtgactggct ggagatgccg
    12181 aaatacggcg cggatggcgc ggtgatcgat attgggttaa ccaccgtcaa agtgcgtaac
    12241 tgggacaata ccattaccac tattcccacc tggtctctgg tttctgactc ctttaaaaac
    12301 tggagcggga tgtcagcttc tggcgggcga cgtattaaac gcagtatcag tattgatgtc
    12361 accagtattc gttttcttga tgaagacgaa atgcaacgcc tgaataaagc gcatttgtta
    12421 aagccttatt taaccagccg ccatcaggaa attaatgagt ggaatcgcca gcaaggttct
    12481 acggagtcgg tattaaacct gcgccgaatg accaatattg gaacctttcg tgcctatctg
    12541 aacgaatatc tgcggaatca tccgcggatt cgcaaagata tgaccttaat ggtacgccaa
    12601 ctggctcctg gtgataacgg tttaccgctt gagatctatg cgtttaccaa caccgtggtg
    12661 tggctggaat atgaaagcat tcaggctgat atattcgatc acatatttgc gattgtcgaa
    12721 gagtttggtc tgcgacttca tcagtcgcca accggcaatg atattcgctc tctggcgggt
    12781 gcatttaagc agtaattaaa aaaaccgctc tcatcgaatg gatgagagcg gctttcagat
    12841 tgttgacatc attttgtcgg atgcggcgtg aacgccttat ccgacctaca gaatcgttac
    12901 aaattgtagg cccgataagc ttgcgcatcg ggcaaatcag cctcacatca gcttaattca
    12961 ctttcctacg caatgtttta aacgccacaa acaggaacac aatccacacc ggcagcagaa
    13021 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
    13081 cgatgcaaag atagttgccg aacggataga gcagcgcctt aaattgtgtt tcacgcccct
    13141 gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc cagttcaaca
    13201 gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
    13261 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacgc
    13321 cgcgacggct gacgcgagtc aaaaactttg gcgcattacc ctgcacagaa aggccaaaca
    13381 gcatgcggct gttagagtaa accccgctgt tatacaccga cagcgatgcc accagaatga
    13441 cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg
    13501 ggctactatt ggatttcact tccacccacg ggtagagcgc cagtaaaacc accagtgaac
    13561 cgatataaaa cagcaggatg cgatacacca cctgattcac tgctttaggg atgctttttt
    13621 ccggatcgcg cgcttcagcc gcagtaatcc caatcagctc cagaccgccg aaggagaaca
    13681 taattaccgc cagcgacaaa atcagtccat tccagccggt ggcaaagaaa ccgccgtagc
    13741 gccagaggtt atcgatactg gctttctcac cgccgtgccc agaaaacagc agccacaggc
    13801 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt
    13861 cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
    13921 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
    13981 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
    14041 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
    14101 ccaccatctc gccgagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
    14161 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
    14221 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
    14281 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
    14341 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
    14401 gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg
    14461 cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag gcgcggtgat
    14521 catgccgcca atcatcggcg cggcaatccg gctcatcacc tctgaccctg ctcctgttcc
    14581 ccacagaatc ggcagcagac cagcgataat caccgccacc gtcatcgctt ttgggcgcac
    14641 gcgcagtacc gcgccgtgat ataacgcctc atccagcttc tgctcgctga atgtttgcgg
    14701 attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc
    14761 aaattcggcg gcgaccccgg caagggcaat aaaaccggtg cccgtcgcca cggaaagatg
    14821 aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat
    14881 aatcagcaac gcttcgccca cccgacggaa cgccagatac aacagcacga agatgatcat
    14941 cagcgtcatc ggcaccatca gtttcagctt atggttggcg cgctccagta gctcgaactg
    15001 cccggagaat gccacgctgg tacccggttt taactgcact ttttcagcta tcgctttttg
    15061 cagatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt
    15121 cgggcgcgca ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac
    15181 gtccgccaga gtgatttgct gcttcatcgg cgtgaggatc ggcaactggc gcagtgcctg
    15241 cgggctatcg cgccagcttt gcggataacg cagattaatt ggataacgag caatcccttc
    15301 caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc
    15361 caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga tataacgccc
    15421 accttccaga cgctcagcaa gcgcagaagc cacgcctggc accgttcgcg ccacttcttc
    15481 aatttgctcc gccatcgtgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
    15541 agggctttta atgccagtcg agagcatatc gatacggtta cgaattggcg gcacccacag
    15601 attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccatcgt
    15661 catgccaggc cgccactgat cctgcggctt aagctggata gtcgtttcta ccatctccag
    15721 tggagctgaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
    15781 cggtacgctc ataatcagct tgtcgttttt ttgcagcatg ctcgccgcct ctgctgcgga
    15841 aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa
    15901 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa
    15961 cagcgtggtt ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa
    16021 aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc agtagcccat
    16081 caggatcggg atcactacga tcgccagtag cgccgcgccc gccatcgcat aggttttggt
    16141 gaacgccagc gggccaaaca gacgaccttc ctgcccttcc agggtgaaga tcgggataaa
    16201 cgacagcgtg ataatcagca gactgataaa cagcgccggc cccacttcaa cagatgcatc
    16261 ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
    16321 ctcttccagc cgtttatgcg cattctcgat catgacgata gcggcatcga ccatcgcccc
    16381 gacggcaatc gcaatgccac ccaacgacat aatattggcg ttcagtccct gaaagtgcat
    16441 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
    16501 cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt
    16561 gccgctgagg ttgtcaatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
    16621 ctccacgcct tccggcagac tgcttttcag cgtttccagt ttgtccttca cagcggcgat
    16681 cacttcacgg gcgtttttgc cggaacgcag gatcaccacc ccgcccgcca cttcgccttc
    16741 gccgttcagt tcggcaatgc cccggcgcat ctccgggcca acctggacct tcgcaacatc
    16801 acgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
    16861 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttctatcga
    16921 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacgtcgg cgagactgat
    16981 gccatactgc gccaggcgct gaggatcgct aaccacctga tactctttca ccacaccgcc
    17041 caccgacgcc acttccgcaa cgtcagggat ggtttttagc tcatatttga gaaaccagtc
    17101 ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc
    17161 atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc ccgcaggcag
    17221 cttaccctgt acctggttga ggtactccag cacccgcgag cgcgcccagt acggatcggt
    17281 gccatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac cgcgcacagt
    17341 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
    17401 gatttgcggt gcctgaccgg gatagctggt tttaataatc acctgcacat cggaaagatc
    17461 cggcagtgca tccactggcg tgttaatgat ggtccaggtg ccccagatac tcagaaacaa
    17521 cgcgcccatc agcaccagaa aacggttcgc caccgagcga cgaataatcc attcaatcat
    17581 tggctattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca
    17641 gaaatattgg cttcagaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc
    17701 gccagaccag agcgtaacgc ggtgacgcct tgcgatgcct ggaaaacggc aacgcgtttc
    17761 ggtacaaagc gcccgtcagc atcaacggta atcacccgct gttcgttgcc ggtatcaatc
    17821 agcgcctgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg caacccggcg
    17881 ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgattt ccagacgcaa ctgcagcgtg
    17941 cgggtcgcgg catccacgcc gggtagcagg gtccatttgc gaatggtgag tgttttatca
    18001 ggtcgcgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatggac
    18061 tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc gaccacgtta
    18121 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc
    18181 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatcagcc
    18241 tccggcattc ccgccagtcg cagtcgctca agaatgcctt cagtctgggt cgccgtaccg
    18301 ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg
    18361 tcgagaagcg gcgcgccctt ttgcacttta tcgcccacgg taagcggata caccttgtcg
    18421 ataaacccgg cggcgcgggc ctgcacaatg gcatactgat actcgttgta actgacattc
    18481 gccgggaaac tctgggaaaa agtcagcggc ccgcgcgtga cggtcgccgt tttcaccccc
    18541 agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca
    18601 tatttcggca ccagatccat atccataaac ggcgatttcc ctggtttatc gaaccgcgta
    18661 ttgggataca tcgggtcgta ccagaataag actttacgtt ctgcggtcga cgttttttct
    18721 gcgggcggtt cctcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg
    18781 atcatgctgc cgataataag cgcgattttt ttcatctcat taaacccgga ttactggctg
    18841 actttaatat cctgtaataa agaaagattg ccctgctgga caaaattaaa cgctacttta
    18901 tcgccggttt taatttcgct cattttcgtc tgcggggtga tggtaaagcg catagtcatc
    18961 tccggccagt tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga
    19021 tcaacacctt ttaccacgcc agtggcgcta ataacctgtg gttgtgcttc gctcatggtt
    19081 tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt
    19141 gcgacttgca gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac
    19201 cgccccctaa ggcggtatat aaagaaattt cgttaacctg acgggcataa ttcaggtcaa
    19261 gtaaagtttg tcgggttgca aataaagaac gttcagcatc cagcacttcc agataactga
    19321 ctgcgccgtg ctgatataat gcccgcgccc gttgcagggt aatttgcagc gacgcaagat
    19381 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    19441 cttctttaaa ggcgttctgg attttctgtt cataattcac caccgactgc tgctggcgaa
    19501 tttcggtgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    19561 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
    19621 cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa
    19681 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatcaggg cgctgcaata
    19741 agatttgcga cgacaagccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    19801 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    19861 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttctatc accccgcgag
    19921 cctgttccag cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat
    19981 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagtt
    20041 gctgattgaa atagctttgc gcgacattag aaaccaacag aatatgcacc gcgcgctgag
    20101 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    20161 aaaaatcgag atcaaagctg gcgttaaggc cagtcgataa ctcccgcgtc gtggctgaat
    20221 cgcctttaag attgccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    20281 cggcatcggt cagacgatat tgcgcccgcg cttcctgcac tttcagcgcc gccatgcgca
    20341 aatcccggtt attcaccaac gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa
    20401 aggtgcgcca gcccgcgttc tggtagttat ctgctgcgtt aaccaggccg ttctggctga
    20461 gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac
    20521 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    20581 ttctggtgat tttatgctgc caactttact cgccaggctc tgattttccg gtgacaggaa
    20641 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    20701 tgcccgggca acatgcggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac
    20761 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    20821 cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat
    20881 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaggg
    20941 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    21001 gttgggggcg gatgactatc tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt
    21061 gcgcactcta ctgcggcgcg gagcggcggt aattatcgaa agtcagtttc aggttgccga
    21121 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    21181 cagtaaagaa tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg
    21241 ctcgcttatc gcctcgcagg tatgggacat gaattttgac agcgatacca acgccattga
    21301 tgtggcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agttgattca
    21361 gaccgtgcgc ggcgtgggtt acatgctaga ggtgccggat ggtcagtaag ccatttcagc
    21421 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctcgccacc atcgcggcgt
    21481 ttttcgcctt tgcatggatc atgatccact cggtaaaagt gcattttgcc gagcaggata
    21541 ttaatgattt aaaggagatt agcgccaccc ttgaacgggt acttaatcac cctgacgaaa
    21601 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aatgtgttga
    21661 tctccctggc aaatagccac ggtaaaacgg tgtatcactc ccccggtgcg ccggatatcc
    21721 gcgagtttac gcgtgacgcc atacccgata aagacgcgca ggctggcgag gtgtatctcc
    21781 tttccggccc gacgatgatg atgccagggc acggtcacgg gcatatggaa cacagcaact
    21841 ggcggatgat taacttgccg gttggcccgc tggttgacgg caaaccgatt tatacgctct
    21901 acatcgcgct ttcgatcgat tttcatcttc attacatcaa tgatttgatg aataaactta
    21961 ttatgaccgc atcggtaatc agcatcctga tcgtctttat cgtgctgttg gcggtacata
    22021 aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc
    22081 tcgacgttcg ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt
    22141 tcaaccatat gatcgagcgt attgaggatg tctttacccg acagtccaat ttctcagcgg
    22201 atatcgccca cgaaattcgc acacctatta ctaatctcat tacgcaaacg gaaatcgccc
    22261 tcagccagtc gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagaac
    22321 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc
    22381 agctaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    22441 ttttcgaggc gttagcagaa gatcgcggcg tggagctgcg gtttgttggc gacgagtgtc
    22501 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc
    22561 tgcgttatac gccaaccgga gagacaattg ttgtgcgctg ccagacggtc gatcacctgg
    22621 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgaacactta ccgcgattgt
    22681 ttgaccgttt ctaccgcgtt gatccttccc gccagcgaaa aggcgaaggt agcggcattg
    22741 gcctggcgat agtgaaatcg attgttgtcg cgcataaagg cacagttgcg gtaacgtcag
    22801 atgctcgggg gacaaggttt gttatcatat tacccgctta atccttcagc aaacggcaac
    22861 ttttataacc agtgtaaaaa taacatgccg caatagtaat acctattaat gataaaaagc
    22921 tgtcacaatt cataaaaaac cttaatatac gccaccc
//
LOCUS       DAJOTB010000073        22798 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJOTB010000073 DAJOTB010000000
VERSION     DAJOTB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.073"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(4..80)
                     /locus_tag="OHN28_002728"
     tRNA            complement(4..80)
                     /locus_tag="OHN28_002728"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Met,seq:cat)
     gene            complement(114..190)
                     /locus_tag="OHN28_002729"
     tRNA            complement(114..190)
                     /locus_tag="OHN28_002729"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Met,seq:cat)
     gene            399..1496
                     /gene="mltA"
                     /locus_tag="OHN28_002730"
     CDS             399..1496
                     /gene="mltA"
                     /locus_tag="OHN28_002730"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCQ2298102.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1573..2379
                     /gene="tcdA"
                     /locus_tag="OHN28_002731"
     CDS             1573..2379
                     /gene="tcdA"
                     /locus_tag="OHN28_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCQ2298103.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2430..2873)
                     /gene="csdE"
                     /locus_tag="OHN28_002732"
     CDS             complement(2430..2873)
                     /gene="csdE"
                     /locus_tag="OHN28_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCQ2298104.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2873..4078)
                     /gene="csdA"
                     /locus_tag="OHN28_002733"
     CDS             complement(2873..4078)
                     /gene="csdA"
                     /locus_tag="OHN28_002733"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCQ2298105.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4270..4497
                     /locus_tag="OHN28_002734"
     CDS             4270..4497
                     /locus_tag="OHN28_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCQ2298106.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4848..5765
                     /gene="gcvA"
                     /locus_tag="OHN28_002735"
     CDS             4848..5765
                     /gene="gcvA"
                     /locus_tag="OHN28_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCQ2298107.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGENPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5784..6179
                     /locus_tag="OHN28_002736"
     CDS             5784..6179
                     /locus_tag="OHN28_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCQ2298108.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6172..7272
                     /gene="rlmM"
                     /locus_tag="OHN28_002737"
     CDS             6172..7272
                     /gene="rlmM"
                     /locus_tag="OHN28_002737"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCQ2298109.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7316..8047)
                     /gene="fucR"
                     /locus_tag="OHN28_002738"
     CDS             complement(7316..8047)
                     /gene="fucR"
                     /locus_tag="OHN28_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCQ2298110.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8105..8527)
                     /gene="fucU"
                     /locus_tag="OHN28_002739"
     CDS             complement(8105..8527)
                     /gene="fucU"
                     /locus_tag="OHN28_002739"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCQ2298111.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8529..9947)
                     /gene="fucK"
                     /locus_tag="OHN28_002740"
     CDS             complement(8529..9947)
                     /gene="fucK"
                     /locus_tag="OHN28_002740"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCQ2298112.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATSIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10056..11831)
                     /gene="fucI"
                     /locus_tag="OHN28_002741"
     CDS             complement(10056..11831)
                     /gene="fucI"
                     /locus_tag="OHN28_002741"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCQ2298113.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11864..13180)
                     /gene="fucP"
                     /locus_tag="OHN28_002742"
     CDS             complement(11864..13180)
                     /gene="fucP"
                     /locus_tag="OHN28_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCQ2298114.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13727..14374
                     /gene="fucA"
                     /locus_tag="OHN28_002743"
     CDS             13727..14374
                     /gene="fucA"
                     /locus_tag="OHN28_002743"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCQ2298115.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14402..15550
                     /gene="fucO"
                     /locus_tag="OHN28_002744"
     CDS             14402..15550
                     /gene="fucO"
                     /locus_tag="OHN28_002744"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCQ2298116.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15655..16410)
                     /gene="xni"
                     /locus_tag="OHN28_002745"
     CDS             complement(15655..16410)
                     /gene="xni"
                     /locus_tag="OHN28_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCQ2298117.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16522..17889)
                     /gene="sdaB"
                     /locus_tag="OHN28_002746"
     CDS             complement(16522..17889)
                     /gene="sdaB"
                     /locus_tag="OHN28_002746"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCQ2298118.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17947..19236)
                     /gene="sdaC"
                     /locus_tag="OHN28_002747"
     CDS             complement(17947..19236)
                     /gene="sdaC"
                     /locus_tag="OHN28_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCQ2298119.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19793..21157)
                     /gene="ppnN"
                     /locus_tag="OHN28_002748"
                     /gene_synonym="ygdH"
     CDS             complement(19793..21157)
                     /gene="ppnN"
                     /locus_tag="OHN28_002748"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCQ2298120.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21269..22117)
                     /gene="queF"
                     /locus_tag="OHN28_002749"
     CDS             complement(21269..22117)
                     /gene="queF"
                     /locus_tag="OHN28_002749"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCQ2298121.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQLAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22185..22730
                     /gene="syd"
                     /locus_tag="OHN28_002750"
     CDS             22185..22730
                     /gene="syd"
                     /locus_tag="OHN28_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCQ2298122.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
BASE COUNT         5650 a         5615 c         5969 g         5564 t
ORIGIN      
        1 gattggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta
       61 ccaggctgct ccaccccgcg tcaccgtact gctttacttc atcaaatttt aattggttgc
      121 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
      181 ccaccccgcg tccgtggatg cgcactatac tctgctcgcg ttttcatgca accttttttt
      241 taccccgatc acggatttgg catgattttg aacaaaaaca aatcactaga gcctattttt
      301 tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt aaacgcggtg gtttgttatc
      361 ttcgttgcgc cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt
      421 accttcttat gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc
      481 gcggacagca atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag
      541 atgccgttgg cgcgccgatt aatgccggtg attttgccga gcaaattaac catatccgta
      601 attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc
      661 tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg
      721 aaggtgtcga caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg
      781 cgcgccatac ccgtcagggc gagttccagt atccgattta ccgtatgccg ccaaaacgtg
      841 gtcgtctgcc gtctcgtgcg gagatctacg caggagcgct gagtgataaa tatattctcg
      901 cttacagtaa ctccctgatg gataacttca ttatggatgt gcagggtagc gggtatatcg
      961 actttggtga tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct
     1021 atcgcagcat tggtaaggtg ttgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga
     1081 tgcaggcgat tcgtcactgg ggcgaaacac acagtgaagc tgaggttcgc gagctgctgg
     1141 aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa
     1201 gtgcggtgcc gctggttggc cgtgcttctg ttgcctccga tcgttcaatt attccgccag
     1261 gtactacctt gctggcagaa gtgccgctgc tggataataa cggcaaattt aacggtcagt
     1321 acgaattgcg tctgatggtg gcgctggatg tcggtggcgc aatcaaaggc caacacttcg
     1381 atatctatca agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg
     1441 gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg
     1501 atattctgta tgccgttttt acggatatga cagggtgagg ggtaacctca ccctttttaa
     1561 ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca
     1621 gcgcgtctgt atggtgaaaa agcattgcaa ctgtttgctg acgcgcatat ttgtgtggtt
     1681 ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctag cgcgcacggg gattggtgca
     1741 atcacgctta tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct
     1801 ctgcgcgata acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt
     1861 aacccggagt gccgtgtaac ggtagtggat gacttcgtga cgccggataa cgtagcgcaa
     1921 tatatgaatg ccggttattc gtacgtgatt gatgctattg atagtgtacg gcccaaagcg
     1981 gcgctgattg cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgctggt
     2041 gggcagattg acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggacccg
     2101 ttggcggcga agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa
     2161 ggtaagctcg gcgtggattg cgtgttctct accgaagcgc tggtgtaccc gcagtcagac
     2221 ggcaccgtct gtgcgatgaa agccacggcg gaagggccaa agcggatgga ttgtgcctca
     2281 ggatttggcg cggcgacgat ggtgaccgcc acttttggct ttgttgcggt ttctcatgcg
     2341 ctgaagaaga tgatggcgaa agcggcgcgt caggggtaaa caatcaggtc ggatgcgacg
     2401 cgaacgtctt atccgacctg gttctgtgct taaacctgct tcgccgcagc gataatcgcc
     2461 tcgcttaacg cattcaaccc ctggctgcgt gaggcgctaa gttgcgcacg taatcccagc
     2521 tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg cagcggtttt cccctcaacg
     2581 gcagtcaaca acaccgccaa caggccgcgc acaatgcgcc cttcgctgtc gccaaagaaa
     2641 tgcatcttgc cgttttcagc cacggtatat cccagccaga cgcggttttc gcatccggca
     2701 atctctttag cctgcgcttt taactcctct ggcaaagccg gaagctgttt ccccagcatg
     2761 atcaactgcc gatatttatc ttcccattgc gtcaacggtg cgaaggtatt gcgtaacgtt
     2821 tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt gcgggtttgt cattaatcca
     2881 ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat
     2941 tatatggcgc aaatgaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct
     3001 gagcgcaatg ctgcccggcg cgcagggcaa taccgtactc cgccagcagt gtcaccatat
     3061 cgctgtgatg aactccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg
     3121 aacgaaagcc tggacgtttc gccagcgctt cttctgcaag cgttgctaag ctacggctcc
     3181 agctttcggc ttggttgata tcgtaatctg tcagccattc cagcgccgcg cttaatccta
     3241 tgacgccagc gacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga
     3301 aaccgtcaaa actcacttcg tgaaccattt tgccgccgcc cagccagggc gacatcgctt
     3361 ccagcagttc tggtttgcca tacagcacgc cgatgcctgt cggaccatac agtttgtgac
     3421 ctgaaaaagc atagaaatca atatccagtt gctgaacatc cgcggggaaa tgcaccgccc
     3481 cctgagcacc atcaaccatc accaccatcc cggctgaatg agcaaaagta atcgctctcg
     3541 ccagatccgg gcaaccgcca gtgacgttcg acatctggcc caacgccaga atccgactac
     3601 ggggagtaat cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca
     3661 atttcaccac tttggccccc gtttgttggg cgaccatcag ccaggggacg aggttggcgt
     3721 ggtgttctgc cacactgaca ataatttcat cgcccggttg cagacgcgga cgggcatagc
     3781 attgcgccac catgttgatg gattcagtgg tgccgcgcgt ccagacgata tttttgtcat
     3841 ccgatgcatt cagtaattgt gccacttttt cccgcgcagc ttcgtaacgc gcggtcaggc
     3901 gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc gctcaggcta taaaactgtc
     3961 gggaggcttc aaccacggct tcaggtttaa gcgcggtcgc ggcgctgtcg agatagacgc
     4021 ccgcatcctg tagcgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg
     4081 tactcctcgg cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc
     4141 taaccatcag aattatctgg atatcctggc gaagccgcgt cagtaacata tgctgaatag
     4201 tgttaggcgt taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag
     4261 gagaagatga tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg
     4321 agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct
     4381 gacggcaaac cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc
     4441 aacaaacagc agatcaaccg taccgacgtg aaagagatgg tcgaactgga tcagtaattc
     4501 gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac
     4561 agacagggta aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc
     4621 agaccaattg caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac
     4681 acgcattccg ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc
     4741 tggtagaagc tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac
     4801 agtgtaatct aattgttaaa ttcatttaac atcaaagttt aaaagccatg tctaaacgat
     4861 taccaccgct aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc
     4921 gcgcagcaga agagcttttt gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg
     4981 aggattttct ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg
     5041 aagggcaaag ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc
     5101 gtaaactcca ggcccgtagc gctaaggggg cgttgacggt cagtttactc cccagtttcg
     5161 ccattcattg gttggttccg cgactttcca gctttaattc agcttatccg ggaattgatg
     5221 ttcgaatcca ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga
     5281 tattttatgg tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt
     5341 tattgccggt gtgctcgccg ctactgctga ctggcgaaaa ccccttgaag accccggaag
     5401 atctggctaa acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc
     5461 gacagttggg gttaaatcat atcaacgttc agcaagggcc aatttttagc catagcgcca
     5521 tggtgctgca agcggctatc cacgggcagg gagtggcgct ggcaaataac gtgatggcgc
     5581 aatctgaaat tgaagccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa
     5641 acgcttttta tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc
     5701 gccaatggat cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac
     5761 aataatttac gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta
     5821 gcggcttcat ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg
     5881 gggccgttga gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg
     5941 cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta
     6001 gcgttttcct cgcgttaggc acggtgctgt tcagcggcag cctttattgc ctggcgctgt
     6061 cccatctgcg tttgtgggcg tttgttacac cggttggcgg cgtgagcttc cttgcgggtt
     6121 gggcgttaat gttagttggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag
     6181 gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa
     6241 gccggccagc gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtgatt
     6301 tatgaatgtt atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca
     6361 ttaatttttg cccgccagtg gtttgtagtg ggggaactcc tgcagcattt gccgccagaa
     6421 gatcgtatta cccccattgt tggcatgttg cagggtgtag tagagaaggg cggtgaactg
     6481 cgtgttgaag ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc
     6541 accgtgccgc tacgcgctgc cttgcgtgat gcgggggtgc tggcgaacta tgaaacgccg
     6601 aagcgtccgg ttgtgcatgt attctttatt gcaccaggct gttgctatac cggttactca
     6661 tacagcaaca ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagat
     6721 gcgccgagtc gttccacgct caaactggaa gaggcatttc atgtgtttat tcccgcggat
     6781 gagtgggatg aacgcctggc gaacgggatg tgggcggtgg atttgggcgc ttgcccaggc
     6841 ggctggacct accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg
     6901 atggcgcaaa gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa
     6961 ttccgtccga cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggcg
     7021 aaagttgcgg cattaatggc gcagtggctg gttaatggct ggtgccgtga aactatcttc
     7081 aatctcaaac tgccgatgaa aaaacgctac gaagaagtgt cacacaatct ggcgtatatt
     7141 caggcacagc ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac
     7201 gatcgcgaag aagtgacggt gcacgtccgc cgtatctggg ctgcggtggg tggtcgtcgc
     7261 gacgagcgat aacaggcaag gccggggcgt cgtctccggc ctgctaccct ttccgtcagg
     7321 ctgttaccaa agaagttgca acctggcgct catcagaaat aatgtgcgtg acctcatcca
     7381 gatgcccgat gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg
     7441 attgcgcggc acgttttaat aacattgatt tgtaatcagc gttgatggca ttggagtccc
     7501 acagtgcgcc gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg
     7561 atttaagttg ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc
     7621 cgccggaact gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc
     7681 tattggtgaa gacctggatg ttgatgtcag gcaactggcg tgccagatac cagcaggttg
     7741 aactggcgtc taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga
     7801 tatctgcttt atgcgcataa tggcttttca gccggatgtg aaagggatcg ccactgtctt
     7861 gattttgacg gtggatatat ttagcgcgcc catgattgcg cagaatttta ccctgcgtct
     7921 gtaattcatt gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag
     7981 cttccgtggt caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg
     8041 ctttcatatt tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccggtat
     8101 gagattacgg tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg
     8161 tgataacgat cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga
     8221 tgatgtcagg acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag
     8281 gatcgagagt gtcaccttct accgccgcca tcatcaccag cggtggtgca taactgtcca
     8341 gttcaaataa cgggataatc gcctggagca agtcgctcac caacaggcca tcagcgcgga
     8401 tcacctgcgg tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc
     8461 catgccccat ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt
     8521 tcagcatttc acacctcctc tataaattca ggttcagttt gcgggtagaa ataacggtac
     8581 tgataatgaa tctgcgcgcg ggcttcttcc gggctgttaa attcccctac gccataccaa
     8641 ccaaacagcg cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata
     8701 tcaagcatat tggctttaat ctgattccac aatgtgttgc gacttcctcc gccgactaac
     8761 aataattcag aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat
     8821 tgcgtggtta acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtt
     8881 acgccttgcc agccagcgtt ctggcacgac aataaatcac actgcatttt cactccatct
     8941 gcgccaggcg cgatcaggcg agcttcttca atcaacattt gccagggagt ttcagccgtc
     9001 cagaacagtt ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta
     9061 tacaaccctg cctggctatc cagttcgcag gtagaaccgg cgtactgact taacagcgaa
     9121 gtatcaacct gggcgctgcg gaccattaaa atttcccatg taccggaaga gagcacgggt
     9181 tcattttgtt cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc
     9241 accggtatgc caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc
     9301 tgttcacccg cttccaccag acgagggaag agtcggcgtg gaatactggt ggcttgtaaa
     9361 atttgcggac tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgta
     9421 atatcagtag tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag
     9481 gcgtgcgcgc gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac
     9541 gtattgaaac taaaggctcc gactccagaa atagcctgca actgctgtgc ggagatcaac
     9601 cgttcaatat tgtccattac cacggctgtt cgcggacatt tccagctaat gatcggatag
     9661 agcagattgc cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata
     9721 ccacggatgt ggcaatcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt
     9781 tgcaaaatgg cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg
     9841 gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg
     9901 acattggtcg cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca
     9961 atatagccgg ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc
    10021 cggagcgacc gggcatcaca tcagggagtc atgtattaac gcttgtacaa cgggccgtag
    10081 ttctggcaag cgcggtaatc ctggccttca atatccatgc cgtgcgcggc ccaggcagaa
    10141 ggacgataca ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag
    10201 gcaagagtga taaagtctgc gccaacgtgg ccgatggtca gaactccgtg gttagcgccc
    10261 cagttcgcca ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca
    10321 aaccaggtgg ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc
    10381 ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg gtcccaggcc tttgatgatg
    10441 ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg
    10501 ccgccacgga agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca
    10561 gcctcttgct gagagatttc ccagtgcggc ttcatcgtcg gtttaccttc gctgtcgcgt
    10621 tgtttacagg agccgtccag cgcagcagaa ccggagttga tcaaatggat gatgccgtgt
    10681 tctgccagcc cgtccagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag
    10741 gtacggacat cggcaaatac ctgagcggtg ccggtgagct ggtggcccat taacattgcc
    10801 acgccgttaa gactgtcgtt ttcggtcgcc acgacaaacg gtttgcgcac gccattccag
    10861 tcaaatgaac tgttgaggat cgcttcagcg gtatcgccat tgggatattg atcggtccag
    10921 tgacgttgcc cctggaagcc tgcagcgatg gcgttgtagc caagtgattc ttccacgcga
    10981 ccaatatcgg ccagtttgct attgccttgc atcatgtcgc ggatacacat cgccatcagt
    11041 aaactttcgc gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt
    11101 tcatcttcgc catagcggaa gtttttatca gcccaggcca gcgccatttc cagttcggct
    11161 tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact
    11221 ttcattccca gccaggattc aaagaagttg tgatcaacga tggaaccggc gatacccatc
    11281 gaaacgccgc ccagcgacag ataactttta cctttcatgc tggcgactgc taaaccggcg
    11341 cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc
    11401 tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga
    11461 gccgctgcca ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc
    11521 gggcgggttg gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta
    11581 atggtgaggc ctacattctg actgctgaat ttttcttcgc aagcagcggc ttcagccata
    11641 cccgcgatac aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt
    11701 ttctcggtca gcagtgcagc cgtggctttc gccatattca ttgtttgttc ttcaagcgac
    11761 tcacgaacac ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg
    11821 atttttttca ttactgattc ctcactttat tcggaaaata tgttcagtta gttgccgttt
    11881 gagaacggaa acgggcaaag ataaagatga ctgcgaagca gagtgccggg atcagttcgg
    11941 cagtggggat gttgcccgct gcgtcactga caaaccccat gaccggagtg acaataccgc
    12001 cgccaataat ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct
    12061 taatgcccag cgagaagatt gttgggtact gaatcgacat aaaggcactg cataaagtca
    12121 gggctattaa gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga
    12181 ttaatgcgta ggctgccagg actttgtgtg gcgcgaagcg actgatgagc caggtaccgg
    12241 tgaaacgacc aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg
    12301 ctgtcatacc tggaatttct tctacagcgt agcgaattaa atagctccag caggccgttt
    12361 gtgcgccaac gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcca
    12421 ggcgagaaag cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca
    12481 atgccgggaa tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga
    12541 tcatataagg tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg
    12601 gagacatttt atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagacttt
    12661 gcccaaagac aaccgcgata attgcgccaa atgagttaaa tgtttgcgca agatttaagc
    12721 ggaagtgacc actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca
    12781 gacaacctaa tccggctgca ataataaata ggccaactaa aaacaaggtg tagttcatta
    12841 tttctgcggc gggccagaat aatgcagcgc cgaaggcata taaaaacaat ccggtaataa
    12901 tccctgcttt ataactgagt tttttcatca atatcccggc agggattggg ataatgaaat
    12961 aaccaaagta aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct
    13021 gctggaattg aggcaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca
    13081 gtgagcacag cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta
    13141 ccgcacggta actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga
    13201 agcttaagca cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca
    13261 tatcagtgaa tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg
    13321 ctctgtaata tgacggcggt cacacttatt agccggaggg tgactttatg tgactaccat
    13381 cactttaata atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc
    13441 agatgaccgt attaattgtt tgtgccgaaa acgggcattc ctggcaggga ggagtagaaa
    13501 aatgaccgtt ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag
    13561 aacttgcagt tatttactgt gattagttga accaggtcac aaaatataag cttgtgaatt
    13621 tctttctggt taatcaggaa gaatatggtg agtaattaaa cggctaattc aatagtgtga
    13681 aaggaacaac attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa
    13741 acttgctcgt cagattattg acacttgcct ggaaatgact cgcctggggc tgaaccaggg
    13801 aacagcgggt aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggtat
    13861 tccatatgaa aaactgacgg agtcgcatat tgtctttatt gatggcaacg gcaaacatga
    13921 ggaaggaaaa ctcccctcaa gcgagtggcg tttccatatg gcggcctatc aaagcagacc
    13981 ggatgccaac gcggttgttc acaatcatgc cgttcattgt acggcagttt ccattcttaa
    14041 ccgaccgatc cccgcgattc actacatgat tgcggcggct ggcggtaatt ctattccttg
    14101 cgcgccttat gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa
    14161 aaatcgtaag gcaactttgt tacaacatca tgggcttatc gcttgtgagg cgaatctgga
    14221 gaaagcgtta tggctggcgc atgaagttga agtgctggca caactttacc tgacgaccct
    14281 ggcgattacg gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa
    14341 attcaaaacc tacgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat
    14401 gatggctaac agaatgattc tgaacgaaac ggcatggttt ggtcgaggtg ctgttggagc
    14461 tttaaccgat gaagtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac
    14521 gctggtgcaa tgcggcgtgg tggcgaaagt gaccgacaag atggatgccg caggactggc
    14581 atgggcgatt tacgacggcg tagtacctaa cccaacaatt actgtcgtca aagaagggct
    14641 cgatgtattc cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca
    14701 ggatacgtgt aaagcgattg gcattatcag caacaacccg gagtttgccg atgtgcgtag
    14761 cctggaaggg ctttccccga ccaataaacc cagtgtaccg attctggcaa tccccaccac
    14821 agcaggcact gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg
    14881 caagtttgtt tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat
    14941 gatggatggt atgcctccag cgctgaaagc cgcgacgggt gtcgatgcgc tcactcatgc
    15001 tattgagggg tatattaccc gtggcgcgtg ggcgctaact gatgcgctgc acattaaagc
    15061 gattgaaatc attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga
    15121 agaaatggcg ctcgggcagt atgttgcggg tatgggattc tcgaatgttg ggttagggtt
    15181 ggtgcatggt atggcgcatc cactgggcgc gttttacaac acgccacacg gtgttgcgaa
    15241 cgccatcctg ttaccgcatg tcatgcgcta taacgctgac tttaccgatg agaagtaccg
    15301 cgatatcgcg cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa
    15361 tgccgccgtt gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttacg
    15421 tgatgttggt gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt
    15481 ttgtaccggt ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac
    15541 cgcctggtaa atgtgctgat gtgataatgc cggatgcgac gctagccgcg tcttatccgg
    15601 catataagtg cattaaattc aatgtgttga taatcgtttg catccggcac gccgttacct
    15661 taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat
    15721 atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc
    15781 aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca gtagctgcgt
    15841 ggcgcttttt ggtccgatcc ccgcaacacc cggtaccttt gaactgctga tccccgcaag
    15901 cccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc
    15961 atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca
    16021 gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc
    16081 cagcgtggcg gctaagtcat cggcttcgtt gccgctggcg gaccagcacg ggacgccgcg
    16141 ttgctcaaag gcggcgcgta atgcaggcat ctcgttgtgc aactcttccg gcattggcgg
    16201 tcgacccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc
    16261 atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcgtg
    16321 ctggcaggtt tcgacacagg gcgaaccctg aacggcatga atgcgacgaa taagattcag
    16381 tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg
    16441 gtaacattcg atgccagaac aggctatggc tggatgagaa atcgggaaga ggcctcgcaa
    16501 aaagaggcct ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg
    16561 agaggtttcg cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tctcgataac
    16621 tttatcgagg cagacgcgcg gctcgctggt acggcgcagc gccatacgcg cggcgttcac
    16681 cgcttttact gccgcaatgg cgttacgctc gatgcacggt acctgcacct gtccagcaac
    16741 cgggtcacac gtcagaccga ggttatgctc catggcgatt tccgccgcga tgcacacctg
    16801 cgccgggctt gcgcctaaca gttctgccag accagctgcc gccatagagc acgccacgcc
    16861 gacttcaccc tggcagccga cttcggcacc agaaatcgag gcgttcatct tataaagaga
    16921 accaatggcg ctggctacca gcaggtaacg cgccagtgag ttagcgttca cttcgcggat
    16981 aaacttgtcg tagtacgcca gcacggccgg gataatcccg cacgcaccgt tagtcggcgc
    17041 agtcaccacg cgaccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat
    17101 ccagtcaact accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg
    17161 tagtgccgca gcacggcgtg gaacgcgcag tttgccaggt aacacgcctt cggtagaaat
    17221 accgcgttca ataccgccac gcatgacttc ccagacgttc gccaggtgct gttccagctc
    17281 ttctttgctg tgcagcgcca gctcgttttt catcatcagg ccagagagtg acagcccggt
    17341 ttcctgacaa tgtttttgta gatcggcagc ggaactgtac ggataaggaa cttcaaccgg
    17401 tgcgctatcc tgctgaccaa aatgctcttc atcaacgata aagccaccgc caatagagta
    17461 ataagtctgg ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc
    17521 atgcagagaa aggttgtcgg catggaagtt catgcactga tcaaccggga actccacttc
    17581 atgctgaccg tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg
    17641 gatggaatcg atatccacgg tatccggcag gttacccgcc agacccataa ttatggcgat
    17701 atctgtgtgg tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg
    17761 ggtcacgtct ttaagcaggt tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt
    17821 cattggtcca acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac
    17881 gctaatcata ggaaatacat cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga
    17941 aaggacttag ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac
    18001 aacgaatacg ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc
    18061 gtacatcggc atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat
    18121 acccaggatg ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc
    18181 agtgatacgg ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat
    18241 caccataccg ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat
    18301 agcgataatc gcgataatcg gagccatcca cgcgatgatc ggcgcgttaa agtggttagc
    18361 caggtaagac agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag
    18421 gctcagtacg cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa
    18481 tgccaggatc ttagagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac
    18541 ggcgaaagaa gagatgatcg gagagtggtt gaacgagaac accattaccg gaattgccag
    18601 ccacagggtc atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc
    18661 cagtgctgcg ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac
    18721 aaacgggaat accagaatac tcatcgcttt aacgatcatc tgctcaccga agcgaacgat
    18781 ggtcatcata cccacgatca ggatcagcga cagaatcgca cgtggcggtg gcgtcatacc
    18841 cagttggtga gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag
    18901 gatcgggtag atagcgaaga agtagagcag agtaatcagt ttacctgcgc caataccaaa
    18961 gtgttcttct acaacctcgg tgatgtcttc gcccgggttt ttaccagaca gtacgaagcg
    19021 agtcaggccg cggtgagcaa aaaacgtcat cgggaatgca aggatagcca tgatgatcag
    19081 cgggatcata ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc
    19141 cgtgccgtaa aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc
    19201 tttcgacgca atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa
    19261 taattcagct tttagccagt tcagcccggg aaggtgcctg accgtaaaaa tgaaatctgt
    19321 ctatcggcga tggcttaatc attttccacc gtaataattt gagggcggaa agatacacac
    19381 aagttatgta tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat
    19441 aatcacaacg ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc
    19501 cattaaacga catctgactt cttctggggc gcgcaatata tgtaggttga actggaaaaa
    19561 tcaatacttt tagttaatca tggcgataat ttctttttca tggcgataaa atgtggttta
    19621 tgaaaatagg gaaacgtttg cgtttcaaca taaattgtat gacatattaa ctgtggtgat
    19681 atttgtcgcg tgaggttggt aagaaacatt catataacaa atgaaatgag ttgatgagta
    19741 agtgaccaaa aacgataaaa cccggcaaca aacgtcaccg ggggagggga gattacgtgc
    19801 agatttcgta gcaagggatg taggctgagc ctggcaactt catacgatgc tgggcaacaa
    19861 aaccctgtag caggtcgtcc atacgacgca taatctcttt gtcgccgttg attttgtaag
    19921 gaccaaactc ttcaatggcg cgaataccga cttcttttac attacccgcc acaataccgg
    19981 agaacgcacg gcgcaggtcg gcagccagca cttcaacggg ttgatccggg taaagcttca
    20041 gattagccat attctcgtga gacggctcaa acggcatttg caaatctggc gcaatgcgca
    20101 ttgaccagtt aaagctgtaa gcgtcaccag tgtcgcggcg attttctttc accagcggca
    20161 tcgatttttt catctgacga gcgacttcag cggcgtcatc aatgatgatg cggtaatggc
    20221 ggcgcgcgtt ttcacccagc gtatgcacga caaactcgtc cagtacgcgg aagtagtcgg
    20281 cgctctcttt cgggccggtg aggatcaatg gtaaaacctg atctttgttg gccgggttca
    20341 ttaaaattcc cagcaaatac agcaactctt ctgctgtacc cacaccgcca gggaagataa
    20401 tgataccgtg agcgatacgg acaaacgctt ccagacgttt ttcgatatct ggcatgatga
    20461 tcaattcgtt gaccagcggg ttaggcggtt cagcggcgat aatcgacggc tctgtcatac
    20521 caataaagcg actgtctttg taacgctgct gcgcgtgtcc gaccgcagca cctttcatcg
    20581 gcgcttccat cgctcccgga ccacagccgg tgcagatatt cagttcacgc aggcccaact
    20641 gattcccgac gcgacgggca tacaaatact cgttttcgtt aattgagtga ccgccccagc
    20701 agaccaccat atttggcgct tcaccgacat gcagcgcgcg agcgttacgc aagatggaaa
    20761 agaccaggtt agtgatgtgg acggagttgt cgagattcag gttgggaaaa cgaacggtgt
    20821 tatgaatttg cccataaacg aagagaatgt cacgcagaac cgcgaacaag ttggcctgca
    20881 aagcgcgaat aattcgccca tcgacaaaag cctcttccgg gggatttatc agttccagct
    20941 ttacgccgcg ttcacgtcgc aggacgttaa tatcgaaatt ttcaaaacga gacagcaatt
    21001 ctttgctgtt atcggtcaaa ctaccggagt tcagtacggc aagtgaacag ttgcgaaaca
    21061 gttgatagag gtcgctgctg gcggtgcgtt taagcatatc cacttccagc tgcgacaaca
    21121 tatccatgga gccaagcggg ctaatatgtg taatcaagaa aaactcctta tgggacgaat
    21181 atcgtcattc cctttgatta caatagccct gcctgatgac ctttcacaac cttctgcgtg
    21241 aatccagcac gcaaaattga gaaaaaattt attgccgaac cagtcttgtg gtcgatggga
    21301 caaaatcgct attactgcgc cacgggttaa tgtccagacc gccacgacgg gtataacgtg
    21361 cgtaaacgct caatttctct ggctggcaga agcgtaacag gtcattaaag atgcgttcca
    21421 cgcactgttc gtggaactcg ttgtgatgac ggaaagagac caggtaacgc agcagttttt
    21481 ctctgtcaat ttggcgtcca cgatactgaa tttggatgga accccaatct ggttgatggg
    21541 tgatcaggca gtttgatttc agcaggtggc tgacaagcgt ctcttccact actttttcac
    21601 cgctggtggc attctccaga tagtcagtag tgaattcata gttatcgata gtgatatcct
    21661 ggtcatcaat gcaggtgcca ttgaaatgac ctatcggctg gccttccagt tcatcaagac
    21721 gatataacgc cacgctaacc ttaccctggg cgcaagtgct taagtcgcgc tctagcgtct
    21781 ggcgcacctc atcccagtta ttaaaacgcg tctggttaaa actgttgaga tagagcttaa
    21841 aactcttcga ctcaatcaga tttacgctgg tgtaatcaag ttcaacatga ccgacagcga
    21901 gttgcggcaa acctttcgca ttcagccagg aaagttcata cagcgtccag atatccgtac
    21961 cgtgaaaagg caggttatcc gctttcagac ccagcgggtc gcgattcagg ctgcgtggaa
    22021 ctccttgcag taggctggcg tcataggtat cccggtaatc ggttgatttt ccaagtgtca
    22081 ggcccgcaag tgcctgatgg tttgcataag aagacatgtt tcaccgtcat aaaccagata
    22141 aactaaacga caagtgtatc tggtttaagt gaagagagaa attagtggac gatttgaccg
    22201 cacgagccct gaaagatttt actgcacgct actgcgatgc gtggcatgaa gagcataaaa
    22261 gctggccgtt aagtgaggaa ctgtacggcg ttccttcacc gtgcattatt tcaactaccg
    22321 aagatgccgt atactggcaa ccccagccgt ttaccgggga acaaaatgta aacgcggttg
    22381 aacgcgcttt tgatattgtg atacaaccca cgattcatac cttttatacg actcagtttg
    22441 ccggggatat gcacgcgcag tttggcgata tcaaactgac attgctgcaa acctggagtg
    22501 aagacgactt ccgtcgggtg caggaaaatc tgattggtca tctggtgact cagaaaaggc
    22561 taaaactgcc gcccacgcta tttatcgcga cgctggaaga agagttagag gtcatctcgg
    22621 tgtgtaatct ctccggtgaa gtgtgcaaag agacgctggg aacacgtaaa cggacgcatt
    22681 tagcctcaaa tcttgcggaa ttccttaatc aacttaagcc tcttctgtaa tccaaatacc
    22741 ccatctgatt gtgagagatc tcttacagac tctgtaggag atcgccagga aataagcg
//
LOCUS       DAJOTB010000074        22592 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJOTB010000074 DAJOTB010000000
VERSION     DAJOTB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.074"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            112..363
                     /gene="yefM"
                     /locus_tag="OHN28_002751"
     CDS             112..363
                     /gene="yefM"
                     /locus_tag="OHN28_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCQ2298123.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            360..614
                     /gene="yoeB"
                     /locus_tag="OHN28_002752"
     CDS             360..614
                     /gene="yoeB"
                     /locus_tag="OHN28_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCQ2298124.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGK
                     PESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            697..1521
                     /locus_tag="OHN28_002753"
     CDS             697..1521
                     /locus_tag="OHN28_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2298125.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1567..2496
                     /locus_tag="OHN28_002754"
     CDS             1567..2496
                     /locus_tag="OHN28_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2298126.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            2711..2773
                     /gene="yoeI"
                     /locus_tag="OHN28_002755"
     CDS             2711..2773
                     /gene="yoeI"
                     /locus_tag="OHN28_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCQ2298127.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            2763..4121
                     /gene="plaP"
                     /locus_tag="OHN28_002756"
     CDS             2763..4121
                     /gene="plaP"
                     /locus_tag="OHN28_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCQ2298128.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4195..5622)
                     /gene="sbcB"
                     /locus_tag="OHN28_002757"
     CDS             complement(4195..5622)
                     /gene="sbcB"
                     /locus_tag="OHN28_002757"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCQ2298129.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDD
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            5831..6997
                     /gene="dacD"
                     /locus_tag="OHN28_002758"
     CDS             5831..6997
                     /gene="dacD"
                     /locus_tag="OHN28_002758"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCQ2298130.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPSQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQMALWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7116..7589
                     /gene="sbmC"
                     /locus_tag="OHN28_002759"
     CDS             7116..7589
                     /gene="sbmC"
                     /locus_tag="OHN28_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCQ2298131.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            7787..8845
                     /locus_tag="OHN28_002760"
     CDS             7787..8845
                     /locus_tag="OHN28_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2298132.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            9017..9346
                     /locus_tag="OHN28_002761"
     CDS             9017..9346
                     /locus_tag="OHN28_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCQ2298133.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9447..9629)
                     /locus_tag="OHN28_002762"
     CDS             complement(9447..9629)
                     /locus_tag="OHN28_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCQ2298134.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLWEAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            9683..9829
                     /locus_tag="OHN28_002763"
                     /pseudo
     CDS             9683..9829
                     /locus_tag="OHN28_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(10118..10231)
                     /locus_tag="OHN28_002764"
     CDS             complement(10118..10231)
                     /locus_tag="OHN28_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCQ2298135.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(10244..10438)
                     /locus_tag="OHN28_002765"
     CDS             complement(10244..10438)
                     /locus_tag="OHN28_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298136.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDGRDSTETPATYASPHQLL"
     gene            complement(10435..10809)
                     /gene="cbtA"
                     /locus_tag="OHN28_002766"
     CDS             complement(10435..10809)
                     /gene="cbtA"
                     /locus_tag="OHN28_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCQ2298137.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNSITLGKYPEAK"
     gene            complement(10898..11266)
                     /gene="cbtA"
                     /locus_tag="OHN28_002767"
     CDS             complement(10898..11266)
                     /gene="cbtA"
                     /locus_tag="OHN28_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCQ2298138.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLSSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLACEAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(11340..11561)
                     /locus_tag="OHN28_002768"
     CDS             complement(11340..11561)
                     /locus_tag="OHN28_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2298139.1"
                     /translation="MKIIPRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11624..12100)
                     /locus_tag="OHN28_002769"
     CDS             complement(11624..12100)
                     /locus_tag="OHN28_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2298140.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(12116..12595)
                     /locus_tag="OHN28_002770"
     CDS             complement(12116..12595)
                     /locus_tag="OHN28_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2298141.1"
                     /translation="MKIASQNTTALSSATAPGNDQSRLVATLIPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEQDEDDDETWVLFNAMN
                     GNRAEMSPEAAGIATCLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12858..13679)
                     /locus_tag="OHN28_002771"
     CDS             complement(12858..13679)
                     /locus_tag="OHN28_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2298142.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(13900..14310)
                     /locus_tag="OHN28_002772"
     CDS             complement(13900..14310)
                     /locus_tag="OHN28_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298143.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMSCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKAQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(14326..15003)
                     /locus_tag="OHN28_002773"
     CDS             complement(14326..15003)
                     /locus_tag="OHN28_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298144.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            complement(15139..16209)
                     /locus_tag="OHN28_002774"
     CDS             complement(15139..16209)
                     /locus_tag="OHN28_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCQ2298145.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLVHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            complement(16206..17111)
                     /locus_tag="OHN28_002775"
     CDS             complement(16206..17111)
                     /locus_tag="OHN28_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCQ2298146.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(17108..19492)
                     /locus_tag="OHN28_002776"
     CDS             complement(17108..19492)
                     /locus_tag="OHN28_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCQ2298147.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTGFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WECSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            complement(19710..20144)
                     /locus_tag="OHN28_002777"
     CDS             complement(19710..20144)
                     /locus_tag="OHN28_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298148.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPSIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTALPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            complement(20366..21466)
                     /locus_tag="OHN28_002778"
     CDS             complement(20366..21466)
                     /locus_tag="OHN28_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
                     /protein_id="HCQ2298149.1"
                     /translation="MFDVDDTAPDTPAPRELSQDVQALINNGLDFLDKAREELEASKP
                     KFSVVSFWTAVEILLKVPLAHEHWSLVCSPKKPIKKQAYLAGDFQSVTYEETRERLKD
                     VLERPLDRETDSAFDKVRKHRNRVVHFYHPTFTEAEQRQILKEQADAWFALNRLLRDE
                     WKVIFGVKHNWKLAFGETRLIRGNKFYAEVRFKQVKPELEQLSEKNIQIGNCNECHQH
                     ATVTGTETTGNENRKLEVTRCKVCTSAVRQITLVCPDCEIPQLLPEGDSDFECEHCDY
                     TSDRYKLLDEELFHSVDEQLLSVFPAGCTNCMTPESVCKFGDGYLCTQCFIYYTELHV
                     CGCCGHLSDSVPELSHIRGCEFCDGDQRYFDD"
     gene            21865..22467
                     /locus_tag="OHN28_002779"
     CDS             21865..22467
                     /locus_tag="OHN28_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2298150.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
BASE COUNT         5443 a         5631 c         5659 g         5859 t
ORIGIN      
        1 caaagtctga tttttcgtct cgctgacaat gtcttgatct acaaactaat taataaatag
       61 ttaattaaca cccatcattg tacaatgaac tgtacaaaag aggagattta catgcgtaca
      121 attagctaca gcgaagcgcg tcagaatttg tcggcaacca tgatgaaagc cgttgaagat
      181 catgccccga tcctcattac tcgtcagaat ggagaggctt gtgttctgat gtcactcgaa
      241 gaatacaact cgctggaaga gacggcttat ctactgcgtt cccccgctaa cgcccggaga
      301 ttgatggact caatcgatag cctgaaatca ggcaaaggaa cggaaaagga cattattgag
      361 tgaaactaat ctggtctgag gaatcatggg atgattatct gtactggcag gaaacagata
      421 agcgaattgt taaaaagatc aatgaaatta tcaaagatac ccgcagaaca ccatttgaag
      481 gtaaggggaa accagaatcc ctgaaacata atttgtcagg tttctggtcc cgacgcatta
      541 cagaggagca ccgtctggta tacgcggtta ctgatgattc actgctcatt gcagcatgtc
      601 gttatcatta ttgaacagat tcattcatag aatgaattgt cagttttgat acgctagcgt
      661 agccttatag atctaaggtg tatcaggaga taacggatga aaaaggtcgc aattgtcggg
      721 ttagggtggt taggtatgcc gctggcgatg tcactttcag cgcgaggctg gcaagtcacc
      781 gggagtaaaa ccacacaaga tggtgtcgaa gcggcccgaa tgagtggcat tgatagctat
      841 ctgcttcgca tggagcctga gttagtttgc gattctgacg atctggatgc cctgatggat
      901 gccgatgcgc tggtcattac gcttccggca cgtcgtagcg gccccggcga tgagttctat
      961 ttacaagcgg tacaagagtt agtggatagc gcgctggccc atcgtattcc ccgaattatt
     1021 tttaccagct caacatctgt ctatggcgac gcgcaaggca cggtgaaaga aactaccccg
     1081 cgtaatccgg taaccaacag tggacgagta ttagaagaac tcgaagactg gctgcacaat
     1141 ttacccggta cttcggtcga tattctgcgt cttgcggggc tggtcggacc gggacgtcat
     1201 cccggacgct tctttgccgg aaaaaccgcg cctgatggtg aacatggtgt taatttagtc
     1261 catttagaag atgttattgg cgctatcact ctgttgttac aggcacctaa aggcggacac
     1321 atctataata tatgtgcgcc agctcaccct gcgcgtaatg ttttctatcc gcagatggcc
     1381 cgtttactgg ggctggaacc tccgcagttc agaaatagtc tggacagcgg caaaggtaag
     1441 attattgatg gcagtcggat ttgtaatgaa ctgggatttg aataccagta tcccgatccg
     1501 ctggtaatgc cgctggagta aacgatcacg ccagcaggac gccgcatgca ctgcaagggg
     1561 gcgtgtatga aaccactgct ggatgtgttg atgatcctcg atgccttaga aaaagaaggc
     1621 agttttgcgg cggcgtcagc caaactctac aagacgccgt ccgctctgag ttacaccgtt
     1681 cacaagctgg aaagcgacct taatattcaa ttgcttgatc gtagcggaca ccgcgctaaa
     1741 ttcacccgta ccggaaaaat gttattagag aaagggcggg aagttctgca taccgtgcga
     1801 gaactggaaa agcaggcgat caaactgcat gaaggctggg aaaacgagct ggtgattggc
     1861 gtggacgaca cctttccttt ttctctgctt gcgcctctca ttgaagcttt ttatcaacat
     1921 catagcgtga cgcgcctgaa gtttatcaat ggcgtactcg gtggttcctg ggatgccctg
     1981 actcaggggc gagcggatat tatcgttggc gcgatgcatg agccaccatc ttccagtgag
     2041 tttggtttct cgcggttggg cgatcttgaa caagtctttg cagtcgcgcc tcatcatccg
     2101 ttagcccagg aagaagaacc gttaaaccgt cgtatcatta aacgatatcg ggcgattgtg
     2161 gtgggggaca ctgcgcaggc aggcgcttct acggcctcac aacttctcga cgaacaagaa
     2221 gccattaccg ttttcgattt taaaaccaag ctggagctgc aaattagcgg ccttggctgc
     2281 ggctatttac cacggtatct ggcgcaacgt tttctcgata gtggcgcgtt aatcgagaag
     2341 aaagtggtcg cccaaactct ctttgaaccc gtctggattg gctggaatga acagaccgca
     2401 gggcttgcca gcggctggtg gcgagatgaa attttagcaa atagtgcgat cgccggtgtt
     2461 tatgcaaaat ctgatgacgg aaaatcagcc atttaaagaa aaattattat gacaagcctc
     2521 tcattctctt gtcatttccc ccccatttag gcacaatgcg ccgctgtcaa aaaatgacta
     2581 aaaaccgacg tttaattagc gtcggttatt ttttgcttca aaccaatcat tcataccaag
     2641 aggccgggct tcgtaccgga tagatattta ctaaaaatcg acagttgttg tcgctgagga
     2701 atccaaaaaa atggggcaat tttttgctta cgcgacggtt atcaccgtaa aggagaatga
     2761 ccatgtcgca taacgttact ccaaacacct ctcgcgtgga attgcgtaaa acgcttacgt
     2821 tagttccggt tgtaatgatg ggtctagcct atatgcagcc gatgacgctg tttgatacat
     2881 ttggtatcgt ttcaggcctc acggatggtc atgtgccgac agcctatgcg ttcgcattga
     2941 ttgcgatcct atttacggct ctgagctacg ggaagctggt tcgccgctat ccttctgctg
     3001 gctctgcata cacttacgcc cagaaatcca ttagcccgac tgtcggcttt atggtgggtt
     3061 ggtcttctct gctcgactat ctgttcgcgc cgatgatcaa cattctgctg gcgaaaatct
     3121 attttgaagc tctggtgcct tccattccat cgtggatgtt tgtggtggcg ctggtggcct
     3181 ttatgaccgc ctttaacctg cgtagtctga aatccgtagc gaacttcaac actgtaatcg
     3241 tcgtgttgca gatagtgctg atcgcggtga ttctgggcat ggttgtttat ggcgtatttg
     3301 aaggtgaagg cgctggtacg ctggcgagca cgcgtccgtt ctggtctggc gatgcgcatg
     3361 ttatcccgat gattaccggg gcgacaatcc tgtgcttctc ctttaccggc tttgacggca
     3421 tcagcaacct gtcggaagaa accaaagatg cagagcgcgt gatcccgcgt gcgattttcc
     3481 tgaccgcgct gatcggcggc atgatcttca tcttcgcaac ttacttcctg cagctgtact
     3541 tcccggatat ctctcgcttt aaagatccgg atgcgtcaca gcctgaaatc atgctgtacg
     3601 ttgcgggtaa agcgttccag gttggcgcgc tgatcttctc caccattacc gtactggcgt
     3661 ccggtatggc ggcgcacgca ggcgttgcgc gtctgatgta cgtaatgggt cgtgacggcg
     3721 tgttcccgaa aagcttcttc ggttatgtac acccgaaatg gcgtactccg gcgatgaata
     3781 tcatcctggt tggcgcgatt gccctgctgg caatcaactt cgacctggta atggctacgg
     3841 cgctgattaa ctttggtgcg ttggtggcgt tcaccttcgt taacctgtcg gtcatctcgc
     3901 agttctggat ccgtgagaag cgtaacaaaa cgctgaaaga tcacttccag tatctgttcc
     3961 tgccgatgtg tggtgcgctg accgttggtg cgctgtgggt taacctggaa gaaagctcaa
     4021 tggttctggg tctgatctgg gctgctatcg gtctgattta cctggcttgc gtaaccaaga
     4081 gtttccgtaa cccggttccg cagtacgaag acgtagcgta atcttacaaa ccggatgaca
     4141 taatcatccg gtaaatctca aaaagccgga gtttgtcgct ccggcttttt tgtttcagac
     4201 gatctcttcc gcgtactgcc aaagcgcttt taacagcgcc actttctctt tgtcatcagc
     4261 atactgttgt gccagcatct gcaattcatc agcgtagccc tgcaaaaact ctggcgtgaa
     4321 gacctggcgg cggtgctcca gccagcgttg ctgctcggca taatccagcg tccccgggaa
     4381 gttgcgcgct cgataattga acaacagttt ttcaattcgt ttatcaacaa aagtgatatc
     4441 cagtgccggt aaattacgcg gctcggtttc cagcacaatt ttcattgctg cacgatcggc
     4501 gtcactgaaa aagccattat aaagctgtgc atccacgtta tctgaaggcg taaacggttc
     4561 ggcttccgcg aatatcgcca ccactttttc acgcacttgc ggattttcac gcagaatttt
     4621 taggttatcg aggcaatgct gacgattaat tcccagtcgg tcggcatctt ccgggcgtag
     4681 cgtattcgcc tgggccagta ccggacattt atttatatgc accagcttaa ccggaacggc
     4741 ggcgttatcg ccaagatcgg cttttgcggt atataaacgc tcgcgcaatg tgtcgctatc
     4801 cagctccagt aatggcgaaa tgtctcctgc caaatccacc ataattacgg cattgcgatt
     4861 ttcaggatgc cacgccagcg gtgccaccca gctggtattg ccacgccatg ccccaaacat
     4921 tccggaaacg tgaaccaggg gtttcatctg cggaacatca atcaacgcca tcagtttgtg
     4981 tttattacga tgggtaaaga gataatcaaa caggcgtggc tgacgcgttt ttaccagttt
     5041 cgccatcgca atagtggcgt acacatcagc catcgcatcg tgggcgttgc tatgttcaat
     5101 accattcgct ttggttaaat gctcaaggcg aaagctcggt agatcgtcat cattttcagg
     5161 ccagtttatt ccttccgggc gcagggcata acaggcacgc ataacatcca gtaaatccca
     5221 gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg tagaaattac gataaaaaac
     5281 gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag cccagaatac aggtcttcgg
     5341 tacggtaaaa agcgagtgaa tacgggcggc aaacgcggct tcgttttctc ctttcgcccg
     5401 tgcctcctgc ggggtaatac cggtaattaa tactgctcca ggctggggta aatagtcatc
     5461 cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca ttgaattcgt tatcagtgcg
     5521 aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc gttccaaagg tttcgtaatc
     5581 gtgaaacaaa aaggtagatt gttgcttacc gtcattcatc attaggttaa atccgttatt
     5641 tctgctgtct gccagagtat caaatatcac cgtgctaatc agctttagcg cgacaatttg
     5701 gcagcgagtg gcaacagttc atgtcagata aaaatgagat agtagtcaca ttttcttgca
     5761 ctttattcca gccagttcat aagtatttcc gtaaaaagaa cagctatttg aaacacctga
     5821 gggtttgctg ttgaaacgcc gtcttattat tgctgcttct ttgttcgttt ttaatttatc
     5881 gtctggtttt gcggcggaaa acattccttt ttcacctcaa cctccagaga ttcatgccgg
     5941 gtcctgggta ttgatggatt acaccaccgg tcagattctc accgcgggta atgagcatca
     6001 acagcgcaat cccgccagcc tgacaaagct gatgacgggc tatgtcgtgg atcgcgctat
     6061 cgatagtcat cgcattacgc cagacgatat tgtcaccgtg gggcgtgatg cgtgggcgaa
     6121 agataatccg gtgtttgtcg gttcttcact gatgtttttg aaagagggcg atcgcgtatc
     6181 ggtacgtgat ttaagccgtg gtttaattgt ggattccgga aatgacgctt gtgttgctct
     6241 ggctgattat attgccggtg ggcaacggca gtttgttgaa atgatgaaca actatgccga
     6301 gaagctgcat ctcaaggata cgcattttga aacagtgcat ggtctggatg cacctagcca
     6361 gcatagctcg gcttatgatt tagccgtgct ttctcgcgct atcatccacg gcgagcccga
     6421 gttttatcat atgtacagtg agaaaagcct cacctggaac ggtatcaccc agcaaaaccg
     6481 taacgggtta ttgtgggata aaaccatgaa tgttgacggc ctgaaaacgg gccatacttc
     6541 tggtgccggg tttaatctta ttgcttcggc tgtagacggg cagcgtcgcc tcattgcagt
     6601 ggtaatgggt gctgacagtg caaaaggccg tgaggaagag gcaagaaaat tactgcgttg
     6661 ggggcaacaa aactttacta cggtgcaaat tttgcaccgt gggaaaaagg tcggtacgga
     6721 acgcatctgg tatggcgata aagaaaatat cgccctggga acggaacaag agttctggat
     6781 ggtgctaccg aaagccgaaa ttccacatat caaagccaaa tatacccttg atggtaaaga
     6841 actcaccgcg ccaattagcg cccatcagcg ggtaggggaa attgaacttt acgaccgtga
     6901 taaacagatg gcgctctggc cgctggttac cctggaatct gtcggggaag gcagcatgtt
     6961 ttcccgcctg agtgattatt tccaccataa ggcctgacct ttcttttgca gcagactggc
     7021 aggagtgcga gtctgctcgc ataatcaaca ctcattcctt gtggttttaa tattgcaact
     7081 atactgtata taaaaacagt gttagtggag gcgtcatgaa ctacgagatt aagcaggaag
     7141 ataaacgtac cgttgcaggt ttccatcttg ttggcccgtg ggagcagacg gtaaagaaag
     7201 gttttgagca gttgatgatg tggatagata gcaaaaatat tgtgccgaag gagtgggttg
     7261 ctgtctatta cgacaatcca gatgaaacac ccgccgaaaa attacgctgc gacaccgtcg
     7321 tgacggtacc gaataacttt acgctccccg aaaacagtga gggcgtcatt ctgacagaaa
     7381 tttcaggtgg tcagtatgcg gtggcggtgg ctcgtgtagt cggtgatgat tttgctaaac
     7441 cctggtatca gttctttaat agcctcttgc aggacagtgc ttatgaaatg ttaccaaagc
     7501 cctgcttcga ggtttacttg aacaatggcg cggaagatgg gtactgggat atcgaaatgt
     7561 atgttgcggt gcagccaaaa catcactaat tcatctcagg gcggtgtgtt aacgcgaaga
     7621 ccactctttt tttgaaagcg gaaagagtaa gatgcgcctt tcaaattttt cgctcctgcc
     7681 gggaaattac actgttcccg gtatgtccgt cggataactc agaggcgcgc cttctggccg
     7741 acagatgtgt tatgagcgct tttaatcttc attacggagt ttctgcgtgc gtgccgataa
     7801 gtcattaagc ccgtttgaaa tccgggtata ccgccattac cgcattgtgc atggtactcg
     7861 ggtcgcgctg gcattcctgc tcacttttct cattatccgc ctgtttacta tcccggaaag
     7921 cacctggccg ctggtcacca tggtggtgat tatggggcca atctcgttct gggggaacgt
     7981 tgtccctcgt gccttcgaac gtattggcgg tacggtgttg gggtcgattt taggtcttat
     8041 cgctctgcaa ctggagttaa tttcgttacc gctgatgtta gtctggtgcg cggcggcgat
     8101 gtttctttgc ggttggctgg cgctgggcaa gaaaccgtat caaggattat tgattggggt
     8161 gacgctggca attgttgtgg gttccccgac aggtgaaatt gatacggcgt tatggcgaag
     8221 cggcgatgtg atcctcggct ctttactggc aatgttgttt accggtatct ggccacaacg
     8281 ggcgttcatc cactggcgca ttcaactggc gaaaagtctg accgagtata atcgggtcta
     8341 tcaatctgca ttctcaccga acttactcga acgcccacgt ctggaaagcc atctacaaaa
     8401 actcctgacc gatgccgtga aaatgcgtgg gctgattgcg cccgccagca aagaaacccg
     8461 tattcccaaa tcgatatatg aaggtatcca gaccattaat cgcaatctgg tttgtatgct
     8521 ggagttgcaa atcaatgcat actgggccac gcgccccagc catttcgtgt tattgaacgc
     8581 gcaaaaactt cgtgataccc agcacatgat gcagcaaata ctgctgagcc ttgttcatgc
     8641 gctgtacgaa ggtaatccgc agccggtttt tgccaatacg gaaaaattga acgatgctgt
     8701 ggaagagctg cgtcagttgc tcgataacca ccatgacctg aaagtcgtgg aaacaccaat
     8761 ctatggttat gtgtggctga acatggaaac ggcacatcag cttgagttgc tatcgaatct
     8821 gatttgccgg gccttgcgca aataattcct gaacttcaga atcaccttgc tgctgcttcg
     8881 attcagcaag gataaagggt atgatagtga aaagggataa aagcattgtc atctgcggca
     8941 gctatgagta atgttggccc taacgaatag cggttgctta aacgaatccg actctcacat
     9001 tatcaggggt ataaaaatgg aaactaccaa gccttcattc caggacgtac tggaatttgt
     9061 tcgtctgttc cgtcgtaaga acaaactgca acgtgaaatt caggacgttg agaaaaagat
     9121 ccgtgacaac cagaagcgcg tcctgttgct ggacaacctg agcgattaca tcaagccagg
     9181 gatgagcgtt gaagcaatcc agggcatcat cgccagcatg aaaggtgact atgaagatcg
     9241 cgttgacgat tacatcatca aaaatgccga gctctccaaa gaacgccgcg atatctccaa
     9301 aaagctgaaa gctatgggcg aaatgaaaaa cggcgaagcg aagtaattcc cattttattc
     9361 aatgagggtt gcccggcaac cctcattgct cattgattct tatctgtgta tcaccgtcat
     9421 cattctcatc tgagaaccaa tcgaaattaa caacagcctt cttctgtatg cagcaaggca
     9481 aaaagttctg taactccatt gttattaact gcactggtta ctaacacgtt gtgcgctcca
     9541 gcttcccata accaactttt caccaaagat atttgttcca tgctggctaa atctgctttg
     9601 gttactactc caatgaccgg gtgattcatg gcccggtagg gcgtttttac ctctaattcc
     9661 tgcttcagcg ctgagattcc tgcttgttgc cggaaaagca ggcacctgcc gtctgaacgg
     9721 tatcgatccg gaagcgtatc tgcgccatat tctgagcata ctgccggaat ggccctccaa
     9781 ccgtgttgac gaactcctgc catggaacgt agttctcacc aataaataag cgtcaatacg
     9841 gtgctccgtt gacgcttact ttaatccgtt ttctgagctt agatttttct gttcctaccc
     9901 agaccgtgga tttggaattc attttttgtc aggtcaatgc taatcgttaa aacggccata
     9961 acgcacctcc actggaaggt ttatcagtaa tatcctaccg ttgcttcatg gtcggggcgt
    10021 ccattccatt attatcggtt ttcctttctg tttataaatg aaaacgccag ctgtattcag
    10081 gctggcgtca ggggaaatga agcctgttga gtgagattca ccggttctgg tgcagaagct
    10141 gcagatggcg cagggtaagg acatcttctc ttgcctgctt aatacaggac aacagtgctg
    10201 tttctgtggt taatggcact ataccctgta tcactgcatt gtcttacagc aactggtgag
    10261 ggctggcata agttgccggt gtttcggtag agtcacggcc atccaccacg cggagtgaat
    10321 aaccgttttc agcggcctta ttaatcagtt cagtgatatt aacaccatca atgtcaacga
    10381 caatgtgccc catatccagc gcctgtacgt taacgctgtc ggcttccagt gtcatcattt
    10441 cgcctccgga tacttaccca gggtaatgct atttaccgtt ctgtaattgt cgcgggtcat
    10501 caggccggtc gccctgcgag cccggaggat atcgatgctg tttattaact gagagcgggt
    10561 acaggcgctg aatcccggct ggtcggtacg caccagcgcg tatttttcca cgagaaagtt
    10621 caccgcatca cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac gttcatcggc
    10681 aaacggtgtg tcattcagtg tgaggccgta gtgctggtcc agcagtcggg acagcagtat
    10741 ctgccagatt tcaacaggag acgggcgaga actggccgcc tgcccgggta atacaggtaa
    10801 tgttttcata ctgaagattt tcctgatatg cagatataaa aatgggaaag tggcgtggtg
    10861 aaaacaccag gccgtagcag aaggctattc tggagagtta atttttcatt tcgggcgtcg
    10921 gataaacagc cagataaacg taaccacaac tgctgagggt atcggcttca caggccagcc
    10981 ccttcgcata cagcgtgacg gtatgctgat tgcggggatt cagttcaccg ctggtgagca
    11041 tgagttccag ttgtttcatc agcagcggaa aggcctggtc caggtggtac gcatctgcat
    11101 cgctggacag gcctctgata ccggcgcggt cggcaaggta atgcaaccgg ttaccctcct
    11161 gcaccagacg tgccccgaaa cagggcgtca cggtgcaggg cagcccccac caggggcggt
    11221 cgtgattgtc gtcgggaagt gttgtcccgg ggagtgtgtc tgacacgata aaatccttac
    11281 agaaaatcgg ttaagaatgg tccggtggtg gcgataattc tgctcatcag aattcccact
    11341 cagttcaggg tgacgctcat cagccggaca tacgggccaa aactgtcctt acggcgttca
    11401 gcaaacacgg ccagcacacc gggaatatcc tgcacttcac gaccggtata cgcttcagca
    11461 ctgccgtgcc agcggtattt accggtacag aacggaaaaa gacgggatgt cggatgttgt
    11521 tggtgaatac gcatggcttc accacggggg ataattttca taatgggata cctctgaaga
    11581 cagaagataa aagtgaaaac aggtgtgatg tggttgtgac gggttaaagc agaccatgtt
    11641 cggcaaagga gaaaacctgg ttgccaccga ctatcagatg gtccggcacc cggatatcca
    11701 ccaggcccag tgcctgtacc agacgttccg tgataaggcg gtctgccttg ctgggtgtga
    11761 cttcaccgga cgggtgattg tgcgccagca ccacggcagc ggcattgtgg tacagggcgc
    11821 gtttaatcac ttcccgggga tggacttccg tgcggttgat ggtgccggtg aagagggttt
    11881 caccggcaat cagctgattc tggttgttca gatacagcac ccggaactct tcacgctcca
    11941 gtcccgccat gttcagaatc agccattccc gtgccgcacg ggtggaggtg aaggccacgc
    12001 cgggttcatg aagatggcgg tccagggttt tcagggcccg cagaatgagg ctgcgctctc
    12061 tggttgtcat ttctccgggc agaaaggaaa gctgctgcat tgtccctgcc tccattcagt
    12121 cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt
    12181 aataatgaac tgtcatggca taattctctg tacgacaggc gtgatggctg tacgtcatca
    12241 ggcaggtagc aatgccggcg gcttccgggc tcatttcagc gcggttaccg ttcatggcat
    12301 tgaacagtac ccatgtttcg tcatcatctt catcctgttc aggtgccata aatgcgccac
    12361 cgttgttcag ggtgtacaga ttccagatac caccgcagta gttttcgcac agacggtcca
    12421 tccagccgaa gacacggggc tccagggtca cccactgtgg aatgaggcca aaatgctgcg
    12481 gccagaagct gatgcgctgt tcatcaggga tgagggtggc aacaagtcgg gactggtcat
    12541 tccctggtgc tgtggctgaa gaaagagcag ttgtgttctg agaagcgatt ttcatgagtg
    12601 aaatccttac aatgtgtata aatgagagga aataaaggcc gggggctgac aacatcacgt
    12661 tcatcactga ccgtgagacc cggcaatatc agggatgaat gatattcggc tgaaattccg
    12721 gacagccgta cgcgtaaccg ttccggtcac agacggatgg ccagatgcca ttgcctgtac
    12781 tcagtctgca cagactgagc tctccggggc gtcgggtgat tctggtgcct gaatcacgcc
    12841 ttcagggaca gattgcatca gcgcaggctc tccagcatcg tttctgccat cacccacaat
    12901 gcacggttga gcttaatgtc ggtatcaatg ctgtgaatgg cccgggtatg gatacgtttt
    12961 ccttttgcac tgcgaccgga aatcccgcct ttcagcatat tctcctggat ggtctgataa
    13021 gcactccaca ggtccttacc gtaatcctcc cggcgtcgtg gcgtcagaat gtcggcggtg
    13081 gtgacgggct gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg
    13141 cgtgccggtg gcggcaggac cagcgactgc atggcatcac gcttctcctc tatccggtca
    13201 aacacgccca ccacctcgta agccccttca ataactttct ccactacgtc tccccggtgt
    13261 ggaacacgca cttcccccag agactgacca cagacgcagc cgttctggca gacgaacctg
    13321 aagtaacccg gcagcatctg gtagctggag gtaccgtcat gagagttgag aagaataatt
    13381 tcagggacat gctctccgtt tatctctccg gcccgccgca gacgcagcat gtgtttggtg
    13441 tatccccggc ggcccgggtc gcgcacacgg gtctggcagg cgaagaatgg ctgaaagcct
    13501 tcccgctgca ggctttccag tacggtgatg gtggggatgt acgtatagtt ctggcttcgg
    13561 gacgtgtgtt tctcttcccc gaaaatcccc ggcacatggt gcatcagctc ttcatgtgtc
    13621 agtggacggt cacgacgtat ctggtttgca gcataaccaa aacgacttgc taatcgcata
    13681 ctttgctcct tatcggtggt taagatttac tggtgtaata aatgaaaaaa gccacgtctc
    13741 ccggagaaga cgcggcctga cgggtgaaat gaatgaggtt tattgtctga gaagcctttc
    13801 actggcgagc cacgcattaa gcggctttcc ggcaacaatg gctggggctg tatttacctt
    13861 tgatattaat aatgtgcaga gaaacgcatg gtttactcct caggttttaa gtttgtccag
    13921 agccacaacg acaggttttc cggatgtgtc gatgactcct accgtcggta cgccattttt
    13981 gatttcgaag aacggtttgt tgctacctga gcacagctgg ttgtctttgt attcgagcat
    14041 ctgtgctttc ccggttttca ggttaagtac cgagacagtg tagtcgtaac accaggcacc
    14101 accacgttca agcacgaaaa catagctgtt atctttatag ttgaacggat tcttcgtctg
    14161 gcaactcata ccgtcaatct cagtacagga aaacgcctct gtagtattaa cgtatttgat
    14221 gttgtttggc agttttactt gtttcgccgt agcgaagaca gagaacagaa tagttacagc
    14281 aacgagtaaa gttttgataa aagttttcat gacagactcc ttaaattagt taaccgcagc
    14341 tacgtagttt ttcaccagat acgcgaattc atgcacgcca atttcgccga accaaaccag
    14401 gtaggccggg tcatctaaac caaagtagcg gtagccaacg acttcatggg tttttgggtt
    14461 atagagtgtt acgataaagt cccagccgca ggtattggct ttgcaaccgt cgccctggag
    14521 ccagactgta ccgttaatgg ttactggcgt tacgtttccg ccagggccat ccatataccc
    14581 gcctttaccg aacatgccgc tcagtccgac cggttttaat gctttgtcaa gcgcagcttt
    14641 gtaggcagcg tcaccttcgt acaactcgta cgttgttgga cacgctccag gttgttcagc
    14701 ccgggcgttt gggtcgcagc tgagcgcttg tttcacgctc gctgcttcca cgttaaagcc
    14761 agtaaacaga accgccagaa ataacatcag ggtatgtaaa cgtttcattg taaatattct
    14821 tccgttgtaa atggcgctgg aaaatttctc atcagtgaaa ccttccggaa attccccccc
    14881 cttgtatgat gtatatcgcc agtattttat ttcatcccgg atgtaatttt tatatttccc
    14941 ggcacgtgca ataacgctga ccagagatat aaggctcatt aataacttta caggtaattg
    15001 cataattaaa tttccagtga gtgaattgac aggtgagggg taaaactttc tctcaggtaa
    15061 tatatatctg aaaatatttt caataacacc agccaggaaa aacaccacca caggcggtgg
    15121 cgtttctttg gggcgtcttt atttaatccg ggccatcgcg tttctcagtg ccaaatctgc
    15181 ctcttccgtt atctccagac ttttctgcaa caccgtttct gaccgggaca gtacctgacg
    15241 tgctgccagc ggtttccgga tatgctccat cgtcagcatc gtgtcttcat agccctgctg
    15301 acgccaggcg tcggtatgta ccaacgtgaa actgagcaga ccgccgctgt tgtcaccatc
    15361 acctgcatat ttcagccttt tccggggacg gatctcaagg attgtggtgt ccggaaaagc
    15421 ctggcgatcc cataaagaac cgtcactcag atgcgtcaca atcaccatat tgcatccggc
    15481 atcgaccaga ggcgtcacag gggtatttcc ctgcatattt cgccatcctc ccataccacc
    15541 atcaccgaac attgtcccct gaacctcacg gggacggaac agcaggggca gcgcggctga
    15601 cgcaagcaga gcctctttct gctgtccgcg gggcaggctc tggatatgct gaaaaacggc
    15661 gtttttcgtg gttccggcac ccagttcagc acgaatgcag tcaataatat cctgcatgcc
    15721 cccttctgtg gggtacagcg acacatacag cggtagcccg tctgccagag catcagtatc
    15781 aagataatga tccaacagcg ctgtcagggg ctcatctgac aacaacggct gagccatcag
    15841 attgtcaaac ccgttgagta tcgatatatg gcgaagaagc gtcgtaagca gtgctcctgc
    15901 acgtccgggg atctggcaga ggttcattgc ctggaacaat tttttcagca atgaaaagta
    15961 aaccaatcgg tttaccgaca gcacctgatt attccccaga tgatcccaga gcgcctccag
    16021 gcgtacggca gcttctgaca gatcgggaga ggccgcgata atggcaccat tgaatgcgcc
    16081 aatgctggcc cctgaaacca tgctgatctg tgtaccacac tctgccagtg ccttaaccac
    16141 tcccgcctga taagcgccca ccgccccgcc acctgacagc accagccccg ttttcatttc
    16201 tgtactcatg cagccccctt ctgaaatatt tcatcgacca tactttcaga gaggcgttct
    16261 gcagagcaga ggcgaggcat gcgaaccggt aagtgttctg cagcctgcgt ggttgaccag
    16321 atcaccggat gctgctcttc actgcaccag tctcccagcc agttaatcgc gtcatgccac
    16381 tcgttatctg cctgctcccg gccctgacca ccaagccagt ccagagtgtt cagacgcacg
    16441 gttgcactac cgccactctc ctgtgccatc tgcgttaatg ctttatgtct gagaatatcc
    16501 agtagctggt taacctggcc tgtttcgccc tgacgtatca catcaccaaa cgcgccccag
    16561 cgaagctctt caagcgccac ataacaacgt ccggcagggg aaaatgacgc atatcgcccg
    16621 gcgctccacc atgagaatat ctgctcaaga tgtaactgcg cccgctgaac ctggtcaaca
    16681 cggtggagtt tttcttcaag atgattcagt ttatggtgaa cctgatcggc caggatgagt
    16741 aactgctccc gtgtatctgc ggaataatgc gccagcctgg cagtatcact gaccagtgaa
    16801 ctgaccctgt cgtttacccg ggtaatcgcg tagtttgtgg tggcaagtga cgttgtcagc
    16861 tccgtcagcc agcgcaggga cgcctcaagg ccctgtgcaa ccgaagcatt gatggcattc
    16921 tgccgtgcag cgccttcgcc ggtcagtccc tcttttaaac gccggaagaa cgtgcgctct
    16981 ccggccgtgc ggatatgctc ctgtgcaaca tcgattccgt ttgcgaaatc aaccacaaat
    17041 ttttcaggaa gacaggtgag tacctgacat acccggttat cagcaatttg ttgaataaat
    17101 ggtgaagtca cagcattgtc tccagctctt ccctcagcgc cttactgtca gtaatgagct
    17161 caggaaccct ttcatttaat gcctgaagtc gcctggtaag ccgttcctgt gcctgctgat
    17221 cgcgtttatg atcttcagag ctctgaatca gcgtgttacg cagttgttca actttctccc
    17281 tgaatgcgca gaaaaaggca tcgatttcct ggcggacggg ctggttaata tcctgctcaa
    17341 tggatgcctg cagctccccg aaatatgccc gggtcagtga cataacttcc ttcctgacgg
    17401 tgttgatatt gataacactg cggctcacat cctctttata ggtttcccag ccccagtcac
    17461 tggtgccaaa cgctccacaa atctttcccc ataaaccact ctgccgacgg cgacgggtga
    17521 cggtctccgt tctttcctcc ataagcgcac tcagatttgt ttttaccgcg agccgggttt
    17581 gtaactgact gactgccgga aaactgattt ttacggtaaa gccctcatcc tccaggcggg
    17641 tattgatgcg accggcaatg ttttctgtgg catacagggc cgtgccacga aaacctgttt
    17701 caatcccgct gacgattttc tccagctcag gccggaactg tgcttcagcc tcacggtgca
    17761 ggctggtcac ggtcgattcg atttgcgtca tcagttcaag ggcttccctg cgatcactga
    17821 attttatttc ggggctgtcg ggatcaaagt cccgcatcct gtcatgctga tttccggttc
    17881 cgaaaatatc gtgaaagaaa gcgaatgcgt ttctctcccg gggctgcgct gaattttctt
    17941 cctccagcat ttgctgttct ttgcgtttac cgcttctgaa atagtcattc agctcctctt
    18001 ccagtgaggt gcagaccccc gtcagtaatt cccgggttct ggcatcgatc tcctccatgg
    18061 ccattctgac atcagcggtc acctgctcct gacagtctgc tatttcccgg atatcctcaa
    18121 gcaggctggt gatatgcgcc tgcagtgact gaatactttg catcagcccc tgatggcgta
    18181 acgacaggta ttcactgata ttttctgcat tctgcatcag ttttgcggcg gcagaatcaa
    18241 ccgccagtgc cgcagctctg gagtgtgaac tcagtatgac ttccgttatc agttgatcga
    18301 taagtgagca ttcccacagg tctgtcgccc cttcagttgc cagttcgctg tctttacaga
    18361 cgtaatcttt tttcatgcga ccgaaggctt ccctgacaaa atcatcgacc caggcttcat
    18421 caacaggaag ggttccgttt ttccgtaact catgaagcgc acggttagcc aggtatgcct
    18481 ggcgtgagga tccgggataa acgcgggagg cgggcagcac atcgctgttc agcattgccg
    18541 gaactttctg gcgtacggca tctgccccat cgccattgcg gtctttctca tcaaatttat
    18601 tgaccagtac aaacaggcgg ccggctgata cgtcagcaat ggcattaagc tctttacgga
    18661 cgtcttcatc cgcttttgag ttcatctggg tgtaatccat cacagccaga accgcagagg
    18721 ctttctgcag ttgatcccgc atcatcactt ccatctgcgg ttgtccggcc tcattagggc
    18781 cgggggtatc cagcagtgtg agtgttccct ggcatgcatc catccccacc agatggctga
    18841 attccacgtc gatcaccggc agtttctgca cttctgcgta ttcatcaaaa ggaaatccag
    18901 tccccattgc cgcagcaaga cgaaccagat cgtttaacga tgccagtccg gtaaagattt
    18961 cctcctctcc gtggtattca tttttgagcc agccatcatc cgtcagaata tccagcagtt
    19021 cgcgggtatc cccggtttgc tgcagggtct gtgcgacctg ctgtcctgcc ggggtggcga
    19081 gtttttcctg cagtgtgatt aataaattgc ggacaggctg aatatgttcc agatggagaa
    19141 ccggctcagt ttttccggga acgtggcgga tgagcgtcgg tacagaggtc atggggcggt
    19201 tacggttagg cagaatttcc tgccccacaa tggcgttgat ggtggttgat ttgcctgctt
    19261 tcatcgtgcc gacaacagcc agcaccatct ccctgcgggc ggttttgatt tgttcgccct
    19321 ccagctcctc aattcttttc agtgctttct gtttatcaaa aagcagtctg ttttcgttct
    19381 tactgtcaga taacacatct ggctcctcaa ccatttgccg gagcagatta atgttcagtt
    19441 gcaaaagtcg gtcggcttca tcacaaagca gggcgatgtt cttttcgtgc attatttaac
    19501 cttcctgaga cgcattttca tgacaaccac ggaggaggtc atttactaca aatgatcgtt
    19561 tataacgatc gatatgatag taacgatcga tgtattctat taacttggga agcacaacgc
    19621 aaggggacac cccgggtatg gctgcgtttg catagtgttt tcatggagtt aaaaatctgt
    19681 aacaacagcc tggtacatca ccaccgggtt cagaagaaaa tccagttcca taccgcgcgg
    19741 gcaacagaga ccaccgtgtc acgaactgcg cgcagggccg tacggacaac agaggcaaca
    19801 cagacactct ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggtc
    19861 tgagtgcgga cagactccgt gcgcagttcg tcctgcaggc gtgtcatcag agggatggcg
    19921 gcatgtccgg gaagcgctgt catgagtgca ctgaccgggg tgacctcccc ctgaaacagt
    19981 gcattacaga gtgaggattt accggccccg cttttaccca taatgctagg cacgggttcg
    20041 tggctggtga gtttgcgcag atgttccagg atgtgacggg aaagtgagta aggcagggag
    20101 gagagcggtt tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccggtg
    20161 aaaaaagaca aaaccccgcg caccggaaag gtggcggggg gattcacgga gataatatct
    20221 ttctgaaatt attttgaatg caggtatatg tggctgattt gtatcagcta atgagtacag
    20281 gtctgaatct aatctgacag tccgctttgt gccaggagcg gactgtccaa cccatccctg
    20341 aagagcctag attatgtatg gtaattcagt catcaaaata acgttggtca ccatcacaaa
    20401 attcacatcc tcggatatgg ctcagttcag gaacgctatc gctcaggtga ccgcaacaac
    20461 cacatacatg aagctcggtg taatagatga aacactgagt gcaaagatag ccgtctccaa
    20521 atttacatac gctttcagga gtcatgcaat tggtacatcc agcaggaaat acggagagta
    20581 gctgttcatc cacactgtgg aacaactcct catccagcag tttataccta tccgaagtgt
    20641 agtcgcagtg ttcgcattca aaatcactat ctccctcagg tagcaattga ggtatttcgc
    20701 agtctggaca aactagggta atttgcctta cagcactggt gcaaacctta caccgcgtaa
    20761 cttcaagctt acggttttca ttaccggtcg tttcagtccc tgttaccgtc gcatgctgat
    20821 ggcactcatt gcagttaccg atttgaatat tcttttcaga gagctgctcg agttcaggct
    20881 taacctgttt aaaccgcacc tcggcataaa atttgtttcc cctaatcaga cgagtttcac
    20941 cgaaagccag cttccaatta tgtttgacac cgaagataac tttccattca tcccttaata
    21001 accggttcag ggcaaaccag gcgtcagctt gttctttgag tatttgacgt tgttcagcct
    21061 cggtgaaagt ggggtggtag aaatgaacta cgcggttgcg gtgctttctg accttatcaa
    21121 aagcactgtc cgtttctctg tctaggggtc tttcaagtac gtccttaagc ctttcacggg
    21181 tttcttcgta ggtcaccgac tgaaaatcac cggctaggta agcctgcttt tttattggct
    21241 ttttcgggct gcagaccaac gaccagtgtt cgtgcgccag cggaactttc agtaaaatct
    21301 cgaccgcagt ccagaaactg acgacggaga acttcggctt cgaggcctcc aactcttcac
    21361 gggctttatc cagaaaatcc agaccattgt tgatcagcgc ctgcacatcc tgactgagtt
    21421 cacggggggc gggagtatcg ggtgcagtat cgtcaacatc gaacatcatt atctccttta
    21481 atcagcttcc accacgcagt tgctacagcg cgttagttgt atccagtgta aactggactt
    21541 ggtagctggc agccacatcg agcgcaaaaa accgtaaatt aacttctgta gactcgtact
    21601 tttcgtgacc catgcgtacc cgcaggatct tcgggtgcac gtgaccataa agcaggtgcc
    21661 taccaaagct gtggcgattc agatccctaa actgatgggg tcccaaaaag tatgccatag
    21721 caagtgtaag tatccctaaa atctgcacta ttctcttttt ataaatcctc tgacatgtga
    21781 attagattgc cctaaagtga gcttccgcgc ctcgctcata aggacaacca tactcaaatc
    21841 tcccacattg caggagattt gagtatgaac acgtcaccgt ggaacaaaga ccgtatcata
    21901 ggccaaaaaa gaccacttca gatatctcat atctggggta tccgaatccg acttgaactg
    21961 gaaggtaaaa cacgcgattt agctctgttc aacatggcct tggacagtaa gcttcgaggc
    22021 tgtgatctag tcaaactcaa agtatctgat gttgcatatg gtagctctgt ttcaagcaga
    22081 gctacggttc tacaacagaa aaccggcagc cctgtccaat ttgagataac caaagggaca
    22141 agagaagctg tttctgcatt gataaagctt ggcaatttac gcagtaaaga ctatttgttc
    22201 cgatctcggg tcggtactaa ccaacacata tcaacccggc aatacaaccg aatttttcat
    22261 gggtgggtag caaaacttgg tctcgaagat tcgctttaca gtacacattc cttgagaaga
    22321 acaaaacctt acctgattta caagaaaact aagaatctca gggtgatcca acttctgtta
    22381 ggccataaga aactggaaag cacagtccgt tatctgggca ttgaagtcga tgatgcgtta
    22441 gagatctctg aatcgattga agtctaaggt tgccagggct gcaacagcag tcctgtgcca
    22501 gatgcggacg ttgctctatt catgtaagat taatagaatg tctttcgata aatttttgac
    22561 ttaatcttaa ataagaatga cttttttgca tg
//
LOCUS       DAJOTB010000075        22380 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJOTB010000075 DAJOTB010000000
VERSION     DAJOTB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.075"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            116..331
                     /gene="bdm"
                     /locus_tag="OHN28_002780"
     CDS             116..331
                     /gene="bdm"
                     /locus_tag="OHN28_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCQ2298151.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            433..570
                     /gene="sra"
                     /locus_tag="OHN28_002781"
     CDS             433..570
                     /gene="sra"
                     /locus_tag="OHN28_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCQ2298152.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            726..2423
                     /gene="maeA"
                     /locus_tag="OHN28_002782"
     CDS             726..2423
                     /gene="maeA"
                     /locus_tag="OHN28_002782"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2298153.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2557..3567
                     /gene="adhP"
                     /locus_tag="OHN28_002783"
     CDS             2557..3567
                     /gene="adhP"
                     /locus_tag="OHN28_002783"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCQ2298154.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3753..4030
                     /locus_tag="OHN28_002784"
                     /pseudo
     CDS             3753..4030
                     /locus_tag="OHN28_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4030..4314
                     /locus_tag="OHN28_002785"
     CDS             4030..4314
                     /locus_tag="OHN28_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2298155.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4364..5017)
                     /gene="fdnI"
                     /locus_tag="OHN28_002786"
     CDS             complement(4364..5017)
                     /gene="fdnI"
                     /locus_tag="OHN28_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCQ2298156.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5010..5894)
                     /gene="fdnH"
                     /locus_tag="OHN28_002787"
     CDS             complement(5010..5894)
                     /gene="fdnH"
                     /locus_tag="OHN28_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCQ2298157.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            complement(5907..8954)
                     /gene="fdnG"
                     /locus_tag="OHN28_002788"
     CDS             complement(5907..8954)
                     /gene="fdnG"
                     /locus_tag="OHN28_002788"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8367..8369),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2298158.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9186..10067
                     /gene="yddG"
                     /locus_tag="OHN28_002789"
     CDS             9186..10067
                     /gene="yddG"
                     /locus_tag="OHN28_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCQ2298159.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10340..11728
                     /gene="narU"
                     /locus_tag="OHN28_002790"
     CDS             10340..11728
                     /gene="narU"
                     /locus_tag="OHN28_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCQ2298160.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            11823..15563
                     /gene="narZ"
                     /locus_tag="OHN28_002791"
     CDS             11823..15563
                     /gene="narZ"
                     /locus_tag="OHN28_002791"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCQ2298161.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            15560..17104
                     /gene="narH"
                     /locus_tag="OHN28_002792"
     CDS             15560..17104
                     /gene="narH"
                     /locus_tag="OHN28_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2298162.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            17104..17799
                     /gene="narW"
                     /locus_tag="OHN28_002793"
     CDS             17104..17799
                     /gene="narW"
                     /locus_tag="OHN28_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2298163.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            17796..18476
                     /gene="narI"
                     /locus_tag="OHN28_002794"
     CDS             17796..18476
                     /gene="narI"
                     /locus_tag="OHN28_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2298164.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            18556..19449
                     /locus_tag="OHN28_002795"
     CDS             18556..19449
                     /locus_tag="OHN28_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCQ2298165.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLSHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(19545..20390)
                     /gene="nhoA"
                     /locus_tag="OHN28_002796"
     CDS             complement(19545..20390)
                     /gene="nhoA"
                     /locus_tag="OHN28_002796"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCQ2298166.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            20565..21134
                     /locus_tag="OHN28_002797"
     CDS             20565..21134
                     /locus_tag="OHN28_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCQ2298167.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            complement(21205..22341)
                     /locus_tag="OHN28_002798"
     CDS             complement(21205..22341)
                     /locus_tag="OHN28_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2298168.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITPIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         5322 a         5832 c         5701 g         5525 t
ORIGIN      
        1 catcaaccag gactaatctt aacaacgaag cagcaaatat ttgccatgct gaatgtgctt
       61 tataaatctg cgatccgtag cagacaccat aaatacacag acacggagaa tcactatgtt
      121 tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg ccattgagca
      181 aggccttcgg gcagagcacg gtgttgtcac tgaagatgac attctcatgg agctgaccaa
      241 atgggtggaa gcttctgata acgacatcct cagtgatatc taccagcaaa cgatcaatta
      301 tgtggtcagt ggtcagcacc ctacgcttta aggtgctatg cttgatcggc aacctaattt
      361 aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat
      421 tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact ggaccataaa
      481 atttctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt aaataatccc
      541 accggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca atgacgatta
      601 aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt ggtgccgacg
      661 ccctggcggt aatgcaaaga cgataaaagc ccccagggat ggatattcaa aaaagagtga
      721 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      781 tattgctgga atttccgttg ttgaataaag gcagcgcctt cagcatggaa gaacgccgta
      841 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      901 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      961 acatccagga cactaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
     1021 tgatgccagt catttacacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
     1081 accgccgttc acgcggtgtg tttatctctt accagaaccg ccacaatatg gacgatattc
     1141 tgcaaaacgt accaaaccat aatatcaaag tgattgtggt gactgacggc gaacgtattc
     1201 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
     1261 ataccgcctg tggcggcatc agtccggcct atacccttcc ggtggtgctg gatgtcggca
     1321 ccaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
     1381 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
     1441 cggacgtact gttgcagttt gaagacttcg cacaaaaaaa tgcgatgccg ttacttaacc
     1501 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcaccgcg gcggtaacag
     1561 tcggcacact gatcgcagcc agccgcgcgg caggcggtca gttaagcgag aaaaaaatcg
     1621 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1681 agcgcgaagg attaagcgag gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg
     1741 gcctgctgac cgacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1801 gcgaaaatct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1861 gtaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1921 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1981 cgacttcacg cgtggaagcc acaccgcagg acattatcgc ctggactgaa ggtaacgcgc
     2041 tggtcgccac tggcagtccg tttaatccag tggtatggaa agataaaatc taccctatcg
     2101 cccagtgtaa caacgccttt attttcccgg gcatcgggct gggtgttatt gcttccggcg
     2161 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacacttgct cagtattcgc
     2221 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     2281 cccgcgcaat tgcctttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa
     2341 cgtctgccga agctctgcaa caggccattg atgataattt ctggcacgcc gaataccgcg
     2401 actaccgccg tacctccatc taagcctgcg cccggtagtg agggctaccg ggcaattttc
     2461 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     2521 cctgctatgc agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag
     2581 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2641 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2701 ggtgacaaaa ccggcgtgat tctgggacac gaaggtattg gtgtggtggc agaagttggc
     2761 cctggtgtta cttcattaaa accaggcgat cgtgccagcg tggcgtggtt ctatgaagga
     2821 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2881 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg
     2941 gtaaaagtgc cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc
     3001 actacctaca aagccgttaa gctgtcaaaa attcgtccag gacagtggat tgctatctac
     3061 ggtcttggcg gtctgggtaa cctcgccctg caatacgcga agaatgtctt taacgccaaa
     3121 gtgatcgcca ttgatgtcaa tgatgaacag ttaaaactgg caaccaaaat gggtgcagat
     3181 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa agccggtggc
     3241 gctcacgcag cggtggtgac agcggtagct aaagctgcgt ttaactcggc ggtggatgct
     3301 gtccgtgcag gtggtcgtgt cgtggctgtc ggtctgccgc cggagtctat gagcctggac
     3361 atcccacggc tggtgctgga tggtattgaa gtggtcggtt cgctggtcgg cacgcgtcag
     3421 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     3481 cgtccgttag aggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     3541 cgtatggtga ttgatttccg tcgctaagag gtctttgctg cgactgccat gttcgggtcg
     3601 cagcctgttt cgcgagacgc tttccgtaat ttcactcttc ttcttttcct cccccatatt
     3661 tcccctcaca attcgcgaaa acgtagcgaa atttgttgcg ctgcacggaa caatagtgat
     3721 agtgttcctt ttgagggaac aaggatgaag agatgataat gaatttcaga cacaagggat
     3781 tgcgtgactt gtttcttctc ggcaaaacct ccggcgttat accgacgcaa gtcaaacgat
     3841 tacgccacca ctcgctgtga ttgatgcagc atgctgtctt gctgatatcg atatgcccgg
     3901 ttaccgacta catccgttaa gcggcgatcg cgatggaatt tgggcgatat ctgtctcggg
     3961 caactggcga atcacatttg aattcgtcaa tggcgatgca tacatactgg attacgagga
     4021 ctatcactga tgaaaatggc caatcatccc cgcccggggg acattattca ggaatcactg
     4081 gacgaactta atgtcagcct gcgcgagttt gccagagcaa tggaaattgc gccctcaacg
     4141 gcaagccgat tgctgaccgg aaaagcagct ttgacgccag aaatggcaat aaaactttcc
     4201 gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc aaaatgcctg gagtctggca
     4261 gaggcggaaa aaacggtgga tgtgtcgcga ctgcgccgtc tggttacgca ataaaaaatg
     4321 gcgcttctta aagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac
     4381 tctctttttt cgcttctgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg
     4441 cccagcgacg gctcaccttc ccttcgatca tccctttaat cgatcctttc acccagaatg
     4501 ccatatacat atggatcagg atggcgtgga tcaggatgat acccgcagcc gcatggatca
     4561 gcaggctata acggacaacc tgtatcggga agtactgcgc aaagtacgga cgccagataa
     4621 tcactccggt caccagcagc acgaaaatca tgctcatgat tgaccagaac atcatctttt
     4681 gcccggcgtt gtacttaccg acatccgcca ctttgtgctc attgcctttt aatacttcga
     4741 caatgttcaa cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca
     4801 caaacataaa catcagcgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc
     4861 ccatctgcgg cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa
     4921 tcccggacag cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa
     4981 taaatttggt gcgcacaatc attttcgact tactcatgat ttccctcctc ctcatcgtcc
     5041 acttccttat tcgggccaat accgatgtag tggaaaatca acccggcaaa agtggcaata
     5101 aagccagccg ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtatcgatc
     5161 ttcggatctt tcggcagacc atgatacagc tccggctgat cggcgtgatg cagcacgtac
     5221 ataacgtgcg taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc
     5281 gctttcagtt tcgccacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag
     5341 tggatagccc cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga
     5401 tcgacacaga gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta
     5461 aacggacacc cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcgaca
     5521 atcccgttag cgtactgaat gattgcacca gcagacgggc atgccttcag gcagcccgga
     5581 tcttcacagt gcatacagcc gtctttacgg atcagccact ccagcttgcc gttctgttcg
     5641 gtttcgctaa agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa
     5701 accccgacgc agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga
     5761 caggctttac agccgataca agtggaaacg tcgataagtt tcgcgacttc tgctttgtaa
     5821 tcacgcacct gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc
     5881 gtttccatag ccatctgttc gcccccttac gccttctcga tgtttactaa gaacgcttta
     5941 tattccggcg tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa
     6001 cctttacgcg cgacaccttc aaagccccag tggattggaa tacccaccgt ttcaacctgc
     6061 tgaccgttta cattcagcgg tttcagacgg cgcgttacca cagccaccgc gcggataaag
     6121 ccacgcttgc tggagacagt gacacgatcg ccattggcaa tgcctttcgc cgccgccagc
     6181 gtttcgctga tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg
     6241 gtccaggtgt ggaagtgctc cgtcagacga taggtcgtac ccacatacgg gaactgctct
     6301 tttttaccca tccgcagcgc gtcttgttca tacagacgaa caaccgggtt agacaccacg
     6361 ttcggatgca acgggttagt gcccagcggc gtttcaattg gctcgtagtg ttccgggaac
     6421 ggaccttccg ccattttgtt gatggcaaac aggcgtccca tcccttccgg ttgcatgata
     6481 aacggcccgg ttggcgtacc cggcgcggca ttgccgaagt ccggaatatc gttacccgtc
     6541 cacttgctac cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata
     6601 tccgccgaag cacggttgta gagtacgcga cggttgagcg gccacgccca ggcccatccc
     6661 agcgtattcc ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc
     6721 tcggtccagc taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga
     6781 tgtgcaaagc tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcgtcatag
     6841 agatcttcca gcgcatagcc gttattctct ttcgccactt catcagactg cggttcgtgc
     6901 ggctgcttgt agttccagct catcttcatc agcggttcta cgcctttacc accttcggcc
     6961 tggtacagct cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgtgct
     7021 tcgcccggcg cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata
     7081 gaaccatctt cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagat
     7141 gccggatcga catcgttcga ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc
     7201 agcggatcga taaccaccat gtacttcagc ttgctcaggc agctcaccac tttgtttttg
     7261 tccgggaagg acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttaccttca
     7321 tccatcatgt tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtcg
     7381 tagccccagt tgttctcttt ctgcgcggca tcgccgtaga aagatttcat caggctaacg
     7441 aaaaacttcg gatagttgct ccagtagttc acctgatcag ccagcgtcgc tttcggcgtg
     7501 ttcgcttcca gatacgactg caaatcaacc tgtttttctg acggcagcgt cagataacct
     7561 ggcaggctgg tagagagcag gcctaagtca gtcaggccct gaatgttgga gtgaccacgc
     7621 aatgcgttca cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc
     7681 atcgtacgga tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag
     7741 gtggttgtgc gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg
     7801 gcttttggcg taccgcaaat gttttctact acgtccggcg tgtagcggga aacgtgcgct
     7861 ttcagcagat tccatacaca gcgcggatga gtcagcgtgt catcgcgttt cgcatagccg
     7921 ttttcatcga actgatagtt ccaggacgac ttatcgtatt gacgtttttc agcgtcgtag
     7981 ccgctgaaca gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg
     8041 taatgcttaa cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca
     8101 gacaggaacg taatgtccgt accggaacga ataggtgcgt aaatatccgc cacagaagcg
     8161 gtacgcgtaa aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc
     8221 atcgcccagc ggaaaccgac gggatgcgct tcagcagcgt taccgcccat caccattacg
     8281 acgttagcgt ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     8341 gcaagacttg ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc
     8401 ccgagggagc gggcaaattt ttgagtcagc atcccggttt cgttgctggc accggaagca
     8461 cacagcatac cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata
     8521 aagttagcgt cacggtcggc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag
     8581 ctaatgcgct gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt
     8641 tcgctgttga cataatccag caaaccggcc cctttcgggc acagcgcacc acgacttacc
     8701 ggatgatccg ggtcaccttc aatgtgataa atcgcttctc tggcgttttt cgcgccatca
     8761 cccaggctat acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc
     8821 tctttcgcgc gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag
     8881 cccaatgctg ctactgttgt tcccgccata ccgcccgcgc agattttaaa aaattgtctg
     8941 cgactgacgt ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca
     9001 gtaaaattct gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat
     9061 ccgcctgcgc aaattgacct acctcaatag cggtagaaaa acgcaccact gcctgacagg
     9121 ccagttaaaa aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag
     9181 cgagcatgac acgtcaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca
     9241 cgatggtagg attgattcgc ggtgtcagtg agggactcgg cccggtcggc ggcgcagctg
     9301 ctatctattc attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc
     9361 agatcccgaa aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc
     9421 tggcgctttc cttagggtat gcggcgaccc gtcatcaggc gattgaagtg gggatggtga
     9481 actatctatg gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca
     9541 actggctgat tgtaccagga ttattattag ccctggtcgg cgtctgctgg gtgttaggcg
     9601 gtgacaatgg gttacattat gatgaaatta tcaataatat caccaccagc ccattgagtt
     9661 atttcctggc gttcattggg gcgtttatct gggcagtcta ctgcacagta acgaataaat
     9721 acgcacgcgg atttaacgga attaccgttt ttgtcctgct aacgggagca agtctgtggg
     9781 tttactattt tcttacgcca caaccagaaa tggtatttag cacgcccgtc atgattaaac
     9841 tcatctctgc ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatattac
     9901 atggcaacgt caccataatg gcggtaggtt cgtattttac gcccgtcctt tcctcagcgc
     9961 ttgcagccgt gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctaatgg
    10021 tctgcggcgg ttccctgctc tgctggctgg cgacacgtcg tggctaaaat aattgtcggg
    10081 tctacggccc ggcagttaat cagataaatt ctgacaatat taaaaaataa atttcaaata
    10141 ccagaaagtc attttagtta catttagtaa cacatatctg tctgtttaat ccacattctg
    10201 cccccgcagt tacgctttct tatctattct tctaatcagc acttcacctg attcatcctt
    10261 aattctaata actggctatt gcgccttacc tcctgttttg tctttttttc attaccaatg
    10321 tgtgcatgtg aggaacaata tggcactgca aaatgagaaa aatagtcgtt atcttttacg
    10381 cgactggaaa ccagaaaatc cggccttctg ggaaaataaa ggaaagcata ttgcacgaag
    10441 aaacctctgg atatcagtca gttgtctact tcttgccttc tgtgtctgga tgctatttag
    10501 cgcagttact gttaatctca ataaaatcgg ttttaatttt actaccgatc aactcttttt
    10561 attaaccgca ttaccctccg tttctggcgc attattgcgt gtcccctact cctttatggt
    10621 gcctatattc ggtggacgcc gatggacggt ttttagtact gcaatcctga ttattccttg
    10681 cgtctggctc ggaattgccg tgcagaatcc gaatactcct tttgggatat ttatcgttat
    10741 cgctttgcta tgcggttttg caggtgcgaa ctttgcttcg agcatgggca atatcagttt
    10801 cttctttcca aaagccaaac aagggagcgc tcttgggatt aatggcggat taggaaactt
    10861 aggtgtaagt gtaatgcagc tggttgcacc gctggtcatt tttgtccccg tatttgcctt
    10921 tctcggcgtc aatggcgtac cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc
    10981 atggatttgg gtgccattac tggcgattgc cacgatcgcc gcctggtcag ggatgaatga
    11041 tatcgccagt tcacgcgcct caattgccga ccagctccct gtcttacaac gcctgcatct
    11101 ctggctgctg agcctgcttt accttgccac cttcggttcg tttatcggtt tttctgcggg
    11161 ttttgccatg ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc tggcgttctt
    11221 tggcccattt atcggtgcca tcgcgcgatc ggttggtggt gctatttccg ataaattcgg
    11281 cggcgtgcgg gtgacgttga tcaacttcat ttttatggcg attttcagcg ccctgctgtt
    11341 ccttacctta ccgggcacag gctccggtaa tttcatcgca ttttacgccg tatttatggg
    11401 gctgtttctg accgcgggtc tgggaagtgg ttctactttc cagatgatcg ccgtcatctt
    11461 tcgccagata acaatttatc gggtgaagat gaaaggcggt agtgatgagc aagctcaaag
    11521 agaagccgtc accgaaacgg cagccgctct gggctttatc tcagccattg gcgcagtggg
    11581 cggctttttt attccgcagg cgtttggcat gtcgctcaat atgaccggct ctccggtggg
    11641 cgcgatgaaa gtgtttttaa tcttctacat cgtttgtgtg ctactgacct ggctggttta
    11701 tggtcggcgg aagttcagcc aaaaataaga gatcgatgcc tgacacgtct tatcaggcta
    11761 caaccgtgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc aggagttatg
    11821 tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa atctttgccg
    11881 atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat cgccagcgtt
    11941 ggcagttcga caaaatcgtg cgttccaccc acggtgttaa ctgtacaggc tcctgtagct
    12001 ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc gattacccgc
    12061 gcactcgccc tgacctgccg aatcacgaac ctcgcggctg cccgcgtggc gcaagttact
    12121 cctggtatct ctacagcgcg aaccgcctga aatacccgct cattcgtaaa cgactgattg
    12181 aactgtggcg cgaagccctc aagcaacaca gcgatccggt actggcgtgg gcatcgatta
    12241 tgaacgatcc gcaaaagtgc ctgagctaca aacaagtgcg tggacgcggt ggttttatcc
    12301 gctccaactg gcaggagcta aatcagctga ttgccgccgc taacgtctgg accatcaaaa
    12361 cttacggccc ggatcgcgtt gccggtttct cgccgattcc ggcgatgtcg atggtttctt
    12421 acgctgccgg aacacgctat ctgtcgctgt taggtggcac ctgtttaagc ttctacgact
    12481 ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc gacgtaccgg
    12541 aatctgccga ctggtataac tccagttaca tcatcgcctg ggggtctaac gtaccgcaga
    12601 cacgtacgcc ggacgcccat ttctttaccg aagtacgcta caaaggcact aaaaccatcg
    12661 ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg gcaccgaaac
    12721 aaggcactga tagtgccctg gcaatggcaa tgggccatgt cattttaaaa gagtttcatc
    12781 tcgataatcc cagcgattat tttatcaact actgccgccg ctacagcgac atgccgatgc
    12841 tggtaatgct ggaacctcgc gacgacggca gctacgttcc cgggcgcatg gtccgcgcat
    12901 ctgacctggt ggatggactg ggcgaaagca ataatccgca gtggaaaacc gtggcagtta
    12961 ataccgcagg tgaattggta gtgccgaacg gttcgattgg ttttcgttgg ggagaaaaag
    13021 gcaaatggaa cctggaatcc attgccgccg gtacggaaac cgaattgtcg ttaaccctgc
    13081 tcggtcaaca tgacgccgtt gcaggcgtgg ccttccccta ctttggaggc attgaaaatc
    13141 cgcattttcg cagcgtaaaa cacaatcctg tcctggtgcg ccaattgccc gttaaaaatc
    13201 tgacattagc cgatggtagc acctgtccgg tggtcagcgt ttatgatctg gtactggcaa
    13261 attacggcct cgatcgcggg ctggaagatg aaaacagcgc gaaagattac gctgaaatca
    13321 aaccttacac ccctgcctgg ggcgagcaaa ttaccggcgt accgcgccag tatattgaaa
    13381 ccatcgcccg tgaatttgcc gataccgctc ataaaacgca tgggcgctcg atgattattc
    13441 tcggcgcagg tgttaaccac tggtatcaca tggacatgaa ctaccgcggg atgatcaata
    13501 tgctgatctt ctgcggttgt gtcgggcaaa gcggcggcgg ctgggcgcac tatgtcggtc
    13561 aggaaaaact gcgcccacaa accggctggt tgccgctggc ctttgcgttc gactggaacc
    13621 gtccaccgcg ccagatgaac agcacctcgt ttttctacaa tcactccagc cagtggcgct
    13681 atgaaaaagt caccgcgcag gaattgctct caccgctcgc cgatgccagt aaatacagcg
    13741 gtcacctgat tgatttcaac gttcgcgccg aacgtatggg ctggctccct tctgcgccgc
    13801 agctggggcg taacccgctc tccctgaaag ctgaagccga caaggccgga ttatccccca
    13861 cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc gaacaaccag
    13921 atagcggcag caatcatccg cgtaatttgt ttgtctggcg ttctaacctg cttggctcct
    13981 ccggcaaagg ccacgagtat atgcagaagt atctgctggg gaccgaaagc gggattcagg
    14041 gagaggaact cggtgccagc gacgggatca aaccagaaga agtcgagtgg caaactgcgg
    14101 cgattgaagg caagctcgac ctgctggtga cgctcgattt ccgcatgtcc agtacctgcc
    14161 tgttctccga tatcgttctg cccaccgcca cctggtacga aaaagacgat atgaatacct
    14221 cggatatgca tccgtttatt catccgcttt ctgcggcggt cgatccggcg tgggaatcac
    14281 gcagcgactg ggaaatctac aaaggtatcg ctaaagcctt ttcgcaagtg tgcgtggggc
    14341 atctcggcaa agaaaccgac gtggtattac aaccactgct gcatgactcc ccggcagagc
    14401 tttcacagcc gtgtgaagtg ctcgactggc gcaaaggcga gtgcgatctg attccaggca
    14461 aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacctat gaacgcttta
    14521 cctcgctcgg gccattgatg gacaaacttg gcaacggagg taaagggatt tcgtggaata
    14581 cccaggatga aattgatttc ctcggtaaac tcaattacac caagcgtgat ggcccggcac
    14641 aggggcgtcc gctgattgat accgcgattg atgcctcaga agtgattctg gcgctggcac
    14701 cggaaaccaa cggccatgtt gcagtcaaag cgtggcaagc gctgggcgag atcaccggac
    14761 gcgaacatac ccatctggcg ctgcacaaag aggacgagaa gattcgcttt cgcgatattc
    14821 aggcgcagcc gcgtaaaatt atctccagcc ccacatggtc tggtctggaa agcgatcacg
    14881 tctcctataa tgcgggatac accaacgttc atgagctaat tccgtggcgc acactgtcgg
    14941 gccgtcaaca gctttatcag gatcatccgt ggatgcgtgc ctttggtgaa agcctggtgg
    15001 cttatcgccc gcctatcgac acccgcagtg tcagtgaaat gcgccaaatc ccgccaaacg
    15061 gcttcccgga aaaagccctt aacttcctga caccgcacca gaaatggggc attcactcaa
    15121 cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt gtctggatca
    15181 gcgaaaccga tgcccgtgaa ctgaccattg tcgataacga ctgggtggaa gtgtttaacg
    15241 ccaatggcgc gctgacggcc cgcgcggtgg tcagccagcg tgtaccgccg ggcatgacca
    15301 tgatgtatca cgctcaggaa cgcattatga atattcctgg ttcggaagta accggcatgc
    15361 gcggcggcat tcataactcg gtcacccgcg tttgtccgaa accaacacat atgattggcg
    15421 gttacgcgca gctggcctgg ggctttaact actacggcac tgtcggctct aaccgcgacg
    15481 aattcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc
    15541 aggttcagga ggcgaaaaaa tgaaaatacg ttcgcaagtc ggcatggtgc ttaacctcga
    15601 taaatgtatc ggctgccata cctgttcggt gacctgtaaa aacgtctgga ccggtcgcga
    15661 aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa
    15721 aaactgggaa gatcaggatg agtggcaagg tggctgggtg cgtgatgtga atggcaagat
    15781 acgcccgcgt ctgggcagca agatgggcgt aataaccaaa attttcgcca acccggtagt
    15841 gccgcagatt gacgattact acgaaccttt caccttcgac tacgaacatt tgcatagcgc
    15901 gccggaaggc aaacatattc ccaccgcccg tccgcgttca ctgattgacg gcaaacggat
    15961 ggacaaagtg atctggggcc caaactggga agaactgctg ggcggcgaat ttgaaaaacg
    16021 agcccgcgac cgcaacttcg aggccatgca aaaggagatg tacgggcagt ttgaaaacac
    16081 cttcatgatg tacctgccgc gcctgtgcga acactgcctc aatcccagct gcgtggcgac
    16141 ctgccccagc ggcgctatct acaaacgcga agaagacggc attgtgctga ttgatcagga
    16201 taaatgccgt ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa
    16261 ctggaaaagc ggcaagtcag aaaaatgtat cttctgttat ccgcgaattg agtccggtca
    16321 gccaacagtc tgctccgaaa cctgcgtggg tcgcatccgg tatctgggcg tgctgctcta
    16381 cgacgccgac cgcatcgaag aagcagcgag taccgaacga gaggtcgatc tctatgaacg
    16441 ccagtgcgaa gtgttcctcg atccgcacga tccctcagtg atcgaagaag ccctgaaaca
    16501 aggtattcca caaaacgtga ttgacgctgc ccagcgttcg cctgtctaca aaatggcgat
    16561 ggactggaaa ctggcgctac cgctgcaccc tgaataccgc accctgccga tggtctggta
    16621 cgttcctccg ctgtcaccga ttcagtccta cgctgatgcg ggaggtttgc cgaaaagcga
    16681 aggcgtgctg cccgccatcg aaagcctgcg tattccggtg caatatctcg ccaatatgtt
    16741 gagtgccggt gataccggtc cggtactgcg ggcgctgaaa cggatgatgg cgatgcgcca
    16801 ctatatgcgt tcacaaaccg tggaaggcgt tactgatact cgtgccatcg acgaagtagg
    16861 tctgagcgtc gcccaggtcg aagagatgta tcgttacctc gccattgcca actatgaaga
    16921 tcgtttcgtc atcccgacaa gccatcggga aatggcgggc gatgccttcg cagaacgcaa
    16981 cggctgcggt tttacctttg gcgacggttg ccacggttcg gacagcaaat tcaacctgtt
    17041 caacagtagc cgtatcgatg ccatcaacat caccgaagtg cgcgacaaag cggagggcga
    17101 ataatgcaga tcctcaaagt gctcggcctg ttgatggagt acccagacga gttgttgtgg
    17161 gagtgtaagg acgacgcgct ggcgcttatc cgccgcgacg cgccgatgct caccgatttt
    17221 gcactcgacc tgctcaacgc cccgctgctg gataaacagg ccgaatggtg tgaagtgttt
    17281 gaccgcgggc gcaccacgtc gctgctgctg tttgaacatg ttcatgccga gtcccgcgat
    17341 cgcggccagg caatggtgga cctgctggcg gagtatgaaa aggtcggtct gcaactgaat
    17401 tgtagggaac tgcccgatta tctaccgctg tatctggagt atttaagcgt acttccggac
    17461 gatcaggcga aagaaggatt actcaacgtt gcgccgatcc tcaccctgct tggcggacgc
    17521 ttaaaacaac gcgaggcacc gtggtacgcg ttatttgacg ccctgttgca actggcagga
    17581 agccctctgt caagtgacag tgtcacaaaa cagattcgca gtgaagagcg tgacgacacc
    17641 cgccaggcgc tggatgcggt gtgggaagaa gaacaggtga agtttattga agataacgcc
    17701 acggcgtgtg acagctcgcc gcttaatcaa tatcagcgac gctttagcca ggatgtcgcg
    17761 ccgcagtatg tcgacatcag tgcgggaggt gggaaatgat tcagtatctg aacgtctttt
    17821 tttacgatat ctacccgtac ttgtgtggca cggtttttat ccttggaagc tggctgcgct
    17881 acgactacgg gcagtacacc tggcgcgccg cctccagcca gatgctggat aaacgcggga
    17941 tggtgctgtg gtcaaacttg ttccatatcg gcattttggg gattttcttc gggcacctgt
    18001 tcggcatgtt aacgccacac tggatgtacg cgtggtttct gccggtggcg gtaaaacagc
    18061 agatggcgat gatcctcggc ggaatatgcg gggtattgac gctgattggc ggtgcggggc
    18121 tactggtgcg tcgtttaaca aatccgcgta tcagggcgac gtcttctggt gcggatatcc
    18181 tgatcctcgc cattttgtta attcagtgca ttctcgggtt aaccaccatt cctttttcag
    18241 cacaacatcc ggacggcagt gaaatgatga agctggtggg ctgggcgcaa agcattgtca
    18301 ctttccaggg cggtgcatct acgcatcttg atggtgtcgc ctttatattc cgcgttcatc
    18361 tggtgcttgg aatgaccatc ttcctgctct tcccgttcac ccgactggtg catgtctgga
    18421 gcgcgccgtt tgagtacttt actcgtcggt atcagattgt gcgttcgcgg cgttaattct
    18481 cacgcgggcc ggatagccct tcgctctccg gccacttgca ggctatgata gttctccagc
    18541 cacaggagaa cgttaatgaa accgcaagtg taccacgtcg atgcttttac ctctcaaccg
    18601 tttcgcggca attctgccgg tgttgttttc cccgccgata atctcagtga agcgcaaatg
    18661 cagcttatcg cccgcgagtt aggtcattcg gaaactgctt ttctgctgca cagcgacgac
    18721 agcgatgtgc gcatccgcta ctttacgcca acggttgaag tgccaatttg cggtcacgcg
    18781 acggtggctg cgcactatgt acgttcaaag gtattaggtt tgggaaattg cacggtctgg
    18841 caaacgtcgc tggcaggaaa acatcgtgtg actatcgaaa agcaaaatga tgattatcgt
    18901 atttcgctgg aacaaggtac gccaggcttt gagccaccac tgacaggtga aacacgtgca
    18961 gcgattatca acgcgcttca tctcactgag gacgatattc tgccaggctt gccaattcag
    19021 gtggcaacaa caggacactc aaaagtgatg atcccgttga aaccagaagt ggatatcgac
    19081 gccctttcgc ctgatcttgc tgcgttgacc gctatcagca aacagattgg ctgcaatggt
    19141 ttcttcccgt tccagatccg cccaggcaaa agcgaaaccg acggtcgcat gttctcgcct
    19201 gcgattggta ttgtggaaga tccggtgacc gggaatgcca atggtccgat gggcgcatgg
    19261 ttagtacatc acaacgtatt gtcccacgat ggcaacgtgt tgcgtgttaa aggccatcag
    19321 gggcgcgcat tggggcgtga tggtgtaatt gatgtgacgg tgacaattcg cgataaccaa
    19381 ccggagaaag ttaccatttc cggcacggcg gtgattctat tccatgctga atgggccatt
    19441 gatttttgat gccgttgcct gatgcgcttc gcttatcagg cctacaaccg gactaatgta
    19501 ggccggataa ggcatttacg ccacatccgg caacctgaca taaattattt tcccgcctcc
    19561 gggtgcgtat caaacgccgc catcaccagc gctaactcat ccacagtaaa gccatgtttc
    19621 gcatcatcta cgcccagacc aaactgttcc tgcatcacgg catataacga cgccacatct
    19681 ggtagatttc gctgctcaac tgcgtgccca ttttcataat gggtaaaatg aaaattggtc
    19741 agcgttagct tgccaccgtc cggcaaatgg cgacacatca gcaaatgatg gcgaaaatgc
    19801 gactgcggcc agtgcgccga ccagaaattg cctatcacat aatcgctttg ttgctgctcg
    19861 cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg caatacccaa
    19921 tcatcaccct cttgcaacaa ccgatactct ccgtgtggcg tggtttgcat gatatccggc
    19981 attaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc aatccatttt
    20041 tccccttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc tggcggattt
    20101 gataacacta cgcgacctaa caagctgcga acgttaaacc ccagctcgcg taacacccgc
    20161 tcaaacacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac cagtttctct
    20221 tccagcgatt gatcatcaag ctgtatttcc ctcggcagca aaacgtcgag attttcaaac
    20281 ggaatggtgc aattgtgttt caggtgcaat gcacgcagcg tatcaatatt ggccgcagca
    20341 gctcccaacc agttaatacg ggcaaaataa tgattcagaa tgggcgtcat caggcgtttc
    20401 ccttagtggt tttctcaact tataacctgg aatagacgcc gcactggcta ttttgtgcga
    20461 tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta cttattgcgt
    20521 tacgagctac gcttttaaaa cacccttcat agcgaggcga tatcgtgagc caattcattc
    20581 ccgtcgaatt acaccatgcc agctgtctgt taaatcatgg cccaactgtc atgatcacca
    20641 gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtcg atgcccgtag
    20701 agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga
    20761 ttgaaggtaa cggtaaattt ggcatcgtta tcccgggcgt tgcggcgact aactggacgt
    20821 gggcggtggg aagtgtgtcg ggtcgtgaag aagataagtt taattgctac ggcattccgg
    20881 tagtgagagg cccggtgctt ggtttgccgc tggtcgagga aaaatgtctg gcgtggatgg
    20941 agtgtcgcct gctacctgcg acttccgcgc aagaaaaata cgatacgcta tttggcgaag
    21001 tggtatctgc agcggcagac gcacgagtat ttgtcgaagg ccgctggcag tttgatgatg
    21061 ataagctcaa tacgttgcat catttaggtg ctggcatgtt tgttaccagt ggtaagcgag
    21121 ttacggcagg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg
    21181 ggcgcgattt cggccagggc aagattacga aagcccgctc cccgcaagga ctgacgcgag
    21241 atagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    21301 atcattcgtt aaaatattaa tagcgatgtg ccgtatccct gaaaataatt ctgcggcgtt
    21361 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc agtgcagctt
    21421 attctccacg tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc
    21481 agaactgata taatatctga ccgtcatttc tggctctttc ttttgttctg ctattatgga
    21541 ccgaaaggag actgccacgc ataatttctt cagtcctttc cattcgaacg tgaaatcaat
    21601 aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt
    21661 ttcactaatg gcgtaactgt catgttctgg attatttaat tctttcagtg gaaatttttc
    21721 ctcaaaggct ttattaagcc gcccctgatt tccttttaca gcgaataaat aatcaccgcc
    21781 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat
    21841 ttttccttta atatccagca tgttaagaag ttcagggata ggtgtaattt cattggattt
    21901 ctcatccgtc ctgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgac
    21961 atgaatcgct cccttgcggc gactcttgtc ataagaatgc cggagtgttt ttccatcaat
    22021 tgcgatgacg tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc
    22081 gtgaaatttt gcaggactga tacaggatac aactctggca atggtatcgt gaacaggaat
    22141 accattatca aaatcaccat attgcttcaa aaaatcgaga tgtgtttccc caaaatcctc
    22201 tatatcttcc cagccttctg cacccgaaat aacggcacaa atagtcaaca gtagaatatc
    22261 cgataattta tgttccactt tccaggcttg tctgtaatcg gggataatag aaatatgttc
    22321 catcaatttt ttaagttcca ttttgttctc cttaattatg taaggagtat ttgatcatgt
//
LOCUS       DAJOTB010000076        21702 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJOTB010000076 DAJOTB010000000
VERSION     DAJOTB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.076"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..498
                     /locus_tag="OHN28_002799"
     CDS             163..498
                     /locus_tag="OHN28_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCQ2298169.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            658..1101
                     /gene="yjdN"
                     /locus_tag="OHN28_002800"
     CDS             658..1101
                     /gene="yjdN"
                     /locus_tag="OHN28_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCQ2298170.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1234..2022
                     /gene="phnC"
                     /locus_tag="OHN28_002801"
     CDS             1234..2022
                     /gene="phnC"
                     /locus_tag="OHN28_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298171.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2047..3063
                     /gene="phnD"
                     /locus_tag="OHN28_002802"
     CDS             2047..3063
                     /gene="phnD"
                     /locus_tag="OHN28_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2298172.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3168..3947
                     /gene="phnE"
                     /locus_tag="OHN28_002803"
     CDS             3168..3947
                     /gene="phnE"
                     /locus_tag="OHN28_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCQ2298173.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3968..4693
                     /gene="phnF"
                     /locus_tag="OHN28_002804"
     CDS             3968..4693
                     /gene="phnF"
                     /locus_tag="OHN28_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCQ2298174.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4694..5146
                     /gene="phnG"
                     /locus_tag="OHN28_002805"
     CDS             4694..5146
                     /gene="phnG"
                     /locus_tag="OHN28_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCQ2298175.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5143..5727
                     /gene="phnH"
                     /locus_tag="OHN28_002806"
     CDS             5143..5727
                     /gene="phnH"
                     /locus_tag="OHN28_002806"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCQ2298176.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5727..6791
                     /gene="phnI"
                     /locus_tag="OHN28_002807"
     CDS             5727..6791
                     /gene="phnI"
                     /locus_tag="OHN28_002807"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCQ2298177.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFPLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6784..7629
                     /gene="phnJ"
                     /locus_tag="OHN28_002808"
     CDS             6784..7629
                     /gene="phnJ"
                     /locus_tag="OHN28_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCQ2298178.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7626..8384
                     /gene="phnK"
                     /locus_tag="OHN28_002809"
     CDS             7626..8384
                     /gene="phnK"
                     /locus_tag="OHN28_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCQ2298179.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8495..9175
                     /gene="phnL"
                     /locus_tag="OHN28_002810"
     CDS             8495..9175
                     /gene="phnL"
                     /locus_tag="OHN28_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCQ2298180.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9172..10308
                     /gene="phnM"
                     /locus_tag="OHN28_002811"
     CDS             9172..10308
                     /gene="phnM"
                     /locus_tag="OHN28_002811"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCQ2298181.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGKIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVEREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10308..10865
                     /gene="phnN"
                     /locus_tag="OHN28_002812"
     CDS             10308..10865
                     /gene="phnN"
                     /locus_tag="OHN28_002812"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCQ2298182.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10852..11286
                     /gene="phnO"
                     /locus_tag="OHN28_002813"
     CDS             10852..11286
                     /gene="phnO"
                     /locus_tag="OHN28_002813"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCQ2298183.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11288..12046
                     /gene="phnP"
                     /locus_tag="OHN28_002814"
     CDS             11288..12046
                     /gene="phnP"
                     /locus_tag="OHN28_002814"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCQ2298184.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12074..12430)
                     /locus_tag="OHN28_002815"
     CDS             complement(12074..12430)
                     /locus_tag="OHN28_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298185.1"
                     /translation="MKRFPLFLLFTLFTLFTLFTLFTLFTVPAQADIIDDTIGNIQQA
                     INDAYNPDRGRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQER
                     QQLEDEERRMEDEYGR"
     gene            complement(12499..12948)
                     /gene="rpiB"
                     /locus_tag="OHN28_002816"
     CDS             complement(12499..12948)
                     /gene="rpiB"
                     /locus_tag="OHN28_002816"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCQ2298186.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13307..14197
                     /gene="alsR"
                     /locus_tag="OHN28_002817"
                     /gene_synonym="rpiR"
     CDS             13307..14197
                     /gene="alsR"
                     /locus_tag="OHN28_002817"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCQ2298187.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14256..15191
                     /gene="alsB"
                     /locus_tag="OHN28_002818"
     CDS             14256..15191
                     /gene="alsB"
                     /locus_tag="OHN28_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCQ2298188.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATETFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15319..16851
                     /gene="alsA"
                     /locus_tag="OHN28_002819"
     CDS             15319..16851
                     /gene="alsA"
                     /locus_tag="OHN28_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HCQ2298189.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16830..17810
                     /gene="alsC"
                     /locus_tag="OHN28_002820"
     CDS             16830..17810
                     /gene="alsC"
                     /locus_tag="OHN28_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCQ2298190.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17821..18516
                     /gene="alsE"
                     /locus_tag="OHN28_002821"
     CDS             17821..18516
                     /gene="alsE"
                     /locus_tag="OHN28_002821"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCQ2298191.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     RERKDLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18500..19429
                     /gene="alsK"
                     /locus_tag="OHN28_002822"
     CDS             18500..19429
                     /gene="alsK"
                     /locus_tag="OHN28_002822"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCQ2298192.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19702..21687
                     /gene="yjcS"
                     /locus_tag="OHN28_002823"
     CDS             19702..21687
                     /gene="yjcS"
                     /locus_tag="OHN28_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCQ2298193.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5194 a         5985 c         5781 g         4742 t
ORIGIN      
        1 aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcatct aaaatcagca
       61 gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg caatctgccg
      121 ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt accacactgc
      181 ccaaaatgca actccgaata cacctacgaa gataacggca tgtacatctg cccggaatgt
      241 gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat cgttaaagat
      301 gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga tctgaaagtg
      361 aaaggtagct cttcgatgct gaaaattggc accaaagtaa aaaacatccg cctggttgaa
      421 ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact gaagtctgag
      481 tttgtgaaaa agaactgatt gtattgtgat cggtaggccg gataaggcgc tcgcgccgca
      541 tccggcaacg gtggcgactg cctgatgcga cgctgaccgc gtcttatcag gcttccctct
      601 catctgtata aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg
      661 ccgttaagtc cctacctctc ttttgccggt aactgttccg acgcgattgc ctattatcaa
      721 cgtacgttgg gcgcggaact gctctataaa atcagcttcg gcgaaatgcc aaaatcaacg
      781 caggacagcg ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgcccac
      841 gccaatgtgc gcattgcagg aagcgacatc atgatgagcg atgctatacc gtcaggaaaa
      901 gccagctact ccggctttac gctggtgctc gattctcaac aggtggaaga aggaaaacgc
      961 tggtttgata accttgccgc caacggaaaa atcgaaatgg cctggcagga aacgttctgg
     1021 gcgcatggct ttggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcatc
     1081 aaacaacagc caacgcaata acccgccggg aggcccgccc tcccgcactg tcatcgaatt
     1141 cccgttaact cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc
     1201 acgatggagc cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag
     1261 ctcgccaaaa ccttcaatca gcatcaggcg ctgcacgcgg ttgatctgaa cattcatcat
     1321 ggtgaaatgg tggctctgct tgggccgtcg ggttccggaa aatccaccct tttacgtcac
     1381 ttaagcggtt tgattaccgg cgataaatct gtcggtagcc atatcgagct gctgggccgc
     1441 acagtccagc gcgaaggccg cctggcccgc gatatccgca aaagccgcgc caacaccggc
     1501 tacatctttc aacaattcaa cctggtgaat cgcctgagcg tactggagaa cgtgctgatt
     1561 ggcgcgctcg gcagcacgcc attctggcga acctgtttta gctacttcac ccgcgagcag
     1621 aaacagcgcg cgttacaggc gctgacccgc gttggcatgg tgcactttgc ccatcaacgc
     1681 gtttccacac tctccggcgg ccagcaacag cgtgtggcga ttgcccgcgc gctgatgcag
     1741 caggcgaagg tcattctcgc tgacgaaccc atcgcctcgc tggacccgga atcggcacgc
     1801 atcgtgatgg acaccctgcg cgacatcaac cagaacgacg gcattaccgt ggtcgtcacg
     1861 ctgcatcagg tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg
     1921 cacgtcttct acgacggctg cagccaacag tttgataacg aacgttttga ccatctctac
     1981 cgcagcatta accgcgtcga agagaacgcg aaagctgcct gacatcccca tcattgagga
     2041 aaacgaatga acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc
     2101 accctgttaa atccggcata cgccgaagag caggaaaagg cgctgaattt cggcattatt
     2161 tcaacggaat cacagcaaaa cctgaaaccg caatggacgc cgttcttaca ggatatggag
     2221 aagaagctgg gcgtaaaggt caacgccttc tttgccccgg actacgcggg cattatccag
     2281 gggatgcgct tcaataaagt ggatatcgcc tggtacggca acctgtcggc gatggaagcg
     2341 gtggatcgcg ccaacggtca ggtcttcgcc cagacggtcg cggcggatgg atcgccgggt
     2401 tactggagcg tgttgatcgt caacaaagat agcccgatca acaacctgaa cgatctgctg
     2461 gcgaagcgga aagatctcac cttcggcaat ggcgatccta actctacctc cggcttcctc
     2521 gtccccggtt actacgtctt cgctaaaaac aatatctccg ccagcgactt taagcgcacc
     2581 gtcaacgccg ggcatgaaac caacgccctc gccgtcgcta acaagcaagt ggatgtcgcc
     2641 accaacaaca ccgaaaacct cgacaagctg aaaacctccg cgccggagaa gctgaaagaa
     2701 ctgaaggtga tctggaaatc accgctgatc cctggcgatc cgatcgtctg gcgcaaaaat
     2761 ctttccgaaa ccactaaaga caagatctac gacttcttta tgaattacgg caaaacgccg
     2821 gaagagaaag cggtgctgga gcgcctgggc tgggcgccgt tccgcgcctc cagcgacttg
     2881 caactggtgc cgattcgcca gctcgcactg tttaaagaga tgcaaagcgt gaaggacaac
     2941 aaagggctga acgaacagga caagctggca aaaaccaccg ccattcaggc gcaactggat
     3001 gatttggacc gcctgaacaa cgcgttaagc gcaatgagtt cggtgagtaa agcggtgcag
     3061 taaatcgtag gtcggataag acgcccggcg tcgcatccga caatgtgcag acgttgatgc
     3121 cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg caaaccatca
     3181 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccgtt gttatcgccg
     3241 tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt aaagatggcg
     3301 gcaacatggc gacgttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
     3361 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcggtgg
     3421 ttctctccat ccccttcggc ctgatgagcg ctgaaaacct ggtaccgtgg tgggtctacc
     3481 aacctgtacg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
     3541 tgttcgtggt cgccgtcggc ctcggcccgt tcgctggcgt gctggcgctg tttatccaca
     3601 ccaccggcgt gctctccaag ctgctttccg aagcggtgga agcgatcgaa cccggcccgg
     3661 tggagggcat tcgcgccacc ggtgccaaca agctggaaga gatcctttac ggcgtactac
     3721 cgcaggtcat gccgctgctg atttcctact ctctctatcg cttcgaatcc aacgtccgct
     3781 cggcgaccgt cgtcggtatg gttggcgcgg gcgggatcgg cgtcacgctg tgggaagcga
     3841 ttcgcggttt ccagttccag caaacctgcg ccctgatggt gctcatcatc gtcacggtca
     3901 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gcgaggcatt
     3961 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
     4021 cgcaaaactt gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga
     4081 gcagcaactg gcggcgcgct ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca
     4141 actggtggaa aaaggctggg tacagcgccg tcagggcgtc ggcgtactgg tgctgatgcg
     4201 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
     4261 cagccatccc accagcgaaa aattgctatc ggtgctgcgc cccgcctccg gtcacgtcgc
     4321 tgacgcgctg gggataaccg agggggagaa cgtcatccac ctgcgcaccc tgcgtcgggt
     4381 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
     4441 gctgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa cggggattac
     4501 gctacgacgc agtcagacgc ggatcagcgc ccgtcgcgcc caggccaaag agtgccagcg
     4561 tcttgaaatc ccgaatctgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
     4621 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
     4681 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtgctggc
     4741 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca acgccgatta
     4801 tgaggtgatc cgcgccgctg aaactggcct ggtgcagatt caggcgcgaa tgggcggcac
     4861 cggcgaacgc ttttttgctg gcgacgccac gctcacccgc gccgccgtgc gtctgactga
     4921 cggcacgctc ggctatagct gggtgctggg gcgcgacaaa cagcacgccg aacgttgtgc
     4981 gctgattgat gcgctgatgc agcaaaaccg ccactttcaa aacttatcag aaacccttat
     5041 cgccccgctg gacgctgacc gtatggcacg cattgccgcg cgccaggccg aagtgaacgc
     5101 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
     5161 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
     5221 gagccgggcg tgattgtcgc cctgcatcag ctcaagcgcg gctggcagcc gctgaatatc
     5281 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccgcg
     5341 ccattgagca acgatatcgt caatcagagc ctgcgttttc ataccaacgc gtcgctggtt
     5401 aatcagccgg aacaggcgac cttcgcggta acagatgagg cgatctccag cgagcagctc
     5461 aacgcccttt ccaccggcac tgccgttgca ccagaagcgg gcgcaacgct gattttacag
     5521 gtcaccagcc tgagcggcgg gcgtatgttg cgcctcaccg gcgcaggtat tgccgaagaa
     5581 cggatgatcg ccccgcaact gccaaagtgc attcttcatg aacttaccga gcgcccgcac
     5641 ccgttcccgc tcggcatcga cctgatcctg acctgcggcg agcgcctgct ggctattccg
     5701 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
     5761 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgatactgat ttgcctgaac
     5821 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt agatcgcgtg atgaccgaag
     5881 gcggtattgc cgaccgcgag ctggccgcgc tggcgctgaa acaggccagc ggcgataacg
     5941 tcgaagcgat ttttctgttg cgcgcctacc gcaccacgct ggcgaagctg gcggtcagtg
     6001 agccgctcga caccaccggg atgcgtctcg aacggcgtat ttcagcaatt tataaagaca
     6061 ttcccggcgg ccaactgctt ggccccacct acgactacac ccatcgcctg ctcgatttcc
     6121 ccctgctggc aaacggcgaa acgccaacgc tgaccaccgc tgacagcgaa cagcaaccgt
     6181 cgccacacgt cttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaagatagcg
     6241 gcgcacaacc ggacgacatc acccgcacgc cgccggttta tccctgttcg cgttcttccc
     6301 gtctacaaca attaatgcgc ggtgacgaag gctatttgtt ggcgctggcc tactccaccc
     6361 aacgcggtta cgggcgcaat cacccgttcg caggcgaaat tcgcagtggc tatatcgata
     6421 tatttattgt gccggaagag ctgggatttg cggtgaacgt cggcgaattg ttgatgaccg
     6481 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga accaccgcat tttacacgcg
     6541 gctatgggct ggtgttcggc atgagcgagc gcaaagcaat ggcgatggcg ctggttgacc
     6601 gtgcgttgca ggcaccggaa tacggcgagc acgcaacagg tccggcacag gatgaagagt
     6661 tcgtgctggc ccatgccgac aacgtcgaag ccgcaggctt tgtttcgcat ctcaaactcc
     6721 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
     6781 accatggcta atctgagcgg ctacaacttt gcttacctcg acgagcagac caaacgcatg
     6841 atccgccgcg ctatcttaaa agcggtggca atccccggtt atcaggtgcc gtttggcgga
     6901 cgcgagatgc cgatgcccta cggctgggga accggcggca ttcagctcac cgccagcgtg
     6961 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggcg cagacgacac caccaacgcc
     7021 gtgtcgattc gtaacttctt taagcgcgtg accggggtga acaccaccga gcgcacggac
     7081 gatgcgacac ttatccagac gcgtcaccgc atacccgaaa cgccgctgac cgaagatcag
     7141 atagttatct tccaggtgcc gatccccgaa ccgctgcgct ttattgagcc gcgtgagacg
     7201 gaaacgcgca ccatgcacgc gctggaggag tacggcgtga tgcaggtgaa gctgtatgaa
     7261 gatatcgccc gcttcggtca tatcgccacc acctacgcct atccggtgaa ggtgaacggg
     7321 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
     7381 cccgccctgc aactgtttgg cgcggggcgc gagaagcgca tctatgccgt gccaccgttt
     7441 acccgcgtgg aaagcctcga tttcgatgat catccgttca ccgtccagca gtgggatgaa
     7501 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
     7561 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggccaaa
     7621 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttat gccccgggca
     7681 aaggcttcag cgatgtctct tttgatttat ggccgggaga agttctgggc attgttgggg
     7741 agtccggttc cgggaagacc acgctactga aatcaatttc cgcccgcctg acaccacagc
     7801 aaggggaaat tcgctacgag aaccgttcgc tgtacgcgat gagcgaggcc gaccgccgtc
     7861 gcctgctgcg caccgaatgg ggtgtggtgc atcagcatcc gcttgacggc ctgcgccgcc
     7921 aggtgtcggc aggcggcaat atcggcgaac ggttgatggc aaccggagcg cgtcattacg
     7981 gcgatattcg cgccaccgcg cagaaatggc tggaagaggt ggagattccc gccaaccgga
     8041 tcgacgacct gccgaccacc ttttccggcg gcatgcagca acgtttgcag attgcccgca
     8101 acctggtgac gcatccgaag ctggtgttta tggatgaacc cactggcggg ctggatgtgt
     8161 cggtacaggc gcgcctgctg gacctgctcc gcggtctggt gatggagctt aacctggcgg
     8221 tagtgattgt cacccacgat ttaggcgtcg cccgtctgct ggcggaccgt ttgctggtaa
     8281 tgaagcaagg gaaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcacc
     8341 atccgtatac ccagttgctg gtgtcatcgg ttttgcagaa ttgagctggt gccggatgcg
     8401 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcatcag
     8461 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
     8521 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgcct gtattacaag gcgcctcact
     8581 caccgtcaac gccggcgaat gcgtggtact ccacggccat tccggcagtg gaaaatcaac
     8641 tctgctacgc tcgctgtatg ccaactatct acccgacgaa gggcaaatcc agatcaaaca
     8701 cggtgatgaa tgggtcgacc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
     8761 aaccactgtc ggctgggtaa gccagtttct gcgcgtcatc ccacgtattt cggcactgga
     8821 agtggtgatg cagccgctgc tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
     8881 cgcgcgtctc ctcacccgcc tgaacgtgcc tgaacgcctg tggcaccttg cgccatcgac
     8941 cttctccggt ggcgaacagc agcgtgtcaa catcgcccgt ggctttatcg tcgactaccc
     9001 cattctgctg ctcgacgaac ctaccgcctc gctggacgct aaaaacagcg ccgccgtagt
     9061 ggaactgatc cgcgaagcca aagcccgcgg cgcggccatc gtcggtatct tccatgacga
     9121 aaccgtacgc gagcaagtcg ccgaccgcct gcacccaatg ggagtctctt catgattatc
     9181 aataacgtca aactggtgct ggaaaacgag gtggtacacg gttctctgga gatgcaggac
     9241 ggcaaaatcc gcgcctttgc cgaaagccag agtcacctgt cagaagcaat ggacggcgaa
     9301 ggcggctggc tgctaccggg gctgattgag ctgcataccg ataatctgga taaattcttc
     9361 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
     9421 atggtggcga gcggcatcac caccgtgctg gacgccgtgg cgattggtga tgtacgcgac
     9481 ggcggcgatc ggctggagaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
     9541 cgcggcgtca accgtgccga gcaccgtctg cacctacgct gcgaactgcc gcatcacacc
     9601 acgctgccgc tgtttgaaaa actggtcgag cgagaacccg tgacgctggt gtcgctgatg
     9661 gatcactcac cgggccagcg ccagttcgcc aaccgcgaaa aatatcgtga atattatcag
     9721 ggcaaatact cgctcaccga tgcgcagatg caacagtacg aagaggagca actggcgctc
     9781 gccgcacgct ggtcacagcc gaatcgcgaa tccatcgccg caatgtgccg cgcgcgacac
     9841 attgcgctcg ccagccacga tgacgccacg cacgcccacg tagcccaatc ccatcagctt
     9901 ggcagcgtga tcgccgaatt tcctaccaca ttcgaagcgg cagaagcctc gcgcaagcac
     9961 ggcatgaacg tactgatggg cgcgccgaat atcgtgcgcg gcggttcgca ctccggcaac
    10021 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    10081 cccgccagcc tgctggatgc ggcgtttcgc gtggccgatg accagagcaa ccgctttacg
    10141 ctaccgcagg cggtgaggct ggtgaccaaa aacccggccc aggcactgaa tctccaggat
    10201 cgcggggtga ttggcgaggg caaacgcgcc gacctggtgc tggcgcatcg caagggcaat
    10261 cacattcata ttgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg acgggaaaac
    10321 tgatttggtt aatgggtccg tccggctccg ggaaagacag cctgctggcg gaactccgcc
    10381 agcgggaaca aacccagcta ctggtagcgc atcgctacat cacgcgtgat gccagcgccg
    10441 gaagtgaaaa tcatatcgcc ttaagcgagc gggagttttt tacccgcgcg gggcaaaatc
    10501 tgttggcctt gagctggcat gctaatggtt tgtattacgg tgtcggcatt gagatcgacc
    10561 tctggctgca cgccggattc gacgtcgtgg ttaacggctc acgcgcccac ctgccgcagg
    10621 cgcgggcacg ttatcaatcg gcactgctgc ccatctgttt acaggtttcg ccggagatcc
    10681 tgcgccagcg cctggaaaac cgtggccgcg aaaacgccag tgaaattaac gcccggctgg
    10741 cgcgcgccgc ccgctatact cctcaggatt gtcttacgct caataatgac ggcagcctgc
    10801 gccagtcggt cgacaagctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    10861 tgtgagcttc gcccggctac gcagtacgac accgacgcag tttacgcgct gatttgtgag
    10921 ctaaaacagg cggagtttga ccaccacgcg tttcgcgtgg ggtttaacgc caatctgcgc
    10981 gatccgaaca tgcgctacca tctggcgctg ctggatggcg aagttgtcgg catgatcggc
    11041 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggagttggtg
    11101 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    11161 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    11221 gcgcaccgtt tctatctgcg cgaaggctac gagcagagcc atttccgctt caccaaagcg
    11281 ctgtaacatg agcctgatcc tcacgctcac cggcactggc ggcgcacagg gcgttcccgc
    11341 atggggctgt gagtgcgcgg cctgcgccag agcgcggcgc tcaccgcagt atcgccgcca
    11401 gccatgcagc ggcgtcgtga agtttaacga cgcgatcacc ctgatcgacg ccgggctgca
    11461 cgatctcgcc gatcgctggt cgcccggatc gttccagcag tttttgctga cgcactacca
    11521 tatggatcac gtgcaggggc tgtttccgct gcgctggggc gtgggcgaca caatcccggt
    11581 ctacggtcca ccggatgagc agggctgcga cgatctgttt aaacatccgg gcctgcttga
    11641 tttcagccac acggtggagc cgtttgtggt gtttgatttg caggggttgc aggtcacgcc
    11701 gctgccgctt aaccattcga aactcacttt cggctattta ctggaaacgg cgcacagccg
    11761 ggtggcgtgg ctatctgaca ccgcaggcct gccggaaaaa acgctgaaat ttttacgcaa
    11821 taatcagccg caggtaatgg tgattgattg cagccacccg ccgcgcgagg atgcaccgcg
    11881 taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct cgccacgggt
    11941 gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaatg cactgccgtc
    12001 ggggtttgag gcggggtttg atgggatgga gattgaggtg gagtgaggga aaatacccgc
    12061 gccggcccct acctcaacgc ccatactcat cttccattct ccgctcttca tcttccagtt
    12121 gctgacgctc ctgatcaagc tggcgctggc gttcgtccag ctgcctgcgg cgatcttcaa
    12181 actggcggcg gcggtcatca tattgtctgc gccgatcgtc gctcacttca cgctgccagc
    12241 cgtcgtcgcg cgaatcttca tagtctcgcc cacggtcagg gttataagcg tcgttaatcg
    12301 cctgctgaat attgccaatg gtgtcatcga taatatcggc ctgggccgga acggtgaaca
    12361 gcgtgaacag cgtgaacagc gtgaacagcg tgaacagggt gaataaaaga aatagcggaa
    12421 agcgtttcat tagccaacct caaaaagaaa ctctatccac attagtcatt actcatccat
    12481 gcaagtagtg gatgaatctc aatttctccg ctgctctatt gccgtaatcg cctccacgcg
    12541 ttgttgatga cgaccgcctt cgtactgtgc gcccagccac gcatcgacaa tcatttttgc
    12601 cagttcgagg ccaactactc gtgaaccaaa agccagcacg ttggtgtcgt tatgctgccg
    12661 cgaaagttgc gcggaataag gttcgctaca aacgaccgcg cgaattccgg caaacttgtt
    12721 cgccgctatc gaaataccga cgccagtgcc acaaatcaaa atcccgccat cagcctctcc
    12781 gccagcaacg gccagtgcga cttgactggc gtaatgcgga taatcagtac gctctgacga
    12841 ccaggttcct ttatcaatca cttcaacgcc acgctcaact aaatgtgcca ctatttcatg
    12901 ttttaaaatg aaaccgacat gatcacagcc aaatgcaatc tttttcattc atcttccccg
    12961 ctcgcgcaaa acttcacaat aaaaaaacat ttgtgattta aatgaattaa aaacacccaa
    13021 aatcgatgaa atcagccttc ccgatctgtt ttacagagtt acatctggca gcgcaatagc
    13081 gttaaagaca ctcactatcc ggcaacatct atcatctaat aaaccagaaa aacaaataac
    13141 atcatgtttt taaagtgatt aaatgaaata aaattttaag ccactctcca ttattcacaa
    13201 taaaacaaac tttataaatt ttattttttt gtgaagttgc cagcatcttt tctgttcttg
    13261 ctgtggtgat atagtggcgt cttcaattca aggacaagag aacgtgatga gccagtcaga
    13321 gtttgattca gcgcttccga acggtatcgg gttagcgcct tacctgcgaa tgaagcagga
    13381 aggaatgaca gaaaacgaaa gccgcatcgt ggagtggtta ctcaaacccg gtaacctgag
    13441 ttgtgcaccc gcaattaaag atgtcgcaga agctctggcg gtatctgaag cgatgatcgt
    13501 taaggtatca aagctgctgg ggtttagcgg ctttcgtaac ttacgcagtg cgctggaaga
    13561 ttatttttct cagtcagaac aagtattgcc gtccgagttg gcttttgatg aagcgccgca
    13621 ggatgtggtg aataaggtat ttaacatcac tttacgcacc attatggaag gtcagtcgat
    13681 cgtcaacgtt gatgagatcc accgtgccgc ccgctttttc tatcaggcca gacagcggga
    13741 tttgtacggt gccggaggat caaatgcaat ctgtgctgat gtacagcaca agtttttacg
    13801 cattggcgta cgctgccagg cctatccgga cgctcacatc atgatgatgt ctgcttcgtt
    13861 gttacaggaa ggagatgtgg tgctggtggt gacccattcc gggcgaacca gtgatgtaaa
    13921 agcagctgta gaactggcaa aaaagaacgg ggcaaagatt atttgtataa cgcatagcta
    13981 ccattcaccg atagcgaagc tggccgatta tattatttgc tcaccggccc cggaaacacc
    14041 gttattaggt cgtaatgcct cggcaagaat attacaacta actttgctgg acgcgttttt
    14101 tgtctctgtc gcccagctca acattgaaca agctaatatt aatatgcaaa aaaccggcgc
    14161 aattgttgat ttcttctcac caggcgcgct gaaataagaa aaaggtaatt ccgccatcgc
    14221 ggagttacct atccccttac cgtgagaaca tgattatgaa taaatatctg aaatatttca
    14281 gcggcacact cgtgggctta atgttgtcaa ccagcgcttt tgctgccgcc gaatatgccg
    14341 tcgtattgaa aacactctcc aacccatttt gggtagatat gaaaaaaggc attgaagatg
    14401 aagcaaaaac gctgggcgtc agcgttgata tttttgcctc tccttcagaa ggcgattttc
    14461 aatctcaatt gcagttattt gaagatctca gtaataaaaa ttacaaaggt atcgccttcg
    14521 caccattatc ctcagtgaac ctggtcatgc ctgtcgcccg cgcatggaaa aaaggcattt
    14581 atctggtcaa tctcgatgaa aaaatcgaca tggataatct gaaaaaagca ggcggtaatg
    14641 tggaaggttt tgtcaccacc gataacgttg ccgtcggggc gaaaggcgcg tcattcatta
    14701 ttgacaagct gggtgccgaa gggggtgaag ttgcaattat tgaaggtaaa gccggtaacg
    14761 cctccggtga agcgcgtcgt aatggtgcca ccgaaacctt caaaaaagca agccagatca
    14821 agcttgtcgc cagccagcct gccgactggg accgcattaa agcactggat gtcgccacta
    14881 acgtgttgca acgtaatccg aatattaaag cgatctattg cgcgaatgac acgatggcaa
    14941 tgggtgttgc tcaggctgtc gcaaacgccg gaaaaacggg aaaagtgctg gtcgtcggta
    15001 cagatggcat tccggaagcc cgcaaaatgg tggaagccgg acaaatgacc gcgacggttg
    15061 cccagaaccc ggcggatatc ggcgctacgg gtctaaagct gatggttgac gctgaaaaat
    15121 ccggcaaggt tatcccgctg gataaagcac cggaatttaa actggtcgat tcaatcctgg
    15181 tcactcaata aaactaaatg atgccggata gtgatgctaa tgaagtgtct tatccggcca
    15241 acaaaataac ctgaacgtag acctgataag tgtagcccat caggcaataa tacgatattt
    15301 cgtgcagaga ggttatttat ggccacgcca tatatatcga tggcggggat cggcaagtcc
    15361 tttggtccgg ttcacgcatt aaagtcggtt aatttaacgg tttatcctgg tgaaatacat
    15421 gcattactag gagaaaatgg cgcgggtaaa tccacgctaa tgaaagtgtt atccggaata
    15481 catgagccaa ccaaaggcac cattaccatt aataacatta actataacaa actggatcat
    15541 aaattagcgg cacaactcgg tatcgggatt atttatcagg aactcagcgt tattgatgaa
    15601 ttaaccgtac tggaaaattt atatattggt cgtcatctga cgaaaaaaat ctgcggtgtc
    15661 aatattatcg actggcgaga aatgcgtgtc cgcgccgcaa tgatgttatt acgcgtgggc
    15721 ttgaaagttg atttagatga gaaagtggcg aacttatcta tcagccacaa gcagatgctg
    15781 gaaattgcca aaacgctgat gctcgacgcc aaagtcatca tcatggatga acccacctcc
    15841 tcactcacca ataaagaggt ggactatctg tttctgatca tgaatcagtt gcgtaaggag
    15901 ggtacggcta tcgtctatat ctcgcataaa ttggcggaaa ttcgccgtat ttgcgaccgc
    15961 tatacggtga tgaaagacgg cagcagcgtt tgcagcggca tggtgagcga tgtgtcaaat
    16021 gacgatatcg tccgtctgat ggtaggccgc gaactgcaaa accgttttaa cgcgatgaag
    16081 gagaatgtca gcaaccttgc gcacgatacg gtttttgagg tgcgcaacgt caccagccgt
    16141 gacagaaaaa aggtccggga tatctcattt agcgtctgcc ggggagaaat attaggcttt
    16201 gccggactgg tcggttccgg acgtactgaa ctgatgaact gcctgtttgg cgttgataaa
    16261 cgcgctggcg gagaaatccg tcttaatggc aaagatatct ctccacgttc tcccctggat
    16321 gccgtgaaaa aagggatggc ttacatcact gaaagccgcc gggataacgg ctttttcccc
    16381 aacttttcta tcgctcagaa catggctatc agccgcagtc tgaaagacgg cggctataaa
    16441 ggcgcgatgg ggttgtttca tgaagttgac gagcaacgta ccgctgaaaa tcaacgcgaa
    16501 ctgctggcgc tgaaatgtca ttcggtaaac cagaacatca ctgaactctc cggcggtaac
    16561 cagcagaaag tcctgatctc caaatggctg tgctgtcgcc cggaagtgat cattttcgat
    16621 gaacctaccc gtggcatcga cgttggcgcg aaagccgaaa tttacaaagt gatgcgccaa
    16681 ctggcggacg acggaaaagt catcctgatg gtgtcatctg aactacctga aattatcacc
    16741 gtctgcgacc gcatcgccgt gttctgcgaa ggacgactga cgcaaatcct gacgaatcgc
    16801 gatgacatga gcgaagagga gattatggca tgggctttac cacaagagta aaaagcgaag
    16861 cgagcgagaa gaaaccgttc aactttgcgc tgttctggga taaatacggt acttttttta
    16921 tcctggcgat catcgtcgcc atttttggtt cgctgtcgtc agaatatttt ctgaccacca
    16981 ataatattac ccagattttt gtgcaaagct ccgtgacggt actgatcggc atgggggaat
    17041 tcttcgctat cctggtcgcc ggtatcgacc tttcggttgg cgcgattctg gcgctttccg
    17101 gtatggtgac cgctaaactg atgttggcag gtattgatcc gtttctcgcg gcgctaattg
    17161 gcggtgtact ggttggcggc gcactggggg cgatcaacgg ctgcctggtc aactggacgg
    17221 gactacaccc gttcattatc acccttggca ccaacgctat tttccgtggg atcacgctgg
    17281 tgatctccga tgccaactcg gtatacggct tctcatttga cttcgtgaac ttctttgccg
    17341 ccagcgtaat tgggatacct gtccccgtta tcttctcgct aattgtcgcg ctcatccttt
    17401 ggtttctgac aacgcgtatg cggctcgggc gcaacatcta cgcactgggc ggcaacaaaa
    17461 actcggcgtt ctattccggg attgacgtga aattccacat cctggtggtg tttatcatct
    17521 ccggtgtttg tgcaggtctg gcaggcgtcg tctcaactgc acgactcggt gccgcagaac
    17581 cgcttgccgg tatgggtttt gaaacctatg ccattgccag cgccatcatt ggcggcacca
    17641 gtttcttcgg cggcaagggg cgcattttct ctgtggtgat tggcgggttg atcatcggca
    17701 ccatcaacaa cggtctgaat attttgcagg tacaaaccta ttaccaactg gtggtgatgg
    17761 gcggattaat tatcgcggct gtcgcccttg accgtcttat cagtaagtaa ggaattgaac
    17821 atgaaaatct ccccctcgtt aatgtgtatg gatctgctga aatttaaaga acagatcgaa
    17881 tttatcgaca gccatgccga ttactttcac atcgatatca tggacggcca ctttgtgccg
    17941 aatctgacgc tctcaccgtt cttcgtaagc caggtaaaaa aactggcgag taaaccgctc
    18001 gactgccatc tgatggtaac gcgtccgcag gattacattg ctcaactggc acgtgcggga
    18061 gctgatttca tcactttgca tccggaaact atcaacggtc aggcattccg cctgattgat
    18121 gaaatccgcc gtcatggcat gaaagtgggg ctgattctta atccggaaac gccggttgag
    18181 gccatgaaat actatatcca taaggctgat aaaattacgg tcatgactgt cgatcccggt
    18241 tttgccggac aacccttcat tcctgaaatg ctggataaag ttgccgaact gaaggcatgg
    18301 cgtgaacgaa aagatctgga gtacgaaatt gaggtggacg gttcctgcaa ccaggcaact
    18361 tacgaaaaac taatggcggc aggggcggat gtctttatcg tcggcacttc cggcctgttt
    18421 aatcatgcgg aaaatatcga cgaagcatgg agaattatga ccgcgcagat tctggctgca
    18481 aaaagcgagg tacagcctca tgcaaaaaca gcataacgtc gtagcgggcg tggatatggg
    18541 ggcaacgcat atccgctttt gtctgcggac agcagaaggt gaaacgctac actgcgaaaa
    18601 aaagcggacc gcagaagtta ttgctcccga cctggtgtcg ggtatcggcg aaatgattga
    18661 cgagcaactc aggcgcttta acgctcgctg tcatggtctg gtgatgggat ttccggcgct
    18721 ggtcagtaaa gataaacgca ccattatttc tacgcctaac ctgccgttaa cagcggcaga
    18781 tttatatgat ctcgccgata agctcgaaaa tacgctgaat tgtccggttg agttttcccg
    18841 cgacgttaac ctgcaacttt cctgggacgt agtagaaaac cgccttacgc aacaactggt
    18901 tctggcggcc tatctcggta cggggatggg gttcgcagtg tggatgaacg gtgcgccgtg
    18961 gacgggtgca cacggtgtgg caggcgagct gggccatatc cccctgggag atatgaccca
    19021 acactgcgcg tgtggcaatt ctgggtgcct ggaaaccaat tgctctggaa tggcgctaag
    19081 acgctggtac gaacaacagc cccgaaatta cccattgagc gatcttttcg tccatgcgga
    19141 aaacgcccct ttcgtccaga gtctgcttga aaacgcggca cgagccattg ccaccagcat
    19201 taatctgttc gatcccgatg cggtgattct gggcggtggc gtgatggata tgcccgcctt
    19261 cccacgcgag actctcattg ccatgaccca aaagtacctg cgccgtccac tgccgtatca
    19321 ggtcgtgcgc tttattgccg cctcatcttc tgactttaat ggcgctcagg gtgcagcaat
    19381 attggcgcat caacgttttt tgccacagtc ctgtgctaaa gtcccatgag taaagcgttg
    19441 gcacagtagc gcaatgtccc gctgtgccaa cgacaggtac aatatatata ataattacat
    19501 aaaacagccc atattattcc cagcaatgat agttctactg taattttccc tcttcagcac
    19561 aaatgcctaa taaaatggct cgttccgctc acacttccac ttattaaagt cgcaaattcc
    19621 caattatgat aacgccagct ttaaatgacg tattttgcat ggattacata tttacgacat
    19681 aaatgataaa tggagattta tatgaataac tctcggttat tccgtttgag caggattgtt
    19741 attgcgttca ctgccgccag cggcatgatg ataaataccg cttacgcaac agatgaggcg
    19801 aaagccgcca ctcaatatac ccaacaggtt aatcagaatt acgccaaatc attaccgttt
    19861 agcgatcgcc aggattttga cgatgctcag cgtggattta tcgctccgct gctggatgaa
    19921 ggtattctgc gtgatgcgaa cggtaaaatt tactaccgcg cgaatgatta caaatttgat
    19981 attaatgccg ctgcgccgga aaccgtaaac cccagcctgt ggcgtcagtc gcagatcaac
    20041 ggcatttcag gcctgttcaa agtcaccgat aaaatgtatc aggtgcgcgg ccaggatatc
    20101 tctaacatta cgttcgttga gggcgagaaa ggcattattg ttattgaccc actggtgacg
    20161 ccacctgccg caaaagccgc acttgacctt tacttccagc atcgtccgca aaaaccgatt
    20221 gttgccgtta tctacactca cagccacgcc gaccactacg gcggcgtgaa aggtattatc
    20281 tctgaagccg atgttaaatc cggcaaagtt caggtgattg ctccagctgg ctttatggac
    20341 gaagccatca gcgaaaacgt gctggcgggt aacatcatga gccgccgtgc gctctactct
    20401 tacggtctgt tactgccgca caacgcgcaa ggcaacgtgg gcaacggcct cggcgtgacg
    20461 ctggcaacgg gcgacccgag cattattgca ccgactaaaa ctatcgtcag aactggcgag
    20521 aagatgatta tcgacggcct ggagtttgac ttcctgatga ccccaggtag cgaagcgcca
    20581 gcggaaatgc acttctatat tccggccctg aaagccctgt gtaccgccga gaatgccacg
    20641 cataccctgc acaacttcta caccctgcgc ggcgcgaaaa cccgcgacac cagcaagtgg
    20701 accgagtatc tgaacgaaac attggatatg tggggtaacg acgcggaagt actgtttatg
    20761 ccgcacacct ggccggtctg gggcaataag catatcaatg attatattgg taaatatcgc
    20821 gatactatca agtacattca cgaccagacc ctgcacctgg cgaaccaggg ctacaccatg
    20881 aatgaaatcg gcgacatgat taagctgccg cctgcgcttg ccaataactg ggccagccgc
    20941 ggctattacg gttctgtcag ccacaacgcc cgcgcggtat ataacttcta tcttggctat
    21001 tacgacggta acccggctaa cctgcatccg tatggtcagg tggagatggg taaacgttac
    21061 gtgcaggcgc tgggcggttc tgcccgcgtc atcaacctgg cgcaagaagc gaacaagcaa
    21121 ggtgattatc gctggtcggc agaactgctg aaacaggtga ttgccgccaa cccgggtgac
    21181 caggtcgcga agaatctgca agcgaataac tttgaacagc tgggctatca ggccgagtcc
    21241 gccacatggc gcggtttcta cctgaccggc gcgaaagagc tgcgcgaagg ggtgcataag
    21301 ttcagccacg gcaccaccgg ttccccggac accattcgtg ggatgtcggt cgaaatgctg
    21361 ttcgacttta tgtccgttcg cctcgatagc gcgaaagccg cgggtaaaaa tatcagcctg
    21421 aacttcaata tgaacaacgg cgataacctc aacctgacgc tgaacgatag cgtgcttaac
    21481 taccgtaaaa cactgcaacc gcaagctgac gcctctttct acatcagccg tgaagatctg
    21541 cacgccgtgc tgaccggaca ggcaaaaatg gctgatctgg tgaaagcgaa gaaagccaaa
    21601 attattggca atggtgcgaa actggaagaa attatcgcct gtctggataa tttcgatttg
    21661 tgggtgaata tcgtaacccc aaattaatat cactcgcttt tg
//
LOCUS       DAJOTB010000077        21355 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJOTB010000077 DAJOTB010000000
VERSION     DAJOTB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.077"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..2022)
                     /locus_tag="OHN28_002824"
                     /pseudo
     CDS             complement(1..2022)
                     /locus_tag="OHN28_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612776.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2019..6734)
                     /locus_tag="OHN28_002825"
     CDS             complement(2019..6734)
                     /locus_tag="OHN28_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298194.1"
                     /translation="MRKSWTIKEDCKLLTLVRQHFSALVSHNRLNATMPFSQQLHDAF
                     DSPDRDAAALLYRLEQAKILGFASRPGGDPTKQLFRCLINNDLALYDYSLTFPSLRKA
                     LHPDTVAAALNHFTNSNPHEPLSNTINEIAKALHLSSMQIEKLLIDSGQITINSYREC
                     ERVGEITINNNLQDLISRQIPDITLVEDINACRAQVSQLYHVHECDGAEVIFSSDGTG
                     FGKSYGVIQGYVEYLERFAKNRKSDDLFPEGGFTNLLFMSPQKSQIDLDSSQKDKILA
                     AGGEFICVLSRKDIADLDFMDWASGLKNRDRYIQWYEGAKGSKYIGGAMRSLNYHVSQ
                     IDRCEEQLKKLTTYGSQDTNYEREIIEEQLKNCRHSIRNTIESACKLLFGPDSEKTSI
                     KEYIRRGLQARQERMQNAETARKPGKPEPKISVHEVYFELIKQVLPFEVCQYRPSVLL
                     MTTNKFDTSTYRLVPRQRGEGVRFESVGFDLLVGGKLTPKDPQISAVAAAGHTGQVAY
                     LRDEHFRHNPDCPFRKKNIRFTVIIDELHEAYTRLEETCHVKLITQENNLAHVISVAG
                     RIYNAVLSLERRNKPKESQTTFEQEMVKFITTLRHLLAEKCELSPGTTLGSILEMFRD
                     QLGAFEVNGDAAERIISITRNVFSFNPKMYVNEEGLKRIRMRNSEGDITRTELYYEVE
                     NDASDTNPTLHDLFQLVSVILAACSDITNRHFKRWVKNGGQDNSSSQNTPLGQFVDAA
                     NNVAGVVRHIFDRTTDENLLIDHFYTYLQPKTVFTMTPIAELNYVNSGAERTIILAFE
                     MDLIQELPEAMLLRLLTSTHNKVIGLSATSGFSHTKNGNFNRQFLARYSRDLGYRVVE
                     RKKTDIDTLRALRKLRASIRNVDFRVFDDKQLKLTDIYQNCELYRRTYDNFFDALKKP
                     LEYDLKNTYKRRQYQRELEALLLAAWEGKNSLILSLSGTFKRAFISAWRTHQSAWRQQ
                     YGMRSRCDEKTDNDKKHDQILTFTPFKGRHTVHLVFFDSPLANVEDIRQETYLQDSNT
                     VLVFMSSYKSAGTGLNYFVKYHDGDLNDINAPRLDVDFERLVLINSSFYSEVKDNSGN
                     LNTLPNYVTVLKHYADDDITVHKLADFNVNFAHGENYRLLIAEHDMSLFKVVVQAVGR
                     VERRDTLLKTEIFLPRDVFRNVAFQFAALSEDSGNEVVSESMSLLNHRLMEECEKLSQ
                     NQSFSDAEQRYAFEQAIVENGRRIDAVHKRVLKTDWINQVRAGNLEYLELCNLFRDSD
                     SFTDPMRWLEKLQANSLYAANRQMQSIHHALFIDRHQGNQTILLCHKRGPDGLVHRDY
                     AALSDFAGGAREYRPELTLFPQYRNDVDFTPGNLVGELIRECDNIQETAFKKWVPNPR
                     LVPLLKGNVGEYLFDKVLKSYGVTPLSDQRVFERLEPLVYEFFDRFIEVGNDLLCIDV
                     KRWATQLDDLTRAEETLEKSNNKIREIRNITSQKADTEGQKQIQTALAGHYERIRFIY
                     LNVAYSQNPNNLMWQDNVDHTIHYLNLLQTDYQYYQPKNRESGRAQENSKLSMTLDIN
                     PMLLTLLGVEKLPTKGKVS"
     gene            complement(7720..7891)
                     /locus_tag="OHN28_002826"
                     /pseudo
     CDS             complement(7720..7891)
                     /locus_tag="OHN28_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585271.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(8302..9099)
                     /locus_tag="OHN28_002827"
     CDS             complement(8302..9099)
                     /locus_tag="OHN28_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2298195.1"
                     /translation="MLQNNIKQLRTQLSITQRELAFMVGTSQQQIQRIETGKVAAKLG
                     LAQAICNALDKPLNVVFPESDRLINNFRKKRRKTDEDLEAIATSGIEMDSGLWTVKLW
                     LQGQRDYLLLPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVVFHQFL
                     SDGLRPIVDEEDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFD
                     ALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
     gene            complement(9213..9488)
                     /locus_tag="OHN28_002828"
     CDS             complement(9213..9488)
                     /locus_tag="OHN28_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2298196.1"
                     /translation="MEIYWTLKAQDDLERIYRFALQYSRQHADDVLDRLITGCAGLTA
                     HPAIGVQQTRYEPREVRKVLFDDYEVHYELCDNDIYIVDLWHTREER"
     gene            complement(9492..9740)
                     /locus_tag="OHN28_002829"
     CDS             complement(9492..9740)
                     /locus_tag="OHN28_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCQ2298197.1"
                     /translation="MGSLTKQTISAQIPIELAAAVENLAIELDRSKSWIIKEALTSML
                     AERERRHQSIQAGLADVDAGRVMSHSDMVDFANRLKKS"
     gene            complement(9817..10650)
                     /locus_tag="OHN28_002830"
     CDS             complement(9817..10650)
                     /locus_tag="OHN28_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2298198.1"
                     /translation="MQTEAEVLTDHNELICSTSIERIVTGRDAALSLIEILIHKLDDI
                     SRLTSSIGGDVAEHWAMRQGHSFDCWLVQPVDKAMPVITRNIDRSIWRDLMLKSGMLT
                     LMDAEARSQWAKNLEEGDLPAISEANILSTFEQLHHNKQDVFERGIINVFKGLSWDYK
                     TNNPCYFGKKIIVNNLVKHDRWGYSLNWCWRRDQLADLERMFYLLDGKAIPDNRHDIS
                     IRFMDFVRDNPHQQIFEDELFTIRYFQKGSGHITFKRLDLVDKMNDIVAKHFPGMLPA
                     K"
     gene            complement(10781..11116)
                     /locus_tag="OHN28_002831"
     CDS             complement(10781..11116)
                     /locus_tag="OHN28_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2298199.1"
                     /translation="MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFN
                     NEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGFSWQEQTPYLRAVDILRARQATG
                     LLRRRHNTP"
     gene            complement(11147..11482)
                     /locus_tag="OHN28_002832"
     CDS             complement(11147..11482)
                     /locus_tag="OHN28_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2298200.1"
                     /translation="MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITG
                     KFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQRTVTLYAKGLTCEADTLGSCGYV
                     YIAIYPTQR"
     gene            complement(11482..11955)
                     /gene="radC"
                     /locus_tag="OHN28_002833"
     CDS             complement(11482..11955)
                     /gene="radC"
                     /locus_tag="OHN28_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCQ2298201.1"
                     /translation="MSELMLTPVFSVSEQRVIRRAMRLLEKYQRNPGESFLSSDATKT
                     WLQLHLARQEREMFVVLYLDNQNRLLEHETLFLGTINSTEVHPREIVKSALRHNAAAV
                     ILAHNHPSGVAEPSRADRLITTNIVKTLDLVKVRVLDHFIIGAGDILSFAERGWL"
     gene            complement(11985..12803)
                     /locus_tag="OHN28_002834"
     CDS             complement(11985..12803)
                     /locus_tag="OHN28_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2298202.1"
                     /translation="MKLASRFGRVNQIRRDRPLTHDELMQYVPSVFGENKHESRSERY
                     TYIPTITLLDNLQREGFHPFFACQTRVRDVGKREHTKHMLRLRREDQINRNQVPEIIL
                     LNSHDGSSSYQMLPGLFRSVCCNGLVCGESFGEVRVPHKGNVVEKVIEGAYEVLSVFD
                     QVEEKRDAMQSLMLPPPAQQALAKAALTYRFGEEHQPVTESQLLAPRRWQDESGDLWT
                     TYQRIQENLIKGGLNGRTAKGGRTHTRAIKGIDGDVRLNRALWVMAENMLQLAS"
     gene            complement(13039..13992)
                     /locus_tag="OHN28_002835"
     CDS             complement(13039..13992)
                     /locus_tag="OHN28_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298203.1"
                     /translation="MAVNVRSSIAVKDIRTSRFLFWYIRKNIQGANQFAFDGNNPDTS
                     LSERIINTALEAGYPDPIQRSNFIRSLELSINTTLLPEKYFSWLKENERATFWLWGVM
                     CLDYRCMESINEKLGTNLNTNWYQKAGITDSPASHNERYSIIVALLDYINIETNQAPL
                     MRKWLYERLVFWRKNEHQRIKLRWLNEDNDEVCIWAYNYINKFQKEHGGNTGNHLDPV
                     QIPEPLNCVETYHAVYAMLDLWSADDDLQQKAVKKINKAFYQKNFRRKLSEKRERESI
                     SDTHKERLDFLVKFYKSDKISVIERLIDERVQGIETRLSRG"
     gene            14585..15199
                     /locus_tag="OHN28_002836"
     CDS             14585..15199
                     /locus_tag="OHN28_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2298204.1"
                     /translation="MKLNERYGKPNAHYMKRLEEITRYAMDDYARILAFRVDLLLPVD
                     NSHKGYDSAVITRFIASLKAQIAAYLQRRRRDGKRVYPCKLRYAWVREFGELNGNKHY
                     HMLLLVNREVFRNTMLNTENSAQPRGLLAGMVARAWAVALRRTGDMANKAPFHLPGVS
                     YELDRRNGTNTDFFRSYIYHISYLAKEYTKKHDDGERNFGCSQG"
     gene            15317..15535
                     /locus_tag="OHN28_002837"
     CDS             15317..15535
                     /locus_tag="OHN28_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCQ2298205.1"
                     /translation="MNNQPNTDTDLMNDKLVTMAFITTITGLTDKWFYKLISEGKFPK
                     PIKLGRSSRWRESEVKRWLTERIAESRY"
     gene            complement(15756..16541)
                     /locus_tag="OHN28_002838"
     CDS             complement(15756..16541)
                     /locus_tag="OHN28_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298206.1"
                     /translation="MKPIIIPVLFVSLFAFHPAYAVGAYDLQCLLDNGEQMTLSHVSN
                     TVYISFETPGGDSEEGGSVIKLDIPSGEAKQTLAANPGAGIASFTLRGENEDIEGAVA
                     VNYSEYDGTGDAYYTAMNAMGQETSTVSCKPGTIKVSRSLLQNGINGVGSQQANKPAP
                     SQQQQAQQSTTPPFKVQFGSSVSNEGWNTRYGVIQLTITDDNVVLKSIRVNRGNCKME
                     SVGNRTLPAKYKFGDVATFKYMKCDRIIEADIVTDTGSWTFNS"
     gene            complement(16554..17567)
                     /locus_tag="OHN28_002839"
     CDS             complement(16554..17567)
                     /locus_tag="OHN28_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2298207.1"
                     /translation="MDDPVKGEGRMITDASGVTRREGGIFGMWGNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPGVGENARRDKEYEDLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QRINIEKKEAYITERFGFTHYPVTPVSADEGYNILRLVETIIRALPDRAKSSTASQVK
                     DEFKTDEVKTEAKNGFGSVISGILDDVIDSFPLPKPVKELTRKGKDKLIEWAGNAWDH
                     FFG"
     gene            complement(17607..18290)
                     /locus_tag="OHN28_002840"
     CDS             complement(17607..18290)
                     /locus_tag="OHN28_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2298208.1"
                     /translation="MKTLHDHYDRLSVRLSVIITRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTRRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            complement(18606..19583)
                     /locus_tag="OHN28_002841"
     CDS             complement(18606..19583)
                     /locus_tag="OHN28_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HCQ2298209.1"
                     /translation="MTSLVFSYSHADEALRNELEKHLSPLKRTGKITTWHDRRIIPGQ
                     EFENQIDHYFSQADIILLLISSDFIASDYCYQVEMTNALERHNRGEAVVIPVILRECA
                     WHQLPFGSIMAATIDGKPITRFASHDEGYVQVVDAVSRAIANLEAKKPQQIVHTPVPA
                     NPMFQATDTVFTPRSSNLSLPKHFTDLDKDRACRDGFEYVARYFEGSLTELKKRHAGL
                     EVDFNKRDANAFTCAIYIGGSKVGQCAIWLNSGRNMGIGDIGYSQDGVIGNAYNEILT
                     IDNNGQILGFRPSMGVFSPGYSRDTLLTNEGMADHLWDMFFEPIKQQAR"
     gene            19797..>21355
                     /locus_tag="OHN28_002842"
     CDS             19797..>21355
                     /locus_tag="OHN28_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012666947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCQ2298210.1"
                     /translation="MDNAEQQFLNDLDTKFWKAADKLRANMDAANYKHVVLGLIFLKY
                     VSDAFDARQKELKGLFEDKFNPDNIYALSRDDYDTEEAYQQAIAEELEVQDYYTEKNV
                     FWVPKLARWETLKTNAALPVGTVLGKDDSGKDIVMTSVSKLIDIALDTIENSNPKLKN
                     ILNRIGHYQLGNDLLISLINVFSDTSFSEPKYNGVKLNLKSKDILGHVYEYFLGQFAL
                     AEGKQGGQYYTPKSIVTLIVEMLQPYNGRVYDPAMGSGGFFVSSDRFIEAHADEKHYN
                     AAEQKRNISVYGQESNPTTWRLAAMNMVIRGIDFNFGKQNADTFLNDQHPDLRADFVM
                     ANPPFNMKEWWNAKLEDDVRWQYGTPPQGNANFAWMQHMIHHLAPKGSMALLLANGSM
                     SSNSNTEGDIRRQIIEADLVECMVALPGQLFTNTQIPACIWLLTKDKSGGNGKAHRKG
                     EVLFIDARQIGFMKDRVLRDFSTDDIKKIANTFHAWQTDKDYEDEAGFCFAATLDDIK
                     KNDFVLTPGRYV"
BASE COUNT         5610 a         4968 c         4919 g         5858 t
ORIGIN      
        1 cgcttaccag aacaaacact ccattctcat gcggttgcag gtaaatatgg tgatgcttca
       61 tcttttgagt tttggtcaac gagtcgtatt tcgagcggcc cggtgatatc agatagggca
      121 gtacgttttg ctccaatttt gctgagcggg taatggtata cccgttaaat ttgtcacctt
      181 gacgctccgc tagcgctttt ttttgttcct gataatcgta tgaatcgacg atattaaatt
      241 gcgccgggcc aatcaggcgc ggtacgcggg agctgttata ggtggtaagc agcacatctg
      301 ctgtatggtc gtagagatag aagcttttat cgaataattt ctttagtcgt cccagagctg
      361 gatgatcaac taatagatac tcgaattcac cttcgacgac cccggtatca acccccctaa
      421 gttggccgtg atctattttt agttcaacat acccaagcag atggatatta cttttcgttt
      481 tgcgtaccgc ataagcggta tagtgcccgt tcgccaattc tggcagtggt atgttatgtt
      541 gattgagtaa gctctctttg aaccatagct cggtttttgt tcgacgtacc ttacttttca
      601 ataaggtaac tgctgacttg agacgactgt gtggtttcgt aagttcgatt tcgtattgcg
      661 cagggttgct taccaattgt tcattaataa aatcaatcgc atccagcaac ttacgatcaa
      721 tgttcattcc ttgtaaaacc acaggcagcg gttgctgatt tagccaccct tgcaaacgat
      781 ctagcttaag cttggtataa atatcccagt tgagttcagg ttgttttcgg acgtcggcaa
      841 aggctgcata gaaatcattg tactcaacag attcattctc ttgctgccgg atgctgttgc
      901 ttcgttcttc atctaccgca ttgagtacaa gaatagatgt attgacaggc agcccggcaa
      961 atctggtttg ctgagaatgt ttaagcgatg ccagcggcca gagtttgtac ccggggaact
     1021 ggttggctaa tgcgtcaaag aaataagctt cttgcgtggc gctgaattcg acgccgttat
     1081 taaccaccaa cagcgtatta gccagctgtt gatcgaggtc ggtagcaaac tgatcgacct
     1141 cgtgtgtagc ctgaaatggg ataggttgat gggggatctc tagttcactg agccgctcgc
     1201 aggcagcatt catgaccacg ttataggtat aggcctggca ttcgccatag ccgctgcgct
     1261 cacgaaagaa ttccagtttg ctatcccggg catcgagttt gcgccgggct ttaagccgac
     1321 gattatcgat gttaaaccac atgctgatat cgtccagaga tagatgcatc tcatccgcca
     1381 gttcgaccag gaattttcgt ttgtgcaggg taaaggcgag ctcgttatat tctgatgcat
     1441 aaatgtcgag ctcaaaggct tccatcaccc cggtgctttt ttttgcgtga cgggctgact
     1501 caggcgagcg ttaaggtaaa gcgttgagtt gaagtaagtc ggcgataccg aaaagcgctc
     1561 actggctttt tccagcatta ccattttcaa atgcagacta aataatccct gaatcaagcg
     1621 agataggtca taattttcag cagtgagttg ctgacaaagc gtttcaatac gttctgccaa
     1681 cgtcctgtga accgggctgt tcggcgcagt tgcaggcgcc aggaaaaaaa agacgtgatg
     1741 gtaatttggt aagcaatgca gttcatattt tacctcctgc ccggcctcca gcccaagctt
     1801 ctgccgcacg caagatttta cctcctgatg aattcgcgca tagtaatcag cggatttttc
     1861 ttttcgctct ttagtaaagc gctgaaccat aaagtctatc gtatagatat tagatacagg
     1921 gaccaataga cgctggatgg tcgtcagctg atcttcttca agcttgatca aattggttcg
     1981 ggcaatcgga gtatccgtca gttcattcaa attagggatc atgagacttt tcctttagtc
     2041 ggcaactttt ctacacccag cagggttagt aacattgggt taatgtccaa tgtcatgctc
     2101 agtttcgagt tttcctgagc tcgcccgctc tctctatttt tgggctgata gtactggtag
     2161 tcggtttgca acaggttaag gtagtggatc gtgtgatcca cgttatcttg ccacatcaga
     2221 ttattggggt tctggctgta ggcgacgttc agatagataa atcgaatacg ctcataatgg
     2281 cctgccagtg ccgtctggat ctgtttctgc ccctctgtat ccgccttttg gctggtgata
     2341 ttacggatct cgcgaatctt gttgttgctt ttctcaagcg tttcttctgc ccgcgttaaa
     2401 tcatccaact gcgtcgccca gcgtttaacg tcgatgcaga gcaggtcgtt acccacttca
     2461 ataaagcggt caaaaaactc atatactagc ggttcaaggc gttcaaacac ccgttgatca
     2521 gaaagtgggg taacgccata gctttttagc actttatcga agagatattc accgacattg
     2581 cctttgagca acggaactag cctggggttg ggtacccatt ttttgaatgc cgtttcctgg
     2641 atgttgtcac attcacgaat caactcaccg accaggttgc cgggggtaaa atcgacatcg
     2701 tttctatact gcggaaagag ggtgagctca ggccggtact ctcttgcgcc gccagcgaaa
     2761 tccgacaggg cggcataatc tctgtggaca agtccatccg ggccgcgttt gtggcaaagt
     2821 aaaatagttt ggttgccctg atggcggtcg ataaacaggg cgtggtggat tgattgcatt
     2881 tgccgattgg cggcatacaa ggaattagcc tgaagttttt ccagccaacg catgggatcg
     2941 gtaaaggagt cagaatcgcg gaataaatta cacaattcga gatattcaag gttgccggcg
     3001 cgtacctgat ttatccagtc ggttttcagg actcgcttat gaacagcatc gatgcggcga
     3061 ccattttcta cgatggcctg ttcaaacgca taccgctgtt ctgcatcact aaatgactgg
     3121 ttctgactca gcttttcgca ctcctccatt aggcggtggt taagcaagga catgctttct
     3181 gatactacct cgttaccgct atcttcactg agagcggcga actggaatgc aacattacgg
     3241 aacacatcgc ggggtaaaaa gatttctgtt ttcaatagag tgtcacgacg ctcgactcgc
     3301 cctacggcct gcacgacgac tttgaataag ctcatatcat gttcggctat taacaggcga
     3361 tagttttcgc cgtgggcgaa attgacgttg aaatcggcca gcttgtggac ggtaatatcg
     3421 tcatcggcgt agtgtttaag cacggtaacg tagttaggta atgtattgag gttgccgctg
     3481 ttgtccttta cttcgctgta aaacgaggag ttgatgagta ctaagcgctc aaaatcgaca
     3541 tctagacgtg gtgcattgat atcattaaga tcgccgtcat ggtatttaac aaagtagttg
     3601 aggccggtgc ctgcactttt gtagctgctc ataaatacca gtacggtatt gctgtcctgg
     3661 agataggttt cttgcctgat atcttcgaca ttggccagtg gtgaatcgaa aaagaccaaa
     3721 tggactgtgt gacgcccttt gaatggggta aaggtcagga tctggtcatg tttcttatca
     3781 ttatccgttt tttcatcgca ccgggagcgc ataccgtact gctgacgcca ggctgattga
     3841 tgagtgcgcc aggcgctgat aaaggcccgc ttaaatgtcc ctgaaagtga caaaatcagg
     3901 ctgtttttac cctcccaggc ggcaagcagt aacgcttcca gttcccgctg gtactgacgc
     3961 cgtttatagg tatttttcag gtcgtattcc agcggtttct tcagcgcgtc gaaaaagttg
     4021 tcatacgtcc tgcgatagag ctcacaattt tggtagatat cggtcaattt taactgctta
     4081 tcatcgaaca ccctgaagtc gacgttgcgg atactggctc tcaacttgcg tagtgctcta
     4141 agcgtatcga tatctgtttt tttgcgttca acgactcgat agccaagatc acgactataa
     4201 cgcgccaaga attgacgatt gaagttaccg tttttggtgt ggctaaaacc gctggtggcg
     4261 ctaagcccaa ttactttatt gtgcgtgctg gttaataaac gcagcagcat ggcttcgggc
     4321 aactcttgta tcagatccat ctcgaacgcc aggataattg tgcgctcggc tccgctgttc
     4381 acgtaattga gttcagctat cggcgtcatc gtgaatacgg ttttgggttg caggtaagta
     4441 tagaaatgat caatcaacaa gttttcatcg gtggtgcgat cgaagatgtg gcgtaccacg
     4501 ccagctacgt tattggctgc gtcaacaaac tggcccaaag gggtattctg gctgctggag
     4561 ttatcctggc cgccattctt tacccagcgc ttaaagtgtc ggttggtgat atcagaacag
     4621 gcggcgagga tgacggagac caactggaac agatcgtgca gggtgggatt ggtgtcgctg
     4681 gcatcatttt cgacttcgta atacagctcg gtgcgcgtta tgtcgccttc gctgttgcgc
     4741 atacgaatgc gtttcagccc ctcttcattg acgtacattt tggggttaaa gctgaacacg
     4801 ttccgggtga ttgagatgat gcgttcagcg gcgtcgccgt tgacttcaaa tgcccctaac
     4861 tggtcgcgaa acatctccag tatcgatccc agcgttgtgc cgggtgatag ttcgcacttt
     4921 tctgccagta aatggcgcag agtcgtgatg aatttgacca tctcttgttc aaaggtcgtt
     4981 tgcgattctt tgggcttgtt ccggcgctca aggctgagta ccgcgttgta aatacgtccc
     5041 gcgacagaga taacgtgcgc caggttgttt tcctgtgtga tcagctttac atggcatgtt
     5101 tcttcaaggc gagtgtaggc ttcatgtagt tcatcaatga tcaccgtaaa gcgaatattt
     5161 ttcttgcgaa aaggacaatc tggattgtgt ctgaagtgtt cgtcacgaag ataggcaacc
     5221 tgcccggtat gaccggctgc cgcaacggcg ctaatctgtg gatctttggg agtcagctta
     5281 ccaccaacca gcaagtcgaa acctacggac tcaaaacgca cgccttcgcc tcgctggcga
     5341 ggcaccagtc ggtaagttga tgtgtcgaac ttattcgtgg tcattaatag caccgacggg
     5401 cggtactggc aaacttcgaa aggcaatacc tgtttgataa gctcgaaata gacttcgtgt
     5461 acgcttatct taggttcagg cttccctggt tttcgtgctg tctccgcgtt ttgcattcgc
     5521 tcttggcgcg cctggagccc gcgacgaatg tactctttaa tggaagtttt ttcactatct
     5581 ggtccaaata gtaatttaca ggctgactca atcgtattgc ggatactgtg acgacagttt
     5641 tttagctgct cttcgataat ttctctttcg tagttggtat cctgagaacc gtatgttgtc
     5701 agctttttta actgctcttc acagcgatca atttgtgaaa catgataatt gagcgaacgc
     5761 atagcgccgc cgatatattt gctacctttt gcaccttcgt accactgaat atagcggtcg
     5821 cggtttttca ggccagaggc ccagtccata aagtcgagat cggcaatatc cttacgagag
     5881 agaacgcaaa tgaattcgcc gccagcagcc agaattttgt ctttctggct gctgtccagg
     5941 tcgatttgtg atttttgcgg cgacatgaac agcaggttgg taaagccacc ttcgggaaac
     6001 agatcatctg actttcggtt tttggcgaaa cgctccagat attcgacata cccttgaatt
     6061 acgccatagc ttttgccgaa tcccgtgcca tcggaactga agatgacctc agcaccatca
     6121 cattcatgca cgtggtaaag ttgagagact tgggcacggc aagcgttaat atcttcaacc
     6181 agcgttatgt caggaatttg cctggagatg agatcttgca gattattatt tatagttatc
     6241 tctccaacac gctcacactc acggtaacta ttgatggtta tttggccact atcgatcaga
     6301 agcttttcta tctgcatgga ggaaagatgc aaggcttttg caatttcatt gatagtattg
     6361 gacagtggtt cgtgtggatt gctattcgtg aagtggttta gtgctgctgc aacggtatct
     6421 ggatgcaatg cttttctgag agagggaaag gtgaggctgt aatcgtatag cgccaaatca
     6481 ttgtttatca gacagcgaaa cagctgttta gtgggatcgc ctccaggacg gctggcaaat
     6541 cccaggattt ttgcctgttc gagtcgatac aacaatgctg cggcatcgcg atcaggtgag
     6601 tcaaatgcat cgtgaagctg ctggctaaat ggcattgtgg cattcagccg gttatggctg
     6661 accaacgcgg aaaagtgctg acgtaccaag gtaagcagct tacaatcttc ctttatagtc
     6721 cagctttttc tcattcttct tccccttaat ataaatgcac ctgcaaccac tcagccaaac
     6781 gctcaacgtc aaaaaaccgt aatccatact cccacgccaa aaacgtcttc gataaatatt
     6841 catcatattt tcgaggcact tttcagcgat gctcttggca agacgggact cagggggtaa
     6901 gcatctgctt aatttcccga gcgtgttttt tcaatttcaa ctacggcaca acaatatgaa
     6961 cgcacatctc ttcatcgcct gatcaaagta gctgccaact atttttcgcc tcaacattga
     7021 tgttttttta ctaacgattt cgactacctt ccgaagcgac cacaacaaat aaaatattgg
     7081 ttacatatca gtcttatatc accggagcaa gttgactttt gataagctat ttcaagatat
     7141 taatagctga tcatgcttcc ccaaattgct tacttatagc aaagtcatat agcataaact
     7201 gaagggcgaa ttgttaaatc ataccgtgac tgagctgaag aacaagcata aaaattatat
     7261 catttacata gcttatgtat tttagttaaa taataaatag gcagtataca atccaattca
     7321 acagccgagt tcaagcgatt aattttttaa tgagcttatc aagcacgagc agcgatacca
     7381 gcaaatccac ttagttgctg gaagattatg agtccttcaa cacatagcag attatctccg
     7441 ccgttggtag agaaccgttc accgttgtta gcccacagtt ataaatttat attaatgact
     7501 tgaagaatct atcttgggta gagcatcaac attacttatt ctaaaccacg ttacacatga
     7561 tctccggggc agcgaagtca tcaaaaaaga gtattataaa actccgccct taaggagaaa
     7621 ggtttcctgg cagttgtaat gccgttgacg atacagcgca caacacggat ataaacagcc
     7681 atttgattga taaatatctg aagggctgtt tatattcaat caagctaacg attcattaat
     7741 ctttacatag agatgaggta atgtggcctc attgcgattc gcatccagat aatccgcccc
     7801 cactgcagca tctgccgctc taccgcatct ctcggccata gccccgactc aatcaacgac
     7861 tacacgccat tgccctgaaa ccatgcccac aaaaagcagc tctcaatttc cattcatgct
     7921 ttataccttt tctaataggc ttttgcttca ggcttagccg aacgaacctt cacatcactc
     7981 aaagccatga tgaataccct ttcaagggtt ttgtgtggct acaaacatta tcgaatcggg
     8041 atatacccgc agctgtagct gtacctgcat cagtaagttg atgtaggttg gcttatgttg
     8101 agcgaaaaat agcggaaacc ctgtgtgaca ctgatttcag gcacaaaaaa agacgtctat
     8161 tgatgttccg atggtgcgaa ggccggaccg tgattccaac ctcaacaata tgaaattaat
     8221 ggatttttaa aaatgcgaag ttactttgta tactcacatg tatactcagt actatttctt
     8281 aagaaaccaa ttcattcaat actactcatc atcgtcctcc actggttcca gcacatcaag
     8341 cgcgactcgc accatagcga tagaaccaat acgaatgaaa gcatcttctc catcttcgtc
     8401 ggttatcata aaacgatccg tggtttcagg ctcgcaatcc agcgcgtcaa aaaaagcatt
     8461 caactggccg atatcatccg tctcttcgtt ttgcggcgca tctggctcaa cactcagtgg
     8521 aatcactggt ccgccgttta ccagcgtgat atggacatta aaataatcgt cttcgtaggc
     8581 atcatcctct tcgtcaacga ttggccgcag accatcagaa aggaactgat gaaataccac
     8641 ttcacgcata tttaacgcat aacgatactc ggaagaatca aaaacgaaga agcgttctat
     8701 gtttggagtg tttttttcct gaaaataata ataaaacctc cgcttatccg ctgctgaaat
     8761 gggcaacagc aaatagtccc gctgcccctg tagccaaagt tttacggtcc agagaccgct
     8821 atccatttca atgccgcttg tggctatagc ctcaagatct tcatcagttt tacgacgctt
     8881 tttgcggaag ttgtttatca accgatcgct ttccgggaac actacgttta gaggcttatc
     8941 cagagcattg catattgcct gtgcgagacc cagctttgca gcaaccttcc cggtttcgat
     9001 acgctgaatt tgctgctggc tggtgccaac cataaacgcc agctcgcgct gggtgataga
     9061 tagctgggta cggagctgtt tgatgttgtt ctgtaacatg gcatcctcct gcagaaggag
     9121 tggaaagtgc cattaaaaca caaaattgag tataaacaca taatcgtgtt tatactcact
     9181 aattaacacc cttgaacgaa aagaaaatct cttcatcgtt cttctctggt atgccagaga
     9241 tccacgatat aaatgtcatt gtcacaaagt tcgtagtgaa cctcatagtc atcaaataac
     9301 acttttcgga cttcccgagg ttcataccga gtctgctgta caccaattgc cggatgtgct
     9361 gtaagaccag cacaaccagt aatcaggcga tccaaaacgt catcggcgtg ctggcggcta
     9421 tactgaaggg caaaacggta gatacgctca aggtcatcct gagctttaag cgtccagtaa
     9481 atttccattt atcaggattt ctttaagcga ttagcaaaat ccaccatgtc cgagtgactc
     9541 atcacgcgac cggcatccac atccgcaaga ccggcctgga tgctctggtg acgccgttct
     9601 ctttcggcca gcatactggt caacgcttcc ttaataatcc agcttttaga acgatctaac
     9661 tcaattgcca gattctcaac agcagcggcc aattcaatag gtatctgtgc gctgatggtt
     9721 tgtttagtaa gcgaacccat agggattacc tcgtttaata acatttaata actcctatta
     9781 atattagcat agaggtgcct ataaaaacat cacatctcat ttcgcaggta acatccccgg
     9841 aaagtgcttc gccacgatat cattcatctt atccaccaga tccagtcgct tgaatgtgat
     9901 atgcccactg cccttctgaa agtagcgaat agtgaacaac tcatcttcga aaatctgctg
     9961 atgcggatta tcgcgcacaa aatccataaa ccggatggag atatcatgcc ggttatcggg
    10021 aatggccttc ccatccagca ggtagaacat acgttccaga tcggcaagct gatctcgccg
    10081 ccagcaccag ttcaggctgt agccccatct gtcatgcttc accagattgt taacgataat
    10141 cttcttaccg aaataacagg gattattggt tttgtagtcc cagctcaatc ccttaaatac
    10201 attgataatc ccacgttcga aaacgtcctg cttattgtgg tgaagttgtt caaaggtact
    10261 caggatgttg gcttcgctaa tcgccgggag atcgccctcc tccaggttct ttgcccactg
    10321 actgcgagct tcggcatcca tcagagtcaa catcccggac ttcagcatga gatcgcgcca
    10381 gatgctgcga tcgatattgc gggtgatcac cggcatggct ttatccaccg gctgcactag
    10441 ccagcagtcg aaggaatggc cctgacgcat cgcccaatgc tccgccacat caccaccgat
    10501 gctggaggtt agccgtgaga tatcatcaag cttatggatc aggatttcaa tcagactcaa
    10561 cgcagcatca cgacccgtca cgatacgctc aatactggtc gagcaaatta gctcgttatg
    10621 atcggttaag acttcagctt ctgtttgcat aatcttttgt ccgtataaaa gcaaatgcgc
    10681 cagccatgaa cagcaggcgc attatgcagg gagttatgag ggaagtaatt ttggcgatgt
    10741 aaatcgggaa ggagactcgc agattcaccg tgccgtggtg ttatggcgtg ttatggcggc
    10801 ggcgcagcag acctgtagcc tgccgcgccc gtaaaatatc gaccgcacga agataaggcg
    10861 tctgttcctg ccagctaaat cctttcctgt cgatacggat caattcgtac ttctccacca
    10921 gaaaattgac ggcatcgacc agagtgatcc cggcgtctat gtgttcctga atcacctttt
    10981 cattgttgaa tggtgtgtca ttaagattca gcccgtagtg ctgctccagc aggcgggcca
    11041 gtaatatttg ccagaccacg acgggcgaca gatatgacgt agtcgcccgc agcgtcgttg
    11101 caggaataat tttcataacg atgttccgta ttaaggaggt taaaactcag cgttgagtgg
    11161 gataaatagc gatatagacg taaccacagg aacccagcgt atcggcttcg caggttagtc
    11221 ctttggcgta tagtgtgacg gtacgttgtt ggcaggcgga gagttcatcg tcagccagtt
    11281 tcagctccag ttgtttcatc agcaccggaa aagcctggtc gatatgcagc agatccgggg
    11341 agctgaattt cccggtgatt gcggcccgat cagccaggta atgcaggtta tgtctttcct
    11401 gtaccagtcg agcagtgaag cgtggtgtta cggtacaggg aagcccacat tcttgttgca
    11461 tgaccttatc cttttgcagc attacagcca gccccgttcg gcaaaagaga ggatatcccc
    11521 cgccccaata atgaaatgat ccagcaccct cactttcacc aaatccagtg tcttaacaat
    11581 attcgttgta ataagtctgt cggcccgact gggttccgcc acgccggagg gatggttatg
    11641 cgcaaggatc actgctgcgg cattgtgccg tagcgcagat ttcacaatct cacggggatg
    11701 tacttctgtg ctgttaatcg tgccgagaaa tagcgtttcg tgttccagca gtcggttctg
    11761 gttatcgagg tagagcacaa caaacatttc ccgctcctgt cgtgccagat gcagttgcag
    11821 ccaggttttt gtggcgtcag aggaaagaaa agattcgcca ggattgcgtt gatatttctc
    11881 cagcaggcgc atcgcacggc ggataacgcg ttgttcactc accgaaaata ccggtgttag
    11941 catcagttca gacatcgtgt ttcctttact attaatagcg ttgtttatga ggccagttgc
    12001 aacatgtttt cagccatcac ccacagggcg cggttgagcc tgacatcgcc gtcgatgcct
    12061 ttaatggcgc gggtgtgcgt gcgaccacct tttgccgtcc gcccgtttaa accacctttg
    12121 atcaggttct cctgaatacg ctggtaggtc gtccacagat cgccggattc atcctgccag
    12181 cggcggggag ccagcaactg ggattccgtc accggctggt gctcttcgcc aaagcggtaa
    12241 gtcagcgccg cctttgccag cgcctgttgc gcagggggtg gcagcatcag cgactgcatg
    12301 gcatcgcgtt tctcttcaac ctggtcaaaa acactgagga cttcataagc gccttcaatc
    12361 actttctcca ccacattgcc tttatgcggt acccgtactt caccaaacga ctcgccacag
    12421 accagcccgt tacaacaaac agaacggaac agccctggca gcatctgata agaactggaa
    12481 ccgtcatggc tattgagcag aataatttcc ggcacctggt tgcgattgat ctggtcttcc
    12541 cggcgcagac gcagcatgtg ttttgtatgt tcacgtttac ccacatcacg aacacgggtc
    12601 tggcaggcaa agaaaggatg aaatccttca cgctggaggt tatcgagcag ggtaatggtg
    12661 ggaatatagg tatagcgttc gctgcgtgat tcgtgcttat tttccccaaa tacgctgggg
    12721 acatactgca tcaattcgtc atgcgttaat gggcgatcgc gacggatttg atttacgcga
    12781 ccaaagcggc tggctaattt catagtaaat tccttaataa agaatataaa aaacaggccg
    12841 catcttctga aaaattgcgg ctaatttaat gcgtgattaa aacggacggt agtataggtg
    12901 ggataaaata ataggtgcga gcagatatta aattacattc cggcagtaca tatatgagcg
    12961 tgatatagtg ctgaaataat acatatctga attctcccgg tgattatcac cgggagaatt
    13021 taagtttgaa atgacacctc aaccacggga taatcttgtt tctattccct gaaccctttc
    13081 gtctattaac ctttcaataa cggatatttt atctgacttg taaaatttaa ccagaaaatc
    13141 cagacgctct ttatgcgtat cactgataga ctcccgttcc cgtttttcag aaagtttacg
    13201 tctgaagttt ttttggtaaa atgctttatt tattttcttt accgctttct gctggagatc
    13261 atcatccgct gaccataaat ccagcatggc ataaacggcg tgataggttt ctacacaatt
    13321 aagtggttcc gggatctgaa cggggtctaa atggtttcct gtattaccac cgtgctcttt
    13381 ctggaattta ttaatgtaat tataagccca tatacagact tcgtcattat cctcattaag
    13441 ccagcgtaat tttattctct gatgttcatt tttcctccag aatacaagcc tctcatatag
    13501 ccactttctc atcaacggtg cctgattagt ctcaatattg atatagtcca gtaatgcaac
    13561 gatgattgag tatctttcgt tatggcttgc aggagaatct gttattccag ctttctgata
    13621 ccagttagtg tttaaatttg tgcccagctt ttcgttaatt gactccatac agcggtaatc
    13681 aagacacatc acgccccata gccagaacgt cgctctttca ttttctttta accaactaaa
    13741 atatttctcc ggcagaagcg tcgtattaat cgacagctcc agtgagcgaa taaaattact
    13801 tcgctgaatg ggatcgggat acccggcctc gagggctgta ttaataattc tctccgaaag
    13861 ggatgtatct ggattgttcc cgtcaaacgc gaattgattt gctccctgaa tgtttttcct
    13921 gatgtaccag aagaggaacc tgctggttcg gatgtccttc actgctattg atgagcgcac
    13981 attaacggcc attgttctac ctcgtcatcc ttgttaaatc agtcgaaatc aatagcgtaa
    14041 ttatgatgtg ttttcgaagt aaaaccgtag caataaatca ataaaagtgg aaaacacgta
    14101 gcaatatcgt gttattccat agtaatagag taccattaac gtagtttttt atttatcacc
    14161 atactcacag atcccgcctg tagaaaacgg ctggatagtc ttaaattttt ccacgtatag
    14221 ccctggtggc tttacgggtg aaacctccca ggataacgag ttgacatctg gaagatgtca
    14281 ttcatgaccg caactcatag attaggtcga tgtattaatt ataacttaag agtaaaactc
    14341 tttgtcttca agaacagata attaactacg aacaccagta aataccgggc ttctactctt
    14401 tttatcaccc tttctttatg aatcaacatt gccggtatta atattatgtt tatcactcat
    14461 tcattagatt cagcatatag agggatgaca ccagagacag ggaaaagtaa tccagatcgg
    14521 catagctcag tgaaaagtta ctacagcact tatggatatg taaccaacca caaggaactg
    14581 aagcatgaaa ctgaatgaac gatatggcaa gcctaatgca cactatatga aacgtctgga
    14641 agagattact cgctatgcga tggatgatta cgccagaata ctggctttca gagtggattt
    14701 attactgcca gtggataata gccataaagg gtatgactca gcggttatta cccgttttat
    14761 agcctcactg aaagcacaga tagccgcata tttacaacgg cgcaggagag acggtaaacg
    14821 tgtttacccc tgtaagctgc gatatgcatg ggtaagagaa tttggtgaac tgaatggcaa
    14881 caaacattat cacatgctgt tgctggttaa tcgggaggta ttcaggaaca ccatgctgaa
    14941 cacggagaat tcagcgcaac ctcgcggatt gctggcggga atggtagcgc gtgcatgggc
    15001 tgtggcgctt agacgtaccg gagatatggc aaataaagcc ccctttcatt taccgggcgt
    15061 ctcttatgaa ctggaccgca gaaatggcac aaatacggac tttttccgca gctacatata
    15121 ccacatcagt tatctggcga aggagtacac caaaaaacac gatgatggtg aacgtaactt
    15181 tggttgtagt cagggataag taatttattg ccggagaagt gattgtaatg gcggagtatg
    15241 caacatcatc ttaaatcaac gggttagtgt tgctgctcct gtcttttatc aacgtaaaga
    15301 cagaaggaca atcgtcatga acaatcaacc gaataccgat actgacctga tgaatgacaa
    15361 actggtgacg atggcattta ttaccacgat tactggcctg accgacaaat ggttttataa
    15421 actgattagt gaaggcaagt ttccaaagcc tatcaaactg gggcgtagtt cccgctggcg
    15481 ggaaagtgaa gttaaacgct ggctgacgga acgtattgca gaatcccgct attaattcct
    15541 ctgatatcat ctccacacgc tttacccgta aacattaatt cagcacaaac gctatatcag
    15601 taagcgcctg acagggccgt tgctgtgcat ctttcggcgc gtaacttcat tatacccgga
    15661 tgaaatacgt ttcatttaac gttgtcgaaa aataattaac cggggaatag cctgcttatt
    15721 tagcaggaat tccccggatg ctgctaaagg taaaattagg aattaaatgt ccagctaccc
    15781 gtatcggtca caatgtcagc ttcaatgata cggtcacact tcatatattt gaacgtagcc
    15841 acatcaccga atttatattt agctggcagg gttctgttac cgacgctctc cattttacag
    15901 ttccctctgt ttacccgaat ggatttcagc accacgttgt catcagtgat agtgagctga
    15961 ataacaccat atctggtatt ccagccttca ttgctgacag agctgccgaa ctgaacttta
    16021 aatggtggtg tcgtagattg ctgtgcctgc tgttgttgag agggagcagg tttattagcc
    16081 tgctggctac caacgccatt aatgccattc tgaaggaggc tcctggaaac cttaatggta
    16141 cccggcttgc atgatactgt gctggtttcc tgccccattg cattcatggc agtgtaataa
    16201 gcatcacctg taccatcata ttctgagtaa ttgacggcaa ccgcgccttc gatatcttca
    16261 ttttctccac gtaatgtgaa agaagcaatt cctgctcccg gattagccgc cagagtctgt
    16321 ttcgcttcgc cggaaggaat atccagtttg ataacggaac caccttcttc ggagtcacca
    16381 cctggcgttt caaaactgat ataaacggta ttgctgacgt ggctgagtgt catttgctca
    16441 ccgttatcaa gcagacattg caggtcatag gccccgacag cataagcagg gtggaatgca
    16501 aataaagata caaacaatac aggtataatg attggtttca ttaagcactt tccctatcca
    16561 aaaaaatggt cccaggcatt accagcccac tcgataagtt tgtctttccc ctttcgggta
    16621 agctctttga caggctttgg taacggaaaa gaatcaataa cgtcgtcgag tatcccggag
    16681 attacgctgc caaagccgtt ttttgcttcc gtctttactt cgtcggtttt gaactcatcc
    16741 ttaacctgcg aagccgtgct acttttagcc cgatcaggca aagcccgaat aatagtctct
    16801 accagacgaa gaatgttgta gccctcatcg gcggagacag gtgtcaccgg atagtgcgta
    16861 aaaccaaatc gttcagtaat gtaggcttct ttcttttcga tgttgatgcg ctgtgcagga
    16921 gatggctgat gcagatgagt atcccactcg cggaatggct caattttatc aacctggtta
    16981 aggacgaata aaacgcgctc acccccacca gcagggagca gaacattgtt gtagaaatgc
    17041 tcatccgcag aaaaggcccg atcgtctcct tttattaccc acaggataag atccagggag
    17101 ggaagcaaat tgcggtataa atcttcatac tccttatccc gccgggcatt ctcaccgaca
    17161 ccaggtatat caatgatctt tagcgaatgt ttgccgaagc gtatacgcaa ttcctgaact
    17221 tcgcgagtac atgcctcaac gtcgctaact gcacaaacct cccctttaaa cagcgcatta
    17281 catacgctgc ttttccccgc gcctgttttt cccatgacac caatcacggg ttcatagtcg
    17341 atggcttcac tgattttgtt caacagcttt tcccgcagtg gctgagggaa aatatcaaga
    17401 atatccctga tgtcgggtat gtgcggtttg ctatcacctt tttgctcgtt gctaccatta
    17461 cgttctggaa atatattgcc ccacattcca aaaatcccgc cttcccttct ggtgacgcca
    17521 ctggcatcag taatcatccg gccttcgccc tttaccgggt catccatacg aggtctcctt
    17581 ttttaatttg aaataaacgt tgtctgttac tctctgaagg tctgaattac gtcacttatg
    17641 aaacgaaagt gtgggagggc tgaaatgaac tcttcactgg cagtcagagc atcaagttca
    17701 ggctttctgc gaaaagaagt atccagaacg gttacagcgt taacgtcttc catccgaaac
    17761 acctgtagat ttctggtgcg ggtagcaaca agaaaccagt ttttggactg aaatatcaga
    17821 cggcggggag ctactgcttc gtagctttta ccgttaacta ataatgtaac ggcgcggtag
    17881 tcgcggatag cttccgccag ttccagaaaa caggcggttt gtgtaactgg agatggcagc
    17941 cctccatgcc ctataaggca cggtgagctt ccggagttac tggtaatcag tcgaattaac
    18001 ctgcgattct gagccggaat aatccccgaa atcccggaat tacggataaa cgcaaaggtg
    18061 ttgggtgagt gctcccggcc cgttcggcgg gtaagtcggt agcttccgct ggaatgctcg
    18121 agatccagat ggattaggcg ttgctgaaaa tcccgctgta gggtgcgctc tgaaacacca
    18181 aactcatctg ctaacgcttt aagctccaga gattcgccag ccagtaagcg ggtgataatg
    18241 actgatagcc tgacagacag gcggtcataa tgatcatgca gtgttttcat ggtggggaca
    18301 gtcctgttct cccaaaatat catggtattg atacgggaga ttatgccgta caggtacgac
    18361 actttatgtc gtaccagagg ggagtggata gaaaaaatgg cgaagatgcg aagagttgat
    18421 taattcattg aaacaaaatg aattaataat gattttgggt aaaagcgacg tcaaaatacg
    18481 acacaacctg tcgtattttg tgttagccgg gaaaagatat ctgctactta ctaaaatgat
    18541 ttagtgctga aaatatttat aagccaataa atagacaggt attacggagt aacgcccagg
    18601 ctatgttaac gagcctgctg ttttattggc tcaaaaaaca tatcccatag atgatcagcc
    18661 ataccctcgt tagtgagcaa ggtatccctt gagtagccag ggctgaagac gcccattgat
    18721 gggcgaaaac caaggatctg gccgttattg tcaatggtta agatttcatt gtaagcattg
    18781 ccaatcacgc catcctggct ataaccaata tcgcctatcc ccatatttcg cccgctatta
    18841 agccagattg cacattgacc tactttgctt cccccaatgt agatcgcaca agtgaaggca
    18901 tttgcgtcac gcttattaaa atcaacctct aacccggcat ggcgtttttt gagttcagtc
    18961 agcgagcctt caaaataacg ggcgacatat tcaaaaccgt cacggcaggc acgatcttta
    19021 tcaaggtctg taaaatgctt tggcaaagaa agattgctgg aacgtggcgt aaaaacggta
    19081 tccgtcgcct gaaacattgg attagccgga actggggtgt gcacaatctg ttgcggcttt
    19141 ttcgcttcca aattggcaat agcgcgggaa acggcatcga caacctgaac ataaccctca
    19201 tcgtggctgg caaacctggt gataggttta ccatcaattg tcgctgccat aatgctgccg
    19261 aacggtaact gatgccaggc acactctctt aagatcacag gaatgaccac agcctcgccc
    19321 cggttatgac gctccagagc gttggtcatt tctacctgat agcaatagtc agaagcgatg
    19381 aaatcactgc taatcaacaa caggatgata tcagcctgag aaaagtaatg atctatttgg
    19441 ttctcaaact cttgtcctgg aatgatgcga cgatcatgcc atgtcgtgat cttgcccgtg
    19501 cgcttcaggg gggaaagatg cttctcaagc tcattgcgca gtgcttcgtc tgcatgagaa
    19561 taggaaaaaa cgagagaggt catagtgcgt aagatgctcc gatagggagg tggaaaatac
    19621 cgttattttg catctacaag tgtaaaaatg taatccccct tgccgcaagg tattgatgcg
    19681 gaaatcagtc cttcatcttg gtatcactct gacgtcaggg tatgatctct ggcaatttcc
    19741 ccgattttac acaacctgga catcgaacca ggcataaatg attttttgag gttttgatgg
    19801 acaacgctga acagcaattt ctgaacgatc tggatactaa gttctggaaa gccgcagaca
    19861 agctacgcgc caatatggac gcagccaact acaagcatgt ggtactgggg cttatctttc
    19921 tgaagtatgt ttcagatgcc tttgacgccc gccagaagga actgaaagga ctgtttgaag
    19981 acaagttcaa cccggacaac atttacgcgc tgtctcgtga cgattacgac acagaagagg
    20041 cgtaccagca ggcgatcgca gaagagctgg aagttcagga ttactacact gagaaaaacg
    20101 tcttctgggt acccaaactg gctcgctggg aaacgcttaa aaccaatgct gctctgcctg
    20161 tagggacggt tctggggaaa gacgatagcg gcaaagatat cgtcatgacg tcagtatcga
    20221 agctgattga tatcgctctg gatactatcg aaaacagtaa cccgaaactg aaaaacatcc
    20281 ttaaccgtat cgggcattat cagcttggta acgacttgct aatcagcctg attaacgttt
    20341 tctccgatac cagtttcagc gaacctaaat acaatggcgt gaagctcaat ttgaagagca
    20401 aagatattct cggccacgtt tacgaatact tccttggtca gtttgcactg gcggaaggca
    20461 aacagggcgg ccagtattac acgccgaaaa gcatcgttac gctgattgtt gaaatgctcc
    20521 agccctacaa tggccgcgtg tatgacccgg cgatggggtc tggtgggttc ttcgtttcca
    20581 gcgatcgctt tatcgaagcg cacgcagatg aaaagcatta caacgctgct gaacaaaaac
    20641 gcaatatctc cgtttatggg caggagtcca accctactac ctggcgtctg gctgcgatga
    20701 acatggtgat tcgcggtatt gattttaact tcggtaaaca gaacgccgat acgttcctga
    20761 acgatcagca cccggattta cgtgccgatt ttgttatggc taacccaccc ttcaatatga
    20821 aagagtggtg gaatgcgaag ctagaagatg acgtgcgctg gcagtacggt acaccaccgc
    20881 agggtaacgc caactttgcc tggatgcagc acatgatcca ccacctggca ccgaaaggtt
    20941 cgatggcgtt gttgttggca aatggttcga tgagctctaa cagtaatacg gaaggtgata
    21001 tacgccgtca gattatcgaa gctgatttgg ttgaatgtat ggtagctttg ccaggtcaac
    21061 tcttcactaa cacgcagatc cccgcttgta tctggttgtt gacgaaagat aaatctggag
    21121 gtaatggtaa agcgcatcgt aagggtgagg tgctgtttat tgatgcccga cagattgggt
    21181 ttatgaaaga tcgcgtatta cgtgatttct caacggatga tatcaaaaag attgccaata
    21241 ctttccatgc ctggcagaca gataaagatt atgaagatga ggccggtttc tgttttgcag
    21301 ccacactaga cgatataaag aaaaacgatt ttgttctaac accaggacgc tatgt
//
LOCUS       DAJOTB010000078        21050 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJOTB010000078 DAJOTB010000000
VERSION     DAJOTB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.078"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            162..764
                     /locus_tag="OHN28_002843"
     CDS             162..764
                     /locus_tag="OHN28_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCQ2298211.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(803..1228)
                     /gene="nudI"
                     /locus_tag="OHN28_002844"
     CDS             complement(803..1228)
                     /gene="nudI"
                     /locus_tag="OHN28_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCQ2298212.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1507..2049
                     /locus_tag="OHN28_002845"
     CDS             1507..2049
                     /locus_tag="OHN28_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298213.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            2149..3351
                     /locus_tag="OHN28_002846"
     CDS             2149..3351
                     /locus_tag="OHN28_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCQ2298214.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3571..4353
                     /locus_tag="OHN28_002847"
     CDS             3571..4353
                     /locus_tag="OHN28_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCQ2298215.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4368..5573
                     /gene="rhmD"
                     /locus_tag="OHN28_002848"
     CDS             4368..5573
                     /gene="rhmD"
                     /locus_tag="OHN28_002848"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCQ2298216.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5630..6919
                     /locus_tag="OHN28_002849"
     CDS             5630..6919
                     /locus_tag="OHN28_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298217.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6937..7740
                     /gene="yfaU"
                     /locus_tag="OHN28_002850"
     CDS             6937..7740
                     /gene="yfaU"
                     /locus_tag="OHN28_002850"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCQ2298218.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(7781..8014)
                     /locus_tag="OHN28_002851"
                     /pseudo
     CDS             complement(7781..8014)
                     /locus_tag="OHN28_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8015..9010)
                     /locus_tag="OHN28_002852"
     CDS             complement(8015..9010)
                     /locus_tag="OHN28_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCQ2298219.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(9203..10393)
                     /gene="glpC"
                     /locus_tag="OHN28_002853"
     CDS             complement(9203..10393)
                     /gene="glpC"
                     /locus_tag="OHN28_002853"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCQ2298220.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10390..11649)
                     /gene="glpB"
                     /locus_tag="OHN28_002854"
     CDS             complement(10390..11649)
                     /gene="glpB"
                     /locus_tag="OHN28_002854"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCQ2298221.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11639..13267)
                     /gene="glpA"
                     /locus_tag="OHN28_002855"
     CDS             complement(11639..13267)
                     /gene="glpA"
                     /locus_tag="OHN28_002855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCQ2298222.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13540..14898
                     /gene="glpT"
                     /locus_tag="OHN28_002856"
     CDS             13540..14898
                     /gene="glpT"
                     /locus_tag="OHN28_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCQ2298223.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14903..15979
                     /gene="glpQ"
                     /locus_tag="OHN28_002857"
     CDS             14903..15979
                     /gene="glpQ"
                     /locus_tag="OHN28_002857"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2298224.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            16182..16832
                     /gene="inaA"
                     /locus_tag="OHN28_002858"
     CDS             16182..16832
                     /gene="inaA"
                     /locus_tag="OHN28_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCQ2298225.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16886..17140)
                     /gene="yfaE"
                     /locus_tag="OHN28_002859"
     CDS             complement(16886..17140)
                     /gene="yfaE"
                     /locus_tag="OHN28_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCQ2298226.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17140..18270)
                     /gene="nrdB"
                     /locus_tag="OHN28_002860"
     CDS             complement(17140..18270)
                     /gene="nrdB"
                     /locus_tag="OHN28_002860"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCQ2298227.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18359..20644)
                     /gene="nrdA"
                     /locus_tag="OHN28_002861"
     CDS             complement(18359..20644)
                     /gene="nrdA"
                     /locus_tag="OHN28_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCQ2298228.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4910 a         5165 c         5629 g         5346 t
ORIGIN      
        1 ttatattttt tcttatttag actttgcatt tggcaaaatt ttgaggcatt ttgccggcat
       61 cataggattt ttaatattac accaactgct aattatcgcc agaaaagtcg ctcaatttca
      121 cggtaattgt ctggttgcgc ttgtctacag gtggagttta cgtgttagct ttttgccgat
      181 cttcgttgaa gtcaaaaaaa tatttcatca ttttactggc gctcgctgca attgccggac
      241 tggggactca tgccgcctgg agtagcaatg gtttgccacg catcgacaat aaaacactgg
      301 ccagactggc acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa cgttgcgatc
      361 gttcaaccaa tcaatgcttg tcagataaaa caggtattac ggtcaaaggt acccaggatg
      421 cccgtgaact gggcaacgct tttagtgctg atatccctga ttttgatctc tattccagta
      481 ataccgtccg gactattcag tcggcgacct ggttttcggc gggtaaaaag ctgacggtag
      541 ataaacgatt tcttcaatgc ggtaatgaga tttatagtgc aattaaagac ttacaacgca
      601 aagcacctga taaaaatatc gttattttta cccataatca ttgcctgaca tatattgcta
      661 aagataagcg tgatgcgaca tttaaacctg attatctgga tggtttagtc atgcatgtgg
      721 aaaaaggcaa agtttatctg gatggggaat tcgttaacca ctaaattttc aatctgacag
      781 ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa acgtaacgtt tttcgggtgg
      841 caacattcaa atcataatgc accagatctt caggttttac ccacgcgtag tcctgaaact
      901 cttcgtttat tttcacatct cggttggcag aaacgcagtc aaaaatcagg taaatcatat
      961 aaatctcttc cttgcgacca tctgcatacg tcttggtgcg aatatcatcg ctgaaggtcc
     1021 acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt tcgcggcgta
     1081 gtgcctcttc aattcgttcg ccaggctcca cgccgccacc cgaaagcgcc cattgaccgg
     1141 ggaaaacgcc gcgatcgtcg gccattttac acagcaaata agcaccatca ttttgaatca
     1201 aagggcatac aatagtccgt tgtcgcacag gggtattcct tataaataaa aagaaaccat
     1261 gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc gatgataact
     1321 acatttatta ttatttttgt gtgtatcgat aaagtgtgtt tattttttcg gcgtttaatt
     1381 atttaagaag tatttagtta caataagtga ctagatccct tttttcgctc attgaatcct
     1441 ctgcgccagg cagtacaatc ccgcagcttt gggaattttg attaattgct tgtgggaaag
     1501 aggataatga aaaaaattgc gctagcaggt ctggccggaa tgctgttggt ttctgcatcg
     1561 gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat acaccaatat tggtgtcggt
     1621 tttgggactg aatcgacggg cctggcgtta agcggtaact ggacacataa cgacgacgac
     1681 ggtgatgccg cgggcgtggg gctggggttg aatctgcctc tcgggccatt aatggcgacc
     1741 gttggcggaa aaggcgttta caccaacccg aacgatggtg atgaaggtta tgcagcggca
     1801 gttggcggtg gtttgcagtg gcaaattggt gacagcttcc gcttgtttgg tgagtattac
     1861 tactctccgg attcgctctc cagcggaatt aaaagttatg aagaagcgaa tgctggcgcg
     1921 cgttacacca ttatgcgtcc agtcagcatt gaggctggct accgttacct gaacctggca
     1981 ggtaaaaatg gcaatcgtga taacgccatt gctgatggtc cgtatgttgg ggtaaatgcc
     2041 agcttctgac gccttccggc gcggcgttct gtcgcgccat tcagtttctt actacgccct
     2101 gtctcccttt gcgttatagt gattcgataa caaaagcagg agataaccat gttaaaagtg
     2161 gaaatgttat ccaccgggga tgaagtgtta cacgggcaaa tcgttgacac taacgctgcc
     2221 tggctggccg attttttctt tcatcagggg ctgccattat ctcgccgcaa tacggtgggg
     2281 gataaccttg atgacttagt caccattctt cgcgaacgta gtcagcacgc cgatgtgttg
     2341 atcgttaacg gcgggctggg accgaccagt gatgatttaa gcgcactcgc cgctgcgaca
     2401 gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca aagagatgga acgctatttc
     2461 catgaacggg gacgagtaat ggcaccgagc aaccgtaaac aagcggagct gcctgccagt
     2521 gctgaattta tcaataaccc ggtaggcact gcctgtggtt ttgccataca gcttaatcgt
     2581 tgcctgatgt tctttacccc cggcgtaccg tcagaattta aggtgatggt cgagcacgaa
     2641 atcctgccgc gcctgcgcga gcgtttttct ttaccacagc cgccggtttg cctgcgtttg
     2701 actacctttg gtcgttcgga aagcgatctg gcacaaagcc tggacactct acaactgccg
     2761 ccgggcgtaa caatgggcta tcgctcctca atgccgatta tcgaactgaa actcaccgga
     2821 ccggcaagcg aggaacaggc gatggaaaaa ctgtggctgg acgttaaacg tgttgccgga
     2881 cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc agatcagtcg cgaattgcag
     2941 agccgccagt tcagcctgac gctgagcgag caattcaccg gtggtttatt ggctttgcaa
     3001 ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg ttccttcaca ggaggaaacc
     3061 ctggcgcaaa ctgcgcactg gattacagaa cggcgggcga accattttgc tgggttggca
     3121 ctggctgttt ctggtctgga agatgagcat ctcaactttg cactagccac gccagacggc
     3181 actttcgctt tgcgtgtaca tttcagtgcc acacgctaca gtctggctat tcgccaggaa
     3241 gtgtgcgcaa tgatggcgct taatatgcta cgccgttggt taaacggcca ggacatcgcc
     3301 agcgagcatg gctggattga ggttgttgag tccatgacct tatctgtctg attccgccac
     3361 tctgtgccgg tttactgcac cggcacattc cccctccggc aaaatttgtc caacctgttg
     3421 tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt gtgcgtaatt tctccagtcc
     3481 gctggacgag aaacgggatt tgatgcgctt cacaatttta ctccccctga acaacgagac
     3541 tgattccagt atctaagact ggagtccagt atgttggaat caagcaaagt tcccgccctg
     3601 acacgggcta ttgatattct caatttgatt gcccggattg gcccctgtag tgctgcgacg
     3661 atcattgaca cattggggat ccctaaaagc acagcctatt tgctgcttaa tgaactcagg
     3721 cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt gtttgtggac caggctggtg
     3781 gagctttccg gtcatgcgtt aagcaagatg gatctccggg aactggctcg accgcgtttg
     3841 acgcaactga tggataccac cgggctgtta tgtcacctgg ggattattga taacggcagc
     3901 gcttattaca ttttgaaagt ggagtcatca gccactatca gcgtgcgttc ccatgaagga
     3961 aaaagccttt cgctttatcg ctccggaata ggtaaatgcc tgcttgcctg gcaacctgca
     4021 gctgtacagc aaagtattat cgaagggctg gtctgggaac gggcgacacc cacaacgatt
     4081 actcatccac aacggctcta cgaagaactg gcgcgtattc gccgccaggg ctggagctat
     4141 gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg cgccggtgtt taacgccaat
     4201 aacgagctta ccgctgcgat ttcggtggtc ggtacccgct tacaaatcaa tgaagaatac
     4261 cgtgattatc tggcgggcaa ggccattgcc tgtgccaggg atatttcacg tttgttggga
     4321 tggaaaagtc cctttgactt acaggcctca taacaatgga gaacatcatg accctaccaa
     4381 aaatcaaaca ggttcgcgcc tggtttactg gcggtgcgac agcagaaaaa ggcgctggcg
     4441 gcggtgatta tcacgaccag ggggcgaatc actggattga tgatcatatt gccaccccga
     4501 tgagtaaata ccgcgaatac gagcagtccc gccagtcatt tggcattaac gttcttggca
     4561 cgttgattgt tgaagttgaa gcagaaaacg gccagaccgg attcgctgtt tcgacagccg
     4621 gtgaaatggg ctgttttatt gtcgaaaaac atcttaaccg tttcattgag ggtaaatgcg
     4681 tcagtgatat caaactgatc cacgatcaaa tgctcaatgc caccctgtat tactccggct
     4741 ctggtggcct ggtgatgaat acgatttcct gtgtcgatct ggctctgtgg gatctgttcg
     4801 gcaaagtggt cgggcttccg gtttataaac ttttaggcgg cgctgttcgt gatgagattc
     4861 agttctacgc cacaggtgcg cgtccggatc tggcaaaaga gatgggcttt atcggtggca
     4921 aaatgccgac gcactggggg ccacatgatg gcgatgcggg gatccgcaaa gatgccgcta
     4981 tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg gttaatgctc gactgctgga
     5041 tgagtcagga cgtgaattat gccaccaaac tggcccacgc ttgcgcgccc tataacctga
     5101 aatggatcga agagtgcctg ccgccacagc agtatgaagg ttatcgcgaa ctgaaacaca
     5161 acgcaccagc ggggatgatg gtcaccagcg gtgagcacca cggcacactg caatctttcc
     5221 gtacgctttc agaaaccggt atcgacatta tgcagccgga tgttggctgg tgcggtggct
     5281 taaccacgct ggtggaaatt gccgcaatcg ccaaatccag ggggcaactg gtggtgccac
     5341 acggttcgtc tgtttactcc caccatgcgg tgatcacctt caccaatacg ccattcagcg
     5401 aattcctgat gaccagcccg gattgttcaa cgatgcgtcc gcaatttgat ccgattctgc
     5461 ttaatgagcc agtaccggtg aatggtcgta ttcataaatc agtgcttgat aaacccggtt
     5521 tcggcgtcga actcaatcgt gactgcaatt tgaaacgccc ctacagccac taatcactcc
     5581 gggcgttgct gtgacaacgg taacgcccac atattctaag gagcttttta tgagcaccgc
     5641 tttgcttgac gccgtggtga agaaaaaccg cgcgcgttta attccgttta tgttggcgct
     5701 gtatgtgctg gcgtttctcg accgttcgaa tattggtttt gccaaacaga cctaccagat
     5761 tgataccggg ctgagtaatg aagcttatgc gctgggagca ggcattttct ttgtggtata
     5821 cgcgtttctg ggggttccgg cgaatctttt gatgcgcaaa ctgggggcca gaacctggat
     5881 tggtacgaca acactgctgt ggggatttct ttcggctgcc atggcatggg ccgatactga
     5941 agcgaaattt ctgatcgttc gcactctgct tggtgctgcg gaggccgggt ttttccctgg
     6001 tatgatttat ctcacttcgc aatggtttcc gcagcgtaat cgcgccagca ttatggggct
     6061 gttctatatg ggcgcaccgc tggcgttaac actgggatca ccgctttccg gcgcgctgct
     6121 ggagatgcat ggatttatgg ggcatcccgg ctggttctgg atgtttgtga ttgaaggatt
     6181 gctggcagtc ggcgctgggg tattcacatt cttttggctt gatgacacac cggggcaggc
     6241 acgttttctg agtaaacaag aaaaaacgtt gcttatcaat caactggcaa gtgaagaaca
     6301 acagaaagtg acttctcgat tgagcgatgc gctgcgtaat gggcgagtct ggcaactggc
     6361 gattatctac ctgaccattc aagtggcggt ttacggatta attttcttcc tgccgaccca
     6421 ggttgctgca ttgctgggga caaaagtggg ctttacggcg tcggtggtca ccgccattcc
     6481 gtgggttgcg gccttgtttg ggacctggct tattccgcgc tactccgata aaaccggcga
     6541 acggcgtaat gtcgcagcgc tgacattact ggcggcgggc attggtattg gtctctccgg
     6601 gctgctttct ccagtactgg cgatcgtagc actgtgtgtt gcagccatcg ggtttattgc
     6661 cgtgcaaccg gtgttctgga cgatgccgac acaacttctt tccggtacgg cgctggctgc
     6721 ggggattggt tttgtaaacc tgtttggtgc agtgggcggg tttattgccc cgatcctgcg
     6781 cgtgaaagca gaaacgttat tttccagtga tgcggcggga ttactgacgc tggcagcggt
     6841 ggcggtcatc ggttcgctga ttattttcac tctgcgtgta aatcgcactg ttgcgcagac
     6901 cgacgtggca catcattaaa tagtttaagg aacacgatga acgcattatt aaccaatccc
     6961 tttaaagaac gtttacgcaa gggcgaagtg caaattggtc tgtggttaag ctcaacgact
     7021 gcctatatgg cagaaattgc cgccacttcc ggttatgact ggttgctgat tgacggggag
     7081 catgcgccaa acaccattca ggatctttat caccagctac aggcggtagc gccctatgcc
     7141 agccaacccg tgatccgtcc ggtggaaggc agtaagccgc tgattaaaca agtcctggat
     7201 attggcgcgc aaactctact gatcccgatg gtcgatactg ccgatcaggc acgtcaggtg
     7261 gtgtctgcca cgcgctatcc tccctacggt gagcgtggtg ttggggccag tgtggcacgg
     7321 gctgcgcgat ggggacgcat tgagaattac atggcgcaag tcaacgattc gctttgtctg
     7381 ttggtgcagg tggaaagtaa aacggcactg gataacctgg acgaaatcct cgatgtcgaa
     7441 gggattgatg gcgtgtttat tggacctgcg gatctttctg cgtcgttggg ctacccggat
     7501 aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg gatccgtgct
     7561 gcgggtaaag cggctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg
     7621 tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat
     7681 caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcgtaaaagg tagttattga
     7741 tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca
     7801 gatcggcttc gcttagctga gtggccgcga gcacctgatc acgatcaatg ccctgttcca
     7861 gcatgcgcaa agcaatttta atggcttgtt catgcatgcc ttcctgcaaa ccttctaatt
     7921 taccttcttg ccagccttca atcttaccct cctgccagcc aatttgatgt ccttcctgcc
     7981 gtagccgttc agcaatagtc atcaatctct ccttttaatg acagttcgga atatcagcat
     8041 cagtgagttg ggttgccgcc agcacaatct cacgttcaaa gccctgctcc agcatgcgca
     8101 aggcaatttt aatggcttgc tcatgcatgc cttcctgcaa accttctaat ttacctttct
     8161 gcaaaccctc caatttacct tgctgcaaac cttctaattt accttcttgc cagccttcaa
     8221 ttttaccttc ttgccagcca atttgatgtc cttcctgccg tagccgttca gcaatagtca
     8281 ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaag cgggtgaaac
     8341 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     8401 tgaagtctct aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     8461 gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg atctcatcgt
     8521 caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     8581 gcggatcgtc aaactcatcc agccagttga gtgagtacgg gtagggcgag gcttcgccat
     8641 gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc aggtgacgct
     8701 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     8761 gatgttcaat cacgcagtag atatagccgt caccctcgct ggtttccacg gaccacaaaa
     8821 catccgagta gtaagcgcgt aaactttttt caataaaact ggtgggttcc aggcgtaagg
     8881 tttgcaggtt gcaaagcttg cgcagcggct ctggtagatg tatttccaga aaatcccgtg
     8941 cggtttcggg tgtgaacata aaggttttaa acaccgcatc atgcggggag gaggttgttg
     9001 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     9061 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     9121 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aacaacatgc
     9181 ttaccgggca tcagaaagga gtttaagcca acgcctgtgc cagcagcgta atcggatgtt
     9241 cgcagcgaag acttgtggac atctcaatct gccatttgca ggtttcgcag tcggtgacca
     9301 ccagatctgc gccgctctct tctatctggc ggaacagtgg tgcgccgatg gcttgtgaag
     9361 tggggtagtt ctcttttttg aaaccgtaag ttcccgcaat accgcagcac tgggaatcca
     9421 gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc
     9481 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     9541 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt tcgatatgat
     9601 cgcgcaagcc tttgttgtcg acattcagca cttctgggta ttcgtcgcgc agggcaaatg
     9661 tacacgttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     9721 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaaaccgttg gcgatcagcg
     9781 gtacgccgca gcatttttct ttactgagca gttgtacgcc ggtatccatc gcgttgagca
     9841 ctttaattaa atctttgcct agttgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     9901 aagcgacctg gtctttatac tgcgcttgct gagccgccac gctgcgatac cagcgacgga
     9961 acgtgccgaa ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac gccgcatcaa
    10021 gcagctgacg caccggtttc agcgaggtag cggtgttgac gatgggtgcg aacggcgtgg
    10081 atacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
    10141 tgtcatattt cgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg
    10201 cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat
    10261 ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccccgga taacccggat
    10321 tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
    10381 tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
    10441 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
    10501 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagtgcctc
    10561 atcagtggtt acaccgaact gctgccacgg ctgcggcgca aaaaaatcgc ccttatacca
    10621 ttcaccccgc gtagcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtc
    10681 acgttccgct accagtccgc cgctaaagaa actgccgctc gcaagcaccg caaatcgtgg
    10741 gcgcagcggg atatcggcgt gattgcgcgt ccagatttca ttcactacac catttttaca
    10801 ggtcactttt ttcacttcat cgcccggcat ccacacgccg ccctggcgca caaactggcg
    10861 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
    10921 caatgaacaa ggtagttttt cattcaacca atgccacagt ttgtcatcgg ccaggccgaa
    10981 gcaggcgggc atcaggatca tttcgcaggt attggcgacc ggaataagcg catcaagtaa
    11041 caacggccag ttttcttcat tatcaaggaa gcgggcaata ttcaccgccc ggaactcggt
    11101 ggcgttattg cgcagcacat ccagttccgg cagttcaatt tcagcggttt caactttaag
    11161 atccaattcg cgcaatgaag ccgccgcaag atgcgcctga aaatccatca ggccgctaat
    11221 tcccactaca catattttct tcgcgggcag cggccagaca gggacttctg gcgaacttag
    11281 ccaggttgag cgcagagtgc cgagcggcgt aatccgctgg tgagccagtt ctacgctgcc
    11341 ctgcaattgc gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga gatcgagcac
    11401 gcgttgaggc ccgagaagag tgtaaggatg ggcaggtgcc tgctgacgca aagattccag
    11461 tccactgtga atgtctgtca ccggttgacc atccggcaga tggctcagca aatccagcga
    11521 tccagatgag aaatggagcg cgctttgacc acgagtgaca atggcacagc gcaggccgtg
    11581 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
    11641 aaagcgcatc tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa
    11701 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    11761 ttaggaaggt ggaaagttgc tcaatagatt gcgcggacgt cgtgacgtta aaacgttgca
    11821 gcagtccggc agcgcggcag gcgcagagct cgccctggca ggtgcccatc cccacacggg
    11881 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    11941 cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca
    12001 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggta
    12061 gggagatgac tttacgcaag gtaacttcag cgggttcctg tgaaccagga agtgccagat
    12121 cggcggtcgt acaggggcgc gtgttgccca gtttgcggca tacggcatcg gtagcccatt
    12181 ccgccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaac ccgtccagac
    12241 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcggat
    12301 cgtcatcgct ggcaaccagc gggcgtacgc cagagtaggc ccgcaaaatg cgcgttttcg
    12361 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacttct tctgccgtta
    12421 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa
    12481 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt gcggcagcgg ttgatcacat
    12541 gctgattaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
    12601 gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta actacgacag
    12661 gtgcatgaag tgcctgaatt tcgccggtga gatggttacg tacacgaacg ccgcacaccg
    12721 ccgcgccttc acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt
    12781 ctttggcatc cagcatgttt gctgctgtca gacgaaatgg atcaacggtg ccatccggaa
    12841 ctttcaccgc gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
    12901 gatcaatggc ttctgcgctg atccctgctt cttcgcaggc gcggataaaa gtggcctgga
    12961 aggagaggtc atcttccggc agggtgataa acaggccgtt ggttggttct acgcagtgac
    13021 gtgcaatgcg tttcaggatc tggttttcac taatacattc gcgggccgat tccgcatcgg
    13081 ttaccgcata gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg
    13141 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
    13201 tcccggctcc cgttgcgcca ccgccaatga taatcacgtc acttgattgc gagtcgcgag
    13261 ttttcattgt tatccctcag aagttcgttt tttaccattt agccatagta aaaacatgaa
    13321 ttgtttgatt tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca
    13381 ttctgaacat ttcaaccatc gtatttaata atgtgtgcgg taattcacat ttaatttatg
    13441 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctcccact gaatcaggct
    13501 gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
    13561 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
    13621 tggcaaattt tcctggggat attctttggc tatgctgctt actatttggt tcgtaagaac
    13681 tttgcgctcg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
    13741 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
    13801 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
    13861 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg
    13921 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
    13981 tggtggtcgc agaaagaacg tggcggtatt gtgtcagtgt ggaactgtgc gcacaacgtc
    14041 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcac
    14101 gcggcgctct atatgcccgc tttctgcgcc attctggtgg cactgttcgc ctttgcgatg
    14161 atgcgcgata ccccgcaatc ctgtggcctg ccgccgattg aagagtacaa aaatgattat
    14221 ccggacgact ataacgaaaa agcggaacag gagctgaccg cgaagcagat cttcatgcag
    14281 tacgtgctgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg
    14341 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    14401 ctggataaat cctcctgggc ctactttctt tatgaatatg caggtattcc gggcaccctg
    14461 ctgtgcggct ggatgtcgga taaagtcttt cgtggtaacc gtggggcaac cggcgtcttc
    14521 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    14581 accgtcgata tgatttgtat gattgttatc ggcttcctga tctatggccc tgtgatgctg
    14641 atcggtctac atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggtttt
    14701 accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    14761 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg
    14821 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa
    14881 aaacgaaacg gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat
    14941 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    15001 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    15061 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    15121 tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc
    15181 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    15241 gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc
    15301 ggggcgtttc ccaatgggta aatctgactt ccgggtgcac acctttgaag aagagattga
    15361 atttgttcag gggttaaacc actctaccgg gaaaaatatc ggtatctatc cagaaattaa
    15421 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct
    15481 gaagaaatat ggttacaccg gtaaggacga caaagtttat ttgcaatgtt ttgatgctga
    15541 tgagctgaag cgtattaaga atgagctgga acctaaaatg ggtatggatc tcaatctggt
    15601 acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg
    15661 ggttaattac aattacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata
    15721 tgctgatggt attggtccgg attaccatat gttgattgag gagacatcgc agccgggtaa
    15781 tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta
    15841 taccgtgcgg tcagataaac tgcctgaata cactactgat gtgaatcagt tatatgatgt
    15901 tctgtataac aaagcgggtg taaatgggct atttactgac ttccctgata aggcagtaaa
    15961 attccttaat aaagagtaaa ttaactaccg gatggcactt atgtgctatc cgataccact
    16021 cttcattaaa gtctgtgggt tatccttaat tgcaattatt tagagcgaga tgacttgttc
    16081 ccggcataag atactcgtca atacaacacg tattattaag attttcctca gctaaacatc
    16141 tatagtcatt gtcgttaata aaaaacgaca ggcgataggg tatggcagtt tcagcaaaac
    16201 acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc
    16261 gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa
    16321 agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga ccaacgattg
    16381 tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagattg
    16441 tttttggtga agcggtgaaa attgagggtg aatggcgcgc gttgctggtg actgaagata
    16501 tggcggggtt catcagcatt gctgactggt atgcccggca tgcagtatcg ccttattctg
    16561 acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg
    16621 ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg
    16681 cagaagctgg atttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgt gataaagcga
    16741 ttaatcatga cttccgccag ctggaaaaat atctggagcc gatccccaaa gcggactggg
    16801 agcaggtgaa agcatattac tacgctatgt aatcgttgta ggccagataa gacgtataag
    16861 cgtcgtatct ggcgttaaaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc
    16921 aacaacaggg caaaatttcc cccggctgaa taaaagctaa cggttcggca atccagtcca
    16981 cctggcctgc gaccagtcgg gtgcgacagg agccgcagta accttcgcgg cactggtact
    17041 caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca
    17101 gcagttgtgt gccagtaatg cgcagggtaa cgcgggccat cagagctgga agttactcaa
    17161 atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc
    17221 ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccacg ggatcgggtt
    17281 ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta
    17341 ttcaacgtac tggcagagaa tgtctttatt cagaccaatc atcgaaccat cacggaacag
    17401 ataatccgcc cagtctttct cttgctgtgc cgcctggaca aacaggtcat agcactcctg
    17461 cttacattct tcggcaattt ccgccatctc cggatcatcc gcgccgctgc gcagcagatt
    17521 cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat
    17581 tttggcgtta ccttccatca gctcacgttc agcgaatgcg aaagagcagg caaagctgac
    17641 gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt
    17701 cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag
    17761 cagatgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccctccgc
    17821 acgtttctgg atctgctcgt tggtaacgat atcgtcaaag acaacagacg gatcgttaac
    17881 gatattacgg atgatatggg tataggagcg ggaatgaatc gtttctgaga acgcccaggt
    17941 ttctacccag gtttccagtt ccgggatcga gatcagcggc agcagtgcca cgttcggact
    18001 gcgaccctga atggaatcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt
    18061 ttcgtgttca ggcagtgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg
    18121 acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg
    18181 atcgtagcga gccacgttga ccggctgacc aaagaacatt ggttctttga gctgatcatt
    18241 tttcgtctgt gaaaaggtgg tatatgccat gaatgtgtcc tgtggtttta ttacccctca
    18301 cccgtcccct ctcccgcaag gggagagggg acggtttggt gcaaagactg ggggattatc
    18361 agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggcacc agatcgtcct
    18421 gcgcgtcttc tgcgccgtca cgggtgttct gataatacag cgttttgacc ccgaatttgt
    18481 aggcagtgag caggtctttc agcaactgct gcatcggcac ttttcctgac gggaaacgtg
    18541 acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc atgataccca
    18601 ccagttgcag gtagccatcg ttacccggca tttcccacaa cagctcatag gcgtcgtgca
    18661 gatgctcgta gtccggcacc acctggcgca gaataccgtc tttcgacgct ttgatgctga
    18721 cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgcgaa gaagtctcgg
    18781 acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt gactcacgca
    18841 gtgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct ttcttatagg
    18901 tatcgatcgg caggatccct ttcgcgtatg tggtttcgtt aaaccacggg cacgcgcctt
    18961 gctctttcgc cagctcatta gacgctttca gcaggtaata ctgaatggct tcgaaggttt
    19021 tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc gccaggtagt
    19081 aagcgaagtt gatcacacca atacccagcg tacgacgacc catcgctcca cgtttggcgg
    19141 ccgggatcgg gtaatcctga taatccagca gcgcgtcgag tgcgcgtact gccagaattg
    19201 ccagctcttc cagttcatcc aggctgttaa ttgcgcccag gttgaaagca gacagcgtac
    19261 acagtgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc ggcagggcga
    19321 tttccaggca caggttagac tggcgcactg gcgcgatggc cggatcaaac gggctatggg
    19381 tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt tcctgcatca
    19441 tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg tctttctcat
    19501 atttggtata cagacgttca aactcttcct gatcggcgaa gaacgcgtcg tacagccccg
    19561 gtacgtcgga cgggctgaac agagtgatat cttcaccttt cagcagacgg gtatacatca
    19621 gtttgttgat ttgaaccccg tagtccatat gacgcacgcg gttgccttcc acaccacggt
    19681 tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag aacagcgttg
    19741 ccgcaccgcc gcgcacaccg ccctgagagc aggatttcac cgctgtctgg aaatgtttgt
    19801 agaacggaat acaaccggta tggaacgctt cgccaccgcg aatcgggcta cccagcgcac
    19861 gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtattta acaatcgcgc
    19921 tggaggtggc gttaatggaa tccaggctgt cgccgcactc gatcagcacg catgagctga
    19981 actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa attttaaatg
    20041 tggaaaccgc gtcgtaaaaa cgcttcacgt attgcaggcg cgtttcacgc gggtagttcg
    20101 agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag atttcgccgg
    20161 tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca taagagaagg
    20221 tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct tccgtgtagt
    20281 cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccaca tggtcgtaca
    20341 gcgcaggcgg ctcaaactga ccgtaggctt ttttacgcag gtggaagatc gccagacgtg
    20401 cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggca gccttgataa
    20461 tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg gagcgcagct
    20521 cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga acgcgatgga
    20581 ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc agattctgat
    20641 tcatgtatgt cgtacctgtt tttcaatagc ttcatgcaga ctacctgtag tgagggaagc
    20701 tgctaagaac actatatata gggggtatgt ttgggattca ctgcaagata gtgtgaaact
    20761 ggccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt ggcttccgcg
    20821 caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat ttgttaaaaa
    20881 taaccgttcg gtgaattctt gaacggtaaa ataggatagg taaattgata tagatggttt
    20941 attaatcttg ttgctaatga ttgccaaaat tgatctaggc acaagtcgtc acataatctg
    21001 atgtatctgt tgtggtcaat atataagcga atgtaagcga ttgttgagtc
//
LOCUS       DAJOTB010000079        20973 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJOTB010000079 DAJOTB010000000
VERSION     DAJOTB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.079"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            38..898
                     /locus_tag="OHN28_002862"
     CDS             38..898
                     /locus_tag="OHN28_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298229.1"
                     /translation="MHTHPHLIKQTLVPRLINEINKRTQSNSEKLSNSDKLTYINDNY
                     CEITRNYTSFYGMNILSIVLFLPIVILCFGSVLFFIYDIIFNFERYLDTWRYRADSEL
                     LLHIAYCAIFFMSFVFAAILNYFIFAPRHYPIRFNRKTGKVYICNYILNDMSHKSTYT
                     FWWHYPFYKHTKPEYKEYDWEQIQGIAFFAQNHHSTYATIRCIVYESLDSTNIIDTFN
                     LITTESATNALHMTNNKLWLWINSFMNFDDEFLEKSINTAPGIWGREVNWPDDMNKKS
                     MLSSMDEYIL"
     gene            complement(964..1254)
                     /gene="ilvN"
                     /locus_tag="OHN28_002863"
     CDS             complement(964..1254)
                     /gene="ilvN"
                     /locus_tag="OHN28_002863"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2298230.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1258..2946)
                     /gene="ilvB"
                     /locus_tag="OHN28_002864"
     CDS             complement(1258..2946)
                     /gene="ilvB"
                     /locus_tag="OHN28_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCQ2298231.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3052..3150)
                     /gene="ivbL"
                     /locus_tag="OHN28_002865"
     CDS             complement(3052..3150)
                     /gene="ivbL"
                     /locus_tag="OHN28_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCQ2298232.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3551..4735
                     /gene="emrD"
                     /locus_tag="OHN28_002866"
     CDS             3551..4735
                     /gene="emrD"
                     /locus_tag="OHN28_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCQ2298233.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4743..5240)
                     /locus_tag="OHN28_002867"
     CDS             complement(4743..5240)
                     /locus_tag="OHN28_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCQ2298234.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5237..5599)
                     /locus_tag="OHN28_002868"
     CDS             complement(5237..5599)
                     /locus_tag="OHN28_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCQ2298235.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(5589..5936)
                     /locus_tag="OHN28_002869"
     CDS             complement(5589..5936)
                     /locus_tag="OHN28_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCQ2298236.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(5996..7490)
                     /locus_tag="OHN28_002870"
                     /pseudo
     CDS             complement(5996..7490)
                     /locus_tag="OHN28_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(7487..9202)
                     /locus_tag="OHN28_002871"
     CDS             complement(7487..9202)
                     /locus_tag="OHN28_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCQ2298237.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            9369..10235
                     /locus_tag="OHN28_002872"
     CDS             9369..10235
                     /locus_tag="OHN28_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2298238.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(10325..11986)
                     /locus_tag="OHN28_002873"
     CDS             complement(10325..11986)
                     /locus_tag="OHN28_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCQ2298239.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(12184..12612)
                     /gene="ibpB"
                     /locus_tag="OHN28_002874"
     CDS             complement(12184..12612)
                     /gene="ibpB"
                     /locus_tag="OHN28_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCQ2298240.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(12724..13137)
                     /gene="ibpA"
                     /locus_tag="OHN28_002875"
     CDS             complement(12724..13137)
                     /gene="ibpA"
                     /locus_tag="OHN28_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCQ2298241.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(13131..13203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            13443..13775
                     /locus_tag="OHN28_002876"
     CDS             13443..13775
                     /locus_tag="OHN28_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2298242.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(13779..14993)
                     /locus_tag="OHN28_002877"
     CDS             complement(13779..14993)
                     /locus_tag="OHN28_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCQ2298243.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            15094..16194
                     /gene="cbrA"
                     /locus_tag="OHN28_002878"
     CDS             15094..16194
                     /gene="cbrA"
                     /locus_tag="OHN28_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCQ2298244.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(16155..17492)
                     /locus_tag="OHN28_002879"
     CDS             complement(16155..17492)
                     /locus_tag="OHN28_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298245.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17567..18715)
                     /gene="dgoD"
                     /locus_tag="OHN28_002880"
     CDS             complement(17567..18715)
                     /gene="dgoD"
                     /locus_tag="OHN28_002880"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCQ2298246.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(18712..19329)
                     /gene="dgoA"
                     /locus_tag="OHN28_002881"
     CDS             complement(18712..19329)
                     /gene="dgoA"
                     /locus_tag="OHN28_002881"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCQ2298247.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(19313..20191)
                     /gene="dgoK"
                     /locus_tag="OHN28_002882"
     CDS             complement(19313..20191)
                     /gene="dgoK"
                     /locus_tag="OHN28_002882"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCQ2298248.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            complement(20188..20877)
                     /gene="dgoR"
                     /locus_tag="OHN28_002883"
     CDS             complement(20188..20877)
                     /gene="dgoR"
                     /locus_tag="OHN28_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCQ2298249.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
BASE COUNT         5236 a         5560 c         5214 g         4963 t
ORIGIN      
        1 atccctgcgt gatattgtct gtaaaaaagg agcagatatg cacactcacc ctcacttaat
       61 aaaacaaact cttgttccac gactcattaa tgaaataaac aaaagaacac aaagcaatag
      121 tgagaagtta tcaaacagtg ataaattaac atatattaat gacaactact gtgagataac
      181 acgaaactac acgtcatttt atggcatgaa cattctgagc atcgttcttt ttttacctat
      241 cgttatttta tgttttggct ctgttttatt ctttatatat gacattatat ttaacttcga
      301 acgatactta gatacgtgga gataccgcgc agactctgag ttactgctac atattgctta
      361 ctgcgcaatt tttttcatgt cttttgtttt tgctgctatc ctaaattatt ttatctttgc
      421 cccgcgacat tacccaattc gttttaatag aaaaacagga aaggtctata tttgtaatta
      481 tattcttaat gatatgagtc ataaatcgac ctataccttc tggtggcact acccattcta
      541 caaacatact aagcctgaat ataaagagta cgactgggaa caaattcaag gtatagcttt
      601 ttttgcgcaa aatcaccatt ccacatacgc caccattcgc tgtattgttt atgaatcatt
      661 agacagtaca aatattattg acacctttaa cctgataacg acagagagtg ctacaaatgc
      721 attgcatatg acaaataaca aattatggtt atggattaat agctttatga attttgacga
      781 tgagttttta gagaaatcta tcaacactgc tccaggtatt tggggccgtg aagtgaattg
      841 gcctgatgat atgaataaga aatcaatgtt gtcttcaatg gacgaatata tactctgaat
      901 aatttgagtt gcagaatata agctcgcccg gatgtaaata tcccatccag gcgagaaaat
      961 cacttactga aaaaacaccg caatcttgtt aaacatcgtc gggtcggact gattacgctg
     1021 cactttcacc acatcttcca gcttgtcgat ttggcttatc atctgctcca gacgctggtc
     1081 gtcattgacc agtagccaga tgtggctttt gtcgctgtcc tgaatcggca ggcagagaat
     1141 gccttcaacg ttaaaagcgc ggcgggcaaa aaggccacaa acgtgggtca ttacacccgg
     1201 atgattgcga acggtgagct ccagaatcac gttatcatga gttgtgtttt gcatggctta
     1261 ttcccccacc atttcagtat tcgccgcacc tggcggcacc atcgggtaca ctttttcttc
     1321 ggcatcaatg cgcacatgga tcagcgccgg gccagggcga ttgatgattt cctgcaatgc
     1381 agcctgcgga tcggcttcgt tattcaaatc acaggtttcg aggccgaatc cggcggcaat
     1441 ctgcataaag ttgattttgc ccggataggt agcggcaaaa acgccctgct tgtagaacag
     1501 actctgttgc tgatgcacca gccccagcgc ttcgttgttc atcaaaatga ttttaacatc
     1561 cagctgattt tcactggcgg tcgccatctc ctgaatattc atcatcaggc tgccgtcgcc
     1621 ggagaagcac aacactttgc ggtccgggtt cgccagcgcc gcgccaatcg ccgcaggcag
     1681 gccaaaaccc atcgtgccca gcccgccgga ggtcagccac tggcgtgggc gattgagcgg
     1741 ataggcttgc gcggtccaca tctgatgctg acccacgtcg gtagtgataa tcgcattgtc
     1801 atcgacacag gcggcaacgg cgttgatcag gccgtaatgg cttaatggat cgcacgcttt
     1861 cgggattgga cacgggaatt cacgctgcaa atccgccacc aactggtgcc actctgcacg
     1921 cggttgcgct tccaccagcg ggatcaactg cgccagcacg tcatcaacat ccgcctgaat
     1981 tgccacatgc ggttgcttga ttttgcccag ctctgcgcgg tcgatatcga catgaataat
     2041 tttggcattc ggacagaact gctctgtttt gccaatcgcc cggtcatcaa aacgcgcacc
     2101 gagtacaatc agcagatccg cctcctgcaa aatatagttg gtgctgcgca caccgtgcat
     2161 ccccagcata cccagcgaca gcggatgcgc ttttggcaac atacccagcg ccattaaagt
     2221 catggtggta ggcagttgcg ctttctccgc cagttcacgc actcgcgcag gcgcgttgat
     2281 cacaccacca cccagataaa gcaccgggtg tttggcggcg ttaatcatcg ccgccgcgtc
     2341 acgaatactt tcttcgctaa aggcgggtgc ggcggctttt tctgccatag cgggctgtgt
     2401 ttcaatctca aaaactgccg tttgcacatc cttaggaatg tctatccaca ccgggccagg
     2461 gcggcctgat tgcgcaatgc tgaaggcatc gctcatgacc tgcgggagtt cttcgatatg
     2521 tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt cgacttcctg
     2581 gaaggcgtcg gtgccgatca tcgaggcgga aacctgacca gtgatgcaaa tcagcgggat
     2641 ggagtccagc ctcgcatcgg caatggcggt caccaggtta gtcgcacccg gtccgctaca
     2701 ggccatacag accgccggtt taccgtcggt gcgcgccatt ccctgggcga taaaccccgc
     2761 cccttgttcg tggcgagcca gaatatggcg gatttgcgtg ctttggctta aggcatcgta
     2821 aacaggcagg atagaaccgc ccggaatacc cgtcacaatc ttaatgccct gctgttccag
     2881 gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg tgcccgaact
     2941 tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta aacgaaaaac
     3001 cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc cctacggcgc
     3061 attgccgacg accaccacca cacgcacgac gaccactgcg gcggatggcg cagttggtag
     3121 tagttttgcg ttgaacatgg aagtagtcat tagggacctt gttggttttg tgtttaacaa
     3181 tatttataca agcacagctt tacaggggag acaatggaaa atttttcttc cggataaaaa
     3241 tacgtggcag atcacatttt gaactgaaac tcaggcaata aagcatcatc acctgaaaga
     3301 cggtcaggtt gttgacagag tgcgccttat tcatgtcgaa tacagcgtaa acaccttact
     3361 ctaccaaccg caccaccact gtttccggca acatttattg ccgcttttgt ttacatattc
     3421 tgccgctaaa caattcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
     3481 attcctctcc cgcatttatg ggaatgcgta gtgacttatt ctaattattt ttataaaagc
     3541 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
     3601 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
     3661 tctcaatgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
     3721 tgtctcacag ctgttttatg gcccgatttc cgaccgcgtg ggtcgccgac cggtgatcct
     3781 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtctcgacct ccagtttgat
     3841 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
     3901 gcgtactttg ccgcgcgatt tatatgaacg gacacagttg cgccacgcta acagcctgtt
     3961 aaacatgggg attcttgtca gtccgctgct cgcaccgcta atcggtggtc tgctggatac
     4021 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgccg gtgtgacttt
     4081 cagtatggcc cgctggatgc cggaaacgcg tccggtcgac gcaccgcgca cgcgcctgct
     4141 taccagttat aaaacgcttt tcggtaacag cggttttaac tgctatttgc tgatgctgat
     4201 tggcggtctg gccggaattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
     4261 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcggcatt
     4321 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tcaacgttga tgtggcagtc
     4381 ggttatctgt tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
     4441 gaatgtctgg acgctgctca ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
     4501 tccactggct accagcggcg cgatggagcc gttccccttc ctggcgggca ccgctggcgc
     4561 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
     4621 gccgcaaacc ggtcagggca gcctgggatt gttgatgacc ttaatgggat tgttgatcgt
     4681 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcctg tttaagctca
     4741 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
     4801 atcacccgca tatgcggcgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
     4861 ttgtctggtt tatcttcccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
     4921 tcacggcgcg gttgcgactt ttcgctgcca caccattttt gtaaggtggt gtcaaaaagt
     4981 tcaatcgaaa tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
     5041 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
     5101 agatggggaa taagctgtac tgagcggatg cccagctgtt tgaaattgtg ataaaaatcg
     5161 gcaggttgca gagcatcctt atactctaca accagtttat gcctgtcctc ttcagcatca
     5221 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
     5281 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
     5341 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
     5401 agtgccagag gatcagcgcc acaatggcga ggatgccaat cgaaatccag aataacatgc
     5461 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
     5521 tacgaaacca cgccagtgat gtgcgttccg gctgcagccc tgggtcggca atgcgtctgg
     5581 ctttgcggct atccggcata caacaccagt cccatgacga tcactgccac gaccatcaat
     5641 attaagctga tgatgaataa gctgttggta tagggcaggt cctctttcag gcgcatcgct
     5701 ttttcattgc gtaaccagcg caagtagcca tacatcgcta acccgccgga aaacagacac
     5761 aacaacaacg ccagcagttc gcggatgacg ggcgtggcga aatctggcgc aagttgatca
     5821 agcccaaccc ccgccgccag aaatcctagt gcagtacgga tccacgccag aaaagtacgc
     5881 tcattagcca gcgagaagcg gtaatcgggc gcttctccga ggcgggaaat tttcattgtt
     5941 gctccttgta aactgttcct cgccgaaaaa ttaggccagg aacagcaata atgcatcaga
     6001 acttctgttt tttctcttcc accttcaccc cttgtgtcgg taaaccggtg tcatagtcgc
     6061 gtaccactgg agaataacca tcctgtgggc gtggacgaaa cgcccccatc cagcgcggtt
     6121 gtgcatcttt gcgccacgga cgcaggttcc attgataact gcggaacgga tcgcgaattt
     6181 tgtccatgta atccaataag gcgtcatgca ttttgctgcg aacgtcggca aaatggatat
     6241 catcgatcag gttatgcatt tcatttgggt cattacggcg atcgtaaagt tcatcactgg
     6301 tgaagaggtt gagcaccagt ttaaagtcat ccgttaccca gcaacgcacc ggaataaaac
     6361 cgccaaagct gtcatgctca atctcgtagc gattaaattc caccaacacg ccacgtggtt
     6421 cttttacggc aaggatattt tcccccggca gaatctctgg tttttcaata tctgccagcg
     6481 ccatcattgt cggcagtaag tcaatatgac tgactggcgt atcgacctgt cgccgctccc
     6541 cttgcggcga acggatgatc agcggaatgc gggtgatgtc gtcatacatc gccgctcctt
     6601 tactgatcag cttatgtgcg cccatcattt cgccgtgatc ggaggtataa ataacccacg
     6661 tattttcacg ttgctctggc gttaaggcgt tgatgacccg tccgatttgg tcatcaacaa
     6721 agtcattgca ggcaaaatag agcggatggt gataaagccc gtcatcaccg actggcgatg
     6781 gcatcgcctg cgcccataag cggtgatgtt ccggtttgtt cgccaggtca tcctctgctt
     6841 tctcacccag ttcgtagtaa aaatcagtgt atttctctaa atactccacc ggacaggtga
     6901 acgggtgatg cggctcatca tacgaaatca ccatcaggaa aggttcgtcg gcgcgcgcgg
     6961 gctgttgcag aaaatccacc gcccgattgc tgatgcggtg cgcccaggtg aaggtttcgt
     7021 caatatggtt cgcctgtaaa tcttcgacgc tgtttaggcc attgcgccac aggctaatct
     7081 ctttttccgt cagttcacta agatagttcg ccccatcgaa ccagtaatca gcgtcccact
     7141 ccggcggaca ctcgccaggt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg
     7201 taacaggtgt gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct
     7261 ggcgcgacgt tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg
     7321 gcgcgtgcag gcgtacaaac cggtgaacag gtgtaggcgg agttaaagcg aataccttcc
     7381 gccgccagac tatcaatatt ttgcgtattc agcggcttac cgctatagca accgaccata
     7441 ttggtggcct gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct
     7501 ttgcatcggg agtaaaggtt acggctgggt cgtgacgatg ccgccagctg tcaaaaatca
     7561 ggtagacaat cactacggcg gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt
     7621 agccgccgaa ttcagccagc ccggcataga cgccaatcat cgcgaacagg ataccaattg
     7681 acgcagtctt gacgtttttc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga
     7741 acggcgtggc gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga
     7801 acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga
     7861 tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccgga atgcgcggga
     7921 agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaataccg ttgagctgtt
     7981 tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgcgacc atcaccgaaa
     8041 cgatggcgat aaagaaacca aatttgcgcc cgacggtgac cagctgttgc ggctcggcat
     8101 tctggttaat gatacgccgg taaataccca tactgaataa cgtactggcg ctattcagaa
     8161 agccgttgaa ggtactgatc accgcaccaa ataacaccgc accgaagaag cccaccagtg
     8221 gcaccggcag aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtaaatcct
     8281 gatacagatg aaacgcaatc aaccccggca gcaccagtac cagcgggtcg agcattttca
     8341 gcaccgccgt taacagtgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc
     8401 gttgcacgat gccctgattg gtacaccagt aaaaggtgtt aaccagaatc aaaccggtaa
     8461 atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac tgagtttaat ttctcggcgt
     8521 gaacggtggt aagttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc
     8581 caaataccgg caccatcaac ccgccaataa ccagcccaat accattgatg gagtccgcca
     8641 ctgccattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa
     8701 taaccagtaa ccagatagcc gcaccatcgg aaagttgtag cgactcaccc acatgaaaca
     8761 ggctgttgag cgccaacgcg ccggaataga gaacaatcgg cagaaagcag acgccggtgg
     8821 caatgaggaa gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa
     8881 aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga
     8941 agatcagcgt cactgcagag gtgacttccc agcccatcac cgacatgccg cttttgtagg
     9001 cctgcccgga aaggccgacc agttgttccg tggaaaggtt ggttagcatt aacgaagcgg
     9061 caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat
     9121 ctgttttacg gaccttccac caggtgatga ccgccaccag cagcgtaaaa ccgacaaaac
     9181 tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg
     9241 aatataggga gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc
     9301 gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg
     9361 atattaaaat gaatggaaaa ttgcaaagtt cggatgtaaa aaacgaaact ccgtacaata
     9421 ttcccttatt gattaacgaa aatgtgatct ccagcggaat ttctctgata tcgctctggc
     9481 atacctacgc cgacgagcat tatcgggtga tctggccgcg ggacaagaag aaaccgctta
     9541 tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga
     9601 atgacgaaca aataactctg catggcaact gtattatatt tttaaagcca atggatattc
     9661 actcttatca ctgtgaaggt ttggtctggg aacagtactg gatggaattt acccccacca
     9721 gtatgatgga tattcccgtc ggtcagcaaa gcgttattta taatggcgaa gtttataatc
     9781 aggaacttac cgaagttgct gagttaatta cttcaccaga agcaataaaa aataatctgg
     9841 cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgcac gcagtcggta
     9901 aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctac
     9961 aacaacgctg gagcgtagct gatatggctg ccacgatccc ctgtagcgag gcctggttgc
    10021 gtcgtctgtt tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc
    10081 tggatctggc gctatcgcta ttaaagcaac aagggaactc ggttggcgaa gtcgctgata
    10141 cgctcaactt cttcgactcc tttcatttca gcaaagcctt taagcataaa tttggttatg
    10201 cgccgtcagc tgtgctgaag aatacggaca ggtaataacc agaatggcga gtgtgtaaat
    10261 gactcgatga tgttcagaaa taccaggcgg agtaccattt acggcgactc caccgggaaa
    10321 agagttaacc gatactccag aagagcaccg ccagtaattg gggtgtgata atgcgcagga
    10381 acatcactaa cggatagaca gtggcgtaag agagcgccgc tgcaccgctg gttggatgaa
    10441 gattattagc aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca
    10501 tggtcaggta attcattttg gctaacatcc gcgccagaat accaacagta atcagcggaa
    10561 cggcggtgat cagggcacca taaccaatcc agcttagccc ttcgccatcc accagtgtat
    10621 ggataaaatc gccgccggat ttcaggccaa cgaccgagag gaacagtacg atccccagct
    10681 cccgcagcgc gaggttggcg cttggcggca taaaccagta cagcttgccg atactgccga
    10741 tacgcccgag aatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg
    10801 ccggaaatcc cggcacaaag acgggaatag aacctaacaa tacgccaagc ccgataccaa
    10861 taaacactgg cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg
    10921 caacggcatc aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc
    10981 tgatatcgct acttgcaact aattcaacac cagcacggtt caggcgcgag ataacaacgt
    11041 catagcgttc tttaaagtgc aggtcgcgaa tacgtttacc gagcacgttt tcattggtga
    11101 ccaccacacg ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct
    11161 gaccaatcac cagttgcgca ttatgtaaat ccgcaggctg ccccaccaga tgcagcaaat
    11221 cgcccagttg aataacggtt tctggcgaag gcacttttag ggtttcttct cgtttcaatc
    11281 gtgagcaaat aactttatcg ccattgagaa tcggcacatc tttaatggct aaattatgca
    11341 aattaggatt ctcaactcgg atgttaatcg tcctgattag cgcaccgccg ttggttcgcg
    11401 tggactcgtg ctgctgggcc tcagtctcaa cattgacgcg gaaaataacc cgcagcatcc
    11461 acatagtgaa caaaatgccg caaatcccaa atggatacgc catcgcatag ctcatcccca
    11521 tttgatcgac catcgccatc ggtgtcccca ggtcgcgcaa gatctgctgc cctgccccca
    11581 gcgctggcgt attggtgacc gcgccggaaa aaatccccag cacgaccggc agtggaatat
    11641 caaacagttt atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa
    11701 acaggttgag gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa
    11761 tcccgatggt ataaacgaac aggatcaggc caaattcctg aataacatgc agcatatcgc
    11821 tacttaatgt catccccgcc tgagaaacaa aatggccgac gatgatcccg ccgaacagca
    11881 cgccgccaat gcccaatcca acaccgcgaa acttgacgtt gccgataaac aacccgacga
    11941 ccgccaccaa agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt
    12001 gaaataacat tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata
    12061 gcgcacatta tcacgggaaa gaacgtgccg aaatatctta aacaggcgaa atttaacgca
    12121 ttgttgaaga aaaaagacaa aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
    12181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
    12241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaggttg cgccagagac
    12301 ttccatattt tcagccagcg taaagctcag gctaaaaggc tgattcataa gcccttgatg
    12361 cagccatttt ttctcttctt ttggctgctc cggtgtgcct tttacgctca ggcgcgtacc
    12421 ttccagttga atctctaaat cttcctgacg aaaacctgcc agcgcgaggg taatgcggta
    12481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctggcttt caccggcgtt
    12541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
    12601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
    12661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttagg
    12721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
    12781 gatatacagc agaccattca ccaggttagc accacgaaca tgaatgttct cagctaactg
    12841 gaatttacgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
    12901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
    12961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
    13021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
    13081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
    13141 caatagctcc tgaaatcagc gagaatctaa gaccttccac aatggacagg tcaggtagcc
    13201 agaacaccca tcaggcgcgt cctcatcggc tacgatgtaa aaatgggtct ggaaatgaac
    13261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
    13321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg cgacgtaatg atgatgttat
    13381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
    13441 ttatgataaa aaatgtgttg ttagcgttca tgatatgcag cggaatgaca ttactcggag
    13501 gatgctccag cgtgatgtcc cataccgggg ggaaagaagg aacgtatcct ggcacccgcg
    13561 ccagcgcaac catgattggc gacgatgaaa ccaactgggg cacaaaatcg ctggcgatac
    13621 tcgacatgcc atttaccgca gtactggaca caatattgct accgtgggat gtgttccgca
    13681 aagacagctc agtgcgctcg cgcgttgaaa aaagcgaagc cgacgcgcaa gaaaccaata
    13741 acgtcattcc ccccgccaaa atgcctgcaa actgagagct agcggtcagt ttcggtgatc
    13801 cacaactgac cgtcctccat ccacgccaac cattgaccat ccggcgagaa aaccacggcg
    13861 tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca
    13921 tgggcacaag caatccgatt atccagcaca aatcccagcc attcgcctga tggatgccag
    13981 ttaaatgcag actgaatatc cgttttatta tgggttaact ggcgtggttc accgccctgt
    14041 ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc
    14101 gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaatcc cggagaagcc
    14161 cgatggtgcg taaaggttaa acgtcgctgc acgacaccac gcggtggcgc gggcagggtt
    14221 gtttccgttc cacttaacgg cgcatcacct gccactttcc agccagcttc atcttgcggc
    14281 aactcgacaa taaacagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata
    14341 aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcactg
    14401 ccaggctgtg gcgtgggcgt ggttttactt accagcacgc accagtggct accgctgtat
    14461 tcacgcggat gctgcttttg tacgttgacc gggccaaacg gtgcagcaac accgacattt
    14521 cgcaaatcca gcgccggatc gagttcatgc attacatggt cgttgtaggt aaagctcacc
    14581 ctttcaccgt ttgggctaaa gacatgtaca tgactgccgc cgcgcagcgc tcctggcgta
    14641 tatggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgctttc aacaatcact
    14701 ccgcgccgat gatgaaagtc gtaataccat gtttcatcag gattttccgg gccgtggatg
    14761 aagacatact tctctgactt cggatgaacg gtcaccacgc cgacgtatgc gccctgtgac
    14821 gcgcgataga taacctcgac ctcgccggta tggatattca cacgctcaat ggtctcgccg
    14881 gtaaacgacg ccccagaagg ccgcacgtca aacaccagcc actggctgtc gggcgtccag
    14941 gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg
    15001 tcctgataca aaacggttcc cgccatcata cttcacaagg acttcacttt cagacaatag
    15061 tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt
    15121 ggcctcggcc cggcagggtc ggcgttggcg cgaaagttag caggcaaaat gcaggtgatc
    15181 gcgctggata aaaagcacca gtgtggtact gaaggtttca gcaaaccttg tggcggtctt
    15241 ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtc
    15301 atcgccaacc cgcagatttt cagcgtcaaa accgtcgacg tcgccgcatc gctcacgcgt
    15361 aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg
    15421 ctgatccccg ccagcgttga ggtttaccac gacagcctgt gtcgaaaaat ctggcgtgag
    15481 gatgataaat ggcatgtcat ttttcgcgca gacggctggg agcagcatat taccgcccgc
    15541 tatctggtcg gtgccgatgg tgccaactcg atggtgcggc gacatctcta cccggatcat
    15601 cagattcgta aatatgtcgc catccagcag tggttcgcag agaaacatcc ggtaccgttc
    15661 tactcctgca tctttgataa tgcgataact gactgttact catggagtat cagcaaagac
    15721 ggttatttta tctttggcgg tgcctatccc atgaaagacg gtcagacgcg tttcacgacg
    15781 ctaaaagaga aaatgagcgc ctttcagttc cagtttggta aggcggtgaa aagcgaaaaa
    15841 tgcacggtgc tgtttccctc acgctggcag gattttgtct gcggcaagga caacgccttt
    15901 ctgattggcg aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg
    15961 ctggatagcg cagagattct gcgttcggta ttactgaagc agccagagaa gctcaatacc
    16021 gcttactggc gcgccactcg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc
    16081 cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggagt ggcacatatt
    16141 ccacagttga aagattatcc aacgcgcttc acatcgccca ccagcaggat gtaagagagc
    16201 gcgccaatca acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc
    16261 gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc aaagttaaac
    16321 acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggtt
    16381 atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga
    16441 tcgttggtgt agttcgcacc cataatgcag gtggagatca gcaagccgca gataatcggc
    16501 gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca
    16561 gagagcagga cgccaataaa cgccgcgagg aatggcaccg tggtcataaa acctgctttc
    16621 agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac
    16681 agtgtagaag ccaccgcaaa ttgcccaaga tagacaccga tcagtttacg atggaacacc
    16741 agtttccagt ctttggctgt taacggctga cgcgcctctt ttttcaccgg cgcatcgcca
    16801 tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat gcctttggtc
    16861 agacgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccaccg
    16921 gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc
    16981 agaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcaga agcgcgttca
    17041 tgttccggga accaactggt taccatccgg ttattggtcg ggaacgcggg cgcttcgaaa
    17101 ataccggtta tcgcgcgcag accaattaat gacattaagc ccgtggcaaa gccctggaat
    17161 aacgtcgcca ctgaccagcc aaatatcgca ataaaataag tcacgcgtga acctacgcga
    17221 tctaaaaacc aaccgccggg gatctgacat agcgtataaa gccaggcgaa ggccgaaaat
    17281 acatagccca tttgcgcttt agtaatacca aactcttcct gaatatgggc agaagccacg
    17341 gccaggttag cacggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc
    17401 agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc
    17461 ctgattttgg gcatagcgaa gccgctcacc atgcactgta atttacagag ggttatattt
    17521 ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta
    17581 cgctgttatc ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact
    17641 caataacttt agcttcgtcg atttccacgc ctaagcccgg tttcgttaat ggtttaaaga
    17701 aaccgccgac catgctgaag tcctctttgt ttttcacaaa gtcgagtaac tccgcgcctt
    17761 tgttgtaatg aatgcccata ctttgttcct gaaggacggc gttataggaa acaaagtcga
    17821 tatgcaggca agccgccagt gcaatcggtc cgagcggaca gtgcggcgca agggtcacgt
    17881 cataggcttc tgccattccg gcaattttgt agcattcggt aataccgcct gcgtgggaga
    17941 gatccggttg cagaatcgaa ataccgcctg cctccagcac acgtttaaaa tcgaagcgtg
    18001 agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg tgccgccagt ttcgggtagt
    18061 attcggcctg ttccgccagc accggctctt caataaacag cggacgatac ggttccagct
    18121 ctttaatcag cactttcgcc atcggcgcgc tgacgcgacc atggaagtcc agaccaaact
    18181 caatctgatt gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg
    18241 cgcgggagtt atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc
    18301 cgatttcgcg tagcgtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc
    18361 aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccag accggcgcat
    18421 tcagcacttt gcctttgata tcccataaag cctggtcgat cccggcgatg gcactcatca
    18481 gaatcggacc gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc
    18541 gtgacgggtc ctgaccaatc aaatagtcac tcagctcgtg gactgccgct tccaccgtgc
    18601 gagcgcgacc ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt
    18661 tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac
    18721 tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcagtccgtt ctacggactg
    18781 cccggcgcga tagagatcgc tacctaagcc cgctccggca cagcctgcat ctatccactg
    18841 cgccaggttt tctggcgtca caccgccaac ggcaaagacc gcgatgttcg gtggtaatac
    18901 cgcttttaat gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagtgc
    18961 ctgcgcaccc gcgtcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcagac
    19021 ggtcatgccg tagcccaccg cacggcggat cacttcacta tggatattgg gcgtaacgat
    19081 gagctgacag cccattctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc
    19141 gccaatcaac gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg
    19201 tggagaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg
    19261 cgccagcgcc tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg
    19321 ccactgcatg agcgatgttc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg
    19381 tcacgtcaca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg
    19441 cgacaagggt gatgacttgt tgatgggcca catagtcgcg catactggcg acctctgcgc
    19501 caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat
    19561 gcgaggcgcg aacttcaaaa agctgtggca atacggcggg cgcattaagg ccacgctcaa
    19621 gaccagccgc gaaagcatcg acagcgtttt cctgcggcgg caaacctgcg ccaatcaatg
    19681 agtgatttaa cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttaattt
    19741 gctggctatc ggcctgcacc catttgcaat gcgtaccggg catgacataa agagaggaag
    19801 gagcgagagt gcgcgcgccg atcaattgtg tttcttcgcc acgcatcaca ttgtggttat
    19861 cgtcatgaga gacgcataat ccgggaataa tccagatatt gtcgccaaca gacgttaatt
    19921 gttcgccaat agacgaaaaa cgggcaggaa tggataaata cggcgcaact ttccagccga
    19981 cattgctgcc gaccattcct gccattacca ctggcgtatt ctcttcacgc cagtcggtcg
    20041 tcacttctgc taacaccgca gccggagatt ttccattcag gcgcgtgacg cctgcttctg
    20101 attgcctgct gtccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc
    20161 cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct
    20221 ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc ttatcgcatc
    20281 gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt
    20341 tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctcagttgtt gtaacaccgg
    20401 gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc
    20461 gcggtcctga ttgttagcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct
    20521 ggaggtcgcg cgctctgctg cccaacgggc aatagccggc tctaccaggt ttcgcacttc
    20581 gctcattgca ctgataagcc gtgggtcata gtcgttttcc agcacccatt gcagtacgtc
    20641 agtgtcgagg taattccact ggttacgcgg agccacaaac gccccgcgat aacgtttcat
    20701 ttcaatcagc cgcttcgcca tcagcgaacg aaatacctca cggatgatgt tgcgcgaggt
    20761 tgcaaactcc tcacagagtt ctgcctcagc cggaagcggc gagcctggca cgtatttgcc
    20821 gtgaacgatc tgtttaccca gcgtaataac gatgcgatcg gttttattga gagtcatgga
    20881 gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa
    20941 ttttgtagta ccagcgtgat gacgttcgcg ttt
//
LOCUS       DAJOTB010000080        20720 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJOTB010000080 DAJOTB010000000
VERSION     DAJOTB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.080"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(158..742)
                     /gene="pth"
                     /locus_tag="OHN28_002884"
     CDS             complement(158..742)
                     /gene="pth"
                     /locus_tag="OHN28_002884"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2298250.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1020..1298
                     /gene="ychH"
                     /locus_tag="OHN28_002885"
     CDS             1020..1298
                     /gene="ychH"
                     /locus_tag="OHN28_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCQ2298251.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1353..3032)
                     /gene="dauA"
                     /locus_tag="OHN28_002886"
     CDS             complement(1353..3032)
                     /gene="dauA"
                     /locus_tag="OHN28_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCQ2298252.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3157..4104)
                     /gene="prs"
                     /locus_tag="OHN28_002887"
     CDS             complement(3157..4104)
                     /gene="prs"
                     /locus_tag="OHN28_002887"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCQ2298253.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4255..5106)
                     /gene="ispE"
                     /locus_tag="OHN28_002888"
     CDS             complement(4255..5106)
                     /gene="ispE"
                     /locus_tag="OHN28_002888"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCQ2298254.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5106..5729)
                     /gene="lolB"
                     /locus_tag="OHN28_002889"
     CDS             complement(5106..5729)
                     /gene="lolB"
                     /locus_tag="OHN28_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCQ2298255.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            5943..7199
                     /gene="hemA"
                     /locus_tag="OHN28_002890"
     CDS             5943..7199
                     /gene="hemA"
                     /locus_tag="OHN28_002890"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCQ2298256.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7241..8323
                     /gene="prfA"
                     /locus_tag="OHN28_002891"
     CDS             7241..8323
                     /gene="prfA"
                     /locus_tag="OHN28_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCQ2298257.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8323..9156
                     /gene="prmC"
                     /locus_tag="OHN28_002892"
     CDS             8323..9156
                     /gene="prmC"
                     /locus_tag="OHN28_002892"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2298258.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9153..9545
                     /locus_tag="OHN28_002893"
     CDS             9153..9545
                     /locus_tag="OHN28_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCQ2298259.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9549..10358
                     /locus_tag="OHN28_002894"
     CDS             9549..10358
                     /locus_tag="OHN28_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCQ2298260.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            10394..11248
                     /gene="kdsA"
                     /locus_tag="OHN28_002895"
     CDS             10394..11248
                     /gene="kdsA"
                     /locus_tag="OHN28_002895"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCQ2298261.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(11397..11504)
                     /locus_tag="OHN28_002896"
     CDS             complement(11397..11504)
                     /locus_tag="OHN28_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2298262.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(11909..13009)
                     /gene="chaA"
                     /locus_tag="OHN28_002897"
     CDS             complement(11909..13009)
                     /gene="chaA"
                     /locus_tag="OHN28_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCQ2298263.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            13279..13518
                     /gene="chaB"
                     /locus_tag="OHN28_002898"
     CDS             13279..13518
                     /gene="chaB"
                     /locus_tag="OHN28_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCQ2298264.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            13667..14362
                     /gene="chaC"
                     /locus_tag="OHN28_002899"
     CDS             13667..14362
                     /gene="chaC"
                     /locus_tag="OHN28_002899"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2298265.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14406..14759)
                     /gene="ychN"
                     /locus_tag="OHN28_002900"
     CDS             complement(14406..14759)
                     /gene="ychN"
                     /locus_tag="OHN28_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCQ2298266.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            14944..16338
                     /gene="ychO"
                     /locus_tag="OHN28_002901"
     CDS             14944..16338
                     /gene="ychO"
                     /locus_tag="OHN28_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCQ2298267.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(16339..16989)
                     /gene="narL"
                     /locus_tag="OHN28_002902"
     CDS             complement(16339..16989)
                     /gene="narL"
                     /locus_tag="OHN28_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCQ2298268.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(16982..18778)
                     /gene="narX"
                     /locus_tag="OHN28_002903"
     CDS             complement(16982..18778)
                     /gene="narX"
                     /locus_tag="OHN28_002903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCQ2298269.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            19117..20508
                     /gene="narK"
                     /locus_tag="OHN28_002904"
     CDS             19117..20508
                     /gene="narK"
                     /locus_tag="OHN28_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCQ2298270.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5125 a         5158 c         5373 g         5064 t
ORIGIN      
        1 ccgacgttgg gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata
       61 tcttaataat caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt
      121 atacacggca ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca
      181 atcggttcgt tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt
      241 cgtcgatggc ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac
      301 cgacaacttt atttttatcg cccggatgac cgattccgat gcgtaaacgg tgaaagttag
      361 ggttattacc caatttactg atgatgtctt tcagtccatt gtgaccacca tgaccgctac
      421 ccaacttaaa tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt
      481 cgtccggatt aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca
      541 taaatgtagt cgggactaac aggcggacat cttcgcctcc aagggtgact cgcgaagtat
      601 aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa tcgctctgcc agtaagtcaa
      661 cgaaccaggc accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc
      721 cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag
      781 tttactggtt gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aatgcaatta
      841 ttgcctaagt ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt
      901 gaaataaggg ttgtaattgt gatcacaccc gcacataacc cacagggtgt tgtctatact
      961 ttacacataa ggaagagggg tattccctgt tacaacccag aaagttccgg aggtgacata
     1021 tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa
     1081 tggtggtcgg cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt
     1141 atttcgcaca tggtgctgtg ctaagtattt tcgtcggtgc gattctctgg ctggcgggtg
     1201 cccgtgttgg cgggcatgaa caggtgtgcg accgttactg gtgggttcgc cactatgaca
     1261 aacgttgccg ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc
     1321 agtcagtttg cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga
     1381 ttcgggaaga atgcgaggcg tcccgggatc ggttgaatgc ctgcgcgagc catagtgcgc
     1441 agtggctgga attccaggtt gcacacgcgc agttcacatc cttcgggcag acgcttcaca
     1501 aaacgctgga acgcatcaag accaccagca tcaagtaccg gaacggcatc ccacttcaga
     1561 atcacaatcc gtttgccttc aagacgtgac tccaggtccg tgaataagcc ttcagcggca
     1621 gcaaaaaaca gcgggccaat aacgcgcaga accaggacat cgtctggaac gtctacgact
     1681 accggtgcca ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg
     1741 atccccacgc tgatggcaat aaccatatcg aacagcacgg tcagcgacat gcacagcagc
     1801 atgacgatga tgtcatcttt cggtgcatga cgcagcaagt cgaccacttt gtgcgcttca
     1861 ctcatgttcc acgccaccat caacagcagg gctgccatgg cggaaagtgg cagccaggag
     1921 agcagcggtg ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgccgag
     1981 ataggggaag ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg
     2041 gtaatgccac caaagaacgg agcgataata ttccccagtc cctgtccaac cagttcgctg
     2101 ttcgccttgt gtttcgtccc ggtcatacca tccagcacca cggcgcagag cagagattcg
     2161 attgcgccga gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag
     2221 gttagcgtga attctgaatt aggcagatcc cacggcagca ccagttgcgg cagcagttgc
     2281 ggaataccgt taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta
     2341 gcaacatgtc cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc
     2401 ggaaggtggc cgggtaaacg tatgcccaga cgcggccaga aaaccagaat gccaagcgtc
     2461 acaatgccaa tggctgcatc acccacatta atggtcggca gcgccataaa taatgcgccg
     2521 actttttgta gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc
     2581 tgcatggtac cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga
     2641 atatactcaa tcaggcgacc aaagcgtgcc agccccatca gaatcaaaaa gatccccgac
     2701 agcaaggtcg caaccagcag tcctgccagt ccaaactgtt gcgaaacggg atagagaatt
     2761 accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg accctcccgt cagagcaatg
     2821 acaatccccg caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata
     2881 gccaacgcca tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca
     2941 cgggtaaacc gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa
     3001 ggcatcacat gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat
     3061 ctgtcaacta aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa
     3121 aaaacccgcc gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagatat
     3181 cgattcttcg ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt
     3241 acgcacgttc ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac
     3301 ttcatcaatt acagagttac gcaggttgtt cgccgcgttg ccagagaaga tcgggtgagt
     3361 cgcgtacgca aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag
     3421 cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc
     3481 gatgatatgc atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat
     3541 atcggtatcg ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg
     3601 agaaaccaca attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct
     3661 accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc
     3721 cactgtcagc acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt
     3781 gattggtaca cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat
     3841 aacagcggtg atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc
     3901 catcaggttg tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg
     3961 tacattttca ttaatttgta cactgacttc gccgtcgcta aagcgaccta cagcggcgtc
     4021 gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc
     4081 agcaaaaagc ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag
     4141 aatctgccga aaactgtgcg ggccaggcat gattctttcc aggcggtgta ttaaagagcg
     4201 cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag
     4261 catggctctg tgcaatgggg aaagattaac gcctttcgcc acaaagccat tgagccattc
     4321 cggggcttgc tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca
     4381 ggcccctgtc ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc
     4441 aacctcgcga aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag
     4501 caacgtttct attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac
     4561 cggtgtcgga atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac
     4621 cggcgttagt atttcaccaa cgccttcggc aaacgcggca tgaccacgaa caaagacagg
     4681 aacatctgcg cccagcgtca gccccatttc cgccagctca tccatgctta gtccgcattg
     4741 ccagagatga tttaacgcca ccagaaccgt cgcggcattg gatgaaccac cgccgagacc
     4801 gccgcccatc ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcgaaagacg
     4861 cccgctgtct gccgcagttt tcatcaacaa ccgcgctgcg cgaacgatca ggttatcttc
     4921 atgttccacg ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac gaagctcaat
     4981 gctgatgatg tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc
     5041 cgcacgctga ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt
     5101 ccgcattatt tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg
     5161 agttccatat tggctggcat cgcaggttga gtttttgtgt cataaccacc gtaaacaact
     5221 ttccagtttt tgccattctg gctgtaggtg atttcgctca ggcggtactg gtcgtccagt
     5281 ttgtaatcgg tcgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga
     5341 atcggcattc cggttagttt accaatcatc tcttcggcgt catcggcggt gtaacgctga
     5401 cctttattgt cgactaattg tacgttaccc ggctgagcat tcagctccag ttccgtgctg
     5461 cccaatgggt tagttaacag cagacgatag cgatcctggc cggtttgttg ccagaagaag
     5521 cgagcgtaca ctttttgttg gtcggaaata taagcgaatg caccgcgagt ctgatactga
     5581 ttaagattac gcacgtcttg ctggtgctga cgccactgtg gcgaatccgg gcttttgcct
     5641 gggcctttgg gtgtggtaac agaacaggca gtgagcacga gggcagctag cggtagcagg
     5701 cggataaagc gaaaatcggg cagggtcata ataatgacaa gtccttgaga tacgttgcag
     5761 ttataaccct taatgctagc gttaccgtta gccatcgtct atgttcacgt tgtcttaatt
     5821 gccagaatct aacggctttc ggcaattact ccaaaagggg gcgctctctt ttattgatct
     5881 tacgcatcct gtatgatgca agcagactaa ccctatcaac gttggtatta tttcccgcag
     5941 acatgaccct tttagcactc ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac
     6001 gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga cagtctgctc gcgcagccga
     6061 tggtgcaggg cggcgtggtg ctgtcgacgt gtaaccgcac agaactttat ctcagcgttg
     6121 aagaacggga tgacctgcaa gaggcgctca ttcgctggtt gtgtgattac cataatctca
     6181 acgaagacga tctgcgtaat agcctctact ggcatcagga taacgatgcg gtcagtcatt
     6241 taatgcgcgt tgccagtggg cttgattctc tcgtccttgg cgagccgcag atcctcggtc
     6301 aggttaaaaa agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc gaactggaac
     6361 gcatgtttca gaaatctttc tctgtggcga aacgcgttcg cactgaaaca gatatcggtg
     6421 ccagtgctgt gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc
     6481 tctctacggt gacagtactg ctggtgggtg cgggcgaaac catcgagctg gtggcgcgtc
     6541 atctgcgcga acacaaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc
     6601 aaattctggc agatgaagtc ggcgcggaaa ttattgccct gagtgatatc gacgaacgtc
     6661 tgcgcgaagc cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag
     6721 gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg
     6781 ctgttccgcg cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagcg
     6841 tggacgatct gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc gcagcggttg
     6901 aggcggaaac tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgtgcacaaa
     6961 gcgccagcga aactattcgc gagtatcgca gccaggcaga gcacgttcgc gatgagttaa
     7021 ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc acaagccatt atgcaggatc
     7081 tggcatggaa actgactaac cgcttgatcc atgcgccaac aaaatcactt caacaggccg
     7141 cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc
     7201 agtacatcat tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc
     7261 caaactggaa gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca
     7321 aactatcgcc gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga
     7381 tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat
     7441 gatgcttgac gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga
     7501 aaaaagcgag caactggaac agcaattaca ggttctgtta ctaccaaaag atcctgatga
     7561 cgaacgtaac gccttcctcg aagtccgtgc cggaaccggc ggcgacgaag cggcgctgtt
     7621 cgctggcgat ctgttccgta tgtacagccg ttacgccgaa gcccgccgct ggcgtgtaga
     7681 aatcatgagc gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag
     7741 cggtgatggt gtatatggtc gtttgaaatt cgaatctggc ggtcatcgcg tgcagcgtgt
     7801 tcctgctacg gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc
     7861 agaactgcct gatgcagaac tgccggacat caacccagca gatttgcgca tagatacttt
     7921 ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac
     7981 tcacttgccg accgggattg ttgttgaatg tcaggacgaa cgttcacaac ataaaaacaa
     8041 agctaaagca ctttctgtac tcggtgctcg catccacgct gctgaaatgg caaaacggca
     8101 acaggccgaa gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa
     8161 ccgtacttac aacttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta
     8221 ccgcctggat gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga
     8281 acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg
     8341 ttacgtgaag caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc
     8401 ctgctggagc atgttaccgg caaagggcgt acttttattc tcgcctttgg tgaaacgcag
     8461 ctgactgacg aacaatgtca gcaacttgat gcgctactga cgcgtcgtcg cgatggtgaa
     8521 cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca
     8581 gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg agcaggcact ggcgcggttg
     8641 cctgaacaac cttgccgtat tctcgatctc ggaacgggta ccggggcgat tgcgcttgcg
     8701 cttgccagcg agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc cgatgctgtc
     8761 gccctggcac aacgtaatgc ccagcatctg gctatcaaaa atatccacat tctgcaaagc
     8821 gactggttta gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat
     8881 attgacgagc aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg
     8941 ctggttgcgg cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac
     9001 gcgctggtat ccggcggctt tctgcttctg gagcatggct ggcagcaggg tgaagcggtg
     9061 cgacaggcat ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga ctatggggat
     9121 aacgagcgcg taacgctcgg ccgctattat caatgacaag tttttctacg ttgcttagcg
     9181 ttcatctcat tagtatcgcg ctttctgttg ggctattgac cttacgtttc tggctacgtt
     9241 atcagaagca tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata
     9301 cgctgttatt gttaagcggc attgcgttga tggctaaagc gcacatccag ccattttccg
     9361 ggcaggcaca gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt
     9421 ttattgcact cgattatcgt cggatgcaca gtcagcaggc gcgcattatt gccttcccgc
     9481 tggcgttggt ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg
     9541 ggtaagtcat gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca
     9601 tgatcctggc ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac
     9661 tggagcgtct cgttagtctg gcgaaggaag aaattagcca gcttctgcct ttagaagagc
     9721 agttggaaaa actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg
     9781 tttatcgtct ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg
     9841 cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc
     9901 cggtgatttt ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc
     9961 tgattaatcc ttttaacggt gaatcgttaa gcgaacatat gctggatgtg tggttaaagg
    10021 gaaatatcag cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg
    10081 aggtaatccg caaattgctg gatacactca aagcttcgtt gatggaagaa aatcagatgg
    10141 agctggcgtt acgcaccagc gaagctttat tacaattcaa cccggaagat ccctatgaaa
    10201 ttcgcgatcg cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt
    10261 taagttattt cgttgaacag tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa
    10321 taaataacat cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaaga
    10381 ttaaggcgat cctatgaaac aaaaagtggt tagcattggt gacatcaacg tagcaaatga
    10441 cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg
    10501 catttgcgag cactacgtaa ccgtgaccca gaaactgggt atcccttacg tgttcaaagc
    10561 ctcttttgac aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga
    10621 agggatgaaa atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt
    10681 tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc
    10741 gtttcttgct cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa
    10801 cgtcaagaaa ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa
    10861 agaaggcggc aacgaaaaag tgattctttg cgatcgcggt gctaacttcg gctatgacaa
    10921 cctggttgtc gatatgctgg gcttcagcat catgaagaaa gtgtctggta attcgccggt
    10981 gattttcgac gttacccacg cactgcaatg tcgcgatcca tttggcgcag cttccggcgg
    11041 tcgccgtgct caggtggctg agctggcaag agccggtatg gcggtaggtc tggcggggct
    11101 gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc
    11161 gctggctaaa ctggaaccgt tcctcaagca aatgaaagcg attgatgatc tggtgaaagg
    11221 tttcgaagaa ctggatacca gcaagtaatc ttttttgcct gaaaaataaa gtcttagcgt
    11281 tctgcgttaa gacttttttc atgggtgccg gatacaaaaa aggccgcagg ctgttacccc
    11341 tgcggccggt ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac
    11401 ttccggttac gccaccagct gacaatcact gcggtaataa tccccgccag gatcggtgcc
    11461 gccaggttgt gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt
    11521 aatgacaaca ttttgcggct attcttgaat tgttctggtt caagattagc ccccgttctg
    11581 ttgtcaggtt gtacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
    11641 gggataaagc ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta
    11701 cgtcgcaatt catcttcact tcatccctga aatgtttgca ataaagagta cattccggtt
    11761 tttcaacagc tgttgcagtc atttcgtgca ggttgtggta caggcttgca attctgatta
    11821 acaacctgtc cagctagtat gaagaaatat aataagcagg aatttatatt atctcccccg
    11881 atttccgtca tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa
    11941 acagtgccag atgcgctgcg ccattgagca cgttagttcg tccggtggag aaggagatat
    12001 ggcacagtac taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgca
    12061 attcgttacc cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaatggttg
    12121 ccaacactga accaaagaac agattcatcg cgcgctggac ctggttattc aacactgctt
    12181 ttaacgcacc taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg
    12241 cgacaggcgc attcatgctg tcgagcaatg tctccagcgg gctggcgttc attttggtga
    12301 ctgcaataac ggcaatcaga tggacaatca accagatggc atgccacagg ctgctatggg
    12361 cagacggttt accgtggtgc gggtcgtcat catcactgtc atcttcgtgc tcgtagacaa
    12421 acaaactttg atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa
    12481 ttaatgctac cagtaacgcc tgacctgttg aaaaattcgc cgcaggcaga gccattggaa
    12541 ataccagtac gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca
    12601 gattcatata ttgggtggca aacttacgtc cgcccaacaa taatgaaaag ccaaccagcc
    12661 cacctgtaac aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcat
    12721 cgccggttgc cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa
    12781 gaataagcga accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa
    12841 cactaaaggc gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg
    12901 gtagtgtctg gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa
    12961 gcgaagtctc cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc
    13021 ctttgcagat gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaattgtc
    13081 tggatatcgg taacatttta cgaattttta ccccgctgtc ggttttttac tcatttgggc
    13141 ataaaaataa gtaatacgtt tagacaatgt ttgttttagc acttcttaag aagagtctga
    13201 catgaaaatt cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact
    13261 ttgtaaaagg agtcaacgat gtcgtataaa acgaaaagcg atctgccgga aagcgtaaag
    13321 cacgtgcttc cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat
    13381 caatataaag ataaagaaga tcggcgtgat gacgccagtc gcgaagaaac agcgcataaa
    13441 gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa
    13501 aaatcgtcaa accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc
    13561 cgtggattgc atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga
    13621 caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga
    13681 tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg
    13741 gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg
    13801 accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga
    13861 gtcgtgcacc ggtacactgg ttggatggca tcgcgcgttt tgcctgcgtc tgaccgccgg
    13921 gcgcgggact gcgcaccagc cgggacgaat gcttgcactg aaagagggcg gacgcaccac
    13981 aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc tgctgtggaa
    14041 gcgtgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg
    14101 acgcacagta aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga
    14161 cactcgcgct caggtcatag cgccgttgat tgccgcggcg agcggtccgc tgggaaccaa
    14221 tgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg
    14281 gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt
    14341 gttacggccg ggattcgcct gagtaaactt ccctcatagt ggggcgtccg acgccccctc
    14401 aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag ttcaacaaga
    14461 gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag
    14521 gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg
    14581 ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg
    14641 aacagacgca gatccagatt gttctcctgc tctcgtaacg caatggccag ccgcaagctg
    14701 ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt
    14761 atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt
    14821 ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag
    14881 gtgccagtaa tcgttattct ttaaggctat ggtttttcat ttttaccgga agttaccgac
    14941 gttttgagcc gtttcgttcc tcgcattatt ccgttttatt tactcttgct ggcggcaggc
    15001 ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatcactt tgagaatagc
    15061 aatgatggtc tgccggattt aggcatggca cctgaacatc atgatgggga aaaacacttt
    15121 gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc
    15181 gagcaggcaa aagctttcgc attgggaaaa gtccgcgatg cgcttagtca acaggttaat
    15241 cagcacgtag agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt caaagtggat
    15301 aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca agataatgat
    15361 cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggct ggtgagcaat
    15421 gtgggcgttg ggcaacgctg ggcgcgcggc agctggctgg tgggttataa cactttttat
    15481 gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gcgctgaagc gtggggcgaa
    15541 tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga acagactgcc
    15601 acgcaggaac aacggatggc gcgcgggtac gacctgacag cccggatgcg catgccgttc
    15661 tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgtcgatttg
    15721 tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc
    15781 cctgtgccct tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa
    15841 aataacctcg ggctgaatct taactaccgc tttggtgtac cgctcaaaaa acaactttct
    15901 gcgggcgagg ttgtcgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga
    15961 aataatcttc cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca
    16021 ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt
    16081 tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc
    16141 gcacaagcca acagtgagga gggctggacg ctgatcatgc ctgactggca gaacggggaa
    16201 ggggcgagca atcactggcg attgtctgta gtggtggaag ataaccaggg gcagcgtgtc
    16261 tcctccaatg agatcacgct aacgcttgtc gaaccgttcg acgcattgtc aaacgacgaa
    16321 ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg cagcttccac
    16381 gcgagacttt agcttcattt tcttcagcat gtgcttgacg tgaactttta ctgtgctttc
    16441 ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag
    16501 aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc
    16561 gcgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc
    16621 tgcctgatgt aacgctttca gcagatcttc cggttccata tcttttaaca gatagccatc
    16681 cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac
    16741 aatgcgcccg gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc
    16801 gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca gttcaatacc
    16861 ctgttcgcca ttactcgctt cgccaaccac agtgatatct ggtgccatac tgataagctg
    16921 ttttacgcca gttcgcaaca tcgggtgatc gtcgatcagc aggatagtag ccggttcctg
    16981 attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca
    17041 ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg
    17101 cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc
    17161 cgcagccgtt atcctggacg gtcagtttga cctgattatc gttttgcgcc accgtcacca
    17221 cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca
    17281 acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca
    17341 ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc
    17401 caggctcggt gagctgcaaa cggaatgtgg tgagcaattc acgcaactgc acccaggatg
    17461 cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc
    17521 cctgcatctg taagcaactc acctgcatct tcatgcaaga gagagattgg gcaatagaat
    17581 catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt
    17641 cctgatggcg atccagcgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt
    17701 gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact
    17761 gcgtatgagt gtcagccagc cgccacttca gggttgtgcc gcgatcgcca acgggtaata
    17821 cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctgac tggcaggtaa
    17881 actcctgatg attctcttca tcatcagtgt catacacccg cagttcgata tcacgtagca
    17941 gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcgcggg
    18001 aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta tgctccagcc
    18061 cagcggtttt ctcctgaacc cgctgctcaa gtacggcata actttccgcc agttctgcag
    18121 acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc
    18181 gttgggtaaa atcgcgatgg ctgacggcac tcgccattgc cagcagttgc cgccacggtt
    18241 gtagcaatct cgcccgcaac cagataatag tgaataccag taaaagtgcc ataaataccg
    18301 ccattacccg atggaccagc accactgtct caatgcgcat ttccgtggtg cgatcaaaac
    18361 cagataccag ctgatcaagc ccggcaacaa actggctgac atccgctgac accgtttcgc
    18421 ggttttgtgc acgtatcagt gcagggatca gttcattacg ccagtaatct tgtaaaccct
    18481 gtaattgcgc cagttgcccg tctcgttctg ctgctcgagt caactcggcg ctaaatgctg
    18541 tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact gctgccaaca
    18601 gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa
    18661 cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca
    18721 acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt
    18781 aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct
    18841 ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa gagttactcc
    18901 ttatttgccg tgtggttagt cgctttacat cggtaagggt agggatttta cagtaccgtg
    18961 aaaaatctca taatttttat gaaatcactg tactcactat ggataatgat aaatatcaat
    19021 gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct acagacacta
    19081 aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc cgcccccgaa
    19141 agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt ctggcaacaa
    19201 cgcggtcaac gtattgccag ccgcaacctg tggatttccg ttccctgtct gttgctggcg
    19261 ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt cggttttaat
    19321 tttacgaccg atcagctatt tatgttgact gcgctgcctt cggtttctgg cgcgttatta
    19381 cgtgttccat actcctttat ggttcctatc tttggtggtc gtcgctggac ggcgttcagc
    19441 accggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg
    19501 ccttatagcg tcttcatcat catttctctg ctgtgcggct ttgctggcgc gaacttcgca
    19561 tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt
    19621 ctgaatggtg gtctgggcaa catgggcgtc agcgtcatgc agttggttgc tccgctggtg
    19681 gtatcactgt cgattttcgc agtatttggt agccagggcg tcaaacagcc ggatgggact
    19741 gagctgtatc tggcgaatgc gtcctggata tgggtgccgt tccttgccat cttcaccatt
    19801 gcggcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa ggagcagttg
    19861 ccggtactca aacggggtca tctgtggatt atgagccttc tgtatctggc aaccttcggc
    19921 tccttcatcg gcttctccgc gggctttgcg atgctgtcaa aaacgcagtt cccggatgtt
    19981 cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg ttctgcaggt
    20041 ggtgcattat ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt tatcctgatg
    20101 gcgattttca gcggcctgct gttcctgacc ttaccgactg acggacaggg cggaagcttc
    20161 atggcgttct tcgcagtctt ccttgcgctg ttcctgaccg ctgggctggg tagtggttcc
    20221 actttccaga tgatttccgt gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa
    20281 gggggttctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc ggcgctgggt
    20341 ttcatctctg cgattggcgc gattggtggc ttctttatcc cgaaagcgtt tggtagctcg
    20401 ctggcattaa cgggttcgcc agtcggcgca atgaaagtat ttttgatttt ctatatcgcc
    20461 tgcgtggtga ttacctgggc ggtatatggt cggcattcta aaaaataaat cgtaatattt
    20521 gattatcctt tgcgcggcat gatgtcgcgc tttttttatg cgtcatttag ttacaacata
    20581 ctaatgttgt atggcttatt tcgctggatt tcattaagag tcattaatat gttacccatg
    20641 gggaatactc cttaataccc atctgcataa aaatcttaat ggtttaaata accacaggtg
    20701 taaaacgtct taatttacag
//
LOCUS       DAJOTB010000081        20718 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJOTB010000081 DAJOTB010000000
VERSION     DAJOTB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.081"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(108..737)
                     /locus_tag="OHN28_002905"
     CDS             complement(108..737)
                     /locus_tag="OHN28_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCQ2298271.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(727..1731)
                     /locus_tag="OHN28_002906"
     CDS             complement(727..1731)
                     /locus_tag="OHN28_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCQ2298272.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(1755..2522)
                     /locus_tag="OHN28_002907"
     CDS             complement(1755..2522)
                     /locus_tag="OHN28_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCQ2298273.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            2739..4166
                     /gene="rmuC"
                     /locus_tag="OHN28_002908"
     CDS             2739..4166
                     /gene="rmuC"
                     /locus_tag="OHN28_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCQ2298274.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            4261..5016
                     /gene="ubiE"
                     /locus_tag="OHN28_002909"
     CDS             4261..5016
                     /gene="ubiE"
                     /locus_tag="OHN28_002909"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCQ2298275.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            5030..5635
                     /gene="ubiJ"
                     /locus_tag="OHN28_002910"
     CDS             5030..5635
                     /gene="ubiJ"
                     /locus_tag="OHN28_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCQ2298276.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5632..7272
                     /gene="ubiB"
                     /locus_tag="OHN28_002911"
     CDS             5632..7272
                     /gene="ubiB"
                     /locus_tag="OHN28_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCQ2298277.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            7351..7620
                     /gene="tatA"
                     /locus_tag="OHN28_002912"
     CDS             7351..7620
                     /gene="tatA"
                     /locus_tag="OHN28_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCQ2298278.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7624..8139
                     /gene="tatB"
                     /locus_tag="OHN28_002913"
     CDS             7624..8139
                     /gene="tatB"
                     /locus_tag="OHN28_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCQ2298279.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            8142..8918
                     /gene="tatC"
                     /locus_tag="OHN28_002914"
     CDS             8142..8918
                     /gene="tatC"
                     /locus_tag="OHN28_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCQ2298280.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8960..9742
                     /gene="tatD"
                     /locus_tag="OHN28_002915"
     CDS             8960..9742
                     /gene="tatD"
                     /locus_tag="OHN28_002915"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCQ2298281.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9739..10227)
                     /gene="rfaH"
                     /locus_tag="OHN28_002916"
     CDS             complement(9739..10227)
                     /gene="rfaH"
                     /locus_tag="OHN28_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCQ2298282.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            10394..11887
                     /gene="ubiD"
                     /locus_tag="OHN28_002917"
     CDS             10394..11887
                     /gene="ubiD"
                     /locus_tag="OHN28_002917"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCQ2298283.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11933..12634
                     /gene="fre"
                     /locus_tag="OHN28_002918"
     CDS             11933..12634
                     /gene="fre"
                     /locus_tag="OHN28_002918"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCQ2298284.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12815..13978)
                     /gene="fadA"
                     /locus_tag="OHN28_002919"
     CDS             complement(12815..13978)
                     /gene="fadA"
                     /locus_tag="OHN28_002919"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCQ2298285.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13988..16177)
                     /gene="fadB"
                     /locus_tag="OHN28_002920"
     CDS             complement(13988..16177)
                     /gene="fadB"
                     /locus_tag="OHN28_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCQ2298286.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            16367..17698
                     /gene="pepQ"
                     /locus_tag="OHN28_002921"
     CDS             16367..17698
                     /gene="pepQ"
                     /locus_tag="OHN28_002921"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCQ2298287.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17698..18312
                     /locus_tag="OHN28_002922"
     CDS             17698..18312
                     /locus_tag="OHN28_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCQ2298288.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            18351..19802
                     /gene="trkH"
                     /locus_tag="OHN28_002923"
     CDS             18351..19802
                     /gene="trkH"
                     /locus_tag="OHN28_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCQ2298289.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19814..20359
                     /gene="hemG"
                     /locus_tag="OHN28_002924"
     CDS             19814..20359
                     /gene="hemG"
                     /locus_tag="OHN28_002924"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCQ2298290.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         4821 a         5242 c         5663 g         4992 t
ORIGIN      
        1 tgtacaggcg caatgctgag ggcagcgcct gcaagaagta ccattgcagc catttttttc
       61 ggtaaggtaa gatgttttat cattgctttt atatccttat ttatcagtta gttgctattt
      121 aaattgttcc atgcgttcat aaacagtact gcattttcat agacttgctc tggcgtcatg
      181 ccggggtgat atagcgcaga accgaggcca aagccgtttg cgccgtggct cagataacgc
      241 gccatggttt ccggtgtgat accgccaacc ggtaacatcg gaacggattg tggaataacc
      301 gcacgccagg ctttggtaac ttctggcgta atttgttcag ccgggaacag cttgatggca
      361 ttagcaccat gttcaatggc agtaaaggct tcggtgggcg tggcgacgcc aggcgcacaa
      421 tacataccca gcgcacgggc gcgacggatc accgcagtat cgcaatgcgg catcacgata
      481 atctggcccc cagcctcttt aaccgcaacc acctgctcta ccgaaagcac agttccggct
      541 ccgaccaggc agtccttagg cagggcgtcg cgcagtaaac tgatactttg caacgcctcc
      601 ggcgagttca taggaatctc aatcaggcgg aatccggcgg cgtacagttt ttcaccgatg
      661 gcgacaatct catccggttt cacgccgcgt aaaatggcga ctaacccggt tttttgcaaa
      721 agcgtattaa atggcatggg tacattccct ggcgttggtg gacagtaacc cggcagcgtg
      781 ggcgattcgc cataatccct gttcagtggc attaggaatg gcgtgtatct gtctgtcggg
      841 aaaaacgtgg ctgaatgctt gtgaatagcg gctgttcaga tttgcgctac cgattaaggc
      901 gatcggtgtt gtggcgggta aatcacgtag caaactggat gccagaccac ataattcatg
      961 gcctatcagt aatccggaaa ggtaatcccc ctgcgaggac gacgtaagat tactggtcag
     1021 caggcgcgaa cgggtcgaaa acagcgtcga taataatccg gtctgatgat gctcttgtgc
     1081 tgctgtcagc ccgtgggtga aggcatccca gtgcagttca tctccaggat gcatggtggc
     1141 accgagaatc gaatggcggg ataaaacatc aaataactcg cccgtcataa aggttcggaa
     1201 atcggtaatg gtgttgtttt caactactgc ccatttcgca tgtgtgccgg gtaagcctat
     1261 cagcactggc ataccctgat ggatcgcatt ttgcagggac ggttccatcg agatagcacc
     1321 gaagatttgt gtctcttcgc cacgcatcac ttcgggcatt tcaccttctt taatcacgcc
     1381 aggaatgatg tgcagcattg tctgctgctg gtggataaca ggcgtcaact gcttcgccag
     1441 accctcgaga gtgaccgggc aaggtaaata agctgctggt tgccaccctt gcgcactgcc
     1501 gagcatgccg caagccagta ccgggcatgg ggtttgtgcc agccagtcgc cgcagacacg
     1561 taaaaacgta tcgtggaaag cgtcctcacg tgttgctgcc tgggagggta atttcatgat
     1621 cccccagggg aattcacgtt tttcttgtgg atttggtgat tcgccaaaac gccatgcccg
     1681 aagcgatgac gttccccagt ccagggcaat aaatgagggt gctcccggca ttagtcgttt
     1741 cctttaatca tggtttattt atctttgcgg acgtgcttac cccagcccag ttcagcggat
     1801 atctcttctg cacaggattt gacgtaaggt gccagttctt ccagacgcgc cagcgacata
     1861 tacgtggtgg tactggcgac agaaatagcc gccacaattt catcccgggc gttgtaaacc
     1921 ggcgctgcaa cgcaacggat agtcggctcg ttctcttcca gatcaaaagc aaaaccactg
     1981 gcggcataac gacgcatgtt ctgaataaat gcgcccagtt ttgtttcatc accacaggtc
     2041 ttaaacagcg tgcgccactc ttcttctgtt cggttgagga tcaatgcctt gccgatgccg
     2101 gtgattgcca gtggcatgcg atggcctggg cgtgagcgca tttccagccc gcgctgactg
     2161 ttgatcttct ccagatacag cacttcgtcg ccttcaacga tccccagatg cacggtgtcc
     2221 agggtgtaac gggcaaggcg ttgcaaaacg ggctgggcaa tttcgtacag cgaggtgctt
     2281 tccagtgctc tggcaccgaa ttcgatcagt ttggcaccca gttggtagcc ctgcgttggg
     2341 acatggcgca ggtagcgttg ttccaccaga acggtgacta aacgatgggt tgtgctccgg
     2401 gtcgtgccgg tgaactcgcc gatacttttt aggtcatggc agccgttata aacagcatta
     2461 agaaccgcca gtccacgtaa taacgtttgt gcgcctgcgg tggttgaaga ggtttctgcc
     2521 atacgtttct cctgatgata ttgctgaagt gttccacata atgacgtggg gaaaaggtga
     2581 tatatcccac aatataaact tcatctcata ttatgggata aaataatgaa aattaacgat
     2641 cgttttgcgt agcgcctcgc aggaaacttc tttcaaactg gacgtttgta cagcacaatt
     2701 ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt
     2761 acgcagtcat tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg
     2821 cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg
     2881 caaaacaaca aattacccaa agcgagcact ggcgcgcaga gtgtgaattg ctcaataacg
     2941 aagtgcgcag cctgcaaagt attaacacct cgctggaggc cgatctgcgt gaagtgacca
     3001 cgcggatgga agccgcacag caacacgctg acgataaaat tcgccagatg atcaacagcg
     3061 aacagcgcct cagtgagcaa tttgaaaacc ttgccaaccg tatttttgaa cacagtaatc
     3121 gtcgggttga tgagcaaaat cgccagagtc tgaacagtct tttgtcgccg ttgcgtgaac
     3181 aactggacgg ttttcgccgt caggttcagg acagtttcgg taaagaagca caagaacgtc
     3241 ataccctgac tcacgaaatc cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag
     3301 cgatcaacct gacgcgtgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgaag
     3361 tggtgctaac ccgcgtactg gaggcgtctg gtctgcgtga agggtatgaa tatcagactc
     3421 aagtgagtat cgaaaccgac actcgttcgc gaatgcaacc ggatgtcatc gtgcggctgc
     3481 cgcagggcaa agatgtggtg atcgacgcca aaatgacgct ggttgcctat gaacgttatt
     3541 tcaacgctga agatgactac acacgtgaaa gcgcgctaca ggaacatatc gcgtcggtgc
     3601 gtaaccatat ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc
     3661 tggattacgt gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgtc
     3721 agccggagct gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc
     3781 tgctggtggc gctgcgcact atcgccaacc tgtggcgtta tgagcatcaa agccgcaacg
     3841 cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg
     3901 acatgtccgc gattggtcaa agtctcgaca aagcgcagga taactatcgc caggcaatga
     3961 aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag
     4021 gggtagaaat taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg
     4081 aagagtatcg acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg
     4141 atgaatataa tcagcagtcg cgctagccca ttgggggtag ttaagccggg tagaaatcta
     4201 gggcatcgac gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag
     4261 atggtggata agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa
     4321 aaagcggata tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat
     4381 gatttgatgt cattcggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc
     4441 gtacgccgtg ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa
     4501 ttctctcgcc tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg
     4561 ctcaaaatgg gccgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt
     4621 caggcgaacg ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg
     4681 tttggtctgc gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg
     4741 aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc
     4801 aaagcctatg atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac
     4861 gacgccgaca gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc
     4921 ctgaaaacca tgatgcagga tgccggattc gaaagtgttg actactacaa tctgacggca
     4981 ggggttgtgg cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa
     5041 acctttagtg acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc
     5101 gctgaaaacg gcccgctcgc gtctgttggg caaagtgctg cgcgtggagg taaaaggctt
     5161 ttcgacgtca ttgattctgg tgttcagcga acgccaggtc gatgtactgg gcgaatgggc
     5221 aggcgatgct gactgcaccg ttatcgctta cgccagcgtg ttgccgaaac ttcgcgatcg
     5281 ccagcagctt gccgcactga ttcgtagtgg tgagctggaa gtgcagggcg atattcaggt
     5341 ggtgcaaaac ttcgttgcgc tggcagatct ggctgagttc gaccctgcgg aactgctggc
     5401 cccttatacc ggtgatatcg ccgctgaagg tatcagcaaa gccctgcgtg ggggcgcaaa
     5461 gttcctgcat cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga
     5521 gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt
     5581 cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca
     5641 ggtgaagtac ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa
     5701 ctgatcccca aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg
     5761 atgccaaatc ggcataaaga caaaccttta ggtgagcgac tacgactggc cctgcaagaa
     5821 ctgggaccag tatggatcaa gttcgggcag atgttatcaa cccgccgcga tctttttccg
     5881 ccgcatattg ccgatcagct ggcgttattg caggacaaag tcgccccgtt tgatggcaag
     5941 ctggcgaagc agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac
     6001 gattttgaaa tcaagccgct ggcttctgct tctatcgccc aggttcatac cgcgcgattg
     6061 aaatcgaatg gtaaagaggt ggtgattaaa gttatccgcc cggatatttt gccggtcatt
     6121 aaagcggatc tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat
     6181 ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa
     6241 ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc gacgcaattt tgaagacagc
     6301 ccgatgctct acatcccgga agtttaccct gactattgta gtgaagggat gatggtgatg
     6361 gagcgtattt acggcattcc ggtgtctgat gttgcgacgc tggagaaaaa cggcaccaac
     6421 atgaaattgc tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc
     6481 tttttccatg ctgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac
     6541 ccgaaatata tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc
     6601 tatctggcgg aaaactttat cgccttcttt aatcgcgact atcgcaaagt ggcagagcta
     6661 cacgtcgatt ctggttgggt gccaccagat accaacgttg aagagttcga atttgccatt
     6721 cgtacggtct gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta
     6781 ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg
     6841 ttactccaga aaaccctgct ctacgtcgaa ggggtaggac gccagcttta tccgcaactc
     6901 gatttatgga aaacggcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt
     6961 cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa
     7021 ctgcctgaac tggtttacga cagtttgcgc cagggcaagt atttacagca tagtgttgat
     7081 aagattgccc gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc
     7141 ggaattggcg ctacgttagt attaagtggc acattcttgt tggtcagccg acctgaatgg
     7201 gggctgatgc ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc
     7261 aaaacacgct gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt
     7321 ttaatcatca tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg
     7381 attattgccg tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc
     7441 gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag
     7501 gataaaacca gccaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat
     7561 acgaatcagg aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa
     7621 tctgtgtttg atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt
     7681 ctggggccgc aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgtgcgttg
     7741 cgttcactgg cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt
     7801 caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa
     7861 gcgtcgatgg atgaattacg ccaggctgcg gagtcgatga agcgttccta cgttgcaaac
     7921 gatcctgaaa aggcgagcga tgaagcgcac accatccata acccggtggt gaaagacaat
     7981 gaaactgtgc atgaaggcgt aacgcctgct gctgcacaaa cgcaggccag ttcgccggaa
     8041 cagaagccag aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa
     8101 accgctgcac cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc
     8161 aaccgcttat cacgcatctg attgagctgc gtaagcgtct gctgaactgc attatctcgg
     8221 tgatcgtgat attcctgtgt ctggtctatt tcgccaatga catctatcac ctggtatccg
     8281 cgccattgat caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc
     8341 cgttctttac gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga
     8401 ttctctatca ggtgtgggcg tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg
     8461 tggtgccgct gctggtttcc agctctctgc tgttttatat cggcatggcg ttcgcctact
     8521 ttgtggtctt tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtacagg
     8581 tatccaccga catcgcgagc tatttaagct tcgttatggc gctgtttatg gcgtttggtg
     8641 tctcctttga agtgccggtg gcaattgtgc tgctgtgctg gatggggatt acctcgccag
     8701 aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgttgc
     8761 tgacgccgcc ggatgtcttc tcgcaaacgc tgttggcgat cccgatgtac tgcctgtttg
     8821 aaatcggtgt cttcttctca cgcttttacg ttggtaaagg gcgaaaccgg gaagaggaaa
     8881 acgacgctga agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg
     8941 ttgtcatatg gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc
     9001 gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat
     9061 caccggtacc aatctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc
     9121 ctgttggtca acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgtgactga
     9181 agaagcgatt attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct
     9241 cgactttaac cgcaactttt cgacgccgga agagcaggaa cgcgcttttg ttgcccagct
     9301 acgcattgcc gcagaattaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg
     9361 gtttatgaca ttgctggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt
     9421 taccggcaca cgcgaagaga tgcaggcgtg cgtggcgtgt ggaatttata tcggcattac
     9481 cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc cgttgattcc
     9541 ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg ctccctcgcg atctcacgcc
     9601 aaagccatca tcccggcgca acgcgccagc ccatctgccc catattttgc aacgtattgc
     9661 gcactggcgt ggagaagatg ccgcatggct ggctgccacc acggatgcca atgtcaaaac
     9721 actgtttggg attgcgtttt agagtttgcg gaactcggtg ttctttacgc tgtgcttaat
     9781 ttctttattg ataagattaa gcaatagcat ggagcgagcc tctccgtctg gctcggtgaa
     9841 aatggcctga aaaccttcga atgcgccttc agtaataatc actttatcac caggatacgg
     9901 cgtcgatgga tcgacaatat ctttcggttt atacactgat aactgatgaa tcaccgccga
     9961 tgggactatc gctggcgacg cgccaaagcg cacgaagtgg ctgacaccgc gggtcgcgtt
    10021 gatagtcgtg gtatgaatca cttccggatc gaactccaca aacaagtagt tggggaacaa
    10081 tggctcactg actgcagtac gtttgccacg cacgattttt tccagggtga tcatcggtgc
    10141 caggcaattc acagcctgtc tttcgaggtg ttcctgggca cgttgaagtt gcccgcgctt
    10201 gcagtacagt aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg
    10261 atagcaaaag ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt
    10321 tcacgctaat ttaacaaatt tacagcatcg caaagatgaa cgccgtataa tgggcgcaga
    10381 ttaagaggct acaatggacg ccatgaaata taacgattta cgcgacttct tgacgctgct
    10441 tgaacagcag ggtgagctaa aacgtatcac gctcccggtg gatccgcacc tggaaatcac
    10501 tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg ctgttgttcg aaaaccctaa
    10561 aggctactca atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg tggcgatggg
    10621 catggggcag gaagatgttt cggcgctgcg tgaagttggt aaattattgg cgtttctgaa
    10681 agagccggag ccgccaaaag gtttccgcga cctgtttgat aaactgccgc agtttaagca
    10741 agtattgaac atgccgacaa agcgactgcg tggtgcaccc tgccaacaaa aaatcgtctc
    10801 tggcgatgac gtcgatctca atcgcattcc cattatgacc tgctggccgg aagatgccgc
    10861 gccgctgatt acctgggggc tcaccgtaac gcgcggcccg cataaagagc ggcagaatct
    10921 gggcatttat cgccagcagc taattggtaa aaacaaactg attatgcgct ggctgtcgca
    10981 tcgcggcggc gcgttggatt atcaggagtg gtgtgcggcg catccgggcg aacgtttccc
    11041 ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc ggtgcagtca cccccgttcc
    11101 ggatacgctt tcagagtatg cgtttgccgg attgctgcgc ggtaccaaga ccgaagtggt
    11161 gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg gagattgtgc tggaagggta
    11221 tatcgaacaa ggcgaaactg cgccggaagg gccgtatggc gaccataccg gttactataa
    11281 cgaagtcgat agttttccgg tatttaccgt gacgcatatt acccagcgtg aagatgcgat
    11341 ttatcattcc acctataccg ggcgtccgcc agatgaacct gcggtactgg gtgtagcact
    11401 gaacgaagtg ttcgtgccga ttctgcaaaa acagttcccg gaaattgtcg atttttatct
    11461 gccgccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg
    11521 acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa
    11581 atttgtgatc gtttgcgatg atgacgtcaa cgcccgcgac tggaacgatg tgatttgggc
    11641 gattaccacc cgtatggacc cggcgcggga tactgttctg gtagaaaata cgcctattga
    11701 ttatctggat tttgcctcgc ctgtctccgg gctgggttca aaaatggggc tggatgccac
    11761 gaataaatgg ccgggtgaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga
    11821 tgttgtcgcg catattgacg ccatttggga tgaactggct atttttaaca acggtaaaag
    11881 cgcctgatgc gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac
    11941 cttaagctgt aaagtgacct cggtagaagc tatcacggat accgtatatc gtgtccgcat
    12001 cgtgccagac gcggcctttt cttttcgtgc tggtcagtat ttgatggtag tgatggatga
    12061 gcgcgacaaa cgtccgttct caatggcttc gacgccagat gaaaaagggt ttatcgagct
    12121 gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa
    12181 agatcatcaa atcgtggtcg atattcccca cggagaagcg tggctgcgcg atgatgaaga
    12241 gcgtccgatg attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct
    12301 gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga
    12361 gcagcatctg tatgatctct gcgagcttga ggcgctttcg ttgaagcatc ctggtctaca
    12421 agtggtgccg gtggttgaac aaccggaagc gggctggcgt gggcgtactg gcaccgtgtt
    12481 aacggcggta ttgcaggatc atggtacgct ggcagagcat gatatctata ttgccggacg
    12541 ttttgagatg gcgaaaattg cccgtgacct gttttgcagc gagcgtaatg cgcgggaaga
    12601 tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata aaaaacccgc ccctgacagg
    12661 cgggaagaac ggcaactaaa actttataca gtggcattac tctacgttgc atccggcaaa
    12721 cagtgcccga tgcaacgctg ccgcgtctta tcgggcctac aaaagttctg aaccgtgggc
    12781 cggataaggc gttcacgccg catccggcaa gtggttaaac ccgctcaaac accgttgcaa
    12841 tgccctgacc cagaccgata cacatcgtcg ccagaccaaa ctggacgtct ttgtgttcca
    12901 tcagattcag cagcgtggtg ctgatacgcg caccggaaca acccagcgga tgacccagcg
    12961 cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg ctccattagc ccgagatctt
    13021 taatacacgg caggatctgc gcggcaaagg cttcgttcat ctcgaatacg ccgatatcgc
    13081 tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc cggaaccgga ccataaccca
    13141 taatagatgg gtcacaaccc acaaccgcca tcgaacgcac gcgggcgcgc ggcttaagac
    13201 ctaattcacg ggcgcggctt tcactcatca ccagcatggc agctgcgcca tcggaaagcg
    13261 cagaagatgt acccgccgtt accgtaccgc tgacgggatc aaacgccgga cgcagtgtgg
    13321 cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac tgcttcaata
    13381 cgccgtcggc atcgtgacca ccggtcggga tgatttcatt tttaaatgcg cctgactgcg
    13441 tggcggccca cgcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga
    13501 taccgtgcat acgcgccagc atttctgccg ttaagcccat catgcccgcc gctttggcga
    13561 cattacggct caggccgggg tgaaaatcga cgccgtgact cattggcaca tggcccatat
    13621 gctccacgcc gccaaccaga catgcctgcg catcaccggt catgatcatt cgtgctgcgt
    13681 catgcagtgc ctgcatggat gaaccacaca agcgattaac ggtaaccgcc gggacagagt
    13741 gcggcacttc agccaacagt gccgcgttac gggcgatatt aaaaccctgc tccagtgttt
    13801 gctgcacaca accccagtaa atatcgtcaa gggccgccgc ttccagtgcc gggttacgcg
    13861 ccagcaggct acgcattaaa tgagcggaga gatcttccgc acgcacgtta cgaaaagcac
    13921 cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg
    13981 tgactcctta agccgttttc aggtcgccaa ccggacgggc tggctcaacc gggggatagt
    14041 acggttcgtt atgacgtgct ttattacgca gaccttccgg tacttcatac agcgggccga
    14101 ggtgctgata ttgctgtgcc atatcgaggt attttgcgct gccgagggtg tccagccagc
    14161 ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta gaccagcgcc atatccgctt
    14221 ctgccggagt ggcgataatg ccctcttcca gacagcggac cacttcgttg accatcggga
    14281 tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg cttcggctgg ctcacttttg
    14341 ccagcaggtc gtcaacgacg acatcttctt ctttcttcgg cttacctttg ctgtcttctt
    14401 tataacgcca gaaaccgagg ccgttcttct gaccaaagcg gttggcatca aacagcgcgt
    14461 cgatggcatc gcgataatct ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct
    14521 gggcgtgatg cgcggtgtca atgcccacaa catccagcag atatgccgga cccatcggcc
    14581 agccaaactg tttttccatc actttgtcga tcttgcggaa atccgcgccg tcgcgcagca
    14641 actggctgaa accggcgaaa taggggaaca gtacacggtt gacgaagaag ccggggcagt
    14701 cgttaaccac aatcggcgtt ttgcccatct tgctcgccca ggcgacgact ttcgcgatgg
    14761 tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa cggcattcgg tggaccgggt
    14821 taaagaagtg catcccgcag aagttttccg gacgttccag cgcgttggcc agttcgctga
    14881 taggaatggt tgaagtgttc gacgccagta cggtatccgg gcgtactttc tgctcggttt
    14941 ctgccagcac ggcttttttc actttcgggt tttcaacaac cgcttctacc acaacatcca
    15001 cgcggtcaaa cccggcgtag tcgagcgttg ggtgaattgt ggagatcacg ccagccagtt
    15061 tcagaccatc gatcttgccg cgctcaagct gcttgttcag cagtttcgcg gcttcggtca
    15121 tgccgagggt taacgacttg tcgttgatat ctttcatgac aaccggcacg cctttccacg
    15181 cagactggta agcgatgccg ccgcccataa tgcctgcgcc cagcacagcg gcctgtttcg
    15241 gggtttcaac atctttggtg agtttcttcg ctttaccttt tacatattga tcgttaagga
    15301 aaatgccgac cagtgcgcgg gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt
    15361 ccaggtttaa ggcttcttca cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg
    15421 tgatgggggc cggataatgt ttccccgccg tttgtgcgac catcccttta gcgatggtga
    15481 agctcatggt ggcttcaatc ttgctcagtt ttagcggttc cagcttcggc tgacgtttcg
    15541 ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat cgccatcgcc ccttcaatca
    15601 gtttttctgc tttgactaca ccatccacca gaccgatttt cagcgcctga tccgcgccga
    15661 catctttacc ggcggcaatg atttccagcg cactgtcagc gcccagcata cgtggcatac
    15721 gtacagaacc gccaaagccc ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca
    15781 gatccggcgt cgccagacga taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg
    15841 catagccgtt gacggcagca atggtcggca ccgggagatc ttccaggcga ttaaacacgc
    15901 tattggcaaa gtgcagccac tgacttaact gttcttcagg aacgaggaac agggacaaaa
    15961 attcggtgat atcagcaccg acgataaagg ctgctttgtt cgaacgcagc agcagccctt
    16021 ttagatctga ttgctgttcc agcacgccga tggcctcgcc gaggctggcg acggtcgcag
    16081 tgtcgagttt attaactgaa cctggggcat caaataccag ttcggcaatg ccatcttcca
    16141 gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc agtctcctga atccgcaagg
    16201 tgatctggtc gtaccagatg catcgaagtg tgtattttgt gttaaaaata tgcaaataaa
    16261 agattaaaga aatgccgatc tgatcacgct cggcagaaat cacgctctgg atgaacgatg
    16321 tgctaagatg cggagactta aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc
    16381 ctcactctat aaaaatcata tcgctacctt acaagaacgg actcgcgatg cgctggcgcg
    16441 cttcaagctg gatgcgttac ttattcactc cggcgaactg ttcaacgttt ttctcgacga
    16501 tcatccctat ccgtttaaag tgaacccgca attcaaagcg tgggtgccgg taactcaggt
    16561 gccaaactgc tggctgctgg tggatggcgt gaacaagccg aaactgtggt tttatctgcc
    16621 ggttgattac tggcacaacg tcgaaccgct accgacctcc ttctggactg aagatgtaga
    16681 agtgatcgcg ctgccgaaag ccgatggcat tggtagcctg ctgcctgctg cgcgcggcaa
    16741 tatcggttat atcggtccgg tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa
    16801 catcaacccg aaaggggtga tcgactacct gcattactac cgctccttca aaaccgagta
    16861 cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc aatggtcatc gtgcggcaga
    16921 agaagcgttc cgttctggca tgagcgagtt cgatatcaac attgcctatc tgaccgcgac
    16981 cggtcatcgt gataccgacg taccttatag caacattgtg gcgcttaacg aacacgctgc
    17041 ggtgttgcat tacaccaaac tggaccacca ggcaccggaa gagatgcgca gcttcctgct
    17101 ggatgccggg gccgaatata acggctatgc ggctgatctg acccgtacct ggtcggcaaa
    17161 aagcgacaac gactacgcac agctggtgaa agacgtaaat gatgagcaac tggcgctgat
    17221 cgccaccatg aaagcaggcg tcagctatgt ggattaccat atccagttcc atcagcgcat
    17281 cgccaaactg ctgcgtaaac atcaaatcat caccgatatg agtgaagaag cgatggtcga
    17341 aaacgatctc accggaccgt ttatgccgca cggtatcggc catccgctgg gcctgcaggt
    17401 gcatgacgta gccggtttta tgcaggatga tagcggtacg cacctcgcgg caccggcaaa
    17461 atatccgtac ctgcgctgca cccgtattct ccagccgggc atggtgttaa ccatcgaacc
    17521 gggtatctac ttcatcgaat cgctgctggc accgtggcgt gaagggcagt tcagcaaaca
    17581 cttcaactgg cagaaaattg aagcactgaa accgttcggc ggcattcgta tcgaagacaa
    17641 cgtggtgatt cacgaaaaca acgtggaaaa catgacccgg gatctgaaac tggcgtgatg
    17701 gaaagctggt taattcctgc ggcaccggtc acggtcgttg aagagatcaa aaagagccgc
    17761 ttcattacgc tgttggcgca taccgatggc gttgaggcgg caaaagcgtt tgttgaatcg
    17821 gtgcgggcag aacaccccga tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg
    17881 gatgattctc aacagctggg tttctctgac gacggggagc cggcgggaac ggcaggtaaa
    17941 ccgatgctcg cccaactaat gggcagcggc gtcggggaaa ttaccgctgt ggtggtgcgc
    18001 tactacggcg gcatattgct tggcaccggt gggttagtga aagcgtatgg cggcggcgtg
    18061 aatcaggcgc tgcgccagct aacgacccaa cgcaagacac cgttaaccga atatactttg
    18121 caatgtgaat atagtcagtt aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa
    18181 attatcaaca gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa
    18241 gtggctgaat tttccgcaaa gctggcggat tttagccgtg gttcattgca attgttagcg
    18301 attgaagaat aatccccact tcgttttgca gaactaagga agcggcagag atgcattttc
    18361 gcgccattac ccgaatcgtt ggactactgg ttatcttatt ttcagggacc atgattatcc
    18421 ctgggctggt agcactcatc taccgggatg gagcgggccg cgcttttacc cagacctttt
    18481 ttgtcgccct cgccattggc tctatgcttt ggtggccgaa ccgcaaagag aagggtgaac
    18541 tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg ggtagcgtcg
    18601 gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac gattaccgat gcgttttttg
    18661 aatctttctc tggcctgacc accacggggg ccactacgct ggtggggctg gattcgctcc
    18721 ctcacgccat cctcttttat cgccagatgc tgcaatggtt tggcgggatg gggatcatcg
    18781 tgttggcggt tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag
    18841 aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa
    18901 ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg tggtttgctg
    18961 gaatggatgc ctttgatgcc atcggccata gctttgcgac tatcgctatt ggcggcttct
    19021 cgacacatga tgccagtatc ggttatttcg acagcccgac tattaacact atcattgcta
    19081 tcttcctgct gatctctggc tgtaactacg gtctgcactt ttcactgtta agtgggcgta
    19141 gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt tatcggcgta cagttttcac
    19201 tggtggttat ttgtacactc gtactgtggt ttcataatgt ctacagttcg gcgctgatga
    19261 caattaacca ggcgtttttc caggtggtgt cgatggcgac aaccgccggg tttacaactg
    19321 acagcattgc ccgctggccg ctctttttgc cggtactgct tttatgttca gcttttatcg
    19381 gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg ttgctgttta
    19441 agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa cgccgtctat agcattaagc
    19501 tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt ttggggattt ttctccgcct
    19561 atgcattggt gtttattgtc agtatgctgg cgattatcgc cacgggcgtg gatgactttt
    19621 ctgcctttgc ctcggttgtt gcaacattga ataacctggg gccagggctt ggcgtggttg
    19681 ctgataactt taccagtatg aacccggtgg ctaaatggat cctgattgcc aacatgctgt
    19741 ttggtcgtct cgaggtcttt acattgctgg tgctctttac cccgactttc tggcgtgaat
    19801 gatggagtaa tacgtgaaaa cattaattct tttctcaaca agggacggac aaacgcgcga
    19861 gattgcctcc tacctggctt cggaactgaa agaactgggg atccaggcgg atgtcgccaa
    19921 tgtgcaccgc attgaagaac cacaatggga aaactatgac cgtgtggtta ttggtgcttc
    19981 tattcgctat ggtcactacc actctgcgtt ccaggaattt gtcaaaaagc atgcgacgcg
    20041 actgaattcg atgccgagcg ccttttattc cgtaaatctg gtggcgcgta aaccggagaa
    20101 gcgtactcca cagaccaaca gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc
    20161 cgatcgctgt gccgtcattg ccggggcgct gcgttaccca cgttatcgct ggtacgaccg
    20221 ttttatgatc aagctgatta tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt
    20281 tgtctatacc gattgggagc aggtggcgaa tttcgcccga gaaatcgccc atttaaccga
    20341 caaaccgacg ctgaaataag cataaagaat aaaaaatgcg cggtcagaaa attattttaa
    20401 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    20461 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaaagcaaaa
    20521 aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac cccgcgccgc tgagaaaagc
    20581 gaagcggcac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
    20641 attcttgacg tcgcaagacg aaaaatgaat accaagtctc aggagtgaac acgtaattca
    20701 ttacgaagtt taattctt
//
LOCUS       DAJOTB010000082        20606 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJOTB010000082 DAJOTB010000000
VERSION     DAJOTB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.082"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            299..688
                     /locus_tag="OHN28_002925"
     CDS             299..688
                     /locus_tag="OHN28_002925"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2298291.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            685..1032
                     /gene="tnpB"
                     /locus_tag="OHN28_002926"
     CDS             685..1032
                     /gene="tnpB"
                     /locus_tag="OHN28_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2298292.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1082..2356
                     /locus_tag="OHN28_002927"
                     /pseudo
     CDS             1082..2356
                     /locus_tag="OHN28_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2396..2662
                     /locus_tag="OHN28_002928"
                     /pseudo
     CDS             2396..2662
                     /locus_tag="OHN28_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(2649..3100)
                     /gene="def"
                     /locus_tag="OHN28_002929"
                     /pseudo
     CDS             complement(2649..3100)
                     /gene="def"
                     /locus_tag="OHN28_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            complement(3209..7342)
                     /gene="hbp"
                     /locus_tag="OHN28_002930"
     CDS             complement(3209..7342)
                     /gene="hbp"
                     /locus_tag="OHN28_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-binding protease autotransporter Hbp"
                     /protein_id="HCQ2298293.1"
                     /translation="MNRIYSLRYSAVARGFIAVSEFARKCVHKSVRRLCFPVLLLIPV
                     LFSAGSLAGTVNNELGYQLFRDFAENKGMFRPGATNIAIYNKQGEFVGTLDKAAMPDF
                     SAVDSEIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVDRNNAPSLDFHAPRLD
                     KLVTEVAPTAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTKMGGAYSWLT
                     GGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEGGALEWSFNSSTGAGA
                     LTQGTTTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSN
                     GSTWTGAGIVVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVL
                     NQQADNKGQVQAFSSVNIASGRPTVVLTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQ
                     LKAADYGAVLANNVDKRATITLDYALRADKVALNGWSESGKGTAGNLYKYNNPYTNTT
                     DYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNTAGYLFHGQLKGNLNV
                     DNRLPEGVTSALVMDGAADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHS
                     VLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTIQADNSSVTLGDSRVFI
                     DKNDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVN
                     ISSDSAVLGNSTLTSTALNLNKGANALASQSFVSDGPVNISDATLSLNSRPDEVSHTL
                     LPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQML
                     YSLFNGYRNIWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTT
                     LTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSNKDTLDIPLVS
                     APEATADNLFRASTRVVGFSDVTPILSVRKEDGKKEWVLDGYQVARNDGQGKAAATFM
                     HISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADR
                     KHELGSMDLFTGVMATYTDTDASADLYSGKTKSWGGGFYASGLFRSGAYFDVIAKYIH
                     NENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNW
                     NDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKD
                     AAGEHQINGRKDSRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF"
     gene            7513..7703
                     /locus_tag="OHN28_002931"
                     /pseudo
     CDS             7513..7703
                     /locus_tag="OHN28_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(7671..7886)
                     /locus_tag="OHN28_002932"
                     /pseudo
     CDS             complement(7671..7886)
                     /locus_tag="OHN28_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7982..8239
                     /locus_tag="OHN28_002933"
                     /pseudo
     CDS             7982..8239
                     /locus_tag="OHN28_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8307..9458
                     /locus_tag="OHN28_002934"
     CDS             8307..9458
                     /locus_tag="OHN28_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HCQ2298294.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELVRHLEFTVSAGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            9580..9945
                     /locus_tag="OHN28_002935"
     CDS             9580..9945
                     /locus_tag="OHN28_002935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298295.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVTPPV"
     gene            9945..10207
                     /locus_tag="OHN28_002936"
                     /pseudo
     CDS             9945..10207
                     /locus_tag="OHN28_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706936.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            10412..11422
                     /locus_tag="OHN28_002937"
     CDS             10412..11422
                     /locus_tag="OHN28_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCQ2298296.1"
                     /translation="MFQLGIDVSKKTLDLCLLREGVKGSVKTRKLQNDINAVNAVIAW
                     LSKQHCKPEDVHIIMEATGVYHESLAYGLHKAGGRISLANPHRAREFARGMGMLTKND
                     RVDAYMLACYGALKAPEPWLPPPEEVRHLSALLRRRDALVADSTREKNRLEKYLATDT
                     PSVIAESVEAMIGQLNAEIKRLDRLIRSHLNQHPELKKDFDLLTSIKSVGYQLGLNML
                     VILRSHDFATAEQAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCGLR
                     FNPLMKAMYERLCLRGKGKMCAIGALMRKLVHWCYGVLKTQKPFDVGYLSSYARSACK
                     AA"
     gene            12000..12590
                     /locus_tag="OHN28_002938"
     CDS             12000..12590
                     /locus_tag="OHN28_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2298297.1"
                     /translation="MSRIFAYCRVSTLEQTTENQRREIESAGFNVRPQRIIEEQISGS
                     VAASERPGFNRLLDRLESGDVLIVTKLDRLGRNAMDIRKTVEQLTESGIRVHCLALGG
                     VDLTSSAGKMTMQVISAVAEFERDLLLERTYSGIARARDAGKRFGRPPLLNEEQKQKV
                     LERIKSGASISAIAREFRTTRQTILRVRAKQQTVTV"
     gene            12637..12795
                     /locus_tag="OHN28_002939"
                     /pseudo
     CDS             12637..12795
                     /locus_tag="OHN28_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12945..13943
                     /locus_tag="OHN28_002940"
     CDS             12945..13943
                     /locus_tag="OHN28_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2298298.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGVCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            13943..14980
                     /locus_tag="OHN28_002941"
     CDS             13943..14980
                     /locus_tag="OHN28_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2298299.1"
                     /translation="MSVGAIETSRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGSGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTTASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            14980..15741
                     /locus_tag="OHN28_002942"
     CDS             14980..15741
                     /locus_tag="OHN28_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298300.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVACVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            15798..16985
                     /locus_tag="OHN28_002943"
     CDS             15798..16985
                     /locus_tag="OHN28_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298301.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(17063..17320)
                     /locus_tag="OHN28_002944"
     CDS             complement(17063..17320)
                     /locus_tag="OHN28_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCQ2298302.1"
                     /translation="MEKKTISDYTEAEFLEFVRKICRAEGETEADDDKLVDEFRRLAE
                     HPDGSDLIYYPRDDREDSPEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(17321..17554)
                     /locus_tag="OHN28_002945"
     CDS             complement(17321..17554)
                     /locus_tag="OHN28_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCQ2298303.1"
                     /translation="MEVGKDPELLKQFKNQNKVLVTKGKSSFVPESERVGERERFELH
                     HIKRVTDGGAVYDIDNLRVVTPKHHIEIHRGNK"
     gene            17650..17921
                     /locus_tag="OHN28_002946"
                     /pseudo
     CDS             17650..17921
                     /locus_tag="OHN28_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18082..18261)
                     /locus_tag="OHN28_002947"
                     /pseudo
     CDS             complement(18082..18261)
                     /locus_tag="OHN28_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(18307..18474)
                     /locus_tag="OHN28_002948"
                     /pseudo
     CDS             complement(18307..18474)
                     /locus_tag="OHN28_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            18471..19301
                     /locus_tag="OHN28_002949"
                     /pseudo
     CDS             18471..19301
                     /locus_tag="OHN28_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(19345..19993)
                     /locus_tag="OHN28_002950"
                     /pseudo
     CDS             complement(19345..19993)
                     /locus_tag="OHN28_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            20050..20481
                     /locus_tag="OHN28_002951"
                     /pseudo
     CDS             20050..20481
                     /locus_tag="OHN28_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
BASE COUNT         4924 a         5469 c         5308 g         4905 t
ORIGIN      
        1 agcatccagt ctgttgcccg acatggcgaa caataacgcg gtgcatgtta acgaaaaaat
       61 cataccgaat gtggccgccg gtctgataaa agttaaaccc caggcagagc gtttcaccgg
      121 agaaggcgct attaagttcg tcgacgcatc tcaggaaaaa tatgatgtaa taataacctg
      181 tacaggctat gaaatgcctg attactcgtt cattcgtaag cgtacagcct gaaccgtctg
      241 gtcagaatct gacgaattag acaaagtggt gtccaccaaa taagtagtgg gaaccaaagt
      301 gtcagatatg cagaaaaatg tgactcccgg caggcgaaaa ggctgcccta attatcctcc
      361 cgaatttaaa cagcagctcg ttgctgcctc ctgtgaaccc gggatatcca tctcaaaact
      421 tgctcttgaa aatggcatta acgccaatct gttgttcaaa tggcgacaac aatggcgcga
      481 gggaaagctg ctattacctt cttcagagag cccccagcta cttcctgtga ctctcgatgc
      541 agctgccgaa cagccagaat cgcttgcaga ggacccggaa accctcagta tcagctgtga
      601 ggtaacgttc cggcacggga cgctccgctt caatggcaat gtcagcgaaa agctcctgac
      661 tctgctgata caggaactga agcgatgatc ccgttacctt ccgggaccaa aatttggctg
      721 gttgccggta tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa agtacagacg
      781 gcgctgaaag acgatcccat gtccggccat gttttcattt tccggggccg cagcggcagt
      841 caggttaaac tgctgtggtc caccggtgac gggctgtgcc tcctgaccaa acggctggag
      901 cgtgggcgct tcgcctggcc gtcagcccgt gatggcaaag tgttccttac gcaggcgcag
      961 ctggcgatgc tgctggaagg tatcgactgg cgacagccta agcggctgct gacctccctg
     1021 accatgctgt aaatctcttt atcctggttg tcacagaata agcccggtaa aatacgggct
     1081 tatgaacgac atctcttctg acgacatctt cctgctgaaa cagcgcctgg ccgaacagga
     1141 agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa tagaccatct
     1201 gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg aaaaagtctc
     1261 ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga gcgatacgct
     1321 gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc gcacccgtaa
     1381 gccgttccct gaatcactac cccgtgacga aaagcgactg ttgcctgcgg cgccgtgctg
     1441 cccgaactgc ggcggttcac tgagctatct gggcgaggat accgccgaac agctggagtt
     1501 gatgcgtagc gccttccggg ttatccggac ggtacgggaa aaacatgctc catgccgatg
     1561 ataccccggt ccaggtactg ctgccgggta ataagaagac gaagaccggg cggttgtggg
     1621 cgtatgttcg tgatgaccgc aatgccgggt cagcgttggc acctgcagtg tggttcgctt
     1681 acagcccgga caggaaaggc atccatccgc agactcatct tgcttgcttc agcggtgtgc
     1741 tgcaagcgga tgcgtacgcc gggttcaacg agctgtatcg caatggtcgg ataacggaag
     1801 ctgcctgctg ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc atcccgtcag
     1861 cactgacgga agaagccctg gagcagatcg gtcagttgta cgccatagag gcggatataa
     1921 ggggaatgcc ggcagagcag cggcttgctg aacgtcagcg aaaaacgaaa ccgctgttga
     1981 aatccctgga aagctggttg cgtgaaaaga tgaagaccct gtcgcgacac tcagagttgg
     2041 cgaaggcgtt cgcgtacgca cttaaccagt ggccggcact gacgtactat gcgaacgatg
     2101 gctgggtgga aatcgataac aacatcgctg aaaatgccct gcgggcggtc agtctgggtc
     2161 gtaaaaactt cctgttcttc ggctctgacc atggtggtgg cggggagcgc tactgtacag
     2221 cctgatcggg acgtgcaaac tgaatgacgt ggatccagaa agctaccttc gccatgtgct
     2281 tggcgtcata gcagactggc cggtcaaccg ggtcagcgaa ctgcttccgt ggcgcatagc
     2341 actgccagct gaataacaca tccccgtcaa tacggccctc gctgtacgct taccaataaa
     2401 taatatgctt atcgacctga tggctgcaat gtcgcataaa gactggctga gtcgtcgtca
     2461 acgacaaaaa cagggaattg aacgtgccca tactttaggt aagtatcggg gcaaacaggc
     2521 agatcgggaa cgacatcaga aagtaatgta ctaccggcag atcaagaaac tgagcatccg
     2581 tgaaacggca gaggtgacag gctatagtac ttcccaagtt tgtcgcattc aggcgctcta
     2641 taaagaggtt aaacctgatt aaaaaatagc cttaacgtac aatattttcc gtttcccaaa
     2701 cggagcccaa tacagatggc taacagaccg tctgcttcca gttcaaatgg tttaccgtcg
     2761 cggtccaggg cgcgaatttt aactttctct gcgcgtggca ctaaagctcg ttgttcaggg
     2821 attgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     2881 attaacacca gccgttcgtc acggttttcc gaaacatcaa ttacaatgat acgttgatgg
     2941 atatcaacct gggttgccgc caggccaata ctttttctgc gtacatcgtc tcgaacatat
     3001 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
     3061 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
     3121 aggagttaga aagattattt cttctactgt accgtaatca gataatcgca gcaaagggac
     3181 aaagcaatag tccctttgct gcacagcatc agaatgaata acgaatatta gcgtttatcg
     3241 catcatctgt gttgtattta ccaaatgcag agcgttcaac ttccagcccc agacgcgtat
     3301 tgtcgccaaa ccgggcattt aaccccacac cgtaaagcat acgactgtct tttctgccat
     3361 taatctgatg ttctcccgct gcatccttca gatgaacgtc agcactgtcc gtcagatcga
     3421 actcataatg caggccggca cgggctgtca gactccagtc cttaccactg aaggttttac
     3481 cggaaacaac gccggttctg cctaccagag gattaacgct gttacgacgc attgagacat
     3541 ccattccact gtcgttccag ttaaatgttt ggccctgcag tcttccccag accagttccg
     3601 cctgaggttc aacaaacgtc gtatctgtca gatgataacg gtatccgact tctgcacctg
     3661 catacagtga atggctgcgg aagttctgtt taccagctcc ggcaaagttc aggtcatatt
     3721 tgttttcatt gtgaatatat ttggcaatca catcaaagta agcgccggac cggaacagac
     3781 cactggcata gaaaccacca ccccatgatt ttgttttacc gctgtacagg tctgctgacg
     3841 catctgtgtc agtgtaggtg gccatcacgc cggtaaacag gtccatactt cccagttcgt
     3901 gcttacggtc agcccccatc tgcagcaggg tatagtggtc agtgaaaccg ccatcagcag
     3961 agccggaacc gttcagcaga cgcacccacg taccggcttc gccattaata tccctcaaat
     4021 cgcccatgcg tttgttcagg ttgttaactt cagtgatgaa gttgttatag ctgatgtgca
     4081 tgaatgtggc ggcagcctta ccctggccgt cgttacgtgc aacctggtaa ccatcgagga
     4141 cccactcttt tttcccgtcc tcttttctga cactaaggat gggggtgaca tcactgaatc
     4201 ccacaacccg tgttgatgcc ctgaacagat tatcagctgt cgcttcaggt gcgctgacca
     4261 gtggaatatc aagcgtgtcc ttgttagaag gttttttcag gaagttaacc cagatgctgt
     4321 tgtcatgacc tgttgccgac ttgtttatca ccagtttgtc tgccttgtta aggtctgtac
     4381 gcatgacaaa tgctgactga accgcgtcca gattatctgt tgtcagtgtc gtgaacggtg
     4441 atgttccccc gttaaaaccg actattgtcc ggttaagttt catatttcct gccgtggagt
     4501 ttccgttcat cgaccactgg gtgtctgtca tgctgacggt ggcatccggt gcattcaggc
     4561 tcccgctcca gatattgcgg tacccgttaa acaggctgta caacatctga ttctgaagag
     4621 tcaggtcagg actcagttcc ccttcccctc cgagggtgac agtcccttta tcctgaacat
     4681 tgatattacc tgacaacata ctgtacggcc ccacgttcag gcgggcatcg tctcccttca
     4741 ggttccatga accggcataa tcgtatacag gtaaaagtgt gtgagatacc tcatcaggac
     4801 ggctgttcag actcagggtg gcatcagaaa tattcactgg accgtcagaa acaaaactct
     4861 gactggccag agcatttgct cccttgttca gattcagggc ggtactggtc agtgttgagt
     4921 tccccagaac ggcactgtct gaggagatat tcacggtact gttgttcgcc tgtattccgc
     4981 cattgaatat atcattgata ttcagcactg actgattatc caggttgacg gtgccgttga
     5041 agacactttt atctgcatct ttagttgcaa cagatgtgcc ttcttcaagg gtaaaggctg
     5101 ttccctggcc atcgtttttg tcgataaata cccggctgtc gcccagcgtg acgctggagt
     5161 tatctgcctg gatggttgtg ttcagtgtgg cattgcgacc aagaccaaaa tcagtgttct
     5221 tcagtgacag cctgtcaaag gtaaaactgc ggttctccca gtcctcctga ctgaatgacg
     5281 taggctgagt cagaaccgaa tggtctccac tggcagccag tttgtcagcc acagactgag
     5341 tattgtatgc atggataacc ggatgcccct gcagcgtcag acgcccgttt tcctgggtga
     5401 atgtaccgga gatatccgca gctccgtcca tcaccagagc actggtaacg ccttcaggca
     5461 ggcgattgtc cacattcaga ttgcctttca gttgtccgtg aaacagatac cctgcagtat
     5521 tgaaacggtc agctaccagt ttctgtgcat ccccctgact gtgccccaca aactcccagg
     5581 tggcgttgct gctctggtcc gtggggaaat aaccataggt gctctgcttc aggatgaagt
     5641 aatccgtcgt atttgtgtac gggttattgt atttatataa atttccggca gttcctttac
     5701 ctgattccga ccagccattc agtgctactt tgtcagcccg cagggcatag tccagcgtga
     5761 tagtggcccg tttatcaacg ttattcgcca gcacggcacc ataatctgcc gccttcaact
     5821 gatgaaacgt cagactgtta ccattaacat ccagtgtgcc cccacgatat ccccatgaga
     5881 cggtatccgg atttacctgc cgctcatcag tcagtaccac ggtcggccgg ccactggcaa
     5941 tattaacact gctgaacgcc tgcacctgtc ctttattgtc cgcctgctgg ttcagtacaa
     6001 cctttccgtc cccgaccttc aggccacctt cattaatacc tgtaccctgt accgtcagcg
     6061 taccttcacc aattttatgc aggttatcgc ccttaacacc attaacctgc cagtttacgg
     6121 acaccccgtt gtccacaaca ataccggcac cggtccaggt acttccgtta gaggttgtta
     6181 ctgtgtaatt atcacggaac gtcagggaac ccgccccctg agaaaccgaa tccttaaggt
     6241 taatctgacc attctgcccc tgaaatatca ggttcttacc agcattcagg tcatttccct
     6301 gctgcccgtg catggcatat gtggtggttc cctgtgtcag cgcaccagct ccggtactgc
     6361 tgttaaagct ccactccagt gcaccacctt ccgatgttct gaacgtgacc ggggcatcat
     6421 tgtcttcatt aaatttctgc ccgataaaat ccagtggaat aacagcccag ttatttcccc
     6481 tgccccccgc gccattcccc gcagtaagaa caccgaccag cacccattta ttctgaacag
     6541 tatcaaaagc aaataaaggc gaaccgctgt caccggcctc gccataaatg ggcattgcac
     6601 cattaagttt gtaatcaaaa accagacctg aactggtgct aatcatttct ccattctgat
     6661 aggatgacag gctaccgaca gtcccgccgg tcagccagga atatgcacct cccatttttg
     6721 tcagctgtcc gttactgtcc ttaatatact gagtaccaga ccccagacga taaaaaacag
     6781 gatagcgctc cttatccaga tatgcgccag ccactgcccc ctgcgccgtc accgcagtag
     6841 gggcaacctc tgtcaccagt ttatccagcc ggggggcatg aaaatccagt gacggcgcat
     6901 tattccggtc cacgatattg taacggtttt caccatcacc aaagctaacg tttgtatatc
     6961 ccccgttatg tttcacgctg gcgatatact gcgggtttat cagtgtcgcc acaccgattt
     7021 ccgaatccac agcactgaaa tcaggcatag ctgccttatc cagcgtaccg acaaattctc
     7081 cctgcttatt ataaatagcg atattcgttg cccccgggcg gaacatcccc ttattttcag
     7141 caaaatcacg aaataactga tacccgagtt cattattgac cgttcccgca agacttcctg
     7201 cagagaatag taccgggatc agtaataaaa ccgggaaaca cagacgtctg acagacttat
     7261 gaacacattt cctagcaaac tcagatacgg caataaagcc ccgggccaca gcgctgtagc
     7321 gaagagaata aattctgttc atttttaatt actcctgaga aaaataaaat cagtactact
     7381 caggcataac ggataatcac catcatcctg aggaatcccc agaaaaagga gacaggcata
     7441 gcgtgatgtg aactactgat tctttcacaa attcagggag agcaccgtta tgcctgcaca
     7501 taaagtatgc cagaaccggt ggcgaatgtg tgctatgcac aaatggtgaa acagttcctg
     7561 agtcgtgacc cgctcgaatg cgtgctgtgt ggcggccgga tggtataccg gcgtgccata
     7621 gcggggctga atgtggacgg gctgaagaaa acgcgcggga tatcagtctg ataaggatta
     7681 ctttccagcc ggctgatggc tgacgggcgt aatcccattc gttcggccag cgcagacttt
     7741 gttaaccccg ctttttctct catttcgtat aacatttcta ccatctccag ttctttgtca
     7801 gcatcttcat accctttaac agcttctgga gtattaagca gtttttgctt tacctggctg
     7861 aacggaacac tttttacttt cgtcatttca tctccttcag gcgcatcctt gaagtggttt
     7921 actgaatttg gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc
     7981 aatgaaaaaa agaaatttca gcgcagagtt taaacgcgaa tccgctcaac tggttgttga
     8041 ccagaactac acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac
     8101 aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat
     8161 aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca ttgaaatgga
     8221 gaatgaaata ttaaaaaagg ctactgtaga ttcaattggt caacgcaaca gttatgtgaa
     8281 aacatggggt tgcggaggtt ttttgaatga gacgaacatt tacagcagag gaaaaagcct
     8341 ctgtttttga actatggaag aacggaacag gcttcagtga aatagcgaat atcctgggtt
     8401 caaaacccgg aacgatcttc actatgttaa gggatactgg cggcataaaa ccccatgagc
     8461 gtaagcgggc tgtagctcac ctgacactgt ctgagcgcga ggagatacga gctggtttgt
     8521 cagccaaaat gagcattcgt gcgatagcta ctgcgctgaa tcgcagtcct tcgacgatct
     8581 cacgtgaagt tcagcgtaat cggggcagac gctattacaa agctgttgat gctaataacc
     8641 gagccaacag aatggcgaaa aggccaaaac cgtgcttact ggatcaaaat ttaccattgc
     8701 gaaagcttgt tctggaaaag ctggagatga aatggtctcc agagcaaata tcaggatggt
     8761 taaggcgaac aaaaccacgt caaaaaacgc tgcgaatatc acctgagaca atttataaaa
     8821 cgctgtactt tcgtagccgt gaagcgctac accacctgaa tatacagcat ctgcgacggt
     8881 cgcatagcct tcgccatggc aggcgtcata cccgcaaagg cgaaagaggt acgattaaca
     8941 tagtgaacgg aacaccaatt cacgaacgtt cccgaaatat cgataacaga cgctctctgg
     9001 gacattggga gggcgattta gtctcaggta caaaaaactc tcatatagcc acacttgtag
     9061 accgaaaatc acgttatacg atcatcctta gactcagggg caaagattct gtctcagtaa
     9121 atcaggctct taccgacaaa ttcctgagtt taccgtcaga actcagaaaa tcactgacat
     9181 gggacagagg aatggaactg gtcagacatc tagaatttac tgtcagcgcc ggcgttaaag
     9241 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaac acaaatgggc
     9301 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catgaactag
     9361 atctggttgc tgctcagcta aacaacagac cgagaaagac actgaagttc aaaacaccga
     9421 aagagataat tgaaaggggt gttgcattga cagattgaat ctacaaccgc gctcttgatg
     9481 tcagactccc tcgagtaggc agcctggcgg ctgcggcttg tcatggcctg gaattaccgt
     9541 tataaaaaaa gataatgtca ttgtctttca ggtagttata tggcccgttc agctaaaccc
     9601 cgtaaacgca aacccgcacc acaaagaagc aaacttcccc gctatgttgt gaaacttcat
     9661 ccggatgatt tttttgacga agaagacgct gaagttctgc gctttgataa ttttgacgat
     9721 gccgttgagt gctgcgctga cctgggtatt ccgttctttc tggatgcagg aaacaaaaag
     9781 ctggtcttct ggtttgttcg tgtcgatgac gaagggtatc cggaaatagc ccgctgtacg
     9841 gagcgggagt ttgcaaccat tcttgccggt atcagtgccg gtggtatgta ctgcccggaa
     9901 tgcggcacag ttcactggcc ggatggcgtt accccacccg tctgatgctt ccccgttttg
     9961 ccgatatttt tcagcagggt aaccgctggc ttaactggct ggagaaacag ccggaaggtt
    10021 cagtgcgtcc ggtggtgact gagtcagtga caaaaatcat ggcatgcggg accacgctga
    10081 tgggctacac gcaatggtgc tgttcgtcac cggactgttg ccacaccaaa aagatctgct
    10141 tccggtgtaa agccgctcct gtccgaactg cggggtgaag gctggcgcac agtggataca
    10201 gtatcttgta tgtaatagga cttgatggct agctaccatc atatgaagca gagtgagctt
    10261 gtcccagccc tgaacagtca gactttgatt agcagcaaaa gccctctgct tcacctttca
    10321 cgccagcatt aacattgaac ggttgccaag agcgatgact ctgcatgtac aaggggaata
    10381 ggcgtgatgg tttggtgaaa ggagaatatt catgtttcag ttaggtattg atgtcagtaa
    10441 gaaaacgctg gatttgtgcc tgctgcgcga aggcgtaaaa ggcagcgtta aaacccgaaa
    10501 gcttcagaat gatattaatg ctgtcaacgc ggtaatagcc tggctcagta agcaacattg
    10561 taaacctgaa gatgtccata tcatcatgga agccactggt gtttatcatg aatcactggc
    10621 ttacgggctt cacaaagccg ggggccggat atcacttgcc aacccacaca gggcaagaga
    10681 gtttgcccgg ggtatgggaa tgctgaccaa aaatgaccgg gttgatgctt atatgctggc
    10741 ttgctatggc gcgcttaaag caccggagcc atggcttcct ccaccggaag aagtccgcca
    10801 tctcagtgca ctgctccgca gacgtgatgc tcttgtggct gacagcactc gcgaaaaaaa
    10861 caggctggaa aaatacctgg caacagacac accttctgtt atagctgaat ccgtagaggc
    10921 aatgattggt cagttgaacg cagaaattaa acgtctggac agactgatcc gttcacacct
    10981 taaccagcat cctgaattaa aaaaggattt tgacctgctg acgtcaataa aatccgtagg
    11041 ttatcagtta ggtctgaata tgcttgtcat cctgcggagc catgatttcg ccacagcaga
    11101 acaggcggct gcatttttag gtgtcgttcc cgttgaaaaa tattccggca cctcggtcag
    11161 agggtgtccg aaactgtcaa aaattggtcc gccggaaatc cgggcgaaac tgtatctggc
    11221 ttcgttgtgt ggtctgagat ttaacccatt gatgaaagcg atgtatgagc gattatgcct
    11281 gagaggtaag ggcaaaatgt gtgccattgg tgcgctgatg agaaaactcg tgcactggtg
    11341 ctatggcgta ctgaagaccc aaaaaccgtt tgatgtgggc tatttgtctt catatgcgcg
    11401 tagcgcatgt aaggcggcat gatgtagtta catatggtac aggagtaaca ccgccgaagc
    11461 ccggcgtaag ccggtacgta ttgataggtt tcacctatcc catccctgcc ctgtgaggcc
    11521 atgcgggctt tcagcgatga tgaagtacag gtaagccctt ttaaccccgt tagcgcggct
    11581 acgccgcgct gtatcgggga cttagtgctg acagcttgct ggcagtactt agggggctgt
    11641 acgtcggagt tcaagcgatt tttcttgaca gaacacggta gctgctaagc catacccgct
    11701 ttcagccatg agagagcttc tgtgcgcggt cggagtggtc ccgacgaggg tttacccgaa
    11761 gtcgggacgt atctccgcgt tagcggggtc agtttggaat tcggaaaata aaccatgtta
    11821 agactatttt ttagccaaag ggctttcagg aatcgccaga atggcgggac aacagtggtt
    11881 ttggtgtccg aaatcgaccg gtttcggaca ttgttaaaca gatatatccg aaagtcagcg
    11941 aaattacttt cggacatgca ttataatatg tacacccatt tcgtacagaa gatctcacta
    12001 tgtctcgaat ttttgcctat tgtcgggttt ccactctgga acagacgacc gaaaatcaac
    12061 gtcgggaaat cgaaagcgcg ggttttaacg tcagacccca acgaataatc gaagaacaaa
    12121 ttagtggatc agtagcagcc agtgaacgtc caggttttaa ccggttgctt gatcgtctgg
    12181 aaagtggcga tgtattgata gtcacaaaac tggatcgcct cggtcgtaat gcaatggata
    12241 tcaggaaaac agtggagcaa ctgaccgaat ctggcatcag ggtgcattgc ctggcattag
    12301 gtggtgttga tctgaccagt tcggcaggaa aaatgacaat gcaagtgatt tcagcggtcg
    12361 ctgaatttga acgagatctt ttacttgaac gaacttattc agggatagca agagcacggg
    12421 atgcaggaaa acgttttggt cgcccaccgc tattaaatga agaacaaaaa cagaaggtac
    12481 tcgaacgcat taagtctggg gcaagtataa gtgctattgc cagggaattc cgaaccacac
    12541 ggcaaacaat tctgagagtt agagcgaaac agcaaactgt tactgtatag aaaatagtct
    12601 tagcgtggga tattttccgt tttccaagcg gaccccaagg aagactacat cgcgttcatg
    12661 ccgaaaccga atgtaagaac ggcattgcat aatcttgcag tggcgatcga acattacaat
    12721 gaaaaccatc cgcacagtgc gttgggttat cgctctccgc gagaatatcg acgtcagcgg
    12781 gtaacattaa cttaagatac aacccctgtc tggaaataag ggggcaagaa cacattgaca
    12841 ctataaatat ggcattgtat aaaaccccgc gataataatg agtttcattt gcattactcc
    12901 catttgctgg tcatccactc gcctgtttac aaggatagcg tttaatgaag aaggtccttt
    12961 gcgcgttagg cttgatgttc accgccgtca gttccgcgct ggcaacaacc taccccctga
    13021 cgattgaaaa ctgcggctat caggagacct tcacccggcc gccggagcgc gtggtcgcgc
    13081 tggggcaaaa tacggtcgaa attctgctgc tgctcgggct gcaaaagcag gtggtcgcca
    13141 gcgccttctg gccgaccagc gttttaccgc agctggctga gcaaaacgcg aagattaaaa
    13201 ccttgacggt ggaaattccg agcctggaat cggtgctggc gcaaaatcct gatttcgttc
    13261 ccgcccagtt gccgctgctg ctggggccgg aaagcaaagt cgctaggcgt gaagatctcg
    13321 ccaccgtcgg cgtgaacagc tacgtctcac cgggggtgtg cgccaccaaa aaggccaccg
    13381 gcgatatgta cggcagccgg caaaagctgt gggatatgac ctggctgtat caggaaatta
    13441 gcgatttttc ccgcattttc aacgtcgaag atcgtggtca ggcgctgatt gccgatttca
    13501 aaaaacgtga agccgacctg cgccaggagt ttggtaaaag caaaaaggat ctctccttcg
    13561 tcttctggtt ctccagcgcc tcaccttcgg ccgatgccta tgtcggcggc aaaaacagcg
    13621 cctccggatt tatcgccagc gtgctgggcg gacataatgc gattacctcc gaaaccgagt
    13681 ggcccacggt gagctgggag agcattattg ccgccaaccc ggatgtgatc gtggtcgcca
    13741 gcctagatcg taaccgctgg gcgctggata aagccgagga gaagatcaaa ttcctcaaaa
    13801 gcgatcccgc cgtcagccag ctggaggcgg tgaaaaaagg ccatatcgtg gtcatggacg
    13861 gtcaggcgat gaatccgacc attcgcacgc tttacggcgc ggagcaggtg ggcgagcagc
    13921 tgagaaaaat ggggctgaac tgatgagcgt cggcgcgatt gagacgagcc gcagcatact
    13981 gctcaccggc tggtgtgtac tggcagcgat cgtgctggcg ctggtgatcg ctgtgggcgt
    14041 cagcgttggc gagctggcca tcccgctgca gaatgtcttt tatgctatca gcaacagaac
    14101 cggcttaacc gctgagccgc tcaaccgtat ctatgagagc gttatctggg atttccgcct
    14161 cagccgcgcg ctggtcgccg cctgctgcgg cgccggcctc gctatctgcg gtgtcgtgct
    14221 gcaaagcttg ctgaagaatg ccctcgccga accctacgtc ctcggcgtct ccgccggggc
    14281 ctccaccggc gcggtgtcga tcgtggtgct gggccttggc tctggcgcaa tttccctctc
    14341 ggcaggcgcc tttgccgggg ccttcgccgc cttcgccttt gtcgccttgc tgactaacgg
    14401 cgcgcgcggc ggcaatgagc gtacgattct tgcgggcgtc gccgcctcgc agttatttaa
    14461 cgccatcacg gcctatacca tcagcaccac cgccagcgcg cagcaggcgc gcgatgtgat
    14521 gttctggctg ctgggcagct tcagcggcgt ccgctggccc gaattccagt tggtcatcgt
    14581 ggtggtgctc gcgggtctgg cggtctgcct gtggtatgcc cgggcgctgg acgcgttcac
    14641 cttcggcgac gatgccgccg cctcgctggg gattgctgtg ccgcgcgtgc gcctgatcct
    14701 cttcaccact gcggcgctga tcaccgccac catcgtcagc atggccggct caatcggctt
    14761 tgttggcctg gtggtgccgc acgtgatgcg tttcttcttt ggcccgctgc accgaacgtt
    14821 gctgatcgcc agcgcgctgg caggggcgat attgatggta ctggcggaca ttgcgtcgcg
    14881 cctgctgatt gccccgcaaa gtctgcccgt tggggtagta accgccctgg ttggggtgcc
    14941 tttctttgcc gtgattatct accgctcaag gaataagtga tgagtatttg cgctgaaaat
    15001 atcacctgga aggcaggcaa aaaggtcatc gtcaataatg tctcgctgcg ggtgccgcgc
    15061 ggagaaacgg tcggactgct ggggcccaac ggctgcggca aatcctcgct gctgcgcgtt
    15121 ctggcgggcc tgcgccgccc ggatgcaggt cgcgtcaccc tcgacggcca ggatatcgcc
    15181 cggatggcga aaaagcagct cgcccgccgc gtggcttgcg tcgagcaaca cggcatgacc
    15241 gaggccaata tgcgggtgcg cgacgtcgtg cgcctgggac gcattcccca ccactctccg
    15301 ttctcaaact ggagcgctca ggatgacgaa gcgattgccg ccgcgctgca gcgggtagcg
    15361 atgctggaga aaagcgaaca gggatggtta agcctctccg gcggcgagcg gcagcgggtg
    15421 catatcgccc gcgcgctggc gcagagcccg agcgaaatcc tgctggatga gccgaccaac
    15481 catctggata tacaccatca gatgcagtta atgcagttga tcagcgagct gccggtaacc
    15541 agcattgtgg ccattcacga tcttaaccat gccgcgatgt tctgcgattc gctgatcgtg
    15601 atgcagcagg ggcagatcct cgccagcggg acgccggagg agattttgtc cgaagcgctg
    15661 ctgtgggacg ttttccgggt gaaaaccaaa atcgagatct ccccttacca cggcaaaaag
    15721 cacattcatt tcatcgttta ggttgggcgg atatgcctct tttctccctg cgccctgcgg
    15781 caaccctctg gcctccggtg ttactgggaa gccagttcgt ttttaatatt ggcttttacg
    15841 cggtcgtacc tttcctcgcc ctcttcctgc gcgacgacat gctgctctca ggcggactta
    15901 tcggcctgat cctcgggctg cgtaccttct cccaacaggg gatgtttatc ctcggcggga
    15961 cgctggccga ccgctatggc gctaaggcga tcattctcgc cggctgcgtc gttcgcgtcg
    16021 ccggttttct cctgctggcg tgcggggcgt cgctgtggcc cattatcctt ggcgcctgcc
    16081 tgaccggcgt cggcggcgcc ctgttctccc cgtcgataga ggccctgctg gcgcgggccg
    16141 gtacccacag ccaggccaac gggaaacgca gccgcgccga gtggtttgcg ctgtttgcgg
    16201 tatgcggtga actgggagcg gtgatcggcc cggtggccgg aggcgtgctg agcgggatcg
    16261 gctttcgaca tatcgccctc gccggcgcgg gaatatttct tctggcgctg gcggtgctct
    16321 ttttcggtct gcccgccgac gggcacacga caacaacgcg cagacgagtc ccatggtgga
    16381 cgcctttgcg ccagccgcgc tttgtcgcgt ttattctcgc ctacagctca tggctgttga
    16441 gctacaacca actctacctc gctctgccgg tggagatcca acgttccggc ggccgcgagc
    16501 aggatctggc gccgctcttt atgctggcct cgctgttgat tatcaccctc cagctccctc
    16561 tcgcccgctt cgcccggcga atgggcgccg tgcgcatcct gccggtgggc tttctgctgc
    16621 tgtcggcttc ctttgccagc gtggcgttat tcgccgccgc cccacccgcc gaaagttggc
    16681 tgcggttaat gccggcggct ggttttgtca cgctgctcac cctcggccag atgctgttag
    16741 tgcccgcagc caaggatctg atcccgctgt tcgccgagga gtcgacgctg ggcgcccact
    16801 acggagcgct cgccaccgca ggagggtgcg cggtgttagc cgggaattta ctgctcggtc
    16861 atttacttga ccaggcgctc attccgtcgc cgcaggcggt ttatccctgg cttctgctgg
    16921 cgctgtttcc gctgtgcagc gccgtcgccc tgcgggcgat ctgtcgcccg ctggcggcaa
    16981 catgacgcag agcgggtaac agcccgctct ggaagtgaac agctggtatc catattttca
    17041 gctaatcctt taacaaagat cctcagccct gtttaaagcc cggcttgccg tttttggctc
    17101 gccattcctt cacttctttt acgatacctt caggactgtc ctcacggtca tcccttggat
    17161 aataaataag atcagaacca tcagggtgtt cagctaatcg acgaaattca tccactaatt
    17221 tatcatcatc agcctcagtt tcaccttcag cccggcagat ttttctgacg aactccagaa
    17281 actccgcctc ggtatagtca gaaatcgttt tcttttccat ttacttattt cctctgtgaa
    17341 tttcaatatg atgcttgggt gtcactaccc tgagattatc gatatcataa acagcgccac
    17401 catccgtaac gcgcttgata tggtgcagtt cgaaacgttc tcgttcacct acacgctctg
    17461 attcaggaac aaacgaagat ttaccttttg taaccagaac cttattctga tttttgaact
    17521 gcttcagtaa ctccggatct ttccccactt ccatccacaa cgccttacgg aaccacggca
    17581 gcctggaggc tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa
    17641 tgagttccca tgtcacacaa cctagagcac cagaaagtcc acaccagaat ggtgaaagag
    17701 gttctgaaag ccgtcgccag agccaataat cacccttatc agtccgtatt tacagacttt
    17761 atcgccggtc atccctcctg cacggtctgt ttctgggaaa ccttcaacaa aatgtacccg
    17821 gattcaccgt acgaatacgt cacgttctgc catacctgcc gtcgtttgat ttatacgaaa
    17881 cagaagcgga gatgaaggcg gatgacccga agtggtggtg attttcagcc ccgaccgctg
    17941 aaacgcctgt tcacaatgtc gcggtataaa aatcgaccat gcagagagag aaaccaccgg
    18001 agtggtggga aaagcagaaa ggctcacgtg gcataaggcg tggacagcgg tatcgtctgg
    18061 tttttcaatt tcctatacgt gaacacaatc tgcaattcgg tatacagcga acctgccacc
    18121 ataagtgttt ttataccact taactggctc agcaccgggg gcaggcttca ttctgaattc
    18181 atcgcgcaga cgtcctttcg agaaacacct gtcttcggca atctcctgcg cacgtctggt
    18241 gaccagctca tcattattca tggttcttgc atctccgtca gacgttttta tatccggaca
    18301 gcccctctat ggccacttct gtgcagtatg caatacacgc aggatataca cttttcccca
    18361 ctggctgtcg acctcataaa ccagaacaaa atgcgggtga ataaccagtt cccgggtacc
    18421 ttccacacga ccattgcggc ctttatacgg ctgcccggct aggctgctgg ctgaacaccc
    18481 gcaaggttca cactgatgac acaccagtaa aagtactggc accgggcagg aagaaggcga
    18541 aaacaggata tatctggacg tatgtccggg atgaccggaa tgccggttcg ccagaacctc
    18601 cggcggtctg gttcgcctac tcaccggacc atcagggtaa acatccggag caacaccttc
    18661 gtcccttccg gggtatcctg caggcagatg agttcgcagg ttacgatcgg ctgttcagtg
    18721 ccgaacgtga aggcggcgcg ttgacggaag caggatgctg ggctcatgcg cggcgcaaaa
    18781 tccacgatgt atatatcagt accaaaagcg cgacggcgga agaagcactg aaactaatcg
    18841 gcgaactgta cgccattgag cacgaaatac gcgggttgcc ggtgtctgaa cgcctggcgg
    18901 tcaggcaaat gcagagtaaa ccgctactga cttccttgta taagctgatg caggagaaag
    18961 aacacacgtt atcgaaaaaa tgccgtctga gagatgcgtt ccggtatatc aggaagcact
    19021 gggttgcgtt gtgcaacttc tgtgatgacg gtctggcgga ggcggacaat aacacagcgg
    19081 aaagagcgct tcgtgcagtc tgtctcggaa agaaaaatta cgtgttcttc ggtagcgatc
    19141 acggcggcga gcgtggtgca ctgctgtacg ggctgatcgg cacctgccgt ctgaacggta
    19201 tcgatccgga agcgtatctg cgccatattc tgagcgtact gccggaatgg ctttccaacc
    19261 gagttgacga actcctgcca tggaacgtag tactcaccaa tatgaaccgc cccgggtttc
    19321 ctggagagtg ttttatctgt gaactcaggc tgccagatca tcgtttccga tggaagcata
    19381 ataagctttt tctgcttctg ccggaggagt atggcccagc ctttccagca atcgtcgatt
    19441 gttataccag tccacccacg tgagtgtggc cagctccact tctgtccggt ttttccagct
    19501 cttacggtgt attacctccg ctttgtaaag gccattgatg ctctccgcca tcgcgttgtc
    19561 atacgagtca ccagtacttc ctgttgatgc cagtaatccg gcttccttaa gccgctgtgt
    19621 gtaggccagc gatacatact gagaaccttt atcactgtga tggactgtgc cggacggtcg
    19681 acgggcccat aacgcctgct ccagagcatc cagcacgaat gtcgtttcca tagacgatgc
    19741 ggctcagttc acgattttca cgttccagct ctttcagacg ctgacgttca gcggtggtga
    19801 gcccgccatc accgcctccc gtatcccgct catgctggcg tacccagaca cgcagagtct
    19861 tcggtgtaca gccaatcttt ggggcaatgg aacaaattgc cgcccactgt gagtcatatt
    19921 cgccctgact ttccagaacc atacgaatcg ccctctgacg gacttcgggg gaaaaacgag
    19981 tatttttagt cattctgttt acctctttct cagggagttt agtctccagg attcccgggg
    20041 cggttcagta ataccggtga tctcgaggcc cgtttgcaga agaacgggag tgttcgtttc
    20101 cgtgaccgtc ggacgggaga gtttgtctgt acggattatg gtgacagggt tgttttccgt
    20161 aatcaccatg accgcaatgc gctggcagat aaactggatt tgattgcacc tgtattgttt
    20221 gagcgtgatc ccagaatggg ctttgaacca gaaggaaacg acaaacaatt taaccaggtc
    20281 tttgctgaaa tggttgcctg gcataatgtg acaggccgta ccggtcacga agactacagg
    20341 attacacgac cggatgtgga tcatcaccgg gaaggaagtg aacgctacta ccgcgattat
    20401 atcgcggcga acagcaacga tgacgcatca ctgcctccgc cggagcagga taaacgctgg
    20461 gaaccgccat ctcccgtatg acctgtgtcc cttagcgaaa atctcgtagc ttctctctca
    20521 atgagggcgg gtatttgaca tcctccacgc cctaaggacg tgggttcctg cttcaacggg
    20581 cggcatccgt catccgccaa tacatt
//
LOCUS       DAJOTB010000083        20563 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJOTB010000083 DAJOTB010000000
VERSION     DAJOTB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.083"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..282)
                     /locus_tag="OHN28_002952"
     CDS             complement(<1..282)
                     /locus_tag="OHN28_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HCQ2298304.1"
                     /translation="MITHYDIKMETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPEKFR"
     gene            complement(279..3104)
                     /gene="traG"
                     /locus_tag="OHN28_002953"
     CDS             complement(279..3104)
                     /gene="traG"
                     /locus_tag="OHN28_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HCQ2298305.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAEMVSKSTDFLSRHPEIANIFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINTTRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTTASAGLY
                     GDAHAGLDAKPSLFGVRVDVGAKVGGRASIDGSDLDSHEASSGSRASHDARHDIDARA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIHGEENIVGRQYSELQNHHKTEALSQNNKYDEERES
                     QKIIPEPSGEAVKEMIDGKKERLKGPL"
     gene            complement(3101..4474)
                     /locus_tag="OHN28_002954"
     CDS             complement(3101..4474)
                     /locus_tag="OHN28_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCQ2298306.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(4840..5181)
                     /gene="trbJ"
                     /locus_tag="OHN28_002955"
     CDS             complement(4840..5181)
                     /gene="trbJ"
                     /locus_tag="OHN28_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCQ2298307.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGLNELS
                     EISEGTYNERK"
     gene            complement(5111..5656)
                     /gene="trbB"
                     /locus_tag="OHN28_002956"
     CDS             complement(5111..5656)
                     /gene="trbB"
                     /locus_tag="OHN28_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCQ2298308.1"
                     /translation="MSLTKLLPVILLMMVTGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(5643..5927)
                     /gene="traQ"
                     /locus_tag="OHN28_002957"
     CDS             complement(5643..5927)
                     /gene="traQ"
                     /locus_tag="OHN28_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCQ2298309.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            6008..6283
                     /locus_tag="OHN28_002958"
     CDS             6008..6283
                     /locus_tag="OHN28_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HCQ2298310.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI"
     gene            complement(6285..6632)
                     /gene="trbA"
                     /locus_tag="OHN28_002959"
     CDS             complement(6285..6632)
                     /gene="trbA"
                     /locus_tag="OHN28_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HCQ2298311.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIMLSSVWS
                     GVMWFFIRGIMTG"
     gene            complement(6648..7391)
                     /gene="traF"
                     /locus_tag="OHN28_002960"
     CDS             complement(6648..7391)
                     /gene="traF"
                     /locus_tag="OHN28_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCQ2298312.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(7384..7641)
                     /gene="trbE"
                     /locus_tag="OHN28_002961"
     CDS             complement(7384..7641)
                     /gene="trbE"
                     /locus_tag="OHN28_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCQ2298313.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(7668..9476)
                     /gene="traN"
                     /locus_tag="OHN28_002962"
     CDS             complement(7668..9476)
                     /gene="traN"
                     /locus_tag="OHN28_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCQ2298314.1"
                     /translation="MKRILPLILTLVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCNSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(9473..10111)
                     /gene="trbC"
                     /locus_tag="OHN28_002963"
     CDS             complement(9473..10111)
                     /gene="trbC"
                     /locus_tag="OHN28_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCQ2298315.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPESRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQPSQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(10120..11112)
                     /locus_tag="OHN28_002964"
     CDS             complement(10120..11112)
                     /locus_tag="OHN28_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCQ2298316.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(11109..11741)
                     /gene="traW"
                     /locus_tag="OHN28_002965"
     CDS             complement(11109..11741)
                     /gene="traW"
                     /locus_tag="OHN28_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCQ2298317.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(11738..14749)
                     /gene="traC"
                     /locus_tag="OHN28_002966"
     CDS             complement(11738..14749)
                     /gene="traC"
                     /locus_tag="OHN28_002966"
                     /inference="COORDINATES: protein motif:HMM:NF010270.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCQ2298318.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVGMSSTQKPADVTAERRRHWWWTVPGCLAMVLLNAAVSYGI
                     VRLNAPVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHA
                     VILVSPAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(14909..15130)
                     /gene="traR"
                     /locus_tag="OHN28_002967"
     CDS             complement(14909..15130)
                     /gene="traR"
                     /locus_tag="OHN28_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCQ2298319.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PESRRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(15265..15780)
                     /gene="traV"
                     /locus_tag="OHN28_002968"
     CDS             complement(15265..15780)
                     /gene="traV"
                     /locus_tag="OHN28_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCQ2298320.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPL
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(15777..16028)
                     /gene="trbG"
                     /locus_tag="OHN28_002969"
     CDS             complement(15777..16028)
                     /gene="trbG"
                     /locus_tag="OHN28_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HCQ2298321.1"
                     /translation="MNKLVSDSSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(16040..16270)
                     /locus_tag="OHN28_002970"
     CDS             complement(16040..16270)
                     /locus_tag="OHN28_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCQ2298322.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(16224..16814)
                     /locus_tag="OHN28_002971"
     CDS             complement(16224..16814)
                     /locus_tag="OHN28_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCQ2298323.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(16804..18231)
                     /locus_tag="OHN28_002972"
     CDS             complement(16804..18231)
                     /locus_tag="OHN28_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCQ2298324.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNSPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(18231..18935)
                     /gene="traK"
                     /locus_tag="OHN28_002973"
     CDS             complement(18231..18935)
                     /gene="traK"
                     /locus_tag="OHN28_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCQ2298325.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMIRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(18946..19512)
                     /gene="traE"
                     /locus_tag="OHN28_002974"
     CDS             complement(18946..19512)
                     /gene="traE"
                     /locus_tag="OHN28_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCQ2298326.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(19534..19845)
                     /gene="traL"
                     /locus_tag="OHN28_002975"
     CDS             complement(19534..19845)
                     /gene="traL"
                     /locus_tag="OHN28_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCQ2298327.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(19860..20219)
                     /gene="traA"
                     /locus_tag="OHN28_002976"
     CDS             complement(19860..20219)
                     /gene="traA"
                     /locus_tag="OHN28_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCQ2298328.1"
                     /translation="MDAVLSVQGASAPIKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            complement(20253..20480)
                     /gene="traY"
                     /locus_tag="OHN28_002977"
     CDS             complement(20253..20480)
                     /gene="traY"
                     /locus_tag="OHN28_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCQ2298329.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
BASE COUNT         4824 a         5694 c         4899 g         5146 t
ORIGIN      
        1 tctgaatttt tcaggtatag caagatacaa catcattcca ttagtaatgg caacaaaaag
       61 gataaaaccc attacgccac taaaacatat atcaaatccg gcatctcgga tatcaacttt
      121 atctgccagc aaacgaagaa atgttggcca taataaaacc cataaaaaca catacccccc
      181 tggctttatg acctgtaata atggcgggat attaaccccc tctacagaga gtacatcctt
      241 tagtttctgt gtctccattt ttatatcata gtgagtgatc ataatggccc cttcagtcgt
      301 tcctttttac catcaatcat ttctttcaca gcctctcctg atggctcagg tattatcttt
      361 tgactttctc gctcttcatc atatttatta ttctgagata atgcttctgt tttatggtga
      421 ttttgtagtt cagaatactg tcttcctaca atattttctt cgccatgtat gctgctctga
      481 gtatccccga tattgcctcg gtattctgtg accatattat caacctgatg ttttacatca
      541 tttcggatat tactgtcctg cgttctttgc tcaataatgg cctgatgtcc ctgatggtca
      601 gcaataatat cctggctgcc accacccgaa ggtacgctct ccattccttt accaatatca
      661 ccacgggatt cacgccaggc attatcaaca ccaggctgca cctgttcctg cacaaaagac
      721 cacgccattg cccgacggcg ttctgcaatc tccggcgaac tggtatttgt cagaatagct
      781 tctgcatcct gcggagcgtg tttcatcaca tactgtgcaa actgttgcga taggtcttca
      841 ctcatctgcc cactcatgct ttcagtacgg gatgccattt cagcatattc atggctgcgg
      901 gtcatattcg tcgtgtactg gtcgtaactc tgttttgctg agttcagggc tgcagacagc
      961 tggtccacac gggaatcggc attattgtca gtatggctgc cggattcact gactttgcga
     1021 ctggtaaagt aatcgctggc ctctttaaag tccttactgg ctctagcgtc aatatcatgg
     1081 cgagcatcat gggaagccct gctgccacta ctggcttcat gtgagtccag atcactaccg
     1141 tcaatactgg ctcgcccacc aaccttagcg ccaacatcaa cacgcacacc aaaaaggctg
     1201 ggctttgcat ctaatccggc atgagcatca ccatacaatc cagctgatgc agtcgtactt
     1261 ctcgatgcca gttctcttgt cgcctgctca ttactgatat tatgcgcctt cgcatagctt
     1321 tccactgcac tgcgcatacg gctggccatc atggagtcct gtgcactcat cgtactgtca
     1381 gcgccaccgg tgacagaatc actgctcccc cggttagaac caaactgact gagcgtgttc
     1441 caggcactgg caatactgct gctgaaccca tgcagggcac tttctgctct gttcgatgct
     1501 tcccgggcca tttcctgttg tgccgccgca atctgacgcg ttgtattgat gccgaccggt
     1561 aaccgggaca tcgccccgct cgcatccatc accatattac cgtcacgggt ctgtgtggca
     1621 gttgcgccac tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc
     1681 cagctgaagc cgttcacgtt ttccgtctgc atgttgccgt aggagtaatt accgtcaacc
     1741 acactacctg ccgcactggc agtcgggctg atagcagagg aagcgaaatg gctgtacaca
     1801 ctggaaaatc cggccccgag tcccctgacc atcatccagg ataatggcgg gatcatcatg
     1861 gaaagatacc cggcagtgga ggccagatcg gagtatttca gctgtatctg tgaaatttcc
     1921 gagagcacca ccggtgcacc attctgcttc gcataaaatg tcatggcact gttcagaatg
     1981 gcgtacaaca ttggccatga ctgcagccac atcagggcaa acacatagcc tttcaggacg
     2041 gacagcgtca gtttattgaa gacagccgcc agtaccagga gcgggaaaat accaatggcg
     2101 atccccgtca ggatggtctg cgtcatgggc agtgtccgca tcgctacatg gccgatggag
     2161 acatgtgcca gacgttgctt ctccatcgat gaggtggtgg ccagattcac caggctggcc
     2221 gaatcaccat tacgagcggc ataactggtg atcccctcct tcagggcatt gatggtaaca
     2281 ttctggcgga tgatctggct ggctgattta cttgagccat agaagtaact gtaactgtcg
     2341 ccaatcagcg tactgaacaa caggttcgga tcagggcgtc caccaaatag ctgctgggca
     2401 taataatgcc aggtttttcc gccactctgt gtgtccagat tcagcttgtc tttcagcgaa
     2461 accgacgcgt ccttacaggt tacgaaatta ttattgctgt cataaacgcc ccgcagcggg
     2521 ctggggcggg aaaaaatcag cgtgtaggga tcagcggagg ccatcagctc ctccagcgtg
     2581 tatttgtggt tcaggtaaat atcccccatc acgcagttct gcacataatc ctggaaaata
     2641 ttggcgattt ccggatgccg ggacaaaaag tcggtgcttt ttgataccat ttctgcgcca
     2701 aacagcatcc ccgttttgct gtaggtgaca ctgtccggtt gcgtgaagat catctcgtaa
     2761 ctggccacca ttgcatgccc gatgcgggtc gtcagtgaaa gtggcatcgc cagaccgacc
     2821 ggcacattat ccacccggtg aactttgacc aggtcactgt tatcaataat ctgcaccgat
     2881 gtgcggacat taaccagcag gctgataagc acaaacacgg ccacccagcc cagcaaatcc
     2941 atcacgttgt gccgctgtac ccacattacg gccaccgcga gaacagacaa tgtgagcgca
     3001 attttttcaa tggaatccca cgtcctggtt cccataaagg cggcaatggc gttcaggtta
     3061 ttccgcaacc actcaccacc ggcaatcaca taaacttcat tcacagcgtg ctccctccga
     3121 agtgatagtt gttctggtaa cgactgagca tgcgggcgga aagctgctga cgcatgtagc
     3181 tcatctgacg atcgacaacc agcagcgcat cctgctgtac ctgcacctgt gactgaaacg
     3241 ccgcaatctg ccgggtggca tcattcatgt tgtcgttaat atgcccgata accgcttcgt
     3301 cataatttcc cgtggccacc atcgcccggg cctgctgtat cagctcctga atgtactgca
     3361 gcaggatgtc gtaaccgata tagtccgtca gctggtaaat catactgttg gaaacaccga
     3421 gcatctgcgg gtcaaccagg tatttgaaga cggggatggt ggtactggaa ataaagcctt
     3481 tttccttgtc atccagaggc gtgtcactga cagccttgtt ctgaatgctg gccagaagtt
     3541 tagtaatctg agacttcagt gcattatccc ggctgatggt caccggtgtg tctgccacca
     3601 ctttcaggca tttatcagaa tcgttgcagt gataaatttt tgctgtgccg ccttccatca
     3661 tggcccggat aattgagcgg tcagtggttc tggctgacag cggtgtgatt tcgccgttgg
     3721 gaccaaacac cagtgagccg gtcagcgtca tcacaaactc tttcagctct ttgttgccgt
     3781 caaacattct gtttttggaa agcgcattcc acatgatgtt aatgtttttg gtcacccgct
     3841 ccttatcctt gtcgctggct ttatccctga cgctgtcaga tttaccgcca acggtacatc
     3901 cctgccggga agccgcccag tcagcaaaaa tattgctctc accggcaatg tcctgacaga
     3961 ctttttgctg ggacacctgc gtccggggga aaagcccgcc gataatcccc tgcgccgcct
     4021 ggcaggaact gaggttcatg ctgttaatgt cgcttgccat tttctgcagg aagtcttttg
     4081 cggttttgat ttccggcacc gttgtctgca gggcaaggtc aaaaaagtaa ccggcagcat
     4141 tactcataat ctgcttaaca aaacgctgca gctgctcacc attaataaaa ctgaacgaac
     4201 cgagataagc gtcgatgccg ccacatccgg cattgatatc cggcagcgtc atggaaatca
     4261 gctgaacatt tttgacctga gtacgggcat acagggagcc gccgtaagca tagccggcag
     4321 cctgcccctg ccagacgccc ggctgtgtgg tattggaggc gaagcccagc ttgttaaaga
     4381 actgattcat gtcgctgttc acatccgctg acgctgcagg cgtcaccgtc agtaaggcgg
     4441 cacaaagaat aaaaagaggc ttaatgcgaa gcattattgc tgccctccct taatgtgttc
     4501 tcccggcgca tacggcgttt caacaggaat atcccgatat ttccgtatat taattcgtac
     4561 attgggaaca gaaaaaatgt aacaagaaat ccggctgcaa gcgcatataa cgcatacgga
     4621 atgacatata tcaggatatt ccacatacaa atcgaattat ttaattacgg gtccgttttc
     4681 cacgaatcaa tacaggcatc aaaaataata ttcatagctg tccataaaac aattccggca
     4741 gtcaggaata ccagagacaa tccagcatac ctgtcagtaa ttctggaaat gtatttataa
     4801 tctcgttctg tacttctgat tttgagttca ggattatttt tattttctct cattataagt
     4861 tccctctgat atttccgata attcattcag accagtcctg cggctgtaat atatttcaga
     4921 caccgaaatc catcccacaa ttgcccccac aaaaaacaca acagaaacag aaaaggtaag
     4981 gcacagcgaa acgacaattt ctacagggct ggcgggataa aaaagcccct gatggataaa
     5041 ggggatattc aggctgacga ttattcccgt cgcgatgccg gatattccgg caatgagtaa
     5101 caccacctgc ttatttcgca ccttttttcc ctccgtacat ctgcagaacg gtatccatcc
     5161 gcgccataaa accggcggca tccgttgccc cctgtaaaag cggtaatgcc tcaagcgtgt
     5221 tgacgttgac caggaaggtg gtcggtgtgg ccaccggaat attcgggaag aaggtctgca
     5281 tcacgtccgg tggcaccggt aatgcttccg gaaaggccgt atccccctga ccatccaggg
     5341 tgtagggaaa aacggaaaag ccgtactgct gtgccagctg cttcagtacc gggtcaaact
     5401 ggtgacagta agggcaatgc ccctgcataa acagcaccac cttccagtcg gccaggttaa
     5461 cctgcctacc gttgctgagg cggaaccagc ggggagccgg cttttctgtc ctggctgatg
     5521 tgtctgctgc cggttgtgca ggctgcgcgg ccatgccctg cggattccag agtcgttcaa
     5581 tttcatcccg ggtggaggcc tgcacacctg tgaccatcat caacaaaatg accggcagta
     5641 atttagtgag agacatgtcc gccctcctgc tgctctgcca tcatggcctg ttgcctggct
     5701 tccagtgcct cacgctcttc ccggctgaca cgggcaatcc atgcatccag cacctggtat
     5761 gcgccgaaca gcacaaggat ggccgcaatc agctccgcca gaaaaaaagc catccagggt
     5821 ttgctgtaaa caagacgggc gacgatatga aaccagacac ccagggaaaa tacccacatt
     5881 cccgtaatat caagccgggg taaagagaat ctgcgtttac ttatcatgtt aaactccggt
     5941 tattatttac atgagagcat tcatgtctgt aatattcgcg tttgtgtgat attaatcaga
     6001 ggtacaaatg gaaaagcgat cttttaaaga aaaactggaa ataatcagaa acataataag
     6061 agagtcgtta ctgggaaatg cggcaattat tgcgctgata tatgcagcat cacattcatt
     6121 accggtcaat gccttccctg actatcttgt aataagttta ttaagtatct atatttattt
     6181 ttgtgagcta ttcagaagcc actggattgc ggtatggttt atcatatggt cttcagtaat
     6241 caacctcatt attttgtatg gtttttatga ccgttttata tgacttagcc tgtcataatc
     6301 ccccttataa aaaaccacat cactccggac cataccgatg acaacataat acagataaat
     6361 attcttctga acctgtcgaa gctcgtaacg tccggtaatg cggccgcaac tattgaaaca
     6421 accagtgctg cgccctgact cataattgca ccacacagaa accagagact ggcggtaagc
     6481 acatataaac acgcgatatt aataacaagg aaaaccagtg agctcaggag aaacattttc
     6541 ctgtcattac gctcagaaaa gaaataaccg gcaataatga caaatattaa cagaacaaca
     6601 cctgtaaaca ttttcaaata atcttcactc ataaatcaaa tccgcgatta aaaattgggt
     6661 ttaaaatctt cagaaacgtt caggaactgt tttgccaggt cgtcctgcga aataaagccg
     6721 tatgataacg ggcgaacact gccctgtttc gggtcaacca gcatcatggc cgggaaatat
     6781 ttcacgccga ggcgctgcgc ctgcccctgg tcagtccggg aatccggcaa cagcggatta
     6841 atcacgccat ccacggaaac gggaataaca ctcagaccat acgtatcccg gaagccatta
     6901 atgacctgcg ccagttgccc gtcgatgggg tcctgccccc ggtaaaaaaa catgatgccg
     6961 tagtgttcag ccagtttcgc aatggcctgt cgctgctgcg cctggtctgc tgccagctgg
     7021 ttccgtaccg tgccgttgta atggctgtac tgcaggttat agtccagttc aggatgtgcc
     7081 agcatggcct ttctggcgct catggtgaaa agcccggcct gctgagtcca gtaattttgc
     7141 agccggaaat atttcacaaa attatccacg ccgggataca gaatggcttc gtacagcgcc
     7201 cgctttgttg ccgtctgcag tgctgccagt ttctgcataa tatccggttc ctgacggggg
     7261 gctgcaggta caggtttatt ttctttttcc ttcggattta ttttctcgtt ataccactgc
     7321 cagcctgcat cttttcctga cgccggaaaa ataaagcagc agagtaacag tggcagtaat
     7381 gctttattca tcttcattcc tttcatcact ttttctttca tcaggtttct gcaggtcagt
     7441 cagggcagaa aaacaaaaat acagaataaa caggagcaca ccggagtaaa ggataacgaa
     7501 agcagcggca agcatcccgt ctgccgtggt ttcttttacc gtatcccacg accagataat
     7561 taccggggaa atgacaatcg ctgtaatcaa aagccggata agaaaatcaa taaaacggtt
     7621 cgacgtgaat atgactttca tcatttcccc cggttatttc ccatacctta ctgtcctgcc
     7681 tgtttcagct ggtcagcaat ctgctctttc actttctgcg tcagaacgcc actgtccggg
     7741 attttctggt tattcatcag gtcttcgtag aagttagtga aatccagtct gttgaactga
     7801 attttctgca gctcatcaac cgtaatcccc cgacagtcag gatgcttcgc actgccaaaa
     7861 ctgatacgca gctgtccgtt gcgcccctgc tgctggacta tctgcgccag cttcgaatca
     7921 aactgacagt agctgcgttt tttctccaga cagacaccca gcactttctt cgaacagaac
     7981 tccccgacgc tgacggtcag tttgtttgat tttgctttag ccagggcctt ttcatcactg
     8041 ttgcatttgg ccagcccgat atcctgtccc cagccgctgt ccttacagca gttgctgtat
     8101 ccggctgcag ccttcttaca aaatttcgcc tgaccggtga aagcacggac atccaccccg
     8161 ttcagcgccg caacatcctt acctgctgcg gccagtgccg ccagctgaga aacggcttcg
     8221 gcaaaatcat tactctgccc gctctgagcc ttatcgcatt cgccatcgag acagaagacg
     8281 tctcccccac agaccatcac tttcccggat gtttttgact cacaggagta cgtggcatat
     8341 tcatgcagac aggcaccttc ttcggagtaa aaggcacact ggtgtgacac cagggtacag
     8401 gcaggattgt cggtgtaggt ctgacaggtt cccttgtctg ctgactgcgt gacatacgtg
     8461 tcccggtatg cccagcagct ctggtacatg ctgtatgact gaccgtcttt caccagcgtc
     8521 ctgttcccgc cagcctccgt acattctgtt tttgtcagtt tccccagctc cttgctgaac
     8581 gggcagcttt cagaccagac aacatacggg gtgtacgagg ctttttttac cctgacaacc
     8641 agacgcagct tcattgccgt atgacgtttg aattttgtat tcccgacccg gacggggtga
     8701 gatgacgtaa aagccacacc ggcagtcagt gttttctgta ctggtgtaaa tgaggcatct
     8761 gctgagtatt tggcattcca gctcagtggg gttcccagta ctgtaattgt cataggtaca
     8821 ctcttctgca ggtaagtacg actccagctg taatgcgcat cgacaatttc accggtaacg
     8881 ggagaggtaa tggagacaac atactgacca ttcacttcac gtgcaggtag ctgactcatt
     8941 tcatactcca gcgtccgggt ttcccatgtg gtactcccct gcagctccat acgggcagtt
     9001 cgtgtgcagt actgctccac ctgtaaatcc cgctcacagg tgtaattcgt gtattcactc
     9061 ctgctaatct cctgcgcact gcactgctgc ccggtgtttc ccacaatgct gtcagcccgg
     9121 ttcaccacat ccctgccggt ctggataaac ggtgcatccg gtgaaagaat gtctttcggc
     9181 ttgttcatga atgactctgt aatggttttt ccggtttcac cggtcgccca ttccgtggtg
     9241 ccgtcatttt tcaggccgcc atccccgccg gcagtcacgc caccgtaata ttttgtctcg
     9301 tccggattcg cgttatagcc ggggatactc tcctgtggct taaagccctg aatactgctg
     9361 cttccctgcc ctttgatctg gtgagcaaaa tcagaaccag cccggtaatc actgttgctg
     9421 tcagcctgtg ccatgccggc aaccagagtc agtatcagag gtaaaatacg tttcatttcc
     9481 cggaatctcc tttccctgcc agtaaatcat gagccacctg ccggcagtca cccgtggcgg
     9541 ccactttctc cagcgcctgg ccgacccgca ggtttccccg gatgatgtcg tatccctgac
     9601 tgcagaacac caccagtgcc ggtacagtgc gaatgccgta ctgtgaaaac agcgtcgggt
     9661 cgatctgaac accatcagtc gcgccgtctt tcaccaggga caacacagct tcagcggtgg
     9721 tcttcaggtc attgttcacc atgccccgca gtgtggccgg aataccgtag tgccgggttt
     9781 cgcccagcat tcgtttcagc ccctcttcgg gaatggaaaa cgacacaaaa tacagagcac
     9841 cctgccgggg tttcccgtcc tgcgaaggct gctgtttacg aaccagctca tccaggaaat
     9901 ggttatctga acgctgaagg gggttttcca gcacctgttt ctccgcccag gctttcagct
     9961 gatggtcagg tttttcacgc agttgtctgc ttaaattttc ctgctgcttc aggaactggc
    10021 ggctttcagg agtgttcacg ttttctgacg ccataaccgc cccgttcagc atcatcagca
    10081 gtgctgccag agatttcata ctcagcttca tcactcacct cacaggaaga cacagttacg
    10141 tttacgccac atcaggtagc cgaagttttt ctttgtgttg ggcggatttt tcccggtttc
    10201 ccagcgggtc acgctgcgcc cgaacgggtg gcactgcccg ctgtccggat acatattcac
    10261 catctggtaa cgccagcgtt ctttgggcag gattggggac ggatattcat tacagacggc
    10321 gttatttttc ccgatggttt ccataatcat gccctgacgg tgcagcttga acgccatgcg
    10381 ttcactgacc agcagggagg actgcaacgg actggactca ttactcaccc agccattgaa
    10441 cgggtacata cttccctgcg aaccggcaca ccagaacaga acatcgagag gcatattaaa
    10501 ggcgctggca atcgcatctg ctgcgcaggc tccctgtgct atcggattgg caaagatgac
    10561 agcttccgga ttgagaatgg tggtcaggct gctgtccgtc caggtggggt cgatttcaga
    10621 aagataagcg atatccaggt caccaccttc cagacagccc agcgatgtga tgatgttcag
    10681 ccagtacgtc agcgggtatt tgtaccagtg aacgtgatag aacgccccat ttacctgctt
    10741 ctcatccttt ttcgccgttc cctgtgccgt tttaccaaaa gccggcaggc tgaagcccag
    10801 gttcaccatg catcccggtg accgggtgac gtccgtcaac gccatcggct cccagtaacc
    10861 aatggccagc ccgatacgcc tgaacagcgg cggcggtgcc ggacaaatct gaatgggcat
    10921 cgacgggttc gccgtgtcgg ggaccttacc ctgactgact ttgatactgc ccagcgagag
    10981 cgggaaaata cagctccagc agatatctgt gatcgggttt acaaaacgcc cctcacaggc
    11041 agaatccgca gacgccgcag ggacatgacc ggcgaaaagg aataacatca gcagccacag
    11101 ccttcgcttc attttctgcc ctcctctgcc ggaataaatt ccaccttcag gaagcgatcg
    11161 ccgggaacgg cgctcacccg tgccgggacc tgatcaatgc cgaggcgctg gcagagcaca
    11221 ccgttctggt caaagtaaac acggctgtcc agagacttct gcatctccgg gatactgccc
    11281 tgcacgagga taattttgct ctccagtgtg ggcggcgtct ggcgtttcat ccaggccacc
    11341 tgcgccggat catcgccatt gatgaaatac agcgtctggt taaaaggaac atactgcagc
    11401 ggattcatca cttcaccctg acgggcaaac acccgccctt cgttatcccg gatatccgca
    11461 gccagtctga cggacgggtc aaacaaccgg ctgccgtatt tctccgtgcg tccgataccg
    11521 ggcacggcag ggggccgcag actgttgcgg atcacccgct ccttaaaggc atccattttc
    11581 cggcccatct caccggactg ctccagtgcc gtcaggcgct gcatgatcac cgtcagcatg
    11641 tccggctcct ttaccggcca cagatcgccc caggtaccaa gatcggcggc ggccacactc
    11701 tgcccccata tcagcagggc aatcagcccc cgacatctca tggttccgcc ctcattcgcc
    11761 gggcaatatc ctgctggatt tcccgggtga tatccggtgc cccctgtacc acagcaggcg
    11821 acaccagaat gaccgcgtgg tgtttctgct gccacgcctg caggctggct tccagtgccg
    11881 tgttaaaacg cgctgaaagg gcttttgact gcgcttctga cagttgtttc tgactggcgc
    11941 tgtcaaaaaa cgcatccacg gtctgtttca tgttgaaggc gacagtcacc ggtgcgttca
    12001 gtctgacaat gccgtaactg acggctgcgt tcagtaacac cattgccagg catcccggca
    12061 ccgtccacca ccagtggcgg cgtcgttctg ccgtgacgtc ggcgggtttc tgcgttgaac
    12121 tcatgcccac actcctgtat ttctcatgtt cttccagcca ggcttccagc gaagccattt
    12181 ccggccctga cttcttccac gccagctgcc acactgcctc atgaatactc agcccctctt
    12241 tacgcttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg gcgcggctta
    12301 acgggtccac aaacagacgg tgccaggagg aatggttttc cacctgcagc aggaaggaac
    12361 tgaaccactg gtctttggcc gcaccaaact taccaatcat gtcgcgctgc agtggcagga
    12421 actggtccgg atacagttgg ttgtatttcg cgaactcctt cgcactctgt ttgaggataa
    12481 ttttgtagga ggagttaccc catgccgcgc gggcagcact ggaggcctta tcagagtcaa
    12541 agtcgacgat gttctgtgtg atggtgatat aggcaccggt atgacggcgg gccgtacggt
    12601 agcctttctc aataaattca ccgaccttgt gatttttgaa gtccagcaga cgccagcctt
    12661 catcaataac gttcagtttc ttgaggttac gcggcgtgcg gtacatcctg ttctcgatgt
    12721 agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc agtccgccca
    12781 gctccagcac caccattttt gcgtcatccc gcagggacgg ctcatcagag ttaaaatact
    12841 ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg tccagacggc
    12901 tgcggatagt cggtgactcg gcatactgct cgctgtcact ggcgtttttc aggaaatcca
    12961 ccacgtcatc aatacgtgct ctgttctctt tggccagcca ggaggctctg accgcctgca
    13021 acagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc actgacaact
    13081 ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac gggttaaaac
    13141 gcagggtttc accgtccaga tacaccccgc ccatgttctc acacagggac ttgtagccat
    13201 cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg ataagtggct
    13261 gtatcagccc ggttttaccg gccccggagg tgccacagac cgccatgttg tagttggtgt
    13321 tattcatccc ccggaagaaa atatcgataa acgccagctg gttacggtag gtgggtgcca
    13381 gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg gccacattaa
    13441 agctctctgc gcgctgtacc actccggcct ctttcagctg tttaaacaac cctttcccgg
    13501 ccataaaggg cagacaggtc aggaaattgc gcatgtggtt aaagcgcggt gaaatcagct
    13561 caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagc gccgtttcat
    13621 tattgtcctt gcagaaggct gtgatgttga ggaagtagga cacgacagag gactgaccgg
    13681 agcccagccg ctgacgcagt tccccccact ccttcgcctc tttctccacg gacggaaacc
    13741 atttggcata ggaggtcttc gactttttct ccaggtccat gtacttcagg ttggcttcgc
    13801 tgtgagtttt cacctggtct tccaccacca gagtcagcgt caggatgaac ggacaggaga
    13861 tggacagttc cgggttcagc aggttgctgt agttgtcggc cacgttccac aggaaggcga
    13921 tttccgggtt acgggccaga tggaaattca ggatgcgggc cgtgctgttc ctgccgttct
    13981 cacgcaggcc cagcgtcagg taatcagccc ggactttcag atcaaaactg tcctccacac
    14041 actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac agggagtccg
    14101 ggttatggtt aatcatctcc ccgacaatat cgataaaggc ctgtgcatcc accgtctgtg
    14161 tggtgatact ggccccctgt aacgacgccc ggatgatttt caccaggttt tccatttcca
    14221 gaatgtcggc ccggcttttt ttcttcgaag gagaacagta cgagataaac acccggtaat
    14281 ggcgcagggt caggggcaga ttcatcccct ccggcagcgg aaactgtgtc gctgccgctt
    14341 tcatataata ggcccgggta atggcgttaa accgttcagc ctgttcacct gaccaggaga
    14401 actcacgcag cccgtattca atcctgtcac caaccagctg actggacatc agatggatac
    14461 acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagggcc tccacaatgg
    14521 actcattcgc cccattgatg gggatggctt ccagcataaa gcccatcgtg gtgtcattca
    14581 tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg aactgcggga
    14641 agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt ttcagtgctg
    14701 tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattcaca tgaattcctt
    14761 agttgaaaaa taaagcgaga aatgcggtag ccatgaaggg aatacctgta attaacgcgc
    14821 catttcccgg cgcatataca aagatttctg ttttctgaat atccactcat gttcatccgg
    14881 aattaaatca ctatgttctg cgactgactt atgcataatg ttttctctgt ctttcctgat
    14941 acgcctgaca ttcaacgcac aacgtcacac cgggaaatat tttccgccgg gattccggaa
    15001 taggatttcc gcatgcttca cagagataaa caggaatacc atgagcattt attttctggc
    15061 gtatccggtt tattcctgtc atggtcagtt gttctgtcac tgaatatgct tcatcggctt
    15121 catcactcac ggaatattcc tcccgacatg ttctgacacg ctaccgccca tcccttttca
    15181 cgcacagggc actcgtttta caggcagatt ttgaatacag aaagcccgcc ggcattgccg
    15241 gctgatatac aacttcaggg ccagttaatt aatacgtggt tttccccacg cagacggttt
    15301 aataacaaaa aacacgctgg atggctgatg ataaacatcc tggttatcaa tataaggcgc
    15361 tatccataat gctgccgtct gctcacctgt tcttagcgga cgaggaggtg ttaccggcgt
    15421 aactgaactg accggaacaa ccgttttcac ttcccgggca gcggtaaaca gcgggagagg
    15481 tgccagcagt ttctgctccg gatgtgctgt caccgcaggt ctgctgccgg acggagtggt
    15541 cgcggtgacg gtctgcaccg gcattgtccg gaagttccct tcagccaggc gcggcagtga
    15601 tgccgcaacc ggctttgctt ctgaggactg ctccagtttt ttggccttct cattggcctg
    15661 ctccatcgtc atacaggtat cggatgtggt ggcgttacac tcaaattccg tactggttcc
    15721 ggcacagccg gaaagtaata aggtccccag cagaggaata aagaaagaaa tctgtttcat
    15781 tgtccctcac ctttctcatc gcagttcaca aaatccttct tcaaatccct gaccgtacag
    15841 gtttttacaa tgtcaggatt ttcaaaaacc gtactccgtg atgatttata aacatatgcc
    15901 acaatgcgtt tcccacccgc atcaggttct ggtgcactga cttcacataa cggaggtgtt
    15961 attccggact catacagcac cggataattt atttttttga cgctgctatc agaaaccagc
    16021 ttattcatcg aagggttcct tatatttctg aattcttcat cacaccgaca gcagtcatat
    16081 taaaattgtc cggcaatgac tcagaaccga atttctccgg tgcaggaagt acgattcttg
    16141 tggcgcagtt actgagaaca gcatgcataa aatcatctga cacggtttta cccggcacag
    16201 agagggctgt aataacatta atattacgca tgttcatttt tatctccgtt gtttctgttt
    16261 gttctgtcat caggaagata aacagggcat ttcctgattt ttcccggcag ggaagcgtct
    16321 tccgtataaa cttccttcag atgttcatct gcggttacca cccggataca tttcattttt
    16381 ccgggtcgca ccggatattt cccggtgatc gctgccagcg agacatacat cagtgccagt
    16441 gttgcccata atgcggtgac cagttgcatc agtgaccggt cagtctccct gacataatct
    16501 gcagcatcag ccgcgcggta atggcagata tcctgtattt ctgatctgcc tgaagaatca
    16561 gaggaagaaa accagcattc actgaccggg aactgtccgg caggtgctgt ttgtcttacg
    16621 aagttaatct ccttaaccag taatttcccc ggcgtggtgt tgtccttcca taaaacaaac
    16681 agagccatca gggccattgt ggccagtggc agaatgacgg tgtacttcag acaccagaac
    16741 agtcctcgca gaacccgggc gacaacatac cgggcggcat ggcacgcctg acgggacgac
    16801 atattatttg ccatcgttgc cccctccctg gctgccggac ataaactgct gcggattcag
    16861 gttgcccagc tgcttcagca tctggtcagt attaaagccg ttaagatgac tttcagccga
    16921 tggcggaaca ggaaccggac tttccggatt gtcttcttcc gctctgttct gcgtgcgctc
    16981 cagcgccatc tcttccacgg ttttcagctg gaagccgtcc tggaacacca cggtcacttc
    17041 gttgcccgca ccaatcggta tcaccgggtg atactgttcg gcacgtttga tgtagtagtc
    17101 actgagcgtc tgtgcggctt tcgatgcgcc gccaccgata cccattttaa ggacatcgcc
    17161 ggccccgtaa gcagcagtgg cacccagccc gacagccggc tgggaagcac gctccattcc
    17221 ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag ccgaggattt tgccgttacg
    17281 catcaccact tcgcccttga taccgttttt accccggaag ctgacatgcc ccttaatcgg
    17341 catatcaatg gttttgccgt ccttcaggca actgatattg cgggtgcgta caatggcacg
    17401 ctcactggac acatcccccc aggcttccag ccccacaaaa catcccgttg cgtcatacgt
    17461 tttgctgttc ggcatttcca ccaggccggt gatacgcagc tgcatcggca ccgtggattc
    17521 attaccggtg actgaggcat tggcatccgc cccttcaatc agcatggctt tcgcaaaact
    17581 tcctgacgga atgtacggca gcgatggtcc ctgttttccc tcattacggg taaacacctt
    17641 acgctgtatc cggttaggca ccggtacaga ctggtacgtc acctgcggtg gtggcgtgac
    17701 accattcccg ggataaaacg ccgtcggcgg tggaacagca acgctgccct gcggcgggaa
    17761 ggatacgggg ctgtttcctg gctgtggttc gccttccggg ccgggcggtg aggctggcgt
    17821 ctgcggtaca ggctcaccgg tcgcggtgac gggatttgca cccagggctt ttacctgctc
    17881 tgccagggca gcattgtcct gtcccagctt ttcaatacga cgctggtcat caccgcgctg
    17941 tttgttcagg acatccagtt cacgacggat ttcctcatac tgcttctgca tctgtgctgc
    18001 cgtcacctgc atctctgtgg tggcatgctg acgcaccttg tcatcaaagg tcgtatccac
    18061 cacaccggtc atatccggca caggctcctg ttcagccacg gtttcaccgt taccggacat
    18121 gtccacatca gacagataca gcgcaccacc aatcgcggag gctgcaccga caaccacaat
    18181 ccccagccac aggtactgct tgcgtttcac aatggtattg atactggcca tcagttgccc
    18241 tccccgtcac ggatgacata cacatccatg cgcgcacctg ccagtaactg gctggcaggc
    18301 tggctgaaca tcacggcacg ggttcccggc tgccagaagt cactttcccg gatattcagg
    18361 gcagacagcg ttttattttc cacggcaaag cggatcatct tcagatgatt ccccgtccat
    18421 acagcatctg ccactgaaga cagccccgcc ggggcctgca gggtttcttt tgtcaccggg
    18481 acctgatacc agcctgcagg taattttccg ccacggacgg cctggctgat ggtcacaagc
    18541 agggattcgt aaggcgtgga cgtttcccac gcaccggctt cttctccggt tccgcgcagg
    18601 tcactgacca gctgaatggt acgccccgcg ccttcacggg gaacggcctg aatggaaaga
    18661 ttcagaccac gttctgtttc cagaatgaac gtaaagggct ttttgttgac ggtggccacc
    18721 accacaccgc cggaggcggt ctgctcatta ttggtcaggg caccatccag actgttcacg
    18781 gcgataatcc ggtcaccggg aacggtaaac agattcgggc tggtattgct gatggcaata
    18841 ctggcctgac caccgttcac cattggcacc acggtggggg ccagcgtacc gtttgcggcc
    18901 atcacgctgc aggaaaaaaa caggctgccg aatattattg ccgtattatt ttttctcatc
    18961 gtctgtttcc ccgaaattat ccagccaggt caccccgttt tcccgcttca gaataaggat
    19021 gtaatgtttg atatcagtga aaggttttga atcaccaatc caggttttaa gcacaccacg
    19081 aatttccaca cggccatact gaggccagac acgaacactg gtctggaaaa aggctgagtt
    19141 cacgttatcg tttttaatac gcttcgcttc ttcagccaga ataactttca tctggttctg
    19201 tgcgcccggg cggatatatt gcagaagcgc ctgatgtgag gcatcgacgg tttctgatga
    19261 aacattcaga cggagggcaa taaatgacag cgccatctgc tgtaaataag aggcatcggc
    19321 actgttctgc gacacggcaa agggggcatt aaatgccatt ggcgtcacgg cagtgcgttg
    19381 ctcattctga agacggtagt tattcacccc ctgaatgacg ttaacagaga ggctgagaac
    19441 aataagcact gacataaata taaaggcgat ggccattaca cgactggtac ttaaacgggc
    19501 accgtgttcc atataacaat cctggtatca gttctattta atccactgcc ggaaacacga
    19561 atcgggaaca ttatgaaaaa taccgcgcag cagggctgtt ggcatatacc agtaaatcag
    19621 gtcacgtaac caggaactgc cccgcccttt tttcagtttt ttaatcccga aataaaccag
    19681 aaccgctgca ccaataccga acagatattt cgatgttgtg ataccccagc caatacagat
    19741 tgctgcgggg atcagttcat ccagtggcag gccaaaccag cggctctggt tggtcagtgt
    19801 ttccgggaaa cgatatttct taagtttatt ctcgtctccc gacatcgttt tatttcctgt
    19861 cagaggccaa cgacggccat acccacagca ataaatacag agatgatggc aaaaccggcc
    19921 aggaacttga cgtttttggt catcatgtac atgaccgcac cgaccagaac ttcagccaga
    19981 acgacccatt taacaacact ggagtcctta ccgaaggtcg ccttaaccgt ggtgttaccg
    20041 cttgccatca ggtcctggcc ctgtgctgcc attgccagtt gcgggaagaa catcatcaga
    20101 acagcagccg ggatcactgc gcgagccagg cgaagcatat tcagacgagt gaatttagaa
    20161 aaaaacgact tctttttgat gggcgcagaa gcaccctgaa cacttaaaac ggcgtccata
    20221 tgattcctct gtttaatatt gatagataaa aactaaagtt ctttaaatgt tgactcgctc
    20281 tcttcggtca cctccctgaa tatttcttcg ttatagaaat caggaaatcg tttcagatga
    20341 tctctgagac ggatctggac ttctatcgtt ttacttcttc cactacgatc tttggcctta
    20401 ataagtcggt tatttgtatc ttcatcgaga tacaccgaga ccgttctgct tataccgcct
    20461 ctcgcgttac gcctcctcac tcaattcacc tctgtttccc gactaataaa attatgattg
    20521 cacagaaacc gacgaaaaaa aacacgccaa aattggcgtg tct
//
LOCUS       DAJOTB010000084        20261 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJOTB010000084 DAJOTB010000000
VERSION     DAJOTB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.084"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..273)
                     /gene="emrE"
                     /locus_tag="OHN28_002978"
     CDS             complement(<1..273)
                     /gene="emrE"
                     /locus_tag="OHN28_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCQ2298330.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGM"
     gene            complement(617..931)
                     /gene="fliE"
                     /locus_tag="OHN28_002979"
     CDS             complement(617..931)
                     /gene="fliE"
                     /locus_tag="OHN28_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCQ2298331.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1146..2804
                     /gene="fliF"
                     /locus_tag="OHN28_002980"
     CDS             1146..2804
                     /gene="fliF"
                     /locus_tag="OHN28_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCQ2298332.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEMEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2797..3792
                     /gene="fliG"
                     /locus_tag="OHN28_002981"
     CDS             2797..3792
                     /gene="fliG"
                     /locus_tag="OHN28_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCQ2298333.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3785..4471
                     /gene="fliH"
                     /locus_tag="OHN28_002982"
     CDS             3785..4471
                     /gene="fliH"
                     /locus_tag="OHN28_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCQ2298334.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4471..5844
                     /gene="fliI"
                     /locus_tag="OHN28_002983"
     CDS             4471..5844
                     /gene="fliI"
                     /locus_tag="OHN28_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCQ2298335.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSKTHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5863..6306
                     /gene="fliJ"
                     /locus_tag="OHN28_002984"
     CDS             5863..6306
                     /gene="fliJ"
                     /locus_tag="OHN28_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCQ2298336.1"
                     /translation="MAEHGALAPLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6303..7430
                     /gene="fliK"
                     /locus_tag="OHN28_002985"
     CDS             6303..7430
                     /gene="fliK"
                     /locus_tag="OHN28_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCQ2298337.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7535..7999
                     /gene="fliL"
                     /locus_tag="OHN28_002986"
     CDS             7535..7999
                     /gene="fliL"
                     /locus_tag="OHN28_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCQ2298338.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8004..9008
                     /gene="fliM"
                     /locus_tag="OHN28_002987"
     CDS             8004..9008
                     /gene="fliM"
                     /locus_tag="OHN28_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCQ2298339.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9005..9418
                     /gene="fliN"
                     /locus_tag="OHN28_002988"
     CDS             9005..9418
                     /gene="fliN"
                     /locus_tag="OHN28_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCQ2298340.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9421..9786
                     /gene="fliO"
                     /locus_tag="OHN28_002989"
     CDS             9421..9786
                     /gene="fliO"
                     /locus_tag="OHN28_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCQ2298341.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9786..10523
                     /gene="fliP"
                     /locus_tag="OHN28_002990"
     CDS             9786..10523
                     /gene="fliP"
                     /locus_tag="OHN28_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCQ2298342.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10533..10802
                     /gene="fliQ"
                     /locus_tag="OHN28_002991"
     CDS             10533..10802
                     /gene="fliQ"
                     /locus_tag="OHN28_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCQ2298343.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10811..11596
                     /gene="fliR"
                     /locus_tag="OHN28_002992"
     CDS             10811..11596
                     /gene="fliR"
                     /locus_tag="OHN28_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCQ2298344.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11886..12509
                     /gene="rcsA"
                     /locus_tag="OHN28_002993"
     CDS             11886..12509
                     /gene="rcsA"
                     /locus_tag="OHN28_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCQ2298345.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12553..12741)
                     /gene="dsrB"
                     /locus_tag="OHN28_002994"
     CDS             complement(12553..12741)
                     /gene="dsrB"
                     /locus_tag="OHN28_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCQ2298346.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12904..13131
                     /gene="yodD"
                     /locus_tag="OHN28_002995"
     CDS             12904..13131
                     /gene="yodD"
                     /locus_tag="OHN28_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCQ2298347.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13427..14242
                     /locus_tag="OHN28_002996"
     CDS             13427..14242
                     /locus_tag="OHN28_002996"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCQ2298348.1"
                     /translation="MFSIQQPLLVFSDLDSTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWITATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(14239..15933)
                     /gene="yedQ"
                     /locus_tag="OHN28_002997"
     CDS             complement(14239..15933)
                     /gene="yedQ"
                     /locus_tag="OHN28_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCQ2298349.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLKNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16104..16286)
                     /locus_tag="OHN28_002998"
     CDS             complement(16104..16286)
                     /locus_tag="OHN28_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCQ2298350.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16365..17282)
                     /locus_tag="OHN28_002999"
     CDS             complement(16365..17282)
                     /locus_tag="OHN28_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCQ2298351.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17455..18375
                     /gene="yedA"
                     /locus_tag="OHN28_003000"
     CDS             17455..18375
                     /gene="yedA"
                     /locus_tag="OHN28_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCQ2298352.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18364..18834)
                     /gene="vsr"
                     /locus_tag="OHN28_003001"
     CDS             complement(18364..18834)
                     /gene="vsr"
                     /locus_tag="OHN28_003001"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCQ2298353.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18815..20233)
                     /gene="dcm"
                     /locus_tag="OHN28_003002"
     CDS             complement(18815..20233)
                     /gene="dcm"
                     /locus_tag="OHN28_003002"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCQ2298354.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
BASE COUNT         4917 a         5358 c         5244 g         4742 t
ORIGIN      
        1 catgcctatg atagctggca ggtccagtcg ttgaccaaaa aatccccagg aaagcaaact
       61 aatcagaacg ataccgactc ctgaccagat agcataagca atccctgtag gtatataagc
      121 cagcgtctga gctaataacc agaatgatgc acaatagcaa ataattgtgc caacagaggg
      181 ccataaccgt gtaaacccct ctgaaaactt cattaaggtt gtaccaatga cctctgcaag
      241 tattgcgcca ccaagataaa tataagggtt catagcatat tctttcctgt tcatactgga
      301 gagaattgta ctacagtttg aacacaactc actgttttca tcctggtgta tgtgtatcca
      361 gggggctctg gcgtggcaag agataaaggc aggaacaata atgatcagaa ggaaccccag
      421 gctaacgaaa gagttcactc ttcctgggag caaagcgaag acaaaacatg ttatcgcgtt
      481 tacggatcgt ggatggcaga aaacaaccag gatcaggtat ccattttaaa ccagaaattg
      541 agtgagtttg cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt
      601 caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct
      661 tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca
      721 catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcgg
      781 ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg
      841 tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga
      901 taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctgatagca
      961 aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa acgggtttat
     1021 tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc gagtttgctg
     1081 acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca gccacgaggt
     1141 gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat cgcctgcgtg
     1201 cgaatccgaa aattccattg attgttgccg gttccgcagc agtggcggtc atggtcgcac
     1261 tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt tccgatcagg
     1321 atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc ttcagcgaag
     1381 ccagcggcgc tattgaggtt ccggcagata aagttcacga actgcgcctg cgcctggcgc
     1441 aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag gaaaagtttg
     1501 gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc gagctttctc
     1561 gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca atgccgaaac
     1621 cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta aatctgttac
     1681 ccggccgcgc actcgatgaa gggcaaatta gcgctattgt acatctggtt tccagcgcgg
     1741 ttgctggtct gccgccagga aacgtcacgc tggtggatca gggcgggcat ctgttaaccc
     1801 agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc agcgatgtcg
     1861 aaggccgtat tcagcggcgt attgaagcga tcctgtcgcc tattgttggt aacggtaata
     1921 ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg gaagaacagt
     1981 atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg
     2041 agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggcac
     2101 ctgcgaataa cgcaccaatc agcacgcctc cggcaaatca aaataaccgc cagcagcagg
     2161 cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc agtaactacg
     2221 aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag
     2281 tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct ctcagcaacg
     2341 aacagatgaa gcaaattgaa gctctgaccc gcgaggcgat gggcttttct gaaaaacgcg
     2401 gtgattcgct caatgttgtt aactcgccgt tcaatagcag tgacgaaagc ggcggagcac
     2461 tgccgttctg gcaacagcaa gtgtttatcg atcagttgct ggctgccggt cgctggttgc
     2521 tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc
     2581 gcgccgaggc ggtgaagact gtgcagcaac aagcgctggc ccgcgaggaa atggaagatg
     2641 cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac
     2701 tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg
     2761 tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac aggcactgat
     2821 aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac
     2881 ctctcccagc gcgaagtgca aacccttagc gctgcaatgg cgaacgtcac gcagatctcc
     2941 aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgccgca
     3001 ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa
     3061 cgtgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa
     3121 acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa
     3181 attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg
     3241 ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag
     3301 ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc
     3361 aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg
     3421 cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa
     3481 atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag
     3541 cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag
     3601 ccactgcgcg agaaattctt gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac
     3661 gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca gaaagcgatt
     3721 ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat
     3781 acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc
     3841 ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag
     3901 ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcagg
     3961 gattgccgaa ggtcgccagc aaggtcatga acagggctat caggaaggac tggcccaggg
     4021 gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca
     4081 gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg
     4141 cctgatgcag atggcgctgg aggcagcacg tcaggtcata ggtcagacgc caacggtgga
     4201 taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg
     4261 taaaccacag ctgcgcgtac acccggatga tctgcaacgt gttgatgata tgctcggcgc
     4321 aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccatc ctggcggttg
     4381 caaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact
     4441 ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc
     4501 acgctagata actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga
     4561 ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca
     4621 acctgtgtca ttgagcgcca gaacggcagc aaaacgcacg aagtagaaag cgaagtcgtc
     4681 ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc
     4741 ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg
     4801 ccgctcggtc cggcgttatt aggtcgtgtt ctggacggca gcggtaaacc gctcgatggc
     4861 ctcccctccc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccattg
     4921 caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt
     4981 accgtcgggc gcgggcagcg tatggggctg tttgccgggt ccggcgttgg taaaagtgtg
     5041 ctgttgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg tttgattggt
     5101 gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca
     5161 cgctcagtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc
     5221 gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt
     5281 atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa
     5341 ccacctgcta ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag
     5401 cgtgccggaa atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc
     5461 gagggcgatg accagcagga ccccattgcc gactccgcgc gggccatcct cgacggtcac
     5521 attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg
     5581 tcgatcagcc gcgcaatgac ggcgttgatc agtgagcaac attacgcacg agtgcgcacc
     5641 ttcaaacagc tgttgtcgag ttttcagcgt aaccgagatc tggttagcgt cggcgcgtat
     5701 gccaaaggca gcgatccgat gctcgacaaa gccatcgccc tgtggccgca gctggagggc
     5761 tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag
     5821 cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcaga acatggcgcg
     5881 ctggcgcccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg cctgctgggt
     5941 gaaatgcgtc gcggatgtca gcaggcggaa gaacagctca aaatgctgat cgattatcag
     6001 aatgaatatc gcaataacct caatagcgat atgagtgccg ggatgaccag caaccgctgg
     6061 atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca tcgccagcaa
     6121 cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga aaagaaacaa
     6181 cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact gcttgcagaa
     6241 aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat gaggaaacct
     6301 gaatgattcg cttagcgccc ttaattaccg ccgacgttga caccaccaca ttgcctggcg
     6361 gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca ttagcaggcg
     6421 agacaactac cgacaaagcg gccccccagt tgctggtggc aacagataag cccacgacaa
     6481 aaggcgagcc gctgatcagc gatattgttt ccgacgcgca acaagcggat ttactgatcc
     6541 ccgtggatga aacactgcct gtcatcaacg acgaacaatc cacatcaaca ccgttaacca
     6601 ccgctcagac gatgacgttg gctgcggtgg ctgacaaaaa tacgacaaaa gacgaaaaag
     6661 cggatgatct taatgaagac gtcaccgcaa gcctgagcgc cctttttgcg atgttgccgg
     6721 gttttgacaa tacgcccaaa gtgactgatg caccgtcaac cgtgttaccg gcagagaaac
     6781 caacgctttt cacaaaactg acttctgcgc aactcacaac agcacagcct gatgacgccc
     6841 ccggcacacc agctcagcca ttaacaccgc tggtagcaga agcccagagt aaagcggaag
     6901 tcatcagcac accttcgccg gtgactgctg ccgccagccc gctaatcact ccacaccaga
     6961 cacagccact gcccaccgtc gccgcgcctg ttttgagtgc accgctgggt tctcacgaat
     7021 ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa caaagtgcag
     7081 agttgcgtct gcacccacag gatttaggtg aagtgcaaat ttccctcaaa gtggatgata
     7141 accaggcgca gatccagatg atttcaccgc atcagcacgt acgcgcagcc ctggaagcag
     7201 cgctgccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg caaagcaaca
     7261 ttagtggcga aagctttagt ggtcagcagc aggccgcttc ccagcaacag caaagccaac
     7321 gcacagcaaa ccatgaacct ctgacggggg aagacgacga tacgcttccg gttcccgtct
     7381 ctttacaagg gcgtgtaaca ggcaacagcg gcgttgatat tttcgcctaa cgtcagaggt
     7441 agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg ataattagcc
     7501 gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga taagcaagaa
     7561 aagcaagcga tcgctttgga tcccgattct ggtattcatt accctcgcgg cctgtgccag
     7621 cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag cgcagcaacg
     7681 cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatt tgggcgatgc
     7741 ggatcgcgtg ctttatatcg gcataaccct gcgactgaaa gatgaagcaa cccgctcgcg
     7801 gctgagtgag tatttgccgg aagtccgtag tcgcttgctg ttactgtttt cacgtcagga
     7861 tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga ttaaaaccac
     7921 actttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg tgctgtatac
     7981 cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct gaaattgatg
     8041 cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt agcggcgaaa
     8101 gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa cgtttgcagg
     8161 cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg ttcaacctgc
     8221 tgcgccgtag cccggacatt accgtcgggg ccatccgcat tcagccgtac catgaatttg
     8281 cccgcaactt gccggtgccg accaacctga accttatcca ccttaaaccg ctgcgcggca
     8341 cagggctggt ggtgttctcg ccgagcctgg tgtttatcgc cgtggataac ctgtttggcg
     8401 gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tactcatacc gaacagcgcg
     8461 tcatcaaccg catgttaaag ctggcgcttg aaggctatag cgacgcctgg aaagcgatta
     8521 atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc aatatcacca
     8581 cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc aacctgaccg
     8641 gcgaatttaa tatctgcctg ccattcagca tgatcgagcc gctacgggaa ttgttggtta
     8701 acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac ctggtgcgcc
     8761 aggtgcagca ttcacagctg gaactggtgg ccaactttgc cgatatctcg ctacgcctgt
     8821 cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc gatcgcatca
     8881 tcgcccatgt tgacggcgtc ccggttctga ccagtcagta cggcactctc aacggtcagt
     8941 atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac gaggaacagc
     9001 ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg acgatatgtg
     9061 ggctgaagcg ttgagcgagc aaaaatcaac cagcggaaaa agcgctgccg acgcaatgtt
     9121 ccagcaattt ggcggcggag atgtcagcgg aacgttgcag gatatcgacc tgattatgga
     9181 tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca aagagctgtt
     9241 gcgtctgacg caaggatcag tcgtggcgct ggacggtctg gcgggcgaac cgctggatat
     9301 tctgatcaac ggttatttaa tcgcccaggg cgaagtggtg gttgttgccg ataaatatgg
     9361 cgtgcggatc accgatatca ttaccccgtc cgagcggatg cgccgcctga gccgttagtg
     9421 atgaataacc atgctactgt gcaatcttcc gcgccggttt ctgctgcgcc actgctgcag
     9481 gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgccgcctg gctggtaaaa
     9541 cggctgggat ttgcccctaa acgcactggc gttaacggtc tgaaaattag cgccagtgct
     9601 tcgctgggcg cgcgtgaacg ggtcgtggtg gtcgatgtgg aagatgcacg gctggtgctc
     9661 ggcgttaccg cagggcaaat caatctgcta cataaacttc ccccttcagc accaacggag
     9721 gagataccgc agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg tggcgggaga
     9781 tcctgatgcg tcgtttgttt tctgtcgcac cggtccttct ctggctgatt acgcccctcg
     9841 ccttcgcgca actgccgggt atcaccagcc agccgctacc tggcggtgga caaagctggt
     9901 cgctcccggt gcagacgctg gtgttcatca cctcgttgac gttcattcca gcgattttac
     9961 tgatgatgac cagtttcacc cgcatcatta ttgtttttgg tttattgcgt aacgcgctgg
    10021 gaacgccctc cgcgccacct aaccaggtat tgctgggact ggcactgttt ttgacctttt
    10081 ttattatgtc gccagtgatc gacaaaattt atgtcgatgc gtaccagcca ttcagcgaag
    10141 agaaaatctc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt gagtttatgc
    10201 tgcgtcagac ccgcgaggcg gatttagggt tgtttgccag actggcgaat accggcccgt
    10261 tgcaggggcc tgaagctgtg ccgatgcgca ttttgctccc ggcctacgtg accagcgagt
    10321 tgaaaaccgc atttcagata ggcttcacga ttttcatccc ttttttgatt atcgacctgg
    10381 tgattgccag cgtgttgatg gcattgggga tgatgatggt tcccccagcc accattgctc
    10441 tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg gtcggttcgc
    10501 tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc atgatgatgg
    10561 ggactgaagc gatgaaagtc gcgctggcac tggctgcccc actgttgctg gtggcgctgg
    10621 tcacgggtct tatcatcagt attttgcagg ccgccacgca gatcaacgaa atgacgctgt
    10681 cgtttattcc aaaaatcatc gccgtattta tcgccattat tattgccgga ccgtggatgc
    10741 tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat atcatcgggt
    10801 aggccgtaca atgatgcagg taacaagcga tcaatggctt tcctggttaa gcctgtactt
    10861 ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgcg ccaattctga gcgaacgcag
    10921 cgtgccgaaa cgggtaaaac tgggtctggc aatgatgatc acgttcgcca ttgccccatc
    10981 attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg ccgtgcagca
    11041 gatcctgatc ggcattgcgc ttggttttac catgcaattt gcctttgccg ctgtgcgaac
    11101 cgctggcgaa attatcggtc tgcaaatggg gctgtcattt gcgacatttg tcgatcctgg
    11161 cagccatctc aatatgcccg ttttagcgcg catcatggat atgctggcat tactgctgtt
    11221 cctgacattt aacggtcatt tatggttgat ttcactgctg gtcgatacct ttcacaccct
    11281 gccgattggt agcgaaccgt tgaacagcaa tgcgtttctg gcactcacca aagcagggag
    11341 tttgattttc cttaacgggc tgatgctggc gttaccgctc attactctgt tgctgaccct
    11401 gaatctggca ttaggtttac tgaatcgtat ggccccgcaa ttatccattt ttgttattgg
    11461 atttccatta actctgactg tcggcatctc tttaatggcg gcattaatgc cgttaattgc
    11521 gcctttttgc gaacatttat tcagtgaaat ttttaatttg ctggctgata ttattagtga
    11581 attaccatta atataattcc gtaacgttta tcatgctacc ctaaggatta tccgaaaaat
    11641 aatatctgcg aacatcttcc aggatactcc tgtagcgaaa tatttgtttt aagctcactc
    11701 acatatcgca acatttactt tactttaaga caattccagg caaattatac aacactttac
    11761 gggatagtaa gtccgcctga aaaattgcga gagtggcgca ttaggtgacc catgttgttc
    11821 cgtttagtca tgatgaaata ttcaggtaag gggaattatc gttacgcatt gagtgagggt
    11881 atgccatgtc aacgattatt atggatttat gtagttacac ccgactaggt ttaaccgggt
    11941 atctgttgag tagaggggtt aaaaaaagag aaatcaacga cattgaaacc gttgatgacc
    12001 ttgccatagc ttgtgattca cagcgccctt cagtggtgtt tattaatgag gactgtttca
    12061 tccacgatgc ttctaacagt cagcgcatca agcacatcat taatcaacat cccaatacgt
    12121 tatttatcgt ttttatggca attgccaatg ttcattttga tgaatatcta ttggtcagaa
    12181 aaaatttatt gatcagctct aaatcgatta aaccggaatc tctcgacgat atccttggcg
    12241 atattctgaa aaaagataca acgataacct cgtttttaaa tatgccgact ttatcattga
    12301 gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg tcagggaacc attcaaatct
    12361 ccgaccaaat gaatatcaaa gccaagaccg tttcatcgca taaaggtaat attaaacgta
    12421 agatcaaaac gcataataaa caggttatct accatgtcgt ccgactgacg gataatgtga
    12481 ctaatggtat ttttgtcaac atgcgctaac acattctgac tggtggtttc ccaccagtca
    12541 ggctgaataa gattactctg ctttctcaac aaagataccg tcctgatgcc ctgcttcatt
    12601 aaagaaccag atgccgagcg ggtagtcttc cagcgaaacc aggtacattg tgccttcact
    12661 aaactcctca actgccagta ccacgccagg acgacgcgga ccgccatccg ttttgactgt
    12721 tacccgatca ttcaccttca tcgttttcct cctgtggctt tgtgccagtg tagaacaatt
    12781 tcgtttttct ggcagcgcca ggcgcgcgcg agtgctgatt ttctcgacgg tctatactta
    12841 agagatgcca ggcggactta acgactggcg gcaacaacag agtaacggtt gcgaggaaag
    12901 atgatgaaaa ccgcaaaaga gtacagcgat accgcaaaac gtgaggtcag cgtcgatgtc
    12961 gatgccctgc tggcggcgat caatgaaatt agcgaaagcg aagttcatcg cagccagaac
    13021 gattctgaac acgttagcgt cgatggacgt gaatatcata catggcgtga attggcggat
    13081 gccttcgaac tggatattca tgacttcagc gtctctgaag tgaatcgttg aatgcacaat
    13141 aaaaaaatcc cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga agcacttaaa
    13201 aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atctgcaatt tttttcgctg
    13261 acaagaaaat attcgcgcta tgaatgatta acgctactta tgaatagaaa tgtgacgcgc
    13321 gtcacccctt ggtggctctg gtgtcaacgg gatcgctccc gaaaaaatca tctttgatta
    13381 tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcccatgt tttcaattca
    13441 acaaccacta ctggttttta gcgatcttga tagcaccctg ctggacagtc atagttatga
    13501 ctggcaaccg gcagccccct ggctcagccg tttacacgaa gccaatattc ccgtcattct
    13561 ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa aaaatgttgg ggctacaagg
    13621 cttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat ggcaggatat
    13681 agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatctgcc aggttttaaa
    13741 tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg acgatgcaac
    13801 cattgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc
    13861 gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta tcgctcgtct
    13921 gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac tggatgcctc
    13981 tgccggaaaa gatcaggctg ccaactggat taccgcgacc tatcaacaat tgtcaggcag
    14041 acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcctttac tggaggtaat
    14101 ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtccatctgc atgatgagga
    14161 tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg aagggctgga
    14221 ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattac gcccggcctg
    14281 ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat cataatcacc
    14341 ggtttcctcg ctgctactta cccccagcga ggcactgatg cgtatcgttg tactcttggc
    14401 gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct ccgcagcctg
    14461 cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc gcccggcaac
    14521 gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa cacggtcgcc
    14581 cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga cctgaatgac
    14641 agaaaaagga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt tttcgaacaa
    14701 tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact gcaacgagct
    14761 ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga tgagtaacat
    14821 ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga aatcgccgcg
    14881 cacgccttcg cttagcgtat ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc
    14941 ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt ccattgcctg
    15001 cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt caggatttga
    15061 agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga ggtttttatc
    15121 gatggcgttt tttaaaaatt gctgcatagt acgcacggga atactcatcc ccagcacgcc
    15181 ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt cagtattgct
    15241 ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt ctcgttgtga
    15301 atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta cattgcgcgt
    15361 aaacaaggtc ggcagcgtcg aaacgtaaaa tccagcacgc gagacataca ccgcctgttc
    15421 aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt
    15481 aatttcattg tcgaggcttt cattttcgcg agacaggaga ttcccctcgc tgactaatgc
    15541 atccgatacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt gccaggcgtg
    15601 ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga aatccagtgg
    15661 cgccacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga cgttatactg
    15721 caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctggc tggagatata
    15781 ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa gcaataccac
    15841 gataaagcag agattaacga catgaccggg ccccaggcgg cgtgcgagtt ttttcaacca
    15901 gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc tgctttttta
    15961 tgattctggc ataacccggc gatagcgtac cgcaaaacag cgataatttt ataatggtgg
    16021 catgataaga cgcgactatc attcattgca ggcattggga caccaggccg gatgtgatcc
    16081 ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga
    16141 acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt acccgtcata ccaacgacac
    16201 tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc tttaaccgta
    16261 acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac
    16321 cttagttgaa gatggcgaat tttgcataat agcctgtgcg agtattaatg tgcctgaccg
    16381 cgcattttcg ctacggcttt cactcccagc accacgatgc cgccgatgat aaatccaaga
    16441 atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgaaa
    16501 tgttcaatgg cgtgatgcaa cggcgcaata ccatgtacca caatcccgcc gccgacgagg
    16561 aacatcgcca gcgtgccgac aatcgataac gctttcatca accagggcgc aatgatcaat
    16621 aaccctttac ctaatgcctg catcagcgcg ctggattttt ccgccagcca ataccccagg
    16681 tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg
    16741 atgcctgaaa gcaccagcac ctgattaagc aacggcgctt ctgccacaat ccccagcgtg
    16801 atggcgacga tttccgcaga caatataaaa tcggtacgaa tcgccccttt tattttgtcc
    16861 ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg actctgcgcc
    16921 ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac cccttcaaag
    16981 cagagaaacg cgccgccaat catcaacagt ggcgtaatcg cccacgggat aaacgcactg
    17041 atgatcagcg ccagcggcac cagaatcact ttattgatca gcgaaccttt cgccacgccc
    17101 cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc attgagcgat
    17161 aagtcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacggagata
    17221 tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc
    17281 attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat
    17341 acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct ctcgccagca
    17401 gaatgatttt cacgtttact atgaaccacc tttttatttc agccgtgagg gattatgcgt
    17461 ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca
    17521 acctattttg tcattcggat tggcgtggaa agctggcctc cgttaatgat ggcgggcgtt
    17581 cgattcctgg cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactt
    17641 cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct ggctgtcggt
    17701 aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgtagtg
    17761 gttgcaaccg tgcccctttt taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc
    17821 aaactggaat gggtgggtat cgccattggg cttgccggaa tcatcatgct caatagcggt
    17881 ggaaacttaa gcggcaatcc atggggcgcg attctgattt taatcggctc gattagctgg
    17941 gcgtttggct cggtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg
    18001 attgagatgc tggcggctgg cgtggtgtta atgattgcgt cgatgattgc gggtgaaaaa
    18061 ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg gctatctggc gctgtttggt
    18121 tcgattatcg ccattaacgc ttatatgtat ttaatccgca acgtcagtcc ggctctcgcc
    18181 accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg actgggtggc
    18241 gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgtcttcgc ggtggtactg
    18301 gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt tattcaggat
    18361 gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca
    18421 gatccactct tccagacgct cggtaagcgc cgcatccgta agcttctcgc gcccacgtaa
    18481 cgcacactcc cagacaatca gtacgcgcca gccgagttcc tgcaagcgac tgatatcgcg
    18541 gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcggttc gagtcgcagg
    18601 cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa atatcacgca
    18661 gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa
    18721 cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt
    18781 ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg
    18841 cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg
    18901 cggcaaagac cggcacgacc accgagttac cgaactggcg ataggcctga gtgtccgaaa
    18961 ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc gcgcattcac
    19021 gaggtgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaag tctttctcac
    19081 ccgtggccat atcccagccg cggtcgatta aaatttccgc gccatccttg taataacgag
    19141 cagacagcgt acgggtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc
    19201 cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac agcaccggcg
    19261 tcaggatata tttcgcctca accatcgggt ccagcagctg cgccagcgtc actcgctgtg
    19321 cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagattc agatcgcgac
    19381 gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgttta ccgtcgatga
    19441 ttttcggatc gtccggcccg ttatctgctg cgtcggcaac gtcatagccc agttcgtcca
    19501 gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg actcttcagg tttttgacat
    19561 tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca
    19621 gcgtaccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata
    19681 cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg tgttcaggaa
    19741 tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga tgactgaggg
    19801 tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa tggttggctt
    19861 tataagtgcg tacagcatgt ttgttccatt cgctggtaaa cacgcactgt ccgccaatcg
    19921 attcaaaacc gcgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat
    19981 aatgcggatg atgttccggt ggtttgggta acaacgtttg cagatgggca aactctttct
    20041 cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc
    20101 agtgattctc acccacacca ttaagctgcg ccaccagcgt tttaacatca taaatttgca
    20161 gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg gttactgata
    20221 tattttcctg catagattta accggccatc tgagatggcc g
//
LOCUS       DAJOTB010000085        19959 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJOTB010000085 DAJOTB010000000
VERSION     DAJOTB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.085"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1314
                     /gene="argA"
                     /locus_tag="OHN28_003003"
     CDS             <1..1314
                     /gene="argA"
                     /locus_tag="OHN28_003003"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCQ2298355.1"
                     /translation="TELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIG
                     LLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDI
                     TARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHRQLD
                     SGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSE
                     LFPNEAQTRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFS
                     RDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTII
                     QRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLF
                     VLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1396..3222)
                     /gene="recD"
                     /locus_tag="OHN28_003004"
     CDS             complement(1396..3222)
                     /gene="recD"
                     /locus_tag="OHN28_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCQ2298356.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEVLAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(3222..6764)
                     /gene="recB"
                     /locus_tag="OHN28_003005"
     CDS             complement(3222..6764)
                     /gene="recB"
                     /locus_tag="OHN28_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCQ2298357.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(6757..9645)
                     /gene="ptrA"
                     /locus_tag="OHN28_003006"
     CDS             complement(6757..9645)
                     /gene="ptrA"
                     /locus_tag="OHN28_003006"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCQ2298358.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9821..13189)
                     /gene="recC"
                     /locus_tag="OHN28_003007"
     CDS             complement(9821..13189)
                     /gene="recC"
                     /locus_tag="OHN28_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCQ2298359.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13202..13525)
                     /locus_tag="OHN28_003008"
     CDS             complement(13202..13525)
                     /locus_tag="OHN28_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCQ2298360.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(13510..13917)
                     /locus_tag="OHN28_003009"
     CDS             complement(13510..13917)
                     /locus_tag="OHN28_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCQ2298361.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(13914..14477)
                     /locus_tag="OHN28_003010"
     CDS             complement(13914..14477)
                     /locus_tag="OHN28_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2298362.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(14468..14938)
                     /locus_tag="OHN28_003011"
     CDS             complement(14468..14938)
                     /locus_tag="OHN28_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2298363.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(15123..15917)
                     /gene="thyA"
                     /locus_tag="OHN28_003012"
     CDS             complement(15123..15917)
                     /gene="thyA"
                     /locus_tag="OHN28_003012"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCQ2298364.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(15924..16799)
                     /gene="lgt"
                     /locus_tag="OHN28_003013"
     CDS             complement(15924..16799)
                     /gene="lgt"
                     /locus_tag="OHN28_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCQ2298365.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(16950..19196)
                     /gene="ptsP"
                     /locus_tag="OHN28_003014"
     CDS             complement(16950..19196)
                     /gene="ptsP"
                     /locus_tag="OHN28_003014"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2298366.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19209..19739)
                     /gene="rppH"
                     /locus_tag="OHN28_003015"
     CDS             complement(19209..19739)
                     /gene="rppH"
                     /locus_tag="OHN28_003015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCQ2298367.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         4780 a         5570 c         4983 g         4626 t
ORIGIN      
        1 accgagctgg tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa
       61 acgtttgtca tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt
      121 aatgatatcg ggttgttgca cagcctcggc atccgtctgg tggtggtcta tggcgcacgt
      181 ccgcagatcg acgcaaatct ggctgcacat caccacgaac cgctgtatca caagaatata
      241 cgtgtgaccg acgccaaaac actggaactg gtgaagcagg ctgcgggaac attgcaactg
      301 gatattactg ctcgcctgtc gatgagtctc aataacacac cgctgcaggg cgcgcatatc
      361 aacgtcgtca gtggtaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat
      421 tactgtcata gcgggcgtat ccggcggatt gatgaagacg cgatccatcg tcaactggac
      481 agcggtgcaa tagtgctaat ggggccggtc gctgtttcgg tcactggcga gagctttaat
      541 ctgacctcgg aagagattgc cactcaactg gccatcaaac tgaaagctga aaaaatgatt
      601 ggtttttgct cttcccaggg cgtcactaat gacgacggtg atattgtctc cgaacttttc
      661 cctaacgaag cgcaaacgcg ggtagaagcc caggaagaga aaggcgatta caactccggt
      721 acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat
      781 ttaatcagtt atcaggaaga tggcgcgcta ttgcaagagt tgttctcacg cgacggtatc
      841 ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatt
      901 ggcggtattc tggagttgat tcgcccgctg gagcaacaag gtattctggt acgccgttct
      961 cgcgagcagc tggagatgga aatcgacaaa ttcaccatta ttcagcgcga taacacgact
     1021 attgcctgcg ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg
     1081 gcagttcacc cggattaccg cagttcatca cggggcgagg ttctgctgga acgcattgcc
     1141 gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac
     1201 tggttccagg aacgtggatt taccccagtg gatattgatt tactacccga gagcaaaaag
     1261 cagttgtaca actaccagcg taaatccaaa gtgttgatgg cggatttggg ttaagcgatg
     1321 caaatcgccg gatgcgacat gcacaacatt cataggtatg ataagaccca catgcgtcgc
     1381 atcaggcaat tacgtttatc cccgtgaact aaacaacgcc gccagaccac tgcgccgctc
     1441 ggtgcgcgtg gcaattgccg cacttaaaat gcgctcatcg gcatacagcg acagacggcg
     1501 acgcgcgcgg gtcaccgcgg tgtaaaccag ctctcgcgtt actaccggcg tgcgttgact
     1561 cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc
     1621 ccacgtcgtt tcgtgctctg gcaggcgact cggttgcaca gacttaatat tgccgtccgg
     1681 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccaa tatcgccatt
     1741 aaacaaccca agcgcgctgt cattacgggc aatcatcact ggtcgacctt cgtaccaacg
     1801 agagtgcgga tgacgatgaa ttttgcgctt ctgttgcata aattgctcaa ttcgctcatt
     1861 caatccagcc acgccaaacg gtccttcccg cagggcgcac aaaagctggt actcattgaa
     1921 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccgta
     1981 acccgcaaga acttcctcaa gcatcgcaat ataatcttcg ccactctgta aaagccgttt
     2041 ttcgatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat cacctcggtt
     2101 aatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
     2161 caggcagagg ctgtcgcgca aagatgccgc ttctgtgcca gttcctgctg gaacgtggct
     2221 tcccgtcagg cggctcaact gcccggcacg ctcggcggta aagcccgcgt tggcataagc
     2281 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
     2341 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggaagatc
     2401 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
     2461 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
     2521 cggaatgcgt ttcttttgtt cagcggtcag cggtaactgt cgcaaagcct tgccgagaga
     2581 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
     2641 tccgtcggcc atttgaatta aggctgccag caacttcgct acggtggtcg ttttaccggt
     2701 gccaggaccg ccggaaatca ccgaaatccg ccgcgtcagc gccactgccg ccgcaacttt
     2761 ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg ccagtagagc
     2821 ttcatcaacc tcaatggcat gattcacttc gttgaaaaaa cgtgccactg tgcgctcgtt
     2881 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg ttggttcatc
     2941 tccccggctg accgcttgtg aagccagcaa gcattcttcc caattttgta gctcaccgat
     3001 ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca gtcgtgaaag
     3061 cggcaaacaa acgtgtccct ctccggcatc atgacttaat agtgccgccg cgagggtgac
     3121 ggcaggatgt tcatctcccg ccacggtcag ggcaaactgc acgtccagcg ggcgtagctg
     3181 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
     3241 ataccggcaa acatctcatc catcaggtca atcaacccgg cgttgggtcg ggtcgcgtag
     3301 atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagataaat aacgccgcca
     3361 aagtggcgct catagtcgta atcagcaata cgatggcgca gataacggtg cagcgccagg
     3421 gtataaagct gatattgcag atcatagcgg tgtgcctgca ttgccgctgc catagcctgt
     3481 tgggtgtaag ccgaactgtc ttcacccaac cagttggatt tatagtcgag caggtaataa
     3541 cgcccttcgt ggcggaacac cagatcgata aagcctttta acatgccacg tacctgcatg
     3601 aactccagcg gcgggcagcc agcagatagc gggtcaaact ggcgaattag tgcatcaagc
     3661 tggctggcga tgagcggttc gctaattggc aggtaaaact ccatctccac ctgtttatcg
     3721 cgatcggaaa gctgactcag gctaataccc gtttcattga gaggtgccag gaggacagcc
     3781 gtgatccact cagtcaacac cggttcccac tgcggttcaa agccaccgag ctccagtttt
     3841 tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc
     3901 aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt cggtacttca
     3961 acgacgctga ccacgcctgc ggcatcaaca tccaaccgtg gcatcagatc ctgagcgata
     4021 ccgtgaccac gctgctgcaa accggagtag ctggtgacac gccagttatc gccgggcaat
     4081 cgttgtagcg tcctcgcatt cagttctgca gttaatgcat cattaacctg ccagggctgg
     4141 ttatcaccaa tttgtgccgt ttgccaggca atatcatcat cgcacaacgc ttcaatgcag
     4201 gtacgaagcc ctgccacatc ttgcggttct cctttttgca gcaaacgccc gagcgcactt
     4261 tggtggacgt cggtatcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
     4321 agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gtaaacgcag atcttccgcc
     4381 agacgttcgg cctccgcgag gtcgacgctt tctggcgcag cattaagatc cagaacggcc
     4441 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
     4501 ggcaaccaga ccagcggata ttccagccct tttgatttgt gtatcgtgac aatctgcacc
     4561 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
     4621 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
     4681 tgtagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
     4741 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
     4801 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc ccacgcatgt
     4861 tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga cgttgccagc
     4921 gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacag ccaaagcatc
     4981 tcctgtgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac ggaagggatt
     5041 tccagcagcg ttaaggcatc gcgcacctga gcggcctcct ggcggctgcg caccagcaca
     5101 ctgatgtccg aagcacgcac cggacgcgca tcgtcgccgt tcatcagcaa cgcttcgccc
     5161 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
     5221 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
     5281 atttcacctt taaacacaaa gcgtaacgcc tgatttttcc cggcagattt cacaggaata
     5341 aacggtattt cacgaaacat gaacgcgtca tcagtctggc tgaaaagttt gttaacgctg
     5401 ttcaccattc ccggtgcgga acgccagttg gtatctaagg tgtagtgagc atgaacttcg
     5461 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgtttc
     5521 gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt gccagatacg gcgaaaaatt
     5581 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
     5641 cggatcgccg ctgccaatac ctcgccgctt tcactacgca gcgcggaatc gagccgactt
     5701 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
     5761 atctcagcca atgcgcgggt gatcaccaga tcgcggatcg acaatggttc tgcaagcagt
     5821 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct
     5881 tctaagaaac gctgagagaa tttttccagc gactccggca actgatagct attggtctct
     5941 tcttctgccc aggcgctgat cttctcgatc catttagcct gattgctacg gttaaacttg
     6001 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
     6061 tgctgtttta cggcatcaat acgcgccaca atttgcgcgt ggcgggaagc cagcgtttca
     6121 tcatcggggg gcggtgcttt gataactggc gcttcgcctt gcagataacg attaatatcg
     6181 cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggcaat ttcacgcggc
     6241 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
     6301 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
     6361 cgctggcaaa agccgtggat agtaaagact gccgcttcat ccatctgccg ctcggctaac
     6421 aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggcg tttgtacagt
     6481 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
     6541 cgaccacgca attctgccgt ggcagcctcg gtaaaggtca ccaccagcag ttcttcaacg
     6601 gtcagcgggc ggggaaaggc ggccgaaccg cctagtccaa gtaacaggcg caaatagagc
     6661 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
     6721 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
     6781 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
     6841 atattcggct ttcccattct ggctgccgga aatctgcgac agaatagcca taccttgcgg
     6901 ctcgaccacc gcctgatgga agaaatcagc aattttttgc ggcgtcagca gttttatctg
     6961 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
     7021 cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg
     7081 ctggatttgc gcaaactctt ctggcttcat cgcccgcaat tttgcctctg cggttgggaa
     7141 aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt gcaaaaggaa
     7201 gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat agcccaactg
     7261 ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca acagagaact
     7321 atacgctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg ccagtgcgga
     7381 gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga ccacgacatc
     7441 tttgttacga caccactctg aaccatcagc gcccaactgt ttttgcacat ggcgtgccag
     7501 cgttattgcc tgggcttcag tcatgttgcc gataaccata aactctggtc tagcccctga
     7561 ttttaaggcg tcgcgatagg ccagcacctc tttcaacgta atggagggca aaattttgcg
     7621 ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca taatcgcctg
     7681 ctcgaacgct ttgccctttt ctgcggaatc catcatctgg ttataccagg acttcgcctg
     7741 ctcaagctga tcttccgtag cggtatagct aaagtagccc tccagcagag cctggaacag
     7801 ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt tagcattggt
     7861 ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc
     7921 gagataatca ttgagtgcaa acatcacctg attgcgggcg ctgtccatgg ctttcggatt
     7981 acgcaaaatc aggctgacat cagctttggg ctcgctggca aaataacggc ttggcgcata
     8041 caccacgcgc agattcgact catcaacaat caactctgga tggtcgtatt tcttctctga
     8101 cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg agagcgcaat
     8161 attggcggct tttttctgcc agtcagcgaa agtttgtgcg ctaattttat cgacctgata
     8221 cggcgcatcg acaaagtaag ccgttttgtt gtgcggctct ttcgggctga tataccagat
     8281 acgcgcattc tgtggcgtca tcatcgccag acgttctttt accgctttag cgtcgtaccg
     8341 atcggcaata ttgactgcat ccagtgtatg ctcaacagga acgcgaatca tggtatctgc
     8401 cagccattcg acgtaatcca tatcacgggt gattgacgga taacggaagt cgatatccag
     8461 cacattcgcc agttcatcga agtattgttt atcgatccct ttttcacgta acagattgag
     8521 gtagctaaaa atggccgcca caacctgatc gcgattcgcc agacctttat cggttaaaga
     8581 cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggaat tggcgctaat
     8641 gccctcaact aatccctgct tttgcagcca gtcagaaagt gtaccagggc tgcgattacc
     8701 aatcagatag gtaatcaatt catccgtttt actacggaac ttcgctgaat tgttatcgat
     8761 gcgaaactca acgcgcagaa ctttacgcgg cagcgccggg acgtaatgaa tgataatgcc
     8821 cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct ctttgttcgg
     8881 cacgcgacca aaggtgtccg ccgccatttt cgccaactcc ggcaacggtt tattgctgta
     8941 aataaccgcc ttcatcagat tggcggaata gtacttctcg tggaaatctt tcagcgcctg
     9001 ctgtaccgga ttacctggtt tgtcgcttaa agtttcgagg ttaccaccag aaaactttga
     9061 accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca tcccgtcacg
     9121 cgtacgcgcc atggttaatt cggcattcac tgcattacgc tcacgttcgg cgtatttctt
     9181 gtcgagcaag ggttctgcaa tagcatcggc caggcggtct accgcaccgg gtaaggcatc
     9241 gttctcaact tccagataga aagccgtgcg atacggtgcc gtgctggcat tgtgactgcc
     9301 gccgtgcatt ttgagatatt cggccagact gtcagcctgc ggatactttt tcgaccccat
     9361 cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcaggat cttccagcga
     9421 cccaacgggc accaccagcg ccgagagcga tttaactgcc tgcggatcag aaaccagtaa
     9481 gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt tatcactttt
     9541 acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg gtgcccagag
     9601 ggcaacaaac aacaataatg ctttgaacca gatgctgcgg ggcattcacg gacctcataa
     9661 tcaactttat tttctggcca gattcaacac gttaacgcaa atcatctgcc agaatttaat
     9721 cttgtgctga acgagtcagc ctatgtctat ataaccatca gtccgtgact ggtgcgcatc
     9781 ataaagtaag cggatagatt gcgcaatttt tatacaacac tcatgactga ttaaagcgaa
     9841 acagcggtaa caggaaacgt tgcgactgtt caacgatagc ctccattgtc tctggtgtta
     9901 attgccgcca gagccgttga taccagatat catcaccttc gccacgcacc atcatgttgc
     9961 cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca
    10021 tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt tcaggtaaca
    10081 ccagcaatgg cgcggacatt ccttcacgat acccctcaat cagttgtgag aggtaatgca
    10141 aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc
    10201 gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa agttgcattc
    10261 cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc tgcacctgcg
    10321 gcaaccagcc cgttatttgc acaccgttgc aggcgagatc aatctccata ctctgccccg
    10381 gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctgacac tgcgtttccc
    10441 agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag cgacggaaca
    10501 agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg atttgataac
    10561 ggctaagtcc ttccagaata aatggctcgg tgtcggggat ttcgctgtct tcggtacgga
    10621 agttcacctg caaacgcatc tggaaaaatg cccgcaccgg atgtgcccag aatcgttgta
    10681 gcgtttccag cggcacggtt tccggtaagg taaatggcag cggctgaaca aattcagaat
    10741 gtgctttacc agcctggctg gccgcaggta gccattcacg agcataactt tgtcgttcgc
    10801 ctggctggta gttttgtgga tcaaacggca ttcgggtatg gaggcaagta agatgcgctt
    10861 ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga taatggcttt
    10921 gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca ctgttatcct
    10981 gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt aacgcttcca
    11041 ggaacagata gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt ttctggctca
    11101 tcagatcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc attcccagca
    11161 ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg ttaaccggtc
    11221 cggcgagaaa acgctggctg atacgttctt gatccaggcg ctgtgccagt tcatcacgca
    11281 atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg ataatcgcct
    11341 gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc aggaagaagg
    11401 cgttgagcat atcgcgacaa accggcaacc actcttccag cggacgctcc tgcgccagcc
    11461 cgcgacgcca gatatttagc tgcattagca gtgaagccag atgccccacc agttctgcaa
    11521 ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc gcgctctcca
    11581 tcgcgtagcc cagcaacatg cgcgtcaggc caaaccgcca ggtgtgttga ccggtagcgg
    11641 gaagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg gactcgttga
    11701 cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg
    11761 gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc aacgataaca
    11821 ggctgataaa cgcttcaagt acaggatgcg actgccgcgc ccgacggtcg gaaatggcgt
    11881 aaggcaggta acgatccgca ggtgcgctac caaacacagc ctgaataaac ggactgtagc
    11941 tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg tcttcctcca
    12001 gcatcgccag caggcgatcg tgtaaaactt caacttcacg ctgcgggcta tggcaaacgt
    12061 ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta cgggaaaact
    12121 cttcgatgtt cacaccagca acggcgcggt tttccagttc cagaatgtca gactgaatat
    12181 tatgcagcag gttatctggc gtcacatcga caaaagcgtc cagctcctgg ctgctctcca
    12241 ggtcagaaag gagataaatg tagtcgcgcc caagcttgcc ccatgaagcc agcagcgggt
    12301 tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg ctgtcgcgaa
    12361 atagcggtaa ttcgcgatct tcaaaactgt gtcggcgctg gcgagtcagt agtttcgcca
    12421 gataagcggg atctttaatg tcgccccagt aataacggca ggggttggta aacaggagat
    12481 ggatttcaat atgtttaccc agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg
    12541 aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc gcggactcca
    12601 gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgc ccaagttcgt
    12661 cggtatattc caccaacgcc ttccacaacg gcgcttgcca ggcctgtgct tctcccaacc
    12721 cttctaccag atgtcctgtt tcccactgtg ccagccagtc cggacgatag accagatact
    12781 ggtcaaacag gtccgccgct tttgaagaaa gctggaacag ttttcgcttg tcactatcgt
    12841 cagtcagata atgccgcaac agggtaaagt cttcgcgctc caacagttgc ggcagcagtg
    12901 tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg atctccggta
    12961 acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca atgtttgccg
    13021 caataccaaa cttttgcgac agggtcattt gcagccactg tgccataccg gtactttgca
    13081 ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca ataaactcca
    13141 tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata gcggctcctg
    13201 actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa
    13261 ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc
    13321 agttagcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccagccatgc cgccagagct
    13381 gttggtactg gtttctgctg gcaagactgt tcattaatgt tcgctggaaa cccgataacg
    13441 cagtgacaat catcaccatc aacaccatcg ccaacattac ctccggcaga ctaaagcctt
    13501 gttgattcct cagggaagct gacataacgc cctctctttc agcggacaaa aatcgctcca
    13561 gccgcgtggc gaaaaaacaa tgtttccatc gatgacttcg cctgttcgcc acaacgaaac
    13621 gccttcataa ccagcaatca ataaggcttc attatctgcc agtaaacgca gacaaacccg
    13681 ggcaccggtg gcagcataca gtaagcactg aactgcgggc agcgtctgcc aggagtgcat
    13741 ttttccccac tccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttccat
    13801 gctcacgcga gaagcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc
    13861 caaaatcagc agcatcagga ccagagccag tgacgaaaca cccttttcgc ggttcacagg
    13921 ttggatcctg tcacgctata gctggcatcc accacggttt gcggatcagc cttgctggca
    13981 gcgtgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga
    14041 aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt accttcacag
    14101 gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtcg
    14161 gactctttta ccggttcgcg atcccagata ccattactgt tcgcatccca ctgcacaatg
    14221 acacagtcac cctgtccgac aatttccagc ccttcgcccg tacagctgcc atggcaataa
    14281 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
    14341 gccagcttac gggtgttcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
    14401 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
    14461 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
    14521 gcgaaaccac tagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
    14581 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
    14641 gccagcgcgg cacaaagacc aatggtgaac tgccatgaca ggtattcgcc ccagcaacgg
    14701 aactcacaag gcaccataac gtcccctccc tgataagact gatactgtgg tcgcggttat
    14761 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
    14821 aaagccgttg cgactgctgc cagtattgcc agccatagag cccacttgcg cttagcatga
    14881 ccagaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt tttttcatgc
    14941 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
    15001 ttccggacta tttacgtcgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
    15061 tttaacggag ggcaaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    15121 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtatccttc aatctcaaag
    15181 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    15241 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatggttgct gtacagatgc
    15301 gtgtcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata
    15361 tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag
    15421 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    15481 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcga
    15541 cgggaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    15601 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
    15661 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    15721 tcgccctgca ggaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttagtt
    15781 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    15841 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    15901 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    15961 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    16021 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    16081 atgcgaaacg cgccgtaacc aatcaggaac aagcctgaga cagctcccat tgggcgcggt
    16141 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagctcgtaa
    16201 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    16261 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
    16321 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    16381 ccaagaccaa acggaatgag tggagcaata aaatcagaga cctggaagaa ggaacgttta
    16441 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
    16501 atgccgccgt cccagacacg gaaaagatac agcggatcgg ccataaactg cgggaaattg
    16561 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
    16621 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg tcgtgttgcc
    16681 agccacattg caaaaatgaa acccaccaga tacatcagtc cgtaccagtg aagcgccacg
    16741 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    16801 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    16861 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    16921 accgttaaaa gatatgtata tgatccgcgc tataaccccc cacgaatcag cccgcccatg
    16981 ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    17041 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
    17101 acgcgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    17161 gccacgcaca tcggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    17221 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    17281 atgttcgcca cccgggtatt gttgcgatca acggccagga tgtattgcgt cagatcgttg
    17341 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa caccattgac
    17401 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    17461 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggcttgtg
    17521 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    17581 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    17641 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    17701 gtacgcaagg tgacgggttt atcattaaac atttgcagca tcccctgata ctgcgccacc
    17761 tgttcttctt ccgacggaaa accactttgc agcatgaatg ggatttcagt gcgataaagt
    17821 cctatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accggcattg
    17881 agcatgactt taatgcgttc gccgcttttt aactgggcgg gtaaattgac gtcatcttcc
    17941 gccagacggc taagctcgat ctcttcacta attagccgct gatattcttg cagcagtacc
    18001 ggctccggat cgaccagcaa ttcaccgcga taaccatcga cgatcagcgt ccgacgatgc
    18061 agtaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    18121 atcgcagcat gggagttggc agcgccatcg cgcacgacaa caccgactaa gcgatcctgg
    18181 ggcagctcag caagcgttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    18241 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg accaagtgca
    18301 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    18361 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    18421 aacaattcgc gacgcagccg ggtatccgaa agcaggtgtg agtaaagatc gaaaatagcc
    18481 gccgtttctt tttgtgcacc ggcagcaaag cgtttgctat agcggcgaaa ctcgttagcc
    18541 gcctcttcca acgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    18601 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    18661 ccaggtgctg ccggtaatgc acggattcgc gtctggcgat attgcccaaa caaggcagtc
    18721 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    18781 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    18841 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    18901 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    18961 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    19021 ttcagtcccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    19081 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    19141 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    19201 ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt
    19261 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    19321 ttaaatgaca ccacctgtct gactggatac cagtaactta cccatcgcca gccgtcaaac
    19381 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
    19441 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    19501 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    19561 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    19621 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    19681 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    19741 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
    19801 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    19861 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    19921 gttgtgaaga actacggaat tactacggga aaacccgga
//
LOCUS       DAJOTB010000086        19660 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJOTB010000086 DAJOTB010000000
VERSION     DAJOTB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.086"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            29..583
                     /locus_tag="OHN28_003016"
     CDS             29..583
                     /locus_tag="OHN28_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2298368.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLSAH"
     gene            723..1058
                     /locus_tag="OHN28_003017"
                     /pseudo
     CDS             723..1058
                     /locus_tag="OHN28_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(1128..1610)
                     /locus_tag="OHN28_003018"
     CDS             complement(1128..1610)
                     /locus_tag="OHN28_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCQ2298369.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1845..4427
                     /gene="leuS"
                     /locus_tag="OHN28_003019"
     CDS             1845..4427
                     /gene="leuS"
                     /locus_tag="OHN28_003019"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCQ2298370.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4442..5023
                     /gene="lptE"
                     /locus_tag="OHN28_003020"
     CDS             4442..5023
                     /gene="lptE"
                     /locus_tag="OHN28_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCQ2298371.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5023..6054
                     /gene="holA"
                     /locus_tag="OHN28_003021"
     CDS             5023..6054
                     /gene="holA"
                     /locus_tag="OHN28_003021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCQ2298372.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6056..6697
                     /gene="nadD"
                     /locus_tag="OHN28_003022"
     CDS             6056..6697
                     /gene="nadD"
                     /locus_tag="OHN28_003022"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCQ2298373.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6721..7332
                     /locus_tag="OHN28_003023"
     CDS             6721..7332
                     /locus_tag="OHN28_003023"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCQ2298374.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7592..7909
                     /gene="rsfS"
                     /locus_tag="OHN28_003024"
                     /gene_synonym="rsfA"
     CDS             7592..7909
                     /gene="rsfS"
                     /locus_tag="OHN28_003024"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCQ2298375.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7913..8380
                     /gene="rlmH"
                     /locus_tag="OHN28_003025"
     CDS             7913..8380
                     /gene="rlmH"
                     /locus_tag="OHN28_003025"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCQ2298376.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8411..10312
                     /gene="mrdA"
                     /locus_tag="OHN28_003026"
     CDS             8411..10312
                     /gene="mrdA"
                     /locus_tag="OHN28_003026"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCQ2298377.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10315..11427
                     /gene="mrdB"
                     /locus_tag="OHN28_003027"
                     /gene_synonym="rodA"
     CDS             10315..11427
                     /gene="mrdB"
                     /locus_tag="OHN28_003027"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCQ2298378.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11438..12526
                     /gene="rlpA"
                     /locus_tag="OHN28_003028"
     CDS             11438..12526
                     /gene="rlpA"
                     /locus_tag="OHN28_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCQ2298379.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12666..13877
                     /gene="dacA"
                     /locus_tag="OHN28_003029"
     CDS             12666..13877
                     /gene="dacA"
                     /locus_tag="OHN28_003029"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCQ2298380.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13987..14250
                     /locus_tag="OHN28_003030"
     CDS             13987..14250
                     /locus_tag="OHN28_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCQ2298381.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14351..14992
                     /gene="lipB"
                     /locus_tag="OHN28_003031"
     CDS             14351..14992
                     /gene="lipB"
                     /locus_tag="OHN28_003031"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCQ2298382.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15252..15776
                     /locus_tag="OHN28_003032"
                     /pseudo
     CDS             15252..15776
                     /locus_tag="OHN28_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            16017..16982
                     /gene="lipA"
                     /locus_tag="OHN28_003033"
     CDS             16017..16982
                     /gene="lipA"
                     /locus_tag="OHN28_003033"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCQ2298383.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17082..17285)
                     /gene="tatE"
                     /locus_tag="OHN28_003034"
     CDS             complement(17082..17285)
                     /gene="tatE"
                     /locus_tag="OHN28_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCQ2298384.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17414..18202)
                     /locus_tag="OHN28_003035"
     CDS             complement(17414..18202)
                     /locus_tag="OHN28_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCQ2298385.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18295..18678
                     /gene="crcB"
                     /locus_tag="OHN28_003036"
     CDS             18295..18678
                     /gene="crcB"
                     /locus_tag="OHN28_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCQ2298386.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(18732..18941)
                     /gene="cspE"
                     /locus_tag="OHN28_003037"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(18732..18941)
                     /gene="cspE"
                     /locus_tag="OHN28_003037"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2298387.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19116..19634)
                     /gene="pagP"
                     /locus_tag="OHN28_003038"
     CDS             complement(19116..19634)
                     /gene="pagP"
                     /locus_tag="OHN28_003038"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001784638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCQ2298388.1"
                     /translation="MQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYIPAITWH
                     ARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWES
                     TWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNN
                     GNVYFAWMRFQF"
BASE COUNT         4959 a         5004 c         4931 g         4766 t
ORIGIN      
        1 aataaaaaga aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat
       61 atttatagtt gcagcaataa cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
      121 agccatggcg aattatgcct ttgattattt aagctcaccg ggcagtcttc cattcaccac
      181 ggcagccacg gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga
      241 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      301 tgagcaatca gcagaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct
      361 caaaggtctg ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc
      421 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggctcagg
      481 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      541 caaaaatgcg gaaagattac ttgccgggct ttccgcacat taacgtcgac tgtcacccga
      601 acagatggag tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa
      661 tgatgcccag acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg
      721 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
      781 ctgctgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
      841 aaggcgttga gaaagattat caggccgcct ttgaatagtt tacgatagcc gctgaatgca
      901 atgacgccac agcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
      961 tcgatctccg acaggctctc gacttgtatc gtaaagttca gtcatccgga accagagatg
     1021 tcagtcaaga aattcgtgag attgaagatt tactgtagag ataaaaacaa gggccgggga
     1081 atttcattcg gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
     1141 gggcgtctct ggaactggtc atgaccacat ttagggcata gcgtcagcac ttccggtgtg
     1201 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc taccacttct
     1261 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc
     1321 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
     1381 acgctgtcag actcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
     1441 cgtctgacag ctcgcgtcag ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt
     1501 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
     1561 tcgctcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
     1621 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
     1681 ccacaaactc tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgagtgcgc
     1741 gaaaggctgt tttgacgcgg gcgtttgggc tatgctatgc ggatctgaaa aaccacatca
     1801 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
     1861 gcccggaaga gatagaatcc aaagtacagc ttcactggga tgagaagcgc acatttgaag
     1921 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
     1981 gtcgactaca catgggccac gttcgcaact acaccatcgg tgacgtgatc gcccgctacc
     2041 agcgcatgct gggcaaaaac gttctgcagc caatcggctg ggacgcattt ggtctgcctg
     2101 cggaaggcgc ggcggtgaaa aacaacaccg cgccagcacc gtggacgtac gacaacatcg
     2161 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
     2221 cgacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
     2281 aaggcctggt atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
     2341 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgagcgta
     2401 aagagatccc gcagtggttt atcaaaatca cagcttacgc tgacgagctg ctcaacgatc
     2461 tggataaact ggatcactgg ccagacaccg ttaaaacaat gcagcgtaac tggatcggtc
     2521 gttccgaagg cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
     2581 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtggct gcgggtcatc
     2641 cgctggcaca gaaagcggcg gaaaataatc ctgaactggc cgcctttatt gacgaatgcc
     2701 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
     2761 gctttaaagc ggttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
     2821 tattgatgga atacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
     2881 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggca gctgacggct
     2941 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
     3001 agtttaatgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
     3061 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc
     3121 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc
     3181 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
     3241 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcactgcgtg
     3301 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
     3361 cggagtacaa agaaggtatg ctggattcca aagcggctaa ctattggctg ccggtggata
     3421 tttacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
     3481 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
     3541 agggtatggt gctggcagat gccttctact atgttggtga aaacggcgaa cgtaactggg
     3601 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
     3661 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
     3721 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
     3781 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggc gtggaagggg
     3841 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
     3901 ttgcggcact gaacgtcgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
     3961 ataaaactat cgctaaagta accgatgata tcggccgtcg tcagaccttc aacaccgcaa
     4021 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
     4081 atcgcgccct gatgcaggaa gcgctgctgg cggtagtccg tatgcttaac ccgttcaccc
     4141 cgcacatctg cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc
     4201 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
     4261 ttaacggtaa agtccgtgca aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc
     4321 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
     4381 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
     4441 cgtgcgatat ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
     4501 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaagtcatga tcctggactc
     4561 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
     4621 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
     4681 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
     4741 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gacatctacc cgattagcgc
     4801 caaggtcttc cgttcgttct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
     4861 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
     4921 aagcatccgt gctgcagata ttcgttccga cgaagaacag acgtcgacca caacggatac
     4981 tccggcaacg cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg
     5041 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
     5101 gatcctctgt tattgcagga aagccaggac gctgttcgtc aggtcgctgc ggcgcaaggg
     5161 ttcgaagaac accacacttt ttccattgat cccaacaccg actggaatgc gattttttcg
     5221 ttatgccagg ccatgagcct gtttgccagt cgacaaacgc tattgctgtt gttacccgaa
     5281 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
     5341 gatctactgt tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
     5401 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
     5461 ctgccccgct gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgctgca
     5521 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggccca ggcactggag
     5581 cgtttatcgc tactctggcc ggacggcaaa ctgacattac cgcgcgttga acaggcggtg
     5641 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
     5701 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg
     5761 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
     5821 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
     5881 agcgaagcgt taaatcgctt aagccagccg cagttacgtc aggccgtgca actcctgaca
     5941 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
     6001 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
     6061 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atcttaaacc
     6121 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
     6181 tcctccgcat cgtccccagc cggaagcgaa cagcatgcag cgtaaacaca tgcttgaact
     6241 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
     6301 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
     6361 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
     6421 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
     6481 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
     6541 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
     6601 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattac cggaaccggt
     6661 actgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
     6721 atgcgactgt ggttaattcg tcatggtgaa acgcaagcga atgtcgatgg tctgtacagc
     6781 ggtcatgcgc ccacacccct gaccgcgcgc ggtattgagc aagcgcaaaa tctgcatacg
     6841 ctgctacatg atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat
     6901 accgcgcgac tggttcttag tgaccgccag caccccgttc atatcatccc tgaactcaat
     6961 gaaatgtttt ttggcgactg ggagatgcga catcatcgtg acctcatgca agaagatgcc
     7021 gaaaactata gcgcgtggtg caatgactgg cagcaagcta tccccacgaa cggtgaagga
     7081 tttcaggcat tttcgcaacg tgtggaacga tttatcgcaa ggcttagtga atatcagcac
     7141 tatcagaata ttttagtcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
     7201 attggtatgc ctgccgagtc tatgtggcat ttccgcgttg accaggggtg ttggagcgcc
     7261 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
     7321 gagaatgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
     7381 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
     7441 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
     7501 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
     7561 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
     7621 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa
     7681 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgcc atgttatgtc
     7741 cattgctgat cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
     7801 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
     7861 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
     7921 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
     7981 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg
     8041 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
     8101 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
     8161 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg
     8221 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc
     8281 ggcacttacc cttccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
     8341 gtggagcatc accaccaacc atccttatca ccgtgagtga taagtgagct ttgagtagaa
     8401 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
     8461 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
     8521 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
     8581 ccgcattaag ctggtgccta tcgcacccag ccgcggcatt atctacgacc gtaacggtat
     8641 ccctctggct ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
     8701 cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat ctgaccgatg atgatattgc
     8761 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
     8821 cctgaccgaa gtacaagtag ctcgctttgc cgtcaaccag taccgttttc cgggtgttga
     8881 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
     8941 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
     9001 caactatgcg gcaacgcatg atatcggtaa gctgggcatt gagcgttact atgaagacgt
     9061 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
     9121 ccagttaaaa gaagtaccac cgcaggccgg acacgatatt tacctgacgc tggatctcaa
     9181 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga
     9241 tccgcgtacc ggtggggtgt tggcgctggt ttccacgcct agttatgacc caaacctgtt
     9301 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
     9361 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
     9421 ggtttcggca ttgagcgccg gagtgatcac gcgcaatacg acgttgtttg accccggctg
     9481 gtggcaactt ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
     9541 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
     9601 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
     9661 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
     9721 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta
     9781 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
     9841 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
     9901 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
     9961 cggtatgtac ggtgttgcta accgccctaa cggtacggcg cataaatatt ttgccagcgc
    10021 accgtacaaa attgcggcga aatccggtac cgctcaggtc ttcggtctga aagcgaacga
    10081 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    10141 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gtggcgcggg
    10201 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    10261 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    10321 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    10381 atcttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    10441 attggcatga tggagcgtaa aatcggccag attgcgatgg gtctggtcat catggtggtg
    10501 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    10561 attattttgc tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg
    10621 ctggacctcg gtattgttcg ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg
    10681 atggttgcac gctttatcaa ccgcgacgtt tgcccacctt cattgaagaa tacagctatc
    10741 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    10801 tcaatcctcg ttgcgctttc cggcctgttt gtactgttcc tctctggcct tagctggcgt
    10861 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    10921 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    10981 ggcgcaggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    11041 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccataccgac
    11101 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct
    11161 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    11221 ggtcgcgtca tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    11281 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    11341 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    11401 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    11461 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    11521 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcagaccc
    11581 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    11641 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    11701 cgaacccggc agtaacctga cggcctctgg cgaagctttc gatccgacac agttgacggc
    11761 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    11821 aatgatcgtg gtgcgcatta atgatcgcgg cccttatggc aacgaccgcg tgatttcgct
    11881 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    11941 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    12001 caaacagact tacgccctgc cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    12061 agtgcctgtc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    12121 tccgaccggc gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc
    12181 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagctccgtc
    12241 gacaacgcct gcaacctcac ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    12301 cggcaacttt atggtgcaag tcggggccgt tagcgatcag gctcgtgcgc aacagtacca
    12361 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    12421 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    12481 gcaaaccgaa gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat
    12541 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    12601 cggtagtgca tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc
    12661 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    12721 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa accatgatcc
    12781 cgggtgtacc gcagatcgac gcggagtcct acatcctgat tgactataac tccggcaaag
    12841 tactcgccga acagaacgct gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    12901 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    12961 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    13021 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    13081 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    13141 ttggcttgat gaacagctac gttaacgccc tgggcctgaa aaatacccac ttccagacgg
    13201 tacacggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    13261 aggcgttgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    13321 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    13381 acggcatcaa aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg
    13441 aaggccagat gcgcttgatc tctgcggtga tgggcggacg tacttttaaa ggccgtgaag
    13501 ccgaaagtaa aaaattgcta acctggggct tccgtttctt cgaaaccgtt aatcctctga
    13561 aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt
    13621 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    13681 ctagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    13741 gaactatcaa cttccagctt gacggcaaaa cgatcgaaca acgcccgctg gttgtgctgc
    13801 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    13861 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    13921 gcatcagtaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    13981 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    14041 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    14101 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    14161 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    14221 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    14281 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    14341 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    14401 gccaatctcc caggctatgc atgaattcac cgatacccgc gatgaaagta cccttgatga
    14461 aatctggctg gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca
    14521 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    14581 tcacgggccg gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    14641 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    14701 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    14761 ctcactgggt ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    14821 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgcaggaa tggaaatggc
    14881 taaaatatca caatggaaac ccgaagcgac gactaataat attgcaccac gtttactgga
    14941 aaatatttta gcgctactaa acaatcctga cttcgaatat attaccgctt aattccatac
    15001 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    15061 cttcgcgttt gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt
    15121 agcccaccta attgattaac tcattgaaat aattgaccat ggtgagcaaa agcgttccgt
    15181 cccaattaga cataagatta ttgtaatttt tttaaatatt attttttcat gaataaaatt
    15241 ggagtaagtg cgtggatagt aataataaaa ttgaaccctg tttaagcaga aaatcgtcag
    15301 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaccctt ctgactattt
    15361 tgaagttgca tatgtccata aaggaatcgt taatgcagcg aaagtgctta atctgccccc
    15421 cctcggcaat ctatcagcct attcagaaac tgcgcgttat attccctgac ctattgttta
    15481 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gtatctatat gagtctatca
    15541 ctcagggact tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    15601 ggacgataac tattgcgccc tccccctcgg tcggagccct ggtccttcct gtcatctatc
    15661 gggcgattaa aactcactat ccacagcttt tactgcgcaa cccgcccatc agcggcgcgg
    15721 aaaaccaact cagtcagttt caaaccgatc tcatcatcaa taacatgttt tgcacggggt
    15781 catagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcac
    15841 aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt
    15901 tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa
    15961 caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga
    16021 gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta
    16081 tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga
    16141 aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa
    16201 atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgctttaacc
    16261 acggcactgc aacgtttatg atcctcggcg ctatttgtac ccgccgttgc ccgttctgtg
    16321 acgttgccca cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga
    16381 ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgatctgc
    16441 gcgatggcgg tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc
    16501 aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg tatggatcgt gctctggata
    16561 ttctgactgc aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt
    16621 accgtcaggt acggcctggt gcagattaca actggtcgct taagctgctg gaacgcttta
    16681 aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggactg ggtgaaacca
    16741 atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgctaacgc
    16801 tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg
    16861 atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg
    16921 gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt
    16981 aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt
    17041 tgtatcgaac aagatattga gggagcgtcc tgctcgccac gtcactcttt atgagagagc
    17101 ttttcagcct gaagatcaac gtctgcgcct tttttcgccg cagcatcgtc atcattcatc
    17161 gccttcttga accctttaat ggccgctcca aggtctccgc ccagcgtacg taacttctta
    17221 gtcccaaaca gcagaacgac cagcgccgca actaccagca gtttggtaat actaatctca
    17281 cccatagata ccttcttgac atataaccgg caacagaacg cgccttaaaa accagagaat
    17341 attaacggtc attcgacgcg cgcacacaat agcaatctgt aacaaggtga atcaagcgcc
    17401 gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg acggttgttt aagacgggca
    17461 gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc cgccataatg agtgcaggca
    17521 tttctgacgc tgccgcaatg gtgacgccaa agggatctat aatccggctt tgcccgatat
    17581 ttttgttccc gcactccccc gccgccacca tataacaggt ggtatccagc gcacgagcgg
    17641 caagcaacgt tgaccagtga tgctctttga gcggcccacg aacccaggcg gcaggaagta
    17701 ccagtatttc agctccctgt aatgcctgtg ccagcgccag ctctggaaag cgtaagtcat
    17761 aacaggtcat cagaccgacc ttcatcccct ccacctccag taacggagct atttcattac
    17821 cagcatcaac atggcgtgat tcctgaatgg caaatgcatc atagagatgc agtttggcat
    17881 aacgggcgac gatgtttcct gcctgtagcg ccaccagcat attccatgcg cgccccggtg
    17941 ttgaaggaac atgaatcgtc agaattgtcg tcatcatgtt acgtttactt tctcgccgta
    18001 aacgaccgag gaattcgcct tccagcagtt gtgctgattt aaccgataga tctgcatcat
    18061 gatcatcacg cgccagtaat gcttccggca ggacaaacag cgaaacctcg ttttccgccg
    18121 cctgcgccat caacgaggca caaatctcaa cgttcttttc ccacacagat gtaacagcaa
    18181 actgtcctgc ggcaaccagc attgccacct cctgtcagag tcgtaaaatt cgccaacggc
    18241 gattattcgg cgttacactt atcactcata caaatcaaat agcaggattt tgcagtgtta
    18301 caacttcttt tagcagtttt tattggcggt ggtacgggaa gcgtggcgag atggctgtta
    18361 agtatgcgat ttaacccgct gcatcaggcg attccgttgg gaacgctggc agcaaacctg
    18421 attggggcat tcatcatagg aatgggattc gcctggttca gcaggatgac gaacattgat
    18481 ccagtgtgga aagtattaat caccaccgga ttttgtggcg gtctaacaac cttctcaaca
    18541 ttttcggcag aagtggtgtt tttgttacaa gagggccgct ttggctgggc gttactgaac
    18601 gttttcgtca accttttggg gtcttttgcc atgaccgcac tggctttctg gctgttttcc
    18661 gcctcaaccg cacactaaag accaaaaaaa acccgctgat taagcgggtt ttgaattctt
    18721 gctgacgtat cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg
    18781 atttcgaact ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca
    18841 gagaagtgta cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg
    18901 gactcattaa accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga
    18961 aaaatagaca caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag
    19021 tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt gatgttaatt attttagtgg
    19081 ttgattttaa agaatttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca
    19141 aagtacacat tgccattgtt gtaggtaccc ggaatgtagg tcatctgaaa cgtcgcgggg
    19201 ccatacccca cagaggccaa tggcagtaga accggaagag ggatgtaatt ccagttatcg
    19261 cgcgccgtta cgccagcggt aaatcccagg cctaaatgaa aattttcatc cgccagcggt
    19321 cgccaggtac tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta
    19381 aatgccattg catacaggcc atgccagttt cctttttcat cccaacgcga ctgaccaaaa
    19441 ccgccacccc acggtcgctc gttatagcga tcggtttttt ctttgtcata ggcaaaacgc
    19501 gcatgccagg tgatggcagg aatatataaa tcataatgtt ctggctgttg ccaggtttgt
    19561 gcaatatttt ctctaaacgt tgtcatccac tcatcagcgt tagcaaaaac tttaccaaca
    19621 ctgattaact gaataaaaac aaagaaaaat atagcgacat
//
LOCUS       DAJOTB010000087        19576 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJOTB010000087 DAJOTB010000000
VERSION     DAJOTB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.087"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..511)
                     /locus_tag="OHN28_003039"
     CDS             complement(<1..511)
                     /locus_tag="OHN28_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2298389.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSAENSGNQVIFTLYVPPDTGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVVYDNYVTPEEADKKYLQISKNL
                     SEIPANGETA"
     gene            complement(504..1130)
                     /locus_tag="OHN28_003040"
     CDS             complement(504..1130)
                     /locus_tag="OHN28_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCQ2298390.1"
                     /translation="MTSQSVLPPNAITPERALEVVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPRDDPYTFAIKIYLLKSMGLDLELLNTFIAQIFDAKNCRSLLKEINFETGVDG
                     EFFIAGTPYAKIGVNVPADGDGGVKINGGLYISGSPIVSLNVEIGPNG"
     gene            complement(1133..2053)
                     /locus_tag="OHN28_003041"
     CDS             complement(1133..2053)
                     /locus_tag="OHN28_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCQ2298391.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKGSDLDQIGANFDCL
                     RLLITPANPDVIPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGTPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLAIYVDTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDVIAGVGQAPYCTEIKLSVQMG"
     gene            complement(2050..2391)
                     /locus_tag="OHN28_003042"
     CDS             complement(2050..2391)
                     /locus_tag="OHN28_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCQ2298392.1"
                     /translation="MLGMDRNTGKLLSGTDHIRQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            complement(2394..3296)
                     /locus_tag="OHN28_003043"
     CDS             complement(2394..3296)
                     /locus_tag="OHN28_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCQ2298393.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDDKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVQDRQGTITEV
                     RTTYNGHTHICPDGETQQPNQPMA"
     gene            complement(3277..3813)
                     /locus_tag="OHN28_003044"
     CDS             complement(3277..3813)
                     /locus_tag="OHN28_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298394.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDESVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWINGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHEGDYYGPIGR"
     gene            complement(3810..4490)
                     /locus_tag="OHN28_003045"
     CDS             complement(3810..4490)
                     /locus_tag="OHN28_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298395.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMVYNRALNRTA
                     KHMHRISAGMILTALAAKNHKAVDKRIKPFIKRRNFTKEGAGDLSSAKLWYGLNDFRV
                     SELKGRLQNPRRQKQPRDLETGLFLKTKKGARGATFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLDRGGIEEARVPVHDALEDAIDDYIFENIGPVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            complement(4522..4902)
                     /locus_tag="OHN28_003046"
     CDS             complement(4522..4902)
                     /locus_tag="OHN28_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298396.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIEPDGSVTWSGWTDYVVKEPQP
                     DGTGLTNIFLEPHTSSENSEYSKY"
     gene            complement(4899..5318)
                     /locus_tag="OHN28_003047"
     CDS             complement(4899..5318)
                     /locus_tag="OHN28_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCQ2298397.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNTASGTDNELAQSIASAIVSQSQEQELAPATRRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            complement(5353..6387)
                     /locus_tag="OHN28_003048"
     CDS             complement(5353..6387)
                     /locus_tag="OHN28_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCQ2298398.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYLDPVTDTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGISEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            complement(6446..6775)
                     /locus_tag="OHN28_003049"
     CDS             complement(6446..6775)
                     /locus_tag="OHN28_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCQ2298399.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATVG
                     TFKVWDGSVGKAIGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPISVG"
     gene            complement(6775..8082)
                     /locus_tag="OHN28_003050"
     CDS             complement(6775..8082)
                     /locus_tag="OHN28_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCQ2298400.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPTYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYNGIAKRLQQAISDPDVRGVLLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPGDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKMERFMSGTETTAETTTTEQTATTTTVAQVEFNTEQIR
                     ADAASSELARVMAIINCPEAVGREAQAKALAGVPGMTVGQAQVVLAAAPKTAQARTET
                     ALDTLMSTESPATIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            complement(8082..9656)
                     /locus_tag="OHN28_003051"
     CDS             complement(8082..9656)
                     /locus_tag="OHN28_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2298401.1"
                     /translation="MTAPQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSAD
                     AALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYLGISREDA
                     RALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESSAG
                     SVFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCR
                     RIPAQLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMY
                     AATIESELDSQQAFEYIAGAGTDIDSNPLNSFIQSYVTYYNGANIKLGGVKVPHLHPG
                     DKLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWR
                     FFMGRRRFIAARQASLMFCCWFEEALARGIITLPRSAVRSFYEARNSWTNALWIGAGR
                     MAIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSW
                     SVSHPSNTNDNGWGGN"
     gene            complement(9653..9886)
                     /locus_tag="OHN28_003052"
     CDS             complement(9653..9886)
                     /locus_tag="OHN28_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298402.1"
                     /translation="MTSREVLTERLLEAEIALHKLLTGKSTVSLSHGDSAGNNRSYQY
                     SQASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            complement(9886..11748)
                     /locus_tag="OHN28_003053"
     CDS             complement(9886..11748)
                     /locus_tag="OHN28_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCQ2298403.1"
                     /translation="MTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTNDAIKI
                     VSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAEMPVI
                     QKIFPDWDKSNERNNLQRKEMSGAILDFRGASAPGNFRRLTKQVVEGDEVDGWPLEVA
                     KKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYLTCPH
                     CGEEQVLVFGFDGIEYGLKWDNSLQTNEAKSSSAYYQCCHCPEHFYYRDLEKMEFGGR
                     WIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKAKGDP
                     LKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYITGGIDSQTSGRY
                     ECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSRWAWD
                     TGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTDTAKD
                     LLAMRLQLEPDSKSATPGAIHFPNDDEIFSTTEAKQLVSEVLIPKLINGRVVYRWDNQ
                     GRRNEALDCWVYGLAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS"
     gene            complement(11735..12301)
                     /locus_tag="OHN28_003054"
     CDS             complement(11735..12301)
                     /locus_tag="OHN28_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298404.1"
                     /translation="MASQAEVAAHLLLSDRRLRDLAKLPGAPVPQGRGDWELDAWRHF
                     YIRYLRSNRRDTTSNDEPEAGDNSPEKNREQWLKNEERQERILMARVKRRILAKRYAP
                     IELISVAVSRVAVELRTRVESWPPRLKKVWPEMPQEASSVLREELAIALNELSDIRID
                     FSDYDVSDIERDLDRVESLARDDTDDGC"
     gene            12670..12915
                     /locus_tag="OHN28_003055"
     CDS             12670..12915
                     /locus_tag="OHN28_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298405.1"
                     /translation="MNREKIEALMSVILKEADPKKFPEKLSEDAIVSFYRANSSYLNC
                     STLREWLSGEAEPSDEILQKIMVQNNWRATKWSNQLP"
     gene            complement(12975..13169)
                     /locus_tag="OHN28_003056"
     CDS             complement(12975..13169)
                     /locus_tag="OHN28_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298406.1"
                     /translation="MKKLLLAVAFFTLAGCTHSTYTEATRADGSSIKHVMIAPGTKIT
                     TANGGCIDSTGAEASCLGGK"
     gene            complement(13177..13656)
                     /locus_tag="OHN28_003057"
     CDS             complement(13177..13656)
                     /locus_tag="OHN28_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HCQ2298407.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERAGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADEVNIKAFPRSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            complement(13656..13928)
                     /locus_tag="OHN28_003058"
     CDS             complement(13656..13928)
                     /locus_tag="OHN28_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCQ2298408.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRIFYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            complement(13928..14311)
                     /locus_tag="OHN28_003059"
     CDS             complement(13928..14311)
                     /locus_tag="OHN28_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298409.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            complement(14424..15095)
                     /locus_tag="OHN28_003060"
     CDS             complement(14424..15095)
                     /locus_tag="OHN28_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCQ2298410.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMSDFEFADSSWRRSVNSLDASQQ
                     AWLRYCYGGNLAFKHQTAICEAVWSRYKGNIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSTLAGMLSVSRSTWCETYSLHWVGMKEAVRALDEVALLATLHHYQNHL
                     DDVCV"
     gene            complement(15095..15388)
                     /locus_tag="OHN28_003061"
     CDS             complement(15095..15388)
                     /locus_tag="OHN28_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCQ2298411.1"
                     /translation="MKKIDLRKAARSRACTVRIPGVCNHNPETSVLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEHDSLLLMHAEGVFRTLAIWRDEEFI"
     gene            complement(15385..15981)
                     /locus_tag="OHN28_003062"
     CDS             complement(15385..15981)
                     /locus_tag="OHN28_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCQ2298412.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPAERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGSISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            complement(16059..16238)
                     /locus_tag="OHN28_003063"
     CDS             complement(16059..16238)
                     /locus_tag="OHN28_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298413.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
     gene            complement(16390..17031)
                     /locus_tag="OHN28_003064"
     CDS             complement(16390..17031)
                     /locus_tag="OHN28_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298414.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTVVAGHPEIDVSELDIGPLYRYYKRGEKGNPLADLLIELAPPTCERVRISPEVY
                     IIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLIPIYRSDK"
     gene            17275..17508
                     /locus_tag="OHN28_003065"
     CDS             17275..17508
                     /locus_tag="OHN28_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298415.1"
                     /translation="MGTLGNQPCRSRYEFDDFNLANRAAALTKLAKETGVSVDTLIKL
                     EIAMQLNRLNSILIENGDKMDENLAGIGEILQK"
     gene            17907..18395
                     /locus_tag="OHN28_003066"
     CDS             17907..18395
                     /locus_tag="OHN28_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCQ2298416.1"
                     /translation="MATNPREHARRVLDTFWCGRGFPVDPAKIAHDMGLDVFITDLPG
                     KVSGALIKQKDQDPAIFLNSDDNKVRQRFSCGHELGHYIARQASHSDEYEYVDLRGET
                     ASAGTHPDEIFANKFAAELLMPVDEVRKLHADGQPSYIMAHYFGVSDDAMTYRLKNLR
                     LG"
     gene            18405..19010
                     /locus_tag="OHN28_003067"
     CDS             18405..19010
                     /locus_tag="OHN28_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298417.1"
                     /translation="MPSDDDFNSRFKQEVARRLKDSLNLHRQLAEEVEPEDDETPVQS
                     PDEASPQPKVSHAPKKTAVRIRGIARKRAAEARKMAVAASSKRIPTATPSQQVTRTES
                     KILTEFTSVKQVNSLDTEHERKQLNNKDFEAEIALKKRYGKWFLIILAFQLLIMNGIF
                     IADGAGKLEFEDLTLQLYMGGTLTEVFGLVLVVTKYLFKRK"
BASE COUNT         4764 a         5128 c         4620 g         5064 t
ORIGIN      
        1 gagcagtttc accatttgcg ggaatttcag ataagttttt acttatctgc aaatattttt
       61 tgtcagcttc ctcgggagtt acgtagttgt catatactac aacattaacc gtagatgtgc
      121 tggacaccgc gagtgtgtat ttatacgtga ttgaaatcag cgcaccgttg ctgtccgtag
      181 gttttaaaat gtccggcgag cgcgccacag agtacagctc gcctttatct gttaatatcc
      241 ccacctcacg gatagtatag cccccagtgt ctggcggtac gtaaagtgta aagataacct
      301 ggttaccgct attttctgcg gatttaatat ccccacgata cgtctcgtga attaatgcag
      361 ttcgtgccgg gtccggtgtt acgtcgtcgt ttccgtttgc gtcgccaata acgaatttat
      421 tcagcaccac gggtacaccc gatgccagcg cagcggcttc aagttgtgcg ccacggtctg
      481 tcagtactgc gtaaatttta ttttcagcca tttggcccta tctccacatt taaactaaca
      541 attggtgagc ccgatatata tagcccgccg ttaattttta caccgccgtc gccgtctgct
      601 gggacgttta cgcctatttt cgcgtaaggt gtaccggcta taaaaaactc accatcgaca
      661 cccgtttcga aatttatttc ttttaataag gaacgacaat tttttgcatc aaatatttgt
      721 gcaataaatg tattcaatag ttctaaatct aaccccatgc tttttagcag atatatttta
      781 attgcgaatg tatacgggtc gtccctgggc gtggcttcaa accactctct cattttgctc
      841 tcaaagccaa cagttaataa cgctcgttca acagctccac gcgttccgcg gtgcttattc
      901 tgccacgcag ctgctttaat aatttcgcgt ttttgctgct cactccagtc ggggttccag
      961 taggtgaccg cgtactccca cgccagccac ggcaacagat ttgcagggca caaatcggga
     1021 tttttaataa ttcgaatatc gccaggcaga tcgccgacgt gagataaaac aacctcaagc
     1081 gccctctcag gggttattgc gttcggaggc agtacgcttt gacttgtcat ggctacccca
     1141 tttgcacgct gagtttgatt tctgtgcagt acggagcctg cccgacgcca gcgatcacat
     1201 cctctgttgg cgatatcaga atcacacgtt cagtgccgtc acgatgtatc gcgtcgtaaa
     1261 ttcctgacag agctgccgtc gctccgattt tatgttgcgt atccacatat atcgccaaat
     1321 cgctctgagc accgttaagc aaaacgttct ggtcgggtcc gagcccggct acgataacgg
     1381 cctcaacccg gtaattcagg ttcgacgcag gttttacagt cacgaaatca gtcagcggtc
     1441 gagtatcgtc aggagacagg gccgcattca ctgtattcag tagtgattga ggcggagtgc
     1501 cgtcgccggt tcgcgacaag acatacatat cgacgtatcc aggctgggtc accggcgggc
     1561 cgtacgcctg agctgacaga acgtcgggat cactcgacag cgcgaaatag ttataggcgt
     1621 tcgtactgcc ggcagtattc cgcgcatacc atgatagctg tattcggtgt cgatactcgt
     1681 cgtcactctc gtaaacagcc tcagtcgggg gaattacatc gggattagct ggcgttatca
     1741 acagccgcag gcagtcaaaa ttagcgccaa tctggtcgag gtcactcccc tttgcaaacg
     1801 cgagcaatac agcgagcatc ccctgattta cccgagctgt aataatgatt tctctgtatg
     1861 caactatttc cagcagttta acggccgggt ctgactccag gagtgcgtta aattgtggat
     1921 ataatatctg cagctctgac agtagattag atttaacatc tgaaaataac ggggttttaa
     1981 cgaatgctgg cggcggcagc agactcatat caatagtttt cactatcgaa ttctgaactg
     2041 ttgatgttat caaaatatca gctccatatt attaaactct aatcgctgtt gtgtttcgat
     2101 atctgttgcg ataattaata ttgttatttt cccgatatcc actgctaaca catttattct
     2161 gtcaattctg acgcgtggtt cccatcgtgc tattgcgcca gcgctttcca tgattattcg
     2221 catagcaagc gacggatctg tgggattatc aacaagatca aataatttgc tgccatattc
     2281 cggcagcatt acacgcgtcc ccagcggggt cgttaaaata tcaacgatag actgacgaat
     2341 gtgatcagtc cccgataaaa gtttaccggt gttacggtcc attccaagca tctttacgcc
     2401 attggttggt tcggttgttg agtttccccg tccggacata tatgagtgtg tccgttatac
     2461 gttgtgcgga cttcagttat tgtgccttgt cggtcctgca cctcttgccc tgctaacaca
     2521 ctaccgcgag ttgtgatatt acctgttcca ccgttatctc cagtgacaga aatatcgtta
     2581 cggaatgtcg ctaatttatc tactactaat gtctcggtaa tatgcgttgg accagctagt
     2641 gttataccac ccggagccca cgcgctgatt gtttgagctg tcagcgatat tctgttcgca
     2701 gcagtaacac taacagaact cgttgctata acgttcacta aatcagcatg tgcgttaatt
     2761 gtatctgtcg ctgtgacatt aataatatca gcgtctgcgt taattaattt cccaccatgg
     2821 atattaacta cattttcgct attaatagta acatctgcgc gtccaaatat ttctatttca
     2881 gactcaccga gtatttttac cacgccgtcc ggtacgccct gccatgtcag agtgtgattt
     2941 tttgtgttat acgtcatcgt agccccgtca cagtatgcag tgacatgatc atgggggtca
     3001 ttgcttggcg gagctttgtc gtcaatgtgc agcccgagca taactacacc gttccgcgta
     3061 tccccaccct cagaaacaac agtaacggga tcgcccactg atggtgcgct ccagtcgacg
     3121 cgttctgagg tggcgtgagt aaaccactgc agccagcctg actggtgttc gccaccaaat
     3181 gagactcggc agcgtggagg attcatttta actgcagcta tcgtgccgcg tttaactgcg
     3241 tcacgcagcc tgcgcgcgtt ctccgctgca gcatattcat cgtccgattg ggccataata
     3301 gtcgccctca tgttctgcgc cgatatctgg tgctattccg acgtatatat ttttcagggt
     3361 cccctgtgag ggataatcga aaatatctgg accaacgcct acaacctgcg tgaacgacac
     3421 gcactgaact gaatgcgaac cgactgactg cccgttttta atccagtcgc acggttccgc
     3481 gtcgctgaat tttgccggtt tagttccggg accgaacatt cgcccattga tccagctcgt
     3541 catatagagc gctgcattct gcgatttcag tccgtactga tcagctgcaa attcacgcag
     3601 caaatacaaa ttacacgaca gctcgactga ctgaattgag cctggaaccg attcgtcact
     3661 ctgtgaccag ttgctgattt ccaggaacag agctggcgtt tcaaatccag ccggaatttc
     3721 cggataaatt ccgaatgttt tgatgaacgg tatttgtaaa accgcgcctt taacgcgatc
     3781 aagatattcg tcaaatgcat ccagtccgct cataatcgtt tacctgtttt tgggtctacg
     3841 tggacattac ctttcacgcg accgcgcaaa tctttctcaa aaaagcccat aaaaacaggg
     3901 ccaatgtttt caaaaatata atcatcgata gcatcctcca gcgcgtcgtg cacaggtacg
     3961 cgagcctctt cgattcctcc gcgatccagt cgaatccaga cacttttcgc accgtagcgt
     4021 ttcgctacga atgaatcggg ccaactcatc atcgccagcc ctgcactttt gggtgtaaaa
     4081 gttgcccctc tagcgccttt tttggttttc aaaaacaggc cggtctcaag atcgcgaggc
     4141 tgtttttgcc tccgtggatt ttgtaatcgc ccctttagct cagatacccg gaaatcattc
     4201 agaccgtacc agagcttggc gctgctcagg tctcctgccc cttcttttgt aaaattacgg
     4261 cgcttaatga acggcttgat tcgtttatct acggccttat gatttttagc tgccagtgca
     4321 gtcaaaatca ttcctgctga aattcggtgc atatgttttg cagttcggtt cagcgccctg
     4381 ttgtatacca ttaacatttg atgttgcgtc gcgctgattt cgactctgag ctgctccagc
     4441 gccgaaacat cgatatcgaa catctgcgcg ttactacgta aatcggccac ggttccccct
     4501 taagtgggac cgaggtccca cttagtattt tgaatactcg ctgttttcgc tggacgtgtg
     4561 aggctctaaa aaaatattcg tgagccctgt gccatcaggt tgaggctctt taaccacgta
     4621 atcggtccag ccagaccagg taacagagcc gtcaggttcg atgctgcgct gctttggaac
     4681 ctggacgacg tcacgcgcaa caacgccagc gacgtcgtcc gagagcgccg tcaggctcgt
     4741 tactgtaccg gtaataaaac cagcgtgagg aacgtcaacg cgcgcgtaag gctcgttgaa
     4801 aatagcgata atggggtcgg ttttaccgcg taatttaacg ggccgtcccc actcacggat
     4861 catctcctcg tcgccggccc ggaggtcatc gtagtagctc atagagcgta aacgtgatca
     4921 gctgcgataa ggtctgcgac ctccggtgaa tcaacaacaa tttcacggcc tgcggcaaca
     4981 atctcgcgaa cgcgacgccc gttcacgtag tgataaacgt ccagcgtgtt acgcaacttg
     5041 acgcggcgcg tagccggtgc gagctcctgc tcttgtgact gtgaaacgat agcgctcgcg
     5101 attgattgag cgagttcatt atcggtcccg gacgcagtat tcactgtaat ctcgatgcct
     5161 cccgcgtcac cggattgctc gttcagaata tcgagctctg actcagcgcc ggcaacgatt
     5221 gattccagtt ccgcaatagt cccgctgcgc ggtaattcgc gaccgagctg agcgctcaga
     5281 tcgtcaatac gcgccagcaa ttctgttttt gtgctcatgt tgattcctta acaaaaaaga
     5341 gcctttcgac tcttacgcga tttttacgac aacgaacgcg tcggcgtccg tgaggaccat
     5401 tgctggcgcg gattgcgtca ttgtttgcgt cactgccgga tcaccggtag tggtccagac
     5461 ttttggatag cgcgtcgctt cggaaatacc ctctttcagc gcgtcttcgt cctgaattgc
     5521 gccataagtg cggagcccgc ggttttttgt gttgccaagg atcatcgtgt tatccggcat
     5581 ataacgcgtt tcagtgtctg ttaccgggtc gaggtattgc cctttgtaga caaacagcgc
     5641 agtgtcgccg tagtagcctt taaagctgac aacgtcgccc aggtttttca gcgcgacctc
     5701 gagctggctg tttgagccac ggcgcgtatc cagtgccgtc cagaattttt taaaacgctt
     5761 caactgcttc caggctttac catccatgat gatcacgtta accgcaccgg aggcgaggtc
     5821 cgcgtaggat tcaatatcgt cgctcgggtc atacgtttcc gcgtcctgag tcgaccaggc
     5881 tgtcgaaccg gactgagtga cgttattcgt cgcgctgcgg ctcatgtcga tctcataggt
     5941 ctcgatattg ctgccggaaa cagtgtattt accgtacaga acagcctgta ccgcctggaa
     6001 ctcttcgagc tggctgatgc tcagttcctc atcaagcagg ttctgcatga tgatttttgc
     6061 acgacgttcg accggcgttt ccggctggcc aatctgctca ccggctgcgc gcttgatgct
     6121 catattcgga ttgatcgtgt gcttgggttt cgtgtaaccc ggtttgaaat ggttcgttga
     6181 atagccgcga gtgcggtcaa ctttgccggt gatcatcggc gcgcagtaca cggccatgtt
     6241 cacttttccc gggattttat ccagaaaaac ctcttcgctc gtaaacgtgt aggtctcacg
     6301 gaaaaacagc tttaaaaaca acggattgaa cttaaatacc tgctgtgtcg cggtaatcag
     6361 ttctgacgta gtaaacgaat cgctcattta tatatcctgt tataaaaaaa ccgcccggag
     6421 gcggcttgtt ttgagttttt tgctatcagc cgacgctgat cggcgtaccc gcaaatgctg
     6481 atttgcgttt agctacgtcg gtaaccgtac cccagttaat cgccgtgtaa cggaacgagc
     6541 ctgatttata gtacgagcag tctgcagaac tggagccagt attaactgcg gtggcagtga
     6601 ggccaatagc tttaccgact gagccgtccc aaaccttaaa cgtcccgaca gtagcgtcga
     6661 gcataatcgg tgtcagccgg ggtacgttga tcccgccctg aaaatgaccg acggtcgtga
     6721 caacaaggtc aggcccaaga ataaaatcgt ccgggctata cgtttctgta gtcattattc
     6781 atcccctaaa attgaacgcc ctgcagcaac cagcatcgag acgtttgcgg ttgttcctgt
     6841 tgccgtagta ctgccagcat cctgaatagt cgctggtgat tcagtgctca ttagtgtatc
     6901 gagcgccgtt tctgtccgcg cctgcgccgt tttaggtgct gcagcgagga ctacctgcgc
     6961 ctgcccgacc gtcatcccgg ggacgccagc gagcgctttt gcctgcgcct cgcgcccaac
     7021 agcttcggga caattgatga tggccatcac acgcgccagt tcgcttgatg cggcgtccgc
     7081 gcgaatctgc tccgtgttaa actcaacctg cgcgacagta gtcgtggtcg cggtttgttc
     7141 tgtggtcgtg gtctccgccg tggtttctgt accagacata aagcgctcca tttttggttt
     7201 cagtgcgtcg gccatcactg cgatagcgtc cgcgtaatta acgagttgat cagcgagacc
     7261 agatttaatc gcgtccgcgc cgataaatac tgcagcttcc gtggccagca ccctggattt
     7321 tttcagcccg gtataatccg agactttttg cgcgaactgc tcgcgcgtgc tgttaatact
     7381 cagctggaat tcgtcgcgaa cgtcgcctgg cagctgggaa tacgggttcc cgtcaacttt
     7441 gtgcgcgccg gcgtaaatca gcgtcacgtc gacgccagca atctccagcg ctttttcgac
     7501 gcagcggtga gccatcagga caccgattga accaactgtg cctgtctgcg ttatcagccg
     7561 gcgcgaacac gctgacgcca gcaaataggc agcgctgcag gccgtatcgc tggccagcgc
     7621 ccacacaggt ttttgctctc gcgcccgggc gattaaatca gcagtatcaa acgcgccggc
     7681 gacttcaccg ccaggggagt caatatcaag caggacccct cttacatcgg gatcagaaat
     7741 cgcctgctgc aggcgttttg cgattccgtt gtaaccgctc atcccgctaa ctgggttgat
     7801 ataacccaat ttatgaacca gcgtcccggt aaccggcaga acggctatac cacgctccac
     7861 gcgatacgat ttctggcgtg ttcgctcgct gctgtcccac cccatcgcga gcgcgttcat
     7921 ttcgtcgctg ttcattacct ctccagacgc tgtatcaatc agtcgcccgg taccgaaccg
     7981 gtcgctcagc gccgaaaaaa atacccgcgc gtaggtgggc tccagcaaaa gcggttgatt
     8041 gaacgccctg gcggcgaggt gcggaaaatt gttccacggc atcagttacc tccccagccg
     8101 ttatcgttgg tatttgaggg gtgagataca gaccatgacg gttcgctcag ccccatttca
     8161 cgccggcgct gaatttcgta ttcctgctgc tccataacct cctcgtaatc ctgtccctgc
     8221 agcgccagtt cgttctgata cgtgctgagg ccagttgtga tgcgcatggc gctctcctga
     8281 acctctttca gcccatcaat cgccatgcga ccagcgccga tccagagcgc gttcgtccag
     8341 gagttccgcg cctcataaaa cgaccggaca gctgagcgtg gcagggtgat aattccgcgt
     8401 gccagcgcct cttcaaacca gcagcagaac atcagcgacg cctgtcgcgc ggcgataaaa
     8461 cgtcgacgcc ccatgaaaaa tcgccagctg acgttagcac tggcccgggc gctggagtaa
     8521 ctgacctggc tgtagtcccg gctaagttct tcgtatgacg cgccaacgcc agctgcaacg
     8581 taacgcagga gtgatttttc cagtgaacta aaaccagcat cagcattctg agcggtctgt
     8641 aaactgagtt tgtcgccggg atgcaggtgg ggaactttca caccaccgag cttgatattt
     8701 gcgccgttgt agtacgtcac gtaactctga ataaacgaat tcagcgggtt cgagtcgata
     8761 tcagtacccg cgccggcgat atattcaaac gcctgctggc tgtcgagctc tgattcgatt
     8821 gtcgcggcat acatcgcctt cacaatggcg ctctgcagtt gtgtttgctg cagtgtgtcg
     8881 agcattttca ggcgctccat gacgctgtaa aaaatattgt ccccgcgggt ctgtccgtcc
     8941 tcgagcggct cgaatatgtg gatgaatgcg tgtctgccgc tgctgagctg tgctgggata
     9001 cgccggcatt taccgactcc gccgagaggg taagtatcct cggctatcca gtacccgacc
     9061 gcggccccgt ttttatcgat atcaacaccg gcgcggcgaa attgagtgtc agctgcgtaa
     9121 cccggatttc gaatccgttt cggtgagacc attttgaatc gcgtccgaaa aacgctgccg
     9181 gcactactct cccagactgg ttgtacgcag gtttcaccgt tgaaagcgtg agtagccacg
     9241 ccctcgcgga tcatcattgt gaacgtccgt ttccgctcga tatcaatcgt gcagtgaggg
     9301 tcctcggcgt attcagtcca cgctacctcg acgtcacgcg ccagcgccct ggcgtcctcg
     9361 cgagatatgc cgaggtaacg ccagttaggg cgataactca acttaaacag attgccgacg
     9421 atatgatcct ggtgcagctg cacggcgttc gacgcgacgc cgttattgcg aacgagatcg
     9481 tctgctcgcg cgttaccgcg ataaaaattc ggcaacagtg ctgcgtcggc gctctgctgt
     9541 ggtgcgttcc agtcaatcag ctggccaccg aaaccgggac cgccgccgtt ataccccgcg
     9601 taacggcgta gtggcgtttt accgtcaggc ccgagcagct gctgcggagc ggtcataatc
     9661 gaactcccac gggccgacgg cgtcccgtac tcagaccgag ctgtgatttc agctcgataa
     9721 tgtacgttcg gagctgctca atactcgcct gcgagtattg atagctccgg ttatttcctg
     9781 ctgaatcgcc gtgagacagc gacacggtcg atttacccgt taataatttg tgcagagcga
     9841 tttccgcttc cagcagtcgt tctgttaaaa cctcgcgcga cgtcattagc tccctccgag
     9901 cattctggcc atctcctcga gagacagttt gttttgtgat tttttccgtt gctcagcgag
     9961 cgtctcgaga ttgagctgga aacgaatttt actgatacgc agcgctgcca gtccgtacac
    10021 ccagcagtcg agcgcctcat ttcgccggcc ctggttgtcc caacgataaa cgacgcgacc
    10081 gttaatgagt ttcgggatca gaacctcgga gacgagctgt tttgcctctg tcgtgctgaa
    10141 tatttcgtcg tcgttgggaa aatgaatcgc acctggtgtc gccgatttgg agtcgggttc
    10201 cagctgcagg cgcattgcga gcaggtcttt cgctgtatct gtcccaatca acgacaaata
    10261 cactttattc gcgttacggg tacgaggcat atttacgacc ggctggccgt atgagctcgc
    10321 gcccttgatt ggaatgaccc acagcggacc gagtttcagc gagcggttat aaacgacctg
    10381 cgcatctata ccaccggtat cccacgccca gcgactgacg ccgattgtgg tcccgtcgct
    10441 gcgccggtat tgtttgcgaa tcactccgtc gacgcgctgc agcgtgtcct cctcgtcgta
    10501 gcgaccgagg acgattgttt tatcaatcag ccagcactcc tcctcggctc cccagcccca
    10561 cacataacac tcgtagcgcc cggacgtctg agagtcgatg ccgccggtta tataaacaac
    10621 accgtcagga acctggctgg cgtatttttc ccggcgatta accagaatat cgtgctccag
    10681 ctgctcgctg gccacgtcgc tccagagttc gccgagcgtc gtgttatgaa acgtcttttc
    10741 cttgagcggg tcgcctttcg ctttcagcca ctcgctgaca atctcgcccc atccatcgag
    10801 gtttagtgaa tacagggcgt ttatcacgat cgcagcgtgt ttcggcgcgc gaacgacgcc
    10861 accgtcatga tcaaaaaaat gaatgccgtc tcgggtccag gtgcagtcct cggctatcca
    10921 gcgccccccg aactccattt tttcgagatc gcgatagtaa aaatgttccg ggcagtggca
    10981 gcactggtaa tacgcggacg acgatttcgc ctcattggtc tgcaggctgt tatcccattt
    11041 gaggccgtat tcgataccgt cgaaaccaaa taccaggacc tgctcctcgc cacaatgcgg
    11101 acatgtcagg taaaaacgaa acgtcagatc ggcagcgtct tcgagcattt cgatatgact
    11161 tttgccggta acggtcggcg tcgaaccaaa aatcgccttc gggtacgctg cccctttaat
    11221 tcgaacgagc gccagttcga tgggcgagcc ctcgcctttg ccttttttgg caacttccag
    11281 cggccagccg tcaacttcgt caccctcgac aacctgtttc gttagtcgcc ggaaatttcc
    11341 tggtgcactc gcgccgcgaa aatcaagaat cgcgccgctc atttctttac gctggagatt
    11401 gttacgctcg ttgcttttat cccagtcggg gaaaattttc tgaatcactg gcatttcggc
    11461 gatagcgggg tcaacctcgt cggcgacaaa tccatccgat tcgtcatcga taggctgata
    11521 gaccacggca ctacgttttt tgtgctcagc gaaatagagc agcgccgcga cgagtatttt
    11581 tgtataaccg agacgagctg atttgcgaac agacacgatt tttatcgcgt cgttcgtcat
    11641 catattgagc atcactacct ggaccggctg agtcgtccag tgcccagcga tgtggctgga
    11701 gccctcaggg agataaaaat atttatcagc ccactcaaca cccgtcatcg gtatcgtcac
    11761 gcgcaaggga ttcaaccctg tcgagatcgc gttcgatatc gctgacatcg taatcgctga
    11821 agtcgattcg tatgtctgac aactcattta aggctatcgc cagctcctct cgtaatacac
    11881 tgctcgcctc ttgcggcatc tccggccaga cctttttcag gcgtggtggc cacgactcga
    11941 cgcgggtacg taattcaacg gcgacgcgag atacagcgac gctaattaat tcaattggcg
    12001 cgtagcgttt agcgagaatg cggcgtttta ctcgtgccat caggattcgc tcctgtcgtt
    12061 cctcattttt cagccactgc tcgcgatttt tttcgggaga attgtccccc gcttccggtt
    12121 cgtcattact agttgtgtcg cgtctattgc tccggagata acgaatataa aaatggcgcc
    12181 atgcgtctag ctcccaatca ccacgtcctt gtgggactgg tgctcccgga agttttgcga
    12241 gatcgcgcag acgacgatca gatagcagta aatgtgctgc aacctcagcc tgacttgcca
    12301 ttaaaaatcc tcaccggaac cggaaatacc caaaatgaaa aaatactaaa aacgagcgag
    12361 tttttgcgcg tctaccgacc ctcggtgttt tggatttcgg aaaggacccg cgaccagggg
    12421 gctatctccc tttcaggaca ggaattaggg gctcgcatca ggttcatcaa actcaactgt
    12481 agcctcgcgg cgtacttgtt tgcatacatc agctttgtgt atctgattat cagcgtaagc
    12541 tttcactttg gcagcgagcc atccttttgt gctgtatcga tagactaccg aacaagtttt
    12601 ccatatatgt atatatcttg aacttattga tatcattaca gaaaaacaac aaccatgaga
    12661 acgcaaaaaa tgaacagaga aaaaattgaa gccttaatga gcgtaatatt aaaagaagct
    12721 gacccaaaaa agtttcctga gaagttatca gaggacgcca tcgtatcttt ttatcgtgcg
    12781 aactcctctt atctaaattg ttcaacttta agagaatggc taagcggcga agcagagcct
    12841 tcagatgaga tacttcagaa gattatggtt cagaacaact ggcgcgcaac aaaatggtca
    12901 aatcagttac cttaatctgt tgaatgtgtg ccgccttaaa acaaaaaggc ggcctctcgt
    12961 taaagtaaat taccctattt tccaccaagg catgaagcct ctgccccggt tgaatcaata
    13021 caaccaccgt tcgcagtagt aatcttcgta cccggcgcga tcatcacgtg tttaatactg
    13081 ctgccgtcag cgcgagtcgc ttcggtgtat gtcgaatgtg tgcagcctgc cagcgtgaag
    13141 aacgcgacag ctaagagaag ttttttcacg ttttacctac gcttctgaga tagatttctc
    13201 agcattcacc aatggaagtg attgtggaac atccgtttga cctgctggcc agcggtagcc
    13261 tgttacgcgg gaacgaggaa aagctttgat atttacttca tctgcctggt tgccccccaa
    13321 aatcattaaa tccccggcgg catttttccc gacaacaaag ccgacgtgac caccgccaga
    13381 ccgagacaga atagcaatgc atccataggc gggttccttc agttcattac cccaatcgag
    13441 ataagaattc gcagactcaa aacgcgttga gcgaataccc gcacgttcca gcattgcccc
    13501 ggtgaaagca gcgcaccacg gtgtttcgtc atccttaatg ccgccgcgtt tgatatcttt
    13561 ccagtactgc acaatttctg cagcatgttg cttccctttc atttcccgaa tgccgaggtt
    13621 ttttcgagcc tcgattagcc atttaggatc cgtcattacc tgtctcctgt tatttttgaa
    13681 atattccctc gtgcccgcca gacagcaata cagatggtga ggttgagaaa gaactctgcg
    13741 ggatcaacct gcgtatattt cccgtaaaaa attcgtaggg ctatccacgc agcagacaga
    13801 atcgcgaggt aagcagttag tgagatgaaa aaacgatagc ggcctgtttt ttgaaaaaac
    13861 atcaaccgta gtgccaacat cagacaaata attgcattgg cattgatgag gacagagtgc
    13921 agggtcatta tttcatcccc ttatctgatt tccataaacg agcttgtgac atgatccgca
    13981 acagtgatgt aacgctgatt actgatgcaa ccagtgcacc aatggcggga gagactttta
    14041 ctgttacggg agggttcagg tgactcagca ccgcgttcag aataccggta atgatggctg
    14101 acgctgtctc agcgcaatac acaccgccaa caaacgaaat gagtgcgaaa ataacctgct
    14161 tccagaatcg atgctgcccg gacgacaaca catagagagc tgcgcctgcc agcgcacaga
    14221 tcatcactgc aggtgttgcc tcaggaaata acgcagcaaa cgtcaccccc gccgagccag
    14281 cagcaacgcc agcggttaga ggttcagaca tgatttttcc tgattcagaa acgacaaaac
    14341 cccgccgtgg cgaggttctt agaaatttgg caacatacca aattagcgtc aaatatggcc
    14401 tattttgttc gtttttgcaa gcattatacg caaacgtcgt ctaaatggtt ctgataatga
    14461 tgcaaagttg caagaagtgc cacttcatca agtgctctca ccgcctcttt cattcctacc
    14521 cagtgcaggg agtatgtttc gcaccaggtg gaacgagaaa ccgacagcat tcctgctagg
    14581 gtagatccgg ccatctcctt aaaatcctcg cgtttatttg ctgctgcgac cgcctgcacg
    14641 gacaaccaca ccaacgaaag caggcgctta actacttttc tctgagttga cgcggggatg
    14701 tttcctttat agcgactcca gacagcctca caaatagctg tttggtgttt gaatgccagg
    14761 ttaccaccgt agcaataacg caaccacgct tgctgacttg catccagcga gttcacagaa
    14821 cgccgccagg atgaatcagc aaattcaaaa tcactcatcg gcggcgcggg gcgacgtctg
    14881 ctgaaacttt ccagagcgta aactggcgta accagagcat caacggtccg gtcctccagc
    14941 tgcacacggt gaatatgttt gcgtgggtat gcgtttttgt ctgccggtgg gtgctcacac
    15001 aatgcttcca gttgcccctt agaaccacca gacaaatccc gcaatgcagc gcgcagtttt
    15061 atgcgggtat attccaataa atactcgtta ctcatcagat aaactcctca tcgcgccaga
    15121 tagccagtgt tctgaaaact ccctcagcgt gcatcaacaa caatgagtcg tgctcgtaat
    15181 ccgtttttgt acgtccgtcg atggcatcgt gacacgcact gcaggcgata gcgccctgca
    15241 tatcgtgagg tttgatggct gtgccgcacg ttccggcgag acgataatga gccagtacgc
    15301 tggtttcagg attgtgattg cacacaccgg ggatacgcac agtacaagcg cgactgcgtg
    15361 cagcttttct caggtcgatt tttttcatga tgtgtaatcc agcagctgcg cggcggcatt
    15421 ttcagcggcc tcctgagagg caaatgtgcg gaacaaaata taattccaca gcacatctaa
    15481 aacggctgaa tacagttgac cgaactcaag atcgtccatc gctgcaaacg agattgaacg
    15541 aggtacgcgc atcaaactgc cgtctggcat ctgatgaacg tcataaaacc cagcttccat
    15601 aatcgcccat gcgcgaaacg cctcgaacga cttcaccgca ctgatactcc ccgcgcgctt
    15661 ttccgactca tcgagaagat actgatcagc cagctctgcc atgacatcac cgtgtccacc
    15721 gtaatacgcc agtaacttaa cgtagccgtg aaccagttcg cgctccgcag gagaaatagc
    15781 ccctccggac ggatgccagt aatcaaagcc caggttgaga agtgagaaaa atttacgatg
    15841 aaacgcggga ttacgcgcct gtttgaaatc tgcatacaca acagcacctg gtttgactcg
    15901 ttcagtcaac cagcgttgcg tgtctggtgt cgcgggtatt agcgaaccgc cggataccct
    15961 gataaatgaa tgctgcgcca tggttaactc caggtggcgc ggcagtgttc agaaaatggt
    16021 gccgggtgtt caatccagca ccgatattat accgtgtttt acgcgtcagg gggtgtaata
    16081 gtatatccag ctatttctgc caactcgaga aacgccatca ggctggcaat ccgctcatca
    16141 tcggtcagaa cacgcacacc aacgatgtgt ccgtccttac gcttgataac agcccggcat
    16201 gtttccggta gttcgcctat agattcttga atattcatgt cttcccccca atgccacaac
    16261 ctgtatattt atacagtatt ttatggcatt tgtcataacg ccaaatacaa tgagggaata
    16321 atgaacaatt atattcacgt cgtgaatttt attaaacggt tttatagggg attaaaccgt
    16381 ttatggtcat tatttatcac tcctgtaaat tggaattaac cattttctaa tcgtcgacgc
    16441 tgcaggtggg tcagagtatt tcgccacaaa aataccggat atgtattgca cgccacgcga
    16501 taatggcgct gtcgggtttt cagtaaaaaa acgatcagcc agttcaatgg cttcgctttt
    16561 taatctgtgc tgtggtcgtc ctccgccctt tgttcccagg tatcgcgcca gttgtttgaa
    16621 acggtaggcg atgattaacg gtcgcatcat catattgaaa aaatcgcggg catcgttgtc
    16681 agcgccttgc gctatcaaca gtgcaaaaaa cagatacggg attatgtaca cttcaggaga
    16741 tatacgaacc cgttcacaag ttggtggagc caactcaata agtaagtcag ccagcggatt
    16801 ccccttttct ccacgtttgt aatagcgata cagcggcccg atatcaagtt cactcacgtc
    16861 gatttcagga tgcccggcaa cgactgtacg tatctcttct gccagagcgt caaatattgc
    16921 cccggtccgt tcaagataga gcctccgggt cgaaccgcga tcatccaacg cccgtaacat
    16981 atctaaccgg ctaacgaaag tttcaggcca gtcctcatag aaggttttca taattacctc
    17041 gaataataaa aactgcacta tttgcacaat ttattattcg ttttcacgcc gctgagttct
    17101 ataaaaaaga aaaaacctcc gaggcagggt tttgttatgg ctcgttaaat catcatccaa
    17161 ctttttagac tgaacggtga tacttgcaaa tatttgaaaa tatgggctgg aaccctatct
    17221 tatgatgtgc ctggtaattt atattgttgg gtctttatat catgaggaat cactatgggt
    17281 acgttaggca accagccgtg cagaagtcgt tatgagttcg atgattttaa tttagccaat
    17341 cgagctgcgg ctcttactaa gttagcaaaa gaaactggtg tatcggtgga taccttaatc
    17401 aaacttgaaa ttgctatgca actgaatcgg ctcaactcca tccttatcga aaacggtgac
    17461 aagatggacg agaatctggc aggtattggc gagatactgc agaagtgaga gtagcccctc
    17521 ttctgagggg ttgcacattt aaaatattaa gggcggtctt cttcacgtac acagtcacct
    17581 ggtcgaatcg ttcacgcata ctgagtacaa aaaatctcaa cgataagatc aggttagacc
    17641 tgtattttcc agaaacatat agacgcctaa aaaaacacta aactcatgat tatcatgtat
    17701 gtttttactg gaaacattga accatccaca atacagcgta aaaagcaagc aattcacaag
    17761 attttaaagt gatttatctg gaagacttta ctactaacag ttaatatcgt accagaagtg
    17821 cagaatttgc acttttacag gaatgtggta cggaacatgc tgtctatatt tcaaccaata
    17881 atcgtaatgc agtaactgga ggagctatgg ctaccaaccc aagagagcac gcccggcgag
    17941 tgctggacac gttctggtgc gggcgtggtt tccctgttga tccagcgaaa attgctcacg
    18001 acatgggatt agatgttttt attaccgatc ttcctgggaa agtttctggc gcactgatta
    18061 aacaaaaaga tcaagatcca gcgatctttc taaacagtga tgacaacaaa gttcgtcaac
    18121 gtttttcatg tgggcatgag ttggggcatt acatcgcccg gcaagcatcg catagcgatg
    18181 aatatgagta cgttgattta cgtggcgaaa ccgcaagcgc cggaacgcat cctgatgaaa
    18241 tttttgcaaa caagttcgca gcagaacttc tgatgccggt agatgaggtc agaaaactgc
    18301 acgctgacgg tcaaccaagt tatataatgg cgcattattt tggcgtatct gacgatgcga
    18361 tgacttatcg tttaaagaac ttgaggttgg ggtaacgctt taaaatgcca tcggatgacg
    18421 attttaacag ccgtttcaaa caggaagtag cacgcagact gaaggattcc ctcaacctac
    18481 accgtcagtt agcagaagag gttgagccgg aggacgatga aactccggtt cagtctcctg
    18541 acgaagcttc acctcagcca aaggtttcgc acgcccccaa aaaaacggct gtccgtattc
    18601 gcggaatagc cagaaaaaga gctgccgaag ctcgcaaaat ggctgtagct gcgtctagca
    18661 aaagaattcc aactgctaca ccctctcaac aagtcacccg tactgagagt aaaatcttga
    18721 ccgagttcac atctgtgaaa caggtgaatt cacttgatac cgagcatgag cgcaaacaac
    18781 tcaacaacaa agattttgaa gcagaaatcg ctctaaaaaa acgatacggg aaatggttcc
    18841 tgattatcct tgctttccaa cttctgataa tgaatggtat atttattgcc gacggcgcag
    18901 gtaagctgga atttgaggat ttaacactac agttgtacat gggcggtact ttgacagagg
    18961 tattcggcct cgtgctggtc gtgacaaaat acctttttaa acgcaagtaa ctctctcggt
    19021 ccaacagtct ttcgaaggcc ctgatcaaag ggccttgata aattagcagt gcgttgaaaa
    19081 cagatagtta ttcccatcag ttccaacgta atacacatca aaaaaatcac cattcttttg
    19141 ctctaacgtc aacacctacc cttaaaaaaa taattctcca agaatgagaa agttattgtg
    19201 tgtattagat aagatatgag ctgacacatc tatggcgtgg agccaaattt aatatgacag
    19261 tccgctctgt gccataagcg gactgtcaat aaacagattc acttgtaaat cttgaagcta
    19321 tacatattta ctttcaatga taaataaaag aactatccaa aaatcactga gagcatattt
    19381 taactaaccc tccgctacta ttaacataaa tttcacccaa ccataattaa attgatacat
    19441 atagcaactt accttgatac gaatcatctg ggttatattt ttccatattt ttttccgtca
    19501 aatcgaatca tgccaggatc aagaaaatta attaaatcga ttaacgcgtg ctgtattctg
    19561 attagccttt ctttat
//
LOCUS       DAJOTB010000088        18982 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJOTB010000088 DAJOTB010000000
VERSION     DAJOTB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.088"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            63..719
                     /locus_tag="OHN28_003068"
     CDS             63..719
                     /locus_tag="OHN28_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCQ2298418.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            743..1405
                     /gene="exoX"
                     /locus_tag="OHN28_003069"
     CDS             743..1405
                     /gene="exoX"
                     /locus_tag="OHN28_003069"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCQ2298419.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1402..3462)
                     /gene="ptrB"
                     /locus_tag="OHN28_003070"
     CDS             complement(1402..3462)
                     /gene="ptrB"
                     /locus_tag="OHN28_003070"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCQ2298420.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RRLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(3671..4330)
                     /locus_tag="OHN28_003071"
     CDS             complement(3671..4330)
                     /locus_tag="OHN28_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCQ2298421.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(4657..5013)
                     /locus_tag="OHN28_003072"
     CDS             complement(4657..5013)
                     /locus_tag="OHN28_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCQ2298422.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(5080..5370)
                     /gene="yebG"
                     /locus_tag="OHN28_003073"
     CDS             complement(5080..5370)
                     /gene="yebG"
                     /locus_tag="OHN28_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCQ2298423.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            5504..6682
                     /gene="purT"
                     /locus_tag="OHN28_003074"
     CDS             5504..6682
                     /gene="purT"
                     /locus_tag="OHN28_003074"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCQ2298424.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(6738..7379)
                     /gene="kdgA"
                     /locus_tag="OHN28_003075"
                     /gene_synonym="eda"
     CDS             complement(6738..7379)
                     /gene="kdgA"
                     /locus_tag="OHN28_003075"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCQ2298425.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(7416..9227)
                     /gene="edd"
                     /locus_tag="OHN28_003076"
     CDS             complement(7416..9227)
                     /gene="edd"
                     /locus_tag="OHN28_003076"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCQ2298426.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(9462..10937)
                     /gene="zwf"
                     /locus_tag="OHN28_003077"
     CDS             complement(9462..10937)
                     /gene="zwf"
                     /locus_tag="OHN28_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCQ2298427.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11275..12144
                     /gene="hexR"
                     /locus_tag="OHN28_003078"
                     /gene_synonym="yebK"
     CDS             11275..12144
                     /gene="hexR"
                     /locus_tag="OHN28_003078"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCQ2298428.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            12272..13714
                     /gene="pyk"
                     /locus_tag="OHN28_003079"
     CDS             12272..13714
                     /gene="pyk"
                     /locus_tag="OHN28_003079"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCQ2298429.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13846..14817)
                     /gene="lpxM"
                     /locus_tag="OHN28_003080"
                     /gene_synonym="msbB"
     CDS             complement(13846..14817)
                     /gene="lpxM"
                     /locus_tag="OHN28_003080"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCQ2298430.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(14936..16258)
                     /gene="mepM"
                     /locus_tag="OHN28_003081"
     CDS             complement(14936..16258)
                     /gene="mepM"
                     /locus_tag="OHN28_003081"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCQ2298431.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(16274..17206)
                     /gene="znuA"
                     /locus_tag="OHN28_003082"
     CDS             complement(16274..17206)
                     /gene="znuA"
                     /locus_tag="OHN28_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCQ2298432.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            17285..18040
                     /gene="znuC"
                     /locus_tag="OHN28_003083"
     CDS             17285..18040
                     /gene="znuC"
                     /locus_tag="OHN28_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCQ2298433.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            18037..18822
                     /gene="znuB"
                     /locus_tag="OHN28_003084"
     CDS             18037..18822
                     /gene="znuB"
                     /locus_tag="OHN28_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCQ2298434.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
BASE COUNT         4512 a         4892 c         4725 g         4853 t
ORIGIN      
        1 aaattatgag tattttctaa accgccccca taatttgcag tcattttgaa aaggaagtca
       61 ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc tcgtaaaacc tcactcaccg
      121 aacaggtagc tcatcatctg gagtttgtga gggccgccgc caggcaacaa tgtcagctgt
      181 tagtttttcc atcgctttct ttactggggt gtgattattc gcgacgtgcc cttcctgccc
      241 cacccgatct ctcgctatta gacccgcttt gttatgccgc aacgacctgg cgaatgacca
      301 tcattgccgg ccttcccgtt gaatataacg atcgctttat tcgtggaatt gcggtattcg
      361 caccgtggcg aaaaacgcca gggatctacc atcagagtta tggtgcatgt ctgggccgtc
      421 gttccagaac catcacggtg gtcgatgaac aaccgcaagg tatggatatg gacccgacct
      481 gttcactgtt tacgacaggg caatgtctgg gagaacccga cctgctggcg tcggcccgtc
      541 gcttacaatt tttttcacat cagtactcga ttgctgtgct gatggcaaat gcccgtggta
      601 acagtgcact atgggatgaa catggtcgtc tcatcgttcg cgccgatcgt ggttcattat
      661 tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga tatcattcca ttacgctagg
      721 ctttttcggc ctggagcatg ccatgttgcg cattatcgat acagaaacct gcggtttgca
      781 gggagggatc gttgagattg cctctgttga tgtcattgac ggaaaaatcg tcaatcccat
      841 gagccacctg gtgcgtcccg atcgtcctat tagtccacag gcgatggcga ttcatcgcat
      901 caccgaagcc atggtcgccg ataaaccgtg gattgaagat gtgatcccgc actattacgg
      961 tagtgaatgg tatgtcgcgc ataacgccag ctttgaccgc cgcgtactgc ctgaaatgcc
     1021 cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg tggccaggga tcaagtacag
     1081 caatatggcg ttatataaaa cacggaagct caatgtacag acgccgccgg gcctgcatca
     1141 tcaccgcgcg ttgtatgact gttatatcac cgctgcgttg cttatcgata ttatgaacac
     1201 ctccggctgg acggcagaac agatggccga tatcaccgga cgtccatcgt tgatgacgac
     1261 tttcaccttt ggcaaatacc gtggcaaagc ggtttccgac gttgccgaac gcgatccggg
     1321 ctatctgcgc tggttattta ataacctgga cagcatgagc ccggagctgc gtttaacact
     1381 gaaacattat ctggaaaata cttaatccgc aggcgtagcg ggtaatgttc cctgcgccag
     1441 cgcgaccaga aaagcatatt ccatcgctac gccttcgtac gatttaaagc gaccagattt
     1501 accgccatgg cctgagtcca tgtcggtaca gagcaataaa agatggttat cggttttcag
     1561 ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat tgcacctgag aatcgtgtaa
     1621 accggtcgtt accagtaaat gcggataagc ctgtgcggtg acgttgtcat acgggctgta
     1681 gcttttcata tactcgtaat attgcggatc ctgcggattc ccccactctt caaattcgcc
     1741 ggtggtaaga ggaattgatt catccagcat tgttgttaca acatcaacaa acggcacctg
     1801 ggcgataacg ccgtggaaca attccgggcg ttgattaatc gcaacgccca tcaacatacc
     1861 ccccgcactc ccgcccatcg cataacagag cgaaggagag ccatagccca gttttaacaa
     1921 tgcatcgcag gcatcaagat aatcattaaa cgtatttttc ttcttcagaa atttaccgtc
     1981 ctcgtaccat tgttgcccca gctcaccgcc gccacgaaca tggacaatgg cgtagacaaa
     2041 tccacgatcc aacaaactca agcggctaaa actgaaatcg gcatcaatac ttgcgccgta
     2101 agaaccgtag ccatacacca gcagcgggtt gtgtcctttg cgaaaatgtt tgcgatgata
     2161 gaccagcgaa accggaactt cgacgccatc acgggcgact atccacaggt gttcactgcg
     2221 gtaattcgcc gcatcaaaac caggaacttc cgtttgtttt aatacacgac gctcaccggt
     2281 atccatatcc agttcaaaca aagtatctgg tgtggtcatg gacgaataac cataacgcaa
     2341 tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc acataggccg gatcatcaaa
     2401 ggcaatgcca atgacttccc gggtcttgcg gttaatttgc cgcaaactgg ttaacctgcg
     2461 ctgacgctct tcaaccacca gccagtcggt aaacagcgta aacccttcca gcataatgtt
     2521 atcacgtggc ggaattaact cttcccactg ttgctcatca cgcatacggg tacggtataa
     2581 gccaaagttt ttaccgtggc ggttggaacg cagataaaac cgatgctggt agtgatcaag
     2641 gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac ggctcggcat cggctatttc
     2701 cgcgtccagc aggcgaactt cactggtggt ggcgctggcc agatgaatga ctacatagtg
     2761 cttcgaggtc gttttatgca ggctgacgta ataggtatcg tctttttctt cgtagatcag
     2821 tttatcctgc gatgctggcg taccgatggc gtgacgccag acctgataag gaagcagcgt
     2881 caccggatgc ttgcgaacat agtagaaagt ccaggaatca tttgcccaga caaagctggg
     2941 gtcaacgtta tccagcagtt ccggatacca gttacctgtt tccagattac gaaaacgaat
     3001 gccgtactgg cgtcgggaaa gaaaatcttc tgccagtgcc ataatggtgt tatcgggcgt
     3061 aatcgccatt ccgcccatcg aataaaactc actatgagcc gcgcgcttgt tggcatcgag
     3121 caatgtttcc cactcatccc actcttcgct gaacgccgat tgacgctggt agatagcata
     3181 ttcacagcct ggttcataaa tatgccgata gcggtagcca ttttttatgt agggcgcaga
     3241 aacttctctt tgcggaatgc ggtcgatgat ttcctttaag atgcgatcct gcaaggcttg
     3301 ttgcgaggcc atcacccgat gaccgtaact attttcttgt tgcagatagt ccaggacttc
     3361 cggctgagaa cgcgtatcgt cccgcagcca gtagtaatta tcgatgcgcg tatcgccatg
     3421 aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc atgttattgt tctttctggt
     3481 tgaaacatct tataagggtg gcaaaactca ccggggatgc aagcgaaaca gggtagtcat
     3541 tgcttagatg atgacaggta atggcgcgga tatcgactgt tatgcagaca gagaaaatca
     3601 gcctgttcaa atggctgtgc ggttctggat aacccgaaat agtcaacttc aggctatcca
     3661 gagagcggaa ttactccgcc aaagtacgtt tttgctgttc gagatcgcgt tcaatgcctt
     3721 cacgaacgtc ctgggggatt ttcagcgcat cacccagtgc attcaggtaa ctgcgttcca
     3781 taaaatggtc aatatcaata gccgcgcagc tcaggaaata gatttccagc gcctcttctt
     3841 cattgcggac cccggtagcc aggcgttgtg gatccagcgg ttgttcgatt gcctgctcaa
     3901 tgagtacacg ccccttctct tccacaccgg cttcacgcaa ttgcaggtcg attgccgcac
     3961 gttctttggc atcaatatga ccatcacttt tagcggcaaa gaccagcgca aggatcaaac
     4021 gttccgtacg ctcatccagc ggcgtacttt gcgcgccaaa ctgcggttcg tcctgatgcg
     4081 ccgcgcgaat tttatctttg tatttattcc acagcaccgt acccgctacc gctccgccgc
     4141 caaccagtaa cgcgtttgtg ccatatttcg taagaagttt acgtgctgat ttatttgcga
     4201 ccagcagccc agccagtccg cctaatgcgc caggcactaa aagtttgccc aatccctgat
     4261 ccgcagacga ggaggtagaa gaacggcttt gcccaagcag ggattgcagt tgatttaacc
     4321 agttagccat atttgctcct caataaccat ttttagtgcc tgtagcatag cggatgagga
     4381 tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa gcggggcggt aggccgttat
     4441 tcaaagaaat tcgcgccagg taaggtgttc actgtatata aggtgataag ggtattttag
     4501 gtgaaagtag tttgacattt tgagtggaac tgtttgatgc tgattgcccg gttcgacgct
     4561 actgcgtctt ctcaggccta cgattgtgtt gcgttgtagg gcggacaagg cacccgcgcc
     4621 gcatccgcca acggtcaatc gaattatcag aagccattaa cgccgctgat attccgccat
     4681 tcccgctttg cagtccacgc tgacctggta atgaatatcg gcacttttac cacgcacggt
     4741 tagcggtact gaccatttat catctttccc ctgaatgtcc tgcaaactga cccatgccac
     4801 gggatcggcc tgaccgacaa ttttttgatc atcagcccag cgcgccacgc gattttgttg
     4861 gtaatcacgt tttacgctcg cggcaattcc ggcagcatcc agaccttcac actttgggaa
     4921 agtgaccgac ttgctggttt cattattggc agcgaaaact gatgcgcagg cagaaaccaa
     4981 caacagccct aaaaacgccc ctcttttttt catgtttttc tccatagcac aatgattcag
     5041 gagaaagcat ggtacaaatt gtcaggagcg caagttgctt caggccgcgt gtgaggcatc
     5101 ttcctcttcg gattcagcac cgacgacctg tggggacggt aatttgccgg ttttcagaat
     5161 ggtgctcagc acatcttttt gttctgccag ccatagcgaa agggcttcac gttgctcgtc
     5221 ttccatttgc actggagaat ttgtcagcca ggtgtcaagc agatcggccg tatcaagcat
     5281 cttgtcataa gcatcggctt cctttttgct ggtaaacgac attttctctt cgccctcacg
     5341 aatgactacg tatttaactt caaccgccat ttgcagcctc tcataataac tgtggtttta
     5401 tacagtatat ttcttttcag ttgggaaatc aacatcagca ataaagacac acgcaaacgt
     5461 tttcgtttat actgcgcgcg gaattaatca ggggatattc gttatgacgt tattaggcac
     5521 tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc tccggtgaac tgggtaaaga
     5581 agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt gccgtcgatc gctatgccga
     5641 cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt aatatgcttg atggtgatgc
     5701 attacgccgt gtggttgaac tggaaaaacc acattatatc gtgccggaga tcgaagctat
     5761 tgccaccgat atgctgatcc aacttgaaga ggaaggactg aatgttgtcc cctgcgctcg
     5821 cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc ctggcggcag aagagctgca
     5881 gctgcccact tccacttatc gttttgccga tagcgaaaac cttttccgcg aagcggttgc
     5941 cgccattggc tacccctgta ttgttaaacc ggtgatgagt tcttccggta aagggcagac
     6001 gtttattcgt tctgcagagc aacttgctca tgcatgggag tacgctcagc aaggtggtcg
     6061 cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag tttgacttcg aaattaccct
     6121 gctaaccgtc agtgcggtgg atggcgtcca tttctgtgca ccggtaggtc atcgccagga
     6181 agatggcgac tatcgtgaat cctggcaacc acagcaaatg agcccgcttg cccttgaacg
     6241 tgcgcaggag attgcccgta aagtggtgct ggcacttggc ggttatgggc tgtttggtgt
     6301 cgagctgttt gtctgtggtg atgaggtgat tttcagtgaa gtctcccctc gcccgcatga
     6361 caccgggatg gtgacgctaa tttctcagga tctctcggag tttgccctgc atgtacgtgc
     6421 cttcctcgga cttccggttg gcgggatccg ccagtatggt cctgctgctt ctgccgttat
     6481 cctgccgcaa ctgaccagtc agaatgtcac gtttgataat gtgcagaatg ccgtaggcgc
     6541 agatttgcag attcgtttat ttggtaagcc ggaaattgat ggcagccgtc gtctgggggt
     6601 agcactggct actgcagaga gtgttgttga cgccattgaa cgcgcgaagc acgccgccgg
     6661 acaggtaaaa gtacagggtt aaacccggca aaaaaacgct acaaaaatgc ccgatcctcg
     6721 atcgggcatt ttgactttta cagcttagcg ccttctacag cttcacgcgc cagcttagta
     6781 atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa ccagccagga accaccgatg
     6841 cacagcacgc ttttcagcgc caggtagtca cggtagttag ccggagaaat accacctgtc
     6901 gggcagaaac ggacctggga gaacggaccc gcgatcgcct gcagggcttt cacaccgccg
     6961 ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt agtccatacc cagcatcagt
     7021 tcggaaacag tgctgatccc cggaatcaga ggaatagtcc cttcggtagc agctttcagc
     7081 agcggctcgg tcagacccgg gctaattgca aactgtgcac ctgcttcagt gacttctgcc
     7141 agctgctgtg gattcagcac cgtaccggca cccacaatcg cttcaggcac ttctttggcg
     7201 atagcacgga tagcgtcaac tgcgcactcg gtacgcagag tcacttccag aacgcgcacc
     7261 ccaccagcaa ccaacgcttt tgctatcggc accgcgtgtt ccagtttttt taccacgata
     7321 accggtacaa ccgggccggt ggtcaggatt gattctgcac ttgttttcca gtttttcatc
     7381 agagttttct ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca ggttgcgccc
     7441 tgttcggcac cggacagttt ttcacgcaag gcgctgaata attcacgtcc tgttcccacg
     7501 cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag ccagttccgc ttcgtctacc
     7561 agcagcgtca gttcgcctgt ctgtccattc acacgaatga tgtccccgtc gcgcactttt
     7621 gccagcagcc cgccatcgta ggcctctggt gttacgtgga tagctgacgg cactttacct
     7681 gaagcgccgg agagtcgtcc atcggtaact aacgcaattt tgaaacaccg gtccaataat
     7741 acaccaagtg gcggcatgag tttatgtaat tctggcattc cgttcgcttt tggcccctga
     7801 tgacggacaa caacgacaca atcgcggtcc agtaaacccg cttcaaaggc cggcataacg
     7861 tcatgctggc tttcaaaaac aaccgctggc gcttcaatca cctggttctc taccggcacg
     7921 gcagaggttt tcataaccgc tcgccccagg ttaccgctta acacttttgt cccaccatga
     7981 tgagagaaag gttgttcgaa ggacgcgatc acattgttgt cgagtgattt ttctgcccct
     8041 tcccgccagt ccagttcacc attattcagc catggttcaa gggtataacg agacagacca
     8101 aagcctgcca ccgtattgac atcttcatgc agcaggcctg ctttgagcag ttcacgtacc
     8161 agaaccggta cgccacctgc cgcctggaag tggttaatat cggccggacc gttcgggtag
     8221 agacgtgcca tcagcggtac aacatcagaa aggtcagaga agtcatccca gttaatctga
     8281 ataccggctg cgcgcgccat cgccactagg tgcatggtgt ggttagtgga accaccggtc
     8341 gccagcagtg caacgatacc gttcaccacc actttctcat cgatcatctt accgatcggc
     8401 atccattcat taccattacc ggtcatgcgc gtaacctggc gcgcagctgc agcggtcaaa
     8461 gcatcgcgca gcggagaatc cggatgaaca aaagaagagc ctggcaactg catccccata
     8521 aactccacca ccatctggtt ggtgttggca gtaccgtaga aagtacatgt tcccggcgca
     8581 tgataagacg cggcttctga ctccagtaat gccatgcggt ccactttacc ttcggcataa
     8641 agctggcgaa tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg tccagacggc
     8701 acaaacaccg caggcaaatg accaaacgac agggctgcca tggtcagacc tgggacaatc
     8761 ttgtcgcaca caccgagaaa cagagcacca tcaaacatgt tatgggacag ccccaccgcc
     8821 gcagacatcg caatcacttc gcggcttaac agcgacaatt ccattccatc ctgcccctgg
     8881 gtgacaccat cacacatcgc cggaacaccg cccgcaacct gaccaaccgc attcgcttca
     8941 tgcagggctt tacgaatgat ttctggatag tgttcataag gctggtgagc ggagagcatg
     9001 tcgttatagg aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa agaggctttg
     9061 tcttctggct ggcaggcagc gaaaccgtgt gccaggttac cgcatgccaa ctgcgaacga
     9121 tgaacggtcg aagttttcgc ttgttctatc cgggcgaggt aagcagagcg agtctcgcgc
     9181 gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg ggttcataaa ggctcctgaa
     9241 attgagttgt cagagcagga tgattcacaa cgcgtttcat acatggaaat tatgattgaa
     9301 acgcctgtaa ccggagctca tagggcaaaa acgtttcagt cagtgtaata aaaaaagcct
     9361 cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc tgtgcaagat
     9421 catgttaccg gtaaaataac cataaaggat gagcgcagat attactcaaa ctcattccag
     9481 gaacgaccat cacgggtaat catcgccact gaggcaacgg gtccccaggt tccggcctga
     9541 tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag taatggagtc tacccatttc
     9601 caggcttctt ccacttcgtc gcgacgtaca aacagtgcct gaataccacg catggtttcc
     9661 agcagcaaac gttcataggc atccgccaga tgcgtctgat taaaggtttc tgaatagctc
     9721 agatccagct tggtgatttg caggttatgt ttgtggtcaa ggccaggaac tttattcagt
     9781 acctggatat ccacgccttc atcaggttgc agacggatag tcagtttatt ttgcggtaga
     9841 tcctgccacg actctttaaa cagattcagt tcaggtgttt tgaaatagac cacgacttca
     9901 gaacatttgg tcggcagacg tttaccagta cgcaggtaga atggcacacc ggcccagcgc
     9961 cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg tattgctgct cttgttcgcg
    10021 ccctcttctt ccagatagcc cggcactttt ttgccctgag cgaagcccgc agtatattgc
    10081 ccgcgtacgg ttttttcgcg cacattggag cggtcgatgc ggcgcagaga cttcagtact
    10141 ttcacttttt catcgcggat gctgtctgcg ctcaggtcag acggcggaga catcgcaatc
    10201 atgcaaagaa tttgcagcag gtggttctgg atcatgtcgc gcatctgacc ggctttatca
    10261 aaatagcccc agcgcccttc gatccccact tcttctgcca cggtaatctc aacatgatca
    10321 atggtgcgat tgtcccagtt gttcacaaac agagagttag caaaacgcag cgccaacagg
    10381 ttcagcactg tttctttacc aagatagtgg tcgatacggt aaacctggca ctcctcgaag
    10441 tattcgccaa cctgatcgtt gatttcctgc gaggtcgcca gcgacgtccc cagcggtttt
    10501 tccatgacta cgcgtgccgg tttagcattc agttttgcct caccaagccc tttgcaaatt
    10561 gcgccaaaag tgctgggcgg catggcaaag tagttaatgg tgatacgatt tttttgatcc
    10621 agcatcgcgc cgagacggct gaatgcagca gtgtcattga catcaagatt acaaaaatcc
    10681 agacgtgcac tcagggtgtc ccataaacct tcatcaatgg tttctttcat gaaagtttcg
    10741 agcgcctcgc ggacaacttt ggtataagcc gctttatccc agtcagcacg ccctacgccg
    10801 ataatccggg tgtccgggtt gagctgaccg gctttttcca gttgatacag ggaaggcagc
    10861 aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga ccaggtcaca ggcctgggct
    10921 gtttgcgtta ccgccatgtc attctcctta agttaactaa cccggtactt aagccagggt
    10981 atacttgtaa ttttcttacg gtgcactgta ctgcttttac gagcttgcga aaactgtaaa
    11041 cgcttatcca cccgtgcgat tacggtaaaa gcgcgcaaag tgcggcaaaa ctgataaaaa
    11101 aatcatcgtt ttccattggg taaaaatctg acactgatca tgttatgaaa aaaaataaca
    11161 acttttttat ctgcttttgt cattaacaca gcacacaggc gtaatatctg acaaaactgc
    11221 atttcgattt ctttcagagc ggaaatcgtc attacccgtg agtctcttta catcatgaat
    11281 atgctggaaa aaatccagtc tcagctggaa catttgagca aatctgagcg caaagttgcc
    11341 gaggtcattc tggcttcgcc cgataacgcg atccattcga gtattgctgc tctggcgctg
    11401 gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga cacacgcggt
    11461 tttcctgatt ttaaacttca tctggcacaa agtctggcga atggcactcc ctatgttaat
    11521 cgcaacgtca atgaagatga cagcgttgaa tcatacacgg ggaaaatatt tgagtccgca
    11581 atggcaacgc tcgatcatgt ccgtcattca ctggataaat ctgccatcaa ccgcgccgtc
    11641 gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat taggttcttc agccgccgtt
    11701 gcccacgatg cgatgaataa gttctttcgt tttaatgttc cggtggtgta ctccgatgat
    11761 atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt ggtgctgatt
    11821 tctcacacgg gaagaacaaa aaatctggtc gagctggcgc agctggcacg cgaaaacgac
    11881 gctatggtaa ttgccctcac ctctgcgggt accccactcg cccgggaagc aacgctggca
    11941 attaccctcg acgtaccgga agatactgac atttatatgc ccatggtttc tcgacttgca
    12001 cagctgaccg tgatagatgt gctggcgaca ggatttactt tgcggcgcgg tacaaaattc
    12061 agagataact tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga taagcagtta
    12121 cttaatttaa gtgacgatcg ctaaaaacga ctgtcactgt cctaatctta tacgacatcc
    12181 gaatgagatt aatttatcgc catcacggcg ttatttcatt cggatttcat gttcaagcaa
    12241 cacctggttg tttcagtcaa cggagtatta catgtccaga aggcttcgca gaactaaaat
    12301 cgttaccacg ttaggcccgg caacagatcg cgataataac cttgaaaaag ttatcgcggc
    12361 gggtgccaac gttgtacgta tgaatttttc tcacggctcg cctgaagatc acaaaatgcg
    12421 cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt catgtggcta ttctgggtga
    12481 cctccagggg cccaaaatcc gtgtatccac ctttaaagaa ggcaaagttt tcctcaatat
    12541 tggggataaa ttcctgctcg acgccaacct gggtaaaggt gaaggcgaca aagaaaaagt
    12601 cggtatcgac tacaaaggcc tgcctgctga cgttgtgcct ggtgacatcc tgctgctgga
    12661 cgatggtcgc gtccagttaa aagtactgga agtccagggc atgaaagtgt tcaccgaagt
    12721 gaccgtcggt ggtcccctct ccaacaataa aggtatcaac aaacttggcg gcggtttgtc
    12781 ggctgaagcg ctgaccgaaa aagacaaagc agacattaag actgcggcgt tgattggcgt
    12841 agattacctg gctgtctcct tcccacgctg cggcgaagat ctgaactatg cccgtcgtct
    12901 ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag gttgaacgtg cggaagccgt
    12961 ttgcagccag gaggcaatgg atgacatcat cctcgcctct gacgtggtaa tggttgctcg
    13021 cggcgacctc ggtgtggaaa ttggcgatcc ggaactggtc ggcattcaga aagcgttgat
    13081 ccgtcgtgca cgtcagctaa accgcgcggt aatcacggcg acccagatga tggaatcaat
    13141 gattactaac ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg ccgttctgga
    13201 tggtactgac gctgtgatgc tgtcggcaga aactgctgct ggtcaatacc cgtcagaaac
    13261 tgttgcagcc atggcgcgcg tttgcctggg ggcggaaaaa atcccgagca tcaacgtttc
    13321 taaacaccgt ctggacgttc agttcgacaa tgtggaagaa gccattgcca tgtcagcaat
    13381 gtacgcggct aaccacctga aaggcgttac ggcgatcatc accatgaccg aatcgggtcg
    13441 taccgcgctg atgacctccc gtatcagctc tggtctgcca attttcgcca tgtcacgaca
    13501 tgaacgtacg ctgaacctga ctgctctcta tcgcggcgtt acgccggtgc actttgatag
    13561 cgctaatgac ggcgtagcag ctgccagcga agcggttaat ctgctgcgtg ataaaggtta
    13621 cttgatgtct ggtgacctgg tgattgtcac ccagggcgac gtgatgagta ccgtgggttc
    13681 tactaatacc acgcgtattt taacggtaga gtaagtacgt tgccggatgc ggcgaaaacg
    13741 cctcatccgg cctacagttc aatgataatt caacagattt cgaatcttct gaagcaaact
    13801 tgaacttatc atcagtcgaa ggcctctcct cgcgagaggc attttttatt tgatgggata
    13861 aagatctttg cgcttatacg gttggatttc gcccggtttg cgagttttca gcaattttaa
    13921 tatccaggtg tattgttctg gtcgcggacc aacaaaaatc tcgacttctt cattcatccg
    13981 ccgcgcaatc gtatgatcat ccgcctctaa cagatcatcc atcggtgggc gcacctgaat
    14041 cgtcagacga tgcgtcttgc catcataaat cggaaacagc ggtacaacgc gcgcacggca
    14101 cactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa agaaatcaac
    14161 aaattcgctg tgttctgggc catgatcctg atcgggtaaa taatatcccc agtaaccctg
    14221 acgtaccgac tggatgaatg gtttaatacc atcatttctc gcatgcagac gaccaccaaa
    14281 gcgacggcgc accgtgttcc agacataatc aaaaaccggg ttgccctgat tatggaacat
    14341 cgctgccatt ttctgccctt gcgaggccat cagcatggca ggaatatcga cggcccaacc
    14401 gtgcggcacc agaaaaatca ctttctcgtt attacgccgc atctcttcga tgatctccag
    14461 cccttgccag tcaacgcgcg gctgaatttt ctccggcccg cgtattgcca actcagccat
    14521 cattaccatc gcttgcggcg cggtggcaaa catctcatct acaatcgctt cgcgttcagc
    14581 ttcactacgt tctggaaagc agagcgacag attgattaac gcacgacggc gtgagctttt
    14641 tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg ggatcacgga actttggcgg
    14701 cgttaaagcg atacccgcca tcgctgctac gcccagccat gctccccagt agcgcgggtg
    14761 gcgaaaggat ttatcaaact caggaatgta ttcgctatta ttttttttcg tttccatgct
    14821 tttccagttt cggataaggc aaaaatcaat ctggtgatag tgtagcggcg caacttgccc
    14881 cgcaccaaat aaaaaagccg gtactgactg cgtaccggct gcgaatggat gttaattaat
    14941 caaaccgtag ctgcggcaca atctctttgg cctgtgccag gaattcgcga cgatcggagc
    15001 cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg gtttacggcc tgctggttta
    15061 tccatacttc atagtgcaga tgcggcccgg ttgaacgtcc ggtattaccg gaaagcgcga
    15121 tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag gatcttgcgc aagtgcatat
    15181 aacgcgtggt gtagctgcga ccatgacgaa tagccacata ataacctgct gcgccactac
    15241 gtttggcaac caccacttca ccgtcaccca ctgaaagcac tggcgtacct tgtggcatgg
    15301 caaaatcaac acctctgtgt ggcgcaacgc gaccggtcac cggattagta cgacgcgggt
    15361 taaagttaga tgagatacgg aactgtttcg ccgtcgggaa tcgcaagaat cctttcgcca
    15421 gaccagtacc gttacgatca tagaatttgc catcttcagc gcggattgca taataatctt
    15481 taccttctga acgcaaacgt acgcccagca gctggctttg ctcacgttta ccatcaagca
    15541 tttctcgtga cattaacacc gcaaattcat cgcctttttt cagtttgcgg aaatccattt
    15601 gccactgcat ggctttaatc actgcgctca cttcggcgct ggttaaaccg gcgtttctgg
    15661 cgctggcaac aaagcttccc ccgacggtac ctttcagcag attgttgacc cactctcctt
    15721 gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt acggtcatag gttcgggttt
    15781 cacgacgaga cacttcccag gtgaggcgct gcagttcgcc gtccgcggtt aatgtccagg
    15841 agagttgttg accgattttc aggttacgca attctttgtc ggcagcagcc agttgggtga
    15901 tatcacccat atcaatacca tactgattga gaatgctgct tagcgtatcg ccagtggaaa
    15961 caacatattc atgcacgccc gcttcaccgg cgattttgtc atccagttcg tcctggggaa
    16021 tggcttcatc ttcttgtgca gcttgatcaa tcggctcact ggcttcaggt aagagcgaac
    16081 gaatttcgtt ctgttccagc tcaatggttt tgacaattgg cgtggcatca cggtgataaa
    16141 catagggccg ccagacagcg acggccagag taagaacggt gagcgacccc aacataacgc
    16201 ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga gcgggctatc tgttgcacgt
    16261 aatcacttcc tcattaatct cctttcaggc agctcgcata ctggttggct aattgattca
    16321 ggaattctga atagcttgtt ttacccagtt tgatattcgt ccccagggga tccaacgttc
    16381 ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac cgctggcctg aactgtggct
    16441 cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt tcttatttca tgtaaacgct
    16501 gcgcgccagg ttgaatctca gggttaacgg taaaatgacc aagcggtgtc agtccgaact
    16561 gtttttcgaa gtagccgtaa gcatcgtgaa aaacgaaata acctttcccc ttgagcggcg
    16621 cgagctcgtt accaacctgc gtttcggttg aggctaattg tgcctcaaaa tccttcaggt
    16681 tggcgtcaag tttggctcga ctttgcggca taagttccac taattttcca tggattgcaa
    16741 ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg catgttgaaa tcgccgtgat
    16801 ggtgatcttc gtcacttttt tccgcgtgat cgtgatcatc atcatcgtcg tgaatacttt
    16861 tcatcagcag cggtttcaca tcttctagct gcgcaatcgt tacctgtttc gcttctggta
    16921 atttacttac cggtttttgc atgaacgctt ccatctccgg gccaacccaa acgactaagt
    16981 ccgcgttctg taagcgtttt acatccgatg gacgcagtga ataatcatgt tctgaagcgc
    17041 cgtcaggcag taaaacctgc gtttctgtta ccccatcagc aatggcagaa gcgatgaacc
    17101 caacgggttt aagcgaagcg acaacggcgg catctgcggc ctgtgttgca ccgccccaga
    17161 gagcggcgga taatgctgcg aaaagaagcg tttttttatg taacataatg cgaccaatca
    17221 tcgtaatgaa tatgagaagt gtgatattat aacatttcat gactactgca agactaaaat
    17281 taacatgaca agtctggttt ccctggaaaa tgtctcggtt tcttttggcc aacgccgcgt
    17341 cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt ttgactttac ttgggccaaa
    17401 cggcgcaggt aagtcgacac tggtacgggt agtgctcgga ctggtaacac ccgatgaagg
    17461 ggttatcaag cgcaacggaa aactgcgcat cggctatgta ccgcagaagc tgtatctcga
    17521 caccacgttg ccactgaccg taaaccgttt tttacgctta cgccctggca cacataaaga
    17581 agatattttg cctgcactga aacgtgtcca ggccgggcat ctgattaacg caccgatgca
    17641 aaagctctcg ggtggcgaaa cgcagcgtgt actgttagcg cgagcattgt taaatcgacc
    17701 gcaattatta gtgctggatg aacccactca gggcgtggat gtgaatggtc aggtggcgtt
    17761 atatgacctt attgaccaac tgcgtcgtga actggattgt ggcgttttaa tggtatctca
    17821 cgatctgcat ctggtaatgg caaaaaccga tgaagtgctt tgcctgaatc accacatttg
    17881 ttgttccggc acaccggaag ttgtttccct gcatccggag tttatttcta tgtttggtcc
    17941 tcgtggtgct gaacaactgg gtatctatcg ccatcatcat aatcatcgtc acgatttaca
    18001 gggacgaatt gttttgcgtc ggggaaatga tcgctcatga ttgaattatt atttcccggt
    18061 tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc tgggttcgtt tgtagtctgg
    18121 cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct cattacttgg cgtcgcgttt
    18181 ggtttgttgc tggacgtgaa tccattctat gcggtgattg cagttacgct gctgctggcg
    18241 ggcggtctgg tatggctcga gaagcgtcca cagctggcga tcgacacgtt attagggatt
    18301 atggcgcaca gtgccctgtc gctgggcctg gtggtcgtta gtctgatgtc taatattcgt
    18361 gttgatttga tggcttacct gttcggtgat ttactggcag tgacgccaga agatctcatc
    18421 tctattgcga ttggcgtggt catcgtggtg gctattttgt tctggcaatg gcgcaatttg
    18481 ctgtcgatga cgattagccc ggatctggcg tttgttgatg gtgtgaaatt acagcgcgtg
    18541 aaattgttgt tgatgctggt gacggcattg acgattggtg tagcgatgaa attcgtcggc
    18601 gcgttgatta ttacttcact gctgattatt cctgctgcta ctgcacgtcg ctttgcccgc
    18661 acgccggaac agatggctgg tgtcgctgtt ttggtgggga tggtggcagt gactggcggt
    18721 ttaacctttt ccgcatttta cgatacacct gcaggcccgt cggtggtgct atgcgcggca
    18781 ctgttattta ttatcagtat gatgaaaaaa caggccagct aatctttcgc tgaacatatt
    18841 tgtcggatgc ggcgcgagcg ccttatccga ccttgtatta tccctccagt gcagagaaaa
    18901 tcggtcagtt ttctctgcct gcagtccgca tgccttatcc gacctacggt tcgctatctc
    18961 tggtaggcct gataagacga ac
//
LOCUS       DAJOTB010000089        18931 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJOTB010000089 DAJOTB010000000
VERSION     DAJOTB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.089"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            883..1365
                     /locus_tag="OHN28_003085"
     CDS             883..1365
                     /locus_tag="OHN28_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCQ2298435.1"
                     /translation="MSNTSAHALLKDIWGDRKFPVDPVWIANELGLDVVETTLDDDVS
                     GALLKEPEQDPVIILNRNDSNVRKRFTCAHELGHYVKRTENGQPLEYEFVDYRGKLAS
                     QGVDSEEIFANNFAACLLMPEKEVRRLNNAGYAPVMMASYFGVSDDAIRYRLKNLGLR
                     "
     gene            1369..1746
                     /locus_tag="OHN28_003086"
     CDS             1369..1746
                     /locus_tag="OHN28_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298436.1"
                     /translation="MTSRSYAISEKLKQAARQARGEPASGSSPSAKTLTHLNDKDPAK
                     AHQRAKTKDLKTDTKLKKMFARWFIGILIGQLLVMNGIFVLIGAKLLHFSDFVINLFM
                     GGTLAEVFGIVLVMARYLFSKHV"
     gene            complement(1763..2206)
                     /locus_tag="OHN28_003087"
     CDS             complement(1763..2206)
                     /locus_tag="OHN28_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298437.1"
                     /translation="MVFAMSKSNLIAFRIPSELQDEFNRSVLASGGDKTSWLVDAIRM
                     KLGQPEKSIDSRMLGLVERMEKAAASLIAGKPNIPPKPYNETAVIKIIADTIQQGFDN
                     GRVIAERLNEAGYQTKAGKAWDKDIYSAWKRQGSNAEKLSVALRM"
     gene            2466..2753
                     /locus_tag="OHN28_003088"
     CDS             2466..2753
                     /locus_tag="OHN28_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCQ2298438.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWAEVMMLADGDKEREHAANRRS
                     ECIRKAARPPATTDRFGDLRQAVKRTHSALGMDEEARGYFRRYRDKDCQRQ"
     gene            3492..4109
                     /locus_tag="OHN28_003089"
     CDS             3492..4109
                     /locus_tag="OHN28_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCQ2298439.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(4106..5788)
                     /gene="ligB"
                     /locus_tag="OHN28_003090"
     CDS             complement(4106..5788)
                     /gene="ligB"
                     /locus_tag="OHN28_003090"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCQ2298440.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            6046..6669
                     /gene="gmk"
                     /locus_tag="OHN28_003091"
     CDS             6046..6669
                     /gene="gmk"
                     /locus_tag="OHN28_003091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCQ2298441.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            6724..6999
                     /gene="rpoZ"
                     /locus_tag="OHN28_003092"
     CDS             6724..6999
                     /gene="rpoZ"
                     /locus_tag="OHN28_003092"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCQ2298442.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            7018..9126
                     /gene="spoT"
                     /locus_tag="OHN28_003093"
     CDS             7018..9126
                     /gene="spoT"
                     /locus_tag="OHN28_003093"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCQ2298443.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            9133..9822
                     /gene="trmH"
                     /locus_tag="OHN28_003094"
     CDS             9133..9822
                     /gene="trmH"
                     /locus_tag="OHN28_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCQ2298444.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            9828..11909
                     /gene="recG"
                     /locus_tag="OHN28_003095"
     CDS             9828..11909
                     /gene="recG"
                     /locus_tag="OHN28_003095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCQ2298445.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11943..13148)
                     /gene="gltS"
                     /locus_tag="OHN28_003096"
     CDS             complement(11943..13148)
                     /gene="gltS"
                     /locus_tag="OHN28_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCQ2298446.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            13428..14819
                     /gene="xanP"
                     /locus_tag="OHN28_003097"
     CDS             13428..14819
                     /gene="xanP"
                     /locus_tag="OHN28_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCQ2298447.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14940..16649
                     /locus_tag="OHN28_003098"
     CDS             14940..16649
                     /locus_tag="OHN28_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2298448.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLKNVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(16693..17373)
                     /locus_tag="OHN28_003099"
     CDS             complement(16693..17373)
                     /locus_tag="OHN28_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCQ2298449.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(17354..18286)
                     /locus_tag="OHN28_003100"
     CDS             complement(17354..18286)
                     /locus_tag="OHN28_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCQ2298450.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(18334..>18931)
                     /locus_tag="OHN28_003101"
     CDS             complement(18334..>18931)
                     /locus_tag="OHN28_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCQ2298451.1"
                     /translation="LRAIRCGFTSVMIDGSLLPYEENVALTAEVVRLAHAVGVSVEGE
                     LGTIGQTGTSVEGGVSQVTYTDPAQAADFIARTGADTLAVAIGTAHGIYPKGMQPKLQ
                     MDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYFQKVREILA
                     KESWWDPNVIYPDAINAAREVIRHKMKLFGSLGKASLY"
BASE COUNT         4728 a         4998 c         4764 g         4441 t
ORIGIN      
        1 tagcttgggt cgggatttca aatccgatcc ccgttgatgg ggccgttaca gtacgcgcat
       61 atccgcttta tgtgaaacag gaagattttt ctgatatcac tgccacagca aaacaccaat
      121 ttcgtgacca gtacgcgcgc gaggggcatc agttgcaaaa agttttgcaa ctgcgtccga
      181 ccagtacacg cacgatggag gggagtacca taaaactgtt ctctacataa gccagagtgg
      241 tggtgatatg ttgattatcg gcgtatatgc gcacaggacg cgctatgacg atgtttgatg
      301 cgggtacacc atcacatgta tctggtgtga taaatcgcgt tacatgacat ctcatgttgt
      361 gctggtggtt atcacgacgt tctgaatctg gcctttgcct gatattttga gacaagtacg
      421 cgcgcgtagc atcgaaatta tcggatggcg tatgctgctg gcaatatgaa gaccgatagt
      481 attaaccgaa aagcgaaaga gcttctcgat aacggcaaga tcgcggcaag ggttgctgaa
      541 tttgaacaga tgtaaggaat ggaaccagag aggcgctggc ggcgaaaaaa cgccactacc
      601 agcggataaa tgtgccaatc ttttagttgg aaaaacagca tagaaggcta ccatgatatg
      661 taaagattgc tcaaaaagtt attatcaacg tctaatttca ttaaaaatac tccttattgc
      721 tgttaaaaat caaagaaatc atttaaaaac cgttcaacac cccccatctg tcttcaaaaa
      781 gatttgtgtt ggttagtgac tagttacctt ttatatgtaa atatatccac aagggaagtg
      841 gtgaaaattt cgactattta accataatca aaggaggtga acatgtcgaa tacatctgca
      901 catgccctct tgaaagatat ctggggcgat aggaaattcc cggttgatcc agtatggatt
      961 gccaatgagt tagggttgga tgttgttgaa accacgcttg atgatgatgt ttcaggcgca
     1021 ttgctgaaag agccagaaca agatccagta atcatactca atcgtaatga tagtaacgtg
     1081 cgcaaacgat ttacgtgtgc gcatgagtta gggcattacg tcaaacgaac tgagaacggt
     1141 cagccacttg aatatgagtt tgttgattac cgcggaaaac tggcaagcca aggtgtagac
     1201 agcgaagaaa tattcgccaa taactttgca gcatgtcttc tcatgccaga aaaagaggtt
     1261 cgacgcctca acaatgcagg atatgcacca gtaatgatgg cttcctattt tggtgtatcc
     1321 gatgatgcta tacgttatcg cctaaagaat ttagggcttc gatgagtaat gacgtctaga
     1381 tcttacgcta tatctgaaaa actaaagcaa gcggcaagac aagccagagg tgaaccagcc
     1441 tctggctcat ctccatcagc aaaaaccctc acacatctaa atgataagga tccagccaaa
     1501 gcccatcagc gtgcaaaaac aaaagattta aaaacagaca caaaactgaa aaaaatgttt
     1561 gctcggtggt ttatcggcat tctgataggc cagctattag ttatgaatgg gatttttgtc
     1621 cttattggag caaaactgct ccattttagt gatttcgtta taaacctatt tatgggggga
     1681 accctcgctg aagtctttgg cattgtttta gtgatggcac gctacctgtt ctccaagcac
     1741 gtctaaaaat caaaatatag ggctacattc ttagagccac tgataacttt tcagcattgc
     1801 ttccctgacg cttccacgcg ctgtaaatgt ctttatccca tgctttacca gcttttgttt
     1861 gatagcctgc ttcattgagc cgttccgcta tcacacgccc attatcaaat ccctgttgga
     1921 tggtatcggc aatgatcttt ataactgccg tttcgttata tggctttggt gggatgttcg
     1981 gctttccggc aataagcgac gcagcggctt tttccatgcg ctccaccagc ccaagcattc
     2041 gtgaatcaat agacttttcc ggctgtccca gcttcatgcg aatcgcatca acaagccagg
     2101 aggttttatc ccctccagaa gcgagaacag accgattaaa ctcatcctgc aattcagatg
     2161 ggatgcggaa cgcgatcaga ttagatttgc tcattgcaaa gaccatatca gtgagtaata
     2221 tccatacagt ataccactgt ataacactgt tatacgctca aatatcagcc agaaaataac
     2281 ggaccaatca caaagctatc gctgtgttgt tggttcagag ctgggctgtg ttggttcaaa
     2341 atgagttgat gttggttcaa catttcagaa aaaacacaat aaaaacaacg atctttacaa
     2401 attgaaccaa ctgaaccaac tgaaccaaca cctttttgct tatctatata atctgagagg
     2461 cagaaatgat aagggacagg aaagctgaag agctggagtc aaaagggcta taccggagag
     2521 ctgccgcacg atgggcagaa gtcatgatgc tggcagatgg cgataaagaa cgtgaacatg
     2581 cagcaaacag acgctctgaa tgtatccgta aggcggcacg cccaccagca actacagaca
     2641 gattcggaga tttaagacag gcagttaagc ggactcattc ggctcttggt atggatgagg
     2701 aagcgagagg gtacttcagg cgttatcgcg ataaagattg ccagagacag taatgctctc
     2761 tctctgtttt ggcaaattcc ccctcacttt gaagggatcg gagctataaa aatggtgtta
     2821 tccggcttcc tgcattgtag gttttcgaaa aaatcaatgg gttaggtcat cgtgcaaatt
     2881 tgcactctgc cagccaacgc aatttctcgt tggcgaaaat atcaggtatt tactgaacga
     2941 acggtctacc agtctattca gttaccagtt gcacatggct gtttcatgtg agaacggagg
     3001 ttttttcgga catcactatt ggttacagca atttcacgag caatacgcgc gcgcaggaca
     3061 acaagcctga acatggttac acacctcgat cgtgagggat gtagacaatg gcttctggcc
     3121 aaagcacccg aaaaaaccgt gtacataagg gtgtacataa tcaaaaaccg cattagaaag
     3181 aaaccagcta caatgcggca ttgaagccaa tactctacaa acagattcaa tatcgagtaa
     3241 gcgacatatc tccgtaggcc gttgttaatt acagcggcgc tacgcatata gtttcgtcct
     3301 catatttatt tactccatac tttttctaat gcattttact tatcacaaaa tcagaaaaat
     3361 gcattcttac tacagaaaat agcaatttca cataactaca aattatcaat ctattctctc
     3421 cttaaaaaat ctcaatcgtg acaatgcgca caaatcgcta cccttccaga cagattttta
     3481 gggagagaac catgctgtta cacattttgt atttggttgg cattactgcc gaagccatga
     3541 cgggggcgct ggcggccgga cgacggcgca tggatacatt tggcgtaatt attattgcga
     3601 ccgccacggc aattggcgga gggtcagtgc gcgatattct gctgggccac tatccgctcg
     3661 gctgggtcaa acacccggaa tatgtgatta tcgtcgctac cgctgcagta ctcaccacta
     3721 tagttgctcc tgtaatgcct tatctgcgca aagtgtttct ggtgctcgat gcgctcggac
     3781 tggtggtatt ttctatcatc ggcgcacagg ttgctctgga tatggggcac ggcccgatta
     3841 ttgccgttgt cgcggcggta accaccggcg tgtttggcgg cgttttgcgc gatatgttct
     3901 gtaaacgcat cccactggta ttccagaaag agttgtatgc gggtgtctca tttgcctccg
     3961 ccgtgctgta catcgcgctg caacactatg tttctaacca tgatgtggtg attatctcca
     4021 ccctggtatt cggctttttt gcccgtttac tggcgctacg tcttaagctg ggattaccgg
     4081 tcttttacta cagccacgaa ggtcactaag gttcaaaacc tgtgatctgc tgggcagcca
     4141 gccaactgcc cagcttcttg atttgcgcat tttccttcca ttcaataacc tgtctggcgc
     4201 gccccgatcc agtccccggc agctgctgcc agaactgttc cgtgctaaat aaaagttgcg
     4261 accaggaccg ttcatcactg gcattaagcg ccgcccgagt tagcggtatt cccattgcca
     4321 tcacccagcg agtaaaaggc tgctgacgag ccagattaaa ctgatgccat aactgcgcac
     4381 ttttactttt cgcgatcccc ggcgtgttct gtaattgctc tggcgttaat aaaagccagg
     4441 aaaagatatg ctcaaagcga tgagtctgat gcagggcgcg ccagccggcc tcaccaatgc
     4501 catcaagccc cagaacctgt tttgacccca gccagactaa gcgtgaaatg aactgttcct
     4561 gacaaacatc agaagcaaag tagcaggtca acgagttaaa gcggttttct ggtggtgtcg
     4621 gttttgtacg ttctgctccg cgccagacca catcatcaat gcgaggaatc ccctgaccgg
     4681 caaggctgac gaggatctga tcaccaggcg caatatccca ctcctgccag cgcctgacgg
     4741 aaccaatatt cacccgctgg acttttttat catccagcat gacaggtaca agtgacgcaa
     4801 ccaccgatat tttaccgctc ttacccaccg caaactgaat cgccttcact tcggcaacct
     4861 gagctacagg ttgatatttc caggccacca gccactccgc ctggcccggt agccaatgac
     4921 gggattcggg ttctttcgcc gctcgcacaa ccacgccatc ggtgacgaag ggtaattttg
     4981 ctttccacca ctcattgcgt acgtgcgcaa cctcatcagc atttttaacc gcgcgggtat
     5041 acgtctgcgt taaagtaaaa cctgcggtag ccagatcctt taaacgatcg gtcattaaat
     5101 gcggtccatc cggccatgcc cagacaaaaa cagccagtga gtttagcgta tcgctattcc
     5161 cctggcgcat catcaggcca gcaacttttg cgcgggcatt tattcccccc atttgttgtt
     5221 ggatatgccc cttacgcttg agaaatattt ccccctgaag cgtgctgttg gctaaaggcc
     5281 cgctaacggt ttgcggcaca gcggaaatta agcgaacttt ctgcgtccag tcctcgcctt
     5341 tcaggccatt gccgcgactg attgctttgt tcagtttccc gtcccgataa accagggtta
     5401 ccgcaacgcc atcaactttt ggctgcaccc aaagatcgct acgttcgcgc atccaaagac
     5461 ttaatgcgtt tttatccgcc attttgcgta cacccgtatg ggcaaccgga tgcataaccg
     5521 caccgtttaa aggcggtatc atcacatcgc gggtctcgtt cccaaaacag cgttgccact
     5581 gcgtaagacg ggcgcttaac tgatcgtaaa caccgtcttc cacctcactt ttcccttcct
     5641 tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt tcttcctgtg
     5701 ctctggctgg cgaccaggcc ggacagaccg cccacaccga tgattgccag cacaagatac
     5761 ttattaatat cgccatccat actttcatca tcacctccgc tgtagatagt caggcagata
     5821 tacaatgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga cgcttcctga
     5881 gaatcgtctt tattgcagtg aatcacaggc aaatgcggaa gcagctacgc aaaacgcaac
     5941 aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt gtataataag
     6001 ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc tcaaggcacg
     6061 ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca ggctttatta
     6121 aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg ccaaccgcgt
     6181 cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt taaagaaatg
     6241 attagtagag atgcgttcct cgaacacgca gaagtttttg gtaattacta tggcacttcg
     6301 cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga tatcgactgg
     6361 cagggtgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt tattttaccg
     6421 ccgtctaaaa tcgaactgga ccgccgtcta cgcggtcgcg gtcaggacag cgaagaggtc
     6481 attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga atatgattac
     6541 ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat tattcgcgcc
     6601 gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag caaattgttg
     6661 gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg gagcttttta
     6721 agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg ttttgacctg
     6781 gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga tccgctggta
     6841 ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga aggtctgatc
     6901 aacaaccaga tcctcgacgt tcgcgaacgc caggagcagc aagagcagga agccgctgaa
     6961 ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg gtcgcccttg
     7021 tatctgtttg aaagcctgaa tcagctgatt caaaactacc tgccggaaga ccaaatcaag
     7081 cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac acgttcaagc
     7141 ggtgaaccct atatcacgca tccggtggcg gttgcctgca ttctggccga gatgaaactc
     7201 gactatgaaa cgctgatggc ggcgctgctg catgatgtga ttgaagatac acccgccacc
     7261 taccaggaca tggaacagct ttttggtaaa agcgtcgccg agctggtaga gggggtgtcg
     7321 aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa ctttcgcaag
     7381 atgattatgg cgatggtgca ggatatccgc gtcattctca tcaaacttgc cgaccgtacc
     7441 cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat cgcccgtgaa
     7501 actctcgaaa tatacagccc gctggcgcac cgtttaggta tccaccacat taaaaccgaa
     7561 ctcgaggagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat caaagaagtg
     7621 gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaga agattctttc tgaaatcgaa
     7681 gggcgtttgc aggaagcggg aataccgtgc cgcgtcagtg gtcgcgaaaa gcatctttat
     7741 tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat ggacatctac
     7801 gctttccgcg tgatcgtcaa tgattctgac acctgttatc gcgtgctggg ccagatgcac
     7861 agcctgtaca agccgcgtcc gggccgcgtg aaagactata tcgccattcc aaaagcgaac
     7921 ggctatcagt ctttgcacac ctcgatgatt ggcccgcacg gcgtgccggt tgaggtccag
     7981 atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca ctgggcttat
     8041 aaagagcacg gcgaaaccag cactaccgcg caaatacgcg cccagcgctg gatgcaaagc
     8101 ctgctggagc tgcaacagag tgccggtagc tcgtttgaat ttatcgagag cgttaaatcc
     8161 gatctcttcc cggatgagat ttacgttttc acaccagaag ggcgcattgt cgaactgcct
     8221 gccggtgcaa cgcccgtcga cttcgcttat gcggtgcata ccgatatcgg ccatgcctgc
     8281 gtgggcgcac gcgtcgaccg ccagccttac ccgctgtcgc agccgcttac tagcggtcaa
     8341 accgttgaaa tcattaccgc accgggtgct cgcccgaatg ccgcgtggct gaactttgtc
     8401 gtcagctcga aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa gcgtgatgat
     8461 tctgtaagcc tgggccgtcg tctgctcaac catgcgttgg gtggtagccg taagctcaat
     8521 gaaatcccgc aggaaaatat tcagcgcgag ctggaccgca tgaagctggc aacgcttgac
     8581 gatctgctgg cagaaatcgg ccttggtaac gcaatgagcg tggtggtcgc gaaaaatctg
     8641 caacatgggg acgcctccat tccaccggca acccaaagtc acggacatct gcccattaaa
     8701 ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gcccaattcc tggcgacccg
     8761 attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc ctgccgtaac
     8821 atccgtggct accagaaaga gccagagaag tttatggctg ttgaatggga taaagagacg
     8881 gcgcaggaat tcatcaccga aatcaaggtg gagatgttca atcatcaggg cgcgctggca
     8941 aacctgacgg cggcaattaa caccacgacc tcgaatattc aaagtttgaa tacggaagag
     9001 aaagatggtc gcgtctatag cgcctttatt cgtctgaccg cccgtgaccg tgtgcatctg
     9061 gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac ccgaaaccga
     9121 aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct cgccaggcgg
     9181 cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt ttctgcgatt
     9241 attcgtaccg cagatgccgt tggcgtacat gaagttcatg ctgtctggcc tggcagccgg
     9301 atgcgcacca tggcttcggc agctgcgggt agtaacagct gggtacaggt gaaaacgcac
     9361 cgcaccattg gcgatgccgt cgctcatctg aaaggccagg gcatgcagat tctggcaacc
     9421 catctttctg ataacgctgt tgatttccgc gaaattgatt acactcgccc gacctgtatt
     9481 ttgatgggac aggagaaaac gggcatcacg caggaagcgt tggccctggc ggatcaggat
     9541 atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc ctcagccctc
     9601 attctttacg aagcccagcg ccagcggcaa aatgcgggca tgtacctgcg tgaaaacagc
     9661 atgttgccgg aagccgagca acaacgcctg ctgtttgaag gcggctatcc ggtgctggcg
     9721 aaagtcgcaa aacgcaaagg tctgccctac ccccacgtta atcagcaagg cgagattgaa
     9781 gccgatgctg actggtggtc caccatgcag gccgcagggt aagcgcgatg aaaggtcgcc
     9841 tgttagatgc tgtcccgctc agttccctaa cgggcgttgg cgcagcgctt agtaacaagc
     9901 tggcgaaaat caacctgcat accgtacagg atttgctctt acaccttcct ctgcgctacg
     9961 aagatcgcac acatctctac cccatcggtg aactactgcc gggtgtttat gccacggtgg
    10021 aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg acctgccaga
    10081 tcagcgacgg ttccggcatt ctcactatgc gctttttcaa cttcaatgcg gcaatgaaaa
    10141 atagcctggc gacaggccgc cgcgtgctgg cctatggcga agcaaagcgc ggcaaatatg
    10201 gtgcggagat gatccacccg gaataccgcg tacagggcga tctcagcacg ccagagttac
    10261 aggaaacgct cacgccggtt tacccaacta cggaaggcgt aaagcaggct acgctgcgta
    10321 aattaaccga ccaggcgctg gatctgctcg acacatgcgc cattgaagaa ctcctgccgc
    10381 cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg caccgcccac
    10441 cgccgacgct acagcttagc gatctcgaaa ccgggcagca tccggcacaa cgccggctga
    10501 ttctggaaga gttgctggcg cacaacctca gcatgttggc gttacgtgcc ggtgcacaac
    10561 gttttcatgc tcagccgctg agcgccaatg acgcgctgaa aaataaactc ctcgccgcct
    10621 taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag cgcgatatgg
    10681 cgctggatgt gccgatgatg cgtctggtgc agggcgatgt aggttccggt aaaacgctgg
    10741 tcgccgccct cgccgcgttg cgtgcgattg cgcacggcaa acaggtggca ttgatggcac
    10801 caaccgaatt actcgccgag cagcacgcca ataacttccg taactggttt gcaccgctcg
    10861 gtatcgaagt aggctggctc gccggaaaac agaaaggtaa agcacgactg gctcagcaag
    10921 aagccatcgc cagcggtcag gtgcagatga ttgttggtac gcacgccatt ttccaggaac
    10981 aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt tttggcgtgc
    11041 atcagcgtct ggcgttatgg gagaaaggcc agcagcaggg cttccatccg catcagttga
    11101 tcatgaccgc cacgcccatc ccccgcacgc tggcgatgac tgcgtatgcc gatctcgata
    11161 cctcggtgat agatgagctg ccaccaggcc gcacgccagt gactacggtc gctattcctg
    11221 atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgtatgact gaagggcgtc
    11281 aggcgtactg ggtttgtacg ttgattgaag agtcggaatt actggaagcg caggcggcgg
    11341 aagcgacctg ggaagagttg aaactggcgc taccagagtt gaatgttggc ctggtacacg
    11401 gacggatgaa acccgccgag aaacaggcgg tgatgacatc gtttaaacaa ggcgagctac
    11461 acctgctggt tgccactact gtcattgaag tcggtgttga tgtacccaat gccagtctga
    11521 tgattatcga aaacccggag cgtctgggtc tggcgcagtt acaccagctt cgcgggcgtg
    11581 taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg ctctccaaaa
    11641 ccgcgcaaat ccgcctgcag gtactacgcg acagcaacga tggttttgtg attgcacaga
    11701 aagatctgga gattcgcggc cctggcgaat tgttaggtac acgtcagacg ggtaatgctg
    11761 aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt cagcgcctgg
    11821 cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cctgatagaa cgctggatgc
    11881 cagaaacaga acgttactcg aatgcgtaaa aagcggcggt gagaagaccg ccgcttcatc
    11941 ggttaaccgg caaaaatcgg caacatcaaa tacagcttaa tcaccagcgc attgacgata
    12001 tcgataaaga acgcaccgac catcggcacg accaggaacg ccatgtgcga cgggccaaag
    12061 cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag gccaaaacca
    12121 cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg ccaggtaacg
    12181 aagatggcgt acaacgccat gaagatggtc tgtaccacca gaatcgccag catcggcaac
    12241 gccagcgaag ccagctccca cagtttcagc cccatcagcg ccatcgccag gaacaacgac
    12301 aggcttacgt tacccagcac ggataccgct cgctcaaaga cgcggtaaaa gcccatcatt
    12361 gacagaccgt tgctcagaat cacgccaaca aatagtacac agacgaaggt cggtagttca
    12421 aaagcagtac cagccaaaag ttgcgcaaca attttcccca ccgtcaggca gatagcaatc
    12481 agcgcgatag tttcaatcag caccagcgag gtgatcatgc ggcccacatc cggcttttcg
    12541 aacgccgtcg gaacttcctg gtcatccgga ataccgttcg gtgtggtgga gtgtttcacc
    12601 agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccaaaccgaa cgttgcacag
    12661 gccatcgcca cttctgtcgc gttggtgaaa ccataacgtt caatgaacaa tttactccat
    12721 gcagcacccg taccgtgacc gccggaaagc gtaatagaac cggccagcag ccccatcagc
    12781 ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat caccaacagg
    12841 ccaacaacca caatcaggaa aacccccacc acacgcccac cagctcgcag actggcaagg
    12901 ttggcgttca ggccaatggt ggcgaagaaa gccagcatca acggatcgcg cagggacata
    12961 tcaaagttga cttcccagcc catgcttttt ttcagtatca gcagcgccag cgccactaat
    13021 aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac ggaatggacc
    13081 aacttgcgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa agtatcgaga
    13141 tgaaacatag ttactccttt gtgtccgcat cactctttca tactcgtcat acttcatcgc
    13201 gcatttgcag taaggcgaaa tatgacgagt atcgagtcat gctttcctgg ttcgaattct
    13261 tcgctgaaaa tagaaaaatg gcatggattt taagcagaaa acaactaaaa gttatataaa
    13321 aaagtacatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt tgcttttctc
    13381 tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcgatg tctgtttcca
    13441 ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa ctgatttacc
    13501 gtcttgaaga tcgtccgccg cttcctcaaa ctctgtttgc cgcctgtcag catttgctgg
    13561 cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg ggtttaccgg
    13621 cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg gcatcgatta
    13681 ttcaaattaa ggcctggggt ccggttggct ccgggttgtt gtctattcag ggcaccagct
    13741 tcaactttgt tgctccgctg attatgggcg gcacagcgct gaagaccggt ggtgctgatg
    13801 ttcctaccat gatggcagct ttgttcggca cgttgatgct ggcaagttgt accgagatgg
    13861 tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt tctggcgttg
    13921 tggtgatgat tatcggcctg tcgctaattc aggttggact gacctccatt ggcggcggtt
    13981 acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg gcaggcgtgg
    14041 tcttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc gtggcctcac
    14101 tggtgattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg ttgccagaaa
    14161 gcaacgaacc gatgacacaa gaactgatta tggtgccaac gccgctctat tacggtcttg
    14221 gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact tcgctggaaa
    14281 ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc ggtccgctgt
    14341 acatgaaacg cctgaaaggc ggcgtactgg caaacggcct gaactcgttt gtctcggcgg
    14401 tatttaatac cttcccgaac tcctgcttcg ggcagaacaa cggggtgatc cagttaaccg
    14461 gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg ctgggtctgt
    14521 tcccggcagt gagcggtttt gtacaacaca ttccagaacc ggttctgggc ggcgcaacgc
    14581 ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt gagccgctga
    14641 accgccgggc gattctgatt atcgcgctgt cgctggcggt cggtctgggc gtgtctcagc
    14701 agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc tccgggattg
    14761 ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa aaacagtaat
    14821 tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca ttcccgcctt
    14881 gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga tggaagacca
    14941 tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg gtgatcgctg
    15001 gcctctattt tcttctgcaa actcgctggg gagcagaaca tatcagcgca tgggtttccg
    15061 agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc gcgccatctc
    15121 atatcgtgct gaagaacgtc acgtttggcc gtgatggcca gcccgcgacc ctggtagcaa
    15181 aaagtgtcga cattgcgcta agcagtcggc aactgaccga accacgccat gtcgatacca
    15241 tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta ccgttcaaag
    15301 ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa tggaaactga
    15361 gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt aaagtgctgg
    15421 gtacgaaggc acagattcag tttagtgccg gatcgctttc gctcaatgat gttcctgcca
    15481 ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact aacctgggtg
    15541 ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgctgac ggcagctggc
    15601 aagtggaaaa cctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg ctaaccgact
    15661 tctttgcacc attacgctct gtcccgtcgt tgcagattgg tcgcctggaa gtcatcgacg
    15721 ctcgtttgca aggtccggac tgggcggtgg ccgacctcga tctcagcttg cgcaacatga
    15781 ccttcagtaa agatgactgg cagacacagg aaggtaaact gtcgatgaac gccagcgagt
    15841 ttatttatgg ctcgctgcat ttatttgatc cgattataaa cgcggaattt tccccgcagg
    15901 gcgtagcact gcgccagttc accagccgct gggaaggggg tatggtcaga acgtcaggaa
    15961 actggcaacg cgacgggaaa acgctgatcc tcgatgatgc ggcaattgcc ggactggaat
    16021 ataccttgcc caaaaactgg caacagttgt ggatggaaac gacacccggt tggttaaaca
    16081 gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc gaccctgact
    16141 tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa tctgacgctg gttaccgatc
    16201 ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgca acgtttaatc
    16261 gtgttgatgt ccgtcgtccg tcgctggcgc taactgccaa cagcagcacg gtgaatatca
    16321 gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgccagt gtttcacaaa
    16381 cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg aatattttgc
    16441 aacattgggg atggcctgaa ttaccgttga ctggcgacgg caatattcag cttaccgcca
    16501 gtggcgatat tcaggtcaat gtcccgctga aacctacggt tagcgggcaa ttgcatgccg
    16561 tgaatgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtagtt tccagtggtg
    16621 aagttacgtc gacggagccg gtgcagtaaa aataaggacg ctagcggata tcagcgctag
    16681 cgttccccgt tattagcgca aaattccata ctcaccgcac aataaccatt tcaccggttc
    16741 atcttcttct atcgccggaa aacgtcctcc cggctcaaga aaataattat cgtgttggtc
    16801 atcatttacc atcgagattt cccagccgag aacaaacgct ttttccgccc agaagcgatg
    16861 atacagacgc ggcggcaggc aaatgccctg ccccggcttt agcaccagcg tttcaccagg
    16921 tttcatggta cgccgctgac catcaacact cacggtaatg actcgttgat catcacagag
    16981 attggcttcg ttggcccagg ccaactgcat gcataagtct ccaccgcccc gattgaggat
    17041 atcttccatt ttgtgggtgt gaaaatgcca gggagtctgc tgatcttgtt ggatctgcaa
    17101 cattttctca gcatacggtt ttggatatgc cgccgacccc agcgcgccgt tgcgcatcgt
    17161 tagcagtgtt agtcccgttt ggttgaaatc accacgacca aaatcggtga tatcccaacc
    17221 gagctgtaag tcgaccactt cttgccagtt atcccgttcc cgctgcagcc agtcatccac
    17281 cgtaaaatag gcgaactctg gcaaacaaac gcggaaggtc tgcgcaatgg catgtgcttt
    17341 atcaataaca taattaattt gtgaacgttt cattggcttc tccgttgcgc atagcaagcc
    17401 aggcagcgcc acgcattccg ctactgtcgc cgtgctgtgc ttttacaatg ggagtggtga
    17461 attgatcggt gaaaaccagt ggagcgactt ctgcgttcag atcggtgata agcagttcca
    17521 cattcgaaag cccgccgccg atcacaatca cgccagggtc aacaacgtta acgatggttg
    17581 ccagcgtgcg ggcaagctgt tggcgaaaac gtgccacctg ctgcatggca caagcatcac
    17641 cacgttgtgc cagggtgaca atcgccgcag gagtgagcaa gttaccagtc atctgttgat
    17701 agcgttcact aaaaccgctg ccggaaacaa acgactccac gcagttgtgt ttgccgcaat
    17761 agcaactgac tgatggacca tcttcctgct ccgtatagcc tggcaacgtg atatgaccgc
    17821 attcggcagc atttccccag gcccctataa acggtcgttg cttgatggca atgccaccgc
    17881 cgcagcccgt accaagcgta ataccaaata ccacgtcata atcttgcccg gcaccgtcgc
    17941 aagcttctga tagcgcgaaa cagttaccat cattggctat caccaccggt tgcccaagca
    18001 attgctgcaa atcagcctgt aacgcatgac cgttaatcac ctgaatattc gcgtttttaa
    18061 tcaggccagt gagtggcgag atactgccgg gtaaggcgat ccctgtcagc atcggtcgtt
    18121 gaacgtcccg cctaatctgc tcgataagcg ccacaacgca tgagacaaat tgttgatatg
    18181 tcgacttttg cgtcgggcag cggtaacggc gaatctccca gccatgcgca tccatgacaa
    18241 cggcggcgat tttggtccca ccaatatcca accccagata gtgcatatct cgttccttgt
    18301 tgcgtaaaag gcgggtcacc ccgcccttct ggcttagtac agtgatgctt taccgagcga
    18361 accgaacagt ttcattttgt ggcgaatgac ttcccgcgcc gcgttaatcg catccggata
    18421 gatgacgttc gggtcccacc agctctcttt agcgagaatt tcacgcactt tctggaaata
    18481 ggcgtatttc atatcgctgg agatattgat tttgccgacg ccgagcgtaa ctgattcagc
    18541 aatctccgcg tccgggtttg ccgaaccacc gtgtagcacc agcggaatat caatacgtcc
    18601 ggcaatgtcg cgcaaaatgt ccatttgcag cttcggctgc atcccttttg gatagatgcc
    18661 atgcgctgta ccaatcgcca cagccagtgt gtcggcacct gtacgagcaa taaaatcggc
    18721 ggcctgcgcc ggatctgtgt aggtcacttg tgaaacacca ccttctaccg acgttcctgt
    18781 ttgtccgatg gttcccagtt caccctcaac cgacacgcca accgcgtgag cgaggcgaac
    18841 cacttctgcg gtcagcgcca cgttttcttc atacggcagc agcgaaccgt cgatcattac
    18901 tgaggtaaat ccgcagcgaa tcgcacgcaa a
//
LOCUS       DAJOTB010000090        18929 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJOTB010000090 DAJOTB010000000
VERSION     DAJOTB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.090"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            170..1141
                     /locus_tag="OHN28_003102"
     CDS             170..1141
                     /locus_tag="OHN28_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HCQ2298452.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFGTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            1205..1678
                     /locus_tag="OHN28_003103"
     CDS             1205..1678
                     /locus_tag="OHN28_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2298453.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            1675..2946
                     /locus_tag="OHN28_003104"
     CDS             1675..2946
                     /locus_tag="OHN28_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCQ2298454.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(2989..4653)
                     /gene="tsr"
                     /locus_tag="OHN28_003105"
     CDS             complement(2989..4653)
                     /gene="tsr"
                     /locus_tag="OHN28_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCQ2298455.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVNTVTPATPRKMAVADSEENWETF"
     gene            5030..7180
                     /gene="btsT"
                     /locus_tag="OHN28_003106"
     CDS             5030..7180
                     /gene="btsT"
                     /locus_tag="OHN28_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCQ2298456.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFISALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            7298..7501
                     /locus_tag="OHN28_003107"
     CDS             7298..7501
                     /locus_tag="OHN28_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2298457.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            7512..8468
                     /gene="yjiA"
                     /locus_tag="OHN28_003108"
     CDS             7512..8468
                     /gene="yjiA"
                     /locus_tag="OHN28_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCQ2298458.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(8502..8804)
                     /locus_tag="OHN28_003109"
     CDS             complement(8502..8804)
                     /locus_tag="OHN28_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HCQ2298459.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(8943..9677)
                     /locus_tag="OHN28_003110"
     CDS             complement(8943..9677)
                     /locus_tag="OHN28_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HCQ2298460.1"
                     /translation="MTTKLLDLSWNANAIPAMQIVGTRAALFTFKTHMAGFVWDAAQL
                     ENNPYTFVEVKTLLDGVTVGGHKVSDTEQVLNLADSAKKLIELVQFGRFDLDKKTFTL
                     LNGIIARNEALEWGVFRGEGEELHYNARVHLGELGTHFPPATEAGAPELNRIFAAGVA
                     QIKEMPPFEGALAMFLFGAYFQFFFDGNKRTSRHMMNGWLMRHGYNPISVPAARALEF
                     NSKMVRFYHSKDASEMMAFLVDCYQV"
     gene            complement(9721..11820)
                     /locus_tag="OHN28_003111"
     CDS             complement(9721..11820)
                     /locus_tag="OHN28_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCQ2298461.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWSEEQCRQLWDDVIRA
                     GQRDDIPAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLLEALSRHLDDDEV
                     MDGFSAMKLRNYYLLNPYESGERGFKLLLTQTDRESLLALIRQKPLAENCSLRVKENF
                     EFFHEQIGKLHGNFTALCQGLSKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNYVLMGLEPEHQSQLYDDYWRPMELAFGQKGYTDYFDSFMRNYLTVKTGEIPRIGDV
                     YEAFKRYARKPGVMDSGMEALVADIRTYADYFCAMALDSEKDKILKMAFQDLRELKVD
                     AAWPFLLVLYHDYKQGILSAADFLSATRLIESYIFRRAVCAIPTNSFTKTFATFYKVL
                     DKERYLESIAAHLLELPSYRRFPDDDEFQRELKTRDLYNFRNRSYWLRRFENHDRKER
                     VYVDTYTIEHIMPQNPNLSAKWRDDLGPEWKRIHKKWLHTLGNLTLTGYNSSYSDRPF
                     AKKRDIKGGFKESPLRLNTGLGQCEIWDRAAIQKRAERLAELAIDVWAIPKLSEDVLA
                     RYRTVVERGTGYVLTDFPQLEEGTHIRVLFDSLSDAVMTLDAGVTREILKLYVAFKAE
                     TNFVDVVPQKSRLRLSLNMPFYELVDPKCMAKDVTDIGRWGNGDVEVGFSDLSELPYV
                     MGLIRQAFEKQMDNAMA"
     gene            12105..14450
                     /locus_tag="OHN28_003112"
     CDS             12105..14450
                     /locus_tag="OHN28_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCQ2298462.1"
                     /translation="MSSTNFSQIAAFLWSVADLLRGDFKQSQYGRIILPFTLLRRLEC
                     VLETTKDAVITEAKKVKAMKLPEEAQEKMILRATNGLTFFNASAMDLSKMGQNGIQDN
                     LENYIQSFSSDAREIFEHFKFSEFVGQLADANLLFKVVQIFAKADLYPEHVTNHDMGL
                     VFEELIRRFAESSNETAGEHFTPRDIVNLTTSLVFFDDDDALNKDGIIRTIYDPTAGT
                     GGFLSSGMEFVHQQNPNAVMRAFGQELNPESYAICKADMLIKGQDVSLIKLGNTLSND
                     QLPAEKFDYMLSNPPFGVDWKKIETDINDEHKLKGADGRFGPGLPRVSDGSLLFLLHL
                     ISKMRDAKSGGGRIGIILNGSPLFTGGAGSGESEIRRYILEADLLEGIIALPTDMFYN
                     TGIATYVWILSNKKAPERKGKVQLIDGSNLCGKMRKSLGSKRNILGEEDIGLITRTFG
                     DFEPVATTTLAALGLEKAPEQKSSRGRQPATTKTEAAKTFASKVFHSTDFGYRRITVE
                     RPLRLSAQISDDAIATLRFAPKPFNAPMAQLYDAFAFQWQDGNYGDLTAVESEARAIL
                     KADFSELKEKQIKDLLDSKLWLAQRGLMEKARRIQAAMGMQAGGKDTVSNDFNQFQLT
                     LKEALRTAGVKLDAKENKQFIDAITRKNPDAEPVVSKVLKEAAQPLYGACEYNGKVVE
                     FEPDGDLRDNENVPLNPAVSTSELIEGYFKAEVLPHVADAWINADKRDAKDGDIGIVG
                     YEIPFNRHFYVYQPPRPLEEIDADLDAVSAEIMKLLQEVHS"
     gene            14450..15757
                     /locus_tag="OHN28_003113"
     CDS             14450..15757
                     /locus_tag="OHN28_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCQ2298463.1"
                     /translation="MAGLNKYQAYPEYRDSGMEWCNELPLNWKKTKLRWLSNIFAGGT
                     PSKNVIDYWENGTVPWISSGAVNQGYIVEPSTYISNAALENSSAKWIPKGALVVALAG
                     QGKTKGMVAQLGINTTCNQSMAAIVLYKKNQSRYIFWWLISNYQNIRNMAGGDLRDGL
                     NLELLGDIQCPKPRNDESSKIALFLDHETAKIDNLIEKQQQLIELLKEKRQAVISHAV
                     TKGLNPDVPMKDSGVEWLGEVPKHWHICKLKWFANLKSGDFITSNSIEPEGNYPVYGG
                     NGLRGYYSYFTHNGEYVLIGRQGALCGNINYAIGKFWASEHAVVVTPNERAVTIWLGE
                     LLRIMNLNQYSVSAAQPGLAVERITDLYIPIPPYQEQVNIGTYISKYISLDKKLIEHS
                     TDNIELLKERRTALISAAVTGKIDLRNWTAPISEAETPTEASA"
     gene            15754..>18929
                     /locus_tag="OHN28_003114"
     CDS             15754..>18929
                     /locus_tag="OHN28_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCQ2298464.1"
                     /translation="MSHDITKEVNLQNDIIAQMVEKGWIVGKGDAYDRERALYAQDTL
                     NFVQTTQPQEWEKFSRVYPNDTERHFLDALVAQLKKADSNATDIQSRTWGTLGVLRHG
                     LKIRNARFSLCQFKPEHNLNPETLARYQQNICRIVPELVYSPYASNSVFAETGVKAKK
                     WRIDLVLFINGLPIATLELKSEFKQAVQNAIAQYKKTRLPKDPATNKPEPLLTFKRGA
                     LVHFAVSQYDVFMTTKLAGDSTFFLPFNKGTKDGGAGNEIPEDTHRYATDYLWNEVLL
                     PDNLLKILASFVHLQIEEKEEWNGMKAKKESLIFPRYHQWDVVNKLLEAATIEGTGHK
                     YLIQHSAGSGKSNSIAWTAHQLSRLYDVNGEKQFHSVIVVTDRTVLDDQLQDTIWQFE
                     HADGVVGRINNREGDGSKSEKLANALEKSQPIIIVTIQTFPFVLKAIENSVSLKQRKY
                     AVIADEAHSSQSGSTARQLKEVLMSEEMDDDLVLSSEDILDATIAARKGSSNLNYYAF
                     TATPKSKTLELFGRCPRPDEPASKTNKPEAFHVYSMRQAIEEGFILDVLKNYTNYKVA
                     YQLLQKLEDKDREVDSKRAKVKLNQWVTLHDHNISQKVKVIVEHFRKHVMHLLGGQAK
                     AMVVTSSRKAAVRYKLAFDKYISENNYQKISAMVAFSGEVEFQEGDPNSLALLNQKFT
                     EINMNPGLKGRDMRKAFDSDDYQVMLVANKFQTGFDQPKLCAMYVDKALGGVECVQTL
                     SRLNRTYPGKAESGTFVLDFYNDPDEILGAFQPYFQTAELTDVSDPQQVFDLFEKLRA
                     TQIFQWHEVEQFCEAYFIKNKSNAAISNICKPAVERWKKRYLMAIDAYLTAKEMFERT
                     KKTGDTVLITNAENSFKACKQEKDRLEIFKKDLGSFVRFYEFMSQIVEYDDKDLEKLS
                     LFARHLRPLLHEQRLEEDEIDLSNVEMSHYRLSKIHEQDLRLQEETPDYTLDASNNIG
                     TAKAKDKKEEFLSQVLSRMNELFISDHLTDKDMISNAFAVYNKITENENVMTQIKNNP
                     PEQAILGDFLQAVDNAVMDSNDARQEFM"
BASE COUNT         4881 a         4782 c         4611 g         4655 t
ORIGIN      
        1 taaaaaccga ttaaaagcag caaagtatta cccggtgcac gaatttatga tacatctgca
       61 gccattctgc tattcgctga ctacttttca gtaatgactg ctttttcata aggtttcact
      121 cccgacaaca atgttggagt tttatttcct tagcatggag taagtaaaga tgagcaaaat
      181 aatagcgatg ctaattactg tcagtaccct ttttttaccg tcaataatcc aggcaaaacc
      241 tatttcaata aaagttgcct atgaaaataa ccctggagaa cccctggatg tggtcatgcg
      301 ttactgggca gacctattaa ataaaaaaag taatggcgaa ataacacttg cgctctatcc
      361 cagctcccaa ttgggatcaa aacaggatgt caccgagcaa gccatgatgg gcatgaatgt
      421 cataaccttg agtgatgtgg cttttcttgc cgattatgaa cccgatttgg ggattttgtt
      481 tggaccgtat ctgactgacg atccgcaaaa actgttcaaa atatatgaaa gcgactggtt
      541 taaacaaaaa aatgaaagtc tgaagaaaaa gggaattcat gtggtaatga ataattatct
      601 ctatggcact cgacagatta tttcgaaaaa acccattcgc accgttgacg atctcgcagg
      661 tttaaaaatt cgcgttccta ataacgtcat gcaaataaaa gccattcagg cgatgggggc
      721 aacaccaact cccatgccat taggcgaagt ttatcccgca ttaacccagg gcgttatcga
      781 cggcgtagaa aacccaattt cagtgctgca agggcaaaaa ctgtatgagc aggcaagata
      841 tctgacaatg gttaactacc tgacaaatac ctcagtgtgg ataggtggtg aagccttctt
      901 tggcaccctt tcaccagaac aactggagat gatccaccag accggctatg aagcgggtgt
      961 ctatagccag aaactcactc ttgagcgtga tgccgaaatg ttgaaaacca tgcaggcagc
     1021 cggcgtcgaa gtgatctatc ccgatactgg accattccag aagaaagcac gcgaagttta
     1081 ctctcaattc ccggaatgga caccaggtct gtacgaaacc attcaacagc aactgcaata
     1141 agcattaatg ctcagcgtcc tgcctcatct ctcttaggca ggacacgcac taacaggagg
     1201 tttcatgcgt ctttttggca aagtggcaga atttagtgct gcctttgcgc tattcgttct
     1261 ggttgtggtg acaattggag cggtatttat gcgttatttc atcggccaac cgcttcaatg
     1321 gacagaagaa atgtcgggaa tgctgatgat ttgggtggtg atgttaggtg gagtggtggc
     1381 ggaacgtgac agggcgcacc tgaccattcc cttcctgatg gagatgcttc caggcaaact
     1441 caggcgtgtt attgcagtac tggtcgccgt tctctccatc gcactgctgc tttatatggc
     1501 ctggctgggc taccgactag ccgaaatggc gcagtttaaa gtgacacaaa ttctgaaggt
     1561 ttcatggttc tggatcgatc tggcggtccc tgtcggcgca ctggcaacgg ctatttatac
     1621 cctctactgg ttaataaacg atacaaaaca atccgatagc ggagacaaaa tgcaatgagt
     1681 tggttattga ttattccgct aatgatcgtc tgtttgtttc tcaacattcg ggtctatctg
     1741 gcgatgttta ttggtatcat cgcttacttt ttattcttct ccagcgtacc gattgaaatt
     1801 gccgtgcaaa gactaatcgc gccaacgcaa agcccatcat tgctggcaat tccgtttttt
     1861 atcttgctag gcacactaat gagctacacc ggcattgccg aacgcatcct gcacattgcc
     1921 aacgttctgg tggggaaaat gcgcggcgga ctgggcgtgg caaacattct cgtcagcacc
     1981 atgatgggcg gcgtcagcgc ctctaacctt gctgacgcgg cgatgctgtc gcgcatgatg
     2041 gtgccggaga tggagcgtaa aggctacaat cgcgcgtttg ccgccgccat taccgctggt
     2101 ggctcgttag tcaccccgat cataccgcct ggaatagctc tgattattta cggtctggtt
     2161 gccgacgtct ctatcggaaa aatgtttatc gccggactgg tacctggttt gctatgcgca
     2221 gtcgtactga tgtttaccgt ctaccttgtc gcccgcaaaa ctaacgctaa accctcgcgt
     2281 gaaagctggc ctacgggcaa agaggtggtg ttctcgctgg gacaggcctg gcctgcgctg
     2341 ttcctcattt ttgctgtggt gggtggtatt cgcgctaaca ttttcacccc aacagaagca
     2401 ggtgcagtag cggtgctaat cgtgctggcg attggctttt ttatctatcg tgaaatgcgg
     2461 atttctcatg tcgtcaaagc attgggcgaa acggcacgcg caaccgcatc ggtgatgctg
     2521 gtaatcatgg ccagcgccgc attgggatgg attttctcaa tggaacatgc tggtgtcgca
     2581 gtggcgaatt ttgtgacttc gctgaccgaa aacaaataca tgttcctgtt gattatcaac
     2641 atattgctac tgacgctggg gatgtttctg gaaggaaacg ccattttgct gatcctggta
     2701 ccactattaa aaccggtggt cgaacacttt ggcatcgatc cggtgcattt tggcatcatc
     2761 atgatcttca acctgtcgat tggcgcattc acgccaccgg taggtaccgt gatgctgctg
     2821 gtatgtaata tcacgcaagt ttcggtggga aatttcttta aacaatccct gcccctactg
     2881 gcggctctac tgttaatgct aatgctggtg acatatatcc cggcgatttc actgttcctg
     2941 gtttaataat aaaaacgccg gatgaaatac tcatccggca tcattacgtt aaaacgtttc
     3001 ccagttctcc tcgctatctg ccacggccat tttgcgcggc gtagctggcg tcacggtatt
     3061 taccacagcc gatgcttcac gctgctgttg ctgctgttgc tgaatccgga acactgccac
     3121 tgcttcggtc aggcgactgg cctgctcttc cagcgcggcg gcggcggcgg cagattcttc
     3181 caccagcgcg gcgttctgtt gagttacccg gtccatctca gcaaccgcta agccaacctg
     3241 atcgatacca cggctctgct catcagaagc agaagcaatt tcgcccataa tgtccgtcac
     3301 gcgggtcacg gcactgacaa tctccgccat tgtttccccg gcgctttcga ccagcgtaga
     3361 gccaacatcc actttcccca ccgagtcttc aatcaggctt ttaatttcac gagccgcctg
     3421 agcgctacgc tgggccagat tacgcacttc tcccgcgacc accgcaaaac cgcgtccttg
     3481 ctcacccgca cgcgcagcct caaccgccgc gttcaaagcc agaatattgg tctggaaggc
     3541 aatgccgtca attacgctga taatatcggc gattttctgc gaactggtgg agatatcgcg
     3601 catagtctgc accacgttat ccaccacttt gccgccgcgt tgcgccgttt cggaagcact
     3661 taacgccaga tggctggcct ggcgcgcatt ttcggcgttc tgtttcaccg ttgcggtcag
     3721 ttgctccatg ctggctgccg tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga
     3781 gagatcgtta ttgccggtgg cgatttcgct ggcaccgcta taaatggcat tggccccgtt
     3841 gcgcacatca ccgacggtac gcatcagctc tccctgcata tggcgcaaac tctctgccag
     3901 ttgccccatc tcattagagc catccacctc aatcggtttc accagatcgc cgcctgcaat
     3961 atgacgaatg ctgtcaatca ggcgattcat tggcgcgacc aacgaggctt taataccgaa
     4021 ccagacggcg aagatgaccg ccagtacgac gatcatcacg cctaccagaa tccacatcgc
     4081 ctggctgtag gaggcattgt tatcgctgac ggcgatatca tagagccgat cgttttgctc
     4141 catgtaagcc acatactgct tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa
     4201 gaactcgttg attttgcctg cacctaacag ttgtatcagc tccgccagcg cattgtgata
     4261 aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg
     4321 caacgcttcg taatccgccc agtttttttc cgcctgtttc agcgaaatac tggcactctg
     4381 catcagctca gcaacggttg aaccgctacc aatattgttc tgatccatca tgtagcggat
     4441 acccgcgcgg ttgagggtgt tacgcgtctg caacaacgcg acccagctgc cattcagcgt
     4501 ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt tctttgtcgt tctttaaggc
     4561 attaaagaac agaccgcctg acgtcagttg taaaaggcca aaaacggcca aaaccagcag
     4621 taggctggtc acaattttga tacgttttaa catggtttct ctttcctgtg gaccgatgca
     4681 agattttcgg ccaggaaagg aaaaacttta tgaatatccc gcggagatga cgccgagtaa
     4741 ttttattcac cccctgaatt taaaaagcca ttatttacat aaattattcg caatataaat
     4801 atgtgatata aatcacatat ttatggtcac ttaaacgacg cctttgccac tcaccgccat
     4861 tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt tacgcgcgcg
     4921 taactctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa
     4981 cctagaacgg cttcggccaa ctattaatca atacatgcca ggttttacta tggatactaa
     5041 aaagatattc aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc
     5101 ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt
     5161 gtcggtgtat ctggtggctt atcgctacta cagtctgtac atcgcccaga aggtgatgaa
     5221 actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc
     5281 gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ccggtccgct
     5341 ggtgggtccg gttctcgccg cgcagatggg ttacctgcct ggcacactgt ggctgctggc
     5401 gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct cctctcgccg
     5461 taacggtgca tctctcggtg agatgatcaa agaagagatg gggccagtac cggggactat
     5521 cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg cgctgattgt
     5581 ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat
     5641 tgcgctgttt atgggtatct acatgcgctt tattcgtccg gggcgtgtgg gtgaagtctc
     5701 tgtcattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga ttgctcacga
     5761 tccgtactgg ggtccggcac tgacctttaa agacaccacc attactttcg cgctgattgg
     5821 ctatgcgttt atttccgcac tgctgccagt gtggctgatc ctcgcaccgc gcgactatct
     5881 ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa
     5941 cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc cgctgtggaa
     6001 aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat ccggcttcca
     6061 cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg acgcgcgttt
     6121 catcggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc tggttgctgc
     6181 gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg gcctcggcat
     6241 caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca tggcacagct
     6301 gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc
     6361 agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg
     6421 tggtgcacca acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc
     6481 tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca tcctgaccgc
     6541 gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg gtaacttcat
     6601 cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt
     6661 gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag
     6721 cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc tggtactggg
     6781 caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc
     6841 tgtatggctg cttatctgca ccacctgggc gctgggcctg aaactgttca gcaccaaccc
     6901 gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac
     6961 tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca actacaccaa
     7021 cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa
     7081 aacctggctg aaggtgcgta acagcgacaa acgtactgac aaagaaacac cgtacgttcc
     7141 aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccggttgcct tatccggctt
     7201 acaaaatgtc tatcgtaggc cggataaggc gtttacgccg catccggcaa atccccagcc
     7261 cggaccttcc gggctttacg catatctgga aggcaaaatg tttggtaact taggacaggc
     7321 aaaaaaatat ctcggccagg cggcgaagat gttgattggc attccagact acgacaacta
     7381 cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg aagaattctt
     7441 ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta
     7501 atggagagag catgaacccg attgcagtta ccctactcac cggtttttta ggcgcaggaa
     7561 aaaccaccct gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg
     7621 aaaacgaatt cggcgaagtc tccgttgatg atcaattgat tggcgatcgc gccacgcaga
     7681 tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaagacgccc
     7741 tactcgacct gctggataat ctcgacaagg gcaatattca gttcgaccgt ctggtgattg
     7801 aatgcaccgg catggccgat cccggcccga ttattcagac ctttttctcc catgagattt
     7861 tatgccagcg ttacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg
     7921 agcagatgaa ccagttcacc atcgcccagt cacaggttgg ctacgccgac cgtattctgc
     7981 tgacgaaaac cgacgtcgca ggcgaagcag aaaaactgcg tgaacgcctg gcgcgcatca
     8041 acgcccgtgc accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca
     8101 acaccaacgg ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt ttccatttta
     8161 tcgcggataa acaaaacgat atttcgtcga ttgtggtgga actggattac ccggtagata
     8221 tcagcgaagt ttcccgcgtg atggaaaacc tgctgctgga gtcggcggat aaactactgc
     8281 gttacaaagg gatgctatgg attgacggcg aacctaaccg cttgctgttc cagggcgtcc
     8341 aacgcctcta cagcgccgac tgggaccgcc cgtggggcga tgaaaaaccg catagtacga
     8401 tggtgtttat cgggattcag ttgccggaag atgagattcg ggcagcgttt gcggggttga
     8461 ggaagtaatt ttggatgatg gccgccatgt ggcggcctga attatttttt gttctgatcg
     8521 cgcagcatcg cttcacgtaa aattgcgttc aggcgagttt gataaccttt acctggtcgc
     8581 ttcagccatt ccatcacatc agcatcaata cgcaccgaag cctgagtttt caatggccga
     8641 taaaacttac cgcgttctgc gttagaccat tgctcatcgc tggaaggtgg gatgtcgctg
     8701 taatcaatat cctcgtcgga tttgttcgcc agcgccttca gttcggcctc atgctgcaca
     8761 tcgagtgtag agggtgtccc tcgtttatgt ttaaccatgc tcatagcggc tcctctcttt
     8821 acgatctgct tttcgtgcac tgatgattcg aatgttggaa atagcatcac gttttaggaa
     8881 aagataggga agttgatatg aaggggctgg atggcagcat tgggacacca tccagcactt
     8941 attcaaacct gatagcaatc caccaaaaat gccatcattt cactggcatc tttgctgtga
     9001 taaaagcgca ccattttgct gttgaactcc agcgcacgag ctgcgggtac actaatggga
     9061 ttgtaaccgt ggcgcattaa ccagccgttc atcatatggc gactggtacg cttgttgcca
     9121 tcaaagaaaa actgaaaata agcaccaaat aaaaacattg ccagcgcccc ttcgaacggt
     9181 ggcatctctt ttatttgtgc cacacctgcg gcgaatattc gattcagttc aggtgcacct
     9241 gcttcagtgg ctggtggaaa atgagtgccc agttcaccga gatgaacgcg cgcattgtaa
     9301 tgtaactctt caccttcgcc gcgaaaaacg ccccactcca gcgcctcatt acgcgcaata
     9361 atgccgttaa gtaacgtgaa cgttttctta tcaaggtcga aacgcccgaa ttgcacaagt
     9421 tcaatcagtt tttttgcgct atctgccagg ttcaacacct gctcagtatc actgacctta
     9481 tgcccgccaa cagtgacacc atcaagcaac gttttaactt caacaaaggt ataggggtta
     9541 ttttccagtt gcgcggcatc ccagacaaac cctgccatat gcgttttgaa ggtaaacaag
     9601 gctgcgcgcg taccgacaat ttgcatcgcc gggatggcat ttgcattcca gctcaggtcc
     9661 agtagtttgg ttgtcattag aggatccttg attgcatggt aaagaggata ttattccata
     9721 ttaagccatc gcgttatcca tctgcttttc aaatgcctga cgaattaatc ccatcacata
     9781 aggaagctca gaaagatcgc tgaaacctac ttcaacatcg ccattgcccc aacggcctat
     9841 atctgtgaca tctttagcca tacattttgg gtcaaccagt tcatagaatg gcatatttag
     9901 tgataaacgt aagcgacttt tttgcggcac cacatcaaca aaattcgtct ccgctttgaa
     9961 ggcaacatag agcttcaata tttctcgcgt aaccccagcg tccagcgtca taacggcatc
    10021 agaaaggctg tcaaaaagta cgcggatgtg cgtaccttct tcaagttgcg ggaaatcagt
    10081 aaggacatat cccgttcctc gttcaacgac ggtgcgataa cgagccaaca cgtcttccga
    10141 aagtttgggg atcgcccaga cgtctattgc cagctcggcc agacgttccg ctctcttttg
    10201 tatcgctgca cgatcccata tttcacattg ccctaacccc gtgttcaggc gtaatggact
    10261 ttccttaaag ccacctttta tatctcgctt cttcgcaaat ggacggtcgc tgtagcttga
    10321 gttatagcca gttaacgtta aattacccaa cgtatggagc cactttttat ggatacgctt
    10381 ccattccgga ccaaggtcat cgcgccactt cgccgataaa ttagggttct gcggcatgat
    10441 atgttcaatc gtataagtgt caacatagac ccgttcttta cgatcgtggt tttcaaatct
    10501 gcgtagccaa tagctgcgat tacggaagtt gtagagatcg cgagttttca gttcacgttg
    10561 aaattcatca tcatccggga aacgacggta ggaaggcagt tccagcaaat gcgccgcaat
    10621 gctctccaga taacgctctt tatccagcac tttgtaaaac gtggcgaaag ttttagtaaa
    10681 tgagttggtg ggaatggcac agactgcgcg ccggaagata tacgactcaa ttaaacgcgt
    10741 ggcacttaaa aaatccgcgg cagaaagaat tccttgttta tagtcgtgat aaagcaccaa
    10801 caggaacggc caggcggcat cgactttcag ctcacgtaga tcctgaaacg ccatctttaa
    10861 tattttgtct ttttcgctat ccagcgccat cgcacaaaaa taatccgcat aggtacgaat
    10921 atccgcaacc agcgcctcca tcccgctatc catcactcct ggttttcgcg catagcgttt
    10981 aaaggcttca tagacatcac caattcgcgg aatctcacct gtttttaccg tcaggtaatt
    11041 acgcataaaa ctgtcgaaat agtcagtgta tcccttctga ccaaatgcca gctccatggg
    11101 ccgccagtaa tcatcgtaca gttggctttg atgttctggc tccagcccca tcagaacata
    11161 gttgcgaacc agatccgcct gactcagcgc ttttcctgtt gagttcatgc tttcaaagat
    11221 tagttgcgga ttatcctgac cgcgattcaa ggcgacatcc acaatcagca gtttggaaag
    11281 gccctgacat aacgcggtga aattgccgtg gagtttgcct atttgctcat ggaaaaactc
    11341 aaaattctct ttcacgcgta gagagcaatt ttctgccagc ggtttttgtc taatcaatgc
    11401 cagcaggctc tctctgtccg tttgcgtaag cagtaactta aaaccacgct cgcccgattc
    11461 atacggattg agcagataat agtttcgtaa tttcatggcg gagaaaccat ccatcacctc
    11521 gtcatcgtca aggtgtctgg acaacgcttc cagcaataac atcgccgttg tcaggcgttg
    11581 ctgaccgtca atcaccagta agggagagat ccccgaaacc tgatataggc cctgttcgat
    11641 atacaccacc gagccgataa aatgcgccgg gatatcatca cgctgcccgg cacgtattac
    11701 atcgtcccag agctggcggc actgttcctc tgaccatgaa taagtacgct gataaatggg
    11761 aataacgaat tgctgagagc gttttagaaa atcgagtaat cgggcttcgg tggctttcat
    11821 gaatgactcc tgtcaaattg tgctggcaaa cgacgtcatt atgctgcgaa taaaaaataa
    11881 aagttagcgt ctttgtttct gcaaaaatac cattccagga taaatcaaaa tatattaaga
    11941 aacacctcag aacgcattca ttagaacaat taacacttcc aatgtatatt tataacgcat
    12001 tgaaaatgat catgtttttg atcatcacaa cgatagtttg atataacaaa gcattcactt
    12061 cacattgaat atctccctct ttttcagggc aaggaatgct ttttatgtca tcaactaatt
    12121 tcagtcagat cgctgccttc ctgtggtctg tcgccgacct gttacgcggc gattttaaac
    12181 aatcacaata tggacgaatt attcttcctt ttaccctttt gcgtcgtctg gaatgcgtac
    12241 tcgaaacaac gaaagacgcg gtgattaccg aggcgaaaaa agtaaaagcc atgaaactgc
    12301 cggaggaagc ccaggagaaa atgatcctgc gggcgactaa tggcctcacg ttcttcaatg
    12361 cctcagcgat ggatctcagc aaaatggggc aaaatggcat acaggacaac ctggaaaact
    12421 acatccagtc gttctcttcg gatgcccgcg aaattttcga acacttcaag ttcagcgagt
    12481 ttgtcgggca actggctgat gcgaacttgc tgttcaaagt ggtgcaaata ttcgctaaag
    12541 cagatttata cccagagcac gttaccaacc acgatatggg gctggtattt gaggagctga
    12601 tccgacgttt tgccgagagt tctaacgaaa ccgccgggga gcactttacc ccgcgtgata
    12661 tcgtcaatct gaccacctcg ctggtgtttt tcgatgatga tgacgcgctc aataaagacg
    12721 gcatcatccg caccatttac gacccaacag cgggaaccgg cggctttctc tcctccggca
    12781 tggagttcgt ccaccaacaa aaccccaatg ccgtgatgcg cgcgtttggt caggagttaa
    12841 acccggaatc ctacgccatc tgcaaagccg acatgctgat taagggccag gatgtcagcc
    12901 ttatcaaact cgggaacacc ctgtccaacg atcagctccc cgcagaaaaa ttcgattaca
    12961 tgctttctaa tccacctttc ggcgtggact ggaaaaagat agaaaccgac atcaacgacg
    13021 agcacaaact caaaggtgct gatggtcgct ttggccccgg cctaccccgc gtctccgacg
    13081 gctcgttgct gtttctgctg cacctgatca gcaaaatgcg cgatgccaaa agcggtggcg
    13141 ggcggatcgg catcattctt aatggttcgc cgttattcac cggcggtgca ggaagcggtg
    13201 aaagcgagat ccgccgctac attctggaag cggatttgct ggaaggcatc atcgccctgc
    13261 ccaccgacat gttttacaac accggtatcg ctacctatgt gtggattttg tcgaataaaa
    13321 aagcgccaga acgcaaaggc aaagtccagc ttatcgacgg cagcaacctg tgcggcaaga
    13381 tgcgtaaatc gctggggtca aaacgtaata ttctcgggga ggaggatatt ggtttaatca
    13441 cccgcacgtt tggcgatttc gaacccgttg ccaccactac gcttgcggcg ttaggcctgg
    13501 agaaagcacc cgaacaaaaa tccagccggg gacgccagcc cgcaacaacc aaaaccgagg
    13561 cggcaaaaac ttttgccagc aaagtcttcc acagcaccga ctttggctat cgtcgcatca
    13621 ccgttgagcg cccgttgcgc ctgtcggctc agataagtga tgacgccatt gccacattgc
    13681 gctttgcgcc gaagccgttt aacgctccga tggcgcaact atatgacgcc tttgctttcc
    13741 agtggcagga cggtaattac ggcgacttaa cagcggtaga aagtgaagcc cgcgccattc
    13801 ttaaagccga ttttagcgag ctgaaagaga agcaaattaa agatctgctc gacagcaaat
    13861 tatggctggc ccagcgcggg ttaatggaaa aagcgcggcg tattcaggcg gcgatgggta
    13921 tgcaggcggg cggcaaagac accgttagca atgactttaa tcagttccag ttgacgctga
    13981 aagaagcgct cagaaccgca ggcgtgaagc tggatgcgaa agagaacaag caatttattg
    14041 atgccattac ccgcaaaaac ccggacgccg agccggtagt cagcaaggtc ctgaaagagg
    14101 cggcccaacc gctgtatggc gcgtgtgagt acaacggaaa ggttgtggag tttgagccgg
    14161 atggcgacct gcgtgataac gagaatgtgc cgttaaatcc ggcggtatct accagcgagt
    14221 tgatcgaggg ttacttcaaa gcagaagtat tgccgcatgt tgcggatgcg tggattaacg
    14281 ccgataagcg cgatgcgaaa gatggggata tcgggattgt ggggtatgaa attccgttta
    14341 atcgtcattt ttatgtttat cagccgccaa ggccgctgga agaaattgat gcggacctgg
    14401 atgcggtaag cgctgagatt atgaaactgt tgcaggaggt acattcctga tggcagggtt
    14461 aaataaatat caggcgtatc cggagtatag ggattctggg atggagtggt gtaatgaatt
    14521 accactaaac tggaaaaaaa ctaagctgag gtggctatcc aatatttttg caggaggaac
    14581 tccctccaaa aatgttattg attattggga gaatggtaca gttccctgga ttagttctgg
    14641 agcagtaaat caaggttata tagtcgagcc atccacatat atatctaacg ctgcacttga
    14701 aaatagtagt gccaagtgga taccaaaagg ggcacttgtt gtcgctttag ctgggcaggg
    14761 aaaaaccaaa ggtatggttg cacaacttgg tattaatacc acttgtaacc aatccatggc
    14821 ggccattgta ttgtataaaa agaatcaatc tcgctatata ttttggtggc tcatttctaa
    14881 ttatcagaat atacgtaaca tggctggcgg tgatttacga gacggtttaa accttgaact
    14941 tttaggcgat atacaatgcc caaaacctcg taatgatgaa tcttctaaga ttgcactttt
    15001 ccttgatcat gaaaccgcaa aaatcgataa cctaatcgag aaacaacaac aacttattga
    15061 actactgaaa gaaaaacgtc aggccgtgat tagccatgca gtgaccaaag ggctaaatcc
    15121 tgatgtgccg atgaaagatt ctggggttga gtggttgggt gaagttccga aacactggca
    15181 catatgcaag ttaaaatggt ttgctaattt gaaaagtggt gattttataa ccagcaattc
    15241 aatagaacca gaaggaaact atcctgtata tggtggaaat ggattaagag gatattattc
    15301 ttattttaca cacaatggtg aatatgttct tattggtaga caaggtgctc tttgcggaaa
    15361 tataaattac gcaattggaa agttttgggc ttcagaacat gctgttgttg tcaccccaaa
    15421 cgaaagagct gttacaatat ggttaggaga attattaaga ataatgaact taaaccaata
    15481 ttcagtttct gctgcacaac cggggcttgc ggttgaaaga attactgatt tatatattcc
    15541 aatcccacca tatcaagagc aagtaaatat cggaacttat atatctaaat atatttctct
    15601 agataaaaaa ttgattgagc atagtacaga taatatcgaa ctccttaaag aacgccgcac
    15661 cgcccttatc tccgccgccg tcaccggcaa aatcgacctg cgcaactgga ccgcccccat
    15721 ctccgaagcg gaaacaccca cggaggccag cgcatgagcc acgacatcac aaaagaggtc
    15781 aacctacaaa acgatatcat cgcacagatg gtggaaaaag gctggattgt cggcaaaggt
    15841 gacgcctacg acagagagcg tgcactatac gcacaagaca ccttaaactt cgtacaaacc
    15901 acccaaccgc aggagtggga gaaattttcc cgcgtctacc ccaacgacac cgagcgccat
    15961 ttcctcgacg cgctggtcgc gcagcttaaa aaagccgaca gcaacgcaac cgatatccag
    16021 tcgcgcacct ggggtacgct cggcgtactt cgtcacggcc tgaaaatccg caatgcccgt
    16081 ttttcgctgt gtcagtttaa accggaacat aatctcaacc cggaaacgct ggcgcgctat
    16141 cagcagaata tctgccgtat tgtgccggaa ctggtctaca gcccgtatgc ctcaaatagc
    16201 gtctttgcag aaacgggcgt gaaagccaaa aaatggcgca tcgacctggt gctgtttatc
    16261 aacggcctgc caattgccac gctggagctg aaatccgaat tcaaacaggc ggtgcagaac
    16321 gccattgcgc agtacaaaaa gacccgcctg ccgaaagatc ccgccaccaa taaaccggaa
    16381 ccgctgctga cctttaagcg cggcgcgctg gtgcattttg ccgtcagcca gtatgacgta
    16441 tttatgacca ccaaactcgc gggtgacagc accttcttcc tgccgtttaa caaaggcacc
    16501 aaagacggag gcgcaggcaa cgagatcccc gaagataccc accgctacgc caccgattat
    16561 ctgtggaatg aagtgctgct gccggacaac ttattaaaaa tcctcgccag ttttgtccat
    16621 ttgcagattg aagaaaaaga ggagtggaac gggatgaaag cgaagaaaga gagcctgatt
    16681 ttcccgcgtt atcatcagtg ggatgtggtc aataaactgc tggaggccgc gacgattgaa
    16741 ggaaccggcc ataaatacct gatccagcac agcgccggtt ccggaaagtc gaactctatt
    16801 gcctggaccg cccaccagct ttctcgtctg tacgacgtga acggggaaaa acagttccac
    16861 tccgttatcg tggtgacgga ccgcacggtg ctggacgacc agcttcagga caccatctgg
    16921 cagttcgagc acgccgacgg cgtggtcggg cgcatcaata acagagaggg cgacggctcc
    16981 aaatcggaaa agctcgccaa cgcgctggaa aaatcgcagc cgattattat cgtcaccatc
    17041 cagacgttcc cgttcgtatt gaaagcaatc gaaaacagcg tcagcctgaa acagcgtaaa
    17101 tacgcggtaa tcgccgacga agcgcactcc tcgcagagcg gttctaccgc ccgtcagctt
    17161 aaagaagtgc tgatgagcga agaaatggat gatgacttgg tactgtcgtc agaagatatc
    17221 ctggatgcga ccattgccgc caggaaaggc agcagtaacc tgaactacta cgcctttacc
    17281 gccacgccaa aatccaaaac cctggaactc tttggtcgct gtccgcgtcc cgatgaaccg
    17341 gcctcaaaaa ccaataaacc tgaggccttc cacgtctact ccatgcgcca ggcaatcgaa
    17401 gaaggcttta tcctcgacgt gctgaaaaac tacaccaact acaaagtggc gtatcagttg
    17461 ctgcaaaagt tggaagataa agaccgcgaa gtcgatagca aacgggcgaa ggtaaaactc
    17521 aatcagtggg tgacgctgca cgaccacaat atctcgcaga aggtgaaggt gattgtggag
    17581 cacttccgca aacatgtgat gcacctgctt ggcggccagg ccaaagcgat ggtggtcacc
    17641 agttcacgca aagccgctgt gcgctacaag ctggcgtttg ataaatacat tagcgagaac
    17701 aattaccaga aaatcagcgc gatggtggcg ttttccgggg aagttgaatt ccaggaaggc
    17761 gaccctaaca gcctggcatt gttaaaccag aaatttactg aaataaacat gaaccccggc
    17821 ctgaaagggc gggatatgcg caaagccttc gacagcgatg attatcaggt catgctggtt
    17881 gccaataaat tccagaccgg ttttgaccag ccgaagctgt gcgcgatgta cgtggataaa
    17941 gcccttggcg gcgtcgagtg cgtgcagacg ctctcccgcc tgaatcgcac ctatccgggc
    18001 aaagcggaat caggcacttt tgtcctcgat ttttataacg atccagacga gatcctcggc
    18061 gcattccagc cctatttcca gacggcggaa ctgaccgatg tcagcgatcc gcagcaggta
    18121 ttcgatctgt ttgaaaaact gcgcgccacg cagatttttc agtggcatga agtcgagcag
    18181 ttctgcgagg cgtacttcat caaaaataaa tcgaatgcgg cgatcagtaa tatctgtaaa
    18241 ccggcggtcg aacgctggaa aaagcgttat ctgatggcaa tagacgccta tttgaccgcc
    18301 aaagagatgt tcgagcgcac taaaaaaacg ggcgatacgg tgttaatcac caatgcggaa
    18361 aacagtttta aagcctgtaa gcaggagaaa gatcgtctgg agattttcaa gaaagatctc
    18421 ggcagctttg ttcgctttta tgagtttatg tcgcaaatcg ttgagtacga tgacaaggat
    18481 ctggaaaagc taagcctgtt tgcgcgccat ttacgcccac tgctgcatga gcaacgtctg
    18541 gaagaggatg aaattgatct cagtaatgtg gagatgagtc actatcgttt atcgaaaatc
    18601 catgagcaag atttacgctt acaggaagaa acgcctgact atacgctgga tgcaagtaat
    18661 aatattggta cggcaaaagc gaaagataaa aaagaggagt ttttatctca ggttctttct
    18721 cggatgaatg aattattcat tagtgaccat ttgaccgaca aagatatgat cagcaatgct
    18781 tttgcggtgt ataacaaaat aactgaaaat gaaaatgtta tgacacaaat taaaaacaat
    18841 ccgccagaac aagcgattct tggtgatttc ctccaggcag ttgataatgc tgtaatggac
    18901 agtaatgacg ctcggcaaga atttatgtt
//
LOCUS       DAJOTB010000091        18639 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJOTB010000091 DAJOTB010000000
VERSION     DAJOTB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.091"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(657..1979)
                     /gene="hipA"
                     /locus_tag="OHN28_003115"
     CDS             complement(657..1979)
                     /gene="hipA"
                     /locus_tag="OHN28_003115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCQ2298465.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK"
     gene            complement(1979..2245)
                     /gene="hipB"
                     /locus_tag="OHN28_003116"
     CDS             complement(1979..2245)
                     /gene="hipB"
                     /locus_tag="OHN28_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCQ2298466.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            2553..3311
                     /gene="tam"
                     /locus_tag="OHN28_003117"
     CDS             2553..3311
                     /gene="tam"
                     /locus_tag="OHN28_003117"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCQ2298467.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3315..4229)
                     /locus_tag="OHN28_003118"
     CDS             complement(3315..4229)
                     /locus_tag="OHN28_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCQ2298468.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4426..5877)
                     /gene="uxaB"
                     /locus_tag="OHN28_003119"
     CDS             complement(4426..5877)
                     /gene="uxaB"
                     /locus_tag="OHN28_003119"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCQ2298469.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(6104..7522)
                     /locus_tag="OHN28_003120"
     CDS             complement(6104..7522)
                     /locus_tag="OHN28_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2298470.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7661..8020)
                     /locus_tag="OHN28_003121"
     CDS             complement(7661..8020)
                     /locus_tag="OHN28_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCQ2298471.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(8020..8946)
                     /gene="glsB"
                     /locus_tag="OHN28_003122"
     CDS             complement(8020..8946)
                     /gene="glsB"
                     /locus_tag="OHN28_003122"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCQ2298472.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(9010..10398)
                     /gene="sad"
                     /locus_tag="OHN28_003123"
     CDS             complement(9010..10398)
                     /gene="sad"
                     /locus_tag="OHN28_003123"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2298473.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10499..11380
                     /locus_tag="OHN28_003124"
     CDS             10499..11380
                     /locus_tag="OHN28_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2298474.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            11458..11541
                     /gene="yneK"
                     /locus_tag="OHN28_003125"
                     /pseudo
     CDS             11458..11541
                     /gene="yneK"
                     /locus_tag="OHN28_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11719..12909
                     /gene="ydeA"
                     /locus_tag="OHN28_003126"
     CDS             11719..12909
                     /gene="ydeA"
                     /locus_tag="OHN28_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCQ2298475.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12934..13599)
                     /gene="marC"
                     /locus_tag="OHN28_003127"
     CDS             complement(12934..13599)
                     /gene="marC"
                     /locus_tag="OHN28_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCQ2298476.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13811..14245
                     /gene="marR"
                     /locus_tag="OHN28_003128"
     CDS             13811..14245
                     /gene="marR"
                     /locus_tag="OHN28_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCQ2298477.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            14266..14649
                     /gene="marA"
                     /locus_tag="OHN28_003129"
     CDS             14266..14649
                     /gene="marA"
                     /locus_tag="OHN28_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCQ2298478.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14681..14899
                     /gene="marB"
                     /locus_tag="OHN28_003130"
     CDS             14681..14899
                     /gene="marB"
                     /locus_tag="OHN28_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCQ2298479.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14930..15829)
                     /gene="eamA"
                     /locus_tag="OHN28_003131"
     CDS             complement(14930..15829)
                     /gene="eamA"
                     /locus_tag="OHN28_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCQ2298480.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            16024..17211
                     /gene="ydeE"
                     /locus_tag="OHN28_003132"
     CDS             16024..17211
                     /gene="ydeE"
                     /locus_tag="OHN28_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCQ2298481.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            17338..17433
                     /gene="mgtS"
                     /locus_tag="OHN28_003133"
     CDS             17338..17433
                     /gene="mgtS"
                     /locus_tag="OHN28_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCQ2298482.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17652..18542)
                     /gene="dgcZ"
                     /locus_tag="OHN28_003134"
     CDS             complement(17652..18542)
                     /gene="dgcZ"
                     /locus_tag="OHN28_003134"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCQ2298483.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         4872 a         4693 c         4306 g         4768 t
ORIGIN      
        1 ttcattatgc atgttattta taaacagcga ctaaacagaa tattattgcg aacaagggaa
       61 gtgtcaggaa aggtcaagag gtactaatag tttatatggc tataaacatt agctatggtt
      121 aatgtttgcc tggtgaaact aactgaatcc catgaataat gcagtaatta gtagtgcttt
      181 catatctgat acctgtttta aacgatatat ttggaagttg acgaatatcg gaaatagctt
      241 cctccatcag ctgtagtcac tgttctctgt atttcacatt gcatatcaac acgatatgct
      301 gagattagct aacttgagat taaagataat tacacggcgc tatgaattat attcagaagc
      361 gtgagggata tagtgccatc aaagcgctgg ctgctgtaaa caacgcaatt atccagctca
      421 tggtctatgg tgtgccatca ctgaacagag gttgattttt ctaccgaaac gggagggaag
      481 tcagaaatca caacgtattg atttatctgt gtgtggatgt gaatggtgaa gtaaaggacc
      541 ttcataatca atttaacata atatacatta tgcgcaccaa tataaaccaa gggcgaatcc
      601 agtcgttggc ggttcatgat tgttctgctc attaacaacg accaaaaccc catatcttac
      661 ttaataccgt attctcggct taaccgatcg tgcaacctca acacattggt ttcaactgcc
      721 gtcaccacgt tctccggaaa atctgtcggt aatgaagtct tcacgttatc cagtgctgcc
      781 ggaatcattc tggcaaagtc actcaggatt tcatgcatct gcacttccgg gaatttcagc
      841 acctttgctg tcgccagaaa atgtcgcgga taaattttat cgattgccgt ttttttgcct
      901 ttggatgcgt taagccccat tgccagtttg agatcgctga tgtgtattcc cgtaccgcca
      961 aggaccggaa atgccgaaat gatgtcgtaa aatggtgtga gccgataact gccgccagcc
     1021 tgaataaata cggagaagtt ttttgcatgg ccatccgttg cgccaatcaa ccactggaag
     1081 acctgaaact tcataaaatc atagcgatct ttcagcgcct cgctggaccc catcaaaaaa
     1141 gccataattt gcgcgatacc ggggcctcca tctgattcat atttcaccga tgaaggtaaa
     1201 ccgaatgtct ggcacatatc ctcctgtggc aagcgaagta aaaccgttcg ctcagtattc
     1261 caacgcctgt caaaacgttc gaccgctaac gcgcgcaccc ttcccgcttt aatgatttct
     1321 gcgtccggaa cattcaaccc gagttctttc gccagcagca aacagtaata ctcattatca
     1381 acgctttggc taagatcgag cgtcgcattg ggctgtctga tttcgccaat cggcaatttg
     1441 ataatgtgcg tcgttggcgt tattcccttc ggaatgcacc agtcattgcc tattctgagc
     1501 agcgcagtct tctcctgtgc gccagcaacc gagatgcgaa agtcattttc ttctctaatc
     1561 atgcctaacg ggatatctgc tttataagcc gttaatactt cttcaagtct ggcttcagta
     1621 agcttttccc atgccattat cggacacgtt acggtttcat cttcggggat taacgtcacg
     1681 gcaccaacgc tgtctcgccc tatttctgac aataaatcaa acggttgtct ggatttagca
     1741 tgataacgtt taacgatccg gtcacgtaca atcgggctat cgggtaacag gttatcgaag
     1801 aagttaaata cggcatcaga ggtgatattc cccctctgca atggcagcga aagtgacaac
     1861 ggtctggcat aacggctggc taaccactca gctgcatact taaaggtgtg cgcgccgttg
     1921 gctaacttcg ttaactcgcc tacccgctgg ttgttcatcc aagtgactag tttaggcatt
     1981 accactccag atcttgctgt tctgttgctt ctggcgaggc atttttcgcg tcgcatagcg
     2041 tcattgagag ttcaagcgac tgtaaaatct taaaaaatgt cgtgagcgag gtattgtcag
     2101 gattgttttc gaaattggaa attgtcgcct gcttaatgcc gatttttttc gccaactcgc
     2161 tctgcgtcca gccgttttgc tggcgaacca gtttcattgc attcgccaat tgcgttgggc
     2221 tatagatctt ctgaaagctc atcatgtcat acgtccacag caaatttatc cgcttaaggg
     2281 gatattatca gttttatcct ttagtgagga taaagtcaat ctggaaacac atcttatccg
     2341 ctctacggga taaacgctgc gatatccgcg atcgcggata aaaaggagga tttaaaaaag
     2401 cacatcctaa tgcggtggct tctttgacta ccggaatctg taatcttaat gaccgggccg
     2461 cgtaaaaaac gtctgttcaa tggggtgatg ccgtgaggcg aatttatcag ttttatctac
     2521 aattggggta acgcgctgac gggagtaaaa aaatgtctga ctggaacccc tctttatatc
     2581 tgcactttgc cgctgaacga tcgcgtccgg cggtggagct gcttgccagg gtgccgctgg
     2641 agaatatcga atacattgcc gatcttggct gtggcccagg taacatcacc gcccttctac
     2701 accaacgttg gcctgctgcc aggataacgg gcatcgactc atctccggcg atgattgctg
     2761 aagcgcgcag tgctttgcca gactgcctgt ttgtggaagc agatatccgc aactggcaac
     2821 cggaacaggc actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact
     2881 acgaattgtt tcctcatttg gtttcgttac ttaattcgca tggtgtgttg gcaatacaga
     2941 tgccagataa ctggctagag ccaacccatg tgctcatgcg cgaagtcgcc tgggaacaaa
     3001 attatccgga tcgcgggcgt gagccgctgg ctggcgttca tgcttactac gatattctga
     3061 gtgaagccgg atgtgaggtc gatatctggc gaaccactta ctatcaccag atgccgtcgc
     3121 accaggcgat tatcgattgg gtgaccgcca ctggattacg tccgtggtta caggatctga
     3181 ccgagagcga acagcagcat tttcttacgc gctaccatca gatgctggaa gagcaatatc
     3241 ccctccagga gaacggacag atattgctgg catttccgcg tctgtttatt gttgcccgcc
     3301 gtacggagta attatcacgt cagctggtaa tggcgatcgg gaagaatttt cgctggaatt
     3361 tctgcttcat cattcatctg taacaggtca atttcaatag cgttgcagat ggcatccagc
     3421 ggtaaatcat tgttttcagt accgaaggga tcttccagtt cttccgccag acaatccagc
     3481 gaaataaaag tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg
     3541 accagcgcaa acggcaacat gatacaaaac agatacacgg tgcgatgcaa aatcagggtg
     3601 taggcaaagg gaattggcgt ataggcaatg cgctcgcatc ctgccaggac cgctgaaata
     3661 tcattaagcc gatcgttgag gctaataaac aggatatctg aaagttgtcc attgcgtcgc
     3721 tgaaccgcca accactcccc cattattaac aagatgcggt tagctggaga gttagaagcc
     3781 agtacacgct gaagatcttc agtcttgaga taatgagcca gcacttctgc ctgtggctgt
     3841 ttgcgtaatg tcatgcgtaa acagtgggcg aagacgattt gcagccgggc aaactccctt
     3901 acacttgccg aatccggtaa tgtcgttttt acctcgcgca gtaacgaccg cgaggcaatc
     3961 atcaactgcc cccaaagttt tcgcgcttca acgtaacggg cgtaaccggc attattacga
     4021 aaaccaagaa aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaattta
     4081 atgcccaaat gcgtgtacca gggcagcatg aaaataacag cgatcgaaaa gagaaaattg
     4141 agtagtaagc gcgaggatat cttggataat actgaaccgt gccagacaaa aatacggcgc
     4201 agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtggaaa agtcgcccta
     4261 ttaaacgtga ttatgatcac attctcaaga tactacttac aaattaccta caagattaca
     4321 acattaacag gtgctgcatg tggcacctgt taatgataaa ggcatataga atgttgtaac
     4381 taatatagcg gaataagaaa cccggtcgaa accgggttca gagtattagc acaacgggcg
     4441 tacagcttcg cgcatccctt tttcgagaat cgcatccagg tcattagcaa cctgctcgac
     4501 cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca acacaatcgc
     4561 taccagttcc tgggtcccga taacacggtc acgatactgg ctccagagtt gctgataacg
     4621 ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccattacgca caccgcgata
     4681 gaacgcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc cgtttgcctt
     4741 ctgccctgcc agcagctgcg gcaggatgcg tgtgcggaac ttggtcatac cgttgagcgc
     4801 gatagacagc agttgatgct tgatgtacgg gttacggaaa cgtccggtga ctgcactggc
     4861 gaaagactcc agctcatcgc gtggcaaatc cagtaccgga ataatttctt cgtagatggc
     4921 tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg tatccagccc
     4981 tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg ttgaggattg ccactttgcg
     5041 ttctttatac ggtttaatat cgtcaacgat cagcacgtta agcggatatt tgtccagacg
     5101 aagttcagtc gctaaggatt tcggcccctg aatcacaaac aggtaaaagt gttcagcggt
     5161 gtcgaggaaa ccatcgtggt agcccagttc ttcttccagt ttagccactt catcacgcgg
     5221 ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct gatccagcca
     5281 ttgaataaat gcttcaggca atgcccactc ttgcgcatag cgcagcacca gttcacgcaa
     5341 cgcgtcgcca ttatagtcaa tcaactcaca cgggatgatg atccaacctt tatccagcgc
     5401 accgttgaaa tggctaaaac gttcgaacag cagacgggtc agttttgccg gataacttac
     5461 cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg atacccgctt cggtggtgtt
     5521 agagaaaaca aagcgcattt ccgggttgtg agccagtttc aggaattcat cgtattcact
     5581 atagacgctg atttcacgat taaccgagcg aatcagacgc gcgtcgctga ccgcttcacc
     5641 cttctcattc aggccacgga taatggtggt atacagacca tcctgcgtgc tcagtgacgg
     5701 cgggaatgaa gtttcaatcg gacgaacaac gaccacgcca gaattcagat cggtgtgctc
     5761 attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt caccaaactg
     5821 aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg ttttcacaat
     5881 gggttccctt ctgattagtc atacaacctg tttgaattgg tacgacaggt tagcaaactt
     5941 taatacgtcg aacccctgtt ttgatcaact cctgatgatt aatgagcagt tttatgagaa
     6001 aagtgtggcg cggatcatgg tttaatcgag gaaacaggcc ttttcttgga tcataaagtg
     6061 gtagaacaca ttgcatttaa ttcgctctga gtaaataaag atgtcagaca acttcctcac
     6121 cgtaacgcat agtgctggta cggttgcgcc catctttctt cgagcgatac agacacgtat
     6181 cggcttcgac catcaattta ttaaagtcat cggtcagcgt tcggtacgat gcgcaaccac
     6241 taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg aacggttgca
     6301 gttcaacgcc tttacggatt ttttccgcca ttagcaggcc atcgacagga ttcacagacg
     6361 gcactgcaac agcaaattct tcgccgccca ttcgcgccac cagcccctta tcaccgacaa
     6421 tcttctgaat atgctgggca aacacgctta acactttatc gccacattca tgtccatagt
     6481 tatcgttaat gcttttgaaa tagtcgatat caagcagcat gaccgtcaga tgttgtgtct
     6541 gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa acctgggtca
     6601 gaaaatcaaa atcggctcgc agcgcaactt gcttcattag cgaattgatc gctgccacgc
     6661 taaaggaaac cataattggg catatcgcca tcgtggcaat accgagacgt gcggagaaca
     6721 tttgcggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt gccaccagta
     6781 cgacttccac agcgccggtg acaaaggtca acagacatgt tacttgcggc gtatagcgca
     6841 ctgcacacca gattaatgca gggagcggaa acgccagact ccccgctccg ccaatgacta
     6901 ccgaggcaat aaccgacaca ataagcgcga tagcaggcat catctgctct gctttaaagc
     6961 gcggcagcac tccaggaatt gccaacgtca gcatacaagg cacgatcaac acgcccgttg
     7021 agaattgctc actaaaccag tcggcaagca aaggccagaa atccagagta tcaatactga
     7081 ccgaaccaat cgccccgaca atagcgcata ataatgcggc aagcagacag taattaaata
     7141 accgtaaggc acttactggc tcatacttat tttttcccag acgcttatcc cgcgcgacca
     7201 gtaaggcgac ggtaacaata aacatcatat tggagaaatt gatagccagt gaaaccagcc
     7261 cccattcggt ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat
     7321 gcagtcgatt aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc
     7381 agaaaagaga aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg
     7441 ttagcacaaa aatagcgatg ctattacgta gcagatgacc ttcctggaac aaacggaaag
     7501 ttgaaatggg ctgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct
     7561 caacgcaata agtaaattct tagaaaggat agcatgttcg ctgacaacgg cggcaactaa
     7621 agatacctaa tgaatgccat tttatcgatt tccgtaatta tcatggctga ttcatctgta
     7681 taaccagcca gtgatagatt accgtcacaa tataacgttg ttgctcctca cttaacgctt
     7741 ccccctgagg aatgttatgc cacttagcca gacaaccgcg acaacaggtc gcggtggcgt
     7801 gttgagcgat aaaaaccgga tgcccgcgca tgggggtttg cttaccgtcg ttagccggta
     7861 atgccggagc aagacgttta gcgacaaaat ctgccgcatg ttgttcaatg actggcgcgc
     7921 ctttctccag acaatactga cgctctttca cacccaaacg aaagcgagag cgaaattttg
     7981 aacgggataa acgcgcgaag agtggatcga gagactgcat taataaactg aacgccctaa
     8041 ttgtttcgtc aattgttcaa ggacggcaat gcccgcaagc gagttaccgg catcatccag
     8101 ttccggactc cagacagcga tggccatttc atgcggaaca atcgccacaa taccgccacc
     8161 aacgccagat tttgccggta gtcccacccg ccaggcaaac tcccccgcgt tctggtacat
     8221 accgctggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc
     8281 atcaatatga atagctttcc cctgattcgc cagaaagaca aacgtccggg ccagctctac
     8341 acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacgtcatg
     8401 atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc
     8461 aaattcggaa cgcgctacca ccgtatcgta ggaaatatca gacacaccac ttaagccgcg
     8521 cacgacttcc agcatacgtt gccgaggtgc gcttaatcgc ccttgcaaca tatcgcagac
     8581 caccagcgca ccggcattaa taaacggatt acgcggaata ccctgctcca tttccagttg
     8641 cactaaagaa ttgaatggtg atccagacgg atctttgccg acgcgttgcc agatttcctc
     8701 ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga
     8761 aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat
     8821 ccccaatcgg gaaccgtcta ccgtagccag cgccggaata taatccgcga ctttaccctg
     8881 accaatgagc ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac
     8941 tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa
     9001 tatatagcgt cagacccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc
     9061 gaaatgggaa agctcacgac caaagccact cttcttcacg ccaccaaagg cgactcgcgc
     9121 atcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg
     9181 tctggcctgt gtttcgtcgg tggtgaaaat ggtcgctgaa aggccgaact cactatcatt
     9241 agccagttcc agcgcatgtt ctgcatcttt cgcaatggtg attgccgcaa cggggccaaa
     9301 catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata
     9361 gtagttacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag
     9421 tgttttttcc acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag
     9481 agcgttgtct tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg
     9541 ttcggtaaat gccgaagcaa ttccctcttc gatgataaag cgttttgccg ctgcacaaac
     9601 ctgtccggta ttctgataac gtccggctac cgccgctttc accgccagtt ccagatcggc
     9661 atcgttaagc acaataaacg gatccgaacc acccagttcc agtacgcatt ttttcagtgc
     9721 cgctccagcc tgtgcgccaa tagccgctcc agcacgaaca cttccggtca ctgtgacagc
     9781 agcaatgcgc gaatctttaa tcatctgact gacaccgtcg ttgtcggcat tcagccagcc
     9841 atatacgcct tgtgggatac ccgcatcttt aaacacctgg gcaacgagct gtgcacagcc
     9901 catcacattc ggcgcatgtt taagtaagta gccgttacca gcaagaatga tgggaacagc
     9961 gccacgcatc acctgccata acggaaaatt ccacggcata atcgccagaa tcgtccccaa
    10021 cggtcgatac tcaataaccg cctgctgatt ttccaccagc gtaggttctg ccttcagcat
    10081 tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg
    10141 cgcctggttg attggtttgc ccatttcacg ggtgatcatt tgcgccattt cttcgctacg
    10201 ggcgcgcaga gccttaccga tatcacgcag tttttcagca cgataatcta tatttgtctc
    10261 gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat cgtcaacacc
    10321 agcccacggc agcacagaaa gttgttcacc ggtggcagga tttatcgaaa ttgcatgagt
    10381 tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga
    10441 tttactcttg ctttaaaatg aataatatta agccacttat tcacgaatcg agaatgccat
    10501 ggatctgacc caactggaga tgttcaacgc ggttgccgaa gctggcagca ttacccaggc
    10561 tgcagcaaaa gtgcatcgcg tgccgtcgaa tctcactacc cgtctacgtc agctggaaac
    10621 agaactgggg gttgatctgt ttattcgcga gaatcagcgt ttacgtctct cgcctgccgg
    10681 gcataacttt ttacgctata gccaacaaat tctcgcatta gtggatgaag cgcggagcgt
    10741 tgtcgctggc gatgagccgc aaggtttatt ttctctggga tcgctggaaa gcaccgctgc
    10801 agtgcgcatt ccggccacgc tggcggaatt taaccgtcgt tatcccaaaa ttcagttttc
    10861 actttccacc ggcccttccg gcactatgct ggaaggtgtg ctggagggaa aactgaatgc
    10921 ggcgtttatt gatggaccca ttaaccatac tgccatcgac gggataccgg tataccgcga
    10981 ggaactgatg atcgtcacgc cacaaggaca tgcgccagta acccgtgcca gtcaggtgaa
    11041 tggcagtaac atttatgcct tccgcgccaa ttgttcgtat cgtcgccact tcgaaagctg
    11101 gtttcatgct gacggtgccg ctccgggaac tattcacgag atggagtctt atcacgggat
    11161 gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta tgctggaaag
    11221 tatgccgggg catcaccagg ttgaatcgtg gccgttagct gagcaatggc gttggttaac
    11281 aacctggctg gtctggcgtc gtggtgcgaa aacgcgtccg cttgaggcat ttattcaact
    11341 gctggatgcg cctgactcgg caaaacaggg ttaccaatga gctatttttg atagttcatg
    11401 cggctaatat gctctatata gtgatgttcc aatgacttat gactatatgg ggcaaatatg
    11461 gttacgccag taagcatcag caattacata tctcttcctg atgattttcc cgttcgtaat
    11521 attgcacctc aagtaaaaga agaacaggcc cccttcctga tcgtcattaa aatgtgatgt
    11581 taacaactct ctgatgcgtg acagaacaac cgtcagagag atggaaatgc gatccagatc
    11641 acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa
    11701 ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtagc gtggctacgg
    11761 gtcgttacgc tggcagtcgc cgcctttatc ttcaatacca ccgaatttgt ccctgttggc
    11821 ctgctctctg acattgcgca tagttttcac atgcaaaccg ctcaggtcgg catcatgttg
    11881 acaatttatg catgggtagt ggcgctaatg tcattgcctt ttatgttaat gaccagccag
    11941 gttgaacgcc gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg
    12001 tcgtttttat cgtggagttt taccgttctg gtgatcagtc gcattggtgt ggcttttgca
    12061 catgcgattt tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag
    12121 cgagcacagg cattgagttt aattgccacc ggtacagcac tggcaatggt attaggttta
    12181 cctctcgggc gcattgtggg gcagtatttt ggctggcgaa tgaccttctt cgcgattggt
    12241 atcggggcgc ttcttaccct tttgtgcctg attaagttac ttcccttgct gcccagtgag
    12301 cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt
    12361 tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct
    12421 tttgtacaaa acattgcggg attcagcgcc aactttgcca cggcattact gttattactc
    12481 ggtggtgcgg gcattattgg cagcgtgatt ttcggtaaac tgggtaatca gtatgcgtct
    12541 gcgttggtga gtacagcgat tgcgctattg ctggtgtgcc tggcactgct gctacctgcg
    12601 gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc
    12661 atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgccac cgacgtcgcg
    12721 atggcgctat tctccgggat atttaatatt ggaatcggcg cgggtgcgtt ggtaggtaat
    12781 caggtgagtc tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt
    12841 gctgcgttaa tatggtcaat catcattttt cgccgctggc cagtgacact cgaagaacag
    12901 acgcaatagt tgaaaggccc attcgggcct tttttaatga tacgttttaa tgatttccag
    12961 gatgccgtta ataataaact gcacgcccat acataccagc aggaatccca tcagacggga
    13021 gatcgcttca ataccaccct tgcccaccag ccgcataatt gcgccagagc tgcgtagact
    13081 tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca
    13141 atcagcgaag gttgaactct gacgcactgt ggaggccgag ctaataatca tcgcaatggt
    13201 tcccggaccg gcggtacttg gcattgccag tggtacaaaa gcaatattgg cactgggttc
    13261 atcttccagc tcttccgact tgcttttcac ttccggtgaa tcaatcgctt tctgttgcgg
    13321 aaagagcatc cgaaaaccga taaacgcgac gatcaaaccg cctgcaattc gcagaccggg
    13381 aatcgaaatg ccaaatgtat ccatcaccag ttgcccggcg taatacgcca ccatcatgat
    13441 ggcaaatacg tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt
    13501 gcctgccagg ccaagaaata acgcgacagt tgttaatggg ttagctaacg gcagtaacac
    13561 caccagcccc aggcctattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta
    13621 tctggtttat cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta
    13681 accttgcatc gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt
    13741 cattcatttg acttatactt gcctgggcaa tattatcccc tgcaactaat tacttgccag
    13801 ggcaactaat gtgaaaagta ccagcgatct gttcaatgaa attattccat tgggtcgttt
    13861 aatccatatg gttaatcaga agaaagatcg cctgcttaac gagtatctgt ctccgctgga
    13921 tattaccgcg gcacagttta aggtgctctg ctctatccgc tgcgcggcgt gtattactcc
    13981 ggttgaactg aaaaaagtgt tgtcggtcga cctgggggca ctgacccgta tgctggatcg
    14041 cctggtctgt aaaggctggg tggaaaggtt gccgaacccg aatgataagc gcggcgtact
    14101 ggtaaaactt accaccagcg gcgcggcaat atgtgaacaa tgccatcaat tagttggcca
    14161 ggacctgcat caagaattaa caaaaaacct gacggcggac gaagtggcaa cacttgagca
    14221 tttgcttaag aaagtcctgc cgtaaacgaa aaaagaggta tgacgatgtc cagacgcaat
    14281 actgacgcta ttaccattca tagcattttg gactggatcg aggacaacct ggaatcgcca
    14341 ctgtcactgg agaaagtgtc agagcgttcg ggttactcca aatggcacct gcaacggatg
    14401 tttaaaaaag aaaccggtca ttcattaggc caatacatcc gtagccgtaa gatgacggaa
    14461 atcgcgcaaa agctgaagga aagtaacgag ccgatactct atctggcaga acgatatggc
    14521 ttcgagtccc aacaaactct gacccgaacc ttcaaaaatt actttgatgt tccgccgcat
    14581 aaataccgga tgaccaatat gcagggtgaa tcgcgctttt tacatccatt aaatcattac
    14641 aacaactagt tgataacgtg acaacgtcac tgaggcaatc atgaaaccac ttttatccgc
    14701 aatagcaact gcgcttattc tcttttctgc gcagggcgtt gcggaacaaa ccacgcagcc
    14761 ggttgttact tcctgtggca atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt
    14821 tgatttaaat cacatgggta ctggcagtga taagtcggat gcgctcggcg tgccctatta
    14881 taatcaacac gctatgtagt ttgttctggc cccgacatct cggggcttat taacttccca
    14941 cttttaccgc tttacgccac cgcaagccaa atacattgat atacagcccg gtcataatga
    15001 gcaccgcacc gaaaaattgc agacccgtta agcgttcatc cagcaatagt gccgcacttg
    15061 ccagtccaac tacaggcacc agtaacgata acggtgcaac ccgccaggtt tcatagcgtc
    15121 caagcagtgt tccccagatc ccataaccaa caattgtcgc tacaaatgcc agatacatca
    15181 gagacaagat ggtggtcata tcgatagtaa ccagactgtg aatcatggtt gcggaaccat
    15241 cgagaatcag cgaggcagca aagaagggaa tgattgggat taatgcgctc cagattacca
    15301 gcgacatcac cgccggacgc gttgcgtgcg acatgatctt tttattgaag atgttgccac
    15361 acgcccaact aaatgctgcc gccagggtca acataaagcc gagcatcgcc acatgctgac
    15421 cgttcagact atcttcgatt aacaccagta cgccaaaaat cgctaaggcg atccccgtca
    15481 attgtttgcc atgcagtcgc tcaccgaaag taaacgcgcc aagcacgata gtaaaaaacg
    15541 cctgtgcctg taacaccagc gaagccagtc cagcaggcat accaaagtta atggcgcaga
    15601 acagaaaagc aaactgcgca aaactgatgg ttaatccata ccccagcagc aaattcagtg
    15661 gtactttcgg tcgtgcgaca aaaaagatgg ccgggaaagc aaccagcata aagcgcaaac
    15721 cggccagcat cagcggtggc atgttatgaa gccccacttt gatgaccaca aaatttagcc
    15781 cccatacaac cactaccagt agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc
    15841 tgaatttcat cttttgtaag caatcaactt agctgaattt acttttcttt aacagttgat
    15901 tcgttagtca ccgtttacga cggcattaat gcgcaaataa gtcgctatac ttcggatttt
    15961 tgccatgcta ttcctttaca tctctaaaac aaaacataac gaaacgcact gccggacaga
    16021 caaatgaact tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta
    16081 accatcggac gcggcgctac gctgccgttt atgaccattt acttgagtcg ccagtacagc
    16141 ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt
    16201 agcctcggtt ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca
    16261 attaccgcct tcgctagcgg ttttattgcc attcctttag tgaataacgt gacgctggtg
    16321 gtgctctttt ttgcccttat taactgcgcc tactccgttt ttgctaccgt gctgaaagcc
    16381 tggtttgccg acaatctttc gtccaccagc aaaacgaaga ttttctcaat caactacacc
    16441 atgctcaaca ttggctggac catcggtccg ccactcggca cgctgttggt gatgcagagc
    16501 atcaatctgc ccttctggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa
    16561 atttgggtaa agcgcagcga gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg
    16621 aaagttttat tacaagataa agcactgttg tggtttacct gctctggctt tctggcttct
    16681 tttgttagcg gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggt
    16741 gattttgccg aaaaggtggt cgcggttgtt cttccggtga atgctgccat ggtagttacg
    16801 ttgcaatatt ccgtaggccg ccgccttaac ccggctaaca tccgcgcgct gatgacagca
    16861 ggcaccttct gtttcgtcat cgggctggtc ggttttattt tttccggcaa cagcctgcta
    16921 atgtggggta tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag
    16981 tatatgttga ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttccgcccag
    17041 tctttaggct ggcttggtgc tgcgattaac ccattagtga gtggcatagt acttaccagt
    17101 ctgccgcctt tctcgctgtt tatcatctta gcgttggtga tcgttgttgc gtgggtgctg
    17161 atgttaaaag gaattcgtgc aagaccgtgg gggcagcccg cgctttgttg atttaagtcg
    17221 aacacaataa gaatttaatt cagccttcgt ttaggttacc tctgctaata tctttctcat
    17281 tgagatgaaa attaaggtaa gcgaggaaac acaccacacc ataaacggag gcaaataatg
    17341 ctgggtaata tgaatgtttt tatggccgta ctgggaataa ttttattttc tgggtttctg
    17401 gccgcgtatt tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc
    17461 ggccccttgt tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc
    17521 cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag
    17581 gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg
    17641 ttaaacggag cttaaactcg gttaatcaca ttttgttcgt caataaacat gcagcgattc
    17701 cttccggttt gcttaccctc atacattgcc ctgtccgctc ttccaatgac cacatccaga
    17761 ggctcttcag gaaatgcacg actcacacca gcagtcacgg taatgttgat atgcccttca
    17821 gaatgtgtga tggcatggtt atcgactaac tggcaaattc tgacacctgc acgacatgct
    17881 tcttcatcat tagtcgcttt gacaataatg ataaattctt cgcccccgta gcgataaacc
    17941 gtttcgtaat cgcgcgtcca actggctaag taagttgcga gggtgcgtaa tactacatcg
    18001 ccgattaaat gcccgtaggt atcattaacc aatttaaatc ggtctatatc caacaacatt
    18061 aaataaagat tcagaggttc agcattgcgt aactgatgat cgaaggattc atcaagaacc
    18121 cgacgacccg gcaatcccgt cagaacatcc atattgctac ggaccgtaag caaataaatt
    18181 ttgtaatcgg ttaatgccgc agtaaaagaa agcaaccctt cctgaaaagc gtcgaaatgc
    18241 gcgtcctgcc agtgattttc aacaatagcc agcattaatt cccgaccaca gttatgcata
    18301 tgttgatggg cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggcccc
    18361 agatgatcaa tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct
    18421 ggcttactgg catctctcgc gaccacgctg tgaaacatac ttaccagcca ctggtagtgg
    18481 gcatcgatag ccttattgag atttaacaag atggcatcaa tttccgttgt cttcttgatc
    18541 attgccactc ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat
    18601 tacgtcaata ttttcataga aatccgaagt tatgagcat
//
LOCUS       DAJOTB010000092        18545 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJOTB010000092 DAJOTB010000000
VERSION     DAJOTB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.092"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            75..1214
                     /gene="arnB"
                     /locus_tag="OHN28_003135"
     CDS             75..1214
                     /gene="arnB"
                     /locus_tag="OHN28_003135"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCQ2298484.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1218..2186
                     /gene="arnC"
                     /locus_tag="OHN28_003136"
     CDS             1218..2186
                     /gene="arnC"
                     /locus_tag="OHN28_003136"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCQ2298485.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2186..4168
                     /gene="arnA"
                     /locus_tag="OHN28_003137"
     CDS             2186..4168
                     /gene="arnA"
                     /locus_tag="OHN28_003137"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCQ2298486.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4165..5055
                     /locus_tag="OHN28_003138"
     CDS             4165..5055
                     /locus_tag="OHN28_003138"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCQ2298487.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5055..6707
                     /gene="arnT"
                     /locus_tag="OHN28_003139"
     CDS             5055..6707
                     /gene="arnT"
                     /locus_tag="OHN28_003139"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCQ2298488.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            6704..7039
                     /gene="arnE"
                     /locus_tag="OHN28_003140"
     CDS             6704..7039
                     /gene="arnE"
                     /locus_tag="OHN28_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCQ2298489.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7039..7425
                     /gene="arnF"
                     /locus_tag="OHN28_003141"
     CDS             7039..7425
                     /gene="arnF"
                     /locus_tag="OHN28_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCQ2298490.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="OHN28_003142"
     CDS             complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="OHN28_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCQ2298491.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7795..9150)
                     /gene="menE"
                     /locus_tag="OHN28_003143"
     CDS             complement(7795..9150)
                     /gene="menE"
                     /locus_tag="OHN28_003143"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCQ2298492.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9147..10109)
                     /gene="menC"
                     /locus_tag="OHN28_003144"
     CDS             complement(9147..10109)
                     /gene="menC"
                     /locus_tag="OHN28_003144"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCQ2298493.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10109..10966)
                     /gene="menB"
                     /locus_tag="OHN28_003145"
     CDS             complement(10109..10966)
                     /gene="menB"
                     /locus_tag="OHN28_003145"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCQ2298494.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10981..11739)
                     /gene="menH"
                     /locus_tag="OHN28_003146"
     CDS             complement(10981..11739)
                     /gene="menH"
                     /locus_tag="OHN28_003146"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCQ2298495.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11736..13406)
                     /gene="menD"
                     /locus_tag="OHN28_003147"
     CDS             complement(11736..13406)
                     /gene="menD"
                     /locus_tag="OHN28_003147"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCQ2298496.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERGRFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13495..14790)
                     /gene="menF"
                     /locus_tag="OHN28_003148"
     CDS             complement(13495..14790)
                     /gene="menF"
                     /locus_tag="OHN28_003148"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCQ2298497.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="OHN28_003149"
     CDS             complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="OHN28_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCQ2298498.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15229..15690)
                     /locus_tag="OHN28_003150"
     CDS             complement(15229..15690)
                     /locus_tag="OHN28_003150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2298499.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15755..16672
                     /gene="rbn"
                     /locus_tag="OHN28_003151"
     CDS             15755..16672
                     /gene="rbn"
                     /locus_tag="OHN28_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCQ2298500.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16793..18535)
                     /locus_tag="OHN28_003152"
     CDS             complement(16793..18535)
                     /locus_tag="OHN28_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2298501.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPATARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKH
                     ITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     ASAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
BASE COUNT         4283 a         4990 c         4689 g         4583 t
ORIGIN      
        1 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatga
       61 cggatggaaa aacaatgtca ggatttttgc ctttttcgcg accagcaatg ggcgtagagg
      121 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      181 aggcgcttga acaagctttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      241 cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag ggcgatgaag
      301 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg ctgggtgcaa
      361 cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gcgatcgaag
      421 cagccattac accacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      481 atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      541 cccatgccgt aggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt
      601 tttcgtttca cgccatcaaa aatattacct gtgcggaagg tggcctgatt gttactgata
      661 atgaaaatct tgcccgccag ctgcggatgc tgaaatttca cggtctgggt gtcgatgcct
      721 atgacagaca tacctggggc cgtgcaccgc aggctgaagt cttaacgccg ggctataagt
      781 acaatctgac cgatattaac gccgcgattg ccctgacaca gttagtcaaa ttagagcacc
      841 tcaacacccg gcggcgcgaa attgcccagc aatatcagca agcactggca actctcccct
      901 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
      961 gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa
     1021 gaggcattgg taccgggtta catttccgcg ctgctcacac acaaaaatat tatcgcgagc
     1081 gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttcattgc
     1141 cgctgttccc ggatatgacc accgccgatg ccgaccgcgt catcacagcc cttcagcaac
     1201 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1261 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc gcagcctgtg
     1321 aatcactggg gaaagagtat gaaatcctgc tgattgatga cggcagtagc gataattccg
     1381 cgcatgtgct ggtcgaagcc tcacaagcgg agggcagcca tattgtgtct attttgctta
     1441 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtc accggcgact
     1501 taattattac cctcgatgcc gatctccaga atccgccaga ggagatcccc cgtctggtgg
     1561 caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct
     1621 ggtttcgtaa aaccgcttcg aagatgatta accggcttat tcagcgcacc accggcaaag
     1681 cgatgggtga ctatggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1741 tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1801 ccattgaaat tccagtgcac catgccgagc gtgagtttgg tgaatccaaa tacagcttta
     1861 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1921 tgttgagttt gctcggcagc attattgcga ttggcggttt tagcattgcg gtgctgctgg
     1981 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2041 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattgctc ggtgaatata
     2101 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2161 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2221 atgcctcggt attgaagccc tgctgtctgc cggttacgaa attagcgcca tttttaccca
     2281 tactgataac cccggtgaaa aagcctttta tggttcggtt gcccgactgg cagcggaaag
     2341 aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg aacgcattgc
     2401 ccaactatca ccagaggtga ttttctcttt ttattatcgc catcttattt gcgatgagat
     2461 tttgcagctc gctccgagag gtgcatttaa tctgcatggt tcgctgttac caaaatatcg
     2521 tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaactg gcgttacatt
     2581 gcaccgaatg gtgaaacgtg ccgatgccgg ggccattgtg gcacaactgc gcgttgccat
     2641 tgcgccagac gatatcgcca ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2701 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
     2761 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttcctcg aatggcataa
     2821 gccggcatcc gtactgcaca acatggtgcg tgccgttgcc gatccgtggc cgggtgcctt
     2881 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     2941 caaagcactg ccggggagcg tgatttctgt tgcaccactg ctgattgcct gtggcgatgg
     3001 cgcgctggaa atcgtcactg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
     3061 agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgtgccgc
     3121 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3181 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
     3241 gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata tcagtattca
     3301 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtgttgccgc tggtggcgat
     3361 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg atttcgaaga
     3421 gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcgac
     3481 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggatcatt ctaatttaat
     3541 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tctaaacaat tacttgatcg
     3601 ggtgatctgg gcctatggcg aaaaagaagg tttacagttc accctcttcc gcccgtttaa
     3661 ctggatgggg ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
     3721 tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa
     3781 acaaaaacgc tgctttactg atattcggga tggtatcgag gcgttatacc gcattatcga
     3841 aaatgcggga aatcgctgcg atggcgaaat tatcaacatt ggcaatcctg agaacgaagc
     3901 gagcattgaa gaactgggtg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     3961 ttacttccca ccgtttgcgg gctttcgtgt tgtcgaaagt agcagctact atggcaaagg
     4021 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct gcctggactg
     4081 ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4141 cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga tgtcgatacc
     4201 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
     4261 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4321 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
     4381 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4441 atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4501 tggcaagccc atagcggtaa ttgggatcgg caaacaatga tcgacgatat tgcacgcggc
     4561 cttcgcaccc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgctggctgg
     4621 cgtgctgacc aacaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4681 tgtcgtgggg cgatgccgtt tcgtccattg ctcgaatcag gaacccccgg cactgcgcaa
     4741 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4801 tttaacggtt ggttactcaa ccgcatccag cgagataaag gcacgccggt ttataccatt
     4861 catgccgaag ttgaaggctg cgcttatcag cataattttg tggatctcct gaaacgcgca
     4921 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagggac gttgccgctc
     4981 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatg
     5041 gcgggtagtc actgatgaaa tcggtacgtt accttatcgg cctcttcgca tttattgcct
     5101 gctattacct gttaccgatc agcacgcgtc tgctctggca accagatgaa acgcgttatg
     5161 cggaaatcag tcgggaaatg ctggcatccg gcgactggat tgttccccat ctgttagggc
     5221 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
     5281 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5341 cgctgatgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ttactcgcca
     5401 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5461 cgtttattgc cttctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5521 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5581 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5641 caacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
     5701 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
     5761 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagcgc
     5821 cgttctggta ctacttgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
     5881 ccggtgcact gtacgctggc tggaaaaacc gcaagcattc cgcaaccgtt tatttgttga
     5941 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
     6001 ttctttcctg ctttgcacct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa
     6061 aaaataatcc tctggcgttg cggattaatg gctggattaa catcgctttt ggcgtcactg
     6121 gcattattgc cacatttgtg gtctccccct ggggaccaat gaacacaccg gtgtggcaaa
     6181 ccttcgagag ctataaagtc ttttgtgcct ggtctatttt ttcgctatgg gcatttttcg
     6241 gctggtacac cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg
     6301 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
     6361 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6421 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
     6481 gccagacagg tgagttgaaa tatggcctta attatccgga tgcaaaaggg agatttgtca
     6541 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
     6601 tgctttcggt tgaccgcgat gaagatatca atagtctcgc cattccgccc gctgatgcca
     6661 tcgatcgtca gggacgtctg gtactcattc agtatcggcc caaatgatct ggctaacatt
     6721 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6781 tgcggcggta aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6841 tcttggcctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
     6901 ctacccgatg ttaagtctga attttgtctg ggtaacgctg gctgcagtaa aactgtggca
     6961 cgagccggta tcgctgcgtc actggtgtgg ggtggcgttc attattggcg gcattgtgat
     7021 cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat tatcgcctcc
     7081 gctgcgcaat taagcttggg ttttgcggcg agtcatctgc cgccaatgac gcacttatgg
     7141 gattttatcg cggcgctgtt agcttttggc ctggatgccc ggatattgtt actcgggctg
     7201 ctgggttatc tgctttccgt tttttgttgg tataaaacac tacataaact tgcgctaagc
     7261 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7321 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7381 gggttgatgc tgattttcct gcccacaaca aaacaacgtt actgagtttt ccctgccgct
     7441 ttacactgcc gttcccactc atcggggcta taacagcttg cagaaagaac tttcaccgtg
     7501 tgcagctttt cacgattaat acagtaaagc gcattctgca aaggcgagag taaatccccc
     7561 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7621 cagagcataa gtacatgtct gttattttgt ttattgcagc acgatttttt gaccagccat
     7681 tccattgcat tatcctgttt gataagagtt ttcacatcaa tttacagatt aacagataaa
     7741 cctattatca acggcaatgg aatggcaaaa ttgcgaagtg aaactaatga tttcttaatc
     7801 ctggcgttgc acccactctt ttaacgcctg acgtgaaatt ttaataccgc cgtttttcag
     7861 ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgggccagct tatctttcac
     7921 ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg gtcgatgacc
     7981 aaactccttg tcagcaacgg gaacgataaa cacctgcaaa accgcaggat gtgcagcaat
     8041 aacgcgctcg acttcttctg gctgaatgcc ttcgccacca ctgaagaaca gattgtctaa
     8101 acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg tggcgtacca
     8161 gccttcgtca ttaaccagtg gaacccgttg cccgttacgc cagtaacctt ctgccatgct
     8221 ggcagcccgc agccacactt cgtcgtttac gattttcact tcccgacccg gcagcggcga
     8281 accgacgtct gccagaccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag
     8341 accatagccg caaaagcacc gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg
     8401 gatagccgcg ccgccaagta aaaccgcttt cagggaaacg gaactacggt taaccagcaa
     8461 acgccagagt tgtgttggca ccagtgaagc gtgcgtacag cctgccagca tttgctccaa
     8521 tggctgttta tcacgtaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc
     8581 ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg
     8641 aatcagcgat aacacccctt cggcgctggc aagatgagcc tgataagtat gaacagcggc
     8701 tttcggcaag ccagtggaac cagaggttaa cgtcattgag cacagacgcg tcggctgcca
     8761 cgccacggca tgtacgcctt caacctgctg aatatgcagc gacgttaacg ccggaaacgt
     8821 gttttcccct tccggcacca gcgcaaattg cagcgtaaga ttgggcagca attcctcaag
     8881 cagcggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc
     8941 cagccaggcc agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgcccgc
     9001 aaccacaccc tgcgccgcaa agctggaggc taattcatcg acgcgagcac aaagctcgcg
     9061 ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca
     9121 gtgacgccac ggccagtcag agaagatcat aacagccgct ccagtgcatc aacgtccact
     9181 aacggcagtg gactccccgg ccagcgacga acctgctgca cctgcatcag atccagcgtg
     9241 tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag ttgcattaga
     9301 cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc atgcgccgcc
     9361 ttcacctgct cgcgtacttt ctccaggctg cccgtgagcg tgggtttgat aactaccgcg
     9421 cgcacgccct cttcagctac aaaggcaaaa tccggctcgc gcaagctttc atcccaggca
     9481 atggcaatgc cggtttcacg ggcaaacgca cgcgaatcat cgcgggtttt gcacggctct
     9541 tcgagaaatg cgatgcggtg gcgataatcc gggttaacgt atttggcaaa ctgctggcct
     9601 ttcagcggtg tccaggcgcg attcgcgtca agacgcaaat gcagatccgg aattgcctcc
     9661 agcaacagat tcaccaccat gccgtcgcgc acggcttcat acaatcccac tttgaccttc
     9721 gccactttct cgccaggcat atcggcaagt ttgaggatca gatcgtcagg atcgccatta
     9781 cacagcggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat
     9841 gcacaactta cgccaaaggc aacggaaggc atctgcggta attcgcaatc gcctgccagc
     9901 cagttattta cccaggcaag cagcacactt tgcgcatctt cccaggtttc ctgactgaag
     9961 cccggcagtg gggagatctc cccccaccct tcgcgctcac cctcacgcag gcaaacatac
    10021 agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc
    10081 tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag
    10141 gctgacgttt ctggttgaaa gcattgcgac cttcctgacc ttcttccgtc atgtagaaca
    10201 gcatggtagc gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca
    10261 gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttca cggcaccaac
    10321 ggacggtttc tttttccaga tccgccagcg gtaccacggt gtttacaagg cccatatcca
    10381 gcgcctgttt cgcgtcgtac tgacggcaca agaaccagat ttcacgcgct tttttctgtc
    10441 cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc
    10501 cagtctggcc gaagatggca ttatcagccg cgatagtcag gtcgcacatc atgtgcagaa
    10561 cgtgaccgcc gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac
    10621 ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca tctttatagc
    10681 cgccgtaatc gccacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcac
    10741 cagtcagaat gatcacgccg atgttgtcgt cataacgcgc atccgccagc gcctggatca
    10801 tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt
    10861 ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaaaccttcg gagcagtcgt
    10921 gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag
    10981 tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt cccggtgcgc
    11041 gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagagcgcg
    11101 gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt
    11161 agcacgtaaa tcgggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc
    11221 gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc
    11281 aaaaacaggc tgttgatacc aatcggcaaa taccgccgtt aacggttctg tacgaaaacg
    11341 ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat tttgcaaccc
    11401 aggatgcccg ccttcgacaa caactccaca aagccccgcc agctcctggc aagccgccat
    11461 catcgccacc cgtccgccaa gcgagtaccc caccagccag aagttaagga tgttgtaact
    11521 aaccagcgtt ttacacagta agtcggtgac atcatcaaat ccatcgacgc tgatcgtcgc
    11581 tgaaccaccg tgacctggga gatcaacata caaccgcgag tagtcggcaa acgcctcgcc
    11641 cacttcttgc cattcatggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa
    11701 acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga
    11761 agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg
    11821 gtcggcgtac gccaggcatc gactagtgtc gtttcaagtt cctgccagtt ttgcggacga
    11881 tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga
    11941 tagaaacgcc cgcgctcgct tttcggcgtt ggcaacagcg aaaaaatttg cccgccgttg
    12001 ttgttcacca caattaatac cagcggtgcg gaaacctggc gcaataacgc cagcgcgttg
    12061 agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc
    12121 tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggcaccacg gttgctgtac
    12181 accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgcaccac caggctgtta
    12241 ccgacaaaca attgcccctg ttcaggcaaa tagtcgctga tgcgatgcgc cagttgcgct
    12301 tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc caggcgcggg
    12361 atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata
    12421 ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca
    12481 acaatccagt actcttctgg ttcacagctt gcctgccatt ggaggagtcg tttccccgtc
    12541 aggctgcttc ccaattgcac cactatttgt gcctgttgca gttcgctggt cgctttggca
    12601 ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag cacgtcgcca
    12661 atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc ttccgcactc
    12721 atgcgcccgg caaccaccac accgcgcttt tgtcgccaga agaaccagtc gcgctgtttt
    12781 tcactttccc gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcgccc
    12841 agccgctgtt gccagctaat ccccgtgtcg tccatttcgc catacagcgg ttcagcaaac
    12901 gggcagttga tatggactcc accagcatga agcgtaccga gagcgtggtc gatggtagaa
    12961 accagccaac gtgcggggat atcctgggtc gggcgcggca gtgaaatact gtgcgtgggg
    13021 tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc aatcagctcc
    13081 ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc ttcaatcagc
    13141 gccggataga gatttgctac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg
    13201 ctgactttcg ccagccccag cgccagatgc cccagtccac gctcatcgaa atgggtgtgg
    13261 tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagccaggg
    13321 gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag
    13381 cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat
    13441 aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc
    13501 catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc
    13561 ggggtcggaa ccacggacaa tgcctgcgcc agcatatagc cgcacgatat tgccgctgat
    13621 ttttgctgag cgcagggaaa cgcagaattc actttgctgt aatgaaagat agcccgcaga
    13681 accggcgtac cattctcgag tgaacggttc gtgacgggcg ataaactgtc gcgccagatc
    13741 gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc
    13801 cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgtactttac gtagacgcag
    13861 tacctgcggc ggtaaaacat ccagcgtccg ggtatcggcc tgcaatcgtt ggcagatatc
    13921 ttccaccacc aacatgttct cgcgctggtt tttatcatcc gccatcagcc actcacctaa
    13981 ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg cttcggtacg
    14041 cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gaaccaagaa aagcattttc
    14101 gccatcaaag gccatgtaaa aatggtagca attcagattg agccgacgac tggcagccat
    14161 catcgctgcc gcattgaccg gacttgcgaa atgcaggtca gttgcccgag caagcacaac
    14221 tttgtcgagc tcgccttcgg cgatggtttt cgttgccagt tcgattaata gcgtccagcc
    14281 cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaatccag gcaggggctt
    14341 gatactcacc agtgtggcga taaattcttt tgccttgatt gcatcgtgct gaagggagct
    14401 ttcgctgaat agcgtcaacc gcagcgtggc tttaccgcca caacgtcgcc attccaggcg
    14461 tggtaaaagt aaatagccct gcgacgggtc aaacgcattc aacccccaga tgcgtaagtc
    14521 ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt
    14581 aatcgcaccc aggacggcag cttcttcatc accattacgt tgttgccagt agaattgcgg
    14641 atacacctgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg
    14701 aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag
    14761 attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat
    14821 tatacggggt acgacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc
    14881 cagcaacagt cctagaacca gcccaacggc cgcacccaca ccaattcctt gccagggttt
    14941 ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc
    15001 tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag
    15061 ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc
    15121 actaagcagc gtcaggtcgt catcaatacg tgtatcacca aactgattag acattctcgt
    15181 tctccatttg tgtaaaacct gtcactaact atagacaagg gttattgatt acgcctgaat
    15241 tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcataaatat cggtcacagg
    15301 gatgaaacca aattgcccat aaaagttttg taaatgcgcc tgcgccccca gataaatcgg
    15361 cttctccggc cagcggcgag tacacgattc cagcgcctta ctcatcaatt gctgaccgat
    15421 tttttcaccg cgtaaggctt cgctgacaat cacccgacca atcacaacgg gttgcagttc
    15481 gtcgtcactt ttcagaatcc tcgcatacgc caccagtgtc ccgttatgcc aaccgagaat
    15541 gtggcgattt tctccctcca gatcgtcacc atcgatatcc tggtaaggac agttttgttc
    15601 cactacaaat accgcacaac gcagttgtaa taaggcataa agctgagaaa cggataaatc
    15661 agaatgatgc agatcttgcc agtcgatcat ggaaggctcc tttgtgctgt ttgctttata
    15721 ctagccggaa tattttcagc agcgtgatga actcatggaa ttaatttttt taggtacttc
    15781 agccggtgtg ccaacccgta cgcgtaatgt cacggcaata ttgcttaacc tgcaacatcc
    15841 gacccagagc ggactttggt tatttgactg cggtgaaggc acccagcatc agctactgca
    15901 tactgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca
    15961 tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt
    16021 aacgatttat ggtccgcacg gtatccgtga atttgtggag accgcgctgc ggattagcgg
    16081 ctcatggacc gattatccgc tggaaattgt cgaaattagc actggcgaaa ttttcgatga
    16141 tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt atggttatcg
    16201 tattgaagaa catgataaac caggtgcatt aaatgcccag gcattaaaag ctgctggcgt
    16261 gccgcctggc ccgctgtttc aggaattaaa agcgggcaaa acaatcatgc tggatgatgg
    16321 aaggcagatt aatggcgcag attacttagc cgctccagtg ccgggtaaag cgctcgctat
    16381 tttcggcgat accggcccct gcgatgccgc gctggagctt gctaaaggcg tcgacgtaat
    16441 ggttcacgaa gcgactctgg atatggcgat ggaggccaaa gccaacagtc gcggccatag
    16501 ttctacacgc caggcggcgg cactagcccg tgaggctgga gtcggtaagc taatcattac
    16561 ccacgtcagc tcacgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgtcggtc
    16621 aattttcccg gcgactgaac tggcgaatga tttcgcggtg tttagcattt aagtcgaaag
    16681 cggtggttag ttagcagatt aatttaacga taccaacaca tttatttgac attttttcat
    16741 ctgtttgccg gatacgtttg atgtcgatat caggcaaaca gaattatcat ttttagtttt
    16801 gtgaattgtc cagatctttc gccagtccaa tcaggcgaat aaattccgct ctgtaaccct
    16861 gtggatcttc cccttttgcc ttctgagccc actgtttgat ctgctgccag gaggtattgt
    16921 tcaggtattc agaaccgcgt aacttttgcc catatgcagc cactgctgcg cgaaagcgca
    16981 tatcttctga aggtgccttt attgcagatg ccaacgggaa ttcaactaac tgactttctt
    17041 ttccctgtgg ggatttccaa cgaatcttta accaggcaag ttcttttgtt ttgtccgatt
    17101 tcgctgattt gttatccggg gcatagcgta acttatcaat tgatgctttt tgccctttca
    17161 gcgttaattc aaataacaat gttatatgtt tacctgcgcc aatatcaccc gcatcaacgt
    17221 tgtcgttatt aaaatcctct gccttaagtt ggcgcttttc ataacctatc tgacggtatt
    17281 ccgttaccca cgcgggatta aactcaattt gcgctttgac atcttttgct acggtaatca
    17341 acgtctgccg catttcactg ttcaacactt tctgcgcctc tgagagggta tcaatgtagc
    17401 tatagttgcc gttaccaaca tcggcaattc tcaccatcat tgcctcgtta taattgctat
    17461 cccccacgcc aagcgtcgac agagtaacac cggactcacg ctgttttttg accattgatt
    17521 caatcgattt tggatcgtca atgccaacgt taaagtcacc gtcagtggcc aataaaatgc
    17581 gattgatacc acctttaata aaccccttcg ctgcctgctg ataagccatc tccagcccgg
    17641 caccgccatt ggtactgcct tctgcatcca gcgaatcaat tgcagcatta atttccgcct
    17701 tgtgactacc ggagatagaa ggcaacgcaa tacgagagtc gccagcgtag gtcacgatgg
    17761 caatgttatc ctgctcacga agttctttga ccaataattt caacgaagac tggataagtg
    17821 gcaaacgttc atcagaaatc attgaaccag acgtgtcgat aagaaagacc agattagaag
    17881 cgggtaactc ttcacttttg cgatctttcg ccaggatatc aactttcagc aatgttcgct
    17941 gttcattcca gggtgcaggt gccaattcgt agcgcatagc gaaaggtatt ggcttagagg
    18001 ccggaataga ttgtttgtct ttgatatccc agtcagacgg gaaataattg actatctcct
    18061 ccacccgcac agcgtctggc ggaggtaaca gcccttgatt aaggaaacgc cgcacattcg
    18121 cataactgcc ggtatcgacg tcaaggctaa acgtcgccaa cgggttttgc gctacctgct
    18181 taaccggatt atcatcgaac tgctggtagc gagcggttgc tggatttgcg atttgggagg
    18241 ctgctttttc tctggctgca tgttgatatt ttggagccgc ctgcaatcgc cccagcaaag
    18301 cctgtttgtc tgaatattgt tgcacttctt gctgggctaa ggctttcgcg tctgcagcgg
    18361 ctttggcggc gctttgctca gccacttttg ttgccgcttg ttgcgcggca agcacttgct
    18421 ggtcagtggg tgtagtagat tgttgttgct gacttttctt atcctcaggt tccggcccac
    18481 atcctgacaa aatcaaacta cttataagca acatgattat atttttattt cgcatataaa
    18541 atcct
//
LOCUS       DAJOTB010000093        18360 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJOTB010000093 DAJOTB010000000
VERSION     DAJOTB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.093"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            44..118
                     /gene="tnaC"
                     /locus_tag="OHN28_003153"
     CDS             44..118
                     /gene="tnaC"
                     /locus_tag="OHN28_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCQ2298502.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            339..1754
                     /gene="tnaA"
                     /locus_tag="OHN28_003154"
     CDS             339..1754
                     /gene="tnaA"
                     /locus_tag="OHN28_003154"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCQ2298503.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1846..3093
                     /gene="tnaB"
                     /locus_tag="OHN28_003155"
     CDS             1846..3093
                     /gene="tnaB"
                     /locus_tag="OHN28_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCQ2298504.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3225..4400
                     /gene="mdtL"
                     /locus_tag="OHN28_003156"
     CDS             3225..4400
                     /gene="mdtL"
                     /locus_tag="OHN28_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCQ2298505.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            4375..5334
                     /gene="yidZ"
                     /locus_tag="OHN28_003157"
     CDS             4375..5334
                     /gene="yidZ"
                     /locus_tag="OHN28_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCQ2298506.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5491..6240
                     /locus_tag="OHN28_003158"
     CDS             5491..6240
                     /locus_tag="OHN28_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCQ2298507.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6262..6828
                     /gene="yieF"
                     /locus_tag="OHN28_003159"
                     /gene_synonym="chrR"
     CDS             6262..6828
                     /gene="yieF"
                     /locus_tag="OHN28_003159"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCQ2298508.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6881..8218)
                     /gene="adeP"
                     /locus_tag="OHN28_003160"
     CDS             complement(6881..8218)
                     /gene="adeP"
                     /locus_tag="OHN28_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCQ2298509.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8385..9050
                     /gene="yieH"
                     /locus_tag="OHN28_003161"
     CDS             8385..9050
                     /gene="yieH"
                     /locus_tag="OHN28_003161"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCQ2298510.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9117..9584
                     /locus_tag="OHN28_003162"
     CDS             9117..9584
                     /locus_tag="OHN28_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298511.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9633..10220
                     /locus_tag="OHN28_003163"
     CDS             9633..10220
                     /locus_tag="OHN28_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCQ2298512.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10281..11003)
                     /locus_tag="OHN28_003164"
     CDS             complement(10281..11003)
                     /locus_tag="OHN28_003164"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2298513.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11018..12187)
                     /locus_tag="OHN28_003165"
     CDS             complement(11018..12187)
                     /locus_tag="OHN28_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCQ2298514.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12214..13830)
                     /gene="bglH"
                     /locus_tag="OHN28_003166"
     CDS             complement(12214..13830)
                     /gene="bglH"
                     /locus_tag="OHN28_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCQ2298515.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13916..15310)
                     /gene="bglB"
                     /locus_tag="OHN28_003167"
     CDS             complement(13916..15310)
                     /gene="bglB"
                     /locus_tag="OHN28_003167"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCQ2298516.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15329..17203)
                     /gene="bglF"
                     /locus_tag="OHN28_003168"
     CDS             complement(15329..17203)
                     /gene="bglF"
                     /locus_tag="OHN28_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCQ2298517.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(17337..18173)
                     /gene="bglG"
                     /locus_tag="OHN28_003169"
                     /pseudo
     CDS             complement(17337..18173)
                     /gene="bglG"
                     /locus_tag="OHN28_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
BASE COUNT         4669 a         4550 c         4299 g         4842 t
ORIGIN      
        1 agtattatac tgtagcctcg tgtctttcga ggataagtgc attatgaata tcttacatat
       61 atgtgtgacc tcaaaatggt ttaatattga caacaagatt gtcgatcacc gcccttgatt
      121 tgcccttctg tagccatcac cagagccaaa ccgattagat tcaatgtgat cgattagttt
      181 gctatatctt aattttgcct tttacaaagg ccatctctcg tttatttact tgttttagta
      241 aatgatggtg cttgcatata tatctggcga attaatggat attgcaaatg taatattcac
      301 agggatcact gtaattaaaa taaatgaagg attatgtaat ggaaaacttt aaacatctcc
      361 ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg taccactcgc gcttatcgtg
      421 aagaggcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta
      481 tcgatttact gaccgacagc ggcaccgggg cggtgacgca gagtatgcag gccgcgatga
      541 tgcgtggcga cgaagcctac agcggcagcc gcagctacta cgcgttagcc gagtcagtga
      601 aaaatatctt tggttatcaa tacactattc cgactcacca gggccgtggt gcagagcaaa
      661 tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa
      721 tggtggcgtt ctctaactat ttctttgata ccacgcaggg ccatagccag attaacggct
      781 gtaccgtgcg taacgtctat atcaaagaag ccttcgatac tggcgtgcgt tacgacttta
      841 aaggcaactt tgaccttgag ggattagaac gcggtattga agaagttggc ccgaataacg
      901 tgccgtatat tgttgcaacc atcaccagta actccgcagg tggtcagccg gtttcactgg
      961 caaacttaaa agcgatgtac agcatcgcga agaaatacga tattccggtg gtcatggact
     1021 ccgcacgctt tgccgaaaac gcctatttca tcaagcagcg tgaagcagaa tacaaagact
     1081 ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg
     1141 ccaagaaaga tgcgatggtg ccgatgggcg gcctgctgtg catgaaagac aacagcttct
     1201 ttgatgtgta taccgaatgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg
     1261 gcggcctgga aggcggcgct atggagcgtc tggcggtagg tctgtatgac ggcatgaatc
     1321 tggactggct ggcttatcgt atcgcgcagg tgcagtatct ggtcgatggt ctggaagaga
     1381 ttggcgttgt ctgtcagcag gcgggcggtc acgcggcatt cgttgatgca ggtaaactgt
     1441 tgccgcatat cccggcagat cagttcccgg cacaggcgct ggcctgcgag ctgtataaag
     1501 tcgccggtat ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg
     1561 gtaaacaact gccatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata
     1621 ctcaaacaca tatggacttc attattgaag cctttaaaca tgtgaaagag aacgcggcga
     1681 atattaaagg gttaaccttt acctacgaac cgaaagtatt gcgtcacttc accgcaaaac
     1741 tgaaagaagt ttaattaaaa cactcagagt ggctataagg atgttagcca ctctattacc
     1801 ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac tgatcaatca
     1861 gaaaaaaaac actctgcatt ttggggtgtt atggttatag ccggtacagt aattggcggt
     1921 gggatgtttg ctttacctgt tgaccttgcc ggagcctggt ttttttgggg cgcttttatt
     1981 cttattatcg cctggttttc aatgttacat tcagggctat tattattaga agcaaactta
     2041 aattatcccg tcggttccag ttttaacacc atcaccaaag atttaatcgg taacacctgg
     2101 aacataatca gcggtattac cgttgccttc gttctctata tcctcactta tgcctatatc
     2161 tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta tcacgctaat
     2221 ccacgtattg tcgggatctg cactgccatt ttcgttgcca gcgtattgtg gataagttca
     2281 ttagccgcca gtcgtattac ctcattgttc cttgggctga agattatctc ctttgtaatt
     2341 gtttttggtt ctttcttctt ccaggtcgat tactccattc tgcgcgattc caccagcacc
     2401 actgcgggga cgtcttactt cccgtatatc tttatggctt tgccggtgtg tctggcgtca
     2461 tttggtttcc acggcaatat tcccagcctg attatttgct atggcaaacg caaagataag
     2521 ctcatcaaaa gcgtggtctt cggttcgctg ctggcgctgg tgatttatct cttctggctc
     2581 tattgcacga tggggaatat cccgcgagaa agctttaagg cgataatctc ctcaggcggc
     2641 aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat tatcgaattt
     2701 tgcctgctgg tgttctctaa cttagctgtt gccagctcgt tttttggtgt cacgctggga
     2761 ttgttcgatt atctggcgga tctgtttaag attgataact cccacgccgg gcgtttcaaa
     2821 accgtgctgt taaccttcct gccacccgcg ttgttgtatc tgatcttccc gaacggcttt
     2881 atttacggga tcggcggtgc cggactgtgc gccaccatct gggcggtaat aattcctgct
     2941 gtactggcaa tcaaagcccg caagaagttt cccaatcaga tgttcacggt ctggggcggc
     3001 aatcttattc cggcgattgt cattctcttt ggcataaccg taattttatg ctggttcggt
     3061 aacgtcttta acgtgttacc taaatttggc taaatctttc aggaagccag ccattcgctg
     3121 gcttcttgcc tgtcaggaaa tctcttttgt ccaaatggca actcgcctga ttatccttca
     3181 ccacgtatgc tttgcgtcac cttactatca ggacgcttta gcccatgtcc cgctttttga
     3241 tttgtagttt tgccctggtt ttactttatc ccgcagggat tgatatgtac ctcgttggtt
     3301 taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg
     3361 tttatctggc ggggatggcg gctgcgatgt tatttgccgg taaagtggcc gatcgttcag
     3421 ggagaaagcc tgtcgccata cccggcgcgg cgctatttat tatcgcctcg gtgttctgtt
     3481 cactggctga aaccagcgcg ttatttcttg cagggcgatt tctacaggga ctgggcgcgg
     3541 gctgttgtta cgtagtggcg ttcgccattt tgcgcgacac gctggatgat cgacgtcggg
     3601 ctaaagtgct gtcgttactc aacggcatta cctgcatcat tccggtgtta gcgccggtgc
     3661 tcggacacct gattatgctt aaattccctt ggcagagtct gttctggacg atggcaacga
     3721 tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa agaaacgcgc cctgcggccc
     3781 ccgcagcttc ggacaaacca cgagaaaata gcgagtcact gcttaaccgg tttttcctca
     3841 gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct caccttcgtc aacacgtcgc
     3901 cagtattgct gatggaaatc atgggttttg agcgcggaga atacgccacc attatggcgt
     3961 tgaccgctgg cgtcagcatg accgtttcat tctccacgcc gtttgcgctg ggaattttta
     4021 agccacgtac gttgatgatc acctcacagg tgttattcct ggcggcgggg atcacccttg
     4081 ccgtttcacc ttcccatgcg gtttcgctgt ttggtatcac gctgatttgc gccggtttct
     4141 cggtaggttt tggcgtagcg atgagtcagg cgttggggcc gttttcatta cgcgcgggcg
     4201 tagccagctc gaccttaggt attgcgcaag tttgcggttc gtcactgtgg atttggctgg
     4261 cggcggtggt tggtatcggt gcatggaata tgctgatcgg gattctgatt gcctgtagca
     4321 tagtgagcct gttgctgatt atgttcgtcg cgcctggacg cccggttgcc gctcatgaag
     4381 aaatccatca caacgcttga tctcaatctg ctgctctgtc tgcaactgct gatgcaggag
     4441 cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc cttcgtcggt gagtaagtcg
     4501 ctggcaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc gctggggctg
     4561 tcgcccacac cgctaatggt cagcatggag caaaatctgg cggagtggat gcaaatgagc
     4621 aatcagttgc tggacaaacc actccacgaa acgccgcgcg gtcttaagtt cgaactggcg
     4681 gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt cgaaacggat ctaccaacga
     4741 tacccgcagg cgaccatcaa attacgtaac tgggattacg attccttaga tgccattact
     4801 cgtggtgaag tggatattgg tttttccggt cgcgaaagtc atccccgttc gcgggagctg
     4861 ttaagctcgc taccgttatc cattgattat gaagtgctgt ttagtgatgt gccctgcgtc
     4921 tggttacgca aagatcatcc ggcactgcat gaagcatgga atctggacac ctttttacgt
     4981 tatccgcata tcagcatttg ctgggaacag agcgatacct gggcgctgga caatgtgtta
     5041 caggagctgg ggcgcgaacg cacgattgct atgagcctgc cggaattcga gcagtcactg
     5101 tttatggcag cacagcccga caatctgcta ctggcgaccg cgccgcgcta ctgccagtac
     5161 tacaatcaac tccaccaact gccgttggtt gctcttcctc tcccgtttga tgaaagccag
     5221 caaaaaaagc tggaagtccc ttttaccctt ctgtggcata aacggaacag ccgtaatccg
     5281 aagatcgtct ggttacggga aaccattaaa aatctttacg cgtcgatggc ataaccgaat
     5341 cgtatgaaac gcgacccaat ttcacaatga aatgtaaaaa agttgtaata aatttatctg
     5401 aatcgaactt ttagccgctt tagtctgtcc atcattccag taaatgatta ctcttgtatt
     5461 cataatggac cattaagcat ggagcgaaaa atggcgactc actttgcccg agggatttta
     5521 acggaaggac atctgatttc tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac
     5581 attccccctc atcgtcaaag ccgttttctg gcgtccagag gtttactcgc agaactgatg
     5641 ttcatgctgt atggcattgg cgaattgccg gaaatcgtca ccctgccgaa aggaaaaccg
     5701 gttttcagcg ataaaaattt gccttcgttt tctatttcct atgccgggaa tatggtcggc
     5761 gtggcgttaa caaccgaagg tgaatgtggc ctcgatatgg aactacagcg tgcgacacgc
     5821 gggtttcata gcccacacgc gcccgataac cacacctttt ccagcaatga atcgctatgg
     5881 atcagtaaac aaaacgatcc taacgaagcg cgggcgcagc tcatcacgct gcgccggagc
     5941 gtgctaaaac taaccggtga tgttttgaat gacgatccgc gcgatctgca actactgccc
     6001 attgccggac gcctgaaatg tgctcatgta aatcatgtag aagcgttatg cgacgcggag
     6061 gacgtgctgg tgtggtccgt ggcggtcacg cccacgattg aaaagctcag tgtctgggag
     6121 ttagatggca aacacggctg gaaaagcctg cccgatattc acagccgcgc caacaatcct
     6181 accagccgga tgatgcgttt tgcccagctc tctaccgtga aggctttttc gccaaattga
     6241 tagacaacca caggagtcat catgtctgaa aaattgcagg tggttacgtt actggggagc
     6301 ctgcgcaaag gctcatttaa tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg
     6361 agtatggaag tcaatgcgtt gccttccatt gccgacattc ccctgtatga cgctgacgta
     6421 cagcaggaag acggttttcc agcaacggtt gaagctctgg cggaacagat ccgtcaggct
     6481 gacggtgtgg tgatcgtcac gccggaatat aactactcgg taccgggtgg gctgaaaaat
     6541 gccatcgact ggctttcccg cctgccggat caaccgctgg ctggtaaacc ggtattgatt
     6601 cagaccagct caatgggcgt gattggcggc gcgcgctgtc agtatcacct gcgccagatt
     6661 ctggttttcc tcgatgcaat ggtgatgaac aagccagaat ttatgggcgg cgtgattcag
     6721 aacaaagttg atccgcaaac cggggaagtg attgatcagg gtacgctgga ccacctgacc
     6781 gggcaattga ccgcatttgg tgagtttatt cagcgagtta agatctaaat aaaaacccgc
     6841 cagcaatcat gcatggcggg tttttaacgc gctatcgatt ttagtgagcg tcgataaaga
     6901 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     6961 gtccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcgata ccttcagtaa
     7021 tcgagaagct gaacggcatc atcacagcgg taataaacgc cggaacagat tcagtaagat
     7081 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtaa atcagcgcac
     7141 cagctgcagc gtagcctggc accatccccg ccagcggcga caggaagata accagcagga
     7201 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaacggaa acgccggaag
     7261 aagattcaat atacgccgta acagaagaag taccgataaa cgaaccggtc accgaagaga
     7321 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt acctttctca tcagccagac
     7381 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accagcatga
     7441 aagagaaaat cacccctgcc agcccaaggt taaacgaccc ggctaaatct acatgaccca
     7501 cgacactcat tacgctcggc ggcgcagaaa cgatgccatt atagtggaca tcacccagca
     7561 tccagcccag cagcgttgtc accacgatgg aaaccagcac cgccgcgtga atattgcgcg
     7621 aggccagaat ggcgatgatg aagaagccga ggatccccag cagcacgctg tgagaagtca
     7681 gattaccgat gctcaccagc gtttccgggt tagcgacaat cacacctgcg tttttcagcc
     7741 ccatcatgcc aataaacaga ccgataccac tggtgatgcc cacacgtaga cttaccggga
     7801 tgttggctat catccagtag cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc
     7861 cccagaagat tgcgcccatc ccgacttgcc acggcaagcc catcgcctgt acaacgacaa
     7921 aagcgaagaa cgcattcagg cccattgccg gtgccagtgc aactggcagg ttagcaaaca
     7981 gccccatcat aatactgccg aatgcagcaa tcagacaggt cgtgacgaag acggcgctgg
     8041 tatccatgcc agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca
     8101 ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt
     8161 taaacacgcg ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta
     8221 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg
     8281 gacggctgaa gataactttt tttcattgta tttgcttata cggcaacgat tgcgtcatgc
     8341 gaattggcat gacgaaaacg ttaaactaac gataaaggaa agacatgtcc cagatagaag
     8401 cggtattttt cgactgcgac ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcct
     8461 atgtcacgat gttcagggaa tttggcattc acgttgacct ggaagagatt tttacccgtt
     8521 ttaaaggcgt aaaactctac gaaatcatca acatcatcag taaagaacag ggcgtgacga
     8581 tgatccaggc cgatgccgag cacgtttacc gtgcggaagt ggcgcgcctg tttgatagcg
     8641 agcttgaggt gattgcagga gcgaacgaat tgctggctag tgtgaaagtg ccgatgtgcg
     8701 tcgtctctaa cggcccggtc agcaagatgc agcattcgct tggtaagctc aacatgctgc
     8761 actacttccc ggaaaaactg ttcagcggct atgatatcca gcgttggaag cccgatccgg
     8821 cattaatgtt ccatgcggca aaagcgatga atgtgaacgt tgaaaactgc attctggtcg
     8881 atgactccag tgccggtgcg caatcaggga tagatgccgg aatggaagtg ttttacttct
     8941 gcgctgaccc gcacaataaa ccgatcgttc acccgaaagt caccaccttt acccatcttt
     9001 cgcaattacc ggaactgtgg aaggcgcgtg gttgggatat tacggcatag ttattcacac
     9061 tcccttcact tactccactt aaattggcac gcaaaggtaa gtaaagggag tttgatatgt
     9121 ctgtttcacg tcgggtaatt catcacggac tgtattttgc ggttttaggg ccgttaattg
     9181 gtgttctgtt tcttgtcctc tacatattct tcgcaaaaga accgctggtt ctttgggtga
     9241 taatacatcc aatttttctc ttattgtcga taactacggg agctattcct gcgttgttaa
     9301 ccggtgtaat ggttgcctgt ctgccggaaa agatcgggtc acagaaacgt tatcgttgtc
     9361 tggctggtgg cataggtgga gttgttatca ccgagatcta ttgcgctgtt atcatacata
     9421 ttaaggtcat ggcttcctca gtgttgtttg aaaacattct ttctggtgac agtctcgttg
     9481 tccgcatcat tcctgcattg ctggcaggtg tggtgatgag cagaatcatt acacgtctac
     9541 ccggactgga tatttcatgt cctgaaacag actctttaag ttaagcggga tgttttatct
     9601 tttggctact caaaagcaga caggatgttt ctatgactca aaatatcagg ccgttacccc
     9661 tattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat aaaaccgtag
     9721 agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt tattgcgttg
     9781 atgaagttga acatctctgt ccgtggtgta ttgcgaacgg ttctgctgct gaaaagtttg
     9841 caggtagttt tcaggatgat gccagcatcg aaggtgttga atttgagtat gatgaagagg
     9901 acgattttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag ttggttgaac
     9961 gcacgccagg ttatcatgga tggcaacagg aattctggct cgcgcattgt ggcgatttct
    10021 gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat gaatttgcca
    10081 accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct aaatgcctgc
    10141 aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc aagctgagac
    10201 tgtggggcga tttttcatag ttatttatat gatgagaaca ggccggcgcg taattcacac
    10261 atccggcctt atttttaagc ttaaccgagc gctaattctg cggcagcggc tttatcagcg
    10321 attaccatca gcgaaggatg cagctgcaac acggaagcag gaacatcttc ggtaaccgga
    10381 ccttgcagaa catttttcag tgcctgcgct ttacccgcgc cactgacaat tatcagcaag
    10441 tttttcgccg ccatgatgct tttcggcccc atcgtcacgt agctgtcagg cactaatgaa
    10501 aagtcgccac ccaactcgcc atgcgccaca atatcaacca tctctccctg gatcgggaac
    10561 tccacggttt gctcatggaa atgggtcgta tttggcaaat tgccacaaaa atgaccatct
    10621 acacctaatc ccaacaccac caaatccagg ccaccttcac gcgccagttt ctgatcatgc
    10681 tcgcggtagt tatcaatagt gagcttctgg atattctctt ctttgatccc tgcaggggtg
    10741 aaaaacagat tacgcagatt ggtaatcgtt acgccttccc cttctttgcc acgaaatgga
    10801 atttcatcga aattgtagaa atagcagtta tcgtaccagg gcttaccttt aaccagagtg
    10861 atgagatatt cgtacatgcc tttgggcgtg ctaccggcgg taattgccag gttaacgcga
    10921 cgcatctttg acatataacc aagcagatga tgtgcggcga cacggctcat ttcctggtaa
    10981 tcttcggtaa tgatcaattt catcttaatg ccttatttta tttaaataat ttctgtacaa
    11041 aggctgcata ggctggtcgc cagacatcca tttcatggtt cagaccggga tattcctggt
    11101 aatcaaactt tatttttttc tgctcaagct cagttttcag cccggcgata tccttgccgg
    11161 ttacgacatc tttatcgccc acgaccacag taaaattacg tagttgctgg ttaatagctg
    11221 ccggatcgtt cagccgggca gcgacccctt catctggtac ggtcgtcgtg gtaacaccac
    11281 tgaatgtggc cagccagcca aagctttcca gatgattaat tcccgatacc agtgcctgat
    11341 acccaccttg tgaaagccct gccagtgcgc gaccatcggc atctttacgg acattaaaac
    11401 gcttgctaat cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattta
    11461 acggataaaa gactttacgt ctctcctgag gaacgaaatc ttcgggaata atgcccttcg
    11521 catcggtttc ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca
    11581 ggttatccat gatttgcggg atacgcccct gatcaatagc ggaacgtccg gtatcaccaa
    11641 agccgtgata gaaatagagc actggcaaag gctcgcctat gccggtgtat cctggcgggg
    11701 tccagacata catctgacgt tcggattgca atgcgttgga gtggtaagtt atggcgatca
    11761 aatcaccatg cacaacagaa cgtgtatcca gataactgcc cggtaccaga atcatactgg
    11821 agttaacctg acgctgaggc ttagtcattg ccgtgcctgt atcaatgctg cgtacaccat
    11881 caacattgaa aaaatactcg tacagattgc ctttcaggac aggcgtgcgc cacgaccaga
    11941 ctcccgcttc atctttggtc atcgggtgaa tattgtccgg aaccggaaca cccaccacta
    12001 cggagacatt ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggtattga
    12061 cctgagtcac atactgtttc accggaatag tgggtgccgg gctggctggc atatccgctg
    12121 ctatggccca ttgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta
    12181 tattcatcgt aatattcctt ttattaagtt ccattaccac cagatttcag cctgggcacc
    12241 gacagcaaac tggtcgtctt tattgtcttc aaaggtgaag ccatccagtt cgttttccag
    12301 agctttgata tatgtagcgt agaaacgaat ttccgggcgc gaggtcaaca tactggtatt
    12361 gactttaaag gtatggaaga gcgtggtttt ataaccagac tcattatatt tattgctatt
    12421 cgcatctttg ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc
    12481 ccaaatatag gccgggcgaa caaccgcacg aatagattcg aaatcagaat gtgcgcctgt
    12541 ttcgtagcta taaatatcgt taccgaagga gtaaacaatc gcgttagcca caatgaaatg
    12601 atcgccgata taagcttcgc cctgcgaagt cagacgaact gcagttccgc ctgtgtgatc
    12661 accataataa cgaccattaa aggtagtaaa tggacttgca ccagcataac gaccaaagtt
    12721 actggcgata gagttattcg caaccaggaa ggagaattcg ttgaagccac ccttatcaaa
    12781 tttctgcgtt aaagatgtac caaacatcca ggtatctttc cagtcataat acccgttatt
    12841 atcctgatta tctttttccg atgcgctttc atttgctgag acataacgac cgctcaccat
    12901 taaggtggct ttatcccata acgggatatc tttatagcgt aaatcaatgg tattggtatt
    12961 aatctgctgt ttgttttgca ggctgcgatc gtaatcatcg acatcttcac gaaccagcgc
    13021 gatatcaatt ttacccggac cgactttcca gttttccaga cctacacctg cggcagcatc
    13081 ggtacgctgc gttttccagt caagcatctg gatttcaatt ttcggcgcac cgtgtttacc
    13141 cacccagaaa tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc
    13201 agagaactgc ataaagttct cgccaccggc gttatcgcca aaccagccag tagagtactg
    13261 ctgacccacg ttaccatcca tcatcacgat ggcatcaacc cgtttgccgt tttcgttgta
    13321 gacacgttgt tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag
    13381 agaaccaatt gcccatgatt taggtgaacc atgggaggct gtgccccaac cagaacggta
    13441 gtagccgttg taactaaagc cgatttcatc tttcacaaat ttactgaaat ctttcagagt
    13501 catggaagaa taaatcgagc ctgtctcact ggcatttttc tcttcatttt taaccagtac
    13561 agcatcaggc tttgcggcac gactggtcgg gaatggattg gcggcatacc cttgatcatt
    13621 cgcacttacg ctacgattca ccgtcgctgg cgtatacttt ttcttctctt catctttata
    13681 ttttttcagt tcgctttgcg tttctcttaa cgccttttct aatagctcaa ggcgttgttc
    13741 cacggttaat gattcagcca atgattgtgc ggaaaaggct aacggtgcca ttaataaaat
    13801 ggcagaggta ataatatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt
    13861 aacgcatcgc atccagactg ttctgaatgc gacgatattt aaggtgcttt attggttatt
    13921 tttttaatga tagcccccga gttttgatca cttctgcata ccatccgaag cttttcttgc
    13981 gcgttcttgt gaggcttcct tcaccattat catcacgatc cacataaata aagccgtagc
    14041 gcttagacat ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc
    14101 ccataatatc cacaccatcg gcaatcgctt catttacctg taccaggtga tcatttaaat
    14161 aggcaattcg ataatcgtcc tgtatcgaac catccacttc aacgctgtct tttgcgccta
    14221 atccgttctc gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa
    14281 tccgtaatcc aaccggatca atttgccacc cccactctga acttttcaga tgcggatttg
    14341 ggatcatatt cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac
    14401 aaccggtcat gtaataacta aaagagatga aatctacggt atgttttaaa tcttctgcgt
    14461 cactttcagt catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg
    14521 gatactgacc acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg
    14581 cctgcaacat atcctgtggc tggcaggtga gggggtaaac cagcccaccg agaagcatat
    14641 tgccaatttt cgcttcgggg atcaggctat gacaggcttt aactgcccgc gcactggcaa
    14701 ccagttgatg atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca
    14761 cgcccgtgaa tggcgcgtgt aatgacatgt tgatttcatt aaatgtcagc cataacacaa
    14821 ctttatgttg gtagcgggta aagaccgtgc gtgcgtaatg ctcgaagtga tcgatgaccg
    14881 cccgattagc ccaaccgccg tagtttttca ccagcccata tggcatttcg taatgggata
    14941 acgttaccag cggcttgatc cccgcctgcg ccatttcatc aaacagccgc tcgtaaaacg
    15001 ctaaccccgc ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa
    15061 tggaaatacg cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac
    15121 ggtgataaaa atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca
    15181 tttttcccat tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcct
    15241 gccatgcgcc ttccacctga ttggcagctg ttgcaccacc ccaaagaaat gtttctggaa
    15301 atgctttcat aattaactcc ttttatcgtt agcgaatgat ggataacagc ggctcaccag
    15361 cgtttatctg caccgtgccg tggggtaata cgtccgtaaa atcatcgcta ttactgatta
    15421 ataccggcgt cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca
    15481 gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt
    15541 ccagttttac ggtgtcgata ccgacatgaa tcaggatctc cacgccatca tctgactcaa
    15601 tgccaatggc gtgtaatgtg gcgaacaacg aagcaattcg acccgccacc ggagaacgca
    15661 cttcaccaac agagggcaga atggcaatac ctttacccaa caggccactg gcaaacgtgg
    15721 tatcagcgac gtgaatgagc ggcacaatat ctcccgtcat cggtgaacag ataccgccct
    15781 gctcaggtgt aataatctct ggtgttttct cttgctgggc aacttgcgct ggctgacgtt
    15841 tagcggtgat gaaatgaagc atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac
    15901 caccaataac gctggcccat acggtgaaat caatccccgt tgacgggatg gtttgcatga
    15961 aggtgaaaat acctggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    16021 tggcccccaa tgccccactt atgcaggcga taacgaaggg gtacttacgc ggcaggttga
    16081 cgccatatac tgccggttcg gtgataccaa acagccccgt caacgccgcc gatcccgcta
    16141 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacttgcg
    16201 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    16261 tgataattaa aggtatcaaa ccccaatgca gaccaaacat cacaaagatt tgccagaagc
    16321 cgcccattac cgcgcctgca aatgcaggaa ccgcctgata aagccagaga taaccggcag
    16381 caatcagttc acttatccag gttgatagcg gccccaccag cagaaatgtg atgggcgtga
    16441 tgaccatcag acatagcaag ggtgtgaaga aatttttgat tgccgaaggt aaccacgcat
    16501 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    16561 acgagtaatt caacaatgtg accggaatac ccaggaaatc caggcccagc gcatccactt
    16621 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctccg ccaatcacca
    16681 tggcagtaaa tggattgccg ctgaagcgtt tccctgcggt gtagcccagg attatcggga
    16741 agaaccaaaa caaggcatca ctggcgctaa ataaaattaa ataagtacca ctttgttcgg
    16801 tcgtccactg aaaagtgagt gccagagcca acataccttt caggatcccg gttgccgcca
    16861 tcaaaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttaacagat
    16921 tacctttatc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    16981 taaccgccag gaagacatcg gccacatggt tacctatgac aacctgaaac tggccaccgc
    17041 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    17101 catcctttaa cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg
    17161 cgcccccgac tcctgcgact attgttctgg ctaactccgt cataacttgc cctctaccgc
    17221 tttgcggcaa aactcaaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    17281 tttttcaggt tttgcccgct tagtgcggta acaatccttt attcagtaat aatatttcag
    17341 tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc aggtgagatt
    17401 ttacgttgat actgcaaacc aataaaaatg gcgatccgct ccgcacattg ccatgcttgc
    17461 gggtaatttt gctttactgc ttattgtaat gattcatcac tatcgttaat cgatgcatgt
    17521 tcaataatac gccaggataa aaatttcaga tgcgtaacca gtcgctgata actcaagctt
    17581 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc tcgcattaat
    17641 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    17701 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    17761 aacgcttctt cccctagctg gaactctttc gggtaaagtc gctggatatc ccacagcagc
    17821 gggttgggca gtagcacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    17881 agcgagatat aaatactgtc ctgcaacttt cccagacgct cctgcgccag agagataata
    17941 cgatcacagg ttgccatcac ctcaagaggc atatgactta agagttcgct taatcgcccg
    18001 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    18061 cctgggcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    18121 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    18181 acctttttta taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt
    18241 gcctgcgaat gcagtaacaa tccagtcatt aattaatggt ttttataacg aacatccagg
    18301 ttcgaaaatt aatttagttg cgtgcagaaa aaatagcaat gctctattta taaaagtatg
//
LOCUS       DAJOTB010000094        18337 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJOTB010000094 DAJOTB010000000
VERSION     DAJOTB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.094"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            25..815
                     /locus_tag="OHN28_003170"
                     /pseudo
     CDS             25..815
                     /locus_tag="OHN28_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..1402
                     /locus_tag="OHN28_003171"
     CDS             1196..1402
                     /locus_tag="OHN28_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2298518.1"
                     /translation="MNTPVSLMDDQLVDMAYITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1489..2097
                     /locus_tag="OHN28_003172"
     CDS             1489..2097
                     /locus_tag="OHN28_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298519.1"
                     /translation="MKMKPQYQTRYESLHESYLKWLTGFTRHAVSWGMCHPNIYYFHN
                     LMAGWISFNGEKPEIAIVPQSLHRIINGNDKKTIPPLDDDLVVSLCTSEHLLVHHPML
                     EGVLLSECERLGQHSLANRLISLFRQFSGTELRLKLIWLCWYDLMIGNSLKDWTENMK
                     RKSEKELAEWVIDRQTENEPLTYLMDQYVLLAYRTTVDTAHY"
     gene            3546..4553
                     /locus_tag="OHN28_003173"
     CDS             3546..4553
                     /locus_tag="OHN28_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298520.1"
                     /translation="MAKEYPYAPTGPVKTRDLRVCKFYAKVLKTEFNNFPDIPCQDIE
                     ICRYELNNAIHKYATNEFAIKSLMFALESKREENILPDESFNWLKKNERATFWLWATI
                     HNMDDIELKYMCDYSNENLRDGLSATWYQRLNLSLSPTNHQDRINLIIAFMDELIFPA
                     PPVMHRKKTLMENLKQKWKTVFARPLPLKWLPVNDEDAVLWAWESLKKHQSGTFGSFG
                     NTGPLLTQWFSPISHSERVLALQAALDIWDAPDSRRLFLLNLNKAWNQQKLRKSRTDK
                     KAINTYLKNDTKTRLNLMAEHNRLRIGDMLEKLINEYYQEHYCRQHNHTETKNPADDR
                     F"
     gene            4654..5526
                     /locus_tag="OHN28_003174"
                     /pseudo
     CDS             4654..5526
                     /locus_tag="OHN28_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            5899..8745
                     /gene="ag43"
                     /locus_tag="OHN28_003175"
     CDS             5899..8745
                     /gene="ag43"
                     /locus_tag="OHN28_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCQ2298521.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVSGGTLENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAVATDTVVNTGAEGGPDAENGDTGQTIYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAWKTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVSLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSISGSSAEGYNGQATLNVTF"
     gene            8866..11382
                     /locus_tag="OHN28_003176"
     CDS             8866..11382
                     /locus_tag="OHN28_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298522.1"
                     /translation="MLQIVGALILLIVGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYSFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNCFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDISEGSVVSLAAGGISFALTAS
                     LFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIPQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            11458..11913
                     /locus_tag="OHN28_003177"
                     /pseudo
     CDS             11458..11913
                     /locus_tag="OHN28_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11992..12225
                     /locus_tag="OHN28_003178"
     CDS             11992..12225
                     /locus_tag="OHN28_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2298523.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRWVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDALLRQ"
     gene            12325..13143
                     /locus_tag="OHN28_003179"
     CDS             12325..13143
                     /locus_tag="OHN28_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2298524.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQIRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVSVFD
                     RIEEKRDAMQSLLLPPPARQALAQAALTYRYGDEHQPITTADILTPRRREDYGKDLWS
                     AYQTIQEDMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            13143..13388
                     /locus_tag="OHN28_003180"
     CDS             13143..13388
                     /locus_tag="OHN28_003180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298525.1"
                     /translation="MKHIPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            13482..13955
                     /locus_tag="OHN28_003181"
     CDS             13482..13955
                     /locus_tag="OHN28_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2298526.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGTFMAPEPDDDNDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            13970..14446
                     /locus_tag="OHN28_003182"
     CDS             13970..14446
                     /locus_tag="OHN28_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2298527.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFQVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALHHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            14509..14730
                     /locus_tag="OHN28_003183"
     CDS             14509..14730
                     /locus_tag="OHN28_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2298528.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            14749..15393
                     /locus_tag="OHN28_003184"
     CDS             14749..15393
                     /locus_tag="OHN28_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HCQ2298529.1"
                     /translation="MSRIITTTVYTLNELSCPAREKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPAAPRKIR
                     EYAPQDHELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     ADAEAVVSEALRDLAFWLYSQLENEYDSLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            15443..15811
                     /locus_tag="OHN28_003185"
     CDS             15443..15811
                     /locus_tag="OHN28_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2298530.1"
                     /translation="MSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCKAD
                     TLGSCGYVYLAVYPTSETKK"
     gene            15900..16276
                     /locus_tag="OHN28_003186"
                     /pseudo
     CDS             15900..16276
                     /locus_tag="OHN28_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            16273..16497
                     /locus_tag="OHN28_003187"
                     /pseudo
     CDS             16273..16497
                     /locus_tag="OHN28_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16440..16622
                     /locus_tag="OHN28_003188"
     CDS             16440..16622
                     /locus_tag="OHN28_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCQ2298531.1"
                     /translation="MHSTTVYDIAWLQDNIDCESEIIFDNNEDKTDSAALLPCIEQAR
                     EDVRTLRHLQLIHLSA"
     gene            16707..17542
                     /locus_tag="OHN28_003189"
                     /pseudo
     CDS             16707..17542
                     /locus_tag="OHN28_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            17884..18054
                     /locus_tag="OHN28_003190"
                     /pseudo
     CDS             17884..18054
                     /locus_tag="OHN28_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         4777 a         4696 c         4564 g         4300 t
ORIGIN      
        1 atatgattaa cagaaaattt catcatgaca acacgtataa ctttcaatat gaccagtgat
       61 gagactctgc gaattgtgga tgagtactgt catacccaca aactttcgcg ctctaaggta
      121 atcaacgcat tactcagcgc cacagcacct gtccttaacg atattaactg ttattatcaa
      181 cttgcaggcg aactgcagtt acgcttatta agtggagtgt atcaacggaa tcttcctcac
      241 aaacgcaatg tcgtcagtgc agagaagtat tgtctagaaa tctgggaaaa caaacttttc
      301 accagaagaa tcctggagtt tgacagttca aatggtgttc tgtacgcgct gaaacataaa
      361 agacactatc gccgggataa gatgattgga cgggttgaaa gccggcgtat caaagacatt
      421 tgcgaatacc agatgcaact gtccggggag aaggcaaaat atgcctgttt tatttatatt
      481 gaaagaacaa tatacaatca cgataatcca tccggtggga cacctgtaaa agcggctgtt
      541 ggtaatgcag tcatcctgct ggcaaaagac gtgatatatg acgagtactt ttttgatctg
      601 cggcagtcat ttttcgtgag cgttaaagat ctgatggcct ctggcacaaa aggtattccg
      661 gaaacacaga aatatccgaa cgtttattgc tggttcccgc ttttctccat aagttacggc
      721 gtggtcatta ccccggtcta taaaattgat cccctgaaac ccgtaacggt aaaaaaacgg
      781 ataagatcac tgttgtttgt aattacagag aataatttcc gtcttctccg ttcaccgcaa
      841 cagtgtctac ctcgctttat cttccgttca gtaaatacaa accaccgctc tggcaggtac
      901 agggcgtggt ttatctccga tactccccag cgcagtaata ataatcactg agactactga
      961 ttccgtcacg gcaaaatgcc atcccatatt ccggcaacaa caacctcttc cgtttatacc
     1021 ggcttcgctg acaaaacaga caacctgtat tgcctgtaca caacagttta tctcctggca
     1081 ggaatattct cctactcatt ttttacgttt ctccccggtt gttatgccgg tgaaggtggt
     1141 gcgtttttca tcaccaacgc cggttgagat taacaacacc cggaggaaca ttttcatgaa
     1201 taccccagtt tcgctgatgg atgaccagct ggtcgacatg gcgtacatca ctcaactgac
     1261 cggcttaacc gataagtggt tttacaagct catcaaagat ggggcctttc cggcccccat
     1321 caaacttggc cgcagctcgc gatggctgaa aagtgaagtg gaagcttggt tgcaggcgcg
     1381 tattgcacaa tcccgtccgt aattattaac cgttatccgt tcacctgcag cagacgcaat
     1441 ttccggcctg ccggggcatt ctgctgcctg ttataccccg taaggaatat gaaaatgaaa
     1501 ccacagtatc agacccgtta cgaatcgctg cacgaaagct acctgaaatg gctgaccggc
     1561 ttcacccgac acgccgtatc ctgggggatg tgccatccga acatctatta cttccataac
     1621 ctgatggccg gatggatatc attcaatggt gaaaaacctg agatcgccat tgttccccag
     1681 agcctgcacc ggattattaa cggtaatgat aaaaaaacca taccgcccct ggacgatgat
     1741 ctggtggtaa gtttatgtac cagtgaacat ctgcttgtgc atcatccgat gctggaaggt
     1801 gttctgttgt ctgaatgtga gcgcctggga cagcattctc tggcaaacag actgatcagc
     1861 ctcttccgtc agttcagtgg taccgaactg cgcctcaagc tgatctggct gtgctggtat
     1921 gacttaatga tcggcaacag cctgaaagac tggacagaga atatgaaacg caaatcagaa
     1981 aaagaattgg cggaatgggt catcgaccga cagactgaaa atgaacccct gacatatctg
     2041 atggatcagt atgtgcttct ggcataccga acaacggttg atactgctca ctactgatgg
     2101 ccctttagtg ataccggaag gtacaattcc ggggcagcag aggagactcc cgacagcccc
     2161 ggcagcaaag cacagtggaa ctccccccgt taacgtttct ccggtcctaa gacatcaatc
     2221 atctgttctc caatgactgg tctaaaaacc agtagtaaga ctatcattta tttaagtgat
     2281 actggttgtc tggagattca gggggccagc tcacattgaa taataatatg tgtatcacac
     2341 tgaagcatta taactttttt agcgtatctg catccgctga caacatgatt tacatatacc
     2401 accaatgaaa atacgcagat gttaacgaga gcctgttgac gctcatctgt ccatactgaa
     2461 aaaatgccaa atcccctcat taattctctg tgttcagatt tctgaattgt cactcagcat
     2521 aatcacatgc gcccgcttac atctgatatg tcccctctca tccagattta cgtgctgaat
     2581 accagtccag caaaggataa taaaaaagcc aatgcactat ctgtcgtgcc ctgaatgtat
     2641 accgggacat catcaaaagt tacttaacca gaaaaaaacc tgtaagtcgt tttttcacgc
     2701 ctgcacagaa ataatctttc ctttacagca tcgcttcctt tcttcttaca gaatttgctc
     2761 cgttgaaaaa taacaacata aaatttgtct tttattcaga gtaaccagaa catttatcct
     2821 gagcagtttc ccccggattt cctgcaattc cgtcaatacg tttgtcagat aacattcaaa
     2881 tgcggaccgt atactaaagt gtataaaaac atgatttctg cacagtaata ttcgccccaa
     2941 accttaataa atcaggacag acaattacgg cgatatcatc actgttttca gtcagtgtat
     3001 tgtatcctcc tgaaagcaac tttacatttt attgcgttgt ggactacttc cgttgtaata
     3061 ccctcatcac gcacccaaaa tacgtcaacc tgataaagta cattaaaaat acatacctcc
     3121 accggtaaca ctgcatattc ctgttaatag cctccgatac ggatcccccc ctgttttgca
     3181 ggcaacaaag aagcgacact gtcacaacct ctcaccatca ccatgcaacc ggataaaata
     3241 aaaaatgcgc ttccggaata ttaccgaaga gatggtcggt accggccacc tcatatcact
     3301 cccgcccctg ataaacaaca gagaaacctg ctaaaaagag agctatccgc agagaaaaca
     3361 tcagaaaaac gcgccaatga acaccatgat acaaccagtg aaaaacctta taaaagccct
     3421 tgtattaaca caatatcact acaggatgac taccagagtg actttcaggt gacttgacga
     3481 acacaacaaa accattgtat cactactaaa agtatcacta cgtagtttgt atgagacatc
     3541 agataatggc taaagaatat ccatacgccc ccacaggacc tgtaaaaacc cgggatttgc
     3601 gggtgtgcaa attttatgca aaagttttaa aaaccgaatt taataacttt cctgatatcc
     3661 cctgtcagga tatcgaaatt tgcagatatg aattaaacaa cgcaattcac aaatatgcga
     3721 ccaatgaatt tgccataaaa agtttaatgt tcgccctaga gagtaagcgg gaagaaaata
     3781 ttcttccgga tgaatcattc aactggttga aaaagaatga gagagccaca ttctggttat
     3841 gggcaacaat ccacaacatg gatgacattg agctaaaata catgtgtgac tacagcaatg
     3901 aaaacctgcg tgacggtctt tccgccacct ggtatcagcg tttaaattta agtctgtcac
     3961 caacaaatca tcaggacaga ataaacctta tcatcgcatt tatggatgag cttattttcc
     4021 ctgcacctcc agtgatgcac agaaaaaaaa cactgatgga gaatctgaaa caaaaatgga
     4081 aaacggtctt cgccaggcca ttgccattaa aatggttgcc ggttaacgat gaggacgctg
     4141 tattgtgggc ctgggaatct ctgaaaaaac accagtccgg aacattcggt tctttcggga
     4201 atacaggtcc tctactgact caatggttct ctcctatcag ccattcagaa cgtgttctgg
     4261 ctttacaggc ggctctcgat atctgggatg cacctgacag cagacgctta tttttgctta
     4321 atctgaataa agcctggaat cagcaaaaat tacgtaaatc ccgcacggat aaaaaagcaa
     4381 tcaacaccta tctgaaaaac gatactaaaa cccggctcaa tttaatggca gaacacaaca
     4441 gattgcgtat tggagacatg ctggagaaac tgattaatga gtactaccag gagcattatt
     4501 gcaggcagca caaccacaca gaaacaaaaa acccggctga cgacagattc tgaaatacat
     4561 tcaaaataat ttcagacaga tattatcgtt gtgaatcccc cgtcaccttt acggtgtgcg
     4621 ggagttttgt cttttttcac cgggaataca tatatgaatc cgtctgatgc cattgaggca
     4681 attgaaaaac cgctctcctc cctgccttat tcactttccc gtcacatcct ggaacatctg
     4741 cgcaaactca ccagtcacga acccgtgatt ggcattatgg gtaaaagcgg ggccggtaaa
     4801 tcctcactct gtaatgcact gtttcagggg gaggtcaccc cggtcagtga tgttcacgcc
     4861 ggcacccggg aagtgcggcg tttccgtctg agtggccacg gtcacagcat gattatcact
     4921 gacctgcccg gggtgggcga gagccgtgac agggatgcag agtatgaagc cctgtaccgt
     4981 gacattctgc ctgaactgga cctggtactg tggctgattt aagccgatga ccgtgccctg
     5041 tctgtggatg agtatttctg gcgacacatc ctgcaccggg gacatcagcg ggtgctgttt
     5101 gtggtgacgc aggccgacaa aacggagccc tgccatgaat gggatatggc cggtattcag
     5161 ccttctcctg cacaggcaca gaacattcgc gaaaaaacgg aggcggtatt ccgtctgttc
     5221 cggcccgtac atccggttgt tgccgtatcg gcccgcaccg gctgggaact ggatacgctg
     5281 gtcagtgcgc tcatgacagc gcttcccgac catgccgcca gtcccctgat gacccgcctg
     5341 caggacgagc tgcgcacgga atctgtccgc gctcaggccc gtgaacagtt taccggtgcg
     5401 gtggaccgga tatttgacac ggcggagagc gtctgtattg cctctgttgc atgcacggtt
     5461 ctgcgtgccg tccgtgacac ggtggtctct gttgcccgcg ctgtatggaa ctggattttc
     5521 ttctgaacac tctgccaagg gaaatgcctg attacattgc gttaaccaac tgaaaacatg
     5581 acacaaacac ctcatccccc tcctgtttct cttgcgtgac cgccctgcta tcaatagaat
     5641 aaaacgatcg ataaaacagg tatcaatcgt tactatcgat cgtttatagt ggttaatggc
     5701 ctgcgaaaag gcagctgaat ctcttcatca tgcagaacgg aattgcacac aacggactga
     5761 tacttctctg gcggatgaga gaggggaagc atttatgtcc gggaaaaacc actttatctg
     5821 accatgctgt tttgtacctg ccggtatcca catttgtggg taccggcttt tttattcacc
     5881 ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat
     5941 cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg aaaacgcgcc
     6001 ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact ggctgctgac
     6061 acggttgtac aggcgggaga aaccgtgagc ggcggaacac tggaaaatca tgacaaccag
     6121 attgtcttcg gtacggccaa cggaatgacc atcagtaccg ggctggagta tgggccggat
     6181 aacgaggcca ataccggcgg acaatggata caaaatggcg gtatcgccaa caacactact
     6241 gtcaccggtg gtggtcttca gagagtgaat gccggaggaa gcgtttcaga cacggttatc
     6301 agtgccggag gcggacagag ccttcagggg caggcagtga ataccactct gaacggcggt
     6361 gagcaatggg tacatgaagg cgggattgca acgggtaccg tcattaatga gaagggctgg
     6421 caggccgtca aatccggcgc agtggcaacc gacacggttg tgaataccgg cgcggaaggg
     6481 ggaccggatg cagaaaatgg tgataccggg cagaccatct acggagatgc cgtacgcacc
     6541 accatcaata aaaatggtcg tcagattgtg gctgctgaag gaacggcaaa taccactgtg
     6601 gtttatgccg gcggcgacca gactgtacat ggtcacgcac tggataccac gctgaatggg
     6661 gggtaccagt atgtgcacaa cggcggtacg gcgtctggca ctgttgtaaa cagtgacggc
     6721 tggcagatta tcaaggaagg tggtctggcg gatttcacca ccgttaacca gaaaggcaaa
     6781 ctgcaggtga acgccggtgg tacagccacg aatgtcaccc tgaagcaggg cggcgcactg
     6841 gtcaccagta ctgcagcgac ggttcttggc agcaaccgtc tggggaattt cacggtggaa
     6901 aacggtaagg ctgacggtgt tgttctggag tccggtggtc gcctggatgt actggaggga
     6961 cattcagcct ggaaaacact ggtggatgac ggcggtaccc tggcagtgtc tgccggtggt
     7021 aaggcaacag gtgtcaccat gacatccggt ggtgccctga ttgcagacag tggtgccact
     7081 gttgagggga ccaatgccag cggtaagttc agtattgatg gcatatccgg tcaggccagc
     7141 ggcctgctgc tggaaaatgg cggcagcttt acggttaatg ccggaggact ggccagcaac
     7201 accactgtcg gacatcgtgg aacactgatg ctggctgccg ggggaagtct gagtggcaga
     7261 acacagctca gtaaaggcgc cagcatggta ctgaatggtg atgtggtcag taccggcgat
     7321 attgttaacg caggggagat ttactttgat aatcagacaa caccggacgc cgtactgagc
     7381 cgtgctgttg caaaaggcaa cgccccggta acgttccata aactgacaac cagtaacctc
     7441 accggtcagg gtggcaccat caatatgcgt gtcagccttg atggcagcaa tacctctgac
     7501 cagctggtga ttaatggtgg tcaggcaacc ggcaaaacct ggcttgcgtt tacaaatgtc
     7561 ggaaacagta acctcggggt ggcaacctcc ggacagggta tccgggttgt ggatgcacag
     7621 aatggtgcca ccacagaaga aggtgcgttt gccctgagtc gcccgcttca ggccggcgcc
     7681 tttaactaca ccctgaaccg tgacagcgat gaagactggt acctgcgcag tgaaaatgct
     7741 tatcgtgcag aagtccccct gtatgcctcc atgctgacac aggcaatgga ctatgaccgg
     7801 attctggcag gctcccgcag ccatcagacc ggtgtaaatg gtgaaaacaa cagcgtccgt
     7861 ctcagcattc agggcggtca tctcgggcac gataacaacg gtggtattgc ccgtggggcc
     7921 acgccggaaa gcagcggcag ctatggcttc gtccgtctgg agggtgacct gctcagaaca
     7981 gaggttgccg gtatgtctgt gaccgcgggg gtatatggtg ctgctggcca ttcttccgtt
     8041 gatgttaagg atgatgacgg ttcccgcgcc ggcacggtcc gggatgatgc cggcagcctg
     8101 ggcggatacc tgaatctggt acacacgtcc tccggcctgt gggctgacat tgtggcacag
     8161 ggaacccgcc acagcatgaa agcgtcatcg gacaataacg acttccgcgc tcggggctgg
     8221 ggctggctgg gctcactgga aaccggtctg cccttcagta tcactgacaa cctgatgctg
     8281 gagccacaac tgcagtatac ctggcaggga ctttccctgg atgacggcca ggataacgcc
     8341 ggttatgtga agttcgggca tggcagtgca caacatgtgc gtgccggctt ccgtctgggc
     8401 agccacaacg atatgaactt tggtaaaggc acctcatccc gtgacaccct gcgcgacagt
     8461 gcaaaacaca gtgtgagtga actgccggtg aactggtggg tacagccttc tgttatccgc
     8521 accttcagct cccgggggga catgagcatg ggtacagccg ccgccggcag taacatgacg
     8581 ttctcgccgt cccggaatgg cacgtcactg gacctgcagg ctggactgga agcccgtgtc
     8641 cgggaaaata tcaccctggg cgttcaggcc ggttatgccc acagcatcag cggcagcagc
     8701 gctgaaggtt ataacggtca ggccacgctg aatgtgactt tctgacagaa ccatcgcctc
     8761 tttgtggtcc cggtcatcat gatcgggacc cggaccggcg caacggatat tcaacgccac
     8821 attcgctggc attaacaata acatgatatt catcacggag tgactatgtt acagatagtc
     8881 ggcgcgctga tcctgctgat agtaggattt gccattcttc gccttttgtt cagagcatta
     8941 atcagcacgg cttctgcgct ggcagggctt atattgctgt gtctgttcgg cccggcttta
     9001 ctggctggct atatcaccga acgcataacc cggttgttcc atattcgctg gctggcaggc
     9061 gtatttctga cgattgctgg aatgatcatc agcttcatgt ggggacttga tggtaaacat
     9121 atcgcgctgg aggctcacac ctttgactct gtgaaattta ttctgaccac cgctctcgcc
     9181 ggtggtctgc tggctgttcc cctgcagatc aaaaacattc agcagaacgg gatcacacca
     9241 gaagatatca gcaaggaaat taacgggtat tactgctgtt tttatactgc ctttttcctt
     9301 atggcgtgtt ctgcatgcgc accattgatc gcgttacagt acgatatttc accgtcactg
     9361 atgtggtggg gcgggttgtt atactggctg gctgcattag tgacgctgct atgggcggcc
     9421 agccaaatcc aggcgctgaa aaaactgacc tgtgccatca gccagacact ggaagaacaa
     9481 ccggtgctca acagtaaatc gtggctgacc agtttgcaaa acgattacag ccttcctgac
     9541 tcactgacgg agcgcatctg gctgacgctc atttctcaac ggatttcccg gggagagctg
     9601 agggaatttg aactggcaga cggaaactgg ttactgaaca atgcctggta tgaaagaaac
     9661 atggcagggt ttaacgaaca gttgaaagag aacctgtcat tcacacctga tgaactgaaa
     9721 acgctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt tctcgatcgt
     9781 tgcctggacg gcggtgactg gtattccttt tcagaaggtc gccgttttgt atcattccat
     9841 cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca tcatgccccg
     9901 gaaaatcata agccggatcc ggagtggtac tgctcctctc tttgtcgcga aacagaaaca
     9961 ctatgtcagg aaatttatga acgcccttac aactgtttta tttccgatgc aacggcgaat
    10021 ggtctgattc tcatgaaact accggaaacc tggagtacaa atgagaaaat gtttgcttcc
    10081 ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga cagagtccgt
    10141 ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga cagactggtc
    10201 agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt cggtgcggca
    10261 ttcgacggac aaaacggaca gtatcgttac atgggaaata atggccccat gcaactggaa
    10321 gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga ggaataagat ccgcgaaggt
    10381 aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc tgattcgtcg gggacatctg
    10441 acttatactc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt cacttatgat
    10501 atttccgagg gttcagttgt cagtctggcg gccggaggga tcagttttgc cctgacggca
    10561 tcgctcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc tgctgtccag
    10621 gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca actccaccgg
    10681 ctcagtgttg ttcagggtat gctgaagcac attgattttt cgactgccag cccgactgtc
    10741 cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtaatg
    10801 aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc
    10861 aaaatgttgc ggggacggat ctccggtgcg cagttcatca gaaatcttgc cgtggcatct
    10921 tccggtgtgg caggtggtgc tgtcggatca gtggcgggcg gaatactgtt cagtccactg
    10981 ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc
    11041 tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg
    11101 gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac cggacatgag
    11161 attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct ggagatcatt
    11221 ttcgccgccg gtataccaca acgggccgcg accaatatgt taatcaaacc actggtggtc
    11281 agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg caatatggtt
    11341 aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt ctgcgccttt
    11401 tacgttttat cacctgattt gccagtgtca tcgtcatgac tcagagaggt aaataccatg
    11461 attcacctgt tcaaaacctg catgattacc accttcattc tggggttaat gtggtctgcc
    11521 ccactccggg cacaggatca acgttatatc agtatacgca atacagatac gatatggctc
    11581 ccgggaaata tttgtgctta ccagttccgg ctggataatg gcggaaacga tgaaggattt
    11641 ggccccctca ccatcactct gtaactcaaa gacaaatatg gtcagacgct ggtgaccaga
    11701 aaaatggaaa cggaagcttt tggtgacagt aatgccacgc gaaccacaga cgcatttctg
    11761 gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca ttaaagcaac tgaagaaagt
    11821 aacggccatc gtgtcagtct gccgttatcg gttttcgatc cccatgacta ccatccactg
    11881 ctaattaccg tttccggaaa aaacgttaac tgatccctga agtcagtttc atgcttcagc
    11941 ctctgtcaca acgcgtggca gaggcttttt atttatcagg agaaaacaga tatgccgggt
    12001 tacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt tgctgtcata
    12061 acagcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg ttgggttatc
    12121 cgcaacgcgg aaggtcagcg acgctggcgc tgccggaatt ctgaacccga tgccggaaag
    12181 gtgcttaata cgcggctcgc cagtgatgct cttctcagac aataaacgtc attcatttca
    12241 cccatcaggc cgcgtcttct ccgggagacg tggctttttc atttattaca ccagtaaatc
    12301 ttaaccaccg ataaggagca aattatgcga ctagccagtc gttttggtta tgcgaaccag
    12361 atacgccgtg accgtccgct gacacacgaa gaactgatgc actatgttcc cagtattttc
    12421 ggtgaagacc ggcatacttc ccgcagtaaa cgatatgcgt acattcccac tatcaccgta
    12481 ctggaaagcc tgcagcagga aggttttcag ccattcttcg cctgccagat ccgcgtgcgc
    12541 gacccgggcc gccggggata cacaaaacac atgctgcgtc tgcggcgggc cggagagata
    12601 aacggagaac atgtccctga aattattctg ctcaactctc atgacggtac ctccagctac
    12661 cagatgctgc cgggttactt caggttcgtc tgccagaacg gatgcgtctg tggtcagtct
    12721 ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg acagagtcat tgaaggggct
    12781 tacgaggtgg tgagcgtgtt tgaccggata gaggagaagc gtgatgccat gcagtcgctg
    12841 ctcttaccgc caccggcacg ccaggcgctg gcacaggcgg cactgactta ccgttatggt
    12901 gacgaacatc agcccatcac cactgccgac attctgacac cacgacgccg ggaggattac
    12961 ggtaaggacc tgtggagtgc ttatcagacc attcaggagg atatgctgaa aggcgggatt
    13021 tccggtcgca gtgcaaaagg aaaacgtatc cacacccgtg ccattcacag catcgacacc
    13081 gatattaagc tcaaccgcgc attgtgggtg atggcagaaa cgctgctgga gagcatgcgc
    13141 tgatgaaaca tatccctgaa ggcgtgattc aggcaccaga atcacccgac gccccggagg
    13201 cgctgccggg gtgtcgttcc atttctgatg atgacctgtg cgcctggtgt acccgtctgc
    13261 tttaccgtcc cggagaactc agtctgtgca gactgagtac aggcaatggc atctggccat
    13321 ccgtctgtga ccggaacggt tacgcgtacg gctgtccgga atttcagccg aatatcattc
    13381 atccctgata ttaccgggtc tcacggtcag tgatgaacgt gatgttgcca gctcccgacc
    13441 tttactccct ctcatttatt cacattacaa ggatttcata catgaaaaca ttgtctcaga
    13501 acaccacctc ttcggcctgt gcaccggaga ctggcctgca acaactggtt gccaccatag
    13561 tccctgatga acagcgcatc agcttctggc cgcagcattt tggcctcatt ccacagtggg
    13621 tgaccctgga gccccgtgtc ttcggctgga tggaccgtct gtgcgaaaac tactgcgggg
    13681 gcatctggaa tctgtacacc ctgaacaacg gtggcacatt tatggcacct gaaccggatg
    13741 acgataatga cgaaacatgg gtactgttca atgtcatgaa cggtaaccgc gctgaaatga
    13801 gcccggaagc cgccggcatt gctgcctgtc tgatgacgta cagtcatcat gcctgtcgta
    13861 cggagaatta tgccatgacg gtccattatt accggttgcg ggattacgcc ctgcagcatc
    13921 cggaatgcag cgccattatg cgcatcattg actgaaaggg gccggaataa tgcaacagct
    13981 ttcctttctg cccggagaaa tgacgcccgg cgagcgcagt ctcattctgc gggccctgaa
    14041 aactctggac cgccatcttc atgaacccgg cgtggccttc acctccaccc gtgcagcacg
    14101 ggaatggctg attcttaaca tggcgggact ggagcgtgaa gagttccagg tgctgtatct
    14161 gaacaaccag aatcagctga ttgccggtga aaccctcttc accggcacca tcaaccgcac
    14221 ggaagttcat ccccgggagg tgattaaacg cgccctgcac cacaatgccg ctgccgtggt
    14281 actggcgcac aatcacccgt ccggtgaagt cacccccagt aaggctgacc ggcttattac
    14341 tgaacgtctg gtacaggcgc tggccctggt ggacatccgg gtaccggacc atctgatagt
    14401 tggtggcaac caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac
    14461 atcacacctg ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaattatc
    14521 acccgtggtg aagccatgcg tattcaccaa caacatccga catcccgtct ttttccgttc
    14581 tgtaccggta aataccgctg gcacggcagt gctgaagcgt ataccggtcg tgaagtgcag
    14641 gatattcccg gtgtgctggc cgtgtttgct gaacgccgta aggacagttt tggcccgtat
    14701 gtccgtctga tgagcgtcac cctgaactga atcagaaagg ggattctgat gagcagaatt
    14761 atcaccacca ccgtgtatac gctgaatgaa ctttcctgcc cggcacggga aaaagcccgc
    14821 gactggtacc gtcagcatca tgctgacagc aactggtatg aaaacgtgta tgaggacttc
    14881 agggaggtct gtggcatttt cggaattgac ctccgccagc gcgttttccg gctcagcaac
    14941 ggcaggttta tggaagagcc gtgcatctgg ttcagcggat tctgcagcca gggggacgga
    15001 gcctgttttg aagggcgctg gcactggcaa ccagcggcac cccggaaaat cagggagtat
    15061 gccccgcaag accatgaact ccatcgtatc gcggatgccc tgcaggcggt gcagaaacgt
    15121 aacttctggc agttgcaggc tgaaatcagt caccggggac gctactgcca tccgtacagc
    15181 atggatatca cggttacccg gaacagtccg actggtcagg caatgacagc cgatgcggaa
    15241 gccgttgtca gcgaagccct gcgcgacctg gccttctggt tatacagtca actggaaaac
    15301 gagtacgaca gtctgacctc agatgccgct gtggatgagg cccttctcat caacgaatac
    15361 accttcaccg aagccggtct gcgcgccggc tgacctgtca tcgttaaacc attcttaacc
    15421 gattttctgt gaggatttta tcgtgtcaga cacgctccat gaaacaaatt atcccgacga
    15481 caacaacgac cgcccctggt gggggctacc ctgcaccgtg acgccctgtt tcggggcacg
    15541 tctggtgcag gaagggaacc ggttgcatta ccttgcagac cgcgccggta tcagaggccg
    15601 gttcagggac gcagatgcat accacctgga ccaggccttt ccgctgctga tgaaacaact
    15661 ggaattaatg ctcaccagcg gtgaactgaa tccccgccat cagcataccg tcacgctgta
    15721 tgccagaggg ctgacctgca aagccgacac cctcggcagt tgtggttacg tttatctggc
    15781 tgtttatcca acatccgaaa cgaaaaagta actctccaga ataaccttct gctccggtct
    15841 ggtgttttca ccacgccact tttccatttt tatctctgca tatcaggaaa atcttcagta
    15901 tgaaaacatt acctgacaca cacgtacggg aggtatcgcg ctgcccgtct cccgtcacca
    15961 tctggcagac actgctcacc cgactgctgg accagcacta cggccttacg ctgaatgaca
    16021 caccgttcgc tgatgaacgt gtgattgagc agcatattga ggcaggcatt gcactgtgcg
    16081 atgcggtgaa ttttctcgtt gaaaatacgc actggtgcgt accgaccagc cgggattcag
    16141 cgcctgtacc cgctctcagt taataaacag catcgatatc ctccgggccc gccgggcaac
    16201 tggcctgatg acccgcggca attacaaaaa ggtaaataac attaccctgg gtaagtatca
    16261 ggaggcgaaa cgatgaaact ggccctgaca ctggaagccg acagcgttaa cgtgcaggca
    16321 ctgaacatgg ggcgaattgt cgttgacatt gatggtgttg agctcactga actgattaac
    16381 aaggtcgctg aatacggtta ttcactccgc gtggtggacg cccctgacca gcagtcaacc
    16441 tgcactccac caccgtttac gacattgcgt ggctgcagga caatatcgac tgcgaatcgg
    16501 agattatctt tgacaacaat gaggataaaa cggattcggc agcattgttg ccctgtatcg
    16561 aacaggccag ggaagatgtc cgtaccctgc gtcacctgca actcattcac ctgagcgcgt
    16621 gagtctcact caccatactt cattccactg acgccagcct gaacagggct ggcgttttca
    16681 tttatctgca aaaaggaaaa acgattatgt caggtatcac aatctcccgt cctgaagtgg
    16741 tcaacgggca tacggacgtt atctgttcca cctcaatcgg acacattctg gctgtacgaa
    16801 agaccacact gatgcaaatc gacacactta tccggcaact gacagaaatc tcagcaatga
    16861 cagaaagtat tggagataaa accgccccgg actaggccat gaaacaggat tttcgctgtg
    16921 gttgctggct gactgagaaa ccgcaatgaa agccattacc cgcaaccttg accgtgaaat
    16981 ctggcgtgac ctgatgcaac gttccgggat gctgtcatta atggatgcac aggcccgtga
    17041 tacgtggtac cggtcactgg agtacgataa ttctccggaa atcagcgaag cgaacattct
    17101 gagcacattt gaacaactgc accagaacaa ggatgaggtg tttaagcggg gagtgattaa
    17161 cgtcttcagg gaactgagct ggaattataa aaccaactct ccctgtaaat ttggaagtaa
    17221 aatcattatc aacaatctgg cgagatggga ccggtggaga tttcatctta tcaccggaca
    17281 acagactgac cgactcgcct acctggaaag aatgctacat ctgttcagcg gcaaaccgat
    17341 ccccgacaac cgggaaaaca tcactattcg tctggatggt cacatccagt ccgttcaggg
    17401 taaagagcgc tatgaagacg agatgttcat cattaaatat tttaagaagg gatctgcaca
    17461 catcactttc aaaaggctgg aactgattga cagaattaac gatataatag ccaagcactt
    17521 tctttctgtg ctctcagcct gatcccgagt gtgattccct ttcaatatca aaagggactg
    17581 cgggtacaaa tgagggtaca tttccaatca aaccaaataa atcacatcaa tatcaatatg
    17641 ataaataaaa tcttcgattc cgagtccggc accactattc taaagaaccc gcccacaagg
    17701 cgggtttttg cttttggatc tttatataaa ataaacttgg gagcataacg ccatcaggtc
    17761 ttaaacactc ccgaatcatc aataaacatc ccccgttaac aaaacacagt tcagttcaca
    17821 taacatccct gttatcccac acatatctgc tggccgaacg acgccagtgc gcgtacggag
    17881 gcaagagcag ccctgcacat tgcagcgctt gtggtaacct ggtttaatcc ggtgataaag
    17941 gcgttttaca tgcatctgcc agcagccgga aaaaccaaaa aagtggcgct ggttgactgt
    18001 atgcgtaaat tgctgaccat cctgaacgca atgctcagaa agaacgaaga gtgaaatgaa
    18061 tcgtagcatc atgttgctcc ataattttta cgttcaagac agttgattat cggcgcatat
    18121 gactcaaaaa gagcctgatg attgcctctt ttccataggc tctttctctg aagtgaagga
    18181 aggcccggaa gaaagcactc gctggcatac gtgactccag ctcaaccaga gataatcacc
    18241 attgttattt ttctatttaa taataatccc ctgaaaaaca gatatagcaa aaatacaatc
    18301 cggccagatt taattccgca ttcattacca gaaataa
//
LOCUS       DAJOTB010000095        18023 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJOTB010000095 DAJOTB010000000
VERSION     DAJOTB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.095"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(340..1218)
                     /locus_tag="OHN28_003191"
     CDS             complement(340..1218)
                     /locus_tag="OHN28_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCQ2298532.1"
                     /translation="MTSDKTIKQAISNITIWRKGEQRAPHKPLLLLYVLSLYRQGHGR
                     LFNYGSEIYEPLLDLLERYGPQRRDQRPDMPFWRLKGDGFWELQNAELCSTSGSRQPP
                     RRELIEYNVAGGFDADNFALVAKNHRLIDTLAQQLLGAHFPASIQEEIADEMGFDIRT
                     SLRQRDPKFRQMVLRAYNYQCAICGFNMRHDNAPIALEAAHIRWKQHHGPCEVPNGLA
                     LCAIHHKAFDRGSIGLDENMRVVVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGE
                     QFVEWHRKEVFRGEGI"
     gene            complement(1434..2702)
                     /locus_tag="OHN28_003192"
     CDS             complement(1434..2702)
                     /locus_tag="OHN28_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCQ2298533.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEDHANRVKKSLEDNIFPAIGARNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLEKID
                     SYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     SKMHTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANRDRAITPFDYAKINRGNGK"
     gene            complement(2903..2987)
                     /locus_tag="OHN28_003193"
     tRNA            complement(2903..2987)
                     /locus_tag="OHN28_003193"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2951..2953),aa:Leu,seq:caa)
     gene            3183..4202
                     /gene="ahr"
                     /locus_tag="OHN28_003194"
     CDS             3183..4202
                     /gene="ahr"
                     /locus_tag="OHN28_003194"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCQ2298534.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMHFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            4332..5834
                     /locus_tag="OHN28_003195"
     CDS             4332..5834
                     /locus_tag="OHN28_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCQ2298535.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIHRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRPNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5995..7077)
                     /gene="lptG"
                     /locus_tag="OHN28_003196"
     CDS             complement(5995..7077)
                     /gene="lptG"
                     /locus_tag="OHN28_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2298536.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNDNRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7077..8177)
                     /gene="lptF"
                     /locus_tag="OHN28_003197"
     CDS             complement(7077..8177)
                     /gene="lptF"
                     /locus_tag="OHN28_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2298537.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8444..9955
                     /gene="pepA"
                     /locus_tag="OHN28_003198"
     CDS             8444..9955
                     /gene="pepA"
                     /locus_tag="OHN28_003198"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCQ2298538.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10213..10656
                     /gene="holC"
                     /locus_tag="OHN28_003199"
     CDS             10213..10656
                     /gene="holC"
                     /locus_tag="OHN28_003199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCQ2298539.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10656..13511
                     /gene="valS"
                     /locus_tag="OHN28_003200"
     CDS             10656..13511
                     /gene="valS"
                     /locus_tag="OHN28_003200"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCQ2298540.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13558..14745)
                     /locus_tag="OHN28_003201"
     CDS             complement(13558..14745)
                     /locus_tag="OHN28_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCQ2298541.1"
                     /translation="MNNVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPLFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRSLFLNNLTLANGAIRFHSSVTAIGMLLRMLALLMG
                     ASITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            14938..15441
                     /locus_tag="OHN28_003202"
     CDS             14938..15441
                     /locus_tag="OHN28_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2298542.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            complement(15618..16034)
                     /gene="rraB"
                     /locus_tag="OHN28_003203"
     CDS             complement(15618..16034)
                     /gene="rraB"
                     /locus_tag="OHN28_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCQ2298543.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            16196..17200
                     /gene="argF"
                     /locus_tag="OHN28_003204"
     CDS             16196..17200
                     /gene="argF"
                     /locus_tag="OHN28_003204"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCQ2298544.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAASLTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            complement(17245..17697)
                     /locus_tag="OHN28_003205"
     CDS             complement(17245..17697)
                     /locus_tag="OHN28_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2298545.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKA"
BASE COUNT         4637 a         4613 c         4537 g         4236 t
ORIGIN      
        1 aatatttctt tccagaatag tgagcttagt cccttgctta aaaaacaaaa caaatttctt
       61 caacctaaga aaaacaatga atttattatt taactccatc ttatttacaa aatttgaaat
      121 tcttatatag ctatacaacg cataaataaa tacagcaagt acagtaaatg ccgacagatg
      181 aaactgttta ataaacgacc acccgcgaaa taaagacaga atagaaataa aaataaagaa
      241 agacaaccat ttcacattac gcctaaccat ataccctcca cataataaag ccgttatcaa
      301 taactaactt aataatctaa agaaaaaaat caatctattt cagattccct ctcctctgaa
      361 cacctctttt ctgtgccatt caacaaactg ctcgcccgga taattttctt taaccggtgg
      421 caacgcaatc gcctttcccg caaaatccca gaacagtcgc tgtaccactc caccaccatt
      481 taccgcaccg gaaaccacca cacgcatatt ttcatccagg ccaatcgaac cacgatcaaa
      541 cgctttatgg tggattgcac acaatgccag gccattaggt acttcacaag ggccatggtg
      601 ctgcttccat ctgatatgag cggcttccag agcaatcggc gcattgtcgt ggcgcatgtt
      661 aaagccgcag atagcgcact gataattgta agcacgtagc accatctggc gaaacttcgg
      721 atcgcgttga cggagagagg tgcgaatatc aaaacccatc tcgtcagcga tctcttcctg
      781 aatactggcc gggaaatgcg cccccaggag ctgctgcgcc agcgtatcaa ttaacctgtg
      841 attttttgcc accaacgcaa aattatcggc atcaaatcca ccagctacat tatattcaat
      901 gagttcacgt cggggcggct ggcggctacc actggttgag cacaattccg cgttttgtag
      961 ttcccagaag ccatcaccct tcaaacgcca gaacggcatg tccgggcgct ggtcacggcg
     1021 ctgcggtcca taacgttcca gaagatccaa caggggctcg tagatttcag aaccatagtt
     1081 aaacaggcga ccatgaccct gccgatagag tgagagaacg tacagcagca ataatggctt
     1141 atgtggcgca cgttgttcgc ctttgcgcca gatggtgata tttgatatcg cttgttttat
     1201 ggttttgtca gaggtcattg cgttggagaa tccatgagca catatttgcg atgatgctcg
     1261 caactatatg tacattcaag gcattattgc agacatcctt tttctctggc ggttctgtac
     1321 ctggcagata gaattctgat aacaacggta aaaaatacag cgaccaacca gtcctaagcg
     1381 ggctggttgg tcttttaaat ttaaacatca gccgaggaat aacggcattc aactcacttg
     1441 ccattacccc gattgatctt cgcatagtca aacggcgtaa tcgctctatc ccgattggca
     1501 tccagaaaat ccgcccacca ctgcaacatc agcttgcgct catccagatg ttccgcttta
     1561 tggatatacg ccgccctcac cgaattgcgt tccatatggc tcatctgcct ttccaccgca
     1621 tccttcgacc atagccctga ctcaattaac gaactacagg ccattgtgcg gaagccgtga
     1681 ccacaaacct caaccttagt gtcatacccc atcacccgta acgcactgtt taccgtgttc
     1741 tcgctcatgg gcttacgcgg atcatgatcg ccaataaaaa tcagctcatg ttcgccacag
     1801 aactgtttta tctgcttcag gatcgccagc gcctgctttg aaagcggcac cagatgcggt
     1861 gtatgcatct ttgatcctcg ctgagaatgt ttcacgccgg gaataggctc tcgctcaggc
     1921 gggatggtcc acattgacgt ttcaaaatcg atctctgacc agcgagcaaa acgcagctca
     1981 ctggaccgaa taaagataag cagagtaagt tccgttgccc agcgagttag cggcctgccg
     2041 gtatagctgt ctattttctc aagtaactca gggatgcgct ttaactccag cgccggacga
     2101 tgctgtcgat tactggaagc aaccgccccc gccatctctt gcgccggatt gtaatcaatt
     2161 aacccgcttt gcactgcata acgcatgatg gccgtggtgc gctgttgaag gcgggaagcc
     2221 acttcaagac gtccggattt ctccacggct ttaataggaa ttaacagatc gcgggtgccc
     2281 agttcagcaa tattgcgagc accaattgcc gggaagatat tgtcctccag gctctttttt
     2341 acacgattag catgatcttc cgaccatttt tggttggtta cgagccactc tctggcaacc
     2401 ttctcgaaag taataatctc tttcgcctgc tcttctttca cagcacgctt atgctcacga
     2461 ggatcgatac cagcagctac cagctttctg gcctcttccc ttttcttccg cgcatccgcc
     2521 agtgaaacat ccgggtaaac gccgaacgcc atcaaatgtt gtttaccgcc aaagcggaaa
     2581 cgaaatcgcc aatactttga accgccaggt tttacaagta aggacatgcc gtcgccgtca
     2641 acaagtgaat actctttctc ctctggtttc gcagaacgaa ctttagtatc cgttagggcc
     2701 ataatggtct tccttccatt ggtatctcgt gagtatacaa gcaatatcga atcggcaaat
     2761 atactcatta gtatactcac aagcctgttg atgtgggttg agtcaggttg acttcagttg
     2821 aggagaaaag ctggaaaagc cttgcgtggc gcggatttca ggcataaaaa aagacctcag
     2881 ttgaggtcta tttacatact tttggtgccg aaggccggac tcgaaccggc acgtatttct
     2941 acggttgatt ttgaatcaac tgcgtctacc gatttcgcca cttcggcact gaagaggtat
     3001 gcggaaaacg atgtggatta tacctgtcgc acgccaccat gcaagcgcca gcttgctcgc
     3061 atctcgctaa gtgctgaaat tttcagcatt gcatacagcg atgtgtaacc tttgtcacac
     3121 tccaggcacc ccgccctgcc atgctctaca cttcccagac aacaccagag aaggacaaaa
     3181 atatgtcgat gataaaaagc tatgccgcaa aagaggctgg cggcgaactg gaagtttatg
     3241 agtacgatcc cggtgagctg aagccacaag atgttgaagt gcaggtggat tactgcggga
     3301 tctgccattc cgatctgtcg atgatcgaca acgaatgggg attttcacaa tatccgctgg
     3361 ttgccgggca tgaggtgatt ggtcgcgtgg tggcgctcgg gagcgccgcg caggataaag
     3421 gtttgcaggt cggtcagcgt gtcgggattg gctggacggc gcgtagctgt ggtcactgcg
     3481 atgcctgtat tagcggtaat cagatcaact gcgagcaagg cgcggtgcca acaattatga
     3541 atcgcggtgg ctttgccgag aagctgcgtg cagactggca atgggtgatc ccactgccag
     3601 aaaatattga tatcgaatct gccgggccgc tattgtgcgg cggtatcacg gtctttaagc
     3661 cactgttaat gcaccatatc accgccacca gccgcgtcgg ggtcattggt ataggcgggc
     3721 tggggcatat cgctataaaa cttctgcacg caatgggatg cgaggtgaca gcctttagtt
     3781 caaatccggc gaaagagcag gaagtactgg cgatgggtgc cgataaagtg gtgaatagcc
     3841 gcgatccgca ggcactgaaa actctggcgg ggcagtttga tctcattatc aatactgtga
     3901 acgtcagcct cgactggcag ccttattttg aggcgctgac gtacggcggt aatttccata
     3961 cggtcggtgc agttctcacg ccgctgtctg ttccggcctt tacgttaatt gcgggcgacc
     4021 gcagcgtctc tggttcagca accggtacac cttatgagct gcgtaagctg atgcactttg
     4081 ccgcccgcag caaggttgcg ccgaccaccg aactgttccc aatgtcgaaa attaacgacg
     4141 ccattcagca tgtacgcgat ggtaaagcgc gctaccgagt ggtcctgaaa gccgacttct
     4201 gatccctttg ccgggtgcct gtcgcccggc ttttaaatct ctttggaagg cgtctcgcaa
     4261 tctgctgaat ttagcggtga agagtgcaca gccgttgcct atactccagg caggaaactg
     4321 gaggaaatct catgagcgaa cccctgttaa ttgcccgcac gccggacaca gaactgtttt
     4381 tactgccggg aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa
     4441 ctgtcacgtt gcaaaaactg gcagagtcat tgtcggaaat cggtgttccg gtgtttatgg
     4501 ccgatgtgaa aggcgatctg accggcatcg cgcaggcagg aacggcgtcg gaaaaactgc
     4561 tcgcaagact gaaaaatatc ggcgtcaacg actggcaacc gcatgccaat ccggtggtgg
     4621 tgtgggatat ctttggcgag aaaggccatc cggtgcgggc gacggtttcg gatctggggc
     4681 cgctgttgct ggcacgactg ttgaatctca acgatgtgca atctggcgtg ctgaatatca
     4741 ttttccgcat tgctgacgat cagggattgt tgctgctcga ctttaaagat ctgcgggcta
     4801 ttacccagta catcggcgat aacgccaaat ccttccagaa tcagtacggt aatatcagta
     4861 gtgcatcggt tggtgccatc catcgcgggc tgttgtcgct ggaacagcaa ggcgcagcac
     4921 acttctttgg tgagccgatg ctggatatca aagactggat gcgcaccgat gccaacggta
     4981 aaggcgttat caatatcctt agcgccgaaa aactctacca gatgccgaaa ctgtacgccg
     5041 ccagcctgtt gtggatgctt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg
     5101 agaaaccaaa actggtgttt ttcttcgacg aagcgcatct gctgtttaac gatgcaccgc
     5161 aggtactgct ggataagatt gagcaggtga taaggcttat tcgctcaaaa ggcgtgggcg
     5221 tctggttcgt ttcgcaaaac ccgtcagata tcccggataa cgtgctcggg cagctcggta
     5281 atcgcgttca acacgctttg cgtgctttta cgcctaaaga tcagaaagca gtaaaagctg
     5341 cggcgcaaac catgcgcccc aaccctgcat ttgatacgga aaaggcgatt caggaactag
     5401 gaaccggcga agcgttaatc tcgtttctcg atgcgaaagg aagcccttca gtggtggaac
     5461 gggcgatggt gattgccccc tgttcacgaa tggggccggt gacggaagat gagcgcaatg
     5521 gcttgattaa ccactccccg gtgtatggca agtacgagga tgacgtggac cgggaatccg
     5581 cctatgagat gttgcaaaaa ggctttcagg ccagtatcga gcagcaaaat aatcctcccg
     5641 cgaaagggaa agaggtggcg gtggatgacg gtattctcgg tggattgaag gatattttgt
     5701 ttggcactac tggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggcaaaaa
     5761 gcgccgcccg ccaggtgacg aatcagattg tgcgcgggat gttggggagt ttgctgggag
     5821 ggagaagaag gtaagccagg tgcgatttgt ctttgcctga tgcgacgctg gcgcgcctta
     5881 tcatgccaac accactgcaa tatattgaat tttcatgatt tttcagacat gaacgaagca
     5941 cactttgcta acaataataa agggagcttt cgctcccttt attcgttcat caggttacga
     6001 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
     6061 gatgatcggc gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta
     6121 gaagacaaaa ccgaagctaa taccagtgac cacacgcacg cccatcggta cgctacgcag
     6181 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
     6241 gattttgctc cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata
     6301 cttcacatag ttgtgcaaac cgctgataga gagtgcatcc gggtccagcg ccaccacgcc
     6361 cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtgat
     6421 ctgtttcgga ttggtcaaat cagattcatc aacctgcgac agacgccaga ctttatgttc
     6481 cgggtcaaac ttcgcggtag cggcatagcg tacggattgc agacgacgat tatcgttaaa
     6541 ggcataaata ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtagacgaa
     6601 gttgttgcca tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat
     6661 cgcctgcgca cggtagttac gcgccatctg ctcgccctgt ggcgcgaccc attcaccaat
     6721 cgccatcgtc agcaagacca gcggaatggc ggttttcatc accgacagcg ccacctgcat
     6781 acgggtaaaa ccagaagcct gcatgactac cagttcgctg cgctgcgcca gcatcccaag
     6841 accaagcaac gccccaagca gagccgccat cgggaagaag atttgcacat ctttcggcac
     6901 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     6961 cagctgatcg acaaacttga taatgcccga cagcgacacc agcatgaaca gtgtcatcat
     7021 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     7081 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     7141 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg
     7201 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaacaacaga
     7261 tacagcagca tggctggcag catcgatagt acgcgtccct gacgcgggtt aaccacgctc
     7321 agcggtacga ccataagtgc catcataaac acggtgaaca ccaacgtgat acgccagttc
     7381 agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg
     7441 gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa
     7501 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
     7561 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
     7621 gaaggtcgtg cattaccttt tggtcgaatt tgcgcgagga acacatcttt gaaatcgctg
     7681 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
     7741 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
     7801 cgcgatgacc atggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaacacc
     7861 gcaaggatca ttgccgcttt caccagcacc gctttgctca gaccgcaggc atgcattacc
     7921 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
     7981 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
     8041 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
     8101 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
     8161 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
     8221 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
     8281 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
     8341 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
     8401 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
     8461 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
     8521 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
     8581 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ctgttgctgc accatgtgcc
     8641 aaacgtgctt tctgaacgca tcctccttat tggttgcggc aaagaacgtg agctggatga
     8701 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat
     8761 ggaagcggtc tgctttctga ccgagctgca cgttaaaggc cgtaacaact actggaaagt
     8821 gcgtcaggct gtcgagacgg caaaagagac gctctacagt ttcgatcagt tgaaaacgaa
     8881 caagagcgag ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
     8941 gctgaccagc ggtgaacgcg cgatccagca tggtctggcg attgccgcag ggattaaagc
     9001 cgcgaaagat ctcggcaata tgccaccgaa tatctgtaac gctgcttacc tcgcctctca
     9061 ggcgcgccag ttggctgaca gctacagcaa gaatgtcatc acccgagtta tcggcgaaca
     9121 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcaaaacga
     9181 atcgctgatg tcagtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
     9241 gctggtgggt aaaggtttaa ccttcgactc cggcggtata tccatcaagc cttcagaagg
     9301 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggtg tgatgcggat
     9361 ggtcgcggag ctgcaattgc cgattaacgt tattggcgtg ctggcaggct gcgaaaacat
     9421 gcctggcggg cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt
     9481 tgaagtgctg aacaccgatg ccgaaggccg cctggtactg tgcgatgtgt taacttacgt
     9541 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgttat
     9601 cgcgctaggc catcacatta ccggtctgat ggcgaaccac aatccgctgg cccatgaact
     9661 gattgccgca tctgaacaat ccggtgaccg cgcatggcgt ttaccgctgg gtgacgagta
     9721 tcaggaacaa ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
     9781 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcatct
     9841 ggatatcgcg ggaaccgcct ggcgctctgg taaagcgaaa ggcgccaccg ggcgtccggt
     9901 ggcgttgctg gcacagttcc tgctgaatcg cgccgggttt aacggcgaag agtaattgca
     9961 tcaggtaaag ttgtctttgt cggatgcggc gtgaacgcct tatccgacct acacagcact
    10021 gaactcgtag gcctgataag acgcggcagc gtcgcatcag gcaaggctgt tattgtcgga
    10081 tgcgacgtga acgccttatc cgacctacac acagcactta acttgtaggc ctgataagac
    10141 gcggcagcgt cgcatcaggc gctgcggtgt atacctgatg cgtatttaaa tccaccacaa
    10201 gaagccccat atatgaaaaa cgcaacgttc taccttctgg acaatgacac caccgtcgat
    10261 ggcttaagcg ccgttgagca gttggtgtgt gaaattgccg cagaacgttg gcgcagtggt
    10321 aagcgcgtgc tcatcgcctg tgaagatgaa aagcaggctt accgactgga tgaagccctg
    10381 tgggcgcgtc cggcagaaag ctttgttccg cataatttag cgggagaagg accgcgcggc
    10441 ggtgcgccgg tggagatcgc ctggccgcaa aagcgcagca gcagcccgcg cgatatattg
    10501 attagtctgc gaacaagctt tgcagatttt gccaccgctt tcacagaagt ggtagacttc
    10561 gttccttatg aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg
    10621 gctggtttca acctgaatac ggcaacctgg aaataatgga aaagacatat aacccacaag
    10681 atatcgaaca gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg
    10741 atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc
    10801 atatgggtca cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc
    10861 agggcaaaaa caccctgtgg caggtcggta ctgaccacgc cgggatcgct acccagatgg
    10921 tcgttgagcg caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag
    10981 ctttcatcga caaaatctgg gaatggaaag cggaatccgg cggcaccatt acccgtcaga
    11041 tgcgccgtct cggcaactcc gtcgactggg agcgtgaacg cttcaccatg gacgaaggcc
    11101 tgtccaatgc ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg
    11161 gcaaacgcct ggtaaactgg gacccgaaac tgcgcaccgc tatctccgac ctggaagtgg
    11221 aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggcgcga
    11281 aaaccgcaga cggtaaagat tatctggtgg ttgcgactac ccgtccggaa accctgctgg
    11341 gcgatactgg cgtggccgtt aacccggaag atccgcgtta taaagatctg attggcaaat
    11401 atgtcattct gccgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca
    11461 tggaaaaagg caccggctgc gtgaagatca ccccagcgca cgactttaac gactatgaag
    11521 tcggtaaacg tcacgccctg ccgatgatca acatcctgac ctttgacggc gatatccgtg
    11581 aaagcgccca ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgaaatcc
    11641 cggcagagtt ccagaaactg gagcgttttg ctgcacgtaa agcagtcgtt gctgcggttg
    11701 acgcgctcgg cctgctggaa gaaattaaac cgcacgacct gaccgttcct tatggcgacc
    11761 gtggtggcgt ggttatcgaa ccgatgctga ccgaccagtg gtacgtacgt gccgacgtgc
    11821 tggcgaaacc ggcagttgaa gcggttgaga atggcgacat tcagttcgta ccgaagcagt
    11881 acgaaaacat gtatttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagc
    11941 tatggtgggg tcaccgtatc ccggcatggt atgacgaagc gggtaatgtt tatgttggtc
    12001 gcaacgaaga cgaagtgcgt aaagaaaata acctcggtgc tgatgttgcg ctgcgtcagg
    12061 atgaagacgt tctcgacacc tggttctcct ccgcgctgtg gaccttctcc acgctcggct
    12121 ggccggaaaa caccgacgca ctgcgccagt tccacccaac cagcgtgatg gtatctggtt
    12181 ttgacatcat cttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag
    12241 atgaaaatgg caaaccgcag gtgccgttcc acaccgttta catgaccggc ctgattcgtg
    12301 atgacgaagg ccagaagatg tccaaatcca aaggtaacgt gattgaccca ctggatatgg
    12361 ttgacggtat ttcgctgccg gaactgctgg aaaaacgtac cggcaatatg atgcagccgc
    12421 agctggcgga caaaatccgt aagcgcaccg agaagcagtt cccgaacggc attgagccgc
    12481 acggtactga cgcgctgcgc ttcaccctgg cggcgctggc ttctaccggt cgtgacatca
    12541 actgggatat gaagcgtctg gaaggctacc gtaacttctg taacaagctg tggaacgcca
    12601 gccgttttgt gctgatgaac acggaaggtc aggattgcgg cttcaacggc ggtgaaatga
    12661 cgctgtcgct ggcggatcgc tggattctgg cggagttcaa ccagaccatc aaagcgtacc
    12721 gcgaagcgct ggacagcttc cgcttcgata tcgccgcagg cattctgtat gagttcacct
    12781 ggaaccagtt ctgcgactgg tatctggagc tgaccaagcc ggtaatgaac ggtggaacag
    12841 aagccgaact gcgcggtact cgccatacgc tggtgactgt actggaaggt ctgctgcgcc
    12901 tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt
    12961 gcggtatcac tgccgacacc atcatgctgc agccgttccc gcagtacgat gcgtctcagg
    13021 ttgatgaagc cgcactggcc gacaccgagt ggctgaaaca ggcgatcgtt gctgtacgta
    13081 acatccgtgc agaaatgaac atcgcaccgg gtaaaccgct ggagctgctg ctgcgtggtt
    13141 gcagcgcgga tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa acgctggcgc
    13201 gtctggaaag catcaccgtg ctgcctgctg atgataaagg tccggtttcc gttacaaaga
    13261 tcgtcgacgg cgcagaactg ctgatcccga tggcaggcct catcaacaaa gaagatgagc
    13321 tggcacgtct ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgt attgagaaca
    13381 agctggcgaa cgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg aaagagcgtg
    13441 agaagctgga aggctacgca gaagcgaaag cgaagctgat tgaacagcag gctgttatcg
    13501 ccgcgctgta atactcatag tgaaaacgaa ggccggagca tgctccggcc tttttattta
    13561 gataaacgga acatacggca taataccgcg agatatccac attaacgaac cgctatcctg
    13621 cggtttgtca tcattggtta actccagcga atcaaggtcg ccctggacat gggtattgtt
    13681 agcaatccag ctaaccatcc acattttcag ccagggataa gccagaccac aggtaattga
    13741 ggcccccatt aataacgcca acatgcgcag aagcataccg atcgcggtaa cagatgaatg
    13801 aaaacgaata gcgccatttg ccagtgtcag attattcagg aacaaactgc gtaacgtgac
    13861 atagagataa ctggcaaaca caaggatcgc gacaaaatac aggaaataag aagccataat
    13921 ctgcgggtaa tattgtaata caaactcact gccagcatca gacctaccca acatggtcat
    13981 catcattaac tgctgaaata gtggtgcgat cataatacca atcacaatga tgaaaggaac
    14041 catggttaat attgcgagca tgcaaccttt aatgcattct tttacgttta cctgaataga
    14101 aaacttatga atgccaaaag ttgcattatt acccagcaat ggcatccatt tactataggt
    14161 tataccatgg acaacaccta gcccgatggc agaaagcaaa cttaatgcga ctaagttaaa
    14221 gaataaccct cctatcgatg tcgtcacttg tgcaataaga tacagtgcga accaaaaaac
    14281 taacgccaac agaacaggca gtcctagcat aacccaccat gcacgcatca ttgagcagtt
    14341 aaagccaaag cgaatgtcat tcagtgaggt catgttcgcc tgatactgca ggctcttcac
    14401 tgccatacaa ggaataccgc agatcaagag agcaaacagg aataaaccga gcgcaaagtg
    14461 ttgacctgca aaacaagaaa tactgagaag ataaaccacc acaataagta acatgctaac
    14521 aaaaatggca ccacctgttc ccttataact aaatggttgc cccttgagtg tcacatgttg
    14581 ataaatataa cgacggcatt ttatcaatgc ccacggtcca tatattccca gagtaataat
    14641 tgtcagtaaa aaattcacaa gacaaataag aaaatactca ccacctttgc ctgtgaaaac
    14701 aaacgagtgc cgagaattat ctttcccgat gtttacatta ttcatgaact atatccctaa
    14761 aataaattcc attcataccg ataataattc cagttaattg atactggttc gcctagaata
    14821 cgctttatct cagagagata tgcttaatat tttctgattt atcttcttct tgcaccacct
    14881 aagcgagggt ggtattaaag tgagctataa atcatacatt tgaaattgag ctatgctatg
    14941 aatgttgtcg cttctcccgc actccgtttg cgcaaactta ccgtcgccga taatcctgct
    15001 atcgcccacg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga taaaggctac
    15061 accgtcgccg atccgaacct tgacgagctg tatcaagtgt atagtcagcc tggccacgca
    15121 tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc gccattaacc
    15181 gggagtgagt cagacatttg cgaactgcaa aagatgtatt ttcttccggc tatccgcggc
    15241 aaagggctgg caaaaaaact ggccttaatg gcaatggagc aggcgcgaga aatgggtttc
    15301 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    15361 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gtcatgtcga ttgtgaagta
    15421 cggatgctac gcaaactttg aaaaatgccc gcgagcttag actcgcgggc attcgaacat
    15481 taaccacgtg ctttttgagt atgctggatg tgacgtaaat gccttatccg ggctacaaat
    15541 tcaccaggtt gcaggaacta cctgggcctg atatgcgtag cgcatcaggt aacgcagcct
    15601 ttgtcgtcaa cgcttaatta gtgacgaata ccgtcatcgt cttcatcgac aaaatcttca
    15661 tcgtcaccgt cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg
    15721 tcaaatttct ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc
    15781 aacgcacact cgctgaggat gtcgcagcaa atcacgatgt caccatcttc aacttccagc
    15841 tcttctggat cggtcacttc gtaaccgagt ttaaacgctt caactgctgc tttttccagg
    15901 gtttccagat cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg
    15961 ccatcttcca gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt
    16021 tccgggtttg ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac
    16081 cactgacatt gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa
    16141 tcatccatat aaattgaatt ttaattcatt gaggcattag ccacaggagg gttctatgtc
    16201 cgggttttat cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag
    16261 cttgttgcag ttagccgcga agctgaaagc cgataagaaa agcggtaaag aagaagccag
    16321 actcactggc aaaaacatcg cgctcatctt cgaaaaagac tcgacccgta cccgatgctc
    16381 tttcgaagtt gccgcatatg accagggtgc tcgcgttact tatctcggcc caagcggcag
    16441 ccagattggt cacaaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga
    16501 cggtattcag tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg
    16561 cgtgccagta tggaacggcc tgaccaatga gttccacccc acgcagctgc tggcggatct
    16621 cctcaccatg caggagcatt tgcccggcaa agcattcaac gaaatgacgc tggtctatgc
    16681 aggtgacgcg cgtaacaaca tgggcaattc gatgctcgaa gctgcgtcgc ttaccggtct
    16741 ggatttgcgt ctggtcgcgc cacaggcatg ctggccggaa gctgcgctgg tcgccgaatg
    16801 cagtgctctg gcacaaaaac acgggggtaa aatcacctta acggaagata tcgcctctgg
    16861 ggttaaaggc gcagacttta tctataccga tgtctgggta tcgatgggtg agcccaaaga
    16921 gaaatgggcg gagcgtattg ccctgctgcg tgactatcag gtgaatagca aaatgatggc
    16981 cctgaccggt aattcgcagg ttaagttcct ccactgtctg cctgcatttc atgatgagca
    17041 aaccacgctg ggtaaaaaaa tggccgcaga atttggtctg tatggtggaa tggaagtcac
    17101 cgatgaagtc tttgaatctc cggcaagtat tgtctttgac caggcagaaa accggatgca
    17161 caccataaaa gctgttatgg tcgctacatt agcaaaataa cgttttgccg ggcggcggca
    17221 ccggtcgccc ccggcagtga tcacttacgc ttttaatagc ttaactactg ctttccgtac
    17281 ctgagcgggg gctcccaccg cacaaagcgg tttgtgaact tcgccaggat agaaaacgac
    17341 aaaatcccct tcattaagaa tcactgtttt ttcatcgatc ccctgcccga taaaagcaat
    17401 atctttatct gccagccagt cagtctctgg tacgcctgct ggttgcgtgc taaatgtcat
    17461 cccttcctgc ccttttaata caatctggat atcgagataa cgagcatgat actcagcacg
    17521 acgcagttca cctggttcag tcgtatcctc agaaatcaga taaaacagac ggtcgccgtc
    17581 aatagcgtgc ttaccttttg ctgtctcatc gctgacatgt gatttgatgt actcaattgc
    17641 ttcgcgaagt tcttccggca gccaggattg taagtgatgg atgttgccaa caatcataat
    17701 atcttccttt attaaaacaa tgtttcactt ctttccttat acgaaaaaaa gccacctctc
    17761 accatagaaa aatcatcagg ttttgccctt gcgcatgttt attgctggag atgttgtact
    17821 gatgttaagc aggagtaatt ttgtatgcag ttttgctgca taaatcttat aacttagcta
    17881 tgtattttgg tcctaatatc ttgttattgc atatgttaaa agaaaactct cagtcagatc
    17941 acagttcatt ttcttagctt ttaatgcccg tgctgtcata gttatcccgt gaaaatagtg
    18001 ctgtgaaaaa taatcacctt atc
//
LOCUS       DAJOTB010000096        17911 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJOTB010000096 DAJOTB010000000
VERSION     DAJOTB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.096"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(127..873)
                     /gene="cutC"
                     /locus_tag="OHN28_003206"
     CDS             complement(127..873)
                     /gene="cutC"
                     /locus_tag="OHN28_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCQ2298546.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(887..1453)
                     /locus_tag="OHN28_003207"
     CDS             complement(887..1453)
                     /locus_tag="OHN28_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2298547.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1669..3402
                     /gene="argS"
                     /locus_tag="OHN28_003208"
     CDS             1669..3402
                     /gene="argS"
                     /locus_tag="OHN28_003208"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCQ2298548.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3847)
                     /gene="flhe"
                     /locus_tag="OHN28_003209"
     CDS             complement(3455..3847)
                     /gene="flhe"
                     /locus_tag="OHN28_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCQ2298549.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(3847..5925)
                     /gene="flhA"
                     /locus_tag="OHN28_003210"
     CDS             complement(3847..5925)
                     /gene="flhA"
                     /locus_tag="OHN28_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCQ2298550.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5918..7066)
                     /gene="flhB"
                     /locus_tag="OHN28_003211"
     CDS             complement(5918..7066)
                     /gene="flhB"
                     /locus_tag="OHN28_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCQ2298551.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7255..7899)
                     /gene="cheZ"
                     /locus_tag="OHN28_003212"
     CDS             complement(7255..7899)
                     /gene="cheZ"
                     /locus_tag="OHN28_003212"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCQ2298552.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIVEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVIDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQGQVDDLLDSLGF"
     gene            complement(7910..8299)
                     /gene="cheY"
                     /locus_tag="OHN28_003213"
     CDS             complement(7910..8299)
                     /gene="cheY"
                     /locus_tag="OHN28_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCQ2298553.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8314..9363)
                     /gene="cheB"
                     /locus_tag="OHN28_003214"
     CDS             complement(8314..9363)
                     /gene="cheB"
                     /locus_tag="OHN28_003214"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCQ2298554.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9366..10226)
                     /gene="cheR"
                     /locus_tag="OHN28_003215"
     CDS             complement(9366..10226)
                     /gene="cheR"
                     /locus_tag="OHN28_003215"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCQ2298555.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10341..10471)
                     /locus_tag="OHN28_003216"
                     /pseudo
     CDS             complement(10341..10471)
                     /locus_tag="OHN28_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10517..12178)
                     /gene="tar"
                     /locus_tag="OHN28_003217"
     CDS             complement(10517..12178)
                     /gene="tar"
                     /locus_tag="OHN28_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCQ2298556.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQSELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLAAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLAATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12323..12826)
                     /gene="cheW"
                     /locus_tag="OHN28_003218"
     CDS             complement(12323..12826)
                     /gene="cheW"
                     /locus_tag="OHN28_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCQ2298557.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12847..14811)
                     /gene="cheA"
                     /locus_tag="OHN28_003219"
     CDS             complement(12847..14811)
                     /gene="cheA"
                     /locus_tag="OHN28_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCQ2298558.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTSPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14816..15742)
                     /gene="motB"
                     /locus_tag="OHN28_003220"
     CDS             complement(14816..15742)
                     /gene="motB"
                     /locus_tag="OHN28_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCQ2298559.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15739..16626)
                     /gene="motA"
                     /locus_tag="OHN28_003221"
     CDS             complement(15739..16626)
                     /gene="motA"
                     /locus_tag="OHN28_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCQ2298560.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16753..17331)
                     /gene="flhC"
                     /locus_tag="OHN28_003222"
     CDS             complement(16753..17331)
                     /gene="flhC"
                     /locus_tag="OHN28_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCQ2298561.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17334..17684)
                     /gene="flhD"
                     /locus_tag="OHN28_003223"
     CDS             complement(17334..17684)
                     /gene="flhD"
                     /locus_tag="OHN28_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCQ2298562.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4270 a         4979 c         4407 g         4255 t
ORIGIN      
        1 gctcaacaga tcacggcaat cgttcgtttt ttatactact cagggataaa aaaaggcccc
       61 tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat gatgcaacgg
      121 taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa ccgccgcccc
      181 gtctacgaca taacgcgaat actcgtccgc gtgtgcatct gatgacatgg acaatccttg
      241 attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga cttccagcac
      301 tccggcatcg aggaagtggt gcaggttttc cgcacggact cctgctccgg ccataatgat
      361 tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca gtgcgtctga
      421 tttttgccct gatgtcagta cccgggcaat gcccaattcc gtaagattat tgagtgtatt
      481 taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc
      541 agccattatt ttttccattc gtggcatatc gacattcccg tcaacatcga gaacgcccgt
      601 caccagtcca ggaaaaccta attcgcggac tgtgcgcaca tcttcaagaa tggcggcaaa
      661 ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcgg ataatcggat gcactgggat
      721 cgtcacccgc tggcgcacgg atttcagtac ccccaacgac ggcgttaggc ccccctcttt
      781 tggagctgcg cataattcaa ctctgtcagc gccgttttgc tgcgccgtta gcgcgcattc
      841 catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttagttac gccgactgct
      901 cgctggcaac gatctcttca atggaccacg gatgaaactt aatggttgtt ttaccatcgg
      961 tgaccgccag cgtcggattc ggtaatcgtt catgctcacc ttttggcgaa ctggttttaa
     1021 ctgagatccc cggcaggctg agtccttcat cagaaagcaa tgccagcgca cgggcattca
     1081 gcgtttctgg atcacccggt agaacgattt caatatgttc ccaaccttcg tgcgggtaac
     1141 gtttttcccc gggccacggt agctccacaa tggaaaactg ccagtgcgca acctgtagcg
     1201 gttcatgcaa tttaaacaaa caaatcggtc tgccattgat catattttct gacaaaagct
     1261 cgccgcactg ttcaaacccg cgacgccagc gttcagcggt gacgttttga tggcaacgca
     1321 aagaaatgtg atcggcagtc agcggagtga tatccaaccc cagacggcgg gaaagttcat
     1381 ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt
     1441 gccagttcgc cataatcatt cttcgtcttt cattaaaagc gttaatttac cctgttgccc
     1501 tgcgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg
     1561 ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccgccc taatttcttt
     1621 aactggtgcg ggtaattttt gctcgcttca tcaatgtaag gtattccggt gaatattcag
     1681 gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc
     1741 gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc taacggcatg
     1801 atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca ggtgctgact
     1861 catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg ctttatcaac
     1921 attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc gtccgatcgt
     1981 ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc gccaaacgtg
     2041 gcgaaagaga tgcatgtcgg tcacctgcgc tctaccatta ttggtgacgc agcagttcgt
     2101 actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga ctggggcact
     2161 cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc cggtgaaatg
     2221 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaggatgaa
     2281 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     2341 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     2401 cgtctgaacg tgacgttgac ccgcgatgac gtcatgggtg aaagtctcta caacccaatg
     2461 ctgccgggta tcgtggcgga cctgaaagcc aaaggtctgg cagtagaaag tgaaggcgcg
     2521 accgttgtat tccttgatga gttcaaaaac aaggaaggcg aaccgatggg cgttatcatt
     2581 cagaagaaag atggcggcta tctctacacc accactgata tcgcctgcgc gaaatatcgt
     2641 tatgaaacac tgcatgccga tcgcgtgctg tattacatcg attcccgtca gcatcaacac
     2701 ctgatgcagg catgggcgat cgtccgtaaa gcgggctatg taccggaatc cgtaccgctg
     2761 gaacaccaca tgttcggcat gatgctgggc aaagatggta aaccgttcaa aacccgcgct
     2821 ggcggtacgg tgaaactggc cgatctgctg gatgaagccc tggagcgtgc acgtcgtctg
     2881 gtggcagaaa agaacccgga tatgccagcc gacgagctgg aaaaactggc taacgcggtt
     2941 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc
     3001 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
     3061 acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg aagagcaact ggctgcagct
     3121 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
     3181 gaaaccctca ctgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
     3241 gatctggccg gtctgttctc tggcttctat gagcactgcc cgatcctcag cgcagaaaac
     3301 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cggcgaagac gctgaagctg
     3361 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgcgtta tacggcaaca
     3421 gccgatgtca ttgcagacat cggctttttt atcattaacg ataatttaca atcacttcat
     3481 tccgttgaat ctttagcggc ggagttagcc gcccaccgcc aggcacttcc cagataaagc
     3541 gcaacggttc tgccgccggt ataccggaaa aggccatggt ggttccactc tgcccctcca
     3601 attcgacaca acgagattgt gaacacaagc gaacccgcag tcctgcaggt gtcggaccga
     3661 taagctgata acgccacgcc accagcgtca tcaatcctga agcaggttgt cgcgtagaaa
     3721 gaggcgctga cgacatcgat tcaccgcgat gatttagcgt aacgcccacg ctactcgcct
     3781 gccacatccc ctccccggcg gcttgcacca gcagcggaaa caataatatt gctaataagg
     3841 ctctcattat ttgccgccaa tcgtcgccgt catacggata tgtcggttat ctgatagttc
     3901 cagattcgac agcaccacta actgcggcaa gctgcggcgc aggaagcgag acaataatgg
     3961 tcgtagcgcg tggttcacca acaataccgg cggcgcaccc aacatctcct gacgggatag
     4021 cgcttcctga gtttgcgcca gtaaacgatc cgccagccct ggctccagtc caccaccgcc
     4081 ttgcaacgcc tgtagcaaca aacgttccag cggtgtatcg aggccaataa catggacttc
     4141 atctttgcca ggaaaccact gctgggtaat cgcccgtccc aacgctacgc gcacgacagc
     4201 ggttaattca tgcggatcgc tttggatggg cgcatgttcc gccagcgttt cgagaatggt
     4261 gcgcatatcg cgaatcggca ctttttcatc gagcagattt tgcagcactt tatgcagtgt
     4321 ggtaagcgtg acgacgccag gaacgagatc ttccgtcagc tttggcatct cctgggcgac
     4381 gcgatccagc agctgttgtg cctcctgacg accaaacagc tctgcggcat gctggctaat
     4441 gaggtggtta agatgcgttg ccaccaccgt gctggcctca accacggtgt acccctgaat
     4501 ctgcgcctgt tctttaagcg cactttcaat ccagatagca ttcaggccaa atgccggatc
     4561 aacggtcgct tcaccaggta atgttccggc ggcagtgcct ggattaattg ccagccagcg
     4621 ccccggataa gcatcaccac tccccatttc gacacctttc atcaagatac gatagcgggc
     4681 tggttgcaaa tccatgttat cgcggatgtg caccactggg ggtaggaagc ccatctcctg
     4741 ggcaaatttc ttgcggatac tgcgtatacg gcccaacaac tcaccatcct gctgaaaatc
     4801 aaccatcggg atcaaccgat accccacttc cattcccaga gaatcttcca gttgtacatc
     4861 gttccacgtt gcttcgacta ccgaattatt ctctgccatt tttaccggtt ttggttcggc
     4921 aggcgctttt tgttcacgtc cgcggatcca ccaggccagc cccagcaatc cggcagtgaa
     4981 cagcaaaaat accaggtttg gcattccagg caccaggccg agtaaaccga gcacggcggc
     5041 gcttaataac ataacgcttg ggttactgaa aagctgattc accatctgct cgccaacatc
     5101 ctgatcggtg ctgacacgcg taacgatgac ccccgcggcg gtagaaatca ccaacgccgg
     5161 aatttgtgcc accagaccgt cgccaatggt caatagcgta taactttccg ccgcgtgccc
     5221 catgctcatg ccatgttgca gcacaccgac cagcaatccg ccgacaacgt taatgaccat
     5281 gatgaggatc ccggcgatgg catcgccgcg aacaaactta cttgccccgt ccatcgagcc
     5341 gtaaaaatcg gcttcctgag tcacttcgga gcggcgtttt ttcgcttcat cttcaccaat
     5401 caatccggcg ttaaggtcgg cgtcaatcgc catctgctta cccggcatac catcgagaac
     5461 aaagcgcgcg cccacttccg caatacgccc ggcacctttg gtgataacca taaagttgat
     5521 gatcacgaga atgacgaaca ccacgatacc gatagcgaaa ttgccaccga cgaggaagtg
     5581 accgaacgct tcgaccacct ttccagccgc cgccgcgccg gtatgccctt ccatcaaaat
     5641 gatacgggtt gaagccacgt taagtgccag acgcaacagc gtggtaaaca acagaatggt
     5701 cggaaacgca gcaaactcga gcgtgcgctg ggtaaacatc gccaccagca acaccatgat
     5761 cgacaaggca atattgaagg taaacagcag gtcgagtatg aatgcgggca gtggcagcac
     5821 catcatcgac aagatcaaca ggatcaaaat cggtccggca aggatctgcc attgtgtcga
     5881 tttcaggttt gcgggcaggc gcagcatcgc ggccagatta ctcatgggtc ggtttctcgt
     5941 taataaaatc cagggcttcc ggcaccggaa gatgagtagg ttgtacaggg cgctgtccac
     6001 cagccagacg ccagcgtttc agttgccaga cccaggccag cacttccgcc accgcggcgt
     6061 acagttgacc cgggatttgt tgaccgatct ccgcatgtcg atacagcgca cgcgccagcg
     6121 gcggcgcttc aagcgtcggg acgttatttt cagcgccaat ttcacgaatg cgcagcgcga
     6181 ccagccctgc acctttagcg accactttcg gtgcgctcat tttgttttcg tcatactgca
     6241 acgctaccga atagtgggtc gggttattga caatgacatc cgctttcggc acatcggcca
     6301 tcatccgacg ccgtgcggcc gctcgctgca tctgacggat ccgcccttta acatgggggt
     6361 cgccttcgct ttgtttgaac tcatcacgaa tatcctgccg tgacattcgc agctttttca
     6421 ggtggctgaa gatttggaaa aagacgtcaa atcccaccat tggaatgaca ccaagcacca
     6481 ccagcagcgc gcatagccct accagatcca ttgcattacc catggcggta atcggagact
     6541 cggccattaa gcgcatcatc tgcggccagt gataccagag aaaaaacccc gtcacgctgc
     6601 caaccaggat ggttttcaaa attgccttaa gcaactccgc gccagtctgt gccgagaacg
     6661 tccgtttaat gcccggtagc gggttgagtt tggaaaactt cggctgcaag gattttccgc
     6721 taaataccag cccccccagc ataaccggag aaataatcgc caccagcacc acgccgctaa
     6781 tcagcggtag cagcgccagc atggcttctc tgatcagcag aataatctgc ccgaggatca
     6841 gattcgggtc attgataata ctgtgatcaa aatgtagccc tgcggagagc atgcccgaca
     6901 atcgacgggc cagcgacaca ccgccaaacc agataacact aacgcccacc agcaaaatca
     6961 gcagtgaagt cagttcacgg gaacgcggga tttgcccctc ttcccgcgct ttttctagtc
     7021 ggtgaggtgt gggggcctct gttttgtcgt cgctctcgtc agacacgtcg ccaatcctgg
     7081 aaagagtatt aagcgtaaat gatgccagag cgcaaagcgt tcaatggttt gagtaagggg
     7141 caaaacaggc gggatttagg gcttttactg ccacacatca agcatagtat gcgtttgtcg
     7201 gatgcggcgt catcgcctga tatgacgtgg tcacgtcaca tcaggcaata caaatcaaaa
     7261 tccaagacta tccaacaaat cgtccacctg accctgactg gctaccacac cggctttgct
     7321 ggtatcgacc tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg
     7381 ttccgggatg ttttccaaca acaccatcag caactgacgt tcgatctcct gaatgacatc
     7441 catcatccgc ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc
     7501 cagcagttgc gcgttagtaa agctggtatg cgcggggaca tctgccagaa attgccgtgt
     7561 atcgatcacc agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca
     7621 acgttgggtt aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc
     7681 aacactgttc agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg
     7741 atcgcgcgca tcggggatgg cttccgctgc ttcgacaatg gcctgatcca gccccagttc
     7801 ccgcaaactg tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc
     7861 tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca
     7921 gtttctcaaa gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca
     7981 catagccact ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag
     8041 tcaccattaa cactggcaat gccgacatcg cgccatccgc acgaatcgtt ttcagcaact
     8101 ccaggccatc catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct
     8161 gcaacttatt gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct
     8221 ctttcagcag gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt
     8281 taagttcttt atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact
     8341 aatttttgcc agcatttgct gacttacctg gctaagatcg accacttcgc agacaccacc
     8401 catattgatg gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag
     8461 ggtccatgcc cccgcctgac gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc
     8521 ggttaggatt accccaaccg cattacgccc cgcctgtttg gccacagaat ggaacaacac
     8581 atctaccgat ggccgatggc gattaaccgc cgggccatcg tgaattttga tttgataatt
     8641 tgcgccacta cgcgccagct ccatatgccg atcgcccggc gcgatatagg catgtcccgg
     8701 caagacgcgc tctccatctt cggcttcttt aaccccgatc tggcaaagct tattaagtct
     8761 gtcggcaaaa gagcgggtga aaccgggcgg catatgctgg gtaattaaca gtgccggact
     8821 ggaaagcggc aacggttgca gtacgtgacg aattgcctca gttccccccg ttgaagcacc
     8881 aatcgcaatc agtttttcag aactcaacaa cggccccgcc ttcagcgttg tcggtgccga
     8941 caatggctta tgtgctgcaa ggctcgcctt tgctgccgta cgcacctttt cagcaatcat
     9001 ttcgctatac gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat
     9061 cgcccccaac tccagcgcgc gcagcgtgac ttctgaccct ttgccggtca gggaagaaac
     9121 catcacaacg ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat
     9181 ccgcggcatt tcaacatcca gcgtcaatac atcgggattg aatttcttaa tcaaatcacg
     9241 cgcaaccagc ggatcagggg cggtcgccac catttccatg tcgctatggc tgttgatgat
     9301 ttctgtcatg atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct
     9361 catcgttaat ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca
     9421 aggtggctaa agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgaa
     9481 acaaagcggc gcaaaatctc ctgctgggta gtttgatcga agtagatcat gacgttacga
     9541 cagaagatcg catcaaacgg ccccggcacg gtgtactgtt tcgccagcaa attcagcggg
     9601 gcaaaatcaa catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc
     9661 gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg
     9721 cgataaatac cgcttctggc tttttccagc acttcggtgt cgatatcgct ggcaaacact
     9781 ttccagcgtc cgggcgcggt gcccaatgta tcagccagcg tcatcgctat gctgtacggc
     9841 tcttcgccgg ttgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg
     9901 tgatccgcga gcagaggaaa atggtgtgcc tcacggaaaa atgccgtcag attcgtggtc
     9961 agcgaattga taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcagg
    10021 taatgaccga aatccgccag tcccagcgca cgcaaacgac gaaccagtcg gttgtaaacc
    10081 atgtcgcgtt tatggtcagc cagaacgatc ccggcgcgtt gatagatcaa ttgacttatc
    10141 cgccgaaaat gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt
    10201 tgcccacagg gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc
    10261 gcaatttgta actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc
    10321 gccacacgcg acgaaagatg gtcggcctga ttcagtcgtt gcaaatcgtc gcgaaatgcc
    10381 caaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    10441 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    10501 ctttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    10561 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    10621 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    10681 ccagcgcagc tgcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    10741 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acgactctgt tcatccgatg
    10801 ctgatgcaat ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca
    10861 ttgtttcccc tgcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
    10921 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    10981 cgcccgccac cacggcaaaa ccacggcctt gttcacccgc tcgcgcggct tcaacagcgg
    11041 cattcagcgc gaggatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
    11101 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    11161 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    11221 tatcggcgtt ttgcttcact gtcgcggcga gctgctccat gctggcggca gtttcttcca
    11281 gcgcggatgc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    11341 gggtaccggc atagatggca tctgaacctt cgcgcacatg agtgacggtg tcagtcaaag
    11401 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    11461 tcagggtatt cgccaggtta ccaccggcaa tttcgcgaat atgcgcaata atttttgcca
    11521 gcggtgtaag caacatacgg cgaatgccgt accacgccgc cagcagaatc aataccacca
    11581 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    11641 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tctcccattg
    11701 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    11761 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    11821 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgcgg
    11881 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcatta ctttgctgat
    11941 tggatgaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgtagca
    12001 ttaaatccca tgttgacgtc agctcgctct gctgttcccg taattgattg gaaaccacaa
    12061 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    12121 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    12181 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaggggggaa actttattgc
    12241 tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cgggttcatt
    12301 tcaattgagg acatcgggag aattacgcca cttctgacgc cgcgctatct aacagcgcca
    12361 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatgcgg tcgcccagtg
    12421 cgcccagtcc tgtgagatat tcggttgaaa gcgtcacggc aaattccggt gccggacgaa
    12481 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    12541 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    12601 tgaacttaat tcgtaagtca acaatcggca caataacgcc gcgcagattc gtgacgcctt
    12661 tgataaacgc tggcgtgttc gcaatccgtg tcacctgatc gtagccacgg atctcctgca
    12721 ctttcagaat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    12781 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    12841 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    12901 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    12961 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac caattaattg
    13021 atccactagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    13081 ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga taggcagata
    13141 ttcaccccgc acttccagca cccgctcgcc gccagccagt ggatgcagat cggcttcacg
    13201 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    13261 tacggacatg ccgtcaagaa ttgccagcgt cagcggcagt aaaatgcgga tcgtagtgcc
    13321 agtaccctgc atcgactgga tctcgacatg accgcccatc tcctggatat tacgtttaac
    13381 gacgtccatg ccgacgccac gcccggagac gtcggtgacc tgctccgccg tggagaagcc
    13441 aggtgcaaat atcagcattg caacttcgtc gtcgctcatg ttttcgctga cggtcagacc
    13501 ttgcgaggcc gcttttgcca gaattcgttc acggtttagc cccgccccat cgtcggtcac
    13561 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    13621 tttacctgca gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    13681 caggtgggtc agcgggtcga taatgcgttc aatcaggctc ttgtcgagtt cagtagaact
    13741 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg
    13801 gggatagcga ctaaagacat attccatcgg catcatgcga atcgacatca ctgattcctg
    13861 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    13921 cgggtccagt tcgctggaac gctgggcaag catggactgg gtgatgacca gctcaccgac
    13981 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    14041 gcgcgtcgtt ttttcccgct ccacgcgacc ggttggcgct tgttcggctg ccagtttaag
    14101 cattggtgag gtggatattt ttggcgagac gtctactgtt tcaaaggtaa tctgatcggc
    14161 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    14221 tgccgagagg gaatcagccc ctttcaccac gtcagttaac gttgtcagat gccccagctc
    14281 ttcttccagc aggtcgactt cgccggcctt caggcgcgaa aggataattc gtcgcggtga
    14341 ctgactacga ctctgctcat cttgtggttc acttttggca accacactta atcgggtcac
    14401 tgcggatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    14461 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    14521 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    14581 tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    14641 aaaggttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    14701 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatatcag ccaacagttc
    14761 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    14821 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    14881 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    14941 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    15001 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcca ttcaacccgc
    15061 caaccatcag ttcgcggcgg gatgcattgg cacgatcggc agaaagctcc cagttgctgt
    15121 aacctttctc accgctggcg tagggaaaat catcggtatg acccgagagg ctaattcggt
    15181 tgggaatacc gttcagtacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    15241 cgtcggcact gccagttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    15301 gaccttcctg gaccagatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    15361 actcaatcaa ctggtcgaga tcgccacgca atttccgcaa tcgactttgc tccatgcgtt
    15421 ttttcagctc ttcgatgttc ggccgcttat tcacttcccc ctggctttgg gtgtaatcat
    15481 caccaccgcc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgctg
    15541 tcgccagcgg agtccggaag tactccgcaa tctgaatcag ctcttttgga ctggagatag
    15601 agatcagcca catcaccaga aaaaaggcca tcattgcggt cataaagtcg gcataagcaa
    15661 tcttccacga tccatgtgct gccccgtggc ttttggcttt acgtcgtttg acgacaataa
    15721 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    15781 cgcacgcaca tgctcttcca gctcaataaa cgacggacgt tcgctggaat agagcgtttt
    15841 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agatttgaaa gcagagtgac
    15901 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    15961 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgagc
    16021 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaactacccc
    16081 cattacagcc gcaacaatac caaaagccgg aagtgagtcc ccgaccagcg ccagactgtt
    16141 cgccgggact tctgcctcgc tttcgtgcgt ctcaatttct tcatccatca gggcttcgat
    16201 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaattcaag
    16261 catgacgcta tccgcaagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    16321 ttcaatatcg cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    16381 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    16441 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    16501 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    16561 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    16621 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    16681 cacgcgatag gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgtttcgcc
    16741 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    16801 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggct gacataagct
    16861 gcaggcaaag ctgccaacag gctggtgagc atgggtaata aaattgccgc cgcagcagtt
    16921 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    16981 taatgccagc agcggtcctt cttctgcttg cgggcactgt tcaaggtata aacggtaggc
    17041 tttgatcacc gcatcgacgc cattacacaa gccagttttc agtaaaaact gccatgcatt
    17101 acagaacatc gaagcatgaa cattttgttc ccaggtcata aaccagtcgg ttgaaaatgg
    17161 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta tagagtttta tcagacgtcc
    17221 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    17281 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    17341 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    17401 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    17461 atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    17521 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    17581 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    17641 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    17701 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    17761 aaaaaataat tagcattaga atagttgcga taagctgcaa taagcagacc cacctttttg
    17821 gtttaataag tccttacaaa tagaaggggg tctttacact tatctaagat ttttcctaaa
    17881 tcgacgcaac tgtactcgtc actacacgca c
//
LOCUS       DAJOTB010000097        17813 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJOTB010000097 DAJOTB010000000
VERSION     DAJOTB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.097"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(319..909)
                     /locus_tag="OHN28_003224"
     CDS             complement(319..909)
                     /locus_tag="OHN28_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298563.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(959..2155)
                     /gene="hemY"
                     /locus_tag="OHN28_003225"
     CDS             complement(959..2155)
                     /gene="hemY"
                     /locus_tag="OHN28_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCQ2298564.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2158..3363)
                     /gene="hemX"
                     /locus_tag="OHN28_003226"
     CDS             complement(2158..3363)
                     /gene="hemX"
                     /locus_tag="OHN28_003226"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2298565.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     APAAAPQGE"
     gene            complement(3385..4125)
                     /gene="hemD"
                     /locus_tag="OHN28_003227"
     CDS             complement(3385..4125)
                     /gene="hemD"
                     /locus_tag="OHN28_003227"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCQ2298566.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4122..5063)
                     /gene="hemC"
                     /locus_tag="OHN28_003228"
     CDS             complement(4122..5063)
                     /gene="hemC"
                     /locus_tag="OHN28_003228"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCQ2298567.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5450..7996
                     /gene="cyaA"
                     /locus_tag="OHN28_003229"
     CDS             5450..7996
                     /gene="cyaA"
                     /locus_tag="OHN28_003229"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCQ2298568.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(8036..8356)
                     /gene="cyaY"
                     /locus_tag="OHN28_003230"
     CDS             complement(8036..8356)
                     /gene="cyaY"
                     /locus_tag="OHN28_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCQ2298569.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8482..8667)
                     /locus_tag="OHN28_003231"
                     /pseudo
     CDS             complement(8482..8667)
                     /locus_tag="OHN28_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8826..9029
                     /locus_tag="OHN28_003232"
     CDS             8826..9029
                     /locus_tag="OHN28_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2298570.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9066..9890
                     /gene="dapF"
                     /locus_tag="OHN28_003233"
     CDS             9066..9890
                     /gene="dapF"
                     /locus_tag="OHN28_003233"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCQ2298571.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGIGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9887..10594
                     /locus_tag="OHN28_003234"
     CDS             9887..10594
                     /locus_tag="OHN28_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCQ2298572.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10591..11487
                     /gene="xerC"
                     /locus_tag="OHN28_003235"
     CDS             10591..11487
                     /gene="xerC"
                     /locus_tag="OHN28_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCQ2298573.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11487..12203
                     /gene="yigB"
                     /locus_tag="OHN28_003236"
     CDS             11487..12203
                     /gene="yigB"
                     /locus_tag="OHN28_003236"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCQ2298574.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12287..14449
                     /gene="uvrD"
                     /locus_tag="OHN28_003237"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12287..14449
                     /gene="uvrD"
                     /locus_tag="OHN28_003237"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCQ2298575.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14504..15406)
                     /locus_tag="OHN28_003238"
     CDS             complement(14504..15406)
                     /locus_tag="OHN28_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCQ2298576.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15489..16253)
                     /locus_tag="OHN28_003239"
     CDS             complement(15489..16253)
                     /locus_tag="OHN28_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCQ2298577.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16623..17573
                     /gene="corA"
                     /locus_tag="OHN28_003240"
     CDS             16623..17573
                     /gene="corA"
                     /locus_tag="OHN28_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCQ2298578.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4115 a         4664 c         4727 g         4307 t
ORIGIN      
        1 gagaaatatt cactcaggaa gttattactc aggaagcaaa gaggattaca gaattatctc
       61 ataacaagtg ttaagggatg ttatttcccg gttctctgtg gcataataaa cgagtagatg
      121 ctcattccat ctcttatgtt cgccttagtg cctcataaac tccggaatga cgcagagccg
      181 tttacggtgc ttatcgtcca ctgacagatg tcgcttctgc ctcatcagac accatggaca
      241 caacgttgag tgaagcaccc acttgttgtc atacagacct gttttaacgc ctgctccgta
      301 ataagagcag gcgttttttt atgaccgagt aaacaacagg ctacgtcgct cttgggtcat
      361 cggttgtgcg tttaatacac ttacaagaga acctaccgtg gtgatgggaa gaaacggatt
      421 atcttctatt ccgcgtagat cgcgcccaca acgagcggca atatcttcga ccagctcttc
      481 taactcgtct gcatcgttta ctaaatccag atcatcgtcc agtttatcac tggctttcag
      541 tggtaatgga taatcgatat agccgtttcc ttgtaaggta ttccacacag cccgaatcac
      601 ccatgtatct acggtgtggg gggaaaattg gcgtgcgaac tgacaaatgg aaagtccctg
      661 acgtttatca gccataatct ggcgcttctc gatgagcttg attttttcct gcttcgcaat
      721 ggctatcaga tacccaataa aaagtgttaa ccagaagatg aatgtattgc ctgtacgtac
      781 gaaggacaga atcagccaac ttacgccagc cagaacggca catatcaaca aaatcagcaa
      841 gagcgttgcc cgaaaaggat taatttcatg tgatggtaag cggaaaatat agcgggacgg
      901 tgtgcgcatg tttgacatcc ttgtaaaaaa atatcgcccc tgaacgaggc gatatagatt
      961 actgcggtgg gttgttttgc aacgttagca tcaaaccgtc gcgacgcatc gctgcggctt
     1021 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
     1081 gacgttgttt cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgtttca
     1141 tcagcgactg acccagtgtg ctccacaaca gcgggcgatc gcccacgttt ttgatctgtt
     1201 ggcgcagcac tttttccagc tgttccggat tattggtttt cagacgcggg atcggcagca
     1261 gcaggcgatc gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat
     1321 gatcgtcaca ctcaataaga tgttccgcca ttgccacctg caaggccacc tgatgacgcg
     1381 ttttccggct ttggtttttc caccagttac gcaaaccttc gctaccgtta tcggcacgcg
     1441 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
     1501 caacatgggc tttcgccatt gatgggatga tatccagcag cgaactccat gcccctgtgc
     1561 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1621 gcttatcaac gccgtgacgt gctgcatggt tttcattacg ggccagttgc agacgtacac
     1681 gggtgatttc taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt
     1741 ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggctgccagt agatagttca
     1801 ccaccggttg ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat
     1861 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgtgcacgg cgacgcttac
     1921 gtccgacaaa ccacccacgg gtgtgcgcgc ctgtgcggaa gattcgccgc agtagccact
     1981 caatggcaaa cagcactacc atcgccagaa tcaaaataat cgcgaggccc gtgacgctag
     2041 tttcaatatt gtagttgtcg gtctggatca gcacgtaacc ctgatggccg gcaatcatcg
     2101 ggccaaccac gatccccgca atcagcaaca caaagagcaa taacactttt agcatggtta
     2161 ttctccttgc ggcgcggctg ccggagcatc agcttgcggt gcaggtgcag gtgcaggtgc
     2221 aggtgcaggt gcaggtttag cttccgttgt ccctgctgcc ggttgtgcca gcaggttacg
     2281 cacgcgggtc tgcatcaatt tttccagcat cgcctggctt tgcagggttt ccggaagatc
     2341 catcgagata ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc
     2401 atcatcagta tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata
     2461 agtctcttcc tggtgacgcg gtacagcttg tgctgcgacc agcaggcgag agcggatatt
     2521 ttcgcgcaga tagatatcct gatttggcgc taacagcggt acggcagtgt catcacgacg
     2581 gcgaatcgta atgaagttgt ccataaaatt ctgccagctt ttttgcagat tgatacgcca
     2641 ttcgctgatg gaactggaaa gctcttcacc gtcggaatcc atcggcgaac catcgctgtc
     2701 attatcggcc agacgcagat tatctacctg atttgaaagc tgattaagct taaggatgat
     2761 accgtcataa tccacctgcg ataccgcaga aaggctggcg atatcatcgg taattgcccg
     2821 gcgaacggta atcagactcg ggtcattcat atccgccagg ctggcgtctg cacttttcag
     2881 caacgctgca gcggtcgtga cgtcctgatc gctccacagc ttccgtccgg cgagtttcac
     2941 cagaaaatcg gcctgggcca gcagccaggt tttagcatcg ctgccggaaa tggtggcgac
     3001 cttttgttgg acttcatcca gctgttttgc cagcgtttct tgctgacgat tcgcctgctc
     3061 aagctgtaca gcttgttgct tgataatgcc ttccagctcg gctttttggc tctcctgggc
     3121 tttttgcagt gccgtaagtt ggttagccag ggcatcgctg gtggcagtct gattgacggc
     3181 ctgttgttta ccccagccat acaaaccgac gcccgccgcc agagcaatag cgatagccac
     3241 cgcgctgaga atcaatgcgg tattgttctt actctttttt tctgttgcga caggttgtga
     3301 cgtggtgtcc acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt
     3361 cattatggct tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc
     3421 gttatcggcg accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc
     3481 actgacgacc aatagtcgac agcgtagtaa ccagtgctca cgataccatt gtgggatcag
     3541 cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg accgtcgtca cctcgcggga
     3601 ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt gataacattc
     3661 acaaaaagtg acctcagcac cgcgcgccgt cagggaatcc ccaattagct cacgaccgcc
     3721 attgccacgt aatatcagcg cacatttgcc cgcaatattt tgtaattcag gtaattgtag
     3781 caagacttcg ctgatttccc gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg
     3841 tagtgccagc gcggtggtgc gtccaatggc gaaataagta ggtagtcggg gccatttacg
     3901 atcttgctga tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa
     3961 cagatcgctc tctcccagcg ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg
     4021 agaaaactca atcagcggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac
     4081 taactcttct cctgcgggag acgggcgggt gaccaggata ctcatgccgg ggcgtctccg
     4141 ttatagactt cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa
     4201 atccccattt gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatcagcgaa
     4261 ccgtccggcg cgccgaccaa cgcacgcagc cagatttcgc catcaataag ctcggcgtag
     4321 ctaccaattg gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttcggcggta
     4381 acacgcagtg cagtttcgtg gtgattcagc gcggcgagca gctcgcgagt gcgtgcatca
     4441 tcaaggcggc attcaatgcc caccgcacct tgccctaccg ccggaagaga aatctcgggt
     4501 ggcaacgcgg cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc tacggcaaga
     4561 atgatggcat cgtattcacc gttatccagt ttgctcaggc gagtgccgac gttgccgcgc
     4621 agggagcgga taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg
     4681 gacgtcccga cgatactgcc tgccggtaac gcatccagac tgtcatagtt attggacaca
     4741 aatgcatcgc gaggatcttc acgctcgcaa atagtgacca gccccagacc ttgcgggaat
     4801 tcaaccggca catctttcat cgaatgtacg gcgatatcgg cgcgattttc gaggagcgcg
     4861 acttccagct ctttaacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga
     4921 atcacatcgc cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc
     4981 atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca
     5041 attcttaaaa cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa
     5101 catccttgcc agagcgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa
     5161 aatgaggtcg ggcagtggat cctgacaggc gctttgagcc ttcgtaataa ggaatttaca
     5221 gagaataaac ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg
     5281 tgttaaattg atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg
     5341 aaaagtggta acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg
     5401 atgttggcgg aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta
     5461 tattgagact ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct
     5521 tgctgctatg gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta
     5581 tcaccatccg ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta
     5641 cacgcctgat gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc
     5701 agtacaggac ccaccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac
     5761 ctcgtccgta gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg
     5821 gctcgatagc gaagagcgcc agttgctaca acgtaaatgt agtctgctgg aaagctgggc
     5881 cgcctcgctg ggtgtggaag tcagcttctt cctgattgat gaaaaccgct tccgtcataa
     5941 tgaaagcggc agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga
     6001 cgaattttat cgtaccgccg tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc
     6061 gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct
     6121 gacgccaaat gaatggctgg atcttggtgg cttaagctcg ctttctgctg aagagtactt
     6181 tggtgccagc ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa
     6241 aacactgctg ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga
     6301 tatcaaacag cgtttgcacg acggcgagat tgtatcgttt ggcctcgacc cgtactgcat
     6361 gatgctggag cgtgttactg aatacctgac ggcgattgaa gatttcaccc gtctggattt
     6421 agtgcgtcgc tgcttctatt taaaagtgtg cgaaaagctc agccgtgaac gcgcctgtgt
     6481 aggctggcgt cgcgcagtgt tgagccagtt agtgagcgag tggggttggg acgaagctcg
     6541 tctggcaatg ctcgataacc gcgctaactg gaagatagat caggtgcgtg aggcgcacaa
     6601 cgagctgctc gacgcaatga tgcagagcta ccgtaatctg atccgctttg cgcgccgcaa
     6661 taaccttagc gtctccgcca gcccgcagga tatcggcgtg ctgacacgta aactgtacgc
     6721 cgcgtttgaa gcattaccgg gtaaagtgac gctggtaaac ccgcagattt cgcccgatct
     6781 ctcggaaccg aatctgacct ttatttatgt accgccgggc cgcgccaacc gttcaggttg
     6841 gtatctgtat aaccgcgcgc caaatattga gtcaatcatc agccatcagc cgctggaata
     6901 taatcgctac ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctctcg
     6961 cacccgtttg tatattaaag gtaacggcat tgtcgatttg cctaagttgc aggagatggt
     7021 cgcagacgtg tcgcaccatt tcccgctgcg cttacctgca ccgacaccga aggcgctcta
     7081 cagcccgtgt gagatccgcc atctggcgat tatcgttaac ctggaatatg acccgacggc
     7141 agccttccgc aatcaggtgg tgcattttga tttccgtaag ctggatgtct tcagctttgg
     7201 cgagaatcaa aattgcctgg tgggtagcgt tgacctgctg tatcgcaact cgtggaacga
     7261 agtgcgtacg ctgcacttca acggcgagca atcgatgatc gaagccctga aaactattct
     7321 cggcaaaatg catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gttatagcca
     7381 gcatctgcgc gggttaatcc gtactcgcgt gcagcaactg gtttctgagt gtattgaact
     7441 gcgtctttcc agcacccgcc aggaaaccgg gcgcttcaag gcgctgcgcg tctccggtca
     7501 aacctggggc ttgttcttcg aacggctgaa tgtatcggta cagaaactgg aaaacgccat
     7561 cgagttttat ggcgctattt cgcataacaa actgcacggc ctgtcagtgc aggttgaaac
     7621 caatcacgtc aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt
     7681 cttcttcgaa gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa
     7741 ccgggttgag gtgtatcacc actgcgaagg cagcaaagag gaactggtgc gtgacgtcag
     7801 tcgcttctac tcgtcttcgc acgaccgttt cacctacggc tcaagcttca tcaacttcaa
     7861 cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgt gaacaggtga ttccgtttcg
     7921 caccaaatct atcggtaaca tgccacctgc caatcaggag catgacgcgc cgctgttgca
     7981 gcagtatttt tcttgatgct aatgccggaa agcgaggctt atccggcatt agctcttaac
     8041 gaaaactgac ggtttctccc gcctgctgcg tcgccgcctg ttccagcaaa tcccagaagg
     8101 tttcgccgct gcgatcgcaa atccactcat cgcctttcag atcaaaatgg tagccgccct
     8161 gtttggttgc cagccatacc tggtgcagtg gttcctggcg gttgataatg attttgctgc
     8221 cgttctcaaa ggtaatggtt agtacaccgc cgttgatttc acagtcgata tcgctgtcgc
     8281 catcccagtc gtccagacgt tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa
     8341 attcactgtc gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg
     8401 gggtcagggc ttcaaagttt gcccgtctgc tgctgcgttc cggcatgatt catccgtcac
     8461 cggaataata atatctctat atagcgaaag attggtcttt tcataagggc gcaaatacac
     8521 cacgcctttt ccctgtttct ggtaaatatt atgttccggc tcatcaagcc gacatcccca
     8581 tgccgatgat aagaacaacg aacaagacaa taaacaaacc acattgcgat agtgcataaa
     8641 gccatcctgg cgcgaggtgc cgatcacgaa actaccagca aaacataaat cgccacgagt
     8701 aagcgttata ctcgcagcat ttcctcactt ttcagacttc ataaagagtc gctaaacgct
     8761 tgcttttacg ccttctcctg cgatgataga aagcagaaag cgatgaactt tacaggcaat
     8821 ccataatgaa aaacgtgttt aaggcactca ctgtattact tactctcttc agcctgacgg
     8881 gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga taaaaacgca ccgccgccga
     8941 ccaaaccggt agagacgcaa acgcaatcca cggtgccgga caaaaacgac cgcgccactg
     9001 gcgatggtcc atcccaggtg aattactaaa agtcagtttc tgtacccgcg tgattggagt
     9061 aaatgatgca gttctcgaaa atgcatggcc ttggcaacga ttttatggtc gtcgacgcgg
     9121 taacgcagaa tgtctttttt tcaccggagc tgattcgtcg cctggcggat cggcacctgg
     9181 ggatagggtt tgaccagctg ctggtggttg agccaccgta tgatccggaa ctggattttc
     9241 actatcgcat tttcaatgct gatggcagtg aagtagcgca gtgcggcaac ggtgcgcgct
     9301 gctttgcccg ttttgtgcgc ctgaaagggc tgaccaataa gcgcgatatc cgcgtcagca
     9361 ccgccaacgg acggatggtg ttgaccgtca ccgatgatga tctggtccgc gtcaatatgg
     9421 gcgaacccaa cttcgaacct tctgccgtgc catttcgcgc taacaaagcg gaaaagacct
     9481 atattatgcg cgccgccgag cagacaatct tatgcggcgt ggtgtcgatg ggaaatccgc
     9541 actgcgtgat tcaggttgat gatgttgata ctgcggcggt agaaacgctt ggtcctgttc
     9601 tggaaagcca cgaacgtttt ccggagcgcg ccaatatcgg ttttatgcaa gtggttaagc
     9661 gcgagcatat tcgtttacgc gtttatgagc gtggggcagg agaaacccag gcctgcggca
     9721 gcggcgcgtg tgcggcggtg gcggtaggga ttcagcaagg tttgctggcc gaagaagtac
     9781 gcgtggaact ccctggcggt cgtcttgata tcgcctggaa aggtccgggt cacccgttat
     9841 atatgactgg cccggcggta catgtctacg acggatttat tcatctatga agcaaccagg
     9901 ggaagaactg caggaaacac tcacggagct tgatgaccgg gcggttgtcg attatctgat
     9961 taaaaatcct gagtttttta tccgcaatgc gcgcgcagta gaagcgatac gtgtgccgca
    10021 tccggtacgc ggcaccgttt cgttggtcga gtggcacatg gcccgcgcac gtaatcatat
    10081 tcatgttctg gaagagaaca tggcgttgtt gatggaacag gctatcgcca acgaaggcct
    10141 gttttatcgt ctactctacc tgcagcgcag tctcaccgcc gccagcagtc tcgatgatat
    10201 gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg gcaggtgcga gtctgcgcct
    10261 gtttccagat cgctggcgct taggtgcgcc gtcgaaccac actcatctgg cattaagccg
    10321 tcagtctttc gaaccgctgc gtattcagcg tctggggcag gaacagcact atctgggacc
    10381 gcttaacgga ccggagctgc tggtggtgct accggaagcg aaagcggtgg gatcggtggc
    10441 gatgtcgatg ctgggaagcg atgctgattt gggggttgtg ctgtttacca gtcgcgatgc
    10501 cagtcactat caacaagggc agggaacgca gttacttcat gaaattgcgc tgatgttgcc
    10561 ggagcttctg gcgcgttgga ttgaacgcgt atgaccgatt tacacaccga tgtagaacgc
    10621 tacctacgtt atctgagcgt ggagcgccag cttagcccga taaccctgct taactaccag
    10681 cgacagcttg aggcgatcat ccattttgcc agcgaaaacg gcctgcaaag ctggcagcaa
    10741 tgtgatgtga cgatggtgcg taattttgct gtacgcagtc gccgtaaagg gctgggagct
    10801 gcaagtctgg cgttacggct ttctgcgcta cgtagctttt ttgactggct ggtcagccag
    10861 aatgaactca aagctaaccc ggcgaaaggt gtttcggcac cgaaagcgcc gcgtcatctg
    10921 ccgaaaaaca tcgacgtcga tgatatgaat cggctgctgg atattgatat caacgatccc
    10981 ctcgccgtgc gcgatcgggc aatgctggaa gtgatgtacg gcgcgggtct gcgtctttcc
    11041 gagctggtgg ggctcgatat caaacacctc gacctggagt ctggcgaagt gtgggtgatg
    11101 ggaaaaggca gcaaagagcg ccgcctgccg attggtcgca acgccgtggc gtggattgag
    11161 cactggcttg atttgcgcga cctgtttggt agcgaagacg acgcgctttt tctgtcgaaa
    11221 ctgggcaaac gcatctccgc gcgtaatgtg cagaaacgct ttgccgaatg gggcataaaa
    11281 caagggctga ataatcacgt tcacccgcat aaattacgtc actcgttcgc tacccatatg
    11341 ctggagtcga gcggcgatct tcgtggtgtg caggagctgc tgggtcatgc caacctctcc
    11401 accacgcaaa tctatactca tcttgatttt caacaccttg cctcggtgta cgatgcggcg
    11461 catccacgcg ccaaacgggg gaaataatgc gtttttaccg gcctttgggg cgcatctcgg
    11521 cgctcacctt tgacctggat gatacccttt acgataaccg tccggtgatt ttgcgcaccg
    11581 agcgagaggc gcttaccttt gtgcaaaatt atcatccggc gctgcgcagc ttccagaatg
    11641 aagatctgca acgcctgcgc caggcggtac gggaagcgga acctgagatt tatcacgacg
    11701 tgacgcgctg gcgttttcgt tcgattgaac aagcgatgct cgacgccggg ctgagtgccg
    11761 aagaagccag tgcaggcgca catgcagcaa tgatcaactt tgccaaatgg cgcagccgga
    11821 tcgacgtccc gcagcaaacc cacgacacct taaaacagct ggcgaagaaa tggccgctgg
    11881 tggcgatcac caacggtaac gcccagccgg aactgtttgg tttgggggat tattttgagt
    11941 ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt cagcgatatg tacttcctgg
    12001 cggcggaaaa actcaacgtg ccgatcggcg agatcttaca tgtcggggac gatctcacca
    12061 ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg ttggatcaga ccggaaaatg
    12121 gcgatctgat gcaaacctgg gacagccgtt tactgccgca tctggaaatt tcccggttgg
    12181 catctctgac ctcgctgata taatcagcaa atctgtatat atacccagct ttttggcgga
    12241 gggcgttgcg cttctccgcc caacctattt ttacgcggcg gtgccaatgg acgtttctta
    12301 cctgctcgac agccttaatg acaaacagcg cgaagcggtg gccgcgccac gcagcaacct
    12361 tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta ctggtgcatc gtatcgcctg
    12421 gttgatgagc gtggaaaact gctcgccgta ttcgattatg gcggtgacgt ttaccaacaa
    12481 agcggcggcg gagatgcgtc atcgtatcgg gcaactgatg ggcactagcc agggcggtat
    12541 gtgggtcggc accttccacg ggctggcgca ccgcctgctg cgtgcgcacc atatggacgc
    12601 caacctgccg caggatttcc agatcctcga cagtgaagac cagctacgtc tgcttaagcg
    12661 tttgatcaag gcgatgaatc tcgacgagaa gcagtggccg ccgcgtcagg caatgtggta
    12721 catcaacagc cagaaagatg aggggctgcg tccgcatcat attcagagct acggtaatcc
    12781 ggtggagcag acctggcaga aggtgtatca ggcgtatcag gaagcgtgtg accgcgcggg
    12841 cctggtggac ttcgccgagc tgctgctacg tgctcacgag ttgtggctta acaagccgca
    12901 tatccttcag cactatcgcg aacggtttac caatatcctg gtggacgaat tccaggatac
    12961 caacaacatt cagtatgcgt ggatccgcct gctggcgggc gataccggca aagtgatgat
    13021 cgttggcgat gacgatcagt caatctacgg ctggcgcgga gcgcaggtgg agaatattca
    13081 gcgtttcctc aatgatttcc ccggtgctga aactatccgt ctggagcaga actaccgctc
    13141 taccagcaat attctgagcg ccgcgaacgc cctgattgaa aacaacaacg ggcgtttggg
    13201 taaaaaactg tggaccgatg gcgcggacgg tgagccgatt tccctttatt gcgcttttaa
    13261 cgaactcgac gaagcgcgtt ttgtggttaa tcgcatcaaa acctggcagg acaacggcgg
    13321 ggcgctcgct gagtgcgcca ttctctaccg cagcaacgcc cagtcgcgtg tgctggaaga
    13381 ggcgttattg caggcgagta tgccgtatcg tatttacggc gggatgcgct tcttcgaacg
    13441 ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt gccaaccgca acgacgacgc
    13501 ggcctttgag cgtgtggtga atacgccaac gcggggtatt ggtgaccgga cgctggacgt
    13561 ggtacgtcag acatcgcgag atcgccagtt aacactctgg caggcatgtc gtgaactgtt
    13621 gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta cagcggttta tggagctgat
    13681 cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat gtacagactg accgggtaat
    13741 taaagactcc ggcctgcgca ccatgtacga gcaggagaag ggcgaaaaag gtcagacgcg
    13801 tatcgaaaac ttagaggaac tggtgacggc aacgcgccag ttcagctaca acgaagaaga
    13861 cgaagattta atgccgctgc aggcattcct ctcccatgcg gcgctggaag cgggcgaggg
    13921 gcaggcggat acctggcagg acgcggtgca gttgatgacg ctacactcgg cgaaaggtct
    13981 ggaattcccg caggtgttta tcgttggtat ggaagagggc atgttcccaa gccagatgtc
    14041 gctagatgaa ggcgggcgtc tggaagaaga acgccgtctg gcctacgttg gcgtaacccg
    14101 cgcgatgcag aaactgacgc tgacctacgc ggaaacccgc cgtctatatg gcaaagaggt
    14161 ttaccatcgc ccgtcgcgct ttatcggtga gttgccggaa gagtgtgtgg aagaggtgcg
    14221 cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgt atggggacgc cgatggtcga
    14281 gaacgacagc ggctacaagc tcggccagcg cgtacgccac gctaagtttg gtgaaggcac
    14341 catcgtcaat atggaaggca gcggcgagca tagccgtttg caggtggcat tccagggcca
    14401 gggtattaaa tggctggtgg cggcttacgc ccgactggag acggtgtaat ttacgattcc
    14461 gttgccggat gcggtgcgct acaccgcatc cggcatcagc accttactta gcgcacttct
    14521 tcacatcaat attccagtca gcaccagacg caacctgatg tttctgcgcg aaattatcca
    14581 tattcagcgc cacggaaaca ttcaataagc tgttcagata caccatcggc tggccttccg
    14641 gcacatcgcc gaagcttgcg acatacggca ttttccctgc gtatttttgc tgggaacctt
    14701 cggaaatggt cacacacaac gtgtcgttga gtttgattcc tgcctgattc agcaactcgt
    14761 cgctgatatt gctccagacg ttgccgtact gaatatcgag aatcgggata ttgcccttca
    14821 cttcaccttt tgttgctttg gctttctgat aagaaagttc gacaactttt gcaggaagct
    14881 ctggccccac ctgctcgaac gtgatcgccc cggaagccag acgcgcaccg gtgtaggcat
    14941 atacatcacg accatggaag gtatatgact tttcggaacc tttcagacgg ttggtttttt
    15001 catcgatttg ccggacgctc tcaataccca gcgattccgc caccaacgtg agcgtaccgt
    15061 tatctggtga aacgaagtac tgaccgtttt tggttttcag cactaccgat ttacggtctg
    15121 taccaacacc tggatcgacc acgctaacaa acaccgaacc ttgcggccag taactggcag
    15181 tctgatacag gcgataagcg ccttcccaga tgttatacgg tggaatttcg tgtgttaaat
    15241 caaagatttt aagattatgg tcaacaccga atgcgacccc tttcatcgcc gagacggcac
    15301 catctttcag gctaaaatcc gtttgtaaaa tcaaagcgtt atccgcataa gcggatgatg
    15361 accccagaca ggttaataaa aataatgttg cgagcgtttt tttcattttt gtttcccttc
    15421 atttatctga tgattttcca gctgtgtagc cagccccagt ttgtctggcc tcctcgagtg
    15481 tacaatgttt aacctttctg ctcgacggaa atcatggtga caaaaggata gcgttgccac
    15541 gggattgcct cgcctttcat atacatatgt gaaattgtgc catcaagata aagcagctgc
    15601 tcaacgttca gtttcgcttt ggcataacag gcaaaatcat aaaaatttgt cgcctgctgg
    15661 ctcagcaaaa acacggcgtt cccatgttta ttaatcccaa caccgttacg aattttacgt
    15721 gaggcgacgt tgggatgaat acgcggatta attacaccgt tttccatcaa caatggccct
    15781 gactgcaccg caaactgaat ctctttactg gttttaaagg catccagacg aacgatgccg
    15841 actttatctc ccgcgacata aaacacgccg ccagggcgaa taaagaaatt cccttcacct
    15901 gaagcgagat ttaacgccac cttctgctga ccgttttcga tgtacaaacc gagcggcgca
    15961 tagctttcat catagatgcc gccgttcatc gccatctgca cctgaccctg actattaata
    16021 tccgccagca gcgcatgtaa cgttccccac gcttcgccat tggctttttg ccagtacatt
    16081 ttcacccgct ctgtttgagg attaacggta tacgcctgta cggtcagcgt cggatctgag
    16141 ggtgcgcaat catcagcggc aacagcaaac aagggaagta aggtgagggt gagaaaaatc
    16201 cgtttgagat tcaaggtgat cattccttta ccaatgaata gccgatgcgc cattataggc
    16261 cctgaatgtg ggattttttt atcctgttag cgaccttgac gagtaccaaa aagcgcgaag
    16321 ttcaactatt gttctgtggt gttctgttgc gtgttgacgg caaaattttg ctggcgtaac
    16381 atgcgcgcac gatcactcta agaggacatt cgccttggac acacccagta gatactggct
    16441 cactatcctg tcatccagga tcaactccta aggctatccc tttttgctga tagccttagc
    16501 ggttgtcagc gacctcaatt tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa
    16561 cccaatttgt ttctgtgtgc ccaccgaact gtccgatatt ttaagcattg ggagtcccgg
    16621 tcatgctgag cgcatttcaa ctggaaaata accgactgac ccggctggaa gtcgaagagt
    16681 cacaacccct tgtaaatgca gtatggattg atcttgtcga accggacgac gacgagcgac
    16741 tgcgcgtaca atctgaactt ggccagagcc tggcaacccg cccggaactg gaagacatcg
    16801 aagcatcggc acgtttcttt gaagacgatg acggcctgca tattcactcc ttcttcttct
    16861 ttgaagatgc ggaagatcac gccggtaact ccactgtggc atttaccatc cgtgatggtc
    16921 gtctgtttac tctgcgtgag cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc
    16981 gtagccagtc aatggtggac ggtaacgcct acgagctgct gctggatctg ttcgaaacca
    17041 aaatcgaaca gttggcagat gaaattgaaa atatctacag cgacctggag cagttgagcc
    17101 gggtaattat ggaagggcat cagggcgatg agtacgacga ggcgctctcc acgctggcgg
    17161 aactggaaga tatcggctgg aaagttcgcc tgtgtctgat ggatacccag cgcgcgctca
    17221 acttcctggt acgtaaagca cgcttaccgg gtgggcaact ggagcaggcg cgtgaaatcc
    17281 tgcgagatat cgaatccctg ctgccgcata acgaatccct gttccagaag gtgaacttcc
    17341 tgatgcaggc ggcgatgggc tttatcaata tcgagcagaa ccgcatcatc aaaatcttct
    17401 cggtggtatc cgtggtgttc ctgccaccga cgctggtcgc ttccagctac gggatgaact
    17461 ttgagtttat gccggaactg aagtggagct tcggctaccc aggcgcgatt atctttatga
    17521 tcctcgcggg cctggcaccg tatctgtact ttaagcggaa gaactggctg taaaaaacga
    17581 gaacggtggc ttagcctggc taagccaccg attatttaaa ggctccaggt atgaatacct
    17641 tttacttctt tttcatagaa ccatctgttg gtattgtatg aaacataagc ggcgataatg
    17701 aatccaaata cattatagcc aatcatgtaa ccaaaaatta aagaaaatat cgccaggaat
    17761 agaacaacaa tagccttctt ccataaaccc aatacaaata aatagaccca cga
//
LOCUS       DAJOTB010000098        17763 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJOTB010000098 DAJOTB010000000
VERSION     DAJOTB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.098"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            46..1389
                     /locus_tag="OHN28_003241"
     CDS             46..1389
                     /locus_tag="OHN28_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2298579.1"
                     /translation="MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQM
                     NYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPI
                     NGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPF
                     LVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRL
                     ASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
                     LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHR
                     VGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLT
                     GFSNRYDVPEQMTISIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1570..3060
                     /locus_tag="OHN28_003242"
     CDS             1570..3060
                     /locus_tag="OHN28_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2298580.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3103..3606
                     /gene="yeaK"
                     /locus_tag="OHN28_003243"
     CDS             3103..3606
                     /gene="yeaK"
                     /locus_tag="OHN28_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCQ2298581.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3607..3711)
                     /locus_tag="OHN28_003244"
     CDS             complement(3607..3711)
                     /locus_tag="OHN28_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298582.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3881..4327
                     /locus_tag="OHN28_003245"
     CDS             3881..4327
                     /locus_tag="OHN28_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCQ2298583.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4284..5105)
                     /gene="nimR"
                     /locus_tag="OHN28_003246"
     CDS             complement(4284..5105)
                     /gene="nimR"
                     /locus_tag="OHN28_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCQ2298584.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPGSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5202..6383
                     /gene="nimT"
                     /locus_tag="OHN28_003247"
     CDS             5202..6383
                     /gene="nimT"
                     /locus_tag="OHN28_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCQ2298585.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6438..6785
                     /locus_tag="OHN28_003248"
     CDS             6438..6785
                     /locus_tag="OHN28_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCQ2298586.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6807..7061)
                     /gene="yoaF"
                     /locus_tag="OHN28_003249"
     CDS             complement(6807..7061)
                     /gene="yoaF"
                     /locus_tag="OHN28_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCQ2298587.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7244..8269
                     /gene="dgcP"
                     /locus_tag="OHN28_003250"
     CDS             7244..8269
                     /gene="dgcP"
                     /locus_tag="OHN28_003250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCQ2298588.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            8302..8400
                     /locus_tag="OHN28_003251"
     CDS             8302..8400
                     /locus_tag="OHN28_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCQ2298589.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8403..8477
                     /gene="yoaJ"
                     /locus_tag="OHN28_003252"
     CDS             8403..8477
                     /gene="yoaJ"
                     /locus_tag="OHN28_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCQ2298590.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8536..8784)
                     /locus_tag="OHN28_003253"
     CDS             complement(8536..8784)
                     /locus_tag="OHN28_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2298591.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8932..9114)
                     /locus_tag="OHN28_003254"
     CDS             complement(8932..9114)
                     /locus_tag="OHN28_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCQ2298592.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9118..9477)
                     /gene="yeaR"
                     /locus_tag="OHN28_003255"
     CDS             complement(9118..9477)
                     /gene="yeaR"
                     /locus_tag="OHN28_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCQ2298593.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9650..10288)
                     /gene="leuE"
                     /locus_tag="OHN28_003256"
     CDS             complement(9650..10288)
                     /gene="leuE"
                     /locus_tag="OHN28_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCQ2298594.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10415..10747)
                     /locus_tag="OHN28_003257"
     CDS             complement(10415..10747)
                     /locus_tag="OHN28_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2298595.1"
                     /translation="MSLTPNALDTTTSKYRFLNDDFILCSFIHSTAWRGIFAFGVFPD
                     HVKIDVARLFPASGHLVQVLPEYYQPANVWAVYVSKLSTSAKVRITVEFLRQYFAEHY
                     PNFSLEHA"
     gene            complement(10831..11946)
                     /gene="rnd"
                     /locus_tag="OHN28_003258"
     CDS             complement(10831..11946)
                     /gene="rnd"
                     /locus_tag="OHN28_003258"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCQ2298596.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(12028..13713)
                     /gene="fadD"
                     /locus_tag="OHN28_003259"
     CDS             complement(12028..13713)
                     /gene="fadD"
                     /locus_tag="OHN28_003259"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCQ2298597.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13918..14499)
                     /gene="yeaY"
                     /locus_tag="OHN28_003260"
     CDS             complement(13918..14499)
                     /gene="yeaY"
                     /locus_tag="OHN28_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCQ2298598.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14539..15234)
                     /gene="tsaB"
                     /locus_tag="OHN28_003261"
     CDS             complement(14539..15234)
                     /gene="tsaB"
                     /locus_tag="OHN28_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCQ2298599.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(15292..17202)
                     /locus_tag="OHN28_003262"
     CDS             complement(15292..17202)
                     /locus_tag="OHN28_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCQ2298600.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            17334..17678
                     /locus_tag="OHN28_003263"
     CDS             17334..17678
                     /locus_tag="OHN28_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2298601.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4409 a         4492 c         4242 g         4620 t
ORIGIN      
        1 tctctgccat caataaaatc cagcactcac attgctctcc tttttatggc ttctatgggt
       61 acacaaaaat taaaagctca aggctttttt attttcagct tattgctgac gttaatttta
      121 ttttgcatta ctaccttata taacgaaaac acgaatgtaa aactcatccc acagatgaat
      181 tacctgatgg ttgttgtggc tttgtttttc cttaacgccg tcatttttct tttcatgtta
      241 atgaagtatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta
      301 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat caacggcagt
      361 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
      421 ttcatttgtt taacctcgct ggcgctcttt tgttatggga aagacaacat ccttgacaac
      481 aataagaaaa aaactggcat tctgttgctg gcgctgattc cttttttagt ttttcccctt
      541 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgattactgc
      601 ccggacaacc atactgcgac ctggggaatc aactatacaa aaatattggt ttgtttgtgg
      661 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
      721 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag
      781 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
      841 gtgtcttttc tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt
      901 catgatgtgc tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
      961 ttgtcgcgac ctgttgttaa ggacttttgt gtcatgctgg ttgatattaa tcagttcaaa
     1021 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtatc aattgtcgat
     1081 attatccagc aaagcatccg ccccgatgat attttagcgc gactggaggg tgaggtgttt
     1141 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt
     1201 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc
     1261 atcagtattg gcacggtttt ttcaacgggt gacacgcata atatctcgct tgtcatgacg
     1321 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
     1381 catatttaag tgcaaaaata ttcacagcca tgctttttgc gtggcttttg catacaattt
     1441 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
     1501 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg
     1561 ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt
     1621 gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc
     1681 tatctgcgct atatcgtgca gagagcagac tcttcctttc tttatgataa gtatcagaat
     1741 cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtaccgcca
     1801 gaacaacggc gcgctatctg cgaggctttt gagtctgcca ataataccca tggcttaaac
     1861 ctgactgctc ataaatacca gggcttacgc ggcacactac aaaccgcatc cactgactgc
     1921 gacacaattg tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac
     1981 cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat
     2041 ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg
     2101 atgaataact ggtccttcct gcaatcagga agtattggta tcgatcgcaa agatattgaa
     2161 aaagtattta ccgggcgtac cgtattatcg agcatttacc aggatcagcg tactaaacag
     2221 aacgtgatga gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg
     2281 ctggatatta acaaaaacaa tctgcggaat atcttttaca ctcatgaccg ccctctcctc
     2341 tggcgttttc tcaatgtcac gctaaccgat acagattcgg ggcgcgacat tatcatcaac
     2401 cagagcgaag ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt
     2461 cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg
     2521 ttctggattt tgacggcgtt aattttgctg aatatggtgc ggatgcactt ccgcttatac
     2581 caaaatgtgt cgcgagaaaa tattagtgat gcgatgactg gactctataa tcgcaaaatt
     2641 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
     2701 tttattgcta ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
     2761 ggggatttag cgattacgtt attagctcag gcgattaaac agtcgattcg taaaagtgat
     2821 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
     2881 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
     2941 gagatcggct tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg
     3001 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
     3061 taaccttctg tggttgtttg cttgtaatct caggagcatg aaatgactga aatggctaaa
     3121 ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc taacttccgc
     3181 gtcgttaccc atgaagcggt aggtaaatgt gaagcagtgt cagaaattcg tggcaccgct
     3241 ttaggccagg gggctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
     3301 gttctggcga tcctcgctgc cgatcaacag gccgatctga gccaactcgc cagccatata
     3361 ggcggattac gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc
     3421 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgacccccta
     3481 ctcttcgaac gatttgatga aatcgccttc aatgcaggca tgctggataa atccgttatt
     3541 ttgaaaaccg cggattattt gcgtattgcg caaccagaac tggtcaattt ccgccgcact
     3601 gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacag cagcaatggc
     3661 aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagtatgccc
     3721 ccgggagata gctgttttca cgttattgtt aacgtacaca aatggcagtt tgatgacagt
     3781 tcgtctgatt ttttatgcaa aaaacgtaaa gattttctac ttcctgcctg cagcaagcgt
     3841 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     3901 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     3961 aattctggtg ttgattattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     4021 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc
     4081 cagcgggacg ctaccgccct caaccctgat tcactcgttt ctgaactgga aatcgctggt
     4141 ggcaattgca gtcggcgtta ttgtctcctg gctggggggg cgcggcgtga cgttaatggg
     4201 cagccagccg caactggtcg ccgggctgct ggtgggcact gttttaggcg tggcgctgtt
     4261 tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     4321 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     4381 gtaataaagg ccgttgttga atcatacccc agtgtatgag caactttctg tacagtatcg
     4441 cctttcacta atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa
     4501 ctcaaccccg tctctttcac tatcagccgg gcaagattgc gttcactcat cgcaaaaaag
     4561 ccagcccatt gcctcaatga gccccactcg acgggctctt tcgccatcat ctccaccatc
     4621 gtgcggattt taggatgaga agaaccgggc aagtgcagct gttgttgtgg ttgctgaggg
     4681 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     4741 gtcctgtttg ccagggttaa aattaattcc cggcataagg gcgatatttt caatgtgcaa
     4801 catgttgtcg gcattgtcac tgcgctgggc tcgataaaca gaaaacagag ttcggcgtta
     4861 gctgtcactt gattactatg tttcaccccg cccggtatcc aaaccgcata ttgtggcggc
     4921 accatccaca aagcattttc taccgtacag gtaattgcac catgtagtgc cagtatcaac
     4981 tgccctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     5041 aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc
     5101 atcattttgt ccgaatttag cgatattttg tcattttagc ttgattcaac ataacaataa
     5161 aaacggtaag gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat
     5221 taagcggtaa aaacaggatt ttccttatcg ctggcattct gatgattgcc acaacattac
     5281 gcgtcacctt taccggcgca gcaccgttac tggatacgat tcgttccgct tactcgctaa
     5341 cgacggcgca aaccggctta ttgaccaccc tgccattatt agcctttgcg ctgatctcac
     5401 cgctggccgc cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac
     5461 ttttgatctg tgcaggtatc gcaattcgct ctctcccttc gccttactta ttatttggcg
     5521 gtacagcggt cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta
     5581 aacgcgattt ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag
     5641 ctgcggcggc actgggatcg gcgatggtag tgccgctggc tttgaacggt tttggctggc
     5701 aaggcgcgtt gctcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac
     5761 agtggcgaag tcaacaacat gcaaatttga gtacctcacg cgccttacat actcggggta
     5821 tctggcgttc gccgcttgcc tggcaggtaa cattgtttct tgggatcaac tcactggtct
     5881 attacgtgat tattggctgg cttccggcga tcctcatcag tcaaggctat agcgaagcac
     5941 aggcgggttc actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc
     6001 cacttttctt acatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt
     6061 gcgcagtggg cgcggttggg ctctgcttta tgccagcaca cgcgatcacc tggactctgc
     6121 ttttcggttt tggttccggc gcaacaatga tactggggtt gacgttcatt ggtcttcggg
     6181 caagttctgc gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc
     6241 tggcagcctg tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg
     6301 taccactgat gggtgttgca atactttcac tactgatggc gattttcgga ctttgcgccg
     6361 ggagagacaa agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga
     6421 aagggagaac aaatacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga
     6481 gcgatggtta tcgcgtactg gtcgaccgcc tctggccacg cggtatcaaa aaaaccgatt
     6541 tagcccttga tgagtggaat aaagaaatca cgccgtcaac ggagctgcgc aaagcctttc
     6601 acggcgaagt cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac
     6661 acgagcaaga aggaaagcgg ctggcagaca tcgccaaaaa acagccgctg accctgctct
     6721 actcagcaaa aaacaccacg cagaaccatg cgctggtgct ggcagactgg ctacgtagct
     6781 tgtgatttta gtacagcatc cggcggttat ttttcaccag ccggatggtc ccgtcgccac
     6841 aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc
     6901 gctgtctgca caggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt
     6961 gccataccaa tctgcggcgg tttaggtgct tctggctgag aaggggttga acaaccagcc
     7021 aggaccagca agcaaggcag aacaaaactg ataattttca tttattgatc tcacatattt
     7081 atccaagctt agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca
     7141 ctcaaaacat attacgcctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag
     7201 attaaataat cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat
     7261 cgcccgcgtc tcgcaatccc ttgccaaaga acagtcactg gaaagtctgg ttcgacagct
     7321 tctggagatg ctggaaatgg tcactgatat ggagtcaacc tacctgacca aagtggatgt
     7381 cgaagcgcgc ctgcagcata taatgtttgc ccgtaatagc cagaaaatgc acatcccgga
     7441 gaattttacc gtctcgtggg attactcgtt atgcaaacgc gccattgatg aaaactgttt
     7501 tttcagcgat gaagtccccg accgttgggg cgactgtatt gcggcacgca atcttggcat
     7561 caccacattt ctgagcacgc caattcactt accggatgga tcattctatg gcacgctttg
     7621 cgccgccagc agtgagaagc gccagtggag tgaacgcgcg gaacaggtct tacagttatt
     7681 cgccggactg attgcacaat atattcaaaa agaggcactg gttgaacagc tgcgcgaagc
     7741 taatgctgcg ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg
     7801 ggcgattttt gaaaatctga cgacgctgtt ttctctcgcc cggcatctta accataagat
     7861 aatgatcgcg tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa
     7921 tagtggcgat ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg
     7981 cgaagttatt ggtcgtctcg gcggtgatga gtttttggtt gtttcactga acaacgagaa
     8041 tgcggatatt tcgtcgctgc gagaacgtat tcaacaacaa atacgcggag aatatcactt
     8101 aggtgatgtt gatttgtatt atcctggtgc cagtcttggc atagtagaag tcgatcctga
     8161 aacgaccgat gcagacagtg ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa
     8221 acataaacag aaaacacctt ttgtcacgca ttcagcgcta cattcctgag gcgtattcac
     8281 atccttttga ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt
     8341 acagcggtcg tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa
     8401 agatgaaaaa aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg
     8461 agctgggatg gtggtaacgt cacatctaaa aaatagcaaa ggctgcctgt gtgcagcctt
     8521 tgtgcaattt acgcgttaac ttttaatctt cctgtagata aatagcacaa caatcgcacc
     8581 aataacggca accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag
     8641 cgtgctgatc catccgccga ctacggcacc gactatcccc agcaggatag tcataaagaa
     8701 tccaccacca tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa
     8761 aataatccat gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta
     8821 gtcgctgata aaaagcatag cacaacatcg ggaggggaag atttgtgacg agcatcacgg
     8881 aggaattttt tgcgatggcg cagaaattgc gccatcaacg atcagtgata attaccaacc
     8941 acaaacatca tgttcgtttt ccgtgtcata agaacgcacg gtattcacca gatcttttat
     9001 cacttcagcc gccacttctg gcaccagcaa agtcatcggc gtctctgttt cataatcgac
     9061 agaaacgcca ttgctgttat tggtgacggt cacggtatac gttgctttgc ccatgattca
     9121 tttcccgtta tgaatgactt tccgttgttg cgcgccttcc atcaggactt caggagcaac
     9181 gaagaagtca atgttgaaat aagtatcgtc agtcatggct tcaatgttgt gccacttttc
     9241 tggagggaac accgcaaact gccccgcttc gataaggatc acctgatcag gctctgcact
     9301 gtgttcatca gcgtagccga gatatttgac cgccccatgc ataacggaaa ggcgtgggta
     9361 aacccccggg cgcgttcctt tatcaagatg acgttcgaat attccggcag gcgcagtttg
     9421 tttattccag aaaggcgttg agcgcgtatg aatataattc tgtgggattt gcagcatcct
     9481 tttccctcct tcggtgaatg cgctggaaac ggtttattcc agccgtttca gggtacgcct
     9541 gataatttgc attttaaata ccatttattg gttacttttt agcaccgtat cagagaagaa
     9601 tcagggagga ttacaggtgg gaagcccgcg gcaacgcggg ctgaaagcat caggattgca
     9661 gcgtcgccag tcgggcggcg aaacccacga acatcaaacc aatcagtgag ttgcccactt
     9721 tagccagttt ctttttggta cgtatgtact gcgtgacaaa agccccagaa ataatcagga
     9781 agctcaaata gcagaaactc accagttcca gcgtcgtcgc cagaataaag aatgaaattc
     9841 ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa cgacacatag aacaaaatgg
     9901 ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa aatggcaccg tattggggct
     9961 catcggattt ggtctcgcta tttttacctt tcagggtcgc gtagagaatt ttactcccca
    10021 gatagagcaa ataaaacgca ccaagataac gtacgatgtt gaataatatc ggggtggtct
    10081 taattaatgt cgccactcca gcccatgcca gaaacatcaa taccgcatcg ccaataaata
    10141 caccacaggc cgcaagataa ccgcctttca taccgctact gacgctattt ttgagtacaa
    10201 acagggtatt tggccctggc accaacacaa taaaaatggc cccaaccaga taggtccagt
    10261 aattcagaac cccgtattca gcgaacacat taacctcttt aattatcttt cgatcatgcg
    10321 cgattaaagg tgaatatgct aaccaatctg tagcggctta gaaaggagaa aatcaggttt
    10381 taactcgata tcaacccgat aattgaatca ttaatcaggc atgctccagt gaaaaattcg
    10441 ggtagtgctc ggcaaaatac tggcgtaaaa attctaccgt tatccgcact ttcgctgacg
    10501 tcgacagctt tgaaacataa acggcccaga cattcgctgg ctggtaatat tccggtagca
    10561 cttgtactaa atgaccactg gcgggaaaca gcctggcgac atcgattttt acgtggtccg
    10621 gaaaaacacc gaaggcgaat attcctcgcc aggcggtaga gtgaatgaag gaacagagaa
    10681 tgaagtcgtc attcaggaat cggtatttac tcgtcgtggt gtcgagcgca tttggcgtta
    10741 agctcaaata agttcatttg aaccgcctgc tgttggattt gtagtttgcc gaagccggac
    10801 tgagcgcgcc cagtccggct tcggaagatt ttactgtgga tattcctgca ataaattgtg
    10861 taatgcttcc gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg
    10921 cggtttcagt ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa
    10981 ttcggcgctg atcttgtgcg tttcgctgac gtcagtaatc agcgacttaa tcgctttaaa
    11041 cgctttacga taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc
    11101 cggcaatgcc tgcgcttttt ccaccagcgc cagcaacgtt ttaccgtgga agcggatttc
    11161 gctaccggat aagcccaggc tgtccagttc gcccagactt cccggcatat aacgcgctac
    11221 cgaccacaaa tgctcttcac gcacgacaaa gttcaccgcc agatcgcgct ctcgcgcctt
    11281 gcgcaatcgc cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaactgcca
    11341 ggcattggtg atatcacgcc aggcgtcttc cggcgcaacg acttcctgac gacgcatttg
    11401 catcaggcgg cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat
    11461 aagctttgcg gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg
    11521 ttcggtcagc ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc
    11581 ggaatactct tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc
    11641 aaggatttgc gtgtcaatca agggttgtgg taattcgcca aagacattga ggaacacttc
    11701 cagatcttca ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt
    11761 cagcggtgac cagtcggtga tcccgagtgg atcgattagc gccagatgct caccatcaaa
    11821 aagttgaatt aaacccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag
    11881 ggctatcgcc ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt
    11941 aatcatttgg taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta
    12001 accggcgtct gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc
    12061 gtcacgtaat tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa
    12121 ctccaccagc ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga
    12181 ctcttcggta agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga
    12241 gccggaaggt acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc
    12301 ttcaatctcg ttgggataga cgttaaaacc ggaaaccaga atcatatctt ttttacgatc
    12361 gacaatgcgc aggaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc
    12421 atttttgatg atttcatcgg tagcatccgg acgctgccag taacccagca tcacctgcgg
    12481 tcctttgaca caaagctcac ctggttgacc aggtggtact tcattatcat catcatccac
    12541 cagtttggct tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc
    12601 atacgggtta acgctgacca gcggcgcgca ctcggtcagg ccataacctt ccagcagata
    12661 ctgtccagtc agtttaaccc aacgttctgc aaccacttgc tgaacaggca tcccaccgcc
    12721 tgcggaaaga tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc
    12781 attgaacaag gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac
    12841 cagccctgga atatcgcgcg ggttagtgat aagcaggttc tgccctccca gttcgataaa
    12901 cagcaggcag ttaatggtca gtgcaaaaat gtgatacagc ggcagcgccg tcaccaccag
    12961 ctctttgccc ggatgcaaca gcggaccata ggtcgcgtta acctgttcca ggttcgccag
    13021 catattgcgg tgagtcagca tcgcgccttt cgccacacca gtggtgccgc cggtgtattg
    13081 caggaaagct aaatcttccg gcaccaattc tggtttgacg tactgcatcc ggtagccgtt
    13141 gtgcagggcg ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccagacgctt
    13201 gatgtattta acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat
    13261 acgggtcaga ataacgtgct gaacggcggt tttatcaacc actttttcca gagtgtgggc
    13321 aaagttagac acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc
    13381 acgcggggta tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa
    13441 cagcgccacc ggatattgca ataagttagg catcatcaac gcaacgcgat cgcctttctt
    13501 cagccccaac ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagtttgcg
    13561 gaaggtcatc acctccccca tattcacaaa cgcgggttga tcggcatagc gtgcgaccga
    13621 ctgctcaaac atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc
    13681 cgcgggataa cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt
    13741 cgtcatcgca accccaagtg ataaacatgc tattaacata atattaactc atcttaccag
    13801 cttgataatt acccaaggaa aaggttgcga agcgcgtcac tatttatttt tatctttacc
    13861 gtaagaatgc agaaacagcg gaccggccgc tgtttctttt tctttaaaaa caagcaatta
    13921 ttcagttaca actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgta
    13981 gccatagggc cagccacgac cgccataaaa ccatggttca atcggctgag gcggcataat
    14041 cacctgctgg gttaaatgcc aacgtttgta acccgttact tgcatcacca taaatttata
    14101 gggcgtatta ccgattttgc cgtcaaccgc accggtgatt ggcccgacta ccgtcaccag
    14161 ttgtccgcgg aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg
    14221 agaaggttct cccagcgtcg gtctggctcc gctgtccagt ggtacggtgg caatttccag
    14281 gcgggttttc ccttgctggt tttgtacagc aaccactttg ccgccaaagc gagcctcctg
    14341 accaacgtac agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact
    14401 actgccttta atggcgtccg gcacagtaac acaaccgctc agcattagcg caaacgtacc
    14461 tgccagtatg cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc
    14521 agggcatact cttaagattc attctttgcc cggaagtttc ttccatgcga cattgttacg
    14581 taaataaact ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctggca
    14641 cgcaatcggc agcatatctt ctgcagcggg cagtaacact tcgccatcgt gcaaaaccag
    14701 cccgctctct ttaccgagat ccggccaggc ttgccagccc gtccctaccg tcacccattc
    14761 gccggaaagc tgttgcattc gttcatgaac cagttcgggt ttgagtacgg cttcggtttc
    14821 ttcaccgtgc cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat
    14881 tcgcgcgtca atagctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccttgcgc
    14941 catcgtcatt agcgtggaga cgccaatcat cggcagctct gcgccaagtg ccagcccttg
    15001 cgcaatacca ataccaatgc gcacgccggt aaagctaccg gggccgcgcc cgtaagccag
    15061 agcgtttatg tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa
    15121 gattcgttga gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt
    15181 ccacagggcg acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct
    15241 tcgtgcttag atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga
    15301 ggatggtatc gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgcg gcgctggcgg
    15361 cagactggcg agaaacgtcg cgccgtaagg gcgcatcacc agccgattgt cgcaaatcac
    15421 cagcacgccg cgatcgtcgg cgtcgcgaat caatcgccct accccctgct tgagagtaat
    15481 gaccgcatcc ggcagttgta cttcatcgaa cggatcacca ccgcgcaaac ggcaatcttc
    15541 catgcgtgct tttaatagtg gatcatccgg cgaggtaaac ggcaatttgt cgataatcac
    15601 caatgacaat gtatcgccac gcacgtccac cccttcccag aaactgctgg ttgccacaag
    15661 aagcgcgtta ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg
    15721 caacaaaacg ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc
    15781 gtgcgaggta caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat
    15841 tgccgccagt tgccgtgcgg aacccggttg attggtttgc ggcaggttgc gcggcacaca
    15901 gagtaacgcc tggcggctgt aatcaaaggg gctgggtaac agcaatgact ctgcctgttc
    15961 aatgccaagc cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt
    16021 aaagatccag ctaccggttt tttgcgccat taactctttg aatttatccg ccacgctgag
    16081 cggcgtgaga gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg
    16141 ctgattgatc tcttttagcc gtttcagccg tgtgcgatat aacgtggcgc gctcaaatgc
    16201 cgcatccagc aaggcggaac gccccagcga cagtttcgcc acgtcataac aaagttccag
    16261 agtgtcatcg agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag
    16321 gttaccgcga taaccaggct caccgagttg caggcgaaaa tcctgcgcac tctgggcaag
    16381 acgatccgcg cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt
    16441 gatgtctttt gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct
    16501 ggcaatgtcg ggcagctggt gggcttcgtc gaagatcatg acgtccgcct ccgggatcag
    16561 ttcgccaaaa ccgctctctt tcaccaccat atccgccaga aagagatgat ggtttaccac
    16621 caccacatcg gcgtccatcg cttttttgcg tgctttgacc acaaagcaat ctttatacat
    16681 cgggcagtcg ctgccgaggc agttatcgtt ggtgctggtg accagcggcc acgcctgtga
    16741 atcttccgcc acgctgacgc aggtgctgat atcaccatcg attgtttgat tagaccagga
    16801 gcgcagcagg atcacatcgc ttaagatttg taccggcaga tcgccccccg ccaaagcctg
    16861 ctgttcgaga cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacattgcc
    16921 cgtgtatttc aatgcctttg agaccgttgg caaatcgcgg ctgtagagct gatcctgcaa
    16981 cgcttttgag ccggtcgaga ttatgacttt ctttttcgcc cgcagcgcag gggccaggta
    17041 agcgtaggtt ttgcccgtac cggttccggc ttccaccacc agcggctggc ctttttctat
    17101 cgcctgggtg acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat
    17161 cgctttcgcc agctgaccgt cttgtgcaaa atcgtccgtc acactacccc ctgttgattt
    17221 gaacagggat tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcgacctc
    17281 acattgtggc agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta
    17341 tcgttcgtat cgatgccgaa gcccgctggt ctgatgtggt aatccacaac aacacgctct
    17401 actacactgg tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc
    17461 tggcgcagat tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg
    17521 ccaccatttt cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct
    17581 gggttgtcgc gggtcatgcg ccggtgcgct gcacggtaca agcaggtttg atgaacccga
    17641 agtataaagt tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt
    17701 cagacgataa tttatcgaca atactggtcg gttttgcaaa aatcgaccag agattaagat
    17761 att
//
LOCUS       DAJOTB010000099        17328 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJOTB010000099 DAJOTB010000000
VERSION     DAJOTB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.099"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..165)
                     /gene="holE"
                     /locus_tag="OHN28_003264"
     CDS             complement(<1..165)
                     /gene="holE"
                     /locus_tag="OHN28_003264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCQ2298602.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRER"
     gene            304..678
                     /gene="yobA"
                     /locus_tag="OHN28_003265"
     CDS             304..678
                     /gene="yobA"
                     /locus_tag="OHN28_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCQ2298603.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            682..1554
                     /gene="copD"
                     /locus_tag="OHN28_003266"
     CDS             682..1554
                     /gene="copD"
                     /locus_tag="OHN28_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCQ2298604.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            1567..1908
                     /locus_tag="OHN28_003267"
     CDS             1567..1908
                     /locus_tag="OHN28_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCQ2298605.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            2303..2959
                     /gene="pphA"
                     /locus_tag="OHN28_003268"
     CDS             2303..2959
                     /gene="pphA"
                     /locus_tag="OHN28_003268"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2298606.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(2960..3151)
                     /locus_tag="OHN28_003269"
     CDS             complement(2960..3151)
                     /locus_tag="OHN28_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCQ2298607.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(3256..3492)
                     /locus_tag="OHN28_003270"
     CDS             complement(3256..3492)
                     /locus_tag="OHN28_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCQ2298608.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(3609..5048)
                     /gene="rsmF"
                     /locus_tag="OHN28_003271"
     CDS             complement(3609..5048)
                     /gene="rsmF"
                     /locus_tag="OHN28_003271"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCQ2298609.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(5128..7761)
                     /gene="yebT"
                     /locus_tag="OHN28_003272"
     CDS             complement(5128..7761)
                     /gene="yebT"
                     /locus_tag="OHN28_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCQ2298610.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(7730..9013)
                     /gene="yebS"
                     /locus_tag="OHN28_003273"
     CDS             complement(7730..9013)
                     /gene="yebS"
                     /locus_tag="OHN28_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCQ2298611.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9143..9640
                     /gene="msrC"
                     /locus_tag="OHN28_003274"
     CDS             9143..9640
                     /gene="msrC"
                     /locus_tag="OHN28_003274"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCQ2298612.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            9737..10435
                     /gene="proQ"
                     /locus_tag="OHN28_003275"
     CDS             9737..10435
                     /gene="proQ"
                     /locus_tag="OHN28_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCQ2298613.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            10455..12503
                     /gene="prc"
                     /locus_tag="OHN28_003276"
     CDS             10455..12503
                     /gene="prc"
                     /locus_tag="OHN28_003276"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCQ2298614.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            12695..13576
                     /gene="htpX"
                     /locus_tag="OHN28_003277"
     CDS             12695..13576
                     /gene="htpX"
                     /locus_tag="OHN28_003277"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCQ2298615.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(13622..14995)
                     /locus_tag="OHN28_003278"
     CDS             complement(13622..14995)
                     /locus_tag="OHN28_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298616.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            15172..15963
                     /gene="kdgR"
                     /locus_tag="OHN28_003279"
     CDS             15172..15963
                     /gene="kdgR"
                     /locus_tag="OHN28_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCQ2298617.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(16107..16346)
                     /locus_tag="OHN28_003280"
     CDS             complement(16107..16346)
                     /locus_tag="OHN28_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298618.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            16505..16648
                     /gene="mgrB"
                     /locus_tag="OHN28_003281"
     CDS             16505..16648
                     /gene="mgrB"
                     /locus_tag="OHN28_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCQ2298619.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            16723..17010
                     /locus_tag="OHN28_003282"
     CDS             16723..17010
                     /locus_tag="OHN28_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCQ2298620.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         4505 a         4208 c         4401 g         4214 t
ORIGIN      
        1 ccgctcgcga aaccagctgc gcaaatgttc aggctgttca cgttcaaccg cttcagcgat
       61 cactggcata ttgtagcgtt ctttaaatgc cacccctgcc gccgccaaat cgacattcac
      121 tttatccatt tctgtttgat ccagtttagc cagattcttc agcattctta atctcctcat
      181 cattcgcggg caaaagttac taagagtgag ccttgatggc aagccggaat tgatgatcct
      241 cgactcagac attagacaag gttatgctct gtaaaaaagt tattacaaaa aggaatagat
      301 aatatggctt caactgcacg ctcccttcgc tacgcgctgg caatcctgac cacttcgttg
      361 gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc agtatcctgc ggcaaatgca
      421 caagtgacag cagctccgca ggcaatcact ttaaacttct cggaaggcgt tgaaacagga
      481 ttcagtggcg caaaaatcac ggggccaaaa aacgaaaata ttaaaacatt accggcgaag
      541 cgtaatgagc aggaccaaaa gcaactgatt gttccactgg ccgattcgct gaaacccgga
      601 acctacaccg ttgactggca tgttgtttcg gtggatgggc ataaaacgaa aggacattac
      661 acctttagcg tgaaataaat catgctggcg tttacctgga tcgcgctgcg atttatccat
      721 ttcacctcgc tgatgctggt ttttggcttc gcaatgtacg gtgcctggct ggccccctta
      781 acgattcgtc gcttgctgac gaagcgtttt ctgcgtttac aacagcacgc tgccgtctgg
      841 agtttgatca gtgccactgc aatgctcgcc gttcagggcg ggttgatggg aacgggatgg
      901 tcagatgtat tttcgccaaa catttggctg gctgttttgc aaacgcagtt tggtggcgtc
      961 tggttatggc aaattgttct cgccctcgta acattgatcg tcgcccttat gcaaccgcag
     1021 aatatgccac gtttgctatt tatgctcact accgcgcaat ttattctgct ggcgggagtc
     1081 ggtcatgcga cgctgaatga aggggtaacg gcgaaaatcc atcagactaa tcatgcaatt
     1141 cacctgatct gtgcggcggc gtggtttggg ggattactgc cggtgctctg gtgtatgcaa
     1201 ctcatcaaag gccgttggcg acaccaggct attcaggcgc tgatgcgttt ttcctggtgc
     1261 gggcattttg cggtgatcgg cgtactggca agcggcgtgc ttaatgcatt gttgattacc
     1321 ggatttcccc ccacgcttac gactcactgg ggccaactct tattgctcaa agccatcctg
     1381 gtgatgatta tggtggtcat cgcgctggct aatcgttatg ttcttgtacc gcgcatgagg
     1441 caggatgaag atcgtgcggc tccctggttt gtctggatga ctaaactcga atgggcgata
     1501 ggtgcggtag tgctggtgat catcagcctg cttgcgactc tcgaaccttt ttgatggatt
     1561 acgaaaatga tgaaaaaaag tattctggcg tttctgttac tcaccagttc tgcagctgcg
     1621 ctggcggcac cgcaggtgat taccgtcagc cgttttgaag taggtaaaga caaatgggcg
     1681 tttaatcgcg aagaggtgat gctgacttgc cgaccgggta atgctttgta tgtcattaac
     1741 ccaagtactc tcgtgcagta tcctttaaac gatatcgcac aaaaggaagt tgccagtggg
     1801 aagactaaag cccaacccat ttcggtgatt cagattgatg atcctaacaa tcccggcgaa
     1861 aaaatgagtc tggcaccgtt tatagagcga gctgaaaaac tctgttaatt acctaaaata
     1921 gccttttgat ttccaataaa aaaccgcctc agttctttca ccagaacggg cggtttttaa
     1981 tatttaagct gatgaccacc acgcttttta ttgaccattt tgcacgcaaa ctggaaaacc
     2041 tggcgtcgtc atctattctt aaagagcaag gcaactaagc ctgcattaat gccaactttt
     2101 agcgcacggc tctctcccaa gagccatttc cctggaccga atacaggaat cgtgttcggt
     2161 ctctttttat ctgttaaaag ccagaagcat tcccttcgct gactttatag tcaaccataa
     2221 cacacactct actgtttgag tccagcgttt tttaacattc ttgttaagat tatgtgatct
     2281 ttagcgcggg aggaaaatat tgatgaaaca gcctgcgccc gtttatcaga gaattgcggg
     2341 tcatcaatgg cgacatatct ggctttcagg cgatatacac ggttgtcttg agcaattgcg
     2401 ccgcaaatta tggcattgtc gttttgatcc gtggcgagat ttacttatct cagtgggaga
     2461 cgttatcgat cgtgggccgc aaagtttacg ttgcctgcag ttactggaac aacattgggt
     2521 tcgtgcggta agaggcaatc atgaacagat ggcgatggat gcgctggcat cccggcagat
     2581 gtctttatgg ttgatgaatg gcggcgactg gtttattgcg ctggcagata atcaacagaa
     2641 acaagcgaaa acggcgctgg aaaaatgtca gcatttgccc tttattcttg aagtacacag
     2701 ccgcaccggc aaacatgtta ttgctcatgc cgattatcca gatgatgttt atgaatggca
     2761 aaaggacgtc gatttgcatc aggtcttgtg gagccgctcg cgattaggtg aacgccaaaa
     2821 agggcaggga attgcaggtg ctgatcattt ctggtttggt catacacctt tgcgccatcg
     2881 cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg gagaactgac
     2941 tcttgtgcaa ttgcaataat taaaaatcac cgtactcctg tgcaggtcgc cagaaaccat
     3001 ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg ctcatagaat
     3061 aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa ccgccatcca
     3121 ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt gccgtaccgt
     3181 taagtttagg agagatttta caacggggga ataacccgga caaataaccc gccatgccga
     3241 cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt gatcgtaacg
     3301 ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg tttcagcatc
     3361 ccaggcatcc agttgcatac ataaatcggg gtctgattta caaggaatgg ttaacgttcg
     3421 atcacccggc gattcgccgt gtaattcgcc gtcgtcgatt tcaaaagcgc ctatgcgcac
     3481 actggttttc atcttcgtgc tctccattta gctgccggtt gtggtacgac cagtccgatc
     3541 gcaccatttt tcagcctagt tgaggcagtc gaatgtgcca gtcaaaaagt gcgctttttt
     3601 gtgcgctgtc aggcgttacc ggtaaaaagt ttcccgtcgc gcaccagttc acgcgggtag
     3661 ctgtttttca gtcgtgaacc aatccgtttg gctaaaccaa tcggctgatg ctggaaagta
     3721 accaatacat catccgccac tggcgcggca tgcgggtata catcgcgccc gcgataccac
     3781 tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcggggga ggcaagggca
     3841 ataacggcct catgctgcca gcgataacct ttgttgtgcg tttcggcaag tttaatcccc
     3901 aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa caaccacagt
     3961 tctttgtcac gctgccagag tcgcaggttt tcatcccagt ttaagccaac acttgcagcc
     4021 gcctgacgaa tttgaccggc ttcgcgatct ttcactgggc taaacgggaa attgcccact
     4081 ttgtattttg gggcgggtaa gacggggatc gcctgagttt tacgcagacg agcaacgaag
     4141 aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc ggtcagcgcc
     4201 ttgtttgcac cagggaagag atcgccaagc ggtaaaaact ctactgcgtc ggggtaagtc
     4261 tctttcagcc acaggcaaac ggcttcgttt tcttcccggt ttaaggtaca ggtcgagtaa
     4321 accagcgtac cgccaggacg taatgcatga aaggcgctgt cgatcagctc ccgttgggtc
     4381 gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg atctttacgc
     4441 accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcgaacat ttctggcact
     4501 gccgcaccaa atacgcggcc atcaaaatgc gtgagcgcaa cattactgat gccacagcgg
     4561 ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc aaggattgcc
     4621 ccttcgttat tcatccgcgc ggcaatttgc gtcgttttgg aacctggtgc agcagcgaca
     4681 tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcaacagg caacattgaa
     4741 ctggcttcct gaatataaaa caggccgctt aaatgctcgg cggtactacc caacggcaat
     4801 gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat tggcgtaagc
     4861 gtccagccat aaggagcggt taattgcagg aaatcagcaa cggaggtttt cagcgtatta
     4921 acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc aaaagagagc
     4981 gtcgaaggca tcgcttcgcg catttgtgtc agaaaggcgt ccgggaaata aacggtgtgt
     5041 tgggccacga gcatatacca ctggcaaaaa aacaggcgtg cagtttagca taaacgctcc
     5101 ggcgcaggca cgccggagca gtgggcatta tttgggaagc gcagttcccc attcacgcca
     5161 ctctttcggt tcactttcct gcaacaggaa gtgcttgcct tcctgggctt tcggtgccag
     5221 tggcgtaccc ggaggcgtgg cgaaggcgat gccgccacgg ataaactggt taaaggtgcc
     5281 ggttttcact acgccgcccg tcagaccaaa gtccagactg taacccgatg ccaaccagaa
     5341 gacggaattg ttacgcacca ggtgttgata gcgtttactg atgcgcatcg caatcatcac
     5401 gcgatctgac aatgtcccca gcgtcattcc ggtaaccgta cctacttcca gaccacggaa
     5461 cagcacaggt gtaccgatac ctaacgaacc ggcttccggc gcttcaacaa taatgcttaa
     5521 gccatccagg taacgcgaat cagtaatggt ggcctcttgt aattcaaagt cgcggcgagg
     5581 attgccccgg cctggttcga cgttgatata cggctggagg atagtatcaa gatgctcaac
     5641 gccagctgcc gaaatttgcg gtgtgaccac tgagaagcgc gtaccaccgc gagcaaaggt
     5701 ctggacatat tccggataga gcaccgcctt tgcctgtact tcattgcgcg cggtaatcag
     5761 atccagcgtc tggatttgcc cgatatcaat accgagatag cgaattggca tcccgaccgc
     5821 cagttttccg gcatcgaaag cgtgaagcgt aatttgcccg ccaaccgcac gggccgctgt
     5881 ttcggaagca tacagaatac gcttgtcgcc tttacgctga ctggcgctgg caccgctgag
     5941 gttatcgaag ctaatagccc cctttaatgc tctggagagc ggggatgcct gtacggtcag
     6001 gccactacca ttcagctgaa ctttcgcccc accttctgcc cagaacacgc tgttgctggt
     6061 cagaaggttg cgatactccg gtttaatatg cagatcgata tcaaacgcgt tagctcgcgg
     6121 acgcacagta ataacttcac caacttcaaa tttacggtac agcactaccg atcctgcctg
     6181 cacatccggc agcgtctctg cactcaaact cacggttgtg gtgggtaaat cgctaaggct
     6241 gttctccagc gctttttcca gattggcata cagtggatag ctggctttca tctcgccttt
     6301 atcgcccggc agaatacgta ttccgccatt gatccattct gaggcgctgg caccgagaaa
     6361 ctcaacgcca tccagcccca ccttcacgtc gacacggctg ttgacgacaa atttgctatc
     6421 gccttttacc agttcgcgat gctgaggctc gatggcgacg gtaaaggtga cgcctttgct
     6481 ggtgagttta cgatcgataa cctggcctac ctgcacgccg tgaagaatga gcggctgacc
     6541 agcatcaata ccgtaacttt ctggtgctgt cagggtcagt gtcagaacat ccggctcttg
     6601 cagcagggct ttttcgcctg gcacaaccac gaactcctta cgtggctcgc catcgccggg
     6661 caccagttcg aaggttttgc cggtcagcag ggcactgaga ttagcatcgc taagggataa
     6721 ttttggatta cgtaattcga tgcgggtatt ttcacgaagc agggtgacga cgctgggatc
     6781 aacggtcatt tccccagtga ctttaccacc aggatttaaa tccagtttag tcagctgtcc
     6841 gacttccagc ccctgataca ttaacggcgt cgagtcggcg gttaatccgg caccgcctgg
     6901 cagttccagt tttattatta cgccacgttg gctatgggcc aggtcttcat acagaccaaa
     6961 ggtatcttcc gcttcggcag gtttcgactc ttcaggtgaa tcgaaggcaa tcgcaccgtt
     7021 aaccagtgcc gccagacttt ccagtttcac cttcgcgcca ctgatactga cgttggcatc
     7081 aacgccggaa acgttccaga aacggctacc ttttttcacc agatcggtaa aacgccgctc
     7141 gatcaggaca tcaatcacca cgccttgctt gttgggattg atggcatagt cgtagacttt
     7201 acccaccggg atcttgcgga aatagaccaa tgaaccactg ctcagtgaac cgagatcggg
     7261 ggcttgcagg tggatcatca gatcgccatt gtccagccga tatttcggtt gggtgtcgag
     7321 tgcgacaaag tgatcctgct ctttaccttt acccggcatc atgccgatat aattaccacc
     7381 gacgagggcg tccagcccgg agacacctgc caacgaggcc tttggcgtca ccaaccagaa
     7441 ctgtgtctct tcgcgcagcg catctttcat atcggacttg atgctgacct tgacttcaat
     7501 cttacgaaga tcgtcgctga ggctgatatc ctgcactgtt ccaacttcaa cgccctgata
     7561 acgaacaggc gtacggcctg gaacaatacc atccgccgac ataaagtcga tggtgacagt
     7621 attgccccgg tcctgataac tgtcccaaat cagccaacca gcaatcatta gcgcgatgaa
     7681 cggcagcagc cagaaaggtg agatacggcg tttattttta atctgcgctt cagtcgtcga
     7741 agcgggcgtt tcctgactca tgtgcatccc aaagtaagcg gctgtccagc cattccacag
     7801 caagaatagt caaaattacc gctgcgccga aataaaacgc agccggtccc atagtaaaag
     7861 cgaggatctg atcgcgatta atcagcgaca tggttaaaga tatgacaaac aggtcgagca
     7921 tcgaccagcg accaatccag gtcaccatcc gcagtaacag aatgcgtgtg cgtaaacctt
     7981 gctggcattt aaaatgaata ctcaacagta aagtgaacat gacgatcact ttagtaaacg
     8041 gtaccagaat actggcgata aacacgattc ccgcaactgc aatgttgcta cttgccagcg
     8101 acataattcc ggaaagaatc gtatcttcct gccgaccacc attcagataa atgatagaga
     8161 taggcaacag gttggcaggt aacaacaaaa cgattgacgc taacagcgcc gcccagcatt
     8221 tttgcagact atgacggcga cgcaggagta gcgggatatg gcaacgcggg cagcgaccac
     8281 gctgatcagg atagccggta aaatggcacc caagacagac acggagtttc tcgtcccttc
     8341 gcgtagcggg gcgctgcgga taaaatcgct cccacagttc ttcgacatta agatgtgaca
     8401 acgtcaccgt cgttaaaatc accaacgcca caaaagagaa caagccgacc cccgcctgga
     8461 tatgggcata atcctgtacc tttatagaag caacgccaat gccgaccagg tagatatcca
     8521 acatcaccca ctctttcagc cgctcaagca tcagcagtac cgggcgtaaa ttcattccca
     8581 atcggttacc aaaccataaa taagctatgg aggtcaccag aatgaggggg gcaccgataa
     8641 cgcagaaaaa gaccatcgcc cccgttatcg catcgccctg tttggtcatt tgccagatgc
     8701 cttgcataac gttggcgtca atacgaatgc ctaacagcca gatatgcaac agcggttcgc
     8761 cccaggcaaa cggcataagc aacagcatgg tgaaggccat tgccgccagg cgcgttagcg
     8821 accagtcgcg cccgtcacga atttttgcct gacagcgcgg acaataggca ctttgatgag
     8881 aatttatctc gggcaggcta aacagcatgt cacattgcgg gcaacgttgg taatcaccac
     8941 gcggcagttc ctcgccgata gccctcactg ttatcttctt tgtcggcgta atttgtggtg
     9001 tgttaagagc catgacgtgc caaatgtaat aagaattaac attaatctta acccatgatg
     9061 ggaatgatct tgagcattaa cgcatttaat gcttatttta ataggctggc agttccaggt
     9121 aagacaacca agtgattata taatgaacaa agcagaattt tacgcggatt taaatcgcga
     9181 ctttaacgcg ctgatggcgg gagaaaccag ttttctggca acgcttgcga acaccagtgc
     9241 gttgttatat gagcgtctca ctgacgtaaa ctgggcaggt ttttatttgc ttgaggacga
     9301 tacactggtg ctcggaccat ttcagggcaa aattgcctgt gtccggatac ccgttgggcg
     9361 cggcgtatgc ggcactgcgg ttgcccgcaa tcaagtgcag cgtatcgagg atgttcacgc
     9421 gtttgacggg catattgcct gtgatgcggc gagtaattct gaaattgttc tgccactggt
     9481 ggtgaaaaat cagattattg gtgttctcga catcgatagt accgtcttcg gtcgctttac
     9541 agacgaggac gagcaaggct tacgtcagct tgtggcacag cttgaaaaag tgcttgcaac
     9601 gacggattac aaaaaattct ttgcgagcgt cgcaggataa tcaacggata acgtagcatt
     9661 tactgatagc gtcattataa tgacgcctgt tcatgcctgc gcttgttggc tacgtccgtt
     9721 gtaatcagga aatttcatgg aaaatcaacc taagttgaat agcagtaaag aagtaatcgc
     9781 gtttctggcc gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc gtccgctgaa
     9841 aatcggtatt tttcaggatt tggtcgatcg tgttgccggg gaaatgaacc tgagcaaaac
     9901 gcaattgcga tccgctttac gtctctacac ttcgagctgg cgttatcttt acggtgttaa
     9961 atccggcgca acgcgtgttg atcttgacgg caacccatgc ggtgagctgg acgagcaaca
    10021 tgtcgagcat gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg cacagcgtgc
    10081 tgaacagcaa gcgaaaaaac gcgaagctgc cgcagctgct ggtgagaaag aagacgcacc
    10141 gcgccgcgaa cgcaagccac gcccgaccac gccacgccgc aaagaaggcg ctgaacgtaa
    10201 acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca gtaaaagcac ctcgcgaaga
    10261 acagcacacc ccggtttctg acatttcagc tctgactgtc ggacaagccc tgaaggtgaa
    10321 agcgggtcaa aacgcgatgg atgccaccgt attagaaatc accaaagacg gcgtccgcgt
    10381 ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt tctgaaacgg
    10441 aggccgggcc aggcatgaac atgtttttta ggcttaccgc gttagctggc ctgcttgcaa
    10501 tagcaggcca gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt ccggtattaa
    10561 aggaagagac gcagcatgcg acggtgagtg agcgcgtaac gtcgcgcttc acccgttctc
    10621 attatcgcca gttcgacctc gatcaggcat tttcggccaa aatctttgac cgctacctga
    10681 atctgctcga ttacagccat aatgtgctgc tggcaagcga tgttgaacag ttcgcgaaaa
    10741 agaaaactga gttaggcgat gaactgcgtt caggtaaact cgacgttttc tacgatctct
    10801 acaatctggc gcaaaagcgc cgttttgaac gttaccagta cgctttgtcg gtactggaaa
    10861 agccgatgga tttcaccggc aacgacactt ataaccttga ccgcagcaaa gcgccctggc
    10921 cgaaaaacga ggctgagttg aacgcgctgt gggacagtaa agtcaaattc gacgagttaa
    10981 gcctgaagct gacaggaaaa acggataagg aaattcgtga aaccctgact cgccgctaca
    11041 aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg ctggcaatga
    11101 cggcgtttgc acgtgaaatc gacccgcata ccaactatct ttccccgcgt aataccgaac
    11161 agttcaacac tgaaatgagt ttgtcgctgg aaggtattgg cgcagtgctg caaatggatg
    11221 atgactacac cgttatcaat tcgatggtgg caggaggtcc ggcagcgaag agcaaagcta
    11281 tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg gttgacgtga
    11341 ttggctggcg tcttgatgat gtggtggcct taattaaagg gcctaagggc agtaaagttc
    11401 gtctggaaat tttacctgct ggtaaaggga ccaagacgcg tactgtaacg ttgacccgtg
    11461 aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc ggtaaagaga
    11521 aagtcggcgt gctggatatt ccaggcttct atgtgggttt gacagacgat gtcaaagtgc
    11581 aactgcagaa actggaaaaa cagaatgtca gcagtgtgat tatcgacctg cgtagcaatg
    11641 gtggtggggc gttgactgaa gccgtatcgc tctccggtct gttcattcca tccggcccga
    11701 ttgttcaggt ccgcgataac aacggcaagg ttcgtgaaga cagcgacacc gacggacagg
    11761 tcttctataa aggcccgctg gtggtgctgg ttgaccgctt cagtgcttcg gcttcagaaa
    11821 tctttgccgc ggcaatgcag gattacggtc gtgccctggt tgtgggtgaa ccgacgtttg
    11881 gtaaaggcac cgttcagcag taccgttcat tgaaccgtat ttacgatcag atgttacgtc
    11941 ctgaatggcc agcgctgggt tctgtgcagt acacgatcca gaagttctat cgcgttaacg
    12001 gcggcagtac gcaacgtaaa ggcgtaacgc cagacatcat catgccgacg ggtaatgaag
    12061 aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc attgatgccg
    12121 cgacttatgt gaaatcagga gatttaacgg cctttgaacc ggagctgctg aaggaacata
    12181 atgcgcgtat cgcgaaagat cctgagttcc agaacatcat gaaggatatc gcgcgcttca
    12241 acgctatgaa ggacaagcgc aatatcgttt ctctgaatta cgctgtgcgt gagaaagaga
    12301 ataatgaaga tgatgcgacg cgtctggcgc gtttgaacga acgctttaaa cgcgaaggta
    12361 aaccggagtt gaagaaactg gatgatctac cgaaagatta ccaggagcca gatccttatc
    12421 tggatgagac ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc agacccgcgg
    12481 aacaacccgc tcccgtcaag taatatctat caggcacaag aaattgtgcc tgatttttta
    12541 acagcggcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
    12601 cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
    12661 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
    12721 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
    12781 gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt
    12841 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
    12901 accgcgtaac gaaagggaac gttggctggt caatactgta gcaacccagg ctcgtcaggc
    12961 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaatg cttttgcaac
    13021 tggtgcgcgc cgtgatgcct ctctggttgc tgtcagcact ggtttgctgc agaacatgag
    13081 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
    13141 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
    13201 tctggcgcag cttgccgcgg gttttatggg cggaaaccgt gatgaaggtg aagagagcaa
    13261 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
    13321 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccatgctg atgcgggttc
    13381 agcaaaactg gttggtcgcg agaaaatgat tgccgcgctg caacgcctga aaaccagcta
    13441 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacggtaagt cgaaatcgct
    13501 cagtgagttg ttcatgaccc acccgccgct ggataaacgt attgaagctc tgcgtacggg
    13561 tgaatacctg aagtaatatg acgtaaatac taaaaagcgc gtcctgatcg acgcgctttt
    13621 tttatgccct ggatcgtggc tgagtgatac gtaaaccact gacacaggca gcaatcactg
    13681 ccagaatagc cgcagctatc agcgagacgt gcgtaccatt atcaccaaac tgatttagca
    13741 tcagcgctac cagagccgcg ccgctactct gacccagaag acgagccgtc cctaacatgc
    13801 cactggctcc accgctacgt tcgcgaggag cggaggtaat aatggtgtgg ttattgggtg
    13861 actggaacaa gccaaatcca gcaccacata agatcatcgg ccagataata ttgatatccg
    13921 caggtgacgc tggcagcaga accagggaaa aaagccccgc agccatgatg aataacccta
    13981 aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccagt ggagccataa
    14041 ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
    14101 gcacggtttg caggtaaaag ggaagggaaa ccattgccag catttgtgcg cagaaagagc
    14161 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
    14221 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacgaca accattacca
    14281 ccagttccgc accaatcaat gtcagcgatt gcccctgagc gaaaccactc aatgcagtaa
    14341 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
    14401 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccca
    14461 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
    14521 ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctac
    14581 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
    14641 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
    14701 cgcagaacag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
    14761 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
    14821 cgttcactac ccagatggaa ctggccggcg tggcatgaag gtccgtagcg attgttggca
    14881 aggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta
    14941 atatcgcacc gtatcgcagg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
    15001 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
    15061 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccacccag gtgcgaaatt
    15121 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
    15181 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
    15241 caggcgctgg gtgaagagcg cgaaataggg ataaccgagt tgtcgcagcg cgtcatgatg
    15301 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
    15361 gaaggggagt cggagaaata ttcgctgacc ctgaaattgt ttgaactggg cgctcgcgcg
    15421 ttgcaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg
    15481 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta catccacaaa
    15541 attgactcaa tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
    15601 agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gatgcaaatt
    15661 cttgagggcg tggagtataa acgcagtacc gaacggacca tcatcagtac agaagcgtta
    15721 ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa
    15781 gaaggactgc gttgcattgc ggttccggta tttgatcgct ttggcgtggt cattgccggt
    15841 ctgagcatct ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca
    15901 atgttgcata ccgcggcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
    15961 tgatgagagt aagaacctgt ctgaatatca aacagacagg ttctttattt agcatgagaa
    16021 aaataaagtt gaaggtggcg ttatattaaa cgcacttgcg ataagaatat tttactcatg
    16081 agtgagaatc tggttattta ttacccttaa ccattatcga ccacaatatt gcttttgcgt
    16141 aacaacgggc aatctgttat ccccagaaaa ccacttttag tgtgcaggta ttgtaccgtg
    16201 ctggtgcctc tggcggtcag ataggtacat tgcaaaccta atcccgcggc attctctttg
    16261 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    16321 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    16381 tagcggcaat atgcgctgaa gcaagcgact cattctgaaa aagcacgaat atcgacatag
    16441 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aaacggagat
    16501 tggagtgaaa aagtttcgat gggtcgctct ggttgtcgtg gtgttggctt gcttgctgct
    16561 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    16621 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactggat tttcttcctc
    16681 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    16741 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    16801 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    16861 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    16921 aacgaacctg agaaagcaga taaaaagacc gttgagagtc aaaaatcggt tgaagacgaa
    16981 gatattattc gtctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    17041 tgaagggtta ctcaattcat gctgattata cgcactgtca tggtgcaact gcttcaaatt
    17101 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    17161 aaaaagatat ttaccccatt aatttattag gatgtttaca tcggatttgt gattaagtgt
    17221 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctaattca tcaatttttc
    17281 ttattttaaa tttacaatac ctttggagat tgacttctct ttggggta
//
LOCUS       DAJOTB010000100        17221 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJOTB010000100 DAJOTB010000000
VERSION     DAJOTB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.100"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(28..1482)
                     /gene="xylB"
                     /locus_tag="OHN28_003283"
     CDS             complement(28..1482)
                     /gene="xylB"
                     /locus_tag="OHN28_003283"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCQ2298621.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1553..2875)
                     /gene="xylA"
                     /locus_tag="OHN28_003284"
     CDS             complement(1553..2875)
                     /gene="xylA"
                     /locus_tag="OHN28_003284"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCQ2298622.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3241..4233
                     /gene="xylF"
                     /locus_tag="OHN28_003285"
     CDS             3241..4233
                     /gene="xylF"
                     /locus_tag="OHN28_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2298623.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDMVVKDGFHKESEL"
     gene            4311..5852
                     /gene="xylG"
                     /locus_tag="OHN28_003286"
     CDS             4311..5852
                     /gene="xylG"
                     /locus_tag="OHN28_003286"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298624.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5830..7011
                     /gene="xylH"
                     /locus_tag="OHN28_003287"
     CDS             5830..7011
                     /gene="xylH"
                     /locus_tag="OHN28_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCQ2298625.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7089..8267
                     /gene="xylR"
                     /locus_tag="OHN28_003288"
     CDS             7089..8267
                     /gene="xylR"
                     /locus_tag="OHN28_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCQ2298626.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8375..9199)
                     /locus_tag="OHN28_003289"
     CDS             complement(8375..9199)
                     /locus_tag="OHN28_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCQ2298627.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9519..11549
                     /gene="malS"
                     /locus_tag="OHN28_003290"
     CDS             9519..11549
                     /gene="malS"
                     /locus_tag="OHN28_003290"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2298628.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11727..12980
                     /gene="avtA"
                     /locus_tag="OHN28_003291"
     CDS             11727..12980
                     /gene="avtA"
                     /locus_tag="OHN28_003291"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCQ2298629.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13019..13492)
                     /locus_tag="OHN28_003292"
     CDS             complement(13019..13492)
                     /locus_tag="OHN28_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2298630.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13594..14442)
                     /gene="yiaJ"
                     /locus_tag="OHN28_003293"
     CDS             complement(13594..14442)
                     /gene="yiaJ"
                     /locus_tag="OHN28_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCQ2298631.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14643..15641
                     /gene="yiaK"
                     /locus_tag="OHN28_003294"
     CDS             14643..15641
                     /gene="yiaK"
                     /locus_tag="OHN28_003294"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCQ2298632.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15653..16117
                     /locus_tag="OHN28_003295"
     CDS             15653..16117
                     /locus_tag="OHN28_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2298633.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16140..17060
                     /locus_tag="OHN28_003296"
     CDS             16140..17060
                     /locus_tag="OHN28_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HCQ2298634.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT         4418 a         4346 c         4216 g         4241 t
ORIGIN      
        1 cccacccggt caggcagggg ataacgttta cgccattaat ggcagaagtt gctgatagag
       61 gcgacggaac gtttctcgtc gtggctgata agcggcataa cgctgcgcat ctggtagatg
      121 cgactgttct aacggtagtt gcggcaacaa ctcaatgagc gatttctctg gattcgccgc
      181 aatctgcgcc agccttgctg cgcccagtgc tggccccaca tcgccacccg tgcggtaatc
      241 cagttgctga ccgctaatgt ccgccagcat ttgacgccag tactcactac gcgccccgcc
      301 accaatcaac gtaacacttt gcggtttaat gccgcaggca tgcacgacat ccatgccatc
      361 tgccagcgca taacccacgc cttccagcac tgctcgcgcc agttcattgg ggccatgttg
      421 atgagtcaaa ccaaagaaaa cccctttcgc ctggggatta ttgtgtggtg tacgctcgcc
      481 agaaagataa ggcagaaacc aaactggctc ggcactttca tcagcctgtc gagctgcagt
      541 gattaaagca gggacattgc tcaggccggt taatttcgcg gcccaatcca gacacgaagc
      601 cgcactcagc attacagaca ttaaatgcca acgttgcggt aacgcatggc aaaagctatg
      661 tacggcgctt tctggcttgc ttaagaaccc ttcgctgaca gcaaaataga cccccgacgt
      721 ccccagcgat aacattgcct gattagcatc aaccattccc acaccaaccg caccagctgc
      781 attgtcgcca ccgcctgcga caactggcac cgccgccata ccccacgctt tcgcaacttc
      841 aggtagcaaa gcaccagtaa tttcgctgcc ttcgtataat gcgggcatct ggtcacgaga
      901 taagtggcaa gcctgcagca tgtcgtcact ccagtcacgc tttgcgacat ccagccacat
      961 ggtgccagct gcgtcagaca tatcgctggc aaactccccc gtcatacgca gacgcaagta
     1021 atctttcggt aataatactt tatcgatttg acggaatatc tccggctcat gccgctgaac
     1081 ccatagcaat ttaggcgcag taaatccggg catcatcagg ttgccggtaa tcacccgcga
     1141 ttgcggaact cgcgcttcca gcaaagtgca ctcttgcgca cagcgcccgt cgttccacaa
     1201 aatggcaggg cgtaacaccc gttgctgagc atccagtaag gttgcaccat gcatctgtcc
     1261 ggcaatacct aatgctttaa cgtcttgcag agaatgctga acgcccagag ctttcattgc
     1321 gcgatcagtt gcctgccacc actgttccgg gtcttgttcc gaccagagtg gatgcgggcg
     1381 cgaaacggtc agcttttccg tttgcgcagc aaccacctca ccctgctcgt tgagcaaaat
     1441 aacttttacg cccgaggtgc caagatctat cccgatatac atatcgatcg ttccttaaaa
     1501 aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttaac cgttatttgt
     1561 cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc tgatgcaccg
     1621 gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt tggcctttca
     1681 ggatttgctg gcctaattca ctattccagc cggaataacg ctgcgcgatg cgtttatcca
     1741 gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc caacgccatc gtatccatcg
     1801 cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt actttggcat
     1861 cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac atcaccagcg
     1921 cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc gcatcgccac
     1981 ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt tcatgatgga
     2041 aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc tctttttcca
     2101 gaccaaactg tttcaggaag ccatagaccg tcgcagcatc gtaatcatat tgatgtttgg
     2161 tcggttcttg cggtttcggt tcgataagca acgtgccctg gaaaccgatt ttatgtttat
     2221 gctcaaccac catctgcata aagcggccca gctgttcacg ctcctggcgc aagtcggtat
     2281 ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca ccgcccaatt
     2341 tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccaactgaag acttcaggat
     2401 ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta gcagttcccc
     2461 acagcagctt cacgccgctc tcttcttgct tgcctgccag gacatcaacc atttgcgcaa
     2521 aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca tcgtggaagc
     2581 aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct ttacgcttcg
     2641 ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc acaccaaaca
     2701 tatccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc aagtgctctt
     2761 ccatacgctt gcccaacacc agttcgtcgg gattgtagtg acggaatgct aacgggtttg
     2821 aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct tgcatattga
     2881 actccataat caggtaatgc cgcgggtgat ggatgatgtc gtaatattgg gcactccctt
     2941 tcagttgctc aattatgtta tttcacactg ctattgagat aattcacaag tgtgcgctcg
     3001 ctcgcaaaat aaaatgggat gatgaaactg ggtaattcct cgaagagaaa aatgcaataa
     3061 gtacaattgc gcaacaaaag taagatctcg gtcataaatc aagaaataaa ccaaaaatcg
     3121 taatcgaaag ataaaaatct gtaattgttt tcccctgttt agttgctaaa aattggttac
     3181 gtttatcgcg gtgattgtta cttattaaag ctgtcctcta actacagaag gccctacacc
     3241 atgaaaataa agaacattct actcaccctt tgcacctcac ttctgcttac caacgttgcg
     3301 gcacacgcca aagaagtcaa aataggtatg gcgattgatg atctccgtct tgaacgctgg
     3361 caaaaagatc gagatatttt tgtgaaaaaa gcagaatctc tcggcgcgaa agtatttgta
     3421 cagtctgcaa atggcaatga agaaacacaa atgtcgcaga ttgaaaacat gattaaccgg
     3481 ggtgtcgatg ttcttgtcat tattccttat aacggtcagg tattaagtaa cgttgtaaaa
     3541 gaagccaaac aagaaggtat taaagtatta gcttacgacc gtatgattaa cgatgcggat
     3601 atcgattttt atatttcttt cgataacgaa aaagtcggtg aactgcaggc aaaagccctg
     3661 gtcgatattg ttccgcaagg taattacttc ctgatgggcg gctcgccggt agataacaac
     3721 gccaagctgt tccgcgccgg gcaaatgaaa gtattaaaac cttatgttga ttccggaaaa
     3781 attaaagtcg ttggtgacca atgggttgat ggctggttac cggaaaacgc attgaaaatt
     3841 atggaaaacg cgctaaccgc caataataac aaaattgatg ctgtagttgc ctcaaacgat
     3901 gccaccgcag gaggggcaat tcaggcatta agcgcgcaag gtttatcagg gaaagtagca
     3961 atttccggcc aggatgcgga tcttgcaggt attaaacgta ttgctgccgg tacgcaaact
     4021 atgacggtgt ataaacctat tacattgttg gcaaatactg ccgcagaaat tgccgttgag
     4081 ttgggcaatg gtcaggagcc aaaagcggat accacactga ataatggcct gaaagatgtt
     4141 ccctcccgcc tgctgacacc gatcgatgtg aataaaaaca acatcaaaga tatggtagtt
     4201 aaagacggat tccacaaaga gagcgagctg taagcgttac gccccggcgc ggagcggggg
     4261 cgtgatttct ctccatgccg cgttaattaa ttggcttagg tggagtcgtt atgccttatc
     4321 tacttgaaat gaaaaatatt accaaaacct tcggcagtgt gaaggcgatt gataacgtca
     4381 gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg ggaaaatggg tcaggtaaat
     4441 caacgctgat gaaagtgctg tgtggtattt atccccatgg ttcctacgaa ggcgaaatta
     4501 tttttgcggg agaagagatt caggcgagtc acatccgcga taccgaacgc aaaggtatcg
     4561 ccattattca ccaggaattg gcgctggtga aagaactgac cgtgctggaa aatatcttcc
     4621 tgggtaacga aataacccat aatggcatta tggattatga cctgatgacg ctacgctgtc
     4681 agaagctgct cgcacaggtc agtttatcca tttcacctga tacccgcgtt ggcgatttag
     4741 gacttgggca acaacaactg gttgaaattg ccaaggcact taataaacag gtgcgtttgt
     4801 taattctcga tgagccgaca gcctcattaa ctgagcagga aacgtcggtt ttactggata
     4861 ttattcgcga tctacaacag cacggtatcg cctgtattta tatttcgcac aaactcaacg
     4921 aagtcaaagc gatttccgat acgatttgcg ttattcgcga cggtcaacac attggtacgc
     4981 gtgatgctgc tggaatgagt gaagacgata ttatcaccat gatggtcggg cgagagttaa
     5041 ccgcgcttta ccctaatgaa ccacatacca ccggagatga aatattacgt attgaacatc
     5101 tgacggcatg gcatccggtt aatcgtcata ttaaacgagt taatgatgtc tcgttttccc
     5161 tgaaacgtgg cgaaatactg ggtatcgccg gactcgttgg tgccggacgt accgagacta
     5221 ttcagtgcct gtttggtgtg tggcccggac aatgggaagg gaaaatttat attgatggca
     5281 aacaggtaga tattcgtaac tgtcagcaag ccatcgccca ggggattgcg atggtacccg
     5341 aagacagaaa gcgcgacggc atcgttccgg taatggcggt tggtaaaaat attaccctcg
     5401 ccgcactcaa aaaatttacc ggtagtatta gccagcttga tgacgcggca gagcaaaaat
     5461 gtattctgga atcaatccag caactcaaag ttaaaacgtc gtcccccgac cttgctattg
     5521 gacgtttgag cggcggcaat caacaaaaag cgatcctcgc tcgctgtctg ttacttaacc
     5581 cgcgcattct cattcttgat gaacccacca ggggtatcga tattggcgcg aaatacgaga
     5641 tctacaaatt aattaaccaa ctcgtccagc agggtattgc cgttattgtc atctcttccg
     5701 aattacctga agtgctcggc cttagcgatc gtgtactggt gatgcatgaa gggaaactaa
     5761 aagccaacct gataaatcat aacctgactc aggagcaggt gatggaagcc gcattgagga
     5821 gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat tagccgtacc gacatccggt
     5881 ggcttttccg ggctgaaatc actgaatttg caggtcttcg tgatgattgc agctatcatc
     5941 gcaatcatgc tgttctttac ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc
     6001 tccaacctgt tacgccaaac cgcaattacc ggcatcctcg cggtaggaat ggtgttcgtc
     6061 ataatttctg ctgaaatcga cctttccgtc ggctcaatga tggggctgtt aggtggcgtc
     6121 gcggcgattt gtgacgtctg gctaggctgg cctttgccac ttaccatcat tgtgacgttg
     6181 gttctgggac tgcttctcgg agcctggaac ggatggtggg tcgcgtaccg caaagtccct
     6241 tcatttattg tcaccctcgc gggcatgttg gcatttcgtg gcatactcat tggcatcacc
     6301 aacggcacga ctgtttcccc caccagcgcc gcgatgtcac aaattgggca aagctatcta
     6361 cctgccagca ccggcttcat cattggcgcg cttggcttaa tggcttttgt tggctggcaa
     6421 tggcgcggaa gaatgcgccg tcaggctttg ggtttgcagt ctccggcctc taccgcagta
     6481 gtcggtcgcc aggctttaac cgctatcatc gtattaggcg caatctggct gttgaatgat
     6541 taccgtggtg ttcccactcc tgttctgctg ctaacgttac tgttattggg cggcatgttt
     6601 atggcaacgc ggacggcatt tggacggcgc atttatgcca tcggcggcaa tctggaagcg
     6661 gcacgtctct ccgggattaa cgttgaacgc accaaacttg ccgtgttcgc tattaacgga
     6721 ttaatggtag ccatcgccgg attaatcctc agttctcgac tcggcgctgg ttcaccttct
     6781 gcgggaaata tcgccgaact ggacgcaatt gcagcatgtg tgattggcgg caccagtctg
     6841 gcaggcggcg tcgggagcgt ggccggagcg gtaatggggg cattcatcat ggcttcgctg
     6901 gataacggca tgagtatgat ggatgtaccg accttctggc agtatatcgt taaaggtgcg
     6961 attctgttgc tggcagtatg gatggactcc gcaaccaaac gccgttcttg attttgataa
     7021 aaattttctc aaagccggta acgtattacc ggttttgagt ttttgcatga ttcagcagga
     7081 aaagaaccat gtttactaaa cgtcaccgca tcacattact gttcaatgcc aataaagcct
     7141 atgaccggca ggtagtagaa ggcgtagggg aatatttaca ggcgtcacaa tcggaatggg
     7201 atattttcat tgaagaagat ttccgcgccc gcattgataa aatcaaggac tggttaggag
     7261 atggcgtcat tgccgacttc gacgacaaac aaatcgagca agcgctggct gatgtcgacg
     7321 tccccattgt tggggttggt ggctcgtatc accttgccga aagttaccca cccgttcatt
     7381 acattgccac cgataactat gcgctggttg aaagcgcatt tttgcattta aaagagaaag
     7441 gcgttaaccg ctttgctttt tatggtcttc cggaatcaag cggtaaacgt tgggccactg
     7501 aacgcgaata tgcatttcgt cagcttgtcg ctgaagaaaa gtatcgcgga gtggtttatc
     7561 aggggttaga aaccgcgcca gagaactggc aacacgcgca aaatcggctg gcagactggc
     7621 tacaaacgct accaccgcaa accgggatta ttgccgttac tgacgcccgg gcgcggcata
     7681 ttctgcaagt atgtgaacat ctacatattc ccgtaccgga aaaattatgc gtgattggca
     7741 tcgataacga agaactgacc cgctatctgt cgcgtgtcgc cctttcttcg gtcgctcagg
     7801 gcgcgcggca aatgggctat caggcggcaa aactgttgca tcgattatta gataaagaag
     7861 aaatgccgct acagcggatt ttggtcccac cagttcgcgt cattgaacgg cgctcaacag
     7921 attaccgctc gctgaccgat cccgccgtta ttcaggctat gcattacatt cgtaatcacg
     7981 cctgtaaagg gattaaagtg gatcaggtac tggatgcggt gggcatatca cgctccaatc
     8041 ttgagaagcg ttttaaagaa gaggtgggtg aaaccatcca tgccatgatt catgccgaaa
     8101 agctggagaa agcgcgcagt ctgctgattt caaccacctt gtcgatcaat gagatatcgc
     8161 aaatgtgcgg ttatccatcg ctgcaatatt tctactctgt ttttaaaaaa gcatatgaca
     8221 cgacgccaaa agagtatcgc gatgtaaata gcgaggtcat gttgtagcga tgatgagaat
     8281 tgtcggcgtc gcttcaggta actcagcaca accacctgat gggagcaaaa gccttatccg
     8341 actgacaagt cggataaggc tcgaggaaat gcaattacat atgcgccgcg attaaccgtt
     8401 ggttatcctg gtacattgca aacaggtagt tgttataact cttccctttg gtcgagtagc
     8461 ccttcagctt gtgaatcatc gctgtggcag tcacttcctg atccgcttta cgcagctgtg
     8521 cacgcgattt acggaacgaa gagtaagccg ggtgcgtatt gaggtttgta acataggcgc
     8581 tcaccgattc tttaacagaa ctaaactgtg agtacccttt cactttaccc ggcgcattgg
     8641 tacaacgtcc tttcatgcat ttcatgccga aaagattgtt gttgttgcgc gccagctttg
     8701 acgttcccca accgctttct gctgcagcca tcgtcgccac catactggta gggataatgt
     8761 ctacgcgttc aagcaaggta ttccacggga tttttcgcgt attaccagac cacttcacct
     8821 tgtagcgttt ggcgatgtct ttcagacgcg cacgctcagc aggtgaccat tggccctgat
     8881 actgttttga aatcagccag ttacgttccg cagtaatggc cgcattttgg ctggtaatgt
     8941 aaggcattac agtccggaga aacgcctttt tccttggtgt tccggaaggg tattttcgca
     9001 aatcaggaag tgaactactc tttgcactat tgcgagaata ctcttgttta ctgcttacct
     9061 gtttattact agctttagtt aagtgggact tttgactcgc tgttgttgtg tgcgtcttcg
     9121 ctaacacctc actcgaaaac accagagtga gtaacataag aatcatcgcc ccatatcgtc
     9181 gtatgggagt caaaatcatc aggtctcctg gtcggattta atcattccaa caccttatat
     9241 tttttacaaa tttgagagtt gaatctcaaa tcatatcaaa aatagctgtc aagagcaccc
     9301 caaggaatag tccaaatctg aaactatgtc acgagttaac gattcagatt ggcgctaaat
     9361 cgcagaaaat gtggggttta tcgcaaaatt cagccgtttt ttgcgcgcga tcgctcaccc
     9421 ttgcttctca tcctgtggag ttaccgctca gggatgagtt ttgtctggct tatcgctggc
     9481 aaactgtctg aaatcgcagc aataaggact catccgccat gaaactcgcc gcctgttttc
     9541 tgacactcct tcctggcttc gccgttgccg ccagctggac ttctccgggg tttcccgcct
     9601 ttagcgaaca ggggacggga acatttgtca gccacgcaca gttgcccaaa ggtacgcgtc
     9661 cactaacgct aaattttgac caacagtgct ggcagcctgc ggatgcgata aaactcaatc
     9721 agatgctttc cctgcaacct tgtagcaaca cgccgcctca atggcgattg ttcagggacg
     9781 gcgaatatac gctgcaacta gacacccgat ccggtacacc aacattgatg atttccatcc
     9841 agaacgccgt cgaaccggta gcaagcctgg tccgtgaatg cccgaaatgg gatggattac
     9901 cgctcacact ggatgtcagc gccacttttg cggagggagc cgcagtccgg gattattaca
     9961 gccagcaaat tgcgatagtg aagaacggtc aaataacgtt acaacccgct gccaccagca
    10021 acggtttact cctgctggaa cgggcagaaa ctgacacatc cgcccctttc gactggcata
    10081 acgccacggt ttactttgtg ctgacagatc gtttcgaaaa cggcgatccc agtaatgacc
    10141 agagttacgg acgtcataaa gacggtatgg cggaaattgg cacttttcac ggcggcgatt
    10201 tacgcggcct gaccaacaaa ctggattacc tccagcaact gggcgtcaat gctttatgga
    10261 taagtgcccc atttgagcaa attcacggct gggtcggcgg cggtacaaaa ggtgatttcc
    10321 cgcattatgc ctatcatggt tattacacac aggactggac gaatcttgat gccaatatgg
    10381 gcagtgaagc cgatctacgg acgctggttg atagcgcgca tcagcgcggt attcgtattc
    10441 tctttgatgt cgtgatgaac cacactggct atgccacgct ggcggatatg caggagtatc
    10501 agtttggcgc gttatatctt tctggtgacg aagtgaaaaa aacgctgggt gaacgctgga
    10561 acgactggaa acctgccgcc gggcaaacct ggcatagctt taacgattac attaatttca
    10621 gcgacaaaac aggctgggat aaatggtggg gaaaaaactg gatcagaacg gatatcggcg
    10681 attacgacaa tcctggattc gacgatctca ctatgtcgct ggcctttttg ccggatatca
    10741 aaaccgaatc aactaccgct tctggtctgc cagtgttcta taaaaacaaa acggataccc
    10801 acgctaaaat catcgacggc tttacccctc gcgattactt aacccactgg ttaagtcagt
    10861 gggtccgcga ctatgggatt gatggttttc gggtcgatac cgccaaacat gttgagttgc
    10921 ccgcctggca gcaactgaaa accgaagcca gcgctgcact tcacgaatgg aaaaaagcta
    10981 accccgacaa agcattagat gacaaacctt tctggatgac cggtgaagcc tggggccacg
    11041 gcgtgatgca aagtgactac tatcgccacg gcttcgatgc gatgatcaat ttcgattatc
    11101 aggagcaggc ggcgaaagct gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa
    11161 tggcggagaa attgcaggat ttcaacgtgt tgagctacct ctcgtcgcat gatacccgcc
    11221 tgttccgtga agggggcgac aaagcagcag agttattatt attagcacca ggcgcggtac
    11281 aaatttttta tggcgatgaa tcctcgcgtc cgttcggtcc taccggttct gatccgctgc
    11341 aaggtacgcg ttcggatatg aactggcagg atgttagcgg taaatctgcc gccagcgtcg
    11401 cgcactggca gaaaatcagc cagttccgcg cccgccatcc cgcaattggc gcgggcaaac
    11461 aaacgacact ttcgctgaag cagggctacg gctttgttcg tgagcatggc gacgataaag
    11521 tgctggtcat ctgggcgggg caacagtgac ttttccggct tcccgttcgt gagtacctcg
    11581 ggaagccgcc aaccaggata aaatgtcagc cctaatcagc attgcaggat aaagccccgc
    11641 tcactcttca acagaccgat ttgcacccca gcaaatgtag cgttattgtt accttccttg
    11701 ctacagagtt cgacagatat cccgctatga cattctccct ttttggtgac aaatttaccc
    11761 gccactccgg cattacgctg ctgatggaag atctgaacga cggtttacgc acgcctggcg
    11821 cgattatgct cggcggcggt aatccggcgc agatcccgga aatgcaggac tacttccaga
    11881 cgctactgac cgacatgctg gaaagtggca aagcgactga tgcactgtgc aattacgacg
    11941 gtccacaggg gaaaacggag ctactcacac tgcttgccgg aatgctgcgc gagaagttgg
    12001 gctgggatat cgaaccacag aatattgcac taacaaacgg cagccagagc gcgtttttct
    12061 acttatttaa tctgtttgcc ggacgccgtg ccgatggtcg ggtcaaaaaa gtgctgttcc
    12121 cgctggcacc ggaatacatt ggctacgctg acgccggact ggaagaagat ctgtttgtct
    12181 ctgcgcgtcc gaatattgaa ctgctgccgg aaggccagtt taaataccac gtcgattttg
    12241 agcatctaca tattggcgaa gaaaccggga tgatttgcgt ctcccggccg acgaatccaa
    12301 caggcaatgt gattactgac gaagagttgc tgaagcttga cgcgctggcg aatcaacacg
    12361 gcattccgct ggtgattgat aacgcttatg gcgtcccgtt cccgggtatc atcttcagcg
    12421 aagcgcgccc gctatggaat ccgaatatcg tgctgtgcat gagtctttcc aagctggggc
    12481 tacctggctc ccgctgcggc attatcatcg ccaatgaaaa aatcatcacc gccatcacca
    12541 atatgaacgg cattatcagc ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa
    12601 tgattaagcg taacgatctg ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc
    12661 agcgtgttca ggaaactatc gccatcattc gccgctattt accggaagat cgctgcctga
    12721 ttcataaacc ggaaggagcc attttcctct ggctatggtt taaggatttg cccattacga
    12781 ccgagcagct ctaccagcgc ctgaaagcac gcggcgtgct gatggtgccg gggcacaact
    12841 tcttcccggg actggataaa ccgtggccgc atacgcatca atgtatgcgc atgaactacg
    12901 taccagagcc ggagaaaatt gaggcgggtg tgaagattct ggcggaagag atagaaagag
    12961 cctgggctga aagccattaa tacgctattc cggcgttgag aaacgccgga aaaacgtttc
    13021 aaacaggctg ctgccgtagc cgatgccgct gcactttctc gacatccatg cactgcaacg
    13081 cctttgtcgg gcaggcttca acacaggccg gccccgtcgc ccgatgccag cacagatcgc
    13141 acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag
    13201 ccagcataca gcttttacag ccaatgcaac gtgattgttc aacgaaaata tgcccatgtt
    13261 cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac
    13321 aggctactgc cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt
    13381 ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac
    13441 aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcactc
    13501 ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga tccaacgaag
    13561 gaaatgttaa agttttggca cttgtccaaa gggttatgta atcgcgccct ggtcgtccct
    13621 gacagtaaat cccagctcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag
    13681 atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg
    13741 cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc
    13801 ttctctgtcc atcgccgctc cactttcacg aatgtgcgcc agttcgtcga acatcgcggg
    13861 cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggc tttcccagta
    13921 tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta
    13981 gagcggcata tgctggccta tataggcacg ggttcgcagc atcccggttg tgggttccag
    14041 cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt
    14101 ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca gcgaagatag
    14161 cgccttctgc ccgacggcaa taaatttggt ggtcaggcga taactccctg cggcgggcgc
    14221 ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt
    14281 taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat
    14341 ctcaatcagc atcaacccac gaaacagact ctggcttccg gctggacgct ctttttcctg
    14401 cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg
    14461 tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca cttgattttt
    14521 aagttttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat atatttgaaa
    14581 tcaagtttcg tatattgaaa ttttaagcca aaaaagcgat caaaaaaaca aggaagcctg
    14641 ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta atttcacgcg
    14701 gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc accgaatccg
    14761 gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg
    14821 atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg
    14881 acgcccagcg ttcgatcggt aacctgacag cgaaaaagat gatggatcgc gccattgaac
    14941 tggctgccga tcacggtatt ggtctggtgg cactacgtaa tgccaaccac tggatgcgcg
    15001 gcggcagcta cggctggcag gcggcggaaa aaggctacat tggcatttgc tggaccaact
    15061 ccatcgccgt aatgccaccg tggggcgcaa aagagtgtcg catcggcacc aacccgctga
    15121 tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg atgttctctt
    15181 acggcatgtt agaagttaat cgcctggctg gtcgtcagct cccggtagat ggtggctttg
    15241 atgatgaggg caatttgacc aaagaacctg gcgttatcga gaaaaatcgc cgcattttgc
    15301 cgatgggcta ctggaaaggt tcgggcatgt cgattgtgct ggatatgatc gctaccctcc
    15361 tttctgacgg cgcatcggtt gccgaagtca ccgaggacaa cagcgacgaa tacggcattt
    15421 cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc cgcgatgcca
    15481 aactgcaacg cattatggat tacgttacca gtgccgagcg tgctgacgaa aatcaggcca
    15541 ttcgcttacc cggccacgaa tttactaccc tgctggcaga aaaccgccgt aacggtatta
    15601 ccgttgatga cagcgtgtgg gcaaaaatcc aggcgttata aggagataag ccatgatttt
    15661 tggacatatc gcgcagccta atccgtgccg tttgcccgcc gccattgaaa aggcgctcga
    15721 ttttctgcgc accaccgact tcaacgccct ggatccgggc gttgtcgaaa tcgacggcaa
    15781 gaatatttat gcgcaaatta tcgatttaac cacacgcgaa gcggtggaaa accgtccgga
    15841 agtgcatcgt cggtatatcg atattcagtt tctggcgtgg ggtgaagaga aaattggcat
    15901 tgccattgat acgggaaaca ataaagtcag cgaatcacta ttagagcagc gcgatattat
    15961 tttttatcac gatagtgaac atgaatcatt tatagaaatg ataccgggta gttacgctat
    16021 tttcttcccg caggatgtac atcgcccggc atgtaataaa aatgccacta cggcaatacg
    16081 caagatcgtg gttaaagtgg ccctcgctgc tttataaaaa atgattactg gagcacataa
    16141 tgaaaacgaa taatgccggt tatattatcg gcgcgtatcc ctgtgcacct tcttttcacc
    16201 aaagaagtga agaggaagag aaggatttct ggcgacaatt atctgagact ccagacattt
    16261 tgggtctgga acaaccttgc ctggaaaatt ttcaccctct tggcgatcaa tggttactcc
    16321 gtcatacgcc agagagctgg aaatttgtcg tcaccgcagt gatggaaacc atgcgtcgcc
    16381 gcagcgaaaa cagcgggttt ggtctcgcct ccagtgatga agaacagcgc caggcctgcg
    16441 tggcgtacta tcgccacctg ttcaataaaa tcaacgcatt gcaggctaat aaagtgctcg
    16501 cactggcgct ccaggctgca ccgttagcaa cgaatcctaa tgttatgcaa gcgacggacg
    16561 cttttgcccg ctcattgaaa gaaatcgcca gttgggactg gccgtgcaag ctggtgctgg
    16621 aacactgcga cgccatgaca tcttcttccc cccgaaaagg ctttttgcct ttagaaaatg
    16681 tgctggaagt tattaccgat tatgacatca gcatctgtat caactgggcg cgctctgcca
    16741 ttgaagggcg caatacaacg ctgccgctga cccacaccca aatggcgaaa caagccggaa
    16801 aactcggcgc gttgatgttc tctggtacga cgctaaacgg ggcatacggc gaatggcagg
    16861 atttacacgc accgtttgca ccattctgcg cagaaagcct gatgaccaca gatcatgttc
    16921 gcgagttatt taatgtagca gaatcgtcaa cattgcattt tgctggcatc aagctactgg
    16981 aaataaatgc gacggctgat gtgcaccatc gcatcgaaat attacgtaac ggcattcact
    17041 ctctgaatga atctcgttaa atagatccgc ttatatatca ctttgaatat ctaattagcc
    17101 gttttctgat aattattacg aatattaaca acattagcgc ataaaaaata ttgcataacc
    17161 agaaggactc gttatgaaac ggatactcga aggcattctt gcgatcatca tcgcaatact
    17221 g
//
LOCUS       DAJOTB010000101        17172 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJOTB010000101 DAJOTB010000000
VERSION     DAJOTB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.101"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..463
                     /locus_tag="OHN28_003297"
     CDS             <1..463
                     /locus_tag="OHN28_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2298635.1"
                     /translation="AGCQSNADDHAADVYQTDQLNTKQETKTVNIISILPAKVAVDNA
                     QNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAAAGSMVNDKTLVE
                     GVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            479..1018
                     /locus_tag="OHN28_003298"
     CDS             479..1018
                     /locus_tag="OHN28_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCQ2298636.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1111..2688)
                     /gene="guaA"
                     /locus_tag="OHN28_003299"
     CDS             complement(1111..2688)
                     /gene="guaA"
                     /locus_tag="OHN28_003299"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCQ2298637.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2757..4223)
                     /gene="guaB"
                     /locus_tag="OHN28_003300"
     CDS             complement(2757..4223)
                     /gene="guaB"
                     /locus_tag="OHN28_003300"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCQ2298638.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4385..5752
                     /gene="xseA"
                     /locus_tag="OHN28_003301"
     CDS             4385..5752
                     /gene="xseA"
                     /locus_tag="OHN28_003301"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCQ2298639.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGATLHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(5809..13890)
                     /locus_tag="OHN28_003302"
     CDS             complement(5809..13890)
                     /locus_tag="OHN28_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HCQ2298640.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(14000..14992)
                     /locus_tag="OHN28_003303"
     CDS             complement(14000..14992)
                     /locus_tag="OHN28_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298641.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            complement(15048..>17172)
                     /gene="sinH"
                     /locus_tag="OHN28_003304"
     CDS             complement(15048..>17172)
                     /gene="sinH"
                     /locus_tag="OHN28_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HCQ2298642.1"
                     /translation="FAAPEINVKQNESLPDLGSQAAQQDEQTNKGKSLKERGADYVIN
                     SATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPYGKVRSNLSIGQGGDLDG
                     SSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHNFDKWLLGGNIFYDYDFT
                     RGHRRLGIGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEERPASGWDIRAEAWLPAY
                     PQLGGKIVFEHYYGDEVALFGTDNLEKDPFAVTLGVKYQPVPLIAVGTDFKAGTGDNT
                     DTTVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVERNNFIVLEYKEKDPLDVT
                     LWLKADAMNEHPECVIKDTPEEAIGLEKCKWTINALVNHHYKIVAVSWQAKNNAARTL
                     VMPVIKENTLTEGNNNHWNLVLPVWQYSSDKAEQGKLNTWRVRLALKDEKGNRQNSGV
                     VEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGVVMDLDITDSFGDNTDRN
                     GNVLPQDNLNPQLFDANDKKVTLTNNPCTTETPCVFIAKQDKEKGTVTLSSTLPGTFR
                     WKAKAAPYDDSNYVDVTFLGADIGGLNAFIYRVGAAKSVNLIGNKEPLPLNSRYRFVL
                     WRDANKDGVFQLSEKLTDEEMKQYDYQWEFTGHSVNGNTGAQANTTNVDIEIPATNKE
                     AAAKFSAQVTDGVQGYGLQVNYSKT"
BASE COUNT         3876 a         4699 c         4369 g         4228 t
ORIGIN      
        1 ggcaggatgc cagtcaaatg ctgatgatca tgccgccgat gtttatcaaa ccgatcaact
       61 gaataccaaa caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc
      121 cgtagacaac gcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgctgt
      181 cgctggcggt gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg
      241 tgcagttggc ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt
      301 agtggaaggt gtttctttaa catataagga aggcaccaaa gtgtatacct ccacccaagt
      361 gggtaaagag tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga
      421 aacgcgtatt cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat
      481 gaagaaagtt tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc
      541 attgcaggat caactctcgg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca
      601 aaggcagcat gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg
      661 tcgcgcaaac gcccaggccg cggctaataa aagagcggca acggcagcag cgaataagaa
      721 agctcgtcag gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta
      781 tgaagatgag ctacgtagct tagagattca gaaacaaaaa ctggcgctgg cgaaagaaga
      841 agcccgcgtt aagcgcgaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca
      901 aaccgatgtg gtgcaatctg aagctgacgc aaacagaaat atgactgaag gcggtcgcga
      961 tctgatgaaa agcgtgggta aagcagaaga gaataaatca gatagctggt ttaactaaac
     1021 gatgttagta acttcaaacc tataattctt taggataaaa aaccctctgt agtaacagag
     1081 ggttttgttc attcatagtg cagggtcaaa tcattcccac tcaatagttg ctggtggctt
     1141 gccgctgatg tcatacacca cgcgggaaat accattcact tcattgataa tgcggttgga
     1201 aactcgaccg aggaaatcgt acggcagatg tgcccagtgt gcggtcataa agtcgatggt
     1261 ttcgacagca cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac
     1321 ggaacgtact ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca ggtccgcttt
     1381 acgcagttct tcaatgaaga tggcgtcagc acggcgcagc aggtcacaat actctttctt
     1441 cacttccccc agaacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacaacat
     1501 gtcatacggc aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt
     1561 cagcggttca accaggccca tcttcatctc tttcggcagc ccacccacgt tgtggtgaga
     1621 tttgatgacg tgtgctttac cggttgcaga agcagcagat tcgataacgt cagggtagat
     1681 ggtgccctgc gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc
     1741 aacgaaaacg cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc
     1801 tgacaagaag cgatcttctg ccggtacgtg aacaatgttc agaccaaagt gatcgccaaa
     1861 catatccaga acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca
     1921 agtcaggttt ttaccgatag cgcggtgcaa cagcattgcg gttacggagg aatccacgcc
     1981 accagagagg ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gggctacagc
     2041 gtcgtcgata attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa
     2101 acgctccagc atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc
     2161 atagaagcgt ttttcttcgt tggccatgat ggcaaacggg cagctttcgg tgctggctac
     2221 ggtgacgaag tcggacggga tagcggtaac tttatcgccg tggctcatcc agacgtccag
     2281 cagcggttta ccatctgcgg tcagagcatc ttcgataccg cgaaccagtg cgctgtcgtt
     2341 tacgacttca acctgcgcgt agccaaattc acgttcatta gaagcctcaa cgtgaccgcc
     2401 caactgcatc gccatggtct gcataccata gcaaacgccg aataccggta cgcctgcttc
     2461 aaagacatac tgcggcgcac gcgggctgtt ttcttcagta gtactttccg ggccgccgga
     2521 aagaataatg ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca
     2581 cagttcgcag taaaccccca gttcacgcac gcggcgcgca accagttgag tatactggga
     2641 accgaagtcc agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc
     2701 cgaggcaagt gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag
     2761 gagcccagac ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc
     2821 tgaatacccg caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta
     2881 ccacagccgg tcaggcccat acaggagcgc aggccgccca tctgctggtg aatgatctct
     2941 ttcaggcgac ctttataggc tacgcgacct tcgatacctt ccggcaccag tttgtcggca
     3001 gcgttgtcgc tctggaagta acggtcagaa gaacctttgg acatcgcgcc cagggaaccc
     3061 ataccacggt aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct
     3121 tcagtacctg ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagcttta
     3181 gcgatatcgc ctgagaaacg gataccgccg tcagcgataa ccggaatgcc ggtaccttcc
     3241 agggcttcta ctgcatcagc aacggcggtg atctgcggaa caccgacacc agtcacgata
     3301 cgcgtcgtac agatagaacc agggccgata cccactttaa cggcactgca accggcttct
     3361 gccagcgcgc gggcacctgc agcagttgcc acgttgccgc cgatgatttg caggtcagga
     3421 tatttagcgc gagtttcacg gatacgctgc agaacacctt cagagtgacc gtgagaggag
     3481 tcgatcagca gaacgtcaac gcctgcggca accagcgcat caacacgctc ttcgttacca
     3541 gcgcccgcgc caaccgctgc acctacacgc agacggcctt gttcgtcttt acacgcgttc
     3601 ggtttacgtt ccgctttctg gaagtcctta acagtgatca tgccgatcag gtggaattca
     3661 tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca
     3721 cgggcttcgc cttcacgcac ggttaccaga cgctctttcg gcgtcatgta aacgctaacc
     3781 ggctggctca agtcggtcac aaaacgcacg tcacgaccgg tgataatgcc gaccagttcg
     3841 ttttcttcgg taacaaccgg gtagccagca aagccgttac gctcggtcag ttctttcact
     3901 tcacgcagcg tcgtagttgg cagtacggtc tgcggatcgg ttaccacgcc agattcgtgt
     3961 tttttcacac ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag
     4021 ccgataccgc cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt
     4081 gctgcggaaa gcatagggat attcagacga atagttttcg tcagctgagt gctgaggtca
     4141 gcagtatttg gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga
     4201 gcttctttag cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc
     4261 cgcggcatta tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct
     4321 atccccggag ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc
     4381 tcacatgtta ccttctcaat cccctgcaat ttttaccgtt agccgcctga atcaaacggt
     4441 tcgtctgctg cttgagcatg agatggggca ggtgtggatc agcggcgaaa tctctaattt
     4501 tacacaacca gcctccggtc actggtactt tacgcttaaa gacgataccg cgcaggtgcg
     4561 ctgtgcgatg ttccgcaaca gcaaccgccg ggtcaccttc cacccacagc acgggcaaca
     4621 ggttttagtt cgcgccaata ttacactcta cgagccgcgc ggcgattatc aaattatcgt
     4681 cgaaagtatg cagccggccg gtgagggatt gctgcaacag aagtacgaac agcttaaagc
     4741 gaagttgcag gctgaaggtt tgttcgatct gcaatacaaa aaatcactcc cctcccctgc
     4801 gcattgcgtt ggtgtgatca cctcaaaaac cggtgctaca ctacatgata ttttgcatgt
     4861 gttaaaacgt cgcgatcctt ctctgccggt gatcatctac cccaccgccg tacaggggga
     4921 tgatgcgccg gggcaaatcg ttcgtgccat tgaactggcg aataagcgca acgagtgtga
     4981 cgtgttgatc gttggccgtg gcggcggttc gctggaagat ttatggagct ttaacgacga
     5041 acgcgtggcg cgggcgattt ttgccagtcg cattccgata gtcagcgccg tcgggcatga
     5101 aaccgatgtg acgattgccg actttgttgc tgatctgcgt gcgccaacgc cgtcagccgc
     5161 cgctgaagtg gtgagccgaa atcagcaaga attgctacgt caggtgcaat ctgcccgtca
     5221 acggctggaa atggcgatgg actattatct cgccaatcgc acgcggcgct ttacgcagat
     5281 ccatcaccgc ttgcagcaac agcatccgca gctccggctg gcacgccagc aaactatgct
     5341 cgaacgtctg caaaagcgga tgagctttgc gctggagaat cagcttaaac gcgcgggtca
     5401 gcaacagcag cgattaacac ggcaactcgt ccaccaaaat ccgcagtcgc gtattcatcg
     5461 cgcgcaaacg cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgcacagct
     5521 tagcgccacg cgcgaacggt ttggtaacgc ggtaacacac ctcgaagcgg ttagcccgct
     5581 gtcaacgctg gcgcgcggtt atagcgtcac cagcgccgcc gatggcgcag tgttgaagca
     5641 ggtgaagcag gtgaaagtgg gcgatacgtt gaccacgcga ctgggagatg gcgtggtcgt
     5701 cagtgaagtg agtgcggtga cgaaaacccg caagccgcgt aaaaaaacct gatagcccgt
     5761 tgcgtatcag gctttcggaa cctcatattg atagggtggt gggcaaggtt attggaaaca
     5821 ggttgcaaac cccggcgttt tcgcaggctg aatgccacag ttgagactgc cagagccttc
     5881 ctcaacacca caatagaacg atccagcctt atcagtagat aaatacggga agcttatcga
     5941 tgacggccat cccaatgtgc taaagccgga gtgtaatgtt tgtaattcgg cactgctcgg
     6001 cacgttattc atactaccgc actgccgtgc gacatcccat ttagtggaat ctataacatg
     6061 tgccagcgcc caggtttcat tattcacatt gggcgttgct gctgcggtaa cccctgacgg
     6121 caactccgat tttaatagcg ggcgatgcag catcttgcca cttaccgtgg cggtatccgc
     6181 catatgaccc cagaaatacg ccgtggcgac attaggactg gtcggcacgg tgaaaatcgt
     6241 gtccagcgtc gccgttattg tggcgttatc cgtcagcgtc accgtcaacg gcgttttata
     6301 tcctggcgta ttatcctgac caacggtaaa acttgctgta ccgtctgctc cagtaactcc
     6361 atcgattgta ttcccgctat cagccaacgt gactgccgca ccggatgagg gttgtacagt
     6421 caaatcgtcc attgtatcca catcgccata caacgtcgcc ccgctattac gcggcgtagc
     6481 gtcaccacgt ttgaggataa acgaaatatt cggcactggt ttcccgacac tgtctttcac
     6541 ggtaacgaca acgggaacgg cgtcgccttt tttcaccttc gccgcctgcg tactgctatc
     6601 cagtgccgtc gatgtcaacg tgacgctgga gacagtcgca cgcgggttaa ccaggcaccc
     6661 ttgatacgcg gtattcaccg tcccccacgc tgaaccatta ttcagaccga ctacctgcgg
     6721 attactgcct cccagccagt tatccagcgc ccaccatgta tactgcgtag accaaccgat
     6781 ttgtgtcgtc agcgtgttcc ccggatagcg agcatagagc gattctaaat catcactcaa
     6841 tggccgacgt gccgcttcac aagggctgag attgctgtca gccctctgcg ttgcgttaaa
     6901 cacgggccag acttcattgt tactattgac cttgctaacc cctgatgtcg acggcagctc
     6961 tttccacaat ttaggtcttt caaagaccac accgttttct gccgttaccg tatccggcat
     7021 atgaccccag taggccgcca ttgcggaatc agggctggtg acaacggtga agatcgcatc
     7081 aaggacagtg gtggtttccg tatcacgtgc caggatggcg gtaaacggtg ttgccaggcc
     7141 gacagtatca ggctgttcaa cattgaatat cgccgtacca tcgctgccgg taaaaccaag
     7201 ccattgcgtg ccgtgtgagg cgagagtgaa tgttgccgac acgggtgcca gttccgtcaa
     7261 taccatcaca tttgctgccg tgtttacaat ggttcctgag cggctcatcg cattatcacg
     7321 cctgagcgtt atcgcttcgc ccgccagcgg gttgccatca cgatctttga ccataaccac
     7381 aattggcaat gggttatctt ttttcactga cgcgctaggt cggccgccat tgctgagggt
     7441 tctggcgtca tccagtgcgg atgacgtcaa tgttacgcta acgcctgccg gatgcggctg
     7501 cgccagacag agcatcagtg cgttggttgc agaattcttc gcgatagctc ctgttctcag
     7561 gctgacagtc tgataagacc acgttgttgt acccagtatg gccatatcat aagcccacca
     7621 gttacccgca ttcatcggta acccaaggtc agtcattatt gcgccgttcg gatgatcact
     7681 atacaaatcc aacagttcct tactgcgcgg ttgattctcg agtgggcaat ctgtagatgt
     7741 taaattttgc aagttgctca tatacgacca aacttcgccg ttagaactaa tcgataagcc
     7801 tgagctggtt tcggttttca gcaggggtcg tttgaaggtg acgcccgccg aactggtaaa
     7861 ggtgtccggc atatgccccc agaatttagc actcggtgaa tctgggctgg taatcaccgt
     7921 gtagataaca tccagcgtgg cgactttcgt tgtatcacgc gccagcgtca cggttaacgg
     7981 cgtcgccaac ccggtagtag agtactgccc aacctcaaag gtggctgtgc catcactgcc
     8041 cgtcgttgcg agagtttttg ctccacttgc tgtcaacacg ctatccctac tcaccggaac
     8101 caatgccgtt acggtaagat cgtctgcact cgtgttaacg gttgctttcg cgcgatccag
     8161 cgtcgtttca cgcgaaaggg taaagtcggc atatggcatc gggttcccgg cgctatcacg
     8221 cacggtcacc gtcaacggaa tcagctcacc tttcttcgct acagcgctcg gtcgtccatc
     8281 attgctgagc gattttgctg catcctgcgc cgttgaggta agctctattg tgactgcttc
     8341 cgtatgaggc tctaccaaac acatcactat cgccgtatag ctggacgatg cctgaatcaa
     8401 ctgaccattt cccggaacaa acgcctggtt actccagcta ccgcccgcta ccgccatcat
     8461 gtcataatcc caccagttcc ctgcagcagt cggcaagccg agcaccgttg ccagttgacc
     8521 attgggataa tcgacataca gcccctgcat atcagtcgtc agcggttgat acgcctcatc
     8581 acacccagcc ttgctgacgt ccgctttctc agtcgcggtc aggagcgacc agacttcatt
     8641 attggccgtt ttcgccgtac cgccagagag ttcggctttg aggaaaggcc gtttgaaggt
     8701 gacgccttcg ctgctggtaa aggtttccgc catatggccc caattggtcg ccagcggcgt
     8761 atccgggctg gtggtgacgg taaagatcac cgacaaggtg tccgacgaca cgccagtatc
     8821 gtttaataga gcataaaagt cggttcttaa cccagtggaa gcatcctgtc ggagcgtcat
     8881 cgtagtggtt ccatctggcc ctgttacacc atacaggatg gtcgttgcac tggtatccac
     8941 cgtcgcaccc gccgcagtcg tgactgacac tttctgcgat gacgtcacat tctgacgatt
     9001 gatcgagttc gcgtgtttca gggcaaacgg cgtattgccg actaaattcc cctgcgcatc
     9061 tttagtcgaa atgcggatcg acacctcatc ccccttcttc gctttgacgg cgctaagtgt
     9121 gctgtcaatg ctgacaaact gcccggggtc tgaggcttcc agcgtaatct ggctggcagt
     9181 catcaacggc gttgtacggc aggtttgtaa cgccagcacg gtggatacga tttgcgaaac
     9241 tgcgccagaa tttagttgca ccacattgta gtaacggctc cctgtttgtg tattactcgg
     9301 ggtattggtg agataactca tatttaccgg ccatccctgt accgtattca tcgcattacc
     9361 tctattggcg tcatacaaag acttcagacc atccagcgtt ggcatataat ctgtaccaca
     9421 cccattcatg ctggtgtcag ccgactgagt cgaggtgaat cttgcccaac tttcaccgtt
     9481 ctcaaacgtg ctatatcgtg catagttgtt ggtaagctcg ccaaacagca acggtcgctt
     9541 ataaaccgaa ccatcggagg ccgtcaccgt ttcggccata tgtccccaaa aattcgcttt
     9601 tggcgaatcc gggctggtca cgacggtaaa gaccaccggt agcatcgcct tgacgctgct
     9661 ggacgtatcc agttttgccg tgagatcggt tcttaacccg gtactgttat cctgttttag
     9721 ggtaaatgtc gttgacccgt cggcaccggt cacgccgtaa atggtttcac tcgttgactg
     9781 gaaattattt cgcgtatttc cccacgcatc cgtcaccgtc agcgcgccca cactcatcgt
     9841 cgccgatgaa ttagcccgac ttacactgtt ggctcgcgtc aggataaatg ccgtattgcc
     9901 cacaaggttg ccttgcgcat ctttggtgat gacgcgcacc acggattctt cgcttttttt
     9961 cgcttttacc acgttatagg tggtatccag cgtcaccgga tctgccgctt ccagcaggat
    10021 ttggctcacc gcggctatcg gcgcggtctg gcaggtcagc agctccagcg tcgttgatga
    10081 cactgccgca ctcgtgccat tgctgagatc taccgccttg tagttacgct gctccatgct
    10141 gccgttgtcg gaagtgttgg ataaatagcg cgttgccacc ggccagccct gcacggtttt
    10201 catggtgttg cccgcattat tagcaaacag tgacgtcaga ccggcctgag tcggaatata
    10261 gttggttcca cagccgctgt acgtcgtgga aaaacttgca taattaatgt taaacaacgc
    10321 ccagttttca ttgtcttcag acgttgatgt tcgcccagtc tgataggcca gttccttcaa
    10381 caacagcggg cgcttaaata ccgtaccgtc cgcagcagtc accgtttccg gcatatgtcc
    10441 ccacattttt gccaggcggc tatccgggct ggtcaccacc gtaaagattg tgtcgatgct
    10501 ggattttttc gtcgcatcag agtaaagcat tgccgtcagc gccgttttgg tgccgtgagt
    10561 atccgggcgg gtaatattga gaattttgct gccgtcattg tcggtcatgg cggtatacac
    10621 cgttgcagtg tcgttcaacg tggtttcatc tgccagcccg ccattgacta ccaccggcgc
    10681 aaccagcgca tcgccgctgc ccgccacatg tttttcgccg ctacgggtat agccatcacc
    10741 gcgattgagg gtaaatggca tatccgctaa cgcgttgccc tgtgcgtctt ttaccgtcac
    10801 tttaacctgt agcgtctcgc cttttttcag cgttgccgcg ctattcccgg tattccattg
    10861 cgctttatca accacctcca gcgtaatgct gctggcaggt ttgttcgccg tcttcaggca
    10921 actgacgtaa accggcgtat cgccgccaat agtttgtgcg ccatcgttca gcgctatcgt
    10981 gaaaaagtgt ggagttttat ctgccggtga gttgctccag taaggctgac gctgtgttgg
    11041 ccaaccgtgc gccgtttgca ccgcgttacc actgttggcg ttatacagcg cacttagctg
    11101 ggaacgacgt ggcaacatat tggtgtcgca gccaccttta ctggtactgg acgtattggc
    11161 ctggctcacg cgcgcccaga tttcgttatt ttcggtcagg gttccgtccg ggctggcgac
    11221 ttccgacgcc agtttaggcc gggtaaaggt tgatgaatca acggtgatcg tttcatccat
    11281 atggccccac atctgcgcgc cggtaacgtc cgggctggtt ggggtagtaa aaataacgct
    11341 gtagttaacg gtattcggca ggtaggaatt taccggcgtt acgctcagca ccgtttttag
    11401 cccgacgccc tgtggttgct taatctcgac cgtcgccacg ccccgggcat cggttattcc
    11461 cgcataaacc ttcgatgact gtgatgtgcc gtattgcgtg ttatccatcg tgattgcacc
    11521 actgctcggg tcatcaaacc cgctcacctg ccccgctcgg ttatacccca tacctttggt
    11581 aatggtgaat gcagtaaagg gtacagggat attattgagc gagttaatcg tacgcacggt
    11641 catcacaatg gtgtcaccca ctttggcttg cgcttgggtg ccaacagaca catcatgatc
    11701 ggtgctgact tcaacattcg ctaccatctc attacccgaa caggagaggt aattcggctt
    11761 aaagccggaa tagctgtcga cgctcccgtt ccccagatcc accgatgaat gcgtggcttg
    11821 ctccaccgcg cttaaatagc cctgttgtgc agtgggccag ccatattcag taccaatcgc
    11881 gttaccggga tgggctgaaa acaggctttc cagtgagctc tggcgcggta tatgaccgac
    11941 gccgcattcc gcctgcatac tggtgttttg atcgtagagc gcccagtctt cgttattttc
    12001 ccgcactgtt cccaattcat gctcggtttc atccgccagc aacgggcgtt tatacaaact
    12061 gccggactca ataatgccgc gcatatggcc ccacatccgc gctttgtcgc tgtccgggct
    12121 ggtgattacg gtaaaaatca catctttggc atctgtggcg ttaaaatcgc tgcgcattct
    12181 tgccgtgata tgggttttca cccccgcgcc gccgggctgg ctcagggtga gcgaagcctg
    12241 gccgttggcg tcggtcatgc cttcataggt atgtgcgtcg acctggcgaa aattactgcc
    12301 gccagcaatc tgtaccggat gggcttccca ggcagtgtcg gtttgattct tacgattgac
    12361 gccatcatcc agatgtagcg agaagtagta atacgccagc ggttgatcgt tgtttttcgt
    12421 gtcggtgatg gccagacgca tcgtcgcatc ctcgcccacc ttcgctttca tcgcctgcgc
    12481 cgtgtcatca tagttttctg gcgtgaggac gattttcggc tcgacgtcag gcgcgggctt
    12541 atccacgcag ctcacgagga acgtggtgct gtccggcttc tgcgtttcgc cacggttaga
    12601 gacatctgcc gcatggtgct gcccggtaag ccccgccagc gatgaccagt aataatcgcc
    12661 ctgcatcggc cagccgagca cagtctgtac cgtggaggga atcaccgtcg ccagtgcgcc
    12721 gaagtggcgc atgcccggca gcacggtgca atggctatct gcgccgctcc aggtaaaggt
    12781 ggaccaggtt tcattgtgat cgacgaccgt ggcattttcg ttactgactt ccgccgccag
    12841 ttttggtcta ctgaacgtgt aaccatgggc ttccaccgtc tccgccatat ggccccacat
    12901 cgtggcctgc gccacgtccg ggctggtgag cacggtaaag atcaccgccg cctcactggt
    12961 ttgtgcaatg ccggaaatgc cgactaccag cggcgtttta acaccggggc cgttaggctg
    13021 ggtcaccgtg atggttgccg taccgttagc atccgatgtg ccacgatact cggttaccgt
    13081 cgtcgttaac tcggtgctat ccagcaccac cggcgcggta ttatttaccg cgccctgacg
    13141 gttttgggca tctttacgct tgataataaa tggaatatta cccgcgacgt taccctgaca
    13201 atctttggtg gtgacggtca gcgtgatagt gccgccaacg gtggtttttg ccgcgccgag
    13261 cgtttcgtta taaccactgg cgctcaccga aattaaatcg ccctgcgcgg gcgcgatatt
    13321 cagcgtcact ttctggtcag ccagcaccac cgtcccctca tagggataac cggtcgaggt
    13381 cagcagataa agcagtggaa tggtctgctg cgcatcaatc tgtggccagg ccaccagcga
    13441 acgcccggcc agaccttgcg cgcaaaagct gcggttggcg atgggctgtg gattgagcgg
    13501 cgtatccggc gtggcggcat ccgcccacac cagactgacg gtcggcggcg tctggttttc
    13561 ccagcgcagc gcaggtggtg tagaaaactg gtcgccttca ctgtcctgag gattagcaac
    13621 tatcaggtta gcagcgtcat cgtcaacgct aaattcattc ggtttagcag aaaacgccac
    13681 atcatgagat gcagcaggat caagctgaac gcttccctga taaacgggta cgctggcgct
    13741 atcggcaata ggaactgtac cgttaaactc gcctgtagaa ccaggatatg tctgccattc
    13801 cacctctgcc cgtgcaaagt cagacagcat aaaggctgcc aacatgccca gaagcacaat
    13861 gatttttcct ggtttcagac ataccgacat gtagaaaact ccagcatgat aaacaaaacg
    13921 tcaacccgtt ttgcttaacg cgtcgctaag caaaacaggc ggaaaacagc attcccatac
    13981 gggaatgctg ttgtgcgcat tagtggtgtt tgtaaatgat agacagcgta tagccctgaa
    14041 cgccgtcgcc gtttgccggg attgcaaggc cggtcttacc attacggtca ctaccgttaa
    14101 ggctggttct tgcttgttcg ttagtttgcg gaataacaat gtcattgtta tcgaagctgg
    14161 cgttggcaat accgcccgcc gccgcaagtt gtttactgtt accgttaaag acccacgcaa
    14221 agtcgtagtt agcggtaacg tccacatccg tacccgcgtc gtatttaccg ttctggtttt
    14281 catccgagta cagtttggcc acatacgttt tgttaagcgc aatggctgta ttaccaccga
    14341 taaggttggt actggtgtct ttctcgaaga tggcaacttt caggttctgg ttaacaaccg
    14401 gaccaggatc gacattatcc gtatcgctgc cgccaccgct ggccgtagaa atatccagca
    14461 gagtcagcga agttccctgg aagggattac cggtctgagt gataggctgg atttcaacac
    14521 cgatatagcg tccacgatcg ccatcggtga ttttataact ggtttttcct gccgccgcag
    14581 tcaactccgt tttaccgcca cccgcattat cactgtagct gtaccatttg atggtcgccg
    14641 tcgttttcgc atcaatgcct gcggaaccat catcgatatc cccttcggta tcgccgatat
    14701 cccaacccag cgtgatggta tcccctgagt aaaggcgttt atcagactcg ctacctgcag
    14761 gacggatacc tttactatca gatgtcactt tgatgtgatc cgcatcttcc gagttaatcg
    14821 tagcattccc cccttcgcga tagagccagg gtatggtccc ctcgaattca gctgtcgttc
    14881 cactggcgac ccaggcagca tcttccgcca tagcgactgg tacggcacag tatccagcca
    14941 gcatcagtgc cagcgcgact tttgtcaggc cacgtttcat atgaggtttc ataaacactc
    15001 tccttggtta acattcagcg gtggcccgat cagccatggc gctgttccta tgtcttgctg
    15061 taattgacct gcaaaccgta cccttgcaca ccatcggtta cctgggcact aaacttcgct
    15121 gccgcttcct tgttcgttgc cgggatctcg atatcgacgt tagtggtatt ggcttgcgcg
    15181 ccagtattgc catttacgct atgccccgtg aattcccatt ggtagtcata ctgtttcatc
    15241 tcttcgtcag tgagcttttc agatagctgg aacacaccgt ctttgttagc gtcgcgccac
    15301 aacacaaagc gatatcggct attgaggggc aacggttctt tattgccgat aagattcacg
    15361 gatttagccg caccaacgcg atagataaag gcattcagcc cgccgatgtc agcccccagg
    15421 aacgttacgt caacatagtt actgtcatca tagggggcag ctttcgcctt ccaacggaag
    15481 gtgccaggca acgtactgga gagcgtaacg gtcccctttt ctttatcttg tttggcgata
    15541 aagacacagg gagtctcggt agtgcagggg ttattcgtca gcgtcacttt tttatcgttg
    15601 gcatcaaaca gctgcgggtt gagattatcc tgcggcaaga cattaccgtt gcggtcagtg
    15661 ttgtcgccaa agctatcagt aatatcgaga tccatcacca caccatctac accatcagcc
    15721 tgtgcactgg cttcgtggct gtggttgttc tcttccggca cgttcgcgat gttattaaca
    15781 atcagctcaa ttttacggtc ctgctgaacg gtgatttcca ccacgcctga gttctgtcgg
    15841 ttgccctttt catctttcag cgccaggcga acccgccagg tattgagttt tccttgttcg
    15901 gctttgtcag agctgtactg ccagacgggc agcaccaggt tccagtggtt attgttgccc
    15961 tctgtcaggg tattctcttt gataaccggc atcaccagcg tgcgggcggc attgtttttc
    16021 gcctgccatg acacagcgac aattttgtag tggtggttga caagcgcgtt aatggtccat
    16081 ttacattttt ccagcccgat agcttcttct ggcgtatctt taatcacgca ctcgggatgt
    16141 tcattcatcg catcagcttt cagccacaaa gtgacatcga ggggatcttt ctctttatat
    16201 tccagaacaa taaagttgtt acgttcaaca aagtcgtgac ggctgcccat gagtgagtga
    16261 gccgcgctca ctttgtcagg atcaagctgg tctttgagcg ggacgccaaa ctggtagtta
    16321 agcgttgcgt tgaccgtggt atcggtgtta tcaccggttc ctgctttgaa gtccgttcct
    16381 acagcaatga gcggaacagg ctggtatttc acgcctaacg tgacagcgaa gggatctttc
    16441 tctagattgt cggtaccaaa tagcgcaact tcatccccgt aataatgttc gaaaacgatt
    16501 ttaccgccaa gctgcggata agcgggaagc caggcttcgg cacgaatatc ccaaccgctc
    16561 gcagggcgtt cttcataaaa gtcgaaatct tcagagtctt tccagtcaga aagcggatga
    16621 taatagttac cagaaaattt taaataatca gtccacgctt cagcgcctat acctaaacgg
    16681 cgatgtccac gggtaaaatc ataatcataa aatatatttc cacccaataa ccatttatca
    16741 aaattatgtc ttacaccaag ccctatattt ccgatcgtac gatcttcttt tcgttgcgca
    16801 gaaaattggc tgaaataaac agtggtttga ttatcgtacc agggaacaaa ataatcgatg
    16861 gaactgccat ccagatcgcc gccctgacca atggagaggt tcgaacggac cttaccgtag
    16921 ggagagagtg tgtcttcaat ataagattgt gcggttgagg tgatttgact ttgcagatag
    16981 cttctggcct gagattccag cgcctcaggg gtcaagtttt caaacccttg cgtggcggag
    17041 ttgatgacgt aatcggctcc gcgctctttc agcgatttac ccttgttggt ttgttcatcc
    17101 tgttgtgctg cctggctacc taaatcaggt aacgattcgt tttgcttaac atttatctct
    17161 ggtgccgcga aa
//
LOCUS       DAJOTB010000102        16937 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJOTB010000102 DAJOTB010000000
VERSION     DAJOTB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.102"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(160..1089)
                     /gene="pfkB"
                     /locus_tag="OHN28_003305"
     CDS             complement(160..1089)
                     /gene="pfkB"
                     /locus_tag="OHN28_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCQ2298643.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1375..2133
                     /locus_tag="OHN28_003306"
     CDS             1375..2133
                     /locus_tag="OHN28_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCQ2298644.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(2186..2293)
                     /locus_tag="OHN28_003307"
     CDS             complement(2186..2293)
                     /locus_tag="OHN28_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298645.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(2464..3488)
                     /locus_tag="OHN28_003308"
                     /pseudo
     CDS             complement(2464..3488)
                     /locus_tag="OHN28_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            4012..5940
                     /gene="thrS"
                     /locus_tag="OHN28_003309"
     CDS             4012..5940
                     /gene="thrS"
                     /locus_tag="OHN28_003309"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCQ2298646.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5944..6486
                     /gene="infC"
                     /locus_tag="OHN28_003310"
     CDS             5944..6486
                     /gene="infC"
                     /locus_tag="OHN28_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCQ2298647.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6583..6780
                     /gene="rpmI"
                     /locus_tag="OHN28_003311"
     CDS             6583..6780
                     /gene="rpmI"
                     /locus_tag="OHN28_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCQ2298648.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6833..7189
                     /gene="rplT"
                     /locus_tag="OHN28_003312"
     CDS             6833..7189
                     /gene="rplT"
                     /locus_tag="OHN28_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCQ2298649.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    7306..7430
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            7312..7356
                     /gene="pheM"
                     /locus_tag="OHN28_003313"
     CDS             7312..7356
                     /gene="pheM"
                     /locus_tag="OHN28_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCQ2298650.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7640..8623
                     /gene="pheS"
                     /locus_tag="OHN28_003314"
     CDS             7640..8623
                     /gene="pheS"
                     /locus_tag="OHN28_003314"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2298651.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8638..11025
                     /gene="pheT"
                     /locus_tag="OHN28_003315"
     CDS             8638..11025
                     /gene="pheT"
                     /locus_tag="OHN28_003315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCQ2298652.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            11030..11329
                     /gene="ihfA"
                     /locus_tag="OHN28_003316"
     CDS             11030..11329
                     /gene="ihfA"
                     /locus_tag="OHN28_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCQ2298653.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11430..12410
                     /gene="btuC"
                     /locus_tag="OHN28_003317"
     CDS             11430..12410
                     /gene="btuC"
                     /locus_tag="OHN28_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCQ2298654.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            12473..13024
                     /gene="btuE"
                     /locus_tag="OHN28_003318"
     CDS             12473..13024
                     /gene="btuE"
                     /locus_tag="OHN28_003318"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCQ2298655.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            13024..13773
                     /gene="btuD"
                     /locus_tag="OHN28_003319"
     CDS             13024..13773
                     /gene="btuD"
                     /locus_tag="OHN28_003319"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCQ2298656.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13851..14315
                     /locus_tag="OHN28_003320"
     CDS             13851..14315
                     /locus_tag="OHN28_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCQ2298657.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14563..15276
                     /gene="ydiV"
                     /locus_tag="OHN28_003321"
     CDS             14563..15276
                     /gene="ydiV"
                     /locus_tag="OHN28_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCQ2298658.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            15339..16775
                     /locus_tag="OHN28_003322"
     CDS             15339..16775
                     /locus_tag="OHN28_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCQ2298659.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(16779..16898)
                     /gene="hemP"
                     /locus_tag="OHN28_003323"
     CDS             complement(16779..16898)
                     /gene="hemP"
                     /locus_tag="OHN28_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCQ2298660.1"
                     /translation="MRRITSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
BASE COUNT         4257 a         3910 c         4425 g         4345 t
ORIGIN      
        1 aatgtatcct catgcaacat gacggttatt acataacgga caagatcgcc actggccata
       61 gttcacccct ttgttatcac atgacttttg agcatagtcg gagaaacgcg ttgccgacag
      121 gttggtggtg attcccccaa tgctggggga atgtttttgt tagcgggaaa ggtaagcgta
      181 aattttttgc gtatcgtcat gggagcacag gcgtgttccc tggttgagtg tggctgcact
      241 ccccgcagct acgccaaaac ggaccatctc ttcaagagaa gcattttctg ccagtttaag
      301 tgtcatcgcg ccgaccatgc tgtcaccagc gccaacggta ctctggcttt tcaccggtgg
      361 tggcaccacc tgaatgcagt tttcactatc aacgcccagc gccccttgtg gacccaggga
      421 aacgacaacc cgtttggcct tgccgctatt aacgatttcc tgcgcggctt tgcggacatc
      481 gtctggctgg gtgagttcgc gatttaccag cgcactgagt tctttgtggt taggcttaac
      541 caactcgatg ttaccaatcg ccagtgctgc acttaacgct tcgccagaac tgtcgacgat
      601 gcagcggatc ccttgttttt gcgcagcgga aatcagttgg gttaattttt ccagcttcac
      661 acctggcggc aggcttccgc ttatgaccag gatggccccg gattcaattt ccagaacttg
      721 ctcttcgagc tggcgaaact catcttcatt taatgccgcg cctggcataa caaaacgata
      781 ctgctcaccg cttgcttcca catgtacgtg taaattctgc cgggtccagt ctttggcttc
      841 tacagtagcg acggggacat tttcatccgc caacagtgaa accagatgtt cgccggtcgc
      901 gccacccgcc gggaagatcg ctgtggcagt gcctcccaga tgggcaatgg cgcgggcgac
      961 gttgatgccg ccgcctccgg gttcgaatac cggtgcggta cagcgcagtt ttccttcggg
     1021 ataaatttgc ggggtaattg ttgcgctatc gagtgagggc gcaagtgtca acgtatagat
     1081 acgtaccatc atttcctcct ataggctgat ttcagtctgg caccgaatca gcggaaagtg
     1141 aagaaattaa caatatgatt tattggagct ttacttaaaa atttgcagat aaatatatat
     1201 aaataaaaat ctgttttcgc cacatgctta ttcaaaaaat gaaataagaa tacattgcta
     1261 agttattcgc gcctttttat aattcgttac ctttccaggg acgctaatct ttatccttaa
     1321 gaaagtttcc gggaccgcga tggtctcttt ttttgttgta cggactcttg ataaatgaag
     1381 cttttgaaga cagttcctgc catcgttatg ttggcggggg gcatgtttgc gtcactgaat
     1441 gcagctgccg atgattccgt ttttactgtc atggacgatc ctgccagcgc taaaaaacct
     1501 ttcgaaggta atctgaacgc aggatatctt gcacaatcag gcaatacgaa aagctcctca
     1561 cttacggctg acaccactat gacctggtat ggacaaacca ctgcctggtc gctgtggggg
     1621 aacgccagta atacttcttc taatgatgag cgttcttcag aaaaatatgc ggcgggcggg
     1681 cgtagccgtt ttaatttaac tgattatgac tatttatttg gtcaggcaag ctggctgaca
     1741 gaccgttata acggctatcg agagcgcgat gtgttgaccg cgggttatgg tcgccagttt
     1801 ttgaatggcc cggtacacag tttccgcttt gaattcggcc ctggtgtgcg ttacgacaaa
     1861 tataccgaca atgccagtga aacccagccg ctgggctatg cctccggggc ttatgcatgg
     1921 cagttgactg acaacgctaa atttacgcag ggtgtttcgg tctttggcgc ggaagacaca
     1981 acgctgaact ccgaaagcgc attaaatgtt gctattaatg aacattttgg actgaaggtg
     2041 gcttacaacg ttacatggaa ctctgaacct ccagagtctg caccggaaca taccgatcgt
     2101 cggacaacct tatcgttggg ttacagcatg taaaattatc tgccggaccg tgttatgcgg
     2161 tctggctgtt ttcgtttgtc actgctcagc cccaaatcat tgctgcccaa cgtagcaaca
     2221 acatagcgcc acagattgcc agtagcacca ccaccatctt ccagtgtttt ttatcaaagc
     2281 gtttagtcgg cataccagtg tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa
     2341 agggacaggc atcaagggat tttttgttat tacagtcgtc gggtggcgtt gtacacctgg
     2401 tcactgtttc tgcattttat gaaaaaaatt atgtatcgat atacaaatat cataaatagc
     2461 gggttataaa tacgtcttta gttcatcagc actaaaactc aggctttggc tgattacatt
     2521 tatagcagca taatatgttt ctacagtttt atgatgatta tgatgaatgg ctatatgaac
     2581 agctgacatg ttgtcatgat ttttagcggc caacagtgta aataactgac gttggctaaa
     2641 agaatatttt tttcaaaagt acccaacgtt gatatataag ataacacgac atctttactt
     2701 actgacaaag tatgattgtc tatatataaa atcgcggaca tatgactgac attgtttgcc
     2761 atcattcgac agaagaaaat atctaaagca aagccaaaat cctgactcgc gataacgcga
     2821 ctccttgcaa gaggatggcg aagtaatgct ctgaattccg cgtcccggac taagggaaca
     2881 tgctgctgta tggattcaat ggaggagtaa tgtttaatca ggttgacttt gccttttcca
     2941 tttctcaatg aatcaagggc gtattgtaat gacggatggc gacaaaaaat agtgtcagaa
     3001 ggagattgcg aaaaaataca ctatcattga gatttactgt tggctgtatg gttttttctc
     3061 tatgaatttt tttggacagt ttcatgcaat ggttacgttt ttcggcagtt aactgttccc
     3121 gttcaatata atccagtatg cttcgcaaat ggttagcttt aataggcgtg gtttccagaa
     3181 ataaggaaat gatttatttt ttatagtgta atgagtgtat tttctgcatg gatgggtgaa
     3241 gtggcaaagt taatgataaa aagggcagtg gaataacaat tgtgtgatat ttttcgacag
     3301 cttacggtaa attcctgagt aacacagtat gacagcatcg aacttaaatc agaggtatca
     3361 agtagggtga tgagctggcg cgcaaattga ctaataggct ctgatgaagg cgttagtatg
     3421 tcgctatcga tgtgcattct aagaggagac tcataatgag ttctgataat gatatcgttt
     3481 tcactcataa tctattcgcc ttatatatgt ctggagattt tgaggatgat gattaattat
     3541 tatcgccaga acgaaatgcg cacgaaaaat tgctataaga attgtgtcac ttttatcatt
     3601 gcgatatgac cacttcgtca ctgaatctca tgatcgttta aataccaccg ccaaataacg
     3661 caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgtagt aaacctttca
     3721 gacactctgt gatgatatca gtggttcttt aagcgttttg ctggtgtact cactacaaac
     3781 gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa
     3841 catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg
     3901 aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt
     3961 tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatatat aatgcctgtt
     4021 ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt
     4081 gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa
     4141 ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa
     4201 gacgaagaag gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcacgcgatt
     4261 aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat
     4321 tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg
     4381 atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcatgaa
     4441 gcgcgtgaaa ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac
     4501 atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc
     4561 ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg
     4621 gcttactggc gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg
     4681 gcagacaaaa aagcgcttaa cgcttacctg caacgcctgg aagaagccgc gaaacgcgac
     4741 caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg
     4801 gtattctggc acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct
     4861 aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg
     4921 tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac gtcttctgag
     4981 aaccgtgaat actgcattaa gccgatgaac tgcccggggc acgtacaaat tttcaaccag
     5041 gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt
     5101 aacgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggttttac ccaggatgac
     5161 gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta
     5221 gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt
     5281 cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt
     5341 gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt
     5401 ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta
     5461 cagctggact tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag
     5521 cgtaaggttc cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt
     5581 atcctgaccg aagaattcgc tggtttcttc ccgacctggc ttgcgccggt tcaggttgtt
     5641 atcatgaata ttaccgattc acagtctgat tacgttaacg aattgacgca aaaactatca
     5701 aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc
     5761 gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga ggtggaatca
     5821 ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa
     5881 gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa
     5941 ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt
     6001 cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg
     6061 agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac
     6121 gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagtaag
     6181 tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt
     6241 cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa
     6301 gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa
     6361 atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa
     6421 tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa
     6481 cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg gcttcacagg cattgttcgc
     6541 ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc
     6601 gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac
     6661 gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg
     6721 aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa
     6781 gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac atatggctcg
     6841 cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg
     6901 ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg
     6961 tcagtatgct taccgcgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc
     7021 gcgtatcaac gcagcagcac gtcagaatgg tatttcttac agcaaattca tcaatggcct
     7081 gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa
     7141 agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa
     7201 gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg
     7261 tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct
     7321 gctattttcc gcttcttttt ttactttagc acctgactcc aggaggctag cgcgtgagaa
     7381 gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt
     7441 tgaaaaataa aaagcctgtc tcatcaggcg taattgtcgc agccagtctg gacacggcca
     7501 gcgcggagaa accggaccgt acttaagtac gtgaggattt cgagcactgc ccgggaccaa
     7561 aatggcaagt aaaatagcct gatgggatgg gctctaagtc caacgaacca gtgtcaccac
     7621 tgacacaatg aggaaaaaca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc
     7681 cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa
     7741 aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc
     7801 ggcagctggt gcggttatca atgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg
     7861 taaagcggaa ctggaaagcg cagcactgaa tgcgcgtctg gcggcggaaa cgattgatgt
     7921 ctccctgcct ggtcgtcgca ttgaaaacgg cgggctgcat ccggttaccc gtaccatcga
     7981 ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga
     8041 agacgattat cataacttcg atgctctgaa cattcctggt caccaccctg cgcgcgctga
     8101 ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct ctggcgtaca
     8161 gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgcgttta
     8221 tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt
     8281 tgataccaac atcagcttta ctaacctgaa aggcacgctg cacgacttcc tgcgtaactt
     8341 ctttgaggaa gatttgcaga tccgcttccg tccttcctac ttcccgttta ccgaaccttc
     8401 tgcagaagtg gacgtcatgg gtaaaaacgg aaaatggctg gaagtactgg gctgcgggat
     8461 ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc
     8521 cttcgggatg gggatggagc gtctgactat gttgcgctac ggcgtcaccg acctgcgttc
     8581 attctttgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg
     8641 aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgacgcgctg
     8701 gcaaatcaaa tcacgatggc gggcctggaa gttgatggtg tagaaccggt tgccggtagc
     8761 ttccacggcg tggttgtggg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa
     8821 ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgcggtgcg
     8881 ccaaactgcc gtcagggcct gcgtgtggcg gtagcgacca ttggtgctgt tctgccgggt
     8941 gatttcaaaa ttaaagccgc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc
     9001 ttctctgagc tgggaatttc tgacgatcat aacggcatta tcgagctgcc tgcggatgcg
     9061 ccgattggca ccgacatccg cgaatacctg aaactcgatg acaacaccat cgaaatcagc
     9121 gtgacaccaa accgtgccga ctgcttaggt atcattggtg ttgcgcgcga cgttgccgtg
     9181 ttgaaccagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac
     9241 acgctgccga ttgcagtaga agcgccggat gcctgcccgc gctatcttgg ccgtgttgta
     9301 aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc
     9361 gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtgctgct cgaactgggc
     9421 cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca
     9481 aaagagggtg aaacgctggt attgctcgac ggtactgaag cgaagctgaa tgctgacacc
     9541 ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatatttgg tggcgaacac
     9601 tctggcgtga atgacgaaac gcaaaacgtt ctgctggaat gcgctttctt cagcccgctg
     9661 tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca ccgttatgag
     9721 cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc
     9781 gacatctgcg gtggtgaagc gggtccggta attgatatca ctaacgaagc aacgctgccg
     9841 aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg ccatcatatt
     9901 gctgatgagc aggtaacaga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa
     9961 gacgagtggc aggcagttgc gccgagctgg cgtttcgaca tggagattga agaagatctg
    10021 gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca
    10081 agcctgatta tggggattca ccgtgaagct gacctgtcgc tcaagcgcgt aaaaacgctg
    10141 ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag
    10201 cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg
    10261 tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac
    10321 cgtcagcaga atcgtgtacg cattttcgaa agcggtctgc gtttcgtgcc agatactcag
    10381 gcaccgttgg gtattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac
    10441 gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgatctt
    10501 gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat
    10561 ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg tattggtttt
    10621 gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg
    10681 ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct
    10741 cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg
    10801 gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt
    10861 gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc
    10921 ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt
    10981 gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac
    11041 aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa
    11101 agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt
    11161 gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa
    11221 cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg
    11281 gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac
    11341 taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa
    11401 cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac
    11461 aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc
    11521 tttgcgccgg tgaacaatgg atctcgccag gtgactggtt tactcctcgt ggcgaactgt
    11581 tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt gctgcgttgg
    11641 ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac
    11701 ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc
    11761 aactccccaa ctgggcgctg gggctgtgtg cgattgctgg cgcgcttatc atcactttaa
    11821 tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg
    11881 cattagggat tatctgtagt gcactaatga cgtgggctat ctacttttcc acctctgttg
    11941 atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa
    12001 gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccgg
    12061 tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct
    12121 ggcgcaatgt gttggtcgca gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg
    12181 gtgctatcgg ctttattggt ctggtgatcc cacatattct ccggctgtgt ggtttaaccg
    12241 atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg
    12301 atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa
    12361 cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag ccgcaaaaag
    12421 acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga
    12481 ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta
    12541 cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata
    12601 tgagcagttg gagaatattc agaaagcctg ggccgatcgt ggttttgtgg tgctgggatt
    12661 cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg
    12721 taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg
    12781 acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga
    12841 gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat
    12901 tttatggaat tttgagaaat ttttagttgg cagggacgga ttggtcatcc agcgtttttc
    12961 cccggatatg acgccggaag atcccattgt gatggaaagc attaaactgg cgttggcaaa
    13021 ataatgtcta ttgtgatgca gttacaggat gttgcggaat ctacccgcct ggggccgctt
    13081 tctggcgagg ttcgggctgg ggagatcctg cacctggtgg ggccgaacgg cgcgggtaag
    13141 agtaccttgc ttgcacgaat ggccggaatg accagcggta agggaagcat tcagttcgcg
    13201 gggcaaccac tggaagcatg gtctgcaaca aaactcgcgc tgcatcgcgc ctatctttca
    13261 caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac
    13321 gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa
    13381 ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg
    13441 gtggtgcttc aaatcacacc acaagctaat cccgcaggcc aattgctgct tcttgatgag
    13501 ccgatgaaca gtcttgatgt tgcgcaacaa aatgcgttag acaaaattct gagcgcgctg
    13561 tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac attgcgtcat
    13621 gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag
    13681 gtgctcacgc cggcaaatct ggcacaggcc tatgggatga attttcgccg tctggatatc
    13741 gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga aaatcgggta
    13801 attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct
    13861 gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc
    13921 caaatgccag gctttctgat tcgatcaccg ttattgccgg tttgaacgac cagctacaaa
    13981 gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg
    14041 gatttgtggt tgtgacgatg cgcgatcgtt tcgatttgca gctgccccgt gaaaccaaac
    14101 aacaagcctc tattggcacg caaattgata aagacgagtt gctgccaggt gacctggtct
    14161 ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc
    14221 aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt
    14281 ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaaatgg acggcgatga
    14341 tacatcgccg tccatttact cctgaataca gtagtatttt gcagacagaa attccgtaaa
    14401 tggattattc gtttataatc aggataatct aaaaaaaagg acccctgaaa aagtggttaa
    14461 catggaacaa atgaaagtta ctccttctgt aattgttatg tcttatggcc tcatcccttt
    14521 tagccggata ctgaaaaaca tccttcgaga gggacggtta ccatgaagat ttttttggag
    14581 aatctttatc attctgattg ctatttcctg ccgataagag ataaccagca agaccttgtc
    14641 ggcgttgagt taattaccca tttctccagt gaagatggta cggttcgtat cccgacgagt
    14701 cgggttatag cgcaacttac cgaggagcaa cattggcaat tattttctga acagttggaa
    14761 ttactaaaat catgccaaca cttttttatt caacataaac tctttgcctg gcttaattta
    14821 acgccacaag tggctacatt attattagac agggataatt tcgcaggcga actattaaaa
    14881 tatccgttta ttgaactgtt gattaatgag aattacccgc atttcaatga aggaaaagat
    14941 aatcgcgatc ttttatcctt atcgcaaatg tatccccttg tgttaggtaa tttgggagca
    15001 ggtaatagca caatgaaagc ggtgtttgat ggtcttttca cccgcgtcat gctggataaa
    15061 agttttattc agcaacaaat cacgcatcgc tccttcgaac cgtttattcg tgcgatccag
    15121 gcgcaaattt ccccttgttg caattgcatc atcgcggggg gcattgatac gccagaaata
    15181 ttggcacaga ttataccctt tgatttccat gctctccagg gatgcctgtg gcctgcggtt
    15241 cctataaatc agataacgac gttggttcag cgataaccct cctgtttgct ggtgtttagg
    15301 acgagagtaa ccgtctacac tatcaaacag gaggatctat gaccctgtct tttattaccc
    15361 gctggcgcga tgaattgcca gaaacctata cagcactttc ccctacgcct ttaaataatg
    15421 cccggctgat ttggcataat accgaactgg ctaacacgct gagtattcca tcgtcgctgt
    15481 ttaaaaatgg cgcaggtgtc tggggcggcg aaaacctact gcctggtatg tcaccactgg
    15541 cgcaggttta cagtggtcat cagtttggcg tctgggcggg ccaactgggt gatgggcgcg
    15601 gcattttact cggcgaacaa ctgcttgctg atggcactac aatggactgg catctgaaag
    15661 gtgctggcct gacgccttat tcgcgaatgg gtgatggacg ggcggtttta cgttcgacga
    15721 tacgagaaag tctcgccagt gaggcgatgc attatctggg cattccgacg acccgcgctt
    15781 taagtatcgt caccagcgat tcgccagtgt atcgggaaac ggtggagcca ggcgcgatgc
    15841 tgatgcgtgt ggcaccaagt catctgcgct ttggtcattt cgaacatttt tactatcgcc
    15901 gcgagccgga aaaggttcgt cagttagctg actttgccat tcgtcattac tggccacatc
    15961 ttgcagatga tgaggacaaa taccgtctct ggtttaccga tgttgtcgca cgtaccgcct
    16021 cgttaattgc ccaatggcag actgtcggct ttgctcatgg ggtgatgaat accgacaaca
    16081 tgtcgctgct ggggctgacg cttgattacg ggccgtttgg ttttcttgat gattacgaac
    16141 ccggttttat ttgtaatcac tcggatcatc aagggcgtta cagctttgat aatcaacctg
    16201 ccgtcgcgtt gtggaattta cagcgtctgg cgcagacatt gtcaccattt gttgccgtag
    16261 atggcctgaa tgaggccctg gacagctatc agcaggtttt gttgacgcat tatggacaac
    16321 ggatgcggca gaaactgggc ttcatgacgg agctaaaaga ggataacgcg ctactgaatg
    16381 aattattcag tctgatggcg cgagagcgca gcgattatac ccgcacattc cgcatgctga
    16441 gtctgaccga gcagcacagc gcggcgtcac cgctacgtga tgagtttatt gatcgtgcag
    16501 catttgatga ctggtttgcc cgttatcggg ggcgtttgca acaagacgag attaccgata
    16561 atgagcgtca gcaactgatg caaagcgtta acccggctct ggtgttgcgc aactggttgg
    16621 cgcaacgggc gattgaagcg gcagaaaagg gtgatatgac ggaattgcac cgcctgcatg
    16681 aggcgttgcg aaatcctttc agcgacagag atgatgacta tgtcagtcgt ccacctgact
    16741 ggggtaaacg gctggaagtc agctgctcga gttaaaggct acttggtcaa cagcagcttg
    16801 ccagcctgag ttttacggag cagatattct tgtccgtcat gatcgataat cagtttgcca
    16861 tccggaccta acagtgtctg gctggtaatt cgccggatgg gctgcgggga agcggtcttg
    16921 tgattggcat ccgtttc
//
LOCUS       DAJOTB010000103        16742 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJOTB010000103 DAJOTB010000000
VERSION     DAJOTB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.103"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1912
                     /gene="dhaR"
                     /locus_tag="OHN28_003324"
     CDS             <1..1912
                     /gene="dhaR"
                     /locus_tag="OHN28_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCQ2298661.1"
                     /translation="DLKNKWKMFVKHGWVATNSTNHVMLRSWQKCLKHCDPRHWNTPV
                     KASGQTLQTIFSRNEEFIRISQRVVEDHFTLAGDDRLAFLIIDPHGWVLSLNAAGDYS
                     SQLRELGIESGMSWAEDGIGTNVYSLCRETNLYTQLEGAEHFSEQLHCYAMSAAPVID
                     YYGNIHGYIVCIIETTAELVKLKTSYSCATEIANYIYIENEQKLINKVLCQHNAVIEC
                     MDDGFICWNSHSLITMVNSQAQTLLNIDKESLIGQNIRKGFVFPPILNEAITQRNKLS
                     QKQIVLECRGEFIELMVTLRPLSDGSFLLFLHPLDKIRKIAQQQISTNANFTFDSLHA
                     ASGGMKQVLLIARRAIKSISPILINGEEGVGKLSLAMAIHNESEQRDGPFISVDCQML
                     SPENILHELLGSDVGPSPSKFELAHNGTLYLDKVEYLSGEVQSVLLKVLKTGLVTRSD
                     SHRLIPVRFRLITCTSSSLREYVQQGAFSRQLYYEISMNEIEIPPLRKRREDLKQMID
                     DIIDKYQERTRKKMTITPDANSVLLEYRWPGNISEFKNRMEKVFINCNRLVLGLENIP
                     LDIRQNNSSGDDDIPHLTSLAELEMQAIEHTCRVCEWNLTKAAEVLKIGRTTLWRKLK
                     IYNLYPNVEHAD"
     gene            2199..3569
                     /gene="ibeA"
                     /locus_tag="OHN28_003325"
     CDS             2199..3569
                     /gene="ibeA"
                     /locus_tag="OHN28_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative intracellular survival FAD-dependent
                     oxidoreductase IbeA"
                     /protein_id="HCQ2298662.1"
                     /translation="MEFYLEPARNIPVLATTEVLVVGGGPSGIAAAMSAAREGAATML
                     IERFGCFGGMMTTAGVESIAWWRHENTVESGGLAREIEETAKSMGASSPEPQSNSQAI
                     NAERFKLVADAMLEQAGVRRVLHITAVDVIKQGNNLLGVITESKSGRQAILANVIIDC
                     TGDADIAWFAGAPFIKREREELMCMTTVFSCANINKNAFMQNIKSTQPKYGDWGADEE
                     NKNWSYDVHESCRDMFSPYLGKVFAKGKSAGIIPEDVTLGGSWSTVTEYGDANYLNVV
                     SIPAVDCTDVFDLTRAEIEGRKQAMQAIEALRQFQPGFEQAQLKNFGMTVGTRESRHI
                     IGRAQLTENDICNEGRHADSIGVFPEFIDGNGHLKLPLEANYFQIPYGVMIPQQVENL
                     LVCGRAIDADNFAYATIRNMGCCIVTGEGAGTAAAIAIKNNTTVSQVDIQTVQERLQQ
                     NGVKVF"
     gene            3584..4987
                     /gene="nhaC"
                     /locus_tag="OHN28_003326"
     CDS             3584..4987
                     /gene="nhaC"
                     /locus_tag="OHN28_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCQ2298663.1"
                     /translation="MREKPSFYVALTPIIFMMIVLVVGIGVMGWPASVCLLISAAFSC
                     IIAMAKLKYTWDEIQGFIIDKISAVMAPVLIVIFVGFMVATWSYAGTLPMLVYYGMLL
                     VAPAWLYAIAFFLNAVLSYVSGASWGSVASIGVALMGIGSGLHADLPILAAAIVTGAY
                     FGDKLSPLSDTTNLTSAVTKTKLYDLIKYLLWTTIPSTIISLLFFTWLGMIHTSTGSI
                     SPESMQMLVQLKELYKFGLLPLLPMLIVLCFTFLRLPTVPALLSSAFVAVLVGWLYQG
                     FPLAEGIKATMSGFRLTMITDSTTLLPSVKELLEQGGLNNQGGFLSFIICAMMFAGIL
                     TGTGMMDVTLQSAANKIKSAFGAILASGVLAIIINLLTGSDGLNKIVISELMMKKFED
                     LNLSPLVLARTLEDFGTMSAPIIPWSAAGLYMATTLGVPTFSYLPYCVFCFCSMIFAL
                     IYASTGFRLLRFNEAKA"
     gene            complement(5074..5985)
                     /gene="hypT"
                     /locus_tag="OHN28_003327"
                     /gene_synonym="qseD"
     CDS             complement(5074..5985)
                     /gene="hypT"
                     /locus_tag="OHN28_003327"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCQ2298664.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNISQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHILLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHG
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(6050..7222)
                     /gene="iadA"
                     /locus_tag="OHN28_003328"
     CDS             complement(6050..7222)
                     /gene="iadA"
                     /locus_tag="OHN28_003328"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2298665.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(7235..7696)
                     /locus_tag="OHN28_003329"
     CDS             complement(7235..7696)
                     /locus_tag="OHN28_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCQ2298666.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(7693..8376)
                     /locus_tag="OHN28_003330"
     CDS             complement(7693..8376)
                     /locus_tag="OHN28_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCQ2298667.1"
                     /translation="MTQQGDAVAGELATEKVGVKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8626..9180
                     /gene="kptA"
                     /locus_tag="OHN28_003331"
     CDS             8626..9180
                     /gene="kptA"
                     /locus_tag="OHN28_003331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCQ2298668.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDGEGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIAFTVKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            complement(9193..10371)
                     /locus_tag="OHN28_003332"
     CDS             complement(9193..10371)
                     /locus_tag="OHN28_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298669.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTAQLFKLQVVTS
                     "
     gene            complement(10440..11300)
                     /locus_tag="OHN28_003333"
     CDS             complement(10440..11300)
                     /locus_tag="OHN28_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCQ2298670.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTINGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVKGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            complement(11365..11622)
                     /locus_tag="OHN28_003334"
     CDS             complement(11365..11622)
                     /locus_tag="OHN28_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCQ2298671.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            complement(11619..12386)
                     /locus_tag="OHN28_003335"
     CDS             complement(11619..12386)
                     /locus_tag="OHN28_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298672.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            complement(12396..13547)
                     /locus_tag="OHN28_003336"
     CDS             complement(12396..13547)
                     /locus_tag="OHN28_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HCQ2298673.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(13665..14945)
                     /locus_tag="OHN28_003337"
     CDS             complement(13665..14945)
                     /locus_tag="OHN28_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCQ2298674.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKADLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFLLGNF"
     gene            15130..16050
                     /locus_tag="OHN28_003338"
     CDS             15130..16050
                     /locus_tag="OHN28_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2298675.1"
                     /translation="MTNFTTSTPHDTLFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKYIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGADPEW
                     IQKITGLSAEQMQALEQPLPDREHDSWLES"
     gene            complement(16180..16350)
                     /locus_tag="OHN28_003339"
     CDS             complement(16180..16350)
                     /locus_tag="OHN28_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2298676.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHREKLPSVRSLSQEHGVSTVQQA
                     YQTLETMNLITP"
     gene            16527..16691
                     /locus_tag="OHN28_003340"
     CDS             16527..16691
                     /locus_tag="OHN28_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCQ2298677.1"
                     /translation="MEFHEKKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVQ"
BASE COUNT         4424 a         3940 c         4174 g         4204 t
ORIGIN      
        1 tgatctaaaa aataaatgga aaatgtttgt taagcatggt tgggtcgcga caaattcgac
       61 taatcatgtg atgctgaggt catggcaaaa atgtctcaag cattgtgacc ctcgccactg
      121 gaacacgcct gtcaaagctt ccgggcaaac attgcagacg attttttctc gtaacgaaga
      181 gtttattaga atttcacaaa gagttgttga agaccatttc accctagctg gtgacgaccg
      241 gttggcattt ttgatcattg atccacacgg ctgggttcta tcgttgaatg cagcaggcga
      301 ttattccagt caattgcgag agttaggaat tgaatctggt atgtcatggg ccgaagatgg
      361 cattggaacc aatgtatata gtctttgtcg ggaaacaaat ttatatacac aactggaagg
      421 agcagaacat tttagtgaac agctacattg ttatgcgatg agcgctgctc cggtcattga
      481 ttattatggt aatatccatg gatacattgt atgtataatt gaaaccaccg ccgagcttgt
      541 taaattaaaa acatcatatt catgtgcaac agaaatcgct aattatatct atattgagaa
      601 tgaacaaaag ttaataaaca aagtactttg ccagcataat gctgtgattg aatgtatgga
      661 tgacggtttt atttgttgga atagtcattc tttaattacg atggttaatt ctcaagcaca
      721 aacactattg aatattgata aagaaagttt aattggtcag aacatccgaa aaggattcgt
      781 atttccgccg attttgaatg aggcaattac acaacgaaac aaactatcgc aaaagcaaat
      841 tgtcctcgaa tgccgtggcg aatttattga acttatggtt acccttcgcc cgttaagcga
      901 tggttcattt ttgctttttc ttcatccatt agacaaaatc aggaaaatag cccaacagca
      961 aataagcact aatgcaaatt ttacctttga cagtttacat gcggcttcag gtggtatgaa
     1021 gcaggtatta cttatcgctc gccgggcaat taaatccatc tctccgattt tgatcaatgg
     1081 cgaagaaggt gtgggaaaat tgagtttggc gatggcaata cataatgaga gcgagcaacg
     1141 tgatgggcca tttatttctg tagattgtca gatgctatca ccagaaaata tcttacacga
     1201 acttcttggc tctgatgttg gcccttcgcc atcgaaattt gaactggctc ataatggcac
     1261 cttatatctg gataaagtcg aatatctatc aggggaagtt cagagtgttt tattgaaagt
     1321 attgaaaacg gggcttgtta ctcgctcaga cagccatcgt ttgatccccg tacgctttcg
     1381 cctgattaca tgtaccagta gttctttacg tgagtacgtg caacaagggg cttttagccg
     1441 acagctatat tatgagatct ccatgaatga aattgaaatt ccgccattgc gcaaacgtcg
     1501 tgaagatctc aagcaaatga ttgacgatat tattgataag tatcaggagc gcactcgaaa
     1561 aaaaatgaca atcacgcctg acgcaaattc agttctgctt gagtaccgtt ggcctgggaa
     1621 catctccgag ttcaaaaatc gaatggagaa ggtatttatt aactgcaatc ggcttgtcct
     1681 cgggttagag aatattcctc tggatatccg acaaaataac agtagtggcg acgatgatat
     1741 ccctcatctt acttcactgg cagaattgga gatgcaagct attgagcata catgtcgtgt
     1801 ctgcgaatgg aatctaacta aagcagctga agtattaaaa attggtcgta caacattatg
     1861 gcgcaagctt aaaatctata atctctatcc aaatgttgag catgcagatt gatgcttttc
     1921 aaattgaaat aattttaatg tcgcagtgtt tcattttggt gcagtgcttt aatggtattg
     1981 aaatattgtg atgtgctcca taaatgccaa tgtggtgatt agccagggag acggaaaatg
     2041 tgtttacact cctgaaataa taaaaaacag gcaaagagag atgatctcct tgtatgtctg
     2101 tattagcatg atgttgcttg ttaataatta aaaatatcaa cgcgcttata taagcgcggg
     2161 ggattgtttt actcaattat tgaatacgga gataaagtat ggaattttat ctggaacccg
     2221 ctcgtaatat acctgtactg gcaaccacgg aagtgttagt tgttggtggt ggtccatcgg
     2281 gtattgccgc agcaatgagt gccgctcgtg aaggcgcagc tactatgctg attgaacgtt
     2341 tcggttgttt tggcggaatg atgacaacgg ctggcgtcga gtcaattgcc tggtggcgtc
     2401 atgaaaatac ggtagagtca ggtggactgg cacgcgaaat agaagaaacg gcaaaatcaa
     2461 tgggggcgtc cagccctgag ccgcaatcga atagtcaggc tattaacgcc gagcgtttca
     2521 aactggttgc ggatgcaatg cttgaacagg caggtgtgcg ccgcgtacta cacattaccg
     2581 ccgttgatgt tatcaagcag ggcaataatt tactcggcgt aataacagag agtaaatctg
     2641 gtcgtcaggc tattttggca aatgtcatta ttgactgtac tggcgatgct gatattgcat
     2701 ggtttgccgg agcaccattt attaagcgtg aacgcgaaga gctaatgtgt atgacaaccg
     2761 tttttagttg cgcaaatata aataaaaacg cgttcatgca aaatattaag agcacgcaac
     2821 ctaaatatgg agactggggg gcggatgaag aaaataaaaa ttggtcttat gatgttcatg
     2881 aatcttgtcg cgatatgttt agcccttatc tgggcaaagt ctttgcgaaa ggaaaatcgg
     2941 cagggattat tcctgaagat gtgacgttag gtggttcctg gagtacggtc accgagtatg
     3001 gtgatgcgaa ttacttgaac gttgtcagca tccctgccgt tgattgtaca gatgtttttg
     3061 acctgacgcg tgccgaaata gagggccgca aacaagccat gcaggcgata gaagcgttgc
     3121 gtcaattcca gccaggattt gaacaggcac aattaaaaaa tttcggtatg acggtgggaa
     3181 caagagaatc aagacatatt attggtagag ctcagcttac ggaaaatgat atttgtaatg
     3241 agggacgtca tgcggattca ataggggtat tccctgagtt tatagatgga aatggtcatc
     3301 ttaaattacc tcttgaagcg aactattttc aaatccctta tggcgtaatg attccgcagc
     3361 aagttgaaaa cctgttggtt tgcggacggg caatcgatgc agataatttc gcctatgcga
     3421 caatccgtaa tatggggtgt tgtattgtca ctggagaagg tgcagggact gccgctgcta
     3481 ttgccattaa aaataacact accgtttcac aggtagatat tcagacggta caggaacgct
     3541 tacagcaaaa tggcgtaaaa gtcttttaac agggaataaa gttatgagag aaaaacccag
     3601 tttttatgtc gcgcttacac cgatcatttt tatgatgatc gtgttggttg ttggcatcgg
     3661 tgttatgggc tggcctgcca gcgtctgttt attaatttca gcggcgtttt cctgcattat
     3721 tgcaatggct aagctgaaat atacctggga tgaaatccag gggtttatta tcgataagat
     3781 ttcggcggtg atggcaccgg tacttatcgt tattttcgtt ggctttatgg tcgcgacatg
     3841 gagctatgca ggtacactgc cgatgctggt ttattacggt atgttactgg ttgcgcccgc
     3901 atggctttat gccatagcgt tttttctgaa cgcagtattg tcctatgttt ccggggcatc
     3961 atggggatct gtcgcttcta ttggtgttgc attaatgggc atcggttccg gcttacatgc
     4021 cgatcttcca attctggcgg cagccattgt taccggggct tattttggtg acaaactttc
     4081 accgttaagt gataccacca acctgacttc agcggtaacc aaaactaaac tttatgatct
     4141 gattaaatat ttactctgga cgaccatccc atcaacgatt attagcctgc tgttttttac
     4201 ctggctggga atgattcata cttctacggg ttctatttca ccagaaagca tgcagatgct
     4261 ggtacagctc aaagagttat ataagtttgg tctgttaccg ctactgccta tgcttattgt
     4321 tctgtgcttt acctttttgc gtttgcctac agtaccggca ttattaagca gtgcgttcgt
     4381 ggccgtattg gttggatggc tgtatcaagg tttcccgctg gccgaaggta ttaaggccac
     4441 aatgagtggt ttccgtctga cgatgatcac tgattcaacg acgctgttac cttctgtgaa
     4501 agaactgctt gaacaaggtg gtcttaataa ccagggcgga ttcctgtcat ttattatctg
     4561 cgccatgatg tttgctggca tcctgacggg aacaggaatg atggatgtca cattacaaag
     4621 tgcggcaaac aaaataaaaa gtgcctttgg cgctattctg gcaagcggtg ttttagcgat
     4681 catcattaac ttacttaccg gttctgatgg tttgaataag attgttattt ccgaattgat
     4741 gatgaagaaa ttcgaagatc ttaatttatc accgttagtc ttagcccgaa cgctggaaga
     4801 ttttggcacc atgagcgcgc caattattcc gtggtcagct gccggattgt atatggcgac
     4861 aacactaggc gtgccgacgt ttagttactt gccgtactgt gtattctgtt tctgctcgat
     4921 gatatttgcg ttgatctatg cttctacagg tttccgttta ctgcgcttca acgaagcaaa
     4981 ggcctgagtt attcaagata ataaatgcgt ttgccggatg gattaatctc catccggcgt
     5041 taatgtcaaa attgcatgtt ttgcagaata atgtcagcta agcacaatct ccagctcgcg
     5101 cagttcacgc cagaagcgtt cggcaactgg attcatgcgg gtattcatcc ggtatgcata
     5161 ggcctgaatc gggataacta attcgtcccg attcagtaca acgagctgcc cgctgcgaat
     5221 ttcttgttgt atggcgtact ccggtagcca ggcaatccca cagccgtcga gggcaacctg
     5281 ctttaataac tcgctcattg aagacacgaa aaaggtgctg aaacttaact caccgtggcg
     5341 cgtcagggtg cggttaatca atcgccccat gtaggagttg cggctgtaat tcagtaacgg
     5401 aaagtgtggc tgagcgaggt taaaaagtgc ttctccatgt tcgtcactgg cgcagacggg
     5461 gaacaattga gattcaaata agagaatgtg gtcaaacggc gcttccagca aatcttcgtc
     5521 gtgaaaggaa aaaatacaat cactttgccc ttcacgcagt ttatcgaccg cttcatcgac
     5581 atcaatggct tcaattgccc aggtaaagag cggcggcatc tggctgataa tggacggtaa
     5641 cagaccgagg gaaagagagt gtgcagcggc tatcttgatt ttacgttgcg cgtaatcgct
     5701 gccgccacgc agctctgcca gattactttc taactgttgc aacagatggc ggatctgcga
     5761 atggaagatt ttcccttgtt ccgagagttg cagcggcgtt acctggcggt taaacaattc
     5821 aacaccaatc gcctgttcca gtgcacggat gcggcggctg aatgctggtt gcgagatgtt
     5881 gcgactgact gccgcctggg aaaaattgcg acatttttcc agggtcagaa aatcataaag
     5941 ccatttggtt tcaatgttat gcaaaatcgc accacagtca tccattacgt tatactctgt
     6001 tagccgccct tgcgggcatt ctacgtccat tcgggcggct gacaaccgtt tatgccgttt
     6061 caaacgttcc tttcacgcag gctttgccgt ctttgaccat cagtttgccg cgagcgtata
     6121 cctgctcaat gcgtaattca ggcgtcatga ccagtaagtc tgcgtcattg cctggcagaa
     6181 tttcgccttt cccggtcagg ttaaggaaac cggctacgct actggtgagc gggcgcaggg
     6241 catcgctgat actgaaatca tagtctttga ccagcacctg cacggtttcc agcaatgttt
     6301 caaaacctgc aacaccgata tgggttaaat ttccttcgtc atcgaagaac ggctggctac
     6361 cgttgccgtc ggagctgagg gtgacgcgtg ccagcggaat acccgcctga acggcgcggg
     6421 caataccttc ggcaggggcg accggttcgt caatgctgct ggtgatatcg atggtgccgc
     6481 ctttgcgcgc gaactccagc gcctgctcaa acaacggaac gttgcggtta acgtgggtcg
     6541 gcagcagctt gctgatcggc acatcgcagt tttccagcag gtcatagaca ggctgtaacg
     6601 cctttttgct gtcacccatg tggaacacgg tgacgccagg tttaccgccg agcaaaccgc
     6661 caacgcggga ttccgccgcc atattagcca ggtgataaac gtccggtgcg gcagaacggt
     6721 gatcagagat ggcgcatttc acaccaatca cgcgatcgat aatcgccacg tctttttcca
     6781 cggaacccgt aatagtgcgg gaagggacat gataagcgcc ggtcagcatc caggcgctga
     6841 tgccttcttc attgagcgca cgggtcttgg cgagcaggga ttccgggtgg cgagagatag
     6901 agtcggtgcc cagcaggcca accactgacg tgacgcccgc ttccgtcaga cgacttaacg
     6961 ccacttctgg cgtgcgtgtc gtgggacctg cttcgccgcc accaccaatc aaatggacgt
     7021 gttgatcaat aaaacctggg cagaggatct gcccgctgag atcgacaacc gtgcagtccg
     7081 gtacaatgtc agaagggatg ttgctggcaa cggcaataat cttgccgtta gcgacgagga
     7141 catcgcaaat tccccgatct tccggcgcat acaaatgcgc tccctgcagc agggtaaaac
     7201 cggctgcgga ataatcaatc atggtaactc cttgttaaac aataagctgc ataacccaga
     7261 tcgacagcaa tgcgttaatc acgcagaccg taatgatatg cgggtaatat ttggcattca
     7321 cttcggctgt ccccaggcag cgaccgacat tttgcaccgg gttgcccatt aaatacatcg
     7381 cgggcagtaa aacggtgaca tcgtgaccgg ttaatgcgcc agcggttgcc agactggcgg
     7441 caacaccgac tgcgccgccc atactcatta atgcggctaa aaggacggtt gccgcttcac
     7501 cgggcaatcc ccacagcgcc attacgggtt cacagatatg tcccacccag tcgagcaggc
     7561 cagtaatttt cagcgcctgg atgatgacaa aggccatcac cacgtttggc agcaggttgg
     7621 tggtagcgat agtaaagcca cggcgcgcgc catcaataaa catatccatg acgtttttgc
     7681 ggacctgagt tgtcatgctt gcgctccttg cgttgggttg cgacgttctt cgaagttaag
     7741 ccagacgcgc aaaatgttgg ctccgacaaa tttaaacacc aggatgacgg ctaaaggtac
     7801 gattacggac gtgcccagaa aggcaaacac tgcgacgccg gaagagaagt agttggtgat
     7861 gatggcgcta ccgctggttt gataggcggc aaaaatcact ttatcacgtt cggtaatttc
     7921 cccttcctgc gccagttctt tggtcatacc cgcggcggca tcggtgtttt gcaggttagc
     7981 aatcagcgcc agtgaacaga tgcctggaat gcccaacaac ggttttagca cgggcgtcat
     8041 taactgctgc gcggcgcgca atccgccaag accatcggta atcgagataa ttcccagaga
     8101 gagaatcact gacggagcca gttccagcgc gaagagaaag ccatctttag ctcctgtgcc
     8161 acctgcgccg cgaaacgagg tggtagcgcc attcgcacca ggcagttgcc caaacgatcc
     8221 gttcagaacg gaaaagtcaa aaacgcgcca ccaactgtct gtcccggaaa aaactccgga
     8281 aaaaaagata atcgtgagaa aaaaggccag atacccttta acacccactt tttctgtcgc
     8341 cagctcccca gctacagcat ccccttgttg tgtcataaca atccccattg tttttctgtg
     8401 tatgtgtgtt gcttgagcca ggaaaattcc tggagctgaa aaaccttatc agccatgtgg
     8461 gtatggggaa aatgcaagta tgtttcttgc tatgcgtttt ttgcatagcg gaaaaatgac
     8521 tctgtgtggc aaaggttctg cgcaggattg agttgcagaa aattcataaa cccagtgaca
     8581 atgacatcag taaataaaaa cagaaaatcc agggacgaaa acgcgatggc aaaatacaac
     8641 gacaaagagc tggcagagac gagcaagttt ttaagttttg tcctccgaca taaaccagag
     8701 gcgattggca tcgtgctgga cggtgaagga tgggcggata tcgataagct catactctgt
     8761 gcccaaaaag cgggcaagcg gttaacccgc gcattactgg ataccgttgt cgccaccagc
     8821 gataaaaagc gctttagtta ttccagtgat ggtacgtgta ttcgcgccgt tcaggggcat
     8881 tcgacttcgc aggtcgccat tgccttcacc gtaaaaacac cgccacagtt tctctaccac
     8941 ggtacggcaa gccgtttcct tgacgagata aaaaaacagg ggctgattgc aggggagcgc
     9001 cattacgtgc atctctctgc tgatgaagca acggcacgca aagtgggagc aagacacgga
     9061 tcgccagtta ttttaaccgt caaagcacag gaaatggcga aacgaggcat ccctttttgg
     9121 caagcggaaa acggggtctg gctgacatca acggttgcgg ttgagtttct tgagtggtaa
     9181 gggaaacttc cctcatgaag tgactacttg cagcttaaat aattgcgctg tgctgataag
     9241 cgcagcaata aacaacgccg ccgcgccgca gagagtggcg ctggcaagcg ccgatgttcc
     9301 attgccaagc atactggtca gcgccgggcc aagaatttgc ccaatgccgt aaatgagcgt
     9361 cacaaagcct aaaagattga gatttcccgg tacgctcagt tggcgggcga tagtcatcac
     9421 cagagatgtc gtgcccataa aggtaccgcc aaaaccaaga ctgctgatga taagcaagag
     9481 aggcgagtcg ctggcgagag taagcagcac acagatagcc tgcaccagca aattcgccgt
     9541 caggcagggc aaagcccccc aacgttttgc tgcccatagc cagccaaagc aaccaggcac
     9601 aattgataag ccgactaacg tccagagatg ggcggttaac aatggtgagc ctgcatcttt
     9661 cgccatgagc ggcaggtaag tcgcgacgat gatataacca aatcccgcca ggccgtacag
     9721 aatagccagt aaccaccagc tcattatctg ttgctccgtt tttgccaatg gcattggtgt
     9781 gatggcgtgt tttttcgagg gcattaaaag cgtaagtgca atcagcatca tgccagaaag
     9841 cgccccggcg ccttgccata acgtttgcga ggagaggtca aaatgcaggc ctgccagaac
     9901 atattcattg cccagtgcga tgccaatgcc aacgccagaa aacaaagctg ccagcacaaa
     9961 aggatggcgc gtgtgctgca taatcagcgt cgaaccaaaa atcagcatac cggcgctggc
    10021 gacacccgcc aggacgcgaa tcaataacac cagaataaat ggcggcaacc atgccatggc
    10081 gaggatcaac aacccgctag ccagggcaga agccaacaga aatgggcgca ggcgcgaagg
    10141 ttggtgaaat gcgccaaacg aaaatagcag actgccagcc agataccccg catagttgcc
    10201 gctggcaatc cacgagagct ggctaaatga aaacgatcct tccgccatca tgacgggcaa
    10261 cataggcgta taaaggaagc ggcctaaacc catacctaag gtcagaacca gcatcccgaa
    10321 aagcgcggtg ctgaaagaaa agcgttctac gggatgcgag ggcgaaggca tgtttaacct
    10381 tgtttgtcat tttttattgt taacaagtta ataacaaaaa ggtgaaacat gccagcctgt
    10441 taatgcgatg attgaggggt aaagcgataa aaacgatttg gttcgctaac gatataaata
    10501 ttacctgagg catccatcgc aaccccttcg gcctgtttga tgttatgcga caaacctcga
    10561 ctgcctttgg ttagtgacat ttcaccgata acctcgccag ccagcgttac ttcctggagt
    10621 gcgcgagatt catgagacag caccagaagc gtattttttt gttggttaaa ttctgcgccg
    10681 gacacatcat ctaaagtaaa ttgccgttgt agtgccttgt ctttgctgat gtgcaattca
    10741 ttgctactta acagcccttt aactttgtag acctcaatcg ggtttttctc tttaaaaaac
    10801 cagaaagtgt gatcctggcg agaataagcc aatccttcga aaccgcaatt ggttggggat
    10861 tcctgcagcg ggatttttat ttttttgagg atttttactt ccgagtttgg ggtcagtgaa
    10921 atcacataaa tggcataatc gcgctcatcg ctaatgacaa actgattatc gccaatgtat
    10981 tcgatcgtct cgagatcctt aacaaaatcc agaggaattg tgcggatcaa atcgccattg
    11041 atggtcattt caacaatggc ggcaggtttg ttgatagtgc taaaaagagt attgctttgt
    11101 gccgaccagg tcagcgaaga gatattattc gttatgcctg caatttcttt gccgtcaatg
    11161 gtggcgtggt aattctgaaa tgatgctgca tgattgcttt tacgggcgca agattgcacg
    11221 aagaaagtac acactgccac aatgacaaaa agaatcacaa taacgaagat gcgtttactt
    11281 aacgaaatac tttttgtcac ggtaatccct ttaaggcaaa tcaataagat atcgagaagt
    11341 atacgtagcg atcttaatga atccttaacg cggagaaatc ccgtaatgtg caatgcagcg
    11401 ccagccacca tcctgctgac aataaactga ttccgtatgc ccagggatca cccattgaat
    11461 ttcctcgccg gggggacagg tcagccaaat gcataacccg cagccgccac gtaaatcacg
    11521 gggaatatcg ctgacgcgaa aagtcatgcc cgctgcctgc aacgccttgc gggtttgtat
    11581 gacgccgacc gtggagtgaa ataaaaacag atactctttc atgcgcgttt tctctgccgc
    11641 tgaccgatca ccgccgcgcc aattgcacca gcgaattgcg catcagggtg agtttgtacc
    11701 ggcattccca gatggctttc cagcatgtgg gtaaaccttt gacagtgact aacgccacca
    11761 gtaaacagaa tcggcgcttc acaggagaga cgagcaatga aattggcact ccgccgcgcc
    11821 atggcgttaa tcactccggc gagaatcgct tctggcgcga cgcccgctga gcgcaggctg
    11881 atcacttctg attcggcaaa cactgtgcac atactcgtga tggcgtgcgg tatgacattt
    11941 tcggtaatgc tgtcgagttg ctcgacgctg gtgccaagcg tgcgcgagat tacctccagg
    12001 aaacgcccgg tgcccgccgc gcatttgtca ttcatcagga agtcgcacag gttaccgtca
    12061 tcatcaagct gaatcacttt gctgtcctga ccaccgatgt cgattaccgc gcgcgttgct
    12121 ggctcaagaa accgtgcgcc cagcccgtga caggagattt ccgtcacttg tttatcggca
    12181 aaatccacca gttgccgtcc gtagccggtg agcgtcagaa acggcgttgt ctctaaccct
    12241 tcgcgcagag tttcccaggc ttcagtaatt gctgttgccg ggcgaaaggg tgttggaacg
    12301 aggaaacggc gcgtaatcac gccgtctgcc agtaagatcc ctttggtggc ggttgagccg
    12361 gaatcaatgc caatcgaata tgtcactgcc actccttaca gcatctcaat aaaggccgcg
    12421 acacgggtgc tgagctgccc gacatccgag gtggagtagt ctgtttcaat agcgatataa
    12481 ggaatgttgt gctgctggcg cacatgacgt ttaatcgcca gcgattccac cgcgtaggta
    12541 tggcacgcct gcaaaatcac atcaactacg ccatcgacct gatattcttc aaccatctgg
    12601 ctgagcattt gcaggcgctg atcgttcggc gaaacacagg agcagccaat cgccagatat
    12661 ttatccgcca gcgcgtcgta cacatcaccc gtttccgcca cgcattgctc ggtggctttc
    12721 gccccggtgc agttttcata accgacaacc cagccgccat tctcttcaat cgcgcgcacc
    12781 actttctctg ctgcgccgcc aattgggcag ccggtgatta aaatgcgtgg gcgcgggtcc
    12841 agtcgctggc cttcttccca ctgctgacga acgcgggcgg tcattgcatc cagttcattg
    12901 atcaacgcct ctttatcgaa ccggaaggtt gcgccgtaga ctactttcag aatgtcgctg
    12961 ccgctaagcg ccggaggatt taactgcccc aaatgataaa agttggccag tgcgcgacgt
    13021 tcgcggtttt tcagcgcaat ggcatcgcgc agagcatctt cgctaatctc gtgcccaaaa
    13081 cgttcttcta ccgttttttg caagcgcagc atctcggctt tccataacgc gcgtgaggca
    13141 tcgtccttaa cgctgtttgg cagttgcatc acatgaacgg gcttaaactc cgccatgtat
    13201 tcatacattt tctttttgcc gtcgcaggtg gtttcaccga ccaccagatc agaaaagtag
    13261 aagtaggggc atttatcggt tttgccgaag ccgtagctgc ttttaatcag cgggcagagg
    13321 ttgcgcggca gatctttctc ggcttcttca atggtttcat cagaggtgga acagagcgaa
    13381 accacaaccg caccggctgc catcgggatc tcttgcggca taaaggtgca gtaggtgcca
    13441 accagcggaa tgccgcgttc cttgagatcc atgacggtga gaaagccttt ctggcgagct
    13501 tcagagaact gatcgaaaat ggcgggtaga tcggtgacaa gtgacatgat tttccttccc
    13561 cgtaccacgg gagataatga aaagaggcca cattattacc attcttttag tgtggtttat
    13621 ttgatctctc tcgcggttag ccatttagct tttatcatcg atttctaaaa attgccgagg
    13681 aggaacaagg ccgggagctg ggacagcaaa tacaaaataa gtccaataca accgccaacc
    13741 agcgtaccgt tgacacggat aaactgcaga tctttgccga tatttaactc gatttgccgc
    13801 gacatatccc gcgcatccca gctttttacc gtgtcgctga tatggcgcgt caggaatgcg
    13861 gaaaactcag gcgcaacgcg gtgtgcggct tgttccagat gaccatttaa cgacgcccgc
    13921 aaggcatcat cggcaattaa tgtttcgcca aaccattgac ccgcccgcgc gatacgctct
    13981 ttcactcgag aatcttcact gttgatatcc gctttcagcc attcccgtaa atccccccac
    14041 aattcactga gatagcggtt aaaagcttca tcttctttca gatagctttt tacggcatcg
    14101 gctcgcgctg ccatttccgg atcgtttttc agcttgtcga tcagggcaaa agtggcgcga
    14161 tcaaacgcat ggcgaatctg atgcgcacga tcgcggctga tatcatcaag caaagaattc
    14221 accgcgtcag aaaccaactc cgcgctatgt tcgcccagcc attcagtggg caaaattttg
    14281 gctttcagtg gatgctcgct ctccagccag cgaacaattt gctgggcgat aaacttgcgc
    14341 gatttatcgc gctggagaag ggcgatcaac tgtgcgatca gcgtatccag cagcacctga
    14401 tgacgatcgt ttttggtcat actctccagc atcaacgcac tggtgccaga aagatcggct
    14461 ttatcaatcg cccggtggac cgcgcgctta agcaggcgct gaatacgcgc atcatcggtc
    14521 agttcgagaa aaccgctcat gatctgcaac agatgctgac caacgcggcg ggcgttctcc
    14581 ggctgactaa accagttgcc aatcaacaac gccggttcgt gacgtcgaat caatgccacc
    14641 agcgactggg tatcgagaaa tttttcctgc acgaactgac cgagattttc gccaattcgg
    14701 tctttattac gtgggataat cgccgtatga cgagaaatga tcggaatcgg cacgcggcga
    14761 aacagcgcca ccaccgcaaa ccagtccgcc agtgcgccga ccatcgccgc ttcagcaatc
    14821 gccttcacgc cgctcaccca aaaattgggc ggcaaaaaca gcgtaacgac aaaggtagca
    14881 gcggcgataa gcagtaaaga gagcgccaac attttggcgc gtctgagttc aatgagttta
    14941 ttcatatcat aagaatagag cctgacggta agaaaacgca aaaagcgaga tatcttccat
    15001 aaaaatgtat cgtttgcaga agaataaaaa tagcctggaa accgcctcgg ggaataggaa
    15061 atagccaggt gtgaatggct ttgttagtcg ctaaagttgg gccatcttat ctacaggtga
    15121 cggatcgcca tgacaaactt tacgaccagc acgccgcatg acacattatt taaatccttt
    15181 ctcacacact ctgacaccgc gcgggatttt atggagatcc acttacccaa agatttacgt
    15241 gaactgtgcg atctcgatag cttaaaactg gaatccgcca gctttgtcga tgaaaaattg
    15301 cgggcgctac attccgatat tctgtggtcg gtaaagaccc gtgaaggcga tggctatatt
    15361 tatgtagtga ttgaacatca gagccgtgag gatattcata tggcctttcg tctgatgcga
    15421 tattccatgg cggtgatgca gcgccatatc gaacatgata aacgccggca gctaccgctg
    15481 gtcatcccga tgctgtttta tcacggtagc cgtagtcctt atccctggtc tttgtgctgg
    15541 ctggacgaat ttgccgaccc gactaccgca cggaagcttt ataccgccgc gttcccgctg
    15601 gtggatatca ctgtcgtgcc agacgacgag attgtgcagc atcgcagagt cgccctgttg
    15661 gagttgatcc aaaagtatat tcgccagcgc gatctgatgg ggcttattga ccaactggta
    15721 gtattactgg ttacagagtg tgctaatgac agccagataa ctgcgctgtt aaattacatt
    15781 ttactgactg gtgatgaagc gcgttttaat gagtttatca gtgaaattac ccgtcgactg
    15841 ccacaacaaa gggagcgaat aatgacgatt gcagagcgaa ttcataatga tggatatata
    15901 aaaggggaac agcgcattct tcgattgttt ttgcagaatg gggcagatcc tgaatggata
    15961 caaaagatta ccggactttc ggcagagcaa atgcaggcat tagagcagcc cttgcctgac
    16021 cgtgagcacg attcatggct cgagagctaa tatggaacta tcgctatgtg cctccagcat
    16081 atccagcacc tgatcccact gggtaatttc caccggacgc gtcatcgacg gtactggcgg
    16141 ctgggcttta cgttgcgcga caaaataacc cgaacgcggc tacggagtga tgaggttcat
    16201 cgtctccagc gtctgatacg cctgctgcac ggtgctgacg ccgtgctcct gactcaagct
    16261 acgcaccgac ggcaatttct ccctgtgacg atacagccct tgctcaatcc gctcggcaag
    16321 cagagttgcc agatgttgat aacgcgtcat gctgtattcc ttatttggac catacagaga
    16381 gaaaaaccag tacagatgag gctaaaaaac cggatgcaga tgctgtttta acctgtctgt
    16441 atgttaaata aaactaatat ttgcatctgt attgtgaact gccagaagac ggatgatgaa
    16501 gcctctgaaa gatgaggagg cagcagatgg aatttcacga aaaaaaagcc aaaacgccgt
    16561 ttatcagact tgtgcaactc tggcaggcgg tgaggcgttg gcggcggcaa atgcagaccc
    16621 gacgagtgtt acagcaaatg agtgatgaac ggttgaggga tattgggtta cgcagggagg
    16681 atgtgcagtg atagaggatg ttgagaataa atcaggatat taacttttta tatgtaccct
    16741 gg
//
LOCUS       DAJOTB010000104        16627 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJOTB010000104 DAJOTB010000000
VERSION     DAJOTB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.104"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..128)
                     /locus_tag="OHN28_003341"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="OHN28_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(270..485)
                     /locus_tag="OHN28_003342"
     CDS             complement(270..485)
                     /locus_tag="OHN28_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298678.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            complement(959..1561)
                     /locus_tag="OHN28_003343"
     CDS             complement(959..1561)
                     /locus_tag="OHN28_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298679.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(1655..1861)
                     /locus_tag="OHN28_003344"
     CDS             complement(1655..1861)
                     /locus_tag="OHN28_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCQ2298680.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGSFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(2057..3253)
                     /locus_tag="OHN28_003345"
     CDS             complement(2057..3253)
                     /locus_tag="OHN28_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCQ2298681.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEATRTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYSSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEAALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            4053..4193
                     /locus_tag="OHN28_003346"
     CDS             4053..4193
                     /locus_tag="OHN28_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HCQ2298682.1"
                     /translation="MNSHTRYKLTLAELEAFNSAVDNRLAELTANFTSACWLQSCHLS
                     VR"
     gene            4833..5402
                     /locus_tag="OHN28_003347"
     CDS             4833..5402
                     /locus_tag="OHN28_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2298683.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            5568..5969
                     /locus_tag="OHN28_003348"
     CDS             5568..5969
                     /locus_tag="OHN28_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298684.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            5957..6367
                     /locus_tag="OHN28_003349"
     CDS             5957..6367
                     /locus_tag="OHN28_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCQ2298685.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPSCGTGVYSREGGLQEGNWKDRN"
     gene            6651..8270
                     /locus_tag="OHN28_003350"
                     /pseudo
     CDS             6651..8270
                     /locus_tag="OHN28_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8350..8642
                     /locus_tag="OHN28_003351"
                     /pseudo
     CDS             8350..8642
                     /locus_tag="OHN28_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(8682..9716)
                     /locus_tag="OHN28_003352"
     CDS             complement(8682..9716)
                     /locus_tag="OHN28_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HCQ2298686.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(9719..10684)
                     /locus_tag="OHN28_003353"
     CDS             complement(9719..10684)
                     /locus_tag="OHN28_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2298687.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(10738..11496)
                     /locus_tag="OHN28_003354"
     CDS             complement(10738..11496)
                     /locus_tag="OHN28_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCQ2298688.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(11510..12724)
                     /locus_tag="OHN28_003355"
     CDS             complement(11510..12724)
                     /locus_tag="OHN28_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCQ2298689.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(12920..13258)
                     /locus_tag="OHN28_003356"
                     /pseudo
     CDS             complement(12920..13258)
                     /locus_tag="OHN28_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13726..14499)
                     /locus_tag="OHN28_003357"
     CDS             complement(13726..14499)
                     /locus_tag="OHN28_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCQ2298690.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(14501..15025)
                     /locus_tag="OHN28_003358"
     CDS             complement(14501..15025)
                     /locus_tag="OHN28_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2298691.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPS
                     GTYSLRQELIRTGD"
     gene            complement(15060..16256)
                     /locus_tag="OHN28_003359"
     CDS             complement(15060..16256)
                     /locus_tag="OHN28_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCQ2298692.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(16270..>16627)
                     /locus_tag="OHN28_003360"
     CDS             complement(16270..>16627)
                     /locus_tag="OHN28_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCQ2298693.1"
                     /translation="IENETPQDVEITRVYTMNNTDRHQDHIAVYQASMVACRYIPQIL
                     GYETPSTCLSFLPQVFESVDEVHFNKKILALQKHKSQIHRRYIQPEYIIAQAKFRGQQ
                     VSHSMCEGFVVHKMVL"
BASE COUNT         4309 a         3947 c         4152 g         4219 t
ORIGIN      
        1 attgccttaa tctccgcaaa gataacctcg tcattccgga cattctctgc taggcatatg
       61 tcgatgtaat ccataaacga tttcagctta acccttttgt ggccggtctt tctggctggc
      121 ggttcagggg atgttttctg gttatcgtgt accacaaaac tgggatattg ccccgatagt
      181 cacatttcag attgatcgcg gtctgaagat gtggcactga ggtaacgtgc tggtgtcact
      241 ggtattgctg aagttatggt ttttcggggt caggtgagcc tgttgtgtgt atgctttttg
      301 ttcagatcag aggggggctg tgcggacagc ggcttttgtc tccgggcatt cacgcctgaa
      361 cgatgaatgc gattttcagc aatatttgtc tttgtgacac tctctggttg agaagctaat
      421 gcgcgcccct gtttccacag agaatgaggc ttgccatcag ccgaaacatc agtattccac
      481 gccatacaga cggcgcctga ccatgtgctg atgcaggtaa aatgtttcaa ctacaactgt
      541 tcgggttgct atgcaacgaa acagcgattc tcacgatctt taactgcaac gtttttccgc
      601 ctgaaagctg actgcctcct gctgaggtgg gaggctatga ttcagtgcgt ttatagtctc
      661 cggggtatgc atgtaccgga gcataccatt tctggtggat aacaagccgg cacattcagc
      721 ctgcagtgaa gatggaggca cctggttgtg aagaacgttt ctgcccgtgg ctgcttcgca
      781 tcatcaatgg tattcatacc cgctcagaaa tgaccgggtg ctgaagccct ccgttatcgc
      841 gcagtataat gacgtatatg atctgtggac gagtgagcgg cgtgcctgat gtacgccgct
      901 attactgtat tttaccacag ggctggtttt ctgctgccag ccctgtggtt atcagacatc
      961 aactgtgagc ggcatcaacc gatgtacggt atgccataag cacgtactga tccatcagat
     1021 ttgtcagtgg ttcactctga ccctgacgtg caatgatcca ttgctccaga tctttttctg
     1081 atttgtgttt caggtttttc gtccagtcgt caaggctgtt tccggtcatt aagtcaagcc
     1141 agcagagcca gaccagtttg agacgtaact ccgtgccgcc aaactgacgg aagaggctga
     1201 tcagtttgtt tgccagagaa tgttgcttca ggcgctcaca ttcagacagc aggatccctt
     1261 ccagcatcgg atgatgaacc agcaggtgtt cgctggtgca taaattcacc accagatcat
     1321 catccaggga cggggagctc cgtttgtccg gaccataaat cagccggtgc agactctggg
     1381 gaacgatggc aatctccggt ttttcgccat tgaatgacac ccacccgggc gtcagattat
     1441 ggaaatagta gatattcgga tgacacacgc cccatgatac ggcgtgccgg gtgaagccgg
     1501 tcagccattt ctggtagttt tcatggagca gctcgtaacg ggtctgatac tgttgtttca
     1561 ttttcatatt cctcacgggg gataacaggc agcagagcgc cgccggcaga ccggagattg
     1621 cgtctgcttt gggcgaaggg ttagtggtta aaaattacgg acgggactgt gcaatacgcg
     1681 cctgcagcca ggcttccact tcacttttca gccagcggga gctgcgcccc attttgatgg
     1741 gggcaggaaa gctcccaact ttgatgagtt tgtaaaacca cttatcggtt aagccggtca
     1801 gttgagtgat aaacgccatg tcgaccatct ggtcatccat cagcgaaact ggggtagcca
     1861 tgagaatgtt cctccggatg ttgttaaacc agccggtgtg gtcatgaaaa cagcgcgcca
     1921 ccttcaccgg cataacaacc ggggagaaac gtaaaaaata atcgggaatc tggctgtccg
     1981 gagataaacg gggggattgc gatggcagag ccgtagcgga atcccggggg gtgcccctat
     2041 taagttgtca agcatgttat gacgcctgcg gggtataaaa agtcccgtcg cgcatcatgg
     2101 cgaacagaac gtcgcagcgt cgtctcgcca gggcgataag cgcctgattg tgtcgttttc
     2161 cctgactcat tttgcgggtg tagtaagccc tggagagcgg atccctgagc gcggcgaagg
     2221 ccgacaggaa caacgcccgt ttgagagctt tattaccccg tcgcgaggga tgctcaccgc
     2281 gtattgacga gccggatcgc cgagttaccg gcgcaaggcc agcataagca gcgagatgtg
     2341 cgacagaggc gaaggcgcgg caggcgacct cggtgaggag tctggctgcg gtcctgacac
     2401 cgactccggg catactggtc aggaccgggt aaagagggtg agcaagaact cgctgttcta
     2461 ccgcaagcgc cgcctcgtct ctttgcttac gcagcgtgat gagctggagt gccagacgtg
     2521 gcagtactac ggcagcggca ttcgtgccgg gaacgacgac ggtttgttcg gccagtgcct
     2581 gagctatgtc tgctgcaagg cgtttaccca gacgaggcgc aagtttgcag agctgggctg
     2641 ccagcttctt ctcacccagc gaagcgagtt tttctggtga ggaatatcgc tggagaagat
     2701 cgagtaccgc cgggtgatca agtctcggac cgagaacgcg ctccagtgcc ggatgtatct
     2761 gggtcagaag gccgcggata cggttgctgg cctgcgttgt ctgtgcggca agatcatcat
     2821 cgaagccgca gagcatggag agttcggcga tttgctcgtc agccagtttc agcgtgcgta
     2881 gcgcgtgagg cagggtacgg gtagcttcgg caatgatggc agcgtcacga gcatcagttt
     2941 tagcttcacc ggcgtgtaag tcggctatgc ggcgcatggc cagtccaggg aggtatccga
     3001 caaggactcc ttctgagcgg gcaacggcga caggtaacgc accgatggta gctggctgat
     3061 caacaaccag cagtatctga ccatgttgtt tcaggtcaga tattagcgac ctgagtttgt
     3121 tttcgtcgtt gggtaatgct ttatcgaaca ggcgtttacc tgaacgatta atggctacag
     3181 cgtgatgcgt atctttaccg acatcaacgc cgataaagac ctggacggat tcgtaatcgc
     3241 tggattcggt cattctgtct cccttgtata tgggttaacc agataaccac ggggagcagg
     3301 taccggcatc cacgttacag acggtcccgg caaaagtgcc tgacctgacc cctattagcg
     3361 gttaccagcg ccccaccaga cccggtgaca tcaccccccg gatcatggac gactgggggc
     3421 agtaatcatg ccgggtctgg ctggctaaca ccccattata aggggtacga ataaagtaac
     3481 gggggtgcga aaagatgctt ccgcacgcac gcgcgataaa gaggggatag ttttcgtgag
     3541 gacagggaga gaagcaggcg ttctgataat tgcagagttg ttttcggagg tggttacagt
     3601 gagtcagccc gttcctggcg agcaggagac tgatatcacc gatgggataa ggttgtctga
     3661 ttggtattat aaatataaga agtcatcaga gagtaacgta aaggatttgc cagaataacc
     3721 taagagagta aatctctccg gtaacagagc gctgtgaagc attctgtatc agagtgcaaa
     3781 tacatgaacg ctgatgcgta aaatcagcag tgacacgcca gctcccccta tgcccattcc
     3841 tgtataagac atgaactgag cgtattaatg ctcctgccct gagactgagc cggtgagtga
     3901 ggggctgaga aatccctctt gacactgctc taaccagaaa tacttccccg gttgttgcgg
     3961 tgcgtccgga tccccgataa cggaacacta aggctgtatt gcgggacacc aggtaagtgg
     4021 cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc ccacacgcgt tataaactta
     4081 cccttgcgga gctggaagcc tttaactctg ccgttgacaa ccggctggca gaactgaccg
     4141 caaattttac gagtgcgtgc tggcttcagt cttgtcacct gagcgttcgg taacggaggc
     4201 gcgggcggta atagactgat tttcaggtat taccggctta cggcgtgtgg ttgatattga
     4261 ggggctgtga gtttcgcgta tttgtcagaa acccagacga gttttcctgt atgggacgaa
     4321 ataaggcagc ggcaatacct ggaaaaattt aatatggtat gaaggagaac ctgtcggtta
     4381 cgcttaatat acccggtaca ctgtcagctc aggggataca tgaaccgtta tctcaccagc
     4441 tacacagtat atacacactg acgctgacag tagtcagcag gaccacaata atcagtgcca
     4501 gcagtatggt aacgatacgg tctgtattcc ggtaatagcc gaaggggtcg acagaaccac
     4561 aatacgggca acacactgta ttatgagtaa tcgcgtttcc acagtccctg cacttctttt
     4621 tgcgtatcac catcactccc tcctcacagc cttacccgta accggttttt tacattaaaa
     4681 aaacaacgct gaaaattcaa ccgtcagtcc ggagacgacc gttcgggtta tcacagagtg
     4741 cctgagacag tgccctgccg gaggtcactt cagggactct gcgcattttt tacgacgtgg
     4801 ctattccgta tgaaccatac ggagatttaa ccatgaccta caaatacaac cccttctggc
     4861 agcaacgtat tcgtgagaca gtgcggcacg cactgaatgt tcatccccgc ctgacggcat
     4921 tgcgggttga cctgcgtttc ccggatgtac cggcagcaac ggacgcagct gtgatatccc
     4981 gcttcatcaa tgccctgaaa gcccgaatcg acgcttacca gaaacgtaag catcgggaag
     5041 gtaaacgcgt gcatcccacc actctgcatt acgtctgggc ccgggagttt ggggagtgca
     5101 aaggtaaaaa acactatcat ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg
     5161 gtgattaccg cgctccggga tcactggccg gaatgattga acaggcctgg tgcagtgccc
     5221 tgggggtgga tgccggatgc catgccacgc tggtgcattt tccggcctgg ccggcggtgt
     5281 ggctggcgcg taatgatgac accggctttc agcaagtgct ggaacgtgct gactatctgg
     5341 cgaaggagca taccaaagct cactgcaccg gtgagcgcaa ctttggctgc agtcgtggct
     5401 gagccagaca gacgagttat ctgacttcag tgtgatgacg tgttcatggc caccacactg
     5461 attttccctt atgagactga actgaaaccg ccattcacac ctgaccacgc tgcccgtacc
     5521 gggacggctg ttgcctgcgt gtcgtctgac ggtaaggaac atatactatg tacgcaaaat
     5581 cctttctcgc tcttgatagc aacggacgtc tgacgggcgc ccgtactgta caggccgcac
     5641 cttatgctca ctacacctgc cacttgtgtg gcagtgcact cagataccat ccgcaatacg
     5701 acactgaact tccctggttt gaacacactg acgacaggct gacagagcac ggtcaacagt
     5761 gcccttatgt caggccggag cgcagagaaa tacagttgat taaacgtctg cagcaattcg
     5821 taccggatgc cttacccgtg gtgcgtaaag ccagctggca ctgcagacaa tgtcaccacg
     5881 attattatgg ggagcggtac tgcacacact gccagaccgg aggattcagt attccccgga
     5941 caactcagga ggaaatatgc gaattctgaa ctgttatatg gcgaatgaca gcaaaggcca
     6001 ttttgttacg gcgaaagaag cagcgaagca caaccgccag gacgttttgt gctgtgtgtc
     6061 ctgtggatgc ccgttaacac ttcagcgggg caatgacgga caaccaccgt ggtttgaaca
     6121 tgaccagatg actgtcgctg agaaaatcct gctgcgatgc acctggcttg acccggcaga
     6181 gaaagaggcc cgtcgtttgc atctgcaggg tatgacggtt ccggattata cggtgaaggt
     6241 gagaaagtgg ttttgtgtga tgtgtgacga agattatgaa ggagaaaagt gctgcccgag
     6301 ttgtggtacc ggggtataca gcagggaggg ggggctgcag gaaggcaact ggaaggatag
     6361 gaactgagcg gttcagtcgc ggaaatcagt tgtagagctg taatgatatc tccgggagtg
     6421 ctgcgttggt aggggcatcc cgggatagag aaattttagc actttgccct gaatgattgt
     6481 agacgtcccg ggtactcgta ccggtctgag ttattacagt tagatattca ctttttctcc
     6541 cgcatacttc acatctgtca acgacggatg aaaagtggtt cttccgcaac ctgggtggaa
     6601 gaaaatggat gaaactttct ggcgaacaag gatctaagct gttttaagtt atgggcaacg
     6661 caatgcactc tcttaagaca cttctacagt taccttgcgg atggcgatgc agtcgacaaa
     6721 ttattagctc tgacggtata accctccatc tccacggaaa acgcaaaaca gcacaatgtc
     6781 ctgaatgctt taagcgtagc gactctgttc atagttgtcg tcggcgccgg atacagcatc
     6841 taccctgctc agggcagacg ctatggcttg tattttccgt ccgccactgg tactgccgta
     6901 acccggcttg ttaacgtaaa atttttgccg agtcgcttgc tcccttcgcc ggttcacacc
     6961 agcagtcttc acaggcgtta caaaatttac aacgtcaact gggattaata gctggaggtg
     7021 aggctggaaa acgggctgca acggcagcgg gtctccgttg cagtgctgat actcttcttc
     7081 gcagggttat caataccccg gagacgaaac agtcaggcgc gcctcatgtc ggtattgatg
     7141 agtgggcgtg gcatcggggc cactgctgcg gtatgttaat agtcaatctt gatactcacc
     7201 gtcccctcgt cctgcttccc ggccgtgatc agcgtacgct ggcgacctgg ttcagaaaat
     7261 atccggaaat acaggttgtc tcgcgtgatc gcagtggagt ctatgcgaca gcagcacgtg
     7321 aaggtgcacc tcaggccaga caggtggccg atcgatggca cctgctaaaa agtattggtg
     7381 atgagcctga acgaatgatg tacagacata tgcctctgat acgtcttgtt gtcagagagt
     7441 tatcactgaa gaaatcacct gagccagaaa tatctgtgcc tgtagcatcg ctccgtcgtc
     7501 cggaacgcct taaacagcaa acccgcaaaa aacggcatca gcattggaca gaggttatgg
     7561 ccctgcataa caagggatgt agtttcaggg aaatatcccg tattacaggc ctgtcgcgag
     7621 tgacagtcag tcgctgggtg cgttcaggaa cattccctga aatgtcaacc cggcctccaa
     7681 agcgagggct tctggaccca tggagggagt ggttaaaaga gcaacgagaa agtggtaatt
     7741 ataatgccag ccggatatgg cgggaaatgg tggcccaggg ggggacaggc agtgaaacca
     7801 tcgtcaggga tactgttgcc aaatggcgta aaggctggaa cccaccggtt actactgccg
     7861 caagacttcc ttcagtgtcc cgggtaagcc gctggttgat gccctggaga ataatcaggg
     7921 gcgaagaaaa ttatgcatcc cgatttatta gtctgatgtg tgaaaaagaa ccggagttga
     7981 aaatagcaca gcaactggta ctcgagttct accgtattct gaaaacctaa aataaatcac
     8041 agctaagcag ctggttcact cgagttcacg aaagcggctc agcagaactt cggtgcgtag
     8101 ctgcggggat ggaagctgat gctgcggcta tatgtgaggc aatcagcagt cgctggagta
     8161 atggtgttgt cgaaggtcat gtaaatcgcc tgaaggtgct gatacgccag atgtatggtc
     8221 gagccggatt tgaactactt aggcggaggg taatgagtcc actggcatga aaatcttcac
     8281 ctaggttgcg gaagagccac ttttgcactg ttcgtgacag tatggccttt atgtactcac
     8341 tttgttaccg gtagctgaat catcactacc gtgctgcaaa tggtggtgaa cagcatcaat
     8401 tgtggaatga atgttgccga agcgaccaat gcgcctcgtt tccaccatca gtggttgccg
     8461 gactagctgc gtgttgaaaa gggtttagtc cggatacgct caagctgctg gaagcaaaag
     8521 gtcagaaagt ggcgctgaat gaggcgatgg gcagcacgca aagcattatg gttgggtcgg
     8581 atggtgagtt gtacggcgca tccgactcgc gcttggtgga tgatttaacg gcggggtatt
     8641 aattgacatc ccccggcgca gtgtgccggg ggataatggt tttatgcggt caataatcgt
     8701 ccgggattat cgatacagag ttttttgagg atcgttttat cgacgccgcg ctccgccaga
     8761 taggccagaa aaacatctgg aacaaatccc cagccattac cgccattttt agcccacatt
     8821 tgtttaagga agacatcgtg gctgagaaca agctgatcgg cgtatcccaa ctcaatgaga
     8881 tgagcgacgg catcggcagt ctcctgcacc cctggcgcta ttccctcttt cgggaaggta
     8941 atgtctaggc caatcatgtc gaattccagc cagacaccct tatcaagcat tttccgctga
     9001 tacgccacgt cttttcctga cggatccgag tgtgcgagag agactttatt tggcgagacg
     9061 cccatttcac ctaacacaat gtcgagtact tcatcaccgc gacgaagcca gcccggcatg
     9121 tgaatattca tcgccacatg aggattgtta atctgtgcca gcgaggcagc ccgcaagctg
     9181 ttatgctcgg cttcggtaaa tgtcggtgag acacctattt caccgatcat tccggcacga
     9241 atatccgtat cgccaatccc ttgattcaat tctttatcga tcgttgccgc cagttcatca
     9301 accgttttat gaattctctg gctttcaaat ttttccaggt agggaccgga ggaagcaaca
     9361 atattcagac ctgtttttaa tgcgacttct cgtaatgcct gcgcatctcg tccgatagac
     9421 tcagaccctg tggcatcaac aatggtgcga ccaccgagcg agataaagtt attaatttcg
     9481 aagatcacat cttcaatagg tttgcggtcc atattatcag cgcagcaata aggatcgtgt
     9541 tttaatgccc actggatttc tgccgtgact tttttatcaa caagccgttg tgaaaaagga
     9601 tagcaaggag catccacaac agaggaaaga tcgttgaaca gatgttcatg aggaagtgtt
     9661 agccccatat cttcacgggc gacggggccc gtcacagttt gtagataatc tttcatgatt
     9721 atacctccat tttcttcatt gttttcgagg ttctgtattc cggaatagtg gtaacaattg
     9781 caccgaccag tgcaaacagt gttccgatga tcgtgaccag ataaacggta ttgcccagcg
     9841 atggaattaa tacgtcaatc aatactgatc ccagtaactg gcccgccgtt gaagcaaccc
     9901 ccagcatcag taaccccaga cctctgacca aaatggccat taaaccgata gacattagtc
     9961 caagcgggcc gccaagatac atccaccaga catctggtaa ttgaacagtg acgtgcccta
    10021 atgcgacacg aatagctagc gctgtgccta aaacacaaaa tccaacgatg aaattccagg
    10081 tgattgaaac cagcatggat cccgtagctt cagctacttt tgcatttcca gcgggctgcc
    10141 atcctgcaag caaaccagct aagaacggaa gaatggcaag taagatgaaa gaggtagaat
    10201 gccactgcgg tgacacaaca aaaatcgtag caataaccgc aaataatgca ccgatgatgc
    10261 gataaggggt aaagtatttt ttctcgtcga caccgatacc aaagcgatcg caaagtaaac
    10321 cagacagcaa tagcgcggag atcagtgcgg tttgaaacgt tgcgaccccg agtgcgctgg
    10381 ctgatgcgcc ttcagaaaat accaccatcg ctccacagag accggcaaac cagttccaga
    10441 gaggaacttt tcggttttta atcagagaag gaattgccgc aaattgttgc cgggtttctt
    10501 ttcgcgccat aataataaaa aacatcacga ctaaaccact ggcaaaggag ataactgcgc
    10561 atgcattacc atcctgtaac cagtgaccta attgaccatt caccgctgat tgcatagggg
    10621 agagcatacc cgcaagaatc gtggcaagca tccataaggg agttgagtac ttattctttt
    10681 tcatacatca atccttctat tatagtaaat ggggtaactg gagttacccc attattatta
    10741 tcttgcaata tgagtcagta tatttaccca caacgcggta tagaacggcc aggacataaa
    10801 ctgctgtgta ccccagtgcg gtgaacagtc actcataaag caggctattt tcccgttgtg
    10861 atagttacca aagaccagta atggatcgtt gtttattgtt aataccacct cagcattttc
    10921 tttagcgatc gcccggttat aacctaggaa aaaaggatat tcagagaacc ctttaataac
    10981 tggatgttca ggttgtgatg gctgtgcaat aacaccctct ggtttttcga cacgatcgtc
    11041 accatctaac atcgtaaccg ggagcacatc agccagaact gtatttttat aattggcttt
    11101 tgcttcaatt cccatgaatg agagatagcc gccaatcatc aataacccac caccgttatt
    11161 cacatattct ttaatcagtt cgagagcgtt tggttttatg cgaagttgat aaaaagtatc
    11221 attttgtagc aggaaagtat tactaccaat atcactaata acgatggcat catattgttc
    11281 taactgtgcc acatcttcag ggaaggcaat ttgtaccgta tgtgccggca tataggtgac
    11341 atctacctga ctatttttca gacattggag taaccaggtc gcaccttctt catatttact
    11401 tgaggtaaaa ctgtcatatc cttttgaatg aatcatatga atatgccagg attccccaat
    11461 aaagagcact tttagtgatt ttttggtgtt attcattctc tgtcccttct taatgcgtaa
    11521 aaatagtttg ctgataatgt atactctgaa ttcgatgtat cacagattca tgttcaggca
    11581 tgtcggaagc attgcttgtc tccaccgcta gggatgcaaa ggcgctggca taacacagtg
    11641 ctgattccag tgattttcct ttcgcaagtc ctgatgccag agcgccgtta aaagcatccc
    11701 cggctcctgc cgtattttta acgaccgctg gaaacgctgg cgaatgaatg aattttttgc
    11761 catcaaatgc gagcgagccc ttgctaccga gcgtgataac cgtgtttttt acccctttat
    11821 tgtggatagc ttctgcagct cgtttagctg attcaagatc gtgtacgtct atgccggaca
    11881 gtaaaccagc ttctgtttca ttgggggtga tataatcaac atcctgaata agttcgttga
    11941 cgatatcatt atagggtgcc gggttaataa tgaccgggat actgtttttt tgcgccaatg
    12001 tgatcgcttg ttgcagagcg gtataatttg tttcgagctg cagaagtatg acatcagaat
    12061 taataatcgc ttctttctga atagttattt catcggacga cattgtcatg ttggcaccgg
    12121 gatagatcgc aataacatta tcgccagtac cttcattaac aaggatcgtg gcagttccgg
    12181 tttgcgtttc tttcgtttga taaattatac ttttataaat cttagacgaa ttcataaagt
    12241 tgatcgcata atcactaaag tgatctgtac ctacttttgt tatgaaataa acatcagtat
    12301 cggcaaagct tgctgccagc gcttggttac agccttttcc ccccggtgaa aatataaaat
    12361 tacttgccag aagcgattct ccgatagtgg gaagcctcgg caaataactg atgatatcaa
    12421 cattaaacga accaataacg catacttgac ttttcatagt tgagtggcct ttttttattc
    12481 cctgggctgt atcataattt tcaagcgcat aagcgatttc atttttcgca atagtatcaa
    12541 gcgagtctag gacgataaaa gcaccgccat gacatcgagt cagatagcca agcctggcaa
    12601 gttcattaag atctgatcga atagtttctt tcgttacatt gaggctattg gacagatcgg
    12661 atacttttac agaaccgaat ttactgagtc gtttcaatat ttcatgatgg cgttcaaatt
    12721 tcatgcttca cctcacttca gttacgttaa tatctatcga tgtatattga tgtgataatc
    12781 accataaagt ggcggggaaa agcaaaaaaa agcaaaaata gcctgagaaa aggtaagcgt
    12841 taatggagaa tcatattgat gcttatttat tggtgagaac tacgttccat ggcaggagtt
    12901 catcaatacg gttggaaggt tattccggca gtacgctcag gatatggcgc agatacgctt
    12961 ccggatcaat gtcgttcaga aggcaagtgc cgataagccc atacaacagt gcactgcgct
    13021 cgccgccgaa gaacataaag tttttatttc cgagacagat ggcacaaagg gctttttccg
    13081 cggcattatg tctgcctccg cctgaccgtc aacactgtaa taacagagtg cgccccactg
    13141 attcagtaca cagctgaacc attcacccag tctggatttt tggatagcgt ggcatttttc
    13201 tccaccatgc atgcagtgag tccagtaaca gtttgctctt cttctgcctg accacaagat
    13261 gtgctggact ggtaaacgat ggggatacct gcttacgcga gagcaataag tggctggcca
    13321 caattctcgg tacaaaactc tgctgaatag cagcgatttt cccagcgtgg tgcgtcgttt
    13381 gcattttact ggttctaagt taacaattat ctgttcacta atgactactc taaaaacttg
    13441 tatcaggact atcacttatt taagtgatat tggttgtctg gagattcagg gggcctgtct
    13501 agagagatat aaagatacct tcaccccggt gatgataaaa taaaagtatc tcagctatta
    13561 aactgatgta cgattcgggc aataacttat tcgtatagag caccttttct ctggtgctct
    13621 atacgaataa gttattgccc gaaattaatt atattgttta ttatcaaacc gagttatagc
    13681 ttttctctga aaactaccga ataaaatata ctgatagact gctgcctaga atttccagat
    13741 caggtgggct gcaagttgca gaccgttata atcatcggtt ggggtgttga accacacttt
    13801 acctgccgtc aacccgagat taagttgctc agtcagcggt tgaattcgtt gcaaactgat
    13861 gctcttcatg ctgccgtagc gtgtttctgg cgtccagtcg taggtgtaag cacctgttcc
    13921 gcggcttagc catagttgcg tataaccagc accgcgcggg tccttcagac gcacggaaat
    13981 catattgcta taactactac ctgcatcgct ggagtcataa tgggtatagc ggtaactggt
    14041 gatcaccggg ccagtataga gtgatacgcc gccttgccag gcattgactt cgacatcatc
    14101 gtaatattta gtgtagcgat aaccggtcgt gaaaagcgtt tttggcaaca gggctaggtt
    14161 aatatcctgt cgaaaatcct ggcgggcaaa gaccggcgta ttatccgcaa aggcaatacc
    14221 cgttcgggtt gtcagccagt tattccattt gtaccagact gcaccctggc cgcgagtggc
    14281 attgaaatgt tcagtttcat aatcacgacg cccctgggaa agattaaaaa gcagcgttgc
    14341 gttttcaact tttgctacca gttcagcata cgctaaatta cggttgccat gttcgccaga
    14401 ataatcggta aaatcgtagc ccgcggtaat ggaagttaac ccggcataag cgggcaatgc
    14461 actaaaaaaa aacggcacta ataacgtatg ctttatcatt ttaatctcct gtacggataa
    14521 gttcttgtcg gagtgagtaa gttccactag gtaaattacg ttctacaatc acgcgcgggc
    14581 caagttgtgt gttcggagaa ataattcgcc caggcaaaat aataacttgt acgccaaggc
    14641 gtgaacgctt cccgatataa caacctaatt tatcgcatcc ggtagcgata attccctctg
    14701 gagttcgaac agacacgggt tgttcatcca gacgatgatt actggtacgt acttgtgcgc
    14761 ccaaatatgc ctggtttgca actaccgagt cggcaataaa gcattgcgga ccaatcgttg
    14821 cttccgcttc aataaccgca tttttaattt cggtggcaaa accaattttt acgccattgc
    14881 tgattattgt gccaggacga ataaacgcat aattacctat caggcagttt gcgccaatca
    14941 ctaccggccc ctgaataacg gcaccgtggc aaatacgggt attcgcgccg ataacaacct
    15001 caccggcact ttcatcaata atcacctggt ctgcaatctg aacatttttc tctttagcat
    15061 taagcttcag atagtttttc agttcctgtg acagaaggtt gggaataagc ggttgacgct
    15121 tccaccagtg cggaatagcg ggtgggtttg tcgcgctgag gttcgcgtcc agtaaaaaag
    15181 gcaataagtc cataaataat ccagatggca taagctaata atacatacac aacggaaagc
    15241 ggtgctaaag gcaccaacaa ccagcaacga tgaaaccagg cgctaaatgc accaataaca
    15301 caaactacgc cgacccagat aagcgggaac attgccaaca ccacaccatg tggcccggtg
    15361 gtgatgaatt cattaaacca ggtagtgaga taaataccaa cgccataaag cacaaccaac
    15421 agcataggaa atatactgaa gataccgaag cggctaaata aaagtctttt atgcaggcgc
    15481 atacagaccg cgtagcccac aatccaacgc cgccagcggc gccactcctc acgcggactg
    15541 ttacattcct gtgggtatac gatgcagcga ttcgcctgcc gaatacggta gccgtttgcc
    15601 accaacgtcc aggtgagatc aaggtcttcg actttagtac gatcagagaa accgaactta
    15661 cgcaatacat cagtacggaa catcccgcag gcaccgctga taataaacgg tgcaccgccc
    15721 agaaactgct gtagcgtgcg cttcataaca atcatcggca actttacggt ggcgcggata
    15781 tgtggcagca gacctgcccc ttttaacgcg gtggagggaa ttccgccaac ggcatcagcg
    15841 ccgcgctcaa tttctgccag catatagccc attccatcat tatcaggtgg aacgaaagta
    15901 tctgcatcac ttaaaaatac ttgatcacac gtggcgtatt ttagtccatg cattagtgcg
    15961 ccacctttac cggtgttttt ttgcgtgacg gcaacaaagc ggtcgcccca tttgcgtttg
    16021 acttccgcca tgaccgcttc ggtattgtcc gtggatccgt cgttaacgca aattacccgg
    16081 caaaaataag ggttacgcag cagattatcc agtgactgcg ccagacacgg gccttcgtta
    16141 taagctggta taatggcgtc aatacagcct ttcttttgac agggtttacg tcttagcgta
    16201 cttaaaaacc atagcaacat tgctatggtc atgaagataa ataacaaagt tttcatttct
    16261 attactccgt tacaacacca tcttatggac aacaaaacct tcacacatag aatgactaac
    16321 ctgttgtcct cggaatttgg cttgagcaat tatgtattct ggctgtatgt atcttcgatg
    16381 gatctggctt ttatgctttt gtaatgctaa tattttttta ttaaaatgga cctcatctac
    16441 agattcaaaa acttgaggta aaaatgacag acatgtactt ggtgtttcgt atcctagtat
    16501 ttgaggaata taacgacagg caaccattga tgcctgataa acagcaatgt gatcctgatg
    16561 cctgtcagta ttattcatgg tgtaaactcg agtaatttcc acatcctggg gcgtttcatt
    16621 ctcaatt
//
LOCUS       DAJOTB010000105        16608 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJOTB010000105 DAJOTB010000000
VERSION     DAJOTB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.105"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            225..806
                     /gene="mdaB"
                     /locus_tag="OHN28_003361"
     CDS             225..806
                     /gene="mdaB"
                     /locus_tag="OHN28_003361"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCQ2298694.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            837..1151
                     /locus_tag="OHN28_003362"
     CDS             837..1151
                     /locus_tag="OHN28_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCQ2298695.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1196..2083)
                     /locus_tag="OHN28_003363"
     CDS             complement(1196..2083)
                     /locus_tag="OHN28_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCQ2298696.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2080..3027)
                     /locus_tag="OHN28_003364"
     CDS             complement(2080..3027)
                     /locus_tag="OHN28_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2298697.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3038..4075)
                     /locus_tag="OHN28_003365"
     CDS             complement(3038..4075)
                     /locus_tag="OHN28_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2298698.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTTVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4084..5067)
                     /locus_tag="OHN28_003366"
     CDS             complement(4084..5067)
                     /locus_tag="OHN28_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2298699.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5064..5873)
                     /locus_tag="OHN28_003367"
     CDS             complement(5064..5873)
                     /locus_tag="OHN28_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298700.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            6247..8388
                     /locus_tag="OHN28_003368"
     CDS             6247..8388
                     /locus_tag="OHN28_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HCQ2298701.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(8452..10344)
                     /gene="parE"
                     /locus_tag="OHN28_003369"
     CDS             complement(8452..10344)
                     /gene="parE"
                     /locus_tag="OHN28_003369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCQ2298702.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10373..10954)
                     /gene="yqiA"
                     /locus_tag="OHN28_003370"
     CDS             complement(10373..10954)
                     /gene="yqiA"
                     /locus_tag="OHN28_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCQ2298703.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(10954..11781)
                     /gene="cpdA"
                     /locus_tag="OHN28_003371"
     CDS             complement(10954..11781)
                     /gene="cpdA"
                     /locus_tag="OHN28_003371"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCQ2298704.1"
                     /translation="MESLLTLPLAGEVRVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(11806..12228)
                     /locus_tag="OHN28_003372"
     CDS             complement(11806..12228)
                     /locus_tag="OHN28_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCQ2298705.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(12229..12858)
                     /gene="nudF"
                     /locus_tag="OHN28_003373"
     CDS             complement(12229..12858)
                     /gene="nudF"
                     /locus_tag="OHN28_003373"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCQ2298706.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            13063..14544
                     /gene="tolC"
                     /locus_tag="OHN28_003374"
     CDS             13063..14544
                     /gene="tolC"
                     /locus_tag="OHN28_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCQ2298707.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            14692..15363
                     /locus_tag="OHN28_003375"
     CDS             14692..15363
                     /locus_tag="OHN28_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCQ2298708.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            15369..16529
                     /locus_tag="OHN28_003376"
     CDS             15369..16529
                     /locus_tag="OHN28_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2298709.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         4402 a         4417 c         3984 g         3805 t
ORIGIN      
        1 gaaaaactcc tgtatcattt gcataataca tctctcctgt gggagcttat gggaagttga
       61 tttaagtatt gtcattttca gaaaatattt atcccgcatc ggtgaatcag agaaagatca
      121 gacttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg taccgctctg
      181 tgacgtacta tagtcggcaa acatctcacc ttgaggttaa aaaaatgagc aacatcctga
      241 ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg
      301 aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt ccgcattgtt cgcgccgaca
      361 gcgactacga tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc
      421 agatgcctgg ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat
      481 tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa gatccatcaa
      541 aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga
      601 acgcaccaat ggaagccttc accgaaaaag atcagttctt ccacggcgtt ggtgttgacg
      661 gtgtgtacct gccgttccat aaagcaaacc aattccttgg catggaaccg ctgccgacat
      721 ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca
      781 agcatcttgt ggaaattttt ggttaactag agctctggct ttagaaggag ttaaccatga
      841 ttacagtaat cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcagtgttgg
      901 atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc
      961 caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga
     1021 tgattgagca gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga
     1081 aagcatatag cgaagccgta aaaggtgacg tgctggagat gaatattcgt atcctgcagc
     1141 cggggctttg acatttctaa aaagaggccg gtgctgctac cggtctcagc ccagattagc
     1201 cattctttct tcccgttttc agtcctcgat ggaaatcgtt aattcgctta atggttttaa
     1261 ccgcacgctg cggcactgcg gaagctaccg accagacaat catttccaga cacaacaata
     1321 ccgtaccatg tagcggcgtt ttcccttttt catccccgcg cggaacatga atcacgatac
     1381 tggcgtcttt gctaaaacgt gaatccagcg cgtttgtcag caaaataaca gggatcccca
     1441 gccgcctcgc ctcacgaagc gttgtcagcc cttcccggtg cgcagatttt tgcgccatca
     1501 tgatcagaac atcgccacgt tgaagcgcga tcagttgctc ggcaaggcca atacccgtac
     1561 ggtttagtgc cgtggcaggc aaacctatgc ggttgaatag tcgtgcggta tagtccgcca
     1621 gaatacccga agcgccaatg ccaaaaattg cgacttgccg tgcctgcacc aacaacgcta
     1681 cagcttgcgc aagcgcgtgg cggttttcag gctctgacag gacattacag gtatacaaat
     1741 gcccttcaag aacaaagtcg attgcggtgt taacatcgct cgtcaagtta ctcaccgtcg
     1801 tagacatctt ttcgctggaa ctaagggctg gaccaagcca ctgttccaga gtgcgtttca
     1861 gatcacgcag cccggcaaac cccagggcct gtatagccct gataactgtg gcatcggaag
     1921 tgttcaacgt ggccgcaatt tccatcgccg tttgctcgag taccacttca cgatgctcat
     1981 taatgtagct ggcgacaact cttaacccag gcgttaactg actcgcgcga gtgcgaaaac
     2041 gctcaccaaa aatatcaacc cgcactttat ctttctggat catttagcct ccggtagagg
     2101 tcgccctgca atttgtgatt gtatctgcgc agccatcagc cccagagatg caaaggagtt
     2161 agaaattgcc tcttcacgag aaatcagcac ataacgcccg gaacgacagg cagtcagaaa
     2221 ctgacaccat cccggcatga ctttttccat tgctgccagc tcatcctgag gtttaccacc
     2281 ggtgtcaccg cgccaggttg caaaaacaaa atcggcatcc agttcaggta aacgctccgc
     2341 gctcacatcc attcgcccac cttcaggaat gctctcaatc agcggcggaa aacgaaaacc
     2401 agcatcgcgt aatacccgtc ccagcgaatg atagctatgc atcacattaa tttttccctg
     2461 gtttgcctga atcaccgata ccgttatttt ttggctgtcc agcgtcgcct tcagcgcatt
     2521 gatttgtgcc tgatagcggc gttcaagtat cgccagctgc gactgcgttc ctgtaagttc
     2581 tgccagcttg cgatagattt ctggcgcgcc ccctttgaga tgatcaatgc tcaccgtggg
     2641 cgcaattttt tcaagttgtt caatgggcgt attacgggtc ggttctgtga tgatcaagtc
     2701 aggctttgcc gccacaatcg cttcaatatc aatatcagcg gtaccaataa aggcgatcga
     2761 tgaattatca aagtcgacac ctgtcaataa cgcgccagat cgaatgaaat ggctaccgtc
     2821 aggccgagtt cgcccgtggc tggcgacagg aggtacaccg agctcaatca gaggaatagt
     2881 gatatcaaga tcgtgtaaag agacaattct ctttggatga gcaggaacag tgactttgcg
     2941 ccctaaatca tcagtaaata cctgaacagg ttctgttgca cgggaaaaaa atgacaacag
     3001 tatcagcaga gaaaaaaata aacgcatata actcccgtta aaaactgttc ctgcgctgcc
     3061 agagcagtag caaaaaaaat ggtccaccaa tcagtgagat aatgattccg gcaggcagtt
     3121 gcaaaggcaa aaaagccaaa cgaccaatgc tatccgccag aataactaac aacgcccccg
     3181 ataccgcgct ccccagcagt aatgtcgtct gcccgccacg tagaataaaa cgtgatatat
     3241 ggggtgctat caatccgaca aaaccgatgt taccaacaca cgaaacgcag acggttgtca
     3301 ggattatggg tgcggcgaca cgtaacaacg ccagacgaga actattcacc cctaacccgg
     3361 ttgcaacctg atgaccaagc agagcgatat ccgcagtcct ggcggtgaac agcaataaaa
     3421 gagcggctgg caacatccaa caggctgaaa taccgactaa catccagtta gctgcatgca
     3481 ggcttcccga taaccacatc aatgcagttt gtacatctcg aacatctgca gtggtcataa
     3541 atacaccgat accggcggcg aaaaaccagg aaacaccaat accaatcaga acaaatcgcg
     3601 ggcgggaaat atcgcgcgca caaaaaatca cgattaaagc caccaacagg ccaccagcaa
     3661 gaccagccac tggacgccag aacattccca gcattggaaa ctggaaaatc agccacagca
     3721 cagccacgct acatccttct ttgacgccga ttaaaccagg atcggcaagg ccgttacgtg
     3781 caatggattg cattgcggcc cctgccatac ctaacatggc cccgcatagc aatgccatga
     3841 tgactctcgg caagcgaata tcctgcacaa tataacgtgc gtctgtgctt acgttttctg
     3901 gagcaaacaa cgctcgtcct acttccgatg ccggaacaga gaaagaaccc tgcatcagac
     3961 cgaatatcag tattccaacc gcaagcagga acagcccacc aagcaacagc agatttttag
     4021 ggcgcaccag tgtcgaaaac ttaccaacac gtagtgggcg tagcccgacg ttcattatcc
     4081 gactcatttg aacatcctcg tcgccataat gacaaaaaca ggcgcaccaa ctaacgccgt
     4141 catgacaccg gtcgctaact catggggcgt gaaaagcgtc cgggcaataa tatctgccag
     4201 taataacaaa agcgcgccga cacaagctga aagagggagc agaacgcgca gatccgctga
     4261 taccagacgc cggacaatct gcggtactag caggccgaca aaaccaatgg ggccagcaat
     4321 cgatacggct gcgccacaga gcaacgcgat cgcaagcagg gtaaatgttc gcgtgcggag
     4381 tacagagacg cccagcccct gcgccatgcg atcgcccagt gccaacatgt taagggaggg
     4441 tgcaaggtaa agggctaaca caaatccccc cagagaaaac caggcggacg tacccagcgt
     4501 cgcccaatcc tgccccgcta aatcacctgc tagccatgtc cgcatcgcca gcagtgtttg
     4561 ttcgtcaaga atcaggattg cagccgttat tgaggacacg aacgccgata gcgccacgcc
     4621 gcataaagta actttcattg gcgttacccc tgaacgccct gcggaggaaa gcagcaatat
     4681 caacaggaac aataacgcgc cccccgtaga tgccagcagt gggcgtccaa ccggaaaggc
     4741 cagtccaagc gcactggcgg caacgacagc aagagcggct ccggcattta agcctaaaat
     4801 atgtggctcc cccaatgggt tacgaatgac agcctgcaac aaagcaccag caacaccaag
     4861 agaagcaccg gtaagcaatg ctgccgctaa acgcggcaaa cggagtctta caattacgtt
     4921 gtgatcgaag ttttgcggat caaaatgaag aaatgcctgc aacaccgttt gtggtgcaat
     4981 aaatcgggca ccaatccctg gatataccac gcatcccacc agcagtaagg cgaggaaaat
     5041 tacagtaacc agccctgtgc gcatcatact ttttctacac cacgaaacgg catgaagaac
     5101 ggctttcccg tcagaggatt tattgatgcg tgaacatcaa cgtcaaagac cgcttttacc
     5161 agttcaggcg tgcaatgctc cccctcattc aacacacgca ccactttccc ctggcgtaaa
     5221 aatatcaagg tatcaccata attgacggca aaattcagat catgcaacac cacgacgact
     5281 gtacgcccgt gatgacgcgt taaatcatgt aataactcaa gaatttcaac ctgataacgt
     5341 aaatcaagcc aggttgtggg ttcatccagc aggatataag gcgtcttttg cgccaacacc
     5401 atcgcaatcc agcaacgctg gcgctgccca ccagagagtt tttcaacagg taaatgtgca
     5461 aactcctgcg taccggttaa tttcagggcc tcttccaccg cctgctcatc ggcgtcgctc
     5521 cattgacgta tgaaattttg ccagggaaaa cgcccacgcg acaccagttc ataaacggtt
     5581 aagccctccg gcaataatgg cgactgcggc aagatcccaa gtctacggga taacgctttc
     5641 gttggttgct cgtgaatcgc tttgccatca agcaatatcg aaccgcccat gggttgcaag
     5701 atacgtgcaa tggtgctgag caacgtcgac tttccacagc cgtttgcccc aaccagaacg
     5761 gtcatttttt ctgtgggaac ggagaaagaa acaccatcaa caatgatctt cttgtgatac
     5821 ccggcagaaa cattgtcgag gatcagcccc tgcccttttg cgttatgtgc cacgtgtacg
     5881 ccaacttaat caaagacaaa caaaaataaa actcattctc ctttgagagt ttgacgccac
     5941 aaaatgtagt agtcaagcag ttaaaagcca cacaaaaaca aggtaaataa caggtaaact
     6001 atttttatac ataaaaataa aaacattaaa tatcaataaa ttaaaagcaa tcaaattgta
     6061 taaacttttc ataaaaacac atgtagcaat tttttgttat ttctcattta ctactacatt
     6121 actgcgtgat tgaatgattc tcaattacat atccgatggc gaaataacaa acggatagtg
     6181 cttagtgagc aataagaaac aattttcact cgcgacaatc cgcgagtttt tgggataaca
     6241 gactaaatgg ctatgttcac accttcattc tcaggactca aaggtcgggc gctcttttca
     6301 ctgctttttg cggcaccgat gattcatgca acagactctg taacgaccaa agatggcgaa
     6361 acaatcactg ttacagcaga tgcaaatacc gcaactgagg caaccgatgg ttatcaacct
     6421 ctgagcacct ccacggcgac attaaccgat atgccgatgc tggatatccc gcaggtggtc
     6481 aatacggtta gcgatcaggt tctggaaaac cagaatgcga caacgctgga tgaggcgctt
     6541 tataacgtca gtaacgtggt acagaccaat acattaggcg ggactcagga tgcttttgta
     6601 cgccgtgggt ttggcgcaaa ccgggatggc tccatcatga ccaacggtct gcgaaccgta
     6661 cttcctcgta gtttcaacgc cgcaacagag cgtgtggaag tgctaaaagg cccggcctcc
     6721 acgctgtatg gcattctcga tcctggcgga ctgattaacg tcgtgaccaa gcgcccggaa
     6781 aaaacattcc atggttcggt ttcagccacc tcctccagtt ttggtggcgg cactgggcaa
     6841 cttgatatca caggtcccat tgaaggcact cagctggcgt atcgccttac cggggaagtg
     6901 caggatgaag attactggcg aaacttcggt aaagagcgca gtacatttat tgccccgtca
     6961 ctcacctggt ttggtgataa tgcaacagta accatgctct attcccatcg ggactataaa
     7021 actccgttcg atcgtggaac gattttcgac cttacgacga aacagcccgt aaacgttgat
     7081 cgaaaaatac gttttgacga accgtttaat attacagatg gtcagtccga tctggcgcaa
     7141 ctcaacgcag aatatcatct caatagccag tggacagcgc gctttgatta cagctacagc
     7201 caggataaat acagcgataa tcaggcgcgt gttaccgcgt atgatgcaac gacaggaaca
     7261 ctgacacggc gtgttgatgc aactcaggga tctacccagc gtatgcatgc tactcgtgcg
     7321 gatctgcaag ggaatgttga tattgccgga ttctataatg agattctggg tggggtgtca
     7381 tatgaatatt atgatcttct gcgtacagat atgattcgct gtaaaaaagc taaagatttc
     7441 aatatctaca accctgttta tggtaatacc agcaaatgta caacggtttc ggcgtcggac
     7501 agtgatcaga cgatcaaaca ggagagctac tcagcgtatg cacaggacgc actctatctg
     7561 accgataact ggattgccgt cgccgggatc cgctatcagt attacacgca gtatgcgggt
     7621 aaaggccgtc cttttaatgt caatactgac agccgcgatg aacaatggac tcccaaactg
     7681 gggttggtct acaaactgac gccatcggta tccttatttg ccaattattc gcaaacattt
     7741 atgccgcagt cgtcaattgc cagctacatt ggcgatcttc caccggaatc atctaatgct
     7801 tacgaagtcg gggcaaaatt cgagctattc gatggtatca ccgcagatat tgcgctgttt
     7861 gatatccata aacgtaatgt gttgtatacc gaaagtgttg gtgatgaaac cattgccaaa
     7921 acggcaggcc gtgttcgttc aagaggggta gaagtcgacc ttgcgggagc attaactgaa
     7981 aacattaata tcattgccag ctacggctat accgatgcta aggttctgga agatcctgat
     8041 tatgcaggta aaccattgcc gaatgttcct cgtcataccg gttcgctatt cctgacctat
     8101 gacattcata acatgcctgg caataatacg ctgacatttg gcggtggtgg acatggcgta
     8161 agccgtcgtt cggcaaccaa tggggctgac tattatctgc caggctattt tgttgccgat
     8221 gccttcgccg catacaaaat gaaattgcag tatccggtca cactgcaatt aaacgtcaaa
     8281 aacctgtttg ataaaacgta ttacacctct tccatcgcca caaataatct gggcaaccag
     8341 attggcgatc cgcgtgaagt gcaattcacg gtgaaaatgg aattttgatc aaaaaagccc
     8401 ggtatacact tgtataccgg gctatgtttt attcttctgg cgggaggatt atcagacctc
     8461 aatctctgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt
     8521 cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa
     8581 ctgcaccaga cggcgagtgt tcggatcaag cgtggtttcg cgcaattgca tcgggttcat
     8641 ttccccgaga cctttaaaac gctgaacgtt tggcttgcct ttcttgcgtt ttaattgctc
     8701 aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc cgaggtcaat
     8761 acggtagagc ggtggcagtg caacgtaaac gtgaccgtgt ttcaccaatg cgcggaagtg
     8821 ttttacaaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcggagt cagcatccgc
     8881 caggatacag atcttgccgt aacgaagctg gctcaaatcg tcgctgtcag gatcgatacc
     8941 gatagctacc gaaatatcgt gcacttcctg cgaagccagc acttcgtcgg aagagacttc
     9001 ccaggtgtta aggatcttac ctttcagtgg catgatcgcc tgatattcgc gatcgcgcgc
     9061 ctgcttggca gatccgcctg cggagtcacc ttccacaagg aatagttcgg tacggttaag
     9121 atcctgcgcg gtacagtcag ccaatttacc aggcagcgcc gggccgctgg ttagcttttt
     9181 acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg ccatctctgc
     9241 caacagctcc gccgcctgaa cgttctggtt cagccacagg atgaaggcat ctttcaccac
     9301 gcccgaaacg aatgccgcac actgacgcga agagagacgt tctttggtct gcccggcaaa
     9361 ctgcggatcc tgcattttta ctgacagcac ataggcgcag cgatcccaga tatcttccgc
     9421 cgacagcttt acaccacgcg gcagaatgtt gcggtattca cagaactcac gcatcgcgtc
     9481 cagcaggccc tgacgcaggc cattaacatg ggtaccgccc tgcatcgttg ggatcaggtt
     9541 gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg cccagtccac
     9601 cgcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg tcggtaaacc
     9661 gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc gctgttcggt
     9721 gttgttgatc tcatctttaa aagtgatctc aacgcccggg cacagtaccg ctttggcttt
     9781 cagcacatgc gtcaggcgtg aaacagaaaa acgcgggctg tcaaagaagg tttcatccgg
     9841 ccagaagtgc acgctggtac cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc
     9901 ttgcaccttt tcgccatttt caaaggcgat gttatagatc tgaccatcgc ggcgtacgtt
     9961 aacttctacg cgcttcgaca gggcgttaac taccgaaatc cccacgccat gcaggccgcc
    10021 agagaactgg taatttttgt tagagaattt accgcccgcg tgcagacggc aaagaatcag
    10081 ttcaaccgcc ggtactccct cttccgggtg aatatctacc ggcatcccgc gcccatcgtc
    10141 aatcacttct aacgactggt cggcatgtaa gattacatcc acgcgttttg cgtggcccgc
    10201 cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag ggcgagtggt
    10261 atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta cctcaatggc
    10321 atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat cgtcagagat
    10381 ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt
    10441 ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc gcctggcggt
    10501 aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc gcttccagcg
    10561 ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca tattgctgcc
    10621 cggtgtaggg gttctcgttc tgaccgagat agtccgtcag cagttcaaac gggcgcaccg
    10681 ccgggttgac caccactgcg ggcagcataa aacattgcga caaccaggtg gcgtaatatc
    10741 cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg acaatggatt
    10801 ccagcagttc tgccgcgtca gaaggatacg ggggcaactg cggaatgatc atctcaacgt
    10861 cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag cgcggcgagc
    10921 tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag cggtatcagg
    10981 ttggaagcgt gcgtccgcca ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa
    11041 ctcgagagta cgccagccgg gcgcgatggt gtccagcgta aagttagaac agtgcggctt
    11101 aaactgcaca caggtcgacg gcgttgccag caagcggcga ccgttccagt cgagatccag
    11161 ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg ccagcacggt
    11221 atccagttcg cccgcgttac gcaggctgtg ttgatcaagc caactacaac ccgcaggtag
    11281 cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca gtttacgttc
    11341 cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca cctggctatc
    11401 cagcaacagg atttgccatt gctcaccaat aaacacgcgc ttcgccgggg agatacccgc
    11461 atcctgtaac gcactgtaca tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca
    11521 gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag aggattgatc
    11581 ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctggtgtg gccgaatcgc
    11641 ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagggctt cgtgcttttg
    11701 tgcaaacagg tgagtgtcgg taatttgtaa aatcctgact ctgacctcac cagccagagg
    11761 aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctaat aaaccggaat
    11821 cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact gattaatttg
    11881 atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg ctttgaagcg
    11941 aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca gacgcaccgt
    12001 cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg tcaccagggt
    12061 agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca cctgatagct
    12121 tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat
    12181 ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt atgcccactc
    12241 attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt
    12301 gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca catgaacgcg
    12361 aatatcttcg ttttcatcag ccagaccgtg aataccgctt gcggtcgtgg cgtccacttc
    12421 gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga aacttaacac
    12481 cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac gggcaacatc
    12541 ttccacactt tcaccctctt caatcatccc ggcaaccatc tccagtagcc agggggtttc
    12601 gctggtgtcg tacgcggcaa tccgaatctg ctcaatcagc acaacttcat cacgtactgg
    12661 gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg
    12721 actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg aaaaaaagcc
    12781 gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg taacgggcag
    12841 gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa tgtgaatttc
    12901 agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat taacgcccta
    12961 tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca ataattttac
    13021 agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa attgctcccc
    13081 attcttatcg gcctgagcct ttctggtttc agttcgttga gccaggccga gaacctgatg
    13141 caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt
    13201 gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca gctaggttta
    13261 ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa ctctaacgcg
    13321 accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta
    13381 acgctgcagg aaaaagcagc tgggattcag gacgtcacgt atcagaccga tcaacaaacc
    13441 ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgcgattga cgttctttcc
    13501 tatacacagg cgcaaaaaga agcgatctac cgtcaattag atcaaaccac ccaacgtttt
    13561 aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcacagta cgataccgtg
    13621 ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca gctgcgccag
    13681 atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac
    13741 aaaccacagc cggttaacac gctgctgaaa gaagccgaaa aacgcaacct gtcgttgtta
    13801 caggcgcgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca ggatggtcac
    13861 ttaccgactc tggatttaac ggcttctacc gggatttctg acacctctta tagcggttcg
    13921 aaaacccgtg gtgccgctgg tacccagtat gacgatagca atatgggcca gaacaaagtt
    13981 ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca ggtgaaacag
    14041 gcccagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg tagcgtcgtg
    14101 caaaccgtac gttcctcctt caacaacatt aatgcttcta tcagtagcat taacgcctac
    14161 aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg ttactcggtc
    14221 ggtacgcgta ccattgttga tgtgttggat gcgaccacca cgctgtacaa cgctaagcaa
    14281 gagctggcaa atgcgcgtta taactacctg attaaccagt tgaatattaa gtcagctctg
    14341 ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa accggtttcc
    14401 actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc tgatggttat
    14461 gcgcctgata gcccggcacc agtcgttcag caaacatccg cacgcactac caccagtaac
    14521 ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt ctgaacgtaa
    14581 ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt caatttcgac
    14641 cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa aatgaaacgg
    14701 acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca tctgacacca
    14761 gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag tgatgaaaca
    14821 gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa
    14881 tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc
    14941 acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc accagcccag
    15001 gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag tttctggatg
    15061 ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg atttgcacag
    15121 cagccgctgt tctcctcgaa aaatccggcc agcccggctt acggtaaata taccgacgcg
    15181 accggtaaaa actatggcgc agcccagcca ggccgaacca tgaccgtacc gaagacggca
    15241 atggcaccaa aaccggcgac cactactact gttacccgtg gcggttttgg tgaatctgtt
    15301 gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc aatgggtggc
    15361 tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgcgaa aaagcccacg
    15421 aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa gatgcttact
    15481 acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga
    15541 tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc aaattccgca
    15601 ttccaaaaca cacctggagt tttgtgcgcc agtcatggct gacgcaccag ccatcgcttt
    15661 attcgcgtct tgatctggca tgggatggta ctggcgaacc taaacttctg gaaaataacg
    15721 ccgatacgcc aacgtcacta tacgaggcgg cgttctttca gtggatctgg ctggaagatc
    15781 agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtttg caagaaaagc
    15841 tgatcgatcg cttcgttgag ctgcgtgaac agtatggctt ccagttgctg catctcacct
    15901 gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac tgcgcgacgg
    15961 aagctgaaat cgctaccgag ttcctctaca tcgacgatat cgggttaggt gaaaaaggtc
    16021 agttcacgga tttacaggat caggtgattt ccaacctgtt caagctgtat ccgtgggaat
    16081 ttatgttgcg tgagatgttc tccaccaagc tggaggatgc aggcgtacgc tggctggaac
    16141 cggcgtggaa gagcattatc tccaacaaag cgcttctacc gctactgtgg gagatgttcc
    16201 cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg caaatggaaa
    16261 aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg
    16321 gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt gttcagcaat
    16381 tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg ctggtgaacg
    16441 atcaacctgc cggaattggc attcgtgaag accgtgcatt gatcacccag gatatgtctc
    16501 ggttttatcc gcatattttt gttgagtaat acagcttaat attgccccga taagcggggc
    16561 aatctcttat aatccaccat tcataactcc catcaatata taaacaca
//
LOCUS       DAJOTB010000106        16534 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJOTB010000106 DAJOTB010000000
VERSION     DAJOTB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.106"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            96..1568
                     /locus_tag="OHN28_003377"
     CDS             96..1568
                     /locus_tag="OHN28_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2298710.1"
                     /translation="MSGNESIDAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVVGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPQS
                     AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMADRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPKWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSIADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            1565..2581
                     /locus_tag="OHN28_003378"
     CDS             1565..2581
                     /locus_tag="OHN28_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCQ2298711.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECTACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALREAFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFDFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            2592..3602
                     /locus_tag="OHN28_003379"
     CDS             2592..3602
                     /locus_tag="OHN28_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCQ2298712.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            3675..4658
                     /locus_tag="OHN28_003380"
     CDS             3675..4658
                     /locus_tag="OHN28_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCQ2298713.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILKSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSVKNLELVESM"
     gene            4700..5182
                     /locus_tag="OHN28_003381"
     CDS             4700..5182
                     /locus_tag="OHN28_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2298714.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNFH
                     "
     gene            5193..6497
                     /locus_tag="OHN28_003382"
     CDS             5193..6497
                     /locus_tag="OHN28_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCQ2298715.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKTLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(6568..7980)
                     /gene="ftsP"
                     /locus_tag="OHN28_003383"
     CDS             complement(6568..7980)
                     /gene="ftsP"
                     /locus_tag="OHN28_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCQ2298716.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(8055..8792)
                     /gene="plsC"
                     /locus_tag="OHN28_003384"
                     /gene_synonym="parF"
     CDS             complement(8055..8792)
                     /gene="plsC"
                     /locus_tag="OHN28_003384"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCQ2298717.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(9027..11285)
                     /gene="parC"
                     /locus_tag="OHN28_003385"
     CDS             complement(9027..11285)
                     /gene="parC"
                     /locus_tag="OHN28_003385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCQ2298718.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDCEE"
     gene            complement(11423..13030)
                     /locus_tag="OHN28_003386"
     CDS             complement(11423..13030)
                     /locus_tag="OHN28_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2298719.1"
                     /translation="MYTQNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRSALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(13139..13621)
                     /locus_tag="OHN28_003387"
     CDS             complement(13139..13621)
                     /locus_tag="OHN28_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCQ2298720.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(13674..14066)
                     /gene="ygiW"
                     /locus_tag="OHN28_003388"
     CDS             complement(13674..14066)
                     /gene="ygiW"
                     /locus_tag="OHN28_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCQ2298721.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            14218..14877
                     /gene="qseB"
                     /locus_tag="OHN28_003389"
     CDS             14218..14877
                     /gene="qseB"
                     /locus_tag="OHN28_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCQ2298722.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14874..16223
                     /gene="qseC"
                     /locus_tag="OHN28_003390"
     CDS             14874..16223
                     /gene="qseC"
                     /locus_tag="OHN28_003390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCQ2298723.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(16269..>16534)
                     /locus_tag="OHN28_003391"
     CDS             complement(16269..>16534)
                     /locus_tag="OHN28_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCQ2298724.1"
                     /translation="DSFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT         4155 a         4133 c         4055 g         4191 t
ORIGIN      
        1 ttacctttct ctgcaagcgt gatgcatctc tctgtttttg gtctagtgaa ttatgtaaat
       61 tggtctaaac ataagccacc aggagtaaga gcataatgtc agggaacgaa agtatcgatg
      121 ccgcccacta tgatcaggta accacctggc ctcgggacgg gttacaggca gatattgtcc
      181 atattggttt tggcgctttt catcgcggac accaggctgt ctacacggat ctcactaatc
      241 aactttcgga cacccgctgg gggatctttg agatcaacct gtttggtgat gctcaactga
      301 tcgaaaactt aaatgcgcaa aatgggctgt tttcggttgt ggaaacatct gcatcgcaat
      361 ctacctcacg ccttgtgcgt tcggtggttg gcggtattca tacccccaga gatggcattg
      421 ccgcagcaat ccataaactg gctgaacctc aggtaaaaat cgtttcatta actatcaccg
      481 agaaaggcta ttgtctcgat ccgcaaacac gttcgcttga tctcaccaat ggattaatcc
      541 aacacgatct acaaaacccg gatgcgcctc agtcggctat tggcttgatc gtctgtgctt
      601 tgcaacaacg taaagcggca ggactcgccg cttttagtgt gctctcctgt gacaacttgc
      661 cagacaatgg gcatctgacg cgcaatgccg ttctcggttt tgcccgacaa ctggaccagc
      721 ccctggctca gtggattgaa gaaaatgtct catttccagg cacgatggcc gatcgcattg
      781 ttccggcaat gactgaatcg caattcgcct tactggaaac taaaaccggt tatgccgatc
      841 cctgcgggat cgtctgcgaa tcatttcgtc agtgggtgat cgaagataat tttgtgcgcg
      901 gacgaccgaa atgggataaa gccggcgcga tgtttgtcag caatgttcag ccttatgaag
      961 agatgaagtt acgcatgtta aatggtagcc attcatttct ggcttataac ggctctttgg
     1021 caggctatga gtttatctgg caatgcatgg aagacgctaa ttttcgttcc attacccgcc
     1081 aactgatgat taatgaacaa gcccgaacac ttaatccaga cttaaatatc aacatccagg
     1141 aatacgccga cctgttaatt gaacgcttta gcaaccgtaa cgttgcacac cgtaccgggc
     1201 aaatagccat ggacggttca caaaagcttc cccagcgtgc actgacgccc tggctgaaat
     1261 tacatcagca aaagcaaaac aatgctgttc tgtcactgct cgttgctggt tggttgcatt
     1321 atgtcattga tgctgttgag aaaagccagt ctatcgctga tccaatgaat gaccaatttc
     1381 aggcgctaat aaaggaacaa caagacgcat ggcaacaggc gctcgcatta ctgcacctta
     1441 gcgccatatt tggtgattta agcaaccatc agccatttat aaatgaaata aaaatcgcct
     1501 ttgcaaatat aaaaaacaaa ggcatcaagg ccaccatcag ccaattatta tcggatgagc
     1561 agaaatgaaa acattaattt gtcagcagcc tggcgttatg gaatatgtgg aaaaggatat
     1621 tcccacacca gcagataatg aagtgctgtt aaaaataaaa gctgtgggta tttgtggtac
     1681 tgatattcac gcttttgccg gcagacagcc tttttttagc tacccacgtg tattaggtca
     1741 tgaaatatgc gccgaagcgg tttcgcgagg cagccagtgc caaacagcac aatcaggcca
     1801 gcgctattcc gtcatcccat gcattccgtg tggcgagtgc acagcctgtc gggaagagaa
     1861 aacgaactgc tgcgaacgtg tttcgctgta tggcgtgcat caggatgggg gttttagtga
     1921 gtaccttgcg gtacgtgaag acaaccttgt gcctctacct gacgaggtca gcgacagtgc
     1981 cggagcattg gttgaatgtt tcgccattgg tgcacatgcc gttcgtcggg cagagatcaa
     2041 ggctgaacaa aacgtactgg tgattggtgc tgggccaatc ggtctggcta ccgcagccat
     2101 cgccagggct aaaggggcgc atgttgttgt tgctgatatt gactgtcaac gtcgccagca
     2161 cgttgtggat catctggcaa ttaatgtctt cgacccaaca caggaaggtt ttattgccgc
     2221 gcttcgtgag gcctttggag gcgaactggc ttgcgtagtc ctggatgcga cgggaaataa
     2281 agcttcaatg agtcatgatg tcaatcttat tcgtcatggc ggcaaaattg ttttcatcgg
     2341 tttgtacatt ggtgaacttg ttattgacga tccgaccttc cataaaaaag agacaacgtt
     2401 actcagcagc cgcaatgcca cacgggaaga ttttgcgttg gtgattgaac tgatgcgcag
     2461 caataaaatt cacgaaaatt taatgaaaaa ccaggcgttc gatttcttta gtgttggcga
     2521 agattaccag cgtaacgttg tagaaaataa aaatatggtc aaaggtgtga tcacttttta
     2581 agggaacaaa tatgacaact gtttatgtca gtgaagaaaa tttaaaatca ctggtgcacc
     2641 acaaattaca taccgccggg ctggataccg acaccacaca acaggtgaca gatgttttag
     2701 tacatgccga tattaccggt gtgcattcac atggtgttat gcgtgtcgaa cattattgca
     2761 cccgccttgc tgccggaggg ttaaataaag ccccgcagtt tagcattgag caaatttcac
     2821 cgtcagtggc cattctcgac tctgacgacg gaatggggca ttcagcatta attagcgcca
     2881 ctgagcacgc tattaaactg gcccagcaag agggtctcgg ttttgttagc gttaaaaaca
     2941 cgtctcactg tggtgcgtta tcttattttg cagagatgat caccaacaaa gggttggttg
     3001 ctatcgtaat gacgcaaacc gatacctgcg tggctcccca tggcggcgcc gagcgctttt
     3061 tgggaactaa ccccatcgcc tttggtttcc cggtggaaaa cagccatccg atgattgttg
     3121 atatggcgac cagtgccacg gctttcggca aaatacttca tgcaaaggaa accggaaaac
     3181 atattggtga agggctggcg atagataaaa acggttatgg caccactgat ccgtataaga
     3241 ttgaaaacct gctacctttc ggccaacaca aaggttcagg cattgcactg gctattgatg
     3301 cactgactgg cgtgctgatg aatgcgaatt ttggcaacca tattgttcgc atgtatggtg
     3361 attatgacaa gatgcgtaag ctggcaagtt tggttattgc catcgatccg aaaaagctcg
     3421 gcaatcctgt ttttgcaaaa actatggcga aaatggtcac ggagctgcat gccgttaaac
     3481 cggctcctgg tgtcgaaaaa gtgttagcgc cgaacgatcc gcaaatgcac tacaaagaaa
     3541 aatgccaaca ggaaggtatt ccggttccgg caggaatatt ccattatctg gcagagaatt
     3601 aatcactatt ttttattata acgatagaaa agcgctgtat ttatcgtttc tgttatatta
     3661 accaggagca ggatatgaaa gcacttcgtc ctctgacggc atcgcttatg ctgttaacca
     3721 gttgtttatt aatatctaat accacactgg caaaaacaac attaaaactg agtcataatc
     3781 aggataaaag ccatgctgtc cataaagcat taagctattt agcggataaa accaaagagt
     3841 attctaatgg tgaattagtt attcgcattt acccaaatgc gacattaggt aatgagcgtg
     3901 aatcactgga attaatgaac tccggtgcat tacagatggt taaggttaat gccgcatcac
     3961 tagaatcatt tgctccggat tacagccttt tcagtctgcc gttcttattc cgcgaccgtg
     4021 atcactatta tcgtgttctg caaagtgatt taggtaaaaa aatacttaag tcatcagaaa
     4081 gcaaaggttt tgttggtatt acgtactatg acggcggtgc gagaagtttt tattccaata
     4141 aaccgattac aaaacctgaa gatttagcgg gaatgaaaat cagggttcaa caaagcccca
     4201 gcgccattgc aatgatgaaa gcactcggtg gtgtcgctac cccgatggcg caaggcgaac
     4261 tctacaccgc acttcagcaa ggagtggttg atggcggaga gaacaatacc gttgtctatg
     4321 ccgatatgcg tcacgccgaa gtcgcaaaag tctattcacg tgatgaacac accatggtac
     4381 ctgatgtgtt aattatcagc accgacgtgt tgaataaact tggtgataaa gagcgcacgg
     4441 cattattaaa agcagccgat gagtcaatga tgcagatgaa ggacgtcatt tggcctgcgg
     4501 ctgaaaaaga agcctacgac aaaatgaaag ggatgaacgc aacagttgtt gatgttgata
     4561 aatccgcttt caaagaacgc gtcaaaccgt tatacgatga attcaaggcg aaagatgcac
     4621 aatcagtcaa aaaccttgag ctagtagaaa gtatgtaacc ctaatgggca ggtaattcct
     4681 gccttttcat ggaagactta tgcgaacact cactcatatt cttaataaga tccttgccgg
     4741 atgttgctgc attattctcg ccattatggt gttctgcgta acctggcaag ttattgcacg
     4801 ttttattttt aacgctccca gcactgtgct tgatgagttc acccaaattt tatttatgtg
     4861 gatgatttta ctcggcggtg tatatactgc ggggttaaaa aaacatctgg ccattgattt
     4921 gttggctcaa aaacttcctg ctgcatctgt tctgacgctt gacagtttta tccaaattat
     4981 cattacggtc tttgcggtta ttttcatgat ctatggcggg aatattgttg ttgaaaaagc
     5041 acaacatgtt ggacaaatct ctccagtatt aaaatggcca atggataaag tatattgggt
     5101 tatgcctgtc agtgggctta ttctggtctg gtattcagta atgaatatta ttgacaacta
     5161 tcgtaaacgg aattttcatt aacgagggaa atatggactt tgaatatatc taccccgtct
     5221 taattttatt tggcagtttt gccgtcatgc tggcaatagg tgtgccaatt acttttgcga
     5281 ttggtctttc ttcgctgtta tctattatta ctgccttacc acccgatgcc gccatttctg
     5341 ttatttcaca aaaaatgact gtggggctgg atggctttac gctattagcc attcccttct
     5401 tcgtgttagc cggaaacatt atgaataccg gtggtatagc cagacgactg gttaacctgg
     5461 cgcaagcatt agttgggcgt cttcctggct cactggctca ttgtaatatc ctcgcgaata
     5521 cactgtttgg tgcgatttca ggttcagccg ttgcgtcagc cgcagcggta ggtgggatta
     5581 tgtcaccact gcaagaaaaa gagggatatg atccggcgtt ttcagcagcg gttaatattg
     5641 cctctgcccc cattggcctg atgatcccac cgagtaatgt gttgattgtt tattccctcg
     5701 ccagtggcgg gacttctgtc gctgcgttgt tcctagctgg atatctgccg ggcatcctca
     5761 ccgctgttgc cttaatgttt gtagctgcac tttatgcacg acgtcaccat tacccagtgg
     5821 ccgaacgcat caattaccgt cagtttttac aggtattccg cgaatcaatt cctagcctga
     5881 tgcttatttt tatcattatt ggcggtatta tcgcaggggt atttacgccc acagaagcat
     5941 cagcaatagc ggtaatttat agtttagccc tggcgatgat ttaccgggaa atcactctta
     6001 agacgctcaa tgatattctg ttagattcgg tagtaaccag ttcaattgtt ctgttactgg
     6061 taggctgctc gatggggatg tcatgggcca tgacgaatgc tgatgttcct gagttgatca
     6121 acgaactgat tacccgcgtt tcggataaca aatgggtcat tctgtttatt atcaatatca
     6181 ttctgttgat cgtcggtacc tttatggata tcacgccggc gatcctgata tttacaccta
     6241 ttttcctgcc gatcgcccag catctgggga ttgatccgat tcacttcggc atcattatgg
     6301 ttttcaactt aaccattggc ctttgcacac cacctgtcgg caccattctg tttgtggggt
     6361 gcagcattgg taaggtcagt atcgacaggg caataaaacc attactgccg atgtttctgg
     6421 cattgtttgt agtaatggca attatttgtt atttcccgca gcttagtctg atgttgccag
     6481 gattattttc gacctgatac acaatgaaga ctgcatactg agtaattttc aatgccccac
     6541 cactctgtgg ggcattgaat tggtccatta cggtaccgga ttgaccaaca gttgcccaat
     6601 cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca
     6661 ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt
     6721 ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct ggaacatcac
     6781 gccttcaata tggaacgctt gcggctcgtc cgcgcgcacc gtccagcgtt cccacgttcc
     6841 ttgctgcgca gtaacatcaa tacggttgac gtcccacaac tgcccattaa tccccgggtc
     6901 atcacccaga ctgatatcac gacttcgaat cggcgaaccg gccatgattt cagttggcag
     6961 caagcgcatc ggaagactgt cagtgaccag cggcagaagg ccggttggac gcagcgttag
     7021 caccagcgta gaaaccagaa tgctggatgg ctcaaagaag ccacgaatac gatcaacaat
     7081 gctcgccgct tcgccacagg tgatcgacac ttcatcaccg ttgctcatat ccaccagaat
     7141 ctcacgacgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag cgggcaggaa
     7201 tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg
     7261 acgagagttc gacgcattca acagacgcaa gcgcacccag ccacgcgaga cttcaacgta
     7321 cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc
     7381 gttgtattct ggcgtaccaa agttatccag ccgtttatcc tgaataatga caggaaaatc
     7441 atccacacca taatggttgg ggataggcag cgatttactg acttcatctt ccaccagcca
     7501 cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata
     7561 ccacagagta gctgcgttct ggcgaatggg cagtacgggt gcccagtcag cgtttggcga
     7621 catcatccgt gccggaccgc ccatcagcgg accaggtacc tgtagcccgg ccaccgtcat
     7681 tgagacattt tctgtcaggc gattgctgta aataagctta acatcgtcgc ccttccagac
     7741 gcggatagtc ggccccaggt aacgaccatt gattccccaa accgatgcgc gtgtccctgg
     7801 cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc
     7861 aagcagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac
     7921 agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat
     7981 atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa
     8041 tacagattgc cgacttaaac ttttccggct gcttcgcgtt ctgcgacttc tttatcgagc
     8101 tcggcgattt tttgttccat tatcgaacga caatgggcag ccaactcgcg aacctgatct
     8161 ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgt
     8221 agccgattaa gattaatctt attcgaagtt gtagagacgc acacgggtat aatcgggacg
     8281 cccgccgcaa ttgccgcgtg aaatgccccg gtcttgaacg gcagcaggcc gcgaccacgg
     8341 ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa gtgattcact
     8401 acttccgcaa tagtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg
     8461 gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc
     8521 actgtcggcg gttgcacgat gttcgatgcc gtcaccatgt catagttgtt ctggtggtta
     8581 gcaatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc
     8641 aggccaaaca gcggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg
     8701 ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc
     8761 acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg
     8821 cattgtacgt cacctgaaaa tcaggttagg gcattgttaa aagccgctct ctggaaaagc
     8881 gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt atcgtttccg
     8941 gggattgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg
     9001 gaggaaacag cgccctcccc ggcatattac tcttcgcaat caccgctgct ggcacggcga
     9061 ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt
     9121 ccacgttcgc cagcgacttt ctgtagctct tccggacgca gtttaatttt gcgtttccca
     9181 acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca gttgcgccag gccatcctcg
     9241 ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc
     9301 tgcggcagat cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc
     9361 atatcggaag catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg
     9421 atcaaagcct tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg
     9481 taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca
     9541 acggtcgctc cagggggcaa cgttaatttg ccggtgagcg gctcgccctg accacgcgcc
     9601 gacggcagcg taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc
     9661 ggttggttgc tcttaccttt caccgccgct ttgaagctat cgcccgcttt ataattcagg
     9721 cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca
     9781 atggtgacag gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt
     9841 tcctgcaacg gcgaacgacg ttcgtcaccg taggcttgcg cgtctgcctg cagttctttc
     9901 ttcagcaggt tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct
     9961 ttttccagct cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc
    10021 agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc
    10081 gccggtttcg gttcatcttc attacgaatg atctcaatca cttcgtcgat attgagaaac
    10141 gccaccagca aaccttcgag gatatgcaag cgcttgagga ctttctccag acgatagttc
    10201 agtcggcggc gcacggtatc gcggcggaac accagccatt cggagaggat ttccagcagg
    10261 tttttcaccg ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt
    10321 tccagatcgg tggtagcaaa gaggtggttc atcacctgat ccatatccac gcggttggag
    10381 cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca
    10441 accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac gcgcgcacct
    10501 gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt ccaaaccgcg
    10561 cgcatacgca ctgaaccacg tccgttctcg tagattttac gaatctcggc gcgcgaagtg
    10621 ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc
    10681 gtggttttcg gctggtcgat taatgcgatt gccgcctgag ccacttcacg caggttatgc
    10741 ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt
    10801 ggcagacggg caggtagcat tttcggctcc tgcaaagtgc cgtcgaagtt tgggacccag
    10861 tcagccgtcc cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat
    10921 tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcacccca gttcccctga
    10981 ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa
    11041 caggcactat cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat
    11101 tttttaaatt tggcgctggc attcaggccc agctcagaca tcgcatacac aatgcgccgc
    11161 tgaacaggtt tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg
    11221 gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg
    11281 ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat attgccggag
    11341 atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg
    11401 tgctccggcg gcgataaagg catcaatgtg ccttgatata caactctttg ctgtagacat
    11461 aatcctgggg attatgcagc ggaaaaccgc caacgtaggg tttaagcagt ttgattaacg
    11521 gctgatagta gatgggaatc agcggtgcct gctggttgat gatcacttct gcctgctgat
    11581 acaacgcact acgctttgtc gcatcagtga tctgcgtggc ctggtttagt aaggcgtcat
    11641 actgcgcatt tttccagtga ccgacgtttt cttcactatc gcttttgagc gtgttcagga
    11701 agctggaggc atcattgtac gtcgcatccc acgactgccg agacagcata aaatcaccgg
    11761 ctcgtcgggc atcaagatag gttttccact ccattgtgcg cagcgtcacc tgtgcaccca
    11821 gccatttttt ccattcggaa gacaacgcta tcgcggtctt ttcatgcaga tcgtacttgt
    11881 tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta tcccgcctgt ttcagcaaga
    11941 cttttgccat cgcgacgcgc tcactcattg gcttttgcag ttcatcgaac gtcgtcgcgc
    12001 taaagccttt tacctctggc ggcgtcagcg tggttgcggg cgttctcaac cccagtacct
    12061 tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc ccgacgcacc cgcacatcgt
    12121 taaatggcgg tttctcaagg ttaaagttgt aatattcgct gttcagacgc gggataattc
    12181 gtagctcgcc aggcagtgat ttttcaatgg cgggaatttg ctgcgccgga acccaggtga
    12241 gatcgacctc tcccgcgcga tagcggttat agccggtgac cgaattatcc agcgccagat
    12301 actcaacctg ttgcaatact gtatgttgcg catcgcggta ctttggattt ttgcgtgcag
    12361 taatcttttc gttaactacc catttatcaa gcacaaaggc accgttgtaa accatgttct
    12421 ctggcttact ccagctatcg ccatgtttag cgatgacatg atgaggaacc gggaacagcg
    12481 tcggccaggc gagcatcgtc gtgaaccaag gaactggctg ctcaagcgta acttcaagag
    12541 tacgatcatc catcgctttg acacccagcg atgtaacatc tgctttaccc gcaacaatag
    12601 ccgcggcatt gttaatgtgc gcctgtgcca gatagccagc aaaagggctt gccgttttcg
    12661 ggtcaaccgc acgttgccag ccgaggacaa aatcctctgc cgtcagaggc tgaccgtctg
    12721 accactgcaa accgctacgt agatggaaaa tatagcgctt gccgccgtca agtatctccc
    12781 agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc catccatacc agaccttcaa
    12841 acagatccag cacaatctgc gcggcagtat tctcctctac cttttgcggg tcgagcgtgc
    12901 ctgggtcgct atgattgttg taacgaaaga cttgttgcgg ggcgagcggt gtgttggcgg
    12961 gaacgtcagc agcgtagaga ggagccgcac ttaccaggct gaccagccat aataaatttt
    13021 gcgtatacat ggtattcctt accggagaaa ttataagccc gacaacaata cgcctgtggc
    13081 attgtttgct caaacttatc gcgagtgatt tggctcacac tccggtaaag attccccatc
    13141 acgccaacgg cacataaata tccgtttcca gtcgccgctc tgtcatccct tcggcaagat
    13201 tggtgtagtg aaacaggatc ggggctttgc gcattttttc gccgcttgca ggtagccagt
    13261 ggcgaatgat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaaca tggcgggcaa
    13321 cagcataacg cccaccggta agttcgccat tactcacgcc ataacgatta tcggggatcg
    13381 gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg gtggctgggt
    13441 cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc gtctctttac
    13501 gccacatgat aaatttcgcc acgctgtagt tgagcaactc agggctacag cggtgcggca
    13561 acatcgccac agggattgat gggaaatcga taatatttac atccagtgtc aggtttgtca
    13621 ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa caattacgga
    13681 tttactttgc ggatctgttt gacgtcaatt tcaacagagt tccagtcttt atcgacttcc
    13741 ccctgaatct caaccgtatc tttcggcgtc accgtcacgc cgttccagcg tttgtgatcg
    13801 atatccacat tgatagtacc gctggcatct ttgaacacgt agagatcgtc agagatgcgt
    13861 tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt tgcgctttct
    13921 acggtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg cgttgccgat
    13981 gggccagaaa aacctccctg ctctgctgcc ataaccggtg cactgcacag ggccattact
    14041 gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg tttccgagca
    14101 tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta atgctgtaca
    14161 gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga tgaaaaaatg
    14221 cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac gggccttagt
    14281 aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga ggctctatat
    14341 agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga tggtcgcgat
    14401 attttgcgcg aatggcgaga aaaaggtcag cgtgagcctg tattgatcct gactgcgcgc
    14461 gatgcgctgg cggaacgtgt agaagggctg cgtctgggag ctgacgatta tctgtgtaaa
    14521 ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg aaccaacggc
    14581 caggccagca acgagctgcg ccacggcaac gtcatgctcg accccggcaa acgtatcgcc
    14641 acgctggctg gcgaaccctt aacgctgaaa ccaaaagaat ttgccctgct ggaattactg
    14701 atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact gtatacctgg
    14761 gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc
    14821 ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga gaaatgaaat
    14881 ttacccaacg tcttagtctg cgagtcaggc tgacgctaat ctttttaatt ctggcctcgg
    14941 tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac gtcgatgagt
    15001 tgttcgacac ccaactgatg ctgtttgcca aacggttaag tacgctcgat ctcaacgaaa
    15061 tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt catgttgatg
    15121 acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt aacgatggcg
    15181 ataacggaga agatattcct tatagctatc agcgggaagg ttttgctgac gggcaactgg
    15241 tcggtgaaga cgatccctgg cgttttgtct ggatgacctc acctgatggc aaatatcgca
    15301 tcgttgtggg tcaggaatgg gaataccgtg aagacatggc gctggcgatt gttgccggac
    15361 aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg gtactactgg
    15421 gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt gaccctgact
    15481 cggaaaaacc actaaacgcg acgggcgtac ccagcgaagt gcgcccactg gttgagtcgc
    15541 taaatcaact gttcgcccgc acacatgcga tgatggttcg tgaacgacgc tttacctccg
    15601 acgcagctca cgaacttcgt agcccgttaa cggcgctgaa agtgcaaacc gaagttgctc
    15661 agctctctga cgatgatccg caggcgcgga aaaaagcgct gctccaatta cattccggga
    15721 tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg gactcactgg
    15781 ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg tcggtgatgg
    15841 atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc aaagccaacg
    15901 gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat ttgctggata
    15961 acgccgtgcg ttacagtcca caaggtagcg tggtagacgt cacgctgaat gccgataatt
    16021 tcatcgtgag ggataacggc cctggtgtga caccagaggc actggcgcga attggcgaac
    16081 gcttctatcg cccacccgga caaaccgcta ccggcagtgg acttgggcta tcgattgtcc
    16141 agcgaatcgc caagctgcat gacatgaatg ttgaatttgg gaatgcggaa caaggtggat
    16201 ttgaggcgaa ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat
    16261 agactttact aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaacccaga
    16321 caaataacga gcgagaatac atatagccaa aactcgaaca tttcccgcat aaagagtttt
    16381 cttaagataa gaataataag tggtgtcaga agaaaaaatg ctgcaatcat tgcttgcaat
    16441 atacgatctt cgcctgaaac agaaggtata ctgcaaatat tgtctcccga ctcaagcatc
    16501 cagtcatact tattgaaagc aaaaaaagaa tcta
//
LOCUS       DAJOTB010000107        16460 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJOTB010000107 DAJOTB010000000
VERSION     DAJOTB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.107"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            216..368
                     /gene="mokC"
                     /locus_tag="OHN28_003392"
     CDS             216..368
                     /gene="mokC"
                     /locus_tag="OHN28_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCQ2298725.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(472..1602)
                     /gene="dnaJ"
                     /locus_tag="OHN28_003393"
     CDS             complement(472..1602)
                     /gene="dnaJ"
                     /locus_tag="OHN28_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCQ2298726.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1691..3607)
                     /gene="dnaK"
                     /locus_tag="OHN28_003394"
     CDS             complement(1691..3607)
                     /gene="dnaK"
                     /locus_tag="OHN28_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCQ2298727.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3978..4382
                     /locus_tag="OHN28_003395"
     CDS             3978..4382
                     /locus_tag="OHN28_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCQ2298728.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4408..5121
                     /gene="msyB"
                     /locus_tag="OHN28_003396"
     CDS             4408..5121
                     /gene="msyB"
                     /locus_tag="OHN28_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCQ2298729.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5270..5836
                     /gene="satP"
                     /locus_tag="OHN28_003397"
     CDS             5270..5836
                     /gene="satP"
                     /locus_tag="OHN28_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCQ2298730.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5871..6458)
                     /gene="mog"
                     /locus_tag="OHN28_003398"
     CDS             complement(5871..6458)
                     /gene="mog"
                     /locus_tag="OHN28_003398"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCQ2298731.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6573..7526)
                     /gene="tal"
                     /locus_tag="OHN28_003399"
     CDS             complement(6573..7526)
                     /gene="tal"
                     /locus_tag="OHN28_003399"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2298732.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7805..9235
                     /locus_tag="OHN28_003400"
     CDS             7805..9235
                     /locus_tag="OHN28_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCQ2298733.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMAHGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9305..10081
                     /gene="yaaA"
                     /locus_tag="OHN28_003401"
     CDS             9305..10081
                     /gene="yaaA"
                     /locus_tag="OHN28_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCQ2298734.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10121..10417)
                     /locus_tag="OHN28_003402"
     CDS             complement(10121..10417)
                     /locus_tag="OHN28_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2298735.1"
                     /translation="MKKMQSIVLALSLVLFAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10631..11917)
                     /gene="thrC"
                     /locus_tag="OHN28_003403"
     CDS             complement(10631..11917)
                     /gene="thrC"
                     /locus_tag="OHN28_003403"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCQ2298736.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHGGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11918..12850)
                     /gene="thrB"
                     /locus_tag="OHN28_003404"
     CDS             complement(11918..12850)
                     /gene="thrB"
                     /locus_tag="OHN28_003404"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCQ2298737.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(12852..15314)
                     /gene="thrA"
                     /locus_tag="OHN28_003405"
     CDS             complement(12852..15314)
                     /gene="thrA"
                     /locus_tag="OHN28_003405"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCQ2298738.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15341..15460)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15396..15461)
                     /gene="thrL"
                     /locus_tag="OHN28_003406"
     CDS             complement(15396..15461)
                     /gene="thrL"
                     /locus_tag="OHN28_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCQ2298739.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15675..16361)
                     /locus_tag="OHN28_003407"
     CDS             complement(15675..16361)
                     /locus_tag="OHN28_003407"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2298740.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3848 a         4383 c         4060 g         4169 t
ORIGIN      
        1 cgaggcgtct tcagaaaaag aattgataaa acagaagtga attaaaagcg cagtataata
       61 gcggcgggtg cttgaggctg tctgtctcag gcattggctg aacggcagat agagaaaagc
      121 cccgagtgat attttaccat caacccgagg ccccctatat gcagaacaca tgtagagtgc
      181 ctcttactga ccgaaaggtc aaggagaaga gagcaatgaa gcagcataag gcgatgattg
      241 tcgccctgat cgtcatctgt atcaccgccg tagtggcggc gctggtaacg agaaaagacc
      301 tctgtgaggt tcacatccga actggccaga cggaggttgc tgttttcacg gcttacgaat
      361 ccgagtaaga gcaacggcgg ggagtgatcc ccgccacttt acaggtgctc gcatatcttc
      421 aacgcaccct tttttttacc tggcctgccc acgggcaggc ttttggggag gttagcgagt
      481 caggtcgtcg aaaaacttct tcacaccatc aaagaagctc tttgagcgcg ggctgttgtg
      541 ctcgccggtt gggccaccaa agctttcttg cagctcttgc agcagctgtt tctgcttctc
      601 gttcaggcct accggtgttt cgacgacaac gcggcacagc aaatcaccct gtgcgccgcc
      661 gcggacagac ttgacgcctt taccacgcat acggaacagc ttaccggtct gggtttcgcc
      721 aggaactttc agtttaacgc gaccatcaag ggtcggcact tcgatttcgc cgcccagcgc
      781 cgccatagcg aagttgatcg ggacttcgca atacaggttg ttgccttcac gctcgaaaat
      841 cgggtgctgt ttaacctgaa cctgaacgta caggtcgcct gccggtgcgc cgtgttcgcc
      901 cgcttcacct tcgcccgcaa gacggatgcg gtctccagtg tccacccctg ccgggatttt
      961 aacggacagc gttttgctgc gctcaacacg accatgacca tgacatttgt tgcacggatc
     1021 tttgatcagc gtaccgcggc cctgacagtg tggacaggtc tgctgcacgg caaagaaacc
     1081 ctggcgcatc tgcacctggc cagaaccatg acaggtcgga caggtctgcg gctgtgtacc
     1141 tggttttgca ccgctaccgt ggcaaacgtc acactcttcc agagtcggaa tgcggatctc
     1201 tttggtcacg ccacgtacag cttcttcgag ggtgagctcc atgttatagc gtaaatcagc
     1261 accgcgcgcc gcacgttgac gaccacgtcc gccgccaaaa atatcgccga aaacgtcacc
     1321 aaaaatatcg ctgaagtctg cgccgccgcc aaaaccgccg ccgcccatgc cgccttgctc
     1381 aaacgcagca tgaccatact gatcgtatgc cgcacgtttt tgcgagtcgg tcagaacttc
     1441 ataagcttcc ttgatctctt taaatttcgc ctcggcctct ttgtcaccct ggttacggtc
     1501 cgggtggtat ttcatggcca ggcgtttgta ggcctttttg atttcacgct cttccgctgt
     1561 tttggaaacg cctaaaatct cgtaataatc ttgcttagcc atcttttttg atttgcccct
     1621 agatgaatgc acgggcggag aggaaattcc ccttcgcccg tgtcagtata attacccgtt
     1681 tatagggcga ttattttttg tctttgactt cttcaaattc agcgtcgaca acatcgtcat
     1741 ctttcgcgtt gtttgcagaa gcatcagcac cggcagtctg ctgctgggca tgttgctgct
     1801 gggcgatttc catcagtttc tgggaaacct gtgccagttc ctgcattttc gcttcgatag
     1861 cggctttgtc ttcacctttc agagcagttt ccagtgcagt cagcgcagac tcgatagcag
     1921 ttttgtcgtc agccggcagt ttgtcgcctg cttcttcaac ctgcttacgg gtgctgtgca
     1981 gcagatggtc gccctggttg cgagtctgta ccagctcttc aaacttacgg tcagcttcgg
     2041 cgttagcttc tgcgtcgcgt accattttct ggatttcatc ttcgttcaga ccagaagaag
     2101 ccttgatagt gatcttctgc tctttaccgc tgtttttgtc tttcgcggaa acgtgcagga
     2161 taccgtcagc atcgatatcg aaggtaactt cgatctgcgg catgccgcgc ggtgccgggt
     2221 tgataccatc caggttgaac tgacccagag atttgttatc agccgcacgt ttacgttcac
     2281 cctgcagcac atggatggtt accgcagact ggttgtcttc agcggtagag aacacctggc
     2341 tgtgcttggt cgggatagtg gtgtttttcg cgatcagcgt ggtcatcaca ccgcccatgg
     2401 tttcgatacc cagagacagc ggggtaacgt ccagcagcag tacgtctttt acgtcaccag
     2461 tcagaacacc accctgaaca gcagcaccga tggctacagc ttcgtccggg ttaacatctt
     2521 tacgcggctc tttaccaaag aattcagcaa ctttcttctg aaccattggc atacgagtct
     2581 gaccaccaac gaggataacg tcgtcgatat cagatacgga caggccagcg tcctgcagtg
     2641 caactttcag cggctcaatg gaacggttta ccagatcttc aaccaggctt tccagtttcg
     2701 cacgagtcac tttgatgttc atgtgtttcg gaccggtcgc gtctgcagtg atgtacggca
     2761 ggttaacgtc ggtctgctga gcggaagaca gttcgatttt cgctttttct gccgcttctt
     2821 tcaggcgctg cattgccagc ggatcgttgc gcaggtcaat gccctgatct ttcttgaatt
     2881 cttcaaccag atagttgatc agacggctgt cgaagtcttc accacccagg tgggtatcac
     2941 cgttggttgc cagaacttcg aaggtttttt cgccgtcaac ttcgtcgatt tcgataatgg
     3001 aaatatcgaa agtaccacca cccaggtcat aaaccgcgat agtacggttg ccagtacctt
     3061 tgtccagacc gtaagccagc gcagctgcgg tcggttcgtt gatgatacgt tttacttcca
     3121 gaccagcgat acggcctgcg tctttggttg cctgacgctg agcatcgtta aagtatgccg
     3181 gtacggtaat aacagcttca gttaccggtt cacccaggta atcttcagcg gttttcttca
     3241 tttttttcag cacttcagca gagatctgcg gcggtgccat tttctggcct ttaacttcga
     3301 cccatgcgtc gccgttatca gcagcaataa ttttgaacgg catgatggaa acatcacgct
     3361 gtacttcttc gtcctggaag cggcggccaa tcaggcgttt aatcgcaaac agggtgtttt
     3421 gcgggttcgt cactgcctga cgtttagccg gctgaccaac cagagtttca ccatcctggg
     3481 tataggcaat gatagaaggc gtggtgcgat cgccttcggc gttctccagt acacgaggag
     3541 tggtgccatc cataatcgct acacaagagt tggtagtacc caggtcgata ccaattattt
     3601 tacccatcta aacgtctcca ctatatattc ggtcatcatg tggttgtgag tctgtaatag
     3661 gggcgaaacg tctggtttca actgcccaat ttcatttttt tgcagactca ctcactgcgg
     3721 ttgactacta aatggggtcg taacccacgt catcaagggg gagatgcaaa aattttttgt
     3781 gcttttgcgc agaaatttcg cgatatggca ggacaagacg tgaaactgac agcaatttta
     3841 ttcatataaa gaatgaatcg cagcaaggca ttcatcaaaa aattgtaata aaaagaaaac
     3901 attacgtgcc tgaaacttct ctttatcagc ggtaaactag tttcatcccc ttacccctcc
     3961 ccaccaagag aatccttatg aaatccgtta ttacgatttc cgccagcctg gcgattagcc
     4021 tgatgctgtg ctgcacggcg caggcaaacg accataaaat cctcggcgtc attgcaatgc
     4081 cgcgaaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gttaaacgca
     4141 tacaactctc agccgaccat ggcgatttgc agttaagcgg tgcatcgatt tatttcaaag
     4201 ccacccgaag cgccagtcag accctgaata ttccttcaga aataaaagaa gggcaaacca
     4261 ctgactggat caacattaac agcgataacg acaacaaacg ctgcgtctca aaaatcacct
     4321 tttccggtca tacagtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4381 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4441 gattttctcc agcattgtag tgaggaacag ttggcaaatt tcgcccggct gctcacccat
     4501 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4561 atggaaggac atcccgagcg acaccgccgc aactggcagc tgattgccgg agaattacag
     4621 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgcgcc
     4681 attttgctcg atgtttcaaa gcgattgaag ctgaaagccg acaaagagat gtctacgttt
     4741 gaaattgagc aacagttact ggaacaattt ctgcgtaata cctggaagaa aatggacgag
     4801 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     4861 ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg tgtcgcattt gctttccagc
     4921 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     4981 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5041 ggcagtgcct atcgcgtgac aattccagcc gtactgcaaa tcgcctgcct gcgccggatg
     5101 gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa ttttcatttt cccctcaaaa
     5161 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actacagtta
     5221 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5281 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5341 taacctgcac aacgtgggtt attttgctct ggacggtatt attcttgcca tgggcatttt
     5401 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5461 cggtttaacc gcattcacct cttacggttc tttctggctg acgctggttg caattctgct
     5521 gatgccgaaa ctggggctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5581 tctgtggggc atatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5641 gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg ctggcgatcg gtaacattgc
     5701 cggtaacgcc gcgatcatcc actttgccgg ctggattggt cttatctgcg gtgccagcgc
     5761 aatctatctg gcgatgggtg aagtgctgaa cgagcagttt ggtcgcaccg ttctgccgat
     5821 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     5881 acttcgcgtc ttgcactctt cggtctgaat gctgcaacca cttccggtgc cgtttcaacg
     5941 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgcaccaca
     6001 acgttaccct cagcgtcctt cacaccttcc agcgtctctt taatagactt cggttgaccg
     6061 ggtaagttaa ggatcagcgc ctgtttgcga atcaccccca cctgacgcga aaggatcgca
     6121 gttggtacaa aatgcaggct gatctggcgc atctgttcac caaagcctgg catctcgcgg
     6181 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6241 gtggtaagca ccagatggca actcatttca tccaccagct cacacaacgt ttgctcgatg
     6301 atcgcctgct catcgggaat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6361 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6421 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6481 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggtttca gaagaggtag
     6541 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6601 agtttttcct ggtcaacagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6661 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6721 acttcaccag tgtaagacag tttacgttcg atagctcctt cgctctccgc cagctctttc
     6781 agcagtgccg gtgcgatggt cagacggtcg cagcctgcca gttccagaat ttcgccgatg
     6841 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     6901 atttcagata cagaaaccac gcctggatct tctgccggag cgtactcttt cttatcggta
     6961 ttcgctttgt accagtcaag aatacggcca acaaacggcg agatcaggaa cacgcccgct
     7021 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7081 tctttttcca gctgttccgc agcacggata ccctgccagg tagaagccag tttgatcaga
     7141 atacgatcgt tgctaatacc tgcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7201 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7261 accagtttca ggatttccag accaatattt acggccagtt tgtcggtcgc gtccacgatc
     7321 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7381 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7441 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7501 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7561 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7621 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7681 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7741 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     7801 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggttcgg taatgattta
     7861 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     7921 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     7981 ctcatttcag tcattgtgta ccagtcttgc agcgcgcgtg ggtagcggca acctggccgg
     8041 cgttgcgctg gcaattaccg ccggtggacc tggagccgtc ttctggatgt gggttgccgc
     8101 atttatcggc atggcgacct cgtttgccga atgttccctc gcacagcttt ataaagaacg
     8161 tgatgccaat gggcagtttc gtggcggacc ggcatggtat atggcgcacg ggctggggat
     8221 gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8281 cggagttcag gcgaacgccg ttgcgcgagc cctgagtttt tcttttgatt ttcccccgct
     8341 ggtgacaggt attattctcg ctgtctttgc tatgctggcg atcactcgcg gtcttcatgg
     8401 cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg tactgaccag
     8461 cctggtgatt tgcgtaatga atatcgggca acttccccac gtcatttggt ctatttttga
     8521 gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct taagccaggc
     8581 gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg ggtcgacgcc
     8641 aaacgcggca gcggcagctg cgtcctggcc tccgcatccg gcagcgcaag gaattgtcca
     8701 gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca tgctgatatt
     8761 actggcgggt aacggcacaa cctacatgcc gctggaaggt attcagctta tccagaaggc
     8821 gatgcgggtg ctaatgggtt cctggggtgc ggagtttgtt accctcgtgg ttattctgtt
     8881 tgccttcagc tccatcgttg ccaactacat ttatgccgaa aacaatctct tctttttacg
     8941 cctgaacacc cctaaagcga tctggtgtct gcggatctgc accttcgcaa cggtcatcgg
     9001 cggcaccttg ctaagtcttc cgctgatgtg gcaactggca gatatcatta tggcctgcat
     9061 ggctattacc aatttgaccg ccatattact gctctcgcct gtggttcata ccattgccag
     9121 tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt tgcgttatcc
     9181 ggagatcggt cgccagcttt ctccggacgc gtgggatgat gtttcgcagg agtaatcaca
     9241 actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc tgcaaggact
     9301 ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc cgttgaccac
     9361 cacgcgctat acgctgccgg agctgttaga caactcccag cagctgatcc atgaagcgcg
     9421 gaaactgacg cccccgcaga ttagctccct gatgcgcatc agcgataaac tggcgggtat
     9481 caacgccgct cgctttcatg actggcagcc agatttcaca ccggaaaatg cccgccaggc
     9541 aattctggcg tttaaaggtg atgtctacac tggcttgcag gccgaaacct tcagcgaaga
     9601 cgatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg gcgtactgcg
     9661 cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atacgtcttg agaatgcccg
     9721 agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga acgaggcgct
     9781 cgcagcacaa ggcgataatg tagtgataaa tctggcgtca gatgagtatt ttaaatcggt
     9841 gaagccgaag aaattgaatg ccgagattat caagccagtg ttcctcgatg agaagaacgg
     9901 caaatttaag ataatcagct tctacgctaa gaaagcacgc ggtctgatga gtcgtttcat
     9961 tattgaaaat cggctgacca aaccagagca actaactggt tttaatagcg aaggttactt
    10021 ctttgatgaa gcttcctcca gcaatggcga actggtgttt aaacgctacg agcagcgtta
    10081 agtttgtagt caatgccgga tggcaatccg gcattgatat ttagcgatga tgtttgtcag
    10141 gccccggacg atgatcgcca cgatgatcat tgtgcttgtt atggcgcggc gatggcggcg
    10201 gtccatatgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt
    10261 cgcgccagtg accgccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt
    10321 ttactgacgg gactagcgta atttccgcag cctgcgctgc catgggagcg aacagaacca
    10381 gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc
    10441 cgttatgggt cgatatccgg agattacgcg atgagaacgg cagttgttat tctccgcaat
    10501 cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc
    10561 catattttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa
    10621 tgaatagatg ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca
    10681 ggttatgcga aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg
    10741 tttcaccgag aatcgcttcc acgctctctt taaattttgc cggatgcgcg gtgccgagga
    10801 acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgataagct acggcagcgt
    10861 gcggctccga ggtatagccc agttctttta actcacgcat tgtctgttgc gtggtttcat
    10921 catccacggc tgcatacccc agctctttca gttgccagat tttgcggcgg aacaactctt
    10981 ccacacgcgg ccagttgttt ggctggctaa catccatcgc attggataac gtcgcctgag
    11041 tcgctttggg tgaccactga ccgccgtgca ggaaacgtgg cacggtatca ttcacgttgg
    11101 tcgcagcaat aaaacgcttc accggcaggc caagagattt cgccagcagg cccgctgtca
    11161 aatcgccgaa gttaccgctc ggcaccgaga caaccagctg gttgcgcgct tcctgcggca
    11221 actgcgccac ggcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatgg
    11281 agttagcaga gtttagcccc agcgccactt tcagctcttc atcatcaaac gcctgcttca
    11341 ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca
    11401 atgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca
    11461 ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccagta tcaccggatg
    11521 tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa
    11581 agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga
    11641 catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcacgt acgcgctctt
    11701 ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg
    11761 tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt
    11821 gcggaaaaaa cagcccctga tttttgccta acccctgggt tacggcttgc gcaaagctga
    11881 cctgctcatt gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc
    11941 ccgccgtatc cagctggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac
    12001 ccaaccagtc ggcaacgcgc tgagcggtat ccggcttgtc acacagagcg aacaaggtcg
    12061 ggccggagcc ggagataccg ctcgcaaccg cgccgatttc cgcaaccgcc tgccgcgcct
    12121 gccggaagcc aggcaataac cgttcacggt agggttcagc gataacatct ttcatcagct
    12181 tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaatcctgcc aaatgtcgcc
    12241 cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg
    12301 tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac cctggcactt
    12361 gctgactgat gatgtcgttt tcttcaatca tcaactgcat accaccaaga aaacacggtg
    12421 ccacgttgtc gtaatgaatg ctgccggaga tacgcccttc caactcgccc atcaaagcca
    12481 gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc agcgccgcga
    12541 ccactgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt tccagagtca
    12601 tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac tgataaacga
    12661 tattttcccg tggctctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga
    12721 atgtctctgc tgcctcaacc gtgactacat ctccgagcaa tgcaccatca acaggtgtca
    12781 ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa
    12841 ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc agcaaagaca
    12901 ccggcagctg taacgtcatt gcccgcacca tatccgcgca gtaccaacgg cagcggctga
    12961 taatagtggc tatagaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca
    13021 ttaccatcca cttcggcaat cttcacgcgg cagacgccat cttcatcaat attgccaaca
    13081 tagcgcaaaa cttttccttc atcacgagcc ttcgccacac gcgcggcaaa gagatcgtcg
    13141 agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc
    13201 agcacaggtt caatttcaat atccgccaac tccagttcac gtcccgtttc acgagcgaga
    13261 atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta
    13321 taacccattt cccgcgccag tgtggtcgcc tcggagaaac tcatgccttc gtctaacttg
    13381 ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa ttcatcacca
    13441 gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca
    13501 tagaggaatt tacgccgcga tttttccgcc gcataacgca actgatggta gtaatccatc
    13561 gacgaggtgt tggccttttt gttcggcgta acaacgtgga aaccttcgcg caggaagtcg
    13621 gcatattgat ctgccacagc ctggctggaa gtacagtcaa caatgaccgg gttcagcaga
    13681 tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgcgcc
    13741 agttcttcct gccagttttc cagattaagg ccatgtacat tggtgagcag tgccttcgag
    13801 ttagcaacac cgcagacacg taagtcgata tgtttattct tcaaccagct ttgctgacgc
    13861 ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata
    13921 acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg
    13981 ttattgacca cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatattg
    14041 atgttggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgtaa ggtgcgcata
    14101 ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccaacgg ctccagtaag
    14161 ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg cacacagtcg
    14221 ctttgcggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg
    14281 gaaatacggg cgcgtgacat cgctgcaaag acgcgcgccg ccatgccaac catccctttc
    14341 atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc
    14401 ggtaattcgt cttcatcacg gctggcacca atgagcgtac ctggtgcttg aggatttccg
    14461 gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga
    14521 agaactttag cgccgaagta agaaagctcc atcgcttcct gataggacat cgacttcaac
    14581 aacctcgcat cgggcacctg acgcggatcg caggtataaa caccgtcaac atccgtccag
    14641 atctcgcaac aatcggcgcg taaacaggcc gccagcaccg cagcggagta gtcggaaccg
    14701 ttgcgtccca gaaccaccag ctcgcctttt tcattaccgg cagtgaaacc agccatcagc
    14761 accatgtggt cagccggaat gcggcttgcc gcaatacggc gggtggattc agcaatatca
    14821 acggtagatt cgaggtaatg acccactgcc agcagttttt cgaccggatc gataacggta
    14881 acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca
    14941 cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact gatgccatgc
    15001 aggacatgtt ttatttgggc aaattcctgg tcgacgaaag ttttcaattg tgccagcgga
    15061 aatcccggct gggcggcggc gagtcccgtc agaagttcgg caaaaatacg ttcggcatcg
    15121 ctgatattgg gtaaagcatc ctggccgcta atagtttttt caatcatcgc taccagatgg
    15181 ttggtaattt tggcgggggc agagaggacg gtcgccacct gcccctgcct ggcattgctt
    15241 tccagaatat cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac
    15301 ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt
    15361 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
    15421 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
    15481 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
    15541 taaaatttta atttgatcac ggcaggtaac cagttcagaa gctgctatca gacactcttt
    15601 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaatcact
    15661 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
    15721 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
    15781 atgtcatctg ccactgccag agtcgtcagc aatgtcatgg ctcgttcgcg taaagcttgc
    15841 agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat
    15901 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
    15961 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
    16021 cgaccaaaca ccagagcggc atggctcatc catgaagatt tttcctctaa cagcggcacc
    16081 agttctactg gcgtagcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca
    16141 gtgaaatcga catcgtgtaa cgattcagct agtgtcttga aaactttaat attatcaata
    16201 atatcaccag atccatgtgc gacccaacgg gcggctggct ccaggtacgc ctgactatcg
    16261 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
    16321 tctgctctgg cgggtgcaac cagaataatc gttatacgca tattgccact cttcttgacc
    16381 aaataaccgc gaacagggtg atcaccgtca acttattacg cggtgagaat ttacaaattc
    16441 ttaacgtaag tcgcagaaaa
//
LOCUS       DAJOTB010000108        16303 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJOTB010000108 DAJOTB010000000
VERSION     DAJOTB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.108"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(134..510)
                     /gene="rnpB"
                     /locus_tag="OHN28_003408"
     ncRNA           complement(134..510)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OHN28_003408"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(543..1688)
                     /gene="garK"
                     /locus_tag="OHN28_003409"
     CDS             complement(543..1688)
                     /gene="garK"
                     /locus_tag="OHN28_003409"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCQ2298741.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1785..2675)
                     /gene="garR"
                     /locus_tag="OHN28_003410"
     CDS             complement(1785..2675)
                     /gene="garR"
                     /locus_tag="OHN28_003410"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2298742.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2705..3475)
                     /gene="garL"
                     /locus_tag="OHN28_003411"
     CDS             complement(2705..3475)
                     /gene="garL"
                     /locus_tag="OHN28_003411"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCQ2298743.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3491..4825)
                     /gene="garP"
                     /locus_tag="OHN28_003412"
     CDS             complement(3491..4825)
                     /gene="garP"
                     /locus_tag="OHN28_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCQ2298744.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5200..6771
                     /gene="garD"
                     /locus_tag="OHN28_003413"
     CDS             5200..6771
                     /gene="garD"
                     /locus_tag="OHN28_003413"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCQ2298745.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6920..7255
                     /gene="prlF"
                     /locus_tag="OHN28_003414"
     CDS             6920..7255
                     /gene="prlF"
                     /locus_tag="OHN28_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCQ2298746.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7255..7719
                     /gene="yhaV"
                     /locus_tag="OHN28_003415"
     CDS             7255..7719
                     /gene="yhaV"
                     /locus_tag="OHN28_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCQ2298747.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7774..8583)
                     /gene="agaR"
                     /locus_tag="OHN28_003416"
     CDS             complement(7774..8583)
                     /gene="agaR"
                     /locus_tag="OHN28_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCQ2298748.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8832..10112
                     /gene="kbaZ"
                     /locus_tag="OHN28_003417"
     CDS             8832..10112
                     /gene="kbaZ"
                     /locus_tag="OHN28_003417"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCQ2298749.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10135..10608
                     /gene="agaV"
                     /locus_tag="OHN28_003418"
     CDS             10135..10608
                     /gene="agaV"
                     /locus_tag="OHN28_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2298750.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10619..11398
                     /locus_tag="OHN28_003419"
     CDS             10619..11398
                     /locus_tag="OHN28_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCQ2298751.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11388..12266
                     /locus_tag="OHN28_003420"
     CDS             11388..12266
                     /locus_tag="OHN28_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCQ2298752.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12284..12718
                     /locus_tag="OHN28_003421"
     CDS             12284..12718
                     /locus_tag="OHN28_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2298753.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12715..13848
                     /gene="nagA"
                     /locus_tag="OHN28_003422"
     CDS             12715..13848
                     /gene="nagA"
                     /locus_tag="OHN28_003422"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2298754.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14198..15352
                     /locus_tag="OHN28_003423"
     CDS             14198..15352
                     /locus_tag="OHN28_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCQ2298755.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            15365..16225
                     /gene="kbaY"
                     /locus_tag="OHN28_003424"
     CDS             15365..16225
                     /gene="kbaY"
                     /locus_tag="OHN28_003424"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCQ2298756.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3923 a         4337 c         4186 g         3857 t
ORIGIN      
        1 gtataattac gaccttttaa atgcaatata gacttaataa cttcttacaa agtctgttat
       61 taataccgta gatattcatc ttaccctggg cacacaaagc aaaaacccgc cgaagcgggt
      121 ttttacgtaa atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca
      181 tctaggccag caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac
      241 cttatgaacc cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc
      301 agccgcgcgg tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttacgcgggc
      361 catcggcggt ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc
      421 accctgccct atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga
      481 cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct
      541 tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg tgaagcacgg
      601 cagatattgt cataagctcc gcggagtgct tcgtctaacg taccaatgct ggtcagtacg
      661 ctgaagaccg catcaatgcc atgctgatgt acaacgccaa catcattggt caggctaccc
      721 gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcgacacc aatcggtacc
      781 ttgccgtgaa tactctggct atcaatacgc ccttcaccag tgatcaccag cgtacaatcg
      841 tgaatatgtt cctccagatt cagcgccgta gtgacgattt caataccact tttcagttct
      901 gcaccaagaa acgccatcag cgccgcgccc atacctcccg cagctcctgc gccggggaca
      961 tctttcacat caacatgcag cgctttttta ataacatcgg catagtgaga gaggttattg
     1021 tccagctcga caatcatcgc ctcactggct cccttttgtg ggccaaagat gcgcgaagca
     1081 ccactgtcgc cgactaacgg gttggtgaca tcacaagcga cgcggatgac acaatctttt
     1141 aagcgcggat cgagaccgga aatatcaata tcattcagag tattaagact accgccgcca
     1201 aaaccaattt cattgccgtt ggcatcgcat aatttcgccc ccagcgcctg taccatgcct
     1261 gcgccgccgt cgttggtggc gctgccgcca atgccgataa taatgtttgt cgcaccgctc
     1321 tccagcgcct gcaggattaa ctcaccagta ccgcgtgaag tggtcacgag tggatcgcgt
     1381 ttttccgcag gtaccagttc cagcccactg gccgccgcca tttcgataaa cgcggttttg
     1441 ccatcgccgg agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag
     1501 gcgtgacgtt cagccccctg ggtggctgca atcatcgctt ccaccgttcc ttcaccaccg
     1561 tcggcaaccg gaacagaaac atactgtgca tcaggaaaaa tttcccgaaa tcctttttct
     1621 atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg
     1681 attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg
     1741 gattccaggc ccggattgca atacgccatc cgggcacgac gtcattagcg agtaacttcg
     1801 actttcgcca gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca
     1861 tctgcacgca gtgcctgcat catctccata accgcagcag tgagcggcag ttgcgcgccg
     1921 acgccgtgag aagtatccag cgcattcgcc agatccttaa tatgcagatc aatacggaag
     1981 cccggtttga agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc
     2041 gccagcccac cgcgaattgc ctgatacacc agatccgggt taacgcccgc tttggttgcc
     2101 agcgttaacg cttctgacat cgcggcaata ttcagcgcca caatgacctg atttgccagt
     2161 ttggtgacgt tacctgcacc gatttccccg gtatgcacca cggaacccgc catcgctttc
     2221 atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta
     2281 ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc
     2341 gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc
     2401 aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc
     2461 acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta
     2521 gccgtagcgg ctgtttctgc accggcagca atcacgtcag caatagcttc tgggttacgg
     2581 tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata
     2641 atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg
     2701 gtggttattt tttaaaggta tcagccagtt tctgagtggc agagcggaag acgccgagat
     2761 cgctgccgac ggccacaaac gtcgcgcccc attccagata acgacgcgca tcagcctcga
     2821 ccggtgcgag gatgccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa
     2881 ttgctttttg tacatccggg tgcgaggcat tgccgagatg gcctaatgcc gcagccagat
     2941 cgctggggcc gacgaagatg ccgtctacac cttcggtagc ggcaatagca tcgacgttat
     3001 ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga
     3061 aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc
     3121 cttccggtgg gtaacgagtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa
     3181 aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt
     3241 tggtcggcac tcgcactact ggcgcgctgg cgctgccttt cagggccatt aactgcggaa
     3301 taaacgtgga gatatcgttt ggcgcatgtt caccatccag caccagccag tcaaacccag
     3361 ccaaaccaag aacttcagtg ctaatcgggt tagagagagc tgaccagcaa ccaatttgta
     3421 cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt
     3481 atacctttgc ttatttctgc aattccatac gtttaatgtc gccgactacg aagaggtagc
     3541 agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca
     3601 gttcacttac cagatagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga
     3661 cgttaaagac gccgccgcag aggccaacaa tctctttcgg cgcggtgtca gaaatcactg
     3721 gccagcccag cgcaccaaat cctttgccaa agaaagccag cgccatcagc atgaccacca
     3781 gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca
     3841 caattggtag cttacgtgcc agggtcaggg acaaaccgcg tttgatcaga taatccgaga
     3901 agacacctcc cagcacgccg cccgcaaagc cacacagtgc cggaatcgag gcgaccagac
     3961 ccactttcag aatcgacatg cctttttcct gcaccagata aattgggaac caggtgagga
     4021 agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccagc atcatgcggt
     4081 tagagagcaa ttgcttgatg taatgcagtt tgggtccgct tgctgccgca ctacccggct
     4141 ttttgtggtc catatcgacc accgcgccat tttccgagat aaacttcagc tcttctgcag
     4201 acatacgtgg gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcaacacaa
     4261 aaccaatcac ccccataacg gtaaagacat gctcccagcc ccaggcgaaa gtcagccagc
     4321 caagcagcgg cgaaaagagc gccaacgaga aatattgcgc cgagttaaag atggcggagg
     4381 cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg
     4441 atggcgcttc cgagaacccc agcataaatc gcataaagaa catggagatc ccagcccagg
     4501 ccagcggaaa catatcgaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc
     4561 tgtaagtgta aactttcttc gagccaaact tatcaagcag ccagccgccg ggaatttgca
     4621 tcagcaagta ggcccagcca aaagcagaga agatgtaacc catcgaaacc gcacttaact
     4681 gcaactcttt tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa
     4741 cggcggtaac aataaaaata atcagtaata ttaaatagcg ggtatgcacg cctttctttt
     4801 ttacgtcaac ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt
     4861 attatgggga aggtgttatt tatgagtttt atttatgccg ttacgacaat gaactcggga
     4921 attagtataa gcagcgcgag aataataatc attgtgcaaa tgctaattta attagtacta
     4981 tttaaatatt attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct
     5041 ttaccttgcc gggagatact gcaatccctg aaatgattga cattgatcac atttctgcat
     5101 ttaaactcct gacattctta tttcacccaa tgaagttatt tatttttaaa tgagaccagg
     5161 tcctcatttt aatagtccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga
     5221 caagaaacgc caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt
     5281 gttaatgata atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa
     5341 catattcccc aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt
     5401 cgttatggcg aggtgattgg ttacgccgtg cgcgcaatcc cacgcggaag ctggatcgac
     5461 gaatcaatgg ttgtactacc ggaagcgccg ccgttacata cgctgccact ggcaaccaaa
     5521 gtcccggaac ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat
     5581 ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc
     5641 gtggtggact acgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc
     5701 gatggcgtgg tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcacccgcg
     5761 gcagttgtac ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa
     5821 gtaatggtga ttggtctggg ttgtgaaaag ttgcagcctg aacgcctact gactggaacg
     5881 gatgatgtgc aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag
     5941 catgtcggtt ttcagtccat ggtcgaggat attttgcagg tagccgaacg ccatctacaa
     6001 aaactgaatc aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcag
     6061 tgcggtggca gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg gtatgcgtct
     6121 gatctattgg tgcgctgcgg cgcaacggtg atgttttcag aagtcacaga agtgcgtgac
     6181 gcgatccatc tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag
     6241 gagatggagt ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct
     6301 tcgccgggca acaagaaagg cggtctggca aacgtggtag agaaggcact cggctccatt
     6361 gctaaatcgg gtaaaagtgc aattgttgaa gtgctgtcgc ccggccaacg cccgactaaa
     6421 cgcggattaa tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg
     6481 gcttcgggta tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggccttatg
     6541 gcggtacccg tcattaaaat ggcgacccgc accgagctgg cgaaccgctg gtttgattta
     6601 atggatatta acgcgggcac tatcgctacc ggcgaagaaa ccattgaaga agtgggctgg
     6661 aagttgttcc actttattct cgacgtcgcc agcgggaaga agaaaacttt ctcggatcaa
     6721 tggggactgc ataaccagct ggcggtgttt aacccggcac cggtgacctg atttcttttg
     6781 gaacgtccct cgcaaaacat ggccttagtg ccatgttttt attgtttaaa gtccacacat
     6841 ccattaataa tgcatttgca ttaccttagt tcaagcttat aatttgagca gaaaacaggc
     6901 tgtaaaagga cagtaaataa tgcccgctaa tgctcgctct aacgctgtac tgaccactga
     6961 atcaaaggtc actatacgcg gacaaacaac tatccccgcg ccagtgcgtg aggccttaaa
     7021 actgaagcca ggcctggaca gcattcatta cgaaattctg cctggtgggc aagtatttat
     7081 gtgccgactg ggagatgaac aggaggatca tactatgaat gcatttttgc gttttctgga
     7141 tgcagatatc cagaacaatc cgcaaaaaac tcgtccattc gacattcaac aaggaaagaa
     7201 acttgtcgct ggcatggacg tcaacattga cgatgagatt ggcgacgacg aataatggat
     7261 tttccacaaa gggttaatgg ttgggcgcta tatgctcatc cctgttttca ggaaacctac
     7321 gatgctttag ttgccgaagt cgaggcatta aagggaaaag atcctgaaaa ttatcagaga
     7381 aaagctgcca caaagttatt ggcggtggtc cataaagtga ttgaggagca tatcacggtc
     7441 aatccatcat caccggcatt ccgtcatggc aagtcgttag gctctgggaa aaataaagac
     7501 tggtcacggg taaaatttgg tgctggtcgc tatcgtctct tctttcgtta cagtgaaaaa
     7561 gagaaagtca tcattctggg atggatgaac gatgaaaaca cgctgcgcac ctacggtaaa
     7621 aaaacagatg cctataccgt attcagtaaa atgttaaaaa gaggacatcc tcctgccgac
     7681 tgggaaagcc tcacccaaga gacagaagaa aaccattgat ggtgttcaca atgcccgccg
     7741 aacgcattgg tgttgaccag cgggcatcct ttctcattcc ccgaccagaa tcacttcaac
     7801 cccagccttt cgcagtcctt ccagactatc cgcaggaatg ccttcatcaa caatgatcat
     7861 gtcgatacgt tgagtatcaa tgatcttatg taaactggaa cggttgaact tactggaatc
     7921 ggtgaccacg atgatccgtt ccgcaacttc gcacatccga cggtttaaac gggcttcatc
     7981 ttcattatgc gtgctgacgc cacgctccag atcgatcgca tctacaccaa gaaacagcat
     8041 atcgaagtgg taattttgca gcgattgctc agcctgatca ccgtaaaaag attgcgactg
     8101 acggcgcaaa tgccccccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt
     8161 agccacgttc ataccgttgg tcatcgcaat tacgtcggtg tgcttgcgca tcagacgagc
     8221 aatctcaaaa gtggtggtcc cggagtcgag gatcacccga tgaccaggct gaatcaactc
     8281 aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac
     8341 tgatggctcg actgacggcg ttgtgctatc gcagatcaac gcgccaccat aggcacgcac
     8401 ggcgatcccc tgcttttcca gaaacgccag atcgttgcgg atcgtcacag tagatacgcc
     8461 atacaatgcc gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat
     8521 ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact
     8581 catagtaagt cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct
     8641 attaagcaaa tgcaagccca ccttacccat tggcgcaagc tactctcgtt tcagtggctt
     8701 tcattatgtt tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc
     8761 tcaccatgac gcagtatcaa ctgaaacaaa acgaaagatt aatgtcgcag taatctgaac
     8821 tggagaggaa agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa
     8881 atgggattta tgccgtttgt tccgcacatc cgctggtgct ggaagctgca atccgctacg
     8941 ccagtgcaaa ccaaacgccg ttactgattg aagctacctc caatcaggtg gaccagtttg
     9001 gcggttatac cggaatgacg cccgccggtt ttcgcggctt tgtttgtcag ctcgccgact
     9061 cgttgaattt cccgcaggat gcgctgattc tgggtggcga ccatctgggg ccaaaccgct
     9121 ggcaaaatct gcctgccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg
     9181 tggcggcagg attcaaaaaa attcaccttg attgcagcat gtcctgtcag gacgatccga
     9241 ttcccttaac cgatggcatc gtggccgaac gcgccgctcg tttggcgaaa gtggcggaag
     9301 aaacctgccg tgaacatttt ggcgaagccg atctggagta tgtcattggt accgaagtgc
     9361 cggtgcctgg cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg
     9421 cccgcgccac gctggaagcc catcgtcacg cttttgaaaa gcagggtctg aatgccatct
     9481 ggccacgcat cattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta
     9541 ttgattatca gcccgccaaa gcgaccgcct taagccagat ggtcgaaaac tacgaaacgc
     9601 tgattttcga agcgcactct accgattatc aaacgccgca atcgctgcgc cagctggtga
     9661 ttgaccactt tgccattctg aaagttggcc cggcgctgac ctttgctctg cgtgaagctc
     9721 tgttctctct ggcagcgatt gaagaagaac tagtgccagc aaaagcctgt tctggtctgc
     9781 gtcaggtgct ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg
     9841 gtgacggcaa cgcgcgtcgt ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt
     9901 actggccgga cagccagatt gatgacgctt tcgctcatct ggttcgtaat ctggcggatt
     9961 caccaattcc gctgccgctg atcagtcagt atctgccgct gcagtacgtg aaagttcgct
    10021 ccggcgagct gcagccaacg ccacgggaac tcattatcaa ccatattcag gacatcctgg
    10081 cgcagtacca cacagcctgt gaaggccaat aagcaaaaca aagaggaaca cgctatgcca
    10141 aatattgttt taagtcggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg
    10201 gtaggatttg cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg
    10261 gtacaacaaa acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg
    10321 ctgcaaaaag tcatcgacaa catccatcgc gccgccgatc gacagaaaat cctgctggtt
    10381 tgtaaaacac cggccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt
    10441 aacgttggca atatgcacta cgccaatggc aagcaacaaa tcgccaaaac ggtttctgtg
    10501 gatgcgggtg atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt
    10561 cagggcgtcc cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat
    10621 ggaaatcagt ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctgga
    10681 tatgtttaac ggcttaaccc atatgcaccg cccggtggtt ctcggcccgt tggtcgggct
    10741 ggtacttggc gatctgcata ccggtatttt aaccggcggt acgctggaac tggtgtggat
    10801 ggggctggcc ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg
    10861 cacggcgttt gccattacta ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc
    10921 tttcgctgtt gcagtacaga tggggattac cttcctgttc tcggtgatgt ccggcgtgat
    10981 gtcccgctgc gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtgaacta
    11041 tctggcactg ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt
    11101 cggcgcggaa catgcaaaaa ccatcattga tgtcctgccg caacgattaa ttgatggcct
    11161 cggcgtcgcg ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat
    11221 gaaaaacgtc tacatcccct acttcatcct gggctttgtt gccgcagcct ggctcaagtt
    11281 accggtgctg gctattgctg ccgctgcgct ggcgatggcg ctgatcgacc tgctgcgtaa
    11341 atctcctgaa ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc
    11401 aaaccaccct gccgaacgtc agcgaaaatg aagaaacact gctgactggc gtcaatgaaa
    11461 acgtgtatga agatcagagc attggcgcag agctgacgaa aaaagatatc aaccgcgtcg
    11521 cctggcgctc catgctgtta caagcgtctt tcaactacga acgtatgcag gcttccggct
    11581 ggttgtacgg tctgctgcct gctctgaaaa agatccacac taataaacgg gacctggcgc
    11641 gcgccatgaa ggggcatatg ggtttcttca atacccatcc gtttctggtg acgtttgtta
    11701 tcggcattat ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca
    11761 aaattgccgt cggtgcgccg ctcggcggga ttggcgatgc gatgttctgg ctaacgctac
    11821 tgccgatttg tggcgggata ggcgcaagcc tcgcgctaca aggctctatt cttggcgctg
    11881 tcgtctttat tgtgctgttc aacgtggtgc acctggggct gcgttttggt ctggcgcatt
    11941 atgcttaccg catgggcgtg gcggctattc cactcattaa agcaaatacc aaaaaagtcg
    12001 gccatgcggc gtctatcgtt gggatgacgg tgatcggcgc gctggtggca acctatgttc
    12061 gtttaagcac cacgctggaa atcacggcgg gcgacgcagt ggttaagtta caggctgatg
    12121 ttatcgacaa actgatgcct gccttcttac cgctggtcta caccctgacc atgttctggc
    12181 tggtacgccg cggctggagt ccgctgcgcc tgattgcagt gacagtggtt ctcggcatcg
    12241 tcggtaaatt ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt
    12301 gacagggcat ggcggatttg ccagcggcat ggaaaaagca atgaagcaaa tcctcggcga
    12361 gcaatcgcag tttatcgcca tcgattttcc agaaacctca agcaccgcgc tgcttacatc
    12421 gcagcttgaa gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga
    12481 tttgctgggc ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg
    12541 tgaagtgatc accggcacaa atttgcaact attactggaa atggtgctgg aacgcgaagg
    12601 gttaagcggt gaagagttcc gtgtgcaggc gctggagtgt ggacaccgtg ggctaaccag
    12661 tctggtggac gaacttggtc gctgccatga ggaatgtccg gtcgaggaag gaatatgaca
    12721 cacgttctgc gcgccagaag gctgctgact gaagagggat ggctcgatga ccatcagttg
    12781 cgtattgctg acggtgtaat cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc
    12841 gatgcggaac tgctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt
    12901 gatgtaatgg atgacgcgcc ggatgcgctc gacaagctgg caatgcacaa ggcacgcgaa
    12961 ggtgtcggca gttggttacc gaccaccgta accgcgccgc ttaataccat tcatgcggcg
    13021 ctgaaacgta ttgctctacg ctgccagcgc ggtgggcctg gtgcgcaagt gctggggagt
    13081 tatctcgaag gaccgtactt cacgccgcag aataaaggcg cacatccgcc ggagctgttt
    13141 cgcgagctgg aaattgccga gctggatcaa ttgattgccg tttctcagca gaccttacgc
    13201 gtggtagcgc tggcaccaga aaaagaaggg gcattgcagg ccatccgcca tcttaaacag
    13261 caaaacgtac gggtgatgct ggggcatagc gcggcgacct ggcaacaaac ccgtgccgcg
    13321 tttgatgctg gtgccgacgg cctggtgcat tgctataacg ggatgacagg tttacatcac
    13381 cgcgaaccgg gaatggttgg cgcgggatta acggacaagc gcgcctggct ggaactgata
    13441 gccgatggtc atcatgtaca tccggcggca atgtcgctct gttgttgctg tgcgaaagag
    13501 aggatcgtac tgatcaccga cgcgatgcag gcagccggga tgccggatgg tcactatacg
    13561 ttatgtggtg aagaagtgca gatgcacggt ggcattgtcc gtaccgcgtt cggtgggctg
    13621 gcgggcagta cgctgtctgt cgatgcggca gtgcgcaaca tggtcgagtt gacgggcgta
    13681 acgcctgcgg aagccatcca tatggcgtcg ctgcatccgg cgcgaatgct gggtgttgat
    13741 ggtgttctgg gatcgcttaa accgggcaaa cgcgccagcg tcgttgcgct ggatagcggg
    13801 ctgcatgtgc aacaaatctg gattcagggt caattagctt cgttttgatg gtttgctcct
    13861 ttattaggcc ttcacttccc ccgtaaggcc tttctttttc tttctttttg atctgtgcag
    13921 cggtgtcgga tgcgacgcta atgcgcctta tccgacctac ggttggtgac cgtaaggccg
    13981 gataaagtat ttacgccgca tccagcaatc ccttttactt cccttgtctt ttctttcaac
    14041 gatcacagat ttcgttttat ttcctttttc tccattgaac tttcagtttc ttttctatag
    14101 attttaacca acgaaagaaa tcaccaagtg aaatgaaacg aaaggcaagc gaaagcgaca
    14161 acgccgacgt caagttcatc agactaagga ttgagttatg ccagaaaatt acacccctgc
    14221 tgctgccgca accggtacat ggactgaaga agagatccgc catcagcctc gcgcatggat
    14281 ccgttcactc accaacattg acgcgctacg ttccgcgctc aataacttcc ttgaaccgtt
    14341 actgcgcaaa gagaatctgc aaatcatcct gaccggagcc ggaacatcgg catttatcgg
    14401 tgacatcatc gcgccgtggc tcgccagcca taccggtaaa aacttcagcg ccgtaccaac
    14461 caccgatctg gtcaccaatc caatggacta cctgaaccca gctcatccgc tactgttgat
    14521 ctccttcggt cgctccggca acagcccgga aagcgtcgcc gccgtggaac tggcaaatca
    14581 atttgtacca gaatgctatc acctgccgat cacctgcaac gaagcgggcg ctctttacca
    14641 aaacgcgatc aacagcgaca acgcgtttgc cctgctgatg cccgcagaaa cgcacgatcg
    14701 cggctttgcg atgaccagca gcattaccac catgatggcc agctgcctcg cggttttcgc
    14761 acctgagacg atcaacagcc aaaccttccg cgacgtggcg gatcgttgcc aggcgatcct
    14821 gacctcactg ggcgatttca gcgaaggtgt gtttggttac gcaccgtgga aacggatcgt
    14881 ttatctcggc agcggtggct tacagggcgc agcacgcgag tcggcgctga aagtgctgga
    14941 actgacagcg ggtaaactgg cggccttcta tgattccccg accggattcc gtcatggccc
    15001 gaaatcgctg gtcgatgacg aaacgctggt agtggtgttt gtctccagtc acccttacac
    15061 ccgtcagtac gatcttgacc tgctggcaga actccgccgt gacaaccagg caatgcgcgt
    15121 gatcgccatc accgctgaaa gcaacgacgt tatcgccgct ggcccgcata tcatcctgcc
    15181 gccgtcccgt cactttatcg acgttgaaca agcattttgc ttcctgatgt acgcccagac
    15241 gtttgcactg atgcagtcgc tgcacatggg caatacgccg gataccccat cagccagtgg
    15301 caccgtaaac cgcgtggtgc aaggcgtaac tattcatccg tggcaggcat aagaggatcg
    15361 cattatgagc attatctcca ccaaatatct gttacaggac gcccaggcca atggctacgc
    15421 ggtgcctgcc tttaacatcc ataacgccga gacgatccag gcgatcctcg aagtgtgcag
    15481 tgaaatgcga tcgccggtga tcctcgccgg gacaccaggt acctttaagc atatcgcgct
    15541 ggaagagatc tacgccctgt gtagcgccta ttccacgacc tataacatgc cgctggcact
    15601 gcatctcgac caccacgaat cgctggatga tattcgccgt aaagtccacg caggcgtgcg
    15661 cagcgcgatg atcgacggca gccacttccc gtttgccgag aacgtgaagc tggtgaaatc
    15721 ggttgtcgac ttctgccact cccgggattg cagcgtggaa gcagaactgg gccgcctcgg
    15781 cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca ttcctgaccg atccacaaga
    15841 agctaaacgc tttgtcgaac tgactggcgt cgacagcctg gcggtagcga ttggtacggc
    15901 gcacggctta tacagcaaaa cgccgaagat tgatttccag cggctggcgg aaattcgtga
    15961 agtggtggat gttcctctgg tgctgcatgg tgccagcgat gtcccggatg aatttgtccg
    16021 tcgcactatt gaactcggtg taacaaaagt gaacgttgcc acagaattaa aaatagcctt
    16081 cgctggcgcg gttaaagcct ggtttgcgga aaatccgcag ggtaatgatc ctcgttatta
    16141 tatgcgcgtc ggaatggatg cgatgaaaga agtggtcaga aataaaatta atgtctgtgg
    16201 ttcagcgaat cgaatttcag cataatcatt aattttatac gttttttcag aatgcactgc
    16261 ccgattaata tcgggtagta ccttaatggc aaaggagata acc
//
LOCUS       DAJOTB010000109        16296 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJOTB010000109 DAJOTB010000000
VERSION     DAJOTB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.109"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(225..761)
                     /gene="yghD"
                     /locus_tag="OHN28_003425"
     CDS             complement(225..761)
                     /gene="yghD"
                     /locus_tag="OHN28_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCQ2298757.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLNALDEKYALRVTQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            complement(763..1941)
                     /gene="gspL"
                     /locus_tag="OHN28_003426"
     CDS             complement(763..1941)
                     /gene="gspL"
                     /locus_tag="OHN28_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCQ2298758.1"
                     /translation="MSSILEIFFPLCDSDTVRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPFSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1938..2915)
                     /gene="gspK"
                     /locus_tag="OHN28_003427"
     CDS             complement(1938..2915)
                     /gene="gspK"
                     /locus_tag="OHN28_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCQ2298759.1"
                     /translation="MITSPPKRGMALVVVLVLLAVIMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2912..3517)
                     /gene="gspJ"
                     /locus_tag="OHN28_003428"
     CDS             complement(2912..3517)
                     /gene="gspJ"
                     /locus_tag="OHN28_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCQ2298760.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3514..3885)
                     /gene="gspI"
                     /locus_tag="OHN28_003429"
     CDS             complement(3514..3885)
                     /gene="gspI"
                     /locus_tag="OHN28_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCQ2298761.1"
                     /translation="MKRGFTLLEVMIALAIFALAATAVLQIASGALNNQQVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(3882..4445)
                     /gene="gspH"
                     /locus_tag="OHN28_003430"
     CDS             complement(3882..4445)
                     /gene="gspH"
                     /locus_tag="OHN28_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCQ2298762.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQQEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERVP"
     gene            complement(4449..4904)
                     /gene="gspG"
                     /locus_tag="OHN28_003431"
     CDS             complement(4449..4904)
                     /gene="gspG"
                     /locus_tag="OHN28_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCQ2298763.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4921..6144)
                     /gene="gspF"
                     /locus_tag="OHN28_003432"
     CDS             complement(4921..6144)
                     /gene="gspF"
                     /locus_tag="OHN28_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCQ2298764.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVVMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6144..7637)
                     /gene="gspE"
                     /locus_tag="OHN28_003433"
     CDS             complement(6144..7637)
                     /gene="gspE"
                     /locus_tag="OHN28_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCQ2298765.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7637..9697)
                     /gene="gspD"
                     /locus_tag="OHN28_003434"
     CDS             complement(7637..9697)
                     /gene="gspD"
                     /locus_tag="OHN28_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2298766.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9727..10686)
                     /gene="gspC"
                     /locus_tag="OHN28_003435"
     CDS             complement(9727..10686)
                     /gene="gspC"
                     /locus_tag="OHN28_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2298767.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLSQRQSLNTDKEHLRKIARAM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSVNKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAIAEPVVSAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10704..11114)
                     /gene="gspS2"
                     /locus_tag="OHN28_003436"
     CDS             complement(10704..11114)
                     /gene="gspS2"
                     /locus_tag="OHN28_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HCQ2298768.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11180..11989)
                     /gene="pppA"
                     /locus_tag="OHN28_003437"
     CDS             complement(11180..11989)
                     /gene="pppA"
                     /locus_tag="OHN28_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HCQ2298769.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGMATLYLQALF"
     gene            complement(12153..>16296)
                     /gene="sslE"
                     /locus_tag="OHN28_003438"
     CDS             complement(12153..>16296)
                     /gene="sslE"
                     /locus_tag="OHN28_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HCQ2298770.1"
                     /translation="SLRAVDKVSFSLEDAQELANSENKKTNAISLVTSSDSCPADAEQ
                     LCLTFSSVVDRARFEKLYKQIDLATDNFSKLVNEEVENNAATDKAPSTHTSTIVPVTT
                     EGTKPDLNASFVSANAEQFYQYQPTEIILSEGQLVDSLGNGVAGVDYYTNSGRGVTDE
                     NGKFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSTTGQN
                     NTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGDQNVVLPNEFIEQFKTGQAKE
                     IDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYQSVSKFHVFHDSTN
                     FYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSLVE
                     PENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSWNGSVNKDGQCTL
                     SGDSDDMKHFMQNVLRYLSNDIWQPNTKSIMTVGTNLENVYFKKAGQVLGNSAPFAFH
                     EDFTGITVKQLTSYGDLNPEEIPLLILNGFEYVTQWSGDPYAVPLRADTSKPKLTQQD
                     VTDLIAYLNKGGSVLIMENVMSNLKEESASSFVRLLDAAGLSMALNKSVVNNDPQGYP
                     NRVRQRRATGIWVYERYPVVEGALPYTINSETGEVTWKYQIDNKPDKKPKLEVASWQE
                     EVDGEQVTQYAFIDEADYKTPELLAAAKKRILDAFPGLEECKDPDYHYEVNCLEYRPG
                     TGVPVTPENNTGMFVPRYTQLNLDADTAKAMVQAADLGTNIQRLYQHELYFRTKGSKG
                     ERLNSVDLERLYQNMSVWLWNDTKYRYEEGKEDELGFKTFTEFLNCYANDAYAGGTKC
                     SADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEK
                     YPGSVSAKGESVTENISLYSNPTKWFAGNMQSTGLWAPAQQDVTIKSSASVPVTVTVA
                     LADDLTGREKHEVALNRPPRVTKTYTLEANGEVTFKVPYGGLIYIKGNSSTNESASFT
                     FTGVVKAPFYKDGEWKNDLDSPAPLGELESASFVYTTPKKNLEASNFTGGVAEFAKDL
                     DTFASSMNDFYGRNDEDGKHRMFTYKALTGHKHRFANDVQISIGDAHSGYPVMNSSFS
                     TNSTTLPTTPLNDWLIWHEVGHNAAETPLNVPGATEVANNVLALYMQDRYLGKMNRVA
                     DDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDITKWYPDGKLPAFYSE
                     REGMKGWNLFQLMHRKARGDDVGNSTFGGKNYCAESNGNAADTLMLCASWVAQTDLSE
                     FFKKWNPGANAYQLPGAAEMSFEGGVSQSAYNTLASLKLPKPEQGPENMNKVTEHKMS
                     AE"
BASE COUNT         3601 a         4651 c         4114 g         3930 t
ORIGIN      
        1 aatgtgtcaa aacacttaag catctttaca gtcttagaaa ggtgtcgatt aacgaaggct
       61 acatggtgaa aaacaggttc cttcagtgtt accgaatatt ccgtggtggt ctggagggag
      121 ataattcttg ctacatcgaa actcagcccg gtgagcgggt ttgacagttc cgcactcacc
      181 attgggctaa gggttatcag gtggggttaa ggaaatggca atacctaccc ccgtccaaac
      241 tccagtcgct gcacattcac catcccaggc ttctcacctg cgctgacatc aatttgtgtc
      301 acccgcagcg catatttttc atccagcgcg tttaaccagt tcagcaggtc attaaacacc
      361 acaggttcta tccagacttg aatattctcc ccacgctcgg ctatccgctt gatgaccacc
      421 gagtgcgcgg aagcactgtc actgatgacc cgcgatacct gcgcaggcgt tgttgtggca
      481 gattttcgcg ctgcaataat atccggcgcg gcgttcttca gtcgggtgtt cattgccacc
      541 agctgttgca acatcgtctc ctgttgttct atccgttcgc tcaacggctg ccagatgagg
      601 acgtaatatc cggcactaaa caggaacact accgctgcca gtaacatgcc tttttcacgc
      661 ggagaacgcc ccgccaggtg ttgtgtcagc cagtgttcgc cacggcttaa ctggcgttca
      721 cgccattgct gaaaatagtg aataaattta tcgcgtaaca tgttatttcc tccgcaacgt
      781 tacgccgccg gaaaccgcat caccctcttt ctgtaatgcg tcctgttgca caacataatc
      841 tgccgccagt gcgctacgca gtttgtcgaa actggcaaag ttcgcggccc gcagctggag
      901 gtgaagcgtc tggcgttttt gatcaaaggt gaaaccacgc atttcgatgt cggtaagtga
      961 cgctgatttc agggtactgg cgatcgctga caattctgcg agcagccggg tatcgtcggc
     1021 ctgtgggcga tattttttca gcgccatcgt cacctgagaa cgcaaattca caatccgctt
     1081 ctgctccggg aacaacgtta agaactgttt ctccgcctgg gtgcggcttt gcgccacctg
     1141 ttcgctgacg ctccataacg tcacgccccg ttccactgcc agcgcaacca gaatcagcaa
     1201 tatcggcaga atcatcaccc gccagcgcgc ccactgtttt cggtagctga cgcgaggctg
     1261 ccacggtcca gtcagcaggt tcccttccgg ttcgccataa gtggtaatgg cgggcagagc
     1321 cgtcactgtc aggcgttcgg cgtctgcacc agcccatgca gacagttctt ccggtgcaat
     1381 gccgaccacg gttagtgaaa acggtaaatc ctgctcattg agctgtgcgc ggaacatgac
     1441 cggagccagc gcccgcccgg cgctccatcc ccggcattca tcgatgcggc agataacccg
     1501 ttgcgcatcg ccagccatat agccacaagg aatggacatc cagtctggcg cgacggtagc
     1561 gcgggtgatg ccgtttgcct gcaaccattg cgcaatgtta cgcatatgct gttggtgaat
     1621 cactgctacg gttgccagtt gctggtcgat tttcaacggg gcgaaatgca gttcatcgat
     1681 atcctgattc agctcttctt ccagcaaggc gggcagaata gtcggtatct gcttgcgggc
     1741 gacatcaggc agttcaacct gccagacgct gatccattcg ccgggaatgt agagtcgaat
     1801 cgcatcggtt tgcagccatt gctggagacg ttcgtcagca acgtcaggcc agataccgtg
     1861 ttccacatcg ggtgtacggc gctgccagcg gacggtgtcg gagtcgcaaa gcgggaaaaa
     1921 aatctcaagg atggaactca ctcactttct cctgtctgat gccagagaac agaaaagtgt
     1981 tgtgggccca tgcggacaat taacgaattc atcgtcagtt caatctcatt cacggtgata
     2041 tctgaacgca gccagaagta attgctgtcc acgctcagga cggtttttag ctgtttttta
     2101 gtacgctcat cgacgtctgc gagtagcggc tgtgcaagaa actgatcgac atcttcccag
     2161 cccttcgccg gacgttgttg taataacgcc cgcgcctgaa cagggcttaa ccacgggtca
     2221 aacagcgcct caagaatcac actttgcgtg acatctaatg tattgatgtt gatttgctgg
     2281 cgggccatcg gcagcgcaca gaccaacggt ttcagttttt gataaagccc ggcgtccatt
     2341 ccctgtacca cgcgcatttc gctgatatca gccagcggtt gattagcggc gtagaacggc
     2401 accgagcggg cgagatactc gctgtcttca cgacccagac gcgtctgcac gctgcggtct
     2461 tcgtcaataa actcccacag gctttcggct atcagttcgg cccgataagc aggcacatcg
     2521 aggcgcgaga tcagggcaat cagttgttgt accgcgagcg gacgcgacgc cgtcgtcggc
     2581 tgagcgaggg cattcaggtt aaagcaggcc tgtgcgtcac gcagagtgac ggcaatttgc
     2641 ccctgcggca gtgggaaaaa acgcgggcca gaagcccacg gttgtgccag atgcacacgc
     2701 ttttcatttt tcaggctcag actgagcgcg ctcagcgcca ggctttctgc actggcgctg
     2761 taccacagcg cctgctggta ctcctgctgg ctgcgcgttc gcccaagttg ttgctgcatc
     2821 cgcccggaaa gcgtgatggt taccagcatt atgaccgcca gcaataccag caccacgacc
     2881 agtgccattc cgcgttttgg tggtgaggtg atcatgataa ttgcggcccg cgtaacaacc
     2941 agatgcgttc aatttcgccc cattgtggcg aatgcagggt tatgcgtact gccacaggaa
     3001 tcgcctgcac tgatgaccag ctctcctgcc agcgcgtgcc gtcgtagaac tgcaaacgga
     3061 gcgaatccgc cgggattaat ttttgcgttg ttggcttcac gctgcctgcc gcatcggtca
     3121 gtggccaggc taaccgttcg agataaccac catgaatgcg gtaaccgacg gtgagcagat
     3181 tactgcgcgg cagacgcatc aacggattaa ccacgccgcc acgtacaaaa cgcatccctt
     3241 cactctcaga cgccagcacg ccagcgcccg ccagtaacgc tggttcacgc tggccctgat
     3301 cgcctcttac cggacgcggc atcatttggg tcagatcgtg gttcaaaaaa ctcatcgttt
     3361 gctgcatgag gtttagtttt tgatcgtgtc cggcgacggc gctattcacg cgtgtaacgc
     3421 cgtttgtcac ctgctgcgcc atcagtgcca gtgaggcaaa aatggctatc gccaccagca
     3481 tttccagtaa cgtgaaacca gcgcgagccc ttctcactgt tggcctccca cggcgctaaa
     3541 ccaggcgcgt cgtgactgaa tcactggcga aaagtcgtca tgaagactga cttcaatatc
     3601 caccgcctgg agcagcacgt taccggtatt cagagggatc gttcgccagt accagcggct
     3661 tcctgccata tcactctcgc cctggtgcct gaccgctcgt tgttcacggg tcatcaggta
     3721 aagcagcgca gtctggtttt cagctaccca gcccgctacc gttttctcct caagaacctg
     3781 ctgattgttt agcgcgccgc tggcgatctg taataccgcc gtggcagcca gcgcaaaaat
     3841 cgccagtgcg atcatcactt ccagcaacgt aaatccgcgc ttcatggcac cctctcatca
     3901 cattgattga gggataacgc gccatcgtgc gccagtttta ccgcccaaca tgcgttttgc
     3961 gccgccgaat aaaatcgcag cgtaaacggc gtggcgggtt caaaaggcga aaaacggatc
     4021 tgcggtgtct tctttttcgc ctcttgttgc agctccagtt cgatatcgtg cagcgtcagg
     4081 cgacgacgct gcagctccag cgcatattcc ttctgccaga tatcactgcc gggttgtaac
     4141 agcatctgca cctgtttcgg caccgtaacc tgtgccgata accgggtcgc agaaacgggc
     4201 aaccattgcc cgtgacgacg ctgcataaac tggtaacctg gcgggtcgat tagcacaccg
     4261 agtgtttgcc cttcgatcac tgccctgtcc ttaaactgcg caaagcgagt cagaaaatcc
     4321 tgcgccgctt ttttcgcagg cggctctgaa gcggtcgcaa acgtctgtac cacgcccgca
     4381 ctggcaaggc cgataaggaa aatcaccagc atgatttcca gaagtgtgaa tccgcgttca
     4441 ggcactgatt actgaaactc ctgcaaattc cagttaccga tatctgcgcc agcgccctcc
     4501 ccgttttcct gaccatctgc tcccagggta taaacatcaa acagcccttt ttcacctggg
     4561 ctgagatact gataatcatt gccccacgga tcctttggca gtcgtttgat gtatccaccg
     4621 gtacggtagt tgcgggcgtc cgccatattg gctggctgct ggatcagcgc ctccagtccc
     4681 tgttcggtgg tcggataacg cccgttatcc agtcggtaca tatccagcgc gttttccagc
     4741 gccacgatat cgctgatggc tttttgccga tcggctttct ctttgttgcc cagcaggtta
     4801 ggcaccacca gacttgccag gacgccaaga ataacaatca ccaccatcac ttccagcagg
     4861 gtaaaacctg cccgtggttt ttgtgtgcgg gataacgaat tcatgtgata actccgtaaa
     4921 ttacattcca accatattgt tcagttgcag catcggttcg aggatggcga tgacgataaa
     4981 cagcaccacg cccgccatca ccaccaccag cgccggctca aacagcccta acgccagacc
     5041 cacctgggta tcaaattccc gttcctggtt gaccgcggcc tgttcaagca tggtttccag
     5101 ctcgccgctc tgttcgccgg aggcgatcat gtacagcatc atcggcggga acaggcgcag
     5161 atccgccagc gcggcgcgca ggctgcttcc ttcgcggacg cgatctgccg ccagcagcag
     5221 ttgttgctcg acataacgat ttgccgacac ggcggcggcg gtctgaatgc cttccagcag
     5281 cggaacactg ctggcggtga ggatgcttaa cgtgcgggaa aaacgcgccg tattcagtcc
     5341 gcgcgcaaca cgccccgtca cgggcaaacg cagcaaggtt ttatcccagc gcaggcgcat
     5401 cgcaggattt ttgagtagcc gttgccccag caccagaagc cccagcaaac ccgccagcca
     5461 gtacacgccg ctggtctgta acgtgtcgct catggcgatg agcattcggg tggaggcagg
     5521 cagcgcgtgt ccgagatgat caaactgttc gataattttc ggcactactg ccgtcagcaa
     5581 aatagtgacc acgcccgttg ccaccaccag cagtaccagc gggtagagca tggcctgcaa
     5641 tagacgtgat ttcaggcgct gtcgctgttc ggtgtaatcc gccaggcgat tgagcaccac
     5701 gtcgagatgc ccggattttt ctccggcagc gaccatcgaa caaaacaggg agtcaaagac
     5761 gcggggatgt tcgcgcaggc tgtccgacag ggtataacct tcctgaatcc ggctgcgcag
     5821 cgccattccg aggcttttta catgcagttt ttcgctttgc tcactgaccg cctgtaagca
     5881 ggtttccagc ggcattgctg cctgcaccag cgttgccagt tgacgagtga acagcgccag
     5941 atctgccgcc gccacgcgac gatgtgcgtg ccgccgacgc tgcaacattc cccctgccga
     6001 tgcattcatc cgggcttcaa tgtgcacggg aatgaggtct ttaccgcgta acaattgacg
     6061 ggcatgacgc gcggaatccg cctcaatcat gcctttggtt ttgcgaccat tacgctccag
     6121 cgcctgatag taaaacagtg ccattacgcc tccatggtta cccgcagaac ttcatcgaga
     6181 gaggtttctc cggcgagcac tttctcaatg ccgttgctgc ggatacccgc agagtgttgc
     6241 cggacataac gttccagctc cagttccccg gcctgacggt ggatcaaatc acgcaatgtg
     6301 gcgtccacca cgatcagctc atggatggca gtacgtccgc gaaaaccttt atgattacag
     6361 gcgggacaac cctgcggatg gtacagcgtg acggttcgcg catcggtaat ccccagcagg
     6421 cgtttttctt cgtcggtggc aggcgcggac tggcggcaat cgggacacag cgtgcgaacc
     6481 agtcgctgcg ccatcacgcc cgtcagactg gaagagagca ggaaaggctc cacgcccata
     6541 tcctgcaaac gtgtgatcgc ccccaccgcc gtgttggtat gcagcgtgga aaggaccagg
     6601 tgtccggtca gtgaagcctg aacagcgatt tctgcggttt cggtatcgcg gatttcaccg
     6661 accatcacca catccgggtc ctgacgcaaa atcgcgcgca ggccacgggc gaatgtcatg
     6721 ccgacgcggg tgttaacctg cgtctgaccg atcccttcaa tcatgtattc gatagggtct
     6781 tcaaccgtga gaatgttacg cgagtggttg ttcagctcct gcaatccagc gtacagcgtg
     6841 gtgcttttgc cggaaccggt cggccccgtc accagaaaaa tgccgtgcgg tttgtgtaac
     6901 agctggcgca actgcgcagt cagttcgaga cttaaaccca gacgctccag cgtcaggcga
     6961 gcctggtttt tgtccagcag tcgcagcacc acccgttccc cccaggcgga aggcatggtt
     7021 gatacacgga cgtcaatcgc ccgaccgccc agcagcagcg caatacggcc atcctgcggt
     7081 acgcgctttt cggcgatatc cagtcgcgcc atcaccttga tacgcgagac cagcagcgag
     7141 gccagtttac gaccggggcg caacatttca tgtaatgtgc cgtcaacacg aaaacggatc
     7201 accagactct tttcaaacgt ctcaatgtgg atatccgaag cgccttcttt gattgcctct
     7261 gccagcatgg cgttgatcag tttgatgatc ggcgcatcgt cgtcactttc cagcagatct
     7321 tccgtttccg gcagttcttc agcgagggta aaaaagtcct cggcggaacc gagatcttcc
     7381 atcagctggc gagcttcgga agagtcccgc tgccagaccg cattcagccg ctgttcaaat
     7441 tcggcctcgt cgatttgccg cagcgtaaaa ggcgcgttca gcccccgctg tagctcctgt
     7501 aaaacagaga gcgacaacgg atgaacatgg aggatctcca gcgacgcttc gcaccatgcc
     7561 accaggctaa accgacggct gaaactgtag ggcagacgca cggtgttagc ggtggtttcc
     7621 tgtgctacag gcaccattaa cgcgttctcc cggcattgag gaacgcacga acttctggcg
     7681 gtaaggcctg attttgcgca ggcaatatcg gctgcgcggt gtgcggcatc aggcttaagc
     7741 cttgctcatc gcggtagatt tgctcagcgc gcatgtagtt atatttgcgc tgcgacacgc
     7801 cgtctgccgc cataccgtca cgcaaaatgg tcgggcggat aaacaccatc aggttacgtt
     7861 tttctttttt atccgccgtc gatttaaaca ggttaccaat caacgggata tcgcccagca
     7921 gcggcacttt cgccacgctt tctcccgcct ggtcgtccat cagaccgcca agcacgatca
     7981 gctcaccatc gttagccagc acggtggttt tcagtttgcg ctcgccaaac acgacatcga
     8041 ggctggtctg cccttccact ttcgagactt cctgctcaat caccatctgt accgcgttac
     8101 cttcgttaat ctgcggcgtg actttcagca tgatgccgac ttttttcctc tctaccgtat
     8161 tgaaaggatt gctgttattg gaaccaacgg tagagccggt taataccgga acgtcctggc
     8221 ccaccatgaa gaaggcttcc tggttgtcca gcgtggtgat actcggcgtg gagagcacgt
     8281 tcgagctgga gtcgtttttg accgcctgta ccagcgccat ccagtcgcct ttcaccacgc
     8341 caaccgccgt accactaaag ccagaaagga gctgagcgag cgtggagaga tcgccgttag
     8401 tatccggatt tatggtggta gcaccgtttt cgctgatcac cgtggaacct ttctgcggct
     8461 tggctgcaga aatcgctgcc cccagcgtac caatagggat ttgcgtaccg ttggcaaact
     8521 gcattaatcc agcatctttt gacccccact gcacgccgaa gttgatattg ctaccttcgg
     8581 caacttccac gattaacgcc tcgacatgca cctgagcacg gcgaatatcc agttgttcaa
     8641 tcacgctttg cagcgactgc ataatgtcct gcggcgcagt aacaatcagg gcattactgt
     8701 gtttgctggc ggcgatggag acaacctcgc gcccgctacc aaccgtgcct tccgcctctt
     8761 ctttagccgc cgtgagcgta ccgctgacct gcttcagcac atcgaccaga tcttcggctt
     8821 tgctgtattt gagatagaaa acctggctgt tgccgctgcg ttccatttct gagtccagcc
     8881 gacggatcag gcggcgcatt ttgtcccgcg tggccgggtc accactgaca atcacactgt
     8941 tggtgcgttc gtcggcgaca atttgagatt tcagcgtcgc aggctggttc tcgccgctgt
     9001 ttttcgtcag gctttccagc acgcgggcaa tttccgaggc tgaagcgtta tccagcggga
     9061 tcacctcttc ggtgcgatta cctgcgtgat ccacgcgctg gatcacttct gtcaggcgct
     9121 ccacaactga ggcgcgtccg gtaagcataa tcacgttgga gggatcgtaa ttaacaacgt
     9181 tgcctgagcc tgcgctgtca atcatctggc gcagaatcgg tgccagctcg cgtaccgaca
     9241 cattacgtac cggcacgact ttggtgacca tttcatcgcc cgcgtagttg tcgctgcctt
     9301 caccgaccag cggcagcggc tcgactttcg cagcgctgga tttcaccacc ttcagcacgt
     9361 cgttttccat cggtacgacg gcatacccct gtgcttccag caggttaagg aatagctggt
     9421 aatactggcg ttcattgagc ggggtcatgg tgcgaatact cactttcccc tgaacgcccg
     9481 gccccataat gatggtttta ttaaggttag cgccgacggt ttcgatgaac gatttcaggt
     9541 cggtatcttt aaaattagcg gtgaaagtgg cttcttccgc ccagaccggt gaactgcata
     9601 atgccgctgc cagcaccagc ggcagtaaac gcttttttgt tttgaggcca gttgtcttct
     9661 tacgccagac cgacaacgtc atatcacgcc aaaacacgat gaatgattct cctggattaa
     9721 atgcggttag cgcagcgcga tggaaatgtc gtagcgcgca cccttgcgta aaaccgtaag
     9781 ttgaatggaa tccattgaag gtaactgccg catcagagca atcattgctc gtggatcagt
     9841 gaaatcctgc tgatttagcg caatggcgat atcgccttcc ttaaaaccgc tggcatcgaa
     9901 cagagaacga tctgcccccg gtttcactgc ataaccgaca atcccctcct tacgcacagg
     9961 cgtaagctgg atatagttaa aaattttctg cggatctttc gccagtgcct gacgcacggc
    10021 agccgggatc tccactggcg cactgacgac aggttcagca atagcttgct ttgcttcgtc
    10081 actggcggct tttttgctgg tcaccgccac aggggggcgt tcctcctctg ccagactcag
    10141 acgttccatt ttcccctgat agcgcagcat cacatggtcg cggttgattt cctcaatcac
    10201 tgcgttgtga gagccaagcc gttcaccctg caaatagacc tgctgtttgc cgccttcttc
    10261 aatcaccgcg ccgggtctgg caccaaaggc gatcccacgc agcaccacat taagacgcgt
    10321 ttctgccaca ggcgcagatt caggttgttt taccggtgcg gcgacgggtt gatatttgcc
    10381 aaaccagttt tgttggctga ctaattgcac gtcattttta tcgaacggtt ttgcatccgc
    10441 acggagcggt ttattcaccg atgaggaaac cgccgtatat tccgcagaaa aggagaaata
    10501 gcgccagagt gaatacgcca tttttgcaga aataataagc agcatcagcc agaacatcgc
    10561 gcgtgcaatt ttgcgcagat gctctttgtc tgtattaaga gattgtctct ggctaagagt
    10621 gtgacgaatt ttagtcagcc attgaatgag ataaattcgt gcgtcacgaa aaacaacccg
    10681 cgccaattta tatctacccg acgttatgct ttgactattc cacaggtggt acgatccagt
    10741 ttccgctgat actggttacc tgtacgtgta tcattaatcg ttatgctgta attaattccc
    10801 tcggtgatca ttaatttcac cgttgggtca gcgcacattt gtcgttgata gctggttaaa
    10861 aaggcgtcag gtgtctgcgt ggtctgagta cccgattcgc tgataatggt catttttacc
    10921 gtcgtcccac tactttgcgc cagcactaag gtatatcccg cagatttaat cggcaggttt
    10981 tggctgatat tttgcgcctg ttttttcgcc agtaaactgg cgttttcatt atggctggcg
    11041 cagccactta ataatcctgt aacgctcaac aatagcgata ttaataccct ccctggcatc
    11101 tgttttatcg acatggtaaa taatctctaa ggttattaat aagagttaaa atgtcacttt
    11161 gataatgacg tggttatcat taaaacaatg cctgtagata aagtgttgcc ataccgccca
    11221 gacttaaaca cggtccaaaa ggcagtgtgg ttgatcctct ttttgtaata acggcatata
    11281 tcaggccgca gcatgaggcg attaaagcca cattgggtag cgacaacggc cccacccagc
    11341 cacctaacgc agcgaaaagt aatacatcgc ccatgcctaa tgcttcttta cgcagaacta
    11401 ttccggctat ccagcgcagg gagtaaaaag tgataaaccc caccaggacg ccggtgactg
    11461 cgtcttgtag cgttaacgga ctctgttgcg cccatgccgc aatcagcccg gtccacaata
    11521 cgccctgagt aaaaacatcg ggcagccatt ggttgtcgag gtcgatgatg ctcgcggcaa
    11581 tcagccacgc ggataatatc atcaccgcca gtccccatcc actttctggc cacaccagac
    11641 tcgccagcaa aaaagcgagt gctgtcaata attccaccag cggataacgc ttgctgattt
    11701 tcgcctgaca gtcgcggcag cgccctttga gcatcaacca tgagagcagc ggaatattgt
    11761 cacgaacgcg gatagtctgc tggcaatgcg gacagtgcga acgcggtagc gcaaggctta
    11821 tttttgactg cgtactcggc gtttcaccat gaaactccgc catttgttgg cgcagcatga
    11881 tggggtaacg ccaaatcacc acattcaaaa aactgccgat gatcaatcct ccgacggttg
    11941 ccagtatggg catcgccgcg gggtattgct gaaaaacatc aaaaagcatg gttaaaggtt
    12001 atttgttgta atttgccgga tgcgggcctg caggtgtatg ccatatggct ttcctccagg
    12061 cccgataaga tggcgcatcg ggcaaatgtg ttaacccgat gcgccttatt tcatgccgga
    12121 tgcggcgtga acgccttatc cggcctacag gcttactcgg cagacatctt atgctcggta
    12181 accttgttca tgttttccgg cccctgttcc ggtttcggca gcttgagcga cgcgagcgtg
    12241 ttgtaagccg actggctcac accgccctcg aagctcatct ctgccgctcc cggtagctgg
    12301 taagcattcg cacccggatt ccatttctta aagaactccg aaagatccgt ctgggcgacc
    12361 caggaggcac acagcatcag cgtgtcggca gcgttaccgt tggattcagc acagtaattc
    12421 ttgccaccaa aggtgctgtt accaacatca tcgccgcgtg ctttacgatg catcaactgg
    12481 aacaggttcc agcctttcat cccttcacgc tcgctgtaga acgctggcag cttgccatct
    12541 gggtaccact tcgtgatatc aaagtttttc tctgcccact ctttcaactg tgcgtacatc
    12601 agcagacggt cccccgcacc gccgcgcgcc caggcctgac cgttgctctc ctccagatat
    12661 tccggcgcga cggtaatgtc gtcagcgaca cggttcatct taccgagata gcgatcctgc
    12721 atgtacagcg ccagcacgtt gttcgccact tcagttgcac ccggtacgtt cagcggtgtt
    12781 tctgcagcgt tatgaccgac ttcgtgccaa atcagccagt cgttcagcgg cgtcgtcggc
    12841 agcgtggtgc tgttcgtcga gaagctgctg ttcattaccg gataaccaga gtgcgcatca
    12901 ccgatggaga tctgcacatc gttggcgaaa cgatgtttgt gacctgtcaa tgctttatag
    12961 gtaaacatcc ggtgcttacc gtcttcatca ttacgaccgt agaagtcatt cattgagctg
    13021 gcaaaggtgt ccagatcttt agcgaattct gctacaccac cagtgaaatt gctggcctca
    13081 aggttcttct tcggcgtggt atagacgaac gacgcagact ccagctcgcc cagcggcgcc
    13141 ggtgagtcca gatcgttttt ccattcgccg tctttataga acggcgcttt tactacaccg
    13201 gtaaaggtga agctggctga ttcattggta gaactattgc ccttgatata aatcagacca
    13261 ccataaggca ccttgaaggt cacttcaccg ttagcctcca gagtatacgt tttagtcact
    13321 cttggcggac ggttcagcgc aacttcatgc ttctcacgtc cggtcaggtc gtcagccagc
    13381 gccacggtaa cagtcactgg gactgacgcc gaagacttaa tggtgacgtc ctgctgggcc
    13441 ggtgcccaca ggccggttga ctgcatgtta cccgcaaacc atttggtcgg attcgagtac
    13501 aggctgatgt tttccgtaac gctctcaccc tttgccgata cggatcctgg gtacttctcc
    13561 acatcaacct taatgttcag atcccaccag gaacgaccca gcatcagacg cgtcagcggt
    13621 ttttccatat agttgagcgg atagctcggg ttcatcatgc ccgctttgcc gctactttca
    13681 ccgtagatca tgttgttatc gaccagcgat tttttcagat ctgcggagca cttggtgccg
    13741 cctgcatagg catcattggc gtagcagttc aggaactcgg tgaacgtttt aaagcccagc
    13801 tcatcttcct tgccctcttc gtaacgatat ttcgtatcgt tccacagcca gaccgacatg
    13861 ttctggtaca gacgttccag atcaacactg ttcagacgct cacctttact gcctttggta
    13921 cggaaataaa gctcatgttg atacaggcgc tgaatgttgg tgcctaaatc tgcagcctgc
    13981 accatcgctt tcgcagtgtc ggcgtcaaga ttcagttgcg tatagcgtgg aacaaacatg
    14041 ccagtattat tctctggcgt tacaggaacc cccgtgcctg gacggtattc gaggcagttg
    14101 acctcatagt gatagtcagg atccttacac tcttccagcc cagggaacgc atccagaatt
    14161 ctcttcttcg ccgcagccag caactcaggc gttttgtagt cggcttcatc aataaacgcg
    14221 tattgagtta cttgttcacc atcaacctct tcttgccaac tggcaacttc cagcttcggt
    14281 ttcttatcag gtttgttatc aatctgatat ttccaggtaa cttcgcctgt ctcagaatta
    14341 atggtgtacg gcagcgcacc ttccacaacc ggataacgtt cataaaccca aatgccagtc
    14401 gctcggcgct ggcgcacgcg gttcggataa ccctgcggat cgttgttcac caccgatttg
    14461 ttcagagcca ttgacagacc cgcggcatcc agcagacgca caaaactgga cgcgctctct
    14521 tccttaagat tgctcatcac gttttccatg atcagcaccg agccaccttt gttcagataa
    14581 gcgatcagat cggtcacatc ctgctgagtc agcttcggtt tgctggtatc tgcacgcaga
    14641 ggcacagcat agggatcgcc agaccactga gtcacatatt caaagccgtt gaggatcagc
    14701 aacggaatct cttccggatt cagatcgcca tagctggtca actgtttaac cgtgatacca
    14761 gtgaaatcct catggaaagc aaatggtgca ctatttccca atacctggcc cgcttttttg
    14821 aaataaacgt tctccaggtt ggtgccgaca gtcatgatgc tcttggtatt tggctgccag
    14881 atgtcatttg acaagtagcg caggacgttc tgcataaagt gcttcatgtc atcagaatca
    14941 ccgctgagcg tacactgccc gtctttatta acgctcccgt tccaactgta accattaggg
    15001 caacgcaaaa tgctgttgta gtgcgggtta ccgataacca tcagcttgcc ctcaccaact
    15061 tgccccagcg aaataaatgg caggttgaag gtggcggtat cgcgcgtaac gttttccggc
    15121 tcaaccagag aaggcgcttc cgtaatgtac gccagctcat ttttatccca ggcgcgtttt
    15181 tcgccaaacg ccagccagta gtttttatca ttacgcgcca tcagaatcgg gaaggcagcg
    15241 ttggagatat tcaccaccgc ctgaccgcgc gcattacccg tgctgccata gaagttggtg
    15301 gagtcatgga atacatggaa cttgctgaca gactgatagt tcgtatccac accccacagc
    15361 ttgttaataa cgccctgaat ctggccgtca ttaacattgc gcgttgtcag cgagaaccag
    15421 cgagcctcgt tgcaaccgtc ggttttcgca caaatcgcgg tatcgatctc tttggcctga
    15481 cccgtcttaa actgctcgat aaattcgtta ggcagcacaa cgttttgatc gccttcatcc
    15541 agcgtcgcac cgttggataa cgaaagattg attatctcgt tgatcacgtt gggatattcg
    15601 gcaaagacct tgcgtacatc gtccggaaca acacgagtat tattttgacc agtcgtcgaa
    15661 taacgatgaa tgagctgatc gatatttgcc ccgcgaactt catcgcccag ttcagtcagc
    15721 gcaatggtcg acttattgcc acgtactgag cccagttcaa aggtatcgat accaaaggag
    15781 atggtttcgc cccagctaaa ggagaattta ccgttttcgt cagttacgcc acggcctgaa
    15841 ttggtgtagt agtcaacgcc agcaacaccg ttccccaggc tatccaccag ttggccttcg
    15901 gaaagaatga tttcagtggg ttgatactga taaaactgtt ccgcgttagc cgacacgaag
    15961 gacgcgttca gatccggttt tgttccctct gtcgtgactg gcactatcgt tgaggtatgg
    16021 gtggacggcg ctttatcagt cgcagcattg ttttccacct cttcattgac cagcttgctg
    16081 aaattgtctg ttgccagatc aatttgctta tacagttttt caaatcgcgc gcgatcaacc
    16141 actgacgaga aagtaagaca aagctgttct gcatctgcgg ggcaactgtc gctggacgtc
    16201 accagagaga tggcgttggt tttcttattt tcagaattcg ccagctcctg cgcgtcctcc
    16261 aggctaaacg acactttgtc aaccgcacgc aggcta
//
LOCUS       DAJOTB010000110        15705 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJOTB010000110 DAJOTB010000000
VERSION     DAJOTB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.110"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            203..586
                     /gene="iraD"
                     /locus_tag="OHN28_003439"
     CDS             203..586
                     /gene="iraD"
                     /locus_tag="OHN28_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCQ2298771.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHDPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(634..3015)
                     /gene="ptsP"
                     /locus_tag="OHN28_003440"
     CDS             complement(634..3015)
                     /gene="ptsP"
                     /locus_tag="OHN28_003440"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2298772.1"
                     /translation="MVAIVIVSHSLRLAQGIEELALQMSGGDVPLAIAAGIDDPQNPI
                     GTDAIAIMSAIESVWSPDGVLVLMDMGSALLSTEMALELLSEEQRSAIYLLAAPVVEG
                     AMSAVTSAAAGLSVTEIIAEVDLALCAKQQQLMPSPPAGEVIPAISPVNHSGEWQTFC
                     WTICNPHGIHARPAASILKVSAQYSANIIVIKGDKRASTRSLNELAMLGVRCGDEIIF
                     QAEGTDASDALKAIETLAKNHFGEAHLLASSEIPLTETPVVSAPVDGAISGLSVQNGI
                     AIGPVKWFTCERPEITQRTVDSPQEELSRIESVIDIVVCELADKAAGPEGDIFAAHKM
                     MLEDPEINRQLQQSLAKGQQAEFAWLEVMQALAEQYCHAETLYLREREADIRDLTRQV
                     LNQLCGVSEQHFITTAPCILLANDLLPSQITSLNKAHILGICLHNGGTTSHTAILARA
                     MGIPAIVKAAITPQNVRDNDTVILDGETGRLWLQPDEVTRLDLLQRAEAWRQQRDRQL
                     ADAMLPAVTQGGRNISVLANIGDLQDIEAALSHGAEGVGLLRTEFLFHDSATLPDEEE
                     QFRVYCSVARVFGDKPVTIRTLDIGGDKPLPSYPLPTEDNPFLGLRGIRLCLAHPQIF
                     IPQLRALIRAGKEYPTLQIMLPMVSTLEEVRAVKTLIHAQAKLLGLTAENLPALGIMI
                     EVPAAVMIAEKLASEVDFFSIGTNDLTQYIMAADRGNSTVAELVDYRNDAVINAIAMV
                     CQAGRNNEIPVSMCGEMAGDIQQTARLLTMGIDKLSASPSRLPALKAAIRTSH"
     gene            complement(3019..3651)
                     /gene="dhaL"
                     /locus_tag="OHN28_003441"
     CDS             complement(3019..3651)
                     /gene="dhaL"
                     /locus_tag="OHN28_003441"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCQ2298773.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELT
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEEFAG"
     gene            complement(3664..4728)
                     /gene="dhaK"
                     /locus_tag="OHN28_003442"
     CDS             complement(3664..4728)
                     /gene="dhaK"
                     /locus_tag="OHN28_003442"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCQ2298774.1"
                     /translation="MKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDNAV
                     ALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFFIK
                     NYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIVGA
                     AALQGYNLGQCEQLGKDVNNATRSFGIALSACTVPAAGKPSFELADNEIEFGVGIHGE
                     PGIERRTLQDLDTLIDSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNAEY
                     IVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLKCT
                     PEFLQLWDAPVNTPALRWGC"
     gene            5054..6136
                     /locus_tag="OHN28_003443"
     CDS             5054..6136
                     /locus_tag="OHN28_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HCQ2298775.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLV
                     GDRVSQSFARENIIADFDVFCGECSQNEISRIREKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPEGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQCVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAEAATAEGE
                     TIHNMPFVVTAQDVLCAILTAHHLGL"
     gene            6203..7471
                     /locus_tag="OHN28_003444"
                     /pseudo
     CDS             6203..7471
                     /locus_tag="OHN28_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            7489..8853
                     /locus_tag="OHN28_003445"
     CDS             7489..8853
                     /locus_tag="OHN28_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2298776.1"
                     /translation="MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTM
                     IVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICREYEGRARALGFTRPEPQSISEV
                     IDTEGFKVVADQMITESGVEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVD
                     CTGDADIAARAGAPWTKRSKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKN
                     WTIQTTGKEDPMFSPYMEDIFTRAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYA
                     FGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNFGATLGTRESRLIEG
                     EIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLV
                     AGRCISAGVVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQG
                     VRLA"
     gene            9154..10695
                     /locus_tag="OHN28_003446"
     CDS             9154..10695
                     /locus_tag="OHN28_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCQ2298777.1"
                     /translation="MQYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSQYINKI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGTHADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAI
                     LICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLCGVVFILLKSFLTQLAAEEK
                     NARDE"
     gene            complement(10751..11893)
                     /locus_tag="OHN28_003447"
     CDS             complement(10751..11893)
                     /locus_tag="OHN28_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCQ2298778.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALAIGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKTVTGPLGESILAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFAQQKGASADD
                     LEQLEQGMKNYARCIYRCCGKDVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDSQSLNGKAPVGAAKVAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCNLPTALSQGENNLIRLGENLAGYFRAILS"
     gene            complement(11968..12855)
                     /locus_tag="OHN28_003448"
     CDS             complement(11968..12855)
                     /locus_tag="OHN28_003448"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2298779.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFEPAPQELIGERG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAGGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFEPGFRISLHQKDLNLALENARLLNTPLPNTAMTQQLFSACAALGGQEW
                     DHSALIRALETQANFTIRK"
     gene            complement(12873..13655)
                     /gene="hyi"
                     /locus_tag="OHN28_003449"
     CDS             complement(12873..13655)
                     /gene="hyi"
                     /locus_tag="OHN28_003449"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCQ2298780.1"
                     /translation="MAKFAANLSMLFTEYPFIERFAQASHAGFHGVEYLFPYDFSADE
                     LVSQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQKGVSRAIEYALALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVNHPDFRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            complement(13668..15407)
                     /gene="gcl"
                     /locus_tag="OHN28_003450"
     CDS             complement(13668..15407)
                     /gene="gcl"
                     /locus_tag="OHN28_003450"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCQ2298781.1"
                     /translation="MARMRAIEAAVEILKKEGISVAFGVPGAAINPLYAAMKKSGGID
                     HILARHVEGASHMAEGYTRSQNGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPVGKLHKEDFQAVDIEAIATPVTKMARTILEAGQLPGIFQKAFWEMRSGRPGPVLL
                     DLPFDVQMTEIEFDIDLYQPLTPWQPQATRAQAVRALEMLNDAEKPVIIAGGGIINAE
                     ASELLREFVELTGVPVIQTLRGWGALSDDHPLMIGRMGCQAGHRYGNASYLASDFVFG
                     IGNRWANRHTGAIETYTEGRKFIHVDIEPAQIGRIFAPDLGIVSDAESALTLFIQVAR
                     DMKSRGELKDRSRWIAECAERKRTMLRRSDFDCNPIKPQRVYHEMNKVFGPETRYIST
                     IGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAALGAVKADPSVPVVAISGDYDFQ
                     FLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAFDIDYCVQLSFENINAPEINGYG
                     VDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDEFHVPVVVEIITERVTNIAMGPD
                     INKVTEFEEILDL"
BASE COUNT         3960 a         3600 c         3845 g         4300 t
ORIGIN      
        1 aatgatacct ttttaaatta aaagtcagtg gatattcttt tctgtacagt gttttatctt
       61 tggcggatct tataaatccc ttatgaaagt gccagggacg caatgagcat aaaggaatga
      121 ttatggaata tgagcatcaa cccagtggtg tccggcagcc agggcaggat acagatgcca
      181 ctaaacgtca ggagtgcgca aaatgatgcg ccaatcagtt cagacggtat tacctgaaag
      241 tacaggaaac aatacgttat cgctgcgtga ttccgtatgc cgggatctct ttcaactttt
      301 tagttctccc cattcaccgc taccaatatt acttgtttca ggcatgcctg agtggcaagg
      361 gcataatcag agcgacaagc ttctccagag ttggtattgc cgccagttgc gttccgcgct
      421 tttatttcat gatccacgta ttgcggcatt gcaggtcaat ctgaaagaag cctattgcca
      481 cgagttggcc attagtctgg agatgatgct gtaccacgat gatgagccgc taacgtttga
      541 cctggtctgg cagaagggga gctggcatcg cacgatgccg cagtaaatga cgggaaacgc
      601 gatacgcgca agacgtatcg cgttaggtca caattagtgg gaggttctga ttgctgcttt
      661 caaggccgga agccgggatg gactggcact gagcttatca atccccatgg tgagcagtcg
      721 tgctgtttgc tggatatctc cagccatttc gccgcacata ctgaccggaa tttcattatt
      781 acgtccggcc tgacacacca tagcaatagc gttaatcacc gcgtcattac gataatcgac
      841 cagttccgcg acggtgctat ttcctctgtc tgccgccata atatattgcg tcagatcgtt
      901 ggtgccgatg gaaaagaaat cgacttcact ggctaatttt tctgcaatca tgactgcagc
      961 aggaacctca atcataattc ccagcgccgg aagattttct gcagtaagtc ccagcagttt
     1021 tgcctgagca tgtatcaatg ttttgacggc cctgacctcc tcgagcgttg aaaccatcgg
     1081 cagcataatt tgcaacgttg ggtattcttt cccggccctg ataagtgcgc gcaattgagg
     1141 aataaatatt tgtggatgcg ccagacacaa ccggatacct cgaagtccca ggaaggggtt
     1201 gtcctctgtg ggtaaagggt atgaaggtag cggtttatca ccaccaatgt ccagagtccg
     1261 gatcgtgact ggtttgtcgc caaaaactcg ggcgacagaa caatacacac ggaactgctc
     1321 ttcctcatca ggtaatgtcg cagaatcgtg gaataaaaac tcggtacgca gcaagccaac
     1381 gccctcagcg ccgtgagata gcgcagcctc aatatcttgc agatcaccaa tattcgccag
     1441 cacgctgatg ttacgcccac cttgcgtgac agcgggcagc atggcatcag ctaactgacg
     1501 atccctttgt tgccgccatg cttcggcgcg ttgcagtaaa tccaggcggg tgacctcatc
     1561 cggctgtagc cacaaacggc ctgtttcacc atcaagaatg accgtatcgt tatcgcggac
     1621 gttttgcggg gtaatcgccg ctttcacaat agcggggatt cccattgctc tggcaaggat
     1681 cgctgtatgt gaagttgttc cgccattatg aagacaaatg ccaagaatgt gtgctttatt
     1741 caggctggtt atctgtgatg gcaacagatc gttggctaac aaaatgcagg gcgcggtagt
     1801 gataaagtgt tgttcactta ccccgcaaag ctggttcagt acctgacgtg taaggtcacg
     1861 gatatctgcc tcgcgttcac gtaaatacag tgtttccgca tgacaatatt gctctgccag
     1921 tgcctgcatg acttctaacc atgcgaattc tgcctgttga ccctttgcca gactttgttg
     1981 tagttgccgg ttaatctctg gatcttcaag catcatttta tgggcggcaa aaatatcgcc
     2041 ttcagggcct gccgctttat cggcaagttc acaaacaaca atgtcgataa cagactcgat
     2101 tcgcgacagc tcttcttgcg gagaatcgac ggtgcgctgc gtaatttctg gtcgttcgca
     2161 ggtaaaccat ttaacaggac caatagcgat accattttgt actgacaagc ctgatattgc
     2221 gccatcgaca ggggcgctga ccaccggcgt ttccgttagc ggaatctctg aagaagcgag
     2281 caggtgagct tcaccaaaat gatttttcgc caacgtttca attgccttga gagcgtcact
     2341 ggcatcggtg ccttcggcct ggaagatgat ttcatcgcca cagcggacgc ccagcattgc
     2401 cagttcgttg agactgcggg tgctggcgcg tttatcgcct ttgatgacga taatgtttgc
     2461 cgaatattgt gctgatactt tcaggatgct ggctgccgga cgagcatgaa tgccgtgggg
     2521 attacatatc gtccagcaaa aagtttgcca ttcaccggaa tgattgactg gtgatattgc
     2581 tggaataacc tcgcctgcag gcggggatgg catgagctgc tgctgtttag cgcacagcgc
     2641 aagatccact tcagcgataa tctccgtcac cgaaagcccc gctgcggcag acgttaccgc
     2701 tgacatggca ccttccacta caggggcggc gagcagataa attgcacttc tttgctcttc
     2761 agaaagcagc tccagcgcca tctcggtact aagcagcgca ctgcccatat ccatgagtac
     2821 cagaacgcca tccggtgacc agaccgattc aatcgcagac ataatggcaa tagcatcagt
     2881 gccaatcgga ttttgcggat catcaatacc tgcagcaatg gccagaggaa cgtcccctcc
     2941 agacatctgt agtgctaatt cctcaatccc ctgggcgaga cgtagactat gagaaacaat
     3001 aacaatggca accataattt atcccgcgaa ttcttcctgc atagccttaa tcatcagcca
     3061 ggaggaggtc gcgcctggat cctgatggcc cacactgcga gcgccgagat aactggcacg
     3121 tccttttttc gcctgcatat tgatggtgtt atcgacggct gttgccgcat cttgcaccat
     3181 cgtattcagc agaagggaag gcgacattcc ggcttgcagg ttctttttgg cttcgtggag
     3241 gactggaacc cagacatcac acagtgtttt atccccttgt tctgcttttc ctcgtgcgat
     3301 aacgcctgca atcccttctt ccagacacgc cagccactct tccagagtta attctgttct
     3361 ggctcccact gccgcagaag cgcgaataaa taatgtgccg taaagaggac cactggcacc
     3421 gccaacgctg gaaagtagtt tcatgccagt atttttaagg atattgccaa tatgctgacg
     3481 ttcaatgtcg ggcaacgtcg ctttcacggc actgaaacca cgattcatat tgaggccgtg
     3541 gtcggcatca ccaatatctc tgtcaagtgc ggttagaaaa tcttgttgtt cagtaagatg
     3601 tgcagcacaa ctctccagcc aggcaataac atgtttgttt tgaattttca tataatgtcc
     3661 ttattaacat ccccaacgaa gcgctggcgt attgaccggg gcatcccaaa gttgcaaaaa
     3721 ctctggggta catttcagca ggctaatgga aaatccggcc atatctaatg atgtacaata
     3781 attaccgacc aactggcggc taattttaat tccctggcgc tgtgcggcgc acataaatac
     3841 cctggctaca ccatataact ctgattcagg cgtgctgcca agtccgttaa ttaacacaat
     3901 atattcggca ttttgatcga atgactcatt cattgatgat gcgtcaatcc atccccccgc
     3961 atggcgatcc caatggcgta atgtgcgtcg ccagggggta ttatccagta attgcgctat
     4021 cacgctgtcg attaaagtgt ccagatcttg caaagtgcgg cgttcaatgc cgggttcacc
     4081 gtggattccg acaccaaatt cgatttcatt atcagccagt tcaaatgagg gttttcccgc
     4141 ggcgggaacc gtacaggctg acagtgcgat gccaaaagaa cgtgtggcat tgttaacatc
     4201 tttgccaagt tgctcgcact gccccaggtt atatccctga agtgcagccg caccgacaat
     4261 cttttcaaca aaaactgtac ccgcaacacc gcgacgtccg gctgtataca ggctatcttt
     4321 taccgctaca tcatcatcaa taatgacggt cccaacggcg atgccttctt cgtgtaacat
     4381 ctcgactgcc gtttcgaaat tcagaatatc gccagtgtaa tttttgatga aaaacagaac
     4441 gccagaacca ttatcaaccg ctttggcaca ttcgatcatt tgatctggcg tgggcgatgt
     4501 gaagatttca ccaggacatg caccggttaa cattcccata ccgacaaaac ctgcatgcag
     4561 tggttcatgg cctgagccgc cgcctgaaat cagcgccact gcattatcgg tttgtttgca
     4621 ccacacatat cggggcgcgt aatttgcctg caggtgtggc caggtggcga tgagtccttc
     4681 catttgttct gtgacaacag aatcaatctg gttaattaat ttcttcatag tcattcctgt
     4741 tctgataaat tacgtaaatc gcgctaaata tgctttcgag attaattctc cagccgcaat
     4801 aaaacaaatc gcacgataat aagattcata atgaaacagt taattcattg tgttgttcca
     4861 aaatgaaatg ggaccagcgt ggatattgat atattgtgat taaggcatga aaagtgtttt
     4921 ttattatcat ctgcagatga tgttttttac agcgcgatca taaaacataa tttttcgcga
     4981 atgaaatgtt tgccgccact ggatgcaacg agtaggttaa cagtgaagca aaacaagtta
     5041 cgcgaggaat gatatggata agattattat aagtccggct aaatatatcc agggaaatgg
     5101 ttctctggat aatattgcta cgtatgcggc gagtttggga acagaaccat taattattgc
     5161 agatgaattt gtgaccggtc tggttggtga cagagtaagc cagagctttg caagagaaaa
     5221 cattattgcg gattttgatg ttttttgcgg tgaatgctcg caaaatgaaa tttctcgaat
     5281 tcgtgagaaa tttaatcagc gtaaatataa tgtcgttatt ggtattggcg gcggtaaaac
     5341 gttggatacg gcaaaagccg tagcgtatta ccaaaaaatt ccggtggtgg tggtgccgac
     5401 aattgcttcc acagatgcgc caaccagttc tctggcagta atttacaccc cggaaggcca
     5461 gtttagcgaa tatctgtttt tcccgaaaaa cccggatatg gtgattatgg atactggcgt
     5521 catttcagcc gcgccagtgc gtctgctggt ggccggtatg ggagatgcat tgtcaacctg
     5581 gtttgaggcg agagccaatc aggcgtcagg aaaagccaca atggcaggtg gggcgtcgac
     5641 actggcggca ctggctattg cccgactctg ttataccacg ctactggaag atggctataa
     5701 ggcaaaagtc gcggttgaac aatgtgttag taccaaagcg gtagagaata ttatcgaagc
     5761 taatacctat cttagcggca ttggatttga aagtagcggc ctggcggcag ctcatgccat
     5821 tcataacggc atgacgcaac tggaggaatg ccatcattgc taccacggtg aaaaagtggc
     5881 atttggcgtc ctggtacagc tcgtactgga aaacgcacca caggaggaaa ttgaaaccgt
     5941 actcaatttc tgccatagcg ttggcttgcc gacaaatctt catatgttag gcgtgaagga
     6001 aattaataaa gataaattac gggcagtggc tgaagccgcg actgctgaag gggaaactat
     6061 ccataatatg ccattcgtag taacagcgca ggacgttctt tgcgcaattt taactgcaca
     6121 tcatttggga ctttaaaaaa taatacggcc tgtgcaaagc aggccgttgg tgtcatttta
     6181 aatacttcat tcgggaataa atatgaccag taactggaaa cgttggatca cgctggcgtt
     6241 atgtggtatt gccggaagtg caatatataa attgccttat ttacgggaaa cctattacga
     6301 cgcgatgcag caggctacag gagcgactaa tgctgaactt ggttttctaa tgacagccta
     6361 tggattggtg aactttttat tatatctacc tggcggttag gctgccgata gattttctgc
     6421 acgtaaatta atgacctttt cattaatttc caccgggata agcggttttt attatgcgac
     6481 cttcccctcg tataccatga tctgtttgtt acatgcactt tgggctgtta caaccgtatt
     6541 tacgttttgg gccgtgtgtg tgcgaattat tcgtaccctg ggaaccagcg aagaacaagg
     6601 tcgcttatac ggttactggt ttctgggtaa gggattaacc tccattgtac tgggatttct
     6661 ttccgtgccg gtatttgcca aatttggcga aggtgttgat ggtttacggg cgacaattat
     6721 tttttattcc gtagtgacta ttctggccgg tgttttagcc tggtttgttt gtcaggatga
     6781 aacacacagc gaagataaag cgaatttccg tctggcagat atggcattcg tgctgaaaat
     6841 gccgacggtt tggctggcag gtgttgtgac cttttgtatg tggtcaattt atatcggctt
     6901 tggcatggtc acgccgtatc tcacgcagat cctgcatatg ggcgaatcgg aagtggcagt
     6961 tgccagtatt cttcgcgcct atgtgttatt tgcgatgggg ggattaattg gcggacaact
     7021 ggccgaccgc tgtgcgtcac gcacgcgttt tatgatttac gcctttatcg gcatgattgt
     7081 ctttactacc gtttatttct tcctgcccgg tgagtcgcgc tacgtcacga ttgcgctggc
     7141 gaatatggtt gcgttgggcg tgtttattta ctctgcgaat gccgtgttct tctcaattat
     7201 tgatgaggtt cggatcccgg caaaagtcac agggactgct gcgggtctga tctctttact
     7261 gacctatttc ccggaaatct actgctacac gatggtgggc aatatggtcg accgcaaacc
     7321 gggtattgcc ggttatcagg atgttttcct gttcatgttg gtttgcgcat tcatcggcct
     7381 tatcgccgca ctggtcctcc agcgtgtgaa tcgtaaaagt aaaaatcaag tcaatgaaat
     7441 cactgaaaac aatgcggatt gcgcatcctg aatctgacgg gggaaccaat ggtggacatg
     7501 attaatgaaa gtgcacggca aacgccagtc attgcacaaa cggacgttct ggttatcggg
     7561 ggcggtccgg caggattatc cgctgccatt gcggcagggc ggttaggtgc cagaaccatg
     7621 attgttgagc gttacggctc actaggcggc gtattgacgc aggttggtgt ggaaagtttt
     7681 gcctggtatc gccatccggg gacggaagat tgtgaaggga tctgtcgtga gtatgaaggc
     7741 cgcgcacgag cgctgggttt cacacgacca gaacctcagt caattagcga agttatagat
     7801 actgaaggat ttaaagttgt cgccgatcag atgattacgg aatctggcgt tgagccgtta
     7861 tatcactcct gggttgtgga cgtgatcaag gacggggata cgttatgcgg tgttatcgtc
     7921 gagaataaat caggtcgagg ggcaattctg gcgaaaagaa tcgtcgattg cacgggggat
     7981 gctgatattg ccgctcgtgc aggcgcgccc tggacgaaac ggagcaagga ccaactgatg
     8041 ggcgtcaccg tgatgttcag ttgcgcaggt gttgatgtgg cacgctttaa ccgttttgtt
     8101 gcggaagaac ttaagccgac ctacgcggat tggggcaaaa actggacgat tcaaaccacg
     8161 ggtaaagaag acccgatgtt tagcccgtat atggaggata tttttacccg cgcgcaacag
     8221 gatggtgtga ttccaggtga cgcccaggcg attgccggaa cctggtcgac cttttctgaa
     8281 agcggtgagg ctttccagat gaatatggtg tacgcctttg gttttgactg taccgatgtc
     8341 ttcgatttaa ccaaagctga gattgccgga aggcagcaag cattatgggc aattgacgca
     8401 ctacgccact atgttccggg ctttgaaaat gtacggttac gcaattttgg tgccacgctg
     8461 gggacgcgtg aatcacggct tattgagggg gaaatacgta ttgctgatga ttacgtcctt
     8521 aaccaggggc gttgttcgga cagtgtaggg attttcccgg aatttattga tggttccggt
     8581 tatctcattt tgccaacgac cgggcgtttc tttcagatcc cttatggttg tctggtgccg
     8641 caaaaagtgg agaacctttt ggtcgccggt cgctgtattt ccgcaggcgt agttgcacat
     8701 acttctatgc gtaacatgat gtgttgtgcc gttaccggtg aggccgcagg tactgccgcc
     8761 gtggtttcgc tacagcaaaa ttgcaccgtg cgtcaggttg ctatccctga tttgcaaaac
     8821 acgctgcaac agcagggcgt acgtctggca taacaacaag gcgcagagag ctgaaagcct
     8881 gtctgcgcct tgcaggagaa agaccatgag ggacgatgac cttgagcact taagtgccaa
     8941 ttactggata accacagttc tctgcggcct ctttttgggg atattcctgt tcatgctgca
     9001 acagcatcag cgtggtttag cgctgtattg tattgcggca ctttcactta tttgcgcctt
     9061 tttttcttta cgcctttccc gacaattgca acacatgaaa aaggaacggc gaaatattcg
     9121 tcctcattga atgaaaacat tcaggagact gaaatgcaat atttaaaaaa acgcttttct
     9181 ttgattgaac tgaatgtgtt tatcccagca atattattta ttgcggtaat cattctgtgc
     9241 ttgacgatat atccgcaaga taccagtcag tatataaata aaatacatca ttttttgacc
     9301 tgggaaatgg gtggaatttt tctggtcatg acttttctgg ttgtactttg ttgtctgtgg
     9361 ctggccttct cacgttatgg cgatatcttg ttaggtcagt cgggagaaaa acctgacttc
     9421 agcttgttaa cctggcttgg acttattttt acttctggaa cggggggcag cttgttatat
     9481 ctggcctctg tagagtggat ttggatcatt cagcaaccgc cttttggcgc aacagcagga
     9541 agcgctcagg ctgctcgttg ggcctctgct tatggcatgt tccattgggg gccgtccgca
     9601 tgggcatggt atttgatttg cgccgttcct attggttggt ttatgcatgt taagaaaacg
     9661 aactcattaa aggtcagtga tttatgtcgt ggatgcctgg ggacgcatgc tgatggtttt
     9721 tgcggacatt gtgtaaactt tttctatatg tttggtttgc tcggcggcgc ggtaacgtcg
     9781 ctggcgctgg gaacgccgat gatttctgcc gtattttgcc atgtgttcca tctggatcct
     9841 gccgggcagt ttatcaatgt catggttatt tttatctgga cgctagtacc attattcatt
     9901 ctcttttttg gacttaaaaa aggtgtggca tgggccagta actggaatat tcgtgctgac
     9961 attttgatgc tactggcaat actgatttgt ggaccaacgg catttatact taatcaatca
    10021 attgatggtc tcggcctgat gctgcaaaat tttgtagcga tgagtttaag taccgatgct
    10081 attggtcgta gcggatttcc acagatgtgg accgtatttt atttttcatg gtgggtcgtg
    10141 tatgccatcc cattcggttt atttatcgcc cgtatttcaa aaggaaggac gatccggcaa
    10201 ttgattgtat gtggaactct ggcaggttca ttgggatgca tggtttttta catggtactg
    10261 gctaatttcg gcttatcgtt gcagacaact catgtcatcg attttgttcc catacttaac
    10321 gaacaagggc gaggcgttgt cgtttctcgt ttactggagc agctacccgc aagtcaggtg
    10381 tttttggttg cttttggggc tatagcatta atttcatata ttaccggaca ctgtactgtg
    10441 ggttatgccc tcggttttgc gacgcaaaaa cgggcagata gtgagagtga accggcattc
    10501 tggaacgtgg cattttggtt gattatgact ggaatcgtcg ccatcacact ctatcttctt
    10561 gatgcgcaga gtttgcagcc gctacaaacg gtctctatcc tggccggact accgctttgc
    10621 ggcgtagtgt ttattttatt gaagagtttt ttgacacagc ttgcggctga agagaaaaac
    10681 gcgagagatg aataatgatg ttcaggaatg cccggtaatt tgcattatcg ggcattttac
    10741 cgaacgggaa ttaactcagg atcgcacgaa aatatcccgc cagattttct ccaagacgaa
    10801 ttaaattatt ttctccctga ctcagcgcgg tcggtaaatt gcaagggccg ttgacgacag
    10861 aaaaacaggc ttctattcca cactggcgta actcattcag atcaagttga tcatcgatat
    10921 acccggcaat tgcgataacc ggaacgccca tcatctgggc aactttggct gcgcctacgg
    10981 gcgctttgcc attcaggctc tgactatcta atttcccttc accgacaatg gccaatgctg
    11041 cgtattttaa atgttgggta tattgaatcg cttccagcac gagcgaaata cccggttgta
    11101 agcgagcatc gagaaaagcc atcaaggccg cgccaacgcc gccagccgcc ccggaaccgg
    11161 gtatcgtatc gacgtcttta ccacaacaac ggtagatgca acgggcatag tttttcattc
    11221 cctgttccag ttgctcaagg tcgtcagcac tggccccttt ttgttgagca aaaacccggg
    11281 tcgcgccgtt atcgccggtt agtgggttgg tgacatcaca ggctgcaata aatttcacat
    11341 ttttcaatcg tggatcgagg tgctgtaagt caatgtggtg aattgccgcc agccccatcc
    11401 cattaacgga gactgatagc ccttcggcgt ctgtaaacga tgcgccaagt gccgacatca
    11461 acccaatacc gccatcattt gtagcactgc cacccaggca tagaataacg gtatcaatat
    11521 tgtgttccag ggcggcgaga ataagttcgc cagtgccaaa cgacgtcgta taaaggggag
    11581 aacgctggac aggagaaacc agttccagcc ctgaagcttg tgccagttct atcactgcgg
    11641 tggtttggtt gcctaaaata ccgtattgcg caagtattga ttcgccaagc ggtccggtga
    11701 ctgttttagt gtaaaacgta ccgtcagtcg catgaatgag tgtttcgagc gtgccttcgc
    11761 cgccgtcagc cacgggcaaa cacaccagtt ggctgccagg caggaccttg cgccagccga
    11821 ttgccagcgc ttcggcgact ttccatgcag gaaggcattc tttaaaggag tcgggggaga
    11881 taactatctt catagcaggt ctctcaaaat aggggaatgg ggcagggcaa cgagtgaacg
    11941 aagggaaccg gtggttccct taattcatta tttacggata gtgaaattgg cttgtgtttc
    12001 aagcgcccga attaacgcag aatgatccca ctcttgacca ccgagcgcgg cgcaggcgct
    12061 aaataattgt tgcgtcatag ccgtgttagg aagaggggta ttcaacaggc gagcattctc
    12121 cagtgccaga ttcagatctt tttgatgcag actaatacga aaaccgggtt cgaatgttcc
    12181 tttaatcatc cgttcgccat gaacttccag cacgcgagag gaggcaaacc ctcccatcag
    12241 cgcttcacgt acgcgggcgg gatcggcacc tgatttactg gcaaagagca gggcttcagc
    12301 cactgcttca atattcagtg caacaataat ttggttagcg actttgcacg tttgaccatc
    12361 accgacgtta ccgaccagag tgatattttt acccatcaat tccagaatcg gtttgatctg
    12421 taaataaacc tcttcacatc cgccaaccat aatggaaagc gtcccggctt tcgcgccaac
    12481 ttcaccgcca gagacgggcg catcgatata ttccgcgcct cccgcgataa tacgttgtgc
    12541 gaatgctttg gtggcaaccg gggaaattga actcatatca atcacaattt tcccgtggct
    12601 taaaccgtgt attacgccat agttgggatg gaaaagaaca tcctcaacat gtggggtatc
    12661 agggagcatg gtgatgatga cctcacactc ctgcgcgact tcacgcggtg tagagcaaac
    12721 aatgccacgt tcaccaataa gctcttgcgg tgcaggttca aaatgcgctg aaaaataaag
    12781 agtatgtccg gcttgttgta aattataagc catcggctta cccattatgc cggtgccgat
    12841 aaatccaata ttcatgataa atacctcagg gattaaaatt gagtctcgtt ttttagccag
    12901 cgtagacttt cagtcgttgt cgtagatgga atatattcgc aaccaatcca tccctgataa
    12961 ccaatctggt ctatataatt taacaaccac gggtaattga tttctcccgt ccctggttca
    13021 tgacgcccag ggttatcggc taattgaata tgtccaatgt tattcaggtt gtttttgatt
    13081 gttgtagcaa tattcccttc catgatttgc atatgataaa tatcgtattg atagcgaaaa
    13141 tcaggatgat ttacatcatg aataatattg agagcttgtc tggtgttgtt aacaaaaaaa
    13201 ccgggaatat cttttgtatt aatggcttcc agaactaact ttatgccatg agaagccaat
    13261 ttatctactg catagcgcaa attttcgacc agtgtttcat gacattgttc atgcgaataa
    13321 ccacctggat gttttcctgc aaggcaatta acttgtgaac aattaagggc caatgcatac
    13381 tctattgccc tggaaacgcc cttctgaaat tcttttgccc gaccaggatg acatgcaata
    13441 ccccgttcac cctcttgcca gttacccgca ggaagattaa acagaacctg cgtcagatta
    13501 tgttgatgta gttgcgaaac caactcatct gcggaaaagt cataggggaa aagatactca
    13561 acgccgtgga atcctgcgtg acttgcctgt gcaaaacgtt caataaatgg atactcggta
    13621 aagagcatag ataaatttgc tgcaaacttt gccataatcc tgtttcctta taaatcgaga
    13681 atttcttcaa attctgtgac tttattaatg tcaggtccca tagcgatatt agtcacgcgt
    13741 tcagtaataa tttccacaac aacaggtacg tgaaattcgt cacgcaaacg ctgggcttct
    13801 tgcagtgcag gcgcaatatc ctgtgatgcg aatacgcgaa tcgctttaca tcctaatcct
    13861 tcaaccacag ctttatgatc aacgccataa ccattaattt ctggagcatt aatattttca
    13921 aatgacagtt gaacgcaata atcaatatca aatgcacgtt gtgattgacg aatcaaacca
    13981 agataagcgt tgttaagtaa aatatggata tacggaagat taaattgtgc gccgacggct
    14041 aattcttcaa tcaagaattg aaaatcgtaa tcaccggata ttgccacaac gggaacagat
    14101 ggatcagctt ttaccgcgcc cagtgctgcg ggcattgtcc agcctagcgg cccagcctga
    14161 caggcattaa tccagtggcg tgggcggtaa acatgtaaaa attggtttgc ggcgatttgc
    14221 gccagaccaa ttgtcgaaat atagcgtgtc tccggtccga aaaccttatt catttcgtga
    14281 tacacgcgct gcggtttaat tggattgcag tcaaagtctg aacgacgaag cattgtgcgt
    14341 ttacgctcgg cacattcggc aatccagcga ctgcggtctt tcagttctcc acgtgacttc
    14401 atatcacggg cgacctgaat aaatagtgtt aaggcgcttt cagcatcaga aacaatgccc
    14461 agatccggcg caaatatccg gccaatttgt gcaggttcga tatcaacatg aatgaatttg
    14521 cggccttcgg tgtaggtctc aatggctccg gtatggcggt ttgcccaacg gttaccgata
    14581 ccaaaaacaa aatctgaggc cagataactg gcgttaccat agcgatgacc cgcctgacat
    14641 cccatgcgac caatcattag ggggtgatcg tctgataaag cgccccaacc gcgtaaggtt
    14701 tgaatgacgg gaacgccagt taactcgaca aactcacgca acaattcact cgcttcggcg
    14761 ttaataatgc caccgccagc gatgatgacg ggcttttcgg cgtcatttag catttctaat
    14821 gcgcgtactg cctgggcccg tgttgcctga ggttgccagg gggttagtgg ctgatagaga
    14881 tcgatatcaa actcaatttc ggtcatctga acatcaaaag ggagatctaa aagaaccggg
    14941 ccagggcggc ctgaacgcat ttcccagaag gctttctgga atatccccgg caactgaccc
    15001 gcttcgagaa ttgtgcgagc cattttggtt actggcgtgg cgatagcctc aatatcaacg
    15061 gcctggaaat cttctttatg aagttttcct acgggagctt gaccagtaat gcagagaata
    15121 ggaatggaat ccgcagaggc agaatacagc ccggtaatca tatcagtgcc cgcagggcct
    15181 gatgtgccaa tacacacgcc aatattgcca ttctgactgc gggtgtaacc ttcagccata
    15241 tgcgaggcgc cctcaacatg acgggccaga atatgatcaa taccgcctga ttttttcatc
    15301 gcggcataga gtgggtttat tgctgcgccg ggtacaccaa aagcaacgct aatgccttct
    15361 tttttcagta tttctactgc agcttcgatt gcacgcatcc tcgccataca ttatttctcc
    15421 tgatatttac gctgagttga atagtcagtc aattttggaa tttaacgatg aaatatcttt
    15481 gaggaaataa aagggcagaa caaatttcgc tgcactgttt cacagtgaaa caactcgtat
    15541 gcctgtgttg tttcagtttg aataattata aatatcaaac gcaaagattt cagatgttgt
    15601 tggcagtttt aaaatttatc taatgtatca ttcgtttttt attgctttaa cggttattat
    15661 ttctaatgta tgtgtgatta cattgttgat aaggtctgtt tttgt
//
LOCUS       DAJOTB010000111        15701 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJOTB010000111 DAJOTB010000000
VERSION     DAJOTB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.111"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            89..1063
                     /gene="hemB"
                     /locus_tag="OHN28_003451"
     CDS             89..1063
                     /gene="hemB"
                     /locus_tag="OHN28_003451"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCQ2298782.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1103..1954)
                     /gene="tauD"
                     /locus_tag="OHN28_003452"
     CDS             complement(1103..1954)
                     /gene="tauD"
                     /locus_tag="OHN28_003452"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCQ2298783.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1951..2778)
                     /gene="tauC"
                     /locus_tag="OHN28_003453"
     CDS             complement(1951..2778)
                     /gene="tauC"
                     /locus_tag="OHN28_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCQ2298784.1"
                     /translation="MSVLINEILHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2775..3542)
                     /gene="tauB"
                     /locus_tag="OHN28_003454"
     CDS             complement(2775..3542)
                     /gene="tauB"
                     /locus_tag="OHN28_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCQ2298785.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3555..4517)
                     /gene="tauA"
                     /locus_tag="OHN28_003455"
     CDS             complement(3555..4517)
                     /gene="tauA"
                     /locus_tag="OHN28_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2298786.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4823..5098
                     /gene="frmR"
                     /locus_tag="OHN28_003456"
     CDS             4823..5098
                     /gene="frmR"
                     /locus_tag="OHN28_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCQ2298787.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            5133..6242
                     /gene="frmA"
                     /locus_tag="OHN28_003457"
     CDS             5133..6242
                     /gene="frmA"
                     /locus_tag="OHN28_003457"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCQ2298788.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            6335..7168
                     /gene="fghA"
                     /locus_tag="OHN28_003458"
     CDS             6335..7168
                     /gene="fghA"
                     /locus_tag="OHN28_003458"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2298789.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(7293..7832)
                     /locus_tag="OHN28_003459"
     CDS             complement(7293..7832)
                     /locus_tag="OHN28_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCQ2298790.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7934..9145)
                     /gene="mhpT"
                     /locus_tag="OHN28_003460"
     CDS             complement(7934..9145)
                     /gene="mhpT"
                     /locus_tag="OHN28_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCQ2298791.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(9321..10334)
                     /gene="dmpG"
                     /locus_tag="OHN28_003461"
     CDS             complement(9321..10334)
                     /gene="dmpG"
                     /locus_tag="OHN28_003461"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCQ2298792.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGGDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(10331..11281)
                     /gene="mhpF"
                     /locus_tag="OHN28_003462"
     CDS             complement(10331..11281)
                     /gene="mhpF"
                     /locus_tag="OHN28_003462"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCQ2298793.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(11278..12087)
                     /gene="mhpD"
                     /locus_tag="OHN28_003463"
     CDS             complement(11278..12087)
                     /gene="mhpD"
                     /locus_tag="OHN28_003463"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCQ2298794.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(12097..12963)
                     /gene="mhpC"
                     /locus_tag="OHN28_003464"
     CDS             complement(12097..12963)
                     /gene="mhpC"
                     /locus_tag="OHN28_003464"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCQ2298795.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12981..13925)
                     /gene="mhpB"
                     /locus_tag="OHN28_003465"
     CDS             complement(12981..13925)
                     /gene="mhpB"
                     /locus_tag="OHN28_003465"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCQ2298796.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13927..15591)
                     /locus_tag="OHN28_003466"
     CDS             complement(13927..15591)
                     /locus_tag="OHN28_003466"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCQ2298797.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
BASE COUNT         3651 a         4564 c         4017 g         3469 t
ORIGIN      
        1 ttgtcaacat cgcgacaact ttcgtaaaac acacccagcc tgcttcaggt atactatgcc
       61 cctcgattcc acaaacatca ggcagaccat gacagactta atccaacgcc ctcgtcgcct
      121 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      181 ggtgttgccg atctttgttg aagaagaaat tgacgactat aaagccgttg aagccatgcc
      241 aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
      301 cggtattcgt tccgtgatga ctttcggcat ctctcaccat accgatgaaa ccggcagcga
      361 tgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agaccgtgcc
      421 agaaatgatc gttatgtcag acacctgctt ctgtgaatac acatctcacg gtcactgcgg
      481 tgttctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag gcaagcaagc
      541 cgtggttgca gctgctgcag gcgcagactt catcgcccct tctgccgcga tggatggtca
      601 ggtacaggcg attcgccagg cgctggacgc tgcgggcttt aaagatacgg cgattatgtc
      661 gtattcgacc aagttcgcct cctcctttta tggcccgttc cgtgaagctg ccggaagcgc
      721 attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgtg aggcgattcg
      781 tgagtcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgccggagc
      841 atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
      901 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
      961 gaaagtcgtg ctcgaaagct taggttcgat taagcgtgcg ggtgcggatc tgattttcag
     1021 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taatgatcat tatgccgggt
     1081 tatccaaccc ggcatttccc ccttaccccg cccgataaaa cggaatatcc ccaagaatcg
     1141 tcgcccgatg cataatccgc cgctgtggca ggtaatccgc attggcatag tgctgagtca
     1201 ctcggttatc ccaaatcgca atatcattcg gttgccagcg ccagcgcacc tgaaactccg
     1261 gtttagtgat atgggcaaac aaaaaaccta acaaggcttc gctctctttc tcgctgacat
     1321 caacaattcg tgtggtaaaa ccttcgttca caaacagtgc ctgtttaccg gtcaccggat
     1381 gcgttcgcac caccggatgt agcaacggtg gatttttcgc gaccgcctca cgccagcgtt
     1441 gatgctcagc ctcggttttg cggtacttgt attccgggaa cgatttacgg aaatcatgct
     1501 ccgcacgcaa cccgctcaac agctggcgga agggaacaga gagcgcctca tatgcggcaa
     1561 taccactggc ccagagcgta tcgccgccgg tcgagggtaa ctctttcgcc gccagaatcg
     1621 cccctgcggg tggcgtttga ataaatgtca catcggtgtg ccagttgtcg ttatccggcg
     1681 gattatcatt atgggtatcc aggacgatga tctcgtcaac cccttcagca tgagggtaaa
     1741 cagggtgaat atgcagctcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgtga
     1801 tagcttgatc gcgcagaaac accacctgat ggcgcagcac cgcatggtaa agctgttcaa
     1861 actgattatc gcttaacggg cgcgttaagt cggcccccgt aatttgtgcg ccaatatacg
     1921 gccccagcgg ggtaatgctc agacgttcac tcattgtact tctccatgcc agggcgtcag
     1981 gcggcgctgt aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc
     2041 gatccccgcc agcaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc
     2101 taaaccgcgc gttgcggcaa tcagctccgc cgccaccagc gtagaccagc ccacacccag
     2161 accaatgcgt aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag
     2221 cacctgcgca cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct
     2281 tttcaccccc gccagcgccg acatcgccac cggcgcaaaa atcgctaaat agatcagcaa
     2341 gatcttcgag gtttcgccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg
     2401 cggcaccgga cgataaagct cgattatcgg atccagaatg ccgcgcaccg tcgggcttag
     2461 ccccatggcg atcccgaccg gaataccgat caacaccgcc gcgagtagcg ccagcaaaat
     2521 gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggccc
     2581 ggcaatggtg agcagttttg ccagtacctg ttgcggtggc ggcaaaaaca gcgggctaat
     2641 cagttgcagc gccgcgactg cccaccatac agtgagcaaa accgctaacg taccaatgct
     2701 taaggtcacc tgacgcgaga gcggccagcg ccatttcagc cgatgcgaat gcagtatttc
     2761 attaatgagc acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat
     2821 tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca
     2881 aagcggcgag caaagttgag cggcagccgc tccagcacac ggcctgggcc gggtgaaagc
     2941 aaaaccagtt cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc
     3001 tgctttcccg tctcctgcca aagtttcagc agcagggttt gcatctggtc gcgggtgaag
     3061 gcgtccagcg caccaaacgg ttcgtcgagt aataacagct ggggatttgc cgccaacgcg
     3121 cgggcaatcc ccacccgctg acgttgacca ccggaaagct gccagatgta gcgtttttct
     3181 gcgccttcca gcccgacttt tttcaccatc tggtgtgcga tttccagtcg ctgcattttc
     3241 tctacacctg ccagttgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtaac
     3301 aacccttcat tctgaaaaac tacgccacgc tctgctcccg gtccctgaat acccttaccc
     3361 gccagttgaa tgctgccatg ctgataaggc acaaaaccag caatcaaatt cagcaacgta
     3421 gttttgccgc agccagacgg ccccagaact accagaagct cgccgctttc cagcgtcagg
     3481 ttgatatctt ccagtgccgg tttgccgcca taatcggcgt aaagatgaga aatttgcagc
     3541 atctgcgcct ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct
     3601 acagccggga ctttgccctg ctctttcaaa aactgcgcgg tgtcgatgat cgctttgttc
     3661 accggtccgg tcagttctgc cgtttgttgc tgcggcgtca gataggtatt ccccttcacc
     3721 agccccggaa cgtcaccttc cggcacgccg cttaaccgcg ccagtttgct gatgttttcc
     3781 ggctgtttca gccacgcgtc tgggttagca atgtacggtt gctgagcatc gatggcgctt
     3841 ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa aatctttccg cacgacccag
     3901 acgtccagtg tcggcgcgcc ccactgcccg acctgttcag aatcggtcag caccttgcca
     3961 tctttttcca gggcgttaac cgccggtgcc catacataag caccatcaat atctccccgc
     4021 tgccatgcgg cgataattgc gggcggctgc aggttcacaa tctcaacttg cccaggttta
     4081 ataccccagt gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg
     4141 gcgatgcgtt taccaatcaa gtcttccggt ttactgatag ttttctttac caccagcgct
     4201 tcggagttac ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggtt
     4261 gcaaccgcta acgggctgga accgaggttg ccgatttgca catcgcccga agccagcgcc
     4321 cgcacgatgc tggctccact gtcaaactta cgccagtcca cggttgctcc gctttcttta
     4381 gcaaaggtgt tgtcggcctg agccactttc gccggttcgg cggaggtttg atacgccacg
     4441 gtgacgttca ccgcttgcgc ctgaaaagcg atgaatgcca gtgcggcaag aaatgtgtta
     4501 cgcgatgaaa ttgccatgat tgtctgctcc cccgtcttgt tatgggagca gtatttcgtt
     4561 gcgggtaagt ttgataaagg aattaaaacg tcttgttaat aacaaagcgt ttttagaaaa
     4621 aaatcggcaa tggttagcga gtttgcgcat tttgctactt ttaacaaaca tcgcctgatg
     4681 tgacgctcac gcctcttatc atgcctacaa atactggggc cggataaatt ttattctcca
     4741 gtgttatata ctataggggg gtatgcattg acatatagca taccccccta tagtatattg
     4801 cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa aggtccttac
     4861 tcgagttcgt cgtattcggg ggcagattga tgctctggaa cggtcgctgg agggtgatgc
     4921 cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta acggactgat
     4981 ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact gctacagccg
     5041 tgaagtcagc caatccgttg acgacactat tgaactggtt cgtgcctatc ttaaatagct
     5101 gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc gttgcatttg
     5161 ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa aaaggtgaag
     5221 tgctgattaa agtcacccat accggtgttt gccataccga cgcatttacc ctctccggcg
     5281 atgacccgga aggtgtcttc ccagtggttc tcggtcacga aggggccggg gttgtagttg
     5341 aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatccca ctttacaccg
     5401 cagagtgcgg cgagtgtgag ttttgtcgtt ctggcaaaac caacctgtgt gtcgcggttc
     5461 gcgaaacgca gggcaaaggc ttgatgccag acggcaccac ccgtttttct tacaacgggc
     5521 agccgcttta tcactacatg ggatgctcaa cattcagtga atacaccgtg gtagcggaag
     5581 tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgcctg ctgggctgtg
     5641 gcgtgaccac cggtattggc gcggtacaca acacagctaa agtccagcca ggtgattctg
     5701 ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg cgtcaggcaa
     5761 aagcgggacg gattatcgct atcgatacca acccgaagaa attcgaactg gcgcgtcgct
     5821 tcggtgccac cgactgcatt aacccgaatg actacgacaa accgattaaa gatgtcctgc
     5881 tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac gtcaacgtga
     5941 tgcgtgcagc gctggaaagc gcgcaccgcg gctggggtca gtcggtgatc atcggggtag
     6001 caggctccgg tcaggaaatc tccacccgtc cattccagtt ggtcaccggt cgcgtatgga
     6061 aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg gttgaagatg
     6121 cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc ctggatgaaa
     6181 ttaatgacgc cttcgacctg atgcatgaag gtaaatccat tcgaaccgta attcgttact
     6241 gatttcccgc tggtttaccc cgtccagttc agatggggtt cttaatactc tccccgggca
     6301 gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa aacatgccag
     6361 ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat gtgaaatgaa
     6421 tgtcggcgtc tatctccccc caaaagccga gaatgaaaaa ttgccggtgt tgtactggct
     6481 ttcaggcctg acctgcaacg agcaaaattt cattactaaa tcggggatgc agcgttacgc
     6541 ggctgagcac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca gtcatgtcgc
     6601 agatgctgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg cgacgcaagc
     6661 gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc cgaatttagt
     6721 gatgcatcat tttccggcaa cggccagaaa gtcaatcgca ggtcattcta tgggcgggct
     6781 gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct cggcgttttc
     6841 gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg catatcttgg
     6901 tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac aaggtcaacg
     6961 cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcgg aacaactgcg
     7021 gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa tccgttatca
     7081 cgagggttat gatcacagct attattttgt ctccagtttt attggcgagc atattgccta
     7141 ccacgccaat aaactgaata tgcgttgagc atccgcgcac gcatgccgga tgcggcgtaa
     7201 accttatccg gcccacattt cgctcgtaaa ccgtaggcct gataagacgc gaaaagcgtc
     7261 gcatcaggca tctgcacacg atttatcaat gtttaccaca tcaaatcatc aggcactttg
     7321 aagtcggcat acggatcgtc ttcatcctgc tcttcggcgc tgagcgcgct gtgcaacaca
     7381 atactgctgg catcacgctg agcaatttta tcggcaacgc tggcgggaat aatggcgtat
     7441 tcaccttcgc tattgttatc gaccaacaag cgggcaatcg ccagacgacc attaatcaac
     7501 tgcgtttgcg tgagcttatc gacaaaaatc ttcttaatca gattgccgtc agtgaagtta
     7561 aaaccaatat cgccattggc aatggtaatt cggttcattt caatgagctg cttcacctgg
     7621 gctttatatt ctttcgccaa cgccgcttgt ttttgttgct cgctaagctg tttatcacgc
     7681 tcaagctgtg cctttttatt ttcttctacc gccgcccgtg cttcacgcgc ctgaacacgc
     7741 gattttttcg ccgttctctc gaccttcgcc gcttttttgc tggtgactag tcccgctttg
     7801 agcaactgct cttgtaaggt aagttttgcc atctttgttt ccaaacccgc tgaataattt
     7861 ttagcgagta tacctgtaaa cgccgtaggt gtattggggg caagtccctt tcgcccctgc
     7921 ttaataaggc gcgtcaggca tcggcacacg gctgcattct tgatctccga ctcatcaaaa
     7981 taaacaccgc caacccagca acaagaatac ccggtgcaga agcggccatt acgccgaccg
     8041 tgccagtgcc taatgccagc attttcccgg ccagtaacgg accgctcata gcccccagac
     8101 gccctaccgc cacggctgtt cccacacctg ttgcgcggat ctgcgaactg taaaacaacg
     8161 gtgccagagc atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg acaaatcccg
     8221 ccagcaacat actgttaaat gacgacaccg ttcccagcgc cagcagcgaa gctaacatgc
     8281 cgctataaat cagtagcgac atggttactg gacgcagttt atccatcaat gcgcccaaca
     8341 ttaacgtccc gcttgccgcc cccatttgca gtgcaaacat cacccctgcc gcctgcgatg
     8401 gctggaatcc ttgctccacc aaaagcagcg gcagccagtt gatcaacatg tagaccacca
     8461 gcagagtgaa aaaataacac aaccacagca gcagcgtcgc ggttgccgtt tctggcgcaa
     8521 ataacgcacg cagtggtggc gcagcctgtt tttcgccagc gaaaaccgtc gactccggca
     8581 gccagcgcat taatagcggc accagaatca acggcaccac accacctacc caaaacaccg
     8641 tttgccatgc taagtttgcc cccgcgaaac ccagcgtcgc cgccagcgcc gcgccaatgg
     8701 gaacaccgca atacatcagg ctcactgccg tcccacgaaa acgtggtccc gcggcttcag
     8761 acgtcagggc gataagattc ggcaacgccg cccccagccc gacaccggtc atcagccgcg
     8821 caaagaccag tgaggggaaa tcccatgcaa tcgccgttgc cagcgagaac aaaccaaaca
     8881 gcgcaactga gccaatcaaa atacgcttac gcccataacg gtctgccagc atcccgccaa
     8941 ccagcgcgcc gggtagcaaa ccgagtattc cggcgctaaa tatccagccc atttgcattt
     9001 tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc tgaagatcca
     9061 gcccttccat cagagcgacc aaaaaacaaa gcccgatggt cagcatcagg cgggatgaag
     9121 atgatgaagg ggtacgagtc gacataagat gaacctcatt gcgagtgcag gtgaatcgcg
     9181 actgatacat tgccagccgc gtaatcagaa ctatttaatg actgcctacg ggcgaaatgt
     9241 aggccggata aggcgttcac gccgcatccg gcagtcgtgc tgggatgggc atacaaaatg
     9301 cacgttctcg gcagtcaata ttatttgttg ttgcgcagat ccagcgccac gtcgacgatc
     9361 atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat atccaccgcg
     9421 cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctgga gtaaacacca
     9481 gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gtcgatcctg caacggacgc
     9541 accaggtcgt cagcggcatc cattaacgca tagagatcgc ccccatgctg ccaacccagt
     9601 ttatccgcgg cggcaataaa tacttccagc ggcgcgttac ctgcgcccgc gcccattccc
     9661 gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga attcgccacg
     9721 ccaagactca ggttatggtg agcgtgcatg ccggtttgcg tttctggttt cagcactgct
     9781 ttcagggcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc agaatccacc
     9841 acataaatac aggtcgcacc gtagccttcc atcagctttg cctgctttgc gagattctcc
     9901 ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag ctcgcgggca
     9961 tactgaatat gctgggcgga aacatcagcc tcggtacagt gcgttgccac acgaaccacc
    10021 cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc tggcagcaac
    10081 aacgtcgcga ttttggcgtg cttcaccaca tccgccgccg cttcaatcca ttcaaggtcg
    10141 ctatgtgcgc cgaaaccata gttaaagctg gaaccctgca aaccgtcgcc gtgggccact
    10201 tcaatcgaat ccacgcgggc atcatccagt gctttggcaa tctggcggac attttccagc
    10261 gaatactgat gacgaatggc gtgcataccg tcacgcaacg tgacgtccga aatataaagt
    10321 tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc attttttccg
    10381 ctgtcgccag cgcactggaa gtcataatgt cgaggttgcc cgcataggca ggcagataat
    10441 gcgctgcgcc ttcgacttcc agccagaccg ccgttttcag cccggagaac tgccccacgc
    10501 ccggtaaatt gaccggttta tcctgcggga tgacttcaaa ctgcacgcgc tgtttcaggc
    10561 gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag gcttcgatat
    10621 catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc aacggtggct
    10681 ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccacccacc acttcaatgg
    10741 cgcgcgaagt ggtttccgta aattcatcga tgttggcacg cgtgccgggt ccggcagatt
    10801 tactggcaat agaagcgata atttcggcgt aatgaacacg cgccacgcgt gaaactgccg
    10861 ctaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc agttgagcga
    10921 cgttctcctc gaggttaacc accggcacgc agtaagggcc gatggcagca ggcgtcaggt
    10981 caattaagcg aatatccggt ttcgcttcgc gcaaagcggc atcgtttttc acatgcgcgc
    11041 ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg ttcatcagcc
    11101 cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc agaccgtcgg
    11161 actgaggatc aatgccgacc atcaccgcca tctccagatg ctgaccgtga cgcaaaattt
    11221 taatcatcag atcggtgcca atgttgcctg aaccgataat ggcgacttta cgcttactca
    11281 tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac ctatgccttc
    11341 gatatgggct tcaaaacgat cgcccgcatt caccgccacc atcggaccta atgccccggt
    11401 aagaatgata tctccggcgc gcagcggttc acccagactg gccattttgc gtgccagcca
    11461 gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg aaacctcttc
    11521 gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg ggcgttgcgc
    11581 cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat cgacaaactg
    11641 aatcgaccag tcgcgaatgc ggctccccac cacctccagc gccggaagta cccattcaat
    11701 ggcgttatac aattcgtcga aggtgatatc ggttgcgggt agatcgcggt tcaacaccag
    11761 tgcaatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatgg tttcattatc
    11821 gccataacac atgtcggcaa ataatgtccc gaaatccggt tgatcaacgc ccagttgttg
    11881 ctgcactttc ggatgcgtca gccccacttt gcgccccacc acgcgacgcc cctgcgcaac
    11941 gtcatgttgc acattgatgt gctgaatggc gtaggccgct tcagcgttat cgataccaat
    12001 cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta aatccgccgc
    12061 cagttgctca agagtatgct tcgtcatgac cgttccttaa gggcgtgcga ggaaattcag
    12121 caccagttga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac agtcgcggaa
    12181 gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat ccatcggcac
    12241 aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg ccagacgtgg
    12301 gccaaaatcc gggaactgtt tcggattggc ttccaggctc ttaacgaagt tttccaggtg
    12361 atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg tcaaatcgct
    12421 ggtatcaaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg gctgacgata
    12481 aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac tcatgccgcc
    12541 cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa gagtgaacgc
    12601 cacagaactg tggccaccca tcgagttgcc cagcaggtgg attttggcga tatccagttg
    12661 atccaccacg cttttcagga ttcgcgcatt aagatccgat cgcgaaccac tattaacgat
    12721 cgaatcgctc ttaccccaac ccggacaatc cagcaggatc acccgatagc ccgcctctac
    12781 cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc cggaaccatg
    12841 cagcaggaca acggtttcgt cgccttgtcc gcagtcatta aaatggatgc gcagcgtttt
    12901 acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg gctgataact
    12961 catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc aaatccggca
    13021 atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc aaacgcagaa
    13081 atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc ggcaatggcg
    13141 gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc cagcaaagtc
    13201 atgaactggt tatcccaaat cgggttgagc ggatgcagcg ttctctgatc ctcaacaaac
    13261 ttctcagcgg cgctaatcac ccgttgctga cgcaattcgc gctcactggc gggtaaatct
    13321 ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag ttcgggcacc
    13381 ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt gagagtactg
    13441 gtgaaacgtc caatggcttc ccccaacatg cgggtacgct ggaaaccggg cagcggcgtg
    13501 gcgacaccgt tgatgaacac cggcagtact ggcaccttat ccagcccacc gagcaggaac
    13561 tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc aagatcgatc
    13621 ccgctcttca tgacggcatg cgcacaggcc tccgccagct ccacaggcac gggcagctct
    13681 cctgccgcac tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca gaacggtggc
    13741 atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac cagttcaggg
    13801 gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc atcgagtacc
    13861 tcttgtgccg ggtcgacata ccccaccagc ggcgagtggg aaagacagtg aagataagcg
    13921 tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca gcgtcatcac
    13981 cgacgccagt ttattcaggg tcttgcccag ggtttgcgga atggcggtgg cggcaacaaa
    14041 gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc tacgcaggcg
    14101 accttgcgta tcccccacgc gtagtacgcc gtcgtggtta tcctgtgcgg tatgaatttg
    14161 cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct ggatttgctc
    14221 gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt tcgcgccgat
    14281 cgcgttatcg agtagcgtca cgtcgccgtt ttccagcgtg actttcggct gaataaacat
    14341 cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc cgtaatattg
    14401 cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca gataattcag
    14461 cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag ccagcacgtt
    14521 gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt cttgttgata
    14581 ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt tccatgcgag
    14641 gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg gcatgatgtg
    14701 cgcggcatcg cccgccagca gtacgcgatc aatacgaaaa cgttgcgcca gtcgcgcgtt
    14761 gtgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca gcactttgct
    14821 taacagcttg cgcatatttt gcggctcacg cagctgttct tcggtttctc ccggcatcac
    14881 cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg ggcgcaccgg
    14941 atcacaacac aaatagatat gcggcgtact taacggatcg ttggcgatat ctaccacaat
    15001 ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac ggacaaaact
    15061 tgccccacca tcacaggcca ccagccactg ggctttgacc gtttcccgct gcccttcttc
    15121 cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca gctcgcggga
    15181 aaacaggcag cgcacattcg gaaaacgcga caatccttcc agcatcaccg catcgacctg
    15241 aggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg gctgaatatc
    15301 agcaaaacag cgaccttttg gggtgagaaa acgcatcgcg tgccacggcg tagtgtgcgg
    15361 cagaacatta tcgaccaggc caaccgactg catggtgcgc agcgcctcgt catcaatacc
    15421 aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca cgtcaatgcc
    15481 catctggccg agatagttcg ccatcatcag cccgaccgga ccggcaccag cgatcgccac
    15541 ctgaacgcta tggttaacag caggctggat gtcagggtgt tgtattgcca tttcagtacc
    15601 tcacgactcg gacaaaatgc cgttgcgcgc acagtacagc gcaacttatt ttgttaaaaa
    15661 catgtaaatg attttttatt gtgcgctcag tataggaagg g
//
LOCUS       DAJOTB010000112        15278 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJOTB010000112 DAJOTB010000000
VERSION     DAJOTB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.112"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            77..1366
                     /gene="uraA"
                     /locus_tag="OHN28_003467"
     CDS             77..1366
                     /gene="uraA"
                     /locus_tag="OHN28_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCQ2298798.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            1416..2162
                     /gene="hda"
                     /locus_tag="OHN28_003468"
     CDS             1416..2162
                     /gene="hda"
                     /locus_tag="OHN28_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCQ2298799.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2300..2659)
                     /gene="arsC"
                     /locus_tag="OHN28_003469"
     CDS             complement(2300..2659)
                     /gene="arsC"
                     /locus_tag="OHN28_003469"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCQ2298800.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2680..4143)
                     /gene="bepA"
                     /locus_tag="OHN28_003470"
     CDS             complement(2680..4143)
                     /gene="bepA"
                     /locus_tag="OHN28_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCQ2298801.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4356..5417
                     /locus_tag="OHN28_003471"
     CDS             4356..5417
                     /locus_tag="OHN28_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2298802.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(5515..5985)
                     /gene="bcp"
                     /locus_tag="OHN28_003472"
     CDS             complement(5515..5985)
                     /gene="bcp"
                     /locus_tag="OHN28_003472"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCQ2298803.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(5985..6557)
                     /locus_tag="OHN28_003473"
     CDS             complement(5985..6557)
                     /locus_tag="OHN28_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCQ2298804.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            6703..7581
                     /gene="dapA"
                     /locus_tag="OHN28_003474"
     CDS             6703..7581
                     /gene="dapA"
                     /locus_tag="OHN28_003474"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCQ2298805.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            7598..8632
                     /gene="bamC"
                     /locus_tag="OHN28_003475"
     CDS             7598..8632
                     /gene="bamC"
                     /locus_tag="OHN28_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCQ2298806.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            8845..9558
                     /gene="purC"
                     /locus_tag="OHN28_003476"
     CDS             8845..9558
                     /gene="purC"
                     /locus_tag="OHN28_003476"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCQ2298807.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            9726..10589
                     /locus_tag="OHN28_003477"
     CDS             9726..10589
                     /locus_tag="OHN28_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCQ2298808.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            10604..12619
                     /gene="tmcA"
                     /locus_tag="OHN28_003478"
     CDS             10604..12619
                     /gene="tmcA"
                     /locus_tag="OHN28_003478"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCQ2298809.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            12693..13391
                     /gene="ypfH"
                     /locus_tag="OHN28_003479"
     CDS             12693..13391
                     /gene="ypfH"
                     /locus_tag="OHN28_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2298810.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(13472..13672)
                     /locus_tag="OHN28_003480"
     CDS             complement(13472..13672)
                     /locus_tag="OHN28_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCQ2298811.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(13700..14827)
                     /gene="dapE"
                     /locus_tag="OHN28_003481"
     CDS             complement(13700..14827)
                     /gene="dapE"
                     /locus_tag="OHN28_003481"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCQ2298812.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(14831..15187)
                     /locus_tag="OHN28_003482"
     CDS             complement(14831..15187)
                     /locus_tag="OHN28_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCQ2298813.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         3427 a         3814 c         4171 g         3866 t
ORIGIN      
        1 ataattaaag ccgactttaa gagtcggctt ttttttgagt aaagcgccta taacacataa
       61 tacagaggat aatactatga cgcgccgtgc tatcggggtg agtgaaagac cgccactttt
      121 acagacaatc ccgcttagtt tgcaacattt gttcgccatg tttggtgcaa ccgtcctggt
      181 gcccgtctta tttcatatta acccggcgac tgtactgtta tttaacggta taggaacact
      241 gctgtatctc ttcatctgta aagggaaaat tccggcttat cttggttcca gctttgcctt
      301 tatttcaccg gtattgttac tgttgccgtt ggggtatgaa gtcgcgctgg gcggctttat
      361 tatgtgcggc gtgctgttct gcctggtttc ttttatcgtg aagaaagcgg ggaccggctg
      421 gctggacgtg ctgtttccac ctgcggcaat gggcgcaatc gttgccgtca tcggtctgga
      481 gctggcgggc gtagctgccg gtatggcggg tttactcccg gctgaagggc aaacgccaga
      541 ctccaaaacc atcatcatct ctattaccac cctggcggtc acggttttag gttccgtgct
      601 gtttcgtggt ttcctggcaa ttatcccgat tttaattggc gtgctggtgg ggtacgcgct
      661 ctctttcgca atgggaattg tcgataccac gccgattatt aatgctcact ggtttgcgct
      721 gccgaccctc tatacgccgc gcttcgagtg gtttgccatt ctgactattc tgccagcggc
      781 gttagtggtt attgccgaac acgtagggca cctggtagta acggctaata tcgtcaaaaa
      841 agatctgctg cgcgatccag gtctgcaccg ttcgatgttt gctaacggct tgtcgaccgt
      901 gatctccggc ttctttggct ccacgccaaa caccacttac ggagaaaaca ttggcgtgat
      961 ggcgatcacc cgtgtttaca gtacctgggt tatcggcggt gcggcgattt tcgctatcct
     1021 gctttcctgc gtcggtaaac tggctgctgc tatccagatg attccattgc cggtgatggg
     1081 cggcgtttcg ctgctgcttt acggtgtcat cggtgcttcc ggtattcgtg tgttgatcga
     1141 atcgaaagtg gattacaaca aagcacagaa cctgatcctg acttccgtga ttttgatcat
     1201 cggcgtcagt ggcgcgaagg taaacattgg ggcggcggag ctgaaaggta tggcgctggc
     1261 gaccatcgtc ggtatcggct taagtttgat cttcaaactg attagtatgc tgcgccctga
     1321 agaagtggtg ctggacgcag aagacgcgga tataacagac aaataatgta ccgaccgggc
     1381 agtgttgctg cccggttcaa acatcatggg attctgtggt aaacttctcg cgattttgtg
     1441 aaatcctggt tgaggtatct ctgaacacac cggcacagct ctctttgcca ctttatcttc
     1501 ctgacgacga aacctttgca agtttctggc cgggggataa ctcctcttta ctggccgcgc
     1561 tgcaaaacgt gctgcgtcag gaacatagcg gttacatcta tctctgggca cgcgaaggcg
     1621 cggggcgcag ccatctgctg cacgcggctt gcgcggaatt gtcgcagcgt ggcgatgcgg
     1681 tgggctatgt cccgctggat aaacgcacct ggtttgttcc ggaagtgctc gacggtatgg
     1741 agcatttgtc gctggtctgt attgacaaca ttgagtgtat tgccggcgat gagttgtggg
     1801 agatggcgat tttcgatctc tacaatcgaa ttctggaatc gggcaaaaca cggttgttga
     1861 tcaccggcga tcgtccaccg cggcagttga atctgggatt accggatctc gcgtcgcgtc
     1921 tcgactgggg gcagatctac aaattgcagc cactttctga tgaagataag ttgcaggcgc
     1981 tacagttacg cgcgcgtttg cgtggttttg aactgccgga agatgtgggg cgtttcttgc
     2041 tgaagcggct cgacagagaa atgcgcacgt tgtttatgac gctggatcag ctggatcgtg
     2101 cgtcgattac cgcgcaacgc aagctgacca ttccttttgt gaaagaaatt ctgaagttgt
     2161 agataaggtg tttattgtcg gatgcggcgc gaacgcctta tccgacctac gcgaagttaa
     2221 tccatagtat tcgtaggccg gataagacgc ggcaagcgtc gcatccggca atgtgcttca
     2281 actagcgatg gagcacatct taacccacga tttccagtac ctgctccggt ggacgaccaa
     2341 tccgtgcttt gccattcgcg accacaatcg ggcgttccat tagtttcgga ttatccacca
     2401 tcgcctgaat cagcgcctct tcgctaagtg aactgtcagc caggttcagt tctttataaa
     2461 gatcctcttt ttggcgcatc aattcacgag cgctattcat ccccagcatt ttcagcaaat
     2521 cacgcagcgt tgccgcatcg gcgggtgtct caaggtagag caccacttct ggctccacgc
     2581 cgttttcttt cagcaggttc agcgtttccc ggctctttga acagcgtggg ttatggtaga
     2641 tttttacctg tttggtcatt gttcttcctt taatgcgaat tacatcttgg tataaggctt
     2701 aaagcgttcc tgcagctggc gcaactggtc gatgcgcgca tcgtaacgcg cttgttgcag
     2761 gctgcctaat ttcacctgcg aactggcgct actcaacaag gaaatagcct gatcgagtcg
     2821 cccggcgagc gcataacctt ctgctcgcgc agccagttcc tgatcgcggt tatttagtgc
     2881 ggcttccgcc tgtgccagta aatcccagcc gttgctgtca tctttattgt taaaagtgta
     2941 gcgattcaga atattggccg cttcctgtgg ttgcccccct tgcagataag cgttcgccag
     3001 gttgagctgc aacaccggat tggtgcgcaa atcgcgggca tttttcaggc gattgatagc
     3061 gtcattggct ttgttttgcc caagatcgat atcggtagcc agatcgagat accatgcgtt
     3121 gccaggttct gctgccagta acggttgcag cgttttacgc gcttcgtcgt atttattggc
     3181 ttccatcgcc tgtagagcac gaccatattg cgccgctcgt tgctgacgaa cgtttccttt
     3241 cgcccattca tccagcaaat cactggtgag ctggttacgt ccggaattat acatccccag
     3301 tgtgcgcgct ttcgccagat agaaatcttc cgacgactgc accaccatcg ggcgcatctg
     3361 attagcacgg ttgcgggcat ctgccagacg actttccggc aacgggtgag tcaataaaat
     3421 ttccggcgga cgcgaggagt aacgcgcctg atcgagtaat ttttcgagga aggtcggcat
     3481 cgcctgcgga tcgaatcccg agcgttgcag cacctgaata ccaatgcggt ccgcttcctg
     3541 ttcattttgc tgggtgaaac tgatcatccc ctggcgcgtt cccgccagtg taccggtcag
     3601 cgccgccatc cccgcctgcg gactggccat cgccagtaaa atagaaccta acgcgccgac
     3661 ccaggtcagc ggcgcgctgc gctgctgatc ttccatcgct cgcgccaggt gacgttgggt
     3721 gacgtgggag atttcgtgcg ccataactga agccagttga ctttcgttat cggaataacg
     3781 gaacagggca gagtgcagca ccacgttgcc gccaaagaaa gcaaaggcgt taatttcgtc
     3841 gttgttgatc agaaagaaat gaaacggtgt cttaaccgaa ttggcatgcg aaaccagacg
     3901 cattcctagc gaattaatat attgcgttaa tagcgggtca ttaattaacg gcgcgctgcc
     3961 gcgtaactgg cggacataat agtcgcccat ctgcatttcc tgaccaatgg aaagtgtgct
     4021 tcctgcggag gttcccatat ccggcaaggt gtctgcgctg tcggcaaacg ccggggctac
     4081 ctgaccaatg gtcatagcag caatgagggt tgcaaccagg ttttttttca actgcctgaa
     4141 cataacctct atcctgtatt tctggagata gtcatttgac cgatactgtg aaatatcgtt
     4201 cccaccgcgc cgcctttgag tgtaacgacg aagattagcc cactcaaata acttttcagt
     4261 ttcctgaatc cgtcataaaa agcgaagtct tttttgtgac atttcgatac aattgcggat
     4321 cgcaatcccg ctcttgaggt tcaggtaagg tctgtatgct cgaaatgttg atgcaatggt
     4381 atcgccgccg ttttagcgac ccggaagcga ttgccttgct ggttatttta gtcgccggat
     4441 ttggcattat ctttttcttt agtggcctgc ttgctccgtt gctggtggct attgtgctgg
     4501 cctatttgct ggagtggcca accgtgcgcc tgcaatctat tggctgttcc cgccgctggg
     4561 cgacgtcgat tgtattggtg gttttcgtcg gtatattgct actgatggcg ttcgtggttc
     4621 tgcctattgc ctggcagcag ggcatctact taatccgcga tatgccgggg atgctcaata
     4681 agctttctga ctttgccgcc acgttgccgc gccgctatcc ggcgttaatg gatgcgggca
     4741 ttattgatgc aatggccgaa aatatgcgca gtcggatgct gaccatgggc gattcggtgg
     4801 tgaaaatttc cctcgcctcg ttggtcggtt tgctgaccat tgccgtctat ctggtgctgg
     4861 tgccattgat ggtcttcttc ctgctgaaag acaaagagca gatgctgaac gccgttcgcc
     4921 gggtgctgcc gcgcaaccgt ggactggcag gacaggtgtg gaaggagatg aatcaacaaa
     4981 tcaccaacta tatccgcggc aaagtgctgg agatgatcgt ggtagggatc gccacctggc
     5041 tggggttctt gctctttggg ctgaactatt cgctgctgct ggcggtgctg gtcggcttct
     5101 cggtacttat tccgtacatt ggcgcatttg tggtgaccat tccggtggtt ggcgtggcgc
     5161 tattccagtt tggtgctggc acggaattct ggagctgctt cgcggtgtat ctgattattc
     5221 aggcgctgga cggcaacctg ttagtaccgg tgttgttctc cgaagcggtt aacctgcatc
     5281 ctctggtgat tattttatcg gtggtgatct tcggtggttt gtggggattc tggggcgtat
     5341 tcttcgctat tccattggcg acgctgatca aagccgtgat tcacgcctgg cctgatgggc
     5401 aaatcgcgca agactgacct tcagcgaaaa atttgaaacg agtgttgcag ggcagtgaga
     5461 agatttgaga tgtgctggcc gcaagcgcag ccagcacgga atggagcaaa gtaatcaggc
     5521 gtgttctttc agccagttca gcacaacgtc gtggtgattg ctggttttga aatcgtcaaa
     5581 gacatgttcg attttgccat cagcgtcaat caggaagctg atgcgatgaa taccatcgta
     5641 ggttttgccc atgaaggact tttcacccca gacgccgaat tgttcgcaca cctggtggtc
     5701 ctcatcagac aggagcgtaa agttaagcag ctctttttcc gcaaaacggg agagtttttc
     5761 gggtttatcg gtgctgatac ccagcacatc aacgcccgct tttttcaact catccatgtt
     5821 atcgcgtaag ccgcaggcct gtacggtaca gccgggggtc atggctttcg ggtagaaata
     5881 aaccagaaca cgctgtccct ggaagtcggt caaattaact tgttctccgt cttgatccgg
     5941 caagctaaat ttcggtgcga tatcaccggc tttcagtgga ttcattactt aactccatcc
     6001 tgttcatcat gttgggaata attgacgacg ttaatactgc cttgtgcatt gagttctgta
     6061 catagggctt tgaacgcttg ctcaatattt gctgcgtccg cagatgcggg gctgtgggca
     6121 gttatctgaa tatgtaactg cgcagcccgt tcattttcag caggttgcgt gcgcgacacc
     6181 agctccgcaa tgttcatatg atgcgcgtcg aaaagtgctg tgaagcgttc aattaaatgc
     6241 ggggagtctg ccacatcgac ctgaacccag acagatgctg gcattggtgg acgcggacgc
     6301 gccgtcgtgc gcttcatcac gattaaaaga tccagttcgg cacctttcaa cggtaacgtt
     6361 gattcaatca gggtgattgc attccatgaa ccggaaagca gcatgataaa cgtgaactct
     6421 tctcccagca tcgccaggcg actgtcttca atattacagc cgcaactact gacatgacgg
     6481 gtgatggtgt tcactattcc agggcgatcg gcacccaacg cagtgatcac cagataatgt
     6541 tgcgatgaca gtgtcaaact ggttattcct tcaaggggtg agttgttctt aaggaaagca
     6601 taaaaaaaac atgcatacaa caatcagaac ggttctgtct gcttgctttt aatgccatac
     6661 caaacgtacc attgagacac ttgtttgcac agaggatggc ccatgttcac gggaagtatt
     6721 gtcgcgattg ttactccgat ggatgaaaaa ggtaatgtct gtcgggctag cttgaaaaaa
     6781 ctgattgatt atcatgtcgc cagcggtact tcggcgatcg tttctgttgg caccactggc
     6841 gagtccgcta ccttaaatca tgacgaacat gctgatgtgg tgatgatgac gctggagctg
     6901 gctgacgggc gcattccggt gattgccggg accggtgcta acgctactgc ggaagccatt
     6961 agcctgacgc agcgcttcaa tgacagcggt atcgtcggct gcctgacggt aaccccttac
     7021 tacaatcgtc cgtcgcaaga aggtttgtat cagcatttca aagccatcgc tgaacatact
     7081 gacctgccgc aaattctgta taatgtgccg tcccgtactg gctgcgatct gctcccggaa
     7141 acggtgggcc gtctggcgaa agtaaaaaat attatcggaa tcaaagaggc aacagggaac
     7201 ttaacgcgtg taaaccagat caaagagctg gtttcagatg attttgttct gctgagcggc
     7261 gatgatgcga gcgcgctgga cttcatgcaa ttaggcggtc atggggttat ttccgttacg
     7321 gctaacgtcg cagcgcgtga tatggcccag atgtgcaaac tggcagcaga agggcatttt
     7381 gccgaggcac gcgttattaa tcagcgtctg atgccattac acaacaaact atttgtcgaa
     7441 cccaatccaa tcccggtgaa atgggcatgt aaggaactgg gtcttgtggc gaccgatacg
     7501 ctgcgcctgc caatgacacc aatcaccgac agtggtcgtg agacggtcag agcggcgctt
     7561 aagcatgccg gtttgctgta aagtttaggg agatttgatg gcttactctg ttcaaaagtc
     7621 gcgcctggca aaggttgcgg gtgtttcgct tgttttatta ctcgctgcct gtagttctga
     7681 ctcacgctat aagcgtcagg tcagtggtga tgaagcctac ctggaagcgg caccgcttgc
     7741 ggagcttcat gccccggctg gaatgatttt gccggtgacc tccggcgatt atgcaatccc
     7801 ggtgaccaac ggtagtggtg ctgtcggtaa ggcgctggac attcgtccac cagcccagcc
     7861 gttggcactg gtttctggcg cgcgtaccca gttcacgggc gataccgctt cattgctggt
     7921 ggaaaatggt cgtggcaata ctctgtggcc gcaggtggtt agcgtgctgc aggcgaaaaa
     7981 ctacaccatc acccaacgtg atgatgctgg tcagacactg accaccgatt gggtacaatg
     8041 gaaccgtctg gacgaagacg agcagtatcg tggtcgttat caaatctctg ttaagccgca
     8101 gggttatcag caggcggtta cggttaaact gctgaacctg gaacaggcgg gcaaaccggt
     8161 tgcagacgcg gcgtccatgc agcgttacag cacggagatg atgaacgtta tttccgccgg
     8221 tctggataaa tctgccactg acgccgcgaa cgctgcgcaa aatcgtgcct ccaccactat
     8281 ggacgtacaa agtgcagctg atgacaccgg tttaccaatg ctggtcgtac gcgggccgtt
     8341 caatgtggtt tggcaacgtc tgccagctgc gctggaaaaa gtgggcatga aagtgaccga
     8401 cagcacccgt tcgcagggca acatggccgt aacttataag ccgctgtctg acagcgaatg
     8461 gcacgaactg ggcgcgagcg atccaggtct ggcatccggt gactataaac tgcaggttgg
     8521 cgatttagat aaccgcagca gcctacagtt catcgatccg aaaggtcata ctctgactca
     8581 gagtcagaac gacgcgctgg tagctgtctt ccaggctgcg tttagcaagt aaaaatacag
     8641 ggctggagtc atccggccct tttttctgat atgatacgca aacgtgtgcg tcggcagaaa
     8701 aacgcgattt tagcggtaat tcgcacgaaa tttgtttgtc ggacgtaggt cggataaggc
     8761 gttcacgccg catccgacaa aacatccggc acaccagaca gcaaaagatt ttaaaacgtt
     8821 aattcacacc caggagtgat aaagatgcaa aagcaagctg agttgtatcg tggtaaagcg
     8881 aaaaccgtat acagcacgga aaacccggac ctgttggtgc tcgaattccg caatgatacg
     8941 tcagcagggg atggcgcgcg cattgagcag tttgatcgca aaggtatggt gaacaacaag
     9001 ttcaactact tcattatgag caaactggct gaagcgggta tcccgactca aatggagcgt
     9061 ctgctctccg ataccgaatg tctggtgaaa aagctggata tggtgccggt tgagtgtgtc
     9121 gtgcgtaacc gtgctgctgg ctctctggtg aaacgtcttg gaatcgaaga aggtattgag
     9181 ctgaacccgc cgctgttcga tctgttcctg aaaaacgacg ccatgcacga tccgatggtc
     9241 aacgaatctt actgcgaaac ctttggctgg gtgagcaaag agaacctggc gcgtatgaaa
     9301 gagctgacct acaaagcgaa cgacgtgctg aaaaaactgt tcgatgatgc tggtctgatt
     9361 ctggtcgact tcaagctgga gtttggtctg tacaaaggcg aagtggttct gggcgatgag
     9421 ttctccccgg acggcagccg cctgtgggac aaagaaacgc tggagaaaat ggacaaagac
     9481 cgtttccgcc agagcctcgg tggcctgatc gaagcctatg aagccgtcgc ccgccgcctg
     9541 ggtgtacagc tggactgatt tttctgttca tcatcttgcc gtgctgctgg cacggcaaga
     9601 caaccgctct cataagatga gtattgaacg ctcattcctc cgccatttca tcccgctttc
     9661 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat aatgttgagg
     9721 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaatagct
     9781 ccggcggtcc gtcaatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga
     9841 ttttactgat agtcgtgctg gttgcaggct actatggcgt tgatttaacc gggttgatga
     9901 ccggacagcc ggtttcccaa cagcaatcaa cgcggtcaat tagccctaat gaagacgaag
     9961 ccgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga cagcaattcg
    10021 agaagatggg taaaacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgta
    10081 ccggctgcgg ggcgggccag tccataatgg ggccattcta ttgcccggcg gatggcacag
    10141 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
    10201 ttgcccaggg gtacgttatc gctcatgaag tcggtcatca tgtgcagaaa ctgttaggca
    10261 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagta aaccgcttat
    10321 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc
    10381 agcaaggtgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
    10441 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt ttcactcatg
    10501 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
    10561 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
    10621 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
    10681 cggggaagag cgttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
    10741 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct cggcactaca
    10801 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
    10861 cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg tgttgttact
    10921 ccctgtatgg gatgagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg
    10981 ccctgaccct attgcgacgc cgcattttgt ccagcatttc aaacgcgtac ttacggcgaa
    11041 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
    11101 tactgactgg caccccgcta ccggcgcacc acaaccagaa caacagcaac tcttacagca
    11161 gctactgacc atgccgccgg gtgtggcagc ggtaactgct gcgcgtgggc gcggtaagtc
    11221 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgctcccgc
    11281 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcgcc
    11341 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg aagccgcagc
    11401 tatacctgcg ccgttgttgt atcaactggt atcgcgtttt cctcgaacgt tgttaactac
    11461 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
    11521 tccgcattta caccgttttg aactacaaca gccgatccgt tgggcgcagg gatgcccgct
    11581 ggaaaaaatg gttagtgagg cactggtgtt tgacgatgaa aacttcaccc atacaccaca
    11641 aggcaatatc gttatttccg catttgaaca gacgttatgg cgaagcgagc cagaaacgcc
    11701 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc tggatttacg
    11761 ccgcatgatg gatgcaccag gtcaacattt tttacaggcg gctggcggaa acgagattgc
    11821 cggagcgctg tggctggtgg atgagggcgg attatctcaa gaactcagtc aggcggtatg
    11881 ggcgggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggcag
    11941 caatccactg gcggcgacat tgcgcggacg gcgggttagc cggatagcag ttcatccgac
    12001 tcgtcagcgg gaaggcacag ggcgacagct tattgctggt gctttgcaat atattcatga
    12061 cctcgactat ctttcggtga gttttggtta caccgaggag ttatggcgtt tctggcaacg
    12121 ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg gttgctatac
    12181 ggcgatggcg ctgttaccga tgagtgatgc gggtaaacag ctggctgaac gtgagcatta
    12241 ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaatgc ttcctgttga
    12301 tccactaaac gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt
    12361 cactcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac aaaccagcga
    12421 actggcatta ccggcgctgc gtgggcgttt acagaaaaac gccagtgacg cgcagttatg
    12481 taccacactt aaactttcag gccgtaagct gttactggtt cgccagcggg aagaggccgc
    12541 gcaggcgcta ttcgcacttg atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
    12601 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg
    12661 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
    12721 gcccggataa accagcacaa cagttgttac tgcttttcca cggtgtcggg gataacccgg
    12781 tggcaatggg ggaaataggt agctggtttg cgccgctgtt tcctgatgcg ctggtggtga
    12841 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg
    12901 gtattacgga agataatcgc caggcgcgtg tgaatgccat catgccgacg tttattgaga
    12961 cggtgcgcta ctggcagaaa caaagcggag taggggctaa cgccacggcg ctaatcggtt
    13021 tctcacaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc
    13081 gcgtcattgc cttcaacgga cgctacgcga gcctgccgga aaccgcgtct acagccacaa
    13141 caattcactt gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg
    13201 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
    13261 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
    13321 cgaaacatta tttcgacgaa gcgctaagcg gcggtaagcc tggcgatgat gatgtgattg
    13381 aaatgatgta ataatagccg ggtaggtctg ataagactgt atcaggcatc atggccggat
    13441 gcggcgttaa cgccttatcc ggccacttag cttacttctt cggttgatcc tttttcggcc
    13501 agtcgtcgtc atcgtcccac ttatcgttga aatcacgatg cgggggaagc tccggcttgt
    13561 tggcgagaaa tttcttatgg tcgacgcgct tgagatcttt aatcacgttc agcaggacgc
    13621 ctaccaaaaa gacaatcacc agaatccacc aatatttagc cagccagtcc atgcttattt
    13681 cctcttgcag aaccactcat caggcgacga gctgttccat gatacgttga tacatacggg
    13741 caagtagctg caggtcggca gcgttcacac attcattgat tttatgaatc gtggcattga
    13801 ccggcccgag ttccaccacc tgcgccccca tgcgggcaat aaagcgcccg tcggacgttc
    13861 cgcctgtggt cagtagctgc ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg
    13921 catccaccag tttaccgcgc gcggtcaaaa atggctgccc ggaaagccac caatccaccg
    13981 tatagcgcag ttgatgtttt tcaagcaggg caagcacctg cgctttgatc atctcatccg
    14041 tcagttcggt gctaaagcgg aagttaaact gcacaaacag ttcacctgga ataacgttgt
    14101 tactgcccgt tcccgcctga atattggcaa tctgcatact ggtcgccggg aagaagtcat
    14161 taccctgatc ccactcaata gccactaatt cattaatgaa aggtgctgcg cgatgtaccg
    14221 gattgtcagc cagatgtggg taggcaacat gcccctgaac gccatgaatg gtaaggttgc
    14281 aggttaacga tccgcgacga ccatttttca ccacatcacc taccacttcg atactcgacg
    14341 gttcgccaac cagacagtaa tcgagacgct cattacgcgc cattaacgct tcgacgactt
    14401 ttaccgtacc gttgtgggca ctagcttctt catcagaggt gatcagaaat gccagtcgcc
    14461 ctgtatggtt gggatgttgt gcgacaaagc gttctgccgc taccaccatc gccgccagcg
    14521 agcctttcat atctgccgca cctcgcccaa ataacatgcc gtcacgaatg gttggttcaa
    14581 atggtggatt gatccaacgg tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa
    14641 aggctaacgt ttcaccctgc ccgcgccatg cccaaaaatt ctgcgtatcg gcaaagtcca
    14701 tgcgttcaac ggtaaaaccg atcgcctgca aacgttcaat caacaaagcc tggcatcctg
    14761 cgtcatcagg actcagggaa gggcggcgaa taagctgttg tgtcagctca ataaccgggc
    14821 acgacataga ctacacctca tggaaaaatt gctgataact ggattcactg aaacccagca
    14881 gcataggctt accaggcgcg cagagcaatg gacgtttgat aattgcaggc atttcagtca
    14941 ttaatgtcgc cgcagaggcc gcatcggtga ttttattgcg ggtggtttcg tccagtttac
    15001 gccaggttgt accacgggtg ttgagtaacg cttcccagcc taattcgttg ataaaaccgt
    15061 tcaataattc gctgtccaat ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat
    15121 tggcttccag ccaacggcga gcctttttaa tggtgtcaca atttttgatg ccgtaaagtg
    15181 taaccataat gaatcctttt tcgatttatg agaataaata ctcatttaag ggtgaaaaca
    15241 ttttgcctgc aaatgattat caggaaatat aacattat
//
LOCUS       DAJOTB010000113        15252 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJOTB010000113 DAJOTB010000000
VERSION     DAJOTB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.113"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..362
                     /locus_tag="OHN28_003483"
     CDS             <1..362
                     /locus_tag="OHN28_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCQ2298814.1"
                     /translation="IDELSRLLFVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRV
                     SLLTHTLILLLCLGLAWGAIEKTAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELR
                     HLYRQFTNTPSRNQQGA"
     gene            365..1642
                     /gene="yiaN"
                     /locus_tag="OHN28_003484"
     CDS             365..1642
                     /gene="yiaN"
                     /locus_tag="OHN28_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCQ2298815.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1655..2641
                     /gene="yiaO"
                     /locus_tag="OHN28_003485"
     CDS             1655..2641
                     /gene="yiaO"
                     /locus_tag="OHN28_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCQ2298816.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2645..4141
                     /gene="lyxK"
                     /locus_tag="OHN28_003486"
     CDS             2645..4141
                     /gene="lyxK"
                     /locus_tag="OHN28_003486"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCQ2298817.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4138..4800
                     /gene="ulaD"
                     /locus_tag="OHN28_003487"
     CDS             4138..4800
                     /gene="ulaD"
                     /locus_tag="OHN28_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2298818.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            4793..5653
                     /locus_tag="OHN28_003488"
     CDS             4793..5653
                     /locus_tag="OHN28_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCQ2298819.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMLETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5647..6342
                     /gene="araD"
                     /locus_tag="OHN28_003489"
     CDS             5647..6342
                     /gene="araD"
                     /locus_tag="OHN28_003489"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2298820.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6374..7189)
                     /locus_tag="OHN28_003490"
     CDS             complement(6374..7189)
                     /locus_tag="OHN28_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2298821.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            7415..8815
                     /locus_tag="OHN28_003491"
     CDS             7415..8815
                     /locus_tag="OHN28_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2298822.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLVVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            8826..10799
                     /locus_tag="OHN28_003492"
     CDS             8826..10799
                     /locus_tag="OHN28_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCQ2298823.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQVDN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(10976..12514)
                     /gene="aldB"
                     /locus_tag="OHN28_003493"
     CDS             complement(10976..12514)
                     /gene="aldB"
                     /locus_tag="OHN28_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCQ2298824.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSYGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(12622..13917)
                     /locus_tag="OHN28_003494"
     CDS             complement(12622..13917)
                     /locus_tag="OHN28_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCQ2298825.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(14047..15198)
                     /gene="yiaY"
                     /locus_tag="OHN28_003495"
     CDS             complement(14047..15198)
                     /gene="yiaY"
                     /locus_tag="OHN28_003495"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCQ2298826.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         3502 a         4082 c         3992 g         3676 t
ORIGIN      
        1 cgattgatga attatcccga cttttatttg tctggttaac ctttattggc gcaatcgttg
       61 cttatatgga caacagccat gtgcaggtta cttttttagt cgaaaaactc tcaccggcta
      121 atcaacaacg tgtttcgctg ctaacccata cgctcatttt attgctgtgt ctggggctgg
      181 cctggggcgc tatagaaaaa acagcgcaag actggagcaa cttatcgccc attcttggcg
      241 tccccgttgg cttaatgtac gccgccgcta tccccaccag tctgattatc gcattgctgg
      301 aattgcgtca cttataccgg cagttcacaa atacccccag ccgaaaccaa caaggagcct
      361 gacgatgaca atccttatat ttattgtcag tttgttaggg gctatcgcaa ttggtgttcc
      421 catcgcctgg gcattattgt tgtgcggcat ctcattgatg ttctggatgg atatctttga
      481 tgtgcaaatt ctggcacaaa cgttggtaaa tggcgcagac agcttctccc tgttagccat
      541 ccccttcttt gtactggcgg gtgagatcat gaacgcgggc ggcctgtcgc aacgtatcgt
      601 cgacttgcca atgaaactgg taggccacag gccaggcggt ctgggttacg tgggcgtgct
      661 ggcagcgatg atcatggcaa gcctgtccgg ttctgcggtt gctgataccg cagctgtcgc
      721 ggcgctgctg gtgccgatga tgcgccaggc caactatccg gtaaaccgcg ccgccgggct
      781 tatcggttcc ggcgggatta ttgcggccat tatcccacct tccataccac tcattatctt
      841 cggcgtctcc agcggactct ccatcagtaa gctgtttatg gcgggcatcg caccgggcat
      901 catgatgggc gtcacgctga tggttacctg gtggtggcag gcaaaacgac tgaatttacc
      961 ttgtcagccg aaagcctcac tgcgagaagt ctggcaatcc ttagtttccg gtatttgggc
     1021 gctcttttta ccgataatta ttattggcgg tttccgttcg gggttattta caccgaccga
     1081 agcaggcgcg gttgccgcct tctacgctct gttcgtctcg gtggtggtct atcgcgagat
     1141 gaccttctcc acgctttatc atgtgctgat taacgcggca aaaaccacat ccgtggtgat
     1201 gttcctggtg gcctctgctg cggtatctgc atggcttatt acgatcgccg aattacccat
     1261 gatggtatcg gagctattac aaccgttggt tgattcgcct cgcttattat tcatcgtcat
     1321 tatgctcgct attatggtga tcagtacggt catggactta acgcctaccg tattaattct
     1381 tacaccagta ttaatgccat tagtgaaaga agcgggaata gatccggtct atttcggcat
     1441 catgtttatt atcaactgtt caatatcact gattacgcct ccagtcggta atgttcttaa
     1501 cgtggtatgc ggcgtggcga aacttaaatt tgacgatgcg gtaaaaggcg ttgcgcctta
     1561 cgtcatggtt ttattcatgt tgttagcatt atttattttt ataccggagc tcattaccgc
     1621 tcctcttaaa tggatgagtt aaaaggaaat cataatgaaa ttacgttctg taacctacgc
     1681 tttattaatt gccggagtgg cgacgttcag cacatcttct ctggcggcac aatcattacg
     1741 tttcggttat gaaacctcac aaaccgattc acaacatatt gcggcgaaaa aatttaatga
     1801 attactgcag gaaaaaacca aaggcgaact gaaattaaaa ctgttcccgg atagcaccct
     1861 tggtaatgcg caggcgatga tcagcggcgt gcgtggcggc actattgata tggagatgtc
     1921 tggctctaac aactttacgg gactctcgcc cgtcatcaac ctgctcgatg tcccgttcct
     1981 gttccgcgat accgctcacg cgcataaaac gctcgacggc aaagtcggtg atgacctgaa
     2041 agtctccctt gaaggtaaag ggctgaaagt gctggcttac tgggaaaacg gctggcgcga
     2101 tgtcaccaac tcacgccagc cagttaaaac acctgccgac ctgaaaggac taaaaatccg
     2161 caccaacaac agcccgatga atatcgccgc atttaaagtt tttggcgcta acccgatccc
     2221 gatgccgttt gccgaagtct ataccgggct ggaaacccgc actatcgacg ctcaggaaca
     2281 cccgatcaac gtcgtctggt cagcaaaatt tttcgaagtg cagaagtacc tttctctgac
     2341 ccatcacgcc tattccccac ttctggtggt gatcaacaaa gcgaagtttg atggcttaac
     2401 cccggagttc cagcaggcac tgatctcatc tgcgcaggaa gcgggtagct atcagcgcaa
     2461 actggtcgcc gaagatcagc aaaaaatcat cgacggtatg aaagaagcgg gcgtggaagt
     2521 catcaccgat ctcgaccgca aagcctttag cgatgcgctg ggtacccagg tccgtgacat
     2581 gtttgttaaa gatgttcccc agggtgctga tctgctgaaa gccgtggatg aggtgcaata
     2641 aaccatgacg caatactggc tggggttaga ttgtggcggt agctggctga aagccgggct
     2701 gtatgaccga gaaggccggg aggcgggcgt gcagcgcctg ccgctgtgcg cattaagtcc
     2761 acagccaggc tgggcagagc gcgacatggc agaactgtgg caatgttgca ctgctgtcat
     2821 tcgctccctg cttacccatt ctggcgtcag cggggaacaa attgtcggta tcggcatctc
     2881 cgcgcaggga aagggcttat ttctacttga taaaaacgac aaaccgctcg gtaacgccat
     2941 tttgtcctcg gatcgccggg cgatggaaat cgttcgtcgc tggcaggaag atggcatccc
     3001 ggaaaaactc tatccgctga cccgacaaac cttatggacc gggcatccgg tgtcgctgtt
     3061 acgctggctg aaagagcacg aaccagaacg ctacgcgcaa attggctgcg tgatgatgac
     3121 gcacgactac ctgcgctggt gtttaaccgg cgtcaaaggt tgcgaagaga gcaatatttc
     3181 tgagtccaat ctctacaaca tgagtcgcgg ggaatatgac ccgtgcctaa ccgactggct
     3241 ggggatcgct gaaatcaatc acgccctgcc gcctgttgtc ggatctgccg aaatctgcgg
     3301 ggaaatcacc gctcagacag ccgtactgac tggtctgaaa gcgggtaccc ccgttgtcgg
     3361 cggcctgttt gatgtggttt ccaccgcact ctgcgccggg ctcgaagacg aattcaccct
     3421 caatgcggtg atggggacct gggcggtgac cagcggcata acccacggtt tacgtgacgg
     3481 tgaagcgcat ccgtatgtct atggtcgcta cgttaacgat ggtcaattta tcgttcacga
     3541 agccagcccc acctcttccg gcaatctcga atggtttacc gcacagtggg gagaaatctc
     3601 atttgctgag attaatcagg ccgtagccag tttgccgaaa gccggtggcg ctctcttttt
     3661 cctgccgttc ctgtacggca gcaacgccgg actcgaaatg accagcggtt tctacgggat
     3721 gcaggccatt cacacccgtg cacacctctt acaggctatc tatgaagggg tggtgttcag
     3781 ccatatgacc catctcaacc ggatgcgcga acgttttact gatgtgcaca ccctgcgcgt
     3841 cactggcggc ccggcgcact ccgatgtctg gatgcaaatg ctggctgacg tcagcggtct
     3901 gcgtatcgag ctgccgcagg tggaagaaac cggctgcttt ggtgcggcac ttgccgcccg
     3961 cgtcggtacc agggtttatc gcgatttcag cgaagcccaa cgtgacttac agcacccggt
     4021 acgcaccctg ctgccggata tggccgccca tcagctttac caacaaaaat accaacgcta
     4081 tcaacatctc attgccgcac ttcagggtta tcacgcccgc atcaaggagc acacattatg
     4141 agtcgaccac ttctgcaact ggccctcgac cactcatcac ttgaagccgc gcagcgcgac
     4201 gtgacgctgt taaaagacag cgtcgatatt gtcgaagcag gcaccattct ctgtttaaac
     4261 gaagggcttg gcgcagtgaa agccttgcgc gaacagtgcc cggacaaaat catcgttgcc
     4321 gactggaagg tcgccgacgc gggtgaaacg ctcgcgcaac aggcatttgg cgcaggcgct
     4381 aactggatga ccatcatctg cgccgcgccg ctcgccacgg tggaaaaagg ccacgcgatg
     4441 gcacagaact gcggcggtga aattcagatc gagctattcg gcaactggac gctggacgat
     4501 gcccgtgact ggcatcgtat tggtgtacga caggcgattt atcatcgtgg tcgtgacgcg
     4561 caggccagcg ggcaacagtg gggcgaggcc gatctggcac gcatgaaggc gctttccgat
     4621 atcggccttg agctttccat taccggcggg attacccctg ctgacctgcc gctgtttaaa
     4681 gagatccgcg tgaaagcgtt tattgccgga cggtcgctgg caggcgcagc aaatccggcg
     4741 caagtggccg ctgattttca tgcgcaaatc gactccatct ggggaggcaa gcgtgcgtaa
     4801 tcatccgtta ggtatttatg aaaaagcgct ggcgaaagat ctctcctggc cggagcggct
     4861 ggtgctggca aaaagctgtg gttttgattt tgtcgaaatg tcggtggatg aaaccgacga
     4921 acggttgtca cgtcttgact ggagcgccgc acaacgtgct tcgctggtca ctgcgatgct
     4981 cgaaacgggt gttgctattc cgtcaatgtg cctgtccgca catcgtcgtt ttccttttgg
     5041 tagccgggac gacgccgtgc gccagcgggc gcgggagatt atgagtaaag cgattcgcct
     5101 ggcacgcgat ctcggcattc gtactattca actggcaggt tacgacgtct attacgaaga
     5161 tcatgacgaa ggcacccagc aacggtttgc tgaagggctg gcgtgggcgg ttgaacaggc
     5221 agcggcatca caagtcatgc tggcggtgga gattatggac accgcgttta tgaactctat
     5281 cagcaaatgg aaaaagtggg acgaaatgct tgcgtcaccg tggttcaccg tttacccgga
     5341 cgtcggcaat ctcagcgcct ggggcaatga tgttcccgcc gaactgaaac tgggcatcga
     5401 ccgtatcgcc gccatccacc tgaaagacac tcaacccgtt accgaacata gccccggaca
     5461 gttccgcgat gtgccgtttg gcgaaggctg tgtcgatttt gttggcatct tcaaaacgct
     5521 gcatgagctg aactatcgcg gttctttcct gattgagatg tggaccgaaa aagccaaaga
     5581 gccggtgctg gagattattc aggcgcggcg ctggattgaa gcgcgtatgc aggaggccgg
     5641 atttatatgt tagagcaact gaaagctgac gtgctggcgg caaacctggc gcttcccgtt
     5701 catcatctgg tgacgttcac ctggggcaat gtcagcgcga tagacgaaac gcggcaatgg
     5761 atggtgatta agccttccgg cgttgagtat gaggtaatga ccgccgacga tatggtggtg
     5821 gttgagatag ccagcggcaa ggtggtggaa ggcagcaaaa agccctcttc cgacacgcca
     5881 acgcatctgg cgctttaccg tcgctttgcg gagattggcg gcatagtgca tacccactcg
     5941 cgccacgcca ccatctggtc gcaggccgga cttgatctcc ccgcctgggg cactactcac
     6001 gccgattatt tttacggagc catcccctgc acgcgcctga tgaccgcaga agagatcaac
     6061 ggtgagtatg aataccagac cggcgaagtg atcattaaaa ccttcgaaga gcgtgggttg
     6121 aacccggcac aaatcccggc ggtactggtg cattctcacg gcccgttcgc ctggggaaaa
     6181 aatgccgccg atgcggtgca taacgccgtg gtactggaag agtgcgccta tatgggtctg
     6241 ttctcgcgcc agcttgcgcc acagctccct gcgatgcaaa acgaactgct ggataagcac
     6301 tacctgcgta agcatggggc caatgcctat tacgggcagt aatccctcac gccggggctt
     6361 catcgccccg gcactacgaa ttgatatgtt ccttgctgta acgccgcttc cacgctgctg
     6421 gcgttaagcc ggtgtgtttg cgaaaaatct gccggaaata gccgacgtca ttaaacccgc
     6481 acaacgccgc cacttccgtg agcgatcgcg cttcgctaat taacagcttt tcggcttcgc
     6541 gcacccgctg gcggtggatt gcttctgtta acgtcagatg aaacacccgc cgatagacgc
     6601 gcccaagata atcggcattg cagtacagct cgcgggctaa aagcgccgtg gaaagcgggc
     6661 ggtgatattg cgtgcgaatc agtttctgcg ctttccaggc aagggtggtg cctgcaccat
     6721 cggcctgttc atcaagacgc gcgtcacggg tgagctgctg caaaatcagc agcacaataa
     6781 actccagcgc cggagtgcgt tgatgctttt cctgttcgct taagaactgg cgaaacaagg
     6841 ccaccagcac ctgcggatcg gcaaccttag tttgttgcgg cacgctaaga tgagtgagcc
     6901 agcgcggatc gttgtccggc gctttaacct caaaatgcag ccagtaaaat ttgagatcgg
     6961 cgggaaaatc ctccacccca acatgctgtc ggttcggcca gagtaataaa ctctctcccg
     7021 catcgaccag aaatatccgt tcatcttcct gaatcgttaa tcgacctttt tcgacgaaga
     7081 taatttccca tgactccagc cgccttgcag gatgcctccc tacgccacgg gaaataaata
     7141 atccaccatt ttgcacttta atcggtaacg tcatggataa ttcgagcatt gatatgtaat
     7201 ttcccgctta ttcattaatg attaaacaca ttaccttttg attattaatc aaaaacatta
     7261 atttcacctg atccatttca cacttttaaa ttcaggtcgg aatcgtcacc tttttttact
     7321 cttttctcgc cttgttgata ttcccgttca cggcagaatt aatcaggtca ttgcaataaa
     7381 actaaaaatt gctccctatt cacgaggaat atctatgacc tctacaccga ttaccaccgc
     7441 tgatatcgct aaaggtaaaa ttgacgatgc gttatctgta cgggaaaaaa taggctacgg
     7501 cctgggtgac gcaggcggca ccgtaataac ttgcctgatc atgaactttc tcaccttttt
     7561 ctacaccgac gtttttggct taactccggc gctggttggc acgctgttta ttgcgctacg
     7621 cgtgtttgat gccatctccg acccggtgat gggcgtcatt gccgaccgga cgcaaagtcg
     7681 ctgggggcgc tttcgtccgt ggcagttatg gattgccatt cccatcggca ttatcggcat
     7741 cctgacattc accgtgccag atgccagcat gggagtaaaa atcgcctggg cgttcggtac
     7801 ttacctgctc ctttcagtcg gttatacagc catcaacgta ccgtactgcg cgctgatcaa
     7861 caccatgacc acccgccaca atgaagtgat ctcctgccag tcctggcgat tcgttctctg
     7921 cggcgtagcg ggatttctgg tttcggtagg cttaccgtgg atggtagatc tcttcggtca
     7981 gggcaacgct gcacgcggct atcaacttgg cgtcggcgta ttgtgcgcca ttgccgtggt
     8041 gatgttcctg tgctgtttct tctgggttcg tgaacgggtg ccactctcca caatggggaa
     8101 atttaccctg cgcgaacatc ttgccgggct gcggaacaac gaccaactgc tgctgatgct
     8161 ggtcatgtct ttcctgctga ttaacgtctt taatattcgc ggcggtgggt atatgtactt
     8221 cattacctac gtcttacaag gcagtacggg ctacacgtcg ctgttcttca ccatggtcac
     8281 cttcgcctcc attatcggct cggtgattgt cagcccatta acgcggcgtt tcgataccgt
     8341 caaaatttac tactacacca acctgctcct cgctgcactg gcggtgttga tgtggttcct
     8401 gccctccagc ccggcttatc aaacgctgtg gctggtggtg atcctcggta atggcgtgat
     8461 tcttggcttc acattgccac tgcacttctc attgatggcc tttgccgatg actacggcga
     8521 gtggaaaacc cgcgtacgtt cttccggcat gaacttcgcc ttcaatctgt ttttcatcaa
     8581 gctggcctgg gcctccagcg ccgggatcat cagcctgctg tttatttttg tcgcctacca
     8641 gccaggcgtg gaaaaccaga ccgccagttc gcttggcggg atcgcggcga tggaaacatt
     8701 gctgcctgcg ctgttccacc tgctgctggc gatggcgatc cgcttttgca aactcaataa
     8761 ccctatgatg tcacgcattg ctagcgacct gcgtcagcgt catgtacagc cttaaggagc
     8821 acaagatgaa catttcggaa gtcgatctgc ataaactgac ggtcagcgat ccgttcctcg
     8881 gtcagtacca acaactggtc cgcgacgtgg tgattcctta tcagtgggat gccttgaacg
     8941 atcgtatccc agaagcggaa cccagccatg cgattgaaaa ctttcgcatt gccgccggac
     9001 ttcaggaggg tgaattttac gggatggtgt ttcaggacag cgacgtcgcc aaatggctgg
     9061 aagcggtagc ctggtcgctg tgccagaagc cggacgccga actggaaaaa accgccgacg
     9121 aggtgattga actgatcgct tccgcccagt gcgaagatgg ctatctcaat acttacttta
     9181 cggtaaaagc gcccgaagaa cgctggagca atctggcgga gtgtcatgaa ctttactgcg
     9241 ccggtcatct gattgaagcc ggagtcgcct tcttccaggc tacgggcaag cggcgcttgc
     9301 tggaagtggt ttgccgtctg accgatcata tcgacagcgt atttggtcca gatgaaagta
     9361 agttacatgg ttatcctggt cacccggaaa ttgaactggc actaatgcgc ctgtatgaag
     9421 tgaccgaaga gccgcgctac ctggcgctga cgaactattt tgtcgaacag cgtggtgcgc
     9481 aaccgcacta ttacgaccag gaatatgaaa agcgcgggca gacctcgcac tggcacacct
     9541 acggcccggc gtggatggtg aaagacaaag cctacagcca ggcacatttg cccatcgcac
     9601 agcagcaaac cgccattggt cacgcggtac gttttgtcta cctgatgacc ggcgtcgcgc
     9661 atctcgcgcg tttaagtcac gatgaaagca agcgtcagga ctgcctgcgg ctgtggaaca
     9721 atatggccca gcgtcagtta tatattaccg gcggcattgg ctcacagagc agcggtgaag
     9781 cgttcagcag cgattacgat ctgccgaatg acacggtata cgctgaaagt tgcgcctcca
     9841 tcggcctgat gatgtttgcc cgacgaatgc tggaaatgga aggcgacagt caatatgccg
     9901 atgtgatgga gcgcgcactg tacaacaccg tgctcggcgg catggcgctg gatggcaaac
     9961 atttcttcta tgtgaatccg ctggaagtac atccaaaatc gctgaaattc aaccatatct
    10021 acgatcacgt taagccgatc cgccagcgtt ggtttggctg cgcttgttgt ccgccaaata
    10081 tcgcccgcgt gctaacctcg attggtcatt atctctacac gccgcgtgaa gatgcgttgt
    10141 atatcaacat ctacgcagga aacagcatgg aggtgccggt agaaaatggc acgctgcgcc
    10201 tgcgggttag cggaaactat ccgtggcagg aacaggtgac gattgcggtt gaatcacccc
    10261 agccggtgcg tcatacgctg gctttacgtc taccggactg gtgcatacag ccgcagatca
    10321 tattgaatgg ggaagaggtc gagcaggata ttcgtaaagg gtatttgcac attacccgtg
    10381 aatggcagga gggcgacacg ctgaatctga ctttgccaat gccggtacgt cgcgtttacg
    10441 gtaacccgct ggtgcgtcac gtcgccggaa aagtggcgat tcagcgcggc ccgctggtgt
    10501 attgtctgga acaggtcgac aacggcgagt cactgcataa tctgtggctg cccgccgatg
    10561 cgccatttac gacatttgaa ggtaatggat tgttccgcca taagatctta atccaggcac
    10621 cgggttaccg gtatgaacag agcaatccag agcagcaacc gctgtggcat tacgacagcg
    10681 cgccagccaa acgccagccg caaattctga cgtttatccc gtggtttagc tgggccaacc
    10741 ggggtgaagg cgaaatgcgg atctgggtga atgaggaaac cagtgccatc cgtaggtagg
    10801 ataaggcgct cgcgccgcat ccaacgcatg gtgcacattg ccggatgcgg cgtgaacgct
    10861 ttatccgacc tacaaaagca tgcaaattca atatattgca tgagctatgt aggcatgata
    10921 agacgcgaaa gcgtcgcatc aggcaatgaa tacccgatgc gaccagcttc ttacatcaga
    10981 acagccccaa cggtttatcc gagtagctca ccagcaggca cttggtttgc tggtaatgct
    11041 caagcatcat tttgtgggtt tcacggccaa tgcctgattg tttgtagccg ccaaacgccg
    11101 catgtgccgg gtaagcgtga taacagttgg tccacacgcg ccctgcctga atgccgcgcc
    11161 ccatcttata ggccagatta ccgttgcggc tccagacgcc cgcgcccaga ccatattgcg
    11221 tatcgttcgc cagctccagc gcttcttcca tcgttttgaa ggtggttacc gccagcaccg
    11281 ggccaaaaat ctcctcctgg aacacgcgca tattgttctg accaaacaga atcgtcggtt
    11341 cgaggtagta gccgtctttc agttcgcctt ccagcagctt acgtcgcccg ccggtgagca
    11401 catcagcgcc ctctttttta ccgatatcaa tgtagttgag gatggtttcc agttgcccgt
    11461 aagaaacctg cgcgcccatt tgcgtcacgc tgtcgagcgg gttaccgctg cgaatgcttt
    11521 cgacacggcg gatagcgcgt tccataaagc gttcgtagat agactcctgc actaaagcac
    11581 ggcttggaca ggtgcaaact tcgccctggt taaaggcaaa cagtgcaaag ccttccagcg
    11641 ctttatcgaa aaaggcatct tcttcatcca tcacatcagc aaagaagata tttggcgatt
    11701 tgccgcccag ctccagcgtc accggaataa tgttttgcgt ggcgtattgc ataatttgtt
    11761 ggcccacttc cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat
    11821 attcgccaat ttccccacct gcgccattga ccacgttcac cacccctggc ggcagtaaat
    11881 caccgacgat ttccattagc agcagtacag aaagcggagt aagacgtgcg ggtttcagca
    11941 ccacacagtt gcccgccgcc agcgcggggg ccattttcca gctcgccatc agcagcggga
    12001 agttccacgg gataatctgc cccaccacgc ctaacggttc atggaaatga taggccacgg
    12061 tttcgctatc cacttcactg atcccacctt cctgcgcccg aatacacgag gcgaaatagc
    12121 gaaaatgatc aatcgccagc ggcacgtcgg cagcactggt ttcgcgaatg ggtttgccgt
    12181 tatcccaggt ttctgccgtc gctaaaagtt caaggttttg ttccattcga tcggcaatct
    12241 taaacagaat cgccgcgcga tcctgcaccg aggtgtgcgc ccatttatct ttcactttat
    12301 gcgcagcatc cagcgccaga tcgatgtctc gtttgcccga agacgccact tcgcacagta
    12361 gctgcccggt caccggcgtc aggttctggt aatactcgcc atcggcaggc gctacccatt
    12421 cgccaccgat aaagttgtcg tagcgggctt ttaatttgag ggggaaacca tactcgccgg
    12481 gcttaatctg tgctgaaggg ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat
    12541 aacaagggta gacgtgactg gcgaaatctt cgccagtcgg taacagcttt acgacggcta
    12601 tcacgaattt acgggcaaaa gctacagcgg gtagtgtgcg acgtacactg cttgctggtt
    12661 attaattctt ttctcttgcg cgaatccttc tttaacaatt gtccgtaaat cagctcgagc
    12721 cgtattatca gagacgccca agctcgcgct aatttcagca actgtgtact ctttccccgg
    12781 attggcgagc atcacattca ggagcgttac ctggcgcgga ttcaggcgtt taagcacacc
    12841 agagctaaac agcacactat cgagttttgc gcttcgttgt gtaatttcat cgatatgctg
    12901 ctgccagttt tgcagagcac gtttaatgac acccgcctga tattcaagga aataagtcag
    12961 atccatacca tctgattcag tgtactggta gctggcggca tatttgaccg gtgcagcatg
    13021 aagcaaacgg ctgatggaaa tatatttaaa gacatcgtag cccgatttca gcatatacca
    13081 gtaaaacagc gcccgactgg tgcgcccgtt gccatcccga aacggatgtt catgggccag
    13141 cataaaatgc agaatgcagg cacggaccaa cggatgaata tacccttcat ggctgtttaa
    13201 ccactggcaa actttttcca gccgttccgg taacaatgcc gcagcgggcg gttgatgcac
    13261 tatattgcca tcatagtcgg cgatcacaac atcatctgtt tcacgaaatt ctcccggacg
    13321 atatttcgcg tcattgattc cacccatacc aatggcatgt aactggcgaa taagcgcagg
    13381 tgtcagcggt tctgcgagta agtgcggaat ttccgccatc agccgggcat ttcccgcaat
    13441 catatgctcg tcttcagtgc gtggcgaccg ccctgtttcc agcatttttc tcgccaccag
    13501 cgttgtagta ttcgcacctt ccagctggga gctggtgatc ggctcttcca ggcgtaactg
    13561 gcttaactcg gctccggccc cgcgtaaccg ttcagtctga tctttcagcg ccagcgcggt
    13621 agcacgtttg tcgacaagct cgcacgcttc tgcaatcacc ggggtaatgt tgaatccggc
    13681 ctgttcaccc gcttcattgc aataattaat tcgttggatc gccgcgttgc gcgccagacg
    13741 ggtaagcgtc cacgccacat cgacgttttc tccttttttg atgcggtact gcaactcatc
    13801 aaaaggcaga taacgtcctt tttcatcggt aacccggtta aagcgcaggt gggccaggag
    13861 ttcctcaccc gataagtgtg ataaaggaat aagtccggga ggtgatttga tagccatgtg
    13921 cttatctcaa aaaagcgcaa tattgagata gtgtatagca ggaaagagaa actatctcaa
    13981 aaaaagcaag ttttgagata gttgaaaaag cggctgacaa ctcgccagcc gttgtgggaa
    14041 tgatgattac atcgctgcgc gataaatcgc cacaatttct tcgtgagttg cctggatcgg
    14101 gttagtaaag ccgcaggcat ctttcagggc attagtcgcc agaaccgcga aatcttcttc
    14161 tttcacgttc aggtcgcgta ggcctgccgg gatatccact ttcttcgcca gttcacggat
    14221 ggcgttaatg caggcttcag caccttccgc gtcgttttta cctgtcacgt tcacgcccat
    14281 tgcagcggca caatcacgca gacgtgcagc ggcgactttg ctgttgaata cctgaacatg
    14341 cggcagcaaa acggcgttac atacaccgtg tggcaggttg tagaaaccgc ccagctggtg
    14401 cgccatcgca tgaacataac ccagagaagc attattgaac gccataccgg cgaggaactg
    14461 ggcataagcc attgcttcac gcgctttcgc attactgcca tcttcaacgg ctaacggcag
    14521 gttttcggca atcatggtca cggctttcag tgcacaagcg tcagtgatcg gcgtggcggc
    14581 aatagaaaca tacgcttcga tagcgtgcgt taaggcatcc atacccgttg cggcggtcag
    14641 tgacttcggc ataccgatca tcagagagga gtcattgaca gaaagcagcg gagtgacatg
    14701 tttatccaca atcgccattt tgatgtgacg cgcttcgtca gtgatgatgc aaaaacgggt
    14761 catttcagag gccgtacccg ccgtggtgtt gatggcgatc atcggcagct gcggttttgc
    14821 agagcggtca acgccttcat aatcgcggat gtcaccgccg ttggctgcaa ccagcgcaat
    14881 accttttgcg cagtcgtgtg gagaaccacc gcctaaggaa atcacgctat cgcaattatt
    14941 ttctttaagt aatttcaaac ctgcagcgac gttttccgtg gtgggattag gttgggtgcc
    15001 atcataaata acgctaaaaa tattgcgttc ttccagtgct ttttgcacat cacccgccat
    15061 acccaatttc gttaacatat tgtcggtgac aattaaggta cgggtaaatc cataatctgc
    15121 catcatattc attgcatcat tcaatgaatc agcaccaata acattcacgg aaggaataaa
    15181 aaaggttgaa gctgccataa cacactcctg aaagtgtaaa aaagggcggg cagcttactg
    15241 agaattttca tt
//
LOCUS       DAJOTB010000114        15223 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJOTB010000114 DAJOTB010000000
VERSION     DAJOTB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.114"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(86..838)
                     /gene="yecC"
                     /locus_tag="OHN28_003496"
     CDS             complement(86..838)
                     /gene="yecC"
                     /locus_tag="OHN28_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCQ2298827.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(835..1503)
                     /gene="tcyL"
                     /locus_tag="OHN28_003497"
     CDS             complement(835..1503)
                     /gene="tcyL"
                     /locus_tag="OHN28_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCQ2298828.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1518..2504)
                     /gene="dcyD"
                     /locus_tag="OHN28_003498"
     CDS             complement(1518..2504)
                     /gene="dcyD"
                     /locus_tag="OHN28_003498"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCQ2298829.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEVAVNISSVVVASGSAGTHAGLAVGLEQLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2609..3409)
                     /gene="tcyJ"
                     /locus_tag="OHN28_003499"
     CDS             complement(2609..3409)
                     /gene="tcyJ"
                     /locus_tag="OHN28_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2298830.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3497..4048)
                     /gene="fliZ"
                     /locus_tag="OHN28_003500"
     CDS             complement(3497..4048)
                     /gene="fliZ"
                     /locus_tag="OHN28_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCQ2298831.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4094..4813)
                     /gene="fliA"
                     /locus_tag="OHN28_003501"
     CDS             complement(4094..4813)
                     /gene="fliA"
                     /locus_tag="OHN28_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCQ2298832.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4977..6026)
                     /locus_tag="OHN28_003502"
     CDS             complement(4977..6026)
                     /locus_tag="OHN28_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCQ2298833.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            6274..7689
                     /gene="fliD"
                     /locus_tag="OHN28_003503"
     CDS             6274..7689
                     /gene="fliD"
                     /locus_tag="OHN28_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCQ2298834.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7705..8115
                     /gene="fliS"
                     /locus_tag="OHN28_003504"
     CDS             7705..8115
                     /gene="fliS"
                     /locus_tag="OHN28_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCQ2298835.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8115..8480
                     /gene="fliT"
                     /locus_tag="OHN28_003505"
     CDS             8115..8480
                     /gene="fliT"
                     /locus_tag="OHN28_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCQ2298836.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKIKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8558..10045
                     /gene="amyA"
                     /locus_tag="OHN28_003506"
     CDS             8558..10045
                     /gene="amyA"
                     /locus_tag="OHN28_003506"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2298837.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10079..10492)
                     /locus_tag="OHN28_003507"
     CDS             complement(10079..10492)
                     /locus_tag="OHN28_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2298838.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            10679..11884
                     /gene="yedE"
                     /locus_tag="OHN28_003508"
     CDS             10679..11884
                     /gene="yedE"
                     /locus_tag="OHN28_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCQ2298839.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            11881..12114
                     /gene="yedF"
                     /locus_tag="OHN28_003509"
     CDS             11881..12114
                     /gene="yedF"
                     /locus_tag="OHN28_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCQ2298840.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12223..12720
                     /locus_tag="OHN28_003510"
     CDS             12223..12720
                     /locus_tag="OHN28_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCQ2298841.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(12602..12931)
                     /locus_tag="OHN28_003511"
     CDS             complement(12602..12931)
                     /locus_tag="OHN28_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2298842.1"
                     /translation="MKRLQAFKFRLRPGGQQERGMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            12954..13478
                     /gene="tnpA"
                     /locus_tag="OHN28_003512"
     CDS             12954..13478
                     /gene="tnpA"
                     /locus_tag="OHN28_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCQ2298843.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(13635..14432)
                     /locus_tag="OHN28_003513"
     CDS             complement(13635..14432)
                     /locus_tag="OHN28_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2298844.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLCYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(14442..14993)
                     /gene="ybcL"
                     /locus_tag="OHN28_003514"
     CDS             complement(14442..14993)
                     /gene="ybcL"
                     /locus_tag="OHN28_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCQ2298845.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
BASE COUNT         3823 a         3849 c         3666 g         3885 t
ORIGIN      
        1 ttatgctgat ttgttaagca gttttatcac gtttgaaatg gcgtcccgcc ccgactggtg
       61 aatcgtcggg gctgattttt tcttattatt gcagcagaaa cttctccagg aactggcggg
      121 tgcgaggctg ctgaggatca gcaaataacg cttttgctac cccctgctcg actatccgcc
      181 cctggtccat aaaaatcgcc cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca
      241 caatcaccat cgtgcgcttt tcctgcgcca gctgacgaat ggtgttcagg acttcgccca
      301 ccagctctgg atccagcgcc gacgttggct cgtcaaacaa aatcacttca ggacgcattg
      361 ccagagcacg cgcaatcgca acgcgctgct gttgaccgcc agacaaacga cgtggataac
      421 tggtttcttt accggccagc ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct
      481 cttctttcgg ttcgcctttg acgatcaccg gcccttcaat aatgttttcc agcaccgtac
      541 gatgcggaaa cagattaaag ttctggaaga caaacccgac gtgctgacgc aactggcgaa
      601 tcagcgattt ttgctgactt aatgaacgtg cagtatcaat agtgatatcg ccaacggtga
      661 tggttcccgc ttccggttgt tccagcagat ttatgctgcg tagcaacgtg gttttgccgg
      721 aaccactcgg accgataatt gccactactt cgcctggctt cacctcaagg tcgataccat
      781 gcagcaccgt ctgaccgtgg aattttttca ccaggttctt aacttcaatg gcactcattt
      841 gggttctctc tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg tcgataacac
      901 agtcgccatg atccagtaga tgagcgaagc cgccaggtac atggtgaaca cctccagcgt
      961 gcgcgaggta atcaactgcg cctgacggaa cagctccggc acctggattg tcgcggccag
     1021 cgacgtatct tttaccaggc tgataaagct gttcgacagc ggcggcaacg ccacccgcgc
     1081 cgcctgtggc aaaatcgcgc gacgcatggt ctgccatggc gtcataccaa tactggctgc
     1141 agcttcccac tgacctttat caatagaaga aatggcggcg cgcagcgttt cagcagcata
     1201 ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga atgggatcta attcgatacc
     1261 aaactgtggc agtccgtaat agatcataaa cagttgtgcg atgagcggcg taccacgaaa
     1321 aatagagata taaaaacgcg ccagccagcg cacgggccag attggcgaca ggcgcatcag
     1381 cgcgagaata aacccgagca gtaagccaaa aaacatgcct ccgatactga gttgcagcgt
     1441 atacccagcc cctttcagca ggaacggcaa ggaatcaata accagttgta tactttcttg
     1501 catgagcgtt cccggagcta aacgtgggga tgataggcga acagcgcagg cgcaccgccg
     1561 gtatgaataa acaaaatcgg cccttcatct ttgaagcgtt tctgactgat accgtcaatc
     1621 agccccgcca tcgcttttcc ggtatacaca ggatcaagca gaatgccttc cagcctcgcc
     1681 agcaatttca ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag
     1741 tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt
     1801 tggaggttaa ccactttcgg caattgatcg gcaactgaac gcgacacagt cacgccaatc
     1861 agttcgcttt caggcatcag ctgttccagc ccaacagcca gtccggcgtg agttccggca
     1921 ctgcccgatg cgaccaccac cgacgaaata ttaaccgcca cttcacactg ttgcgcgatt
     1981 tccagcgcac tctccacata acccagcgcg cccaaagcat tagaaccgcc aaccggaatg
     2041 acatacgggc gaaagccttg tgcttcgact cgcgtcgcca gctcttccag ttgggcattg
     2101 ggatcggtca gtgcgtcgca catttcaatc tgggtattga acagatccag caacaaacga
     2161 ttgccgttgg ttaaatagtt ttccgcggtt gtaccaatag gattttccag cagcgccacg
     2221 cagtgcagac cgagtttcgc agcgactgcg gcagtctggc gcacatggtt agactggatc
     2281 gccccggcag taatcagcgt atcggcacct tcacgcagag catctgccgc gagaaattcc
     2341 agcttacgta atttattgcc gcccattgcc atgggagtga cgtcatcccg tttgatgaaa
     2401 atttcccgtc caagataatc agaaaagcgc ggcagatatt cgagcggcgt tggcgcgcca
     2461 ataaactcca gccgtggaaa acgggttaaa ttatgcagtg gcataacagc ctccgatgtg
     2521 tgttgttgtg attttcttat tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg
     2581 aaagcgccct tttttgtcat tatgctgatt atttggtcac atcagcacca aaccattttt
     2641 cggaaagggc ttgcaaagtg ccatcttttt gcatttccgc aattgcatca ttcaccgctt
     2701 tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga ctcctgacga gagaatgctt
     2761 caccggttac tgccagcgta tcgttggttt tcttcaccag atccagcgcc gccagacgat
     2821 caacgaggat cgcatcgata cggcctacgc gcagatcctg atatttggtc gggtcatcat
     2881 cataggtacg cacatcgacg ccctgaacat tctgacgcag ccactcttca tagttggtgc
     2941 ccagaccgac acccactttt ttgcctttca gatcatcagc cgttttaatg gtgccttcgt
     3001 tgcctttttt caccagcgcc tgaataccag aaatggtgta cggggtagag aaatcgtatt
     3061 ttttcttgcg ctcgtcagaa atggtgacct ggttaatcac cacatcaatg cgtttagagt
     3121 ccagcgacgc cagcataccg tcccatttgg tcggttttag tgacgcctca acgccaagat
     3181 gttttgccag ttgttgggca aattccactt caaaaccggt taatttgccg tcatccccct
     3241 gaaaactgaa cggcggataa gttccttcca gccctaccag cagcgtgccg cgctctttga
     3301 ctttattaag cagaccttca tctgcaaaac ttttaacgct catacccgca accagcgcca
     3361 cggccatcac acccatcaat gcctgacgtc ccagatgtgc taatttcata ttcaccccga
     3421 atgttgttat gtctgtttgc agtgtagagc cattgtttgt aaacacaaaa acaactccgc
     3481 tacatcttat tcttatttaa tatatatcag aagcaggcag gctggaggat ttctgcacaa
     3541 gtccggtaca cgtttgacgc tgatagtgtt gatacttccg taacgcgatg cggtaattgt
     3601 tggtgctggt ggagggcagc cacggggcga ggatttcatc gaggaaaccg tcctgcagct
     3661 gatcgaggga aatattttgc tcgtgtagat gattccccaa acggcgcaga cgaacgacat
     3721 attcacgcac cgtaccaggg ctcatttcag tttgctcaaa aagaaattgc ttaaagccga
     3781 taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc accgcaaaat cggcacaatg
     3841 ccgcccatga ttgttgttcc acttgccagc cattttcatc aagcagcgtg tccaggcggg
     3901 aaatctcgat tttattcact attttgccgt cgcgaacgag ggtaatgcga tcgagtaatt
     3961 tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc tttaagatag cggcttaatg
     4021 gccgtctttt caggtgctgc accatcatta agaactcctg gtagtcaaaa ttaaagtgcg
     4081 gcatttactg acgttataac ttacccagtt tagtgcgtaa ccgtttaata gcctggctgt
     4141 gtaactgact gacccgcgat tccccgacct ccagcaccgc accaatctct ttgagattca
     4201 gctcttcctg ataatagagg gttaatacca gtttttcgcg ctccggcaac gtttcgatgg
     4261 cttccatcac ccgctggcgc agattattgt ccagcagttg ttgtagcggg ttttctcgct
     4321 gatgatcatc agtaaccagt tcgatgctat cgccgtgctc ttcgcgccac tcatcgtagg
     4381 agaagagctg gctgttattg gtgtcgagca acatttggcg ataatcggca atatcgatcc
     4441 ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc aagttcctgc tccagttgcc
     4501 ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct gcgcggcacc cagtcacggc
     4561 tgcgaagttc atccagcata gcgccacgga tacgctgcac tgcgtaagtt gtaaatgccg
     4621 ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa cccaatgccg cccgcctgta
     4681 gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg caggcgcaat gcttcgtgac
     4741 gcaccagcgg gacataacgc tgccacagcg agtgtttatc cattacacct tcagcggtat
     4801 agagtgaatt cacgataaac agccctgcgt tatatgagtt atcggcatga ttatccgttt
     4861 ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag gggttatttg ggggttaccg
     4921 gtaaattgcg ggcagaaaaa accccgccgt tggcggggtc aggcaggtta aggccattag
     4981 ccttgtaaca gagacagaac ttgctgcggt acctggttgg ctttagacag cacggagttg
     5041 cccgcctgct ggataatctg cgctttcgac atgttggaca cttcggtcgc atagtcggcg
     5101 tcctgaatac gggactgcgc ttcagacagg ttggtggtgg tgttgttcag gttggtgacc
     5161 gcagaatcca gacggttttg aacggcaccg agggaggagc ggaatttgtc gactttagcg
     5221 attgcggcat ccagtgcttt taacggatcg gctgtagcgg ctcccgccac ttccgctgca
     5281 ccaacagtta ccgccgccgc cgtaccggta atgactacag tggcttccat gtatttaccg
     5341 gttgcagaat caacgaccgc atatttagta ctgttagacg cggtgtcagt gtaagacagt
     5401 aaattgactt gtttaccccc agcatccttg acatccgcag cagcaattgc tgtaccatca
     5461 gcagccgtca gtttcgccgc gctgaaatcc gccatgccgg acacggtaaa tcctttcaga
     5521 ttcaaagtgg tactgtcgat ttgttgcaag ccaatgctga tcgtctggtt atcattggca
     5581 ccaatctgaa tcttcatcga atcgtttttc gacagcacgt tcacaccgtt gaactgggtc
     5641 tgaccagata cacggtcaat ttcatcaaga cgggatttga tttcgtcctg aatagaagac
     5701 aggtcggaat cagagttcgt gccggtagag gcctgaacgg tcagttcacg aatacgctgc
     5761 aagttgttgt taatctctga cagcgcgcct tcagccgtct gcgccagaga aataccgtcg
     5821 ttggcgttac gtgcggcctg agtcagacct ttgatgttag aagtaaagcg gttagcaatc
     5881 gcctggcccg cggcgtcatc tttagcgctg ttaatacgca gaccagaaga gaggcgctcg
     5941 atagaagtcg acagcgcaga ctggtttttg ttgatgttgt tctgagtgat cagcgagagg
     6001 ctgttggtat taatgacttg tgccataatt cattttcctg ttttcaagtc tacggtttaa
     6061 gtattgggct tccacccttc ggcttcgtcg ccgtcaaccc tgttatcgtc tggtcactca
     6121 caacctttag aatttttttc tagcggtcga aatactcccc ttcctcgttg ctgttcgtgc
     6181 cattaaggca aaaaataaat cgtaaacttt accccgctta cgccgataac cccggtattc
     6241 gttctataca cgtcgcatga ttaaggaaac tatatggcta gtatttcaac gcttggggtt
     6301 gggtcggggc tggacttaag cagcatcctt gacagtctgg aggcggcaga aaaatctacc
     6361 ctgacgccga tatcaaaaca gcaaagctct tacaccgcga aactgagcgc ctacgggacg
     6421 ctgaaaagtg cactagagag tttccagaca gccaacacag cactgaataa agccgattta
     6481 tttaccgcca cctcaaccac cagcagttca tcggctttca gcgccaccac cacgggcagc
     6541 gcgattgctg ggaaatacac cattagcgtt agccaactgg cgcaggcaca gacgctgaca
     6601 acaaaaaata gtcaaaaaga cagtaaagcc gccatcgcca ccagcgatag cgtactgacc
     6661 atccaacaag gcggcggtaa agatccggtc acgattgata tcagcgcggg taactcgtca
     6721 ctttccggca ttcgcgatgc cattaacaac gccaaagctg gtgtcagcgc cagtatcatc
     6781 aatgtgggaa acggcgaata tcgtctgtcc atcaccgcta acgatacggg cagcaataac
     6841 gctatgaaac ttagcgtcag cggcgacagc gccctggaaa gttttatggg ttacaacgga
     6901 acgccgggtg atagcagcaa tggcatgatt gaaagcgtca ccgcgcaaaa cgccaaactg
     6961 acagtcaata acgttgagat tgaaaacagc agtaacacta tcagcgactc actggaagat
     7021 atcaccctca acctgaatga cgtcaccacc ggtaatcaga cactgaccat cagtaaagac
     7081 acatccaaag ctgaaaatgc ggttaaagcg tgggtggatg cctataacac attgcaggat
     7141 acattcagca gcttaaccaa atacaccgct gtcgatgccg gagctgaaag tcaggactcc
     7201 agtaatggcg cattactcgg tgactccaca ctgcgtacca tccagactca gcttaaaacc
     7261 ttgctcgcca atactcacag cagttcaaac tataaaacgc tggcacagat tggcattacc
     7321 agcgatgcca gtaccggcaa gctggaaatc gcgaccgaca aactgcaaac ggcgctaaaa
     7381 aatgatgccg ctggtatcgg tgaaatgttt ataggtgacg gcaaaagtac tggggtaacc
     7441 accggcatta gtagcaacct gaccagttgg ctctcttcca ccgggataat ccaggcagca
     7501 aaagatggcg tcagcaaaac gctgaataat ttgaccgacc agtacaacgc agccagtgaa
     7561 cgtatcgata ccttaatgac gcgctataaa gcccagttca cccaattaga tgtgttaatg
     7621 aactcgctga actccactag tagctatctg acacagcagt tcgacacctc aaatagcaac
     7681 tcgaaataat cttcaggaga gactatgtac gcggcaaaag gcacccaggc ctatgcacaa
     7741 attggcgtcg aaagcgccgt aatgagcgcc agccaacagc agttggtcac catgctattt
     7801 gatggagtgt tgagcgcact ggttagagcg cgcctgttta tgcaggacaa caatcagcaa
     7861 ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt
     7921 cttgatgaag agagcaaaga cgaactaacc caaaacttga ttgctcttta tagctatatg
     7981 gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct ccgcagtcga agaagtggaa
     8041 gcattaatgc gcaatattgc cgatgcctgg aaagagtctt tactctcccc ttctttgatt
     8101 caggacccag tctgatgaac aatgcaccgc atttgtattt cgcctggcaa caactcgtcg
     8161 aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa ctcatcgcca
     8221 gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca cctgactgaa gaggttgccc
     8281 cgtccactac gatgcaggag cagctccgcc cgatgctgcg cctgattctc gacaacgaaa
     8341 gcaagataaa gcagttatta cagattcgga tggatgaact ggcgaaactg gtcggtcagt
     8401 catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc
     8461 cgcaggataa cctcttttga atcttaatga gccgatggct cgcgaataat ccgattacgg
     8521 ctacgcttct aatattcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca
     8581 atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc
     8641 agacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc
     8701 atcgggcggc tattcggtcg gctacgactc ctatgattta tttgatctag gtgagtttga
     8761 tcagaaaggc agcatcccta ctaaatatgg tgataaagca caactgctgg ccgccattga
     8821 cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg gtagtcaacc acaaaatggg
     8881 cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc gtacgcaaat
     8941 tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg ctcgtgccgg
     9001 gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaaa
     9061 ccctgatgaa gatggcattt ttaaaattgt taacgactat accggagaag gctggaacga
     9121 tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggggagaata tcgattttcg
     9181 caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacgca
     9241 atgcgacggc tttcgtcttg atgcggtcaa acatattccg gcatggtttt ataaagagtg
     9301 gatcgaacac gtacaggaag ttgcgccaaa gccgttgttt attgtggctg aatactggtc
     9361 gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt
     9421 tgatgcgccg ctgcagatga aattccatga agcatcgcgc atgggtcgcg actacgacat
     9481 gacgcagatc ttcactggga cgctggtgga agccgatcct tttcacgccg taaccctcgt
     9541 tgccaaccac gacacccaac cgttgcaagc cctcgaagcc cccgtcgaac cgtggtttaa
     9601 accgctggcg tatgccttaa ttttgttgcg ggaaaatggc gttccttcgg tgttctatcc
     9661 ggacctctac ggtgcacatt acgaagatgt cggtggtgac gggcaaacct atccgataga
     9721 tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg
     9781 tgtacagacg ttatttttcg accatccgaa ctgcattgcc tttagccgca gtggcaccga
     9841 cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat gatggcgaaa aaaccattca
     9901 tctgggagag aattacggca ataaaacctg gcgcgatttt ctggggaacc ggcaagagag
     9961 cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt
    10021 gtgggttatc gaagaggtga tttaaatcca tccccggcgg caagccgggg agatttcatt
    10081 acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct gccgtcgggc
    10141 gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca cgctcaacct
    10201 cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat gtcaacttac
    10261 ccggcacggc aatcacccgc tgccactgac ggcaatccag ggtatcgccc tctttagtga
    10321 caatgaggct gcctatcgct tccggactga ccaacgcacg ttgcggccct ttggtctgcc
    10381 agtaacccgt cagccagtcc ggcgcggggg ttttcacgac attgttatag ttttcgactt
    10441 cggcacagcc cgccagcaac atgagcgcac ctgcaattgc gagttttttc atcattcgtc
    10501 ctgcatgaga agaaaagagg attgtggcat taaagccctg atgtcgccag cctttccagg
    10561 ttcagagaga aatttgatct aaccattatt tttgtgaatt attaccccga aaataaattt
    10621 gcatggtgta tgattcgcga ctcttgtttc aaaccttctg agttcagagg ctacattcat
    10681 gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcgccca tccccgcggt
    10741 catcgcggcg ggtattctct ccacttacta ttttggcatc actggcacct tttgggcagt
    10801 cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ttgttcggcg tccatgctga
    10861 agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc gcatcgacgg
    10921 aatgatgatc ctcggtatgt ttggcggctg ttttgccgca gcgctgtggg ccaacaatgt
    10981 caaactgcgc atgccgcgca gccgtatccg cattatgcag gccatcattg gcggtattat
    11041 cgccggtttt ggcgcacgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggaat
    11101 tccacagttc tcgctgcacg cctggttctt tgccatcgcc actgccattg gttcatggtt
    11161 tggcgcgcgc tttacccttc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc
    11221 tgctgcctca ccgttgacgc aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat
    11281 gctggtcttt ttcggcatgc tgggctgggc gctgctgacg gcgatgaacc agcccaaact
    11341 ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg
    11401 ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat
    11461 cattatcggt atggcggtaa gtgccatcgg gatcttcagt tacgtacagt taggcgttga
    11521 acccaaaatc atgtgggcgg gaccaaacgc ggtaattggt ggtttactgt ttggttttgg
    11581 catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt
    11641 gcactactgg tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtactactg
    11701 ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacctt
    11761 tggcccgatg ggcggcctgc tggtgacata tttgctgttg tttgctgctc taatgttgat
    11821 tattggctgg gaaaaacgct tcttccgccg tgcggcaccg cagactgcta aggagatcgc
    11881 atgaaaaata tcgttcctga ttaccgtctt gatatggtgg gtgaaccctg cccttatccg
    11941 gcagtcgcaa cccttgaggc gatgccgcag ttaaaaaaag gggagatcct ggaagtggtg
    12001 agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctatacg
    12061 gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc
    12121 attcccgctg tggcccacag cgggaacatc gttacctctc tatcacatag attttacccg
    12181 ccctctctcc tattcttttc agtgtgctct caggaggttt ctatgtgtgg acgctttgcc
    12241 caatcccaaa cgcgtgaaga ttacctcgcg tttctcgcgg aagatattga acgcgatatt
    12301 ccctacgatc ccgaacctat tggcagatac aacgtcgcgc cgggaaccaa agtccttctg
    12361 ctcagtgaac gtgatgaaca ccttcatctg gatccggttt tctggggata tgctcccgga
    12421 tggtgggata aaccgccgct gattaacgcc cgcgtagaaa ctgccgcaac cagtcacatg
    12481 tttaaaccgc tctggcaaca tggtcgggca atctgttttg ccgatggctg gtttgagtgg
    12541 aaaaaagaag gcgacaaaaa gcagccttat tttatctatc gtgctgatgg acaagctgtt
    12601 tttatagccg cgataggtag cacaccgttt gagcgtggtg acgaagccgg aagaagtttt
    12661 tgtaagcccg ctcagggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa
    12721 gccattgcgt ttcagtggca tttttccact caaccaacca ggaagccatt ttcccgtaag
    12781 ggatgtattt attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa
    12841 cgaaacgaca tgcgcctgcg aagcgcctca ttccacgctc ctgttgacca cctggtctta
    12901 accggaattt gaatgcctgt agtcgtttca tgggccaaat catactttgg cctatgaaaa
    12961 aagaaaccga tatccgacgc ggaaggcatt gtgttttcct gaagcatgtt cacctggtct
    13021 tcgttactaa ataccgacgt cagatttttg accatgacgc gacagaaaaa ctacgcactt
    13081 acttttcaaa tgtatgtgct gattttgaag ctgaacgggt tgaaatggat ggcggaccag
    13141 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
    13201 gcctcaaagg cgtatcgagt aggttactgc gacgagatcg accagatatt gcagtcaggt
    13261 attacgacaa aggcgttttg tggagtcctg gctattttgt cagtagctgc atcttaacgt
    13321 atcaactaga tttaataaca acgatatccg tacctaaccg gatatctaat gtcataagca
    13381 tccctttaat tatcccctca acctcttgta actttcccac tcttgagctc cacatagtcc
    13441 aaacgccgga tgcaaaaaaa tcacacgtgc atttctgaaa ttgtgatgga ggaagatagt
    13501 atcaattata tgtgcttaga aacgtccccg attagcgcag aagtcaccgg attgttcaga
    13561 ctccaatagg ttgagtatgc cgtatttatt ttccaaaata aaaatcgatg ctgttgctcc
    13621 ttcataaaat acttttactc tctggacgct tttcttagct ctcttggtgt tatcttaaaa
    13681 cgattatgga acctttcagt aaaacgagaa ggacacttgt aaccattttc cctggcaatc
    13741 tcgctgatgg gttttactgt cgtttgtata gcagacaacg cattatttaa cctcacgtcg
    13801 tccaatatac tttgaaaact tactccctcg cttgccagac ggcgatgcaa tgtggaaaca
    13861 gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttccagg ttcggatagc
    13921 attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag taaacaatta
    13981 acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg aatttgcgtt
    14041 gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa ctcggggttc
    14101 tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata aaatttttga
    14161 aacttttcaa ttagatcaat gggataacaa agcatttctg caagataaag acctgcttct
    14221 ggataattcg caatataaaa ttcatatcca cagggaaata atattatctg attattatca
    14281 acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat acgacataaa
    14341 gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca
    14401 atctttaggt tagtcttatc ttttgccagc atactcttac cctactttat ctcataaact
    14461 ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg
    14521 ctggaattag aatccacagg aattttatca gtttttagag cccatacttt aaactggtaa
    14581 tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata accaaaatca
    14641 tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagcg
    14701 ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca tccactgcct
    14761 gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc aggagcacca
    14821 gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga aaatacatga
    14881 gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc
    14941 tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc
    15001 tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta tgcataaagg
    15061 cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt agccataata
    15121 tgcacaaatc agattcctgt tatctcaagg ataattattt tttaatgcgg tgcacttcgt
    15181 gacaacaagt taatgactaa cacaccggca caaatcaaca tca
//
LOCUS       DAJOTB010000115        15131 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJOTB010000115 DAJOTB010000000
VERSION     DAJOTB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.115"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(256..1113)
                     /gene="repA"
                     /locus_tag="OHN28_003515"
     CDS             complement(256..1113)
                     /gene="repA"
                     /locus_tag="OHN28_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCQ2298846.1"
                     /translation="MADLLQKYYSQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(1106..1588)
                     /locus_tag="OHN28_003516"
     CDS             complement(1106..1588)
                     /locus_tag="OHN28_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298847.1"
                     /translation="MANHETHSVIAGGIESDLHIDSSKYPHPFCSLLQKRAHFDSFKH
                     LIFIRGLLAIAHGKRKNKISASMVSFYVSLVQVMCAHYIPDTNKVNLSVKELSVCSGG
                     SYTRVWRALKTLDNDLHLIAFDGGTIWFRPDMFETLRVGPDELVTARRRGNSVGGGHG
                     "
     gene            complement(1581..1655)
                     /gene="tap"
                     /locus_tag="OHN28_003517"
     CDS             complement(1581..1655)
                     /gene="tap"
                     /locus_tag="OHN28_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCQ2298848.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCG"
     gene            complement(1887..2144)
                     /gene="repA"
                     /locus_tag="OHN28_003518"
     CDS             complement(1887..2144)
                     /gene="repA"
                     /locus_tag="OHN28_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCQ2298849.1"
                     /translation="MSQTENAVTSSSGTKRTYRKGNPVPARERQRASLARRSNTHKAF
                     HAVIQARLKDRLSELADEEGITQAQMLEKLIESELKRRATS"
     gene            complement(2428..2634)
                     /locus_tag="OHN28_003519"
     CDS             complement(2428..2634)
                     /locus_tag="OHN28_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2298850.1"
                     /translation="MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
     gene            complement(2938..3012)
                     /locus_tag="OHN28_003520"
                     /pseudo
     CDS             complement(2938..3012)
                     /locus_tag="OHN28_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(3258..3470)
                     /locus_tag="OHN28_003521"
     CDS             complement(3258..3470)
                     /locus_tag="OHN28_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCQ2298851.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(3606..4166)
                     /locus_tag="OHN28_003522"
     CDS             complement(3606..4166)
                     /locus_tag="OHN28_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCQ2298852.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAGRLEKIRRQNRTKAELQAVLDGK"
     gene            complement(4269..5129)
                     /locus_tag="OHN28_003523"
     CDS             complement(4269..5129)
                     /locus_tag="OHN28_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2298853.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYKGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(5188..5934)
                     /gene="traX"
                     /locus_tag="OHN28_003524"
     CDS             complement(5188..5934)
                     /gene="traX"
                     /locus_tag="OHN28_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCQ2298854.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            complement(5954..11224)
                     /gene="traI"
                     /locus_tag="OHN28_003525"
     CDS             complement(5954..11224)
                     /gene="traI"
                     /locus_tag="OHN28_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCQ2298855.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHNTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYTLIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQEAMQKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAILRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNNVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDSKTEQAAREIAGQERDRSAISEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            11306..11533
                     /gene="vapB"
                     /locus_tag="OHN28_003526"
     CDS             11306..11533
                     /gene="vapB"
                     /locus_tag="OHN28_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCQ2298856.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            11533..11931
                     /gene="vapC"
                     /locus_tag="OHN28_003527"
     CDS             11533..11931
                     /gene="vapC"
                     /locus_tag="OHN28_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCQ2298857.1"
                     /translation="MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(11940..14093)
                     /gene="traD"
                     /locus_tag="OHN28_003528"
     CDS             complement(11940..14093)
                     /gene="traD"
                     /locus_tag="OHN28_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCQ2298858.1"
                     /translation="MSFNAKNMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGREMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEVNINVHRERGENVEPGDDF"
     gene            complement(14346..15077)
                     /locus_tag="OHN28_003529"
     CDS             complement(14346..15077)
                     /locus_tag="OHN28_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HCQ2298859.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQFKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT         3348 a         4468 c         3652 g         3663 t
ORIGIN      
        1 ctgtaaggct ggcggatggc gctttatgag atgaacggtt gtaaccttat ggggaagtcc
       61 attgcagtta aatgtggata agcaaaattc cccgtctgtg aggcgtgttt tgtatcaaaa
      121 acaggggggc cagatgcacc agaaggtgga tgatgaggtt gttttttgta tgaggtgctg
      181 cttttttgtt cgctggcagg cttcaggcgt gcgaatgcct ccggtgcggg ccggattatt
      241 cagaagaggt cactttcagg gggaagctgt ggccagccgg ctgtaattac ggttacgtga
      301 cagaatcatg cgctctttca cacgacgctc cacttcgcgt tttaccgcct cacgattggc
      361 agtgaagcgc ccttccgaga tttcacgcgt cagctgccgt ttcaccaggg tgacgatatc
      421 ctgacgttcc ctgttcgcat cacgacgcgc acgggcacgc tttattccac gggacttaag
      481 ctctgtctgg taactgcgga aacgctcacg cacaaaacgc caggctttcg ctatcagttc
      541 atccataccc agggtatcca gcccctgctt tttgcgctgt ctgttttccc attcaacacg
      601 actgcggcgc gcagcaacca ctgcatcctc agacacatca agggcggcaa ataacgccgg
      661 tgtgaacgtg atatcggtcg gaatgtagca cccgataagc ggatcatatt ccgtctggta
      721 ggtaatcagt cccagctctg caaggaacgt caaggctcgg gaggcccggg tgatggagag
      781 ttttccggca gcagactccg tcgccagtcc gcactcaatg gccagcgtag tgatggagca
      841 ctggacgcgg ttggccagcg ggtcatagtg aaaacacagc ccctgcagca gcgcatcaat
      901 agcccggcga cgcagtaccg gtggcatgcg ccgacgcagt cccttcgaac gggcatgtgc
      961 cacatgaatg gcgaaatcaa aacgggaggt gaagcccacc gccttttcca tcagtttttc
     1021 gcagaacttc agcgttccgg caccttcacg gggtgtgaac accggattcg gatttttaac
     1081 ctgtgaatag tatttttgaa ggagatcagc catgtccccc accaacacta ttccccctcc
     1141 tacgggcggt aactagctcg tctgggccga cacgtaaagt ttcgaacata tctggtctga
     1201 accagatggt tcctccatca aaagcgatga gatgaagatc attatcaaga gttttgaggg
     1261 ctctccaaac acgagtgtat gaacctccac tacaaactga aagctcttta acagataagt
     1321 taactttgtt cgtatcaggg atgtagtgtg cgcacattac ttgtacgagg gaaacataaa
     1381 aactaaccat cgacgcgctg attttatttt tacgtttgcc atgcgctatt gcaagcaaac
     1441 ctctgatgaa gatcagatgc ttgaaggagt caaaatgagc ccttttctgg agaaggctgc
     1501 aaaacgggtg aggatacttg ctggaatcaa tgtgaagatc tgattcaatc ccacccgcta
     1561 ttactgaatg tgtttcatga ttagccacac catcctgcac ttacaatgca cagaaggagt
     1621 gagcacagaa agaagtcttg aacttttccg ggcatataac tatactcccc gcatagctga
     1681 attgttggct atgcggtttt atggggcccc ggtaatcttt ctagtcgcca aacttgaaga
     1741 agattatcgg ggttttcgct tttctggctc ctgtaaatcc acatcagaac cagttccctg
     1801 ccaccttacg gcgtggccag ccacaaaatt ccttaaacga tcagcaatct accactcact
     1861 cctgagataa gcaagaatgt gaatatttac gaagtcgctc tgcgtttcag ctctgattca
     1921 atcagttttt caagcatctg cgcctgagta ataccttcct catccgccag ttcactcagc
     1981 ctgtctttta accgggcctg aataaccgca tgaaaagcct tatgagtgtt gcttctgcga
     2041 gccagagaag ctctttgcct ctctctggcc ggaacagggt tacctttccg gtatgtacgc
     2101 ttggtgcctg atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaattc
     2161 tcaaacaggg atcgtttcgc agagaatact acagtttttt gaaatcagcg acttgaaagt
     2221 tgtgacgatg atctgtgttg gcgcacaaat catgtctgat tacgaccgtt taatgtgatt
     2281 ttgataatga aatgactgaa aagctggaaa tgccttagcc agggtgccgg ccccgggcgg
     2341 gaccggctgt ttctggtata tagggtgtct gcccctgaaa tacctcacac ttccggaaag
     2401 cggggacttc cgtccccgcc cggcagctta cttccgtgct tcgtaggcca gagttacctg
     2461 caccactgta tttcctctgt gaatattcag ctcacataac cgttccctga atatcagtga
     2521 aaaacacaac accgtggcgc acacggcgag caacccgata agggcatatt tcgtcataaa
     2581 ctttgacctc tcagcaaaga agaggctaca atcagtagtg ttaaggtact tcatgcagac
     2641 ctcacgagtt aatggattaa cgagtggggt cttcgcattt ctggtacctg cgtcttgcct
     2701 gaaccagaaa ggctcaaaca cccgccgcga taataccaca ttccctgttg ctgactatta
     2761 cccccatttg cctgtttttg ttacaaaaac gccgggagcg ggggaaaact tactttttcc
     2821 cgtttccggg ggggacaact gagcattgcg aacgcgtcag ctacgatgtt ccggtgattg
     2881 ctgatcagcc cctgttgtat tcataaacta attaacattc attctgtctg gtgcataaat
     2941 ctgcccgtga acatctgcag acaataccgg ccatgaaaag ataaataata ccagtggaat
     3001 atatttcctc atgttattct ccatatcaga taaatggcac ctgtatttca gcccccctga
     3061 atatcgcagc acacattgaa taagacctta ttttgtgaaa ttaatcacaa aatataaaat
     3121 cgcgacttaa tacggaaaaa actctgtgct actcagaact ggccggtcat actgcccgac
     3181 cggatattaa aaaaagcaaa tctcataaac tcctttcagc cttctgactc ctggagtaat
     3241 gaaggctgct tttttcgtta cacccttccc cggcagcgcc ggtggcagtg ttcagccctt
     3301 ttccgggcaa actgttgcca gtctgtccgg ggagctatgc aggcagcccg tcgccatgct
     3361 tctgccgctt ccatccagcg tccactgacc tccagaataa cggcccgccc gccggcacgc
     3421 tggtaacgcc agacctgacc gttccgtgaa ctgtttctga atccattcat ttttctgctc
     3481 ctccctttta gtcgtcagta ttattgtggt aaataattca gtggactggc ttcctttcgg
     3541 gtacggaagt tatttccttt taagccttat tgtttcagcc tccccggttt ccggggaggc
     3601 tttttttatt tcccatcaag cacggcctga agttctgcct ttgtccggtt ctggcggcga
     3661 attttctcca aacgccctgc cgcatacgct tcctcctcct gagaaatatg ctccgtcaca
     3721 tacccttccg tgtcataccg gcaggcaccg gctttcatgg cacacagata gctttctgaa
     3781 cgggtgatgg ccttcagcgc ccggcgcagt tttttatgcg agagcgggat attccgctgc
     3841 gccacgtctt ccagtaacac gtcccggata ccgcaggcca gaagccgggg cgtgttaccg
     3901 tcaaacaatc ccggccacca aggtttcaga gtgttcaccg cctcatccag cgagggcagc
     3961 gtcagataaa tggacagcgc ctgtctggcc tgtgctttct tcgccgccag ctctgcttcc
     4021 cgggcagcct tctccgccag tttctgcttt ttcaccttcc attttggtgg cgtggtgaca
     4081 ttgatgatgg ttttccggct ccgggtcggt gtttcccctt ccgttttccg tttcagtgtc
     4141 agtaccggtc gtttctgctc tgtcatgatt gccctgacct ttgtgaaaat atgtcctcca
     4201 ggtcgttgaa actactcact atgatattaa gctgacctca acgacctctg acatattaat
     4261 accgacaatt aaagatgttt tttaaaccat tgcgtctgta ctgccgccac acgttcaaaa
     4321 aacgcgcctt tataaatatc ataatggcat gcgtcagctt cctcgtataa ttccttagta
     4381 ccagaagcaa cagcatcata taaggctctt ccctgttctg gtggattaac actatcctgg
     4441 ccggcaataa caaccaaaac tgggcactgt actttggctg cgctctctgc cggtttatat
     4501 tgcaacgtct ccataaccgt aaggaaaggt atttttatat ccatttcagg atactgacct
     4561 tttaccttct cgaagaatac ctttgattca ttatcactca gtacccgggt aacccccaca
     4621 aacatctctt tcccggtatt tttcttcttt tctgccattt tattcagcgt ggacagaaaa
     4681 gaagctttct ccagttcatt catttctccg gtaaccagaa cctcaccgtc agcaaaagcc
     4741 agctggctga caatactttt cactctttta tcctgcgcag ctgcattgaa tacatgacac
     4801 ccacccagcg atgttcccca taatcctata cgctgattat caatacatac ctgcttttct
     4861 gcccaattga taactgaaat aatatcttcg gtctgcattg ccggaaccag acgacctcgt
     4921 tccccttcac tctcaccgaa tccacgataa tcaaaagtaa tcgttgcaaa cccggcttct
     4981 gtaaacgcat tcgcaaagga aggcagcaga acattccgga taccgcaaaa tccatggcat
     5041 aagagaatca aaggatgttt tatatttcct tccgggacac gaaatgtcag ggcaatacct
     5101 tcagggagtt tgtgatccgt aattttcata aatatcctgt gttatgttgt ttattaataa
     5161 gcagaatcag cgccagcatc ataccagtca cacagccaga acgcccagca cagtaagatg
     5221 acaggcatag aacgtcggga aaaaatcccc gtaccagaaa cggggtagtg atttccctgt
     5281 acaggacacc agaccaacag tcagcaccgt catcacaagg ccggccaccg ccgctgcatc
     5341 actggtggca aggttaagcg ccggaataac agccagcagg caggcgacca gcgccagacg
     5401 ttccattctg tcttccgccc ggtacagcct gtggctgact gccagcatca gcagcccggc
     5461 aatgccgtaa ctggtgccgg agaaaggcaa ccacattgcc agaagcaaca tggtctctgc
     5521 ggaacgccac caggtgcgcg tttcacacca tgtcagcacc tgtgccgcaa ctgcaaaggc
     5581 aaacaggata ttcccctcat accacggaaa gcctgccaga taatacgcga actgggcaat
     5641 aattccccat ccccacagcc ggttaatggc cgactgcctg atatgcgcat ggcgggacag
     5701 attcagcccc cacaccaggg caaacaacgg aaaggctcca cgcccggcca gaaacatcca
     5761 cgtctggtcc agatgcagaa tgcggtttgc gtggtccagt accatcagca ccagtgccac
     5821 cgttttgatg atatcccgct gtccgggtga ccagaccagc aacgactgca accaggtatc
     5881 ggtccgggca gccagtgaat cgttacgtgt cgtgttcgtg ttatccgttg tcatcagtgt
     5941 ttgtcttcct gtatcagtct ccgcccaggg ttttctcttt ctgcaggtca cgaaccatat
     6001 cccgctccat ctgctgaagt cgttcctgca gcagattttc ccgggcaacc tcccggaccg
     6061 cttcccgctc ccgttgtgat tcacgcagca cactctccgg cagcgcggct tcccgttcag
     6121 atatggcaga ccggtcacgc tcctgcccgg caatctccct ggcagcctgc tctgttttgc
     6181 tgtccggcag gtcaggtttg ttctctgcca ttttacggac aatttcatct gcgcggcgtt
     6241 ccgtttcacg gcggatggcc tcttcagcct gccgctgtgc cagtacctct gccgtgaccg
     6301 gctcgccatt tccggctccc ggctggacgt tgttgtccgg gatatccccc cacacgcggc
     6361 caccggtgat ggcaccggga ttccacggac gaccttcacc ggcgattctc accaccacac
     6421 cgctgtcggg gttatcacgg gcaatccgga caccatcctg catattatca gccagcaggc
     6481 tctcgccgtt acggctgccc tgcagggcca cgaactgcgc atccccgctg cctttcacac
     6541 ggccttcacc actgaatccc cgcagcccgt ttccgtcatc cgtggtcagc gggttcagcc
     6601 agataccggc agacttgccg ttacggtcaa acgccggcag tgccacatac ggctgtggat
     6661 atttacgtcc cggagcaata aaccgtgcag gactgtctcc cccggcaagc cccgcctgac
     6721 gaagaatggc acggcctgcc gccacgtccc gcagctcccg cgccgtactg aacagccgct
     6781 ctgcattcat gacctcccgg tccggtttcg gctcaaacac atcgtgggca gttcctttct
     6841 gtacggcatt gttaatggca tccgtccagc cctgacggtt atcggtgtac acctgcacat
     6901 gctgcttcat acgcgacagg gccacgtagg ctgactcaaa gccggccatc tgtttccggt
     6961 taccttccgt gccttcaagc gcaatggcaa aggtttcact tgccccctgt gcaccgtggg
     7021 cagtgatggc ataggccagg tcaatatgtt gctctgcccg ctcctggccg gggcgaatca
     7081 cccgggtctg ctgtccgtcc gacagcgtga cactgtcacc ggaaaccgct gtcaccgtcc
     7141 agacgctgtt ggccacataa ccacgctccc ggtcactctt cgtgaagcgc atccggtcac
     7201 cggtccccac ccggatggtg tccggagtgt acagtgtgac gccttcagcc accgcctccc
     7261 ggggcgaaat cagccgcgtg ttaccttccg catcctgcag ggttatcagc ccgtcatcct
     7321 tactgatacc ggcaatccgg tgatacacat tatccaccag ggcaagtgcg tcccgatgag
     7381 tctcccaggt ggagagacga cgcaactccc cgtcgcgtat attcgctgtg ttcaggacag
     7441 gcaccatgac ctgctctttt cccagctcac cggccttttc ccgtgcatca tgaatcatgc
     7501 tgttcagcac gcgcctgtct tcattcaggt gcgtgacaat cagcgtctgc tcccgtgctt
     7561 ccggtgttct gccggtatag tcgcggacaa tggcttcata cagtgtcatg ggaacatccg
     7621 gaaaagcctc gcctttctgc atcgcctcct gctgcgcttc tgccagtttc gcttcctggc
     7681 tgtgactgaa ctccgtcacg gagtgctccg gtgcccatgc gccctcctga cgtggcacct
     7741 gagacggttt cacactctca agcccggaca gtgccctttc cacatcccgg ttaatcaggc
     7801 tgtataccgc ctcccgcagt tccggcgtct gacgcacaat ctccttcatg atggccacat
     7861 cggcagcact gcgcgtctgc tggagacgga aaggctgacc gggcgcgatg gcctgcagct
     7921 ggtccgtgtc accgctggcc acggcacgac caccaccggc cgcaatcagg gtgtatgccc
     7981 gtgccatgtc ggtattgccc accattgagc tctcatcgag caggaacagc gtgttgctga
     8041 aatccggcgt ttctccgctg cgctgctgca gctgcgtgtc atgcagaaag gacgccagtg
     8101 tctgtgcatc cacgccggcg ctgcgcatct ccccgaccgc acggtgcgtg gggcccagcc
     8161 caacgacacg gggacgctca ctctccggca gcatgttcac ggctgacatc accgcccgga
     8221 actgtgtggt cttacccaca ccggcatagc cctgcaccac cgtgaaacgg tcgggcgttt
     8281 ccagtatcat gcgggtggcg gcgcgctgtc ctgatgtcag tttctccatg agttcgccag
     8341 gtactctctc catcagcggc gtgaccgcct ccttaccttc gagaatatgg cgaagaatgc
     8401 ttttttcagc ctcatacgac gcacgggaaa ccagcaggtc tgtgccataa ccttttgcca
     8461 catccacatg cagtaaatcg ccccgtttta tctgcgcatt gatttcccgc cccaggtcag
     8521 caaaactggt tccttctgca gcaaacgact ttgcctctgt cagcaggtcc acatggctga
     8581 aggccaggtt tttactttcc accaccggaa gggcaagatg aatggcctgc tgtgccggcg
     8641 tgttaagtgc ggacttctga tgactgatag ccgtttccag cgatgtctca ccggcccgcg
     8701 cctttatctg ctcagaaacc accgtaaagg acggatggcg ggcaagtttt tccgcagtac
     8761 gggtttcatc cagtgaggaa tacagccgga catcacgacc actgcgggcc agaccgttca
     8821 gggtggcatt gtccattgcc atctgtgtga cggaggcaaa aaccgtcgca ctgtcactga
     8881 cggaatgccc gggcgtttcc acccagccat tctccagctt cagtgccgtg aatggtgagt
     8941 cgctcacagg cagggaggcc ggttcagccc gccccggcac aacaaccgtc atcgcatctt
     9001 cactgacgga tgtcacctgc aggcggtcac cgccggagac gcgaagtccg ggaattttcc
     9061 ctgtcaccct cagacgctcg ccgtctgcca ccggcatttt ttccggccgg aacagcgacc
     9121 agctgctgtc cagggaggaa atacgcacca cctgcgtttc gccctgcgca tcccgcaggg
     9181 tcaggctgtg actctgcgcc gtcacccggt cgataacata gcggtcatga ctgcgtgtct
     9241 ccgggttcca ctgctccatc accatccccg ggcggtacat atcccgcaga taacggctcc
     9301 ggctgtccag ccagaccggt gacagggcgg tcatggttac ctccgggcgt ccgagcacgc
     9361 cctgtgtttt cagctcactg cgaatggcct gtgtcagtat ggcctgttcc cgtaccccgc
     9421 tgacctgtgc cacgctctct tctccggctt tcacgctggc agcaaaatct cctgccagcc
     9481 gggcatagcg gacattacgg tccggttcac tgatgatggt ggccggccgc tgttctcccc
     9541 cctgccagcg atatgtgttc accccggcat ctttcatggc catcagtgca ctgcctgtac
     9601 cggttcgctg cccgctgtcg gttatcagga cctgtacgtt atgacgtgcg gcaccgtcca
     9661 gcagggttaa cgtctctttc agggagagtt tttcgccctg gtcaacgata acagtattgc
     9721 ccggcgtgaa ggccatgccc tcctgcagct gacgacgtcc ggttatcagc tcaccggaca
     9781 gccgttcatc ctgcttcagg ttcatctgcg agcgacggtc agcggcgata atctgcacct
     9841 cccgcccctg ctcccgggcc atcatgacca gctccgccac ccgctcacgc tgcccggctg
     9901 caccgccctg cccggacaca atagccagcg acgggcgatc ctgtgccaga acactgaccg
     9961 catcgctgta accggccgtc cgggggacac ttttctccgg atgtacggtc acccggttct
    10021 gtttcatgat gtcacgactg agtgcccgga ctgacagctc atcgagcaca tgaatcccgg
    10081 acgtgaacag ccccttctca cgatcgaggg ggataagctg ctcacggctg atggcctcat
    10141 cgataccggc gcgtgcccgt tcaatcacac cattttccgg cggcagtatg ccgaccgttc
    10201 tggccagcac gtccgtgtac gtaaactgca ctttgcgttc acttaatccg gcaatggcct
    10261 gtgtcaccgc ctgctgcaca tccggcccgt cctgtgaagc gggtccgggc gtctgcgtgc
    10321 gggtctccgc ccgttggtcc gccgcgtcac gatacgcccg gatgtcgaac ccggtttcct
    10381 tcagcgtctg catccattca gccattctga cttcaggatc gacgtgctgt ttggattttc
    10441 gcgtatccag tgccgccaca tcccgggatt tcagcgaggc gtcttccccg acggcctccc
    10501 ggatagcctg tgagcgtccg gagaaggcct ccaccggtac gcccggcatc tcccacatac
    10561 cgtgcttacc aaccacttcc gtttcatagc ccagcgcctc aacctgctct ttcagttttt
    10621 cccggtagag cctgccgaag gcaatctgat tagcgtacac attctcaatg aaccccgttt
    10681 tccccacttt gtcactgctc agtgtcttcc actcgccgtt atgctgcgtg acattggcca
    10741 ctaccgcatg tgtgtgtaac tgtggttcct gatcgcgact ggtgttgtgg ttaaacagtg
    10801 ccatcacaag attaccggtc agcaccgttt ctgactgtcc gtccgtcata acccgtgtgg
    10861 aagccagcgc ctccacctga cggacagcaa aatccacggc ctggttatgt gcatcaataa
    10921 gacgtttatc accgcctaac atggccatca cggagacact tttgggggcg gagaaggtca
    10981 ggtcgtagcc gggacgatgc ttattactgc catcctgcat gcggcttaaa tccgctccgt
    11041 ccggcagctt cccctccaga agacgggtaa aaacatcctt atcgacgctg ccctgcagcc
    11101 ccagctgttc agcccccctg ccggcccagc gttctcccat gctgcccagc acatagtaat
    11161 tatccttgtc ggtataatag ttaccggcac ttccggccga tctgacctgc gcgatactca
    11221 tcataaacgt atatcccttt gacatatccc ggtatcaatc ccacaatgga tatacacaag
    11281 acatatccac ataaggaggc aaataatgga aaccaccgta tttttcagca accgcagcca
    11341 ggcggtcaga ctgccaaaag cggttgcatt gccggaaaac gtaaaacgcg ttgaagtgat
    11401 tgctgtcgga cgaaccagaa tcattacgcc agccggagag acatgggacg aatggttcga
    11461 cggacacaac gtcagcgccg attttatgga taacagggaa cagcccggca tgcaggaaag
    11521 ggagtcattc tgatgctgaa gtttatgctc gataccaaca tctgcatttt cacgatgaag
    11581 aacaaacccg ccagcgtcag ggagcgtttt aacctgaacc aggggagaat gtgtatcagt
    11641 tcggtcaccc tgatggagct gatatatggt gcagaaaaaa gccagatgcc tgaacgtaat
    11701 ctcgctgtga tcgagggatt tgtttcccgc attgatgttc tggattacga cgctgccgca
    11761 gccacacaca gcggccagat aagagcagaa cttgcccgtc agggacgccc tgtcgggcca
    11821 tttgatcaaa tgatcgcagg tcatgcccgc agtcggggac tgattattgt gactaataac
    11881 acccgggaat ttgaacgtgt ggacggtctg agaattgaag actggagctg acctgttcat
    11941 cagaaatcat ctcccggctc aacattctcc ccgcgctccc ggtgcacatt gatgttcacc
    12001 tcttcacgac gctgcatctt ctgctggata tccggattct gctcccgttg ccatgcctca
    12061 taaacggcca tatccaccac ttcaccggat tcactgatcc cgggtggcag ttgctgttcc
    12121 atctcttctt ccggtttcat tttcagctcc tgctcgatcc cccctgccgg aacattcaca
    12181 cctgcatctg acttcttatc gttgatggca ggagacaccg gctgttgcgg ctgttcagcc
    12241 tgagtcacgt cttctccgga aacaacctcc gggacatccg gttcgaagag gctggccatc
    12301 tcacgacctt ctgcttccct tgcggcaagt acggcactca gacggttctc catttccggg
    12361 ttgatgtcac gcggaataaa ctccggagcg accttcggtc gtgtctgata tttcagggag
    12421 agttttactg ccggatacgg tccgggcagg gtgacataac aggtcagatc cggcagagac
    12481 tgaatgtcgg aataactgac cagcgtctga cgctccatat ccttaccggt cgataccccg
    12541 tcacgtaccg gatcagcacc gtaggaatac tgctcgctgg ctttcaggtg ctctttctca
    12601 ccaatttcac cggcagcgaa ctctgcaatc ttatggctgg gagaacggaa aaaggcacgg
    12661 gtgttcatga cgtcaaacag cgtggcagcc gctttctcac cgtagatatc ttccagctgg
    12721 gcataggact ggataccaaa cacataacag ccaccgaact tacgggcttc cggcaggatc
    12781 tccaccaggt ccggcagttt gtgtaacgtg ggtaactcgt cacagaaaaa ccacacacga
    12841 cggttacggt tttctcccat tgccagcaga ccacgaatag caatggacag ccacatggag
    12901 atcaccggct tcagggaggc atgggtgtcg gcgttcgacg aaataaacag ccagccgttt
    12961 ttctgatctt cccggacacc acgcatccag tcacggatgg tgaagggctc accgttatgc
    13021 tcaatcccct gcaggtaacg gatggctttc acgtagttgg tcagcacagc acggatggat
    13081 atggccgttt tctcgatttt ctcttccacc aggttggccg ccggtgaatt acgcaggaag
    13141 gtacgcagct tttcaatttt gatggaaagc agtgtgtcca ccagtttgct gtagctgcgg
    13201 ttggggtcat tacgcatcag gtatgccgct tccgcaaaaa tggtacgtcc tgaaccctgc
    13261 cagaacgggt cctcttttgt ccccatcggg atcagagtat ttgcggtatt atcaaaatct
    13321 ggctgtgtca ggcactcctt ccacaaatcc caggcagcac agcgtgcatc cagcggattc
    13381 aggattttat cgatggaggg atcatagtaa cttttaacaa attcccccga acggtcataa
    13441 atcaccacca tatcaccacg ctgacgggcg tagttggcca gacgacggat aacctccgac
    13501 ttaccggccc cgacagtgcc gtgcagacag aagttctgga tttcagaatc ccggataatc
    13561 ggcagatcgc caatccggat atcggaatcc ttgccatctt ttttcagcat cctggccacc
    13621 tctttcggat tgtcagtcag ctgacgacca cctgtgattt cattttcgct ctgttgttta
    13681 ccctgacgcc ccagaatcca ggagacaaca aagaaggtga tcaggcagat aaccagggcc
    13741 acgacagacg ccagaacgaa tgccgaccat aactgctcac cgcaccagat catatattta
    13801 tcatgcagta cctgagccgc gttcatccgg aaggttttgc cgtaatactg gatctcatag
    13861 accggctggg acctgattaa atcccgcatg ccttccagcg tggtgcacca ccagtaaatg
    13921 cagccattca caaacgtctg ccagcttatt tttacccata aaaccagacc aactaggatc
    13981 cagaaaaaaa taaacaggca gtaaagtatg atattggcga tctgactgaa catgcggata
    14041 cgcatggacg caatctgacc gccctgggtc atattctttg cgttaaaact catgatgata
    14101 ttctgaagaa agaaagtcag tcggactgat tattaatgat gaaaagatta ataaccgtta
    14161 aattccggct gcgccggcaa cacttcacgg tcgcacaaag aatttgcaca accgctcagt
    14221 gttttcatat ttatttcgga aacgccgggg agaaataaaa tttattttat ttctccccgg
    14281 ggttggacaa cgagcaaagc gaggcgtcag tcaggaggcc ggtcagacca gcctccggaa
    14341 gataatcaga gaatatttgc gattgatttg gccagctggt cttcgagaac aggcttcgcc
    14401 tcttcaaatt tcaggttaac cttgttcgca tttgagacca cacgggtctg gtatttatgc
    14461 tggttacctg tttcagtact ggtctgaatt ttcgcacctg atgtgccctg acgcagggcg
    14521 gcaacattat ccgttgtcac cgttgcctta gtacgctctg caatctgcac atccgtgatc
    14581 atggtatagt tcacatcttc caccatcgca tctgcagcca tacccaccag accggcagca
    14641 aggcccacac cgagtgtggc accggcagaa ttggaattat atccggtgat accggcacct
    14701 aacgctgcac caactgctgc gccttcataa ccacggttca gccatccctg agactcccgc
    14761 agatccatct tatcggcctt cagcacattc gcctgaatcc agtagtaggc tttatccgga
    14821 gaagtcacca cctgataccc ttttgctttc acagcatcag caattttgcc ctgcagccca
    14881 ctcatgtctt tatcagacgt gtttttgaac tgcagaaata ccgtgcgttc gctggcgggt
    14941 tcaagccaga tggtctcact catctgagtc ttcacctcaa ggttacgctt cttgattgct
    15001 gtgctcatcg caccacaccc tgaaagggcc agagtggaac tgaccagtgc aaccatcatc
    15061 aatttttttg ttttcatcca aaatatcctt ttaactaaca gactcttttt tgaacagccc
    15121 tattactgag g
//
LOCUS       DAJOTB010000116        15032 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJOTB010000116 DAJOTB010000000
VERSION     DAJOTB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.116"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            24..1211
                     /locus_tag="OHN28_003530"
     CDS             24..1211
                     /locus_tag="OHN28_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCQ2298860.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LHSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNTSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            1208..3148
                     /locus_tag="OHN28_003531"
     CDS             1208..3148
                     /locus_tag="OHN28_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HCQ2298861.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            3152..4522
                     /locus_tag="OHN28_003532"
     CDS             3152..4522
                     /locus_tag="OHN28_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HCQ2298862.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPALKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            4996..5190
                     /gene="tnpA"
                     /locus_tag="OHN28_003533"
                     /pseudo
     CDS             4996..5190
                     /gene="tnpA"
                     /locus_tag="OHN28_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
     gene            complement(5319..6260)
                     /locus_tag="OHN28_003534"
     CDS             complement(5319..6260)
                     /locus_tag="OHN28_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298863.1"
                     /translation="MAIINIYSKRQRKIRGEVNDVYQYNNIPHALRVQIIKIITDSIG
                     FPSSNERYTSYRNEADKVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEK
                     CLDFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIR
                     IDSQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIM
                     KAIHDKNNWKYSPNDTASKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAG
                     HGQGADIKEVPEELVSYMLHLTATNLLFLLKCEKNIK"
     gene            complement(6495..7884)
                     /locus_tag="OHN28_003535"
                     /pseudo
     CDS             complement(6495..7884)
                     /locus_tag="OHN28_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(8194..8343)
                     /locus_tag="OHN28_003536"
                     /pseudo
     CDS             complement(8194..8343)
                     /locus_tag="OHN28_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8521..9714)
                     /locus_tag="OHN28_003537"
     CDS             complement(8521..9714)
                     /locus_tag="OHN28_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCQ2298864.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VTRLS"
     gene            complement(10169..11397)
                     /locus_tag="OHN28_003538"
     CDS             complement(join(10169..11082,11082..11397))
                     /locus_tag="OHN28_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCQ2298865.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCSDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     TESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            12104..12397
                     /gene="iss"
                     /locus_tag="OHN28_003539"
     CDS             12104..12397
                     /gene="iss"
                     /locus_tag="OHN28_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HCQ2298866.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(12455..12596)
                     /locus_tag="OHN28_003540"
                     /pseudo
     CDS             complement(12455..12596)
                     /locus_tag="OHN28_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            13207..14306
                     /locus_tag="OHN28_003541"
                     /pseudo
     CDS             13207..14306
                     /locus_tag="OHN28_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4010 a         3508 c         3523 g         3991 t
ORIGIN      
        1 cattgttatg gaaaacatgg acaatgttaa tgaaatatat ttctcaccgc gccatcattt
       61 gtaccttgac actgttgatc attattatca ccgttttgtt cacctttctg agaagctctg
      121 atgtgccaga atatattact gccccggtgc gcaaaggcga tatagaaaat tctgttctgg
      181 cgacaggccg tattgatgca attgaacggg taaacgtagg ggcacaggta tcagggcaat
      241 taaaatcact gaaagtgaaa cagggcgacc atgtgactaa agggcagttg attgccgaaa
      301 ttgacgacct acctcagtgt aacgatttgc gtaatgccga agctgcactc aacgaagtga
      361 aagcagaact tcaatccaag caggcgctcc tgaaacaggc tgaactgcgt tttaaacgcc
      421 agctccggat gctaagagaa aatgccagct cacacgaaga ttttgagtct gcggaggcaa
      481 tgcttgcgac cactcgcgct gaattacatt ctctgaatgc caaactggtc caggcacaga
      541 tagaagtaga taagaaaaag cttgcccttg aatatacgcg agtcgtagcg ccaatggatg
      601 gaattgttat tgccattgtc actcagcaag ggcaaaccgt taactcaaac cagagtgccc
      661 caacaattat taaactcgct cgtctggatg tcatgaccat taaagcacag atctccgagg
      721 ctgatataac ccgtatttct gtggggcaaa aagcccgctt ctctatcttc tccgaaccgg
      781 acaagcacta cagcgcaaca ctgcgtgccg ttgaactggc accagaatcg gtaatgaaag
      841 atgactctct cgccagcaac acttcagcat caggctccgg aacttcaaat gcgtccgttt
      901 attacaatgc tctattcgat gtgcctaacc cagaaaatcg acttcgtatt gccatgactg
      961 ctcaggttac cctgattact gatgaagccc aaaatacact gctggtaccc attcaggcag
     1021 tgcacagaaa tgaggggaag aagcaacagg tcctggtgct ggcagcagat ggcaggctgg
     1081 aaccccggaa tgtgaaaact ggtatcacga acagcgtgga tattcagatc ctcgaaggtt
     1141 taaatgtcgg agaaaatgtc gtgctgtcgc tgccggataa gaaagagccc gaagaaagga
     1201 ttatgctgtg aaaaaactta ttgagttaaa aggggttagc cgaacttatg gcaacggaga
     1261 tcaaacgcgg accgtcctga aaaatgtcga tttgacaatc gttgctggag aaatggtggc
     1321 aatcattgga gcctctggct caggcaaatc aaccttaatg aacataatgg gttgccttga
     1381 cgtacccaat agaggtgatt actacatcga cggacaaaat gccgcctgtc tttcacccga
     1441 tgagctggct agagtgcgcc gggaacacat tggctttata ttccagcgct accatttgat
     1501 acctgatctt agcgcacttg gtaacgtgga gatcccggcc atttacgcca acagcgaacg
     1561 cgacagccgt cggcaacgtg caacggcgtt gctgggcaga ctgggactgg aaggacgtga
     1621 gcatcataaa ccttgcgaac tttcaggcgg tcagcagcaa cgcgtcagta ttgcccgagc
     1681 actgatcaac ggtggaaaga taattttggc ggatgagcca actggtgctc tggattcgca
     1741 atccgggcaa gaagtgttag ccattctgaa tgaacttaat cgacgtggtc atactgtagt
     1801 aatggtgacc cacgatatga aggtcgcccg gcacgcaaag cgaatcattg agctgtgtga
     1861 tggcgaaatc atcgctgata gtggaggttg cgtatctgcg acggaaacac taccaaaaac
     1921 caacagaatc cggcaaagct attggaaaac actgctcgat cgaacgcgtg aatcgatgca
     1981 aatggcgcta aaagcgatga agacgcatcg cttacgcacc acgcttacca tgatcgggat
     2041 tgtttttggt atcgcctccg ttgtcactgt tgtggcattg ggtgaaggag ccaggcaaga
     2101 aacactggaa gagattaaaa gtctggggac aaacgtggtc agcatttatc ccgggcagga
     2161 tttgttcgat gacagcattg agagtattag aacattggta cctgctgacg caaatgcact
     2221 ggcgaagcaa gggtttatcg atagcgtcag cccggaagtt agcgcttcag acaacattcg
     2281 ttttctgggt aaatctgcga tagcgtccat taacggcgtt gggcgggaac atttccgggt
     2341 aaaaggtatt gagttgttgc aagggactac ttttagagac gatcgcaacg ctctacagga
     2401 agttattatc gatgagaata ctcgtaaggc cattttcgac aacacagggt tacaggctct
     2461 ggggcaaata gtctttcttg gttctgtacc agcacgagtg gttggcatcg ctaaaagtaa
     2521 caaccgtagt gatgcatcaa atcgcattac tgtgtggatg ccttacagca cggtgatgta
     2581 ccgtattgtg ggtaaaccag ttctgaccgg tattagcgtc aggctaaaag acaatgtgga
     2641 taacgaggct gcaatcagcg ctatctccca actattaacc cggcgtcacg gcataaaaga
     2701 cttccagctt tataactttg aacaaataag aaagtctatc gaacacacct caatgacctt
     2761 cagtatttta attcttatgg tcgcatgtat ctcactaatg atcggcagta tcggggtgat
     2821 gaacatcatg ttgatttctg taacggaacg aactcatgaa attggcgtac gaatggcggt
     2881 tggcgcgaga cgcagcgaca ttatgcaaca gtttattatt gaggccgtat tggtttgcct
     2941 gattggtggc gcactcggta ttgcactgtc gtatatcaca ggtgcgctct ttaacgctct
     3001 ggcggacggg atattcgcag ctatatattc gtggcaggct gctgttgctg catttttttg
     3061 ctcaacctta atcggcataa tcttcgggta tctcccagcc cgcaaggcag caaggatgga
     3121 cccggttatt tcactggcca gcgaataata tatgaaaatt ttatcatcgt tgactttatg
     3181 ccttatcacc gcactctgtt caggttgcac aaacgtgctg aaaagtgact atcgcgctcc
     3241 cgaagtgaac tatcccatca actggaccaa gggcgatgtg gacggaaata catccccgtt
     3301 tgactgggaa gaatttaacg atcctaatct cgataactgg cttcacctgg tgatgacaag
     3361 taataacgac atagctatag ctgcactgcg gatacatcgg gcgcagctgg atgcggaaag
     3421 aacaggtatc accaatactc ctgcactaaa ggcggcactg agtatggacg gaaaaaaaca
     3481 gctgaataac tcgtccggct gggcaaaaag cggttctgcc agccttggca ccagctatga
     3541 gctggatctc tggggaaaaa ttgcccgtca gcgtgatgtt gctgagtggg cagtccatgc
     3601 cagtgaagaa gattttcgct ccgcgcgcct gatgctgctt tcagaggcca gcaataatta
     3661 ctggcgtatc ggttttgtaa atcaacaaat taccaccctc cagcagagta ttgactatgc
     3721 aaaagagacg ctgcgtctgg ccgaggttcg ctatcgtgca ggaaatatct cgtcgctgga
     3781 tgtgattgac gcgcagcaaa acctgctcac gcaagagaac cagcttacag ggctacaacg
     3841 agaacattca cagctactca atcagcaggc cgttttgctc ggcaccgtgc ccggttgcca
     3901 gatagtcgag cccacaacgc tgccaaaagg aagcctgcct aaggtcaatg cgaatattcc
     3961 tgccagcata ctgatgcgcc ggcctgacat aagtgctaaa gagtggcagt tgcgtgaggc
     4021 gctagctacg gttgatatca agcgtagcga atattacccc accttcaacc tgacaggcgc
     4081 tttgggtacc agcagcgctt cgctactggc attactgcat aatccggtcg gttccgtagg
     4141 cgctaacctg acgctaccgt tcctggagtg gcgacagcgg gacatagagg taaaaattgc
     4201 ccgtaacgat tacgaacagc gagtgctgga gtttaaacag ctactttata aggcaatgag
     4261 cagtatagag gacgcgcttt catttcgtaa ccagttgctg cttcaggaga caagactcag
     4321 ggaagaactg gaacttgcac gtaagtcaga gtggttaaac gaagttcgtt accggcatgg
     4381 tgcagtacgt atttcattct ggcttgatgc tcaggaaaaa cgtcgtcagg cggagctgcg
     4441 actggatgaa aaccgcttca atcagttgca gaatctagca aaaatttatc ttgagtttgg
     4501 cggggcatca acctttcctt aagtctaatt ggggacaacc aggagcaata aggagtgaaa
     4561 aatagccaca gctgaaaaca ccatagccat tgacatttga gctttacctg ctcttgaaaa
     4621 caaaaaatca ctgttaagcg caatgctatt cacttcagca gagcagtatt gcgtgtcacg
     4681 agcatgttta ccattctgtg taaatatctt ttcttaaaag tgatcatcaa agcagtcatc
     4741 aaactcgata aatatccacc ggcatagcgt aatgccgagc aattaaggat caattgaccg
     4801 ctccttaaac tgcggcacta taacggcttc tacaacaggg agccgttttc ttgtgcatag
     4861 aaaaccccca gctaggctgg gggttccgga aagctttcag ctttgagcca gttattaaaa
     4921 ccccttttga tttgttaaaa caccttgcgg tctggcaact gcaagtgtca aacaagaaat
     4981 caaaaggggg tcccaatggg gaacgaaaag agcttagcgc acacccgatg gaactgtaaa
     5041 tatcacatag tatttgcgcc aaaataccga agacaggtgt tctacagaga gaagcgtaga
     5101 gcaataggct gtattttgag aaagctgtgt gagtggaaaa gtgtacggat tctggaagct
     5161 gaatgctgtg cagatcatat ccatatgcta caatgtttgt caaagctcag tgaaaatgtc
     5221 taatcactgg ctcattataa atgtccatat attgaaaagg ccgctgcgaa aatccctccc
     5281 gcagcggcct ctttagcata acgtcattat gcgaaccatc acttaatgtt cttctcgcat
     5341 tttaagagaa atagtaaatt tgtagcagta agatgaagca tatagcttac caattcttcc
     5401 ggaacctctt taatatcagc tccttgccca tgacctgcat ttttgtttct gatggttgga
     5461 attcctgttt ccagcattgt tttcagactt gtgaactggc tttgtaaata tgccggaatt
     5521 aaattttgac ttaaacagct atttattagt tttgatgctg tatcattagg actatatttc
     5581 cagttattct tatcatgtat tgctttcatt atactttcaa atgatttcag acaatcattt
     5641 agacattctt tataacgctt atgcctgtaa tgctcatgcg cagcaaggaa ttcatcattg
     5701 gcgccttcaa acaagggttc tccacttaaa agaatcaggg ttggctttac gacatcagca
     5761 tgaatcaatt gagagtctat ccttattatc tcttcagatt caaactgata accaacccca
     5821 tgttctctaa acctttcatt tagttcaata actgcatctc caggactttg acttgttatg
     5881 tctttgaatt cataatggtt tttcgcaacg tggctcacta gtatctggaa gcatatttct
     5941 atgaaatcta ggcatttctc tgtatttctc tctttaagga aaaagtcgac aagagcgtca
     6001 aagtcattct ttgcaaattc cttcaaagaa aaaacaccat attccttaga aagtatctca
     6061 tggatatatg catatacttt gtcagcttca tttctatacg atgtgtagcg ttcattagaa
     6121 gaggggaagc cgatagaatc agttattatt ttaattattt gcaccctgag tgcatgtgga
     6181 atattgttat attgatatac gtcatttact tcgccacgaa tttttctttg tctcttagaa
     6241 taaatattaa taattgccat gaaatagcct cacgattttt ttacatgctg ttctgtgata
     6301 cttgctgttt ggctctttgc accgcgaagc ggttcggggt tctgtgacac ccgaatttta
     6361 cctcggtttc ctttgtatat aatatcattg aagttatttg catttctcag aaatcttctc
     6421 tggctttgat tgcaatagcc gaaagatagc gcacacatcg ccgttggtca gttagtacga
     6481 ggtttccgcc aagttcacct tttaccaaag atggcctcag agcttccctg aagctttatc
     6541 atggcttcaa gtaggtcatc ttcgttcagt gagcgctgag atttgttgat gctaggggcg
     6601 tgccgtctgc gggaaggctc atcaccagaa caaggtaacg attgtgagcg attattgtcc
     6661 cgttttctct ggacctgacg ggcaacatcc aacacatggc caagtctctt gttatctaca
     6721 atggcgacgg gatcaacctc agaaagccgg tcataggtgg agtagggcag cagcacgcca
     6781 tttagtcgca gctccttatg tccgtcagga taatgccaga catcaatata cttgccgatg
     6841 gctcttcgac tgtactcgtt gtcctcaatc agatagagca ttttatcgta ttgtacagtc
     6901 agattttttg atacccggcg aggctctcgc taggtaaatt ctgcatcaag gtcatcgtct
     6961 aatgccagcg gccggtgcgc gttaaaatca tgcaagggtt ctttggcaaa gcgccggtta
     7021 taatccttct gcaaatgcat tggccgcttc tattgtacag atccccttaa gcctgagctc
     7081 cttaacgagt cggtcctgaa gtgtcaaatg agcacgttca acgcggcctt ttgcctggct
     7141 ggtttcagca cacaaaggag taatattaag gcagcgcatg gccctggcaa actgggtttc
     7201 gccggggccg gtggtggcgt gcttattatt gactctgaat acgctggctt tatcgctgta
     7261 gaggatcatc ggtttaccgt atttctcgat atagccacgt gtagcttcaa aataggtaaa
     7321 tgtggactca gatttcacaa acagcaggtg catcaggcga ctggtggcat cgtcaacata
     7381 gaccagggca gaacagggtc gaccccggtt ttcaaaccag tggtggtcgc agccatcaat
     7441 ttgtatcagc tcgccagtac aaggacgccg gtaacgaggc tgatgaattt tcggtgcgcg
     7501 ttgtctgcgg ggaatccaga gtccggcctt tatcatgaga cgtcggatgg tttcctttcc
     7561 aaggaccaga ccgtgaactt cctccagctt ttcccgcgct agcgtggggc caaaatcacg
     7621 gtagtgctcc cggattatac tgagtgcctg atctgtcagt gacgctggca acagacggtt
     7681 accagtgcgg ccacgactct ggttgttcat gccaagcggc ccatactggc gataacgttt
     7741 gagcagacgg ctgcaatgac gtggcgtgat gcccagcatt tcggcagcct gtccaggacg
     7801 gatattgcga tcgatgacat cctgaaggat tttgaggcga ttgatttctt tcacggtgaa
     7861 cattccggcg tcatttatag ccatagaaaa ctcctcatca ggaaaaagtg agtcgttgtc
     7921 tagacatagg gatctgagaa actatagcct ggacattcct aatgagccac aaatagacat
     7981 tagcattgag cctctacaat gtttgtgcgc acaatctatc ttatgttaaa taacattgaa
     8041 tattaactta cttcatgaag cttactgctt ctatttccac ttctttttct tattaatcgc
     8101 tcccctaatc cacgcattca tctgctctac aggctctaaa atattttttc tgagtactga
     8161 gcacaaactg ctccataaaa aacagaccac aagtttgggc atgatactga tgtagtcacg
     8221 ttttattgtt ttcatgaagc tctctgctat tccattactc accggactcc gcaccgccgt
     8281 gttcttcgat ttaagtccca acatccgagc gaactggcgt gtttcattaa cccggtagtc
     8341 agagtcagcg gtcgaaagac ctcccaggta gctactctgc atacccacta taatgcaact
     8401 attctcgtga cgtgtgtttc agctctcccc cggctatgat acgagatggt atggagagcc
     8461 cttctggggc cactgcctgc accagatgca acaggaagcc tgatatgaat gcaatttctg
     8521 tcaggacagg cgggtaactt tctgtggcca tggatcaggg aatactccac ccgacatcca
     8581 ccactgaatt tcttcttcag tcaacaagca aagggataag gcatgtgtaa aatcattaac
     8641 ttcagtggca ttgccaatga tagtcaactg acagcagcga tcgccaaatc gcccagaggc
     8701 tgtgtcgatg tgtcgctgta gcgctgaacg ttcttcacga gttaaattat tgtcagtatg
     8761 ttccagtact ccagctttcc agtagccaat gagcgccaga ctgatgcttc ctgctgactg
     8821 attccagagc agggcaagat catctctccc ggggagccaa aaaaaacctt tactgcgata
     8881 aacgcccatg cccataaagc ggtgacaggt atcccataat ctttgcggat ggaatggcct
     8941 gtcgtccctg atgacgcgcc atataagttc ctcattgttg cacggagtat ccatgtttcc
     9001 tacaagtgct tccagttcac tgatgagtaa ctcaacacgg tcaaaattgt agtccgggat
     9061 cgccagtaat gtgctcagtt caatatttcc ccatgatacc gcgagcacat ccacggaggg
     9121 gttaagtggg tgtatggctt ttgccacagc ggagataatg tcaaagggca ggcggtcatt
     9181 tctggtcagc agcagcctgt tgcagaacat gatttgctcg gccagcaaat tctccaggcc
     9241 gcgtttgtta tgccggagat tttcctgaag agtggggatc agttttttcc catcattgta
     9301 atcttcatgg agcatgacag tatcaacgag tgttaaaaac gccttcaggc gtacacgtga
     9361 atggcgacgc aggtatttga ccagaggaag cggatgactg cttccggagg tttcaatcag
     9421 catcacatcc ggcttgttgt gatcaagcag gtggttaatt gcgagatcca tctttttgag
     9481 gcctgcgggg ctgctgatgc tgtctgcggt gatacttacg aaattattgt cttttgcatc
     9541 tacaatatct gtgttggcga tcagcacgcc gtccacatcc agttcgctca tgtcattgac
     9601 tatgacagag actgaaagct gtttttcgtg tgcctgtatg agcaggtttt tcagcagagt
     9661 ggttttccct gcgccgagga agccattgag aatgatgaca gatgttgttt ccatatcagc
     9721 agtacaccag ttagttagcg aggaaggcgc ttggtggtac aaagagcatc cggattgaat
     9781 cgcataacgt cgacgggagt tgttgtcgtt tttcctgtga ggtctcaacc tgccacagaa
     9841 cgcaccggat ttattggtat ttagtctcca tcagcataat tatcaccatt tttatctccg
     9901 ggggaagatt tgttctgaac aggagtggga ggtgtgacct cctgaaagta attacagatt
     9961 gctagttgtt gtatttttat tcgaatatgt ctcttcgtat cagtgatagc acctagataa
    10021 agtatattcc gggagcgtta tctgttctag atatcagaga gtcagtcatt tgtaaaaaag
    10081 agagctgcat cattctattt ttcctgtcta ttcaccctgt tgtcgtatct aaaagacatc
    10141 cgttccgagg atgtcggtgg atttgcccct atatttccag acatctgtta tcacttaacc
    10201 cattacaagc ccgctgccgc agatattccc gtggcgagcg ataacccagc gcactatgcg
    10261 gatgccattc gttataatgc tcgaacgcct ctgcaaggtt ctttgctgcc gttaacccgt
    10321 ctggtttggg catgatactg atgtagtcac gctttatcgt tttcacgaag ctctctgcga
    10381 ttccgttact ctccgtactc cgcaccgccg tgttcttcgg ttcaagtccc aacatccggg
    10441 cgaactggcg tgtttcatta gcccggtagc atgaaccatt atccgtcagc cactccactg
    10501 gagacgacgg aagatcgttg ccgaagcggc gttccaccgc tcccagcatg acgtcctgta
    10561 ctgtttcact gttgaagccg ccggtagtga ccgcccagtg cagtgcctca cgatcactgc
    10621 agtccagcgc gaacgtgaca cgcagtctct ctccgttatc acagcagaac tcgaacccgt
    10681 cagagcacca tcgctgattg ctttctttca cggccactct gcctgtatgt gcccgtttcg
    10741 atggcggtac agcaggtttt cgctcaagca acagcgcatt ctggcgcatg atccggtaaa
    10801 cacgtttggc attgatcgca ggcataccat caagttctgc ctgtctgcga agcagcgccc
    10861 atacccgacg ataaccatac gtgggcagct ctccgataac atggtgtata cggagaagca
    10921 catccgtatc atcagtgtga cgactgcggc ggccatccat ccagtcatcg gttcgtctga
    10981 gaatgacgtg caactgcgca cgcgacaccc ggagacaacg gctgactaag cttactcccc
    11041 atccccgggc aataagggcg cgtgcgctat ccactttttt gcccgtccat attcaacggc
    11101 ttctttgagg agttcatttt ccatcgtttt cttgccgagc aggcgctgga gttctttaat
    11161 ctgcttcatg gcggcagcaa gttcagaggc aggaacaacc tgttctccgg cggcgacagc
    11221 agtaagactt ccttcctggt attgcttacg ccagagaaat aactggctgg ctgctacacc
    11281 atgttgccgg gcaacgaggg agaccgtcat ccccggttca aagctctgct gaacaattgc
    11341 gatcttttcc tgtgtggtac gccgtctgcg tttctccggt cctaagacat caatcatctg
    11401 ctctccaatg actagtctaa aaactagtat taagactatc acttaaataa gtgatactgg
    11461 ttgtctggag attcaggggg ccagtctatt ctccttcacg gtaatacgca gttatagttg
    11521 cagagcaatg taatggatct tttatattaa gaaattgtgt gagtggagag gtgcacgaat
    11581 cctagaggca gaatgtttct ccacatgctt gtggagaaac cgccgcattt tgcccgttct
    11641 gtacgatact ccgtagccag agataagata ttctgtcgct gtatgctaca tacattacac
    11701 tatacgaaat caggtctgtt caatcagtaa ggaaataaac ggacaaacgt tatgtaaccc
    11761 agacagagat atattgtctg ggaaaatagt ggcttttgtg gcgaaaacta gtaaaacagc
    11821 aacccgaacc acttgatgtg catcgttttt gattattccc gtatactctt gcagaaggag
    11881 ttctccgtcg ggctactgtc atggttaatg cggggaatat ggcgacaata caacacacct
    11941 aaaagagtaa tggacagatg aagcggttta ttcatttccc atgattctga gtacctacca
    12001 agtctgagta accactttta tacttttaat tttcgttcat ttagctatcg tttaattatt
    12061 atcacatagg attctgccgt ttttaacaat gcaggataat aagatgaaaa aaatgttatt
    12121 ttctgccgct ctggcaatgc ttattacagg atgtgctcaa caaacgttta ctgttggaaa
    12181 caagccgaca gcagtaacac caaaggaaac catcactcat catttcttcg tttcgggaat
    12241 tggacaagag aaaactgttg atgcagccaa aatttgtggc ggtgcagaaa atgttgttaa
    12301 aacagaaact cagcaaacat tcgtaaatgg attgctcggt tttatcactt ttggcatcta
    12361 tactccgctg gaagcccggg tatattgctc acaatagttg cccatcgata tggggagctc
    12421 atctgcactg ttcattaata tacttctggg ctccctacag ttgtttttgc atagtgataa
    12481 gcctctctct gagggaggaa ataatcctgt tcagcgatgt ctgccagtcg gggggctgca
    12541 ttatccacgc ccgaggcggt ggtggcttca cgcggggatg ggcagattga tctgatatgc
    12601 aaccgacgac gaccagcggc aacatcatca cgcagagctt cattttcaga tttgggccac
    12661 cttttgattt ctcgtttaac ttttgcagtc accagactgt gatttatttt aacaaatcaa
    12721 tagctttcat aaaattctcg aagctgaaag cttaactgaa ccaaccagcc aggctggtgt
    12781 tttctaaaga caaaaaggcc atcagcaaaa cactgatagt ctgaatcacc tttatcaatc
    12841 atgtatgaag aaaatatgcg caattagttg acagtgatta tcattttcat taaaaagagc
    12901 gcgtagcatt cttatttcat gaggaaattt acccgccaac aacctgagta gcgaaagctg
    12961 ttcatcccca atatttttgc tggcgggttc ttttttctac tgtcctcctc cccagaggaa
    13021 gcattcccat taatcattac tggcgaggag gcaagagaaa aagtcggttc tttgtcccta
    13081 aataacagca tcatttcagt ccatgcaaaa tctgatttac cagctcacat aataacctga
    13141 tgatacacag tgtaagttca cagagatatt gcaattgcct ccggataagt aaggggagat
    13201 tgcactatgc aaatgcagca cctgaggttg gctatcctaa gcagccgatt atgcgttgag
    13261 gctttcagtg atggctgata tagcaacaat gagaatgaag gctctgcact tctgggataa
    13321 acacggtatt tctgcagctt ctgaagcctt tggcgtatcc tgccgcacgc tttactggtg
    13381 gcgtcagtta ctgaacaagg gaggacctga ggggctaatc ccgcacaata aggcacctct
    13441 ggtgcgacga aaaaagcact ggcatcccga tgtgctgaaa gagattcgac gcctcaggac
    13501 agagctgccg aacctcggta aagagcagat ttttgttcgc ctgaagccct ggtgcgcata
    13561 acgccatcag acctgcccga gtgtttccac catcggcaga atgatcgcca ccgcacacga
    13621 taaaatgcgg atgataccag tacgtctgag ctcgcggggt aaggctctgc ttgtcaaaaa
    13681 gcgaaccacc aaccccccgc aggccaaagc actaccgtcc ggtaaagaca ggagagctca
    13741 ttgggatgga cgccattgag ctcagaatgg gtgaaatgcg tcgctatatc attaccatga
    13801 tcgacgaaca cagcgattat gtactggcgc tggctgtgcc gtcgctcaac agtgatatcg
    13861 tcaatcactt tttcagccgt gcagcccggc tgttccctgt cggtatcagc cagatcatca
    13921 cagataacgg gaaagagttc ctgggaagct ttgacaaaac gctgcaggaa gccgccatca
    13981 aacacctctg gacctatccc tacacgccaa aaatgaacgc tatctgtgaa cgttttaacc
    14041 gggcattaag agagcaattc attgaattta atgaaatttt actctttgag gatctggcgt
    14101 tgtttaacct gaaactggcg gaatatctgg cgctgtataa cagcaaaaga ctccacaagg
    14161 cgctcgcact aacgacgccc gtggaatata ttttaaaaga gaacaaaaat tgcaatatgt
    14221 ggtggcccca tacacagcaa acgcacaggc cgccccggtt gggtagatat gtgggatggc
    14281 tggttcagac ttcaggatat ggttgaggct ggtgatgaag tctctttatc aggagatctg
    14341 atcaagagac agccgcgcag ggggattcac tatgctggat tcttttaaca aattctccgc
    14401 taaccaaaaa atagactttt tgcattcgta aatgcgttaa gagataattt ttaaagttcc
    14461 atcaatctcc cggccagttc gtctcgcggc attactcact atccgcacaa cttcagtaag
    14521 atcatgactt tttcaatcgt tatctgttga ccaagctttg aaaccactag cgtcccgtca
    14581 acctgacctg gaatcgtcga gaaccgccca tagttgtgaa ttggctagag tgtgatggaa
    14641 agcaacgcta aacgacctag tcacacattc tcttttccct aacaatgacc atttagtgaa
    14701 ataacgtatc caaaatgatg acgtgaaagc ctgacactgg cagcgaaaag ccgaaaaaac
    14761 tcacctggtc aggagcaaaa atcggattat tcgccaacag cgtaatatca gcagtagttc
    14821 tcgacacgct tgcgctatag acagaggatg aaacgctgct ggtcggagtt ctctatacat
    14881 ttttggcgac ggcagaaccg ctcaccaatg ccttaacgtc tatcagcctt ccggtgagtt
    14941 ggaacagcat gctcagcgtt tgcaagatat tctccacggt ctgtagcagc gtaggtttca
    15001 atttaaatat ccccagatag ccctctggga gc
//
LOCUS       DAJOTB010000117        14986 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJOTB010000117 DAJOTB010000000
VERSION     DAJOTB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.117"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(57..2336)
                     /gene="maeB"
                     /locus_tag="OHN28_003542"
     CDS             complement(57..2336)
                     /gene="maeB"
                     /locus_tag="OHN28_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCQ2298867.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2625..3575
                     /gene="tal"
                     /locus_tag="OHN28_003543"
     CDS             2625..3575
                     /gene="tal"
                     /locus_tag="OHN28_003543"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2298868.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3595..5598
                     /gene="tkt"
                     /locus_tag="OHN28_003544"
     CDS             3595..5598
                     /gene="tkt"
                     /locus_tag="OHN28_003544"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2298869.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5675..6718)
                     /locus_tag="OHN28_003545"
     CDS             complement(5675..6718)
                     /locus_tag="OHN28_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCQ2298870.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6844..7419)
                     /gene="nudK"
                     /locus_tag="OHN28_003546"
     CDS             complement(6844..7419)
                     /gene="nudK"
                     /locus_tag="OHN28_003546"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCQ2298871.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7487..9466)
                     /gene="aegA"
                     /locus_tag="OHN28_003547"
     CDS             complement(7487..9466)
                     /gene="aegA"
                     /locus_tag="OHN28_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCQ2298872.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9672..11372
                     /gene="narQ"
                     /locus_tag="OHN28_003548"
     CDS             9672..11372
                     /gene="narQ"
                     /locus_tag="OHN28_003548"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCQ2298873.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11536..14649
                     /gene="acrD"
                     /locus_tag="OHN28_003549"
     CDS             11536..14649
                     /gene="acrD"
                     /locus_tag="OHN28_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCQ2298874.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14748..14807)
                     /gene="ypfM"
                     /locus_tag="OHN28_003550"
     CDS             complement(14748..14807)
                     /gene="ypfM"
                     /locus_tag="OHN28_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCQ2298875.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3502 a         3984 c         3921 g         3579 t
ORIGIN      
        1 tgcagctaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaaa aaagaattac
       61 agcggttggg tttgcgcttc aaccacggcc agcgccacca tgttgacgat acgacgtacc
      121 gatgcgatcg gcgttaacac gtgaaccggc ttcgccaccc ccatcagcac cgggccgaca
      181 gtcacacctt ccgagctgga aacacgcagt aagttgtaac taatgcgggc agcttccatg
      241 ttcggcatca ccagaatatt agcggaacct ttcaaagggc tgtccggcat acggtcgttg
      301 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagttctggc
      361 gcacgttccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
      421 gaaccaaagt tggagtgcga caacagagcc acacgcggct caataccaaa acgacgaaca
      481 gtttctgccg ccatcaaggt gatctccgcc agctcttctg catcaggctc atcattgacg
      541 taggtatcgg caataaaggt gttaccactc ggcaacagca gcgcgttcat tgcacctgcg
      601 gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaagtg ttcatgataa
      661 tcgcccaccg taccgcaaat cattgcatcg gcttcgccac gctgaaccat gatcgcgccg
      721 atcactgtcg ggttactgat cagcgcacgc tgcgcctgct cctgagtgac accgcgacgc
      781 ttcatgatct ggaagtattc ggtccagtac tccttaaagc gcggatcgga ttcgttattg
      841 acgatctcaa aatcaacgcc cgccttgatc tgcaagccca gtttctgaat gcgcatttcg
      901 atcacgttcg gacgaccaat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
      961 gtggcatgta gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt cggcgctttg
     1021 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctccgtcagc
     1081 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc
     1141 gctttcgcca ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
     1201 atgtattccg gaccaaagct cagatcctga tcgccatacg ctgaagccac cacttcactc
     1261 tgttccgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg
     1321 atggcggttg cgccaacgtc cagcgcgcca cggaagataa acgggaagca aaggacgttg
     1381 ttcacctggt tcgggtagtc ggaacgaccg gtacagatga tggcatccgg acgcacttct
     1441 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggcgca
     1501 cgggccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca gccgaggaaa
     1561 atatccgcgc cttcaatcac atcatcgagg gttcgtttgc cgtcatccac caccgcatat
     1621 gcggctttgg tttccgccat gtttggctca cggccctgat agataacgcc ttttgaatcg
     1681 caaaccacga tgttatgttt ttgcagaccc agcgctacca gcaggttcat acaggcgatt
     1741 gctgcggcac ccgcgccaga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
     1801 cgcaagccgt tgaggatggc ggcagtgctg ataattgccg tgccgtgctg atcgtcgtgg
     1861 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
     1921 ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcagcgac gacttcaata
     1981 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc ggcgaatttc
     2041 ttaaacagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct
     2101 aaccccagca ccgccgtacc gttagagatc accgctacca ggttaccgcg cgcggtatat
     2161 ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
     2221 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc
     2281 cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
     2341 ttgttccttt cacgtaacgt tcacaaataa attgtgtggg caacagcccc tgcccacaac
     2401 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
     2461 attaatataa tgttttggta ataatcatat aacactgatg ttacctgctt aatccagcaa
     2521 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
     2581 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
     2641 gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc attcgccatt
     2701 atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc ggattatcac
     2761 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
     2821 aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca
     2881 aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
     2941 agagtattga gaaggcgcgc catctggtgg atttgtatca gcaacaaggc gttgagaaat
     3001 cgcgcattct gatcaagctg gcttcgacct gggaaggaat tcgcgcggca gaagagctgg
     3061 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcacag gcgcgggcct
     3121 gtgcagaagc aggcgttttt ctgatttcgc catttgtcgg gcgtatttat gactggtatc
     3181 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccaggcgtt aaatcagtac
     3241 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
     3301 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga ccgactgact atcgcaccga
     3361 atttgctgaa ggagctacag gaaaaagttt cgccagtggt acgtaaatta atcccacctt
     3421 ctcagacgtt cccacgccca gctcccatga gcgaagcaga gttccgttgg gagcacaatc
     3481 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc gttgatcaac
     3541 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
     3601 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca aaaagccaat
     3661 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
     3721 tttcttaaac ataaccctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
     3781 aacggtcacg cgtcgatgct gctctacagt ttgctgcatc tgaccggtta cgacctgcca
     3841 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa cccctggtca cccggaaatc
     3901 ggttatacgc caggtgttga aaccaccacc ggtcctcttg gacaaggttt ggcgaacgcc
     3961 gtcgggctgg cgatagcaga gcgtacgctg gcggcgcaat ttaatcagcc ggatcatgaa
     4021 attgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatctct
     4081 cacgaagtct gttcgctggc gggcacgctg gggctgggca agctgattgg tttttacgat
     4141 cacaacggta tttccatcga cggtgaaacc gaaggctggt ttaccgacga tacggcaaaa
     4201 cgttttgaag cctatcactg gcatgtggtc cacgagatcg acggccacga tccgcaggcg
     4261 gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc
     4321 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
     4381 ccgctggggg aagaagaagt agctctggca cggcaaaaac tgggctggca ccatccgcca
     4441 tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg
     4501 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
     4561 gagtttaccc gtcggatgag cggcggtttg ccgaaggact gggagaaaac gactcagaaa
     4621 tatatcaatg agttgcaggc gaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat
     4681 acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc ggatctggct
     4741 cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac
     4801 tacattcact acggggtgcg tgaatttggt atgaccgcta tcgccaacgg catcgcgcac
     4861 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac
     4921 gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca
     4981 attggtctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc tagtctgcgc
     5041 ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc ggttggctgg
     5101 aagctggcgg ttgagcgcca caacggaccg acagcactga tcctctccag gcagaatctg
     5161 gcacaggtag aacgaacacc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
     5221 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatc
     5281 accctgcaag cggcagagaa attagcggga gaaggtcgca atgttcgcgt agtttccctg
     5341 ccctcgaccg atattttcga tgcccaggat gagaaatatc gggagtcggt actgccttca
     5401 aatgtttctg cccgcgtggc agtagaagcg ggtattgccg attactggta caaatatgtt
     5461 ggcctgaaag gggcaattgt cgggatgaca ggttatgggg aatcagctcc ggcggataag
     5521 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtactg
     5581 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgatt gtcggatgtg acgctggtgc
     5641 gtcttatccg gtctacatgt ccccgccatt ttgtttaacg ggtgatccac aacgtgggcc
     5701 aggcgtctgg cccatgccag ttatcgcagg tgggttctgc ggcgtaacgc accaggcgaa
     5761 aacgctgacc gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt
     5821 ttgctaaggt caccagttca cgcgattttt catcgaatgt tgcgttcatc agttccagtt
     5881 cattggtctc ctgaccgttg ttgaacggca aacgcaaccg taccgggcgc gaggcctgcg
     5941 gctttttacg cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc
     6001 taatcatcat cagcgcttta tcatcggtca gcgcagtgac attcacctca cgacgtaacg
     6061 gatcaagcga acagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttac
     6121 gttcttcgag tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag
     6181 gcgctggcgg tacgctgagc ggcggttcgt cccctttctt tatccacgcg gtctcactgc
     6241 caacgcgctt ttgctgggca tcaataaaca acaacgccgc ttttaagcca ctcagagaaa
     6301 tagtctgatt gccgtcgcgt aaggtgatcg ccctcccttc ctgaatcatc tgcaaaaacg
     6361 cggtgatggt tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac
     6421 cacttaacgc caacggctca ccatctaaca gcagccgtgg cgctatctcc ccttctgacg
     6481 catccggcga cttcaacccg ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg
     6541 cgctgcggct cagggtcatc accagtccat tatgatcgcc cgtattgcgc gctacacaga
     6601 aattttgatt attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc
     6661 acaccaaaga catcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tatcgcatgg
     6721 acggtacgac cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag
     6781 cgcggctcaa tcggatttgt cggattgagc caataataaa caacgaaata ttattgttat
     6841 ttttcagtcc attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc
     6901 acgtatctcg ccggttttaa tcatctccag cgcctggctg aacggcagct caagcacttc
     6961 aatatcttca tcttcgacac caccaccggc gttagcgcgc tggctgtcac tgtattcggc
     7021 gataaaaaag tggattagct cagtcacacc gcctggcgac atatacagtt caaataattt
     7081 gcggacttcg ccaacttcat agcccgtctc ttcaatcgct tctttgcgaa tgcacacttc
     7141 cggttcgtcg ttatccagca gcccggcgca ggtttcaatc agttgcccgc tttcattgcc
     7201 attaacccag gtagcgacgc ggaactggcg aatcagaacc acggtctttt tcttcgcgtt
     7261 gtacaggagg atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac
     7321 ttcgccatct ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga
     7381 gagaattttg tctttaacga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat
     7441 gaacaattat cttacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga
     7501 ctgattttac gcccagccag tcaatgatcc cctgtgccgc atgacgtcct tctgccattg
     7561 cggtaaccac cagatccgca ccacgcacgg cgtcaccacc ggcgaagatt ttcggattcg
     7621 tggtctggta acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg
     7681 ttacaccgtg cgactccagc cacggcatcc cgtgcggatt gaagccaaac gccataatca
     7741 ccgcatcggc tggcatgaca aattcactgc cttccaccgg cactggacgc cgacgtccct
     7801 gggcatccgg ctcgccaaga cgcgtgcgca ggaaacgaat cccgcagacg tgaccttgtt
     7861 cattcagctc aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcacggg
     7921 cgttcttcac ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg
     7981 tgacgttact cgcgccatgg cgcagcgcgg tacgcacaca gtccatcgcg gtgtcgccgc
     8041 cgcccagtac cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt
     8101 cgagtcccat cacctgttta gtgttggcga tgaggaacgg cagcgcgtca taaacgcccg
     8161 gcgcatcttc attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga
     8221 ccgcgtcgta ttgttccaaa agcgaattca aagagacatc tttacccact tcacaattga
     8281 gttcgaagtg aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat
     8341 ccagtttgaa agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt
     8401 acaccgtcac cgccacgcca ttgcgggtca gaacatctgc acaggccagc cctgccggac
     8461 ccgcaccgat aatcgccacc cgcttgtcca ctttggtgac atggcttaag tcaggacgcc
     8521 aacctttcgc caacgcctga tctgaaatgt agcgttcaat gttaccgata gttactgcgc
     8581 cgtgctcatc gcgaatagta caggcacctt cacacaaacg gtcttgcggg caaacgcgtc
     8641 cggtaatttc cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc
     8701 cggctttcac cagttcaatc cactgcggta tatggttatg cagcgggcag gtccattcac
     8761 aaacgctgtg ctcgccgcac ttaaggcagc gcgaggcttc ccgttgtgcc tgatcggcac
     8821 ggaatggcag ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatctg
     8881 gctcgccacg cgcgggcgtt gcctgcattt gctcgacttt actcattacc ggcatttctt
     8941 gcgccgcggt actggcatgc cacggttgat gttcctgacg tgcggtgcgc aagcggcggg
     9001 atttcgccat gccggagagt gcggcgtcag tgaccagttg cagcgcgtcc gccgggcaat
     9061 tttcgacaca ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg
     9121 cttttacttt tcctgccgca acgggtgtca gaacgatttg catcgtacca aatggacagg
     9181 ccaccacgca ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa
     9241 cgtggctgat tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac
     9301 aggtcacggc actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt
     9361 gttggctcag gacatgttgc tcgtcattgt gagccatgac acaggcgatt tcacaagcat
     9421 gacaacccag acattgctga ctgttggcca taataaaacg attcataacg accttctttt
     9481 ttggttgtaa aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaat
     9541 cggcaatgtt catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa
     9601 taatcccatc actgactaaa ttgcgtttca gcgaactgga agattaatga ttttttgtgg
     9661 agaagacgcg tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt
     9721 acattgtgct gctgtcgatt ctttccacgg gtatcgctct gctaacgctg gcgagcagtt
     9781 tacgcgacgc tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg
     9841 gctacgactt gcaaagtggc agtccacaac tcaatgcaca tcgccagtta tttcagcagg
     9901 cactgcattc accggtatta accaatctca acgtctggta tgtgccagag gcagtaaaaa
     9961 cccgctatgc gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg
    10021 acttgccgtg gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc
    10081 tggctttaca gcactacgct gaacgcaaaa tgctgctggt ggtggcaatt tccctggctg
    10141 gcggcatcgg tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg
    10201 ttgccccgct gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact
    10261 cgccgccgct ggataccagc ctgccgaatg agcttggcct gcttgcaaaa acctttaacc
    10321 agatgtcgag cgagctgcat aaattgtacc gttcgctgga ggcgtcagta gaagaaaaga
    10381 cccgcgatct ccacgaggcc aagcgtcgtc tggaggtgtt gtatcagtgt tcgcaggcgc
    10441 taaacaccag ccagattgat gtgcattgtt tccgccatat tttgcagatt gttcgcgaca
    10501 atgaagcggc tgaatatctg gagttaaatg tcggtgacaa ctggcggatt agcgaagggc
    10561 agccaaaccc ggaattgccg atgcagattt taccggtgac aatgcaagag acggtttacg
    10621 gagaactgca ctggcaaaat agtcacgttt catcatcaga accgctgctt aacagcgttt
    10681 cgtcgatgct gggacgcggt ttgtacttta atcaggcgca gaagcatttt cagcagttat
    10741 tgttgatgga agaacgtgcg accatcgccc gcgaattgca cgactcgctg gctcaggtac
    10801 tttcttactt acgtatccag ttgacgttac tgaagcgttc gataccggaa gataacgcca
    10861 ccgcacaaag tatcatggcc gatttttccc aggcgttgaa tgatgcttat cggcagttac
    10921 gcgagctgtt gactactttc cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga
    10981 gggaaatgct ggatacgtta caaaatcaaa ccagcgccaa actgaccctc gactgccgtc
    11041 tgccaaccct ggcgctggat gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag
    11101 cggtgctgaa tgcgatgaag cacgccaacg ccagcgaaat cgccgttagc tgcgtcaccg
    11161 cgccggatgg caatcacaca gtctatattc gcgacaacgg gattggcatc ggtgaaccga
    11221 aagaacccga aggccattat ggtctgaata tcatgcgcga acgcgcggaa agattgggtg
    11281 ggacgctgac tttttcacaa ccttccggcg gcggcacgtt agtgagtatt agctttcgct
    11341 ctgcggaggg tgaggaaagt cagctgatgt aatgcctctt attgaccaaa gaatagtggc
    11401 acttaaggtt cagtataaaa gggcatgata atttacatta actccttttt ttctccacga
    11461 ttggctcgta ccttgccgct acagtgaagc aagccaagcc tacaacgata cgcagaaaca
    11521 cgaggtcctc ttttaatggc gaatttcttt attgatcgcc ccatttttgc ctgggtgctg
    11581 gcaatcctgt tgtgtctgac aggtaccctg gcgatttttt cattgcccgt tgaacaatac
    11641 cccgatctcg cgccaccgaa tgtgcgagtg accgctaact atcccggcgc atcggcccag
    11701 acgctggaaa acaccgtgac ccaggttatc gagcaaaata tgaccggcct cgataatctg
    11761 atgtatatgt catctcagag cagcggcacc ggtcaggcat ctgtcacgtt aagttttaaa
    11821 gcaggcaccg atccggacga agccgtgcag caagtacaaa accagctgca atcagccatg
    11881 cgaaagttac cgcaggcggt gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc
    11941 aacattctga ccattgcctt cgtctctacc gatggttcga tggataaaca ggatattgcc
    12001 gattatgttg ccagtaatat tcaggacccg ttaagccgcg tgaatggcgt cggggatatc
    12061 gatgcctatg gttcgcaata ttccatgcgt atctggctgg acccggcgaa actcaacagt
    12121 ttccagatga cggctaaaga tgtcaccgat gccattgaat cacagaacgc gcagattgcg
    12181 gttgggcaac ttggtggtac gccttccgtc gataagcagg cgctcaacgc caccattaac
    12241 gcccagtcac tgctgcaaac accagaacag ttccgcgata tcaccttgcg ggtgaatcag
    12301 gacggctcag aggtaaggct gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac
    12361 gattatctta gccgcttcaa tggtaagcca gcctccgggc tgggggtaaa actggcctcc
    12421 ggcgcgaacg aaatggcgac agcggagctg gtgctcaatc gtctcgacga gctggcgcag
    12481 tattttccac acggactgga atacaaggtg gcgtatgaaa ccacctcttt tgtaaaagcc
    12541 tccattgaag acgtggtgaa aacgcttctg gaagctatcg ccctggtttt cctcgttatg
    12601 tatctgttcc tgcaaaactt ccgcgccacg ctgataccca ctatcgccgt gccggtggtg
    12661 ttgatgggaa ccttctcggt gctttatgcc ttcggctata gcgtcaacac cttaaccatg
    12721 ttcgcgatgg tgctggcgat cggtctgctg gtggatgatg ccatcgtggt ggtggaaaac
    12781 gtcgaacgta ttatgagtga ggaaggactc actcctcgcg aagccacacg taaatcgatg
    12841 gggcagatcc agggggcact ggtcgggatt gcgatggtgc tttcggcggt atttgtgcca
    12901 atggccttct tcggcggcac caccggtgcc atctatcgcc agttctctat taccattgtt
    12961 gcggcgatgg tgctgtcagt actggtagcg atgatcctca ctccggctct gtgtgccaca
    13021 ctacttaagc cactgaaaaa aggtgagcat cacgggcaaa aaggcttttt tgcctggttt
    13081 aaccagatgt ttaaccgcaa cgccgaacgc tacgaaaaag gggtggcgaa aattctccac
    13141 cgtagcctgc gctggattgt gatttatgtc ctgctgcttg gcggcatggt gttcctgttc
    13201 ctgcgtttgc cgacgtcgtt cttaccgctg gaagaccgtg gcatgtttac tacctcggta
    13261 cagttgccca gcggttcaac ccagcaacag accctgaaag tcgttgagca aatcgagaaa
    13321 tattacttca cccatgaaaa agacaacatc atgtcggtgt ttgccaccgt gggttctggc
    13381 cctgggggga acgggcaaaa cgtggcgcga atgtttatcc gcctgaaaga ctggagcgaa
    13441 cgcgacagta agaccggcac ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac
    13501 aagattaaag aagctcgcgt tatcgccagc agcccgccag caattagcgg tcttggtagt
    13561 tctgcgggtt ttgatatgga gttgcaggac cacgctggag cgggtcacga tgcgctgatg
    13621 gcagcacgaa accagttgct ggcgctggca gcggaaaacc cggagctaac ccgtgtgcgc
    13681 cataacggcc tcgacgacag tccgcagttg cagattgata tcgaccagcg taaagctcag
    13741 gcgctgggcg ttgctatcga cgatattaac gacacactgc aaaccgcctg gggttcgagc
    13801 tatgtgaatg actttatgga tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg
    13861 ccgtatcgca tgctgccaga tgacatcaat ctctggtatg tccgaaataa agatggcggc
    13921 atggtgccct tctctgcttt cgcgacctca cgctgggaga caggctcgcc gcgtctggaa
    13981 cgctataacg gttattctgc ggttgagatt gttggggaag ccgcaccggg ggtcagtacc
    14041 ggtacggcga tggatattat ggaatcgtta gtgaagcagt tgccaaacgg ctttggtctg
    14101 gagtggacgg cgatgtcgta tcaggagcgg ctttccggcg cgcaggctcc ggcgctgtac
    14161 gccatttcct tgctggtggt attcctgtgt ctggctgcgc tgtatgaaag ctggtcggtg
    14221 ccgttctcgg taatgctggt cgtgccgctg ggggttatcg gcgcgctgct ggcaacctgg
    14281 atgcgcgggc tggaaaacga tgtttacttc caggtgggcc tgttaacggt cattggttta
    14341 tcggcgaaaa acgccatcct gatcgtcgag tttgctaacg agatgaacca aaaaggccac
    14401 gacctgtttg aagcgacgct ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc
    14461 tcgctggcat ttatcttcgg cgtattgcca atggcaacca gcacaggtgc cggttccggt
    14521 ggtcagcatg cggtgggtac tggcgtaatg ggcgggatga tttcggccac tattctggct
    14581 atttacttcg tgccgctgtt ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc
    14641 ccggaataag caataaaaaa ggcgacatgc caatgtgtcg cccttttcaa ctttccgatt
    14701 aagaacctgc tcagcgggtt cttgctgttt gtactttgtc tcaggaatta cttacgaagc
    14761 ataacttcga taaagtcttt ccagttcccc agttcacgtt caatcataac aacctctctt
    14821 ataattatgg gtattctacg gaaacaatat accgtggtga agctaattta ctcgattgct
    14881 gcgatgacta cctccggggg acaaacctta tgtaaatact atggtcctgc ggtgatgatt
    14941 tgtatgtgat acaccgcaac atttcgagat attcatacgg catcta
//
LOCUS       DAJOTB010000118        14907 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJOTB010000118 DAJOTB010000000
VERSION     DAJOTB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.118"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(191..841)
                     /locus_tag="OHN28_003551"
     CDS             complement(191..841)
                     /locus_tag="OHN28_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298876.1"
                     /translation="MNNILRKSPIERLCSSVSITPHEMAVALAGLNPSMRISDVPEEN
                     FEYVDFVRTHLARAIKVYRGEKTSKDEPCHALDIFLASYPFIDTNTPEIIVQKISEAI
                     DDLRGTKGWEEKARNLGGLQLVNYIKETNRSGRGQHRKQDEENGTMKMMGLIVHLLIK
                     KSGSSSYIQNGKPNRTAIYRDIETLIKDEGISHKGIAKATFLEKISRALLAVHDLD"
     gene            complement(838..2106)
                     /locus_tag="OHN28_003552"
     CDS             complement(838..2106)
                     /locus_tag="OHN28_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2298877.1"
                     /translation="MPKLTDMQIRAWIKAGDRFDGKADGNGLYICYPKNYTVPFWRFR
                     YKLAGKQRAMVIGSYSELSLSKARETAKELSARVALGYDVAAEKQVRKAEALAKMEAE
                     KNAIRVSDLAAEYFERQILPRWKHPDILRRRIDKDINPCIGHMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVSDAGGKEVSRDRWLTRD
                     ELIRLFEAMRTAKGFSRQNELTFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPFDKARRQM"
     gene            complement(2295..3158)
                     /locus_tag="OHN28_003553"
     CDS             complement(2295..3158)
                     /locus_tag="OHN28_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCQ2298878.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            3285..4001
                     /gene="rph"
                     /locus_tag="OHN28_003554"
     CDS             3285..4001
                     /gene="rph"
                     /locus_tag="OHN28_003554"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCQ2298879.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4067..4708
                     /gene="pyrE"
                     /locus_tag="OHN28_003555"
     CDS             4067..4708
                     /gene="pyrE"
                     /locus_tag="OHN28_003555"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCQ2298880.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(4745..5341)
                     /gene="slmA"
                     /locus_tag="OHN28_003556"
     CDS             complement(4745..5341)
                     /gene="slmA"
                     /locus_tag="OHN28_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCQ2298881.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(5448..5906)
                     /gene="dut"
                     /locus_tag="OHN28_003557"
     CDS             complement(5448..5906)
                     /gene="dut"
                     /locus_tag="OHN28_003557"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCQ2298882.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(5884..7104)
                     /gene="coaBC"
                     /locus_tag="OHN28_003558"
     CDS             complement(5884..7104)
                     /gene="coaBC"
                     /locus_tag="OHN28_003558"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCQ2298883.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            7276..7944
                     /gene="radC"
                     /locus_tag="OHN28_003559"
     CDS             7276..7944
                     /gene="radC"
                     /locus_tag="OHN28_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCQ2298884.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8161..8397
                     /gene="rpmB"
                     /locus_tag="OHN28_003560"
     CDS             8161..8397
                     /gene="rpmB"
                     /locus_tag="OHN28_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCQ2298885.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            8418..8585
                     /gene="rpmG"
                     /locus_tag="OHN28_003561"
     CDS             8418..8585
                     /gene="rpmG"
                     /locus_tag="OHN28_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCQ2298886.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            8683..9492
                     /gene="mutM"
                     /locus_tag="OHN28_003562"
     CDS             8683..9492
                     /gene="mutM"
                     /locus_tag="OHN28_003562"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCQ2298887.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(9531..10010)
                     /gene="coaD"
                     /locus_tag="OHN28_003563"
     CDS             complement(9531..10010)
                     /gene="coaD"
                     /locus_tag="OHN28_003563"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCQ2298888.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(10018..11295)
                     /gene="waaA"
                     /locus_tag="OHN28_003564"
     CDS             complement(10018..11295)
                     /gene="waaA"
                     /locus_tag="OHN28_003564"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCQ2298889.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            11709..12767
                     /gene="rfaQ"
                     /locus_tag="OHN28_003565"
     CDS             11709..12767
                     /gene="rfaQ"
                     /locus_tag="OHN28_003565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCQ2298890.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            12764..13888
                     /locus_tag="OHN28_003566"
     CDS             12764..13888
                     /locus_tag="OHN28_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2298891.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            13881..14678
                     /gene="rfaP"
                     /locus_tag="OHN28_003567"
     CDS             13881..14678
                     /gene="rfaP"
                     /locus_tag="OHN28_003567"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCQ2298892.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLLNMASVKAEHIKERTQRKGL"
     gene            14715..>14907
                     /locus_tag="OHN28_003568"
     CDS             14715..>14907
                     /locus_tag="OHN28_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCQ2298893.1"
                     /translation="MTIYFINWVADYELKMIQYLKKKHKIKNITTPKKYNWVNKKISK
                     LGMDNAWLGRLFIKHHLNAV"
BASE COUNT         3681 a         3565 c         3845 g         3816 t
ORIGIN      
        1 gtcggtttat aatggctcat gatggcttaa aatggctaat gatagctggc gtatctttta
       61 gagatggggt gattacagat gaactaagcc gaacagatat agcaatggct aggcatagaa
      121 ttatctatgc tggtggagat gggtaaacag gtaatgcccc gccattgctt tggaggggcg
      181 tgaaaagtga ttagtcgaga tcgtggacag ctaataaagc ccgtgagatt ttttctagga
      241 atgtggcttt tgctattcct ttatgggata tcccttcatc cttgattaag gtttctatgt
      301 cacggtatat agctgtgcga ttaggtttcc cattttggat gtaggaagat gagcctgatt
      361 tttttatcag cagatgtact atcaacccca tcattttcat tgtcccgttt tcctcatcct
      421 gctttctatg ctgcccacgt ccgctgcggt tggtttcttt gatgtagttt actaactgga
      481 gtcctccaag gtttcgggct ttttcttccc agcccttggt gccacgcaaa tcatcaatcg
      541 cttctgatat tttctgaaca attatttctg gtgtatttgt gtcaataaat gggtaagaag
      601 ctaagaaaat atcaagggca tggcaaggct cgtctttact agttttttca cctctgtaca
      661 ccttgatagc tctggcaaga tgagttctga caaaatcgac atattcgaaa ttttcctcag
      721 gcacatcgct aatgcgcata gatgggttta ggccagccaa ggccacagcc atttcatgag
      781 gggtaatcga tacagatgaa caaagccgtt cgattggcga ttttctcaag atgttgttca
      841 catttgcctc cgggctttat cgaatggcgt tacgttgtag tctttcccgc tttccagtgc
      901 caccagcaga tcagcccact gtgccagcgc agctttacgc tcatcaaaat actgatggcg
      961 gttataaatc ccctccacac ccttaatgcg atgattgagg catcgttccg ctacgacagg
     1021 atcaacaccc aatgctgcta aatgagtacg tgcggtacgc cggaagtcgt gaatcgtgaa
     1081 attaggcaca tccggcattt cggcgcgaac cttagccagt gctacgggta aagtgctttc
     1141 ctgaatatgt gggatcattc tgttttgcat cttcctggct ggaagcaccc atgcgctatt
     1201 acatgaaaag gtgtgtagct ctctcaacca ttcaacggct ggggaaggta gaggtatatc
     1261 aatagggtct ccgtttttgc tgcgttcttc cggcaaatgc cataccgcac catctaaatc
     1321 aaactcttcc catcgtgcgg cgcataactc catcttacga acgcatagtg ccagcaatag
     1381 cttaaatgtc agttcgttct ggcggctgaa tccttttgcg gtgcgcatag cctcgaatag
     1441 cctgataagc tcatcgcgag ttagccaacg gtcgcgggaa acctctttac caccagcatc
     1501 agatacttca aacgctgaac aagggtttat ctccagagcg tggcgtttta tcccatagtc
     1561 aaagatacgg cgtgtccagc gtaatacatc tgttgctatc gttggtgctc ctcggtcaac
     1621 gatacctttc aacatgtcgt caatgtggcg tggcttaacg tcctctacct tcatatgccc
     1681 aatgcagggg ttaatatcct tatcaattct ccggcgaagg atatcgggat gtttccatct
     1741 cggaagtatc tggcgctcga agtattcggc ggccagatct gaaacgcgta tagcgttttt
     1801 ctcagcctcc atttttgcca gtgcttcagc ttttcgtacc tgtttctctg ctgctacgtc
     1861 atagccaaga gcaactctgg ctgacagctc ttttgctgtt tctctggctt ttgatagtga
     1921 cagttcggag taagagccta taaccatagc ccgctgcttt cctgcgagct tatagcggaa
     1981 tcgccagaac gggactgtgt agtttttggg gtagcaaata taaaggccgt tgccgtctgc
     2041 cttgccatca aatctgtctc ccgcctttat ccatgcgcgg atctgcatat ctgtgagttt
     2101 tggcattgcg aatacctgaa tatcgaatat gggggtgtac ataaagcaat gtacatgaaa
     2161 gtcattatgt acacctctat gtacacgcat attgtgcgct ttgttgcgtc gttatgatag
     2221 ctcatgataa caaaaaagcc cgacatttcg cgggctttgt ggttttttgc tgcctggtga
     2281 tttttactga tagcttattc gatattctga atctgctcgc gcatctgttc aatcaacact
     2341 ttcagctcga tggcggagtt tgtcacttcg gcgttgatag atttcgacgc aagagtgttc
     2401 gactcgcggt tgaactcctg catcataaag tccagacgac gaccaacggc ttcttttttc
     2461 ttcagaatgt tgtaggtctc tttgacatgc gcttcgaggc ggtccagttc ttcggcaacg
     2521 tcaattcgtt gtgccagcag aaccaactcc tgctccagac ggttgttttc cagttgcacc
     2581 tgagcatctt ccagcttcgc taccagacgc tcacgctgcc attgcaggat ttccggcata
     2641 tgggcgcgga ctttgaccac ttcggcggtg acgccttcca gacgctgctc aatcaacact
     2701 ttcagcgcct gaccttcggt ttcgcgcgcg atgataaagt catccagggt gccatccaac
     2761 gccgcgagaa tttcagctgc aatcgcgtcg agatcttgct cctgggctgc catcacgccc
     2821 ggccagcgta gaatatcaac cgggttgatt tctccttcgt cgctctgcat ttttacccag
     2881 ttcgcggcag ttaccagctg tttagccagt ttttcgttga ggatcagctc accttgcgcg
     2941 ctaacatctg gctcatagcg cagagtacat tccactttac cgcgcgtcag gcgagaacga
     3001 atacgctcgc gaacgacagg ttcaaggcta cggaactgct ccggcagacg aaagtaagtt
     3061 tccagataac gctggtttac cgagcgcatt tcccaggttg cgctccccca ttcacccttg
     3121 atttcacgcc gggcgtaggc ggtcatactg cggatcatag acgttcctgt ttataaaagg
     3181 agaggtggaa ggattatagc catcgatgcc ttgtcaggat aggaataacc gccggaagtc
     3241 cgtataatgc gcagccacat ttgtttcaag ccggagattt caatatgcgt ccagcaggcc
     3301 gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa catgcagaag
     3361 gctcggtgct ggtcgaattt ggcgatacca aagtattgtg taccgcctct attgaagaag
     3421 gcgtgccgcg cttcctgaaa ggtcagggcc agggctggat caccgcagag tacggtatgc
     3481 tgccgcgttc tacccatacc cgtaacgcgc gtgaagcggc gaaaggtaag cagggtggac
     3541 gcacaatgga aatccagcgt ctaatcgccc gtgctcttcg cgcggcagta gatttgaaag
     3601 cactgggtga gttcaccatt acgctggact gtgacgtgct tcaggctgat ggtggcacgc
     3661 gtaccgcttc gattacgggt gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg
     3721 aaaacggcaa gctgaaaact aatccaatga aagggatggt agccgcggtc tctgtcggga
     3781 ttgtgaacgg tgaagcggtt tgcgatctgg aatatgttga agactctgcc gcagagaccg
     3841 acatgaacgt agtgatgacc gaagacgggc gtatcattga agtgcagggg acggcagaag
     3901 gcgaaccgtt cacccacgaa gagttactca ccttgttggc gctggcccga gggggaatcg
     3961 aatccattgt agcgacgcag aaggcggcgc tggcaaactg atttttaagg cgactgatga
     4021 gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga aaccatatca
     4081 gcgccagttt attgaatttg cgcttagcaa gcaggtgtta aagtttggcg agtttacgct
     4141 gaaatccggg cgcaaaagcc cctatttctt caacgccggg ctgtttaata ccgggcgcga
     4201 tctggcactg ttaggccgtt tttacgctga agcattggtg gattccggca ttgagttcga
     4261 tctgctgttt ggccctgctt acaaagggat cccgattgcc accactaccg ccgtggcgct
     4321 ggcggagcat catgaccttg acctaccgta ctgctttaac cgcaaagagg caaaagacca
     4381 cggtgaaggc ggtaatctgg ttggtagcgc gttacaagga cgcgtaatgc tggtagatga
     4441 tgtgatcacc gccggaacgg cgattcgcga gtcgatggaa attattcagg ccaatggcgc
     4501 gacgcttgca ggcgtgttga tttcgcttga tcgtcaggaa cgcgggcgcg gcgaaatttc
     4561 ggcgattcag gaagttgagc gtgattacaa ctgcaaggtg atctctatca tcaccctgaa
     4621 agacctgatt gcttacctgg aagagaagcc agaaatggcg gaacatctgg cggcggttaa
     4681 ggcctatcgc gaagagtttg gcgtttaacg aaactcgccg gatgaaaagt catccggcgt
     4741 catattactg caactgtgcg gcaattagcg gccagcgggc gtcaaaatca tccgtcgggc
     4801 ggtatttaaa ctcgctgcgg acaaatcgtg acagcatacc ttcacaaaat gccagcaact
     4861 ggcttgccag cagggtttca tcggtggtgt aaccttcacc ctcacgcatt ctcttttcac
     4921 gcaatacctg acgcaactgc gcttcaatac gctcgaacag ctggttgatg cgcccttgca
     4981 gacgatcctg ttcgaacatt agcgcatgac cagtgaggat gcgagtcagg ccaggattac
     5041 gctcaccaaa accgaggagc aacaacacga tcagacgcag gcgcgctgtg gtgtctttct
     5101 catctttcag aatcaggttg atacgagtaa tcaggctatc ttcgataaac tcaatcaggc
     5161 tatcgaacat gcgggtctta ctggggaagt ggcgatacag tgccgcttcg gaaacgccga
     5221 cagaggcggc cagttttgcc gtcgtgatac gttggcttcc atcgctggat tccagcatca
     5281 gcgccagaga ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt ttttctgcca
     5341 tgttacaaaa tacccctgaa aataagcact tgccaggcgg cacccacgct atgaccgcaa
     5401 acgaaatgtt tgcggctatg ttatgacgtt attcggatgc gtatgtgtta ctgacgacca
     5461 gagtgaccaa agccgccttc accgcggtcg gtggcgtcga aatcttccac cagattaaat
     5521 tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg ttgaatggtg
     5581 aagctgtcct gaccacggtt ccacacggaa atcatcaact ggccctgata gtcagaatcg
     5641 atcaatccta ccaggttacc aagcacgata ccgtgcttat gtcccaatcc ggagcgcggt
     5701 agcatcattg ccgccagtga aggatcggca atatgaatcg ccagcccggt cggaaccagc
     5761 gtagtgtcac ccggagccag ttctacggcg tcgtcgagac aggcacgcag gtcaagtccg
     5821 gcagagccag aggtggcata agtcgggagc ggaaattcct tcccaacgcg cgggtccaga
     5881 atcttaacgt cgattttttt catcataacg ggtcacgatc tcgtcgagta ataattggcc
     5941 aaggagctct ttgcgctcaa gcggtaagac tttatctccg tcctgccaga aaaggtgtaa
     6001 tgcgttgttg tcgctgttaa atccttgagt tggctgggaa acatcgttcg cgcagatcag
     6061 atcaaggttt ttacggatac gtttttgccg ggcgtattct tccacattat ttgtttcggc
     6121 ggcaaatcca acgacgtaag gtcgatggtc ttttagtgcg gcaacgcctg cgacgatatc
     6181 ggggttttta accattttta ttgttaattc atcaccctgc gtggcctgct ttttgatttt
     6241 ttctggggcc acggtagctg cgcgataatc cgccacggcg gcgcaaccga taaaaatatt
     6301 ttgctgctgt actgaagcat tcacggcggc ttccatttcc agcgcggtca tcacatcaac
     6361 acggttaaca aacggtggcg tcggtagtga aaccggacct gataccagcg tgacgttcgc
     6421 gccacgacgg gctgcggcgg cggcgatagc aaaacccatc ttgccggagc tgtgattaga
     6481 gatataacgc acgggatcga gcggttcacg cgtcggaccg gcggtaatca taatgttcag
     6541 atgtttcagg tcgttgacgg gcgaaaaatg cgctaccgcc atatccacaa tagttaacgg
     6601 atcgagcatt cgtcctgggc cgatatcacc acaagcctga ctgccgctgt ctggccccca
     6661 gatgagcaaa ccacgtgagg caagcacctc taaattatgc tgggtggcgg cggcacggta
     6721 catctgctgg ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca gacaaatcgt
     6781 cgataccagg tcattcgcca ttccggctgc aacacgggca attaaatctg ccgtggcagg
     6841 ggcgagaatc actaaatcag cccattttcc cagctcaata tggcccatag cggcttcggc
     6901 tgccgggtcc agcagactgt cggaaacggg ataaccagaa accgcctgca agctaagtgg
     6961 ggtgataaag gcttttgccg cttcggtcat ggctacgcag acatcggccc cgcgatcgcg
     7021 caaacgacgc accagttcag gggttttata ggcagcaata ccgccgctaa cgccgagaac
     7081 gattttttta ccggccaggc tcatgatgat ttttcctgtt gggtgacacc agaagttggc
     7141 gattttatca caatactttt gttgtcgtgc cttcacctga gattcacttt gcgaggcgct
     7201 ttccaggatt gaaaactggc cgtcgattta aaggaacggc tatgacagga tgcgagcacc
     7261 acaaaggagg tgaaggtgaa aaacaatgta cagctgttga tgccgcgcga aaaaatgctg
     7321 aagtttggta ttagcgcctt aacggatgtc gagctgctgg cgctatttct gcgtaccgga
     7381 acgcgcggta aagatgtatt aaccctggca aaagagatgc tggagaattt cggctctctt
     7441 tatggcttgt taacctctga atatgagcaa tttagtggcg ttcatggaat tggcgtggcg
     7501 aaattcgccc agttaaaggg gattgccgaa ctggcgcggc gttactacaa cgtacggatg
     7561 cgtgaagaaa gccctttact cagcccggag atgacgaggg aatttttaca aagccagctc
     7621 acgggcgagg agcgggagat ctttatggtg atctttctcg actcccaaca cagggttata
     7681 acgcatagcc gtcttttttc cggcacgcta aaccatgttg aagtccatcc tcgggaaatt
     7741 atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc ttgcgcataa tcacccttcg
     7801 ggttgtgctg aacccagtaa agcggataaa ctcattactg aacggataat aaagagttgt
     7861 cagttcatgg atttacgcgt gctcgaccat atcgtgattg ggcgtggaga gtatgtttct
     7921 tttgccgaac gcggctggat ttaacccgct atgcgcgatc cttcgggatc tttgtctgtt
     7981 cgggacttga gcacatcgct gagtcagcgt atactacgcc acctttgaga atctcgggtt
     8041 tggcatttgg gcctggcaat cgagagttca catagaactg cgatgaccgg gctgtaaagc
     8101 ctgacgaggc gccaataccc catacgaagc tcgagctaat ttgatttttg gagaatagac
     8161 atgtcccgag tctgccaagt tactggcaag cgtccggtga ccggtaacaa ccgttcccac
     8221 gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc actctcaccg tttctgggtt
     8281 gagagcgaga agcgttttgt caccctgcgc gtatctgcta aaggtatgcg tgtaatcgat
     8341 aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc gtggcgaaaa gtactaagta
     8401 cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg gtttcttctg
     8461 ctggtactgg tcacttctat accactacga agaacaaacg tactaagccg gaaaaactgg
     8521 aactgaaaaa attcgatcca gttgttcgcc agcacgtgat ctacaaagaa gcgaaaatca
     8581 aataatttcc gctttgatgt aacaaaaaac ctcgccccgg cggggttttt tgttatctgc
     8641 ttgcccccat attgactgca tctgttcatt cctggagatg ctatgcctga attacccgaa
     8701 gttgaaacca gccgtcgcgg catagaaccg catctcgttg gtgcaaccat tcttcatgcg
     8761 gtggtgcgca acggacgctt gcgctggccg gtttcagaag agatctaccg tttaagcgac
     8821 caaccagtgc ttagcgtgca gcgccgggct aaatatctgc tgctggagct gcctgagggc
     8881 tggattatca ttcatttggg gatgtctggc agcctgcgca tccttccaga agaacttccc
     8941 cctgaaaagc atgaccatgt ggatttggtg atgagcaacg gcaaagtgct gcgctacacc
     9001 gatccgcgcc gctttggtgc ctggctatgg accaaagagc tggaagggca caacgtgctg
     9061 gcccatcttg gaccggagcc gcttagtgac gatttcaacg gtgagtacct gcatcagaag
     9121 tgcgcgaaga aaaaaacggc gattaaaccg tggctgatgg ataacaagct ggtggtaggc
     9181 gtagggaata tttatgccag cgaatcgctg tttgcggcgg ggatccatcc ggatcggctg
     9241 gcgtcatcac tgtcgctggc ggagtgtgaa ttgttagctc gggtgattaa agcggtgttg
     9301 ctgcgttcga ttgagcaggg cggtacaacg ctgaaagatt ttctgcaaag tgatggtaaa
     9361 ccgggctatt tcgctcagga attgcaggtt tacgggcgaa aaggtgagcc gtgtcgggtg
     9421 tgcggtacgc cgattgtggc gactaaacat gcgcagcggg caacgtttta ttgtcggcag
     9481 tgccagaagt aattgatgcg cgccggatgg cataccatcc ggcataaact ctacgctaac
     9541 ttcgccatca gcgcctgatg gacattctcc ggcaggaaat gggtgacatc gccctgatgg
     9601 cgcgccacct ctttcaccaa cgatgaagag ataaacgacc actctttcga cggcatcaga
     9661 aacacacttt ccagctccgg cattaagtgg cgattcatat gcgccagctg catttcatat
     9721 tcaaaatccg ccaccgcacg caggccacga atcagcaccg tagcgtgttg attacgggcg
     9781 aagttcgcca tcaaatcact aaacccgacc acttccacgt tctccagatg cgcggttgcc
     9841 tgctgtgcca gctccacacg ctcttccagg gtaaacatcg gttttttact gggactagcg
     9901 gcaatcgcca gaataacgtg atcgaacatt tgcgaggcgc gcgtcacgat atcgatatga
     9961 ccattggtaa tgggatcgaa agtacccgga taaatcgccc gtttttgcat aacaacctca
    10021 atgcgttttc ggtggcaggt aaggttccag cagttgaagc agacgctgta gtgcgccctg
    10081 gttttgatac agcacttcaa cggcatgacg gccatagaaa ctacggtaat cggcgtcggt
    10141 gagtaaagag gaaacctctt ttgcaagcgt agtggcatcg gtaacggtaa tcagcccgct
    10201 tgcctgctcc agccgcgcgc aaatgtcttt aaagttaaac gtatgcggcc ccatcaatac
    10261 cggaatagcg tgtgcggccg cttccagcgg attatgccca ccacgttcaa ccagtgaacc
    10321 gccaacaaag gcgagatcgg caatgccgta cagtaacatc aactcgccca tcgtatcgcc
    10381 aaccacaacc tgcgtgctgg tggaggggac ttcccctgaa gatcgtgtga tatagcttag
    10441 tccagcctgg cggacaaggt taatcgcatc cgggaagcgt tctggatgac ggggtaccag
    10501 gatgagcaat aaattcggga attgctgtaa caatgcctga tgtgcggcga tcaccacact
    10561 ctcttcgcct tcatgagtgc tggtggcaat ccataccggg cggtgtggtg cccactggcg
    10621 gcgcagcgtc acggctttag cagccaactg cggcgttaca gaaatatcga atttcaggct
    10681 accggtaacg gtcacctgat tattttttgc gcccagcgcc acaaaacgtg caccatcttc
    10741 ttcattttgg gcggcaatca gcgtaatacg acgcagcaag cggcggacga atttacccag
    10801 tttggcataa cctgcggccg agcgggcaga gagtcgcgcg ttagcgatca ccagcggaat
    10861 tttacgttta tgtagcgccg caatcaggtt aggccatagt tcggtttcca taatcaacac
    10921 cagtttaggg tcgactttat tcaggaaacg gttgagtgca tcgggcaggt catacggcag
    10981 ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg cgctccgaac cggttggagt
    11041 catggttgtt acggtaatcg gtaaatcagg ataacgatga cgcagtgcgc gcaccaacgg
    11101 gatcgccgcc agagtttcac cgacggagac ggagtgcagc ataatgccgc ctggttttag
    11161 cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt cgataggccg gagccttacg
    11221 tccgcgcacc cagagccgta tccagatcag tggctgaata aggtagagaa gggcggtgta
    11281 aagcaattcg agcatagtaa atagctgact tatggatgtg ctggggattc tatgtattta
    11341 gctgtggctt taccattact tttcccgttt ttgacttaaa tagcaattta cccgccaatt
    11401 cttgctgaac atctatactt tttttgtatt tttatgcgat tcattgattt ctgcagtacg
    11461 tgcaggaagg aatcgcagag caacagtaaa aagaatatgg ttgctaatga ttttatttga
    11521 ttgatttctt tattgaaaat caacaatgtg cttaaaaatc attcgggtcg gattgccgcg
    11581 aaagaagtga tacactagcg cgtcaatatc agtgcggtat cagtattcag gtagctgttg
    11641 agcctggggc ggtagcgtgc tttttctgct tcaccttaat cggataatct caacaaaaag
    11701 agttacttgt ggataagcca tttcgaagaa ttttgctcat taagatgcgt tttcatgggg
    11761 atatgttatt aactactccc gtcattagtt cgctgaaaaa aaattaccct gacgcaaaaa
    11821 tcgatgtgct gctttatcag gacaccatcc cgatcctgtc tgaaaatcca gagattaacg
    11881 cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc aacttttttc
    11941 atctcatcaa ggtattacgt gccaataagt atgaccttat cgttaatctt accgatcaat
    12001 ggatggttgc tatactggtt cgcttattaa atgcccgtgt gaaaatttcc caggattatc
    12061 atcatcggca gtctgctttt tggcgtaata gtttcactca tttggtgccg ttgcagggtg
    12121 gaaatgtggt ggaaagtaac ttatccgtgc tgacgccatt gggacttgaa tcgttggtga
    12181 agcagacaac catgagttac ccgcctgcaa actggaaacg tatgcgtcgc gaacttgatc
    12241 acgctggtgt tggacaaaat tatgtggtta tccaacctac ggcgcggcaa atcttcaaat
    12301 gctgggacaa cgccaagttt tccgctgtga ttgatgcctt acatgctcgt ggttatgaag
    12361 tcgttctgac gtccggccca gataaagacg atctggcctg cgtcaatgaa attgcgcagg
    12421 gatgccagac gccaccagta acggcgctgg ccggaaaggt gaccttcccg gaacttggtg
    12481 cgttaatcga tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg catattgccg
    12541 ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac ggaccatatt ttctggcgtc
    12601 cctggtcaaa taacatgatt caattctggg cgggagatta ccgggaaatg ccaacgcgcg
    12661 atcagcgtga ccgaaatgag atgtatcttt cggttattcc ggcggcagat gtcattgcag
    12721 ctgtcgataa attacttccc tcctccacga caggtacgtc gttatgatcg ttgctttttg
    12781 tttatataaa tattttccct ttggcggttt gcagcgcgat tttatgcgta ttgctcagac
    12841 agtcgccgcc cgaggccatc atgttcgggt ttatacccag tcgtgggaag gcgaatgccc
    12901 tgatgtattt gaactgatca aagtgccggt taaatcgcat accaatcacg ggcgcaatgc
    12961 ggaatatttt gcctgggtgc aaaaacattt acgtgaacac cccgtcgata gagttgttgg
    13021 atttaataaa atgccggggc tggacgttta ttatgccgct gatgtttgtt atgccgagaa
    13081 agtagcgcag gagaaaggct ttttctatcg cctgacgtca cgttatcgcc attatgctgc
    13141 ctttgagcgg gcaaccttcg aacagggcaa gccgacacag ctgctgatgc tgacagataa
    13201 gcaaatcgcc gatttccaga aacattatca gactgaagcg gaacgttttc atattctgcc
    13261 accggggatt tatcctgatc gtaaatatag ccagcagcca gccaatagcc gtgaaatctt
    13321 ccgtaagaag aatggaataa ccgaacaaca atatttattg ttgcaggttg gttcagactt
    13381 cacgcgtaaa ggtgtcgatc gttccattga agcacttgct tcgttaccgg attcgctgcg
    13441 ccacaacaca ttgctatatg ttgttgggca ggataaaccg cgaaaatttg aggcgctggc
    13501 agaaaaacgc ggcgtgcgca gtaatgttca cttcttctcg gggcgcaacg atgtctcgga
    13561 attaatggcg gcggcggatt tattacttca tcctgcctac caggaagccg cgggaattgt
    13621 actgctggaa gcgataactg caggattacc ggtactaaca acagccgttt gtggctatgc
    13681 gcattatatt gttgacgcca attgcggcga ggctattgct gagccattcc gccaggaaac
    13741 attgaatgag attttacgca aagcgttaac gcaatcttca ttgcgccagg cctgggcgga
    13801 aaatgcgcga cattatgctg atacacaaga tttatacagt ctgccagaga aagcggcgga
    13861 catcataacg ggtggtctgg atggttgaac ttaaagagcc gtttgccacg ttatggcgcg
    13921 gtaaagatcc ttttgaggaa gttaaaacct tgcagggtga ggtatttcgt gaactggaaa
    13981 ctcgccgcac tctgcgcttt gaaatggcgg gcaaaagcta ttttctcaaa tggcatcgcg
    14041 gcacgaccct gaaagagata atcaaaaatt tactctcatt gcggatgcca gtattaggcg
    14101 cagaccgcga atggaatgcg attcatcgac tgcgggatgt cggcgttgat actatgtatg
    14161 gggtggcatt cggcgaaaaa ggcattaatc cgctgaccag aacctcgttt attattaccg
    14221 aagatctgac accaaccatc agtctggaag attactgtgc tgactgggcg accaacccac
    14281 cagatgttcg cgtgaagcgt atgcttatta agcgtgtcgc gacgatggtg cgcgatatgc
    14341 atgctgcggg cattaaccac cgtgactgtt atatctgtca tttcctgctg cacttgcctt
    14401 tttccggtaa ggaagaggag ttaaaaattt cagtaattga cctgcaccgg gcgcagatac
    14461 gtgcaaaagt accgcgtcgt tggcgggata aagatttgat tggtttgtat ttttcatcaa
    14521 tgaatattgg gctgacgcag agagatatct ggcgatttat gaaggtttat tttgggatgc
    14581 ctttacgtaa gatattatct tttgaacgga atttattgaa catggcaagt gttaaggccg
    14641 agcatattaa agagcgaaca caacgcaaag gattataaaa atattaatca gtatgtactg
    14701 gaactaactt cattatgact atttatttta taaactgggt tgcagattat gaattaaaaa
    14761 tgatccagta cttgaaaaaa aagcacaaga taaaaaatat aacaacacca aaaaaataca
    14821 actgggttaa taagaaaatt tcgaaattag ggatggataa tgcatggttg ggacgattat
    14881 ttatcaaaca tcacctaaat gctgtaa
//
LOCUS       DAJOTB010000119        14156 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJOTB010000119 DAJOTB010000000
VERSION     DAJOTB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.119"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(268..1917)
                     /gene="pgi"
                     /locus_tag="OHN28_003569"
     CDS             complement(268..1917)
                     /gene="pgi"
                     /locus_tag="OHN28_003569"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCQ2298894.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2442..3791
                     /gene="lysC"
                     /locus_tag="OHN28_003570"
     CDS             2442..3791
                     /gene="lysC"
                     /locus_tag="OHN28_003570"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCQ2298895.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4044..4316
                     /locus_tag="OHN28_003571"
     CDS             4044..4316
                     /locus_tag="OHN28_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCQ2298896.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4317..5189)
                     /gene="rluF"
                     /locus_tag="OHN28_003572"
     CDS             complement(4317..5189)
                     /gene="rluF"
                     /locus_tag="OHN28_003572"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCQ2298897.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5537..6484
                     /locus_tag="OHN28_003573"
     CDS             5537..6484
                     /locus_tag="OHN28_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCQ2298898.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6554..7360
                     /locus_tag="OHN28_003574"
     CDS             6554..7360
                     /locus_tag="OHN28_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2298899.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7370..7777
                     /locus_tag="OHN28_003575"
     CDS             7370..7777
                     /locus_tag="OHN28_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCQ2298900.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7777..8271
                     /locus_tag="OHN28_003576"
     CDS             7777..8271
                     /locus_tag="OHN28_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCQ2298901.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8337..9134
                     /locus_tag="OHN28_003577"
     CDS             8337..9134
                     /locus_tag="OHN28_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCQ2298902.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9145..9969
                     /locus_tag="OHN28_003578"
     CDS             9145..9969
                     /locus_tag="OHN28_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCQ2298903.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNVVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10021..11250
                     /gene="sorE"
                     /locus_tag="OHN28_003579"
     CDS             10021..11250
                     /gene="sorE"
                     /locus_tag="OHN28_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCQ2298904.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11632..12321
                     /gene="pepE"
                     /locus_tag="OHN28_003580"
     CDS             11632..12321
                     /gene="pepE"
                     /locus_tag="OHN28_003580"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCQ2298905.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12411..14042)
                     /locus_tag="OHN28_003581"
     CDS             complement(12411..14042)
                     /locus_tag="OHN28_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCQ2298906.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3414 a         3575 c         3720 g         3447 t
ORIGIN      
        1 cacgattaaa aatttatttt aggattaacc tcacggtatg attttcgtta aatgacagac
       61 aagaggattt atctgataaa acaatgccga cttatagtcg gcattgtttt taaggctatt
      121 taatcaatcg tactatgtat taattgccga atacgatgta catatcggca tcgacctgta
      181 ggcctgataa gacgcgaccg cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc
      241 cttatccggc ctacatatcg acaatgatta accgcgccac gctttatagc ggttaatcag
      301 accattggtc gagctatcgt ggctgctgat ttctttgtca tctttcagct ctggcagaat
      361 acggttcgcc agttgtttac ccagctccac gccccattgg tcgaaggtga agatgttcag
      421 gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgccc ccaggctgaa
      481 cggagtgatt tcacgcagca ggatggagtt ggtcgggcgg ttaccttcga acactttgaa
      541 cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt cctgctcaac
      601 cacttcgcgg gatttaccaa acgccagcgc ttcggtctga gcaaagaagt tagacagcag
      661 tttctggtga tgatcagaga gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg
      721 taccattttg gttccctggt ggatcagctg atagaacgcg tgctgaccgt tcgtgccagg
      781 ttcaccccag ataatcgggc cagtctggta atccacaacc ttaccgttac ggtcaacata
      841 cttaccgttg gattccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg
      901 gtcatacggc agaatcgctt cagtttccgc gccaaagaaa ttgttgtacc agatgccaat
      961 cagcgccagc agtacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat
     1021 cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga gaacaatcga
     1081 caggccaatc gctgaccaca aagaataacg accgccgacc cagtcccaga attcgaacat
     1141 gttggcagta tcaataccaa actcgccaac ggctttggcg ttggtggaaa gcgccgcaaa
     1201 gtgtttcgca acgtgcttct catcacctgc cgctttcagg aaccagtcac gcgcgctatg
     1261 ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt
     1321 ttccgggttt acttttttca gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa
     1381 gtgcatgttc aggtggtttt tgtatggacg cagtgcttca gtcaccatgt acgggccgag
     1441 gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gccttgccgg tatagccttt
     1501 ccactcaccg gaaataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac
     1561 ttccggcata acatctttgc catcaaccag aatcggggta ttgctacggt tacgcagcgc
     1621 tacgtgcagt acggcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt
     1681 aatcgcgccc gccagatcgc actctttcgc cagatcttgt aatttcgcca gcgtctcttc
     1741 agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg cggagaactt
     1801 agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc
     1861 gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag
     1921 caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa
     1981 aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag cgcagcgaag
     2041 catttattag ctgaactact gaccgccagg tgtggatgaa aaatccgcat gaccccaccg
     2101 ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca
     2161 gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag
     2221 ggggtgcatc gccgaggtga ttgaacggct ggctacgttc atcatcggct acaggggctg
     2281 aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac
     2341 ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct
     2401 tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct
     2461 ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg
     2521 tgctttctga tgccaacgta cgtttagttg tcctctcggc ttctgctggt atcactaatc
     2581 tactggtcgc tttagctgaa ggactggaac ctggcgagcg attcgaaaaa ctcgacgcaa
     2641 tccgcaatat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag
     2701 agattgaacg cctgctggag aacattaccg ttctggcaga agcggcggcg ctggcaacgt
     2761 ctccggccct gacagatgaa ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg
     2821 tcgagatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc
     2881 gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg
     2941 cgctgcagct gctcccacgc ctcaatgaag gcttagtgat cacccaggga tttatcggca
     3001 gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct
     3061 tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct
     3121 acaccaccga tccacgcgtg gtttctgcag caaaacgcat tgatgaaatc gcgtttgccg
     3181 aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg
     3241 cagtacgcag cgatatcccg gtctttgtcg gctccagcaa agacccacgc gcaggtggta
     3301 cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca
     3361 atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggt ttcctcgcgg
     3421 aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc acatcagaag
     3481 tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga
     3541 cgcaatccct gctgatggag ctttccgcac tgtgccgggt ggaagtggaa gagggtctgg
     3601 cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat
     3661 tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc
     3721 tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcagaagcta cattttaatt
     3781 tgtttgagta aacacagtat ggcccggaag acattatttc gggccgtatt gtttttcttg
     3841 tcattatgcc cataaataaa cgagcctgta ctctgttaac cagcgtcttt atcggagaat
     3901 aattgccttt aattttttat ccgcatctct aactaattat cgaaagagat aaatagttaa
     3961 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
     4021 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
     4081 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
     4141 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
     4201 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
     4261 atcctgaggc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctgattaa
     4321 cgccccttct ttttgcgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg
     4381 cctttttccg ccgttttttc catcttaacg actggacgtt tgatgcctac cgttttcggt
     4441 ttggccttcg ctttaggttt tgcctcggaa gaggaatttt caatgagctt aaagagatcg
     4501 attaactcat cgtcggttaa atcgcgccac tcacccagcg gaatgccgct taagctgacg
     4561 ttcataatgc gcgtacgttc cagctttttc acttcgtaac caaagtgctc gcacatgcga
     4621 cgaatctgac ggttcagccc ctgcaccagg gtaatgcgga agacaaacgg tgcttctttt
     4681 ttgactttgc actttttagt taccgtcccg aggatcggca ctcccgcccc cataccacga
     4741 ataaattcat cggtaatcgg tttatcaacc gtcaccagat actctttctc atgatcgtta
     4801 cccgcacgga ggatcttatt cactagatcg ccgtgattgg tgaggaaaat cagcccctgg
     4861 gaatctttat ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca
     4921 atgttatcgc gctcgccatc ttcggtggtg ctgacaatgc ctacgggctt gttcagggcg
     4981 ataagtacca aatcttcggc ttcccgaggc tcaatcaact gaccgtttac tttcacaacg
     5041 tcgccggatt tcacctgatc gccaatggtg gctcgcttgc cattaaggaa cacattgcct
     5101 tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta
     5161 tttaaacgga ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt
     5221 acctcttagc ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca
     5281 cgccttttat cacaaaagtg tctccggaag tacgatgcaa atttgcctgg caaaataagg
     5341 ataatcaaca aattacgaat cgctgcacac ctgtgctgat gactactacg cagagaaaca
     5401 aatgtgcaag agaaagcgct atttcccccc atgcaaagag tgaaaaatcg ctttgaaagg
     5461 ccatcatgat ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca
     5521 gaaccaggag gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca
     5581 actctattac gaacaggata tgacgcaggc gcaaattgcg cgcgaactgg gtatttaccg
     5641 caccaccatc agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat
     5701 caactatgac tacaacgaaa atctctggct ggagcagcaa gtgaagcaaa agtttggcct
     5761 gaaagacgtt gtggtggtgt caggaaatga tgaggatgaa gatactcaac tggcgatgat
     5821 ggggttacac ggcgcgcaac tgctggatcg cttgctggaa cctggtgata ttgttggttt
     5881 ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg
     5941 gcagttaatt tgcgtgccga ttattggcgg cccgtccggt aaactcgaaa gccgctatca
     6001 cgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga
     6061 ttttccggct ctgctggata acccattaat tcgtaatggg atcatgcagt ctcagcactt
     6121 taaaaccatc tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc
     6181 ggccattcgc gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct
     6241 gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt tttgatattc acggcgcaat
     6301 ggttgaaacg aatatgagcg aaaaaacact ctctatcgaa atgaataaat taaagcaggc
     6361 acggtattcc attggcattg ccatgagcga agaaaaatac agcggaattg ttggtgcact
     6421 gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa
     6481 ataaaaatct tcccgatgtt taccgtaata aaaattacgg cgggtcctta ttatctaaaa
     6541 tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg
     6601 tggcgcatcc ggcattggtc tggcgattgt ggatgaatta ttagcacaag gcgcgaatgt
     6661 acagatggtc gatattcacg gtggcgatgg tcagtatgaa agccataaag gttatcagtt
     6721 ctggccgacc gatatttcca gcgccaaaga ggtaaatcat acggtagcag aaattatcca
     6781 gcgttttggt cgcatcgacg gtctggttaa caacgccggg gtcaatttcc cgcgtctgct
     6841 ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat
     6901 ggtcaatatc aaccagaaag gcgtttttct gatgtcgcag gcggtggcgc gacagatggt
     6961 caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga
     7021 aggccaaagc tgttacgccg cgaccaaagc cgcgctcaat agcttcacgc gctcctggtc
     7081 gaaagagctg ggtaagcacg gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa
     7141 aacagggctg cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt
     7201 cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt
     7261 agcagaagtg gctgattttg tttgttatct gctgtctgaa cgcgccagct atatcaccgg
     7321 agtaaccact aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta tggtcaatgc
     7381 cattttttgt gcgcatggca aattagcttg cgccatgctg gaatcggttc agatggttta
     7441 cggcgatgcc agggtcgagg cagttgaatt tgtgcccggc gagaacgccg gagacatcgt
     7501 agcaaagctg gaaaagttag taagcattca caatcaagat gagtggctga ttgccgtgga
     7561 tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct
     7621 gcgggtaatc agcggccttt ctctgccgct agcgctcgaa ctggttgata accaggacag
     7681 catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgtcgt
     7741 ctggagacag gtggcaacag cggaggagga tttctgatga acattacgct cgcccgcatt
     7801 gatgaccgcc tgatccacgg ccaggtcact accgtctggt ctaaagtggc taacgcccag
     7861 cgcattatta tctgcaatga cgaagtttat aacgatgaag ttcgccggac attattgcgt
     7921 caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtg
     7981 tatcacaacc cgcaatatca ggacgaaacc gttttttatc ttttcactcg cccacaggat
     8041 gccttagcaa tggttcgcca gggggtaaaa ataggtaccc tgaatattgg cggcatggcc
     8101 tggcgtcctg gtaaaaaaca gttaaccaaa gcagtttcat tagatgacga tgatattaac
     8161 gcatttcatg agttaaataa tcttggcgtc atactggatt tacgcgtcgt agcgtcagat
     8221 ccctcaatca atattatcga caaaataaat gaacagttaa tcgcgaatta aataatagcg
     8281 cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg
     8341 aaataagtac tctacaaatc attgctatat ttcttttttc ctgtattgcc ggaatgggca
     8401 gcgtgctgga tgaatttcag actcatcgtc cgttaattgc ctgtacggtg attggtttaa
     8461 ttctcggtga tttaaaaacc ggaattatgc tcggtggtac gctggaattg atagctctcg
     8521 gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc gctcgccagc ataatctccg
     8581 ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg
     8641 tggctgcggc aggccaggtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc
     8701 acgcggcgga taaagcagcg gaagaagcac ggtttcgcac tctcgatatt ctgcatgtct
     8761 ccgcgcttgg cgtgcaggca ctgcgcgttg ctattccggc actgattgtc tcgctgttcg
     8821 tcagcgctga tatggtgagc aatatgctga gcgccattcc cgaatttgtg acccgtggac
     8881 tgcagattgc tggcggtttt atcgtggtgg tcggttacgc catggtgctt cgcatgatgg
     8941 gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc
     9001 tcagtctgct ggcgttcggt ggcgtcggcg tgatcatggc cctgctctac atccagttaa
     9061 atccacagtg gcgtaaagct gaaccacatc cccagaccac cactatcacc gcccttgacc
     9121 aacttgatga ttaacggagc catcatggaa cagagaaaaa ttacacgcag cgatctggtg
     9181 agcatgtttc tgcgctccaa cctgcaacag gcatccttta actttgaacg tattcacggg
     9241 ctgggttttt gctacgacat gatccccgcc atcaaacgac tttacccatt aaaagaggat
     9301 caggttgcgg cgctcaggcg acacctggtg ttcttcaata ccacgccagc cgtatgtggc
     9361 ccggtcatcg gcgtcactgc cgccatggaa gaggcgcgag ccaacggcgc ggaaattgat
     9421 gacggtacca ttaacggcat caaagtcggt ctgatgggac cattggcagg agttggcgat
     9481 ccactggtct ggggaacgct gcgcccgatt accgccgcgc tcggcgcatc tctggcactt
     9541 tcgggcaaca ttctcggccc cctgctgttc ttctttattt tcaacgtggt gcgcctggcg
     9601 atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg
     9661 ggcggtaatt tgctgcaaaa actcaccgaa ggcgcgtcga ttctcgggct gtttgtgatg
     9721 ggcgtgctgg tgaccaaatg gacgtcaatc aacgtaccgt tggtggtttc acaaacgcct
     9781 gccgccgatg gtgccaccgt caccataacc gtgcagaaca tcctcgacca actttgccct
     9841 ggtttgctgg cgctcggtct gacgctgctg atggttcgtc tgctcaacaa aaaaattaac
     9901 ccggtatggc tgattttcgc cctgtttggt ttagggatta tcggtaatgc gctgggcttc
     9961 ctgtcctgat tcttcgcccc ggcgcaaccg tcggggccat cgttcaacac gaggtggttt
    10021 atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga aacctttgac
    10081 cttcctgaaa tgcaggagga tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc
    10141 tcttcctgga aagaggccaa tctgggtgaa aatcataaaa aagtacccga cgatgtggcg
    10201 accaatccca tcatcatcgg ccacgaattt tgcggcgata ttctggccgt gggtaaaaag
    10261 tggcagcaca aattccagcc gggtcagcgt tatgtgattc aggccaacct gcaactcccc
    10321 gaccgcccgg attgccccgg ctactccttc ccgtgggtag gcggcgaagc gacgcatgtg
    10381 gttattccca acgaggtcat ggaacaagat tgcctgctgg catacgacgg cgaaacctat
    10441 tttgaaggct cgctggttga accgctttcc tgcgtgattg gcgcgttcaa cgccaactat
    10501 catcttcagg aaggtagtta taaccacacg atggggattc gcccgcaagg gcgcacgctg
    10561 atcctcggcg gcaccggacc aatgggactg ttggcgattg attatgcgct acatggaccc
    10621 gttaaccctt cgctgctcgt cattaccgat accgacaacg acaaattgag ttatgcgcgc
    10681 aagcactatc catcagaacc gcaaacactg attcattatc tcaatgccgc cgatgcagca
    10741 tttgatacgt taatggcact gagtggcggt cacggcttcg atgatatttt cgtctttgtg
    10801 cctaatgaag gactggtgac tctcgcctct tccttgctgg cgacagatgg ttgtctgaat
    10861 ttcttcgccg gaccgcagga taagcacttc agcgcgccaa ttaatttcta cgatgtgcat
    10921 tatgcattta cccactacgt gggcacgtca ggcggcaata ccgacgacat gcgcgcagcg
    10981 gtcaaattaa ttgaagagaa aaaagtgcag gccgcaaaag tggtaacaca tattcttggg
    11041 ctgaatgccg cgggcgaaac cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg
    11101 gtgtataccg ggaaatacct gccgctgacg tcactcacgc agattcagga tcaagcactg
    11161 gcggcgattc tggcgcgtca tcaggggatc tggtccggtg aggcggagca atacctgctc
    11221 gcccatgcag aggcaatttc ccatgattaa tcgcgctact taactctgca tgtcgctggc
    11281 tggtcgccgc ggcaacttcg cgacctgatg tgcggctgat tgctgaagcg gcacaatgtg
    11341 cacgagaagg ataaaaaaac atcagcgcct taaggggcgc tcaaattggt ggcaaagtgc
    11401 acattgttta tgccggatgc ggaatgaagc cttattcggc ctacataatc ttgaaaattc
    11461 aatatattgc agttatctgg taggccaggt aagcgtagcg catcaggcaa tcctgtgttt
    11521 gtcatcagtc tcagcgcctg tgcaatatgt gacatcgtga gccatgtcag taagtggtta
    11581 aattcgcagt ggtagcattc cttccgtgtt ttcttactaa ccggagaagt aatggaactg
    11641 cttttattga gtaactcgac gctgccgggt aaagcctggc tggaacacgc gctgccgctg
    11701 attgctgaac agttgcaggg tcgccgttct gcggtattta ttcctttcgc tggcgtaacg
    11761 cagacctggg atgattacac agcgaaaacg gctgcggttc tcgctccgct gggtgtttct
    11821 gtcaccggta ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg
    11881 attgtcggcg gcggtaatac tttccagttg ctgaaacagt gccgcgagcg cgggctgctg
    11941 gcacccatta ctgacgtggt gaaacgtggc gcgctgtata ttggctggag cgcaggcgct
    12001 aaccttgctt gcccaaccat tcgtaccacc aacgatatgc cgattatcga tccgcaaggt
    12061 ttcgatgcgc taaatctgtt cccgctacaa atcaacccgc acttcaccaa cgcgctgccg
    12121 gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca
    12181 gaactgacga ttattggtct gccggaaggt aactggatca cagtaagtaa aggtcacgct
    12241 acgctgggtg gcccgaacac cacttatgta tttaaggctg gtgaagaagc ggtgccgctg
    12301 gaagcgggtc accgttttta agtgaattgc cggatgcatg atccggcatc ttgttcatag
    12361 tgcctgatgc aacgctttac gcgtcttatc aggccttcag ggtcacggta ttaatactcg
    12421 tcccgtcctt catcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc
    12481 gagttcagac gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatactc
    12541 tgcacgtttt gctggtgcag gcgatcgacg tgggcgtggg aatagcggcg attaagaatg
    12601 cgaaaacgat gtttgctgcg gcgcaaacga cgggcgctgg taacatcgcc agagaagaaa
    12661 accgacattg ccagttttaa attactgagc aattgctcat agagcgcatc cagttccttc
    12721 aagccatcaa gcgaaaatgc tcgccttgct gccagcgatt tgtcggcaat ttcgctgccc
    12781 atgcgctcga cgatatcgga ggcctgttca aggttgagcg acatttcgat gatctccgcc
    12841 cagcggcgcg actcttcttc tgccagttcc tcttttggca tccgcgccag atacagctta
    12901 atggcggtat agagaacgtt gatgtcatca gccagcttac gcagctcttt ctcctgccgt
    12961 ggctcgccgt gcatcacttt attcagccct tccatcatct gttccatggc gtcgccaatg
    13021 cgcagggttt cgcgggcagc gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca
    13081 tccagatgtt taggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg
    13141 caaaaccgtg ccatcgggtc aacgaatggc aacatgacca ggcagcgtac aaggttgtag
    13201 aagacgtgga aatagatcac caattccgct tttggcaatg gcaacttccc cattgtctct
    13261 gccagcaaat gaacaaacgg caggataatc aggctaccca ccagcttaaa cagcaggcta
    13321 cccagcgcga cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcagg
    13381 ccggaaccga ggtttgcacc aatcaccaga caaagcgcca ctgggaaaga gataatgcct
    13441 gcggcggtca gagtcgcggt cagcagtacc gcggcaaggc tggagtagct gataatggcg
    13501 aacatcgcac caatcagcgc atccagcaaa atgtcgccag tcagcgaggc aaagatcacc
    13561 tgaacgccgt ttgcctgagt gatcggcgtt acggcctgca caatcaactc cagcgccagt
    13621 aaaatcagcc caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt
    13681 ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg acagccagga taaatcgaag
    13741 gtgagaatac gcgccatcag cgccgtcccg acatctgcac ccagcacaat caccagcgca
    13801 ggcgcgagag caaccagatc ctgagcgacg aacgaggtca ccagcatggt ggtggcatta
    13861 ctgctctgca ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg
    13921 acgctccggc taaggacagt acgcagacgc gcgccgaaga cgcgcattac gcctgttcga
    13981 acaatatggg tcccccagac cagcagggcg acggcagaaa gcaggtgaag caaagttagc
    14041 acggaatcag gttctccttt ttgttatgca ttttcctgtt aagatttttc taatcacgtt
    14101 gtttttatcc aggcatttct cttgtatgta ctttcagtat aagagattta tacaag
//
LOCUS       DAJOTB010000120        14089 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJOTB010000120 DAJOTB010000000
VERSION     DAJOTB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.120"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..655
                     /gene="gspC"
                     /locus_tag="OHN28_003582"
     CDS             <1..655
                     /gene="gspC"
                     /locus_tag="OHN28_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2298907.1"
                     /translation="AFQSEFSLAALWRNENHTGVKDANPVAVNQETPKLSIALNGIVL
                     TSNDETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPG
                     LPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALR
                     INNLSLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            639..2591
                     /gene="gspD"
                     /locus_tag="OHN28_003583"
     CDS             639..2591
                     /gene="gspD"
                     /locus_tag="OHN28_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2298908.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2601..4082
                     /gene="gspE"
                     /locus_tag="OHN28_003584"
     CDS             2601..4082
                     /gene="gspE"
                     /locus_tag="OHN28_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCQ2298909.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4079..5275
                     /gene="gspF"
                     /locus_tag="OHN28_003585"
     CDS             4079..5275
                     /gene="gspF"
                     /locus_tag="OHN28_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCQ2298910.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5285..5722
                     /gene="gspG"
                     /locus_tag="OHN28_003586"
     CDS             5285..5722
                     /gene="gspG"
                     /locus_tag="OHN28_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCQ2298911.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5730..6239
                     /gene="gspH"
                     /locus_tag="OHN28_003587"
     CDS             5730..6239
                     /gene="gspH"
                     /locus_tag="OHN28_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCQ2298912.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6236..6613
                     /gene="gspI"
                     /locus_tag="OHN28_003588"
     CDS             6236..6613
                     /gene="gspI"
                     /locus_tag="OHN28_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCQ2298913.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6606..7193
                     /gene="gspJ"
                     /locus_tag="OHN28_003589"
     CDS             6606..7193
                     /gene="gspJ"
                     /locus_tag="OHN28_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCQ2298914.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7186..8169
                     /gene="gspK"
                     /locus_tag="OHN28_003590"
     CDS             7186..8169
                     /gene="gspK"
                     /locus_tag="OHN28_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCQ2298915.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8184..9347
                     /gene="gspL"
                     /locus_tag="OHN28_003591"
     CDS             8184..9347
                     /gene="gspL"
                     /locus_tag="OHN28_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCQ2298916.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9344..9805
                     /gene="gspM"
                     /locus_tag="OHN28_003592"
     CDS             9344..9805
                     /gene="gspM"
                     /locus_tag="OHN28_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HCQ2298917.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9805..10482
                     /gene="gspO"
                     /locus_tag="OHN28_003593"
     CDS             9805..10482
                     /gene="gspO"
                     /locus_tag="OHN28_003593"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HCQ2298918.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10511..10987)
                     /gene="bfr"
                     /locus_tag="OHN28_003594"
     CDS             complement(10511..10987)
                     /gene="bfr"
                     /locus_tag="OHN28_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCQ2298919.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11059..11253)
                     /gene="bfd"
                     /locus_tag="OHN28_003595"
     CDS             complement(11059..11253)
                     /gene="bfd"
                     /locus_tag="OHN28_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCQ2298920.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11422..14079)
                     /gene="chiA"
                     /locus_tag="OHN28_003596"
     CDS             complement(11422..14079)
                     /gene="chiA"
                     /locus_tag="OHN28_003596"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HCQ2298921.1"
                     /translation="MCSSLPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPG
                     KAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSNTPASNTPANGGSATP
                     AQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRY
                     VRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWK
                     GQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNP
                     GSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGS
                     EIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEI
                     DNGGVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAW
                     SASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKTQSYSNE
                     ELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVG
                     TPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAY
                     GMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRV
                     LDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVL
                     SDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHAQLKGLYGNK
                     SDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGT
                     NLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3616 a         3632 c         3349 g         3492 t
ORIGIN      
        1 tgcatttcag tctgaattca gccttgccgc tttatggcgc aacgaaaatc atactggggt
       61 aaaagatgca aatcccgtgg cagttaatca ggaaacacct aagctctcta tagcgcttaa
      121 tggcattgta ctcacatcga atgatgagac ttcttttgtc ttaattaatg aggggaacga
      181 acaaaagcga tactccctca acgaagccct tgagtcagcg ccgggaacgt ttattagaaa
      241 aataaataaa accagcgttg tgtttgaaac tcacgggcat tacgaaaaag taactctcca
      301 tccagggcta cctgacatca tcaagcagcc tgattcggaa aatcaaaatg tactcgccga
      361 ttatattatc gccacaccta tacgtgatgg agaacagata tacggtcttc gattgaatcc
      421 tcgtaagggt ctcaatgcat ttaccaccag tttgctgcaa cctggggata tcgccctcag
      481 gatcaataat ctttcgctga cccaccctga tgagatttca caggcattaa gtttactgct
      541 aactcaacaa agtgctcagt ttacaattcg tcgcaacggc gtaccccgct tgataaatgt
      601 ttccgtcgcg gaacttacag gaatgaatgg acagcgcaat gaagggactc aataaaatca
      661 cctgctgctt gctggcagca ttactcatgc cttgtgcagg acacgctgag aacgaacaat
      721 acggcgctaa cttcaataac gccgatatcc gccagttcgt ggaaatagtg ggtcagcatc
      781 ttggcaaaac gatcctgatc gacccttcgg tacagggaac catttccgta cgcagtaatg
      841 atacgtttag ccaacaggag tactaccagt tctttttaag tattcttgat ctttacggtt
      901 attccgtgat cacgctggac aatggttttc tgaaagtggt gcgctcagct aatgtaaaaa
      961 catcgccagg gatgattgct gacagttctc gtccaggcgt aggtgatgag ttggtcaccc
     1021 gaatcgtacc gcttgagaac gttcctgctc gtgacctggc tcccctgctc cgccagatga
     1081 tggatgcggg tagcgtcggt aatgttgtgc attatgaacc ctccaacgtt cttattctga
     1141 ccggtcgtgc ctccaccatt aataaactga ttgaagtcat aaagcgcgtt gatgtcatcg
     1201 gcacagagaa gcagcaaatt attcatctgg aatatgcatc agcggaagat ctcgccgaga
     1261 ttcttaatca attaatcagc gaaagccacg gtaaaagcca gatgcctgcc ctcctttccg
     1321 cgaagattgt ggcggataag cgaaccaact ctcttatcat cagcggaccg gaaaaagcac
     1381 gccagcgcat aacttcatta ctgaaaagcc ttgatgtaga agagagcgag gaaggaaata
     1441 cccgggtcta ttacctgaaa tatgctaaag ccacgaatct ggtggaagtg ctaaccggtg
     1501 tttccgaaaa gctgaaagat gaaaaaggga attcgcgtaa gccttcttct acttctgcga
     1561 tggataatgt ggccattacc gccgatgaac agaccaactc gttggtcatt accgctgacc
     1621 agtccgtcca ggagaaactc gccacggtaa ttgcgcgtct ggatattcgc cgtgcacagg
     1681 tgctggttga ggcaatcatc gtcgaagttc aggatggaaa tggactcaac ctcggcgtgc
     1741 aatgggcgaa taaaaacgtt ggcgcacagc aatttaccaa tacgggatta ccggttttta
     1801 acgctgcgca aggtgtagct gattataaaa agaatggcgg gatcaccagc gcgaatcctg
     1861 cctgggatat gtttagcgcc tacaatggca tggctgcagg cttcttcaat ggcgactggg
     1921 gcgtattgtt gactgcgctg gccagtaaca ataaaaatga catcctcgcc accccgagca
     1981 tcgtaacgct ggataataaa ctcgcgtcct tcaacgttgg tcaggatgtg ccggtgctat
     2041 ccggctctca gaccacttct ggggataacg tctttaatac tgtcgaacgc aaaacggtgg
     2101 gcacaaaact caaagtcact ccgcaggtca atgaaggcga cgcggtactg ctcgaaatag
     2161 aacaggaggt ttccagcgtt gactcttcgt ctaattcgac gctcggcccg acatttaaca
     2221 cccgtactat tcaaaacgcc gtgctggtta aaaccggtga aacggtggtc ctgggcggat
     2281 tgctggatga tttttctaaa gagcaagtgt caaaggttcc tctgcttggc gatattcctt
     2341 tagtgggaca actcttccgc tatacctcga ccgagcgcgc taaacgcaac ctgatggtat
     2401 tcatccgccc gacgattatc cgtgacgatg atgtttatcg ctcactgtca aaagagaaat
     2461 acacccgcta ccgtcaggag caacagctgc gaatcgacgg gaaatcaaaa gcgctggttg
     2521 gctcggaaga tttgccggtg ctgaatgaga acacgttcaa cagtcacgcc cctgcgccat
     2581 cgtcacggtg aggcattctt atgagaattc actcaccgta ccccgccagt tgggcgctgg
     2641 cacaacgaat tggttatctc tattcagagg gcgagattat ttatctcgcc gatacgccat
     2701 tcgaacggtt actcgatatt caacgtcaag ttggtcagtg ccagaccatg accagcttgt
     2761 cacaggctga ttttgaagcc cgactggaag cggtgttcca tcagaatacc ggtgagtcgc
     2821 aacagattgc gcaggatatc gatcaatccg tcgatcttct ctcgctttcg gaagagatgc
     2881 ccgcaaatga agatctcctg aatgaagatt cagcggcacc ggttatccgc ttgatcaatg
     2941 ctattttgag tgaggccatc aaagaaaccg cctccgatat ccacattgaa acctatgaaa
     3001 aaacaatgtc gatccgtttt cgcatcgacg gcgttttgcg gacaatttta cagcccaaca
     3061 aaaaactggc ggcactgctt atctcccgaa ttaaggtcat ggctcgtctt gatatcgccg
     3121 aaaaacgtat tccacaggat ggaaggatca gtttgcgtat cgggcgacgt aacatagatg
     3181 tccgcgtatc cacactgcca tccatctatg gtgaacgcgc tgtgctccgt ctgctggata
     3241 aaaacagcct ccagctttca ttgaacaacc tggggatgac ggcagcggat aaacaggatt
     3301 tagaaaatct cattcagctt ccgcacggta ttatcctggt gacagggccg acaggctccg
     3361 gtaaaagcac cacgctctac gccatccttt cggcgcttaa tatccctggc cgtaatattc
     3421 tgacggtaga agatcccgtg gaatatgagc tggaaggcat tggacaaacg caggtgaata
     3481 cccgtgtgga tatgtctttc gctcgcggcc tgcgcgccat acttcgccag gacccggatg
     3541 tcgtcatggt gggggaaatt cgtgatacag aaaccgcgca aattgcggtt caggcctcgc
     3601 tcacaggcca tctggtactc tcaacactcc acactaacag tgcatcaggc gcagtcaccc
     3661 ggctacgcga catgggcgtc gaatcattcc tgctttcatc ttcactggca gggattatcg
     3721 cgcaacgtct ggttcgtcgt ctgtgtccgc aatgccgaca attcacgccc gtatcgcccc
     3781 agcaagcgca gatgtttaaa tatcatcagc tcgcggtgac aacaattggc actcccgtag
     3841 gttgccctca ttgccatcaa tccggttatc aggggcgcat ggcgatccac gaaatgatgg
     3901 tggtgacgcc ggaattgcgg gccgctattc atgaaaatgt ggatgaacaa gcactggagc
     3961 gactggtccg gcaacaacac aatgccttaa tcaaaaatgg cctgcaaaca gtgatacgcg
     4021 gtgacacctc ctgggatgag gttatgcgcg tcgccagtgc tacgctggag aacaaagcat
     4081 gaattatcgc tatcgcgcca tgacccagga tggtcaaaaa ttgcagggga tcattgatgc
     4141 taacgatgaa cgtcaggcac gactgcggct gcgtgaagaa gggcttttcc tgttggatat
     4201 tcgcccccaa aaaagttcgg gagtaaaaac acgtcgcccg aggatcagcc atagtgaact
     4261 gacgcttttc acccggcagt tggcaacctt aagcgcagcg gcattacccc tggaagagag
     4321 ccttgccgta attggtcaac aaagcagtaa taaacgactg gctgacgtgt taaatcaggt
     4381 acgcagcgcc atccttgaag ggcatcccct ttcagatgca ttacagcatt tccccacgct
     4441 tttcgattcg ctctaccgta ccctggtaaa agcgggcgaa aagagcggtc tgctggcccc
     4501 ggtgttggaa aagctggctg attacaatga aaaccggcag aaaatccgca gcaagctcat
     4561 tcagtcactg atctacccct gcatgctcac tacggtggcg attgtggtcg tgattattct
     4621 cctcactgct gtcgtgccca aaattaccga acagttcgtg catatgaagc agcaactgcc
     4681 actgagtaca cgcattcttt taggtctgag cgacacgttg caacgtaccg gcccgacatt
     4741 attagcgaca gtatttattg tcgctgtggg tttctggctc tggttaaaac gcggcaataa
     4801 ccgccaccgt tttcatgcca tgttactgcg cgttgcgctc atcggcccgc tgatttgcgc
     4861 cattaacagc gcacgctatc tccgcacttt aagtattttg caatccagcg gcgtccctct
     4921 gctggatggg atgaatttgt ccaccgaaag cctcaacaac ctcgaaattc gccagcgcct
     4981 ggcaaatgcg gcagaaaacg ttcgccaggg taacagcatt catctttcac tggaacaaac
     5041 cgcaattttc ccgccgatga tgctctacat ggtggcctct ggcgaaaaaa gcgggcaact
     5101 cggcacgtta atggtcagag ccgcagataa ccaggagaca ctccaacaaa atcggatcgc
     5161 cttaacgctc tccatcttcg agccagcact cattattacg atggcactga tcgtcctgtt
     5221 tattgtcgtg tcggtactcc aacctcttct tcaacttaac tcaatgatta attaaggaaa
     5281 tcatatgcgc gcaacggata agcaacgcgg ttttacatta ctggaaatta tggtggtaat
     5341 tgtcatcatt ggcgtactcg ccagcctggt ggttcccaac ctgatgggca ataaagaaaa
     5401 ggcggataaa caaaaagccg tcagcgatat tgtggcgctg gaaaacgccc tggatatgta
     5461 caagctcgac aatcatcgct atccaaccac aaatcagggg cttgaatcct tagtagaagc
     5521 gccgacactg ccaccgctgg ccgcaaacta taacaaggaa ggttatatca agcgtctgcc
     5581 tgccgatccc tggggcaatg attatgtcct cgttaatcct ggtgaacatg gtgcatacga
     5641 tctgctttca gcaggacccg atggtgaaat gggaaccgag gacgacatca ccaactgggg
     5701 tttgagcaag aagaaaaagt aagtcagcga tgaatcagca acgcgggttt accctgctgg
     5761 agatgatgct ggtgctggcg ctggtcgcta tcacggcaag tgtggtgctc ttcacatatg
     5821 gtcgggagga tgccgccagc actcgtgcgc gcgaaacagc ggcgcggttt accgctgcac
     5881 ttgaattagc catcgaccgc gccacgctca gtggtcagcc tgtcggtatc catttctctg
     5941 attcagcatg gcgcattatg gtaccgggca aaacaccatc agcctggcgc tgggttccat
     6001 tacaagaaga tgccgcagac gagagcaaaa atgactgggg tgaagaactc tcaattcaac
     6061 ttcagccatt taaacctgat gacagcaacc aaccgcaggt ggtgattctg gccgacggac
     6121 aaatcacgcc cttctctttg ttgatggcta ataccgggac cggtgagccg ctcctgacgt
     6181 tagtcagttc aggctcatgg cccctggatc aaacgctcgc cagagatacc cgcccatgaa
     6241 caaacaatca gggatgacac tgcttgaagt cttactggcg atgagtattt tcaccgctgt
     6301 tgcgctgact ttaatgagca gtatgcaagg gcaacgaact gccatcgagc ggatgcgtaa
     6361 cgagacgctg gcattgtgga tagcggataa ccagcttcag tcacaagatt cattcgacga
     6421 ggaaaacacg tccagttctg gcaaagagtt aataaatggc gaagagtgga actggcgcag
     6481 cgatatccac tcaagcaaag acggcacgct tctggaacgc accataacgg tgacattacc
     6541 cagcggccag acaacctcgc ttacgcgcta tcaaagcatc aataataaat ccgggcaggc
     6601 acaagatgat taatcgccag cagggtttca cattgctgga agtcatggcg gcgctggcga
     6661 ttttctcaat gcttagcgtg ctggcgttta tgattttttc tcaagcttcc gagttgcatc
     6721 aacgttcaca gaaagaaatc cagcagttca atcagctaca gcgcaccatc accattcttg
     6781 ataacgatct cctgcaactg gtcgcccggc ggaatcggag tacggacaaa atcatggtgt
     6841 tgggtgaaga agcgattttt accacgcaaa gtcgcgatcc gctggcccct ctcagtgaag
     6901 cacaaacctt actcactgtt cactggtatc tgcgaaatca tacgctctac cgtgctgttc
     6961 gtacttctgt ggatggcagg aaggatcaac ccgcccaggc gatgctggaa catgtcgaga
     7021 gctttcttct ggaaagtaac agcggagaaa gccaggagct tcctctctca gtcacgttac
     7081 atctgaagac tcagcaatat ggggcgttac agcgccgttt tgcattacct gagcagttag
     7141 cgagagaaga gtcgccagct cagacgcagg ccgggaataa taaccatgaa taatgaacaa
     7201 cgcggcgtag cactgctcat tgtactcatg ttactggcgc tcatggctgc tctggctgca
     7261 gacatgacga tcagttttca cggccagctt catcgaaccc gccaggtaaa ccatcatttg
     7321 caacggcaat atgatattga gctggcggag aaacttgcgc ttgccagcct cacccaggac
     7381 gttaaagata acgacagaca aaccacgttg cagcagtact gggcacaacc gcaacagttg
     7441 cagctggaaa atggcaatac agtgaagtgg cagttacgcg atgcccagca ctgttttaat
     7501 ctcaatgcct tagcaaaaat ctctgacgcc ccgctggctt cccctgattt ccccgcccag
     7561 gtattcagtg ccctgctgat taacgccggt atcgatcgcg gaaacaccga tgagatcgtg
     7621 caatcaatcg ctgactatat tgatgccgat gattcgccac gctttcacgg cgcagaagat
     7681 aacttttacc aaagccaaac gccgcctcgc catagcgcca atcaaatgct ttttctgacc
     7741 ggtgaattgc gtcagataaa aggcataacc gaaaacatct atcagcggct tatcccgtat
     7801 gtctgcgttc tcccgacctc ggaacttagc atcaatctta atatgctaac ggagaacgat
     7861 attccgctct tcagagcatt attcctcaat aacatcacgg atgctgacgc tcgggtctta
     7921 ttacaaaaaa ggcccaggga aggctggcta accacagatg catttcttta ctgggcgcag
     7981 caggatttct ccggcgtgaa gcccctggtt gctcaggtga aagggcatct ctttccctat
     8041 agccgctatt ttacgctgag tacggaaagt atcagcgacg aacaatccca gggatggcaa
     8101 agccatattt ttttcaaccg taaacagcag agcgcccaaa tctatcggcg tacgctgcaa
     8161 ctctactaat cataaggatc gtgatgcctg aatcgttgat ggtaatacgt tctttctcga
     8221 cgctgcgtaa gcattgggaa tggatgacgt tctccgcaga tagcgtatct tcggtacata
     8281 cgctcacaga cgatcttccg ctggggtcgc tggccgatca gcccggtgcc ggaaatgttc
     8341 accttttgat cccacctgag ggtcttttat atcgttccct cacgctgccc aacgccaaat
     8401 acaaactcac cgcgcaaaca ttacagtggt tggcggagga gacattaccc gacgccagcc
     8461 aaaattggca ctggacggtt gtcgataaac aaaacgaaag tgtcgaggtg ataggcattc
     8521 agtcggagaa actcagtcgt tatcttgagc gtttacatac tgccggactc aacgtgacgc
     8581 gggttttgcc agatggctgc tatcttccct gggaagtaga tagctggact ctggttaacc
     8641 agcaaacaag ctggcttatt cgcagtgccg cacatgcctt caacgaactg gatgaacact
     8701 ggttacagca tcttgctaac cagtttccgc ctgagaatat gcgttgttat ggtgtagcgc
     8761 ctcacggcgt tgcagcggcc aatccactta tccagcatcc tgaaatacca tcactgagtt
     8821 tgtattctgc cgatattgca ttccagcgct atgacatgct gcatggtgtt tttcgtaaac
     8881 agaaaactgt aggcaaatcg ggaaaatggc tcacccgcct ggcagtaagc tgtctcgttc
     8941 tcgccattct ttcttttgtt ggcagccggg gcatcgcgct ctggcaaacg cttaagattg
     9001 aagatcaact tcagcaacaa cagcaagaaa cctggcaacg ttatttcccg cagatcaaac
     9061 acacccacaa ctttcgtttt tactttaagc agcaactcgc tcagcaatat cctgaagctg
     9121 taccgttgct ctatcattta caaacacttc tgcttgaaca ctctgaactg cagttaatgg
     9181 aggcgaacta tagccagagg caaaagtcgc tgaccttaaa aatgagtgct aagagtgaag
     9241 ccaatatcga tcgtttctgt gagttaacac agtcctggct tccgatggaa aaaacagaaa
     9301 aagatccggt cagtggtgta tggacagtaa ggaactcagg gaaatgatta aatcatggtg
     9361 ggcagaaaaa tccacatcgg aaaaacagat cattgcggca ctggcggttc tctcgctcgg
     9421 cgttttttgt tggcttgcgg tgataaaacc gatagatacc tacattgaag agcatcagtc
     9481 ccacgcgcaa aaaattaaga aggacatcaa atggatgcag gatcaagcga gcacccatgg
     9541 tttactcggt catccagcgc ttacgcagcc catcaaaaat atccttctcg aagaagcaaa
     9601 acgtgagaat ttgactatca cgctggaaaa cggtccggac aatactctga caattaatcc
     9661 tgtcacagct cccctcgaaa acgtctcccg ctggctcact acggcacaga taacatacgg
     9721 tattgttatt gaagaccttc agtttactct tgccggaaac gaagagatca ccctcaagca
     9781 tttgtccttc agggagcaac aataatgaca atgttactgc cgctcttcat tctggttggt
     9841 tttattgcag gttattttgt taatgccatg gcctatcacc tctcgccttt ggaggataaa
     9901 acggcgttaa cgtttcgcca ggtattggtt catttctggc aaaaaaaata tgcctggcat
     9961 gatacagtgc ccctgatact ttgcgttgct gctgctatcg cctgcgccct ggcacctttc
    10021 acgcccatcg tgactggcgc actctttctc tatttctgtt tcgcactcac gctcagtgtt
    10081 attgattttc gcactcagct cctgcccgac aaactcacct taccgctgct ctggcttggc
    10141 ttggtattta atgcgcagtc tggattaatt gatttacatg atgcggttta cggcgcggtc
    10201 gctggctatg gggtgctgtg gtgtgtttac tggggcgtct ggttagtttg tcacaaagag
    10261 ggattgggct acggtgattt caagctactg gccgctgcag gcgcgtggtg tggctggcaa
    10321 acgttgccaa tgatactgct gattgcctcg ctgggtggca ttggttacgc catcgtttca
    10381 caacttctgc aacgccggac tataaccact atcgcattcg gcccgtggct tgcgctcggc
    10441 agcatgataa acctgggata tctggcctgg atctcttatt aaccgggagg gttctccctc
    10501 ccgacacggc tcaaccttct tcgcgaatct gtgcttgcag ataattttgc agtcccatct
    10561 tctgaatcag atcaagttcc gtttccagcc agtcgatatg gccttcttca tcgcgcaaaa
    10621 tttctatcat catatcgcgg ctgacgtaat catgaacgct atcggcataa ccaattgcct
    10681 cacgcaaatt cttcgcgcca tccagctcaa gtgccagatc agaacgcagc atttcctcaa
    10741 catcttcacc aatgttcagt ttgcccaggt cctgtaagtt tggaagacct tccagaaaaa
    10801 gaatgcgctc tatataacga tcggcgtgtt tcatctcatc aatggattca tgatactcca
    10861 catcattgag acgtttgaga ccccagtttt taaacatccg ggcatgaaga aagtactgat
    10921 tgattgcgac aagctcattt cccaacagtt tgttgagata atttataact ttagtatcac
    10981 ctttcatttt ttgactcctt cgcttccact ctttgaagcg tagaacggct tacgagtaag
    11041 tcaaaaaaca cactccgttt atgcggactc cttaaactcc ggcagttgca ttaattcatc
    11101 ctccatcact tcacgcgcgg cccggacaca tttaccgcac tgattgccga ccggaatgaa
    11161 tttttttaat tgctggaacg agtgggggga aaactggcgc acagcctgac gaattttttt
    11221 atcgctgata ccattacaaa gacaaacgta catagagact cccgtatact ttcttcaaaa
    11281 gtaaatgaaa atagttctta tttcaattgc aggaagactc ttttttaagc aaagaataga
    11341 aatatgaata aaaataatgg gctaccaaaa gtagcccatt gacaaaaaat gcggcgatac
    11401 cggaaggtat cgccaacaca tttattgctt agtaaacggc gcgaagattt cgctaaatgc
    11461 gtacttcgga gcctgttctt tagtcagacc gtggaattcc ggagacaggt tagtgccgcc
    11521 cggcaggtcg cgcgcgattg accagatacc aaccataccg agattacgct tctgcgcatc
    11581 ctgcatgacc agacgagcat cagagagata gaacacctca ccctgaacgt cgttcacgcc
    11641 aaccatcggc gtggtaccca tcatggcgtc aatttctgca tcactcttat tgccatagag
    11701 gcctttcagc tgcgcatgca ggttggcaat cgcagacgtt gcacacttac cgtggatgtt
    11761 ttggccttcg gtgtttgcag actgacaaat agcgttaccg tagtccattg tcatcacgtt
    11821 cacacccgcc agctcaacac ctttcgcttt ggcatcgctc aggacattca tcccttccgg
    11881 cgtcaggcct gtcggcagaa ttggcaaggt ataccagata gcaatatctt tgccttctga
    11941 cttccattta tcctgcactt tcttcacagc aaggttacga cgttcaatag atgcctgatc
    12001 cgcaacccag gtgccttcga tatcgaagtc caggactctg aggttcaggt tatcaacgat
    12061 gtcataataa tgctgcatta gatcgtcaac gttcttacag gaagctgcca gcggggcatt
    12121 attagcacca ccgatagaca gcatcacatc accgccagcc tcacgcagcg ctttgatttt
    12181 gctgtactga gcgtaattct gcataccgta agcggtcccc catgtcggca gacaggtgtt
    12241 cgcatcttta ctcaccacaa acgccagcgt gaagtggttg acgttatgat tcttagccag
    12301 cgcagccaga tccgggatcg tattcagcgt aaagtcgaca tacggtgcat aaacgtgttt
    12361 cggccacgct ttcttcggcg tacctacgcg ctctttggtt ccggtccagt caacaaagac
    12421 acgccacggg ttgctgtcag aaggagaaac tttctgcact ttactctgag aaatgtagtt
    12481 ctcaccgtta aagcgaatca gcgtatcact ggcataaagc gtttctggat taaactgcgg
    12541 cgcattattc agctcttcgt tgctatagct ctgagtctta cctaacggct tccacgggcg
    12601 actattgcta ccggtggcgt tttggttcgc aaccagagca gggttgtcac tttgcgtcca
    12661 gaacagcgct tcataagcct gcccatcaac aacaacgcga tcacctttca cgtacacggt
    12721 ggaagcggac catgctttcg ccggttcgta tttcttccac gggttatcgc cgccggggac
    12781 aaagccccat gcgtcttgat cgacagaggt tttataatgt gcatcgttat aatctacgat
    12841 gctgttcgcg ctatacgctt tgctggcctg gaaaccatcc acaataatga cgccgccatt
    12901 atcaatttca caggtagtcg ggttaccgta ctgactcatt tccgcagcgg tagctgtacg
    12961 cacgagacgc catgggttat tggagttttc tacactggtg ccacgcgggc aatcttcaga
    13021 accaacccac caggcattct gataaacatt accattgaag ataacatggt aaatttcgct
    13081 gccttcctgg ccactccacg cctgcagtga gtaatcgtta ctgctatccg ctggtgttga
    13141 tggctcgttg ttatccggag ttggagtaac tggagtttcc ggtgtttccg gagttggatc
    13201 aaccggagtc acagcaccgc cattattatc cggcttaacg gaacaggagc ctgggttacc
    13261 atactgagtg atttctgttg ccgttgccgc gcgaacataa cgccatgggt tgctggcatc
    13321 gttttctttc gcatcgcctg ggcaatttgc cgcgcctacc caccaggcgt tcttataaat
    13381 gctgccgttg tagataacgt accatgtaga agaaccttcc tgacctttcc aggctacaac
    13441 agcatagttg tcgtttgact tatcagctgg ggctggctga acatcagggg ccggtttaac
    13501 gtccggtgcc ggtttcacat ccggtgcagg ctgtggtgct gaagtacaag actgtggatt
    13561 actggtttct gagatttccg ttgccgtagc ggcacgaaca taacgccatg ggttgctggc
    13621 atcattgcct ttcgcatcac ccgggcagtt agaagaggct acccaccagg catttttata
    13681 taccgcacca ttaaagacga cataccaggt ttgaccgccc tgctgtttat tccaggcaac
    13741 tacagaagaa ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagcggg
    13801 cgtattagat gctggcgtat ttgaagttga agaagatgag cttccgctct tttcgcagga
    13861 aagtgtattg ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg
    13921 gttagttgca tcatttgctt tcgcttttcc cgggcaattt gtagaagaaa cccaccaggc
    13981 attttcataa attttgccat cgaaaataac ctgatattta ttaccacctt gttgattatt
    14041 ccatgcttcc attgccaatg ctggcagaga ggaacacacc agccccata
//
LOCUS       DAJOTB010000121        14028 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJOTB010000121 DAJOTB010000000
VERSION     DAJOTB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.121"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            31..426
                     /locus_tag="OHN28_003597"
     CDS             31..426
                     /locus_tag="OHN28_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298922.1"
                     /translation="MIAILNKAKGRVGVRGQAVIIILLGLIVAKLYPTLSMVFFAIIG
                     AAIFTYIIKHTALWFYKIDIIQKFVRPVSMLLLALIPIGVAYVIDVFYPSDTERWLIV
                     LYCYIGVALFCGFHWCFEKINDSISKNKD"
     gene            complement(446..2563)
                     /locus_tag="OHN28_003598"
     CDS             complement(446..2563)
                     /locus_tag="OHN28_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298923.1"
                     /translation="MAANVILPEGFVLDEQPDNSQFPDSSQLPDGFVLDAQPEQQQSP
                     LVSPEENSRQENVVNNANGFDRFMYGVLSGLMDVGKGVGLFQDMTPEEQAAIQSLQQK
                     LAAKPSTAQDVGEFVGQAAPFVSGGGIISQVPKGAARLAAAAGLGAGEGAIVANGTNS
                     DVASGAAIGAVAGPVAELAGPAINKIAGKLTKKNVSSIPDIEGITPAEEKIRKLSAQG
                     NPNLASSLEGLDLKPQKEIVEAANRLHVDSVLPSHLSGNEQYQAVEQALKSRTGSALK
                     AQEDKAISELASSAGKIIDDVAGAPDALAMSEKYIYQINSRMEALKNRSDQLYKRVDS
                     ALSPRTKVEANNTSTMLEKIADDLGGWDNLDPIEKRVFTAINPGPDSILTYANLNRQR
                     RLVGDALNKKQGPYKDADKEALSRLYGALAEDQKSVLSNTGYLRDFEVAQRLVAMRKN
                     LERQMVNLRGKTLNGDIAYKVTNSLQSMAKGDARGFREIMQNTPSRTLRRELVGTALR
                     DMLSSGKRGADFNPSGFADWWQNLQHSGQLKTLAEHLPKETMSGLYDVYKVARAIKNA
                     KAHEISTGKLNEFVNRFNRVTAPYELAAKHIQKIGTMVGAHFGSLGAIAGADIGARLA
                     SKARLAGGAGAADAADKLIASPAFQSAVKNLHGKAPGHIVDSRIRRSPQWKSFFNSLP
                     EQEKRTIARLGIITWLSNDPDND"
     gene            complement(2548..3846)
                     /locus_tag="OHN28_003599"
     CDS             complement(2548..3846)
                     /locus_tag="OHN28_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCQ2298924.1"
                     /translation="MATWQLGGLPSMAPQNDNAPRSSVPQPVQYQQQPNVGLMALQGL
                     RGVAEINQQARQQQRKAEFQKAYAGAFESGDRNKMRSLISEYPEEFESVQKGMGFIDD
                     DQRNSIGHLATSAQIASSLGTGAFGKFIADNEDEMRRLGISPESVAEMHVNDPQGFQR
                     LAGSMALFSLGHEKYFDIKDRMEGRRLEQGRLDESMRQADMENARGWANIQNAQLDRA
                     QRAQIHSDEMGLKLMELGQKGKPSADLIKGLNSDITNFGKNYNSVRAAANSLQALSKV
                     NTGAAQLGIIFNYMKSLDPQSVVREGEQVQVMRSDGIWGQIKGYIDQLNAGNGLSQEA
                     KDNIVNAAKINANAMGQQFNQQVDEYLDTYGETIPQGLRKSLERRKAKLFDDVSGQPT
                     SQAGTGQAKAATGGISEGATATNSKTGQKIIYRNGKWQQM"
     gene            complement(3846..4523)
                     /locus_tag="OHN28_003600"
     CDS             complement(3846..4523)
                     /locus_tag="OHN28_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCQ2298925.1"
                     /translation="MLITHIAHKHLNRAVYEKGGDGGASKAQAKAQQQAIDLQREQWN
                     TVMNNLKPYAEVGLPALQQLQGLMTLEGQNKAANDFFGSGLYKTQADQARYQNLVSAE
                     ATGGLGSTATSNQLSSIAPMLYNNWLSGQMQNYGNLLNVGMNAASGQATAGQNYANNT
                     GQLLQGLGAIRAGQAQQPSSLARGIGGAASGALAGAQLGSVVPGIGNVAGAIGGGLIG
                     LVGGLGF"
     gene            complement(4517..4978)
                     /locus_tag="OHN28_003601"
     CDS             complement(4517..4978)
                     /locus_tag="OHN28_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCQ2298926.1"
                     /translation="MELKFIDNPVRLQAFLNEPGNTENIVEPGHTYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYIRCGKPVGVTLYQLTRQQYKEFSEC"
     gene            complement(4995..5156)
                     /locus_tag="OHN28_003602"
     CDS             complement(4995..5156)
                     /locus_tag="OHN28_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298927.1"
                     /translation="MAISVKPVLISEKQMEAIKKIQEEQRKKSGIGVAPTLHEIARGL
                     IDKALAGCM"
     gene            complement(5761..6498)
                     /locus_tag="OHN28_003603"
     CDS             complement(5761..6498)
                     /locus_tag="OHN28_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298928.1"
                     /translation="MNNNYCIPQGMTRTEREELKSFATQCGNAGDIQSLERTLIMIAH
                     WMRQGQRVSFTEYASQWTEAQRERSDGNHSTPEMAKQWPFSGKRCISPGGSDYYPAGV
                     GDEPCCDETEIRHAVTVITAEYPQFNLDGLALHNRNAGWENPLDNPSFIVSAKSCLKW
                     IRDNGMSNAQIESFPQDNPTSDTLKYEVERYNQINHQHSDHPHYIPNGAFIAAMVASG
                     YKVKPAGRMNAFFNISKKGLCAAMGKN"
     gene            complement(6488..6718)
                     /locus_tag="OHN28_003604"
     CDS             complement(6488..6718)
                     /locus_tag="OHN28_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298929.1"
                     /translation="MMSTPFYKVRQLASSSGWQLRFEGRSDWLPIAAWANVEMCIDGD
                     TVEIIIPCVASRDGCIEPTDMAAEIREVKHEQ"
     gene            complement(6895..9246)
                     /locus_tag="OHN28_003605"
     CDS             complement(6895..9246)
                     /locus_tag="OHN28_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCQ2298930.1"
                     /translation="MRNIDLIRQVISASENNWPHVLGCLNINVPDSPRRHAPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKRVNNCDTTEAALLAADVLGIDYRTTETPE
                     ATSQKREQLETERQRREQERLKRAEKDEQQRRDTFSRQFDDMRRKAVNGKSDYLVAKG
                     VGDFTFPVLPDGSLLLALVDKSGAVTAAQTITSHGEKRLLTGSAKRGAYHAINAQKRP
                     HSIIIAEGVATALSCHLIRPDAMTVAAIDAGNLLPVAEVMRRTYPQAQIIIAADNDHQ
                     QGDSESGGINTGKDAAERAAISVAGRVSLPPTDYKADWNDYHQQHGLAAATAAFKDSM
                     YQPRGKGAQVKNHKQSVGALNEISSGEVLSDDEIAVLEEINRTFTHVTIGGKHKVVSL
                     KPSQTGGVSHVFEDLSQFQHYFHHKPRVARKLAGSAWLSWSGKNYKPGGVGFYPVPDK
                     CPDDVFNLYEGLALEPIEGDCTVYLNHLLQVVCAGNEEAYQYLIQWMAHIIQKPDEKP
                     SVAIVMKSVPGTGKGTTVKPLLQILGQYAAHINGAGHISGRFNSILANKLLVFADEVT
                     IHKPSEADRLKAIISEPTFNLERKGIDAEPMPNFARLIFASNSTQVLQAGIRERRYLV
                     LEPSPEKAQSREYFDRLYSWLNDGGAAKLLWHLKGVDLSGFDPQRAPQTDALREEILL
                     GLSGVELFLYGELINEPPFNGEVRLFAKDMVSRFVAWSLERGEKLKEPAARSLLGKSL
                     AQMGLVKHGRPDRGNGVFYELPEVGVLQAAFARLIGMGGYDVF"
     gene            complement(9233..9604)
                     /locus_tag="OHN28_003606"
     CDS             complement(9233..9604)
                     /locus_tag="OHN28_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298931.1"
                     /translation="MRENAEMALSSAIGEQVAKIAGAVWIHNLHSTGEEKMAIQTPEG
                     RTITTSLKPSDVCDLICAFMYPAMRTVHGDKWKLATTAEFDMWLNNDGMLTDYGITKW
                     QMLVSHIANAIDHVGYGDAKH"
     gene            complement(9597..9938)
                     /locus_tag="OHN28_003607"
     CDS             complement(9597..9938)
                     /locus_tag="OHN28_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298932.1"
                     /translation="MITKNFRLNALANKYASALYKHITSTSGGDYFMVDADGEAVRVE
                     IVNGVKGVRSLIDSYTLAAMKVFYPQWETVGIELLDRCVTKDGLTDVGREIWQSMVND
                     IGATVAGGSHA"
     gene            complement(9949..10551)
                     /locus_tag="OHN28_003608"
     CDS             complement(9949..10551)
                     /locus_tag="OHN28_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298933.1"
                     /translation="MNNFLTFHAEATPDGVNIMYRSNDGMTERVEAVSYIDAVNRLDA
                     GDYDDKPDEGMSIHLAIADGGNQGYFDYTSQHNVIMWRWLIATVFMLEMREENGTVSI
                     IDDTGNPSEVAVYSNGIVAMPLYPVAERLAMANNIEGAMIERFGIESGTERAIIFYRA
                     MMDVEQGALTPFGRETLAELHNSFIAELNENGMPAEPVTH"
     gene            complement(10544..10765)
                     /locus_tag="OHN28_003609"
     CDS             complement(10544..10765)
                     /locus_tag="OHN28_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298934.1"
                     /translation="MTNIQLIEAQCRIEQVQTVLGFWLEGASPSNRDKLMIGAVMSLL
                     NGVPEAIQEADELLGKYELQNHSGEAKHE"
     gene            complement(10762..11025)
                     /locus_tag="OHN28_003610"
     CDS             complement(10762..11025)
                     /locus_tag="OHN28_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HCQ2298935.1"
                     /translation="MNINLIYRHPCELEIESLLSREEPYPDTFTLADRTTERLTRART
                     GLVHVMNEILPSVGGEQATVITSWLQKVTSLIDISLIDAESAK"
     gene            complement(11022..11216)
                     /locus_tag="OHN28_003611"
     CDS             complement(11022..11216)
                     /locus_tag="OHN28_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298936.1"
                     /translation="MNNSINTPRLTSALQLIEQVAAVLVAVSLSAEEMDAADVVDAIK
                     ACSSLVNNARAELVILGGEK"
     gene            complement(11209..12237)
                     /locus_tag="OHN28_003612"
     CDS             complement(11209..12237)
                     /locus_tag="OHN28_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCQ2298937.1"
                     /translation="MIPVQKKIFSLAGMSPKSSNMAAKSGINTADTSKVYHLLVVGAD
                     ALTMSEITGDDISVEKVSGCAREFLVVDDLFCSCSDSTKTFVHARDVNEISAMYCASG
                     ASNSEFSESIKKSLPLCGNTVYGYKAPHKTGAGIGLPLTIEAIHDAPASFFVSAHTHT
                     LSMVGCMGLPSGRLVSLIASKANSVQSTASKIGLLGGGYKPSIKEAATCWLLPLPKNR
                     NLSGLSPQFAAIARQLPPKFIILLPSLNAMLAVLWCAITSAFLLVVSAWRWHMIKTYD
                     VHMDPLERTSQIITLTEVINDILVSNSPSRDERLKALLAILDLAVRDVHFLLEGGQMP
                     CKTGATNE"
     gene            complement(12234..12413)
                     /locus_tag="OHN28_003613"
     CDS             complement(12234..12413)
                     /locus_tag="OHN28_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCQ2298938.1"
                     /translation="MSKPTGIPQPQKRYKDAHGALVTVESVSHNRVTFYRDGYQSPCV
                     QPLARFMKEFAEVNQ"
     gene            complement(12406..13239)
                     /locus_tag="OHN28_003614"
     CDS             complement(12406..13239)
                     /locus_tag="OHN28_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCQ2298939.1"
                     /translation="MNIEKSRLISEAAPHLNASLGTINGNEFAAIVPVISGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKHSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRTVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAEQGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEALN
                     V"
     gene            complement(13252..13683)
                     /locus_tag="OHN28_003615"
     CDS             complement(13252..13683)
                     /locus_tag="OHN28_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298940.1"
                     /translation="MEKKNRPLQAANSDIRVSDITPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHVNHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            complement(13683..13901)
                     /locus_tag="OHN28_003616"
     CDS             complement(13683..13901)
                     /locus_tag="OHN28_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCQ2298941.1"
                     /translation="MTAHKPASSAIPTTGYIRRFRMPELLGVSLPTIDRWVKNGTLPR
                     PVKLTDNVTAFDAVEINNWLAERRGKVA"
BASE COUNT         3333 a         3561 c         3093 g         4041 t
ORIGIN      
        1 atgtaccaaa tgccatgtct tggatatatg atgattgcca ttctaaacaa agctaaaggg
       61 agggttggtg tgagggggca ggcagttata attattttgt tgggtttaat tgtcgcaaag
      121 ttatacccga ctttatcgat ggtgtttttt gccattattg gcgcagccat ctttacttat
      181 atcattaaac ataccgcctt gtggttctat aaaattgata taattcagaa gtttgtacgt
      241 cctgtgtcga tgttattact agcattaata ccaattggtg ttgcttatgt aattgatgtt
      301 ttttaccctt cggacactga aaggtggttg atagttttgt attgttatat tggtgtagct
      361 ctcttttgtg gatttcattg gtgtttcgaa aagatcaatg actctatttc aaagaataaa
      421 gattgaggcc atccgtggcc attcactagt cattatctgg atcattagat agccaagtaa
      481 tgatgcccag tctggcgatg gtccgttttt cctgttcagg aagagagtta aaaaaactct
      541 tccattgagg ggagcgcctg atacgtgaat caacaatgtg cccaggcgct tttccgtgta
      601 gattttttac tgcgctctgg aaggctggcg aggcaattaa tttatccgca gcatcagccg
      661 cccctgctcc accagctaat cgagctttac tcgccaatct tgctccaata tcagccccag
      721 caatggctcc aagcgatcca aaatgagcgc ctaccatcgt gcctattttt tgaatgtgtt
      781 ttgctgctag ttcgtatgga gctgttaccc gattaaatct gtttacaaac tcattcagct
      841 taccagtaga tatttcatgc gctttagcat tttttattgc tctggcaact ttgtatacat
      901 catatagtcc gctcattgtt tcttttggca aatgttcagc caaagtcttt agctgtccac
      961 tgtgttgcaa gttttgccac caatctgcaa agccagatgg attaaagtcg gccccacgct
     1021 tcccagagga aagcatatct cttaatgctg ttcctactag ctcccttcgc aatgttcttg
     1081 atggagtatt ttgcataatc tcacgaaacc ctctcgcatc accttttgcc atagattgaa
     1141 gagaattcgt tactttatac gctatatccc cattaagtgt tttcccgcga agatttacca
     1201 tttgcctttc taagtttttt ctcatagcta caaggcgttg tgcgacttcg aaatctcgca
     1261 agtatccagt attggataag acagattttt ggtcttcagc taaagctcca tatagtcttg
     1321 atagcgcttc tttatcagca tctttgtatg gcccctgctt tttatttagg gcatcaccaa
     1381 ctaaacgcct ttgcctgttt aaatttgcat acgttaggat gctatctggg ccggggttta
     1441 ttgctgtaaa aactcgcttt tctattgggt caagattatc ccaccctcct aaatcatcag
     1501 cgattttttc cagcatagtg cttgtgttat ttgcttcgac ttttgttctt ggtgatagag
     1561 cggaatcaac acgtttatat aactgatcac tacggttttt tagtgcttcc attctactat
     1621 ttatctgata aatatatttt tcgctcatag ctaacgcatc tggcgcacca gcaacatcat
     1681 cgattatctt gccagcacta gaggctaatt cgcttattgc tttatcttct tgagctttta
     1741 acgctgatcc tgtccgtgac tttagggctt gctctacagc ttggtattgc tcatttcctg
     1801 ataaatgaga aggaagaact gaatcaacat gaagcctatt tgcagcctca acaatttctt
     1861 tctgaggttt tagatctagc ccttcaagcg aagaggcaag atttgggttg ccttgtgcag
     1921 ataacttacg aattttctct tcggcaggag taatcccctc aatatctgga atcgaagaaa
     1981 cattcttctt ggttaatttc ccagctattt tattaattgc agggccagca agttcggcta
     2041 cagggccagc cacagcgcca atagcagctc cagaagcaac atcgctattt gttccattgg
     2101 ctacaatagc cccttctcca gcaccaagcc ctgcggcggc agccagcctt gccgcccctt
     2161 tcggaacctg agaaataatc ccaccgccac taacaaatgg cgctgcttgt ccaacgaact
     2221 caccaacatc ttgtgcggtt gatggttttg ccgctaactt ctgctgtaga gactgaattg
     2281 cggcttgctc ttctggtgtc atatcctgaa acaggccaac acctttacca acatccatca
     2341 atccactgag aacgccatac ataaaacggt cgaaaccgtt agcattatta acaacatttt
     2401 cctgtctgga attttcctct ggtgaaacca aaggtgattg ctgctgttct ggttgggcat
     2461 caagcacaaa accatcagga agctgtgaac tatcaggaaa ctgtgagtta tcaggttgtt
     2521 catcgagcac aaaaccttcc ggtaagatta catttgctgc cattttccgt tcctgtaaat
     2581 gattttctga ccagttttag agttggttgc cgttgcgcct tctgatattc ctccggttgc
     2641 cgcttttgcc tgtccagtgc cagcctgtga tgtaggctgc ccggaaacat cgtcaaatag
     2701 cttcgctttc cttctttcaa ggctttttct tagcccctga ggaatagttt ctccatatgt
     2761 atcgagatat tcgtcaacct gctggttaaa ctgctgcccc atagcattgg cgttgatttt
     2821 tgcggcgtta acaatgttat cctttgcctc ctgcgacagg ccgtttccag cattaagctg
     2881 gtctatgtat cctttgattt gtccccatat gccatcagac cgcattacct gaacctgttc
     2941 gccttcacga acgactgact gcgggtcaag agatttcatg tagttgaaga taatcccaag
     3001 ttgggcagcg ccagtattta ccttgctaag ggcttgcaga gagtttgccg ccgctctgac
     3061 agagttataa tttttgccaa aattggtaat gtcagaattt aatcccttaa ttaagtctgc
     3121 tgacggctta cctttttgcc ccagctccat tagcttcaat cccatctcat ctgagtgaat
     3181 ttgtgctcgc tgagccctgt ctagttgagc gttctggata tttgcccatc ctctcgcgtt
     3241 ctccatgtca gcctgacgca tactttcatc cagtcgccct tgctcaagtc gacggccttc
     3301 cattcgatct ttgatatcga agtacttctc atgaccgaga gaaaacagtg ccatactacc
     3361 agcaagccgc tggaatccct gtggatcatt aacatgcatt tcggcaacag actcaggaga
     3421 aattcccaag cgacgcattt catcttcgtt atctgctatg aattttccaa atgccccagt
     3481 tccgagcgaa gatgcgattt gagcactagt cgcaagatga ccaatagaat ttctctggtc
     3541 gtcatcaata aagcccatcc ctttctgaac gctttcaaat tcttctggat actcagaaat
     3601 aagactgcgc attttgtttc tgtctccaga ctcaaaagcc ccggcatatg ctttctgaaa
     3661 ctctgcttta cgctgttgtt gacgtgcctg ctgattaatc tcagcaacgc ctcttagccc
     3721 ctgtagcgcc attaaaccta cgtttggctg ctgctgatac tgcacaggct gaggaacgga
     3781 agaacgagga gcattatcat tttgcggtgc catgctaggc agcccgccaa gttgccatgt
     3841 cgccattaga atccaagccc cccaacaaga ccaataagcc caccaccaat agccccggcg
     3901 acattaccta tacccggaac aacggaacca agttgcgcac ccgctaaagc gcctgatgcc
     3961 gccccaccaa taccacgcgc aagactggat ggctgctgcg cctgacctgc acgaatagcg
     4021 ccaagcccct gcaagagttg cccggtatta ttggcgtagt tttgccctgc tgttgcctgc
     4081 cctgatgccg cattcatccc gacatttagc aggtttccat aattttgcat ttgcccactg
     4141 agccaattgt tataaagcat gggcgcaatg gaagataact ggttactggt agctgttgag
     4201 ccaagccccc cggtagcttc tgctgagaca aggttctgat aacgggcctg atctgcctgt
     4261 gttttatata aaccagaacc aaagaaatca ttcgcggctt tattctgccc ttccagagtc
     4321 atcagtcctt gcagttgttg taaagcaggc aatccgactt ctgcatacgg cttgagatta
     4381 ttcatcaccg tattccactg ctcgcgctgc aaatcaattg cctgctgctg ggccttcgcc
     4441 tgagctttag aggcaccacc atcaccgcct ttttcgtata cggcacgatt gagatgttta
     4501 tgagctatat gggtaattaa cattcgctga attccttata ttgttgccga gttaactgat
     4561 aaagcgttac gccgacgggt ttaccacatc tgatataagc atcatcaagg tgaccaacac
     4621 gggtagcgcc aagcaaacgg ataattgccc gtccatattt tgtggtgtca ggaaccatag
     4681 tgatgctgtt aaggaatggt gagttttcga gaagccattt gcagaataat cgatgccctt
     4741 gcagtgcata ttcaccacgg aatccggggt cgtacaccgc atggcattca acaacgctat
     4801 gccagaagtt acgcacttca tgaacgccag ccagcactaa tccttcgtag atgccgagat
     4861 ataccgcatc aggcttgatg tagtacgtgt gccccggctc aacaatattt tcggtattgc
     4921 ctggctcgtt aagaaaggcc tgtaatcgca cagggttatc aataaatttt agttccatgc
     4981 gatgccatcc attttcacat acagcctgcc agcgccttat ctattaaccc tcgtgcaatt
     5041 tcatgcagag ttggcgctac tccaattcct gattttttac gctgctcttc ctgaattttc
     5101 tttatcgctt ccatttgctt ctcacttatc aataccggct ttactgaaat agccataacg
     5161 ccccccctta gaactgtata aaacaacagt tattatagtt tcacaaatcg caatattcat
     5221 catcgtaatt gcaaaatatg aaacaaatag cacaaaaaaa tgccaccgct cagtagctgg
     5281 tggcatcgtt ctgtgtgtca ggttttgaca ggttttttat ggactctaaa aactatattt
     5341 atggtatcgc attactcttc acggcaggtg ctttccagtt ctgcccttgc ttctggctcc
     5401 attggtaatt tgattcgcga tcagctatgc ggatggtttg ctgaacaggc ataacaacat
     5461 ctcagaggct attttttgaa ctacgaaaat ttcgtaggtc tctccttcaa aaacgtcacg
     5521 cccttgtaca ctggatttta caaaagtctc gtaaaactct ttgctgttct gggctatgtg
     5581 ccaaaccgca gcgaatacct ccgtctgcca gatgagcaaa acatacctta taaagaaaat
     5641 cgctgttttt gctataccac ctgtaccacc atgtcacaaa cccttgtgtg gtgcggtctt
     5701 caggtggtac aggtgggtaa aaatgacctc tccccacctg tacagacctg taccatttat
     5761 ttaattttta cccatagcag cacataatcc cttttttgaa atattgaaaa atgcgttcat
     5821 tcttcccgct ggcttaacct tgtagccgct tgccaccatc gccgcaataa atgctccgtt
     5881 ggggatatag tgcgggtgat cactgtgctg gtggtttatc tggttatatc gctccacttc
     5941 atacttcaac gtgtcagatg tggggttatc ctgcgggaag ctctcaatct gggcattact
     6001 catcccgtta tctctgatcc atttcaggca gcttttcgcc gatacgataa atgacgggtt
     6061 atcaagcggg ttttcccagc ccgcattccg gttgtggagc gccagtccgt caagattaaa
     6121 ttgtgggtat tcagcggtaa ttactgtcac tgcgtggcgg atttcagtct cgtcacaaca
     6181 tggctcatct cccacaccag cagggtaata atctgaacca ccgggactga tacagcgttt
     6241 accactgaaa ggccattgct tcgccatctc aggcgttgag tgattaccgt cgctccgttc
     6301 gcgctgtgcc tctgtccact ggctggcata ttcagtaaat gaaacccttt gcccctgacg
     6361 catccagtgc gcaatcataa ttaaagtgcg ttccagactt tggatgtcgc cagcattccc
     6421 gcactgtgta gcgaaacttt ttaattcttc gcgctccgtt ctggtcattc cctgcggtat
     6481 gcaatagtta ttgttcatgt tttacctctc tgatttctgc tgccatatct gttggctcta
     6541 tacagccgtc ccgacttgcc acgcagggaa taatgatttc aacggtatcg ccgtcaatac
     6601 acatttcaac attcgcccat gccgcaatgg gtagccagtc tgaacgcccc tcaaaccgta
     6661 gctgccagcc agaggaagaa gctaactggc gcactttgta aaatggtgtg ctcatcattg
     6721 gttgttccct ctcagccagc cagaagaaat gcgtttgtta atggtatagg tggtacaggt
     6781 ctggtatagg taagatattg acctgtacca ctataacaca ttgtttttaa tgctgaatta
     6841 gcaaagtggt ataggtggta caggtaaaaa cgatcttctt gtaaaaagac tatattaaaa
     6901 aacatcataa ccccccatac cgatcagacg ggcaaacgca gcctgtagaa ctccgacctc
     6961 tggtagttcg tagaacacgc cattccctcg gtctggtctt ccatgcttca ccagccccat
     7021 ttgtgcaagt gatttaccca gtagtgatct ggcagctggt tctttaagct tttccccacg
     7081 ctcaagcgac cacgctacaa accgactaac catatccttt gcaaacaaac ggacttcgcc
     7141 attaaacgga ggttcattga ttagctcgcc ataaagaaac aattcaacgc cagacagccc
     7201 cagcaagatt tcttcacgta aggcatcggt ctgtggcgct cgttgagggt caaaacctga
     7261 gagatccacc cctttaagat gccaaagcaa ctttgcggca ccgccatcat taagccaact
     7321 gtacagccga tcaaaatact cccggctctg tgctttttca ggagatggct caagcacgag
     7381 atagcgacgc tctcttatcc ctgcctgtaa tacctgtgtg ctgttactgg caaatatcaa
     7441 ccgggcaaaa ttcggcattg gttcagcatc aattcccttg cgctcaaggt taaacgtcgg
     7501 ttcgctaata atcgctttaa gtctgtcagc ttcagacggc ttgtggatcg tcacttcgtc
     7561 agcaaatacc agtagcttgt tagcaagtat tgaattgaag cgccctgaaa tatgtcccgc
     7621 cccgttaatg tgggcggcgt actgccccag tatttgcagc agcggtttaa ccgttgtgcc
     7681 tttccctgtg cccgggacag atttcatcac gattgccacg gacggttttt catcaggctt
     7741 ttggataatg tgcgccatcc actggataag atattggtat gcctcttcat taccggcaca
     7801 gacaacctgc aacaggtgat taaggtatac cgtgcaatct ccttcaattg gttccagtgc
     7861 cagcccctca tacagattaa aaacatcgtc agggcatttg tctggtactg gataaaatcc
     7921 tacgcctcct ggcttgtagt tcttcccact ccatgacagc cacgccgatc ccgccagctt
     7981 tctggcgact ctcggtttat gatgaaaata atgctgaaat tgtgataaat cctcgaaaac
     8041 gtgcgataca ccgccggttt gagaaggctt tagcgacacc accttgtgtt tcccgccgat
     8101 ggtgacatgc gtaaacgtcc ggttgatttc ttcgaggaca gcaatttcat catcgcttaa
     8161 cacctcgcca gaactgatct cattcagcgc cccgactgac tgtttgtgat ttttcacctg
     8221 cgcccctttc ccccgtggct ggtacatcga atctttaaat gctgctgtgg ctgccgccag
     8281 cccgtgttgt tggtgatagt cgttccagtc ggctttatag tcagtcggtg gcagagacac
     8341 cctgccagct acggaaatag cggccctctc tgcggcatct ttccccgtgt tgatccctcc
     8401 actttctgag tctccttgct ggtgatcgtt atctgcggca atgattattt gtgcctgcgg
     8461 atatgttctg cgcatgactt ccgctactgg caacaggttg ccagcgtcga ttgctgccac
     8521 tgtcattgcg tcagggcgaa ttaaatggca cgacagggcg gtagccaccc cctcagcaat
     8581 tatgatgctg tgaggtcgtt tctgtgcgtt tatggcgtga tatgcccccc gctttgccga
     8641 acctgtcagg agtctttttt caccatgtga ggtaatagtc tgtgctgctg tgactgcgcc
     8701 ggatttatcc accagcgcca gcaacagaga tccatcgggc aacacgggga atgtaaaatc
     8761 acctacccct ttcgcaacca gataatcaga tttgccgttt acagcctttc tgcgcatgtc
     8821 atcaaactga cgggaaaacg tatcccgtct ttgttgttcg tccttctctg cccttttcag
     8881 gcgctcctgt tcgcgtcgct ggcgctcggt ttccagttgt tcccgtttct ggctggtggc
     8941 ttctggtgtt tccgttgtcc ggtaatcaat acccagaaca tcagcggcaa gaagcgccgc
     9001 ctctgttgtg tcgcagttat ttacgcgttt aattaaatcc agcccatcac cagcgccgca
     9061 ctgattacag atgaagctac cgcgcccgtt gtcatcgaac cggaatcgat ctttgccccc
     9121 acatgcaggg cagggagcat gacggcgcgg agagtcaggg acatttatgt tcaggcagcc
     9181 cagcacatga ggccagttgt tttcagacgc actgataacc tgacggataa gatcaatgtt
     9241 tcgcatcacc gtaccccacg tggtctatag cgtttgcgat atggctaacc agcatctgcc
     9301 acttggttat tccgtaatca gtcagcatgc catcattgtt tagccacata tcaaattcag
     9361 cggttgtcgc aagtttccac ttatcgccgt ggacagtcct cattgctggg tacatgaatg
     9421 cgcaaatcag atcacaaaca tctgacggct tcagagatgt agtaatggtg cgtccctctg
     9481 gcgtctgaat tgccattttc tcttcaccag tagaatgaag gttatgaatc cacacagcac
     9541 cagcaatctt tgccacctgc tctccgattg cggagctaag cgccatttct gcattttcac
     9601 gcatgagaac ctcccgctac agttgcgcca atgtcattaa ccatgctttg ccatatctca
     9661 cgcccgacgt cagtcaatcc gtccttagtg acgcaacgat caagaagctc gatccccacg
     9721 gtttcccact gcgggtagaa cactttcatg gctgccagtg tgtagctatc gatcaggctg
     9781 cgaacgcctt ttacaccatt gacgatttcc acacggacgg cctcgccgtc cgcatcaacc
     9841 atgaaatagt ccccgccgct ggtggaggta atgtgtttat acagtgccga cgcatactta
     9901 tttgccagcg cattcagtcg gaaattttta gtaatcatgg ctccatcctc agtgcgtcac
     9961 tggttccgct ggcatgccgt tttcattcag ttcagctata aagctgttgt gaagttcagc
    10021 aagcgtttca cgaccaaatg gagtcagtgc tccttgttcc acatccatca tggctctgta
    10081 gaaaatgatg gcgcgttctg tgccagactc gatgccaaaa cgttctatca tcgcgccctc
    10141 aatgttgttt gccatagcga ggcgctctgc tactggatac agcggcatgg cgacgatgcc
    10201 gttggaataa acagccactt cggaaggatt gccagtatca tcaatgatgc tgacagtgcc
    10261 gttttcctct ctcatttcca gcatgaatac tgttgcgatc agccatctcc acataatgac
    10321 gttatgctgt gatgtgtaat cgaaatatcc ctggttgccg ccatcggcaa tagcgagatg
    10381 tattgacata ccttcatctg gtttatcgtc ataatccccg gcatccagac gatttacggc
    10441 atcaatatat gaaacggcct caacgcgttc tgtcatgcca tcgttgctgc ggtacatgat
    10501 gtttacgccg tcaggcgttg cttctgcatg gaaagttaag aaattattca tgtttcgcct
    10561 cgcctgaatg attctgtaac tcatatttgc ccaacaattc gtctgcttcc tgaatagctt
    10621 ctggtacgcc attgagcagt gacataaccg cgccaatcat taacttgtct ctgttgctgg
    10681 ggctggcccc ttcaagccag aaccctaaaa cagtctgaac ctgttcgata cgacattgcg
    10741 cttcaatgag ctggatgttg gtcatttcgc actctccgca tcgattaaac tgatatctat
    10801 cagagaggta actttttgta gccagctagt gattactgtc gcctgttccc ctcccaccga
    10861 tggaagaatc tcattcatca catgaaccag acctgtgcgc gctctggtaa gccgttcagt
    10921 cgtgcgatct gcaagagtga atgtgtctgg atatggctct tcacggctca gcaatgattc
    10981 aatttccagc tcacacggat gacgataaat taagttgata ttcatttttc acccccaaga
    11041 attaccagct ctgcacgggc attgttaacc aaagatgagc acgctttaat cgcatccacg
    11101 acatcagcag catccatttc ttcagccgag agactgacag caaccaggac agctgctact
    11161 tgctcgatta attgaagtgc ggacgtaagg cgaggggtat taattgagtt attcattggt
    11221 tgcccccgtt ttgcatggca tttgtccacc ttccagcagg aaatgaacgt cacgaacggc
    11281 gaggtccaat atcgcgagta acgccttcag tctttcgtct cgtgagggag agttgctcac
    11341 cagaatgtcg ttaatcactt ctgtcagtgt gatgatctgg cttgtgcgtt cgagggggtc
    11401 catatgcaca tcgtaggttt taatcatgtg ccacctccat gcggatacga ccagcaaaaa
    11461 agcagatgtg atcgcgcacc agagaacggc gagcatcgcg ttcagactcg gcagcaatat
    11521 gatgaatttt ggcggtaatt gtcgggcaat cgcggcgaac tgcggcgata atccagataa
    11581 attgcggttt ttgggtagtg gtagtagcca gcatgtggca gcctccttga ttgatggttt
    11641 atagccaccg cccaagagac caatcttgct ggcggtggac tgtacggagt tggccttact
    11701 ggcaatcaag gaaaccagcc tacccgaagg tagccccata cagcccacca ttgataaggt
    11761 gtgcgtgtgc gccgatacaa aaaaagacgc tggcgcgtca tgtatcgcct cgattgtcag
    11821 cgggaggcca atcccggcac ccgttttatg aggtgcctta taaccataaa ccgtgttgcc
    11881 gcataacggc aagctctttt ttatggactc cgaaaactca ctattggaag caccagatgc
    11941 acaatacatc gcgcttattt cgttaacgtc acgcgcgtgg acgaaggttt ttgtggagtc
    12001 actacacgag cagaacaggt catctacgac cagaaattct ctggcgcagc cgctgacctt
    12061 ctcaacgcta atatcatcgc ccgtgatttc tgacatagtt aaggcatccg ctcctaccac
    12121 cagcaaatga taaactttgc ttgtatctgc tgtattaatg cctgactttg ccgccatatt
    12181 gctggatttt ggcgacatgc cagccagtga aaatatcttc ttctggacgg gtatcattgg
    12241 ttaacctccg caaactcctt catgaaacgc gccagcggtt gtacgcatgg cgattgatac
    12301 ccgtcgcgat aaaacgtcac tcggttgtga gacacgcttt cgacagtaac gagtgctccg
    12361 tgggcatctt tgtagcgttt ttgtggttgt ggtattcctg ttggcttaga cattgagcgc
    12421 ctccagacgc ttggcgagcc agcgttgcga caggcgggtg agttctcctt tgcgctgctc
    12481 atattccatg cccatatcaa ttaacgtgat attggtgctt tcgagatatg agaggtgctc
    12541 aagctgttct gcgttcatat ggtcgcgagg ttcgccagaa tagccattta tccgcgccca
    12601 ctgcttagca gttagcccac caagaacgat acgagaaatc atattgctct cgtttgtgta
    12661 gtggtgttgc tgcgtcgttt tcccctgctc ggcacgggcc atgttcagcg catcgcacat
    12721 tggcttaaag ttgtttgcgg cactgatacg ggcttttagc tggcgacgat agcgggcggc
    12781 gatttcaggc acggtacgct gtaattcttc ctcgcactgg atgaaataac gacggatagc
    12841 gcgaccctga tcattgcgtt cgatcattgc cagttctttc gccattccga cacttaacag
    12901 atagtctttg ccgggtctgc cagagtgctt aatcttgctg taagccgcgc cattcgggct
    12961 tttccccaaa tttggggaaa ttgttttatg gactgagtaa tcgtgcccaa tggtgaagtc
    13021 atattcagag atgcgatcag tgatccatgt agagaagtct tttcccacgc ccaacgcttt
    13081 gtgtaacgct tttgcgctaa caatattggt ttcacgccca cctatatgac cagaaataac
    13141 cgggacaatt gcggcaaatt cattaccgtt aattgtgccc agagaggcgt tcagatgagg
    13201 ggcggcctca gaaattaatc tgcttttttc gatgttcatt tttagtctcc gttaggcggc
    13261 gttaaacgcg tccgggtaga gtttcagaat gtcggcaatt tcctgctgag aaagtccgtt
    13321 gtgatcattt atgttcgcaa gatggttaac atgagtgatc actttcagaa catcaccacg
    13381 gcaggagaaa cggtagcgaa ggtgtgttcc gataccatca ggattctttt cgtcgatacg
    13441 ctccagagtg attccaagct ggcgttctaa ctctgttgca tagttacgac cagaagacag
    13501 acggcagtag cgaagaatgt cattttctgt ccatccctct ataccagtac gcagcatata
    13561 gactcgggca cgatgctttt tcggtgtgcg ctttggtgct tgaagggatt ttgtaagggg
    13621 cgtaatatca gatacgcgaa tatctgaatt agccgcctgt aatgggcggt ttttcttttc
    13681 catcaggcca ccttcccgcg gcgttctgcc agccagttat taatttccac cgcatcaaac
    13741 gcagtcacgt tatctgttag cttcactggg cgtggaagag tgccattttt aacccatcta
    13801 tcaatagtag gcagtgaaac acctagcaat tccggcatgc gaaagcgacg gatgtatcca
    13861 gttgttggga tagctgatga tgctggcttg tgtgctgtca tatctctagt aacctctctg
    13921 atagctcatg aagttactta aagtttagtg gttgcccata cagtatttct attaagacta
    13981 gaatctattg aaagaaatta taaatctatt gaaaaaatac cgatccac
//
LOCUS       DAJOTB010000122        13804 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJOTB010000122 DAJOTB010000000
VERSION     DAJOTB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.122"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..251
                     /locus_tag="OHN28_003617"
     CDS             <1..251
                     /locus_tag="OHN28_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCQ2298942.1"
                     /translation="VALDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDE
                     EHKNTMCKDGRCPDINKKVQTGDGINNDVDTKTDGTTQ"
     gene            365..1306
                     /gene="zitB"
                     /locus_tag="OHN28_003618"
     CDS             365..1306
                     /gene="zitB"
                     /locus_tag="OHN28_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCQ2298943.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(1303..2022)
                     /gene="pnuC"
                     /locus_tag="OHN28_003619"
     CDS             complement(1303..2022)
                     /gene="pnuC"
                     /locus_tag="OHN28_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCQ2298944.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(2060..3103)
                     /gene="nadA"
                     /locus_tag="OHN28_003620"
     CDS             complement(2060..3103)
                     /gene="nadA"
                     /locus_tag="OHN28_003620"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCQ2298945.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(3381..3456)
                     /locus_tag="OHN28_003621"
     tRNA            complement(3381..3456)
                     /locus_tag="OHN28_003621"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:Lys,seq:ttt)
     gene            complement(3490..3565)
                     /locus_tag="OHN28_003622"
     tRNA            complement(3490..3565)
                     /locus_tag="OHN28_003622"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3530..3532),aa:Lys,seq:ttt)
     gene            complement(3614..3689)
                     /locus_tag="OHN28_003623"
     tRNA            complement(3614..3689)
                     /locus_tag="OHN28_003623"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3654..3656),aa:Lys,seq:ttt)
     gene            complement(3693..3768)
                     /locus_tag="OHN28_003624"
     tRNA            complement(3693..3768)
                     /locus_tag="OHN28_003624"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3733..3735),aa:Val,seq:tac)
     gene            complement(3820..3895)
                     /locus_tag="OHN28_003625"
     tRNA            complement(3820..3895)
                     /locus_tag="OHN28_003625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3860..3862),aa:Lys,seq:ttt)
     gene            complement(3898..3973)
                     /locus_tag="OHN28_003626"
     tRNA            complement(3898..3973)
                     /locus_tag="OHN28_003626"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3938..3940),aa:Val,seq:tac)
     gene            complement(4009..4084)
                     /locus_tag="OHN28_003627"
     tRNA            complement(4009..4084)
                     /locus_tag="OHN28_003627"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4049..4051),aa:Lys,seq:ttt)
     gene            complement(4249..5040)
                     /gene="cpoB"
                     /locus_tag="OHN28_003628"
     CDS             complement(4249..5040)
                     /gene="cpoB"
                     /locus_tag="OHN28_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCQ2298946.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTSNAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(5050..5571)
                     /gene="pal"
                     /locus_tag="OHN28_003629"
     CDS             complement(5050..5571)
                     /gene="pal"
                     /locus_tag="OHN28_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCQ2298947.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(5606..6898)
                     /gene="tolB"
                     /locus_tag="OHN28_003630"
     CDS             complement(5606..6898)
                     /gene="tolB"
                     /locus_tag="OHN28_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCQ2298948.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(7031..8341)
                     /gene="tolA"
                     /locus_tag="OHN28_003631"
     CDS             complement(7031..8341)
                     /gene="tolA"
                     /locus_tag="OHN28_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCQ2298949.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAAD
                     KKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNA
                     SPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLD
                     IKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(8406..8834)
                     /gene="tolR"
                     /locus_tag="OHN28_003632"
     CDS             complement(8406..8834)
                     /gene="tolR"
                     /locus_tag="OHN28_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCQ2298950.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(8838..9530)
                     /gene="tolQ"
                     /locus_tag="OHN28_003633"
     CDS             complement(8838..9530)
                     /gene="tolQ"
                     /locus_tag="OHN28_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCQ2298951.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(9527..9931)
                     /gene="ybgC"
                     /locus_tag="OHN28_003634"
     CDS             complement(9527..9931)
                     /gene="ybgC"
                     /locus_tag="OHN28_003634"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2298952.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(10081..10374)
                     /gene="ybgE"
                     /locus_tag="OHN28_003635"
     CDS             complement(10081..10374)
                     /gene="ybgE"
                     /locus_tag="OHN28_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCQ2298953.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGFFCPLLADIVLIVGLIFFFF"
     gene            complement(10374..10487)
                     /gene="cydX"
                     /locus_tag="OHN28_003636"
     CDS             complement(10374..10487)
                     /gene="cydX"
                     /locus_tag="OHN28_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCQ2298954.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(10502..11641)
                     /gene="cydB"
                     /locus_tag="OHN28_003637"
     CDS             complement(10502..11641)
                     /gene="cydB"
                     /locus_tag="OHN28_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2298955.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(11657..13225)
                     /gene="cydA"
                     /locus_tag="OHN28_003638"
     CDS             complement(11657..13225)
                     /gene="cydA"
                     /locus_tag="OHN28_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCQ2298956.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         3474 a         3662 c         3247 g         3421 t
ORIGIN      
        1 aggtagccct tgatgcccgt gaaaatgtcg cgccaaataa cgtcgacaat aacggggtaa
       61 ataccggttc tggcggcaca atgctgcatc cggatggttc ttcaatgaac aatgacggca
      121 tgaccaaaga tgaagagcac aagaacacca tgtgcaaaga tggtcgctgc ccggatatca
      181 ataaaaaagt ccaaaccggt gatggcatca acaatgatgt cgataccaaa accgacggta
      241 ccacgcagta accctgcgaa atagccaata atgggagagc gtagtctctc ccttttaatt
      301 tttcccttct aaaagcagta tgatgtcata aagttaagtg agaataatta agggatgact
      361 cgtcatggcg cactcacact cacacacttc ctcacacctg ccagaagata ataatgctcg
      421 tcgcttgttg tatgctttcg gcgtgactgc cgggtttatg ctcgtagaag tcattggtgg
      481 ttttctttcc ggttctctgg cattgctggc cgatgcgggt catatgttga ccgatactgc
      541 cgccctgctt ttcgccctac tcgcagtgca attttcccgt cgtcctccca ccattcgcca
      601 cactttcggt tggctaagat tgaccacgct cgcggcgttt gtgaatgcca tcgccctggt
      661 ggtgattact attttgattg tctgggaggc gatagaacgc ttccgcactc cgcgtccggt
      721 cgagggcggc atgatgatgg caattgccgt ggctgggctg ctggcaaata tactttcttt
      781 ctggttactt catcacggca gcgaagagaa aaacctcaac gtgcgagcag cggcactgca
      841 tgtgctcggg gacctgctgg gttcggttgg ggctattatt gccgcgttga ttattatctg
      901 gaccggctgg acacctgctg accccattct ctcaatactg gtgtcgcttc tggttctgcg
      961 tagcgcgtgg cgactgttga aagatagtgt gaatgaatta cttgaaggtg caccggtatc
     1021 gctggatatc gctgaactga agcgtcgctt gtgccgggaa atcccggaag tccgcaatgt
     1081 gcaccatgtg catgtatgga tggtaggcga gaagccggtg atgacgctgc atgtgcaggt
     1141 gatcccgcca cacgatcacg atgccttgtt ggatcagatc caacactacc tgatggatca
     1201 ctatcagatt gagcacgcca ccattcagat ggaatatcaa ccttgtcatg ggccagactg
     1261 ccacctgaat agaggggtgt ctggccattc acatcatcat cattaatggg acagcgcacg
     1321 tgaacctctt tcgcgtgcgc ttttgatcca catccggctg ccgttgaggg caataaaggt
     1381 caggatgatg tactccagag acatggcgta aacgccctga agtgcaaaaa taacgacgct
     1441 aatcacgtta ataatcaccc acaacagcca gttttccaca tacttacgtg tcatcagaat
     1501 cattgccacg atagataaca ccatcatgca tgaatcccag aacgggaaag cgtccggctg
     1561 tagttcaggc atcgcaacct gtaatcctaa tgcttgcatg atcatgactg ccacgcgggt
     1621 caaaaatgca aacacaggat tgataaatac cgtcattaga ccaatcgaaa caacgcaaac
     1681 cgccagccag cttagcgcct tcggcaatgg caaccagcga attttcaact ccgcctcgtt
     1741 ctgactggtt tgtcgcgacc acgcatacca gccgtaaata ttcgcggcaa agaaaaacac
     1801 ctgtaataac aggctggcat acagctgaat ctgaaagaaa ataatgccaa acaaggtgac
     1861 gttgatcagg ccaaagaagt agttactgat cttctccaga ctggcaaggc caatacacag
     1921 caacccggcg atcgtgccta ccgcttcgat ccatgagaga tcataaccgc ctgcccctat
     1981 tggtatatgt accaggatat tctgcacact aaaaaaatcc atcgttcccc caaagcgtaa
     2041 tgtgacgcct tattatcaat tatccacgta gtgtagccgc aaaatccagc atacgattga
     2101 gcggcaccag cgccctctct cgcagccttt catcaacata aacctcgtga ttgcttcctt
     2161 ccagttctaa tgcctctgcg atggcctgaa ggccattcat ggccatccac ggacaatgcg
     2221 cgcagctgcg acaggttgcg ccctcacccg cggttggtgc ttccagtaac tctttatctg
     2281 gcaccgcctg ctgcattttg tagaaaatgc cccgatcggt tgccacgata agccgctgat
     2341 gttgcaatgt tttcgcagca gcgatcagtt gactggtgga accgaccgca tccgccatct
     2401 ctacaatagc ttgcggtgat tctggatgca ccagtatggc agcatccggg tattcttctt
     2461 gcaagcgggt taacgcctga gtcttaaact catcatgcac aatgcaggca ccctgccagc
     2521 ataaaatgtc tgcgcccgtc tgtttttgca cgtaacgccc cagatgtttg tcaggtgccc
     2581 agatgatttt ttcacccaaa ctatccagat gatcaataag ttcgacggca atgcttgaag
     2641 ttaccaccca atctgcgcgc gcttttaccg cagcagaagt gttggcgtag acgacgacag
     2701 tacgatcggg atgggcatcg caaaatgcgt taaattcttc aacagggcag ccgagatcca
     2761 gtgaacattc agcctgaagt gtcggcatca gaattgtttt ttccggactg agaattttgg
     2821 cggtttctcc cataaatctc accccagcga ctagcaacgt agaagcggga tgctttgcac
     2881 cgaagcgcgc catttccaga gaatcagaaa tacagccacc ggtttcttct gccagttgtt
     2941 gaatttcggg atcggtatag tagtgggcaa ccatcaccgc attacgttct tttagtagac
     3001 gttttatctt ctcgcggtaa ttcgcttttt catcaatgct taacggcgtc ggcttcgggg
     3061 ggaaaggata aatcgccgtg tctggatcaa acatcacgct catcttacca tctcgttcta
     3121 caggcttaac gttaaaaccg acttaagcgt gatattcgct attatcggcg taatttgttt
     3181 tatatactaa acaagatagt cgaatggtgg ggaaaagtca ctcgaattac gtatgagatg
     3241 ccgaatgcca cactttgcgt cttattcctc cgacgtagag ttgtttacgc agcaaacgcg
     3301 gagtatgtca tcagggtcaa gaattgaaat tgagagatgc ctgaatagca aaaaaacgcc
     3361 tttagggcgc ttttttgcat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
     3421 aaaagtcaac tgctctacca actgagctaa cgaccccttg cgggattatg ctgctttaat
     3481 cattcaggat ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
     3541 gctctaccaa ctgagctaac gacccatacg ggtgttacct gaaagatttc tactcggaac
     3601 caatgtttaa aattggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
     3661 aactgctcta ccaactgagc taacgacccg agtggtgggt gatgacggga tcgaaccgcc
     3721 gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccga tactgcgctg
     3781 gatactacaa actacttcca ccgaacccac cgtaatcagt ggtgggtcgt gcaggattcg
     3841 aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac gacccggtgg
     3901 tgggtgatga cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct
     3961 gagctaatca cccgatacta cgctggatac tacaactaac tatgttagtg gtgggtcgtg
     4021 caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg
     4081 acccactttt tcgttgcttt cggtttgttt gatatcccgt ggcaacggcg gcatatatta
     4141 ctgatttcag acttgagcgc aacaaaaatt tcgatgtaga tcactcaact gcttatgatt
     4201 cgcacgacac gaccagaaat aatgcgactt ctggtcgtgt gttatgcatt acatcgcgtt
     4261 cagacgtttt tgcgcctgtt tagcgccatc agtaccaggg tatttactga taacctgctg
     4321 gtatacggct ttcgcttttg cggtgtcacc tttgtcctgc atgatgacgc cgactttaaa
     4381 catcgcatct gcagccttcg gtgacttcgg atagtttttc actaccgaag caaaataata
     4441 cgccgcatca tcttttttac ccttgttgta gtttaactga cccagccaat aattggcgtt
     4501 tggcaggtaa gttgaatcag ggtaattttt gatgaaattc tgaaatgcca ccattgcgtc
     4561 atcctggcgg gatttatcct gcaccagcgc aatagctgca ttgtaatccg tgtttgcatc
     4621 accgcttttc accggcgcgc cagcattcga agtaccagca tcagctgtcg gcgtcgttga
     4681 tgccaccaca ccgctttgat cgccgctggt tgattgcgcc gctgcaccac cgctgctgag
     4741 gctgtcgatc tgcaacagga tctgcttttg ccgctccacg acctgattca gttgatactg
     4801 attttcctga atctgaccac gcagggaatc aatatcggat tgattatcag agagttgttg
     4861 ctggagttgg gttaaaagct ggctgtgagc attagaaata cgctcaagtt gagtgacgcg
     4921 gtcttcgacc gagcctgagc cgacactact gattggtgcc tgagcaaaag cggcccaggg
     4981 ggccgctata ccaaccagta acgacagact caatagttga tgtctgaagt tactgctcat
     5041 gcaattctct tagtaaacca gtaccgcacg acggtttttg gcatacgccg cttcgtcatg
     5101 acccagtact gcaggttttt ctttaccgta agaaacgatg gagatctggt cagcagaaac
     5161 gcctttaccc tgcagataca tcttaacagc gttcgcacga cgttcaccca gggagatgtt
     5221 gtattccgga gtaccacgtt cgtccgcgtg accttctacg gtgactttgt aagacgggtt
     5281 gctacgcagg aagtttgcgt gtgcatccag catttgagcg aagtcagaac ggatatcgta
     5341 cttgtccaga tcgaagtaaa cgatgttgtt ctgctgcagc tgttgcattt gcagacgagc
     5401 ctgctcttcg gaagacatgt tgccgttgcc gccgttcgca tccataccag tgccggcacc
     5461 cagcatgcct tcgctgccgt cattgctggc gttcttgttg gaagaacatg ccgcaattgc
     5521 cataacaggc agagcaatca tcagcccttt cagcactttg ttcagttgca tttcaatgat
     5581 tcctttacta ttcaattaat tattatcaca gatacggcga ccaggcaggg aatttgacct
     5641 gtccatcagt tgccggaaga cgcgctttga aacgcccatc tgtagaaacc aaattcagca
     5701 cggatcccat cccctgagaa gagctgtaga ttaccatagt gccgttaggt gccagacttg
     5761 gcgtttcatc caggaacgtg gacgacagaa cttgtacgcc tcccgttgcc agatcttgtt
     5821 tggcaatgtg ctgctgccca ccgttggagc tgaccattac cataaattta ccgtcgctgc
     5881 tgacatccgc atcctggttc tgcgaacctt cccaggtaat acgttgtggc gcaccgccgt
     5941 tgatattcac tttataaacc tgcggacgac cggcctggtc agaagtaaat gccaggttct
     6001 ggctgtccgg gaaccaagtc ggttcggtat tgttactgcg accatcagtt acctggcgga
     6061 tctgaccaga agccaaatcc attacgtaca ggttcaggct accggttttc gacaaggcga
     6121 atgccagttt gctgccgtct ggcgagaatg caggtgcacc gttgtgacgc gggaatgaag
     6181 ccacctgacg tacagcgcca tttgccagcg tctgaataac cagcgcggaa cgaccgcttt
     6241 cgaaggtcac ataagccagt ttagaaccgt ctggtgacca cgccggagac atcagcggtt
     6301 gcggtgaacg gtgaacgaca aactggttgt aaccgtcata gtcagatacg cgcagttcat
     6361 acgggaactg accgccgtta gtctgaacaa cgtaggcaat acgggtacgg aacgcacctt
     6421 taataccggt cagtttttca aatacttcgt cactggcggt atgaccagca taacgcagcc
     6481 actgcttgtt cactttgtac gagttctgag caagtacagt acccggtgcg ccgccagtgt
     6541 caacaagttg ataagcaaca ttgtaagaac catccggatt cggagtgacc tgaccgacaa
     6601 ccacagcgtc aatgcccagc gcggaccatg cagctggttg tacttcctgc gcactacccg
     6661 gctgctgtgg cagacgagca cgatctaacg gattaaattt accgctgtta cgcaagtcag
     6721 cagcaacgat gccgccaata tcttcaggtg ccgcaccagg ccccgcccac tggaaaggaa
     6781 caacaccaat aggacgaccg gaatctacac cgctgtcgat cacaatgcgg acttcagcat
     6841 gcagaactga tgcccacagt atgagaaaac caaatgctac tcgtaatgcc tgcttcatca
     6901 tatctccctt atctggaccg atggcccacg ataatttagc agaatgttaa caaactaaaa
     6961 tacacaaaac taccagaacc ccgtggcaac cggtgcctga tgttgaccgt cggaacagtc
     7021 aacatcgcga ttacggtttg aagtccaatg gcgcgttttt gaacacttca tatactgcct
     7081 ggcttggtgg tttcgggatc ttcgcaagtt tagctgctgc caacgcagct tgacaaagtg
     7141 cgggatcgcc accttcaggt ttgatatcca gtaacatacc atcgggtgcc agttttatgc
     7201 gcagcgtaca ggttttgcct gcataggacg atgcgtcata gaacttactt tcgatagcag
     7261 atttaatctg cccggcatag ttattgatat cggcccctga tgcgccattg tttttagtat
     7321 taccactccc ggcaggcgaa gcattgttcc ctttcgcccc tccccccgtt ttcggtgcat
     7381 tcttaccaga gcttagctca ccgaaaatat catctgcctc agcggcagct tttgctgcag
     7441 cggctttttc agctgctgct tttgccgctg ccgctttttt gtctgcggcg gctttttctg
     7501 ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt
     7561 cggctgccgc tttctctgct gccgctttct tatcagctgc agccttttca gcagccgctt
     7621 tttctgctgc ttttttgtcg gctgccgctt tctctgctgc cgctttcttc tcagcttctg
     7681 ccttggcttt ttcagcagct tcggttgccg ctttctttct tgcttcagct gcagctgctt
     7741 ctgccgcttc agctttcttc ttcagcgccg cagcagcagc ctcggctttt ttctgcgctt
     7801 cgactgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgcc gccgctttct
     7861 tcgctgcttc ttccgcagct ttagcatccg cttcggcctt cgctttagca tctgccgccg
     7921 ctttcgctgc cgcctcttcc gcttgcttct gctttaactc ggcctgtttt gcggcttctt
     7981 cagcctgctt tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt
     8041 cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgctct ttcatcttgc
     8101 gctgctcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa
     8161 ctaccgcacc tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg
     8221 aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca
     8281 gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca
     8341 ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca
     8401 gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag
     8461 ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc
     8521 gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa
     8581 cgctccaggc gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca
     8641 atcactggcg gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg
     8701 acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc
     8761 agtacgtcca gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca
     8821 cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc
     8881 agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc
     8941 tggttgagac ggttataagc cattaccgcc ggaattgcgg cgaacaaacc aattgccgtc
     9001 gcaatcaacg cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc
     9061 ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg
     9121 ctgatggagc caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacga
     9181 ttcatggaaa tacgcatcgc acgcgacgcc ccttccacta cggcttccgg cgcatggcta
     9241 ttggcacgat gcaggcgcac aaactctttg aagccgctgt aaaagatttg ttccgaaccg
     9301 ctcagattat cccgtttccc ctggctctct tgatagaggc gagagagttc gattccagac
     9361 cagaatttat cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat acgggtccgc
     9421 tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta
     9481 accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact
     9541 ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa
     9601 ccagaacctc tgcttcattc aacaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa
     9661 ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc
     9721 gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca
     9781 tcagcgcctg ttgactgaaa tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa
     9841 aagcgacgta actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc
     9901 gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta
     9961 gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta
    10021 agggagcgta aaaatttatg ggcctgactc ggcccataaa tttttaaaga gaattctgat
    10081 ttaaaagaag aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa
    10141 aaacccttgc cagagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca
    10201 gacggcccac ataagcagta aaccatgcca gatttccagt tcactggtct tcgcggcgaa
    10261 acgtgatggg tcccaaaaca tacatcctgc taacagaagt gccatcacga aggaaagcgc
    10321 ccgcaggggg cgcttgtcca ttaccgcata caaggttgcg ataatcttac tcatcagatg
    10381 tcttcttgac cggctttgcc tgattcgacg tgctcaagcg ccagcgcggt gattacccca
    10441 aacgaacagg caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta
    10501 cttagtacag agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt
    10561 tccagtaaca ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg
    10621 tcatgacgtt aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca
    10681 tcatggtgct ggacggcatc acaaacggga acattgcgat accggcagtc aggatgatgc
    10741 aggccagcgt cagggaggag aaaacaaacg cccacgcggc tttatccata cgtgcagtca
    10801 ggatggtcag cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt
    10861 tgttgaagtt aaccagccat gcgccagctt cacgaaccac ttctttattc agcgggttag
    10921 aggctgcgta atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc
    10981 atacgccagc cagtgcgaag cagaccagcg tcaccagtgc cgccacctga gccgttgcac
    11041 gggtacgcag gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa
    11101 tgatcatccc tacgctcacc acacctgcca gcaggccgaa cgggttaagc agctggaaga
    11161 agttaccggt gtagtacaga cgcagatatt catcaacgtt gaatggtacg ccctgcaaca
    11221 ggttaccgaa cgctacacca atcaccagcg gcggaacgaa gctaccaatg aagatgcccc
    11281 agtcccacat gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg
    11341 gacggaagaa caaagacgcc agtacgagga tcatcgccac atagaagccg gagaacgcag
    11401 cggcatagac catcggccag gcagcaaaca gtgcaccgcc tgcggtgatc agccaaacct
    11461 ggttaccgtc ccagtgtggt gcgatggagt taatcataat tcgacgctcg gtgtcgttac
    11521 gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac
    11581 caatcagcag aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca
    11641 tttgacgact cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat
    11701 agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt
    11761 ctgccaccag gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc
    11821 ctgcggtcag tgacgagttc gccacagctg tcggcagcac ttcaccgata gcccacggtt
    11881 ggcggccata ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac
    11941 cgtacagcgc ggcgcgcagc agccattttt tctcgccaat gcggttgcgg atgacactcc
    12001 agaaagagag cgcaatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg
    12061 cgaagtacag cggcgctaca cgcggaatgg agtctttggt tgcctgttga atctgagctt
    12121 cagtcgcatc agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgagatctt
    12181 tcttcatgct attgaactgg tcacgaacgg cctggtcagt agaaccagaa cgcagttgtt
    12241 cgagcagaga gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagtt
    12301 ctttcagacc gataactgga gtatccacgg aacgcgttgc aatgatgccc agcgcgtaag
    12361 ggatctggat cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacagagtaa
    12421 aggcagcagg tgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct
    12481 gcacgtcgcc catttcgtag ccggattcat cacccagaac aataacagac agaacggcag
    12541 ccataccgaa gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt
    12601 tcagcatata ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagaag
    12661 ctacggtgtg aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca
    12721 ccatctccat acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc
    12781 cgtttgcaac cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga
    12841 cacacatatg ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg
    12901 tggattcgag gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc
    12961 ctacatagtg ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg
    13021 tagccacacc cagagcaaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt
    13081 tataaatctg tttgccggaa aggacgtaga ccgtttccat aatggccagc aggaacgcca
    13141 taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc
    13201 gcgacagttc gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga
    13261 gtgaaccccg ttagaaaggg tcacacgcat gccccaatac aaaattattt gctcgcctct
    13321 atcgttgcca tttcatcaaa agaagaaaag acaatgataa aaaagttaca aatatgttaa
    13381 tgaataaacc aaatttatcc cgtaattata ttacgctgca aaatccttac aataaacagg
    13441 tttttattga gcaaatttgc atttttcgac agtgatcaat ttatagcgaa attgtcacga
    13501 tcccaccaat ttgatctaca acaatttatc gtgttttgca gatttttttc aagaattaaa
    13561 aattgattta tatcaaaatc aatcagatat gttaaccgct cgtaaatcat gaatcattga
    13621 tgggtttgtt gttaataaat tgtagcaaaa taacacagta aaactaccca catggaaata
    13681 tatagataaa aaaccagtat tttatttaac agaaccgcct tatcgacttt ttataatact
    13741 agttagttag agtagctact ttgttgccat tttacataac atatttttat caacgttaat
    13801 tgat
//
LOCUS       DAJOTB010000123        13775 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJOTB010000123 DAJOTB010000000
VERSION     DAJOTB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.123"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(505..930)
                     /gene="arsC"
                     /locus_tag="OHN28_003639"
     CDS             complement(505..930)
                     /gene="arsC"
                     /locus_tag="OHN28_003639"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCQ2298957.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1265..1348
                     /locus_tag="OHN28_003640"
     CDS             1265..1348
                     /locus_tag="OHN28_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCQ2298958.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(1402..2754)
                     /gene="gorA"
                     /locus_tag="OHN28_003641"
     CDS             complement(1402..2754)
                     /gene="gorA"
                     /locus_tag="OHN28_003641"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCQ2298959.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2826..3668)
                     /gene="rlmJ"
                     /locus_tag="OHN28_003642"
     CDS             complement(2826..3668)
                     /gene="rlmJ"
                     /locus_tag="OHN28_003642"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCQ2298960.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3871..5913
                     /gene="prlC"
                     /locus_tag="OHN28_003643"
     CDS             3871..5913
                     /gene="prlC"
                     /locus_tag="OHN28_003643"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCQ2298961.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5921..6673
                     /gene="rsmJ"
                     /locus_tag="OHN28_003644"
     CDS             5921..6673
                     /gene="rsmJ"
                     /locus_tag="OHN28_003644"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCQ2298962.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPAQLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6722..8191)
                     /gene="dtpB"
                     /locus_tag="OHN28_003645"
     CDS             complement(6722..8191)
                     /gene="dtpB"
                     /locus_tag="OHN28_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCQ2298963.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     QLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8508..8942)
                     /gene="uspA"
                     /locus_tag="OHN28_003646"
     CDS             complement(8508..8942)
                     /gene="uspA"
                     /locus_tag="OHN28_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCQ2298964.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9333..9668
                     /gene="uspB"
                     /locus_tag="OHN28_003647"
     CDS             9333..9668
                     /gene="uspB"
                     /locus_tag="OHN28_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCQ2298965.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9739..11238)
                     /gene="pitA"
                     /locus_tag="OHN28_003648"
     CDS             complement(9739..11238)
                     /gene="pitA"
                     /locus_tag="OHN28_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCQ2298966.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11470..12663
                     /locus_tag="OHN28_003649"
     CDS             11470..12663
                     /locus_tag="OHN28_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCQ2298967.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12750..>13775)
                     /locus_tag="OHN28_003650"
     CDS             complement(12750..>13775)
                     /locus_tag="OHN28_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCQ2298968.1"
                     /translation="KLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLAL
                     FTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVI
                     FGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLF
                     GFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWC
                     YWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTC
                     SLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSIL
                     EAGSAKK"
BASE COUNT         3536 a         3459 c         3602 g         3178 t
ORIGIN      
        1 ataattacct ttgacttctc ttttatttcc atctggtaat atttgagtgc aaaaaaaggc
       61 aataataata tcgcaaaaga aaacgttaac aactatcgaa aatgctcgtt ttaaaaaggt
      121 atgatgttct ctgatagtgt gtgaagtgat atttttgtga tgttttagat atgtaataat
      181 aaaatgggtt aacgtatgtt gaaattattt attgtgcaaa ccactgatag atcataatcc
      241 agtacatctt tacagtgctg cactgtatcg ctgataacta agcagctcac tgatacacca
      301 tgctggacgc gataattgga atctcacgca gggagtactg tgtaaatagc aaaatcgcct
      361 gatgcgctac gactgtcagg cctacaaata acatacaatt catcgaatta tcataacgtt
      421 gtaggtcgga taagacgcgt cagcgtcgca tccgacataa ataaagcgca tttttctaac
      481 aacctgtggg ggaatatagc cgctttattt caggcgatta cccgcctcat caaccacttt
      541 ctcgccatct tccttggtga acgcgccttt ttgcgcatct ggcagaattt ccagcacgac
      601 ttctgaaggg cggcacaggc gagttccctg tggcgtcacc acaatcgggc gattaatcag
      661 aatcgggtgc tgaagcataa agtcgattaa ctgatcgtga gtaaatttat cttctgcaag
      721 gcccagttcc tcatacggct cgacgttttt acgcagcaac gcgcgtacgg taatccccat
      781 atcggcaatg agttttacca gctcatcgcg cgtcggtggt gtttccagat aatagataat
      841 ggtcggttct gtaccgctgt tgcggatcat ctccagcgca ttacgcgacg taccgcaggc
      901 cgggttgtga taaatggtaa tgttgctcat atcagtatct cattacaatg tgaaagagag
      961 gtgtagcgcc agcgccacca gcgttacaaa ccaaagcgga caagtcaaaa acacatatgg
     1021 tttaacgaat atgtaagtgt agagaaaggt gtaggtgtga attaagcgtt gggcattcat
     1081 ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt cgcgtcggtt tttttactgt
     1141 tcagccctga gttggtggct ctggctggag tgagatagcc cacatctaac aatgagacat
     1201 gctccgtgac aggcaatgga tgagtaagcg gatgcattct cactccatcg catggagaaa
     1261 acgggtgatt gataaagcaa tcatcgttct tggggcgtta attgcgcagc tggaactgat
     1321 ccgctttctg cttcagcttc tgaactgata gcggaaacgt agctaagggc taagagcaca
     1381 ctactcttag ccctttaaca tttaacgcat tgtcacgaac tcttctgctg ctgtcgggtg
     1441 aatggcaacc gtgttgtcga agtctttttt ggttgcaccc atcttcagcg ccaccgcgaa
     1501 gccctgcaac atttcgtcca taccaaaacc aatgccgtga ataccgacaa tcttctcttc
     1561 cggtccaatg cataccagtt tcatgcgaca cggctggcgg tgagtggtga cggcggtata
     1621 catcgcggtg aaagaggatt tatacacttt cacctgatcg tcgccgtact gctcgcgcgc
     1681 ctgcggctcc gttaaaccaa cagtaccaat cggcggatgg ctgaagacca cggtcggaat
     1741 gttgctgtaa tccagatgct catccggctt gttattaaac aggcgttcag agagacggcg
     1801 acctgctgca actgccaccg gagtcagttc cactgcaccc gtgttatcgc ccaccgcgta
     1861 aataccttca acgttggtgt tttgatattt atcgacgacg atatagcctt tttcgttggt
     1921 tttaacgcca gcggcttcca ggttgatgtt gtcattggca ggctcgcgac caatcgccca
     1981 aatcaggcaa tccaccgttt cactgcgacc atcttccagc tccagcgtca ggctgccgtc
     2041 ggcatttttc accaccgctt tcgggatggc gttggtatgc aactgtgggc cttcggcgtt
     2101 catcacttcg accagcgttt cagagatcat cgggtcgaag ctgcgcagcg gcgcatgttt
     2161 acgcacaaac agatgcgtct tcgcaccgag gccgttaatc acgcccgcca gttcaacggc
     2221 gatgtaaccc gcgccaacaa ccgctacgcg ctctggcaaa gccggaaggg cgaagaagcc
     2281 atcagaatca ataccgtatt ccacgcccgg aatatccggg tggctcggac gaccaccggt
     2341 ggcgatcaga atatgatcgg ccgtgatggt ttcgccgttg acttcaatgg ttttggcatc
     2401 aacgaagcgg gcaaagcctt tgattacatc aacgttattt ttaccgagca cgttctcata
     2461 ggaagtatga atacggtcga tataggcggt acggctggcg atcaatgttt cccagttgaa
     2521 tttattgata gtggtatcaa agccgtaatc cgggccgtac atatggatcg cttcacggat
     2581 ttgtgccgcg tgccacatca cttttttcgg cacacagcca acatttacgc aggtgccgcc
     2641 cagctctttg gcttcaatca gtgcgcattt ctggccgtac atagccgcgc ggttgatgga
     2701 ggcgataccg ccgctgccgc cgccgatggc gatgtaatca tagtgtttag tcatgacata
     2761 ttgtccttat cgttgattac cgcgattgta gcgcgagacg taataggtgc cagcaatggc
     2821 tgcaattact ctggcacgat ccagcttacg gtggcgtgcc cggtgcctgc tggaaccagt
     2881 ttgctgtgca gccacggcag cacgttattc atctgctgtt ccagcttcca cggcgggtta
     2941 atcacaatca tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag caccgccagt
     3001 tcaatttgca gaattttgcg aatgccggtc gcttccaggt cgtggatcat gcgcttaatc
     3061 tgctggcgca gcaccactgg ataccacaat gcgtaagtgc cagtggcgaa acgtttgtaa
     3121 ccttctgcta tcccgctgac caccgcctga tagtcggttt tcatttcata cggcggatcg
     3181 ataaggatta aaccacggcg ggaaaccggc ggtagtttgg ccttaagctg ctggaaaccg
     3241 tcggcttttt caacgcgcgc acgattgtct ttctggaatt ccgaacgtaa caacggataa
     3301 tcgctcgggt gcagttcagt cagttgcagg ctgtcctgtt cacgcagtag ctggcgcgca
     3361 atcaacggcg aaccggggta ataacgcaac tgcccgctac ggttgaagtg ttttaccaca
     3421 ttgatgtacg cctccagctc cgcaggcaaa tcgtcctgct gccagatacg ggcgatacct
     3481 tcgagatatt cgccggtacg ctcggcatgt tcgctgccta actgataacg cccggcccct
     3541 gcgtgggtgt cgagatagag aaacggttta tctttctctt tcagcgactc gatgatcagg
     3601 ctctgaacgg tatgtttaag gacgtcggcg tggttgccag cgtgaaagct gtggcgataa
     3661 ctgagcatgg gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa
     3721 agtctatcag gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat
     3781 tgaaattcac tacacttaac cccatgctac aaacattatg taaagcgcct gttgagcgct
     3841 tccttaacct ctttaaccag gactgcgcta atgacgaatc cgttactgac tccctttgaa
     3901 ttgcctccgt tttctaaaat tctcccggaa catgtcgttc cagccgtgac taaggcgctg
     3961 aacgactgcc gcgaaaatgt ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa
     4021 aatctctgcc agccgttggc ggaagtggac gatgtgctgg ggcgtatctt ctccccggtc
     4081 agccatctga actcggtgaa aaatagcccg gaactgcgtg aagcgtacga acaaaccctg
     4141 ccgctgctgt cagaatacag cacctgggta ggacaacatg aagggctgta taaagcatat
     4201 cgcgacctgc gcgatggcga tcattacgcc acgctgaaca cggcgcagaa aaaagcggtt
     4261 gataacgcac tgcgcgactt cgaactctct ggcatcggtc tgccgaaaga gaaacagcag
     4321 cgttacggcg aaattgccac tcgtctttct gaactgggca atcagtacag caacaacgtc
     4381 ctcgatgcga cgatgggctg gaccaaactc gttaccgacg aagcggagct ggcggggatg
     4441 ccagaaagcg cgctggctgc ggcaaaagcc caggccgaag cgaaagagct ggaaggttat
     4501 ttgctgacgc tggatatccc aagctacctg ccggtaatga cctactgcga caaccaggcc
     4561 ctgcgtgaag agatgtatcg tgcttatagc actcgtgctt ccgatcaagg tccgaacgcc
     4621 ggtaagtggg ataacagcaa ggtgatggaa gagatcctcg cgctgcgtca cgaactggcg
     4681 caactgctgg gctttgaaaa ctacgccttc aaatcacttg ccactaaaat ggcagaaaac
     4741 ccgcagcagg tgctggattt cttaaccgat ctggcaaaac gcgcgcgccc gcaaggcgaa
     4801 aaagagctgg cacaactgcg cgccttcgcc aaagccgaat ttggcgtcga tgagttgcaa
     4861 ccgtgggata tcgcttacta cagcgaaaaa cagaaacagc acctctacag catcagcgat
     4921 gaacaactgc gtccgtactt cccggaaaac aaagcggtta acggcttgtt tgaagtggtg
     4981 aaacgtattt acggcatcac cgctaaagag cgtaaagatg ttgatgtctg gcatccggat
     5041 gtacgtttct tcgaactata tgacgagaac aacgaactgc gcggtagctt ctacctcgac
     5101 ctgtatgccc gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt aggccagatg
     5161 cgtaaagccg acggttcgct gcaaaaaccg gtcgcgtatc tgacctgcaa cttcaaccgc
     5221 ccggtaaatg gtaaaccggc gctgtttacc catgacgaag tgatcaccct gttccacgag
     5281 ttcggtcacg gcctgcacca tatgctgacc cgcattgaaa ccgctggtgt ttccgggatc
     5341 agtggggtgc cgtgggatgc ggtcgaactg ccgagccagt ttatggaaaa ctggtgctgg
     5401 gagccggagg cgctggcgtt tatctccggt cactatgaaa ccggcgagcc gctgccgaaa
     5461 gagttgctgg ataaaatgtt ggcagcgaaa aattaccagg cggcgctgtt tattctgcgc
     5521 cagctggagt tcggcctgtt cgatttccgc ctccatgccg agttccgtcc ggatcagggg
     5581 gcgaaaatcc tcgaaactct ggcagaaatc aagaaactgg ttgccgtggt accgtctccg
     5641 tcatggggcc gtttcccgca tgctttcagc cacatttttg ccggtggtta tgcggcaggt
     5701 tactacagct acctgtgggc cgatgtactg gcggcagatg ccttctcgcg ttttgaggaa
     5761 gagggcattt tcaaccgtga aactgggcag tcgttcctcg acaacattct gagccgtggc
     5821 ggttcagaag agccgatgga gctgttcaaa cgcttccgtg gtcgtgaacc acagctggat
     5881 gcgatgctgg agcattacgg cattaagggc tgatcattca gtgaaaatct gcttaattga
     5941 tgaaacaggc gccggagacg gcgccttatc tgttctggcg gcccgctggg ggctggaaca
     6001 cgatgaagac aacctgatgg cgctggtgtt aacgccggag catctggaac tgcgcaagcg
     6061 tgatgagccg aaacttggcg gcatctttgt tgattttgtt ggcggagcga tggcgcaccg
     6121 acgcaaattc ggcggtggtc gcggagaggc ggtggcgaaa gcggtgggca ttaaaggcga
     6181 ttatttgccg gatgtggtgg acgccaccgc agggctgggg cgcgatgcct ttgtgctggc
     6241 ttcggtcggc tgccgcgtgc ggatgctgga gcgtaatcca gtggttgccg cgttgctcga
     6301 cgatggtctg gcgcgtggtt atgcggatgc ggaaatcggc ggctggttac aggaacgttt
     6361 gcagttgatt cacgcctcca gtctgacggc gctgagcgat atcaccccgc gtccgcaggt
     6421 agtttatctt gacccaatgt tcccgcataa gcagaaaagc gcgctggtga aaaaagagat
     6481 gcgcgtgttt cagtcgctgg tggggccgga tcttgatgcc gatggattgc tggagcctgc
     6541 gcagttgctg gcaaccaaac gcgtggtggt aaagcgtccg gactatgcgc cgccgctggc
     6601 gaatgtcgcc acgccaaacg cggtagtcac taaagggcat cgctttgata tttatgccgg
     6661 aacgccggtg taaaagtaaa gcggggtagc gataacgcta ccccgctttc tgcaagaata
     6721 attaatggct ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc aatagcatca
     6781 ctactgcaac gcccagcgta accaggccaa tcttaccaaa cacgttggta tagacgggca
     6841 acgtctcaag tggatcggta atgttgtctg gtactgcggt aaatgttgcc acatagccgc
     6901 ccagcaagaa cgcggcagcc tgcgtcagga accacatccc gagaataaag cccatcaaat
     6961 gctgcggcac cagggcagca atcatcgcca ggccaagggc gctaataaac agttcaccta
     7021 agctctggaa taagtacacc agcacgataa accatggcga tgtcagccct tgggcatccg
     7081 caaaccacat tccagccgct gcagccgtca aaaagcccag tgagcacata aacatgccga
     7141 gagtaaactt catcggcatc gagagatctt tgcctttgtt acccagttgc gtgtaaatgc
     7201 ctgccagtat tgggctggcg agtaccaccc agaacgggtt aagcgcctgg aagctgaccg
     7261 ggttgatgga aaagccgaga atttcatgat gcacgttgtt gatggcaaag aagttcagcg
     7321 acgttggcat ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg
     7381 cgacaaacat tttattgcgc ccggttttat ccagcttgaa tgcctgacga aagaagatga
     7441 tggtgacgac gatggagaga acaatcagca ccagattggc gacttctacg ttgtgcatca
     7501 gccaggcgca gacgaagatc atcaccacgc tgccaagtaa tacgtacaac agtttactga
     7561 agctcatcgg ccggaagtcg ggttcagaac caatatcttt caccattcca cgacaggcga
     7621 tgtaaaccag taatgcgata attaaccctg caccgcacag gttgtaggtg actgaatagc
     7681 cgaatttatc agcgatcaca ggggccagcg ataacgctat caacgagccg atattgatcg
     7741 acatatagaa cagggtgaat gcgccatcaa gccgtggatc tttcggcgga tagcatttcg
     7801 aaagcaagct ggctgggtta gctttaaaca ggccgttacc gacagcgata gtccccaggg
     7861 cgatgaaaat caggtcaggc ttaagtagcg acatgccggt catgaagtag ccaatcgcca
     7921 gcacaagtgc tccgagaaca atggtgcgtt tggttcccag caggtggtcg ccaacatagc
     7981 caccaatgga aatgaggcca tagaccagcg cagcaaaagc accaaaagtg acaaaagcct
     8041 gctcttgcga gaacccaagc tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt
     8101 agtagccgaa tcgctcccat aactcgacaa aaaagatcat gaaaaatggg cgaggttgct
     8161 gcagcatccc catgggtgtt gttgtattca tatttattaa ccttccaata ccatcctgaa
     8221 aagtatgaac gcgtaaggga taacgcgcga aaaagtttac atctgtcaga gattcttaat
     8281 attgatgcgg cgaatagatt aatacactat tacaacagaa aataaccagc tgatcacact
     8341 aatgatagaa aatatgatgg tgttaaatgt tgtattggca ggggttttaa ttgcgtacgc
     8401 agaccgcagg ccagataagg cgtttacgct gcatcaggta acaaggcgcg ccaaaaagca
     8461 aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga gggaagatta ttcttcttcg
     8521 tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg acgtgcggaa
     8581 gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc catatcgtat
     8641 ttcttgattg catcgaccag tacctggccc agatcgccgc tgccgctcag ggtttcagtg
     8701 attgggtagc ctgcattggt tgaaagctcg gtcagcgcat gatgtgtctc ttcagagatg
     8761 cgtttctgca tatcacccag attcacatcg ataagcccgg tgtataggtc agagtagttt
     8821 acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga gactgctttc
     8881 tctaccagaa ctttgctttc cggggagagg tcgaccgcga tgagaatgtg tttataagcc
     8941 atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt cttgtgccgt
     9001 tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa cccagccgta aagattcagt
     9061 gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc ttgcttactc
     9121 gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc tggaaaaaaa
     9181 acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc acaaaagcgg
     9241 tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag ccccggggtg
     9301 gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca ttattttggg
     9361 ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta cgcgcgttgt
     9421 tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga gggggctttt
     9481 ttacctcaca tggccaacca aacaaacagg tgcgtctcgt ttggtatatc tatgcccaac
     9541 gttatcgcga tcatcacgat gatgagttta ttcgccgctg tgagcgggtg cgtcggcagt
     9601 ttattctgac cagcgcattg tgtggtctgg tggtggtcag cctgattgca ttgatgattt
     9661 ggcattaagc acttcagcaa taatgacgca taaaaaaagc gggccagtaa actgacccgc
     9721 tcgttttggt gcgtacaatt acaggaactg caaggagagc cagtacagcc cgccggaaag
     9781 cagtaccgca gccggaaggg taaacaccca ggccatcaga atactggtta cggttttacg
     9841 ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca gaagaggaga gtacgtgagt
     9901 agtggaaacc ggcatcccgg tataacttgc caggccgata gacacagccg ccgtcatctg
     9961 ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg attttctcac cgatagtcgt
    10021 tgccacacga cgccagccaa tcatcgtacc gatacctaac gccagcgcga ccgccatgat
    10081 gatccacacc ggcgcatact cgatggtgct aagcatgtcg gacttcagtt tcttcaacag
    10141 gcgctgatcg tcagcactca cgccaggcat tttcaccact ttatcgatag tgtcagaaac
    10201 gcacagcata atgcggcgca tctggctacg ttgatcaagc gacagcttgt cgtagctttc
    10261 cacatcggta gtcaacatgc ctttcaggcg gttgagcgcg ttaatggtat tcgacggatg
    10321 gcaatggaac tccgcgggtt gcgttgcgcc agcttccgga gccggtacta actgatcagc
    10381 accggtcgct tgtttcagca gtgcaggatg ctgctcaaag taagcttcga cgttgttgat
    10441 ggcatcacgg gtacgggtga tttcgtagcc agtggcattc atgttcacca cgaagcctgc
    10501 tggtgcgacg ccaatcaata ccaacataac cagaccaatg cctttctgac catcgttcgc
    10561 gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc gcaatacgcg tccagaacgg
    10621 cggctttttc ttgccgtctt tcttttcacg ctccgctggg gtcaggtgga tacgggcgcg
    10681 tttcttggtg ccgctccagt agcgacgcag caagaaaatc agaccgccag caaacaccag
    10741 gccgacaata ggggaaacga tcagagaacc gaaaatactt aatactttcg ggatattgag
    10801 tgcatccacc actgacgtcc cggtcatcaa cgcattggtt aaaccaatcc cgatgatcgc
    10861 gccaatcagc gtatgagagc tggatgcagg taaaccaaag taccaggtac ccaggttcca
    10921 gataatcgcc gccagcaaca tagagaacac catggcaagg ccatgagacg atcccatatt
    10981 aagcagcaga tccgtcggca gcatatgcac aatggcatag gcaacactca gaccacccag
    11041 caaaacaccc aaaaagttga ataccgccgc cataaccacg gcgagctgag aacgcatcgc
    11101 gcgggtatag ataacggttg ccacggcgtt ggctgtgtca tggaaaccat tgatggcttc
    11161 gtagaacagc acaaaagcca gtgcaagcaa taataacagc ccggtatgca aatccaggcc
    11221 agcaaacaaa tgtagcatag gacgttacgc cattttgagg acatgaacgc ggcgcattat
    11281 cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt tttttgattt gcctcctgta
    11341 tggattccac tcaaaaaata attatcttat ataattcagg caaatacttc cttttagtaa
    11401 tattgatgct ggtgcgacca ctgaggaatc tttacaattc acgcccgttt tttcttagag
    11461 gagcgcaacg tggaaaggtt tgatgccatt attataggcg ctggtgcggc aggtatgttc
    11521 tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc tgatcgataa tggtaaaaaa
    11581 ccagggcgca aaatcctcat gtctggcggt gggcgctgca actttaccaa cctttatgtc
    11641 gaaccaggcg cttatctgag ccagaatccg catttttgta agtctgcact cgcgcgtttt
    11701 acccagtggg atttcattga tctggtcaat aaacacggca tcgcctggca cgagaaaacg
    11761 ttagggcagc tcttctgcga tgactccgcg cagcagattg tcgacatgct ggtggatgag
    11821 tgcgagaagg gcaatgtggc cttcagattg cgtagcgaag tgctgagcgt ggcgaaggat
    11881 gatacaggct tcacgcttga actgaacggc atgactgtcg gttgcgaaaa gctggtcatt
    11941 gcgaccggtg gactgtcaat gccggggctg ggcgcatcgc cgtttggtta taagattgcc
    12001 gaacaattcg gcctcaacgt gctgccgacc cgtgcgggtc tggtgccatt cactctgcat
    12061 aaaccgttgc ttgaagagtt acaggtgctg gcgggcgtgg ctgtgccttc cgtgattacc
    12121 gctgaaaacg gtaccgtttt ccgtgagaac ttactcttca cccaccgcgg cttatctgga
    12181 ccggcggtgt tgcagatttc cagctactgg caaccgggtg aatttgtcag catcaatctg
    12241 ctaccggatg tggacctcga aaccttcctg aatgagcagc gtaatgcaca tccgaatcaa
    12301 agcctgaaaa acacgctggc ggttcatctg ccgaagcggt tggttgaacg gttacaacaa
    12361 ctcgggcaaa tcccggatgt ctcgctaaaa cagctcaacg tgcgtgacca acagacactg
    12421 attagcacat tgactgactg gcgcgtacaa cccaacggca ctgaaggcta tcgcactgcc
    12481 gaagtgacgc tcggcggcgt ggacaccaac gaactctctt cacggacgat ggaagcgcgc
    12541 aaagtgcctg ggctgtactt catcggcgaa gtgatggacg tcaccggctg gctggggggc
    12601 tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg cgcaggattt ggtggcagaa
    12661 taagaagcaa aatgcgctct cgataccaga gggattaacc tgaataaaac cagataaggc
    12721 gctcaacgcc ttatctgaag ccttttttat tattttttcg ctgaacccgc ttctaatata
    12781 gaaacgatgg aaaacagtgt aaaacaaact gcacctaagc caaccagaac tcgcgaagga
    12841 ataaagtaag ccatatcggt ttgcgcaatt tcagccagga acgcagcaag gaacagacaa
    12901 aacagacagg taaataccgg aatcatgggt attctgttgg ctaacgaaca ggtgcgtcgc
    12961 cataccagcg ctaacaacca cactttggag aaaatactgt agcagatcat ccccaggcaa
    13021 atgagaataa tgccgggggc caggcgggcg cttgcatctg aactgattaa tacgtagata
    13081 ccaaaaataa tggtaagtga gccaagcaaa ataacccaat agcaccacag ccaatgttct
    13141 ttcactgaga acgtattccg cgtttgatgg acaatagtgg cgacaaggcc aataagacaa
    13201 gcgcaaattg ccgttaaccc cattagtacg tgacctgcaa cataatgtgg tgtgatatct
    13261 gcactacgta acagtatgac tgaccaaatg aaaccaaaga gagtaagcag aacgggaacg
    13321 gcaattaaac aattaccgat tgttgaagaa taggcctgca atggcgtacc gtcactctta
    13381 ctgcccgagg cgtttttggg gatcaacaga aaatgtccgg aagatgccgc tacggttgat
    13441 acacaagcgg caatcatgcc aacgccgaaa ataacatgac cggcgacaaa ttcatctgcc
    13501 atcacattat tgcttgccaa cagtgaccat ccccaaatca tagttgtagc agaaccggca
    13561 taaccaataa caggaaataa cctgttgtag aatttattca tgccgtgagt taactgagat
    13621 ataataataa atgcggtagt gaacaatgcc agacatatcg cagaaagaga aatcagaaca
    13681 tgccctgcaa cgaaatattc actttgccca gaaccggtta atacaaactc acctaatgcg
    13741 atgcatatta tacccatcag taaaggaatt aattt
//
LOCUS       DAJOTB010000124        13550 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJOTB010000124 DAJOTB010000000
VERSION     DAJOTB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.124"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            11..1003
                     /locus_tag="OHN28_003651"
     CDS             11..1003
                     /locus_tag="OHN28_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCQ2298969.1"
                     /translation="MLASTLFLHHLPSFAQSVKFWASLTLPEAQHITRIVSAGAPADL
                     LLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSLEGLMALHPDL
                     VVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLGEEHRAAEQAN
                     LASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLGLHNVAQIADR
                     HGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRILFLSGLPFGWL
                     DAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADYQKLVAS"
     gene            1003..1983
                     /locus_tag="OHN28_003652"
     CDS             1003..1983
                     /locus_tag="OHN28_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2298970.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            1980..2738
                     /locus_tag="OHN28_003653"
     CDS             1980..2738
                     /locus_tag="OHN28_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2298971.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2748..3560
                     /locus_tag="OHN28_003654"
     CDS             2748..3560
                     /locus_tag="OHN28_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCQ2298972.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3557..4411
                     /locus_tag="OHN28_003655"
     CDS             3557..4411
                     /locus_tag="OHN28_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCQ2298973.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4437..6407
                     /locus_tag="OHN28_003656"
     CDS             4437..6407
                     /locus_tag="OHN28_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCQ2298974.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6457..6711)
                     /locus_tag="OHN28_003657"
     CDS             complement(6457..6711)
                     /locus_tag="OHN28_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2298975.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6912..7646
                     /locus_tag="OHN28_003658"
     CDS             6912..7646
                     /locus_tag="OHN28_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCQ2298976.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7648..8259)
                     /gene="emtA"
                     /locus_tag="OHN28_003659"
     CDS             complement(7648..8259)
                     /gene="emtA"
                     /locus_tag="OHN28_003659"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCQ2298977.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8359..9273
                     /gene="ldcA"
                     /locus_tag="OHN28_003660"
     CDS             8359..9273
                     /gene="ldcA"
                     /locus_tag="OHN28_003660"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCQ2298978.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            9368..11104
                     /locus_tag="OHN28_003661"
     CDS             9368..11104
                     /locus_tag="OHN28_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCQ2298979.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11161..12231)
                     /gene="dadX"
                     /locus_tag="OHN28_003662"
     CDS             complement(11161..12231)
                     /gene="dadX"
                     /locus_tag="OHN28_003662"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCQ2298980.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12241..13539)
                     /gene="dadA"
                     /locus_tag="OHN28_003663"
     CDS             complement(12241..13539)
                     /gene="dadA"
                     /locus_tag="OHN28_003663"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCQ2298981.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEALSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         3119 a         3669 c         3507 g         3255 t
ORIGIN      
        1 gtttacacag attcttgcgt cgacattgtt cctgcatcat ctgccgtcct ttgcacaatc
       61 agtcaaattc tgggcctcac tgacgcttcc cgaagcccaa cacattacac gcatcgtcag
      121 cgcaggcgcg cccgccgatt tactattgct ggctgtcgcg ccagaaaaaa tggtcggttt
      181 ttcctctttt gattttgccc gtcaggcatt aattccattg ccagagcaca ttcgccagtt
      241 ccccaggctg ggacgactcg ccgggcgcgc cagcacactc tcgctggaag ggcttatggc
      301 gttacatccc gatttggtcg ttgattgcgg caatacggat gaaacctgga tctcccaggc
      361 acggcaggtt agcgaacaga cacaaatacc ctggttattg cttaacggga aactggaaca
      421 atcagcagaa cagttaacaa cgcttggcaa aacgttaggc gaagagcacc gcgccgcaga
      481 acaagccaat ctcgccagcc gcttcgttgg tgaagctcag gcattcgcca cctcacccgc
      541 cgctaatctc agcttttatg ctgcgcgcgg tcctcgaggg ctggaaacgg gcttacaggg
      601 ttcgttgcat accgaggcgg cggaattatt aggtttgcac aacgtcgcgc aaatagccga
      661 tcgccacggt ctgacacagg tttccatgga aaatctcctg cgctggcagc cggatattat
      721 tctggttcag gaggccgtta ctgcagattt tattcgtcgt gatcctctct ggcagggcgt
      781 gaaagcggtt gcggaacaac gcatcttatt tttaagtggc ctgccctttg gctggctgga
      841 tgccccgccg ggaatcaacc gtcttctggg attacgcaga cttcacgcct ggctggatcc
      901 cgccatcaat cgccagttta aaagtgacat gcagcattac gcccaactgt tctggcattg
      961 ttcactcagt gacgccgact atcaaaaatt ggtggcgagc taatgagaac cgtaaacgga
     1021 tgtattttgc tgctggcagc tatcagtatc acttttgccg ccgtaagtgg ggcttatcat
     1081 ctcgatatgc agcaactgct ggcgctcatt cttcgtcaag aaaatgttcc tgtgcaggaa
     1141 caaattgtct tctggcaaat ccgcgtgccg cgtattcttg ctgcgctgtt tctgggcgcg
     1201 gcgctggcag gggccggaac cacctatcag ggaatgttcc gtaatccctt agtgtcaccg
     1261 gatattttag gcgtctccgc cggtgccgga cttggtgcct gcgcggcaat cctgtgggga
     1321 ttatccattg tttacatcca gctgtacgca ttttgcggcg ggctgatggt ggtcgcgggc
     1381 gtctggttga ttacgcgtcg ggtgacccgc cacgatccga tcttaaccct ggtgctggta
     1441 ggcattgcgt taggaacgtt gtgcggagcg gggatatcgc taataaaaac gctggccgat
     1501 ccttacaccc agttaccgtc aattaccttc tggttgctgg gggggctgtc caccgtgacc
     1561 ttacgcgatc tctgctatgc tgcaccgatc attctcatag gctccttgcc cctgtttttt
     1621 ctccgctggc gcatgaattt actcacgctt tccgacgacg aagcccgttc gctgggactg
     1681 aacgtgacac gtctgcgctt tggattgatt gtctgcgcca cactcatcac cgccagcacg
     1741 gtagcaatag caggcataat tggttgggtc ggcctggtcg ttccgcacat tgcccgcttg
     1801 ttgaccggtc ataaccatca gcagctgttg cccatggcaa tgtgtactgg cgcgattctg
     1861 ctcctgctta ccgatacgct ggcacgcagc attggaacaa cagaaatccc acttggcatt
     1921 ctgaccgctt ttgttggtgc accgtttttc ctgtttttgt tgttacgcgg aggtcgccag
     1981 tgactttgct tgccgtccgt catgcgtctc tgggttactc ccgccatccg gtgctacgcg
     2041 acgtgtcctt caccctgtcg caaggaacaa tttgttgtct gttaggagcc aatggttgcg
     2101 gcaaaaccac actcatgcgc tccattcttg gcgttatacc gttattaaaa ggcgagatct
     2161 tactggatag cctccctgtg cagacactca gccatcgcca gcgcgcgcag gctattgcct
     2221 gggtgccgca ggcgcacgac ggcatttttg cttttagtgt gctggatatg gtgttgatgg
     2281 gcctggcccc caccataggc gctttttccg ttcccggtaa gcaagaacgc ctgaaagcaa
     2341 cggagcaact ggaaaagctg ggcattttgc atctggcgac acgacgctgg aatacgctta
     2401 gcggcggtga acgtcagctg gctttaatcg cccgtgcact cgtccagcaa ccgcgcttgt
     2461 tattactgga tgagcctgcc tccagcctcg atttcggtca tcagatccaa cttctggaca
     2521 cgctggcgca gctcaaaaat aacggcatga caatgttaat gtcgactcat catccgctcc
     2581 atgcaaacgc cattgccgac agcattatcc aggtagagcc agatggacga gtaacacaag
     2641 gcttaccgac cgaacagtta acaacgaaaa aactggctgc cctgtaccgg gtttctgccg
     2701 accagattca ccatcacctc tctgcaatta agcactaagg aaaaatcatg ctcattgacg
     2761 atattgattt tgcagacctt taccttcagc aactcaggct ggcccatcgc acagaaaaaa
     2821 cgccggatca ctgggatcaa cgggcggaaa aaatggcgga aaattgcgcc tcgcctacgg
     2881 acagctacct gcaacaactg acctcaaaaa ttgacctgca aggcgcgcaa accttattcg
     2941 atatgggctg cggaccgggt accgtatcgc tggcgctggc ggacaaactc acgacgattt
     3001 atggcgttga ttacagtcag gggatgctga acgttgccgc ccgtcgtgcc gccgcgctaa
     3061 aggcagacaa tgtccactgg atccagcgcg cctgggaaga ggactggagt gacctgccac
     3121 gctgcgatat tgccgtggct tcccgctcca ctctggtggc tgatatgcgc caggcaatga
     3181 gcaaactgaa taatcaggca cggcttcgcg tctatacgac tcatctggtc agcacctcgt
     3241 tcgtctctcc agccattcag cgggcggctg gtcgggaagt gatcgaatta cccaactata
     3301 tctttgcact caacgtcctg tatcagatgg gaatttatgc tcacgttgat tttatccgcg
     3361 gacaaaattg ccaacaggat aacagcacct gggaacgttt tgagcagaac gtcagctgga
     3421 gtctgggcgc gcttaacgac gatgagcgtg agcggttata tcgctggtat caacaacaag
     3481 atgctcgtgc cctcgcccct gccagccgcg actgggcgct catctggtgg gatagcgtac
     3541 cgcaggagac actgcgatga ttttcctttc tcaggcacaa atcgatgcgt tattgctgga
     3601 agatatccag ggcggtgacc tgaccacgcg agcattaaat attggacacc agcatggcta
     3661 tatagagttt tttctccgtc agggcggctg catcagcggc atttctgtcg cgtgtaagat
     3721 gttaactacg ctgggattaa ctattgatga cgcggtcagc gacggttcac aagcgaacgc
     3781 gggtcagcgg ctaatccgtg cgcaaggtaa tgccgcagca cttcatcagg gatggaaggc
     3841 agtccagaat gtgctggagt ggagttgcgg tgtttctgat tatctcgctc aaatgctggc
     3901 gttacttcgt gaacgttacc ctgatggcaa tatcgcctgc acccgaaaag caattccggg
     3961 cacccgctta ctggcctcgc aggcaattct ggctgccgga ggactgattc atcgtgccgg
     4021 atgtgcggaa accatattat tgtttgccaa ccaccgccat tttcttcatg acaatcagga
     4081 ctggtcaggc gcaatcaatc agttacgtcg ccacgcaccc gagaagaaaa tagttgttga
     4141 agccgacacg ccgaaagagg caatcgccgc gttacgcgcg caaccagacg tacttcagct
     4201 cgacaaattt agtccgcaac aggcaactga aattgcgcaa atagcaccgt cgctggctcc
     4261 ccactgcacg ctggctctga ccggcggaat taatctgaca acactcaaaa attacctcga
     4321 ctgcggcatt cgccttttta ttacctccgc gccttattac gcgacaccag ctgacattaa
     4381 agtcagtctg caacccgcag cctctattta attctttgtt aaacggaatt tattctatga
     4441 gactcaaaaa acgttatctc tgcacagcat tatcacttgc ctttacccag caggccgtag
     4501 cggctcagga gagtgacacg ctgaccgtat ggtccagtcc ggtatcatca acgacgacca
     4561 ccgttctcga tcaacccacc atgaaggccc tggataaaca gaatgtcgct caggcattaa
     4621 gtgtcgtccc cggcgtggtg ctgcaaaagt caggtagccg caacgaagaa caggtcaaag
     4681 ttcgtggctt tgatagtcgt caggtgccgg tctatttcga cggtgtgccc atttatgttc
     4741 cctatgacgg caacctcgat ctggcgcgga ttctgaccaa caatttgggg gcagttgaag
     4801 tttccaaagg gtattcgtcg ctgcttcagg ggcctaatca gatgggcgga gccattaata
     4861 tcaccaccca gaagccaaca aaacctctgg aagcaagtct gggatatcgc cagggatgga
     4921 gccgtagcca ggacaatgcc tacgatatgc atgcttcatt tgccgccagc agcgatctgg
     4981 ggtatttgca agtcagcggt agccagctaa agcaggattt tctcggcctg ccgcatggtg
     5041 taaataatga tattgcaggc aaacacggca agatgattaa ttcatcggct gatgataaac
     5101 gcggcattgt gaagctcggt tttacaccac gcgaaaacga tgaatacaca ttgacttaca
     5161 ttaagcagga tggtgaaaaa gataacccgc catacagtgg aaatagtggt cagaaatcac
     5221 gctactggca gtggccagag tatgacaaag aaagttttta ttatcaggga acgacccaac
     5281 taaacgatcg ttttaccctg aaaagtcggc tgtatcgcga cacctttgaa aatacgctaa
     5341 tgatgtacaa ctcgctggct gatttgaaaa ataaaaaagg cagctacagc cattattccg
     5401 attacagcga cggtgccggg ttacaactgg cagccgatgt gcgtgaaaac gatctgctgt
     5461 cgtttgccgt taactggaaa gatgacgtac accgggaaaa aggtgcgccg cacgccgctt
     5521 acgatcgcta tgaagatcgt acctggtcgc tcgccagtga atatcaatgg gctgctgccg
     5581 ataatgtcga tgtcgtggct ggaatcagct atgactggcg cgatagcgta gaagcgaaaa
     5641 aacatgagaa agatggcagt atcacccatt atgacgacaa caatcagtca gcttttaact
     5701 ggcaggtgat ggggaaatac cactttgcca atgaagacac gctggcgctt tcgtactatg
     5761 accgcacacg ctttccgacg ctgaaagaac gctataccac gtccaaacct gcgtataacc
     5821 agatagcgat tgttaacccg cagctcaagc cggaacgcgc gcgcggggtg gatttaacct
     5881 ggaatggtgc cttcacgcac gactggggat ttgaggtcag cgtttactat aaccgggtga
     5941 gtgatgccat cctctcgcac aatatcgatg ccgataccat tcaaaatcag aacagcggca
     6001 cggtggatta cagcggtctg gatgccggta ttaaggggaa aatcagcaat atactggatg
     6061 taggattgag ctacgccctg atccacgctg atgccaaacg caaagacatc ggcaagataa
     6121 ccgatctgcc aacgcagaca atgaccgcat ggatgactct caaaccgtgg gagccgttaa
     6181 gcgtaacgct gtcggaagag gcgcgttcct ccagctacag caacagtgac ggttcacaaa
     6241 aagccgccgg ttttgcggtg actcacattc gagccgatta caccttaggt catggcttca
     6301 gcgttaatgc gtcggtcaat aacctgtttg ataccaaata tgcctacagt gaagggttta
     6361 ttgaagaagg tcgcaatttc tgggcaggta tcgagtatac gttctgatgt gaaagcggat
     6421 agcggcatct gtatcgctat ccgccttatg aaaatcttat tctcttcgca ggaggcggaa
     6481 tacgcccagc accaggatag cgcccaccac cgccaccagg aagctgtgca aattaaaacc
     6541 gctgatggag ccgccaatgc caaacatggt cgccagccag ccgccgacca ccgcaccgac
     6601 tatcccaaga atacaggtca ggaaaaatcc accaccatct cgccccggca tgattagctt
     6661 ggcgataatg ccggctatca ggccaaaaat aatccaggca ataattccca tgttcaggcc
     6721 ctcgtttcga tgaatcggac gcaagaaatg cttacgtcaa cgctctaagt ataggcaagg
     6781 gcgggatcga tgctgggcat atgtcggctg atttaagaac aacatgtaaa aaatattcgc
     6841 gcatttgtat taagttttgt taactgcgac cgataaacca aagacagttt gtcagtcagg
     6901 agtttttccg cgtgagtcat taccatgagc agttcctgaa acaaaatccg ttagccgtcc
     6961 tgggcgtgtt acgcgatttg aacaaagccg caattccttt gcgtatcagc tggaatggcg
     7021 ggcagctgat cagcaaaata ctggcaataa ctccggataa actggtgctg gatttcggca
     7081 gccaggccga agacaacatc gccgtgctaa aggcgcagca cattaccatt accgccgaaa
     7141 ctcagggtgc gaaagtcgag tttactgttg aacaactaca gcagagtgaa tacttgcagc
     7201 ttccggcatt tattaccgta ccgcctccca ccttatggtt tgtacaacga cgccgatatt
     7261 tccgcatcac cgccccactc catccgcctt atttttgcca gaccaaactg gcggataaca
     7321 gtacgttacg tttccgcctg tatgatttgt cgttaggcgg catgggcgca ttactggaaa
     7381 cagcaaagcc tgccgaatta cacgaaggca tgcgcttcgc tcagattgaa gtcaacatgg
     7441 ggcaatgggg tgtttttcac tttgacgccc agttaatctc catcagcgag cgcaaagtga
     7501 ttgatggcaa aaatgaaacc atcaccactc cccgtctgag cttccgtttt cttaacgtca
     7561 gcccgacggt ggagcggcaa ttacagcgga ttattttctc tctcgagcga gaagcccgga
     7621 aaaaagcgga caatgtgcgt gactgattta catcgcgtcc agtgcctgct caagtttgta
     7681 gatatagcgc ggagcctgcg gcgcaggatg atttcgcgct acgtgatcga ggaactcatc
     7741 agcatccaga tcgttgattt tgctgatcgc ctttttccga tctgacgaga aagtccgtag
     7801 cagcgcacct gccccgttag cgtatgacac caccagcgca tattgcagta ccttcggatc
     7861 ttcaatgcct gccagcgggc cggtttccag aatattcagg taagccgctc ccattgaaat
     7921 attacgctcc ggatttttca gctcgctggt cgtcggctca ccgctccagc ccatacgacg
     7981 atatacatca cgtccggagg ttgaagcttt tatctgcatc aaaccaatgg cattcgattt
     8041 actcaccgcg ttcggattac cacccgattc gatagcgata atcgccgtga tcaattgtgg
     8101 atcgacgccc caggctgcac cggctttctg gcttattggc atccactgca tcgcacgttg
     8161 caccggaact ttcgcgttcc acggcgggtt cgtatagtca tgctttgatg aacaacccgc
     8221 taataacaca atcaaaaagg caaaccatct caatttcact tcttctatcc ttatagcctg
     8281 gaatcgccgt tgaggtgaca acggcacacc taagcggcat gatatacatt tcgtttctgt
     8341 tgtcagcaag gaattgccat gtctctgttt cacttaattg ccccatcggg ttactgcatt
     8401 aaacagcacg ccgcgctgcg tggtatccag cgccttaccg acgcggggca tcaggtcaat
     8461 aacgtcgagg ttattgcccg tcgttgtgag cgttttgcgg gtacggaaac ggagcgtctg
     8521 gaagatctca attctcttgc cagactgact acccccaaca ccatcgtgct ggctgtacgc
     8581 ggcggttacg gtgccagtcg tttactggca gatattgact ggcaggcact ggtggctcgc
     8641 caacaacatg acccgttact catttgcgga catagcgatt ttaccgccat tcagtgcggt
     8701 cttctggcgc aaggcaatgt catcaccttt agcggcccca tgctggtagc aaactttggc
     8761 gcggatgaac taaatgcctt taccgagcac cacttctggc tggcgttacg caataaaacg
     8821 ttcaccattg aatggcaagg ggaaggcccg acatgtcaga ccgaaggcac gctgtgggga
     8881 ggcaatcttg cgatgctgat ttcactgatt ggtacaccgt ggatgccaaa aattgagaac
     8941 ggtattctgg tgctggaaga tattaacgag caccctttcc gcgtcgaacg catgctgttg
     9001 cagctctatc atgctggaat tttgccacgt cagaaggcga ttattctcgg tagctttagc
     9061 ggcagcacac ctaatgatta tgacgcaggt tacaacctgg agtcagtgta cgcgtttttg
     9121 cgttcccgcc tgtcgattcc actcattacc ggcctcgatt ttggtcatga acaacgcacg
     9181 gttacgctgc cgttgggcgc acatgccatt ctgaataaca ccgaggaagg cactcagttg
     9241 acgattagcg gtcatcctgt tcttaaaatg taaaaaaatc acgcttcagg gctaagtaaa
     9301 acgctgtctc tgccgctaat atggtagggt ttatatcaga gacagcgtaa tcaggagtaa
     9361 ccgaccgttg gatgccacaa caataattag ccttttcatc ttaggatcca tccttgtcac
     9421 cagcagtata ttacttagtt cattttcttc ccgtcttggc attcctattc tggttatttt
     9481 tctggcgatc ggcatgttgg cgggggttga tggcgtcggc ggtatcccgt ttgataatta
     9541 ccccttcgcc tacatggtaa gtaacctggc gctggcgatt attttgctcg acggcgggat
     9601 gcgcactcag gccagttcct ttcgtgtggc gttaggaccg gcactgtcgc tggcgacgct
     9661 gggcgtactt atcacctctg gtttaaccgg catgatggcg gcgtggctgt ttaatcttga
     9721 tttgattgaa ggattattaa tcggcgctat cgtcggctcc accgatgctg cagcggtctt
     9781 ttctctgctg ggtggtaagg ggcttaacga acgtgttggc tcgacgctgg aaattgaatc
     9841 cggcagtaat gatccaatgg cggtctttct gacgattact ctgattgcga tgatccagca
     9901 gcatgaaagc agtgtcagct ggatgttcgt ggtcgatatt ctgcagcaat ttggcctcgg
     9961 gattgtcatc gggcttggcg gtggttattt actgctgcaa atgattaatc gcatcgccct
    10021 gcccgccgga ttatatccat tgctggcatt aagcggcggt attttaattt ttgctttaac
    10081 cacggcgctg gaaggcagcg gtattctggc tgtttatctg tgcggttttc tgctgggtaa
    10141 tcggccgatt cgcaaccgct acggcatcct gcaaaatttc gacggcctcg cctggctggc
    10201 gcaaatcgcc atgttcctgg tgctggggct attggttaac ccaagcgatc tgctgcctat
    10261 tgccattccg gcgctcattt tgtccgcatg gatgattttc ttcgcccgtc ctctttcggt
    10321 atttgccgga ttgctaccgt tccgtggctt caatctgcgt gagcgcgtgt ttatcagctg
    10381 ggtaggatta cgcggcgcgg tgccgattat tctggctgtg ttcccgatga tggcggggct
    10441 ggagaatgcg cgactgttct ttaatgtcgc cttctttgtg gttctggttt cactgctatt
    10501 gcagggaaca tcactctcgt gggcggcgaa aaaagccaaa gtggtcgttc cgccagtggg
    10561 acgtccggtg tcacgcgttg gcctggatat tcatccggaa aatccgtggg agcagtttgt
    10621 ttatcaattg agtgccgata aatggtgcgt gggcgcggca ctgcgtgatt tgcatatgcc
    10681 aaaagagacg cgtattgcgg cactgtttcg tgataaccag ttgcttcatc ccaccggcag
    10741 cacccgactg cgcgaaggcg atgtgttgtg tgtaattggt cgggaacgcg atctcccggc
    10801 gctcggtaaa ctgttcagcc agtcgccgcc ggtcgcgctg gatcaacgct tctttggtga
    10861 cttcattctc gaagccagcg ccaaatatgc tgatgtggcg ctgatatatg gtctggaaga
    10921 cgggcgagaa tatcgcgata agcagcaaac gctgggtgaa atcgtccagc agttgttagg
    10981 cgcggcaccg gttgtcggtg accaggtaga gtttgccggg atgatctgga cggtggccga
    11041 gaaagaagat aatgaagtgt tgaagattgg tgttcgggta gcggaggaag aagccgaatc
    11101 ttaagtttaa tgtgtttcgt aacgctggat acgacgctgg tcgcatccag cgataacaag
    11161 ttacaccgtc acaaccggga cgcgtaacgc cagcgcgcac atcaactcat agcctaccgt
    11221 tccggcagcg gcggcgacat catcaatttt gatctccttg ccccacagct caactggcgt
    11281 accaataccc gcttgcgggc aaggcgttaa atcgaccgcc agcatatcca tcgagactgt
    11341 ccccaccgtc atggtgcgaa cgccgtccac taaaacaggg gtaccggtag gcgcgtggcg
    11401 cggataaccg tcggcgtatc ctgcggcgac aatgccgatt cgctgttcat cgcgcgcagt
    11461 atagcgaccg ccgtagccca cacgctcgcc cgcttttagc gtctggacac caataatctc
    11521 actgcttagc gtcatcaccg gacgtaatcc ggtattggcg atatcacgcc actgaccgga
    11581 cggcgaagca ccatacaaaa taatgccagg ccgaacccag tcaaaatgcg cttcctggtg
    11641 ccacagagtc gccgccgaat tagacaacga acgccgacac tccagccctt ccgccgcctg
    11701 ctcaatacgc gccatcgcgc tggaaattcc atcaggatgt tccgcttcgg caaaatgcga
    11761 catcagggtc atttcgccaa cattcgccat cgcccgcaac tgctgccaga cggtaagcac
    11821 gcgatcgggc tggaagccca accgattcat cccactgttc actttaagat aaatatccaa
    11881 cggtgctttt agccgcgcat tttgcagtgc tttgagctgc cagttgctgt gtacgcaggt
    11941 ggtcaggcgg tgctggtcat aaatctccag atcctgagca tggaaaaatc cttccagcat
    12001 caggatcggt cctttccagc cgcgctcacg taacgttatt gcctcttcca ggttaagcaa
    12061 tgcaaagcca tcggtagccc cgagcgcgct ccagatacgc tcaataccgt gcccgtaagc
    12121 gttcgctttt accaccgacc agacgcgcgc gtgcggcgcg gcctggcgga caatggacag
    12181 attctgtttt aatgcctgca gatcgaggct ggcctgtatc ggacgggtca tctcgtttcc
    12241 ttagctgtgt gcgccatgta aatggcccgg gcgtgatggc gtaaatccac ggctgtagcg
    12301 cgctacgctt agagcttcat acgggatcgc tggcgtgcgc cccgagagca gatcgcttaa
    12361 caactgaccg gaaccgcaag ccatcgtcca gccgagcgtg ccgtgaccgg tattcaacca
    12421 cagattttta aagcgtgtac gcccgacaac cggcgtgccg tctggcgtca tcgggcgcag
    12481 gccagtccag aaagccgcct gatcaacatg accgccgcgt ggatagagat cgcgaaccac
    12541 catctccagc gtttcacgac gcggttgcaa cagctcggtg ttaaaaccaa caatttccgc
    12601 cattccgccg acacgaatgc ggttatcgaa acgggtaatg gcgattttgt aggtttcatc
    12661 aagaatagtg gataccggcg caccatcttc ctgcgcaatt ggaatggtca gcgagtagcc
    12721 tttcagcgga tacaccggaa tatcaacaat gcctttgagc atcgccgtcg agtaagaacc
    12781 aaacgccatc acatacgcat cggccttaat cacttcatca ccacacttca cgccgtagat
    12841 ttgctcgccg tcgcaaagca gttggtcaac gggcgtatta aagcggaatt taacccccgc
    12901 ctgctccgcc atccgcgcca gattctgggt aaacagctgg cagtctccgg tttcatcatt
    12961 gggcagttgc aggccgcccg tcagtttgtg cgctacttct gccaacgcgg gctccacttc
    13021 cgccaggcgg ctggattcca gcagctgata cggtacgcca gcatcttcca gcacggcgat
    13081 atcgcgggtc gcgttttcat actgttgttc ggtacggaac agttgcagcg tcccaccctg
    13141 acgcccttca tactgaatat tggtttcggc gcgcaatgct ttcaggcaat cacggctgta
    13201 ttccgccaga cgtaccatcc gccctttgtt ttccatgtag tggctggtgt cgcagttacg
    13261 taacatttgc cacatccatt tcaactggaa ctgcgtaccg tcgagacgaa ccgccagcgg
    13321 cgcatggcgc tggaacatcc atttaatcgc ttttaaaggc acacctggtg ccgcccacgg
    13381 tgcagcatat ccgggggaga tttgcccggc attcgcggca ctggtttcca gggcggcccc
    13441 cggctcccga tcaatgacgg tgacctcatg tcctgcctga tttaagtacc aggcgctggc
    13501 aacgcctacc acaccacttc ccagtatgac aactcgcata gccactccga
//
LOCUS       DAJOTB010000125        13515 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJOTB010000125 DAJOTB010000000
VERSION     DAJOTB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.125"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            74..997
                     /locus_tag="OHN28_003664"
     CDS             74..997
                     /locus_tag="OHN28_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2298982.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1001..1819)
                     /gene="nlpA"
                     /locus_tag="OHN28_003665"
     CDS             complement(1001..1819)
                     /gene="nlpA"
                     /locus_tag="OHN28_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCQ2298983.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2041..2334
                     /locus_tag="OHN28_003666"
     CDS             2041..2334
                     /locus_tag="OHN28_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2298984.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2375..3565)
                     /gene="nepI"
                     /locus_tag="OHN28_003667"
     CDS             complement(2375..3565)
                     /gene="nepI"
                     /locus_tag="OHN28_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCQ2298985.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            3799..4152
                     /locus_tag="OHN28_003668"
     CDS             3799..4152
                     /locus_tag="OHN28_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2298986.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4136..4459
                     /locus_tag="OHN28_003669"
     CDS             4136..4459
                     /locus_tag="OHN28_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCQ2298987.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(4575..5027)
                     /locus_tag="OHN28_003670"
     CDS             complement(4575..5027)
                     /locus_tag="OHN28_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCQ2298988.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5080..6414)
                     /gene="adeQ"
                     /locus_tag="OHN28_003671"
                     /gene_synonym="yicO"
     CDS             complement(5080..6414)
                     /gene="adeQ"
                     /locus_tag="OHN28_003671"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCQ2298989.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6589..8355
                     /gene="adeD"
                     /locus_tag="OHN28_003672"
     CDS             6589..8355
                     /gene="adeD"
                     /locus_tag="OHN28_003672"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCQ2298990.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8402..9793)
                     /gene="uhpT"
                     /locus_tag="OHN28_003673"
     CDS             complement(8402..9793)
                     /gene="uhpT"
                     /locus_tag="OHN28_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCQ2298991.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9931..11250)
                     /gene="uhpC"
                     /locus_tag="OHN28_003674"
     CDS             complement(9931..11250)
                     /gene="uhpC"
                     /locus_tag="OHN28_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCQ2298992.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(11260..12762)
                     /gene="uhpB"
                     /locus_tag="OHN28_003675"
     CDS             complement(11260..12762)
                     /gene="uhpB"
                     /locus_tag="OHN28_003675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCQ2298993.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(12762..13352)
                     /gene="uhpA"
                     /locus_tag="OHN28_003676"
     CDS             complement(12762..13352)
                     /gene="uhpA"
                     /locus_tag="OHN28_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCQ2298994.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         3627 a         3634 c         3290 g         2964 t
ORIGIN      
        1 tccctcaccg tcatcggtag tggtatcctt aacgtatatt gatgaaaaga caaaacagta
       61 tgcagaggga aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt
      121 gttgtgggga agttcagggg tctgcgcgca atacatcatg gagcaaagcc agatgtcgtc
      181 gcagtttttg actatgacgc gtttgatatt cgccggattg attctgctga cgctatcatt
      241 tgttcatggt gataaaatct tttctattat taacaatcac aaagatgcca ttagcctact
      301 gattttttcc gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa
      361 atcgaacgca gccacggcaa cggtgctgca attcctctca ccgactatta tcgtcgcctg
      421 gttctcactg gtgcgtaaat cccgccccgg catcctggtt ttttgcgcta ttttgacatc
      481 gctgatcggg acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc
      541 tgccgcgtta ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc
      601 ctcaacgctg attgctcgct atggcacgtt accggtcgtc ggctggagta tgctcattgg
      661 cggtctgatc ctgctgccct tttatgccag acaagggaca aactttgtcg ttaacggcag
      721 tttgattctg gcgttttttt atttggtggt cattggtacg tccctgacat ttagtctgta
      781 cctgaaagga gcacaattaa ttggtggtcc aaaagccagc attttaagct gtgcagaacc
      841 attaagtagc gcactgctct ctttgctgtt gctggggatc accttcacat tgccggactg
      901 gctgggaacg ctgctgattc tgtcatcggt gattttgatt tcaatggatt cccgccgccg
      961 cgccagaaaa ataaatcgtc cggcgcggca tgagtgagaa ttaccagcca ggcaccgcgc
     1021 ctccgttaaa gatggtttct gccgctttag cgacttcggg tgattgataa gattgcagaa
     1081 attccttcac gttttctgca ttcttattat cttcccgtgc caccagaata ttcacatacg
     1141 gcgaattttt atcttcaata aatacgctgt cgttaaccgg agaaagtcct gtttgctgga
     1201 tgtaagtggt gctgataatc gctacatcca ctttcgggtc gtccagtaca cgtggcaact
     1261 gtgccccttc cagctccatt atctgcaaat ggcgtggatt atcggttata tccaacgcgg
     1321 taggtaataa cccttttccc tctttcagag taattagctt ctctttttgc agcagtaaaa
     1381 gcgcacgacc aaggttagtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt
     1441 gcgccacggt tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca
     1501 ccgctaccag tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga
     1561 agacgttcgc atcaagctcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa
     1621 atcctaccag ttcaacatcg aggccatatt tctcttttgc taccttttta gcgacttccg
     1681 cgacatcttg ttctgcgcca ttaataacgc caactttaat atgcttttca tcgctactgc
     1741 tctggtcgca acctgccagc agaactcctg ccagtaataa tgcggccccc gcccgtagat
     1801 gatgtgttgt cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta
     1861 aggtcgctgc agtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg
     1921 aatataaagt ctggcacttc ttactgacca cgcaaacacc tttcgtacag catgatgaga
     1981 gcggcaaaag caaactacac ttatgcttca ttcgatgcac atttgcagaa ggttgccgct
     2041 atgaagccaa cgatgctact catgattacc atattcctga tttttccagc catttctcag
     2101 gccgagtcgc cattcagttc cctacagtcg gcaaaagaga aaaccacggt attgcaagat
     2161 ttacgtaaaa tttgtacccc gcaggcatcg ttatcagatg aagcgtggga aaagttaatg
     2221 ttgtctgatg aaagcaataa acaacacata cgcgaagcca tcgtagcgat ggaacgtaat
     2281 aatcagagca attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacgg
     2341 caacaaccac tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg
     2401 cagtcaccaa caatgcagtc agcaacatca atgtgccgga caacatcagc ggcgaagtca
     2461 gaccaatgtt atccaacgca taaccgccga ttgccgcacc gcaggtattg gcaagctgaa
     2521 taacggccac ctgaatagat ccggcttttt ctgcctgatc ggccagtgag cgagtgatcc
     2581 acgttgacca gccaacggga attaaagcaa aggtcagccc ccagataatc gccacaccag
     2641 tagcaacaat tttatcgctt ccccacagcg tcagtaccag cgcgctcagg gcgagcacaa
     2701 acggtgcgcc cgctaaggcc agtttcaccg aacgtttgag aataaacgac gaaagcgatg
     2761 tcccgacaaa gctggcgata ccaaaactca gcagcaccag ggttaagcca tcaacaccga
     2821 atcccgccag attcatatac actgggcgaa tataagtgaa aaaagcaaac tgtccggcaa
     2881 aggacataaa gatggcgatc atcccggcca tcactcccgg acgttgtaat aagcggaaag
     2941 tattttgttt ctgatgcgaa ggttcgcctg gcagcgaagg cagtgatttg ataatccaga
     3001 aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct
     3061 cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga
     3121 tcaccgacag cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg
     3181 acatcgccca gaatccgccc agcgccagcc ccagacaggc gcgaccgatt aacaacagac
     3241 tgaatgagtt ggcaaaggaa accagcaaac aggaaagcgt cagcaaaacg gcgaacagaa
     3301 tgacaacgta gcggcggtca gtggcctgaa tggtctgggt aataaacaaa ctggcaaaca
     3361 tcgccacaaa agcggttacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat
     3421 cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataata agacaggcaa
     3481 cgcaaaatgc caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc
     3541 ggttttcagc aataaattca ctcatggtgt tacccgtggc atgtttcaga gggaaagccg
     3601 accactttaa cattgaaatg tgtgacgtat ttaacgtttt tgcaactttt acggtgttgt
     3661 gggaaaagtt cgaggctgct cgaagaaata aaaatcatag ggcaaggtga tatgcatttt
     3721 cgcgattagg ttgggaataa gggataaagt attgcacttg gtgatatact ttaataacag
     3781 gatctcaacg atatgcgcgt gtttaaaacc aaatggtttg caagggaagc caggtctcat
     3841 gcaataacag atgaagagtt gtgccgggcc attttagaaa cggaacaagg taaagctgat
     3901 gtacttggtg gtggcgtttt taaaaagcgc cttcaccgaa atcgtgagcg tgcaattatt
     3961 ctggcgaaag ggggaagcaa ttggttttac acgtttttat atgcaaaaca ggatatgtcg
     4021 aacattaata gtcaggaact ggctgggttt cgtgaacttg caaagcatta tgccttcctg
     4081 acgaaagccc aactgacagc aatgattaac actaaagaac tgacagagat ctgctatgac
     4141 tgcaaaaact aaatttaaaa gtcctgcatt tgaagctatt cacagcgcag cggctggatt
     4201 gtccagagtc gatgctattt ctgccgaaac gatgcgtact tttgacaaag cctgcctgac
     4261 cagcgtgcaa gacttacaac ctgttgagat aaaagcactg cgggaaaaat taaaagtgag
     4321 tcagcccgtc ttcgctcact atctcaatac cagcgtttca actgtacaaa aatgggaaag
     4381 tggcgctaaa cgccccaatg gcatttcatt aaaactgctc agcattgtgc aaaaacacgg
     4441 gctggaggtg ctgctgtaaa tagaacgtag ataatgcgtt cacctactgt caattccgat
     4501 accggatgcg acgtacacgc cttatccacc caccaggtca ggcgcggtct gtaggctgat
     4561 aagacgaagc ggttttaatg cagccagtga acgccctcgg caggctgaaa gaacgcgtta
     4621 tagcgcatct gaaaagcatt gatatcctgc atttcaggtt cggtttcgcg cagaaaaccc
     4681 agcgccagtc tgacctgagc atcggtaatc ggcgcggcaa ggcgggcttt ttgtaaaatc
     4741 cgatgccatt gcagcaggtt ctgttcgcta ccatcgacaa tgacaatttg ccagatgagc
     4801 aacacctgcg cggcgttttg caggtgcgac tcatccgggc gatgcaaata acgtaaaaat
     4861 tcgtcaccgg ctgactttcc catttgatcg accatcgact ccacgggtac gacatcgatg
     4921 ttcagccgat cgctgagacg gcgaatcgct tttctggccc cggatgtcag cacccgaaaa
     4981 ccaaccacaa acactaccgc cagcgtggcg agcattatcc agatcatgag ttctcctgcg
     5041 taatcagaac tcatgatatc tggaaaccct cgccagttac taatccacca gaataatctt
     5101 cagtgcaaac agagctgcga ccaccaccac acacaggttc agatcgcgcc agcgcccggt
     5161 gcacactttc atgatgcagt acgacataaa gcctagtgca atcccttcgg tgatcgagaa
     5221 cgtaaagggc atcatcaccg tggtaataaa cgcaggcacc gattcggtaa aatcatccca
     5281 gttaacgcgt gccaggctcg atgtcatcaa tacgccaaca aagattaacg ctccggcggt
     5341 tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac ataaccagca gaaacataac
     5401 gccgacaaca accgcagtca gccccgtgcg accaccgaca gccacacctg aagtactttc
     5461 aatataggcg gtaacagacg aggtaccgat aaacgcaccc gccaccgaac tgatgctatc
     5521 aacatacagc gccttattca tattggggaa tttaccgtta ctatctatta agccagcttt
     5581 atcagtcaca ccaattaatg tccctgatga atcaaataag ttgatcagca taaaggagaa
     5641 aatgataccg gcgagttcaa gtgataacgc accgctcaaa tccacttcac caatgacgcc
     5701 gctaatatca ggcggaatgg aatagacacc gctaaaatga acatcaccga aaaataatcc
     5761 acagcaagac gtcaccacaa tagaaacaag caccgcggca tgaaaatgac gtgatgacaa
     5821 cacagttata ataaaaaacc ctaaaatacc taacaacacg ccgtgagaac ttaaatcgcc
     5881 aatcatcacc agggtgtctt tattggcgac aataacgcca gtatttttta atcccattaa
     5941 ggcgataaat aatccgattc cgctggtgat accaatacgt aaacttaatg gaatgttgga
     6001 gatcatccag taccggatac gaaacagcgt gagcaaaaat agcccaactg cgccccagaa
     6061 tatcgcgccc atcccggtct gccaggagat acccatcgcc cccacgacca cgaaggcaaa
     6121 gaaggcgttc aggcccattg ccggagccag tgccacgggt aagttagcaa atacccccat
     6181 cgcaatactg ccgataccgg caatcaaaca ggtggtaaca aacaccactt ttgggtccat
     6241 ttgtgccgcg ccgaggattt gcgggttcac aaaaacgatg tacaccatgg ttaaaaaagt
     6301 ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca tgctgaggta gtttaaataa
     6361 tcgcgaaagc gtccctgatt cattactcac gtaatcggta ttgtcattat tcattttttt
     6421 gtccattgga ggagatttaa tcccttgctt ttgtaaaaca aatgacaaca acggttcagt
     6481 gataattatc acatttcaat tgcacattaa tggatattct ttaataatct cgcgacgttt
     6541 ctttatgata aataataatc aaattgataa aatcaaaatg agaaaaatat gaataattct
     6601 attaaccata aatttcatca cattagccgg gctgaatacc aggaattgtt agccgtttcc
     6661 cgtggagacg ctgttgccga ttatattatt gataatgtct ctattctcga cctgatcaat
     6721 ggcggagaaa tttccggccc aattgtgatt aaaggacgtt acattgccgg tgttggcgca
     6781 gaatacgctg atgctccggc tttgcagcgg attgatgccc gcggcgcaac ggcggtgcca
     6841 gggtttattg atgctcacct gcatattgaa tccagcatga tgacgccggt cacctttgaa
     6901 actgctaccc tgccgcgcgg cctgacgacc gttatttgcg accctcatga aatcgtcaac
     6961 gtgatgggag aagccggatt cgcctggttt gcccgctgtg ccgaacaggc aaggcaaaac
     7021 cagtacttac aggtcagctc ttgcgtaccc gccctggaag gctgcgatgt taatggtgcc
     7081 agttttaccc ttgaacagat gctcgcctgg cgggaccatc cgcaggttac cggccttgca
     7141 gaaatgatgg actaccctgg cgtaattagc gggcagaatg cgctgctcga taaactggac
     7201 gcatttcgcc acctgacact ggacggtcac tgcccgggtt tgggtggtaa agaacttaac
     7261 gcctatattg ctgcgggtat tgaaaactgc cacgaaagtt atcagttgga agaaggacgc
     7321 cggaaattgc aactcggcat gtcattgatg atccgtgaag ggtccgcagc ccgcaatctc
     7381 aacgcgctgg caccgttgat caacgaattt aacagcccgc aatgcatgct ctgtaccgat
     7441 gaccgtaacc cgtgggagat cgcccatgaa ggacacatcg atgccttaat tcgccgcctg
     7501 atcgaacaac acaatgtacc gctgcatgtg gcatatcgcg tagccagctg gtcgacggcg
     7561 cgccattttg gtctgaatca cctcggctta ctggcacccg gcaagcaggc cgatatcgtc
     7621 ctgttgagcg atgcgcgtaa ggtcacggtg cagcaggtac tggtgaaagg cgagccgatc
     7681 gatgcacaaa ccttacaggc ggaagagtcg gcgagactgg cacaatccgc cccgccatat
     7741 ggcaatacca ttgatcgcca gccagtttcc gccagtgact ttgccctgca atttaccccc
     7801 ggaaaacgct atcgcgttat tgaggtaatc cataacgaac tgatcacgca ctcccgctcc
     7861 agcgtctaca gcgaaaacgg ttttgatcgc gatgatgtgt gctttattgc cgttcttgag
     7921 cgttacggac aacgactggc tccggcctgt ggtttgctcg gcggctttgg cctgaatgaa
     7981 ggtgcgctgg cggcgacggt cagccatgac agccataata ttgtggtgat cggtcgtagc
     8041 gcagaagaga tggcgctggc ggtcaatcag gtgattcagg atggcggcgg gctgtgcgtg
     8101 gtccgtaacg gccaggtaca aagtcatcta ccgttgccca ttgccgggct aatgagcacc
     8161 gacacggcgc agtcactggc ggagcaaatt gacgccttga aagccgccgc ccgtgaatgc
     8221 ggtccgttac cggatgagcc gtttattcag atggcgtttc tttccctgcc agtgatcccc
     8281 gcgctgaaac taaccagtca ggggctgttt gatggtgaga agtttgcctt cactacgctg
     8341 gaagtcacgg aataataaaa aaaagcccgg cgtcatgccg ggcaaaagtc accagttacg
     8401 tttatgccac tgtcaactgc tgaatttttt tctcgcggcg gattttgcgt tcttccatca
     8461 ccgccactat cgccatcagg cagatacaac caatcgcggc gatatccagc gcggcgaagg
     8521 tgcctgccca gccggtaagg ccaaataccg gcgtcccatc ggcaatcatt cccagaccta
     8581 acttggcaaa gctgtcacca atcaggtaag cgaaggtgcc tttaatacca tcggcagcgc
     8641 caatcgcttt tttaggtaca aagccaacag ccgccacgcc aatcaacagt tgcgggccaa
     8701 agaccaggaa acccaacgca aagagagaag ccaggtagat atattggttg ctggcgtgtt
     8761 gatacacacc gagcgtggcg ataatcagcg ccagcgcgat acaggccacc aggccacggc
     8821 gaccgttcgc caggtcagag agccagcccc acagcagcgt accgaccagc gcaccagctt
     8881 caaacagcgt aaagccctga atcgccaccg ctttagagag tttcagttcc tggaacgcat
     8941 acacggttga ccactggtcg ataccaatac gcaccacata gaggaaaatg ttggcgaagc
     9001 acagcagcca gatcactttg tttttcagca catactcaac aaagatctgc cacttggtca
     9061 tatcggtaga ttctgtctct ttatcctctt cactgatctc ctcgccaaac agctcttcag
     9121 ctttgccgag gccataagat tccggggagt cgctgccgta acgcagaccg ataaaaccaa
     9181 caatcagcgc gataatcgac gggaaaataa acatgccgat gacatgacca tcgaacaggt
     9241 aatttgcgcc gaacagcgcc acacctgctg cgcctgcacc accaaggttg tgagaaatgt
     9301 tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt ccatttggtg atggtggagt
     9361 aactgcatga accgccggta ctctggaaaa agccgctcaa ggcgtagaag gcaatcatca
     9421 ggaacaggct aaccgagccg ctgcccatac tggcactgaa gcccagcata caaatggcag
     9481 acaggatcag catgaacggc aggaattgtt tggtgttttt gccgtcggcg tagtaagaaa
     9541 ccagcgtttt acccacgcca taagtgatgg agaagcccag gccgatcatc cccagctgcg
     9601 tcatgctcaa cccgtaggtc gagatcatgt cgttctgcgc gatgttaaag ttcttgcgaa
     9661 tcaggtacat cgtaaggtag ccgataaaga ccaccaggta ggattgcatg aacggtttga
     9721 accacatttt gcgccgcact tcgagcggaa ggtccagggt cggcttgcga acctggttta
     9781 agaaagccag catgggttac tcctgaaatg aatacctgcg tgaggcatgc attgtaaaaa
     9841 tcagcagcaa atcaggcctg agatagcgtc caggcaaaac ctggaaaatt tcttagtttt
     9901 gccggatatg aagtgaaaag gtgagatgca tcacgcttct cgcggtgtct gggcgttcaa
     9961 aaagggcagt aacagcagtg cggaaatccc ggcggcgatg gcgataacca caaaaaatcc
    10021 gctccagtgc caggtatcga gtactttcgc cagcggccaa ccagcaagcg acgcaccaag
    10081 ataagcaaac aagccgacaa accccgtcgc cgcccctgcc gcctctttgt gggaacactc
    10141 tgccgccgcc atgccgatta acatctgcgg gccaaagaca aaaaaaccaa tggtgaagaa
    10201 gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc cacagcgagc cgactgaaag
    10261 caaaattccg gcggcgaaaa tcaaattcat cggcccgcgg ttgccgttaa acagtttgtc
    10321 cgaaccccaa cccgctacca gcgcaccgat aaatccgccc agttcaaaca ttgtcactgc
    10381 cgtattcgcc gtgaccagat cgacgcccag cgtctcggac atatacaaat tgccccagtc
    10441 gttgatcgcc gcccgaacca catagaccag cacatagcaa aacgaaagca gccagatata
    10501 cggattcagc aacacatatt tggtgaggat ctctttacgc gtcagccctg ccccttcttg
    10561 ttgttgagca atctccagtg cgtcgtgccg ccattcacca actgccggta aacctaacgc
    10621 ctgcgggcga tcgcgcagcc gccagcagag aaaaatcccc acgacgatcg ccatacaacc
    10681 cgcaatcatc atcccggcac gccagccgta atgcagcgcg ctcgctgcca tcacaatggg
    10741 aatgagtgcg ccgccgacgt tatgcgccgt gttccataat gcccaccaac cgccgcgctc
    10801 ggtacgagaa taccaggcgg ttaacagacg cgcacacacc ggtgaacccc agccctggaa
    10861 aaaggcgttc aacacccaga gcacggcaaa cgcccatagc gacgtcgaga agccaaacag
    10921 aatgttgata atgcccgtgg caataagccc tatccccata aaataacggg catttgagcg
    10981 atcgctgaca atgccggaga caaacttcga cacaccgtag gtaatgtaaa acagggtcgc
    11041 taacaggcca atatcgctac ggctgagcac gccgttagca aggatttctg gtacggcgga
    11101 gttaaaactt ttacgcgtga agtaaaacag cgcgtaaccc agccagatgg tcagcagaat
    11161 atgccgacgc cagtagcgat agcgggcatc aatttcatgt ttatcagtca ttaatggcgc
    11221 atcggcaggc gctttcagaa acggcaacat catcgaacct tagacataac gttgaggtag
    11281 agaaacgctg acacgcgtgc cgtgcaggca ggagatggtc aatgtgccac ccagcgccgt
    11341 tacgcgctcg cgcattccgg taaggccaaa accgtgttgc ccggaatctg gcggtaaacc
    11401 gctgccatcg tcttcaatca ccagcattaa ccgctcatca tgctgccagc cttgcagcgt
    11461 gaccgcgctg gcatcggcat gtttcacaat gttgttcaac ccttcctggc agacgcgaaa
    11521 tagcgtcacg cgctggtttt cgcttaacgc tgattcatcg attcgccatt cgagatgact
    11581 gacaatgccg cgcccttcca gctccatttc ccgcatcagt gagcggatgg cctgctccag
    11641 ggtgagatca tccagctggc gcggacgtaa ccgccctaac aagcggcgca ccgcgtcgta
    11701 aacgcccaac gacaactgtt cgatgagctg cccgctctgt ttcacgctgg cgttatccgc
    11761 cgcgagccgc tggacaatgc cagcctgagt acgaatagca gtgatggtct gaccgatatc
    11821 atcgtgcagc tctcgcgcca catcgcggcg cacgctctct tcggtttcca gcaaccgttc
    11881 agcgagatgc tgattgcgcg ccagttcctt ttgcagcgac tggttaagtt cacgcaaccg
    11941 ctggatgcca gcgcccagta acaaccctgt cagactttgc accagcagcg agagcaataa
    12001 atccaccgga tgatcgcgcc aggtttgact ggcgatcagc gcgatggcgt tcatcaatgt
    12061 cgcaatcagc gctccttgcc aaccatagtg ccaggccagc gcgataatcg gcagcgccag
    12121 acagaatggc gtaaagcgcg acagttcgtc tggcaatccc aactggagcc agagactgat
    12181 aacaaacagc agcaagtacc agaccagatg tcgcccgcgc cagttgattg gctgagaaac
    12241 cagtgacggg ccgagcggca gccaggtgtt attggcgaga tagtgccaga acaccagaca
    12301 tatcggggcc agcgtcaggc cgccagttaa agtcagcagc agcgcattcc acgactcttt
    12361 gccgtgccaa agccagggca acgactgcaa caacgccgcc gccgttaacg ccgcaccctg
    12421 taacagcaag gtgcgccagt cacgttgatg gcgatagcgc gagatcagcg ccacgggcaa
    12481 taacgtcagt aaactaccga tcatcaataa ggagaaatgg gttaaaccga ccgcctgcat
    12541 tagccagtaa atcagcagcc actccgcacc cagtagcacc ggccagtatc cgcgcgggca
    12601 ttgcagcatt agccctagcc gcaggccaaa cggaaataac agcaccgcca tatcagggcg
    12661 ctcaaccagg tgcaggctga tactccacag gcaaaaccat gcggcagaga agataaaaaa
    12721 gcaggcaata acggtaatta aacgggagaa caacgtcttc atcaccagcc atcaaacatg
    12781 cggcgcgcca gttcaacgtc gttactgacg cccagttttt ccatcagatt ggcgcgatgg
    12841 acgtgtactg ttttcggcga caagcccagt tcggcggcaa tctctttcac cgccattcct
    12901 tgcgccagtt tttctgccac ctggcgttcg cgtttggtta gcgggtcctg gcgaccagat
    12961 gccagtttaa tggcaatatc cggcgtcaga taacagccgc cagtggcaac cgtatgcacc
    13021 gcagcaatga gttcatccgg gctacagcgt ttggagagaa agccgcgcgc ccccgcgtta
    13081 agcgcctgct caaccagcgc cggactgtcg tgaacggaga gcataattgt cgccatgcct
    13141 ttcggcagct ggcttagaag ctccagaccg gagatatcag gcatggagat atcgcaaata
    13201 cacacctgca caccgcgccc cggcagcccc gccagcgcct cgcgccccga accaaactcg
    13261 gcaactacct gcaaatcagg ttccagcccc agcagctgcg caaagccgga gcggacgata
    13321 aggtgatcgt ctataagggc aacggtgatc atggtcttgt cctggcgggt aataaaacgc
    13381 gcttacctta acgataagcg cgatgttgtt caagccttga gcggttactg ccaaaagctg
    13441 cgctcttgtt gcttgtggaa agaagtgatg ccttcaagga cttgtatatc gactaaccat
    13501 taaaaatgct ggcgg
//
LOCUS       DAJOTB010000126        13468 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJOTB010000126 DAJOTB010000000
VERSION     DAJOTB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.126"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..323
                     /locus_tag="OHN28_003677"
     CDS             <1..323
                     /locus_tag="OHN28_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HCQ2298995.1"
                     /translation="IISGLLLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVL
                     LFVLLLDILGFVICSFLLLFILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPE
                     GILF"
     gene            333..1820
                     /locus_tag="OHN28_003678"
     CDS             333..1820
                     /locus_tag="OHN28_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HCQ2298996.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFSIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            2004..2768
                     /locus_tag="OHN28_003679"
     CDS             2004..2768
                     /locus_tag="OHN28_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2298997.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(2882..3580)
                     /gene="thiQ"
                     /locus_tag="OHN28_003680"
     CDS             complement(2882..3580)
                     /gene="thiQ"
                     /locus_tag="OHN28_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCQ2298998.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(3564..5174)
                     /gene="thiP"
                     /locus_tag="OHN28_003681"
     CDS             complement(3564..5174)
                     /gene="thiP"
                     /locus_tag="OHN28_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCQ2298999.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQVLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(5150..6133)
                     /gene="thiB"
                     /locus_tag="OHN28_003682"
     CDS             complement(5150..6133)
                     /gene="thiB"
                     /locus_tag="OHN28_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCQ2299000.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(6164..6263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6297..7952)
                     /gene="sgrR"
                     /locus_tag="OHN28_003683"
     CDS             complement(6297..7952)
                     /gene="sgrR"
                     /locus_tag="OHN28_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCQ2299001.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLTEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            8041..8172
                     /gene="sgrT"
                     /locus_tag="OHN28_003684"
     CDS             8041..8172
                     /gene="sgrT"
                     /locus_tag="OHN28_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCQ2299002.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(8279..8884)
                     /gene="leuD"
                     /locus_tag="OHN28_003685"
     CDS             complement(8279..8884)
                     /gene="leuD"
                     /locus_tag="OHN28_003685"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCQ2299003.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(8895..10295)
                     /gene="leuC"
                     /locus_tag="OHN28_003686"
     CDS             complement(8895..10295)
                     /gene="leuC"
                     /locus_tag="OHN28_003686"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCQ2299004.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(10298..11389)
                     /gene="leuB"
                     /locus_tag="OHN28_003687"
     CDS             complement(10298..11389)
                     /gene="leuB"
                     /locus_tag="OHN28_003687"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCQ2299005.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(11389..12960)
                     /gene="leuA"
                     /locus_tag="OHN28_003688"
     CDS             complement(11389..12960)
                     /gene="leuA"
                     /locus_tag="OHN28_003688"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCQ2299006.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIDKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(13052..13138)
                     /gene="leuL"
                     /locus_tag="OHN28_003689"
     CDS             complement(13052..13138)
                     /gene="leuL"
                     /locus_tag="OHN28_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCQ2299007.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         2983 a         3496 c         3582 g         3407 t
ORIGIN      
        1 gcatcatcag tggattgttg ttgtttttag cggctattga tgtagcgata gatattaaac
       61 accggcaccg taacaaagtt tctttaactt ttgatgaagt gaaagcactt ttactggtta
      121 ttacagtggt actactgttt gtattactgt tagatattct cggctttgtg atttgctcat
      181 ttttgttgct ttttatttta atgaaaatac gaggtgtcag acttttgcat tccgttatcg
      241 tttcgatatc ggcatctctt attatctggt tcatttttgc cagagtgttg ttggtcgcct
      301 ttcctgaagg aatcttgttt taaggatgca atatggatat tttgattcag gcactaacgt
      361 atattaattt gaatgccatt ctggccatta ttgctgctac ggcctttggg ttatttgtgg
      421 gggccatccc tggattgacc gcaacgatgg cggttgcact aatggtgcct tttaccttct
      481 ttcttgatcc tattcccgcc ctggcattaa tgattgccgt tggtgcgtca tccatttatg
      541 ccggagatat ccccggcgca cttttgcgta taccagggac acctgcttcg gcggcttatg
      601 ttgctgattc aaatgaactg gtcaagcaag ggaaggtgaa tcgcgtattg ggacttgggc
      661 tggtgagttc ggtcattggt ggcgtgattg gatcacttat tttgatgttg gctgcgcctg
      721 tactggcgaa atttgccctt aattttagtt cttatgaata cacatggctt tctcttttag
      781 gcttaacttg cgcgactctg gttgtgggta atcaaccact gaaaggcatg gtgaccttat
      841 gtttgggatt attactggcg tgtatcggtt atgatcaaat atccggagtt ccacgtttta
      901 cgtttggtca aatcagcctg ttacaagggg tgaactttat cccggcgatg attggtctgt
      961 tcgcgatatc cggcgcaatt gaatattatc gtgacaaaat tagccaggta agtggtgttg
     1021 tgccacaaac gcaaacgggg attaatttat tcaagggaat ggggcaggtg atttggtcac
     1081 gaaaattaaa tcttggccga agtagtatta tcggtacgct tatcggagca ttacccggcg
     1141 ctggagcgga tattgctgcg tggataagtt acgccatttc aaaaaaattc tctcgccagc
     1201 aggagttata tgggcatgga tctgaggagg caattgttga ttcctcgagc agtaacaacg
     1261 ccagtctggc ggggagttgg atcccttcat tagttttcag tattccgggc gattctgccg
     1321 ctgcaattat catcggcgtg ctctatatga aggatatgaa tccagggccg acattatttc
     1381 tgttccaggc agataaactt tatgccgtat ttatcttgtt tctgatcgcc aatattgcat
     1441 tactaccgtt agccacaatt gcggtcagtt tcatcaaacg catcatctgg attgataaag
     1501 cgattctgta cccgattatt ctgattttca gtattgtcgg tgcatttgct atcgataact
     1561 cgggtgcttc tgtcgttgtc atgttggtca tgggggttct tggatactgg ttacaacgta
     1621 aggaataccc ggtttcgccc attattctgg ggatgattct tgggccaatg ttggaaaaaa
     1681 acttattatc ttcaatgatt aagtctaatg gcgaatggct ggcgtttgtt gagcgcccgg
     1741 tatctatggc actggctgtc tgtttctttt tggttgtcgc gttgcaagga cgcaatatct
     1801 accgttcatt tagccgttaa tccgttgttt tcagaccatc caagttgtgg atggtctgcc
     1861 ttcccctaag taaaatcctg cgtgactgct aaatttatac tctaaatgat tcgcgttgca
     1921 ggaatatatt catacaaaga atagattgac ctgaatacca ccactttgca caatccacga
     1981 cgaattcatc aaccgggcac attatgcaag cattgctgga acactttatt acccaatcca
     2041 ccgtgtattc attgatggcg gtggtgttgg tggcctttct ggagtcgctg gcgctggtcg
     2101 gtttgattct gcccggtacg gtgctgatgg cggggctggg agcgttgatt ggcagcggtg
     2161 agttaagttt ctggcacgcc tggctggcag ggatcgtcgg ttgcctgctg ggcgactgga
     2221 tctccttctg gctgggctgg cgttttaaaa agccgttgca tcgctggtca tttctgaaga
     2281 agaacaaagc attactcgat aaaaccgaac atgcgctgca tcagcacagc atgttcacca
     2341 ttctggtcgg tcgttttgtc ggcccgacgc gtccgctggt gccaatggtg gcagggatgc
     2401 tggatctgcc ggtggcgaaa tttattacgc caaatattat cggctgcctg ttatggccac
     2461 cgttttactt cctgccgggg attctggcgg gtgcggcgat cgatattcct gccgggatgc
     2521 agagcggtga gtttaaatgg ttgctgctgg tgacggcggt attgttgtgg gttggtggct
     2581 ggctgtgctg gcggttatgg cgcagcggta aagcgactga ccgtttgagt cattatttgt
     2641 cccgcggtcg tttgttgtgg ctgacgccgt tgatttctgc catcggcgtg gtggcgctgg
     2701 tggtgttaat tcgccacccg ttgatgccgg tgtatatcga tattttgcgt aaagtggttg
     2761 gggtttagag gatagccttg tgcgggttgc ctgatgcgac gcttgctgcg tcttatcagg
     2821 cctacaaaac gcactacctg taggtcggat aaggcgttca cgccgcatcc gacagtgcat
     2881 attaaccttt aattcccaac agcgccgaag cactcgcctt accgctcaac aactcatcgg
     2941 tcttaccctg ccaggcgata cgcccgtcgg cgaccaccac cgagcgcgtg gcgatccgcg
     3001 ccgcatcttc cacactgtgc gacaccatca agagcgtcat tttttgctgt tggcagctcg
     3061 aactcaccag cgtcagcatc tcctgacgta gcgccggatc gagtgcggaa aacggttcat
     3121 cgagcaataa aatgggctgt tcgcgtacca gacaacgcgc taacgccacg cgctgtcgct
     3181 gaccgccgga aagctcgcca ggtaaccgcg ccattaaatt atcaatcccc atctggtggg
     3241 caatcgcgtg cattttcttc tgctgcgccg catttagttt caaccccgga tttagcccca
     3301 gcccgatgtt ctgcgcgacc gtcaggtggc tgaacaggtt gttctcctga aacagcattg
     3361 acaccggacg acgtgacggt ggcgttgtgg tgtgatctac gccgtcgaga gtcagcaaac
     3421 cgctggctgg cgtcagaaaa ccggcgatca aatttagcag ggtgctttta cccgcgccgc
     3481 ttggcccgag gatcgccacc tgctcgccgc gttccaccgt taagctaaaa cgcatcggca
     3541 aatggtggta aagccaggtg atatcagtca gttttaacat ttcgccccgg tattttttca
     3601 atcacggtaa acagcagaaa acagagcagc agcagaatta acgcggtgac cgcgccgtcc
     3661 tggctgcgat aggagccaat ttgctgatag agataaaacg gcagggtacg gaaatcatcg
     3721 ttaccgaaca atgccaccac gccaaaatca ccaatcgaca gcacacaagc aaaggccagc
     3781 gcctgcgcca gtggacgttt cagagcgcgc agctccacca cttttaagcg tgaccagcct
     3841 tcaatgccca gcgactgaca caacatgctg tagcgggcgg tgatatcgcg catcgggttt
     3901 tccagtactt tcagcgcata agggattgcc attaacgcat tggtgaaaat cacaatgccg
     3961 tcagcagatt gtggcaggcc gatagtgttg ttgagcagta aaaagaagcc cgtagccagt
     4021 acaatccccg gcatggcgag gatcaacatg ccgctcatct ccagcgcctg acccgccagc
     4081 attttctgcc gtgcccgaag ttcgcgactg ctccatagca gcatcatggt cagcactacg
     4141 cacaatacgc ctgccgccag cgcaatacgc aacgaggtcc acagcgcctg ccacagcacc
     4201 ggttgtgcca gcacttccgg caactggcga tttaccccat cgacgatcac cgccagtaac
     4261 ggtggcagca acagcagcag cgccagcaca attaacgccg tgtcgcaaat gcgactatgc
     4321 agacgatcgt ccgggtcgcg ccagccttgc agcagtgtgg taccgggcgc aatggcctta
     4381 ctcaatcgct gactcaacag caccagcccg aggcagcaca ccatctggat cagcgccagc
     4441 attgccgcgc gggcaggatc gtagtcgtaa ctcagcgcct gatagattgc cagctcgata
     4501 gtggtcgcct gcggaccgcc gcccagcgat agcacggtgg cgaagctggc gaaacagagc
     4561 ataaagataa gcgcagcaac cggcgggatt tgtcgccgta accacggcca ttcgacgaag
     4621 cggaagaagt gccagccgcg catcccaagt tgtgcggcaa gctgacgttg ttcgccggga
     4681 atgttttcca gtgcctggag taataaacgg ctagccatcg gcagattaaa aaacacatgg
     4741 gccagcaaaa tgccttgcag gccgtagggc gaaaaggtcc actccagacc aagtgattgc
     4801 cagagcgagg ccagccagcc ctggcgacca tagacgctaa gaatgccgaa aacggcgacc
     4861 agcaccggga ggatcaaggt cattgcgcac agacgcaaca gcacctgtcg cccaggaaag
     4921 cgcctgcgat agagcgcgcg ggcgaggaat attgcgggta cgacagagag cagcgccgag
     4981 agaaacgcct gccagaagga gaagcgcacc acatgccaca gatagctgtc ctgccagact
     5041 gccacccagt caccctgcgg cgcgttccac cacagggcga gaaacgccgc cagcgcaacc
     5101 gctaccacca gtgtggcggc gcttattcct ggaattaacc agccgggaat taacggctga
     5161 cggcgcgttg ccattcgcta atccatgcct gacgttgtgc cgccacttcg gcgggcgtga
     5221 actctagcgt ggtggctggt ttggtcaatt gttcaaatcc tgcaggcaga gtgacgtttg
     5281 ctaccggata catccagttg ccggttggga tcgcattctg aaaagccgga gaaaccataa
     5341 actggaggaa tttttgcgcc agctctggct gcttgctggc agcggtgcgg gcggcaactt
     5401 ccacctgcag atagtgacct tcgctgaagt tcgcggcggc gtagttatct ttcttctctt
     5461 cgagaatgtg ataagccgga gaggtggtgt aactcagtac cagatcgctt tcgcctttta
     5521 aaaacaggcc gtaggcttcg ctccagcctt tagtgaccgt gacagttttc ttcgccagtt
     5581 tctgccaggc ttgcggggcg ttatcgccat agactttttg catccatagc aacagaccca
     5641 gacctggtgt actggtacgc ggatcttcat aaatcacccg ccagttttga tcgctctcaa
     5701 ccagttcttt caggctttgc ggcggatttt tcagtttgtt cttgtcatag acgaaggcga
     5761 aatagccata gtcaaacggc acaaaggtgt catttttcca gccgccggga acgtttacag
     5821 cctctgtcgc gacgccgctt ttggcaaaca acccggtttt gctggcggcg tccagcaggt
     5881 tgttatccag ccccagcacc acatcggctt tgctgttttt gccttccatc cgtagacggt
     5941 tgagcagcga aacgccatct tccagcgcca ccagtttcag ttcgcaatta cagtctgctt
     6001 caaaggcttt tttgactacc ggaccaggtc cccagtcagc ggcaaaggaa tcgtaggtat
     6061 aaacagtcag aacaggttta gcgaaaacag gcgctgtgca cagcaacagc aggggaagac
     6121 atttttttaa cactttgcac ctcacaaaag agtggcaaag gacttgagaa ggagcctcaa
     6181 atcccttcgc cggcgttatc cggatcaggt tcgacgggta ttttctcagc gcacgcggct
     6241 gcgtggcacc ccgttgagaa cggcgttagt gtagtgattt tgttatcaac cagcaatcat
     6301 ggatccggtg gtgcaaacca cgctgattta aaatcgaacc agccgagggt gttcatgcgc
     6361 aggccgcgca tactgcgttg cccctgaatg atcagccagt ggtggataag tggcaccatt
     6421 gctttgctgg cgaccagttg ctggcaccag ttcgccaggt tcatttcgcc attgcgccag
     6481 cgggcggcgt cggcttgcca gtcgatcgga atacagtgtt ggagtagcgg cacctcgcac
     6541 aagtgcgcga acagcgaaaa atccagcggc agcgtaaagt tggcgctgtt aagccagata
     6601 tcgctctcga tctctccttc atgccactga tcgtagctga tctctttgat ttccagcgtg
     6661 acctggtgac tcgccagaat ctgctgcatg atcccggcaa tcactcgatg ttcaatatga
     6721 tcctgataaa aggtcagggt gagatgttcc agcccggcgg gtttttcgca atgtgtcggg
     6781 cgggcatggt gccagcgggg gagcagtcca taagccggga accagagttg ctggtattgt
     6841 tcctcggcga aatagaccag attcgtcgga gaaagcacat agcttaccca gtccctgact
     6901 tgctgattcg ccccgcggtg ggtgcggttg tcgaacagta aataatagca accttcctcc
     6961 aggcggcttt caatctcttg ttcctcgccc tgcggccctt tcagcatcag ccctccggct
     7021 ggctcgtcgg caatttccgt tagaacccag acgttaactt cgtcgattaa tgcccggtaa
     7081 ccgaagaagt catcgaatgc ctgaattttc agttgattgg tgctgttgcg gatcaccgca
     7141 tacggaccgg tgccgatggg atggctggca aagttactga gggtttccca ttcgcgcggc
     7201 aggatcattg ccggaacttg ccccagcagt aacggcaacc agcggtccgg ttgcgtgaga
     7261 tggatatcca gcgtccaggg cgtcggcgac acaatgtcag caatatgcga atagagcgga
     7321 agcgtattga ttcgttttaa agaggcgatc acatcgtcca tttccagttc acgaccatgg
     7381 tgaaaatgga ctcctggacg caaaaagaaa cgccagtgaa gcggtgatat ttgctgccag
     7441 tggtgtgcga tgtctgcttc cagttcccca ttttcctcat ttatgcgcgt tagcgaactg
     7501 aagatttgcc gggcgatatg ggtttcagaa cggcgcaatg cgctgccagg tagcagatta
     7561 cgcaacggac gatagtagag cacgcgcagg atgtgccgcc cctggcggaa gctgcggccc
     7621 agatgagaaa ccagcatttg ccgcacagtc gctttgtcgc caaccaactg cactagttgg
     7681 tcgatacgat cctgctccag caggtcttcc gctcgctgtt gctgaagcgc cagcccggta
     7741 tagaggaatg tcagacgcga gcgtttaccg cgcccgactt ccgcttccca cgtcagccag
     7801 ccgcgatcct gcatggtgtt gagcagggtg cgcatatgac gacgcgagca actcagtaac
     7861 gctgccagtt cgttgagcgt ggtctcctga gatttaccct cgcagcattg ccacaggcgg
     7921 atgaactgtt gttgcagacg agcagatggc ataaaagggg aactcctgcg caaaagacag
     7981 caattttatt ttccctatat taagtcaata attcctaacg atgaagcaag gaggtgcccc
     8041 atgcgtcagt tttatcagca ctattttacc gcgacagcga agttgtgctg gttgcgttgg
     8101 ttaagcgtcc cacaacgatt aaccatgctt gaagaactga tgcaatggga ggggagtcat
     8161 tctgactact gatttgcgga catcatgtgt gactgagtat tggtgttaat cgccacgcca
     8221 gcagtgatta tctgctggct tttttctttt ggcgattgag tgccggacgg tgtacaaact
     8281 aacgcataaa cgccggttgc ttctcttcat aagacgcaat agcatcgtcg tgctgcagag
     8341 tcaggccgat gctgtccaga ccgttcatca tgcagtggcg gcggaaggca tcgatggtaa
     8401 agcgataagt tttctctccc gctttcacct cttgcgcttc caggtctaca tcaaaatgaa
     8461 tccccggatt ggctttcacc agcgcaaaca gctcatccac ttctgcatcg cttaatttca
     8521 ccggcagcag ctggttgtta aagctattgc cgtagaagat gtcagcaaaa ctcggcgcaa
     8581 tcaccacctt aaaaccgtaa tcggtcaatg cccacggcgc gtgctcacgc gaggaaccgc
     8641 agccgaagtt ttctcgtgcc agcaaaatgg aagcgccctg atactgcggg aagttcagca
     8701 cgaagtccgg gtttggctgt tggccttttt catccagaaa acgccagtcg ttaaacagat
     8761 gcgcgccaaa acccgtacgg gtcactttct gcaaaaactg tttcgggatg attgcatcgg
     8821 tatcgacatt ggcggcatcc aacggaacca ccaggcctgt gtgtttgata aatttctctg
     8881 ccatggtgtg ctccttattt aatgttacga atgtcggcga aatgtccggt cacagccgcg
     8941 gcggcagcca ttgccgggct gaccagatgc gtgcgcccgc cgcgcccctg gcggccttca
     9001 aagttacggt tgctggtgga ggcgcaacgt tcgcccggat tcagacggtc gttattcatc
     9061 gccagacaca ttgagcaacc tggcaagcgc cattcaaaac cagcttcgat aaaaattttg
     9121 tccagacctt ccgcttccgc ctgggcttta accggaccgg agccgggaac caccagcgct
     9181 tgtacgcctg gcgcgacttt ccggcctttg gcgatttccg ctgccgcgcg taaatcttca
     9241 atacgcgagt tagtacagga accaataaac actttgtcga tagccacttc tgtcagcgga
     9301 atacccggtt tcagccccat ataggccagc gctttttctg ccgacgcgcg ttcaaccgga
     9361 tcggcaaacg aagccggatc gggaatattg tcgttcacag aaatcacctg gcctggattg
     9421 gtgccccagg tgacctgcgg cgaaatctct tctgcttgca gagtgacaac ggtatcgaaa
     9481 gttgctcctt cgtcggtttg cagggttttc cagtaggcaa cggcgtcgtc gaaatcttta
     9541 cctttcggcg catgcagacg gcctttaaca tagttaaagg tggtttcgtc cggcgcaacc
     9601 agaccggctt ttgcgcccat ttcgattgcc atattgcaca gggtcatacg accttccatg
     9661 cttaaatcac ggattgcttc gccgcaaaat tccaccacat gcccggtacc gcctgcgctg
     9721 ccggttttac caataattgc cagcacgata tcttttgcgg taatgcccgg cgcggctttg
     9781 ccctggactt caattttcat ggtcttcgcc cggccctgtt tcagggtttg cgttgccagt
     9841 acgtgttcaa cttcggaagt gccgatacca aaggccagtg cgccaaacgc gccgtgggtg
     9901 gcggtatgtg agtcgccgca gacaatggtc atccccggca aggtcacacc ctgttccggc
     9961 cccattacgt ggacgatccc ctgatacggg tgattcaggt catacagctc gacgccaaat
    10021 tctttgcagt ttttgatcag ctcctgcatc tggatgcgcg ccatttcacc gcaggcatta
    10081 atgtctttcg tctgggtaga gacgttgtga tccatggtgg cgaaggtttt gcccggctga
    10141 cgtaccgggc gaccgtgggc gcgcagacca tcgaacgcct gcggtgaggt cacttcatgc
    10201 accaggtggc ggtcgatata taacagcggg gtttcgtttt cggcttcgta cactacgtga
    10261 gcgtcgaata atttttcgta taacgtctta gccatgatta caccccttct gccacatagc
    10321 gggcaatgat atcgcccatt tcatcggtac taacggcggc agcgccacgg gctaaatccc
    10381 cggtgcgaat gccttcttct aatgcgcggt taatggcgcg ttcaatggcg gaagccgcat
    10441 catcggcatc caggctgtaa cgcagcagca gcgccagcga cagaatttgt gcaatcgggt
    10501 tggcgatatt tttacctgcg atatcgggtg ccgagccgcc cgccggttca tacagaccaa
    10561 aaccttgctc gttcaggctg gcggaaggca acatccccat tgagccagtg atcattgcgc
    10621 actcatcaga cagaatgtcg ccaaacaggt tggagcacag caagacgtca aactgtgatg
    10681 gatctttaat cagctgcatg gtggcgttgt cgatgtacat atgcgccagt tcgacgtccg
    10741 ggtattccgt ggcgatctcg ttaacgatct cccgccataa aatagaggat tgcagcacgt
    10801 tggctttgtc gattgaggtg actttgtggc gacgcttgcg ggcagattca aatgcgatgc
    10861 gggcaatacg ctcgatctca aaacggtggt acacctcggt atcaaacgct ttttcatatt
    10921 gtccgctacc ttcgcggcct tttggctgac cgaaatagat gccgccggtc agttcgcgca
    10981 cacacaggat gtcaaagccg tttgcggcaa tgtcagcacg cagcggacag aatgcttcca
    11041 gcccctgata cagttttgcc ggacgcagat tgctgaataa tttgaagtgc ttacgcaaag
    11101 gcagcagtgc gccgcgttct ggttgctggt ctggcggcaa atgttcccac ttcggaccac
    11161 ctaccgagcc aaacagcacg gcatcggctt gctcacaacc ttcaaccgtc gcaggcggca
    11221 gcggctgccc gtgattatca atggctgcgc cgcctacatc gtaatggctg gtggtgatac
    11281 gcatcgcaaa tcggttgcgc acggcatcca gcactttcag cgcctgggtc atcacttccg
    11341 gaccaatacc gtcccccggc aatacggcaa tatggtaatt cttcgacatc acacggtttc
    11401 cttgttgttt tcgttgtgtt gagctttgcg ttgcaactct ttttcgactt ctgcggcacg
    11461 ccagatattg ttcagtacgt gcaccatggc tttggcggag gactcgacaa tatcggtggc
    11521 caggccgacg ccgtggaagc ggcgaccgtt gtagttggcg acaatatcca cctgacccag
    11581 cgcatcttta ccgtgacctt tggcggtcag gctgtatttc accagttcga cgttatagtc
    11641 agtgatgcgg tttatcgcct ggtagacggc atcaaccggg ccgttgccgt tagcggcttc
    11701 tgctttaact tcttcgccac aggccagttt gacggcggca gtggcaatat cgttagaacc
    11761 tgactgcacg ctgaagtaat ccagacggaa atgctccggc tcttcttgct gcttatcgat
    11821 gaaggccagc gcctccagat cgtaatcaaa cacctggcct tttttgtccg ccagcttcag
    11881 gaaagcgtcg tacaggttgt ctaaattata ttcactttct ttatatccca tctcatccat
    11941 gcgatgtttc accgccgcac gccccgaacg agaggtcaga ttcagctgga tttggttcag
    12001 accaatagat tctggtgtca tgatttcgta gttttcgcgg tttttcagta caccatcctg
    12061 gtggataccg gaggagtgtg cgaatgcgcc gctgccaaca atggctttgt ttgccgggat
    12121 cggcatatta caaatctggc taactaactg gctggtgcgc catatctcct ggtgattgat
    12181 ggcggtgtgg acgttgagaa tatccttacg aactttgatc gccatgatga cttcttcaag
    12241 ggaacagtta ccggcacgct cgccgatccc gttcattgcg ccttctacct gccgtgctcc
    12301 ggcatgtacc gccgccagtg agttgccgac cgccaggccc aaatcgtcgt gggtatgtac
    12361 ggaaataatg gctttgtcga tgttgggtac gcgttcatac aggccgctga tgattccggc
    12421 gaactcaaat ggcatggtgt agcccacggt gtccggaatg ttgatggtgg tggcaccggc
    12481 attaatcgcc gcttcgacca ctcgcgccag atcggcaatt ggggtgcgtc cggcatcttc
    12541 gcaagaaaat tcaacatcat cggtgtaatt acgggcgcgt ttcaccatat agatagcgcg
    12601 ttcgatcact tcgtccagcg tgctgcgcag cttggtggcg atgtgcattg gcgaagtggc
    12661 aataaaggta tgaatacgga aggcttcggc gactttcagg gattcggctg ccacgtcgat
    12721 atctttctcc acgcagcgag ctaacgcgca tacacggctg tttttaacct ggcgagcgat
    12781 ggtctgcact gattcaaaat cacccggcga agagacgggg aaaccgactt ccatcacgtc
    12841 aacacccata cgctcaaggg ccagcgcaat ttgcagtttt tctttcacac tcaagcttgc
    12901 ctgtaacgcc tgttcaccgt cgcgcaatgt ggtatcgaaa ataatgactt gctggctcat
    12961 ggtttgggtc cttgtctctt tttgagcgcc tcgcttcggg cataaaaaaa cccgcgcaat
    13021 ggcgcgggtt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
    13081 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
    13141 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
    13201 accctgaagc aataaaaacg tcacgccatt gtgagttcgg catccgtaaa attagctaat
    13261 tgtgctgcgg tggttaaaac aagcgatatt aatttctgct taaccgccga tgcttttcat
    13321 cggttgacat atttcagaat aattttttgc atctaatcaa cgagggaaag ggggataaaa
    13381 tgcacgcgtt acaaaaccta tcctgatgat ttgtattgaa ttatatgttt tgcgaatttt
    13441 ttgatattca tttggtgaat attattga
//
LOCUS       DAJOTB010000127        13352 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJOTB010000127 DAJOTB010000000
VERSION     DAJOTB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.127"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            103..1188
                     /gene="mltB"
                     /locus_tag="OHN28_003690"
     CDS             103..1188
                     /gene="mltB"
                     /locus_tag="OHN28_003690"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCQ2299008.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            complement(1227..1466)
                     /locus_tag="OHN28_003691"
     CDS             complement(1227..1466)
                     /locus_tag="OHN28_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299009.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            1648..2145
                     /gene="pncC"
                     /locus_tag="OHN28_003692"
     CDS             1648..2145
                     /gene="pncC"
                     /locus_tag="OHN28_003692"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCQ2299010.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            2225..3286
                     /gene="recA"
                     /locus_tag="OHN28_003693"
     CDS             2225..3286
                     /gene="recA"
                     /locus_tag="OHN28_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCQ2299011.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            3355..3855
                     /gene="recX"
                     /locus_tag="OHN28_003694"
     CDS             3355..3855
                     /gene="recX"
                     /locus_tag="OHN28_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCQ2299012.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            3984..6614
                     /gene="alaS"
                     /locus_tag="OHN28_003695"
     CDS             3984..6614
                     /gene="alaS"
                     /locus_tag="OHN28_003695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCQ2299013.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            6849..7034
                     /gene="csrA"
                     /locus_tag="OHN28_003696"
     CDS             6849..7034
                     /gene="csrA"
                     /locus_tag="OHN28_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCQ2299014.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            7350..7442
                     /locus_tag="OHN28_003697"
     tRNA            7350..7442
                     /locus_tag="OHN28_003697"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7384..7386,aa:Ser,seq:gct)
     gene            7446..7522
                     /locus_tag="OHN28_003698"
     tRNA            7446..7522
                     /locus_tag="OHN28_003698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7480..7482,aa:Arg,seq:acg)
     gene            7587..7663
                     /locus_tag="OHN28_003699"
     tRNA            7587..7663
                     /locus_tag="OHN28_003699"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7621..7623,aa:Arg,seq:acg)
     gene            7726..7802
                     /locus_tag="OHN28_003700"
     tRNA            7726..7802
                     /locus_tag="OHN28_003700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7760..7762,aa:Arg,seq:acg)
     gene            7866..7942
                     /locus_tag="OHN28_003701"
     tRNA            7866..7942
                     /locus_tag="OHN28_003701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7900..7902,aa:Arg,seq:acg)
     gene            8223..8789
                     /gene="yqaB"
                     /locus_tag="OHN28_003702"
     CDS             8223..8789
                     /gene="yqaB"
                     /locus_tag="OHN28_003702"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCQ2299015.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            8786..9214
                     /locus_tag="OHN28_003703"
     CDS             8786..9214
                     /locus_tag="OHN28_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2299016.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            9287..10843
                     /gene="gshA"
                     /locus_tag="OHN28_003704"
     CDS             9287..10843
                     /gene="gshA"
                     /locus_tag="OHN28_003704"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2299017.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            10993..11508
                     /gene="luxS"
                     /locus_tag="OHN28_003705"
     CDS             10993..11508
                     /gene="luxS"
                     /locus_tag="OHN28_003705"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCQ2299018.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(11559..12671)
                     /locus_tag="OHN28_003706"
     CDS             complement(11559..12671)
                     /locus_tag="OHN28_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2299019.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(12668..>13352)
                     /locus_tag="OHN28_003707"
     CDS             complement(12668..>13352)
                     /locus_tag="OHN28_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HCQ2299020.1"
                     /translation="RTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEKLDGENNCL
                     NRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIEYRALEQDF
                     YLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLALTNARGTF
                     DPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKRHWQRARMA
                     HEFVYGEQS"
BASE COUNT         3239 a         3223 c         3662 g         3228 t
ORIGIN      
        1 agttatgtaa agtccgtatc gggcagtgac taccgcttcc ttgtgcgggg cgggatgctt
       61 taccatactt gcccctggtt gaatctgtta aatggacccc tcatgttcaa gcgtcgttat
      121 gtaacattgc ttcccctttt tgtgttgctt gccgcctgta gcagcaagcc aaaacctact
      181 gagactgaaa cgaccaccgg aacgccgtct ggcggcttcc tgcttgagcc gcagcacaat
      241 gtgatgcaga tgggcggtga cttcgccaat aacccgaatg cccagcagtt catcgacaaa
      301 atggtgaaca aacacggttt cgatcgtcag cagttgcagg aaattctctc ccaggcgaaa
      361 cgtctggatt cggtactgcg gctgatggat aaccaggcac caaccacatc ggtgaaaccg
      421 ccatcaggtc cgaacggcgc atggctgcgt tatcgcaaaa aatttattac gccggacaac
      481 gtgcagaacg gtgtggtttt ctggaatcag tatgaagatg cgttgaatcg cgcgtggcag
      541 gtgtatggtg taccgccgga aattatcgtc gggattatcg gcgttgaaac ccgctggggg
      601 cgcgtgatgg ggaaaactcg cattctcgat gcgctggcaa cgctgtcatt taattaccct
      661 cgccgcgcgg aatatttttc aggtgagctg gaaaccttcc tgctgatggc gcgcgacgag
      721 caggacgatc cgctcaatct gaaaggttcc tttgcaggtg cgatgggtta cggtcagttt
      781 atgccatcgt cttacaaaca atatgcggtg gatttcagcg gtgacgggca tatcaacctg
      841 tgggatccgg ttgatgccat cggtagtgta gcgaactatt tcaaagcgca cggctgggtg
      901 aaaggcgatc aggtggcggt gatggcaaat ggtcaggcgc caggcttacc gaatggcttc
      961 aaaaccaggt acagcatttc acaacttgcc gcagctggac taacgccgca gcagtcgctg
     1021 gggaatcatc agcaggccag cttgctgcgt ctggatgttg gcactggtta ccagtactgg
     1081 tatggtctgc cgaacttcta cactattacc cgttataacc acagcactca ttacgcaatg
     1141 gcggtctggc agttaggaca ggccgtggcg ctggcgcgcg tgcagtaact atttgcaaga
     1201 gggaggttaa acttccctct gttatcttac tgtttattcg aatggcgtaa ttgcttgatt
     1261 tttttcgaca gcgatatcgc atatcccagg cagacaacgc ctaacgcagc ctgaccgaag
     1321 tcaaaaggtt caagatagcc agaagataaa actttacata tcacggaaat cagtgcgctg
     1381 atgccgccaa tgatgaccca catacgcatt tgtgtggttt gtttgagcac gttttgtggg
     1441 gtgttatccg ttttctttat tgccattgct attctctccc tgtgaataca tatcaatgtg
     1501 aacgcggcat ggtagcgctt tttctctacc gactggccgc aacagtcttt tttcggaacg
     1561 cgactcgtaa aatgaatcgc tcgtccccat cttttgcggg aaagtgctat cttgtccggc
     1621 ataaattttt gactgacaga ggttgtgatg actgaaagtg aactgatgca gttaagcgaa
     1681 caggttgggc tggcgctgaa agcccgtggc gcaaccgtaa caactgctga gtcttgtacc
     1741 ggcggttggg tagcgaaagt gattaccgat attgccggca gttccgcctg gtttgaacgc
     1801 ggatttgtca cctacagtaa cgaagcaaaa gcgcaaatga tcggtgtacg cgaagagacg
     1861 ctggcgcagc atggcgcggt gagcgaacct gtcgtggtgg aaatggcgat aggcgcactg
     1921 aaagcggctc gtgctgatta cgccgtgtct attagtggta tcgccgggcc ggatggcggc
     1981 agtgaagaga agcctgtcgg caccgtctgg tttgcttttg ccactgcccg tggtgaaggc
     2041 attacccggc gggaatgctt cagcggtgac cgtgatgcgg tgcgtcgtca ggctactgcg
     2101 tatgcattgc agaccttgtg gcaacaattt ctacaaaata cttgatactg tatgagcata
     2161 cagtataatt gcttcaacag aacatattga ctatccggta ttacccggca tgacaggagt
     2221 aaaaatggct atcgacgaaa acaaacagaa agcgttggcg gcagcactgg gccagattga
     2281 gaaacaattt ggtaaaggct ccatcatgcg cctgggtgaa gaccgttcca tggatgtgga
     2341 aaccatctct accggttcgc tttcactgga tatcgcgctt ggggcaggtg gtctgccgat
     2401 gggccgtatc gtcgaaatct acggaccaga atcttccggt aaaaccacgc tgacgttgca
     2461 ggtgatcgcc gcagcgcagc gcgaaggtaa aacctgtgcg tttatcgatg ctgaacacgc
     2521 gctggaccca atctacgcac gtaaactggg cgtcgatatc gacaacctgc tgtgctccca
     2581 gccggacact ggcgagcagg cactggaaat ctgtgacgcc ctggcacgtt ctggtgcagt
     2641 agacgttatc gtcgttgact ccgtggcggc actgacgccg aaagcggaaa tcgaaggtga
     2701 aatcggcgac tctcacatgg gccttgcggc acgtatgatg agccaggcga tgcgtaagct
     2761 ggcgggtaac ctgaagcagt ccaacacgct gctgatcttc atcaaccaga tccgtatgaa
     2821 aattggtgtg atgttcggta acccggaaac cactaccggt ggtaacgcgc tgaaattcta
     2881 cgcctctgtt cgtctcgaca tccgtcgtat cggcgcggtg aaagagggcg aaaacgtggt
     2941 gggtagcgaa acccgcgtga aagtggtgaa gaacaaaatc gctgcgccgt ttaaacaggc
     3001 tgaattccag atcctctacg gcgaaggtat caacttctac ggcgagctgg ttgacctggg
     3061 cgtgaaagag aagcttatcg agaaagcagg cgcgtggtac agctacaaag gtgagaagat
     3121 cggtcagggt aaagcgaatg cgactgcctg gctgaaagat aacccagaaa ccgcgaaaga
     3181 gatcgagaag aaagtacgtg agttgctgct gagcaacccg aactcaacgc cggatttctc
     3241 tgtagatgac agcgaaggcg tagcagaaac taacgaagat ttttaatcgt cttgtttgat
     3301 acacaagggt cgcatctgcg gcccttttgc ttttttaagt tgtaaggata tgccatgaca
     3361 gaatcaacat cccgtcgccc ggcatatgct cgcctgttgg atcgtgcggt acgcattctg
     3421 gcggtgcgcg atcacagtga gcaagaactg cgacgtaaac tcgcggcacc gattatgggc
     3481 aaaaatggcc cggaagagat tgatgccacg gcagaagatt acgagcgcgt tattgcctgg
     3541 tgccatgaac atggctatct tgatgacagc cgatttgttg cgcgctttat cgccagccgt
     3601 agccgcaaag gttatggacc tgcgcgtatt tgtcaggaac tgaatcagaa aggtatttcc
     3661 cgcgaagcga cagaaaaagc gatgcgtgaa tgtgacatcg actggtgcgc actggcgcgc
     3721 gatcaggcga cgcgaaaata tggcgaacct ttgccaactg tcttttcaga aaaagttaag
     3781 atccagcgtt ttctgctcta tcgtggctat ctgatggaag atatccagga tatttggcga
     3841 aattttgccg actgaacgca tacgggattt tacttcccag tcaagaaaac ttatcttatt
     3901 cccacttttc agttaccagc cgggcggtta acgacacgca acggctggta gcgatatttc
     3961 gttagcttga tttcaggata attatgagca agagcaccgc tgagatccgt caggcgtttc
     4021 tcgacttttt ccatagtaag ggacatcagg tagttgccag cagctccctg gtaccccata
     4081 acgacccaac tctgttgttt accaacgccg ggatgaacca gttcaaggat gtgttccttg
     4141 ggctcgacaa gcgtaattat tcccgcgcta ccacttccca acgctgcgtg cgtgcgggtg
     4201 gtaaacacaa cgatctggaa aacgtcggtt acaccgcgcg tcaccatacc ttcttcgaaa
     4261 tgctgggcaa cttcagcttc ggcgactatt tcaaacacga tgccattcag tttgcatggg
     4321 aactgctgac cagcgaaaaa tggtttgccc tgccgaaaga gcgtctgtgg gttaccgtct
     4381 atgaaagcga cgacgaagcc tacgaaatct gggaaaaaga agtagggatc ccgcgcgaac
     4441 gtattattcg catcggcgat aacaaaggtg cgccatacgc atctgacaac ttctggcaga
     4501 tgggtgacac tggtccgtgc ggcccgtgca ccgaaatctt ctacgatcac ggcgaccaca
     4561 tttggggcgg ccctccggga agtccggaag aagacggcga ccgctacatt gagatctgga
     4621 acatcgtctt catgcagttc aaccgccagg ccgatggcac gatggaaccg ctgccgaaac
     4681 cgtctgtaga taccggtatg ggtctggaac gtattgctgc ggtgctgcaa cacgttaact
     4741 ctaactatga catcgacctg ttccgcacgt tgatccaggc ggtagcgaaa gtcactggcg
     4801 cgaccgatct gagcaataaa tcgctgcgcg taatcgctga ccacattcgt tcttgtgcgt
     4861 tcctgatcgc ggatggcgta atgccgtcca atgaaaaccg tggttatgta ctgcgtcgta
     4921 tcattcgtcg cgcagtgcgt cacggtaata tgctcggcgc gaaagaaacc ttcttctaca
     4981 aactggttgg tccgctaatc gacgttatgg gctctgcggg tgaagacctg aaacgccagc
     5041 aggcgcaggt tgagcaggtg ctgaagactg aagaagagca gtttgcccgt actctggagc
     5101 gcggtctggc gctgctggat gaagagctgg caaaactttc tggtgatacg ctggatggtg
     5161 aaactgcttt ccgtctgtac gacacctacg gcttcccggt tgacctgacg gctgacgttt
     5221 gccgtgagcg caacatcaaa gttgacgaag ctggttttga cgcagcaatg gaagagcagc
     5281 gtcgtcgtgc gcgcgaagcc agcggctttg gtgcagatta caacgcaatg atccgtgttg
     5341 acagtgcatc tgaatttaaa ggctatgacc atctggaact gaacggcaaa gtgactgcgc
     5401 tgtttgttga tggtaaatcg gttgatgcca tcaatgcagg ccaggaagct gtggtcgtgc
     5461 tggatcaaac gccattctat gcggaatccg gcggtcaggt tggtgataaa ggcgaactga
     5521 aaggcgctaa cttctccttc gcggtggaag atacgcagaa atacggccag gcgattggtc
     5581 acatcggtaa acttgctgtg ggttctctga aagtgggcga cgctgtgcag gctgatgttg
     5641 atgaggctcg tcgcgcccgt attcgtctga atcactccgc aacgcacctg atgcacgctg
     5701 cgctgcgcca ggttctgggt actcatgtat cgcagaaagg ttcactggtt aacgacaaag
     5761 tgctgcgctt cgacttctca cacaacgaag cgatgaaacc agaagagatt cgtgcggtcg
     5821 aagacctggt gaacgcgcag attcgccgta acttgccgat cgaaaccaac atcatggatc
     5881 tcgaagcggc gaaagcgaaa ggtgcgatgg cgctgtttgg cgagaagtat gatgagcgcg
     5941 tacgcgtgct gagcatgggt gatttctcca ccgagttgtg tggcggtact cacgccagcc
     6001 gcactggtga tattggtctg ttccgcatca tctctgaatc gggtactgct gcaggcgttc
     6061 gtcgtatcga agcggtaacc ggagaaggcg ctatcgccac cgttcatgca gacagcgatc
     6121 gcttaagcga agtcgcgcat ctgctgaaag gcgatagcaa taatctggct gataaagtgc
     6181 gttcagtact ggaacgtacg cgtcagctgg aaaaagagtt acaacagctt aaagaacaag
     6241 ctgccgcaca ggagagcgca aatctttcca gtaaggcaat tgatgttaat ggtgttaagc
     6301 tgttggttag cgagcttagc ggtgttgagc cgaaaatgtt gcgtaccatg gttgacgatt
     6361 taaaaaatca gctggggtcg acaattatcg tgctggcaac ggtagccgaa ggtaaggttt
     6421 ctctgattgc aggcgtatct aaggacgtca cagatcgtgt gaaagcaggg gaactgattg
     6481 gtatggtcgc tcagcaggtg ggcggcaagg gtggtggacg tcctgacatg gcgcaagccg
     6541 gtggtacgga tgctgcggcc ttacctgcag cgttagccag tgtgaaaggc tgggtcagcg
     6601 cgaaattgca ataatataag cgtcaggcaa tgccgtggac tcgcttcacg gcattcgcat
     6661 taacgctatc gacaacgata aagtcaggtt gaagttgtgt atatcggcta aacttaggtt
     6721 taacagaatg taatgccatg actgcttaga tgtaatgtgt ttgtcattgc ttactttttg
     6781 gcgttatatg atggataatg ccgggataca gagagacccg actcttttaa tctttcaagg
     6841 agcaaagaat gctgattctg actcgtcgag ttggtgagac cctcatgatt ggggatgagg
     6901 tcaccgtgac agttttaggg gtaaagggca accaggtacg tattggcgta aatgccccga
     6961 aggaagtttc tgttcaccgt gaagagatct accagcgtat ccaggctgaa aaatcccagc
     7021 agtccagtta ctaatctttc cgcgtctcat ctttatcggt gagacgcacc ctcaagattt
     7081 ctctttcact ccattgtctt ttcgctttac tcccgttcat tcaacttaag tctccatttt
     7141 tttgcattac tgctatctgt cagacctccg tttttctgtt gataaaacac tctttttgac
     7201 gtttttacag actaattgag cgtgaagtgt gcaaacgata aaagtgtggg aaaaattgtt
     7261 tgacttataa gtctcagaaa gtaatatgtg cgccacgcag cgacgatgag cgataaacaa
     7321 gttcttcgaa gcactcgtaa gaggcgtgtg gtgaggtggc cgagaggctg aaggcgctcc
     7381 cctgctaagg gagtatgcgg tcaaaagctg catccggggt tcgaatcccc gcctcaccgc
     7441 catttgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc ggtcggaggt
     7501 tcgaatcctc ccggatgcac catctcttac ttgatatggc tttagtggcg gtatcaaaaa
     7561 atctgcagta aagtaagttt cccgatgcat ccgtagctca gctggataga gtactcggct
     7621 acgaaccgag cggtcggagg ttcgaatcct cccggatgca ccatctctta cttgatatgg
     7681 ctttagtggc ggtatcaata tcagcagtaa aataaatttc ccgatgcatc cgtagctcag
     7741 ctggatagag tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc ccggatgcac
     7801 catctcttac ttgatatggc tttagtagcg atatcaatat cagcagtaaa ataaattttc
     7861 ccgatgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc ggtcggaggt
     7921 tcgaatcctc ccggatgcac catattctcc gtaaccttca gcaatgaagg tatcgaagag
     7981 gtagcggtat taaccgcagg caccagggag gataacgttg cgccagcagc ggcccgaagg
     8041 gcgaacgcca gtgagtcatc ctcccggatg caccatatta tccaaaaaat ttccttcaaa
     8101 aatatctcat ttctaaataa ctactttatc catttcctga attttcttct ccttctccat
     8161 cagcgacaaa atcaagcaat tacgctaaag tagcgtcata tttttcgact tgcgagaaga
     8221 ccatgtacga gcgttatgca ggtttaattt ttgatatgga tggcaccatc ctggataccg
     8281 agcctacgca ccgtaaagcg tggcgcgaag tattagggca ctacggtctt cagtacgatg
     8341 ttcaggcgat gattgcgctt aatggctcgc ccacctggcg tattgctcag gcgattattg
     8401 agctgaatca ggccgatctc gacccgcatg cgttagcgcg tgaaaaaaca gaagcagtaa
     8461 gaagtatgct gctggatagt gtcgagccgc ttcctcttgt tgaagtggtg aaaagctggc
     8521 atggtcgtcg cccaatggct gtaggaacgg ggagtgaaag cgccatcgct gaggcattgc
     8581 tggcgcacct gggattacgc cgttattttg acgccgtcgt cgctgccgat cacgtcaaac
     8641 accataaacc cgcgccagac acatttttgt tgtgcgcgca gcgtatgggc gtgcaaccga
     8701 cgcagtgtgt ggtctttgaa gatgccgatt tcggtattca ggcggcccgt gcagcaggca
     8761 tggacgccgt ggatgttcgc ttgctgtgag tgaagcgtta tcgcttttct cgttgtttgc
     8821 cagtagtttt ctcagcgcta cactattacc cggcaactcc gaagtcgtgc tggtggcaat
     8881 gttgctttcc ggagtgagtc atccctgggt tttagtttta acagcaacaa tgggtaatag
     8941 ccttggaggg ttaactaacg ttatccttgg gcgtttcttt ccattgcgta aaacatcgcg
     9001 ctggcaagag aaagctaccg gctggctgaa acgctatggt gcagtcacgc tattattaag
     9061 ctggatgccc gtggttggcg atttactgtg tctgttagcg ggatggatgc gcatctcgtg
     9121 gggaccggta atcttttttt tgtgccttgg taaagcgtta cgctatgttg cagttgcagc
     9181 agctaccgtt cagggcatga tgtggtggca ctaattgtag gcctgcacat atggtcacca
     9241 ttacagttat gctaattaaa acgattttga caggcgggag gtcaatttga tcccggacgt
     9301 atcacaggcg ctggcctggc tggaaaaaca tcctcaggcg ttaaagggga tacagcgtgg
     9361 gctggagcgc gaaactttgc gtgttaatgc tgatggcaca ctggcaacaa caggtcatcc
     9421 tgaagcatta ggttctgcac tgacccataa atggattact accgattttg cggaagcatt
     9481 gctggaattt attacaccag tggatggtga tattgaacat atgctgacct ttatgcgcga
     9541 tctgcatcgc tatacggcgc gcaatatggg cgatgagcga atgtggccgt taagtatgcc
     9601 atgctacatc gcagaaggtc aggacatcga actggcacag tacggcactt ctaacaccgg
     9661 tcgctttaaa acgctgtacc gtgaagggct gaaaaatcgc tacggcgcgc tgatgcaaac
     9721 catttccggc gtgcactata atttctcttt gccaatggca ttctggcagg cgaagtgcgg
     9781 tgatatctcg ggcgctgatg ccaaagagaa aatttctgcg ggttatttcc gcgttatccg
     9841 taactactat cgcttcggtt gggtcattcc ttatctgttt ggtgcgtctc cagcgatttg
     9901 ttcttctttc ttgcaaggaa aaccaacgtc gctgccgttt gagaaaaccg agtgcggtat
     9961 gtattacctg ccgtatgcga cctctcttcg tttgagcgat ctcggctata ccaataaatc
    10021 gcaaagcaat cttggtatca ccttcaatga tctctacgag tacgtggcgg gccttaaaca
    10081 ggcaatcaaa acgccatcgg aagagtacgc gaagattggt attgagaaag acggtaagcg
    10141 gctgcaaatc aacagcaatg tgctgcagat tgaaaacgaa ctgtacgcgc cgattcgtcc
    10201 aaaacgcgtt acccgcagtg gtgagtcgcc ttctgatgcg ctgttacgtg gcggcattga
    10261 atatattgaa gtccgctcgc tggacatcaa cccgttctcg ccgattggtg tagatgaaca
    10321 acaggtgcga ttcctcgacc tgtttatggt ctggtgtgcg ctggctgacg caccggaaat
    10381 gagcagtagc gaacttgcct gtacacgcgt taactggaac cgggtgatcc tcgaaggtcg
    10441 caaaccgggc ctgacgctgg gtatcggctg cgaaaccgcg cagttcccgt taccgcaggt
    10501 gggtaaagat ctgttccgcg atctgaaacg cgtcgcgcaa acgctggata gcattaacgg
    10561 cggcgaagcg tatcagaaag tgtgtgatga actggtcgcc tgcttcgata atcccgatct
    10621 gactttctct gcccgtatct taaggtctat gattgatact ggtattggcg gaacaggcaa
    10681 agcgtttgct gaagcgtacc gtaatctgct gcgtgaagag ccgctggaaa ttctgcgcga
    10741 agaggatttt gtcgccgagc gcgaggcgtc tgaacgccgt cagcaggaaa tggaagccgc
    10801 tgataccgaa ccgtttgcgg tgtggctgga aaagcacgcc tgacagaaaa gaaaaaggcc
    10861 actcgtgagt ggccaaaatt tcatctctga attcagggat gatgataaca aatgcgcgtc
    10921 tttcatatac tcagactcgc ctgggaacaa agagttcaga aaattttaaa aaaaattacc
    10981 ggaggtggct aaatgccgtt gttagatagc ttcacagtcg atcatacccg gatggaagcg
    11041 cctgcagttc gggtggcgaa aacaatgaac accccgcatg gcgacgcaat caccgtgttc
    11101 gatctgcgct tctgcgtgcc gaacaaagaa gtgatgccag aaagagggat ccataccctg
    11161 gagcacctgt ttgctggttt tatgcgtaac catcttaacg gtaatggcgt agagattatc
    11221 gatatctcgc caatgggctg ccgcaccggt ttttatatga gtctgattgg tacgccagat
    11281 gagcagcgtg tcgctgatgc ctggaaagcg gcaatggaag acgtgctgaa agtgcaggat
    11341 cagaatcaga tcccggaact gaacgtctac cagtgtggca cttaccagat gcactcgttg
    11401 caggaagcgc aggatattgc gcgtaacatt ctggaacgtg acgtgcgcat caacagcaac
    11461 gaagaactgg cgctgccgaa agagaagttg caggaactgc acatttagtc agtttaagca
    11521 gaagaggccg ggctggtgaa aacaccgccc ggcttttgtt acctgtcagt catccgatat
    11581 tccacgctat gtgcttcatc aggttgagga acttccagcc ttgaggccat ccgcatcacg
    11641 accgcttcag gaactgcata ctcgcgtctg gcattttgct ggttccattg cgcccatggc
    11701 acttccagat aaacgatttt tactcgtgcg ccgtaagccg taaacagact gatgagctgg
    11761 ctgcgcagct ggcgggtaat gttcgttgca ttccagatga acggcttttt ctggcgtaaa
    11821 aaaacgcgcg cctcttcttt tgcctgttgc acaatacgcc cggtggcggt tttgtcgtcc
    11881 ggactggcat tgattcgccg ccgcatatca tcaaggctga taacgtccat tccctggcac
    11941 tgttcactga tatagcgatc tttgcccata cccggtaagc cacacagcaa aataacctca
    12001 aaaggttcag cctcccaggg aacaaaatca ggagaacttt gctcatgagt caggtagtgc
    12061 cagcgtgcgg aatcagaaac gaacgggcgc atttttcccc agcattgttg ctcatggcaa
    12121 aacagctcga acagatcgat gcgttccagc attgattgct gatccgggct ttggcgacca
    12181 agcagatcgg cacgagcaag caaagcgagc agacgcgtgt cgatgcgcat tgcagccgtg
    12241 agcaacaaac gctccggttc cggacgttcc agtaaccata gcggtaaccc atgcagacgc
    12301 accagtgcaa caatttgctc gcgcagcacg aacggcgttg ggatatcgcg ccataaaatt
    12361 tgtcgagccg tcagttcgcc ccgacgagca tgaccaggag actgaatacg tccgttttcc
    12421 tgcacggtag tgctgcgctt ttcaacatca tgcaataacg ccgccgccca taaaacttcc
    12481 tgctgttgag cggggagttg ctggaattct ggcagagtaa tgagggcgtt aagcaccatt
    12541 tcggtatgta cgccgacgtc gccttcacca tgatgctcag ggtcttgtgg cgtgtgatgc
    12601 atctcctcaa cccagctaaa acgctgacgt agcgccgacc agcgtttatc gtcggtaagt
    12661 tgccaaatca tgactgttct ccataaacga actcatgggc catccgcgcc cgttgccagt
    12721 ggcgtttcca gtgcacagtg gttttgacat gatttttgcg cacatacttg aagacgttat
    12781 gcgaaaagtc agcgacagaa aacgcatcat gatttcggct gacaatgcct tccagcgtac
    12841 agggttgaca ggtttgcgta tcccagggat cgaatgtgcc gcgcgcattg gtcagcgcca
    12901 gaaaatcacg ttgccagctt ttttcatcat tccccggctg cgggccggag atttcgggaa
    12961 cgcaggggaa atcaaacaga gcggcgtaaa actgcacctc ttcccagctc agccacatat
    13021 cctggcagcg aacggcaaac aggtaaaagt cctgttccag cgcgcggtat tcaatggaat
    13081 gaacggcgta gagattttca ccaaacaact ccaggttgcc gagatcgttt ttcagcagtt
    13141 gccagcgttg gcgaatttta taggtccatg ccgactgcgt aggcgtggca tgggagcggg
    13201 caaacacgcc gtagcggtta aggcagttat tttcgccatc gagtttttct gtgtgcacca
    13261 attgcgtgat ggtctgtaaa tcctgccagt aatcggtgtt aatgcggtca tcgctggtgg
    13321 tgcctggaga gaaggggtaa tgccaggttc tc
//
LOCUS       DAJOTB010000128        13348 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJOTB010000128 DAJOTB010000000
VERSION     DAJOTB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.128"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(275..1165)
                     /gene="rarD"
                     /locus_tag="OHN28_003708"
     CDS             complement(275..1165)
                     /gene="rarD"
                     /locus_tag="OHN28_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCQ2299021.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRISK"
     gene            complement(1217..1684)
                     /locus_tag="OHN28_003709"
     CDS             complement(1217..1684)
                     /locus_tag="OHN28_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCQ2299022.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1849..2718
                     /gene="pldA"
                     /locus_tag="OHN28_003710"
     CDS             1849..2718
                     /gene="pldA"
                     /locus_tag="OHN28_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCQ2299023.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSCSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2843..4672
                     /gene="recQ"
                     /locus_tag="OHN28_003711"
     CDS             2843..4672
                     /gene="recQ"
                     /locus_tag="OHN28_003711"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCQ2299024.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4737..5357
                     /gene="rhtC"
                     /locus_tag="OHN28_003712"
     CDS             4737..5357
                     /gene="rhtC"
                     /locus_tag="OHN28_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCQ2299025.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5395..6255)
                     /locus_tag="OHN28_003713"
     CDS             complement(5395..6255)
                     /locus_tag="OHN28_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2299026.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6414..7034)
                     /gene="rhtB"
                     /locus_tag="OHN28_003714"
     CDS             complement(6414..7034)
                     /gene="rhtB"
                     /locus_tag="OHN28_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCQ2299027.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7145..8167
                     /gene="pldB"
                     /locus_tag="OHN28_003715"
     CDS             7145..8167
                     /gene="pldB"
                     /locus_tag="OHN28_003715"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCQ2299028.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8175..8975
                     /gene="yigL"
                     /locus_tag="OHN28_003716"
     CDS             8175..8975
                     /gene="yigL"
                     /locus_tag="OHN28_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCQ2299029.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            9051..9950
                     /gene="bioP"
                     /locus_tag="OHN28_003717"
     CDS             9051..9950
                     /gene="bioP"
                     /locus_tag="OHN28_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCQ2299030.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9838..10791)
                     /gene="metR"
                     /locus_tag="OHN28_003718"
     CDS             complement(9838..10791)
                     /gene="metR"
                     /locus_tag="OHN28_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCQ2299031.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10909..13170
                     /gene="metE"
                     /locus_tag="OHN28_003719"
     CDS             10909..13170
                     /gene="metE"
                     /locus_tag="OHN28_003719"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCQ2299032.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         3221 a         3319 c         3532 g         3276 t
ORIGIN      
        1 attcatgttg ataacgagat aatgtaaaag aatggtagat gttataacaa agaaaccagc
       61 cccggcattg gaaaaaaaag aaataagtaa gatacaaatt agatttgtaa aaataaatga
      121 aaatatatag cggcgtctgg aaattctgcc tttagatgtt ggaataaata tacgcaacat
      181 taattttaca tccttgtgta agaatttcgt tagcatggca atattcccgc tcttatgcaa
      241 gagcaggcat attttttcag aatatattta ttttttattt ggaaattcta cgctgcgtat
      301 aaatcgcatc catcacaaaa attgccagcg ccacccaaat aaaggcgaaa gtcaccatct
      361 tatcggcacc cggtttttca ccataaaacg tcacagccag caggaacatc agcgtcggac
      421 caatgtactg gaaaaagcct aacgttgaga gacgcaggcg tgtcgcagct gcagtaaaac
      481 acaacagtgg cacggtggtg acaataccgg cggcgatcag cagtaaattc agcgacatcg
      541 ggttttgccc catatgactg gttgagctgt cggcaatagc aaacaggtaa atcgccgcca
      601 cgggcagcaa ccacatagtt tcgattaaca tgccggtttg cgcttcaaca gcaatcttct
      661 tgcgcaccag gccgtagaaa gcaaaactaa acgccagccc cagcgcgatg atcggcagtg
      721 aaccaaaagt ccacaactga accaacacgc cacaaatcgc cagaatcacc gccagccatt
      781 gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc accagtgggt
      841 taataaagta accaaggctc gcttccagca tatggtgatt gttcaccgcc cagataaaca
      901 gtagccagtt gccaccaatc agcacggcag agactgccag cataaaaatt ttctgtggcg
      961 tctgaatcag cgtttttaaa taggaccact ggcggcaaat gctcatcagc accaccataa
     1021 agaaaaacga ccagatcacg cgatgcgtca ggatctcatc ggcgggcacg tagtaaatca
     1081 gtttgaagta cgctggcgca ataccccaaa taaaataagc ggcaagagcg agtaatacgc
     1141 cctgccgcgt ttgttttgca tccatcggga atactcattt ttaattggta acagcagttt
     1201 acctgctttt atgtcttcaa cctaccatat aggtggcggt ggcactggca atataaagct
     1261 gttcttcatt gtgtaattca acgcgggcga cggcgacttt attgcctgca cgcaacaggc
     1321 tactggtagc agtaaaacgc tctcctctgc ctgggcgcag ataatcgacg cgaagatcaa
     1381 tggtccccat ccgcgatagc cgctggcgta gttcatcttc actgatggtt tcgtggcggg
     1441 ttaaggtact tcccacgcac accagacctg cggcgacatc cagcgccgac gcaatgaccc
     1501 cgccgtgtaa aatgctttgc gcccagttgc ccaccatcat cggctgattt ttaaaggcca
     1561 gctgtgcgaa ctctttttcg taacgctcca gttccatccc caatgcgcgg ttaaatggca
     1621 tgtgataaac aaacatctca cccactaatt tgagggcttg ttcagcggtc agtacggcag
     1681 acataacatc cttacacttc attagttaat gaaatgttga ttttatgctt atttattgtt
     1741 ggtttctact ttaggaaggg ataactaacg gctatggagt taagtatgta aaatagcccg
     1801 cagaaaaata ttcaccttat caataattcg ttacggagaa cacgagcgat gcggactctg
     1861 cagggctggt tgttgccggt gtttatgttg cctatggcag tatatgcaca agaggcaacg
     1921 gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa tatgctgcag
     1981 gagcatgaca atccgttcac cctttatcct tatgacacca actacatcat ctacacccaa
     2041 accagcgatc tgaataaaga agcgattgcc agttacgact gggcggaaaa cgcgcgtaag
     2101 gatgaagtaa agttccagtt gagcctggcg tttccgctgt ggcgtgggat tttggggcca
     2161 aactctgtac tgggcgcctc ctacacacag aaatcctggt ggcagctttc taatagcgat
     2221 gagtcatcac cgtttcgtga aactaactac gaaccgcaat tgttcctcgg ttttgccacg
     2281 gattacaact ttgctggctg gacgctgcgc gatgtggaaa tgggctacaa ccacgactca
     2341 aacgggcgtt cagacccgac ttcctgcagc tggaaccgcc tttatacccg cctgatggca
     2401 gaaaacggta actggttggt agaggtgaag ccgtggtatg tggtggggaa tacggacgat
     2461 aacccggaca tcaccaaata tatgggttac taccagctta aaatcggcta tcacctcggt
     2521 gatgcggtac tgagcgcgaa agggcagtac aactggaaca ccggttacgg cggcgcggag
     2581 ttaggcttaa gctacccgat caccaaacat gtgcgccttt atactcaggt ttacagcggt
     2641 tacggcgaat cgctcatcga ctataacttc aaccagaccc gcgttggtgt gggagttatg
     2701 ctaaacgacc tgttttgatg aatggttggc aaatctggaa tccagcatcc aggattaccc
     2761 tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt
     2821 atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc
     2881 taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagagat
     2941 tatcgacacc gtactttccg gtcgcgattg cctggtcgtc atgcccactg gtggcggaaa
     3001 atccctttgc tatcaaatcc ctgccttatt gctaaacggc cttaccgtgg ttgtctcacc
     3061 gctgatttcg ttgatgaaag atcaggtaga tcaactgcaa gccaacggcg tggcggcggc
     3121 gtgccttaac tcgacgcaaa cccgcgagca gcaacttgaa gtgatgacag gctgtcgcac
     3181 cgggcaaatc agcctgcttt atatcgcgcc ggaacgcctg atgctggata actttcttga
     3241 gcatctggcg cactggaatc cggtgttatt agccgttgat gaggcgcact gtatctccca
     3301 atggggtcac gatttccgcc cggaatacgc tgccctcggt cagctgcgtc agcgttttcc
     3361 gacgctgccg tttatggcgc tgactgccac agccgatgac accacgcgcc aggatatcgt
     3421 gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat
     3481 tcgctacatg ctgatggaga aattcaaacc gctcgatcag ttgatgcgtt atgtacagga
     3541 acaacgcggt aagtcgggga ttatttactg taatagtcgt gcgaaagtag aagacaccgc
     3601 tgcgcgcctg caaagcaagg gaattagcgc ggcggcctat catgccgggc tggaaaataa
     3661 tgttcgcgct gatgtgcagg aaaaattcca gcgcgatgac ctgcaaattg tggtggcgac
     3721 ggtggcgttc ggcatgggca tcaacaagcc taacgttcgc ttcgtggtgc attttgatat
     3781 tccgcgcaat atcgaatcct attatcagga aactggacgc gccgggcgtg atggcctgcc
     3841 cgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc gttgtctgga
     3901 agagaagccg caggggcagt tgcaggatat cgaacgtcat aaactcaatg cgatgggcgc
     3961 gtttgctgaa gcgcaaactt gccgtcgttt ggtattgctg aactattttg gcgaagggcg
     4021 tcaggagccg tgcgggaact gcgatatctg cctcgatccg ccaaaacagt acgatggttc
     4081 aaccgatgcg cagattgccc tttccaccat tggtcgtgtg aatcagcggt ttgggatggg
     4141 ttatgtggtg gaagtgattc gcggtgctaa taatcagcgt atccgcgact atggtcatga
     4201 caaactgaaa gtctatggca tgggccgtga taaaagccat gaacattggg tgagcgtgat
     4261 ccgccagctg attcacctcg gcctggtgac gcaaaatatt gcccagcatt ctgccctaca
     4321 actgacagag gctgcgcgtc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc
     4381 gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg
     4441 caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa gcaatgtccc
     4501 gccgtacgtg gtgtttaacg acgctacctt gattgagatg gctgaacaga tgccgatcac
     4561 cgccagcgaa atgctcagcg ttaacggcgt tggtatgcgc aagctggaac gctttggcaa
     4621 accgtttatg gcgctgattc gtgcgcatgt cgatggtgac gacgaagagt agtcagcagc
     4681 ataaaaaagt gccaggatga agactccgta aacgtttccc cccgcgagtc taatgtatgt
     4741 tgatgttatt cctcaccgtc gccatggtgc acattgtggc gcttatgagc cccggtcccg
     4801 atttcttttt tgtatctcag accgctgtta gtcgttcccg taaagaagcg atgatgggcg
     4861 tgctgggcat tacctgcggc gtaatggttt gggctgggat tgcgttgctt ggcctacatt
     4921 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
     4981 tctgctggat gggttaccag atgctacgtg gtgcactgaa aaaagaggtt gtttctgccc
     5041 ctgcgccaca agtcgaactg gcgaaaagcg ggcgcagttt tcttaagggc ttactgacca
     5101 atctcgcgaa tcctaaagcg attatctact ttggttcggt gttctcgctg tttgtcggtg
     5161 ataatgtcgg caccaccgaa cgctggggca ttttcgcgtt gattattgtc gaaacactgg
     5221 cgtggtttac cgtcgttgcc agcctgtttg ctctgccgca aatgcgctgt ggttatcaac
     5281 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
     5341 tgattatttc gcgttaagcg cttcccccct gatacgctat cagggggaat aacatcagga
     5401 ttgcaattga taaaggtctt cctgagaaag cccggtcatt tccataatca actgcaaatc
     5461 aaagccacgc tgtagcatgg cttttgcgac attgagaagg gcatcgcgcc gaagttcttc
     5521 cgctaaagtc atcagttcct ctccttgttg aggcactcgt tgtgccagac catgtactaa
     5581 tgttcgtaca tcctgtgctt cacctaccag cgccatgtag tttatcaaca tgcgtatttg
     5641 ctgtccgctc atctgcccca gaatcattaa gcgggataac ttatctaaca gcgttgtcat
     5701 atcccgttga cggatatgct tctgtaccag cgtaaaggcg gccatgctgc ggtgttgcat
     5761 gatttcgtca tcaggaatta gcgtaatatc aaccagcgga aaagcatgaa aatagagatc
     5821 gcgtgcaatg tccggatccg gaaaaccatc gagccagttt gttgaccatg gatatgggct
     5881 acgctttccc tggtaaaaaa gaatggggat caccagaggc agataatcat gtcccgcttc
     5941 aagatgccgc tgcatggtgg caatagcata gcgaagcata cggaacgcca tattttcatc
     6001 aggtgaactt tgatgctcaa tcaacacata gatatatccg caccctttcg tcgtctctac
     6061 tgaataaagt acgtcgttgt aatactggcg taggttttcc tctacaaaac tgctggactc
     6121 gagatggagc gtatcaagat tgcagagttt aactaaagaa ggcggcagat gaatttcaat
     6181 aaagtcacgg gccgtttctg gtcgggataa aaaagcctta aagattgcgt catgtggtgt
     6241 cgttgcttcc gtcatttttc atcatccgtg ggtgaaatat ggcggcattc tggcactaac
     6301 aaagagaagt aaatagtaaa taaattaaca gattacgctt attacgagat gcctcgagcg
     6361 cggatgtaac aatctctcga ggcacattaa tttacgtgag ctttattcca gttttaagca
     6421 tgcctcgccg atgctaacag cgctcccacc agcataaata acgagccgaa aatcttattc
     6481 agcgccttca tctgctttgg tcctttaatc catagagcaa tccgttgagc aagggtggcg
     6541 taaccgatca tcacaataat atcgaccaca atagtggtga cgccgagcac gatatactgc
     6601 atcagctgcg gctgttgcgg catgatgaat tgcggaaata gcgccgccag aaacacaata
     6661 cttttgggat tggtgagatt gacaaaaact gcgcgctgga acaaatgtcg acgcgattga
     6721 gtagaggcca gcgatttaag atcaattgcg ccagcggcgc gccactgctg gatcccaagc
     6781 caaatcaagt aagctgcgcc tgcccacttc aacacttcaa acgcaatcac tgagcgggaa
     6841 aatagcgtcc ccaaccccac gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc
     6901 ccagcaatag acgccaccgc gccgcgataa ccgtggttga gcgaggtggt catggtgttg
     6961 attgcgccag agcctggcga caggcttaaa atgatcgatg tcagcaggta ggcaaaccac
     7021 cattctaagg tcatgatgaa ctcccggtgt gtctattttt gtgccacaat acgctactgt
     7081 cgcagcgttg tgtcaggcac gcttaaaaaa acgattttac gtggtttaag aggcagatta
     7141 cccgatgttt cagcagcaaa aagactggga aacaagagaa aacgcgtttg ctgcttttac
     7201 catgggaccg ctgactgatt tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga
     7261 tgacattccg gttcgctttg tccgttttcg cgcacagcac catgaccggg tggtagttat
     7321 ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa ctggcctacg acctgttcca
     7381 ttcggggttt gatgttttaa tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt
     7441 agccgatcca catctcgggc atgttaatcg ctttaatgat tatgttgatg atctggcggc
     7501 attctggcaa caggaggttc agcccggtcc gtggcgtaaa cgctatatac tggcacattc
     7561 gatgggcggt gcgatctcca cattatttct gcaacgccat ccaggtgtat gtgacgccat
     7621 tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta tggcacggca
     7681 gatcctcaat tgggccgaag cgcatccacg tttccgtgat ggctatgcaa taggcaccgg
     7741 gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc cacagcagac agcgatatcg
     7801 acgtaactta cgtttctatg ctgatgaccc aacgattcgc gtcggtgggc cgacctacca
     7861 ttgggtacgc gaaagtattc tggctggcga acaggtgtta gccggtgcgg gtgatgacgc
     7921 cacgccaacg cttctcttac aagctgaaga ggaacgcgtg gtggataacc gcatgcacga
     7981 ccgtttttgt gaactacgca ccgctgcggg ccatcctgtc gaaggaggac ggccgttggt
     8041 aattaaaggt gcgtaccatg agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct
     8101 ccacgctatc gttgattttt tcaacaggca taactcaccc agcggaaacc gctctacaga
     8161 ggtttaaatt tcttatgtac caggttgttg cgtctgattt agatggcacg ttactttctc
     8221 ccgaccatac gttatcccct tacgccaaag aaactctgaa gctgctcacc gcgcgcggca
     8281 tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc
     8341 tggagattaa gtcttacatg attacctcca atggcgcgcg cgttcacgat ctggatggca
     8401 acctgatttt tgctcataac ctggatcgcg acattgccag cgatctgttt ggcgtagtca
     8461 acgacaatcc ggacatcatt actaacgttt atcgcgacga cgaatggttt atgaatcgcc
     8521 atcgcccgga agagatgcgc tttttcaaag aagcggtgtt caattatgcg ctgtatgagc
     8581 cgggattgct ggagccggaa ggcgtcagca aagtgttctt cacctgcgat tcccatgaaa
     8641 aacttctgcc actggagcaa gcgattaacg ctcgctgggg cgatcgcgtc aatgtcagtt
     8701 tctctacctt aacctgcctg gaagtcatgg ctggcggcgt ttcaaaaggc catgccctgg
     8761 aagcggtagc gaagaaactg ggttacagcc tgcaagattg tattgccttt ggcgatggaa
     8821 tgaacgatgc cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc agcgcgcatc
     8881 aacggctgaa agatctgcat ccagagctgg aagtgattgg tactaacgcc gaagacgcag
     8941 tgccgcatta tctgcgtaag ctctatttat cgtaatcgtt ctttatttgg tcagttgtca
     9001 acctgatact tcgctacaat ggatactcgt taatcaaaga gttttccatt gtggcgctac
     9061 ttatcatcac cacgattctg tgggccttct cctttagctt ttatggcgag taccttgcgg
     9121 ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg tctggcggca cttgtttttc
     9181 tgccgtttct gcgtacccgt ggcaatagcc tgaaaacggt cggcctgtat atgctggtgg
     9241 gcgcgatgca gctcggtgtg atgtatatgc tgagtttccg cgcctatctc tacctgacgg
     9301 tttctgaact gctgttgttc accgtactga cgccgctcta catcacgctg atttatgaca
     9361 tcatgagtaa gcgccgtctg cgctggggct atgcttttag cgccttgttg gcggtgattg
     9421 gtgccgggat tattcgctat gatcaggtca ccgaccattt ctggactggc ttgttgctgg
     9481 tgcaactctc caatatcact tttgccatcg gcatggtggg ttacaaacgt ctgatggaaa
     9541 ctcgcccgat gccgcagcat aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg
     9601 cagtgattgc atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat
     9661 ggggcattct ggtgtttctt ggcgtggtgg cttccgggat tggctacttt atgtggaact
     9721 acggcgcgac gcaggtggac gccggaacgc tgggcattat gaataatatg cacgttccgg
     9781 cagggctgct ggtaaacctg gctatctggc accaacagcc gcactggcca acgtttatta
     9841 caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc gctccgcgct
     9901 cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct gaacgaataa acgcttctgt
     9961 aatcggctga cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc acaagccttc
    10021 gcccagggtt ttggtgacca ccagaccctg gcgttcaaaa ctctccacca cccaatgagg
    10081 tagtgcggca atacccatcc gtgcggcaac catctgaatc aacaataagg tgttatcgac
    10141 gcttttcagt gacgggctga cgcctgctgg ctgaagaaaa tgccgccaga catccagtcg
    10201 gctacgctgc accggataaa tcaacagcgt ttcgctggcg agatcttccg gtgtaatacg
    10261 cgtttttgcc gccagtggat ggtcaggtgc taacaccaga cgtacttcat agtcgaacat
    10321 cggcgaataa tgcaggccac tgcgcggcag aatatcggac gtcatcacca gatccagctc
    10381 gccctgttgc aacgcaggct gcggatcaaa cgttacgccc gatttaaaat ccatctccac
    10441 ctgcggccag ttcttatgga aattttctaa tgcgggcgtc agccactgaa tacagctatg
    10501 gcactctatg gcaatgcgca ggcgcgtctg ctgcggttca ttgcaggctt gcagtgcctg
    10561 gctaatttgc ggcagcacct gatttgccag ttgcaacagg atttctccct gcggtgtaaa
    10621 gcgaagcggc tggcttttac gcacaaatag ccggaagcca aggcgttgtt ccagatcgct
    10681 aaactggtga gacagggcgg attgcgtttg atgcaacgtc gccgcagcgg ctgcgagtga
    10741 gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt acttcgatca tgaaagtcct
    10801 tcacttcggc atgaataatt tgcgcttgag gaatatacag taaccgccaa ttatggatgt
    10861 gtaaacatct ggacggctaa atactaaaaa ttccataagg ggcatataat gacaattctt
    10921 aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagag
    10981 agttattggg cggggaactc cacgcgtgaa gaactgctgg cggtagggcg tgaattgcgt
    11041 gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt
    11101 gcctggtacg atcatgtact gacaaccagt ctgctgctgg gtaatgttcc gcaacgtcat
    11161 cagaacaacg atggttcggt agatatcgat accctgttcc gtattggtcg tggacgtgca
    11221 ccgactggcg aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac
    11281 tacatggtgc cggagttcgt taaaggccaa cagttcaaac tgacctggac gcagctgctg
    11341 gaggaagtgg acgaggcgct ggcgctgggc cacaaggtga aacctgtgct gctggggccg
    11401 gttacctacc tgtggctggg taaagtgaaa ggtgaacagt ttgatcgcct gagcctgctg
    11461 aacgacattc tgccggttta tcagcaagtg ctggcagaac tagcgaaacg cggcattgag
    11521 tgggtacaga tcgacgaacc cgcgctagtg ctggaactgc cgcaggcgtg gctggatgca
    11581 tacaaacccg cttacgacgc gctccagggc caggtgaaac tgctgctgac cacctatttt
    11641 gaaggcgtaa cgccaaacct cgacacgatt actgcgctgc ctgttcaggg tctgcatgtc
    11701 gatcttgtac atggtaaaga tgacgttgct gaactgcaca agcgcctgcc ttctgactgg
    11761 ctgctgtctg cgggtcttat caatggtcgt aacgtctggc gcgccgatct taccgagaaa
    11821 tatgcgcaaa ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctca
    11881 ctgctgcaca gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc
    11941 tggtttgcct tcgccctgca aaaatgtcat gaactggcat tgctgcgcga tgcgctgaac
    12001 agtggtgata cggcagctct ggcagagtgg agcgctccga ttcaggcgcg tcgtcactct
    12061 actcgtgtac ataatccggc agtagaaaag cgtctggcgg cgatcaccgc ccaggacagt
    12121 cagcgtgcga atgtctatga agtgcgtgct gaagcccagc gtgcgcgttt taaactgccc
    12181 gcgtggccga ccaccacgat tggttccttc ccgcaaacca cggagattcg taccctgcgt
    12241 ctggatttta aaaagggtaa tatcgacgcc aataactacc gcacgggcat tgcggaacat
    12301 atcaagcagg ccattgttga gcaggaacgt ttgggactgg atgtgctggt acatggcgag
    12361 gccgagcgta atgacatggt ggaatacttt ggcgagcatc tggatggctt tgtctttacg
    12421 caaaacggtt gggtacagag ctacggttcc cgctgcgtga agccaccgat tgttattggt
    12481 gacgttagcc gcccggcacc gattaccgtg gagtgggcaa aatatgcgca atccctgact
    12541 gataaaccgg tgaaagggat gttgactggc ccggtgacta ttctctgctg gtcgttcccg
    12601 cgtgaagatg tcagccgtga aaccatcgcc aaacagattg cgctggcact gcgtgatgaa
    12661 gtggccgatc tggaagccgc tggaattggc atcattcaga ttgacgaacc ggcattgcgc
    12721 gaaggtttac cactgcgtcg cagcgactgg gatgcctatc tccagtgggg cgtggaggct
    12781 ttccgtatca acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttac
    12841 tgcgagttca acgacattat ggattcgatt gcggcgttgg acgcagacgt catcaccatc
    12901 gaaacctcgc gttccgacat ggagttgctg gagtcgtttg aagagtttga ttatccaaat
    12961 gaaatcggtc ctggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt
    13021 gaagccttgc tgaagaaagc ggcaaaacgc attccggcag agcgtctgtg ggtcaacccg
    13081 gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg
    13141 caggcggcgc agaatttgcg tcgggggtaa aatccaaacc gggtggtaat accacccggt
    13201 cttttctcat tacagcgacg tcttcccagc atactgcgta aaccattcca gatatcagtt
    13261 cataccctgt ttaagaaaat cttctttatt cccttctcat gaaataaatt tcgtcatttc
    13321 cgccattttt tctatctgcc tctccatc
//
LOCUS       DAJOTB010000129        13184 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJOTB010000129 DAJOTB010000000
VERSION     DAJOTB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.129"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..822)
                     /locus_tag="OHN28_003720"
     CDS             complement(<1..822)
                     /locus_tag="OHN28_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299033.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAAR"
     gene            complement(900..2123)
                     /locus_tag="OHN28_003721"
     CDS             complement(900..2123)
                     /locus_tag="OHN28_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2299034.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2149..2538)
                     /locus_tag="OHN28_003722"
     CDS             complement(2149..2538)
                     /locus_tag="OHN28_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HCQ2299035.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2555..3511)
                     /gene="arcC"
                     /locus_tag="OHN28_003723"
     CDS             complement(2555..3511)
                     /gene="arcC"
                     /locus_tag="OHN28_003723"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2299036.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3504..4928)
                     /locus_tag="OHN28_003724"
     CDS             complement(3504..4928)
                     /locus_tag="OHN28_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2299037.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(4925..6484)
                     /gene="fdrA"
                     /locus_tag="OHN28_003725"
     CDS             complement(4925..6484)
                     /gene="fdrA"
                     /locus_tag="OHN28_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2299038.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6579..7439)
                     /locus_tag="OHN28_003726"
     CDS             complement(6579..7439)
                     /locus_tag="OHN28_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2299039.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7445..8113)
                     /locus_tag="OHN28_003727"
     CDS             complement(7445..8113)
                     /locus_tag="OHN28_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCQ2299040.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8370..9179)
                     /locus_tag="OHN28_003728"
     CDS             complement(8370..9179)
                     /locus_tag="OHN28_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCQ2299041.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9441..10205
                     /gene="udp"
                     /locus_tag="OHN28_003729"
     CDS             9441..10205
                     /gene="udp"
                     /locus_tag="OHN28_003729"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCQ2299042.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(10240..11730)
                     /locus_tag="OHN28_003730"
     CDS             complement(10240..11730)
                     /locus_tag="OHN28_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HCQ2299043.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(11743..12249)
                     /locus_tag="OHN28_003731"
     CDS             complement(11743..12249)
                     /locus_tag="OHN28_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HCQ2299044.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(12266..>13184)
                     /locus_tag="OHN28_003732"
     CDS             complement(12266..>13184)
                     /locus_tag="OHN28_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HCQ2299045.1"
                     /translation="AALSIAPVQAASWPTKQIELVVPYAAGGGTDLVARAFADAAKNH
                     LPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVELTTLPSLGMVRFKTSDFKPI
                     ARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGNSGTGAIWHLAAAALEDKTG
                     AKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKGGKLKTLVVMADERMKTMPD
                     VPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKETAEEPAFQDALQKLNLNYA
                     WLDAASFQTQISEQEKYFDELLTRLGLKK"
BASE COUNT         3224 a         3451 c         3369 g         3140 t
ORIGIN      
        1 acgagcggca agaaataact tggtggcgtg tttatgttcg cgcattggct ttttaagcaa
       61 tttaccgctg ttcaataacg gtaatgccag atgatgtgga atataaccaa taccgacgtt
      121 ttgttctatt aaggcgattg ccgtagcgaa gtcaggaaca aaaatgggtt tttgtccttc
      181 caacaaccag gcttgcatcg gggggaagtt aacggaggta tcgcggatac aaaccgctgg
      241 ataatgacgc aattcactgt tttctaatgg atgccgcttc gctgccagtg ggtggagtgg
      301 gctgacgaca aaatcccatt ccatttgtcc gaccggttcg ctaatgatgc cttcactgct
      361 tggtactgca tgaggagcac cgtagaccag atcagcacgc ttactgtaca gcgcatccca
      421 gcagccgttg tatacctcaa gatctaccgt cagtgttgtt gatgtgaatt gccgttcaaa
      481 atctcgaata aatgccacca gcaaatcacc aggaataata ttgtttaccg caattgtcag
      541 ttcacgctca ataccatcgt gaatcagcac tgtgttgcgc tttaatgctt ccaggtcgcc
      601 aagaatagtt ttgctatgtt cgatgaagta acttcccgct ggcgataatt caatatagcg
      661 tcccttacga atgaacagtt caacgcccag gctctcttcc gctttcttga tggtgtaact
      721 gatggcggaa gggactttat ttaactgctc ggctgcggtg gtaatgctct ggtgtctggc
      781 gaccagatga ataacacgaa gcgtttcgtc tgagatgaac ataaggctcc ctggtcagtg
      841 gtaaagtggc tgaatctctc cagattcagc cgtaatcatt gcctgagtat acccaggaat
      901 tagcggtgtt gacttgcccg actgagaatc gcatacgcga tacatgccac cagaatcgag
      961 tctaccgaag ggatcgtcgt cagggtgaat aagcctttta cggtcataaa gccaaccgct
     1021 gcgcctatga tccatgctgc gaatgtcagc actttaatct gtgactcctg ggctaacact
     1081 gactcttgat agccattacg gcgataaagg aaaaagtcag cgatataaat tccggcaacg
     1141 ggtggcgtgg cgatacccag gaacagcaga aacggaataa accacgccat aatatccata
     1201 ctgcctacga tggcggaaag gatacccagc gccacggtaa tctgccattt gggaaagcgg
     1261 gtaagcagtg tggaaaccac cagcgtgccc tggaacatat tgcccgcgtt accggtgacg
     1321 caggcgaaaa tcaacaacag cgccgcaggc agaactgcac cgaataccgc catcgcaccc
     1381 agtaaagatc cctgaccact aatggcgctc ggcgttgcac ccgcccagta gagcagaggg
     1441 taggcaacca gaaacgtcac gcctgcggca atcagcgcat gtttgcggtt atgcacaaaa
     1501 ctaccgaaat caggcagtgt ggcaaccagc acaataatgg tgccaacaac ggtggaaacg
     1561 gcgacacctg tattcatctc cacaatcgcc gctggtactg ccacttcggt atgggttgcc
     1621 acatagagga tgtaacacag caccagcgcg ataaccggca cggcaacctg ggcaattttt
     1681 cccagaacgg caaagccaaa tgccgtagag gcgacaaaaa tgacacagcc agccgccact
     1741 aacagtgcca gtggaacttc cagtccatgt tgagccagta agtcatgtac cgaatgacca
     1801 aacatattgg cggtaacggc aatccagccg aacagactta tcgccatcaa aatgttaatg
     1861 gcgatggccc ctttttcgcc aaacgaatat ttcacaatgc catacgttgg caggcgggct
     1921 ttttctccca cgctaatggt gatggctgaa agtaaggtca gaatgacgcc gcctaacaat
     1981 acgacgctaa ttaaactgcc gctggaaagt tgattgccga ggcttgagga tgacaacagc
     2041 acaggtgtga cggcaatacc cagcaaaatc atggcaatac gcaatcctga tgccgtattc
     2101 agattctctt cttttttacg catagtatgt tccctttata aatgcgcgtt acacagtatg
     2161 aaaatgcccg gtgtatttac gccagctacc cagttctgta atttcccgtt ggccttcggt
     2221 cagccagttt tctgccagta cgcccagcac ttcgctacca tcggcaagtt tgattttgcc
     2281 aattgccagc ccagcgggtt cgctcattaa cagagcggca aaactggcca gcggtaattg
     2341 ccaaatttct acgtcgacgt gtgttcctcc ttcattcacg cgaatcattc ccggatggcg
     2401 atcattaata ctccacaacc ggtaatgggc gtcagttcgg tcttcacgga caaaaatacc
     2461 gcccgctttt tgcatatttg ggttgagttc caggccgcgc atcagcgttc cgttaacggc
     2521 taaaagcgtt gtagtcattg tgttctccag agaattaaag ggttatccat gtaccgctct
     2581 gatgcgccag ggctgctttt atggtttgcg gtgaagtaat caggccgctg gcctgtttgc
     2641 cttgttgctg gctggcattg atgaaatcaa caatggcttc cactttgggt tgcatactgc
     2701 cgacaccaaa ctgaccttcc cgcaacagcg tttgcgcttc ggcaacgctg atagcgtcga
     2761 gccactgttg ttgtggtgta ccaaagttaa tcgccacttt ttctacaccg gtggggatca
     2821 ccagcagatc tgcgcctaac tgactggcga gcagcgcgga ggccagatct ttatcgatca
     2881 cggcttctac acctttcagt tgttgcccgt ctcgcaccac cggaataccg ccgccgccgc
     2941 aggcaataac cagatatccc tggcgcacca ggtgagcgat ggtgtcgtgc tcaataattt
     3001 ccagtggtgc aggagaggga actgtacgcc gccaaccacg cccggcgtcc tccatcagcg
     3061 tccagccgag ttgttgttgg cgttgttggg ccgttgcttc atcgagaaac gcgccaatcg
     3121 gtttactggg gctggcgaaa gcatcgtcat gtgggctgac tcgcgtttgt gtcaccaggg
     3181 caattaccgg tttgtttatg ccacggcgag cgagttcgtt atgcagcgct ttctggaaca
     3241 tgtagccaat tgcaccttgt gtatcaccca cggcgtaatc aagtggaacc ggagaaactt
     3301 cgttactggc gagttcagag cggcgcagaa taaagcccac ctgcgggcca ttaccgtggg
     3361 ttagcaccag gtcccatccg gcttcaacca tatcaacgat atgctgcacg ctttccatca
     3421 ctgcaacata ttgatcggga atactattgc gttgctcgtc ctgaatgagc gcattgccgc
     3481 caaccgcgac gacagccagt ggcttaacca tgatggttct cctttgcgac gatctccgcc
     3541 agtgcacgta cagcggcgac aaaacaggcc atcggcgcag tggtgatccc ggcaccaatt
     3601 tgcccgaccc ccgcttgttt gtgggcgata cccgtattga tgaccggtaa aatgccgcga
     3661 tcggtcactt tacgcgcatc aatccctgct gcggtggggg caaaattgag ggctggaagt
     3721 gtaaatgcgg ggttaccgcc gagggtgatg gcttgcatcc gacggctgtt attggtggca
     3781 tcggcagggg tgcctcccac aaatttgacg atcgccggag acgacgccat cgcgaagcca
     3841 cccacacctg cggtttcagt aattgcgcta tcacccaggt cagcagcagc gtcgtcaacg
     3901 ccaaatccgg ggaagaacag accttcaacg gcattggcag gagcctggaa ccagcgatcg
     3961 ccagtaccgg aaatccgaat accaaaattc accccgttac gcgccatcac cgtaaccatt
     4021 gaactgaacg gtacattggc ggcggcatcc atcattgctt tacaggcggc catcgacaga
     4081 ttgaggaaga agtgatcgtt accggtgata aatgccacga cgcgctggat atgttcctgt
     4141 ggaagagagc aggccagcaa tgccgggagc aggcgcttaa tcagcaaacc ggtagcggca
     4201 gcattgcggt tatgaacttc atcccccata tgcagcgcct gcgccattaa tggcttgagt
     4261 tccagctcgc cgtgttgtgc gattgctgct ttcattgccg gcgtcagttc atcgcgcatc
     4321 caggccagac gattgagaac ttcatcgtta ttggcgccaa aacggagaac cttacccaga
     4381 ccttcattga aattgctaaa agtacggtgg ccgttggttt tgttttctac cacccataac
     4441 ggcatcgacg ggctgatgat gcctgccatt gggccaaccg cgtgataatg atgacaaggg
     4501 gcaaggtcga tttcgcctgc gtttatctgg ttttcagcgt cttgctggct ggtggcccaa
     4561 ccttcgtaca gcatggcacc aataatcgcc ccttttaccg gaccacacat ctcttcccag
     4621 gcaatcggcg ggccagagtg caaaatacgt ttttgttcag ccattgctgg aatggcctct
     4681 ttagcaagca tgacatccac cagcacgggc tgtgcttcga gataacgtgc catggcgatt
     4741 tggttggcat tttctaccag gggatgacgt agcaacgatg ccagatccaa tccagcgtca
     4801 atatcgcctt gtgctggtgg ctgccagttg agtgcaattg cgcttccccc ggcatgttga
     4861 atattgtcag caaaactgtg tagtcccgcg ttcacaacct tcaaaggctg attaaataag
     4921 gtattcatgc gtttagcccc tttttctgca actgagtctg tgcaacggtg ctggcccata
     4981 gcgccgcctg tgcattgctg ctggcgatga tgacccctgc attttgtagt gcgcttatct
     5041 gctgactgcg tatttgcgga tcagcttccg ttccgcaaac gtgcgcaatc agcaacggag
     5101 cgttattcat gtcgatgtgt gaaagttggt ccagtaattc gctggcaggc gtcgtggaag
     5161 cgccataacc aagtaccagg tcgaataaca cgacggcagt gtgtgagtcg ttgagttcgt
     5221 tgagcagacg ttgattgcgc agagtcgggt cgatcattgg atgcggtttg ccacgggtga
     5281 aatcatcgtc gcccatatcg atcaacgtgt gatcttcgct ttgccagata tttgccagag
     5341 cacgattacc cgcaactggc gtattggaag ctgcgctgaa tcccttttgt tggcagataa
     5401 gttgcgcttc gtagcagaag gttcctccgg cgaaaacacc gcgtatcgcc tggcgatggg
     5461 ctggcaggcg ctggcaggcg ttttgtaaca ttgtgagatc gtcgcagggt acatcggttt
     5521 cgacagccgg aagtggctga gcattcagta acgccacagc gatgtcggcg gcgctggcaa
     5581 gtgtggtggc ggcagtgata ttgggacgtg ccagatcatg cggattcgcc ccgaggaaat
     5641 tcaccaccac gggttttcca caagcctcgg cgcgctccag aatagtccgg gcaacgatcg
     5701 gtgaaggcgg ttttgaaatc agcacaatta cgcgagtttc atcgtcttgc gccagcgcat
     5761 ctaaagcgaa gagcatcgat attccaccaa tctctttact gagatcatgt ccgcctgttc
     5821 ccaacgcctg agaaataccc gctccgagct ggtcgattcg gcaagtcact tcctgtagcc
     5881 cggtgcctga cgcgccaata acaccaattg ctccgcgttt caccacatta gcgaagccta
     5941 acggaatgcc gttaacaatg gcggttccac agtcaggtcc cattacgatg cgctgacgtt
     6001 ctcgggcaag cgttttgatg gatttttcct gcacgatact gacgttatcg ctgaacatca
     6061 tcacgttcat tccgaggttt aacgctttaa ttgcttcggc ggcggcgtag tctcccggta
     6121 cagaaatgag tgccaggttg gcttcgggtt cggcttccag tgccatttcc aggctgacct
     6181 tttccggtgt tttaatgcca ctatccgttt catcatcggg tttgctggtc agacgttgct
     6241 gggccagtat cagtgcttca ttgcaaatat cttcttcacc catgactgca atcactaaat
     6301 cattgggact ggcgttgatt tcgttaccca gacccgcatc gcgcaattgt tcgagattat
     6361 tgggcgttcc catgacgacc gaggcctgtt gaataccggg caacttgctg atttgcgcgg
     6421 aaatctgcat cagagaaact gagtcctgat aaagattact gaaaacttta tatttaactt
     6481 tcatgctgtt acgcctgttt cattccgccc gatagcaaaa ttcgggcaaa gcatccccgt
     6541 gtagccccat aaacgggcta cgggagaaaa ggttgaaatc aattcatggt gttcagggtt
     6601 cgaaagccat gcagcattcc tgcaaggcaa tctactcctg acgttgcgcc aaattgcatg
     6661 acctgttctg cacaggatgc gattgtcatt gccgatgcgc tcccaaccag ttgtgcgagt
     6721 aactggcaaa tcggttctga aaaatgtccc tgaagcgcac gctccagata atgacgactg
     6781 atatccgtgg tgttgtgcgc atgttgatca attgcctgct gcaaccggta ttgatgatcg
     6841 gcaagtggtg cagggagttg ccataaccag agcgctgcca gatagcccaa caaatagtca
     6901 tctccatcgg gcgttaagcc tttaccaaag ccgatcagtt gtggcacctg gcgttccagc
     6961 gcatctggat gctccataat cgctaatccc tggtgcgtga gactatccgg caggtgcaat
     7021 gtgctttgaa cgttatgctc cagacaaaat aaaagccgtt gcgatgccat cgttgggtag
     7081 tgctggaaaa ccaaagggaa tgcctcaagt gtcagcattc cccccacaaa tggcgactgc
     7141 cagagcgttg cgttatcgag cgccacacaa aatcgaggtg tccgcagcaa gcctggggtt
     7201 aactgaatgg gatctgaatg ggcaatctct cttcgccagt cccaacattc aggtagccag
     7261 actctggcag catcagggag attcagatac gtttgcgcac acaacagagt gatgaatgtt
     7321 cccgcgtctg aatacagatt taccgcatgt ttaaaagcgc tatgtagacg taaatcttgc
     7381 gtttcatcct gcgggaaaag atatcccaac gacgatgcgc aaagcgtaac ctgtaccatc
     7441 ggcgttagcc ttccagacct gcaataatgg ctttggaatc agtaacccaa ccgaaaatgg
     7501 caccttgtgc tttgatcatc tcgagggcgt atttctggaa ttccgggaaa taggagccga
     7561 cacaatcttc cgggataatg cattcatagc cacgatcatt tgcttcgcgc acggtggtgg
     7621 ttacgcaaac ttcggttgtt acaccacaaa ctatcagcgt cttgataccg tggttttgca
     7681 aaatcagatg taagtcggtt tgataaaacg cgcctttgcc aggcttatca atcaccggtt
     7741 cgcctgccac cgggtacagt tcaggaatga tgtcatgacc tgcttcacct ctgaccagaa
     7801 tgcgtcccat cggtcctggt tcaccaataa aggtttttcc gccgcgcgtc agtttggctg
     7861 gcgggcagtc gcttaagtct gcacgatggc cttcacgagt atgaataacc ataattcctt
     7921 tttgacgtgc ggctgccagc acctctgtac atggtgcgat agccgtgcgt acccgtgaaa
     7981 catcattgcc cagtgcctca ccgaagcccc cggcttcgac aaagtcacgc tgcatgtcga
     8041 tcatgaccag tgcagttgtg cgcggatcaa aaggtaattc aaagggttga gcctgaaaaa
     8101 tggattgcgt catggtaaat ctcctgtctg atgtttaaac gatccagttg ttctgagaac
     8161 gagtattcag gagaaaagcg gcacaaaaca ggaaaaaagt ttgccgcaca gcaacgaaat
     8221 ttttgcattc agcaggtggt gtgatgacga atgtgaaggg agtcaaagag tggcatacag
     8281 caacaaattt tttgatgctg tatggttttg agattaaaaa aatcgttgaa attgtgtgag
     8341 cttaatcatc agatttagcg actatggcgt tactttttaa caccatactg tgtaaaccat
     8401 tccaacattc gctgccagcc gtctttcgca gactcggcat gatagctcgg acgataatcg
     8461 gcgttgaacg catgctctgc atccgggtac acgataatct ctgctttcgc gtttgctgcc
     8521 cgcagcgcct ggcgcatcgt ttccacactc tcttgcggaa tgctgttatc ctggccgcca
     8581 tataagccga gaaccggcgc gttaagatcg gttgcgatat caacaggttg tttcggtgaa
     8641 ttcagcgact tatcgcccgt cagtttgccg taccacgcca ctgcggcttt tagctgtgga
     8701 ttatgcgcgg catacagcca ggtgatacgt ccaccccagc agaatccggt gatcattaaa
     8761 cgatgaacat cgccgccgtt gcgggaagcc caactggcga catgatcgag atcggccagc
     8821 acctgcgagt caggcacttt tgctaccaga ccgccaagca acgtggggat atcggcaaaa
     8881 tcattcggat cgccttcgcg gaagtaaagt tcaggtgcga tagccagata cccctccagc
     8941 gccagacggc gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc ctgcactaca
     9001 atgaccactg gcagtgggcc atcgctttgc tttggtctgg catggtaagc aggcatgtta
     9061 tccccttgtg aagggataga ggtgaagccc gccacaatcg cgtcgtccgg agtctgaacg
     9121 atggtcgaag cgagaggcga tgcagcaggt gcaaatccag attgttgtgt tgttgccatg
     9181 gtattctccg tacccttata aaaatgttgc gcaatgttaa ctatagtcag catgcaacaa
     9241 atcacattgc ctgaatcggc tcatctttta tgcagtcctg cagaatgaag agtgatttat
     9301 gtgatttgca tcacttttga tgggtaaatt tatgcaacac attttcgtca tggtgatgag
     9361 tatcacgaaa aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc tgactaaacc
     9421 gattcacaga ggagttgtat atgtccaagt ctgatgtttt tcatctcggc ctcactaaaa
     9481 acgatttaca aggggctacg cttgccatcg tccctggcga cccggatcgt gtggaaaaga
     9541 tcgccgcgct gatggataag ccggttaagc tggcatctca ccgcgaattc accacctggc
     9601 gtgcagagct ggatggtaaa cctgttatcg tctgctctac cggtatcggc ggcccgtcta
     9661 cctctattgc tgttgaagag ctggcacagc tgggcattcg caccttcctg cgtatcggta
     9721 caacgggcgc tattcagccg catattaatg tgggtgatgt cctggttacc acggcgtctg
     9781 ttcgtctgga tggcgcgagc ctgcacttcg caccgctgga attcccggct gtcgctgatt
     9841 tcgaatgtac gactgcgctg gttgaagctg cgaaatccat tggcgcgacg actcacgttg
     9901 gcgtgacagc ttcttctgat accttctacc caggtcagga acgttacgat acttactctg
     9961 gtcgcgtagt tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg ggcgtaatga
    10021 actatgaaat ggaatctgca accctgctga ccatgtgtgc aagtcagggc ctgcgtgccg
    10081 gtatggtagc gggggttatc gttaaccgca cccagcaaga gatcccgaat gctgagacga
    10141 tgaaacaaac cgaaagccat gcggtgaaaa tcgtggtgga agcggcgcgt cgtctgctga
    10201 aataaaaaaa cgctccccaa cttcggggag cgatatcagt tattgacgcc gtaaacgcat
    10261 tattttgcgg attattggcg tcagtagcgt aaaggctgcc agagtcagca atgccagagt
    10321 gatcggtcgg gtatacagaa agtcgagtga cccaccagaa agcgacagcg cacggcgaaa
    10381 attactttcc gccattggcc ccataatcaa tgccagcact acgggtgatg ccggatatcc
    10441 tcctttctgc atgaaatagc ccaccacgcc tgcaatcagc ataatgccaa catcaaaaag
    10501 gctattgttc aacgcataag tcccgaccac acaaagtgcg aggatcatcg gtgtgagaat
    10561 attgccggga attttcacca cattaccgat gactttcaaa gagagcagtc caagaactag
    10621 tagcatgaag taacagaaga tcatgccgac aaacagtgta taaaccatat cgccgtggtc
    10681 ggtaaacatc agcgggcctg gttgcattcc ctgtaacgtt aacgcgccca gcatgatggc
    10741 ggttaccgca tcgccaggga tccccagcgt cagcataggt aacaatgcgc caccggtgca
    10801 gccattcgcg cctgcctcac aggaggccac agctttaatt tcacccttac caaagttttc
    10861 tggtgtctta ctgaaacgac gagtttcgtt ataggcaaca aaagctgcaa tatccgcccc
    10921 cgcaccgggg atcatgccga taaaaacccc taatcctgat gagcgcaaaa tggttaatgc
    10981 gatgcggcgt agtgtttgcc agggcagtaa cagactgccg atagccacct tcgccccctg
    11041 acgaatttgc tggttttgct ccatagagcg gaatgcttct gaagcagcaa ataaaccaat
    11101 catcaccggg ataaatggca cattcatcag ctccacaaat ccacctgtaa accgtggaaa
    11161 accgcccatt ggatcaaggc ctatcgttgc gatcagcaat ccgccaaccc cggcaatcag
    11221 ccccttaatc agcgactcac cggaaatact ggcaataatg ctcagtccga aggttgccag
    11281 ggcgaaggac tccgaggcac taaatttcag cgcccaactg gcaagtaccg gagagagaaa
    11341 catcagcacg ataatgctga gcgttccgcc actaaaggaa gagaacgtgg cggcactcaa
    11401 tgccagccct gcttttccct gcttcgtcag ctcatatccg tcaatggcgg tggctgcggc
    11461 ggcaggcgta ccgggaattt tgagcaaaat tgcggtgata gaaccgccat agacgccgcc
    11521 aaaaaagacg ccgcaaatca tcaacaagcc agaaacaggc tccatgccgt aggtgaaagg
    11581 cagcaaaatc gccacaccca tggtcgctgt cagaccaggc agagcgccaa tcacaatacc
    11641 cagcatcacc ccaaaagtgg caaacagcag cgcctgagga ttattgagta atgtcgaaaa
    11701 accctgagtt aaaagttcac tttcaaacat tgctggcccc tgttattcga aaaatgtgcc
    11761 gacaggcaat ggcacctgta acgcttcgac aaacagcaac cagaggattg ccgttaacag
    11821 cagcgcaata cccggtgtga gtacccatcg cctgtagccc atcaacgtca taatgacgat
    11881 gaggaaaaca ggagtagtaa tgagataacc gcagtagctc atcgccacaa tataaaacgc
    11941 ggttgccgca attcctgttg ccgttttcct gaatgccggt ttttcctgtg cgttggcgca
    12001 agaggctggt tttgcctgca aaagatcgcg tatcaccagc aagacagcga gcacaatcag
    12061 ggcacaggca tagaaggtag ggaaggcact tgcaccggga tccgtttcaa tggcagttgc
    12121 tggaaaatca gcggcattaa tcatcaccgc gccagcaacc acacaaagta acagcgccac
    12181 caccatcgtt gatgaaaggc gcttgcggtg cggtacggcg agttgagcaa cagactgttt
    12241 tgtgttcatc aaccgtacct caggtttatt ttttcaggcc caggcgagtc agcaactcgt
    12301 caaagtactt ttcctgttcg ctgatttggg tctggaagct ggcagcgtca agccatgcat
    12361 agttgagatt caacttttgt agtgcatcct ggaatgcagg ctcttcagcc gtctcttttg
    12421 ctgcctttgc cagaacatcc accacatcct gcggcgtttt ttgcgacaca atcaggccgc
    12481 gccaggtgcc gatggagaga tcaacgcctt tctcttttaa cgtcgggacg tcaggcatgg
    12541 ttttcattcg ctcatccgcc atcactacca gtgtcttcag cttgccgcct ttcacatggt
    12601 tgataacttc tcctgggctt acggaaaccg cttcaatgtg cccgcctaac aggcctgtaa
    12661 tggcaggggc tgcgccgtca tacgggacat gagaaaactt tgcgcccgtt ttgtcttcca
    12721 gtgcagctgc cgccagatgc cagatagctc cggtgcctga gttaccaatg cgtacttttc
    12781 cgggattcgc tttggcgtaa gtcataaatt cttcatagct attccacggc gcatcggcac
    12841 ggactgtgat agcagccgga tccgcattca gacgggcaat gggtttaaag tcgctggttt
    12901 taaaacgcac cattccgagg ctgggaaggg tggtcagttc aaccgtgcct aaaccaattt
    12961 tgtaaccgcc agggcgggca gcggcgattt cactcaggcc gatagcaccg cctccgccag
    13021 gtttattgat aaccccgatg ctgacgggta aatggttttt ggcggcatca gcaaaggcac
    13081 gggcaaccag atccgtaccg cctccggcag cgtagggaac gactaactcg atctgtttgg
    13141 ttggccagga ggcagcctgt acaggcgcaa tgctgagggc agcg
//
LOCUS       DAJOTB010000130        13051 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJOTB010000130 DAJOTB010000000
VERSION     DAJOTB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.130"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            506..1281
                     /locus_tag="OHN28_003733"
                     /pseudo
     CDS             506..1281
                     /locus_tag="OHN28_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(1313..2474)
                     /locus_tag="OHN28_003734"
                     /pseudo
     CDS             complement(1313..2474)
                     /locus_tag="OHN28_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2642..3622
                     /locus_tag="OHN28_003735"
     CDS             2642..3622
                     /locus_tag="OHN28_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCQ2299046.1"
                     /translation="MTLLVTAECVDDLINDVHQAILADGLSNDPTKGSTLEILGAYLV
                     LTDPRRRISRTESRGKLISCLGEFLWYLSAQNDLEFIKHFIPRYAESAESDGKIHGGY
                     GPRLFNMRGLYNQLKNVINLLQEKKPTRRAVIQLFDASDLVPSKTEQREYVDIPCTIS
                     MQFIVRCEELHLFVCMRSNDAFMGLPHDVFAFTMLQELVVRLIGCELGHYHHFVSSLH
                     LYKNDLEKVKALQKEGFMSTLPVMGSMPEMHSLDDILPLLEAEKALRENKEFDIDTTS
                     LNDYWKDLLRILKIHFLLRSHTPNHKGLAFQELAAMKNQEYRFIFEGRLQ"
     gene            3619..4563
                     /locus_tag="OHN28_003736"
     CDS             3619..4563
                     /locus_tag="OHN28_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299047.1"
                     /translation="MKRHNQISQLVSNLQNFSRNEHNLNGLSSPACFDVLACQIIDSI
                     RRIRYVETLALRTDYMTPLRKEPNSDVFDPLRAACLYLRDNNYDEACWLVFLATHFGK
                     SNKTGWILCRDIYSGLGTQTWTWDTITDDFAAFEQWFASVSDELTANSSLRQYGNHRK
                     YETKKYHSRRSIPAVFRSYIGFIGATHSHEARFAEAKSFSSSPESLFELLYSGLNAVI
                     SFGRTAKFDYLTMLKKTGLLDVEPGHAFLNGATGPLQGSRLLFSNSRTAGDTIDVLNE
                     KLADLAAIIPAPYLRMQVIEDALCNWQKSPDRYVYFGG"
     gene            complement(4566..5648)
                     /locus_tag="OHN28_003737"
     CDS             complement(4566..5648)
                     /locus_tag="OHN28_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCQ2299048.1"
                     /translation="MIVTQDFQPDWVSPPGDTIIDLMDEHGLSGEELSKRIGLSLIRG
                     QQLLEGKIRLNESLACKLEELFNVSTDFWIKREVAYRQQIGYLEKVNNDWLESLPVRD
                     MMEFGWIPKTTTRESKFQHCLSFFGINSVNDFFYKIQSDAPLVAFRKSLNLATEPMAD
                     LVWLTRAAQLTSNQNCAVWDKEGLIALLPELRKATNEPDMRIFIPYIRDLLATAGVSL
                     VVLPTPKGCRASGATCFFTPDRATLVMSFRYLTDDHFWFTLFHEIGHLILHEAVRVRV
                     EGKDSLSSEEEKEADKFAVDVLIPEEYRQQLSRYGVRHWKEIIRLARKMGVSKGIVLG
                     YLQHEGNIPFSHLNRFKVRFRKEDIV"
     gene            6115..6381
                     /locus_tag="OHN28_003738"
     CDS             6115..6381
                     /locus_tag="OHN28_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCQ2299049.1"
                     /translation="MKGKHQDTKALSDVLAEMQRQDAKWGADRNQDPFIWGAILGEEV
                     GEFHQAVLHDRFGGKAAGTSREEAVQIAAVALQIIEYYDRVIDR"
     gene            complement(6681..7195)
                     /locus_tag="OHN28_003739"
                     /pseudo
     CDS             complement(6681..7195)
                     /locus_tag="OHN28_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7803..11216
                     /locus_tag="OHN28_003740"
     CDS             7803..11216
                     /locus_tag="OHN28_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCQ2299050.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYDQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTENRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            11280..12914
                     /locus_tag="OHN28_003741"
     CDS             11280..12914
                     /locus_tag="OHN28_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCQ2299051.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
BASE COUNT         3593 a         3075 c         3146 g         3237 t
ORIGIN      
        1 agttatgaaa tcataaagaa aaattattaa tgtgataaaa tgtgatatgt tctcaggttt
       61 tgtgatttta ttttataatt ctggtgattg tgttggcgtt atcacacgct cgaaatgaag
      121 ttatgcataa ccgctgcata attacctgtg tatgattaac aatgatctaa cacccttccc
      181 gataaacatg agctcgctta gatgtcacag gaaaagggct agtatggcag cactggtttt
      241 cgtatgaaag aaagataaaa cattgcctta tatctaaggc ttcttgatta tcggtaacat
      301 ccaccatctc cagccatagc tggcaggtgg tgacgttggt gcagtactgg cgggagcttc
      361 ggctccggaa ctggcatacc tgctgaaatc aacagaaaaa gacccggcag taaaggccat
      421 tgctcacgcc attctgggcg gtgcagtagc ggcaatgcag agtaataatg ttgctgctgg
      481 tgcagcaggc gcagcaacgg gagagctggc agccagagcg atcggaatgc tgtatccggg
      541 agtgaagctg tctgacctca gtgaagagca gaagcagacg ataagtacgc tggcaaccgt
      601 gtcagcaggg ctgaccggcg gtctaaccgg aaacagcacc gcatcagctg cagtgggagc
      661 acagagcggg aagaatgctg ttgagaataa ctacctgagc gtgtctgaaa agacagaact
      721 tgagatagca aaacagacga ttaaaaacag taaagatccg gcagagcgtg aaaaggcaca
      781 gcagaaatat gatgcattgc ttgaaaaaga tattgccagt gataaggagg ttattgctgc
      841 ctgtagtaat ggtaatgcgg gtagttcagc gtgtgccagc gcaagactga aagctattgc
      901 gtcgaaagaa gggtacgaag atggccctta taactcgaaa tacagccaac aatacgctga
      961 tgcgtatggt cagatagtca atctcctcga tatcaccagt gttgatgcac agaatcagca
     1021 gcaggtgaaa aatgtcatga ttaactattt catggtcacc aaaggggtgg atcgtcagac
     1081 cgcagaaagc tacaccgaaa caaagcaggg cttggagatc attgcggctt cggtaacgcc
     1141 tcttataggg caagctgcat caaataaatt aagctacctt ggtattggga aaaagatatc
     1201 ctttgatggt gattttatac tgtagatgga atgaaattca gcaaaagtta ttatgagaaa
     1261 ttatgggcgc aaggcagacc tgtgatccta cccacgtaat atggacacag gcctaagcga
     1321 ggttctggtt ttcaaattgt tccggactga ggccgccaca ccaactgtgc cgccgccacc
     1381 gattgtaatc acattcgata taattaaaca ccgttgcccg cattatttcc cggctgataa
     1441 agtgttctcc atggatacat tccactttca gcgaatgaaa gaagctttcc acgcaggcat
     1501 tatcgtagca gcaacctttt gcgctcatac ttccacgcag attatgccgc ttcagttgcg
     1561 cctgataatc tgctgaacag tactggcctc cacggtccgt gtgaacgata acgttccggg
     1621 gcctcttacg ccgccacagc gccatctgca gggcatcgca ggccagttgc gccgtcatgc
     1681 gtggcgacat tgaccagcca ataacggcac gtgaccacag gtcaatgacc actgccagat
     1741 acagccagcc ttcatctgta cgtaagtacg tgatgtctcc tgcccacttc tggttcgggc
     1801 cactggcgta aaaatcctgc tccaacagat tttctgacac aggcaggccg tgtgcgcggt
     1861 agctgaccgg gctgaacttc cgggaggcct ttgccctcag tccctgacgg cgcaggcttg
     1921 ccgccacggt ttttacgtta aaggggtaac cctgatcacg cagttcatcc gtcaggcgtg
     1981 gggcaccgta acgctgtttt gaccgggtaa aagccgcgag gacaacgctg tcgcagtgtt
     2041 ggcggaactg ctgacgcgtg cttatccttg tccgccgctg acaccacgta taccagccgc
     2101 tgcgggccac ccggagcacg cggcacattg ctttgatgct gaactcagcc tgatgttttt
     2161 caataaagac atacttcatt tcagacgatt cgcaaagtat gtcgcggcct tttggaggat
     2221 agccagttcc tctgcctgct ccgccagttg ccgcttcagg cgggcatttt cagcggccag
     2281 ttcactttca cgctctgacg aactcatttg ttgctgcagt ttgctgcgcc acgcatagag
     2341 ctgtgattca tacaggctga gttcacgggc tgcggcagca acaccgatgc gttcagcaag
     2401 tttcagggct tcactgagaa attcaggcga atgctgttta cggggttttt actggttgat
     2461 actgtttttg tcatgtgagt cacctctgac tgagagttta ctcacttagc cgcgtgtcca
     2521 ctattgctgg gtaagatcaa gtcttactta ccctgtgaac catgttgata ttgatactat
     2581 attgactata tgccatacag gtggtaatga ttagttacta ccatacagga aaaggtagaa
     2641 aatgactctt ttggtaacgg ctgagtgtgt tgacgatctg ataaacgatg ttcatcaagc
     2701 tatcctagcg gatggactca gtaacgatcc tacaaaagga tcaactctag agattcttgg
     2761 tgcctatctt gtccttaccg acccccgccg aagaataagt agaacagaat cccgaggaaa
     2821 actaattagt tgcttaggtg aatttctgtg gtatctctca gctcagaacg atcttgagtt
     2881 tataaaacac ttcataccac gttacgctga atccgctgag tcagatggga aaatacatgg
     2941 tggttacggg ccacggttgt ttaatatgcg tggtttatat aatcagctaa aaaatgttat
     3001 caaccttttg caggagaaaa aacctaccag acgtgcagta atacagcttt tcgatgcctc
     3061 agacttggtg ccctcaaaaa ctgaacagcg agaatacgta gatattcctt gtacaatatc
     3121 tatgcagttt attgtcagat gtgaagagct gcatctattt gtttgtatgc gttctaacga
     3181 tgcctttatg gggctgcctc atgatgtatt tgcgttcacg atgctacagg aattagttgt
     3241 ccgcttgata ggatgcgaat tgggtcacta tcatcacttt gtatccagcc tacatctcta
     3301 taaaaatgat ttagaaaaag tgaaggctct tcagaaagag gggttcatga gcaccctacc
     3361 agtcatggga tcgatgcctg aaatgcattc cttagatgat atcttacccc tgcttgaggc
     3421 agaaaaagct cttcgagaga ataaggaatt tgatattgac actacttcct tgaatgacta
     3481 ctggaaggat ttattgcgta tattaaaaat ccactttttg ttacgttcac atacacccaa
     3541 ccacaaagga ctagccttcc aagagttggc tgcaatgaaa aatcaggaat atcggtttat
     3601 ttttgaagga agactacaat gaaaagacat aatcaaatca gtcagctggt tagtaatttg
     3661 caaaatttta gccgcaatga acacaaccta aatggcctaa gttctcctgc ctgctttgat
     3721 gttttagcct gtcagattat cgacagtata cgtcgtatac gctatgtgga aactcttgca
     3781 ttacggaccg attatatgac tccgttacga aaagagccga attctgatgt ttttgatcct
     3841 ctccgcgctg cctgccttta tcttcgtgac aacaattacg atgaagcctg ctggctggtt
     3901 ttccttgcca cacatttcgg caagagtaac aaaaccggct ggattttgtg tcgagatata
     3961 tacagtggac tcggtacaca aacgtggaca tgggatacga taactgatga ctttgctgct
     4021 tttgaacaat ggttcgccag cgtctctgat gagttgacag ctaacagtag cctaagacaa
     4081 tatggtaacc atcggaaata tgaaacgaag aaatatcact ctcgtcgttc aatacctgcg
     4141 gtttttcgct cttacatcgg attcattggt gctacacaca gtcatgaggc taggtttgca
     4201 gaggccaaga gcttttcatc ttcaccagaa tctttattcg aactattgta ttcagggcta
     4261 aatgccgtga tctcatttgg acgaaccgca aaatttgatt acctaactat gctcaagaaa
     4321 acgggacttc tggatgtaga gccgggtcat gcttttctga atggagcaac cgggcccctt
     4381 caaggaagcc ggctactttt cagcaatagc cggactgctg gtgacacaat agacgtattg
     4441 aatgaaaaac tagctgacct tgcggcaatt attcctgcac cctacttgag aatgcaggtt
     4501 attgaagatg cgttgtgcaa ctggcagaaa tcacctgacc gttatgtcta tttcggcggc
     4561 tgatatcaga cgatatcttc tttacggaat ctcactttaa accgattaag gtgagaaaaa
     4621 ggtatattac cttcatgctg caaatatccc agtacgatcc ctttggatac ccccattttc
     4681 ctagctaacc ggatgatctc tttccaatgc cggacaccat agcgactaag ttgctgccgg
     4741 tattcttctg ggataagaac atcaacggca aatttatctg cttccttttc ctcttccgac
     4801 gaaagactat ctttaccttc tacgcgaact cggacagctt catgcaagat gagatgccca
     4861 atctcatgaa acagcgtaaa ccaaaaatgg tcatcggtca gataacggaa actcatgact
     4921 agggttgcgc gatctggtgt aaaaaagcag gtagcaccac ttgcccgaca tccctttggc
     4981 gttggcaaca caacgagaga aactccagca gtggcgagaa ggtcgcgaat gtaagggata
     5041 aaaatcctca tgtcgggttc atttgtagct ttgcgcagtt ctggcaacaa tgctatcagc
     5101 ccctctttat cccatacagc acagttttgg tttgaggtaa gttgtgcagc cctagtcagc
     5161 cacaccaaat cggccatagg ctcggttgcc agattaagag atttacgaaa agcgaccaaa
     5221 ggagcatctg actgaatttt ataaaaaaaa tcattgacgg aatttattcc gaaaaacgat
     5281 aggcagtgct ggaatttact ttcccgagtt gtagttttgg ggatccaacc aaactccatc
     5341 atatcacgta caggcaatga ttcgagccaa tcattattca ctttctcgag gtatccaatt
     5401 tgctggcgat aagcgacttc gcgcttgatc cagaagtctg tcgatacgtt gaataactcc
     5461 tcaagcttac aagccagaga ctcattgagt cggatttttc cttcaagaag ttgctgaccc
     5521 cggataagag acaatccaat cctcttactc agttcttcac cagaaagccc atgttcatcc
     5581 attagatcta taattgtatc accgggtgga gatacccagt caggctggaa gtcctgcgta
     5641 actatcattt cacgtcacct atgaacacta accgtatccg gctaactcgg gaccagttaa
     5701 ttgtattatc ttgatttttg ggctgaactg tattcccaca gacaaatccc atttttacat
     5761 caccgagaat ggtaattgta atacactctt ccccatcctc aaagcagacc tccggcgaac
     5821 ctacgggcaa cagtaatatg ttgtctgtca cggaaagatc ggaaagtcgc gagcgtagtc
     5881 ttgatgccac tttgtcacca aagatctcat ttgcctcatg catactctcg caaatagtcc
     5941 ttaacttctt gctttgaaag gtaatgaaca aaattatcca cttttatttt tcttggttgc
     6001 tacgtttata gcaaactcaa cgctcattca caaaatgaga tgtagcgaat taatggactc
     6061 tgcacatatt aaatgttaat gatggactta aaatcacgga ttcgtaaggg aaacatgaaa
     6121 ggtaaacatc aagacactaa agccctgtct gacgtgctag cggaaatgca acggcaggat
     6181 gccaaatggg gggcagaccg taatcaagat ccttttatct ggggagcgat tctcggagaa
     6241 gaggtgggag aatttcatca ggctgtcctc catgatcgtt ttggtggtaa agcagccggt
     6301 accagtaggg aggaagcagt acaaattgct gctgttgcac tgcaaatcat cgaatattat
     6361 gatcgcgtaa tagatcgata aattagaatt aatcaactaa gagtctttta cacgcgaaga
     6421 tgaactctgg cagccatcta ccttcgtggg cctgtagaac acatctaatt gcaatactcc
     6481 cagttaatca ctaaccagcc tctccagtaa ggctggtcga ttaggtttaa ttaaacagat
     6541 ataacaagca attcacttgg tattacgcgg ttcacacaga cttctttagt tgtgtcatat
     6601 cctataactt tcagaggctt atttacggta ttatcacgaa cggtgcaaaa gtggctcttc
     6661 cgcaacctag gtggagattt tcatgccagt ggactcatta ccctctgcct aagtagttca
     6721 aatccggctc gaccatacat cagatgtttc agcatcttca ggcgatttac atgaccttcg
     6781 acaacaccgt tactccagcg gctgctggtt gcctcacata tagccgcagc atcagcttcc
     6841 atccccgcag ccacgcgccg aagttctgct gaaccgtttt cgtggactcg agtgaaccaa
     6901 ctgcttagat ttgatttatt tggggttttc agaatacggt agaactcaag tactagttgc
     6961 tgcgctattt tcagcgccgg ttctttttca cacatcagac taataaatcg ggaagcataa
     7021 ttttcttcac ccctgattat tatccagggc atcaaccagc ggcttacccg ggacactgaa
     7081 ggaagtctgg cggcagtagt aactggtggg ttccagcctt tacgccattt ggcaacagca
     7141 tccctgacga tggtttcact gcctgtaacc cccgggccac catttcccgc catatccagt
     7201 cgctggtgtg ctgaattggt catgtatgaa ggaagatatc aatggtggta tctccctttc
     7261 aaaaggaagt gaaaagcttg atgcctgctg ccaggcaatc aatattacgt gcttttaacg
     7321 actatcctaa atggtggtag ctatgcagag ctcccggtcc tagcgctggg agcttatcgc
     7381 ttatctttac cgtaagcgta aacggaccgc cgtatgtagc cattaaacca gtattggtaa
     7441 cgtagatacc caccttttca aaagggaagt ggataaaaga tagcgaaaat gcgaagagtt
     7501 gattaatgca ttgaaacaaa attcattaat gatgaacttg gagaaaagct ccatcgtttt
     7561 attggctcaa aaaacatatc ccatagatga tcagccatac cttcattggt tagcaaggta
     7621 tcctgtgagt agccatggct gaagatgccc attgatgggc gaaaaccaag gatctgaccg
     7681 ttagagcagt tatgtcgtta aacacatcac caccatatgc catccggctc acttcgtcgg
     7741 ccaaattagt gtgatattct ttccagaaat gctgttgtat ccacgacata aggcgatgat
     7801 tgatggagca gtctctcaat tttgaaatgt tgcgcagcca gtggccggaa ctggctgaac
     7861 tcgcgtgtat ggcggaacgc tatgttcact ccgatccgga aagttgtctg gtcaagctgc
     7921 gcaactacac cgaattgatg gtgcgctggt tgtatcgtca ggagcggttg ccggaaggta
     7981 ttaaggctaa tctttacgat ttaatgaatg ctgatgtctt taccagcatg atgccggaag
     8041 ccatcatcat gaaaatggat gcgctgcgta tccatggcaa ccgtgccgcg cacggcggac
     8101 gtatcaaagc taaagatact tactggctgc tcaaagaagc gtatttgttg ggaatttggc
     8161 tgtatgttcg ctacgcccac ggtaatgttg atgactgccc caaatttaca ctccctccat
     8221 taacacaatc ttcaggtcgt gcagatgaaa aacgtctgga agatgcaatc agggctcagg
     8281 atgaaagccg tgagcgcgaa ctggcgctgc aacgcgcact acagcaagaa caggaaaagg
     8341 ccgaacacct cactcaacgc ctgaatgaag ccagagcgcg taaccagcat gttgccgata
     8401 ttctctctat tgatgaagcg gaaacccgcc gtcgcctgat tgactctcgc ctgcttgctg
     8461 ctgactggaa tgtaggcgaa ggccttaaaa ataccgatca ggttacgcag gaacatcccg
     8521 ttaaagaaca acctaccgcg accggagacg gttatgcgga ctatgtcttg tgggatgagg
     8581 cacacaaacc gctggcggtg gtggaagcaa aaaaaaccag cgtcaatgcc gagcaaggac
     8641 gaattcaggc ccggctgtat gcagactggc tggaaaaaga atacgaccag cgtccgatca
     8701 tcttctacac caacggctat gatatctggc tgtgggatga ccataaaact catggttatc
     8761 ccccgcgtcg ggtgtttggc ttctacagca aggaaagcct gcaatatctt attcaacagc
     8821 gtgaaacccg tcttccgctg aacagtgtgc cgcacgtaaa agataacgaa ggtaaggccg
     8881 ttgccggacg tttgtaccag cttgaaacca ttgcccgtgt cagcgagcgg tttaccaaca
     8941 aataccggca atcgctaatt gttcaggcta cgggtacagg caaaacccgc gtggcaatag
     9001 cgctaagcaa actaatgatt gatgcccgct gggtaaaacg cgtcctgttt ctttgcgacc
     9061 gcaaggaact acgtaaacag gcggcgaatg cctttaatca attcaccaat gagccgctgt
     9121 atgtggtcgg gaaatcgaaa aaagcagaca ggcagaatgc taggatctac atcgccacct
     9181 atcctggtat gatgaaaatc atggaccatt tcgatgttgg ttatttcgat ttgatcatcg
     9241 ccgatgaatc ccatcgctct atctacaacg tttacggcga tctgtttaag tattttgatg
     9301 ccctccagat tggcctgacg gccacgccga ttgacatggt gagtaaaacc actttcggcc
     9361 tgtttggttg tgaaggacgt attcctaccg ccaactacag cctggaagat gccatcgccg
     9421 ataacaatct ggttccctat gaggttgtca cgcacaccac agaattcctg cgcgaaggca
     9481 tcaaacggga gaaattaagc gacgcacaaa tccgcgagct ggaagaacaa ggcatagatc
     9541 ccaatacgct ggagtttgat ggcaaggcgc tggatgaggc gatctacaac aaagacacca
     9601 accgctatat cctgcgtaac ctgatggaaa atggcctgaa agatcgggac ggccagttgc
     9661 caggtaaaac catcattttc gcccgtaacc acaaacacgc gctgctattg aatgaattat
     9721 tcgacgatat gtacccgcag tttgccgggc gcttctgcca ggtcatcgac aattacgatc
     9781 cccgcgccga gcaactgatt gatgatttta aagggctgga tgaaagcacc aacaaagagc
     9841 tgaccatcgc tatctccgtt gacatgctcg ataccggtat tgatgtcccg gaaattgtga
     9901 atctggtctt tgccaaaccg gtcaaatcaa aagtgaagtt ctggcagatg attggccgtg
     9961 gtacgcgcct ctgtccgggg ttgtacggtt acgacgataa cggcaaacct ctggataaac
    10021 agaaattccg cattttcgat cactggggca attttgagta tcacgaactg cataccgaag
    10081 aggccgaagt cacagcgaca aaatcgcttg cacaaaaacg cttcgaagcg tgggttatgc
    10141 tgggggccgc tgcgcaacgt aaattcgaca aacaggcggt ggatttagtt gctcaccagc
    10201 tacgcgagca aatcaatgca ctggatgaaa agtcgattgc cgtccaggaa aagtggcatc
    10261 aaaaagcgca gtacagcgat gaaaaagtgc tgcgccagct ttccccgaaa acacagcagg
    10321 atttgctatc tgtcctggcc ccgctgatgc agtggctgga cgtgcgcggg caaagcgacg
    10381 ctatgcgctt tgatatggat attctggcgg cgcaaactgc ccgttatacc aacccggaag
    10441 agctggatgt gctctggccg gtcatcgtcg aaaaagtgga gcgcctgccg ccgcatttgg
    10501 cgcaggtcca acagcaagga ccgcggatca atcagcttcg tgatttaggc tggtggaagc
    10561 aggccagcct ggaagagctg gaagatatcc gcattcatct gcgcggcatc atgcacctga
    10621 tggaaaaaga cgcgacgccg aaatttggtt cgatacaggt ggatattacc gaagatgcga
    10681 acctgattca gacggaaaac cgcaaaacca acatccgctc gattgacttc aaactctatc
    10741 gccagcaggt gcagggagcg ctggagccgc tgttccagca aaatccggtg ctgaagaaaa
    10801 tccgcaacgg cgagcctgtg acgcaaagcg agctggatga actggctaaa ctggtgctga
    10861 tccagaaccc taacgttgat atccgggcac tgaaagagtt ctacccgcag gcgaccgcca
    10921 gcctggataa actattgcgt accatcatcg ggatggacag cgacgcggtg gaagtgcgct
    10981 ttgctcagtt cgccgctgat aacagcctga ccagccagca attgcgcttc ctgtcattgc
    11041 tgaaaaacca cattcgcgat tacggcacca ttgaaatgcg gcagctcttt gaacagcctt
    11101 ttacgcatat ccacaacgaa ggcgttaccg gtgtgttccc tgatatagag cagatcgccc
    11161 gcctacaaaa gatagtcgaa gagctgggtg ttgtgaccga cgcagcaacg gtataatacg
    11221 cacatgacaa gccccggcag cggggctttt gtatttaaaa caggatataa aagctaacaa
    11281 tgatcactgg tgacttaaaa agtaaaatcg acggcctgtg ggaagatttc tgggtgggtg
    11341 gtatcaccaa cccgctgacc gtaatcgaac agatcaccta tctaatgtat tcccggatgc
    11401 tggatactca ggaacaacgc gacgagaagc gtaaacaaat cgcgggtatt gattttaaac
    11461 cgcgttttgc accagaacag caggagttcc gtttcagtca ctatagcaat cttggctcgg
    11521 atgagatgat ggaagtggtg cgcgatggtg tattccagca ttttcgtcag ctcggccagg
    11581 ctgatgcgtc gaaggtaacg ctgctgggca actttatgaa agatgcccgt ctggagattg
    11641 ttaagccttc attgctgacc aaagcggttg aggtgatcaa aaacctgcca ttggatcgcg
    11701 gcgacaccaa aggcgacctt tacgaatacc tgttaagcaa gctgaccact gccggaatca
    11761 acggccagtt tcgcacaccg cgccacatta tccgcacgat ggttgaaatg atggagccga
    11821 accccgcccg cggcgagacg atttgcgatc cggcctgcgg caccggtggc tttctggcaa
    11881 ccagctatga atatctactg gagaagtaca gctcgctgga atccattcat accgagattg
    11941 gcaccaacga acgtggtgag ctggaagagc aaaaaatctt taccggcgat ctgttgaccc
    12001 cgtggcgtaa ccatgtggat aacaatatgt tccacggtta cgactttgac accacaatgt
    12061 tacgtatcgc cgccatgaac ctgattatgc acggcgtgga agcgccggat atccactatc
    12121 aggacaccat gagccagagc ttcagcacaa acttcccgca ggccagtaaa aacgctttca
    12181 acctgattct ggcaaacccg ccgtttaccg gttcactgga cgaagaagat atcgactcca
    12241 cgctgtcggc aatggtgaaa accaaaaaaa ccgaactact gttcctggcg cgtattctgc
    12301 aaatgctgaa agtgggcggg cgcagtgcca ctatcgtgcc gcagggcgtg ctgtttggct
    12361 ccagtaaggc gcaccagtca cttcgcaaaa cgctggtgga agataaccaa ctggaagcgg
    12421 tgatcaacct gccttccggc gtatttaaac cttacgctgg cgtggcgacg gcgatcctga
    12481 tctttaccaa aggcggtcag acggacgagg tctggttcta cgatctgcaa aatgatggct
    12541 acagcctgga tgacaagcgt aacccaatta aagacaacga cctgccgcat ctgctggcaa
    12601 gctggaagca ttaccgtact ttacgcgggc tgccggttga taactttatg ggtgagaagt
    12661 tagcctcgtt gctgaaacag cagtacccgg aagggattga tgctggcgtt gattttaaag
    12721 atcgcactca ggcggcgttt atagtgccaa aagaagatat tgctgcccag aaatacgatc
    12781 tctctattaa tcgttataaa gaagtggtgt atcaggctga ggagtatgaa gatccgaagg
    12841 tgatattgaa gcggttaaaa gatctggaaa aagagattct ggcggatctg gatgagctgg
    12901 aggggatgct gtgaaaaaaa atcaattggt cacacttggc aaacatatag acatattgtc
    12961 cgggtgtgca ttcccttcaa gtggattcaa taggaacaat ggtgttccac taatccgtat
    13021 tagagatatt ctttcaggaa aaaccgaaac t
//
LOCUS       DAJOTB010000131        13039 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJOTB010000131 DAJOTB010000000
VERSION     DAJOTB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.131"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            391..1080
                     /gene="mutH"
                     /locus_tag="OHN28_003742"
     CDS             391..1080
                     /gene="mutH"
                     /locus_tag="OHN28_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCQ2299052.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1149..1862
                     /locus_tag="OHN28_003743"
     CDS             1149..1862
                     /locus_tag="OHN28_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2299053.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2000..2218
                     /gene="ygdR"
                     /locus_tag="OHN28_003744"
     CDS             2000..2218
                     /gene="ygdR"
                     /locus_tag="OHN28_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCQ2299054.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2326..3366
                     /locus_tag="OHN28_003745"
     CDS             2326..3366
                     /locus_tag="OHN28_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCQ2299055.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3398..4591)
                     /gene="lplT"
                     /locus_tag="OHN28_003746"
     CDS             complement(3398..4591)
                     /gene="lplT"
                     /locus_tag="OHN28_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCQ2299056.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4584..6743)
                     /gene="aas"
                     /locus_tag="OHN28_003747"
     CDS             complement(4584..6743)
                     /gene="aas"
                     /locus_tag="OHN28_003747"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCQ2299057.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7329..8360
                     /gene="galR"
                     /locus_tag="OHN28_003748"
     CDS             7329..8360
                     /gene="galR"
                     /locus_tag="OHN28_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCQ2299058.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8367..9629)
                     /gene="lysA"
                     /locus_tag="OHN28_003749"
     CDS             complement(8367..9629)
                     /gene="lysA"
                     /locus_tag="OHN28_003749"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCQ2299059.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9751..10686
                     /gene="lysR"
                     /locus_tag="OHN28_003750"
     CDS             9751..10686
                     /gene="lysR"
                     /locus_tag="OHN28_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCQ2299060.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10673..11365)
                     /gene="ygeA"
                     /locus_tag="OHN28_003751"
     CDS             complement(10673..11365)
                     /gene="ygeA"
                     /locus_tag="OHN28_003751"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCQ2299061.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11494..12912)
                     /gene="araE"
                     /locus_tag="OHN28_003752"
     CDS             complement(11494..12912)
                     /gene="araE"
                     /locus_tag="OHN28_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCQ2299062.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3314 a         3509 c         3204 g         3012 t
ORIGIN      
        1 ataaaaaaga gtaattcgtg acccaggtca cacctctcat ttacgggttg gcgaaagatc
       61 aaccaacgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa ccactaaaaa
      121 ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag tagtgaaggt ttttcacagt
      181 tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag tgaccagatt
      241 tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc aacctaaaaa gcaatataaa
      301 tcagagaatt gaacaacgca tgtggaaaaa gttacactgc gaatattcgg cacataattg
      361 ctgtttgttt tttaaccaag gtatcatgac atgtcccaac ctcgcccact gctctctcct
      421 cccgaaactg aagaacaatt gttagcgcaa gcacaacaac tttctggtta tacattggga
      481 gaactggcgg cacttgccgg gctggttacg ccggagaatt taaaacgcga taagggctgg
      541 attggcgtgt tactggagat ctggctaggt gccagcgcag ggagtaaacc tgagcaagat
      601 tttgctgctc tgggcgtgga acttaaaact atccctgtgg atagtcttgg tcgtccgctg
      661 gaaacaacat tcgtttgtgt tgccccgtta acgggcaata gcggggtgac atgggaaacc
      721 agccacgtgc gccacaagct caaacgcgtg ctgtggatac cggttgaagg cgagcgcagc
      781 atcccgctgg caaagcgtcg cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa
      841 gaccggcagc tacgcgaaga ctgggaagaa ttaatggata tgattgttct cggtcagatt
      901 gagcggatca ccgctcgtca cggggaatat ttacagatac gaccgaaagc agcgaatgcg
      961 aaagcgctga ccgaagctat tggtgcccgg ggcgaacgga ttctgacgct gccgcgcgga
     1021 ttttatttga agaagaattt caccagtgcg ctactggccc gtcattttct gatccagtag
     1081 ccatcgcttt gacctgccgc tttccgggca tataattacc gcttcatttt tttggcaggg
     1141 ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg ctcggtacgc
     1201 tgacgctgct ggagatcgtt cttgggatcg acaatattat tttcctttct ctggtggtgg
     1261 caaagctccc aacagcacaa cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg
     1321 tcatgcgtct ggcgctgctg gcatccatcg cctgggttac gcgcctgacg aatccgcttt
     1381 ttacaatatt cagtcaggaa atttccgccc gtgatttgat tctgcttctg ggtggcttgt
     1441 tccttatctg gaaagccagc aaggaaatcc acgaatccat cgaaggtgaa gaagaagggc
     1501 tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca gattatgctg ctggatatta
     1561 tcttcagcct cgactcggtg attaccgctg tgggtctgtc agatcacctg tttattatga
     1621 tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt
     1681 ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct
     1741 ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc tacttcgcga
     1801 tgttcttctc tattgcggtt gaaagcctaa acctgattcg caacaaaaag aatccgctct
     1861 gatactccgt acgctctcct gcgggagggc gtactcactc ctgttaccct cacagtttaa
     1921 gattttcctg ctttcaagat atataacgtc ggtttataaa cagactatta tcataggtgg
     1981 gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag tcggactggc
     2041 gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg gccgtatgat
     2101 tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt atcacgatca
     2161 gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca
     2221 aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg
     2281 ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt
     2341 ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa
     2401 cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac
     2461 cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaactca agggctaacc
     2521 gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc
     2581 tccaaagtga gcggaccgtc gcgcaataat gacaaaggca tccgcccgga tcaggcgctg
     2641 gatcggaaga atatccgtga agcgctgcat gacagcctca agcgtctgca gactgattac
     2701 ctcgatctct atcaggtgca ctggccgcag cgcccaacca actgcttcgg caaactcggt
     2761 tatagctgga cagattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca
     2821 gagtaccaac gcgcgggaaa aattcgttat ataggcgtgt cgaacgaaac tgcatttggc
     2881 gtaatgcgct acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag
     2941 aacccctaca gcctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat
     3001 gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat
     3061 ctcaatggtg caaaacccgc tggcgcacgt aatacgctct ttagtcgctt cacccgctat
     3121 agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatagc
     3181 ctggaccctg ctcagatggc gctcgcattt gtacgccgtc aaccgtttgt tgccagcact
     3241 ctgctgggcg caaccacgat ggagcagctg aaaactaacg tcgaaagttt gcatctggag
     3301 ttgagcgaag acgtattagc tgaaattgaa gcggtgcatc aggtttatac gtatcctgca
     3361 ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc
     3421 acagcgccgt tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg
     3481 ggatgcctac cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt
     3541 cgccaaggtt ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct
     3601 gtagcaacgc attgagcgga acgacaaaaa aaccccccag cacgccaatc agcatcaaca
     3661 aagcataggc tggcagcagc tcgtgttgca gggagaaaat cagtaccacc acgccaatca
     3721 aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac
     3781 ctgcaccaac cacgatacca atcgctacca tcgcgttgag ataggtcggc gtagcgttat
     3841 cggtaatacc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg
     3901 ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg
     3961 aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag attccaggac tgccccggac
     4021 gcgccgccgc cagtttggga atgtagatat tggcaatgac cgcgccaccg taggccagtg
     4081 cgcatgcggc cagggcgatc aggacatgcc agtcagccag aacaccacca gctacggaac
     4141 cgagcaaaat cgccgctatg gtagacgcct ccattaaacc gttagctttt actaacttac
     4201 tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa
     4261 cacccaccaa cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca
     4321 gtagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct
     4381 gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct
     4441 ggctccactc cggatagaac tgcgctttca gtaacgccag agtagcaaac agtagcgcat
     4501 tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca
     4561 acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt
     4621 ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga
     4681 atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgtaactta
     4741 tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg
     4801 ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc
     4861 atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc
     4921 tgcacaaagc cctgctcatc aaaacgcacg atatcgccag tgtcatacca gccgcgctcc
     4981 atttcgccgc gaatattctc ggcggtgggc acttccagta caccaggctt ctccacccgc
     5041 agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca
     5101 ggaaccgaca acaggcgcgc atccattcct ggtagaatac gccctaccgt accgggtttc
     5161 gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacacc gtagccttca
     5221 aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct
     5281 gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcaaa gcgcgcgtag
     5341 tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaactcc
     5401 ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtgagcagt
     5461 ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc cgacataaag
     5521 cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct caacgttcgc cagaatgctt
     5581 ttatggctat ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa gaggatcagc
     5641 gcttcttctt ccggctgctg ttttaactgt gccagacgcg gcatcagcaa atgagcgaaa
     5701 atccacactt tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc
     5761 acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctgacgggaa
     5821 gtgaagatgg ttttgatttc agccgccgta atagcactgg tcagcccttt taccccggcg
     5881 gtgtagttca tcattgcggg aatgcggcga cgggcgatgg ccccaaaaat cactgccgca
     5941 ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca
     6001 aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag
     6061 ttgacgtctt cgacacattt cttcccggct ccgaagcggt acattgcgct cagtaaagat
     6121 tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgcct ccatcattat ttgatgcagc
     6181 atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat ctccacctgc
     6241 gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc
     6301 aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca
     6361 gacttcgccg cgacaaatcc cgcgccatcg tagattttca tcagcgagcc agtggtggtg
     6421 atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt
     6481 ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa
     6541 cgcatatacc actgttggct tattgaggtg taaacggcaa ataccggacg tacgggtaaa
     6601 aacaatgcca gcagtatgcc atcaataaaa gagacgtgat taggcgtgat tagaacgcgc
     6661 tcgcccttca gtgccttggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa
     6721 tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg
     6781 gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc
     6841 tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca
     6901 ccaatcaata cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa
     6961 aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga
     7021 ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca
     7081 acccgaagtt gacttcacct atcaatacct ctgggattac cactttagca acctgaagcc
     7141 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
     7201 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacaatgaa aacacgccgc
     7261 cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag
     7321 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
     7381 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccgactg gctgtgcata
     7441 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
     7501 ccactgaaac gatcggtctg gtcgttggtg atgtttccga tccgtttttc ggtgcaatgg
     7561 tgaaagcggt cgaacaggtg gcttatcaca ctggtaattt tttattgatt ggcaacggtt
     7621 accacaacga acaaaaagag cgtcaggcca ttgagcaact gatccgccat cgctgtgctg
     7681 cgctggttgt ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa
     7741 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
     7801 tggacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
     7861 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
     7921 attacgatgc ccttgctgaa agtggtattc cggccaatga ccggctggtg acatttggcg
     7981 aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggcagaaatt
     8041 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gtgctcaatg
     8101 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
     8161 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgacgc
     8221 aggctgccga actggctttg gcgctggcgg ataatcgccc tctcccggaa atcactaatg
     8281 tctttagtcc gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg gaggcaagtc
     8341 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcaatg
     8401 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
     8461 ggacggctgt tgtagttgga tgacattgac gcgccatatg cccctgtatc atgcagtacc
     8521 agataatcac ccgccttcac ttccggcaag gcgcgggttt cgacatttcc cccttcctgc
     8581 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
     8641 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
     8701 attgccgggc gcatcagatc gttgaacccg gcatcaacca gaacaaagtg gcggctaccc
     8761 atttgtttga cgctccgcac ttgagtaatt aatacgccag cctgcgctac cagaaagcga
     8821 cccggttcaa tttccagttt cacaggatgg cccaaatggc gggcgatttg ctcacgcgcg
     8881 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
     8941 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gaggacttga
     9001 cgcaccatag caccacatac ctgttccaga tgggcataat cgacgccaga accaatgtgc
     9061 atgtgaatgc cgaccagctg tagatgatga cgttgtatca cgtccagtgc ggcgggcaga
     9121 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgc
     9181 ccgtgaccaa accccggatt aacacgcagc catacccgat gccctggcga aacctgcccc
     9241 agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
     9301 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcagg gtgcgtttgc
     9361 ggattgtaac ccgccgccag tgcacgctct atttcaccta acgagacgga atccactttt
     9421 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
     9481 cgcaccacat caaactgttt cagcgccgca atctgccgac gaataatttg cgcatcgtag
     9541 acccacaccg ggcagccaaa ttctgcgggc aaacgcagca gattttcggc ggtgagatcg
     9601 gtatcagtgc tgaacagtga atgtggcata acaaactcca gataagtgct tttttatgat
     9661 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga
     9721 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
     9781 atttttcatg cggtaatgac cgccggaagc ctgactgagg cggcacacct gctacacacc
     9841 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
     9901 tttgagcgca tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
     9961 gtgcaacgat cctggtacgg actggatcgc attgtcagtg ccgcagaaag tctgcgcgag
    10021 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    10081 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    10141 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgaa
    10201 acgctccata cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    10261 gtgttacctc cgggccatcc gctggcggta aaaaaggtat taacgccgga tgattttcac
    10321 ggtgagaact acatcagcct ttcccgtact gacagctatc gccagttgct ggatcaattg
    10381 tttactgaga atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    10441 tgcgcaatgg tacgggcggg ggtaggcgtt tcagtggtta acccgctcac cgcactggat
    10501 tatgcggcaa gcggtttagt ggtgcggcgg ttcagcattg cggttccatt caccgtcagc
    10561 ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta
    10621 caagcgggat taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
    10681 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcagaacac
    10741 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    10801 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    10861 gccccagaca cagttcttca aaaataatct gattaatttt cgcccggtca tccgcttcag
    10921 gaataagaca gttgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    10981 tggtgtaacg tgtacccagc agcgccacac gagtcattcc tgccccggta attgcacgtc
    11041 cagtggcatc cgctatgtgt aagaaaggca gagagcaacg agactcaatg gcatccgcca
    11101 ctttgtgcat cgtattggtg cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    11161 gcgccgcctc agccagaata tccccggttt tgtcccactc accgcgacgc tggcactctt
    11221 ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc
    11281 gctgtttaat gccttcattt atcagacgat agtaaggaat agtggattcc cagctcattc
    11341 ctcccagcaa accaattgtt ttcattcctc tctcctgatg agtgttaccg caacaaggct
    11401 gaataaggaa cgaggcaaac gcctcaacgg ccaggtgcct aatctctatt aacgaaaaaa
    11461 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttttc
    11521 gcctgccatc agtttgcgct cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    11581 ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc
    11641 agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca
    11701 gtttgtcgtc gttgagcagg tgatgccaaa atcgcggcat ttcagcggct gaatttcaga
    11761 gcacaagatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    11821 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    11881 gcagtaaccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagcgccgg
    11941 cttacgccct gctttatcta ccgtaaagac cgcaatgaag gtagcgaaca taaaggtcag
    12001 ccccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    12061 gatacgtggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    12121 caacataccg agaaatacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
    12181 gccctgtttt aacttcaggc tttcgcgaat ttcgttgagt tcttctcgcg ctttttccga
    12241 cgtatcgcgc agcatacgca atacttcttc cgcctcaata tgacgcccct tttccgccag
    12301 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
    12361 aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    12421 cgccagcacg atgccgagag tgaccatcaa ctggtacatg ctgatcatct taccgcgaac
    12481 gttttcactt gccatttcag aaagatatag aggagcggtg taagacgcga tcccgacagc
    12541 aatgcccaac accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc
    12601 tatcgaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg
    12661 gaacgacagc caaccattaa acagcgcacc aattgctgca ccgagcatca tgctactgac
    12721 cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc
    12781 ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac
    12841 aaacatattc atacgccgcg tatcccgcag aggacgtggc gttaaagcag attccgtatt
    12901 gatagtaacc attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca
    12961 attcacacag gtgtcaggtc ggaaatagca taaatatgga ttaaattgct acgacatgtc
    13021 gttatgtgat ggatattcc
//
LOCUS       DAJOTB010000132        13007 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJOTB010000132 DAJOTB010000000
VERSION     DAJOTB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.132"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            119..949
                     /locus_tag="OHN28_003753"
     CDS             119..949
                     /locus_tag="OHN28_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2299063.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            981..1916
                     /locus_tag="OHN28_003754"
     CDS             981..1916
                     /locus_tag="OHN28_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2299064.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2009..2251
                     /locus_tag="OHN28_003755"
     CDS             2009..2251
                     /locus_tag="OHN28_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCQ2299065.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            2241..3692
                     /gene="panF"
                     /locus_tag="OHN28_003756"
     CDS             2241..3692
                     /gene="panF"
                     /locus_tag="OHN28_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCQ2299066.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3704..4585
                     /gene="prmA"
                     /locus_tag="OHN28_003757"
     CDS             3704..4585
                     /gene="prmA"
                     /locus_tag="OHN28_003757"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCQ2299067.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4914..5879
                     /gene="dusB"
                     /locus_tag="OHN28_003758"
     CDS             4914..5879
                     /gene="dusB"
                     /locus_tag="OHN28_003758"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCQ2299068.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5905..6201
                     /gene="fis"
                     /locus_tag="OHN28_003759"
     CDS             5905..6201
                     /gene="fis"
                     /locus_tag="OHN28_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCQ2299069.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6286..7170
                     /gene="yhdJ"
                     /locus_tag="OHN28_003760"
     CDS             6286..7170
                     /gene="yhdJ"
                     /locus_tag="OHN28_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2299070.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7254..7433
                     /locus_tag="OHN28_003761"
     CDS             7254..7433
                     /locus_tag="OHN28_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCQ2299071.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7436..8098)
                     /gene="acrS"
                     /locus_tag="OHN28_003762"
     CDS             complement(7436..8098)
                     /gene="acrS"
                     /locus_tag="OHN28_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCQ2299072.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            8497..9654
                     /gene="acrE"
                     /locus_tag="OHN28_003763"
     CDS             8497..9654
                     /gene="acrE"
                     /locus_tag="OHN28_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCQ2299073.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9666..12770
                     /gene="acrF"
                     /locus_tag="OHN28_003764"
     CDS             9666..12770
                     /gene="acrF"
                     /locus_tag="OHN28_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCQ2299074.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         3215 a         3120 c         3285 g         3387 t
ORIGIN      
        1 attaagatat tttagctgca attttgaccc atgattttct tttcgaattg tcgatatgat
       61 tgaaggataa ctttcaaaga aaaatacaag gggctatagt gttacgcatt aaaggccaat
      121 gaagagtgtt aacgtgccga gaaaatcgtc taacttactg aaacgtagat taaaaattgc
      181 agaaattgtt gccgcccagg gtgaaatcaa agttgatgac ttatctgcgc aacttggtgt
      241 ctctggcgtg acgatccgcg gcgatctcag ttatctggag caacagggat atttaaagcg
      301 atcatttggc ggtgctattg cgacaccact ctcctctgaa ccagaagcga tacaaccgat
      361 cgcgccattg tctctggcac aacaaatgga aatagcacga cattgcgcac ggttaattcg
      421 cgatcgtgac acgctatttc ttggacatgg gacaatctgc cgtaagatca ttccattgct
      481 cagcgaagtg aaaaaactgc gtctgatcac caacgatccc gaacatgcgc tgctggcaaa
      541 tcagtttatt gatggcgaaa taatactggc tggcagtgaa atgctgcgcc ccgacaccgc
      601 actggccgga aaacagcttg aacaggcact tcagcatttc accattacac atagcattct
      661 ggaaatcagc catctggatg ctgatggtac tttaaatatc aacgttccca gactcgccgc
      721 tgcctggcag ctctgtttcg atcatgccca gaataagacg gttatcgttg ccgccgatcc
      781 tgcgccatca acggctgcta tcgggcattt gcatcaggca gaaaatgtga tcgtcagccg
      841 cagcattaac gaacaatatc agcaacaatt gcagtcggct gatttcgtta tcctttatac
      901 cagtaatgag tgctttacct ggtccaatcg cgaaaggctt caggaatgag aggggttttg
      961 tgacaataac tatctgcgct atgggcgaat tgctggccga gtttttgtcc cgcaacccac
     1021 atcaaaaatt cactcagcct ggggagttta tcgggccatt tcccagcggt gcgccagcaa
     1081 tttttgctgc tcaggtggca aaactgtccc atcgggccat cttctttgga tgtgttggta
     1141 atgatgattt tgcccgactc attatagagc gtctccgtca tgaaggtgtc attaccgatg
     1201 ggatccatgt tatgaacaat gccgtcacag gtacggcgtt cgtgagttat caaaatcccc
     1261 agctgcggga tttcgtcttt aatatcccta acagcgcctg cggtttgttt actgccgagc
     1321 acattgataa ggatctgctt aaacagtgta accatctgca tattgtgggc tcatcgttgt
     1381 tctcatttcg catgatcgat gtcatgcgta aagcaataac gacgatcaaa tcggctggcg
     1441 gcaccgtttc tttcgatccc aatattcgca aagagatgct gagcattcct gaaatggcgc
     1501 aggctctcga ttatttgatt gaatatacgg atatttttat ccccagcgaa agcgaactcc
     1561 ctttcttcgc gcgtcacaaa aatctgtcag aggaacagat tgttagcgat cttctccacg
     1621 gcggcgtaaa acatgtggcg ataaaacgcg cccagcgtgg ggctagctat tacaagctta
     1681 aaaacggtac attacacgcc cagcatgttg caggtcacga tatcgaaatt atcgatccaa
     1741 cgggtgcagg cgactgcttt ggcgcaacgt ttatcactct tttcttatcc ggtttcccgg
     1801 cacacaaggc gctgcaatat gcaaatgcca gcggcgcgct cgccgtaatg cggcaaggtc
     1861 cgatggaagg gatatcctca ctggcagaca ttgaagactt tttgcagcag cactgaacgg
     1921 caattttctg tcagattatt ttccctgcct gaagacttgc cgtagaattg cccgtttctc
     1981 gtatcccccc atgaggagga ccccttccgt ggacactcgt tttgttcagg cccataaaga
     2041 ggcgcgctgg gcgctggggc tgaccctttt gtatctggcg gtatggttag tagccgctta
     2101 cttacctggc gttgcccccg gttttaccgg ttttccgcgc tggtttgaga tggcctgcat
     2161 cctgacgccg ctgctgttta ttggactgtg ctgggcgatg gtgagattta tctatcgcga
     2221 tatcccactg gaggatgacg atgcagcttg aagtaattct accgctggtc gcctatctgg
     2281 tggtggtgtt cggtatctct gtttatgcga tgcgtaaacg gagcactggc accttcctta
     2341 atgagtattt cctcggcagc cgctctatgg gcggtattgt gctggcgatg acgctcaccg
     2401 cgacctatat cagtgccagt tcgtttatcg gcgggcctgg agctgcttat aaatacggcc
     2461 tgggctgggt attgctggcg atgattcagc ttcctgcagt ctggctttcg ctcggtattc
     2521 tcggcaagaa gttcgcaatt cttgcgcgcc gctacaatgc ggtgacgctg aacgatatgc
     2581 tgtttgcccg ctaccagagt cgtcttctgg tgtggctggc gagtttgagt ttgctggttg
     2641 cgttcgttgg tgcgatgacc gtgcagttta tcggcggtgc gcgcctgctg gaaaccgcgg
     2701 cgggtattcc ttatgaaacc gggctgctga ttttcggtat cagcattgcg ttgtataccg
     2761 cctttggcgg ctttcgcgcc agcgtgctga acgacaccat gcaagggctg gtgatgctga
     2821 ttggcaccgt tgtgctgctt attggcgtgg tacatgccgc aggtggttta agtaatgcgg
     2881 tagacacgct gcaaaccatc gatccgcaac tggttacgcc acaaggcgct gacgatattc
     2941 tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg ctttggcgtt ataggcctgc
     3001 cgcatactgc ggtgcgctgt atctcttata aagacagcaa agcagtacat cgggggatca
     3061 tcatcggtac gattgtagtc gcaatcctga tgttcggtat gcatctggcc ggagcgttag
     3121 gtcgggcggt gatccccgat ctcaccgtac cggatctggt gatcccgacg ttaatggtaa
     3181 aagtgctgcc accgtttgcc gccgggatct tcctggctgc accgatggct gcgatcatgt
     3241 cgacaattaa cgcccaactg ctgcaaagtt ccgctacgat cattaaagat ctctatctga
     3301 atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa gcggatgtcg gcggtgatca
     3361 cgttggttct cggtgcgttg ctgctgcttg ccgcctggaa gccgccagaa atgatcatct
     3421 ggctgaattt gctggccttc ggtgggctgg aagccgtttt cctgtggccg ctggtgctgg
     3481 gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag tgcgatgatc gttggcggcg
     3541 tgctgtatgc cgtactcgcg aagctgaata ttcagtacct gggcttccac cctatcgtgc
     3601 cctcgttact actaagtttg ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc
     3661 cgcaagctac cgttttgact actgataaat aaagagtttt gccatgcctt ggatccaact
     3721 gaaactgaac accaccggcg caaacgcgga agatcttagc gatgcgctga tggaagcggg
     3781 tgccgtttct atcacttttc aggataccca cgatacgcca gtatttgagc cgctgccggg
     3841 cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg ttcgacgctg aaaccgatat
     3901 gaacgacgtg gtggcgattc tggaaaacca tccgctgctc ggcgcgggct tcgcgcataa
     3961 aatcgaacaa ctggaagata aagactggga gcgcgaatgg atggataatt tccacccgat
     4021 gcgctttggt gaacgactgt ggatctgccc aagctggcgt gatgtgccgg acgaaaacgc
     4081 cgtcaacgtg atgttagacc cagggctggc gtttggtacg ggtacccatc caactacctc
     4141 tctgtgcctg caatggctcg acagcctcga tttaaccggt aaaacagtca tcgactttgg
     4201 ctgcggttcc ggcattctgg ctatcgcggc gctgaaactg ggtgcagcaa aagccattgg
     4261 tattgatatc gatccgcagg cgatccaggc cagccgcgat aacgccgaac gtaatggcgt
     4321 ttctgatcgt ctggaactct acttaccgaa agatcagcca gaagaaatga aagccgacgt
     4381 ggtggtcgct aacatccttg caggcccatt acgtgaactg gcaccgttaa tcagcgtcct
     4441 gccagtttca ggcggtttgc tgggcctttc cggtattctg gcaagccagg cagagagcgt
     4501 ttgcgaagct tatgccgaca gcttcgctct ggacccggtc gtggaaaaag aagagtggtg
     4561 ccgcattacc ggtcgtaaga attaaccttc gcatcgccgt atggcgacgc agagcgaagt
     4621 gcgagcgatg tcacaaaagg cacgttaatt tgccgattat ttacgcaaat ttgcgtgcca
     4681 aaattttcat tcataaagaa aaattgagaa cttactcaaa tttctttgag tgtaaatttt
     4741 agtcactatt ttctaatatg atgattttta tgagtaatta tcgcgctacg ctcattttaa
     4801 atgcaattct ttgatccatc tcagaggatt ggtcaaagtt tggcctttca tctcgtgcaa
     4861 aaaatgcgta atatacgccg ccttgcagtc acagtatggt catttcttaa ctcatgcgca
     4921 tcggacaata tcagctcaga aatcgcctga tcgcagcgcc catggctggc attacagaca
     4981 gaccttttcg gacgttgtgc tacgagatgg gagccggatt gacagtatcc gagatgatgt
     5041 cttctaaccc acaggtttgg gaaagcgaca aatctcgttt acggatggtg cacattgatg
     5101 aacccggtat tcgcaccgtg caaattgctg gtagcgatcc gaaagaaatg gcagatgcag
     5161 cacgtattaa cgtggaaagc ggtgcccaga ttattgatat caatatgggt tgcccggcta
     5221 aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca gtacccggat gtcgttaaat
     5281 cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt taccctgaaa attcgcactg
     5341 gctgggcacc ggaacaccgt aactgcgaag agattgccca actggctgaa gactgtggca
     5401 ttcaggctct gaccattcat ggccgtacac gcgcctgttt gttcaatgga gaagctgagt
     5461 acgacagtat tcgggcagtt aagcagaaag tttccattcc ggttatcgcg aatggtgaca
     5521 ttactgaccc gcttaaagcc agagctgtgc tcgactatac aggggcggat gccctgatga
     5581 taggccgcgc agctcaggga agaccctgga tctttcggga aatccagcat tatctggaca
     5641 ctggggagtt gctgcccccg ctgcctttgg cagaggttaa gcgcttgctt tgcgcgcacg
     5701 ttcgggaact gcatgacttt tatggtccgg caaaagggta ccgaattgca cgtaaacacg
     5761 tttcctggta tctccaggaa cacgctccaa atgaccagtt tcggcgcaca ttcaacgcca
     5821 ttgaggatgc cagcgaacag ctggaggcgt tggaggcata cttcgaaaat tttgcgtaaa
     5881 cagaaataaa gagctgacag aactatgttc gaacaacgcg taaattctga cgtactgacc
     5941 gtttctaccg ttaactctca ggatcaggta acccaaaaac ccctgcgtga ctcggttaaa
     6001 caggcactga agaactattt tgctcaactg aatggtcagg atgtgaatga cctctatgag
     6061 ctggtactgg ctgaagtaga acagcccctg ttggacatgg tgatgcaata cacccgtggt
     6121 aaccagaccc gtgctgctct gatgatgggc atcaaccgtg gtacgctgcg taaaaaattg
     6181 aaaaaatacg gcatgaacta attcaggtta gctaattgct tgattaaaag gcgctactcg
     6241 gcatggggaa gcgccttttt tataggtgtc acaaagggag tgaccatgag aacaggatgt
     6301 gaaccgaccc gatttggtaa tgaagcaaag accatcatcc agggtgatgc attaaccgaa
     6361 cttaaaaagc tccccgccga aagtgtcgat ctgatctttg ccgacccacc gtataacatc
     6421 ggtaaaaatt ttgatggtct gatcgaagcc tggaaagaag atctgtttat cgactggctg
     6481 tttgaagtga ttgcagagtg ccatcgcgtt ctgaaaaagc agggcagcat gtacatcatg
     6541 aatagcacgg aaaacatgcc ctttatcgat ctccagtgcc gcaagctttt taccatcaaa
     6601 agtcgcatcg tctggtcata tgacagttct ggagtgcagg cgaaaaaaca ctacggctcc
     6661 atgtacgaac ccatcctgat gatggtgaaa gacgcaaaga actacacgtt caacggtgat
     6721 gctattctgg tagaagccaa aaccggatcg cagcgcgcgt tgatcaatta tcgcaaaaat
     6781 cctccacagc cttacaatca tcaaaaagta ccgggtaacg tctgggattt tccgcgcgtg
     6841 cgttatttga tggatgaata tgaaaaccac ccgacgcaaa aaccggaagc cttgctgaaa
     6901 cgcattattc tcgcctcttc caacccaggc gatatcgttc tcgacccgtt tgccggtagc
     6961 tttactaccg gtgccgtagc cgtcgccagc gggcgaaaat tcattggtat tgagatcaac
     7021 agcgagtaca tcaaaatggg gcttcgacgg ctggatgtcg cgtcgcatta ctctgcggaa
     7081 gaactggcga aagtgaaaaa aagaaagacc ggcaacctgt caaaacgaag ccggttaagc
     7141 gaagttgacc ccgatctcat tgcaaagtaa aggaagtgta agcctggttt tcagattatt
     7201 catttcgtgt atatttcctg ccagacttgg ttaaacatgc acaggctcag gtaatgattc
     7261 gcaagtattg gtggctcgtc gttttcgctg tcttcgtttt tctgtttgat actttactga
     7321 tgcagtggat tgaactgctg gcaacagaaa cagacaaatg ccgcaatatg aactcagtta
     7381 atccactaaa actggttaac tgtgacgaac tgaattttca ggacagaatg tgaatttata
     7441 tgacacttaa ttcattcgtt ttatgaatta atttcgttat gttttcatct ggcatgaaca
     7501 ttcttaatac gttatcgacc agagcggggg cttgtttata aagatcataa cccgccatat
     7561 tcattaacca gttttgaaca attccgctga aggcaccatc aataataatc atcacaacat
     7621 ctaaatcgag attatttgct acacaacctt gttgctgaca cgcctgcaat acttcgcgga
     7681 gagtctgcgg attaaagccc atcttttcgc gtatcactcc ctcggccagc atctcatcat
     7741 taaattcaca tttgtgatat aagattttca gcaacgcctg ctggcgggga attttggcaa
     7801 tatattgcaa accgacaatc aatttttcac gcaattgctg aaacgggtca tgctctaacc
     7861 cagcggtcaa gtgatcctgg attaactccc gcaatgaagg ctgttgcaac cacatctcat
     7921 taaacagttg agtcttgttt tcgaagtgcc agtagatagc gccacgcgta acgttagcag
     7981 cgtcggcaat gtcgttgagc gtcgtcttgc ttacgccatg ctgcgcaaac tgggcgatgg
     8041 cagtttcaat cagttcttgc cgggtcttca gggcttcagc tttggttctt tttgccatga
     8101 ttaattattc aggaaataaa tatattcgac acaggatgag aaaatagcga aggttaatct
     8161 atcacctaat gtgtatttat acgagaggct aatattgcgt tgctataaat cgtcaaataa
     8221 atgatatgta ttatttacat aaaatacatt cacttgacct atacatattt caatttacga
     8281 gattttaatt ctatttcttt cattccgcgt caaaacaaaa cagtagaata ttaatctttt
     8341 tttgtgttta tgtgccttga gttgccagta ttcataacta ttccttacat cgacgaatga
     8401 taatttgtag gatagcgaac tgtatttttc tttctgcgag ttaacgcatt gcctttttgg
     8461 gcaaataacg cgcttttggt tttttgagga atagtaatga cgaaacatgc caggtttttc
     8521 ctcctgccct cctttattct gatctccgcg gctttaatcg ccggttgtaa cgataaggga
     8581 gaagagaaag ctcacgtcgg tgaaccgcag gttaccgttc atattgtgaa aacggccccg
     8641 ttagaagtta agactgaatt accaggccgc accaatgctt accgtatagc cgaagttcgc
     8701 ccacaggtta gcgggatcgt actgaatcgc aatttcactg aaggcagcga tgtgcaagca
     8761 ggccagtccc tgtaccagat cgatcccgcg acctatcagg caagttatga cagcgcgaaa
     8821 ggcgaactgg cgaaaagtga agccgccgcc gccatcgcgc atttgacggt aaaacgttac
     8881 gttccgctcg tgggtacgaa atacatcagt cagcaggagt acgaccaggc cattgctgat
     8941 gctcgccagg ccgatgccgc cgtgattgcc gcaaaagcca cagtcgaaag cgctcgcatc
     9001 aatctcgctt ataccaaagt gactgcgcca attagcggac gtatcggcaa atcgaccgtg
     9061 accgaaggcg ctcttgtcac taatgggcaa acgactgaac tggcgactgt ccagcagctc
     9121 gatcctatct acgttgatgt gacccaatcc agcaacgatt ttatgaggct gaagcaatcc
     9181 gtagagcaag gaaatttgca taaggaaaac gccaccagca acgtagagtt ggtcatggag
     9241 aacggtcaaa cctatcccct gaaaggtacg ctgcaattct ccgatgtgac cgttgatgaa
     9301 agcaccggct ccataaccct acgtgctgtc ttccctaacc cgcaacatac gcttttgccg
     9361 ggtatgtttg tgcgcgcacg gattgatgaa ggcgtccaac ctgacgccat tcttatcccg
     9421 caacaaggcg ttagccgcac accacgtggt gatgcaaccg tgctgattgt taacaataaa
     9481 agtcaggttg aagcgcgccc tgtcgttgcc agtcaggcga ttggcgataa atggttgatt
     9541 agcgaaggtc tgaaatctgg cgatcaagtc attgtcagcg gcctgcaaaa agcgcgtccg
     9601 ggagagcagg ttaaagccac taccgatacc cccgcagata ctgcatcgaa gtaaggcaat
     9661 ctgacatggc aaactttttt attcgacgac cgatatttgc atgggtgctg gccattattc
     9721 tgatgatggc gggcgcactg gcgatcctac aattgcccgt cgcccagtat ccaaccattg
     9781 cgccgcctgc ggtttctgtt tcggcaaact atccgggtgc tgatgcgcag accgtgcagg
     9841 atacagtgac gcaggttatc gaacagaata tgaacggtat cgataacctg atgtatatgt
     9901 cctccaccag cgattccgcc ggtagcgtga caattaccct taccttccag tccgggaccg
     9961 atcctgatat cgcgcaagta caggtgcaga acaaactcca gctcgccacg ccgttgctgc
    10021 cgcaggaggt tcagcagcag gggatcagtg ttgaaaagtc cagtagcagc tatttgatgg
    10081 tggcgggctt tgtctctgat aaccctggca ccacacagga cgatatctcg gactatgtgg
    10141 cctctaacgt taaagatacg cttagccgtc tgaatggcgt cggtgacgta cagcttttcg
    10201 gcgcacagta tgcgatgcgt atctggctgg acgccgatct gctaaacaaa tataaactga
    10261 caccggttga tgtgattaac cagttgaagg tacagaacga tcagatcgct gccggacagt
    10321 tgggcggaac gccagcgtta ccagggcaac agttgaacgc ctcgattatt gctcagacgc
    10381 ggttaaaaaa tccggaagaa ttcggcaaag tgaccctgcg cgtaaacagt gacggctcgg
    10441 tggtgcgtct gaaagatgtc gcacgggttg aacttggcgg tgaaaactat aacgttatcg
    10501 ctcgcatcaa cggaaaaccg gcggcgggcc tggggattaa gctggcaacc ggcgcgaatg
    10561 ctctcgatac cgcgaaagcc attaaggcaa aactggcgga attacagcca ttcttcccgc
    10621 agggaatgaa ggttctttac ccttatgaca ccacgccttt cgtccagctt tctatccagg
    10681 aagtggtgaa aacgctgttc gaagccatta tgctggtgtt cctggtgatg tatctgttct
    10741 tgcagaacat gcgggcaaca ttgataccca ccattgctgt acctgtcgtg ttgttaggga
    10801 catttgccat actcgccgct tttggttact ccatcaatac gctaacgatg tttgggatgg
    10861 tacttgccat cggacttctt gttgatgatg cgatagtcgt tgtggagaac gtcgagcgcg
    10921 taatgatgga ggataaactc ccccccagag aagcgacgga aaaatcgatg tcgcaaatcc
    10981 agggcgcgct ggtaggtatc gcgatggtac tgtcggcggt atttattccg atggcattct
    11041 tcggtggctc aacaggggca atttatcgcc agttctctat cactattgtt tcagcaatgg
    11101 cgctttcagt actggttgcg ctgattctca cccccgcgtt atgtgcaaca ttgctgaaac
    11161 cagtctctgc cgagcatcac gaaaataagg gaggattctt cggctggttt aacaccactt
    11221 tcgatcatag cgttaatcac tacaccaata gcgttggcaa aatcctcggt tccacagggc
    11281 gatatttact gatctatgcg ctgattgttg caggaatggt ggtgttgttt ttacgtcttc
    11341 cgtcttcctt cttacctgaa gaggatcagg gtgtctttct gaccatgatt cagttacccg
    11401 ctggcgcgac gcaagagcgg acgcaaaaag tgttggatca agtgacggat tactatctga
    11461 agaacgagaa agcgaacgtt gaaagtgtct ttacagttaa cggctttagc ttcagcggcc
    11521 aggcacaaaa cgccggtatg gccttcgtca gtctgaaacc gtgggaagag cgtagtggtg
    11581 acgaaaacag tgcggaagca gtaatccatc gtgccaaaat ggaattgggc aagatccgcg
    11641 acggttttgt cattccattt aatatgccag ccattgttga actaggcacg gcaacgggtt
    11701 tcgactttga gttaattgat caggctgggc tgggtcacga tgccctgacc caggcccgta
    11761 accagttgct tggcatggcg gcgcaacatc ctgccagctt agtcagcgtg cgccctaatg
    11821 gcctggaaga caccgcgcag tttaaactgg aagttgacca ggaaaaggcg caggcattag
    11881 gtgtttcact ttctgacatc aatcagacca tttcaacggc gttgggtggg acttacgtta
    11941 acgacttcat cgaccgtggt cgcgtgaaaa aggtgtatgt tcaggcggat gccaaattcc
    12001 gtatgctgcc agaagatgtc gataaacttt atgtccgcag cgccaacggc gaaatggtgc
    12061 cattctctgc ctttaccact tcacattggg tgtatggctc tccgcgactg gaacgctaca
    12121 acggtctgcc gtcaatggag attcaggggg aagccgcgcc aggaaccagt tccggcgatg
    12181 ctatggcgtt gatggaaaac cttgcgtcaa aattaccggc gggcattggt tatgactgga
    12241 cgggtatgtc atatcaggaa cgtttatcgg gaaaccaggc tcccgctctg gtagcaattt
    12301 cctttgtggt tgttttcctg tgccttgctg cactctatga aagctggtca attcctgtct
    12361 cggttatgtt ggtcgtgccg ttagggattg tcggcgtgct actggcggca acactcttta
    12421 atcaaaaaaa tgacgtctac tttatggtgg gcttgctaac gacaattggc ttgtcggcca
    12481 aaaacgctat tttgattgtt gagttcgcta aagatctcat ggagaaagag ggtaaaggtg
    12541 ttgttgaagc gacactaatg gcagtacgta tgcgcctgcg tcctatcctg atgacctctc
    12601 tcgcctttat tctcggcgta ttaccgctag ctatcagtaa cggtgccggc agtggcgcgc
    12661 agaacgcagt gggtatcggg gtaatgggcg ggatggtttc tgcaacgttg ctggcaatct
    12721 tcttcgtgcc ggtgttcttt gtggtgatcc gccgttgctt taaaggataa atcagaaaca
    12781 taaaggcact ttcgggtgcc tttattattt ccagtgaaac ccataaaaat taataagata
    12841 ttcctctact cactttttaa aaactttcta tagttcccgc tcccttcact atttttacaa
    12901 ttcacataat ttgtcattat tcattccgca agatttataa cctccagtga taaaatggca
    12961 ttcagctcgt taataagaga gttaacttat taagcgttag cgtttat
//
LOCUS       DAJOTB010000133        12938 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJOTB010000133 DAJOTB010000000
VERSION     DAJOTB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.133"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(185..1549)
                     /gene="mnmE"
                     /locus_tag="OHN28_003765"
     CDS             complement(185..1549)
                     /gene="mnmE"
                     /locus_tag="OHN28_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCQ2299075.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1655..3301)
                     /gene="yidC"
                     /locus_tag="OHN28_003766"
     CDS             complement(1655..3301)
                     /gene="yidC"
                     /locus_tag="OHN28_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCQ2299076.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3304..3561)
                     /gene="yidD"
                     /locus_tag="OHN28_003767"
     CDS             complement(3304..3561)
                     /gene="yidD"
                     /locus_tag="OHN28_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCQ2299077.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3525..3884)
                     /gene="rnpA"
                     /locus_tag="OHN28_003768"
     CDS             complement(3525..3884)
                     /gene="rnpA"
                     /locus_tag="OHN28_003768"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCQ2299078.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3901..4041)
                     /gene="rpmH"
                     /locus_tag="OHN28_003769"
     CDS             complement(3901..4041)
                     /gene="rpmH"
                     /locus_tag="OHN28_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCQ2299079.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4271..4351)
                     /locus_tag="OHN28_003770"
     CDS             complement(4271..4351)
                     /locus_tag="OHN28_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299080.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            4648..6051
                     /gene="dnaA"
                     /locus_tag="OHN28_003771"
     CDS             4648..6051
                     /gene="dnaA"
                     /locus_tag="OHN28_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCQ2299081.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6056..7156
                     /gene="dnaN"
                     /locus_tag="OHN28_003772"
     CDS             6056..7156
                     /gene="dnaN"
                     /locus_tag="OHN28_003772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCQ2299082.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7156..8229
                     /gene="recF"
                     /locus_tag="OHN28_003773"
     CDS             7156..8229
                     /gene="recF"
                     /locus_tag="OHN28_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCQ2299083.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8258..10672
                     /gene="gyrB"
                     /locus_tag="OHN28_003774"
     CDS             8258..10672
                     /gene="gyrB"
                     /locus_tag="OHN28_003774"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCQ2299084.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10816..11214
                     /locus_tag="OHN28_003775"
     CDS             10816..11214
                     /locus_tag="OHN28_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCQ2299085.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11328..12140
                     /gene="yidA"
                     /locus_tag="OHN28_003776"
     CDS             11328..12140
                     /gene="yidA"
                     /locus_tag="OHN28_003776"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCQ2299086.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12184..12840)
                     /locus_tag="OHN28_003777"
     CDS             complement(12184..12840)
                     /locus_tag="OHN28_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299087.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
BASE COUNT         3122 a         3355 c         3492 g         2969 t
ORIGIN      
        1 attctgaaat tgtttaaatg tgaatcgaat cacaatcgtt cgggacgcaa tattaaatat
       61 ctattttaaa gattataaat tacgtaagaa ttttaatgtt taaaaaaggg ttgaggaata
      121 acaggagtgc taacaatgaa gcgaagccgc atctgacagt cagaatgcgg cttcgcaagc
      181 gcggttactt accgatacag aagctggaga aaatccgccc cagtaggtcg tctgaagtaa
      241 actccccggt gatctcgctt aagttctgct gcgccaggcg caactcttcc gccagcagtt
      301 cacccgccca ggcacctaac agttgcgctt taccttgctg caaatgctct gccgcctgtt
      361 ccagcgcctg taggtgacga cgacgcgcca ggaagccgcc ttccatatta gtgtcaaagc
      421 ccatgctctg tttgagatgg ttacgcagca catccacgcc ttcgccagtc cgcgccgaaa
      481 gacgaattaa cgcgtgaccg ttcacttcgc tcatccccaa cgtttcgccg gtgatatcgg
      541 ctttattgcg caccacggtg atcggcagtt tcgctggcag acgggcgata aattccggcc
      601 agatctctgc cggatcaacg gcgtctgtcg tggtgccatc gaccataaac agcacgcggt
      661 ctgcctgttc aatttcctgc catgcgcgct cgataccaat acgttcaact tcgtcactgg
      721 cttcacgtag cccggcggta tcgatgatat gcaacggcat tccgtcaatg tggatatgct
      781 cacgcagcac gtcacgcgtg gttccggcga tatcggttac gattgctgct tcacggcccg
      841 ccagcgcgtt cagcaggctc gatttaccgg cgttaggacg tccggcaatc accactttca
      901 tcccttcgcg caacaaacta ccctgacgag cttcggcgcg taccgcgtcg agatcggcaa
      961 ttacgtcatt gagctgggct tcaattttgc cgtcggagag gaaatcgatc tcttcatccg
     1021 ggaaatcaat tgccgcttcg acgtaaatgc gcaagtgggt gagggcttcc accagatggt
     1081 tgacccgtgc ggagaacgcg ccttgcagcg agtttagcgc cgaacgggcc gcctgttccg
     1141 agctggcgtc gataagatcc gcaatcgcct cggcctgggc taagtcgagt ttatcgttca
     1201 gaaatgcgcg ttcggaaaac tcaccagggc gggcaatccg caggccggga atggtcagaa
     1261 tgcgttttaa cagcaggtcg aggatcaccg gaccgccatg accttgcagt tccagcacat
     1321 cttcgccggt gaacgagttc gggccaggga accatagcgc aatcccctga tcgagcacgc
     1381 tgccatcggc gtctttaaac ggaagataat cggcgtagcg tggcttaggc agtttaccca
     1441 gcacggtttc ggcaacttca cgggctttga ggccggagat gcgcaggatg ccaacgccgc
     1501 cacgtcccgg aggcgtggcc tgggctacga tagtgtcatt atcgctcatg atgttcctgt
     1561 tgctttgtgt ggcggatgtg tggtgcttat ccgccctacg aaaagaaaaa aggcggtcaa
     1621 ctgaccgccc ttattttagc gaaaactcac cgaatcagga ttttttcttc tcgcggctat
     1681 gcaagccacg tttttccaga ccacggtaaa tcagctgctg ctgaataatg gttaccaggt
     1741 tgctgacgat atagtacagc accagacctg acgggaacca caggaagaac acagtgaaga
     1801 tgaccggcat aaaggtcatg atcttctgct gcatcgggtc ggtcacagtg gtcggcgaca
     1861 tcttctgaat gaagaacatc gttacgccca tcaggatcgg caggatgtag tacgggtcct
     1921 gtgcagacag gtcgtggatc cacagtgcaa acggtgcctg acgtagttca acggaaccca
     1981 tcagcatgta gtacaacgcc aggaagattg gcatctggat cagcagcggg aagcagccgc
     2041 ccagcgggtt aaccttctca gctttgtaca gcgccatcat ttcctggctt atgcgctgtt
     2101 tgtcatcgcc cagacgctca cgcattgcct gaatcttcgg ctgcagcata cgcatcttcg
     2161 ccatggaggt gtactgcgct ttggtcagcg ggtacatgat gccacgaacg ataaaggtga
     2221 tgatgataat ggagaagccc cagttaccca caaagctatg gatccatttc agcagtttga
     2281 acagtggctg agagatgaac cacaaccaac cgtaatcaac ggtcagatcc aggtgcggag
     2341 caacagctgc cattttgtcc tgaatttccg ggccaaccca cagggtgctg ttcatcgcgc
     2401 cagtctgacc aggctgaacc agtaccggct gagatttata gccgatagcg gcgatgccgt
     2461 tacccagatt agcggtgtag aagttgttgg taccgtcgtt atgcgggatc cacgccgtcg
     2521 cgaaatactg ttgcagcatt gccacccaac cacctttcga agagatgttc aggttttcgt
     2581 tatcggcaat ggtatcgaat ttgtatttct cgtacttctc gtcaggcgtg gagtacgccg
     2641 caccacggaa ggtgtgcagt gcgaagttgc tgcttccggt atcgagatgc ggtggcagag
     2701 tgatggattg cttcaactga ccaaaggtgg agatttccag cggtttctcg ccagcgttct
     2761 gcacgttgta gttgacgttg acagcgtaat caccacgttt caggacaaac gttttggtaa
     2821 acgtgttgcc tgccgcgtcg gtatacgtca tcggcacctg cagttcgttt tgaccttcag
     2881 ccagcacata agcgtctttt tcaacgttat atagcggacg cgggccgtta gccgggttat
     2941 ccgggccatc acgaccggtc agaccgctct gtgcctgata aataaactgc ggtgaagttt
     3001 ccagcagctg gaacggctgg gtagagttca gctctttcgg gtaagccggc agcagggcct
     3061 gctcaacatc accaccacgg gtgttgatgg tcagatcaag cacgtcggtc ttaaccgaga
     3121 tcagtttccc ctggccactg gccggtacgc cctggtcggc ggcgctaccc gctgcggtgg
     3181 tcgttgtctg cgtggtctgt tgggcctgag gttgcgggtt tttatcctgc tcccaggctt
     3241 gccagatcat gaaagacacg aacagcaaag cgatgactaa aagattgcgt tgcgaatcca
     3301 tcgttagtgt tctctggtat caaatggtcc gggcgggacg ggatcgtcac caccagggtg
     3361 taaagggtgg cattttaata cgcgtttcac cgtcaaccaa ctgcctttta tcactccaaa
     3421 cctgcgcaat gcctcaattc cgtagcttga acaggttgga gtgaaacgac aatgcggccc
     3481 gagtagcgga ctaatcaggc gttgatagac ccgaatgagg gctatcagga cccgcgagcc
     3541 aggcgacagt ggcggcgcca taatttttcc aacgcttccg agagagcacg gttatcgagg
     3601 tcggcaaccc cttttttcgc caccaccacg aaatccatag ccgggagttc atgttggcgc
     3661 agacggaagc tttcacgcgt cagacgttta atccgattgc gttcatgggc gcgtcgaacg
     3721 tttttcttgg cgactgtaag accgatacgg ggatgcccca gcgaattcag gcggccgaga
     3781 atagtaattt gcggcgtgcc agcccgttgt ggctgctgga agacgaatgt gaattgactg
     3841 ggagttaaca agcgtaactc cctgggaaat gcgagcttaa ccactcaggg gttagcttta
     3901 ttacttagaa acggtcagac gagcgcggcc tttagcacga cgacgtgcca gaacctgacg
     3961 accattttta gtagccatac gagcacggaa gccgtgagaa cggttgcgct tcagtacaga
     4021 cggttgaaaa gtgcgtttca tggcgatttc tacctaaact tgaataaatt caatggcttt
     4081 attggatatc cgccgaaaaa tgaaacgatg gacaccgaag ccatgggtga ttaaagaggc
     4141 cggattgtaa taattgtaca ctccggagtc aattctcttt ccttatttac cgcgcttttc
     4201 cgcacctttt cgcggggaaa acgtacggcc tcacaccagt gaaaaccagc atggcgcgcc
     4261 gggtggagga ttatacgggc tgacgggtaa agcgcaagga tcgtcctgga tctttattag
     4321 atcgattaag ccattttttg tctatggtca ttaaattttc caatatgcgg cgtaaatcgt
     4381 gcccggcctg cggcaggatc gtttacactt agcgagttct ggatcatcct gtggataaat
     4441 cgggaaaatc tgtgagaaac agaagatctc ttgcgcagtt taggctatga tccgcggtcc
     4501 cgatcgtttt gcaggatctt gatcgggcat ataaccgcag acagcggttc gtgcgtcacc
     4561 ctcatgcagg gtcttttcga cgtacgtcaa caatcatgaa tgtttcagcc ttagtcatta
     4621 tcgacttttg ttcgagtgga gtccgccgtg tcactttcgc tttggcagca gtgtcttgcc
     4681 cgattgcagg atgagttacc agccacagaa ttcagtatgt ggatacgccc attgcaggcg
     4741 gaactgagcg ataacacgct ggccctgtac gcgccaaacc gttttgtcct cgattgggta
     4801 cgggacaagt accttaataa tatcaatgga ctgctaacca gtttctgcgg agcggatgcc
     4861 ccacagctgc gttttgaagt cggcaccaaa ccggtgacgc aaacgccaca agcggcagtg
     4921 acgagcaacg tcgcggcccc tgcacaggtg gcgcaaacgc agccgcaacg tgctgcgcct
     4981 tctacgcgct cgggttggga taacgtcccg gctccggcag aaccgaccta tcgttctaac
     5041 gtaaacgtca aacacacgtt tgataacttc gttgaaggta aatctaacca actggcgcgc
     5101 gcggcggctc gccaggtggc agataaccct ggcggtgctt ataacccgtt gttcctttat
     5161 ggcggcacgg gtttgggtaa aactcacctg ctgcatgcgg tgggtaacgg cattatggcg
     5221 cgcaagccga atgccaaagt ggtttatatg cactccgagc gctttgttca ggacatggtt
     5281 aaagccctgc aaaacaacgc gatcgaagag tttaaacgct actaccgttc cgtagatgca
     5341 ctgctgatcg acgatattca gttttttgct aataaagaac gatctcagga agagtttttc
     5401 cacaccttca acgccctgct ggaaggtaat caacagatca ttctcacctc cgatcgctat
     5461 ccgaaagaga tcaacggcgt tgaggatcgt ttgaaatccc gcttcggctg gggactgact
     5521 gtggcgatcg aaccgccaga gctggaaacc cgcgtggcga tcctgatgaa aaaggccgac
     5581 gaaaacgaca ttcgtttgcc gggcgaagtg gcgttcttta tcgccaagcg tctacgatct
     5641 aacgtacgtg agttggaagg cgcgctgaac cgcgtcattg ccaacgccaa ctttaccgga
     5701 cgggcgatca ccatcgactt cgtgcgtgag gcgctgcgcg acttgctggc attgcaggaa
     5761 aaactggtca ccatcgacaa tattcagaag acggtggcgg agtactacaa gatcaaagtc
     5821 gcggatctcc tttccaagcg tcgatcccgc tcggtggccc gtccgcgcca gatggcgatg
     5881 gcgctggcga aagagctgac taaccacagt ctgccggaga ttggcgatgc ctttggtggt
     5941 cgtgaccaca cgacggtgct tcatgcctgc cggaagatcg agcagttgcg tgaagagagc
     6001 cacgatatca aagaagattt ttctaattta atcagaacat tgtcatcgta aacctatgaa
     6061 atttaccgta gaacgtgagc atttattaaa accgctacaa caggtgagcg gtccgttagg
     6121 tggtcgtcct acgctaccga ttctcggtaa tctgctgtta caggttgctg acggtacgtt
     6181 gtcgctgacc ggtactgatc tcgagatgga aatggtggca cgcgttgcgc tggttcagcc
     6241 gcacgaacca ggggcgacga ccgttccggc gcgcaaattc tttgatatct gccgtggtct
     6301 gcctgaaggc gcggaaattg ccgtgcagct ggaaggtgag cggatgctgg tgcgctccgg
     6361 gcgtagccgt ttttcgctgt ctaccctgcc agcggcagat ttcccgaacc tcgatgactg
     6421 gcagagcgaa gtcgaattta ccctgccgca ggcgacgatg aagcgtctga ttgaagcgac
     6481 ccagttttct atggcacatc aggacgttcg ctattactta aacggtatgc tgtttgaaac
     6541 cgaaggtgaa gaactgcgca ccgtggcgac cgacggccac cgtctggcag tctgttcaat
     6601 gccaattggt caatctttgc caagccattc ggtgatcgtg ccgcgtaaag gcgtaattga
     6661 actgatgcgt atgctcgacg gcggcgacaa tccgctgcgc gtgcagattg gcagcaacaa
     6721 cattcgcgcc cacgttggcg actttatctt cacctcgaaa ctggtggatg gtcgcttccc
     6781 ggattaccgt cgcgttctgc cgaagaaccc ggacaagcat ctggaagctg gctgtgatct
     6841 gctcaagcag gcgtttgccc gtgcggcaat tctctctaac gagaaattcc gcggcgtgcg
     6901 tctgtatgtc agcgaaaacc agctgaaaat caccgccaac aacccagaac aggaagaagc
     6961 ggaagagatc ctcgacgtta cctatagcgg tgcggagatg gaaatcggct tcaacgtcag
     7021 ctatgtgctg gatgttctga acgcactgaa atgcgaaaac gtccgcatga tgctgaccga
     7081 ttcggtttcc agcgtgcaga ttgaagatgc ggccagccag agcgcggctt atgttgtcat
     7141 gccaatgaga ctgtaatgtc cctcacccgc ttgttgatcc gcgatttccg caacattgaa
     7201 accgcggatc tcgccttatc tcccggcttt aactttctgg taggtgccaa cggcagtggc
     7261 aaaaccagcg tgctggaagc catctatacg ctcggccacg gtcgggcgtt tcgcagtttg
     7321 cagattggtc gcgtcattcg ccacgagcag gaggcgtttg ttctccacgg acgattacag
     7381 ggcgaagagc gcgagacggc gattggttta accaaagaca aacaaggcga cagcaaagtc
     7441 cgtattgacg gtacagacgg gcataaagtc gcggagctgg cgcacctgat gccaatgcag
     7501 ctgataacgc cagaagggtt tactttactc aatggcggcc ccaaatacag aagagcattc
     7561 ctcgactggg gatgctttca caacgaaccc ggatttttca ccgcctggag caatctcaag
     7621 cgattgctca agcagcgcaa tgcggcgctg cgccaggtga ctcgttacga acagctacgc
     7681 ccgtgggata aagaactgat cccgctggcg gagcaaatca gcacctggcg cgcggagtat
     7741 agcgccggta tcgcagccga tatggctgat acctgtaagc aatttctccc tgagttttct
     7801 ctgactttct ctttccagcg cggctgggag aaagagacag agtacgctga ggtgctggaa
     7861 cgtaattttg aacgcgatcg ccagttaacc tacaccgcgc acggcccgca taaagcggac
     7921 ttacgcattc gcgccgacgg tgcgccggtg gaagatacct tatcgcgtgg acagcttaag
     7981 ctgttgatgt gcgccttacg tctggcgcaa ggagagttcc tcacccgtga aagcgggcgg
     8041 cggtgtctct acctgataga tgattttgcc tctgagcttg atgatgagcg tcgcgggctg
     8101 cttgccagcc gcttaaaagc gacgcaatca caggtctttg tcagcgcgat cagtgctgaa
     8161 cacgtaatag acatgtcgga cgaaaattcg aagatgttta ccgtggaaaa gggtaaaata
     8221 acggattaac ccaagaataa atgagcgaga aacgttgatg tcgaattctt atgactcctc
     8281 cagtatcaaa gtcctgaaag ggctggatgc ggtgcgtaag cgcccgggta tgtatatcgg
     8341 cgacacggat gacggcaccg gtctgcacca catggtattc gaggtggtag ataacgctat
     8401 cgacgaagcg ctcgcgggtc actgtaaaga aattatcgtc accattcacg ccgataactc
     8461 tgtctctgta caggatgacg ggcgcggcat tccgaccggt attcacccgg aagagggcgt
     8521 atcggcggcg gaagtgatca tgaccgttct gcacgcaggc ggtaaatttg acgataactc
     8581 ctataaagtg tccggcggtc tgcacggcgt tggtgtttcg gtagtaaacg ccctgtcgca
     8641 aaaactggag ctggttatcc agcgcgaggg taaaattcac cgtcagatct acgaacacgg
     8701 tgtaccgcag gccccgctgg cggttaccgg cgagactgaa aaaaccggca ccatggtgcg
     8761 tttctggcca agccttgaaa ccttcaccaa tgtgaccgag ttcgaatatg aaattctggc
     8821 gaaacgtctg cgtgagttgt cgttcctcaa ctccggcgtt tccattcgtc tgcgcgataa
     8881 gcgcgacggc aaagaagacc acttccacta tgaaggcggc atcaaggcgt tcgttgaata
     8941 tctgaacaag aacaaaacgc cgatccaccc gaatatcttc tacttttcca ccgaaaaaga
     9001 cggtattggc gtcgaagtgg cgttgcagtg gaacgatggc ttccaggaaa acatctactg
     9061 ctttaccaac aacattccgc agcgtgacgg cggtactcac ctggcaggtt tccgtgcggc
     9121 gatgacccgt actctgaacg cctacatgga caaagaaggc tacagcaaaa aagccaaagt
     9181 cagcgccacc ggtgacgatg cgcgtgaagg cctgattgcg gtcgtttccg tgaaagtgcc
     9241 ggacccgaaa ttctcctcac agaccaaaga caaactggtt tcttctgagg tgaaatcggc
     9301 ggttgaacag cagatgaacg aactgctggc ggaatacctg ctggaaaacc caaccgacgc
     9361 gaaaatcgtg gttggcaaaa ttatcgatgc tgcccgtgcc cgtgaagcgg cgcgtcgcgc
     9421 gcgtgaaatg actcgccgta aaggtgcgct cgacttagcg ggcctgccgg gcaaactggc
     9481 agactgccag gaacgcgacc cagcgctttc cgaactatac ctagtggaag gggactccgc
     9541 gggcggctct gcgaagcagg ggcgtaaccg caagaaccag gcgattctgc cgctgaaggg
     9601 taaaatcctc aacgtcgaga aagcgcgctt cgataagatg ctctcttctc aggaagtggc
     9661 gacgcttatc accgcgcttg gttgtggtat cggtcgtgac gagtacaacc cggacaaact
     9721 gcgttatcac agcatcatca tcatgaccga tgcggacgtc gacggctcgc acattcgtac
     9781 gctgctgttg accttcttct atcgtcagat gccggaaatc gttgaacgtg gtcacgtcta
     9841 catcgctcag ccgccgctgt acaaagtgaa gaaaggtaag caggaacagt acattaaaga
     9901 cgacgaagcg atggatcagt accagatctc tatcgcgctg gatggcgcaa cgctgcacac
     9961 caacgccagt gcaccggcgc tggctggcga agcgttagag aaactggtgt ctgagtacaa
    10021 cgcgacgcag aaaatgatca accgcatgga gcgtcgttat ccgaaagcaa tgctgaaaga
    10081 gcttatctat cagccgacgc tgacggaaac cgacctttct gacgagcaaa ccgttacccg
    10141 ctgggtgaac gcgctggtca gcgaactgaa cgataaagaa cagcacggca gccagtggaa
    10201 gtttgatgtc cataccaatg ccgaacaaaa cctgttcgag ccgattgttc gcgtgcgtac
    10261 ccacggtgtg gatactgact atccgctgga tcacgaattt atcactggcg gcgaatatcg
    10321 tcgtatctgc acgctgggtg agaaactgcg tggcttgctg gaagaagatg catttatcga
    10381 acgtggcgaa cgtcgtcagc cggtagccag cttcgagcag gcgctggact ggctggtgaa
    10441 agagtcccgt cgcggcctct ccatccagcg ttataaaggt ctgggcgaga tgaacccgga
    10501 acagctgtgg gaaaccacca tggacccgga aagccgtcgt atgctgcgcg ttaccgttaa
    10561 agatgcgatt gctgccgacc agttgttcac tacgcttatg ggcgacgccg ttgaaccgcg
    10621 ccgtgcgttt atcgaagaga acgctctgaa agcggcgaat atcgatattt aatggctttt
    10681 tgttgctgat aaaatgactt agtcgtcagt ccgggcggag atcatctccg ccctttttat
    10741 tcctgcaatc cgcaattatc atcccgcaga tctatacttt ttgggtcaac acgtaacgct
    10801 ttacaggtga ttatcatggg gctttttgat gaagttgtcg gtgcctttct gaaaggcgat
    10861 gcggggaaat atcaggcgat tttgagctgg gttgaggagc agggcggcat tcaggtgctg
    10921 ctggaaaaac tgcaaagtgg cggcttaggg gccattctct caacctggct gagtaatcaa
    10981 cagagcaatc aatcagttag cggcgagcaa gtggaatcgg cgctcggcac aaatgcggtg
    11041 tccgatctcg ggcaaaaact tggcgtggat accagtacag cttccagttt actggcagaa
    11101 caattgccga agattattga tgcactctca ccgcaaggtg aagtgtcacc acaagccaat
    11161 aacgatctgc tttccgcagg tatggaactg ctgaaaggga aactcttccg ctaagcaaat
    11221 ggggagcacg caatgcgcgt tgcatcccct tttccctcgg gtgttgtact gatttttgag
    11281 cggaatcgcg ttagcatggg tcaggaacca atctacctgg ggaactcatg gctatcaaac
    11341 tcattgctat cgatatggat ggcacccttc tgctgcccga tcacaccatt tcacccgccg
    11401 ttaaaaatgc gattgccgca gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc
    11461 gcccttatgc aggtgttcac aattacctga aagagctgca tatggaacag ccgggcgact
    11521 actgcattac ttataacggc gcgctggtac agaaggccgc tgatggtagc accgtggcgc
    11581 aaaccgctct cagctatgac gactaccgtt tcctggaaaa actctctcgc gaagtcggtt
    11641 ctcatttcca cgccctggac cgcaccacgc tgtacaccgc caaccgagat atcagctact
    11701 acacggtgca tgaatctttc gttgccacca ttccgctggt gttctgcgaa gcggagaaaa
    11761 tggaccccaa tacccagttc ctgaaagtga tgatgattga tgaacccgcc atcctcgacc
    11821 aggccattgc gcgtattccg caggaagtga aagaaaaata caccgtgctg aaaagtgcgc
    11881 cgtacttcct cgaaatcctc gataaacgcg ttaacaaagg tacgggtgtg aaatcactgg
    11941 ccgacgtgtt aggtattaaa ccggaagaaa tcatggcgat tggcgatcag gaaaacgaca
    12001 tcgcaatgat tgaatatgca ggcgtcggtg tggcgatgga taacgctatt ccttcggtga
    12061 aagaagtggc gaactttgtc accaaatcta accttgaaga tggcgtggca tttgctattg
    12121 agaagtatgt gctgaattaa tcagtgaacg ggcaaacggc atgaatgcgt ttgcccgcag
    12181 cgattaacaa tttctggtcg tgcataaaat caggaactgg agtggtgacg ttactatatc
    12241 cagcgtaaga gtgactggca gcagcgcatt aacgaggccg cgcgggacgc catctgttgc
    12301 tttgtactca taaaatccca ccgtaaaggg ttgatggaaa accatcacgt ttgagtaatc
    12361 cgctttttta tttttctggt ggattgagcc tttcaaaccg tccagcatcc atgtacaacg
    12421 ccgggtatca caaataaaac catattgtgt tgccagtgcc ttatcttctt cgttgttcca
    12481 gtcgtaatag agcgtaatag caccagagaa attcttatta ctgctgctta aaataaattt
    12541 cgcgttatca gaaacggtga ttctttcacc atggattgcc ggatcgttca gcatgttaac
    12601 cacctcatcg ccgccggtgg tcaatagata gtccaacgat tcaccaacaa aaacataacc
    12661 tttcgtaccg ttactgtctt cagccagcga taaaccctta atcgtgtctg accgccattg
    12721 ctcggcatgt ggacggcttg cagtattgga aaccaatccc caggtgatac atccggtcaa
    12781 cgcaaatgcc aaagaacaca aaccggcagc cttaaaaaat cctttaaaat tcaatttcat
    12841 gattaatcct ttaaacaaga gtgctaagcc gttgaaagca tactcttctt tccctcctgt
    12901 ttagcgcctg atctcccttt tcagattttt gcccgacg
//
LOCUS       DAJOTB010000134        12660 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJOTB010000134 DAJOTB010000000
VERSION     DAJOTB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.134"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(157..930)
                     /gene="uxuR"
                     /locus_tag="OHN28_003778"
     CDS             complement(157..930)
                     /gene="uxuR"
                     /locus_tag="OHN28_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCQ2299088.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1145..2605)
                     /locus_tag="OHN28_003779"
     CDS             complement(1145..2605)
                     /locus_tag="OHN28_003779"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCQ2299089.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2686..3870)
                     /gene="uxuA"
                     /locus_tag="OHN28_003780"
     CDS             complement(2686..3870)
                     /gene="uxuA"
                     /locus_tag="OHN28_003780"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCQ2299090.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFELHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4212..5555
                     /gene="gntP"
                     /locus_tag="OHN28_003781"
     CDS             4212..5555
                     /gene="gntP"
                     /locus_tag="OHN28_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCQ2299091.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQI
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5798..6700)
                     /gene="fimH"
                     /locus_tag="OHN28_003782"
     CDS             complement(5798..6700)
                     /gene="fimH"
                     /locus_tag="OHN28_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCQ2299092.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGVVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6720..7223)
                     /gene="fimG"
                     /locus_tag="OHN28_003783"
     CDS             complement(6720..7223)
                     /gene="fimG"
                     /locus_tag="OHN28_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCQ2299093.1"
                     /translation="MKWRKRGYLLAAMLAFASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7236..7766)
                     /gene="fimF"
                     /locus_tag="OHN28_003784"
     CDS             complement(7236..7766)
                     /gene="fimF"
                     /locus_tag="OHN28_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCQ2299094.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7776..10412)
                     /gene="fimD"
                     /locus_tag="OHN28_003785"
     CDS             complement(7776..10412)
                     /gene="fimD"
                     /locus_tag="OHN28_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCQ2299095.1"
                     /translation="MSYLNLRLYQRNTQCLHIRRHRLAGFFVRLFVACAFTAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYVGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10478..11203)
                     /gene="fimC"
                     /locus_tag="OHN28_003786"
     CDS             complement(10478..11203)
                     /gene="fimC"
                     /locus_tag="OHN28_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCQ2299096.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11240..11779)
                     /locus_tag="OHN28_003787"
     CDS             complement(11240..11779)
                     /locus_tag="OHN28_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2299097.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11844..12392)
                     /gene="fimA"
                     /locus_tag="OHN28_003788"
     CDS             complement(11844..12392)
                     /gene="fimA"
                     /locus_tag="OHN28_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2299098.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLDQDGATSSAVGFNIQLNDCDTDVASKAAVAFLGTTID
                     TNHTNVLALQSSAAGSATNVGVQILDKTGTALALDGASFSAQTTLNNGTNTIPFQARY
                     YAIGAATPGAANADATFKVQYQ"
BASE COUNT         3017 a         3319 c         3086 g         3238 t
ORIGIN      
        1 ataatttttc agcgttaaac tgatatataa ttattccaaa acaatatatc gtacgtaatg
       61 attaatttac gcataatata ttctgcagaa cataattaac tattcaggga acatacatag
      121 tgcaaagcgg ttaaactttg caccagaaat gagggatcag gcgtcgactt tatccagcgg
      181 ccatgaatca aacagatagc catcgaaata gatgtcatca acattagaga actccagcaa
      241 gcgctgcttc acattctcca gatgctgcca cattgccagc ttcgccgctc gggcatcttt
      301 tttgattaac gcggcgagga tctgtttgtg atcgcccaac cactctttgc gatacaggct
      361 gtcatccagg tggctgtgca actggatcca cattggattg ttttcccgcc actgccagga
      421 ctgacggaat aactccacca gcatgctgtt atgcgttgct tcggcaatag cgagatggaa
      481 ctgcatgtca ccgctttcgc tgctgcccgg cgcgctggaa gccagttcac gctcttccag
      541 ttgcaatgcc tggcgcattt tgacgatatc ttcgcgggta gcctgcaggg cggcaaactc
      601 ggcgatgtta ctttccagta actgccgcgc ctgtaacagc tcaaaaggac cggcatcgtt
      661 gcagacgttg gcatccggac tgtctgcgtt gtggctgcct gagctatcaa gaacatagat
      721 acctgcaccc cgacgcactt ccaccagccc tttgatctcc agcatgatca gcgcttcacg
      781 taccaccgtc cgcgtgacat caagcatttc tgcaatttca cgctccggtg gcagccgttc
      841 gccaggattg tacggcgtct ttatgatcag atcgcggatc atcgccccga cttcctggta
      901 aggtctttgc gcagaggtgg cagatttcat ggtgttgtgt ccgttaggtg cggttaatca
      961 aattttttcc ggatgcggcg tgaacgcctt atccgaccta caacgggcaa ttttacggca
     1021 cccgttaagc tcaaactata ccattagcac tggatgttgg cacgtacagg tgggcaaaaa
     1081 aacgaaaact ggtcaaccaa atattagttt gattgaccag taagtctgta gggtaatcag
     1141 ttagttacag cgcagccaca cactcgcgcg caccgcgctc acacagctgc tgatatgccg
     1201 ccgtcaccgc gccaacaaag tcggcgttct gcggcagatc atcggcaaag ataccgctca
     1261 ggcccagcag tgctttcacg cggtctgcac cctgatactg cgcgttgatc ttctggaact
     1321 ccgccagcat cgggtcaacc acgtcaatgg cattaccctg ctcatccacg ccctgggtgt
     1381 agcgcatcca gcccgccacg cccagcgcca ggtgacgcca gctgccgccg ttttgcaggt
     1441 gcagacgcac agggtccaac agacgctgcg gtaacttctg gctgccgtcc attgcaatct
     1501 gccaggtacg gtgacgcaga gacgggttgc tgaaacgctc gatcagcagc gtcgcatagg
     1561 cgttcaggtc cgtaccttcc ggcatcgaca gcgttggcgc ttgttcctgc atcatcaggg
     1621 caaaggccgc tttgcgataa gccgggttag tcatggtgtc ggcaatagtt tcatagccgc
     1681 cgaggtaacc gaggtacgcc aggaaagagt ggctaccgtt cagcatacgc agcttcatca
     1741 tttcgaacgg tacaacatcc gcaacaaact gcgcgccgac tttatcccag tccggacgac
     1801 cattaacgaa gttatcttca attacccact gacggaacgg ttcgcaggca atggcgcacg
     1861 ggtcgtaaac acccagctgg tcggcaattt cctgtaaggt ttctggcgtc gccgccggaa
     1921 cgatgcggtc aaccatggtg cacgggaagg tgacgttctc ttcaatccat gccgccagtt
     1981 gcgggtcacg cgcctgagcc agcccaagaa cggcaacctt cgccacatga ccgttttcac
     2041 gcacgttatc gcaggacatc accgtaaacg ctttcagccc tttttcacga cgcagacgca
     2101 gggcttctac gatgtaacca atcgcagact tcggcgcagt cgggttttcc agatcgtgct
     2161 tgatcagcgg gttattgaga tccagctgac cgcttgccgc atcagcgcag tagccttttt
     2221 ccgtgaccgt cagagagaca atcgccgtct gcggacgcgc catcgcgttg agaatacctt
     2281 cgcaaccgtc gatttccgga tgcagcgctt ctttcatcga accgataatt ttcagctcgg
     2341 tgctttccgc gcctttttcc gccacggtgt acagcagttg ctgttttttc aggttttcga
     2401 tcagcacgcg gtcgttgcct ggcatcaggt taacttcgca gataccccag tcgctgtcgg
     2461 tactttccag cagatgatgg gtatacagcg cctggtgcgc gcggtgaaac gccccgcaac
     2521 cgagatgcac aatgcgtgat tccagacgag aatgatccca tgacgggcgg gcaaccggca
     2581 gattgctgtc aacaatagta gtcatcgtaa actccagaat ccgtcttcag agagtgacgg
     2641 caaggaagaa ggaaccggca cggcgcgcag ccatgccggt ggatattaac ggctaaagaa
     2701 agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc aggcctttca gacgaccaat
     2761 tgcggagtaa ccaggattgg ttttcttctt caggtcgtcc agcatctgat gaccgtggtc
     2821 cggacgcatc gggatcaggt cttctttgcc ttcagcttta cgacggtgtt cttcttcaac
     2881 aatcgctttc accacttcgt acatatcaac gtcaccgttc aggtgcgccg cttcgtggaa
     2941 ggttttcggg ttatcttcac gcatggtgga gcgcagatgg gtgaagtaaa tacgcggacc
     3001 gaactgcttg atcatatcaa ccagatcgtt gtcagcacgc acgccgtagg aaccggtgca
     3061 catggtgaaa ccgttcgcca tgctgtttac ggtatcaacc atccactgca tatcttcaat
     3121 ggtggaaaca atgcgcggca ggccgaggat cgggcgcggc ggatcgtccg ggtgaacagc
     3181 catacggacg ccaacttctt cggcaaccgg aataatggct ttcaggaaga cagcaaagtt
     3241 ttcgcgcagt ttggctttgt cgatatcttt gtacagctcc aggtgcttac ggaactggtc
     3301 gagggtataa ccttcttccg cgcccggcag accggcgatg atgttacggg tcagacgcgc
     3361 tttgtcttcg tcgctcatgg tagcgaagcg aactgctgcc tgagcaattt cttcttcggt
     3421 gtaatccgct tctgcgcctg gacgcttcag gatgtgcagt tcaaatgctg caaattcgat
     3481 ctggtcgaaa cgcagcgctt tggagccgtc tggcagcacg tattcgaggt cagtacgagt
     3541 ccagtcgagc accggcatga agttgtagca cacggtgcga atgccgcact gcgccaggtt
     3601 gcgcagagtc tgctgatagt tagcgatcca ctgctcatag ttgccggtgt gggttttgat
     3661 atcttcgtga attggcacgc tttctacgac agaccatacc aggcctgcgt cttcaacgat
     3721 cgccttgcgt ttgaggatct cttcaacgga ccatacttcg ccattcggga tatggtgcag
     3781 cgcggtaacc acgccagttg cgcccgcctg acggacatca gctaaagaaa ccggatcgtt
     3841 tgggccgtac cagcgccagg tctgttccat gatttttcct ctttcacata gtgggttcaa
     3901 cagcgaagag acgcggaacc ccggagttgt ttaggtatcc ggatgaacaa cccggccaca
     3961 gccgtagcgt cgcatcggat gagatggcgt ataagttcta ccttttggta agacatgtca
     4021 acccgaattc taaaattggt taaccacatc acaagaattt tactctcacg gttgttgctg
     4081 caaattgcag caaagggtaa tcgatggtta ttgatcagag gatgtgacat tcaccgcaac
     4141 aatggttgac caatttacat aacatgtcgc ccaaaataac aacggttcaa ccaggtaaaa
     4201 ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt attcggcatt ggcctgatgc
     4261 tggtactgaa tttgaagttc aaaatcaatt cgatggtggc tttgttggtg gcggcgctgt
     4321 ccgtcgggat gctggccggt atggatttga tgtcgctgct gcacaccatg aaagcaggtt
     4381 tcggcaacac gctgggggaa ctggcgatca tcgtggtgtt tggtgcggtc atcggtaaat
     4441 tgatggtcga ctccggcgct gctcaccaga tagcgcatac cctgctggca cgtctcggcc
     4501 tgcgctatgt tcagatatca gtgattatca tcggacttat ttttggtctg gcgatgttct
     4561 atgaagtggc ctttattatg ctggcgccgc tggtgatcgt aattgccgcc gaagccaaaa
     4621 taccgttcct gaaactggct attccggcag tagcggcggc caccaccgca cattcactgt
     4681 tccctccgca accgggtccg gtggcgctgg tgaatgctta cggcgcagat atggggatgg
     4741 tttatatcta tggcgtcctg gtgactattc ccagcgtaat ctgtgcggga ctgatcttgc
     4801 cgaaattcct cggcaacctt gagcgcccga caccgtcatt tctgaaagcg gatcaaccgg
     4861 ttgatatgaa caacctgccc tcttttggtg tctcgattct ggtgccgctg atcccggcga
     4921 tcattatgat ctccaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg
     4981 aagtggttaa ctttatcggt tcctcgccga ttgcaatgtt tattgcgatg gtggttgcat
     5041 tcgtactctt tggtaccgcg cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa
     5101 gcgcggtgaa gagcattgca atggtgattc tgatcatcgg tgcgggtggc gtgctgaagc
     5161 agaccatcat cgacaccggc attggtgaca ccatcggcat gttgatgtcc cacggcaata
     5221 tctcgcccta cattatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg
     5281 gtgtcgtttc ggcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa
     5341 ctggtcagct gattggcgtg aatccggcgc tgctggtgct ggcgacggct gcgggttcca
     5401 acaccctcac ccacattaat gatgcctcat tctggctgtt caaaggttac tttgacctgt
     5461 cagtaaaaga cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc
     5521 tgattattgt gttgattatc agcatggtag cgtaaaagag caccagagcc tgccattggc
     5581 aggctctgac tgatgacaaa gtctaaaaat gtgcccggcc agtcccctcg cccctccggg
     5641 gagagggtta gggtgagggg aacagaccag caaagtgcgc tttgtttatg ccagatgcga
     5701 cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc aggtttttag cttcaggcaa
     5761 tattgcgtac ctgcattagc aatgccctgt gatttcttta ttgataaaca aaagttacgc
     5821 caataatcga ttgcacattc cctgcagtca cctgccctcc ggtacgtgcg taatttgccg
     5881 ttaatcccag acttaccgcc gaagtcccta ctactcctaa cgataccgtg ttattcgctg
     5941 gaataatcgt accgttgcgc gtcaactgta cgccgacgcc ctgcgctggt gaaaacgacg
     6001 cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg taatacccca
     6061 ggttttggct tttcgcacaa taaacggtaa gaggaatcgg cactgaacca gggtagtccg
     6121 gcagagtaac ggtgacatca cgagcagaaa catcacagcc gccagtaggc accaccacat
     6181 cattattggc gtaaatattc cacacaaact ggaaatcatc gctgttatag ttgttggtct
     6241 gtcgcaaaat tagcacggca attaatgagc cagctttaat cgccactccc cccgcactgc
     6301 tcacaggcgt caaataaagc gccaccggcc acggcttatc cgttctcgaa ttataaacaa
     6361 cccgcggcgt ttcgctggta gtagggaaag gatagctact gccattatat tttacggtcc
     6421 cggaaaaatt agataacacg ccgccataag ccgagcctcg ttgcagtgtg acataatctg
     6481 taatggtttc cgggtaatcg ttatggcaaa agatttgcgt cgaaagatcc acgaccaggt
     6541 tttgccccac attcacggca ggcgcaaggt ttacataaac attggcgctg ccaccgccaa
     6601 tagggattgc ggtaccattg gcggttttac aggcgaatga ccaggcattt accgaccagc
     6661 ccatcagcag tacagcaaac agggtaataa ctcgtttcat tacaatcatc tctttgggtt
     6721 cagctgtagg tataggtaat gctaatcact gcctgaatgg ttccctgagt ggctccgcca
     6781 tttaccgtca atgctctgac ctgtaacggg aagtgcgctg attgtgagga atcatccacc
     6841 tgaactgttt tggttgcgcc agtattcaat gtgttgccac tgtcatcctg tagctctaac
     6901 tggatgtttt gcgcggtccc ctgattttta taatatccgg tactgtcggc tgccccgctg
     6961 aagctggcag tgaccctcga cgttcccacc ggacaattag tcaactcaag cgcaacatca
     7021 tgccaggccg atgccgcccc ggcagacatc agactgaaag aataaagatc gccgagatca
     7081 accgtggcat tggtggtgga aaccgtacac ggtttggcga cgaccttacc gttcaccgtg
     7141 atggtgacat cggctgcctg tatcgtcgca cttgcgaacg ccaacattgc cgccaataaa
     7201 tacccacgtt tgcgccattt catgagcatc tccagttact gatattcaag agtgaaggta
     7261 gccgtggcat tgatatgccc cgcagtgaca ggcacctgtg tcgccattag ccgggcgtaa
     7321 aaattcaacg tatttggttt acccggcgtc agggtcgtcc acgaaatcgc ggacgatggg
     7381 gcattaaggg gtatctcatt ttgctgctca ttcagaagct gtattcccag tcccgcagcc
     7441 gctgacaccg tattttcaag tgcaagcagg ttggcattgt ggctatctgc aacgccagta
     7501 aacccaacct ttacggcaga aacagcgtta ccacagggtg acagcaaaat acgaaatgga
     7561 acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc catcaaatca
     7621 acagtaaaat tggttgattc agcggccaca ctacagccgt tatctctgac atagccgcgg
     7681 atagtaatcg tgctatccgc agccaaagcg tgactcaccg ccagccacaa aaaagcgcac
     7741 agaagataaa aaggtttgtt tctcatcacg cccccttaac gacattcagc tgatagctgg
     7801 gttaataact gctgctgact ctctggtggc agttgataat tggcgacaca gtgagcattt
     7861 tcctcttctc cccatttcac ctgaactttt cccgctaaag gcattccgct gaggtaaacc
     7921 tgaccattat ccgcaacaat gccgctactc tggctactct ctgatgtcac catcgcccca
     7981 aacggcagcg gcttattatt gtgggttagc gtcatgagca gttttatccc aacgcgcgct
     8041 ttaaactctg ctcgcacgat cgccccacgg gtgggaacaa cgttagcgac cgcattatct
     8101 aaatcgacgt tatcagccag ggtattggta tccagcgcca ctctgttttc ccgatattca
     8161 gtggcatatg gcagcacggc ataaccccgc cagtcggtac gcactcctgt ctggttttcg
     8221 acttttgcat cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt taacggctgc
     8281 cccagcgtta cgccattggc atgagccagt accccaccgc tgactccgta atagagctgc
     8341 ttaatatcat cgctatggct gtaaccgatg ttggcattgc cgtagccacc gcgataattc
     8401 agcgtggcgt agcctgtact tccgctattt ccatcgcctc ccccgacata accggtttgc
     8461 acgctatagc tgaggttgtt gtcttccagc aacgtaccgt atacaccggc cagattggtc
     8521 atccgaccgt tgagatcgtg tgacatgctg tagctggcac tggcatgtcg ccactgagat
     8581 ttactgtcag aacgcagcca gtggctgaaa ggaatattga cgttaagagc taacatctga
     8641 tcccgtcctt tttgccaggc gtttttcgtc aggctatagc tgagcgtcca gttaatatct
     8701 tcgaacgcag tatttaatcc agcctggaat tgctcatcga cattactcgt tccccaataa
     8761 gtttgatggc taccactcaa atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg
     8821 agttgtaatt tcccgcgttt gttataagcg agattgtaat agtcggtgaa tttcggctta
     8881 acctgaataa caccatcctg tgtttcgatg ttgtagccat tcattcgact gtatgttgta
     8941 tcagcgaaat taaaataccc actggtcgaa taacggtaac ccactaactg aatattcgtg
     9001 cctgactcat tgagcgattt gttataaaga aaacgcaccg attgtccgtc atgctgactg
     9061 tcatcgggaa gtgtggaatt agcctgcgtc atatccacag acagagcgcc cagtgccccc
     9121 atatttttcc cgataccgaa attaaacgca cgataacgat ccgccagttg cgttccacca
     9181 tatattgtcc agccagccgg aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt
     9241 tcctgttgcg catttccact acggtattct cctgccgtaa tggaataacg tgtatgccct
     9301 tcacgttgca aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt gctgccgtca
     9361 gcctctttaa tcgttacctg caagtcacca ctattacctg cggcatagat atcgttgatg
     9421 gtaaaaggcc ccggtggcac cgtactatta taaatgtcat acccattttg tttaatagtg
     9481 acctgtgcgg taccacgagc aataccgtgg atcaccgggg caaatcctct ttggctatcg
     9541 ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa agttaatacc atcgaaaata
     9601 tcgccctgag tataaccatc acccagcgtc agccgggaac gtaacggaat tatgtctcgc
     9661 tcaagccagg tattgatatg ctgccattta tttttgctac ctgatgaact gtcgctactg
     9721 ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa cccactctgt
     9781 aggtttaaat atgcataatg gctgttaccc ccaattcgat tctgtacact atttccgctg
     9841 aaattataat tgagcaatcc ggcattaata ccgggatccc ataactcagg aggaatataa
     9901 ccacgcgcgc gattactcat aaatgcctga gggatcgtca ggttgagtcg ctgctgaccc
     9961 acatccagtt gcgcagtagc gtcatggatc attgaggtta atggcacgca ggcatcatcc
    10021 gccagcagat tcataccgga gacagaagcc gtattcagcc ccatactggc aagttgcgcg
    10081 cgtgtcaggc agggaacaat cccttgttcg ctgtcgcccg tattaaatgt gacatcacgc
    10141 gttgccatat aaccattatt caaatagata tcaacacgat acgtccctgg cggtaattct
    10201 tgcccatttt caaaacgcga taaatcagcc acagcctggg gatcatccgc taaaaaacgc
    10261 gggttaaaat agagttcggc agatgacaaa ggtgcctgtg cggtaaaagc acaggcaaca
    10321 aagagtcgga caaaaaaacc agccaaacga tgcctacgaa tatgcaagca ttgtgtgttt
    10381 cgctggtaaa gtcttaaatt cagatatgac attattcctc cggtttaaat cacccggcag
    10441 tcaattcttt ttattcaggc gaaaaaatcc ccctgcgtta ttccattacg cccgtcattt
    10501 tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt cctgcatcag
    10561 aaggcaattt aaccgcgctt tcgcccattg gaggcaccaa tgcattttca agaacccgag
    10621 ttccggcatt caactctgtt accgtcaggt aatagggtgt cgggttaatc agcgtcagag
    10681 aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc aacgctaatt
    10741 tagccggacg atagtacagt ttaatgcggc tgataattgc gagctgtagc gtattctcag
    10801 tcaattttga tttatccatt gacggaatcg ctttaacatt catccagaat aaactttccc
    10861 gatcctgtgg taattggtta tttgttgcat caagaatacg caaggtattc tctttttttc
    10921 ctttcatcgc aaacagaggt ggcgtcacga taaaacggcc atcctttaca ccatcggcat
    10981 tttctaccca tgattgaatt aaataggtgc tattttcatc attatttgtc acggcaagtt
    11041 gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc actcccgctt
    11101 cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc aagcagaatg
    11161 ttatttcctg cgatttcctt acattgacgt ttttattact cactgtcctg ttcctgttat
    11221 cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgg gcattggcga
    11281 tgccgccagt aacccgacgc ccggtagcac gatatttggc gatgaaatgt agcgaagtag
    11341 agcctgagta aagccgtttc cagtttactg gaggacgatt aatcggtacg aggtttcctt
    11401 catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct cccacggaaa
    11461 gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca cgttcactca
    11521 ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta tcttccccta
    11581 ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt ttatcaccgg
    11641 cttcaatccg gcaagcttcc gcaataatga cgccctgaaa ttgcatattt ccgccgggca
    11701 gcgtggtatt ccatttattt ccggccagag caaacattgg cagcaacgac gctaacagaa
    11761 ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa
    11821 tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc atccgcatta
    11881 gctgcgccag gggttgcggc accaatggca taataacgcg cctggaacgg aatggtgttg
    11941 gttccgttat tcagggtggt ttgtgcacta aatgacgcac catccaaagc caacgcagtc
    12001 cctgttttat ccaggatctg cacaccaacg tttgttgcgc tacccgcagc agaactttgc
    12061 agagccagta cgttggtatg attcgtgtca attgttgtac ctaagaaggc aacagcagct
    12121 ttgcttgcaa catcggtatc gcaatcattc agttgaatgt taaaaccgac agcagagctg
    12181 gtcgctccat cctggtccag cgatgcggta cgaacctgtc ctaactgaac ggtttgatca
    12241 acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa gtgaacggtc
    12301 ccaccattta ccgtcgtagt atcggccaga gccgctgcgg aactgaggga cagagccgac
    12361 agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa aactatttct
    12421 aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg actgccaaca
    12481 ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca tagaggaaag
    12541 catcgcgcac aaactttttc agtttatttg ttggcttaat gttctatagt tatctttatt
    12601 tgcagttttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa gttgcgtcga
//
LOCUS       DAJOTB010000135        12559 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJOTB010000135 DAJOTB010000000
VERSION     DAJOTB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.135"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            43..1557
                     /gene="caiT"
                     /locus_tag="OHN28_003789"
     CDS             43..1557
                     /gene="caiT"
                     /locus_tag="OHN28_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCQ2299099.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKCLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKY"
     gene            1588..2730
                     /gene="caiA"
                     /locus_tag="OHN28_003790"
     CDS             1588..2730
                     /gene="caiA"
                     /locus_tag="OHN28_003790"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCQ2299100.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2859..4076
                     /gene="caiB"
                     /locus_tag="OHN28_003791"
     CDS             2859..4076
                     /gene="caiB"
                     /locus_tag="OHN28_003791"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCQ2299101.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4149..5702
                     /gene="caiC"
                     /locus_tag="OHN28_003792"
     CDS             4149..5702
                     /gene="caiC"
                     /locus_tag="OHN28_003792"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCQ2299102.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDIYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSVRAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSVNDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5811..6596
                     /gene="caiD"
                     /locus_tag="OHN28_003793"
     CDS             5811..6596
                     /gene="caiD"
                     /locus_tag="OHN28_003793"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCQ2299103.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6602..7192
                     /gene="caiE"
                     /locus_tag="OHN28_003794"
     CDS             6602..7192
                     /gene="caiE"
                     /locus_tag="OHN28_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCQ2299104.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVLAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7311..7706)
                     /gene="caiF"
                     /locus_tag="OHN28_003795"
     CDS             complement(7311..7706)
                     /gene="caiF"
                     /locus_tag="OHN28_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCQ2299105.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7967..11188)
                     /gene="carB"
                     /locus_tag="OHN28_003796"
     CDS             complement(7967..11188)
                     /gene="carB"
                     /locus_tag="OHN28_003796"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCQ2299106.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11206..12354)
                     /gene="carA"
                     /locus_tag="OHN28_003797"
     CDS             complement(11206..12354)
                     /gene="carA"
                     /locus_tag="OHN28_003797"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCQ2299107.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         2932 a         3280 c         3328 g         3019 t
ORIGIN      
        1 ggaacgcaaa ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga
       61 aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt
      121 tggcttacag tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac
      181 gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggctgg
      241 ttctggctgg tgtttggccc gtatgccaaa aagtgtttag gtaacgaacc accagaattt
      301 agtaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc
      361 tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac
      421 tcaacaggcg cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg
      481 tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg
      541 gaagtgattc gccccagctc gaccctggtg ccgctggtag gtgaaaaaca cgccaaaggg
      601 ttgttcggca ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc
      661 agtctgggcc ttgccacgcc gctggtaacc gagtgtatgc aatggttgtt tggcattccg
      721 cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc
      781 gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc
      841 ttcctgatgc tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc
      901 accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc
      961 atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt
     1021 tacgctatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac cgtgcgtgaa
     1081 ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc
     1141 ggtagtaaca ccctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa
     1201 cagtacggtg tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc
     1261 accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc
     1321 tcttataccc tggcgatgtc cacttgccgc gaagtacgtg acggcgaaga acccccgctg
     1381 ctggtgcgca ttggttggtc agttctggtt ggcattatcg gcattgttct gctggcgctc
     1441 ggcggcctga aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc
     1501 aacatcatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa atattaatta
     1561 accccaaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg
     1621 tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag
     1681 tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag ccctggcgga tatgggtatc
     1741 gacagtctgc tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc
     1801 gccgtgtgga tggaactggg tcgtctggga gcgccaactt acgtgctgta tcagttaccg
     1861 ggcgggttta acaccttcct gcgtgaaggt acgcaagagc agatcgacaa gatcatggct
     1921 ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcaggctcc
     1981 gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt
     2041 agtaagtgtt ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac
     2101 ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc
     2161 atcaaagtga ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc
     2221 tttgacgatg tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac
     2281 cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg
     2341 gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag
     2401 gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac
     2461 tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct
     2521 ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc
     2581 gcaatgcagg tgctgggcgg tgtcgggatt gcgggtaatc accgcatcag ccgcttctgg
     2641 cgtgacctgc gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg
     2701 ggccgcgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg
     2761 cgtcttatca ggcctacagg agcacgaatg tcggccggat aaggcgttta cgccgcatcc
     2821 ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta accatgccga
     2881 aattcgggcc gctgtccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt
     2941 ttgccggaca aatgttcgca gaatggggcg cggaagttat ctggatcgaa aacgtcgcct
     3001 gggccgacac cattcgcgtt caaccgaact acccgcaact ttcccgccgc aacctgcacg
     3061 cgctgtcgtt aaatatcttc aaagatgaag gtcgtgaagc gtttctgaaa ttaatggaaa
     3121 ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg
     3181 atgaagtcct gtggcagcac aacccaaaac tggttatcgc tcacctgtcc ggttttggtc
     3241 agtatggcac cgaggagtac accaatcttc cggcctataa cactatcgcc caggccttca
     3301 gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg
     3361 ccgattactt ttctggcctg actgccacca cggcggcgct ggcagcactg cataaagtgc
     3421 gtgaaaccgg caaaggcgaa agcatcgaca tcgccatgta tgaagtgatg ctgcgtatgg
     3481 gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgaccaaag
     3541 ggaaagatcc ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga
     3601 tggaactggt gggcattact caaattgcag agtgctttaa agatattggc ctcgcacatc
     3661 tgctcggcac gccagaaatt ccggaaggca cccagcttat ccaccgtatc gaatgccctt
     3721 acggcccact ggtagaagag aaactcgatg cctggctggc ggcacatacc atcgcagaag
     3781 ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac
     3841 tggaaagcaa tccacagtat gtggctcgcg aatcaatcac tcagtggcaa acgatggatg
     3901 gtcgcacctg caaagggccg aatatcatgc cgaaattcaa aaataacccc ggacaaatct
     3961 ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca
     4021 gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc
     4081 gtggcgtcag tcagcttgcc tgactagtgc ccgctcaata acaaacgaac aaaatggata
     4141 gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg
     4201 acgtttacgg tcataaaacg gcgctgattt gtgaatccag cagcggagtc gttaaccggt
     4261 atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg
     4321 ggattcgcaa aggcgacaag gttgctctac atctcgacaa ctgcccggaa tttatctttt
     4381 gctggttcgg gttggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttgc
     4441 gcgaagaaag cacgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc
     4501 aattttatcc tatgtatcaa cagattcagc aggaagatgc ctctcagttg cggcacattt
     4561 gcctgacaga tatggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa
     4621 atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa
     4681 ttctcttcac ctccggcacc acctcccgac cgaaaggggt ggtgataact cattacaacc
     4741 tgcgcttcgc tggatattac tccgcctggc agtgcgcact gcgtgatgat gacatctacc
     4801 tgacggtaat gcctgcattt catatcgatt gccagtgtac tgcggcaatg gcggcatttt
     4861 ctgccggggc cacctttgtg ctggtcgaga aatacagcgt ccgcgccttc tgggggcagg
     4921 tacagaagta ccgtgccacc gttaccgaat gtattccgat gatgatccgt accctgatgg
     4981 tacaaccacc ttctgtgaat gacaggcaac actgcctgcg ggaagtgatg ttttatctca
     5041 acctgtcaga gcaggaaaaa gacgcttttt gtgaacgctt cggcgtccgc ttgctgacgt
     5101 cttacgggat gacggaaacc attgttggca ttatcggcga tcgccctggc gataaacgac
     5161 gctggccgtc aattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca
     5221 atcgcccgct cccggcaggt gaaatcggtg aaatctgtat taaaggcgta ccagggaaaa
     5281 ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgatg
     5341 gctggctaca taccggcgat accggatacc gcgacgaaga gggctttttt tatttcgtcg
     5401 atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctcgaaa
     5461 atatcatcgc cacccatccc aaaattcagg atatcgtggt tgtgggtatt aaagattcga
     5521 ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag
     5581 aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg
     5641 agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat
     5701 aaatgaaacg gcagggaact accctgtcag ctaataatca cgctctaaaa caatatgcat
     5761 tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat
     5821 cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgt ccaaaagcga
     5881 atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg
     5941 atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaaattcttt tccgcgggct
     6001 gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggaggtt
     6061 ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt catcgccgct gtgaacggct
     6121 atgcctttgg cggcggcttt gaactggcgc tggcggcaga ttttattgtt tgtgccgata
     6181 acgccagctt cgccctaccg gaagccaaac tgggcatcgt tcctgacagc ggcggcgtgc
     6241 tgcgtctgcc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac
     6301 gaatgggcgc agaagaggcg ctgcgttggg gggtagtcaa ccgcgtggtt agccaggcgg
     6361 aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgct ccgctggcga
     6421 ttgcggcgct gaaagagatc taccgcacca ccagcgaaat gccggtagaa gaagcgtatc
     6481 gctatattcg cagcggcgtg ttgaaacact atccatcggt tctgcattcg gaagatgcca
     6541 ttgaagggcc gctggcgttt gccgagaagc gcgatccagt gtggaaagga cgttaacaac
     6601 cgtgagctat tacgcctttg aggggttaat tccggttgtt cacccgacgg cgtttgtcca
     6661 tcccagtgca gtattgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact
     6721 cgcctcactg cgtggtgact acgggcggtt gatcgtgcta gcgggagcca atattcagga
     6781 tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa acggtcatat
     6841 cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcactgg tcgggatgaa
     6901 cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg ctatgagctt
     6961 tgtcaaagcg ggctttcgcg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc
     7021 tgtacgcagc gtgagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca
     7081 ggatcttgtc gggcgctgtc atgcatcgtt acatgaaacg cagccactga ggcaaatgga
     7141 ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg ccgaaacggt gatcttgccg
     7201 gatgcggcgt aaacgcctta tccgacctgg ctggaaagca accgtaggcc ggataagatg
     7261 cgccagcatc gcatccggca accaggctgc gtggtatcaa cgaaatgaca ttaacgacgc
     7321 atactctttg acaacatcat ctgccacttc tgctcgcggt taagttccgt gagcggaacg
     7381 gcaggaatac gcggcgtctt tcttacgccg actagtttat cccggctgtt attgcgcaga
     7441 cgcgcgtaaa tttgctcatc gatatcaacc actttaacca gtcgctgaca ctggcatccc
     7501 cgcccttcca gcttattagg gatcatctta acttcgcagc ttatttctgt gacttccgac
     7561 agaatataag tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg aatagagatc
     7621 tctcttgcta tcacccaccg attttcagcc atcatccatt cggcgattaa caagtagagt
     7681 ggtttttcaa catatccttc acacatacga actccagttt cacaaattaa aagactggct
     7741 atgactcgtt ttgcagcaat taaacaaggc catattaaaa ttgtggatat atcgatagta
     7801 tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa aaatatcgat
     7861 tccccaaaag atttacttaa tgggcaaaaa aaacggtcct catcagagaa ccgtattagt
     7921 tatttgatca aattagcgga tgaaaaatat ccgccatgac acgctattat ttgatctgcg
     7981 cgtgcatttc ctgcaccgaa attacttttt cggtcgcatc ggcattcagc gccatcgcgg
     8041 tggcaaagcc gccgttcagg gtggtgtcgt aatgcacttt atattgcagc gcactgcgac
     8101 ggatcacgcg ggagtcttca atcgcacgac gacctgaggt ggtgttgatg atgtaggtat
     8161 attcgccatt cttaatacgg tcctgaatgt gcggacggcc ttcatgcacc ttgttcacca
     8221 gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgagtc gcatccagct
     8281 cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta tcgccttcgc
     8341 gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc agctgcgctt
     8401 tggcaaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta gagcgcattt
     8461 ctggccctaa cagcgggtca acgcccggga atttattgaa cggcagcacc acttctttca
     8521 ccgagtagta cggcgggata acttctttgg ttacgccctg ctcagccagc gatttgccag
     8581 ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag acgaacggaa
     8641 cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac ttcgttgttt ttcaccgcaa
     8701 actgcacgtt catcaggccg cgcacctgca gttcgaaggc cagtttctgc acctgctggc
     8761 gcatcacatc ctgaatttcc tgacttaagg tgtaggctgg cagtgaacat gcggagtcac
     8821 cggagtgcac acccgcctgc tcaatatgct ccatgatgcc gccaatcagc accatttcgc
     8881 cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaagtgg tccagcaaca
     8941 ctggcgcatc gttagatacg ctgaccgctg tctggaagta gcgacgcagg tcagcttcgt
     9001 catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc accagcgggt
     9061 agccaatctc tttcgctttc tcaaccgcca tttcgatggt ggtaacggtg gcgttcgcag
     9121 gttgtttcag tttcagacgc tcaaccgcat gctggaagcg ttcacggtct tccgcacggt
     9181 cgatggcatc cgggctggta ccgataaccg gtacgccagc agcttccagc gcgcgcgcca
     9241 gtttcagcgg ggtctgaccg ccgtactgga cgataacgcc tttcggcttc tcaatacgca
     9301 caatttccag cacgtcttcc agcgttaccg gctcgaagta gagacggtca gaggtgtcgt
     9361 agtcggtgga gacggtttcc gggttacagt taaccataat ggtttcataa ccatcttcgc
     9421 gcagcgccag cgaggcgtgt acacagcagt agtcgaattc gataccctga ccgatacggt
     9481 tcgggccgcc gccgagcacc atgatttttt cacgatcggt ggacggattc gcttcgcact
     9541 cttcttcata agtggagtac atgtaagcgg tgtcggtggc gaactctgcc gcacaggtat
     9601 ccacgcgctt ataaaccggg tgcaagtcat actggtcgcg cagcttgcgg atttctgctt
     9661 cacgcacgcc cgcgagtttt gccagacgcg catcggcaaa gcctttgcgt ttcagctggc
     9721 gcaggaattc agcgttcagg ccagtgatgc ccacttctgc gactttctct tccagacgca
     9781 ccagctcttc aatctgtacc aggaaccagc ggtcaatgtt ggtcaaatta aacacgccgt
     9841 ctacagacag gcccgcgcgg aaggcatcag cgatatacca gatacgctct gcgcctgcat
     9901 ctttcagttc gcggcggatt ttagtcagcg cttccgggtc gtcgagactc actttcggat
     9961 cgaagccagt tgcgcccact tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg
    10021 tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga cggtcgttag
    10081 caccggcgaa tttctcgaag ttgaagcgag gaattttagt aaccacatag tcgatggacg
    10141 gctcgaagga ggccggagta cgcccaccgg tgatgtcgtt catcagctcg tcgagggtgt
    10201 aacccaccgc cagtttcgcc gccactttgg caatcgggaa accagtggct ttcgacgcca
    10261 gcgcagagga gcgagacacg cgtgggttca tttcgataac aatcaggcga ccgtttttcg
    10321 ggttcaccgc aaactggacg ttggaaccac cggtttcaac gccgatttca cgcagtaccg
    10381 ccatcgaggc gttacgcatg atttgatatt ctttgtcggt cagcgtttgg gctggcgcga
    10441 cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata gagcagacga
    10501 tgatacagtt gtcgttttta tcacgcacca cttccatctc gtactctttc cagccgatca
    10561 gcgactcatc aatcagcagc tctttagtcg gagaaagatc cagaccgcgg gcgcaaattt
    10621 cttcaaactc ttcgcggtta taagcgatac cgccgccgct accgcccatg gtaaaggatg
    10681 ggcgaataat gcacgggaag ccaacttcag cggcaaccgc cagcgcttct tccattgaat
    10741 gagcgatacc ggaacgcgca gtttccagac cgattttctt catcgctacg tcgaaacggc
    10801 ggcggtcttc ggctttatca atcgcatcgg cagtggcacc aatcatggtg acgccgaact
    10861 cttccagcac gccctgacgc tcaagctcca gcgcacagtt cagcgccgtc tgccccccca
    10921 tggttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc acttcccagt
    10981 gaatcggctc gatgtaggtt gcatcagcca tttccgggtc ggtcatgatg gtcgccgggt
    11041 tggagttcac cagaatgacg cggtaaccct cttcacgcag ggctttacac gcttgcgcgc
    11101 cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc agaatcagga
    11161 tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact tagcggtttt
    11221 acggtactgc tcaattaacg cgataaagtg gtcaaacaac ggcgcggcgt cgtgtggacc
    11281 agggctggct tccgggtgtc cctggaagct gaacgccggt ttatcggtac gatgaatgcc
    11341 ctgtaacgta ccgtcgaaca gggatttatg tgtgacacgc aggtttgcag gtaatgttgc
    11401 ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacatttt tctcaacatc
    11461 tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct
    11521 cgccagcgcc agcagctgat ggccgaggca gatgccgaat accggaatat cggtttcgag
    11581 gaatttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag gaccgttgga
    11641 gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt gcgccggaac
    11701 gatggtcaga cgacagcctc tatccaccag catccgcagg atgttgcgct tggcaccaaa
    11761 atcataagcc acgacgtggt acggcagctc gtcttctttt ttcgcttctg gcagaccgcc
    11821 ggtcagcgtc cagctccctt gtgtccagct atagggttct gcggtagtca cttcttttgc
    11881 cagatccatg ccattcaggc cagggaacgc gcgggctttt tctaacgcca gcgccgcatc
    11941 cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt
    12001 cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga
    12061 gaggtcttcg gtattacgaa agttgctggc aatcagcggc aggtcgcgaa tcaccagacc
    12121 ttgggcatgt acctgagaag attcttcatc ggcgtcatta gtgccgacat tgccaatatg
    12181 gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga tttcttgata
    12241 accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg cccctatggc
    12301 ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa tcaaaacacc
    12361 ctccagagaa tattcactca ctttatttgc atattaattc acaatgataa cgtaaatcaa
    12421 tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga tatgggagct
    12481 aaaagtcaac ttaatggcgg gattttttat attattttgt ggctcatgca tgttttacag
    12541 gcattaagcg acaaaaaga
//
LOCUS       DAJOTB010000136        12434 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJOTB010000136 DAJOTB010000000
VERSION     DAJOTB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.136"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            146..538
                     /locus_tag="OHN28_003798"
     CDS             146..538
                     /locus_tag="OHN28_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HCQ2299108.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
     gene            658..1119
                     /locus_tag="OHN28_003799"
     CDS             658..1119
                     /locus_tag="OHN28_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299109.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WSLLVKMDEEMLCFFDSNGRTTMPRKAFSLRTGVTRRQLFPDAIYFIEREF"
     gene            1121..1573
                     /gene="radC"
                     /locus_tag="OHN28_003800"
     CDS             1121..1573
                     /gene="radC"
                     /locus_tag="OHN28_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCQ2299110.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNHNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            1620..2549
                     /locus_tag="OHN28_003801"
     CDS             1620..2549
                     /locus_tag="OHN28_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2299111.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(2536..3150)
                     /locus_tag="OHN28_003802"
     CDS             complement(2536..3150)
                     /locus_tag="OHN28_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299112.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGEPNGIYRPEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWARE
                     DVAWMLQPENINAQINIQNQKYARLSAEDETETESGNASTAKNI"
     gene            3373..3570
                     /locus_tag="OHN28_003803"
     CDS             3373..3570
                     /locus_tag="OHN28_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2299113.1"
                     /translation="MNTNRILRKKEVLHLTGISSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            3572..4039
                     /locus_tag="OHN28_003804"
     CDS             3572..4039
                     /locus_tag="OHN28_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299114.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            4127..4981
                     /locus_tag="OHN28_003805"
     CDS             4127..4981
                     /locus_tag="OHN28_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299115.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
     gene            5582..6955
                     /locus_tag="OHN28_003806"
     CDS             5582..6955
                     /locus_tag="OHN28_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299116.1"
                     /translation="MKIPKWVYDRITEITDYVVGDTQWAVTRRQTVRWFLAHIWLETD
                     DDGWTICTVRDIRSSYASLLGQCEISYQGECFMSTLTDFLPTLSDIEFRAGKASKDPE
                     KRRASAWRFNPLLPVCDESARGKLNLVDLDTGESVSFRALLQGNGKAPGHAIDVEKRQ
                     TALKQTEKDFLAKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGRIEGQYVTY
                     DHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFRQYDVDYRV
                     KSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYGWCGNSKKKALK
                     ILRWWNRYISPLKSALESLLERVHGAHRKSCSSPRNYHRYANEVGLILDLNSEVYQRE
                     KTHHHQYARNKALLAFMICGVEQAYIREVVSMNPGRVCMLDHDGVVATGALSLPDWRG
                     FTMKVKD"
     gene            7133..7757
                     /locus_tag="OHN28_003807"
                     /pseudo
     CDS             7133..7757
                     /locus_tag="OHN28_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            7952..10609
                     /locus_tag="OHN28_003808"
     CDS             7952..10609
                     /locus_tag="OHN28_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HCQ2299117.1"
                     /translation="MDELLTLGCIALFFALVVVPILAIIALSRSTAVRSELATLRRRV
                     EVLEQRGVTEAPATVVTTVPQSVSQMVVPVETSSPAVNIVLSEPVADVTPESVDPWRP
                     QPKPQNEALPTPAVEQSSAFGGVMSSLVRWFMQGNPLAKLGILLLFLGFSFLLRYTVE
                     HSLFPLELRLVATSLFAIALLVVGWRLRHKQLVYALIIQGGATGTLYLTVFGAFWLWQ
                     MLPMTLAFALLVAICAASVGLAIMQKALSLAMLASIGGYLAPLLLSTGGGSHVALFSF
                     YLLLSVGILAISIWQHWRELNLLGLLFTFGIGGLWGLDGYRSEYYLSSQLFLIANTII
                     FGVLSVALSLRAQEKGKQIIDGVLLFASPLVGFGMQYAITRHMEYGPALSALGYGGFY
                     LALAWLALRRYPSLGRPLVLAALALGGAFTTLAIPLALSARWTAMAWALEGLGILWLG
                     VQQQQRRMSYSGTALLVLAVCSALWAQMNGMSALSLVLIFAVLSLSWLAAAWLWRNIQ
                     LQGSWVLLAGGLIFWIIALIGASQLVLKKDSLVLSGVLALMAISVWGWRIVSGRLAWW
                     ELDVSKWLLWPTMLVMLLSQISQHEIFAAGWQNLAWCLALPAAGALLWRDAETLPPRL
                     SRLAHLSLFWMILLALAAELFWFAQDLPWGMAAWGSGLMMAAGGLLIFLVHEAVHRQL
                     WPFRSWPALYASQAMIPVAAVLVVLLVLTNLQDGVVYRQTWLPLVNPLEEGAAFALLG
                     LIVFYRVSQRFFPVQISVCRPWPLIALLALSFWWLNGILLRALAWYGEVPWSAETLWH
                     SRLIQTCFALFWMLAALVVMLRATRRCSRREWLCGSVLLGIVIVKLMLVDSARGGGLA
                     RAVTFIGVAILVLIVGYFSPLPPKAGEEK"
     gene            10606..12001
                     /locus_tag="OHN28_003809"
                     /pseudo
     CDS             10606..12001
                     /locus_tag="OHN28_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
     gene            complement(12188..12325)
                     /locus_tag="OHN28_003810"
     CDS             complement(12188..12325)
                     /locus_tag="OHN28_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299118.1"
                     /translation="MGQHGKSLPSFDAPETLKGRKIEWEYENLTHGNKAYNGYVTAIL
                     N"
BASE COUNT         2888 a         2933 c         3454 g         3159 t
ORIGIN      
        1 attttaccca gtcagtacgt tgtcatgcac ttaccattta ttaggtgatt aaaccccacc
       61 tgtgctggtg cagacataac gtcttatcag aatatattga acaggaaaat cactgatgaa
      121 tattgtccat caggaagggc tagctatgcc cgtcgatcag gaattaataa atatcatatt
      181 aaatgaagcg ggaaatccac cgccgcataa agccaaaatt acagccgtca gtctgttatt
      241 taaagacctg agttacagcg ctgaaaaggg tggttaccat cctgtcgaaa tacggattat
      301 ttcacgcaat gatgaatggt attttgatta cattaccgac ttcagttata tggggacggt
      361 ataccccgaa cttgaaaagg aaattgatat cagctggagt cagcaatatg tctttcttgc
      421 ttatgttggt gacctgcctg ttaacgccgg aagggagctg tttgaactct ggcaatccaa
      481 ctttattcag tatcacggca tgaacatcta caccattacc gttctctggg aaagctaatc
      541 cccctgtact aaccatcact aatttatggg gaattatctg tgacgactga cagagggata
      601 cgtgcgtctc aacagggcga actggactgt ctttgcggtg cttattgcgt ggttaacatg
      661 ctgagctggc tgtatgacgg cagggtgaaa cgacgcccgt taatgaactg gcttattcag
      721 acttatcagc agcgctggcc tttgcatgaa tggctgactg aaggaattga agaagaccgc
      781 ttagactggt taatagctca ggtgttgcag aaagggcatt accgccgcca gttcccggtt
      841 gaaatcacca gaccctttgc agggaagcgg gggttaacag acgggcggct gttcagggaa
      901 atgcagcggt ttcttgacgt cactgaccac tctcgcctga ttatgctcag cgaccagttt
      961 cactggtccc ttctggtcaa aatggacgaa gaaatgctct gcttcttcga cagcaacggg
     1021 cgaaccacca tgccccgtaa agctttctcc ctgcgtactg gcgtaacccg ccgccagctg
     1081 tttccagacg ccatttactt cattgaacgg gagttctgac atgaccatgt ttacccagcg
     1141 tgaaacccgt atccttgacc aggcgcgtga cattattagc cggtactacc agcgtggggt
     1201 tcaactctgt tcccctgatg atgttcgacg gtgcgtgatg gttgagctgg caccgcttga
     1261 acatgaagag tttggcatca tcctactcga taatcataat cagctgctgc accgcgaaat
     1321 cctgttcagg ggtacgctaa actcggtcag cgttcacccc cgtgaagtta ttaagcgtgt
     1381 gctgaaacat aacgccgccg ccgtcatcct tgtccataat caccccagcg gcgagcctga
     1441 acccagccgg tgtgacatcc agttgaccaa aaagcttcag gagctgctgg aaatactgga
     1501 tgtgcggtta cttgatcact ttatcgtggc aggaactgaa acggtgtcga tggctgagcg
     1561 aggactggtg tgatgctgaa agttacttgg tttaatgtac gttattgcgg gttacgcgca
     1621 tgagcaggac agcgttgcgt caggtcgtgc cggtggtgca aaccaccgtg ttcgatgcgt
     1681 atattgtcgg tctggccccc tcgggacggc gaggtatcac cagcctgtta aaccgcagcg
     1741 ccagcatact taaacacggt gcggatgcag cagactatcc gtgggaacaa cttaactacg
     1801 ccgccgttgc caaagtgcgc gcggcactgc tggatgacgg ttacgccgtg tcatcggtca
     1861 acatggcgct gtcagcactc cggggcgtag cgcagacggc ctttaacctc aattacatgg
     1921 atgccgaaac gctggcacgt atccggtcag ttaagcgcgt gagcggcgat gtccagcgca
     1981 agggaagggc gctgggcagg caggaaatcc gggtactgat tcaggctgca aaacagcatc
     2041 cgcaatctgt acggcgctgt cgcgatgtcg ccattgtgct gatgctttgc ggaacgggcc
     2101 tgcgggccgg ggaactggtt aaacttgagc gccgggacta tgacaacggc atcctgacgg
     2161 tgaggcaagg gaaggggcgt aaataccgtg aaattcacgt ggcggatgcg gtggacaagg
     2221 ctattcgtgc gtggctgaaa gttggcgcag acgaggctga cagtgcgttg tttaggcgaa
     2281 ttcagcgcag tggtaaggtc gcaagccaac cgctgactac gaccgggtta acgggtatcc
     2341 tggctgagtt acagcaaacg gcaggtatag cacgttttac cccgcatgat atgcggcgca
     2401 ccttcatcac ccgtttgctg gagcagggcg tggatatcaa taccgtccgc cagttggcag
     2461 gccacagcga catatccacg acgacgcgct atgactgtcg tggtgatact atgaagatca
     2521 gtgccagcaa gcgcatcaga tgtttttagc agttgaggcg ttgccagact cggtttcagt
     2581 ttcatcttca gcactcaacc gcgcgtactt ttgattctgg atgttaattt gtgcattgat
     2641 attttcaggc tgaagcatcc acgcaacatc ctcacgcgcc cattctttac cgtcagagag
     2701 tcgttttaac cggggtttcc atattccata ctcattaagt ttttctgcta ccgaagcata
     2761 tttctcacga tctgactccc ccgtatgaag cttgtcaaaa cgacctttta caagctggta
     2821 aacagtatag atttccagtg ccttagggga cctggccggg actatcatgg ggtgttcctc
     2881 cggcctataa atcccgtttg gctctccctc attgagcagg tcattaaaca tgtggttcac
     2941 gcaggcactc agcacatctg ccgctttctc cgcagcgaat ttatccgggc taaaacccgc
     3001 tcccgtcagt ttccgggcaa tcagtgccag tttttgccgg gtatcccgtc ccagataaag
     3061 cttcttctcc agaaattcaa agacctcgtt actgcttttc cgttcccggt ggcatctggc
     3121 gtcagccttg ttgctttggt taccaatcat agtcacctcc atgacagtgt tttgtacaaa
     3181 atatcaccat tgagcctcag tgaaaattgc ttctttatat gaccgttctg gagacagaaa
     3241 tagatgatct cgaatgacat ccacattaaa gatgccctta acgtgctcaa cgaaggcatc
     3301 gttgagtgaa tccttgcgtt ttttggtggt tgtaaataac ccgctcctga aacagctcaa
     3361 aggagcacaa gcatgaacac caaccgtatt ttgcgtaaaa aagaggtact tcatctgacg
     3421 gggatctcgt cagcgacgtt gtaccgactc atctcgaaag gattctttcc tttgtcaaag
     3481 aggctaacgg gcgacaatgg ccgggcggta ggctggctgg agagtgatat caataactgg
     3541 gttaacagcc gtatgcaggc gggagaatga aatgaaagag ataaaatcga aaaccagttc
     3601 tgcgaaaaaa acgctggttc cttggttcga aactatgggg aaacaggttg gtttatcaca
     3661 agaaacgata tcagcagtgt taaaggagca gcgtttacgg atacgtaagc ttaactacgc
     3721 atctgcatat acagtgtcca atattctcat acaggattgc ggcggggccg atgacatgtg
     3781 gagcaactac gctaaactgg cgcttatcct tgagcgttgt aaatacattc cctgcgggag
     3841 cagggcctgt gcatattgtt ctaccgctca gcaattacat tatcaggggg tggatgtaac
     3901 cggggaatac gctttatatc agttgcctga gttattttta gattttttag aaacaaacga
     3961 caaaaattcc tcttatcatt gggattacaa taagttagtt gagcggcatg aagatgctca
     4021 ggcgaggtca ttcgggtaat agccatttct gtatggtgaa aaactgacat attaccctgc
     4081 cactgcattt tggtggcagg gtaatcaata cagctaggaa tattaaatga acgattctta
     4141 ttacttcaga acatctgacc cggattacat tcgtcacgca ccgtcaatgc ttgagatttt
     4201 aagtaaagca ttccccacta ttattaccca gcaggaaagt gagtatgaac tgcagcgtct
     4261 taaccattta ctaaataaac tgaaaggcag caatcgctgc tacaacatca ttgctcaaaa
     4321 actaaggcag tgcagaaacg gatctccatg caacagtctt atatgcccgc attgccagcg
     4381 ggaacgcatt ctggcgcaac tggctatgtt gcatgtcttg ccggggaatt cagcagaata
     4441 cgtgggagtg gtcctgtttt tcaacaagga tacgcaaact ccgccgccat ggaagaacat
     4501 tggggcgctc agggcgcaaa tcggtcgtta taagcagagg ataagccggg tacttaaccg
     4561 cctggggtac gccggtccgg ccacaggcac cttcagtatg atgcgacata tgcctgatgg
     4621 cccagaagag cgtattttct gggttccaca gttgtgtttg ttcctgccca atgacagtac
     4681 gttgattaag gggctaaaag cgcatatgtc gcgaagtggt ggggcattca ttgatgcatc
     4741 gacgcttaac acaccagtga tcgtgctcag gtataaagat cccgcgagac tgatttcatg
     4801 cgccctgaat ccagtctggc acacagcaga ttacacactg actgataaag acgctcttgt
     4861 taagtcaaga atggagctac tgaaaggccg gacactgtta aaaagtctgc ttaccctgga
     4921 ctcactcgga acaggtgtcg tttctttttc gtttgggcaa cccccaggga aagtgcactg
     4981 atttaccggt aatgcagcaa ctgcaatgca cttttgctgt tgattaaatt gcagtggaac
     5041 gacgaataac agcggcaacc actgcatttt cgttgttgta aaactgcaaa gcttgccagt
     5101 gattttgatg gcgctgatgc actttaacat tccaactgtc tcgattttaa cgcaaccgtc
     5161 tgttttctgc aggggctcac ggatgcctct gcacacccga tccttaccat caactgacga
     5221 gttagcgcat ctgtgagggc tttctgtgtt tctgtcagtg aatattctac acacaatggg
     5281 gggagcggtc agcgagcacc attgccattg agcgcaacgg agagcttggg gacatgcagc
     5341 actacggagg cttcatctcc ttctcatgtc attaaacgat acggcgaacg agcgcagcga
     5401 gtgagggaag gcgtcagcct gaacaggggg aaagatttgc gaccggagtg gagcaaatca
     5461 tgggcggagc ccatcctcgc gactggggtt ttgactttcg aattgcccag cgttgagttt
     5521 ggttaatgta gtgagacgag gatgctgaga ggggaccgtg agtactgaaa aggattatcc
     5581 agtgaagatc cctaagtggg tttatgaccg tattactgaa ataacggatt atgtcgtggg
     5641 ggatacgcaa tgggctgtta cccggcggca gacagtacgg tggttcctgg ctcacatatg
     5701 gctggaaaca gacgatgatg gctggactat atgcactgtc cgggatatcc gtagcagcta
     5761 tgccagtctc ctgggacagt gtgaaattag ttaccagggg gagtgcttta tgtccacgct
     5821 aactgatttc ctgccgacac tttcggatat tgaatttcgt gcaggaaaag cgagcaaaga
     5881 cccggaaaag cgtagagcca gtgcatggcg gttcaacccc ctgctgcctg tgtgtgatga
     5941 gtctgccagg ggtaagttaa atctggttga cttggatact ggtgagtctg ttagttttag
     6001 agctctgctt caggggaacg ggaaagctcc tgggcatgcg atagatgtag agaaacgcca
     6061 gacggcccta aaacaaacgg aaaaggactt cttggccaaa gttgggcggg ggcgcatgag
     6121 tatacagttt gtgaaggaat tgcgtagccg tgagccggat cactattacc gcgcaggaat
     6181 acgcagcctt aaccatctct acaatggcag gattgaggga caatatgtca cttacgacca
     6241 tcattatcgt ctgacgtttg gagggcggta ttatgatcaa gctttccaga atctgcccaa
     6301 tgagttcaaa gctaaatttc ggaccgggtt actcaactat gacatcgaag cgtgcaatct
     6361 ggcctgtctg aaccatctgt tcagacagta tgacgtcgat tatcgtgtga agtcatcgat
     6421 atataaaacc atgatggaac ataccgggct gacccgtaaa cagtgcaaac agatggtcca
     6481 taccaccacc tacagaattg ggcgtgtgac tattggtgtt aatgatggcc ttggggcaaa
     6541 agtgtatgga tggtgcggta acagcaaaaa aaaggcgcta aagattttgc gttggtggaa
     6601 ccgttatatc agtccattga aatctgcgct tgagtcattg ctggaacgtg tgcatggcgc
     6661 acaccgcaag agttgttcat caccgcgtaa ctatcacagg tacgctaatg aagtcggatt
     6721 aatactggat cttaatagtg aggtgtatca gcgtgaaaag actcaccatc atcaatatgc
     6781 gcggaataag gcactgttgg cgtttatgat ctgtggtgtg gagcaggcgt atatccgtga
     6841 ggttgtcagt atgaaccctg gtcgtgtctg catgctggat catgatgggg tagtggcaac
     6901 gggcgcgctt tcactaccgg actggcgtgg tttcacgatg aaagtgaagg actagcttac
     6961 tttcttgaat ttaactatct gcgatctctg ataagggcgt attacgttgt caatcataat
     7021 gggatatgcg cattggaacg atgtgcatat ccagtaagtg atccatgtcc gctttgagct
     7081 gtgagttcaa cgggtcgatg caacgctgtc cctaatcaag ggggacgttg ccatgaaacg
     7141 aagaactcgt attaactaca cgccagagca aaaggtgatt atttgggaca gatataagca
     7201 aggtgattcc ctgcatgata tcccccagaa tgttcgacag atttcactct actatcatgc
     7261 ctacaatcca ccaggccggt ggctaccgtc ctcccgtgcg aaagcggcac cggttagcgt
     7321 tttcgcttga tgaaaaagag gagatatcca gaggactggt agcaaaacgc atcatcaggg
     7381 acatcgctgc taaattatca atagctccct caacgattag ccgcgagatc aggaggcacg
     7441 gcggtgcaaa acaataccgt gcagcaaaag ccgatcctgc tgcgtgggaa aatgctctga
     7501 gaccaaaacc ttgcaagcta attgaaagcc ccacattgtg taaaatcatt gcagagaaga
     7561 tgcatcagga ctggtcgccg gaacaaattg gcggttggct aaaacgctgt tatcccgata
     7621 atcaggaaat gcatgtgtca catgaaatga tttataaaac gctttttata ccaacccggg
     7681 gagctttaaa aaaagagctg cagcaatgcc tcagaagcgg aagagtagtg cgtagatcca
     7741 aaacctcatc acttaaagta aacagagata cattctggtc acgaatgccc ggttgttcgt
     7801 gctttgcgtt ttaatcgcat tttacagttt ctgcctgcgt gcagaggttg atggtatttt
     7861 ctgaaaaagc ggctcgcttt gctaatgcat atggcacaaa tacaacagtt tgtgtcattc
     7921 tgacccatct ttataccgcg acggtggaaa catggatgag ctgctaaccc ttggctgcat
     7981 tgctttattc ttcgcactgg tggtggttcc catcctcgcg attatcgcgc tcagccgcag
     8041 cacggctgtt cgttctgagc tggcgaccct gcgtcgccgg gtggaggtgc ttgagcagcg
     8101 cggtgtgact gaagcacccg ccacagtggt aacaactgtg ccgcagagtg tttcgcagat
     8161 ggttgtgcct gttgaaacgt cttcgcctgc ggttaacatc gtgctgtcgg agcctgtggc
     8221 tgatgtcacg cctgagtccg ttgacccctg gcgtccacaa ccgaagccgc aaaacgaggc
     8281 gctacccaca ccagcggtcg aacaatcttc cgcttttggt ggggtgatgt cgtccctggt
     8341 gcgctggttt atgcagggca acccgctggc aaagctgggt attctgcttc tcttccttgg
     8401 cttctccttc ctgctgcgtt atacggttga acactccctg ttcccgcttg agctacgtct
     8461 ggtggcgact tcgctgtttg cgattgccct gctggtagtg ggctggcgat tgcggcataa
     8521 acagctggtt tatgcgctga tcatacaggg tggagctacg gggacgctct atctcaccgt
     8581 ttttggcgca ttctggctct ggcagatgct gccgatgacg ctggcgtttg cattactggt
     8641 ggcgatttgc gcggcgagcg tgggactggc gattatgcaa aaagcgctca gcctcgcgat
     8701 gctggcaagt atcggtggct atctggcacc gctgttgctt tccaccggcg gcggtagtca
     8761 tgtggcgctc ttctctttct accttctgct ttccgtcggt attctcgcca tcagcatctg
     8821 gcaacactgg cgcgagctta atctgcttgg cctgttattt acctttggta tcggcggtct
     8881 ttggggactc gacggctatc gatcagagta ttacctgagc agtcagcttt tcctgatagc
     8941 caatacgatc atttttggtg tgctgagcgt ggcgctatcg ctacgagcac aggagaaagg
     9001 taagcagatt attgatggcg tgctgttgtt tgcatcgccg ctggtcggtt ttggcatgca
     9061 gtacgccata acccggcata tggaatatgg tccggcgctt agcgcgctgg gttacggcgg
     9121 tttctatctg gcgttagcat ggcttgcatt gcgtcgctat ccctctttgg ggaggccgct
     9181 ggtgctggcg gcgctggcgc tgggtggggc atttaccacg ctcgctatcc cactggcgct
     9241 ttcggcccgc tggacggcga tggcctgggc gctggaaggt ctcggcatcc tctggctggg
     9301 cgtgcaacag cagcagcggc gtatgagcta tagcggcact gcgctgctgg tgctggcagt
     9361 gtgcagcgcg ctgtgggcgc agatgaacgg gatgagcgcg ctttctctgg tgctaatctt
     9421 tgccgtactc agcctcagct ggctggctgc cgcctggctg tggcgcaata ttcagttgca
     9481 gggtagttgg gttctgttgg ctggcggttt gatcttctgg ataatcgcgc tcattggggc
     9541 atcgcaactg gtgctgaaaa aggactcgct ggtgctgtct ggcgtgctgg cgttaatggc
     9601 gatatcggtc tggggctggc ggattgtctc tggtcgtctg gcctggtggg agctggatgt
     9661 cagcaaatgg ttgctgtggc cgacgatgct ggtgatgctg ctgtctcaaa tttcacaaca
     9721 tgagattttt gctgcgggct ggcagaatct ggcctggtgt ctggcgttac ccgctgcggg
     9781 ggcattgctc tggcgtgatg ctgagacgtt gccgccgcgc ctctccagac tggcgcacct
     9841 ttcactcttc tggatgattc tgctcgcgct ggcggcggag ctattctggt ttgcgcaaga
     9901 tctgccatgg ggcatggcgg cgtggggctc cggcctgatg atggccgcgg ggggcctgct
     9961 catctttctc gttcatgagg cggttcatcg tcagctatgg ccattccgaa gctggcccgc
    10021 actctacgct tctcaggcga tgataccggt agctgcggta ttggttgttc tgctagtact
    10081 gacgaatttg caggatggcg tagtttatcg ccagacctgg ctgccgctgg tgaatccact
    10141 ggaggagggg gccgcttttg cgctactggg actcattgta ttttatcgcg tttcgcagcg
    10201 ctttttcccg gtgcagattt cggtatgccg tccctggccg ctcattgcac tgctggcgct
    10261 tagcttctgg tggctgaatg gcatcctgct gcgcgcattg gcctggtacg gcgaagtgcc
    10321 ctggagcgcg gaaacactgt ggcattcgcg gctgattcag acctgttttg ccctgttctg
    10381 gatgctggcg gcattagtgg tgatgctacg tgcgacccgg cgttgctcac gtcgcgaatg
    10441 gctttgtgga tccgtattgt tagggattgt tattgtgaaa ctgatgttgg tagacagtgc
    10501 gcgtggcggc ggcctggcac gcgcggtcac gtttatcggc gtggcaattc tggtgctaat
    10561 cgtcgggtat ttttcaccgt taccgcccaa agcgggagaa gaaaaatgaa atggatgaaa
    10621 gcggtgttat gcagcgtgct actgagtact gcgggcgcgg cggtcagcag cgatgaagtg
    10681 aaggaagcgc caacggacta cgcaacaggc gtcgcgctgg aaaccacggg ggcattcgcc
    10741 ctggtatcgc atttcgctgc cgcaggtggt gtatcagagc actgcatggc cggatttgcg
    10801 cgatgtgcgg gtttttaacc atctggggga tatggtgccg tttgcgctgg tggcgcagaa
    10861 aacccagacc gttacgccag agacagtggc tctgcgtctt ttcccgcttg atatgtcgcc
    10921 ggttccgccg cgtgatgagg gtcaacgtag tggggatgct gttgttttac gttcaaaaaa
    10981 cggtattgaa attcatttgc aaagcgatga tgtcactgcg ctcgggcaga gctatttact
    11041 gacgttgccg gaagagagga aagactctct ttcactgacg caattgcgtc tgaactgggg
    11101 gacgccgacg ggcaactggc aggggaaagc ctcggtttat gtcagccgtg atttacgcta
    11161 ctggcgaccc gtgcaggaag atgcgccgtt aatggatctg acgcgggata gcgatcgtct
    11221 gaaaatggac gccatcagca ccaacctgac attgtcactt gaaggaaatc gctacctgct
    11281 ggtgatcctc aactcgcaaa gcccggcact gacgttaaac agcgtgagcg ctattgctga
    11341 cagtaatgag ccggagtccg aacgtattgt gattggcgct cgggcggata aggtgtcgga
    11401 tgatgaagcc gtctggcgat ggacacagcc gcagcccctg acatcgttgc ggatcgatct
    11461 ggaaaatgaa ggcgttttac cggtggaact ggtctggcgc agcggcgaaa aagaaccctg
    11521 gcagtcactg acaaaaacgg tgttgtatcg cctggacggg aagcgttcag aagatattcg
    11581 tcttcctggc cagttagttg aggctgttcg aatacgtaca attaatgcgc gattgccgga
    11641 ggcattgcca gcattgagcg gtgcgcgcga cagttatcaa ctggttttta acacgcaagg
    11701 caaaggtcct tacatgctgg cgtggggaaa ccgggcggcg aaaaaggccg atgttggact
    11761 cgatatgttg atccctgcgt cgctgcgtaa aacgcaggag atagataatc tgccgtgggc
    11821 tataccgcag gagagcgtaa ctctcggcgg tgaattgcga ctaacggcga cgtcggcagc
    11881 cgaacaacaa agtcagtgga aaacgctgct ggtgtggggg gcgttaatac ttggcgttgc
    11941 cgttctggcg tttatggcat ggcgaatctg gcgagaagtt aaaaaggacg gtgcggcgta
    12001 agccataaaa aagcacagtt gcatagttca cggtgttgat aaactgttac aacagaggcg
    12061 tttaagctga ggcggggaaa taatctattt gtgaataata tgttacttca ctggggcgtg
    12121 agtcatggga atgattttga tacctgcgtt ttttatttta gccatcctga ttatcgctat
    12181 cttcatttca gttaagaatg gccgtcacgt atccgttgta tgccttgttg ccgtgggtga
    12241 gattttcgta ttcccattct attttacggc ccttaagcgt ttccggagca tcaaaacttg
    12301 gtaaagattt accatgttga cccattatgc ggcccctctg gagaccactt tcaggtattt
    12361 tctctactga tatgtacaca tggagagatg tgattgcaaa ttcccatcag cattactacc
    12421 aaaagaatca gaca
//
LOCUS       DAJOTB010000137        12342 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJOTB010000137 DAJOTB010000000
VERSION     DAJOTB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.137"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            30..3932
                     /gene="hrpA"
                     /locus_tag="OHN28_003811"
     CDS             30..3932
                     /gene="hrpA"
                     /locus_tag="OHN28_003811"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCQ2299119.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4205..5005
                     /locus_tag="OHN28_003812"
     CDS             4205..5005
                     /locus_tag="OHN28_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCQ2299120.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            5215..6654
                     /gene="aldA"
                     /locus_tag="OHN28_003813"
     CDS             5215..6654
                     /gene="aldA"
                     /locus_tag="OHN28_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCQ2299121.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(6696..7697)
                     /gene="gap"
                     /locus_tag="OHN28_003814"
     CDS             complement(6696..7697)
                     /gene="gap"
                     /locus_tag="OHN28_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2299122.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            7886..8416
                     /gene="cybB"
                     /locus_tag="OHN28_003815"
     CDS             7886..8416
                     /gene="cybB"
                     /locus_tag="OHN28_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCQ2299123.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            8661..8834
                     /locus_tag="OHN28_003816"
     CDS             8661..8834
                     /locus_tag="OHN28_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299124.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(8906..9055)
                     /gene="hokB"
                     /locus_tag="OHN28_003817"
     CDS             complement(8906..9055)
                     /gene="hokB"
                     /locus_tag="OHN28_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCQ2299125.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            9318..9761
                     /gene="trg"
                     /locus_tag="OHN28_003818"
                     /pseudo
     CDS             9318..9761
                     /gene="trg"
                     /locus_tag="OHN28_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(9799..10722)
                     /locus_tag="OHN28_003819"
     CDS             complement(9799..10722)
                     /locus_tag="OHN28_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299126.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            10939..12282
                     /locus_tag="OHN28_003820"
     CDS             10939..12282
                     /locus_tag="OHN28_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2299127.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         3102 a         2954 c         3286 g         3000 t
ORIGIN      
        1 aatcaggcag cctactgccc actaacgtta tgacagaaca acaaaaattg acctttactg
       61 ccttgcagca gcggctggat tcgttgatgc tgcgtgacag attgcgtttt tctcgccgtc
      121 tgcacggtgt gaagaaggtt aaaaatcctg atgcacaaca ggccattttc caggagatgg
      181 cgaaagagat tgaccatgcg gcagggaaag tcctgctgcg tgaagcggca cgaccggaga
      241 ttacttaccc ggacaatttg ccggttagtc agaaaaagca ggacattctc gaagcgattc
      301 gtgaccacca ggtggtgatc gtcgccgggg aaacgggttc cggtaaaacg actcagctac
      361 cgaaaatctg tatggagctg gggcgcggga ttaaagggct gatcggccat acccagccgc
      421 gtcggctggc ggcgcgaacg gtggcgaacc gtattgcgga agagctaaaa acggagccgg
      481 gcggttgcat cggttacaaa gtgcgtttca gcgatcacgt cagtgataac acgatggtca
      541 agctgatgac cgacggtatc ctgctggcgg agatccagca agaccgcctg ctgatgcagt
      601 acgacaccat cattattgac gaagcgcacg aacgcagcct gaatatcgat tttctgctcg
      661 gctatttgaa agagttgctg ccgcggcgtc ctgacctaaa aatcattatc acttccgcga
      721 ctatcgaccc ggaacgcttt tcgcgccact ttaataatgc gccgattatc gaagtctccg
      781 gtcggaccta tccggtggaa gtgcgctatc gcccgattgt tgaagaagcc gatgataccg
      841 agcgcgatca gttgcaggcg atttttgacg ccgtagacga actgagccag gaaagccctg
      901 gcgacattct gatctttatg agcggcgagc gagaaattcg cgataccgcc gatgcgctga
      961 acaagctgaa cttacgccat accgaaatct tgccgcttta tgcacggctt tccaacagcg
     1021 aacagaaccg cgtgttccag tcgcacagcg ggcgacgcat tgtactggcg accaacgtcg
     1081 cggaaacctc gctgaccgta ccgggtatta aatacgttat cgacccaggt acagcgcgta
     1141 tcagccgcta cagctatcgc accaaagtgc agcgtttgcc gattgagcca atttcgcagg
     1201 cttcagctaa tcagcgtaaa ggccgctgtg gtcgtgtgtc cgaagggatc tgtattcgtc
     1261 tctattccga agacgatttc ctttcgcgcc cggagtttac cgatccggag attctgcgta
     1321 ccaacctggc ctcggttatt ttgcagatga ccgcactggg cttgggcgat atcgccgcat
     1381 tcccgtttgt cgaagcaccg gataaacgca atatccagga tggcgtgcgt ctgctcgaag
     1441 aactgggcgc gatcaccact gatgaacagc aaagtgctta taaactgacg ccgctcggtc
     1501 gtcagctctc gcagttgcct gtcgacccac gtctggcgcg tatggtgctg gaagcgcaaa
     1561 aacatggctg cgtgcgtgag gcgatgatta tcacgtccgc gctctccatt caggatccgc
     1621 gcgagcgtcc gatggataaa cagcaggcag ctgacgaaaa acatcgtcgc ttccacgaca
     1681 aagagtctga cttcctggca tttgtgaatc tgtggaatta tcttggcgag cagcaaaagg
     1741 cgctttcttc aaacgccttc cgtcgcctgt gtcgtaccga ttatctcaac tatctgcgcg
     1801 tgcgcgaatg gcaggatatc tacacccagt tgcgccaggt ggtgaaagaa cttggcattc
     1861 cggttaacag cgaaccggcg gattatcgcg aaattcacat tgcgttgctg accggtttgc
     1921 tttcgcatat cggcatgaaa gatgccgata aacaagaata taccggcgca cgtaacgcgc
     1981 gtttctccat cttccccggt tctggtttat tcaaaaagcc gccgaaatgg gtaatggtgg
     2041 cggaactggt agaaaccagc cgcctgtggg ggcgcattgc tgcgcggatc gacccggaat
     2101 gggtggagcc agttgctcag catttgatta aacgcaccta cagcgaaccg cactgggaac
     2161 gggcgcaggg cgcggtgatg gcaacggaaa aagtcactgt ttatggtttg ccgattgttg
     2221 ccgcgcgcaa ggtcaactac agccagatcg atccggcgtt atgtcgtgaa ctctttattc
     2281 gccacgcgct ggtggaaggt gactggcaga cgcgtcacgc attcttccgc gaaaacctga
     2341 aactacgggc ggaagtagaa gagctggaac acaaaactcg tcgccgcgat attctggtgg
     2401 atgacgaaac gttgtttgag ttctacgacc agcgcatcag tcacgatgtg atctctgcac
     2461 gccatttcga cagttggtgg aaaaaagtca gccgcgaaac gcctgatttg ctcaactttg
     2521 aaaagagcat gttgatcaaa gaaggggcgg aaaaaatcag caagctggat tacccgaact
     2581 tctggcatca gggcaatctc aagctgcgtt tgagttatca gtttgagccg ggggcagatg
     2641 ctgacggcgt gaccgtacat attccgctgc cgctgcttaa ccaggttgag gagagcgggt
     2701 ttgagtggca gatccccggc ctgcgccgtg agctgatcat cgccctgatt aaatcgttgc
     2761 cgaagccggt acgccgtaat tttgtacctg cgccaaacta tgccgaagcg tttttaggcc
     2821 gcgtcacacc gctggaattg ccgttgctcg acagccttga acgagagctt cggcggatga
     2881 ctggcgtcac cgttgaccgc gaagactggc actgggatca ggtgcccgat cacctgaaaa
     2941 tcaccttccg cgtggtggat gacaaaaaca agaagctaaa agaagggcgc tcattacagg
     3001 atctgaaaga cgccctgaaa ggcaaagtgc aggaaacgct gtctgccgtg gcggatgacg
     3061 gtatcgagca gagcggttta catatctgga gttttggtca actgccggaa agctacgagc
     3121 agaagcgcgg caactacaaa gtgaaggctt ggccggcgct ggtggatgag cgtgacagcg
     3181 tagcgatcaa actgtttgat aacccgctgg aacagaagca ggcaatgtgg aacggtcttc
     3241 gccgtttact gctgctaaat attccatcgc cgatcaagta cttgcacgaa aagctgccaa
     3301 acaaagccaa gctggggctg tactttaacc cgtatggcaa agtgctggag ctgatcgatg
     3361 actgtatctc ctgcggtgtg gatcaactga tcgacgccaa tggtggcccg gtctggacgg
     3421 aagaaggctt tgctgcgctg catgaaaaag tccgtgctga actgaacgac acggtggtgg
     3481 atattgcgaa gcaggtcgag caaatcctca ctgcggtgtt caatatcaac aaacgtctga
     3541 aagggcgggt ggatatgacc atggcgctgg ggctttctga cattaaagcg cagatgggcg
     3601 ggctggtgta tcgcggtttt gtcactggta acggcttcaa acgactgggc gacacgctgc
     3661 gatatttgca ggcaattgaa aaacgactgg aaaaactggc ggtcgatcca catcgcgacc
     3721 gtgctcagat gctgaaagtc gaaaacgtcc agcaggcgtg gcagcaatgg atcaacaaac
     3781 taccgcctgc acgtcgtgag gatgaagacg tgaaagaggt ccgctggatg atagaagagc
     3841 tgcgcgttag ttacttcgct caacaacttg gcacgcctta tccgatttcg gataagcgta
     3901 ttttgcaggc gatggagcag attagcggtt aacactgcta tttgcctgat aaatctaaaa
     3961 cccggtaagc atttagcgcc gggtttttta ttaattctaa aaacgataag ggaaaaaatt
     4021 caggagttca gaaaaataca attctctgct gcaagatgaa taatgtttat ctacagcatt
     4081 tccttaaaag atatgtcagg cttgcggagt ggcagttcag gaaatacgac ttcctccttt
     4141 cagagtgctc cgcttctcac tattatctca cgcagtattc ttaagggaac gataaggagg
     4201 aaccatgaat attaccccgt ttccgacgct ttcgccggca accatagatg ccataaatgt
     4261 tatcggacag tggctggcgc aggatgattt ctccggtgag gtgccgtatc aggccgattg
     4321 cgtgatcctt gcaggcaatg cggttatgcc gactatcgat gcggcatgta agattgcccg
     4381 cgatcagcaa attcctttac tgattagtgg tggtatcggt cactcgacaa cttttttgta
     4441 tagcgccatc gcacagcatc cgcactacaa cactatccgc accactggca gagcagaagc
     4501 taccattctg gcggatatcg ctcatcagtt ctggcatatt ccgcatgaaa aaatctggat
     4561 tgaagaccag tcaacaaact gcggtgaaaa cgcacgcttt agcatcgcgt tattgaatca
     4621 ggctgtagaa cgagttcata cggctatcgt tgttcaggac cccaccatgc agcggcgcac
     4681 gatggcgacg ttccgccgta taaccgggga taatcccgat gcaccacgct ggttaagtta
     4741 tcccggattc gttcctcagt taggaaataa cgcagagagt gtaatctttg ttaatccgtt
     4801 gcaaggatta tggccggttg agcgttatct atcgctactc actggcgagc tgccgcgttt
     4861 acgcgatgat agcgatggct acggcccccg cgggcgagat tttatcgttc acgttgattt
     4921 tccggcagaa gtcattcagg catggcaaac gctgaaacat gatgcggtgc tcatcgaggc
     4981 gatggaaagt cgctcgttac gttaaaaatt gcccgtttgt gaaccacttg tttgcaaacg
     5041 ggcatgattt ctgactttta tttctgcctt ttatttctgc ctttaattcc ttttacactt
     5101 gtttttatga agcccttcac agaattgtcc tttcacgatg ccgtctctct gatgattgat
     5161 gttaattaac aataaattta ccaaaaacaa acatgcaaat cacaggagtc gcccatgtca
     5221 gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg tggagacgca
     5281 tggattgatg tggttaaccc tgctacagag gctgtcattt cccgcattcc cgatggtcag
     5341 gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga atgggaagcg
     5401 ttgcctgcta ttgaacgcgc cagttggttg cgcaaaatct ccgccgggat ccgggaacgt
     5461 gccagtgaaa tcagtgcgct gattgtcgaa gaagggggca agatccagca gttggctgaa
     5521 gtcgaagtgg cttttactgc ggactatatc gattatatgg cggagtgggc acggcgttac
     5581 gagggcgaga ttattcaaag cgatcgtcca ggagaaaata tccttctgtt taaacgtgcg
     5641 cttggcgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa
     5701 atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca
     5761 aacaatgcga ttgcattcgc caaaatcgtt gatgaaatag gccttccgcg cggcgtgttt
     5821 aaccttgtac tcgggcgtgg tgaaaacgtt gggcaagaac tggcgggtaa cccaaaggtt
     5881 gcaatggtca gtatgacagg cagcgtctct gcaggtgaaa agattatggc gactgcggcg
     5941 aaaaacatca ccaaagtgtg cctggaattg gggggtaaag caccggctat cgtaatggac
     6001 gatgccgata ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg
     6061 caagtgtgta actgtgcaga acgtgtttat gtacagaaaa gcatttatga tcagttcgtc
     6121 aatcggctgg gtgaagcgat gcaggcggtt caatttggta accctgctga acgcaacgac
     6181 attgcgatgg gaccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg
     6241 cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga
     6301 tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag
     6361 gaaacctttg gtccggttct gccggtggtc gcatttgaca cgctggaaga ggctatctca
     6421 atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc
     6481 gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc
     6541 gaagctatgc aaggcttcca cgctggatgg cgtaaatccg gtattggcgg cgcagatggt
     6601 aaacatggct tgcatgaata tctgcagacc caggtggttt atttacagtc gtagtgagtg
     6661 aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg
     6721 gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac cgttttcacc
     6781 agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac cgaaccgaaa
     6841 tggctgccaa tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta
     6901 ttgtttgtcg cctttttaag cgcgttgttc acctcttcgg cagtcacttt ttttccgaga
     6961 atcgacacca gttcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc
     7021 agtttgccgc tcagttccgg gatcaccagg ccaatggctt ttgccgcccc cgtagtgtgg
     7081 ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg cgggccatcc
     7141 accagtgact gggtgccggt ataggcatga atggtcgtca tcgtgccgac ttctatcccg
     7201 aaactgtcat gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg
     7261 gaaacaatgg tgtcgttgcc atccagagtg tcgtcattga cgttataaac gatggttttc
     7321 atttcaccgg caggggcgga aatcaacacc ttcttcgcgc cagcatcaag atgcgcctgc
     7381 gatttctcgg cggaggtata aaagcctgta cattcgacaa tgatttctgc gcctttcgct
     7441 ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg
     7501 ataagtgaat cttccgtata atcaacgctc caggggaacg gtccgtagtt tgaatcatgt
     7561 ttcagcaggt aggcgagaat ttttggggaa gtgagatcat taatagcgac aacgtctatg
     7621 ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc aaaaccatta
     7681 ataccaactt tactcatggt cttctcctgt caggaacgtt cggatgaaaa ttgatccttt
     7741 ccaggcttag accaggatgg cgggatgggc aatctacatt cttgcagtga aacgtaacgt
     7801 actgaaaacg ggtgaacaat atttaatgaa attttaagaa aagcccgtta tgttaacggc
     7861 aaattatgtt aaagcaggaa atgttatggg aaataaatac tcaaggttac aaatcagcat
     7921 tcactggctg gtctttttgc tggttatcgt agcgtattgc gcaatggagt ttcgtggttt
     7981 cttcccacgt agcgatcggc ccctcatcaa catgattcat gtttcctgtg gcatctcaat
     8041 tctcgtactg atggtggttc gtcttttgtt aaggctgaaa tacccaaccc cgccgattat
     8101 acctaagcca aaaccgatga tgacgggact ggcgcatttg ggccatttgg tgatttatct
     8161 cctgtttatt gcgctgccgg tgattggttt ggtgatgatg tataaccggg gcaacccgtg
     8221 gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgagc gggttgatag
     8281 cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc
     8341 tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc
     8401 acgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta
     8461 caggcgaaga atacgggcct acatcggaag cgcctattat atttatttgt atgacaaata
     8521 aaaccataat ccttgtctat acgtccatct ggcttatctt taatcaattc acccgatctt
     8581 tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga acataagatt
     8641 cgataaatca tggaggttat atgaaaaaac tggcacttat cctggttatg ggaacgcttg
     8701 tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac
     8761 actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa
     8821 catgcactaa ctgaagtgta aaaggggggc catgagaaaa tgaatccgct agtcagatgc
     8881 gcggggaaaa cccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct
     8941 ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg
     9001 tcagaatcgt aatgcagata atgagcagac acaccaccag agggttgtgc ttcatagcct
     9061 ttctccttgc cggatggcgg gtaagaggct aagatctgaa ttgctaggtt cattcgttgg
     9121 cctcggttga tagaaatatc ggtcggggcc ttcgtctttc tgattccagg ttagcctgaa
     9181 aacagaaagt ctcaggcacc cgtgagcatc ctatgtggtt tccttaggga cgaaaataat
     9241 cacctgacga aatcgcgtgc tgctttccag aatttttcgt cattcggttt agccagttta
     9301 atcattcgtt actctcgcaa ggccgtggat ttgccgttgt cgccagcgaa gtacggacac
     9361 tcgcaagccg cagcgcccag gcggcgaaag agattgaagg cttgatcagt gaatcagtca
     9421 ggttaattga cctgggatcg gatgaggtgg caacggcagg gaaaaccatg tgcactattg
     9481 ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat cgccaccgcc tcgggtgaac
     9541 aaagtcgagg cataacgcag gttagccagg cgatttctga aatggataag gtgacgcaac
     9601 agaatgcttc tctggtagaa gaggcctcaa cggcggcggt gtcccttgaa gaacaggcgg
     9661 cacgactaac tgaggcggtg gacgtattcc gtctgaacaa acattctgtg tcggcagaac
     9721 cacgaggagc gggtgaacca gttagttttg ctacggtgtg aaaatgttaa aggagggatc
     9781 gacagatccc ttcacctttc agaacggcat tgattttcga atagcgttaa tcatcaactg
     9841 gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt ccaatcggtt cgcctgcacc
     9901 atgtcccgga acaggcaggg ctaccagcgt ggcatgacgc aggtcgtctt ttacagcgcc
     9961 agaagggaca aaccacacgt aatcgtattc aaccgtaagt tgacgagata gcgaagcaga
    10021 cagcgtttcg atacaacccg aaggaatttt acatccctgg ctctgcacta atgcatctga
    10081 atgctggcgt ggcgcagtgc cttctggtga tacaacgacc ggccattcca gcacccggct
    10141 tagcgttacg ttctcctgaa gtagcgggtg attagggcgg acaaccagct tcagcgattc
    10201 aagaaacagt agttcgtaat taagcccgtt catcagttca ggatctgaca tccgaccaat
    10261 gccgatatcg atttccccgg tttttaaacc cgccagaatc atagggttac tcattgtcgc
    10321 aacttgcaag gtcgtctctt tttgttgctg atgaaactga cctataaccg aaggtaatat
    10381 ccctagtgcc gcagtaggta gcgcaccaac cctgacgaca tcattattaa gaccttcttt
    10441 acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga actctgactg catgcgttaa
    10501 aaattgttcg ccgggtaagg taagttgcgc cccctgacga ccacgctcaa acaagcgagc
    10561 gcccgtcagc tgctccagtt cattcaatgt cttagagagc gcaggttgac tcaaattaag
    10621 ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca gctacgaatg tatgaaggtg
    10681 gcgcaaacgg atgcgctgac taaacagact atttttttcc ataagcgatg ttaaaaacga
    10741 agcggtgtcg ctgacaagtg aagttgtttt attatgataa cttgattgca aaatattatt
    10801 aacaattaaa gcaactatgt tacagcaaaa tggataatat tgatgttttc gcggcgagat
    10861 cacagtttgt aaattcttcc agccggagtg aatgcggtta cctacactcc agattactga
    10921 ccactggagg cagacactat ggcgaacagc atcacggcgg atgagattcg ggaacagttt
    10981 tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc agtacggcac gctgctggaa
    11041 ctggtagctg atgtaaatct ggcggtgctg gaaaacaatc ctcaactgca cgaaaaaatg
    11101 gtaaatgcag acgaactggc gcgactgaat gttgaacgtc atggggcgat tcgcgttggg
    11161 actgcacaag agcttgctac tcttcggcgg atgtttgcca ttatggggat gtacccggtg
    11221 agctattacg atctctcgca ggcaggggtg ccggtacatt cgacagcatt tcggcccatt
    11281 gatgctgctt ctctggcgcg taatcccttc cgcgttttta cctcattact ccgccttgag
    11341 cttatcgaga acgaattatt acgccagaaa gcggcggaga ttctgcgtca gcgcgatatc
    11401 ttcaccccac gttgtcgaca actgttacag gaatatgagc agcggggcgg ttttaacgaa
    11461 acacaggcac aggagtttgt gcaggaagcc ctggaaacgt ttcgctggca ccagtcagca
    11521 acggtagatg aaaaaaccta tcgcgccttg cacaacgaac atcggttgat tgctgatgtg
    11581 gtctgttttc ctggatgcca tatcaaccac ctgacgccac gcacgctgga tattgaccgg
    11641 gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa ttctgatcga agggccgccg
    11701 cgccgcgagg tgccgatttt actacgccag accagcttta aagcactgga agagacggtg
    11761 ttgtttgcgg ggcagaaaca gggcacacat accgcgcgct ttggtgaaat tgagcagcgt
    11821 ggtgtggcat taacgccgaa agggcgacaa ctgtatgatg atcttctgcg gaacgctgga
    11881 accgggcagg ataatctcac tcaccaaatg catttacagg aaaccttccg cacttttcct
    11941 gacagtgagt ttttaatgcg tcagcaaggg ctgggatggt tccggtaccg tctgacgcct
    12001 tcgggtgagg cgcatcgtca ggcgattcat cccggagacg atccacagcc cttaatagaa
    12061 cgtggttggg tagtggcgca acccattacc tatgaagatt tcttgcccgt tagcgcggcg
    12121 gggatcttcc agtcaaatct gggtaatgaa acgcaggcat gcaatcacgg tgatgccagt
    12181 cgcgaagcat ttgagcaggc gttggggtgt ccggttttgg atgagttcca gctttatcag
    12241 gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt aaattctgac catccgcctt
    12301 tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat cc
//
LOCUS       DAJOTB010000138        11999 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJOTB010000138 DAJOTB010000000
VERSION     DAJOTB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.138"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            27..680
                     /gene="ompW"
                     /locus_tag="OHN28_003821"
     CDS             27..680
                     /gene="ompW"
                     /locus_tag="OHN28_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCQ2299128.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(731..1537)
                     /gene="trpA"
                     /locus_tag="OHN28_003822"
     CDS             complement(731..1537)
                     /gene="trpA"
                     /locus_tag="OHN28_003822"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCQ2299129.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPVGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKATTRR"
     gene            complement(1537..2730)
                     /gene="trpB"
                     /locus_tag="OHN28_003823"
     CDS             complement(1537..2730)
                     /gene="trpB"
                     /locus_tag="OHN28_003823"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCQ2299130.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2742..4100)
                     /gene="trpCF"
                     /locus_tag="OHN28_003824"
     CDS             complement(2742..4100)
                     /gene="trpCF"
                     /locus_tag="OHN28_003824"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCQ2299131.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(4104..5699)
                     /gene="trpD"
                     /locus_tag="OHN28_003825"
     CDS             complement(4104..5699)
                     /gene="trpD"
                     /locus_tag="OHN28_003825"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCQ2299132.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5699..7261)
                     /gene="trpE"
                     /locus_tag="OHN28_003826"
     CDS             complement(5699..7261)
                     /gene="trpE"
                     /locus_tag="OHN28_003826"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCQ2299133.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7283..7376)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7353..7397)
                     /gene="trpL"
                     /locus_tag="OHN28_003827"
     CDS             complement(7353..7397)
                     /gene="trpL"
                     /locus_tag="OHN28_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCQ2299134.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7535..8416
                     /gene="yciV"
                     /locus_tag="OHN28_003828"
     CDS             7535..8416
                     /gene="yciV"
                     /locus_tag="OHN28_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCQ2299135.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8413..9033
                     /locus_tag="OHN28_003829"
     CDS             8413..9033
                     /locus_tag="OHN28_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCQ2299136.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            9061..10920
                     /locus_tag="OHN28_003830"
     CDS             9061..10920
                     /locus_tag="OHN28_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCQ2299137.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGISEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGW"
     gene            11133..>11999
                     /gene="rluB"
                     /locus_tag="OHN28_003831"
     CDS             11133..>11999
                     /gene="rluB"
                     /locus_tag="OHN28_003831"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCQ2299138.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNN"
BASE COUNT         2819 a         3052 c         3181 g         2947 t
ORIGIN      
        1 tttataacca taacgacgga gcggatatga aaaagttaac agtggcggct ttggcagtaa
       61 caactcttct ctctggcagt gcctttgcgc atgaagcagg cgaatttttt atgcgtgctg
      121 gttctgcaac cgtacgtcca acagaaggtg ctggtggtac gttaggaagt ttgggtggat
      181 tcagcgtgac caataacact caactgggcc tgacgtttac ttatatggcg accgacaaca
      241 ttggtgtgga attactggcg gcatcgccgt ttcgtcataa agttggcaca gccacaaccg
      301 gggatattgc gacagtccat catttaccgc caacactgat ggcacaatgg tattttggcg
      361 attctggcag taaattccgt ccttacattg gtgcgggtat taactacacc acctttttta
      421 ataatgactt caatgacacc ggtgaaggtc ttggtttatc tgaccttagt ctgaaggatt
      481 catggggcgt ggcggggcaa gtaggcctgg attatctcat caaccgtgac tggttgctga
      541 atatgtcggt ctggtacatg gatatcgata ccgaagtgaa atataaatcc aacggcgcag
      601 tgctgcctgc agggcgttat tctgacaacg tacgccttga cccgtgggta tttatgttct
      661 ctgcaggtta ccgcttctaa ttttttacca tcaataaacc gccagatgtt ttctggcggt
      721 ttgtctcaaa ttaacgtcgt gtagtggctt tcatcggttg tacaaaagct ttcagtgccg
      781 ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacgatg gccgaaccag
      841 aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatcaggg gcggaaatac
      901 caaatccctg caatggaggt gcagcgttgt actctttcag cttcgcaacc agatgattga
      961 ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc acgtgacaat aaataggtgt
     1021 atccacgacc gtaggaggct atctggcgca gcaaatcatc gtcggcattc ggcgggcaaa
     1081 taaagatagg tgcgacatta tgacgcaacg cggcctggcg gaagggcgcg gactcttcaa
     1141 ctggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcgtaaaact
     1201 catcaatgcc tttgttaaac accaggttgg cgtacatcag caggccaaca ggaatggtcg
     1261 gatgtttttg acgaatcagt gccagcatct caaagcactg cgccggggtc acacccgccg
     1321 caaaggcacg cagtgtggcg ttttgaatcg tcgggccatc cgccagtggg tcggagaagg
     1381 ggatgcctaa ctccagcgcg tcagcaccgg cttcaattag cgtatcgata attttcagcg
     1441 actgctcaat gcccggatca ccgagggtga cgaaaggaac gaatgcgcct tctttgcgct
     1501 ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa
     1561 aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag
     1621 ctgctctttt tccggatttt cgcgcatcat tttcagcgca tgggccaggg cgtgggagga
     1681 ttccagcgcc gggatgatcc cttcatgcag gcaaagcgtt ttaaaggctt ccagggcttc
     1741 atcgtcggta atagacacgt aatcagcgcg tccagtgctg ttgagatacg catgttgcgg
     1801 gccgacggac gggaaatcca gcccggcaga aatggagtaa gactcttcaa tttgcccgtc
     1861 ttcggtttgc atcatcggcg ctttcatacc gaaatagatg cccacgcgac catgttttaa
     1921 cggtgcgccg tgctcgccag tttcgatacc gtgaccgcca ggctccacac caatcaggcc
     1981 gacgtcggtt tcgttgatga aatctgcaaa cataccgatg gcattcgaac caccgccaac
     2041 acaggcgata acggcatccg gcaggcgacc ttctctttcc agaatctgcg ctttcgtttc
     2101 ttcgccaatc atccgctgaa actcacgcac aatggtcggg taaggatgcg ggccagctgc
     2161 ggtacccagc atatagtgcg cggtttcata actgccggac cagtcgcgta gcgcctcatt
     2221 acaggcatct ttcagggtcg cggaaccgct atgcaccggg atcacttccg cacccattaa
     2281 gcgcatccgg aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatacg
     2341 gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggcacc
     2401 ggtttcggca ataatttcag ttttacccat ccgcttcgcc agtaaagcct gaccgagtac
     2461 ctggttagtt ttatgcgcgc cgccgtgcag caaatcttcg cgcttcagat acagcgtggt
     2521 gttcgtcccg gctgtaatgt tctggcattt ggtcagcgcg gttggacgcc cggcatagtt
     2581 tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcgc tgacaaaagc
     2641 ttcttccagc tggcgcagag cgggcatcag gatttgtggc acgtacatgc cgccaaactc
     2701 accaaaatag gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc
     2761 tggaaaaccg aggccaaaag acgtgcgtct ttgatacccg gttgcgactc tacagcagaa
     2821 ttaaaatcaa gcccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag
     2881 ccccccgcca gcagaacgtt gccaagcgat tgaccattta atagtgacca gtcgaaacgt
     2941 tgtccgctcc cgccctgacc gttgtcgaat acatatttat cgatgtgctg aaaatcgcgc
     3001 gcgggaagag tttcaccgac acttaaagcc ttccagatgg cgacgtgtgc tggcagagcc
     3061 tcacgcagag tgtcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc
     3121 gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca
     3181 tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt
     3241 gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca
     3301 tcttgcccac gtgtcaggcc acatacttta ttctcaccca gcaacacccg acgcacagcg
     3361 gcgttcaaat cgtcatgggc catcaacgcc gaaccaatca gaaagccgtt agcgaagtgg
     3421 cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac cgtcacgttg
     3481 tgccccagtt tcggtgcaag ctcgcgggta cggttgagat caatcgacaa atcgcgcaga
     3541 tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcgcgctc cagttcctct
     3601 tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc tgcaagctgg
     3661 cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag
     3721 cgcgccagat agatctggta aggatcgata atgaagtctt tacataaaat cggctgcggg
     3781 gcgatttggc tgacgatggg gaggaaatca aagctcccct gaaaatattt ctcatcagtc
     3841 agcactgaaa ttgccgaagc gtaatgttta taaatggcag caatgcgtgc cggatcgaaa
     3901 tcatcacgga tcacgccttt tgacggcgac gcttttttgc actccagaat aaacgccgtg
     3961 cgtgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaatctc attctgaaaa
     4021 ctggccagcg gttgctgctg tttgcgggct tctacccaaa tcgccttgtc tgcgacgatt
     4081 ttcgctaaaa cggtttgcat tatttaccct cgtgccgcca gtgcggtgac tctgtcgtaa
     4141 gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg
     4201 ccatgcaggc gcattaacat ggcgacattc gccgcgacgg ctgcttcgtg agcggcgtcg
     4261 cctttacctt gtaacaagcg tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc
     4321 agttgctcct ggtggtaggg tgtcaggcca aaatcttcag cggtcaattg atagctctta
     4381 atttcgccgt catgcagttc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc
     4441 ccgccgctgt gcaccactgc cgcgcgttga taccccagca cgcgcaaggt ttcggcaatc
     4501 ggcagcacca gctccgggct gtagacacca attaacgcca gcggaggatg cgccgggtta
     4561 attaatggcc ccagcacatt gaacagtgtg cgggttttca gttgctggcg aaccggcatt
     4621 gcatggcgga atccggtgtg atatttcggt gcaaagagga aacagacgcc taattcatcc
     4681 agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc cgccagcaga
     4741 tccgacgagc cggatttact ggagacgcta cggttgccgt gtttcgccac tttcagcccg
     4801 caggccgcgg cgacaaacgc actggcggta gaaatattga tgctgttgct gccgtcaccg
     4861 ccagtaccga cgatatcggc aaacagataa tccgggcgcg ggaacggcgc ggcgttttcc
     4921 agtagcgcgg ttgctgcccc ggcgatctcg ttcgggtgct caccgcgaat tttcatgctc
     4981 accagcgccg ccgccagttg ttccggcttc agctcgccac gtaccaccgc tgaaaacagc
     5041 tggtggcttt cttgttggct aagcgtctgt gcctgataca gtttttccag aatcggttgc
     5101 agcgtgttgg tttgctctag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga
     5161 gcgccctggg tagtaagaat ggattccgga tggaactgga atccacaaac gcgatccgca
     5221 tcgtggcgca ccgccatcac catgccatta aaatgggcgt tgatggttaa accggccgga
     5281 atattactgc caaccagcga gtgataacgc gccactggca gcgggtttgt taatccggca
     5341 aacatcgcct gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc
     5401 tgaccgacat agcccccgta agcttcgaca atcgcctgat gtccgaggca aatgccaata
     5461 attggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc
     5521 acaccggggc caggagaaag catcagcacc ggattgctca tcgtcgccag gcgttcaatt
     5581 aaggtctgcg ccggaatatg gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac
     5641 tgatctgcca ggttgtaagt aaaagagtcg atattatcga gcagcagaat gtcagccatt
     5701 agaacgtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttat
     5761 tacgggtttc gtcggcttcc gactgcggaa cagaatcaag taccacgcca gcaccggctt
     5821 gcacggtggc gactccgttc tctaccagcg ccgagcggat cacaatgcag gtgtcgagat
     5881 cgccatgcgc ggtaaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttctg
     5941 cttcggcaat taactgcata gcgcgtactt tcggcgcacc gcttaacgtc cccatattca
     6001 tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg cagctcacca acaacgcggg
     6061 agaccaggtg catcacgtaa gagtaacggt caactttggt gagatcggcg acgtagcggc
     6121 tgccgggggt gcaaatacgc gccaggtcat tacgggcgag atccaccagc atcagatgtt
     6181 cagaaagctc tttatgatcg gtacgcatct ccagttcgat gcggctgtcg aggtctctat
     6241 ccagtgaacc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg taaatctcaa
     6301 tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca aacagggtga
     6361 aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat
     6421 aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa
     6481 tttctccggc gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc
     6541 tctggttaca ttcacaacgc atatgcggca cggaaaccac cggcagcggc ggcgcggctt
     6601 cggtcagttg ctgacgtagt tcgttaaggc gagcagtgag acgttgtttt tcttcttcat
     6661 tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca
     6721 gcgtttcagc gagataaaaa cagaaatcag ggcagttatt ttccgctgac agttgcggta
     6781 aatcttcaaa tcccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc
     6841 gttcttcctt cggtacattc aacagattct gtaataagcg gaatgcgtca aaaaccgaaa
     6901 gggagcataa gcgggcgtct tcatccagca gtggactgac aggcgggaag cgcagtacgc
     6961 ggcagtttgg tgattgttca ttttccacac ccgcaggcag ggcgttatcc agtagtgcca
     7021 gcagggcttc gccattgccg gaaagtgcct ggattgtgac ggtgtcacct aatgctgtaa
     7081 tgcgcagcgc actgtctacc agtagcaggc tttttaaatc atctttgctg tcgatatctg
     7141 cggattccag cagcagcgtt gccggacgat ccccacacaa ctggtgaaaa agcgcggttg
     7201 ggttgtcgcg ataagcgcct acgcaggtta gcagttcgag agtcggtttt tgtgtttgca
     7261 ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct gggtatctga
     7321 ttgctttacg catggtgaat acactgcccg tttcaggaag tgcgccacca acctttcagt
     7381 acgaaaattg ctttcattgt cgataccctt tttacgtgaa cttgcgtact agttaactag
     7441 ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg
     7501 gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt actcagtgat
     7561 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
     7621 gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc
     7681 tgcaattgca ccagcaagag aggaaatttc acgttcggga ctggcgctga atcttattcc
     7741 tggtgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat
     7801 tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca
     7861 gcgggcgcag ctgattgccg aaagacttga aaaagcacaa atacctgggg cactggaagg
     7921 cgcgcaacaa ctggcgcagg gcggtgcggt gacgcgcggt cattttgcgc gtttccttgt
     7981 ggagtgcgga aaagccagtt caatggcgga tgtctttaaa aagtatctgg cgcgcgggaa
     8041 aaccggatac gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca
     8101 ttctggcggt aaggcggtgc tagctcatcc agggcggtac aatcttagtg ctaaatggct
     8161 gaaaagattg gttgcgcatt ttgccgaaca ccacggcgac gcgatggaag tcgcgcagtg
     8221 ccagcaatcg cccaatgaac gcacccagct ggcgacactt gcgcgtcagc atcatttatg
     8281 ggcatcacaa ggatctgatt ttcatcaacc atgcccgtgg atcgaactgg gtcgtaaact
     8341 ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac
     8401 agagagggaa ttatgagcca gtttttttat attcatcctg ataacccgca gcaacgcctg
     8461 atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc aactgattcc
     8521 ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga tggagcgtat ttgtcgtatt
     8581 cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc tgaactctcg
     8641 acctattcat ttgtcgataa cgttgcgttt cgtttgatga aaaacaacac gccaggcaac
     8701 tataccttca tcctgaaggg gactaaggag gtgccacgcc gtctgttgca ggaaaaacgc
     8761 aaaaccatcg gtatgcgcgt gccgtctaac ccgattgcgc aggcgctgct tgaagcattg
     8821 ggcgaaccga tgctttccac ttcgctaatg ctgccaggca gtgaatttac cgaatccgat
     8881 ccggaagaaa ttaaagaccg tctggaaaaa caggtggatt tgattattca cggcggctat
     8941 ctcgggcaga aaccaacaac ggttattgat ctcaccgatg atacgccggt cgtggtgcgt
     9001 gaaggcgtag gtgatgtgaa gcctttctta taacaaattg cgttccacgg atggaagatt
     9061 atggcaggga tatatcgctg cattttgcta ttgatagttg ggcttttttt ctcttcgttg
     9121 agttatgcga aaaacacgga gatcccttct tatgaagaag ggatctcgct ctttgatgtt
     9181 gaagccactc tgcaaccgaa tggggtgctc gacatcaaag agaacattca ttttcaggcg
     9241 cgaaatcagc agattaagca cggattttat cgtgatttac cgcgactctg gatgcagcct
     9301 gatggggacg ctgcactgct gaactatcat attgttggcg tcacccgtga tggtatttct
     9361 gaaccctggc atcttgactg gcatatcggg ttaatgagta ttgtcgtggg cgataaacaa
     9421 cgtttcttgc ctcaaggcga ctatcattat caaattcatt atcaggttaa aaatgctttc
     9481 ctgcgtgagg gggattctga tctgctaatc tggaacgtga ccggtaacca ctggccgttt
     9541 gaaatatata agaccctatt ttcactcaag ttgcctgata ttgcgggtaa tccatttagc
     9601 gaaatcgatc tctttaccgg agatgagggc gacacatatc gaaatggccg catccttgag
     9661 gacggaagaa ttgaatctcg cgatccgttt tatcgtgaag atttcacggt cctctaccgc
     9721 tggcctcacg ctttacttgg taatgccccg gcaccacaaa cgacgaatat tttcagccat
     9781 cttcttttac cctctacgtc atcgttgtta atttggtttc cgtgcctgtt cctggcgtgt
     9841 ggatggttat atctctggaa gcgcaggccg caatttacgc cggtagatgt gattgaaacc
     9901 gatgtcattc cgccagatta cacgcccggc atgttacgtc tcgatgcgaa gctggtttat
     9961 gacgataaag gtttttgcgc cgatatcgta aatctgattg tgaaaggaaa aattcatctg
    10021 gaagctcatt atgacaagaa ccagcaaatc ctgattcgtg ttaatgaagg cgcgaccaga
    10081 aataatgcgg tattactgcc cgcagagcag ttattactgg aagcgttatt tcgtaaaggc
    10141 gataaggtcg ttcttacggg gagacgcaac agagtcttac gcaaggcatt tttacggatg
    10201 cagaaatttt atctgccgcg taaaaagtct tcgttttacc gacctgatgc gtttttgcaa
    10261 tggggcggga tggcgatatt ggcggtcatt ctctacggta acctgagtcc cgtaggttgg
    10321 gcaggaatga gtctggtagg cgatatgttt attatgatct gctggcttct tcctttctta
    10381 ttttgttccc ttgagctttt gtttgcccgc gatgatgaca agccttgcgt taatcgtgta
    10441 atcatcactt tgtttttacc actgatttgt tcaggcgtgg ccttctattc tctctacatc
    10501 aatgtcggag atgtattctt ttactggtat atgccagcgg gttattttag cgctgttttc
    10561 ctgaccggtt atctcactgg catggggtat atttttctgc caaagtttac ccaaactggg
    10621 cagcaacgtt atgcccacgg tgaagctatc gttaactatc ttgcgcgtaa agaggcagca
    10681 acacacagtg ggcggcggag gaaaggggaa gcacggaaac tggattacgc tttgttaggt
    10741 tgggcggtct cggcaaacct tggcagagaa tgggctgcac gtatcacccc atcactcaca
    10801 gcggctgtcc gcgccccgga aattgcccgt agtggcgttt tgttctcatt acagatgcat
    10861 ctgagtctgg gggccaatac cagtttgttg gggcgaagtt attccggtgg tggctggtaa
    10921 taaagctgat gttaatcgaa acaagcttta accataaaga tagtgtagta tgttgcgcct
    10981 caaagccagg ccagttaaaa cgtcgagtcg tttacttaag gcctgaagag ttcaaacaga
    11041 ctttactatc agtgagtcag atgtaagttc tgactcattg ttgcacaaaa gcatatcaga
    11101 cgcctgggaa ggcgacacca aaaggaagct ctatgagcga aaagctacag aaagtgctgg
    11161 cgcgtgccgg ccacggttct cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
    11221 gtgttgatgg caaaattgct aaactcggcg atcgcgttga aattacccct ggactgaaaa
    11281 tccgtatcga tggtcacctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
    11341 tggcctatta caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccaa
    11401 cggtgtttga ccgtctgcca aagctgcgtg gcgcacgctg gattgccgta ggtcgtctgg
    11461 acgttaacac ctgtggtctg ttgttgttca ccaccgacgg tgaactggca aaccgtttaa
    11521 tgcatccaag ccgtgaagtt gaacgtgaat atgccgtgcg tgtatttggt caggttgacg
    11581 acgcgaaact gcgtgatttg agtcgtggcg tgcagctgga agatggtccg gcagccttta
    11641 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtt actctgaccg
    11701 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc
    11761 tgattcgcgt tcgttacggt gatatcccac tgccaaaagg tctgccacgc ggtggctgga
    11821 cggagctgga cctcgcccag actaactatc tgcgcgaact ggtggaacta ccgccagaaa
    11881 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgtat gaaggcgaat cagattcgtc
    11941 gtgcggtgaa acgtcacagt caggtgagcg gcggtcgtcg ttctggcgga cgtaataat
//
LOCUS       DAJOTB010000139        11963 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJOTB010000139 DAJOTB010000000
VERSION     DAJOTB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.139"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            67..450
                     /locus_tag="OHN28_003832"
     CDS             67..450
                     /locus_tag="OHN28_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299139.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(514..957)
                     /locus_tag="OHN28_003833"
     CDS             complement(514..957)
                     /locus_tag="OHN28_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2299140.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="OHN28_003834"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="OHN28_003834"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCQ2299141.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRNMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="OHN28_003835"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="OHN28_003835"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCQ2299142.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="OHN28_003836"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="OHN28_003836"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCQ2299143.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5372..7096
                     /gene="aceK"
                     /locus_tag="OHN28_003837"
     CDS             5372..7096
                     /gene="aceK"
                     /locus_tag="OHN28_003837"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCQ2299144.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="OHN28_003838"
     CDS             complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="OHN28_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCQ2299145.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8138..11821
                     /gene="metH"
                     /locus_tag="OHN28_003839"
     CDS             8138..11821
                     /gene="metH"
                     /locus_tag="OHN28_003839"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCQ2299146.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2911 a         3066 c         3210 g         2776 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acagtggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaatccaa tcggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggcggtaca ggccgcgcta
     1561 aatatcctct acggcattcc taagcaaact cgcaccgaca aactctctgg cgtttatgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacgga acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag taagtgaata aattttattt atattgttat
     2161 caacaagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttc acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaaggttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcagt taacggcacc atcagctata ccaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgaggca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccgagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgtgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccaggggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggcggtcag gcgttgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagctg tggtagagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggtga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt ggggacaggt
     5161 tatttcgata aagtgacgac cattattcag ggcggcacgt cttcagtcac cgcgctgacc
     5221 ggctccaccg aagaatcgca gttctaagca actgataaag ttgatgattg taggccggat
     5281 aaggcgttca cgccgcatcc ggcaatcaac gcctgatgcg acgatttgcg cgtcttatcc
     5341 ggcaattctc tgctcctgat gagggcgcta aatgccgcgt ggcctggaat tactgattgc
     5401 acaaaccatt ctgcaaggct tcgacgctca gtatggtcga ttcctcgaag tgacttccgg
     5461 ggcgcagcag cgtttcgaac aagccgactg gcacgctgtc cagcaagcga tgaaaaaccg
     5521 tatccatctt tacgatcatc acgtgggtct ggttgtggag caactgcgct gcattactaa
     5581 cggccaaagc acggacgcgg catttttact gcgcgttaaa gagcattaca cccgactgtt
     5641 gccggattac ccgcgcttcg agattgcgga gagctttttt aactccgtgt actgtcggtt
     5701 atttgaccac cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg
     5761 ccgttttcgt accattcccc gcccgctggc gaaagacttt caccccgatc acggctggga
     5821 atctctgctg atgcgcgtta tcagcgacct gccgttgcgt ctgcgctggc agaataaaag
     5881 ccgtgacatt cattacatcg ttcgccatct aacggaaacg ctggggacag acaacctcgc
     5941 ggaaagtcat ttacaggtgg cgaacgaact gttttaccgc aataaagccg cctggctggt
     6001 gggcaaactg atcacgcctt ccggcacatt gccatttttg ctgccgatcc accagacgga
     6061 cgacggcgag ttatttattg atacctgtct gacgacgacc gccgaagcga gcatcgtctt
     6121 tggctttgcg cgttcttatt ttatggttta cgcgccgctg cccgcagcgc tggtcgagtg
     6181 gctacgggaa attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca
     6241 gaagcacgcc aaaaccgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga
     6301 gcagttcatt gaagcgccgg gtattcgtgg aatggtgatg ttggtgttca cgctgccggg
     6361 ctttgaccgg gtatttaaag tgatcaaaga caagttcgcg ccgcagaaag agatgtctgc
     6421 cgctcacgtt cgcgcctgct accaactggt gaaagagcac gatcgcgtgg gccgaatggc
     6481 ggacacccag gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat
     6541 ggcattactg ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac aaattgtgat
     6601 tcgccatctt tatattgagc ggcggatggt gccgctcaat atctggctgg aacaagtgga
     6661 aggtcagcag ttgcgcgatg ccattgaaga atacggtaac gctattcgcc agcttgccgc
     6721 tgctaacatt ttccctggcg acatgctgtt taaaaacttc ggtgtcaccc gtcacgggcg
     6781 tgtggtgttt tatgattacg atgaaatttg ctacatgacg gaagtgaact tccgcgacat
     6841 cccgccgccg cgctacccgg aggacgaact tgccagcgaa ccgtggtaca gcgtctcgcc
     6901 gggcgatgtt ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc
     6961 gctatttgaa gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa
     7021 ccgtatacgt gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag
     7081 cgttcgattc gtataaatca gaggcgaaaa agtcagcgta ttccaccgta tgccagcgtc
     7141 acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc atcggtaata
     7201 cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata
     7261 cacgctgcaa ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt
     7321 ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt tgcgtgggta
     7381 taggcatgta accctttgcg gtgcagtaac ttcgtcacct gttcttcgct cagttgggcc
     7441 agaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac
     7501 attcgcatca ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg attgctttga
     7561 tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg
     7621 tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt
     7681 gcgccgatcg cccagtgacc cagttcgccg acctgacgga caaagccctg ttgctgcatc
     7741 gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgctgtgc cagttccgtg
     7801 agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa
     7861 gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg
     7921 ggaatgggtg cgaccatgac agtctccttt tttctgtatc gtggaaatca ttttcatttt
     7981 tattatcagc gaatgcaata gttgctgaac agatccgatg agttaatgtt gaacaaatct
     8041 catgttgcgt ggtggtcgct tttaccacag atgtgtttat gccagtatgg tttgttgaat
     8101 ttttattaaa tctgggttaa gcgtgtcggg agcaagtgtg agcagcaaag tggaacaact
     8161 gtgtgcgcag ttaaatgaac gtattctggt gctggacggc ggtatgggca ccatgatcca
     8221 gagctatcga ctgaacgaag ccgattttcg tggtgaacgc tttgccgact ggccatgcga
     8281 tctcaaaggc aacaacgacc tgctggtgct cagcaaaccg gaagtgatct ccgccatcca
     8341 caacgcttac tttgaagcgg gtgcggatat catcgaaacc aacaccttca actccacgac
     8401 tattgcgatg gcggattacc agatggaatc cctgtcggcg gaaatcaact ttgcggcggc
     8461 gaaactggcg cgcgcctgtg ccgacgagtg gactgcccgc acgccggaga aaccgcgcta
     8521 cgttgccggt gttctcggcc cgaccaaccg cacggcgtct atttctccgg acgtcaacga
     8581 tccggcattc cgcaacatca cttttgacca gctggtggcg gcttatcgag aatccaccaa
     8641 agcgctggtg gaaggtggcg cggacctgat cctgattgaa accgttttcg acacccttaa
     8701 cgccaaagcg gcggtctttg cggtgaaaac ggagtttgaa gcgctgggcg ttgaactgcc
     8761 gattatgatc tccggcacca tcaccgacgc ctccgggcgc acgctctccg ggcagaccac
     8821 tgaagcgttt tacaactccc tgcgccacgc cgaagcgctg acctttggcc tgaactgtgc
     8881 gctggggcca gacgaactgc gccagtacgt acaggaactg tcgcgtattg cggaatgcta
     8941 tgtcaccgcc cacccgaacg ccgggctgcc caacgccttt ggtgaatacg atctcgacgc
     9001 cgacacgatg gcaaaacaga tacgtgaatg ggcagaagcg ggctttctca atatcgtcgg
     9061 cggctgctgc ggcaccacgc cacaacatat tgcagcgatg agtcgtgcag tagaaggatt
     9121 agcgccgcgc aaactaccgg aaattcccgt agcctgccgt ttgtccggcc tggagccgct
     9181 gaacattggc gaagatagcc tgtttgtgaa cgtgggtgaa cgcaccaacg tcaccggttc
     9241 cgctaagttc aagcgcctga tcaaagaaga gaaatacagc gaggcgctgg atgtggcgcg
     9301 tcagcaggtg gaaaacggtg cgcagattat cgatatcaac atggatgaag ggatgctcga
     9361 tgccgaagcg gcgatggtgc gttttctcaa tctgattgcc ggtgaaccgg atatcgctcg
     9421 tgtgccgata atgatcgact cctcgaaatg ggacgttatt gaaaaaggtc tgaagtgtat
     9481 ccagggcaaa ggcattgtta actctatctc gatgaaagag ggcgtcgacg cctttatcca
     9541 tcacgcgaaa ctgttgcgtc gctacggtgc ggcagtagtg gtaatggctt ttgacgaaca
     9601 gggccaggcc gatacccgcg cacggaaaat cgagatttgc cgtcgggcgt acaaaatcct
     9661 caccgaagag gttggcttcc cggctgaaga tatcatcttc gacccgaaca tcttcgcggt
     9721 cgcaactggc attgaagagc acaacaatta cgcacaggac tttatcggcg cgtgtgaaga
     9781 catcaaacgt gaactgccgc acgcactgat ttccggcggc gtttctaacg tgtcgttctc
     9841 gtttcgtggt aacgatcctg tgcgcgaagc cattcacgcg gtgttcctct actacgctat
     9901 tcgcaatggc atggatatgg ggatcgtcaa cgccgggcaa ctggcgattt acgacgacct
     9961 gcctaccgag ctacgcgacg cggtggagga tgtgatcctc aaccgccgcg acgatggcac
    10021 cgagcgttta ctcgagcttg ccgagaaata tcgcggcagc aaaaccgacg acaccgctaa
    10081 cacccagcag gcggagtggc gctcgtggga agtgaataaa cgtctggaat actcgctggt
    10141 caaaggcatt accgagttta tcgagcagga taccgaagaa gcccgccagc aggctacgcg
    10201 cccgattgaa gtgattgaag gcccgttgat ggacggcatg aacgtggtcg gcgacctgtt
    10261 tggcgagggc aaaatgttcc tgccgcaggt ggtgaaatcg gcgcgcgtaa tgaagcaggc
    10321 ggtggcgtat ctcgagccgt ttattgaagc cagcaaagag cagggtaaaa ccaacggcaa
    10381 gatggtgatc gccacagtga agggcgatgt ccacgacatc ggtaaaaata tcgttggcgt
    10441 ggtgctgcaa tgtaacaact acgaaattgt cgatctcggc gtgatggtgc ctgcggaaaa
    10501 aattctccgt accgctaaag aagtgaatgc tgatttgatt ggcctttcgg ggctgatcac
    10561 gccgtcgctg gacgagatgg ttaacgtggc gaaagagatg gagcgccagg gcttcaccat
    10621 tccgctattg attggcggcg cgaccacctc aaaagcgcac acggcggtga aaatcgagca
    10681 gaactacagc ggcccgacgg tgtatgtgca gaacgcatcg cgcaccgttg gtgtggtggc
    10741 ggcgctgctt tccgacactc agcgcgatga tttcgttgct cgtacccgca aggagtacga
    10801 aaccgtgcgt attcagcacg gacgaaagaa accacgcaca ccaccggtca cgctggaagc
    10861 ggcgcgtgat aacgacttcg cttttgactg gcaggcttac acgccgccgg tggcgcaccg
    10921 tctcggtgtg caggaagtcg aagccagcat cgaaacgctg cgtaattaca tcgactggac
    10981 gccgttcttt atgacctggt cgctggccgg gaagtatccg cgcattctgg aagatgaagt
    11041 ggtaggtgtt gaggcgcagc ggctgtttaa agacgccaac gacatgctgg ataaattaag
    11101 cgccgaaaaa atgctgaacc cgcgtggtgt ggtgggcctg ttcccggcaa accgtgtggg
    11161 cgatgacatt gaaatctacc gtgacgaaac gcgtacccat gtgattaatg tcagccacca
    11221 tctgcgccaa cagaccgaaa aaaccggctt cgctaactac tgtctcgctg acttcgttgc
    11281 gccgaaggtt tccggcaaag cggattacat cggcgcattt gccgtgactg gcgggctgga
    11341 agaggacgca ctggctgatg cctttgaagc acagcatgat gattacaaca aaatcatggt
    11401 gaaagcgctt gccgaccgtc tggcggaagc ctttgcggag tatctccatg agcgtgtgcg
    11461 taaagtctac tggggctatg cgccgaacga gaacctcagc aacgaagagc tgatccgcga
    11521 aaactaccag ggcatccgtc cggcaccggg ctaccctgcc tgcccggaac atacggaaaa
    11581 agccacaatc tgggagctgc tggaagtgga aaaacatacc ggtatgaaac tcacggaatc
    11641 tttcgccatg tggccgggtg catcggtttc gggttggtac ttcagtcatc cagacagcaa
    11701 gtattacgcg gtggcgcaaa ttcagcgcga tcaggttgaa gactatgccc accgtaaagg
    11761 aatgagcgtt accgaagttg agcgttggtt ggcaccgaat ctggggtatg atgccgagta
    11821 agtgataaca ccggggtatt tgccccggtt tttaatgtca taaataatgg attattgaat
    11881 atgataaaaa ttgatgaaat agattttttc gctttcgaag gatgttcaga gtccgaagtt
    11941 actgaactat taggaaaaaa cca
//
LOCUS       DAJOTB010000140        11963 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJOTB010000140 DAJOTB010000000
VERSION     DAJOTB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.140"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..360)
                     /locus_tag="OHN28_003840"
     CDS             complement(<1..360)
                     /locus_tag="OHN28_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2299147.1"
                     /translation="MDEKKLKALAAELAKGLKTEADLNQFSRMLTKLTVETALNAELT
                     DHLGHEKNAPKSGSNTRNGYSSKTVLCDDGEIELNTPRDRENTFEPQLIKKHQTRIPQ
                     MDSQILSLYAKGMTTREI"
     gene            complement(430..759)
                     /locus_tag="OHN28_003841"
     CDS             complement(430..759)
                     /locus_tag="OHN28_003841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299148.1"
                     /translation="MQKAMMDSKELEINGLQISEKTLTQGFNDASNKKLKLSEKEISD
                     ALSLVNRTVLRNTRKTESLLREKIGNKHYRTVQQGVYLVVNEKGKGKYADTDAVRFNI
                     KAFARTL"
     gene            complement(1089..1471)
                     /locus_tag="OHN28_003842"
                     /pseudo
     CDS             complement(1089..1471)
                     /locus_tag="OHN28_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1754..5653)
                     /locus_tag="OHN28_003843"
     CDS             complement(1754..5653)
                     /locus_tag="OHN28_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001746330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2299149.1"
                     /translation="MNRIYSLRYSAVARGFIAVSEFARKCFHKSARRLSFPVFLLAPV
                     LFSAGSLAGTVNNELGYQLFRDFAENKGAFRPGATDIAIYNKQGELVGTLNKATMPDF
                     SSVDSDIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVNRNNAPSLDFHAPRLD
                     KLVTEVAPTAVTAQGAVDGAYLDKERYPVFYRLGSGTQYIKDSSGQLTKIGGAYSWLT
                     GGTVGSLSSYQNGQMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFSTPEGGALEWTFDSSVGTGT
                     LTQGATTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSN
                     GSTWTGAGIIVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVL
                     NQQADNKGQVQAFSSVNIASGRPTVVPTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQ
                     LKAADYGAVLANNADKRATITLDYALRADKVALNGWSESRKGTAGNLYKYNNPYTNTT
                     DYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNAAGYLFHGQLKGNLNV
                     DNRLPEGVTGALVMDGSADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHS
                     VLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTLEATDSTVTLGDSRVFI
                     DKNDGNGTAFTLEEGTSEAVKDTDRSVFNGSAVLNGKTTLDIMNATFNGDISGHTGSH
                     VELLRRSFWNMTKSSTLDSFRSKGGTLSLVTDNWSPKTLTVNTLHASSMNIAMGVSTA
                     DNTGDRIDILNKATGGHNTLDLSSLFDQTVTLKNDLTLASAPVGTSHGYFSFASLNRG
                     FTVYTPDTQVQEKDGRVYWQLKSHAGTTESQVSTDVSDDVTDTTSPVAPNTGSTGSTG
                     ADGIVSEGNNSRSVMPSSDSPAENAGTTVNGSSLFKGADNTSLLKKARAMFAAREFIL
                     SDSADRWTQVVDNSDADGGAWAMAGYSHGGYDDFSLNQSGLNVGFRQSAAGNAWWGMG
                     AEFYRGHSSTDDYRDDFSLWGVHALAGKSFAGGLFVDGMAGYRELSEDYSIQGELSDL
                     SGRAKSHILTAGIRGGWKMHAAPLDMSITPTVSLNGARVNGNRLQGRERSVELHDGDA
                     LWLKAGVEAEKVSGNMTLKAGIWRNITLNDMPGMTLRDDWKARHYDAEKADRYTVSFG
                     LNGKLTEKLSVQAKVNSSIDGYFKTDAEGILGIRYDF"
     gene            complement(5691..6575)
                     /locus_tag="OHN28_003844"
     CDS             complement(5691..6575)
                     /locus_tag="OHN28_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial"
                     /protein_id="HCQ2299150.1"
                     /translation="MRQDAVIDILLIIVFSLSLLICGKVSADTGWQSGGGFQGAVEFG
                     GAITPYDDSLPPWLWKTGGYTGFSNKISELTGSGTRLTVSAPYDLLLLAGKSARAFEG
                     SETSGAGIVPVIQLKGSDGSEIHIQWARGTPGQGLLVLPLSVTGDIQTAGTLAAEVQA
                     FGALAWAEDDGFGARITQTLGNNSADAYVFNGGVPDSSTWRTADGLSFISRMTEGEVS
                     AESVWTQLKQGVPGMPDSPVMADESGYQDLVGEGWFYSGAYALGISSGAVLNLHFSKP
                     VTEQSVWRAGMTVIVRYA"
     gene            complement(6982..7739)
                     /locus_tag="OHN28_003845"
                     /pseudo
     CDS             complement(6982..7739)
                     /locus_tag="OHN28_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(7729..8370)
                     /locus_tag="OHN28_003846"
     CDS             complement(7729..8370)
                     /locus_tag="OHN28_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299151.1"
                     /translation="MPGTDTGDVLCGTDVVKVVSMRQKILLTGTCWYELTGLWHLLSA
                     QGYNVYRVPPGYPCARHGWDLIIVTLSAEPVTGWGRHLSWIRELRAEMSGEMLVLVPE
                     RLEMLKVLRNICPVYSGCMPLSCLERTVRMALNRKTARTGKFRLTSGQRQALKRLSER
                     GRDRPLNLKQSERGLYWHYARLAENVGVRDFRMLLMTGLDREVHKMEDRQYGQ"
     gene            complement(8438..9211)
                     /gene="faeJ"
                     /locus_tag="OHN28_003847"
     CDS             complement(8438..9211)
                     /gene="faeJ"
                     /locus_tag="OHN28_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria accessory protein FaeJ"
                     /protein_id="HCQ2299152.1"
                     /translation="MLNIIHRLKSGMFPALFFLTSASVLAQPLTIPPGHWQEGMAVGV
                     TEFSGTLYVRDVSWQWQPRAVCMSSPDAVQAGLAAGKGGMVSESRRGQNFYILGGHTT
                     SLTTARPGLQPSVTLLQVTPSSPRIEARGELARGQVRYGEITFTLRHLLAWQDNITGG
                     QGWSVVSGEVTPEAEKQVKRQLWQVNGYEWTPDYAGLTARPDAFISGTESLLSQGNGS
                     QHIAGAWVTYLSDVRVNFPGAEEPVKRWQGNLTPVVVYF"
     gene            complement(9228..9992)
                     /gene="faeI"
                     /locus_tag="OHN28_003848"
     CDS             complement(9228..9992)
                     /gene="faeI"
                     /locus_tag="OHN28_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HCQ2299153.1"
                     /translation="MKKVTLFLFAASLLPSCVLAWNTPGEDFSGELKLEGPVTSTRNP
                     WVWKVGQGNEGLEVKQSRGVRDGEQAIPVALPAMTVLLGKTTLTTPAGREGLSPRVSY
                     GKDTEGFSLEWIAPGVAEVTLPVTGDNNVRAGTFVFRMQAAGVLRHVKDGQPAYAGVY
                     DDLNVNGLPGESIAMKASDIPGVLQKMFSGEGPSWLQTMTVSGYSGVSHFSDASLRQV
                     EGAYGAQTVAGSGELRLNGMMPERWRVSLPVSIEYQ"
     gene            complement(10023..10820)
                     /gene="faeH"
                     /locus_tag="OHN28_003849"
     CDS             complement(10023..10820)
                     /gene="faeH"
                     /locus_tag="OHN28_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HCQ2299154.1"
                     /translation="MKIAHHYKFLLSAIISVALFYSAASHADILDGGEIQFNGFVTDE
                     APKWTWQISSPDQTWSVDTADAHTENGQLVFDLRDKGALPFLEGHLYEVAERGGPGFT
                     PFITFSSNGQPFAVKEGSGTTAQRFRASVPVRDPETGNVSGQLSFTLNQGMAVSAGAQ
                     EEGATVPAGMSLVSGQSVTDVQSGTLPQWLKTRLSTLLLMNQGFGNGMSAADNGQVIS
                     QGILADGRVMNLAAAYASAVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            complement(11048..11836)
                     /locus_tag="OHN28_003850"
     CDS             complement(11048..11836)
                     /locus_tag="OHN28_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2299155.1"
                     /translation="MKKTLIALAVAASAAVSGSAMAWTANGTGGSVDLGGTLTPTDVI
                     SPWEVKVGAAVNGLDANIRKGDTIVDIPVKNAIPVLGIRVADATWKAFRGQPGITPKI
                     DYHGAINVNDFSRGEVPLTLDVKGTQDQKIGSLTVNLSAGAEYARYNPSTDKGEKYML
                     YQNGEGELFFGGLGTNSDAISSSAWNLANSLDPEFVANYNDFGVSKISSPYYGNVDNA
                     EFNYSAFYASGVEQGKTIKITLEQPAASDAIAWKASLPVTVSYQ"
BASE COUNT         2972 a         3431 c         2528 g         3032 t
ORIGIN      
        1 gatttcccgc gtagtcatgc ctttggcata cagggacaaa atctggctgt ccatctgggg
       61 gatgcgtgtc tgatgcttct taattagttg cggttcgaag gtattttcac ggtcacgcgg
      121 cgtgttaagt tcgatctcgc catcatcgca caataccgtt tttgacgagt agccattacg
      181 ggtattagag cctgatttag gagcattttt ctcatgaccg agatggtcag tcagttctgc
      241 attgagcgcc gtttcgacgg taagctttgt caacatgcgt gaaaactggt tgaggtcggc
      301 ttcggtttta aggcctttgg ccagttcagc cgcaagggct ttaagtttct tttcgtccat
      361 cgtttgcctg tctccgttgt tggagtgaac atatcaaaaa caggcaatta cacaatttta
      421 attacagtct cataatgtac gtgcgaaggc ttttatatta aatcttaccg catccgtatc
      481 tgcatatttc ccttttcctt tttcatttac taccagataa acaccttgct gaaccgttct
      541 gtaatgttta ttaccaatct tttcccttaa cagactttca gttttcctgg tattacgcag
      601 aacagttctg tttactaagg ataatgcatc actgatttct ttttcagaaa gcttcagttt
      661 tttattagag gcatcattaa aaccctgagt taaggttttt tccgagatct gaagtccatt
      721 gatttccagt tccttcgaat ccatcatggc cttttgcaaa tagtatgttc ctaacgaaca
      781 ggacagcgtt tctccgggtt tctttttatc tcattctgct gacggagagc agtaatctgt
      841 gcggtcagct cagacagctc cgtgcctttt ttgcttttat ccgttatgtc cgccgttttt
      901 ttctgctttt tgggtgtctt ctcacgctgt tttttctgaa ccggaggggg agacgacggc
      961 tgtgccaccg aatcactgat atctaacttt aatatgtcag ataaaaaatc atcctgatgt
     1021 tccgcagctc cggcaaaaga ggggagcgaa aacaataaaa gcaaaaaaac tgattttatt
     1081 gcctttcatc attgtgcctt aaccggagtg gtctggattt tcctgttgta ttcttcaata
     1141 agaagctgtt caatatcgtt acatttgtag tttattttat attcaaataa cggtgtgatt
     1201 gtcttctgta cattcccgga tacgttactt gcagcgtaag attttgcctc attaatgaga
     1261 aaatgcatct ctttcagcgt tttttatatc tctcattaag attagccagt gcgcttgcat
     1321 tttttacata ttgtttcaga tactgatact gttctgaatc caccccctga atatcaatat
     1381 cagcattgac attggtattt attactgatg ttttcttcag atgtttagca tcactacatc
     1441 cctgcaggat aaaaacagac atgcaaagca ttattactga actcatcttt ttcatttttt
     1501 gcccctttca aacaccacct caacgtttaa cgaaaattct aatttattta tccttgagat
     1561 tcaatgcatt acataacacc agttgtacgc atcccacgct ttatcctggc aactgttcac
     1621 atttacgttt tctcagtaac tatcaaagca cagggatcgc ccgcagcctg caatccggtc
     1681 agaccacgac agactacctg aaggggcccg acaggcccct tcagaagaaa aacatcagtg
     1741 ataacagtta ttatcagaag tcataccgga tacccagtat tccttccgca tccgtcttaa
     1801 agtaaccatc aatactgctg tttacttttg cctgcactga cagtttctct gtcagctttc
     1861 cgttgagacc aaacgaaacg gtatagcggt cagccttctc tgcatcataa tgacgggctt
     1921 tccagtcatc acgcagggtc attcccggca tgtcgttcag cgtgatattc cgccagatac
     1981 ctgccttcag cgtcatattt ccggagactt tttctgcctc aaccccggcc ttcagccaca
     2041 gggcgtcgcc atcatgaagc tccacgctcc gctccctgcc ctgcagacga tttccgttca
     2101 cccgggcacc gttcagtgaa acagtcggtg ttatcgacat atcaaggggg gcagcatgca
     2161 tcttccaccc gccacggata cccgctgtca ggatatgact ttttgccctg ccggacaggt
     2221 cactcagctc tccctgaatg gagtagtctt cagacaactc acggtatccg gccataccat
     2281 ccacaaacag accaccggca aaggatttgc ctgccagggc atgcacgccc cacaggctga
     2341 aatcatcccg gtagtcatcc gtgctgctgt gcccccggta aaactctgcc cccatccccc
     2401 accaggcatt accggcagca gactggcgga atcccacatt cagtccgctc tgattgaggc
     2461 tgaagtcgtc atatccacca tgactgtacc ctgccatggc ccatgcaccg ccgtctgcat
     2521 cactgttgtc caccacctgt gtccagcggt cagcactgtc gctgagaatg aactcacggg
     2581 cggcaaacat ggcacgcgct ttcttcagaa gagatgtgtt atccgctccc ttaaacagcg
     2641 aactgccatt caccgtcgtg cccgcattct ctgccggaga atcagaggaa ggcatgacac
     2701 ttctgctgtt atttccttca gacacaatac catcagcccc agtgcttcct gtactccctg
     2761 tattcggtgc aaccggagag gttgtatctg tcacatcatc actgacatcc gttgatacct
     2821 gagattctgt tgttccggca tgacttttca gttgccagta aaccctgccg tctttctcct
     2881 gcacctgtgt gtcaggggta taaacagtaa accccctgtt caggctggca aaggagaagt
     2941 acccgtgaga agttccgacc ggtgcagatg ccagagtcag gtcatttttc agtgtgacag
     3001 tctgatcaaa cagggaggac aggtccagtg tgttatgtcc gcccgttgcc ttattcagga
     3061 tatcaatccg gtcccccgtg ttgtcggctg tgctgacacc catagcgata ttcatactgc
     3121 tggcatgcag tgtgtttacc gtcagcgttt tcggcgacca gttgtctgtc accagggaga
     3181 gcgtcccgcc tttgcttctg aacgaatcca gcgtggagct tttcgtcata ttccagaaac
     3241 tccggcgcaa caactccaca tgcgagccgg tatgaccgct gatatcacca ttaaaggttg
     3301 cgttcatgat atccagcgtc gtcttcccgt tcaagacagc actaccatta aacacgctgc
     3361 ggtctgtatc cttcaccgcc tctgatgtgc cttcttccag cgtaaaggcc gtgccgttac
     3421 cgtcgttttt gtcaataaag acccggctgt cgcccagtgt gaccgtcgaa tctgtggctt
     3481 caagcgtggt gttcagcgtg gcattgcgac caagaccaaa atcagtgttc ttcagtgaca
     3541 gtctgtcaaa ggtaaaactg cggttctccc aatcctcctg actgaatgac gtgggctgag
     3601 tcagaacaga atggtctcca ctggcagcca gtttgtcagc cacagactga gtgttgtatg
     3661 catggataac cggatgcccc tgcagcgtca gacgcccgtt ttcctgggtg aatgtaccgg
     3721 agatatccgc agagccgtcc atcaccagag caccggtaac gccttccggc aggcgattat
     3781 ccacattcag gttgcctttc agctgtccgt ggaacagata ccctgcagca ttgaaacggt
     3841 cagctaccag tttctgtgca tccccctgac tgtgccccac aaactcccag gtggcgttgc
     3901 tgctctggtc cgtggggaaa taaccatagg tgctctgctt caggatgaag taatccgtcg
     3961 tatttgtgta cgggttattg tatttatata aatttccggc agttccttta cgtgattccg
     4021 accagccatt cagtgctact ttgtcagccc gcagggcata atccagcgtg atggtggccc
     4081 gtttatcagc attattcgcc agcacggcgc cataatctgc cgccttcaac tgatgaaacg
     4141 tcagactgtt gccattaaca tccagtgtgc ccccacgata tccccatgag acggtatccg
     4201 gatttacctg ccgctcatca gtcggtacca cggtcggccg gccactggca atattaacac
     4261 tgctgaacgc ctgcacctgt cctttattat ccgcctgctg gttcagtaca acctttccgt
     4321 ccccgacctt cagaccgcct tcattaatac ctgtaccctg taccgtcaga gtaccttcac
     4381 caattttatg caggttatcg cccttaacac cattaacctg ccagtttaca gacaccccgt
     4441 tgtccacaat aataccggca ccggtccagg tacttccgtt agaggttgtt actgtgtaat
     4501 tatcacggaa cgtcagggaa cccgccccct gagaaaccga atccttaagg ttaatctgac
     4561 cattctgccc ctgaaatatc agattcttac cggcattcag atcattcccc tgctgcccgt
     4621 gcatggcata tgtggtggcg ccctgtgtca gggtgcccgt gccgacgcta ctgtcaaatg
     4681 tccactccag cgcccctcct tccggtgtac tgaatgtaac cggggcatca ttgtcctcat
     4741 taaatttttg cccgataaaa tccagtggaa taacagccca gttatttccc ctgccccccg
     4801 ctccgttccc cgcagtgaga acaccaacca gcacccattt attctgaaca gtatcaaaag
     4861 caaataaagg cgaaccactg tcaccggcct cgccataaat gggcattgca ccattaagtt
     4921 tgtaatcaaa aaccagacct gaactggtgc taatcatttg accattctga taagatgaca
     4981 aacttccgac agtcccgccg gtcagccagg agtaggctcc accaattttt gtcagttgcc
     5041 cgctactgtc cttgatatac tgagtgcctg agcccagacg ataaaaaaca ggataacgct
     5101 ccttatccag atatgcacca tccacagctc cctgcgccgt caccgcagtc ggagcaacct
     5161 ccgtcaccag tttatccagc ctgggagcat gaaaatccag tgacggtgca ttattccggt
     5221 tcacgatatt gtaacggttt tcaccatcac caaagctgac gtttgtatat cccccgttat
     5281 gtttcacgct ggcgatatac tgaggattaa tcagtgtcgc cacgccaata tccgaatcca
     5341 cagagctgaa atccggcatg gtagctttat tcagcgtgcc tacaagttct ccctgcttat
     5401 tataaattgc aatgtctgtg gcccccggtc ggaacgcacc tttattttca gcaaaatcac
     5461 gaaataactg atatccgagt tcattattga ccgttcccgc aagacttcct gcagagaaaa
     5521 gtacaggggc cagtaagaaa acaggaaaac tcagacgtct ggcagactta tgaaaacatt
     5581 tcctggcaaa ctcagagacg gcaataaaac ccctggccac agcactgtag cgaagagaat
     5641 aaattctatt catttttaat taatcctgaa aaaaataaaa tcagcactac tcaggcataa
     5701 cggacaatca ccgtcatgcc tgcccgccag actgactgct ctgtcactgg tttactgaaa
     5761 tgaagattaa gaactgcacc ggaagaaatc ccgagtgcat atgccccgga ataaaaccag
     5821 ccttcaccta ccaggtcctg ataaccgctt tcatcagcca tcaccggtga atcaggcata
     5881 cccggaacgc cctgtttcag ttgtgtccag acactttctg ctgacacttc accttccgtc
     5941 atccgggaaa tgaaactcag cccgtcggcg gtgcgccacg tactgctgtc gggtacgccg
     6001 ccattaaaca cataagcatc agcactgtta ttccccagtg tctgggtgat tcgggcacca
     6061 aaaccatcat cttccgccca cgcaagtgcc ccgaaagcct gaacttctgc tgccagtgtg
     6121 cctgctgtct gaatgtcccc ggtgacgctc agtggcagaa caagcagccc ctgtcccggc
     6181 gtgcctctgg cccactgaat atggatttcg cttccgtcac ttcccttcag ctgaataaca
     6241 ggaacaatac cagcgccgga tgtctcactg ccctcaaaag cacgggctga tttaccggca
     6301 agcagcagaa ggtcataagg tgcactgacc gtcagacggg ttccgctacc ggtcagctca
     6361 ctgattttgt tgctgaaccc ggtatatccg ccggttttcc acaaccaggg cggaagtgaa
     6421 tcatcataag gcgtgatggc ccccccgaat tccacggctc cctgaaagcc cccaccggac
     6481 tgccatccgg tatcagcaga aacttttccg caaattaata aagaaagaga aaagacaata
     6541 ataagtaaaa tatcaattac agcatcctga cgcacatcca ctcccctctg agactccttc
     6601 acctgtatta ttcactcgtg gtgaaatttc ttttattttt ctgtaaacct cactgaagtc
     6661 atcttccacc attacatgac cttcaattcc cctgatatga agttcacaaa aaataccttt
     6721 ctgaacgacg aaactgatat cttttatatt caccagaaga tttcgcccct gcctgctgac
     6781 aaagtgaata aacacgtctg ccactcaaca caaaacatga acaaaagaat aaaaaacaaa
     6841 aaaagtcgaa gttaagaaga aaaactctgc cggagtctga aaaatgcgat gtggcacatc
     6901 atttgtcaga aataaaatga aattacttaa atgtatttaa ttttattttt caggagtgac
     6961 ttactgatta cacagctggc gttacctgta ctcacaacaa aatgtatcca ctttttccgg
     7021 tctgccgaaa taaaacccct gcagataatc cactcccaga gagcacagac agttcacctg
     7081 cgcctttgtt tccacacctt ctgccaccgt atcaataccc agctttcctg ccagcccgac
     7141 aacactttcc acgatatgca gggatgtctt ttcactcaga atgtcctgtg tgaagctcct
     7201 gtccattttt atataactga ccggaaagtg cttcaggtac gacagtacgg aacatccggt
     7261 tccgaaatca tccagcgcaa atgccactcc ggcatcacac agccggctca gtttctcttc
     7321 ggtctgtctg tccatgttaa acgaccgctg ctccgtcagc tccagaacca gatgtatgct
     7381 gtcatgccct gccagcgcca gacacatctg agtgaactca cgctcagcca gcaaggccgg
     7441 cgtcacgttc acggcaagcc ggaagtcacg cggcagcatg gctttaacct ccgaaagccc
     7501 tgccaccgca cggttcagga ttttttccgt cagaggcaac aacagaccgg cagattccac
     7561 ccggttatga aatatgccgg cggaatgatt tcgcctgcag gcatgtgcca gcgaaccaga
     7621 agctctgcac cggacacagt aaggtctgat gcagaaacaa ccggctggag atacggttca
     7681 aactccccgt tactgagcgc acgcatcagt ctgtctgcgg atacaaaatc actgtccata
     7741 ctgtcggtct tccattttat gaacctcccg gtccagcccg gtcatcaaca gcatcctgaa
     7801 atccctgacg cccacattct ctgccagccg ggcataatgc cagtacagcc cccgttcact
     7861 ctgtttcaga ttcaggggac ggtctcttcc tctctcagaa aggcgcttca gtgcctgtcg
     7921 ttgtccggac gtcaggcgga atttaccggt acgggctgtc tttctgttca atgccatgcg
     7981 gacagttctt tccaggcacg acagcggcat gcatccgctg tacaccgggc agatatttcg
     8041 caacaccttc agcatttcca gcctttccgg caccagaacc agcatttcac cggacatttc
     8101 tgccctcagt tcacgtatcc aggacagatg acgcccccag cccgtaacag gctctgcact
     8161 cagcgtcaca ataatcaggt cccagccatg ccgggcacag ggataccccg gaggtacacg
     8221 gtaaacgttg tagccctgtg cggataacag gtgccacagc cctgtcagct cataccagca
     8281 ggttccggtc agaaggattt tttgcctcat acttacgacc ttcaccacat cagtaccgca
     8341 cagaacatca ccagtgtcag taccaggcaa gccatgcagg tggttatcac acaagccccc
     8401 caccacatcg gtattaacac ttttcgttgc cgggccatca gaaatacacc accaccggag
     8461 tcagattacc ctgccagcgt tttaccggct cctcagcccc ggggaaattc acccgcacat
     8521 cactcaggta cgtcacccag gcaccggcaa tatgctggct gccattcccc tgtgacaaca
     8581 gcgactcagt tcctgaaata aacgcatcag gacgtgcggt taacccggca taatccgggg
     8641 tccattcata tccgttcacc tgccataact ggcgtttcac ctgtttttcg gcctccggcg
     8701 tcacctctcc gctgaccacg ctccagccct gaccgccagt aatattgtcc tgccatgcca
     8761 gaagatggcg cagcgtgaac gtgatttccc cgtaacgcac ctgtccccgg gcaagttctc
     8821 cccgggcctc aatacggggg gatgatggtg tcacctgaag caatgtcacc gacggctgca
     8881 gccccggacg ggcagttgtc agcgatgtgg tatgcccgcc aagaatataa aaattctgcc
     8941 ctctccggct ttcactgacc atgccacctt tacccgctgc caggccagcc tgcacagcat
     9001 caggagagct catacacaca gcgcggggct gccactgcca ggacacatcg cggacataca
     9061 gcgtgccgct gaattccgtc acgcccacag ccattccctc ctgccagtgg cccggcggaa
     9121 tagtcagggg ttgtgccagc acactggctg aagtcagaaa aaacagagcc ggaaacatac
     9181 cggacttcag acgatgaata atattcagca ccgggccgtc ctcctgctta ctggtactca
     9241 atacttaccg gcagtgacac ccgccagcgt tccggcatca tgccgttcag acgtaattca
     9301 ccactgcctg ccacagtctg tgcaccatac gcaccttcaa cctgacgcag ggaggcatca
     9361 ctgaaatgac ttactcccga ataaccactg actgtcattg tctgcagcca ggacggacct
     9421 tcaccactga acatcttctg cagaacaccc ggaatatcag aagccttcat agctatgctt
     9481 tcaccaggca gaccattcac attcaggtcg tcatacacac cggcatacgc cggctgcccg
     9541 tccttcacat ggcgcaacac ccccgcagcc tgcattctga aaacaaatgt gcctgcccgg
     9601 acgttattat ctccggtgac aggcagcgtc acctccgcca cgcctggtgc tatccattca
     9661 agcgaaaatc cctcagtatc ctttccgtaa ctgacacggg gagaaagccc ctcacgtcct
     9721 gccggtgtgg tcagggtggt ttttcccagt aaaacggtca tcgccggcag tgcgaccgga
     9781 attgcctgct caccatcacg aacaccgcgg ctctgtttaa cctccaggcc ttcatttccc
     9841 tgtccgactt tccataccca cgggttacgg gtgctggtca ccgggccttc cagcttaagc
     9901 tctccgctga aatcttctcc cggcgtgttc caggccagta cacaggaagg caggaggctg
     9961 gcagcaaaca gaaacaacgt cacctttttc atttctcttc tctccttata tccctgcaac
    10021 catcactgta ccgtaaccgt cacattcagc cccgcctgcc atgctgcagg cgtgccctct
    10081 gccggtaacc gcagttcaaa atccgacact gctgaggcat atgccgcagc cagattcatc
    10141 acccgaccgt cagccagtat cccctgagag atgacctgac cattgtctgc agcactcatg
    10201 ccattgccaa acccctggtt catcagaagc aatgtcgaaa gacgggtttt aagccactgt
    10261 ggcagcgttc ctgactgtac gtcagtcacg ctctgtccgc tgaccagcga cattcctgcc
    10321 ggcactgttg ccccctcttc ctgtgcaccg gcactgactg ccatcccctg attcagggtg
    10381 aaagaaagct gccccgacac atttcccgtt tccgggtcac gcaccgggac agaggcgcga
    10441 aaacgttgcg ccgtagtgcc actgccttcc tttacggcga aaggctgtcc gttactgctg
    10501 aaggtaataa atggcgtgaa tccggggcct ccgcgctcag ccacctcata caaatgtcct
    10561 tccagaaacg gcagcgcccc tttgtcacgc aaatcaaaaa caagctgtcc gttctctgta
    10621 tgtgcatctg cagtatccac tgaccaggtc tggtcaggtg aacttatctg ccaggtccat
    10681 ttcggtgctt cgtcagtgac aaaaccatta aactgaattt cgccaccatc aagaatatct
    10741 gcatgtgacg ctgctgaata aaacagagcc accgaaataa tggcagaaag aaggaattta
    10801 taatgatgcg ctattttcat tgttatttac tctgctctgt tccggagtac ataaaaataa
    10861 aaaccagaac attcaaacgt gttcccactg aaaacaatac agaaccgaca tgcgtcacaa
    10921 cgcattccga cagaaaaaaa cacctgctcc aacatcccgg ttccgtatat tcaggtcacc
    10981 ggaaacagtg acctgaattt ttagtcagca gtaaacgggg gagggtttac tgccagattc
    11041 tgctgaatta ctggtaagaa acagtaactg gcagagaagc tttccatgca atagcatctg
    11101 atgcagcagg ctgctcaaga gtgatcttga tagttttgcc ttgctcaaca ccggaagcat
    11161 agaatgcact gtaattaaat tcggcattat ctacattgcc ataatatgga ctcgatattt
    11221 tactaacacc gaaatcgtta tagttagcaa caaactctgg atctagagag ttagctaaat
    11281 tccaagcact agaggaaatg gcatcgctat tagtacctaa gccaccaaag aataattcgc
    11341 cttcaccatt ctgatagagc atatactttt cacctttatc agtgcttggg ttatagcgag
    11401 catattccgc acctgctgat aaattaacag ttaaagagcc aattttctga tcttgcgtac
    11461 ctttaacatc aagagtcagc ggaacttcac cacgagaaaa atcatttaca ttaattgcac
    11521 catgataatc aattttagga gtaatccccg gttgccccct gaaagctttc catgtagcgt
    11581 ctgcaacacg aatacccaga acggggattg cattcttcac agggatgtca actatcgtat
    11641 cgcctttgcg gatattagca tccaggccat ttaccgctgc gcctacttta acttcccacg
    11701 ggctaatcac atctgtcgga gtcagggtac cgcccaggtc aacagaacca ccagtgccgt
    11761 ttgcagtcca cgccatagca gaaccagata ctgcagcaga ggcagccaca gccagtgcaa
    11821 tcagagtctt tttcataaaa taatttcctt gatatttcat aaataataaa ctgaaatcgg
    11881 taatccttat ttttccggaa ttaccgtaaa actatcccct gcggattcat ttaattaacc
    11941 gcatttcatt aaacactgtg ttt
//
LOCUS       DAJOTB010000141        11799 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJOTB010000141 DAJOTB010000000
VERSION     DAJOTB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.141"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..682)
                     /locus_tag="OHN28_003851"
     CDS             complement(<1..682)
                     /locus_tag="OHN28_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2299156.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIPSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRK"
     gene            787..1017
                     /locus_tag="OHN28_003852"
     CDS             787..1017
                     /locus_tag="OHN28_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2299157.1"
                     /translation="MNSAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            1087..1626
                     /locus_tag="OHN28_003853"
     CDS             1087..1626
                     /locus_tag="OHN28_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HCQ2299158.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMTRLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1623..2642
                     /locus_tag="OHN28_003854"
     CDS             1623..2642
                     /locus_tag="OHN28_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCQ2299159.1"
                     /translation="MTGSEMAKAGLREQSRLSGANRNALIAEGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVDL"
     gene            2639..3340
                     /locus_tag="OHN28_003855"
     CDS             2639..3340
                     /locus_tag="OHN28_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HCQ2299160.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNGIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTSL
                     YQNMRANALTDTELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGVMK"
     gene            3591..7142
                     /locus_tag="OHN28_003856"
     CDS             3591..7142
                     /locus_tag="OHN28_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HCQ2299161.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQTYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDNYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNVHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVTDSGVSTADSSFT
                     LDGDSSPTISADSQSTYPIVLSLKDSNGKALTGLADDIEMSVEFTADSNSARQRETVT
                     APSLGAVEEISAGVYRSVLAAGSQAGTVRVTAKVQGKTFTLSIKQTAATEPDSEVSAV
                     LTAAPAEQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYS
                     ATVTSKRAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRN
                     MVTVSLADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVI
                     TAHAGSQMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNAS
                     VNGQKIQLIASSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGS
                     VKLTFVPDVASAELNMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTG
                     ASLTSSNLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVL
                     EITSSASSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTT
                     DENGQATVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPGYET
                     NDGSPVGIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEF
                     STYESGYHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
     gene            complement(7179..8222)
                     /locus_tag="OHN28_003857"
     CDS             complement(7179..8222)
                     /locus_tag="OHN28_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2299162.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            8572..8673
                     /locus_tag="OHN28_003858"
     CDS             8572..8673
                     /locus_tag="OHN28_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299163.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            8670..9125
                     /gene="ybcN"
                     /locus_tag="OHN28_003859"
     CDS             8670..9125
                     /gene="ybcN"
                     /locus_tag="OHN28_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HCQ2299164.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            9125..9295
                     /locus_tag="OHN28_003860"
     CDS             9125..9295
                     /locus_tag="OHN28_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299165.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            9288..9578
                     /locus_tag="OHN28_003861"
     CDS             9288..9578
                     /locus_tag="OHN28_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCQ2299166.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCCTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            9575..9937
                     /gene="rusA"
                     /locus_tag="OHN28_003862"
     CDS             9575..9937
                     /gene="rusA"
                     /locus_tag="OHN28_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCQ2299167.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            9934..10074
                     /locus_tag="OHN28_003863"
     CDS             9934..10074
                     /locus_tag="OHN28_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCQ2299168.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            10160..10543
                     /gene="quuD"
                     /locus_tag="OHN28_003864"
     CDS             10160..10543
                     /gene="quuD"
                     /locus_tag="OHN28_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCQ2299169.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
     gene            complement(10732..11799)
                     /gene="ompC"
                     /locus_tag="OHN28_003865"
     CDS             complement(10732..11799)
                     /gene="ompC"
                     /locus_tag="OHN28_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCQ2299170.1"
                     /translation="MAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSN
                     DADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKF
                     GDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGL
                     VDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQVAYGK
                     SKFNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEAVAQYQ
                     FDFGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINLIDDSK
                     FTKTAGIDTDDIVAVGLVYQF"
BASE COUNT         3275 a         2547 c         2839 g         3138 t
ORIGIN      
        1 gctttctgta tgtctgcggc atgctcccaa taactttccc gaagataaac acccggttca
       61 tctcgtcttt ctcgatcggg tcccacggtg agtagctctt gttatcagag ataaccagca
      121 gcttatcctt catcatttgc aggcgcttta catgggcggt gtcgtcgtac agaaacgcat
      181 agatgccatc accgtcgaaa gatttaaccg tgatatcaac gaacagcaga tcacctggtt
      241 cgatcgttcc tgacatgctg tcaccgcgta cgttaatgat gcggatattt tctgccttcc
      301 taccatcgaa catgtgacga gcatcgtcaa acgagtactc aaccgagcgt agaacttcta
      361 caaactcacg gttgatgact cccggcccgg cactcacttc tatatcaaga acgtcaatct
      421 taaagtattt ggaatggctg acagttgatg gtattggttg cactgtactg tctgacatat
      481 ttccaacgcc agaagataac cattctgcgc gcacacccaa agcgttcgcg atctccacga
      541 ttttagttgt ttggttagct ttccctgttt cgattttctg aatagcagct tggctaaccc
      601 cgaccaaatc cccaagcgcc ttttgtgtaa ggcctcgcgc taatctggct tctttaagtc
      661 tttctgagag tgttgttttc atagtccaaa tgtacaacca aggttttatt ctatcaaacg
      721 aaaatggttg ttgactaaaa acaaccatgg ttttaatctt gattcgaatt aaccacggag
      781 gttgttatga actcagctat caaaacagcg atcaatatcg ttggttcaca aaagaaactg
      841 ggcgctgcct gcgaagtttc acagcaggcc gtctataagt ggcttcacaa caaagcaaag
      901 gtatcccctg aacatgtcgg cagcattgtt acggctactg gtggagtagt gaaggcatac
      961 cagattcgcc cggatcttcc gaagttgttt ccacacaccg aaaagaacgc agcttaaatt
     1021 tccatttcac gctctttaac aataagcaat caacttaaca gtcaattcaa actaaaggag
     1081 tcaattatgc aaccacttac ataccaacag actagcggat ttagcccgac tgcggtgata
     1141 aatcgttctc aaacaaaaca ggtgccaggc cacgaaaaaa tccgtgatgc cgtccgcgcc
     1201 tggtcggctg cagataatca ggatgttgtt gccgcactca ttgtgaatga gtatcgggag
     1261 cagggcggcg gcaccatcga tttccctgat gatgtcagcc gtgcacgcca gaagctgttc
     1321 cgcttcctcg ataacaaatt cgattctgaa aaataccgaa ataacgtgcg tgaactgacc
     1381 ccggcaattc tggcggtact accgctggaa tatcgcggtt acctggttga gcaggatagc
     1441 ttcatgactc ggttggctga aatggaaaag gaactcagtg aggcaaaaca ggctgtcatt
     1501 ctcaacgcac cacgccacca gaaactgaag gaaattagtg aaggtattgt gtcgatgttt
     1561 cgtgtggacc cggatctggc tggtccattg atggcgatgg ttaccaccat gttgggggca
     1621 atatgacagg ctcagaaatg gcgaaagccg gtctgcggga acagagccga ctttcaggtg
     1681 caaatcgtaa cgcactcatt gcggaaggaa ttatggcaaa cactgctgag atattcaatt
     1741 ttccagtgcc ggatgtggca caaaaggagc ggcgcgtggc agatctcgat gatggttata
     1801 cgcgcattgc aaatgagttg ctggaagctg tgatgctggc cggattaaca cagcaccagc
     1861 ttctggtctt tctggctgtc atgcgcaaaa catatggctt taataaaaaa ctggattggg
     1921 tgagcaacga gcaactttcc gaattaaccg ggatattgcc gcacaagtgt tctgctgcaa
     1981 aaagtgttct ggtaaagcgt gggattttta ttcagagcgg gcggaatacc ggcattaata
     2041 atgtggtcag tgaatggtca acattacccg aatcaggtaa gaaaaataaa gtttacctga
     2101 aagaggtaaa tttacctgaa tcaggtaaaa aaagtttacc caaatcaggt aaaggcgttt
     2161 acccgaatca ggtaaacaca aaagacaaac taacaaaaga caatataaaa cctttttcgt
     2221 ccgagaattc tggcgaatcc tctgaccaac cagaaaacga tcttcctgtg gtgaaaccgg
     2281 atgctgcaat tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt
     2341 ggatgtttga catggtgaag accatcgcgc catcagccag aaaaccgaat tttgcagggt
     2401 gggctaacga tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgcg
     2461 tgctgttccg ctgggcatgc caggacaact tctggtccgg taacgtgctg agtccggcta
     2521 aactccgcga caagtggacc cagctcgaaa tcaaccgtaa caagcaacag gctggcgtga
     2581 cagccggaaa accaaaactc gacctgacaa acactgactg gatttacggg gtggatttat
     2641 gaaaaacatc gccgcacaga tgattaactt tgaccgtgag cagatgcgtc ggatcgccaa
     2701 caacatgccg gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa
     2761 cggtgtgttc agccagttac tggcaacttt cccggcgagc ctggctaacc gggaccagaa
     2821 cgaactgaac ggaatccgcc gccagtgggt tctggctttc cgggaaaacg ggatcaccac
     2881 aatggaacag gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac cattcctgcc
     2941 atcacccggg cagtttgttg catggtgccg tgaagaagca tccgttattg ccggactgcc
     3001 aaacgccagc gagctggttg atatggttta cgagtattgc cggaagcgtg gcctgtatcc
     3061 ggatgcagag tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ctagcctgta
     3121 ccagaatatg cgggccaatg cgcttactga tacggaatta cggcgcaagg ctgccgatga
     3181 actggcttgt atgaccgcgc gaattaaccg tggtgaggcg atacctgaac cagtaaaaca
     3241 acttcctgtc atgggcggta gacctctaaa tcgtgttcag gcgctggcga agatcgcaga
     3301 aatcaaagct aagttcgggc tgaaaggagt gatgaaatag agctaaagta ttagcttaaa
     3361 aataaagtat acctagcgaa tataatctag gactagttgt tatgcccgaa atgattgatt
     3421 gcgtgagtaa caaagtgtct cttccattgt tgtgaaatca tttagccata aagtttatgc
     3481 agagatttta ttttattttt tgagttgatt tttttgtaac gatctgtata tttcgcgcct
     3541 cttgtcatac cagttatttt tttcattata ttcataacgt tgattgaact atgaccactg
     3601 tgaataaaaa gttaaaaaaa actgcatctg gcgcgattac atggtcggta attgttaccc
     3661 agatattatc tccagtttct ctttctttga tcccagcaaa cagttttgca tcatctggta
     3721 ataaagatgt tacgcaaact tatgccagcg atgagcatgc aaataaagtg gcctcatttg
     3781 cagcaagtgc aggtcagagc ctagcgaata ataatgcaag tagttttgct gtaaatactt
     3841 tatcaactca ggcgacaaaa gaggtcgtcg actggttgca acaatatggc aatgcgcgca
     3901 tcaagcttaa tgtcgatgat tctttttcct tgaaggattc atcattcgac tttttatatc
     3961 catggctgga tactcaggat tatgtgttat tcagtcagac atcactacat cgtactgatg
     4021 accgaaatca gaccaatatt ggtttgggga ttcgtcattt cactcctgat aacgcaatgt
     4081 tgggtgcgaa tgttttctat gattacgatt taagtcgcag tcactctcgt gcaggttttg
     4141 gggttgagta ctggagggat tatttcaggc ttggtgtaaa tacctatttt ggtctatctg
     4201 actggaagaa cagtcgggat attgataatt atctggaaag acctgcaaat ggctgggatt
     4261 tttctgctga aggatggcta cctgcttatc cgcaattagg ggcatctatt cagtttgaaa
     4321 aatattatgg taaaaatgtc gggttatttg gaagcgataa ccttcaggaa aacccttacg
     4381 cagttactgg gggaatttct tatacaccag ttcctctgat taagttttct gcacagcata
     4441 agcaaggaca gagcaacgtt cacgatacaa ccttcggtgt tgagtttaat tatcgcccgg
     4501 gcgtttccct ggctgaacag cttagcagcg ataatgttgc agttatgcga gaagtccaga
     4561 atcggcgtta tgattttgtt gaacgaaata acaacattgt tctggagtac aagaagaagc
     4621 atgcactgaa aatcagtttg ccagagtctg ttcaggggga gggggaatca atcatccctg
     4681 taacactgac aatcaacaat gctagtggtg gtatcaagtc tgtacagtgg aatgatagtg
     4741 cattcactgc ggctggcggc aagatcagtg gaaatggcac atcatggcag atcactttac
     4801 cggcttataa aagcgaaggt gtaaattcgt ggaatgttgg agccacagta caggataaca
     4861 gaggcaacgt ttccaactat gccgtgatga atattagcgt tactgatagt ggtgtctcga
     4921 cggctgattc gtcttttaca ctggatggag attcgtcgcc gacgatctct gctgatagcc
     4981 aatccactta tccgatagta ttaagcctga aagatagtaa cggtaaggca ttaaccggac
     5041 tggctgatga cattgaaatg tcagtggaat ttacagcaga tagcaatagt gctcgacagc
     5101 gtgaaacggt aactgctccg tcattaggcg cggtagagga aatctctgct ggtgtatatc
     5161 gctctgttct ggctgctggt tcgcaggctg gtacggtacg tgtaactgca aaagttcagg
     5221 gaaaaacatt tactctcagt attaagcaaa cagcagcaac agaacctgat tctgaggtca
     5281 gtgctgtttt aaccgctgca ccagcagaac aggtagttgg ttacaatatc aatctacaac
     5341 tggcagtgaa ggattcacaa ggaaatgcaa tcactggtga taacactctg agcttttatg
     5401 ctcttaacca ggcagaagga gtcgagtttg ggactgtaac ggaaaaagac ggtgtctata
     5461 gcgcgacagt aacttcaaaa cgagctggta agattactat cggtgttaaa tctgatagtc
     5521 atgatttctc aggtgttaag aaagaaatca cctttattga ggaccggcag caggttacat
     5581 tctctcagtt tgaagccagc caaaataatg cattggctga tggaaaacag agaaatatgg
     5641 taactgtaag ccttgcggac cgtttcggca atgttgttcc tggttatgct gtcacattgt
     5701 ctttaccagc agggataact caagttggtg gtgaacatgc tgtttcaact gacgaaaatg
     5761 gtaatgctat ttttgcgtta ataagctcca ctccggggtc ttacgtgatt acagctcatg
     5821 ctggttctca gatgtctact gagttaacag tgacctttgc atcaaatatg accggagctt
     5881 cactgtcact gacgcctgaa agcagtagtt taatttccaa tattcctgct aatggtaagg
     5941 atgcagcggt attagatgtg cagctgacaa atacgaatgc gtcggtcaat gggcaaaaga
     6001 ttcagctcat cgcctcatct gaaggattat ctgtaccaac aaatatcgtg actgattcaa
     6061 cagggcatgt ttctgttccg cttacaacgg tgaaagctgg tgagtacacg gtgactgcac
     6121 gggtgactga cggttcccat agcgtggaat ctggcagcgt taagttaact tttgtgcccg
     6181 atgttgcttc tgcagaactg aatatgagtg tatcaaaaca ggagattgtg gccgatggca
     6241 gtgaaaacgc aacagtaaac attcagttgg ttgatgcgaa taacaatgcg tttacaggcg
     6301 aggtggattt aacgattaca ccatcgacgg gagcatcatt gaccagtagt aatctgcaac
     6361 tggatgctca tggacaggca acaacgcagt tcactgcatc gaaatcaggc cagtacacaa
     6421 ttcaggccga gtatgttctg gatgggaaac gcataaccgc aagccagaat attgatgccg
     6481 tgacggatgt gaagggggct gtgttggaga tcacatcatc tgcatcttcg gctgtagtga
     6541 gcgataccaa caatctggta tttacgttac tgttgaaaag taagtcaggc gaagcattaa
     6601 gtggtcgagc attgaatgtt aagacatctg ggccgtctaa atatggtgcg ttggtggttg
     6661 ataaaaccac agtaacgact gatgagaacg gtcaggctac ggtaagcgta catggtcgaa
     6721 cggttggttc gtataaatta acggcgacct taaacgaact tggcagtgat gttagcgcgg
     6781 tgaaatcatt ctccctgtac gctgatgacg caaatggagt attgactcta acaaaagatc
     6841 ctggttatga gacaaacgat ggatctccag taggtattta tgctcgattt gtcgatcatt
     6901 ttggaaaccc attatctggt acggttgagt tttctgctgg tagtgaagat agtccagact
     6961 ctcaacgggt caaaatggag cctgctacag taacgttaca ctggacaggt aatgcagctt
     7021 cggaattcag cacctatgaa agtggttatc actggattaa ggccaaaata acgaatagca
     7081 aaaatacata tgagaaaaca ataaggactt atgttgtaaa actacctgaa aaggatagtt
     7141 aacaaaattg aatggggagg gcttagccct ccctcttttt acaggttagc gtttgggttt
     7201 tgttgttggg gagctgtttt tataggcgct cccaggtttt tagtaaacca ctcaacgact
     7261 ctgtttataa taattggctg ataccatgtc ttatcgccat gctcggcccc ttctaccagc
     7321 acgtactcag cgttatcacc gttcttcttc aacatcctga atagtttggc gctttgctca
     7381 ggtgaaacca gagtgtcttt gctaccatgc ataataagaa atgggggttt tactcctttc
     7441 atatgtccga ttggactggc gtttagcgct ttttcttttg acgctgtgat gggggctccc
     7501 gcaaaatttc tgaatgcagg gccattgatc attaaggctt cggttacggc aggagagcga
     7561 tgaacctcct gcactgattc agggaacccc tcgccaatat tcaacaagtc agaaatccca
     7621 taaagtgtgg caactgcctg aacatctgag gattgctgaa gaaagtcacc tttatcaaag
     7681 gttttgtcac catttgtagt tcccatcatc tgggcaagcc atccaccggc agagtcaccc
     7741 agaactccga ttctttgagg atcaatccca taattgctgg catgctctct caggtaacgt
     7801 attgcagctt ttccatcctc aactggtgct ggaaatgtat caggaattgt tctgtattct
     7861 acagcggcca caacaaaacc agcttctgcc agagccattc tcatttcaat aaatttgtca
     7921 tgttcagaag acatgaatcc gccgccggga taataaataa tggctggttt taaatcattt
     7981 gttcgcggga caagaactga catgtgaagc tgtctgacag aacgagttcc ttttatctgg
     8041 gaataaacaa catcaccaat gagatcgacc tggtttctgg tcggtgaaac actaatgata
     8101 tctgcacccg gggtgtaacc aggaagatta gtctggacag gtgttgcaca actcccgacc
     8161 gacatggcca ttgacatacc atacaatagt gttgcagatg ataatatctt gttaattttc
     8221 atgttttaca tgccttgact tatcggatga aattctctta ctgaatttaa cggcagttta
     8281 atagccgtac aactcgtggt agccagagaa atatcccagt cgtggttaag gttgtacttg
     8341 acggctatta tttcaacaat gcttaaagtg agagcttaag gttatgcgta tgttttacag
     8401 tccgagtaga ttctgaccaa tagcttcttt gtataattct gtcagaaata aatcttggct
     8461 tgcatgaagt ttgagacctt atcttgtttg attatgaata atcaattcgc cataattgta
     8521 tcaccggagc ctgaacaact ccggtgactt ctgcgctaaa cggggacgtt tatgcgcaca
     8581 cacaatccaa actctcttct cccttcacag atgcagaaat gcacctgcgt ttttttgcat
     8641 ccagcgtctg acctctgtgg aggtgaagcg tgaacctacc acaagatggc atcaaactgc
     8701 atcgcggtaa cttcatcgct atcggccagc agatccagcc ttatctggag gacggaaaat
     8761 gctttcgcat ggtgcttaaa ccgtggcgtg agaaacgcag tctttcccag aatgcactca
     8821 gccatatgtg gtacagcgaa atcagtgaat acctcatcag cagggggaaa tcgttcgcta
     8881 ccgcagcatg ggtaaaagat gctctcaaac acacatacct cggttatgaa accaaggacc
     8941 tggttgatgt cgtaaccggc gaaatcacta ctatccagtc gttacgccat acctccgatc
     9001 ttgataccgg agagatgtat gtcttcctgt gtaaggttga agcctgggcg atgaatattg
     9061 gctgccacct gactattccg cagagctgcg agttccagct gctgcgcgac aagcaggagg
     9121 cgtaatggct acaccgctta ttcgtgtcat gaacggacac atctacaaag taccaaatcg
     9181 tcgtaagcgt aaacctgagc tgaagccatc cgaaatacca acactgctcg ggtataccgc
     9241 cagcctggtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg gctgatttga
     9301 gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc tggcgtatgt aatggcaatc
     9361 ctgaaacgtc agtactggca catatccggc tggctggatt gtgctgtacc ggtatcaaac
     9421 cgccagacct gattgccacc attgcatgtt ctgcctgcca cgacgaaatc gaccgtcgca
     9481 cgcattttgt tgacgctgga tatgcaaaag aatgcgcgct ggaaggtatg gcgagaacac
     9541 aggttatctg gttgaaagag ggggttattg aggcgtgaat acctacagca tcacattacc
     9601 ctggccgccg agcaataatc gctattaccg ccataatcgc ggacgcacgc atatcagcgc
     9661 agaggggcag gcataccgcg ataatgtcgc ccgaatcatt aaaaacgcaa tgctggatat
     9721 cggcctggct attcctgtga aaatcagcat tgagtgccac atgccggatc gccgtcgccg
     9781 tgaccttgat aatctgcaaa aggccgcttt tgacgcactc accaaagcag gtttctggct
     9841 ggatgatgct caggtcgttg attaccgcgt tgtgaagatg cccgttacca aaggtgggaa
     9901 gctggagctg actatcactg aactgggaga tgaatgatgt ttgagtctta tatggcagag
     9961 cgtcttcgcc gccgctgggt gcgcctgcgc ttatatcgtt ttcctggttc tgttttgacc
    10021 gattaccgaa tactgaagaa ttacgccaaa accctgacag gagcaggagt atgaagtcag
    10081 agataacaat caactaatac tgttttgttg atttttgctt gtaattggcg ttctggtctg
    10141 atttttgtgg agtaagttga tgcgtgatat tcagatggtt cttgagcgtt ggggagcgtg
    10201 ggcggctaat aatcatgaag atgtgacctg gtcgtccgtt gccgccggtt ttaagggatt
    10261 aattccttca aaagtaaaat ctcgcccgca atgttgtgac gatgacgcga tgatcatttg
    10321 cgggtgcatg gcccgtctga aaaagaacaa cagcgattta cacgatttat tagtagatta
    10381 ttatgtatgt ggtatgacat tcatgtcact ggcaagtaag cattgctgct cggatggtta
    10441 tatcgggaaa aggttacaga aggctgaggg cataattgaa gggatgttaa tggcattaga
    10501 tatccggtta gatatggata tcgttgctaa taattctaat tgatatgcaa ttgtttacta
    10561 aaagttatta aaaatggggc gtggaaacgc ccccaaaata aagggtaata tataacagaa
    10621 ggtttatata gtaagaagca aggtagtgct tctaaaggaa gtggcttgag ggctccactt
    10681 atatgttgcg gaggcaaagc ctcccgcaac atatcttttt cgtaagtcag attagaactg
    10741 ataaaccaga cctacagcga cgatgtcgtc ggtatcaata ccagctgttt tggtaaactt
    10801 actatcgtca attaagttga ttttgtaatc aacaaaagtg gacatgtttt tattaaagta
    10861 gtaagtagca ccgacatcga catacttgac taagtctcgg tcaccatgaa caccaaggtc
    10921 tttacctttt gactgaaggt aagcaacaga tggtcgcaga ccgaagtcaa actgatattg
    10981 tgctactgct tcaaagtttt gtgctttgtt tgcaatatgg ttattaccaa aaacggtcat
    11041 attctgagtt tcagaatatg tggtagccag atagatattg ttcgcatcat atttcaggcc
    11101 tgcagcccat acttccgcat ttttgccgga ggcattgaat ttgctcttac cataggcgac
    11161 ctgaccgtca gtgcgatctg atttagcata ggttgcaccc acgccgaatc cttcatactc
    11221 ataagtagtg gagaaaccga aaccatcacc attggcttca gttacgtcag tgcggtcatt
    11281 tttaccctga tactgagcag caaagttcag gccatcgacc agaccaaaga agtcgttgtt
    11341 acgataagtt gcaacaccag tagtgcgacc agtcatgaac acatctgttt gggtccaggt
    11401 atcaccaccg aattctggca ggacgtcagt ccacgcaccg atgtcgtatg ctacaccgta
    11461 gttacggccg taatcgattg agccgtaatc accaaatttc aggcctgcaa atgcaagacg
    11521 ggttttgtct ttggaagaac cttgagattc agcacggttg cctttgaatt catattccca
    11581 ctgaccgaaa ccagtcagtt gatcgttgat ttgggtttca cctttgaagc caagacgggc
    11641 ataagtagta tcaccatcat ctgcatcatt agaggagaag tagtgcttgg cattaacttt
    11701 cccgtataga tccagcttgt tactgtcttt attataaatt tcagctgcct gagcagacat
    11761 cgccatcagt actgatgcag ctacagcaga aattgccac
//
LOCUS       DAJOTB010000142        11758 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJOTB010000142 DAJOTB010000000
VERSION     DAJOTB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.142"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..595
                     /locus_tag="OHN28_003866"
     CDS             <1..595
                     /locus_tag="OHN28_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCQ2299171.1"
                     /translation="NYPAEYEIGDIVFTCIGAALFGQISAASNCWNNHVGIIIGHNGE
                     DFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLY
                     HTGFKYESSRQFCSKFVFDTYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(597..1118)
                     /gene="ruvC"
                     /locus_tag="OHN28_003867"
     CDS             complement(597..1118)
                     /gene="ruvC"
                     /locus_tag="OHN28_003867"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCQ2299172.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1153..1893)
                     /locus_tag="OHN28_003868"
     CDS             complement(1153..1893)
                     /locus_tag="OHN28_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2299173.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1922..2374)
                     /gene="nudB"
                     /locus_tag="OHN28_003869"
     CDS             complement(1922..2374)
                     /gene="nudB"
                     /locus_tag="OHN28_003869"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCQ2299174.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2367..4139)
                     /gene="aspS"
                     /locus_tag="OHN28_003870"
     CDS             complement(2367..4139)
                     /gene="aspS"
                     /locus_tag="OHN28_003870"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCQ2299175.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4449..5015
                     /locus_tag="OHN28_003871"
     CDS             4449..5015
                     /locus_tag="OHN28_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2299176.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5012..5830
                     /locus_tag="OHN28_003872"
     CDS             5012..5830
                     /locus_tag="OHN28_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCQ2299177.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRYAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5883..6278
                     /locus_tag="OHN28_003873"
     CDS             5883..6278
                     /locus_tag="OHN28_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCQ2299178.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6319..7062
                     /gene="cmoA"
                     /locus_tag="OHN28_003874"
     CDS             6319..7062
                     /gene="cmoA"
                     /locus_tag="OHN28_003874"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCQ2299179.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7059..8030
                     /gene="cmoB"
                     /locus_tag="OHN28_003875"
     CDS             7059..8030
                     /gene="cmoB"
                     /locus_tag="OHN28_003875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCQ2299180.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSHLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIAHKP"
     gene            complement(8066..10495)
                     /gene="torZ"
                     /locus_tag="OHN28_003876"
     CDS             complement(8066..10495)
                     /gene="torZ"
                     /locus_tag="OHN28_003876"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCQ2299181.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAIQA"
     gene            complement(10520..11620)
                     /locus_tag="OHN28_003877"
     CDS             complement(10520..11620)
                     /locus_tag="OHN28_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCQ2299182.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDSALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2694 a         3108 c         2869 g         3087 t
ORIGIN      
        1 taattatcct gcggaatatg aaattggtga tatcgtcttt acatgtatag gtgctgcctt
       61 atttggtcaa atatcagctg catcaaattg ctggaataat cacgtcggga tcattatcgg
      121 tcataacggt gaagactttc tggttgcaga aagccgtgtt cccctctcaa ccatcactac
      181 gctatcccgt tttattaaac gctctgctaa tcaacgctat gctataaagc gattagacgc
      241 cggactaaca gaacaacaaa aacaacgaat tgttgaacag gttccttccc ggctacgcaa
      301 actttaccac accggtttta aatacgaatc ttcgcgacag ttctgttcaa aatttgtttt
      361 tgatacttat aaagaggcgc tatgtattcc ggtgggtgaa atagagacgt ttggagaatt
      421 attaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttct taggttctat
      481 tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg gtttggtgtt
      541 gattcacgcg gtgggagttg aaacgcctca gcctgaactg accgaggcgg tataacttaa
      601 cgcagtcgcc ctctcgccag gttcagtcgc gattcactca tttgcatcgc attctgacta
      661 acgtggcagt gggtgatggc aatcgccagc gcatcggcgg catccgcctg tggattagcg
      721 ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc actaccaata
      781 cctaccactg tttgctttac ctgacgtgcc gcatattcaa ataccggcaa ttcctgattc
      841 accgccgcca caatcgccac gccgcgcgcc tgccccagtt tcagggctga gtcagcgttc
      901 ttcgccataa agacttgttc aatggcgaaa taatcaggct ggaattgggt gatgatttcc
      961 gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt ggtgcgtatg
     1021 catccgctac ccaggtagga cagttgcctg cctacctggc ggatgacgcc atagccggtc
     1081 acgcgcgaac ccgggtcaat accgagaata atagccatca cgcgtctccg ttttgctgtt
     1141 tagcaggcct catcagagag tcgctgcaac ctcatcagag atttcaccgt tatggtaaac
     1201 ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt tcggtgcggt
     1261 ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc tgtctgcttt
     1321 cagacctgcc gcttccagag cgtcgcgtac tttgcccatt tcttcccatg cagtgtagac
     1381 atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca ccggcttcca gggctgcttc
     1441 catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc tgaacagata
     1501 agctacggaa ccatcagtac cgaggttacc gccacatttg ctgaatgcat gacgcacttc
     1561 agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg tgccgccagg
     1621 accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc ccacacctcg
     1681 tgcaattgcg cggttcagag tgtcacgggt catgttgtta gacagtgctt tatcaattgc
     1741 tgcacgcaga cgcggattag cgtccggatc accaccgccc agcttagccg cggttaccag
     1801 ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag ctttacgatg
     1861 tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata gccctgcctg
     1921 ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag gacttagtga
     1981 gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg aaaacgatct
     2041 ggcgctcgtg cgggagcgca agacagaacc atgattccgt attacgcgtc acgcccggcg
     2101 catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga cagtcaatta
     2161 aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg cgcatggcag
     2221 cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag aaatcgggat
     2281 cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcgtagatg accactaaga
     2341 tcgaaacggg acgcttataa gccatatcag ttattctcag ccttcttcac aacctgaatg
     2401 ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat cagacacgcc
     2461 gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc ggtcagcagc
     2521 atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc gtatttcagg
     2581 gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc cagaatacca
     2641 aacaccgtct gctgcatatc accattatgg atacgcacag aaccaccgcc cacttcgtaa
     2701 ccattgatga ccatatcgta agcgttagcc accgcatttt ccggtgcagc tttcagttct
     2761 gctgccgtca tgtctttcgg tgaggtgaac ggatggtgca ttgctgtcag gccgccttca
     2821 ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt gctttcgtcg
     2881 gtcagaccaa ggtctttacc cactttcagg cgcagtgagc ccatcgcgtc ggcaacaatt
     2941 ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcgccagt acgctccagg
     3001 atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat accttccaga
     3061 cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta gattttaacg
     3121 aagttaccgt attcgtcgat ctgcttacgg gtcaacgatg cgccgcccgg aacacgcaga
     3181 gcggcaacac ggcctttcgg atcgttcgcc ggacctgcaa atactgcaaa ctcaacagat
     3241 ttcagcagat cggcaacgtc ggtcagttcc atcgggttac gtagatctgg tttatcagaa
     3301 ccataacggc gttctgcttc tgcaaaggtc attaccggga aatcgcccag atccacgccc
     3361 ttcacttcca gccacagatg acgcaccagc gcttccatca cttcacgcac ttgcggcgcg
     3421 gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacggtc agcacgcagg
     3481 tcttcgtcac ggaagcattt aacgatctga tagtagcggt caaagccgga catcatcagc
     3541 agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt gtgcacacga
     3601 gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat cggagtttcg
     3661 atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat tttagcgcgg
     3721 gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta tttcagacgc
     3781 gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcacg gttgatgata
     3841 gtcagcgagg acgccagtac ttcgatttcg ccagtcgcca tatcgcggtt aatatttttt
     3901 tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg cagttcagag
     3961 gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat accttcgcgg
     4021 tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac ccaaccacac
     4081 agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc tgtacgcatg
     4141 agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga ccaagtcgca
     4201 gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg aaattctgcc
     4261 gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat cgggcctgaa
     4321 atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa attaacaaaa
     4381 tttgtcgttc cgccatcggc tcatcgtatt aaggtgagag gcacgatttt gttttatcag
     4441 gagtcatcat gcttgaactt aatgctaaaa ccaccgcgct ggtggtgatt gatttacaag
     4501 aaggcatctt gccttttgcc ggtggtccac atactgccga tgaggtggtt aatcgcgccg
     4561 ggaagctggc ggcgaaattt cgcgcgagcg gtcagcccgt gtttctggtg cgtgttggct
     4621 ggtctgccga ttatgctgaa gcattaaaac agccggttga tgccccttcc cccgctaaag
     4681 tgttgcccga aaattggtgg caacatcctg tagcattagg tgcaaccgac agcgatatcg
     4741 aaatcatcaa acgtcaatgg ggtgcgtttt acggtacgga tctggaattg cagttgcgcc
     4801 gccggggtat cgatacaata gtgttatgtg ggatctcgac caatatcggt gttgaatcca
     4861 ccgcccgcaa tgcctgggaa ctcggtttta atctggtgat tgccgaagat gcctgtagcg
     4921 ccgctagcgc cgagcagcac aataacagca ttaatcatat ctacccgcgc atcgcccgtg
     4981 tgcgtagcgt tgaagagatc ctccacgcgt tatgatttac atcggtttgc cacaatggtc
     5041 gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc gccactttaa
     5101 ctgtgtggag ggtaacacca cgctttatgc cctgccgaaa cccgaggttg tcctgcgctg
     5161 gcgtgagcag accacagatg acttccgctt ctgttttaag tttccggcga ctatttcgca
     5221 tcaggcagca ttacggcatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
     5281 gttggctccg cgcattggac aatactggct gcaactgcct gccacattcg gcccacggga
     5341 gctgcctgcg ctttggcatt ttctcgattc tcttcccggc gaatttaatt atggtgtgga
     5401 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
     5461 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg cagcacgtcc
     5521 acacagtgaa gctattcgct acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     5581 actgacggcg aaaaatccac tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
     5641 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
     5701 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
     5761 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     5821 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     5881 gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     5941 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg cgacggcggt tttagcgaac
     6001 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgt
     6061 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     6121 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg ttccgctggc
     6181 gacgttctgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     6241 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     6301 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     6361 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     6421 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     6481 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac actctcggtg
     6541 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgataactc cccggcgatg
     6601 attgaacgct gccgtcgtca tattgacgcc tataaagctc ctacgccagt agacgttatt
     6661 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     6721 ctgcaattcc tggaaccttc cgagcgccag gcgttactgg ataaaattta tcaaggactg
     6781 aacccgggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     6841 gaactgctgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     6901 atcagccaga aacgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     6961 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     7021 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     7081 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc acgcagattg
     7141 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac gcggtggaat
     7201 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     7261 aagagccact gagcgccggg caaattaagc gcattgaaac gctgatgcgc aacctgatgc
     7321 cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     7381 actggaaatg ggatcgcgtt ctgccccatc tttctcattt aaccgggcgc accattcttg
     7441 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgctggc gcgcacctcg
     7501 cggtggggat cgaccctaca caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     7561 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacaactt ccggcactga
     7621 aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca ccgctggagc
     7681 atctctggca gttaaaagac caactggtga atgaaggcga actggtgctg gaaacgctgg
     7741 ttattgatgg cgatgagaac acggtgctgg tgccgggcga tcgttacgca caaatgcgta
     7801 atgtctattt cattccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     7861 ttgatattcg cattgcggat gtgagcgtta ccaccacaga agagcagcga cgcaccgaat
     7921 ggatggtcac cgagtctctg gccgattttc tcgacccgca tgatccgggt aaaacggtgg
     7981 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgca caagccgtaa aaatttagcg
     8041 cccgtttccg ggcgcttttt acgtcttatg cctgaatagc tggctgatca aacgccgtta
     8101 acttcagcgc attgcccgta tatttttcga tatacaccag cgcagagtta ccggcacagg
     8161 cattcgccag ctgcgagctg gggatatccg ccgttaacac gttcgcacta ccgtttttac
     8221 acaagccatt ttccagatct ggccatgcac cttcatgcac gcataccacg ccttttttga
     8281 tcccgtcagt caccaccgcg cctgtcagaa tctgaccacg tttgttccag acacgcacca
     8341 gatcgccatt cgcaatacca aagcgagcag catcttcggt gtgaatagtg attggttcac
     8401 gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg tgtaaacggt
     8461 gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc ttccactcat
     8521 caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt tccagcgttc
     8581 tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc ggatcggcgc
     8641 ggaaatcacc ataacgaatg tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt
     8701 tattttgctg ccagaaggca ttaaacatcg gcatcgtcac gcgttgcgca cgggcacctt
     8761 tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct tcggtataga
     8821 tctcttttcc gccaggtttg agtaattcag caagatcggc aaacacgtca aaatcgttgc
     8881 gcgcttcaaa ttgcggagcg acagcctgct tcatcggcac aatatgctgg ttgctgtaat
     8941 caccggtcat cgtcaggtca ttgcgctcaa acgatgtggt gatcggtaat acgatatctg
     9001 cgtgtttggc tgctgcggtc cagtagcatt cagaaacgac gatcatctcc ggtttctgcc
     9061 atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca cccgcccacc
     9121 agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat ttgccgccag
     9181 gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg ccatcatcag
     9241 cggcctcgct ggcctgtccg gcaatcgccg cagacatttc tggtagcacg ccgccaacac
     9301 gcgtcgggtt gccaccgttg gagtaatgat aagagaaacc aaacccgccg cccggtgtac
     9361 caatttgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt tcaccgtatt
     9421 gctggcgctg aattccccag cctgccatca gcattgtgcg atttgccgcc atcagttctg
     9481 caagtttcac aatctgcgct tccggcacgc cagtaatttc cgccgcccag gccgcactct
     9541 tcggcgtatt atcgttcttg cctgtcagat actcttcaaa ctgcggatac ccggtagtgt
     9601 atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta
     9661 acatcagtgc cacgtcggtg cccatattcg gtgcgatcca ggtggcatta tcgccaaaga
     9721 attcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca gattttttca
     9781 gctgatggaa gtattccagc ccttgttcat cggtactgct ccaggcaatt tttaaggtat
     9841 tcagcgggtt catgccccac aacaccacca cctgactgtt ttccagaatc agcggccagg
     9901 aagtctgctg ttcatagacc tccacagaac cgaccacatg cggcatgatc acctgtgccg
     9961 ctccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc atataacgtt
    10021 gcagtaaggt ttgcgcttta tgcaacacgc cagaagaacg ccagccgtaa gatccggcaa
    10081 aaatggccga tggtccgtta gctttacgaa tatgatcgtg ttgctcatga atcagtttta
    10141 atgcttgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc gccggttgca
    10201 gtggattatc gagatagctt tttctcacca tcggatgctg aatacgcgcc gtggtgtgta
    10261 cctgatccgc cgccgtagac tgtaaggagt tcggtatggt cttcgccagc gcgcctgtcg
    10321 aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt cccgcggtga
    10381 gaattttgcc gcccttctct tctgcccatg caggtaacgg tgcagccgat gtcaccacca
    10441 gcgccccagc ggcaataccg ctatgtttaa taaattcgcg tcttgttaat gtcataactt
    10501 cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg tactggaagt
    10561 accgcgtcaa aatgtccagt tcgttttcgc tcatgctggt tcgcgcaccc attcctttgg
    10621 caatggacgg ccatgcattg acggtgtaat ggtcggcggc aataggggca tggcagccag
    10681 cgcaataggt atcgtcaagt ttttcagcgt attgccatag tggtttacgg tccgctaatg
    10741 cggaatcggt tagcgcaccc tgtaaagacg cctgacgcca ttgattgccg tattcgtcag
    10801 cctgccattc cccgtttacc gttagcgcct tgataccttc ttcacttaat gtggcgagcg
    10861 ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt tgataaccct
    10921 gcaaccgaac aattggctgt ttaccactga catcaacgac ggtgagatcg gtaccaggag
    10981 tcacggtagc caggtcgcct atgcgagaag ttttgaaagg ataaatatgt gcgccattag
    11041 taactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc atttttattt
    11101 ctggcgggaa atgggcaatg cctttatggc aatcgataca agtttcgccg tccttttgcg
    11161 ctttattatg cattttctgc gcagattcac tttgcgagga aatatccatg gcatcaaaag
    11221 aatggcaact acggcaggtt gcagagtcag ttgcttttaa ttctttccat actgtttcgg
    11281 ccatttcctg gcgatgagct tcgaacttat cgtcactgtc tattttgccg ctaacaaatt
    11341 catgataaat atctttagat gcttttaatt tagcaaataa ataatccatc cctgactttg
    11401 gaatatggca atcggcacat tccgcgcgta tacctttttg gttcgaaaag tggacagttc
    11461 cctgatattc ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat gcagtatccg
    11521 acgttttatg taaggctttt tgcgccagca ataacccgcc gccaccaacc acaaccgcta
    11581 tcagcaaaaa caataaccca atgcgttttt tccctcgcat agattcctct aattaaattc
    11641 caggacaccg ataccattgt ataaataaat atacaacgaa ttgactactt gcttaattcg
    11701 gcacaacact gcctgaaata atcgataaag aatatgattt attacaatgt aatcatta
//
LOCUS       DAJOTB010000143        11619 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJOTB010000143 DAJOTB010000000
VERSION     DAJOTB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.143"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..804
                     /locus_tag="OHN28_003878"
     CDS             <1..804
                     /locus_tag="OHN28_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299183.1"
                     /translation="ALKIKEPRNKESLFFMSAMELRDLLIALESRLHQKHKLDAAARL
                     QYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDDQGENLTFVLTLHDGSK
                     CELVVNELQIEMLARAIIHAINNAEMRGLALRITSLLDFLPLYDVDCQDDGNLEYDTY
                     SQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEVEAITRRMLDFSPR
                     LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(854..1207)
                     /locus_tag="OHN28_003879"
     CDS             complement(854..1207)
                     /locus_tag="OHN28_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCQ2299184.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1345..2991)
                     /gene="groL"
                     /locus_tag="OHN28_003880"
     CDS             complement(1345..2991)
                     /gene="groL"
                     /locus_tag="OHN28_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2299185.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3035..3328)
                     /locus_tag="OHN28_003881"
     CDS             complement(3035..3328)
                     /locus_tag="OHN28_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2299186.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3604..4860
                     /gene="yjeH"
                     /locus_tag="OHN28_003882"
     CDS             3604..4860
                     /gene="yjeH"
                     /locus_tag="OHN28_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCQ2299187.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4876..5352)
                     /gene="fxsA"
                     /locus_tag="OHN28_003883"
     CDS             complement(4876..5352)
                     /gene="fxsA"
                     /locus_tag="OHN28_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCQ2299188.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5689..7125
                     /gene="aspA"
                     /locus_tag="OHN28_003884"
     CDS             5689..7125
                     /gene="aspA"
                     /locus_tag="OHN28_003884"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCQ2299189.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7243..8544
                     /gene="dcuA"
                     /locus_tag="OHN28_003885"
     CDS             7243..8544
                     /gene="dcuA"
                     /locus_tag="OHN28_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCQ2299190.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8660..8998
                     /gene="cutA"
                     /locus_tag="OHN28_003886"
     CDS             8660..8998
                     /gene="cutA"
                     /locus_tag="OHN28_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCQ2299191.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8974..10671
                     /gene="dsbD"
                     /locus_tag="OHN28_003887"
     CDS             8974..10671
                     /gene="dsbD"
                     /locus_tag="OHN28_003887"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCQ2299192.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10708..11283
                     /locus_tag="OHN28_003888"
     CDS             10708..11283
                     /locus_tag="OHN28_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2299193.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11390..11465
                     /locus_tag="OHN28_003889"
     tRNA            11390..11465
                     /locus_tag="OHN28_003889"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11423..11425,aa:Phe,seq:gaa)
BASE COUNT         2776 a         3023 c         2859 g         2961 t
ORIGIN      
        1 gcgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc tgccatggaa
       61 ttgcgcgacc ttctgattgc gctggaaagt cgtctgcatc aaaagcataa actggatgct
      121 gctgcacgcc ttcagtatga acaagctcgc gataaagtca ttaagaaaat ggcagaaaat
      181 atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg ggttaacacg
      241 ctggaactta ccgatgatca gggcgaaaat cttaccttcg tattaacact acatgacggt
      301 agcaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg agcaatcatt
      361 catgcaatta ataatgcgga aatgcgtgga ctggcattac gtattacctc actgttagat
      421 ttcctgccgt tatatgatgt cgactgccag gatgatggta atctggaata tgacacctat
      481 tcccaaccgg aatggaaaca taacttgttc aaccattatc tggcggtgct ttaccgcttt
      541 aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg
      601 ccgggtaaag aagtggaggc gatcactcgc cgaatgcttg atttcagccc ccgactgaaa
      661 aaactcgcgg gtgtgccatg tcaggtctat gttcgcaccg tagcggcaaa taacgcccaa
      721 ccgctaaccc aggatcaatg cttacgcgcg ctgcatcact tacgtgttca atccaccagc
      781 aaaactgcac cacaggcgaa ataatcttat ctgttttact acgaggctgg aagtatccag
      841 cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg ctatccgtcg
      901 ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc acgttaccgc
      961 ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa ccgccttctt
     1021 ctccgtgttg cccggaaagc cagttgcttt gcttacctgt tgcagtacca atcagctggc
     1081 actctgcacc tggttgctcg tccacaatgc gtacactctg accggcagca ctcaattcgt
     1141 tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct aagtatttta
     1201 cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc cgtgtctgaa
     1261 tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc gggggtttgt
     1321 ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca tgccgcccat
     1381 accgccagca gcgcctaagt cagctgcatc gtttttcggc aggtcggtaa ccatgcactc
     1441 ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac gggttacttt
     1501 ggttggatcc aggataccca tatcgatcat gttgccgtat tcttcagttg ctgcgttgta
     1561 accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt cttcgccgca
     1621 gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt tgatacccac
     1681 gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc ggatcagcgc
     1741 aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt gcagggcgtc
     1801 ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccaa ctttgataac
     1861 tgcaacgccg cctgccagtt tcgctacgcg ctcctgcagt ttttcacggt cgtagtcaga
     1921 agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg cagcttcttc
     1981 acccacgcca tcgatgatgg tggtggtgtc tttgttgatc acaacacgtt tagcctgacc
     2041 caggtcttcc agggttgctt tttccagctc cataccgatc tcttcagaga ttacggtacc
     2101 gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga agcccggagc
     2161 tttaaccgca gcaactttca cgatgccacg catggtgtta acaaccagag ttgccagtgc
     2221 ttcgccttca acatcttcag cgatgatcag cagcggttta cctgctttgg caacagcttc
     2281 cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca ggatgaacgg
     2341 gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag acaagtagcc
     2401 acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg taccgtcttc
     2461 aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca gtttacctac
     2521 ggtttcgtcg gagttagcag agatggtacc aacctgagca atcgctttag agtcagagca
     2581 cggtacggac agcgctttca gttcttcaac tgcagcggta acagctttgt cgataccacg
     2641 tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag tgatgatagc
     2701 ctgagccagt acggttgcag tggtggtacc gtcgcctgca gcgtcattcg ctttagaggc
     2761 aacttctttc accatctgcg cacccatatt ttcgaacttg tcttccagtt cgatttcacg
     2821 agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat ccagaactac
     2881 gttacggcct ttaggaccga gggtaacttt cactgcatct gccagtacgt ttacgccgcg
     2941 cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca ttatctttat
     3001 tccttaaatt cgtatgttca gtgtcgtgcg cggattacgc ttcaacaatt gccagaatgt
     3061 cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc acaccgtagc
     3121 catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact tcgccatttt
     3181 caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta gccgctgcag
     3241 agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc ttgacgatca
     3301 cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag tccgtatctg
     3361 ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa atggggatga
     3421 ggcttcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa aatcgggtga
     3481 aaaccctgat tcacctcaca tttcatcgct atttcttcat caccgtcata agcaaatctg
     3541 attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat cggacatcaa
     3601 cccatgagtg gactcaaaca agaactgggg ctggcccagg gcatcggcct gctatcgacg
     3661 tcattattag gcactggcgt gtttgccgtt cctgcgttag ctgcgctagt agcaggcaat
     3721 aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc gattgtgttt
     3781 gcgattctgg gtcgccacta tcccagcgca ggcggcgtcg cgcacttcgt cggtatggcg
     3841 tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat tcccgtgggt
     3901 ttgcctgccg cgctacaaat tgctgccgga ttcggtcagg caatgtttgg ctggcatagc
     3961 tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat cggtactcgc
     4021 ggtgccagtt ccagtgctaa tctacaaaca gttattgccg gacttatcgt cgcactgatt
     4081 gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc tgcgccagga
     4141 aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg ttttgtcggt
     4201 ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga ttttcctcgt
     4261 gctttgatga ttggcctgct gctggcagga ttagtctatt ggggctgtac ggtagtcgtc
     4321 ttacacttcg acgcctatgg tgaacaaatg gcggcggcag catcgcttcc caaaattgta
     4381 gtgcagttgt tcggtgtagg agcgttatgg attgcctgcg taattggcta tctggcctgc
     4441 tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc gcaggcgcaa
     4501 cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa taatgccctc
     4561 aatgcggtgc tcggctgctg cgtggtaagc acgttggtga ttcatgcttt agagatcaat
     4621 ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct gttatgcatg
     4681 ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt tggcgggcta
     4741 ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctacgcgct gatcatgctg
     4801 gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat aaccacataa
     4861 tccggcgttt cgacattaat cctggcgatc gtctttatga tcaaggcggt cgcggtcatc
     4921 atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg cgctaaaacc
     4981 gccgcctggc atgcgagaaa agcgcaaatg cggcatcaac ttcactgtca gatgcttttg
     5041 caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg gtaataaaag
     5101 cagcaaaccg gcaatgatca gcgaaacact tttaatcatc tccgccgctg ggttttcacc
     5161 cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac gtaccagtga
     5221 cataccgata actgacgtga atataaccag cacgagggtc agcaataccc ccaatacatg
     5281 agcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg caataaaagg
     5341 taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta cctgaatggg
     5401 ttgcgaatcg cgtttagctt atattgtggt cattagcaaa atttcaagat gtttgcgcaa
     5461 ctatttttgg tagtaatccc aaagcggtga tccatttcac aaattaataa ttaaggggta
     5521 aaaaccgaca cttaaagtga tccaggttac ggtagaaatc ctcaagcagc atatgatctc
     5581 gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa ccacatatat
     5641 tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat gtcaaacaac
     5701 attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc ctactatggt
     5761 gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat cagtgatatt
     5821 cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc aaacaaagag
     5881 ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga tgaagtcctg
     5941 aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg cgcaggtact
     6001 tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact gatgggtcac
     6061 cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg tcagtctact
     6121 aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat taaactggta
     6181 gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt ccaggacatc
     6241 ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg tcaggaattc
     6301 cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac cgctgaactg
     6361 ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac gccgaaagag
     6421 tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg cgtaccggct
     6481 gaagacctga tcgaagcgac ctccgactgc ggcgcttatg ttatggttca cggcgcgctg
     6541 aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct ctcttctggt
     6601 ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcaggctc ttccatcatg
     6661 ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt caaagtcatc
     6721 ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt gaacgttatg
     6781 gagccagtca ttggccaggc catgttcgaa tccgttcaca ttctgaccaa cgcttgctac
     6841 aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg cgaaggttac
     6901 gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca ccacaacggt
     6961 gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt cgttctggaa
     7021 cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa tctgatgcac
     7081 ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta cagggtagta
     7141 caaataaaga aggcacgtca gatggcgtgc cttttttctt gtgagcagta acttaaaaat
     7201 aacaatttaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt tgtagaactc
     7261 atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg tattggtttt
     7321 gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg taacatcccg
     7381 ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat gcaggttgct
     7441 ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa cccgaaatac
     7501 atcacgatcc tcgcaccgat cgtgacctat ttcctgacta tctttgctgg tactggcaac
     7561 atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg cgttaaacct
     7621 tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc atcgccaatc
     7681 tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag ctacctccat
     7741 ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc cttcctggtc
     7801 actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg tctggaagag
     7861 ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc aaaaacgtcc
     7921 gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat caacagccca
     7981 agcatgggtc tggttgaaaa accactgatg aacaccacca acgcaatcct gatcatcatg
     8041 ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa cattctcaac
     8101 tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt tgcgtggctg
     8161 ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg tgaagtgatt
     8221 cagggtcacc cgtggctgct ggccgtcatc ttcttctttg cttctgctct gctgtactct
     8281 caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt ttctccgctg
     8341 accgctgttg cttcttttgc tgcggtgtct ggtctgttca ttctgccgac ctacccgacg
     8401 ctggttgctg cggtacagat ggatgacacg ggtactaccc gtatcggtaa attcgtcttc
     8461 aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg cttcggcttc
     8521 gtgctgggta gcttcatgct gtaatgaccc atcgcggggc gttcacgccc cgctttcttt
     8581 cccgccgact aacatccttt ccccgtccgt tgtatagtga cctctctctt gcagttccat
     8641 ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccacgt ctgtcgtggt
     8701 gctatgtacg gcaccggatg aagcgacagc ccaggattta gccgccaaag tgctggcgga
     8761 aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt actgggaagg
     8821 taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat ctcaccagca
     8881 ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac ttctggtttt
     8941 acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt tacgctgatc
     9001 ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg acgttcacaa
     9061 tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca tgaccttaat
     9121 ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg cattacgccg
     9181 gaacacgcga aaattgctga cgtgcagctg ccgcaaggcg tctggcatga agatgagttt
     9241 tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat caaccaggca
     9301 agtgcgggag caacgttaac tgtcacctac cagggctgtg ctgatgccgg tttctgttat
     9361 ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacga agcgtcacag
     9421 cctgtgtctg ttccgcagca agagcagccc accgcgcaat tgcccttttc cgcgctctgg
     9481 gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat gtacccactg
     9541 atttctggca tcgtgctggg cggtaaacag cggctttcca ctgccagagc attgttgctg
     9601 acctttattt atgtgcaggg gatggcgctg acttacacgg cgctgggtct ggtggttgcc
     9661 gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat tggcctcgcc
     9721 atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctgca actcccctct
     9781 tcgctgcaaa cacgtctcac gctgatgagc aatcgccaac agggcggctc acctggcggt
     9841 gtgtttatta tgggggcgat tgccggactg atctgttcac catgcaccac tgcaccgctt
     9901 agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg cggcacgctt
     9961 tatctttatg cgctgggcat gggcctgccg ctgatgctaa ttaccgtctt tggtaaccgc
    10021 ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg ttttgtgatc
    10081 ctcgcactgc cggtcttcct gctggagcga gtgattggtg atatatgggg attacgcttg
    10141 tggtcggcgc ttggtgtcgc attctttggc tgggccttta tcaccagcct acaggccaaa
    10201 cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt tagcgtacgc
    10261 ccacttcagg attgggcatt tggtgcaaca cataccgcgc aaactcagac gcatctcaac
    10321 tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc caaaggcaaa
    10381 ccagtgatgt tagatctcta tgccgactgg tgcgtcgcct gtaaagagtt tgagaaatac
    10441 accttcagcg actcgcaggt gcaaaaagcg ttagcagata cggtcttact tcaggccaac
    10501 gtcactgcca acgacgcaca agatgtggcg ctgttaaagc atcttaatgt ccttggccta
    10561 ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg cgtcacgggc
    10621 tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg aacgacactt
    10681 gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg agaagccctg
    10741 aaattattag aattacaagg gattgccaac accacgctgg agatggttgc tgaacgtgtg
    10801 gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat cctctacgat
    10861 gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat gctggacgaa
    10921 acgcaaaccg ccgaacaaaa gttgctggca cgttatcagg cgttatcgga gtgcgttaaa
    10981 aacaaccgct atccgggctg tctgtttatc gctgcctgta cgttttatcc cgatcctggc
    11041 caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt cacccacgaa
    11101 ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat ggaactggtg
    11161 ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt cgacaccgca
    11221 catcggctgg cggaagatat cctgcgcttc gcccgctgcc gtcagggtgg tgcactgacc
    11281 tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa tcgcttttac
    11341 tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg cccggatagc
    11401 tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc cgagtccggg
    11461 caccaaattc acttccagat tcatctcccg aactccacga aactcaagta taatcacatc
    11521 gttacaagca atctgaacat cctgaactcc accgaactct actgaactct ctctcaatca
    11581 agcgttcatt gggggcaccg gtgggggctc attgtgttc
//
LOCUS       DAJOTB010000144        11296 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJOTB010000144 DAJOTB010000000
VERSION     DAJOTB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.144"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            202..306
                     /locus_tag="OHN28_003890"
     CDS             202..306
                     /locus_tag="OHN28_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCQ2299194.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            616..2814
                     /gene="speF"
                     /locus_tag="OHN28_003891"
     CDS             616..2814
                     /gene="speF"
                     /locus_tag="OHN28_003891"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCQ2299195.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2811..4130
                     /gene="potE"
                     /locus_tag="OHN28_003892"
     CDS             2811..4130
                     /gene="potE"
                     /locus_tag="OHN28_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCQ2299196.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4187..5827)
                     /gene="pgm"
                     /locus_tag="OHN28_003893"
     CDS             complement(4187..5827)
                     /gene="pgm"
                     /locus_tag="OHN28_003893"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCQ2299197.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5853..6398)
                     /gene="seqA"
                     /locus_tag="OHN28_003894"
     CDS             complement(5853..6398)
                     /gene="seqA"
                     /locus_tag="OHN28_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCQ2299198.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6583..7347
                     /gene="ybfF"
                     /locus_tag="OHN28_003895"
     CDS             6583..7347
                     /gene="ybfF"
                     /locus_tag="OHN28_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2299199.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7487..7780
                     /gene="ybfE"
                     /locus_tag="OHN28_003896"
     CDS             7487..7780
                     /gene="ybfE"
                     /locus_tag="OHN28_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCQ2299200.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7920..8450
                     /gene="fldA"
                     /locus_tag="OHN28_003897"
     CDS             7920..8450
                     /gene="fldA"
                     /locus_tag="OHN28_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCQ2299201.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8660..8746
                     /gene="uof"
                     /locus_tag="OHN28_003898"
     CDS             8660..8746
                     /gene="uof"
                     /locus_tag="OHN28_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCQ2299202.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8739..9185
                     /gene="fur"
                     /locus_tag="OHN28_003899"
     CDS             8739..9185
                     /gene="fur"
                     /locus_tag="OHN28_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCQ2299203.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9269..9595)
                     /locus_tag="OHN28_003900"
     CDS             complement(9269..9595)
                     /locus_tag="OHN28_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299204.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9645..11051)
                     /gene="chiP"
                     /locus_tag="OHN28_003901"
     CDS             complement(9645..11051)
                     /gene="chiP"
                     /locus_tag="OHN28_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCQ2299205.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2772 a         2798 c         2784 g         2942 t
ORIGIN      
        1 aggtttaatt tcttaatgaa aatacaaccc acaaaattgc aacatttaaa caacaaaaca
       61 gcatctaata tggcgctatt catcattaat gatttattat ttgggatatg atcgtttttt
      121 gttgatcttc ttcacagatt atagccattt catggataga ataaccctac cttcaactga
      181 cacagaaaaa ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg
      241 acaacccata tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc
      301 cgctagtctt ctgactaaag ggcaccccag cgtacaggtc tccctgactt taagcattac
      361 aggttaatac ctgtatccct cggtgctcat atactgctaa cccttttaaa ctctaaataa
      421 ttcgagtcgc agcacatgca acttgaagta tgacgagtat agccagttac cgggctggtc
      481 tgggttattg catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggggttttgc
      541 ttttttccgg cgtgatcaat ttctcctttg agaaattgag gacctgctat tacctgaaat
      601 aaagagatga acaaaatgtc agaattaaaa attgccgtta gccgttcttg cccggattgt
      661 ttttccactc atcgtgaatg cgtgaatatt gacaaaagta attatattga cgttgccgcc
      721 attattttat cagtcaatga tgttgaacat ggaaaactcg atgaaataga cgctactggc
      781 tatggcattc ctgtttttat tgcaacggaa aatgaagaac gtgtaccagc agaatatctg
      841 ccacgtattt ctggtgtctt cgagcattgc gaatcacgga aagagtttta tggacgtcag
      901 ttagaaaccg ctgccagcca ttatgaaacg caactgcgcc cgcccttctt ccgcgcactg
      961 gtcgattatg tcaatcaggg caatagcgct tttgactgcc cgggtcatca gggaggtgag
     1021 tttttccgtc gccatccggc aggcaatcag ttcgtggaat attttggcga aatgctcttc
     1081 cgctccgact tgtgtaatgc cgacgttgca atgggcgatc tactgattca tgaaggtgcg
     1141 ccatgcattg cacagcaaca tgcggctaaa gtgtttaatg ccgataaaac gtactttgtt
     1201 ttaaacggca catcctcctc caacaaagtg gttttaaacg ccctgctgac accgggtgat
     1261 ctggtgctgt ttgaccgtaa taaccataag tctaaccacc acggagcgtt gctacaggct
     1321 ggtgcaacac cggtttatct ggaaacagca cgtaacccgt atggttttat cggtggcatt
     1381 gatgcgcact gcttcgaaga aagttatctg cgtgaactga ttacagaagt tgcaccgcaa
     1441 cgggcaaaag aggcgcgtcc gttccgcctc gctgtgattc agttaggcac ctacgacggt
     1501 actatttata acgcccgcca ggtggtggat aaaatcggcc atctgtgtga ctacatcctg
     1561 tttgactccg catgggtcgg ctatgaacag tttattccga tgatggcgga ctgttcgccg
     1621 ctgctactgg agcttaatga gaacgatccg ggtattctgg ttacgcaatc tgtgcataaa
     1681 caacaggctg gtttttctca gacttcacaa attcataaaa aagacagcca tatcaaaggg
     1741 caacagcgtt atgtaccgca caaacgcatg aacaacgcct ttatgatgca cgcctccacc
     1801 agcccgttct atccgctgtt tgccgcactg gatatcaacg ccaaaatgca tgaaggtgtc
     1861 agcggtcgta atatgtggat ggattgtgtg gttaacggta ttaatgcccg caaattgatc
     1921 ctcgataact gccagcacat tcgcccgttc gttcctgaac tggtggatgg taaaccctgg
     1981 cagtcgtatg aaacagcgca aattgcggtt gatctgcgct tctttaaatt tgtacccggt
     2041 gagcactggc attcttttga aggctacgca gagaaccaat actttgtcga tccgtgcaaa
     2101 ctgttgctga caactccagg tattgatgca cgtaacggcg aatatgaagc gttcggtgta
     2161 cccgcaacga ttcttgctaa cttcctgcgc gaaaatggcg tagtgccgga aaaatgcgat
     2221 cttaactcca tcctcttcct gctgactccg gcagaagata tggccaaact gcagcaactt
     2281 gttgccctgc tggtacgctt cgaaaaacta ctggaagctg atgcgcctct ggcggaagta
     2341 ctgccttcca tctacaaaca gcatgaagag cgctacgccg gttataccct gcgtcagttg
     2401 tgccaggaaa tgcatgattt gtatgcccgt cacaacgtga aacaactgca aaaagagatg
     2461 ttccgtaagg aacacttccc tcgcgtcagc atgaatccgc aagaagccaa ctacgcctac
     2521 ttacgcggtg aagtggagct ggttcgtctg ccggatgcag aaggccgtat cgctgccgaa
     2581 ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc ctggtgaaat ctggggtggt
     2641 gctgttctgc gttacttcag cgctctggaa gaaggcatca acctgctgcc aggttttgca
     2701 ccggagctgc agggtgtcta tatcgaagaa catgatggtc gtaagcaagt ttggtgctat
     2761 gtcatcaagc ctcgtgatgc gcaaagcgcc ctgttgaaag gggaaaaatt atgagtcagg
     2821 cgaaatcgaa caaaatgggt gtcgttcagt taaccatact gacgatggtc aacatgatgg
     2881 gctccggtat catcatgctg ccgacaaagc ttgccgaagt cgggacaatc tcaattatct
     2941 cctggctggt gacagccgta ggctcaatgg cactggcatg ggcattcgca aaatgcggta
     3001 tgttcagccg taaatcagga ggtatgggcg gttatgccga gtatgctttc ggtaaatccg
     3061 gtaactttat ggcgaactat acctacggcg tctcgctgct gattgctaac gtcgcgattg
     3121 ctatttcggc ggttggttac ggcaccgaac tgctcggcgc gagtttgtcg ccagtgcaga
     3181 ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt ggctaacttt ggtggtgcgc
     3241 gcattaccgg gcaaatcagt agcattaccg tgtggggggt cattattccg gtggtaggcc
     3301 tgtgcattat cggctggttc tggtttagcc cgacgcttta cgttgattcc tggaatccgc
     3361 atcatgcacc cttcttcagt gcggtaggtt cttccatcgc tatgacgctg tgggcttttc
     3421 ttggtctgga gtctgcgtgt gcgaatactg atgtagtgga aaacccggaa cgtaatgtgc
     3481 caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc tccaccaacg
     3541 tgattgccgg gattgtgcca aatatggagc tggcaaattc aacggcacca tttggtctgg
     3601 ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat tatggcgctg atggtgatgt
     3661 cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt aaatcttcat
     3721 ctgatgaagg ctacttccct aaaattttct cccgtgtaac caaagtggat gcaccggtgc
     3781 agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg attagcccgt
     3841 cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgactaat atcattccgt
     3901 atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg cccccctcaa
     3961 aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtacagcttt tatgcgctgt
     4021 actcatccgg ggaagaagcc atgctatacg gttccatcgt gaccttcctc ggttggacac
     4081 tgtatggtct ggtctcacca cgctttgaac tgaaaaataa acacggttaa ttaagcaaag
     4141 caaaaaaggg cgatcttccg accgcccttt ttcttatttg atatgtttac gcgtttttca
     4201 gaacttcgct aacaatttca accgcttctt tctcaatctg cttgcgatgt tcttcaccaa
     4261 ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc
     4321 agccgttgtc agtcatcact ttcagaccgc cgatagaagc gccattaccc ggtgccgccg
     4381 tcagacgcgc ggtgatcggg tcacctgcca gggtgctggc gctcaccatt tccggagaca
     4441 gcttagacaa cgccgctttt tgtgcggaag tcgctgctgc ctgcaaacgg ttatagctcg
     4501 gcgcaccaaa gcgttttgcc agttcgttat agtgttcctg cgggttctta ccggtgacag
     4561 cggtgatttc cgccgccagc agacacataa tgatgccgtc tttatcggtg gaccacggcg
     4621 tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc
     4681 cgtcgaacag accatcaaca aaccatttga aacctaccgg gacttctacc agcttacggc
     4741 ccaagtcatt gaccacacgg tcgatcatcg cagatgaaac cagcgtttta ccgacagcaa
     4801 catctttgcc ccactgcgga cgatgctgga acaggtaatt gatcgccacc gccaggtagt
     4861 ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt
     4921 tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc gcacactcgg
     4981 aggagcagtc catacggatc gcgccgtctt tatcaaggtg cataaagcgg aaggtttgat
     5041 cgacctgatc gttaacaata gtcaggttga ggttgtaata ctcgccaata cgcttccagt
     5101 attcgatacc ggaaccgccc agcggatcaa cgccaagcgt caggcccgct ttctgaatcg
     5161 ccgccatatc aacgatatcg gccagccctt ccacaaacgg ctgcaccaga tcctgctctt
     5221 tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat
     5281 cagccagcag tgcgtttgct ctgtcttcca ccactttagt gacgttggta tcagccgggc
     5341 caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa
     5401 tcacgatacc gtctgccagc gggccacctt ttttattgtg aaccagaatg gcattggaaa
     5461 tggcaggtgt cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg
     5521 ccagcacttc cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca
     5581 catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg
     5641 ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac
     5701 cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg
     5761 tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga
     5821 ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc
     5881 aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg
     5941 gtgttggtgt tggtgatcac ccaatacggc gtaccaggca catgtttcgg cttggtctga
     6001 ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc
     6061 aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc
     6121 agcataaagc gattgaccgc tcgcttttgc tctgcgtatt cgtccgaaag cagaagttca
     6181 cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt
     6241 gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggtaaatttc
     6301 aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata
     6361 tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg cagtgtctta
     6421 gctgaataca agtcgccagg agtatatgaa taaatcccgg cagcggatag cgtcaagcct
     6481 ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa
     6541 cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc
     6601 gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt
     6661 ggcagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc
     6721 caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg
     6781 atggcgcagg atcttgttga taccctggat gcacagcaga tcgacaaagc aacatttatc
     6841 ggtcactcca tgggcggtaa agcggtaatg gctcttactg cactagcccc tgatcgcatt
     6901 gacaagctgg tggcgattga tattgcgccg gtcgattatc acgttcgccg tcatgatgag
     6961 attttcgcgg ccatcaacgc agtcagtgaa tcagacgcac aaacacgcca gcaagcagcg
     7021 gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt
     7081 gttgacgggg agtggcgctt taacgtgcca gtattgtggg atcagtatcc gcatattgtg
     7141 ggttgggaga aaatcccggc atgggatcat cctaccctgt ttatccctgg cggcaattct
     7201 ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca ggctcgggcg
     7261 catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc
     7321 gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc
     7381 tttccgcgcg tggacgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat
     7441 ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca
     7501 aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac
     7561 taatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccaac taaaacgcga
     7621 caaagtacgt ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct
     7681 gaacgagctg gcggaatcgc gtaatatgag ccgtagcgaa ctgatcgaag agatgttaat
     7741 gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca
     7801 cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc
     7861 tttatccgtg ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca
     7921 tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa
     7981 tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca
     8041 aagaagatct ggaagcatat gacattctgc tgctgggcat cccaacctgg tattacggcg
     8101 aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat tttaatggta
     8161 aactggttgc gctgtttggt tgtggcgacc aggaagatta cgcagaatac ttctgcgatg
     8221 cattgggcac catccgcgac atcattgaac cgcgcggcgc aactatcgtt ggtcactggc
     8281 caaccgcggg ctaccacttc gaagcgtcaa aaggtctggc tgatgatgac cactttgtcg
     8341 gtctggctat cgacgaagat cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg
     8401 ttaaacaaat ttctgaagag ctgcatctcg acgaaattct caatgcctga tgtgatgcgg
     8461 cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat
     8521 ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt
     8581 aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt agttttcatt
     8641 taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc
     8701 taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc
     8761 taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg
     8821 agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg
     8881 aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg
     8941 tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc
     9001 acgatcacct gatctgcctc gactgcggta aggttatcga atttagtgat gattccatcg
     9061 aagcgcgtca gcgtgaaatc gccgcaaaac atggcattcg cctgactaac cacagtctct
     9121 atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga tgagcacgcg cacgaaggca
     9181 aataagccag cctgaacgag aaaagccaac ctgcaggttg gcttttttat acaagggaaa
     9241 taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaga
     9301 ccttatcaca atcggctttc accgcctgat catttccggt ttgcgttgcc tgaatgcact
     9361 gctgataatc cagtacgcgg acactttcct gctcggcaaa ctgctgatgt tgtttctctt
     9421 tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttccggcgaa ccttgcgcgg
     9481 tgttgataca ggcgctgtaa gcctctttca gacgactgtc ttcctgtggc gcggttgatt
     9541 gcgcacaagc caccagcccc gatgccatca tggcgatgag aatcagtttt ttcatagcaa
     9601 attcctcaaa cgtgcggcag gcacaaaacc tgccgcatcg ggcatcagaa gatggtgaat
     9661 ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttgcc ataaccaccg
     9721 ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg
     9781 cctttggcgc ggccgtcctg aatggtgtag accgcatcca ggctgtaggc agactcttca
     9841 atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtagc gggtttagca
     9901 tcccatgcgt aaacgtagga ggcgccgatg gcaaaacctg gaagattcca gtttttcagg
     9961 tcatacatcg cgccgaagaa gaccgctttt tcgccgttgg cgttgaagtc agaacggtta
    10021 tcccaccaga tatccaggcg accgttcgag gaagcgtagg ttggtgtcat acgttgcagg
    10081 aagtatccct gctgaccgtc ggctttaacc caggtgcctt ccagacgcaa atccactacg
    10141 tcagccgccc ggtaaccaaa ggtcaacgcc tgcagccagg cggtgccgtc ataaagatcg
    10201 ttggcgctgc gatcgtcaac tttatcgcgc gtgccataga actggtagct ggtggttaac
    10261 gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc gatataccct
    10321 tcggcctgac caaacgccgc ttccagtacg aagttatttt tgaagtcgta cttcgcccca
    10381 agggagtgta gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc
    10441 agatgccacg gtgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca
    10501 tcgccgtaat caaaattcgc cccggcctcc gccccctgat aagtacccgg cataaagctc
    10561 cagtgtggcg ctaacagcgt ctgaccggtt ggctgaatat aacctgccct cgcccaaacc
    10621 ggaccgtatt taaatttggc cgctgcttta tacaggctta taccgctttt atcgccggac
    10681 cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgagagctg
    10741 tcgccgtttt ccgccatttc aatcgccgta aacgcggcaa tatcaaggcc gaacatatca
    10801 gcagcataac cagactgaaa atcgaggttg gcgttccagg tggagtgaga aaggttggtt
    10861 ttgtatttat cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatagata
    10921 ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg aattgccata
    10981 aagcccgaca tagctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta
    11041 aacgcacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg
    11101 aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac
    11161 tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca
    11221 accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcagttga aaggcttgca
    11281 gaagaataaa aacgtg
//
LOCUS       DAJOTB010000145        11240 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJOTB010000145 DAJOTB010000000
VERSION     DAJOTB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.145"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            221..2644
                     /gene="pgaA"
                     /locus_tag="OHN28_003902"
     CDS             221..2644
                     /gene="pgaA"
                     /locus_tag="OHN28_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HCQ2299206.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2653..4671
                     /gene="pgaB"
                     /locus_tag="OHN28_003903"
     CDS             2653..4671
                     /gene="pgaB"
                     /locus_tag="OHN28_003903"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCQ2299207.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4664..5989
                     /gene="pgaC"
                     /locus_tag="OHN28_003904"
     CDS             4664..5989
                     /gene="pgaC"
                     /locus_tag="OHN28_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCQ2299208.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5991..6404
                     /gene="pgaD"
                     /locus_tag="OHN28_003905"
     CDS             5991..6404
                     /gene="pgaD"
                     /locus_tag="OHN28_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCQ2299209.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6454..7242)
                     /gene="phoH"
                     /locus_tag="OHN28_003906"
     CDS             complement(6454..7242)
                     /gene="phoH"
                     /locus_tag="OHN28_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCQ2299210.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7869..9140)
                     /gene="efeB"
                     /locus_tag="OHN28_003907"
     CDS             complement(7869..9140)
                     /gene="efeB"
                     /locus_tag="OHN28_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCQ2299211.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9146..10273)
                     /gene="efeO"
                     /locus_tag="OHN28_003908"
     CDS             complement(9146..10273)
                     /gene="efeO"
                     /locus_tag="OHN28_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCQ2299212.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10331..11161)
                     /locus_tag="OHN28_003909"
     CDS             complement(10331..11161)
                     /locus_tag="OHN28_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCQ2299213.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
BASE COUNT         2951 a         2775 c         2638 g         2876 t
ORIGIN      
        1 atgccgtatt cctgaagatc ctcatcattg gaatggattt tcgggcgaga aaaggatttt
       61 atatggacac tctgctcatc atttcttctt ctcatcatca acaattcacg tctctcttcc
      121 gcgtttaata acggattatg agatgcaaaa atatctttct tttcagttac ctgtaattag
      181 atacagagag agattttggc aatacatgga gtaatacagg atgtattcaa gtagcagaaa
      241 aaggtgcccg aaaaccaaat gggctttgaa aattcttact gctgcatttt tagcagcgag
      301 tcccgcggcg aagagtgctg ttaataacgc ctatgatgca ttgattattg aagctcgcaa
      361 aggaaatact cagccagctt tgtcatggtt tgcacaaaaa tcaacactca gtaataacca
      421 aattgctgac tggttacaga ttgccttatg ggccgggcaa gataaacagg ttattaccgt
      481 ttacaaccgc tatcgtcatc agcaattacc agcgcgtggt tatgcagctg tcgccgtcgc
      541 ttatcgtaac ctgcaacaat ggcaaaactc gcttacactg tggcaaaagg cgctctctct
      601 ggagccgcaa aataaggatt atcaacgggg acaaatttta accctggcag atgcgggtca
      661 ctatgatact gcgctgttta aacttaagca gcttaaatct ggagcaccgg acaaagccaa
      721 tttactcgca gaagcctata tctataaact gacggggcgt tatcaggatg aattacgggc
      781 gatgacagag tcattacctg aaaatgcatc taagcaacaa tatcccacag aatacgtgct
      841 ggcattacgt aataatcaac tagctgccgc gattgacgat gccaatttaa cgccagatat
      901 tcgcgctgat attcatgccg aactggtcag actgtcgttt atgcctacgc gcagtgaaaa
      961 tgaacgttat gccattgccg atcgcgccct cgcccaatac gctgcattag aaattctgtg
     1021 gcacgataac ccagaccgca ctgcccagta ccagcgtatt caggttgatc atcttggcgc
     1081 gttattaact cgcgatcgtt atagagacgt tatttctcac tatcagcgtt taaaaaagac
     1141 ggggcaaatt attccgccct gggcgctata ttgggttgcg tcggcttatc tcaaagatca
     1201 gcagccgaaa aaagcacagt caataatgac cgagctcttt tataacaagg aaaccattgc
     1261 cacggattta tccgatgaag aacttgcgga tctcttttac agccacctgg agagtgaaaa
     1321 ttatcccggc gcgctaactg tcaccaaaca taccattaat acttcgccgc ctttccttcg
     1381 gctaatgggc acgcctacga gcatcccgaa tgatacctgg ttacaggggc attcgtttct
     1441 ctcaaccgta gcaaaatata gtaatgatct tcctcaagct gaaataacaa ccagagaact
     1501 tgcttataac gcaccaggaa atcagggact gcgcatcgat tacgcgagtg tgttacaagc
     1561 ccgcggttgg cctcgtgcgg cagaaaatga attaaaaaaa gcagaagtga tcgagccacg
     1621 taatattaat ctggaggttg aacaagcctg gacagcatta acgttacaag aatggcagca
     1681 ggcagctgtc ttaacccacg atgttgtcga acgtgaaccg caagatcccg gcgttgtacg
     1741 attaaaacgt gcggttgatg tacataatct tgcagagctt cgtatcgctg gctcaacagg
     1801 aattgatgcc gaaggtccag atagtggcaa acatgatgtc gacttaacca ccatcgttta
     1861 ttcaccaccg ctgaatgata actggcgcgg ttttgctgga ttcggttatg ccgatggaca
     1921 atttagcgaa ggaaaaggga ttgttcgcga ctggctggca ggtgttgagt ggcggtcacg
     1981 taatatctgg ctcgaggcgg agtacgctga acgctttttc aatcatgagc ataaacccgg
     2041 cgcgcgcctg tctggctggt atgattttaa tgataactgg cgtattggtt cgcaactgga
     2101 acgcctctct caccgcgttc cattacgggc aatgaaaaat ggtgttacag gcaacagcgc
     2161 acaggcttat gttcgctggt atcaaaatga gcggcgtaag tacggtgtct cctgggcttt
     2221 cactgatttt tccgacagta accagcgtca tgaagtctca attgagggtc aagaacgcat
     2281 ctggtcttca ccatatttga ttgtcgattt cctacccaat ctgtattacg aacaaaatac
     2341 agaacacgac accccatact acaaccctat aaaaatgttc gatattgttc cggcatttga
     2401 ggcaagtcat ttgttatggc gaagctatga aaatagctgg gagcaaatat tcagcgcagg
     2461 tgttggtgcc tcctggcaaa aacattatgg cacggatgtc gtcacccaac tcggctacgg
     2521 gcaacgtatt agctggaatg acgtgattga tgctggcgca acgctacgct gggaaaaacg
     2581 accttatgac ggtgacagag aacacaactt atacgttgaa ttcgatatga cattcagatt
     2641 ttaaggataa atatgttacg taatggaaat aaatatctcc tgatgctggt gagtataatt
     2701 atgctcaccg cgtgcattag ccagtcaaga acatcattta taccgccaca ggatcgcgaa
     2761 tctttactcg ccgagcaacc gtggccacat aatggttttg tggtgatttc atggcataac
     2821 gttgaagacg aagctgccga ccagcgtttt atgtcagtgc gaacatcagc actgcgtgaa
     2881 caatttgcct ggctgcgcga gaacggttat caaccggtca gtattgctca aattcgtgaa
     2941 gcacatcgag gaggaaaacc gctaccggaa aaagcagtag tgctgacttt tgatgacggc
     3001 taccagagtt tttatacccg tgtcttccca attcttcagg ccttccagtg gccggctgta
     3061 tgggctcccg tcggcagttg ggtcgatact ccagcggata aacaagtgaa atttggcgat
     3121 gagatggtca gtcgagaata ttttgccacg tggcaacaag tgcgagaagt tgcgcgttcc
     3181 cggctcgttg aggtcgcttc tcatacatgg gattctcact acggtattca ggctaatgcc
     3241 accggcagct tattgcctgc atatgtaaat cgtgcatatt ttactgacca cgcacggtat
     3301 gaaaccgcag cagaataccg ggaaagaatt cgcctggatg ctgtaaaaat gacggaatac
     3361 ctgcgtacaa aggctgaggt aaatccacac gtttttgttt ggccttatgg cgaagcgaat
     3421 ggcatagcaa tagcggaatt aaaaaaactc ggttatgaca tgttcttcac ccttgaatca
     3481 ggtttggcaa atgcgtcgca attagattcc attccgcgag tattaatcgc caataatccc
     3541 tcattaaaag agtttgccca gcaaattatt accgtacagg aaaaaccact acaacgggta
     3601 atgcatatcg atcttgatta cgtttatgac gaaaaccgcc agcaaatgga tcgcaatatt
     3661 gatgtgctaa ttcagcgggt aaaagatatg caaatatcta ccgtgtattt gcaagcattt
     3721 gctgatcccg atggtgatgg gctggttaaa gaggtctggt ttccaaatcg tttgctacca
     3781 atgaaagcag atatttttag tcgggttgcc tggcaattgc gtacccgctc aggtgtaaac
     3841 atctatgcgt ggatgccggt attaagctgg gatttagatc ccacattaac gcgagtaaaa
     3901 tacttaccaa ctggggagaa aaaagcacaa attcatcctg aacaataccg ccgtctctct
     3961 cctttcgatg acagggtcag agcacaagtt ggtatgttat atgatgatct tgccggacat
     4021 gctgcttttg atggcatatt gttccacgat gatgctttgc tttcagatta tgaagatgcc
     4081 agtgcagcgg ctatcgcggc ttatcagcaa gctggcttta gcgggagtct gagcgaaatt
     4141 cgacaaaacc cggagcaatt taaacagtgg acccgcttta aaagtcgtgc gttagctgac
     4201 ttcactttag aacttagtgc gcgcgtaaaa gccattcgtg gtccacatgt taaaactgca
     4261 cgaaatattt ttgcacttcc ggtaatacaa cctgaaagtg aagcctggtt tgcacagaat
     4321 tatgctgatt tcctaaaaag ctatgactgg accgctatta tggctatgcc ttatatggaa
     4381 ggcgtcacag aaaaatcggc tgatcaatgg ttaatacaat tgaccaatca gatgaaaagc
     4441 atcccgcagg ctaaagacaa atcaatttta gaattacagg cacaaaactg gcagaaaaat
     4501 ggtcagcatc aggctatttc ttcacaacaa ctcgctcact ggatgagcct attacaactg
     4561 aatggagtga aaaattatgg ttattatccc gacaattttc tgcataacca acctgaaata
     4621 gacgttattc gtcctgagtt ttcaacagcc tggtatccga aaaatgatta atcgcatcgt
     4681 atcgtttttt atattatgtc tggtgttatg cataccccta tgcgtagcgt actttcactc
     4741 tggtgaactg atgatgaggt tcgttttctt ctggccgttt tttatgtcca ttatgtggat
     4801 tattggcggc gtctatttct gggtctatcg tgaacgccac tggccgtggg gagaaaacgc
     4861 accagccccc cagttgaaag ataatccatc tatctccatt atcattccct gttttaatga
     4921 ggagaaaaac gttgaagaaa ccatacatgc cgctttagca cagcgttatg agaacattga
     4981 agttattgcc gtaaatgatg gttcaacaga caaaacccgt gccatcctgg atcgcatggc
     5041 tgcacaaatt ccccgtctgc gggtcattca tctggcgcaa aaccagggga aagccattgc
     5101 gcttaaaacc ggagctgccg cggcgaaaag tgaatatctg gtgtgcattg atggcgacgc
     5161 gttattagac cgcgatgcag cggcatatat tgtggaaccg atgttgtaca acccgcgtgt
     5221 gggtgccgta accggtaatc ctcgtattcg cacacgttct accctggtgg gtaaaattca
     5281 ggttggcgag tattcctcaa ttattggttt gatcaagcgg acccagcgta tctatggaaa
     5341 cgtatttacc gtttccggtg ttattgccgc atttcgtcgc agcgccctgg cagaagtggg
     5401 ttactggagt gacgatatga tcaccgaaga tattgatatt agctggaagc tgcagttgaa
     5461 tcagtggacg attttttacg agccacgggc gctgtgctgg atattaatgc ctgaaacgtt
     5521 aaaagggctg tggaatcagc gcctgcgctg ggctcagggc ggtgcagaag tattcctcaa
     5581 aaatatgaca aggttgtggc gcaaagaaaa ctttcgaatg tggccactgt tttttgaata
     5641 ctgtctgacg acaatatggg cctttacctg cctggtcggt ttcattattt acgcagccca
     5701 acttgcaggt gtaccgttaa atattgaatt gacacatatc gcagcgacac atactgccgg
     5761 aatattattg tgtacgttat gtttactgca atttattgtc agtctggtga ttgagaatcg
     5821 ctatgagcac aatctggctt catcgctttt ctggattatt tggttcccgg ttattttctg
     5881 gatgctaagc ctggcaacga cgttggtatc atttacacga gtcatgttga tgcctaaaaa
     5941 acaacgcgct cgttgggtaa gtcccgatcg tgggattctg agaggttaat atgaacaatc
     6001 taattattac gacccgacaa tcgccagtac gtttactggt tgattatgtt gccacaacta
     6061 tcttgtggac attatttgct ttattcatat tcttattcgc cattgatctg ctgacgggtt
     6121 atttctggca aagcgaggcc agaagccgac ttcagttcta ttttttgctg gcagtggtga
     6181 atgccgtcgt gttaatcgtc tgggcgttgt acaataagct gcgttttcaa aaacagcagc
     6241 atcatgcagc ataccaatat acgccgcaag aatatgcaga gagcttagca atacctgatg
     6301 agctttatca gcaactacaa aaaagccaca ggatgagcgt acatttcacc agccagggac
     6361 aaataaaaat ggtggtttca gaaaaaacgc tagtccgggt ataaacaccc aaaacaaagc
     6421 ccggttcgcc cgggctctgc accgataata cacttaactg tatgcatgta gcgtacgttg
     6481 gcaaagtgcc gaacgtacgc aatcctcttt accgaatcgg acgatcccga ccatttcatc
     6541 ctcttcgaaa cgttccagcg cgtcacttaa tccggagcgc acgccgcgag gcaaatcgca
     6601 ttgcgtgata tcaccgttga cgataaccgt cacgttctcc ccgaggcggg ttaaaaacat
     6661 tttcatttgc gcggcagtca cattctgcgc ctcgtcaaga atgacgactg cattttcaaa
     6721 ggtacgtcca cgcatatagg cgaacggcgc aatttccacc ttccctattt ccggtcgcag
     6781 gcagtactgc ataaaggaag cccctaagcg acggaccagc acgtcgtaga ccgggcgaaa
     6841 atagggagca aacttttctg cgatatcacc aggtaagaaa ccaagatctt catcggcttg
     6901 cagaactgga cgggtgacga taatcctgtc aacatcctta tgtatcaggg cctctgccgc
     6961 ttttgctgcg ctgatccagg tttttccgca cccggcctca cccgtggcaa atatcagctg
     7021 cttactctca atagccttca gatagtgcaa ttgcgcttca tttcgcgcga ggatgggcga
     7081 ggtatcgcga ctgtcgcggg ccataccaat ggcttctacg ccgctcattt gcacaagcga
     7141 ggtgaccgat tcttcttcac gctgcttatg gctgcgcgaa tcccgtctca gcacacgttt
     7201 tgcctcgcga cgagctttga tcactgcttt ttgtcttccc atggagagca ccttgagttg
     7261 tttgtattca tcacacgcgc cgttggcagc gcgattatgc gcacgaacat cagagggttg
     7321 gcttccttgt aagccatagt ttgcttttgg ataaaatgcc gaaaaacggc tacgcgcacc
     7381 gtttacggcg tcggtaacac atgaaaagaa aggatgaggt tgaaaataca aagtgacgag
     7441 atgactaccg gaggagaaat ctccgcgagt ggtggcgcgc tgattctcta aaacatgtcc
     7501 agtacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     7561 cgctgctact taataagtac aacagatttc gcattcattg caacaatata ttcacttata
     7621 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat
     7681 gacagtgatg aaaaaagctg tgtgctttca gcaggattcg cggtactggt aaaagcttgc
     7741 cggatgcggc gtgaacgcct tatccggcct accgacccgc accgattgta ggcatgataa
     7801 gacgcgcaag cgtcgcatca gcgcacgaac gcctggtgcg gcgtaaacgc cttatacgcc
     7861 taaaaacatt aaacccgcaa taatgcgctt ccgagataat cgttcgcgtc cttcacaccc
     7921 ggcagcgcaa agaaatagcc gccaccgata ggtttgacat attcctccag cgcctcgcca
     7981 ttcaaccttt tttgtactgt caggaagccc ttttccagat cgtgttggta gcagacaaac
     8041 agtagcccca tatcgagttg cccggaattg gtgacgccca acgaataact gtagccacga
     8101 cgcagcatca ggctggactc gctctccgcc gtgcggggat tcgccagccg gatatggctg
     8161 tccagcgcaa tcaccttccc ttccggatcg ctagcgtaat cgggaacatc atgctcatgc
     8221 tgcatgccca gcggcgcacc ggtttgctta tcacgaccaa atatcgtctg ctgctctttc
     8281 agcggcgttc tgtcccaaaa ttccactcga aactgaatca agcgtactgc ctgatagctg
     8341 ccaccgattg tccacgcagg ctcctgctga tctgccgtca cccacaccac tttttgcatc
     8401 aacttcgcat tctggctatc gggattggcg gtgccgtctt taaagcccag caaatttatc
     8461 ggcgtctctt tgcctttact acgcgctgcg tggtcggaaa taaacccttc ccgcttccag
     8521 cgcacgctga gcaaatccgg cgtgtgtttg atgatatcgc gcagcgcatg gatcaccgtg
     8581 tcctgggtgt tggcgcaaat ttgtagcaac acatcaccat gacataacgc cgcatccagc
     8641 gagtcgttgg ggaaacgcgt catcttctgc aacttttttg gcatctgtgg cgcaaggcca
     8701 aagcgctcat caaacaatga gtgacccacc gataacgtga tggtgagatt atcgggcgca
     8761 atgtagccgc caagaatgcc ggaatcgagt ggtggcaggc gcggatttgg cgtttctggt
     8821 gccgctccgc cctgagtcag aaaagcaaaa cgctgagtca acaagcgaaa caaccgttca
     8881 agatcggctt tatcgctggc aagcacatca aacgccacca gcatcattgc agcctgttgt
     8941 ggtgtcagga tccctgcctg atgctcacca taaaacggct gtttctcatt gcgtgcatcc
     9001 ggtgaaagcg tacccggcgc actttgcgtt ttttgtgcat gagcaaccgg acaacttccc
     9061 gccagcgcca gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg
     9121 ttttcatctt catactgcat aacgcttagt ccagccccag cacaccacga agttgcgcca
     9181 gatcttccgc cagcgcagta atcggtcctt tcagtgcgtt ccggtcggca tcggtcagtt
     9241 tgtcgtaatt ttcaaaaccc tctttagtgc ggtatttcgc cagaatggta tcgacctttt
     9301 tgaagttggc atcgactttt gccaacagtt ccggattcgc tttttgtagt tgtggacgca
     9361 gcagatcgac aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt
     9421 ggctgtagcg atcttcttca ccgctgattt tgctggccgc cacttcctca atcagtccgg
     9481 ctgcaccgcc gaccactttt gaaggaggga aagccagttc actgatgcgt ttttgcaaat
     9541 cgaccacatc ggtataaagc tggtcagcat acttatccat ccctttggtg gtgttatcgc
     9601 caaacaatgc tttttccaga cggtggaaac cggtgaattt tggatcggcg gctttttgct
     9661 cgtaatcatc ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg
     9721 gttcgatgcg ctcatagtgc tgccgcgtcg gcgcatacag tgctttcgct ttttcgatat
     9781 cgcctgcttt aatagcgtcg gtaaaggctt tggtgtcggt caccagttgc gtggtttccg
     9841 ccatgacata cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt
     9901 gcgccgcatc cgccgttgct tcacctttga cgatcaactt ccctttcggg ttagtcagca
     9961 gaccgcaggt catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac
    10021 caggggcgat attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca
    10081 gcgccttctg gctgtggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg
    10141 tcatcggctc gcactgctta tctgtcacag tgactttgac ttgcggaata tcggcggcgt
    10201 tagccataaa agcagaagag aacagcgcag ccacgctcaa ctgcaatgcg ttacgacgga
    10261 agttaatggt catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc
    10321 gtcgtatttg ctacacggag cgggacgcag tcgcccctgc gcgtggtggc agaacaaatg
    10381 ccaccagcgc cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt
    10441 cctgatagcc aaaaatccct tccatcagtg tgccaaacag cgagtgagtt gagagcaccg
    10501 cgctcatatc gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc
    10561 gaatggcacc agctgccagc cctgcggcga cgaagagaat aaacagactg gtccatttaa
    10621 aaaatgcacc aagattgagg cgaataccgc cccagtagag caggaagcca agcaccacgg
    10681 ctgtagcaag accgagcatt gcacccagcg gcggccagac cccgacatct tgttgaaatg
    10741 ccgccagcag gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca
    10801 tcaccagcgc ccagccatga tgatttccac gctgcaatgc gctatcgacc gcctgctcca
    10861 gttgtacttt gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca
    10921 ccacggcgat caccgccaca atgccttcaa acagttcctg ttctttttgc ggaaattcgc
    10981 cggtggtttc gttaatgaag atacccaagc ccaggcacaa cgcagcggca agcaacacgc
    11041 caatccacat tacaccaatc catcggcctc gctgggtacg cttaagatag ctggcaatca
    11101 aactgacaat cagcgcggct tcaagtcctt cgcgcaacat aatgagaaac ggaacaaaca
    11161 tgccacccac ccttaaacgt ttttcttagt caatctatgc aaagaaaagt aaatcgcagt
    11221 gatagcgatt atcattacga
//
LOCUS       DAJOTB010000146        11163 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJOTB010000146 DAJOTB010000000
VERSION     DAJOTB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.146"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            27..350
                     /locus_tag="OHN28_003910"
     CDS             27..350
                     /locus_tag="OHN28_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCQ2299214.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
     gene            complement(470..649)
                     /locus_tag="OHN28_003911"
     CDS             complement(470..649)
                     /locus_tag="OHN28_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCQ2299215.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            723..2084
                     /gene="pabB"
                     /locus_tag="OHN28_003912"
     CDS             723..2084
                     /gene="pabB"
                     /locus_tag="OHN28_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCQ2299216.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSHLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLLEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2088..2666
                     /locus_tag="OHN28_003913"
     CDS             2088..2666
                     /locus_tag="OHN28_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCQ2299217.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEGEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2850..4214
                     /gene="sdaA"
                     /locus_tag="OHN28_003914"
     CDS             2850..4214
                     /gene="sdaA"
                     /locus_tag="OHN28_003914"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2299218.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4345..5943
                     /locus_tag="OHN28_003915"
     CDS             4345..5943
                     /locus_tag="OHN28_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2299219.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(5947..7503)
                     /gene="yoaE"
                     /locus_tag="OHN28_003916"
     CDS             complement(5947..7503)
                     /gene="yoaE"
                     /locus_tag="OHN28_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCQ2299220.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7967..8938
                     /gene="manX"
                     /locus_tag="OHN28_003917"
     CDS             7967..8938
                     /gene="manX"
                     /locus_tag="OHN28_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCQ2299221.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9001..9801
                     /gene="manY"
                     /locus_tag="OHN28_003918"
     CDS             9001..9801
                     /gene="manY"
                     /locus_tag="OHN28_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCQ2299222.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9814..10665
                     /gene="manZ"
                     /locus_tag="OHN28_003919"
     CDS             9814..10665
                     /gene="manZ"
                     /locus_tag="OHN28_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCQ2299223.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10720..>11163
                     /locus_tag="OHN28_003920"
     CDS             10720..>11163
                     /locus_tag="OHN28_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCQ2299224.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKVFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2725 a         2830 c         2821 g         2787 t
ORIGIN      
        1 aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat atgcctgctg
       61 taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt
      121 cgatgaatga aagtacggcg aagggaatct ttaaatattt aaaagaactg ggagtacccg
      181 cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct ggtttcacgg
      241 aaaaaatggt tggatgggca aaaaaaatgg agtctggtga acgctctgtg attaaaaatc
      301 ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtttga gttaaattca
      361 catcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac
      421 aatcaaataa tcatcggctt ctataacgaa agcgatacgg attagtgtgt tactcgtctt
      481 catcctcaaa acgcgccacg attcgctcgc cggaatggtt ggcacgcagc tcttctgcca
      541 ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga
      601 cggctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct
      661 gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac
      721 tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat
      781 tttatttctc ccacttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg
      841 atcatccgta tagccgcttt gatattgtgg tcgccgagcc aatttgcact ttaaccactt
      901 tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc
      961 tacaggtgct ccagcaggtg ctggatcgtg cagacattcg cccagcgcat aacgaagatt
     1021 tgccatttca gggcggcgcg ctggggttgt ttggctacga tctgggccgc cgttttgagt
     1081 cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg
     1141 actgggcgct ggttgtcgac caccagcgtc aaacagtttc tttgctgagt cataatgatg
     1201 tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc cccgcaggaa gatttcacac
     1261 tcacttccga ctggcaatcc aatatgaccc gcgaacagta cggcgaaaaa tttcgccagg
     1321 tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg
     1381 cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc
     1441 cgtttagcgc ttttttacgt cttgaacagg ctgctatttt aagcctttca ccagagcggt
     1501 ttattctttg tgataatggt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc
     1561 tgcccgatct tctggaagat agcaaacaag cagaaaaact ggcgaactca acgaaagatc
     1621 gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag
     1681 caggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc gtgcatcatc
     1741 tggtcagcac cataacggcg caactaccag aacagttaca cgccagcgat ctgctgcgcg
     1801 cggcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg
     1861 acgaactgga accgcagcga cgcaatgcct ggtgcggcag cattggctat ttgagctttt
     1921 gcggcaacat ggacaccagc attactatcc gcacgctgac taccattaac ggacaaattt
     1981 actgctctgc gggcggtgga attgttgccg atagccagga agaagcggaa tatcaggaaa
     2041 cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta
     2101 gcctgacgct tgatgatttt ttatcgcgct ttcaactttt gcgcccgcaa attaaccggg
     2161 aaaccttaaa tcatcgtcag gctgccgtgt taatccccat cgtccgccga ccgcaaccgg
     2221 ggttgttgct gactcagcgt tcgattcatc tgcgtaaaca cgctggacaa gtggcattcc
     2281 ctggaggcgc agtcgatgac acggacgcat cagttatcgc cgctgcgctg cgcgaagctg
     2341 aagaagaggt cgctataccg ccttccgccg ttgaggttat cggcgtgctg ccgcccgtcg
     2401 atagcgtcac tggctaccag gtaaccccag tggtcggcat tatcccgccc gatctgccgt
     2461 atcgcgccag tgaaggtgaa gtctccgccg tctttgaaat gccgctcgcc caggcattac
     2521 atctgggtcg ttatcatcct ttagatatct accgccgtgg cgattcacat cgggtatggc
     2581 tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg
     2641 cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt
     2701 gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat
     2761 agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga
     2821 agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg
     2881 ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg
     2941 atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg
     3001 gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg
     3061 caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag
     3121 aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca
     3181 acgggatgcg ttttcataac ggtaacctgc cgctgcatga aaacggtatg caaatccacg
     3241 cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta
     3301 tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt
     3361 tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggcttttcg ctgtctggtc
     3421 tcgctatgca aaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc
     3481 atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaataccgaa ggcgtactgc
     3541 caggcccgtt gcgcgtgcca cgtcgtgcgt cggccctgcg ccggatgctg gtttccagcg
     3601 ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag
     3661 ttaacgaaga aaacgccgcc ggtggccgtg tggtaactgc gccaactaac ggtgcctgcg
     3721 gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcagtt agcccagaca
     3781 tctatacccg ttactttatg gcagcgggcg cgattggtgc actgtataaa atgaacgcgt
     3841 ctatttccgg tgcggaagtc ggttgccagg gcgaagtagg tgttgcctgt tcaatggctg
     3901 ctgcgggcct tgccgagctg ttgggcggta gcccggaaca ggtttgtgtg gcagcggaaa
     3961 ttggcatgga acacaacctc ggtctgacct gcgaccccgt tgcagggcaa gttcaggtgc
     4021 cgtgcattga acgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc
     4081 tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa
     4141 ccggtaagga catgaacgct aaataccgcg aaacctcacg cggtggtctg gcaatcaaag
     4201 tccagtgtga ctaatacttc ttattcgccc atctgcaacg gatgggcgaa tttattcccg
     4261 ctttctcgtc tgctgtaatt ttccccacta cacttccact gttgcgtcag gcgtttgtcg
     4321 ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc
     4381 cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc
     4441 gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac
     4501 gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt
     4561 ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa
     4621 ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg ctccagcatt
     4681 tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca
     4741 cttttacgac tgacgaccga ccgtgccctg ataaaaggca gtccggtttt gattcaatgg
     4801 acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg
     4861 acggcaatgc tccttgaacc acaactgccg caaatcagta gcgccagcct gacggtggac
     4921 agtcggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaaaacaat
     4981 gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggcccgggg
     5041 gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt
     5101 ttactggtgg gctacatcgc ctggctggcg accgcttacc gaatgagctt ttcccgcgag
     5161 atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg
     5221 cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc
     5281 tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg
     5341 acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccaat gagtagtcag
     5401 tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat
     5461 ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa
     5521 cgcgatgcct tactggatgt tgattaccgg attgcccgcg agctgcatcg taaaaacgtc
     5581 aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta
     5641 cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt
     5701 aactcgacgg tgaccgatat catcatcgca ctggggcaaa gactgaatat tgaactggtg
     5761 gcggagggcg tagaaacgca agaacaggcg aagtatttgc gcagtcatgg cgtgcatatt
     5821 ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg
     5881 ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccgttat gccgttacgt
     5941 taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac
     6001 acgataatca ttggcttcaa tgatggtgat atgcagtgga cctacatcaa tcacatcgcc
     6061 cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg cgatatcatc
     6121 gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc
     6181 ttttaccagc cagccgtcac cgtcggtaat gatttccggc gtttcgtcag cgtccgggaa
     6241 ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta ccacaccaaa
     6301 ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca gcaggttgat
     6361 cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagctatcg ccgccacatc
     6421 aactccctct tccagcgcca ccagcagttc tttagcacgt acgatgccga taatttcatc
     6481 cagttcaccg cgacataccg ggaacaaact gtgcggtgag gagagcagtt gctcgcggat
     6541 ttcatcgacc ccgagattag catcaaccca gcttatttca ccgcgcggcg tcatgatccc
     6601 gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc
     6661 aaacgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct gaacattggc
     6721 ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa
     6781 agtcgactgg tgacgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat
     6841 cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc
     6901 caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg
     6961 ctggttaaca aatcgcgtca gcggtttcga tgccagcagc ataaccgcca tcgcaatcac
     7021 taccgccgcc atcatcaccg gcaaatggtt aaccatccct actgcagtaa ttaccgcatc
     7081 caacgaaaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta
     7141 gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca gttcggttgt
     7201 tgctttgaac agcaagaata tgcccccgaa caacataatc aggtcgcgtc cggagaagga
     7261 gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag
     7321 cagccccaga cgcataatca gcgccagtga taatcccagc aaacgcgctt tatcgcgttg
     7381 ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac
     7441 aatttcgaga acaacaagcg tgagtagccc cgcccaaatc gaggggtcca ttaagaattc
     7501 catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg
     7561 acaaaatgca aacgaaaggt gcggcataaa gtgccagaaa gacaggcgtt aaaaggcctg
     7621 atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatggtt
     7681 gttaaacagg agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt
     7741 tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa
     7801 ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc gcgaaacgca
     7861 gggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac gattcaaaaa
     7921 tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga ccattgctat
     7981 tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag aaatgctgtt
     8041 aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg ccgaaacgct
     8101 gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg tgctgtttct
     8161 cgttgataca tggggaggca gcccattcaa tgctgccagc cgcattgtcg tcgacaaaga
     8221 gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt taatggcccg
     8281 agatgatgac cctagctttg atgaactggt ggcactggca gtagaaacag gccgtgaagg
     8341 cgtgaaagca ctgaaagcca aaccgattga aaaagccgcg ccagcacccg ctgccgcagc
     8401 acccaaagtg gctccaactc cggcaaaacc aatgggaccg aacgactaca tggttattgg
     8461 ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct ggaccaaaga
     8521 aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata ccgttcgtaa
     8581 gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg atgttgccaa
     8641 aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgcgtaatgc tgttatttac
     8701 caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct ctgttaacgt
     8761 cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt cggttgatga
     8821 aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt attgagctgg aagtccgtaa
     8881 ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg ataagtaacg
     8941 tattgtgttg attatcactc agttttcaca cttaagtctt acgtaaacag gagaagtaca
     9001 atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat cgcaggtatg
     9061 ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac cctggtgggt
     9121 atcgttcttg gcgatatgaa aaccggtatt attatcggtg gtacgctgga aatgatcgcg
     9181 ctgggctgga tgaacatcgg tgctgccgtt gcgcctgacg ccgctctggc ttccatcatt
     9241 tctactattc tggttatcgc aggtcatcag agcattggtg caggtatcgc actggcaatc
     9301 cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactatcac cgttgctttc
     9361 cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc ctggatccac
     9421 gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat cgttgcgctg
     9481 tctgttggta ccagcgaagt ccagaacatg ctgaatgcga ttccggaagt ggtgaccaat
     9541 ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt tatcaacatg
     9601 atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc agcattcacc
     9661 aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct ctacatccaa
     9721 cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggctgctgg taacaacgat
     9781 ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca aactaccacc
     9841 gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa cctctttcag
     9901 ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat ggtaccggca
     9961 attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg ccgtcacctg
    10021 gagttcttta acacccagcc attcgtggct gcgccgattc tcggcgtaac cctggcactg
    10081 gaagaacagc gtgctaatgg cgcagagatc gatgacggtg ctatcaacgg tatcaaagtc
    10141 ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac ggtacgtccg
    10201 gtatttgcag cgctgggggc tggtatcgcg atgagcggca gcctgttagg tccgctgctg
    10261 ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt agcgtatggt
    10321 tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca aaaactgacg
    10381 gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaataa gtggacacat
    10441 gtcaacatcc cgctggtggt ctctcgcatt actgaccaga cgggcaaaga acacgttacc
    10501 actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct gctgaccttt
    10561 gcttgtatgt ggctgttgcg caaaaaagtt aacccgctgt ggatcatcgt tggcttcttc
    10621 gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg ttgtacacta
    10681 ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac ggacctggta
    10741 ctgattcttt tcatcgccgc acttctggcc ttcgcgatct acgatcagtt catcatgccc
    10801 cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg catcgatagc
    10861 gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa tcatggcgcg
    10921 cttataacaa catggttatt aagtgcactg gctctgatgg gtttttatat attctggatt
    10981 cgcgttccga agatcatctt taaacaaaaa gtttttttct tcgccaatgt ctggattgaa
    11041 tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat gcaattagaa
    11101 cagcgtcggc tgttaatccg cgttcgaaat atcgacgatt tggaaaaaat ttataagctt
    11161 ctc
//
LOCUS       DAJOTB010000147        11046 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJOTB010000147 DAJOTB010000000
VERSION     DAJOTB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.147"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1819)
                     /gene="selB"
                     /locus_tag="OHN28_003921"
     CDS             complement(<1..1819)
                     /gene="selB"
                     /locus_tag="OHN28_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCQ2299225.1"
                     /translation="MIITTAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1816..3207)
                     /gene="selA"
                     /locus_tag="OHN28_003922"
     CDS             complement(1816..3207)
                     /gene="selA"
                     /locus_tag="OHN28_003922"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCQ2299226.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3305..3913)
                     /locus_tag="OHN28_003923"
     CDS             complement(3305..3913)
                     /locus_tag="OHN28_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCQ2299227.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3984..5120)
                     /locus_tag="OHN28_003924"
     CDS             complement(3984..5120)
                     /locus_tag="OHN28_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2299228.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLEPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5123..5485)
                     /locus_tag="OHN28_003925"
     CDS             complement(5123..5485)
                     /locus_tag="OHN28_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCQ2299229.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6022..7935
                     /gene="mtlA"
                     /locus_tag="OHN28_003926"
     CDS             6022..7935
                     /gene="mtlA"
                     /locus_tag="OHN28_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCQ2299230.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8255..9403
                     /gene="mtlD"
                     /locus_tag="OHN28_003927"
     CDS             8255..9403
                     /gene="mtlD"
                     /locus_tag="OHN28_003927"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2299231.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9403..9990
                     /gene="mtlR"
                     /locus_tag="OHN28_003928"
     CDS             9403..9990
                     /gene="mtlR"
                     /locus_tag="OHN28_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCQ2299232.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10001..10210)
                     /locus_tag="OHN28_003929"
     CDS             complement(10001..10210)
                     /locus_tag="OHN28_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299233.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10494..10856
                     /locus_tag="OHN28_003930"
     CDS             10494..10856
                     /locus_tag="OHN28_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCQ2299234.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2647 a         2989 c         2829 g         2581 t
ORIGIN      
        1 cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc aatgcggtca aaatattcca
       61 gaatctgaat tgccagcttg cggcctacgc ctaagcgatc gcggaaatcc gccgcgcagg
      121 ttgaaccaca ttcctgatcg agatcgcgga tcatattggc aaattcgaca atccgatcgt
      181 tacggtaata acgatctttg acgatcgcag taattatccc ctgttgggcc gcctggcgta
      241 gcgtcaggcg cattgcctgc tcgtcggttc ccgtctcttt tgccaggtca cgcacccacc
      301 atggctcgtc accaaacagc ggctctgctt tttgccagat ggcctgctgc tcttcgctga
      361 agcccgcttt atgatccggc agatgcagcc agccgtgatg gctgtggatg tcgccgcttt
      421 cgcgcatctt ttcaatcagc aacagtacca gcgcttcatc ttccattggc aacgccatac
      481 gtcgcagacg ttcgcgccca ggaccaggtt catcgcgatg ttgctcatga taagtcgcta
      541 atgtgtcgag aattttccgc tgccagcggg cggcaactgg cgcattcaac aagctgtaac
      601 cagcctgaat atagccaggc tgttgcagca gttcgcgcat cccttcgccg ttgagctgcc
      661 gtgcccaggc gaaatccgca aggttaaccg cgccgcgttc cagatgaaca gataacgcat
      721 cggcatcgct ctgcgcccgt gcaagagacg ccagccattg cagatattcc ggcttacgct
      781 taccgcgacg cggcgggtta agcatcacca cacgcgcccc cgccagcgtg ttgcgggccg
      841 agatatcgcg cagcactatg cgatcgttat ccgccagcca cagcggcgtg tcgaacacca
      901 gctcggcaag gttatcttcc agtagcgaaa cacgtcccgt aacgtggctg gcggcgtggt
      961 gaatatgcag cggctgccac tgggtcagcg gtgtatgggt ttgaagctcg acaatcaccc
     1021 gtgtgaacgg ctccggcggc acatcggcaa gcagccagtc gccacggtta atctgctctt
     1081 tttccgcatc acccgcgatg ttaagcgcga tacgctgccc ggcatgggca gtttctgttg
     1141 gctgattttg cgcatgcagc gcacgcacgc gcatcggttt atttacacca gtcagccaga
     1201 gtgaatcgcc tactttcact tccccgctta acgccgtacc ggtgacgacc agcccggcac
     1261 cttttacggt aaatgcgcgg tcgatcgcga ggcggaaact atgttggctg gcgtgttcac
     1321 gttctggtaa ctgaagcaga tgctcgcgca gggcatcaat tccccgacct tcggttgctg
     1381 cggtgataaa cagttttgcc tcagcaaaac cgtattcccg cagcacctcc tttacctggc
     1441 gttcaacctc atcaacacgc gcttcgtcca cgcgatcggc tttggtcagc gccactgtca
     1501 gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc acgggtctgt gccatcacac
     1561 cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc accaacgccc gccagcatgt
     1621 tggaaagaaa cttttcatga ccgggtacgt cgataaaacc aggcacgcga ccatccggct
     1681 gcggccagta ggcatagccg agatcgatgg tcatgccgcg ctttttttct tccggcagac
     1741 ggtcggcatt tacgcccgta atcgcctgca acaaggttgt tttgccgtgg tcaacgtgtc
     1801 cggcagtcgt aataatcatt tcaacaacat ctccaaaaac cgttgctcat cttcaaggca
     1861 gcgtaaatcc agccacaatc gtccgtcata aatacgacca atcaccggca ctggcaattc
     1921 acgccagcgg gcggctaatg actcaaggtg gctaccgcgt ccatcatggg gggtaaacgt
     1981 taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca ctgccgatct gcgaaagaca
     2041 tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg gcaaggagag cctgtaaacg
     2101 ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc aggcgcaggg tcggtaattt
     2161 ttcactcaga gcttcagggt gtaaataaag acgcaacgtg gcttccagcg ccgcgagggt
     2221 cattttatcc gcgcgtaatg cacgcttcag cgggtggctt tgcaggcggg cgatcatttc
     2281 ttttttacca acaataattc ctgcctgcgg cccgcctaac agcttgtcgc cggagaagct
     2341 caccagactg acgcccgccg caatcaactc ctgcggcatt ggctctttcg gcaaaccgta
     2401 ctggctaaga tcgaccagcg agccactgcc taaatcagtc accactggaa catccagttc
     2461 tttgccgagc gccaccagtt ccgcttcatc tatcgctttg gtgaacccct gaatgctgta
     2521 gttactggta tgtactttca tcaacagtgc ggtattttca ttcaccgcct gacgataatc
     2581 attcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg gtgcatcctg cctgacgcat
     2641 aacatcggga atacgaaacg cgccgccaat ctccaccagt tcgccgcgag aaaccaccac
     2701 ctctttcccg ctggcagtgg ccgccaacat caataacacc gccgccgcat tgttattaac
     2761 gatacaggca tcttccgccc ccgtaatacg gcacagcagc tgcgccagcg cccgatcgcg
     2821 atgtccacgt ccggcgtcgt ccagatcgta ctcgagggtc actggcgaac gcatagcctt
     2881 cgtaacggct tccaccgcgg cttccgcctg taaagctcgc ccaaggttgg tatgcagcac
     2941 ggttcccgtc aggttgatca ccggacgcag cgcgctttgc gcctctttcg tcaaccgggc
     3001 atcgacttct tgcgcccagt tttcacacca cgcgggcagc gtctggctgt cacgaatcac
     3061 ttctcgcgct tcgtcaagca tctgacgcag caattccacc acgcgggtgt gaccataagt
     3121 atcacgcaaa gaaaggaagg agctatcgcg caataagcga tcaatagccg gaagttgact
     3181 atagagggaa cgcgtttcgg ttgtcatagg aaacctggct gatcaaggcc ctctcacacg
     3241 gagaagggcg tttaacataa ccacggattg taacgtgaga tgggtcagga ggacatatcg
     3301 cgcatcaagc ctttggcggt tcggtgcgag caaaactttc gcggctaaac aggttttcga
     3361 ccagtttgac taagtgtggg cggtcgacac accagcctgg cgcaacgcgg cggaaattga
     3421 gatatccgac cgcacaggca atggcgatag tcgccagatt gaccgtatcg gttttgagcg
     3481 tgccatcgac cagatatcct tccagcacat ccagactgcg gttgattttc tcacgctgac
     3541 gtaacaattc atcttcagac tgctgcgccg ccggacgcgc ctgttcacgc accgatacca
     3601 gcccggcatc cataatgcca tccgccagcg cctcaatttt gcgcacccgc aacgactcca
     3661 gcggatcacg cggcaacatc gccggagcaa cattcattaa ttcaatatat tcagcgatga
     3721 tcggcgaatc aaaccagcat tcgccctctt cggtcaccag caccggcact tttcctaacg
     3781 ggttaaattg cgccacgccg ttgtccgcgt tatagggcag ttcattaata aattcgaacg
     3841 ttatgccctt ttctaacaac agaatagaaa gtttgcgtac aaacgggctg gtgtagctac
     3901 cgacgagttt catgccgagt cctttgtgcg aggaaaaata tcagtatggc tcaggctcgc
     3961 gcaaaacaca ggtgatacaa gtctcaatga tccaaataaa gataatgcat ccaactggtc
     4021 attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga gtaaactgcc
     4081 acctgggcgt agatgccgtc gggtaaggca tcgatatcag cgttagggtc cagctcaatg
     4141 gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac cccctgcgcc
     4201 tgataagaac cgcctggcac gacaggtaaa atactggtca gtttgccgtg aaacacctgc
     4261 ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg taacagcgag
     4321 ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac catcaccgga
     4381 cgcagcggca aggcagcggc gtatgtacct gggcggatca gtacctgagt gacgtagcca
     4441 ttgctcggtg cgcgaatgac ggtctgctca aggttatatt ttgcttcagt aagttgcgct
     4501 cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg gctctggatc
     4561 tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa attttgccgc
     4621 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcagataa
     4681 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     4741 gcttcagtga gttgcgcgcg cagcgtcttt atattatgcg tcgccgtcat caggtctgcc
     4801 tgaagtctgt caactcgcgc ctggtaacga accggctcga gcttaaaaag cacctcgccc
     4861 ttctgaataa gctgattatt tttgtcggtg acttcagtaa caattcccgt cacctgtggc
     4921 gtgatgggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     4981 atcaacaaga tcaaaccact caccagaaac acgcctccca gcgccgccgt cgccagcgtc
     5041 cactgattta ccggaatgcg gaagatttta aagaccgccc acgccagcgc cacgtaagtt
     5101 aaaacaatca atagatccat atttagatct ccggaaacgt ggaatagtca gtctttttct
     5161 cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg atttccgggt
     5221 cagtcgcttt ctcttgcgac gcaacatgtg actgcatgcc ccagccgcgc tccggttgat
     5281 agagcgtcgc ccagatccac aggaacggcc agataacatg tagagtaaac aggctcaccc
     5341 agcccgccgt atgaatagcg tcggcatggg gatggttgcg ctttttggca atcagataag
     5401 ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag atcagcactc
     5461 ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca gtgttgtttt
     5521 ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt
     5581 ctcgccataa caccaaagaa taattttaag aggtgatgag ttgcttagtt acataacgat
     5641 ttcatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc
     5701 ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cattactcac
     5761 atttaaatgc catttttagc gaaaatcgcc gccttgttgc ttttttacac aagcattttg
     5821 tgatgagcat cacgtcaatt acctctctac cccctacatt tatgtgactg atatcacaca
     5881 aaaggccgcc gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt
     5941 ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca
     6001 aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt
     6061 cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc
     6121 accgcgttat ttattccaac agggtggtta ccgaacgaaa cgctggcgaa gctggtcggg
     6181 ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa gctggttggc
     6241 ggcgaacgtg gcggcgtagt cggtgccatt accaccatgg gcgttatcgt cggcgcagac
     6301 atgccgatgt tcctcggctc tatgattgca ggtccgctgg gcggctggtg cattaagcac
     6361 ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc
     6421 tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat tggcccgatt
     6481 gttgaagccc tgtccaaaat gctggcggcg ggcgttaact tcatggttgt ccatgacatg
     6541 ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt
     6601 aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatccatc
     6661 ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc gtacatgttc
     6721 tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca cttcctgggg
     6781 ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat tctggccgtt
     6841 atcctcggcg gtatgactgg cgtattcacg ctgactatcc tgggcggtgg gctggtttct
     6901 ccggcatctc cgggttctat ccttgctgta ctggcgatga cgccaaaagg tgcttacttc
     6961 gctaacatcg cgggtgtgtg tgcggcgatg gcagtttcct tcgttgtctc tgccattctg
     7021 ctgaaaacca gcaaagtgaa agaagaagat gatattgaag ctgctgcacg tcgcgttcag
     7081 gaaatgaaag ctgagtctaa aggtgcgtct ccgctgtctg caggcgatgt gactaacgac
     7141 ctgagccacg tccgtaaaat catcgttgcc tgtgatgccg gtatgggttc cagtgcgatg
     7201 ggtgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcgcagat ttcggtcact
     7261 aacagcgcga tcaacaacct gccgccagat gtggacctgg tcatcactca ccgtgacctg
     7321 accgaacgcg ctatgcgcca ggttccgcag gcacagcata tttcgctgac caacttcctc
     7381 gacagcggcc tgtacaccag cctgaccgaa cgtctggttg ctgcccagcg ccatactgaa
     7441 aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag tgctaacctg
     7501 ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cggccaccaa agaagaagcg
     7561 attcgttttg ctggcgagca actggtgaaa ggcggttacg ttgagccgga atacgttcag
     7621 gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat cgcggtgcca
     7681 cacggtacgg ttgaagcgaa agatcgcgta ctcaaaacgg gcgtcgtgtt ctgccagtac
     7741 ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt gattgggatt
     7801 gctgcccgta acaacgagca cattcaggtg atcaccagcc tgaccaatgc actggatgat
     7861 gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct ggaattgctg
     7921 gcaggtcgta agtaatccat tcccaccctc tccgcatgaa gaaggtgggg ctaattgctt
     7981 gatgcgcttc gcttatcagg cctacaacat aaagcacaat ttgttttgtc ggtcggataa
     8041 ggtgtttacg ccgcatccgg cagtcggtgc acaatgcctg atgcgacgct gacgcgtctt
     8101 atcagaccta caggtcagtg ccaaacacgt aggccggata aggcggttta cgccgcatcc
     8161 ggcaaaaagc ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc
     8221 ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg
     8281 taatatcggt cgtggcttta tcggtaaact gctggcagac gcaggtatcc aactgacgtt
     8341 tgccgatgta aatcaggtgg tgcttgatgc cctgaatgcc cgtcatagct atcaggttca
     8401 tgtggttggt gaaaccgagc aggtagatac cgtttctggc gtcgatgccg tcagcagcat
     8461 tggtgatgat gtcgttgatc tgattgctca ggttgattta gtcactaccg ccgttggccc
     8521 ggtcgtgctg gaacgtattg ctccggccat cgccaaaggg ctggtgaaac gtaaagaaca
     8581 aggtaatgaa tctccgctga acatcatcgc ctgtgaaaac atggtccgcg gcaccacgca
     8641 gctgaaaggc catgtgatga acgccctgcc cgaagacgcc aaagcgtggg tagaagaaca
     8701 cgttggcttt gttgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa
     8761 cgatccgctg gaagtgacgg tagaaacctt cagcgaatgg attgtcgata aaacccagtt
     8821 caaaggcacg ctgccaaaca tccctggcat ggaattaacc gacaatctga tggcatttgt
     8881 cgaacgtaaa ctcttcaccc tgaatacagg tcatgctata accgcgtacc tcggaaaact
     8941 ggcgggtcat cagaccattc gtgacgcaat tctcgacgag aaaatccgcg cggtggtaaa
     9001 aggggcaatg gaagaaagtg gtgcagtatt gatcaagcgc tacggctttg acgctgacaa
     9061 gcatgcggca tacatccaga aaatccttgg tcgtttcgag aacccgtacc tgaaagatga
     9121 tgtagaacgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa
     9181 gccactgctc ggaaccctgg aatatggtct gccacataaa aacctgattg aaggtattgc
     9241 cgctgcaatg cacttccgca gtgaagatga tccgcaggct caggaactgg cagcgctgat
     9301 tgccgacaaa ggcccacaag ccgcgctggc acagatttcc ggtcttgatg ccaacagcga
     9361 ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag
     9421 gataccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag
     9481 gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg
     9541 atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc
     9601 aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga tggtccgctg
     9661 ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa
     9721 tacgaagatg cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag
     9781 tacgctttta ccgacgacga aatccttgga ccctttggtg aactgcattg cgtggcggcg
     9841 ttaccaccgc cgccacagtt tgaacctgca gactccagtt tgtatgcaat gcaaattcag
     9901 cgttaccaac aggctgtacg atcaacaatg gtcctttcac tgacagagct gatttccaaa
     9961 atcagcttaa aaaaagcctt tcaaaagtaa gtactttcgc ttacttctcc tctacctgtt
    10021 tcggacgata aagctgacga taatactcca gacgttgtag atataacggc acgctttcat
    10081 caggtattcc ggacggaatc gcgttacggg gaggtagttt tttcagatac tcccggaacg
    10141 cctggctcga tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta
    10201 atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt
    10261 agtttagatc ccggtataaa tgacattaag gaaaacgtgc tcaatcctca ataatcacct
    10321 gcttctgttg actatctttt ttctttaaat ttcttaacta atcaatgtat tgggtgattt
    10381 tttaacgcat taatggttac aatctggaac tattacaatg ccatcagttg tgaacaaagc
    10441 acctggtcgc gcatactaga ggctataaat ttatctctat cagaagtcat cacatgaaag
    10501 aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct ggacgccatc cgtcaccagc
    10561 aggccgatct atcgctggtt gaagccgcag acaaatatgc cgaactggaa aaagagaaag
    10621 ccacgctgga agcagaaatt gctcgcctgc gcgaagttca cagccagaaa ctcagcaaag
    10681 aagcgcagaa actgatgaag atgccgttcc agcgtgcaat caccaaaaaa gagcaggctg
    10741 atatgggcaa actgaaaaaa agtgttcgcg gactggtggt tgtgcaccca atgaccgcgc
    10801 tgggccgtga aatgggcctg caagagatga cagggttctc aaagactgcg ttttaagttc
    10861 ctttctcgtc gctgtaacta ctgccactca cgatttactc gctcctgctg gcacatgcag
    10921 gagcctgtgc cactcattaa atacgatgtc tttcttaccg taaactgttt cagtttcccc
    10981 tcgagtttga ggtatttttg caggctaaaa tctgaaaatt tgttgcgcaa caacaagtga
    11041 tatata
//
LOCUS       DAJOTB010000148        11036 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJOTB010000148 DAJOTB010000000
VERSION     DAJOTB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.148"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..841
                     /locus_tag="OHN28_003931"
                     /pseudo
     CDS             1..841
                     /locus_tag="OHN28_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(976..2241)
                     /locus_tag="OHN28_003932"
     CDS             complement(976..2241)
                     /locus_tag="OHN28_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCQ2299235.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMLLLVHPNGSRYWRLRYRI
                     FGKEKTLALGVYPEVSLSEARAKRDEARKLISAGIDPCEQKRAKKVAPGLQLSFEHIA
                     RRWHASNKQWTQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQSVTRLAVMLNLLVFIRSSELRYARWSEIDIENAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLVFTGAHDPHKPISENTVNKALRVMGYDT
                     TQDICGHGFRTMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLGERRLML
                     QWWADFLNANRERFISPFEYAKINNPLKQ"
     gene            complement(2705..3031)
                     /locus_tag="OHN28_003933"
     CDS             complement(2705..3031)
                     /locus_tag="OHN28_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2299236.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(3028..3291)
                     /locus_tag="OHN28_003934"
     CDS             complement(3028..3291)
                     /locus_tag="OHN28_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HCQ2299237.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVAAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(3363..4229)
                     /locus_tag="OHN28_003935"
     CDS             complement(3363..4229)
                     /locus_tag="OHN28_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2299238.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            complement(4314..4511)
                     /locus_tag="OHN28_003936"
     CDS             complement(4314..4511)
                     /locus_tag="OHN28_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299239.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(4523..5011)
                     /locus_tag="OHN28_003937"
     CDS             complement(4523..5011)
                     /locus_tag="OHN28_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299240.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(5008..5385)
                     /locus_tag="OHN28_003938"
     CDS             complement(5008..5385)
                     /locus_tag="OHN28_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2299241.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(5432..5806)
                     /locus_tag="OHN28_003939"
     CDS             complement(5432..5806)
                     /locus_tag="OHN28_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2299242.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(5886..6107)
                     /locus_tag="OHN28_003940"
     CDS             complement(5886..6107)
                     /locus_tag="OHN28_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2299243.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6170..6646)
                     /locus_tag="OHN28_003941"
     CDS             complement(6170..6646)
                     /locus_tag="OHN28_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2299244.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(6662..7141)
                     /locus_tag="OHN28_003942"
     CDS             complement(6662..7141)
                     /locus_tag="OHN28_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2299245.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMVPEPDDDDDETWVLFNVMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(7223..8041)
                     /locus_tag="OHN28_003943"
     CDS             complement(7223..8041)
                     /locus_tag="OHN28_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2299246.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(8131..8364)
                     /locus_tag="OHN28_003944"
     CDS             complement(8131..8364)
                     /locus_tag="OHN28_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2299247.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(8370..9047)
                     /locus_tag="OHN28_003945"
     CDS             complement(8370..9047)
                     /locus_tag="OHN28_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299248.1"
                     /translation="MNTRMLFPLLFTVASFSTSAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEVGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTPAINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            complement(9166..10050)
                     /locus_tag="OHN28_003946"
     CDS             complement(9166..10050)
                     /locus_tag="OHN28_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2299249.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDMPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAVAQFSFPAWMLQLLR
                     KARARIIHLLVTLWDHLF"
     gene            10235..>11036
                     /locus_tag="OHN28_003947"
     CDS             10235..>11036
                     /locus_tag="OHN28_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299250.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLTELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDALSELYAIYSWVSYCKRGDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVPGHNDSKSLYKTVLSVC
                     GGKRETEMFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGG"
BASE COUNT         2747 a         2615 c         2854 g         2820 t
ORIGIN      
        1 gaaatcgttg ccacgttcaa agaaatgtac gacgccgatg tctcgcccac gctgatatcc
       61 aaagtgaccg atgcggttaa agaacaggtc tctgaatggc aaaatcgacc gttggatgca
      121 ctgtatccca ttgtttatct tgactgtatt gtggttaagg ttcgtcacgg cgggagtgtc
      181 attaacaaag cggtgttcct cgcgctgggc atcaataccg acggtcagaa agagctgctg
      241 ggtatgtggc tggcagaaaa tgaaggtgca aagttctggc tgggtgtact gactgagctg
      301 aaaaatcgtg gccttcagga catcctgatt gcctgcgtgg acggcctgaa aggcttcccg
      361 gaagcgataa atagcgtgta cccgcagacg catattcagc tctgcatcat ccatatggtg
      421 cgcaacagcc tgaaattcgt gtcgtggaag gattacaaag ccgtcaccgg tatatcaggc
      481 cccgacagag gaagcggcgt tgatggcgtt ggataagttt gcaggcgtct gggatgaaaa
      541 atacccgcaa atcagcaaga gctggcgcac acactgggaa aatctcaaca ccttcttcgg
      601 ttacccaccg gatatccgca aggccatcta cacgacgaat gccatcgaat cgctgaacag
      661 cgtcattcgg caggcgataa agaagcgcaa ggtgttccca acggacgact cggtgcgaaa
      721 agtgatttat ctggcgatac agtcggcgtc aaaaaaatgg agtatgccaa tccagaactg
      781 gcggctggca atgagccgtt ttattatcga gtttggtgac cgccttagcg agcaccttta
      841 atacggcggc agttacacag aattatttac agggtctgca atcaggtaaa cagtaaatac
      901 atcagttcat accgtttgtc ttttttaaca aagaaaatag tcctggagta gttcccgggc
      961 atttgcccgg gatggttact gttttaacgg attgttaatc tttgcatatt caaatggact
     1021 gataaatctt tctctgtttg cattcagaaa atcggcccac cactgtagca tcagtcggcg
     1081 ttctcccaga tgttctgctt tatgaatgta agcagcacgt acaccattac gctcctgatg
     1141 actcatctga cgttccactg catcacggga ccacaatcct gattctatta acgcgctaca
     1201 tgccatcgtc ctgaatccat gcccacagat atcctgggtt gtgtcatacc ccataactct
     1261 gagggcttta tttacggtgt tttcactgat tggtttatgc gggtcatgtg ctcccgtaaa
     1321 gaccagacca ttttcaccag cccatgtctg aagttctgta agaatagcta cagcctgctt
     1381 gctgagggga acaagatgcg gagttctcat cttggaaccc cggtgcgaga attttacgcc
     1441 tggcagaggc tcgcgttcag caggaatagt ccacatggca ttttcaatat caatttctga
     1501 ccagcgggca tatctgagtt cactggagcg aataaaaacc agtaaattca gcataacagc
     1561 aagtcgggta acagactgac ctttataact gccaatacgg gaaagaagtt caggaagacg
     1621 tgaaagatca agagcggggc gatgctggcg ttttacagtg gtcaatgcgc cagccatatc
     1681 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cagcagatat
     1741 cctttgctgc agacgactgg cgatttcata aatctgttta gcctctgcag cgcgaactgg
     1801 gataagcagg tccggagtgt tgagtgttgt gatatcccgg ttgccgataa aagggaaaac
     1861 atgcgtctcg aggcttttga gtactttatc gctgtgtgat tgtgtccatt gtttattact
     1921 ggcatgccag cgtcgtgcaa tatgttcaaa agagagttgt aaaccagggg ctactttttt
     1981 agctcttttc tgttcgcaag ggtcaatccc cgccgaaatt agttttcggg cttcatcccg
     2041 ttttgcacga gcttcggaga gagaaacttc tggatacaca ccaagtgcaa gagtcttctc
     2101 cttaccaaaa atacgataac ggagacgcca gtaacgggaa ccattgggat gtaccagcag
     2161 gagcatgcca gctccgtcag tgagtttata cgccttgtca gtgggctttg cagcccgaat
     2221 ttttgcgtca gtcagtgcca tatttcagcc ttctgaatgc gttaaagagg gtacaaattc
     2281 tgtcgaactg aggtataccc gcaattatac ccacacaatg gggttgatgt ggggggaatc
     2341 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     2401 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatgaa
     2461 actggttgta taacaagttg ttttatattg attgttttgt gtttgttatt tttgaggcca
     2521 ccaatgaggc ccccatgtgc aattcgaatt cttttgacgc tataatccct ctttcttgtg
     2581 gtttgttgta atcattttgt ctggttcgat atgctgaatc gcggaatcga agtgacaaca
     2641 aaatacagag atttataaaa tacctggcta cctgtgttag ggagcgttaa cgcacagatt
     2701 ttgtttatgg ctgccagaaa tgatctgatg gtttgtcgtc tggctctgca agtaccctgt
     2761 tgatatcccg ggaaccatga acgatacgtt cgatacgaac ttcagtatcc agtaccctga
     2821 acagcagaag gtaacgacca tacgaacaac ctctgatccc agcccctaac tcgggtcttt
     2881 ctctgtagag tactggcgat tcggctatca acagacattg ttggtaaagc tcttcggtaa
     2941 aactgattgc acggacagga ttatcctgag cgatccagtc acctatagct tcaagatcgt
     3001 gttctgctaa tggtgaaaca gtcagtttca tattggctgt tcaccttctg ccatcttttt
     3061 gtatttgtgc gtcagtcgcc cgaatacctc ttcagcactt ttactttcac cgctgttgat
     3121 gcctgcggcc acggcgctct ggagtgattc aagctttatc tgctgttccc gttcttccag
     3181 agcacgaagt ccggcacgaa tgacttcact tgtattgttg taccgaccac tttctatttg
     3241 ttcgcggata aacgtttcaa agtaaggact gagggataca ctggtaggca tggttatttt
     3301 ctcgccgatt gattactatc aattaataat agttaatatt cgcgaattgt aaaatacctc
     3361 ttttatgcct tgcggctttt gttcactgtg cttgccaggg cttccgagta gtgtctggca
     3421 ataatatcat taagcctgtc aaccagttct ggtttcctga acgtgatgtg cgcggagccc
     3481 ttcttaaagt atctgatgct gaacatctca tcttcatagt cctctttacc ctgaacagac
     3541 tggatgtggt catccagatg aatggtgatg ttttcccggt tgtcggggat cggtttgccg
     3601 ctgaacagat acagcattct ttccaggtcg gcaagtcgat ctgcctgttg cccggtgata
     3661 agatgaaacc cccaccggtc ccacctcacc aggttgttga cgataatttt actgccaaat
     3721 ttacagggtg aattgttttc gtaattccag ctcagtcccc tgaaaacatt gatcacccca
     3781 cgctcaaaca cttcatcttt gttctggtgc agttgttcaa atgtgctcag aatgttcgct
     3841 tcactgattt ccggaaaatt atcgtattcc agtgaccggt accatgtatc acgggcctgc
     3901 gcatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat ttcgcgatcg
     3961 agattgcggg tgatggcttt cattgctgtt tccggtttat ccatcagcca gcagccacag
     4021 cgaaaatcct gtttcatggc ccagtcccgg gcggttttac ctccaatgct ttccgtcaga
     4081 actgagattt cagtcagttg ccggataagt gtgtcgattt gcagcagtgt actctttcgt
     4141 acagccagaa tgtggcggat actggttgag cagataacgt ccgtatgccc gttgaccact
     4201 tccggacggg aggctgtgat atcagacata atcgatattc ctttttgcag ataaatgaaa
     4261 acgccagccc tgttcaggct ggcgtcagtg gaatgaagtc tggtgagtaa tatttaccgg
     4321 ttctggtgca gaagctgcag atgacgaaga gtacggacat cctcccgggc ccgttcgata
     4381 cagggcaaca gtgctgccga atccgtttta tcctcatcgt tgtcaaagat aattcccgat
     4441 tcgcagtcga tattgtcctg cagccacaca atcagaatat ccagtgctgt ttccgtggtt
     4501 aatgatttca tgttgttcct ggttaccagt cgaaagtggg caggtcaggc aggcattcag
     4561 cgctggcatc cagatgaatg agactgacgc cataacgccg gataagagtg acaaccagac
     4621 ggcggaacgt ttttgacagt cccaggcgtt taagtcgcag aaccgggtat gaccacgcat
     4681 cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattcg ccggtgtcat
     4741 tcgtctgatg tgacagcgag tacaaccagg cattgtccgt ttccgtgata tgtgcggtac
     4801 tgcagcgtat gccggtaagg gccgcagatg gtggcgtgcg ctctgctgat gcccggtcag
     4861 attcatcaac aacacgaagg gagtaaccgt tatcgcagac cacgttaatc agttcagcga
     4921 gagtaacacc atcgacgtca acgacaatgc gccccatgtt cagtgcctgc acgttaatgc
     4981 tgtcggcttc cagcgtcagg gccagtttca ttgtttcgcc cccggatgtt tacccaaggt
     5041 aatgttattt accgttctgt aattgtcgcg ggccatcagg ccggttgccc ggcgggcccg
     5101 gaggatatca atactgttta ttaactgaga acgggtacag gcgctgaatc ccggctggtc
     5161 ggtacgtacc agtgcgtatt tttcaacgag aaagttcacc gcatcgcaca gtgaaatgcc
     5221 tgcctcaata tgctgctcaa tcacacgttc atcagcgaac ggtgtgtcat tcagtgtgag
     5281 gccgtaatgc tggtccaaca gtcgggtgag cagtgtctgc cagatggtga cgggagacgg
     5341 gcagcgcgat gcctcccgta cgtgtgtgtc aggtaatgtt ttcatcttaa gttctctgta
     5401 actggtagtg ctaaaggtga gatattcggg gttatgaggt ggttgcgggt gccagtgtcg
     5461 gataaacagc catataaacg taaccacagc tgccgagggt gtcggcttcg caggtcagcc
     5521 ctctggcata cagcgtgacg gtatgctgat ggcggggatt cagttcaccg ctggtgagca
     5581 tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatgatac gcatccgcgt
     5641 cgctaaactg ccccctgata ccggcgcggt cggcaaggta atgcaactga ttgccctcct
     5701 gcaccagacg tgccccgaaa cagggcgtca cggtgcaggg gagcccccac caggggcggt
     5761 cgttgttgtt gtcgggatgc gttgtcccgg ggtgtgtgtc tgacacgata aaatcctcac
     5821 agaaaatcgg ttaagaatgc tccgatggtg gcgataattc ggctcatcag aatgcccttt
     5881 ctgattcagt tcagggtaac gctcatcaga cggacatacg gaccaaaact gtccttacgg
     5941 cgttcagcaa acacggccag cacaccggga atatcctgca cttcacggcc ggtatacgta
     6001 tccgtgctac cgtgccagcg gtatttaccg gtacagaacg gaaaaagacg ggatgcagga
     6061 tgctgttggt gaatacgcat ggcttcacca cgggtgatga ttttcataat gggatacctc
     6121 tgaagacaga agataaaagt gaaaacaggt gtgatgtggt tgtgacgggt taaagcagac
     6181 cgtgttcggc aaaggagaat acctggttgc caccgactat cagatggtcc ggcacccgga
     6241 tatccaccag ggccagcgcc tgtaccagac gttcagtgat aagccggtct gccttactgg
     6301 gggtgacttc accggacggg tgattgtgcg ccagcaccac ggcagcggca ttgtggtaca
     6361 gggcacgttt aatcacctcc cggggatgga cttccgtgcg gttgatggtg cctgtgaaga
     6421 gggtttcacc cgcaatcagc tgattctggt tgttcagata cagcacccgg aactcttccc
     6481 gctccagtcc tgccatgttc agaatcagcc attcccgtgc cgcatgcgtg gaggtgaagg
     6541 ccacgccggg ctcatgaaga tgacggtcca gggttttcag ggcccgctga atgaggctgc
     6601 gctcgccggg cgtcatcgct ccgggcagaa aggaaagctg ttgcattatg cttctctcca
     6661 ttcagtcgat gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca
     6721 accgataata atggaccgtc atggcataat tctccgtacg acaggcgtga tgactgtacg
     6781 tcatcagaca ggcggcaatg ccggcggctt ccgggctcat ttcagcgcgg ttaccgttca
     6841 tgacgttaaa cagcacccat gtttcgtcat catcgtcatc cggttcgggt accataaatg
     6901 ccccaccgtt gttcagggtg tacagattcc agataccacc gcagtagtct tcgcaaagac
     6961 ggtccatcca gccgaagaca cggggctcca gggtcaccca ctgtggaatg aggccaaaat
     7021 gctgcggcca aaagctgatg cgctgttcat cagggacgag gctggcaacc aactgaggcg
     7081 gattattctc tggtgttgta gctgaataaa ttgtgggggt attctgagaa acgcttttca
     7141 tgagataact ccttaaataa caaataaaag tgtgtttttc aggaacgaaa gctgaatgat
     7201 tccctcactc agggaaacgg catcagcgca ggctctccag catcgtttct gccatcaccc
     7261 acaatgcgcg gttgagttta atatcggtgt cgatgctgtg aatggcccgg gtatggatac
     7321 gttttccttt tgcactgcga ccggaaatcc cgcctttcag catattctcc tggatggtct
     7381 gatatgcgct ccacaggtcc ttaccgtaat cctcccggcg tcgcggcgtc agaatgtcgg
     7441 cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc tgtgccagcg
     7501 cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttc tcctctatcc
     7561 ggtcaaacac gcccaccact tcgtaagccc cttcaatgac tctgtccacc acatttcccc
     7621 ggtgcggaac acgcacttcc cccagagact ggccacagac gcacccgttc tggcagacga
     7681 acctgaagta acccggcagc atctggtagc tggaggtacc gtcatgagag ttgagcagaa
     7741 taatttcagg gacatgttct ccgtttatct ctccggcccg ccgcagacgc agcatgtgtt
     7801 tggtatattc ccggcggctc tggtcgcgca cacgggtctg gcaggcgaag aacggctgaa
     7861 agccttcccg ctgcagattt tccaggacgg taatggtggg aatgtacgca taccgttcac
     7921 tgcgggaggt gtgccggtct tctccaaaaa tactcgggac gtggtacatc agttcttcgc
     7981 gtgtcagtgg gcgctcccga cggatctgat ttacatgacc aaaacgactg gctaattgca
     8041 tactttgctc cttatgaatg aattagcggt ataaacgaaa aagccgcgtc tcccggagaa
     8101 gacgcggcct gatggatgaa atgaagacgt ttattgcctg agaattccct cactggcgag
     8161 atacgaattt agctgttttc cggcatcagg ttcaaaattc cagcaccgcc agcgtagctg
     8221 cccttcggca ttgcggataa ccagccggaa atgcgttccc tggtcatctt caataagcac
     8281 attgcggtaa gctgttgtga cggcaaccgc ctgttcgcgt gagaaagggc cttcgggcaa
     8341 taaaacaggg tggcagtccg acataccgac tattttccga taaattcaac gctggcgctg
     8401 gtggcattac cgtcagcacc aaccgtgacg gtcactttgt cgtacgcacg gtcttcagga
     8461 ttgaatactt caaactcaac cttattgcct tttacttcgt aagagccaaa atcagcggtt
     8521 tctgttgttt cctcgtcata ttcccgggcg tatgcctgcc gggcagcctg caggacggaa
     8581 tctttattaa tggcgggtgt ctgtttcggg gaaaaaccgg caagtgatgt gacaaatttc
     8641 ggacagacct tcagcatatc accgcgggtg aattccattc gtacaccaaa tgcctgtaaa
     8701 acggacacat cacgctctgc tatggagtga acggcttttt tgaccatttc cggcgcctgt
     8761 ccgacttcca cctcaacagc cctgaatcct gtctgctgat tacacatttg tgatgtgggg
     8821 atcaggttac cgttaatact gatacggctg cccaggagcg tctgtacgcc attcgggcat
     8881 ccctgcatca ggccggtaat accaatcgtg ccatttttca gcattaccag cgcctgacca
     8941 tcttccgtca tggtctgaca ccagccgttt ttgacagccc agttaccggc ggaggtggag
     9001 aaggatgcca cagtgaacag caggggaaaa agcattctgg tattcatgac tgacctctga
     9061 aattaaagag cgtgttattg ataatgacag acggtatacg gcagtggtta ccgtaatccg
     9121 ttgtcgtgta aaggtatacg gttgccacgg gggactccgg ggcgctcaga acaggtgatc
     9181 ccacagtgtg accagcaggt ggataatgcg ggcccgggct ttacgcagaa gctgtaacat
     9241 ccaggccgga aaactgaatt gggcaacggc agcgtcaaag cttttcccga tggcgtcacc
     9301 aaaagtttgt tgtgcgtgtt tccgggcctg ttcagagcgg ttttcccccc ggatatgtga
     9361 ataaactgcg ctggttgcct gtggtggcag tgcgtggatc atcagcgaca ccagtgccgg
     9421 aagattccag ccgacaggtg cggcaacgga gagtaccgga aatgatgacg ggaacagggt
     9481 tgccacccgg gctgttactg tcgccagtga gagttcctgc tgacgggacg ggcatttttc
     9541 tgtgtcgttc cattcttcag caggaaacac gcgatcggca tggctgagaa caaacagaaa
     9601 acgcgaggga tctgcacctt cattcagtaa aaactgatgc atggcaatat cagcagcata
     9661 cgcacgttca tcagcccgca ggatccagat aatcagatcc agttccggca gtaactgacg
     9721 gtaaagctcc cggtattcct gatcatgctg cggtgtctca ccaatgccgg gcatatcgac
     9781 cagcgtcatt ctgcgttcac cgagctgcag ggtaagacga tgagcctggc gggtgcagcc
     9841 gttcagggga tgcgtggcgc agatacggga ctgaaaaatg gcattacaca gactgctttt
     9901 gccggtgccg gatttaccca taatgccaat tacgggctcg taatgaataa caccgtggag
     9961 atgttcaagg attttctggc gaatttcttc aggaagaaac gtcagatatg attttacttg
    10021 tgcagagagg gaagaatggc tgaatgacat accggtatcc tgttgatttc gctgaaccgg
    10081 atcacactaa tccggtttat cattcaggta atatggacgt gaaaaatgat ggaatgtctg
    10141 aattacggac atgattgtta aagtgtaatg gagtatgaag tgagtttttc cgaacgggaa
    10201 ctaatgacat actgcggttt caggagatga gaggatgacc ggacgtaaaa acaaatgtgt
    10261 cagaaaaaat tgtaacaatc aggttttctc agtaaaaatg gcagtggaga aaagactgtg
    10321 cagaaaacat tatgagaggt tttgtgagct caaaaaaaga agagagcaaa atcctgttca
    10381 cagattaaat gaagcattgc caagaaagat aaaaggacca aaaagtaaga aaaaattagt
    10441 aaaagaaaga gaaatcatta acagcagaaa aaaacataaa caggagcagg gaccatcgct
    10501 gactgaactt aataaaaaag ttcgtgagat actgaaaact cctgcatgga gaacgctgga
    10561 aacgagaatt cgaagaaacg gaatcacatt taagtcaaaa gcagagttag atgcgctttc
    10621 tgaactttat gcaatttatt catgggtttc atattgcaaa cggggcgata attattctgt
    10681 gtggtttttg aattgtccgg atgtaaatat aaaccttcat gtgtgcgaat gccggtcttc
    10741 tgaaagcgat tgttatctgc catctcgttt tattattctt ccagctccat taaggcaaaa
    10801 agtaaaaaga aaatactcgg ctaattcgtc tggggtacca ggacataatg attcaaaaag
    10861 tctttataag acagttttgt ctgtttgtgg tgggaaaaga gaaaccgaaa tgttctttta
    10921 ttcattgcca gtaaagacta atcatcatcc ttactggatt aaaagtggtg tggttaaaag
    10981 attccctcct gtttttggtt tgttgtttta tgaacaggaa agattaacag gaggtg
//
LOCUS       DAJOTB010000149        10860 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJOTB010000149 DAJOTB010000000
VERSION     DAJOTB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.149"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            153..1886
                     /locus_tag="OHN28_003948"
     CDS             153..1886
                     /locus_tag="OHN28_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCQ2299251.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKAL
                     PEHGIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEARRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
     gene            1883..2797
                     /locus_tag="OHN28_003949"
     CDS             1883..2797
                     /locus_tag="OHN28_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCQ2299252.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2829..3077
                     /locus_tag="OHN28_003950"
     CDS             2829..3077
                     /locus_tag="OHN28_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2299253.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENN"
     gene            3077..4249
                     /locus_tag="OHN28_003951"
     CDS             3077..4249
                     /locus_tag="OHN28_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2299254.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISTLADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4284..5363)
                     /gene="lptG"
                     /locus_tag="OHN28_003952"
     CDS             complement(4284..5363)
                     /gene="lptG"
                     /locus_tag="OHN28_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2299255.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDNDIIGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNYKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5360..6430)
                     /gene="lptF"
                     /locus_tag="OHN28_003953"
     CDS             complement(5360..6430)
                     /gene="lptF"
                     /locus_tag="OHN28_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2299256.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFPR"
     gene            complement(6461..7021)
                     /locus_tag="OHN28_003954"
     CDS             complement(6461..7021)
                     /locus_tag="OHN28_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2299257.1"
                     /translation="MPSRWIVALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7021..7857)
                     /locus_tag="OHN28_003955"
     CDS             complement(7021..7857)
                     /locus_tag="OHN28_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299258.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFFGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMDNRRSYDAPVALDSDIRQRLELLISRFPLYP
                     EQ"
     gene            complement(7857..9206)
                     /locus_tag="OHN28_003956"
                     /pseudo
     CDS             complement(7857..9206)
                     /locus_tag="OHN28_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
     gene            complement(9252..10010)
                     /locus_tag="OHN28_003957"
     CDS             complement(9252..10010)
                     /locus_tag="OHN28_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299259.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISRF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(10042..10755)
                     /locus_tag="OHN28_003958"
     CDS             complement(10042..10755)
                     /locus_tag="OHN28_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2299260.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
BASE COUNT         2733 a         2833 c         2669 g         2625 t
ORIGIN      
        1 taaatatcat tcacaataaa aacaaaaaag ccaaacctat tatttaaatc aatgttcatt
       61 ttcacaccga tgaaaatact atctcattta tcttttatgc ctatataatc caccgcatta
      121 ttgtcatttt atttttgata tccaattttt tcgtggtgta tatgtctaat aaaatcttta
      181 cgcattccct acctatgcgc tatgccgatt ttccaacgct ggttgatgct ttggactacg
      241 ccgctctgag tagcgccgga atgaattttt atgacagacg ttgccaactt gaagatcaac
      301 tggaatatca gacgttaaaa gcgcgtgccg aagctggtgc gaaaaggttg ttatcgctga
      361 acctgaaaaa aggcgatcgc gtggcgctga ttgccgaaac gagtagcggg tttgtagagg
      421 ctttttttgc ctgccagtat gccggcttag tcgccgtccc gttggcgatt ccaatgggcg
      481 taggtcagcg ggattcctgg agcgccaaac tgcagggttt actggcaagt tgccagcccg
      541 cagccatcat cactggtgat gagtggttgc cactggtcaa tgccgcgacg cataacaaca
      601 accccgaatt acatgtttta agccacgcct ggtttaaggc attaccggaa gccgatgttg
      661 cgctccagcg tccagttcca aacgatatcg cctacctcca gtacacctcc ggcagcaccc
      721 gttttccccg tggcgtcatt atcacccatc acgaagtgat ggctaatcta cgtgctataa
      781 gccacgatgg gattaaatta cgccctggcg accgctgcgt ctcctggctg cctttctacc
      841 atgatatggg actggtcggc tttctcctga cccccgtcgc cacgcagctt tcagtagatt
      901 atttgcgcac tcaggatttt gccatgcgtc ctctgcaatg gcttaaattg atcagtaaaa
      961 atcgtggcac cgtttccgtt gcaccgccgt ttggctatga attgtgccag cgccgcgtga
     1021 atgaaaaaga tctcgctgaa ctggatcttt cctgctggcg cgtcgctggt attggcgcag
     1081 aacccatatc cgcagaacaa ctccatcaat tcgctgaatg tttccgtcag gttaactttg
     1141 acgataaaac tttcatgccg tgctacggac tggcagaaaa tgcgctggct gtcagcttct
     1201 ctgatgaagc ctgcggggtt gtggttaacg aagtggatcg cgacatcctc gaataccaag
     1261 gtaaagccgt cgcgccgggt gctgaaacac gcgccgtatc gactttcgtc aactgcggta
     1321 aagcgttgcc ggaacatggc attgaaatcc gcaatgaagc agatatacct gtcgcggaac
     1381 gtgtggtagg ccatatttgc atctccggcc ccagcctgat gagcgggtac tttggcgacc
     1441 aggtttcgca agacgagatt gccgcgacgg gctggttaga caccggcgac cttggttatc
     1501 tgctggacgg ttatctgtat gtcaccggac gcattaaaga tctgattatt attcgtggcc
     1561 gtaatatctg gccgcaggat attgaataca ttgcggaaca ggagccggaa attcattctg
     1621 gcgatgcgat tgcttttgtt accgcccagg aaaaaatcat tttgcagatc cagtgtcgga
     1681 tcagcgacga agcacgtcgc gggcagctta tccacgcgct ggcagctcgg atccaaagcg
     1741 aatttggcgt taccgcggat atcgatctgt tgccgcccca cagtattccc cgaacatcct
     1801 ccggcaagcc tgcccgtgcg gaagcgaaaa aacgttatca gaaggcttat gctgccagtc
     1861 ttcatgtgca ggaatccctg gcatgaatca aactgtcgcg gtgacgggcg ctaccgggtt
     1921 tatcggtaaa tatattattg ataacctgct cgcccgcggc tttcacgttc gcgcattgac
     1981 gcgtactgcc cgcgctcacg tcaatgataa tctcacttgg gtgcgcggtt cgctggaaga
     2041 tacacattcg cttagcgaac tggttgccgg agccagcgcg gtggtccatt gcgccgggca
     2101 agtgcgcggg cacaaagaag agatttttac ccgctgcaac gttgacggca gcctgcgcct
     2161 gatgcaagcg gcaaaagaga gcggcttttg ccaacgtttt ctgtttatct cttcgctggc
     2221 ggcgcgccat cccgaactct cctggtacgc aaattccaaa cacgtcgccg aacaacggct
     2281 gactgcaatg gctgacgaaa ttacgttggg cgtttttcgc ccgacagccg tgtatggtcc
     2341 cggcgataaa gagttaaaac cgctgtttga ctggatgctg cgcggtctgc tgccacgact
     2401 tggcacgcca gagacacagc tctcttttct tcacgtcacc gattttgcgc aagcagtggg
     2461 tcagtggtta agcgccgaaa ctgtacagac gcaaacctat gaattatgcg atggcgtcgc
     2521 cggaggctat gactggcaac gtgtccaaca gcttgttgcc gatgtccgct gtggttccgt
     2581 gcggatggtc ggtattcctc agccgctact cacctgcctt gcggatatca gtaccgcgtt
     2641 gagccgtctg gcgggtaaag aaccgatgct gactcgttcg aaaatccgtg aattaaccca
     2701 tgccgactgg tcggcaagta ataaccgtat ttctgaagat attaattggt ttcccgggat
     2761 tagcctggaa cacgcattac gcaacgggct attttgattg tcttgtttaa aggtgtgtta
     2821 acgtttctat ggtaaatcgt gaaatagtaa tggattacat cctttcctgt ttacaggatt
     2881 tggtcgaaaa cggtgtggaa attaagcccg acagcgatct cgtcaacgat cttggcctgg
     2941 agtcgatcaa agtgatggat ctcttaatga tgctggaaga cagattcgat atttccattc
     3001 ctattaatat tctgctggat gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat
     3061 ggctggagaa taactaatgg ggctatacga taaatatgcg cgccttgctg gcgagcgcct
     3121 gcaatttagt gacaacgggt taacaccatt tggcacctgt attgatgaag tgtattccgc
     3181 caccgaaggc cggatcggca ataaaaaagt gattctggcc gggaccaata attatctcgg
     3241 tttaaccttt aatcatgatg ctatccgcga aggtcaggca gcgttagcgg cccaaggcac
     3301 cggcaccacc ggttctcgta tggcaaacgg cagttatgcg ccgcatctgg cgttagagaa
     3361 agagattgcc gagtttttca atcgccctac cgctatcgtt ttttcaaccg gttataccgc
     3421 taatttaggc gtcatcagca cacttgccga ccataacgcc gtggtgttac tggatgccga
     3481 cagccacgcc agtatttatg acgcctgttc tctcggcggc gcggagatta ttcgtttccg
     3541 ccataatgac gtcaacgatc tggaacggcg catggtccgc ctcggtgaac gggcgaaaga
     3601 ggcgattatt atcgttgaag gcatctacag tatgctcggt gacgttgcgc cgctggcaga
     3661 aattgttgat atcaaacgtc gtctcggcgg atatttaatt gtcgatgaag cacattcgtt
     3721 tggcgtgtta ggcgcaacgg ggcgcggact ggttgaagcc gttggcgttg aagacgacgt
     3781 ggatattatc gtcggcacct tcagtaaaag tctggcctcg attggcggtt ttgctgtcgg
     3841 ttcggaagcg atggaagtgc tgcgctacgg cagccgcccg tatattttta ccgcctctcc
     3901 ttctccgtcc tgcattgcga ccgtgcgctc gtcgttaaga accattgcct cacaaccaga
     3961 gctgcggcaa aagctgatgg ataacgccaa ccatctgtat gacggtctgc aaaaactggg
     4021 ttacgagtta agttcacata ttagcccggt agtgccagta ataatcggtt cgaaagagga
     4081 aggtttacgc atctggcgcg agcttatttc gctgggcgtt tatgtgaatc ttatcctgcc
     4141 acctgccgcg cctgcaggca tcacgctgct gcgctgtagc gtaaacgctg cccacagtcg
     4201 tgagcaaatc gatgccatca ttcaggcatt tgctactctg aagcagtaat gtgaacccgc
     4261 cgtgttgttg cgccacggcg ggtttaatgt tgtctgtcat accaatacac caagctcaac
     4321 gccaccagca caaacgctac cggtaaaccg agcgcggtaa caggtgcgct caacgcaaat
     4381 agcaatccca gattgaccat gatttgatag ctgatccagg tgagtaaacc aacgatgacc
     4441 cctacagcga gacggctacc catccccggc gagcgcgggg cactaaaggt aaacggcaca
     4501 gccagcaaaa tcatcgccag ggttaagatg ggttgcccca gtttttccca cagtgcaagg
     4561 cggtattcgc tgctgggttg cccggtttcc tgcaaataat ggatgtaatg attaagctga
     4621 cggacagaaa aagtattccc cggcatcgat aactcttcaa gattcatact ggtgaaggcc
     4681 gattgccagg caagattatc taatgtttcc agcgtttctt tgccattcaa ccatttttta
     4741 taattcacac catgcaggat ccacgtttta tcgtcttcaa tggtggcact gcgcgcataa
     4801 atgtaggatt cgagcgagag atcgtcgcgg taatgaaaaa tctccacgcc caccggctgg
     4861 ccctgctcgt tcagcgattt caccgtcaca aattcattac cgcgcctggc ccaaagcata
     4921 ttgccgataa tgtcattgtc ctcccccaac gcggtggcag tcgatttgat ttgcagcgcc
     4981 tgttgctgca atggtgacgc cacccactca tcaatcgcgc ctaacgaaac agtccacagt
     5041 atccctgcaa ccagcgccac cagtgcaatg cggaagatag aaaaccccgt gctgcgaatg
     5101 gcggtaagct cactgttttt cgataactgc ccgagaccaa caatcccccc caataacgcg
     5161 ataaacggcg aaagttcgac cagtgtgcgc ggtaaggtca tcagcaccac cagcactgcc
     5221 tgagtccagc gataaccgcc cggactgaca tcatccagtt cgttaatcag gttgaaggtg
     5281 gtaaaaagcg gcagcaatag ccctgcggcg gcggcaaaac cgagaaagag gtgacggatt
     5341 aaatagcgac tgaaaacatt catcggggaa atttctgcag caaggagaag tcgcgtgcga
     5401 ccagcatcaa caggcccatt agcatgagtc cgggcactaa ccacaaacca ggaatgaggg
     5461 gaatcgcgcc gttagccacc agcgtacgac agacgtcgcc gccataaaaa atggcaacaa
     5521 acagcaacgt cagtggcagt agcgttgaaa atcgcccctg ccgcggtttt acccgactta
     5581 atgaaatggc cagcagcgcc atcaacaatg ctgtcaggcc acggctttgt cgccattgca
     5641 gttcggcatg atcggctgga aagacggagc gtgccagctc cgtgaccgat tttgctttgc
     5701 gtttgacgtt agggctttga tccagcggat tgagatgcaa ttgcagatta cggtagatct
     5761 gttcgttgtc gtcacgcccc tgatgatcga gaagataggc ggtaccgtta tgcaacataa
     5821 cggtcggttt ctcgggtgat gggtcaacca catcaaccga acgggcgcgg aaaatgcggg
     5881 ttcgattggc agtagaagta taaatcagcg cgtcagtcag gcgattatta tcctgatcaa
     5941 ccgtctgcga aaggatcatt cgtccgttat cgttagtgtt aaatttcttt gcccgcaact
     6001 gacgaacatc cagctccgac tgcgattgtt gctccagttg ataaatctgc gcataagccc
     6061 agggacgacc atacatcgac agaagggtga caaaaatact tagcggtatc gccagataaa
     6121 ggacggcttt gtacaaacgt cccggactgc cccccgccgc ggagatagcg gtaatttccg
     6181 agtcggtata catttgccct agcgtcacgc caactgacgc atacagtcca acaggtaaca
     6241 gcatctccag tgcaatcagc actttgtaga aaacgatatc cagcacaaca tcaagagcta
     6301 atgtcccatt tgccgcttcg gtcagataac gctgtgcgga gtagctggcg aaaataaaga
     6361 tcagaaaacc gacgataatc agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa
     6421 ccagcttcat ctcgctaacg ccctcaatcc tttgaatttt ttattcattg atcagtttct
     6481 gcgccagcgc aatgacatga tctacctctt ccggtgtcag gctgccgtcc tttgcccact
     6541 gaattcgccc ggctttattc aataccagaa tggttgaact ctgctccggc aattgccagg
     6601 caacgcgtcc cagaccattg ccgtcaataa cgaattgcgc ccacggatag cggcgtttat
     6661 ttttttcgat tttgccaacg acaaaatagc cagtaccaaa gatggcatca tcggtgttca
     6721 caatagtggt gggctgaaaa cgatcctgcg aaaagtttgc cgcttcaacc gctttaatta
     6781 acagtgagtt ctttttcttc gcagacttac gtccggcgat atactgaata atgcggactt
     6841 ttcccgtcaa ttgtgtgctg ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa
     6901 gcagctcgcc gccttcctgg atataaacag gagtaaccgt tttgccagtg acgaaattgt
     6961 gtgccgccgc gcctggcgct aagagtgcga tgatcagagc gacaatccaa cgagatggca
     7021 ttattgctcc ggatatagtg ggaaacgcga aataagcagt tctaaacgct ggcgaatatc
     7081 gctatccagc gccaccggtg catcatatga ccgacggtta tccatatcat ccgccaccat
     7141 gtcgtcgtgc ttatccccca tcagaccatt aatggcggcg agaaaacggg aaatcagtaa
     7201 cgcctcttta ttttcaggat caagcgatct taaggtgtcg taataagaaa cctgctggct
     7261 gatgattttt cgcacatcat gaaattccct gccagttagt tgcggtttag caataatctc
     7321 tttcaggatg tacatttgct tctggcggta ggcgataaaa ccgttcacgg tttctaactg
     7381 gctatggcgc aatgaatagt tcaccagcca ccagacaggg gagctcaaat aaacacgtaa
     7441 caggttaccg taaaacgaca caatattttt cttaccgtta cgaaagccat cctgaaaatg
     7501 cccaaaaaaa acggtcgttt tgccgaataa aaatccggcc tgatgcttct ttaaatacag
     7561 ccgttgcgca aaacgcagat gcttgtagcg cgctcgcatg tatttaaact gtttgcgctc
     7621 gtctgtcgtc agctcatcgc ctttcgcctg tttgcggatt aaacgcagta actcctgacg
     7681 attaacacca tccagccaga atttcaggca taacgcgtaa ttatcatgga tgatttcggt
     7741 ggtacaactt agtgcgatag tcggcggcaa actgacctgt gcatcaacaa catcatcttc
     7801 ttcaatcgca acaaatagac gctcgctcgc ctcaagcggg aaatattttc tcatcatcag
     7861 gatttctctc cggttgagtt ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtc
     7921 ggaattagcg agagcaccac atccagacat tgcccaccga agtaagcaaa tgcagccccc
     7981 agcacgccaa acagatgaca gaacatgaac agcaatacga tgtagccgat tgaggcgatg
     8041 gtttgcgcca cgagaaaagc gcgctgtttt cccgccatca atagcaatga ctcctgtggg
     8101 aagcccagca tcgaaatcac aattgcgccc agcatcacct gaatcaggtc atacgcttcg
     8161 agatacttaa cgccaaacac cagcgaaatg agcggattgc cgacgatcaa caccgcgagc
     8221 gccaccagaa taccgattcc gcccgccagt aaaccggact tcacgcctaa caaccacggt
     8281 ctggtggtgc gtggatctaa acgcatcacc tctgggtaaa aacttttgcc cagtaaaccc
     8341 gctggcgttc cggcggcatc gaagaatgtc atggcgattt taaataaccc ggcggcagcg
     8401 ggtcctaaca cgatccccac caacactgtg ctgcacgagt tacgcgccga ccagatggag
     8461 tgggcaatgt tggttgacca gacaaaactc cacgcgcctt taatgtgtcg ggcagactca
     8521 aacagattca atttgaaggc gttatggata tttctgcggc gtaattcgcg tgcggcaaac
     8581 caccagtaca tggtgccacc aaccagattc gacacgtacc aggcgataac aaaacccgca
     8641 aaaccaaagt caaaatacca ggctacgatg ctccccgctg cgcgcagaaa aggtttcgtc
     8701 gcctgctgta cagcaattaa gtcgaagcga tctaccgcac gcagaatgcc ggtcggcgta
     8761 gaggaagcca ttgaaggaat gagtgtgcaa tacagcgccg ccagccaaaa gctttggtca
     8821 tccagaccta atgaatggga aagaaaaggg agtaaggcaa tgccgacgac aatcgccacc
     8881 gcgccgctga caatatccag cgaaaaggaa aatgagacaa cattgcgaaa ttgctgcgga
     8941 ttattgttgg tcaaagccgg cgttccgtac tgaaccacca gctgccatgt ctgaaactta
     9001 ataaaatcgc tgatcgactt ggcgtacgat tgcacaatta ccagtacgcc aaacatggcg
     9061 ggcgtcatcc ctttaccggc acacgagagc gccaacagcc ccagcaaagc gctcacaaca
     9121 ttactggagc ctaactaggc gctattgcga ataatggtgc gaaacgcgcc atctgcaaac
     9181 caatgtttga tgttaaaacc cgccaattca gcctgacctt atcgttgata gtaaaaagta
     9241 tcccgcctgc cttaagttaa acttcggcgg tcagaaacga tggcaaccag agaaaccgcc
     9301 ttctgtgcct gttccagcac ctcgctgtaa ggcgctctgg aatcaatctc aagaattttt
     9361 gtgccgttat agccaatcgt cgacataaca ccgattttgt cctgcagctc ggcatagtca
     9421 tggtcaggct tgcgggagat ggcagtatca atatcaatcc ccaagcgaat gattaattca
     9481 ggacggtatt gcgccatttt ttggtataaa cgccgttcac gctgcgccag aaacctgctg
     9541 attttcccgg tcgcacgttc gacgccaatc cccggtccgt cataataaaa gcccgaaatt
     9601 tcagcctgcg ggaagcgatc gctgaccacc agaacgccac tttgcgccag tcgctgaacc
     9661 ttgcgcagat tcgccattct tcgcagcgag aagcagtaca taatcaccgc cgcccatagc
     9721 gccggagatt tggttttcat gctttgggtt ttcgaggatt tggccgccag tcgccgttcg
     9781 agccagacac caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc
     9841 cccagatagc gccgctcggt ttgccagtgt tgttgcagcg atttcaccag gtcattggtc
     9901 agtgtggatt taccggtgcc atcacacccg accaccgcaa tcagccccgg aatgtagttg
     9961 ggctgcggcg cggttgtact cttaacagtt tgtgtttgta gtgcatccat tcgtttaatc
    10021 atcccctatc cattttggtg atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg
    10081 cagaatttca tcggctggtt gccgaccatc cagttcaagg atcctggcgc cattgaatgt
    10141 cagttgcggc gtaacggcga ttttttcctg taacgctgcc agttggtggt caggtttacg
    10201 cgcaaacgcg gtttgttcat caatgccaag acgaatcaac aatacaggca aataagacgc
    10261 catccattgg tacagcttca gctcacgctg ccttaacatt tttatccaac cgttaccgcc
    10321 cgtggttttt gccaattgcg ggccatcaaa gcggaaaccc ggcacttcaa cctgcgggta
    10381 gcggtcggtg atgagcagat agccttgctg gtttttacac aacattttgc gaaacttgta
    10441 cgcccgccag caggaaagca gatagataac cagcgcagta atattgccag gcggtgttga
    10501 gggcttttcg tgcacatgtg ccgctttact tcgcaaataa cgcccaaaag gtgcgccaat
    10561 aacaggtagc tgtgaaatcc attcgccaat tcgcccggac gattgcccga gataaatgtg
    10621 ttctgttggc attcttgatg ccagttcatt taccaggctt gccgtgaggg tcgatttacc
    10681 tgaaccatca catcctacaa tggcaataac acgcactggc gtggaattaa ttattgacat
    10741 attacgttga ttcacgaaaa acccggcagc aaataatgta ttgaatatta cgtggtccgt
    10801 gctcagaata tccgttcaac cttattgtga caatggttaa ttgtgacatt gacctgaatt
//
LOCUS       DAJOTB010000150        10852 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJOTB010000150 DAJOTB010000000
VERSION     DAJOTB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.150"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            33..647
                     /gene="evgA"
                     /locus_tag="OHN28_003959"
     CDS             33..647
                     /gene="evgA"
                     /locus_tag="OHN28_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCQ2299261.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            652..4245
                     /gene="evgS"
                     /locus_tag="OHN28_003960"
     CDS             652..4245
                     /gene="evgS"
                     /locus_tag="OHN28_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCQ2299262.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRKIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4301..5446)
                     /gene="yfdE"
                     /locus_tag="OHN28_003961"
     CDS             complement(4301..5446)
                     /gene="yfdE"
                     /locus_tag="OHN28_003961"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCQ2299263.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5520..6464)
                     /gene="yfdV"
                     /locus_tag="OHN28_003962"
     CDS             complement(5520..6464)
                     /gene="yfdV"
                     /locus_tag="OHN28_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCQ2299264.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6534..8228)
                     /gene="oxc"
                     /locus_tag="OHN28_003963"
     CDS             complement(6534..8228)
                     /gene="oxc"
                     /locus_tag="OHN28_003963"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCQ2299265.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8282..9532)
                     /gene="frc"
                     /locus_tag="OHN28_003964"
     CDS             complement(8282..9532)
                     /gene="frc"
                     /locus_tag="OHN28_003964"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCQ2299266.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10044..10676)
                     /locus_tag="OHN28_003965"
     CDS             complement(10044..10676)
                     /locus_tag="OHN28_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCQ2299267.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3175 a         2463 c         2228 g         2986 t
ORIGIN      
        1 agggaaatgc ttcattgcaa agggaataat ctatgaacgc aataattatt gatgaccatc
       61 ctcttgctat cgcagcaatt cgtaatttat tgatcaaaaa cgatattgaa atcttagcag
      121 agttgactga aggcggaagc gccgttcagc gggtggaaac acttaagcct gatatcgtca
      181 tcattgatgt cgatatcccc ggagttaacg gtatccaggt gttagaaacg ctgagaaagc
      241 gccaatatag cggaattatt attattgtct ccgctaaaaa tgaccatttt tacgggaaac
      301 attgtgctga tgctggcgct aatggtttcg tgagtaaaaa agaaggcatg aacaatatca
      361 ttgcagctat tgaagctgca aaaaatggct actgctattt ccccttctct ctcaaccggt
      421 ttgttggaag tttaacgtcc gaccagcaaa aactcgactc cttatcgaaa caagaaatta
      481 gtgtcatgcg gtatattctt gatggcaagg ataataatga cattgctgaa aaaatgttca
      541 tcagcaacaa aactgtcagc acttataaaa gtcgcttgat ggaaaaatta gaatgcaaat
      601 cactgatgga tctttacaca ttcgcacaac gtaacaaaat cggctaacca catgaagttt
      661 ttaccctata tttttcttct ctgttgtggt ctttggtcga ccataagttt cgcagacgga
      721 gattacatcg aatatcgtgg catcagtagt aacaaccgtg tcacacttga tccactacgt
      781 ctgagcaaca aggaattacg ttggctagcg agcaaaaaaa atcttgtgat tgcagtacac
      841 aagtcccaaa cagctacgtt gttgcatacc gattcgcagc aacggattcg tggtattaat
      901 gctgattatt taaatctttt aaaaagagcg ttaaatatca aattaacact ccgggaatac
      961 gcagatcatc aaaaagcaat ggacgcgctg gaagacggtg aagttgatat agtgttatca
     1021 catttagttg cttcgccgcc tcttaatgat gacattgctg caactaatcc tctgataatt
     1081 acctttccgg cgctggtcac tacccttcac gattcaatgc gaccgcttac ctcatcaaaa
     1141 ccagtaaata ttgctcgagt agcaaattat cccccagacg aggtaattca tcaatcattt
     1201 ccaaaagcaa caattatctc ttttacaaat ttatatcagg cattagcatc cgtctcagcc
     1261 ggacagaatg attactttat tggtagtaac atcattacca gcagtatgat ttcccgttat
     1321 tttactcact ccttaaatgt agtgaagtat tataactcac cgcgtcaata taacttttta
     1381 ttaaccagaa aagattcaat cgttcttaat gaagtactca atcgatttgt tgatgcttta
     1441 acaaatgaag ttcgctatga agtatcacaa aattggcttg atacaggaaa cctggccttt
     1501 ctgaacaaac cattagaact cactgaacat gaaaaacagt ggattaagca gcatcccgat
     1561 ttaaaggtgc tggaaaatcc ttactcgcca ccctattcta tgacagatga aactggctca
     1621 gttcggggcg tgatgggtga tattcttaat attattacct tgcaaacagg tttaaatttt
     1681 tctccgatca ccgtttcaca caatattcat gctgggacac agcttaaccc cggtggatgg
     1741 gatatattgc ccgccgctat ttatagtgaa gatcgagaaa ataatgtttc atttgctgaa
     1801 gtcttcataa caacgcctta cgtttttgtc atgcaaaaag cgcctgacag tgaacaaaca
     1861 ttaaaaaaag gaatgaaagt tgccattcca tattattatg agcttcattc gcaattaaaa
     1921 gagatgtatc cggaggtaga gtggataaaa gtcgataacg ccagcgctgc atttcacaag
     1981 gtcaaggaag gtgaacttga tgctctggtc gcgacacagt taaattcgcg ttacatgatc
     2041 gaccattact atcctaatga actttatcat tttcttattc ccggcgttca gaatgcatca
     2101 ctttcgttcg cttttcctcg cggagaaccg gaacttaagg atattattaa taaagcactg
     2161 aatgcaattc ccccaagcga agttctgcgc ctgaccgaaa aatggattaa aatgcccaat
     2221 gtgaccattg acacatggga cctctatagc gagcaatttt atattgttac gacattatcc
     2281 gttttattag ttggcagtag ccttttatgg ggattctacc tgttacgctc agttcgtcgt
     2341 cgtaaagtta ttcagggtga tttagaaaac caaatatcat tccggaaagc gctctcggat
     2401 tccttaccta atccaactta tgttgtaaac tggcaaggta atgtcattag tcacaatagt
     2461 gcatttgaac attatttcac tgctgattac tataaaaatg caatgttacc attagaaaac
     2521 agtgaatccc cctttaaaga tgttttttct aatacgcatg aagtcacagc agaaacgaaa
     2581 gaaaaccgaa aaatatacac acaggtattt gaaattgata atggcatcga gaaaagatgc
     2641 attaatcact ggcatacctt atgtaatctg ccagcaagcg aacatgctgt ttatatttgt
     2701 ggttggcaag atattaccga gacgcgtgat ttaattcatg cactcgaagt agagagaaat
     2761 aaagcgatca atgcaactgt cgcaaaaagc cagtttctgg caacaatgag tcacgaaata
     2821 agaacaccaa taagctccat tatgggcttc ctggaacttc tgtcgggttc tggtcttagc
     2881 aaggaacaac gggtggaggc gatttcactt gcctacgcca ccggacaatc actcctcggc
     2941 ttaattggtg aaatccttga tgtcgacaaa attgaatcgg gtaactatca acttcaacca
     3001 caatgggtcg atatccctac tttagtccag aacacttgtc actctttcgc tgcgattgct
     3061 gcaagcaaat cgatcgcatt aagttgcagc agtacgcttc ctgaacgcta cctggttaag
     3121 atcgaccctc aggcgtttaa gcaggtctta tctaatttac tgagtaatgc tctcaaattt
     3181 actaccgagg gggcagtaaa aattacgacc tccctgggtc acattgatga caaccacgct
     3241 gttatcaaaa tgacgattat ggattctgga agtggattat cgcaggaaga acaacaacaa
     3301 ctgtttaaac gctatagcca aacaagtgca ggtcgtcagc aaacaggttc tggtttaggc
     3361 ttaatgatct gcaaagaatt aattaaaaac atgcagggcg atttgtcatt agaaagtcat
     3421 ccaggcatag gaacgatatt tacgatcaca atcccggtag aaattaccca acaagtggcg
     3481 gctgtcgagg caaaagcaga acaacctata acactacctg aaaagttgag catattaatc
     3541 gcggatgatc atccgaccaa caggctatta ctcaaacgcc agctaaatct attaggatat
     3601 gatgttgatg aagccactga tggtgtgcaa gcgctacaca aaatcagtat gcaacattat
     3661 gatctgctta ttactgacgt taatatgccg aatatggatg gttttgagtt gactcgcaaa
     3721 ctccgtgagc aaaattcttc cttacccatc tgggggctta cagccaacgc acaggctaac
     3781 gaacgtgaaa aagggttaaa ttgcggcatg aacttatgtt tgttcaaacc gttgactctg
     3841 gatgtactga aaacacattt aagtcagtta caccaggttg cgcatattgc acctcagtat
     3901 cgccaccttg atatcgaggc cctgaagaat aatacggcga atgatctaca actgatgcag
     3961 gagattctca tgactttcca gcatgaaacg cataaagatc tacccgctgc gtttcatgca
     4021 ctagaagctg gcgataacag aactttccat cagtgtattc atcgcatcca cggtgcggct
     4081 aacatcctga atttgcaaaa gttgattaat attagccacc agttagaaat aacacctgtt
     4141 tcagatgaca gtaagcctga aattcttcag ttgctgaact ctgtaaaaga acacattgca
     4201 gagctggacc aggagattac tgttttctgt cagcaaaatg actaaatagc ggctcccaca
     4261 atgttcaaat gtgggagcta tttaccagca catcttacat ttatgatgag aactcctggc
     4321 gaatttgttc cccatgctgg tcgagcgttg ccgctcccgg cataacatgc gggtccgcgc
     4381 agccgctgat ttttatcgga ttacccggca tcattattcc cccggcttca atcaacatat
     4441 ttctcgcctg agtttgtggt aaattaatgg cctcagccac acttaataac ggcgcgacgg
     4501 gcacaccaac ttcatgtatt ctggctaacc aaacttcagc tacctgcgtt tttaacgtcc
     4561 gctcaatata ttctttaaga atagcctggt tatgtacgcg taaaatattg ctgctaaatc
     4621 ggggatcatt aaccagttcc gtaagctcca gtgcctggca taacgcagaa aaaagcttgt
     4681 cattaccaca acaaatcgta atcggcttat cctgagtatc gaaaacatca aaaggtgcca
     4741 tgtagggatg gcgatttccc aggcgttgtg gtgacttccc agtcgcgata tatgccatca
     4801 gtccatgctc cagaaaactc agagtggcat caaacatcgc tatatcgaca tgcgcccctc
     4861 tctggctctt ttcgcggcca taaagtgcac tcactattcc actgaataaa taaacaccgc
     4921 cacagagatc cgcaagagag gtaccaacgc gcactggcgg agcatcaggg tatcctgttt
     4981 ccatcattat cccgctcatt gcctgaatga tggtatcgta ggcaggagca tcttttagcg
     5041 gaccggtatg tccgaaacct gacgatgaag catatataag gcgcgggttg atttcttgta
     5101 acctttccca tgaaaacccg agtttttcca ttgtacctgg gcgaaaattc tcagctaata
     5161 catcagcttg tttaagcata tttataaata tacttttatc gtgatcattc tttaaatcaa
     5221 gaaccacact ctctttgcca tgattaataa aactgtaata gagtgactgt ccatccacat
     5281 agggaccaaa tgtgcgggta tcatcaccat gacccggtgg ctcaacttta attacccttg
     5341 cgcccatatt acaaagaagt tgagttccga aaggcccatt aaggacatgc gtcatatcga
     5401 taactaataa gccttcaaac ggccctttgc attcattatt tgtcatttgc ttatgccttg
     5461 aaaaaagccg aaggtgaaaa gacacttcgg catttatgta aaatcaataa catgataaat
     5521 tatgaaacca gtcgactgac ataaatccac aaaggagccg tgacgacaaa acccagtacg
     5581 ctcacagcca atgacgctgt accagtacgg gtgtagacat taaaccggct ggcaataatg
     5641 attccggaga atgccggcgg taatgcgcct gccagtacca tcatctgcaa atgttcgcta
     5701 ttcaaatgac atgccatacc gacgagaagc agcgccagtg gcatcagaat cagcttcagg
     5761 aaagtgttat aagcaatttc ggcactgaac tcgaatttat gtgcagccag agtcaacccc
     5821 gcagcgaata ccgctacccc tgagttagcc ttcgcaatca gattaaaggt tgggtcccat
     5881 gctgccggaa tttttacccc aaccagcacc aggatcgttg ccagaacagg tgcccatact
     5941 actggctcct ttgccgcaga aattaatgcg ctcagattac tattcttctt accatccgct
     6001 cctgaggaag gattcagcaa atacagacca ataggaatag taattgcgtt aacaataata
     6061 gaaataattg ctaccactaa acctgttgat acggaatcac cataaatagg atcgagaact
     6121 gcaaacccca ggaatccaat ggtaggtgaa cctgcaatta atgcacatac tgctgcttct
     6181 gcatgggtac gtttaaaaaa tttgtagcaa ccgaaccagg agaaaaagaa acatccgaca
     6241 ataaccacaa gtgatacaag ggtcaggcga gtgtccgcaa aaatcatttc cctgtttgcc
     6301 ctggtaatag aaacaaatag agccgcagga agagcgtagt ttaataccag tttattaaat
     6361 gcccgggctt gatcttctga aaaagtttct cgtctgccgc taaaataacc caacagcatg
     6421 attacgataa taggcaataa atcgccaata aaaaatgtta gcataatagt atttccgata
     6481 agggtcccct caccatcgca taataagttg gcaagaggag acgatgtcag tatttaatta
     6541 ccagcgactt gttttgggtt aagtttggta atatggccac tttcagttcc tgctgctggg
     6601 tcgatgacca cattaataat ggtcggtttg cgcgactgaa ttccggtggt taaagcatga
     6661 cgaagttcat ctgtcgtggt gacgttatag ccaacgccac gaaacgcatc cattaatttg
     6721 tcataccttg catggtgcaa cagatccgtt ggcgatggtg caccagcgcc gctgaggtca
     6781 acaccgtctc ctctgtagat gccgccatta ttaaaaataa cgatcgtcac cggcaggtta
     6841 tatcgacaaa tcgtttcaat ctccatccca ctgaaaccaa aagcactatc accttcaata
     6901 gcgacaaccg gagaaccaga ggtcacgcta gcaccgatgg catagcccat accgatgccc
     6961 atgacacccc aggtgccaca atccagacga cgacgtggtt tatacatatc aataatattt
     7021 cgtgcattat ccagggtatt tgccccttca ttaactaaat aaatatcctg gttctcgcgc
     7081 aaaacatcgc gcacggcact caatgcatta aagtaattta atggttgggt atctgtactt
     7141 aacttttcat gcattttttg tgcattttgc tgcttgtgga tatttaaaat atcacgccac
     7201 accagtggag tcgtaaatgt gttttgtttc aattctgcca gcataccttg catactggat
     7261 gcaatatcgc caacgactgg cacagcaatg gggcggttgc tgtcaatttc ctgcggttca
     7321 atatccagtt gaataaactg tgtatctgcc gcccatcctt ttttaccgtg tgccagtaac
     7381 caattcagtc gtgcaccaac aagcatgaca acgtcagcat ttgccagggc aaacgaacgc
     7441 gcagctgccg cagagagtgg atgcgtatct tcgaggatcc ctttcgccat agacattggc
     7501 aggaatggaa tctgggcact ttcaataaat tcacgaagct gttcatcaga ttgtgaatac
     7561 gccgcgcctt tgccaagaat aattaatggt cgttcagctt ttgctaaaag cgaaattgcg
     7621 ctagtgactg acttcgggca tggcaataat gctggcgacg gattttcaac tttaacaatc
     7681 gtggttaacg cttcgtcttt ttccatcgtc gcggccagga catttgctgg caaatcaaga
     7741 taaacaccgc caggacgacc cgatactgag acccggatag cgcgtgccaa tgcaatgcca
     7801 agatcctgcg gctgattaac gcgaaatgct gctttggcat acggttttgc cgcattcatt
     7861 tggtccagct cttcataatc accttgctgg agatcgacga tcgcgcggtc gctggagccg
     7921 ctaatcatga tcatcggaaa accatttacc gttgcattgg ccaatgcggt caaaccgttg
     7981 aggaatcctg gcgcagacac tgtcaggcag atccctggtt tttgggtaag aaaaccgctt
     8041 gctgcagcgg cataacctgc cgactgctca tgacgaaaac caatataacg aatgccttcc
     8101 gcctgggcat ggcgcgccat atccgtcaca ggaataccta caacaccata aatagtgtca
     8161 atattattct gttttaatgc ttcaacgatg atatgcatac catctgtcat ttgaagttga
     8221 tctgacatga ttaataacct cgaaattttt ataaattggg agccagaagc ccccgttaat
     8281 atcagatggc gtggttttgc ttcattgcag caatttcatc gtcgctataa cccagctcct
     8341 gcaatacagc agcggtatgt tcacctaata gcggcgcagc tttaatatcc ggcgtaaagg
     8401 cagagaattt cattggacaa ccaactgtca gatattttcc acgcaacggt tgttccactt
     8461 cgacaacact gccactttgg cgcaaagagg gatcaagtga aatttctttc atacttaaaa
     8521 ccggtgcgca aggaatatca aactgagtca aataggccac agcttcatgt ttatcaatag
     8581 tgacagtgta tttttcgatt tcagcaaaaa tatcgaaaat atgtggctgt cgggcatggg
     8641 ctgtactgta tgccggatcg gtaatccatt ctggtttgcc gatggcttta caggtgtttt
     8701 cccagttttg ctcctgaata gtgaaataaa tataggcgtt aggatcggtt tcccagcctt
     8761 tacatttcag gatccagcca ggctgaccac caccacccgc attaccaccg cggggaactg
     8821 catcaccaaa tgtgccattc ggatactgcg ggtattcttc cagataaccc aatttatcga
     8881 gacgctgctg gtcgcgtaat ttcacgcggc aaaggttcaa cacggcatcc tgcattgaca
     8941 tggtcactcg ttgcccacgc cccgtttttt cgcgatgcag caaagcagca agtaaaccga
     9001 tcagcaaatg cattccggtg ttgctgtcac ccaacgctgc agcgcttacc agcggcggac
     9061 cgtcccaaaa acctgtagta gatgccgcgc cacccgctgc ctgagcaacg ttttcatagg
     9121 cttttacatt cacataaggc gaacactcat caaacccttt gatcgaacca aaaatcagac
     9181 gtggattgat ttcttgaata tgctcccagg tgaagcccat atgatcaatg gcccctggat
     9241 gaaagttctc gactaagata tcagcttcgc ggatcagctt ttccattacc tctttgcctt
     9301 ccgctgtttt ggtatttaat tcaatagaac gtttgttact gttaagcatg gtgaagtaaa
     9361 gcgcatcgat atcaggaata tctcgcagct ggtggcgcgt tacgtcacca acgccgggac
     9421 gttcaatttt aatgacgtca gcgccaaacc aggccagcat ttgagtacaa gatgggccag
     9481 attgcacacc ggtgaaatcg agaactttaa ttccttgaag tggagttgac atagcaatac
     9541 ctattagtta attaaatatg tgttaattta gcgtttattt aaggcataac gcctctataa
     9601 ataatctgca aaatatcgtc gtagctgcac tcgatatttt taaattcctg tatcaagtgc
     9661 tgaaaatcat ttcatcgcta cttccaattc ctttctgcat ggtaagtagt gaggacgaaa
     9721 agaagaatat aataaatatt ggttcgcggt aatcagagag agccgcgaca tttgtgatgt
     9781 tattcttaca actgtaagaa gtgtcagtga tttacaagta aggttatgat tacactattt
     9841 aaagcgtacc ataaaatata acataataaa tgatttgtga taatatggaa ttattgcggt
     9901 ttactttgag gaagataaaa tgcgagtata tagaaccgcg agcgccaaat aattttatga
     9961 ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta
    10021 cctcaatata ttgcctgtca ggtttagttc acaaacagtg cttcgctatc aacgatgatt
    10081 ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt
    10141 ttctgcgcat cagcaagtgc attacgagtc tgtttgagcg gcatcagata ttggttttcc
    10201 attacgccaa caccggccag gcgcagctct tccattgcgc cctttttatc ccctttcgcc
    10261 attttttcgt tggcaatttt tatcgcggct tctttttcgg gtgtcgctac ataactctca
    10321 gaaattccga cagatgcatt aatgacaata tattggtcat cattgacgtt ggtcttttta
    10381 cctggcttag cgaactttgc ccattcggta ctatcatctg acagcaaagc ggaagcttca
    10441 ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca gagccaggcg cgctacctgc
    10501 acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg
    10561 gtttgcttaa cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
    10621 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
    10681 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
    10741 aaatcagaga gcaacggtca ttttgaaagg gttatcttac aactgtagtt attatcgcaa
    10801 agccgctttt agtcttacag ttgtaggaat ctgcttcctg tcgcagagaa ct
//
LOCUS       DAJOTB010000151        10759 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJOTB010000151 DAJOTB010000000
VERSION     DAJOTB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.151"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..302)
                     /gene="papA"
                     /locus_tag="OHN28_003966"
     CDS             complement(<1..302)
                     /gene="papA"
                     /locus_tag="OHN28_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
                     /protein_id="HCQ2299268.1"
                     /translation="MIKSVIAGAVAMAVVSFGVNAAPTIPQGQGKVTFNGTVVDAPCS
                     ISQKSADQSIDFGQLSKSFLEAGGTSKPMDLDIELVNCDITAFKQGQALMNPLMIF"
     gene            complement(509..823)
                     /locus_tag="OHN28_003967"
     CDS             complement(509..823)
                     /locus_tag="OHN28_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon regulatory protein
                     PapB"
                     /protein_id="HCQ2299269.1"
                     /translation="MAHHEVISRSGNAFLLNIRESVLLPGSMSEMHFFLLIGISSIHS
                     DRVILAMKDYLVGGHSRKEVCEKYQMNNGYFSTTLGRLIRLNALAARLAPYYTDESSA
                     FD"
     gene            1246..1524
                     /locus_tag="OHN28_003968"
     CDS             1246..1524
                     /locus_tag="OHN28_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HCQ2299270.1"
                     /translation="MSEYMKNEILEFLNRHDGGKTAEIAEALAVTDYQARYYLLLLEK
                     AGMVQRSPLRRGMATYWLLKGEKQAGQSCSLCKLCRPVVIRSVTGLAR"
     gene            1458..2114
                     /locus_tag="OHN28_003969"
                     /pseudo
     CDS             1458..2114
                     /locus_tag="OHN28_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            complement(2290..2592)
                     /locus_tag="OHN28_003970"
     CDS             complement(2290..2592)
                     /locus_tag="OHN28_003970"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299271.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2679..3494)
                     /gene="sul2"
                     /locus_tag="OHN28_003971"
     CDS             complement(2679..3494)
                     /gene="sul2"
                     /locus_tag="OHN28_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCQ2299272.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(3735..4301)
                     /locus_tag="OHN28_003972"
     CDS             complement(3735..4301)
                     /locus_tag="OHN28_003972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HCQ2299273.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVDANSSSLLIG
                     ENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLGRDAGQAESK
                     YGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVSVGAGPEGYG
                     ESVKGAPETHSIVNKTFLSFIFNGLFSC"
     gene            complement(5125..5238)
                     /locus_tag="OHN28_003973"
     CDS             complement(5125..5238)
                     /locus_tag="OHN28_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2299274.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNVR"
     gene            complement(5501..5677)
                     /locus_tag="OHN28_003974"
                     /pseudo
     CDS             complement(5501..5677)
                     /locus_tag="OHN28_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            5737..6428
                     /locus_tag="OHN28_003975"
                     /pseudo
     CDS             5737..6428
                     /locus_tag="OHN28_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            6831..7022
                     /locus_tag="OHN28_003976"
     CDS             6831..7022
                     /locus_tag="OHN28_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCQ2299275.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTDTAYKMKAGDQIPQLNDK
                     QKPADKTPWRHDKTPEQKP"
     gene            7091..7339
                     /locus_tag="OHN28_003977"
     CDS             7091..7339
                     /locus_tag="OHN28_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCQ2299276.1"
                     /translation="MGIPDDLIQDIAIRELAFGAGTLHAAVASYVQSPRYYRALIAGG
                     ARYNLNGQPCGEVTPQEQKEAETRLMMLNDRRKARKPR"
     gene            complement(7358..7906)
                     /locus_tag="OHN28_003978"
     CDS             complement(7358..7906)
                     /locus_tag="OHN28_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299277.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEECSQLVDLLQQTWQVISAGGQCRVEKRFQVVRGDTSGQYVALKTVALSL
                     GLPVVIAITHRPVQRCTLITAQ"
     gene            complement(8172..8408)
                     /locus_tag="OHN28_003979"
     CDS             complement(8172..8408)
                     /locus_tag="OHN28_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2299278.1"
                     /translation="MLTSMTGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQHRIEASRGAAA"
     gene            complement(8477..9049)
                     /locus_tag="OHN28_003980"
     CDS             complement(8477..9049)
                     /locus_tag="OHN28_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299279.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGVLNRVLREQSCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFPSPGEAAPYQKQITRDVVLDSCSPDCEERLKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            complement(9308..9874)
                     /locus_tag="OHN28_003981"
     CDS             complement(9308..9874)
                     /locus_tag="OHN28_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2299280.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFAERIAECWQAITYNCGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
BASE COUNT         3025 a         2610 c         2509 g         2615 t
ORIGIN      
        1 ataaaaatca ttaggggatt catcagtgct tgaccttgtt taaaagctgt aatatcgcaa
       61 tttactaact caatatctaa atccattggc ttggatgtac ctcctgcctc aaggaagctt
      121 tttgaaagct gtccaaaatc aatagactga tcagctgatt tctgagaaat gctgcatgga
      181 gcatcaacaa cagttccgtt aaaagttact ttaccctgcc cctgtggaat agttggagca
      241 gcatttacac caaaagacac cactgccata gctaccgcac cggcaataac cgacttaatc
      301 ataaataaca acctcttttt cattactgaa cacaccagag atatctggca atttaaaatg
      361 acccgattac ctttaataac cagatcaata caaataatat tctcgtattt tcagggcagg
      421 ttatttatcg acagaatact gatataacct gtctgctttc tgataaggac ttctttttat
      481 ctgtaagata actccggaat gccatcattc agtcaaatgc cgacgactca tctgtataat
      541 aaggtgcaag ccttgctgca agagcattca gccgtataag tctcccaagt gttgtactga
      601 aatacccatt attcatctgg tatttctcgc agacctcctt acgggagtgc ccacctacca
      661 gatagtcctt catagccaga atgaccctgt cactgtgaat agaagaaata cctatcagta
      721 aaaaaaaatg catttcagac atagagccgg gcaacagtac gctctcgcgt atattcagca
      781 aaaacgcatt tcctgaccga ctgatgactt catgatgcgc catgtttccc ccttctgtcg
      841 ggcccctgga tatatgcttc cggcccggaa atactcttca cgcaataagt taaatttagt
      901 tttttatgtt gtaaatattg atttaaacga tcttttaacc cacaaaataa aattaaacta
      961 gaataaaaca ctaataataa gaatgcgctc agataaaaac atcatagtaa attgaattta
     1021 cagcataaaa gatcgtctaa atgcattttt acggactttc ttttcacaga aaaataaaac
     1081 gcaaaatgtg atacatatca aataaagcaa ataaaaagca aattcactaa attgcacaaa
     1141 taaagcaaac acagcaaaca cagcaaacac agagaataaa aatacctttc cgttgataac
     1201 agaaagcatg atttttatta ttcgaagaaa ctttgttaca gtgagatgag tgaatatatg
     1261 aagaatgaaa tactggaatt tctgaacagg catgatggag ggaaaacggc agaaattgcg
     1321 gaggcgctgg cggtaacgga ttaccaggcc cgctattacc tgttattact ggaaaaagca
     1381 ggtatggttc agcgctcacc attaagacgg ggcatggcaa catactggct tctgaaagga
     1441 gagaagcagg cggggcagag ttgttcactt tgcaaactct gccgaccagt ggtgatccgt
     1501 tcggtgacgg gattggcaag gtagccgggt ccagcctgca cgccggcgtg gcggccaggg
     1561 ccgatgaacg caagaagctc gaacggctgt gccggtacat cagccgcccg gcggtatccg
     1621 agaagcggct gtcgttaaca cgaggcggca acgtgcgcta ccagctcaag acgccgtacc
     1681 gggacggcac cacgcacgtc attttcgaac cattggattt cattgcaagg ctggccgccc
     1741 tggtaccgaa gcccagagtc aacctaaccc gcttccacgg ggtgttcgca cccaacagtc
     1801 ggcaccgggc gttggtcacg ccggcaaaac ggggcagggg caacaaggtc agggtggctg
     1861 atgaaccggc aacaccagca caacggcgag cgtcgatgac atgggcgcaa cggctcaagc
     1921 gtgttttcaa tatcgacatc gagacctgca gcggctgcgg cggcgccatg aaagtcatcg
     1981 cctgcattga agaccctata gtgatcaagc agatccttga tcacctgaag cacaaagccg
     2041 aaaccagcgg gaccagggcg ttacccgaaa gccgggcgcc accggctgag ctgctcctgg
     2101 gtctgtttga ctgacgagcc tgaaggccaa cgataccaat caaaatgctg cgttcacagc
     2161 gccgcggcag ggatccgccg tgctggttgt cggaaaagga gccgctagtg ggaaagagga
     2221 gggtaaattt tcagcgttgc tggctccccg tcagccggat tgggttgcat cgcaggggtg
     2281 tcgaaagagt caactgcggt ccaaagctgt tggacttggg tgaaaagggc gtttattctt
     2341 cctatacgtt gtcggcagcg ggccaaaaag gaatacgtcc atgcccatcg aggtgaaacc
     2401 ggctgtgagc gcgggttcaa gcatatagcc cgacaggcgc gtatccttgc cgatcacgac
     2461 acgatggcgg tggtcaccgc gacgaaagac acggccagcc gccatgccga cgcgcaaggc
     2521 ggtttccgcc gtcatcgcgc cttcgttggc tttgccacga ataccgtctg tgccgaaata
     2581 tttgcgcacc ataaggtcga ttatcctgtc gtcgggtcgc cctcaaaggg gacatgcctg
     2641 ctgaaccgcg aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct
     2701 ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg tgtgcggatg
     2761 aagtcagctc cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc
     2821 cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc
     2881 ggcaaatcga agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg
     2941 ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata
     3001 ccggctcccg tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg
     3061 tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg
     3121 agtttggcag atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata
     3181 tcattgagat aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg
     3241 tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg
     3301 atttctgtgt cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg
     3361 atcacatctg ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc
     3421 agataccggc ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg
     3481 agcgatttat tcatgggggc ttctataata ataataatcg agcatgagtc tcatacggat
     3541 gctcgggtcg aaagggaatc cccaggcgag taacctgttt gcggtgatcc attagctgca
     3601 ggagcagaat agcatacatc tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg
     3661 cagcgctcag taggcaattt ttcaaaatat tgttaagcct tttctgagca tggtattttt
     3721 catggtatta ccaattagca ggaaaataag ccattgaata taaaagataa aaatgtcttg
     3781 tttacaatag agtgggtttc cggtgctcct ttgacactct ctccatatcc ctcaggcccg
     3841 gcaccaacag ataccacgtt attttctccc gacacctgtg agccatggcc cagagctaca
     3901 gagcctgaag aattttctat tccagcccat cccccaatag caacactctg ctgtgaatta
     3961 agtacctcag cgctcttacc tatggcaaca ccatattttg actcagcctg accggcatcc
     4021 cttcccaggg tgacactgtg tggagaatta tacactcccg aaaaagagcc aaactgaaga
     4081 cttcctggtg aatccatgtt aaaagcgcca cctcccaaaa ggaaagtgtt attagctgta
     4141 ccagttgaat tcgtaataaa agagttttct ccaatcagta agctggaaga attagcatca
     4201 acaggccctg tcaaaacagc ctgagcctga gccgagagca gcagggccac actcactgcc
     4261 agcgcgcttt tctttaaaaa agcatcacgt cttttaagca ttctgttatt ctcctgtatt
     4321 attacttcac cagccagaac ggtagtgcgg agcaaaaata cagtgataag aatcctcttt
     4381 tattaattta ataatgccag gaatattatt gtgtataata tgcgacctca atttaagcag
     4441 cagaaaacga ctggcattaa aatcattaaa ttataaacat atacaacttt gtaaaggtgg
     4501 ttataataaa cctgattact ttaacgtctg tatacctgca ctgctgatat cacaaaacaa
     4561 taaaaatcct ggttaatatc tctgatgatt gaatgagaaa ttcacgatgt ctggtgagaa
     4621 ttctgttcaa aacgaggaat gcagacgtac atgtggcagg catggacttc taccaattta
     4681 acttttttcc ttccttgtat ttacacattg cacattttgc gctacaaaaa tttcacgtgg
     4741 cacgcaccac gtgatccgta gaaaagcctc ttgttacgaa tttcgtaaac cgcacgtctg
     4801 tctggtcgtg cggatttttt atccgtcaac ttcacatgaa acccgccaga gcactgtttc
     4861 cggattcaga catcaggcat attatcatcc acgatgtcgt gaacaagatc acacttttgc
     4921 aattccttta ttgatcgaaa gcagttaatc acggaaatta attaccggag tgaaagcatt
     4981 actgcatcgt aaatatctac catttttgac tacaccctct gacagaattt tcagagggtt
     5041 tttttatcag tttcaccatc caaccctgta cgccagaatg gtttcactgt cactcagttc
     5101 tgctattgcc tttttcatct ccctttacct tacgttctgc cagagtctga gccatttgaa
     5161 caggaggtta tcattgatat cgtgttcccg ggcaatacgg gcaacagagg ctcctggttg
     5221 caaagccagt ttaaccattt gaagtttaac tcatttgaaa atgatctgcg gggttctgcg
     5281 gataatgttt ttgctccata acaggcgccc accagtttga aaaggtgggc acctacgtta
     5341 ccaatactgg cttaatggat acatacggcg gtcggttctt acggtaaaac gacacaacca
     5401 aacgcttctt atcctgcaat gaactgcttt attaccgagt ttagtgaccg cacagacact
     5461 cacttttaag aaaaacagaa tggtaaacaa actccatgaa ttacaatagc tctctggctg
     5521 ccgccacaat agcttgcgcg gttaacccgt actctctctg taaaaagtcc tgagtttcaa
     5581 cctggccata ccgctcttta acccccaccc gacgcatggg cacaggacaa gtttctacaa
     5641 gcacttcagc aactgctgaa cctaatccat tatgaatacg gtcatgactc caattagttg
     5701 aattaggggt ataatagcca cttttaaggt tctttcatgg tcacagttaa ccttcattgc
     5761 tcctgttgcc agtccgttca ggtttaccgt cacgggaaga acccaaagga catgatcgat
     5821 ttcgctgccg tgactgtcat cgagtgcttc agctcactta ccgttacgag gcccgtaaac
     5881 cgggagttaa agagcaaatc actgaaatgg ctttcaacgg tgccggagtt cgcgatactg
     5941 cccgtacgct gaaaatcggc attaatactg tcatccggac tttaaaaaac tctcgccacg
     6001 aagaataacg tcatacccgg tcgctcatgc tgatatggct ctcatctgcg aacttgatga
     6061 gcaatggagt tttgtcggca gtaaagcgcg acagcactgg ctctggtaca cgtacaacac
     6121 gaaaactggt ggcgttctgg cctacacttt tggcctacgg actgattaaa cctgtcgtga
     6181 attgctggcg ctgcttgccc ttttaccatc ggtatgttga ccactgacga atggggtagt
     6241 tacaccagag agttaccgaa ggagaagcat ctgactggta agatttttac tcagcgcatt
     6301 gagcgcaaca tctacctctg agaacccgaa tcaaacgtct ggcccgcaga actatctgtt
     6361 tctcgcgctc tgtagaactt catgaaaaag tcatcggtgc ctttatcgaa aaatatatgt
     6421 tctactaatt ggagtcacca ccttcgtttg tttaaagaac ctgctggtcc tgccattaat
     6481 attacctgct gattgcttca catccggatt cctcgcactg cgcgcgggtt attgtttcgt
     6541 tttcggccac ggggtcgcca gggctctgtt tgcaggccat gatggcctga cctgtactgt
     6601 ttctcaggca ctgagatgtc cgtttacgcc atttgttccc ggatatcctt aacctctgac
     6661 gtttgttccc tgcccctctg tctgataacg aatacgctca caacgcacag tactgaacaa
     6721 taccgacaac aacatgtctt acatgaaatt cgctgtaaca tccaccggaa aacattttca
     6781 gatatcggat gaggtactac ttccattgac agcgcgaggt aacaaaattg atggtaactc
     6841 tgaccattaa ccgtaaaccg cagggtattt tcagtaaatc accggcaacg ccgctgcagg
     6901 ataaaacgga cacagcatac aaaatgaagg ccggtgacca gataccacag ttgaacgata
     6961 aacagaaacc tgctgataaa acaccgtggc gacatgacaa aacgccagag caaaaaccgt
     7021 aagcgcatta ttcgtcttgt ggaactctgg cctgagtcat tcaaccggga aaaaaacaaa
     7081 accacttaaa gtggggatac ctgacgacct gatacaggat atcgccatcc gagaactggc
     7141 gtttggcgct ggtacattac atgcagcagt ggcgtcttat gtgcagtctc cgcgctatta
     7201 ccgtgcgtta atagcaggtg gcgcccgtta caacctgaac ggtcagccgt gcggggaagt
     7261 gacaccacag gaacagaaag aggcagagac ccgtttaatg atgctgaatg accgacgaaa
     7321 agccagaaaa cctcgttgac tgcacaactg cgctgattca ctgagctgta atcaatgtac
     7381 agcgctgtac cggacgatgg gtaatggcga tcacaaccgg taaccccaga gacaatgcga
     7441 ctgtttttaa cgcaacatac tggccagatg tatcaccccg gacgacctga aaacgctttt
     7501 ccacccggca ttgtccgcca gcgcttatca cctgccaagt ctgttgtagt aaatccacca
     7561 gttgtgaaca ttcttcatca actgtcacac tggctcgcgg atggcagaaa agctcctggc
     7621 tgaacagcaa caggcatcgt attgttgctc ctccatgttg ttcccgcaac cagcgtaata
     7681 ccaccggcga ggatgggctg gccgtgtgat tacgctcagt aatacgttcg tgcacctgtc
     7741 ggtaaaagga agtacagaat gcatgaatct gttgcccgtt gatgtttcct tctgccgaat
     7801 agagcgtgaa gtgaaatgcc aacagatttg gccagtggtc caccgcctgc tgaacaaaac
     7861 gccggatgtc tcccggctct gaaattaagg ctttggttat ttgcacgaaa tgttcctttt
     7921 caaaccctgt tgaattttgt taagccagtg cagacacgca ggaaagcacg ccagcaccgg
     7981 tgtaatgcca gttataatca ataagaatgg aatgacgaat cagtcaggaa gagctacaac
     8041 ctgctggctg actggagaat gtctgcaaga agatgaacgt aaatgtcgcc agcaccaggg
     8101 acagggctga tttccacaat accaacgctg gtattttgca cattgccagc gtaccggcat
     8161 tacaacacgt ttcatgctgc tgcccctcgt gatgcctcga ttcgatgctg catccactgc
     8221 tccacttcac ttttgtacca gcggctgctg cgccccagtt tgatgggttt agggaaatgg
     8281 ccttcactga tcagcttgta aaaccatttg tcggtcatac cagtgaagct ggtgatgtag
     8341 ttcatgtcga tcaggggatc gtcggcacgc agcaatacgc tgtcgtgacc tgtcattgag
     8401 gtcaacatgg tcagaatgtc ctctgtgaga tttatgaatg ttgggcaaat gcgggtgaat
     8461 ctgctgagct gagctttcag agctggcctg ctacgggata ccagggatta ccggcgaggt
     8521 aacgggcttc cggatcgcgc acaccacgac gatcgcgggc gaaatcactg aacgcccata
     8581 tcagctcttt gagtctctct tcgcagtccg gtgaacaact gtccagcacc acatcccggg
     8641 tgatctgctt ctggtacggt gctgcttcac ccggtgatgg gaagcccagt acgttgctcc
     8701 aggcatccag aatcagccga tccagttcag ggccgtcgaa atactcctgt ctgacgcaca
     8761 gcaccacgtg gtattccggt ttttcaggat gacgtgtcag gagccagcag aggtcacgtc
     8821 cgggtgttgc attcatcagc gcactgacgg tttcgataaa ggccataccg aaccaggttt
     8881 ttctgtcgct gaagatgttg ccatgttccc ccggcagacg aagcaggatc cgccgtattc
     8941 gggagcagga ctgctcgcgc aacacacggt tgagaacgcc gatgattttg ttctgataag
     9001 atggccagaa gggggccagg ctatagtcga aataagggat agcattcata agatatgctc
     9061 cgttatgcgc agcccacaat gcgtcctgcc cgtgaatgag ctgacgtgtt atggtttgcg
     9121 gatggggttg atggaataag tgaagaaccg gggcagaacg ggaagatcag gtcaccgaca
     9181 agggacagaa caccggatgc aggttcagga tgaatgatct ccgttgactt cagttcacat
     9241 cattaatcaa ggcggtaagt ttttaccgac ggagtgtttc caggagcgaa ggctcaggcc
     9301 ttaagcttca gaagtgaggc ttacgaggac gccctgcagc aggacgttca ggaacttcat
     9361 tgcagccgta agcacacagc ccgtcttttt gctcttcttt cgccagataa tgcagggcac
     9421 cgcgaatatt atcgatgctt tcaggatcgt tgtggcgcac aggaatgttg atgttgtatg
     9481 tataatgcgg ctgatatgta cagcggtgtg ccgaaccaca gttatacgta attgcctgcc
     9541 agcattccgc tatccgctcc gcaaagggat atattttttt aaccctctga cgatcgatcc
     9601 agaaaaccac atgcgcatga aaaccatgat cagccgtcca ttcaatcacc cagaagaaac
     9661 caacgatatt ttcaaattgt tccacctgct ccagcagcat acgcaactgc aattcaagcc
     9721 agcgatgatc aggttgtaag aaatcaggcg tatctttcct gtagaagaaa tccatgcgta
     9781 aggcccgcag gcaggaatag cgtgccatca tttgattgat gtgattgtca agtaaagcgg
     9841 taaaaaacca gtttggtgtg taagatttag gcatgttgat gactcctgaa agttaatgat
     9901 gttttaagct ctgtgaacga attaatgctt tcgctcataa tcagatctaa aacggtaata
     9961 acttacagat ggtatttatc aggcatgctg tagcagaatg gatacgacgg gttattaacg
    10021 atacgcagca ttatgcagaa agaaatggat tatctgatgg tattagctaa agagatgaga
    10081 taaacgagga gtaaagaaaa tacagactca ataaaacatc acgaaatact gcaaaatacg
    10141 cgataaaaca ccacgtccag aaggttaaga aacatattgc gaccaatcac atcataaact
    10201 acaaacttgc tctcagtggt tgtaaaaact gtgtacaagg ccaatgcaat aaacttatat
    10261 gcgctcattc aatctgttct taaggacatg aacattcacg gctcacgata gcagaataaa
    10321 aaataccact gacatgattt tcatcctgca tcttagataa tacaacccag aacaaacata
    10381 atcaggaatc tctcctgtca attccattgt ttatccagct ctgttaatcg ttacagaatc
    10441 atggatacct tatcaaaaag taaacattcg gttaatcagt aatgacaaaa gaattgatgt
    10501 tcatcagaat agttaaaaca cttcagataa acaaccattg ttaagaaaat acagctcatg
    10561 atggataaga ttaaaattaa ttgctgtttc agtgaaaatg agaacagatg aatatcatca
    10621 gtagaatggt agagaataac ttacctgaac atcatatgag ttacagtaaa tatgattgag
    10681 ggatggaatg aatacgataa caagagtaat actaatggaa tgtgtaacga tagagtgaat
    10741 ataatcgtaa agatagaga
//
LOCUS       DAJOTB010000152        10677 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJOTB010000152 DAJOTB010000000
VERSION     DAJOTB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.152"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(91..1143)
                     /gene="fbaB"
                     /locus_tag="OHN28_003982"
     CDS             complement(91..1143)
                     /gene="fbaB"
                     /locus_tag="OHN28_003982"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCQ2299281.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEESSAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1400..2677
                     /locus_tag="OHN28_003983"
     CDS             1400..2677
                     /locus_tag="OHN28_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2299282.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2674..3678
                     /locus_tag="OHN28_003984"
     CDS             2674..3678
                     /locus_tag="OHN28_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCQ2299283.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSEIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3675..4640
                     /locus_tag="OHN28_003985"
     CDS             3675..4640
                     /locus_tag="OHN28_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2299284.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4614..5360)
                     /locus_tag="OHN28_003986"
     CDS             complement(4614..5360)
                     /locus_tag="OHN28_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2299285.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5412..6230)
                     /locus_tag="OHN28_003987"
     CDS             complement(5412..6230)
                     /locus_tag="OHN28_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCQ2299286.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(6295..7095)
                     /gene="thiD"
                     /locus_tag="OHN28_003988"
     CDS             complement(6295..7095)
                     /gene="thiD"
                     /locus_tag="OHN28_003988"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCQ2299287.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7092..7880)
                     /gene="thiM"
                     /locus_tag="OHN28_003989"
     CDS             complement(7092..7880)
                     /gene="thiM"
                     /locus_tag="OHN28_003989"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCQ2299288.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7915..8011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8103..8375)
                     /gene="rcnR"
                     /locus_tag="OHN28_003990"
     CDS             complement(8103..8375)
                     /gene="rcnR"
                     /locus_tag="OHN28_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCQ2299289.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8496..9320
                     /gene="rcnA"
                     /locus_tag="OHN28_003991"
     CDS             8496..9320
                     /gene="rcnA"
                     /locus_tag="OHN28_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCQ2299290.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9539..9877
                     /gene="rcnB"
                     /locus_tag="OHN28_003992"
     CDS             9539..9877
                     /gene="rcnB"
                     /locus_tag="OHN28_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCQ2299291.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9959..>10677)
                     /locus_tag="OHN28_003993"
     CDS             complement(9959..>10677)
                     /locus_tag="OHN28_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCQ2299292.1"
                     /translation="YSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAA
                     VQFLLCLLGGKSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESI
                     PVSQLPATGNKTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKG
                     AEDNGVGFILESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYD
                     TNKVKSGALEATALINVKYD"
BASE COUNT         2476 a         2733 c         2698 g         2770 t
ORIGIN      
        1 aagccgtaac gaagtgcgcg tgtttttatg ccggatgcaa cgtaaacgcc ttttccgtcc
       61 tacggggtgg ttctgttcgt atgaggcgca tcaggcgata gtaattttac tatcgagata
      121 aacgtcctgt acggcgttaa tcagtttcac gccgtcagcc atcgatttct tgaacgcctt
      181 acgtccaaga atcagcccca ttccgcctgc gcgtttgttg ataaccgcag tacgcactgc
      241 atcgctgagg tcagtttcac cgcctgcagc accgccggag tttatcaacc cggcccggcc
      301 catatagcag ttagctaact gataacgcac caggtcaatc gggttttcac tggtcagttt
      361 gctgtagact cgatcgtcgg tataaccgta attaatcgct ttatagccgc cgttattttc
      421 cgccattttt tgtttgacga tatctgcacc tatggttgcc gccagatggt tcgcctgacc
      481 ggtcaggtcg gcggaaacat ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa
      541 ataggcccac agcactgtca ccatgcccag ctcgtgcgca cgttcaaaag ccgcagaact
      601 ttcttcaatc tggcgacgtg actcttccga gccaaaatag atagtcgcac caaccgccac
      661 cgcgcccatg ttgaacgcct gctccacgct ggcatacagc gtttgatcgt aggtattcgg
      721 gtaacttagc gtctcgttgt gattaagttt gacgaggaat ggaatgcgat gcgcgtaacg
      781 ccgcgatacc gatgccagca cgccgtaagt tgacgccaca cagttacagc ccgcttcgat
      841 cgccagttca acaatgtttt tcgggtcaaa gtagagcggg ttagcggcaa atgaagctcc
      901 ggcagagtgc tcaacgccct ggtcaaccgg cagaatagaa agatatcctg tgccagccag
      961 acgcccggtg ttgtacaacg tctgcatatt acgtaacacc gctggcgggc gattattgtc
     1021 aatcatcacg cggtctacgt agtcatgtcc ggggagataa agctggtcag aaggaatagt
     1081 catacaacgg tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg caatatctgt
     1141 cattactatg ctcccgtaaa ttccgattgg atatcggcta tggattgtcc tggcccgcct
     1201 tttgcgggca atcataatcc tggtcgttac gggcgagttt ttccatcttt tggatgtttt
     1261 ttcagcgttt tctgttggct cgattcatca gaaaaaatgt taccgcggtc aagttttcac
     1321 cgcaaaggta tttaaaaggt attactaagt ggtattgtca tcgcgtacct tacattacct
     1381 gtcatgaagg aattaaaaga tgaaaacaac agcaaagctg tcgttcatga tgtttgttga
     1441 atggtttatc tggggcgcgt ggtttgtgcc attgtggttg tggttaagta aaagcggttt
     1501 tagtgccgga gaaattggct ggtcgtatgc ctgtaccgcc attgcggcga tcctgtcgcc
     1561 aattctggtt ggctccatca ctgaccgctt tttctcggcg cagaaagtgc tggcggtatt
     1621 gatgttcgct ggcgcggtac tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt
     1681 cttcccgtta ctgctggcct actcgctaac ctatatgccg accattgcgc tgactaacag
     1741 tatcgctttt gccaacgtgc cggatgtgga gcgtgatttc ccacgcattc gtgtgatggg
     1801 cactatcggc tggattgcct ctggtctggc atgtggattc ttgccgcaaa tgctgggtta
     1861 tgccgatatc tcaccgacta acatcccgct actgattacc gccggaagtt ctgctctgct
     1921 cggtgtgttt gcgtttttcc tgcccgacac gccgccaaaa agcaccggca aaatggatat
     1981 taaagtcatg ctcggcctgg atgcgctgat cctgctgcgc gataaaaact tcctcgtctt
     2041 tttcttctgt tcattcctgt ttgcgatgcc actggcgttc tattacatct ttgccaacgg
     2101 ttatctgacc gaagttggca tgaaaaacgc cactggctgg atgacgctcg gccagttctc
     2161 tgaaatcttc tttatgctgg cattgccgtt tttcactaaa cgctttggta tcaaaaaggt
     2221 attgttgctt ggtctggtca ccgctgcgat ccgctatggc ttctttattt acggtagtgc
     2281 ggatgaatat ttcacctacg cgttactgtt cctcggtatt ttgctgcacg gcgtaagtta
     2341 cgatttttac tacgttaccg cttacatcta tgtcgataaa aaagctcccg tgcatatgcg
     2401 taccgccgcg caggggctga tcacgctctg ctgccagggc ttcggcagtt tgctcggcta
     2461 tcgtcttggc ggtgtgatga tggaaaagat gttcgcttat caggaaccgg taaacggact
     2521 gactttcaac tgggccggga tgtggacttt cggcgcggtg atgattgcca ttatcgccgt
     2581 gctgttcatg atttttttcc gcgaatccga caacgaaatt acggctatca aggtcgatga
     2641 tcgcgatatt gcgttgacac aaggggaagt taaatgaaaa cagaacgtat tctcggtgct
     2701 ctttatgggc aggcgttagg ggatgcgatg gggatgccct ccgagctttg gccgcgcagt
     2761 cgcgtcaaag cacactttgg ctggattgac cgttttctgc ctgggccaaa ggagaataac
     2821 gcggcctgtt attttaaccg cgccgaattc accgacgata cctcgatggc gctgtgtctg
     2881 gcggatgcgt tactggaacg tgaaggcaag atcgatccgg atctgattgg gcgtaatatt
     2941 ctcggctggg cgctgcgttt cgacgccttt aacaaaaacg tactaggtcc gacctcgaag
     3001 attgcgctta acgccattcg cgacggtaaa cccgttgctg aactggaaaa taacggcgtg
     3061 accaacggcg cagcgatgcg cgtctcgcca ttaggttgtt tgcttccggc gcgcgatgtt
     3121 gattccttta ttgatgatgt ggcgctggcc tccagcccga cccataaatc cgatctggcg
     3181 gttgcggggg cggtagtcat cgcatgggcg atttctcgtg ccattgacgg agaaagctgg
     3241 tcagagattg ttgattcact gccttcaatt gcgcgacatg ctcaacaaaa acgcatcact
     3301 accttcagcg cctcactggc agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc
     3361 gatggcaccg aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagtact
     3421 attgagtccg ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaaccgt
     3481 tgcgccgtcc tgtgcgctaa ccttggcggc gacacagaca ccatcggtgc tatggcgacg
     3541 gcaatttgcg gcgcgttgca tggcgttaac gctatcgatc ctgcgttaaa ggcggaactg
     3601 gatgcagtaa atcagcttga tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa
     3661 caacgggagg cgatatgagc ggcgctcgat tacacacgct gctgcctgaa ttaaccacgc
     3721 gtcagccagt gatggtcgtc ggcgcggcgg tcattgatgt gatcgccgac gcttacgctc
     3781 tgccctggcg tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg
     3841 cactgaatat tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat gccctgccgc
     3901 tcggtcaggg cgtgtgggcg gagattattc gcaaccggat ggcgaaagag gggttaatca
     3961 gcctgatcga taacgctgaa ggcgataacg gctggtgtct ggcgctggtt gagccggatg
     4021 gcgaacgcac ttttatgtca ttcagtggtg tggaaaatca gtggaatcgc gaatggctgg
     4081 cgcgattaac cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc
     4141 cctgcggcga attgttagtg gaatggctgg aagagctgca agacgtgacg ccatttatcg
     4201 attttggccc acgtattggc gatatcccgg atgcattact ggcgcggatc atggcctgtc
     4261 gacctttagt gtcactcaat cgtcaagagg ctgagattgc cgccgaacgt tttgctttat
     4321 ccgcagagat aacaacactt ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg
     4381 ttcgtctcga taaagagggc gcatggtatt tcagcaacga cgcttctggc tgcattccgg
     4441 catttccaac gcaagttgta gacaccattg gggcgggcga cagtcatgcc ggcggcgtac
     4501 ttgccgggct ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg
     4561 cgtcgtgggt tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc
     4621 tcgcacacaa aaacgtatag atcgctgcga cagtggctga tgctgtactc aataggccgt
     4681 tgctgttggt caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga
     4741 atatgtgact gaaactcggc atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt
     4801 gggtaaatgt gctggctgcg gaagtagtca taaagagaaa tcccgatggc atcaacatca
     4861 tgaattaaat gtgccggaac ccacgattcc tcaatcgata ccgcctcttc atcgacatag
     4921 cgaatacgct taagcaaaaa aacatcactt cctgcctcga ccgccagctg ctgcgcgacg
     4981 tcttcgggac atttcacgac acgcttgtta acccatagcg tatcgggctt tttcccgcgc
     5041 aacaccacct gctgagaaaa acctcgcgct tctttcaacg agtattcgaa gatgttgttg
     5101 atctgcgtgc cgtaaccacg cgaacgcgtc accacgccct cttcttccag cgactgcatc
     5161 gccttgcgca ccgtaatgcg cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc
     5221 aaaatattgc catgctccag caccccgctg cgcacggcat tttttaccgt ttcggcaaac
     5281 ttaatataaa gcggcgtgtt atccgccgct aaaatacgct cgttcagttg ggcaatcagc
     5341 tgggtatgcg cttgttccat ttgtcttttt cctgccgggg aatctggtgg gagtatatca
     5401 ctgcttctta tttaaggcgt ttctttaatc ccatcaacga atccctgcaa ctcctcctct
     5461 gtgccattaa acacattaaa atccaccgga ccattaatgc catcgacctg tccacggtcg
     5521 gaatgctgcc agaagcgcca ggccatgccg tcattgtccg ggcgacgttg ataatagtga
     5581 gccacccacc acggatattc attgaaatag cccgccagat tggtgtgata aaaaacggct
     5641 cctgagtaaa taatcggctt ttttcccgta cgtttttcga ccatttttag ccactgactt
     5701 acccgcttgc gtaattcttt tgccgataat tttcctcgct cttctacgtc cagtaccgca
     5761 gggaaatcgc cttgtgagaa atccaccgtt tgcagaaata atcgcgcctg aactgaagcg
     5821 gatactgacg gggagaaata atgatacgcc ccgcgcagca ggccattttc ccggcttagt
     5881 tgccagttac gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc cttaataaaa
     5941 gcaaactgta agcggatacc gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc
     6001 tcctgccagc gcgaaacatc tattccgtga atggtgtaac tggcaggaat gcgaatagcg
     6061 aaagatttaa ccgggcgata accgtagaag tggatgtagt ctttaatctg aattgctgtc
     6121 gaatagaaaa aattcaccgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga
     6181 gcgcataata aaacggtgaa tttttttcga ctggtgattc tcaactgcat tggtttcatc
     6241 cctgaatgtc agcaccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca
     6301 ccaggcgtgg aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg
     6361 ggctaatgcc gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc agtttgtatg
     6421 gcgcgggcgc agtgcagcca acgccgcaga gagcgtacaa ccagtgccgt gagtgttttt
     6481 ggtcataatg cgctgtgcgg taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg
     6541 gctttgctca tcatccagat gaccaccttt cattagcact gcgccacagc ccatcgccaa
     6601 cagcgatcgc ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa
     6661 ggcggctgct tcgggcaagt ttggcgttat taatgaaacc tgtggcaata atcgactgcg
     6721 tagcgtggca actgccgaag gtgaaagcag cgggtcgccg ctttttgcca gcataacggt
     6781 gtcgagtacc acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat
     6841 atcggtttcc gccaacatac cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga
     6901 atcgagctgg gcggcgacaa aatcaggctc aatgcgatac accgactgta caccacgggt
     6961 attttgcgcc accagtgcag taataactga gcaaccataa gcgccaagtg ccgagaaggt
     7021 tttaagatcg gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc
     7081 gttaattcgt ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt
     7141 ggaacaaaac tgcctggccc ctcgcttctg gcgactgcgc gttctccggc ttgtttcatc
     7201 cagtgacagg cagatgcgat attttccagc gtatcgcctg gtagcgcaca acaggcagcg
     7261 acaaccgccg atagtgcaca gccagttcct accactttgg tcattaacgg atcgccaccg
     7321 tgaatgccaa tgatacgatg tccatcggta acataatcca cctcgccagt gaccacgacg
     7381 attgcgccag tttcccgtgc cagtgtttgt gcagcaggta tcgcgttagc tgcggcatca
     7441 gtggtatcca ctccccgtcc accattagca acgccagctg atgccatgat ttccgaagca
     7501 ttaccacgta tggcggttgg cttatgggac agaagttcca gacaaaaacg gcggcgataa
     7561 tcgagcgcac caaccgcaac cggatcaagc gtccagggcg tttgtgatcg tgttgcttgc
     7621 tcaacggcag cactcatcgc ttgcgctcgt ggccgcgtca gcgtgccaac gttaatcaac
     7681 aaggcactgg cgatagccgc aaactgactg gcctcttcgg tttcgataac catcgctggc
     7741 gatgcgccga gcgccagcaa ggtattggca gtaaaagttt gcaccacatc gttggtcatg
     7801 cagtgcacaa gaggggaatg ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa
     7861 ctcagcaggt cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt
     7921 ggtccgtgac ttccctacgc tggcattatc cagatcaggt gatacgggta tttctcagcc
     7981 ttcacaaaga agggcacccc gagtcgtttg gttgcgataa taaatcgcag aggaggatgg
     8041 taatgtccag cgcacgcgtt gtaaacgggg aaaaacggcg gtattacaat aacgttgtga
     8101 aattatttga tataagaatc cagcaccttc agaacgacat ccagatcttc ttcacgtttt
     8161 agctcatccc cctggtgaac gatgtgttcc gtcagatggc ctttaatcac ttcccgcatc
     8221 agaccgttta ccgcgccacg gatagcagca atctgttgta aaactgcagc gcattcatgc
     8281 ggttcgtcga gcattttctt gagcgccacg acctggccct gaatcttact ggcacgcgct
     8341 ttcagtttct gtttatcacg gattgtatga gacatggcaa cacctggtta acaagaatat
     8401 gaaaaatcat agcactatta atctactggg gggtagtatc aggtactggg ggggagtaga
     8461 atcagattgc cgaattaata ctaagaatta ttatcatgac cgaatttaca acacttcttc
     8521 agcaaggaaa cgcctggttc ttcatcccca gcgccatctt acttggtgcg cttcatggtc
     8581 tggaaccggg gcactcaaaa acgatgatgg cggcgtttat catcgccatc aaaggcacca
     8641 ttaaacaggc ggtgatgctc ggactggcag caactatttc gcataccgca gtggtctggt
     8701 taattgcctt tggcgggatg gtgatcagca aacgctttac tgctcaatca gcagaaccgt
     8761 ggctccagtt gatttccgca gtgatcatta ttagcaccgc gttctggatg ttctggcgta
     8821 cctggcgcgg cgaacgcaac tggctggaga atatgcacga gcatgatcat gagcatcatc
     8881 accacgatca cgaagatcac cacgaccatg gacatcatca ccatcacgaa catggcgagt
     8941 atcaggatgc ccatgcacga gcccatgcca atgacattaa acgacgcttt gatggtagag
     9001 aggtcaccaa ctggcaaatt ttgctatttg gcttaaccgg tggccttatc ccctgcccgg
     9061 cagcaattac cgtgctgttg atttgcattc agttgaaagc cctgacactg ggcacaacac
     9121 tggtcgtcag tttcagcctc ggcctggcgt taacgcttgt caccgtaggc gttggcgcag
     9181 caatcagcgt tcagcaggtc gcaaaacgct ggagcggatt taacactctc gctaaacgcg
     9241 ctccctattt ttccagtctg ttgattggct tagttggtgt gtatatgggc gtacatggct
     9301 tcatgggcat aatgcgataa ctcaatcctt taggcttgca tgttatcttc agcccaggat
     9361 gattcttaaa tcagctattt ccgctgactc tctgctcgct aaggatgctt ttagggcatc
     9421 ctttcattta cactttttac gaaatcatgg gatcactaac aaaatatcgc ttgtcagtta
     9481 tattgtatgg caggaaagat atgcgactga tattacagat ccccaaagtg gagagtttat
     9541 gaccattaaa aataagatgt tgctgggtgc gcttttgctg gttaccagtg ccgcctgggc
     9601 cgcaccagcc accgcgggtt cgaccaatac ctcgggaatt tctaagtatg agttaagcag
     9661 tttcattgct gactttaagc atttcaaacc aggtgacacc gtcccagaga tgtaccgcac
     9721 cgatgagtac aacattaagc agtggcagtt acgtaacctg cccgcgcctg atgccgggac
     9781 gcactggacc tatatgggtg gcgcgtacgt gttgatcagc gacaccgacg gtaaaatcat
     9841 taaagcctac gacggtgaga ttttttatca tcgctaaaaa aaagccccct cggagagagg
     9901 ggggaatgca gacaccttaa tatttttatt attagccact tgctcgtctt gcttgttatt
     9961 agtcgtattt cacgttgatt aatgcggttg cctccagtgc gccagattta actttgtttg
    10021 tatcgtagac gtagtaactg gccgttatcg gaattgaaac cgttgtatct gtaagttttg
    10081 actttgccgc aatttctttt aaatttgtat atcctttact gctgttagtg atatttaaaa
    10141 gtgtgactgg cgtaccatta ctttcaagaa taaatcctac gccattatct tccgcacctt
    10201 tcaggatcgt gttgctgttg gttaacaagt cagaacttga taaatacacc tgcattttcc
    10261 gtgatgtttg attttgttga cctaataaat ttttacaacg cagaataata tccccttttt
    10321 tactatttat tgttgttttg ttacctgtgg ctggtaattg tgaaacggga atactctcta
    10381 acgtaattaa taaatcgtcc ggtttgcagg ttgtggttat cgggttgtat gacaatgtca
    10441 gattaaagga atagacacct gcattttgtg ccaatgcatt tctgtagcta tttacacatg
    10501 catcccatga cttaccacct aataaacaca gtagaaattg tactgcggca ctcccacgtg
    10561 tcgatgatga agcactggat aatgtcgcaa ctccattaat catcactgaa ccaccgcccc
    10621 ccgcagaaac attcactttt gcattactgg ccgcaggtgt caccgttacc gaataac
//
LOCUS       DAJOTB010000153        10592 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJOTB010000153 DAJOTB010000000
VERSION     DAJOTB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.153"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            29..595
                     /locus_tag="OHN28_003994"
     CDS             29..595
                     /locus_tag="OHN28_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCQ2299293.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            599..1174
                     /locus_tag="OHN28_003995"
     CDS             599..1174
                     /locus_tag="OHN28_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCQ2299294.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1216..2268)
                     /locus_tag="OHN28_003996"
     CDS             complement(1216..2268)
                     /locus_tag="OHN28_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCQ2299295.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2493..3413
                     /gene="lpxL"
                     /locus_tag="OHN28_003997"
     CDS             2493..3413
                     /gene="lpxL"
                     /locus_tag="OHN28_003997"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCQ2299296.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3585..4811
                     /gene="mdtG"
                     /locus_tag="OHN28_003998"
     CDS             3585..4811
                     /gene="mdtG"
                     /locus_tag="OHN28_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCQ2299297.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            4894..5268
                     /locus_tag="OHN28_003999"
     CDS             4894..5268
                     /locus_tag="OHN28_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCQ2299298.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5269..5496)
                     /locus_tag="OHN28_004000"
     CDS             complement(5269..5496)
                     /locus_tag="OHN28_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2299299.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5668..8211)
                     /gene="mdoH"
                     /locus_tag="OHN28_004001"
     CDS             complement(5668..8211)
                     /gene="mdoH"
                     /locus_tag="OHN28_004001"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCQ2299300.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8204..9739)
                     /gene="mdoG"
                     /locus_tag="OHN28_004002"
     CDS             complement(8204..9739)
                     /gene="mdoG"
                     /locus_tag="OHN28_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCQ2299301.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10133..>10592
                     /locus_tag="OHN28_004003"
     CDS             10133..>10592
                     /locus_tag="OHN28_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCQ2299302.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLC"
BASE COUNT         2600 a         2768 c         2603 g         2621 t
ORIGIN      
        1 ctctcgcaaa ttcgttttgg atgtacttat gtcattcaca aatacccctg aacgctatgg
       61 tgttatatct gcggccttac attggctaag cgcaattatt gtctacggca tgtttgcttt
      121 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
      181 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
      241 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgcagcaaa
      301 agctggacat ctcgcattgt acctattgct gttcgccatc ggcatcagcg gctatctgat
      361 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
      421 ctccgacgct ggcgcacagg ctgactttgc cggtgccctg catttttggc tcgcgtggag
      481 cgttgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
      541 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
      601 gaaaaaaagc ctgcttggtt taaccttcgc ttccttgatg ttctctgccg gttcagcggt
      661 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgaatcca
      721 gcatcttggc tatagctggt tatatggcac cttcaaagat ttcgacggta cttttacctt
      781 tgatgaaaaa aatccagctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
      841 tactaatcac gccgaacgcg ataaacatct tcgcagtgcg gatttcctca ataccgcaaa
      901 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
      961 taccggcaat ctgacgctga atggtgtaac caagcccgtc acactggaag cgaaattaat
     1021 tggtcagggt gacgacccgt ggggcggtaa acgtgcaggc tttgaggccg aaggcaaaat
     1081 taagctcaaa gacttcaata tcaagacaga tttaggtcca gcgtctcagg aagtggatct
     1141 gattatttca gtggaaggcg tacagcagaa gtaagagtga aatgttgccg gatggcacgc
     1201 atccggcaat gatatttatt ctgttggatc aggaatgccc agtgttgtat tcagacgtcc
     1261 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
     1321 ctctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
     1381 cgcacatact ggacactgaa taaacagcag atggcagcca tcatttttac agttggtatg
     1441 gctgtcgcac ggcgcaccgc actggtggca gtgcgcgata atctcatcag atatacgttc
     1501 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
     1561 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
     1621 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
     1681 gattttttta tctttatgtg cctgcatcat ctcaactgct tttggcagct gctcacggaa
     1741 ggtatctgcc ggtatttcca gcgcgttttc aaagtgcccc acttcatact catagtggtt
     1801 acgcatgtcg ataaacaatg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
     1861 cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat
     1921 gcgatcgcgt accttcatac gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat
     1981 attcaggcgt aaaccctcta aagccggatc gaaggcatag agctgtgcgc gaaatgtttc
     2041 aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
     2101 cccaaaaaca ttcagcgcgg taaacagttg atataaagcg tcacgggtca ccttaggatc
     2161 ggcaatgtgg aaatacttat aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
     2221 tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
     2281 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
     2341 cacacatttt acgctacatt ttagcattaa aaattatttg ttatttacaa gcgcggcaat
     2401 ttcgcccagt cttcagtcac aaattttggt tgcggacgaa aaaatgcgac aatacataca
     2461 attgcccgta taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
     2521 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttggtcg
     2581 tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
     2641 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
     2701 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
     2761 tgatggaaac cggcatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag
     2821 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
     2881 gcatccattt tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggga
     2941 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
     3001 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
     3061 aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt
     3121 tcgtcccgtt gtttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
     3181 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
     3241 atcagttgat tatactgccg ccagagtgtt ctccgccgct ggatgatgcc gaaactaccg
     3301 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
     3361 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatcttcc
     3421 atgctggatg cttcagaatg gcatccggca ttaccacaga aaatccccct gatttagcga
     3481 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt
     3541 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
     3601 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
     3661 ccggtgccgc cttcagcctg gtaatgccct tcttacccct ctacgttgag cagcttggcg
     3721 ttaccggcca ctccgccctg aatatgtggt ccggtattgt cttcagcatt acatttttat
     3781 tttcggccat cgcctcaccg ttttggggtg gactcgccga tcgtaaaggc cgaaaactca
     3841 tgctattacg ctctgctctc ggcatgggca tcgtgatggt gttgatggga ctggcacaaa
     3901 atatctggca gtttttgatc ctgcgggcgc ttcttggttt acttggcgga tttgtcccca
     3961 acgctaatgc tcttatcgcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta
     4021 cgctctccac aggcggcgtt agcggcgcgt tgctcggccc aatggctggc ggtttgctcg
     4081 ccgatagcta cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct
     4141 ttttcgtcac cctgttttgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc
     4201 tgcacatgcg ggaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg
     4261 tcaccacgtt aatcatccag gtggcgacgg gctcaattgc ccccattctg acgctgtatg
     4321 tccgcgaact ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg
     4381 tgccaggcgt ggcggctctg ctaagtgcac cacgactcgg caaacttggc gatcgaatcg
     4441 gacccgaaaa gatcctgatt acggcgctga tcttttctgt actgctgttg atcccaatgt
     4501 cttacgttca gacgccattg caacttggga ttttacgttt tttgctcggt gccgccgatg
     4561 gtgcactact ccccgccgta cagacactat tagtttataa ctcgagcaac cagatcgccg
     4621 ggcgtatctt cagctataac caatcgtttc gtgatattgg caacgttacc ggaccattga
     4681 tgggagcagc gatttcagcg aactacggtt ttagggcggt atttctcgtc accgctggcg
     4741 tagtgttatt caacgcagtc tattcatgga acagtctacg tcgtcgtcga atcccccaga
     4801 tatcgaactg atttttcgcc tttcatactt gcaaaagcgg agaatcagct atccttttcc
     4861 ctgaaacctc atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa
     4921 gccattgacg ctgcacgcga agaatttctt gcagacaacc ccggcatcga cgccgaaaac
     4981 gcgaatgtgc aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg
     5041 caagttgagt tttttgccga cgaaggggaa gaaggtgaat gtttacccat gcttagcggt
     5101 gaagctgcgc aaagtgtctt tgacggcgac tatgatgaga tagagatccg ccaggagtgg
     5161 caagatgaga atacattaca tgaatgggac gagggggaat ttcagcttga gccaccgctg
     5221 gataccgagg aaggacgcac agcagctgat gagtgggatg aacgttaatc actcatacgg
     5281 gccatgatga atgtcgatcg gcagcagtaa ggtatcgaaa accagggaga atggcagatc
     5341 aaggatcgtg acgtaacgcc aggcggagtc acgcacgtcc cattgcacac cgggataata
     5401 ttggttgcca tgcccctgcc ccggtatagt gcgactaatg atgctgccac agccactcag
     5461 caaggtcacc attatgctca ccacaattaa acgcatttga tacctcttcc gtaaaaaata
     5521 ccggcataac gccggtattt ttattggctc gttgtttttg ccggatgcgg cgtgaacgcc
     5581 ttgccagacc cacaaaatct tgcaaaatca ataaattgca ggaacgatgt aggcctgata
     5641 agcgtagcgc atcaggcaac tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc
     5701 cagtggattg agctttatcc cttcgtaata actcacccat gaagaatatc tctccgggga
     5761 attccagacg cggaaatgca gacgggccat cgtcaccgga tcgcttagca gcaccaggcg
     5821 acgatcgcga ttcagcttct ctggcgtctc gttcagcgcc tgttcaacgt ggcggtcacg
     5881 ggcgatttcc agcaccttgc tggcgcggtg acgcgcggtc gccattgcgg ttgccagagc
     5941 gttaaatgac gggttaaaca ccgcgtgcat aaaaccatca tcaagggagc gttgacgatt
     6001 catctcaagg aaccgatcgg tatcaaccag cacctgcggc ggtgaatact cttccgggat
     6061 caggaacagt ttccagcgtt tggttcgcag accaacggtg gcacggctgg aaatcaccga
     6121 aacaaacggt gacaggatca acgagaagac aatcggtgcc agccagaaca gaaaacgcag
     6181 atccagccac gccatcccaa cagcccacac taaccctaac agcagctgtg agccgtggcg
     6241 tttgaacgct tccccccagg aagtggagtc atcatcacgc tgcggcgaat tccacactac
     6301 ttcccagcca aggaacgcgc tgacgacgaa gaccgtatgg aacagcatgc gtaccggagc
     6361 cagcagcacg gaaaacagca cttccagcag caacgataat gtaacgcgcc agaagccgcc
     6421 gtattctttc gttcctttgc accagataag caaaatgctc aatagcttcg gcaggaacaa
     6481 cagcaccatg gtcgaagcaa aaagtgcaat cgccagctca ggacgccact gcggccatac
     6541 cgggaacaac tgccgtggtt gcaggaagta ttgcggttcg gtcaacgcat gtactacctg
     6601 caatgcagta gagagcgcga ggaacataaa ccacagcgga gcggagagat aagacatcac
     6661 gcccgtcagg aacaccgcac ggtgaaccgg gtgcataccc ttcaccagga acagacggaa
     6721 gttcatcagg ttaccgtggc accagcggcg gtcacgtttt agctcatcaa gcaagttagg
     6781 cggcaattct tcataagaac ccgggagatc gtaagcaatc cagacccccc aacctgcacg
     6841 gcgcatcaac gccgcttcca cgaagtcatg tgacaggatt gaaccggcaa aagaaccttc
     6901 gcccggcagc ggagccagtg cacagtgctc gataaacggt ttcacgcgga taatcgcgtt
     6961 atgcccccag tagtgcgact cgccaagttg ccagaagtgc aaaccggctg taaacagtgg
     7021 cccatacacg cgggtcgcga actgctgaca gcgcgcatac agcgtatcca tgccggacgc
     7081 tttcggcgac gactgaatga tcccggcgtt cgggttggct tccatcaggc gcaccaggcc
     7141 gcacaaacaa tcaccggtca ttaccgagtc agcatccagc accaccatgt agctgtactg
     7201 gctgccccag cgacggcaga agtcatcgat attaccgctt ttacgcttca cgcgacggcg
     7261 gcggcggcga tagaaaatct gaccttcgcc accgacttca gcgataagct ccatccaggc
     7321 tttttgctct gcgacgcaga tatccgggtt ataactgtca ctcagaatgt agacatcaaa
     7381 gtgtttggca ttcccggtgg cttttactga ttcccacgtt gcacgcaagc cagcaaaaac
     7441 acggttcacg tcttcgttac agataggcat gatcaacgcc gtgcgatgct ccgggtttaa
     7501 tggttcatcg ccaactgttg acgcagatat actgtattta tcgcgaccaa taagcagttg
     7561 caggaagccc atcaacgccg tccagaaccc ggcggacacc caacagaaca gtaccgcaaa
     7621 gaggatcagg ataccggttt gcagcatata aggcagaagc tgcataaagg aaacccacac
     7681 atcctgacca accatatcca taggattaat cagcgcccac ccctgataag gaagaatggt
     7741 cttcatatac caggtcgcga caacagtttg cgcgagcgtc aggatcaaca gaatgtaacg
     7801 gcggatggta ccgacggtac gccacttttg ctcactctcc tgctcttctt tggtcaaacg
     7861 agccagatag cgcggcgtca catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg
     7921 gttggtacgc cacgggtcgg gaaacatcga ggagcgtttt gcttctggca tcgcctttag
     7981 ctgatcgcgc ccttcgtcgt ctttaattaa ctgtccatca gcaagtgaat ctggccaggc
     8041 ttgttccaga cgcgccttta ccgagccttg cggggagtca tcctcccgcg cccaggtgcg
     8101 gtgttcggca tccagcgcct gatgaacggc gcggatatca gtcttcggca atgccgcttt
     8161 ctcgcttgcg gcgatgggca ttgcgtcaat gtactcagtt gtcttattca ttggcaggta
     8221 actggtagct ccaggtttca ctcaacgtct gatcggcatt caccagcgca gcacgcattt
     8281 cagtggtttt cttggcatct ttcactttca cacgcatcac cagacgccaa cctttggtaa
     8341 ccgggttata gcgcaccgtg ctttcaacta tctcaccatt atcaccaatg ctggtttgcg
     8401 ctgtgaccgg ggtatcctct ggcagttttt tcatctcagc gccggtaaaa tcgaccacaa
     8461 aggcgatagt accgtcaggc tggcgaatca ggttcgactg cttcacatcc cccgttgaac
     8521 gacgcgtttg ttgcacccat gcgttatctg gcgcatgcag tttgtcttca tcacggctga
     8581 aggtgatggt gtatttaaag ttcatctctt tacccggctc cggcagctga tccggcgtcc
     8641 agtaagcgac gatgttatcg ttggtttcat cgttagttgg aatttccacc agctcaacgc
     8701 tgcctttgcc ccactcccct ttcggagtca cccatgcgct tggacgaaga tcgtaacgat
     8761 catcgagatc ttcaaagcgg gagaaatcac gaccgcgctg caacagaccg aagccttgcg
     8821 ggttttccat cgagaagctg ctgacagcta aatgtttcgg gttattcaac ggacgccaga
     8881 tccattcgcc attaccagca tggatagaca gaccgttaga gtcgtgcaac tccggacgat
     8941 agttatttgc aggcgacggt tggttcggcc caaacaggaa catactggtt aacggtgcaa
     9001 cccccagttt gccgacttta tcgcgcagat agattttcga ctgcacatcc acaaccgtgt
     9061 cacgtcctgg cataactacg aatttgtaag cacctgtcgc gcgcggcgag tcaagcaatg
     9121 cataaatggt taaacgttta tcagtcggtt ttggacgctc gatccagaac tctttgaagc
     9181 gtggaaattc ttcacccgat ggcaaggcgg tatcaattgc caggccgcgt gcagaaaggc
     9241 cataaacctg acctgcacca atcacgcgga aatagctggc cccgagcatg ctgacgattt
     9301 catcgttttt atctttgctg ttgatcgggt aaagcacttt gaaaccggca aaaccaaggt
     9361 cttttaccgt gtctttgtca tgctgaacat cgccgaaagt gaaataatcc gggctgtatt
     9421 tgattcgttt gactgcggtg gcagtcactt catttatttt gaccggggta tcgaagtaca
     9481 taccctgatg gtagaactcg agtttgaatg gggtcttcag attgttccag tacgctttgt
     9541 catgattaaa ctggatctgc tgatagtccg cgtatttcat atcgcggaaa acggagggca
     9601 agttgctttt gggcgcctca tagcctttcc cggctaagga ttgagcttgc tttgcgacat
     9661 catcaatact gaaagcccag cttgaagatg tatacagggt taacattact gcagcactca
     9721 accaacgcat tttcatcatt tgtagtttat gtttcataat tagtaagcac ttcccccttt
     9781 gtgtgcttat atcgatccga tctattttaa tggataacct gccattccga caacagaatc
     9841 ccggtatcgt tcagcacact ggctcatgga ttaagcacct gattgcaacc gttttcactt
     9901 tccgtgcttc tcctaaagac agaagcatcg acttcgtgta gggtttgcca gcaaatttat
     9961 ttatcaaccg tcttacagac gcttccattc tggttagtgg tagcgatgat gctatgcata
    10021 aaaaccttga agcctgcgcc tggcgcggtc aggtcggtgt cactatggca acaagcctaa
    10081 aggcataacc cgacagggat aagacgaaaa atctgagatc agttaaccat atatgaatcc
    10141 agtacccgcg caacgtgaat atttcctcga ctccatccgc gcctggctga tgttgttagg
    10201 gatccctttt catatttctt taatctattc gagccataca tggcatgtga atagcgccga
    10261 gccgtcattg tggctgaccc tttttaatga cttcatccac tcgttccgca tgcaggtgtt
    10321 tttcgttata tccgggtact tttcctacat gcttttttta cgctatccct tgaaaaaatg
    10381 gtggaaagta cgtgtcgaac gtgtaggtat cccgatgtta acagccatcc ccttactgac
    10441 attgccgcaa tttattatgc tgcaatacgt caaaggcaaa gcggaaagtt ggcctgggct
    10501 gtcattgtat gacaaatata atacgttggc ctgggaatta atatcacacc tgtggttttt
    10561 actggtgtta gtggtcatga cgacgctgtg cg
//
LOCUS       DAJOTB010000154        10557 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJOTB010000154 DAJOTB010000000
VERSION     DAJOTB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.154"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..1048
                     /locus_tag="OHN28_004004"
     CDS             140..1048
                     /locus_tag="OHN28_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCQ2299303.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1255..3246)
                     /gene="tkt"
                     /locus_tag="OHN28_004005"
     CDS             complement(1255..3246)
                     /gene="tkt"
                     /locus_tag="OHN28_004005"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2299304.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3524..4282
                     /gene="loiP"
                     /locus_tag="OHN28_004006"
                     /gene_synonym="yggG"
     CDS             3524..4282
                     /gene="loiP"
                     /locus_tag="OHN28_004006"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCQ2299305.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4486..5406)
                     /gene="speB"
                     /locus_tag="OHN28_004007"
     CDS             complement(4486..5406)
                     /gene="speB"
                     /locus_tag="OHN28_004007"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCQ2299306.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5542..6273)
                     /locus_tag="OHN28_004008"
     CDS             complement(5542..6273)
                     /locus_tag="OHN28_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299307.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNELRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAK
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6419..8395)
                     /gene="speA"
                     /locus_tag="OHN28_004009"
     CDS             complement(6419..8395)
                     /gene="speA"
                     /locus_tag="OHN28_004009"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCQ2299308.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8404..8535)
                     /gene="yqgB"
                     /locus_tag="OHN28_004010"
     CDS             complement(8404..8535)
                     /gene="yqgB"
                     /locus_tag="OHN28_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCQ2299309.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8671..8886
                     /locus_tag="OHN28_004011"
     CDS             8671..8886
                     /locus_tag="OHN28_004011"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299310.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            9190..10344
                     /gene="metK"
                     /locus_tag="OHN28_004012"
     CDS             9190..10344
                     /gene="metK"
                     /locus_tag="OHN28_004012"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCQ2299311.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2619 a         2826 c         2669 g         2443 t
ORIGIN      
        1 attgaatgtc cgaaagcata cttttctaat gcaatgtttt ttcgctatac ccctttatag
       61 attctgaatt ctcacctgcc agctgttttc tcctcccccc atgtgacata ttcccatact
      121 ttactcaagg aagattcata tgaaagcacc tgtttcaata acctcatttc gcccacaaaa
      181 atctattgct atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta
      241 caccgttaat gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc
      301 cgagccgggg ctgggtttta agatcccttt tatggaaagt gtagagaaaa tttccacgcg
      361 taatcaggct gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca
      421 aatgacggta tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac
      481 ctacaacacc attgaagcat taaaagatcg tctgattgtc cgtcagttgc caactcaact
      541 ggagaacgtt ttcggccaat ataccgccat cagcgcggta caggatcgta caaaactggt
      601 tcaggatctg caaaatgcaa tgcgtaaagc cgttgttggc ccggtggtga ttgatggtgt
      661 ccagattgaa aatattgatt tttctgacgc ttatgaaaag tcgattgaag accgaatgaa
      721 agcagaagtc gccattgcca cacgtaagca aaatctggaa acagagaaaa tccaagcgca
      781 aatagcagta actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga
      841 agctgagacc attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc
      901 ggctgaagca gaagctatcc gcctgcgcgg tgaagctctg cgggagaatc ccggacttgt
      961 tgccctcacc actgctgagc gttgggacgg taaactgccc gataccatga tccccggcag
     1021 cacagttccg tttatctcta ctaagtaaaa aaaggccgct ttcgcggccc ttgagactga
     1081 tagcaaagac aaaactgcct gatgcgctac gcttatcagg ctgcaatatg ttgaatttgc
     1141 aaaatcttgt aggacggata aggcgttcac gccgcatccg gcataaacaa cgcgcacttt
     1201 gccaacaatc tgaagggccg catttgcggc ccttctcaca aagcatctta ccgattacag
     1261 cagcgctttt gctttcgcaa caacgttgtc aacggtgaag ccgaactctt caaacagctg
     1321 ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcaccgt tcaggccaac
     1381 atacttgtac cagtagtcag caatgccagc ttccaccgca acacgagcgg taaccgcttt
     1441 cggcagtacg gattcacggt aagccgcgtc ctgcttgtcg aatgcgtcgg tagacggcat
     1501 ggaaaccacg cgcgctttca cgccttcggc agtcaatttt tcgtatgccg ctacagccag
     1561 ttcaacttca gaaccggtcg cgatgaagat cagttccggc tgaccagcac agtctttcag
     1621 cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct gctgcgccag
     1681 gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgttcaacgc cgtatttcca
     1741 cgctaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg gggtcacgcg
     1801 cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca gaccgatgga
     1861 gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac gtacggcgtt
     1921 acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaaac caccgtgcag
     1981 ggagataccg ttggcaatcg cagtcatacc gaattcacgt acaccgtagt ggatgtagtt
     2041 acccgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt tagacggcgc
     2101 caggtctgca gaaccgccga ggaattccgg caacagcggc ccgaacgctt cgatagcatt
     2161 ctgagacgct ttacggctgg cgatcttcgc cgggttagcc tgcagtttag caatgaactc
     2221 tttcgctttc gcgtcgaagt cagacggcat ttcgcctttc atacggcggg taaattcagc
     2281 ggcttcctgc ggataagctt tcgcgtaagc agcgaatttc tcgttccatg cggactcttt
     2341 cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga tttcgaacgg
     2401 cgcgtatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc
     2461 accgtgggag tcgtgggtac cggctttgtt cggggaaccg aaaccgatga tggttttgca
     2521 catcagcagg gacggtttgt cagtcactgc gcgcgcttct tctactgcgc gtttgatgga
     2581 tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat
     2641 tgcggtgtcg tcggtaaacc agccttcaac gtgaccatcg atagaaatac cgttgtcatc
     2701 gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa cttcgtggga
     2761 gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt cgacgatgtc
     2821 gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca taccgactgc
     2881 gttggcaata ccctgaccca gcggaccggt ggtggtttct acacctgcgg tgtaacccac
     2941 ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt cttccatcgg
     3001 cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt ggccgttgga
     3061 cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt tcaggaaatc
     3121 acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt gaccggattt
     3181 ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct ctttacgtga
     3241 ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt gacgacagcg
     3301 cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc gtcatgtaca
     3361 tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt gcgcaatcta
     3421 ctcgccggtc cactgcactt ttccttatac ttcgattgag cgtcaattca cctgcagatg
     3481 gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt
     3541 attggtagca atgagcgtgg caacggtact gactggttgc cagaatatgg actccagtgg
     3601 actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa
     3661 aaccctgagc gatcaggcat gtcaggatat ggacagcaag gcgacgattg cgccagccaa
     3721 tagcgaatac gctaaacgtc tgacgacaat ttctcgcgcg ttaggcgata acatcaatgg
     3781 tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg caatggctaa
     3841 cggctgtatc cgcgtctata gcgggctgat ggatatgatg acggataacg aagtcgaagc
     3901 ggtgctcggt cacgaaatgg ggcacgtggc attaggccat gtgaagaaag gaatgcaggt
     3961 agcacttggt acaaacgcaa ttcgcgttgc ggcggcctct gcaggcggaa ttgtcggcag
     4021 tttgtcacaa tcacaactag gagacctggg cgaaaagcta gtcaattcgc aatttaccca
     4081 acgtcaggaa tcagaggccg atgattactc ctacgatttg ctgcgccaac gcggtatcag
     4141 cccggcaggt cttgccacca gctttgaaaa actggcgaaa ctggaagaag gccgccaaag
     4201 ctcaatgttt gacgaccatc ctgcatccgc cgaacgcgcc cagcatattc gcgatcgtat
     4261 gagcgcggat ggagttaagt aaagccaggt agtgccgaac gtaggtcgga taaggcgttc
     4321 acgccgcatc tgacaagaat ggcacatttg ttactccgtg cgcattgccg gatgcgatgc
     4381 tggcgtatct tatccggcct acgggtgccg agtgtaggtc ggataaggcg ttcacgccgc
     4441 atccgacatt aatggcacat cacccgcgcg cctcttatct ggcgcttact cgcccttttt
     4501 cgccgcctgg atatacagca tctccagcgc cagcgtcgcc gcagccagag cggtgatttc
     4561 cgactgatcg tatgccggag ccacctccac tacgtccatc ccaacgatgt tgagatcttt
     4621 caagccgcgt accagtttaa tggcgcgatc ggaggtcagg ccgccaatta ccggcgtacc
     4681 ggtgcctggt gcaaaagcag gatccaggca gtcgatatca aaggtcaggt aaaccggcat
     4741 atcacccaca atctgtttca cctgggcgat aatgtcatcc acgctgcgat cgttcacctg
     4801 gcaggcgtcc agcacggtaa agccgttgtc tttatcaaac tcggtacgaa taccaatctg
     4861 cacagaatga ttcgggtcga tcagaccttc tttcggcgcg gtatagaaca tggtgccgtg
     4921 gtcaaattca caaccgttag cgtaggtatc ggtgtgggca tcaaagtgta ccaacgccat
     4981 tttgccgaaa tgcttcgcat gagcgcgcag cagcggcagc gtaacaaagt ggtcaccacc
     5041 gaaggagagc atacgcttac cggcagccag cagcttctcg gcgtgcgcct gcagcttctc
     5101 gctcatctca cgggcatctc cgaaggcata taccagatcg ccgcagtcca cgacgttcag
     5161 gcgctcacgc atgtcgaaat tccacgggaa gcgattgtgt tcccaggcca gattcgtcga
     5221 aacctgacgg atcgctgccg gaccgtggcg accacccgca cgaccagaag tggccatatc
     5281 gaacggcacg ccagtaatca cccagtcagc atcgctgtca tatggctgga agttcatcgg
     5341 caggcgtaaa aaaccaaagg cgttggaaac cagtgagtta tcgtattgat gacctaaggt
     5401 gctcatggat ggactcctat tacaaagtcg atataaaaaa acccttccgc gtcgttaggc
     5461 ccgacgagga agggttggat ttgtgaaaca caagtttgtg ctaattatcg ccttaaatgg
     5521 gcgaggcatc aagcgaggtt gctatttccg acacggtggc ggcgcatagc cattgttatc
     5581 tgccggaaag gcttgtccag agcacggcgg cagcgcgttt ttcgccgggc cttgtaattc
     5641 aaaggcggca aagccgatta caccggtatt acgcacatcg ccttgcgccg aattggctgc
     5701 gtaggaatca gcaacgttgg cgaactgaaa agccgcttcg gtatgtttat ctttgcggaa
     5761 tcctttaatc gctaacgaat ctccggcatt cacaatataa ccgttgttat tcagcgaacc
     5821 ttgcttgccg ttcagcacat ccaggccatc aaccgtagca acgatctcgt agttggtgtt
     5881 acgactgtaa ttacggacat ataattgata attcattccg actctggcat ggacaatata
     5941 gcgtagttca ttattggatg aatcataaac ccgcgtaata ggtagagaat taaaattacc
     6001 atcgcgaata gcatattcca gttcgttaat acgaatgcta taaacgcggt cgtagccgga
     6061 aggaacatta gtcgaataac taataacgat catctctgcc ggatcgcgcc aggctcgttc
     6121 agcagatacg ccctgaaccg ttgagtgcac atccccaccc caggcggtgc cgacattgcc
     6181 tgatgcactg ctattatact gcgcattact ggaacaacca gccagcagca cgattaatgc
     6241 caccaacgcg ctacgtactt tccatttttt cataaccgtt ccctctgcat ttaattaatg
     6301 cagcgattgt agggagatta tttaataaga aaagcagcta aattattttt aagaatatat
     6361 aaaaagaaag cgggccgatt tacgtaaata aatcgccccg ccagcagaat aatcttaatt
     6421 attcatcttc aagataggta taaccgtaca aacctgcctc gaactcttcc aggaactgtt
     6481 gttgcagttc agcatcaaga tcggttttct tcacctgatc gcggaactga gttaacagcg
     6541 ttttcggatc gagctgcaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6601 gttctacctc tacgctacca tcagggaaga cgaacacgtc aaccgcttcg gtatcaccga
     6661 acaggttgtg catgttgccg aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6721 tcggcggatt ctccggatcg tactccggca taggcattgt cgtggcaata ccgtcaccat
     6781 caatatagtg gtcgatagca ccgtctgagt cacaggtaat atccagcagc acagcgcggc
     6841 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6901 acgcatccgg catcgactgg aacagcgaga agttgacgta cattttgtcc gccatacgtt
     6961 cctgcagctc gtcgataatc ggacgatgcg cacggttttg cggatccagc tgcttttgca
     7021 cttcatggca catgctcaaa taaagctgct cagcccatgc gcgttcttgc aggctaaagg
     7081 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     7141 cacgcagaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     7201 gcagggcgcg cggtgcatct tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca
     7261 cgccgatgat attcgacacc agcacggtgt gatgcgcagt caccgcacga cccgattcgg
     7321 tgattaccgt cggatgtggc agaccatttt cttcacacgc atcaccaatc gcccagatga
     7381 tgttgttggc gtattcattg aggccgtagt ttacggagca gtcagactga gaacgggtgc
     7441 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7501 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc tgtcgcgata tcgcgaatat
     7561 tcgccatctg cgaaccgagg tggaagtgca gcagttgcag gctgtcgaga cgcccggctt
     7621 cacgcagcgt ttcaaccagt tgcagtactt gagtcgccgc caggccaaac ttcgattttt
     7681 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7741 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcaatt tctgacatct
     7801 tctcaatgac cagatagacc ttgtggccca tcttctcgcc aattaatgcc aggcggatat
     7861 attcgcggtc cttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7921 gcaccgccat caactcggct ttggaaccgg cttccaggcc cagcggttcg cccgaatgaa
     7981 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaacg aggaagtaat
     8041 cgccgttata gccgtaggat tcccgcgctc gtttgaacgc ggcgttaatg gaacgcaaac
     8101 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcgc
     8161 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     8221 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     8281 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     8341 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     8401 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8461 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8521 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8581 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtagctgta
     8641 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8701 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8761 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8821 aaaactctgt tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8881 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8941 cgcagaatgc cagcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     9001 gattcaaaaa aaagtggaaa ttgggtgaag aactgaccta aaatagccat ccagatgtta
     9061 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     9121 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     9181 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     9241 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     9301 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     9361 accaccagcg cctgggtaga catcgaagag atcacccgta ataccgttcg cgaaattggc
     9421 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgccatcggt
     9481 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9541 ggcgaccagg ggctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9601 cctatcactt atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9661 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9721 atcgttggca ttgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9781 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9841 acttctgcca ccaaattctt catcaacccg accggtcgtt tcgttatcgg tggcccgatg
     9901 ggtgactgcg gtctgactgg tcgtaaaatc atcgttgata cctacggcgg catggcgcgt
     9961 catggtggcg gtgcgttctc cggtaaagat ccatcaaaag tggaccgttc cgcagcctac
    10021 gcagcacgtt atgtcgcgaa aaacattgtt gctgctggcc tggccgatcg ttgtgaaatt
    10081 caggtttcct acgcaatcgg cgtggctgaa ccgacttcca tcatggtaga aactttcggt
    10141 actgagaaag tgccttctga acaactgact ctgctggtac gtgaattctt cgacctgcgc
    10201 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
    10261 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
    10321 cgtgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
    10381 tggccttttt cttttacctc accgcagtgg acaggcgttc acgccgcatc cggcaaaaaa
    10441 ccgcccgcac aataacatca ttcttcctca acacgtttta ccgcaaatta tcatcactac
    10501 tgaaatcgat tacaccaacc acaccagcca aatatttgta atattttcat attatta
//
LOCUS       DAJOTB010000155        10412 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJOTB010000155 DAJOTB010000000
VERSION     DAJOTB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.155"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            317..1087
                     /locus_tag="OHN28_004013"
     CDS             317..1087
                     /locus_tag="OHN28_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCQ2299312.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1078..1551
                     /locus_tag="OHN28_004014"
     CDS             1078..1551
                     /locus_tag="OHN28_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCQ2299313.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1659..2198
                     /gene="dnaT"
                     /locus_tag="OHN28_004015"
     CDS             1659..2198
                     /gene="dnaT"
                     /locus_tag="OHN28_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCQ2299314.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2201..2938
                     /gene="dnaC"
                     /locus_tag="OHN28_004016"
     CDS             2201..2938
                     /gene="dnaC"
                     /locus_tag="OHN28_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCQ2299315.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2987..3481
                     /locus_tag="OHN28_004017"
     CDS             2987..3481
                     /locus_tag="OHN28_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCQ2299316.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3735..6026
                     /gene="opgB"
                     /locus_tag="OHN28_004018"
     CDS             3735..6026
                     /gene="opgB"
                     /locus_tag="OHN28_004018"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCQ2299317.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFVALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSRTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGAEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRIGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6163..7185)
                     /locus_tag="OHN28_004019"
     CDS             complement(6163..7185)
                     /locus_tag="OHN28_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCQ2299318.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEVLIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGLLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7324..8229
                     /locus_tag="OHN28_004020"
     CDS             7324..8229
                     /locus_tag="OHN28_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2299319.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8400..10052)
                     /locus_tag="OHN28_004021"
     CDS             complement(8400..10052)
                     /locus_tag="OHN28_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCQ2299320.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLELPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2760 a         2655 c         2418 g         2579 t
ORIGIN      
        1 tgcgggaatc acacaatcaa tatttattca ataaaatctg atggttttaa cttgttaaca
       61 ttaaaaaacg cgcccagcta tctattatcc tgactcgtaa aggccaaata acaaattcat
      121 tatagtgata atattcatgc gatagtttgt ttacggcgag agataattcg ctttttatca
      181 ccgagcatct cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa
      241 acaaccgtgc ttcatgatac aaatggattc tcattatctg aacaatacgc agcacgtcta
      301 tgacaaaggg cgagttatgc aaactgagca acagcgagcc gtaacacggc tttgtatcca
      361 gtgtggatta tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc
      421 acgactgggt cgggcgttgg gaatggacag cgtcgaaagt tctatctctt cgaacgccat
      481 agtgctgaca accattaaag atgggcaatg cctgacatcg acacgtaaaa atcacgatcg
      541 cggcattaat atgcatgtgg tgactgaagt ccagcacatt gtaattctgg cggagcatca
      601 tctgctggat tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc
      661 aagatggctg gtagccttaa tggttggcct ttcctgcgcc tgtttctgta aactcaataa
      721 aggtggctgg gatggtgccg tcatcacctt cctggccagt acggccgcga tgtatatccg
      781 ccagctactg gcacaacgtc atcttcatcc acagatcaac ttttgcctca ccgctttcgc
      841 cgccaccacc atttccggat tgcttttgca acttcccact ttcagcaata cccccaccat
      901 tgcgatggcc gccagcgttc tgctgctggt gccgggcttt ccgttgataa atgctgtcgc
      961 cgatatgttt aaaggccaca tcaataccgg actggcacgc tgggcaatcg ccagtctgct
     1021 gacactggct acctgtgtcg gcgtagtaat ggcactgacg atttgggggc tacgcggatg
     1081 ggtgtgatcg aatttctgtt agcgttggcg caggatatga tcctcgccgc cattcctgcg
     1141 gtcggctttg cgatggtgtt caacgttccc gttcgagcgt tacgctggtg tgcgctgctt
     1201 ggtgcgatag gtcatggttc ccgaatgatc ttaatgacca gcgggctgaa tattgaatgg
     1261 tcaaccttta tggcctctat gctggtcggc accattggta ttcaatggtc gcgctggtat
     1321 ctggcgcatc cgaaagtgtt taccgtggcg gctgttatcc ctatgttccc tggcatatca
     1381 gcttataccg caatgatttc ggcggtaaaa atcagccagc taggttacag cgaaccgttg
     1441 atgattaccc tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc
     1501 ggtctttcca ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc
     1561 acttaaagca cgcggatgag agacagcctc ctctcctccg tgtgttacta taaaagttat
     1621 ctcccttctc gttcatcgtt ccatatttga gaaacagtat gtcttccaga gttttgaccc
     1681 cggacgtcgt tggtattgac gccctggtac acgatcacca aaccgttctg gcaaaagctg
     1741 aaggcggtgt ggttgccgta tttgctaaca atgccccggc gttttatgcc atcactcctg
     1801 cacgcctggc tgaactgctg gcgctggaag aaaagctggc gcgtccggga agtgacgtgg
     1861 ccctggacga tcaactctat caggaaccgc aaaccgctcc cgttgccgta cccatgggga
     1921 aatttgccat gtatccggac tggcaacccg atgccgactt tatccgcctg gcggcgctat
     1981 ggggcgtggc actaagagag ccggtgaccg ccgaagaact ggcgtcattc attgcctact
     2041 ggcaggcgga aggcaaagtc tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc
     2101 tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg agatgtgaat acggtcagtg
     2161 aacctgacag ccaaattcca ccaggattca gagggtaacg atgaaaaacg ttggcgacct
     2221 gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa cctgctttca aaacgggtga
     2281 agaactactg gcctggcaga aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga
     2341 aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc ggtattcgtc cactgcatca
     2401 gaactgctcc tttgagaact atcgcgttga gtgtgaaggg caaatgaatg cgttaagcaa
     2461 agcgcgccag tatgtcgaag agtttgacgg caacatcgcc agcttcatct tttctggtaa
     2521 gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc tgctacgcgg
     2581 taaatccgta ttgatcatca ccgtggccga tattatgtcg gcgatgaaag ataccttcag
     2641 gaatagcggt accagcgaag aacaactgct taacgatctg agtaacgttg atctgctggt
     2701 gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca tcaaccagat
     2761 cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga
     2821 agagatgacc aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt
     2881 gtgggtgatc ttcaactggg atagctaccg tagccgtgta acaggtaaag agtattaatc
     2941 cccgtttctg gcggtatact gcgacacaat tcaaccttga gtaatgatga tgaaaactat
     3001 taaacatctt ctgtgctgtg ccattgccgc cagcgcatta atttccaccg gggtgcatgc
     3061 tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc aaaacaacac
     3121 gacacaggaa ggcggttggt cgctcgcgtc attaactaac ttgcttagca gcgggaacca
     3181 ggccttaagc gcagataaca tgaacaacgc cgcaggcatt ctgcaatact gcgcgaagca
     3241 aaagctggct tcggtaaccg atgccgaaaa catcaagaat caggtactgg aaaaactggg
     3301 cctgaacagt gaagagcaaa aagaagacac caactatctg gatggcattc agggtttgct
     3361 gaaaaccaaa gatggtcagc aactcaatct ggataatatt ggaacgactc cgctggcaga
     3421 aaaggtgaaa accaaagcct gcgatctggt gttaaagcag gggctgaact tcatttcctg
     3481 aaccgcgaaa cgccgcgttt tagctgttac ccggctgacg cggctctctc ctgccctttt
     3541 ccctgctcct ggacggtttt acccctgtta cacgggaagc gatcgcgatc aaaaaagtct
     3601 gtttataaac taattcctaa caacatcgca tttttttgac actagaattg cagcaatatg
     3661 gcgacgccat tacattgtgc ttctcaaaaa gcttaaacgt tggctaaacg gcccggttta
     3721 gtgaggatcg cccgttgtca gaactactct ctttcgccct ttttctcgcc tctgtgctga
     3781 tttacgcatg gaaagcggga cgtaacacct ggtggtttgc agccacgtta acggtgctgg
     3841 ggctatttgt cgttttaaat atcaccctgt ttgccagcga ctattttact ggcgatggta
     3901 ttaacgacgc ggttctctat accttaacca acagcctgac cggtgctggt gtcagcaaat
     3961 acatcctgcc tggtatcggc attgtgctgg ggctgacggc ggtgttcgtt gcgctgggct
     4021 ggatcctgcg ccgtcgtcgt catcatccgc accattttgg ttacagcctg ctggcactct
     4081 tactggcgct gggttcagtg gatgccagcc cggcatttcg tcagataacg gaactggtga
     4141 aatcccagtc acgcgacggc gacccggact ttgcggctta ttataaagag ccgtcgagaa
     4201 ctatccctga cccgaaactc aacctggttt atatctacgg cgaaagtctc gagcggacct
     4261 attttgataa cgaggctttc ccggatctca cgcctgaact gggcgcgttg aaaaatgaag
     4321 gcctggattt cagccacacg cagcagttgc cgggaacgga ttacacgatt gcgggcatgg
     4381 tggcttctca gtgcggcata ccgctgtttg ccccatttga aggcaacgcc tccgcttctg
     4441 tttccagctt cttcccgcag aacatctgtc tgggcgatat tctgaaaaac tcgggttatc
     4501 agaactattt cgtgcagggc gcgaatctgc gttttgccgg taaagatgtg ttcctgaaat
     4561 cgcacggctt cgaccactta tacggctcag aagagctgaa aagcgtggtg gccgacccgc
     4621 actatcgcaa cgactgggga ttctacgacg ataccgttct cgatgaagcg tggaaaaaat
     4681 ttgaagagct ttcccgctca ggtcagcgat tctcactgtt taccctgaca gtcgataccc
     4741 atcacccgga tggttttatc tcgcgcacct gtaaccgcaa aaaatatgat tttgacggta
     4801 agccgaatca gtcattcagc gcggtaagtt gcagccagga gaatatcgcg acgtttatca
     4861 acaaaatcaa agcgtcaccg tggtttaaag ataccgtcat cgtcgtctct tctgaccatt
     4921 tagcgatgaa caatacggcg tggaaatacc ttaataagca ggatcgcaat aacctgtttt
     4981 ttgtcatccg tggcgacaag ccgcagcaag agacgctggc agtgaagcgt aatacgatgg
     5041 ataacggcgc gacggtgctg gacattctcg gtggcgataa ctatctcgga cttggccgta
     5101 gcagtttatc cgggcagtcg atgtcggaaa tcttcctcaa tatcaaagag aaaacattgg
     5161 cgtggaagcc ggatatcatc cgcctgtgga aattccctaa agagatgaaa gagttcacca
     5221 tcgaccagca gaaaaacatg attgccttct caggtagcca tttccgtttg ccgctgctgt
     5281 tgcgggtttc agacaaacgc gtggaaccgc tgccggaaag cgaatactca gcaccgctgc
     5341 gtttccagct ggcagatttc gctccacgcg acaatttcgt ctgggttgac cgttgctaca
     5401 agatggcaca actctgggct ccggaactgg cactctccac cgactggtgt gtctcgcaag
     5461 ggcagcttgg cggtcagcaa attgttcagc atgttgacaa agcaatatgg aagggcaaaa
     5521 cggcatttaa agatacggtc atcgacatgg cgcgttacaa aggcaatgtc gatacgctaa
     5581 agattgttga taacgatatt cgctacaaag ccgacagttt catctttaac gtcgctggtg
     5641 cgccagaaga ggtgaaacag tttagcggga tttctcgtcc agagtcgtgg ggccgctggt
     5701 ccaacgcaca gctgggcgct gaagtaaaaa tcgagtacaa gcatccgctg ccgaagaaat
     5761 ttgaccttgt gattaccgcc aaagcatacg gcaataacgc cagccgtcct attccggtac
     5821 gcataggcaa tgaagaacaa acccttgtgc tgggcaatga agtgaccacc accacactgc
     5881 atttcgataa cccaaccgat gccgacacac tggtaattgt gccgccggaa cctgtctcaa
     5941 ccaacgaagg gaatatcctc ggacactcac cgcgtaagct cgggatcggc atggtggaaa
     6001 ttaaagtggt agaacgtgaa gggtaattcc tgactgatgt gtaggtcgca taaggtgcga
     6061 cctacacgcc ttctccggct tacaacacat tattaagaaa caaaaaaaca gcgcccaaca
     6121 cttccctgga cgctacatgg caacttgaat aatctcgcct tatcagaaag taattacgcc
     6181 cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gtgtggcgaa
     6241 cggatagcgg tgggttaaca tcatgtcggc agtgattttc ccttccgcca tcaatagacc
     6301 gactttagcg aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt
     6361 atggaattcc ggatcggaga actgcaactc acctttaaac aggccgacaa ataccaccgt
     6421 gccaccgtga cgaatcagat ttacggtgtt attcatcgca tgttgattac ctgtcgcgtc
     6481 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgc agctgcgcgt caaaatcttc
     6541 ggctgacggg tccagaacag gtaattccag acgcgttgcc acatgttcac ggcgcgccgg
     6601 actggtatcc gccaccacca cctgcgcgcc atcggcttta gcgattgccg ccgcgcccag
     6661 accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
     6721 caccgcatga gcgctaatag cgaaaggttc aatcaatgct gccgcctgcg ggtcaatacc
     6781 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
     6841 atgcacgcca atgactgaaa ttttttcaca gcaattggta cgaccgcttt tacacgccgg
     6901 gcattgctga caagaaatat acggaattac cgcaacttgc tgtccgatat taaaattgtg
     6961 aatgtttttt cctgattcaa taatctcccc acatatttca tggcctaaaa cgcgtggata
     7021 actaaaaaat ggctgattac ccgcccaggc atgaatatct gtaccgcaaa tcccgacagt
     7081 tttaatttta attaatactt cattatcgcc tggcaccggt ctttcccgga cttcccatac
     7141 taaattcttt atttcctgac aaattaatac tttcattgtg gacatgattc accgctcctt
     7201 ttattttatg actttcagat atcgtcgaat ttataagagc gcctgaattt acccataaaa
     7261 tatgcggatg atcgcgcata aaaatgtttt aaatcggctt ttaatgccgg actggaggga
     7321 agaatgagtc gttcgcagaa tttacgtcat agcgtcataa atcaggtgat tgaagatatg
     7381 gcgaggggca atattccttc tccgctgcct tcacaaagcg gactggcaga aatgtataac
     7441 atcagtcgca ctacggtacg ccacatactc cagcatttaa gcgcatgtgg cgtgctcacc
     7501 ctggtgggga aaaattatgt tatcgcccgc aaacccgaac agaacgatgg atttgattgc
     7561 atcacggcct cgctgaaaga gcaaaaccgt cttttcgagc aggcattttt caccatgatt
     7621 aacctgcgcc agttacgcgc cggagaaagc ttttccgaac ttcagctggc gcgagcggca
     7681 agcgtcagtc cggtagtggt acgagaatat cttttgaaat tcggtcgtta taatcttatt
     7741 caaagcgaaa agcgcggcca atggagcatg aagcagttcg atcaatccta cgccgagcag
     7801 ctgtttgagc tacgcgaaat gctcgaaaca cattccctgc aacactttct caaccttccc
     7861 gatgatgacc ctcgctggct gcaagcaaaa accatgctcg aacgccaccg gacgctgcgc
     7921 gacagcattg gcagcaactt tcgaatgttt tctcaattag accgtgattt tcatgccatg
     7981 ctgttatccg ctgctgacaa catctttttt aatcaatcac ttgaaattat atcggtgatc
     8041 tttcattttc actatcaatg ggatgaaagc gatctcaaac agcgcaatat cattgctgtt
     8101 gatgaacata tgaccattct cagcgccctg atttgccgta gcgatttaga tgcaataacg
     8161 gcgttgcgaa accatctgga taccgccaaa caatcaatga ttcgttccat caggcaacat
     8221 caccgataaa ttaagtacag attaaaaaca ttgattcttg gtttatatca ctatgttgta
     8281 ttaatgcagt attaatccag gcgattgttc tgcccataag gaaatacata gggaataaac
     8341 gatgctgaaa gtattactct cagccatcat ttaattccct attactggca gcgataaaat
     8401 tatcggctat ccatgattaa cgtttccagc acatctaatt cactgactat ttttgccagt
     8461 gtttcatcac tgacttcttg ccttgcgcgc atctggtaaa tagccgcgcg tttggcattt
     8521 aacgccgcca gattaaatgt tctttccagc ttcgcacgct gcatatgcag ggaattatca
     8581 ttaggcgaca tatcctcttc actatgtcgc atataaccga taactctggc ccccgtttcc
     8641 ataatcaact cgttatcgag gttttctgtt ctggcgcgca gcaaacgttg ctcggtcttt
     8701 tgcacactca gtatggccat tttagccata aacagatcgg cagcttcctc ttctttttgt
     8761 cggttatcgc tgaaaatccc cacatttttc cctagtaata acggcagaat aataatgccg
     8821 caaaccagcg aaaagaggat cacacctgta gcgagaaata ccagcaagta acgcccggga
     8881 aaaggtgtgc catccggcag caataacggg atagaaagca cccccgcaag cgtgatagct
     8941 ccgcgcaccc ccgcaaaggt cgccagcagc atatgacgcg ttttatatcc agcaaacagc
     9001 atcggatgtt tgccgggtcg tttacggcta tacagacgca tcagccaaag ccagcccagg
     9061 cgcacaacca ttaacgccag gtagatccaa atgacatcaa ggagcagcga tccggtgtcc
     9121 accagtggat cgttttctgc acggatcagt gattccgaaa taatgcccgg cagttctaag
     9181 cctaaaatca gaaacaccat gccgttaaag acatattcaa gcatcgacca gctactttta
     9241 gctttaattc tcaggcgaat aggcgcgtgg tggactacgc caatgcgact ggttgttaac
     9301 cccgcggcga cagcggcaag aatgcccgat aagccaaaat gttccgcaac cagataaaca
     9361 ataaacggca acagaagcat caatacaatc tgaatggaag gttcatcgtg agcatatcgg
     9421 ctgatcaaca gcagcccttt accatataac caggtaacag cgatccccac ggcaataccg
     9481 cccaacgcaa cgacaaaaaa cgcaatggtt gcgccatgaa cgctgaattc catcgttccc
     9541 atcgtcactg caacagcaaa tttcaacgcc accaggccgg aagcatcatt catcaacgct
     9601 tcgccttcga gcacttcctg gatgtttttg ctgatacgcc ctttcccgac aatccccagc
     9661 aacgccacgg catcggtagg cgacaacacg gcggctaatg cgaaagcagc gatgagcggc
     9721 acattcggta agagtaaatg aatcacatag ccgacaccaa aaatagtgat tagtaccaac
     9781 acaagtgcca ggctgataat ttcccggctg tgcttgatca attctttagg ttgtattttt
     9841 gaactttcag caaacaacag gggtggtata aacaataaga gaaaaagttc aggattgaat
     9901 tccacatgca gaccaaatat aggctgggcc agtactgcgc cagcggcaat ctggattaaa
     9961 ggaagtggta tctggacagg agacagacgt gtaagcacgc tggttaatga tactaccaac
    10021 gtaagaatta aaattgttaa aagtatttcc atataataaa caggcttcct cattcataaa
    10081 taatggatat tattaattta atttcataaa aagcgctctg gttagcactt aacgttaatc
    10141 attaatcact gcgttcactc acatgatcag aaggaaccca gtaaaatgag ttttcaagtt
    10201 gataacaaat tcaccatgtg aatatttact acctttcccc gttgtaacga atcatccgtt
    10261 actctctgat ttccttgccg aacaaattat ttagtgaatg acggctttct tttaatatca
    10321 gcatgaatca tacgttcagc aaatgtatta aatcagcact aaataaattt aatggatacc
    10381 accattctgc gtcaacactt agtttttatc tg
//
LOCUS       DAJOTB010000156        10376 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJOTB010000156 DAJOTB010000000
VERSION     DAJOTB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.156"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..263)
                     /locus_tag="OHN28_004022"
     rRNA            complement(<1..263)
                     /locus_tag="OHN28_004022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(707..3280)
                     /gene="clpB"
                     /locus_tag="OHN28_004023"
     CDS             complement(707..3280)
                     /gene="clpB"
                     /locus_tag="OHN28_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCQ2299321.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3410..4141)
                     /gene="pgeF"
                     /locus_tag="OHN28_004024"
     CDS             complement(3410..4141)
                     /gene="pgeF"
                     /locus_tag="OHN28_004024"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCQ2299322.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(4138..5118)
                     /gene="rluD"
                     /locus_tag="OHN28_004025"
     CDS             complement(4138..5118)
                     /gene="rluD"
                     /locus_tag="OHN28_004025"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCQ2299323.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            5253..5990
                     /gene="bamD"
                     /locus_tag="OHN28_004026"
     CDS             5253..5990
                     /gene="bamD"
                     /locus_tag="OHN28_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCQ2299324.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            6260..6601
                     /gene="raiA"
                     /locus_tag="OHN28_004027"
     CDS             6260..6601
                     /gene="raiA"
                     /locus_tag="OHN28_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCQ2299325.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6704..6829
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6705..6752
                     /gene="pheL"
                     /locus_tag="OHN28_004028"
     CDS             6705..6752
                     /gene="pheL"
                     /locus_tag="OHN28_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCQ2299326.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6851..8011
                     /gene="pheA"
                     /locus_tag="OHN28_004029"
     CDS             6851..8011
                     /gene="pheA"
                     /locus_tag="OHN28_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCQ2299327.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELVVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(8054..9175)
                     /gene="tyrA"
                     /locus_tag="OHN28_004030"
     CDS             complement(8054..9175)
                     /gene="tyrA"
                     /locus_tag="OHN28_004030"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCQ2299328.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLILSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(9186..10256)
                     /gene="aroF"
                     /locus_tag="OHN28_004031"
     CDS             complement(9186..10256)
                     /gene="aroF"
                     /locus_tag="OHN28_004031"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCQ2299329.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2628 a         2769 c         2406 g         2573 t
ORIGIN      
        1 ttaccccacc tactagctaa tcccatctgg gcacatccga tggcaagagg ccctaaggtc
       61 cccctctttg gtcttgcgac gttatgcggt attagctacc gtttccagta gttatccccc
      121 tccatcaggc agtttcccag acattactca cccgtccgcc actcgtcagc gaaacagcaa
      181 gctgtttcct gttaccgttc gacttgcatg tgttaggcct gccgccagcg ttcaatctga
      241 gccatgatca aactcttcaa tttaaaagtt tgatgctcaa agaattaaac tttgtaatga
      301 attacgtgtt cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg
      361 tatcttcgag tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct
      421 ttttctcagc ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat
      481 ttttgctttc tctgccggaa ttctcaggag aaccccgctc acccggcggc gtgtttgccg
      541 ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca agcgaaaaat
      601 ataaaaactt tatcgttcgc tcatttttca ggcaaaacat cttaaatata gtcttttccg
      661 tctaacttat agacaaaaac gagccccgaa gggctcgttt tatcatttac tggacggcga
      721 caatccggtc ttcattaact tccaggcgaa tcactttacc cggaaccaat tcaccagaca
      781 gtatttgctg tgccagcggg ttttcaatct gctgctgaat tgcacgtttc agaggacgtg
      841 cgccatagac cggatcgtaa ccgttctcgc tcagcagttt cagcgcctcg tcagaaatgt
      901 ggatttcata accacgttct tccagacgtt tatacagacg tttcaactga atctgcgcaa
      961 tcgacgcaat gtgctgttca cccagcggat ggaagaccac cacttcatcg atacggttaa
     1021 tgaattccgg acggaagtta tggctaacca caccgagcac cagctctttc atgtgcgcat
     1081 aatccagttc accgaagcgt tcctgaatca aatcggaacc gaggttagag gtcataatga
     1141 caaccgtatt acggaagtcg accgttctcc cctgcccgtc agtcagacgc ccatcatcca
     1201 gtacctgcaa cagaatgttg aagacatccg gatgcgcttt ttccacttca tccagcagaa
     1261 tgacggaata cggacgacga cgcaccgctt cggtcaggta gccaccttct tcataaccga
     1321 catatcccgg aggcgcacca accagacgag acaccgagtg tttctccata aactcggaca
     1381 tatcgatacg gaccatcgcc tcgtcgctat caaacataaa gttcgctagc gccttacaaa
     1441 gctcagtttt ccccacaccg gttgggccga ggaacaggaa tgaaccaatc gggcgatttg
     1501 gatccgccag ccccgcacgg ctacgacgaa tagcgttaga taccgcatca accgcttcgt
     1561 tctgaccaat aacgcgatgg tgcagttctt gctccatacg cagcagtttt tcgcgctcgc
     1621 tttccatcat gcgagaaacc ggaatccccg tccaacgcgc cagcacttca gcaatttcgg
     1681 catcggtcac tttattacgc aacagacgca tagttttgcc ttcgagctgc gttgcggctt
     1741 ccagttgctt ttccagttcc gggattttgc cgtattgcag ttcagacatc cgcgccaggt
     1801 cacccacacg gcgagcctgt tcaatagcaa ttttcgcctg ttccagttcc gctttaatgg
     1861 tctgcgtacc agaaagcgag gctttctctg ctttccactc ttcttctaac tcggagtact
     1921 gacgttcttt gtcgctcagt tcttcgttga gcatatccag gcgtttttta ctggcttcat
     1981 cagactcttt cattaacgcc tgttgttcca gtttgagctg gatgatacgg cgatcgagtc
     2041 ggtcgagttc ttctggtttt gagtcaatct gcatacgaat gctggatgct gcttcatcga
     2101 tcaggtcgat ggctttatcc ggcagctgac ggtcagcaat gtagcgatga gacaacgtcg
     2161 ctgctgcaac aattgccggg tcagtaattt gcacatggtg gtgcagctcg taacgttctt
     2221 tcaggcctcg cagaatagca atggtatctt caacagaagg ctcggcaaca aacactttct
     2281 ggaaacgacg ttccagcgca gcatcttttt cgatgtactg gcgatattcg tcaagcgtcg
     2341 tggcacctac gcagtgcaat tcaccacgcg ccaacgctgg tttcagcatg tttccggcgt
     2401 ccattgcgcc atcggcttta cccgcgccga ccatggtatg taattcgtcg ataaacagga
     2461 tgacgttgcc ttcctgttta gcaagatcgt taagtacgcc ttttaaacgt tcttcaaact
     2521 caccgcgata tttcgcccca gccaccagcg cgcccatatc cagcgccagt acgcggcggc
     2581 ctttcaaccc ttcgggcact tcgccgttga taatacgctg cgccagacct tcaacgatgg
     2641 cagttttacc gacgccgggt tcaccaatca ataccgggtt atttttagta cgacgttgca
     2701 gcacctgaat ggtacggcga atttcttcat cacgaccaat caccggatcg agtttgcctt
     2761 gttcggctcg ttcggtaagg tcgatggtat attttttcaa agcctgacgt tggtcttcag
     2821 caccttgatc gttcacgctt tcacctccac gcatttgttc aatcgcttga gtaatgttgg
     2881 cggtggtcgc ccctgctgct ttcaggatgt cggccagcgt gccgcgagac tcaagtgccg
     2941 ccagaacgaa cagttctgac gagataaagt tatcaccacg tttttgcgcc agcttgtcgc
     3001 aaagattaag aacgcgcacc agatcctgtg atggctggac atcaccacca gtaccttcaa
     3061 cctgtggtaa acgatttaat gcctgattga tatctgtgcg caactggcca gcattaatgc
     3121 cagcggatgt taataaagga ctaaccgaac ccccttcctg attcagcagg gcgctcatta
     3181 aatgaagtgg ttcgatgaat tggttgtcgt gcccgagtgc aagtgattgg gcatcggcaa
     3241 gagcaagctg gaatttatta gtaagacgat ccagacgcat aactcctccc ataacggatc
     3301 aaagttgcta ctggagatta aatgaggtca tccctcaatt attcaaggtt attgaccaga
     3361 ttaatgtgaa aagaaaatca cgcgtaccgg atcgtcttga ttctttaggt tatatcagcc
     3421 aaatgaaact tgccatacga ccggtggtct tgtcgcgacg ataagagaag aaagtctcat
     3481 tttccgtata tgtacaacgg tcgccgccga aaatttgttc aacaccaacg ctcgccagac
     3541 gctgccgggc aagctgataa atatccgcca gatacttatc accatgctga atgaaagctg
     3601 tactcgcttt ggcgtctact gccataaacg cctcgcgaac ctccgccccc acttcgaacg
     3661 cacgtggccc aattgccggc cctaaccagg cgagaatatt ttccggatta tcagcaaaac
     3721 aggaaaccgt ctcttccaac acgcctgcgc acagtccacg ccagccagca tgagcggcgg
     3781 cgacctccgt tcccgcacga ttgcaaaaca gcacgggcag gcagtcggca gtcatcactg
     3841 cgcaaaccgt gccaggcgta ttgctataag aggcatctgc tcgttttgag gcataaggtt
     3901 cgccaataag cttaagcacg tctttgccgt gtacctgctc aagccagacc ggtttagaag
     3961 gcaaattgcc cgcagcaaaa agtcgcttgc gattctcccc aacgtgatcc gggttatcgc
     4021 cacaatgggc accgaggttg agcgagtcat acgggggtaa gctcacaccg ccgatacggg
     4081 tagagctaca agccgcaaca ccttttggca gcggccactg cgggacaatc agcttactca
     4141 taaccagtcc acttcatcct tatgctcttc gaaatcggcg cgcatctcct caatcagctc
     4201 caccatatct tgtggaatag gcgcatgcca ttccatttcg atgccggaga tcgggtgata
     4261 aagacgcagc atggttgcat gaagcgcctg acggtcaaac ttacgcagcg tggagataaa
     4321 cgcttccgaa gcaccttttg gcggacgcgg acgtccacca taaaccggat cgcccaccag
     4381 cggatgagtg atatgggcca tatgcacgcg gatctggtgc gtacgtccgg tttccagacg
     4441 caaccgcaga cgcgtgtgca cacggaagtg ttccatgatg cgatagtgag tgaccgccgg
     4501 tttacccatg ggatgtaccg ccatgtgagt acgtttggtc gggtggcggc tgattggttc
     4561 atctactgta ccgccagcgg tcatatgacc aattgccacc gcttcatact cacgtgtaat
     4621 ttcacgccgt tgcaaagatt cgactaaacg cgtctgagcc ggaacggttt ttgccacaac
     4681 catcaggcca gtggtgtctt tatccagacg atggacaatg cccgcacgcg gtacatcggc
     4741 aatgggcgga taataatgaa gcaatgcatt cagtaccgtg ccatccgggt tacccgcgcc
     4801 aggatgtacc accaggtcgc gcggtttatt aataacgatg atatcttcat cttcatagac
     4861 gatatccagc gggatatcct gcggttcaaa acgcgcttct tcttcaatct cagcgttgat
     4921 ggcaacctgc tcgccaccca atactttttc tttcggctta tcacacactt tgccgttaac
     4981 cagcacacgc tggtcgagga tccattcttt tatccgcgaa cgtgaataat ccgggaacat
     5041 ttcggccaaa gcctgatcta agcgttgacc gagttggttt tcggacaccg ttgcagtgag
     5101 ctgtactcgt tgtgccatat atactgcttc ttcgtttatc gttggggttt tacggctttg
     5161 ccgtttaata tagtgtgcta ttgtagctgg tcttaaccgg gagcaggaac agagaatctc
     5221 ccgtattaca ttttgaggaa agtcaaaacg tcatgacgcg catgaaatat ctggtggcag
     5281 ccgccacact aagcctgttt ttggcgggtt gctcggggtc aaaggaagaa gtacctgata
     5341 atccgccaaa tgaaatttac gcgactgcac aacaaaagct gcaggacggt aactggagac
     5401 aggcaataac gcaactggaa gcgttagata atcgctatcc gtttggtccg tattcgcagc
     5461 aggtgcagct ggatctcatc tacgcctact ataaaaacgc cgatttgccg ttagcacagg
     5521 ctgccatcga tcgttttatt cgccttaacc cgacccatcc gaatatcgat tatgtcatgt
     5581 acatgcgtgg cctgaccaat atggcgctcg atgacagtgc actgcaaggg ttctttggcg
     5641 tcgaccgtag cgatcgcgat cctcaacatg cacgagctgc gtttagtgac ttttccaaac
     5701 tggtgcgcgg ctatccgaac agtcagtaca ccaccgatgc caccaaacgt ctggtattcc
     5761 tgaaagatcg tctggcgaaa tatgaatact ccgttgccga gtattataca gaacgtggtg
     5821 catgggttgc tgtcgttaac cgcgtagaag gcatgttgcg cgactatccg gatacccagg
     5881 ctacgcgtga tgcgctgccg ctgatggaaa atgcataccg tcagatgcag atgaatgcgc
     5941 aagctgaaaa agtagcgaaa atcatcgccg caaacagcag caatacataa cagaaacctg
     6001 aaacacaaaa cggcagcctt gagctgccgt ttttttattc tgttagttgt gaaactgaag
     6061 cgatttagtc actatcgatc tcatcaaata tggcccgctt tgagatattc ctcaagtaaa
     6121 aaaacacctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag tgacaaaatt
     6181 atgagatttc catcacacat tttgacatca ggaacgttat gctgaattca ccaagacggg
     6241 aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg aaattactcc
     6301 ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc aaacacatct
     6361 gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg acgccacaat
     6421 caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt acaccgcaat
     6481 taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca aaggcgaagc
     6541 acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg aagaagagta
     6601 gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt tgacagcgtg
     6661 aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa cacacaccgt
     6721 ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg tgtgaaacag
     6781 aatgcgaaga cgaacaataa ggcctcccaa atcggggggc cttttttatt gataacaaaa
     6841 aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagaaaaaa tcagcgcgct
     6901 ggatgaaaaa ttattagcat tactggcaga gcgacgcgaa ctggtcgtcg aggtgggaaa
     6961 agccaaactg ctttcgcatc gcccggtacg tgatattgat cgtgaacgcg atttgctgga
     7021 aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta ctcgcctgtt
     7081 ccagctcatc attgaagatt ccgtattaac tcagcaggct ttactccagc aacatctcaa
     7141 taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt cttattccca
     7201 tcttgcggcg cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa gtggctgcgc
     7261 caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg ccgtcgtacc
     7321 gattgaaaat accagctccg gtgccataaa cgacgtttac gatctgctgc aacataccag
     7381 tttgtcgatt gttggcgaga tgacgttaac tatcgatcat tgtttgttgg tctccggcac
     7441 tactgattta tccaccatca atacggtcta cagccatccg cagccgttcc agcaatgcag
     7501 caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta cgtctgcggc
     7561 aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttgggaa gcgaagctgg
     7621 cggcactttg tacggtttgc aggtactgga gcgtattgaa gcgaatcagc gacaaaactt
     7681 cacccgattt gtggtgttgg cacgtaaagc cattaacgtt tctgaccagg ttccggcgaa
     7741 aacgacgttg ttaatggcga ccggacaaca agctggtgca ctggttgaag cgttgctggt
     7801 actgcgcaac cacagtctaa ttatgacccg tctggaatca cgtccgattc acggtaatcc
     7861 gtgggaagag atgttttatc tggatattca ggccaatctt gaatcagcgg aaatgcaaaa
     7921 agcattgaaa gagttagggg aaatcacccg ttcgatgaag gtattgggct gttacccaag
     7981 tgagaacgta gtgcctgttg atccaacctg atgaaaaagt gccggatgat gtgaatcatc
     8041 cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg cggctttcac
     8101 tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa ctgtcaataa
     8161 acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc ttatagtaac
     8221 gtttgattaa cgccagatta cgctctgacg acataataat gtcggcataa agctgcggat
     8281 cctgagcaaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc gaagagagcg
     8341 ccagtagttg ctcaagctga acattttctt ctgccaggtg cagcccataa gcaaaagtag
     8401 caaagtggcg cagagcctga ataaacgcca tattctgatc gtgctcgaca gcgctaatac
     8461 gatgcaaccg agcgccccag acctgaattt gctccagaaa ccattggtat gcttccggct
     8521 tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc gggccgaaca
     8581 tcgggtgtaa ccccagcacc gggccatcgt gcgccgccag catggcctgt aatggtccat
     8641 ttttcactga tgccagatca accagaatac aatctttcgg taaaggcggt aatttgccga
     8701 taacttgctc agtaacgtgg atgggcacac taacaatcac cattccggca tcggcaacaa
     8761 tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa cccgagagta
     8821 tcagcatctt ctcgaacaga cgtcccatct gaccgccacc accgacgata accaccgggc
     8881 gcagtgacgg acaaagcgtt ttaaatcctt tgtcgttttc actggagtaa gattcacgca
     8941 tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct tccgcctccg
     9001 cacgacgcga ggccaacata gatgcctcgc gctccggaac ataaataggc agtccaaatc
     9061 ggcttttcac ctcgcccact tcagcaacca attccagacg cttcgctaat aaattcagca
     9121 gcgctttatc gacttcatca atttgatcgc gtaatgcggt caactcagca accataataa
     9181 acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat ttcacgcagc
     9241 aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc gtatttcatt
     9301 tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc gatcatcaga
     9361 ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc caccgcaggc
     9421 tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag agacgggcgc
     9481 agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct atagtttggc
     9541 gctttaccac cgcgcaggat cacatgcccg tccggattcc cctgagtttg tagcaacgca
     9601 acctgccctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc gcgcatagcg
     9661 ttaattgccg ttgccagact gccatcggtg ccgtttttaa aaccaaccgg catggaaagc
     9721 ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat tgctgaccag
     9781 ctaaacagat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt cgccagtggc
     9841 agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc ggcttctaca
     9901 tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac agtggtacgg
     9961 ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc tgcggcaagg
    10021 gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga acaaggacca
    10081 catactacca gaagacgagg atcgcgcccg gcgataatat ctgaaatggt tttacgcgag
    10141 tcagcaatct gggcttcttg ttgcaggctt aatggaaaag cggccttcag ttgttccgga
    10201 gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt ttgcatgatg
    10261 gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac gtaacgataa
    10321 cacataaagt aaagttttca atccatattt cgtacacatt tctttacgta agcaat
//
LOCUS       DAJOTB010000157        10278 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJOTB010000157 DAJOTB010000000
VERSION     DAJOTB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.157"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            23..388
                     /locus_tag="OHN28_004032"
     CDS             23..388
                     /locus_tag="OHN28_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCQ2299330.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            535..1053
                     /locus_tag="OHN28_004033"
     CDS             535..1053
                     /locus_tag="OHN28_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCQ2299331.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1043..2269
                     /gene="dgcN"
                     /locus_tag="OHN28_004034"
     CDS             1043..2269
                     /gene="dgcN"
                     /locus_tag="OHN28_004034"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCQ2299332.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2285..2767
                     /locus_tag="OHN28_004035"
     CDS             2285..2767
                     /locus_tag="OHN28_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCQ2299333.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLITQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2844..3191)
                     /gene="rplS"
                     /locus_tag="OHN28_004036"
     CDS             complement(2844..3191)
                     /gene="rplS"
                     /locus_tag="OHN28_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCQ2299334.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3233..4000)
                     /gene="trmD"
                     /locus_tag="OHN28_004037"
     CDS             complement(3233..4000)
                     /gene="trmD"
                     /locus_tag="OHN28_004037"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCQ2299335.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4031..4579)
                     /gene="rimM"
                     /locus_tag="OHN28_004038"
     CDS             complement(4031..4579)
                     /gene="rimM"
                     /locus_tag="OHN28_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCQ2299336.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4598..4846)
                     /gene="rpsP"
                     /locus_tag="OHN28_004039"
     CDS             complement(4598..4846)
                     /gene="rpsP"
                     /locus_tag="OHN28_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCQ2299337.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4983..6344)
                     /gene="ffh"
                     /locus_tag="OHN28_004040"
     CDS             complement(4983..6344)
                     /gene="ffh"
                     /locus_tag="OHN28_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCQ2299338.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6511..7302
                     /locus_tag="OHN28_004041"
     CDS             6511..7302
                     /locus_tag="OHN28_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCQ2299339.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            7323..8609
                     /locus_tag="OHN28_004042"
     CDS             7323..8609
                     /locus_tag="OHN28_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2299340.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8663..9256)
                     /gene="grpE"
                     /locus_tag="OHN28_004043"
     CDS             complement(8663..9256)
                     /gene="grpE"
                     /locus_tag="OHN28_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCQ2299341.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9379..10257
                     /gene="nadK"
                     /locus_tag="OHN28_004044"
     CDS             9379..10257
                     /gene="nadK"
                     /locus_tag="OHN28_004044"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCQ2299342.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
BASE COUNT         2525 a         2712 c         2404 g         2637 t
ORIGIN      
        1 tgctttgaaa aaggagaaag aaatgatgaa aaaatttatc gcccccttgt tggctttact
       61 ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca gcaccgactg
      121 gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc attaaagatg acgatgcatt
      181 tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag ggcaaaagaa
      241 aacttgccag accgatttta cttatgcccg ggggctttct ggtaaaagct ttcctgccag
      301 ctgcaataat gttgaaaatg ccagccaact gcatgaagta tggcgaaaag gggctgatga
      361 aaatgccagc acaatacgat taaattaatt acccccaaaa taaattaagc atgagatttt
      421 actcattatg ttttatttct cacattgatg accaccgcat gaaaggatga tattttaatg
      481 agcattctga aaaaatatcg atgcatttcg agcgaagatg gtgaggatcc ctgaatgcgt
      541 ttttctcacc gactgttctt actgctgatt ctcctgctga cgggagcacc aatactcgct
      601 caggaacctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac
      661 acccgctggc ctgggctatc agggccacca aagttatgta tcttttcatc ttcacgtttt
      721 agtaccgcct tacaagaaaa tgctgctaca tctttacctt atctgcctgt catcattcat
      781 actcaacaag aggcgataat ttcaggctgc aatggttttt attttggcaa cgagtcaccg
      841 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa
      901 caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa taatgacgtc
      961 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
     1021 ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttcata agcgccccac
     1081 gtttaaaaga gcattacgca acatcagcat gaccagcata tttatcacta tgacgctgat
     1141 ctggctgctg ctttccgtga cgtcagtgct aaccctgaaa caatacgcgc aaaaaaacct
     1201 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
     1261 ccctgcagca actgaaacac tggcagcgct gggccagcaa ggccaatttt caactgcaga
     1321 agtgcgtgat aagcagcaaa atattctggc atcctggcat tacacccata aggaaccagg
     1381 cgatactttc agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat
     1441 tcgtcacaat agtgaaacca ttggcgaagt acgcttaact gctcgcgaca gttcaatcag
     1501 ccattttatc tggttttcgc tcgctgtcct gaccggttgt attctgctgg catcaggcat
     1561 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcgctga aaaatatcac
     1621 cgatgtcgta catgatgtgc gttctaaccg caatttttcc cgacgagttt cggaagaacg
     1681 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
     1741 gtggcagctt cgtttacagg ccaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc
     1801 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa
     1861 ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat
     1921 caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatag caaaacggtt
     1981 agctgaattt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat
     2041 ggtgctctat gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca
     2101 aatttttaat ctcccgtttg atcttcataa tggccatcaa actaccatga cattaagcat
     2161 tggttacgcg atgaccattg agcacgcctc tgcggaaaat ttacaagagc ttgccgatca
     2221 caatatgtat caggccaaac accagcgtgc cgaaaaactg gtgagataac aaggatatat
     2281 cgatatgata aagcacctgc tagcccccct gattttcacc tcgctaatac ttactggctg
     2341 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
     2401 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
     2461 tgactacaaa ttgctcccgg aaagccagca acaaatccaa accatggcag ccaaattggc
     2521 ctcgacaggg ctaacacatg cccgtatgga tggacacacc gacaactatg gtgaagacag
     2581 ttacaacgaa gtcttatcat taaaacgggc gaatgtcgtg gccgatgcat gggctatggg
     2641 tggacaaatt ccgcgcagca atctcatcac acagggttta ggaaaaaaat atcccatagc
     2701 cagtaacaag accgcccagg gccgcgccga aaaccgccgc gtcgcagtgg tgattacaac
     2761 cccttaaaac acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa
     2821 caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca
     2881 gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa
     2941 atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa
     3001 attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaattgcgat aaccacgccc
     3061 tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta
     3121 tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata
     3181 atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg gcttacgcca
     3241 tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg
     3301 cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc
     3361 ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg
     3421 gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc
     3481 cttcagcaaa ggagtcttcc gttgccgagg cctcatgtcc cagtactccc ggaataaacc
     3541 gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc
     3601 cgattgacca ttcttcatca atttcggttt ggatcacgcg ctcatctata ccttcgtagc
     3661 gaccgcacac cagaatcaat ttttgattcg ttgccagctc gctgacgccc gcttgatcaa
     3721 gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg
     3781 ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt
     3841 aaggacgatc gtccacggta cggtgccgat catacgtgaa gtcgcgagga ctccagctct
     3901 ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc
     3961 ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt
     4021 atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga
     4081 ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat
     4141 ctttcaggtt tgccttaatg acgagaacgt cattagatcc ggtttccatc atatctacga
     4201 ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt
     4261 agtagtcacc ctcttcaagc tgaggcaact gcgatgaatc cacgacaatt tcacaattcg
     4321 tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat
     4381 tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc
     4441 agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc
     4501 cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg
     4561 cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt
     4621 tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc
     4681 gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa
     4741 cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga
     4801 acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg
     4861 tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa
     4921 aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc
     4981 atttagcgac caggaaatcc cggcggcatc atccctttca tgcttctcat catcttcgcc
     5041 atcccgccct tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg tttcagaaga
     5101 cggttaacgt cctgcacctg cataccgcaa ccggcagcga tacggcgctt acgcgaacct
     5161 ttgatgattt ctggcttagc gcgctctttc atcgtcatcg agttgatgat ggcttccata
     5221 cgcaccagca ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc
     5281 ggcagcttgc ccatcaggct agccatgccg cccatatttt tcatctggcg cagctgctca
     5341 agaaagtcgt tgagatcgaa gccgtcgcct tttttcagct tgctggctaa tttctcagct
     5401 tgcgcgcggt caactttgct ttcgatatct tcgatcagtg acagtacgtc gcccatgccg
     5461 agaatacggg aggcaatacg gtccggatgg aatggctcca gcgcctcggt tttctcgcca
     5521 acaccgagga acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg
     5581 cgggcatcgc cgtccacttt ggtcaacact acaccggtaa gcggtaacgc ttcattgaat
     5641 gcttttgccg tattggccgc atcctgaccg gtcatggcat cgaccacaaa tagggtttca
     5701 accggattaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
     5761 agacgaccgg cagtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
     5821 gccgcgttaa cgatatctac cggcttctga ccaacatcag aagggaagaa atcaacgccc
     5881 acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcggcagaa
     5941 accaccagta ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg
     6001 gttgttttac cggcaccttg caggcccgcc atcagcacga ccgcaggcgg ttgcgcagcc
     6061 aggttcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacaattttg
     6121 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
     6181 acgcgattga taaactcacg cactaccggc agagcgacgt cagcctccag cagcgccatg
     6241 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
     6301 ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg
     6361 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
     6421 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
     6481 tttcttattg ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc
     6541 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
     6601 cggcgcatgg ctattatttc tgcggtcatc gcgctggtct gccacgcaat cgctctggaa
     6661 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
     6721 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
     6781 ttgttgctac ccattgtcta tgcctttgcg cttatcaacc tagcgctggc gacctttatg
     6841 cccaatgaat acatcaccca tctggaagca acgccgggga tgctggtgca cattggctta
     6901 tcgctttttt cctatgccac gctaattatc gctgccctgt acgcgctgca actggcgtgg
     6961 attgattacc aactgaagaa caagaagctg gcgtttaacc aggaaatgcc gccattgatg
     7021 agtatcgagc gtaaaatgtt ccatatcacg cagattggcg tggtgctgct aacgctcact
     7081 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggcc
     7141 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa
     7201 ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg gtgcagtcat tctgacactg
     7261 gcctacttcg gcagccgaat ttttcagcag ttaatcagct aaacccagaa aaggagtttc
     7321 ccctggaaca catttctact actacgttga tcattattct gatcatcatg gtggtcattt
     7381 cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtctgcggc
     7441 atatggcgaa acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag
     7501 accgcctgat aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc
     7561 tcggcactat tgttggaatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg
     7621 tgctgacttt tgtcgtgctg gtttttgctg aggtattgcc aaaaaccatt gccgcgctgt
     7681 acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga
     7741 tgccgctggt ctggttgctg aatgctatta cccgcatgtt aatgcgcatg atgggcatca
     7801 aaaccgatat cgtggttagt ggctctttaa gcaaagaaga attacgcacc attgtgcacg
     7861 aatcgcggtc acaaatttcc cgtcgcaatc aggatatgct gctgtcggta ctcgatctgg
     7921 aaaagatgac cgtcgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca
     7981 acgatgactg gaaatcgatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc
     8041 tctaccgtga ttcgctggac gacgccatca gtatgctgcg tgtgcgtgaa gcctggcgac
     8101 tcatgtcgga gaaaaaagag ttcaccaaag aaaccatgct acgcgccgcg gacgagatct
     8161 attttgtccc ggagggtacg ccgctcagca cacagttggt gaagtttcag cgcaacaaaa
     8221 agaaagtcgg cctggtcgtc aacgagtatg gagacattca ggggctggtg acggtcgaag
     8281 atattctcga agagattgtc ggcgatttca ccacgtcgat gtcgccaaca cttgccgaag
     8341 aggtcacgcc gcaaaacgac gggtcggtga ttatcgatgg caccgccaac gtgcgagaaa
     8401 ttaacaaagc ctttaactgg catctaccgg aagatgatgc tcgtactgtt aacggcgtca
     8461 ttctggaagc gctggaggag attccggtag caggcacccg cgtgcgtata ggcgagtacg
     8521 atatcgatat tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtaa
     8581 aaccgctgcg cgagagtgtg gcggagtaaa aaaacggccc ggcattcgca tgctggaccg
     8641 tgatttatta cgaaaacaga aattaagctt ttgccttcgc tacagtcacc atcgccgcac
     8701 gaattgtacg accgttcagc gtataaccct tctgcataat gcccagtacg ttacctggcg
     8761 caacgtcatc agattccacc attgcgatgg cctgatgcac attcgggtcc agtggtacgt
     8821 tagtttcggc gatcacttca acgccaaact tacgcacaac atccagcatc gacttcagcg
     8881 tcagctcaat accttcaacc atcgcggaca tatccgggtt agctttatca gccacttcca
     8941 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
     9001 atttgtgagc tttttcaata tccagttcag tacgacgacg caggttttcc atttcggctt
     9061 ttacgcgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
     9121 tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct
     9181 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
     9241 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
     9301 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
     9361 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
     9421 cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
     9481 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
     9541 ggcacgctcg cggagattgg gcaacaggct gatctcgcgg ttgtcgttgg cggcgacggt
     9601 aatatgctgg gcgcggcgcg cacgctcgcc cgctacgata ttaaagttat tggaatcaac
     9661 cgtggcaacc tgggtttcct gactgacctt gatcccgata acgcccagca acaattagcc
     9721 gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
     9781 cagcaagatt gccagaaacg catcagcacg gcgattaacg aagtggtact tcatcctggc
     9841 aaagtagcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttcgcag
     9901 cgttccgatg gactgattat ttcgacgcca acaggctcca ccgcatattc cctctctgcg
     9961 ggcggtccta ttctgacacc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    10021 acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg
    10081 catcgccgta acgacctgga aatcagttgc gacagccaga tagcacttcc gattcaggaa
    10141 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    10201 tacagttatt tcaatacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    10261 tacgccagcc tctttact
//
LOCUS       DAJOTB010000158        10182 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJOTB010000158 DAJOTB010000000
VERSION     DAJOTB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.158"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(122..976)
                     /gene="rclR"
                     /locus_tag="OHN28_004045"
     CDS             complement(122..976)
                     /gene="rclR"
                     /locus_tag="OHN28_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCQ2299343.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1203..2528
                     /locus_tag="OHN28_004046"
     CDS             1203..2528
                     /locus_tag="OHN28_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCQ2299344.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            2637..2873
                     /locus_tag="OHN28_004047"
     CDS             2637..2873
                     /locus_tag="OHN28_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2299345.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2885..3478
                     /gene="rclC"
                     /locus_tag="OHN28_004048"
     CDS             2885..3478
                     /gene="rclC"
                     /locus_tag="OHN28_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCQ2299346.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(3638..4507)
                     /locus_tag="OHN28_004049"
     CDS             complement(3638..4507)
                     /locus_tag="OHN28_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCQ2299347.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            4755..5612
                     /locus_tag="OHN28_004050"
     CDS             4755..5612
                     /locus_tag="OHN28_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2299348.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(5737..9984)
                     /gene="fdeC"
                     /locus_tag="OHN28_004051"
     CDS             complement(5737..9984)
                     /gene="fdeC"
                     /locus_tag="OHN28_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HCQ2299349.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         2616 a         2516 c         2279 g         2771 t
ORIGIN      
        1 gagctagagt ccatcaaaaa aatatataaa acccaaaacc accgttagaa atagaaatag
       61 ttgaaaggct cacaactccc ggaatgcgga ttatctttgc cctgatttct ggcggtttta
      121 ttcagggtgc aagctgtctc accctttccc gatattctcc cggagtacaa ccaaactcgc
      181 ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc tactgactca gcgatcacca
      241 caacagggag catttcccgg gaaaacatct gggccgctat ttgtagacgc aactttgtta
      301 atacagccag cggcgtggtt ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg
      361 acatgtgggc gatgctggcc agcgattcga cggtccaggc gtgtccaggc atttccatca
      421 tttgctgtat taccgcacca agacgtggat gcagaagcaa actgagaatg tttttctctg
      481 tattaacctg tgcaatccac tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc
      541 tacacagagc atccactccc ggcattgccg atctggattc ctgttgtaaa aacgggatcg
      601 cctccctcag ccagttatat tccacgctat ggttcactgg tgctacaaac agcgtttccg
      661 gcaaagacgt taaaaaataa cgcgccgaat gttgcagccg aagagttcca cagacaatac
      721 aggtcgattc attatcgaca tgactcaggc gatgagctga attttgtggt agcaggacca
      781 catttcccgg gcgtaatgta aaaatctctc ccgtcggcat ttccagcttc gccgctcctt
      841 gcgttaacgc atgccaacga ataaccgaca actccccggc accatgtgga agttgccagt
      901 cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac
      961 ggctaagagc atccatatca gactcctgag tggtttttgg attacaaaca ccaacaatct
     1021 tagtcaataa ttaatctaat accatcaaat agttaatcat gatttttgtt ttgtgattct
     1081 gattaagaaa aagaaaatac cagacgataa gacaaaatat caagcgttgt gaagaaatgt
     1141 tatttgctct tttgcgtcta tagtcatgat gtcaaatgaa cgcgtttcga caggaaatca
     1201 tcatgaataa atatcaggca gttattattg gttttggcaa ggctggaaaa acattagccg
     1261 tcacgctggc aaaagcaggt tggcgtgtgg ctctcatcga acaatcaaat gcaatgtatg
     1321 gcgggacctg tattaatatc ggctgtatcc caaccaaaac attggttcat gacgcacagc
     1381 agcacacaga ttttgtccgt gccatacagc gtaaaaatga agtggttaat tttttacgta
     1441 ataagaattt tcataatctt gcggatatgc ccaatatcga cgtgatcgac ggccaggcgg
     1501 agtttatcaa taatcatagc ctgcgtgttc atcggcctga gggaaatctg gaaattcatg
     1561 gcgagaaaat ttttattaat accggtgcac aaaccgtggt tccgccaatt cctggaatta
     1621 ccaccacgcc aggagtatat gacagcaccg gattacttaa tctaaaagaa ttgcctgggc
     1681 atttaggtat tttgggcggc ggatatattg gcgttgagtt cgcctctatg ttcgctaatt
     1741 ttggcagcaa agtaaccatt ttagaggcag cttcgctgtt tttgcctcgg gaagatcggg
     1801 atattgctga taatatcgcg acgattttac gcgatcaggg cgtcgatata atcctcaatg
     1861 cccatgtgga gcgaatcagt caccatgaaa atcaagtgca agtgcatagc gaacacgccc
     1921 aactggcggt ggatgcactg ttaatagctt ccggtcgtca accggctacc gcttcgttac
     1981 atccagaaaa tgcaggtatc gcagtaaacg aacgcggggc aattgtcgtt gacaagcaat
     2041 tacataccac cgcagacaat atttgggcga tgggagatgt taccggcggg ctgcaattta
     2101 cttatatttc actggatgat taccgcattg tgcgtgatga gttgctggga gaaggcagac
     2161 gtagtactga tgatcggaaa aatgtgcctt attccgtatt catgacaccg ccactgtcca
     2221 gggttggtat gacagaagaa caggccagag agagtggtgc tgatattcag gtggtgacat
     2281 tgcctgtagc tgcaattccg cgtgccagag tgatgaatga tactcgtggg gtattaaaag
     2341 cgattgttga taataaaacc caacgtatat taggggcatc actgctgtgt gttgactccc
     2401 acgagatgat caatatagtg aaaatggtga tggatgccgg gctgccttat agcatattac
     2461 gcgatcagat atttactcat ccgtcgatga gcgaatcact caatgatcta ttttcattag
     2521 tcaaataaac tcaaaatcag acgccagaac aaatattctg gcgtctcaga gaaaagaatc
     2581 ttattaattc cctgttactt tataaattct taatcacttc attgatggta ttttatatgt
     2641 ttaaaaaatc tgttttattt gcaacactat tatctggcgt tatggcattt tccaccaatg
     2701 cagatgataa aataattctg aaacatatca gcgtctcatc agtatcagca tcaccgacag
     2761 ttcttgagaa tgccattgct gatatggcca gaaaatataa tgcttcatcc tggaaagtta
     2821 catcgatgcg aattgataat aattcaaccg caacagcagt attgtataaa taaggatgtt
     2881 tacaatggaa agatacctgc acctgttaag tcggggagat aaaattggcc tgacattgat
     2941 tcgtctgagc attgcgattg tttttatgtg gattgggtta ttaaagtttg tcccttacga
     3001 ggcagacagc attacaccat tcgtcgcaaa cagtccacta atgtcgttct tttatgaaca
     3061 cccggaagac tataaacagt atctgactca cgaaggcgaa tacaaaccag aagcaagagc
     3121 atggcaaacg gctaataata cctatggttt ttccaacggt cttggcgtcg tggaggtgat
     3181 tattgctctg ctggttttgg ctaatcctgt caatcgctgg ttaggtttat tgggtgggct
     3241 gatggcattt accacgccgc tggtaacact ctcattttta atcaccactc cggaggcatg
     3301 ggtacccgca ttgggtgatg ctcatcatgg tttcccttat ttatccggtg ctggtcgtct
     3361 ggtattgaaa gatactctga tgctggcagg tgctgtcatg ataatggcag attcggcacg
     3421 ggatattctt aaacaacgca gtaatgaatc cagttcaacg ttaaaaactg aatattgata
     3481 atccgaacac tgtctgttgt tcactttttc aggcgtcagg ccccctttct gttgatgaag
     3541 cctgacgcca tattaccgga gatatgacag agtgacgatc aatatcactc ccctgttaaa
     3601 aaatttttgt cgtctcaggt aaaataagtg attaatatca atcatgtatt tttacagcaa
     3661 gacaaccacg aacaaactca ggattaaaat gttttaccga ttccccaaca taacccaaat
     3721 ctagcgaact aatttgcgcc atttctttct ctgtcagtga gaaatcccag atagcaaaat
     3781 tttcttcgat acgctgttga tgtgtcgatt tcggaataac gaccactccc cgttgaatat
     3841 tccaacgcag aatgacctga gaaatcgatt tttgatgggc atctgcaata ctctgaagca
     3901 gattattttc aaagggttta tgtcgtccac cacctaacgg agcccatgct tcaggctgca
     3961 cgttataata cttcatggtt gccagcgcct ctggttgggc aaaataggga tgcaactcga
     4021 cctggttgac catcggttta actcttaccg tttcacaaaa gttcgccaga acatgagggt
     4081 agaaattgga aaccccaatc gcttttaatt tgccctcttc ataggcatct tcgagtgcac
     4141 gccacgcact aaaataatcg cgcatggcct ggtgcaataa atagagatcg aagtattcta
     4201 gccccgattt ttttaatgat gcttcaatac ctgctgctgc aatatcctga ttcaacatat
     4261 cctgcaccca caatttagac gttataaata actcttctcg ggtacataaa ccttcagata
     4321 tggcttcacg aacggcttca ccgacggcat cttcattacc gtataccgct gcagtgtcga
     4381 tgagtcgata acccgtgcgg atagcattca acactgactg tttgcacacg tctttatcgg
     4441 taacctgaaa aacaccaaat cccatcatcg gcattttcag attgttactt aatacggaaa
     4501 attccactgt cagtccctcc attaggtatg aacaatgagt ctacgttaaa acgtaacctc
     4561 aaattagtat gtggattttg atatcactta tgcaaaaaat tcattaataa tgtaggactg
     4621 aaacctctct atttttgggg caatgaagca gacgctatca gtgcttttgc cttgggactt
     4681 gctgtttttg atacacttag ggcccaggac ataacgaaaa tgtgcgatat gacaaagaat
     4741 taacggagta tgtgatgatc aggcagaaga ttctacagca gctcctggag tggattgagt
     4801 gcaatcttga gcaccctatt tcaatcgaag atatcgcaca gaaatctggc tacagcagac
     4861 gcaacatcca gcttctgttc cgaaatttca tgcatgtgcc tttgggagaa tacattcgca
     4921 aacgaaggct ttgtcgtgcc gccattcttg tccggctctc cgcgaaatct atgcttgata
     4981 ttgcactctc tttgcatttt gattcacagc agtcattcag ccgcgaattt aaaaagttat
     5041 tcggctgctc tccccgtgaa taccgccacc gtgattattg ggatctcgca aatatcttcc
     5101 cttctttttt aatacgtcaa cagcaaaaga cggagtgtag attagtcaac tttcctgaga
     5161 cacctatttt tggcaattca tttaaatatg atattgaagt gtcgaataaa ttaccggatg
     5221 aagaagtcaa actgcgacgt catcatttag tcagatgtat gaagaatttt aagacggata
     5281 tctatttcgt ctccacgttc gaaccatcaa caaaatcggt cgatttgctc acagttgaaa
     5341 cttttgccgg tacggtatgc aaacaaaccg attcagagat gccgaaagag tggacaatta
     5401 accgaggact gtatgcctct ttccgctatg aaggtgagtg ggagcactac cctgaatggg
     5461 cgcgaaacct ctatctgatg gaattacctg ccagggggtt agccagagtg aacggcagtg
     5521 atattgagcg attttattac aatgaaaact tcgtcgaaat tgatagcaat aacattgttt
     5581 gcgaaatttt tatccccgtt cgcccggttt agcctgtaac tccatcacat aagatttacc
     5641 ctaccctgcc accgtctaat cggtgccagg gtaggtattc agtaaataat ggcgctgcgc
     5701 atgtcaacgc cattattatt tattacatcg tcgtttttat ttctcctcag caccttcagt
     5761 atctgcagga acggcgttaa ttgtcacagt cacgggtttc atttcatacc ctccatatgt
     5821 caaagagttg aatgtgaatg tgtatgtacc tggcttgttg gtactaaatg tacgcgtgat
     5881 tttcccgctc gcgaaatggt catttttcga cggcaggaac tgataatccg cctctgtcac
     5941 cccttccgga gcctcaatgt cgacccacat ggttcctcct accgggttgt ttgccgagaa
     6001 cagggttacc gtcagtgttg ctgtcgttac cccatccgcc accagagaat gcacatcaga
     6061 tgttgcggcg agctccgcgt ttttatcatc cgcgacgaat ttagattcgg cagttttcgt
     6121 cgattcctga ccgtcggcct gttccacttt cgccgtgagt gtatatgtcg ctgcgacagt
     6181 ggtcgtggcg gtgaaaatag cctgcccttg ctcattcgtt gtcgccgtcc cattgggagt
     6241 cagaactaaa ttttccgggc tggcagtcag cgtcacttcg ctatctttca gcaggttgtt
     6301 attggcatca gtcaccgtaa ctttatacgt ctgtttggtt ttgccgtccg ccactgcacg
     6361 gttgccaatc acctcaatac tggagatggt cgccgtagtc ttatcggcca caaacgttac
     6421 agactgcgca ttcgaaggct ggtcatttcc tgctgacgcc gtgatctcat aggttccggc
     6481 agtcacggaa accacctgca actccgcttt cccctcatcg ttggctttca cccacacatt
     6541 atcgcctgta agcggcttga caccccgggg cagattaaag gtcaccaggc tccctggaac
     6601 agggttacca tttttatcct caacgttcgc cgtcagcgta aaggcatctt tgccgtttgc
     6661 cactttttga acggcggtgt ctacctgcag gtttgcctga ccggtgctgg catccgcgtt
     6721 gaatttcacc gtgaccgtct tctgagcacc attcaccgaa gcggaaatat tgtgttctcc
     6781 cgcaaccgtt gacataagct caatgtccac tttccctgcc gcattggttg ttactgtatt
     6841 ccccgtcttg ctggtcacac cctgcggtaa agtcagcgta acttcttgcc cctgcaacgg
     6901 gttaccatag ctgtccacga cggtcagggt tatctggtta gcagactgtc cattagccag
     6961 ttggttatta accttcactg tcatatcacg aatctcagcc ttagatgcgt cacctgcaac
     7021 attcagcacc agtggctgag caacggcatt ttggccgttc actcgcggca tcacagacaa
     7081 ctgacccgcg gcagatccca gcgttaaggt cgacacgtag accccattac ctttctctgt
     7141 ccagtttcct gctgaaggac gctcactccc cgtgctggcg gcaccactaa acactggtgc
     7201 atctggcttc agcccggtga ccggattccc gtacgcatcc ttcacggtga aggtgagttc
     7261 cgtcgtcgtt ttaaccgttg gagttttatt gtccgcaaca agcgtagagt tcgctgatga
     7321 catctctccg gcaataacag tcagctgcgc ggcttcggtg gctgcaggct ggccgttgaa
     7381 gagcggcatg acacgaagct cgcccgtctt tccgcctgta gttaacgtag caacatagga
     7441 accgtcacct ttttcggtcc aactggaaac ggtcgcccct tcagaggcgg tccccgtcaa
     7501 acttgccgac aacgaaagac cactgatagc gttgccatgc gcatctttcg ccaccagcgt
     7561 tacggttgtg ctttcaccgg cttttacgtg atcttctgcg acagagactt tcgactggtt
     7621 tgaagataat gcatcagcca ccacggttac ttttgccgca ttcgctgccg cgtcctgccc
     7681 attgagcttc ggcataacgt ctaattcacc cgccgtagtg ccgagagaaa tctgcgcagt
     7741 ccaggtcccg tcgccattat ccgtccagcc tgatgccgta gaaccagcag cagctgcgcc
     7801 cgataatgac ggtgcatccg gattgaggcc agttacaggg ttgtcattag catcttttgc
     7861 cgtccagatt gccgtaactg taccgccaac caccggttta tcaggattca gggtgatgga
     7921 cgaatgtgct gcatcaagcg gcccggcaac aaacttcagc gtttgttgta acgtgcccag
     7981 ctgatactgc tcgctgaagg cacgcacaac aacgttcccg gcacgggtcg aagaaaccgt
     8041 ggcgctgtaa acccctggtt ttatttccga aatggcacca acggttacac cattagtgtc
     8101 ttgcggaaca agtcgcaggc ggctggcttc tcccgtcacc ggattaccct cggagtccac
     8161 cgctgtcagt gtcagcgtgt aggcttgctg accatcagca accacatcac ctgacggctc
     8221 gttagcgctc agggtggagt ttgccacatc catcatcgtg gcccgcagtt ctgcagtgac
     8281 agttttgctc atgtcatcaa cgctaacagt aatcgttgcc tcacctgact gcgttccggt
     8341 agtaaagaca gactgataca cccctgattc agtttcggtg aactcaccca gtgttggctt
     8401 tgcctgtgat ttagtggcct tcagggtacg agtcacaata tttccagccg gtttgaaggt
     8461 tagttcagtc ttgatctgat ctttcatgcc cgtgactggc tgaccctcgg cgtcgcgcag
     8521 agacagcacc agcggctttt gctcattacc gttagcaagc atttgaatac ggctctgacc
     8581 gtcaagcgtt aacgccgtac gatcggcgct cataccagct ccgctaataa ccacttctgt
     8641 ctgcacacgt ttcgaggcat tgcctttgtt atcgtaggca atcgctgaaa tcgcataata
     8701 attgtctttg cctgcctgat aagccgggag cgtcacttgc cactgattgc cctgccccgt
     8761 aatttttccg cctgcggcca gcaaagacgg cgcttcccat tgcacatttt tcagaccgtg
     8821 agttgctttg ctgaccacaa gccccaggga aaccgtctgg ccgcccttgc cttcaatacg
     8881 ctcaggcaga gcaatacgga tcacttcaga tttgcgatac tcaagaacga tattgttatt
     8941 acgctcaacc aggtcatagc ggctgcctgc cagcattcga cgctcgcgaa tgctgtctgt
     9001 atcgagttgt ttttccagag gttcgccaat ccgataatta acttccaggc caaagcgagt
     9061 gtcattctca ccactcttgc cctgcttatg cccggcactc agggtcagaa gaggcactgg
     9121 cgtgtaattc acttcagcgg taatcgcatg tggatctttc tggcgtttat ctttaccaaa
     9181 cagcccgact tcatcgccat aatactgttc atacatgagg cttgcgccaa gctgcggcca
     9241 ggcaggtaaa tagccctcag cacgaatatc ccagccattc gccgggcgtt cctgataatc
     9301 ctcaacatcc ggcgattttt tccagccaga agcccggata taaccattgg cgctcagttt
     9361 caaataatca cgccagtatt ccgcaccaac accaatgcgg gtatgactac gggataaatc
     9421 atgatcgata aaagtattca cccccgccat ccagtcattt tctgaaaaat gacgccagcc
     9481 aaaaccaata tttgactgag tacgatcgtc ggtacgatgt attgccccct gagtgaacaa
     9541 catatttgtc ggtgtatcat aaatcggata aagcatttcc agcgaagagt ccttcagcga
     9601 gaaattttta tcgacattca gtttgacgcg cgcagtaccg tatttcccga gccactcctg
     9661 aatttcctgg ttagctttag cggtggccat tccggtaata aagttacgtg tcgcatcgct
     9721 atctggctga ctgcttaaaa atgtcccggc attagcggca agcgacgcga catttttctc
     9781 cacgttatta ttagcagtta ccgtagtatt ttccatgctc aaccgtggct gaaccgcatg
     9841 ctgcgcacgt gctgccatta ctggcgtaaa ggtgactgcg agtggaaaaa gaacctgaac
     9901 agagatattt gcccacgcca cgcagcgggc cagaactgaa taacgaaatc gtggctgttt
     9961 attgtctgtt ttataacgtg acattgagat aatttcctga tgaacgaatt aacgaacata
    10021 ggcagacaca aataatggcg tctgcttgtc agaaaaaccg gggatttccc ggtttccttt
    10081 tccacagaaa aggaaatacc tttatgtaag aaagtacacc gccacctaat atatccagtg
    10141 tctgtagcca ctcatcaggt ggttgggcgc gggattatat tt
//
LOCUS       DAJOTB010000159        10008 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJOTB010000159 DAJOTB010000000
VERSION     DAJOTB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.159"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(83..1240)
                     /gene="intS"
                     /locus_tag="OHN28_004052"
     CDS             complement(83..1240)
                     /gene="intS"
                     /locus_tag="OHN28_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCQ2299350.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1402..1476)
                     /locus_tag="OHN28_004053"
     tRNA            complement(1402..1476)
                     /locus_tag="OHN28_004053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Arg,seq:cct)
     gene            complement(1552..2484)
                     /locus_tag="OHN28_004054"
     CDS             complement(1552..2484)
                     /locus_tag="OHN28_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCQ2299351.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2778..3533
                     /gene="mlaA"
                     /locus_tag="OHN28_004055"
     CDS             2778..3533
                     /gene="mlaA"
                     /locus_tag="OHN28_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCQ2299352.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3595..4935)
                     /gene="fadL"
                     /locus_tag="OHN28_004056"
     CDS             complement(3595..4935)
                     /gene="fadL"
                     /locus_tag="OHN28_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCQ2299353.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            5307..5591
                     /locus_tag="OHN28_004057"
     CDS             5307..5591
                     /locus_tag="OHN28_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCQ2299354.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5772..7082
                     /gene="fadI"
                     /locus_tag="OHN28_004058"
     CDS             5772..7082
                     /gene="fadI"
                     /locus_tag="OHN28_004058"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCQ2299355.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7082..9226
                     /gene="fadJ"
                     /locus_tag="OHN28_004059"
     CDS             7082..9226
                     /gene="fadJ"
                     /locus_tag="OHN28_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCQ2299356.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            9429..9914
                     /gene="sixA"
                     /locus_tag="OHN28_004060"
     CDS             9429..9914
                     /gene="sixA"
                     /locus_tag="OHN28_004060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCQ2299357.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2423 a         2409 c         2568 g         2608 t
ORIGIN      
        1 gtgaagtaac catgtaaaat gattgtttca tagcctatga gacacacaag gctttgtgct
       61 cttcgatagt tgttaaggcg gatcactcta ccttctcatc aagccaatcc gcccaccact
      121 gcatcatttc tctgcgctta tcgagatact gagcatggtt gtaaataccg cgcacagatc
      181 cgccgttggc atgtgccagt tgcacttcaa tagcgtcagc aggccattcg tgctcgttca
      241 taatcgtgct gaattcatgc ctgaatccgt gaccgctttc tagaccctca tagccgattt
      301 gtttgatcac aagtagaacc gcgttctcgc agattggctt cttcttatcg ttgcgcccgg
      361 caaaaacaaa ctctgatact ggtttggtga tggagcttag cgtagtgaga agttcaacca
      421 cctggtctga catcgggacc acatgaattt tgcgtccctt catcacactg gcgtcgatgg
      481 tgataatcct gttttcaaaa tcgacgttct tccatagcat ggaacgaagc tctttcgttc
      541 ttagggctgt gtagcgtaaa actttggtcg caatgagcga tacgatactt cctgaaaatg
      601 ttgccagtgc tttgttgaat gcagggatct ggtctgcagg aagaaacggg aagttcttct
      661 tgcggtatcc cttcatggcg tcagcaaggt caggtgccgg gttatattta gccctaccag
      721 tgacaatagc gtaacggaaa acctcgccgc atcttctgcg ggctttgttg gctcgctcca
      781 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
      841 gaatttcaag gccgccgatg ataggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      901 ttgcatagcc tactgaccag acttgcttct tgtgctcgta ccattccttg taaatcgcac
      961 taaaggaatt gttgttagac gaagcctttt tcgcttttac cggatcgatg ccaaccgaga
     1021 tgtctttcct cgcggtccat gctttatctc ttgcctcctg caaagtcata agtggatatt
     1081 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
     1141 tccctgatac agggacataa aggtacaggc cattaccatc gagtaggcgg tatggttttt
     1201 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
     1261 aaattatttt atccactttt tacccgtcat gtagtgcggc tgtcaacgat ctgaagcgaa
     1321 ccatgacgaa ctgtgaatct acggaaggct tgatattcag gggattttgc ggactggtac
     1381 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
     1441 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta
     1501 atggactacg gggtaagtac acagccgctc gattgcttaa accctcgcca tttatgccgg
     1561 gtttttatca tttttcttaa tgttttccgc acgttctgct ttttgacgtg cttctgcttt
     1621 gcgcttgttg ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt
     1681 gccgccgcag atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca
     1741 gtgcagcgtg ccgttaaaca ccagatagag gatttcaaca gaaccgacaa cgatgtgggt
     1801 ggtgtcaccc agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc
     1861 cgctgcagga aacatccata ccatagtggc gatcagccag ccggaaataa tcgcgttggc
     1921 aaacatctcg ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg
     1981 agtttcttca ttgaagatag gcatatattc aaatgcccac gcagcaatac ctgtcccgag
     2041 aatattaccc agcagcacga tgccccataa ccgcataagt aagccgacgt tgctcattgt
     2101 cggtttttgc atgacgggta gtaccgcagt tacggtgttt tcggtaaata attgctggcg
     2161 ggccattatg acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc
     2221 cggcacgcct tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga
     2281 caggcccgcc gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc
     2341 accatcctgg cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc
     2401 tatttctatt tttttaccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg
     2461 ttgatcaatt ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc
     2521 gtagcggctt ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggccgcg
     2581 tttgcctttt tctgtttcta ttgaatcaag tagcctacag ggcggcgatt accaggctat
     2641 gatcaaatca gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac
     2701 acctaaacag gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa
     2761 aaaacaggga gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc
     2821 tggtggggtg tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt
     2881 ttaaccgcac tatgtacaac ttcaacttca atgtattaga cccgtatatt gttcgaccgg
     2941 tcgctgtcgc ctggcgtgat tatgttccgc aacctgcgcg taacggtttg agcaacttta
     3001 ccggcaacct tgaagaacct gcggtaatgg ttaactactt cttgcagggc gacccttatc
     3061 aggggatggt ccactttacc cgctttttcc tgaataccat tttgggaatg ggcggcttta
     3121 ttgatgttgc agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta
     3181 gtacgcttgg tcattatggt gtgggttatg ggccgtacgt tcagttaccg ttctacggta
     3241 gcttcacgct gcgtgatgac ggtggtgata tggcggatag tctttacccg gttctttcct
     3301 ggctgacctg gccgatgtct gtgggtaaat ggacgcttga agggatagaa actcgtgcgc
     3361 agttgttgga ttccgacggt ctgctgcgtc agtcctccga tccttatatt atggtgcgtg
     3421 aagcgtactt ccagcgtcat gatttcatcg ctaatggcgg tgaactcaaa ccgcaggaaa
     3481 atccgaacgc gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa
     3541 acaaaaaagg tgagtcgcaa aactcacctt ttttcttatt aagctaacgc gttatcagaa
     3601 cgcgtagtta aagttagtac cgaacagcca ggctttacct tcagactcga aggaataagc
     3661 tgccgctcct tcaccttcgg tgaatttcac tttctgacca tgcatataag atgcgccaac
     3721 atcaatggat gcatcttcat taaatgcgta ggttaaccca gcacttaacc acaaacgatc
     3781 ctggtcagga atggaaatag aacgtttatc tgccggaacc gggctgtcgt cataagcgat
     3841 accggtacgg aaggtccagt ttttatcata atagtaggtt gtacccaacg cgatacgata
     3901 cgcgtcccgg aatccttcat ctttatagaa cagagtctga ccactatcgc ctttcgcttt
     3961 caattcctgg aactggctcc aactggtata ggccatgctg tagtgaattg cccactgcgg
     4021 cgcaacacgg ttataaccag aaatctccca catctcaggc aggttcagcg ttaaggatcc
     4081 attttgggtt gcccccatcg tgccatacgg taagcccatg ttacccaaaa tattgttcag
     4141 atttgggtta atggagcttt tgtaatcccc atcgaagtcg attttaactt ctgaacgata
     4201 tgtcagcccc caacggttat ctttatccag ttcataaaga atacccgcgt tccagccaaa
     4261 gccccattcg tcacctttca aatgagcaat ttgagagtcc ggagtattaa taccattggt
     4321 taatgaagcg ggtacaccct gggcaatcaa ctgttgagac aacaatggcc ccagcgaacc
     4381 ggcgtaacgc tcaatttttg ccttggcata aactgcatca aaaccaagac caaaactcca
     4441 gctattgtta aggcgataag ccccgctaag gttaaggttg attgtttgca gatcggtttt
     4501 accgccatag ataccagcag cgtagttgtc ggtaaattcc gtagccaggc catagttgga
     4561 agttattgac gctccccaac caaactgatc gttaataggc gcgacaaagt gcaggttagg
     4621 tacccacgct gtcggcgcga tatttttagc atttgcactc tgaccggtta aattagaatg
     4681 tccggtaata tctacatccg gatcaatata aaccgcaccc gcagaaaatg tcgggcggtc
     4741 aaacatggta atcaatgcgg ggttacggct aacgttacct gcatcatcgg caattgcacc
     4801 ttcccctgaa taagcccggc ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc
     4861 cgaccaggcc tgggtggaga taagtgccac tgcgactgcg agagcagact ttgtaaacag
     4921 ggttttctgg ctcatgacca taacctcaat gatttatttt tatacaaact atgttacgtg
     4981 ctgtaacagg agcgcgaagt gtagggtctg aggtacatct tagaaatcag accagtggcg
     5041 agagtatagg tcggaccagc tggggtgttg caagtatgtt tctatctttt ttcagatatg
     5101 atctaagaaa cgcgatccag ctggcaaaat tgggaggtgg cgcactcatg gattcgggtg
     5161 aattttgttt tagatcattt ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt
     5221 aacaactgga gcagcacttt ccggaagcgt aaaatatcca caacgtttat ctggcacctt
     5281 gggtgcacat acagaggaaa tcaacgatga gtaaatgtag tgctgatgaa accccggttt
     5341 gctgctgtat ggatgttggc accattatgg acaactccga ctgcaccgcg tcttacagcc
     5401 gtgtgtttgc aaaccgcgca gaagcagaac aaacgctggc ggcgctgact gaaaaagccc
     5461 gtagcgtgga atccgaaccg tgcaaaatca ccccgacttt cactgaagag tctgacggtg
     5521 tgcgcctgga tatcgatttc acttttgcct gcgaagctga aatgctgatc ttccagctgg
     5581 gtctgcgtta attcctctcg ggtacatacg cccctgtcag ctgctgacag gggcgtttta
     5641 ttttctcctc ctcttttctc tctgtgtcat agctcccact ttcccttttc tccacttggc
     5701 taaatgtaaa aaaatggtta agactatgat caggtcagac cactttattt atttttttac
     5761 aggggagtgt tatgggtcag gttttaccgc tggttacccg ccagggcgat cgtatcgcca
     5821 ttgttagcgg tttacgtacg ccttttgccc gacaggcgac ggcttttcat ggcattcccg
     5881 cggttgattt agggaagatg gtggtaggcg aactgctggc acgcaccgag atccccgctg
     5941 aagtgattga acaattggtc ttcggtcagg ttgtgcaaat gccggaagcg cccaacattg
     6001 cccgtgaaat tgttcttggc actggaatga atgtgcatac tgatgcttac agcgtcagcc
     6061 gcgcttgcgc caccagtttt caggcggttg cgaacgttgc cgaaagcctg atggcgggaa
     6121 ctattcgagc ggggattgcc ggtggggcag attcctcttc cgtattgcca attggcgtca
     6181 gtaaaaaact ggcgcgcgta ctggttgatg tcaacaaagc ccgcacaatg agccagcgcc
     6241 tgaaactctt ctctcgcctg cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag
     6301 aatattctac cggcttgcgg atgggggaca ccgcagagca aatggcgaaa acctacggca
     6361 tcacccgaga acagcaagat gcactggcgc accgttcgca tcagcgtgcc gctcaggcgt
     6421 ggtcagaagg taaactcaaa gaagaggtga tgactgcctt tatccctcct tataaacaac
     6481 cgcttgtcga agacaacaat attcgcggta attcctctct tgctgattac gcaaagttgc
     6541 gtccggcgtt tgatcgtaaa cacgggacgg taacggcggc gaacagtacg ccgctgaccg
     6601 atggcgcggc agcggtgatc ctgatgactg aatcccgggc gaaagaatta gggctggtac
     6661 cgctgggcta tctgcgcagc tacgcattta ctgcgataga tgtctggcag gacatgttgc
     6721 tcggtccagc ctggtcaaca ccgctggcgc tggaacgtgc cggattgacg atgggcgatc
     6781 tgacattgat cgatatgcac gaagcctttg ccgcccagac gctggccaat attcagttgc
     6841 tgggtagcga acgttttgct cgcgacgtac tggggcgtgc acatgccact ggcgaagtgg
     6901 acgagagcaa atttaacgtg cttggcggtt cgattgctta cgggcatccc ttcgcagcga
     6961 ccggcgcgcg gatgattacc cagacattgc atgaacttcg ccgtcgtggc ggtggatttg
     7021 gtttagtgac cgcctgtgct gcgggtgggc ttggcgcagc aatggttctg gaggcggaat
     7081 aatggaaatg gcatcagcgt ttactcttaa tgttcgtctg gacaacattg ctatcatcac
     7141 catcgacgta ccgggtgaga aaatgaatac cctgaaggcg gaatttgcct cgcaggtacg
     7201 cgccattatt aagcaactcc gtgaaaacaa agagttgcgt ggcgtggtgt ttgtctccgc
     7261 gaaaccagac aacttcattg ctggcgcaga catcaacatg atcggcaact gcaaaacggc
     7321 gcaagaagcg gaagtactgg cgcggcaggg gcaacagtta atggcggaga ttcatgcttt
     7381 gcccattccg gttatcgcgg ctattcatgg cgcttgcctg ggcggcgggc tggagttggc
     7441 gctggcgtgc cacggtcgtg tttgtactga cgatcctaaa acggtgctcg gtttgcctga
     7501 agtacaactt ggattgttac ccggttcagg aggtacccag cgtttaccgc gcctgatagg
     7561 tgtcagcaca gcattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcggt
     7621 aaagctgggg ttggtggatg acgtcgttcc gcactccata ttgctggaag ccgctgttga
     7681 gctggcaaag caggatcgcc catcttcacg ccctctacct gttcgcgagc gtattctggc
     7741 ggggccgtta ggtcgtgcgc tgctgttcaa aatggtcggc aagaaaacag aacacaaaac
     7801 gcaaggtaac tatccggcga cagaacgcat cctggaggtt gtcgaaacgg gattagcgca
     7861 ggggaccagc agtggctatg acgccgaagc tcgggcgttt ggtgaactgg cgatgacgcc
     7921 gcaatcgcag gcgctgcgca atatcttttt tgccagtacg gacgtgaaaa aagatcccgg
     7981 cagtgatgcg ccgcctgcgc cattaaacag cgtggggatt ctaggtggtg gcttgatggg
     8041 cggcggtatt gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat
     8101 caacccgcag ggcataaatc atgcgctgaa gttcagttgg gatcagcttg agggcaaagt
     8161 tcgccgtcgt catctcaaag ccagcgaacg tgacaaacag ctggcattaa tctccggaac
     8221 gacggactat tgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct
     8281 cgaattaaaa caacagatgg tggcggaagt ggagcaaaat tgcgctactc ataccatctt
     8341 tgcttcgaac acgtcatctt taccgattgg cgatattgcc gctcacgcca cgcgacctga
     8401 gcaagttatt ggcctgcatt tcttcagtcc ggtggaaaaa atgccgctgg tggagattat
     8461 tcctcatgcg agtacatcgg cgcaaaccat cgccaccacg gtaaaactgg cgaaaaaaca
     8521 gggcaaaatg ccaattgtcg tgcgtgacaa agctgggttt tacgtcaatc gcatcttagc
     8581 gccttacatc aatgaagcta tccgcatgtt gaccgaaggc gagcgtattg agcatatcga
     8641 tgccgcgcta gtgaaatttg gttttccggt aggcccaatc caacttttgg atgaggtagg
     8701 aatcgacaca gggactaaaa ttatgcctgt actggaagcc gcttatggag aacgttttag
     8761 cgcgtctgca aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg
     8821 tcggggtttc tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc
     8881 catttacccg ctcattggcg tacaagggca ggggcgactc tccgcaccgc aggttgctga
     8941 acggtgtgtg atgttgatgc tgaatgaagc agtacgttgt ctggacgagc aggttatccg
     9001 tagcgtgcgt gacggggata ttggtgcggt atttggcatt ggttttccgc catttctcgg
     9061 tggaccgttc cgctatatcg attctctcgg cgcaggcgaa gtggttgcaa taatgcaacg
     9121 actggccacg cagtatggtt cccgttttac cccttgcgat cgtttagttg agatgagcga
     9181 gcgtggggaa agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagtta
     9241 tatgaatccg cgctgaatgg cggagtgttg ctcaaaatgt aaacgcatat tgactatact
     9301 tacgccattg aggtaaaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc
     9361 caccgttttc tttctctggt ttcagatgaa agaaaacggg cgaatctggt taacaaaagc
     9421 ggtgcaatat gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg
     9481 attcggttcg tcctctgacc actcatggtt gtgacgaatc tcgcctgatg gcgaactggc
     9541 tgaaaggtca aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc
     9601 aaacacttga agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg
     9661 agttaacgcc ctgcggcgat gttggtctgg tcggtgccta tttgcaggcg ttgaccaatg
     9721 aaggtgttgc ttcggtactg gtgatctccc acttaccgtt agttgggtat ctggttgccg
     9781 agttatgtcc gggcgaaacg ccgcctatgt ttaccacgtc agcgattgcc agcgtaacgc
     9841 ttgatgaaag cggaaaaggc caattcaact ggcagatgag cccttgtaat ttgaagatgg
     9901 caaaagcaat ttaagcaatc aatgcccggt tacgtgcgcg tacaccgggc agtttctccg
     9961 tcatacaatc attctttttt tgtctaaatg ttgttaatat ttaacaac
//
LOCUS       DAJOTB010000160        10006 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJOTB010000160 DAJOTB010000000
VERSION     DAJOTB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.160"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            248..1009
                     /locus_tag="OHN28_004061"
     CDS             248..1009
                     /locus_tag="OHN28_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299358.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            1030..2523
                     /locus_tag="OHN28_004062"
     CDS             1030..2523
                     /locus_tag="OHN28_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCQ2299359.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            2652..3911
                     /locus_tag="OHN28_004063"
     CDS             2652..3911
                     /locus_tag="OHN28_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCQ2299360.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            4146..5312
                     /gene="nhaA"
                     /locus_tag="OHN28_004064"
     CDS             4146..5312
                     /gene="nhaA"
                     /locus_tag="OHN28_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCQ2299361.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5378..6277
                     /gene="nhaR"
                     /locus_tag="OHN28_004065"
     CDS             5378..6277
                     /gene="nhaR"
                     /locus_tag="OHN28_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCQ2299362.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(6335..8374)
                     /locus_tag="OHN28_004066"
     CDS             complement(6335..8374)
                     /locus_tag="OHN28_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCQ2299363.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYNYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(8402..9775)
                     /locus_tag="OHN28_004067"
     CDS             complement(8402..9775)
                     /locus_tag="OHN28_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2299364.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
BASE COUNT         2672 a         2218 c         2389 g         2727 t
ORIGIN      
        1 atacgttcag cagccaatgc taagttcatc atgaaaaatg cctataaatc aaagggtgat
       61 atttaaaaca gacacatttt ctgaaacacg atatccctcc gcccgttcgc aaaagtaatc
      121 tgcgatcttc atcacgcttc gatagtcatc tttaaatata ctggcctggt cattcttaat
      181 aaaaatgaat tgtctggtgt attaattcct atggaatcat aaaaacaatg aatggagaac
      241 atattatatg tctgccaaaa gacgacttct tattgcgtgt accctgataa cagctatcta
      301 tcattttcct gcatattctt cattagaata taaaggaacc tttggttcaa taaatgcggg
      361 ttatgcagac tggaatagtg gatttgtaaa cactcaccgt ggtgaagtat ggaaagtgac
      421 tgcggatttt ggggtaaatt ttaaagaagc agaattttac tcattttatg aaagtaatgt
      481 actcaatcat gctgtagcag ggcgaaatca tacggtttca gtaatgacgc atgtcagact
      541 ctttgactct gatatgacat tctttggcaa aatttatggc caatgggata actcatgggg
      601 tgacgatctg gacatgtttt atggattcgg ttacctcggc tggaacggcg agtggggctt
      661 ttttaaaccg tatattggat tacataatca atctggtgac tacgtatcag ctaaatatgg
      721 tcaaacgaat ggttggaatg gttatgttgt tggctggaca gcagtattac catttacgtt
      781 atttgacgaa aaatttgttt tatctaactg gaatgaaata gaactggaca ggaacgatgc
      841 ttacacggag cagcaatttg gccggaacgg gttaaatggc ggtttaacta ttgcctggaa
      901 gttctatcct cgctggaaag ccagtgtgac gtggcgttat ttcgataata agctgggcta
      961 cgatggcttt ggcgatcaaa tgatttatat gcttggttat gatttctaat tttttatcag
     1021 ggaataatta tgcagaaaac gttaatggcc agtttgatcg gccttgcagt ttgcacaggg
     1081 aatgctttta gtcctgcctt agccgcagag gctaaacaac ctaatttagt cattattatg
     1141 gcggatgatt taggttatgg cgatttagca acatatggtc atcagatcgt taaaacacct
     1201 aatatcgaca ggcttgccca ggaaggggtc aaatttactg actactatgc ccccgctcct
     1261 ttaagttcac cttcacgcgc agggctatta accggccgga tgccatttcg tactggaatt
     1321 cgctcatgga ttccttcagg caaagatgtt gccttagggc gtaacgaact cacgattgct
     1381 aatctactca aagcgcaagg gtacgacacg gcaatgatgg gtaagctgca tctgaatgca
     1441 ggcagcgatc gcaccgatca gccacaagca caagatatgg gctttgatta ctcactggtt
     1501 aatacggcgg gctttgttac cgacgccacg ctggataacg ctaaagaacg cccgcgttat
     1561 ggcatggttt acccgacagg ctggctacgt aacgggcaac ccactccacg agccgataaa
     1621 atgagcggtg agtatgtcag ttcggaagtc gtcaactggc tggataacaa aaaggacagc
     1681 aagcctttct tcctctatgt tgcttttacc gaagtgcata gccccctggc ttcgcccaaa
     1741 aaatacctcg atatgtactc acaatatatg agcgcgtatc agaagcagca tcctgattta
     1801 ttttatggcg actgggcaga caaaccctgg cgtggtgtgg gggaatatta tgccaatatc
     1861 agctatctgg atgcacaggt tggaaaagtg ctggataaaa tcaaagctgt gggtgaagaa
     1921 gataacacaa tcgttatttt taccagtgat aacggtccgg taacgcgtga agcgcgcaaa
     1981 gtgtatgagc tgaatttggc aggggaaacg gatggattac gcggtcgcaa ggataacctt
     2041 tgggaaggcg gaattcgtgt tccagccatt attaaatatg gtaaacatct accacaggga
     2101 atggtttcag atacacccgt ttatggtctg gactggatgc ctactttagc gaaaatgatg
     2161 aacttcaaat tacctacaga ccgtactttc gatggtgaat cgctggttcc tgttcttgag
     2221 caaaaagcat tgaaacgcga aaagccatta attttcggga ttgatatgcc attccaggat
     2281 gatccaaccg atgaatgggc gatccgtgat ggtgactgga agatgattat cgatcgcaat
     2341 aataaaccga aatatctcta caatctgaaa tctgatcgtt atgaaacact taatctgatc
     2401 ggtaaaaaac cagatattga aaaacagatg tatggtaagt ttttaaaata taaaactgat
     2461 attgataatg attctctaat gaaagccaga ggtgataaac cagaagcggt gacctggggc
     2521 taacctgata atcataataa atgtaatgta tgccatttag ggctatgccc gcctgattca
     2581 gtgctacgca ttgaatcagg ttcttttcgc gttgaacaat atcaagatac ggctattcgc
     2641 agggagtgga tatgtttacc aacgtaaatg ttgattgttg caaaacacca ggatgtaaaa
     2701 acctggggtt gctgaatagc caggattatg tcgcacaggg taaaaatatt ttatgccgtg
     2761 aatgtggtta cttgtttcca gtgatatctg aacagtcgct taatatttat cgtaatattg
     2821 tgaatcactc ctggagaggt ttgatttgcc aatgttcaac ttgcggaggc acgtccctca
     2881 aaaaatatgg atattctgca caaggccaga gaagaatgta ttgccatcat tgtgagaaaa
     2941 catttatcac tctggaacat gtaattacca caccacgagg agcccagtta gcattgatga
     3001 ttgagcaagg ggaggcactt gcggatatca gaaagtcatt acttcttaac agcactggac
     3061 ttagccgtga actgttaaaa ttagcgcgtg aagcaaacta taaagaaagt cgacagtgtt
     3121 tcccagcttc tgatattacc ctgagtaccc gcgcttttcg cgtcaagtat aatggtagca
     3181 ataactctct ttatgctctt gttaccgcag aagaacaaag cggcagggtg gttgccatct
     3241 caaccaatta ctccccatct gccgtagagc aacattatca atacacatcg aactatgaag
     3301 agcgtatgtc tccagggacg ctggcacatc atgtccagcg caaagagtta cttactatgc
     3361 ggcgggatac cttgtttgat attgattacg gcccggcagt tttacatcaa aacgatccgg
     3421 gaatgttggt aaaaccggtt cttccggcat atcgtcattt tgaactggtc agaatactga
     3481 ccgatgagca ttccaacaac gttcagcatt accttgatca cgaatgcttt atattgggcg
     3541 gctgcctgat ggctaatttg cagcatattc atcaaggtcg ctgccatatt tcctttgtca
     3601 aagagcgcgg tgtggcaccc gccaccattg attttccgcc gcgattattc cttagtggtg
     3661 gggtacgaaa taatgtctgg cgtgcatttt ctaaccgcaa ttattcaatg gctgtatgca
     3721 atctcactgg cagtaagaaa gtccgcgaga tgcggcatgc aacattgaac agtgcgacgc
     3781 gttttatcca ctttgtggag aaccatcctt tccttatatc attgaaccga atgtctcctg
     3841 cgaatgtcgt ttctacatta gatatcctca aacatctgtg gaataaaaaa ctagagcatg
     3901 gaacaattta attattactt ttccgaataa tttcttcaga aatcttgaca ttagactatg
     3961 attaatgggg tttaaggtca tcagccgccg ctttcgggca tcaaaaactc atctctcgct
     4021 gatggcgcaa ttcttttaat agatcgtaaa aagcgagcta ttcctacact ataatctgat
     4081 tttaacgatg attcgtgcgg ggtaaaatcg tgaaaacgat ctattcacct gaagagaaat
     4141 aaaaagtgaa acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctca
     4201 ttattgccgc tgtattagcg atgattatgg ccaacagcgg tgcaaccagt ggatggtatc
     4261 acgactttct tgagacgccg gttcagctcc gggttgggac acttgagatc aacaagaaca
     4321 tgctgctatg gatcaatgac gctctgatgg cggtattttt cctgttggtt ggtctggaag
     4381 ttaaacgcga gctgatgcaa ggttcgctgg ccagtctgcg ccaggcggca tttcctgtta
     4441 ttgccgcaat cggcgggatg attgtcccgg cattgctcta tctggctttt aactatgccg
     4501 atcctatcac tcgcgaaggc tgggcaatcc cggcggcgac tgacattgcc tttgcacttg
     4561 gtgtgttggc gctgttggga agtcgtgttc cgttagcgct gaagatcttt ttgatggctc
     4621 tggctattat cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact
     4681 tatcgatggc ctctcttggc gtcgcggctg tagcaattgc ggtactcgcg gtattgaatc
     4741 tgtgtggtgt acgccgcacg ggcgtttata ttctggttgg cgtggtgctg tggacagcgg
     4801 tgttgaaatc aggtgtccat gcaacgctgg ctggcgtcat tgtcggcttc tttattccgt
     4861 taaaagagaa gcatgggcgc tctccggcta aacgtctgga gcatgttttg catccatggg
     4921 tggcgtatct gattttgccg ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggtg
     4981 tcacgctgga aggtttgacc tccattctgc cattagggat catcgctggt ttgctgattg
     5041 gcaagccact gggtattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcacatc
     5101 tgccagaggg aacgacttac cagcaaatta tggcggttgg tatcctgtgc ggtatcggtt
     5161 ttactatgtc tatctttatt gccagcctgg catttggtag cgtagatcca gaactgatta
     5221 actgggcaaa attaggtatc cttgtcggtt caatttcttc ggcggtaatt ggatatagct
     5281 ggttacgcgt tcgtttacgt ccatcagttt gacaggacga tgtactgggg agccataaac
     5341 ggctcccttt tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca
     5401 cttgtattac ttctggcatg tctacaaaga aggttctgtg gttggcgcag cggaggcgct
     5461 ttatttaaca ccacaaacca ttaccgggca gatccgggcg ctggaagagc gcctgcaagg
     5521 gaaactattt aagcgtaaag gacgtggtct ggaacccagc gaactggggg aactggtcta
     5581 tcgctatgcc gataaaatgt tcaccttaag ccaggaaatg ctggatatcg tcaactatcg
     5641 caaagagtcc aacttattgt ttgatgttgg tgtggcagat gcactttcca aacgtctggt
     5701 cagtagcgta cttaacgccg cagtggtaga aggcgagccg attcatcttc gctgcttcga
     5761 atccacccac gaaatgctgc tggagcaatt aagtcagcat aaactggata tgatcatttc
     5821 cgactgtccg atagattcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg
     5881 tggcgtgagt ttctggtgta caaatccacc accggaaaaa ccgttcccgg cttgtctgga
     5941 agaacggcgg cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg
     6001 gtttaactcc cagggattaa acgtagaaat ccttggcgag tttgatgatg ccgcgttgat
     6061 gaaagctttt ggtgcgatgc acaatgcaat cttcgttgct ccaacgcttt atgcatatga
     6121 cttttatgcc gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta
     6181 ccatgctatt tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac
     6241 ggattattct tcgttgtttt ctcccgcctc acgatagcta ttaaaactca cgccccgcta
     6301 catacggggc gtgctaatga acaaacgatg tcctttaaac gggaaatatc tggcgatatt
     6361 tccctttctc gcgaatgaat accggtatct ggtgcagcgg tgcgcaatga tgaagcgttt
     6421 gcccacccgc gtagtgttca tgggtataaa aatcgatcca tgtctctcca gcaggcaacc
     6481 agacatcacg tgaacgtgta ccttcgtgca ttacaggggc aacgagaagg tctgggccaa
     6541 agcagtactg atcataaata ttccagcttt ctgcctggtc aggaaattca aagaacatcg
     6601 tgcgcattat cgggctgttc ttctcgtgcg tctcagccat aattttactg atataagatt
     6661 tcagtttttc acgcaactgc aggcaggaag tgagaatttc accggtttct tcaccatagc
     6721 tccagacttc attcggcgcg ccagtcggac attgtgctat gccatcacga tagggttgtt
     6781 caggcaaaac ctgcgggtcg cggttgccat gtaaacgcat tactgggctg aatacccccc
     6841 actggaacca gcgaattaac agttcatgga atttcggatc gtgaatatta ccgccgtgga
     6901 aaccaccaat atcggttgtc caccacggga tcccggcaat tcccatgttc aagccagcgg
     6961 caaactgatt acgcaaagaa cggaaagagg agtgaatatc gcctgaccac accagagcgc
     7021 catatttctg actgccagcc caggcacagc gcagcaggtt aatcacctgc ttttcaccag
     7081 ccgcggtcat accatcgaag aacgttttgg cgtacatacg cggatagata ttaccgactt
     7141 ccagcacagg ccctgcgtag taacgatagt tgtcataatt gtaaacaccg aactctggct
     7201 cggcttcatc cagccagaac agtttcacgc ctttatcgta ataattgcgt ttggctttgt
     7261 tccagacata ttcccgtgcg ccaggatggg tagcatcaaa gaacgtggtg ttgccaagga
     7321 aatccatatt gataggcaaa ccacgctcgg tgtggaccag ccagccgttt tctttcatct
     7381 cccgataact ctcggtacgg ttatcaaccg tgggccagaa ggagaccatc agttcaatgc
     7441 ccatctcttt tagttcggca atcatggcat cgggatcggg ccagtcgcgc agatcgaaca
     7501 tccagtcacc ctgattcggc caatggaaga aatcgatgac aatcaccgaa ataggcaaat
     7561 tacgctgctt gtagccacgt gcaacttcta gtaactcttg ctgattacgg tagcgcaact
     7621 tgcactgcca gaatcccatg gcgtaatcgg gcatcattgg cggcgtaccg gtaacacgtg
     7681 cataggcggc gctgatttct gcgggagtat cccctgcggt aacccagtag tccaactgtt
     7741 cgctaacttc tgccacccat tccgtgccat tttgcgcgaa agtgacgcga cccaccgctg
     7801 ggttattcca cagaaaacca tagccgcgat tagaaagcag gaagggaacc gatgcctgag
     7861 aattacgctg cgccagctct agcatacagc cttttaaatc cagatttggc tgctggtact
     7921 gccccatgcc aaacaacttt tcgccatcgt tggcttcgaa ccgcgccttg attgtgtatt
     7981 tcccgccggg aatcggtttg aactcgcggg cctgtaaatt cagggcgctg acatactgac
     8041 cgtgggccga ctcatcttcg ccaatttcgc cgcgctgacg ccagaactcc tgcagcaggc
     8101 atttttctgc attacgccag aaagagatct gaccctgcag attaacaatg gcggtgattt
     8161 tgccgttttg tagtgttgcc tgcttttctt catatgtgat ataggcgaca gcatcacctt
     8221 tttcttctgt taatgcccag ttttcattgc gcatcatcgg cagatggcga ccgctacgca
     8281 cacgtaaact gttctcaccc catgcttcaa tccagagata acgatcgtct tgttgccaga
     8341 ccagacgatg gggatcttgc ttaataaacg acataatttc ctccaggagc ttgtgacatg
     8401 tttaagggtt atgaacttgc aaatcgtgtt ggatgcactc ttccatcgct tcatatttac
     8461 gaaagaagag cagtggaatg gcggcgagtg caaagagcac ggctggcacc cagataaacg
     8521 cccagctaat accgtccaga ccggcggcag tttgtggctg gttgggaata tagccaaagc
     8581 tgttcataat ccaggcggcg aaggcggtgc cgataccact gcccatcttg atgcagaagg
     8641 tactgccgaa ggcgattaga atgccggtag ctttaatccc tgttttccag cgaccaaaat
     8701 caaccgcgaa accaagcatg gcgaagggca tagagcaggc aatgccactg ccaatattcg
     8761 ccagcatcca ggcggcaatc atcaaaggaa tattgtctcc agccagccac atcagtcctc
     8821 cgccgaacat ggcgaccagc aggccggtga tccaaatcca ggttttggag aggtaacggc
     8881 tgaaaaacgg aattgcgatg ataaacaaaa tctgaatggt ggcgaggctg ttgatcaagg
     8941 gaactaaatc tttgcgatcc aggttgtagg ttaaatagta aacaatggtt gtgttgcgct
     9001 gctgtaaggc aatccagaag atgagatttg ccagcaccat caggatccac ggccagttgc
     9061 ctttcattgc cgaaaagctc tttttcatcg gcagacgtgg ttcctgctgt attttgtcgg
     9121 tatccagttc tcgaatattt ttgaaggcaa atagcgttag cgcacaggag attacggaaa
     9181 agacgatagc agtataaata aatcctgcct tatcattacc gtttccaaga aaagccacta
     9241 acggtagcgc cgtcgcattc attaataaca cgccaatttg cccgccggtc atccggtaag
     9301 agtttaatac caaccgttct tttggtgata acgtcatcaa aggtaatatt gcgcttagcg
     9361 gcgtgtttaa accggtaaaa agaatgctgg ctaacatgta cgatattgct gaatatatgg
     9421 ttttacctgt catactgata tctggcgacc agaatgagag tgcgctaaat acagcaaaag
     9481 gtaatggcaa ccataaaaac cacggccgac atttaccgta gcgggaatgc gttttatcaa
     9541 taataatccc ccataccggg gcatcgatac cgtctacaat acgcgccagt agcaggatga
     9601 tgccagctac ggcaaggctt atgcccgcca cgtcagtgta gaaatagaga atgtaggtgg
     9661 agccaaaaca gtagagcaga ttacctgcaa catccagcga accatagctg atccgttgta
     9721 ttaaaggcag tgaatcggat gcggatgatt tatcagtttc aagtacggaa ctcattatat
     9781 tctctccaga acaagaatgc ctggctcaga aaaacgactt ttactttcta agcaaataca
     9841 tgctaactat ccttgatgcg tatttattaa tgatgtaaag caacgttgtg gcaatgagag
     9901 taattaaagt ggggaagagc gacaattata atttttcgtt ttaaggttgc gttaattagt
     9961 tttttattca gttttcctgc tatttgttat acagttcacg ttttac
//
LOCUS       DAJOTB010000161         9971 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJOTB010000161 DAJOTB010000000
VERSION     DAJOTB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.161"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1458
                     /gene="fdrA"
                     /locus_tag="OHN28_004068"
     CDS             <1..1458
                     /gene="fdrA"
                     /locus_tag="OHN28_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2299365.1"
                     /translation="VEQAFVAMATEMNKGVLKNLGLLTPELEEAKSGDLMIVIKGTSE
                     EANERTLIAIDELFAHKEQGGQHEARYATIASAKKHAPESNLAVISVNGLFAAREARQ
                     ALQNNLNVMLFSDNVSLEDELELKHLAHEKGLLMMGPDCGTAIINGAALCFGNAVRRG
                     NIGIIGASGTGSQELSVRIHEFGGGISQLIGTGGRDMSEKIGGLMMLDAISMLEADPQ
                     TEIITLISKPPAPAVARKVLERARTCHKPVVVCFLGRESTPADEEGLQFARGTKEAAL
                     KAVLLTGVKKESLDLHPLNWQLIEEVRSRLTPQQKYIRGLFCGGTLCDEAMFTAMEKL
                     SDVYSNIHPNPAFRLTDLNRSVAHTFLDFGDDYFTNGKPHPMIDPTNRITRLLQEAQD
                     PEVGVIVMDFILGFGSHEDPVGVMLDTIVEAKEIAAADGRPLEILGYVLGTDLDTPSL
                     KKQCQMLTDAGVIWASSSTNTGLLAREFIFKGEEG"
     gene            1458..2876
                     /locus_tag="OHN28_004069"
     CDS             1458..2876
                     /locus_tag="OHN28_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2299366.1"
                     /translation="MSQTLFNQPLNVINVGIEMFSDDMKKQNVPVTQLDWTPPGQGNI
                     QVVTALDDIAASPLADKIAAANQLALERIIQSHPVLIGYDQAINVVPGMTRNTILHAG
                     PPIHWEKMCGAMKGAVTGALVFEGLAKDLDDAAELAASGEIIFSPCHEHDCVGSMAGV
                     TSASMFMHIVENKTYGNRAYTNMSEQMAKILRMGANDQSVIERLNWMRDVLGPMLRDA
                     MKLAGEIDLHLMLAQALHMGDECHNRNNAGTALLIQTLTPWIIQTGYPVEQQREVFEF
                     VLSSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRISGLPGQWFTGPA
                     QQVIGPMFAGYKLEDSGRDIGDSAITETYGVGGFAMATAPAIVSLVGGTVDEAIDFSR
                     KMREITLGENPNVTIPLLGFMGVPTAIDITRVGSTGILPVINTAIAHKDAGIGMIGAG
                     IVHPPFTCFEKAILNWRDHYCQ"
     gene            2989..4539
                     /locus_tag="OHN28_004070"
     CDS             2989..4539
                     /locus_tag="OHN28_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HCQ2299367.1"
                     /translation="MNSIKLEWKRGDWAAYFGLMTNNLTNLLTMMGLLIFVVGIPTEI
                     VYGRIAPAFGLAVLAASVCYSWFGLQMVKKTGRSDVTALPSGPSAPSIFTVTFLVLMP
                     VYQQTKDANFAIQIGLVWCFVEALILVGGSFLGETIRKMIPRTVLLSCLSGLGLLLLA
                     MNPMLQAFEAPTVSFIVLLLIFINWFGKKPIFPHIPTGLLLLIAGTSLAWISGLQSPE
                     DIKASMSSFGFNPPEIHVRSFLQGLPHALPYLASAVPLGLANYIFDLENIESAHAAGD
                     EYNTRKVMMANGFASMLGCMLGNPFPVTVYVGHAGWKAMGASICYTLASGITMFLVPL
                     FGLGAFMLAIIPMTAIVPILVFIGVVTANQVVRETPKVEVPVIFICLFPWVANWALTI
                     VNSVMGAAGTSAEKLGSDLLHSKGVYYDGLMHLGSGAPLASMLWGCIAIFAIMNKPLR
                     GAIAASFGALLSLFGVIHSPAVGFAEGSSIVFVVAYLMMLAMFALKHVIDSRETVAAS
                     EQEETKTT"
     gene            4566..5528
                     /locus_tag="OHN28_004071"
     CDS             4566..5528
                     /locus_tag="OHN28_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCQ2299368.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     PHGEKKAVTVVTQVEVDKNDPGFVHPTKPIGAFFSENQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKTLIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     SSALLAREIHADILVITTGVEKVSIHFGKPNQHALDTVDVLTMARYMQEGHFPPGSML
                     PKILASLEFLERGGKRVIITTPECLSAALRGETGTHIIHSQEET"
     gene            complement(6364..7585)
                     /locus_tag="OHN28_004072"
                     /pseudo
     CDS             complement(6364..7585)
                     /locus_tag="OHN28_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            7685..8049
                     /locus_tag="OHN28_004073"
                     /pseudo
     CDS             7685..8049
                     /locus_tag="OHN28_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(8104..8883)
                     /gene="istB"
                     /locus_tag="OHN28_004074"
     CDS             complement(8104..8883)
                     /gene="istB"
                     /locus_tag="OHN28_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCQ2299369.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDETGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(8883..9905)
                     /gene="istA"
                     /locus_tag="OHN28_004075"
     CDS             complement(8883..9905)
                     /gene="istA"
                     /locus_tag="OHN28_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCQ2299370.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
BASE COUNT         2338 a         2488 c         2658 g         2487 t
ORIGIN      
        1 gttgagcagg catttgttgc tatggcgaca gagatgaata agggggtgct gaagaatctc
       61 ggtctgctga cccctgagct ggaagaggcg aaaagtggcg atctgatgat tgtcattaaa
      121 ggtaccagcg aggaggcgaa cgagcggacg ctaatagcga ttgatgagct gtttgcgcat
      181 aaagaacagg gaggccagca cgaagcacgc tatgccacga ttgccagtgc gaagaagcat
      241 gctccagaga gtaatctggc ggtgatctct gtcaatggcc tgtttgctgc tcgtgaagct
      301 cgtcaggcac tacaaaataa cctcaatgtt atgctgtttt ctgacaacgt ctctcttgag
      361 gacgagctgg aactcaagca tctggcacat gaaaaagggt tgttgatgat ggggcctgat
      421 tgtggaaccg ccatcattaa cggcgcagcg ttgtgttttg gcaatgcagt gcgtcgcggc
      481 aacatcggaa tcattggtgc ctccggtacg ggtagccagg agctgagcgt acgcattcac
      541 gaatttggcg gcgggatttc ccagctgatt gggacaggtg gacgcgatat gagtgaaaaa
      601 attggcggcc tgatgatgct tgacgcgatt agcatgctgg aggccgatcc acaaactgag
      661 atcattacgc ttatatccaa acctcctgca ccagcggtag cccgtaaggt gctggaacgt
      721 gcgcgcacct gtcacaagcc tgtggtggtg tgtttcctcg gacgtgagtc cacccctgcg
      781 gatgaagaag gcctacagtt tgcccgcggt actaaagagg ctgccttgaa agcggtactg
      841 ttgactggtg tgaaaaaaga gagtctggat ttgcatccgc tcaactggca gctgattgaa
      901 gaggttcgct cccgtctgac tccacaacaa aagtatatcc gtggcctgtt ctgtggtggc
      961 acgttatgcg atgaagcgat gtttactgcc atggaaaaac tctcggatgt gtatagcaat
     1021 attcacccga atccggcgtt ccgccttaca gatctcaacc gtagcgtggc gcataccttc
     1081 ctcgatttcg gcgatgatta ttttaccaac ggcaaaccac atccgatgat cgatcccacc
     1141 aatcgcatta cccggctctt gcaggaagca caggatcctg aagttggcgt gatcgtgatg
     1201 gacttcattc ttggatttgg atcacatgaa gatccggttg gagtgatgct ggatacaatt
     1261 gttgaagcca aagaaattgc tgccgcagac ggtcgcccgc ttgagatcct cggctatgtg
     1321 ctgggaaccg accttgatac accgtcgctg aaaaaacagt gtcagatgtt gaccgatgcc
     1381 ggggttattt gggcaagcag tagcaccaac accggattac tggcacgtga atttatattt
     1441 aaaggggagg aaggctgatg agccagacac tgtttaatca gccgctgaac gttattaatg
     1501 tcggtatcga aatgttcagc gatgacatga aaaagcagaa tgtaccggta acccaacttg
     1561 actggacacc gccaggccaa ggaaacatac aagtggtaac ggcactggac gacattgccg
     1621 cttcaccact ggcagacaaa attgcagctg ctaatcagct ggcgctggaa cgcattatcc
     1681 agtcgcatcc tgtgctaatt ggctatgatc aggcgatcaa cgtcgtgccg ggtatgaccc
     1741 gtaacactat ccttcacgcc gggccgccaa tccactggga aaaaatgtgc ggcgcaatga
     1801 agggcgcagt aaccggggct ctggtatttg aaggactggc gaaagatctg gatgatgccg
     1861 ccgaactagc tgcttcaggt gaaatcatct tctctccgtg ccacgaacat gactgtgtcg
     1921 gctcaatggc gggtgtcacg tctgcatcga tgtttatgca catcgtcgag aacaaaacct
     1981 atggaaaccg cgcatatacc aatatgagcg agcagatggc aaagatcctg cgtatgggcg
     2041 ctaacgacca gagcgttata gagcgtctta actggatgcg agatgtactg ggacctatgt
     2101 tgcgcgatgc tatgaagctg gcgggcgaaa tcgatctgca cttgatgtta gcgcaggcgc
     2161 tgcatatggg cgatgagtgc cataaccgca acaacgccgg gacggcgctg ctgattcaga
     2221 cgctgacgcc gtggattatt cagactggct accctgttga gcagcagcgt gaggtgttcg
     2281 agtttgtcct cagtagtgat tacttctcag gaccaacatg gatggcaatg tgtaaagccg
     2341 caatggacgc tgcgcacggc attgaataca gcaccgtggt gacaacgatg gcacgcaatg
     2401 gtgttgagtt cggtcttcgt attagcggtt tgccgggcca gtggtttacc ggtccggcgc
     2461 agcaggtgat tgggccgatg tttgcgggct ataagctaga ggattccgga cgtgatatcg
     2521 gtgatagtgc aattaccgaa acctacggcg ttggcggttt cgctatggcg acggcaccag
     2581 ctattgtgtc actggtgggc ggaacagttg atgaagctat tgatttctca cgtaaaatga
     2641 gagaaatcac tctcggagaa aaccccaacg tcaccattcc cctgcttggt tttatgggcg
     2701 taccgaccgc tatcgatatt acgcgtgtag gcagtacagg cattctgccg gtaattaaca
     2761 cagccattgc gcacaaagac gcagggatcg gcatgatcgg cgcaggaatt gttcacccgc
     2821 cgttcacttg ttttgaaaag gcgatcctca actggcgtga ccactactgc caataacaat
     2881 aaccggggct aacgcagtta gccccacagt agcggaaaac gttgtttacc ttcagggctg
     2941 ggcgatctcc atgatcgcaa acgatgctta acctgttaag gagattccat gaacagcata
     3001 aaactagaat ggaaacgtgg tgactgggcg gcttactttg gactgatgac caacaatctg
     3061 actaacctgc tgacaatgat gggtctgcta atttttgtcg tcggaatccc tactgaaatt
     3121 gtctatggac gcattgcgcc tgcctttggt cttgcagtgc tggcggccag tgtctgctac
     3181 tcctggtttg gtctacagat ggtgaagaaa accggtcgta gcgatgtaac ggcgctgccg
     3241 tctggaccga gcgcaccctc aatctttacc gtgacattcc tggtgctgat gccggtttat
     3301 cagcaaacca aagatgccaa ctttgctatt cagataggat tggtgtggtg ctttgtcgaa
     3361 gcgttgatcc tggtcggtgg atccttcctg ggtgagacta tccgcaagat gatcccgcgt
     3421 acagtgctgc tttcttgtct gtccggactg gggttattac tgctggcaat gaacccgatg
     3481 ctgcaagcgt tcgaagcgcc gacagtttct ttcatcgtcc tgttattgat cttcattaac
     3541 tggttcggta agaagccgat ttttccccat attcctaccg gtttgctgtt gctgattgct
     3601 ggtacgtccc tagcgtggat atccggttta caaagcccgg aagacattaa agcgtcgatg
     3661 tcttccttcg gcttcaaccc gccggaaatt catgtaagga gcttcctgca aggtttgccg
     3721 cacgctctgc catatctggc ttctgcagtt cctttgggcc tggctaacta catttttgac
     3781 ctcgagaaca ttgaaagtgc gcacgcggct ggtgatgaat acaacacccg taaggtcatg
     3841 atggcgaacg gctttgcctc catgctcggt tgcatgctgg gtaatccgtt cccggtaacc
     3901 gtatatgttg ggcatgcagg ctggaaagct atgggcgcta gtatctgcta taccctcgca
     3961 tcaggcatca ctatgtttct ggtgccattg tttgggttgg gcgcgtttat gctcgccatt
     4021 attcccatga ctgctatagt gccaattctg gtttttatcg gtgtggtaac cgccaatcag
     4081 gtggtgcgag aaactcctaa agtcgaggtt cccgtcattt tcatctgtct gttcccatgg
     4141 gtcgccaact gggcattgac catcgttaac agcgtgatgg gcgcagccgg aacatccgct
     4201 gagaaacttg gtagcgacct gttgcacagc aaaggcgtct attacgatgg actgatgcat
     4261 ctgggaagcg gtgcaccgct ggccagcatg ctgtggggat gcattgctat ctttgccatt
     4321 atgaataagc cgctgcgcgg ggcgatagct gcatcctttg gagcactgtt atcgctgttt
     4381 ggcgtgattc actccccagc ggttggcttt gcggaaggca gctcgattgt gtttgttgtg
     4441 gcctacctga tgatgcttgc tatgtttgcc ctcaagcatg ttattgatag ccgtgaaaca
     4501 gtggctgcgt ctgagcagga agagactaaa acgacctgaa tagcctgcaa acggaagata
     4561 attctatgaa agaacttgtg gtcgttgcca ttggtggcaa cagcattatc aaagataacg
     4621 ccagccagtc gattgagcat caggcggagg cggtgaaggc cgtcgccgat acggttctgg
     4681 aaatgctggc ttccgattac gacattgtgc tgacccacgg caacgggccg caggtcggtc
     4741 tggatttacg ccgcgcggag atagcccacg agcgcgaagg gctcccctta acgccgctgg
     4801 cgaactgtgt ggcggatacg caaggcggca ttggctatct gatccagcag gcgctgaaca
     4861 accggctggc gcctcacggc gagaaaaaag ccgtcaccgt ggtgactcag gtggaagtgg
     4921 ataaaaacga tccggggttt gtccatccca ccaagcccat cggtgcattc tttagtgaaa
     4981 accagcgtga tgaactacaa aaggcaaacc ctgactggcg ttttgttgaa gatgccggac
     5041 ggggctatcg ccgcgtggtc gcctcgccgg aaccgaaacg tattgtcgaa gcacctgcca
     5101 ttaagactct gatccagcaa ggctttgtgg tgattggcgc gggcggcggt ggcattccgg
     5161 tggtgcgtac tgacgcaggg gattaccaaa gcgtggacgc ggttatcgac aaagatctct
     5221 ctagcgcgct actggctcgc gaaattcacg ccgacattct ggtgatcacc accggcgtgg
     5281 aaaaagtgag tattcatttt gggaaaccca accagcatgc actggataca gtggatgtcc
     5341 taacgatggc ccgttacatg caggagggtc acttcccgcc tgggagtatg ttaccgaaga
     5401 ttcttgccag tctggagttc ctggaacgtg gaggtaaaag agtgattatc accacgcctg
     5461 aatgcctgtc cgcggcactg cgtggcgaaa caggcaccca tattatccac tcccaagagg
     5521 agacgtaaat taaggaaagc gaccgccgcc gtgaattttt aatgttcatt gcagattatc
     5581 gtgggctgga aatgataacg gttggggagt tattgtttag ttgctaaaac agaacgataa
     5641 ccaaccccaa ccgttctcaa tttcgaatac tatcttttcc atttatctct ggataggctt
     5701 tgacgtcgtc cctactgagt aaaatccccc tcatcatcca cctaaaaccg attgaaaacg
     5761 gtcattactc ttgtggcaaa caggctaaag ccgttcacga tgcttcactt cgcaccttat
     5821 cgtaatgaac aatactggta tttccagttc agttacgcct acccgtctac cgcattcttt
     5881 gccctaacta cggcatagtt cgggattata tcagctggct aaagccttat gctcatcaga
     5941 ccacatgact cattgagcat gttgaggcgt tactcaaaat cttgccgatt aaacatattt
     6001 cgtaatttct taccctgcac tagcatatca tcaatactat cgacaaacgt cagttgcaaa
     6061 tagaggtcac agggtctgat tgatgtactg ccaaggccgg gtcatgaaag agtttgccct
     6121 gttcaaagct catcgttacg caccgtcgta gccgatgcgg atactcatca atcctctgga
     6181 taggtgaggt gcgtagtaga gaagctcggt cgtttttcgt taatctgaat cagtactgtc
     6241 aacatattca agtcgttaat atggatatga atacctcctt tgacctagag gtacagatgc
     6301 actacccccc aagctgtcaa cgacggatga aaagtggctc ttccgcaacc tggatggaga
     6361 ttttcatgcc agtggactca tgaccctctg cctgagtagt tcaaatccgg ctcgaccata
     6421 catctggcct ttcagcattt ttagccgatt tacatgactt tcgacacccc ccattattcc
     6481 agcgactgct gattgcctca catatagccg cagcatcagc tgccatcctt gcagccacgc
     6541 gccgaagtta ccactttctc gttgctcttt taaccactcc ctccatgggt ccagaagtcc
     6601 tcgctctgga ggccgggttg acatttcagg gaatgttcct gaacacaccc agagactgac
     6661 tgtcacacgt gataagcctg taatacggga taagcctgta atacgggata tttcccctga
     6721 acccacatcc cttgttatgt agtgccataa cctctgtcca acgctgatgc cgtttttggc
     6781 gggtttgctg tttaaggcgt tccggacgac ggagcgatgc tacaggcaca gacagtactg
     6841 gcttaggtga tttctttggt gacaacccac tggcaacaag acgtatcaga ggcatctgtc
     6901 tgtacatcat tcgttcaggc gcatcgccaa tattttttag caggtgccat cgatcagcta
     6961 tctgtctggc ctgaggtgca ccttcacgtg ctgctgtcgc atagactccg ccacggtcac
     7021 gtgagacaac ctgtatttcc ggatattttc tgaaccaggt cgccagcgta ctctgatcgc
     7081 aaccgggaag caggacgagg ggacggtgag tatcaagatt gacgattaac gtgccgtaat
     7141 ggtggccccg atgccacgcc cactcatcaa taccgacatg aggcgcgccc gactgtttcg
     7201 tctccggggt attaataacc ctgcgaagaa gagtatcagc actgcaacgg agccccactg
     7261 ccgttgctgc ctgtttcccg gcctcacctc cggctattaa tcccagttga tgttgtaaat
     7321 tttgtaacac ctgtgaagaa tgctgggagt gaaccggcga agggggcaag cgacccggca
     7381 aaaattttac gtgaacaagc cgggttacgg cagtaccagt ggcggacgga aaatacaagc
     7441 catagcgtct gccctgagca gggtagatgc tgtatccggc gccgacgata actatgaaca
     7501 gagtcactac gcttaaagca ttcaggactt gtgctgtttt gcgttttccg tggagatgga
     7561 gggttatacc gtcagagcta ataatttgtc gactgcatct ccatccgcaa ggtaactgta
     7621 gaagtgtctg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     7681 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     7741 taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg
     7801 gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat
     7861 ggccatgaat ggcgttggat gccgggcaac tgcccgcatt atgggcgttg gcctcaacac
     7921 gattttacgt cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc
     7981 agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatgt
     8041 caacgacgga tgaaaagtga tccacttata tctccaccaa cggcccaata ttgatccacc
     8101 gttttactca ggattagctt ctgctataac cccggctttt cgtttctgtc tgagtcgata
     8161 gctttctcct ttgatttgaa cgacatgtga gtggtgtaag atacggtcca gcatcgctga
     8221 ggtcagtgct gcatcaccgg cgaacgtttg atcccactgc ccgaacggca gattggatgt
     8281 caggatcatt gcgctctttt cgtaacgttt agcaatgacc tggaagaaca gttttgcttc
     8341 ttcctgactg aacggcagat agcctgtttc atcaatgatg agcaggcggg gggccattac
     8401 tccacgctga agcgtcgttt tataacggcc ctgacgttgt gccgtagata actgaagtaa
     8461 cagatctgct gctgttgtga agcgaacttt gatacctgca cggacggctt catagcccat
     8521 cgctattgcc agatgggttt tccccacacc tgatggcccc agtaatacga tattttcatt
     8581 acgttctatg aagctgagtg agcgtaacga ctggagttgc ttctgcggtg ctccggtggc
     8641 gaatgtgaag tcatactctt cgaacgtttt caccgccggg aaggctgcca ttcgggtata
     8701 catcgcctgt ttacgttgat gacgtgccag tttttcttca tgaagcagat gctccaggaa
     8761 gtccatataa ctccattcct ggtctactgc ctgttgtgac agcgcaggcg cagcgcttat
     8821 aaggctttcc agttgcaact gcccggcgag cgccatcagt cgttgatgtt gcagttccat
     8881 catcacgcca ctcctctgca gaatgagtcg tagatggaga gtggatgatg cagggggtgt
     8941 ttgtcgaagt tcaccagatt ttcaccagga tgcacgtcat actctttttt ctccggaggc
     9001 agtgccagca tggactgctg ctcttcgagc cagcgatcgc agggacgggc ctggattgtt
     9061 tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg gttggctgtt
     9121 tcaacatcga cagtgatccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg
     9181 ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct gaaggggcga
     9241 cacagtcggg gagagaagcc catctccttg ccgaactgcc acagcgaagg atggaaccgg
     9301 tgctgaccgg tctgatatgc gtcacgttgc agaaccacag ttttcatatt gtcatacaac
     9361 acttcgcgcg gcacaccacc aaagaagcgg aacgcattac gatggcaggt ctccagcgtg
     9421 tcataacgca tattgtcagt gaattcgatg tacaacattc ggctgtatcc gagaacagca
     9481 acgaacacgt gaagcggtga gcggccatta cgcatagtgc cccagtcaac ctgcatctgt
     9541 cgtccgggtt cagtttcgaa ccgaacggca ggctcctgct cctgaggaac cgagagagaa
     9601 cgaatgaatc ccctgagaat ggtcattccg ccacgatatc cctggtctct gatctcgcga
     9661 gcgattaccg ttgccgggat tttgtaagga tgagcatcgg cgatgcgttg acgaatataa
     9721 tcccggtatt catccaggag tgaagcaaca gcaggtcgcg gcgtatattt tggcggctca
     9781 gattttgcct gcaaataacg tttaaccgta ttgcgggaga tccccagttc tctggcaatc
     9841 gcccggctac tcattccctg cttgtgcagg attttaattt ccataactgt ctcaaaagtg
     9901 accataagct ctcctgaatc aggagagcag attaccccct ggatctgatt tcaggcgttg
     9961 ggtgtggatc a
//
LOCUS       DAJOTB010000162         9951 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJOTB010000162 DAJOTB010000000
VERSION     DAJOTB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.162"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            150..200
                     /gene="hisL"
                     /locus_tag="OHN28_004076"
     CDS             150..200
                     /gene="hisL"
                     /locus_tag="OHN28_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCQ2299371.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    176..299
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            346..1245
                     /gene="hisG"
                     /locus_tag="OHN28_004077"
     CDS             346..1245
                     /gene="hisG"
                     /locus_tag="OHN28_004077"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCQ2299372.1"
                     /translation="MTDNSRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1251..2555
                     /gene="hisD"
                     /locus_tag="OHN28_004078"
     CDS             1251..2555
                     /gene="hisD"
                     /locus_tag="OHN28_004078"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCQ2299373.1"
                     /translation="MSFNTIIDWSSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2552..3622
                     /gene="hisC"
                     /locus_tag="OHN28_004079"
     CDS             2552..3622
                     /gene="hisC"
                     /locus_tag="OHN28_004079"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCQ2299374.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVSTLDNWQLDLQSISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALQEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3622..4689
                     /gene="hisB"
                     /locus_tag="OHN28_004080"
     CDS             3622..4689
                     /gene="hisB"
                     /locus_tag="OHN28_004080"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCQ2299375.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLRLQ
                     KAGYKLVMITNQDGLGTQSFPQADFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4689..5279
                     /gene="hisH"
                     /locus_tag="OHN28_004081"
     CDS             4689..5279
                     /gene="hisH"
                     /locus_tag="OHN28_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCQ2299376.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIEED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5279..6016
                     /gene="hisA"
                     /locus_tag="OHN28_004082"
     CDS             5279..6016
                     /gene="hisA"
                     /locus_tag="OHN28_004082"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCQ2299377.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5998..6774
                     /gene="hisF"
                     /locus_tag="OHN28_004083"
     CDS             5998..6774
                     /gene="hisF"
                     /locus_tag="OHN28_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCQ2299378.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6768..7379
                     /locus_tag="OHN28_004084"
     CDS             6768..7379
                     /locus_tag="OHN28_004084"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCQ2299379.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNILNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLATVIENLRKRHQ"
     gene            complement(7564..8541)
                     /gene="wzzB"
                     /locus_tag="OHN28_004085"
     CDS             complement(7564..8541)
                     /gene="wzzB"
                     /locus_tag="OHN28_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCQ2299380.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8687..9853)
                     /gene="ugd"
                     /locus_tag="OHN28_004086"
     CDS             complement(8687..9853)
                     /gene="ugd"
                     /locus_tag="OHN28_004086"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCQ2299381.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGTIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         2436 a         2576 c         2621 g         2318 t
ORIGIN      
        1 agaggacagt cctcttagcc cccttctttc cccgctcatt cattaaacaa atccatcgcc
       61 ataaaatata taaaaaagcc cttgctttct aacgtgaaag tggtttaggt taaaagacat
      121 cagttgaata aacattcaca gagactttta tgacacgcgt tcaatttaaa caccaccatc
      181 atcaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt cagatcttcc
      241 agtggtgcat gagcgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt
      301 gcgcggttga taacggttca gacaggttta aagaggaata agaaaatgac agacaactct
      361 cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg cgaattgctg
      421 gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat ggcagaaaac
      481 atgccgattg atattctgcg cgtacgtgat gacgacattc ccggtctggt aatggacggc
      541 gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct taaccgccgc
      601 gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg cggctgccgc
      661 ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt aaacggtaaa
      721 cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa aggtatctct
      781 tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgcgccgg actggcggat
      841 gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct gcgcgaagtc
      901 gaagttatct accgctcgaa agcctgcctc atccagcgcg atggcgaaat ggaagaatcc
      961 aaacagcaac tgatcgacaa actgctgacc cgtattcagg gtgtgatcca ggcgcgcgaa
     1021 tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat cgccctgctg
     1081 ccaggtgccg aacgcccaac tattctgccg ctggcgggtg atcaacagcg cgtagcgatg
     1141 cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa agcgctgggt
     1201 gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc atgagcttta
     1261 acacaatcat tgactggagt agctgtactg cggagcaaca acgccagctg ttaatgcgcc
     1321 cggcgatctc agcttctgaa agcattaccc gcaccgttaa cgatattctc gataacgtga
     1381 aaacgcgtgg cgatgaagcc ctgcgtgaat acagcgcgaa gtttgataaa accacggtta
     1441 ccgcgctgaa ggtgtctgct gaggaaatcg ccgccgccag cgaacgcctg agcgacgagt
     1501 taaaacaggc gatggcggtg gcagtaaaga atattgaaac cttccacact gcgcaaaaac
     1561 tgccgccggt agatgtagaa acgcagccag gcgtacgttg ccagcaggtc acgcgcccgg
     1621 tggcttcagt gggtttgtat attcctggcg gctccgcccc gctcttctca acggtattaa
     1681 tgctggcaac tccggcgcgt attgcaggtt gcaataaagt ggtgctgtgc tcaccgccgc
     1741 cgattgccga tgagatcctt tacgcggcgc agctgtgcgg cgtgcaggac gtgtttaacg
     1801 tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg ccgaaagtgg
     1861 acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag gtaagccagc
     1921 gtctggacgg tgcggcgatc gatatgcccg caggtccgtc ggaagtgctg gtgattgctg
     1981 acagcggcgc tacgccggat ttcgtggctt ctgatttgct ttctcaggct gaacacggcc
     2041 cggactcaca ggtgatttta ctgacccccg acgccgatat ggcgcgtcgc gttgccgagg
     2101 ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cgcccgccag gcactgagcg
     2161 ccagccgcct gatcgtgact aaagatttag cgcagtgcgt agagatttct aatcagtacg
     2221 gcccggagca cctgatcatt cagacccgca acgcccgcga gctggtcgat ggcatcacca
     2281 gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac tatgcctccg
     2341 gtaccaacca cgtactaccg acttacggtt ataccgcgac gtgttccagc ctcggcctgg
     2401 ctgatttcca gaagcgtatg accgtgcagg agctgtcgaa agaaggtttc tccgcgctgg
     2461 cgtcgaccat tgaaacactg gccgccgccg agcgcctgac tgcccacaaa aatgccgtta
     2521 ctttgcgtgt taacgccctt aaggagcaag catgaacacc gtgactatta ccgatttagc
     2581 gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg cgtcgtctgg gcggtaacgg
     2641 cgacgtctgg ctgaacgcca acgaataccc cacagccgtg gagtttcagc tcactcaaca
     2701 aacgctcaac cgctatccgg aatgtcagcc aaaagcggtg attgaaaatt acgcgcaata
     2761 tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag gtattgaact
     2821 gctgattcgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc cgccaacgta
     2881 cggcatgtac agcgtcagcg ctgaaaccat tggcgtcgag tgccgcacag tgtcgacgct
     2941 ggacaactgg caactggact tacagagcat ttccgacaag ctggatggcg taaaagtggt
     3001 ctatgtttgc agccccaaca accccaccgg gcaactgatc aacccgcagg attttcgcac
     3061 cctgctggag ttaacccgcg gtaaggcgat tgtggttgcc gatgaagcct atatcgagtt
     3121 ttgcccgcag gcatcgctgg ctggctggct ggcggaatat ccgcacctgg ctattttgcg
     3181 cacactgtcg aaagcttttg ctctggcggg gcttcgttgc ggatttacgc tggcaaacga
     3241 agaagtcatc aacctgctga tgaaagtgat cgccccctac ccgctctcga cgccggttgc
     3301 cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgcgcg aacgggtggc
     3361 gcaaattatt gcagaacgcg aatacctgat tgccgcactg caagagatcc cctgcgtgga
     3421 gcaggttttc gactccgaaa ccaactacat tctggcgcgc tttaaagcct ccagcgcagt
     3481 gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac aaccctcttt
     3541 aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg tcattgacgc
     3601 cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg cgatggaacc
     3661 ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact cgcctttgaa
     3721 cctggcgtga tcccggagct gctgaggctg caaaaagcgg gctacaagct ggtgatgatc
     3781 actaatcagg atggtcttgg aacacaaagt ttcccgcagg cggatttcga aggcccgcac
     3841 aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt gctgatttgt
     3901 ccacacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact ggtggagcgt
     3961 tatctggctg aacaagcgat ggatcgcgcc aacagttatg tgattggcga tcgcgcgacc
     4021 gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg cgaagccctg
     4081 aactggccga tgattggtga gcaactcact aaacgagacc gttacgccca tgtagtgcgc
     4141 aacaccaaag agacgcaaat tgacgtccag gtgtggctgg atcgcgaggg tggcagcaag
     4201 attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac ccacggcggt
     4261 ttccgtatgg aaatcaacgt caaaggcgac ctctatatcg acgatcacca caccgtcgaa
     4321 gataccggcc tggcgctggg cgaagcgtta aaaattgccc ttggcgacaa acgcggtatt
     4381 tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc gctggatatc
     4441 tctggtcgtc cgcacctgga atataaagcc gagtttacct accaacgcgt gggcgatctc
     4501 agcaccgaga tgatcgagca cttcttccgt tcgctttcat acaccatggg cgtgacgcta
     4561 cacctgaaaa ccaaaggtaa aaacgatcac caccgtgtag agagcctgtt caaagcgttt
     4621 ggccgcaccc tgcgccaggc catccgcgtg gaaggcgaca ccctgccctc gtcgaaagga
     4681 gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac tcggtgaagt
     4741 ctgccattgc gcgtcacggt tatgaaccca aagtcagccg tgacccggac gtcgtgttgc
     4801 tggccgataa actgttttta cccggcgttg gcaccgcgca agcggcgatg gaccaggtgc
     4861 gcgagcgcga gttgtttgat ctcatcaaag cctgtaccca accggtgctg ggcatctgct
     4921 tagggatgca actgctgggg cggcgcagcg aagagagcaa cggcgtcgat ttgctgggca
     4981 tcatcgaaga agacgtaccg aaaatgaccg actttggtct gccactgcca catatgggct
     5041 ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa gacggcgcgt
     5101 acttttactt tgttcacagc tacgcaatgc cggtcaatcc gtggaccatc gcccagtgta
     5161 attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc gtgcagttcc
     5221 acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg gagatgtgat
     5281 gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc agggcgatta
     5341 cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt acgctgcgca
     5401 aggtgccgaa gtgttgcatc tggtggatct gaccggggca aaagatccgg ctaagcgtca
     5461 aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg ttggcggcgg
     5521 tgtgcgtagt gaagaagacg tagcgacgtt actggaagcg ggcgttgcac gcgtggtggt
     5581 tggctccacc gcggtgaaat caccagaaat ggtgaaaggc tggtttgaac gtttcggtgc
     5641 tgatgcctta gtgctggcgc tggatgtccg tattgacgag caaggcaaca agcaggtggc
     5701 ggtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg aaacctatct
     5761 gcccgtcggc ctgaaacatg tgctgtgtac cgatatctcg cgcgacggca cgctggcggg
     5821 atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg cgtttcagtc
     5881 ctccggcggc attggcgata ttaacgatgt ggcggccctg cgtggtactg gtgtgcgcgg
     5941 cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg ccatcgcatg
     6001 ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt gaaaggcgta
     6061 cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg ctacgccgaa
     6121 gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg ccgtgtggta
     6181 gataaaagct gggtatctcg cgtggcggaa gtaatcgaca ttccgttttg tgtggcgggt
     6241 gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga taaaatttcc
     6301 atcaactctc ctgcgctggc ggacccgacg ttaattactc gcctggccga tcgctttggc
     6361 gtgcagtgta ttgtggtcgg tattgatacc tggtacgacg ccgaaaccgg taaatatcat
     6421 gtgaatcaat ataccggcga tgaaagccgc acccgcgtta ctcagtggga aacgctcgac
     6481 tgggtacagg aagtgcaaaa acgcggtgcc ggagaaatcg tcctcaacat gatgaatcag
     6541 gacggcgtgc gtaacggtta cgacctcgag cagctgaaaa aagtgcgtga agtttgccac
     6601 gtcccgctga ttgcctccgg tggcgcgggc accatggaac actttctcga agccttccgc
     6661 gatgccgacg ttgacggcgc gctggcagct tccgtattcc acaaacaaat aatcaatatt
     6721 ggtgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg ttaacagaac
     6781 aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg attgtgcaac
     6841 acgcggtatc cggcgaagtg ctaatgctgg gctatatgaa cccggaggcc ttagacaaaa
     6901 ccctcgaaag cggcaaagtc accttcttct cgcgcactaa acagcgactg tggaccaaag
     6961 gcgaaacgtc gggcaatatt ctcaatgtgg tgagtattgc cccggactgc gacaacgaca
     7021 cgttactggt gctggcaaat cccatcggcc cgacctgcca caaaggcacc agcagctgct
     7081 tcggcgacac cgctcaccag tggctgttcc tttatcaact ggaacaactg ctcgccgaac
     7141 gtaaatctgc cgacccggaa acctcctaca ccgccaaact gtatgccagc ggcactaaac
     7201 gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca acggtacatg
     7261 accgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg gtgttgttgc
     7321 aggatcagga tctggattta gcgacggtaa ttgagaacct gcgtaaacgg catcagtgag
     7381 ttgcgtgctg accggatgcg atgttgttgt cgcatccgga gccattgccc gatgcgcaag
     7441 cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg ccagcatcgc
     7501 atccggcaaa aaaacgggca tggtgtcacc accctgcccg tttctcttaa atgcacaata
     7561 atattatttc gcgttgtaat tacgcagagc gttacgccca agcacaatcc ccgcgccaac
     7621 catgccgccc agcagcaccg ccagaatcaa ggtaattgcc tttttcgggc tatcgcgacg
     7681 aataggtaac gtcggtttca ttacatagcg gtaagcatga atatcaagat catcaacctt
     7741 taagttttca atatccagca ggttttgacg agtctgatag tagtttgatg agaacaccaa
     7801 cggacgagtc gcctcgtgct taatcatcga ctccagcgct tcgctcccca aaaggaacat
     7861 agtatcctgg gttacgtcct gtgtctgttg aatctgcggc tttgtcacct gcgcctgatt
     7921 cgcatactgc aacgcttcct gaatctgacg gatacgcaga tctttctgtt cttgagcgac
     7981 cacttcctgc gtcttcagag aatcttgcaa gtttttcatt cgcagcgcga tgttgtcttt
     8041 caggtcttgt tccagttctt catttacctg gtcgtcaact tgctggatat acttcgccag
     8101 ctgtttctgc gcaccttcag gtgtttcacc aacataagac accgccaatg gtaacgattg
     8161 gtttttaacg gtaggctcaa tggtcagctt ttctgcttct tcctggttat ccagcgtttc
     8221 cgctaatgct gaaaatgcag tgctgtaacg tccgataagt gaagcctgaa tgtcggagac
     8281 tttcggtact gccggaccgt aaatcacatt catcgcgttg gtataaccag cgatttgccc
     8341 cacatcaggt tgagtaacga tagctgttga agtccacttt tctttggcaa cggccagata
     8401 ccctatagcc agggcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca
     8461 caactgcact agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac
     8521 attattattt tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt
     8581 ttagcgataa tcctcgtgaa tgctatagga taagtgataa aaataatgaa ttgttgagga
     8641 agtaacaaaa gacaatcagg gcgtaaatag ccctgataac aagatgttaa tcgctaccaa
     8701 agagatcgcg ggtgtatacc ttatctgcca catccttaag ctcttctgcc atacgattgg
     8761 agataatgac gtcagcttgt tgtttgaaag tggcgagatc tcgttccagg cgagagttga
     8821 agaatgagtc ttctttcatc accggttcgt aaatgatcac ttcaacgcct ttcgccttga
     8881 tacgtttcat aatcccctga atggaagacg cgcggaagtt gtctgaaccg ctcttcataa
     8941 tcagacgata aatacccacc acttgcggct tgcgtgacaa aatggcatcg gcaataaaat
     9001 ctttacgcgt gcggttagca tcgacaattg ccgaaatcag gttattcggc acagactgat
     9061 aattcgccaa taactgtttg gtatctttcg gcaaacaata accaccataa ccaaacgacg
     9121 gattgttgta atggttgccg atacgcgggt cgagacaaac accttcgata atctgacgcg
     9181 tattcagacc caaactttct gcatagctat ccagttcgtt aaagtacgcc acgcgcatcg
     9241 ccagataggt attagcgaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg
     9301 tcgggatatt ttgcttaatc gtgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt
     9361 ctgaacgctc accgatgaca atacgtgacg gatgaaggtt atcgtaaagg gctttaccct
     9421 cacggagaaa ttccggggag aatataatat tttcagtacg atatttttta tgcatcgctg
     9481 cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataaggg tttatctcaa
     9541 ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat
     9601 agtcggttgg agtggcgatg atgacataat cagcatcccg gtaggcttca tttttatcta
     9661 atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa
     9721 taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa
     9781 cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa
     9841 tggtaatttt catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc
     9901 atcctgacaa gagttaaaag ttttaacgct acgtcgatag ctggtcaacg c
//
LOCUS       DAJOTB010000163         9927 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJOTB010000163 DAJOTB010000000
VERSION     DAJOTB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.163"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            8..2152
                     /gene="iroN"
                     /locus_tag="OHN28_004087"
     CDS             8..2152
                     /gene="iroN"
                     /locus_tag="OHN28_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HCQ2299382.1"
                     /translation="MLLVGLNSQVSVAKSSDDDNDETLVVEATAEQVLKQQPGVSVIT
                     SEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGPENTLILIDGVP
                     VTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGAAGGVVNIITKR
                     PTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGNLNKTDADSWDI
                     NSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIYAGDTQNSSSSA
                     VTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRMNEGLSGGGEGR
                     ILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDPSSTSLTVNDSD
                     ISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSDSGGNFSPSLNL
                     SQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGGCYLIGNKDLDP
                     EISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAYILKWQNGGKAL
                     VDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTINNSLNWTITQAF
                     SASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDINKNLRLNVGVS
                     NILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2197..3153)
                     /gene="iroE"
                     /locus_tag="OHN28_004088"
     CDS             complement(2197..3153)
                     /gene="iroE"
                     /locus_tag="OHN28_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HCQ2299383.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3238..4467)
                     /gene="iroD"
                     /locus_tag="OHN28_004089"
     CDS             complement(3238..4467)
                     /gene="iroD"
                     /locus_tag="OHN28_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HCQ2299384.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4571..8230)
                     /gene="iroC"
                     /locus_tag="OHN28_004090"
     CDS             complement(4571..8230)
                     /gene="iroC"
                     /locus_tag="OHN28_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HCQ2299385.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            complement(8370..9485)
                     /gene="iroB"
                     /locus_tag="OHN28_004091"
     CDS             complement(8370..9485)
                     /gene="iroB"
                     /locus_tag="OHN28_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HCQ2299386.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
BASE COUNT         2328 a         2722 c         2685 g         2192 t
ORIGIN      
        1 gctaactgtg ctcctggttg ggttgaatag ccaggtatca gtagccaaat cctccgacga
       61 tgataatgac gagactctgg tggtggaagc caccgctgag caggtattaa aacagcagcc
      121 gggcgtgtcg gttattacca gcgaggatat taaaaagacc cctccggtaa acgacctttc
      181 agatattatt cgtaaaatgc ctggcgtcaa tcttaccggc aatagcgcct cgggcacacg
      241 cggtaacaac cgccagatcg atattcgtgg tatggggccg gaaaacacct taattttaat
      301 tgatggtgta ccggtgacgt cacgtaactc cgtgcgttat agctggcgtg gagagcgtga
      361 tacccgcggt gacaccaact gggtgccacc ggaacaggtt gagcgtattg aagtgatccg
      421 cggccctgcg gcggcgcgct acggttcggg ggcagccggg ggggtggtga acatcattac
      481 caaacgtccc accaacgact ggcacggttc gctgtcgtta tacaccaatc agccggaaag
      541 tagcgatgag ggcgctacgc gtcgcgccaa tttcagcctt agtgggcctc tggctggtaa
      601 tgctcttacc acgcgtttgt atggtaacct gaataaaacg gatgctgaca gttgggatat
      661 taattctccg gtcggtacga aaaacgcagc cgggcatgaa ggggtacgta acaaagatat
      721 taacggcgtt gtctcgtgga aattaaatcc gcagcagatt ctcgatttcg aagccggata
      781 tagccgccag gggaatatct atgcgggcga tacgcaaaac agttcttcca gtgcagttac
      841 cgaaagcctg gcaaaatccg gcaaagagac gaaccgcctg taccgacaga attatggcat
      901 tacgcataat ggtatctggg actggggaca aagtcgcttt ggtgtttatt acgagaaaac
      961 caataatacc cgcatgaatg aaggattatc cggcggtggt gaagggcgta ttttagcggg
     1021 tgaaaagttt acgaccaatc gcctgagttc ctggcgaacc agcggcgagc ttaatattcc
     1081 tttgaatgtg atggttgatc aaacgctgac cgttggtgca gagtggaacc gcgataagct
     1141 cgatgatcct tcctctacca gcctgacggt gaatgacagt gatatcagcg gtatttctgg
     1201 ctctgctgcg gatcgcagca gtaaaaatca ttctcaaatc agtgcgctgt atattgaaga
     1261 taacattgag ccggttcctg gcacgaatat cattcccggc ctgcgctttg attatctcag
     1321 cgactccggc gggaacttca gccccagtct gaatctttcg caggaattgg gcgattattt
     1381 caaagtcaaa gcaggggttg cccgaacctt taaagctcca aacctgtatc aatccagtga
     1441 aggctatctg ctctactcga aaggcaatgg ctgtccaaaa gatattacat caggcgggtg
     1501 ctacctgatc ggtaataaag atctcgatcc ggaaatcagc gtcaataaag aaattgggct
     1561 ggagttcacc tgggaagatt accacgcaag tgtgacctac ttccgcaatg attaccagaa
     1621 taagatcgtg gccggggata acgttatcgg gcaaaccgct tcaggcgcat atatcctcaa
     1681 gtggcagaat ggcgggaaag ctctggtgga cggtatcgaa gccagtatgt ctttcccact
     1741 ggtgaaagat cgtctgaact ggaataccaa tgccacatgg atgatcactt cggagcaaaa
     1801 agacaccggt aatcctctgt cggtcatccc gaaatatact atcaataact cgcttaactg
     1861 gaccatcacc caggcgtttt ctgccagcgt caactggacg ttatatggca gacaaaaacc
     1921 gcgtactcat gcggaaaccc gcagtgaaga tactggcggt ctgtcaggta aagagctggg
     1981 cgcttattca ctggtgggga cgaacttcaa ttacgatatt aataaaaatc tgcgtcttaa
     2041 tgtcggcgtc agtaatatcc tcaataaaca gatcttccga tcttctgaag gggcgaatac
     2101 ctataacgag ccaggccggg cttactatgc cggagttacc gcatcattct gatcgtagtg
     2161 tttcaggcgt actgcccgcg taaaagcggg cagtgtttag tggcttaact catgacaacc
     2221 tgctgtgtaa tttgcgtttt caccactgat atctaacagt gcctggcgaa aggaggcatt
     2281 gaacatcggc ccgtgtccta ggttggggaa atcccaaaat acggcattga cgcctttatc
     2341 tttcagtata gtgagggtgg tatgaatttt cgacagcacc ccgacagcat gcgtttcccg
     2401 gttatcaccc tgtgtcgccg agccttccat tatcgccagg tgtttggcgc agaattgcag
     2461 aggctcaacc gccgtaacgc ggcttagcaa agcatcataa cctctgccca acgacgggct
     2521 ggcgctgtag tacgaccgga agtaagagga ggacagccag gaatccagca cgaagaggcc
     2581 gccgtaggag tgcccccata agccgcggcg ttgccgatcg atattcagtc cctgttccac
     2641 ttttggggca atacgcgttt ccagtaactg gcggaagttg ttgctgccac cactcttacg
     2701 gctaaaacgc cctgagtgga gatctgtttt tctgctttct gctgctggcg tatagtcgta
     2761 agccctgctg ttgagatcga aagggaggtt ggtctgatac ccgacagcca cgatcactgg
     2821 cggtgttttt tctgacaatt gtttgagcag ttcgtcatcc aggcgatcca taactgcgtt
     2881 accgtcaagc atatataaaa tcgggtaacc cgatgccggt gcggttgtat tcggcacggc
     2941 cgtccatacc cgataatggc gtgtgccatc gacagagtcg aaagaggtga cgctgaaatg
     3001 gtaaaacacg gagcctttat cggctatatt cggccccagt ggccgcatat ccggcttcgc
     3061 ataaacttgc gcactacaga taagggtgag gcaagaaaga tgaaagaaaa tcgctttatg
     3121 cgggcgtgtt gagcgatact cgcgggcata catctgatga ttattccttt tccgcaggtg
     3181 actatttgat ttattcagga agtctgtacc gtttcctgaa agtgtttgtg ggtcaactca
     3241 accctgtagt aaaccaatcc cgtcaatcag tgcaccgcgc caccatgcgt aatcgtgacc
     3301 accggtgtag attgcgcaat ggctttcgac gccagcggta atcagccgct ggtgaagctg
     3361 ctgaacgtga ggcactgtcg aaccttccag cgatcccacg cacaggctga tacgtacgcc
     3421 ctgcggtggg gcagaaagca gatgctcact cacccatgag gtatcggttt cgctgaacaa
     3481 gcctggtcga ctggttcttt ctggcgtcca ccacattgaa ggagagtggc tgagcaccag
     3541 accgaacgtt tccggtgcgt aacgagcccc cattagcgca ctgatcccgc cgaggctctg
     3601 cccggccagc actgtgcgcg aacggtctgc ccactgacgc tgcggttgtt cagcgcgaat
     3661 catcggcagc agatgtccgg cgatatcctt tatcagtttg ctgcgcccgc cgagtatctc
     3721 agtgcgttca tgttcattaa tgttgtcaat gcccagtaca gccacgggca cgatgcgccc
     3781 attatttatg gcggcgtcaa ttgccgcgca tacgccaagg tgatcaaacc aggtttcacc
     3841 atcgggcagc acgaccagcc cgaggggctg agaaatatca acatccggaa tatagagccg
     3901 aacctggcga gatttcccgg cgataatgcg catggaggta agcagttgtc cggtgtggaa
     3961 gccgccgttc cattccgaca gggcgggagc tttatcaagt gtcagcactg attcctttgc
     4021 gtttccccga acgttgatct ccggcatttt gtttagcgga tcggcctttc cggcaagggt
     4081 ggcaaaacgg cctccggaca gtgcaatcgt ctcagccgta gtgccggggg ggatttccag
     4141 cagcgaatag gagccgcagt aacttgcggg taaacgcagt gtcagtgtcc agatatccgt
     4201 ttcgggaagg gcgctcatca ttcctttttc tacgtgctct ttatccgtca cccggttcag
     4261 acgcagataa acgccctgca gaggatgtcg ggcgcgccac agaaaggtca cttctcgctc
     4321 atcttccgct ccttcgactg gcgtcacaag aggaacattc agcgactcag cttcgcgcca
     4381 gaagtcagta aggttagcaa tgtggcccgc tgccagttgg ttgcgcaggc tggcgatagc
     4441 agagggatgt tgttgcatgt tcagcattca tactctttaa aaattaagga tgtcagtata
     4501 aataattaaa gcggctattc acggtaccgc cgcttctgac tgtgttttta atacattacg
     4561 tgtccagtaa ctatcccggg gtttcctcct tcatgttgtg ctggcgatgg agcgcagtac
     4621 tgctgacgct gtcatgccat aagcgggtat agaggccgcc cgcagataac agctgttcgt
     4681 gggttccgta ttccgcaagt aaccccttat caataacggc aatcagatcg cagcgttgcg
     4741 cggtggtcag acgatgtgca acaatcagcg ccgagtgctt cccggcatgc acgacatctg
     4801 ttaacgatga catcagtcgt tcttcggatg tgcgatccag acatgacgtg gcttcgtcga
     4861 gcagcaggat gtgggcattc gccagttgcg cgcgggccag cgcaatcaac tggcgctgac
     4921 ctgcgggcag atcggctccg ccgttattca ccggtgtccg gaatccctgc ggcagattgc
     4981 acaccatctc atacagccct gcccgctgtg aggcaatttc aacgtcttca ttggtggcgg
     5041 atggccgtga ataacgaatg ttttctgcaa tatcactact aaacagtgct acatcctgat
     5101 cgacaagccc aatctggcga cgatactcag tgagcgaggc atcatcgagc atttgcacac
     5161 cgattctgat gttgccgtgc gtggggaaat acaacccggc aatcagctta atcagcgtcg
     5221 acttacccgc accgctgcga ccgacgaccg cgacaaccat tccctctggg atcgtcaggg
     5281 taagtttgtt cagagctggc tcgtgactgt cgggataact gaacgtgacc tcatcaagat
     5341 gcagtgcacc ggtgacaggg aggaccgaag aggacccgag gttctcagtg ccttctgtcg
     5401 ccagtagttc atcaatatgt ttgccgctgg ctgtcgcctg ctgccaggcg tcgacaatcc
     5461 ctgataactg ctgcactggc ccatagaatt gccccagcaa caggaagaaa gccgccagta
     5521 ccccggcagt catttctcca gcggcgacct gcgaagctcc cactaacaga acagcggcat
     5581 aggaggcctc ggtgcagaat gtcaggaacg gaaaatagac tgcaaggtat ttttgtgctc
     5641 gcacacgggt tgcgcggaaa cgctccgata acgcgcgcag ccgggcgccc tccagttcct
     5701 gctgaccatg cgattgcacg acacgcaggc cagagacttt ttcctgcagg gtgctgttga
     5761 ctttgcctat ttccagccgt gcctgtgcat aagcaggggt actgaggcgg cgataaatca
     5821 tggttgccag tgcggccact ggcactgcgc ttaatgccgt cagcgccagg aacgggtcga
     5881 gccagaacat ggtggcggcg attgcgaaca tcgtcaccag actggtggcc gcaccggcaa
     5941 ggccgttttg cagaaagcgg gcgagagagt ccacatcaac ggtcatgcgg gtaagacgcg
     6001 aatcggcatg cttttcatgc caggggagtc caagacgcag catatggccg aagctgcgca
     6061 agcgcaccga atgctgaact gattccgccg ctctggcggc gaagaccgtc tgcagagaat
     6121 aactgcacca gcccacaatg accaggcaca gggcgagcag ggcacagagg ccgatgatcg
     6181 acatatcgcc tgcggcgaca ccggagtcaa tgccgtgctg caacagtatc agtaccccta
     6241 cgcctgccgc agaatcgagt gcgatcagca gagcggcgat gacgaacatc cacgccacag
     6301 gcttcagtag tgacgtgact ttgcgacctt taccggctag cgcctgctgt actgcatttt
     6361 ccggtacgcg ggtcatacgg gcgacaaaac ctttatcccc cgtatccgtt acatcgtctt
     6421 gtgtcgctgg ttcagccggc cacaattcgt tgtggctatt gtgggacgtg gccagaaaat
     6481 cgctgtcgcc ggtcatcagt gcgcggaacg ccggacagcg cgcttcaagt tctgccgggg
     6541 ttccggtatc caccatacgg cccttatcca gcaccacaac ccggctggct agctgaagtg
     6601 ttgaacgccg tcgggcaatc accagcagca tatgcccttc gtcagcatag cgacccagcg
     6661 cggtattaat ctccgcttcc gtaacagcat caaccgctga ggtggtatca tccaggatta
     6721 acacgtccgg tgcagtaatg agcgcccgcg ccagcgctat tcgctgcctc tgcccgccgg
     6781 aaagattcgt accccgttcg gttaacaggc tatcgaagcc tttcggcagg gcgttgataa
     6841 aatcgctggc tcctgcagca gctgccgcac ggcgaatgtc gtccgccgtt gcctgagggt
     6901 ggccataggc gatattttcc gccaccgtac cggcaaacag aaaagcatct tcaaatacga
     6961 tgcctacccg gcgacgaagc gcctccagtc tgatatcgcg aagattttgt cggccttcgc
     7021 tggtgttgag ccatatcttt ccggagcagg gatcataaaa acgcgccagc agcatcaaca
     7081 gggtcgattt tcctgaaccc gatgcgccca ccaccgccac ggtttcaccg gcgcgcaggg
     7141 aaaaggagat attgctgagg atatgtctgt cgccatggtg gtagtcaaag ctcatattct
     7201 ccagttccag tccgacaacc tgactgttaa tcgactccgt cccgtcctcc atctgtgaac
     7261 gggtgtcgat cagtgcaaac acccgctcca cggacgcctg agtgcgctgt gcgataacca
     7321 gaaacgatgc cagtacgcgg gttggcccgg tcagcatcgc gaggaagctg gcgaatgcaa
     7381 cgaaggtacc gagatcgatc cgcccgtgca tgaccgacca cccgccgtag cacagcagga
     7441 cgatttgccc gagcaccggt agcgccagca tggtggcccc aggcatcgcc tgcgagataa
     7501 ccgcaccgat gcgtacacgc acaatctgcc gcgactgcct atcgagccag tgcgtttcac
     7561 gcatctctgc cacacaggat tttaccactg agatctgtgc cagcacctcg cgtatatgtt
     7621 cggtgagatt cgccagttgg tccgaggcca gcccggtttg cgcgaagacc ctacgacgcg
     7681 cacgaagcgc ggtgatcgcc aggcatacca gtacgcacac gacgataagc gtcatggcag
     7741 gggacatcca cagcatcacg gcaatgccgg caatgtaata agtgaacact gccagcggca
     7801 ccgggcacat ctgcagcagg gtatgcacct gctgcagatc gctgttggtc cgtgaaatca
     7861 cctgcccggt gcgcagcgca tcctggcctg cgccgtcaag tttctgaata ctgtcgaata
     7921 cccgtcctct gagggtatgc tgcacccaga gtgagagcat cccggcataa ccacggcgca
     7981 catagttacc gatgaaatca aaaaaagcga tcaacagtaa cccgcaggcc agccacggca
     8041 ggcgggccgg attgcccgcc agtgcgtcat tcacagcctg tcttgtcagc agtggcgtca
     8101 gcgcagccag caaaatagtc gatactgacg ctaccaccac gatgacacta agtttcttac
     8161 gttcccagca cacgcgcgcc aggcgaacta tccatgactg agccggtgtg ggagtgtgat
     8221 tcgcaggcat taagcctcct ccgtctgtaa aatacgatcc actggccgga tcgttccgca
     8281 aaaaagccag cacgctccgg tcccgcattg ttatggatgc atgactcctg gatgggtgct
     8341 ggcatatgat tatcatgaag gtgtagtggt taccctttct gtaccattgt gatcaggctt
     8401 ttcgccactt cacctgggca gggttgcgcc gccatttccg ctgctacctc ttcagaggct
     8461 ttgcgaagtg agcgattgtt gaggaaagca ttgatcatgt tactcgacag gccgacgtcg
     8521 ccagggataa tcccgcagcc gcgttcggca acaacgcggg catttaccgg gcgatcggct
     8581 ccctggccga agacgatttg tggaatacca gcgtgtagcg ctgtcagggt attgcctgct
     8641 ccgccatgat gaatgaaacc atctgcgccg ttgaggaaga cacccatggg tatccagtca
     8701 accaggcgaa catttgacgg cagcgagcgc aaatcggacc gcgcattggc tgaaatatgc
     8761 aggatgatct cggcatcgac ctcactggca gaatccataa cccaggcgat tagatcaagg
     8821 ccgtcaacca tcggtttgac agtgccgaga ctcaccagca gacgtttacg ctcgggcctt
     8881 ctctcccacc atggttccca caccgcgcca ccgttatacg ggacgtactg catcggaata
     8941 atgggttcac cgtcgttttc cagaatgctc atactcggcg gtgttacgtc gatccaggcc
     9001 atatcacgtg gtgtggcccc cacattgtgg cgacgatagg cgtccgtgag cgaacgcgtt
     9061 acacccttga tatgccaggg ggtatggccg aagcctacgg tttgcatcac gaccggtata
     9121 tcatatttgg cggcaatcag cggcccgatg actccgagcg gagggtagat gatgaggtca
     9181 ggccgccagt gtccggcaaa ctccaccaga tggtcggcca tttcttcgct gaagaatgag
     9241 aagttgccca tttgggttcc gatattactt tttttccgct gtgcctcatg gtggcggtaa
     9301 ccagcttcgg aatccagacc gggtgcggcg tcaaacacca ccaacccggc ctctgccgct
     9361 ttttgtgcga attgtcctcc actggcgatc agcacttcat gaccgttaac gcgaaatgcc
     9421 tgcgccagag acagcacagg atacaacagc ccatagagag gcgggcctac aaacaaaata
     9481 cgcatgagaa aatccctctc cgcttgacga tctaaatcag gcagtcgacg cactcatatg
     9541 ccagcagcag gacacgactt ttcgttaaac ctgctttacc agatgctgcg ccatttgtgc
     9601 ttcaccagcg ttatggacag ctcagtcaga gacttttccg tgacatacac aggtgaacag
     9661 ggaactccgt atccccactt ccattaacgc ttacgcgcca ttgtcctgtt gaacagccca
     9721 aatggcgtcg gacagctacg ccgggatgtt ctgacgcatt tgtgcagtgt tagaccgaac
     9781 aacgaatctg gaaagaaccg tgatttctta gacacacgat gcatcatcct gatagtctcg
     9841 ccttcgctgg tctgtaagta aaaacgccgt tgtacaggcg atgtttgttc cctttcagcc
     9901 ttaccagaaa caacacagac acaaatg
//
LOCUS       DAJOTB010000164         9850 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJOTB010000164 DAJOTB010000000
VERSION     DAJOTB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.164"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            214..1308
                     /gene="ddlA"
                     /locus_tag="OHN28_004092"
     CDS             214..1308
                     /gene="ddlA"
                     /locus_tag="OHN28_004092"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2299387.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1332..1544)
                     /locus_tag="OHN28_004093"
     CDS             complement(1332..1544)
                     /locus_tag="OHN28_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCQ2299388.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1804..2112
                     /locus_tag="OHN28_004094"
     CDS             1804..2112
                     /locus_tag="OHN28_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCQ2299389.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="OHN28_004095"
     CDS             complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="OHN28_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCQ2299390.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="OHN28_004096"
     CDS             complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="OHN28_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCQ2299391.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4850..6007
                     /gene="ampH"
                     /locus_tag="OHN28_004097"
     CDS             4850..6007
                     /gene="ampH"
                     /locus_tag="OHN28_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCQ2299392.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="OHN28_004098"
     CDS             complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="OHN28_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCQ2299393.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6717..9704)
                     /locus_tag="OHN28_004099"
     CDS             complement(6717..9704)
                     /locus_tag="OHN28_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2299394.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2548 a         2447 c         2307 g         2548 t
ORIGIN      
        1 actcaccaca tggtcattta agccaggaat aaattagctt cccgttcatc atttttgtga
       61 tcttgcacac atctttgaga taacgcactt tcttcaccag tacgttgctc gctttgtcag
      121 cagaagattg caacattctg tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      181 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      241 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      301 gccattgata aaagtcgctt cgatgttgtg ctgctgggca ttgataaaca agggcaatgg
      361 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg
      421 cgtccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      481 gcacaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      541 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt
      601 tctgatgttc tggcttcagc ggcctgtatg gataaagatg tcaccaaacg tctgctgcgc
      661 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc
      721 agttttgccg aagtggagtc taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag
      781 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacacaat tgccgtcgat
      841 ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgaaatc
      901 gaatgcgcag ttcttggcaa caataacccg caagcaagca cctgtggcga gatcgtactc
      961 accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1021 gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct
     1081 tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac
     1141 gaagtggtga tcaacgagat caacaccctg cctggcttca ccaacatcag tatgtatccg
     1201 aagctgtggc aagccagcgg tctgggttac accgacctga tcacgcgttt gattgagctg
     1261 gcgctggagc gtcacgctgc ggataacgca ctgaaaacca caatgtaata ttcaaacgcc
     1321 cggtaatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaatccc
     1381 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1441 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1501 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1561 aaaaacaaca tcttttcata ttctgtaaca actgtctgtg gtgtagacaa cactttcgca
     1621 attgttaata aatttaactt aaggttgcta ttttgtgact cgcaacgaag attcacgctt
     1681 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1741 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1801 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1861 tcaacgcctg gcaatattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggca
     1921 caattgctga acctgctagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     1981 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt tgggctgatc
     2041 ccgtttttag taggctgcct catttttgca gtagtggcgc tatggctgca ctggcgacat
     2101 cgccgccagt aagatttacg ttgccagatg cggcctaaaa cgccttatct ggcgtgacat
     2161 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2221 aggccgtagt cagattgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2281 cgcggggtag tgatttaccg gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2341 ccgtctcctc aaaagagaag ctgtcccctt tctctaactg acggcgaatt tcgctgtcac
     2401 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggctctttac
     2461 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg
     2521 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaaacggt
     2581 atatcggcgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2641 cgccgcggcg actgaagact tcctgacgga ctgtaccgtc cattttccac ggataccctt
     2701 tggtatgctg ttcggcaaaa gcaatactga cctgcatcgg cccaccggtg cgcaccggat
     2761 tgagcgaacc aaacagcgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg
     2821 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctatagctt ttgccgtttg
     2881 gcgatttgat ttttagcgcg gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac
     2941 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3001 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3061 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3121 tcgcaggcat cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca
     3181 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3241 aagatagagg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3301 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3361 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3421 caaaaacgtt agtaatctgc gtcatcaggc cgagtgtaat cgtaccggca acaattgacg
     3481 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatat
     3541 tgaaatacat atagtgaaaa tagaggcgga aatagttttt ccgtacggcg ctaaaaagct
     3601 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agttctttac
     3661 ggtaggcagc ctctacacgc tggtttttaa actccagccc cggcagtttg atccctacca
     3721 ctgccagtaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3781 gaatgtgccc gacaatcggc agctccggca catgtgcgga gagcgttacc agcaccggca
     3841 ggaaggcgat caacgtcatg atagcgttga taaagctgac gcccatattc tccagcgttg
     3901 aagcaaaacg catggtgtct tcctgcacac gctgcgcggc cccttcgata tgacgcagtt
     3961 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac
     4021 tgacaaagaa gttgttcagc acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4081 cgcccacttc gcggtaaaat tgttcgatgg tgactttatg cggcgaactt agcgccgttt
     4141 gaatcagatc atagaacggc gcataccagg cgttgacggc aaccccgact tccaccaaaa
     4201 accaggtgac gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg
     4261 ggctatagat aaaccagaac aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4321 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4381 cggtgatacg cgccacccaa tcacccccac cgacttgcca gaagataacg gcaatcaatg
     4441 cccaaacaaa ggccgagaga aaaaacattc ccggctttgg gaaaaaagac ttaaacatat
     4501 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4561 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4621 gcctgaccat aagaattgtt tcgacgggtt tcacaaacca aagattgttt acataagcgg
     4681 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4741 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4801 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4861 tctgcttttt tctgccgtgc tgtattcggc gtcattgacc tccgtccatg cggctcagcc
     4921 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     4981 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5041 cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt ccgtcatacg
     5101 tattgcttcc ctcaccaagt taatgaccag tgagatgctg gtaaaattgc tcgaccaggg
     5161 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac
     5221 ctacaatggg acgcccatta cgctggtgaa tctggcaacc cataccagcg ccctgccccg
     5281 tgaacagccc ggtggcgcgg cacatcgtcc ggtatttgtc tggccaacgc gcgagcaacg
     5341 ctggaaatac ctttccactg ccaaactaaa agccgcgccg ggcagccagg ccgcgtactc
     5401 caacctcgca ttcgatctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     5461 ccagttgttt gaagagcaga ttacccgtcc gttggggatg aaagacacca cctacacccc
     5521 ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa
     5581 cacgctggcg gcaattggca gcggcggcgt ctattccacg ccaggcgata tgatgcgctg
     5641 gatgcagcag tatttgtcat ctgattttta tcaacgcagt aaccaggctg atcgcatgca
     5701 gacgttgatt tatcagcgcg cgcagtttac aaaagtgatt ggcatggatg tccccggcaa
     5761 agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat
     5821 tattcagaag acaggtggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     5881 gaatattggt gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag
     5941 cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc
     6001 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6061 cgatatagcc gcctttacgc aaagcagcga gaacttccgc aacgacagaa cgcgatatac
     6121 gtgtacgttg atggatataa ttcatcacgc caatacgtga tcgcaattct tcattccagt
     6181 caatcattga taataacgtc gcgcggattt gttcgtagga attatgccca atgagctgaa
     6241 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6301 gttgattttg ttcaataaga gtaatggttt gtttggctgg caaatgatat cccgtacagt
     6361 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     6421 taatataagg agcctgtgta ataccgataa gtacgttttc ttctctacgc agagagataa
     6481 caccttctaa aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct
     6541 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     6601 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6661 aatccttgac gggcattatt tagattaaat tacctatttc ttcggagtga agaatattac
     6721 caggtatatt taacacccac attcgcggac cagtcttgat ctacgtcacc accaccgagg
     6781 tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca
     6841 acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg
     6901 ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg
     6961 ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac
     7021 tgaccatcaa ctttcatgtt gttgctcagc tggtaatcat caccagactg gaacagacca
     7081 gaaacgctgc cgtaaggagt tacgtaacct gcatcaccca gtttgaagtc gtaaccggct
     7141 ttcaaaccaa agccccaagc gtcggagttg gtgctgccgt caacgtaagt accgttgctc
     7201 atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccgtc aacaaagacg
     7261 ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga
     7321 ccagaacggt cattcatatc acctttagcg aagcctgcag ccgcaccgac gatccactta
     7381 gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca
     7441 tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg
     7501 ccgttatcag ccagaccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc
     7561 aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc
     7621 agctccatct gttgcagaac aacggtgtta ccacgctgct cagcctgata ggtgtatgca
     7681 cccaggtcag ctttgttagc agcagagaaa gtcgcgttgc tgtttttgtc gttgacgtag
     7741 ataatttctt tatctttgta atcagcgatt gcgccagcgc cagttgcgtt gtcgatacga
     7801 actttgtagt taccggttgc agcaacaacg ttgtctacag aactgttatc cagttcagta
     7861 ccgttgtcta cgtcgccgtt gccattgata gtcaggtgac catcagagtt catagcaaca
     7921 ataccgtaac cgtagttaga cttggaagta tcccacgtac ggtcgttcac caaatccgcg
     7981 ttcagaacgt aatcgctgct gttgatgtcg aacacaccag aaactacgtt gccgttatgg
     8041 tcagcgatgt tgccgctagt cagttccagt ttgtcagtac ggaaagcttc agtatcaaac
     8101 tgatcaacgt tcacatccag tacaccaccg ttagttacgg tgatggtatt ggcatacagg
     8161 ttagaatagt tattccagcc agtagattcg gttaaattaa cggtactgta gctatcaaca
     8221 gtcaggtggt ctgtagcaac caaaccatct tcaccaatat tcagtgagga gccgttggtt
     8281 aaaccaatgg tatctgagac cagagaggaa tcagtaacat ttacctgaga gccattattt
     8341 acagctaatg tatcaattac tgaactatta acggtattcc attctgaacc attgttcagt
     8401 gctacgttaa atacgccgct ctggaatacc ttgttgtcaa taatacgacc ccaatcgtta
     8461 gcttcgatag tggcaccagg ggtcaggctg ttcggcgcaa catcatacat atctgccgga
     8521 gcaattactt cagcactcat gtttgctgag ccaacccact tactaccgtt gttcagagta
     8581 atgttcagtt catcaacgtt ctgagcatca tctacccaac caccgttggt atcaaggaca
     8641 ccgtcaccat tgctgtcatg gccggttgaa tagaagaata cgccatcgtt attccagtgg
     8701 cttgtgaact cgacatcacc caacagggtt gaatggtcaa agtttacatt gtttttcatg
     8761 gaataatttg agccagcatt gtcagagaat gacagagcta cgtcattttc gccaccatta
     8821 ccagcgtaat cacttggttc agcagattta ccgaagaagc cattgctttc ttccagcgga
     8881 gtctgtgacc ctgaagttac agtggagtta gacacattaa tgttgttgtc ccactgatga
     8941 tattcgctag tataagtcgt gttgcttgta ttgccttcag acagcgcaat accagctaca
     9001 cgggagttgt tagtaatatt gatattgctt tcaacatcaa gatttactgc aacacccaga
     9061 gcggtataga acgtttcatt ggttgactta gatttatcac catcaagata tgtgtcagtg
     9121 aagtaaagag cttcatagtc atcatcaatg gtggaattgg atacgttcag agtgaatacg
     9181 tcaccatcgt accaattgta gtcaaggtca gtagtgattc cgttatagaa attatagcca
     9241 ctataggtct gtggagttac aacttcacct ttagcattaa cttctgcagg agtaatgctg
     9301 taataaccaa atggcagttc agaagtgatt gacccatgaa ttactgaatt gctgatatct
     9361 aactggttaa cagaaccgct agcgtaatca tgattcaagt aataagtaga aatggtaccg
     9421 ttaacagtat cgcctttaaa gacggtgtcg aaatcaccat tggcattgtt atagtaaaca
     9481 aaaccattgt aagttacgta gttaccgtct gaacccatcg ggtccatgcc gccagcatcg
     9541 gcaattccat cattgtcagt gtcatacggc caaactaaat cggtcgtggt gttgtcatga
     9601 tacccccagg tatcatagtt tgctgcatta gcctgagagg tgatagccag agtgcaagcc
     9661 aatgctaatt gtgatactac aagtttcttt ttccaggagt gcatttgtca tccctcctca
     9721 gggacgtaat aagttgatcc atcaattatc aatgcataga aaacatatta tgtttcgcgt
     9781 ttacgattat gcggcgttat agaggaatgg ttcaattatt ctttccccta agtccgattg
     9841 cggacatata
//
LOCUS       DAJOTB010000165         9847 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJOTB010000165 DAJOTB010000000
VERSION     DAJOTB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.165"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..404)
                     /gene="folK"
                     /locus_tag="OHN28_004100"
     CDS             complement(<1..404)
                     /gene="folK"
                     /locus_tag="OHN28_004100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCQ2299395.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAP"
     gene            complement(401..1819)
                     /gene="pcnB"
                     /locus_tag="OHN28_004101"
     CDS             complement(401..1819)
                     /gene="pcnB"
                     /locus_tag="OHN28_004101"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCQ2299396.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1858..2784)
                     /gene="gluQRS"
                     /locus_tag="OHN28_004102"
     CDS             complement(1858..2784)
                     /gene="gluQRS"
                     /locus_tag="OHN28_004102"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCQ2299397.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQNIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2821..3276)
                     /gene="dksA"
                     /locus_tag="OHN28_004103"
     CDS             complement(2821..3276)
                     /gene="dksA"
                     /locus_tag="OHN28_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCQ2299398.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3454..4158)
                     /gene="sfsA"
                     /locus_tag="OHN28_004104"
     CDS             complement(3454..4158)
                     /gene="sfsA"
                     /locus_tag="OHN28_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCQ2299399.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4173..4703)
                     /gene="thpR"
                     /locus_tag="OHN28_004105"
     CDS             complement(4173..4703)
                     /gene="thpR"
                     /locus_tag="OHN28_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCQ2299400.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4777..7206
                     /gene="hrpB"
                     /locus_tag="OHN28_004106"
     CDS             4777..7206
                     /gene="hrpB"
                     /locus_tag="OHN28_004106"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCQ2299401.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFLRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPRLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7300..9825
                     /gene="mrcB"
                     /locus_tag="OHN28_004107"
     CDS             7300..9825
                     /gene="mrcB"
                     /locus_tag="OHN28_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCQ2299402.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
BASE COUNT         2256 a         2512 c         2815 g         2264 t
ORIGIN      
        1 tccggcgcaa tttcaaacag tggccacagc ataaagccac gatttttcat atcgtagtgc
       61 ggaacggtca gacgttcggt atttattact tcattaccaa acagcatgat gtcgagatcc
      121 agcgtgcgcg gtccccagcg ttcagcttta cggacgcgac cttgctgcaa ttcgatacgc
      181 tgtgtgtgat tgagtagctc ttcaggggca agagtggttt tcagcgcgac ggcggcattt
      241 aagtaatcgg gttgatcttg cggccccaga ggtggggtgc ggtaaaacga agaaacggca
      301 agaatgcggc tttctggaat atcgcctaat gctttcaggg cagcattgac ctgctccagc
      361 ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgtagta ccctcacgac
      421 gcggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg tgacggttct tcatccagtt
      481 cgttaagcat ccctttttgg tctggcggcg cggaaacctg gaactcaccc caccatttca
      541 ccagacgttg cagttcagcg ttacgctcaa cttcggctcg taaggccaac aggtcgtatg
      601 ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg tttaccctga cgacgggaca
      661 tacgcaactg caactgccag atatcgcgag ttagcgtagt cagacgtttc gggatcgcca
      721 gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag tgcgaaagcg tcgtgatagg
      781 tcaggccgct ctcctgagca attttctgtg ccgtctccag caatgggtac cagaacatgg
      841 cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct
      901 tcagtacctg ctcaataatg cgttccatcg ggctgtcgcc attttccgtg aagtagcggg
      961 taatggtcgg gaacagcggc tggaacagat gatattcaca cagcagctta taggtttcgt
     1021 aaccgtagcc cgcttgcaac agtttcagcg attcttcaaa cagacgtgcc ggtgggatat
     1081 cgttcagtaa ggttgccagg cgtgggatcg gctcggcggt ttccgggctg atgcgcatgc
     1141 ccaatttggc ggcaaaacga accgcgcgga gcatacgtac cggatcttca cggtagcgcg
     1201 tttccgggtt accaatcaga cggataacgc cgtctttcag atccttcatg ccgccaacgt
     1261 aatcacggac ggtaaaatct gccacgctgt aatacaggct gttgatagtg aaatcgcggc
     1321 gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacata ccgttttgcc
     1381 cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg atgtccacgg aaggtcgcaa
     1441 cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga cccaccagac
     1501 ggcagttacg gaacagttta cgtacctgtt caggcgtggc gttagtggtt acgtcaaaat
     1561 ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt
     1621 atcccgcttt attgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg
     1681 aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag
     1741 cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag
     1801 tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca
     1861 gcatgacgca tttgagaatg ttgaatttac aattgccgac tcaggcactg cggtgagccg
     1921 ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgtttc
     1981 tacctgctga cccagaaaat gaagtgccgc gattaacacc gggcgcggat cgcctttcgg
     2041 caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg
     2101 taaatggatg taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccgcac
     2161 agtcggttcg atcaaatctg ccccgcggac aatttcgctc acgccctgga agtgatcatc
     2221 caccaccacc gccaggttat aggcaaacaa accatcacgg cgatgaataa taaaatcttc
     2281 ccgtgccagt ttttcgtcgg cgtgaataat gcctcgcagc aggtcagtaa actgggtgac
     2341 tggatgctgc tggcggatac gtactgcggc gttgtctggt ccatgatgca aatcccggca
     2401 atgaccgtcg taaataccgc caatgttttg aatacgcgca cgcgtgcagg tgcagtaata
     2461 acttagtcct tgctcatgta accaggcgag tgcttcgcga taggcgtggt gacgttgcga
     2521 ttgccagaga acgtcgccgt cccagtgtag gccgtaatgt tccagctggc gcaggatagt
     2581 ctctgcggca ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta ccagccagcg
     2641 accttgccgg gcgcgagcct gcaaatagct gccaagcgca gcaatcagag aaccaaaatg
     2701 aagctcgccg gaaggagagg gggcgaagcg accaatatag tgtgtgtctg tcatctcttt
     2761 gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acatgatgga acggctgtaa
     2821 ttagccagcc atctgttttt cgcgaatttc agccagcgtc ttgcagtcga tgcatagatc
     2881 ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta
     2941 gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg
     3001 ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac
     3061 cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc
     3121 cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt
     3181 catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact
     3241 cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta
     3301 tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat
     3361 agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg
     3421 accagaatgt aaattaacca gttacttact ctactacaat gtaaccggta gtgatttttt
     3481 aagagccatg ccttcggcag aaagttccgc tttgtaagcc agaatttcta cccccctctg
     3541 ttgagcttct gacaatagtt gtgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg
     3601 tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac
     3661 gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc
     3721 ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc
     3781 cgcctgcaac ataaagtcaa tacggctgcg ttcggcgccg tattttactt cgcttttcag
     3841 cgagctatag cctgacagct ctgaaattga ttcattaagg atagcctctt tcgtcaacct
     3901 gttagcccaa agcgtattga cgcaaataat tgcgccgctt tggctttgag ttaattccca
     3961 ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg
     4021 cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc
     4081 gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag
     4141 agggggagaa aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc
     4201 ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc
     4261 gtatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat
     4321 aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt
     4381 gaacggagca tattcgccag ctggattaag ccgcgtggcg gctgtcgcat ccctaaccac
     4441 accacacgcg aacgcagcca ttgaccggcg tcatcaagcg tgagtgtgaa accaggttga
     4501 cgaatccgtc cggctaaaag agaaagcgcc ttctctttct ctgcgctcac ttcgcctaaa
     4561 aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag
     4621 tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag
     4681 aacagccgtt gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa
     4741 tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc
     4801 gtcttacctg agttacttgc tgccctcgat ggtgcatcgc aggtgttatt aagtgcgccg
     4861 accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac
     4921 gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgttgc gcaacgactg
     4981 gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac
     5041 tgcgtcgggc cgaatacccg cctggaagtg gttaccgaag gcgtgctgac gcggatgatc
     5101 cagcgtgacc cggaactgag cggtgttgga ttggtgatcc ttgatgaatt tcatgagcgc
     5161 agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat
     5221 gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg
     5281 ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cagttgaacg ccgttatttg
     5341 ccgctgcccg ctcatcagcg ttttgacgaa gccgtcgcgg ttgccaccgc cgaaatgctg
     5401 cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg
     5461 caggaacaac tgacttcacg catcggcagt gatgtgttgc tctgcccgct gtatggcgcg
     5521 ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgtaaagtg
     5581 gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg aaggtattcg tctggtggtg
     5641 gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgta cagggcttac gcggctggtt
     5701 actcaacgtg ttagtcaggc gtcgatgacg caacgtgccg ggcgtgccgg gcgtctggag
     5761 ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc cgcgcaaagt
     5821 gaaccggaaa tattacaaag cgatctttcc tgtttgctga tggaattact gcaatgggga
     5881 tgcagcgatc cggcgcagat gagctggctg gatcaaccgc caacggtgaa tctactggcc
     5941 gcgaaacgcc tgttacggat gctgggggcg ctggacggtg aacggcttag tgcgcaaggg
     6001 caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag
     6061 aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg
     6121 atgggcaata gtgacctggg cgtggcgttt ttgcgcaatc aacccgcctg gcagcaacgt
     6181 agtcagcaac tgttaaaacg tttaaacgta cgcggcggtg aggcagacag ttcgcttatc
     6241 gcgccgcgac ttgccggagc gtatgccgat cgtattgctc gccgccgtgg gcaagaggga
     6301 cgctatcaac tggcgaacgg catgggggcg atgctcgatg ccgacgatgc gcttagccgc
     6361 cacgaatggt tgatcgcgcc gttattattg cagggcagtt cttcgccgga tgcgcggatt
     6421 ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca gctggtacag
     6481 cagtctgaca ccgtggagtg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg
     6541 caaatcggtc agttgacggt aaaagtgcaa ccgctggcga aaccctcgga agacgagttg
     6601 catcaggcga tgcttaacgg cattcgtgat aaaggtttaa gcgtgctcaa ctggacggcg
     6661 gaagcggaac agctacgctt gcgtttgtta tgtgccgcaa agtggttgcc ggaatatgac
     6721 tggccagcgg ttgatgatga aagtttgttg gcgacgctgg aaacgtggct actgccgcat
     6781 atgacaggcg tacattcgct acgcggcctg aaatcactcg atatttatca ggcactgcgt
     6841 ggattacttg attgggtaat gcagcaacgt ctggatagtg aattgcctgc gcattacact
     6901 gtgccgacgg gaagccggat cgccattcgt taccatgaag ataacccgcc cgcgctggcg
     6961 gtgagaatgc aggagatgtt tggtgaggcc tctaatccga ctatcgccca ggggcgcgta
     7021 ccgttggtgc tggagttgct ttcccctgcc caaaggccgc tgcaaatcac gcgtgatttg
     7081 agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc
     7141 aaacatgtct ggccggacga cccggcaaac accgcaccga cgcgacgaac gaaaaagtat
     7201 tcgtgaagac caaagcaaat ttgagagata tcttcttctg tcttgcaaca gaagaacaga
     7261 aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag
     7321 ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt
     7381 tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg
     7441 cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg
     7501 ctgctgctaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc
     7561 gatcaaaaaa ttcgcagccg tattgatggc aaggtctggc aattgcctgc ggcagtttat
     7621 ggtcgaatgg tcaaccttga gccagacatg accatcagca agaacgagat ggtgaagctg
     7681 ctggaggcga cccagtatcg ccaggtgtcg aaaatgaccc gtcctggtga gtttaccgta
     7741 caggccaaca gcattgagat gatccgccgt ccgtttgatt tcccggacag caaagaaggg
     7801 caggtgcgcg cgcgtctgac ctttgatggc gatcatctgg cgacgatagt caatatggag
     7861 aacaaccgtc agttcggttt cttccgtctt gatccgcgcc tgatcaccat gatctcttcg
     7921 ccaaacggtg agcagcgtct gtttgtgccg cgcagtggtt tcccggattt gctggtggat
     7981 actttgctgg cgacagaaga ccgtcatttc tacgagcatg atgggatcag tctctactca
     8041 atcggacgtg cggtgctggc aaacctgact gccggacgca cggtgcaggg ggcgagtacg
     8101 ctgacgcaac agttggttaa aaacctgttt ctctccagcg agcgttctta ctggcgtaaa
     8161 gcgaacgaag cctacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt
     8221 gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc
     8281 ccgctggcga gcttgtatta ctttggtcgc ccggtagaag aattgagcct cgatcagcag
     8341 gcgctgttgg tgggtatggt gaaaggggcg tccatctaca acccgtggcg taacccgaaa
     8401 ctggcgctgg agcgacgtaa cctggtacta cgtttgctgc aacagcaaca aattattgat
     8461 caagaactct atgacatgtt gagtgcacgt ccgctgggtg ttcagccgcg cggtggggtg
     8521 atctctcctc agccagcctt tatgcagctg gtgcgtcagg agctgcaggc gaaattaggc
     8581 gataaggtaa aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag
     8641 gacgcggcag aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg
     8701 agcgatcttg aaaccgcgat tgtggttgtt gaccgcttta gcggtgaagt tcgtgcgatg
     8761 gtcggtggct ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg
     8821 attggttccc ttgcaaaacc agcgacttat ctaacggcct taagccagcc gaaaatctat
     8881 cgtttgaata cgtggattgc cgatgcgcca atcgcgctgc gtcagccgaa tggccaggtc
     8941 tggtcaccgc agaacgatga tcgtcgttat agcgaaagtg gcagggtgat gctggtagat
     9001 gcgttgaccc gttcgatgaa cgtgccaacg gtgaatctgg gcatggcgtt ggggctgcct
     9061 gcggttacgg atacctggat caaactgggt gtgccgaaag atcaactgaa tccagttcca
     9121 gcaatgttgt tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc cttccagact
     9181 atcgccagcg gtggtaatcg tgctccgctt tccgcgctac gttcagtcat cgcagaagac
     9241 ggcaaggtgt tgtatcagag cttcccgcag gcagaacgcg ctgttccggc gcaggcggcg
     9301 tatctgacac tatggaccat gcagcaggtt gtacaacgcg gtacgggacg tcagcttggg
     9361 gcaaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat
     9421 acctggtttg cgggcattga tgggagcacg gtgaccatca cctgggtcgg ccgtgataac
     9481 aaccagccga ccaaactgta tggtgccagc ggggcaatgt cgatttatca gcgttatctg
     9541 gctaaccaga cgccaacacc gctgaatctt gttccgccgg aagatattgc agatatgggc
     9601 gtggactacg acggcaactt tgtttgcagc ggtggtatgc gtgtcttgcc agtctggacc
     9661 agcgatccgc aatcgctgtg ccagcagagt gagatgcagc agcaaccgtc aggcaatccg
     9721 tttgatcagt cttctcagcc gcagcaacag caacctgctc aacaggagca aaaagacagc
     9781 gacggcgtgg ctggctggat caaggagatg tttggtagta attaatatcg taatcgctct
     9841 ccgctta
//
LOCUS       DAJOTB010000166         9694 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJOTB010000166 DAJOTB010000000
VERSION     DAJOTB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.166"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            136..1113
                     /gene="csiD"
                     /locus_tag="OHN28_004108"
     CDS             136..1113
                     /gene="csiD"
                     /locus_tag="OHN28_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCQ2299403.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1133..2398
                     /gene="lhgO"
                     /locus_tag="OHN28_004109"
     CDS             1133..2398
                     /gene="lhgO"
                     /locus_tag="OHN28_004109"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCQ2299404.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            2424..3872
                     /gene="gabD"
                     /locus_tag="OHN28_004110"
     CDS             2424..3872
                     /gene="gabD"
                     /locus_tag="OHN28_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2299405.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3886..5166
                     /gene="gabT"
                     /locus_tag="OHN28_004111"
     CDS             3886..5166
                     /gene="gabT"
                     /locus_tag="OHN28_004111"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCQ2299406.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5403..6803
                     /gene="gabP"
                     /locus_tag="OHN28_004112"
     CDS             5403..6803
                     /gene="gabP"
                     /locus_tag="OHN28_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCQ2299407.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6824..7486
                     /gene="csiR"
                     /locus_tag="OHN28_004113"
     CDS             6824..7486
                     /gene="csiR"
                     /locus_tag="OHN28_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCQ2299408.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7487..7936)
                     /gene="kbp"
                     /locus_tag="OHN28_004114"
     CDS             complement(7487..7936)
                     /gene="kbp"
                     /locus_tag="OHN28_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCQ2299409.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8020..8178)
                     /locus_tag="OHN28_004115"
     CDS             complement(8020..8178)
                     /locus_tag="OHN28_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCQ2299410.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8361..8660
                     /locus_tag="OHN28_004116"
     CDS             8361..8660
                     /locus_tag="OHN28_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2299411.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8670..9194
                     /gene="ygaP"
                     /locus_tag="OHN28_004117"
     CDS             8670..9194
                     /gene="ygaP"
                     /locus_tag="OHN28_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCQ2299412.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9241..9645)
                     /gene="stpA"
                     /locus_tag="OHN28_004118"
     CDS             complement(9241..9645)
                     /gene="stpA"
                     /locus_tag="OHN28_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCQ2299413.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2222 a         2508 c         2655 g         2309 t
ORIGIN      
        1 tgctgttttt gatcacaata agaaaacaat atgtaacttt tatgcgcatt tttcagaaat
       61 gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct acatctcata
      121 agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt cgattcaggc
      181 caggactatc gcggattcac tctcatcccg tcggcgcaat ccccgcgtct gctggaactc
      241 accttcaccg aacagacgac caaacagttt ctcgaacagg ttgccgaatg gcccgtgcag
      301 gcgctggagt acaaatcgtt tctgcgtttt caggtaggca aaattctcga cgatctgtgt
      361 gcgaatcagc tgcaaccgct gctgttgaag accctgttaa accgcgctga aggtgcgctg
      421 ttgatcaatg cggtgggtat cgatgatgtc gcgcaggcgg atgagatggt gaagctggcg
      481 acggcggtgg cgcatctgat tggccgttcc aatttcgatg ccatgagcgg tcagtattac
      541 gcgcgtttcg tggtgaaaaa tgttgataac tcagacagct atcttcgtca gccgcaccgc
      601 gtaatggagc tgcacaacga cggcacttac gtcgaagaga tcactgatta cgtgctgatg
      661 atgaaaatcg acgagcaaaa catacagggc ggtaactcgc tgctgctgca tctcgatgac
      721 tgggaacatc tggacctctt tttccgccat ccgctggcgc gccgtccgat gcgctttgcc
      781 gcgccgccga gcaaaaacgt cagcaaagat gtcttccatc cggtgttcga tgtcgatcag
      841 cagggccgcc cagtgatgcg ctatatcgac cagtttgtcc agccgaaaga cttcgaagaa
      901 ggcgtgtggt tgagcgagct ttcggacgcc attgaaacca gtaaaggcac tctttctgta
      961 cccgttcccg ttggcaaatt cctgttgatt aacaacctgt tctggctgca cggtcgcgac
     1021 cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct
     1081 tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc ggatgtatga
     1141 ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac tgattgatat
     1201 ctatcctgac gcccgcattg cgttgctgga aaaagagtcc ggccctgcct gtcaccagac
     1261 gggccacaac agcggcgtga tccatgccgg agtctattac acgcccggta gcctgaagac
     1321 gcagttttgc ctggcgggga accgcgccac taaagtcttt tgcgatcaaa acggcattcg
     1381 ctacgacaac tgcggcaaga tgctggtcgc cacgtctgaa ctcgaaatgg aacggatgaa
     1441 tgcgttatgg aaacgcacgg cggcaaacgg gatcgagcgc gagtggttaa acgccgagga
     1501 gctgcgcgag cgtgaaccga atatcaccgg gcttggcggc atttttgtgc cgtccagcgg
     1561 cattgtcagc taccgcgaag tgacggcggc gatggcaaaa atcttccagg ccagaggtgg
     1621 tgagattatt tataacgccg aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat
     1681 acgtacccgt cagggcggtg aatatgaagc atcgacgctg attagttgtt ccgggctgat
     1741 ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggc tttattatct gcccgttccg
     1801 cggcgagtat ttccgccttg cgccagagca taaccagatt gttaaccacc tgatttaccc
     1861 cattcccgac ccggcaatgc cgtttttggg cgtgcatctc acccgcatga tcgacggcag
     1921 cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctacc gcaagcgcga
     1981 cttctcattt agtgacacgc tggagatttt aggctcgtcg gggattcgcc gggtgctgca
     2041 aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct
     2101 gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc cctggccccc
     2161 cggtgtgcgg gcgcaggcgg tatcgccgga cggcaagctg attgacgatt ttctgtttgt
     2221 caccaccccg cgcacgatcc acacctgtaa tgcgccctcc ccggcagcga catcagcaat
     2281 tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc
     2341 cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat ttaattaata
     2401 acctttgaaa acaggatgta gcgatgaaac ttaacgacag taacttattc cgccagcagg
     2461 cgttgattaa cggggaatgg ctggacgcca acaatggcga ggtcatcgac gtcaccaatc
     2521 cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg
     2581 ctatcgacgc cgccagccgc gccctgcccg cctggcgtgc gctcactgcc aaagaacgcg
     2641 ccaacattct gcgcaactgg ttcaatttaa tgatggagca tcaggacgat ttagcgcgcc
     2701 tgatgaccct cgaacagggc aaaccactgg ctgaagcgaa aggcgaaatc agctacgccg
     2761 cctcatttat tgagtggttt gctgaagaag gcaaacgtat ttatggcgac accattcccg
     2821 gtcatcaggc cgataaacgc ctgattgtga tcaagcagcc gattggcgtc accgcggcta
     2881 tcacgccatg gaacttcccg gcggcgatga ttacccgtaa agctggtccg gcgctggcgg
     2941 ctggctgcac catggtgctg aagcccgcca gtcagacgcc gttctctgcg ctggcgctgg
     3001 cagaactggc gattcgcgcg ggcgttccgg ctggcgtgtt taacgtggtc accggttcgg
     3061 cgggcgcggt cggtaacgaa ctgaccagca acccgctggt gcgcaagctg tcgtttaccg
     3121 gctcgaccga aattggccgt cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt
     3181 cgctggagct gggcggcaac gcaccgttta ttgtctttga cgatgccgat ctcgataaag
     3241 ccgtggaagg cgcgctggcc tcgaaattcc gtaacgccgg gcaaacctgc gtctgcgcca
     3301 accgcctgta tgtgcaggac agcgtgtatg accgctttgc cgaaaaattg cagcaggcgg
     3361 tgagcaaact gcacatcggc gacgggctgg agaaaggcgt caccatcggg ccgttgatcg
     3421 atgaaaaagc ggtagcaaaa gtggaagagc atattgctga tgcgctggag aaaggcgcgc
     3481 gcgtggtttg cggcggtaaa gcacatgaac gcggcggtaa cttcttccag ccgaccattc
     3541 tggtagacgt tccggctaac gccaaagtgt cgaaagaaga gacgtttggc cccctcgccc
     3601 cgctgttccg ttttaaagat gaagccgatg tgattgcgca agccaatgac accgaatttg
     3661 gacttgcagc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcac
     3721 tggagtacgg catcatcggc atcaacaccg gcattatttc caatgaagtg gccccgttcg
     3781 gcggcattaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc gaagattact
     3841 tagaaatcaa atatatgtgc atcggtcttt aactggagaa tgcgaatgat cagcaataaa
     3901 gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt
     3961 ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga gtatcttgat
     4021 ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc atccgaaagt ggttgcggcg
     4081 gtggaagcgc aattgaaaaa actgtcgcac acctgcttcc aggtgctggc ctacgagccg
     4141 tatctggagc tgtgcgagat tatgaatcag aaggtgccgg gcgattttgc caagaaaact
     4201 ctgctggtta cgaccggttc cgaagcggtg gaaaatgcgg tgaaaatcgc ccgcgccgcc
     4261 actaaacgta gcggtactat cgcctttagc ggtgcgtatc acgggcgcac gcattacacg
     4321 ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat gccgggtcat
     4381 gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga cgctatcgcc
     4441 agcatccacc ggatcttcaa aaatgacgcc gcgccggaag atatcgccgc catcgtgatt
     4501 gagcccgttc agggcgaagg cggtttctac gccgcgacgc cagcctttat gcagcgttta
     4561 cgcgcgctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca gagcggcgca
     4621 gggcgtaccg gcacgctgtt tgcgatggag caaatgggcg tggcaccaga tctcaccacc
     4681 tttgcgaaat cgatcgcagg cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg
     4741 atggacgccg tcgcaccggg cggactgggt ggcacctatg ccggtaaccc gattgcctgc
     4801 gtagcggcgc tggaagtgtt gaaggtgttc gagcaggaaa atctgctgca aaaagccaac
     4861 gttctggggc agaagctgaa agacggattg ctggcgatag ccgaaaaaca cccggagatc
     4921 ggcgacgtac gcggtctggg ggcgatgatc gccatcgagc tgtttgaaga tggcgatcac
     4981 agcaaaccgg acgcaaaact caccgccgag atcgtggcac gcgcccgcga taagggtctg
     5041 attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc gctcaccatt
     5101 gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc tgaggcgaag
     5161 ctgtagcacc gcaccatgcc ggaggcgacg ctttgcggct tgtccggcct acgagaatcc
     5221 aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta
     5281 tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga accaataaca attaaacgcg
     5341 tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
     5401 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaagtca cgccacgtca
     5461 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
     5521 tcgccgaagc gggcccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta
     5581 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
     5641 cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg ttgtactggt
     5701 ggttttgggt actggttatc ccacttgaag ccaacatcgc cgccatgatc ctgcactcat
     5761 gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaaccggca
     5821 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgtaaag
     5881 tcatcgctat cctggccttt attttccttg gtgccgtcgc aattagcggt ttttacccgt
     5941 atgccgacgt gagcgggatt tcaagattgt gggatagcgg cggctttatg cccaacggct
     6001 tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
     6061 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgttcgc gccaccaact
     6121 cggttatctg gcgtatctct attttctatt tgtgttctat ttttgtcgtt gttgcattaa
     6181 tcccgtggaa tatgccaggg ctgaaagccg ttggttctta tcgctcggtt ctggaattgc
     6241 tcaatattcc tcatgcgaaa ttaatcatgg actgcgtgat attactttcc gtaaccagtt
     6301 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggtg
     6361 atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgta gcggtgttac
     6421 tctccaccag cgcggcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtgt
     6481 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
     6541 tttcacaatt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcctgcgga
     6601 tgtggcttta tccgtggctc acctggctgg tcatcggctt tattaccttt gtgttggtag
     6661 tgatgctatt ccgcccggcg caacagttag aagtgatctc caccggctta ttagcgattg
     6721 ggattatctg taccgtgcca attatggctc gctggaaaaa gctgataatg tggcaaaaaa
     6781 cacccattca taatacgcgc tgataatgat caggagtcac accatgacca ttacctctct
     6841 ggatggctat cgctggctga agaacgatat tattcgcggc aattttcagc cggatgaaaa
     6901 attacggatg agtttgctga catcgcgtta tgcacttggc gttgggccgt tgcgggaagc
     6961 actttcgcaa ctggtggcag aacgactggt cacggtggtg aatcaaaaag ggtatcgggt
     7021 ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc
     7081 gatgctggtg agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc
     7141 aaaagcgcat ctgctcagta agcttgaggc ctgtgacgcc agtgagaaaa tgcttgatga
     7201 gtgggatctg cgtcatcagg cgtttcatac tgcaattgtg gcgggctgtg gttcttacta
     7261 tttgctgcaa atgcgtgaac ggttgtttga tctggcagcg cgttatcgat ttatctggct
     7321 gcggcgaaca gtgctttcgg tggaaatgct ggaagataaa cacgatcagc accagaccct
     7381 gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgc gccagcattt
     7441 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttcaggaa
     7501 tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt ttattcgctt
     7561 cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag
     7621 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
     7681 gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
     7741 cctcctgact taagccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
     7801 ctttatcggc atcaggtata ccggttttgt tcagatgctc ctgcaccttc ttcgcctgat
     7861 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
     7921 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
     7981 ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca
     8041 gaacgcgtga atcaaaccgg ggatatagcc aagcagtgtc agcagaatat taataatgaa
     8101 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
     8161 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
     8221 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagcgt acgcttttac
     8281 tctcatttac gctgctgatg ctggtatatg ttgcacactt agtttagagt ttacttaatt
     8341 tagaaaatac ttaaacaact atgactgaac tcgcacaatt acaggccagt gccgaacagg
     8401 cagcggcctt attgaaatca atgagccacc ctaaacgatt gctgattctg tgtatgctta
     8461 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
     8521 cgacatcaca gcatctcgct cgtatgcggg gcgaagggct tatcgactgc caacgggatg
     8581 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
     8641 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
     8701 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgatgct gatgaatatc
     8761 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtactggaa cagtcaggtc
     8821 ttccgcctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
     8881 gtaataacgc tgataaatta gcagcgattg ccgcccccgc agaaatcttt ttactcgaag
     8941 atgggattga tggctggaaa aaagcaggat tgccagtagc ggtaaataaa tcccaaccct
     9001 taccgttaat gcgccaggtg caaatcgccg cgggtggttt aatattaatc ggcgttgtac
     9061 tgggttatac cgtaaatagc gttttcttct tattaagcgg ctttgttggt gctgggttac
     9121 tgcttgcagg aattagtggt ttttgtggaa tggcaaggtt gttagataag atgccgtgga
     9181 accaacgagc ttgagcagcg acgccggacg cgccatagca gcgacatccg gcctcagtaa
     9241 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcga ttggcttcgg
     9301 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcgatga atttatattt
     9361 cgccggacgc ggctggcgtt ttttaccagc gcgtggtgcc gcagcagagc tattacccaa
     9421 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
     9481 ctggcgctct gccagttcac gctgctgttg ttcttcttct tcacgtcttt ctttagtgac
     9541 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
     9601 acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
     9661 ttaacgccaa aacaaaaaag tatttcgtcg cgta
//
LOCUS       DAJOTB010000167         9693 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJOTB010000167 DAJOTB010000000
VERSION     DAJOTB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.167"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(4..3528)
                     /gene="nifJ"
                     /locus_tag="OHN28_004119"
     CDS             complement(4..3528)
                     /gene="nifJ"
                     /locus_tag="OHN28_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCQ2299414.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3802..4068
                     /locus_tag="OHN28_004120"
     CDS             3802..4068
                     /locus_tag="OHN28_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCQ2299415.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4065..4487)
                     /gene="hslJ"
                     /locus_tag="OHN28_004121"
     CDS             complement(4065..4487)
                     /gene="hslJ"
                     /locus_tag="OHN28_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCQ2299416.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4598..5587)
                     /gene="ldhA"
                     /locus_tag="OHN28_004122"
     CDS             complement(4598..5587)
                     /gene="ldhA"
                     /locus_tag="OHN28_004122"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2299417.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5795..8434
                     /locus_tag="OHN28_004123"
     CDS             5795..8434
                     /locus_tag="OHN28_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCQ2299418.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            8431..8616
                     /locus_tag="OHN28_004124"
     CDS             8431..8616
                     /locus_tag="OHN28_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCQ2299419.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            8624..8950
                     /locus_tag="OHN28_004125"
     CDS             8624..8950
                     /locus_tag="OHN28_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCQ2299420.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(9122..9364)
                     /locus_tag="OHN28_004126"
                     /pseudo
     CDS             complement(9122..9364)
                     /locus_tag="OHN28_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9372..9693
                     /locus_tag="OHN28_004127"
                     /pseudo
     CDS             9372..9693
                     /locus_tag="OHN28_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="involved in the synthesis of a cofactor required
                     for carnitine dehydratase and carnitine racemase
                     activities; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
BASE COUNT         2379 a         2373 c         2541 g         2400 t
ORIGIN      
        1 catttaatcg gtgttgcttt tttccgcttt tccggccatt tgtgccagga agtcatagcg
       61 tttttgcaaa tcagctgcag catctttcca taactgttct gccacttctg gctgctgcga
      121 attcagacga cggaaacgct gctcgtgaag taacgtttct tcaagtgctt ctgacggcgg
      181 gcgtgaatcc agcgccagcg gcagtttgcc ttcatcggca cgacgaggat cgaagcgata
      241 taacggccag aagccggtag cagtgagttg gcgcatctga tcgtggctga gtgccagatc
      301 gtaaccatgc tcttcacacg ggctataagc gatgatcagc gatggccccg gatacgcttc
      361 cgcttcctga atcgctttca ccgtctggtt cagctgcgcg ccgagagaaa tctgcgccac
      421 ataaacatga ccgtacatca tcatactgac gccaagatct ttacgcgctt tacgtttgcc
      481 atgctcgcca aacttagtta ctgcacccag cggtgtcgct ttcgatgcct gacctccggt
      541 gttggaatag cattgcgtat ccagcaccag aatgttgacg ttttccgtca aactcaatac
      601 atgatccaga ccgccaaagc cgatatcgta agcccagcca tcgccaccaa tcagccagat
      661 tgatttttcc accagtgcat ctgcgtcacg cagcagttca tgtgcttcgg caacatcgtt
      721 gagttgctgg cgtaaagctg ctacctgttc acgacgaacc tctggcgtgg cgtctgattt
      781 caacgcagtc agtaattccg tcgggatttt atcggcaaat tgatcgagca gacgcagcac
      841 gcggacacgg tgttgatcga ccgtcaggcg gaaaccaagg ccaaattcgg cgttatcttc
      901 aaacagcgag ttcgcccatg ccggtccacg accgttggca tcggtggtat atggtgttga
      961 aggcaggtta ccgccgtata ttgaagaaca gccagtggca ttagcgatca acatccggtc
     1021 gccatagagc tgagtcagca atttaatata cggcgtctcg ccacaaccgg agcaagcacc
     1081 ggaatattca aacagcggcg tgattagctg cgatgtacga atatcaatac gttccagttt
     1141 gctacgatcg atttctggca ggttgaggaa gaaatcgtaa ttgattttct cttcttcgac
     1201 atgttccagg cgcgacatca tattgatggc tttaatctct ggattctgac ggtctttcgc
     1261 cgggcaaact tcgacgcaca ggttacaacc ggtgcaatct tccggagcca cctgcaagac
     1321 atatttctgc ccgcgcatat cacgcgattt cacatccagc gaatgcaggc tggcaggggc
     1381 gttttccatc gcttcaggcg gtaccacttt tgcgcgaata gctgagtgtg ggcaagcggc
     1441 gacacagtgg ttacattggg tacagagttc ctctttccag atggggatct cttcggcgat
     1501 attgcgtttt tcccagcgcg tggtgcccat cggccaggtg ccgtctggcg gcagcgccga
     1561 aacggggagg gcgtcgccaa gcccggcgag catcgcagcg gttacggttt tcacgaaatc
     1621 aggagcggca tcggaaacca ccggcggtcg attggcgcta tgcggattaa ctggctgcag
     1681 tggaacttct tctacggatt cacgtgccag cgccagagcc tgccagttgc gttccaccag
     1741 atcctggcct ttgctactgt aacttttggc aatcgcaccc tgcaattctg cgagggcact
     1801 atcgccaggc agaatctgcg tcagatggaa aaaagccatc tgcatgacgg tattaatacg
     1861 ggccgccagg ccacattcgc gggcgatttt cgccgcgtta atcacataga agcgcgcttt
     1921 tttctggttt agcacggcct gaacttcctg cggcaagcgc gaccacactt cagctgcgct
     1981 gtacggcgtg ttgagcagga aaatgccgcc aggttttaaa cgctcagcca tctgatattt
     2041 atcgataaac tgcaactggt ggcagccaac aaaatcagcc tgggaaatga gataagcgga
     2101 acgaatcggc tgttcgctca cgcgaaggtg agaaaccgtc aggccgcccg cctttttgga
     2161 gtcgtaaaca aaatagccct gtgcgtacca cggcgtggaa tttccgataa tcttgatatt
     2221 gtttttggtc gcggaaacgc tgccatcact accaaggcca taaaacaagg cttccagttt
     2281 cgccgagttt ggtagggtgt tttccggcaa cggcagtgac agattggtta catcatcgta
     2341 aataccaacc gtaaagcgcg ctttcggttt agccgcgttg agctcggcaa ataccgccag
     2401 tacacagtcc gggccaaatt ctttggatga aagaccatag cgcccaccaa tgacacgggg
     2461 cagggtttcg cgctcgccat tattaaaggc ttctgccagt gcagtcatca catccagata
     2521 gagcggttct gcctgggcac cgggttcttt ggttctgtcc agtaccgcca cgttgcgcac
     2581 ggatcccggc agagcttgca gcaagtgttt ggcggagaag gggcggtaca ggcgaacttt
     2641 cagcacgccg actttttcgc cacgggtcag caattcatca accacttctt cactggtgcc
     2701 aatggcagag cccatcagga taatcacccg ttccgcttgc ggatgcccgt aatactcaaa
     2761 cggctgatac tgacgacctg tcgcggcagc gaaatcattc atcgcctgtt caacatggtc
     2821 atagaccgcg ttgtaccatg ggttggtggc ttcgcgagac tggaaataag tgtcaggatt
     2881 ggcggacgta ccgcggatca ccggatgttc cgggttgagt gcccgggcgc gatgagcatc
     2941 aatttctgcc tgcggcatca gttcaagaat cgtgtcatcg gccagcggga caattttatt
     3001 gatttcgtga gatgtgcgga aaccatcaaa gaaatgaata aatggcacgc gacttttcag
     3061 cgtcgcaatg tgcgaaatga gagcaaagtc ttgcgcttcc tggacgtttg ctgcacacaa
     3121 catcgcgcaa cccgtttggc gcactgccat aacgtcggaa tgatcgccaa aaatagagag
     3181 tgcatgtgtg gcaacggtac gtgccgcaac atgcaggaca aacggtgtga gttcgcctgc
     3241 cagtttgtac agcgttggga tcatcagcag caaaccctgc gatgacgtaa acgatgttga
     3301 gagggcacca gtttgcaaag caccatgcac ggtagcgata gcacccgctt ccgactgcat
     3361 ttcaaccacg cgtggtgtgt ctccccaaac gtttttcaag ccgtttccgg cccatgcatc
     3421 agcctgttct gccatcgtgg aactgggggt aatagggtag atggcgataa cttcactggt
     3481 gcgaaacgcc accgaagcaa ccgcgccatt accgtcaata gtaatcatat gacaccctta
     3541 cattgcgcaa atgaggggcg cacgaaattg ctgcgcgccc agtagtaatc gttcaatttt
     3601 agcaaatggc tttcttctgc attttcgctt ttgtgtcccc cacatcagcg taatgaatgt
     3661 tttgatcaaa cagagggcaa aaaaatagcc ataaaaaagt aaaaatgcgg ggcaacgcgc
     3721 aggacagctc tcgacgccgc cctctgtgct gcctattatt tatggcggtg tcgtttggct
     3781 tgaagatcag aggaagaaaa gatgcgagca gcgttttggg tagggtgtgc cgctttattg
     3841 ttgtcggcgt gtagtcgtga acctgttcag caggcgactg cggcgcacgt agcgccaggt
     3901 ttaaaagcgt cgatgtccag tagtggagaa gcaaattgtg caatgatcgg cggttcgctt
     3961 tctgttgccc gtcaactgga tggtacggcg attgggatgt gtgcattacc caacggcaaa
     4021 cgctgtagcg aacagtcact tgccgccggg agctgtggca gctattaatt cattaaatcc
     4081 gccagcttat aagttaatgt ctgtttcgcg gtcgccagcg ttaactggtt cgcggtcaga
     4141 tccacttgtg caccttcttt cagcatttcg ctaatggtgt tatcgagttc attaagctgc
     4201 gggttagcgc acatcatacg ggtcattgcc agccctttgg cggtcagttc accattagac
     4261 agtttgcctt caccgctaaa gcggttacac atgctgccgg aaatcatcat cttttcacca
     4321 aagctgattt ctggcggatt tttatcgctg gtcacgggct taccgtttac gctttccagc
     4381 acaaagcgat gatgctgtag ctgttctggc gttacagcaa ttttgtcact acttacacat
     4441 cccgccatca gcaggcttag cgcaacaagc gcggctactt tcttcattgt ggttctcaat
     4501 tactgtttct gattcaggac tattttaaga atagaggatg aaaggtcttt ggggattatc
     4561 tgaataagct cccctggaat gcaggggagc ggcaagatta aaccagttcg ttcgggcagg
     4621 tttcgccttt ttccagattg cttaagtttt gcagcgtagt ctgagaaata ctggtcagag
     4681 cttctgctgt caggaatgcc tggtgcccgg taaacagcac gttgtggcag gcagacaagc
     4741 gacggaatac gtcatcctgg atcacgtcgt tggatttatc ttcaaagaac agatcgcgtt
     4801 cgttctcata cacgtccata cccaacgaac caattttctg atttttcagc gcttcaattg
     4861 ccgcctgaga atcaatcaat gcaccgcgac tggtattgac gatcatcacg ccatttttca
     4921 tctgatcgaa ggcggcttcg ttcaacagat ggtagttttc cggtgtcagc gggcagtgca
     4981 gagagataac gtctgattca gagaacaggg ttggcagatc gacatactcc acaccgagtt
     5041 ccagcgccgc cgcacttgga tacggatcga acgccagcag acgcatacca aaacctttca
     5101 gaatgcgcag catcgccaca ccgattttac cggtaccgat cacgcctgcc gttttgccat
     5161 acatagtaaa gccggtcaga ccttccagag agaagttagc gtcacgggta cgctgatacg
     5221 cgcggtgaat acggcggttc agcgtcatca tcataccgat ggcgtgttca gctacggcct
     5281 ctggatcata ggctggaaca cggactactt tgagccccag ttcttttgcc gcgtcaaggt
     5341 cgacgttatt gaaaccggca cagcgcaggg cgatatattt aacgccgtgc tttttcagct
     5401 cttccagcac cgggcggctg ccgtcatcgt ttacgaaaat acataccgct tcgcagccat
     5461 tggcagtttt agcggttttt tccgtcagca gaaagtcaaa aaattccagc tcaaagccaa
     5521 aggactcgtt cacctgttgc aggtacttct tgtcgtactg ttttgtgcta taaacggcga
     5581 gtttcataag actttctcca gtgatgttga atcacattta agctactaaa aatattttac
     5641 aaaatttcaa atataattga aagttatggc gatattgaaa aattcatcaa caactatgct
     5701 tagtgtaggt gcaaccttca actgaacggt taaacatgcc acaatacccg tattgaatgc
     5761 ttaatttttc gctaaatcag gatattaact acccatgctg ggtaaatata aagccgttct
     5821 cgcgctgtta ttactgatta ttcttgtgcc gttgacgctg ctgatgacgc tcgggctgtg
     5881 ggttcccacg ctggcgggca tctggctacc gctcgggaca cgtattgcat tagatgaaag
     5941 cccacgcatt acgcgtaaag gtttaatcat ccccgatctc cgttatctgg tgggagattg
     6001 tcagcttgcg catatcacca acgccagcct ttcacatccc agccgctggt tattgaacgt
     6061 cggcacggta gaacttgatt ctgcttgtct ggcgaaattg ccgcagacgg agcaatcgcc
     6121 agccgctccc aaaaccctcg cgcagtggca ggccatgttg cctaacacct ggatcaatat
     6181 cgataaactg attttttctc cctggcagga atggcaggga aaactctctc tcgcattaac
     6241 ctccgatatc cagcaactgc gttatcaggg cgaaaaagtt aaatttcaag gccagcttaa
     6301 agggcaacaa cttacagtca gcgaactgga tgtcgtcgcg tttgaaaatc agccgccggt
     6361 aaaactggtg ggggaattta ctatgccgct cgtgccggat ggacttcctg taagtggtca
     6421 tgctactgcg acgttaaact taccgcagga gccttcactg gtggatgccg agctggactg
     6481 gcaggaaaat agcgggcaat tgattgtgct ggcacgggat aacggcgatc cgttgctcga
     6541 tttgccgtgg caaattactc gtcaacaatt gaccgtaagc gatggtcgct ggagctggcc
     6601 gtatgcaggt tttcctttga gtggccgact gggtgtcaaa gtcgacaact ggcaggcagg
     6661 gctcgagaac gctctggtca gcgggcgact gagtgtgctg acccaggggc aagcgggtaa
     6721 aggcaacgcg gtgttaaatt ttggcccagg aaaattaagc atggataaca gccagctgcc
     6781 tctgcagctg accggtgaag cgaaacaggc ggacctcatt ttatatgccc gtttacctgc
     6841 gcagctaagt ggaagtctgt ctgacccaac gctgaccttt gagcctggcg cgttacttcg
     6901 ttcaaaggga agagtcattg attcgctgga catcgatgaa atccgctggc ctttagcggg
     6961 tgtaaaagtc acccaacgtg gtgttgacgg acgtttgcag gccattttgc aggcgcatga
     7021 aaatgaactg ggcgatttcg tgctgcatat ggatgggctg gcgaatgatt ttctccctga
     7081 cgctggccgc tggcagtggc gctactgggg aaaagggagt tttacaccga tgaatgccac
     7141 ctgggatgtc gcaggaaaag gtgagtggca tgacagcacg attacgctga ccgatctctc
     7201 caccggtttc gaccagttac aatacggtac gatgacggta gaaaagccgc gattaattct
     7261 cgacaagccc gtcgtctggg gacgtgacgc acagcatccc tccttcagcg gcgctctgtc
     7321 actggacgcc gggcaaacgc tgttcactgg cggcagtgtg ttaccgccat caaccttaaa
     7381 atttagtgtc gatgggcgcg atccgacgta tttcctcttt aaaggcgatt tacatgctgg
     7441 tgagattggc ccggttcggg taaatggtcg ctgggacggt attcgtctgc gcggtaacgc
     7501 ctggtggcct aaacaatcac tgactgtatt ccagccgttg gcgccacccg actggaagat
     7561 gaacttacgc gatggtgaac tgtatgctca ggtcgcattt tcagcagcgc ccgaacaagg
     7621 attccgcgcg ggggggcacg gcgtattgaa aggcggtagt gcctggatgc cagataatca
     7681 ggttaacggt gtcgattttg tcctgccttt ccgttttgcc gatggagcct ggcatctggg
     7741 gactcgcggc cccgttacgt tgcgaattgc cgaagtgatt aatctggtga cagcgaaaaa
     7801 tattacggct gatttgcaag ggcgttatcc gtggactgaa gaagaaccat tgctgttgac
     7861 tgatgttagt gtcgatgtgt taggcggtaa cgtgctgatg aaacaattac gtatgccgca
     7921 acatgacccg gcactgttgc gactgaataa tctctcatcc agcgaactgg ttagcgccgt
     7981 taatccgaaa caattcgcca tgtccggggc atttagtggt gcactgccgt tatggttaaa
     8041 caatgaaaaa tggatagtga aagacggctg gctggcaaat agcggaccaa tgaccttgcg
     8101 gctggataaa gacaccgcag atgcggtggt gaaagacaat atgaccgcgg gttcagcaat
     8161 taactggttg cgctatatgg aaattagccg ttcatcgaca aaaattaatt tagataatct
     8221 cggtttatta accatgcagg ccaatattac aggtaccagt cgcgttgatg gtaaaagcgg
     8281 tacggtaaat cttaattacc atcatgaaga gaatattttt acgctgtggc gcagtttacg
     8341 ctttggcgat aatctccagg catggctgga gcagaacgcg cgtctgccgg gaaatgactg
     8401 tccgcaagga aaagagtgtg aggataaaca atgaaaattt tactggctgc gttgacgtca
     8461 tcttttatgc tggttggctg tacgcctcgt attgaagtcg ctgcacctaa ggaaccgatc
     8521 actatcaata tgaacgttaa aattgagcat gagatcatca tcaaggcaga caaagatgtc
     8581 gaagagctgc ttgagacccg tagcgatctt ttttgaggtg atgatgaaaa gaacattact
     8641 tctttgtgca tttcttgttg gcttggtaag cagcaatgta atggcattga ctctggatga
     8701 agccagaact caggggcggg taggcgaaac attttatggt tatctggttg cgctgaaaac
     8761 agatgctgaa actgagaaat tagtaaccga cattaatgcc gaacgtaaag cgagctatca
     8821 acaactggca aagcaaaata atgtgtcggt agatgatatc gcgaaactcg ccgggcaaaa
     8881 gctcgtagaa cgggccaaac ccggggaata tgtacaaggg ataaacggta aatgggtgcg
     8941 aaaattttaa tagtaaagcc gcaatatcgg gataacgcta caggagaagg cgattaatct
     9001 attttccgga aacaagatga ttattcaaaa acgcctgtca aattgccttt cgccctgaaa
     9061 aatgcatagt caaatatctg ttttaactaa ttcgcgttgc tgtacatgca acgcgaatta
     9121 gttagcggaa tttacgccga ttctcgcctg gcgtcatccc aaagcgttgt ttaaataccg
     9181 ttgaaaaatg actctggtcg gaaaatcccc aatgaaagcc aatgcctgcc agtttttcat
     9241 catctgcggc atggcgaatc gcatctgcac aaaaatcgag acgacggtta cgaatatatt
     9301 gcgcgaccac caaaccttta tcggcaaaca ttcggtacaa actacgtact gacatacctg
     9361 tatctccgac tattcttcac ggctgcatta accaccgcca tacattagtg ggaattaacg
     9421 cggtagtgat ggacggtgtg gtgattggtg agaacagtat tgttggtgtt tccgcatttg
     9481 tgaaagccaa agcagaaatg ccgactaatt acttgattgt cagcagcccg gcgaaagcga
     9541 ttcgtgaact cagcgagcag gagctggcat tgagaaagca gggcatcagg tgctggtgac
     9601 tcgttgtaag cagacgttgc atcaagtcga gccattgcgg gaagttgaac ctggcaggaa
     9661 acgcctggtg tttgatgaga atctgcgacc gaa
//
LOCUS       DAJOTB010000168         9680 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJOTB010000168 DAJOTB010000000
VERSION     DAJOTB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.168"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            430..2061
                     /gene="oppA"
                     /locus_tag="OHN28_004128"
     CDS             430..2061
                     /gene="oppA"
                     /locus_tag="OHN28_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCQ2299421.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2147..3067
                     /gene="oppB"
                     /locus_tag="OHN28_004129"
     CDS             2147..3067
                     /gene="oppB"
                     /locus_tag="OHN28_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCQ2299422.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3082..3990
                     /gene="oppC"
                     /locus_tag="OHN28_004130"
     CDS             3082..3990
                     /gene="oppC"
                     /locus_tag="OHN28_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCQ2299423.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4002..5015
                     /gene="oppD"
                     /locus_tag="OHN28_004131"
     CDS             4002..5015
                     /gene="oppD"
                     /locus_tag="OHN28_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCQ2299424.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5012..6016
                     /gene="oppF"
                     /locus_tag="OHN28_004132"
     CDS             5012..6016
                     /gene="oppF"
                     /locus_tag="OHN28_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCQ2299425.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6069..6398)
                     /locus_tag="OHN28_004133"
     CDS             complement(6069..6398)
                     /locus_tag="OHN28_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCQ2299426.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6433..7893)
                     /gene="cls"
                     /locus_tag="OHN28_004134"
     CDS             complement(6433..7893)
                     /gene="cls"
                     /locus_tag="OHN28_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCQ2299427.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8036..8209
                     /locus_tag="OHN28_004135"
     CDS             8036..8209
                     /locus_tag="OHN28_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCQ2299428.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8264..9517)
                     /gene="kch"
                     /locus_tag="OHN28_004136"
     CDS             complement(8264..9517)
                     /gene="kch"
                     /locus_tag="OHN28_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCQ2299429.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2527 a         2262 c         2417 g         2474 t
ORIGIN      
        1 aagagagaca tgcttaacta acctctattt gattgaattg aaagatgttt gttaaggcat
       61 ggatgcaagc tatagattct ggtacggtca ataaaagaga attgcttaac aattttgcaa
      121 aatgtattgg cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca ggcgattctt
      181 tattggtaat gagaataatt aacaattaaa gaccgtcgcg aaagaataat gtgtctcgac
      241 aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga ataaataaag
      301 tcggtgatag taatacgtaa cgataaagta acctgacagc agaaagtctc cgagcctgtg
      361 cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa tgagggagtc
      421 caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt tttggctgcg
      481 cttatggcag ggaatgtcgc gctggcagct gatgtacccg caggtgtcac actggcggaa
      541 aaacaaacgc tggtacgtaa caatggttca gaagttcagt cattagatcc gcacaaaatt
      601 gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact ggttagcgat
      661 cttgacggcc atccggcacc tggcgtcgct gaatcctggg ataataaaga cgcgaaagtc
      721 tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt cacagcacaa
      781 gactttgtgt atagctggca acgttctgtt gatccaaaca ctgcttctcc gtatgccagt
      841 tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa aaaaccgatt
      901 accgatctcg gcgtgaaagc tattgatgac cacacattag aagtcacctt aagtgaacct
      961 gttccgtact tctataaatt acttgttcat ccatcaactt caccggtgcc aaaagccgct
     1021 atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa cggtgcctat
     1081 accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc gacctactgg
     1141 aacaacgcga aaaccgttat taaccaggta acctatttgc ctattgcttc tgaagttacc
     1201 gatgtcaacc gctaccgtag tggtgaaatc gacatgactt ataacaacat gccgatcgaa
     1261 ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc atacctgtgc
     1321 acttactatt acgaaattaa caaccagaaa ccgccattta atgacgtgcg tgtgcgtacc
     1381 gcactgaaat tgggtatgga ccgcgatatc attgttaata aagtgaaagc gcagggcgac
     1441 atgcccgcct atggttacac tccaccgtat actgatggcg caaaattgac tcagccggaa
     1501 tggtttggct ggagccagga aaaacgtaac gaagaagcga aaaaactgct ggctgaagcg
     1561 ggttataccg cagacaaacc gttgaccatc aacctgttgt ataacacctc cgatctgcat
     1621 aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt aaacgtcaaa
     1681 ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggcac ttttgatgtg
     1741 gcccgtgcag gctggtgtgc agactacaac gaaccaactt ccttcctgaa caccatgctg
     1801 tcgaacagct caatgaatac cgcgcattat aagagcccgg cctttgacag cattatggcg
     1861 gaaacgctga aagtgactga cgaggcgcag cgtgcagctc tgtacactaa agcagaacaa
     1921 cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc gcgtttggtg
     1981 aaaccgtggg ttggtggcta taccggcaaa gatccgctgg ataataccta cacccggaat
     2041 atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc tccacggtgt
     2101 ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt taaaatttat
     2161 tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta tttcgttctt
     2221 tatgatgcgc ctcgcgccgg gcagtccttt taccggcgaa cgtactttac caccggaagt
     2281 gatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac agtatttcag
     2341 ctacctgaaa caactggcgc atggcgattt cggtccatcg tttaaatata aagattattc
     2401 ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc aaactgggag ctgcagcatt
     2461 ttttcttgcg gtaatactgg gtgttagtgc tggcgttatt gccgcattaa agcaaaacac
     2521 caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc ccagttttgt
     2581 ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc cgggcggtgg
     2641 ctggaatggt ggagcgctta aattcatgat attgccaatg gtggcgttat cactcgccta
     2701 tatcgccagt attgcgcgta ttacccgtgg ctctatgatt gaggttttac actccaactt
     2761 tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac gccacgcatt
     2821 aaaacctgct ctgttacccg tgctctccta tatggggccc gcatttgtcg gcattattac
     2881 cggttcgatg gtcatcgaaa ccatttatgg tttaccgggg attgggcaat tgttcgttaa
     2941 tggcgcattg aaccgtgact attccttagt gttaagtctg accattctgg ttggcgcgtt
     3001 aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc cgaaaatccg
     3061 ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac gctggaaaat
     3121 ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg tcgacgtttt
     3181 atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc gttatttgta
     3241 atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc gatgatgtcc
     3301 agcgccccgg atatggagtc cggtcactac tttggtactg actcatccgg tcgcgacctg
     3361 cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc tgcggcactg
     3421 gtggcagtgg tcgtggggac actttatggt tcgctttccg gttatctggg cggtaaagtg
     3481 gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat gttcttcgtc
     3541 attttgctgg tgaccttttt cggtcaaaac atcctgctga ttttcgtggc gattggcatg
     3601 gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct gaagcgcaaa
     3661 gagtttattg aggcggcaca agttggcggt gtatcgacgc cgggcattgt tattcgccac
     3721 attgtgccga acgtactcgg tgtggtggtg gtctatgcgt cgcttctggt gcccagcatg
     3781 atcctctttg aatctttcct tagcttcctg ggattaggta cacaagagcc gttaagcagc
     3841 tggggtgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg gttactgttg
     3901 ttcccagcgg gattcctcgt ggtaacgctg ttttgtttca actttatcgg cgatggcttg
     3961 cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta attgaaactg
     4021 caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa gatttgcgtg
     4081 tcacctttag taccccggat ggcgacgtca cggcggtcaa tgatttgaat ttttcgctac
     4141 gtgccggaga aacgctgggc atcgtaggtg agtctggttc gggcaaatcg caaaccgcat
     4201 tcgcgttgat gggcctgtta gctgccaacg gacgtattgg cggatcggca accttcaatg
     4261 ggcgtgaaat cctcaatttg ccagagcatg aactcaataa actgcgagct gaacaaatct
     4321 caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc ggtgagcagt
     4381 tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt gaagaatcgg
     4441 tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa atgtacccgc
     4501 acgaattttc tggcggcatg cgtcagcgag tcatgattgc gatggcattg ctatgtcgac
     4561 ctaagctgct gattgcggat gaacccacta ctgcgctgga cgtcaccgta caggcgcaga
     4621 tcatgacgct attgaatgaa ctgaagcggg aatttaatac cgccatcatt atgattaccc
     4681 acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac gccgggcgca
     4741 cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgtacatcct tattctatcg
     4801 gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga aacaatgttg actattccgg
     4861 gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca cgttgtccgc
     4921 atgcgatgga tatttgtagt agcgcaccgc cgctggaaga gtttacgcct ggccgtctgc
     4981 gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag gaagaaaagt
     5041 cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca aacagtggtt
     5101 ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgta actcttcgcc tgtatgaagg
     5161 ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg ctcgcgccat
     5221 cattggcctg gttaaagcga ccgacggtca tgttgcctgg ttaggtaaag agttgctggg
     5281 catgaagccc gatgaatggc gtgccgtacg cagtgatatt cagatgattt tccaggatcc
     5341 gttggcatcg ctgaacccgc gtatgaccat cggcgagatc atcgctgaac cactgcgtac
     5401 ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga tgatgctgaa
     5461 agtcggtttg ttgcctaacc tgattaaccg ctatccgcat gagttctccg gtgggcagtg
     5521 ccagcgtatc gggattgctc gcgctcttat tcttgaaccg aagctgatta tctgtgatga
     5581 gccggtgtcg gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc tccagcagtt
     5641 gcaacgtgag atgggattgt cattaatttt tatcgctcac gacctggccg tggtaaaaca
     5701 catttccgat cgtgtgctgg tgatgtatct cggccatgcg gtagaactgg ggacctatga
     5761 tgaggtctac cacaatccgc tacatcctta caccagggca ttgatgtcgg cagtccccat
     5821 acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg aattaccgtc
     5881 gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt tgtccgattg caggtccgga
     5941 gtgcgccaaa acacgtcctg tgctggaagg gagtttcaga cacgccgttt cttgcctgaa
     6001 agtcgatccg ctttaaaagc aataagggct gacagttgtc agcccttatt cacgccaaaa
     6061 gcgattattt attcccgcca gatgatatgg caaagtttgt gatctttttc gcggcataac
     6121 agaatgcgcg cgaaaacatc gttgatttca ccatcttcac tgtccgccag accaatcacc
     6181 acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat gttcctgcca gtcttccgcc
     6241 ggatcgaata actccgcgcc gccgcgctct tcaaactgaa gattgaacag cagaacatca
     6301 gctggatcga gattgtcggc agccagttcg agaaaaatat cgtaggcctg ctcaagcgtt
     6361 tcatcttcag tcaggcgatt gtttagatcc atatccatag tcactacctg tttaacctct
     6421 gttggcgacg ttttacagca acgggctgaa gaagtaaaac agtcgctcgg cgacacgttg
     6481 ccataatgga cgttttagcc ataaacgggc atcgagcaga cgagaacgcg aaatataatc
     6541 gtcctgaacg gcggcgaggt ctgcaccaaa acctttatcg tcgattgcca gggtaatctc
     6601 gaaatttagc cacagactac gcatatcaag gttaacggtg ccaaccagac ttagttctcc
     6661 atcgaccagc acgctcttgg tatgcagtaa cccgccttca aactgataaa ttttaacccc
     6721 agcagccagc agttctgtaa agaatgcgcg actggcccag ccgaccagca tcgagtcatt
     6781 ttttcgcgga aggataatac tgacatccac cccgcgctgc gccgccgtgc aaatcgcatg
     6841 aagtaaatca tcgcttggca caaaataggg cgttgtcatg atcaaatatt cacgagccga
     6901 ataagcagca gtcaataatg cctggtgaat gagatcttcc ggaaagccgg ggccagaagc
     6961 aattgtgtga atggtgtgac cactggcctg ctcaaacggc ataatattga catctggtgg
     7021 cggcggcaga atacgtttcc cggtttcaat ctcccagtcg caggaataaa taatccccat
     7081 cgcggtggcg atggggcctt ccatacgcgc catcagatca atccattgcc ctacacccgc
     7141 atcttgtttg aagtagcgag ggtcgaccat attcatgctg ccggtgtacg cgatgtaatt
     7201 atcgatcatg atcatcttgc gatgttggcg caggtccata cggcgtaaaa acacacgcat
     7261 cagattgacc tttaaggctt cgacaacttc aataccggca ttacgcatta gctcgggcca
     7321 cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc atcaatcggc aatgaatacc
     7381 gcgccgtgca gcggccatta atgattcagc cacctggtcc gccatgccgc cgggctgcca
     7441 gatataaaac accatctcaa tattatggcg cgcgagctgg atgtcgcgga ttaacgcctg
     7501 catcacatca tctgactcgg tcatcagttg tagctgattc cctttgaccc cagcgatccc
     7561 ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca ctgctatttt cttcggcgaa
     7621 gatatgctta caggctttaa ggtcgttaag ccattttgcg gtggaaggcc acatcgctct
     7681 ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca acggcaagat aggcaataat
     7741 tccgactaac ggcagaatgt aaataatcaa cagccaggcc atcgcggagg gaactgcgcg
     7801 tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc aaccagtatc ccagaatggc
     7861 caaccaactc accaacgtat aaacggttgt catagattag aaatcctttt gaaagcgcat
     7921 tgttatgagt ttacgcatat ggattcatct ggcaaataaa aacgagggaa aagcactggt
     7981 cagcgtcagg ggcgggggta taatgaccat tctgttgttg catagagtag ttaacatgaa
     8041 gcggagtaga acggaagtgg ggcgctggcg catgcagcgt caggctagcc gacgtaaatc
     8101 gcgttggctt gaggggcagt cgcgccgaaa tatgcgtatc cacagcatca ggaagtgcat
     8161 tctaaacaaa cagcgtaact cgttattgtt tgcgatctac aatatctaaa tgtactgggc
     8221 accattgcgg tgcccaataa gtttactctt ccgtctttaa actctatttt tgcgccgatt
     8281 ctttactgtc agctttcgtt tcttgttcat cgttatcgct gaaaatgcca tgaccggagt
     8341 tcaacaacat tgaaacgagc atatcattat taatctcttc accatttaaa actcgcgcca
     8401 gaatttcgct gccaaacagt tgtggtgaga gaatgatatc cggatgtacc atcttaatct
     8461 tattcaggtt tttgctatca ctgacggcaa gaactgtttt gacatcactg ctcatatctt
     8521 ttgccgagag tacaacaaac gcgttatctg catcgttatc actcagcgcc agaatggccc
     8581 ggcaacgatc gattcccgct ttctttaaca ctgaactgtc attactgtca ccggggataa
     8641 catcggcgtt atcgcctaag cgttgctcaa gttgcttgat atcatcttca ggcaagttgc
     8701 tgataaccgt tacgttttgt ccgcgttgat tcagttgcag aatcgtattg atggcgagaa
     8761 tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat tgtatgattg tttcctttta
     8821 caagtttgtt gaatcccccg cggataagcg gaccaaaaat tgaggtcata gatgtggcaa
     8881 aaacggtaat gccggaaata atgaccgaaa tagtgaacaa tcgtgctgat tcagaaacag
     8941 ggacaatatc gccatagccg acggttgaca tggtttctat cgaaaaatag aacgcggtca
     9001 tcaaactttc tattcgcgga ttaaaacctt cgcttaaata aagcgcaccg taagtggagt
     9061 aaaacagtaa cgtcgtgaaa ctaataaatg cgaaaattgt cccggctgct gcgctactgt
     9121 gggagaagtc tttgctcagt atcagcaaaa acaccagcgt aaaaatgcaa aatccaatac
     9181 taaatttcag ccagggataa aaatgcagag tgtaaattag cgctatcaac aacaaaatga
     9241 tactgattgc ccatgcgagc tttgcccgga acagtaagcc aatggagttc aggacaagaa
     9301 atacgccgag cataaatagt ggggcgtttg ccagcaagga gagatccagc tctgaaaatg
     9361 ctttgatatg gaaaatatca agcaaattga ccgacatacc atagattgtt ttaaaaataa
     9421 gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg ccgtagggtc acccataact
     9481 ttgttgctgt ttgtttgaat gtagtccagt gactcactga tacctctgta accacaattt
     9541 tgtttaatta tctcaacgtg cacattttct gaacatacat gcagctcgtg aatgtgttaa
     9601 acaatagtag atgactttat ccgcagtaat caaaggtttt gagtgagcaa ctctgcgtca
     9661 ggaaagggca ttttcctgaa
//
LOCUS       DAJOTB010000169         9646 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJOTB010000169 DAJOTB010000000
VERSION     DAJOTB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.169"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            39..1013
                     /gene="cysB"
                     /locus_tag="OHN28_004137"
     CDS             39..1013
                     /gene="cysB"
                     /locus_tag="OHN28_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCQ2299430.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1308..1472
                     /locus_tag="OHN28_004138"
     CDS             1308..1472
                     /locus_tag="OHN28_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCQ2299431.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
     gene            1475..1642
                     /locus_tag="OHN28_004139"
     CDS             1475..1642
                     /locus_tag="OHN28_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299432.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1756..1851
                     /locus_tag="OHN28_004140"
     CDS             1756..1851
                     /locus_tag="OHN28_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299433.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2001..4676
                     /gene="acnA"
                     /locus_tag="OHN28_004141"
     CDS             2001..4676
                     /gene="acnA"
                     /locus_tag="OHN28_004141"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCQ2299434.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSFKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4740..5330)
                     /gene="ribA"
                     /locus_tag="OHN28_004142"
     CDS             complement(4740..5330)
                     /gene="ribA"
                     /locus_tag="OHN28_004142"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCQ2299435.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5500..6264
                     /gene="pgpB"
                     /locus_tag="OHN28_004143"
     CDS             5500..6264
                     /gene="pgpB"
                     /locus_tag="OHN28_004143"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCQ2299436.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6413..6721
                     /gene="lapA"
                     /locus_tag="OHN28_004144"
     CDS             6413..6721
                     /gene="lapA"
                     /locus_tag="OHN28_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCQ2299437.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6728..7897
                     /gene="lapB"
                     /locus_tag="OHN28_004145"
     CDS             6728..7897
                     /gene="lapB"
                     /locus_tag="OHN28_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCQ2299438.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8089..8826
                     /gene="pyrF"
                     /locus_tag="OHN28_004146"
     CDS             8089..8826
                     /gene="pyrF"
                     /locus_tag="OHN28_004146"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCQ2299439.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRDFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8826..9152
                     /gene="yciH"
                     /locus_tag="OHN28_004147"
     CDS             8826..9152
                     /gene="yciH"
                     /locus_tag="OHN28_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCQ2299440.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(9278..9496)
                     /gene="osmB"
                     /locus_tag="OHN28_004148"
     CDS             complement(9278..9496)
                     /gene="osmB"
                     /locus_tag="OHN28_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCQ2299441.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2391 a         2256 c         2508 g         2491 t
ORIGIN      
        1 aaacgatgtt ctgatggcgt ctaagtggat ggtttaacat gaaattacaa caacttcgct
       61 atattgttga ggtggtcaat cataacctga atgtctcatc aacggcggaa ggactttaca
      121 catcacaacc cgggatcagt aaacaagtca gaatgctgga agacgagctg ggcattcaaa
      181 ttttttcccg aagcggcaag cacctgacgc aggtaacgcc agcaggacaa gaaataattc
      241 gtatcgctcg tgaagttctg tcgaaagtcg atgccataaa atcggtcgcc ggagagcaca
      301 cctggccgga taaaggctcg ctgtatatcg ccaccacgca tactcaggca cgctatgcgt
      361 taccaaacgt tatcaaaggc tttattgagc gttatcctcg cgtttctttg catatgcacc
      421 agggctcgcc gacacaaatt gctgatgccg tctctaaagg caatgccgat ttcgctatcg
      481 ccacggaagc gctgcatctg tatgaagatt tagtgatgct accgtgctac cactggaatc
      541 gggctatagt agtcactccg gatcacccgc tggcaggtaa aaaagccatt accattgaag
      601 aactggcgca atatccgttg gtgacatata ccttcggctt taccggacgt tcagaactgg
      661 atactgcctt taaccgcgca gggttaacgc cgcgtatcgt cttcacggca acggatgctg
      721 acgtcattaa aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg
      781 tggatccggt cgccgatccc gaccttgtgc gtgttgatgc tcacgacatc ttcagccaca
      841 gtacaaccaa aattggtttt cgccgtagta cttttttgcg tagttatatg tatgatttca
      901 ttcagcgttt tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct
      961 ctaatgaaga aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg
     1021 cgttattttc ggcacctttt atgtagcgaa ggtgccggaa tatattctcc tttgttactt
     1081 atttgtatcc ggtgcacaat atttttatcc ctcatattta tagggtaaat tacctgtaaa
     1141 gtagtgtcta attacacacc tcgcaaactc cagttttcaa atatctattt caggtcaaaa
     1201 gattgaatta ttcactggag acgattcgta aattcgctgg atttttgact aaagtttctt
     1261 taggatttgt ctcatcgtat gattaattac accaatcgtt caacgtcttg ctgataagtg
     1321 atggcgatta tatgaggtta gcaatgcctt ctggaaatca ggaaccccgc cgcaatcctg
     1381 aattaaaacg taaagcctgg ctggcggttt ttcttggttc tgcacttttc tgggtggttg
     1441 tcgcactgct tatttggaaa ttgtggggat aaccatggtc ggtcaggagc aactggagtc
     1501 gtcaccatta tgtcagcata gtgacaatga accagaagca aaacgggaat gttccgtcgt
     1561 tattccagac gactggcaac taacatcgca gcaacaagcc tttatagaac tgtttgctga
     1621 agatgatcag ccgaaacaat aattatcatc attcttatta cccattttta atgaattaca
     1681 gggcttttaa tacaccgcag caataacagg ttgagttatc tcaacacaaa gtaataaccg
     1741 ttaagggtgt agcctatgat caacacaaat atgaaatatt ggtcctggat gggcgcattt
     1801 tctctgtcga tgctcttctg ggccgaactc ctctggatca ttactcactg atccctgatc
     1861 ccgctgtttg ctttgcctca aggtttctcc tcttttatca atttgggttg ttatcaaatc
     1921 gttacgcgat gtttgtgtta tctttaatat tcaccctgaa gagaatcagg gcttcgcaac
     1981 cctgtcatta aggaggagct atgtcgtcaa ccctacgaga agccagtaag gacacgttgc
     2041 aggccaaaga taaaacttac cactactaca gcttgccgct tgctgccaaa tcactgggcg
     2101 atatcacccg tctacccaag tcattcaaag ttttgctcga aaacctgctg cgttggcagg
     2161 atggtaaatc ggttaccgaa gaggatatcc acgcgctggc gggatggctg aaaaatgccc
     2221 atgctgaccg tgaaattgcc taccgcccgg caagagtgct gatgcaggac tttaccggcg
     2281 tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata
     2341 ctgcaaaggt taatccgctc tcaccggtcg acctggtcat tgaccactcg gtgaccgtcg
     2401 atcgttttgg tgatgatgag gcatttgaag aaaacgtacg cctggaaatg gagcgcaacc
     2461 acgaacgtta tgtgttcctg aaatggggaa agcaggcgtt cagtcggttt agcgtcgtgc
     2521 cgccaggcac aggcatttgc catcaggtta acctcgaata tctcggcaaa gcagtgtgga
     2581 gcgaattgca ggacggtgaa tggattgctt atccggatac actcgttggt actgactcgc
     2641 acaccaccat gatcaacggc cttggcgtgc tggggtgggg cgttggtggc atcgaagcag
     2701 aagccgcaat gttaggccag ccggtttcca tgcttatccc ggacgtagtg ggctttaaac
     2761 ttaccggaaa attacgtgaa ggtattaccg ccacagacct ggttctcact gttacccaaa
     2821 tgttgcgcaa acatggcgtg gtggggaaat tcgtcgaatt ttatggtgat gggctggatt
     2881 cactaccgct ggcggatcgc gccaccattg ccaatatgtc gccagaatat ggtgccacct
     2941 gtggcttctt cccaatcgat gctgtcaccc tcgattacat gcgtttaagc gggcgcagtg
     3001 aagatcaggt cgagttggtc gaaaaatatg ccaaagcgca gggcatgtgg cgtaatccgg
     3061 gcgatgaacc aatttttacc agtgtgttag aactggatat gaatgacgtt gaagcgagcc
     3121 tggcagggcc aaaacgcccg caggatcgcg ttgcactgcc cgatgtacca aaagcatttg
     3181 ctgccagtag cgaactggaa gtgaatgcca cgcataaaga tcgcctgccg gtcgattatg
     3241 ttatgaacgg acatcagtat cagttacctg atggcgctgt ggtcattgct gcgataacgt
     3301 cgtgcaccaa cacctctaac ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag
     3361 ccgtaactct gggcctcaag cggcaaccat gggtcaaagc gtcgctggca ccgggttcga
     3421 aagtcgtttc tgattatctg gcaaaagcga aactgacacc gtatctcgat gaactgggat
     3481 ttaaccttgt gggatacggt tgtaccacct gtattggtaa ctcgggaccg ttgcccgatc
     3541 ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg tgcagtgctg tccggcaacc
     3601 gtaactttga aggccgtatc catccgctgg ttaaaaccaa ctggctggcc tcgccgccgc
     3661 tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa tctggcttct gagcctatcg
     3721 gccatgatcg caaaggcgag ccggtatttc tgaaagatat ctggccatcg gcacaagaaa
     3781 ttgcccgtgc ggtagaccaa gtctccacag aaatgttccg caaagagtac gcagaagttt
     3841 ttgaaggcac agccgagtgg aaggaaatta acgtcacacg atccgatacc tacggttggc
     3901 aggaggattc aacctatatt cgcttatcgc ctttctttga tgaaatgcag gcaacaccag
     3961 cacctgtgga agatattcac ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca
     4021 ctgaccatat ctctccggcg ggcagtatta agcccgacag cccggcgggt cgatatctac
     4081 aaggtcgggg tgttgagcgg aaagacttta actcctatgg ttcgcggcgt ggtaaccatg
     4141 aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg
     4201 ttgaaggggg gatgacgcgc catttacctg acagcgatgt cgtctctatt tatgatgccg
     4261 caatgcgcta taagcaggag caaacgccgc tggcggtgat tgccgggaaa gagtatggat
     4321 caggctccag ccgtgactgg gcggcaaaag gtccgcgtct gcttggtatt cgtgttgtga
     4381 ttgccgaatc gtttgaacga attcaccgtt cgaatttaat aggcatgggc atcctgccgc
     4441 tggaatttcc acaaggtgtg acgcgtaaaa cgttagagct taccggggaa gagaagattg
     4501 atattggtga tctgcaaaat ctacaacccg gcgcgacggt tccggtgacg cttacgcgcg
     4561 cggatggtag ccaggaagtc gtaccctgcc attgtcgtat cgacaccgcg acggagttga
     4621 cctactacca gaacgacggc attttgcatt atgtcattcg taatatgttg aagtaacgag
     4681 tatttgcttg ccggttattt tgcttccggc aagcaaatta attacacaat gcaagagggt
     4741 tatttgttca gcaaatgacc cattttctcg gctttggtat cgagataatg ttcgttattg
     4801 gggttgcgac ctacaatcaa cggtacgcgt tcaataatat taatccctgc ttcggtcaga
     4861 atttcgactt ttttcgggtt attggttaac aagcggactt cattgacgcc aaggagtttg
     4921 aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta
     4981 gcctctacag tatcgtaacc ttgatcctgc agtgcgtaag cgcggatttt attcagcaga
     5041 ccaatgttac gaccttcctg acggtgatac agcaaaatac cacggccttc ctcggcaatt
     5101 tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc gcaagctgaa cagggcgtca
     5161 ccggtcagac attcggaatg gacgcgcgca agtaccgggg tatgcccgga aatatcgcca
     5221 tagactagcg cgacatgatc gtgtccggtt gccagttctt caaatcccac catcaggaaa
     5281 tcgccccatg gggttggcag tttggcttct gccacacgtt taagctgcat gaaattctcc
     5341 agataatgct ggttctgtat tggcttattt tgccataacg agaaggggtt cacctaatca
     5401 cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa tttttcctgg aactggcatt
     5461 ttcagttatg attgtgggac ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt
     5521 accgcagtgg gagctgcact attgcttgtc atgccagtag ccgtatggat ttctggctgg
     5581 cgttggcaac ctggagaaca aagttggtta ctaaaagcgg ctttttgggt tactgaaact
     5641 gtcacccagc cctggggcgt cattacacat ttgattttat tcggctggtt tctctggtgt
     5701 ctgcgttttc gcattaaggc tgccattatg ttatttgcca ttctggcggc agcaatcctt
     5761 atgggacaag gcgttaaatc ctggatcaaa gacaaagtcc aggaaccacg accttttgtt
     5821 atctggctgg aaaaaacaca tcatattccg gttgatgagt tctacacttt aaagcgagca
     5881 gaacgcggaa atctggtgaa agaacagttg gctgaagaga aaaatatccc acaatatttg
     5941 cgttcacact ggcagaaaga gacggggttt gcctttcctt ccggtcacac gatgtttgct
     6001 gccagttggg cactgctggc cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt
     6061 gctatcttgt tggtctgggc aacgggagtc atgggaagcc gcctgctgct cgggatgcat
     6121 tggccacgcg atctggttgt agctacgttg atttcgtggg cgctggtggc ggtggcaacg
     6181 tggcttgcgc aacgaatttg tgggccatta acaccacctg cggaagaaaa tcgcgaaata
     6241 gcgcaacgag aacaagaaag ttaacgctgg ttgattttcc gaatttagcc cttaaatcat
     6301 caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa atggtacttt aaagggctat
     6361 tgcggtaagt tgaccataat ttattcgctc taaccacata acgggaagta atgtgaaata
     6421 tttactcatt ttcttactgg tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca
     6481 gaacgatcaa caggtgacgt ttaattatct gttagcgcaa ggggagtacc gtatttccac
     6541 attgctggcg gtattgtttg ctgcggggtt tgctatcggt tggttgattt gcggcctgtt
     6601 ctggctgcga gttcgtgttt ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa
     6661 ccagctttca cccgcgactg acgtggctgt agcgccgcac tcgtctgcgg cgaaggaata
     6721 actttctatg ctggagttgt tgtttctgct tttgcctgta gccgctgcct atggctggta
     6781 tatgggccgc agaagtgcgc aacaaaacaa gcaagatgaa gccaaccgct tgtcgcgtga
     6841 ttacgtagcg ggggttaact tcctgcttag taatcaacag gataaagcgg tagacctgtt
     6901 tctcgatatg cttaaagagg atacaggtac cgttgaagcc caccttacgc tcggaaacct
     6961 gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc catcagaccc taatggaaag
     7021 cgcctcgctg acctatgaac agcggctttt ggcgattcaa caactggggc gtgattacat
     7081 ggctgccggg ttatacgacc gcgcggaaga catgttcaat cagctgacag atgaaactga
     7141 cttccgcatc ggcgcgctgc aacaactgct acaaatttac caggctacca gcgagtggca
     7201 gaaagcaatt gatgttgccg aacgcctggt gaagctgggt aaagataaac agcgcgtcga
     7261 aattgcccat ttctactgtg agttagctct gcagcatatg gccagcgacg atctcgatcg
     7321 tgccatgact ttgctaaaaa aaggtgcggc ggcagataaa aacagcgccc gcgtatccat
     7381 catgatggga cgcgtgttta tggcgaaagc agaatacgcc aaagccgtcg aaagtttgca
     7441 acgtgtcata tcccaggaca gagaactggt cagcgaaacg ctggaaatgc tgcaaacatg
     7501 ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc ctgcagcgcg cggtggaaga
     7561 gaacaccggt gccgatgctg aattgatgct tgcggatatc atcgaagcgc gcgacggtag
     7621 tgaggctgca caggtctata ttacgcgcca gcttcagcgt catccgacca tgcgtgtgtt
     7681 ccataaatta atggattacc acttaaatga agcagaagaa gggcgtgcca aagagagtct
     7741 gatggtgctg cgtgacatgg ttggcgagaa ggtacgtagt aagcctcgtt atcgctgcca
     7801 gaaatgtggt tttaccgcat acaccctcta ctggcattgt ccgtcttgtc gggcctggtc
     7861 aaccattaaa ccaattcgcg gtcttgatgg cctgtaattt ttaaaaaaat ccgactttag
     7921 ttacaacata ctaattattg atcttctatt gtgttccggt accgacggcg cggaaaagcc
     7981 tgccagagga gaaatcgcaa ctgttaattt tttatttcca cgggtagaat gctcgccgtt
     8041 tacctgtttc gcgccacttc cggtgcccat catcaagaag gtctggtcat gacgttaact
     8101 gcttcatctt cttcccgcgc tgttacgaat tctcctgtag ttgttgccct tgattatcat
     8161 aatcgcgatg ccgcgctggc ctttgtcgac aagatcgacc cacgcgattg tcgtctgaag
     8221 gtcggcaaag agatgtttac attgtttggg ccacagtttg tgcgcgaact tcaacagcgt
     8281 gattttgata tctttcttga cctgaaattc cacgatattc ccaacactgc agcgcacgct
     8341 gtcgctgctg cggctgactt aggcgtgtgg atggtgaatg ttcatgcctc cggtggggcg
     8401 cgtatgatgg ccgcagcgcg tgaggcactg gttccgtttg gcaaagatgc accgcttttg
     8461 attgctgtga cagtgttgac cagcatggaa gcctgcgacc tggccgatct tggcgtgaca
     8521 ctgtcacctg cagattatgc agaacgtctg gcggcactga cgcaaaaatg tggccttgat
     8581 ggtgtggtgt gttctgctca ggaagctgtg cgctttaaac aggtattcgg tcaggagttc
     8641 aaactggtta cgccgggcat tcgtccgcag gggagtgatg ctggcgacca gcgccgcatt
     8701 atgacgccag aacaggcgtt ggcggctggt gttgattata tggtgattgg tcgcccggta
     8761 acgcaatcgg tagatccagc gcagacgctg aaagcgatca acgcctcttt acagcggagt
     8821 gcatgatgag tgattccaac agccgtctgg tctactcaac ggaaaccgga cgtattgatg
     8881 agccaaaagc ggcccctgta cgccctaaag gcgacggtgt ggtgcgtatt cagcgtcaga
     8941 ccagtggacg caaaggtaag ggcgtttgcc tgattaccgg tgtcgatctc gatgatgccg
     9001 aactgacgaa actggcagct gaactgaaga aaaaatgcgg ctgcggcgga gcagtaaaag
     9061 atggaattat tgaaatccag ggcgataagc gtgatttatt aaaatcaatg cttgaagcga
     9121 aagggatgaa ggtaaaactt gcaggcggct aacataaaaa gccacggata tatccgtggc
     9181 tttcgaatat tttactgtgt gtattattca gttatttcgt actgaacaga atcaagcgta
     9241 ttataattgc gagtcgataa tcgacattta tttacgatta tttaccgacc tggtgaccaa
     9301 taacaccacc gacggctgca ccacctaatg tacccaacgt actgccatcg gtcagtactg
     9361 caccgcctaa tgcccctgca cctgcgccga ttgcggtgtt gcggtcccgt ttagaccagt
     9421 tagaacaggc actcagagac attgccaggg taattgccag aacagccgcg gtcatttttt
     9481 tgctcgttac aaacataata ctctctcctg aatttatgat tcacggaagt aagctctcta
     9541 taactataat agctaagaat aagccaggtg aaattattcc gtgaaatccg ctcgcgcgca
     9601 gggttatatc acgcaggtga taatgacttc ctgttatatc gctgat
//
LOCUS       DAJOTB010000170         9533 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJOTB010000170 DAJOTB010000000
VERSION     DAJOTB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.170"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            855..1175
                     /locus_tag="OHN28_004149"
     CDS             855..1175
                     /locus_tag="OHN28_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299442.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLLVATVDMSKNFPDRYILLES
                     REIEITVNQPEPIDIIGLQVEQLREQKQKTVADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            1206..3422
                     /locus_tag="OHN28_004150"
     CDS             1206..3422
                     /locus_tag="OHN28_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299443.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIAAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKIWEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVDSDCSSA
                     DISQVSDLMKLPARERFAAVLLFSHDANEVDSELLVQTREYLEMLDNSDTDSEDEVDA
                     FNRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPG
                     KRDEMLPEVKPETASARPYKQTHATLDREIACALLPVAPEKITPSILKAADEIISQDR
                     EDFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRD
                     ANAVEEKPKEAAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLR
                     EQFVTPRHVYDVPENNAVSQREPAAITLAEETPPQEQLSEQVKDLVQNVDALVERIHA
                     EEHKRQNAISAIETELKDSDDTDNLALWKNVFKTDERFTSAFSQNGGGTSINGTYIAM
                     KATREFGPFGIGWGVEVLEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTM
                     KVRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSAD
                     IFMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTANEV
                     NKVFGLIAREVDVHRKAAETKGDKEYSSYLGSRLRRITDIKTERLAALTAAQEQTA"
     gene            3419..3988
                     /locus_tag="OHN28_004151"
     CDS             3419..3988
                     /locus_tag="OHN28_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCQ2299444.1"
                     /translation="MSTAIAIAKEYASLIDLLETADELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNANTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRIR"
     gene            3988..4170
                     /locus_tag="OHN28_004152"
     CDS             3988..4170
                     /locus_tag="OHN28_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299445.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFTPQELITPNQK"
     gene            4380..4643
                     /locus_tag="OHN28_004153"
     CDS             4380..4643
                     /locus_tag="OHN28_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCQ2299446.1"
                     /translation="MKSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFMNPIKFRNLQEVTTRKDDEPDFGAIFNGRKEKEPSR"
     gene            4612..5628
                     /locus_tag="OHN28_004154"
     CDS             4612..5628
                     /locus_tag="OHN28_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2299447.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALTEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKEK
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKTGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(5759..5845)
                     /locus_tag="OHN28_004155"
     tRNA            complement(5759..5845)
                     /locus_tag="OHN28_004155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5809..5811),aa:Leu,seq:taa)
     gene            complement(5858..5931)
                     /locus_tag="OHN28_004156"
     tRNA            complement(5858..5931)
                     /locus_tag="OHN28_004156"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5897..5899),aa:Cys,seq:gca)
     gene            complement(5985..6060)
                     /locus_tag="OHN28_004157"
     tRNA            complement(5985..6060)
                     /locus_tag="OHN28_004157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6025..6027),aa:Gly,seq:gcc)
     gene            complement(6212..6760)
                     /gene="pgsA"
                     /locus_tag="OHN28_004158"
     CDS             complement(6212..6760)
                     /gene="pgsA"
                     /locus_tag="OHN28_004158"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCQ2299448.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6817..8649)
                     /gene="uvrC"
                     /locus_tag="OHN28_004159"
     CDS             complement(6817..8649)
                     /gene="uvrC"
                     /locus_tag="OHN28_004159"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCQ2299449.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8646..9302)
                     /gene="uvrY"
                     /locus_tag="OHN28_004160"
     CDS             complement(8646..9302)
                     /gene="uvrY"
                     /locus_tag="OHN28_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCQ2299450.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2659 a         2412 c         2338 g         2124 t
ORIGIN      
        1 caacaacgct tacccagcgg cagttgttca gaaacacgtt ctgacagccg gaaagacggc
       61 accaaatttt gcgcgtcggc gccaacacgg tgacagaggg aaagacttca ccggcatatg
      121 gcacatgtgt cgaagcggtc tggatggaag cggagcctta acgcgttgtc tccatagcag
      181 gtagccggaa tgtgcaagcc acagccaggt atgagcgatt gattcaccat caaggcgata
      241 cggtgtgacc accagggaag agtcctggct acaacacgag agcgcacttc atcgactcaa
      301 ctttgagctt tgtcgttaaa ttttgaaatg gcggagtgcg ctcccggttg tggtgaacag
      361 gtgtttaacg ggaactccct gcccgttacc cggttcgatt ccgggcgccc atcatcaatt
      421 tgctgtgttt agtctttgcc cagtccgcac gatgggccat tttttcacac agccaggttt
      481 tatcgctgtg cctgagtccc caacaggaga ggccaaaccc gcagcgtgac accagggaaa
      541 gaccggagga agtaccacgc ctgaccagcg ttgaccatga gcctgaccag ctcaaaacag
      601 gaaagaccag cccgggcctg accagccctg tacggtcgta atggaaacat aacgacgccg
      661 gaaacgtaac cggcaccctt tagatagcaa aagacccgca caaggcgggc cagttacccc
      721 gaacggcgac caaaccattc ggatttatca caagtgacca aacttgtgat gaggaaagac
      781 caacgacact gacgctatgg aagctgatca atattcgctg atcggctctg agtatacatt
      841 accaaggagt cgctatggaa gcgcgcacca tcccagtaac actctttatt cattatgcaa
      901 cttcaacttt cagccacgaa aagctgcttg ttgcgactgt tgatatgtca aaaaattttc
      961 cagacaggta catccttctg gaaagccgcg aaattgaaat taccgttaac cagcccgagc
     1021 caatcgacat catcggttta caggtcgagc aactgcgtga acaaaaacag aaaacagtcg
     1081 ccgacgccca acagcgtatt gctgctatcg atgacaaaat ccagcagtta ctttgcatcg
     1141 aatacacgcc agataccgat gagctcccct actaaaaacc actgacctgt aatgaggaaa
     1201 gaccaatgac catttttaac ggcttgttag aagcgaaaaa aggcgcgctc aaaaacggcg
     1261 cgatcccggc gctggccatc gccatcgccg cccctaacaa aaaagttgcc gagaacatca
     1321 tcatcggcaa attgtgggaa gcctaccctg accacggcga caactatttc aaacctaaaa
     1381 tctgggaaga tgccccgggc caaccgcgcc caggcgtcgg tgagtttgat gagacgtttg
     1441 ccacagaaca cagttttgat ggcgaaaaat gggtagttaa cactcctgtt gattcagatt
     1501 gcagttcagc agatatctca caggttagcg acctgatgaa actgccggct cgtgaacgat
     1561 tcgccgccgt tctgctgttc agccacgacg ccaacgaagt cgacagtgaa ttgcttgtgc
     1621 agacgcgtga atacctggag atgctcgata acagtgatac tgacagtgag gatgaggttg
     1681 acgcgtttaa ccgcatcgtt cttgatgcca tggtggcgtg taagcccatc gagtacatgc
     1741 atattgctgg attgaataat ctggtacatg caattttggc aagttgcgat acccaggaac
     1801 aaaacccgac cagttggact atctccaaat ttataaaaaa atgggtagag aatcccggta
     1861 aacgagatga aatgctgccg gaggtaaaac cagaaacagc atccgcacgt ccttacaaac
     1921 agactcacgc cactctggat cgtgaaattg cctgcgccct gttacctgtt gccccggaaa
     1981 aaatcacccc cagcatcctg aaagcggcag acgaaataat cagtcaggac agggaagatt
     2041 ttaaacgctg gtcaatggcc ttgcgcacaa cggatcagat ccttgcctat gaccgcgcat
     2101 cagtattcgg tgttattcag agcgcccccg caaaagacac gtaccacttc ccacaatccc
     2161 tgcgcagtca catcgacaac tggctgcaag ctaacgggca gcgtgatgca aatgctgttg
     2221 aagagaagcc aaaggaagca gcacccaagg atgatgtaaa agtcaccaac cacggcggcg
     2281 gacgattctc aattgatggg atgatgtcag aaacaccctc aaatcagggc gaaaaaagcg
     2341 aagctgcaaa tgctggagaa cgtagtttgc agcagttgcg tgagcagttt gtcacgcctc
     2401 gccatgtgta tgacgtacct gaaaataacg ccgtatcaca gagggaacct gcagctatta
     2461 ccctggcaga agagactcct ccacaagaac aacttagcga gcaggtaaag gatctggtgc
     2521 agaacgttga cgcgctggtt gagcggatcc atgccgaaga gcacaagcgg cagaatgcaa
     2581 tctcagctat cgaaacggag ttaaaggact cagatgacac ggataacctg gcattgtgga
     2641 agaacgtatt caaaaccgat gagcgcttca ccagtgcttt ctctcagaat ggcggcggca
     2701 catccatcaa cggcacttat atagcgatga aagcgactcg cgaatttggg ccatttggta
     2761 ttggctgggg ggtagaggtg ctggaggagc gctttgataa aggcgcacca atagttcgca
     2821 aaaaacaggt tggtgaaaaa atagaatggg atctgattca agacggtgtt ggcgggtacc
     2881 tatgtgaaat gcaccacacc atgaaagtga gagtctggta catattgaat ggggtacgcg
     2941 gtgagtccga ggcttacgga tgcacacctt atatctacga cactaaatac ggaccaacaa
     3001 gcgacggcga agcaccgaag aaatcctgga cggatgctgt taagaaagcg ctatcccctc
     3061 tcggattcag cgccgatatt ttcatgggcc tgtacgacaa tccggaatac cgccagagga
     3121 ataaagctga gtttgatatc aagaatgcca gcgagaatgc ggaagatgct acccgtctgc
     3181 gtaaggaact ggatgagaag cttatcaacg ttgccaatac gctggccgcc gcggtaaccg
     3241 ccaacgaagt aaacaaggta tttggcctca tcgcccgcga agtggatgta cacaggaaag
     3301 cagcggaaac caaaggcgat aaagaatatt catcgtatct gggatcacgt ctgcgccgta
     3361 tcaccgacat taaaactgaa cgcctcgccg cccttaccgc tgcacaggag caaacagcat
     3421 gagcactgct atcgctattg caaaagaata tgccagtctg attgacctgt tagaaaccgc
     3481 cgatgaactg acgccagaga tgatcgccga cacgcttgaa ggcatggaag gagaactggg
     3541 agacaaactg gacgccatga tggtcatctg ccgtaattta cagggcaatg ccaatacctg
     3601 cgctgaagaa atgtctcgcc tggcaacccg taagacgtcc tttgaaggca aggaaaaagc
     3661 aattcgaaag catatgctca cctgcctgca agctgctggc ctggataaac tgaaaaccgc
     3721 aaaaaacaca tttactgatg cacagggggc tatccgggta attatcgata gcaaagataa
     3781 gatcccggat gagtatgagg gcgtgtcact tgttgatgta gaaacggtta ttacaccaaa
     3841 caagcgagcc attaaagaag tgattgaatc tgcggaggcg gttgccgccg aaatactggc
     3901 gcgtggagaa acaccaccag ccgagttatt aaacccggta ccaggcgcac atctggaacg
     3961 cggcgaacgg tcactgagga tacgctaatg cttaaactca cattaaagcg tggtgacgcg
     4021 gttcacctgg tacttgccga tggtaccaat ggcattattg aagcacggag ccgctgcgaa
     4081 cttgggctac acctgccaga aaacattaag gtcactcggg agaaatcggc attcacccca
     4141 caagaactga ttacgcctaa tcagaaataa aaactcacca tcgctagcat tgcgattcac
     4201 cagacaccgg aggatcacaa tgctgcgatg gcaaccggga gcaactctgc tctccgcatt
     4261 tgatatcaaa attggtcgac tgtcggccag cgtcaggaaa cagactttga ccgagtctga
     4321 tattgcccgt gcctgccaga aggcagatga cgcaataagc tacataatga ggaaagacca
     4381 tgaaaagcga tcacgacata atcaccagag aagaaatggt cgagctgacg ggaagcccac
     4441 ttaaatcaaa gcaatgtgag gctcttcgcc gggctggaat tttcttcatg gaaagggctg
     4501 acggacaccc aaaaacaacc tggggccatt tcatgaaccc aataaaattt cgcaatttac
     4561 aggaggtgac gacgcgaaaa gatgatgaac ctgattttgg agcaatattt aatggccgga
     4621 aagagaaaga acccagcaga taactggatg ccgcctcgtg tttaccaggg caaagcggcc
     4681 tacgaattca ggaataaaga taacaaagcg atacgcctgt gcgcattgga tgcaccacga
     4741 tcagccgtat ggctggcata tgaaaaagcg gtcggtgacg aaaaagaaag aaatactttt
     4801 caggcgctca cggaacaatt catgacctcc cctgatttta tggatttggc agtcgaaacc
     4861 cggaaagact acacaaaata ttccggaaaa gttctgcctg tcttcgggaa gatcgacccg
     4921 gataaaatca aacctgaaca tattcggcgc tatatggatc aacgcggttt atcaagccga
     4981 actcaggcaa acagggaaaa aagctttatg tcccgggtat tccgttgggg ttatgaacga
     5041 ggttatgttc agaggaatcc ttgtcaggga gttaaacagt ttaaagagaa agctcgcgaa
     5101 cgttatatta cggatgaaga atatcaggcc gtttatgaag tgtctcctga tgttgttcgc
     5161 gtagcaatgg agattgccta cttatgcgtg gccagacaga gtgatgtact ttcattgcag
     5221 aaagaccagc tgttcgattc cgggatctac attcgtcagg gaaaaaccgg cgttaagcaa
     5281 atcaaagcct ggtcgcctcg tctgcagaaa gcgatagctc tggctcgttc tctgccatta
     5341 aaaacgggaa tcagtagcct gtttgtgatt catcaaacta ccggtggcaa gtatacccgt
     5401 gatggtttta actctcgttg gcgtgatgtc aaagcggcag cacaggaaaa atatcctcat
     5461 ctgcaaatag acttcacatt tcatgatctg aaagcaaaag gtatctctga tctggaaggc
     5521 agcctggaag agaagcaagc aatttccggg cataagaacc cgcgacagac agcagcatat
     5581 gaccggaaag ttaaagtagt gcccgtagtt ggtggccaga gaaaatgaat gatgatgcgt
     5641 ccagagaaaa atcatcttcg gacgcatctt cggaaatgag aaagaagata caaaaaaacc
     5701 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     5761 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     5821 tgtctaccga ttccaccatc cgggctcggg aaaaaagtgg aggcgcgttc cggagtcgaa
     5881 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     5941 aggcctttca gccagacatc cgctgacgcc gatgcctttt aaactggagc gggaaacgag
     6001 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
     6061 attcatcaag caatcagtta atcacttgat tttattatcg tctggcaatc agtgccgccg
     6121 ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt tcaccacttt
     6181 tgatcgtttg ctgaaaattg cgccgaaacg atcactgatc aagcaaatct gcacgcgcag
     6241 cgctcaaata ttgcaacatt gaccacagag tcagtaccgc agccacaaag aaaagtgcaa
     6301 taccggcgta ctcaacccaa atattcggac gccacagcag ccatgccaac gccaccatct
     6361 gggcagtggt tttcactttt ccaatccagg agacagccac gctactgcgt ttacccaact
     6421 ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc gttgctgccg
     6481 gtaaggtcac ccaccagctg tggtaatgct cggttaccag caccatggcg atagccacga
     6541 gaactttatc tgcgacaggg tcaaggaaag caccaaaccg ggtactctga ttccagcggc
     6601 gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg
     6661 caaacggcga ccaggtgaca ggcaaataaa agaccaatac aaagaatggg ataaggatga
     6721 cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt aactatctgt
     6781 tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg accagaagat
     6841 cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga cgctggcgtt
     6901 acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccgacgtt ttggcccgac
     6961 gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt tacggtgccc
     7021 gccaatcgcg tgatcgtgtg attcatcgcg aatatgctgg ataacatgca gcgcgggaga
     7081 atctggcggc aaactaaatc cctcaccttc cggctcaaag aacagcgttt ccagcccagc
     7141 cttacgatct gctcctttgg caacgccaag tagcagcgga tgatttttat cccatgagac
     7201 atccagttcg gcgaaaacat ttttcgcctg cgcaagctgg cctttgccgc cgtcaataag
     7261 gatcacatcc gggatcttac tgtcgtcgat agctttacca taacgccgac gcagcacctg
     7321 attcatcgcc gcgtaatcat cgcccggcgt gatgccagta atgttatagc gccgatactc
     7381 cgcacgcagc gggccgttgg catcaaacac cacacaggaa gcgacggttt gttcacccat
     7441 ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca acacgctggc
     7501 aagcgcggtc agccgctggt gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt
     7561 cgccgcattg gtacgcgcga gtttcagata acgcgctcta tcgccacgag gtttggtttg
     7621 aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg ttttatcgct
     7681 aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc cttgtaaata
     7741 gaactgacct acgaaggttt ccaccacctc gctcagttcc gtaccgccag gcactttcgg
     7801 gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt ggacacaagc
     7861 catacccgca tcgaacgcca ctccaataac gtcaaggtcg tcgccggtat tggaaacaaa
     7921 ctgtttttcg gtgacacgtc gcaccgcctg aatttggtcg cgaatccgcg ccgcttcttc
     7981 aaactccaga ttctggctgg cagtttccat acgactaatg agttgcgtaa gcacctgatc
     8041 atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt cttcttcact
     8101 caccagtcct tcaacacacg gccccagaca gcgccctatc tgatattgca gacacggacg
     8161 cgagcggttg cgataaacac tgttttcgca ctggcgaatg gggaaaatct tttgcagtag
     8221 cgccagtgtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt cacctttggc
     8281 atgcttcgct ccacgatgca tcgccagacg cgggtgggta tcgccactca ggaagataaa
     8341 aggatatgat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataaa gtttgatgta
     8401 gttgtgttcc agtaacagcg cttcggtttc tgtatgagta accgttacat caatttgctg
     8461 gatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga aatagctgga
     8521 aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac cagcatcata
     8581 catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg cgtcaaactg
     8641 atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat tgccaggtga
     8701 gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg gtagctgttc
     8761 accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg gcccttggtg
     8821 atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct ttctgttttt
     8881 tctggttcga tctggcttaa cgccatttgt tgagcgatat cagaagcaat gtaacgctgc
     8941 cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc tttgctgagg
     9001 tagcccgcag ctccggcctg catgactttc gctggtaaag ggttttctgt atggacggta
     9061 agcatgatga ttttgacatc agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg
     9121 ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgc ccggcaccat
     9181 ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt tatatcttcc
     9241 agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag aacgttgatc
     9301 aaaggaatat ctccagaaat agggataacg ctactgatag ttagtcattc gtatattagc
     9361 ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt ctctcgtttt
     9421 tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggcttc cgggatcgtt
     9481 tcacagtaag cccggcgatg aaatgtgaca aaaattgact ttattcagca aaa
//
LOCUS       DAJOTB010000171         9462 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJOTB010000171 DAJOTB010000000
VERSION     DAJOTB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.171"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..172)
                     /locus_tag="OHN28_004161"
     CDS             complement(<1..172)
                     /locus_tag="OHN28_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCQ2299451.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMM"
     gene            complement(391..1368)
                     /gene="epmA"
                     /locus_tag="OHN28_004162"
     CDS             complement(391..1368)
                     /gene="epmA"
                     /locus_tag="OHN28_004162"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCQ2299452.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1693..3501
                     /gene="frdA"
                     /locus_tag="OHN28_004163"
     CDS             1693..3501
                     /gene="frdA"
                     /locus_tag="OHN28_004163"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCQ2299453.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3494..4228
                     /gene="frdB"
                     /locus_tag="OHN28_004164"
     CDS             3494..4228
                     /gene="frdB"
                     /locus_tag="OHN28_004164"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCQ2299454.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4239..4634
                     /gene="frdC"
                     /locus_tag="OHN28_004165"
     CDS             4239..4634
                     /gene="frdC"
                     /locus_tag="OHN28_004165"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCQ2299455.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4645..5004
                     /gene="frdD"
                     /locus_tag="OHN28_004166"
     CDS             4645..5004
                     /gene="frdD"
                     /locus_tag="OHN28_004166"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCQ2299456.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5067..6200
                     /locus_tag="OHN28_004167"
     CDS             5067..6200
                     /locus_tag="OHN28_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2299457.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            6289..6822
                     /gene="blc"
                     /locus_tag="OHN28_004168"
     CDS             6289..6822
                     /gene="blc"
                     /locus_tag="OHN28_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCQ2299458.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6819..7136)
                     /gene="sugE"
                     /locus_tag="OHN28_004169"
     CDS             complement(6819..7136)
                     /gene="sugE"
                     /locus_tag="OHN28_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCQ2299459.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7312..7458)
                     /gene="ecnB"
                     /locus_tag="OHN28_004170"
     CDS             complement(7312..7458)
                     /gene="ecnB"
                     /locus_tag="OHN28_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCQ2299460.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7569..7694)
                     /gene="ecnA"
                     /locus_tag="OHN28_004171"
     CDS             complement(7569..7694)
                     /gene="ecnA"
                     /locus_tag="OHN28_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCQ2299461.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7746..8312)
                     /gene="efp"
                     /locus_tag="OHN28_004172"
     CDS             complement(7746..8312)
                     /gene="efp"
                     /locus_tag="OHN28_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCQ2299462.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8354..9382
                     /gene="epmB"
                     /locus_tag="OHN28_004173"
     CDS             8354..9382
                     /gene="epmB"
                     /locus_tag="OHN28_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCQ2299463.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2415 a         2441 c         2405 g         2201 t
ORIGIN      
        1 ccatcattaa ggcgaatgga ataaagaata acaatgcaga aaatatataa aaggaaatcg
       61 cactataacc cattaagtaa taagccgatg ggctattggc aaatccaaat acagaagtaa
      121 agatcatcaa tatgagtcct atcagactca tcttttttat cgagtgaggc atgaaaccat
      181 ccttcatttc agcgcgtgta taaaacgcgc ccactgaact gcactgccca acattgagcg
      241 ggcagcgtaa aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa
      301 ttgtggcctt tagcggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt
      361 cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac
      421 ttcagccagt gtctccgcgc ccagcgccaa catcaccaga cgatcaacac ctaatgccac
      481 gccggaacag tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg
      541 ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc
      601 acgggcatcc gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc
      661 aaagcgttca gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc
      721 tggaaagtgg tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt
      781 aaacagcaat tgtagaagcg tgtcgcggtc ttcttcagta tcggcaacgt tgctcaaatc
      841 cagtttcgct gccacttccc gcagttgcgt tttgtcggca gagagtgggt caatttccag
      901 ataacgcaag aaagcttgtt gataagaaag gctttctgct gccggacagt ccagcacctg
      961 ttgcaagaga tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca
     1021 ctcaagcata gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg
     1081 gcacagctgg aataccggcc cacaaccggc aaccagcagg cgcttcatat ggtattccgg
     1141 gctggtcatt aaccagagat tcatcccctg cgaatgcccg gggccaacga aacgtgtctc
     1201 aaacgggacc aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc
     1261 cagcactcca cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa
     1321 taagttagga atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc
     1381 agtttttgac aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag
     1441 gaataacaca acggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa
     1501 ttaatcatct tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa
     1561 tttcagactt atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt
     1621 cgcagaccgt aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc
     1681 tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg
     1741 ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc
     1801 tcaaaagtat acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc
     1861 gcgcaggatc atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg
     1921 ttgtgtgagc aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa
     1981 ctggaactgt gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc
     2041 ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg
     2101 ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga tccagcgttt cgacgaacat
     2161 ttcgtgctgg atattctagt tgatgatggt catgttcgcg gcctggtagc aatgaacatg
     2221 atggaaggca cgctggtgca gatccgtgct aacgcggtcg ttatggctac cggcggtgca
     2281 ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg
     2341 gcgctaagcc acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt
     2401 ctaccaggtt ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc
     2461 aacaaaaatg gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc
     2521 gagccgaaaa acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg
     2581 cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg gcgatgtggt ttatctcgac
     2641 ctgcgtcacc ttggcgagaa aaaactgcat gaacgtttgc cgttcatctg cgaactggcg
     2701 aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac
     2761 tacaccatgg gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc
     2821 gccgtgggtg aatgttcctc tgttggtctg cacggtgcga accgtctggg ctccaactcg
     2881 ctggcggaac tggtggtctt cggtcgtctg gccggtgaac aagcgacaga gcgtgcagca
     2941 actgccggta atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt
     3001 ctgaaagatc tggttaacca ggatggcggc gaaaactggg ctaagatccg cgacgaaatg
     3061 ggcatggcca tggaagaagg ctgcggtatc taccgtacgc cggaactgat gcagaaaacc
     3121 atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacttcc
     3181 agcgtgttca acaccgacct gctctacacc attgagctgg gccacggtct gaacgttgct
     3241 gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcgca ccagcgtctg
     3301 gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc
     3361 gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca
     3421 gctaaacgtg tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag
     3481 aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc
     3541 ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc
     3601 attactggat gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg
     3661 gtcctgccgt atggcgattt gtggctcctg cggcatgatg gttaacaacg tgccaaaact
     3721 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
     3781 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
     3841 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
     3901 cccggcgcag atggcgaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
     3961 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
     4021 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
     4081 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tactgctccg aagtctgccc
     4141 gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt
     4201 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
     4261 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc
     4321 tgcgcgaagg cacggcagtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
     4381 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
     4441 ttatcgtgat cattaacctg atcactctgg cggcagccct actgcacacc aaaacctggt
     4501 ttgagctggc accaaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
     4561 caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
     4621 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgatgaaccg
     4681 gtattctggg gcctcttcgg ggccggtggt atgtggagcg ccatcattgc gccggtgatg
     4741 atcctactgg ttggtattct gctaccgcta gggctgtttc caggtgatgc gctgagctac
     4801 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
     4861 gttctgccgc tgtggtgtgg tttacaccga atgcaccacg cgatgcacga tctgaaaatc
     4921 cacgtacctg cgggtaaatg ggttttctac ggtctggctg ctatcctgac agttgtcaca
     4981 ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg
     5041 gccgttttgt atggaaacca gaccctatgt tcaaaacgac gctctgcacc ttattaatta
     5101 ccgcctcttg ctccacattt gccgcccctc aacaaatcaa cgatattgtg catcgcacaa
     5161 ttaccccgct tatagagcaa caaaggatcc ccggtatggc ggtagcggta atttatcagg
     5221 gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac
     5281 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
     5341 acgctattgc tcgaggggaa atcaagttaa gcgatcccac aacaaaatac tggcctgaac
     5401 ttaccgctaa acagtggaat gggatcacac tattacatct cgcaacctac accgctggcg
     5461 gcctgccatt gcaggtgccg gatgaggtga aatcctcaag cgacttgctg cgcttctatc
     5521 aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg
     5581 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgaaaa
     5641 ctcgtgtctt ccagccactc aaactcaatc atacgtggat taatgtaccg tccgcagaag
     5701 aaaagaacta cgcctgggga tatcgcgaag gtaaggcagt acatgtttcg ccaggggcgt
     5761 tagatgctga agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa
     5821 gcaatttaaa accccttgat atcaatgaga aaatacttca acaagggata caactggcac
     5881 aatctcgcta ctggcaaacc ggcgatatgt atcagggtct gggctgggaa atgctggact
     5941 ggccggtaaa tcctgacatc atcattaacg gcagtgacaa taaaattgca ctggcagcac
     6001 gccccgtaaa accgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa
     6061 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
     6121 tcgtgatgct ggctaacaaa aactatccca atccagcgag agttaccgcc gcctggcaga
     6181 ttctcaacac tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctccgctt
     6241 ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc
     6301 cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacaccg
     6361 ccgcgtggcg tgaccgtagt gaataatttc gacgctaaac gctatctcgg cacctggtat
     6421 gagattgccc gttttgatca ccgctttgaa cgtggactgg aaaaagtcac cgcaacatac
     6481 agcctgcgtg atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagaa
     6541 atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg
     6601 aaagtgtcat tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa
     6661 taccgccatg cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg
     6721 ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt
     6781 gatgtcagta agtttatttg ggtacagcag cccggtagtt aatgagtgct gagtttcaga
     6841 ccaataatcc ccaatacgat taacgccaga ctcgccagac gcatcgggtt agcggactca
     6901 ccgagcaaca caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca
     6961 taagccgtcc ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc
     7021 atcgccgtca cggtaataac acttggcgtc aaacgactaa agccgtgggt atatttcagg
     7081 ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca ggacatatca
     7141 ggctccggaa aattggggcc gtccccggtg aaaagacgcg tttgcaggtc gtcctgcaaa
     7201 gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctatgta
     7261 actatcgcag aaaaaacaaa cggcacgaca cagctgtcgt accgttattg cttattgctg
     7321 cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc
     7381 acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc
     7441 aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag
     7501 caaattaata atagacaaca tcgccaaaaa tgacggaaat ctgatgattt ttcggaagag
     7561 aagaaaaatt agctggcagc gcgggagatg gagttgccga ggtgtttgat atcttcgccg
     7621 aaacctcgag cggtgttaca gcccgtaagc agcgtgctgg caagcaaaac aagaacgata
     7681 aggcgtttca tcatctctaa cctgaataag tgatggtgca gcctgcaggc cgcaccacaa
     7741 ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg
     7801 ccgatttgta caaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg
     7861 ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatccacgat ttccagttca
     7921 acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct
     7981 gcctgatcca gcaaccattt ggcgttgtcg ccaattgctt ttgcatcagc agacagctgc
     8041 tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg
     8101 ttcatatcga caacatcagc accttcagcg gaatcagtag atttgaaggt tttttctacg
     8161 cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt
     8221 tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca
     8281 gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta
     8341 gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt
     8401 aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga
     8461 cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc
     8521 ccgctcattt atcgatcgca tggagaaagg caatccgaac gatccgcttt tgcgtcaggt
     8581 aattacctcg caagatgagt ttgttgtcgc gcccggattc tccaccgacc cgctggaaga
     8641 acagcacagc gtagtgccag gtttgttgca taaataccac aaccgggcgc ttttgctggt
     8701 caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga
     8761 aaatcagggc aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga
     8821 actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga
     8881 ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg
     8941 tctgccgatt gtgatcccgg cacgcatcac agatgcgctg gttgaacgct tttctcattc
     9001 tacgctgcaa atcttgctgg tgaatcacat taaccatgcc aatgaggtag atgaaacatt
     9061 ccgtcaggcg atggctaagt tgcgccgggt aggcgttact ttgctgaacc agagcgttct
     9121 gttacgtggt gtgaacgata acgcgcaaac gctggcaaac ctgagtaatg cgttgttcga
     9181 tgcgggtgtg atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt
     9241 tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg
     9301 atatctggtg cctaaactgg cgcgagaaat tggcggcgaa cccagcaaaa caccgctgga
     9361 tctccagcta cgccagcagt aactgattaa acagggaagt ttaatctttg cccgttgcat
     9421 aacctttaat tattcatata cattgctgtt attactattt at
//
LOCUS       DAJOTB010000172         9399 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJOTB010000172 DAJOTB010000000
VERSION     DAJOTB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.172"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(147..719)
                     /locus_tag="OHN28_004174"
     CDS             complement(147..719)
                     /locus_tag="OHN28_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCQ2299464.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTAIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASHQSV"
     gene            complement(793..1293)
                     /locus_tag="OHN28_004175"
     CDS             complement(793..1293)
                     /locus_tag="OHN28_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCQ2299465.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(1290..2024)
                     /locus_tag="OHN28_004176"
     CDS             complement(1290..2024)
                     /locus_tag="OHN28_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCQ2299466.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENAHLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRMTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            2577..2843
                     /locus_tag="OHN28_004177"
     CDS             2577..2843
                     /locus_tag="OHN28_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2299467.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            2840..3439
                     /locus_tag="OHN28_004178"
     CDS             2840..3439
                     /locus_tag="OHN28_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCQ2299468.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRGNPSNSQATY
                     DAPCVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGISTPVWAIAIERGNSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRTDRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            3432..3719
                     /locus_tag="OHN28_004179"
     CDS             3432..3719
                     /locus_tag="OHN28_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCQ2299469.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            3712..4167
                     /locus_tag="OHN28_004180"
     CDS             3712..4167
                     /locus_tag="OHN28_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299470.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFRRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWQEIPRTEPDPEITPDNSYRNYL"
     gene            4303..4623
                     /locus_tag="OHN28_004181"
     CDS             4303..4623
                     /locus_tag="OHN28_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCQ2299471.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            4638..6971
                     /locus_tag="OHN28_004182"
     CDS             4638..6971
                     /locus_tag="OHN28_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCQ2299472.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQVGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATA"
     gene            7341..8378
                     /locus_tag="OHN28_004183"
     CDS             7341..8378
                     /locus_tag="OHN28_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HCQ2299473.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            8948..>9399
                     /locus_tag="OHN28_004184"
     CDS             8948..>9399
                     /locus_tag="OHN28_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299474.1"
                     /translation="MGHGTCKAANSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLFQNNNALYLFENDNKIELRMGHDSFFSIETIDKINKENIKGIKLKN
                     ETTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPCFIIG"
BASE COUNT         2238 a         2518 c         2420 g         2223 t
ORIGIN      
        1 gttttaatgc agttgcactg acacgctcag accgcgccgg gaatggtgcg gtttgcagaa
       61 aatgaggcag gaaaacgcat gcaaagccat gcaccttatc gatgcatggc ttttttcagt
      121 aaaaacgggc ggattttcgg ggagttttac acagactgat gtgatgccag ttgcgcactt
      181 ttacgcgaaa aaatcatgtt ctgcgcaggg gtgaattttt cacggctgtc atccaccgaa
      241 gccgcgtcag gtctgaatcc aatggccgtt aaaatgtcgc tatcctgtgc ggaataatta
      301 attttttcac ccttcgcaag ccaggacagg agggcttcac gcagggcatc tgtggcacgc
      361 tgtatggcac agtttcgggc aatggccgtc agctcactgt agcccatcag ctccggtgcc
      421 agtgccgccg ccagtgctgt gccgtgctgc tgcataaaat cattcagccg gtcgcggatg
      481 ctgatgtgct gaacagcttc atgcgaacga atataacgac cggcagcctg attcacctgc
      541 cattttctga cttcgataat attgcgtaat tcgtccaggc gactgacgtt tctgccttcc
      601 ccggacagaa gccgcagata ttcctgttcg gccgcagcca gctcattttt gcgttgcagc
      661 catgctgctt tgttattctg acaggtgtca aaggcctgct gtaaggctgt gctttccatc
      721 gttatctctt tctcatcatg ctgaagaata aaaatacggt gtgcggcgac ggccggtgtt
      781 aaccggcagc cctcattcca gacgcagcga atatgattgt gtttttaagc gtactggcgg
      841 cagttcctgt ttttcatgca ggcgttctgc cagttcgtcc ggcgtgaccg ggcggacaat
      901 aaaacggttg atggtctgaa gcgttttaaa caccagacca catcccggat ccgtgcacac
      961 ataaaaacgc tcggtgactt cctgagacag acgccgcgat gttcttgaca gtgcaaggcc
     1021 tttacatctc cgacaacaat atccggtaac aagcattctt ttcgggcgtt tcatactgcc
     1081 ggaggctgac gtcagtgaat cgcggtatct ctgtttgcct gaaatgtatt ccattcctga
     1141 atctttactg tcagagaaaa agctttcact cgcttcaaat gtcgcagagc aataaatatt
     1201 ccggcactgt gcaatcatta tcttggtgcc atcgtccatg aaatgtgcgc gacgggtgtg
     1261 agcaacatgt ccacacgacg ggcagtaaat catgacagca gtcctctggc cttaagctct
     1321 gcaccctgct ggtctatttt gtcctgccac accttgcgct gtgccggtgt gcctgccacc
     1381 tcataatcca tgtgcgggag tgttgccgct gacagtccgg tcatccggag aaccggctcg
     1441 ccggtgaggc tgatttgcat ctgttttatt ttctgttcca gcgatgattt cacctgctgc
     1501 atgacagcct tttccggcgc gacgtagccc tgatggccgg tggtgttggc gagcggattt
     1561 tcctgtacca ggatgctcag atgcattgcc cggacaagcg cctcacaggt ttcattcagg
     1621 gcgtgttcca gctcatgctc tgcatacaga ctcagcaggt gatgatgtgc cttccggtag
     1681 gcggtggccg tgctgtcaca cgcccctttc aggcgttcac gttcaaaatt cagcaccacg
     1741 gccagattgt catattcctg taccagctcc cggcgtgcca cgcgctcaat gtggcgctgt
     1801 ttcagctcat cgctcaggac accaccggct gcacgaaagg ccgtgcgcca gtcgtcagcg
     1861 tcgtttccgt cggcctgcgc cagcgcattt ttttcctgct ctgcccgttc aatggccgtg
     1921 acggtctcat ccatcaggtg ggcgttctca agatgggcgg ctctggcctt ttccagttgt
     1981 gccagcgcgg gttgcagata ttcagggatg gtgttgtcag acattttccg gctcctcgtc
     2041 acttcaggtt aagaaaattg tgacgtacac cggacaacaa cacgacgcat tgcagatgtg
     2101 ccagccctga cacaggagac tcatcctcag accggcaagc caggaaaagg tcgcaggaaa
     2161 aaccggctta ctgtttgttt ttttatattt tactgttcac ctctgttcac cataagaaaa
     2221 aagataagta atacagtaag ttaaagggtg aacaatcgca gtaatgactg ttcaccgtct
     2281 gttcaccact gttcacccgc tcatggactt tttgtgctgt ttactactgt ttgtttttat
     2341 taattcgccg ggaatgaata agaagaaacg atttgtattt cactataaaa attacgaatt
     2401 gctttaagtg gctttaaatg actttaagtg gagatgaaca aaaaatacac aagcattgta
     2461 aggctgccgg aacaaatccc ccctgttgcg tctgctgaaa atattcacaa aataaagcgc
     2521 tacccgaagc cggacggact tatccggtgc tgtatggaca ttaacgaggt agcccgatgc
     2581 aagctgtttt ttcttccccg tctcccgccc ctgtgacgcc actgatgccg ctgccggaca
     2641 tcacacagga gcgtttttta cgtcttccgg aagtgatgca cctgtgcggc ctgtcacgct
     2701 cgaccatcta tgaactcatc cgtaaggggg aatttccgcc gcaggtgagt cttggcggta
     2761 aaaatgtggc ctggctgcac tctgaagtca ccgcatggat ggccgggcgc attgccggac
     2821 gcaaacgggg gtacgacgca tgatgatgcc cgctctgcaa aaactccctt tttctggctt
     2881 gcctttttcc ggcatttgcg gatatagttt ttccgctgcc gcaaaatcgg cagccgggcg
     2941 tggaaacccg agcaattcac aggcgacata tgacgcgcca tgcgtctttt tttgtgtcgc
     3001 aatcaacgcc acagagcgcc agattatggt gtggtgtgtg gtcagtcgtg cagatggcat
     3061 tccgtgcatc ctgcccgcgt ccgctcatta tgccgcagag tcaatggtag ctcaggcggg
     3121 gcagcctccg ggctggccgg tatcctgtga agccggtatt tccacccccg tctgggctat
     3181 cgccatcgag cgtggaaact ccggcgatag cgttatttgc tattcacagg aggctgccat
     3241 catggctaca acccttaccc cctcacaccc tgaatttgtc tttgtgtttg cggctgtccg
     3301 tcgcacagac cgtcatcccc gtatctgtat gcttcgcacc gtcgccggtg atgaacgcag
     3361 tgcccgccgt tcccttgtcc gtgactatgt gctctccctt gctgcccgtc tgccggtggt
     3421 ggaggtgtcc cgtgcgtaat aaaaaagccc ctcagaccgt ctcagcgcgt catgacgccc
     3481 gtgaacacct cagcattgag gcttaccata agctcaaccg cgccagcgcc gtatcccagt
     3541 ttgttggggg tgatttgatt caccgtgaac tctccggcct gcatcagctc tacattccgc
     3601 atattttcag ctacctgaat gaagatattg attttgtgct gaatgagctg aaagccaaag
     3661 gcctgtgccg cgattttctc gcccagcaga aagaccgggg agacaggacg catgtttgat
     3721 tttccccagc ccggtgagat ttaccgttct gccggttttc ccgatgtggc cgtggtcggc
     3781 attctggaag acggtattcc gtgggaaatg ccgtaccgct gcccggacat tgtctggaac
     3841 ccgtaccgcc gtaaattcag tatccttgtg cgtatcctcg ctgacgggcg caccacagac
     3901 atcccgctgg ggcgttttct gcgggaattt acctgtgacc gtcctgacct gttcagacgc
     3961 agccccgtaa accggcatgc ggtactgaaa gaaatggccg gagacccgga attacagaaa
     4021 tggcgggaga aatatctgga tatttacccg caggacactg ttccggtcag ccgggcggca
     4081 ccggtggcgc gggaatggca ggaaattccc cgcacggagc ctgacccgga aatcaccccg
     4141 gataacagtt accgcaatta tctgtaatta aaaaacgaca cccgaaaaat taaatgtgcg
     4201 tattcgcgca gggatacgca cgtcttcagg agacgcagat atgccttatc agttaatgca
     4261 accggcacgg aatgcagtca tctgtcacag ggaggaaagc aaatgaaaac acccttaccg
     4321 cccgtcttac gcgcagccct ttaccgtcgc gctgtcgcct gtgcctggct gaccgtgtgc
     4381 gaacgtcagc accgctaccc gcatctcacc cttgaatcac tggaggcggc catcgccgct
     4441 gagctggaag gcttttatct gcgccagcac ggtgaggaaa aagggcgtca gatagcctgt
     4501 gccctgctgg aagatttaat ggaatccggc cccctgaagg ccgcgccgtc gctgtccttt
     4561 ctcgggctgg ttgtgatgga tgaactctgt gcccgtcaca taaaagcgcc agtactgcac
     4621 tgaaggagaa caacaccatg aaaatgaacg taaccgccac cgtcagccat gcgctcggcc
     4681 actggccgcg tattctcccg gcgctgggga ttcaggtgct gaagaaccgt catcagccct
     4741 gtccggtctg tggcgggagt gaccgtttcc gttttgatga cagggagggg cgcggcacct
     4801 ggtactgcaa tcagtgtggt gccggtgacg gcctgaaact ggttgaaaag gtgtttggtg
     4861 tttccccgtc cgatgcggcc gcaaaggtgg ctgccgtgac cggcagcctg cccccggctg
     4921 acccggcagt gacggctgcc gccggtgctg aaacagacgc tgcccggaag aacgccgccg
     4981 cactggcaca aaccctgatg gcgaaaaccc gtcccggaac cggtaacgcc tacctgaccc
     5041 gcaagggctt tcccggccgg gaatgccgga tgctgaccgg cacacacaga gccggtggcg
     5101 tgagctggcg tgccggtgac cttgtggtgc cactgtatga cgacagcggc gaactggtta
     5161 accttcagtt aatcagtgct gacggccgta agcgcaccct gaaaggcgga caggtcaggg
     5221 gcacctgtca cacccttgaa ggacagaatc aggtcggaaa acgtctgtgg atagcggagg
     5281 gatacgcgac cgcacttacc gtacatcacc tgaccggtga aacggtgatg gtggcgcttt
     5341 cttccgtgaa cctcctttct ctggccagcc ttgcccggca gaagcatccg gcctgtcaga
     5401 ttgtccttgc tgctgaccgt gacctcagcg gtgacggcca gaaaaaagcc gccgcagccg
     5461 cagatgcgtg tgaaggtgtt gttgccctgc cgccggtctt cggtgactgg aatgatgcct
     5521 tcacgcagta cggcggggaa gccacccgta aggccattta tgatgccatc cggccaccgg
     5581 ctgaaagccc gttcgacact atgagcgaag cggagttttc cgccatgagt accagcgaaa
     5641 aggccatgcg tatctatgag cattacggcg aggcgctcgc ggttgatgcc aacggccagc
     5701 ttctgtcccg ttatgaaaat ggtgtctgga aggtgctgcc accacaggac tttgcccggg
     5761 atgtggccgg gctgtttcag cggctgcgcg cgccgttctc ctccgggaag gtggcctccg
     5821 tggtggacac cctgaagctg attattccgc agcaggaagc cccctcccgc cgcctgattg
     5881 gctttcgtaa cggcgtgctc gacacgcaga acggcacgtt ccacccgcac agtccgtcac
     5941 actggatgcg caccctgtgt gatgtggatt tcaccccgcc ggtggaaggg gaaacgctgg
     6001 aaacccacgc ccccgcgttc tggcgctggc ttgaccgtgc cgccggtggt cgtgcggaaa
     6061 aacgcgacgt gattctggcc gcactgttta tggtgctggc aaaccgctac gactggcagc
     6121 tctttctgga ggtgaccggt cccggcggca gcggcaaaag tatcatggcc gaaatagcca
     6181 ccctgctggc cggggaggat aacgccacgt cggccaccat cgagacgctg gaatccccgc
     6241 gtgaacgtgc cgcgttaact ggcttctcac tgatacgcct gccggaccag gaaaaatgga
     6301 gcggcgacgg tgccggactc aaggccatca ccggcggcga tgcggtgtcc gttgacccga
     6361 aataccggga tgcgtactcc acgcacatcc cggcggtgat tctggccgtg aacaataacc
     6421 cgatgcgctt caccgaccgc agcggcggcg tgtcacgccg gcgggtgatt attcacttcc
     6481 cggaacagat agccccgcag gagcgcgacc cgcagcttaa ggacaaaatc acccgcgagc
     6541 tggcggtcat cgtgcgtcac ctgatgcaga agttcagcga cccgatgctc gcccggtcac
     6601 tgcttcagtc ccagcagaac tcagacgagg cactgaacat caaacgggat gccgacccga
     6661 cgtttgattt tatcggctat ctggaaaccc tgccgcagac cagcggcatg tatatgggga
     6721 acgccagtat catcccgcgt aattaccgta aatacctcta tcacgcctat ctggcctaca
     6781 tggaggcaaa cggctaccgg aatgtactca gtctgaaaat gttcgggctg gggctgccgg
     6841 tgatgctgaa ggaatacgga ctgaattatg agaagcgcca taccaaacag gggatacaga
     6901 ccaacctgac gctgaaagag gaaagctacg gcgactggct gccaaaatgt gacgaccctg
     6961 caacagcctg acccacctga ccggcatctg ccggtctttt ttttatcccg acatccccga
     7021 aggtgaacaa tccactgttc acccttcacc gtatattcac ccgttatcac actgaaatta
     7081 aaagagaaaa acgaaaggtg aacagtgtga acagttcttt cgaaaaaaaa atttttttcc
     7141 tgagttaacg tgcgtgaaga tacgtgtaac aattaatcga tagatattgt atgtataagg
     7201 atgtgtataa atgaattaga atagaaaact ataaacgatt tgaatcaaat ggttatatgg
     7261 caattttgac tcctgtgatc ttccgccaaa atgcctctcc tgaactctcc cgacgtcaac
     7321 aacactcctt cggaggcaac atgtcagaca aagacttaac ccagccccca gcaggcgagt
     7381 ttattatgtt tgccagcggt gacggaagag tgcgtgttga atgtcgcttt gagtcagaca
     7441 caatctggtt atcccaggcg gcaatggctg aactgtacga taaagatgtt cgcactatta
     7501 acgaacattt aatcaatata ttttccgaag gtgaacttgt tcaaaactca accatccgga
     7561 aattccggat agttcgccag gaaggaaaac gtcaggtttc cagagagata gaccattaca
     7621 acctcgaagc cattctcgct gtcggctatc gcgttcgctc gcctcgaggc acgcaattcc
     7681 gccagtgggc cacccagacg cttcaggaat acctgatcaa aggttttgtg atggacgatg
     7741 agcggctgaa gaatccgcct gtcggttcat cggttgtgcc cgactatttt gatgagatgc
     7801 ttgaacgcat ccgcgatatt cgcgccagcg agcgtcgggt atatttgcgg gtgcgggaga
     7861 tctttgcttt agctgccgac tatcagccat cgcttaaaga aaccacgcaa ttttttcaaa
     7921 ccatccagaa taaactgcat tttgcctgta ccgggcatac cgctgcggaa ctcatccaca
     7981 aacgagcaga tgccagccag ccgcacatgg gcttgaccag ttataaaggt gaagaggtac
     8041 gtaagggcga cgtgacggtg gcaaaaaatt atctcaccca ggatgaggtc agcgaactta
     8101 atcgcgtggt taatatgtgg ctggactttg ccgaggatca ggcccgtcgt cgtcagcaag
     8161 tctttttgcg cgactggcag gataagctgg atcagttcct gcaatttaac gaccgagagg
     8221 ttctacaggg cgctggtaaa atcagcaaga aaatggccga tgaaaaagcg caggcggagt
     8281 atgttcagtt tgccgaacaa caacggcgct taaaagaagc cgaaggcgag aaggatatcg
     8341 ctggtttgct acagtggaat aaagagtcga aaaagtagcc cggaatcccc ccaaacgcca
     8401 tacgcaaaac catttggggg tacttatggg gttactcatt tatcaagttc aaaatataaa
     8461 cattaaatca ataacttaac catcaaatgc gactcctgtg atcgccaaaa tgcctcttca
     8521 gtgatcccca tcctgcttat ttctgagcaa cctgaccctg ctgatgtatc tggtagtatg
     8581 acagagtgat tcatttttct tgccgagtgt acgaaaacgt cttccttgcc accgaccatc
     8641 aaccgtcact caaaaaaacc tctctttttt caagctatca agaataagtt gcattttatc
     8701 tgcaccggac accagccatc catatatggc ttttacctgc tacaaagatg aagaagtgcg
     8761 taaatgtgat gattaaaaca atcaaagaaa aattacaggt gcaataattt cactataaca
     8821 ttataatcca tggtgaaaat aacactgcac aaggattgtc atcatcaaac tcatcggaac
     8881 tattttttct gtcgtcattg gtgtaatact gattgtaagt atcctttcag gagattattt
     8941 ttgccgtgtg gggcatggaa cttgtaaagc agccaacagc gctaggcatt taattgaaag
     9001 taaagagaaa aacagaagta gtcgcgtcaa agtctttagc acacatatta atttgagtca
     9061 tccagcggca tcctggctag caactcaaac ttgcgctaac aatatattca atctgtttca
     9121 aaacaataat gccctttatt tattcgaaaa cgataataaa attgaactgc gaatggggca
     9181 tgattctttt ttctctattg aaacgattga taaaattaac aaagaaaaca tcaaaggaat
     9241 aaaactaaaa aatgagacaa catatgtttc tcttcctaat ccaaccattt taataaccac
     9301 aacatcaaca cctgaacgac agttttctat ttctgttctg gatgatgata acaaagaaaa
     9361 tctacctacc gataccgcac cttgttttat aattggtat
//
LOCUS       DAJOTB010000173         9294 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJOTB010000173 DAJOTB010000000
VERSION     DAJOTB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.173"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            161..661
                     /locus_tag="OHN28_004185"
     CDS             161..661
                     /locus_tag="OHN28_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299475.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLPADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQEDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
     gene            674..1447
                     /locus_tag="OHN28_004186"
     CDS             674..1447
                     /locus_tag="OHN28_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299476.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            1614..2747
                     /locus_tag="OHN28_004187"
     CDS             1614..2747
                     /locus_tag="OHN28_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HCQ2299477.1"
                     /translation="MHFYVDETGQTGRNLFDKTQPVLSYGVLSSDANLDKVAEADLAV
                     IRKTLGVQRLHAAELGLHRLSDLVDTLLVLQKKHRIRFDIWQVVKRDHAIISFFDQVF
                     DQGMNPAVPWSAYWTPLRYPLLLNLASLFDDELASNAWTARLEAHDERASELFCTVSD
                     ELISRTAASALDHRSKQLITDALNWASANFEQLGYNCKTNKERLRIMPNMIGFQSVLH
                     GICSRLGAPERKASIIVDQQSQFNTTQRELNEFYYQIRDMPWELGPGLPVMNMKNMPA
                     EPLVFQSGTKSAGLELVDIYLWTFKRFMEDKALTKPLSRLVYTNLKTARTNSVSIQSV
                     ASRFKELLGKLPVPSAEIMRQAQELRDFDEARRMPYVVSGSPD"
     gene            complement(2781..3293)
                     /locus_tag="OHN28_004188"
     CDS             complement(2781..3293)
                     /locus_tag="OHN28_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299478.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            3859..4077
                     /gene="ccdA"
                     /locus_tag="OHN28_004189"
     CDS             3859..4077
                     /gene="ccdA"
                     /locus_tag="OHN28_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCQ2299479.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            4079..4384
                     /gene="ccdB"
                     /locus_tag="OHN28_004190"
     CDS             4079..4384
                     /gene="ccdB"
                     /locus_tag="OHN28_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCQ2299480.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            4385..5191
                     /locus_tag="OHN28_004191"
     CDS             4385..5191
                     /locus_tag="OHN28_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCQ2299481.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPITPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVVMLKQLS"
     gene            complement(5871..6602)
                     /locus_tag="OHN28_004192"
     CDS             complement(5871..6602)
                     /locus_tag="OHN28_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2299482.1"
                     /translation="MGELKTTHTIMTMTISTAKKSVNISQSNELTEAAYYLPLQAKRV
                     LWLCLIQCYPLKDDPDSVSPVFTVLVADYQKYFKVSVDTASSDVKKGVTALAESSVVF
                     YPKDGEFDEIKRPWLAEAGLKKGRGKWQIEFNYKVMPYLMGLTSQFTTYSLYDCGKIN
                     SVRAIRLYESLCQYRSSGVWVTTQEWLSERFMLPESQRSNFAEMKRTFINPALKKINA
                     NTPLKAAMTQNEDGRLVFTIVDSKN"
     gene            7223..8428
                     /locus_tag="OHN28_004193"
     CDS             7223..8428
                     /locus_tag="OHN28_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2299483.1"
                     /translation="MRRDYGSVGTIALRASALLQAMSRDIEAQRKEFNLTQYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEDSGYEFNKKQVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDIHKEPYVIFVVNLKGGVSKTVTTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNDLDAETLREAVIRPTIIPGVDIIPASIDDGFVASQWE
                     SLVTEHLPGLKPSEVLKKTIIDRIAGDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTGKRDHETSHSLAREV
                     YASNILDASLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            8425..>9294
                     /locus_tag="OHN28_004194"
     CDS             8425..>9294
                     /locus_tag="OHN28_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCQ2299484.1"
                     /translation="MRKVLARGRVLGNSNSEFARMLESDGDVKTFTLKSGKQAKFVKT
                     VVLSGEIESKTFVDSSVNGRDQAMLSPESVSDISRTIKLQQFFPAIGREVNGRIEILD
                     GTRRRAACIYNNVKFEILVTKDDIELSDARQLAKDIQTAREHSLRELGKRLEVTYGTS
                     MTKEEIAQAENLSPAKVTRAFQAAAVPDEMVAVFPVINDISLSDYHFLLKLAEEANNK
                     QRSVTELMEKVQHRLKTMPDYPAIDKSKILAVIRAESKSLIVRPTRTVQTEKLREFSD
                     RNQFARKKIDSKKR"
BASE COUNT         2378 a         2198 c         2312 g         2406 t
ORIGIN      
        1 gtcatatgca gatgccaaac atatggatat gcatggtatc tgcatgtttc tctctttttt
       61 caccgggatc cgaaaaatat catagtagga aatgccgtac ctgagccgtc aggctggccg
      121 gatgatatag tggattctcg aaccggaacg ggagggttaa atgggaaatt attcagattt
      181 tgagaccgat tttgttcagc gaacactggc gcttatcgat caatataacg aaatgattga
      241 ggtgctgggc aaaccattca gggagcagta caactacacg ttgaccctga attgcctcct
      301 gggtcttatc gtgttaccga aagagagagc gctctctttc ctgcctgccg accgtttaac
      361 gcggcaactt aaagctgaaa tgggattaca tgagtcacag ctgcccggac cggaaatgaa
      421 cctgcgggag ttgatccata agatgcggaa ttcagttgct catttctgcg tccaggtaga
      481 gtcagccagc gatgcacacc ttgtagactg gattgttttc agggaatctc aggaagatgg
      541 agaagtctac gccagcttca gcgctccgga gcttttaccc tttctgaaat attatgcgac
      601 cctgctgctc gacaatatgg cccgccgtcg agcccccgat gttaacatat tggatttgta
      661 agggatttac accatgttga ttctcgattg ctcctcacga actcaggcgt tgcatacatt
      721 atctgccggg tttggctgtt cccctgaaaa gctgaaaaaa gtgctgcttt cgctcgatct
      781 tgaaagtatc tacgagctta atcctcgcca gttggttgat gcacctcagt atctgagaga
      841 atacgtctgt gcggaactgg gcgaaccggg accattcacc cgggctctgt ggtttcatgg
      901 cacccggact ttcgccggta atacttttcc ggcggggtta ctggcgctaa atcagtcaga
      961 atccctcgcc atgaaaatgc tgcttgacct ggccccgaat gagatggtcc gtacacacct
     1021 gaaggaatgg gatgttccgg ggggcgttcc ggatgagatg tttcagctca gaaccggcga
     1081 taaaatccac tgggggcctt ttggccattt agtccgggaa ttacatttta acgccagtga
     1141 aaatggactg catgattacc tctggctgcc ggagctggta gaggatgtct gtaaggccta
     1201 ccagaagaaa tacggccacg atcttaaacc gcattatctg agcgtgcttc atccctgtat
     1261 tgtctggttc gaggcggata ttgtttatga aaaaggagtt cttgaaacgg ctctgtcata
     1321 tgcctataca tcagtccgtg acctcccgcc ggacggaaac gcaactttcg gcatagactg
     1381 tgacgggaaa tctgtgtcta ggtcggccat tgccagaatc gagtttctgc agcccgggca
     1441 gatgtaatcc ctaaaaaacg ggtctgtgat ccggcccgtt tgttcattgc cgtgaaccca
     1501 gtattgaggt cattaaagcc ttaagtggat cacttgcagc tggctgcaga aaggttttac
     1561 attgacccgg attatgccgt ttcccttcct cctgcctggt aaaggatctc tgaatgcact
     1621 tttatgtgga tgaaacaggc cagactggcc gaaacctttt tgacaaaacc cagccagtcc
     1681 tctcctacgg agtgctgtcg tctgatgcta atctggataa ggttgctgaa gcggatctag
     1741 ccgtaatacg caaaacgctt ggcgtgcagc gcctgcacgc tgcggaactg ggcttgcacc
     1801 gcctttctga ccttgtagat acgctgctgg ttctgcagaa aaagcaccgg atacggtttg
     1861 acatctggca ggtagtaaag cgcgatcatg ccattatttc gttcttcgac caggtatttg
     1921 atcagggaat gaatccagcc gtaccctggt ctgcatactg gacccctctg cgctaccccc
     1981 tgctgctgaa tctggccagc ctgtttgatg atgaacttgc ttcaaacgca tggacggcaa
     2041 ggctggaggc gcatgatgag cgtgcgtcgg aacttttttg taccgtcagt gatgaactga
     2101 tcagcaggac ggcagccagc gcgctggacc accggtcgaa gcagctgatc accgatgccc
     2161 tgaactgggc gtcagccaat tttgaacagt tgggctataa ctgtaaaacc aacaaggaaa
     2221 ggctgcggat tatgcccaat atgattggct ttcagtccgt attgcacggg atctgttccc
     2281 gactgggggc accagaaaga aaggccagta tcattgttga ccagcagtca cagttcaaca
     2341 ccacccagcg tgaacttaac gagttttact accagatacg ggatatgccc tgggaactgg
     2401 ggcccggatt acctgttatg aacatgaaaa atatgccggc tgaaccactt gtattccagt
     2461 ccggtaccaa gagtgcaggt ctggagttgg tagatattta tctctggaca ttcaagcgat
     2521 tcatggaaga caaggcgctg acaaagccac tttcccgcct tgtttatacc aatctgaaaa
     2581 cagccaggac caacagcgtg tcaatccagt cggttgccag tcgatttaag gaactcctgg
     2641 gtaaactccc ggtaccaagc gcagagatca tgaggcaggc gcaggagctg cgggactttg
     2701 atgaagccag acgcatgcca tatgtggtgt cgggatcacc cgactgatgc cggatattat
     2761 ctgctttcaa ctgccccctc ttaggggcag gttttcaggt taaccggcag ctgtgatcca
     2821 cgacgctgct tacagtcatg ctgcgatgcc agaatgtcag caacctgata atcggtcccc
     2881 atcgccacat cgtgcaatag caggaaaaag tccccgtcac tgccgcccct gcgcgaatcc
     2941 agataccgct gcagccatcg ggcatgataa agaacgtggc gctgttcgcg ctgcagccat
     3001 ttcagcggca gacgggacgt catatcgtca aggatacgca tttcctgatg aagcggcgca
     3061 ataacagcgg aaaattcctc tctcagtatc cgggcaggtt tcttctgtcc gaacagcgcg
     3121 gccattttgc tcaggacatc ctcgatcgcc cggctgactt tctgggcctg atcacgggag
     3181 gcctgataac gcgtaaacca aaacttacag tagaaccccg cagcggcaag cagcgccgta
     3241 aggatcggtc caatcagact tgtgtccaca atattctcct taaagatggt catgaggcca
     3301 cctgaagcag ccgatgacgc aaatatactc cgttttagca attagtgcta tgcgagaatg
     3361 caaaaagtgc tattcgttgt ttttggtgtt gacattttaa tttatgtgtg agttttttgt
     3421 tttctttcag ttttctccac tgagatccga aaaccccaag ttacggatcc gcggatcctt
     3481 cgctgccgcc cgctggtccg ctttcacagg atgtggtgtc ccgctccgct gacatgacac
     3541 tgtatgttgt gtttcttccc gtgttcccga gccagtcatc agcggcagaa aagatcgctt
     3601 cgcttattca ctgatcctga ttcacttccg gaaaatcctg aatccgataa aaatcggatc
     3661 atagaaaaac agtcatcctg taatttatca tggttactac attgcaggtt attattgtca
     3721 tgattacttt tcaggtcagt tattgatggt ggtgttaata atagctggat aacaatattc
     3781 cgttgccgga ggcatgttat agcgtaatac tggtgtatac ttaatggtgt acagagagta
     3841 tgtaagatga ggtgcattat gaagcagcgc attacagtca ccgtcgacag tgacagctat
     3901 cagttgctca aggcatatga tgtcaatatc tccgggctgg taagcaccac cctgcagaat
     3961 gaagcccgtc gtctgcgtgc cgaacgctgg caggcagaaa atcaggaagg gatggctgag
     4021 gtcgcccggt ttattgaaat gaacggctct tttgccgacg agaacaggga ctggtgaaat
     4081 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtcca
     4141 gagtgacatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     4201 gttgtcagat aaggtctccc gtgagcttta cccggtggtg catgtcgggg atgaaagctg
     4261 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aggaagtggc
     4321 tgatctcagc caccgagaaa atgacatcaa aaatgccatt aacctgatgt tctggggaat
     4381 ataaatgtca ggctccgtta tacacagcca gtctgcagcc atggtaccgg cagtgtattc
     4441 tgccggacag tctgcacaac ttcctgttgc cattgattat ccggcagctc tggcactccg
     4501 ccagatgtcg atggttcatg atgaactgcc gaaatatctg ctggctcccg aggtgagtgc
     4561 cctgcttcat tatgtcccgg atctgcgccg caagatgctg ctggccactc tgtggaacac
     4621 cggcgcgcgt attaatgaag cactagcgct gacgcggggg gatttttcgc tcacgcctcc
     4681 gtatccgttt gtgcagctgg ccactctgaa gcagcggacc gaaaaagccg ccaggacggc
     4741 aggaagaatg ccagccggtc agcagactca ccggctggtc ccgctctccg actcctggta
     4801 cgtcagccag ctgcagacga tggtggcaac actgaaaatc cccatggaac ggcgtaataa
     4861 acgaacaggc aggacagaga aagcgcggat ctgggaagtg acggacagaa cggtcaggac
     4921 ctggattggg gaggcggttg ccgccgctgc cgctgatgga gtgacgttct ctgtcccgat
     4981 cacgccacat acgttccgcc attcctatgc gatgcatatg ctgtatgccg gtataccgct
     5041 gaaggtcctg cagagtctga tggggcataa gtccatcagc tcaacggagg tctacacgaa
     5101 ggtatttgcg ctggatgtgg ctgcacggca ccgggtgcag tttgcgatgc cggaatctga
     5161 tgcggttgtg atgctgaaac aattatcctg agaaaatgct ctggccagta tatgggaaat
     5221 accccaggtt ttcccgttgg atgggaaaca ggcatgatta ggatgagtcg ccagactgaa
     5281 ccgccccggg aatcctggag actaaactcg ttgagagaga ggtaaacaga atctatgtat
     5341 cgttccggtg aactcgcatt gccgccagac atcatagttg ccattctgca ctttccctgt
     5401 atctgagctg tctcttatac acaagtcccc tcccggggat ttgtgatgtc tgtcagtctg
     5461 ctttagtggg gactctttcc atccggtact ttccttcctc cccggtagcc attttttgaa
     5521 ctttttcttc acccgctgat cttctcttat atttcatttt tttcgggtct gttatggtac
     5581 tttctgattg ttattcatag gctaacgagc agttgtaagc gcccttttta gagcgtagtg
     5641 taagctgggg tttacgctac gctctaaaaa gggtgcttac tctgaaacgg tgcggggaat
     5701 taatacaagg gcattgagat ctgcccggga tgataccgcc agaacccatt gcccagacta
     5761 tgggaaacac accaagtttt ccctggaatg gaaaacaggc ctggttagtg tgtgtcgctg
     5821 ggctggtatg ggaaatagcc caggttcatt attatgggaa acaaacctgg ttagtttttc
     5881 gaatcaacaa tggtgaagac taacctgcca tcttcattct gtgtcattgc ggctttaagt
     5941 ggtgtatttg cgtttatctt tttcagtgcc ggattgataa acgttctctt catttcagcg
     6001 aagtttgatc tttgcgattc aggaagcatg aatcgctctg acagccattc ctgagtagta
     6061 acccatactc cgctggaacg gtactggcac aggctttcat acagtcgaat ggccctaaca
     6121 ctgtttattt ttccgcagtc atagagtgaa tacgtggtga actggctggt cagtcccatc
     6181 agatagggca tgactttgta attaaattca atttgccatt ttccgcgccc tttttttagc
     6241 ccagcctcag ccagccaggg ccgtttgatc tcgtcaaact ctccgtcttt gggataaaac
     6301 acaacactac tttcagccag tgcagtgacg ccctttttaa catcagatga cgctgtatca
     6361 acagacactt tgaaatactt ttgataatca gcaacaagaa ctgtgaatac tggcgatacc
     6421 gaatcaggat catctttcag tggatagcac tgaataaggc ataaccatag caccctcttg
     6481 gcttgcaacg gcaagtagta agctgcttct gtcagttcat tggattgact aatgttgact
     6541 gattttttcg cggtacttat tgtcattgtc attatcgtat gggttgtttt taactcgccc
     6601 atattgtcat gcaaaagccc atccaacaag ggaaattaac gtgttatcca cagtcataaa
     6661 acacgaattt aaaccagggg cgtttcccat agcaatacga ggtgtttccc ataccaactc
     6721 gtggcatttc ccataaaaat tcgggctgtt acccatatta atccgtggta tttcccagca
     6781 taaccctggg tgattcccat aaaggaggcc tgaaaggcag acatgacgcg gtctctgggc
     6841 atcctaaaat ggacaaaaag gagaaaactt ttaaaaagga ataaaaatag cttaaaaaac
     6901 agtctgtgag tatgtggaaa agttgatctg aactgtcaga gaaaactttg ggtgtttccc
     6961 acaatatcct ttaaatcagg tgcgtacgag cattatgtga ctatagcgcc gttgcgcttc
     7021 ctcgccagtt cgctcgcgat gctcggtctc aaggctgcgg cgagcactac ctattccttt
     7081 tggtgataaa tataatgcca cgtacattaa tttaatgtcg tggttataaa tgttgtacgt
     7141 tttgttatgt tgtgtacaat gtgattaagc tttacacata tttgacgcgc aaggaatcca
     7201 tctttagggg aaggggaaaa caatgagacg tgattacggt agtgttggca ccatagctct
     7261 cagagcaagt gccttacttc aggctatgag cagggatatt gaggctcaga gaaaagaatt
     7321 taatctgact caatatcatc agacatatac cagaaatgct gtggcaaaac tgcctaagtt
     7381 aagccgtcgc atcgttgaac ttgctgtaaa agagatggag gacagcggtt acgaattcaa
     7441 taaaaagcag gttggtaacg tagagcaata cgctctcact attcagaacg ttatcgacat
     7501 ttacgcccac cgtcaaatac ctaagtaccg cgatattcac aaagaaccat acgtgatttt
     7561 tgtcgtcaat cttaaaggcg gtgtatcaaa aacagtcacg acagtcactc tcgcacatgc
     7621 attacgagta catccggact tgcttcgtca tgatctccgc atcctggtta ttgaccttga
     7681 tccgcaggca tccagtacta tgtttcttga tcacactcat agcattggta ctgttcttga
     7741 aacagcagcc caggcaatgc tgaatgatct cgatgcagaa acgttgagag aagctgtaat
     7801 tcgcccaact attatcccag gcgtcgatat catccctgcg tcgatcgacg acggctttgt
     7861 tgccagccag tgggagtcat tagttacaga acatttacca ggtctgaaac cttctgaggt
     7921 gctcaaaaaa acaattattg accgtatcgc aggtgattat gattttgtct ttattgatac
     7981 aggccctcac cttgatccgt tcctcctgaa tggactggcc gcgagcgatt tgctcttgac
     8041 ccctactcca ccggcacagg ttgattttca ctccacgttg aaatacctga cacgtttacc
     8101 ggagatgctg gaacgccttg aagaagaagg tgtggaaccc aggctcagcg ccagtattgg
     8161 gtttatgtca aaaatgaccg gcaaacgtga ccacgagacg tcacatagtc tggcgcgtga
     8221 agtgtacgca agtaacattc ttgatgcgtc actgccgcga cttgacggat tcgaacggtg
     8281 tggtgagtcg tttgacacca ttattagtgc aaaccccgtt tcctaccctg gaagtgctga
     8341 agcactgaaa aaagcccgaa ctgaagctga acgatttact aaggctgttt ttgatcgaat
     8401 tgaatatatc cgtggagcat caaaatgagg aaagttttag ctcgtggccg agttcttggc
     8461 aacagcaatt cagaatttgc gcgaatgctt gaaagcgatg gcgatgtgaa aacctttact
     8521 ctcaaatctg gtaaacaggc caaatttgtg aaaacggttg tgctaagcgg tgagatcgaa
     8581 tcgaagacat ttgttgattc atctgttaat ggtcgcgacc aggcaatgtt atcccctgag
     8641 tcagtcagtg acatttcccg taccataaaa ctgcagcagt ttttccccgc tattgggaga
     8701 gaggtcaacg ggcgaattga gatcctggac ggcacacgtc gccgggcagc ctgcatttat
     8761 aacaatgtta aattcgaaat acttgttaca aaggacgata ttgaactgtc agatgcacga
     8821 caactggcaa aagacataca aaccgcacgt gagcatagct tgcgtgagct gggaaaacga
     8881 ctcgaagtta cgtatggaac cagtatgacg aaggaagaga ttgctcaggc agaaaatctc
     8941 tctcctgcga aagtgactcg tgcgtttcag gccgcagcag tgccagatga aatggttgca
     9001 gttttccctg tgataaatga tatttcgttg tcggattatc attttttact gaaactagct
     9061 gaagaagcaa acaacaagca aagatcagta acagaactga tggaaaaagt tcagcatcgg
     9121 ttgaagacca tgccagatta tccggcaatt gataaaagca aaattctggc tgttatcaga
     9181 gctgagagca aatcgctgat agttcgcccg acccgcacgg ttcaaactga gaagctgaga
     9241 gaattttcag atcgcaacca atttgcaaga aagaaaattg attcaaagaa acga
//
LOCUS       DAJOTB010000174         9263 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJOTB010000174 DAJOTB010000000
VERSION     DAJOTB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.174"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            355..1074
                     /locus_tag="OHN28_004195"
     CDS             355..1074
                     /locus_tag="OHN28_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCQ2299485.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1085..2512
                     /locus_tag="OHN28_004196"
     CDS             1085..2512
                     /locus_tag="OHN28_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCQ2299486.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            2505..3200
                     /locus_tag="OHN28_004197"
     CDS             2505..3200
                     /locus_tag="OHN28_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCQ2299487.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(3443..4111)
                     /locus_tag="OHN28_004198"
     CDS             complement(3443..4111)
                     /locus_tag="OHN28_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299488.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(4295..5488)
                     /locus_tag="OHN28_004199"
     CDS             complement(4295..5488)
                     /locus_tag="OHN28_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2299489.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            complement(5510..6586)
                     /locus_tag="OHN28_004200"
                     /pseudo
     CDS             complement(5510..6586)
                     /locus_tag="OHN28_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6628..7389)
                     /gene="hyxA"
                     /locus_tag="OHN28_004201"
     CDS             complement(6628..7389)
                     /gene="hyxA"
                     /locus_tag="OHN28_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HCQ2299490.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYLDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDCYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(7543..8337)
                     /gene="hyxR"
                     /locus_tag="OHN28_004202"
     CDS             complement(7543..8337)
                     /gene="hyxR"
                     /locus_tag="OHN28_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HCQ2299491.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            complement(8667..9230)
                     /gene="ipbA"
                     /locus_tag="OHN28_004203"
                     /gene_synonym="fimX"
     CDS             complement(8667..9230)
                     /gene="ipbA"
                     /locus_tag="OHN28_004203"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HCQ2299492.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDRLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         2467 a         2084 c         2161 g         2551 t
ORIGIN      
        1 gactattact tgatgcgtga ttattgtcgg tgattttttt gtacaccatc agtgtatatc
       61 tcaataaccc ctgaataagt agctctgaat aggtataagg gatgtagcct ttttaaatgg
      121 atttcttatg ccaaaaatga tcgtcgattt ccctataaaa tgtgagcgat gccgaaagaa
      181 ataaaattag ttattgcatt tgaagtttag atgaaagatt atcatttgtc atacaaatga
      241 gaggtggtat gttgctagcc aattaaaaaa gaacgccata tttattgatg attgatcccc
      301 cggaaggagg gaaccatcgg gcataaatgg gcatgaagta atggagtatt agttgtgaat
      361 gtcaatttct ttgtcacctg tattggtgac gccctgaaat caagaatggc acgagactcc
      421 gtgctgctac tggaaaaact cggctgtcgc gtaaatttcc cggagaaaca gggatgctgc
      481 ggtcagcctg cgatcaatag tggttatatc aaagaagcga ttccagggat gaaaaatctg
      541 atcgccgcac tggaggataa cgacgatccc attatttcac cggctggctc ttgcacctat
      601 gccgtaaaaa gttacccgac gtatctggcg gatgaacctg aatgggcatc acgtgccgaa
      661 aaggttgccg cgcgtatgca ggatctcacc tcttttattg ttaatacatt aggggtagtc
      721 gatgtaggtg ccagtttgca agggagagcg gtgtatcacc catcttgtag cctggcccgt
      781 aagctggggg tgaaggacga accacttacg ctgctgaaaa atgtgcgtgg actggagctg
      841 ttgacctttg ctgaacagga tacctgctgc ggatttggcg gcacgttctc ggtcaaaatg
      901 gccgaaatat ccggcgagat ggtgaaagaa aaggttgcgc acctgatgga agtccgccct
      961 gagtatttaa ttggtgctga cgtgagttgc ctgctgaaca tcagtggacg attacaacgg
     1021 gaagggcaga aagtcaaagt gatgcatatt gctgaagtgc tgatgagccg ctgaggatat
     1081 aaagatgtcg atcaaaacca gtaatacaga ttttaagaca cgcatccgtc agcaaattga
     1141 agatccgatc atgcgcaaag cggtggcaaa cgcgcagcag cgtattgggg caaatcggca
     1201 aaaaatggtc gatgaattgg ggcactggga ggagtggcgc gatcgggccg cccagatacg
     1261 tgatcatgtt ctgagtaatc tcgacgctta tctgtaccag ctctcagaaa aagtgacaca
     1321 aaacggcggt cacgtctatt ttgcaaaaac caaagaagac gccacccgct acattttaca
     1381 ggttgcccaa cgcaaaaatg cccggaaggt agtgaaatct aaatcgatgg tgaccgaaga
     1441 gattggtgtc aatcatgtgt tgcaggatgc cgggattcag gtgattgaaa ccgatctggg
     1501 tgaatatatt ctccagctgg atcaagatcc gccctctcat gttgtggtcc cggcaattca
     1561 taaagatcgc catcagatcc gtcgagtgct acacgaacgt ctgggctatg aggggccgga
     1621 aacgcctgaa gcgatgacct tattcattcg gcaaaaaatc cgcgaagatt tcctcagtgc
     1681 tgaaataggt attaccggct gtaatttcgc ggtggcagag accggttcgg tatgcctggt
     1741 gaccaatgaa ggtaatgcgc gaatgtgtac cacgctgcct aaaacgcata ttgcagtgat
     1801 gggaatggag cgtattgctc ccacgtttgc agaggtagat gtattgatca ccatgctggc
     1861 gcgcagtgcc gttggtgcac gtttgacggg atacaacacc tggctgacag gaccacgcga
     1921 agcggggcac gttgatggtc ctgaagagtt tcatctggtt attgtcgata acgggcgttc
     1981 tgaggtgctg gcctctgaat ttcgggatgt gctgcgctgt attcgttgcg gggcttgtat
     2041 gaatacttgt ccggcatatc gccatattgg cggtcatgga tatggctcta tttatccagg
     2101 gccaattggt gcggtgattt ccccgttact tggcggctat aaagatttta aagatttacc
     2161 ctacgcctgc tctttatgca ccgcttgtga cagcgtgtgt ccggtgcgta ttccgctgtc
     2221 aaaactgatt ttgcgtcatc gtcgggtgat ggctgaaaaa gggatcaccg caaaagcaga
     2281 gcaacgggcg ataaaaatgt tcgcttatgc caatagtcat ccaggattgt ggaaagttgg
     2341 gatgatggct ggtgctcatg cggcaagctg gtttatcaat ggcggcaaaa caccactcaa
     2401 atttggcgcg attagcgact ggatggaagc acgcgatctt cctgaagctg acggagagag
     2461 tttccgtagt tggtttaaga aacatcaggc gcaggagaat aagaatggat aatcggagcg
     2521 aatttttgaa taacgttgct caggcactgg gtcgcccgct gcgacttgaa ccgcaagcag
     2581 aagatgcgcc gcttaacaac tatgctaacg agcggcttac ccaacttacc caacaacagc
     2641 gctgtgacgc gtttatccag tttgccagcg atgttatgtt gacgcgctgt gaactgacca
     2701 gcgaggcgaa ggcggcagaa gctgcaatac gtctgtgtaa agagctggga gatcagtcgg
     2761 tcgtgattag cggtgacacg aggctggagg aattggggat tagcgaacgt ttgcagcagg
     2821 aatgcaatgc cgttgtttgg gatccggcga aaggtattga gaacatatca caagcagagc
     2881 aggctatagt gggcgtcgta tatgctgaat atggtttaac cgaatcggga ggcgtggttc
     2941 ttttttccgc cgccgggcgc gggcgttcat tgagcctgct cccggaatct tctcttttta
     3001 tcctgcgtaa aagcacaatc ctgccgcgtg tagcgcaact tgcagaaaaa ttgcatcaga
     3061 aagcgcaggc cggtgaacga atgccgtcat gtattaacat catcagtggt cccagttcaa
     3121 cggcggacat tgagcttatc aaagtcgtcg gggttcatgg tccggtgaaa gcggtgtatc
     3181 tgattattga ggattgctga gagagatatg tagtctggac ataaacctga aggcggtata
     3241 cgaggttgtg aatagcgtta aggacaacct gacttaaccc cgttccgtca tggttatgct
     3301 gctcttctgt aaatatcctg acattaataa tgccaggata tttacagtta aaaagacagg
     3361 aatccattcc tgaagacggg ctcccttccc ggtcactgtc actatagttt tatacaggca
     3421 ctctctgtca gaatgctgtt tattactgcc cagagttttg ttggtattca tctaccattt
     3481 ttttgacaag gcaaaacatt acagaaatca caatacttag aaaaaatccc agagccaggc
     3541 aaatggcagc agggtattta ttttttattt ctggtgagct tatagtgaca cagccatcac
     3601 cgttcggata cagtgaccag ttcctggtca gatatatcat ttttgtataa agctcactgt
     3661 taattgctga aggcgtttgt atctcatcga gaacttgcct gccattatgg tattctgacg
     3721 tataccattt aatgatagac gcctgcacgc gagatatttc cgcttcagca gttggtaaat
     3781 ctctcttatc tcctgaccat tccagcttta tttctctgtt cttgacatct gggctggtct
     3841 gaaaaaatgt agtaattcgc tgaataacat cttcagtcat tgtggtctga caggcaaagt
     3901 tattattgct atcacagcta acaagtatgc ctggtgttgt ctgatttatt tttaggctgt
     3961 aactcacttt tatattatct ttattgatat acagaaacaa cagcaccatt gccatacaga
     4021 tagctataat aaaaaggata acacgaatct ttttcttcag ataaaataaa atctcaatca
     4081 gatcgatatc ctgtttgatt tgttcattca taatatatcc agaaaatgaa atctgtcgca
     4141 gataaggttg tattaatagt ctgtatcagg aatgttcagg gtaaatatca gcaaaaagcc
     4201 agcatcatga gtactggata tttatagcct gcaggcagca tactgctgcc tgcagggggt
     4261 ataacagtcg acaggggaac cgactgcttt attcttacca ggtcacattg ataccaacta
     4321 caccctgtag cgggttctca atgtcgtcac ctttggtgta gtcaaggctt gcatatgctc
     4381 ccatgttttt ggtgatatca gcctgtacac ctgcacccac gcggaatgct gcaccgtcaa
     4441 tgctggcatt gacagactca tcacccagac ggactttgtt gccatcagag aattcgttca
     4501 gtgcggccag attcagataa ggtttaacgg tcgcgttgcc cacagcgaag tcataaccca
     4561 cgcgcgtacc ggcctcaccc agcacagagt tatagctgtc agaatgaacg tccacgccgt
     4621 tgagttgata atccacacct tccagagtca gagcagacag tttggcatat ggctgaataa
     4681 acaggccatt accgtctttc catgtgtaac cgcctttaac catcgcacca aaaccattgg
     4741 tgttaaagtc agctttgatg gtaccgccac cattcatcag gcgaacatct gccgtgttgc
     4801 tgtagtgacc aaactgtgca gcagtatcaa tgaagatacc gttgttatac tggcggctca
     4861 ggtaagcgtg gaagctgtaa ccttcagtgt cacctttgct ctgcatggta gtcatgtcac
     4921 ctttcgcaga agacatcgcc acaccggcca gccagctacc atcttcagtc atgaagcggg
     4981 tatcaccacc cagcattaca ccatttacat ccaggtcata ggatgcattt cccgcggtgg
     5041 tatgtttctg tttaccgcca aagtactgaa tccatacgcc acccttgtca ttttcgctca
     5101 gacggacagc atcctgacgg gcggagagcg tgtcagcctg cagattagcg acgttagccg
     5161 gagcagctgc cattgcctga atcacggagc tggcgttgga tgctttatgc gtgtttttta
     5221 cgaaataaaa gttaccatca gctccctgtg tcaggtcata ttgatacaga ccaccttccg
     5281 ttgtgcctgt ggcttttaga gtgctgtctt tgccggtaga aataaatgcc gtttgttcat
     5341 tgataagatc caatgaaccc gcactgtaaa cactcaagtc accgctcgct tttccgctga
     5401 tatccagagt gttttcagaa gaggcatcaa caatcagagt gccgttttga gaagcaaacg
     5461 tgccagtatt gactgtagcc ttcgtgatat taaccgtact gtcttttact gccagagcat
     5521 caacagttga ggaacccgta acattccaga cagacccgtt ttgcagagat acactggcat
     5581 ctcctgcccc tgaaactttg cctttccaga ctgaactatc ggaaagattt atatccagat
     5641 cactacccgc tttatcgccg ctattgagcg tataagcatt accttcccag taagagcggg
     5701 caacgttcat atttacattt gttgtggtac cggctgaatt ctcgctttca gaaagggcca
     5761 ggacatcaga gtagatggtt gaatcagcaa cagacaaatt taaattttgc gttgctgaac
     5821 ctgcgccttg tgccgaagca gaggcataaa ttgtttgttt attattagcc gccccaatta
     5881 ttgagccatc cttaatattc aggttgactg tatgaattcc cttattctcc ttggcaataa
     5941 ctattgtttc aatcccacca ttaataatat ttgagtcagc aagggtgatg ttgtcggttg
     6001 catcaccgtc aaaaccctgc aacaaattac ccttttgagc aaaaattgcc gtagagccgt
     6061 tgctggaact cttattacct tgagcattta tggttgtgcc attgagcgtt aaattatttc
     6121 ccgtggtcgc ttccagagat atacctgtcg cactactttt ttcatttttt gcaacaaagt
     6181 taccgccatt aacaacaaca tcgccagttg aatcaatgac cattgcagtt cggacatagg
     6241 taccttcacc ttgagctgtg atggtggagc ctgtagcatt aatcacaaca tttttgtcac
     6301 cgctaacttt tacaccttca acgaaacctt ggtctaccgt tgatgtggcg gtaatatcat
     6361 gaccacccaa agtaacatta aggtctttat ctttcgcatt aattgttgcg gctgtaatct
     6421 gtgtagtacc actaataaca acatcttgtt ccagagttgt cttatcttta tccaatgtaa
     6481 ccggtgtagt tgataccgcg ttatcagcag cgaatgctgg tgcagaaaga gccataacaa
     6541 tagctgtcgc taatgcgctg cggtaaaatg ccttactgtt tttcatgaag aaattctcca
     6601 ttctggtaac taatttcaat ttcccactta tttatattta tgcagagata aagcctgcgt
     6661 ccctgcattt accaccacgg aatacctgtt ggtatttccg ttttcacgca atgtcatggc
     6721 attcgttgtt tccaatgcat ttccatcacc gtgaaaatta ttgctgctca gcagcagggc
     6781 ttcccgcaat gacatgctgt ccggtatccc ccaacgcttt tttagcgact gctcggcaag
     6841 ccatatctgt cttatccccg ttttgagaaa ctgcgcactg tattcccagc tgtgtccggc
     6901 aagaagcaac gacaacacct tcttttgctt ttccgggaag cgttctgaca tgacgattgt
     6961 ctttgtgcgg ttattcacct ctttcataaa gacagagtaa cagtccataa ctggcaaatt
     7021 ttctgcatgt ttcagtcctg aaaacccacc agaggcattt gcaatactga tgacacgctc
     7081 tactgacatt cctggtttca gtaacctcag gtttttgaga acgccagaaa tactcacatc
     7141 cggtggcaga aaaaagggcg cgacgcacaa caccaccacg aatgagtgat ccagataaaa
     7201 tcggaaccac atttcagcgc attcggcagg tcgccctgcc gatatcacga cactctgtgg
     7261 cctgtgccgc aatttattgc gtaactcctc ttctgttctt atgcaggtaa cattgccgac
     7321 agaaccggtt gatagagctg cgaactgcgc tggtacaatt cctgagggac agaaaagaac
     7381 gaaacgcatc cttccctcac agatgtcagt acgccagcac gctgtacagg ctgtacagca
     7441 tgcctcccaa tcctgcagcc cagaacagta gaaatatttt ggctcctaca ctgtagcgag
     7501 tccgtgtgcg ccgcagtggg tggtagtaac aacgctgctg tgtcataaca atcctggtct
     7561 ttttttagat atattttttc tttgtccgga aagtactttt ccggaaatgg cgtcagaatt
     7621 tattgattca ccaacggcaa ctttctctgg ataaaggatt ccggatacca tgttctgcac
     7681 atactgagtg agtcgatgag cggaatgttg tccgatatat tttgcatgta gccgtatatt
     7741 ccgtttcact ctgctgtcac cgcatcccgt atagagcagc ctgaaatcgc tcagtgttct
     7801 cagtccaagt ttcattaaag cccgtgtggc aatcgcatgc agggtctttg aactgcgttg
     7861 ttcataacgg gcaatctcac tgaaactcat gccctgcatc agaatcttaa gcacgctcaa
     7921 ctcccgtgca gtcaggagcc agaaatcgct catatcgccg ggcaaaggac gcagtaacca
     7981 gcggataatt tgattctgat aacgactcgc tgtcagcatg gtgccgatgc gtgggaagcc
     8041 gttatacaga cttgcagcag gagcattatc gaaccagtcg gagtcaccgg tcaggcagac
     8101 ctgccattgc ccggcatcac tccgcaaacg ggcgagacaa gccagaagaa ccaactcccc
     8161 cgaaggaagc gaagggatat cagctaacag gtagccacag gaggtcttta aaacgaattc
     8221 aacctctgta gtgacgttgc tgtacacccg cacgatatac ctgtccgttg ggatgagaga
     8281 ctccagtgcc agcctggtga actgacaggc actgataata accagaggct tcttcattca
     8341 gactcgtcca cgccattgct acaaaacctg acattttgat tcaaaatgtc aggtttggtt
     8401 taatagcctg aatatttaat tttcttggaa tataaatcta atttgtttga tttttattgt
     8461 ttttaatttt gtgttattta acgatatggg cgaaagttaa tcactggtat gacctttgtg
     8521 attaatcatt tcaaatcata gggttatttt actttagcta gaaatatcaa catgttatgt
     8581 gttttattta attgaatggg ctgttttttt atggaagaat tgaggaaaac gtaggaatct
     8641 gacattttga atcagaaggt actgttttaa cgaggctttt ttttccacac ccctttaaaa
     8701 cgagcagcat tactggctgt gtatctgaca gtatgctgaa tatttcgatg acccagataa
     8761 tcctgaagta gtcgggtatc gacaccattg tcagccagag caaaaccaca ggcatgacgt
     8821 aacatatgag gatgtgcttt tacagctaat cctgcacgtt ttccagcctc acgaatgata
     8881 gaaaaaaaat gttgtctgct gatagggcgg cgtttccggg agagaaaaag cctgtctcct
     8941 tcaactccgt tttcaattaa cttcctctgc ttaagccaca acttgatcaa attatattca
     9001 tcaggaagaa gaggatgtgt tgtcgagaaa ccatttttaa tacgccgaat attaagttgt
     9061 tttccagatg catcaatatc ggataaacgt aaatcaagaa gttcactggc cctaaaaccg
     9121 tgaatgaaag ccatcataat tagacagcga tttctttcag gaaaggacat gctctgggct
     9181 gcatccatca gcctgtagac ttcatcctgt gtgagatatt ttctcgtcat acgccaccgt
     9241 gtacagttgt aaaaaaaaga gag
//
LOCUS       DAJOTB010000175         9206 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJOTB010000175 DAJOTB010000000
VERSION     DAJOTB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.175"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..268)
                     /locus_tag="OHN28_004204"
     CDS             complement(<1..268)
                     /locus_tag="OHN28_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCQ2299493.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVL"
     gene            complement(349..1200)
                     /locus_tag="OHN28_004205"
     CDS             complement(349..1200)
                     /locus_tag="OHN28_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCQ2299494.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(1212..2303)
                     /locus_tag="OHN28_004206"
     CDS             complement(1212..2303)
                     /locus_tag="OHN28_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCQ2299495.1"
                     /translation="MKENKITVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(2328..2642)
                     /locus_tag="OHN28_004207"
     CDS             complement(2328..2642)
                     /locus_tag="OHN28_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCQ2299496.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(2660..3130)
                     /locus_tag="OHN28_004208"
     CDS             complement(2660..3130)
                     /locus_tag="OHN28_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2299497.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(3157..4710)
                     /locus_tag="OHN28_004209"
     CDS             complement(3157..4710)
                     /locus_tag="OHN28_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCQ2299498.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVDYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(5005..7323)
                     /gene="yicI"
                     /locus_tag="OHN28_004210"
     CDS             complement(5005..7323)
                     /gene="yicI"
                     /locus_tag="OHN28_004210"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCQ2299499.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(7333..8715)
                     /locus_tag="OHN28_004211"
     CDS             complement(7333..8715)
                     /locus_tag="OHN28_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCQ2299500.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            9008..9102
                     /locus_tag="OHN28_004212"
     tRNA            9008..9102
                     /locus_tag="OHN28_004212"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9042..9044,aa:Sec,seq:tca)
BASE COUNT         2490 a         2402 c         2040 g         2274 t
ORIGIN      
        1 gcaaaacatg ctctacagag gcaccatgat cgaggtgcag tacaaacggg accggactac
       61 gctgggtaat ttcgcgaacg tgagcgaaaa aggcatcgcc cacaaaatca tgttcgctgg
      121 gatggatggc aataatcgcc ggagtatttg tcgcttctgc ttcatccacg actgcgcgaa
      181 ggaaacagtt atccgccaca ttaaatgcgc caatggcaaa gccgtgctgg cgggtaggtt
      241 gcagtaattc tttcatcgaa actaacatgt taaatctcca gtttcagcgt gtagtccgcc
      301 ctgccggtgc gttattgcac tggcataaag gccatgacag aaaatgaatc aggctttgcc
      361 gatagcgccg ctcgcctgta aatacggtgt aatcgcttct tcgaaggcca gttccatagc
      421 tttcagcgcg tccaccagtt caatggatat atcgtgacag agggtatgca ggagcgcgga
      481 tttcccccgt tcaaaaatgg ccgcccccac gttgattttt gctaccccca gagtcgtaca
      541 gagctggagc tgatcgaacg gcgtaccgct accgccatgt aatgccagag gcacgctcga
      601 gcagtggaaa atctccgcca gacgctgaaa atcaatagca ggtacaacgc caggcgcgta
      661 catgccgtgc gccgtgccta ccgagacggc cagtagatca atgcctgtct gcgcgataaa
      721 cggttccaca tcagtgggtt gcgtcatcag gtctgcattg gcggcctggt cgtaacgcgg
      781 cgcatctgcc agatggccca gttcgccttc cacgcagagc tgcgcgctgg cggcgagttc
      841 caccaccgcc ctggtctggc gcacgttttc ctccagggga aaagcagaag catcaatcat
      901 taatccgctg aaaccagcac gaaacgcctg acgaatacag tccggatttt tgccatgatc
      961 aagcgccaga gcgaccggta ctgaggcaga ttccgcgagc gttttaacca ctgccgccgc
     1021 gacgggtgca ggaaaatgcg ccagatgccc ctgatagaga ttgagaatga tcggagcgcg
     1081 attcttctcc gccacgcgga tagctgcgtg agcactttcc agattcatac agttgatcgc
     1141 cagcaaggcg taatgttcat gccaggcttt catcaccata cttttcatat cggcaaacat
     1201 agggcctccg gttattcgag ggtgaaagtg atctcttcat ctttcagctt ttgatcgctc
     1261 agttcgtcaa actcttcgtc gcgttccgtt accggtttct tccagagtga aagcatcacg
     1321 ccacaaatca ccgtgcctat acccagcgcc aggcaaaaca gcagaggatg ggtgaacaga
     1381 ggcacaacaa acatgccgcc atgcggaacc ggactctcca cgccccacac catgattaag
     1441 ccgcccgcga ccgccgatgc caccacacaa gatcccacta ctcgcagtag gtcacgcgcg
     1501 gcgatcggaa tgactccttc agtaatcatg cagatcccca tcggaaacgc ggctttcagc
     1561 gcctcgcgct ccgcacgggt gtatttatgg cgtgtcagta agaacgaaag tgtgatgcca
     1621 aacggaggga tgattgagcc gacaaatttc acggcttctg gtccgtagac gccactgacc
     1681 agcaagccat cggaaaacag cgacatggtt ttgttgaccg gaccaccgaa atcgaaggtt
     1741 gccatcgcgc caagtatggc ccccatcagg aagcgcgatc cgccctgcat cgactcgagc
     1801 aaatgaatca gtgcttcttg taaccaggcg atgggtttac caatcagcgt catcattaac
     1861 agcccaccga taatggtgct taataccggc aacaccatga ttggcatgag tccttgcatc
     1921 gactgcggca ggcggatata gcgtttaagt agcaaaatgg tgtagcccac caggaaacca
     1981 cccaacatac cgccaataaa tccggtatgg atttgcccac agagaaagcc gacaatcagg
     2041 cccggggcaa atccgggacg atcggcaata gaataggcaa ttgccgcgct aatcagcggc
     2101 acaatcagcc ccattcccca gccgccaatc tgattaagca tccacgggat tgttcctgtt
     2161 ttttcaccaa catctgtacc gccaagaact tgccccaacg caatacatat cccggcggcc
     2221 acaataaggg gtatcatcca cgagatgccg gttaataaat gttttttaat ttcctgtgaa
     2281 accgtaattt tattttcttt catattaaga tccctccagt ataaggatca tttgtttccc
     2341 agtgattttt cgaccttttc aagaaattga acgggcgact taatgacgat ctctgtcttt
     2401 actcgcacaa tacgtttatt ggtgaagcgt tcttctcctt ttattttcac atctacagca
     2461 agaatcacga cgtcagcagc agagatatct gccgcctgta attcattttc tgtaccaatg
     2521 gttccctggg tttcaacttt aatggtatgg cccagagctt ttgctccttt gattaacttt
     2581 tcacgagcaa tataggtatg agcaataccc gccgtacagg cggcaacaca gacaatattc
     2641 atgacatact ccctgttatt tagcagacat cggattgtgc atactgacta aagagatcaa
     2701 tgattttttg cggactcgtc tcaactaaca attgttctat gatgtcatca tccgccagcg
     2761 cgcccgcgac ctgggaaagt aaacggatat gggtggtatt ttgatcttca agacgtaccg
     2821 caaataaaat aatacagcgc acgctactgc catccagcgt ttcccaggga atatcctggc
     2881 gggtacggcc aattgccagc gtagtattaa tcacagctga agatttacca tgcgggatcg
     2941 caatatgatt ttcaaaacca gtcgagcctt ccgcttcgcg ctgccagaca tctttaataa
     3001 aggtttcccg acaatcgatc gcaccatctt ttttgagaag attagttaat tcttcaatcg
     3061 cctccgcttt attcgtcgct tgcatatcta aaataacgcg attcgtattc aggatttttg
     3121 ttatgtccat tcgtcatacc tcataaaatt ctgacattaa atttcaacga cccgagacgt
     3181 cgcatgatgt aatttttccg taatcaccat ttcagtgacc cgcttaatat cggcatcatt
     3241 aaaaaaagcg gtaacataag cgaccggcat agtgactgta ggcaaattaa tggtcgaaat
     3301 aatcagttcg atattgtcag gcaaaacctg tgataaattc gctgctgaaa taacatcaac
     3361 aatcgtccat tcaggaaatg ctcgcagaat acggcttttc agtagatgcg aagttccgat
     3421 tccggttgaa cagaccagta atacgcgttt acgggcaatt tgccgttcca tcgcagcctg
     3481 aaaatgaacg gtcaggtaat ccacttcatc ctcgctcacc gcattctcac cccaggtttt
     3541 aaatacctga ttcaccacat attgcgtcaa ccgccagaca tccgctaatt ctgctttaat
     3601 atcttccagc aatggattac ggatatgaat acgataattt agtcggttaa tcagcggttt
     3661 aatatgcacc aataagccat catataatgc gctgtcttca ctaaaatcgc agtccacgat
     3721 gtcactgaat accgtaatta aacgccaggt aatcagacgc gcttcattgc tggcctgcat
     3781 atgtgaaata atgctaacat ctttttgtcc atcaatcgcc acacctgatg aaatgatata
     3841 ttgataaata aaccagactt catcatcggg caatgtatgt gcaattcgtt gttctatctt
     3901 atgaatcatc tgactggcaa cattaaagat attttcatca tattgcctgg tttgatcttc
     3961 ttctcttgat aacgcattcc cgtgcgtgtt gcgatacatc ataataagga tatgagtaaa
     4021 aatgttaacg taataaggtt ctcccaaaga ccaacttaat tcattttcca tatccagtaa
     4081 caatgactgg acaaataaca cttcttcctc tccaaaataa tggactaatg ctttatagct
     4141 tccgggatcc agacgtgaat acacgacacc ttgcggctca ttatggttaa taatgccgtt
     4201 aatcagtaat gccattgcct gtcggacttg cccttcacta ccttcaatat gcgtaccact
     4261 tgggctgcgg atcaacgata accccaacgg cgcaagccag gactcaatta ctctcaggtc
     4321 attaacaata gaggctccgc tgataaaata gcgttctgac aatttactga tcgacgtttc
     4381 attcggtgtt tcacttaata actgcgacgc tattttaacg cgcctggcat tattcattac
     4441 accatcaatc tcttcacatt ctgccaccaa caaatgatca agatgcagca aatcagcaat
     4501 attctccgcg cttaacatca cacccgcgcc agcacgcttc tcaacattta ttctccattg
     4561 tcccagccat tgctcaagta aacgtaaatc acgttgaatg gttttttccg aaacatttaa
     4621 atagccggca atcgtaacaa tagtcgtata ttcccttcga ggtaaaagga atcgtaataa
     4681 tctgttttgt cgcgaggtaa tcatttgcat tttttaccct tatcacagat taagaagccc
     4741 catatggaag agcttcagtt gccatctctg agggtaaata taaagcagaa taatttcaca
     4801 acaatactaa aatctgtccg taatccggac agtgataaaa caaagcaagg cggttaaggg
     4861 atatttttca gcacagatca cattttcgtg atgaataatc gtgatcatgc tcgcaaatac
     4921 gatggaagaa atatttatta ccagatgtga gattatttac cggatacgac acaaaaccat
     4981 tcgtatccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc cttgaggagt
     5041 caccaccagt ccctgctcac tttcagcctg cgaaccgcct tgcagaccat ttactttcac
     5101 aatattgcgc aagcacagtg tccagttctt cgcctcgccc gtaccagtca cagtaatcgt
     5161 gttgccagta cgtgctgctt ttaaagtaaa gatcaccgaa ccgtcagcag tgggcacttc
     5221 acagacggct tcatgcccgt cttgcagatt aaagaggtgg aatgccgtac cttcgtgcca
     5281 cgcgtaatcg ggacgttgat cgttgttgcc cagcgccagt agggtgctat cacgcacata
     5341 aacgggcaga ctcaggaagc tgtgctgctg tttatgccag cgactaccgt cgagttcatc
     5401 gttgtgccac aggtgtgtcc agcgaccttc cggcaggtag aactgcacat cgcccgcttc
     5461 agtgaacaca ggcgcaacca tcacgttgtc gcctaacatg tattgacggt caaggtaatc
     5521 acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg taccccgggc
     5581 gtttgcacgt gcagcttcac gatacagata cggcatcatg cggcatttca gttgcgtgaa
     5641 gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat aagatttgct
     5701 gccatgtaaa cggctatggc tggagagcaa accaaacgcg caccagcgtt tgtaaacgtg
     5761 cgccggagcg gtattttcaa agccgccgat atcgtggctc cagaagccaa aacctgaaag
     5821 gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag cgtaacagtc
     5881 gccaccccag tgtaccggaa atttctgcgc accaacggag gccgagcggg caaacaagac
     5941 ggcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt tgtagatgta
     6001 tgcataatgg ttatgcattt tctgcggatc ggaaccatca aaccattgaa catcggttgg
     6061 aatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca gacctttcag
     6121 tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg ccagacctgg
     6181 ctgccattta tcccactgcc acagcgaacc gtccgggcgt ttgagtaaat aacctttctc
     6241 ttgtaactct ttaaagacgg gggatttttg cccaatatag gggttaatcc agacgcagat
     6301 tttcagccct ttcgctttca ggcggcggat catcccttcc gggtccggaa aagtcagcgg
     6361 atcccactca aaatcgcacc actggaaggc tttcatccag aaacagtcaa agtggaaaac
     6421 atgcagcggc agattgcgtt ccgccatacc atcgataaag ctgtttaccg tcgcttcgtc
     6481 gtagttggtg gtaaatgaag tggttagcca cagaccgaag gaccacgcgg gcggcagcgc
     6541 cggacggcca gtaaaacggg tataacgatc aagtaccgct ttaggcgtcg ggccgtcgat
     6601 aacaaagtat tcgagatatt cactctcaac gctgaactgt actttggaga ctttctccga
     6661 tcccacttca aaagagacac attggggatg attgaccagt accccataac cacggttagt
     6721 cacataaaac gggatatttt tatacgcctg ttcagtactt gtgccgccgt cccggttcca
     6781 ggtttctacc gtctgaccat tgcgcaccag ggcagtaaag cgctctccca ggccgtaaac
     6841 tgtttcgcca acgccaagat ccagccgttc aaacatataa tttcgttgat tattcgtgtc
     6901 ctgcacgtag ccattatttt tcacctgact accggtaata cgttcgccgt tgcgcagaaa
     6961 atccagtgac cagaactcac ctttgctgac acgcgcactt aagttgccac ttttaaactc
     7021 agcgtaacgt tctgtgtttt cgatagtgac cttcacgtcc tgcaaaatat tgagcgggta
     7081 atgaggaccg ttattcagcg ccccctgaaa atgctcaatc cgtacaccga caataccttc
     7141 ctgtggggag aaaaagcgca gcgtaaataa aggcgtatca agctgccagg tacgttcacg
     7201 cacatcacga ggggcagcat agaccaccat ttcattaccc tgctgttcaa cctcgaacac
     7261 ctgaagcggg tgaatcaaat tgaggccagg ttgaatcaac cagtttccgt cgctaatttt
     7321 cattgccgtt ccttagttct gcacttcttg agaggtgaat tgttgctggc aacggcgttt
     7381 gccctgcgcc agctgttcca taacggtttt cagattgtgc gtcgtgagcg agtagtagcg
     7441 tttagcgata atcgcgctca gcaagtaaca gatcgccgga acaatcgtga atagcgcgat
     7501 aatgatgcta attgtggcgc tgttctgcgc tttttctgcc gcatcatatc cgccataagc
     7561 cagcatccag ccgataaggg caccaccgaa ggccaacccc agtttgagca caaacagcgt
     7621 gccagcaaaa ctgatcccgg tcaggcgttt accattacac cactcgccgt agtcgacggt
     7681 atcggacatc attacccact ggataggtgt caccagttga tgcaacacac caatcacgaa
     7741 gatgaagaca aacatggtga tgctggcctg cataggaaca aagaacatcg cgaggctaat
     7801 cactgccagc agggcgttcg tccaccagaa gatagtgact ttacatttcc agtcggtcag
     7861 cggttttgcc agtgcggaac caatcaggtt acccacgcaa taagtggtga gaaaagcaac
     7921 aaatacttcc ggcgtaccca aaatccatgt gacgtaatac atcatcgccc caccacgtac
     7981 gcacaccgcc aggatattga aaatggtcag gaaaccgaca atacgccact ggtcgttttg
     8041 ccagatatca cgtaaatcat cccgcataga cgtcgttgta ggtggtgctt caacgcgttc
     8101 tttagtggtg aagaaacaga atgccagcat cataaatgcc accacggaaa ggaccgcgat
     8161 accgccctgg aaaccgagtg gtttattatc accaccaatt aaattaacca gtggcatcat
     8221 cagaacagta gagagcatgc ctcccgcggt cgccagcaca aaacgccagg attgcagcga
     8281 gatacgctga gtcgggtcat tggtgattac accacccaat gcgcagtaag ggatattgac
     8341 gatggtataa agtaaggtaa gtagggtgta agtaattgct gcataaatca tttttccgtt
     8401 catactgaga tctggcgtgc tataggctag tacacagacg atcccgaatg gcagtgcacc
     8461 aaacagtacc cacggccgaa atttacccca gcgagagcgc gttcgatcgg ccaacaatcc
     8521 catgcaagga tcggaaatcg catccagtgc acgagcgacc aaaaacatgg ttccgacaaa
     8581 tccggcagga atgccaaaaa tatcggtata aaagaacatc atatataaca ttacgttatc
     8641 gaaaataatg tggctggcgg cgtctcccat gccataacca attttctctt taacggacaa
     8701 cacttcactc ttcatctgct tttccttgat gctatagggg tgctgagacc gataacgcat
     8761 tttgtaaacc atccctgcga gagcgaatat tccgatatct ggttattaat ttatgattct
     8821 tgttttatgt gatcgtggta gcgttaattt cgctcatata tcattgtaaa atatgagttt
     8881 tatatgaact ataatgcttt cgtgataata cgctgcgtgt attaggcgga aaaaactgat
     8941 ctgggggatg tagaaactca aggaagtagc tataatgcgc cccgcctcca tgtagcaatc
     9001 gaggcgcgga agatcgtcgt ctccggtgag gcggctggac ttcaaatcca gttggggccg
     9061 ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc caaacttcat ccagccagtt
     9121 ccgagatgct ccataaaacc ctttatattc aatattatga ataatcatct tatccgtaac
     9181 agtccgagat cgtctcccta aatccg
//
LOCUS       DAJOTB010000176         9098 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJOTB010000176 DAJOTB010000000
VERSION     DAJOTB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.176"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(151..4038)
                     /gene="purL"
                     /locus_tag="OHN28_004213"
                     /gene_synonym="purI"
     CDS             complement(151..4038)
                     /gene="purL"
                     /locus_tag="OHN28_004213"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCQ2299501.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4296..5852
                     /gene="mltF"
                     /locus_tag="OHN28_004214"
     CDS             4296..5852
                     /gene="mltF"
                     /locus_tag="OHN28_004214"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCQ2299502.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5849..6385)
                     /gene="tadA"
                     /locus_tag="OHN28_004215"
     CDS             complement(5849..6385)
                     /gene="tadA"
                     /locus_tag="OHN28_004215"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCQ2299503.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6410..7045)
                     /gene="yfhb"
                     /locus_tag="OHN28_004216"
     CDS             complement(6410..7045)
                     /gene="yfhb"
                     /locus_tag="OHN28_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCQ2299504.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7254..8102
                     /locus_tag="OHN28_004217"
     CDS             7254..8102
                     /locus_tag="OHN28_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCQ2299505.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            8158..8418
                     /gene="yfhL"
                     /locus_tag="OHN28_004218"
     CDS             8158..8418
                     /gene="yfhL"
                     /locus_tag="OHN28_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCQ2299506.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(8612..8692)
                     /gene="shoB"
                     /locus_tag="OHN28_004219"
     CDS             complement(8612..8692)
                     /gene="shoB"
                     /locus_tag="OHN28_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCQ2299507.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
BASE COUNT         2244 a         2461 c         2289 g         2104 t
ORIGIN      
        1 accaaaaagc gacacggcaa accacccatt gtcgtgattt aaagacacaa aaaagctccc
       61 gaagttggga gctcataata gcggttggtg cttaatgccg gatgcggcgt gaatgcctca
      121 tccgggctgc aataccaatg ggttgacgac ttaccccaac tgcttacgtg cattgcggaa
      181 aatgcgcatc catgggccat cctcgcccca gttttccgga tgccaggagt tgctgacagt
      241 acggaaaact cgttccgggt gcggcatcat aatggtgact cgaccacttt cagtcgtgac
      301 cgccgtaata ccgttcggtg aaccgttcgg gttagccggg taggtttcag tgactttgcc
      361 gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg ccagatgcgc
      421 cgcatcacgc acttccacgc gcccttcacc atgagacaca gcaatcggca tctgcgagcc
      481 caccatcccc tgcaacagca gcgacgggct ttgggttact tctaccagac tgaaacgcgc
      541 ttcaaagcga tcggaggtgt tacgcacaaa acgtggccac aactcgctac ccgggatcag
      601 ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca gcgtttgcgg
      661 acgatggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga tagacttcgc
      721 ccaaccttca ccagcgccca gcacatcacc gtaggagaaa ccaccacacg cgaccagggc
      781 gtggaaaccc tccaggcccg tgcgtccagc cagcagatcg ctcatatgca cgtcgatagc
      841 atcgaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca cgccctgctc
      901 acgcagcacg gcaactttcg ggcgcacgcc agtggcaata aacggtgctg ccacatcttc
      961 gttgatatca aacgacagtt tcacattcag gcccggatcg gcgtcgtttg atttcgcctg
     1021 gtgttcctga tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc
     1081 tgcccaccag acacgcagcg tggtgcggct ttcgctaaat acagtctgcc cgttggcagt
     1141 aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat cagcaagccc
     1201 atgctgtgcc agtaccgctt caacggcttc acgatcagca gctcgaacct gaatcaccgc
     1261 acccagttct tcgttaaaca acgccgccag gcgatcgtca cccagagagg cgatatccgc
     1321 gtcaatgcca caatgaccag caaaggccat ttccgccagc gttaccagca ggccgccatc
     1381 agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata
     1441 gaagcctttc agttgggcaa catcgcgtac atctgccggt ttgtcgccaa gctgacgata
     1501 aacctgcgcc agtgccgttg cgcccagcgc gttattgcct ttgcccaaat caatcagcaa
     1561 cagtgcgtta tcttcggtag aaagctgcgg cgtaatggtg tgacgtacat cttccacgcg
     1621 ggcaaatgcg gaaatcacca gcgacaacgg cgacgtcatt tcgcgctctt cgttaccttc
     1681 ctgccagcgg gttttcatcg acatagagtc tttacccacc ggtatcgtca ggcccagcgc
     1741 cggacaaagt tcttcgccca cagctttaac ggcttcatac aggcccgcat cttcaccagg
     1801 gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat cgccaatttg
     1861 tgttgcggcg atgttggtta gcgcttcacc gactgccaga cgggcagaag cggcgaaatc
     1921 cagcagcgca actggcgcac gttcgccaat tgccatcgct tcgccatagt agctgtcgag
     1981 gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggcccca ccatctgatc
     2041 gcgcgccacc atgccggtta cgctgcggtc accgatggtc accaggaaag ttttctccgc
     2101 cacggtcggc agatgcagca cgcgtttcac cgcgtcagcg atggtgatcc cttcacgggc
     2161 cagcgcgtcg cctttcgctt tcagcgtttg cacatcgcgg gtcatcttcg gcgttttgcc
     2221 aagcagtacg tccagcggta gatcaatcgg ctgattatca aaatgacgat cgtgcagaga
     2281 aagatgcagt tcttcggtcg cttcaccaat caccgcgtac ggtgcgcgtt cacgcttaca
     2341 cagttcgtca aacagcggta actgatcggc agcaactgcc agcacgtagc gttcctggga
     2401 ttcgttacac cagatttcca gcgggctcat gcctggctcg tcgctcagaa tatcgcgcag
     2461 ttcgaattta ccgccgcgcc cgccgtcgct caccagttcc ggcatggcgt tagaaagacc
     2521 gcctgcgcca acgtcgtgga taaacaggat tgggttggca tcaccaagct gccagcaacg
     2581 gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta cggaagcaaa
     2641 gtcgaggtcg gcatcagact gaccagatgc catggaagac gccgcgccgc cgccaagacc
     2701 gatgttcatt gccgggccgc cgagaacgac cagcttcgca ccaacgttga tctcgtcttt
     2761 ttgtacgtga tcggcgcgaa tgttgccgat cccacccgcc agcatgatcg gtttgtgata
     2821 accgcgcagc tcttcgccat tgtggctgtt cactttttct tcataagtac ggaagtagcc
     2881 gttcagtgcc gggcgaccaa attcgttgtt aaacgccgcg ccgcccagcg ggccttcggt
     2941 catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt cccacggctg
     3001 ttcaaagccc ggaatacgca agttggatac ggagaaacca accagaccgg ctttcggctt
     3061 tgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccagaaccgg tcgccgcacc
     3121 cggccacgga gaaatcgccg tcgggtggtt gtgggtttca actttcatca gaatatgcgc
     3181 cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc gacccacttc
     3241 agaaccttcc attacagcgg cgttatcttt ataagcagag agaacgtggt ctggcgtggt
     3301 ttcgaaggtg tttttgatca tcttgaacag cgatttcggc tgctgttcac cgtcgataat
     3361 ccagtcggcg ttaaaaattt tgtgacggca gtgctcggag ttcgcctgcg cgaacatata
     3421 cagctcgatg tcgttcgggt tacgacccag ctttgtgaac gcatcctgca gatagtcaat
     3481 ttcatcttcc gccagtgcca ggccaagacg caggttagcg tcgattagcg cctgacggcc
     3541 ctgccccagc aaatctacgc tggtgaccgg agtcggttga tggtgtgcaa acaactgctc
     3601 tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt cagcggtcac
     3661 ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcca tgccgcgctc
     3721 aaggcggttt acctgttgta gaccgcaatt atgggcgata tcggtcgctt tcgaagacca
     3781 gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg gggcgtggct
     3841 ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa
     3901 cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atagtgtgaa ccgggagcct
     3961 ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacaatg caggcgaacc
     4021 acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc caaggggggg
     4081 aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa
     4141 tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc catctggcgc
     4201 aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc
     4261 aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag attaattatc
     4321 tgttcatcgg cattctggca ctgctgctcg cggtcgctct ctggccatcc attccctggt
     4381 ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca
     4441 ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc ctggattacg
     4501 aactggcgaa acagtttgcc gattacctcg gtgtaaaact gaaagtgacc gtgcggcaga
     4561 atatcagcca gctgtttgac gacctcgata atggtaacgc cgacctgctg gcggcaggac
     4621 tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg tcctacctat tattccgtgt
     4681 cacaacaact ggtttataaa gtgggtcagt atcgcccacg tacgctgggc aacctgacgg
     4741 cggagcaact caccgttgca ccgggtcacg tggtggttaa cgatctccag accctgaaag
     4801 acacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct gcggaattaa
     4861 tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc gccatcagcc
     4921 tgtttcagcg cgttcacccg gaactcgccg tagcgctcga tatcaccgat gaacaaccgg
     4981 tgacctggtt tagcccgtta gatggcgata ataccctttc cgccgccctg ctcgacttct
     5041 tcaacgaaat gaatgaagac ggtacactgg cacgcattga agagaaatac ctggggcatg
     5101 gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat gcggtactgc
     5161 cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt ttgctggccg
     5221 ctattgctta tcaggaatcg cactgggatg cgcaggccac ttcaccgacg ggtgtgcgcg
     5281 gcatgatgat gttaaccaaa aacaccgcgc aaagcctcgg cattacggat cgtaccgatg
     5341 ccgaacagag catcagcggc ggcgtgcgtt atttacagga tatgatgagt aaagtgccgg
     5401 aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc cgcgtacaat atgggctatg
     5461 cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa aggtaatcct gacagttggg
     5521 ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc aagctgactt
     5581 acggctacgc tcgtgggcat gaagcctacg cttatgtcga aaatattcgt aaataccaga
     5641 ttagcctggt gggttatctg caggagaaag agaagcaggc tacagaagcg gcgatgcaac
     5701 tggcgcagga ttatccggcg gtatcgccta cggagctggg caaagagaaa tttccttttc
     5761 tctcgtttct ttcccagtcg tcatcaaact atttgaccca ctctccctct ctgctgtttt
     5821 ccagaaaagg gagtgaagag aaacaaaatt aatctgtcga ggattgcgct tttttctgcg
     5881 ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgca cactcatccg
     5941 ccagtattcc ttccgtaatt tccacccggt gattcatacc cggatgatgc agcacatcca
     6001 ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc
     6061 gactgtggat cattgctccg gcacacatta cacagggttc aagcgtgaca tacaacgtgg
     6121 cgtcgatcag acgataattt tgcatcacca gaccgccctg ccgtagggcc atgatttctg
     6181 catgtgcggt gggatcatgt cgaccaatcg gacggttcca gccttcgcca ataacacgat
     6241 tgttatgcac taataccgcg ccgaccggta cttcccgctc atcccaggca cgcttcgcca
     6301 gcgtcattgc atgacgcatc cagtattcgt agctaaattc gacttcagac aaaaagaaaa
     6361 ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc tattccagtt
     6421 gctggagttc accgcgcggg gttacccgcc agcgatgctg gcaaaagtaa agcagcgggt
     6481 tgtcctggtt actgtcgcta tagccactgt acagccgcag cggcgtgccg attttgcgct
     6541 ccagttgcgc gaccttctca tgcccgaggc aacgcatcgt caatacccaa ccaccatagc
     6601 cacgctgaat ctgactggcg ataagattaa cccgcggcag ccagggcgta tcgaaataaa
     6661 ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca tcggaactta
     6721 acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg tcacgaaacc
     6781 agcgtacgaa atcggcctgc aacgcctgca aacgcgcttc attgtgacca aaagtgcatc
     6841 cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg
     6901 ctatgatcgg caacaacggc agaacaagta acgcattcag cggttggcga cgcagtaaat
     6961 aacgcaaaaa actgccgaac atatcctgct gatgcaatgt tccatctaag tcaaaaaaca
     7021 ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat cctgattaat
     7081 tttcgctact acatagccta acagatagat catcactttt ccggcaacaa tcgaccgcat
     7141 catggctaac tggaattttt aattcatggc aattagcggc aatggaatat aaaattcact
     7201 cgcgtgtgtc tcatattgcc tgttgtcgcc attatttcat cgcgaggaaa gatatgaacg
     7261 gcttacttcg tatccgtcag cgttaccagg ggcttgccca gagtgataaa aaactggcgg
     7321 attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa ctggccaacg
     7381 aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc tataaaggtt
     7441 ttccggcact aaagttggcg ttgagtgaag cgctggcaag ccagccggaa tctccctccg
     7501 tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc gaaaaactga
     7561 ttaaagaaaa tactgccgct atgtacgcaa cgttaaacgt taatagtgaa gagaaactgc
     7621 atgaatgcgt ggcaatgttg cgctctgcgc ggcggataat tctgaccggt attggcgctt
     7681 cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc aatgctgccg
     7741 cagtgcgcga tatgcatgcg ctactcgcta ccgtacaggc gtcgtcccct gacgatctgt
     7801 tattagccat ttcctacacc ggtgtacggc gcgagttaaa cctggcggca gatgagatgc
     7861 tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tcccaatgcc ctgcaacagc
     7921 gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagt gcttcaatct
     7981 ctgcttgtca cgcgcaggga atgttaacgg atttgctgtt cattgcgctg attcagcagg
     8041 atctggaact ggcaccagaa cgtattcgtc atagcgaagc gctggtgaaa aaactggtct
     8101 gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag tttccttatg
     8161 gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga atgcccgaat
     8221 gaggcgattt cgatgggtga acatatctac gagattaaca gcgataagtg taccgaatgc
     8281 gtagggcact acgagacacc aacctgccag aaggtgtgtc cgatccccaa tactattgtg
     8341 aaagatccgc aacatgtcga gacggaagaa cagttgtggg ataaatttgt gctgatgcac
     8401 cacgcggata aaatttaata ttctgctctg gaagtagagt gttaattatt ttatagagaa
     8461 atcttaacgc tattatattt atttaaatga tgacattagc ataatcattc actaagttaa
     8521 tttatatagt atctgcccag acacttattt atagttatta aaggcgcgtc cgattggttc
     8581 accggacgca ccttaagtac gtttccttgt gttataagaa caaaaggagc agctgtaaaa
     8641 cagcaatgat gattttgatg acccgtttaa tcaggtatcg gcaatcagtc attcgttttt
     8701 ccttaaacaa ggaatgcagc cgttcagatt agcccttaca tctccccaaa actgaacgtg
     8761 cgagttattg gaatcgagtg ctgcactcca caccagagct ttgacgacac cactcgtttc
     8821 aatgggggaa ttcagtggca tggtgtaaag cacagcaaaa ttgccaataa cgaaaccgat
     8881 tttaatgtac ttatcaaaaa gcggtcacgt taatcttttc atcaatgtcg atttcttcgt
     8941 aaaggctcgc gttttttatg tcagagattg caagttgctt gtacaggtca acgaaattga
     9001 tccataatca ttttcgttgg aatcgtgtgc tgcacaaaat taaagttaaa aagtaaaacc
     9061 cccgttcctt accagttcgg gggttttact ttataaag
//
LOCUS       DAJOTB010000177         9012 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJOTB010000177 DAJOTB010000000
VERSION     DAJOTB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.177"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(212..1033)
                     /gene="dapB"
                     /locus_tag="OHN28_004220"
     CDS             complement(212..1033)
                     /gene="dapB"
                     /locus_tag="OHN28_004220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCQ2299508.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1194..1559)
                     /locus_tag="OHN28_004221"
     CDS             complement(1194..1559)
                     /locus_tag="OHN28_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2299509.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1582..2496)
                     /gene="rihC"
                     /locus_tag="OHN28_004222"
     CDS             complement(1582..2496)
                     /gene="rihC"
                     /locus_tag="OHN28_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCQ2299510.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2562..3512)
                     /gene="ispH"
                     /locus_tag="OHN28_004223"
                     /gene_synonym="lytB"
     CDS             complement(2562..3512)
                     /gene="ispH"
                     /locus_tag="OHN28_004223"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCQ2299511.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3514..3963)
                     /gene="fkpB"
                     /locus_tag="OHN28_004224"
     CDS             complement(3514..3963)
                     /gene="fkpB"
                     /locus_tag="OHN28_004224"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2299512.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(4071..4565)
                     /gene="lspA"
                     /locus_tag="OHN28_004225"
     CDS             complement(4071..4565)
                     /gene="lspA"
                     /locus_tag="OHN28_004225"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCQ2299513.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4565..7381)
                     /gene="ileS"
                     /locus_tag="OHN28_004226"
     CDS             complement(4565..7381)
                     /gene="ileS"
                     /locus_tag="OHN28_004226"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCQ2299514.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKV
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7424..8365)
                     /gene="ribF"
                     /locus_tag="OHN28_004227"
     CDS             complement(7424..8365)
                     /gene="ribF"
                     /locus_tag="OHN28_004227"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCQ2299515.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8373..8591)
                     /locus_tag="OHN28_004228"
     CDS             complement(8373..8591)
                     /locus_tag="OHN28_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCQ2299516.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8694..8957
                     /gene="rpsT"
                     /locus_tag="OHN28_004229"
     CDS             8694..8957
                     /gene="rpsT"
                     /locus_tag="OHN28_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCQ2299517.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         2247 a         2515 c         2178 g         2072 t
ORIGIN      
        1 gattgcatga aatcaaaatg agtagaaaaa agtggaccaa atggtcaaaa tttacattaa
       61 aacaacaaaa acagaataca aaacatgtaa ctttcagcat ttgcattaaa tttaaaaaca
      121 taacaccaaa aataaaaggg ccaaaaatta aagcccttta tattcaatga attaaatgtg
      181 ttatttttgc atcattataa atattttgtg gttacagatt attgagatca agcacatctc
      241 gcatatcaaa aagaccactt tccttaccac tcaaccacaa agccgatctt accgcaccgt
      301 tagcaaatgt catgcggctg gatgccttat gggtgatctc cagacgctcg ccaatatcgg
      361 caaacatcgc ggtatgttcg ccaacgatgt cacctgcacg cacggtggca aaaccaatgg
      421 tgccaggcac acgttcaccg gtatggcctt cacgactgta gaccgcgcaa tctttcagat
      481 ctttatcaag agcgtgggcg atcgcctctc ccattgccag tgcggtgcct gacggcgcat
      541 caactttatg tctatgatgt gcttcaataa tttcgatatc ggtgtagtcc cccatcactt
      601 tggctgcttt ctccagcagt ttaagcatga cgttaacgcc aacgctaaaa ttggcggcaa
      661 agacaatcgc aatatcggcg gcggcgtcac gaatcgcctg tttaccggct tcgtcaaacc
      721 ccgtagtgcc gatcaccatc cctttgccat gctggcgaca aaaagcgaga tggttcagcg
      781 taccttccgg acgggtaaaa tcgataaaca catcaaaatc atcttttatc gcatcaagac
      841 tactttgcac agtaacgcct gttttcccgg ctccggccag ttcgcccgca tcgctgccca
      901 gtaaagaaga tccttcgcgc tccagcgccg cgcccagctg cacgccttct aatgccagcg
      961 ccgcttgaat caactggcgg cccatacggc ccccggctcc cgcgatggca acgcggatgt
     1021 ttgcatcatg catagctatt ctcttttgtt aatttgcata gaccgttttc agagtaacca
     1081 gcgcatcgct acgccgccag ccaaaaacgc tgataattag aaattaatga taaacaggga
     1141 gggatatcag tgaaaggcat gacgcagaaa gtgatgcgtc ataccctctg gcattatgcc
     1201 tggtcttgtg actttgacgg caggcagcac agtataagca gaattacgcc cgtaacaggg
     1261 atcaacacga caatggtaac ccaccaccct gaatatccga tatcgtgcat ccttctgacg
     1321 gctaacgcta tcgccggtac ggcaaaaatc aacgttaaaa taaatgacgc gggcagcgca
     1381 aaaaaaatga aaacaccata gtcattgtta attgttaccg ctaaccacca acataaaagc
     1441 ggaagcagac agataacgcc actaccaatc ataaacgacc agaactcgaa gcgattggaa
     1501 acaccttcat aattaaagat gtttttccac ccttccagat acgcttctcc atacgtcata
     1561 gaacacttcc ttgttttatt attagagcgc cagggccagc acctcagcca cccactgctg
     1621 gaagcctttc acatccagat ccagcgccac ctgtacattg gctggtttgc ccaggcaacc
     1681 gtcgatatca acaaccgtcg tgcctgaggt aaattcaccc tgagtttcta ccgcgacaaa
     1741 acacggtttg agtgtgaaca attccgggcg caccagccag gcgatagcgc aaagatcgtg
     1801 catacgtagt ccactttgca tactgccgct acggtagtgg ctaaacaggg cgtgtagcat
     1861 ttttccggtg cgattcaact ccggcaaagt agcgagatag tcaggggtta atattgcctg
     1921 attggtgaca tccagaccgc acatgacgat ttcgatgccg ctacggaaga cacaggctgc
     1981 cgcttctgga tcggcagcga tattaaactc ggcgtttggt gtgcagttgc cgcgaccagc
     2041 agaaccgccc atgatcacca gacggcgaat atacggctta cattccgggc attgtgaaag
     2101 taacagcgca atattggtta acggaccgat agccaccagg gtaacgggct ctggcgcacg
     2161 catcagggca tcacgaattg ccagaaacgc cggtataccc agtggctgac ggttgtgttc
     2221 aacaaagtta tagccagcca ttcccgattc accgtgtaca gatgccgcat cgcgcggcgc
     2281 acgaaccagt ggtacggcag cgccctgggc aagcggaata tccacattcc agaaatgcag
     2341 cagttgcagg gcgttgcggg tggttttctc taccgaaaca ttacccgcga ctgtggtcat
     2401 cagttgcaga tcgagttcgg gggtaaaaat cgcggcggca atggcaacgg catcgtcaat
     2461 gccggggtct gtatcgagga agataggtaa atgcatgttt tctccataaa aaatgccggt
     2521 aacaagaccg gcattttcgc ataacttaga ctactaatga cttaatcgac ttcacgaata
     2581 tcgacacgca gctctttcgg cacttcgaag acgatatttt cttcgcggcc ttccagcgga
     2641 atggcttcgc caccgccaag ctgctgcaag cgtgccacca cattctgcac cagaatatcc
     2701 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctgg
     2761 atatctttcg catcgtcaat caaaaatgcg cgtttgccca tacgctgggc cagttccgcc
     2821 agacggttgg agttggagga gtttttcgaa ccaaccacca gtactacttc cgcctgctct
     2881 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
     2941 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
     3001 accgacagcg tggtttgggt cataaaggag agcttctctt catttttgac cgtcagtttc
     3061 cacacatcgt ctggcgattc gaccagatac attccccctt ccgggttgct gtactggccc
     3121 atcgtccctt ccacttccgg gtgcccggcg tgaccgatga gaatagattc ttcaccacga
     3181 cggctggcgc gggcgacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
     3241 accgtcaaat cgcggctttt cgcttcgtta cgtactgcct gagaaacacc gtgtgcggag
     3301 aaaatcagga tcgcgccgtc cggtacttcg ctaatctgct caataaagat agccccacgc
     3361 tcgcgcaggc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
     3421 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
     3481 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
     3541 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
     3601 aatggagtcg ccgttaattt cgcggatcac gccaggcatc tcactgccat ccattgcggt
     3661 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
     3721 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
     3781 ggttttatcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
     3841 atcacccagg cggaacagcg ccggtttacc gttgttgcgg gtagactcag cggtggtgcc
     3901 atcatcgagt ttcagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
     3961 catgcaggtt tgctcttata ttttgaggat ttttgccgga tgcggcctac gatttcgcag
     4021 caatctgtag gccggataag atgcgtcagc atcgcatccg gcagggttta ttattgtttt
     4081 ttcgctttag aaggtaaaaa accttccagc acaatcagtg ccgcaccgac acaaatggca
     4141 gtatcggcaa ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc gatcatatcg
     4201 acaacgaagc cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc aataatcagc
     4261 gcgtaagcga tattgtttag cttctgcgcg gccttcgagc gatacatcat cactaccagg
     4321 atcacgctaa taccaatcgc aataccggca aagaaccaac gctgccagcc gccgctatcg
     4381 gcaaggaaac taaacgccgc gccatagtta cgcgcataat gcagattaag cgacgggaac
     4441 agcgggaccg tatcccccag agcaaagttc tggaggatca ggtatttgct gcccagatcg
     4501 ataatcagca cgactaccac cagccacagc cagcgtagcc ctgttgaaca gatcgattga
     4561 ctcatcaggc aaacttacgt ttttcaccgt caccggcgac gttgctgaca cagcggccgc
     4621 agatttctgc gtgttccgcc accttgccga catcctgggt gtagtgccag cagcgagggc
     4681 acttctcacc ttcggcttta ctcaacgcga ctttcagccc tttgaggact tcgctctgct
     4741 gggcatcagc aggtgcgtcg ttatagtctg caacggtagc gccggaggtc aacaggacaa
     4801 atcgtaattc atcgcccagc gcggtcagtt tcgccgccag ttccggttct gcatacaagg
     4861 ttactgcagc ttccagcgag ccgcccactt tcttatcggc acgcgcttgc tcaatgactt
     4921 tgttcacttc gccacgcact ttcaacagct cgtcccagaa cgcatcgttc attgcttcac
     4981 tgtctgccag accaaacagg ccttcgtacc actcgccggt gaagacgtat ttttcacgtt
     5041 cgcccggcag gtagccccac acttcatcag cggtgaagga gaggattggt gccatccagc
     5101 gaaccagcgc ttctgcgatg tgatacagcg cagtctggca gctacgacgc gccacgctgt
     5161 ccgctttggc ggtatactga cggtctttga tgatgtcgag gtagaaggaa cccatctcaa
     5221 cggagcagaa gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct tcgtacgcct
     5281 tgaggatgtc ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc agtaccacca
     5341 tctcttccgg tttcaccata tcttttgccg gatcaaaacc gttcaggttt gccagcagga
     5401 agcgcgcggt gttacggata cgacgatagc tgtcggcagc acgtttcaga atctcgtcag
     5461 aaacagccat ttcgccggtg tagtcggttg atgccaccca cagacgcaga atatccgcac
     5521 ccagtttgtt catcacatcc tgcggcgaaa cggtgttacc gatggattta gacatcttgc
     5581 gaccctgacc atccacggta aagccgtgag tcagtacctg acgatatggc gctttgccct
     5641 tcatcgcggt agagatcatc agggaagaca tgaaccagcc acggtgttgg tcagaacctt
     5701 ccagatacat gtccgctgcg tgaccggcaa attccggacg cacatcaaca acggaagagt
     5761 gggtagatcc ggagtcaaac catacatcca gcgtatccgg tactttcaca tactggtcag
     5821 cttcgtcgcc gaggatctct ttcgcatcga gatcccacca cgcctggatg ccgtcaactt
     5881 caacgcgttt tgccacttct tccatcagtt cgagagtacg cggatgcagt tcttctgtgt
     5941 ctttgtgcac gaacagtgac atcggcacgc cccaggtacg ctgacgagag atacaccagt
     6001 caggacggtt agcaaccatc gactcgatac gcgcctggcc ccagtcaggg atccactgca
     6061 cgcctttgat ctctttcagt gactgcgcac gcagaccttt ctgatccatg ctgacgaacc
     6121 actgcggcgt cgcgcggaag atgatcggcg ttttatgacg ccagcagcac ggatagctgt
     6181 gttgcatttt ctcaacgtgc aacagtgcgc ctttttcctg caacaacgca atgacaatat
     6241 cgttcgcttt gaagacgtta acgccatcca gagtcgggta agtacccggc agataagtgc
     6301 cgtccgggcc aaccgggtta gcggtttcca gaccatattt ttgaccgatc acatagtcgt
     6361 ccggaccgtg gcctggcgcg gtatgaacgg caccggtacc ggcatccagc gtaacgtggt
     6421 cgccaagaat tgccggaaca tcgaagtcca taaacggatg ggtaaagcgc aacagttcca
     6481 gctcagcacc tttcaccgtg ccaagaatgg tgtaatcgct aacgccgata cgctgcatta
     6541 cgctttcaac cagaactttc gccagaatca cggcctgacc gtcgatttgc accagcgcat
     6601 aatcaaaatc aggtgcaatg gagattgcgc ggttagcagg cagcgtccac ggcgtggtgg
     6661 tccatataac cagcgaaatt gggccgttaa cattgcttac gccaaatttc gttttcagcg
     6721 catcctgatc gaccgcctgg aaagcgacgt cgatggacgg agaagttttg tcgtaatact
     6781 caacttccgc ttctgccagt gcagaacggc agtcaacgca ccagtgcacc ggcttcgcgc
     6841 ctttgtgcag gtgaccgttg ccgatgattt tgcccagcgc gcggatgatg ttggcttcag
     6901 ttttgaagtc catggtcagg tacgggtgcg accagtcgcc cagcacgccc agacggataa
     6961 agtctttgcg ctgaccgtca acctgggtcg cagcgtattc gcggcacttg gcgcggaact
     7021 cagcggcggt gaatttctcc cccggcttac cgtattcttg ctctactttc agttcgattg
     7081 gcagaccatg acagtcccag ccaggcacat acggcgagtc atatccagaa agccctttgg
     7141 acttaatgat aatgtctttc agaatcttgt taaccgagtg accaatatga atgctgccat
     7201 tcgcataagg agggccatca tgcagaatga aggttttttt gcctttttta gctgcacgaa
     7261 tgatgccgta cagatcatca tcagtccaac gcgccagcat tcccggttcg cgcttggcga
     7321 gatcgccacg catcgggaac cctgtttccg gcaaattcag ggttgattta tagtcactca
     7381 tcagattctc ggttccgtat ttcggtttga ttacataaca ggcttaagcc ggttttgtta
     7441 gcccaaaaaa ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc agttcgtcca
     7501 gcgatgcaaa tcgctgctca ttgcgtattt ttttacgcag cactacttgt atatggcgac
     7561 cataaaggtc cattgcaaca tctaacaaat gcacttccag ttgctggcga ataccggcaa
     7621 ccgttgggcg tgttccgatg tttgcaacgc cgggtaacgg cttttcgcca aggcccaaca
     7681 cttctaccgc ataaacccct ttcaccgggg aaacctgacg gcgtagcggt acattcgccg
     7741 tcgggaaacc tatagtgcgc cctaattcat caccgtggac tacacgcccg gagatggcaa
     7801 acgggtgccc cagtaaactt tctgccagag ccagattgtc atccgccagc gcctgacgca
     7861 cggcggtgct gctgatacgc acgccacctt cgcaaaaagt ttgcgtgctg gtgatatcga
     7921 agccgtattc catacccgct ttctgtaata acaagaaatc gccttcacga ccagcgccaa
     7981 agcggaaatc gtcacctacg gcaagaaatt ttacccgcaa gtgcttcacc agaagatcac
     8041 tgatgaagtt ttgcgcggtt aacgccgcaa aacgcctgtc aaaacgcacg cacagcacgt
     8101 aatcaacgcc acactctgca agataacgca gtttttcccg cagccgggtc agccgtgccg
     8161 gggctttatc agtagcaaac agttccagtg gttgaggttc aaaaagcatc accatcaccg
     8221 gtaagttgcg cttgcgccct tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat
     8281 gcacgccgtc gaaattacca atagtcagca cacacccttc ttgcggggcc tggctgagat
     8341 tatgtatgcc gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata
     8401 ccttgtacag cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa gaagtggctt
     8461 ttcattgcta tggcgcaaat catgggaaga aactgaccgc ctactgcaat ttttctcgcg
     8521 gaaaagctgt attcacaccc cgcaagctgg tagaatcctg cgccatcact acgtaacgag
     8581 tgccggcaca ttaacggcgc ttatttgcac aaatccattg acaaaagaag gctaaaaggg
     8641 catattcctc ggcctttgaa ttgtccatat agaacacatt tgggagttgg accttggcta
     8701 atatcaaatc agctaagaag cgcgccattc agtctgaaaa ggctcgtaag cacaacgcaa
     8761 gccgtcgctc tatgatgcgt actttcatca agaaagtata cgcagctatc gaagctggcg
     8821 acaaagctgc tgcacagaaa gcatttaacg aaatgcaacc aatcgtggac cgtcaggctg
     8881 ctaaaggtct gatccacaaa aacaaagctg cacgtcataa ggctaacctg actgcacaga
     8941 tcaacaaact ggcttaattg cctgttgttg tcagctttgt aaaaaaaccc gctctggcgg
     9001 gtttttttat gc
//
LOCUS       DAJOTB010000178         8919 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJOTB010000178 DAJOTB010000000
VERSION     DAJOTB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.178"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            70..1212
                     /locus_tag="OHN28_004230"
                     /pseudo
     CDS             70..1212
                     /locus_tag="OHN28_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(1219..1983)
                     /gene="glcC"
                     /locus_tag="OHN28_004231"
     CDS             complement(1219..1983)
                     /gene="glcC"
                     /locus_tag="OHN28_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCQ2299518.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQSRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2234..3733
                     /gene="glcD"
                     /locus_tag="OHN28_004232"
     CDS             2234..3733
                     /gene="glcD"
                     /locus_tag="OHN28_004232"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCQ2299519.1"
                     /translation="MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3733..4785
                     /gene="glcE"
                     /locus_tag="OHN28_004233"
     CDS             3733..4785
                     /gene="glcE"
                     /locus_tag="OHN28_004233"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCQ2299520.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYLDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTSWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4796..6019
                     /gene="glcF"
                     /locus_tag="OHN28_004234"
     CDS             4796..6019
                     /gene="glcF"
                     /locus_tag="OHN28_004234"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCQ2299521.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6024..6428
                     /locus_tag="OHN28_004235"
     CDS             6024..6428
                     /locus_tag="OHN28_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCQ2299522.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6450..8621
                     /gene="glcB"
                     /locus_tag="OHN28_004236"
     CDS             6450..8621
                     /gene="glcB"
                     /locus_tag="OHN28_004236"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCQ2299523.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
BASE COUNT         2124 a         2247 c         2548 g         2000 t
ORIGIN      
        1 ttattaagat gccgttcgtt gctgttgcaa cctgtttttc accgttaatt atttgttatc
       61 cacagagata tggaatgcga tctgctgatg attaaaattg aaaaagtaat taataaaaat
      121 gaccttaagg catttatcgc ttttccgtca tcactttatc ccgatgatcc aaactggata
      181 ccccctttat tcattgaacg cagcgagcat tagtctgcga agaatccggg gacggaccat
      241 atcatctggc aggcgtgggt ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg
      301 caaatcgata ccttgcaccg tgagcgttac ggtgaagata ccggtcattt cggaatgatt
      361 gatgccaatg atgatccgca ggtttttgcc gcgttgtttg gcgcagcgga agcgtggtta
      421 aagtcacaag gtgcaagtaa gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc
      481 ggattactga ttgaaggttt tgacacacct ccctgtgcga tgatgccata cggcaaaccg
      541 tggtatgccg cgcatattga acaactgggt tatcacaaag gcattgattt actggcgtgg
      601 tggatgcagc gagccgatct cactttctct ccggtgctaa aaaaactgat ggatcaggtg
      661 cgcaaaaaag tgaccattcg ctgcatcaat cgccagcggt ttgccgaaga gatgcagatc
      721 ctgcgtgaga ttttcaattc tggctggcag cacaactggg gatttgtgcc gtttaccgaa
      781 catgaattcg cgaccatggg cgatcaactt aaatatctgg tgccggacga tatgatctac
      841 atcgctgaga ttgattctgc accctgcgcg tttattgttg gcttgccgaa catcaacgag
      901 gcgattaccg atctgaacgg atcgctcttt cccttcggct gggcaaaatt gctgtggcgt
      961 ttgaaagtca gcggtgtgcg aactgcgcga gtgccgctga tgggcgtgcg ggacgagtat
     1021 cagttcagcc gcatcggccc ggtgattgcg ctgttattga ttgaagcctt acgcgatccg
     1081 tttgcccgcc ggaagatcga tgcgctggaa atgtcatgga tcctcgaaac caatacaggc
     1141 atgaataaca tgctggaacg cattggggcg gagccgtaca agcgttaccg attgtatgaa
     1201 aagcagattt gatggcagtc acccctgctt catctccagc ggcaccgccg agcgaatcaa
     1261 atggtggcct tccagctcga tatcgtggag attctttttc acggtccgca catgatcgcg
     1321 tgctgcgcgc tgggcaacgt gcggcagccg ctgcaacacc gcgttgtaga tccgcgcatg
     1381 ctggcgatcg atctgctttt tttgctgtgg tcgatggtag agattattta cgctggcaaa
     1441 caccgaatta aacatcaggt cggtcaatga ttgcagcgta aacaccagca cctgattgtg
     1501 agaagcctga caaatggcga gatggaaagc gtgatccaac tgcgcatgtt cgatcagcga
     1561 aatctctttg ttgttctcac tggcggcgag cattttttca taacagcggg ttatcacaac
     1621 aaaatcagcc tgggttccca gcgttgccgc cagccttgcc gattcgccct ccagtaatgc
     1681 gcgaacgtcg agcagatcgt acagcgttcg cgactgcgta ctgaacagat ggatcagcgg
     1741 gctggcgtcc tgcacccgat taagccgtgc gacacgagaa tcgcgaccct gcgccgtttc
     1801 aataatcccg cgcccgcgca gcacggtcag cccttcacgc agtgcggagc gtgagaagcc
     1861 gagcttttca cacagtcgac gttccgaggg aagcggctga ccgaccttca gtacgccgtc
     1921 gataattaac cgttcgatac tttctgcaac cacttcgcaa atagggcgac gttcatcttt
     1981 cattcccgga cctcgtgcac aggtaggacc aatttttctg ttcttaactc gcaaaacacg
     2041 cacatcacgt aagtgattat gttacatcaa tttaacatta agttaaccaa agcaaggtca
     2101 cagagctgga aaaaaaatgg tctgaccggt agctaaagag atagacgaaa acgaaaagcc
     2161 cgcttaataa ctgttcacag aagcagcgcg aaaaaatcag ctgccacaca acacaacaaa
     2221 gcgaacccta ctcatgagca tcttgtacga agagcgtctt gatggcgctt tacccgaagt
     2281 cgaccgcaca tcggtactga tggcactgcg tgagcatgtc cctggactgg agatcctgca
     2341 taccgatgag gagatcattc cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc
     2401 attactggtt gttctgccta agcagatgga acaggtgacg gcgattctgg ctgtctgcca
     2461 ccgcctgcgt gtgccggtgg tgacccgtgg cgcaggcacc gggctttctg gtggcgcgct
     2521 gccgctggaa aaaggtgtgt tgttggtgat ggcgcgcttt aaagagatcc tcgacattaa
     2581 ccccatcggt cgccgcgcgc gcgtgcagcc gggcgtgcgt aacctggcga tctcccaggc
     2641 cgttgcgccg cataatctct attacgcgcc ggatccgtcc tcacaaatcg cctgttcgat
     2701 tggcggcaat gtggcggaaa atgccggtgg cgttcactgc ctgaaatatg gtctaaccgt
     2761 acataacctg ctgaaaattg aagtgcaaac gctggatggc gaggcgctga cgctgggatc
     2821 ggacgcgctg gattcgcctg gttttgacct gctggcgctg tttaccggat cggaaggtat
     2881 gctcggcgtg accaccgaag taacggtgaa gctgttgccg aagccgcccg tggcgcgggt
     2941 tctgttagcc agctttgact cggtagaaaa agccggactt gcggttggtg acatcatcgc
     3001 caatggcatt atccccggtg ggctggagat gatggataac ctgtcgattc gcgcggcgga
     3061 agattttatt catgccggtt atcccgtcga tgccgaagcg attttgttat gcgaactgga
     3121 cggcgtggag tctgacgtac aggaagactg cgagcaggtt aacgacatct tgttgaacgc
     3181 gggtgcgact gacgtccgtc tggcacagga cgaagcagag cgagtacgtt tctgggccgg
     3241 tcgcaaaaat gcgttcccgg cggtaggacg tatctccccg gattactact gcatggatgg
     3301 caccatcccg cgccgcgccc tgcctggcgt actggaaggc attgcccgtt tatcgcagca
     3361 atatgattta cgcgttgcca acgtctttca tgccggagac ggcaacatgc acccgttaat
     3421 ccttttcgat gccaacgaac ccggtgaatt tgcccgcgcg gaagaactgg gcgggaagat
     3481 cctcgaactc tgcgttgaag ttggcggcag catcagtggg gaacatggca tcgggcgcga
     3541 aaaaatcaat caaatgtgcg cccagttcaa cagcgatgaa atcacgacct tccatgcggt
     3601 caaggcggcg tttgaccccg atggtttgct gaacccagga aaaaacattc ccacgctaca
     3661 ccgctgtgct gaatttggtg ccatgcatgt gcatcacggt catttacctt ttcctgaact
     3721 ggagcgtttc tgatgctacg cgagtgtgat tacagccagg cgctgctgga gcaggtgaat
     3781 caggcgatta gcgataaaac gccgctggtg attcagggca gcaatagcaa agccttttta
     3841 ggtcgccctg tcaccgggca aacgctggat gtgcgttgtc atcgcggcat tgttaattac
     3901 gacccgaccg agctggtgat aaccgcgcgt gccggaacgc cgctggtggc gattgaagca
     3961 gcgctggaga gcgccgggca aatgctcccc tgcgagccgc cgcattatgg tgaagaagcc
     4021 acctggggcg ggatggtcgc ttgcgggctg gcggggccgc gtcgcccgtg gagtggttcg
     4081 gtccgcgatt ttgtcctcgg cacgcgcatc attaccggca ctggaaaaca tctgcgtttt
     4141 ggtggcgaag tgatgaaaaa cgttgccgga tacgatctct cacggttaat ggccggaagc
     4201 tacggttgtc ttggcgtgct cactgaaatc tcgatgaaag tgttaccgcg accgcgcgcc
     4261 tccctgagcc tgcgtcggga aatcagcctg caagaagcca tgaatgaaat cgcccagtgg
     4321 caactccagc cgctacccat tagtggctta tgttacttag acaatgcgtt gtggatccgc
     4381 cttgagggcg gcgaaggatc ggtaaaagca gcgcgtgaac tgctgggtgg cgaagaggtt
     4441 gcaggtcagt tctggcagca attgcgtgaa caacaactgc cgttcttctc gttaccaggt
     4501 acctcatggc gcatttcatt acccagtgat gcgccgatga tggatttacc cggcgagcaa
     4561 ctgatcgact ggggcggggc gttacgctgg ctgaaatcga cagccgatga taatcaaatc
     4621 catcgcatcg cccgcaacgc gggcggtcat gcgacccgct ttagtgccgg agacggtggc
     4681 tttgccccgc taccggcacc tttattccgc tatcaccagc agcttaaaca gcagctcgat
     4741 ccttgcggcg tgtttaaccc cggtcgcatg tacgcggaac tttgaggagc aggctatgca
     4801 aacccgatta acagaagaga tgcggcagaa cgcgcgcgcg ctggaagccg acagcatcct
     4861 gcgcgcctgt gttcactgcg gattttgtac cgcaacctgc ccaacctatc agcttctggg
     4921 cgatgaactg gacgggccgc gcgggcgcat ctatctgatt aaacaggtgc tggaaggtaa
     4981 cgaagtcacg cttaaaacac aggagcatct cgatcgctgt ctcacttgcc gcaattgtga
     5041 aaccacctgt ccttctggtg tgcgttatca caatttgctg gatatcgggc gtgatattgt
     5101 cgagcagaaa gtgaaacgcc cactgccgga gcgaatgctg cgcgaaggat tgcgccaggt
     5161 agtgccgcgt ccggcggtct tccgtgcgct gacgcaggta gggctggtgc tgcgaccgtt
     5221 tttaccggaa caggtcagag caaaactgcc tgctgaaacc gtgaaagcta aaccgcgtcc
     5281 accactgcgc cataagcgtc gggttttaat gctggaaggc tgcgcccagc ctacgctttc
     5341 gcccaacacc aacgcggcaa ctgcgcgagt gctggatcgt ctggggatca gcgtcatgcc
     5401 agctaacgaa gcaggctgtt gtggcgcggt ggactatcat cttaatgcgc aagagaaagg
     5461 gttagcgcgg gcgcgcaata atattgatgc ctggtggccc gcgattgaag caggtgccga
     5521 ggcaattttg caaaccgcca gcggttgtgg cgcgtttgtc aaagagtatg ggcagatgct
     5581 gaaaaacgat gcgttgtatg ccgataaagc gcgtctggtc agtgaactgg cggtcgattt
     5641 agtcgaactt ctgcgcgagg aaccgctgga aaaactggca attcgcggcg ataaaaaact
     5701 ggccttccac tgcccgtgta ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa
     5761 agtgttgctt cgtcttggat ttaccttaac ggacgttccc gacagccatc tgtgctgcgg
     5821 ttcagcggga acatatgcgt taacgcatcc cgatctggca cgccagctgc gggataacaa
     5881 aatgaatgcg ctggaaagcg gcaaaccgga aatgatcgtc accgccaaca ttggttgcca
     5941 gacgcatctg gcgagtgccg gtcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca
     6001 agcccttgaa aaggaataac aaaatgaaaa ctaaagtcat tcttagccag caaatggcga
     6061 gtgcaattat tgccgcaggt caggaagagg cgcagaaaaa taactggtct gtttccattg
     6121 ctgttgccga tgacggcggt catctgctgg cgttaagtcg catggacgat tgcgcgccga
     6181 ttgcggctta catctcccag gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta
     6241 agggctatga agagatggtg aacaacggac gtaccgcgtt cgtgactgcg ccgttactga
     6301 cgtcgctgga aggtggcgta ccggttgttg tggatgggca aattattggt gccgtgggcg
     6361 tttctggttt aaccggagca caggatgcgc aggtcgcgaa agcggcagca gcggtgttgg
     6421 cgaaataagc gaaaacgagg agataaacaa tgagtcaaac cataacccag ggccgtttac
     6481 gcattgacgc caattttaaa cgttttgtgg atgaagaagt tttacctggc gtggaactgg
     6541 atgctgccgc gttttggcac aatgttgatg agatcgttca cgatctggcg ccagagaatc
     6601 gtcagttgct ggcagagcgc gatcgcattc aggcggcgct tgatgagtgg catcgcagca
     6661 atccggggcc ggtaaaagat aaagcggcct ataaatcttt cctgcgtgaa ctgggctacc
     6721 tggtgccaca gcctgaccac gttacggtgg aaaccacggg catcgacagc gaaatcacca
     6781 gtcaggcggg gccacagctg gtggttccgg cgatgaacgc ccgctacgcg ttgaacgcgg
     6841 cgaacgctcg ctggggctca ctgtacgatg cgttatacgg cagcgacatc atcccgcagg
     6901 aaggggcgat ggtcagcggc tacgatccgc agcgtggtga gcaggttatc gcctgggttc
     6961 ggcgtttcct cgatgaatca ctaccgctgg aaaatggcag ctatcaggat gtggtggcgt
     7021 ttaaggtggt cgataaacaa ttacgcatcc agttgaaaaa tggtaaagaa accacgttac
     7081 gtactccagc acagtttgtc ggttaccgtg gcgatactgc tgcgccgacc tgcattttgc
     7141 tgaaaaataa cggcctgcat attgagctac aaatcgatgc caacgggcgg attggcaaag
     7201 acgattcggc gcacatcaac gatgttatcg tcgaagcggc catcagtacc attctcgact
     7261 gcgaagattc ggttgcggcg gttgatgcgg aagataaaat cctgctgtac cgcaacctgc
     7321 tgggcctgat gcaggggact ctgcaagaga aaatggagaa gaacggtcgg caaatcgtgc
     7381 gtaaactgaa tgacgatcgt cagtacactg ccgccgatgg atctgaaatt tctctgcacg
     7441 gacgctcgtt gctgtttatc cgcaacgtgg gtcatttgat gaccattcct gtgatttggg
     7501 acagcgaagg caatgaaatc ccggaaggca ttcttgatgg cgtcatgact ggcgcgattg
     7561 ccctctatga tttaaaagtg cagaaaaact cgcgcactgg cagcgtctat attgtgaaac
     7621 cgaaaatgca cggtccgcag gaagtggcgt tcgccaacaa actgttttcc cgcgttgaga
     7681 caatgctcgg tatggcaccg aataccctga aaatgggcat tatggatgaa gaacgccgga
     7741 cctcgctgaa cttgcgtagc tgtatcgctc aggcgcgcaa ccgcgtggcg ttcatcaata
     7801 ccggtttcct cgaccgtacc ggcgatgaaa tgcattcggt gatggaagcg ggcccgatgc
     7861 tgcgtaaaaa ccagatgaaa tcgacgccgt ggatcaaagc ctacgaacgt aataacgtgc
     7921 tttccggtct gttctgtggg ctacgtggaa aagcgcaaat cggtaaaggc atgtgggcaa
     7981 tgccggacct gatggcagac atgtacagcc agaagggcga ccaattgcgt gccggggcaa
     8041 acacggcctg ggttccgtcg ccaactgccg ctacgctcca tgcgctgcac taccaccaaa
     8101 ccaacgtaca gagcgtgcaa gccaacattg cccagaccga gttcaatgct gaatttgaac
     8161 cgctgctgga cgatctgctg actattccgg ttgctgaaaa cgctaactgg tcggcgcaag
     8221 agatccaaca agagctggat aacaacgtgc aggggattct ggggtacgtg gtgcgttggg
     8281 tggagcaggg gattggttgt tcaaaagtgc cggatattca caacgtggcg ttgatggaag
     8341 accgcgcaac gctgcgtatt tccagccagc atatcgccaa ctggttacgt cacggtattc
     8401 tgaccaaaga tcaggtgcag gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa
     8461 acgctggcga tccggcttat cgtccgatgg cggggaattt cgctaactcg tgtgctttta
     8521 aagctgcctg cgatttgatc ttcctcggcg tgaaacagcc aaacggctat accgaaccgt
     8581 tattacacgc atggcgttta cgcgaaaaag aaaatcatta atagcgtcaa aaaaagcccc
     8641 ggcaagattt attgtcgggg ctatgatagt aactgcggta gtacatttat cccggaaata
     8701 accggagcga ttatcagggg ttaacaatat taaatacccg tagcgcatca ctagcgggat
     8761 taaaacaaca atatggacat cttaacgata ctctgcgcct gcgtgtgcgg aacagaaacc
     8821 cttacccgat atacttgtta tgccagaggt agcaattgcg ttagcggcgt actgttgctg
     8881 taaagtataa cgcctatatt ttacagttca gtcctcttt
//
LOCUS       DAJOTB010000179         8917 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJOTB010000179 DAJOTB010000000
VERSION     DAJOTB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.179"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            212..1036
                     /gene="gadX"
                     /locus_tag="OHN28_004237"
     CDS             212..1036
                     /gene="gadX"
                     /locus_tag="OHN28_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCQ2299524.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1405..2133
                     /gene="gadW"
                     /locus_tag="OHN28_004238"
     CDS             1405..2133
                     /gene="gadW"
                     /locus_tag="OHN28_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCQ2299525.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2496..5609)
                     /locus_tag="OHN28_004239"
     CDS             complement(2496..5609)
                     /locus_tag="OHN28_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCQ2299526.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5634..6791)
                     /gene="mdtE"
                     /locus_tag="OHN28_004240"
     CDS             complement(5634..6791)
                     /gene="mdtE"
                     /locus_tag="OHN28_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCQ2299527.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6851..7180
                     /locus_tag="OHN28_004241"
     CDS             6851..7180
                     /locus_tag="OHN28_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299528.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7130..7657)
                     /gene="gadE"
                     /locus_tag="OHN28_004242"
     CDS             complement(7130..7657)
                     /gene="gadE"
                     /locus_tag="OHN28_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCQ2299529.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8456..>8917)
                     /gene="hdeD"
                     /locus_tag="OHN28_004243"
     CDS             complement(8456..>8917)
                     /gene="hdeD"
                     /locus_tag="OHN28_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCQ2299530.1"
                     /translation="CISFPFVSGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLS
                     GFLVAVAYLLIGYFFIRAPELGIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQL
                     VIGVLDIVIAWIFLGATPMVSVTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2372 a         2065 c         2069 g         2411 t
ORIGIN      
        1 acgatgatga attatttgct tactattaat tcctctgcgt tcttaagtcc cctttttata
       61 taataaatcc agtcatccag cccgagtatc atcgctcatt atcatcctgt tttcccgctt
      121 cgtttaaatt tatttatcaa tcaatttgac ttaagagggc ggcgtgctac attaataaac
      181 agtaatatgt ttatgtaata ttaagtcaac tatgcaacca ctacatggta attgtctaat
      241 tgcgtacgca agacataaat atattctcac catggttaat ggtgaatatc gctattttaa
      301 tggcggtgat ctggtttttg cggatgcaag ccaaattcaa gtagataagt gtgttgagaa
      361 tttcgtatta gtttcaagag acacgctttc attatttctc ccgatgctca aggaggaggc
      421 attaaaactt catgtacata aaaaagttcc ttcattactc gttcatcact gtaccagaga
      481 tattcctgtt tttcaggaag ttgcgcaact atcgcagaat aagaatcttc gctatgcaga
      541 aatgctacgt aaaagggcat taatctttgc gttgttatct gtttttcttg aggatacgca
      601 gtttataccg ttacttctga acgtgttgca accgaatatg cgaacacgag tttgtacggt
      661 catcaataat aatatcgccc atgagtggac attagcccga attgctagcg aattgttgat
      721 gagtccaagc ctgttaaaga aaaaattgcg cgaagaaggt acgtcatatt cacagttgct
      781 tactgagtgt aggatgcagc gtgctttaca acttattgtt atatatgggg tttcaattaa
      841 gcgagttgca gtatcctgtg gatatcacag cgtgtcgtat ttcatttacg tttttcgaaa
      901 ttattatggg atgacgccca cagagtatca ggagcgatcg gcgcaggaat taccgaactg
      961 tggctcggcg gcaagtatgg ctgcgcaagg gattttttac ggcactgacc gttctgcgga
     1021 aggaataaga ttatagagtt ttactcagac ataaaaaaaa cccggcatag gggaccggga
     1081 agaggatagt ctgccgtctc cagactaata aaccgttata acactccctg ttggcacggg
     1141 aaactttgtg ctctcagtaa gttaaatata acttttactg gaaataagat cagccatttt
     1201 tttataaaca taagctatac gctgtgtgaa aatataatcg tgttgcattt acttattatc
     1261 aattaactgt tatgcaaaac tactttgtgg ataaaatttg gtcctaccaa atatggcagt
     1321 ttttgcacac agaaacagtc tggcgtcatt tcattagtat actgacattg aaataatcgc
     1381 agtaatgaaa tataagggat agtcatggct catgtctgct cggtgatcct cgttcgtcgt
     1441 tcattcgata ttcatcatga acagcaaaaa atatcgttgc ataacgagag tatcctgctg
     1501 ctggataaaa atttggcaga cgattttgcg ttttgttcac tggatacgcg acggctggat
     1561 atcgaagagc tgacagtttg ccattactta caaaatattc gtcagttgcc acgcaattta
     1621 ggattgcata gcaaagaccg tttgttaatt aaccagtcac cccccataca gctggtgacg
     1681 gcgatttttg atagtttcaa tgacccccgg gtcaattcgc cgatactgag caaaatgctc
     1741 tatctttcct gtttatcaat gttttctcat aagaaagaac tgatcccctt acttttcaat
     1801 agtatcagta ctgtttcagg aaaagttgaa cgccttatta gctttgatat cgctaaacgt
     1861 tggtatctac gcgatatcgc agaaagaatg tacaccagcg agagtctcat caaaaaaaag
     1921 ttgcaggatg aaaatacctg tttcagtaaa atattactcg cctccaggat gtcgatggcc
     1981 agacgattac tcgagttacg tcaaatacct ctgcatacta ttgcggaaaa atgtggctat
     2041 agcagtacgt catactttat aaatacattt agacaatatt atggtgtaac gccacatcag
     2101 ttttcgcaac attcgccggg taccttttcc tgacatattt cgcatttgaa tattggtcag
     2161 gatctcacgt ctgcttcatg tgaaactctt tcctgatgat ttctgccggg ctaccggcta
     2221 gttctctttc gcagtttgcc aggcggtata ggtattttca ggaggaaaag actcggcatg
     2281 tttgggatta ttaagctaac aattcatacg attacgggaa tgtgggtcag tattgtgctg
     2341 ttcaaattga tgacgaatgg ctggagtggt ttctactttc agtgctgtgt actaagcctg
     2401 gtattcctga cggttagctg gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct
     2461 gaaccctcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
     2521 agatgttcca ctacaacgaa aaagaccgga acgaagtaaa ttgccagcac tgttgcggca
     2581 aacatcccgc ccattacgcc ggtacctacc gcgttttgcg cgccagagcc ggcaccatga
     2641 ctgataacca gcggcagcac gccgagaata aaggccagag aagtcatcag gattgggcgt
     2701 aaacgcatac gcgctgcttc gatgattgcc tctaccggtg ttttcccttc tttctgcatc
     2761 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
     2821 agcaaaccaa cctggaagta aacgtcatta cttaagccgc gcagatcggt ggccagtaat
     2881 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctttca
     2941 taaagtgcgg cgagggcgag gaacaccacg accagtgaaa tggcatatag cgcgggagcc
     3001 tgatttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
     3061 ggaagttttg cgaccaggtc tgccataaat ttcatggcgt caccggtact tttcccggca
     3121 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccag tcgcggtgaa
     3181 ccataggtcc attcggtaga cgaataggca gaaagcggtg ccatcgtgcc agaggcgttg
     3241 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcc tgcctggaca
     3301 tacacttttt tcacccgccc ctggttgagg aagtcgttca cgtagctgct gccgaaggcg
     3361 gtggaaattg tctggttgat atcagacagc gccacaccca tagcctcggc tttcgcagcg
     3421 ttgacgttca ctttgaacat cggcgtatct tccaggccgt tcgggcgtac cccgatgacc
     3481 tgattcggtg attgcgctgc cagtgataac agctcgtttc gcgcctgggt cagtttttcg
     3541 tgccccagat tgccgttgtc cagcagttcc atatcaaaac ctgacgcggt acccagttca
     3601 gccaccgcgg gtaagttgaa cgggaagacg acggctttat tgatactgct tagcgcaatc
     3661 attgcccgct gaataatcgc ggtaaccgag ttttcctcgc cgacacgttc agaccacggc
     3721 ttgaggctga taaacgccag accgttgttt tgcccctgac cactgaagcc aaagccacca
     3781 acggtaaaca ccgactgaac attatttttc tcttttgtca gataataatc cgtcacctgt
     3841 tgtagcactt tcgtggtgtt aaccatggtt gcaccggaag gtaactgcgc ggtggtcata
     3901 aataccccct gatcctcttc tggtaagaaa gaggtcggcg tacgcaggaa cagcaccgcc
     3961 atcccggcgc aaatcagtag gtagaccacc atgtagcgac cggtacaacg caacagcgag
     4021 cgggtgctgt cggtatagtg ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac
     4081 agggcgttag gtttgtgacc gccctccggc gcggctttca gaatggtggc gcacagggca
     4141 ggggtcaggc tcattgctac aaatactgaa agcagcatgg aggagatcag cgtgatggag
     4201 aactggcggt agatctcccc ggtcgcaccg ctcataaagg ccatcggcat aaacactgcg
     4261 gaaagaacaa cggcaatccc gaccagcgca cgttggatct gccccatcga tttatgcgtc
     4321 gcttccttcg gcggtagctt atcttccgca atgacacgct cgacgttctc caccaccacg
     4381 atggcgtcat ccaccagtaa ccctatcgcc agcaccatcc cgaacatagt taacgtgttg
     4441 atggtgaaac caactgccga caagatcgca aacgtcccga gaataaccac cggtacggca
     4501 atcgtcggga tgattgtggc gcggaaattc tgcaaaaaca gatacatgac caggaagacg
     4561 aggatgatag cctcaaccag cgttttgaaa acttcctgaa tagaaatttt gataaacggt
     4621 gtggtgtcgt aaggataaac cgtcttcagg cttgcaggga aataagccga taaacggttc
     4681 agctcctctt tcaccgctcg cgaggtatcc agggcgtttg ctccggtagc cagtttgatg
     4741 gcgatcccgg cggcaggttt gccgttatag cgcgccacgg tggaataatc ttccgcccca
     4801 agttcgacgc gggcgacatc acgcagcagc acttgcgaac catcttgctg aactttcaac
     4861 aggattttgc caaattcttc cggcgtttgc agacgcgtct gcacaatgat cgaggcgttc
     4921 agttgctggt ctgccgcctg tggcatgcca cccagttgac caccagaaat ctggttgttt
     4981 tgcaccttaa tctgggaaat aacatcggaa ggtaccaggt tatatttatt gagtttttgc
     5041 gggtccagcc agatacgcat ggcgtactcg gaaccaaaga gctgtacgct accaacgccc
     5101 gcggtacggc ttagcgggtc tttgatatta gacgctacat agtccgcgat atcgtactgg
     5161 ttgaggctgc cgttatcaga aataaatgcc gctaccatca ggatattact gctcgactta
     5221 tcgacgctaa tcccctgctg ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt
     5281 ttattttgca cctgaacctg tgcgatatca ggcgatgtcc ccgtctcgaa ggtcagagtg
     5341 atggaggcat tacctgccgc atcgctggtt gaagacatgt acatcaggcc atcaagccca
     5401 ttcatatttt gctcaatcac ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc
     5461 ggataggttg cgctgacggt aatggtcggt ggcgcaatct gcggatactg cgcaaccggt
     5521 aagttcatga tcgccagacc gcctgcaagc atcataataa tggcaagtac ccaggcaaaa
     5581 accgggcgat caataaaata gttagccatg aaagtcccct taagcctgca acgttattgt
     5641 ttcgattcgg tgctggcgtt ttcctggctg gaggaaattg ctcgtgcttt gatacccgga
     5701 cgaatgcgtt gcaagccgga aacgatcacc cgatcgccag cctgcaagcc agaggtgaca
     5761 acccactggt cgccgatggc tttgctggct tcgatttcgc gtagctgcac gacatcgtct
     5821 ttatccagaa tgagtgccgt tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc
     5881 accagtaata cattctggcg gctaccttca tccactaatg ccgtaacata catgccaggc
     5941 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
     6001 tccaccgtcg ggtcggagaa tttcagcgtg ccggtctggc cgtaacgttt accattttcc
     6061 agattgagct gtactggcgt actgccctga acctgtttga tttgcccact ggcgacctct
     6121 tctttcatgc gcaagaaatc ctgcaccgac tgcgtgagat cgacataaat cgggtccaga
     6181 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
     6241 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
     6301 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcaactgg
     6361 gtgcgcgcgg tgtcgtaatc ctgacggcta acatagttgg tcttcagcaa cgatgcctgg
     6421 cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag cgagcctttg
     6481 gcggagttca gctctgcctg taaaggcgca ggatcaatct gatacagcga atcgccctgg
     6541 ttcactttat cgccttcgat aaagttgcgt ttaatgataa ttccgcccac ctggggacgt
     6601 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac attgaccgaa
     6661 ccgggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt ttccgccgat
     6721 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagttttctt
     6781 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     6841 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaagat
     6901 gggatgcaag cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     6961 gccaacagtt tctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     7021 ggggaataaa gtcgttttcg tctctccttc aataatcacc gggggtattg aaatcagcat
     7081 ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtccttac taaaaataag
     7141 atgtgatacc cagggtgacg atgtcgctca tacgtcttaa tttcagcttg tacataatgt
     7201 tgaatttctg aatctttaat gtttgttgcg aaatgttgag cgttgacgtg atattgcttt
     7261 catttttgtt tttcagaatc agttgcataa ttttgaactg attatgtgaa aaacatagtt
     7321 gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt
     7381 ctttacggct gacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg
     7441 ccaaaacaat aattctctcg gcatctaatt tctccaggaa tttaatcgct tcttcatcaa
     7501 ggatatggtt gtgataggtg tcaatgatca ccttgaaggg tttattgcct acttttttga
     7561 tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct
     7621 gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg
     7681 tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag
     7741 taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata
     7801 cttcagattt atccatcacg cactccatat attggtccta agtaagattc ctggttgtta
     7861 tcagtttgta actagtaaac aaaaggttaa cttttgcgga ttactgccca agaataagtt
     7921 atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt
     7981 aaatatcgag tctccttgtt tcgacttagg ctggcaattg gattgccagc tttcttttaa
     8041 cgcattgaaa ttacctgatt aaataactca ttcatgtctt caggttttta tatagcgtct
     8101 ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt
     8161 acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc
     8221 acttttaaaa cattaaacct gaccacggaa aatatcagcc atgataatgt ttgaaatggc
     8281 taatttgcca tggaacgaaa aaaatcagat taaattcagt aggttgaaat aatcaacagc
     8341 aggcaattgt ttcaatgata gtgcgtaatt gaactgaaac actgtatagc taagagagta
     8401 ttaactggat catagtcact taatgtgact atgatccagg tggaaatagg gataattatt
     8461 gctttttaac gaacaaactg gcgaagctga acagactggc ggcgctaaat atcagttcaa
     8521 ttcccaccag tgtggaaacc agcgttaccg acaccatcgg cgtagcacca aggaatatcc
     8581 aggcaatgac gatatccagc acaccaataa cgagctgtag ccaactgcct ttcattgaac
     8641 gctgacgata ccaactcatc aggcgaataa cccctgccac acagaacaaa ccggcaataa
     8701 atgccgcaat ggcaaaaatg cccagctctg gtgcgcggat gaagaaatag ccgatcaata
     8761 aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc
     8821 tgaatagccc gacaataagc gcaatgcccg agcagattaa taatgcaccc actactgtgc
     8881 ttaaaatgtc gccagagacg aacggaaaac tgataca
//
LOCUS       DAJOTB010000180         8712 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJOTB010000180 DAJOTB010000000
VERSION     DAJOTB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.180"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(401..1291)
                     /locus_tag="OHN28_004244"
     CDS             complement(401..1291)
                     /locus_tag="OHN28_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299531.1"
                     /translation="MFISDETIRIIHLVARHQNITTAAEQLNKVPSTISYTLKKAEET
                     LGVELFIRKGRYIELSPAGSYFIEHSKNILRDLDALKRNTVLIHDGIERELTIAVNNI
                     IPGDFLVSFIQDFERQFTSTTLTVDIEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQIEWDFVVSPLHPLAVKRNPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFAMAISLIEQNVGIGYIPHHLALPLLNRGKLLKKPMREHKHATKLFLAARSD
                     GIGKACLWCMSYLRNKQFMA"
     gene            complement(1369..2592)
                     /locus_tag="OHN28_004245"
     CDS             complement(1369..2592)
                     /locus_tag="OHN28_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2299532.1"
                     /translation="MMRKEDNMNTASGLRITMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQNGIEAPLALLVAAGCVIFVASTAFGFSILGKVAQVAVPVIAL
                     VLCYILYVATHTEVSAPAAIAEMSTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGMTFLVAYPLLYWAGATPSAISGQGSLLNAMAVFGAVMPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLSRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLTQESQVKVLTFAAWITGTAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2615..3004)
                     /locus_tag="OHN28_004246"
     CDS             complement(2615..3004)
                     /locus_tag="OHN28_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HCQ2299533.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGAHVDVEIWQLPLASFAELLMNEPEGLTIGKIKLADGSEVLGVLA
                     ESWLTEGQKEITALGGWRKYTGNFHTM"
     gene            complement(3021..3977)
                     /gene="arcC"
                     /locus_tag="OHN28_004247"
     CDS             complement(3021..3977)
                     /gene="arcC"
                     /locus_tag="OHN28_004247"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2299534.1"
                     /translation="MVKPLAVVAVGGNALIQDDKRNSIPDQYVAVMESVQHIVDMVEA
                     GWELVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPYDDAFASPSKPIGAFLDEETAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHHTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAIHFGTPQQQWLDAISVYEAQTLLQEGQFGVGSMQPK
                     VEAIIDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3970..5394)
                     /locus_tag="OHN28_004248"
     CDS             complement(3970..5394)
                     /locus_tag="OHN28_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2299535.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGHAISLNWQPPAQGDID
                     TGLVLASLLRHPLVESANQIAMTRYLEAQPVLVDVMLAKEAIPEMAEQKRILHSGPPI
                     AWEDMCGPVKGAIIGAMLYEGWATSQKDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAASDLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDACKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRELAEIVAKENHHG"
     gene            complement(5391..6950)
                     /gene="fdrA"
                     /locus_tag="OHN28_004249"
     CDS             complement(5391..6950)
                     /gene="fdrA"
                     /locus_tag="OHN28_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2299536.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALLLAQQRLTSKPDDETDCGIKSPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKYIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETHVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNDQPLPAVENEVSCDDLTMLQ
                     NACQSLPVHRQAIRGVFAGGTFCYEAQLICQQKGFHAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGTSATPVSEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISSLQNAGVIIANSNAQAALWASTVA
                     QTQLQKKELNA"
     gene            complement(7045..7905)
                     /locus_tag="OHN28_004250"
     CDS             complement(7045..7905)
                     /locus_tag="OHN28_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2299537.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEALPLVFQHYPTIASQRLLFCLEHNVQSTLHLPDSLTHQGLDIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLANHQYRLQKAIEQHAHNTT
                     DISRHYLQRALQGHFSEPICRLLAQLVGSASAMTIASCTEQIMQFGATSGVNCLAGML
                     HGFRTLNTMN"
     gene            complement(7911..8579)
                     /locus_tag="OHN28_004251"
     CDS             complement(7911..8579)
                     /locus_tag="OHN28_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCQ2299538.1"
                     /translation="MTISIFQAQPFELPFDPCTTALIMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCKEVLAAARQKGIMVIHTREGHREDLSDCPSAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVTGEPVIDKPGKGAFYQTDLHLILQKRGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
BASE COUNT         2178 a         2172 c         2119 g         2243 t
ORIGIN      
        1 tgtcaattta agtttattat tatgattttt tatttttgta ctatgaatct cctgtatgct
       61 tatatgtgca gaaaatctca ttttatcaag aagaatatca aattttctcc gatacttcta
      121 catactccat cattcatgct ccttttatcc gtgcaccgta aaaccccgtc cttcagcggg
      181 gatataaggc gctgttttcc acctaaccag gtgtctgctg ctattcaatg tattggcgga
      241 tgacggatgc cgcccgttga agcaggaacc ctcgtcctta gggcgtggag gatgtcaaaa
      301 tgaaaacttg tggtatgaat caatccattc atcatccttt cggatcattg cattcagtat
      361 aaccctggaa gagcctggca atatcactgt attgatggta ttacgccatg aattgcttat
      421 ttcttaaata cgacatgcac cacagacagg cttttcctat accatcggaa cgagcggcaa
      481 gaaataactt ggtggcgtgt ttatgttcgc gcattggctt tttaagcaat ttacctctgt
      541 tcaataacgg caatgccaga tgatgtggaa tataaccaat accgacgttt tgttctatta
      601 agctgattgc catggcgaag tcaggaacaa aaataggttt ttgtccttcc aacaaccagg
      661 cttgcattgg ggggaagtta acggaggtat cccggataca aaccgcagga taatgacgca
      721 attcactgtt ttctaatgga ttccgcttca ctgccagtgg atgaagtggg ctgacgacaa
      781 aatcccattc tatttgtccg accggttcgc taatgatgcc ttcactgctt ggtactgcat
      841 gaggagcacc gtagaccaga tcagcacgtt tactgtacag cgcatcccag cagccgttgt
      901 atacttcaat atctaccgtc agtgttgttg atgtgaattg ccgttcaaaa tcttgaataa
      961 atgacaccag aaaatcacca ggtataatat tgttcaccgc aattgtcagt tcacgctcaa
     1021 taccatcgtg aatcagcact gtgttgcgct ttaatgcatc cagatcgcgg agaatatttt
     1081 tgctatgttc gatgaagtaa ctccccgccg gagataattc aatatatcgt cccttacgaa
     1141 tgaacagttc aacgcccagg gtttcttccg ctttcttcag ggtgtaactg atagtagaag
     1201 ggactttatt taactgttcg gctgcggtgg tgatgttctg gtgtctggcg accagatgaa
     1261 taattcgaat cgtttcgtct gagatgaaca taaggctccc tggtcagtgg taaagtggct
     1321 gaatctctcc agattcagcc gtaatcattg cctgagtata cccaggaatt agcggtgttg
     1381 acttgcccga ctgagaatcg catacgcgat acatgccacc agaatggagt caactgaagg
     1441 gatcgtcgtc agggtgaata agccttttac ggtcataaag ccaaccgctg tgcctgtgat
     1501 ccatgcagcg aatgtcagta ctttaacctg tgactcctga gttaacaccg actcttgata
     1561 gccattacgg cgataaagga aaaagtcagc gatataaatt ccggcaactg ggggagtcgc
     1621 gatacccagg aacagcagaa acggaataaa ccacgccata atatccatac tgcctacgat
     1681 ggcggaaagg atacccagcg caacggtaat ctgccatttg ggaaagcggc taagcagtgt
     1741 ggaaactacc aacgtcccct ggaacatatt gcccgcatta ccggtgacgc aggcgaatat
     1801 cagcaacagt gctgcaggca taaccgcacc gaatactgcc atagcattca gtaaagagcc
     1861 ctggccgcta atggcactcg gcgttgcacc cgcccagtat agcagagggt aggcaaccag
     1921 aaacgtcata cctgcggcaa tcagagcatg ttttcggtta tgtacaaaac taccgaagtc
     1981 gggaagtgta gcaaccagca caataatagt gccaacaacg gtggaaacgg cgacacctgt
     2041 actcatctcc gctatcgctg ctggcgctga tacttcggta tgggttgcca catagagtat
     2101 ataacacagc accagtgcga taaccggcac ggcgacctgg gcaactttgc ccagaatgga
     2161 aaaaccaaag gccgtagagg cgacaaaaat gacacaacca gctgccacta acagtgccag
     2221 tggcgcttca atcccattct gcgccagtaa gtcatgcacc gaatgaccaa acatattagc
     2281 ggtaacggca atccagccga acagacttat agccatcaaa atattaatgg cgatggcccc
     2341 tttttcgcca aatgaatatt tcacaatacc gtatgttggc aggcgggctt tttctcccac
     2401 actaatggtg atggctgaaa gtaaggtcag aatgacgccg cctaatagta cgacgctaat
     2461 taaactacca ctggaaagtt gattgccgag gcttgaggat gacaacagca caggtgtgac
     2521 ggcaataccc agcaaaatca tggtaatacg caatcctgat gccgtattca tattatcttc
     2581 tttacgcata atatgtttcc tttataaatg cgcgttacat cgtgtgaaaa ttcccggtgt
     2641 atttgcgcca gccccccagt gctgtaatct ccttctggcc ttcagtcagc cagctttctg
     2701 ccagtacgcc cagtacttcg ctaccatcgg caagtttgat ttttccaatg gtcaggcctt
     2761 cgggttcgtt cattaacagt tcggcaaaac tggccagagg cagttgccag atttctacgt
     2821 ctacgtgtgc tcctccttca ttcacgcgga tcattccggg atgacgatca ttaatactcc
     2881 ataaccggta atgggcgtca gttcggtctt cacggacaaa aataccgccc gctttttgca
     2941 tatttgggtt gagttccaaa ccgcgcatca gcgttccgtt aacggctaaa agtgttgtag
     3001 tcattgtgtt ctccagagaa ttaaagggtt atccatgttc cgctctgatg tgccagggct
     3061 gcttttatgg tttgcggtga agtaatcagt ccgctggcct gtttgccttg ttgctggctg
     3121 gcattgatga aatcaataat ggcttccact ttgggttgca tactgccgac accaaactga
     3181 ccttcctgca acagcgtttg agcttcgtag acgctgatag cgtcgagcca ttgttgttgt
     3241 ggtgtaccaa agtgaatcgc cactttttct acaccggtgg ggatcaccag cagatcagcg
     3301 cctaactgac tggcgagcaa tgcggaggcc aggtctttat cgatcacggc ttccacacct
     3361 ttcagttgtt gcccgtctcg caccacaggt ataccgccgc cgccgcaggc tataaccaga
     3421 tatccctggc gcacaaggtg agcgatggtg tggtgctcaa taatttccag tggcgcagga
     3481 gagggaactg tacgccgcca accacgcccg gcgtcctcca tcagcgtcca gccgagttgt
     3541 tgttggcgtt gttgtgctgt ttcttcatca agaaacgcgc caatcggttt actggggctg
     3601 gcgaaagcat cgtcatatgg gctgactcgc gtttgtgtca ccagggcaat taccggtttg
     3661 tttatgccac ggcgagccaa ttcgttatgc agcgctttct ggaacatgta tccaatggcg
     3721 ccttgggtat cacccacggc gtaatccaga ggaaccggag aaacttcgtt actggcaagt
     3781 tcagagcggc gcagaataaa gcctacctgc gggccattac catgggttag caccagctcc
     3841 catccggctt caaccatatc aacgatatgc tgcacgcttt ccattactgc aacatattga
     3901 tcgggaatac tattgcgttt atcgtcctga atgagcgcat tgccgccaac cgcgacgaca
     3961 gccagtggct taaccatgat ggttctcctt tgcgacgatc tccgccagtt cacgtacagc
     4021 agcgacaaaa caggccatcg gtgcagtggt gatcccggca ccaatttgtc cgactcccgc
     4081 ttgtttgtgg gcgatacccg tattgatgac cggtaaaatg ccacgatcgg tcactttaca
     4141 cgcatcaata cctgctgcgg tgggggcaaa attgagggct ggaagtgtaa atgcggggtt
     4201 accgccgagg gtgattgcct gcatccgacg gctgttattg gtggcatcgg caggggtgcc
     4261 tcccacaaat ttaacaatcg ccggagatga cgccatcgcg aagccaccca cacctgcggt
     4321 ttcagtaatt gcgctatcac ccaggtcaga agcggcgtcg tcaacgccaa atccggggaa
     4381 gaacagacct tcaactgcat tggcaggagc ctgaaaccag cgatcgccag taccggaaag
     4441 ccgaatacca aaattcaccc cgttacgcgc catcaccgta accattgaac tgaacggtac
     4501 attggcggcg gcatccatca ttgctttaca ggcagccatc gacaaattga ggaagaagtg
     4561 atcgttacct gtgataaatg ccacgacgcg ctggatatgt tcctgtggaa gagagcaggc
     4621 cagcaatgcc gggagcaggc gcttaatcag caaaccggta gcggcagcat tgcggttatg
     4681 aacttcatcc cccatatgca gcgcctgcgc cattaatggc ttgagttcca gctcgccgtg
     4741 ttgtgcgatt gctgctttca tggccggcgc cagttcatca cgcatccagg ccagacgatt
     4801 gagaacttca tcgttattgg caccaaaacg gagaacctta cccagaccct cattgaaatt
     4861 gctaaaagta cgatggccgt tggttttgtt ttccaccacc cataatggca tcgacgggct
     4921 gataatgcct gccattggtc caaccgcgtg ataatgatga caaggagcaa ggtcgatttc
     4981 gcctgcgttt atctggtttt ctgcgtcttt ctggcttgta gcccagcctt cgtacagcat
     5041 ggcaccaata atcgcccctt ttaccggacc acacatatct tcccaggcaa tcggtggacc
     5101 agagtgtaaa atgcgttttt gttcagccat ttcggggatg gcttctttag cgagcatgac
     5161 atccaccaac acgggctgtg cttcgagata acgtgtcatg gcgatttggt tggcactttc
     5221 taccagtgga tggcgtagta atgacgccag aaccaaaccg gtgtcaatat caccttgtgc
     5281 tggcggctgc cagttgaggg atatagcatg ccctcctgca tgttgaatat tgtcagcaaa
     5341 actgtgtagc cccgcgttca cgaccttcaa aggctgatta aataaggtat tcatgcattt
     5401 agctcctttt tctgcaactg agtctgtgca acggtgctgg cccatagcgc cgcctgtgca
     5461 ttgctgttgg caatgatgac gcctgcattt tgtagtgagc ttatctgctg actgcgtatt
     5521 tgcggatcag cttctgttcc gcaaacgtgg gcaatcagca acggtgcatt attcatgtcg
     5581 atgtgtgaaa gttggtccag taattcgctg acaggcgtag cggaggtgcc ataaccaagt
     5641 acaaggtcga ataacacaac ggcagtatgt gagtcgttga gttcgttgag cagacgttga
     5701 ttgcgcagag tcgggtcgat cattggatgc ggtttgccac gggtgaaatc atcgtccccc
     5761 atatcaatca gtgtatgatc ttcactttgc cagatatttg ccagagcacg attacccgca
     5821 actggcgtat tggaggcggc atggaatccc ttttgttggc agataagctg agcttcgtaa
     5881 cagaaggttc ctccggcgaa aacaccgcgt atcgcctggc gatggactgg cagactttgg
     5941 caggcgtttt gtaacattgt gagatcgtcg caggaaacct cgttctcgac agccggaagt
     6001 ggctgatcat tcagtaacgc cacagcgatg tcggcggcgc tggcaagtgt ggtggcggca
     6061 gtgatattgg gacgtgccag atcatgcgga ttcgccccga ggaaattcac caccacgggt
     6121 tttccacaag cctcggcgcg ctccagaata gtccgggcaa cgatcggtga aggcggtttt
     6181 gaaataagca caatcacgtg agtttcatcg tcttgtgcca gcgcatctaa agcaaagagc
     6241 attgatattc caccaatctc ttcactgaga tcatgtccgc ctgttcccaa cgcctgagaa
     6301 atacccgctc cgagctggtc gattcggcaa gtcacttcct gtagcccggt gcctgacgcg
     6361 ccaataacac caattgctcc gcgtttcacc acattagcga agcctaacgg aatgccgtta
     6421 acaatggcgg ttccacagtc aggtcccatt acgatgcgct gacgttctcg ggcaagcgtt
     6481 ttgatgtatt tttcctgccc gatactgacg ttatcgctga acatcatcac gttcattccg
     6541 aggtttaacg ctttaattgc ttcggcggcg gcgtagtctc ccggtacaga aatgagtgcc
     6601 aggttggctt cgggttcagc ttccagtgcc atttccaggc tgaccttttc cggtgattta
     6661 atgccacaat ccgtttcatc atcgggtttg ctggtcagac gttgctgggc cagtagcagt
     6721 gcttcattgc aaatatcttc ttcacccatg actgcaatca ctaaatcatt gggactggcg
     6781 ttgatttcgt tacccagacc cgcatcgcgc aattgctcga gattattggg tgttcccatg
     6841 acgaccgagg cctgttgaat accgggcaac ttgctgattt gcgcggaaat ctgcatcaga
     6901 gaaactgagt cctgataaag attactgaaa actttatatt taactttcat gctgttacgc
     6961 ctgttccatt ccgcccgata gcaaaattcg ggcaaagcat ccccgtgtag ccccataaac
     7021 gggctacggg agaaaaggtt gaaatcaatt catggtgttc agggttcgaa atccatgcag
     7081 cattcctgca aggcaattta ctcctgatgt tgcgccaaat tgcatgatct gttctgtaca
     7141 ggatgcgatt gtcattgccg atgcgctccc aaccagttgt gcgagtaacc ggcaaatcgg
     7201 ttctgaaaaa tgtccctgaa gcgcacgttg cagataatga cgacttatat ccgtggtgtt
     7261 gtgtgcatgt tgttcaattg ccttctgcaa cctgtattga tgattggcaa gtggtgcagg
     7321 gagttgccat aaccagaggg ctgccagata gcccagcaaa tagtcatctc catcgggcgt
     7381 taagccttta ccaaagccga tcagttgggg tacctggcgt tccagcgcat ctggatgttc
     7441 cataatatct aatccctggt gcgtgagact atccggcagg tgcaatgtgc tttgaacgtt
     7501 atgctccaga caaaataaaa gccgttgcga tgctatcgtg gggtagtgct ggaaaaccaa
     7561 aggcaatgcc tcaagagtca gcattccccc cacaaatggc gactgccaga gcgttgcgtt
     7621 atcgagcgcc acacaaaatc gaggtgtccg cagtaagcct ggggttaatt gaatgggatc
     7681 tgaatgggca atctctcttc gccagtccca acattcaggt agccagactc tggcagcatc
     7741 agggagattc agatacgttt gcgcacacaa cagagtgatg aatgttcccg cgtctgaata
     7801 cagatttacc gcatgtttaa aagcgctatg tagacgtaaa tcttgcgttt cattctgcgg
     7861 gaaaagatat cccaacgacg atgcacaaag cgtaacctgt accatcggcg ttagccttcc
     7921 agacctgcaa taatggcttt ggaatcagta acccaaccga aaatggcacc ttgtgctttg
     7981 atcatctcaa gggcgtattt ctggaattcc gggaaatagg agccaacgca atcttccggt
     8041 atgatgcatt catagccacg gtcatttgct tcacgcacgg tggtggttac gcaaacttcg
     8101 gtagtcacgc cacaaacaat aagtgtcttg ataccacgct tttgcaaaat cagatgtaag
     8161 tcggtttgat aaaaagcgcc tttaccgggt ttatcaatca caggttcgcc tgtcaccgga
     8221 tacagttcag gaatgatgtc atgacctgct tcacccctga ccagaatgcg tcccatcggt
     8281 cctggttcac caataaaggt ttttccgccg cgcgtcagtt tggctgatgg gcagtcgctt
     8341 aagtcttcac gatggccttc acgggtatga ataaccataa ttcctttttg acgtgcggct
     8401 gccagaactt ctttacatgg tgcgatagcc gtgcgaacca gtgaaacatc attacccaat
     8461 gcttctccaa accctcctgc ttcgacaaaa tcacgttgca tgtctatcat gatcagtgcc
     8521 gttgtgcacg gatcaaaggg taattcaaag ggttgagcct gaaaaatgga tatcgtcatg
     8581 acaaatctcc agtatatatt gtttactggt gacgagtatt caatataaag gtgacccaca
     8641 acagaaaaaa aagttgtttt acaggagcga atatttttga ggtgtctaaa tgttgttatg
     8701 aaaaatgtga tt
//
LOCUS       DAJOTB010000181         8635 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJOTB010000181 DAJOTB010000000
VERSION     DAJOTB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.181"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            104..1882
                     /locus_tag="OHN28_004252"
     CDS             104..1882
                     /locus_tag="OHN28_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCQ2299539.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1921..2400)
                     /gene="xdhC"
                     /locus_tag="OHN28_004253"
     CDS             complement(1921..2400)
                     /gene="xdhC"
                     /locus_tag="OHN28_004253"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCQ2299540.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2397..3275)
                     /gene="xdhB"
                     /locus_tag="OHN28_004254"
     CDS             complement(2397..3275)
                     /gene="xdhB"
                     /locus_tag="OHN28_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCQ2299541.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3286..5583)
                     /gene="xdhA"
                     /locus_tag="OHN28_004255"
     CDS             complement(3286..5583)
                     /gene="xdhA"
                     /locus_tag="OHN28_004255"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCQ2299542.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5997..6752
                     /locus_tag="OHN28_004256"
     CDS             5997..6752
                     /locus_tag="OHN28_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCQ2299543.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6831..6904
                     /locus_tag="OHN28_004257"
     tRNA            6831..6904
                     /locus_tag="OHN28_004257"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6863..6865,aa:Gly,seq:ccc)
     gene            complement(7221..8450)
                     /locus_tag="OHN28_004258"
     CDS             complement(7221..8450)
                     /locus_tag="OHN28_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2299544.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         2269 a         2026 c         2127 g         2213 t
ORIGIN      
        1 tgtttttatc aaagccaatt tcgcctgagt taatatcatc atgatctgtg aaccatcaac
       61 gtcttcaatg atcgactttt aagtaagtct ggaaggtaaa gctatggagc ttgcgactac
      121 gcagtcagta ttgatgcaaa ttcaaccaac aattcagcgt tttgccagaa tgcttgccag
      181 cgttttgcag cttgaggttg agatcgttga tgaaaacttg tgtcgcgtcg ccggaacggg
      241 cgcgtatggg aagtttcttg gccgccagtt gagcggcaac tcacgtctgc tccgccacgt
      301 cctggaaacg aaaactgaaa aagttgtgac acagtctcgc ttcgatcccc tttgtgaagg
      361 ttgcgatagt aaagaaaatt gccgcgaaaa agcatttctg ggtacgccag tcattttaca
      421 ggatcgttgt gttggggtga taagtttgat tgccgttacc cacgagcaac aagaacatat
      481 cagtgataat ttacgcgaat tttccgatta cgttcgccat atatccacca tttttgtttc
      541 taaacttctg gaggatcagg gaccaggaga taacatcagt aaaatattcg cgaccatgat
      601 cgataatatg gatcagggcg tgttagtggt tgatgctgat aatcgggttc agtttgttaa
      661 tcagacagct ttaaaaacac tcggtgttgt acaaaataat attattggaa aacctgttcg
      721 tttcagacca ttaacatttg agagtaattt tacccatggt catatgcaac atattgtttc
      781 gtgggatgat aaaagtgaat taatcattgg tcagttgcat aacatccagg gccgacaact
      841 atttttaatg gcgtttcacc aatcgcatac cagtttttct gtagcaaatg cacctgatga
      901 accgcatatt gaacaattgg ttggcgagtg ccgtgttatg cggcaattaa aacgactcat
      961 tagccgtatt gcacccagcc catccagcgt tatggtggtt ggtgaaagcg gcacgggtaa
     1021 agaagtcgtc gcccgtgcaa tccataagtt gagcggaaga cggaataaac cctttattgc
     1081 tatcaactgt gccgcgattc cggaacagct tctggagagc gaactgttcg gttatgttaa
     1141 aggcgcattt actggcgctt ctgccaacgg taaaacaggg ttgattcagg cggcgaatac
     1201 gggcacgctg tttctcgatg aaattggtga tatgccatta atgttgcagg ctaaattact
     1261 gcgcgctatt gaggcgcgtg aaattctgcc gattggtgcc agtagcccaa tacaagtcga
     1321 catccgcatc atttctgcaa ctaatcagaa tttggcccag ttcattgccg aaggtaaatt
     1381 ccgcgaagat ctcttctacc gacttaatgt tatcccgata actcttccac cgctgcgtga
     1441 acgtcaggaa gatattgaat tactagtgca ttacttttta catctgcata cccgtcgtct
     1501 gggatcggtt tatcctggca ttgctcctga tgtcgtcgaa atattgcgta agcatcgttg
     1561 gcccggaaac ctgcgcgagt taagcaattt gatggaatat ctggttaacg ttgttccttc
     1621 aggtgaagtt atcgacagca cgctattgcc gccaaatctg ctgaataatg gcacaacgga
     1681 gcaaagtgat gtaacagagg ccagtgaggc gcacctgtca ctcgatgatg cgggcggcac
     1741 ggcgctggaa gagatggaaa agcaaatgat ccgcgaggcg ctttcacgtc ataacagcaa
     1801 gaaggaagtt gctgatgaac tgggcatcgg cattgctacg ctctatcgca agattaagaa
     1861 atatgagttg ttaaacacat aacctctgag aatccggcct gaattcaggc cggatatctt
     1921 ttactttgtt ttctcgcaat ccagaactgt atttacaatc atctgatacc ccgtgcagcg
     1981 acaaagattt cccgccagtc cacgacgaat ttccgtaatg gttaatggct tctcgcgtgg
     2041 tttcgccagc attgccgtgg tagccataat caggcccggc gtacaaaacc cgcattgcac
     2101 tgcaccagat ttcgcataag ccagttggac atgagaaagt ttaccgcctt tcgcttcacc
     2161 ttccagcgtg cggatctctt ttccttcagc ccaggccgca aggaataagc aactgtctat
     2221 cgcagtgccg tcgaccagca ccgtacaggc accacattca cccacgcagc acccttgttt
     2281 gacacttagc agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg
     2341 aagctgaaaa ggcatcccgt taatggtgca ttcgatggta attgtttcgc tgtgattcat
     2401 tgcaatttcc cccccgccgc ggcgacggct tcgctaatca cttttttggt catcgtctgg
     2461 atgagatgca gacgaaactc tttactggcc cgccatgacg aacgcggggc gacatcttgc
     2521 aggactgatt cgctgatggc ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc
     2581 tgttcggcat gttggcagcg aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat
     2641 tcgctgaaat tgccgttatc cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc
     2701 attgcgtcgc gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc
     2761 ggaaaatgaa aggcgacaag gatttcgtca tgctcaagag acactttgcc cggcccggtg
     2821 tgaaagccat taatcgggac gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg
     2881 tcataaatta gcgttggcgt ggcagaatct gcgctggtgg caccgttgca aatatttccg
     2941 ccgtaggtgg cgacattacg gatctgcggc ccggcgattg atgcagccgc agcacataac
     3001 gccgggagat gacgttgagt tacaggatct tcaatgagct gggtaaatgt cgttgcagag
     3061 ccgatacgta gcgcgccatc ttccgccagc gtaattcccc gcagctccgc cagattatga
     3121 atatcaacaa tatggcgata acggtcattg tgatggtgaa gctgtatcag tacgtcagtg
     3181 ccaccggcga gcagtttggc ctgcgggttg tcagtcagca ggttgatggc atcggcaagg
     3241 gttgctgcgc gatggtaaga agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc
     3301 cagatggaat tcttcatata atcgttttgg cgttagcggc agtgtattga ttgcaacacc
     3361 ggtagccatc ttcaccgcgt tacgaatagc agcggctaca ggaattatgg gtggctcacc
     3421 cagtgactta tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg cgctttccag
     3481 ttgtggcaga tccggcatgg tcggcatttt gtaatccagc agattggggt tacggaccac
     3541 gccgcttttc gcatcgatga tcatctcttc aaatagcgcc cagccaatgc ccatccccat
     3601 tccgccgtgt acctgacctt ctgccagcag cggattaaga atatggcccg aatcatgaac
     3661 gttgaggatg cggttgatgg ttactttaca cagtgcaata tcgaccgtca gatcaacaaa
     3721 ggtacagcca aacgccggtg ggttagtggt ggttttgatg gagctttcgg cagagagctg
     3781 cccgccgcgt tcagggtggt agaaagcgtc catcgccaaa tcttttaacg acattaacgg
     3841 ctcttccggt cgttcaacca gcacgatatg gccttttatc agggtcagat tcatcgctga
     3901 ctgatgtagc atgactgcgg cgtgagcgat gattttctct tttaataaca gtgccgcact
     3961 gcgcagcgca ggcgcggcaa catagctctg acgtgaggca aatgcgccgg ggtcgaatgg
     4021 tgtaacgtcg gtgtcttgtg tcgaaattac gcggacatcg ctgaccggaa ctcccacggt
     4081 ttctgccacc atttgcgaga acacggtgtc ggcaccctgg ccgatttccg tcgcgccgct
     4141 ttgcacgttg atggttccat cctgattcat caacaggcgc gcgcctgcta tttctacgcc
     4201 gacaggccag gtgttagagg tgtagctaaa acaggcgacg ccaacgccac gacgtaaatt
     4261 gccttgctgg ttctggcact ctgcacggcg tttttcccat tcaaagattt tccggccttt
     4321 ttcaagacac tccggcaacc ctgcgctgta aatacgtttt cccgtgagcg gattagcatc
     4381 tccttcgcgg gcggcgttgc gtaaacgaat ttcaacagga tcaataccta acgctgtcgc
     4441 ggcatcatca agcatagact caacggcaaa tacgacttgt ggcgcgccat aaccacgcat
     4501 cgcaccagcc gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg
     4561 aggataaagg taagcgactt tattcccccc agcagaagca atggagtgcc cgtgagatgc
     4621 ataagcgccg gtgttagaca gaacatccag actataacct ttcaatgttc cgtcgcggtt
     4681 cacgcccatt tgcccgtcaa tagtaaaagc gtggcgggta cgggttgcga ggaaacactc
     4741 ttcacggcta agagaaactt tcaccggaat gccgccaagt ttgcttgtca ggaatgccgc
     4801 cattggctct tccagtacat cctgtttatt accaaaaccg ccaccgataa acggtttgat
     4861 gactcgtacg catgaccagg gaatatccag cgcctgacca accacgcggc gaacaatgtg
     4921 cgggatctgg gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc atgccagcga
     4981 tgtcacgctt tccatatgac aatgttgaat aacgggcgtc tgatagtgcc cctgtacctg
     5041 gtagtcggcg gcatcgattg tttgttggac attacccgtc gacatcgtgc tttgtttcag
     5101 taaattgcca ccgttatgga ttggtgcagc gtcttctgcc agcgccgctt ctgacgaggt
     5161 gataacgggt aattcttgcc actcaatgct gaccaattgc accgcttttt ctgccgtgag
     5221 ttcatcgcgg gctacgacga tggcaacggc gtcaccatga tgacgaacat gacgcgttag
     5281 cagggcacga tcggcagtat cgcgcttgtt ttcgtcaagt gtccaggcat gccctgccgt
     5341 ggcgaatggg atttctggca catcttccca ggtaaaaatc gccaggacgc ccggcaaact
     5401 cctggcttgt tcatcattaa tacttacggc ataaccatgt gcgatagggc tacgtacata
     5461 tttcgcgtaa cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac
     5521 cttagcaatg gcatcgacgc gcatgcatga ttcacccgta gcggttgctt cccgcgcttc
     5581 catagaaatc ccctcggttt gaacatttgg ttcttatggc aaagatttag tcttagcagg
     5641 caagaattac gccagaaacg actatatgcg tgaggggagg aaggaagtgt gatgacgtta
     5701 aaaaaacaca gatatgatac gaaacaggac tctgaatagg gacagtgata cgtatcaata
     5761 tgagtggcga taaaatcaat gtgataaatt aggattatta cgcatcaagg tgggaaatag
     5821 ggtaaaagtc aggaaattcg ataccactac agcaaatatt ttgctgatga tacttccctt
     5881 tgccaaatga aattaacctc tttctctatt acatcgtttt tccagaagca agagattggg
     5941 tatcaagagg ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga
     6001 gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat gttgttatcg gttggactgc
     6061 ttttggcggg ctgttcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt
     6121 acaccgtgaa acggggggat acgctatatc gtatttcgcg caccacggga accagcgtaa
     6181 aagaactggc gcgactgaac ggcatttccc ccccttacac cattgaagta ggtcagaaac
     6241 taaaactggg tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc aaatccacga
     6301 ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa atcttcctgg ccgccagtag
     6361 ggcaacgttg ttggttatgg ccaacgacag ggaaagttat catgccgtat tcgacagcag
     6421 atggcggcaa taaagggatt gatatctcag ctccacgagg tacacctatt tacgccgcgg
     6481 gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga
     6541 ttaaacacag tgaagattac attacggctt acgctcataa tgacacgatg ctggtaaata
     6601 atgggcaaag cgtgaaggct gggcaaaaaa tcgccactat ggggagcact gatgcggcat
     6661 ctgttcgcct gcatttccag attcgttacc gtgcaacggc aattgatccg ctacgttact
     6721 tgccgcctca gggcagcaag ccaaaatgct gatggcgaat taatcagcag tcagcagcgt
     6781 ggtacttgct aaggagagcg taaggtttat aatgccttac gcatctcgaa gcgggcgtag
     6841 ttcaatggta gaacgagagc ttcccaagct ctatacgagg gttcgattcc cttcgcccgc
     6901 tccaatttat cttcacatca tctacctgcc atttttgttg tatctgattt tctatattat
     6961 cttaattcaa gcgcttcaga cttttcgcaa tcgccgtaat acttccccat ttttttgtaa
     7021 ataaaaagcg tttacgcaat gcaatcgtca tgtatcttaa atagtcttat ttaagatgtt
     7081 cagaactctt tgtgtgttta actggctgat aattatataa tattacgaga gaagtgatag
     7141 agcggaataa taacaaatga acattatgaa aactgggatg gttcaaaatt gaagcaattt
     7201 tgaaccatcc acaacggata ttagctaaag aacattaccg atacgcacag gatacccacg
     7261 atcagggtaa tcagatttcc gatggaacgc cagggtttaa gcgccgggat gagatacgtt
     7321 gacagcgtag gcatgatgaa aagtatcatg gcaatgagcg ggccgctgat cgcgtaaatc
     7381 atcgaaatcg cgttcgggtt aatgcaacaa acaatgaagg taatcagcga taccaacata
     7441 attgatagtg cgcggttaaa tgcacgactt ttctttacac caacctgctg taatgttgtt
     7501 ttgacgacct ctgtggcacc ttcaataacg ccaaagtacg tacccaggaa tgatttcgac
     7561 atcgcaacta ctgcgacaat aatcccggaa attgacaacc atgctggggc gttcggcagc
     7621 atagaaagcg ccgataaaat ggtaacccct tcttctttag ccgcttcaat atacgaaggt
     7681 ggaatagaaa gcaggcagct aaagacaaag aacaatacac ttatgcagat gatgagataa
     7741 gcgactttca taattttttt gcatttatcc atagcatgtt cgccatattt ttcacgtctg
     7801 tctatggcaa acgtagaaat aatgggcgta tggctaaaag cgaaaaccat cacgggaatc
     7861 gatatccata tctggtgaag ggtattctga ttgaactcta cctgggttgt taatagatca
     7921 ggttgccaac taccgaccag gtaaatagaa agaaataaga aataggcaat caatgggaat
     7981 accaaaaatc ccattacccg aatagtggca tggcgtccca tcagaaaaat gagattcagg
     8041 attaatacaa cccccagact cactagcata cggatgcgaa gatcaataac catatgcttt
     8101 gccagctgtt ccgtaagtga gttggtaatt gccactgcat atatcaacac gacgacaaaa
     8161 aaggcgatga agtacagcgt ggtaatcaga ttaccaatct tcttgccata gtagtgtgtt
     8221 accgcgcccg ttatcccttc acctgctgat gttttcgatg agaggatgaa ctggcataag
     8281 gctttatgtg gccaatatgt taaaggccag gcgaccagag cagtaataaa caggaccaca
     8341 gcccccgccg aacctaactg aataggaagg aagagcgtgc ctgcaccaac ggctgtgccg
     8401 tagagtgcga aactccacag agtttcttct tttgaccaaa tattagacat tatctgaacg
     8461 tatcatctac aaattaaaca aaatggaggg caatttacca taaaccaggt taagtatggg
     8521 agattaacgc cttatctacc tctctattgg tgggttagtg gttgcaaacc ttacgtgcca
     8581 gttatgtctc tgactactac tcaggcgtat ttcctttgtg ataatggtca ctttt
//
LOCUS       DAJOTB010000182         8549 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJOTB010000182 DAJOTB010000000
VERSION     DAJOTB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.182"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            123..665
                     /gene="ecpR"
                     /locus_tag="OHN28_004259"
                     /gene_synonym="matA"
     CDS             123..665
                     /gene="ecpR"
                     /locus_tag="OHN28_004259"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCQ2299545.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            740..1327
                     /gene="ecpA"
                     /locus_tag="OHN28_004260"
                     /gene_synonym="matB"
     CDS             740..1327
                     /gene="ecpA"
                     /locus_tag="OHN28_004260"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCQ2299546.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1384..2052
                     /gene="ecpB"
                     /locus_tag="OHN28_004261"
                     /gene_synonym="yagY"
     CDS             1384..2052
                     /gene="ecpB"
                     /locus_tag="OHN28_004261"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCQ2299547.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2078..4603
                     /gene="ecpC"
                     /locus_tag="OHN28_004262"
                     /gene_synonym="yagX"
     CDS             2078..4603
                     /gene="ecpC"
                     /locus_tag="OHN28_004262"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HCQ2299548.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4593..6236
                     /gene="ecpD"
                     /locus_tag="OHN28_004263"
     CDS             4593..6236
                     /gene="ecpD"
                     /locus_tag="OHN28_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCQ2299549.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6205..6915
                     /gene="ecpE"
                     /locus_tag="OHN28_004264"
     CDS             6205..6915
                     /gene="ecpE"
                     /locus_tag="OHN28_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HCQ2299550.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7222..7551
                     /locus_tag="OHN28_004265"
     CDS             7222..7551
                     /locus_tag="OHN28_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2299551.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7563..7726)
                     /locus_tag="OHN28_004266"
                     /pseudo
     CDS             complement(7563..7726)
                     /locus_tag="OHN28_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7800..8414)
                     /locus_tag="OHN28_004267"
     CDS             complement(7800..8414)
                     /locus_tag="OHN28_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCQ2299552.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2276 a         2176 c         2156 g         1941 t
ORIGIN      
        1 gctacgtatc ttatttactt ccgatttact aaaaaaactg aatgtacctg taaaaattac
       61 aggtttggaa agtagtgaca tggcaaaatg attacagcag ggactatgag gttaaaaacc
      121 atatggaatg tcaaaaccgt tctgataaat acatctggtc tccccatgac gcctacttct
      181 ataaaggact atctgaactg attgtggata tcgacagatt aatttatcta tcgctggaga
      241 aaatcagaaa agatttcgtg tttatcaatc tcaatacgga ttctttaact gagtttataa
      301 accgtgataa tgagtggtta tccgcggtaa aggggaaaca ggtcgtattg attgcggcca
      361 gaaagtcaga agccttagca aattattggt attacaacag caatattagg ggcgtggtat
      421 acgctggact gagtcgtgat attagaaaag aactggccta tgtgattaat ggcaggttcc
      481 tgagaaaaga tattaagaaa gataaaatca ctgaccggga aatggaaatt atccgcatga
      541 cggctcaggg aatgctgcct aaatcgattg ccagaattga aaattgtagt gtgaagacag
      601 tgtataccca tcggcgtaat gcagaggcca agctgtactc aaaattatat aagttggttc
      661 agtaaactcc aggcaagtta gttttaaaaa atgactcacc gggacttcat gtcctcaatt
      721 caactcggga agaaaagcaa tgaaaaaaaa ggttctggca atagctctgg taacggtgtt
      781 taccggtaca ggtgtggcgc aggctgctga cgtaacagct caggctgtag cgacctggtc
      841 agcaacagcc aaaaaagaca ccaccagtaa gctggttgtg acgccactcg gtagcctggc
      901 gttccagtat gccgaaggca ttaaaggttt taactcacaa aaaggtctat ttgacgtggc
      961 tatcgagggt gactcaacgg ctaccgcctt taaactgacc tcacgtctta tcaccaacac
     1021 cttaacccag ttggatacct caggttccac actgaatgtg ggcgtggatt ataacggcgc
     1081 ggcagtcgaa aaaactggcg ataccgtgat gatcgatacc gccaacggcg tactgggcgg
     1141 caaccttagc ccactggcta acggttacaa tgccagcaat cgtaccactg cacaggatgg
     1201 tttcactttc tccatcatca gcggcaccac caatggtacc accgcagtaa ccgattacag
     1261 cactctaccg gaaggcatct ggagcggcga cgttagcgta cagttcgacg ctacctggac
     1321 cagttaatct ctctgatgta ccagcagggg tacccccctg ctttattcct ggacggaatt
     1381 gttatgaaaa agcaccttct gcctctcgct ctgctgtttt ccggaatatc accggcccag
     1441 gcgctggatg tcggcgatat atcatcgttt atgaacagtg acagcagcac gctgagcaaa
     1501 acgatccaaa acagtaccga cagtggccgc ctcatcaata tccgtctcga acggctctct
     1561 tcaccgcttg acgacgggca ggttatcgca atggacaagc cggatgagtt gctactcact
     1621 cccgccagct tgctgctacc cgcccaagcc agcgaagtga tccgcttctt ctataaggga
     1681 ccagcagatg aaaaagagcg ctactaccgc attgtctggt ttgatcaggc cctcagtgat
     1741 gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg cctccgcccg catcggcacc
     1801 atcctggtcg tcgcaccccg ccaggcaaac taccactttc agtacgccaa cggctccctg
     1861 acaaatacag gaaatgcgac gctgcggatc ctcgcctacg gcccctgcct gaaagccgcc
     1921 aatggtaagg agtgtaaaga gaattactac ctgatgccgg gcaagtcgcg tcgttttacc
     1981 cgcgtggaca cggcggataa caaaggacgg gttgcacttt ggcagggtga taagttcatt
     2041 cccgtgaaat agatagctgt gcagatggat aacgacaatg cctttacgac ggttctcccc
     2101 aggactgaaa gcccagtttg ccttcggcat ggtctttttg ttcgttcagc ccgatgccag
     2161 cgctgctgac ataagtgcgc agcaaatagg tggggtgatt attccgcagg ccttcagtca
     2221 ggcgctccag gacggcatga gcgtcccgct ttatattcat ctcgccggta gccagggtcg
     2281 ccaggacgat cagcgaatcg gcagcgcttt tatctggttg gatgatggac agttacgcat
     2341 ccgaaaaata cagctggaag agagtgaaga taacgccagt gtcagcgaac aaactcgaca
     2401 gcagctgatg actctggcca acgccccgtt caatgaggcc cttaccatcc ccctgactga
     2461 caacgcgcag ttggatctca gcttgcgcca actgctgctg cagctggtgg tcaagcgcga
     2521 agcgctgggc accgtactac gctcacgtag cgaagacatc gggcagtcca gtgttaacac
     2581 cctcagcagt aatctgagct ataacttcgg cgtctataac aaccagttgc gtaacggcgg
     2641 gagcaacacc tccagctatc tgtcgctgaa taacgttact gcgctgcgcg aacatcacgt
     2701 ggtgctcgac ggctcactgt acgggattgg tagcggtcaa caggacagtg aattatataa
     2761 agcgatgtat gaacgcgatt ttgctggtca ccgatttgcc ggtggaatgc tcgacacctg
     2821 gaacttgcag tccttagggc cgatgaccgc catttcagca gggaagattt acggcctttc
     2881 ctggggaaac caggccagct ccaccatctt cgacagcagc cagtcggcca cgccagtgat
     2941 cgccttttta ccggcggcgg gcgaagtaca tctcacccgt gatgggcggc tactaagcgt
     3001 tcagaacttc accatgggca atcatgaagt ggatacccgg ggtctaccgt acggtattta
     3061 cgatgtggaa gttgaggtaa tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt
     3121 caataagctg tttagccggg ggcgtggcgt cggtgcaccg ctggcgtggc agatatgggg
     3181 cggtagcttt catatggatc gctggtcgga aaacgggaaa aagacgcgac cagccaaaga
     3241 gagttggctg gcaggtgcct cgacctccgg ctcactgagt acgtttagct gggcggcaac
     3301 gggatatgga tacgataatc aggcggtggg tgaaacccgt ctgacgcttc cgcttggggg
     3361 ggcgatcaac gttaacttgc aaaacatgct ggccagtgac agctcatgga gcaacatcgc
     3421 cagcatcagc gccactctac ctggaggctt cagttcgctg tgggttaacc aggaaaaaac
     3481 ccgcattggc aatcaattgc gacgtagcga tgccgacaac cgtgcaatcg gcggcacact
     3541 caacctgaac tcactgtggt cgaagctggg tacgttcagc atcagctaca atgatgaccg
     3601 ccgctacaac agccactatt acacggcaga ttactatcaa agtgtctaca gcggtacctt
     3661 tggttcgctt ggcctgcggg ccggtattca gcgctataac aacggcgaca gcagcgccaa
     3721 tacagggaaa tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg
     3781 gatgacccat caaaacggct acaccatggc aaacctgtca gcacgcaaac agtttgatga
     3841 aggaaccatt cgcactgttg gtgccaatct gtcacgagcc atctccggcg ataccggtga
     3901 tgacaaaacc ctcagcggtg gggcgtatgc acagttcgac gctcgttacg ccagcggaac
     3961 gctgaacgtc aatagcgcgg cggacggcta catcaatact aatttgaccg ccaacggcag
     4021 cgtcggctgg cagggtaaaa acatcgctgc cagcgggcgg accgatggca acgctggggt
     4081 gatattcgac actggcctgg agaacgatgg tcagatcagc gccaaaatca acgggcggat
     4141 tttcccgctt aacggcaagc gtaactatct cccgctctct ccctatggaa gatatgaggt
     4201 ggagttgcag aacagcaaaa actcactcga cagttacgat atcgtcagcg gtcgcaaaag
     4261 tcatctgact ctctatccag gcaatgtcgc tgtcattgag ccagaggtga agcagatggt
     4321 taccgtctcc ggtcgtatcc gtgcggaaga cggcacactg ctggctaacg cacggattaa
     4381 caaccatatc ggccgaaccc gaaccgatga aaacggcgag tttgtcatgg acgtggataa
     4441 gaaatacccc actatcgatt ttcgctacag tggcaataaa acctgcgaag tggcactgga
     4501 actcaaccag gcgcgcggtg ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc
     4561 gtgggcggcg gtgacgcaga caggagaaga gaatgagagt taacctactg atagcgatga
     4621 taatctttgc gctaatctgg cctgcaaccg cgctcagagc ggcagtgagc aaaacaacct
     4681 gggcggatgc tccggcacgc gagtttgtgt ttgtcgaaaa caactcagac gacaactttt
     4741 tcgtcactcc tggcggggcg ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt
     4801 taaaatacaa tggttcagga accatctatc agcaaagcct cggctacatt gataacggtt
     4861 acaacaccgg cctttatacc aactggaagt ttgatatgtg gctggaaaat tcaccagttt
     4921 catctccttt aactggcttg cgctgcatca actggtacgc agggtgtaat atgaccacca
     4981 gtcttatcct gccgcaaacc accgacgcca gtggatttta tggcgcgacg gtgaccagcg
     5041 gcggcgcgaa gtggatgcac ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa
     5101 tgcccgtcgg cagcagcttt acaatgacca tcaatgcctg ccagacctct gtgaactatg
     5161 acgccagcag cggcgcacgc tgtaaggatc aggcctccgg caactggtat gttcgcaacg
     5221 tcacccatac gaaagcagca aatctgcggt tgataaatac ccactcgctg gcggaagtat
     5281 ttatcaacag cgacggagta ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa
     5341 ccatcggcag ccgttcagga ttaagttgta agatggttaa ctataccctg caaacaaacg
     5401 gactcagcaa cacctcaatc catatattcc cggcgatcgc caactcgtcg ttagcctcgg
     5461 ccgtcggggc gtacgatatg cagttcagtc tgaatggcag ttcatggaaa ccggtgagca
     5521 atactgccta ttactacacc ttcaacgaga tgaagagcgc agactcgatc tatgtgttct
     5581 tctcgagcaa cttctttaag cagatggtga acctcgggat cagcgatatc aacaccaaag
     5641 atctgttcaa ctttcgcttt cagaacacca catcaccgga gtctggctgg tatgaatttt
     5701 ctacctccaa cacgctgatt atcaaacccc gtgatttcag catcagtatt atctccgatg
     5761 aatatactca gacaccgtcg cgggagggat atgtcggcag cggcgagtcg gcactcgatt
     5821 tcggctatat cgtaaccacc agcggtaaaa cagctgccga cgaagtgctg atcaaggtga
     5881 ccggacccgc gcaggtgatt ggcgggcgct cctattgtgt cttcagctcc gatgacggta
     5941 aggcgaaagt accgttcccg gcgacgcttt cctttattac ccgcaacgga gctacaaaaa
     6001 cctacgatgc cgggtgcgat gatagctggc gggatatgac cgatgcgctg tggttgacca
     6061 caccgtggac tgatatctct ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt
     6121 cgattccaat ggataacgcc atttctctgc gtacggtaga tgataacggc tggtttggcg
     6181 aagtcagcgc ttcaggagag attcatgttc aggcgacgtg gcgtaacatt aactaaggcc
     6241 ctgctgacag tggtctgtat gctggcggca cctttgacac aggcgatttc ggtcggcaat
     6301 ctgacttttt cactgccgtc cgagactgac tttgtcagca aacgtgtcgt gaataacaac
     6361 aaaagcgcac ggatataccg tattgccatc agtgctattg atagcccggg cagcagtgaa
     6421 ttgcgcaccc gaccggtgga tggtgaacta cttttcgccc cccgccagct ggcgttgcag
     6481 gctggtgaga gcgagtattt taaattttac tatcatggcc cacgggataa ccgcgagcgc
     6541 tactaccggg tctcatttcg cgaggtcccc actcgtaacc tgacaaaacg cagcccaagc
     6601 ggcggtgagg tcagcacgga gccggtggtg gtgatggata ccattctggt agtacgacca
     6661 cgtcaggttc agtttaaatg gtccttcgat caggtgacag gaacggtaag taacaccggc
     6721 aacacgtggt ttaagctact gattaaacca ggatgtgatt cgaccgaaga ggaaggcgat
     6781 gcctggtatc tacgtccggg agacgtggtt catcagcctg agttacgtca gccggggaat
     6841 cattatctgg tctataacga caaattcatt aagattagcg attcctgtcc ggctaagccc
     6901 ccttcggcgg actaagactt atccatcgga cgagggaaga attccacctc agagactcaa
     6961 aaatcgttta aatgatgtgg aagcgatcgt caatgtcacc gatgagcttt ctcatatgca
     7021 caccacagtg ttcaggagac acggaaaggt accaggcggc ctgctaacga gtggtttctt
     7081 cactaattag gacgtaaaat cggtaacggc tggaaatcat tcaatactca cactatcgaa
     7141 agtacaccag ccaaccgcga tacgtccctg catacgacgt accgcagtta tctttacgat
     7201 ttatagctgt actggtgaat tatgagcaat ttgaatccat gcatgacgtg tggtgcctgt
     7261 tgtgcatttt tccgcgtctc tttttactgg gccgaagccg acgatgctgg cggtaccatt
     7321 cccgccaggc tcactgaaca aatatcccct tttcaccgat gcatgagcgg tactaatcag
     7381 aaaaatcccc gatgtattgc ccttgcagga acaccgggca aaaatgcctg ctgcacgata
     7441 tatgaaaatc gatcgtccac atgcagagaa ttcgccatgt ctggtgagaa cggagaagtc
     7501 aatgaggctt gcaatcgtgc aagagctaaa tacgggctaa caccgttata aatatacaat
     7561 aattaattgc attgccccgc cagctgtaat agcggggctt cgtttttcag ggacaacaaa
     7621 acctgaaact tcttccattt tccagcaaca atgcctcttc ttctgtagcg tcctccggat
     7681 ctacatcatc ctgaagtagc gcgatatgat cactgactgt ccgcatcaga atatgaaaag
     7741 caacgttttt aacctataaa aatggcgcta tatttgcgcc atttttatca ttcaaagcat
     7801 tatctatttg agcctaaagg gatctcagga tctggctcat gggtaattct gtttcgtaaa
     7861 tctctgcgaa taatttcaat agaccagaac cagactaaat gtccaaaaat ttcagagaca
     7921 ttctcatacc acggaagatc aaagagaggt ggcgtaagtc ccatgagagg gaatgaaatc
     7981 atatgaacaa aaagttgcgc taaagcacct gccagtaagc cctgccaaag tttaattttc
     8041 ggaaatactt cggcgaccac acagtaacca acggcaaaaa ctatcgaaaa gattatgtgc
     8101 gttacaccaa cccagttaaa gacatgcccg gcgaaggtat aaacagccga atttggatct
     8161 gtcagtccca accaatcccg aagaaaaatg tacggaggat tgagaaagtt acgggagcaa
     8221 tcaatttggc ctgcagccct gattaatgat tccggaccac acgctgcatt aaacatatca
     8281 accggactac gtggtggcaa tggaacttct gccccccact taacaaatgc ggaaacaacg
     8341 ccagcaatca gcccaatgaa tgcagcaaga ccataacgtc tgcggtttgg tggagtttgt
     8401 tcaaatatat tcatatctac cctgcttgta ccattatgtt atacacctct ttaggagtat
     8461 tcataaaaca aggcaaatgt aaagaactgt attgttttgt ataaccacat ggttacctaa
     8521 tcgccaatta atataagctc cattatttc
//
LOCUS       DAJOTB010000183         8511 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJOTB010000183 DAJOTB010000000
VERSION     DAJOTB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.183"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..560)
                     /locus_tag="OHN28_004268"
     CDS             complement(<1..560)
                     /locus_tag="OHN28_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299553.1"
                     /translation="MNKNESISYTEMQKFFAREIKEVGGRPITSKNLELSLLPIHARE
                     NLSAPEMPWYTEVNDIYMEFRRSPFWMVAGYVFLGFMLSLLSISILSIVAFSFYEMGF
                     NSDLLFLYLLAFVLGCFILPGAAKIAFFTPHYLPIRFNRKTQKIYVSDLIIEGNIFFG
                     KIKLTFHEYDWKDVEGWEVSRHFSKS"
     gene            850..1092
                     /locus_tag="OHN28_004269"
     CDS             850..1092
                     /locus_tag="OHN28_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299554.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYLRKLREKW"
     gene            complement(1274..2203)
                     /gene="fdhE"
                     /locus_tag="OHN28_004270"
     CDS             complement(1274..2203)
                     /gene="fdhE"
                     /locus_tag="OHN28_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCQ2299555.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2200..2835)
                     /gene="fdoI"
                     /locus_tag="OHN28_004271"
     CDS             complement(2200..2835)
                     /gene="fdoI"
                     /locus_tag="OHN28_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2299556.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2832..3734)
                     /gene="fdoH"
                     /locus_tag="OHN28_004272"
     CDS             complement(2832..3734)
                     /gene="fdoH"
                     /locus_tag="OHN28_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCQ2299557.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3747..6797)
                     /gene="fdnG"
                     /locus_tag="OHN28_004273"
     CDS             complement(3747..6797)
                     /gene="fdnG"
                     /locus_tag="OHN28_004273"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6210..6212),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2299558.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6991..7824
                     /gene="fdhD"
                     /locus_tag="OHN28_004274"
     CDS             6991..7824
                     /gene="fdhD"
                     /locus_tag="OHN28_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCQ2299559.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7906..8466
                     /locus_tag="OHN28_004275"
                     /pseudo
     CDS             7906..8466
                     /locus_tag="OHN28_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         2038 a         2150 c         2195 g         2128 t
ORIGIN      
        1 atacttttac tgaaatgtct actcacctcc caaccttcaa catctttcca gtcatattca
       61 tgaaaagtga gttttatttt tccaaagaag atattgcctt caataattaa atctgaaaca
      121 taaatttttt gtgtttttct gttaaatctt ataggaagat aatgtggcgt gaaaaaagca
      181 atttttgcag cgccaggtaa tataaaacag ccaagcacaa atgcaagtag atacagaaat
      241 aacaagtcac tattaaagcc catctcataa aatgagaatg caacaataga taatatcgag
      301 atggaaagta gtgaaagcat aaatcccagg aatacataac cggcaaccat ccagaatggt
      361 gagcgtctga actccatata gatatcatta acttcggtat accacggcat ttctggcgca
      421 gaaaggtttt ccctggcgtg aatgggtagc agacttaact ctaaattctt agaagtgatg
      481 ggcctaccgc ctacttcctt aatttctctg gcaaaaaatt tttgcatttc ggtgtaagaa
      541 attgattcgt ttttattcat tatatctgtt ccgttccctg acgacttatc gttttttctt
      601 ggtagatgac attaagctat tggatgtgag aaaggcaact ttatagagta aattcagaaa
      661 gagtagaaat tttcacacga ctgttttcac ttccattttt tactggtttt cgtaataact
      721 gcaactgtta catcgtatct ggaaaacgcc tcgcaaaaat aaaaagtgat gcgtaaatgc
      781 gctttcttta aagattcgac aagggtactt ttctctccat agattcaaaa tgatattggc
      841 gagatattta tgaaccctct ggcaggtatg gatatgagca gaattttact cgatttatcg
      901 aatgaggtga ttaagcaact tgatgatctt gaggtgcagc gtaatcttcc gcgtgcagac
      961 ctattacggg aagcggtaga tcaatacctg gtaaatcaat cgcaaacagc aagaaccagt
     1021 gttcctggca tctggcaagg gtgtgaggaa gatggtgtcg aatacctgcg taagctgcgc
     1081 gagaaatggt aatggcaaaa agatttggct atttgatatc gatatcctta tcgattttct
     1141 cagcgtgaaa attgaggcca aacaggcact ggcggcatag cttgaggagg taacgcggaa
     1201 tacgagagat agtgtcgtca caccctatga actgtaatcc gggcgacctg cgccacccgg
     1261 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactacggg
     1321 catagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg
     1381 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1441 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1501 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcagtttca cacaggttgc
     1561 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1621 agccacatac cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga
     1681 tcagattagc catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat
     1741 cgctgctgac ggacgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1801 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1861 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1921 gaatatccag cgggggcttg ccttgtgcgc tggcttcttt aatgcgcgtg gtcagatcca
     1981 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     2041 gcaggtaatc acccagcgga ttatgttctg ccagctcgcg cagacgctcg gcgcggcggt
     2101 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     2161 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     2221 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc tttgcccatg cgctggtcac
     2281 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2341 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2401 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2461 caacaggacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2521 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2581 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2641 aaacataacc acgccgacaa acggatgcag aattcgcgcc agctgcggtg tgcccatgat
     2701 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     2761 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2821 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2881 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2941 aagccaacag ctgcgagtgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     3001 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagtacgtac
     3061 ataacgtgtg taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg
     3121 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     3181 tgaatcgcgc ccgttgggca ggtcttcacg caggccggtt cttgcccaac caccacgcgg
     3241 tcaacgcaca acgtacattt gtagacgcgg ttgtcttccg ggttgaggcg tggcacgttg
     3301 aacgggcacc ctgcgatgca ataaccgcag ccgatgcact gctcggactg gaagtcgacg
     3361 ataccattgg catactgaat aattgcccct tccgccgggc atgctttcag gcagcctgga
     3421 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3481 acttccgaga atcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3541 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3601 caggctttac agccgataca ggtggtaacg tcgatgagtt tcgccacttc ttcctggaag
     3661 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc
     3721 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3781 aactccggcg tctgcgtgtt cgcatcaccg acgaatggcg tcaacgtatt ggcaataaag
     3841 ccttttttcg caacaccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3901 ttgccgtttg ctttcagcgt gcgaatacgt ttggtcacta ccgctttggc tttgatatag
     3961 ccacggttgg aggagacttt cacagtatcg ccctgggcaa tgccaagttt attcgccagc
     4021 gactccccga tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg
     4081 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     4141 gctttcccca atgcttcggc atcgtcttta aagatacgcg ccgccggatt cgagataacg
     4201 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     4261 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4321 aacggcccga cgccactgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4381 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4441 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4501 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttct
     4561 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcagt
     4621 tgagcgaacg agctaagttg ttggcctttc ttgacaataa ccgcaccggt tgccggatcg
     4681 gtaatatcgg ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca
     4741 tgcgggatgg cgtagttcca ggtcatgttc agcacctggt ccgggttcgc gccaccctgt
     4801 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc
     4861 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc agagttaacg
     4921 attgaaccgt tctcttccgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4981 ttcgacgagt caacttcgtt cagttcaccg tggttctgcc agaagttaga ggtttcagtg
     5041 ttcagcgggt cgatagtcac gaggaatttc agcttcgaca gacagccgat cactttgttt
     5101 ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc
     5161 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     5221 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     5281 acgaagaatt tcgggtagtt gccccagtag ttaacctggc cttccagcag tggtttcggc
     5341 gtgttggcgg taaggtaagt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5401 cctggcagac tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5461 cgcagggcgt taacgccgcc gccagccatc cccatattgc cgagcagcaa ctggatcatc
     5521 gccatcgtac ggatgttctg cgcaccgatg gagtgttgcg tccagccgag ggcgtaaagg
     5581 aacgaggcag ttttatcgtg agcactggtc tctgcgatgt attcgcagac tttcaggaat
     5641 gcgtcttttg gcgtaccgca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5701 tgtttcagca agttccacac gcaacgtgga tgttgcaggg tagtatcgcg tttggcgaag
     5761 ccgttttcgt ccagttcgta agtccaggtg gacttatcgt acttgcgttt ttccgcgtcg
     5821 tagccggtga acaggccatc ttcaaagccg taatcctcac gcacgatcag gctagcgttg
     5881 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5941 cctgacagga aagcaatgtc agtaccggaa cggatagggg catagtagtc agccaccgcc
     6001 gctgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     6061 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     6121 acgaggttgg cattcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     6181 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gactgcgagc
     6241 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttgct cgacgcggaa
     6301 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6361 atgtagtttg catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6421 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6481 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc tccgcggttg
     6541 accgggtgat ccgggtcacc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6601 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6661 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6721 aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6781 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6841 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6901 cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6961 gtgtgtgtgt tcttatctgg ttaatagaaa gtgaaaaaaa cacagcaaaa agaaatcgag
     7021 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     7081 cacccacggt tagatgaggt ggcggaagaa gttcccgttg cgctggtcta caacggcatt
     7141 tcgcacgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     7201 ctttccgaag gaattatcga aagcccgcgc gatatcttcg gcatggatgt cgttccttcc
     7261 tgtaatggcc tggaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggaa
     7321 cgtcgccggg cgctggcggg acgtacgggc tgtggcgtat gcggtgtaga acagcttaac
     7381 gatatcggta aaccggtgca gccgctgccg ttcacccaga cgtttgatct caacaaactg
     7441 gatgatgcat tacgtcatct caatgatttc cagccagtgg ggcaactgac tggttgtact
     7501 cacgccgctg catggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7561 cgccatgtgg cgctggacaa actgttaggc cgccgatcac aagaagggga aagctggcag
     7621 caaggtgcgg tgctggtttc cagccgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7681 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cacttgctgt agaagtggct
     7741 gagcgctgta atctgacgct ggtgggcttt tgtaaaccgg gtcgggcgac ggtttatacc
     7801 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattacagg
     7861 acttattcat ttcgtgaatt ttgttatttt tttataaaca aaacgcgtgt agtggcgtta
     7921 atgcctgcgc tgcaacctat tgatggcgtg gcggtgagtt atattgatgc tgccgtagcg
     7981 ttgggtaata ccattaacga aatggataaa tattacaccc aggaaaatta taaagacgat
     8041 gcgtttgcga aagggaaaac attccactac tactctctgg cagtcatgat ttcagcgaaa
     8101 caaattaata acctgatatc gcaagataag tttgatgcag aagcggcaat gaagaaagtg
     8161 tctgaactgg aaacgctggt ggcgcaagcc aaagaagcgg ataaaagcgg catgaatttc
     8221 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8281 agagataaag tcccgtactc tgactgggat aaagagcaac ttcaggacgc aaactcaagc
     8341 tggatggccg aagactcatt tccacgagcg ttatgcgatt acaacgaaat ggtcgatgaa
     8401 atatttcagt taattgtaat cgcggggcgg gtttgtgatg agcacggata tgtcacgaag
     8461 tcgtaacaat ataaagcaga gtgaaggata aattttgtga ataaagtatc a
//
LOCUS       DAJOTB010000184         8499 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJOTB010000184 DAJOTB010000000
VERSION     DAJOTB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.184"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            135..740
                     /gene="azoR"
                     /locus_tag="OHN28_004276"
     CDS             135..740
                     /gene="azoR"
                     /locus_tag="OHN28_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCQ2299560.1"
                     /translation="MSKVLVLESSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(780..1646)
                     /gene="pdxI"
                     /locus_tag="OHN28_004277"
     CDS             complement(780..1646)
                     /gene="pdxI"
                     /locus_tag="OHN28_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HCQ2299561.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(1866..>8499)
                     /locus_tag="OHN28_004278"
     CDS             complement(1866..>8499)
                     /locus_tag="OHN28_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HCQ2299562.1"
                     /translation="HGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNA
                     ISNGGTGTQINGDEATVNNNGNTTVDGQGSTGTEIAGNNVVVNQDGTLDVSGGGHGID
                     ITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVN
                     NNGNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTD
                     PDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIA
                     GNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNND
                     GDNAISNGGTGTQINGDDATANNNGKTIVDGKDSTGTEIAGNNAVVNQDGTLDVSGGG
                     HGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDE
                     ATVNNNGKTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGM
                     TVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQINGDDATANNSGKTIVDGKDSTG
                     TEIAGNNAVVNQDGTLDVSGGGHGIDITGDSAKVDNKGGMTVTDPDSIGIQIDGDKAI
                     VNNDGDNAISNGGTGTQINGDEATVNNNGNTTVDGKDATGTEIAGNNAVVNQDGTLDV
                     SGGAHGIDITGDGAIANTTGAITVADTDSVGIQVDGNAVTVSNDGDSTITNGGTGTQV
                     NGNDAQVNNTGNTTVDGKNSTGTEITGNNGTVNLDGGLIVTGGAYGVEIAGDSAALNN
                     EGDISVSDEGSIGVLINGEKATVSNTGDVNVSNNATGFDITTNEGNISLAGSMQVGDF
                     STGMDLNGNNNSVTLAAKDLNVTGQKATGVNVSGDANTINITGNVLVDKDQTADNAAD
                     YFYDPSVGINVNGSDNNVTLDGKLTVVADSELTTRGSAGAIIFDGSQENITGLSIAGD
                     GNTFRLNGGIQLVGEANKLTDGSTIASERKGSGKVPLVSVDGKSSVHLNGDSTISGDV
                     LLGNTQIIDLRNGAMLDVGADATINMQVDTYERYAMSADHLIDASSGSQLVNNGDINL
                     RSIGFAAITGEDSFGSNTGNLTLSQYLDGLFANGGYGYLATKGASVVNTGTITTKVAE
                     QESVINLGASLGFNDLVFYNAANSMTGLSAKTHGYVLNENGGKIEMFGRGNVGMLAID
                     ESTAENAGQITLDALWVDVNDTTTLRDNIGNDARGYSVGMGVGTDASSGPRKNATAVN
                     QQSGVITVYNAGIGMAAYGAGNTVINQGTINLEKNGNYDSSLGVNSLIGMAAYNSGTA
                     INDQTGVININADNGQAFYSTGNGVILNYGTICVNTNCLTGNDYNDTDSYTSILYTGG
                     DVITAQSETQSLTKKATISDKKDGNVVNSGALSGADIAISSGKLVNTSTGTINNAIII
                     NGGALSNEGSVAKVTQNAGTFNNTGSVNSRMTQTGGTFNNQQGGTVKNGSRLANSAIA
                     NNMGIWSLGDTSSGSNASMLEINNNAVFKNSGDFILDNSKNAVHLNQSGTFYNTGKML
                     LSNSSHSGVLNFWGGNGRFINGGIADVTAKSLAVSASDAGSSNAFFWNQSNGVVNFDK
                     DSGVAVKFIHSNYVAQNDGTMNISGNNAIAMEGDKNARLVNNGTINLGTSGTTDTGMI
                     GMQLDSGTTADAVIENNGAINIYANNSFAFSMLGSVGHLVNNGTVTIADGVTGSGLIK
                     QGDSVNIEGVNGNNGNNSEVHYADYTLPDVPGSSVSVAASTPSSDGSQNKLNGYVVGT
                     SSDGSAGKLKVNNASLKGVSVNTGFTAGTSSTSVTFDNVVQGSNLTDAETITSTSVVW
                     NAQGTTDASGNVDVTMTKNAYTDVATDSSVNTVAQVLDAGYTNNELYSSLNVGTTAEL
                     NSALKQVSGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNDT
                     QYDMMALRKSLTLTEHQNLSLEYGIARLEGNGSDTAGDNGVTGGYSQFFGLKHQMAFD
                     NGMNWNNALRYDVHQLDSSRSVAYGDINKTADANVKQQYLEFRSEGAKTFELREGLNV
                     TPYAGVKLRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKDGWSANATL
                     EGGPNLSYVKSQRTASVSGAGSQRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAF
                     HWKEDGISDKGVMLNFKKTF"
BASE COUNT         1824 a         2373 c         2032 g         2270 t
ORIGIN      
        1 gcagctttct atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct
       61 ctgtggcagg attgtgaata aagtgtcaac aagcaacggg gcattgccca ttcaaatatc
      121 tataaggaaa caccatgagc aaggtattag ttcttgaatc cagcatcctg gcagggtact
      181 ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg
      241 atgaaatcac cgtgcgtgac ctggctgcaa atccgattcc ggtcctggat ggtgaactgg
      301 ttggtgcact gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc
      361 tttccgatga gctgattacc gagctgaaag cccacgacgt tatcgttatt gcggcaccga
      421 tgtataactt caatatctcg acccagttga aaaactattt tgacctggtt gcacgtgcag
      481 gcgttacttt ccgctatacc gagaagggtc cggaaggcct ggtcaccggt aaaaaagcca
      541 tcgttatcac cagccgcggt gggatccata aagatggccc aacggatctg gtaacgccat
      601 atctgtccac attcctgggc tttatcggca ttaccgatgt gaaatttgtc ttcgccgaag
      661 ggatcgcgta cggcccggaa atggcagcga aagcacagtc tgacgcaaaa gcagccatcg
      721 acagtattgt tgctgcataa gatttatgat gcctcccacg aaagtgggag gttttttatt
      781 taacccgcct tttgcgcaat actgtccagc gttgccagca cgtcatccgt tagcactaat
      841 tcatcggacg ccatattctc ccgcagatgc cccaccgaag aggtgccagg aatcagcaaa
      901 atattcggtg aacgctgtaa cagccacgcc agcgccacct gcattggtgt cgcattaagg
      961 cttgtagcaa catcggaaag cgtggaggat tgcagcggcg taaagccccc gagcgggaag
     1021 aacggcacgt aggcaatgcc atcacgagcc aaagcatcaa tcattgcgtc atcagcacgg
     1081 tgggcaatgt tgtattcgtt ctgcacgcag acgatttcgg caatctttcg cgcctctgca
     1141 acctgcgtcg gtgtgacgtt actcaggcca agatgtttta ccagaccttg ttgttgcatc
     1201 tccgccagca ctgtcaggct ggcctcaatc gatccttccg ctgggccatg accatccccc
     1261 atcataacgc gcaggttaac cacatccagt acgtccaggc cgagattacg tagattatcg
     1321 tgtaccgctt tttgcagctc tgccggagaa aatgcgggta accaggatgc gtcctctcca
     1381 cgccgcgcac caattttagt gacaattgtc aggtcgtcag agtaaggata aagcgcttcg
     1441 cggataatct gattggtgac gtgcggacca taaaagtcgc tggtatcaat atgattgacg
     1501 cccaatgcca gcgcctcgcg cagcacggtt atagcgacgt ggcgatctct tggggggcca
     1561 aaaaccccag gaccagccag ttgcatcgcg ccataaccaa gacggttaac ggattttgta
     1621 ccgagagtaa atgtgttgct gctcatcgca ccctgccctg taataaaacg gattaagaaa
     1681 aatcagtata gaaacgaaaa gagataaata atgcgagggt gtaaacaaga ttgcgggatg
     1741 agttgtactg ttgccctgaa agacacgaaa ccccaggcag gttaagacac gtcaagcacc
     1801 gcatccggca ataaaaaaac cgcaacctgt atcagcgttg cggtttatgc attacatgct
     1861 aaaaattaga acgttttctt gaagtttagc atcacacctt tatcactgat gccgtcttct
     1921 ttccagtgga atgcatctac ctgcaacgca ctttcctggc tgctgtattt cacacccatt
     1981 gtcgccaggc tgttgaaacc accaccattc tgaccatcat caatgttgaa acgctggctt
     2041 cccgcgcccg aaacgctcgc agttcgctgg cttttcacat agctcaggtt cgggccgcct
     2101 tccagcgtgg cgttcgcact ccagccgtcc ttaccggcat agtccagttt cagcccaaca
     2161 atgctgtcca ctgccgtttc gctaccgctg ttcatgctca ggttaaagtc acccgcattg
     2221 cgttcctgat agccaccctc cagtgtgtga cgcagtttaa ccccggcata tggcgtgacg
     2281 ttcagccctt cacgcagctc aaaggttttc gccccttcac tacggaactc caggtactgt
     2341 tgtttcacat tagcatcggc tgttttattg atatcgccgt acgctaccga gcggctgctg
     2401 tcgagctggt gcacatcata acgcagggcg ttattccagt tcataccgtt gtcaaaggcc
     2461 atctggtgtt tcagaccgaa gaactggctg taaccgcctg tcactccgtt atcgcctgcg
     2521 gtatctgaac cattaccctc cagacgcgca atcccgtatt ccagactcag gttctgatgt
     2581 tcagtcagcg tcagagattt acgcagcgcc atcatgtcgt actgtgtgtc gttacccagt
     2641 tcagcacgcg ggtcgccttt cgctaccacg ttgaaggcca gaccgtttgc gacttccggt
     2701 gctgcatcgg acaacatgct gaagcggtta cttaacactc gtgcttcatt gaataccgtt
     2761 gttgcctggc tgccgctgac ctgtttcagc gcactgttca gttccgccgt ggtgcctacg
     2821 ttcaggctgc tgtacaactc gttgttggta tagcccgcat ccagtacctg cgcgacggtg
     2881 ttaacagatg aatctgttgc tacatcggtg taggcatttt tagtcatcgt gacatcaacg
     2941 ttgccgctgg cgtcagtagt gccttgcgcg ttccatacca cagaggtcga tgtaatcgtc
     3001 tctgcgtcgg tcagattact gccctgaacg acgttgtcga aggtcacgga ggtggagctg
     3061 gttccagccg taaatccggt gttaacagaa acccctttca ggcttgcatt gttcactttc
     3121 agcttgccgg cactgccgtc agaactggtt cctacaacat aaccattcag tttgttctgg
     3181 cttccgtcac tggacggggt gctggccgca acagaaaccg acgagccagg tacatcaggc
     3241 aaagtgtaat cagcataatg cacttcgcta ttgttaccgt tattgccgtt cacaccttca
     3301 atgttaacgc tgtcaccttg cttgattagc ccggaacctg tcactccatc ggcaatagtc
     3361 actgtgccgt tgttcacgag gtggcctacg gaacccaaca tgctaaatgc aaaggagttg
     3421 ttggcgtaaa tgttgatggc gccattgttc tcaatgaccg catcagctgt cgtgccgctg
     3481 tccagttgca tcccgatcat gcccgtgtcg gtcgtgcccg acgttccgag gttgatcgtg
     3541 ccgttgttaa ccagtcgtgc gtttttatcc ccttccatcg caatagcgtt attaccgcta
     3601 atattcatgg tgccgtcatt ctgcgcaacg taattgctgt gaatgaattt cactgcaacg
     3661 ccgctgtctt tatcaaaatt gacgacccca ttgctctggt tccagaagaa ggcattgctg
     3721 ctcccggcat cactggcgct tacggccaaa gatttcgccg tgacatccgc aataccgccg
     3781 ttaataaagc gcccattccc accccagaaa tttagtacgc cgctatggga agagttggat
     3841 agcaacattt tcccggtatt gtagaacgta cctgactgat tcagatgaac agcgtttttg
     3901 ctattatcca gaatgaagtc accactgttt ttgaacacgg cattattatt aatttccagc
     3961 atgctggcat tactaccact actcgtatcg ccaagggacc agatccccat gttattagca
     4021 atagcgctgt tggccagtct ggagccgttc ttcaccgtcc cgccctgttg gttattgaac
     4081 gttcctcctg tctgagtcat acgactgtta accgaccctg tgttattgaa agtaccggcg
     4141 ttttgcgtca ccttcgccac agagccttcg ttacttaacg caccgccgtt gataattatt
     4201 gcgttattaa tcgtacccgt tgatgtatta actaacttac cgctactaat cgcaatatca
     4261 gcaccggaaa gcgcaccact gttaacaaca ttaccgtctt ttttgtcgct aatcgtggct
     4321 tttttggtca gactttgtgt ttcactctgt gcagtgatga catcgccacc cgtgtataat
     4381 atcgaggtat aggagtcagt atcattatag tcattgcccg tcaggcaatt agtgttaaca
     4441 caaatggtgc catagttaag aatgacaccg ttaccagtgc tataaaatgc ctgcccattg
     4501 tcagcattaa tattgataac accggtttga tcgttaatgg cggtaccact gttatatgca
     4561 gccataccga taagcgaatt cacgcccagg ctactatcgt aattaccgtt tttctccaga
     4621 ttaatggtgc cctggttaat aaccgtatta ccagcgccgt aggcggccat accaattccg
     4681 gcgttataaa cggtgataac accactctgt tggttgactg ctgtggcgtt cttcctcggt
     4741 ccactgctag catctgtccc aacgcccata ccaacagaat atcctctggc gtcgttacca
     4801 atgttgtcac gcaaagttgt tgtatcgttt acatcaaccc acagtgcatc cagtgtaatc
     4861 tgccccgcgt tttctgccgt actttcatcg atggccaaca taccgacatt gccacgtccg
     4921 aacatctcga ttttgccgcc gttctcatta agtacataac catgagtttt ggcgctaagg
     4981 cccgtcatac tgttcgctgc gttataaaag actaaatcat taaaacccag tgacgctcca
     5041 aggttgatta cgctctcttg ttccgccact ttcgtagtga tagtgcctgt gttcacgaca
     5101 ctggcccctt tggtggctaa atagccatac cctccgtttg caaacagccc atcgaggtac
     5161 tgagaaagcg tgagattgcc agtgttgctt ccgaatgaat cctcaccagt aatagcggca
     5221 aaaccgatgc ttcggagatt aatatcaccg ttattgacca actgagagcc agacgatgca
     5281 tcaatgaggt gatcggctga catagcataa cgctcgtaag tatcaacctg catgttgatc
     5341 gtggcatccg caccgacgtc cagcatggca ccattccgca aatcaatgat ttgtgtattg
     5401 ccgagcagca catcaccgct aatagtggaa tccccgttca gatgaacgga cgattttcca
     5461 tcaacactga caagaggtac ctttccactg cctttccgct cggatgcaat agtggaaccg
     5521 tcagttagct tgttcgcctc accaaccagt tgaataccgc cattcagacg gaaagtatta
     5581 ccatcacctg caatgctgag tccggtgata ttctcctggc tgccatcaaa tataatggcg
     5641 ccagcagaac cgcgtgtagt aagttcactg tcagcaacaa ccgttaactt accgtccaga
     5701 gtgacattgt tatcactacc gttgacatta atccccacag acgggtcgta gaaataatct
     5761 gccgcgttat cagccgtttg atctttatct accagaacat taccggtgat attaatagtg
     5821 ttggcatcgc cggatacatt cacgcctgtc gccttctgac cggtgacgtt cagatctttt
     5881 gccgccagcg tgacgctgtt gttattaccg tttagatcca tcccggtaga gaagtcgccc
     5941 acctgcatac tgcctgccag cgaaatattc ccttcattag tggtaatgtc aaaacctgtc
     6001 gcgttattgc tgacgttaac atcacccgtg ttggatacgg ttgctttttc accgttgatg
     6061 agtacgccaa ttgagccctc atcggaaacc gaaatatctc cttcattgtt tagcgcggcg
     6121 ctgtcgccag cgatctccac gccataggca ccacccgtca caatcagacc accatccaga
     6181 tttacagtgc cgttattgcc cgtaatttct gttccggtgg agtttttgcc gtctaccgtg
     6241 gtgttgccag tattgttgac ctgagcatcg ttgccgttaa cctgcgtgcc cgtaccgccg
     6301 ttagtaatgg tgctgtctcc gtcattgctg acggtcactg cattaccatc aacctgaata
     6361 ccgacagagt ccgtatccgc tacagtgatc gctcctgtcg tgtttgcaat ggcgccgtct
     6421 ccagtgatat caatcccgtg agcaccgccg ctgacatcca gcgtgccgtc ctgattcacc
     6481 accgcgttgt tacccgcgat ttccgtgccg gtggcgtctt tgccatcaac agtggtgtta
     6541 ccgttattat tcacggtggc ttcatcgccg ttaatttgcg tgccggtgcc gccgttgctg
     6601 atagcattgt cgccatcgtt gttgacgatg gctttatcac cgtcgatctg gataccgatg
     6661 gaatccggat cggtaaccgt cataccgcct ttgttatcca ctttggcgct gtcgccggtg
     6721 atatcaatac cgtgaccgcc gccgctgaca tccagcgtgc cgtcctgatt caccaccgca
     6781 ttgttgcccg cgatttcggt accggtggaa tctttgccgt cgacaatggt tttgccgctg
     6841 ttgttcgcgg tcgcgtcgtc accgttgatt tgtgtgccgg taccgccgtt gctgatggcg
     6901 ttgtcgccgt cgttgttgac gatggcttta tcaccgtcga tcagaatacc gatggaatcc
     6961 ggatcggtaa ccgtcatacc gcccttgttg tccaccgtgg cgctgtcgcc ggtgatatca
     7021 ataccgtgac cgccgccgct gacgtccagc gtaccgtcct gattcaccac cgcgttgtta
     7081 cccgcgattt ccgtgccggt cgagccctga ccatcaacgg tggttttacc gttattgttt
     7141 acggtggctt catcaccgtt aacctgcgtg ccggtgccgc cgttactgat ggcgttgtca
     7201 ccgtcgttgt tgacgatggc tttgtcgccg tcgatcagaa taccgatgga atccggatcg
     7261 gtaaccgtca taccgccctt gttgtccacc gtggcgctgt cgccggtgat atcaataccg
     7321 tgaccgccgc cgctgacgtc cagcgtaccg tcctgattca ctacagcgtt attacccgcg
     7381 atttccgtgc cggtggaatc tttgccgtcg acaatggttt tgccgttgtt gttcgcggtc
     7441 gcatcgtcac cgttaatttg tgtgccggtg ccgccgttgc tgatggcgtt gtcgccgtca
     7501 ttgttgacga tggctttatc accgtcgatc agaataccga tggaatccgg atcggtaacc
     7561 gtcataccgc ccttgttgtc caccgtggcg ctgtcgccgg tgatatcaat cccgtgaccg
     7621 ccgccgctga cgtccagcgt gccgtcctga ttcactacag cgttattacc cgcgatttcc
     7681 gtgccggtcg agccctgacc atcaacggtg gttttaccgt tattgttcac ggtggcttca
     7741 tcaccgttaa cctgcgtgcc ggtgccgccg ttgctgatgg cgttgtcgcc gtcgttgttg
     7801 acgatggctt tgtcgccgtc gatcagaata ccgatggagt ccggatcggt aaccgtcata
     7861 ccgcctttgt tatccaccgt ggcgctgtcg ccggtgatat caatcccgtg accgccgccg
     7921 ctgacatcca gcgtgccgtc ctgattcact acagcgttat tacccgcgat ttcggtaccg
     7981 gtcgagccct gaccatcaac ggtggtgttg ccgttattgt ttacagtggc ttcatcaccg
     8041 ttaacctgcg tgccggtgcc gccgttgctg atggcgttat cgccgtcatt gttgacgatg
     8101 gctttgtcgc cgtcgatcag aataccgatg gaatccggat cggtaaccgt cataccgccc
     8161 ttgttgtcca ccgtggcgct gtcgccggtg atatcaatac cgtgaccgcc gccgctgaca
     8221 tccagcgtac cgtcctgatt caccaccacg ttgttacccg cgatttcggt acctgtagag
     8281 ccctgaccat caacggtggt gttaccgtta ttgtttacag tggcttcatc gccattaatc
     8341 tgcgtgccgg tgccgccgtt gctgatggca ttgtcgccgt cgttgttgac gatggcttta
     8401 tcaccgtcga tcagaatacc aatggaatcc ggatcggtaa ccgtcatacc gcccttgttg
     8461 tccaccgtgg cgctgtcgcc ggtgatatca attccgtga
//
LOCUS       DAJOTB010000185         8496 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJOTB010000185 DAJOTB010000000
VERSION     DAJOTB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.185"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            277..1389
                     /locus_tag="OHN28_004279"
                     /pseudo
     CDS             277..1389
                     /locus_tag="OHN28_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582666.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1448..1783)
                     /locus_tag="OHN28_004280"
     CDS             complement(1448..1783)
                     /locus_tag="OHN28_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCQ2299563.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(1787..2131)
                     /locus_tag="OHN28_004281"
     CDS             complement(1787..2131)
                     /locus_tag="OHN28_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCQ2299564.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(2133..2306)
                     /locus_tag="OHN28_004282"
     CDS             complement(2133..2306)
                     /locus_tag="OHN28_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299565.1"
                     /translation="MSYFSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            2407..2591
                     /locus_tag="OHN28_004283"
                     /pseudo
     CDS             2407..2591
                     /locus_tag="OHN28_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2552..2671
                     /locus_tag="OHN28_004284"
     CDS             2552..2671
                     /locus_tag="OHN28_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCQ2299566.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            2706..3048
                     /locus_tag="OHN28_004285"
                     /pseudo
     CDS             2706..3048
                     /locus_tag="OHN28_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3415..3681)
                     /gene="minE"
                     /locus_tag="OHN28_004286"
     CDS             complement(3415..3681)
                     /gene="minE"
                     /locus_tag="OHN28_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCQ2299567.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3685..4497)
                     /gene="minD"
                     /locus_tag="OHN28_004287"
     CDS             complement(3685..4497)
                     /gene="minD"
                     /locus_tag="OHN28_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCQ2299568.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(4521..5216)
                     /gene="minC"
                     /locus_tag="OHN28_004288"
     CDS             complement(4521..5216)
                     /gene="minC"
                     /locus_tag="OHN28_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCQ2299569.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5736..6104
                     /locus_tag="OHN28_004289"
     CDS             5736..6104
                     /locus_tag="OHN28_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299570.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(6207..6608)
                     /locus_tag="OHN28_004290"
     CDS             complement(6207..6608)
                     /locus_tag="OHN28_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCQ2299571.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            6849..7142
                     /locus_tag="OHN28_004291"
     CDS             6849..7142
                     /locus_tag="OHN28_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCQ2299572.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            7214..7873
                     /locus_tag="OHN28_004292"
     CDS             7214..7873
                     /locus_tag="OHN28_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCQ2299573.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            7965..8411
                     /locus_tag="OHN28_004293"
     CDS             7965..8411
                     /locus_tag="OHN28_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCQ2299574.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         2260 a         1849 c         2035 g         2352 t
ORIGIN      
        1 gaaatcaaaa ataatacttc tacaagtaat ggagagtatc taatcactta agacgaaagc
       61 catgctgtga aagtggatga cggtgtaact ggatatataa ataatgccag tgtgatgact
      121 agtggtgatg gatcttatgg tatttctgtt gatagccaaa acaaagtatt atatataagc
      181 gatagcaata ttaagacctc tggaagcgta tctgacaaag aaaatggagg gataacagcc
      241 agcgcagtag tcagtgaatt tggtggcacc atcgttatga atggtgataa ttcagtcgag
      301 acgagggggc atattcagcg ggatttttaa gtcaggttaa tgattctggg actgtggaaa
      361 ataacaccag tcttgaaacc acagataaaa cgaacattgt tacctatggg gaaaatgcag
      421 taggagttct tgcatgttca agtcctggag agtctcgaac atgtgtcgat gctgtagatg
      481 atgaagtttg tgattctaac agttacgaag ttattagccg tgctgattta aaaatgaatg
      541 gtggttccat aacaactaat ggtattaata gctatggtac ttatgctaat gggaataaag
      601 catatattaa tttagattat gtggtacttg aaactgtggc tgatggaagt tatgcagttg
      661 ctattcgaca aggtaacatt gatataaaaa agttctatta caacaaatgg cactaaagcc
      721 cccattgcaa aaatatacaa tggtagagag tgattttttt ccaatgtcac ttcagaatca
      781 acagaaacgc ctacccccgt cctgcgaccc gacgctggca gttacgtcgc caacctggca
      841 gccgctaaca ctctttttgt tacgcgtctg aacgatcgtg cgggtgaaac gcgctatatc
      901 gatcctgtaa ctgaacagga gcgttcaagc caactttggc tatgtcaaat tggcgggcat
      961 aatgcctggc gtgacagcaa cggacagttg agaacgacct cggcacaggc catctggatg
     1021 ggcgtcgatc ataatgcgca cacggaagcc aatggctcac gtattgagaa tgacgcaaat
     1081 tacaacatcc agagccgact tggtttccgc acctttattc gtactcagga gaaaaacagc
     1141 ggtccgcacg gtgacgactt tgaacctttt gttgaaatga acgggatcca taacagtaaa
     1201 gattttgctg tctcaatgaa cggtgtgaaa gtcgaacaag acggggctcg taatctgggg
     1261 gaaatgaaac atggcgtaaa tggcaatctg aatccggcgg ccagcgtctg gggtaatgtg
     1321 ggcgtgcagc tgggtgataa tggctacaat gacaccgcaa tgatggtggg cctgaaatat
     1381 aagttctgat cccgcccgtt agctaaaaaa ccgcgtcgaa ttcatcgacg cggtaacata
     1441 tgaaatatta ttttttgccg atagcacgca tggtgtcatc aattgccgtg atcaacagca
     1501 tttgcggatc tttagcgcaa acctgattca gtttttctac cactttggcg ctcagttccg
     1561 gagattgctc aatttttaaa tcacggctgg caacgctggc attacccatt accgcaacaa
     1621 tttctgcaac ctgtgcgctg tcagtttttg ccatttcgtt ggcttctgcg caagtaatat
     1681 aggtttctga tggcaaaccg ttcttaatat tgtagtcctg cgcccaggtc attggtgcga
     1741 aaacaaacag acccgccagt aaagcaaatt ttttcatcat cattccttat ttcattttac
     1801 ccagaattgc accacccgta ccgccaatca cggcaccttt aatcgcccct tcgaggccat
     1861 tgccggtcag aacgccagtg acagcaccaa cggcggcacc cacttttgca cctttacgcg
     1921 catttttacc gtcacggcct ttttctgtta ctgcaccaac accagcgcca acagctgcac
     1981 ctttcagtac gccattaaca ccattgccag taagtaaacc aacgcctgca cctagcaatg
     2041 cacctttcgt ggtgcgattc atatccgcca ttgctggcgt ggagcagaac aatgctgaga
     2101 taagcccgaa ggcgagtatt tttttcttca acttagttgt ccggtattaa gtaagttgca
     2161 cacacaataa tttcgtcttc aattaagatc tgcttaacta aagaacgctc gctattattc
     2221 agataattca aaatgagcgt gactgtgatg ataggaatta tgttttttac gggaatgaga
     2281 ataatctcaa atgagaaata actcattgat tgacaatatt tttattcaag aagtgtcatt
     2341 ggctgttaat acaatgttgt aaaggtaaga taatctgatt tatcaatatt attgtgtgat
     2401 tgttatgtga gcagaagata ttcattagca acgattacat tagtcatttt attttgtgga
     2461 cggcctcatt gtcgaaaaat aagcgtacga cagtattatc agaaaagagt gttttttatc
     2521 caacaacact tcagcgcact gcgtttaaaa aatgcctctt tcttatacgg gatatcatca
     2581 tttcatcatg atgtctttga tgagcggtga acacaataca tttgcgctgt ctcttcagga
     2641 tgaatcccct ggtctggtgc ctgcgggctg atgttgcagc agagctaagg tcacttagac
     2701 gctactatca tttatccaat gcatggaatc gaaatcagtc gatacccgca gtatatatcg
     2761 tgaactgggc gcaacgctga gctacaacat gcgtctgggg aacggtatgg aaattgaacc
     2821 ctggctgaag gcggctgtgc gcaaagaatt tgtcgatgat aaccgggtga aggtgaatag
     2881 tgacggtaat tttgtcaatg atttgtcggg cagacgtgga atataccagg caggtattaa
     2941 agcctcattc agcagtatgt taagcgggca tctcggggtg ggtataaccg tggtgccggt
     3001 gtggagtccc cgtggaatgc ggtggccggt gtgaactggc cgttctgacc atcaacgatt
     3061 aactgcgttt cggcgctgtt ttcgtttaca gggtgctaaa agggaaaatt ctggggcaaa
     3121 aaaagcccgc cagttacggc gggaaacctc atcctattgg agaacaatga ataatgaaat
     3181 tgcgggttat catctccctg gatacccatg ctaacaataa ggttatttac tcaaccaggc
     3241 gtaaacattt tgttctgtgc gtgggaacag ccttaaggtg taaagggggg ggaaatagca
     3301 atgagtagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     3361 aactcaaggc agagatgact ctgccttgaa gataaatgcg cttttacagg gggcttattt
     3421 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     3481 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     3541 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     3601 agcaacaata atctgcaacc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     3661 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     3721 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     3781 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     3841 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     3901 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     3961 cagcaggtgc tctttaatcg gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     4021 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     4081 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     4141 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     4201 acgggtgagg gcatctttat cgcgagtttg cgatgccgga agaatataga gattttcagt
     4261 acgtttatct ttaattaacg cctggtttag cgttgcatcg ccctgaatga cgttgacgaa
     4321 atcgtaaacg acccggcgtt cacaacccat aatcaggtcg agattacgca ggccgatatc
     4381 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     4441 ggaggttgtc ttaccaacac cccctttgcc cgaagtaaca acaataatgc gtgccataga
     4501 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     4561 tgcagtcgtg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     4621 cctgcgatgg acaccagttc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     4681 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttaccatcg
     4741 gcaatcaatt cggcccctgc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     4801 taaatacgct gaccggaacg cactggggta tcaattaaac gcgtttttgt gaccggcgtt
     4861 gtattttgcg ctggagcctg cggtgcgggg gctggacgcg gcgatttttc ctttccttcc
     4921 gtcaggatag gcagccccat cttttcaatt tcggctttaa gttgcgcatc tttgcagcca
     4981 cttacgccaa taacccgcaa accagttgcc gaaaccgcct tatgcatcgc cgaccagttt
     5041 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     5101 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     5161 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     5221 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     5281 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     5341 tctattctgt gataaatggc gttgattcat agcttaaaaa atacccttgt caatcaaccc
     5401 attgccgtcg tacttttgat tgttcttatt tacgcttcct ttttccgcac cctaatcgtg
     5461 acagcatctt cagacctgtt taaatatcca gtattgaggc gagtgtattt tttcaccacg
     5521 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     5581 taatatccgc cgcagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     5641 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga tgaaatgagc
     5701 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attttttata
     5761 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggaaat gacgctggcg
     5821 ggcttcaatc tcctgcgtgt ggcgttgttt gcgacccgta tatttgtgtg agttcagatg
     5881 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     5941 cattacaagg ctacgatccc agcgaattta cattctctaa cggtgtattt tgcgatgtta
     6001 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtgga tggtaagcgg agtggaaaaa
     6061 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgtctt gtttacatta
     6121 tgttctattc gtcattaacc tcttaagcga cagagaggag tgtggcgtta acgtttatga
     6181 cggggagagt ccccgtcggt tgacatttac ttaatttgaa tatcgacgtt atatttttta
     6241 gttttgtttt tacgggcgtc gtttcgggtt tgcagcactc gcagctcata tttgccagaa
     6301 gcagggagtg agtattgtcc gtgactatcg agttctgggg aatatctgga taaatcaaca
     6361 gaatcatcga ttcctggccc gaagaggtaa gtatcagcgc cttcattaga gatactcaca
     6421 tgcactttct gacctttttt ggcatagaag gtatatgtat cgtaatcgta tccctttatt
     6481 tcgccggagt actgagcgct gctatggcct tttctgaact caacattgac attcttaccc
     6541 gcagcaaagc tcgtggaagt tagcagtgca agcaacaata cagccttact gatgccctta
     6601 attttcattg gtagtctcct gttcagaatg agtacgatga actgttataa tataacaatc
     6661 cttcacggga gaattccagt ggctttcctg tgttcggtta aatcctggcg cggcattttt
     6721 gccgggggat tacttataaa gggggcatgg actgaacaaa ccgtagaaat ctacagctgg
     6781 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
     6841 gtaaaagtat gttttgtgtg atttatcgaa gcagcaaacg tgaccagacc tatttatatg
     6901 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
     6961 ctcagttagc gatgattctt ccgctggatg ggcggaagaa actggtcaat gccgatattg
     7021 aaaaggttaa acaggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
     7081 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     7141 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     7201 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     7261 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     7321 agtacccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     7381 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     7441 gattggcgcg acgctgcgtc aggctacgga agagcatgtc cgcaaagcca ttgccggtta
     7501 cggcgtggcg ctcgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     7561 gccgtgggaa aaggccaaag cgtttgataa ctcttgtccg ctttccgggt ttattcccgc
     7621 ggcggaattt accggcgacc cgcaaaatac aacgctgggc ctgagcgtaa acggcgagca
     7681 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgctga tcgcttatat
     7741 gagcaagttt tttaccctca aggtcggtga cgttgtgctg acaggcacgc ctgatggtgt
     7801 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     7861 tcgcgttttg taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc
     7921 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     7981 tctggcaaag taaaaccctg gacgaaatga gcgatgcaga atgggagtcg ttgtgtgatg
     8041 gttgcggtca gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca
     8101 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     8161 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     8221 ggctgccaat gacctgcgct tatcgtttgc tggcggaagg taaaggttta cctgcgtggc
     8281 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     8341 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     8401 gggcgcagtg ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatgc
     8461 tcgccatttc gtagccctgt ttttgaaagc gctctg
//
LOCUS       DAJOTB010000186         8477 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJOTB010000186 DAJOTB010000000
VERSION     DAJOTB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.186"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            162..2540
                     /gene="ppsA"
                     /locus_tag="OHN28_004294"
     CDS             162..2540
                     /gene="ppsA"
                     /locus_tag="OHN28_004294"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCQ2299575.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2597..4243)
                     /gene="fadK"
                     /locus_tag="OHN28_004295"
     CDS             complement(2597..4243)
                     /gene="fadK"
                     /locus_tag="OHN28_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCQ2299576.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4300..4593)
                     /locus_tag="OHN28_004296"
     CDS             complement(4300..4593)
                     /locus_tag="OHN28_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2299577.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4590..5879)
                     /locus_tag="OHN28_004297"
     CDS             complement(4590..5879)
                     /locus_tag="OHN28_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2299578.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5934..6872)
                     /locus_tag="OHN28_004298"
     CDS             complement(5934..6872)
                     /locus_tag="OHN28_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCQ2299579.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(6892..7656)
                     /locus_tag="OHN28_004299"
     CDS             complement(6892..7656)
                     /locus_tag="OHN28_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCQ2299580.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7972..>8477
                     /locus_tag="OHN28_004300"
     CDS             7972..>8477
                     /locus_tag="OHN28_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCQ2299581.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         2026 a         2187 c         2195 g         2069 t
ORIGIN      
        1 cgaagagagc agatttgcgc aacgctggga tcagtcttaa aaagtaaaaa aatatatttg
       61 cttgaacgat tcaccgtttt tttcatctgg ttaaatatgc aaagataaat gcgcagaaat
      121 gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac aatggctcgt
      181 caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg gttgggggca
      241 aaaatgcctc cctgggtgaa atgattacta acctttccgg aatgggtgtt tccgttccga
      301 atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc ggcgtaaacc
      361 agcgcattta tgaactgctg gataaaacgg atattgacga tgttacccag cttgcgaaag
      421 cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag ctggaaaacg
      481 ccatccgcga agcctatgca cagctttccg ccgatgacga aaacgcttcg tttgcggtgc
      541 gttcctccgc tactgcagaa gatatgccgg acgcttcttt tgccggtcag caggaaactt
      601 tcctcaacgt tcagggtgtt gacgccgttc tcgtggcagt gaagcatgta ttcgcttctc
      661 tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgaccat cgtggcgtag
      721 cgctctccgc cggtgttcag cggatggtgc gctccgacct cgcatcttct ggcgtgatgt
      781 tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc gcatggggcc
      841 ttggtgaaat ggtcgtgcag ggtgcggtta acccggatga gttttacgtc cataaaccga
      901 cacttgcggc gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa aaaatccgca
      961 tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac gtaccgcagg
     1021 aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca aaacaggccg
     1081 tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa gatggtcaca
     1141 ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc ggtcaggtca
     1201 tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt gctatcggtc
     1261 atcgcatcgg cgcgggtccg gtgaaagtta tccatgacat cagtgaaatg aaccgcatcg
     1321 aacctggcga cgtgctggtt actgacatga ccgacccgga ctgggaaccg atcatgaaga
     1381 aagcatctgc catcgtcacc aatcgtggcg gtcgtacctg tcacgcggcg atcatcgctc
     1441 gtgaactcgg cattccggcg gtagtgggct gtggtgatgc aacagaacgg atgaaagacg
     1501 gcgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat gcggagttgc
     1561 tggaatttag cgttaaaagc tccagcgtag aaacgatgcc ggacctgccg ttgaaggtga
     1621 tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc ctgcctgccg aacgaaggcg
     1681 tgggccttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac ccacgcgcac
     1741 tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag atgatgaaag
     1801 gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc gcgacgctgg
     1861 gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa tcgaacgaat
     1921 atgccaatct ggtcggtggt gagcgttacg agccagatga agagaacccg atgctcggct
     1981 tccgtggcgc gggccgctat gtttccgaca gcttccgcga ctgcttcgcg ctggagtgtg
     2041 aagcagtgaa acgtgtgcgc aacgacatgg ggctgactaa cgttgagatc atgatcccgt
     2101 tcgtgcgtac cgttgatcag gcgaaagcgg tggttgagga actggcgcgt caggggctga
     2161 aacgtggtga gaacgggctg aaaatcatca tgatgtgtga aattccgtcc aacgccttgc
     2221 tggcagagca gttcctggaa tatttcgacg gcttctcaat tggctcaaac gacatgacgc
     2281 agctggcgct cggtctggat cgtgactccg gcgtggtgtc tgaactgttc gatgagcgca
     2341 acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc cgcgaagaaa cagggcaaat
     2401 atgtcgggat ttgcggtcag ggtccatccg accacgaaga ctttgctgca tggttgatgg
     2461 aagaggggat cgatagcctg tctctgaacc cggacaccgt ggtgcaaacc tggttaagcc
     2521 tggctgaact gaagaaataa aataaatacc cggcggcgtt ttgtcgccgg gtttatgtga
     2581 tccccgaaga tgaaacttat tcaatctctt cacagacatc ctgagttaat cgccgcataa
     2641 tatctttcct taacaaaaac ttttgtattt tacctgagac agtgcgcggt agtttttcga
     2701 ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta aaaaaagcca
     2761 ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa gcgcatgaac
     2821 gttcacctaa acgttcatcg ggcatcgcaa ccacacaggc atcgtgaatt ttaggatgct
     2881 gcaataaaat atcttctact tcacggctgc taatattttc gccgccgcgg acaataatat
     2941 ctttcttgcg cccggttatt tttatatagc cagcctcatc catgcggcag agatcgccgc
     3001 tgtaatacca gccttcttca tccagcgcac gggcggttaa ttcaggttca tcaaaatacc
     3061 ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc ggtaaagtct
     3121 tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaaccg tcggtgtgca
     3181 taaagcgcgg caaaggatcg tcgagattca ccaccgcatg cggcgaactt tctgtggaac
     3241 cataaacact taataattta atgccgcgct gctggcattc tcgcgccact tttttgggga
     3301 ttgtggtacc tccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt tgtttctcta
     3361 gtaaattcaa aagatcatag acaaacggcg ttgcgccgag catacaggtg caacgctgct
     3421 gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg ctgcgagcac
     3481 caatcaaaaa aggtgctgtt acgccatgca gaaagcccgt tgcgtgacca agtggcgcag
     3541 gcatcataaa gacatcctgc caggtcagat ttagccgcgc gcaataagcc cgctcactgg
     3601 cgagaatatt gttatgcgtt agcatcacac cctttggcag accctcggtt ccggaggtaa
     3661 atagcacagc ggctaattca tcgccgtggg tcgttatcgc cgtggtcaga ggagtattgt
     3721 cggcgagaat ctgacttaat gagagggaag aggtggcggg agccagtttg tccacgccga
     3781 caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct accggacgcg
     3841 tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttattg agcacccaca
     3901 ccagttctgc ttcccgccag gaaggtaaca gcggcacgga aaccgcaccg gttttcaggc
     3961 aggcaagata gataacggta aattcacacc agcctggcag ttgaaatgcg atgcgatcgc
     4021 ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg tgatcgagcg
     4081 cgctataggt gtacgttgca ccatgattat cgaccacggc aattttgtct ggcatcgcac
     4141 gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccataaccct tgctgacgat
     4201 acgccgcacg acgttgttcg ttgaacgtta atgtcacttt cattttggac tctcgaaaca
     4261 gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc gaaattcaat
     4321 accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttgccgc acagcacccg
     4381 acaggtgccg cactccagac aaccggcgga atcgaaatga atgtttcctg cgtcatcctg
     4441 cttataaagt ccggcagggc aagctttcat taatttacgg aattcattga tatcgggatt
     4501 tgccgccaaa atgatatgcg ggtggccctc atcaacatgg aatttatcga cgcctaattt
     4561 gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt aatgccatct
     4621 ttcagcaagt tgatcagccc aattttcttc gcgtgtccca tgatcatttt gcgtaccggc
     4681 tggtttggtt tgccgtcaat ggtgaacata tcgttcatga tgtcggcgac cattcgtggg
     4741 tattggctaa acaggcgcgg gttttccatc agcgccggga tcttgcggaa atgctgcata
     4801 tcgcgcataa cacaactttg ttccagctca cgtttgtatt gcgccagact gctggcggag
     4861 aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc tgatgcaatg
     4921 gctaaatcca tgccgcgaac tgtaaaaccc aaattcaggc agaagcctgc ggcgtcacca
     4981 acgatcatca cgccatcgtt aaccagctgc ggcaccattg ccagaccgcc ctccggcacc
     5041 atatgcgcgg aatattcaag cagtttgccg ccgctaatca gcgggcgaat agcggggtgt
     5101 tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat atcacccaat
     5161 ccacaaacca gccccaacga tacggaatcc ttgttggtat agaggaatcc gccgcccatc
     5221 aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc cgtaacatta
     5281 aagcgatcgt tgatctgttc tggtgtcagg cctataacct ctttaacacc aacagcgtaa
     5341 tgatgcggat tggaagcgga aaccattccc agcgagcggc caagcatcga gttaacgcca
     5401 tcagccagaa tcaccacatt cgcttcgaga atatcatccc cggcctggac gccagtgacc
     5461 ttgtttcctt cacgaaccag cgcatcgacg cgtacgcccg ggataaactg cgcgcccgcc
     5521 tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt ataagacgcg
     5581 tgttgcggaa catctggttg ctcgcggtga aaatcgaggg taacggcgct ttcttcggtt
     5641 aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatgc tgcaaagcct
     5701 ggaataattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt tttacatccg
     5761 gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgcgccat gaccagtgcg
     5821 gcaacgctac cggcaacgcc cgcaccgacc acaatggcat caaatttgtc atccgacatg
     5881 cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg gaattagcga
     5941 ttcaactggc taatcagggc agggacgact ttgtagatat cgcccaccag accgtagtcg
     6001 gcatagttga agattggcgc atttttatct ttattaatgg cgacaatcac ttttgcgccg
     6061 ttgccgccaa ccatatgctg gatctgcccg gagatcccca gcgtcaggta gagatcggat
     6121 ttcagcaaca cgccggagac accgatataa cgttcacgct ccatccagtt ctcgccttcg
     6181 gcaattggac gtgaacagcc gacttcagca ttcagcactg ccgccagttc gtggaccatt
     6241 ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa ccacacgttt tgctttgctc
     6301 aggtccacgc tgcttgccgc tttcgcacgg cgttcgcgac agagaatttc atgacgcggg
     6361 gcaacataag gtaccgtttc tgtcgggcac tgatgagaga tatcacgggt gcacggttcc
     6421 tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt tgatcttttc ctgagcgaac
     6481 gctaacccgc catacatccg gtgttcggcg caaatgtgac catcgacaat atccaccgcc
     6541 gtggcatcgt tcaccagcgc cgcattcagt tgcacactta accgtgcggc cagagcttta
     6601 ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt tatctttcag cagggcagca
     6661 atgctttcgg cgtaattttc agtacgttgc agcgcgtcgt tttgctcaag aacataaatg
     6721 cattttggcc cataaggcat aactgcctgc gcctgctcgg tattttgtac aatggcatac
     6781 acctgttggc cccattgctg agcgctgcca aacagttcag cataacgttc aggattatcg
     6841 ctaaagaccc agacgctgtt taattgactc atcgtttctt ctccgtaggc ttcagttcag
     6901 ggctttcttc agatgttccg ccagctcggc aatggcctcc ggcgaatcgt tatcgagaat
     6961 gatgtgctta cgttctgttt gcggcggtac gcgaatgccg gtcagttcag caagtggtgc
     7021 gctctggctc cagccaatat cacttgcctg ccactgattt accggttttt tacccgcgcc
     7081 gagaatggct ttcatcgaag gaatacgtgg cacgttaata tcggaggtga cgcagagcac
     7141 ggctggaaca gagagttcaa taacttcaac atcatcttca agcgtgcgtt caatcaccag
     7201 tgtattgccc tgacgctgaa tagcactcac tgcattaatc accggaagtt gcagaatttc
     7261 tccgaccagc aagccaacct gctgggcata aaggtcgccg gaaccttcac caaagatcag
     7321 taaatcgaag ccgatctttt caactgctgt cgccagcgcc tttgcggtat cgagaggcag
     7381 tgcatgctca agttgtgcat cctgcaccat atacaggctg tgcggcccgc gggatagcac
     7441 gtctttgcgc actttcgagt tctgcaacaa agagccgccg acggtcagcg cggctatctc
     7501 atcgtcatct gttgcaagct ggcttgcagc ttcaatggca ttgagatcga actggctgat
     7561 tttggcgtcg gcattgtcga aattcagggt gtattctgga gtgacaacaa tgtcctgttc
     7621 ttcaggcacc agcttaaagc aggttattat tttcatgatt tctcctgtga gtcgcatacg
     7681 gaaccatctc cgatggaaag taaaaatggc ctgaaaatag tgtgcgatag cggtgcgggg
     7741 ttttcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat gcgatcgcta
     7801 aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtactatc ttgcattcag
     7861 tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat acacgccgta
     7921 ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt catgtatcaa
     7981 cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc acggctttca
     8041 cgtttcgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca tgacaatgaa
     8101 accgagctga tttacgtcaa gaaaggggta gcaaggttta ccatcgattc ttcgttatat
     8161 gtcgcgcatg ccgatgacat tgtggtgata gaacgtggca ggttgcatgc ggtggcctct
     8221 gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca gtttcagggg
     8281 gctgaggaaa atcagctact gcaaccgcat tcttgcccgg taattgccgc aggacaggga
     8341 aaagaagtca ttaaaacctt atttaatgag ctaagtgtga ttttgccgca aagtaaaaat
     8401 agccaaacat cttcgttatg ggacgcattt gcctatacgt tagcaattct ttactacgaa
     8461 aactttaaaa atgctta
//
LOCUS       DAJOTB010000187         8404 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJOTB010000187 DAJOTB010000000
VERSION     DAJOTB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.187"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(103..567)
                     /locus_tag="OHN28_004301"
     CDS             complement(103..567)
                     /locus_tag="OHN28_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCQ2299582.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGIAEKEQSFIFYTLISWLQEKGGLHK"
     gene            complement(560..991)
                     /locus_tag="OHN28_004302"
     CDS             complement(560..991)
                     /locus_tag="OHN28_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2299583.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTNGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            complement(1087..1515)
                     /locus_tag="OHN28_004303"
     CDS             complement(1087..1515)
                     /locus_tag="OHN28_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCQ2299584.1"
                     /translation="MTRALAVVVALALVALGWQSWRLNSASHTIETQRAALKSKAQEL
                     TKKNSQLIGLSILAETNNREQARLYAEAEQTSALLRQRQRRIEELKRENEDLHRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGCAAH"
     gene            complement(1512..2027)
                     /locus_tag="OHN28_004304"
     CDS             complement(1512..2027)
                     /locus_tag="OHN28_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2299585.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVIRVEQALERCVKQQPPQK
                     VYDAVVSFAFNVGTGNACSSTLVKMLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAQ
                     EMAWCLKGAGL"
     gene            complement(2008..2223)
                     /locus_tag="OHN28_004305"
     CDS             complement(2008..2223)
                     /locus_tag="OHN28_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299586.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2227..2430)
                     /locus_tag="OHN28_004306"
     CDS             complement(2227..2430)
                     /locus_tag="OHN28_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCQ2299587.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2430..2894)
                     /locus_tag="OHN28_004307"
     CDS             complement(2430..2894)
                     /locus_tag="OHN28_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCQ2299588.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYVSLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAESSGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(2990..3640)
                     /locus_tag="OHN28_004308"
     CDS             complement(2990..3640)
                     /locus_tag="OHN28_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCQ2299589.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLTAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3644..4699)
                     /locus_tag="OHN28_004309"
     CDS             complement(3644..4699)
                     /locus_tag="OHN28_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCQ2299590.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKDQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAEAGA"
     gene            complement(4716..5549)
                     /locus_tag="OHN28_004310"
     CDS             complement(4716..5549)
                     /locus_tag="OHN28_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCQ2299591.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFADVHKAVELVVTEQQNLSAR
                     TEKSLSEQEERLSELETALQEQQTAFNELVNKLSHEDSRQDYRQRVTGGNAPADTLTN
                     C"
     gene            5692..7458
                     /locus_tag="OHN28_004311"
     CDS             5692..7458
                     /locus_tag="OHN28_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCQ2299592.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7458..>8404
                     /locus_tag="OHN28_004312"
     CDS             7458..>8404
                     /locus_tag="OHN28_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2299593.1"
                     /translation="MGKSKKNRAAATKQIQLKSQTTAEAFSFGDPVPVLDRRELLDYV
                     ECVQMDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGIQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASNVFVRNEL"
BASE COUNT         1786 a         2484 c         2221 g         1913 t
ORIGIN      
        1 aattttgggg cgcataacat cctatcctta agaaaatcat atcattataa caaatacttt
       61 tatatatagc agattttata ttttcattga agtaaaataa actcacttat gcaaccctcc
      121 cttttcctgt aaccaactga ttaatgtgta aaatataaaa ctttgttctt tttcagcaat
      181 gcctaacaaa cgacgctctg cataacggac ctccggtcct tttcgactga cgcgatcacg
      241 caggccgtaa tggtgaacgc gggcgatgcg ctgcacctta ccttcaaact gtacgctggc
      301 agaatccgcg ctggcggcgg ttttcaggta ttttgtggtg cgaagttttg taaacatctg
      361 ccgtttgatg cggccttttt tactgcgtgc tgttactcgc ctcggttcat agctgctgcc
      421 atcaggattg cgttgcatcc tgatattctg ctgctgtgtc cggcgcagtt cctgcgccag
      481 ctggcgcatc atgcggcttc tggcggctgg ttccagattc gccagcaagg cactcagcca
      541 gtcgtccact ttctgcaatt cagccacgtt tcaccgtcca catttcttca ggttcatcag
      601 gttccgctat agcttcaacg ctcgacacac tgccgttagt gctgaccagc acacgttccg
      661 tcagttgcag gttaaggctg atatcacaga catcgttgcg cagaatatcc acctcaaagg
      721 tgaatagctt ttcccgtaac gccgggttat tgatggcatc gggctggtta tcccgcagcc
      781 acagtaaaac cggggccatc agcaaatttt ggtcgccgct gaaatcctct atcaccacgt
      841 tcagggtata gcggtactcc catgacatgg agctggcccc cgtggcaacc agcgaaccgt
      901 tatccacaaa cagatgcagt ttgtccgggt tattgcggac ataaggcact gctttattga
      961 gggcgtggcg cagggactgt ggtttgttca ctgtttcgct cctgacacgc aataatcatg
     1021 tccactttgt ctgcacagac cgcccaggcg gcctccgttt catccagcaa tgcgttcaga
     1081 tcaccgttag tgcgcggcgc agcctgctcc agccgacacg gcgtcactcg cggacaacca
     1141 ctgacggtaa gctgcacctc cggtaagtgc cggacgttcc cgcagccgga taatgtcagc
     1201 aggcaaagga gtatcagccc agcggtgtaa atcctcgttc tcacgtttca gttcctcgat
     1261 ccggcgttgt cgttgtctca gcagtgcact ggtctgttct gcttcggcgt agagccgcgc
     1321 ctgctcccgg ttgttggttt cagccagaat ggatagaccg atcagctggc tgtttttctt
     1381 cgtcagttcc tgcgctttac ttttcagcgc cgcgcgctgc gtttcgatgg tgtggctggc
     1441 gctgttaagc cgccacgact gccagcccag cgcaacgagt gccagtgcca ccactactgc
     1501 cagcgcacgc gtcatagtcc agcgccttta aggcaccagg ccatctcctg cgcacggcgg
     1561 ttatccagcc cctgattaaa cacacctttc acataaaccc agcgcggcaa ctgtcggcac
     1621 gcatccgccc agcgccgctg gttgagcatt ttcaccagcg tggaactgca ggcattgccc
     1681 gttcccacgt tgaaggcaaa cgacaccacc gcgtcataca ccttctgcgg cggctgttgc
     1741 ttcacacacc tttccagcgc ctgctccaca cgtataacgt tggaaatcag tccttctgct
     1801 gcctgtcgtt ccgtaatggt tttgccggga atgacgcccg acgtattacc aatgccatcg
     1861 gtccatacac ccgcgctgca ctgatacggc tgcagacgac agccttcgta atcggcaatc
     1921 agtttcagcc cctccacgga ggtgtgaagc tgctgaaaac ccggcagcgt ggcagcaata
     1981 gccagcacgg ccccgacaag gcagcgttta acgattgatg gattcataat cctcccgcga
     2041 gatctgtccg tcgcgcagaa gctggtaggc tttgtgtttg tagtaccagt tgatagccag
     2101 catcagcaca ccgatcatca ggccgcccag cgttgaggca tccttgatgg acaaatcgcc
     2161 cagccaggcc agcacgacgg cgatgcaata cgtgataaag gcgctgattc gctcaagcgt
     2221 cataattcag tcccatagct ggacggtctg cacggtggtg gttgtcggaa tgtccggcag
     2281 ctccacctgc agcccgtgag gtaaaaaggg gccgtattcg gcaagccccg gatttgcctt
     2341 cagtacctgc tccgtgacac cctgcgtgcg cccgtaatga cgccagcaaa gcgcgtccac
     2401 cgtgtcatac tgatgcgcac gcactttcat cagataagct ccactgtgca gtgcggcgca
     2461 tcctgcaccc ggctgatggc ccagcgggcg tcacgccaca aatcaccgct ggattccgcc
     2521 agttcctcgc ctcgcttcac accggatgcc gtggcgtcat agtcctggta tcgttcgttg
     2581 agcatggcgc gtgcccagca gtaaaccgcg ttgaaatagt gctgaatgcg ctcacttttg
     2641 ccgtccagct gttccgccgg aacctcagcc agcgacacat atcccagcat ctgctgacgt
     2701 ctgcgaaact catacagctc tgcgttgacc tccgaaattg ccgacagggc aacctgcttt
     2761 aaacgcggct gcgtcaccgt gccgtcagtg cgcatcacgc tgcgaaactc cgacaggtcc
     2821 acatcaggcc agaacggcgt atttctgatg atttccgcct gttccggtgc ctgttctggc
     2881 gcaacaaact tcatgctgct ttctcctgaa atagagggcg gtggacggga ttttgatgtg
     2941 gctatgcctt tcgccacccc gtgccgcccg tgcgcggggg cacgttctgt cagcggctgt
     3001 cattgcgcag tctgcgctcc agctgctgtt tgtctttttt cacgccacag cggggatcga
     3061 gctgtaacgc atggttgaga tggttaaggg cagaagccgg attgctttca ctcaggacag
     3121 cgccaatcgc tttatgcaga cgcgctcgtg actggtccgg catatccaga ccgtctgtca
     3181 gctccagcgt ctgcaacaac agatcggcat caaagccggt ggcggcaagc attgcgctct
     3241 gcgccgcgtc tgccatttcc tctgccagca cggtctgcac attgcggtta cccagcggca
     3301 tcacccagcc atgacgcagg gcatgacgcc cgatctccag cgccccggca taatctccgg
     3361 catcaatgcg ccacagcatc acgtacatca gcacgtcatc ctgctgtgcg cctccggcag
     3421 tcaggacacc ctctgcccag gcggcgtact tcggcagcag ctccacctta atttccgctt
     3481 ttttcaccgt ggactgaacg cccttgagac ggcggcggtc ttccgccagt tgcagcagca
     3541 tcaggtcata gcccgacgcg tggcgaacac tgccgccctc gcgggcggcc tgttcagcct
     3601 gaacgcgcag gcgatgctgc cgtgcgggac tcaggctcat ggtttatgct ccggcttctg
     3661 cggcggcgct gaagtcacca atctggatgt tttccaccag tgctgcgcag cggtagtcct
     3721 caaccacata ggcttcgtta actgattcaa agttttcaat ccggtcacgt ttcgggttgt
     3781 cgataaccga acggcggcgg gtgtcctcct gccagtaaat ggacaggtta tccagacggg
     3841 tgatcagcag cgcattcggc gggaagaacg gcgcacgcac ggcctgcagg ccccccatgc
     3901 gtttctgact gatgatcata tcggcagcca gtttttcact gttttcctgg tctttgttga
     3961 ccagcgggaa atacttgtca gacagcagct cacgaccgca aatcaccacc agatcgtcat
     4021 cgtcctggta gaccacgtcg ataagctcat tgaccgcatc catcaccacg gcgtccaggt
     4081 tggcatattc gccacctttc ccgactttca ccgcacccgg tgtggtttca ccgcccgtgg
     4141 tggtgctgcc catgacgtga tccggtgcat cctcacggat tttctgcagc cagcctttgt
     4201 tcacatcctg cagcagcggg ttttcgctac ggttagaggt tttcgcacgc ttcacgccgt
     4261 taaagccgat catgatgcgg tccagcgcct gacgtttcac gatggcgtta cggatacgca
     4321 cctggaaatc ctgaaacttc gcccacaggt ccagcttcgc gtaggtcagc accgtatcaa
     4381 agttggtctg ttcgcatttg tattccacat cgaccatcag cgtcggatcg acaggttcac
     4441 gctctttcgc ggtggtgtca gtggttccgg caatggtgct gccaaccccc agccccagca
     4501 gctgaccgga ctgctcagtc actggcgtga cgttaatcag cgtcaggaaa gcggcggact
     4561 gctggatctg gtcttccagc gtctgctgca cagacggctc tacagtgaac ttgctggaca
     4621 gttcttcaac tgccacaccg ttcagacgcg ccagttgctg caggtaagcg ttaaaagcaa
     4681 agcgggtatt cttcttcatc aggttttgtg ctccatcagc aattggtcag agtgtcagcg
     4741 ggggcgttac cgcctgttac acgctggcgg tagtcctggc ggctgtcttc atgactcagc
     4801 ttattcacca gttcgttaaa ggcggtttgc tgctcctgca gagcagtctc cagctcagac
     4861 aggcgttctt cctgctcaga cagggatttt tcggtgcgtg cgctcaggtt ctgctgctca
     4921 gtgacgacca gctccacggc cttatgcaca tcagcgaacc gggcgtcatc ggactgctct
     4981 tttttggtaa acaacgccgt gacgcgggca aacagggacg gtttgtcatc ctggacttct
     5041 tccagttcga tcaccgtttc ctctgcagcg gtaaaaagat tggcgggatt ctgcttgcgg
     5101 tttgccagcg ggttatgggc tgcactggcg ctgaatgtca gcatttccgt acccagactg
     5161 gcaggatcat cagtggcagc taggccaacc aggtaggctt tgcccgtatc agcgaacttc
     5221 gggctgactt ccatagaggt gaataatttc tggccttttt tcaccagctc caccagggac
     5281 tccgttggct caacgtcggc atacagcgcc atcttgcctg ccagcggacc ttccgtgatt
     5341 tcttcagcaa acagcgccgt cactttgccg tagcggttaa aggtgctgtc cggcagataa
     5401 gacttgatgt gctcaaggtt aatcagcgcg gtatacaccg ccgggttgta gctggctgcc
     5461 atctgttcca gccattcacg ctggatttcg cgtccgtcgg tagtggcacc ttccaccccg
     5521 atgcgaaaac gctttgcttt cactgtcatg agccgtgctc cgttagaaaa aacttactgg
     5581 agccttatgg ttgcggtgat gggggcagtg aaacaatgcg cggtatttgt accgacaacc
     5641 acacaaaccg caggcgggga aagccgtcat tcaaggctgt aggtttgtgc catgaacacc
     5701 acactgacac ccgcagatct cgatccccgt cggcaggcca tgctgctgta ctttcaggga
     5761 taccgcgtag cccgcattgc tgaaatgctg ggcgagaaag ttgcaaccgt tcacagctgg
     5821 aaaaaacgcg acaagtgggg tgactatggg ccgctggatc agatgcagct caccaccgcc
     5881 gcacgctact gccagctcat tatgaaggag cacaaagaag ggaaagattt caaagagatt
     5941 gacctgctgg cgcgccagtc tgagcgccac gcgcggatcg gcaagtttaa caatggcggc
     6001 aacgaagccg acttaaaccc taacgtcgcc aaccgcaaca aaggcccgcg ccgtcagccg
     6061 gaaaagaatg ttttcaccga tgaacaaatt gagaagctgg aagaaatttt ccattcctcc
     6121 atgttcaact accagcgcca ctggtgggaa gccggaaaaa ccaaccgcat ccgcaacctg
     6181 ctgaagtcac gccagatcgg cgcgaccttc tattttgccc gtgaagccct gattgacgcc
     6241 ctgctgaccg ggcgtaacca gattttcctt tccgccagca aggctcaggc ccacgtcttt
     6301 aagcagtaca tcatcgactt cgccaaagaa gtggaggtgg agctgaaagg cgatccgatg
     6361 gtgcttccta acggggccac gctgtacttc ctcggcacca atgcccgcac ggcccagagt
     6421 taccacggca acctgtatct ggatgaatat ttctggatac cgaaattcca ggagctgcgc
     6481 aaagtggctt ccggtatggc tattcacaaa aaatggcgac aaacctattt ttccacgcca
     6541 tccagcctga cacacagtgc ttatccgttc tggtccggtg cgctgttcaa ccgagggcgc
     6601 aacaaagccg ataaggtgga catcgacctg tcccacagca atctggcccc cggcctgctg
     6661 tgcgcagacg ggcagtaccg ccagatagtc actgtggaag atgcggtgcg cggcggctgt
     6721 aacctattcg acctcgacca gttgcgcatg gagtacagcc cggacgaata ccagaacctg
     6781 ctgatgtgtg agttcgtgga cgatctcgcg tccgtgttcc cgctcagcga gctgcaggcg
     6841 tgcatggtgg acagttggga agtctggacc gactttcatg cactggccct gcgcccgttt
     6901 ggctggcgcg aagtgtggat cggatatgac ccggcgaaag gtacgcagaa cggcgacagc
     6961 gccggatgcg tggtggtggc tccgccagcc gtgccgggcg gtaagttccg cattcttgag
     7021 cgtcaccagt ggcgcggaat ggacttccgc gcccaggctg acgccatcaa aaaactgacc
     7081 gaacagtaca acgtgaccta tatcggtatc gactcaaccg gcgttggtca cggggtttac
     7141 gagaacgtga aagcgttttt tcctgccgtc cgggagtttg tctacaaccc caacgttaaa
     7201 aacgccctgg tactcaaggc ctacgacatt atcagccacc gtcgtctgga gtttgacgcc
     7261 ggacacaccg acatagcgca gtcctttatg gcaatccgtc gcgccaccac cgccagtggc
     7321 aaccgcccga cctatgaagc cagccgcagc gaagaagcca gccacgccga tctggcctgg
     7381 gcaacgatgc acgcactgtt taacgaaccg ctgcagggcg aatccgccaa taccagcaat
     7441 attgtggaga ttttttgatg ggaaagagta agaagaaccg cgctgcggcg acgaaacaga
     7501 tccagcttaa aagtcaaact acagccgaag cattcagctt cggcgatccc gttcctgttc
     7561 tggaccgccg agaactgctg gattatgtgg aatgcgtaca gatggaccgc tggtatgagc
     7621 cgcccgtcag ctttgacgga ctggcgcgca ccttccgcgc tgccgtgcat catagttccc
     7681 cgattgcagt aaagtgcaac attctgacca gcacctacat ccctcacccg ctgctcagcc
     7741 agcaggcttt ttcgcgtttt gtgcaggact atctggtatt tggtaacgcg tacctggaga
     7801 aacgcacgaa ccgcttcggt gaagttatcg cccttgaacc tgccctggca aaatacaccc
     7861 gacgcgggtt agacctggat acctactggt ttgtgcaata cggtatgaca acccagccgt
     7921 atcagttcac gaaaggcagc atctttcatc tgatggaacc ggacatcaac caggagatct
     7981 acggcctgcc cggttatctt tctgccatcc catccgcttt gctcaacgag tccgccacgc
     8041 tgttccgccg caagtattac atcaacggca gccatgcagg cttcatcatg tatatgaccg
     8101 atgccgcgca gaatcaggag gatgtgaaca atctccgcaa tgcgatgaaa agcgccaaag
     8161 gccctggcaa cttccgcaac ctgtttatgt actcgcctaa cggcaaaaag gacgggattc
     8221 agatcatccc gttgtcagaa gtcgcggcga aggatgagtt tctgaatatc aaaaatgtca
     8281 gccgcgatga catgatggca gcgcatcgtg tgccgccgca aatgatgggg attatgccga
     8341 ataatgttgg ggggtttggg gatgtggaga aggcaagtaa tgtatttgtt cgtaacgaac
     8401 ttat
//
LOCUS       DAJOTB010000188         8321 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJOTB010000188 DAJOTB010000000
VERSION     DAJOTB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.188"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            347..1330
                     /locus_tag="OHN28_004313"
     CDS             347..1330
                     /locus_tag="OHN28_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HCQ2299594.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVIISGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPSNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1402..2550
                     /locus_tag="OHN28_004314"
     CDS             1402..2550
                     /locus_tag="OHN28_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HCQ2299595.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEQSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2574..4250
                     /locus_tag="OHN28_004315"
     CDS             2574..4250
                     /locus_tag="OHN28_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HCQ2299596.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4260..5000
                     /gene="kdsB"
                     /locus_tag="OHN28_004316"
     CDS             4260..5000
                     /gene="kdsB"
                     /locus_tag="OHN28_004316"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCQ2299597.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDTALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4997..7024
                     /locus_tag="OHN28_004317"
     CDS             4997..7024
                     /locus_tag="OHN28_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HCQ2299598.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVLQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSDLFIVLQWLQLQRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLINHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDISAELTAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTVDEHHCPRRERRLTIADLIYQALIVYPTYI
                     HPTQLQPITVEEAAEYLIQTPRKSMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            7059..8255
                     /locus_tag="OHN28_004318"
     CDS             7059..8255
                     /locus_tag="OHN28_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCQ2299599.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVA
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYDLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDIKLFKKFRGYLLVKTQVNAVYYGGN
                     TKKTPT"
BASE COUNT         2162 a         1981 c         2144 g         2034 t
ORIGIN      
        1 caaggaaata atcaattgat aaattgtttt tgagcttaat agtcaagtgg gaaacatttt
       61 aataaatgat aaaaatccta aattccttgt tcataatgta ggggtgtgtt aaataggtgg
      121 gcaaaatttg gttccccttc tcgcctgtaa tattgcagca tcttcgcgcc tccatgagac
      181 attgcgactt aataagaagg tgataagtcc tgcatataag catggactga ccatggtttt
      241 atattcatta aaaattttgt tacaacccat tgatttagca taaataaatt atagtgggtt
      301 cgggtttgtt gtgactgtgg cattatttac gtgcaaagga gctgatatgt ctgaaagaca
      361 tttacctgat gaccagagca gtactatcga tccatatcta attacctctg ttcgccagac
      421 tctggcagaa caaggcgcag cattacaaaa cttgtctaaa caactggatt ccgggcagta
      481 ccagcgtgtc cttaatttga taatgaactg taaagggcac gttattattt cgggaatggg
      541 taaatcaggg catgtcggtc gtaaaatgtc agcgacgctg gcctctacgg gtacgcctag
      601 tttctttatt catcctgcag aagctttcca tggcgatctg ggcatgatta cgccttacga
      661 tcttctgatc cttatttctg ccagcggtga aacggatgaa atcctcaagc tagttccttc
      721 actgaaaaat ttcggcaacc gaattatcgc cattaccaat aatggaaatt ccacgctggc
      781 gaaaaatgct gatgccgtgc tggaactcca catggcgaat gaaacctgcc cgagtaatct
      841 tgcaccaaca acgtctacca cgctgacgat ggcgatcggc gatgcgctgg cgattgccat
      901 gatccaccaa cgcaaattta tgccgaatga ttttgcgcgc tatcacccgg gcggttcatt
      961 aggtcgtcgc ctgctgaccc gcgttgctga tgtcatgcag catgatgttc ctgcggtaca
     1021 gctggatgcg tcatttaaaa ccgtgattca acgtatcacc agcggatgcc agggaatggt
     1081 gatggtagaa gacgcagaag gtgggctagc gggcattatc accgacggtg acctgcgtcg
     1141 ctttatggaa aaagaggatt ctctgacatc cgccacggct gcgcagatga tgacacgtga
     1201 accgctgacg ctaccggaag acaccatgat cattgaagcg gaagaaaaaa tgcaaaagca
     1261 ccgcgtctca acgttattgg tgaccaacaa ggcaaataaa gtcactggcc ttgtgcgcat
     1321 tttcgactaa ttaagcaacg gggttcgaga ggttagtctc tcattgagtt tgcgccccac
     1381 tgaaactttg ataatcgtta catgttgata aaagtgaagt ctgccgtatc ctggatgcgt
     1441 gctcgtctgt ctgccatctc actggcagat atccaaaaac acctggcgaa aatcatcatt
     1501 ctggcaccga tggcggtgct gctgatctat ctggctatct tcagccagcc tcgctatatg
     1561 agcgagtcga aagtcgccat taaacgctcg gatgatttaa acagcggcag cctgaatttt
     1621 ggtctgcttc tgggtgcctc taaccccagt tccgcagaag atgcgttgta tctgaaagag
     1681 tacatcaact cgccggatat gctggcggcg ctggataagc aactaaattt tcgtgaagcg
     1741 tttagccaca gcgggctcga ttttcttaat catcttagca aggatgaaac cgcagaaggc
     1801 ttcctgaagt actacaagga ccgtatcaac gtctcgtatg acgataaaac cggattactg
     1861 aatattcaga cgcagggctt tagcccggag tttgcgctta agtttaacca aaccgtgctg
     1921 aaagagtcag agcgctttat caatgagatg tcacatcgca tcgcgcgtga ccagcttgcc
     1981 tttgcagaaa cggagatgga aaaggcacgc cagcgtctgg acgccagcaa agcggaattg
     2041 ctctcttatc aggacaacaa caacgttctg gatccacagg cacaggcaca ggcggcgagc
     2101 acgttagtga atacgctgat gggccagaag atccagatgg aagcggacct gcggaacttg
     2161 ctgacgtatc tgcgtgagga cgccccgcaa gtagtgagtg cgcgtaatgc gattcagtca
     2221 ttgcaggcac aaattgacga agaacaaagt aaaatcactg cgccacaggg tgacaaacta
     2281 aaccgtatgg cagtggattt tgaagaaatc aaatcaaaag tagagttcaa caccgagctg
     2341 tacaaactga ccctgacctc cattgaaaag acccgtgtag aagcggctcg taagctcaag
     2401 gtgctgtcag tgatcagttc gccacagttg ccgcaggaat cgtcttttcc aaatatccct
     2461 tatttgatcg cctgttggtt actggtgtgc tgcctgctgt tcggcaccct gaaactgttg
     2521 ctggctgtta ttgaagatca ccgagactaa cgctgtcgct gaatgagttt gtgatgaaat
     2581 tatttaaatc cattttactg attgccgcct gtcacgcggc gcaggccagc gcggccattg
     2641 atattaacgc tgacccaaac ctgacaggag ccgcgccact taccggtatt ctgaacgggc
     2701 aacagtcgga tacgcaaaac atgagcggct tcgacaatac cccgccgccc gcaccgccgg
     2761 tggtaatgag ccgtatgttt ggtgctcaac ttttcaacgg caccagcgcg gatagcggtg
     2821 cgacggtagg attcaaccct gactatattc tgaatccggg tgatagcatt caggtccgct
     2881 tatggggtgc gttcaccttt gatggtgcgt tacaggttga tcccaaaggt aatattttcc
     2941 tgccgaacgt tggtccggtg aaagttgctg gcgtcagtaa tagtcagcta aatgccctgg
     3001 tcacatccaa agtgaaggaa gtataccagt ccaacgtcaa cgtctacgcc tccttattac
     3061 aggcgcagcc agtaaaagtg tacgtgaccg gatttgtgcg taatcctggt ctgtatggcg
     3121 gtgtgacgtc tgattcgtta ctcaattatc tgatcaaggc tggcggcgtt gatccagagc
     3181 gcggaagtta cgttgatatt gtggtcaagc gcggtaaccg cgtgcgctcc aacgtcaacc
     3241 tgtacgactt cctgctgaac ggcaaactgg ggctttcgca gttcgccgat ggtgacacca
     3301 tcatcgtcgg gccgcgtcag catactttca gcgttcaggg cgatgtcttt aacagctacg
     3361 actttgagtt ccgcgaaagc agcattcccg taacggaagc gttgagctgg gcgcgcccta
     3421 agcctggcgc gactcacatt acgattatgc gtaaacaggg gctgcaaaaa cgcagcgaat
     3481 actatccgat cagttctgcg ccaggccgta tgttgcaaaa tggcgatacc ttaatcgtga
     3541 gcactgaccg ctatgccggc accattcagg tgcgggttga aggcgcacac tccggtgaac
     3601 atgccatggt actgccttat ggttccacta tgcgtgcggt tctggaaaaa gtccgcccga
     3661 acagcatgtc gcagatgaac gcagttcagc tttatcgccc atcagtggct cagcgtcaga
     3721 aagagatgct gaatctctcg ctgcaaaagc tggaggaagc ctcactttct gcccagtcat
     3781 ccaccaaaga agaagccagc ctgcgaatgc aggaagcaca actgatcagc cgctttgtgg
     3841 cgaaagcacg caccgtggtt ccgaaaggtg aagtgatcct caacgaatcc aatattgatt
     3901 ctgttctgct tgaagatggc gacgtcatca atattccgga gaaaacatcg ctggttatgg
     3961 ttcatggcga agtgctgttc ccgaacgcgg tgagctggca gaagggtatg accaccgagg
     4021 attacatcga gaaatgtggt ggcctgacgc aaaaatcggg taacgccaga attatcgtca
     4081 ttcgtcagaa cggtgcagcc gtcaacgcag aagatgtgga ttcactcaaa ccgggcgatg
     4141 agattatggt tctgccgaaa tatgaatcga aaaacattga agttacccgt ggtatttcca
     4201 ccatcctcta tcagctggcg gtgggtgcaa aagtgattct gtctttgtaa ggggttgaga
     4261 tgagcaaagc agttattgtc attccggctc gctatggctc ctcgcgcctg ccgggtaagc
     4321 cactgctcga tattgttggt aaaccgatga tccagcatgt ttacgagcgt gcgttacagg
     4381 tggcgggcgt tgcggaagtt tgggtggcaa cagacgaccc gcgtgttgaa aaggccgtgc
     4441 aggcatttgg cgggaaagcc atcatgacgc gcaacgatca tgaatccggc accgatcggc
     4501 tggtcgaggt gatgcataaa gtcgaggcag atatctacat taacctgcag ggcgacgaac
     4561 caatgattcg cccgcgggat gtagaaacgc tgttacaagg aatgcgtgat gacaccgcgt
     4621 tgccagtggc aacgctatgc cacgcgattt ctgccgaaga agcgaccgag ccaagcacgg
     4681 taaaagtggt tgtgaatacc cgccaggatg cgctttattt cagccgttcg ccgattccgt
     4741 atccgcgaaa tgctgaaaaa gcgcgctacc tgaaacacgt cggtatttac gcttatcgtc
     4801 gcgatgtgct gcaaaactac agccagttac cggagtccat gccagagcag gcagaatcac
     4861 tggagcagct gcggttgatg agcgcaggga tcaacatccg cacatttgag gttgccgcaa
     4921 ccggtccggg cgtcgacacc ccagcatgcc tggaaaaagt gcgcgccctg atggcacagg
     4981 aactggctga aaacgcatga ttggcattta ctcacctggc atctggcgta ttccgcatct
     5041 ggagaaattt ctggcgcaac cgtgccagaa actttctctg ctgcgtcctg ttctgcaaga
     5101 agttgatgct atcgccgtgt ggggacatcg tcccagcgcg gcgaaaccag tcgccatcgc
     5161 caaagcagcg ggaaaacccg tcattcgtct ggaagatgga tttgtgcgtt cgctggatct
     5221 tggcgtcaat ggcgagccgc cgctttctct ggtggtggat gattgtggca tttactacga
     5281 tgccagcaag ccttcagcac tggagaaact ggtacaggat aaagccggaa atacagctct
     5341 gataagccag gccagagaag cgatgcacac catcgtgacc ggggatttgt cgaaatataa
     5401 cctggcacct gcgtttgtgg ctgatgagtc agaacgttca gacatcgttc tggttgtcga
     5461 tcagacattt aatgatatgt cagtgacgta tggcaatgct ggcccgcatg agtttgctgc
     5521 catgctggaa gccgcaatgg cggaaaatcc tcaagccgaa atttgggtga aggtgcaccc
     5581 agatgtactg gaaggaaaga aaacaggtta tttcgccgat ctgcgcgcca cgcaacgagt
     5641 acgtttaatt gccgagaatg tcagcccgca gtcgctgttg cgacacgttt cccgggttta
     5701 cgtcgtgacc tcccagtacg gctttgaagc cttgctggca ggaaaaccag taacatgttt
     5761 cggccagccc tggtatgcag gttggggctt aaccgacgat cgtcatccgc agtccgcttt
     5821 gttatctgcc cgacgcggtt ctgccacgct ggaggaactt tttgccgctg catacctgcg
     5881 ttactgtcgc tatatcgatc cgcaaacggg agaagtaagc gatctattta tcgtgctgca
     5941 atggctgcaa ttacagcgca accatcagca gcagcgtaat ggttatttat gggcgccagg
     6001 cttaacgctg tggaagtcag cgatcctgaa acctttcttg caaacggcaa caaaccggct
     6061 gagtttttca cgtcgctgta ctgcggcgag cgcctgcgtg gtatggggtg taaagggaga
     6121 acagcaatgg cgagccgaag cgcagcgaaa atcactgccg ttatggcgaa tggaagatgg
     6181 ttttctgcgt tcatccggac ttggctctga ccttttgccg ccgctatcgt tggtactgga
     6241 taaacgcggg atctactatg acgccacacg ccccagcgac ctggaagtgc tgattaatca
     6301 cagccagtta acgctggcgc agcagatgcg agctgaaaaa ttacgccagc ggctagttga
     6361 aagtaaactg agcaagtaca acctgggagc cgatttctct ctgcctgccg aggccaaaga
     6421 taaaaaaatt atcctggtgc cgggtcaggt agaagacgat gcctcgatta aaaccggcac
     6481 cgtctcgatt aagagcaacc ttgagttatt acgcacagta cgcgagcgta atccgcacgc
     6541 ttacattatt tataaaccgc acccggatgt actggtgggg aatcgcaagg gcgatatttc
     6601 ggcagaattg actgctgaac ttgctgatta tcaggcactg gacgcagata ttattcaatg
     6661 cattcagcgc gcagatgaag tgcacaccat gacgtcgctg tcgggatttg aagcgttatt
     6721 acatggtaag cacgtacatt gttacggcct gcccttctat gccggttggg gtttaaccgt
     6781 tgatgaacat cactgcccgc gccgcgagcg cagattaacg atagcagact tgatctatca
     6841 ggcgctgatt gtttatccaa cctatatcca cccaacacag ctacaaccta ttacggttga
     6901 agaagcggcg gaatatttga tccagacgcc gcgcaagtcg atgtttatta cccgaaaaaa
     6961 agcggggcga gtaatacgct attaccgcaa attaattatg ttctgcaagg tcagatttgg
     7021 ctaaacaatt tcatagttga ctattacgca aatcatttat gcaaggtaat gcactaaccg
     7081 ttttattatc cggtaaaaaa tatctgctat tgcaggggcc gatgggaccc tttttcagtg
     7141 atgttgccga gtggctagag tcattaggcc gtaacgctgt gaatgttgcc ttcaacggtg
     7201 gggatcgttt ttactgccgc catcgacaat acctggctta ctaccaaacg ccgaaagagt
     7261 ttcccggatg gttacgggat ctccaccgac aatatgactt tgataccatc ctctgctttg
     7321 gtgactgccg cccattgcac aaagaagcaa aacgttgggc aaagtcgaaa gggatccgct
     7381 ttctggcatt tgaagaagga tatttacgtc cgcagtttat tactgttgaa gaaggcggag
     7441 tgaacgcata ttcatcgcta ccgcgcgatc cggattttta tcgtaagtta ccagatatgc
     7501 ctgcgccgca cgttgagaac ttaaaacctt caacgatgaa acgtataggt catgcgatgt
     7561 ggtattacct gatgggttgg cattaccgtc atgagttccc tcgctaccgc caccacaaat
     7621 cgttttcccc ctggtatgag gctcgttgct gggttcgtgc atactggcgc aagcaacttt
     7681 acaaggtaac acagcgtaag gtattgccga ggttaatgaa tgagctggat cagcgttatt
     7741 atcttgccgt tttgcaggtg tataacgata gccagattcg taaccacagc aattataacg
     7801 atgtgcgtga ctatattaat gaagtcatgt actcattttc acgtaaagca ccgaaagaaa
     7861 gttatttggt gatcaagcac catccaatgg atcgtggtca cagactctat cgaccattaa
     7921 ttaagcggtt gagtaaggaa tatgacttag gtgagcgagt catatacgtg cacgatctcc
     7981 cgatgccgga attattacgc cacgcaaaag cggtggtgac aattaacagt acggcgggga
     8041 tctctgcgct gattcataac aaaccactca aagtgatggg caatgccctg tacgacatca
     8101 aaggattgac gtatcaaggg catttgcacc agttctggca ggccgatttt aaaccagata
     8161 taaaactgtt taagaagttt cgcgggtatt tattggttaa gacgcaggtt aatgcggttt
     8221 attatggggg gaacacaaaa aaaacgccaa cataatacat attaaatttc aagtcaatat
     8281 tttttgaatt ttaagtagct aaaaaatcat ttgcatcctc t
//
LOCUS       DAJOTB010000189         8307 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJOTB010000189 DAJOTB010000000
VERSION     DAJOTB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.189"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            125..556
                     /gene="osmC"
                     /locus_tag="OHN28_004319"
     CDS             125..556
                     /gene="osmC"
                     /locus_tag="OHN28_004319"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCQ2299600.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(746..3145)
                     /gene="dosP"
                     /locus_tag="OHN28_004320"
                     /gene_synonym="pdeO"
     CDS             complement(746..3145)
                     /gene="dosP"
                     /locus_tag="OHN28_004320"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCQ2299601.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3170..3333)
                     /locus_tag="OHN28_004321"
                     /pseudo
     CDS             complement(3170..3333)
                     /locus_tag="OHN28_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3711..5030)
                     /locus_tag="OHN28_004322"
     CDS             complement(3711..5030)
                     /locus_tag="OHN28_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCQ2299602.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5161..6696)
                     /gene="gadC"
                     /locus_tag="OHN28_004323"
     CDS             complement(5161..6696)
                     /gene="gadC"
                     /locus_tag="OHN28_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCQ2299603.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(6852..8252)
                     /locus_tag="OHN28_004324"
     CDS             complement(6852..8252)
                     /locus_tag="OHN28_004324"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2299604.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         2110 a         2049 c         2082 g         2066 t
ORIGIN      
        1 gtggaaattt aatttaagtg cagaaattat attttcgccg gattttattc ggattatcct
       61 gcttatcctc gtgctgtttc tcacgtagtc tataatttcc ttttaagccc acaggagagc
      121 aacaatgaca atccataaga aaggtcaggc acattgggaa ggcgatatca aacgcgggaa
      181 gggaacagta tctactgaga gcggcgtgct gaaccaacag ccgtatggat ttaacacgcg
      241 ttttgaaggc gaaaaaggaa ccaaccctga agaactgatt ggcgcagcgc atgccgcatg
      301 tttctcaatg gcgctttcat taatgctggg ggaagcggga ttcacgccaa catcgattga
      361 taccaccgcc gatgtctcgc tggataaagt ggatgccggt tttgcgatta ctaaaatcgc
      421 actgaagagc gaagttgcgg tgccgggtat tgatgcctcc acctttgacg gcattatcca
      481 gaaagcaaaa gcaggatgcc cggtttctca ggtactgaaa gcggaaatta cgctggatta
      541 ccagttgaaa tcgtaaagcg ttgccggatg acgcgtcagg cgcgtgaatg cctgatgcgt
      601 cagcacaatc acatcaggct gagaagggat tgttctgaca tggctatgcg ttgtcgttgt
      661 ccaactaaac gtagcggaaa tcacagcatt taagtgacag tgtcacgtta aataaaaacc
      721 cgcgagtgcg ggcgagagga atttatcaga ttttcagcgg taacacgctg ctcatccagc
      781 ctggaatttc ttcggcgggc agggggcggg aaaagaaata tccctgaata acgcgacagt
      841 ggatcttgcg tagcatctca aattgctctt tggtttcgac gccttccgcc acgacggtta
      901 aattgaggct ttgcccaatg ctggtaatgg cttcgagtaa ggcaaggatg cgtttttcag
      961 tcagacaacg atcgacaaaa cttttgtcaa ttttgatttc cgttaccgga agactgacta
     1021 agcgggataa tccggaaaag cccgtaccaa aatcatctac cgataaaccg atgcccatat
     1081 cgcgcaggat ctgaatgcgc ttaaagattt cggtatcgtg ttccatcatc atgctttccg
     1141 tgatttccac cgtcagctgg tggccgtcaa taccccaggc gtgcattgca tcagatacct
     1201 gattaggcag ctgattactg cgaaagtgca gcgcagacaa gttaacggat aacgccggga
     1261 tatgaatatt ctggctacgc cattctgcta actgacggca agcttccgcg atgacccagc
     1321 gcccaatatt ttcgatttca ccaatctctt ctgcgagagg aataaaccgt gaagggggca
     1381 catgaccatg caggggatcg taccagcgag caagggcttc gatgccgtac agttcacccg
     1441 tttctgcgaa gatttgcggc tgataaacca gtttaagttg gttattgcta atcgcttctt
     1501 ttagcgctgc gcctaaaacc aaacgctctt ttaccatttc gttcatcgcc gggctgaaga
     1561 actgccagcc gttaccgcca tttttgcgaa taaaatccat tgcattgtga gcagtggaga
     1621 gcaggtaatc gcggttttta cccacgtcgt agctgatgcc aatgctcaag gtgagcggga
     1681 atggtttatc gtcaatcatt atcggcttgc tgaccacatt ccgtagctca tcggcgatct
     1741 gggtaatgtt actgacatcg ttttccaggc tcacgaggac aaaagacgca ccttcaatac
     1801 gacagagata ctgatccggt ttgagttttt cacgaaagcg attgaccact tccagcaatg
     1861 cctgatcggc ccacgcatag ccaaggctat caatcacatc ctgaatatgg tcaacaccga
     1921 tgagatacac caccggagag acggctttgt cgaccaggtc atcgaggtaa ttgtgcaggt
     1981 tattgcgatt tggcagaccg gtcatcggat caaattggat gagttgttca atatgctgac
     2041 ggcttttttc ctgttccagc gccagcgcgg ccatatgctg gctgatatct gccacgcgtt
     2101 cgataaaggc gctggtttct gctcctgacg aggttttaat ttgcaggatc cccgcaggcg
     2161 cgccatcgcg ctgacgaatg gtcgctgacc agctttgcgc attttgaact tctgcaccgt
     2221 gcgaagatga cgcccagtgt atcggcatcc cgttgcgcag cgcgaacaga gaaacatgcg
     2281 attcgttgag aacagattcg atattacgac aaatgatttc ccccatttca tgaaatggtg
     2341 ggctgctgca catggcggcg agaatattgc cttcaagctg gcggatttgc ctttcttcgg
     2401 tgatatccga gaatgtcatt accaggttct gcagatgcgc gagcacgtca taaaccgggc
     2461 tgatagaggc tttaatccag attttttcac cggtgcgcgt caacagcaga aattcgtcct
     2521 gatcgcgggc ggttttccat agcaactgct gtaaacgaat acggttatca gcagggaatt
     2581 ctggaatgtt caggagtgta tcgggctgca taccgctggc ttcgttaatg cagtaaccaa
     2641 acatttcggt aaatgcgcga ttgcactgca caatatggcg ttccggatcg aggacaatca
     2701 ccggtcggtc gagatggtca acggcaataa tcaattgtcg ggtctgttct ttttgcgcca
     2761 tttctacgct ggcatcccgt accagcgcca ggtaataaac tttcccctcg gcgctcactt
     2821 tcgatagcgc aaaacgggtc cagattttac tgccgtcttt tttctccagc tgcagctccc
     2881 gactcatccc ctcgacgcgc gctttaccac cttcacggtt gtgacgaatg tattgaggat
     2941 gcgcaggacg caaatcccgc ggaatcagca tatcaatgtt attgccaatg acttcttcac
     3001 gtttgtaccc ccagagcttc tctgcggcgg ggttgaaaaa catcacttca tcattttcgt
     3061 taattaacac cgcgcccatc atattttgct caagggcggg gaaaaaaatg ccatcggcgg
     3121 cattatccgc atcggttagc ttcatgatta cctctgcatc ctggcgcatc taaagactgg
     3181 ctttacagag ttcaacacgg tttctacctc gtcttttgcg atatacagag cttcatcggc
     3241 tatttgaatg aggcgctcat agtcaggatg accattaaac atggcggcac cgatggaaag
     3301 tgagagggca atatcctcgc cgtttgcggc tttaaaataa tgtcgacctt tatggttaaa
     3361 ccgcaggcca aaatcaacca aacacaacgt gcgggattgg ctaactgcac catattctcc
     3421 tgaaatatga agatatactg aaaagaaata agcgacttag gacagtttca atctacgcta
     3481 ctgttcttca gaagagtata gcccatcgta attatttttc ggtgacagcg aatatcgtat
     3541 gttttttcat attcatacat ttttattagg gggattatgg ctgtttaact aagtgtggtt
     3601 aattgcgtgg ttaatttgac gtatttaagc atgattgcta gtgggatagt tcaagagggt
     3661 aacaagccag tgggtaaagc accggcttgt tacaaagtaa gaatgggagt ttaactgccc
     3721 caacgacttt gcagatagct gaccgcttgt tgagtctgcg gtttattcag atagtcctca
     3781 cggaacaaga ttgtgccgct aatttccggc acagcatcgt taagatcgag ctgctttttc
     3841 agttccggta cgccgccgtt aatcatccag tctggctcta tctttgacgg ttctcctact
     3901 ttatagaagg cgataccgat atacaggcgg gtcctggtcg gtttaacgac atccgcccac
     3961 cattttgcca acacgtcata acgcgcggca ctccgtgaga atggccagta aatttgggga
     4021 gcaatgtaat ccagcaaccc ttgttccacc catcgacggg tatcagcgta ggattcgtca
     4081 taggctgccg cgcctcgggt atcggaaccg agcggatcgt gtgatcggtt acgccacacg
     4141 cctgccgggc taacgccaaa ttcgacttcc ggcttaatgc ttttaatggt gtgcgaaacc
     4201 ttcgcaatta actgctgggt attgttgcgc cgccagtctg cttttgacgc aaatgcgcct
     4261 ccgtatttgc ggtacgtttc gttatcattt agccgtgaac ctggtgactc agtatagaaa
     4321 tagtcatcaa actgcacgcc atctaccgga tagcgggaaa ccacttccgc aactattgat
     4381 gtgatccagt cctgaacctc agggatgccc gggtcgagga caaagcgatc gccggacgtt
     4441 ctgatccagt cgcggtgttg cacatagacg ctggccggtt gttgagacag ggtgctattc
     4501 agttccctga tagtaccggg cttcgtatta accgatacgc gataggggtt aaaccaggcg
     4561 tgtactttca tcccacgctt gtgggcttcg tcgagcataa attgcagcgg atcgtaaccc
     4621 ggattttcac caatcttacc ggtcataaga tcggaccacg gcaaaatttt cgatggccac
     4681 agggcggtac catccggctt gacctgaaaa aagaccgtgt ttatgccgag acgttgcaga
     4741 tgatccagtt tgtcgatcat cgcctgttgt tgtacacgtg cccggctggt ggggttacta
     4801 ttgttaaccg aggaaaccgg tggccagtcg aggcgagaca ccgtggccag ccagatgcca
     4861 cgcatcggtt gtgacgattg ttgcgtcgtg gctggtggct ttgagcccgc tggtggtgtc
     4921 accatggact ctggaggcgt gcttttacaa ctacacagta aaagtgccag tgcaaccagt
     4981 atcgctggtc ttctaatcgc taatttctcg tttcgggagc agatatccat atgtgctggt
     5041 ttccggtaaa cagatgtgcg ctcattctcg tacttatccc ggttaagtca atacgacaac
     5101 aagcacgaaa aagggagcga tgaattatcg ctcccttgtc ttataagcat tcagacatgg
     5161 ttagtgtttc ttgtcattca tcacaatata gtgtggtgaa cgtgcacgcg ggtgcaggaa
     5221 gaagtgacct tttggtgcgt tctgactgtt gattggctcc agagtgacgc cggtattcgc
     5281 tttgctttta cgatcatgaa cagcatagag aataaagggc agggcaagta ccaccaggaa
     5341 actaacgacc agtaattcaa catacatatc ggtagagtca ccctggatgt tatccggcgg
     5401 caggaaggaa acaataaacg ccataattga agtcagcaga ccgacaatag ccacgaccag
     5461 tttcacccct ttaccaccag ggatattaaa tgtgcgtttt aagtcaggat gtttaagcac
     5521 caacacaatg tagccaataa acagcatgaa ataagcacac agataaatca ccaccgtcag
     5581 cgccagtgcg atcaggaagg acatgttgtt accgccaccg gtattggtga ggatgatcaa
     5641 cgcgatagaa gtaatcacca actgcgaaat gaccagcgtt accggtacgc catttttgtt
     5701 cattttagcg aatgccgctg gcagcaggtt tttctgcgct gtgacataca tcccgcgaga
     5761 aggaccaaca atccaggaag cgatttccgc cagaacaccc agcaacagca gtgcggagat
     5821 cacgcgaacc gtccactcaa tttccggtgc cacatgtgac atcagaacgg taaaggtttg
     5881 cattacccct gcggagaggt tgatttcatt acccggaatg accatcgcaa tagacaaacc
     5941 gccaacagag cttaggcaga ttgccgccac catcagcagt aacatagcca gtggatagtc
     6001 gcgcccgggg ttgctcattt cattgacgtg ggttgcggaa gcttcaacgc ccatataact
     6061 caaaatgaag gcaacaaata caaccagggt gcccacttta gagaagtcag ggaagaaggt
     6121 cttcgcatcc atttcgatag caacgggggc accggagtgc agataaatag ccgctaatgc
     6181 gatcaaaata aatgcaggta acaggatacc ggcgaagaag ccaactttag caattcgcgc
     6241 cgtgtatttc gtgccaccaa actgcgttaa tgccagcgcc caaagaatga tgagtgctgc
     6301 aatagttttg gtaatggggt cttcattcag cgctggccat ttcaggatgt aggagagtgc
     6361 ccctaacacg aaatagagca tcggaataaa accaatggcg atttgcagat agccaaatga
     6421 gatcgctgca aatccccatc tcggccccag agtatttgat acccaggcga agacgccacc
     6481 ttcttcccag ccgtcgacgg tagccatttc cgcagcacaa agtcccacgg gaataaacca
     6541 taaaatcccg cctaatagca ggaagaagac taatgaaaag cccgatgttg cgaaggtagg
     6601 gtattcataa acagccatta ccatcgatgc cgttatggca aaaaatccga gtaatgtgag
     6661 ctgcttagct ttacctgtct gtactgatgt agccatatta tccccctaaa acggtattcc
     6721 tgtcggaacc gcacctgttt tgttctgaag cgtattcaga acaatatttt ccgttgctaa
     6781 tgccagtgaa cagactttgg aaattgtccc gaaacggctt cgtttcggga caccgttacc
     6841 gttaaacgtt atcaggtgtg tttaaagctg ttctgctggg caataccctg cagtttcggg
     6901 tggtcgctga gatatttcag ggaggctttg tagtcttcca gcaacagttc agcaaagtcc
     6961 atttcgaagc cgcgacgaca cataatgcgc atcaccacga tgtcggtggc ttcaccgccg
     7021 agagtgaagg ccggaacctg ccagccgcgc agacgcagac gttcagagag gtcgtacagg
     7081 gtgtatcctg gatcttcacc atctttcagt ttgaagcaaa ccgccgggat gccttcgtcc
     7141 gggcgaccgg tacagatgaa ctcatacggc cccagtttgg cgatttcatc cgccagataa
     7201 gcggcaacct ggtaggaagc gttctgtact ttggtatagc cttcacgacc gaggcgcagg
     7261 aattcatagt actgtgcaat cacctgaccc gccgggcggg agaagttgat ggcgaaagta
     7321 ccaatctgac cgccgaggta gtcaacgttg aacaccagtt cctgcggcag cgcttcttca
     7381 tcacgccaga taacccagcc gcagcccagc ggagccagac cgaatttatg gcctgaagca
     7441 ctgatcgatt tcacacgcgg caggcggaag tcccagacga tatccggggc gacgaacggt
     7501 gccaggaagc caccgctggc ggcgtcgatg tgcatgtcga tgtcgatacc ggtgtcggcc
     7561 tggaatttat ccagcgcatc gtgcagcggc tgcgggaact catagttacc ggtgtaggtc
     7621 acgccgaaag tcggcaccac gccgatggtg ttttcgtcgc aggcttcaat catgcgtttc
     7681 gggtccataa acaactgacc ggggcgcata gggatctcac gcaactccac atcccagtag
     7741 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ccaggtttgg tttatccgtg
     7801 ggtttgcctg cggcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
     7861 atacaggcct cggaagagcc aatggtgttg gtgccaacgg cctgaccatt tttcggtgca
     7921 ggcgcatgcc acagatcggc aaccatgttt acgcaacgca ggtcgattgc tgcagattgc
     7981 ggatattctt ctttgtcgat ccagttttta ttgatcgaca aatccatcaa tttatggacg
     8041 ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct gacgagcgtt gccatcaaga
     8101 tataattcat cattgataat ctgaaatgca acatcatcgc gcatttcgtg cagcggaaat
     8161 cgttttgact ccgcgatagt agaaatggcc tttgcaccaa aacgtgaatc gagtagttct
     8221 gagcggaaat ccgttaacag cttctggtcc atttcgaact ccttaaattt atttgaaggc
     8281 aataaaaaag taggatttat ccgcaat
//
LOCUS       DAJOTB010000190         8289 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJOTB010000190 DAJOTB010000000
VERSION     DAJOTB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.190"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1299
                     /gene="yjeM"
                     /locus_tag="OHN28_004325"
     CDS             <1..1299
                     /gene="yjeM"
                     /locus_tag="OHN28_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCQ2299605.1"
                     /translation="GGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVSTSAKVWVPFST
                     FLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARITAVGGIAVMCL
                     NLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIFAYGGIEAVGG
                     LVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGSVNLGNITYVL
                     MKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYSPLKAIIQGTP
                     KALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNKLTLMANVSMT
                     LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANVFTIIQPVVEA
                     GDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            1397..1711
                     /locus_tag="OHN28_004326"
     CDS             1397..1711
                     /locus_tag="OHN28_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCQ2299606.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(1742..5065)
                     /gene="mscM"
                     /locus_tag="OHN28_004327"
     CDS             complement(1742..5065)
                     /gene="mscM"
                     /locus_tag="OHN28_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCQ2299607.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(5087..6055)
                     /gene="psd"
                     /locus_tag="OHN28_004328"
     CDS             complement(5087..6055)
                     /gene="psd"
                     /locus_tag="OHN28_004328"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCQ2299608.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(6152..7204)
                     /gene="rsgA"
                     /locus_tag="OHN28_004329"
     CDS             complement(6152..7204)
                     /gene="rsgA"
                     /locus_tag="OHN28_004329"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCQ2299609.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7299..7844
                     /gene="orn"
                     /locus_tag="OHN28_004330"
     CDS             7299..7844
                     /gene="orn"
                     /locus_tag="OHN28_004330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCQ2299610.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            8055..8130
                     /locus_tag="OHN28_004331"
     tRNA            8055..8130
                     /locus_tag="OHN28_004331"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8088..8090,aa:Gly,seq:gcc)
     gene            8167..8242
                     /locus_tag="OHN28_004332"
     tRNA            8167..8242
                     /locus_tag="OHN28_004332"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8200..8202,aa:Gly,seq:gcc)
BASE COUNT         1874 a         2157 c         2236 g         2022 t
ORIGIN      
        1 ggcggtatct attcctggat gaataatagt gtcggaccac gttttgcctt cattggtacg
       61 tttatgtggt tttcctctta tatcatctgg atggtgagta cctccgcgaa agtttgggta
      121 ccgttctcaa cattcctcta cggtagcgac atgacccagc actggcatat tgccggactg
      181 gagcctacgc aggtggttgg tctgctggca gtggcatgga tgattctggt caccgtcgtt
      241 gcttcaaaag ggattaataa aattgcccgc attactgcgg tgggcggtat tgcagtaatg
      301 tgtctgaatt tagtattgct gttagtaagc attactattt tgttattaaa tggtgggcat
      361 ttcgcgcagg atattaattt ccttgcatca ccgaacccag gttatcagtc cggtctggca
      421 atgctatcgt ttgtggtatt tgccattttt gcctatggcg gaattgaagc ggttggtggt
      481 ctggttgata aaacggaaaa tccagaaaag aactttgcca aaggtattgt ttttgccgct
      541 attgttattt caatcggtta ttcgctggca atatttttat ggggcgtcag cacaaactgg
      601 cagcaggtat taagtaatgg ttccgttaac ctcggcaata ttacctatgt gctgatgaag
      661 agcctcgggg tgacgctggg taacgcactg catttgtcac ctgaagcgtc attgtcgctg
      721 ggcgtatggt ttgcgcgtat taccggactg tcgatgttcc tcgcttatac cggtgcgttc
      781 tttacgcttt gctattcacc gctgaaagcc atcatccagg ggacgccgaa agcattgtgg
      841 ccggaaccga tgacgcgcct gaatgcgatg gggatgccgt ctatcgccat gtggatgcag
      901 tgtgggttgg ttactgtctt catcctgctg gtttcgtttg gtggcggtac cgcatcggcg
      961 ttctttaaca agctgacgct gatggcgaac gtgtctatga cgcttcctta cctgttcctc
     1021 gcgctggctt tcccgttctt taaagcacgt caggatctcg acagaccgtt tgtgattttc
     1081 aaaacgcgta tgtcggcaat gattgcgaca gtggttgtcg tactggtggt gacatttgcg
     1141 aacgtcttca ccatcattca acctgtggtt gaagccggag actgggacag cacattgtgg
     1201 atgattggcg gccctgtctt cttctcgctg ttagcgatgg cgatttacca gaactattgc
     1261 agcagagtgg taaaaaatcc gcagtgggtg gctgagtaat caaatgccct atcgtcggcc
     1321 tgacgatagg gcgtaatgac agggaattag tgagatttcg tatgcgctat agcgaaatta
     1381 aactatgggt tacctcatga gtgcgcgcat gtctgtcttg tgctgcatct ggttcattgt
     1441 ggcgttcctc tggataacca ttacctccgc gctggacaaa gagtggatga ttgatggacg
     1501 cgggattaac aacgtctgtg atgttttgat gtatctcgaa gatgacgata ctcgcgatgt
     1561 cggtgtgata atgacgttac cgctcttttt cccttttctc tggttcgctt tgtggcgaaa
     1621 aaaacgcggc tggtttatgt acgccaccgc gctggctatt ttcggctact ggttgtggca
     1681 gttttttctg cgctatcagt tttgcttgtg agccggatgg gttcatccgg ccacaaagac
     1741 cttacaaact tcccgcctga cgacctttgc ccgcagaagt taatgttctc cccgtttgtt
     1801 taccattcag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
     1861 cgtggaagcc agccagaatc agctggtgaa tctcatggcg tagcggcata cggtgaccca
     1921 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgctgcaga tccaccagga
     1981 agacttccgg cgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
     2041 tttccgtcac ttcttcactg ttggcattgg caggggccgg aatcgtcaac accacgcgcg
     2101 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
     2161 cgataatttc tttgcggtcc cagtcgctga tggtggtggc gcgggtgtta attttcgtca
     2221 cactaccggt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
     2281 ggataatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
     2341 caccgagcgc ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc
     2401 cgaccagccc gccaattagc atcagcagat atttggtgat ggtggtgatg gcgtagcccg
     2461 tacccggcgt taaatccagg tgctgcagaa tcgccagttc cagcagcgcg ggcaggttgc
     2521 gcaccagctg cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa
     2581 tcggctccag gctttctacg ccctgtaccg tggaagtgac atcccacagc gaaatatttt
     2641 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
     2701 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
     2761 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
     2821 cgccacgcgc acgctgcgct aacatctctg cccggcgatg ctttgcccgg tcaaacgcca
     2881 gcctgcggcg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
     2941 accagatggc aaccgaggtt tcaagcctcg ctaacagcgc ctgcgccgtt gccagataac
     3001 ccaccgccga cgccagaatg gcaaccagag gtgcgccaat catcatgttc cacagcatat
     3061 ggttggtaat gttgtcgccg ctgccttctt tgttgagata cagcgggatc ccggcctttt
     3121 tcaggctgag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
     3181 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg
     3241 gcacaataag cccgatgctc atcaggtagt aacgcatccc acgggaaaca cgttcgcgcg
     3301 gccagccaaa atgagcgata aacaagccgt tcgggcgagc aaaggtggcg caaatcatca
     3361 ctacccacag cagcggcacg gtggctgtta cgccatcacc aatcgcgacc gccagcggat
     3421 aaggccacgc ctcgcgcaag ccgtaaccca gcgtcatcca cagcacaggc agcggtgacg
     3481 cgacgagaat cgaccagaaa agagtgcgta acgtcagcca gaagtgatcc tgagtcactt
     3541 tgccgacttt cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac
     3601 tgcaaccgac cagaatcaac gcgccaaaca gcggcaaaat cgtctcttta ctggtcagca
     3661 tcatcacact ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat
     3721 cctgggcgat ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca
     3781 gatagcggtg cgtcgcttcg ttcacctctt tcagtgcatc ctccagttgc ccgttggaga
     3841 ctttcagctt ggtcagctcc agcagtagcg tgtcgccacc ctgcaacaat gagttcagca
     3901 actcacgctg agtgcgtagc tgtgcttcca gaatacggtt ttgctcagcg gtcagcggct
     3961 gaccgtcggc ctgatggatt tgccgtagca gcggctgttt attaagcaga tcctcataac
     4021 gtaaccgttg cacacgtaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt
     4081 ccggtagccg tgccacctgc gcccgcaacg cttcgccgag caggttagat gatcccagcc
     4141 attgcgactg ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag
     4201 cctgacgctg ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agtgccgccg
     4261 atagttcgcg gttaatttta aactgggcga cgatatcttt tggcaaatcg gcgctacttt
     4321 ccgccagttg ctcggtactt tccagcgccc gttccgcttc cagttggcgt tgactattca
     4381 actggttgcg tagtgcttgc aaatatgcat ccagctgctg gctctctttt tcggccagct
     4441 ctgagcgcaa gcgcgctaat tcctggcggt tattggcaga tagctgcgcc agctccagtt
     4501 catcaacgag cgctttcaga cgtgcggagt cagactgcaa cgcgaaattt tgtgcctgat
     4561 tgagcggggt attgccggta agcgtcccca ggcggcgctc gatttcattc aactgacggc
     4621 gggcatcggt ttgctgttgc ggcagttgat tcagggaatc ggcaatctcg cgggcgcgct
     4681 cctgctcttg ctgggcctga cggcttttat ccagcaactg actgctgatc tggagaattt
     4741 cctgattcag cgcatcgtta gacattcccg gcgacacgct gcgtggctcg tcacgcatgt
     4801 tgtttaactg cgcgcgcaga gtggcggaaa gtttcgggta attatcaatg acttcttgat
     4861 attgcttgat gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgga
     4921 gcgcctctac gacttccggc tgcgcaggtt tcgccgcttt tgcctgctcc agttcctggg
     4981 tgatttgttt gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca
     5041 tcagaaaagt gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt
     5101 ttttatcgtc aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg
     5161 gggctggttc agcatctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa
     5221 ttttcgtgac agacaggctc tccagttgct caaccaggtc cactttacct ggtgcaaaca
     5281 ggttgataac ggtagagcct agtttaaagc gacccatttc ctggcctttc agcagtgcca
     5341 cagaaccgtc gttttccccg gcaggccagg tccagcgctt gatgatgcct tcgcgcggcg
     5401 gcgtaatggt gcccgcccat accgtctcaa tgctgcctac aatcgtcgca ccgaccagaa
     5461 tctgcgccat cgggccaaat tcggtatcga acaggcagat cacgcgttcg ttacgggcaa
     5521 acagattcgg cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt
     5581 agatcatttc acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgtgggg
     5641 agaggtaagt ggtcacaaac gtaccgttac ggaagagatc cgccatcaga tagttgcctg
     5701 ccagcagggc ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga
     5761 ttttacccaa ctggctgata acgccatcgg caggcataac caggacattc ggatcggtat
     5821 cgattgggcg tacttcatca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc
     5881 tggcggtgtc cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat
     5941 cgataaccag ttttgtcagc catcctgccc gcttgcttgc gccccaaccc gccaggcgag
     6001 taagccatag tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag
     6061 cctccaggcc attgttttgt cgttcctgat ccggcctaca tgccggatcc tgaaaaaaag
     6121 gggacgattc taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac
     6181 gcgtttttac ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg
     6241 cgattttccc ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc
     6301 aatcgcgata tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt
     6361 gttccggctc caggtgccag agaccaaact cacgcacacc tggggaatca atcacatcac
     6421 cgccgtgcgg gaagtgatac agtcgtgcgg cggtcgtggt gtgctgacca agccccgagt
     6481 tgtcagagac atcgttggtc aggatctctt tttgcagacc cagcaacgcg ttcagcaggc
     6541 tggatttgcc aacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt
     6601 ccagcggttt cagcccatcc tgagtatgac tggaaaccat caatacgcga taaccgatat
     6661 tgcggtagat atccatctgc tcgttgacga actccatgcc ttcgtcgtcc agcaggtcta
     6721 tcttgttgag cacaataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt
     6781 cgataatatt gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt
     6841 tggcagcaat aggtttcaca ccgtcgtaga aatccgggcg cgtcaacact gaggtgcgct
     6901 catgcaccgc ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac
     6961 gccagactac gcggtcgccg gtcaccaacg aacggatggt acggcgaata ttgcagcggt
     7021 gaacgtcgcc atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac
     7081 cttcatcagg ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa
     7141 gacgacgctg gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac
     7201 tcaatcgtac agactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg
     7261 tggatcaata tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc
     7321 tgatttggat cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga
     7381 ttgccacgct ggtgaccgat gccaacctga atattctggc agaagggccg accattgcag
     7441 tacatcagtc cgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg
     7501 ccagcgggct ggtagagcgt gtgaaagcga gcacgatggg cgatcgtgaa gctgaactgg
     7561 caacgctcga attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgtggtaaca
     7621 gcatcggtca ggaccgtcgc ttcctgttta aatacatgcc ggagctggaa gcctacttcc
     7681 actaccgcta tctcgatgtt agcaccctga aagagctggc gcgccgctgg aagccggaaa
     7741 ttcttgacgg ctttaccaag caggggacgc atcaggcgat ggatgatatc cgtgaatcgg
     7801 tggcggagtt ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac
     7861 gtaagcgccg gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgccttgctg
     7921 ataatttcag cacttgagat aaaaactcaa aaaaaatttt ttaggggggt tgcagaggga
     7981 aagatttctc gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca
     8041 aaattaagta gtacgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg
     8101 tcgcgagttc gagtctcgtt tcccgctcca aaatttgaaa agtgctgcaa agcacagacc
     8161 acccaagcgg gaatagctca gttggtagag cacgaccttg ccaaggtcgg ggtcgcgagt
     8221 tcgagtctcg tttcccgctc caaaatttga aagtgctgta aagcacagac cacccaagcg
     8281 ggaatagct
//
LOCUS       DAJOTB010000191         8197 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJOTB010000191 DAJOTB010000000
VERSION     DAJOTB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.191"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..759)
                     /locus_tag="OHN28_004333"
     CDS             complement(<1..759)
                     /locus_tag="OHN28_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HCQ2299611.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVFSYPAAAITLIGSGALRGAGNTKIPLLINGSLN"
     gene            764..839
                     /locus_tag="OHN28_004334"
     tRNA            764..839
                     /locus_tag="OHN28_004334"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:797..799,aa:Asn,seq:gtt)
     gene            complement(877..1827)
                     /gene="cbl"
                     /locus_tag="OHN28_004335"
     CDS             complement(877..1827)
                     /gene="cbl"
                     /locus_tag="OHN28_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCQ2299612.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1929..2846)
                     /gene="nac"
                     /locus_tag="OHN28_004336"
     CDS             complement(1929..2846)
                     /gene="nac"
                     /locus_tag="OHN28_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCQ2299613.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3173..3248
                     /locus_tag="OHN28_004337"
     tRNA            3173..3248
                     /locus_tag="OHN28_004337"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3206..3208,aa:Asn,seq:gtt)
     gene            complement(3304..4239)
                     /gene="ldtA"
                     /locus_tag="OHN28_004338"
     CDS             complement(3304..4239)
                     /gene="ldtA"
                     /locus_tag="OHN28_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2299614.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4301..5380)
                     /gene="cobT"
                     /locus_tag="OHN28_004339"
     CDS             complement(4301..5380)
                     /gene="cobT"
                     /locus_tag="OHN28_004339"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCQ2299615.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5392..6135)
                     /gene="cobS"
                     /locus_tag="OHN28_004340"
     CDS             complement(5392..6135)
                     /gene="cobS"
                     /locus_tag="OHN28_004340"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCQ2299616.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(6132..6677)
                     /gene="cobU"
                     /locus_tag="OHN28_004341"
     CDS             complement(6132..6677)
                     /gene="cobU"
                     /locus_tag="OHN28_004341"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCQ2299617.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2231 a         2158 c         1757 g         2051 t
ORIGIN      
        1 attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac gaagtgcccc
       61 gctaccaata agagtgatgg cagctgctgg ataactgaat acggtcagct ccagataagt
      121 caacgccagt gctttaacgt ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc
      181 gccaaaatga tgaattagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga
      241 ctgccgcgtc gccaccctcg ctcgtcgtcg atctcgctta ccgagactaa atgctacaac
      301 gacagtagta ccaagatcga tagctgcaaa aaaagccata atgaccatat tgaagctgtc
      361 cgccaacccc acgccggcca tcgcatcttt tcccagccag ctgaccagaa aagtgctcag
      421 gacccccatc aacaggacac aggcgttctc catgaagata ggaacagcaa gcggggttat
      481 ctcgcgccag aacaacactt tgtagctctt gcgtttagag tgccagcgag tgccgtgaac
      541 aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa agtgaaacca
      601 catttcaaat gatgagggag aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct
      661 gcaaatgcaa caaactgttg atagaaacgg caaacagttg ggaaatttaa aaatcgggtt
      721 tgacaaaata tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc
      781 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
      841 atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc
      901 ttcactgttt tccatcacct ggcgcttgat atcctcaact gacagtcctg cattacaaag
      961 ttccagaaag cgccagacat agttacgctg aagttgccct cgcttcagtc ccaaccagac
     1021 agtattagca tcgaaaagat gccgcgtatc caggcggatt aaattctttt cctcttgttc
     1081 gccgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat
     1141 gacatcagaa tcctgtgcac ttaatacaat atctgccggc aaacctttgc gggcaaatgc
     1201 gtcatcaatg cgtgagcgcc ccgtaatccc ctgtcggtaa gtaattaacg gccacttcgc
     1261 tattgattcc agcgtcaacg gtgtaatttg cgtcaaggga tgatcgagtg gaacaagcaa
     1321 actatggtgc caacgaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc
     1381 gctggcgata ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt cctgtggtgt
     1441 accctggatt agctccagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc
     1501 aggcaagcta taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc
     1561 gttggtaaac aggtctgcga gccgacgaac attactggct tcattcagaa tgcgttctgc
     1621 aatgaccagt aatgctttgc ccggttcagt catgcccagc agtcgcttac ctcgtcgaac
     1681 aaatatttcg atgccaagtt catcctccag ttcccgaata tgacggctaa cgcctgactg
     1741 tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcctcgcg
     1801 gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat atcccgttat
     1861 tgttaaagtc taatgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga
     1921 tataacgctt agctcaccaa ttgccactgc cttttttcca tcactggaga actaatcact
     1981 gacattaaca actctttcac tgcctgtgcc tgtggtgaca agtttgctct ggcgggtaaa
     2041 tttaaggaca aagagagact cattgaagga gtggtaatgc gtgacatcca cccatttact
     2101 gcgccacata acgaacgcgc agccgattca ggtaatactg caacgcccat gccgctggca
     2161 atcgctgcgg taagcgtggc aatagactca atttcaccaa tgacttttgc cgtgagccgc
     2221 cgtagggaaa aagcctcatc aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa
     2281 aggttcattt gcgcaatagc attaacatca acgctttgcc ccgggcaatc ttgagttcct
     2341 accaggaaaa gatcttcttt cagcaaagcc tgactggata ccccagccac aggggaatgc
     2401 tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag cactgcacca
     2461 ctattttcat gaagatagat aacgacctcc gggaattcag cgcgaaccgc ctgtaataag
     2521 ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac ttgccccgat
     2581 aatgactgac caacgttatg caccgccagt tgggcctgct cacactgacg taaaatggcc
     2641 cgtgcatggg tatagagaat ttttccggcg cctgttggcg taacgccccg ttttgtgcga
     2701 atcaaaagct gttgatttaa ctcaccttcc agtgtggcaa cctgctggct gagcgctggt
     2761 tgtgcgatat gcaatacttc agcagcctgg gtcaggctac caatatctac aatttttacg
     2821 aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttaagaatcg gcccaagtgc
     2881 cgccattact tacaaccaga atgcaagatg cttgccagtt ctattttagc gttgatgcgc
     2941 aagctaacca actgtcaaat aagagattat gatagattcg tcattggccc tttttatcag
     3001 cagtcgcatt cacctgccct ataaaagggg ggtatgcacc acgatggttc attacccaat
     3061 aagattgaaa gctcaccact ttgttgaaat tgtcagcaaa caaacaaaaa aatgcatttc
     3121 accctttgac atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt
     3181 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     3241 aggagccaaa ttcaaaaaag cctgctttcg agcaggcttt ttactttcta attaccaacg
     3301 ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgatgttg
     3361 caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga
     3421 tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag
     3481 cggctcatga acttccagcc actttgaacc atccggttcc gttgtgtatt tcactggctg
     3541 atcaataatt tgtacacgag tgcctacagg aacattatca aacagatatt tgatatcgtc
     3601 attacgcaga cgaatacagc cctgacttac gcggagccca ataccaaaat tggcattggt
     3661 accgtggatg gcatacaact tgccaatata aatcgcgtac agccccatgg ggttatcggg
     3721 ccccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgggtgtt
     3781 cggcgttggc gtccaggttg gtgcttcttg tttacgttca acggtagtca cccagttacg
     3841 cggggtttct cgcccagcct ggccgatacc aataggaaag acttccactg tattactgtc
     3901 tggcggatag taataaagac gcatttcagc gacgttaacc acaatccctt tacgaacagt
     3961 ggcgggcaaa atcagttgct gcggaatggt gagttgtgag ccaggcttcg gcaaaaaaac
     4021 atcagcgccc ggattcgctt ccagcatatt acttaaccct tgcccgtatt gtgcggcaaa
     4081 agtctccagc ggctgagtat tgtgatcagg aacagttaca gtaagcgact gccccactaa
     4141 acggctaccc tctggaggta atggataagt taccgccagg ctattttggc tggcaaaaag
     4201 cagagcaaat gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa
     4261 tactatccga taagtatagc ttttatcaga cttttcgttt ttagctgttc aaatcagaag
     4321 tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatgttg ttgtatatcg
     4381 cacaagcagc ttcgatgatg gacatcgcca gagctgcacc actcccctca cctaaacgca
     4441 tatccatatt gagataaggc tccagcccca aatgcgagag cgctatacgc gcgccttttt
     4501 ctgccgacaa gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg
     4561 cgagcgcagc agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag
     4621 cacctaacat cactccagct attccgacca aatcaaatcc acccactttt gccaggacat
     4681 caacaccatc ctgaggattt ggttgattca acgtaatcgc ccgacgcaca acatcaattt
     4741 tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc
     4801 ggccagtgat tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac
     4861 ctacaccaaa cagcgtgaca ccgttttttg ccagctcccg cgtataacat atgacgtcca
     4921 aaagcaactt ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac
     4981 ctcgtgcgac acgcatgttg ataagcccgg ggataggctc agcagtatca ataccaacat
     5041 caattacgtg gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccac
     5101 gggtcatatt ttcagcctgt atggctgtca cttcttttgg agaaatagcg accccttcct
     5161 cccagacgcc gtgatcggca cacataacca gtactgcttt tttgcccaca tgcggtatgc
     5221 cattcaaccc cggcattcct gccagttgta tggcaagcgc ctccagcttt cccaggctac
     5281 caacaggttt gagtaacccg tcaacatgcc gttgtgcacg cgacatagcg gcagaattga
     5341 tggctgggat cgtattcagt aaatcggcaa gtatttgcat gtcacgtctc tttatagcag
     5401 tgccagcagg aaaaccagtt caccaaactc tatggctgct cccagcgtat cgcccgtttg
     5461 cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac
     5521 catagcagcc acaccatgca tacccggcaa taatacggca gcaaaaatag cggctaagcc
     5581 gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc
     5641 ttcccgcgca taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca
     5701 tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaacaccag
     5761 aatctttgcc agtaccacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc
     5821 acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc
     5881 agcgaggccg tccagatgga atcccccggt catcagcgcc agcacaagta cgctaaacag
     5941 tgccgccagt ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc
     6001 gccaagcaat aatccaatca aaggaaaagt aataataccg cgagaataat gctcgaaatc
     6061 cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaatgaga gcatcgccca
     6121 aaataattta ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt
     6181 gccgcagcgg ccaactgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga
     6241 cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg
     6301 caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc
     6361 tgataatccc attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc
     6421 attgtggtaa cgcattccag caacacagcc tcattcgggt taatgtctgc atgaattaat
     6481 tcatcaagat gttgccagcg ctccactgtg cgccagtgct ccgggcggct ttgccgatga
     6541 tgttctatcc gtgcagccat ctcatcatca aggatttgcg aggtagcgat atacagaacc
     6601 tgtgaagagt ccccaataag cacctctgcg tggcgactct tcccgctccg tgcgcctccc
     6661 gtcaccaaaa tcatcatacc gactcctgat tcaaatgtat gatggtattc attgaacaga
     6721 gcaatggatc ccccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa
     6781 tataattaaa aatccagcat tctcaatcca ttacgaaata agatggcatt acggaatagc
     6841 cagactttct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctgtggattg
     6901 acacatttca ttcagataat gaattaatgc tactgccgga ccaaccagca aacgggcatt
     6961 gtgttctgaa atccaggcta ttgattcaac gtcagcgaca gatctgcgct gtaaaagaag
     7021 ttgtaaaaca acgcaataaa attatccgga atggtggcga ctatgcacta gaacaccaca
     7081 atttcgctct ctcggtaagg cactgtcaca gcattcaaca gaatgtgact tgccagattt
     7141 attagcgcca ccagatgttt aaccgggtaa ccacgaccca gtcggggata tattccatct
     7201 tctcgctgac atcatcaaga ataaaaaggt ttatcacact cagagcaacg ttcttcagca
     7261 ggcgggataa ttcttcaaca tttaaaagat gtgttattaa aggcatacaa actggacgta
     7321 ttgtattttc ttttggtggt tgacctaagg tagcagttta tcctgatgcg ctgagatttc
     7381 tgtaatatct acgtcaacat tctcctcgaa tagaaaatgc agcctcggca agtgattcac
     7441 attttttaac aaagcgttgt tagcgtgcca ttttcagaag ctctccaaga aagcgaattc
     7501 ttttctcaaa ttctgcattc tcagacacag ctttctactc catggcaatg gcccaaatac
     7561 gaagttgctc aggatcatta gtggtgctga gaagttaaat aaccatgcgg agtacaactc
     7621 gtcatcacgt atttatatat catgaagcta ttgttgtgtt ttgtacatca gtagatgtat
     7681 tctgatatac tcctttttct agacataacc tttcacctgc ttgcaaagct tctgtgttct
     7741 gacattgcca aattgttgca attctgtatc cagccttctt tcagtcatag cttcgggccg
     7801 cgataagact cactgatctg accctgattc ctcttgcaga ctttatagac caattaaaat
     7861 gcagtttctg caggtcaacg tctgaccatc attgtcatca ctctggccat tagagtaacc
     7921 ttctgcattc atccttttgt aaaaagttta tgtcagtatc agcaattaac cggacctgat
     7981 actgatatga gtcttaccgc atatacggtc aatttcagca attaattaca ttatccacgc
     8041 caaagtgttt gtcatcgcaa tgatggtacc ttctttcaga caccattttt tcaactccgt
     8101 tttccacgga ccgcactctt atgtcaagag tacggtccgt ggatacaacc agagaccgac
     8161 tgacacgagt cagaggaaac gacggatatg ttcagtc
//
LOCUS       DAJOTB010000192         8179 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJOTB010000192 DAJOTB010000000
VERSION     DAJOTB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.192"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            33..1199
                     /locus_tag="OHN28_004342"
     CDS             33..1199
                     /locus_tag="OHN28_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCQ2299618.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1242..2558
                     /locus_tag="OHN28_004343"
     CDS             1242..2558
                     /locus_tag="OHN28_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2299619.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2662..3312
                     /gene="fabR"
                     /locus_tag="OHN28_004344"
     CDS             2662..3312
                     /gene="fabR"
                     /locus_tag="OHN28_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCQ2299620.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3312..3671
                     /locus_tag="OHN28_004345"
     CDS             3312..3671
                     /locus_tag="OHN28_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCQ2299621.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(3711..4811)
                     /gene="trmA"
                     /locus_tag="OHN28_004346"
     CDS             complement(3711..4811)
                     /gene="trmA"
                     /locus_tag="OHN28_004346"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCQ2299622.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4925..5115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5180..7024
                     /gene="btuB"
                     /locus_tag="OHN28_004347"
     CDS             5180..7024
                     /gene="btuB"
                     /locus_tag="OHN28_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCQ2299623.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6969..7826
                     /gene="murI"
                     /locus_tag="OHN28_004348"
     CDS             6969..7826
                     /gene="murI"
                     /locus_tag="OHN28_004348"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCQ2299624.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         1881 a         1929 c         2215 g         2154 t
ORIGIN      
        1 agcctgttta taagtgaata aaggaatagc cgatgatcga acactatatt cgtgggtttg
       61 aggaagaact tcgcgagatc cgccatcaga tccacgaaaa cccggagctg ggattacaag
      121 aatttaaaac cagtgctttg gttgcggaaa agctgcgcca gtggggctat gaggttgagc
      181 aagggctggc gacaaccggc gtggtagcca cgttaaaagt gggcgacgga gagaaaagta
      241 ttggcctgcg ggcggatatg gatgcgttgc cgatttatga aaatagtggc aagccctggg
      301 ccagtaaaca tcctggttta atgcacgcct gcgggcatga cgggcatacc accatactgc
      361 tgggggccgc gcgttatttt gccgaaaccc gccgttttaa cggcacgtta cgccttatct
      421 tccagccagc ggaagagatg attaacggcg gtgagatcat ggttaaagag gggctttttg
      481 atcgtttccc ctgcgatgtc attttcggga tgcacaacat gccgggtttg ccggtcggca
      541 agtttttctt ccagcctggg gcgctgatgg cgtcaatgga tcagttccat attacggttc
      601 gtggttgtgg cggacacggt gcgatcccgc acaaagccat cgatccagtg ctggttgccg
      661 cacatatcac caccgcatta caaagcattg tgtcgcgcaa tgtcgatccg ctggaagcgg
      721 cggtaattac cgtcggcagc attgttgcgg gtgaggcggc taacgtcatt cctgacagtg
      781 ctgagatgaa aatcagtgtc cgctctctta gccgcgatac ccgacaactt cttctgaccc
      841 gtattcctgc tcttgcacaa acccaggctg ccagctttgg cgctacggct gaggttacgc
      901 atgttaacgg tacaccggta ttagtaaatg acgaggagat ggcgcgcttc gcctggcagg
      961 tggcgtgcaa aacatttggt gaagatcggg ctgagtttgg catcaaaccc ctgatgggca
     1021 gtgaagattt ctctttcatg ctggaggccc aacccaaagg cggtttcctg ttattcggta
     1081 atggcgatgt cggggaaggt tcctgcatgg tgcataaccc cggttacgac tttaacgatg
     1141 caagcctggt cccggcaagc agctactggg gcgcactggt tgaagcctgg ctgcaataaa
     1201 taaaacaaca cacaacaaca ctttttggca aggaacatgg aatgacggcg atcgtaaatg
     1261 aaaccgtgag tgtgaagaaa aagcccagcg gcaggcgggt gatctttgcc tcggcgtttg
     1321 gcaatgcgct cgaatttttt gattttggcg tttacaactt ttttgtggtc tatatcagta
     1381 cgcttttctt cccacccagt gccgatcata acgtcgcgct gttactggca tttgcaacgt
     1441 ttggtgtgag cttttttatg cgtccgctcg gtgggattat cgttggtgcc tgggccgatc
     1501 gcttcgggcg taaaccggcg atggtgttca ctattgctct gatgagcctc ggtacgctga
     1561 tgatcggcat cgcaccgacg tatgaaacgg cgggctattg gggaaccgca acgctggtgc
     1621 tggcgcgttt gattcagggc gttgcggcag gtggtgaagt cggggcttca atgtcgctac
     1681 tggtggagtc agctccggca aatcgtcgtg gcttttacag cagttggtca ctggcgactc
     1741 aaggcctggc aacaactttt ggcggtgttg tcgctttagg tttaagcgca tggctaccgt
     1801 tcgccacagg ttcagaaacc gtaatggcag aatggggctg gcgtgtgccg ttttttattg
     1861 gtgtgttatt agcacctatc ggttgctggt tgcgtctgag cctggaaaat gacgtacctg
     1921 agcctgtgcg caataaaaaa gctgctacca gcgaaagcgc gttttctctg ctcctgcaac
     1981 ataaggcgac tatcgttaat ggcgttttac tcgctattgg tagcacggtc gcaacctata
     2041 tctccctctt ttattacggc acctgggcgg cgaagtatct ggcgatgcct caacattatt
     2101 ctcatgcagc gatgttactg gcgggcgttg ttacgtttgt gggtgcgctg cttgtgggga
     2161 tgttgtgcga ttcggtcggg cgtaaaaagc tgattttaat ctcccgtgtg atggtgcttc
     2221 tctgtagctg gccgtcattc tggctgttgg tgaattaccc aagccctggc atgttgctga
     2281 cggtggtttt cgtgatggtc agctttacca cgcttggcgg tgtgccagtg atgttgttga
     2341 tctctgaact gttgccgaaa cggattcggg cattgggttt tgcgctggtt tatagcattg
     2401 gtgtggcaat ctttggcggt tttgctcaat atttcgctac gcaatccatc gtgttgctgg
     2461 atagtctgac ggctccggcg tggtatctgg gtggaggcac gttgctgtcg atgctggcgt
     2521 tgttgtacgt aaaagaaccg gccaaagaat tgcagtaaat atgttttatt gcgttaccgt
     2581 tcattcacaa cactggagca atccagtatg ttcattctct ggtatagtgc cagcagtact
     2641 tttggcaagg attcagacat cgtgatgggc gtaagagcgc aacaaaaaga aaaaacccgc
     2701 cgttcgctgg tggaagccgc atttagccaa ttaagtgctg aacgcagctt cgccagcctg
     2761 agtttgcgtg aagtggcacg agaagcaggc attgctccca cctcttttta tcggcatttc
     2821 cgcgacgtag acgaactggg tctgaccatg gttgatgaga gcggtttaat gctacgccaa
     2881 ctcatgcgtc aggcgcgtca gcgtatcgcc aaaggcggga gtgtgatccg cacctcggtc
     2941 tccacattta tggagttcat cggtaataat cctaacgcct tccggttatt attgcgggaa
     3001 cgctccggca cctccgctgc gtttcgtgcc gccgttgcgc gtgaaattca gcacttcatt
     3061 gcggaacttg cggactatct ggaactcgaa aaccatatgc cgcgtgcgtt tactgaagcg
     3121 caagccgaag caatggtgac gattgtcttc agtgcggggg ccgaggcgtt ggatgtcggc
     3181 gtcgagcaac gtcggcaatt agaagagcga ctggtactgc agctgcgaat gatttcgaaa
     3241 ggggcttatt actggtatcg ccgtgaacaa gagaaaaccg caattattcc gggaaatgtg
     3301 aaggacgagt aatgaaacaa gcaaatcaag atagaggtac gctgctgctg gcgctagtgg
     3361 ctggcttatc gattaatggt actttcgcag cgctgtttag ctccattgta ccattttctg
     3421 tattcccgat tatttccctg gtgttgacag tttactgcct gcatcaacgt tatcttaatc
     3481 gcactatgcc ggtaggcttg ccgggtctgg cagctgcttg ttttattctc ggcgtactgc
     3541 tgtacagcac ggtagttcgt gcggaatatc cggatatcgg ttccaacttc ttcccggcag
     3601 tactgtccgt cattatggtg ttctggattg gcgcgaagat gcgtaaccgt aagcaggacg
     3661 ttgctgagta atcagcatca tgccggatgc aggcgcatcc gacatgggtt ttacttcgcg
     3721 gtcagtaata cgccgcactc catatggtgc gtgtagggga actgatcaaa tagagccaga
     3781 cgttcgacct tgtgcgtctg gcttaatgtt tccagattct tgcataacgt ttccggattg
     3841 caggagatat acagaatacg tggatacgcc tgcaccattt tctcggtttc actgtccaga
     3901 ccgctgcgcg gtgggtcgac aaaaatggtt tcgcactgat aactctttaa gtcgattcct
     3961 tgcaggcggt taaactcgcg cacaccattc atcgcctgag taaattcttc tgccgccata
     4021 cgaataattt gtacgttatc aatatggtta gcggcaatgt tgtattgcgc ggcggcgact
     4081 gacggcttgg cgatttcggt ggctaatacc cgatcaaaat tgcgtgccag cgctaatgaa
     4141 aagttaccgt tgccgcagta tagctccagt aaatcgcctt ttgagccttt ggttacgtcc
     4201 agcgcccatt ccagcatctg aatattcatc gccgcgttcg gttgggtaaa gctgttttct
     4261 acctgacggt agatcatctc tttccccgcg atcggcagac gttcgtcgat gtaatcctga
     4321 tccagcgcga ttttggtttt cgttgcccga ccaatcagat gcacattcag attctgcgcg
     4381 cgcagtgcat cgcgcagggc ttccgcctgc tgacaccact catcatccag ctttttatgg
     4441 tacagcaagg aaacaaccgc ttgattgctt agcgtagtga ggtaatcaat ctggaacaac
     4501 ttgtggcgca gaatgggatt attacgcaca cccgcaatca tcgccgtcat caactggttg
     4561 ataagttcac tggcggcggg gaagctatcc acacggatac gacttttggt ttgttgatcg
     4621 aaaatgatgt gatacaggtc atcgccatcg tgccagatgc ggaactccgc acgcatccga
     4681 taatgactga ccggcgagcg aaacacttcc ggaaccaggt cagaaaacgg tgccatcata
     4741 ctttgcaaac gtaccacttt ttcggctaac tgcgcttcat actgttctgt tggaaggtgt
     4801 tcgggggtca tgatgtatcc tgaaaaatta aagtacgcgg cgattgtagg gattgctcat
     4861 cagatgtcca gatcttgatg aattcctatt tgtgagctac gtctggacag taacttgtta
     4921 caacctgtag catccacttg ccggtcctgt gagttaatag ggaatccagt gcgaatctgg
     4981 agctgacgcg cagcggtaag gaaaggtgcg atgattgcgt tatgcggaca ctgccatccg
     5041 gtgggaagtc atcatctctt agtatcttag atacccctcc aagcccgaag acctgccggc
     5101 caacgtcgca tctggttctc atcatcgcgt aatattgatg aaacctgcgg catccttctt
     5161 ctattgtgga tgctttacaa tgattaaaaa agcttcgctg ctgacggcgt gttccgtcac
     5221 ggcattttcc gcttgggcac aggataccag cccggatact ctcgtcgtta ctgctaaccg
     5281 ttttgaacaa ccgcgcagca ctgtgcttgc gccaactact gttgtgacgc gtcaggatat
     5341 cgaccgctgg cagtcgacct cggtcaatga tgtgctgcgc cgtcttccgg gcgtggatat
     5401 cacccaaaac ggcggttcag gtcagctctc atctattttt attcgcggta caaatgccag
     5461 tcatgtgttg gtgttaattg atggcgtccg cctgaatttg gcgggggtga gtggttctgc
     5521 tgatcttagt cagttcccta ttgcgcttgt ccagcgtgtt gaatatatcc gtgggccgcg
     5581 ctccgctgtt tatggttcag atgcaatagg cggggtcgtg aatatcatca cgacgcgcga
     5641 tcaccccgga acggaaattt cagcagggtg gggaagcaat agttatcaga actatgatgt
     5701 ctctacacag caacaactgg gggataagac acgggtaacg ctgttgggcg attatgccca
     5761 tactcatggt tatgatgttg tcgcctatgg taataccgga acgcaagcgc agccagataa
     5821 cgatggtttt ttaagtaaaa cgctttatgg cgcgctggag cataatttta ctgatgtctg
     5881 gagcggcttt gtgcgcggct atggctatga taatcgtacc aattatgacg catattattc
     5941 tcccggctcg ccactggtcg atacccgtaa actctatagc caaagttggg atgccggact
     6001 gcgctataac ggtgaactga ttaaatcaca actgattacc agctacagcc atagcaaaga
     6061 ttacaactac gatccgcatt atggtcgtta tgattcatcc gcgacgctcg atgagatgaa
     6121 acagtacacc gtccagtggg caaacaacat catcattggc cacggtaata ttggtgcggg
     6181 tgttgactgg cagaagcaga gcacggcacc gggcacagct tatgttgagg atggatatga
     6241 tcaacgtaat accggcatct atctgaccgg gctgcaacaa gttggcgatt ttacctttga
     6301 aggcgcagca cgtagcgacg ataactcaca gtttggtcgt catggcacct ggcaaaccag
     6361 cgccggttgg gaatttatcg aaggttatcg ctttattgct tcctacggga catcttataa
     6421 ggcaccaaat cttggacaac tgtatggttt ctacggaaat ccgaatctcg accctgagaa
     6481 aagcaaacag tgggaaggcg cgtttgaagg cttaactgct ggggtgaact ggcgtatttc
     6541 cggatatcgt aacgatgtca gtgacttgat cgattatgat gatcacaccc tgaaatatta
     6601 caacgaaggg aaagcgcgga ttaagggtgt cgaggcgacc gccaattttg ataccggacc
     6661 actgacgcat actgtgagtt atgattatgt tgatgcgcgc aatgcgatta ctgacacgcc
     6721 gttgttacgc cgtgctaaac agcaggtgaa ataccagctc gactggcagt tgtatgactt
     6781 cgactggggg attacttatc agtacttagg ttctcgttat gataaggatt actcctctta
     6841 tccgtatcaa acagtgaaaa tgggcggggt gagcttgtgg gatcttgctg ttgcatatcc
     6901 ggtcacctct cacctgacag ttcgtggtaa aatagccaac ctgttcgaca aagattacga
     6961 gacagtctat ggctaccaaa ctgcaggacg ggaatacacc ttgtctggca gctacacctt
     7021 ctgaaccacg tcccaccgtg ctggtgtttg actccggcgt cggtgggttg tcggtctatg
     7081 acgagatccg gcatctcttg ccggatctcc attacattta tgctttcgat aacgtcgctt
     7141 tcccgtatgg cgaaaaaagc gaagtgttta ttgttgagcg agtggtggaa attgtcaccg
     7201 cagtgcaaga acgttatccc cttgcgctgg ctgtggtcgc ttgcaacacc gccagtaccg
     7261 tttcacttcc tgcattacgc gaaaagttcg acttcccggt tgttggtgtc gtgccggcga
     7321 ttaaacctgc tgcacgtctg acggcaaatg gcattgtcgg attactggca acccgcggaa
     7381 ctgttaaacg ttcttatact catgagctga tcgcgcgttt cgctaatgaa tgccagatag
     7441 aaatgctggg ctcggcagag atggttgagt tggctgaagc gaagctgcat ggcgaagatg
     7501 tttctctgga tgcgctaaaa cgtatcctac gcccgtggtt aagaatgaaa gagccgccag
     7561 atacagttgt attgggttgc acccatttcc ctctactaca agaagaacta ttacaagtgc
     7621 tgccagaggg aacccggctg gtggattctg gcgcagcgat tgctcgccga acggcctggt
     7681 tgttagagca tgaagccccg gatgcaaaat ctgccgatgc gaatattgca ttttgtatgg
     7741 caatgacgcc agaagctgaa caattattgc ccgttttaca gcgttacggc ttcgaaacgc
     7801 tcgaaaaact ggcagtttta gattgatttg gttgaatgtt gcgcggtcag aaaattattt
     7861 taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac
     7921 aacggcaaac acgccgccgg gtcagcgggg ttctcctgag aatctcaaca gaggaaagca
     7981 aaaaaatgct tgactctgta gcgggaaagc gtattatgta taccccgcgc cgctgagaaa
     8041 agcgaagcgg cactgctctt taacaattta tcagacaatc tgtgtgggca ctcgaagata
     8101 cggattcttg acgtcgcaag acgaaaaatg aataccaagt ctctgagtga acacgtaatt
     8161 cattacgaag tttaattca
//
LOCUS       DAJOTB010000193         8090 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJOTB010000193 DAJOTB010000000
VERSION     DAJOTB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.193"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            169..2412
                     /gene="pdeF"
                     /locus_tag="OHN28_004349"
     CDS             169..2412
                     /gene="pdeF"
                     /locus_tag="OHN28_004349"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCQ2299625.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2451..3992)
                     /gene="ppx"
                     /locus_tag="OHN28_004350"
     CDS             complement(2451..3992)
                     /gene="ppx"
                     /locus_tag="OHN28_004350"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCQ2299626.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGTDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3997..6063)
                     /gene="ppk1"
                     /locus_tag="OHN28_004351"
     CDS             complement(3997..6063)
                     /gene="ppk1"
                     /locus_tag="OHN28_004351"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCQ2299627.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6235..6873)
                     /gene="purN"
                     /locus_tag="OHN28_004352"
     CDS             complement(6235..6873)
                     /gene="purN"
                     /locus_tag="OHN28_004352"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCQ2299628.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6873..7910)
                     /gene="purM"
                     /locus_tag="OHN28_004353"
     CDS             complement(6873..7910)
                     /gene="purM"
                     /locus_tag="OHN28_004353"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCQ2299629.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         1974 a         2016 c         1975 g         2125 t
ORIGIN      
        1 ataattcatt aatcagggtt attttgtttg atatatatca attggaactt tcatatgaca
       61 cttagaatca ttgctctcta tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc
      121 tgtctgaata cacctccttc aggatgtggg ggattctgct gagcatctat gaaactgaat
      181 gcaacttata taaaaatacg tgataaatgg tgggggcttc cgctgttcct gccttcttta
      241 atcttgccca ttttcgccca cattaatact ttcgcgcata tttcttccgg tgaagttttt
      301 ctcttttatc tgccactggc actgatgatc agcatgatga tgtttttcag ctgggcggca
      361 ttgccaggga tcgccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa
      421 acgctctcat taacggctaa ttttattatc attatcattc tctgttgggg cggttacagg
      481 gtttttaccc cccggcgtaa caacgtttca catggtgata gccgtttaat ttcccagcga
      541 ctattctggc agattgtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgcg
      601 tttgtagggt tactggcgag cagagaaaat ctggtcggcg tcatgccctt taacctcggg
      661 accttaatca attatcaggc cttgctggtg ggtaatctga ttggtgtccc gctgtgctac
      721 tttatcattc gggtggtgcg aaatccgttt tatttacgta gctattattc gcaattaaaa
      781 cagcaggttg atgccaaagt taccaaaaaa gagttcgcaa tctggctact ggcattaggt
      841 gccttattgt tgctgttatg catgccgtta aatgaaaaaa gcacgatttt tagcaccaat
      901 tacaccttgt cattattgct gcccctgatg atgtggggag cgatgcgcta tggttataag
      961 ttgatttcat tgctctgggc ggtcgtgttg atgatcagca tccatagcta tcaaaattac
     1021 attcccattt atcctggcta taccacgcag ctgaccataa cctcctccag ttatctggta
     1081 ttctctttta ttgtcaatta tatggctgta ctggcaaccc gtcagcgagc tgtagtcaga
     1141 cgcattcagc ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg
     1201 aatcgcgcgt tgcgtgatgc cccctggtct gcgctatgct atttacacat ccctggcatg
     1261 gaaatgctgg ttaagaacta tggcatcatg ctgcggattc aatacaagca aaaactttct
     1321 cactggctgt caccattgct ggaaccgggt gaagatgttt atcagctttc gggtaacgat
     1381 ctcgcgctgc ggctgaatac agaatcgcac caggagcgca ttaccgcact ggatagccat
     1441 ctcaagcaat ttcgtttctt ttgggatgga atgccgatgc aaccgcagat tggcgtcagt
     1501 tactgctatg tgcgctcgcc agtgaatcat atctacctgc tgctgggaga gctaaatacg
     1561 gtggccgaac tttccatcgt gaccaacgtc ccggaaaata tgcagcgtcg cggagcaatg
     1621 tatttgcaac gcgaattgaa agataaagtc gcgatgatga atcgactaca gcaggcgctg
     1681 gaacacaacc attttttcct gatggcccag ccgattaccg gtatgcgtgg tgatgtttac
     1741 catgaaattc ttctgcgcat gaagggtgag aatgatgagc tgatcagccc cgatagcttc
     1801 ttaccggtcg cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt cattgagcat
     1861 acgctgcaat ttatggctga aaacagagcg aagatgcccg ctcaccgttt tgctattaat
     1921 ctgtctccaa cctcggtatg tcaggctcgt tttcctgttg aagtcagtca gctgctggct
     1981 aaatatcaga ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc
     2041 aatgttaagc aggcgcaaat caccttgcag catcttcagg aattaggctg ccagattgcg
     2101 attgatgatt tcggcaccgg ctacgccagc tatgcgcggc ttaaaaatgt gaatgccgat
     2161 ctgcttaaaa ttgacggcag ttttatccgc aatattgtgt caaatagtct ggattatcag
     2221 atagtggcgt cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac
     2281 gttgaaaacg aagagatccg cgaggcggtg ctctctttgg ggatcgatta tatgcagggt
     2341 tatcttattg gtaagccgca accgttaatt gatacgctga atgaaatcga acccattcgc
     2401 gaaagtgcct gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg
     2461 atttctggtg tactttcttc ttcaattttc aaccgccagc cagccacgcc ttcccagtat
     2521 tcttgctcct tttccagatc aagcagtacc agcgcattct gactaaacca gtcatgcggg
     2581 aaacgcagtg tccagtgact gtcatcagta atcagcgtca atgttggcgg tgtggtggtt
     2641 gcctgacgtt gattattgag taatacgcca aggcgcaata gctgaatcag tggcaggaac
     2701 tgtttcttct taaacaaggt aaagcgcggc agatcgtcga gtttaatcgc tttacggtga
     2761 tagcgcacca gtgtcgccat catcagctgc tgttcctgat taaaacccgg caagtcgctg
     2821 ttttgcagaa tataagcgga gtggcgatgc aaaccgctgt ggttgatatt caacccgacc
     2881 tcatgcagca tggcggccca tcgcagtagc gcctccagtt gtggatgcgc cagcttcggt
     2941 tgctgttccc gccactgttc gtacatttgc atagtggtat ccagcacccg tcgggcctgt
     3001 tcgctgtcga tgtgatactg gttggcgagg ctgctggcag tgcgactacg cacatcctga
     3061 tgacggaaac gtccttccat ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg
     3121 cgcagttcac ggatggctaa agcatcaaac acaccgcata aaatcgccag tcccggaacg
     3181 aagactgttt tccgttcttc ggaaagaccc ggtaaactca gcgatgcgaa attacggtga
     3241 cgtaaaactt cttttaccag tttttccaga cgttccgggg taattatccc gtctttctcg
     3301 cccatttcca ttaacacttc atgggcggct tttatggtac cggaagcgcc cattgccacg
     3361 ttccagccct gaattcgaaa ttgccaggtt aacgtttcca gtttttgtgc tgccgccatg
     3421 cgagcgcgct gaaaattctc tttattgatg accccgccag ggaaataaag ctgggcaaag
     3481 ctgacacaac ccatccggcg gctttcaacg agaatcggtt cgaaattttc accaatcacc
     3541 agttctgtag aaccgccgcc aatatcaata accagtttgc gacctttttc cggttgggta
     3601 tgttccacgc ccataaaaat cagacgggct tcttcattac cggaaataat ttcgatcggg
     3661 tagggaatga ctttttccgc gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagc
     3721 gtatgggtac caactataca gacgctggca ggggaaaacc cttgtagcct ttcggcaaat
     3781 agcgacagac agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt atctgtcccc
     3841 aggccgtccg ccagatgcac ccgctgtttc aggcggccaa taatctgcat ggcaccatct
     3901 accacacggg ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc
     3961 tgcggacgag gggatttatc gtgtattggc atagggttat tcagattgtt cgagtgattt
     4021 gatgtagtcg taaatcgcca actgcgcccg tactttgcgg cgattgccgc ggggaacgta
     4081 gcgattactg agttctttat cgatataacg tgctttgacc gtatcgctga acaatatgtc
     4141 gatgatgtcc agcacccgct gcttcaggcg cggatcgagc agcggcgttg ccacttcaat
     4201 acgataatca atattgcgcg tcatccagtc ggcggaagaa aggtagacct ttttatcgcc
     4261 gccattttca aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc
     4321 acgaatgttg tcgctaatgc cttccagatt ggggatcaga gaacacattc cgcgcaccag
     4381 cagattaacc ggtacgccgg agctggaggc cgcatacaga cgatcaacca gacctttatc
     4441 gacaaggtta tttagcttca gggtgatacc actgggcagc ccttgctgcg cgttggcgat
     4501 ctcgcggtcc accatttcat acaacagacg gcgagagttt tgcggcgaca ccattaaata
     4561 atcaaatgtc accggacggt atgggttttc aataaagttg aacacccgcc gtacttcgtt
     4621 agtgatgcgc gcatcggcgg tcagcaacga atagtcagta taaagacgcg cggttttttc
     4681 gttaaagttc ccggtcccga tgtgtgcgta acgcaccact tcaccgtttt ctttacgtga
     4741 aatcaggaac agtttggcgt gaattttcag ccccggcgca gagaagataa cgtgcacgcc
     4801 tgcttcggtc aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac gtgcctgtaa
     4861 ctcaaccacc acggtgactt tcttaccgtt gtgtgcggcg tggatcatcg agtcgatgat
     4921 gcgtgaatct ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa
     4981 cgaagcctga cgcagcagtt ccagtacatg ctcaaaggta tgataaggat aatagagcag
     5041 cacatcgcgc tcacgaatgg catcaaaacc gttgcggaac tgggctttat caaaccaaat
     5101 gtggcgtaaa cgtggcagtg gtttgttcac cagattggct ttgccgacat tggggaaatt
     5161 aataaagtct ttaaaattat gataacgacc gccggggacg atggagtcgt agcgggaaat
     5221 agtcagtttt tcgcgtaaca cttcaaccag cgcattgggc atatcgcgct gataaacaaa
     5281 acgcaccggc tcagcagtta aacgctgctt gagactggaa gacatcaact ccatcaggct
     5341 ggcttccatc tcatgcacta aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc
     5401 attcagcgcg tcataatcaa agaagccttt gaaaatatca tcaaggcagt aacgcagaat
     5461 gttatccaga agaatcatcg gcttgcgtcg acgcggcgct tctggcggta aattcacaaa
     5521 gcgcggcact ttatctgatg ggatctccag cagcgcgtaa cggatggtat cgccacggat
     5581 aatttccacc gccagatagg tgtaatcatc tttcaggaac tgcactaaat cagtgtcagg
     5641 attgattaaa atcggcgtaa tgtgctgacg cagatactgc ttaaaataat gacgcagcca
     5701 gttttgttga ttgacggaga gctggcgttc attaatcagg aagatctggt tgcgcgccat
     5761 ctctagcaac aactcgttgt agaggccgtc gaattcctga tcggctttca gcacccggga
     5821 ctgaattttg cccagtaaat ggcgggaatg agagttggaa ccttgttctt cgctaataat
     5881 gatgcgtcgc ttcagttcag cgaagcggac tttatagaac tcatcaaggt tattggaata
     5941 gatccccagg aaacgcatcc tttcaatcag cgggttagat ttgtccgccg cttcctgaag
     6001 cacgcgttca ttgaacgata accaactgag ctctttttcg atgtatagct tttcctgacc
     6061 cattaccact tttactccgt tttattcacg ggacactgcc tggatattat ggcgagcaat
     6121 attggcagat gtccaactat gccagaaaag ggggaaaaaa actgctggag cttgcgattt
     6181 acaggtgaat tgaaaaacgc cagcggcaga gctggcgctt taactacggg ggcattactc
     6241 gtcggcagcg tagccctgcg gcggcagacg ttgaccatcc agccacgcgg cgttttcgtg
     6301 cattttcaga cgaccatcgg caaaccagct aatcaccagt ggataaatag cgtgttcctg
     6361 ggtttgcacg cgggcggtga tgtcatcttc cgtatcacca gcaaataccg ggactttcgc
     6421 ctgtaaaata accgggccac cgtccagttc atcggtgacg aaatgcaccg atgtaccgtg
     6481 ctcttcatcg ccattttcca gcgcctgacg atgggtgtgt aatccgggat atttcggcag
     6541 cagagaaggg tgaatgttca gcaaacgccc agcatagtgg gagacaaacg ccgggctgag
     6601 aatgcgcata aaaccagcca gcacgaccac atcgggtgcg tacatgtcga tttcatgaat
     6661 caactcccgg tcataggctt cacgactgtc aaacgcgctg gtgatgagcg tatgcgttgc
     6721 aatacccgcc tggcgggcgc gttcaaggcc gaacgcgtcg gccttattgc tgaaaactgc
     6781 ccgtacggtg cctttaattt tgttggtttt gcaggcgtca ataattgcct gcaaattact
     6841 tccgttgccg gaaataagca ccacaatatt cattattcga taaccacgcg ttgttcggaa
     6901 tcagaggctt tgatgatacc gattttccac gcgttttcac cgttggcatt gagcagggcg
     6961 agggctttgt ccacttccgg agcaggcagg gcaataatca taccgacgcc gcagttgaag
     7021 gtgcgataca tttcatggcg ctcaacgtta cctgccgttt gcagccagtt gaacacttcc
     7081 ggccactgcc aggaagattc atcaatcact gcctgggtat tatctggcaa tacgcgcgga
     7141 atgttttccc agaagccgcc gccggtcagg tgcgcaatgg catgcacatc gaccttttca
     7201 atcaatttca gcactgactt cacgtaaatg cgggtcggtg ccagcagatg atcggctaat
     7261 ggcttaccat caagttcggt ggtttgcgga tcacaaccgc tgacttcaag aattttgcgc
     7321 accagcgaat agccgttcga gtgcgggccg ctggagccga gtgcaatcag cacatcgccg
     7381 tcgctgactt tagatccgtc gatgatttct gatttttcta ccacgccaac gcagaaacca
     7441 gcgacatcgt aatcttcacc gtgatacatc cccggcattt ccgccgtttc gccacccacc
     7501 agtgaacagc ccgattgcag acaaccttcc gcaatgccgc tgatcaccgc tgaagcggta
     7561 tcaacatcca gttttccggt tgcgtaatag tcgaggaaaa acagcggttc cgcaccttgc
     7621 accaccaggt cattaacgca catggcgacc agatcaatac caatggtgtc gtgacgtttt
     7681 aagtccatcg ccagacgcag cttggtacct acgccgtcag tgccggaaac cagcacaggt
     7741 tcacgatatt tttgcggcaa tgcacacagc gcaccgaagc cgcccagacc gcccatcact
     7801 tccggacgac gcgttttctt cactacgcct ttgattcttc caaccagagc attacccgcg
     7861 tcaatatcaa caccggcatc tttgtagcta agagaggttt tatcggtcac tgcttgggtc
     7921 cccacgcgtt acttgcggta gaaaaataaa attcggcgca attctaacag ggaaagcaaa
     7981 cgtttgcgag actgctttac acaacctttt tgcacgtctt ttccccaggc gcgcggcgaa
     8041 agaatacttg taccagggca aaggatcgtt ttcggatgga ataatcttct
//
LOCUS       DAJOTB010000194         8068 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJOTB010000194 DAJOTB010000000
VERSION     DAJOTB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.194"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            2..1723
                     /gene="iucA"
                     /locus_tag="OHN28_004354"
     CDS             2..1723
                     /gene="iucA"
                     /locus_tag="OHN28_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCQ2299630.1"
                     /translation="MLPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQ
                     KSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCE
                     TFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFNR
                     QEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQ
                     WLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKF
                     SLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLN
                     GNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQ
                     QAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAF
                     MPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLMA
                     RVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFAN
                     PLQAQEV"
     gene            1724..2671
                     /gene="iucB"
                     /locus_tag="OHN28_004355"
     CDS             1724..2671
                     /gene="iucB"
                     /locus_tag="OHN28_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCQ2299631.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2671..4413
                     /gene="iucC"
                     /locus_tag="OHN28_004356"
     CDS             2671..4413
                     /gene="iucC"
                     /locus_tag="OHN28_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCQ2299632.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4410..5687
                     /gene="iucD"
                     /locus_tag="OHN28_004357"
     CDS             4410..5687
                     /gene="iucD"
                     /locus_tag="OHN28_004357"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCQ2299633.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            5769..7970
                     /gene="iutA"
                     /locus_tag="OHN28_004358"
     CDS             5769..7970
                     /gene="iutA"
                     /locus_tag="OHN28_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCQ2299634.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1870 a         2135 c         2163 g         1900 t
ORIGIN      
        1 gatcctgccc tctgaaaaat ccgccacaga tgtggctgcg cagtgcttcc tgaatgcctt
       61 gattcgtgaa accaaagact ggcaactggc ggaatacccg ccagacgaat tgattatccc
      121 gctggacgaa cagaagtcgc tccatttcag agtggcttat ttctccccaa cccagcatca
      181 ccgctttgca tttcctgctc atctggtcac ggcatcaggc agttatcctg tcgactttac
      241 cactctctcc cggcttatta ttgataagct ccggcatcaa ctttttctgc cagttcccct
      301 ctgcgaaact ttccaccaac gcgtgctgga aagctacgcc catacgcaac agacaattga
      361 tgcccgccat gactgggcca tcctgcgtga aaaagcgttg aattttggcg aggctgagca
      421 ggcactgctg acaggacacg ctttccaccc tgcacctaag tctcatgaac cgtttaaccg
      481 ccaggaagct gaacgatacc tgcctgacat ggcacctcac ttcccgctgc ggtggttttc
      541 ggtggataaa acgcaaatcg ctggtgaaag tctgcatctt aaccttcaac agcggttgac
      601 gcgatttgcc gcagaaaatg cgccccagtt actcaacgaa ttaagtgaca atcaatggct
      661 gttcccgctg cacccgtggc agggagaata tcttttgcag caagtgtggt gccaggcact
      721 ttttgctaaa ggacttatca gagacttagg cgaggccggc acgtcgtggc tgccgaccac
      781 ctcttcccgc tccctctact gtgctaccag ccgcgatatg atcaagttct ccctgagcgt
      841 tcggctgacc aactccgtcc gtactctgtc tgtgaaagag gtggagcgag gaatgcgcct
      901 ggcacgtctg gcgcaaaccg acggctggca gatgctacag gcccgcttcc ctactttccg
      961 ggtaatgcag gaggacggct gggccgggct gcgcgatctt aacggcaaca tcatgcagga
     1021 aagtttgttt tccctgcgtg aaaacctgct gctggagcag ccgcaaagcc agaccaacgt
     1081 tctggtttcc ctgactcagg ccgcaccgga tggcggtgat tcgctgctgg tttcggcggt
     1141 aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccacg catgggtcga
     1201 tgcttactgt cagcaggtgc tgaagcctct gtttacggct gaagcggatt acggcctggt
     1261 gctgctggcg catcagcaaa atattcttgt gcagatgctt ggggatctgc cggtcggatt
     1321 tatttaccgt gactgccagg gcagcgcgtt tatgcctcac gcgacagagt ggctcgatac
     1381 cattgacgag gcgcaggcgg aaaatatttt cacccgtgag cagttgctgc gctatttccc
     1441 ttattacctg ctggttaact ccacttttgc cgtaactgct gcgctgggtg ctgccgggct
     1501 ggacagcgaa gcgaatctga tggctcgtgt acgaacattg ctggctgaag tgcgtgacca
     1561 ggtgactcat aaaacctgtc tcaactatgt tctggaaagt ccgtactgga acgtaaaagg
     1621 taactttttc tgttatctga acgatcataa cgagaacacc atcgttgacc cttcggtgat
     1681 ctattttgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg gggcaaacat
     1741 tgttcacagc ggatatggac tgcgctgtga aaaactcgac aagcctctga atcttggctg
     1801 ggggctggac aatagcgcgg tgttgcactg gcccggggag ctgccaacag ggtggctgtg
     1861 cgacgcgctg gatcagatat ttatcgccgc accacaactt tcagcagtgg ttcttccctg
     1921 gtccgaatgg tgtgaggagc cacaggcgct gacgcttttc ggacaggtac aaagcgacat
     1981 tatccatcgt tccgctttct ggcagttacc gttatggctg agttctccgg caaaccgggc
     2041 ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc gccccccccg
     2101 tccgcagggt gaagtttatc gtcgttacga tccacgcatt cgcaggatgc tgagtttccg
     2161 cattgccgat cccgtttctg atgcagaacg tttcactcgc tggatgaacg atccgcgcgt
     2221 tgagtatttc tgggagcaaa gtggctcact ggaggtacag atcgcctatc tggaacgcca
     2281 gctcaccagt aaacatgcat tcccgctgat tggttgtttc gacgatcggc cgttcagtta
     2341 tttcgaaatc tactgggcgg cggaagaccg cattggtcgc cactattcgt ggcagccctt
     2401 tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggagccc actatgtgca
     2461 aagctggctg cgcggggtga cacattacct gctgctgaat gagccacgta cgcagcgcac
     2521 agtactggag ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata
     2581 ccggacaatt aaagagttcg acttcccaca taaacgctcg cgcatggtga tggcggatcg
     2641 tcatcacttc ttcacggagg tcggtctgta atgaatcaca aagactggga tttggtcaac
     2701 cgccgcctgg tggcaaaaat gttgtctgag ctggagtatg agcaggtttt ccacgcggaa
     2761 tctcagggcg atgaccgcta ctgcattaac ctgccgggag cacaatggcg cttcatcgct
     2821 gaacgtggta tctggggctg gctctggatt gatgctcaaa ctctgcgctg cgcggacgag
     2881 ccagtactgg ctcagacgct gctgatgcag ctaaagcagg tactgtcaat gagcgatgca
     2941 accgttgctg agcatatgca ggatttgtat gccacgctgc tgggcgacct gcaactactg
     3001 aaagcccgtc gcgggctgag cgccagtgac ctgattaatc ttaatgccga ccgcctgcaa
     3061 tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggtaaa
     3121 gaggcgctgg aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg
     3181 gtaaaacgtg aacatatgat ctggcgctgt gataacgaga tggatattca tcagttgttg
     3241 acggccgcaa tggatccgca ggagtttgcc cgcttcagtc aggtctggca ggaaaacgga
     3301 ctggatcata actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgct
     3361 accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac
     3421 cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg gggcgggctg
     3481 gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga
     3541 tacatcgctg ccggaccact ggcttcacgc tggctacaac aggtttttgc gaccgacgcc
     3601 accctagtgc aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta tgtctcccat
     3661 gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc
     3721 tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca
     3781 ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatataga ccgctccggg
     3841 ctggacgctg aaacctggct tacgcaactg ttccgggtgg tcgtggttcc tctgtatcac
     3901 ctgctttgcc gctacggtgt cgcgcttatt gcacatggac aaaatataac tctcgccatg
     3961 aaagaggggg ttccacagcg tgttctgctg aaagacttcc agggcgatat gcgactggtg
     4021 aaagaagagt tccccgaaat ggactctttg cctcaggagg ttcgtgatgt tacatcccgc
     4081 ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt
     4141 tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg
     4201 gcagcagtgt tgagtgatta catgaaaaaa catccacaaa tgtcagagcg ttttgcgctt
     4261 ttctcacttt tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg
     4321 cctgatctgg atggcggcag ccgcatgctg ccgaattacc ttgaggatct gcaaaatccg
     4381 ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtag
     4441 ggacagggcc atttaatctc agcattgctg cgttgtcaca tcagatcgaa gaactggact
     4501 gtctcttctt tgatgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt
     4561 gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct acaaatccct
     4621 acagttttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac
     4681 tacgtacagt atcccgtgaa gagttttctg actacctccg ctgggctgct gaagatatga
     4741 ataacctgta tttcagtcat accgttgaaa acattgattt cgataaaaaa cgtcgattgt
     4801 ttctggtgca aaccagccag ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa
     4861 aacaacctta tttaccaccc tgtgtgaagc atatgacaca atcctgtttc catgccagtg
     4921 aaatgaatct tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt ggtggaggac
     4981 agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa
     5041 taaactgggt ctcccggcgt aataatttta acgcactgga tgaggctgct tttgctgatg
     5101 agtattttac acctgaatat atttcaggct tctccggact ggaggaagat attcgccatc
     5161 agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca
     5221 tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc
     5281 tacccagccg ctcggtaaca actctggaaa gtagtggtcc tggctggaag ttgctgatgg
     5341 agcatcatct ggatcagggc agggagagcc tggaaagtga tgtggtgatt ttcgccacag
     5401 gttaccgttc tgcgttgcca caaatacttc cctcactgat gcccctgatc accatgcacg
     5461 ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaga
     5521 acaacatctt cgtggtcaac gccagtatgc aaacccatgg catcgccgaa ccccagctca
     5581 gcctgatggc atggagatct gcacgtattc ttaatcgcgt aatgggacgt gatttattcg
     5641 atctcagtat gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga aaacgcagcc
     5701 ggaggctgct tctttaactc gctacacagc atctttgggc tgattttttc cgcccgtatg
     5761 gaggaataat gatgataagc aaaaagtata cgctttgggc tctcaaccca ctgcttctta
     5821 ccatgatggc gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg gtgtctgcca
     5881 accgcagcaa tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc gaaaacgccg
     5941 aactggaaca gcagattcag ggcggcaaag agcttaaaga cgcactggct cagctgatcc
     6001 ctggccttga cgtcagcagc cggagccgca ccaactacgg tatgaatgtg cgtggccgcc
     6061 cgctggtcgt gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac agccgacaac
     6121 tggactctat agatcctttt aatatcgacc atattgaagt gatctccggt gcgacgtccc
     6181 tgtacggcgg cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa ggccagccgg
     6241 aaaccatgat ggagtttgag gctggcacca aaagtggctt tagcagcagt aaagatcacg
     6301 atgaacgcat tgccggagct gtctccggcg gaaatgagca tatctccgga cgtctttccg
     6361 tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc ttgcttgata
     6421 acacccagac cggcctgcag tactccgatc ggctggacat catgggaact ggtacgctga
     6481 acatcgatga atcccggcag cttcagttga tcacacagta ctataaaagc cagggcgacg
     6541 acgattacgg gcttaatctc gggaaaggct tctctgccat cagagggacc agcacgccat
     6601 tcgtcagtaa cgggctgaat tccgaccgta ttcccggcac tgagcggcat ttgatcagcc
     6661 tgcagtactc tgacagcgct tttctgggac aggagctggt cggtcaggtt tactaccgcg
     6721 atgagtcgtt gcgattctac ccgttcccga cggtaaatgc gaacaaacag gtgacggctt
     6781 tctcttcgtc acagcaggac accgaccagt acggcatgaa actgactctg aacagcaaac
     6841 cgatggacgg ctggcaaatc acctgggggc tggatgctga tcatgagcgc tttacctcca
     6901 accagatgtt cttcgacctg gctcaggcaa gcgcttccgg agggctgaac aacaagaaga
     6961 tttacaccac cgggcgctat ccgtcgtatg acatcaccaa cctggcggcc ttcctgcaat
     7021 caggctatga catcaataat ctctttaccc tcaacggtgg cgtacgctat cagtacactg
     7081 aaaacaagat tgatgatttc atcggctacg cgcagcaacg gcagattgcc gccgggaagg
     7141 ctacatccgc cgacgccatt cctggcggct cagtcgatta cgacaacttc ctgttcaacg
     7201 ccggtctgct gatgcacatc accgaacgcc agcaggcatg gctcaacttc tcccagggcg
     7261 tggagctgcc ggacccgggt aaatactatg gtcgcggcat ctatggtgct gcagtgaacg
     7321 gccatcttcc tctaacaaag agtgtgaacg tcagcgacag caagctggaa ggcgtgaaag
     7381 tcgattctta tgagctgggc tggcgcttta ctggcaataa tctgcgtacc caaatcgcgg
     7441 cctactattc gatttctgat aagagcgtgg tggcgaataa agatctgacc atcagcgtgg
     7501 tggacgacaa acgccgtatt tacggcgtgg aaggtgcggt ggactacctg attcctgata
     7561 ctgactggag taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa gtgaacggta
     7621 cctggcagaa atacgatgtg aagacagcaa gcccatcaaa agcgacagcc tacattggct
     7681 gggcaccgga cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac gtgagcgacg
     7741 cgcagggcta caaggtcgat ggctatacca ccgtggatct gctcggcagt tatcagcttc
     7801 cggtgggtac actcagcttc agcattgaaa accttttcga ccgtgactac accactgtct
     7861 gggggcagcg tgcaccactg tactacagcc cgggttacgg cccagcgtca ctgtacgact
     7921 acaaaggcag gggccgaacc tttggtctga actactctgt gctgttctga ccggtattcc
     7981 tttacaacaa aggtacgctg atatcaacat ggccgctgac agccaagttg atatcatata
     8041 atacacgaca taatctgtag tcagggag
//
LOCUS       DAJOTB010000195         8004 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJOTB010000195 DAJOTB010000000
VERSION     DAJOTB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.195"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            208..384
                     /locus_tag="OHN28_004359"
     CDS             208..384
                     /locus_tag="OHN28_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299635.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(421..2340)
                     /gene="dhaR"
                     /locus_tag="OHN28_004360"
     CDS             complement(421..2340)
                     /gene="dhaR"
                     /locus_tag="OHN28_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCQ2299636.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            2568..3638
                     /gene="dhaK"
                     /locus_tag="OHN28_004361"
     CDS             2568..3638
                     /gene="dhaK"
                     /locus_tag="OHN28_004361"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCQ2299637.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3649..4281
                     /gene="dhaL"
                     /locus_tag="OHN28_004362"
     CDS             3649..4281
                     /gene="dhaL"
                     /locus_tag="OHN28_004362"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCQ2299638.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4292..5710
                     /gene="dhaM"
                     /locus_tag="OHN28_004363"
     CDS             4292..5710
                     /gene="dhaM"
                     /locus_tag="OHN28_004363"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCQ2299639.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6030..7727
                     /gene="treA"
                     /locus_tag="OHN28_004364"
     CDS             6030..7727
                     /gene="treA"
                     /locus_tag="OHN28_004364"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2299640.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         2070 a         2057 c         1922 g         1955 t
ORIGIN      
        1 acaattcaac gtctaattcc gctaatacta ctctggcggt taataacgag ccattatgtg
       61 agttgcacgt aatggctttt ttacgcctgc tacgcgtctt ctttcaattc tgagttacca
      121 aaactcataa tcagactcct gacaatatta acaatgcagc ttttatgtga taacaatcac
      181 agtgcattaa ccgttcaaga gtttgtcatg aaaaaaacat ttaccttcag cctgttagca
      241 attgtctttg caagcctgtt gagtgcttgt gttccccacc atcatcaccg ccacagcgat
      301 ggccctcgtg gtcctgcgcc ctccggtaag ccaatgccac cttcaaatgg tggaccaaat
      361 cacggcagtc atggtcgcta ttaattagta attcatagcg ggaaagtaca tactctcccg
      421 ctatccacgg cgcttaaact gccctgcatc aatgccatgt tgcttcattt tccgccacag
      481 cgtagtgcgg ccgattccaa gtaaagccga catttcctga atgcgaccgc ctgtgacttg
      541 ggctgcgtta ataattgcct ctttttcaac ttccgcaaat gacagactgg tggaaaggcg
      601 ggtggcgctg acatcatctg tcgcctgctc ggtaaacaga tgttccggca aatcactgac
      661 gcgaatgcgc ccattatcac tactcagagc aagattctcg atgacgctgt aaagttcaaa
      721 atcgttgcca ggccatgcac aggaaaccag gcgagcgagg gcatcgtcat caattttcag
      781 ccgcgtagag aagcgttttt caaggctgcg taatttgtta ttcaccagcg ccggaatgct
      841 gccacgccgc atacgcagtg gcgggatggt aatttcaaat gcatgcagcg cgtaatacag
      901 ctggcgacta aaacgatttt gttccaccag cattgcgagg tccgcggtcg ttgtggcaat
      961 cactttgaca tcaatgggta ttaaacgccg cgcatccagt cgcgtgataa caccctgctt
     1021 gataacctga agcaaagcag actgtaactc caccgccaga tattcaatct tttcaagaaa
     1081 cagcgtgccg ccgtgcgcca gttccagccg actcagacgg ccattttcat tatccgtgcg
     1141 atcgccacca ataaattctt ccgccagcgc cgcatcacca tataactcac aattgacggc
     1201 gatataaggc cccgctgcac gctcgctttc attatgaatt gcctgactta gcagtgcctt
     1261 gcccacgccc tcttctccac aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc
     1321 aaaatgaatc aagcggcggg tttgcggatc gtcctgtggc atatgagcga aggtatggct
     1381 gacttttcct aattgactgg tcattaactg ccgcatctgt tccacaggat ggagcaataa
     1441 aataaagctg gttccctgcg tttctattat cggtttaagg gttatcaccg catcaataaa
     1501 ctgatgctga ctttcaaagg ttgcttctac gtgtttgagc ggatgtgcct gttttattgc
     1561 ttgttgcaat acggcgggta acgttaagag ttcagtgatt gcccttccct gacttgccgt
     1621 cgcgtcaagg cgcaagactc gcgccgcctg ggcgttgata aattgcaaat taccctgctc
     1681 gtcccagcta atcacgccat catccatact ttctaacagg gcattaagtt gatttaaatg
     1741 acggttagtt tcagcgagca aactgtccgt cagcagtaaa tttccgacct cgcgtgcgat
     1801 tgccaacgtc aacggcaaat cagctgcggt agtttgctca accggacacg ccagcgctat
     1861 tgttcccgtc aatcggccct tgctgtcaaa caacggcgtt gcgcaaaagg cccagttcca
     1921 gagtgcctgt ttgaaatgtt gatcggccat cgttttcacg gcctgaccag agatagccgc
     1981 taacgatagc gcacaagtac caataattcc ctcggcgcaa tacgtgccgt cattgaatcc
     2041 cagtacactt agctgctgca aggtttgcgg atcgccatta cggctgagaa tgcaggcggt
     2101 ttcatcgagg ataagcaacg cacactctcg cggtgccata tattcccagg catcttccag
     2161 cgcagcctgc ccgagcgtca gcatcgcttt cttacgccga taaatagaag caaatgtcac
     2221 gccctgggcc tggtgtggta cgttccatgt ctcccgtttc atcagcttat tgcatctctc
     2281 ccaggaggtt gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag cgccactcat
     2341 atcccgcatc ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat taatacacgt
     2401 aaaagtttca atatggaaca ctcattaaag catatgtttc actatgaaac ataatttggg
     2461 ctaattttct tttcccttgc ctgatgcaca atggattcat cggcagtcag tggtcgccgt
     2521 gtcgttgaac atcatccatg ccctaccgta attgctggag caaaataatg aaaaaattga
     2581 tcaatgatgt gcaagacgta ctggacgaac aactggcagg actagcgaaa gcgcatccat
     2641 cgctgacact gcatcaggat ccggtgtatg tcacccgagc tgatgccccc gttgcaggaa
     2701 aagtcgccct gctgtcgggt ggcggcagcg gacacgagcc gatgcattgt ggttatatcg
     2761 gtcaggggat gctttcgggg gcctgtccgg gcgaaatttt cacctcaccg acgcccgata
     2821 aaatctttga atgcgccatg caaattgatg gcggcgaagg tgtactgttg attatcaaaa
     2881 attacaccgg cgatattctt aacttcgaaa cagcaaccga gttactgcac gatagcggcg
     2941 taaaagtgac cactgtggtc attgatgacg acgttgcagt aaaagacagt ctttataccg
     3001 ccgggcgacg cggcgttgcc aacaccgtat taattgaaaa actcgtaggt gcagcggcgg
     3061 agcgtggcga ctcactggac gcctgtgcgg aactggggcg taagttgaat aatcaaggcc
     3121 actcaatagg tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta
     3181 ccctggcgga taatgagatg gagtttggcg tcggcattca tggtgagcca ggtattgacc
     3241 gccgcccctt ctcttccctt gatcaaaccg tcgatgaaat gttcgacacc ctgctggaaa
     3301 atggctcata ccatcgcact ttgcgtttat gggattatca acaaggcagc tggcaggaag
     3361 aaccacaaac caaacaaccg ctccagtctg gcgatcgggt gattgcgctg gttaacaatc
     3421 ttggcgcaac tccgctttct gagctgtacg gcgtctataa ccgcctgacc acacgttgcc
     3481 agcaagcggg attgactatc gaacgtaatt taattggcgc gtactgcacc tcactggata
     3541 tgaccggttt ctcaatcacc ttactgaaag ttgatgacga aacgctggca ctctgggacg
     3601 ccccggtcca caccccggcc cttaactggg gtaaataagg agaaagcaat gtcactgagc
     3661 agaactcaaa ttgttaactg gctcactcgt tgtggcgata ttttcagcac cgagagcgag
     3721 tatcttactg gactggatcg cgagattggc gacgctgacc acgggctaaa tatgaaccga
     3781 ggctttggca aagtggtgga aaaactccct gctatcgcag ataaagatat cggtttcatt
     3841 ctcaagaata ccggcatgac gctactttcc agcgtcggag gtgccagtgg tccgctgttc
     3901 ggtaccttct ttatccgcgc cgcacagaca acccaggcac ggcaaagcct gacgctggaa
     3961 gagctttatc agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa
     4021 cctggcgata aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc
     4081 tgcgagcaaa atctctctgt tccggcggcg ctcgacgctg ccagtagcat cgccgaatcc
     4141 gctgcacaaa gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa
     4201 cgcagtattg gtcaccagga tcccggcgcg acctcggtga tgtttatgat gcaaatgttg
     4261 gcgttagccg caaaagagta aggaattggt gatggtaaac ctggtcatag tttcacatag
     4321 cagccgactg ggagaaggtg tcggtgaatt agcccgtcag atgttaatga gtgataattg
     4381 taaaatcgcc attgccgcgg gaattgacga tccacaaaat cccattggta ccgatgccgt
     4441 caaagtgatg gaggccatcg aatctgttgc cgatgccgac catgtgctgg tcatgatgga
     4501 tatgggtagc gcattattga gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc
     4561 cgcaaaagta cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag caacggtcag
     4621 cgcggcctcg ggggcggata tcgacaaagt tatctttgac gccatgcatg cgctggaagc
     4681 caaacgtgaa caactgggtt taccgtcctc cgacactgaa atctctgaca catgtcctcc
     4741 gtacgatgaa gaagcccgtt ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt
     4801 acgtccggcc tcccggctgg tttatacctt atcgacattt aatgccgata tgttgctgga
     4861 gaaaaacggc aaatgcgtta caccggagag tattaaccag attgcgttac tacaagttcg
     4921 ctataacgat acgctgcgcc tgattgcgaa aggaccagaa gctgaagagg cactgatcgc
     4981 tttccgtcag ttggctgaag ataactttgg tgaaacggag gaagttgctc cacctactct
     5041 gcgtcccgtt ccgcctgttt cgggtaaagc cttttattat caacctgttt tatgtacggt
     5101 acaggcaaaa tcaaccctga ccgtggaaga agaacaagaa cgattacgcc aggccattga
     5161 cttcacgtta ttagatctga tgacgttaac agcgaaagca gaaaccagcg ggcttgacga
     5221 tattgccgca atcttttctg gtcaccatac actgttagat gatccggaac tgcaggcggc
     5281 ggcaagcgaa ctccttcagc atgaacattg cacggcggaa tatgcctggc agcaagttct
     5341 taaagaactt agccagcaat accaacagct ggatgatgaa tatctacagg ctcgctatat
     5401 cgatgtggac gatcttctgc atcgcaccct ggtccacctg acccaaacga aagaagaact
     5461 cccgcagttt aactcgccaa ctattctact ggcggagaac atttatccct ccacagtact
     5521 gcaactggat ccagcggttg taaaaggtat ctgccttagc gccggaactc cgctatccca
     5581 cagtgcccta atcgcccgtg aactgggaat tggctggatt tgccagcagg gtgagaaact
     5641 gtatgcgata caaccagaag aaacgctaac gctggacgtt aaaacgcaac gtttcaaccg
     5701 tcagggttaa accaccgttc ggatggcatc attctgatgt catccggcaa tttactctgc
     5761 actgatgttt tttaactacc tgttatattt ttcaccaaat tatgatgatt tttcttcgct
     5821 aatcgcgaac ccaacgtcgc tggctgacga ataaacagat gttattttat cactctattt
     5881 cgccgttatt tatcccttta attccctttt ctaaaatgtc tgtcagttcg cagaatgtga
     5941 tttcgatcat gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg aataccgttt
     6001 tattcggttt cgccaaagga gaatgattga tgaaatcccc cgcaccttct cgcccgcaaa
     6061 aaatggcgtt aattccagcc tgcatctttt tgtgtttcgc tgcgctatcg gtgcaggcag
     6121 aagaaacatc ggtaacacca caaccgcctg atattttatt agggccgctg tttaatgatg
     6181 tgcaaaacgc caaacttttt ccggaccaaa aaacctttgc cgatgccgtg ccgaacagcg
     6241 atccgctgat gatccttgct gattatcgga tgcagcaaaa ccagagcgga tttgatctac
     6301 gccatttcgt taacgtcaat ttcaccctgc cgaaagaggg cgagaaatat gttccgccag
     6361 aggggcaatc actgcgcgaa catattgacg gactttggcc ggtattaacg cgttctaccg
     6421 aaaacaccga aaaatgggat tctctattac cgctgccaaa gccttatgtc gtgccaggcg
     6481 gacgttttcg cgaggtatat tactgggaca gttacttcac catgttagga cttgccgaaa
     6541 gcggtcactg ggataaagtc gcggatatgg tggccaattt tgctcatgaa atagacactt
     6601 acggtcatat tcccaacggc aaccgcagtt actatttaag ccgctcgcaa ccgcccttct
     6661 ttgccctgat ggtagagtta ctggcgcagc atgaaggcga cgccgcgttg aaacaatacc
     6721 tgccgcaaat gcaaaaagaa tatgcttact ggatggacgg tgttgaaaac ctgcaagccg
     6781 gacaacagga aaaacgcgtt gtaaaacttc aggatggtac ccttctcaac cgctactggg
     6841 acgatcgcga tacgccacga ccagagtcat gggtggaaga tattgccact gccaaaagca
     6901 atccgaatcg acctgccact gaaatttacc gcgacctgcg ctctgccgct gcgtctggct
     6961 gggatttcag ctcgcgctgg atggacaatc cgcagcagtt aaacacctta cgcaccacca
     7021 gcatcgtacc ggtcgatctg aacagcttga tgttcaaaat ggaaaaaatc ctcgcccgcg
     7081 ccagcaaagc tatcggagat aacgcgatgg caaaccagta cgaaacgctg gcaaatgccc
     7141 gtcaaaaagg gatcgaaaaa tacctgtgga acgatcaaca aagctggtat gccgattacg
     7201 acctgaaaag tcataaagtg cgcaatcagt taaccgcggc cgccctgttc ccgctgtacg
     7261 tcaatgcggc agcgaaagat cgcgccagca aaatggcgac ggcgacgaaa acacatctgc
     7321 tgcaaccagg cggcctgaac accacgtcgg tgaaaagtgg gcaacaatgg gatgcgccaa
     7381 acggctgggc gccgttgcag tgggtcgcga cagaaggatt acaaaactac gggcaaaaag
     7441 aggtagcgat ggacattagc tggcacttcc tgaccaatgt tcagcacacc tatgaccggg
     7501 agaaaaagct ggtggaaaaa tatgatgtca gcaccaccgg aacagggggc ggcggtggcg
     7561 aatatccatt acaggatggc tttggctgga ccaatggcgt gacgctgaaa atgctggatt
     7621 tgatctgccc gaaagagcaa ccgtgtgaca atgttccggc gacgcgtcca ctcagtgaat
     7681 caacaacaca gccgctaaaa caaaaagagg cggaacctac gccttaactt tactgtatat
     7741 cgagcaatgc cattacgtgc ataaaattgg cattgctctt tcttcatcac gaaataccac
     7801 tctttctacg gtctatgcct ttattttctt ttattagaga agattttaat tttattttat
     7861 caacttgtta atcatcacac ctctatttat attttccccc actccccatt ctccctgaca
     7921 attttcttgt ggaacaaagt gttatttaag aaacaagcaa atcattctgt ctctttagac
     7981 aaattttgac atcaaacatg atct
//
LOCUS       DAJOTB010000196         7977 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJOTB010000196 DAJOTB010000000
VERSION     DAJOTB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.196"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(40..453)
                     /locus_tag="OHN28_004365"
     CDS             complement(40..453)
                     /locus_tag="OHN28_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2299641.1"
                     /translation="MNTKTKNMAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCN
                     GVTPRKDKLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRG
                     LPESEKNKLINELKEKREHFDLLLKELLEAKNQTK"
     gene            552..782
                     /locus_tag="OHN28_004366"
     CDS             552..782
                     /locus_tag="OHN28_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2299642.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTEAA"
     gene            841..1317
                     /locus_tag="OHN28_004367"
     CDS             841..1317
                     /locus_tag="OHN28_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299643.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPELTIQEIDEAIAALVATRGAVIRTAQDHHA"
     gene            1357..1581
                     /locus_tag="OHN28_004368"
     CDS             1357..1581
                     /locus_tag="OHN28_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299644.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            1578..2333
                     /locus_tag="OHN28_004369"
     CDS             1578..2333
                     /locus_tag="OHN28_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCQ2299645.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGVLLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEIITDGESADADTQQPADQRINYQV
                     ILDTYHEILPEMPPVKILTDGRKKHLRTFWKKFNFNESRWRAYLEFIGGNCRWMLEDR
                     PNGRGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            2323..3738
                     /locus_tag="OHN28_004370"
     CDS             2323..3738
                     /locus_tag="OHN28_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2299646.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIKGLFQADKPTDLITLTELMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMIQPADMTATDRITAVQQVIGQVAEHSRTGHKGG
                     LRPASEVVDDWIDDLERRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPNLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRLT
                     NDRSEKNKRYSRSDNSRTNEF"
     gene            3777..4187
                     /locus_tag="OHN28_004371"
     CDS             3777..4187
                     /locus_tag="OHN28_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299647.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAKPARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            4189..4425
                     /locus_tag="OHN28_004372"
     CDS             4189..4425
                     /locus_tag="OHN28_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299648.1"
                     /translation="MAGQSDYLPPGLPHNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYREFFRERLNYWRGVMEQ"
     gene            4422..4733
                     /locus_tag="OHN28_004373"
     CDS             4422..4733
                     /locus_tag="OHN28_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2299649.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSVKYLQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            4730..4954
                     /locus_tag="OHN28_004374"
     CDS             4730..4954
                     /locus_tag="OHN28_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299650.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            5636..6424
                     /locus_tag="OHN28_004375"
     CDS             5636..6424
                     /locus_tag="OHN28_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCQ2299651.1"
                     /translation="MTVQISAESLSPLHHNQLPVITTELLASLYGTEVNGIQQNFKRN
                     IARFVVGKHFFKLEGNELRDFKNRLTDSQSVAKHTRSLILWTERGAARHAKMLETEQA
                     WEVFEKLEDCYFSQRPERRSTTTDDRTPLRDAVNILVGKKGMMYPDAYTMIHQRFAVS
                     HIEQLSQTQMLEAIEYIHCLLLDENHPTIPDFSFITTIKNGKVTGMRHVAKGEHLMTF
                     DAFKEIAERAGYLVIHSDNLRSMTLDKLMVMGKKSAISVANSAF"
     gene            complement(6599..7522)
                     /locus_tag="OHN28_004376"
     CDS             complement(6599..7522)
                     /locus_tag="OHN28_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCQ2299652.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDEIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLSANE
                     YVQLSDAYRKNFNRNLDKQMESLFLENNANAFSKRITYTVQATDDQKNVFVKTSTDNP
                     ISVGAALLTSLSIITPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQAKYEAEKQIER
                     QAQLQPQKADNLDQSRMAFANADSDLSTVWSKLTPAKKKDLLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
BASE COUNT         2166 a         1764 c         2054 g         1993 t
ORIGIN      
        1 tgcatgccag cagcggctgg cctggaaatt tgaactgaac tattttgtct ggtttttggc
       61 ttcaagtagt tcttttagca gtagatcgaa gtgctctctt ttttctttta gctcattgat
      121 taatttattt ttttcgctct caggcagtcc tctgaatagt tgcagtagag cctgttcttg
      181 cggggttagt tcttgattgt aattacccgg gtgtagtgtg gattcatcca aatattcccc
      241 ttcattcatg aagaaaaaat gaacagggta tccggtcact tttgctagct tgtctaattt
      301 atcttttctt ggagttaccc cattacacca tgcctgaact gattgagccg taaccccaat
      361 atggcgagct aactcagatt gagaccagcc taagtctttt agaatccgtt gtagtcggct
      421 agcaaaagcc atgtttttcg tttttgtgtt cataccgtaa ggatacaagg tttaattgta
      481 gggagcattg caaatataat ttgtaattac agttattgtt tgtaatgatg acgcggacac
      541 aaggagtcca tatggataaa caactgcaag acaaaattct ttccgtaatg agtcaatccg
      601 agttagggcg gcgattaggg aaaaaaccac aaacggtaag cctctggttc aaaggacggg
      661 ttcccggtga agaggttttg cgcacatctg aagctcttga atggcgcgtg acccctcatg
      721 atcttagacc ggatctttac ccaaacccag atgacagctt gcctaaaacg gaagcagctt
      781 aactgttggt gagccccaaa tctgattaag cgtaatcaat ttttagcgac aggagacgct
      841 atggaaaacc aagaggaatt acaaaaagag attttgacct gggcggcaag ggcagggcag
      901 gaactcgtca cgattgaaat ctgccgggcc tggttcagtc agggacgcaa tgatgagttg
      961 agactacatg aatttgagga cgcggacggc aacgtggact ggagagccat caacaacaat
     1021 cggcagaaaa tctttcgctg gttacgtggc gagacaacgg cggcgcgccg aaaaactcag
     1081 gtgctggcca gtgtgatgaa agccgtgcta cccgcagaac ggcgggcacg tctggagtcg
     1141 ccgggcgatc ccgttttgct ggcaacgctg gcggcaaaag aaggggtgga agcgattaac
     1201 gctgtacatc tccacttcgc gccagagtta actattcagg aaattgacga agcaatagcg
     1261 gcgctggtag cgacgcgagg agcagtgata cgcactgcgc aagaccacca tgcatgagcg
     1321 cctgaccagc gtttaactta taccgaggaa agaccgatgt taagacatat tgaccgcatt
     1381 acctggcgta acggctggca cctgaatgga cgcccggcac atgttgcaga gatccgcccc
     1441 atattcgatg gccgcgtcgc agctgcacgt tctgtatggg aaaagtacga agaagaaaaa
     1501 gcgaaactgc gtgagcaaaa tctctctggc gctgcctatg aggctggctg tcgcgttctc
     1561 tcagaggctt tgggcatatg aacatcctcc cgttacttga tagacccata gccttccaga
     1621 gaagttttat ccgtcttgaa atgggcgtga ctgccgcatt gtttctgtcg cagttgacgt
     1681 actggacaaa cagaacaact gacgacggct gggtatataa aacccaggat gagtgggaag
     1741 aggaaacagg gctttcacga tacgagcagg agggcgcgcg caaaaaacta cgtggtctcg
     1801 gcgtcttgct ggaacgtaaa cagggcttgc ctgccaggct gtactacaaa atcgataatg
     1861 atgtgttgtg ccagctactt acgtccgcat acaaggatgc ggaaaaacca catacaggtg
     1921 aggggaaaac caccaggcct gtacgtggaa aaccaacaaa cattcttaca gagaatacta
     1981 cagagattat tactgatggt gaatcggctg acgccgacac ccaacaacct gccgaccaga
     2041 gaataaatta tcaggtgata ctcgatacct atcacgagat tttgccagag atgccaccag
     2101 tgaaaatcct cactgacggc agaaaaaaac accttcgcac tttctggaaa aaattcaatt
     2161 tcaatgagtc ccgttggcgg gcctatctgg aatttatcgg tggcaattgt cgctggatgc
     2221 tggaggacag gccaaatggc aggggaggat tctggcggcg taaaaatctt gattatctga
     2281 ttaccgaacg ctgctatgtt tcggtcaagg aagaacgcgc aaatgataaa taacgcgatt
     2341 gcactgtaca gcgttgacgc agaacaggct gttctgggct gtctgatgct gaataccgac
     2401 cacgacagaa ccaatgcggt atttgcgtta ctgaaaccgg aaacgtttta tatcaatgcg
     2461 catcaggtta tctaccggga gatcaaagga ttatttcaag ctgataaacc aactgacctg
     2521 ataacactga ccgaactgat ggaatcgaag gggctgtatg agcgcgtggg gggattcgca
     2581 tacctggctg aaatgagtaa ggcggcgatt ccggcctcaa tggtcaacta cgcgaaaatt
     2641 gtacgtgaaa aagccattct tcgttatgcc gtggaaaagt tgaattcttg tctggagatt
     2701 atgatccagc ctgcagatat gactgctact gacaggatca ccgccgttca gcaagtgatt
     2761 gggcaggtgg ccgaacattc ccgtaccggg cataagggcg gtttgcgtcc ggcaagtgaa
     2821 gtcgtcgatg actggataga tgaccttgag cgccgtttca atgatccagc acatgcagcc
     2881 ggactaacgc tcggaatcga aagccttgat cgcttaatgg caccaaagca ggcgttaaga
     2941 ggttcattgg tcgttattgg tgcccggcca aaaatgggca agacagccgc gtttaataaa
     3001 attgcggtgc attttgctct gaatcatcgt ttgccgacgc tggtatttag cctggaaatg
     3061 actgacagaa gtctcattga acgcatggtt gcgcaggaag caaaggtcaa ttcggagatt
     3121 ttttatcttg gccctaacga tgaatctgaa ttgagtctcg caatagcaaa ggcgggggag
     3181 attgccgaat ccaacatgat gattgacagc acggcaggtg taacccttgc gcatattgtc
     3241 gctgaatgtc gaaaagttaa acggatgcgt ggctcggttg gcctggtggc aattgactat
     3301 ttgacgctga tgaagaccga atcggcagag cgtagggata ttgcgtatgg cgatatcaca
     3361 accggtttga agaacctggc aaaagagctg gattgtgttg tggtcctgct gacgcaactc
     3421 aaccgaaagc ttgaagaacg tgccgataag cggcctaacc cgagcgacag taaagatacc
     3481 gggcagatcg aacaagattg cgatgtctgg attggcctct atcgtgatgc tgtttataac
     3541 gaaaatgccg acccaaatct catggaaatg ttgctacgtc tgaaccgcga aggaccatcg
     3601 ggaacagcct atgccctgat gaaaaatggc tcagttattg atatcagcga tgaggaggtt
     3661 tatcgcctta ccaacgaccg ctcagaaaaa aataaacgtt attcccgtag cgataattct
     3721 cgcacaaatg aattttaacc acgcctgacc agcgtgaaat aaccgaggaa agaccaatga
     3781 ccaccactcc aggcaaatta gagtacccgt cggacaaagg acacatagac gacggtaaaa
     3841 actatctcga cgtcattttg tggaatatga acgtcgggcc acgtgcgcgc actcgcgcgg
     3901 tttttgtgcc aagacctaaa gcaggcaatt tttcaacccc tgctaagcct gcccgtcagg
     3961 catccgtagc cgcagcgcca gcagtgaaac gtaagggcaa aacgcacacc gggatcgcga
     4021 ttcgcagaaa tggtgagcgg caagtgaaac tccacgaaac agcgacaacc tggtgtgcct
     4081 caccgcatga aacttacgac aagataaccg ggcagcgcat tggcgcgcct ggtcgctgcc
     4141 gcctgctgct gagttccatc actccgatag caaagaaagg ggcgtgatat ggccgggcaa
     4201 tcagattacc tgccgcccgg cttaccacac aaccgcgcca aatggcccca ggaataccag
     4261 cttaaagagc actacgacat gcgagcggcg gcgctgatcc gccaactctt tgagaaacgt
     4321 attccgaggg gaagcgtgat tgagcaaatc ggaatgacgc cagatacgta ccgggaattt
     4381 ttcagagagc gtctgaacta ctggagaggg gtgatggaac aatgaaatac aaacggtggg
     4441 tgcgtgcgga agtcgtaatc atcaaacaat gtgcgggcag catgacagtt gaacgtatag
     4501 ggcagcttat tggcagaacc ggtgcagcgg tacgcacaaa agcgcgcgag ctgaaaatct
     4561 gtatgtatct gcggggtaat tatcaccagt cagtgaaata cctccaggaa gatatcgagc
     4621 tggcaaggga attacatcag tcgggtatta atcgccagga tatcgcagaa aaactcgaaa
     4681 tgccgatcgg agcggtgaat cagtttgttt attttgagcg gagaatttca tgaaagaact
     4741 ttttcttgca tttgtgcccc ggtttattaa tgaccagatc gcactgactg ataatggtga
     4801 acaatatgaa attgcctgca gcatggtgga tgtgaatcct ggcgaacggt atgacgcgat
     4861 gtgtgacctg aaaatattta cctggctggg ttgggctatt ccgtgtggag aaccaaccaa
     4921 tattcgtccg tttgagagca gggaggctgt atgagtgaat tgaaaatacg tcgcaaggca
     4981 atagcacgcg ttaaaaatct attaccagtg cctgtcaaat atcgtttttg tggtgggaat
     5041 gtacggattg gtacggatgt atggtgattg cggagtgacg taaaacgaat cgttgtgggt
     5101 gtaatatcgt caatatcatt ggtttcccgg agggaaagga catgtttggc gataaactgg
     5161 agttaccacg gaacgggcgg atagtgctct atatttcaga ttacaaagta acccgggctg
     5221 aggtgttgga accggaaaaa catctggtca cgttaccgga cattattgag ttgtataagc
     5281 gggtcggata tgtggacttg catcgcgatg atgttcccgt tgagtgtatg gaatgtgaac
     5341 accggaggtt tgaagttgat tgaattgact tagaaaaagt ccggggctat agtttccgcg
     5401 cagccgcaaa atcggttgcc gggattggcg tcccggatat ctaagtgacg catagccgcg
     5461 ttagcggttt ttttatgtgt taggcacggc cacattcgca ttatggtggg ctgtgtgggg
     5521 gcttctgtgg aagcgccggg ttcacttagc cggttacgcc aaccctgcac agctcaccac
     5581 cagatgattg gcgtcgctgg tggtgatgaa aaaaacacta agtgaggcca ctgcgatgac
     5641 agtccaaata tctgcggaat ccctttctcc gctacatcat aaccaacttc ccgtcatcac
     5701 tactgaattg cttgccagtt tgtatggcac ggaagtaaat ggtatccagc aaaacttcaa
     5761 gcgcaacatc gccagatttg tcgtaggaaa gcattttttt aagctggaag gtaatgaatt
     5821 acgcgacttc aaaaacagac tgacagatag tcagtcagtt gctaaacata cccgatccct
     5881 cattctctgg acagaacgcg gcgcagctcg ccatgccaaa atgctggaaa cagaacaggc
     5941 ctgggaagtt ttcgaaaagc tggaagattg ttattttagc cagcgtccag agaggcggag
     6001 tacaactacc gatgaccgta ctcccttgcg tgatgccgtt aatatactgg tcggcaagaa
     6061 aggcatgatg tatcccgatg cctacaccat gattcatcaa cgcttcgccg tctctcacat
     6121 agaacaactg tcacagacgc aaatgttaga ggcgattgaa tacatccact gcctgctact
     6181 cgatgaaaat cacccaacca ttccagattt ttcttttatc accactatca aaaatggaaa
     6241 agtgaccggg atgcgccatg ttgccaaagg cgaacatctg atgacctttg acgctttcaa
     6301 agagatcgct gagcgggcag ggtatctggt gatccacagc gacaatctgc gaagtatgac
     6361 actggataaa ttgatggtaa tggggaaaaa gtcggctata tccgttgcta attcagcatt
     6421 ttgacaggtt gtttggggcg gaaatactct atttgcgctt aaaaaggtat ttgaccacga
     6481 ccagcacgag accgaatacc tctgtcaaag taccgcccat ataaaacagt tacatatgca
     6541 cttgatgact aagctggcac caggttaaca cctggcgcca gcttgaagga gacttcagtt
     6601 attggtttct gaggaaagcg attcttgaaa gggtcatttg cgtctggcag tcgaccattt
     6661 tcttgacttc agcatctgtt ccctgcattg aaactttacc gcacatagca tccttagttt
     6721 tgatccactg acgctgagaa ggcagcaaat cttttttctt cgccggggtt aacttgctcc
     6781 agacggtact caaatcagag tcggcattcg caaatgccat tcgggactga tcaaggttgt
     6841 cagctttttg cggctgaagt tgtgcctgtc tctctatttg cttctccgcc tcatacttag
     6901 cctgttcttg cgccctgagt tgcacctgtt gttccgcttc aatttgactc tgttgggcat
     6961 ccttagcctg ctggattttc tgctgctcaa cgatcggggt gataatggaa agcgatgtca
     7021 gtaaagcggc gcccactgat atcggattat cagtagaggt ttttacaaaa acgtttttct
     7081 gatcgtcggt cgcctgcacg gtgtaggtga tacgttttga aaaggcgttt gcgttgttct
     7141 ccaaaaacag gctttccatt tgcttatcga gattacgatt aaaattcttt ctgtaagcat
     7201 ctgaaagctg gacgtactcg ttcgcagata gagtcatcgt cacggtccct tcacaagttt
     7261 tcattgtgct gcccgtgtca cttgatgttg tggagatctc agagatggct agtttgatct
     7321 tgtccagtgt gcttcgtttg gtctgattag tgacatcagg gtatttatcg acctgttcag
     7381 agagtccttc ataagcagat ttttttaata aatccatgag agctgactgg gttatttccg
     7441 aagaacaacc gatctcatct tttttgttat cacagcctgt aagggcaatg gcgagtaaga
     7501 gtactgagta ttttaatctc atgaattccc tttagagtaa ggattggttt ttaagacgcg
     7561 gtctgtaatc agtcccgaga gctacagtaa acaacgctga gcactgaacg catcacaggt
     7621 aaggtaaaag gtgatatcgg caaaggctgt gaaaccttta attcaacaga tgggaacggt
     7681 atgcgcagga tcctgtaacg acgctcccaa aaccggaaac ctgtaaggca tgttttcgct
     7741 gctgaactta atatacgaac ctgcactatc atgaacatat tttatatcgt tataccccga
     7801 gtgattgtgc ccggcaatgg atagtgtcct ggaggaacta tttctactgt aaaaggtaga
     7861 tatttaattt cagttttgaa ttattccctg ctgttaataa caatggctgt atatggcacc
     7921 gagtaggcat tttttcgctt actgaagggc agctgtgagc gaggaacaga ctttccc
//
LOCUS       DAJOTB010000197         7817 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJOTB010000197 DAJOTB010000000
VERSION     DAJOTB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.197"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            88..177
                     /gene="kdpF"
                     /locus_tag="OHN28_004377"
     CDS             88..177
                     /gene="kdpF"
                     /locus_tag="OHN28_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCQ2299653.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            177..1850
                     /gene="kdpA"
                     /locus_tag="OHN28_004378"
     CDS             177..1850
                     /gene="kdpA"
                     /locus_tag="OHN28_004378"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCQ2299654.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1873..3921
                     /gene="kdpB"
                     /locus_tag="OHN28_004379"
     CDS             1873..3921
                     /gene="kdpB"
                     /locus_tag="OHN28_004379"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCQ2299655.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3930..4502
                     /gene="kdpC"
                     /locus_tag="OHN28_004380"
     CDS             3930..4502
                     /gene="kdpC"
                     /locus_tag="OHN28_004380"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCQ2299656.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            4495..7179
                     /gene="kdpD"
                     /locus_tag="OHN28_004381"
     CDS             4495..7179
                     /gene="kdpD"
                     /locus_tag="OHN28_004381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCQ2299657.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7176..>7817
                     /gene="kdpE"
                     /locus_tag="OHN28_004382"
     CDS             7176..>7817
                     /gene="kdpE"
                     /locus_tag="OHN28_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCQ2299658.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFI"
BASE COUNT         1602 a         2066 c         2327 g         1822 t
ORIGIN      
        1 ttacaccacg cccgcagatt tttgcgaaat ctttgcagcc agaactctac ccttccggta
       61 tcacttttag gccactggag gtgcactgtg agtgcaggcg tgataaccgg cgtgttgctg
      121 gtgtttttat tactgggtta tctggtttat gccctgatca atgcggaggc attctgatgg
      181 ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg ctggcgcgtc
      241 ccttaggtag cgggctggcg cggctgatta atgatattcc tcttcctggt acgacgggcg
      301 ttgaacgcgt actctttagc gcgcttggcg tctctgaccg cgagatgaac tggaagcagt
      361 atctctcagc cattcttggc ctgaatatac tggggctggc ggtgctgttt tttatgttgc
      421 tcggtcagca ctatctgccg cttaatccac agcagttgcc agggctgtcg tgggatctgg
      481 cgctcaatac cgccgtcagc tttgtcacca ataccaactg gcaatcttat agcggtgaaa
      541 ccacgttgag ctatttcagc cagatggcgg gcttaacggt acaaaacttt ctttctgccg
      601 ccagcgggat tgcggtgatt tttgccctca ttcgtgcatt tacccgccag agcatgagca
      661 cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgcta gtccctgtgg
      721 cgttgttgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt ctgccctatc
      781 aggcggtgaa taccgttgaa ggagcacaac agctgttacc catggggcca gtagcttctc
      841 aggaagcgat caaaatgctc ggaactaacg gcggtggctt ttttaatgcc aactcgtcgc
      901 atccgtttga aaacccaacc gcactgacca acttcgtgca aatgctggcg attttcttga
      961 tcccaacagc gctgtgcttt gcctttggtg aagtggcggg cgatcgtcgc caggggcgca
     1021 tgttgctgtg ggcgatgtcg gtgatttttg tcatctgcgt aggcgtagtg atgtgggcag
     1081 aagttcaggg taatcctcat ctgctggcgc tcggcgcgga cagcagcatc aatatggaag
     1141 gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg actacggcgg
     1201 cttcctgcgg cgcggtgatt gcgatgcatg attcgtttac cgctcttggt ggcatggtgc
     1261 cgatgtggtt gatgcaaatt ggtgaagtgg tattcggcgg cgtcggttcc ggcctttacg
     1321 gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt ggtcgtacac
     1381 cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgactgca ctggcgattc
     1441 tggtcacccc gacgctggtg ctgatgggcg cggcgctggc aatgatgacc gacgccggac
     1501 gtagcgccat gctcaaccct ggcccgcatg gttttagcga agtgctgtac gccgtgtcat
     1561 ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct ccattctgga
     1621 actgtttact ggcgttgtgc atgtttgtcg ggcgcttcgg ggtgattatc ccggtgatgg
     1681 caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc acattgccaa
     1741 cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt ggcgcactga
     1801 cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga tgatattgag
     1861 tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact tgtcgttcag
     1921 gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa tccggtgatg
     1981 tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat ggcaagcggt
     2041 gtgatgcctg gtaatgcgct gtttagcgcg gcaattagcg gctggctgtg ggtcaccgta
     2101 ctgttcgcta atttcgccga ggcgttggca gaaggccgca gtaaagcgca ggccaacagt
     2161 ctgaaagggg tgaaaaaaac cgcttttgct cgcaagctgc gcgaaccgaa atatggcgct
     2221 gtggcagaca aagttcctgc cgatcaactg cgtaaaggcg atatcgtact ggtagaagct
     2281 ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt cgatgaaagc
     2341 gccatcaccg gggaatcggc tccggtgatc cgtgaatccg gcggtgattt tgcctccgtc
     2401 actggcggca cgcgtattct ttctgactgg ctggtgattg agtgcagcgt taaccccggc
     2461 gagacatttc tggatcggat gatcgcgatg gtggaaggtg cgcagcgacg taaaacgccg
     2521 aacgagattg ccctgaccat tctcctgatt gccctgacta tcgtcttttt actggcaacc
     2581 gccacgctgt ggccgttttc cgcgtggggc ggtaatgcag tcagcgtaac ggtactggtg
     2641 gcactgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc gattggcgtc
     2701 gccgggatga gtcggatgct gggcgcgaat gttattgcca ccagcgggcg tgcagttgaa
     2761 gcggcgggcg acgtggacgt cctgctgctg gataaaaccg gcaccatcac actcggtaac
     2821 cgtcaggcgt cggagtttat ccctgcgcag ggcgtggatg aaaaaacgct ggctgacgcc
     2881 gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc gcagtattgt gatcctcgcc
     2941 aagcagcgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac ctttgtaccg
     3001 tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat ccgtaaaggt
     3061 tctgtcgatg ccattcgccg ccatgttgag gctaacggcg gtcacttccc tgccgatgtt
     3121 gatcaaaaag tcgatcaagt tgcgcgtcag ggagccacgc cgctggtggt ggtggaaggt
     3181 tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat taaagagcgc
     3241 ttcgcccagc tgcgtaaaat gggcattaaa acggtgatga ttaccggcga taaccgtctg
     3301 actgccgccg cgattgccgc ggaagcgggc gtcgatgatt ttctcgccga agcaacaccg
     3361 gaggccaagc tggcgttgat tcgtcagtat caggcagaag gccgtttagt ggcgatgacc
     3421 ggcgatggca ccaacgatgc tccggcgctg gcgcaggcgg atgtcgcggt ggcgatgaac
     3481 tccggcaccc aggcggcgaa agaagcaggc aatatggtgg atctcgactc caacccgacc
     3541 aagttgatcg aggtggtgca cattggcaaa cagatgctga tgacccgtgg ctcgttgacc
     3601 accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccagc ggcgttcgcg
     3661 gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc cgactccgcc
     3721 attctcagtg cggtcatttt caacgccttg attatcgtct ttttgattcc cctggcgtta
     3781 aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa cttatggatt
     3841 tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga tttactgcta
     3901 accgtttgcg gtctggtgtg aggtttacca tgagtggatt acgtccggca ttatcgacat
     3961 ttctctttct gttattgatt actggcggcg tttacccgct gctgaccacc gcactggggc
     4021 aatggtggtt tccctggcag gccaacggtt cattgattcg tgaaggtgat acggtgcgcg
     4081 gttcggcatt aatcgggcaa aattttaccg gcaacggcta ttttcatggt cgcccgtcgg
     4141 caacagcaga aatgccctat aatccacagg cttctggcgg gagcaatctg gcagtcagta
     4201 acccggtgct ggataaacag atagccgcac gcgttgccgc attacgggcc gctaacccgg
     4261 acgccagcac gagcgttccg gttgaactgg tgacagcgtc agcaagcggg ctggacaata
     4321 atattacccc gcaagcggcg gcctggcaga tcccacgcgt ggcgaaagcg cgtaatctca
     4381 gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca acaaccgctg gtgaactata
     4441 tcggccagcc ggtcgtcaac gttgtagaac tcaatctggc gctggataag cttgatgaat
     4501 aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc gccgcatcgg
     4561 gggaagctga aagttttctt cggtgcctgc gcaggcgtcg gaaagacctg ggcgatgctg
     4621 gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt ggtagaaacc
     4681 cacgggcgga aagataccgc cgcaatgctg gaaggtctgg ctgttctgcc gctgaaacgc
     4741 caggcgtacc gtgggcggca tatcagcgag tttgatctcg atgccgccct cgcccgccgt
     4801 ccggcactga tcttaatgga cgaactggcg cacagtaatg cgccaggttc tcgtcatcct
     4861 aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt cactaccgtc
     4921 aacgttcagc atctggaaag cctgaacgat gtggtcagtg gcgtaaccgg aattcaggtt
     4981 cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtagtact ggtggacttg
     5041 cccccggacg atctgcgcca acgactgaaa gaaggcaaag tctatattgc cgggcaggca
     5101 gaacgcgcca ttgagcattt tttccgcaaa ggcaatctga tcgccctgcg cgagctggcg
     5161 ctacgccgta ctgccgatcg cgtagatgag caaatgcgcg cgtggcgggg gcatcctggc
     5221 gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcgggca taacaccggc
     5281 agtgagaaac tggtccgcgc ggcggcgcgg ctggcgtcgc gcctgggcag tgtctggcac
     5341 gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg tcgggcaatt
     5401 ctcagcgcct tacgtctggc gcaggagttg ggcgcggaga cggcaacact ttctgatcca
     5461 gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc
     5521 ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg gctggcgcgc
     5581 atcgcccccg atctcgatca ggtactggtc gcgcttgatg aaccacccgc ccgcacgatt
     5641 aataacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc
     5701 gtggttgccg ccgcgttatg cgccgttatc accttaattg ccatgcagtg gctgatggcg
     5761 tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggttgtggt ggcactattt
     5821 tacggacgct ggccttcggt ggttgcgacc gtcattaatg tggtgagttt cgatctcttt
     5881 tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgcg
     5941 gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg
     6001 cgggtagccc gttaccgcga acaacgcaca cggcacttat atgaaatgtc caaagctctg
     6061 gctgtgggcc gtagttcgca ggatatcgcc gccaccagcg aacagtttat tgcctccacg
     6121 tttcatgccc gtagccaggt gttgctgcct gatgacaacg gtaaattgca gcctttaaca
     6181 catccgcaag gaatgacgcc gtgggacgat gccatcgcgc agtggagttt tgataaaggc
     6241 ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat tttgccgcta
     6301 aaaagcggcg agaaaaccta cggactggtg gtggtggaac cggggaatct gcgccagttg
     6361 atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc caatgccctt
     6421 gagcgactga cactgaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag
     6481 atccgcaacg ccctgctggc ggcgctttcg catgatttac gcacgccgct tacggtgctg
     6541 ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc
     6601 caggccagcg agattcgtca gcatgtgctg aacaccaccc gactggtgaa taatctactg
     6661 gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa
     6721 gaagtggtcg gcagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc catcaatctt
     6781 tctctgccag agccgctgac cttaatccac gttgacgggc cactctttga acgggtgctg
     6841 attaacctgc tggagaacgc ggtgaaatat gcgggggcgc aggccgaaat tggtatcaat
     6901 gcccacatcg agggcgaaaa tctacaactg gatgtctggg ataacggccc cggtcttcca
     6961 ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcggta
     7021 ccgggtgtag ggcttgggtt ggcaatttgt cgggcgatag tggatgtaca cggcggcacc
     7081 attaccgcat tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa
     7141 acagcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg attgttgaag
     7201 atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgatggg atgcgcgtct
     7261 ttgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga
     7321 ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc
     7381 agtggagcgc ggtgccggtg attgtgcttt ccgcacgtag cgaagagagc gacaaaatcg
     7441 ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc
     7501 aggcccgtct gcgcgtcgct ttacgtcgcc actccgccac cgccgcgccc gatccgctgg
     7561 tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt tatccatcgg ggcgaggaag
     7621 aggtgcatct caccccgatt gagttccgcc tgctggcagt gttgctcaac aacgccggaa
     7681 aagtgctcac ccagcgccag ttactcaatc aggtgtgggg accaaacgcg gtcgaacaca
     7741 gccactattt gcgtatttat atggggcatc tgcgacaaaa actggagcaa gatcccgccc
     7801 gcccccgcca ttttatt
//
LOCUS       DAJOTB010000198         7805 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJOTB010000198 DAJOTB010000000
VERSION     DAJOTB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.198"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1223..1789
                     /locus_tag="OHN28_004383"
     CDS             1223..1789
                     /locus_tag="OHN28_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2299659.1"
                     /translation="MLKSYTPNWFFTALLDHHIDQLVARYSCLRALRMDFFYRKETPD
                     FLQPDHRRLELQLRMLLEQVEQFENIVGFFWVIEWTVDHGFHAHAVFWIDRQRVKKIY
                     PSAERITECWRSITHNSGSAHRCTYQPHYAYNINIPVRHNEPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            2036..2611
                     /locus_tag="OHN28_004384"
     CDS             2036..2611
                     /locus_tag="OHN28_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299660.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPWEHDNAFNSRQTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRDVVLDSRSPDCENILKELIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
     gene            2680..2913
                     /locus_tag="OHN28_004385"
     CDS             2680..2913
                     /locus_tag="OHN28_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2299661.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2910..3068
                     /locus_tag="OHN28_004386"
     CDS             2910..3068
                     /locus_tag="OHN28_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299662.1"
                     /translation="MKRVAMPALVLRKSALSLVLATLPFIFLLTLSGQPADCSSFLTD
                     SSFHSHCL"
     gene            3156..3704
                     /locus_tag="OHN28_004387"
     CDS             3156..3704
                     /locus_tag="OHN28_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299663.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTACPSSPVVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASATVDEECSQRVDLLQQTWKVISAGGQCQVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            complement(3723..4067)
                     /locus_tag="OHN28_004388"
     CDS             complement(3723..4067)
                     /locus_tag="OHN28_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HCQ2299664.1"
                     /translation="MTKRQRKNRKRINRLVEHWPELFNREKPQPRKVEIPDDLIQDIA
                     IRELAFSAGALRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     VLNDRQKARKPR"
     gene            complement(4064..4237)
                     /locus_tag="OHN28_004389"
     CDS             complement(4064..4237)
                     /locus_tag="OHN28_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCQ2299665.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKMNTAHNVKARERIPQLNDK
                     QSPALLKKHRGGI"
     gene            4373..4546
                     /locus_tag="OHN28_004390"
     CDS             4373..4546
                     /locus_tag="OHN28_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299666.1"
                     /translation="MTRPAGSLDKRTISANTTVPGEVKSFLLAQFLKKWRRTKMGRHT
                     WKVSLPDDAKKQK"
     gene            4960..5394
                     /locus_tag="OHN28_004391"
     CDS             4960..5394
                     /locus_tag="OHN28_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCQ2299667.1"
                     /translation="MSKKKISVENEEFITYRALLLSIDFVKKLVTEMSDEDLNKVKEN
                     FDIAIQQRDERKENMKAIKSLMDEHGISLEDFCREFGKKSQAGRKSVSGGHKYEYDDN
                     GKRMYWNGQGRRPKAIAEAIKAGGSLDDFLISQEVSSGPDYE"
     gene            complement(5811..6380)
                     /locus_tag="OHN28_004392"
     CDS             complement(5811..6380)
                     /locus_tag="OHN28_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299668.1"
                     /translation="MGKNNYPENKYRKWFSTAITYLITGGLCWYMLWASAISHYTVLQ
                     NTVPEWTTYFCTGIITGSLSFIYAFYIRTFNRTLKSLLNAFTGGFCLGFILSLNCYDV
                     CVYLFPDKVISYESEYDVVFPGPSRGKYGHCEAGLWLKDQNTSRWIQLCTNKEFLRSH
                     HKQGMTGVWVTARVNKIGSYIVKYEFIYM"
     gene            complement(6480..7493)
                     /gene="dgt"
                     /locus_tag="OHN28_004393"
                     /pseudo
     CDS             complement(6480..7493)
                     /gene="dgt"
                     /locus_tag="OHN28_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
     gene            complement(7545..>7805)
                     /locus_tag="OHN28_004394"
     CDS             complement(7545..>7805)
                     /locus_tag="OHN28_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCQ2299669.1"
                     /translation="LLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSM
                     KTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2100 a         1711 c         1703 g         2291 t
ORIGIN      
        1 tatataactt cgtttgattt tcgtcgtgat gagtgcaggt gtatatgata atgtcggttt
       61 ctgcctgaat aacgaatggt tatcactgtt actgaaaaga gatgctgttc aagtttgcat
      121 ttcaatagga gagtgaagaa aaatgatttg atattttttc tgtaagataa tggtttgtca
      181 tgttcgtatt cagaacggat gatttcttat ttttactcat caaacatgga taattttaat
      241 ttaggtttat tactataatt agtatctacc cctttattta tcatccatct ctgcccttca
      301 ttttccctct ctgtctttac acattccatt actcttattc tcatccagtt atattcactc
      361 ccttccttgt ctaatcttca ttgtcaccat tatcatggta tccaccttct ccattcctca
      421 atcataacta ctgtaactca tatgatactc agataagtta ttctctacta cgctattgct
      481 gatatccatc tgttcctgtt ttcagtaaaa taataattta ttttaatttt atccatcatg
      541 agctgtattt gcttaacaat gattgtttat ctgatatgtt ttaaccattc tggtaaatat
      601 caatacttct gtcatcactg attagccgaa tgcttaccct ttgataaggt atccatgatt
      661 ctgtaacgat taaccgagca gggtaaacaa cagaatcaac agagtgaatt cctgattatg
      721 tttgtttcgg gttgtattgt ttaagatgca gggtaaaaat catgtcagtg gtgtttttta
      781 ctctggtaat gtgtgctgtt aacgttcatg tccttaagaa cagattaaat gagtgcgtat
      841 aagtttattg cgttggcctt gtgcacggtt tttagaacca ctgagagcaa gtttgtagtt
      901 tatgatggtg attagtcgta atatgtttct tgaacttctg aatgtgggtt tttatcgcgt
      961 gttttgcagt atttcgtgat gtttattgat tctgtatttt attcgctact cgtttatctc
     1021 atctctttag caaataccat cagataatcc atttctttct gcataatgct gcgtatcgtt
     1081 aataacccgt cgtatccatt ctgctacagc atgcctgata aataccatct gtaagttatt
     1141 accgttttag atctgattat gagcgaaagc attaattcgc tcacagagct taaaacatca
     1201 ttaactttca ggagtcatca acatgcttaa atcttacaca ccaaactggt tttttaccgc
     1261 tttacttgac catcacatcg atcagttggt cgcacgctat tcctgcctgc gggccttacg
     1321 catggatttc ttctacagga aagagacgcc tgatttctta caacctgatc atcgccggct
     1381 tgaattgcag ttgcgtatgc tgctggagca ggttgaacag tttgaaaata ttgttggctt
     1441 cttctgggtg attgaatgga cggttgatca tggttttcat gcgcatgcgg ttttctggat
     1501 cgatcgtcag agggttaaaa aaatatatcc cagtgcggag cggattacgg aatgctggcg
     1561 gtctattacg cataacagcg gttcggcaca ccgctgtaca tatcagccgc attatgcata
     1621 caacatcaac attcctgtgc gccataacga gcctgaaagc atcgataata ttcgcggtgc
     1681 cctgcattat ctggcgaaag aagaacaaaa agacgggctg tgtgcctacg gctgcaatga
     1741 agttcctgaa cgtcctgctg caggacgacc acgtaagcct cacttctgaa gcttaaggcc
     1801 tgagcctccg ctcctggaaa cactccgtcg gtaaaaactt accgcattga ttaatgatgt
     1861 gaactgaagt caacggagat cattcatcct gaacctgaat ccggtgttct gtcccttatc
     1921 ttcccgttct gcttcagttc ttcacttatt ccatcaatct catccgcaag ccataacacg
     1981 tcagctcatt cacgggcagg acgcattgtg ggctgcgcat aacggaacat atcttatgaa
     2041 tgctattcct tatttcgact atagcctggc acccttctgg ccatcttatc agaacaaagt
     2101 catcggcgtc cttgagcgtg cgctgcgtga gcagtccggc tcacggatac ggcggatcct
     2161 gcttcgtctg ccgtgggaac atgacaacgc cttcaacagc agacagacct ggtttggtat
     2221 ggattttatc gaaaccgtca gtgcgctgat gaatgcgaaa cccggacgcg acctttgctg
     2281 gctcctgacc cgtcatccgg aaaagccgga ataccacgtg gtgctgtgcg tcagacagga
     2341 gtatttcgac ggccctgaac tggaccggct gattctggat gcctggagca acgtactggg
     2401 cttcgcgtca ccgggtgaag caaagcagta tcagaagcag atcacccggg atgtggtgct
     2461 ggacagtcgt tcaccggact gtgaaaacat cctcaaagag ttgatatggg cgttcagtga
     2521 tttcgcccgc gatcgtcgtg gtgtgtgcga tccggaagcc cgttgcctcg ccggtaatcc
     2581 cggctatccc ggatctgcag gaccgttctg aaagctcggt tcagcatctt tccctgcatt
     2641 cgcacaaact tcataaatct cacaaaggac attctgacca tgttgaccac aacaagccac
     2701 gacagcgtgt tactgcgtgc cgacgatccc ctgatcgaca tgaactacat caccagtttc
     2761 accggtatga ccgataaatg gttttacaag ctgatcagtg aaggtcattt ccctaaaccc
     2821 atcaaactgg ggcgcagcag ccgctggtac aaaagtgaag tggagcagtg gatgcagcag
     2881 cgaattgagg aatcacgagg agcagcagca tgaaacgtgt tgcaatgccg gcgctggtat
     2941 tgcggaaatc agccctgtcc ctggtgctgg cgacattacc gttcatcttc ctgctgaccc
     3001 tctccggtca gccagcagat tgtagctctt tcctgactga ttcgtcattc cattcacatt
     3061 gtttataact ggcattacac cggtgttgat gcgcaccttc ccgtgtgtct gcaccggctg
     3121 aacaaaattc aacagggttt gaaaaggaac atttcgtgca aataaccgaa gccttaattt
     3181 cagagccggg agacatccgg cgttttgttc agcaggcggt ggaccactgg ccaaatctgc
     3241 tggcatttca cttcacgctc tattcggcag aaggaaacat caacgggcaa cagattcatg
     3301 cattctgtac ttccttttac cgacaggtgc acgaacgtat tactgagcgt aatcacactg
     3361 cctgtccatc ctcgccggtg gtattacgct ggttgcggga acaacatgga ggagcaacaa
     3421 tacgatgcct gttgctgctc agccagacga gcatttgcca tccgcgggcc agtgccacgg
     3481 ttgatgaaga atgttcgcaa cgggtggatt tattgcaaca gacctggaag gtgataagcg
     3541 ctggcggaca atgccaggtt gaaaggtgtt ttcgggttgc ccggggtgat acatccggtc
     3601 agtatgtagc gttaaaaacc gtcgcattgt ctctggggtt accggttgtg accgccatta
     3661 cccatcgtcc ggtacagcgc tgtacattga ttacagctca gtgaatcagc gcagttgtgt
     3721 agtcaacgag gttttctggc tttttgtcgg tcattcagca ccattagccg ggtctctgcc
     3781 tctttctgtt cctgtggagt cacttccccg cacggctgcc ccttcaggtc gtaacgggcg
     3841 ccgccagcca ttaacgcacg gtaatagcgc ggagactgca cataagacgc tactgccgcg
     3901 cgtaatgcac ctgcgctaaa cgccagttct ctgatggcga tatcctgtat caggtcgtca
     3961 ggtatctcaa ctttacgcgg ttggggtttt tcccggttga ataactcagg ccagtgttcc
     4021 acaagacgat taatacgctt acggttttta cgctggcgtt ttgtcatatg ccgccacggt
     4081 gttttttcag cagagcaggg ctctgtttat cgttcaactg tggtatccgt tccctggcct
     4141 tcacgttgtg tgcagtgttc attttatcct gcagcggcgt tgccggtgat ttactgaaaa
     4201 tgccctgtgg tttacggtta atggtcagcg ttaccatcag tattgttacc tcatgctgtc
     4261 aatggaagta gtaccccatc cagagccttg gcgaccccgg ggctgaaaac gaaacaataa
     4321 cccgcgcgca gtgcgaggag cccgggtgtg aagcaatcag caggtaatat taatgacaag
     4381 accagcaggt tctttagaca aacgaacaat atcagcaaat acgacagttc caggagaggt
     4441 aaaaagtttt ttacttgcac aattcttaaa aaaatggaga cgaacaaaaa tggggcgaca
     4501 tacctggaaa gtatcgctac cagatgatgc taaaaagcag aaatagtgtt ccaggcctga
     4561 aagggatgag aatcagtatc tgtgtgtatt tataacatct tcttgaagag gctggagatt
     4621 gttagtccat tagtataatc gatattcatt ttcctgcgaa tatgcaggct acaccagata
     4681 tatgattgct accgaatttc atcagtttga tgaaattacg catcatactg cgggttttct
     4741 cattaacatt aacgaaagtg aatggactca gacaagtagt gaccggcgca atttcgctaa
     4801 gtatcaaata caaagaacgt tctcttactt tctgcgatgg cctttccttc ggaaaggaag
     4861 tgacgattaa gtgtcgaaaa atgtgataca tatattattt ttatggcaaa tcacctgata
     4921 ggatttgcca taaatgaatg aaaagctgag tgttaataaa tgagtaagaa gaaaatttct
     4981 gttgaaaatg aagagttcat tacttaccgg gcattacttc tgagtattga ctttgttaaa
     5041 aaactggtca cagaaatgtc tgatgaagat ttaaataagg ttaaggaaaa ctttgacata
     5101 gctattcagc aacgtgatga gcgaaaggaa aatatgaaag ctatcaaatc actcatggat
     5161 gaacatggta tatctctgga agatttttgt cgtgaatttg gcaaaaaatc acaggcaggc
     5221 agaaaaagtg tgtcaggagg gcataaatac gagtatgacg ataacggtaa aagaatgtac
     5281 tggaatgggc aaggacgtcg gcctaaggcc atagcagagg ctataaaagc cggaggttct
     5341 cttgatgatt ttcttatatc tcaggaagta tcatctggtc ctgattatga ataacagcaa
     5401 tatatatttg aatttgtatt aatatcaagg aatatatagc aagcacttct ccggagttga
     5461 tatattatcg acagggaatg aaaatgcctg acttcatgct aatatatggc aataaagata
     5521 aatgagttat actgtagagt tatgaaataa cgatagtgat aagagccagt cagcgagact
     5581 gattgtttta tctgtactgg cagcaggcag ttaaccatgg cttactgctt cagcttctga
     5641 atctgtgttg atgatattta tctgtttata aggcaaggct tgtctctgtg agttggtaat
     5701 ctttatcccg ggatagcttt tctctgtatt tttagtatag ccagtaatcg attactcctg
     5761 tatgcgcaaa aatatagcaa ctcatccatg gtaaacatgt cagcttttac ctacatatag
     5821 ataaattcat atttcacgat atacgaccca attttattaa cacgagctgt tacccataca
     5881 cctgtcatcc cctgcttatg gtgactgcgc aggaactctt tatttgtaca aagttgtatc
     5941 catctggaag tgttttgatc tttcaaccag agtcccgctt cacaatgtcc atactttcct
     6001 cttgatggtc ccggaaaaac aacatcatat tcagattcat agctaataac cttatccgga
     6061 aaaagataaa cacagacatc gtaacagttc agactcagta taaatccaag acaaaatccc
     6121 cccgtgaatg catttaataa agactttaat gttctgttaa aggtgcggat ataaaatgca
     6181 taaataaaag aaagagatcc ggtgattata ccggtgcaaa aatatgttgt ccattcaggt
     6241 accgtatttt gtaaaacagt ataatgagat atagcggatg cccacaacat ataccagcac
     6301 aaaccacctg taataagata tgttattgct gtggaaaacc atttcctgta tttattttca
     6361 gggtaattgt tcttgcccat caacgcattt ccagaatatt taaaataacc tgtcaccgaa
     6421 acggaataaa ggcgcaataa ccataacggg aaaatatgcg cctctggtaa tcggcagtgt
     6481 tacttcagac agtgagtttc ccattccttg actgcaaatg tatccatcat acctgaaata
     6541 tagtcaacca ctgctcgttt tttgctgatt tcaaatatac agaaatctgg ttggggtaag
     6601 gtgaatttaa gttcaggagg cagaaattta taattctctc cttcattgta aaagtcatat
     6661 aaatccctga ttattttatt tcccctgaac tcataggtga ggatattacg ttttcgcatg
     6721 atgactttaa agagaagttt tttgagtcct tcggataacg cattcagttt cccgaacccc
     6781 agttcctgaa aaccattcag gttcgttact acagaaatat ctgcaacgag gcgattgaca
     6841 atatttgaag ttaactcctt gcggaatatg atggcaaatt cttctgaggt tttcagtaag
     6901 ttagcctgaa aagcggtgtt tctcgactga gtaacgattt ccctgaactg ttccctcgct
     6961 ccccggaatt cgtcagaaat ctgaaactca tattcaatat cttcaatcgt gaccatgttc
     7021 ctgctaaggg catcttccag gtcatgcgcg gcgtaggcaa tctcatccgc gagatccatt
     7081 atctgcgcat caatcgtttt ctctccggga ttcagatcga gctgattttc agcaagcagg
     7141 ttaatgtaaa aatagtaatc atcatcataa atgaacttgc cggcagtagc tgcagcgcgg
     7201 gggatgggat acttaacaac cgaaagtaaa ctacggtgag tcagatttaa tccgttgcag
     7261 tatgaaaatt tcttctccag ttttctcagg atacgtagcg cctgtgcatt accttcatag
     7321 ccgccgattt cttcagacag ttcattaagc agtttttcac cggaatgacc gaaaggagga
     7381 tttccgatgt catgcgccag cgctgccagt tcaaccacca ccgggttaat cagtttgagg
     7441 tctgacgcga tacttcgggc gatctgagca acctcaagac tgtgcgttaa tcggcactgt
     7501 tgcaaagtta gcgatgaggc agccttttgt cttattcaaa ggccttacat ttcaaaaact
     7561 ctgcttacca ggcgcatttc gcccagggga tcaccataat aaaatgctga ggcctggcct
     7621 ttgcgtagtg cacgcatcac ctcaatacct ttgatggtgg cgtaagccgt cttcatggat
     7681 ttaaatccca gcgtggcgcc gattatccgt ttcagtttgc catgatcgca ttcaatcacg
     7741 ttgttccggt acttaatctg tcggtgttca acgtcagacg ggcaccggcc ttcgcgtttg
     7801 agcag
//
LOCUS       DAJOTB010000199         7663 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJOTB010000199 DAJOTB010000000
VERSION     DAJOTB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.199"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(86..211)
                     /locus_tag="OHN28_004395"
                     /pseudo
     CDS             complement(86..211)
                     /locus_tag="OHN28_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            253..483
                     /locus_tag="OHN28_004396"
                     /pseudo
     CDS             253..483
                     /locus_tag="OHN28_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            535..1470
                     /gene="ttcA"
                     /locus_tag="OHN28_004397"
     CDS             535..1470
                     /gene="ttcA"
                     /locus_tag="OHN28_004397"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCQ2299670.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1598..2971)
                     /gene="dbpA"
                     /locus_tag="OHN28_004398"
     CDS             complement(1598..2971)
                     /gene="dbpA"
                     /locus_tag="OHN28_004398"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCQ2299671.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3449..4432)
                     /gene="zntB"
                     /locus_tag="OHN28_004399"
     CDS             complement(3449..4432)
                     /gene="zntB"
                     /locus_tag="OHN28_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCQ2299672.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4687..5919
                     /gene="dgcM"
                     /locus_tag="OHN28_004400"
     CDS             4687..5919
                     /gene="dgcM"
                     /locus_tag="OHN28_004400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCQ2299673.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5940..6503)
                     /gene="smrA"
                     /locus_tag="OHN28_004401"
     CDS             complement(5940..6503)
                     /gene="smrA"
                     /locus_tag="OHN28_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCQ2299674.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6698..>7663)
                     /locus_tag="OHN28_004402"
     CDS             complement(6698..>7663)
                     /locus_tag="OHN28_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2299675.1"
                     /translation="NFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDN
                     TIFLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGGVGIFSTVYL
                     IPVFLGRVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSM
                     YLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRN
                     LGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRGGSDQTSEI
                     LASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         2093 a         1946 c         1754 g         1870 t
ORIGIN      
        1 attcaccgaa aggtttcaca aaatatcccc tgatagaaaa ctaaaaaaca ctgcataata
       61 tcgctattct ggcacttgcg aaaaattata ttctgcacct gttgtagcgt tctatttctc
      121 tgcctggcaa cgaatgcaca atgtccgcca cagtttctgt tctaatttct tcctgtgtta
      181 ccgcatcctg tacgggtagc gcaacaccag catgctaagc aaggctttcc catcagattt
      241 cactacgact atttgggagc gagatggaat gcagcagtaa aacgtgctgg tattcgccgc
      301 cgtaatccgt accatacgcg acatactttt gcctgctggc tgttgacggc aggagcgaac
      361 ccggcattta tagccagcca gatggggcat gaaactgcgc agatggtgta tgaaatttac
      421 ggtatgtgga ttgatgacat gaacgacgaa caggtagcca tgttgaatgc gcggttatcg
      481 tagttgcaaa gtttgccctc aatatgcccc atttagtacc agagaactga aataatgcaa
      541 gaaaatcaac aaattacaaa gaaagaacaa tacaacctga acaaattaca aaaacgtctg
      601 cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg cgatcgcatc
      661 atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct gcgcaatttg
      721 cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca aaagcaaccg
      781 ggcttcccgg aacacgttct gcccgagtat cttgaaacgc tgggcgttga gtacaagatt
      841 gttgaagaga acacttacgg tatcgtgaaa gagaagattc cggagggcaa aaccacttgc
      901 tcactgtgtt ctcgtcttcg tcgcggtatc ctttatcgca ccgcaacgga actgggggcg
      961 acgaagatcg cgctgggtca ccatcgtgac gatatcctgc aaacgctgtt cttaaatatg
     1021 ttctacggtg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga tggcaaacat
     1081 atcgtgattc gtccgctggc ctactgccgc gagaaagata ttcagcgctt tgccgatgca
     1141 aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct gcaacgtcag
     1201 gtaattgctg acatgttgcg tgactgggat aaacgttatc cggggcgtat cgagacgatg
     1261 ttcagcgcaa tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct gttcgatttc
     1321 aaaggcatta cccacggttc tgaagtggtt aacggggggg atctggcgtt tgatcgcgaa
     1381 gagatcccac tacaaccagc gggctggcag ccagaagaag atgaaaatca gttggatgag
     1441 ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatacg caagcgtatc
     1501 gggctactct tatggaagcc gataagacgc ggccagcgtc gcatccggca atgccgaaga
     1561 agatgtttac tcttgcaccc tgcaattcaa catttcatta ttttaataac cgcacccggc
     1621 acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga gcaacagcct
     1681 gacgcaccgc gacataaaca tgcgccggat gcacggcaat tttgccaata tctgcgccat
     1741 caagcccgat gtctcctgtc agcgcaccta atacatcacc cgggcgcatt ttggcttttt
     1801 tcccgccatc gatacacaac gttgccattt ctgcttccag cggcacaatg gaactattcg
     1861 ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata ttggcccgct
     1921 gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca cgagctgtac
     1981 gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc accaccagct
     2041 caagcgattt tatatccaga ccacgcgcag caacatcagt cgcgaccagt acacgggcgc
     2101 taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga tcgccgtgta
     2161 acgacaatgc actttgccct acttcattca gcgcgtcgca gacagactgg caatcttttt
     2221 tggtattgca aaacaccacg caagaggatg gctgatgcaa gcttaataac cgttgcaaca
     2281 gaggaatttt gcctttgctg gatgtctcat aaaattgttg ttcaataggt ggcagagcat
     2341 ctgttgagtc aatttcaatc gctaaaggat cgcgctgcac tcttccgctg attgcggcaa
     2401 tggcttccgg ccaggttgcc gaaaacagaa gtgtctgccg agatgctggc gcaaaacgga
     2461 tgacatcatc aatggcgtca ctaaatccca tatccagcat gcggtcggcc tcatccatca
     2521 ccagcgtatt caacgcatcc agtgatacag tgcctttttg caggtgatcc agcaaacggc
     2581 ccggcgttgc cacgataata tgcggcgcat gttgtaacga atcacgctgc ataccgaacg
     2641 gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca
     2701 attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa gcctgggttt
     2761 gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc
     2821 tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga agcgcggcgg
     2881 cctgcaccgg cgtcatggtt aaataaccca acccattaag gtttgtgagt tgggcgggag
     2941 gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat cgacgcaaag
     3001 tcagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggctcttc
     3061 atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca cgggaacagg
     3121 atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc
     3181 ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg caaaaaaaag
     3241 ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg
     3301 aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagagag
     3361 atattgccct gaatgggtag agagtttatt gacttcgctc aaactttacg gcgtttttgt
     3421 atacagacag ccgggaaaat tgcttttgtt acaaccattt actacgatgc aaccataaag
     3481 caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccatc
     3541 caccgccagg gatcccacca aggttgacgc caaataaccc tgtcagaaac gtactgggta
     3601 aaaagaccat tgccatcaac gacattgtat aagtacgacg agctaaattt tcctgcatca
     3661 cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa
     3721 gaccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca tcgctcatcc
     3781 acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac
     3841 gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat
     3901 cgagaagatt atcttcgagg tcgataattt tatcgtgcag ctgctcgata aattcgctgg
     3961 aatgatcggt caacgcatcg cacacatcaa ccagccaccc cccgcaatcg gtcggaccag
     4021 tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt tgtcgggtcg
     4081 aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat
     4141 cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaga cggctgactc
     4201 ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc agcggtgttg
     4261 tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag cagggatgcg
     4321 cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc
     4381 atgcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc acagtgcctc
     4441 catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca
     4501 ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg
     4561 cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct
     4621 gatcatatga cgtggctttt ttgcgatcgg atagcaacaa caattgataa aaataatggg
     4681 atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatc
     4741 gtttcgccct ttgaggaaca gttaatttat gccaacagcg aggcgcgact gttgatgcaa
     4801 gacctcacgt ttagtcagct acgaaccggt ccctattccg tctcctcaca aaaagaactg
     4861 ccgaaatacc tctccgatct gcaaaatcaa cacgatatta tcgaaatcct caccgttcag
     4921 cgcaatgaag aggaaacagc attaagctgt cggcttgttt tgcgagagct gacagaaaca
     4981 gaaccggtga ttattttcga aggtatcgaa gcaccggcaa cgctgggttt aaaagccagt
     5041 cgctcgacaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac
     5101 tctgcgccaa tgttgttgat tgacccctca cgagatggcc aaatcgttga tgctaacctc
     5161 gccgctctca atttctatgg ttataaccat gaaacgatgt gccagaaaca tacctgggaa
     5221 ataaatatgc tcgggcgtcg cgtcatgcct atcatgcatg aaatctcgca tttacccggt
     5281 ggtcataaac ctttgaattt tgttcataaa ctggcggatg gttcgactcg tcatgtgcag
     5341 acctatgccg ggccgattga aatttatggc gacaagctca tgttatgtat tgtgcatgat
     5401 attactgagc aaaaacggct ggaggagcag ctggaacatg ctgcccacca tgatgcgatg
     5461 acaggattac tgaatcggcg acagttttat cacattaccg agccaggcca aatgcagcac
     5521 ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt
     5581 aacgatctct atgggcattc taaaggtgat gaggtgttat gcgcccttgc ccgcactctc
     5641 gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta
     5701 ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc
     5761 gtggcaaaag tgagtatttc gggcttacca cgctttactg tcagcattgg tgtggcgcat
     5821 cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcga
     5881 gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccacggatgc gtctcgtgat
     5941 caacgactgc gcttagcatg gcgctcccag ttttcttgct tcgcctgcgc tgttttacgt
     6001 agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcagtgca atatgcctga
     6061 acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgcgat
     6121 ttatcatccc gtcctttgcc atgaataatc agcacgttac gcaaaccatc cgccagggct
     6181 tgttgaatga aactgaacat cattttgcgg cactcttcca ccggctggcg caaaagattc
     6241 aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc atgttgcaac
     6301 ccttcccgcc gaaactccag cggccgactt agcgggatga tgtcgagaaa tccagtggtg
     6361 aggaaattat caagttgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga
     6421 tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac
     6481 agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc gtgattcaga
     6541 cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca atactctact
     6601 tcatacctgt gagtatctgc gcaggcggag cattttatac tagcctgtta gcagtatcga
     6661 acagtttagc ccgagtttaa ttcagcacag gctccgatca ggatgattta ttcatagcag
     6721 gaaccaacaa aaatgcgata aataacagac cactgattag cagaaaggta tccgaaaatg
     6781 ccatagtttg cgcctccctc agcatcaatt gactcaataa tttggttgaa gccagtattt
     6841 ccgatgtctg gtcacttcct ccgcgattaa aaaacaaagc acttctggaa atgaagtcat
     6901 tcaccgtatg gggaacagaa accatttttt cacccattct cgaaaaatga aaattagttc
     6961 gattattgag aatagagcca cacagggcaa taccaatcgc gcccccaaga ttacgtgtta
     7021 aattaaatac accagaggct aacttcagcc gttcttttgg aattcccccc agcgttaatg
     7081 ttacgatggg ggccatcgca aactgctgcg aaatccctct aattgcctgt ggaaacagta
     7141 actcctgcca tccccattcg tgggttatag gggtaaaaag atacattgaa aacacaaatc
     7201 cacccagccc cgccatcaac agccatcgca gatttatttt tttacttaac cagaagtaaa
     7261 agggaacaga aaataactgg aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg
     7321 catttaatcc gcgaacccgc cccaaaaaca caggtattaa atatacagta gagaatattc
     7381 caacccctcc agaaaaagaa aaaaaacatc ccagtgtgaa atttttatcc ttaaaagcat
     7441 gaagatccat tatcggattg gatattgtaa gtgtacgcgc agcaaataaa ataaaactca
     7501 ccaatgccag aactgatgtc agaaaaatag tattatcatc cagccatccc cagcgcgctc
     7561 cttcttcaag ggtatattcc agacatccca gtgtcatggc caacaggata atactcggat
     7621 aatcagcaac tttcagtaaa gagagatcag gtttatcaaa att
//
LOCUS       DAJOTB010000200         7626 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJOTB010000200 DAJOTB010000000
VERSION     DAJOTB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.200"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            105..959
                     /gene="gatY"
                     /locus_tag="OHN28_004403"
     CDS             105..959
                     /gene="gatY"
                     /locus_tag="OHN28_004403"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCQ2299676.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            988..2250
                     /gene="gatZ"
                     /locus_tag="OHN28_004404"
     CDS             988..2250
                     /gene="gatZ"
                     /locus_tag="OHN28_004404"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCQ2299677.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2260..2712
                     /gene="gatA"
                     /locus_tag="OHN28_004405"
     CDS             2260..2712
                     /gene="gatA"
                     /locus_tag="OHN28_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCQ2299678.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            2743..3027
                     /gene="gatB"
                     /locus_tag="OHN28_004406"
     CDS             2743..3027
                     /gene="gatB"
                     /locus_tag="OHN28_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCQ2299679.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            3031..4386
                     /locus_tag="OHN28_004407"
     CDS             3031..4386
                     /locus_tag="OHN28_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCQ2299680.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRDFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGTIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4434..5474
                     /gene="gatD"
                     /locus_tag="OHN28_004408"
     CDS             4434..5474
                     /gene="gatD"
                     /locus_tag="OHN28_004408"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2299681.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5580..6353
                     /locus_tag="OHN28_004409"
     CDS             5580..6353
                     /locus_tag="OHN28_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2299682.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6435..7334)
                     /gene="yegS"
                     /locus_tag="OHN28_004410"
     CDS             complement(6435..7334)
                     /gene="yegS"
                     /locus_tag="OHN28_004410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCQ2299683.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1925 a         1693 c         1976 g         2032 t
ORIGIN      
        1 ctgattaatt ttcacattgt cgtttttgtg atcgttatct cgacatttga aaacaaataa
       61 tttcattata ttttgaaatc gaaaacaaat gacaggatgt gaaaatgtac gtggtatcga
      121 caaagcagat gctgaacaac gcgcagcgcg gcggttatgc ggttccggca ttcaatattc
      181 acaatctcga aacgatgcaa gtggtggtgg aaaccgctgc cagcatgcat gcgccggtca
      241 tcatcgccgg aacgcccggc acatttactc atgccggtac agaaaatctg atggcgctgg
      301 tcagcgcgat ggcgaagcaa tatcaccatc cactggcgat tcatctcgac catcacacaa
      361 aatttgacga tatcgctcag aaggttcgtt ctggcgtgcg ctcagtcatg attgacgcct
      421 cgcatttgcc ttttgcgcaa aatatttcac gggtcaaaga ggtggtggat ttttgccatc
      481 gctttgatgt cagcgtcgaa gcggagctgg ggcaacttgg cggccaggaa gatgatgtgc
      541 aagtcaatga agcagatgcg ttgtacacca accccgttca ggcgcgtgaa tttgccgagg
      601 caaccgggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg tatgccagag
      661 caccggcgct tgatttttct cgactggaga acattcgcca gtgggtgaat ttaccgctgg
      721 tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc aaactgggga
      781 tatgcaaaat caacgttgca acggagctga aaaatgcctt ctcgcaggcg ttaaaaaatt
      841 acctgaccga gcaccccgaa gcgaccgatc cccgtgatta tttgcagtcg gctaaatctg
      901 caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc agggcataac
      961 gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca taaagctggt
     1021 gaacatattg gcatatgttc agtctgttct gcacatccgt tggttatcga agcggcgctg
     1081 gcatttgatc gcaacagcac gcgtaaagtg ctgattgaag caacgtcaaa ccaggtcaat
     1141 caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt ttttgctatt
     1201 gccgataaag tcggatttgc tcgtgagcgt attattctcg gcggcgacca tctgggacca
     1261 aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga gctggtaaag
     1321 gcatatgttc gtgccggctt cagtaaaatt catcttgatg cgtcaatgtc ctgcgctgac
     1381 gattccatac cgttagcgcc agaaacggtt gctgaacgtg ccgctgtgct ttgtctggcg
     1441 gcggaaagtg tggcgactga ttgccagcgt gagcaactga actatgtcat tggcactgaa
     1501 gttccggtcc cgggcggtga ggccagcgcc attcagtcag tacacatcac ccaggttgaa
     1561 gatgccgcca atactttacg cacgcatcaa aaagcgttta ttgcccgcgg tctggcagag
     1621 gcgttaacac gcgtgattgc catcgtggtg cagcctggtg tggaatttga ccatagcaat
     1681 attattcatt atcaggcgca agaagctcag gcgttggcgc aatggataga aaaaaccaaa
     1741 atggtttatg aagcgcattc caccgattac cagactcagg cggcctatcg ggaattagtt
     1801 cgcgatcatt ttgcaatttt gaaagtcggt ccggcattaa cctttgcgtt acgcgaggcg
     1861 atatttgcgc tggcgcaaat tgagcaggaa cttatcgccc ccgaaaatag cagtggttgc
     1921 ctggcggtaa ttgaagaagt gatgctcgac gaaccgcagt actggaagaa atattatcgc
     1981 acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg tattcgttat
     2041 tactggccgc atagccggat taaaaatagc gtcgaaacga tgatggtgaa tctggaaggc
     2101 gtagacatcc cactgggcat gattagtcag tatcttccga aacaatttga acgcattcag
     2161 tccggggaat tatcagcaat accgcatcag cttattatgg ataaaattta tgatgttttg
     2221 cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct gtttgttcgt
     2281 ggcggaattt cttttgtcga tcgtagcgaa gttttaaccc atatcggtaa tgagatgctc
     2341 gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga agcagaattc
     2401 cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga ggcgattcat
     2461 gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttctttt tcagcaagcg
     2521 gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga aaatccgcag
     2581 cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga tatcgtcgag
     2641 acactaatca ctcttcctga accccagtta aaggaatact tcacaaagta tgttttagat
     2701 tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg caaaattatt
     2761 gtcgcttgcg gaggcgcggt agcgacctct acgatggcgg cggaagaaat taaagagttg
     2821 tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga aatagaaacc
     2881 tatatggatg gcgtgcattt gatatgtact accgccaaag ttgaacgcag ttttggcgat
     2941 attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc attacaaaat
     3001 aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg ttatattctc
     3061 gacctcggcc ctacggtgat gctgccgatt gtcatcatta ttttttctaa aatattaggc
     3121 atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt tgttggcatt
     3181 ggccttgtga ttggcttaat gctggattcc attggcccgg cggcgaaagc gatggcggaa
     3241 aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc acccatgacc
     3301 tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga ttctggttaa cgtggcgatg
     3361 ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg gcatatgacc
     3421 ttcaccggcg cgttgctgca tctggcaacc ggttcatgga tgatagggat ggcgggtgtg
     3481 gtaattcacg cggcgtttgt ttataagctc ggcgactggt ttgcccgcga tacccgtgat
     3541 ttctttgagc tggaaggcat tgctattccg cacggtacgt cggcgtatat ggggccaatt
     3601 gctgtgctgg tcgatgctat catcgagaaa atcccaggcg ttaaccgaat taaatttagc
     3661 gccgatgata ttcagcgcaa atttggccct tttggcgaac cggtcaccgt gggttttgtg
     3721 atggggctga ttatcggcat cctcgcgggt tacgatgtca aaggtgtatt gcagctggcg
     3781 gtaaaaacgg cggcagttat gctgctgatg ccacgggtga ttaaacccat catggatggt
     3841 ttaacgccca tcgctaagca ggcccgtagt cgtttacagg cgaagttcgg cggtcaggag
     3901 ttcctgattg gccttgatcc ggcgttgctg ctgggacata cggcggtggt atcggcaagc
     3961 ctgattttta ttccactcac cattttaatt gctgtttgtg tgccgggtaa tcaggtgctg
     4021 ccgtttggcg accttgccac catcggcttc tttgtggcga tggcggtcgc ggtgcatcgt
     4081 ggaaatctgt tccgcacctt aatctcgggt gtcatcatta tgagcatcac cctgtggatc
     4141 gcgacgcaaa ctattggttt gcacacccaa ctggcggcta atgctggcgc gttaaaagcc
     4201 gggggtatgg tggcttcaat ggatcagggc ggttctccca ttacctggtt actgattcag
     4261 gttttctccc cgcaaaatat tcccggcttc attattatcg gcacaattta tcttaccggt
     4321 attttcatga cctggcgtag agctcgaggc tttattaaac aagagaaagt cgttcttgca
     4381 gaataatttt tacctgaggg ggaggttatc ccccttaaca atcaggagtt tttatgaaat
     4441 cagtggtgaa tgatactgat ggtatcgtgc gcgtagcaga aagcgtcatt cctgaaatta
     4501 aacatcagga tgaggtgcgg gtaaaaattg ccagctcggg cttatgtggt tccgatttac
     4561 ccaggatatt taaaaatggt gcacattatt atccaataac gttaggccat gaatttagcg
     4621 gctatattga tgcggtggga tccggtgttg atgatttaca ccccggcgat gcggttgcct
     4681 gtgtgccgtt attaccctgt tttacttgtc cagagtgtct gaaagggttt tattcccagt
     4741 gtgcaaaata tgattttatt ggctcacggc gtgatggtgg atttgccgaa tatattgtcg
     4801 ttaagcgaaa caatgtcttt gcactacctg cggatatgcc tattgaggat ggcgctttta
     4861 ttgaaccgat taccgttggt ctgcatgcgt tccatttagc gcaaggatgt gagaataaaa
     4921 acgttattat tattggtgcc ggaaccattg gcctactggc tattcagtgc gctgtcgcgc
     4981 tgggagcaaa gagtgtgacg gctatcgaca ttagctcaga aaaactggca ctggcaaaat
     5041 ctttcggtgc gatgcaaaca tttaacagcc gtgaaatgag cgcgccgcaa atgcagggcg
     5101 ttttacgcga gctgcgcttt aatcagctta tcctcgagac ggctggcgta ccgcaaactg
     5161 tcgaactggc ggtagagatt gccggtcctc atgcccaact ggcgctggtg ggcacgttgc
     5221 atcaggatct gcatttaaca tctgcaacgt ttggcaaaat attgcgtaaa gagctgacgg
     5281 ttatcggcag ttggatgaac tactccagcc cttggccggg gcaggagtgg gaaacggcga
     5341 gccggttgct gacagaacgt aagttaagcc tggagccatt aatcgctcac cgtggaagct
     5401 ttgaaagctt tgcccaggcg gtgcgtgaca tcgctcgtaa tgctatgccg ggcaaagtgt
     5461 tgctcattcc ctgaaaccgc gggccagcgt gatgctggcc cggtattgtg caaaacagat
     5521 cattcaccaa tggttcccct tcgtttacac tagccacaat tgaatatggg taaatgacga
     5581 tgaattcatt cgagcgaagg aataagatca tccaattagt gaatgaacag ggaaccgtgc
     5641 ttgttcagga tctggcggga gtatttgctg cctcggaagc gacaatccgt gccgatttgc
     5701 gctttctcga acaaaaaggc gtggttacgc gctttcatgg cggtgcggcg aaaataatgt
     5761 ccggtaatag tgaaaccgag acccaggaag tcgggtttaa agagcgattt cagctcgcca
     5821 gcgcgccgaa aaacagaata gcgcaggcgg cagtcaaaat gatccacgaa gggatgaccg
     5881 ttattctcga cagcggaagt accacaatgc ttatcgccga aggattaatg accgccaaaa
     5941 atatcacggt gattaccaac agtctcccgg cggcgtttgc cctttccgaa aataaagaca
     6001 ttactctggt cgtctgcggt ggcacggtcc gccataaaac gcgatctatg catggatcta
     6061 ttgctgaacg ttccctgcag gatattaatg ccgatttaat gtttgtcggt gctgatggta
     6121 ttgatgcggt taatggtatt acgaccttta atgaaggtta ttccgttagc ggtgcgatgg
     6181 tgacagcggc gaataaagtg attgccgttc tcgattcatc gaaatttaat cgtcgcggtt
     6241 ttaatcaggt actgccaatc gaaaaaattg acattattat taccgatgat gctgtgtctg
     6301 aggtggataa actggcattg cagaagacac gggtaaaatt aattacggtg tagtattgtt
     6361 ctgtatgccg gataaggtgt ttacaccgca tccggcatta cggattctcc ctggttcaac
     6421 agagaatagt ttgattaacg caacaatgga caatctggtg gtaatcgaca acgcaacgcc
     6481 gccggaagta tttcaatatg gaagttttgc ccactcaacg gttcgccatc aagattaaaa
     6541 gtgatttcgt gtggggcttg tatatcaaac cacgacgaag caccttcgat aatattcggg
     6601 ttatcttcgt cagattttaa ggttgatacg agagtcggaa taatttcatc gccggtaaaa
     6661 atgcgcagtt gcagcaagcc atcgttaatt aatgcgttcg ggcacaattg ttgaccgcca
     6721 ccggcctgac gcccgttacc aataccaatg accagggcgt caccttgcca gtgaaagttt
     6781 tcaccgcgga tttcacaacg gtccggttgc agagtgtcca tgcgcattaa gccatgaatg
     6841 atgtaagaga cgccacccag cgcggctttt aatttttccg gcgtttctgt ggtaatacgc
     6901 gtcccaaacc cgcctgtcgc catattaata aaacaggttt gtttgttgac ctgcgccata
     6961 tctatcgcaa tggcgttacc ggcaattgcc agtttcagcg ccttatccag tgcctcagga
     7021 atccctacac tagtggcaaa atcattggca gttcctaatg gtaaaattcc cagcgcgggt
     7081 atgtcatccc cctcacattg aatcaatgcc gtagaaactt cattaatggt gccatcgcca
     7141 ccaccggcaa tcactgttgc gacgcctaac ttccgggcct cctctacaaa tcgtgcagca
     7201 tcgcctttct cccaggtgat ccgcacatgg atcgtcattc cttcctcacg caacagcata
     7261 attgcttcgc gcaagggtag attgtcagta cttttgccat taagaatcag taagctggcg
     7321 ggaaattctg ccatgatcgt atgtgccttt atgattggtc tgtagatagt gtagagcaga
     7381 aaagaaaagg tggagtcaga acaggatgaa agtcggagga taaaaagtca gttcaggtat
     7441 ggggattcag tgcttaccac caggacctta caatggggac agcgaatatt aacggtagcc
     7501 actgataaat tttaaaaaag agaatatacc taatattcaa ctaaacagtg gcatcttcaa
     7561 tatactattc aaaatatatt aaagtccccc atggggttac ccggaagggc ctctatgtct
     7621 gtaatt
//
LOCUS       DAJOTB010000201         7490 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJOTB010000201 DAJOTB010000000
VERSION     DAJOTB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.201"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            12..494
                     /locus_tag="OHN28_004411"
     CDS             12..494
                     /locus_tag="OHN28_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299684.1"
                     /translation="MKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKS
                     YDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPE
                     AVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK
                     "
     gene            572..1786
                     /locus_tag="OHN28_004412"
     CDS             572..1786
                     /locus_tag="OHN28_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299685.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1843..5175
                     /locus_tag="OHN28_004413"
     CDS             1843..5175
                     /locus_tag="OHN28_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HCQ2299686.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPFYLWQVCDNGDYQS
                     GRVLQSVGCLLPEGCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GITHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLAIGGAGMTTSFFANRALVQETSIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAP
                     DPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQV
                     LTPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAA
                     NLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAK
                     PARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYR
                     GSWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLP
                     GQVFLTGRSMKDAEEYGEDADE"
     gene            5168..6778
                     /gene="tssA"
                     /locus_tag="OHN28_004414"
     CDS             5168..6778
                     /gene="tssA"
                     /locus_tag="OHN28_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCQ2299687.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            6784..>7490
                     /locus_tag="OHN28_004415"
     CDS             6784..>7490
                     /locus_tag="OHN28_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCQ2299688.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKV"
BASE COUNT         1723 a         2025 c         2225 g         1517 t
ORIGIN      
        1 agattaaaat cgtgaaaacc tgtcaattga aaaaaaatga aagtgtgcag ttgctttccc
       61 ttagaaccat tgacggtgat acattatatc taaaattcag aaatgttatc accggggcct
      121 ttttagatac tccaactaaa agttatgatt tttttggaga tgtggttctt tctcagtgca
      181 taaatggttc tcttatcttt gcgctggaat atggttctcc atatataaaa ggttgtctgg
      241 taactggttg ggaaaatggg tttaaaggta agaactctcc tgaaggcttt tgtttcgcag
      301 agagaaatat tcccgaagcg gtatggtttg gaaaaaacaa tattttaatt atcatccgaa
      361 accaaatgga tgttgggaca tggaaaggga aatatattat ctatgataat gcgaaaaagt
      421 caggagaaag agcatactca tcagataatc taccatcaaa agatggttac ataatttttg
      481 cattaaataa ataaaatgac tctatacata actttaaatt tcacttggtt attaacttct
      541 ggtcgataat gcaaaataaa gggatgtact gatgccagtc cgacttaata ccattcccga
      601 cgcagaaccc tatcccgtac cgccagcccg aattaagtgg ctgacagctt tattactgat
      661 gctgtctgtc ggtgtggccc tgacttcatt attcgccagc gatgaactgg caaaaaacgg
      721 cccgtatttc tgggggctgg cctgtggtgt gcctgccttt atctggtcac tggttgcttc
      781 agttcgctgg ctgtttttta tcacgcagta cattcggact gatgcctgga ataagcgccg
      841 ggaagaggtg attttgcagg aaacgcgccg gggacgacgg gcgttgcaga tactcagttt
      901 ttccgtacaa accgcgctga atggtgattc cgttgcagaa actataacag catttctggc
      961 acgtcagcag gtgttgaata cctacgctga cttgcgcgga gaagatactg tgcgtcgcag
     1021 cgtcatccct gtattaacca ataaaccggt taccggacgt ctatccgaag ttttttcccg
     1081 tctgttcagc gatatcaggc tgcaactgac gcggctaccc gatgattttc atatcaacgt
     1141 actactggaa attgatgcgc cgctttcagg ggcctccgtg agaactatct ggcaggacga
     1201 atggcaaaaa gccggactcc cggaagcccg tctgttttca gccccggagc cggggctggc
     1261 cgccgtggac gactggctgg acaaccacat tcaggaaaag gccgtactgc tggtgatttc
     1321 cgtgcgtctg gaaccgaaga acccggaacg gacggcagaa tccgccaccg cgctgttgct
     1381 ggcaaaccgg ttgacgcaaa ccgcgctgac gccgctggcc ctgctgcatc gtcctgaatg
     1441 tatcactgat acagagatga tggcgtcagg cattgcacag gcgctggact ggatgccggt
     1501 gcagccagat gccatatccg gtatgtggac tgccgaactg gacagggaac agcgtgccgc
     1561 gttgttaagc ctgaaccagc cgtttgctca ggaagccctg atgtacgagc tggatgcctt
     1621 tctggggcgc tccggaccgg ctgcgccgtg gctgtccgtc gctgtggcaa cgctggcggc
     1681 aatacaatca caacaccccc aactgacgct cagcggcgtt cagggcgggc attattcatg
     1741 ggcgaccgtg gtgtcgccat tcgtttcccc tcaggaggca tcatagtggt tccgggatta
     1801 aaccgtctcc ggccccgttc ggtttcaggg cctgccatcg tcatgaccgt ggctgttgtg
     1861 gttgcgcttt gcacgtttat tcaccgtaaa ccggcattgc tgggcgttga accgggtagc
     1921 tcacagcaaa tgtactggat ggctggcgtg attgccgcag gcgtggtctt tctgattatc
     1981 atgggggttt atcatctgct gaaccgccac gctgccgtgc agaactttga actccgcaga
     2041 cagcttgccg ccggagatga ctatcgtgcg gtgacgacaa agaaagccga cagccgtcat
     2101 ccggcaatgg tggaactcgc cgccttcctg cgcgaccgct acggcccatt ctggcggcgt
     2161 aaaatccgcc tgctgctggt gaccggcgag cctgaacagg cagaggccat cgcgccgggg
     2221 ctgaccgggc aacactggct ggagggcgac cacacggtgc tgatatacgg cggcaggcca
     2281 tcagcggagc ctgatgtcgc gctactgacc gccttaaaaa aactgcgccg cagccgtccg
     2341 ctggacggca tcatctggcc gctgacagaa gaacagagcc gccagacagc gcaactcgac
     2401 aaaggctggc gcgaactgat aaacggcggt aagcgactcg gttttcaggc cccattctat
     2461 ttgtggcagg tctgtgacaa cggtgattat cagtccgggc gcgtcctgca aagcgtcggc
     2521 tgcctgctgc cggaaggctg taccccggaa caactggctg caatgctgga agcacagacc
     2581 ctaccgctga cggaacaggg catgtcgcag ctgctgaccg ataaccgcca tgattttctg
     2641 ctgcgtctgg cccataccct tgcagagcgg ggtatcaccc actggcagac cgtcctgaaa
     2701 ccgctgctgg caggcggcgc attttcttcc ctgcgcctgc gcggcctgat gttcagcccg
     2761 ccgcttgccg ccgtaccgga ggccgcacct catgcgtggc tgccgtcgcc ggtctgggca
     2821 ggcatcaccg gcgataacgc gcggggccgc gcggtgggct ttccgtggct gcgtaccgca
     2881 ctgatgtccg ctgtctgcgt gctggcgata gggggggccg gaatgacgac ctcgttcttc
     2941 gccaaccgcg cacttgttca ggaaaccagc atccagacgg cgcgggcgct cgatacccgc
     3001 ctgccgctgg cagaacaact ggtggcgctg cataccctgc agggcgaact ggaacgcctg
     3061 caataccgta tccgcgaagg tgcgccgtgg tatcagcgtt ttggccctga acgtaaccag
     3121 caactgctcg ccgccgcctt tcccggctat gcgcaggcgg caaaccggct ggtgcgcgac
     3181 gtggccgttg accatctgca acagcaactg aacgcctttg tcgccctgcc gcccaacagt
     3241 cctcagcgta ccgccaccgg tgaacaacgc tataagcagc ttaaggcatt gctgatgact
     3301 tcccgcccgg aaaaggccga cgctgccttt ttcagtacca cgctgatggc agacggcctg
     3361 cgctatgaaa atatcccgga aggcgtgcgg cagagcgtgt tgccgtcact gctgaccttc
     3421 tggatggcga acctgccgga acacccgcag tggaaaacct caccgccacc ggaactgacc
     3481 ggcgcggtgc gtaaaatcct gctgcgccag attggtgtgc gtaatgccga aaacaccctc
     3541 taccagaacg tgctgcaaca ggtgtcccgc aactacgccg atatgacgct ggcggacatg
     3601 accggggata ccctcactga atctcttttc agtacggaac agacggtgcc ggggatgttc
     3661 acccgtcagg catgggaagg acaggtcagg gaagctatcg agcaggtggt gacggcgcgg
     3721 cgcgaggaaa tcgactgggt actcagcgac cgccagcagg atacctctgc tgatatctcg
     3781 ccggatacgc tgcgtaaccg cctcacctca cgctacttta ccgactttgc cggaagctgg
     3841 ctggcgtttc tcaacagcat tcgctggaaa aaggaagact cgctctccgg cattctcgac
     3901 cagctgacgc tgatggccga tgcccgtcag tcgccactga ttgcactgac agacactatt
     3961 gcgtggcagg tggcaacagg cagggaaaat cgtggcctgt cagactcgct ggcgaaatcg
     4021 gcacaggaac tgtttaacgg caaggagaaa acgccgcagc aatcccgtga aggcgacgtg
     4081 cctgccgggc cgctggataa aaccttcgcg ccgctgctgc ggttgctggg cgataagtca
     4141 ggaggcggtg gtggtgatac gcaactcaac ctgcaaacgt atctgacccg cgtcacccgc
     4201 gtacgcctca aactgcaaca ggtgaccaac gcccccgacc cgcaggagat gacccagcaa
     4261 ctggcgcaga cggttttaca gggcaaaacc gttgacctca ccgacacccg cgactacggg
     4321 cggttaatcg ccgccagtct gggcgaagaa tggagtggct tcggtcaggc gctgttcgtc
     4381 cgcccggtgg aacagtcgtg gcggcaggtg ctgacgcccg cagcggacag cctgaaccgc
     4441 cagtggcaac gggcgattgt cagtcactgg aatcaggact tcgctggccg ctatccgttc
     4501 aaagcctcac agaacgatgc ctctcttccc ttgctggcgc agtacctgcg cgatgacgga
     4561 cgcatcaacc agtttatcgc cgccaacctt tccggtgttc tgaaacgtga aggccgctac
     4621 tgggtggctg acgccatgaa cacgcagggg ctgacggtca acccggactt tatccgcgcc
     4681 ctgaaccgcc tgcgcgatgt ggccgatacc gcctttgcca gcggcgatgc cggaatacat
     4741 ttcgaactgc gggcaaaacc ggcgcgtgac gtgatgaaaa cgcatctggt gattgacggg
     4801 caggaactgg agtatttcaa ccagaaagaa cgctggcagc gttttaactg gccggatgaa
     4861 cagtggcaac ccggcgcatc gctgagctgg accagcacac aggcgatgga acgcatactg
     4921 gcggattacc ggggaagctg gagccttatt cgcctgctgg aacgggcgca ggtaacaccg
     4981 gtcgacagca gcacctttaa agtggtgtgg aaagcgcagg acggcctgcc actgaattac
     5041 ctgctgcggg ttgaacaggg taaagggccg ctggcgctgc tggaactgaa aaacttccgc
     5101 ctgccgggac aggtatttct gaccggcagg agtatgaagg atgcggaaga gtatggggaa
     5161 gacgccgatg agtgaactgt tgcaaaagct gacccgtagc tgttttgccg accgggatgc
     5221 actggatgtg gcgcgaacac aggcggcgct gtggcagaca tggctgctgc cggtgacggc
     5281 agacacgccg gtcggggaag accccggcta ccacgacgat ttcctgcgta tccgcgacga
     5341 aatgaacaaa ctgtccggtg ccgataccgg ccttatctgc cagcttgccg aatccctgct
     5401 gctgacgcag gccaaagacg tgcgcatcgc cacttactac atctgggcgc gactgcaccg
     5461 cgacggtgag cgcggactgg ctgaaggact ggcgctgtta gccgggctgg tggaacgctt
     5521 cggtacgcaa ctgctgccct cgcgaccggc aagccgaaaa atggcgctgg agtggctggc
     5581 gggggaaaag atgctcgaca gccttgcccg ctacccggag gtggcaaaag aggattttgc
     5641 gaacattgtt gccgctctta atcaactgtc ggtttccttc actgcatggc ccgaagacca
     5701 gcattccccc tccctgatgc cgctgattaa cgcgctggaa tcacgtctgg cgcagtcggg
     5761 cgggatgaac gcggtggtgc cgcaaaacag cagcggcgtt ccggcaccgt cttccccggt
     5821 ggatgcgccg caggtacaga ccatcacctc cgggcgcgac ctactcgacc aggcgaaagt
     5881 gctggcccgt tatctgaacg aacagccgca gggctggctt tcagcacacc ggctgatgaa
     5941 aaccctgcgc tgggacacgg tgcatgaact gccgccggac gtggacggca aaacccggct
     6001 ggccccgccg cgcaccgaat cacgcaacca gcttaagcgg ctgtatgccc agcagaactg
     6061 gacggaactg ctggagcagg ccgacctgat gttcagcacc ggtgtcagcc atttctggct
     6121 ggatatccag tggtatctgc atcaggcgct ggcgaaggcg ggcgcaccgt gggacagatg
     6181 gacagcggtt atccgtcagg acctggcgct gctgctggag cgcctgccgg ggctggaaaa
     6241 tctggcatgg aacgacggca cgccgtttgc cgatgaggtg acgcgcaact ggatagcgca
     6301 gcaggtcatg atgcgggaag acggcgcatg gcttgcgggg aaagccgctg tgccgacaga
     6361 cgacgccaca aatgacgtac tggcgctgga gccggaagca ctggagatgg ctgacagtca
     6421 gggcgtggaa gcggcgctgg gctggataca gacccgtccg ggcatcacca cggcacgaca
     6481 gcgcctgtta ttgcgactgc tgatggcacg ggtggcggaa cagtacggta aaaacgagat
     6541 ggcgctcctg ctgctggaag aactggatac cgccgcacag ggaataacgc tgacacagtg
     6601 ggagccagag ctgctgtttg aagtaaaagc ccgacaactg aaactgctgc gtctgcgggc
     6661 gcaccgctat gcggataaag ccctgctgaa ccgaaaaatg gaaatcctgc tgggaacgct
     6721 ggtggcaatc gatccggtac gggcagcggt attgtgtgat acgcaacata aggactgatg
     6781 gcaatgggaa caggctactt tctggtcagg ggggataaga ccacctgtgg tggaaaaatt
     6841 attgaagggg cggacgacca tacgatcatg ggtatcccgc aggcacggga tatggaccgg
     6901 gtcacctgcg gcagatatcc gggaatgttt attattgtgg gcggcgttcc tgaaacagat
     6961 attcacggca ggctgatggc ggggacgctg gacagtcaga gcagttgccc gtgtaaagca
     7021 cggtttattg cgtcaatgat ggatgatacg tatgagacgg atgacgggga aagtgaagcg
     7081 gaacaacatg cgcagtcggc gaaaaaggat ctgacttctg gttctgattc atcctctgat
     7141 gatgtaaaac tggattacag aattaaatta tcaggtaata agatactgac gccactgaat
     7201 atacctgatt ataaagaaat gataaacggt ggtagcacga agaatacaga aaaaatagat
     7261 tttaccatca caaataaagg cgatgaggct gaaggacttt ccctggaagt gcttgatggc
     7321 aatgaagtga tttatagtga acggcagacc ggtaaatatt ttactaaggg agagcatgcc
     7381 tggcaatggg atggctacag taatcaaggc atactggata caactaagct aaaaagtaaa
     7441 tcactactcg taagactgat tgctttatgt ggtgatatga tgataaaagt
//
LOCUS       DAJOTB010000202         7480 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJOTB010000202 DAJOTB010000000
VERSION     DAJOTB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.202"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(202..948)
                     /gene="dsbG"
                     /locus_tag="OHN28_004416"
     CDS             complement(202..948)
                     /gene="dsbG"
                     /locus_tag="OHN28_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCQ2299689.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
     gene            1320..1883
                     /gene="ahpC"
                     /locus_tag="OHN28_004417"
     CDS             1320..1883
                     /gene="ahpC"
                     /locus_tag="OHN28_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCQ2299690.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            2054..3619
                     /gene="ahpF"
                     /locus_tag="OHN28_004418"
     CDS             2054..3619
                     /gene="ahpF"
                     /locus_tag="OHN28_004418"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCQ2299691.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3740..4168)
                     /gene="uspG"
                     /locus_tag="OHN28_004419"
     CDS             complement(3740..4168)
                     /gene="uspG"
                     /locus_tag="OHN28_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCQ2299692.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(4524..4934)
                     /gene="rnk"
                     /locus_tag="OHN28_004420"
     CDS             complement(4524..4934)
                     /gene="rnk"
                     /locus_tag="OHN28_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCQ2299693.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(5164..5970)
                     /gene="rna"
                     /locus_tag="OHN28_004421"
     CDS             complement(5164..5970)
                     /gene="rna"
                     /locus_tag="OHN28_004421"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCQ2299694.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(6084..>7480)
                     /gene="citT"
                     /locus_tag="OHN28_004422"
     CDS             complement(6084..>7480)
                     /gene="citT"
                     /locus_tag="OHN28_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCQ2299695.1"
                     /translation="IPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICV
                     IGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRR
                     IALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKSFPN
                     DPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCFL
                     PVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLG
                     LWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTR
                     SGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKG
                     IPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYIS
                     MLLLVGWPILAMWN"
BASE COUNT         1954 a         1825 c         1864 g         1837 t
ORIGIN      
        1 atactccgtt gtattgtctc ttccataaat cattcacttg atcaataatg ttgttgtatt
       61 tatatgtatt atgtttttct gcttaacttt ggattaagat tttctaaaat agcatagctg
      121 tcaggtgggt tacgagcaat tcatgcgttt ttaggatggt tgaaaagcaa tgatgtattt
      181 caaccatcca tgaattatct tttatttatt cctcataatg atattaagcg ttttctgatc
      241 gggtaatccg acggcttgtt gcagggtatt ttccttactc atgtaataga tagctggtgt
      301 gacatttgcc cccagatcgt ccatgagttt ctcgttgtcg cttaacactt tcatttgctc
      361 tgtgcttacg tttgcaggca cgcttagctt aagcttgcca ccagaggctt catattgttg
      421 ccaggttttt gcgggatctt tggaggcaag aattgccgct gctgtcgccg ggctttctgg
      481 cttgataacc ccaaccaaca atgttctcaa ttgcactttg ccagaatcta cccacggacg
      541 agcctgctgc cagaactgtt tacaatatgg gcagaacgga tcggcgaaga cgtagacaat
      601 gaccggcgcg tcttttttac cgtcgaggag ccagtgggat tgttccatcc gttgccacat
      661 ttcacgtccg gcgggtgcgt aaatttcttt ttcgataagc gtgttactca ggttttcacc
      721 tttttcgttg tacatgtaac cagagatagc gtgcttacca tctggagtca ggtagatggt
      781 gacgcccata tcctgatact taccgagata acctttcatt cctccagggg catcgaacgt
      841 tttgatgatt gtaataccct gtttttcaat cgcttttact ggggcaggaa gttcctctgc
      901 gaaggcgatt gcaggaagca gggccagtaa aagtatcttt tttaacatta atttgtcctt
      961 ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc actgtcatag gtaagagctt
     1021 agatcaggtg attgcccttt gtttatgagg gtgttgtaat ccatgtcgtt gttgcatttg
     1081 taagggcaac acctcagcct gcaggcaggt gctgaagata ccaaagggta gttcagatta
     1141 cacggtcacc tggaaagggg gccattttac tttttatcgc cgctggcggt gcaaagttca
     1201 caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga tttgataatg gaaacgcatt
     1261 agccgaatcg gcaaaaattg gttaccttac atctcatcga aaacacggag gaagtataga
     1321 tgtccttgat taacaccaaa attaaacctt ttaaaaacca ggcattcaaa aacggcgaat
     1381 tcatcgaaat caccgaaaaa gataccgaag gccgctggag cgtcttcttc ttctacccgg
     1441 ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt tgctgaccat tacgaagaac
     1501 tgcagaaact gggcgtagac gtatacgcag tatctaccga tactcacttc acccacaaag
     1561 catggcacag cagctctgaa accatcgcta aaatcaaata tgcgatgatc ggcgacccga
     1621 ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga tgaaggtctg gctgaccgtg
     1681 cgaccttcgt tgttgacccg cagggtatca tccaggcaat cgaagttacc gctgaaggca
     1741 ttggccgtga cgcgtctgac ctgctgcgta aaatcaaagc agcacagtac gtagcttctc
     1801 acccaggtga agtttgcccg gctaaatgga aagaaggtga agcaactctg gctccgtctc
     1861 tggacctggt tggtaaaatc taaatttcct tcgtctttcg cgctatcaac tccggcgcga
     1921 gatacttcgg aaattcacct aattcttcgg gtgctgcggc acccgatttt cttccccgca
     1981 ccatgatgca agctgcatcc agctagccgc agaggccgct tgcatgatga tgtttaaagc
     2041 ccaggagata aacatgctcg acacaaatat gaaaactcaa ctcaaggctt accttgagaa
     2101 attgaccaag cctgttgagt taattgccac gctggatgac agcgctaaat cggcagaaat
     2161 caaggaactg ttggctgaaa tcgcagaact gtcagacaaa gtcaccttta aagaagataa
     2221 cagcttgccg gtgcgtaagc cgtctttcct gattaccaac cctggttcca accaggggcc
     2281 acgttttgca ggttccccgc tgggccacga gttcacctcg ctggtactgg cgttgttgtg
     2341 gaccggtggt catccgtcga aagaagcgca gtctctgctg gagcagattc gccatattga
     2401 cggtgatttt gaattcgaaa cctattattc gctctcttgc cacaactgcc cggacgtggt
     2461 gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc aagcacactg caattgacgg
     2521 cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg ggcgttccgg cagtgttcgt
     2581 aaacgggaaa gagtttggtc agggccgcat gacgttgact gaaatcgttg ccaaaattga
     2641 tactggtgcg gaaaaacgtg cggcagaaga gctgaacaag cgtgatgctt atgacgtatt
     2701 aatcgttggt tccggccctg cgggtgcagc ggcagcaatt tactccgcac gtaaaggcat
     2761 ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc ctcgataccg ttgatatcga
     2821 aaactacatt tctgtaccga agaccgaagg ccagaaactg gcaggtgcgc tgaaagttca
     2881 tgttgatgaa tacgacgttg atgtgatcga cagccagagc gcgagcaaac tgatcccggc
     2941 agcggttgaa ggcggcctgc atcagattga aacagcttct ggcgcggtac tgaaagcacg
     3001 cagcattatc gtggcgactg gtgcaaaatg gcgcaacatg aacgttcctg gcgaagatca
     3061 gtatcgcacc aaaggcgtga cctactgccc gcactgcgac ggcccgctgt ttaaaggcaa
     3121 acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa gcggcaattg acctggcggg
     3181 tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa atgaaagccg accaggttct
     3241 gcaggacaaa ctacgcagcc tgaaaaacgt cgacattatt ctgaatgcgc aaaccacgga
     3301 agtgaaaggc gacggtagca aagtcgtagg tctggaatat cgcgatcgtg tcagcggcga
     3361 tattcacaac atcgaactgg ccggtatttt cgtccagatt ggtctgctgc cgaacaccaa
     3421 ctggctggaa ggcgcagtcg aacgtaatcg catgggcgag attatcattg atgcgaaatg
     3481 cgaaaccaac gtcaaaggcg tgttcgcagc gggtgactgt acgacggttc cgtacaagca
     3541 gatcatcatc gccactggcg aaggtgccaa agcctctctg agtgcttttg actacctgat
     3601 tcgcaccaaa actgcataag aagaagtaag attcacctgt aattgcttag ccgccggggt
     3661 caaacctggc ggctttttta tggcattaaa aagccctgca gggatggctc cgggcagggc
     3721 gggagactta ttctggcaat taacgcacaa ccagcaccgg caaattggcg tggcggatta
     3781 cgctcgaggc gttagaaccc aacagatgag ttgaaattga tgggttacgc gaaccaatca
     3841 ctactacatc tgcatcaagt tctttcgcta actcgttaac ttcatcccgc acactaccaa
     3901 aacggacatg ttgcttaatg cgggaaggat cgatggtgaa gtggctgacc atcgtttgca
     3961 aacgttcttc tgcttcatgt tgcagatgct cttcaaaacg acgtacatca gcggcaaaac
     4021 ggtgcaggct caggctggct gaccccggta gtacgtgaag tagatgaata actccgtcat
     4081 cctgggcgag gaattcagcg tggcgaatcg ctttgtcgct caattccatt tcgaaaacat
     4141 ccactggcat gatgattgtt ttgtacataa gcattctctc cttgtttttg catgtataca
     4201 taacagcaca ttattggtta tgaatctata atttgcgagg ctgatttgat ctgaatttgc
     4261 tattgagctg gaaaataaat aaaaccaggc gctaccagcg ttgttcaagg tatagcactg
     4321 cagcaacaat aattgctgct atgaatatat aaaatgcagt gataccgaga gcttcgctca
     4381 tatgaggttc cgcagatgaa taaccagttc agattagaat gctgcgctta agagcaaaat
     4441 gataaatctg gttgatattt gagcggaaag aggatgcgag tgcatcctct gggcaaagcg
     4501 agttatcgct tgtgcagatg ggattaaagc aggtagtcgc cagcagcttc tggctggtat
     4561 tcgagttcca gcacttcaag gtgggttgca acgccgcccg gaagttccca gtgaatggaa
     4621 tcgccaacgc gcagccccag cagtgcggca cctaccggag ccataacgga aagctgagta
     4681 ttgctatcgg tcatttttgc cggatacacc agcgtgcgca cacgcacttc gccatcgcta
     4741 agattgcgga atttaacccg gctgttcatt gtcaccacgt cgtgtggcat ctcttctggc
     4801 gaacacattt gggcgcgatc caactctgcg tttaacgcgt cggcgattgg caaaccagca
     4861 taggcgggtt gctccagcag aatatcgatg cgttcggcat ccaggtcgtt aatgatgata
     4921 gttggtctgg acatttttac tccatgtcgt cggtactgcg agtgtcgcag ataaacatac
     4981 ccaaaagaaa accctcaccg tcaggcggcg agggtttaac tcacatgatg atactgactg
     5041 ttgctcactc tttgaagtga tttgcgtcac attcagggaa ttcctcaatg tagcgcatta
     5101 tgtataaatc ttaatcgtca tggtttatgg aagacgaatt agcgtgtttt gtaaatcaga
     5161 ggattaataa cctgctttat caatcacaaa ggttttgcca cagttgcctg ggtgtggttg
     5221 tggcaagaat gagttggcag aaagcggagc gttgatggcg tcagctttga tcgagatctg
     5281 aatttcagtc aaatacgcag ggttaccctg gcacgttagc ttaactgctt tcacgttctc
     5341 ttttccccag cttttggcaa aggcggcatc aaagtcacgg cggctcactg ttttaccgta
     5401 gttatccgca aggaattttc cggcttcgct ctctttaatt tcttgattca ggcgtaccat
     5461 cgtgccgaag tatgcgtctg gatcaaaacc aaagcaggca ccgtgtttgg catattcgta
     5521 gcgttccagg caggaacgtc cgccagctcc tggcatgact tcacttagtt tagctgccgt
     5581 ttccagcgat aacccggttt ccggcgatga acacatccgg ctggcgcgtg cttctggcag
     5641 attcgggatt gggcgagtag cgcaaccgaa gcgcatccag cggcgttcat caacaccacg
     5701 ggcagcaacc gatttaggca atcctggcca cagaccatgt acggtcaaaa aatcagcttt
     5761 gttggtcgtt tcggtttgca ggcgacattc atctcgttcg ttacgatttc gatcgtgttg
     5821 actctggcaa aatccggttt gccaggagag ggccaggaca tagcgatcaa aatcgccata
     5881 ctgttttgcc tgcaacgcca cggcgttggc agaagagaag ggaagcagag aaaccgcgag
     5941 caacgcggcg ttacgccaga atgctttcat aatgggtgtg gaactcatac atacactgaa
     6001 tattatctat taaatcataa aaaagcccgc caggtgggct tacctggcgg gcgtgatgat
     6061 ttattcagcg tttggcgaac gtattagttc cacatggcga gaatcggcca gccaaccaac
     6121 agcagcatag agatgtaaat caccccgaag attgcgccaa gacgccagta atcttttgat
     6181 ttcacatagc cacagccgta aataatcacc ccaggaccgg ttgcataagg cgtcagacag
     6241 cccatgatac cgatagacag caccagcagg atacacagtt gttccattgg tacgcccgga
     6301 atccctttac cgacggccag aataaccggc agcatggttg cggtgtgcgc agacaggctg
     6361 gcaaacaggt agtgtgcaaa gtagaacacc agaaccagta caatcaccgt tgcgtttggt
     6421 gagaagcctt ccaggtgtgt actcatcgtg ttggcgaacc agtcgataaa gccagaacgg
     6481 gtcaggccat tcgccataac aaccagcgtt gccaggttga ccagcgtgtt ccatgcgctg
     6541 ttatagcgag taatgtcttt ccacggcaca acgtgcagcg ccagcatcag cgaaactgcc
     6601 agcagaccta ccgcagtagc attaatgact tcactgccaa atacccacaa acctaagctg
     6661 agcaatacaa ggccaatcag cgtccactct ctgcgcgtca gcgcacccat ggtttttagt
     6721 tcatcacccg cccaggttgc cacttcttca ctgtgtgtga tttccggttt gtacagcacg
     6781 taggaaagcc acggcgcaat gataagcaag ataaccccaa ccggcaggaa gcagaggaac
     6841 cactgcaacc agctaatctg gataccggca attttgctga cgaactccag acccagcacg
     6901 tttggtgccg caccggtgac aaacatggac gaactcagac tggtactaat gaccatcatc
     6961 cacatcaaat agccgccaat acgacgcgcg gacggatcgt tcgggaatga tttaaacagc
     7021 ggcggcaggt ttttaatgac cggaaaaacc gtacccccgg tacgcgcggt gttggacggt
     7081 gtaaacggtg ccagcagaat gtcgataatg acaatcgcat aacccaacgt cagcgtgcgt
     7141 ttccccatga atttcaccag gaaaagggca atacgacggc ctaacccgga aacttcatac
     7201 cctaatgcaa aaataaatgc gccaaatacc agccataccg tggtgctgga aaaaccagcc
     7261 agaccccatt tcagcgcctg tttttgcgca ttaaacgctg ggtcagctaa ttctttggca
     7321 tcaaagagca ggtaattact gccaataacg caaatagtaa ccgcaataaa actgatcgcc
     7381 gttgccggaa ttggctcgag gatcatgccg acaatcattg ccacaaacac tgcaaagtaa
     7441 tgccacgcct gcggcggcat accgtcaggt acagggataa
//
LOCUS       DAJOTB010000203         7394 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJOTB010000203 DAJOTB010000000
VERSION     DAJOTB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.203"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            153..1139
                     /locus_tag="OHN28_004423"
     CDS             153..1139
                     /locus_tag="OHN28_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCQ2299696.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETRDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            1154..1495
                     /locus_tag="OHN28_004424"
     CDS             1154..1495
                     /locus_tag="OHN28_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCQ2299697.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPSMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            1500..3146
                     /gene="fliF"
                     /locus_tag="OHN28_004425"
     CDS             1500..3146
                     /gene="fliF"
                     /locus_tag="OHN28_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCQ2299698.1"
                     /translation="MNAQIKKLTQAFPAFRLHLVDNKRWVLMAGVGLAVAVTAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            3124..4134
                     /locus_tag="OHN28_004426"
     CDS             3124..4134
                     /locus_tag="OHN28_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCQ2299699.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINHFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            4138..4848
                     /locus_tag="OHN28_004427"
     CDS             4138..4848
                     /locus_tag="OHN28_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HCQ2299700.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            4841..6181
                     /gene="fliI"
                     /locus_tag="OHN28_004428"
     CDS             4841..6181
                     /gene="fliI"
                     /locus_tag="OHN28_004428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HCQ2299701.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTRLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            6184..6618
                     /gene="fliJ"
                     /locus_tag="OHN28_004429"
     CDS             6184..6618
                     /gene="fliJ"
                     /locus_tag="OHN28_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HCQ2299702.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            6621..7016
                     /locus_tag="OHN28_004430"
     CDS             6621..7016
                     /locus_tag="OHN28_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HCQ2299703.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGEQLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRGHF"
     gene            complement(7036..7383)
                     /locus_tag="OHN28_004431"
     CDS             complement(7036..7383)
                     /locus_tag="OHN28_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299704.1"
                     /translation="MKKVYFVHRDKNAIERQSDGVEFCFIPEFNDGRIYFYCHEYDIF
                     WRSIKDAGDYAWCCNFHLKGIIRPATLIEISNSDLISYIDSIKEYEIENSKLININYI
                     HLNYDFLNIHQNT"
BASE COUNT         1795 a         1980 c         2128 g         1491 t
ORIGIN      
        1 tgcggcgtaa acgccttatc cggcctacat tgctaactag ttgtttataa acaaataata
       61 tttttattat cgtttaattc gaacttgtta atcccaccgg tgatacgctt cagaacatcc
      121 tgttttctgt cgctacttat atcgttcacc ctatgtctga acttattgca actgccgctt
      181 ccagcattaa cgcttttact ctggcaaagc gtgtcgccgc ctttaacgta ccggtgctca
      241 ttcagggcga aaccggcgcg gggaaagagt gcgtggcgaa atatattcac accgtagcct
      301 ttggtgaaaa tgataacgcg ccctatatcg gcgtgaactg tgcggcgatc ccggaaaata
      361 tgctggaagc gaccttattt ggctacgaca aaggcgcatt taccggcgca attgccagcg
      421 tgccaggaaa aatggaactg gctaataacg gcaccttatt gctcgatgaa attggcgata
      481 tgccgctggc attacaggcc aaaatattac gcgtattgca ggaacagcag gttgagcgat
      541 taggcagcaa tagacaaatc aaactcaatt ttcgcctgat tgcctgcacc aataaaaatc
      601 tggaacagga cgtggctgct ggacgttttc gtgaagatct ttattatcgt ctggcggtta
      661 ttcctataac catgccgcca ctgcgtgaac gtctgaacga tattattccg ctggcagagt
      721 cgtttattaa aaaatatgcc acggtgctgg tgaaaaatat caccctgtcc gaatccacac
      781 gccgggcgct acttaattac cgctggcccg gaaatgttcg ccaactggaa aacgccattc
      841 agagaggaat gatcttaaac cgcgacggcg tgatttaccc ggatgcctta ggcctgccgg
      901 aaactgacat tgccgaccac agcgaactgc aatggcctgt tcagcccgcc gtccacattg
      961 ccgaaacccg cgatttaggc cagcacggac gaagtgcgca gtatcaatat atcgccgacc
     1021 tgatgcgtaa atatcagggc aaccgcagca aaatcgccga cctgttaggc attaccccgc
     1081 gcgcactgcg ctatcggctg gcctccatgc gcaagcaagg tatcgaagtt ttctcctgac
     1141 ccggaagtta aaaatgagca ttaccacaat caacagcacc atgcaggcgc aaatcatgca
     1201 ggacgtgcag cggatgcagg ccaacgccca ggcgccggtg ctaccctcca tgaccttcag
     1261 ttccacagac ccggacgtgt cgtttaaccg cattatgtac ggcgcgcttg gtcacgttga
     1321 tcagttccag caggtggcgg agcagcagca aacggctgtc gacacgggca aaagtgatga
     1381 cctggcaggt gcgatgatcg ccagccagca ggcgtcgctc tctttctccg cgctggtgca
     1441 ggtgcgcaat aaaatcgcca ccgggtttaa cgacctgatg agtatgtcga tctaacccta
     1501 tgaatgcaca aataaagaag ttaacccagg cgtttccggc gtttcgactg catctcgtag
     1561 ataacaaacg ctgggtgttg atggcgggag tggggcttgc cgtggcggtg acggcgatta
     1621 tcgtcagcgt gctgtggacc ggtaatcgcg gctacgtttc gctctacgga cgccaggaaa
     1681 atctgcccgt gtcgcagatt gtcacggtgc tggacggcga aaagctcacc taccgcatcg
     1741 acccgcagag cgggcagatt ctggtgccgg aagatgcact gtcgaaaacg cgaatgaccc
     1801 ttgccgcgaa aggcgtgcag gcgatattgc ccagcggcta cgagctgatg gataaagacg
     1861 aagtgctcgg cagcagtcag ttcgtgcaga acgtgcgcta taaacgcagt ctcgaagggg
     1921 agctggcgca gagcatcatg tcgctggacg cggtggaaag cgcgcgggtg catctggcgc
     1981 tcaacgaaga aagctcgttt gtggtcagcg atgagccgca aaacagcgcc tcggtggtgg
     2041 tgcgcttgca ttacggcgcg aagctgaata tggaccaggt gaacgccatt gtgcatctgg
     2101 tgtccggcag tattccgggg ctacaggcca gcaaagttag cgtcgtcgat caggcgggaa
     2161 atctgctgac cgacggcatt ggcgcaggcg aggcggtttc tgccgcgacc cgtaaacgcg
     2221 atcagatcct caaagatatt caggacaaaa cccgtgccag cgtggcgaac gtgctggatt
     2281 cgctggttgg cacggggaat taccgcgtca gtgtgatgcc ggatctcgac ctcagcacca
     2341 tcgacgaaac ccaggaacac tacggtgacg cgccgaaaat caaccgcgaa gagagcgtgc
     2401 tggacagcga caccaatcag gtggcgatgg gcgtgcctgg ttcgctcagt aaccgtccgc
     2461 cgattgcagc gaatcagatg accaacggta cggaagaaaa ccgctcgccg gaagcgcttt
     2521 ccaaacacag cgaaagtaag cgcgactact cttacgaccg cagcgtccag catattcagc
     2581 atcccggctt cgcggtgaaa cgcctcaacg tggcggtggt actcaatcaa aacgctccgg
     2641 cgctgaaaaa ctggaagccg gagcaaacca cgcaactgac cgcgttgctg aacaatgccg
     2701 ccgggatcga cgcccagcgt ggtgacaatc tctccttatc gttactcaac tttgtaccgc
     2761 aggcggtgcc tgtcgaaccg gttattccac tgtggaaaga tgacagcgta ctggcctggg
     2821 tgcggctgat tggctgcggc ctgctggcgc tgttgctgct gttcttcgtg gtgcgtccgt
     2881 tgatgaaacg gctgacggcg gtacgtgcgc cggttatcac accagaaccg gaagccgtca
     2941 gcgaaccgtg gattgccatg ccggaagagg agcgcaaaaa cgtcgacctg ccgtcgctgc
     3001 ccggcgatga cagcctgccg tcacaaagtt ctggcctcga agtgaaactc gagttcctgc
     3061 aaaaactggc catgagcgac accgatcgcg tagccgaagt tctcagacaa tggatcacca
     3121 gcaatgagcg aattgacaac aaataagagc aacagctacc tggaacaggc ggcgatcctg
     3181 ctgctgtgtc tgggcgaaga ggcagcggcg acggtgatgc aaaaactcag ccgcgaagaa
     3241 gtggtgcgcc tgagcgaaaa catggcgcga ctttccggcg tcaaaaccag catggcgcgt
     3301 aaagtgatca accacttctt tgacgagttc cgcgaacaga gcgggatcaa cggcgcatcg
     3361 cgatccatgt tgcagggcat tcttaacaag gcgttgggta ccgagatcgc cagcagcgtg
     3421 atcaacggca tctatggcga tgagattcgc tcgcggatgg cgcgcctgca atgggtggaa
     3481 ccacgccagt tggcgatgct gatttccgaa gaacatctgc aattgcaggc ggtgttcctc
     3541 gcgtttctga cgccggaaat ctcggcagcg gtgctctctt atctcaacga atcggtgcaa
     3601 aacgaaattc tctaccgggt ggcgaaactc aacgacgtca accgcgacgt ggtggatgag
     3661 ctggaccgcc tgattgaacg tggcctgtcg gtactttcgg aacatggctc gaaggtaaaa
     3721 ggcatcaaac aggcggcgga tatcgtcaac cgcttccagg gcaatcagca ggtgatcctc
     3781 gaccagatgc gcgaacgcga cgaagaggtg ttggggcaga tccaggatga gatgtacgac
     3841 ttctttatcc tcagccgcca gaacgaagaa gtgcgtcggc gtctgcttga tgaagtgccg
     3901 atggaagact gggcggtggc gctgaaaggc accgaagccc tgctgcgccg ctcgatttac
     3961 gcggtaatgc caaaacgtca ggcgcagcaa ctggaagcga tcaccgcgcg tctgggtccg
     4021 gtgccggtta gccgtatcga acagatccgc cgcgaaatca tgggcatcgc ccgtgagctg
     4081 gaagaagcgg gcgaaattca gctacagctc tttgcggaac ggacggcgga gtaattcatg
     4141 accattgaaa cactgcgcgg gcgctatcgc ctgcaccgct tcccgccgcg ccagcgtgcg
     4201 gtgaccggag aaccgcttac tgccagtatc agcgccgccg attaccagcg tcagttgatg
     4261 gacggttttc aggaagggct gcaaaaaggg tttgctcagg gaatgaccga aggtcaggag
     4321 cagggcttta gcgaaggtca tcaacaaggt tttgccgagg ggcggcgtca gggctacacc
     4381 gaaggcagcc tcgccgggca gcaggaagga cgcaaacagt ttgttgaggc ggcgcagccg
     4441 ctggaagcca tcaccggtaa agtgaacgac ttcctcgcgc atattgaacg taaacagcgg
     4501 gaagatttgc tgcaactggt ggagaaagtc acccgtcagg tgatccgctg cgaactggcg
     4561 ctgcaaccga cgcagttact ggcgctggtg gaagaggcgc tggcggcctt cccgacgatg
     4621 ccggagaccc tgcaagtgat gctgagtaca gaagagttta accgcctgcg cgacgcggtg
     4681 ccggaaaaag tcagcgaatg ggggctgacg ccttcgccgg atctgccgcc aggcgaatgc
     4741 cgggtgatta ccgacaaatc tgaactggac atcggctgtg agcatcgcct tgaacagtgc
     4801 atgaccgcgc tgcaagagac gctaacgccg gagtcgcagg gtgagtgatt tttctgcttt
     4861 cgataacgcc ctgcgctcgc tggagtcgat cccgctggcc cgcgtcgccg ggcggctggt
     4921 gcgcctgaac ggcattctgc tggagagcgt cggctgcccg ctaatgaccg ggcaactgtg
     4981 ccgcatcgaa agcgccaatc acacactgat cgacgcgcag gcagtgggct ttaatcgcga
     5041 catcacctac ttaatgccgt ttaaacagcc ggtcggcctg atggcaggcg cacgcgtctt
     5101 cccggaagaa aaagcccacg acatcctgat tggcgaaagc tggttaggcc gcgtggttaa
     5161 cggtctcggc gaaccgctgg atggcaaagg ccgcctgaac ggcaacgacc ttctgccgcc
     5221 gctgccaccc tcggtcaatc ccttaacccg ccgttcagtg gacgaaccgc tggacgtggg
     5281 cgtgaaagcg attaacggcc tgctgaccat cggcaaaggc cagcgtgtcg ggctgatggc
     5341 gggcagcggc gtcgggaaaa gcgtgctgct ggggatgatc acccgccaga ctaaagccga
     5401 catcgtggtg gtcgggttga ttggtgaacg cgggcgcgaa gtgaaagagt ttatcgacca
     5461 ctcgctgggc gcagacgggc tagcaaagtc tatcgtcgtt gtcgcgcctg ccgatgaatc
     5521 gccgctgatg cgcctgaaag ccaccgaact gtgccactcc atcgccgcct ggtttcgtga
     5581 ccgcgggcat cacgttttac tgctggtgga ttccctgacc cgctacgcca tggcgcagcg
     5641 tgaaattgct ctctcactcg gtgaaccacc cgccaccaaa ggctatccac catcggcatt
     5701 cggcatgatc cccaaactgg tcgagagcgc aggcaacagc gaaagctcag gatcgatgac
     5761 cgccatctac acggtgctgg cggaaggcga cgaccagcaa gatccaatag tcgactgcgc
     5821 ccgcgcagtg ctggacggac atatcgtatt aacccgcaaa ctggcggaag cggggcacta
     5881 tccggctatt gatatcggcc agtcgatcag ccgctgcatg aaccaggtca cccgcctgga
     5941 acatcagcaa tccgcccgcg cgttaaagca aaactacgcc gcctatatgg agatcaaacc
     6001 gctgatcccg ctcggcggct acgtggcagg cgcggacgcc agcgtcgaca aagcggtgaa
     6061 aatgttcccg gctatcgagc gatttttgcg ccaggagatg cgcgaacccg cctccctcga
     6121 actggtgcaa agccgcctgc aaatcctctt tcccatcgcc aaaaaggcag aagggcaata
     6181 accatgcgac aaatcatcga caccctggcg cagttgcagc gcctgcggga taaatcggtc
     6241 aaagacaaaa ccgtcgagct ggcaaagcaa aaacagattt gcgctggcta tgataacaat
     6301 atcaaagcat taggttacct tgtcgagaaa acatctgcgg gcgcggcggc gtcggtggag
     6361 tcgctgaaaa acgtcagcgg ctacaaaggc acactgcgca aggtgatcgc ctggcaagag
     6421 caggaaaaaa cgctggccaa catcaaagct acgcgaatgc agaaaaacct gacggcggcg
     6481 gcgtgcgaag agaaagtggt agccctgacg ctggacgaca aacgccgcga gcaacaggaa
     6541 agcgccacgg cgaaagcgca aaaagcggtg gatgacatcg ccgtccagtg ctggttgcgg
     6601 cacaaattag cggagtagcc atgaaaacgg tcatcacctt cggcaccttc gatgtgtttc
     6661 atgtgggtca tctgcgcctt ttacaacgcg ctcgcacgct gggcgagcag ctgctggtcg
     6721 gcgtatcatc cgatgcctta aatatcgcca aaaagggcag ggctccggtt taccctcagg
     6781 atgaccgcat ggcgattatc gccgggctgg cgtgcgtgga tggcgtattt ctggaggagt
     6841 cgctggagca gaaagcggaa tatttgcgcg ggtacagtgc ggatattttg gtgatgggcg
     6901 atgactgggc aggtaagttt gacgatttct cgtgtgtatg tgaggtggtc tactttcccc
     6961 gcacgccgtc ggtatcgacg acggggatca ttgaggtgat tagggggcat ttctgataat
     7021 atgagttcct ctattttagg tattttgatg aatatttaaa aaatcataat tcaaatgaat
     7081 atagttaatg tttatgagtt tactgttctc tatttcatat tctttaatag agtcaatata
     7141 agatattaaa tcggaatttg atatctcaat aagagttgcc ggacgtataa ttccctttag
     7201 atggaaatta cagcaccagg catagtcacc agcatccttg attgaacgcc aaaatatatc
     7261 atattcatga caatagaaat aaattcttcc gtcattgaac tcagggatga aacaaaattc
     7321 tacaccgtca ctttgacgtt caattgcatt cttatcacga tgaacaaaat aaactttttt
     7381 catatgaata tttc
//
LOCUS       DAJOTB010000204         7358 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJOTB010000204 DAJOTB010000000
VERSION     DAJOTB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.204"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            276..1808
                     /locus_tag="OHN28_004432"
     CDS             276..1808
                     /locus_tag="OHN28_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCQ2299705.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(1860..2579)
                     /gene="fadR"
                     /locus_tag="OHN28_004433"
     CDS             complement(1860..2579)
                     /gene="fadR"
                     /locus_tag="OHN28_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCQ2299706.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2801..4342
                     /gene="nhaB"
                     /locus_tag="OHN28_004434"
     CDS             2801..4342
                     /gene="nhaB"
                     /locus_tag="OHN28_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCQ2299707.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4488..5018
                     /gene="dsbB"
                     /locus_tag="OHN28_004435"
     CDS             4488..5018
                     /gene="dsbB"
                     /locus_tag="OHN28_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCQ2299708.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5063..6331)
                     /gene="umuC"
                     /locus_tag="OHN28_004436"
     CDS             complement(5063..6331)
                     /gene="umuC"
                     /locus_tag="OHN28_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCQ2299709.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(6331..6750)
                     /locus_tag="OHN28_004437"
     CDS             complement(6331..6750)
                     /locus_tag="OHN28_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCQ2299710.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            6947..7336
                     /locus_tag="OHN28_004438"
     CDS             6947..7336
                     /locus_tag="OHN28_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HCQ2299711.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETARFYVDYDDLMLSLLKE
                     AAQKMINTCNEYQKRHGKKTLFEIPEV"
BASE COUNT         1809 a         1824 c         1793 g         1932 t
ORIGIN      
        1 gataactcag ttgaaaatat tattcaacat acggcttttt tatggtgagc tggctcacat
       61 ctccctgact ctgcggcaat atggcgtctt tagtatctcc tgctgaacgc cctttttatc
      121 cagcataaaa ttccgttcag aagcggatta gtggcactct gataataaga aatcgcaaag
      181 aaaaaaattc tccggcgacg cggtttttaa caggtgttct atgcttgaaa tgaggtgctc
      241 cacacagaga gcgccgacaa caatgaggtg cgcgtatggc gacgatcgat tctatgaata
      301 aggacaccac acgtttgagc gatggacccg actggacgtt cgacctgctg gatgtttatc
      361 tggcagagat agaccgggtg gcgaaactct accggctgga tacctacccg caccagattg
      421 aagtgataac ctcagaacag atgatggatg cttactccag cgtcggcatg ccaattaact
      481 atccgcactg gtcattcggt aaaaagttta tcgagactga acggctgtat aagcacggtc
      541 agcaaggact ggcctatgaa atcgtcatta actctaaccc gtgtatcgct tacctgatgg
      601 aagagaacac cattaccatg caagcgttgg tgatggctca tgcctgctat gggcataact
      661 ctttcttcaa aaacaattac ttattccgta gctggaccga cgccagttcg attgtcgatt
      721 atctgatttt tgcccgtaaa tatattaccg agtgcgaaga acgttatggc gttgatgaag
      781 tagaacggct tctggactcg tgccacgcgc tgatgaacta cggcgtggac cggtacaaac
      841 gcccgcaaaa aatctcgctg caagaagaga aagcccggca gaagagtcgc gaagagtatc
      901 tgcaaagtca ggtcaatatg ctctggcgta ccctgccgaa gcgcgaggaa gagaaaacgg
      961 ttgctgaagc gcgccgctat ccgtccgaac cacaagaaaa cctgctctat tttatggaga
     1021 aaaatgcgcc actgctggaa tcatggcagc gtgaaatcct gcgtattgtg cgtaaggtga
     1081 gccagtattt ttatccgcaa aaacagactc aggtgatgaa cgaaggctgg gcgaccttct
     1141 ggcactacac catccttaac catctgtatg atgaagggaa agtaacggaa cgttttatgc
     1201 tggagttttt gcacagccac accaatgtgg tcttccagcc gccctataac agcccgtggt
     1261 acagcggcat caacccatat gccctcgggt tcgccatgtt ccaggatatt aaacggattt
     1321 gtcagtcgcc gacggaagaa gacaaatact ggttcccgga tatcgccggt tccgactggc
     1381 tggaaacgct gcatttcgcg atgcgtgatt tcaaagatga gagttttatc agccagttcc
     1441 tgtcaccgaa agtgatgcgt gatttccgct tcttcaccgt gctggatgat gatcgacata
     1501 attatctgga gatttccgct attcataatg aagaaggtta tcgggaaatc cgtaaccggt
     1561 tatcgtcgca atataactta agtaatctgg agccgaatat tcagatctgg aacgtggatt
     1621 tgcgcggtga ccgttcgctg acgctgcgtt acattccaca taatcgcgca ccgctggatc
     1681 gggggcgcaa agaagtgctg aagcatgtgc atcgcctgtg gggatttgat gtgatgctgg
     1741 aacagcaaaa cgaagacggc agcatcgagt tgctggaacg ttgcccgccg agaatgggaa
     1801 atctgtaaaa acaacgaaaa acccctcgtt tgaggggttt gctttttaaa cggaagggat
     1861 tatcgtccct gaatggctaa atcacccggc agatttttct gcatccggtg ccaaatctcg
     1921 ccactctcat gcccatagcg acgcactgtt tcatatacct gatcgtgtgc gccttcactg
     1981 cacaacgccg acagtttgtg atagaagccc agcgccagac tgcgcgcttc cggattggcg
     2041 aaatagtgac gaccaatgcg cgtatacagc cctttcatcc cgttaaggat cagaccgtaa
     2101 atcgggttgc cggaagcaaa cgccaggccg cggaaaatgt tgtaatccag ctcggcaaag
     2161 gcgtcggcgt gatcggccac ttcattagcg gttgccagca cttcctgcgc tttatcggga
     2221 tgttgacgaa acgcggtgcg aataaaaata gttgaaatat tggtacgcac cgacagcaaa
     2281 ttatcaataa gctgcggcac actttcgtgg tccagtcgcg ccagtgtttc aaggatattt
     2341 aaaccagagg tttcccagaa attattcacc ttcgtcggct tgccgtgttg aatggtcaac
     2401 cagccatctc gtgccagacg ttgtaacact tcacgtaacg tagtacgcgt tacgccaatt
     2461 aattctgaaa gttcacgttc tgcgggcaaa atagtcccgg gagggaagcg gttattccag
     2521 atactttcaa taatgtactc ttccgcgaaa cccgccgggc tttgcgcctt aatgaccata
     2581 gtgagatttc cataacacag caaaacaaag ttggactcat cataccagag ggctaactac
     2641 gctgatagaa gacaaaaaga agaaaaaagg gatcaaggct tcattttctg catctctttc
     2701 cccttgacta tcaagacttg cctcactcct gtctgaccgt taagctttgc gtttaaacat
     2761 aaaaacatca ttgattattt ctaaggtaag ggaaaccatc atggagatct cctggggccg
     2821 cgcactatgg cgcaattttt tgggccagtc ccccgactgg tacaaactcg ccctcatcat
     2881 tttcttaatc gtaaatccgt taattttcct catcagccct ttcgtcgctg gctggttgct
     2941 ggtagcggaa tttattttca ctctggcgat ggccctgaaa tgctacccgc tgctccccgg
     3001 tggtctgttg gctatcgaag cggtattcat cggcatgacc agcgcggaac atgttcgtga
     3061 agaagtggcg gcaaatcttg aagtcttgct gttactgatg tttatggtgg cgggtatcta
     3121 ttttatgaaa cagctgttgt tgttcatatt tacccgtttg ctgttaagca ttcgctccaa
     3181 aatgctgctg tcgctctctt tttgcgtggc ggcggcgctc ctctccgcgt tcctcgatgc
     3241 cttaaccgtc gtggcggtgg tgatcagcgt tgcagtcggt ttttatggta tttatcaccg
     3301 cgtagcctct tcccgtactg aagataccga cctgcaagac gatagtcata tcgacaagca
     3361 ttacaaagtg gttctggaac agttccgtgg ctttctgcgt agtctgatga tgcatgccgg
     3421 tgtcggcacc gcattaggcg gcgtgatgac catggtgggc gaaccacaga acctgatcat
     3481 cgctaaagcg gctggctggc attttggcga tttcttcctg cgcatgtcgc cggtgaccgt
     3541 cccggttctg atttgtggcc tgttaacctg cctgctggta gagaagctgc gttggtttgg
     3601 ctacggtgaa acgctgccgg agaaagtccg cgaagtactg caacagtttg acgatcaaag
     3661 ccgcaaccag cgtacccgtc aggataaaat ccgtctgatt gtccaggcga ttatcggcgt
     3721 ctggctggtg actgcgctgg cactgcatct ggcggaagtt ggtttgattg gtttgtcagt
     3781 cattattctg gcgacatcat tgaccggtgt taccgatgaa catgctatcg gtaaagcctt
     3841 caccgaatct ctgccattca ccgcactgtt gacggtgttt ttctcagtag tcgcggtgat
     3901 tatcgaccaa caactgtttt cgccaattat tcagtttgtg ttgcaggcat ctgaacatgc
     3961 tcagctgtcg ttgttctata ttttcaacgg tctgctgtca tccatttcgg ataacgtctt
     4021 cgtggggacg atttatatca acgaagcgaa agcggcaatg gaaagtggcg ctatcacgtt
     4081 gaagcaatat gagctgctgg cggtcgccat taataccggt accaatctgc cctccgtcgc
     4141 tacgccgaac ggtcaggctg cgttcctgtt cctgctgacc tctgcactcg cgccattgat
     4201 tcgtctctct tatggccgca tggtgtggat ggccctgcct tacaccctcg tactgacggt
     4261 tgccgggtta ctctgcgtcg agtttacgct tgctcctgta accgaatggt ttatgcaaat
     4321 gggctggata gcaacgcttt gataacaact taccgggcat ataaatgccc ggtttgcctt
     4381 ttcgcccgat aattgtccaa ttgcgtccta tttttgctgc ctcctggtgg cgcagcgaat
     4441 gaattggttt aaactgcgca ctctatgcat attgcaggga aatgattatg ttgcgatttt
     4501 tgaaccaatg ttcacaaggc cggggcgcat ggctgttgat ggcgtttact gctctggcac
     4561 tggaactgac ggcgctgtgg ttccagcatg tgatgttact gaaaccttgc gtgctctgta
     4621 tttatgaacg ctgcgcgtta ttcggcgttc tgggtgctgc gctgattggc gcgatcgccc
     4681 cgaaaactcc gctgcgttat gtagcgatgg ttatctggtt gtatagtgcg ttccgcggtg
     4741 tgcagttaac ttacgagcac accatgcttc agctctatcc ttcgccgttt gcgacctgtg
     4801 attttatggc tcgtttcccg gaatggctgc cgctggataa atgggtgccg caagtgtttg
     4861 tcgcctctgg cgattgcgcc gagcgtcagt gggaattttt aggtctggaa atgccgcagt
     4921 ggctgctcgg tatttttatc gcttacctga ttgtcgcggt gctggtggtg atttcccagc
     4981 cgtttaaagc gaaaaaacgt gacctgttcg gtcgctaatc cattcggcgc tcctgcggga
     5041 gcgctttttc ctgccgctat gtttatttga cccgcagtaa atcagaactt cgcgttgtat
     5101 aacatggtga aagcatggca cgcttcatct gccattgttg ctggatcccc tgcccggcaa
     5161 aatagagtgt tcctctgccc tctttagcat tcagcgtatc cattaccgcc atcaattgct
     5221 cactcccggg gcgtggtgcg ttgtcatcga ataaattgag ctgcgcgact ccctgactga
     5281 agaaatcccc cagcatcacg cccgcttttt ggtagcgatg gcccgcttgc cagatggcat
     5341 ccagagatcg cgtagcggcg ttaatgatat ccctgctgtc ctgagtgggc gtcagcagtt
     5401 ttaccgatgc gctattgccg taataaggtt cattgaacgc aaatggtgac gtcttaataa
     5461 acgtggagat aaaccgacaa tattgatgct cgctgcgaag tttttccgcc gcccgggcag
     5521 cgtaactaca aatggcctgc cgcatcgacg gataatccgt gatgcgttca ccaaacgagc
     5581 gggaacagat aatttcctgc ttcgtcggtg caaactcttc cagttgcaaa cagggttcac
     5641 cgcgcagttc acgcaccgtt ctttcgagca cgacattaaa atgtttacgg ataaaccgga
     5701 tatctgaatc tgccaaatct agaacggttt tgatccccat cgcgtccagt tttttgctga
     5761 tccgccgtcc aatcccccag acttcatcca cggggagagc agacattaat ttacgctggc
     5821 gttccagatt tgataaatcc accaccccac ccgtctgccg ctgccatttt tttgccgcat
     5881 gattggcaag cttagccagc gttttggtct gggcgatccc cacaccaaca gtaagatggg
     5941 tacgttgtag caccgttgcg cgaatttctc tgccaaaatc agtcagatcg cgacaattat
     6001 gcacacctgt cagatcgcag aatgcctcat caatactgta aatctcgacg cggggcgata
     6061 gttcttccag cgtcgacatc acccgattac tcatgtctgc gtaaagctca taattgctgc
     6121 taaagcaaac cacgccacag cgacgaaaca gatctttttg tttgaaccag ggatcgccca
     6181 ttttaacgcc cagcgccttt gcctcagcgt ttcgggcgat aacgcaaccg tcattattcg
     6241 atagcacaac caccggttta ccccataaat cagggcgaaa caccgtctca cagctggcat
     6301 aaaacgcgtt tacatcacag agggcaaaca tcagcgcatc gccttaacga cgtggatcac
     6361 cacaccaaag acatccagcg tatcttcgct actgatggta atgggcgagt acgcactgtt
     6421 cataggaata agctgtaccg tcgggcgtag ttgcaatttt ttcaccgtaa actcgccgtc
     6481 aacagcagcg atgataatat caccatggct ggcggtaata gcgctatcga caatcagtaa
     6541 atcaccgtca ctaatcccac catcaatcat agaatcacca cttgctttga cgaaataagt
     6601 cgcgctggga tgctggatca acagttgatt cagatcgatg cgctgttcaa cgtaatctgc
     6661 ggccggtgaa ggaaagccac actgaacaag atcgctaaat agcggaaaag tcacaatttc
     6721 gcggagatcc gcaggcttga taaacaacat aataatctgc ctgaagttat actgttttta
     6781 tatacagtag tctgttcttg tcagcagatc aatactgatt caggctatca atatttgtcg
     6841 ctgcatagac tgctgatttt tcgttctctt atcctgtgct cacgcggcct tctggcgaca
     6901 gcgtccatcc agcagaaagg aaaaatatca cctggtttaa aatagaatcg aagcattcgc
     6961 ccagaaaggc attctcatta aggtactgga tgacggcatc aagaaactgt gttctattgc
     7021 tgcgggcgta gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca
     7081 gagtttcttt accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc
     7141 caaattgaaa ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac
     7201 agccagattc tacgttgatt atgatgattt aatgctttct ttactaaaag aagcggccca
     7261 aaaaatgatt aacacctgta atgagtatca gaaaagacac ggtaagaaga cactctttga
     7321 gatacctgaa gtctgataag cgattattct ctctatgt
//
LOCUS       DAJOTB010000205         7321 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJOTB010000205 DAJOTB010000000
VERSION     DAJOTB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.205"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            514..1428
                     /gene="chuT"
                     /locus_tag="OHN28_004439"
     CDS             514..1428
                     /gene="chuT"
                     /locus_tag="OHN28_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HCQ2299712.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1448..2785
                     /locus_tag="OHN28_004440"
     CDS             1448..2785
                     /locus_tag="OHN28_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HCQ2299713.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLVQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDVCAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2798..3292
                     /gene="hutX"
                     /locus_tag="OHN28_004441"
     CDS             2798..3292
                     /gene="hutX"
                     /locus_tag="OHN28_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HCQ2299714.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            3292..3915
                     /gene="chuY"
                     /locus_tag="OHN28_004442"
     CDS             3292..3915
                     /gene="chuY"
                     /locus_tag="OHN28_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HCQ2299715.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            4000..4956
                     /gene="chuU"
                     /locus_tag="OHN28_004443"
     CDS             4000..4956
                     /gene="chuU"
                     /locus_tag="OHN28_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HCQ2299716.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4953..5723
                     /locus_tag="OHN28_004444"
     CDS             4953..5723
                     /locus_tag="OHN28_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HCQ2299717.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5775..6422)
                     /locus_tag="OHN28_004445"
     CDS             complement(5775..6422)
                     /locus_tag="OHN28_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCQ2299718.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6486..6812)
                     /gene="hdeB"
                     /locus_tag="OHN28_004446"
     CDS             complement(6486..6812)
                     /gene="hdeB"
                     /locus_tag="OHN28_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCQ2299719.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6928..7260)
                     /gene="hdeA"
                     /locus_tag="OHN28_004447"
     CDS             complement(6928..7260)
                     /gene="hdeA"
                     /locus_tag="OHN28_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCQ2299720.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1846 a         1830 c         1761 g         1884 t
ORIGIN      
        1 acgccctctt tgtcaggcaa agtagtacgc cacgtctgaa gtgaagagtg ctgtgcgttt
       61 tcccttacaa aagatatgat tttgttatcc atgatgtcat attttgttaa actcaaacgt
      121 gataatgaga attattatcg atcaattttt tatgatcaag ctttttttaa ccgccattaa
      181 cacaataaac agggggttat gaaatctatc cagcatccag aaaaccatcc tgtgtttatg
      241 gctatccccc cacaacctca tcacaaaacg ttgtcgttaa cagtgataat tttctcttct
      301 ctttgctctg caagccgcga tatctccggg ttctcagctt tttcccgcgc tgaaatttaa
      361 tcggtaacaa gaacattagc tgacgaagca tcttgataaa aatcaaaaat aaatacgctt
      421 gtaacgccgt gcgtattgaa tcgacggttg tatttcatat ataactcaaa tgataatcat
      481 gatcattctc aatttgataa gagttctcac attatgccaa ggatcatcac caggccgttt
      541 cttttttccc cactaacgtt atgtatttca gccgtggtct ctgccgcgaa gaccatggta
      601 aaacgaaaaa aattgtttac ggcattactg gctcttagct ggacttttag cgtaacggct
      661 gccgaacgta tcgtggtcgc aggaggatcg ctgacggagc taatctacgc gatgggcgct
      721 ggcaaacgcg tggttggtgt cgatgaaacg acatcctatc caccagaaac cgccaaactg
      781 ccgcatattg gttactggaa acaactcagc agcgaaggca ttttgtcact tcgcccggat
      841 agcgtaatta cctggcagga tgcaggaccg caaattgtgc tcgaccaact gcgggcgcaa
      901 aaagtcaacg tcgtcactct gccgcgtgta ccagccaccc ttgagcagat gtacgccaac
      961 attcgccaac tggcaaaaac gttacaggtt cctgaacaag gcgaggcgct ggtgacacag
     1021 atcaaccaac gcctggagcg agtacagcaa aacgtggcgg ccaaaaaagc cccggttaaa
     1081 gccatgttta ttctctccgc aggcggaagt gcgccacagg ttgccggtaa aggcagcgtc
     1141 gcggacgcca ttctttcgct tgccggagca gaaaacgtcg ctacccacca gcagtacaaa
     1201 agctacagcg cggaatcgct gattgcggct aaccctgaag tgattgtcgt cacctcacaa
     1261 atggtcgatg gcgatattaa tcggctacgt tctattgccg gaattaccca cactgccgca
     1321 tggaaaaacc agcgcattat caccgttgat caaaacctga ttctgggaat gggtccgcgt
     1381 attgctgatg tcgttgagtc tttacaccag cagctttggc cgcaataaat ggatttaaat
     1441 cagacgaatg aacacaaata acaccctgga tctcacgcca cattttgctt tagacggtga
     1501 tcagcccttt aaagacaggc gcgccatgat gccgtttcgt ggggccattc cggttgccaa
     1561 agagcaactg gtacagacct ggcaagagat gatcaatcaa acggtatcac cacgtaagcg
     1621 actggtttac ctacacattc ctttttgtgc gacacactgc acgttttgtg gtttttatca
     1681 gaaccgtttt aatgaagatg tgtgtgctca ttataccgac gccctgattc gtgaaataga
     1741 actggaagca gacagcgtat tgcatcagtc gggccccatc cacgcggtct atttcggtgg
     1801 cggtacgcct tccgcgcttt cagcacacga tttggccaga atcatcacca cattacgaga
     1861 aaagttgcca ctggcccctg attgtgaaat cactatcgaa ggccgggtac tgaattttga
     1921 cgctgagcga atcgatgctt gccttgatgc tggagcaaac cgcttctcaa ttggtattca
     1981 gtcgtttaac agcaaaatcc gcaagaaaat ggcccgcacc tcggatggcc caaccgctat
     2041 tgcgtttatg gaaagcctgg tcaaacgcga ccgtgccgcg gtggtctgtg acctgctgtt
     2101 tggtctgccg ggtcaggatg cgcaaacctg gggagaagat ctggctattg cccgcgatat
     2161 cggtctcgac ggcgtcgatc tctatgcgct caatgtcctg cccaatacac cgctgggcaa
     2221 agccgtggaa aatgggcgta ctaccgtgcc ctctccggca gaacgtcgcg atctttacct
     2281 gcaagggtgt gattttatgg acgatgccgg ttggcgctgc atcagtaaca gccactgggg
     2341 ccgtaccaca cgcgaacgca atctctataa cctgctgatt aaacaaggtg ccgattgtct
     2401 ggcctttggt tccggagccg gcggttcgat taatggttac tcctggatga acgaacgcaa
     2461 tcttcagacc tggcatgaat ccgtcgcagc aggcaaaaaa ccgctgatga tgatcatgcg
     2521 taacgccgaa cgtaacgcgc agtggcgtca taccttgcag tcgggcattg aaacggcgcg
     2581 tgtaccgctg gacgaactta cgccacatgc cgaaaaactc gcgccgttac tggcccagtg
     2641 gcaccaaaaa ggcttaagcc gcgatgcgtc aacgtgtctg cggttgacca atgaaggtcg
     2701 tttctgggca agcaacattt tgcagtctct taatgaactg attcaggtac ttaatgcgcc
     2761 agcaattgcg cttgaaaaac cataacagga gtgatttatg agccatgtct cgttacagga
     2821 atttttgaaa acggagccag acggtacgct ggaggcgatc gccgaacaat acaacaccac
     2881 gctgctggag gtagtgaaaa atctgccctc tccgactgtt gtgtcaggtg ataaattcga
     2941 tactgtctgg gataccgtct gcgaatgggg caacgtcacc acgttagtac atactaccga
     3001 tgtaatactc gaatttagcg gcgaactgcc ttcgggtttc catcgccacg gctatttcaa
     3061 cctacgcgga aagcacggca tgtccgggca tatcaaagca gaaaactgca cgcatattgc
     3121 cttgatcgaa cgtaaattta tgggcatgga caccgcctct attctctttt tcaataagga
     3181 aggcagcgca atgctgaaaa tcttcctcgg tagggatgat catcgtcagc tcctgagcga
     3241 acaagtcagc gccttccatg ctctggcagc atcgttaaag gaacacgcct gatgacaccg
     3301 tggctactct ttggtgcggg cggaaaaggt gtgggtgcca gaacgcttga actggcgctg
     3361 gcggaacaac gtccggtcgt tgctgtcatt cgtcatgccg atgccgccac gaaactggcg
     3421 caacaaggcg tacaggtttt tacaggcgac gcctgtgatg ccagcgtagt cgcggctgct
     3481 tgccgcgcag ctggcccgga tgcgcttatc atctcaacaa tgggcggagc tcaggattat
     3541 ctggcgcaca gaacagtgat tgatgaggcc gagaaggctg gcatcagtcg catgattctg
     3601 gtgacctctt tgggatgcgg cgatagctgg ccgtttttat cagagcgggc aaaggctgcg
     3661 tttggtcagg cggtacggga aaaaacgctg gcggaaagct ggttgcaaac cagccagctt
     3721 gattatgcca ttctccgccc tggcggtctg cttgatggtg cggcaacggg caaagcacaa
     3781 cggatacaaa atcaggagtg tcatggtttt gttaaccgtg ctgatgtcgc cgcacatatc
     3841 catgaactgg caaatgcgcc agcacttaac cagcaggtct acagtctgat tgaaccggac
     3901 ctgaaaccgg cgtgatcccc cggcgcggca tgtccgcgcc gtaatgtcgt aacggagttt
     3961 tgcgtgctca aggattcact ctcttccgcc agggtcttta tggggctatc actattattg
     4021 ctagctctgg tgctgtttgg tgccagtcag ggagcgttaa agatcagttt tgatgccctt
     4081 tttgatgagg aataccgcga tatctggctc aatattcgtc taccacgggt tttgctggcg
     4141 gtgctggtag gtgcagcgtt ggcaaccgca ggggtaatta tgcaagggct ttttcgcaac
     4201 ccaatggctg accctggatt acttggcgtc agtagcggtt ctgcattaat ggttggcgtt
     4261 gctattgtgc tgcccttctc cttcccagtg gtgctggtgc tctatgagca aatggtgttt
     4321 gccattgccg gaagtttagt ggtctgcacc atcatttttc tcatcacgca gcgccatcgc
     4381 gatggcagca tgatgcaatt attactcgcc ggtatcgcca tcaatgccct gtgcggcgca
     4441 gcgatcggca tcctgagcta tatcggcgat gagcagcagc tacgacaact cacattgtgg
     4501 atgatgggca atcttggaca ggcgcaatgg ccgacgttat tggttgccag ttcattcatc
     4561 ctaccggcca ttatcgcaac aacttgtctc gccggaacgc tgaatttact gcagctcggt
     4621 gatgaagaag cccactacct cggcgtgaac gttaagcgta aacgccagca attactgtta
     4681 gtgagctcac tgctcgttgg tgctgccgta tcggtaagcg gtattatcgg ctttattggc
     4741 ctggtgatcc cgcatctgat tcgcatgact accggggcaa atcaccgctg gctaatccct
     4801 tgttccgccc tcgccggagc ctgtttattg ctgatggcag acacgcttgc ccgcacgctg
     4861 gtacagccag cagaaatgcc cgtgggatta ttaaccagcc tgcttggtgg cccttatttt
     4921 atgtggttga ttctgcgcaa ccggaggatc acatgatcag cgcccagaat ctggtttata
     4981 gccttcaggg gcggcgtctg actgataatg tctcgctgac ttttcccggt ggggaaattg
     5041 ttgccatcct cggccctaat ggtgcgggaa aatctacgct gcttcgccag ctaaccggct
     5101 atcttcaacc tgatagcggc gaatgtcggt tgtttaataa gccattaaat gaatggtcga
     5161 ttactgaact ggcgaaacac cgggccgtga tgcgtcaaaa tagccacatg gcgtttccat
     5221 tcagcgtaca agaagttatc cagatggggc ggcatccgca tcgtactggt aatcaggata
     5281 atgaaacagc acaaataatg gcgttatgtg actgtcaggc gttagcaaac cgcgactatc
     5341 ggcaattatc cggtggtgaa caacagcgcg tgcaattagc gcgcctgctg gtgcaactct
     5401 gggaacctac tccctcaccg aaatggttat ttcttgatga accaacttcg gcgctggata
     5461 ttcaccatca gcagcacttg tttcgtttat tacgccagtt agttcacgaa cggcagttca
     5521 atgtttgctg cgtattgcac gatctaaatc ttgccgcgcg ttatgcagac cgtgttgtat
     5581 taatgcaaaa aggaaaggtt atcgcaaatg gtaagccgca ggacgtattg acacagcagg
     5641 cgctgacaat gctctacggc gcggatatta ccgtacttaa agatcctgcc aaccattcac
     5701 cactgatcgt cctcgatcat taaaaccaac aggagtaccg cactggtact cctgcgaata
     5761 aataactata cagattagct aattgaaacg cctttcaccc ctgccatacc ttttaataat
     5821 cgcaacagat cttctatcga cgtcgtggca tgaagctgga tatcaatggc gaccacttca
     5881 tgatcttcat tttcctgcaa cgaaactaaa tccgttttta ttttttgctg cttaaaccag
     5941 tcgagcattg ataccgtatt accgttcact aatgtcagct gtaaatggta atttttgttc
     6001 atcaggcgga aggttaattg atggaagact tccagcacca gcaaggtcat cactgaacca
     6061 taaataccca gttcgtacat accgctgcca ataaccatac caatggcggc ggtcacccag
     6121 atatccgctg ccgtcgtcag acctacaata ttttggttac gaacgagaat gttacctgca
     6181 ccgataaacc cgacgcccgt cacaacctga gcagcaatac ggctggggtc tagtccgacg
     6241 tgacctaaag acagcacgtc agcaaaacca tatttcgaaa caatcataaa cagggcgctt
     6301 cccatgccaa ttaatacatg tgtgcgtaac cctgctcctt tgccgcgcat ttgtctttcc
     6361 atgccaatag cgccacaggc aattaccgca agtataagtc gtataataaa ttctgctgtc
     6421 atataaaaat gcaaaaagga gcagcaagat ggctcaactt gctactcctt tttacttgta
     6481 cctcattaat tcggcaagtc attagatgct tgatttttga aggtatacaa atttttctgc
     6541 gggtttttct tgcagtattc gatcacttta ggaatttgag tgagatcggt ttcatttaaa
     6601 gtaacggtat cgccaccttt atatactgtt tcttcatgca gcatccacca cgcaactggt
     6661 gtcattgctt ttggattcag atcaataaat tcctggcagg tcatatcttt agcggattca
     6721 ttggctgcca gcgcagattg tgcgttcacc agtgacaaag ccgctacagc gcccataaaa
     6781 ataaacgctt tacggagaga tgaaatgttc attttgtaac ccattcaata tagagattat
     6841 atacaaacag gaggaatagc tgcttttaaa gaagatattc cggcctgttc aatatgcaag
     6901 gaaatacgat gtaacgcatt cccggaatta catatctttc ttaattttgt cccattcgcc
     6961 tttaacttta tctttaaagc tggctttttt atcctgagta caagcctgaa cgatagctgg
     7021 ggttacggtt gcaataccct gaacatctaa aaccgcatct tctggtttat ctttgttgtt
     7081 cagtgcttca gcaaaaccaa ctgcagttgg ctggaaggat tcgtccacag ccaggaaatc
     7141 ttcacaggtc caggagttga ccggtttttt gttatcagct gctttttgtg catccgctgc
     7201 attgctcact actggcagaa gaagcagacc accaagaata acgcctaata cttttttcat
     7261 cgtaatatcc tcaactataa agtgaaagag ccgtcacgaa tcaatttcga cactgaggtt
     7321 a
//
LOCUS       DAJOTB010000206         7262 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJOTB010000206 DAJOTB010000000
VERSION     DAJOTB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.206"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1367
                     /locus_tag="OHN28_004448"
     CDS             <1..1367
                     /locus_tag="OHN28_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2299721.1"
                     /translation="EKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYLM
                     VMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRL
                     TSSVVQNDIAIYYLFRQMSLCMLIFLALINKVSENTKQRNLFSKKVTLCISLFFVLGG
                     PIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRCD
                     IWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRV
                     TKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGD
                     QVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGDN
                     PEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILINR
                     KDDE"
     gene            complement(1472..1559)
                     /locus_tag="OHN28_004449"
     tRNA            complement(1472..1559)
                     /locus_tag="OHN28_004449"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1523..1525),aa:Ser,seq:gga)
     gene            1793..2731
                     /gene="ghrA"
                     /locus_tag="OHN28_004450"
     CDS             1793..2731
                     /gene="ghrA"
                     /locus_tag="OHN28_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCQ2299722.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2786..3523
                     /gene="ycdX"
                     /locus_tag="OHN28_004451"
     CDS             2786..3523
                     /gene="ycdX"
                     /locus_tag="OHN28_004451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCQ2299723.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3547..4101
                     /gene="ycdY"
                     /locus_tag="OHN28_004452"
     CDS             3547..4101
                     /gene="ycdY"
                     /locus_tag="OHN28_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCQ2299724.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4203..4694
                     /locus_tag="OHN28_004453"
     CDS             4203..4694
                     /locus_tag="OHN28_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2299725.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(4759..5592)
                     /gene="csgG"
                     /locus_tag="OHN28_004454"
     CDS             complement(4759..5592)
                     /gene="csgG"
                     /locus_tag="OHN28_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCQ2299726.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5619..6035)
                     /gene="csgF"
                     /locus_tag="OHN28_004455"
     CDS             complement(5619..6035)
                     /gene="csgF"
                     /locus_tag="OHN28_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCQ2299727.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6060..6449)
                     /gene="csgE"
                     /locus_tag="OHN28_004456"
     CDS             complement(6060..6449)
                     /gene="csgE"
                     /locus_tag="OHN28_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCQ2299728.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6454..7104)
                     /gene="csgD"
                     /locus_tag="OHN28_004457"
     CDS             complement(6454..7104)
                     /gene="csgD"
                     /locus_tag="OHN28_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCQ2299729.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1881 a         1580 c         1746 g         2055 t
ORIGIN      
        1 tggaaaaaga ctatttggga attagtagta ctgtattagt gagcttattg tttgggcttg
       61 cattggtttt ggttaatagt tggttcaatc agccaaccgt tgaggaagtt gttccacgat
      121 caacctatct gatggttatg attgctttat tttttatcga tactgttgca tttattttta
      181 tgcagttgta tttcatttat gaccgtaggc aattttcaaa ctgtatactt agtttggctt
      241 ttctaagttg tttgatttac tttgttaaaa ccgtcattat cattcagcaa attattgagg
      301 ggcgtttgac aagcagtgtt gttcaaaatg acattgcaat ttattatttg tttcgtcaga
      361 tgagtttgtg catgttaata tttctggcat tgataaataa agttagtgaa aacacaaaac
      421 agcgcaattt attttccaaa aaagtgactt tgtgcataag tttgtttttt gttcttgggg
      481 ggccgatagt tgctcacata ttttctagcc attatgagag ctacgattta cacattgccg
      541 agttaaccaa tgaaaatggt caggtggttt ggaaagcctc atatgttacc ataatgattt
      601 tcatgtggtt aaccttgctt tcggttaatc tatactttaa tggtttacgc tgtgatatct
      661 ggaacggggt gtcggtgatt gctttttgtg ccgtgctgta caatatctct ctgttattta
      721 tgagcaggta tagcgtgtcc atttggtata ttagtcgcac tattgaagta gtcagtaagt
      781 taaccgtttt ggtaattttt atgtgccata tattcagtgc gctacgagta acaaaggaca
      841 ttgcacatcg cgatccctta accaatatat tcaacagaaa ttattttttt aatgaactga
      901 cggttcaatc agcatcggcc aaaaaaacac cttattgcgt catgattatg gatattgacc
      961 acttcaaaaa agtcaacgac acctggggac atccggttgg cgatcaagtg ataaagacag
     1021 tggtgaatat cattgggaaa agcatacgac cagatgacct tttagcgcgc gttggcggcg
     1081 aagagtttgg cgtcttgctg acggagatcg atactgaatg tgcgaaagct ttagcggaaa
     1141 ggattcggga aaatgttgaa cgtttaactg gcgataatcc tgaatacgcc ataccacaaa
     1201 aagtgacgat tagtattggc gctgttgtaa cgcagaaaaa tgagttaaac cctaaggaga
     1261 tctatcaact ggctgataac gcactttacg aggcgaaaga gaccgggcgt aataaggtgg
     1321 tagtaaaaga ggcagaaatt cttatcaacc gcaaggatga tgaatgagtc tgtcgctagt
     1381 tgtcaacctc cgcctggggc ttctcatccc cccggtgtat gcaatatacg aaaaaaaagc
     1441 tcgtactttc gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg
     1501 atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca
     1561 aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg acctgaggtc
     1621 aacaaacttt tttaaaaaat cgctcgatta ttcaaacttc aatcaatatg tagttttaat
     1681 aagcgaaatt tgcttttttt gtcaccgacc acggatttgt tatgctggtg gcctttgtag
     1741 atcataacga taagtgcgaa taaatttcgc gcaacgcttt tcgggagtca gtatggatat
     1801 catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc acaaagctat
     1861 tcctcaggca agagtcagag catggaaaag cggagacaat gaatcagccg attatgcttt
     1921 agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgtttgcact
     1981 tggggcaggt gttgattcta ttttgagcaa gctacaggct caccctgaaa tgctgaagcc
     2041 ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc
     2101 tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat taccgcctcc agcaaaatag
     2161 ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg
     2221 cgcaggcgta ctgggcagta aagttgcaca gagtctgcaa acctggcgct tcccgctgcg
     2281 ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga
     2341 actttctgca tttctgagcc aatgccgggt attgattaat ttgttaccga atacccctga
     2401 aaccgtcggc attattaatc aacaattact cgaaaaatta ccggatggcg cgtatctcct
     2461 caacctggcg cgtggtgttc atgtcgtgga agatgacctg ctcgcggcgc tggatagcgg
     2521 caaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc cggaaagtcc
     2581 gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc
     2641 tgaagctgtg gattacattt cgcgcaccat tgcccagctc gaaaaagggg agagggtctg
     2701 cgggcaagtc gaccgcgcac gcggctacta ataaagcatc aggtttcctg ctatccttgg
     2761 cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc
     2821 cagcacgcat gcatatagca cattgagtga ttacattgcc caggccaaac aaaagggcat
     2881 taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc atcactggca
     2941 cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat
     3001 cgaagctaac attaaaaatg ttgatggtga aattgattgc agcggcaaaa tgtttgattc
     3061 gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg acaaagcaac
     3121 caatacacaa gcgatgatcg ccactatcgc cagcggcaat gtgcatataa taagccatcc
     3181 cggaaatccg aaatatgaga tagatgttaa agccgttgct gaagcagccg cgaaatatca
     3241 ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca gtgaagacaa
     3301 ctgtcgtgcg gtagctgcag cggtacgtga cgctggaggt tgggtggcat taggctcgga
     3361 ttctcacact gcatttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt
     3421 agattttccg ccagaacgca ttttgaatgt ttctccgcgc cgcttactga acttccttga
     3481 atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat
     3541 atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa
     3601 ccgcaagatc ctttactggt gccgttgttt accctgattc gtgaggggaa actggctgcg
     3661 aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg tgatatgacg
     3721 caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgctgt gccgccatat
     3781 cgtagcgcat gggttgaggg cgcaacggaa gcggaagtgc gcgcttttct ttccgaacga
     3841 gggatgccac tagcggatac gccagccgat cacatcggca cattgctgct tgcggcttcc
     3901 tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag
     3961 tatctgttac cctggtgtgg tgcgttcctt ggcaaagtgg aggcccatgc aactacgcct
     4021 ttctggcgca ccatggcacc gctaacccgc gatgctatta gtgcaatgtg ggacgagctg
     4081 gaagaagatt ctgaagagta aatgtgatct gcatcactca taactgcaac tgataatttg
     4141 ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg
     4201 gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt atctggggat
     4261 gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt acggcctact
     4321 ttgcctgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg
     4381 gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat ctggaaattc
     4441 tcggttatgt aataaccggt attgtcgcgt ttctgatgtg tattcaggcc aaacagctgc
     4501 tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcaggggg
     4561 actggaaact ggtgttacct tcgctggcgc ttggactggt atttggttac gcaatgaaaa
     4621 acagcggcct gtggctggcg gcgcgtagta cgaagaccgc tcaccgtgag cagcaaatca
     4681 aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaaatgcc ggattattaa
     4741 ttccggcgtt tttatctatc aggattccgg tggaaccgac atatggcggt atttcaccag
     4801 aatgtcattc tgccgttctg ttttattttg caaatcccaa aggccgcggt cgataccatc
     4861 attaatcagg aaaatgacgc ctgtttcgat agccgacatc aggcacagca taacgggttc
     4921 gttcgaggtg taacctactt ccccttccag caagcgctgg tagtcaataa agcggaagac
     4981 ccccgcctga acttcatagg aaagtatcgt cttactggtg ttcaccgagg agaggatctc
     5041 accggtactc acattgacga cacgcaggtt cacggcaatc tgatcaagct ggtattgcgt
     5101 gtctgcaccg atcccaaaat atcttgcccc aacaccgccc gatttaacgt tgctttcata
     5161 accgataatc gaaccttcaa ccatgatatt tgccgccgtt aaagattgca gcgggattcg
     5221 gttattaatg gcaactgtgc cgttttcttg tgccgcacga ataatcttgc gctcgttaag
     5281 cagattttgt aagccctggc gctccagcgg tataaaccag cgagaatctt tcagtgccgt
     5341 gaccagcatt gccgtggcgc tttgcggaac agcagtggag aagttacttg ccgggtaggg
     5401 tttaaattgt ccggtttcgt cctgaatgtt gtataccgaa acaaagatct tacccgtcgg
     5461 cgctggcaga tgggtcaaat ctttgtagct ctgagcacga ggcattaatg tcggtctggc
     5521 ggcttcttta ggcggggcgg ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa
     5581 taagcgctgc atgattattt tccttatgaa gctggggctt aaaaatcggt tgagttattt
     5641 tgtaaacccg aaacctggat ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac
     5701 tgcaattgac catcgcggtt agcaatatca acaatataat cgttggtcac catgcggccc
     5761 ggtttaccgg tattaatatt cgacagtagc ccacccaaaa tttgtgactg gatggcctga
     5821 gtaaagttat ctaacgctga tggcgtttca ataccaaagt catcgttata gctcggatct
     5881 ttataagagt tttgtgcctg agcgctattt aacaaaaaag cgccattatt tgggttacca
     5941 ccaaagtttg gattacggaa ctggaaagtc atggatccag cccaacttaa tggcgaaata
     6001 agcatgagta gaactactgc atgtttgaca cgcattgcag cctccgaaca atttattatt
     6061 taaaattcat catgcgccaa atcgcccgta cttaataacg cctggtttat ctggcgacga
     6121 tttagtgctt cttcagtttg aatcagtgca aagacgacag ttttctcgaa gtctcttttc
     6181 aacggaaata aaaaagtctg gaaaataacg tcctgattga ccgttatagt gatccaactt
     6241 ccccatcgtg cactgggcct ttcattaatc gttaagttac ccgtatagtc actttcccat
     6301 ttatcactaa aggctcggta aaaatcatgg ccaatagatg aaacagtatg gtcagttaac
     6361 aatcccggga cttctacctc aacggcatga agattccctg cggcgaacag aaattctgcc
     6421 gccacaatcc agcgtaaata acgtttcatg gctttatcgc ctgaggttat cgtttgccca
     6481 ggaaaccgct tgtgtccggt tttttacggc tatcttcttg aaaagattat aaagatgcgt
     6541 tttaaccgta ttttcgctga tgaacaacga acgagcgatc tcgttattag acgcgccgat
     6601 acgcagctta ttcaggatct ctttttcccg atgagtaagg agggctgatt ccgtgctgtt
     6661 ataacgatag ttacctgaat gcgtaatcag gtaactggca agcttttgcg taaagtagca
     6721 ttcgccgcgc aggacgcctt gcaacccatt gacaacacgt tcttgatcct ccatggcata
     6781 aaaaacgccg ttgatatgag gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt
     6841 atttagcaac aatattttga tattgttgtt tttcctgctc aaagtatcct gccaataatg
     6901 gataagcttt ttatccgctt ccatcatatc cagaagaata atagagcctg aagatatatc
     6961 gtccagagaa cgttgaatat tatgtaattt tcctgtaatc gccagcgatt gtttaaggtg
     7021 ctgcaagaga gctgtcgctt gcaaagaagg tttagtgatc aacaataatg tatgaccatg
     7081 aatactatgg acttcattaa acatgatgaa accccgcttt tttattgttc gcacacctga
     7141 cagctgcctc taaaatagaa gcaccagaag tactgacaga tgttgcactg ctgtgtgtag
     7201 taataaatca gccctaaatg ggtaaaatat aaaactaatg gattacatct gatttcaatc
     7261 ta
//
LOCUS       DAJOTB010000207         7159 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJOTB010000207 DAJOTB010000000
VERSION     DAJOTB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.207"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(657..929)
                     /locus_tag="OHN28_004458"
     CDS             complement(657..929)
                     /locus_tag="OHN28_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCQ2299730.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNG"
     gene            complement(1212..3899)
                     /locus_tag="OHN28_004459"
     CDS             complement(1212..3899)
                     /locus_tag="OHN28_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCQ2299731.1"
                     /translation="MKTPSVSTISGAAIGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEVAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKSLHGYSLPLLSQPLNLAGITFSTGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSEGWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKTLAEHLEQHVAH
                     YHGAPFRDWLYCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQSCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(3896..4306)
                     /locus_tag="OHN28_004460"
     CDS             complement(3896..4306)
                     /locus_tag="OHN28_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299732.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMVRVHNEPVYIEDGER
                     IELSHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(4299..4535)
                     /locus_tag="OHN28_004461"
     CDS             complement(4299..4535)
                     /locus_tag="OHN28_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299733.1"
                     /translation="MKSMNFKQGCILSEQGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGVMRDE"
     gene            complement(4532..5122)
                     /locus_tag="OHN28_004462"
     CDS             complement(4532..5122)
                     /locus_tag="OHN28_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCQ2299734.1"
                     /translation="MPALINPGNDQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCYL
                     KAILHALGVFFCVAALTHLKFMVWWLIVFFYFLRHIIRIMVVQAGQLSGWPVSFRAGI
                     PTPVWATTHERRNSGGSVTRYLKEVAIMATVPALSHPEFTFVFLAVRRTDRDARPRPV
                     RVIAECEHAARLKLATEFILSFAARIPVKRAGEVVA"
     gene            complement(5210..6214)
                     /locus_tag="OHN28_004463"
     CDS             complement(5210..6214)
                     /locus_tag="OHN28_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCQ2299735.1"
                     /translation="MYAFKTERPDAARRYHNKFVTGTNYQEESNRRFALSEPTENILR
                     TSILESGWLIKNITLRDVNAVSGNAVNIGASELYTGRVDGGRFHKKMAINGTEFFLTE
                     TDTCARISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEI
                     NKKGEDVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDALANHLITELIAEQFRE
                     DPRLVVLVGAELAAHQRLKLFNAADRPSDVNAAQMATSSVAGRFAFIPPFMPGKRLAV
                     TTLDNLHIYTQAITRWFRAEFDDESSEYVHSYLRNEGYALGEPELYAAVDECTLTFAD
                     "
     gene            complement(6237..7097)
                     /locus_tag="OHN28_004464"
     CDS             complement(6237..7097)
                     /locus_tag="OHN28_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299736.1"
                     /translation="MVDNVFKKKLASIKNEHVSVLDSYKVRSFKETHSDTACIVRIIE
                     IYSLNKLRAKGEKLYSLTGLTVPDTETVANEINLLLSRYAQLCRQEEEELSFRQREVT
                     NAEVAWKSTFSKNGVSSIAEAKTNKMGHAERADAERYYHLAVSRLNEQHSRLSTIKLL
                     PGVLADEGNYIGKGIDKRLLNIFPQSGQIPADFISVFNDSDVVRDIKFITDALKSLSD
                     SVSEIISRCSVPTDRYVLNNGGMARAMAYREYYRADNYVLRSVVSDRDYVEHVMKYNL
                     VTEYKNKIFS"
BASE COUNT         1816 a         1941 c         1491 g         1911 t
ORIGIN      
        1 gcaatttgat aagttatgac gaattctcac catccagtta ctacctgtac atactaatca
       61 aaaaaaacga gtacgcaaga tacgaaagta cttttcttgt tcaccagtta gcttaccttg
      121 atcctcacca gcgactggaa gataggtagc agtatatgtg gaaaccttcc cctagctagg
      181 tctacaaacg tagtttgggc atttaggctc cacgattcac ggtttgacac ggcctgatat
      241 ctgcacattc gttttgatat gcagcaatcg tgaaaaagga aaatatacgc tcgacagacc
      301 aaaacggcac tacaccgcac ccgcctgcgg ttttcatatc actaaaaatt ttcagcctgg
      361 attttttaca aacgataccg ccagtccgtg ccagtcctcg agatgtactg cgatacagga
      421 actgaaaaga atgaaaagaa attcattttt tttcagtttt actgtccgtc gaatctatgg
      481 cctgaatacg caactctatg atattaaatg gataattaat tttaggtgtg tgattttatg
      541 aaggtttgtg aaacatgaag aagataacca gttgaaaaat aataatttta tgaccgtata
      601 aactgaaatc ctgtgttcag gttccgggaa cagatcggcc attcccgacg catactttac
      661 ccattctttt gcgcagccag cagcaacttt aaagcctttt cccgttcctc gggtggaatt
      721 gcatcaatca gctctttaac ccttccctgc ccgttcagcg cgcttggact aacggaatgg
      781 gagaaagcaa cattcatcac aaacgagtga ccgcactcca cattagagca atagcagtac
      841 acatcgtaca gttgtgggtg tttcctgttt gttttaccta tccgtgctac tgcctgacat
      901 gtggggcagt acattctcat catcttcatc gttctgtgcc ctatactgtt gcccgccaga
      961 agaagggatt tggatcccta cctcttgttc tgaacgtgta acactggtaa cacgtgtaac
     1021 gctattgatt taaaaggatt taatctgtta ccagttagca aaatcaagag gtaacgcgtg
     1081 gtaacacacc attttttgtt acctctgtta ccagtcagta tttttgactg gtaacactga
     1141 agaaccgcat cagtaacggg tgttacctct gttaccagtg ttacctcata aaaataagac
     1201 tcacgcgata atcactcagg ttcagcgctt cccagtacct gtgtattgaa ctggtacaca
     1261 cgtttaagcc ctatttctgg caggcgaatg ctgttttgcg tccggccatc ttcaccgggt
     1321 ttcagccagc ccgcgtccac acacaacctg gccaccttac gcgagtcaaa tcccttacag
     1381 atctccttcc agcctgacgg gagaacgtag aacgtcacaa ccggctctgt cacgttgtct
     1441 cctttatcga cttttctgaa tcccatcatt gaaacaggcc ggttcctgtc gtcattccag
     1501 tcagcaaacc gactgaactg attacgcgtc atgaagtccc gaacctgttc aagtgccgct
     1561 ttatcttcct gattagctgt atggccccgg tcagccatcc atgcagccag acagctttga
     1621 gcggcacgaa aggcctctcc ctccggccat cctgtgatgc ccgcttttgt ggccagctcc
     1681 ccggccatcg caaccagagc aaaacgggta acagcccggc caacctgatt cccggcattt
     1741 tccggcgtca gccttcgggt atactctttc aataaggctt tcgcctgact ggtcagttcc
     1801 ggcagatcag cggtcaggca atacagccag tcgcggaatg gtgcaccatg ataatgcgct
     1861 acgtgctgct caagatgctc agccagagtt ttcccgctac tgaagccatg aagttcttca
     1921 aacacgccat acttgcccga atcgcttggg atctggatca ttctgacctc aaccccggca
     1981 tacgtacgtt ccccggcgct tgccgcatgc tctaccagtg acagttcccc ggtagacaga
     2041 aacaacagat tccagcggtt ggtttccctt accgaaccat ctgttctggc tctggctttg
     2101 ccctgcccgt tagccagcat ataagcgata tttcccgcct ccctgccgtc aacctcccgg
     2161 atttcatcca gcatcagcgt ggcatcattg cggcggctcg ccgttccttc taaggcattc
     2221 cccgtcgccc gccacgtatg ccagaaatct gttccaccac atactgatgc agccactttc
     2281 atcgtcgtgg ttttaccgtc tgtggattcc cctttgaggt gataacctcc accgccgata
     2341 ccaaccagtt tcagaagggg ggcagcaaat gccagactca ccgcaaaggc aagacgggca
     2401 ttctttatgc aataacggcc tatattttca cgccagccct ctgatgtgcc gctgacacga
     2461 aaatcacgcc cctgcacact cgatgtttgt aagatgactg actgggcttc gcgccctatc
     2521 acttcatcct ggagaacgta aaccccgccg tgccagccag ttttattcac acaggttact
     2581 tttctgtccg gtttacacag cgagatatat tccatcagga atgctctggc catgccgtta
     2641 atgttgatgt aagaaagccc gttaaccagc agaacgcggc gcagttcttc cccgctaccg
     2701 cccagcatct ccatcggcat tgcccattta cggctgtttc cgttggtatc ttcccattcc
     2761 agcaaacgcc cgtaattact tccatcagca tcactggtga ttgccgtcac ccgcaacgga
     2821 ctgcaaattt tgatattacg tatctctgta tcaccatcag acttattaac cagcttgtcg
     2881 taccacaaat attcttttgt cagccggaac ccttctggta aacgggtctg ccctttgcca
     2941 tgcagcgtca tttcttcacg aaaggcatct ctcgcccgtt ctttccccac ttcctgccga
     3001 taatcatccc agtcagcctt atggcgtacc ggagggagcg ttacccagcc atccaccgct
     3061 ttggcggctt tctctgcctg aatacgcccg gtgttttctt tgccgtcagc cagatcatta
     3121 tctccggcca gaatgatccg ggtttccggc cagcgttttc ggatctgctc cgtcaccttc
     3181 agtaaattgg tggcagcgac agccgccacg atccatcctt cagtcagcag actgacggta
     3241 agcgccgtgg caaaaccctc agtgataatc acctgttcag gtgtttcagc gggtatatcg
     3301 gtcagggcaa tgaatgcgcc agacagctgg ctaccgggca gcagactttt atccccgtca
     3361 ggattgataa gctggccacc agtaatattc ccggaaatat ccgtcaaagg cagcagtaat
     3421 gaaccggtgg aaaatgttat tccggccaga ttcaaaggct gtgacagcaa gggaagtgaa
     3481 tacccgtgta agctcttatt tgtcagatag accggctcac cattgcagga ctgctgtctg
     3541 agtctggtat atcgctccgc gcccgcttct tttcctgcgg cctttttcct ggctggcaat
     3601 gcgggcttct cctgtatttc tggtaatgca agcgcctcag ataccatccc ggcgacttct
     3661 tttatctttc ttcctgtcac cagacgaacc agatcaagac catcgccatg accgcactga
     3721 ttacaaaacc acgtccctcg ccccgcttta tcgtccagtc gaaaacgatc ttttccacca
     3781 caggcagggc aagccccatg atgtcctgcg gacggtactt taatacctaa tgctgacagg
     3841 atataaggcc agcgcccaat tgcagctccg gaaatcgtgg atacagacgg tgttttcaca
     3901 gcagcacctc ctgaccttcc ggcttcacaa attcaaaacc ttcgggtagt tcgccatcag
     3961 gtgctccgaa aataatatcc cggtaaacct cacgcatctc tgtgacacca aaaactgaca
     4021 ggcgaatatt ttctgacata aagcctggtt taagcatttt ttccagtttc tctgcggcca
     4081 gtatgcaacc agcatcagcc ccaaactcat tcacccacgg catttcaata tggtaaataa
     4141 tattactcac cactttttcg tgggagagtt ctattcgttc gccatcttca atataaacag
     4201 gttcattatg aacacgcacc attaaggaaa tgaatgcatc agcaatgtag tagcgtaaga
     4261 ctgctgttcg taaagcatgc gcctttaaat tttgattatt attcatcgcg catcactccc
     4321 ttaattgtgc tggtttcctt aacaacgcca tccagttgtt cggcaataca ggccactaaa
     4381 gccgcagcgc cttccgctga aacagttctc gctccattca ctgtgggcac ggacaagacc
     4441 tctgcaagaa actcactggc cagtgccgcc cgtaacagac gactctcccc ctgttcactc
     4501 aatatgcagc cttgcttaaa attcatggat ttcatgcaac cacctccccg gctcttttca
     4561 cagggatacg ggcagcaaag ctcaggataa attcagtggc aagtttaagg cgtgcagcat
     4621 gctcgcattc tgcgattaca cgcacagggc gtggacgggc atcacggtct gtacggcgga
     4681 cagccagaaa gacaaaggta aattcagggt gagataaagc agggactgta gccataatgg
     4741 caacctcctt cagatagcgg gtaacgctac caccggagtt cctacgctca tgggtggtag
     4801 cccagacggg ggtaggaata ccggctctga aggataccgg ccagcccgaa agctgccccg
     4861 cctggactac cataattctg atgatgtggc gtaaaaaata aaaaaataca atcagccacc
     4921 acaccataaa ttttaggtga gtcaaagctg cgacacaaaa aaacacgcct agcgcgtgta
     4981 gtatcgcctt cagataacac gggttcctac gcccggctgc cgattttgcg gcaaccttta
     5041 aactatatcc cgcgcattcc tggtgaggca agaaatttat aaccgaaaac tgatcattgc
     5101 ctggattaat taacgctggc atgcttaagc ctctgcttaa aaatagttct tcatttttcc
     5161 tgatgctgta aacgagaccc gcctctgagc gggtctttct gtcgctaagt caatccgcaa
     5221 acgtcagggt gcactcatca acagccgcgt acagttcagg ttcaccgagt gcataacctt
     5281 cattgcgcag gtaggaatgc acgtattcac tgctttcatc atcaaactca gcacgaaacc
     5341 agcgtgttat cgcctgagtg taaatatgca gattatccag cgtcgttact gccagacgct
     5401 tacccggcat aaacggggga ataaacgcaa agcggccagc aacagaactt gtagccatct
     5461 gtgccgcatt cacatcagac gggcggtctg cggcattaaa taacttcagg cgttgatgtg
     5521 ctgccagctc tgccccaacc agtacaacca gacgaggatc ttcacgaaac tgctcagcaa
     5581 taagctctgt aatcagatgg ttggccagag catcaatatt tttccaggtg ccagtctctc
     5641 ccagagtgac aggctcagag atgatttgct ttccgttccc gtaggctttc gccagtgcat
     5701 gccagccaat attgacatcc tcgccttttt tattgatttc cgggtttgta gtatttgcaa
     5761 tcgacacacc gttgaaaccg acccgcagca tgtccagcgc atatgcctgg gcaaaaaagc
     5821 tctcaaccgt tttctcaaac tcatccggca cgccggtatg gtaaatatga gacatatcgg
     5881 aatagctgat ccgggcgcag gtatccgtct ctgtcagaaa gaactccgta ccattgattg
     5941 ccattttttt gtggaaacgg ccaccatcca cacgtccggt atacagttca ctggcaccaa
     6001 tattgacggc attacctgac acggcgttaa catcacgtag ggtaatattt ttaataagcc
     6061 acccggattc cagaattgag gtacgcagta tattttcagt aggttcgctt aatgcaaagc
     6121 gtctgtttga ttcttcctga tagttagtgc cagtgacgaa tttattatga taacgacgtg
     6181 ctgcgtccgg cctttcagtt ttgaaggcat acatagtttt tcaccttttt aagtatttaa
     6241 gaaaagattt tatttttgta ctcagtaacc agattgtatt tcattacatg ctcaacataa
     6301 tcccggtcac ttacgactga acgtaataca taattatctg ctcgatagta ctccctgtaa
     6361 gccatagctc ttgccattcc accgttattt aatacataac gatcagttgg aacgctacaa
     6421 cgactaataa tttcactcac ggaatcagat aaagacttga gtgcatcagt aataaattta
     6481 atatcacgga caacgtcact gtcattaaat acagaaataa aatcagcagg gatctggccg
     6541 gattgaggaa atatattcag taaacgctta tcgattccct tcccaatgta gtttccttca
     6601 tcggccagta ctcccggcaa aagcttgatc gtgctcaatc gactatgttg ttcattcaac
     6661 cgggaaacag ccagatggta ataccgttca gcatccgctc gttcagcatg ccccatttta
     6721 ttcgttttgg cttcagcaat gctactgacg ccgttttttg agaatgtgct tttccatgca
     6781 acttccgcat ttgtcacttc acgctgccgg aaagataact cttcctcttc ctgacgacac
     6841 aactgggcat aacggctcag gagcaggttg atctcattcg ccacagtttc cgtatcaggt
     6901 actgttaacc ccgtaagtga atacagcttt tcacctttag ctcgcagctt attcagtgaa
     6961 tatatttcaa taatacgcac aatacaggcc gtgtcgctat gagtctcttt aaatgatctt
     7021 actttgtaac tatctaacac cgacacatgt tcgttcttaa ttgatgccaa tttcttctta
     7081 aaaacattgt caaccatata tttgccacct caatttagga gtttaatttt ctattgagaa
     7141 tattcttctc tatccattc
//
LOCUS       DAJOTB010000208         7117 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJOTB010000208 DAJOTB010000000
VERSION     DAJOTB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.208"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            76..1191
                     /locus_tag="OHN28_004465"
     CDS             76..1191
                     /locus_tag="OHN28_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2299737.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADQEGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1784..2635
                     /gene="hchA"
                     /locus_tag="OHN28_004466"
     CDS             1784..2635
                     /gene="hchA"
                     /locus_tag="OHN28_004466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCQ2299738.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2743..4101)
                     /gene="hprS"
                     /locus_tag="OHN28_004467"
     CDS             complement(2743..4101)
                     /gene="hprS"
                     /locus_tag="OHN28_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCQ2299739.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4101..4772)
                     /gene="hprR"
                     /locus_tag="OHN28_004468"
     CDS             complement(4101..4772)
                     /gene="hprR"
                     /locus_tag="OHN28_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCQ2299740.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4905..5318
                     /gene="hiuH"
                     /locus_tag="OHN28_004469"
     CDS             4905..5318
                     /gene="hiuH"
                     /locus_tag="OHN28_004469"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCQ2299741.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5427..6431
                     /gene="msrP"
                     /locus_tag="OHN28_004470"
     CDS             5427..6431
                     /gene="msrP"
                     /locus_tag="OHN28_004470"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCQ2299742.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6432..7067
                     /gene="msrQ"
                     /locus_tag="OHN28_004471"
     CDS             6432..7067
                     /gene="msrQ"
                     /locus_tag="OHN28_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCQ2299743.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
BASE COUNT         1986 a         1665 c         1511 g         1955 t
ORIGIN      
        1 atccaaatgt gctttttttg gataacagca cttattgata tattcatgaa gattataatc
       61 acaagggaat acataatgaa aagaaaagtt ctggcaatgc tggtcccggc gttattagtt
      121 gctggcgcag caaatgcggc tgaaatttat aataaaaatg gcaataaagt tgatttttac
      181 ggcaagatgg ttggcgaacg catctggtca aatactgatg ataataacag cgaaaacgaa
      241 gatacctcct atgctcgttt cggtgttaaa ggtgaaaccc aaatcaccag cgaactgact
      301 ggtttcggtc agtttgaata caacctcgac gccagcaagc ctgaaggtga aaaccaagaa
      361 aaaacccgtt taaccttcgc cggtttgaaa tataacgagt taggatcatt tgactatggc
      421 cgtaactacg gtgttgctta tgatgccgca gcttataccg atatgttagt tgaatggggt
      481 ggcgattcct gggcttccgc tgacaacttc atgaacggtc gtaccaacgg tgttgcaacc
      541 taccgtaact ctgatttctt tggcctggtt gatggtcttg attttgcgat tcagtaccaa
      601 ggcaaaaaca gcaaccgtag cactaagaaa caaaatggtg acggctatgc gttgtctgta
      661 gactataaca tcaatggttt tggtatcgtt ggtgcatata gcaaatctga tcgcaccaac
      721 gatcaagttg ccgatggaaa cggttcaaat gctgaactgt ggtcattggc tgcaaagtat
      781 gacgcgaaca atgtctacgc agctgtaatg tacggtgaaa ctcgtaatat gactccgggt
      841 tctattgata caggtgttgc cgatcaagaa ggcaatacaa taatgcgtga tcaacttatc
      901 aatgaaaccc agaactttga agcggttgtt cagtaccaat tcgactttgg tctgcgtccg
      961 tccttaggtt atgtgtactc taaaggtaaa gatatcaaag gtgtacctgg acatagatat
     1021 gttgacgctg accgtgtgaa ctacattgaa gttggtacct ggtactactt caataagaac
     1081 atgaacgttt atactgcata caaattcaac atgctggata aagatgacgc cgccattact
     1141 ggcgcagcag ctgatgacca gtttgcagtt ggtatcgtct accagttcta atcagccaac
     1201 gcgccgaggc gtgttactgt aaaacataac accagcctga gttcgtccct catcgaactc
     1261 aggctttttt attggcattt catctccatc tgcggcacac gcaattgcca aagcatatcg
     1321 atacagcaca ttaccccgcc cctcctggct caataaaatc taccagcgtc tcttccagcc
     1381 aacacccaaa tgcatttctt tatgaattaa tccgcaacaa aaccgcttcc catatagaaa
     1441 tatttgcaaa aatagtgcat gaaaaaaact ttttccctac caatccggga tataaatatt
     1501 catcagtacc acaacattcc acagcactaa atctctcccc atcgccccca aacctctgat
     1561 aatggtctaa aatcattgag gccacttgcg acgacgttac tcaataagga aagtttggcg
     1621 tgaataatat tcaacaccag tgcagcatca tcattacata cttatgcccg ccaactaccg
     1681 ctttcgatat attgactcaa tttttcagcc tggttaaaaa tagcgaccag ctcagtcgca
     1741 aatatagtga ctaccctaac taagcaacaa taaggaatac actatgactg ttcaaacaag
     1801 taaaaacccg caggtcgata ttgctgaaga taatgcgttc ttcccttcag aatattcgct
     1861 tagccaatat acgagtcccg tctctgatct tgatggcgtg gactatccaa aaccgtatcg
     1921 cggtaaacat aaaattctgg tgatcgccgc ggacgaacgt tatttgccga ccgataacgg
     1981 aaaactgttc tcgaccggta accatccgat tgaaacgttg ctgccgttgt atcatctcca
     2041 tgctgcaggt ttcgaattcg aagtggcgac catttccggt ctgatgacca agtttgaata
     2101 ctgggctatg ccgcacaaag atgaaaaagt aatgccattc tttgaacagc ataaatcgtt
     2161 attccgtaat ccgaagaaac tcgcggatgt tgtcgccagc ctcaacgctg atagcgaata
     2221 tgcagcaatc tttgttcctg gtgggcatgg cgcacttatt ggtttaccag aaagccagga
     2281 cgtggctgcc gctttacagt gggcaatcaa aaatgaccgt tttgttatct ccctttgcca
     2341 cggtccggca gcttttctgg cacttcgcca cagcgataac ccactgaatg gttattctat
     2401 ttgcgcattc ccagacgccg cagacaaaca aacgccagat attggctata tgccgggtca
     2461 tctcacctgg tacttcggcg aagagttgaa gaaaatgggc atgaacatca ttaatgacga
     2521 catcaccggg cgagtacata aggaccgtaa acttctcacc ggcgacagtc cttttgcagc
     2581 gaatgcgttg ggtaaactgg cggcgcagga aatgctggca gcttacgcga gttaatccct
     2641 gttacattgt aatgtaagcg cataatcaat cgcagccacg ttaatactca cgacgtggct
     2701 gcgttttttc acggttaact tatggcgtac tgaagcccga tgttaatttc tttgcggtaa
     2761 cgtaattcgg aacacgttat gcttactgag atagtgatac gtagcacttc ctccatgtaa
     2821 ttcggcaatc gctttgacta aagaaaggcc aagtccctga cctacggaat gacgcgaatt
     2881 atctccccgc caaaatctac ggaagagttt ttcaggctca ttaattttcg ttccaggact
     2941 ggcgatatca atattaagag aaccgttggc atcaagaaaa ctggttatat gaatacgcga
     3001 tttttctggt gaatatcgaa tggcgttaac aataagattc gataacattc gttgcagtaa
     3061 aattttatcc gcaaagattt gttgattgca cttgacctta aagcaaatct ctttctcgtc
     3121 tgaaagatat tcaagatagt ccaaaagatt ttcaacttcc ttattgagag aaagcgaatc
     3181 cagtttcacc aaaacattgt ttttgtctgc cctggcaaga aacagtatat tctctgttaa
     3241 ccgcgaaata ttttccagct cttcgatgtt tccggcaatt gttttttgat attcagcgat
     3301 acttctggtt tgactgagcg taacctgatt ctgacccagt aatgcattaa ttggcgttct
     3361 aagttcatga gcgagatcgt cagcaaattg actaaggcgc tcaaaatctt tgactaaagc
     3421 atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta atgcgttaac
     3481 ctcaaccggc actcggctat cgttataatt cagcgcttcc gttacgccac tcaacttttt
     3541 gatctctcgc aaccccgttc tgattaacaa cggacttaat aatgagcaca atataatgac
     3601 gacaatgcaa attataatgc tattaatttt atactgttca agcatattat gtctggctga
     3661 agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatgt taattcgtaa
     3721 agcatctatc tctgtatcat taataatgct tctgtaaatg cctgctgcgc tgattgtctc
     3781 actagcaggt atgttattta acatgtcatc actgacattt gtccggttaa caattttatt
     3841 gatgccatca ccatgaatga tcaagatatc ctgactaaca tccatcatcc ggttaaaata
     3901 gacaggtaat gtatctggat ttaccccatc aattaacaac tgcttgatct gcgctgtccg
     3961 gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat tatagagagt
     4021 ccatacaatt ccagcgccag caacagacag cagaaatata aaaagcaagg ttaaacggac
     4081 ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc catcccccgg
     4141 attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag cctgcgaatg
     4201 gcgacatcca ccgtattggt atcactatca aagttgattc cccaaatttc actcgcaata
     4261 accgttctgg gtataatctc gccagctctg gaggccagta gccaaagtaa ctgaaactcc
     4321 ttgcgcgtca gtgtaatact gatattgtcc ctgctcacac tttgactgac agagtccatt
     4381 tttaagccac tgatttctaa tgttgaattc aaagcgtgat gttgccttaa ttgtgcccga
     4441 acccttgcca gcaactcaga aaatgaaaaa ggttttacca gataatcatt tgctccactg
     4501 tccagccctc tgactctgtc atcgacagaa tcccttgctg taaggcaaat aacaggagtt
     4561 tgctttgccg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg aagcataata
     4621 tccagaatga tcaatgcata atcatccttc agcgcaagat aaagcccatc tctgccatca
     4681 gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca ttcctgggtc
     4741 ctttgattat cttcaataag tagaatcttc atgaaaataa tatgccataa atcatatgtt
     4801 atagaaacaa cctggttcat tacaaaattg taatgctgct gtaaggttac cctggacgtt
     4861 ttttcgctat cctcaaaact cattcacatg acaaggatat aaacatgtta aagcgttatt
     4921 tagtactctc ggtagcaacg gcagcatttt cattaccttc tttggtttat gccgcacaac
     4981 aaaacattct tagcgtgcac attttgaacc agcaaacagg aaaacccgct gccgacgtga
     5041 cagtcactct tgaaaagaaa gcggataacg gctggttaca acttaatacc gccaaaacag
     5101 ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcgactact ggcgattacc
     5161 gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt ttcttccctg
     5221 agatccccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg cctttgttac
     5281 tcagccaata tgggtattca acctatcgtg gcagttaatg cgtaaatatg taaaagctga
     5341 tctctgctac tgttagggag gtgactccct gacagattaa cagtaaacgg ctcttgttgg
     5401 ctaacgacaa aaaagtgtga tggcttatga aaaagaatca atttttaaaa gaatcagatg
     5461 ttacggccga gtcggtattc tttatgaagc gtcggcaggt gttaaaagca ctgggcatca
     5521 gcgcagctgc attttctttg cctcacgctg cgcatgccga tctgcttagc tggtttaaag
     5581 ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga gttcagcaag cctgccgcct
     5641 ggcaaaataa cctgccactg acgccagcag ataaagtctc cggttataac aacttctatg
     5701 aattcgggct ggataaagcc gatcccgctg ctaatgctgg tagcctgaaa accgatccat
     5761 ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcat gatgatttaa
     5821 cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa gcgtggtcga
     5881 tggtggtgcc gtggattggt tttccgctac acaaattgct ggcgcttgcc gaacccacca
     5941 gcaatgcgaa atatgtcgct ttcgaaacga tttatgcgcc ggaacagatg ccaggccagc
     6001 aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg cgtctcgacg
     6061 aagcaatgca tccgctcaca ctgatgaccg taggtgttta tggcaaggcg ttaccaccgc
     6121 aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa gggattaaat
     6181 cgatcgtcag tattaagctg acccgcgagc gtccgccaac cacctggaat ctggcagcgc
     6241 ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg cgctggtcac
     6301 aggctaccga acgatttatt ggttcaggcg gcattctcga tgttcagcgc cagccaacac
     6361 tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg gatttgcggg
     6421 agaatttcta aatgcgcctg acagcaaaac aggtgatatg gctgaaagtt tgcctgcatc
     6481 ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt ggactaggtg
     6541 ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa tttttgcttg
     6601 cggccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg atacgcactc
     6661 gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc agttacgcat
     6721 tgctggagtt gggcgtgaat aatctggcgt tattaggtaa ggagttaatt acccggcctt
     6781 atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttc acttcaaccc
     6841 agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt gcataacttc gtctatcttg
     6901 tcgcgatcct gaccccaata cattatctgt ggtcagtgaa gattatttca ccgcagccac
     6961 tcatctacgc tgggctggct gtactgcttt tagccttacg gtataagaag ttgctttccc
     7021 ttttcaaccg gttacgcaag caggcccaca acaaattatc tctataactt catattgccg
     7081 acaaacttcg cgttatcccg gccggatgcg gcgcgag
//
LOCUS       DAJOTB010000209         7065 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJOTB010000209 DAJOTB010000000
VERSION     DAJOTB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.209"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            570..1559
                     /gene="araF"
                     /locus_tag="OHN28_004472"
     CDS             570..1559
                     /gene="araF"
                     /locus_tag="OHN28_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCQ2299744.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1629..3143
                     /gene="araG"
                     /locus_tag="OHN28_004473"
     CDS             1629..3143
                     /gene="araG"
                     /locus_tag="OHN28_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCQ2299745.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTNMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3158..4144
                     /gene="araH"
                     /locus_tag="OHN28_004474"
     CDS             3158..4144
                     /gene="araH"
                     /locus_tag="OHN28_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCQ2299746.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4311..5111
                     /gene="otsB"
                     /locus_tag="OHN28_004475"
     CDS             4311..5111
                     /gene="otsB"
                     /locus_tag="OHN28_004475"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCQ2299747.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5086..6510
                     /gene="otsA"
                     /locus_tag="OHN28_004476"
     CDS             5086..6510
                     /gene="otsA"
                     /locus_tag="OHN28_004476"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCQ2299748.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6517..6945)
                     /gene="uspC"
                     /locus_tag="OHN28_004477"
     CDS             complement(6517..6945)
                     /gene="uspC"
                     /locus_tag="OHN28_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCQ2299749.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
BASE COUNT         1770 a         1641 c         1840 g         1814 t
ORIGIN      
        1 attttaatga tggtgccttt tgtatcttat tgatattaat cgatgaattt gaattcatga
       61 tgaggcaatg attttataaa acgttttttc atttttgcga tatggctctc attatacgtg
      121 ttctgttaat taatgagcac agtgataatt aatggcgaaa tgaatgacgc gcattttcca
      181 catctttaag ttgcggttat tacaccattg caaaaactca acgccaggta atgaggccta
      241 ttgactggtt aaaaagaaga catcccgcat gggtaccaaa gacaacaagg acttccaggc
      301 taatcttatg gattattctg ctgtgcactc gataatttgt ctgacaaatt cgcttttcct
      361 tatgtctttt cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc
      421 tctcctatgg agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag
      481 ttaaaaggta atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac
      541 acaaaacgac actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca
      601 ttggtctggc agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggtttcc
      661 tggtgaagca accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg
      721 ggaaggattt agggtttgaa gttattaaga ttgccgtgcc ggatggcgaa aaaacattga
      781 acgctatcga cagcctggcc gccagtggcg cgaaaggttt cgttatttgt actccggacc
      841 cgaaactcgg ctctgccatc gtggcgaaag cgcgtggcta cgatatgaaa gtcattgccg
      901 tggatgacca gtttgttaac gccaaaggta agccaatgga taccgttccg ttggtgatga
      961 tggctgcgac caaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac
     1021 gtggctggga tgtcaaagaa agcgcggtga tggcgattac ctccaacgaa ctggatacgg
     1081 ctcgtcgccg tactacgggg tctatggatg cgctgaaagc tgcaggattc ccggaaaaac
     1141 aaatttatca ggtaccgacc aaatctaacg acatcccggg ggcatttgac gctgccaact
     1201 caatgctggt tcaacatccg gaagttaaac attggctgat tgtcggtatg aacgacagca
     1261 ccgtgctggg cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg
     1321 gtatcggtat taacggtgtg gatgcggtga gtgaactgtc taaggcgcag gcaaccggct
     1381 tctacggttc cctgctgcca agcccggacg tacatggtta taagtccagc gaaatgcttt
     1441 acaactgggt tgcaaaaggt gttgaaccga ccaaattcac cgaagttacc gacgtggtgc
     1501 tgatcacgcg tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat
     1561 ttgccggaaa aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg
     1621 gagacgttat gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc
     1681 ccggcgttaa ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt
     1741 tgatgggtga aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggaaactatg
     1801 cgccaaccac gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg
     1861 cagcacttaa cgcgggtgtg gcgattattt atcaggaact gcatctcgtg ccggaaatga
     1921 ccgtcgcgga aaatatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgtt
     1981 cattgctgaa ttatgaggca ggtttacaac ttaaacatct tggtatggat attgacccgg
     2041 acacgccgct gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaagcgc
     2101 tggcgcgtaa cgccaaaatt atcgcctttg atgagccaac cagctccctc tctgcccgcg
     2161 aaatcgacaa tcttttccgc gttattcgtg aattgcgaaa agaggggcgg gtgatcttat
     2221 acgtttctca ccgtatggaa gaaatatttg ccctcagcga tgccatcacc gtctttaaag
     2281 atgggcgtta tgtcaaaact tttaccaata tgcagcaggt tgaccacgac gcactggtgc
     2341 aggcgatggt cgggcgagac attggcgata tctacggctg gcaaccgcgt agttatggcg
     2401 aggagcggct gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg
     2461 cggttcgcag tggagaaatt gtcggtctgt ttggtctggt cggggcaggg cgtagcgaat
     2521 taatgaaagg catgtttggc gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac
     2581 agccgatcga tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg
     2641 aagatcgcaa agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca
     2701 gtgccagacg taaacatgtg cttggcggtt gtgtaatcaa caacggttgg gaagaaaaca
     2761 atgccgatca ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatca
     2821 tgaatctctc aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga
     2881 tgaaggtcat tttgctggat gaacctacgc gtggcattga tgttggtgct aagcatgaaa
     2941 tttacaacgt gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg
     3001 acttacctga agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggcgaaatcg
     3061 ccggtgaatt gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta
     3121 aagtcagcca ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg
     3181 ttctggcgca cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct
     3241 ggtggtgttt gcggtgcttt ttatcgcctg tgccattttt gtcccaaatt ttgccacctt
     3301 cattaatatg aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cttgtggcat
     3361 gttgttctgc cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc
     3421 gggtgtcacc acggcggtgg ttattaatct gactgaaagc ctgtggattg gcgtggcagc
     3481 ggggttgctg ctgggcattc tctgtggtct ggtcaatggc tttgttatcg ccaaactcaa
     3541 aataaatgcc ctgatcacga cactggcaac gatgcagatt gttcgaggtc tggcgtacat
     3601 catttcagac ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc
     3661 caactggttc ggtctgcctg cgccaatctg gctcaccgtc gcgtgtttga tcatctttgg
     3721 cttgctgctg aataaaacca cctttggtcg taataccctg gcgattggcg ggaacgaaga
     3781 ggctgcgcgt ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc
     3841 tggcctggta tcagcgatag ccggaattat tctggcttca cgtatgacca gtgggcagcc
     3901 aatgacgtcg attggttatg agctgattgt gatctccgcc tgcgttttag gtggcgtttc
     3961 tctgaaaggc ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac
     4021 cgtggagaac gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg
     4081 cttaatcctg ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt
     4141 ctgatgcttt tttctgcaac aatttagcgc ttttttcctc cacagccagc cgccacaacg
     4201 gttggctgtt cttcgttaca aatggcgacc cccgtcacac tgtctatact tacatgtctg
     4261 taaagcgcgt tctgcgcaac acaataagaa aagagaagga ggagaaccgg gtgacagaac
     4321 cgttaaccga aacccctgaa ctatccgcga aatatgcctg gttttttgat ctcgatggaa
     4381 cgctggcgga aatcaaaccg catcccgatc aggtcgtcgt gcctgacact attctgcaag
     4441 gacttcagct actggcaacc gcaagtgatg gtgcattggc attgatttca gggcgctcaa
     4501 tggtggagct tgacgcactg gcaaaacctt atcgcttccc attagcgggc gtgcatgggg
     4561 cggagcgccg tgacatcaat ggtaaaacgc atatcgttca tctgccggat gcgattgcgc
     4621 gtgatattag cgtgcaactg catacggtca tcgcccagta tcccggcgcg gagctggagg
     4681 cgaaagggat ggcttttgcg ctgcattatc gccaggctcc gcagcatgaa gacgcattaa
     4741 tgacattagc gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt
     4801 gtgttgtcga gatcaaaccg aaaggtacca gtaaaggtga ggcgattact gcctttatgc
     4861 aggaagctcc ctttatcggg cgaacgcccg tatttctggg cgatgatcta accgatgaat
     4921 ctggcttcgc agtcgttaac cggctgggcg gaatgtcagt aaaaattggc acaggtgcaa
     4981 ctcaggcatc atggcgactg gcgggcgtgc cggatgtctg gagctggctt gaaatgataa
     5041 ccaccgcatt acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc
     5101 gtagtatcta accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc
     5161 gttggcatac tgggggcact gaaagccgca ggtggactgt ggtttggctg gagcggtgaa
     5221 acagggaatg aggatcagcc gctaaaaaag gtgaaaaagg gtaacattac gtgggcctct
     5281 tttaacctca gcgaacagga ccttgacgaa tactacaaca aattctccaa tgccgttctc
     5341 tggcccgctt ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggatggc
     5401 tatctacgcg taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac
     5461 attatctgga tccacgatta tcacctgttg ccctttgcgc atgaattacg caaacgggga
     5521 gtgaataatc gcattggttt ctttctgcat attcctttcc cgacgccgga aatcttcaac
     5581 gcgctgccga catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc
     5641 cagacggaga acgatcgtct ggcgttcctg gattgccttt ctaacctgac ccgcgtcacg
     5701 acacgtagcg caaaaagcca tacggcctgc ggcaaagcgt ttcgaacgga agtttacccg
     5761 atcggcattg agccgaaaga aatagcgaaa caggctgccg gtccactgcc gccaaaactg
     5821 gcgcaactca aagcagaact gaaaaacgta caaaatatct tttctgtcga acgactggat
     5881 tattccaaag gtttgccgga gcgttttctc gcctatgaag cgttactgga aaaatatccg
     5941 caacatcacg gtaaaattcg ttatacccag atcgcgccaa cgtctcgtgg tgatgtgcaa
     6001 gcctatcagg atattcgtca tcagctcgaa aatgaagccg gacgaattaa tggtaaatat
     6061 gggcaattag gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg
     6121 atgaaaatat tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac
     6181 ctggtggcaa aagagtatgt tgccgctcag gacccagcca acccgggcgt tcttgttctt
     6241 tcgcaatttg cgggagcggc aaacgagtta acgtcggcgt taattgttaa tccctacgat
     6301 cgtgacgaag ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt
     6361 tcccgtcatg cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag
     6421 tgcttcatta gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat
     6481 aaagttgcta cctttcctaa gcttgcgtag gtgcggttaa cctcccgtaa gtggaactaa
     6541 cagcacatcg acctcacttg aggtaatcac tcttttcgcc gagcaggatg ctcgcgaaaa
     6601 gaaactgtga ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgc gacaaacttc
     6661 cagaatatgt tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc
     6721 atcctgaatt aacttatcaa gaaagctttg cgtttcttcc tgcatcacgt tgcgtaaatc
     6781 ttcaagcatc ggcgcagcta attgattgta catttccggg tcggaagcga gagtaattaa
     6841 actgatgtgt ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact
     6901 ttccggtgta accgcaacgg cgacaagaat attgctatag ctcataacct gttccttatt
     6961 ctgtgaactt caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac
     7021 tcagttaaac aggtaaatat tagtctggtc attagtaaat acaat
//
LOCUS       DAJOTB010000210         7030 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJOTB010000210 DAJOTB010000000
VERSION     DAJOTB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.210"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            115..828
                     /locus_tag="OHN28_004478"
     CDS             115..828
                     /locus_tag="OHN28_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2299750.1"
                     /translation="MEFKLPGFKNKKDVTDSSVSFEEKNIALQERMNRIYKFGGIGGM
                     LIGGLSLLALNAALPLKTTVVDAYLIDKVTGVAERLTSVKKENLSENEAIARYFITQY
                     IKHREGYSFFSLQHDYDYVMAYSAENVAADYNALFNSEQAPKLVYNKAEKTAMVQDNP
                     SVIISPSSRADDKDIGAYIRFRLTIRDVATGQTRQEFWNVRLTYRIEPQVEMVSGERN
                     NNPLKFVVTSYVRDKEARG"
     gene            833..1762
                     /locus_tag="OHN28_004479"
     CDS             833..1762
                     /locus_tag="OHN28_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HCQ2299751.1"
                     /translation="MKMNKGALIMTLLIAAHVCHAAVLPSGSRFDPRNQIVSYNPNNT
                     TIINSAVGYTTTLVFDEDETVISARTGFPQGWAVNKEDNLVYLEVRPVKQTVQKNNMD
                     ENGNTSSESVSVALDPENELERWRTNLFVRTTKRNYSMELNARTFRQPEKIAFVVNYQ
                     YPQERRKEQAEIEKKRTEALARRLEEQAINRSLENAKSPRNWQYWKRVAEGSQDISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMNGMQETLTNPVIKQSGSFTAVGVPVDKRFVLRL
                     GEQVVGIENQGFGKVRLPAGDTVSPDVKKEVIQ"
     gene            1759..2964
                     /locus_tag="OHN28_004480"
     CDS             1759..2964
                     /locus_tag="OHN28_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HCQ2299752.1"
                     /translation="MTEQENKIPTATEIEQQLRERRQKELEQAGKTPEEEPGKPALQL
                     GIEKLKKSRKGMIILVVGFLLLAAGVSVYYIPSIIRSVSSGDEKPASQPLATGAAKRQ
                     TGLSEDIDPFNTAQKKTEKPEEEKVISSEKTEPPENKQQSFSRALDVSLDGSQAGNSS
                     SSAGTSVSHTAASEPESDKKDEAKATAQTTESAPLAKITKLPYDPNLFIPEGTSIPCS
                     LDRRFVSDLAGKLECTVNSDIYSASGNVKLIERGTAAKLMYKAGSLNHGQGRVFVMAY
                     KLRTRSKPFIDIPLVDSQAAGALGEAGASGWIDTHFSERFLGAMMVGMIPDLSQAASG
                     IAQNNRDSQTDYTANSRQAFADIAREAFSNSVNIPPTLYKNQGEIITLIVGQDLDFSG
                     IYKLKMKGG"
     gene            2966..3997
                     /gene="virB11"
                     /locus_tag="OHN28_004481"
     CDS             2966..3997
                     /gene="virB11"
                     /locus_tag="OHN28_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HCQ2299753.1"
                     /translation="MNNENRHLIYDVVNDYFYHWLNEIEGVTEIAVNRPGEIFIKVRG
                     KWQWYEQKMSYSDCLSFASTLADFHDGGSVTPEYPLRSATLPGGERVQVVIPPATEKD
                     TVSITIRKPSGIFISHDKFIKQGFYSRVGGLSGDSVMEDNISALITSGYFERVIPESL
                     RQGKTIVFCGGTGSGKTTFANACLEYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPAE
                     GESKVITSASLLRSCFRMNPDRILMTEIRGAEAWDFLKASSSGHAGNITTVHESSPEY
                     AVLGIVQRCYMNPECQNLPFNVILRRVLSNIDIIMSIKYLDDEDFRFASGIYYRQLHF
                     DDYFRKLKE"
     gene            4000..5835
                     /locus_tag="OHN28_004482"
     CDS             4000..5835
                     /locus_tag="OHN28_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCQ2299754.1"
                     /translation="MSLKLPDKGQCFFIGLVMCLVTYYAGSVAVYFFNGKTPLYIWKN
                     FDSMLLWKIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKMDVALYGDAKF
                     ASDSDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAES
                     GAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREELEETNGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKISEKLGYITTTSKSTSKSRG
                     RSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPYFMDRLM
                     KVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL"
     gene            5832..6245
                     /locus_tag="OHN28_004483"
     CDS             5832..6245
                     /locus_tag="OHN28_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCQ2299755.1"
                     /translation="MKKILMISILFLTACSSPPEPPQVEWEKRPEVMNTQIMNWKPTS
                     GVIKSDNINSSWSKVLPGFKPENRLYDDSVFYAVAHSEKIVVRTSSFDSYWSAKYWLR
                     KNGATGVIEYQPLKRWLNSDYVEIYLSRINVQRLP"
     gene            6242..6544
                     /locus_tag="OHN28_004484"
     CDS             6242..6544
                     /locus_tag="OHN28_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCQ2299756.1"
                     /translation="MKKICKSLIVIFTVSWLTAPAYASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGNIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGKIKG"
     gene            6550..6807
                     /locus_tag="OHN28_004485"
     CDS             6550..6807
                     /locus_tag="OHN28_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299757.1"
                     /translation="MKKKEKIDFLNAIKAGHSGFIAGNPAHALDDKTMNAIIHNYEAT
                     IAPGKAGNCKETPLTKYCSRTMPERISVEEINEVKIYPELF"
BASE COUNT         2092 a         1349 c         1653 g         1936 t
ORIGIN      
        1 aactacttat tgtggccttc gtgacgcttt gtctcgctgg ttgtcaggcg tcacataaac
       61 taccgcccgt ttccgggaaa agcgaacctg ttaattctgc tgaggtaatg caaaatggaa
      121 tttaaacttc ccggatttaa aaataaaaaa gacgttactg actcatcagt ttcgtttgaa
      181 gaaaaaaaca ttgcactaca ggagagaatg aatcgtatct ataaattcgg tggtatcgga
      241 ggcatgttaa ttggtgggct gtctttactt gcattaaatg cagcattacc actgaaaaca
      301 acagttgttg atgcctatct tatcgataag gttacaggtg tggctgagcg tctgacttcc
      361 gttaaaaaag aaaatctttc tgaaaacgaa gccattgccc gatattttat cacccagtat
      421 ataaaacatc gtgaaggtta tagttttttc agtctccagc atgattatga ttatgtaatg
      481 gcttacagcg cggaaaatgt cgcggcagat tataacgcat tatttaacag tgaacaggca
      541 ccaaaacttg tttataacaa agcagaaaaa acggcaatgg ttcaggataa tccatctgtc
      601 ataatttcac cctcgtcacg ggcagatgat aaagatatcg gtgcgtatat tcgttttcgt
      661 ctgaccatca gggatgttgc taccggacaa acccgccagg agttctggaa tgttcgcctg
      721 acttatcgta tcgaaccgca ggttgaaatg gtgtccgggg aacgtaataa caatcctctt
      781 aagttcgttg taacaagcta cgttcgcgat aaagaagcca gaggttaata atatgaaaat
      841 gaataaagga gcgttaatta tgacgcttct gatagcggcg cacgtctgtc atgctgctgt
      901 tcttccttca ggcagtcgct ttgacccacg caatcagatt gtcagttata accccaataa
      961 taccaccata attaacagtg ccgttggata caccaccaca ctggtatttg atgaagatga
     1021 aacagttatc agtgccagaa ctggttttcc gcagggatgg gcggttaata aagaagataa
     1081 cctggtatac ctggaagttc gtcctgttaa acagactgtt cagaaaaata atatggatga
     1141 aaacggtaat acctcttctg aatccgtcag tgttgctctt gacccggaaa atgaacttga
     1201 acgctggaga acgaatctgt ttgttcgcac cacgaaacgt aattacagca tggagctgaa
     1261 cgccagaacg ttccgacagc cggagaaaat tgcgtttgtg gtgaattacc agtatccgca
     1321 ggaacgccgg aaggaacagg ccgaaataga gaagaaacga acagaggctc ttgccagacg
     1381 cctggaagaa caggcaatca accgttccct ggaaaatgcg aaatcgcccc gtaactggca
     1441 gtactggaag cgggttgctg aaggcagcca ggatatcagc cctgattatg catatgacga
     1501 tggtcgttat acctggttcg gcttcagtcc gttaaagaaa attcccagcg tctttgtgat
     1561 gaacggtatg caggagactc ttaccaatcc tgtgattaaa cagagcggga gttttacggc
     1621 tgttggcgta ccggttgata agcgttttgt tttacgtctt ggtgagcagg tggtggggat
     1681 tgagaaccag ggcttcggaa aagtacgttt accagccgga gatacggtat ccccggatgt
     1741 taagaaagag gtgatccagt gactgaacag gaaaataaaa tcccgactgc aaccgaaatt
     1801 gaacagcagc tacgggaacg cagacagaaa gaactggaac aggccgggaa gactccggaa
     1861 gaagagcctg gcaagccagc attgcagctt ggtattgaaa aacttaaaaa gtcacgtaaa
     1921 gggatgatta ttctcgtcgt tggttttctt ctgcttgctg ccggtgtttc tgtttattat
     1981 atcccgtcca ttatccgatc tgtgtcgtca ggggatgaga aacccgcaag tcagccgctt
     2041 gcgaccggag cggctaaacg tcagaccgga ctgagcgaag atatcgatcc ttttaatacc
     2101 gcacagaaaa aaacagagaa accagaggaa gaaaaagtca tttcttctga aaagactgaa
     2161 ccgccggaaa ataaacagca gagcttcagc cgtgcacttg acgtttctct tgatggcagc
     2221 caggcaggaa acagcagcag ttcagcggga acgtcagttt cacatactgc ggccagtgag
     2281 ccagaaagcg ataaaaagga tgaagcaaaa gcaaccgcac agactacaga atctgcgcca
     2341 ctggcgaaaa ttacgaaact tccatatgac ccaaatttgt ttatccctga gggaacatcg
     2401 attccctgct cactggacag gcgttttgtt tctgacctgg cggggaaact ggaatgtacg
     2461 gtcaacagcg atatatacag cgccagtggc aatgtaaaac ttatcgaaag aggaaccgcc
     2521 gcaaaactga tgtataaggc cgggtcttta aatcatggac aagggcgtgt gtttgttatg
     2581 gcttataagc tacgtacccg cagtaagcct tttattgata ttcccctggt tgactcacag
     2641 gcggctggcg cgttaggcga agccggtgct tctggctgga ttgacactca tttcagtgag
     2701 cgttttcttg gtgcaatgat ggtcgggatg ataccggatt taagtcaggc ggccagtggt
     2761 attgctcaga ataacaggga cagtcagacg gactatacgg cgaacagccg ccaggctttt
     2821 gctgatatag cacgcgaagc attttctaat agtgtgaata tcccgccaac gctttataaa
     2881 aatcagggag aaattattac tctgattgtc ggtcaggatc tggatttttc aggcatttat
     2941 aaactgaaaa tgaaaggggg ttaatgtgaa taacgaaaac agacatctga tttatgatgt
     3001 ggtcaacgac tatttttatc actggctgaa tgagattgag ggtgtcacgg aaatcgctgt
     3061 taaccgacca ggagaaatat ttataaaggt caggggaaag tggcaatggt atgaacaaaa
     3121 gatgagttac agtgattgtc tttcttttgc atccacactg gccgattttc atgacggcgg
     3181 ttctgtgact cctgaatatc ccctgcgttc agccacgctt ccgggtggag aacgtgttca
     3241 ggttgtgatc cctccggcaa cagaaaaaga tactgtttct ataacaatcc gtaagccgtc
     3301 aggtattttt atcagtcatg acaaatttat aaaacagggg ttttattcac gcgtcggtgg
     3361 tttaagtggt gattccgtta tggaagataa tatttctgct ttaatcactt ccggatattt
     3421 tgaacgggtt atacctgaat cactgcgtca gggaaaaacg atagttttct gtggcgggac
     3481 ggggtcaggt aaaactacct ttgcaaatgc ctgtctggaa tatataccgc atcatctgcg
     3541 gtgtatttct attgaagata ctgatgaggc aaaattcaga ttccataaaa accatgtaaa
     3601 actttactat ccggcagagg gggagagtaa ggttattacc tcagcgagtc ttctgcgttc
     3661 ctgttttcgt atgaatccgg acaggattct gatgacagaa atcaggggag ctgaggcatg
     3721 ggattttctg aaagcatcga gttcaggcca tgcaggaaac attaccaccg ttcacgaaag
     3781 tagtcctgaa tatgccgtgc ttgggattgt ccagcgatgt tatatgaatc ctgaatgtca
     3841 gaatctacca ttcaatgtca ttttaagacg tgtactgagt aatattgata ttatcatgag
     3901 tattaaatac cttgatgatg aagattttcg gttcgcttcc ggcatttact acagacaact
     3961 tcattttgat gattatttca gaaaactgaa ggagtgatta tgtctttaaa acttcccgat
     4021 aaaggccagt gtttttttat cggtctggtt atgtgtctcg tgacctatta tgctggttct
     4081 gttgctgttt atttctttaa cggaaaaaca ccactgtata tatggaaaaa ttttgattcc
     4141 atgttgctct ggaaaataat aacagagagt aatatacgta cagatatcag gttaaccgct
     4201 atcccctctc ttttgtcagg tatggtttcg tctctcattg tgcctgtttt tattatctgg
     4261 caactgaata aaatggatgt tgctctttat ggtgatgcga agtttgccag tgacagtgat
     4321 ttaaaaaaat caaaactgct taagtgggag acagaaaatg atacggatat tctggtcggc
     4381 gcgtataaag gaaaatatct gtggtatact gccccggatt ttgtatctct tggtgcagga
     4441 acccgcgccg gtaaaggagc cgccattggt atccctaatc ttctggtcag aaaacactct
     4501 cttattgcgt tagatccaaa acaggaattg tggaaaatca ccagtaaggt gcgtgaaata
     4561 ctgctgggga ataaagttta tcttctcgac ccattcaaca gtaagacaca tcaatttaac
     4621 ccccttttct atattgattt aaaagcggag agtggtgcta aggatctgct taaactgatt
     4681 gaaattctgt tcccgtctta cggcatgaca ggggcagaag cgcactttaa taaccttgct
     4741 ggtcaatact ggacgggact ggctaagttg cttcatttct ttattaatta tgagccgtcc
     4801 tggcttaatg agttcgggct taaacccgtt ttctcaatcg gttctgtcgt cgacctgtac
     4861 agcaatattg accgggaact gatactcagt aagcgggaag aactggagga aacaaacggg
     4921 cttgatgaaa acgcgctgta tcatttgcgc gatgctctga ccaaaatcag ggaatatcac
     4981 gaaacggaag atgaacagcg ttcaagcatt gatggttctt tccgtaagaa aatgagcctg
     5041 ttttatctcc caaccgttcg taaatgtact gatggtaatg atttcgatct ccgtcagttg
     5101 cgacgggaag atatcactgt ttatgtcggt gttaatgcag aagatatatc tctggcttac
     5161 gattttctga acctgttttt taacttcgtt gttgaagtga cattgcgtga aaatcctgat
     5221 tttgatccaa ccctgaaaca tgactgcctg atgtttcttg atgagttccc ttcgattggt
     5281 tatatgccaa ttattaaaaa ggggtcaggg tatattgcag gttttaaact taaactgctg
     5341 acaatttatc agaatatcag tcagctaaat gaaatctatg gtattgaggg agccaaaacg
     5401 ctgatgagtg ctcatccctg tcgtattatc tatgctgtca gtgaagagga tgatgccgcg
     5461 aaaatatcag aaaaacttgg atatattacc actacatcaa agagcacaag caagagccgt
     5521 ggacggtcaa catcacaggg cgaatcagaa agtgaagccc gaagagcact ggtgcttcca
     5581 caggagctgg gaacgctgga ctttaaagaa gagtttatca tcctgaaggg ggagaaccct
     5641 gttaaagcag aaaaggcact ttattacctt gatccgtatt ttatggacag gttaatgaag
     5701 gtcagtccta aactggcatc attgacgatg gaactaaata agacgaaaaa aatatttggt
     5761 gtgaaagggc ttaaatatcc gtcaaaagaa aaaatgctct ccgtaggaga gctggagtct
     5821 gaggttttac tatgaaaaag atacttatga tcagtattct tttcctcaca gcctgttcct
     5881 ctccacctga accgccacag gttgaatggg aaaaacggcc tgaagttatg aatacacaaa
     5941 taatgaactg gaaaccaaca tccggtgtta ttaaatcaga taatataaat tcctcatgga
     6001 gtaaggtgtt acctggtttt aaaccagaaa atcgtcttta cgatgattct gtcttttatg
     6061 ctgttgccca ctctgaaaaa atagttgtaa ggacatcttc ttttgatagt tactggtctg
     6121 cgaaatactg gctaagaaaa aatggtgcaa caggtgttat tgaatatcag ccactaaaaa
     6181 gatggttgaa tagtgactat gttgaaattt atctgtcaag aataaatgtt cagaggttac
     6241 catgaaaaag atatgcaaaa gtctaatcgt aatttttact gtgtcctggc tgacagcacc
     6301 tgcgtatgca tctgaccctt gcgcctcggt actttgctta tatggaaaag cagtcgggca
     6361 aggtggggga agtgagtgca gaagtgctga aaaggacttt tttaatattc tgaaaaagaa
     6421 aaaaggaaac attcgctgga gtaaaacatt tgatgcccgc aaggcatttt tgaatcagtg
     6481 ctcaacggcg gatccggctg ctatctcaaa aattatgagt aagttcggta agattaaggg
     6541 gtagatgata tgaaaaaaaa agaaaagatt gattttctta atgcaataaa agcagggcac
     6601 tctggcttta tagctggtaa tcctgctcat gcattggatg ataagactat gaacgccatc
     6661 attcataact acgaggcaac gattgcgccc ggtaaagcag gtaactgcaa agaaactcct
     6721 ctcacaaagt attgttcgag gactatgcca gagcgtattt cagttgaaga aattaatgag
     6781 gttaaaattt atccagagtt attctgaacg gagataacgg taaacgccgc agattccaga
     6841 ttttaatctg ggatttgcgg ggttgaacaa ccgaagggcg ttagtacaca tttttggtaa
     6901 tgaattatcg ggagataaaa atgatatcca ttaagagcta ttttaactgg aataagttat
     6961 ttatgttttt agctgtggcc ttatactggt tttggcgtgt tctatccaga agcacaaagc
     7021 tgatgccagt
//
LOCUS       DAJOTB010000211         7028 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJOTB010000211 DAJOTB010000000
VERSION     DAJOTB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.211"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            324..824
                     /gene="tssB"
                     /locus_tag="OHN28_004486"
     CDS             324..824
                     /gene="tssB"
                     /locus_tag="OHN28_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCQ2299758.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            883..2421
                     /gene="tssC"
                     /locus_tag="OHN28_004487"
     CDS             883..2421
                     /gene="tssC"
                     /locus_tag="OHN28_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HCQ2299759.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2439..3776
                     /gene="tssK"
                     /locus_tag="OHN28_004488"
     CDS             2439..3776
                     /gene="tssK"
                     /locus_tag="OHN28_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HCQ2299760.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3773..4438
                     /gene="tssL"
                     /locus_tag="OHN28_004489"
     CDS             3773..4438
                     /gene="tssL"
                     /locus_tag="OHN28_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HCQ2299761.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            4451..6103
                     /locus_tag="OHN28_004490"
     CDS             4451..6103
                     /locus_tag="OHN28_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCQ2299762.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6161..6652
                     /locus_tag="OHN28_004491"
     CDS             6161..6652
                     /locus_tag="OHN28_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCQ2299763.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6843..>7028
                     /locus_tag="OHN28_004492"
     CDS             6843..>7028
                     /locus_tag="OHN28_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299764.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEW"
BASE COUNT         1605 a         1903 c         1949 g         1571 t
ORIGIN      
        1 agcccggaca ataagtttca tgaatatctt aataggctct catactaccc aatttcttaa
       61 aagtgcaaat ttgtgaagct tatcctgtta ttattttaag tttcatgcca aattctctca
      121 ccagataatg ccgccctctc ccgaaaaata atcaagaggc caaacaatat ctaaaatgat
      181 acaactgtat ctattcccct gaaaaataca ttattcattt gtatattttc ctcatcattg
      241 ctttttattt aaatcatccg ataatcccct gaatataatt atgtcaataa ccatcagaaa
      301 aagtggatga tgaggaaaag gatatggccg acagtttcca gaatgaagtt cccgccgctc
      361 gtgtaaatat caagcttgac ctgcatacag gcaatgctaa aaagaaagtt gaactccccc
      421 tcaagcttct tgccgtaggc gattacagta acggaaaaga gcagcgtccg ctgtccgaac
      481 gggacaagat tgatatcaat aaaaacaact tcaacagcgt catggctgag ttttcgcctg
      541 cggttaattt aacagtagaa gatacgctaa acggaaacgg taatgaacaa aatatcgcgc
      601 ttgaatttaa aagcctgaag gattttgagc ccgaacaggt agcaaaaaat attccacaac
      661 ttcgtgtatt actcgctatg cgcaatttac tacgtgacct gaaatccaat ctgctggata
      721 acgcaacgtt tcgccgtgaa ctggaaaata tcctgaaaga cccgacgctc agttcagaac
      781 tgcgtgatga actggcgaaa attgcgccgc aggagaacgt ttaacccgaa cgcgtattca
      841 ggttaaaccg gagaagaaag gatcactgaa aaggaaatgc tgatgtctgt acaacaagaa
      901 catgccacct ctgaaactgc aacactcacc accactgagt ccggcggcgt ttatcagtcc
      961 ctgtttgata aaattaattt aaccccggtc tcttccattc aggaaatcga ttcatggcaa
     1021 aacagcgaaa cgctggccga tgcctcaccc gatgagcgcg tgacggcggc tatccacgtt
     1081 ctgctttcct gtctggcgaa gtctggcgag gacgtggtta aactcgacaa aagcctgctg
     1141 gattttcata tcgacgatct ggatcagaaa atcagtaaac agcttgatgc agtcatgcac
     1201 catccggaat tccagaaagt cgagtcgctc tggcgcggca catggttcgt cgtacagcgc
     1261 actgattttc gcaaaaatgt cagaattgaa ctgctggatg tcagtaaaga acatctgcgg
     1321 caggactttg atgattctcc ggaaatcatt caaagtggtt tatatcgcca tacatacatt
     1381 caggagtacg atacgccggg tggcgaacct gtagcctcat taatatccag ctatgaattc
     1441 gataacagcc cgcaggatat tgccctgctg cgtaatattt ccagagtctc tgctgcatca
     1501 catatgccct ttatcggttc cgtcggaccg aagttcttcc ttaaaaactc gatggaagaa
     1561 gtcgccgcaa ttaaagatat cggcaactat tttgaccgcg cggaatatat caaatggaag
     1621 tcgttccgcg ataccgacga cagtcgctac gtgggattag tgatgccgcg cgtgctgggc
     1681 cgtctgccct atgggccgga cacagtgccg gtacgcagct ttaactatgt ggaagaagtc
     1741 aaaggcccgg atcacgaaaa atacctctgg acaaacgcct cgttcgcctt tgccgccaat
     1801 atggtgaaga gctttgtgaa taatggctgg tgcgtgcaga tccgtggtcc gcaggcgggt
     1861 ggcgcagtgg ccgatctgcc gatccatctt tacgatctcg gcaccggcaa tcaggtcaaa
     1921 attccgtccg aagtgatgat cccggaaacc cgcgaatttg aatttgccaa ccttggcttt
     1981 attccgctct cttattataa gaatcgcgat tacgcctgct tcttctcagc gaactctgcc
     2041 cagaaaccgg cgttgtatga taccgctgac gccaccgcca acagtcgtat taacgcccgt
     2101 ttgccctata ttttcctgct gtcccgcatt gcgcattacc tgaaaatcat ccagcgcgag
     2161 aatatcggta ccaccaaaga ccgccgcgta ctggaactgg agcttaacac ctggatccgc
     2221 acactggtga cggagatgac cgatccgggt gatgaactgc aggcgtctca cccgctgcgc
     2281 gacggcaagg ttatcgtgga agatattgag gacaatccgg gcttcttccg cgtcagactc
     2341 tttgccgtgc cgcatttcca gattgaaggg atggatatca acctgtcact ggtttcccag
     2401 atgccgaaag cgaaagcctg accgggccgt ggtgtgatat gaaaattcat cgaccactgt
     2461 gggctgaggg gacattcctg tcctctcagc agtttcagca gcaggcccgc tgggaagcgt
     2521 tcagcaatga cagcattgcg caactttgta ttcgtcatcc gtgggggatc gcgaatgtgc
     2581 tctttgaccg cgatgccctg acgtccggaa agctgaaaac gcaggctgta cgtctgcgtt
     2641 ttgctgatgg cacgctgatc gattcagacg tgagtgacgt tctgccgctc gcctgcgatc
     2701 tgcgcgcgct gaagaacgac agcgcgattg tcctgctggc gctgccgctt gcgcatggta
     2761 acggcggtaa tctcggtcag ggtgagcaga ctgaaagacc gctgcgctat cgtcaggaat
     2821 ggcaaaaggt gcaggatatc tacggcagtg acagtgaaga tatggccgtt gagcgtcatg
     2881 cgctgtccct gcgctttgct catgataaca atcaggatta catcacctgc ccactggcgc
     2941 gtctggtgcg ggatgttcag ggcaactgga cacaggatga gagctatatt ccgccgttac
     3001 tggcgtttaa cgcccatgac gggctggtac agcgtctgga cacgctgctg ttgcagctac
     3061 gggcgaagtg ccagcgtctg atggcgatgc gccgggaaag caaccagcgt atggcggact
     3121 tcgctgtcgc cgatgtctcc ctgttctggc tgcttaacgc gctgaactcc gccgagccgg
     3181 tactgagtga tttcctgcgc tacccggcgg ttcatcccga actggtctgg cgggagctgg
     3241 cgaggcttgc cggtgcgctg ctgacgtttt cccttgaaca taacgtcagc gccgttccgc
     3301 cctatgtcca cgaatcgccg tccatcgtct ttccgccact tttctccctg ctcagcgaac
     3361 tgctggaagc cagcctgccg tcacgggtga tcgcgctgga tctggcgtcc ctgccgggca
     3421 accgctggaa agcagacctg cacgatccgc gcctgcggga agaggccgac ttttacctgt
     3481 ccgttcgttc ctctctgcct gcgcatcagg tactgcacca gctaccgctg gtctgcaaaa
     3541 tcggtgcgcc tgatgacgtc acgctgctta tcaatgtggc gctgaatggt gttccactgg
     3601 tgccgcttac ctcagttccg gcagcgctgc cactgcggct ggagaaccag tattttgcgc
     3661 tggatatgca cagtgacgcg gctaaatcga tgctggagtc cgggtgctgc atgatttatg
     3721 cgccgggaac aatgggcgac ctgaaacctg aactgtttgc ggtgctgcgc acatgaaaaa
     3781 agagacggat atcaatatcg acgcgctgct gcgcgacacg tttctgacgg tggtggaact
     3841 gcgtcaggga accaccgtgc gtcacggtat ggagctgtat cgccactgcc agcggcaggt
     3901 ggaactggtc cgcgaacgcc tgaaagatgc cgggttcagc cgggagagcg tggagcacat
     3961 tacttacgcg cagtgcgccc tgctggacga aacggtcctg agccgtggcg gcatggacga
     4021 cggtcaggcc atctggatga aggatccact ccagtcgcat ttcttcaaca ctctgcaggc
     4081 cggtgagctg ctgtacgagc ggatgaagca ggtgctacag gagcccgccc ccgcgcaggc
     4141 cgtgctgacc tgctttcacc gtgtcctgct gctgggattt cgcgggcgtt atcaggatcc
     4201 ggcagccccg gagcgcgacc agcttatttc gacgctcaac gggcaggtcg cgccatttgg
     4261 tgtgctgccg gaaacggcgg tactgaacgt gccgttatcc ccccgtcagc accctttgct
     4321 gcattcgccg tttttctggc tggtgacgct cacactggta ctggccggtg tctggtgggg
     4381 gctacattac tggctgaacg tgctggtgga tgaactgctg ccgcaaagtc tgcgttaaag
     4441 ggagtcgtga gtgaggaatg cgctgaaaca gacggttttg ttatggactc tgttgttgct
     4501 gctgactacc tggctgggtt tctttgctgt atctgcggtc gtgaagtatg ccgtggtagt
     4561 gattcttgtc gccgtcacag cggcggtgat ggcgtactgg cataagaaaa agggacaggt
     4621 cgtagcggac gacgtatggc cgtcatcgct accgccggaa acctaccgac agccggtagt
     4681 gctggtctgt ggcgatatgt cggcgcacct gttcactgac agcccggtcc ggcaggtttc
     4741 agaagggctg tatctgcctg tttctgatga agaacagctt gtggcgcagg tggagcgatt
     4801 actgaccctt cgcccggcgt gggcatcgca gtttgccgtg gcgtataccg tcatgcccgg
     4861 catgtaccgg gatgcggcgg ttctgaccgg acaactgcgc cggttcgctc acagtatggc
     4921 gacggtgcgt cgccgggcgg gcgtaaacgt accctggctt ctctggagcg ggctgtccgg
     4981 ctcgccgttg ccggagagag cgaattcacc gtggtttatc tgtaccggcg gcgaagttca
     5041 ggtagcaaca tccgcagaga ccaccatgcc cgcgcagtgg attgcacaat ccggcgcaca
     5101 ggaacgcagt cagcgactct gttacctgct gaaagccgaa agcctgatgc agtggctgga
     5161 tctgaatgtg ctggcggaac tgaacggccc ggaggcgaaa tgtccaccac tggcgatgac
     5221 cgttgggctg gtcccctcgt tgcctgcggt ggataacaac ctgtggcagt tgtggatcac
     5281 cgccagaacc ggcctgacgc cggatatcgc ggacaccggc acagacgatg cgctgccatt
     5341 cccggatgcc ctgttacggc ggttgccgcg tcagtcgggc tttaccccgc tgcgacgggc
     5401 ctgcgtgacc atgctgggcg tcaccaccgt ggcgggtatc gccgcgctgt gcctgtcagc
     5461 cacggcaaat cgccagttat tacggcaggt cggtgacgat ctgcaccggt tttatgccgt
     5521 cccggcggaa gaatttatca ccaaagcccg tcacctgtcg gtgctgaaag acgatgcggt
     5581 catgctcgat gggtattacc gggaaggaga accactacgt ctcggtctgg ggttataccc
     5641 cggtgaacgc atccgccagc cggtattacg cgccattcgc gactggcgtc cgcctgagca
     5701 aaaaatggac gtgacggctt cgcttccggt tcagaccgtg cgtcttgaca gtatgtcgct
     5761 gtttgacgtc ggacaggccc gcctgaaaga cggctcgaca aaagtgctgg tggatgcgct
     5821 ggtgaacatc cgggcaaaac cgggctggct gatcctcgtg gccggatata ccgatgccac
     5881 cggcgatgaa aaaagcaatc agcagttatc gctgcggcgt gccgaagcgg tgcgcaactg
     5941 gatgctgcag accagcgaca tcccggccac ctgttttgcc gtacagggac tgggcgagag
     6001 ccagcctgcg gcgaccaacg acacgccaca gggccgggca gtcaaccggc gtgtcgaaat
     6061 cagtcttgtt ccgcgttctg acgcctgtca ggacgtgaaa taaaacatac cgccggaaga
     6121 aggcggtgct tcaatcacac taacaaggag agtaattctc atggctattc ctgcttatct
     6181 ctggctgaaa gatgacggcg gcgcggatat caaaggttcc gtggacgttc aggggcgcga
     6241 aggtagcatc gaagtggtgg cgctggatca cgatgtgtac atcccgaccg acaacaacac
     6301 cggcaaactg accggtaccc gtactcacaa gccctttacg tttaccaaag aaatcgatgc
     6361 ctccagcccg tatctctaca aagctgtgac caccggccag actctgaaaa cggcagaatt
     6421 taagttttac cgcatcaacg atgccggtca ggaagtggag tacttcaaca tcacgcttga
     6481 taacgtcaag ctggtcagag tcgctccgct tatgcacgac atcaaggatc cttccagaga
     6541 gaagcataac cacctggaac gtattgagtt ccgctacgag aaaatcacct ggacttacaa
     6601 agacggcaac atcattcatt ccgactcgtg gaatgagcgt ccttccgcct gataactgcg
     6661 gcagacagga aactgtctgc ctttttgttg tgtgctttgt ccagtgccgc aggcaccggt
     6721 actgagcaaa gcatacccac aatctggtga aacctgatgg gcctgagtga ggtaacggct
     6781 atgggcggta gcgtgcctga acgcggatcg caggaataca gaacacgata aggaaacctt
     6841 ctatgaccgg aaatcacccc gccgcgctgc tgcgtcgcct taacccatac tgtgcacggg
     6901 cgctggacgc tgccgcctca ctgtgtcaga cccgcgccca tgcggaaata accattgaac
     6961 actggctgct gaaactgctg gagcagggag aaggcgatat cacggtgatt gcccgccgct
     7021 atgaatgg
//
LOCUS       DAJOTB010000212         6816 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJOTB010000212 DAJOTB010000000
VERSION     DAJOTB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.212"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1133
                     /gene="emrK"
                     /locus_tag="OHN28_004493"
     CDS             <1..1133
                     /gene="emrK"
                     /locus_tag="OHN28_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCQ2299765.1"
                     /translation="DRRKYFALLAVVLFIALSGAYAYWSMELKDMISTDDAYVTGNAD
                     PISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYL
                     QDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAI
                     QAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGET
                     VSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINM
                     GTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDI
                     AEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1133..2671
                     /gene="emrY"
                     /locus_tag="OHN28_004494"
     CDS             1133..2671
                     /gene="emrY"
                     /locus_tag="OHN28_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCQ2299766.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     LQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2779..4107)
                     /gene="dsdA"
                     /locus_tag="OHN28_004495"
     CDS             complement(2779..4107)
                     /gene="dsdA"
                     /locus_tag="OHN28_004495"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCQ2299767.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4125..5462)
                     /gene="dsdX"
                     /locus_tag="OHN28_004496"
     CDS             complement(4125..5462)
                     /gene="dsdX"
                     /locus_tag="OHN28_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HCQ2299768.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            5680..6615
                     /gene="dsdC"
                     /locus_tag="OHN28_004497"
     CDS             5680..6615
                     /gene="dsdC"
                     /locus_tag="OHN28_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HCQ2299769.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVSLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1890 a         1546 c         1567 g         1813 t
ORIGIN      
        1 ctgacagaag aaaatatttt gctttgttag cggtagtttt atttattgcg ctttcaggtg
       61 cctatgccta ttggtcaatg gaattaaaag acatgattag tacagatgac gcctatgtca
      121 cgggaaatgc agatccaatt tctgcacaag tctcaggcag tgtcactgtc gttaatcata
      181 aagatacgaa ctacgttcga caaggtgaca ttttagtttc actggataaa actgatgcca
      241 ctatcgcact caataaagct aaaaataatc tggcaaatat tgttcggcaa acgaataaac
      301 tatacttaca ggataaacaa tacagtgccg aagtcgcttc agcacgtatt cagtatcaac
      361 aatctttaga agattataac cgtcgtgtgc ctttagcgaa gcaaggggtt atttcaaaag
      421 aagcgctgga gcataccaaa gatacgttaa taagtagcaa agcggcattg aatgctgcta
      481 tccaggctta taaagcgaat aaagctttag taatgaacac accattaaac cgtcagccac
      541 aagtcattga agctgcagat gcaaccaaag aagcctggtt ggcgcttaaa cgtacggata
      601 ttaagagtcc ggttaccggt tatattgccc agagaagtgt tcaggtcggc gaaacagtga
      661 gtcccggaca atcgttaatg gctgtcgtac cggcgcgtca aatgtgggtt aatgccaact
      721 ttaaagaaac acaactcacg gatgtacgga ttggtcaatc ggtcaatatt atcagcgatc
      781 tttatggtga aaatgttgtg tttcatggtc gggtgacagg gatcaatatg ggaaccggca
      841 atgcgttctc cttattacct gcacaaaatg cgacagggaa ctggatcaaa atcgttcagc
      901 gtgtaccggt tgaagtttct ctggatccaa aagaactcat ggaacaccct ttgcgcattg
      961 gtttatcgat gactgcaact attgatacga agaatgaaga cattgccgag atgcccgatc
     1021 tggcttcaac cgtgacgtcc atgcctgctt ataccagtaa ggctttagtt atcgatacca
     1081 gtccgataga aaaagaaatt agcaacatta tttcgcataa tggacaactt taatggcaat
     1141 cactaaatca actcctgcac cattaaccgg tgggacgtta tggtgcgtca ctattgcatt
     1201 gtcattagcg acgtttatgc aaatgttgga ttccactatt tctaacgtcg caataccgac
     1261 aatatctggc tttctgggag catcaacaga cgaaggtacc tgggttatca cctcgtttgg
     1321 cgtagcaaat gccattgcga tccctgttac cggcaggctg gcacaaagaa taggcgaatt
     1381 aaggttattt ttactttcag taaccttttt ttcgctgtct tcattaatgt gtagtttatc
     1441 gaccaatctt gatgtgctga tattttttag agtcgttcag gggttaatgg cggggccgtt
     1501 aattccactg tcacagagtt tattattaag gaattatcca ccagaaaaaa gaacatttgc
     1561 tctggcatta tggtcaatga ccgtgattat cgctccgata tgtgggccga tattgggcgg
     1621 ctatatttgc gataacttta gctggggttg gatattttta atcaatgtcc ctatggggat
     1681 tatcgtcctg acattatgct taaccttact taaaggacga gaaactgaga cttcaccggt
     1741 caaaatgaat ctaccaggac tgaccctgtt agtgctcggt gttggtggct tgcaaattat
     1801 gcttgataaa gggcgcgatc tggattggtt caactcgagt acaataatac tactaacagt
     1861 agtatcagtt atttctctga tctctttagt catttgggag tcgacctcag agaatccgat
     1921 tcttgacctc agtttgttta agtcccgtaa tttcaccatt ggtattgtga gtatcacatg
     1981 cgcgtattta ttttactctg gagcgatagt tcttatgccg cagttactcc aggaaacgat
     2041 ggggtataat gcgatatggg ccggacttgc ctatgcgccc atcggtatca tgccgctatt
     2101 aatatcacct ttgataggac gttatggcaa caaaattgat atgcggttgt tggtgacatt
     2161 tagttttttg atgtatgcgg tttgctatta ctggcgttct gtgacattta tgccaacgat
     2221 tgattttaca ggcatcattt tgccacagtt ttttcaggga ttcgccgttg cctgtttctt
     2281 tttaccctta acaacgattt cgttttcagg cttgccagat aataaatttg ccaatgcctc
     2341 gagtatgagt aatttttttc gcaccttgtc aggatcagtt ggtacgtcgt tgacaatgac
     2401 actgtgggga cgccgcgaat cgttacacca tagtcagttg acagcaacca tcgatcaatt
     2461 taaccccgtg tttaattcat cgttacaaat tatggataaa tactatggtt cgctttcagg
     2521 agttcttaat gaaattaata atgaaataac tcagcagtca ctttctattt ctgcaaatga
     2581 gattttccgt atggcggcta ttgcttttat cttacttacg gttttggttt ggtttgcgaa
     2641 accgccgttt acagcgaaag gcgttgggtg atggaaaaag aggaggctat agcaggtaaa
     2701 acggcagaat cttaagtctg cgtcatcggc agcatcgctc acccagggaa aggatggtga
     2761 tgctgcgttg aaacgttatt aacggccttt tgccagatat tgattcatct cttcttccgg
     2821 caccattcca cctcccgtcg cccacaccag atgagtggca ttacgcagtt gttctgcgct
     2881 gaaaccgtgc atctgttggt aacttactga tgcgcacacg cgctgaggtc cggccatacc
     2941 cgccagtgcc gaaggttcaa gacgaatacc ttcttcctgc gccagccagc caagcatgtc
     3001 atacatggtt tgatcgctaa gggtatagaa gccatccagc agacgctcca tcgcccgccc
     3061 gacaaagccc gatgcgcgac caaccgcaag gccatcggca gcggtaaggt tgtcgatgcc
     3121 aatatcctga acagaaatct gatcgtgtaa tccggtatgg acgcctaata acatacacgg
     3181 ggagtgtgtt ggttcggcaa aaaagcagtg aacatgatcg ccaaacgcca gtttaagtcc
     3241 gaatgcgacg ccaccaggac caccgccaac accacacggc agatagacaa atagagggtt
     3301 atcagcatcg acgatacggc cttgctgggc aaattgcgct ttaagacgct ggccagcgac
     3361 ggaataccca aggaacaacg tgcgggaatt ctcgtcatca ataaagaaac agttcgggtc
     3421 agactgcgat gctttacgtc cttcctcgac agcaacacca taatcttgct catattccac
     3481 gaccgtaacg ccatggctgc gcagtttcgc ttttttccat gcccgggcat cagcagacat
     3541 atgcactgtc accttaaagc caatgcgggc gctcataata ccgattgata accccagatt
     3601 tccggttgag cccacagcaa tgctgtattg gctaaagaac tgtttaaact ccggagaaag
     3661 cagtttgctg tagtcatctt caagcgtcag caaccctgct tccagagcca gtttttctgc
     3721 atgtgccagg acttcataaa tcccgccgcg tgcttttatg gagccggaaa taggcaaatg
     3781 gctatctttt ttcagtaaca gttgcccgct gatcggttgc tgatattcct tttccagccg
     3841 tttttgcata gccggaatgg caaccagttc tgattcaata atccccccag tggcagcagt
     3901 ttcaggaaat gcttttgcca gatagggggc aaaacgagat aagcgcgcat gggcgtcctg
     3961 aacatcctgt tcggtcaggc caacataagg taaaccttca gccaatgagg tcgtgccagg
     4021 attgaaccaa gtggtttctt taagagcaac cagatccttt accaacggat actgggcgat
     4081 gagcgaattc attttagcgt tttccatagt acgtctcttt gcgcttagat gataaatgac
     4141 agcaggaatg tgcccgccag agcgatgacc gaagcgataa atgtcgctgt cgtatagtat
     4201 ttaaatgttt cattgagcgt agcgccgcaa tattgcttca ccagccagaa aagcgagtcc
     4261 gtaacgatcg tgcagccaat cgcaccggaa ccgatagcaa tcgcaataat ttccgggctg
     4321 atgtcgggat acagcggcag catgggggca acaattgccg ttgcccccat cattgccacc
     4381 gtagcggagc ccactgcagc atgcagaata agtgccacca accaggccag aagaatcggg
     4441 tgcatatgca tattggagag aataactgcc agcgtatcag cgagactgct gctttttaaa
     4501 atggcgttga atgcgcctcc ggccccgata atcagcaaaa tattagcaat agaaccgaaa
     4561 ccattttccg tatgtgtgag catcgtcccc atgctcatat gctgacgtat acccaacaca
     4621 taataggcga caaacacggc gataaacatg gcagtgatag ggttgccaat aaactcaagc
     4681 aaggtataca aaccactctc acgcgccata ttcaattcgg caatcgtttt aaccaacatc
     4741 agcgcaatgg gtagcagtat ggtgaataac gttgcgccta atgaaggtag tgttttttca
     4801 tcgcgaactt tgagatcggc aaactctgtg ggtacaggtt taaagggcag tcgttgcccc
     4861 agaaatttaa ggaaaagtgg gccaccgatc agtgatgcca tcagcccaac cagcaaaccg
     4921 tagacgatca ccgaaccgat atctgcgccc agcttattgg caacatataa agcagccgga
     4981 tgtgggggaa ccacgcagtg cacagccatc aatgcggtac acagcggaat ggccagcttt
     5041 aacaatgagg tattggtttt tttggcaatt gaaaaagcca gaggaatcaa tagcacgacg
     5101 cccacttcaa caaacagcgt gatgccgcaa atcaggccaa ccagcaccat aatgacatca
     5161 gccgaaagcc accggcagcg ttgaagtgtc agaccaattc tttctgcggc cccggatact
     5221 tccatcattt ttcccagtat cgtgccaagg ccgataaccg cagcgaggaa ccccagcgtt
     5281 ccgccaattc cactttcaat agcatttacc atatccagtg gccccatacc catcatcgtt
     5341 cccacgaaga agctggccag caacagcgcc agaaacgggt ggaatttgaa cttcacgatg
     5401 gtcagtacaa ttaacacgat gctgataagc agcgtgctca caacccagat ttgagagtgc
     5461 atatctcacc ttcccctgtg attaacctga tgatattgga caaaaaaaca acgcaggctg
     5521 acaaacgata aaatttgcct ttcacatgag ctaaattgca tcaagggagt gatttacatc
     5581 taaaaatgaa ggtgaattga gatatggttc actttagctc accttacagc tgaaattttg
     5641 ccgctatttt tttacactta atagaaaaat gaggtgatta tggaacccct tcgtgaaata
     5701 agaaatcgac tgcttaacgg ctggcaacta tcaaaactgc atacttttga agtggctgcc
     5761 aggcatcagt ctttcgccct ggcggcagag gaattgtcgc tgagccccag tgcggtaagt
     5821 caccgtatca atcagctgga agaagaatta ggtattcagt tgtttgttcg ttcccatcgc
     5881 aaagtggaat taacgcacga ggggaaacgc gtttattggg cgctaaaatc gtcgctggat
     5941 accctgaatc aggaaattct ggatatcaaa aatcaggagt tatcgggaac gttaactctg
     6001 tattcccggc cctctatcgc ccaatgctgg ttggtgcccg cattaggtga ctttacacgc
     6061 cgatatccgt ctatttcgct caccgtgctc actggtaatg acaatgtcaa tttgcaacgt
     6121 gccggaatcg atttggcgat ctactttgat gatgcgccgt cagcgcaact gacccataac
     6181 tttctgatgg atgaagaaat cttgccagtt tgtagcccgg agtacgctca tagacatgat
     6241 ttaaccaaca cggtaattaa cctgcgtcac tgtacgttgc tccatgacag acaggcatgg
     6301 agcaacgact ccggtacgga tgaatggcat agttgggcgc aacattatgc ggttagtttg
     6361 ccgacatctt ctggaattgg ctttgatcgt tctgatttag ccgttatcgc cgcgatgaat
     6421 catgttgggg tggcgatggg aaggaaacgc ctggtacaaa aaaggcttgc cagtggtgag
     6481 ctcgtcgcgc cgtttggcga tatgacggtg aaatgccatc agcattatta catcaccaca
     6541 ttaccgggca ggcagtggcc aaaaattgag gcatttatta cttggttaag agaacaggta
     6601 aaaaccactt cataatgaat aagaccacga gttcctggtg tgccttattc ggcactgcta
     6661 gtgcgaattt aataatttac ggtttttacg tggttttagg gataaacaca agaatggtgt
     6721 cccctgcagg aatgtgatta atgaattaag aaattgattt ttatgaatat aaaattttta
     6781 ttgtcataat tctatacact tagttataca cataag
//
LOCUS       DAJOTB010000213         6642 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJOTB010000213 DAJOTB010000000
VERSION     DAJOTB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.213"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2228
                     /gene="nrfB"
                     /locus_tag="OHN28_004498"
     CDS             <1..2228
                     /gene="nrfB"
                     /locus_tag="OHN28_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCQ2299770.1"
                     /translation="LDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRRIKRK
                     LSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHIFVGT
                     YPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANFAFAG
                     FILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSELHGKD
                     VPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKEKGMT
                     EIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGIVFQG
                     FKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWHFLSI
                     FSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFMANWR
                     ALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTALRNR
                     VEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVALHYA
                     VLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYARRRG
                     HDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLLLRHE
                     RSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLESENEG
                     E"
     gene            2215..5187
                     /gene="nfrA"
                     /locus_tag="OHN28_004499"
     CDS             2215..5187
                     /gene="nfrA"
                     /locus_tag="OHN28_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCQ2299771.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPASLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WSFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5188..6078
                     /locus_tag="OHN28_004500"
     CDS             5188..6078
                     /locus_tag="OHN28_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCQ2299772.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISIFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         1638 a         1720 c         1723 g         1561 t
ORIGIN      
        1 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
       61 tcattatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
      121 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
      181 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
      241 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
      301 ttggcaccta ccccaacgac cccgatactc agcgtgatgt tgacgaagtg tgcgctcgct
      361 tcccgaacgt gcataaggta gtctgcgcgc gtcctggccc caccagcaaa gccgactgtc
      421 tgaacaacgt gctggacgcc atcacccaat ttgaacgtag cgccaatttc gcttttgctg
      481 gttttattct gcatgacgcc gaagatgtga tttcaccaat ggaattgcgt ctgttcaact
      541 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgttcgaa cgcgaatgga
      601 cgcacttcac cagcatgacc tacattgatg agttttcaga actgcatggc aaagatgttc
      661 cggtgcgtga agccctcgcc ggacaggtgc ccagcgcagg agtcggcacc tgtttcagcc
      721 gccgcgccgt gaccgcactg ttagctgacg gtgacggtat tgctttcgac gtgcagagtc
      781 tgactgaaga ttacgacatt ggcttccgcc tgaaagagaa aggtatgacg gaaatttttg
      841 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcatg
      901 cgcggacgtc aaacatgatc tgtgtgcgcg aatatttccc cgataccttt tcgactgcgg
      961 ttcggcaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccacaaat
     1021 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     1081 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     1141 aaagtttgtg gcccgatgcc tggcatttcc tttctatttt cagcggcagc gcatggttaa
     1201 tgactctgct gtggctaaac tttggcttga tgattaaccg catcgtgcag cgggtgattt
     1261 tcgttactgg ctactacggc ctgacgcagg ggctgctatc tgtcctgcgt cttttctggg
     1321 gcaacctgat taacttcatg gccaactggc gcgcgttaaa acaggtactt caacacggcg
     1381 atccacgtcg tgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtg actggcgata
     1441 cccgctcgtt gcgtccgtta ggtcaaatcc tgctggaaaa tcaggtcatc actgaagaac
     1501 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     1561 tgcaggggct gattagcgcc gagcaactgg cacaggcgct ggcagagcaa aacggcgtgg
     1621 cgtgggaatc catcgatgcc tggcagatcc cttcctcgct gattgccgaa atgccggcct
     1681 ccgtggcgtt gcattatgcg gtactgccgc tgcgtctgga caatgatgag ttaattgtcg
     1741 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     1801 aagtgcgtta cgtcattgtt ctgcgaggac aaattgtcac cggattacgc cactggtatg
     1861 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcagttcag catcagtggc
     1921 tcacggaaca gcaggccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     1981 cagaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     2041 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttattagcc
     2101 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     2161 tattactcaa agcaggttta aacactgaac aggttgcaca actggagtcc gaaaatgaag
     2221 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacatc tttattgagt
     2281 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     2341 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     2401 gaagcaaccg ccatccgcga atttgaatat atacaccagc aggtgccgga taatattccg
     2461 ctgactttat accttgcgga agcctatcgc cattttggtc atgatgaccg ggcgcggctg
     2521 ttgcttgagg atcaactgaa acgtcaccct ggagatgacc ggcttgagcg cagtctggcg
     2581 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     2641 gcgtgcgatg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa tgccctgcgg
     2701 ctggcacaat tacctgtcgc cagagcgcaa cttaacgatg cgacgtttgc tgcatcgccg
     2761 gaaggaaaaa cgttgcgaac cgatctgctg caacgggcaa tctacctgaa acaatggtcc
     2821 caggcagata cgctatataa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     2881 cgtcagtggt ttgacgtgct tcttgccgcg cagctggatg atcggatcct ggcgctgcaa
     2941 tcacagggga tcttcaccga tcctcagtca tatattactt atgcgaccgc gctggcttat
     3001 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     3061 gacgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     3121 gcgttggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     3181 ctaccagtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     3241 cccgccaatg aaatgcttga ggagcgttat gccgttagcg tagcaacccg taacaaggct
     3301 gaagctctgc gtctggcacg attgctgtat cagcaagaac cggcaagtct tacccgtctg
     3361 gatcaactga cctggcaact gatgcagaac gggcagtcac gcgaagctgc cgatttattg
     3421 ctgcaacgct atccttttca gggcgatgcg cgtgtcagcc agacgttaat ggcgcgactg
     3481 gcgtctctgc tggaaagtca tccttacctg gcaacgccgg cgaaggtggc aattttatcg
     3541 aaacctttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgctgat
     3601 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcttacga tgctgccgcc
     3661 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     3721 cttcaggccg aacaacgaca accggacgcc tggcaacatc gtgcggtagc ctatcaggcg
     3781 tatcaggttg aggactatgc caccgcactg acagcctggc agaaaatcag tcttcacgac
     3841 atgagcaatg aggatctgct tgctgccgcc aataccgccc aggcggcagg aaatggcgcg
     3901 gctcgcgatc gctggttaca acaggcagaa caacgtggac tgggaaacaa tgccctctac
     3961 tggtggctgc atgcgcaacg ttacattcca ggtcagccgg aactcgcact gaacgatctc
     4021 acgcgctcaa tcaatattgc gccttctgcc aacgcttacg ttgcgcgggc aacaatttat
     4081 cgccaacgtc ataatatccc ggcggcggtg agtgatttgc gcgccgcgct ggaactggaa
     4141 ccgaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
     4201 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc taccggacga tccggcactt
     4261 atccgacaac tggcctacgt gaaccaacgt ctggatgaca tgcctgcgac gcagcactac
     4321 gcccgactgg tgattgatga cattgataat caggcgctga taaccccact gaccccagag
     4381 caaaatcagc aacgcttcaa tttccgccgt ctgcatgagg aggtcggtcg ccgctggtcg
     4441 ttcagtttcg attcttctat cggcttgcgt tccggcgcaa tgagtaccgc taacaataat
     4501 gtcggcggcg cagcgccagg gaaaagctat cgtagctacg ggcaactgga agccgagtac
     4561 cgcctcggac gcaatatgct gctggaaggc gacctgctct cagtttatag ccgcgtcttt
     4621 gccgataccg gtgaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc cggcaccggt
     4681 ctgcgctgga agccactgcg cgatcagatc tttttcctcg ccgtcgaaca gcagttgccg
     4741 ctgaacggac aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc attctttaat
     4801 ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc ccaaaacctg
     4861 tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc agattatcgc
     4921 gtcagctggc atcagaaggt ggcgaacggg cagactattg agccttatgc tcacgttcag
     4981 gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggtgt cggggtccgc
     5041 tggaatatct ggaccggcga gacgcactac gacgcctggc cgcacaaagt cagtctcggc
     5101 gtcgaatatc aacatacctt taaggcgatt aatcaacgta acggagagcg caacaacgcg
     5161 tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt gctgacactg
     5221 cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc acaaaaccgt
     5281 gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg tttgcaaggc
     5341 ttcgatacac tggttttgca atggacccgt tacggcgatg cattcaccca gccagaacag
     5401 cgcgcgttat tgtttaagcg tgctacagct gcgcaacagg ctggcctgaa gctgattgtc
     5461 gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtccgc agcgctggaa
     5521 agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg gagcgccgtg
     5581 cctggcgtaa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct gaactggcgc
     5641 agcgaagccg cccgtcagcc tttgctaaca tggttaaaca acgcgcagcg gttgattagc
     5701 gatgtttcag caaaaccggt ttatatcagt atttttttcg ccggaaacat gtcgccagat
     5761 ggctatcgcc aactgctgga acaagttaaa gcaaccggtg ttaatgtctg ggtacaggat
     5821 ggcagcggcg tggataaact gaccgctgaa cagcgtgaac gttatctaca ggccagcgcc
     5881 gattgccaaa gttccgcccc agcaagcggc attgtttatg aactgtttgt cgctggtaaa
     5941 ggtaaaacgt ttacagcgaa accgaaaccg gatgcagaga tcgcctcgct gttagcgaaa
     6001 cgttcctctt gcggtaaaga cacgctctat ttctctctgc gctatttgcc cgtcgcgcag
     6061 agtatccttg agtattaatg attaaaggga gcaacttagc ttgctccttt ccttttcact
     6121 ttcctaatca aaatcctggc aagatgaatt atttagcaga atatctgttt tcattttatt
     6181 cgtctgacgt gttccaatta tttagcaaaa ccgcgacaac aaagtgatgg tgtaatttgt
     6241 aaaagggaac aaataaatgg ctcgccctgc aggattcgaa cctgtagccc acggcttaga
     6301 aactccagga acgacatttt aagtcaacaa attaccgcgc catctctgcg ttcacacgtc
     6361 ccactacctc aaaatatgta aaaccttgca agccattgcg aggccttatg tgtctcagtt
     6421 ttgttcctct tttttatact aaaaaacaca gcaattgagg ataaacctca tgctattatc
     6481 gctaatatgc atctaaaggc atggcacttg aatagataaa aactccacaa agaaaaaaat
     6541 aattagccca taaaaccaca atgcaaaaca caaacagaca attaaactaa aaaactgaca
     6601 gtgcattgtg acaaaaattc aatgttaaac aaaatataaa ca
//
LOCUS       DAJOTB010000214         6484 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJOTB010000214 DAJOTB010000000
VERSION     DAJOTB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.214"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            184..456
                     /locus_tag="OHN28_004501"
                     /pseudo
     CDS             184..456
                     /locus_tag="OHN28_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            519..1105
                     /locus_tag="OHN28_004502"
                     /pseudo
     CDS             519..1105
                     /locus_tag="OHN28_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            1164..3674
                     /gene="papC"
                     /locus_tag="OHN28_004503"
     CDS             1164..3674
                     /gene="papC"
                     /locus_tag="OHN28_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HCQ2299773.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPALSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLAASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            complement(3706..3801)
                     /locus_tag="OHN28_004504"
                     /pseudo
     CDS             complement(3706..3801)
                     /locus_tag="OHN28_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3791..3994)
                     /locus_tag="OHN28_004505"
                     /pseudo
     CDS             complement(3791..3994)
                     /locus_tag="OHN28_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4079..4990)
                     /gene="arcC"
                     /locus_tag="OHN28_004506"
     CDS             complement(4079..4990)
                     /gene="arcC"
                     /locus_tag="OHN28_004506"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2299774.1"
                     /translation="MERKPTLVVALGGNALLKRGEPLEAEIQRQNIELAARTIAGLTV
                     NWRVVLVHGNGPQIGLLALQNSAYDKVTPYPLDVLGAESQGMIGYMLQQALKNSLPQR
                     EVSVLLTQVEVDATDPAFSNPTKYIGPVYNEDLAKTLAAEKGWGFKADGSYFRRVVPS
                     PQPKRIVESDAITALIQRDHLVICNGGGGVPVVEKANGYRGIEAVIDKDLSAALLAYQ
                     IGADALLILTDADAVYLDWGKPTQRPLAQVTPELLRGMQFDTGSMGPKVAACCKFVEA
                     CNGIAGIGALVDGAEILAGKKGTLIRN"
     gene            complement(5001..6221)
                     /gene="arcA"
                     /locus_tag="OHN28_004507"
     CDS             complement(5001..6221)
                     /gene="arcA"
                     /locus_tag="OHN28_004507"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCQ2299775.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRDQGVEVLLLTDLLTQTLDIKEAKTWLLETQISDYRLGPTFAGD
                     VRSWLADMPHRELARRLSGGLTYGEIPAAINNMVVDTHTSNDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPAFADGDFIKYFGDENIYYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLANSLFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDVDTFSVYPEVVRKDAQCWTLTSNGRDGLQRTQETDLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTIRPGVVIGYERNVWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLERDGI"
BASE COUNT         1507 a         1523 c         1780 g         1674 t
ORIGIN      
        1 gcggaggtgg ctcaacctgc gaaaagaaac gagttgctat ggacttgcac cggttgtgtt
       61 ccggtctatc tctcatttta agcggctttt ttctcaaatg ccaccggcga tttccagccg
      121 agtgctgatg aatcccctaa tgatttttac caaaatcatt aggggattca tcagggtcaa
      181 gcagctaaaa acggtaaggt tcaattatct tttactgggc cacaagtaac ggggcaggct
      241 gaagaattag caactaacgg cggtacgggc acagctattg tagttcaggc tgcaggcaaa
      301 aacgtttctt tcgatgggac tgcaggtgac gcttatcccc tgaaagatgg tgataatgtt
      361 cttcattata cagctcttgt gaaaaaagcg aatggcggta ctgtttctga aggtgctttt
      421 tctgcagttg caacctttaa tttaagttac cagtaattca tctgattttt acagggtgag
      481 gcaagaccca ccctgtgtct gacaacgaca tttaaggtat gaagaatttt tgtgttctgt
      541 ttgtgatgta tttctggagt acattacttc atgctgtatc ttcagagcca tttcctccgc
      601 ccggcatgac ccttcctgaa tactggggag aagaacacgt atggtgggac ggcagggctg
      661 cttttcatgg tgaggttgtc agacctgcct gtactctggc gatggaagac gcctggcaga
      721 ttatcgatat gggggaaacc ccggttcggg atttacagaa tggtttttcc ggacctgaaa
      781 gaaaattcag cctccggctc aggaactgtg aatttaacag tcagggtggg aaccttttct
      841 ctgattcccg gataagggtg actttcgatg gctccggggt gaaacgccgg ataagtttaa
      901 tttatccggt caggcaaaag gaattaatct gcagatagct gatgcctggg ggaatattgc
      961 ccgggcaggg aaagtaatgc ctgcaatacc attgacgggt aatgaagaag cgctggatta
     1021 caccctcaga attgtgagaa acggaaaaaa acttgaagcc ggaaattatt ttgccgtgct
     1081 gggattccgg gtcgattatg agtgagtcac tccggtgaga tgtccggtta tttatctttt
     1141 ttgtgaatct ggtgatgcgt ggaatgaaag acagaatacc ttttgcagtc aacaatatta
     1201 cctgtgtgat attgttgtct ctgttttgta acgcagccag tgccgttgag tttaatacag
     1261 atgtacttga cgcagcggac aagaaaaata ttgacttcac ccgtttttca gaagccggct
     1321 atgttctgcc ggggcaatat cttctggatg tgattgttaa cgggcaaagt atttctcccg
     1381 catcgttaca gatttcattt gttgaacctg cgttgtcagg agataaggca gaaaaaaaat
     1441 tgccgcaggc ctgtctgaca tcagatatgg tcagactgat ggggttaaca gcagaatctc
     1501 tggataaagt tgtttactgg catgatggtc agtgtgcgga ttttcatggg ttgccgggag
     1561 tggatattcg tcctgatacc ggagcgggcg tattacgcat caatatgccg caggcctggc
     1621 ttgagtattc tgatgccacc tggctgcctc cctcacgctg ggacgacggc attcccggac
     1681 tgatgctgga ttataacctc aacgggacgg tttcccgtaa ttatcaggga ggagactctc
     1741 atcagttcag ttataacggg actgtggggg ggaatctggg gccctggcgc ctgcgggctg
     1801 actatcaggg aagccaggag cagagccgct acaacgggga aaaaacgaca aacagaaatt
     1861 tcacatggag tcgcttttat ctgttccgtg ccattccacg atggcgggca aacctgacgc
     1921 tgggcgagaa taatatcaac tcagatatat tccggtcatg gagttatacg ggagccagcc
     1981 tggaaagcga tgaccggatg ctgccgccca gactgcgagg ctatgcaccg cagattaccg
     2041 ggattgcgga gactaatgcc cgtgttgtgg tgtcgcagca gggacgggtg ctgtacgact
     2101 cgatggtccc cgcagggcca ttcagtattc aggacctgga cagttcagtt cgcggacgtc
     2161 ttgatgttga ggttattgaa cagaatggac ggaagaaaac ctttcaggtc gatacagcct
     2221 cggttcctta tctgacgcgt ccgggacagg tccggtataa acttgtctcc ggtcgttccc
     2281 gtggatacgg gcatgagacc gaagggcctg tatttgcgac cggagaggca tcctgggggc
     2341 tcagtaacca gtggtcgctg tatggcgggg ctgtgcttgc cggtgattat aatgcactgg
     2401 cagccggtgc cggctgggac ctgggtgtgc cggggaccct ttccgctgat atcacgcagt
     2461 cagtagcccg tattgaggga gagagaacgt ttcagggaaa atcctggcgt ctgagctact
     2521 ccaaacggtt tgataatgcg gatgccgaca ttacgttcgc cgggtatcgt ttctcagagc
     2581 gaaactatat gaccatggag cagtacctga acgcccgcta ccgtaatgat tacagcagtc
     2641 gggaaaaaga gatgtatacc gttacgctga ataaaaacgt ggcggactgg aacacctctt
     2701 ttaacctgca gtacagccgt cagacatact gggacatacg gaaaacggac tattatacgg
     2761 tgagcgtcaa ccgctacttt aatgttttcg gactgcaggg tgtggcggtt ggattggcag
     2821 cctcaaggtc taaatatctg gggcgtgata acgattctgc ttacctgcgt atatccgtgc
     2881 cgctggggac ggggacagcg agctacagtg gcagtatgag taatgaccgt tatgtgaata
     2941 tggccggcta cactgacacg ttcaatgacg gtctggacag ctacagcctg aacgccggcc
     3001 ttaacagtgg cggtggactg acatcgcaac gtcagattaa tgcctattac agtcatcgta
     3061 gtccgctggc aaatttgtcc gcgaatattg catccctgca gaaaggatat acgtctttcg
     3121 gcgtcagtgc ttccggtggg gcaacaatta ccggaaaagg tgcggcgtta catgcagggg
     3181 gaatgtccgg tggaacacgt cttcttgttg acacggatgg tgtgggaggt gtaccggttg
     3241 atggcgggca ggtggtgaca aatcgctggg gaacgggcgt ggtgactgac atcagcagtt
     3301 attaccggaa tacaacctct gttgacctga agcgcttacc ggatgatgtg gaagcaaccc
     3361 gttctgttgt ggaatcggcg ctgacagaag gtgccattgg ttaccggaaa ttcagcgtgc
     3421 ttaaagggaa acgtctgttt gcaatactgc gtcttgctga tggctctcag cccccgtttg
     3481 gtgccagtgt aaccagtgaa aaaggccggg aactgggcat ggtggccgac gaaggccttg
     3541 cctggctgag tggcgtgacg ccgggggaaa ccctgtcggt aaactgggat ggaaaaatac
     3601 agtgtcaggt aaatgtaccg gagacagcaa tatctgacca gcagttattg cttccctgta
     3661 cgcctcagaa ataaagtaag cgactcgtca gaaccgtatt gatatttact gagagctcag
     3721 atcaactttc cagggcaaca gatcgcgtac ccggtttgcc ggccagtcct ggatatgttc
     3781 aatgacgtaa cgcagccact tcgaaagcac agcgggtacg ggtggaggtt ttttcaaaaa
     3841 tcagggcaat gtttttgcca accaaggtct gtttttctcg tccggctttt ttggccgctt
     3901 tcagtttgat cgcgagatcg atcaggtact ggatctctgc cggggtgtag tccagcagtt
     3961 taaggaagtt gcgttttttc aaattgatgg tcattaggaa atccttttta aattttaact
     4021 gtctgatgcc ctcactctgc catctcccct ttgaggagag ggttaggtga agggggattc
     4081 agttacgaat caatgtgcct ttcttgcccg ccaaaatctc agccccgtcg accagagcgc
     4141 cgatcccggc aataccgttg caagcttcaa caaacttgca gcaggcggcc actttcggcc
     4201 ccatcgatcc ggtgtcaaac tgcatgcctc tgagcagttc tggcgtcacc tgcgctagcg
     4261 gacgctgggt cggtttgccc caatcgaggt aaaccgcgtc ggcatcagtg agaatcagta
     4321 gtgcgtcggc ccctatctgg tatgccagca gggcagcaga gaggtctttg tcgatcaccg
     4381 cctcaattcc gcgatagccg ttagcctttt ccacaactgg cacaccaccg ccgccgttgc
     4441 agataacaag atggtcgcgc tggatcagtg ccgtaatagc atcgctctcg acaatgcgtt
     4501 tcggctgtgg agatggcacc acgcgacgga agtagctgcc gtcggcctta aacccccaac
     4561 ctttttctgc tgccagtgtt tttgccaggt cttcgttgta caccggtccg atatatttgg
     4621 tcgggttgct gaacgccggg tcagtagcgt ccacttccac ctgagtaagc aggacgctca
     4681 cctcacgctg tggcaggctg tttttcagcg cctgctggag catgtagccg atcatcccct
     4741 ggctttcggc gccaagaacg tccagtggat aaggggttac tttgtcgtag gcgctgttct
     4801 gcagcgccag cagcccgatc tgtggaccgt tgccgtgaac caacaccacg cgccaattca
     4861 ccgtgagccc ggcgatggta cgggcggcca actcaatgtt ctggcgctgg atttctgctt
     4921 ccagtggttc gccgcgcttc aataatgcgt tgccgcccaa cgccacaacc aaagtgggtt
     4981 ttcgttccat gatggctcct ttaaattccg tcgcgttcaa gcggacagct catgcagcgt
     5041 gcgccgccgc ggcctcgtcc caattcgtcc cccgggatgg gcagcacggt gatgccggct
     5101 ttgtcgtatt tctcgttagt ccagacgttg cgctcgtaac caatcaccac accggggcgg
     5161 atggtcagaa cgttattggc gtcgttccac tgctcacgtt cggcttcaaa ggcgtcgccg
     5221 ccggtggtga tcaagcgtac ctggtcaata ccgagtgctt tctcgatggc gtgcaacagg
     5281 tcggtttcct gggtccgttg taggccatcg cgtccgttcg aagtgagcgt ccagcactgg
     5341 gcgtctttgc gcaccacttc cgggtaaacg gagaaagtgt caacgtcgat gtgggtcatg
     5401 acggtgtcaa ggtgcataca ggagcggtgt tttggcagct caacggcgat cactcgctcg
     5461 gcttgacgat gtttgaacag gctgttggcg aggaactcca cgccctgcgg tgttgtgcgt
     5521 tcagacatgc cgatcaatac cgccccacga ccaatcacta atacgtcgcc accttccaaa
     5581 gtggcgtggt cgtaataaat attttcgtcg ccgaaatact taataaaatc gccgtcggca
     5641 aatgccgggt gccagcgata tattgcccgg aggttattgg tttcacgttg acgggctggt
     5701 ttggccatcg ggttaataga aacaccgtta taaatccagc aggaggtatc gcgggtaaat
     5761 aaatgattcg gtagcggctt cataataaag tcattagacg tgtgggtatc caccaccata
     5821 ttgttaatgg cagccggaat ttcaccgtaa gttaatccgc cgcttaatct tcgcgccagt
     5881 tcacggtgcg gcatgtccgc cagccagctg cgcacatcgc ccgcaaaggt aggtccgagg
     5941 cggtagtcag aaatttgcgt ctccagtaac caagttttcg cttctttaat atcaagggtt
     6001 tgtgtcagga ggtctgttaa cagcaggact tccaccccct gatcgcgcag cgtatttgcg
     6061 aagatgtcat gctcttcacc tgcccgttca accgagagta catcatcgaa aagcagttcc
     6121 tgacaattcg atggtgtcaa acgtttcaga cttaaatttg ggcggtgcag cataacacta
     6181 cgcaattgac caatttcaga accgacgtaa tgcttttcca taattattcc tttaattttt
     6241 tcagaataaa aaggtatgcg ccatgttgtg tatttaattt catggcggct aagttcaatt
     6301 gatttcgtgg ttcatactag gcggtaaaat aaacattatt gtgatgagat tcacacaaga
     6361 atggatatta aggtgtttgt tttaatttgt gttatttgca tcaaataatg attaatttca
     6421 taatgtcagc tcatgaatag ataatcatta tttgggtgga aatatttcta tttgttatta
     6481 tttt
//
LOCUS       DAJOTB010000215         6481 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJOTB010000215 DAJOTB010000000
VERSION     DAJOTB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.215"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            204..1574
                     /gene="aroP"
                     /locus_tag="OHN28_004508"
     CDS             204..1574
                     /gene="aroP"
                     /locus_tag="OHN28_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCQ2299776.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1617..2471)
                     /gene="ampE"
                     /locus_tag="OHN28_004509"
     CDS             complement(1617..2471)
                     /gene="ampE"
                     /locus_tag="OHN28_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCQ2299777.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2468..3019)
                     /gene="ampD"
                     /locus_tag="OHN28_004510"
     CDS             complement(2468..3019)
                     /gene="ampD"
                     /locus_tag="OHN28_004510"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCQ2299778.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3107..4000
                     /gene="nadC"
                     /locus_tag="OHN28_004511"
     CDS             3107..4000
                     /gene="nadC"
                     /locus_tag="OHN28_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCQ2299779.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4204..4644
                     /gene="ppdD"
                     /locus_tag="OHN28_004512"
     CDS             4204..4644
                     /gene="ppdD"
                     /locus_tag="OHN28_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCQ2299780.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4654..6039
                     /gene="gspE"
                     /locus_tag="OHN28_004513"
     CDS             4654..6039
                     /gene="gspE"
                     /locus_tag="OHN28_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCQ2299781.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6029..>6481
                     /locus_tag="OHN28_004514"
     CDS             6029..>6481
                     /locus_tag="OHN28_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCQ2299782.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKA"
BASE COUNT         1608 a         1731 c         1658 g         1484 t
ORIGIN      
        1 ttttttttta tccagaaaat caacacatct acataaagca ggtgcattct cgcgcgtata
       61 ccattatctt gtgatagttg aaagtactat catcataatt cagttacata aaatgtaaag
      121 aaaccaatac aacaaacgga attacaaaat tacacacgca ttgctgcgta gttcaaaaaa
      181 acaacacaac acgaggtttc ataatggaag gtcaacagca cggcgagcag ctaaagcgcg
      241 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat
      301 tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
      361 ttgctggttt tatcgccttt ctgatcatgc gtcagctagg tgaaatggtg gtcgaagaac
      421 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
      481 cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag ctgactgccg
      541 tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtgt
      601 tctttgtggt gatcaacgcc atcaacctga caaacgtaaa agtgtttggc gagatggagt
      661 tctggtttgc cattatcaaa gttatcgcgg tggtagcgat gatcatcttc ggcgcctggc
      721 tgctattcag cggcaacggc ggcccgcagg cgagcgttag caacctgtgg gatcagggtg
      781 gtttcctgcc acacggcttc accggtctgg tgatgatgat ggcgattatc atgttctctt
      841 tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
      901 tcccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggctcgttag
      961 ccgttctgct ctcactaatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
     1021 tgatcttcca cgagttaggc gatacctttg tagcgaatgc gctgaatatc gtggtactga
     1081 ctgctgcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgttcggtc
     1141 tggcacaaca agggaacgcg ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg
     1201 ttaacactat tctggtgtct gcgctggtta cggcattgtg cgtactgatt aactaccttg
     1261 ccccagagtc cgctttcgga ctgttaatgg cgctggtggt atctgcactg gtcatcaact
     1321 gggcgatgat cagtctggcg catatgaaat tccgtcgtgc caaacaagaa caaggcgtgg
     1381 taactcgctt ccctgccctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
     1441 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
     1501 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
     1561 taaaagcgca ttaatctctc tacgccctca cccgtgcagg gtgagggcaa taatctttac
     1621 accaacgccc cgtaaatcgt cagtagcgca attaccacca cgaccacgaa cgaggttttc
     1681 ttcgccattg aaaccgcggc ctttggcgtc tccaccttat cgacatgcgg ttcacgcgcc
     1741 agagagaact gtgccagacg cgttaatacc tgatattgcg aagtatggaa atcacccagc
     1801 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
     1861 gcaagacgaa ccggcaccca atccagtgca tgaagcacgg catcaatgcc ggactgtaaa
     1921 cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa aaacgcatac
     1981 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggagcaaga
     2041 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttca
     2101 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcatgggca
     2161 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
     2221 gcgccaatac acagcaaacc aatcagcagc cacaccagca acgtgggaac gttgaacaat
     2281 acaccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat ggtcatgcat
     2341 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
     2401 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
     2461 aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag
     2521 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga
     2581 tatccggata gcgatcaatc agtgcccgtg taaccgccgc aagctgttga tactgcgcat
     2641 cggtatacgc cagcgtatcg gtgccttcca gctcaatccc aatagaaaaa tcattgcagc
     2701 gttcgcgccc ctgatactga gagactcccg catgccaggc tcgtttatcg aaaggaacat
     2761 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
     2821 tctccgcaaa gaaaggatgc gcctgcggat caatagttcc agtgaataat gcgtcgatcc
     2881 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
     2941 tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccagccagcc
     3001 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
     3061 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
     3121 ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
     3181 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat
     3241 tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acagtgatca cccgcgagaa
     3301 tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
     3361 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc aacccttgtt
     3421 cgaacttgaa ggcccatctc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
     3481 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac tggaaggcac
     3541 caacacgcag ctgttggata cgcgcaaaac cttacccggc ctgcgttcag ctctgaaata
     3601 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
     3661 caaagaaaac catattattg cctccggctc agtgcggcag gcggtcgaaa aagcgtcctg
     3721 gttgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaagc
     3781 cctgaaagca ggtgccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
     3841 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
     3901 aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgttggtg cactaactaa
     3961 acacgtacag gcactcgacc tttcaatgcg ttttcgctaa ttgctaacag atttatccgc
     4021 tccatcgcgg gcggataaat ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt
     4081 cttgtctttc gctgcaacga agtaaaaaac ctcggaagct gctttcccgt ttcgtttttt
     4141 gcccctcgct tgtgctgatg gcgtttgcca aagtagcgcc aaccaaatca aggagcgaaa
     4201 cagatggaca agcaacgcgg ttttacactt attgaactga tggtggttat tggcatcatt
     4261 gccattttaa gcgccattgg tattcccgct tatcaaaact acctgcgcaa agccgcactc
     4321 accgacatgc tacaaacctt tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa
     4381 catggtggat taaatacctg cgacggtggc agcaatggca ttccttcgcc taccaccacc
     4441 cgctatgttt cagccatgag tgtggcaaag ggcgtggtgt cgctgaccgg gcaagaaagt
     4501 ctcaatgggc taagcgtcgt catgacaccg ggttgggata acgcaaacgg cgtcaccggc
     4561 tggacgcgta actgcaatat tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc
     4621 ttccgctttg atgacgccaa ctaaggagcg gcaatgaata ttccacagct cactgccctg
     4681 tgtctgcgtt atcagggagt cttgctggat gccagcgaag aggtggttca tgttgcggta
     4741 gtcgatgcac cttcgcatga gctactggac gcattgcatt tcgctaccac caaacgtatt
     4801 gagatcacct gctggacgcg ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca
     4861 ttgcccgtag ctgttcagga gaagcatcag cccaaagcag agttgctgac tcgaacgtta
     4921 caatctgcgc tagaacaacg cgcgtctgat attcatatcg aaccagcgga caatgcctac
     4981 cgcatccgct tgcgtatcga cggcgtattg catcctttac cggacgtttc accggatgcc
     5041 ggagtcgcat taaccgccag attaaaagtg ctgggaaacc tggatattgc ggaacatcgc
     5101 ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa acgccgtctc atttcgtatt
     5161 gcgaccttag catgtcgggg tggtgaaaag gtggtattaa ggttgttaca gcaggtgaac
     5221 caggcactgg atgttaacac gcttggaatg cagccgtcac aactggtgga ctttgctcat
     5281 gccttgcaac aaccacaggg actggtgctg gtaactggcc ctacaggcag cggcaaaacg
     5341 gtcacgcttt atagtgccct gcaaacgctg aataccgctg acattaatat ttgtagcgtc
     5401 gaagatccgg ttgagatccc catagccgga ttaaaccaga cgcaaatcca ttcgcgtgcc
     5461 ggactcacct ttcagggcgt tttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg
     5521 atcggagaga tccgcgatgg cgaaacggca gaaattgcca taaaagccgc gcaaaccggt
     5581 cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt acgtttacag
     5641 caaatgggag tcgcccgctg gatgctatca tcggcgctta cgctggtaat agcccagcgt
     5701 ctggtacgca aactttgccc gcattgtcgc cggcagcaag gggagcccat ccatattcca
     5761 gacaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt acattgctac
     5821 cacggttttt atggtcgcac ggccttattt gaagttctgc ccataacacc ggtcatacgt
     5881 cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcccg acaggcgggt
     5941 atgcgaacgc tttttgaaaa cggctgcctg gctgtggagc aaggcttaac cacctttgaa
     6001 gagttaatcc gcgtattggg gatgccgcat ggcgagtaag caactctggc gctggcgtgg
     6061 cataaccggc gacggcaatg cgcaagatgg gatgctatgg gcagagagcc gtgctttgct
     6121 gctcatggca ctacagcaac agatggttac cccactaagc ctgaagcgaa tcgccatcaa
     6181 ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg cgacgctact
     6241 caaagccggg ttaacgcttt ctgaagggct ggctctgctg gcggaacagc atcccagtaa
     6301 gcagtggcaa gcgttgctgc aatcactggc gcacgatctt gaacagggcg ttgccttttc
     6361 caatgcctta ttaccctggt cagaggtatt tccgccgctc tatcaggcga tgatccgcac
     6421 aggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc agcaaaaagc
     6481 c
//
LOCUS       DAJOTB010000216         6448 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJOTB010000216 DAJOTB010000000
VERSION     DAJOTB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.216"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            22..894
                     /gene="pgrR"
                     /locus_tag="OHN28_004515"
     CDS             22..894
                     /gene="pgrR"
                     /locus_tag="OHN28_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCQ2299783.1"
                     /translation="MAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTR
                     TTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVEHAAKTILLP
                     AMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRIGPDIPMAIV
                     GSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNT
                     IDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSA
                     FSLFIDRLKYKGAV"
     gene            1231..2844
                     /gene="mppA"
                     /locus_tag="OHN28_004516"
     CDS             1231..2844
                     /gene="mppA"
                     /locus_tag="OHN28_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCQ2299784.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2895..3926)
                     /gene="ynaI"
                     /locus_tag="OHN28_004517"
     CDS             complement(2895..3926)
                     /gene="ynaI"
                     /locus_tag="OHN28_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCQ2299785.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            4170..4427
                     /locus_tag="OHN28_004518"
     CDS             4170..4427
                     /locus_tag="OHN28_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCQ2299786.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(4477..5427)
                     /gene="uspE"
                     /locus_tag="OHN28_004519"
     CDS             complement(4477..5427)
                     /gene="uspE"
                     /locus_tag="OHN28_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCQ2299787.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5579..6331)
                     /gene="fnr"
                     /locus_tag="OHN28_004520"
     CDS             complement(5579..6331)
                     /gene="fnr"
                     /locus_tag="OHN28_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCQ2299788.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT         1740 a         1456 c         1529 g         1723 t
ORIGIN      
        1 cgtgaagaaa tcgctgatct gatggcgttt gtcgttgttg cagaggagcg tagcttcact
       61 cgcgcagcag cccgcctgag catggcgcag tcagctttaa gccagatagt gcgccgtata
      121 gaagaacgat tgggattgcg gcttctgacg cgaaccacgc gcagcgttgt acccactgaa
      181 gcgggcgagc atcttttgtc tgttcttggc cctatgttgc atgacataga ttctgccctg
      241 acatccctga gcgatctgca gaaccgccca tccgggacaa tacgtattac tactgtagaa
      301 catgcagcaa aaacgatatt gttaccagcc atgcgcacat tcctgaaatc gcatcctgaa
      361 attgatattc agcttaccat tgattatggt ttgaccgatg tcgtttctga acgttttgat
      421 gcaggcgtcc gtctgggtgg ggagatggat aaagatatga tcgccattcg aatcgggcca
      481 gacataccaa tggctattgt tggctcaccg gattattttt ctcgtcgaag tgctccaacg
      541 tcagtgtcac aattaataga tcatcaggca attaatttgt atcttcccac atcgggtaca
      601 gcaaatcgct ggagattaat acgcggtgga cgtgaagttc gtgttcgcat ggaaggtcag
      661 cttttactga atacgataga tctgatcatt gatgctgcaa ttgatgggca tggattggcg
      721 tatctacctt atgatcaggt ggagcgggct attaaagaaa aaaaactgat acgtgttttg
      781 gataaattca caccagattt acccggttat cacctgtact atccacaccg tcgacatgct
      841 ggctcggcat tctcattatt tatagatagg ctgaagtata aaggtgctgt ttagcactac
      901 ttgctgatac attaatttaa ttcttctctt aacgtattct cagttccttt caacgttttg
      961 gttatttttt attcttcgca caatggcgac agatgctgat tatgataccg aaaacgggtt
     1021 tgaatgtgcg aagcccgaac gtagtgttcg gagttctatg tgctttaccg cattttggag
     1081 actattattt acactaaatc tgatttgata tattgatact taaaacattt gatgcttcct
     1141 ttgtcacttt tttgatggaa gttgtttgca tttctttaag gcgaaacaaa taattacgca
     1201 tcaattttaa tgtcggttag agggaaactt atgaagcact ctgtttcagt cacgtgttgt
     1261 gcgctgttgg tcagcagcat ttctctttcg tatgctgctg aagttccgag cggcacagta
     1321 ctggcagaga agcaggagct ggtgcgccac attaaagatg agccagcgtc gctggatccc
     1381 gctaaagccg tgggcctgcc ggagattcag gtcattcgcg atctgtttga aggactggtg
     1441 aatcagaacg agaaagggga gattgtcccc ggcgttgcga ctcagtggaa aagtaatgac
     1501 aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg cacaccggta
     1561 acggcgcaag attttgtcta cagctggcaa cgtctggtgg acccgaaaac gctttcgcca
     1621 tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat tgatggtaaa
     1681 gccacgcctg accagcttgg cgttaccgca gttgatgccc atactttgaa aatccagctt
     1741 gataaaccgt tgccgtggtt tgtgaattta accgctaact ttgccttctt cccggtgcaa
     1801 aaagccaacg tagaaagcag taaagagtgg acgaaacccg gaaatctgat cggcaatggc
     1861 gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg tcgtagtgcc gaatacccat
     1921 tattgggata acgccaaaac ggtactgcaa aaagtgacct tcctgccaat taatcaggaa
     1981 tccgcagcca ctaagcgtta tctcgcgggg gatattgata tcaccgaatc cttcccgaaa
     2041 aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc gccgcagctc
     2101 gggacctatt attatgcgtt taacacgcaa aaagggccga cggcagatca gcgcgttcgc
     2161 ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtgtt ggggacgggc
     2221 gaaaagccag cgtggcattt tacaccagat gttaccgcgg gatttacgcc ggaaccttcg
     2281 ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt gttgagcgcc
     2341 gctggttatg gtccgcaaaa accgctgaag ctgacgcttt tgtataacac ttcagaaaac
     2401 catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaacctggg cgtagatgtt
     2461 aaattgcaaa accaggaatg gaaaacctat atcgatagcc gtaataccgg caattttgat
     2521 gttatccgcg cctcgtgggt aggggattat aatgaaccct ccactttcct gacattatta
     2581 acgtcaacgc attccgggaa tatttcacgc tttaacaatc cggcatatga caaagttctg
     2641 gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa cgcggcagaa
     2701 aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa tggacgatta
     2761 atcaagccgt ggctgaaagg ttatcccatt aacaaccctg aagatgtggc gtacagtcgg
     2821 actatgtata ttgtgaagca ttgatgtgat gagaactggc gttacccttg tggataacgc
     2881 cagtgatgtc tgatttagcg accctggtcc gccggtgtaa tgttatccat atacagcgtc
     2941 tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat cttcaaataa
     3001 acgtcttgct gtgcagcaag ccattcagcc cataccgtgg ttttggtaaa gcaataaacc
     3061 ataatattta atgacgaatc agcaaactgg ttgaaataaa ccagtaaggt ttgtcgctgg
     3121 tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac gcccactttt
     3181 gccgcatcct cataacgtaa accaatggtc gtggtaatgc gccggttggt cattcgtcca
     3241 ggattttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg acgattatca
     3301 aaggtcttaa ttttggtaat tcgccagcca atttccgcta ctgtaccttc gatatttctg
     3361 tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag cataatcccg
     3421 gaaaagaagt tactcagaat atctttaccg gccataccga cagccagacc accaatacca
     3481 ccaaaggtca acaagccaga aaggctcatg ccgaaatgtt cgccataaag cagaacaagc
     3541 accacaataa tggtgatttt gataatgcgc gacatgatcc gcgcactggt gatatcgcga
     3601 ccttttttaa tctgctgttt ttcaaactga ttaatcagca gaaatagctt aatcgtcaga
     3661 ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggggtgat aaatttgagt
     3721 ttatagtttt ctataacata gttaatgatg ctaccaacag cactaataat tatggtgtag
     3781 attaaaaatt gcaccgcatg gaataaaaat ccttttcttt tacgatttcc acggcgaaac
     3841 caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag attaagcgca
     3901 ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata attgtcgtca
     3961 tgccggaaac ccctggcggg actattttac cgcgacaatt cattcagatc atcaatagtc
     4021 agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa tccagctaag
     4081 aggtgaggtt ttcagagcag acaacggtga aatgtcatgg tattgttacg tttaggtaac
     4141 aaaaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag tcagcgaaag
     4201 ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc
     4261 gcgcgaccat cggcggcgtt atagaacagt acaatattcc gctgtctgag tggacgacat
     4321 caatgtatgt gattcagtca tcgatgattt ttgtttatag cctggtcttt actgtgttgc
     4381 tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta aaaaataggc
     4441 ccgataactc gggccttgtc agttattgaa gagtcgttaa tcgtcttctt cgtcatccag
     4501 ttcaacgggt gtctgatact ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc
     4561 aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt
     4621 gcccagaacc acaatccccg cctgtaagtg ctccgccaaa tcaggaatca cctcttctgg
     4681 cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgtc gcagagcttt
     4741 cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcgggt caaattccgg
     4801 cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat gaacttcggt
     4861 atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat tgagcgcatt
     4921 atggtacggc tcttcactgg cgagattcac cgccaccagc gccttacccc cttccggcca
     4981 cggctggtct ttcaccatcc acacggggct tgggcatttg cgtaacagat gccagtccgt
     5041 tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta gcaccaaatc
     5101 gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat gccagaccac
     5161 tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt ggatccaggc
     5221 tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccggag agagcagggt
     5281 ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc caccaatccg
     5341 ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag gatcgataac
     5401 aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg tcaacgcagt
     5461 ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc ttctgtcaga
     5521 tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg cggaagaatc
     5581 aggcaacgtt acgcgtgtga ccagcaagct gggccagcgc atcgttattt tcgatagtga
     5641 tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc agacggctga
     5701 tggtttcaac cgtcaggccc agatagttac cgatatcacc acgagtcatc gtcaggcgga
     5761 attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag atgaatgcag
     5821 ccagacgttc ctcggcattt ttcttcgaca acagcaggat catgtcctga tcgcctttga
     5881 tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta ccagacaaat
     5941 cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc tgcgcgaaac
     6001 tcgggtgatg accgctgccg atggcatcaa atcccaccag atcgcctgct aaatggaaac
     6061 cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta ccggagcgga
     6121 tggcataaag cgatttaagt tcatctccag ccttaaacag cgtctggcct ttctgaatag
     6181 gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg agtgtgaacg
     6241 ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg ccagactgaa
     6301 tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa ttgatatttg
     6361 tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac agcagagaat
     6421 tccgatatta gatgtaaata tatgtcta
//
LOCUS       DAJOTB010000217         6415 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJOTB010000217 DAJOTB010000000
VERSION     DAJOTB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.217"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..442
                     /locus_tag="OHN28_004521"
     CDS             <1..442
                     /locus_tag="OHN28_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299789.1"
                     /translation="LFQRARDDQNAGCQTDYVHAAIIADQMMSNASELRGLHGDLHHE
                     NIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIAQMADAFSRALDV
                     DPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            660..1475
                     /locus_tag="OHN28_004522"
     CDS             660..1475
                     /locus_tag="OHN28_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2299790.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1633..4518)
                     /locus_tag="OHN28_004523"
     CDS             complement(1633..4518)
                     /locus_tag="OHN28_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCQ2299791.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            4644..5267
                     /locus_tag="OHN28_004524"
     CDS             4644..5267
                     /locus_tag="OHN28_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2299792.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRD
                     DRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQID
                     TTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
                     AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGGLASVSFRSF"
     gene            5298..>6415
                     /locus_tag="OHN28_004525"
     CDS             join(5298..5544,5544..>6415)
                     /locus_tag="OHN28_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611355.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1133 family transposase"
                     /protein_id="HCQ2299793.1"
                     /translation="MSLKHSDEFKRDAVRIALTSGLTRRQVASDLSIGLSTLGKWIAS
                     ISDETKIPTQDTDLLRENERLRKENRILREEREILKKAGNIFRSTKAVRFQFITDYRG
                     SLSRSRICRLMGVTDRGLRAWKRRPPSLRQRRDLILLAHIREQHRLCLGSYGRPRMTE
                     ELKALGLQVGQRRVGRLMRQNNITVVRTRKFKRTTDSHHTFNIAPNLLKQDFSASAPN
                     QKWAGDITYVWTREGWVYLAVILDLYSRRVIGWATGDRLKQDLALRALNMALALRKPP
                     PGCIQHTDRGSQYCAHEYQKLLLKHQLLPSMSGKGNCFDNSAVESFFKSLKAELIWRR
                     HWQTRRDIEIAIFEYINGFYNPRRRHSTLGSDESPNDFG"
BASE COUNT         1429 a         1719 c         1816 g         1451 t
ORIGIN      
        1 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
       61 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
      121 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
      181 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
      241 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
      301 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
      361 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
      421 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
      481 atcttaccgg agaatatcgt tggccaaagc cttagcgtag gatttcgccc tctcccgcaa
      541 acgaccccgg gaaccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
      601 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      661 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacctggatg
      721 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      781 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      841 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      901 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      961 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1021 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1081 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1141 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1201 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1261 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1321 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gaattttctc
     1381 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1441 tgcagtttca tttgatgctc gatgagtttt tctgatagtt agtctttgta gagacacaaa
     1501 cctgaaattc cggacaccgc gcttcaggac atcgctcaag acgcctatat cgggcagtat
     1561 ccggacggta ctatcgaacg ccggtaccct gccctttatg gggtcgtttg cgggaggggg
     1621 cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca
     1681 tccgagcggc gaaacatggg ccaagagatc gggcgatagc agctttccat cgcgtttctg
     1741 gtttgcaacg acctcgccga gcttcatggt gttccagaag atgatgatgg cggcgagcag
     1801 attcatgccg gcgatgcggt aatgctggcc ttcggcggaa cggtcgcgga tttcaccgcg
     1861 gcggtggaag ctgattgccc gcttcagcgc atgatgagct tcgcctttgt tgagcccgat
     1921 ctgggcacgc cgttggagtt cggcatccag aatccagtcg atcatgaaca gggtgcgctc
     1981 gacgcgaccg acttcccgca gggctgtcgc gagctcgttc tgccgcggat aggaggcgag
     2041 tttccgcaga atctggcttg gcgcgacggt cccggcagca atggtggcgg cgatgcgcag
     2101 gatgtcgggc caattgcgct cgatcatggc ttggttgacc tttccgccga tcaacgctcg
     2161 caggtgcgcc ggggcggccg acggattgaa cgcgtagagc cgtttggatg gcaggtcgcg
     2221 gatgcgcgga gcgaaccggt agccgagaat ggcacatgcg gcaaagacgt gatcggtgaa
     2281 gccgcccgtg tcggtgaact gctcgcggat atggcgtcca gcatcgttca tcagcaggcc
     2341 atcgaggatg taaggcgctt cgcttgccgt tgcaggaatc acctgggttg cgaacggcgc
     2401 atattggtcg gagacgtggc tataggcttt caggcccggg gtattgccat atttcgcgtt
     2461 gaccaggttc atggcctcac cttgctctgt agcgacgaag aactgtccgt cgctcgaagc
     2521 cgacgtgccc atgccccaga accgggccat gggtaacgct gcctgtgcct cgaccaccat
     2581 ggccagcgcc cggtcatagg cttcgccctc gacatgccac cgtccaatgc ggatcaattc
     2641 ccagaaggtg tgggtgtttg tcgcatccgc cattttgcgc aagccgaggt tgatcccttc
     2701 cgccaagata acgttcatta gcccgatccg gtcagcgcag ggtgctcctg tgcgcagatg
     2761 ggtgaacgct tcggtgaagc cggtcgccgc atccacctcc agcaggagat cggtgatgcg
     2821 cgtgggcggg atctgcttgt agagatcgag caccagatct tcggcgcctg tcggcgcggc
     2881 ggcttcgagt ttctcgatat gcagaacgcc gttttcaatc gacccgcccg ggatcgtgcc
     2941 tgcgcgagcg gcacggccaa gctcgcgcaa ccgcatgtcg aggcgagctt gccggtctgc
     3001 cagccattcc tccggccgca atggcacagc gagacgaccg ccttccgcga tggattgtgc
     3061 cggaacgagt gcgtgtttca gatcgccata gcgccgggac ctagtaagcc agacatctcc
     3121 ggagcggaac gcatcgcgca gatggaacag caccgcgatc tcccataggc gagcgtcgcc
     3181 agccctctgg gcccgaaggt ggcgatgcca tttcgagctg ggccgcaaga agctggtcat
     3241 cgcggcatcg ttcaaaccgg tacgaagggc cgtcaccgct tccagaagcg gcagtgcaac
     3301 gggcgcagct cgcagatcga gcaggcgcaa catgcgtgga gcgtatcggc ggaagcggtg
     3361 ataaccgtcg agcacatgat tgagcggatc gtcggccatg gtggcggtca gcctggttgc
     3421 cattgcaaca agggttttta agccgtccca ccctgaccca ctcgcgatga catcgcccag
     3481 cggctggcca tcatcctgtg catcgaccag ggcgcccccg atctcggcga aggatttcag
     3541 ggtgtcacgc accacccccg cttcgtctgc gacctttgca tggcaaatac gctccgaagc
     3601 acggtagaga cggccgacga tccggtcgtg ggtttcgacc actgcgtcgg ccaacatcgc
     3661 ctgccattcc gagacgcaaa cagccaagat cgcaagccgc ctgtcctccg ggagatcgcg
     3721 catgccgtcg gcataatacc gttcaccctg cctgcgcaga cgagtcaccc gatgggcagg
     3781 aacgccggca agcagatcct cggggagatc gatgcgttgc agatattcga gccggtcgag
     3841 cagccggttg gccgacgaag agttcgagcc aggctcgaac tggcgcagcc acacaaaacg
     3901 ggtcacccga tcatcagccg tctcctcgag caatgccagc aactgttctc ggatcgacat
     3961 aggcagccga ctggcgatcc tcgtctcgat gcgtcgctcg gcatcgacga gagccgcggc
     4021 acaaagccgc tcgatcgtgg atgtcgcggg aaggacagtg cgggtgcgtc ggcactcggc
     4081 tacgaagcga cgggcgatat cctcgttcga caccgccatc tcggcttctc ggaacaacca
     4141 ttccttcagc tcgctcgcac cacgtccgga gaaggtgcgg aagccgtaga gcccccgtaa
     4201 ctcggcaaga tgctcgtgcc gtgtttcctc gcgggcagca tagtctacga gatcgtcggc
     4261 acccaggcca agctgcgctc cgataaattc gatgacctct gcagggatca gttcgcctgg
     4321 agccagcacc cggccgggat agcgcaggac acacaattgc agggcgaagc cgaacctgtt
     4381 gtgagcgcgc cgacgcagcc tgatatgccc aaggtcttca tcactcagcg tatagtgctt
     4441 gagcaaatcc gtctgtgaag tcggcaagcg caacagcgcg tctttctgcc gatcggttag
     4501 agtgacgcga cgcggcatac atgttccttt ttcaaaatct gatagcgttc aagacgcttt
     4561 gtttatgaag ctggttgaga tacatttcca gaggtcaatg caatcgtggc cgaagcgccg
     4621 cctcaaacca acgtttgtga tacatgctga tcggatatgc ccgcgtctcc aaagccgatg
     4681 gctcgcagtc tctcgacctg cagcacgacg ccttgcgcgc cgcaggtgtc gaacgggaca
     4741 atatctatga tgatcttgct tccggcggtc gtgatgatcg ccctggcttg actgcctgcc
     4801 tcaagtcatt gcgtgacggc gatgtgctgg tggtctggaa gctcgatcgc ctcggacgat
     4861 cgcttgccca tctggtcaac acggtgaagg agctgtcaga ccgcaagatc ggcctgcggg
     4921 ttctgactgg aaagggcgct cagatcgaca ccacgactgc gtccggtcgc atggtgttcg
     4981 gaatcttcgc caccttggcc gagttcgagc gggatctgat ccgagagcgc accatggcgg
     5041 gtctcgcctc cgcgagagcg cgcggtcgca agggcggacg aaaattcgcg ctcaccaaag
     5101 ctcaggtgcg tctcgcgcaa gccgccatgg cccagcgcga tacttcagtt tccgatctct
     5161 gcaaggaact cggcatcgag cgcgtcactc tctaccgata tgtcggtccc aaaggcgagc
     5221 tcagagacca tggtggtctt gcctcggttt cgttccggtc tttttgagag cattatttgt
     5281 tatcaaggag accatatatg tcattaaagc atagtgatga atttaagcgt gatgcagttc
     5341 gcatagcact cactagtggc ttaacacgcc gtcaagttgc gtcagattta agtattgggc
     5401 tttccacgct tgggaaatgg atcgcatcaa tttccgatga aactaaaatt cctacccaag
     5461 acactgatct tctgcgtgag aatgaacgtt tacgcaaaga gaaccgtatc cttcgggagg
     5521 agagggagat attaaaaaag gcagcaatat ttttcgcagt acaaaagctg tgagatttca
     5581 gtttattacg gattaccgtg gctctctctc acgttcacgc atatgtcgtt tgatgggcgt
     5641 aacagatcgt ggtttacgcg catggaaacg ccgtcctcca tcactgcgcc agcgtcgtga
     5701 tcttatactt ctagcgcata tacgtgagca gcatcggttg tgtttgggga gctatggtag
     5761 gccgcgtatg acagaagagt tgaaagcgct gggcctgcag gttgggcagc gtcgggttgg
     5821 acgtttgatg cgccagaata acattacagt tgttcgaacg cgtaaattca aacggacaac
     5881 ggatagtcat cataccttca acattgcacc gaacctatta aaacaagact ttagcgcaag
     5941 cgcacccaac cagaaatggg caggcgatat cacttatgtt tggaccagag aaggatgggt
     6001 ctatcttgct gttatccttg acctgtattc ccgtcgcgtg attggctggg caacaggtga
     6061 tcgattaaag caggatcttg cattaagggc actgaatatg gcgttggctt tacgcaaacc
     6121 accaccgggt tgtattcaac acacagaccg tgggagccaa tattgcgctc atgaatatca
     6181 aaagctactg ctcaaacatc aattgctgcc gtccatgagc gggaaaggca attgttttga
     6241 taactccgca gtagaaagct tctttaaatc attaaaggct gagttgattt ggcgcagaca
     6301 ctggcaaaca aggcgagata ttgagattgc aatcttcgaa tatataaatg gcttttataa
     6361 tccacgccga agacattcaa cactcggctc tgatgaatcc cctaatgatt ttggt
//
LOCUS       DAJOTB010000218         6343 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJOTB010000218 DAJOTB010000000
VERSION     DAJOTB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.218"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..924
                     /locus_tag="OHN28_004526"
     CDS             <1..924
                     /locus_tag="OHN28_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCQ2299794.1"
                     /translation="AEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKD
                     LKLSVPQHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIA
                     ALSGAAAYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKAL
                     ENAHQCACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHS
                     VITEQQDISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMET
                     DDNGMNVINKDELDDVLASSVVKFDDFSSQS"
     gene            971..2542
                     /locus_tag="OHN28_004527"
     CDS             971..2542
                     /locus_tag="OHN28_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HCQ2299795.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAELNHFSLHLDSVNSEIERGLLLREEVRRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            2539..2805
                     /locus_tag="OHN28_004528"
     CDS             2539..2805
                     /locus_tag="OHN28_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2299796.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            2945..3142
                     /locus_tag="OHN28_004529"
     CDS             2945..3142
                     /locus_tag="OHN28_004529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299797.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRYLLSRKKTS"
     gene            3208..4881
                     /locus_tag="OHN28_004530"
     CDS             3208..4881
                     /locus_tag="OHN28_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2299798.1"
                     /translation="MYSSATVCFTVSAGQKYRFYSIIMKINLPFIHWSRISNDKRLVL
                     SVVVFTLSNILVSSGAHLIYRILFFTLALLIIKAMKSLTLRFILAFSFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARADNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSVNTVLHHDVRHYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGGRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNEPLE"
     gene            complement(5025..6068)
                     /locus_tag="OHN28_004531"
     CDS             complement(5025..6068)
                     /locus_tag="OHN28_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2299799.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDIWFGPLLGEFEELLDAYDTDEISE
                     TSYINKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHTSIQHR"
BASE COUNT         1719 a         1302 c         1540 g         1782 t
ORIGIN      
        1 gctgaagctc agacatcatc aaaattaaca acgctcggaa ataaagagct tgagattggt
       61 aaagatttca gtgagttcga caggattgct gctgagctcc tggcgcgtct ggaaaaagag
      121 ggcaataaag acctgaaact gagtgtcccg caacataaca tcaataagat tcagaacctg
      181 gccatttcag cgacagaata tctcagtacg gtcgttgctg ccggagtttc cggggctgct
      241 gcgggttttg ctgtgtatag cggagtgatg gcatttgctg cggcttctac aggtacaccc
      301 attgcagcgc tttctggtgc cgcggcttat aacgcgacga tggcggcaat tggagggggc
      361 tctctcgcgg cagggggcat gggaatggcc ggcggcgcaa tggtattagg caccgcagtg
      421 gctgcaccgt tgattgccat tgcgggctgg gcttacgaca gacatgcgac gaaagctctt
      481 gaaaatgccc atcagtgtgc ctgcgatgtt gcacgggcag ttaagaaaat gaatctggcc
      541 cgggagcatt ttgcaaaagt taatcaatac gttgatgaaa ttctcgctgc actgaacaga
      601 atgcatgctg tatttattaa acactatttt gagccgctta aatccatgca ttctgttatt
      661 acagaacaac aggatatcag tgtatcagat gaagtcattg tgcttattga ccatggatat
      721 tcactggccg ctattatgac ggatattatt acaacgccac tgtttaaacc gcggaaacag
      781 gaaaatggtg aggcagtgat aaaagataac gttattgaaa tggaaacgga tgataatgga
      841 atgaacgtta ttaataagga tgagctggat gatgtactgg ccagttcggt tgtgaaattt
      901 gatgattttt catcccagag ttaatgattt accgggcgtt aaagcccggc tttacaagaa
      961 gtgaaataac atgaatgtgg aaaaatttga ctggacagaa gagttacatc agaccatggt
     1021 gaagagcctg gttacatcat tcgggctgga ttttctgttg ctaaatgatc gtaagggtgg
     1081 tgatgttgac actatccata atgtgcgtaa tggcatttat gccacggagg ctgagcgaca
     1141 gcggtatgaa ggtcgtggcg aatatgattc tcatcattac cacagccatg agaactatat
     1201 cgcgacgaac cgggaaggaa agcgtgcgca tcaggcaggg actcttactg atgcatatac
     1261 aggcgaggtg tttgcttctg acgacaaaaa aaatctggat catattattt ccgctaaaga
     1321 aattcatgat gatccgggga gaattcttgc tgaacgggat ggtgcagaac tggcaaatga
     1381 cgcttcaaat ctggcattca ctcatgagtc gctcaataaa tcaaagaaag cagactcaat
     1441 ggatgcgttt atttgcacgc tgcagaaaaa cagagaagat acgttaaggc agatagcaga
     1501 gcttgaatct caggcaacgc tgacagaaaa ggaaaagaaa cgtctgataa gtctgagaaa
     1561 aaaagctgat gcagatactg agcgtatgga aagggctgat aaatatgccc gggaaaaata
     1621 tgaggccacg attaatcaga gctattacct gagtagtaag tttgctactg ctgtcgccag
     1681 tgcttcgctt aattccggtt ttcgtatggg gacgcgacag atgttggggc ttattcttgc
     1741 cgagatctgg ttcgaactga gggaacgaat cccgcatatt atcaggcagc aacggaactc
     1801 attcagcgcc ggcaaatgcc tgtccgaaat aacgtcagca ttacgtgcct gccaggaacg
     1861 agtacaggaa aaattcagtt cttttttaat tgcctttaaa gatggtgtca tcggagggat
     1921 actgtcaggt attaccacaa cgctgttaaa tatattcctg acaacggaaa gaatgattgt
     1981 ccggcttatc agggaaatgt ggaacaatct cgttcaggcc tttaaagtcc tggtatttaa
     2041 tccggaggga ttatcgccgg ggcagttagc caaagcggtc actaaactga tagcagcagg
     2101 tgtggctgtt gccggagggg tgatgataaa tgaggcgatg gcgcagatcc ttattttccc
     2161 atttggttca gagcttgctg ccttttgtgg tgcgctgacg acaggtattc tcgggctggt
     2221 gatgaattat tatctcgaat atagcgaggt tatgcagcgg gtatgggcat ttctggacaa
     2281 atttaaggac aaattccagc ggtcactgga atactttcag gcagtcaatg ctgaacttga
     2341 tcgctatctt gtggaactta ctgcactgga attttctgtt gatgctgccg agttaaatca
     2401 tttttcactt catctggata gcgtaaacag tgagatagag cgcggacttc tgctgagaga
     2461 agaagtccgc cgacgaggga ttgcgttgcc gtttgagtca ggaaatgtgg cttctgttcg
     2521 tgactggtta aataagcgat gaaattacac cctccatttc cctgtaacca gtgtggggcc
     2581 tgttgtcgtc atgtcaacag ggcgggtgaa acgcaatgtc tggatcgtgg tgacggtatt
     2641 tgccgtcact atcagactga ttctcatttg tgtgcaattt atgataaacg cccacagatt
     2701 tgccgggttg aagaccagta tttactgaat tatcagtctc agtacagctg gcaggagttt
     2761 attgcattaa accaggccgc atgccttatt ctgaataaac tgtaatgcca ttaatggcgg
     2821 cgtcaggaca gaaactgaga gaacataaaa cagtaaaatg tcataccatt cagcagccag
     2881 cgttactttc cccggctgct gttattgtaa ggcattttcg ttaagaatac agaaggagag
     2941 gagcgtgaga ttgctgttaa aagggctcgt cggaatggcg ctggcctggc ttgttattgg
     3001 tttcgggatg gagttatttt ttctcgccga tcgggttcat ccggcattaa gagtaattgt
     3061 tgtcctgggg atgttattcc tgctgataat gattgcgggt ggtatgtgcc gttatcttct
     3121 gtcacgaaag aaaacatcat gacgatcctg tttacgccgc tacatttgtg gcggcggatg
     3181 atgcccgttt tatgattatt cagaaaaatg tattcatctg ccacggtatg tttcacggtc
     3241 agcgccgggc agaaatatcg cttttactca attattatga aaattaatct tccgttcata
     3301 cactggtcac ggattagcaa tgataagcgc ctcgtgttat cagtggttgt ctttaccctt
     3361 tctaatatcc ttgtatcctc aggagcgcat ctgatttacc ggatattatt cttcaccctg
     3421 gcgcttctta ttattaaggc gatgaaatcg cttacgctgc gttttattct tgccttttcg
     3481 ttcacattgc tgacagcggc agatatcagt atcagtttgt attcatggtg tacattcggt
     3541 acaacattta atgatggttt tgcaatcagt atccttcaga cagatcctga tgaagtaata
     3601 agaatgttca ggatgtatgt tgtttatgtt atcgcattta ttatcctctt tttattgttc
     3661 ttctgttcag caataaataa aacgtcctca ttgccatcgg aaaaggtcac ggtaattaca
     3721 tttttattgt taattacagt aacgttatat tcttccttcc agtttgcgtt gaagaaacag
     3781 tatcaaataa atgaagttga tccttacata gtcgcttccc ggtttgccac atatacccct
     3841 ttctttaatc tgaactattt tgcactggcc gcaaaggagc atcagagatt aatgacgatt
     3901 gctgatacca tacctcacta cgatctggtg attacggata acaacactga cgtatttgtt
     3961 ctggtgattg gtgaatctgc aagggctgat aacatgtcca tttacgggta ttcgcgccca
     4021 accacaccag agcttcagaa acagaaatca cgactgaaac tgttcactca ggcaatcagc
     4081 ggggcgcctt ataccgccct tgcggtaccg ctggcattat ctgtaaatac cgtgctgcat
     4141 catgatgttc gccattatcc cgataatatt atcaacatgg cgaatcaggc gggattcgat
     4201 acctggtggc tcagcgcaca atcggcgttc cggcagaacg gaacagctgt ggccagtatt
     4261 gccatgcggg ccagaaacag aatttatgtc agaggctatg atgagttact gctcccacac
     4321 ctggctgaag cactgaacag taatccggga ggcaggaaac tgattgttct gcatctgacg
     4381 ggcagccatg aaccggtttg cagtaactgg cccagagata aagccgtgtt taaaccgctg
     4441 gatacagaag aagtctgtta cgataattcc attcattata ccgacagttt gttggggcag
     4501 gttttcgcca tgctggaaac gcgccgggca tccgtcatgt atttttcaga tcacggtctg
     4561 gagtatgacc cgacaaaaga acatgcctat tttcatggtg gaataaaacc cagccagcag
     4621 gcttatcatg ttccgatgtt tatctggtac agcccggcgc tgggagagca tgtcgatcgc
     4681 cagacggtga acagcgtttt ttccactgcg tataatgatt atctgattaa tgcatggatg
     4741 ggcgtaacca gaccggctca accgaaaacg ccggaggagg tgattacccg ctggcaggga
     4801 aaatccgtgg tttttgatgc cagccataac gtgtttgact acaaagtatt acgaaagaac
     4861 tttaatgaac ccctggaata attcgttaaa gtaatttact acgctatttt ttcaaaggaa
     4921 actttcagaa aataacccca gggcgataac ctggggaagt ggggggctgt gttattactg
     4981 gtggataatt tattacttgt ttgaaagata cactaatgca atatctaccg atgctggatg
     5041 cttgtgtgtt tcattcctgc cgctgaaaac ggcaatatcc agggccagac atattcaccg
     5101 ttaatatttc tgcatttctg tacaatttcc tcagaaagtg tctcatcaat tctttcccgc
     5161 aacagcttct gctgtcttaa aatgctttcg catatttcga aatcatcttc ctgcatcagc
     5221 atttcggcgc atttaatgat ttctgcccgt tcatgcagca cagaggaatg gttatagagc
     5281 cagtttacaa acagcaatgc ttccggatat tgtccccata atggtgagta tgttgcataa
     5341 taatcttcgg cagtatccgg cccggcagaa tagttatgcc atacagcaag aggaaatgga
     5401 tgcagatttc cgcacagaat ttctgcgatg taagtatttg ccgcaaatcc gcgacgaaat
     5461 gcagttgcag cttttaccag ttccccatta agaaaatgca gaagaccaag ttcataccag
     5521 taaggtgaaa attcatcagc atgttttttc agtacactga aggctcgctc atgatcacct
     5581 gttcgtaaca gctctgaacc caacaaccag cgggcaccct gattatcttc aggattgtat
     5641 gccagtattt tatcagtaag cataaccgca tcctgatgac gctgtaagtg cacatttgcg
     5701 agaattgctg cataaagcgc gcgcagataa ggccggtttt cgggatgcat ccagatgatt
     5761 tcaccactga aactttccgg gatgagacga ttacctgccg ccagtccttt aagagcggcg
     5821 ttcagtgcct tacgcggggc attctgctcc agaaatgcat atgccagatg agcgtgtata
     5881 tcaataaagt caggctcctg ttgagcaagg cgtctgagtt tgttgatgta actcgtctcg
     5941 ctgatttcat cagtgtcgta tgcatcgagc agttcttcaa actcccccag taatgggcca
     6001 aaccagatat ccggaaatac gaaacgcccc tggttatcgt tcacttcaaa gcgtaatctg
     6061 tcgttcattt tctgagcctg taaaacggat gggacacaat ctgaaaagca taaaagttgt
     6121 gatgttgtgt atcaagtctt gtttttgtcc gggatgaatg ttgtttacaa tatcatcggc
     6181 agtggttttt catgagaatg aaaatttatc tttatctgca ttccggtgta ttgtgaattg
     6241 tcggctgaaa cagcatatta atcgattttt ttaggggaat ggtgctgaaa taacgacatg
     6301 atgatgattt atattacagg tatcaaaatt gttagcggtc cat
//
LOCUS       DAJOTB010000219         6321 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJOTB010000219 DAJOTB010000000
VERSION     DAJOTB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.219"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..837
                     /locus_tag="OHN28_004532"
     CDS             <1..837
                     /locus_tag="OHN28_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCQ2299800.1"
                     /translation="LLKSESTALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTGI
                     DNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHG
                     CELQGKRIGLVGYGNIGKILAKRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFM
                     QSHAIIVLLPFSSELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFA
                     ALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYFAGRKIKN
                     VL"
     gene            complement(1137..2513)
                     /locus_tag="OHN28_004533"
     CDS             complement(1137..2513)
                     /locus_tag="OHN28_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCQ2299801.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNVQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(2513..2941)
                     /gene="tssE"
                     /locus_tag="OHN28_004534"
     CDS             complement(2513..2941)
                     /gene="tssE"
                     /locus_tag="OHN28_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCQ2299802.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(2945..3481)
                     /gene="tssJ"
                     /locus_tag="OHN28_004535"
     CDS             complement(2945..3481)
                     /gene="tssJ"
                     /locus_tag="OHN28_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCQ2299803.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(3462..4541)
                     /gene="tssG"
                     /locus_tag="OHN28_004536"
     CDS             complement(3462..4541)
                     /gene="tssG"
                     /locus_tag="OHN28_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCQ2299804.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(4505..6265)
                     /gene="tssF"
                     /locus_tag="OHN28_004537"
     CDS             complement(4505..6265)
                     /gene="tssF"
                     /locus_tag="OHN28_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCQ2299805.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1443 a         1703 c         1699 g         1476 t
ORIGIN      
        1 ctgcttaaat cagaatccac tgcattacga gaatctattt gcgcggtcat cgccggaaaa
       61 gattgttatc aggcggacac gctatcattg ctgtctggtg tgaaaataat atcgagattc
      121 gggacgggga tagacaatat tgatcttcgt gcggcacagc aatccggtat tgttgtcaat
      181 aatgcggttg ggattaattc aaatgcggtt gcggaattta tcattggact tatcttcgcg
      241 agcatgagaa atatccctgg cagctatcat gcgatgcaaa atggctactg gggtgagtcg
      301 catggctgtg aattacaagg gaaacggatt ggcctggttg gctacggtaa tataggtaaa
      361 attctggcga aaaggttatc cggttttgat gttgagcttt tagcttttga caaacaaccc
      421 gactatcagg ttgctgacaa agctggagtt cagtttgtat caattgaaga tatctttatg
      481 cagtcgcatg ccatcattgt tcttttgcct ttttcttctg agctggagaa cttcattagt
      541 cataaatatt tgtcgatgat gcgcaatggt gctctgatca tcaatgccgc aaggggaaag
      601 ttgctggatg agggcgcttt acttcaggtg attgaagaac gaaatgtgtt tgcggcgctg
      661 gatgtgttta gctctgaacc gttggcgcaa tttagcccac tgctacatgc caaaaatatc
      721 attacaacgc cgcatattgc ggccgccacc gttgagtcat accagcaaac cgggatacac
      781 gttgcccagt cgataattga ttacttcgca ggaaggaaga taaaaaacgt gttgtgagtt
      841 cagcgaacaa agattttaaa tacaaaaaaa ccgcaatttg tagaacacat tgcggtttgt
      901 cagcattcta ataaattggt tgcgggggcc acagtcaaga ggttaatcca atcttttgaa
      961 acttaaggta ttcactgttc ccgaacccaa gctatctacc gaaatataca ctcattatct
     1021 gatgtgaatc aacgagtaga tcgaatggcc tgttcctctc ttttttgtac acaaagactc
     1081 cttttaaaaa atagcaaggt cacatatccc ttattggtac ataaatcccc cgatgtttac
     1141 tgactttgtt ccaccttcgt atccagtaaa atgacatagc gattcagcaa ctgatttaac
     1201 cgggtatcaa tctgcgtcaa cagcgtcggc gaaatctctc cggtctgttt ttgctcttcc
     1261 agttgccgaa gcagttcttc cagcggcggc tcctttaacg actgccggat gccgtacacc
     1321 gccgttttta gttccgaacc ggtcagatat ctccccttac gttcatccag tgcatccagt
     1381 tgtgccgaca ggcgctgtag ctgactctgc gcctgtgcgt agccattcag tgcctctgcg
     1441 gacagggcgc tcagttcccg ctgtttacgc cactgcacgt tcagcgccct gacctgagga
     1501 ttatccggcc acagggcatc aagctggcgt agctggcgat atccctgctc cagcggccag
     1561 cgggcaggta atttgtttaa ctgctccagt tgcatctgac ttgccgcaat tgttctgtcc
     1621 tgagccagca gcgccttatc ctttacatcc agctttgcca gttcgccgct ctccagcgac
     1681 tgcggcaacg gcatgacaga cagttgtatt cgctggatta gcgcgtccgg ctgattaagc
     1741 tgcgaccagc cccataatcc tgttccgccg agaccggcca tcacaaccat tccggccata
     1801 aatatcggcc atgtccgctg tcgggagatt tcaggggatg acacgggtgg actggcatcc
     1861 ttatcgtttt gctgagcggg tctcgttgca aaaaccggct gtggactttc ttctgacttt
     1921 ttcggttgat tcatttcccg gcggggtaat tcagggggag tgatagtggt ttccgctccc
     1981 tgaggcgcca gccgttccag acgcaatgcg gtattgcgaa actgcccggc cagcacatcc
     2041 agtttcgtca tatgccacag ttcacactgt tgcagcgtgg tttcgatggc ggaaagatgc
     2101 tgtacacaac ggtagatctg cggcagatcc tgatactgaa tatccagcgt tctcagtgcc
     2161 tgctgcattt tttctgtgac ccagctgagc agtgatattc tggaatgtac ctgtactggc
     2221 cagaagtcag cccagcgctg gagcagtata tccagtgagc cgagtccgtc tgccatccct
     2281 gccagcccct gccgtctggt tatcgccagt acgtaacaga ccagcgtctg tagttccacg
     2341 ccattttgcc ggaacagcgc gaggcagagt ttttccacct gacgccagtc cacatccggt
     2401 cgggccgggt gagtcagttt actcatttca gcctgcagtt caccggattc cttataagta
     2461 cagggatcgc cgcccatgac gaactggctg ataaaattcg cgttacttgc catcagtacg
     2521 cccgctcctg ttgtttgaga tgacgcagaa caattcgtcc ttccggagaa aatacgccat
     2581 caaagcgtat ccagccggta tcgggaagct gcgcgtggat caggtaatta agatgccccg
     2641 gacggggctg ggggagtaac tccacctgta tctgactcag gcgtggttca taacgcagca
     2701 gcgtttcttc aatctgacgc atcaggccat gaatacccgc agccatcccc tgatggatca
     2761 gactgagatc cggcagaccg taatccggca aatgcgccag cgtgcctgcc cggcagttga
     2821 taatgcgttg catattgtcc atcacggaaa ggataagctg atcccgctct ctgacctgtt
     2881 ccagcggtaa ctcgccgctg aaactgaacg tcagcatttc atataaggaa ggtgtcgaca
     2941 tcggttattt atccttgaga ggcagcagtc gcaggtcgct tctcatcagt tcaatcgagc
     3001 gcggcttgtc ggcctccagt tcgtcccttt tgatcaccag acgccagctg tcgctttttt
     3061 catcagggtg ataaaactgc ccaataatgg cgacaaactg cgtctcttta tccagcggca
     3121 cattcaggga tgccgtgcct tccggtttta cccagacggt gtgttttgcc agtacgttct
     3181 tctccagcgt cttctcttcc tgcataaaca gggtgtcata atccgccttg tcaaaatctt
     3241 cgcgggtttt cagttgccag acatgcacca tcgtggaaag cggcgtatct tccgcatccg
     3301 tattcagggc acttcgggag aagaaatcga gatgcagtat tttgatttgc ttgtagaaaa
     3361 cggctgaggc cacgcttttc gattcatcca ccactttctg aatcagcccg cagccgctca
     3421 gaacaaccgt cagcatcgcc atacagagca gcgccggacg tttaaaacga ataggaaaca
     3481 tgagtcatac tctccctgtt acgaatggaa aaagcgggta ttaatccctg ataacgcccc
     3541 agactgaccg tcactgttcc ggtcgcgggc gcggcaaagg aggcgcgaag gatgccggta
     3601 cggctaatct gtacacgctg ttttcccagc cgggcagcag gcaacaggct gacgggcagc
     3661 gtcagttgca gtcgggcctg acagcgccag cccagataaa cgcgcagcag caccagcaaa
     3721 tcgctgtgca gttgcccgcc gggcagccag ccacgtgctt ctgcggcatc ttcggtatac
     3781 agtttcagca gcaactggct gttgacgtcg gtgccggtac gccccagcag cgttcgcgtt
     3841 ttcaggctga tgggtgaatt ttttgacaga cccgcagggg ccgggagaat tacaggctgc
     3901 ggatcatgag gcaccaccgt cgctttcgtc agcggggcaa gcagtcccac cagtgccgta
     3961 acgccttccg ccgtgcgcgt cggcaggcgc atcacgctga gcagggccag aaaacgggat
     4021 accggcgtgg cgatatggtt ctcgctgccg ggaataccca gcccgatgag tcccagcagg
     4081 caacgggaaa tgtcatccac tgcacctgct tcaaacgacg ccgggtagtt atatttgcgc
     4141 cagatgcggt aatactgggt gataaagcga tggttaaaaa tatcgagaaa cgccatcacc
     4201 gcgtcatgtc cgtcatgacg ctgggtaatg tagtccagat aggcggttgg cagtggcgaa
     4261 tccacgccgt acaggccgag aaaagtggtg cgcactgtgg gtggcgcgtc gggattgtcc
     4321 acgtcacgtt caacgttctt taactcgctg accggaaagc ccatccccgg atgaggacga
     4381 aagcgcaccg catcggcggc ggggctgtcg gtactgccca gcggcgcgtt ctgctggcgc
     4441 tgttccagca actggcaaaa acggtaaaaa ttgatccgcg caatatccgg ggccagccgg
     4501 tcggtcagcc cggtacgcgc tggctgtgat gctctgtcca ttgcaggcac tttcctgtgg
     4561 gttgcagaat taaggttagt tgagtgaata agtggatatc ggcatacagt gcgaagaagc
     4621 ggtgcagcag ttcaccaaac agcgtgatat caccttctcc ggcaaagccg ttactgtcca
     4681 gcgtcacctg aatatccacg ccacgcagca gaaagcctct ttcaaaacgc tgtatcaggc
     4741 tgtgctgaac gtcgacaatc gccgccagac gacggcggtt catctcgctt tcggtccagt
     4801 cgtacagcgc cagcgtgccg cgcagaattt cggcgttatc catcatcgat aaaaagttag
     4861 aaccaaggtg gctgagcacc cgccagtgga agcggtcacg cgccggggga tagcagggct
     4921 gcgtcggggc gcacaggttg cgcacccgca ggacgttctg tgaggcatga actggcgtat
     4981 cgagcagcgt gctctgtaaa gctttacggg gaagctgccc gttagtaccg gtcaggctta
     5041 aggacagggt ttcatcctcc agcatccggt cagtatcaaa cgcatcgccg cccagaatca
     5101 gccaggtgtc atgcaggccg gaagggccgc gctttacccg cgtgtggtaa tagcgttccg
     5161 gcgcatcgtg gcgcagcatc ccgccccggt gacgaaagct ggagaatggc acatacgcct
     5221 gactgccggt atggcgcgaa gaaataatgt tgtcgacgga ataaatctcg gtatgcccgt
     5281 cctgaatgcg catcgggcgc agcagaaatt cgttttccag tggcgaaagg tgtaacggat
     5341 cggcttccag cggaaacagg ttgatgaccg gcacgcagtg cagacggata ttatcgctgt
     5401 caaacggcag gtcgtgaggc cagttctcgt tcagcaacac gtcaatctca aagccggtta
     5461 tcccctccgg ccatgcaacc tgctccagcc cgttcagcgc cacgaacata aacttctcgc
     5521 ggaaggtgaa atactccagc agcaactggt agccgctgaa cgccgattcg cctttcggcc
     5581 acagtcggtc atcgtcatca aaacccatcg gggaaaagtg cgcgtccagc acacggcgtc
     5641 cctgccctgg cagacgcagc cagaactgct gcgtaccaag cgttaaagcc cggtgcagcg
     5701 cacaggccac cgggctgtcg gcactgaggt acagcggcag gcgggataaa tcaatctgtg
     5761 accagtcacc aaccagcggg ccgcattcaa agcgcagacg gataaccgag cgtccgtccg
     5821 gttcatattg caatgacacg tccggcaacg ccagcggatg cagcgtgata tcccgcgtgg
     5881 cgctgtagac gcagcgggtt ctgcgttcgc cgatgggacg ggatagcacc tgaaactctt
     5941 tcagcgtatc actttgcttc atttgttggt gatccgtggt cagttcgacc acggaaagcg
     6001 acggaatagt gcgcaggtag tgcggccaca gcaggctgac cagcccttca gtcagttccg
     6061 gcagatcgtc gtccagcttt tcgcgcaggc ggcccattaa aaaagcgaag ccctcgaaca
     6121 ggcgctccac atagggatcg cgtgcgccgg gtttatcaag attaagcatc gcagcgcggt
     6181 cggggtaagc ctgcgcaaat tctttccccg cttcacgcag gtagcgcatt tcggcctcaa
     6241 agtagcgttg ggtcaggtcg tccatcggag gatttatctt tccttttatt gaaattaata
     6301 ccaacgagta gtgagattag a
//
LOCUS       DAJOTB010000220         6299 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJOTB010000220 DAJOTB010000000
VERSION     DAJOTB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.220"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(271..1464)
                     /locus_tag="OHN28_004538"
     CDS             complement(271..1464)
                     /locus_tag="OHN28_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2299806.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
     gene            complement(1662..4295)
                     /gene="mngB"
                     /locus_tag="OHN28_004539"
     CDS             complement(1662..4295)
                     /gene="mngB"
                     /locus_tag="OHN28_004539"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCQ2299807.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQPGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLQGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIGTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRTFSYRIV"
     gene            complement(4313..6229)
                     /gene="mngA"
                     /locus_tag="OHN28_004540"
     CDS             complement(4313..6229)
                     /gene="mngA"
                     /locus_tag="OHN28_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCQ2299808.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
BASE COUNT         1637 a         1661 c         1356 g         1645 t
ORIGIN      
        1 cccaaaaaaa agcaagcata taaaattaaa aaacatgact ccactttcat ttttatgaaa
       61 tggcatcgtg aagaggcgcc agccaattat aaagatcttg ctcctcaaaa aatgaaacaa
      121 ctcagcctga gataatattt ttgaccgaag ttaacattaa aaatggttaa tagtttgcgc
      181 ctctcacaca ggtccaggca actgcgtaac aggcaaatca aattttcgta aacagtgggc
      241 atcatcagat gcccacaaaa tacagctgat ttacattttc gtctttacac gtgcaatagc
      301 ttcttcataa gaaggcttat ttttttccgt atacgccagt tgataatgct gcaatgcttc
      361 agcattctgg cccgaggttt cataaatttt ggcaatgtta taataggagt tggcgcgcgt
      421 tgtcgccgca tttaccccac tcgctaacga gatcgcttta cgatttgccc aaattgcttc
      481 cgctatgttg ccatttttct gatacgccag acccaggtta ctaaatgcct gtccgtaatt
      541 gccgtctaat tctgttgcct gttggaagag ttgaatggct tgctcaagtt gaccggtttt
      601 ataaacagct tcaccttcgc gattatattt tcttgccagt tgagtatctc cactgttttg
      661 catcgcaggc gaagccggag ctgaagagtc cgtcgcaggc attatcacag tggctaaatc
      721 cctgacgtct aatgtggtgc cgttaaaatt ggcgctggta acagagttga tatcttcaaa
      781 ctcgccgttt ggattgagtt taaacacggt ccagatgttc cctcgttttc ctgcagggac
      841 actataacta cgtaccaggg agctccctac ataaacaaac acttttgcct tactggcaga
      901 gagataatta ctgttgggca gccctttatt gctatagtcc tgcacggcat agatgtaact
      961 ttcaccaaaa tgctttttgc tgatagtgac agtttctggt ccatagctat ccgtatcatc
     1021 aacatccaga ttggcatcag ttccctcttt actatcaaaa tagatatgtc cgccagggaa
     1081 gatcaaatgc gaatcaagat caaaaggctt ttcgccccag ctcaaaacta cacgcatgcc
     1141 atccagattc gtcatcaccg gactgatagc ataagtcatg ccagcgcacg gacatttgac
     1201 caccagattg gaatacccct cttttttgac gattaataga gcatctttat tatcagcaaa
     1261 tgaagtcccc aaactaatgc tgcccgaagt atttgtgata ccagaaacag attgtgcacc
     1321 attgcgttgc agcgtcaccg atgccccttc aatgcgttta tctttcactg tcgcacttaa
     1381 aatatcaatg cttacatcgg cggcataagc acctgcattc atgcccaaca gcgcgagagc
     1441 aactggcaaa acaattttct tcatgtataa atcctttttt taatgaatgt aaggtggaaa
     1501 tttcccagtt attattatcg tgttaaaagg tgaaaaaaaa gaaataagta cacttttcag
     1561 attaacaccc actcaccttg aaacaaaaag gccatccgga gatgacctga actttctgag
     1621 aaacgctgtt ttttcatgtg gtttgcttta ttacacacca ttcagactat tcggtagcta
     1681 aatgtccgcg actgtcccgg taaaaagaga gctaattctt ttaaaacctc atttttttct
     1741 gtattaaagc gttcattcat catggtttct gtacacgtca tcaccttcct gctaaacgcg
     1801 acggtcgcat cacagcttgt caattcagcc ggattaaaca gccgcaatat taattcctgt
     1861 ctgtcttctg ctttttttag cgcgctgatc agacatccca ctggtggcat tttcagcaaa
     1921 ctatagcttt ccggcacatt gaatttggct ttattaagct tcatcgcatc ccacgggatt
     1981 ttgttataac actgaactgg cgtcagccat gcgcgtgcct gctgggctac accggcagtt
     2041 acgggagtac cgatataact aaataaactc agacgacagg aaaatgatcc gcgtaattgt
     2101 gaatcggaca caggcatttt aattcctgaa ggacgccctg gccttaaaag taaatcttct
     2161 tttccctgta atcccacacc acgcaacaac gtaatagcaa aggttttatt ctcttctcca
     2221 atgacttcaa attcacgtaa tccttcgcta aaaacggcta tgccgttacg cccttcctgt
     2281 aagacagcat agttgagcat attccatacc ggaaccggcg cttctttcca gccttcttgc
     2341 tgccagacat tcattgcgca atccttcact ggccgcgtca atgaaccaaa ctgcgtatcc
     2401 gcaagaacaa catctgtgtt aaaaggcgtg ggaatcaata cacgaatacg gtgatcatcg
     2461 gcctggttat caagattaat atccgcatca atgcgtctgc tgttatggct aagagtgatc
     2521 accgtctcca cccctaccct gccagaacat tgctttgcgc gacgctctga cagattacgc
     2581 ggcaccgcta tttcatagcg aattatggcc ctactctgcc aggcttcatg aataatatcg
     2641 tattgcggtt tcgcattcgc tgaggtaatg gcccactctt cttttgctgg cgaataatca
     2701 tattcatccc catcatcgga accttcttca atttgaaaca cccggtcata gcgcacgcca
     2761 ctatctttat caaccagacg aagtgaacca tcctcattga gtgcaatttg ccagaaagca
     2821 ttttccagta ttccctctgt tttacttttt ggcgtgacca cattgccagg ctgattcgct
     2881 tctatataga gcgtgcgata ccccatagaa gggacaattt ggctgatctg aatatcaaac
     2941 tccataaagg gatcataatt gccgtaatga actatttgcc gatcaataag gccgggatcg
     3001 atctcacggg catgtcgaat aaaatatggc acaggctgac cgcgaccgtc tcgcaaatta
     3061 aactggctac cgcgcagtcg aacagttgtg ttgataactt cttcacgcgg ccagggcatc
     3121 aggttaaaca ggacgagttt gtcggcgtcg ctctgcggca tgttgtctgc tattttgcgc
     3181 atatagaacc gaattagatt atccgccatg tcttctgcca gttcgaagcg ggcgacaatc
     3241 tcgcgatgaa ctttatcact gcagcagcag ccgatgctgt catgggcgtg gttttttaaa
     3301 atttctttcc acatcttctc cagcaagccg tggtgataat caaaacctaa agtccaggcc
     3361 agcgttgcca acggttccag cagattaaca atcttatttt caatgcgtgc gtgggcaatt
     3421 ttgatatcca tgcgcgtgga accgatggtg cgatgcacgc gcatatattt gccatcgatg
     3481 aattcacctt tcaggatggc cagactttca cgctgcgctt caatctgctc gaatacctct
     3541 tcaaagcggc tcatcacaaa ctttcgttga ggataaattt cgcgcagttt ctccatcact
     3601 tcgaagatat tttgctgcaa gggcatctgg tcatgaccat tcggcaataa aatttctttg
     3661 gttactgacg ctttttccag cacgtcaaaa taactgtcga ggcgtttacg taatccattt
     3721 tcgtccgcag gtaagtactt accgatggcg tagcccagcg gcagcacctg cgccgtcact
     3781 tcgctaccgt cactgctttg ccacaaaaac tcggttttat cagtaccgtg gcgctcagaa
     3841 catccgcgcc agaacatggt gcgggtaatg ccaaatccat tgtatatatg tggcagttgc
     3901 ccggacatgc caaaggaatc cggtaaataa cctattttca tcggctcgcc aaacgcgagg
     3961 cagtcacgca ttccgtacat cagattacgg actatggatt cagcagaaac aatcgtggta
     4021 tcggtctggg tataccaggg accgataatt aatttcccgc cctcgaccaa cttcttcaca
     4081 cgatctttgt tctccggttt gacagcgaaa taatcttcga ggatcgccgt ttgcccgtcg
     4141 agtacgtaat atttgtattc gttgtcctgt tccagtctgc acaggatctc ttccatatta
     4201 ttgaccagca gaatgcgaga ctcttctgtg gtgaaatacc actcccgatc ccagtgcata
     4261 tgcggggtga tgtgaacgcg agataccgct ttcatcgtcg tttccttttt gtttatggca
     4321 ttacgccatc agtcagatag tttccatgct ttaccgcatg atgccgccag atcaggagaa
     4381 ttgcagtcga gattgcagcc cccaccaacg ccgcaccaaa ccaaccaatt gccgccataa
     4441 caccacccgc gccattatca tgaagtaaaa agagcgagaa aatgccggca ccgggtgtcg
     4501 aaaggccgat attcatcgca ccgacgatag cgcccgttac catagatccc agcacaaacg
     4561 aaccaataac ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg gtgatacctg
     4621 ccaatcctaa cagccaggtg gatttcccgg tttcaatttc aaactcttta aacaggcgcg
     4681 gtgcgagcat cgtggaagcg gttacggtaa atgccgaaac cattttgacg gaggcgaaaa
     4741 tggcatacgg gccgtaaacg ccgttcgcca ttgcccccag acagaatgca taagcggctt
     4801 tgttcactgg cccaccaagg tcaaaggaac acataaaacc gagaatggcg cccaacaaca
     4861 gtgcgttatt tcctgacaga ccgttcagcc aggcggtaag cgagttattg atccatgcga
     4921 caggttcccc tactacaaac agcatcagac tacccgcccc caacgtaccg agcaccgggt
     4981 agagatagaa agtcaggaat ccgttgaatt tactgctaag acgtagatga tttttcaccc
     5041 agcgcatcag ataaccggct atcaatccgc caacgaccgc gccgagaaac ccggaaccaa
     5101 tcatgttggc ggcaagccct gccgcaaagc ctggcgctaa cgccggttta tcagccagtg
     5161 aataggcggt ataggctgcg agcaccggta ccatcaaaat cccgagcatc ccgccgccca
     5221 gcttgcggta catccacaac caggagtttt cttcattaaa caaatcttgt agcccgaaga
     5281 tttgcgacag taataccgcg accgccagca ccgtgccacc cgcgacaatc agcggcacgg
     5341 cgaaagagat cccgctcaac agtgcctgct tcagctccgt tttgacactt ttcaccggtt
     5401 gcgtatcttg ctgtacggta cgcgtctcat cgctacgctt gagggtaagc gcttgttgaa
     5461 tcaacgcttc tgcatggcga atcggctcgg caacaggcac agaaagcgcg ggaatgccgt
     5521 taaaacgctc gctctccttg atagcgactt ccgccgcaaa aatacaggcg gttgcactat
     5581 tgagttgctc cgccgttaaa cgctcttcaa tgccgttagc accttgcttt tcaacgtaga
     5641 tattcacgcc gagtttgcgt ccggcttttt ccagatattc cgcagccatg taggtgtgag
     5701 caatacccgc cgggcaggcc gttacgcaga cgatagttgg cgcataagaa aaagaggcag
     5761 tatgttgcgc gtcccccttg tcatccagcg ccgagagcaa ttcatcgggc gtcgttgccg
     5821 actgaatacg cgccctgagc tcatcatccg ccaggcgcgt ggtcagcgcg gtaagcagtt
     5881 tcatatgcgt agtacctgct tcattgggag gaatggccag caataccact aaatcaaccg
     5941 cttccgggcc atccacgcct tcccactgaa gcggctcgct gagtgtggcg accgcaaacg
     6001 ccgcttcttt taccgcagca gttttgccat gcggcacggc cagcccttca cctaaggcgg
     6061 tcgggccgag gctttcacgg caatacactt cttccagaaa ttgctcagta ctggaaattt
     6121 tccccagagc agcaagacgt tgagtcaacg cgtggatggc ctcttcacgg ctggtaaagc
     6181 gcacattcag acacagcgca tcgcggtggg tcagagtcgt caggttcatc atggttctca
     6241 cttgatcatt tttatagtcg cgcaagtgtg cctgataaaa caatacattt acgtgatca
//
LOCUS       DAJOTB010000221         6295 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJOTB010000221 DAJOTB010000000
VERSION     DAJOTB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.221"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            26..1069
                     /locus_tag="OHN28_004541"
     CDS             26..1069
                     /locus_tag="OHN28_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCQ2299809.1"
                     /translation="MQGIKIYTILASSLISGSVFAQTEISTSNSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            complement(1212..2576)
                     /locus_tag="OHN28_004542"
     CDS             complement(1212..2576)
                     /locus_tag="OHN28_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCQ2299810.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2665..3441)
                     /locus_tag="OHN28_004543"
     CDS             complement(2665..3441)
                     /locus_tag="OHN28_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCQ2299811.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            complement(3446..4084)
                     /locus_tag="OHN28_004544"
     CDS             complement(3446..4084)
                     /locus_tag="OHN28_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCQ2299812.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(4081..5343)
                     /locus_tag="OHN28_004545"
     CDS             complement(4081..5343)
                     /locus_tag="OHN28_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HCQ2299813.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5340..6248)
                     /locus_tag="OHN28_004546"
     CDS             complement(5340..6248)
                     /locus_tag="OHN28_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2299814.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
BASE COUNT         1637 a         1613 c         1531 g         1514 t
ORIGIN      
        1 acaacacatt aataaaggac tacgtatgca aggcattaaa atatatacaa ttctagcctc
       61 ttctcttata tctggtagtg tttttgctca aacggaaatt tcgacttcaa attcgtctac
      121 agttaatgca agttatgttg aaccttcagc ggaaaaaata atatcgccaa gcgataaact
      181 caataacctt tttgaaagaa atatgtctca gccttatatt ttacaaaaaa ttggcgagag
      241 aacttattat gttcaacgct atttttattc cactacgttt tatgtcggag acaaaggggt
      301 tttgctgttt gatgctccgg agggtcgcgg taaatattta ttacaggcaa ttcgtgatgt
      361 gactccatta cccgtaacag cgctggttta ttctcattac catgtggatc acattggcga
      421 tagtccattt tggaacgatg aggcgaagaa agaaggcgtg aatctgcgta ttattgccag
      481 taaagccact gctgaaaaaa tgcaatttat gaacagtcgc ttacccgtcg ccactcaggt
      541 tttaagtaaa aaagatgacc agtttaaatt tgaaaagcag accattgaat tacaccgatt
      601 tgttaaagca ggacataccg acgaccattc tgtatggttg ctaaaacagg aaaaagtagc
      661 tcacagcccg gatctcctga atcctgacca actgccgatg atgggcttcg ccgtatcgga
      721 tacgttggtt taccatgaca gtaatttgcg tcaggttgaa atgcttgact ggaaatattt
      781 tattggcggg catggtaata ttggttcaca cgatgatttt aaatttcaac gccagtttct
      841 taatgatctt cgggatacga caattaaagt cagaaaagaa gaaagttttg gtaaatttat
      901 gaataaaacg gcaaataatc atgctgattt tgcgcgagcc cagcgcgaag caattattaa
      961 aaaagtaact gaagtattac gtccgaaata tggccatatg tatggctacg acgcatctat
     1021 gcctgctaat atcgaaatgg caataaggtt ggtcggttct tattactaag ataaaatcaa
     1081 cttgcaagag actgtaatta tattgcaaag tgaaaattaa cctcggtagc gagatattac
     1141 cgaggttaat attggcatac attacaccct tatcaggcac ttaaacgata catcatatct
     1201 tatctggatt attagatgac gagccatacg caccaggtaa ttaagaatcc ggtaaaaccg
     1261 agaatggtcg ttaacactgt ccaggttttc agaccgtctg ctaccgacaa ccccagatat
     1321 ttggtcacaa tccagaaccc tgagtcatta atatgtgacg cgccaagccc accaaagcag
     1381 gctgccagcg tcaccaatac gcactgaatt ggattcaatc ccatcaccgc ttctgagagt
     1441 aacccgccgg ttgtcaggat tgctacggtt gctgacccct gcgatgcacg cagcgccagt
     1501 gaaataataa atgcggctgg taacagaggc aggtcaatca tttgtaacat gttggcaagg
     1561 gctttaccga cgcccgattc caccagcact ttgccaaata cccctccagc accagtaacc
     1621 aaaatcacta ccgccgcagt gggaagggca gagcccataa tgtcgctggt gtgttgtaag
     1681 ctccagccgc gacgtaaagc caataaccag aatgccagca ccagcgcaat cattagagct
     1741 accattggtg agccgatcaa ctgtagcgta ccaagcaggg gatgcgaagg cggcatcagt
     1801 gttgcagaaa ccgttcccgc catgataatc gcgataggaa taacaattag cgaggtgact
     1861 aacgcgacgc ccggtggatt tattttatcg cttaattttg tcgcgccttc ctcactggcc
     1921 ggagccagtt gcatctgttc cagtacctcg accgacatcg catattggcg cttattgatt
     1981 attttcgctg caaagtagcc aacaatccct acgggaatag aaatcgcaat accgatgatg
     2041 gttagccagc cgatgtctgc gtggagtaat cccgctgcgg cgacagggcc tggatgcggc
     2101 ggtaccgcca cgtgaacagt gagcatgatc ccagcgacag gcaggccaaa tttgagtggt
     2161 gatattttgg caaccttggc aaaaccgtaa atgattggcg caagaataat aaagccgaca
     2221 tcaaagaaga cgggaatgcc gaggaagaac gctgccagag tcagcgcagc gatagttcgt
     2281 ttgtcaccta acttgcgact gaaataatta gccagtgact ctgcaccacc agagtgttcg
     2341 atcatacgcc ccagcatagc gccaagacca ataataatag tgacggaacc aagcacaccg
     2401 cccatcccgg cgatcatcac tttacccact tcgcccgccg gtatacccgc cgcaagtgcg
     2461 actaacaggc tgacgaggag cagagcaacg aatggttgta cctttgcctt gatgaccagt
     2521 agcaacagca tgattacgcc agctaacgca atgcataaca atgtaattgt ggacatggga
     2581 aaccctgtct gaaagttata gttaacctac cccatccgta gatgggggga tgtatgggta
     2641 cgttgtaatt agggattgag cgaattagcg ccaggcgtca aaccagccaa gcccttcttt
     2701 ggtgaggcca cgaggtttgt attcacaacc gatccagccc tgatatccca cttcatcgaa
     2761 caggcggaac agccacggat agttgatttc tccatcgtcc ggttcatgtc gatcaggtag
     2821 tccggcaatt tgtacgtgcg catatttccc ggcgtagttg cggattaaat gtgtcaggtt
     2881 gccatctact ttttgcgcat ggaaagtatc tagttgaata aacacgttat ctcgcgcaac
     2941 ctcttcaaca atagccagtg cctgatactg gctggagaag agataatgag gcttaacgcc
     3001 ggggctgagt gcttcaacca atattcgctt gccgtgtggc gcaaagcggt cggcagcgta
     3061 gcggagatta tcaataaata ctgcccggta ccgttcagca tcttcgccag cgggcacgac
     3121 gcctgccatc acatggactt gttcacaatt gagcgccaat gcatattcca gtgccaggtc
     3181 gatgtctgcg cgtgcttcgt gctcacgtcc gggaagggcg gataatcccc attctcccgc
     3241 attaatatct ccgggagcgg tattgaacag cgccagtgtc agatggtttt gcaccagttg
     3301 ctgctggatt tgctgggggg agtagtcata gggaaacaga aactccacag catcgaaccc
     3361 ggcttttcgc gctgcggcga agcgttcaat aaaaggcact tcggtgaaca tcatggataa
     3421 attagctgca aaacgaggca ttgcattaac tccttaattc cgcaatttca cctgcggtca
     3481 gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc tccagctctt
     3541 ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca tgatttgcca
     3601 gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg cgtttatcgc
     3661 ccggtcggta atagggcacc agcgggacgt ttcccatccg catcaccacg tatggtgtga
     3721 acggacgaat aacgttattg ctgtccagcc cttccaggca ggaaagcgcc gtcgaccacg
     3781 tactgtgcaa atgcaccacc gctttacaga gcggattgtt gcgatacagc gccagatgaa
     3841 agagcacctc tttcgagggg ttgtcaccac ttaaccattc gccatccgcg gtgactttgg
     3901 aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc agtaaattcc
     3961 cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatatccg cgctgaaaga
     4021 atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt gcgaaatcgc
     4081 tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga agttgccaga
     4141 tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc cgggggaaat
     4201 ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc cggaggtctc
     4261 accgcctgcg acaataaagc gtgtcacgcc ttccgctgct aaccgcgccg ctagttgaga
     4321 aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt gaattgctgc
     4381 caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt cctggcccag
     4441 aacccactct gccagctcgt gtgcataagc ggccagagtt tcggttgaga ggcagcgtgc
     4501 cacatcaacc tcacgggctg gtgcaatttg acggtaatgt gccacctggc ggttggtcat
     4561 ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc ctgcttcgcg
     4621 agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca gaccagaacc
     4681 gcccgttacc agtggggcat cgcgcaaggc ttccccctga atttccagat ggtgttcggt
     4741 cagcgcatca agcaccgcgt agcgatagcc ctcttgctgt aagcgagcca gctcctgacg
     4801 aacggctttc acaccttgtt cgaaaacatg tgctgaaacg acgccgcagc gccctgtgga
     4861 ttgcgcttca accagacggg gaagatagct gtcggtcatg ggatttaccg ggtgatggcg
     4921 cattccggat tcggccagca gttgattcat tacgaacaaa tacccctgat aaaccgtacg
     4981 tccgttgact ggcagggccg gagagaagac cgtaaacggt gtgtcgagag catccattaa
     5041 tgcatcggta actgggccga tattcccttt cgccgtactg tcgaaagtag agcagtattt
     5101 gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca gcgattgctg
     5161 tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg cgtcgattgc
     5221 ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac cgttttccac
     5281 cagaaaactg gcgatatccg tcgcgccggt aaaatcatca gcgataacgc caatcttgat
     5341 catgatttcg ctcccggtag ggtgatgcca gagaaaatct tgataactgc gctatcgtct
     5401 tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt tgaggccaat
     5461 ggcagcggga agtgcagggc tttggcggta tcggcaacca gaccaagatc cttaacaaaa
     5521 atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat ccggttttcg
     5581 aacatccatg aatttccggc ggcattggtc acgacgtcat acatcacatc cagcgggatc
     5641 cccgcacggg ctgcaagcgc catcgcttcg gctccggcag caatatgtac gcccgccaac
     5701 aactggtgga taatttttac ggtcgaaccc agtcccggtt ctgctccgat gcgataaact
     5761 tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc gctaccggag
     5821 gccatgacag tcatttcacc gttagcggct tttaccgcac caccagaaac cggcgcatcc
     5881 agcatttcca gaccgaatcc agccagagcg gtagcaattt cttgcgcatc agcattggcg
     5941 atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac gcctttttca
     6001 ccaaacagca cctgtttaac ctgggtcgca ttgaccacca gcaccagcag agcgtccagt
     6061 ttttcggcaa acgtcgcggc gttatcagaa accccgcaag caccagcctc tttcaacgta
     6121 gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa gacctgcgcg gacacatgac
     6181 agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa ctccgatccc
     6241 gttttcatct gctctccttg ttaatttaag tgatattttg tttgatattg tgaat
//
LOCUS       DAJOTB010000222         6187 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJOTB010000222 DAJOTB010000000
VERSION     DAJOTB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.222"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..195
                     /locus_tag="OHN28_004547"
     CDS             <1..195
                     /locus_tag="OHN28_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299815.1"
                     /translation="VMNNLTINYGSQTSPELTGISYEDANDLITELILFIEDVNADND
                     MEYPAHLNKIQALLNFAVAN"
     gene            complement(252..842)
                     /gene="cobO"
                     /locus_tag="OHN28_004548"
     CDS             complement(252..842)
                     /gene="cobO"
                     /locus_tag="OHN28_004548"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCQ2299816.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(839..1597)
                     /locus_tag="OHN28_004549"
     CDS             complement(839..1597)
                     /locus_tag="OHN28_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCQ2299817.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1817..2866
                     /gene="sohB"
                     /locus_tag="OHN28_004550"
     CDS             1817..2866
                     /gene="sohB"
                     /locus_tag="OHN28_004550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCQ2299818.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2902..3153)
                     /locus_tag="OHN28_004551"
     CDS             complement(2902..3153)
                     /locus_tag="OHN28_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCQ2299819.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3533..6130
                     /gene="topA"
                     /locus_tag="OHN28_004552"
     CDS             3533..6130
                     /gene="topA"
                     /locus_tag="OHN28_004552"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCQ2299820.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1595 a         1459 c         1641 g         1492 t
ORIGIN      
        1 gtaatgaata atctgacaat taattatggc tcgcaaacgt caccagaact gacgggtatt
       61 agttatgaag atgctaatga tttgatcact gaattaatac tgtttattga agacgtcaat
      121 gcggataatg atatggaata tcccgcacac ttgaataaaa ttcaagctct tcttaatttt
      181 gccgtggcta attaagccat tcagtatcta aaattagcac ctcaagaggc gaacttttga
      241 ggtgctttca cttagtaatc aatccctatc tgcgctttta caccggcatc aaacgcatgt
      301 ttgatggggc gcagctcact taccgtatcc gccagttcaa gaatatcccg atgacagcct
      361 cgacccgtga taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg aatcacgtct
      421 tccagtggca aatagtcata cgctaccata tatgtcagtt catcgagcaa aaccatatcc
      481 agtgaggaat cagccagcat ccgctttgca tgttgccaga cttcgcggca ggcggcggta
      541 tcggactctc ggttttgtgt atcccaggta aagcccgttg ccattacctg aaactcaacg
      601 ccgtgtggct ccagcagatt tcgttcacca ttaggccagg tgcctttaat aaactgcacg
      661 acgcccactt tttttccgtg accaactgcg cgtgttgccg taccaaatgc cgcggtggtt
      721 ttgccttttc cattgccggt aaagacgatg ataataccgc gttcatcctg ggcctgggcc
      781 acacgagcat ctactttttc tttcactcgc tgctgacgct gttggtagcg ttcatcactc
      841 attgggaaat tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc
      901 ggctgtcatc gcccatcagc cagaggtaga gcggcatgat atcagcgggg gttttaagtt
      961 tctgtggatc ttcggtcggg aaggcactgg cccgcattgc ggtgcgcgta ccgcctgggt
     1021 taatgcagtt gacacgcagg cgctgctgat attcatcggc cagtacctgc atcatccctt
     1081 cggtggcaaa tttcgacgct gcatatgcgc cccagtttgc acgcccctga cgtccaacgc
     1141 ttgatgaggt aaagaccagt gaaccggcgt ccgatttgag taataaagga agcagtgcct
     1201 gggtgagcat aaaggtggca ttaacgttga cctgcatgac gtcctgccag acttgcggat
     1261 cttgttcgct cattgggcaa acatcgccga gcaatccggc attatgcaaa acaccatcca
     1321 gacgcggata attaacggca atgcgctgtg ccagttgttg gcaatcttcg gacgtgcagg
     1381 ttagcaaatc gagaataaac cactgtggct gacgtccagt ttcttcgttt atgtggctgg
     1441 ctacctgacg taatttttct tcattacggc ccaaaagaat cactgtcgca ccatagcgtg
     1501 catacgtcat cgctgcttca cgaccaattc catcgctggc tcccgtcacc agaataatgc
     1561 gatcattgag taaatcttgt tttggctggt aatgcatggc tactcctcaa cgacgttgtc
     1621 tgtcgcgctg ccagtgtact ttatgacttt ggggctttat gcctgaaaca gaagggcatt
     1681 tcaatcagcc tggggcgata acaccgcaaa atggatactt tgtcatactt tcgctgcaat
     1741 aacatctctg cgagacggct aaacatgcct gttgtaaact gtgagccaaa gcgttgttta
     1801 accaaggtgg ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt
     1861 taccgttgtg ctagcgattg cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa
     1921 taaacgccag cgtggcgagt tacgagtcaa caatctcagc gaacagtata aggagatgaa
     1981 agaagaactg gccgcggcgc tgatggacac tcatcagcaa aaacagtggc acaaagcgca
     2041 gaagaaaaag cacaagcaag aagcgaaagc cgcaaaagcg aaagctaaac tgggcgaggt
     2101 ggtaactgac agtaaacccc gcgtctgggt gctggatttt aaaggcagca tggacgccca
     2161 tgaagtgaac tctctacgtg aagagataac ggcggtactc gcagcattca aaccgcagga
     2221 tcaggttgtg ctccgtctgg aaagcccagg tggcatggtg catggttacg ggttggcggc
     2281 ttcgcagttg cagcgtctgc gtgataaaaa cattccttta actgttacgg tagacaaagt
     2341 cgctgccagc ggcggttaca tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt
     2401 tgctattgtg ggttccattg gggtggtggc gcaaatgccc aactttaacc gcttcctgaa
     2461 aagcaaagat attgatatcg aactgcacac cgccgggcag tataagcgca cgctgacgtt
     2521 gctgggtgaa aataccgaag aagggcggga gaaattccgc gaagagttga acgaaacgca
     2581 tcagttattt aaagattttg tgaagcgtat gcgtccgtct ctggatattg aacaggtggc
     2641 aacgggtgaa cactggtacg gacaacaggc agtagagaaa ggcctggttg atgaaatcaa
     2701 caccagtgat gaagttattc ttagcctgat ggaaggccgg gaagtggtca atgtacgcta
     2761 tatgcagcgt aaacgactca ttgaccgatt caccggcagc gcggcagaga gcgccgatcg
     2821 attgttgtta cgctggtggc agcgtgggca aaagccgttg atgtaaaaga caaacgcgag
     2881 gataagacct cgcgttttgc tttaatcaac caggtgatat ttttctgaaa gcacatgggc
     2941 caggtgtttg aacatattaa acaccgcggt gcttttggca gttggtaatc cttgttcatc
     3001 taaaaagtag tcgccggtaa ataccagcac gccattacgc tgcgtgacgc cggtggcttc
     3061 aatccctgcg agcgtatcct catgctcacg aatgattttg ttggcctctt tcaacagcgt
     3121 ttcgcggtcg atgggttgag tctctttgtt cattatttac tccttaaaca aggacattag
     3181 tctacgccag gcatggcttg cagacaaata taccacgctg gtggcaagag cgccttactg
     3241 gcaactttgg attttgcatg ctaataaagt tgcgtatcgg attttatcag gtacagtgtg
     3301 acgctttcgt caatctggca atagatttgc ttgacattcg accaaaattc cgtcgtgcta
     3361 tagcgcctgt aggccaagac ctgttaactc agtcacctga attttcgtga gcagagtcac
     3421 gacaaggggt tgatatccgc agagagcgag tccatatcgg taactcgttg ccagtggaag
     3481 gtttatcaac gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga atatgggtaa
     3541 agctcttgtc atcgttgagt ccccggcaaa agccaaaacg atcaacaagt atctgggtag
     3601 tgactacgtg gtgaaatcca gcgtcggtca catccgcgat ttgccgacca gtggctcaac
     3661 tgccaaaaag agtgccgact ctacctccac caagacggct aaaaagccta aaaaggatga
     3721 acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg aggcgcacta
     3781 tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc
     3841 cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg catggcacct
     3901 gcgggaagtg attggtggtg atgatgcgcg ctatagccga gtggtgttta acgaaattac
     3961 taaaaacgcg atccgccagg catttaacaa accgggtgag ctgaatattg atcgtgttaa
     4021 tgcccagcag gcgcgtcgct ttatggaccg cgtggtgggg tatatggttt cgccgcttct
     4081 atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct
     4141 ggtggtcgag cgtgagcgtg aaattaaagc gttcgtaccg gaagagttct gggaagtcga
     4201 tgccagcacg accacgccat ctggtgaagc gttggcgttg caggtgactc atcagaacga
     4261 caaaccgttc cgtccggtca acaaagaaca aactcaggct gcggtaagtc tgctggaaaa
     4321 agcgcgctac agcgtgctgg aacgtgaaga caaaccgaca accagtaaac ctggcgctcc
     4381 ttttattacc tctacgctgc aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa
     4441 aaccatgatg atggcgcagc gtttgtatga agcaggctat atcacttaca tgcgtaccga
     4501 ctccaccaac ctgagtcagg acgcggtaaa tatggttcgc ggttatatca gcgataattt
     4561 tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa actcacagga
     4621 agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg gcggaatcgt tgaaggatat
     4681 ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt cagttcgtag cctgccagat
     4741 gaccccagcg aaatatgact ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa
     4801 agcacgcggt cgtattttgc gctttgatgg ctggacgaaa gtgatgcctg cactgcgtaa
     4861 aggcgatgaa gatcgtatct taccggcagt cgataaaggc gatgctctga cgctcgttga
     4921 actgacacca gcccagcact ttaccaagcc tccagcccgt ttcagtgaag catcgctggt
     4981 caaagagctg gaaaaacgcg gtatcggtcg tccttctacc tatgcgtcga tcatttcgac
     5041 cattcaggat cgtggctatg tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg
     5101 cgaaatcgtc accgatcgcc tggaagagaa tttccgcgag ttaatgaact acgacttcac
     5161 cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt
     5221 actggatcac ttcttctcgg atttcaccca gcagttagat aaagctgaaa aagatccgga
     5281 agagggtggt atgcgcccga accagatggt tctgaccagc attgactgcc cgacttgtgg
     5341 tcgcaaaatg gggattcgca cagcgagcac cggggtattc cttggctgtt ctggctatgc
     5401 gctgccgccg aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa acgaagtgct
     5461 gaacgtgctg gaaggtgaag acgctgaaac caatgcgttg cgcgcaaaac gtcgttgccc
     5521 gaaatgtggc acggcgatgg acagctatct catcgatccg aaacgtaagt tgcatgtctg
     5581 tggtaataac ccaacctgcg acggttacga gatcgaagag ggtgaattcc gcattaaagg
     5641 ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg
     5701 gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt
     5761 acgtaacggc gaagtggcac caccgaaaga agatccggtg ccattaccgg agctgccgtg
     5821 cgaaaaatca gatgcctatt tcgtgttgcg tgacggtgct gccggtgtgt tcctggcggc
     5881 caataccttc ccgaaatcgc gtgaaacgcg tgcgcctctg gtggaggagc tttatcgctt
     5941 ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat gcgccacagc aggatccgga
     6001 aggtaataag accatggttc gctttagccg taaaaccaaa cagcaatatg tctcttcgga
     6061 aaaagacgga aaggcgactg gttggtcagc attttatgtt gatggcaaat gggttgaagg
     6121 gaaaaaataa cctttaattc cgtctggttt ttataaacaa agggtcgcga aagcggccct
     6181 tttttat
//
LOCUS       DAJOTB010000223         6147 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJOTB010000223 DAJOTB010000000
VERSION     DAJOTB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.223"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(353..1312)
                     /gene="nrdF"
                     /locus_tag="OHN28_004553"
     CDS             complement(353..1312)
                     /gene="nrdF"
                     /locus_tag="OHN28_004553"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCQ2299821.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1322..3466)
                     /gene="nrdE"
                     /locus_tag="OHN28_004554"
     CDS             complement(1322..3466)
                     /gene="nrdE"
                     /locus_tag="OHN28_004554"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCQ2299822.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3439..3849)
                     /gene="nrdI"
                     /locus_tag="OHN28_004555"
     CDS             complement(3439..3849)
                     /gene="nrdI"
                     /locus_tag="OHN28_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCQ2299823.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3846..4091)
                     /gene="nrdH"
                     /locus_tag="OHN28_004556"
     CDS             complement(3846..4091)
                     /gene="nrdH"
                     /locus_tag="OHN28_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCQ2299824.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4338..4667)
                     /locus_tag="OHN28_004557"
     CDS             complement(4338..4667)
                     /locus_tag="OHN28_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2299825.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4819..5163
                     /locus_tag="OHN28_004558"
     CDS             4819..5163
                     /locus_tag="OHN28_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCQ2299826.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5200..5649)
                     /gene="alaE"
                     /locus_tag="OHN28_004559"
     CDS             complement(5200..5649)
                     /gene="alaE"
                     /locus_tag="OHN28_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCQ2299827.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1561 a         1480 c         1521 g         1585 t
ORIGIN      
        1 atagaaagat tcctgttatt tgtttatgtc gatacattac ccaatggaaa tagtcacttt
       61 tttctaccct aacatactga gaatctgagg caacccctga tggcaaatgt ggcatgaata
      121 ttgatgtgag caaatcgccg gaatttccca tgatataagg gctgagagca aatcgagaaa
      181 agttgatatt ttttgtcaaa gatagcaaaa aaacgcctga atattgtggt ttcaggcgaa
      241 taatgcaaca gagtatcagt gtagatcacc acaaattatt tgcgtgatat aaagctattt
      301 ggcgggaatt atttccctgc agcgggtagt gatatttttg aaaataaccc cctcagaaat
      361 tccagtcttc atcttctgtt tcgaccgctt ttcccatcac atatgaagag cctgaaccgg
      421 aaaagaaatc gtgattttca tcggcattcg gcgaaagcgc ggcgaggatt gccggattca
      481 cttccgccat ttctgcggga aatagcggtt cgtagcccag attcatcaac gccttattgg
      541 cgttgtaaca gagaaacgct ttcacatcgt cagcccacgg ggtttcggcg tacagctcat
      601 cggtgtattg caactcgttg tcatagagtt ccagcaacaa atcgaaggcg aaactcttca
      661 attcttcacg tcgtgcctgg gagatctttt ccaggttttt ctgatattta tagccgatgt
      721 agtaaccgtg gactgcttca tcccggataa tcagacggat caggtctgcg gtattggtta
      781 gctttccgcg gctggaaaaa tacatcggca gccagaaacc ggaatagaac aaaaaagatt
      841 caagaaacac actggcgatt ttctttttca gcggatcatc accgcgataa tgttgctgaa
      901 taatctgtgc ttttcgctgc aacggcgcgt tttcttcact ccaggcgtag gcggcatcga
      961 catctttggt ctggcacagc gtcgagaaaa ttgaactgta agagcgggca tgaaccgctt
     1021 ccataaagct gatatttgat aataccgcct cttcatgggg ggtgagtgca tcgggcatca
     1081 gcgagggcgc gccgataaca ttttgcagcg tgtcgagcag cgtcaggcca gtaaaaacgc
     1141 gcattgttag ttgttgttct acgacactta atgtctgcca tgcaggaata tcgttcgaca
     1201 gtggcacctt ttctggtagc cagaaattgc tggtcaggcg attccacacc tccagatctt
     1261 tatcgtcaga tatcttgttc cagttgatgg cgctgatacg tgagagtttc atagatattc
     1321 cttaaagtgc acaggagacg cagccttcaa tttcagtgcc ttccagcgcc atctgacgca
     1381 ggcgaatgta atagagcgtt ttgataccct tgcgccaggc gtaaatctgc gctttgttga
     1441 tatcgcgagt ggtggcggta tcggggaaaa acagcgtcag cgacagcccc tgatcgacat
     1501 ggcgagtcgc ttccgcgtag gtgtcgatga tcttttctgc gccgatttcg taagcgtcct
     1561 gatacagcgc cagattttcg ttagtcataa acggggcagg gtagtaaacg cgcccggttt
     1621 tgccctcttt gcgtatctct actttcgcca caatcggatg aatactcgac gtggcatggt
     1681 tgatataaga gatagaaccg gttggcggca ccgcctggag attctggttg tagataccat
     1741 agcgcatcac gtcgtcgcgc agctgcgccc acatctcacg ggtaggtaac gtaataccgc
     1801 tacgggcaaa cagttcgcca actttcgccg ttttcggctg ccagttactt tgcagatatt
     1861 ggctgaaata ttcaccactg gcatagcgtg actgtttgaa cccggcgaag gtttcaccgc
     1921 gttcgcgcgc cagcaacatc gaggtacgca gtgcgtgcca agtgatggta tagaaataga
     1981 gattggtgaa atcgagtgct tccggcgaac cataagcgat gccttctcgc gccagatagc
     2041 cgtgtaaatt catctgcccc agtccgatgg cgtgcgaggc ggcatttccg gcttcgatgg
     2101 acggcacgct gcggatatga ctcatatctg acaccgccgt taaaccgcgt acggcagtct
     2161 ctaccgtgcg ggcaaagtcg ggggaatcca tggtgtgggc aatattcaac gaccctagat
     2221 tgcaggaaat atcatggcct gtacgggcat agtcgagatt ctcgtcatat tctgaggcgc
     2281 tgttaacctg caaaatttct gagcagagat tgctcatatt tatgcgcccg gcgatagggt
     2341 tagcacggtt aaccgtgtct tcatacatga tgtacggata gccagactca aactggatct
     2401 ctgccagtcg ctggaagaaa tcacgagcgt tgaggtattt tttgcgaatg cgttcatcgg
     2461 caaccagttc gtcatagcgt tcgctgatgg cgacatcggc aaacggtttg ccataaaccc
     2521 gctcaacgtc atagggcgaa aacagcgcca tctgcgcatt ctcttttgcc agatggaaag
     2581 tgatatccgg gatcaccacg cccagcgaca gtgttttaat gcggattttt tcgtcggcat
     2641 tttcccgttt tgtatcgaga aaacgcagaa tatcgggatg atgagcatgt aaatagactg
     2701 ccccagcccc ctgacgcgcg ccgagttggt tggcatagga aaatgcgtct tccagcattt
     2761 tcatcaccgg aatcacgcca gaagattgat tttcaatacg tttaattggc gcgcccgctt
     2821 cccgcagatt cgacagcaaa aatgctacgc cgccgccgcg tttcgacagt tgtagtgcgg
     2881 aatttactgc ccggccaatc gactccatat tgtcttcaat acgcagcaga aaacaggaaa
     2941 ccagttcgcc gcgctgctgc ttaccgcagt tgaggaatgt tggcgtggct ggctgaaagc
     3001 gtcctgacag catctcatcg gtgagctgcg tcgccagtgt ttcatcgcct tgtgccagcg
     3061 tcagcgccac catcgtgacg cgatcggcaa aatcttccag ataacgctta ccgtcgaatg
     3121 tcttcaacgt atagctggtg taaaacttcc atgctcctag gaatgtctgg aaacgaaaac
     3181 cgctggcgtg cgcgtgggca aacagcgtaa tgacaaaatc gcgagagtag cgattaagga
     3241 cactttcatc atagtaacct tcgttaacca gccagttcag gcgctgctgc tggctactga
     3301 aggccacact gttcggacgc acatgcgtcg tcataaaggc gtctactgcc tggcgatctt
     3361 tatcgaactg aatgcgacct gcgctatcgt aaaggttaag catcgcattc agagcgtgat
     3421 aatccatcgt ttcctgcgtc aggcgttctg cggttgtcgt tgccaaaatt cagttactcc
     3481 tttacgaacg ttttcgatat cgctttgcgt acccatgagt tcaaagcggt acagccacgg
     3541 cacgccgcat ttccgggcaa tcacatctcc ggcgcggcca tatgcctcac caaagttgcg
     3601 attacccgaa gcaataacgc cgcgcagcaa cgcccggtta tgctcgtcgt ttaaaaagcg
     3661 aatgacctgt cgtggcaccg cgccagccgt accgccgccg ccgtaagagg gcacgatcag
     3721 gatgtaaggc tcgtctacct gaatccgttc ccgctcattg agcgggatgc gcaccgcggg
     3781 cagccctaaa cgttcgataa aacgctgcgt attttcggag ctgctggaaa agtagacgag
     3841 ctggctcatg cactggccgc gtgtggcgct ggatgcagac gattgatcat gtccgggcgg
     3901 aaaccagacc agctaagatc gcctgcaatc acaaccggca actgacgaaa gccctgagca
     3961 cgcagcgctt ctgccgcttc aggaacgcga tcgacattaa tcatttcaaa atcaaagccc
     4021 cggttttcca tcgcccgttt agtggcgtgg cactgaacgc aatcgttacg agtgtaaata
     4081 gtaatgcgca tgattcgtat ttccgtttaa aatgaagata cggcgcgatg atacgcgtcg
     4141 ggttgtctct ctgttgatac agagatacta gatgtagttg aaaaaagatt caaccacaca
     4201 atatatagca aataatggtc agggttaccc tggggcgagc gtgacggggt agggggattt
     4261 ttgtgattca ccaggcaaaa agaaaccccg aagccaggct tcggggtcaa agacgcgtat
     4321 ttattatcat ttttgcacta cgatttgcgc atgcttaaca gtgcgccgat aaaaataccc
     4381 accgctgccg ctgtacccac actacaccag ggtctttcac gaacaaaaga atccgcgcag
     4441 ccaacggcat cgcgcgcggc ttgctggacg cgagtacgac catgcattcg cgccctggtt
     4501 tctttcagta gagcctgcgc tttactacga gcggcttcgg cttccccttt ggcgtcgctg
     4561 ccccaggact tcaatacaga ttcgagactg tcagctaatt gattgacatc attctggata
     4621 tcctgcacgc catcatcaac gtcgttgcgg ttcggtcggt taaacatatg atcccccatt
     4681 agaattcgat gtaattttca gtgtagacca taattttctt aactgaggtt catcgcggct
     4741 ttatggactt ttctgaaagc gttgtgaatg cgcaatcagg taaggtaggc ccgcggttga
     4801 taataacgag gatcaaatat gtatttacga ccagacgagg tggcgcgcgt acttgaaaaa
     4861 gtcggtttta ctgtcgatgt ggtaacccag aaagcgtatg gttaccgccg tggggaaaat
     4921 tatgtctatg ttaatcgcga agcgcgaatg ggacgtaccg ccctggtgat tcatccaaca
     4981 ttaaaagaac gtagttcaac actggctgaa cccgcttccg atattaaaac ttgcgatcat
     5041 taccagcaat ttccgctcta tttagcgggt gaacgtcatg agcattacgg tatcccgcat
     5101 ggctttagtt cgcgtgttgc gcttgaacgt tatttgaatg gtttatttgg cgaagccagt
     5161 taaagacgcg actggcgatg ccagtcgcga aaagaagggt caggctttta cctgctggta
     5221 acggctgact ttaaacagtc ggcggcaata atcgaggaag tagccataaa ccgcccccat
     5281 caacatcgaa acaacgatgt ttgaactgac tgccgccata atctgatgcc agtctgcgcc
     5341 caccactaac aagatcgcca catacaccgg tgactggaac gtcacataag ccaggatatc
     5401 cgccagattt tttatccagc ctgacgggct aactttgcgt gccgcgcgca taaacagatc
     5461 gcggtacata ccgtatggcc atgcaattaa gatgttcacc ggaatcgcta ccaatctgga
     5521 ataaaaagac tgttcgaagc tcattccgga gaggaaaact tcaatacaca tgttcacgac
     5581 agaacagtaa acaaccatcg cgaacgtatc tgcaactgca tgacgcaagc gtgactgcgg
     5641 tgagaacatg ctaatgctcc ttaataaaag cgtaatggga tgttaatgga gatgaaagca
     5701 gtggatctca ttgccatctg catttttgca tagcgtatat cgctggctat gactaatcaa
     5761 ttagtgaaaa atttttattt aatctgtttt tgtagataaa atattccgta acggcgcgtt
     5821 ttttgtacat tgtggatttt tacatgccga tatgatttat ttttaattaa atcaattgga
     5881 tatgtgcttg cctttatttt cactcatgaa ggtttggggg tgaggtaacg ctataagcgt
     5941 agagaattcg gtgtatgtag tgaccgggtc attttcagca ttttttatga cagataatgt
     6001 acattgacag cagaaagcac cagtgataga tgttttcttt tattcggttg aattgcattt
     6061 ttattcactt tggttttgtc aggcttgcgg aattagcgag cagagagagc cgctctgttc
     6121 accaataacc gaaattactc atcaaaa
//
LOCUS       DAJOTB010000224         6146 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJOTB010000224 DAJOTB010000000
VERSION     DAJOTB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.224"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            134..1408
                     /locus_tag="OHN28_004560"
     CDS             134..1408
                     /locus_tag="OHN28_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2299828.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RNGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRYYQSKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPIND"
     gene            1383..3497
                     /locus_tag="OHN28_004561"
     CDS             1383..3497
                     /locus_tag="OHN28_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HCQ2299829.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVCVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMGPISLETNGLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQI
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMN
                     ITRVIIAHRETTLRTVDRVISI"
     gene            complement(3667..3978)
                     /gene="cvaC"
                     /locus_tag="OHN28_004562"
     CDS             complement(3667..3978)
                     /gene="cvaC"
                     /locus_tag="OHN28_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HCQ2299830.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVC
                     L"
     gene            complement(3956..4192)
                     /gene="cvi"
                     /locus_tag="OHN28_004563"
     CDS             complement(3956..4192)
                     /gene="cvi"
                     /locus_tag="OHN28_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HCQ2299831.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            4353..5130
                     /locus_tag="OHN28_004564"
                     /pseudo
     CDS             4353..5130
                     /locus_tag="OHN28_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            5132..5401
                     /locus_tag="OHN28_004565"
     CDS             5132..5401
                     /locus_tag="OHN28_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HCQ2299832.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            5398..>6146
                     /locus_tag="OHN28_004566"
     CDS             5398..>6146
                     /locus_tag="OHN28_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCQ2299833.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESIRLNTEVV
                     SLRKDAGHRVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYR"
BASE COUNT         1770 a         1268 c         1382 g         1726 t
ORIGIN      
        1 tatttatcct gataactctc ctatgttgta tgtttatatg attttccttg aaacatataa
       61 tgcaaatttt cgatttattt tccatcatta atccagataa acaacaaact aatagtatgc
      121 aaggagacat tatttgtttc gccaggatgc tttagaaaac agaaaaatga agtggcaggg
      181 acgggcaata ttacttcccg gaataccact gtggttaatc atgctgggaa gcattgtgtt
      241 tattacggca tttctgatgt tcattattgt tggtacctat agccgccgtg ttaatgtcag
      301 tggtgaggtc acaacctggc caagagctgt caatatatat tcaggtgtac agggatttgt
      361 tgtcaggcag tttgttcatg aagggcagtt gataaaaaaa ggggatcctg tttatctgat
      421 tgacatcagt aaaagtacac gcaatggtat tgtcactgat aatcatcgcc gggatataga
      481 aaaccagctg gttcgtgtgg acaacattat ttcccgtctg gaagaaagta aaaaaataac
      541 gctagatacc ctggaaaaac aacgtctgca atacacagat gcgttccgtc gctcatcaga
      601 cattatacag cgtgcagagg aagggataaa aataatgaaa aataatatgg agaattacag
      661 atactatcag tcaaaaggac tgattaataa agatcaatta actaaccaag ttgcattata
      721 ttatcaacaa caaaacaacc ttctcagtct gagcggacaa aatgaacaaa atgccctgca
      781 gataaccact ctggagagtc agattcagac tcaggcagca gattttgata atcgtatcta
      841 tcagatggaa ctgcaacgac tcgaattgca gaaagaactg gttaacactg atgtggaagg
      901 cgaaatcatt atccgggcgt tgtctgacgg gaaagttgac tccctgagtg tcactgtagg
      961 gcaaatggtc aataccggag acagccttct gcaggttatt cctgagaaca ttgaaaacta
     1021 ttatcttatt ctctgggtcc cgaatgatgc tgttccttat atttcggctg gtgacaaagt
     1081 gaatattcgt tatgaagcct tcccctcaga aaaatttggg cagttctctg ctacggttaa
     1141 aactatatcc aggactcctg cgtcaacaca ggaaatgttg acctataagg gagcacctca
     1201 aaatacgccg ggtgcctctg ttccctggta taaagtcatt gcgacgcctg aaaagcagat
     1261 aatcaggtat gacgaaaaat acctccctct ggaaaatgga atgaaagccg aaagtacact
     1321 atttctggaa aaaaggcgta tttaccagtg gatgctttct cctttctatg acatgaaaca
     1381 cagtgcaaca ggaccgatca atgactaaca ggaatttcag acaaattata aatctgcttg
     1441 atttgcgctg gcaacgtcgt gttccggtta ttcatcagac ggagaccgct gaatgtggac
     1501 tggcctgcct agcaatgata tgcggtcatt ttggtaagaa tattgacctg atatatcttc
     1561 gccggaagtt taatctctct gcccgtggag caacccttgc aggaatcaat ggaatagcgg
     1621 agcaactggg gatggccacc cgggctcttt cactggagtt ggatgaactt cgagtcctca
     1681 aaacgccgtg tattctccac tgggatttca gtcacttcgt cgttctggtc agcgtaaagc
     1741 gtaaccgtta tgtactgcat gatccggcca ggggcataag atatatcagc cgggaggaaa
     1801 tgagccgata ttttacaggc gttgcacttg aggtctggcc cggaagtgaa ttccagtcgg
     1861 aaaccctgca gacccgcata agtcttcgtt cactgattaa cagtatttac ggtattaaaa
     1921 gaacgctggc gaaaattttc tgtctgtcag ttgtaattga agcaatcaat ctgctaatgc
     1981 cggtggggac acagctggtt atggatcatg ctattcctgc gggggacaga gggctactga
     2041 cgctaatttc tgctgctctt atgtttttta tattactcaa agctgcaacg agtacgctgc
     2101 gcgcatggtc ttcactggtt atgagcacgc tcatcaatgt acagtggcag tcggggctgt
     2161 tcgatcatct tctcagacta ccgctggcgt tttttgaacg ccgaaaatta ggtgatatcc
     2221 agtcacgttt tgactccctt gacacattga gggccacatt taccaccagt gtgatcgggt
     2281 tcataatgga cagcattatg gttgtcggtg tttgtgtgat gatgctgtta tacggaggat
     2341 atctcacctg gatagttctc tgctttacca caatttacat ttttattcga ctggtgacat
     2401 acggcaatta ccgacagata tcagaagaat gtcttgtcag ggaggcccgt gccgcctcct
     2461 attttatgga aacattatat ggtattgcca cggtaaaaat ccaggggatg gtcggaattc
     2521 ggggggcaca ctggcttaat atgaaaatag atgcgataaa ttcgggtatt aagctaacca
     2581 ggatggattt gctcttcgga ggaataaata cctttgttac cgcctgtgat cagattgtaa
     2641 ttttatggct gggagcaggc cttgtgatcg ataatcagat gacaatagga atgtttgtag
     2701 cgtttagttc ttttcgtggg cagttttcgg aaagagttgc ctctctgacc agttttcttc
     2761 ttcagctaag aataatgagt ctgcacaatg agcgcattgc agatattgca ttacatgaaa
     2821 aggaggaaaa gaaacctgaa attgaaatcg ttgctgatat ggggccaata tccctggaaa
     2881 ccaatggttt aagctatcgt tatgacagtc agtcagcacc gatattcagt gctctgagtt
     2941 tatctgtagc tccgggggaa agtgtggcta taactggtgc ttccggtgcg ggaaaaacca
     3001 cattaatgaa agtactatgt ggactatttg aacctgatag cgggagggta ctgataaatg
     3061 gtatagatat acgccaaatt ggaataaata attatcaccg gatgatagcc tgtgttatgc
     3121 aggatgaccg gctattttca ggctcaattc gtgaaaatat ctgtggtttt gcagaggaaa
     3181 tggatgaaga gtggatggta gaatgtgcca gagcaagtca tattcatgat gttataatga
     3241 atatgccaat gggatatgaa acattaatag gtgaacttgg ggaaggtctt tctggcggtc
     3301 aaaaacagcg tatatttatt gcacgagcct tataccggaa accaggaata ttatttatgg
     3361 atgaggcaac cagtgctctt gattcagaga gtgaacattt cgtgaatgtt gccataaaaa
     3421 acatgaatat caccagggta attattgcac acagagaaac aacgttgaga actgttgata
     3481 gagttatttc tatttaaacc atagaggaat tacaagcgta tgaggaatat ttcttcctgt
     3541 tataattcct cgttatgctc agatatctgt tggaggtgga atggaagata gacaatccac
     3601 caagaagaaa tatcattctg tgtggattgt ccaataactg ttctttctta tattaaataa
     3661 tactatttat aaacaaacat cactaagatt atttggactc caattacaca atcttcccgc
     3721 agcatagttc catgcttctg aaggtatccc ttcgggtttt tgcttaattg ttccccctaa
     3781 accggatgga gacattgcag gattaggttt gtgagtggat gcatagtcat atattgcacc
     3841 tccagccaca cccccagcag ctgctccaat tcctcctgca acaaattgcc cggatagtgt
     3901 tcctatagcc atcgcaatat cacgccctga agcaccacca gaaacagaat ctaattcatt
     3961 tagagtcaga gttctcatat gatctccttt ttatcttatc ggatattgaa taataattat
     4021 caccaacaaa gtaacatatt gcagacatta atgcagagaa gcaaaatgta tgcatggata
     4081 aaaagtcctt tcctctaaaa acacaatcat atatagctaa tgcaatatat attgcggtgg
     4141 catttattat aaatgcaaat aacaactcta attttgttct ttttctatcc attacttttt
     4201 atcccattac tttctatccc attaccacac aaacactaac gataatgatt atcgttaaca
     4261 tagtcaagag tgaagggtag gaggccctca accccctata aggggtccgc ttggaaaacg
     4321 gatttcccca cgtcaagaga attgatttga acgagtggct tcgctacaaa cagctcccgt
     4381 ttgtgtgtga aaaacccctc tcaacagatc tcaactctgc acgatatcta caggagattg
     4441 ctccaaaagg cagtatcgtg tcttttaact accgatataa ccccgtcatc gacatgataa
     4501 tgcgtctgag aaatgagaag aaattaggtg aactgcattt cttctcagca gaatttaata
     4561 aaaattctgc actgacccgc catcacctga cctggcgaga ctccgcacaa cagagcaaaa
     4621 gcagcggtgc tctgggcgat ctatcctgtc atctgcttga tttattttgt tttatagggg
     4681 aaagcccggt agtggttcat gggatcaaaa cggtaaaggg gacacgtggg tacaaaatca
     4741 gatggtcagg ttgaagtgga tgataacggc tatgtaatgg gcagttcaga aaagggagct
     4801 tatttcagag ttcatgccag taaatcggaa acagatcata atttgggact gcatatacag
     4861 cttgtatttg aaaatggtga aatcagatat tcaacccacc atgaaaatcg cctgctcctg
     4921 attctattta atgatacgaa tacggaaaca attggcttcg acgctctgaa acgcttgcct
     4981 gatccaccac gggagcttcc attctggtct gattcgttta ttcatcttca tgacgactgg
     5041 tgtgctctta ttaaatatgg tcactcgtct cctaagctgg cggacctgtc atccggcctt
     5101 catatacagg aaataattga ggcattctga tatggatacc attcttttaa ccgggctgtt
     5161 tgctgcattt ttcacaacgt ttgcgttcgc tccccagagc ataaaaacta tcaggacacg
     5221 caataccgaa ggtatctccg ttgttatgta tatcatgttc ctgacaggtg taatctcatg
     5281 gatagcgtat ggcattatgc gatctgattt tgccgtactg atcgccaata ttgtcacact
     5341 gtttctggct gcaccagtgc ttgtaataac acttatcaac cgcaggaaaa aacacgtatg
     5401 aatattgcaa tcatcggcgc gggtcctgcc ggcatcattt ctgcccgtaa tgcgattaaa
     5461 gcaggtcact ccgtggttct gtttgagaag aatacccgaa ttggggggat ctggaacccc
     5521 tggagtggtg gcgcttaccg taatgcctgt atgcagaact ctcgttatac atttcattat
     5581 actggctttc cccctggcga tatcgatgaa tttccaggag tggaacaggt attcagatat
     5641 ctttcggccg tggccggaga ggatgccctc cgtgagtcga tccgactgaa tactgaagtt
     5701 gtttcactca gaaaagacgc cggacaccgg gtgatccgct gcgcttctga aggaaaagac
     5761 acggaagaca tttttgaccg ggtcatcatt gcaacaggtg aactctggca accccgccgg
     5821 ccccccctgc caggtgagga aaacttctcc ggaacgttga tcacgtcgag agattatcag
     5881 gagccagagg catttaaagg aaaaaatatc ctcatcattg gcggcggtgt cagcggtgcg
     5941 gatattgcct cagaccttgt tccctttgcc agaagcgtaa gtctgtccgt caaaaagatg
     6001 ggactttatc tgccgagaca attcccgact ggcccgaatg acatgatgca ctcctatctg
     6061 ggcaggtgtc tgctgagcca aatgaattat gaagatttta tcggttatct cgacaccatg
     6121 atgcctgact acatgcaggc ctaccg
//
LOCUS       DAJOTB010000225         6026 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJOTB010000225 DAJOTB010000000
VERSION     DAJOTB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.225"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..593)
                     /locus_tag="OHN28_004567"
     CDS             complement(<1..593)
                     /locus_tag="OHN28_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCQ2299834.1"
                     /translation="MSTSKYPSEIKINPNKGGKAAIERLVEAYGFTTRQALADHLEVS
                     KSTLANRYLRDTFPADWIIQCALETGTSLKWLTTGQGLKQSSLTAATEELVKFRLAAG
                     KMIEDGSYVFDSSFLPANLSSPIVVQDGLVTYICDQKFSEVLDGNWLINIDGTYSIRK
                     ITRLPKGMIKITTAENSFECAFSDIEVVACIRSTIVS"
     gene            689..910
                     /locus_tag="OHN28_004568"
     CDS             689..910
                     /locus_tag="OHN28_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCQ2299835.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRL
                     RKDKKREKVMINLAALTVDALTDCNVVFN"
     gene            943..1452
                     /locus_tag="OHN28_004569"
     CDS             943..1452
                     /locus_tag="OHN28_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCQ2299836.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTMT
                     GLGASFGLL"
     gene            1460..1756
                     /locus_tag="OHN28_004570"
     CDS             1460..1756
                     /locus_tag="OHN28_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HCQ2299837.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            1874..2215
                     /locus_tag="OHN28_004571"
     CDS             1874..2215
                     /locus_tag="OHN28_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HCQ2299838.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            2283..2516
                     /locus_tag="OHN28_004572"
     CDS             2283..2516
                     /locus_tag="OHN28_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HCQ2299839.1"
                     /translation="MRNIETHTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2516..2743
                     /locus_tag="OHN28_004573"
     CDS             2516..2743
                     /locus_tag="OHN28_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCQ2299840.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKSPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2740..3597
                     /locus_tag="OHN28_004574"
     CDS             2740..3597
                     /locus_tag="OHN28_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HCQ2299841.1"
                     /translation="MSTILKWAGNKTAIMPELKKYLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNCSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVD
                     SSAVYGVRA"
     gene            3594..6008
                     /locus_tag="OHN28_004575"
     CDS             3594..6008
                     /locus_tag="OHN28_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCQ2299842.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQQEKDKSADGDPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
BASE COUNT         1581 a         1396 c         1549 g         1500 t
ORIGIN      
        1 ttcgagacta ttgtacttct tatacaagca accacttcaa tatcagaaaa tgcacattca
       61 aagctattct ctgctgttgt aattttaatc attcctttag gaagccttgt gatttttcga
      121 atggaatagg ttccgtcgat attgattaac cagtttccat caagtacttc agaaaatttt
      181 tgatcacaaa tgtatgtgac gagtccatcc tgaacaacaa ttggtgatga aaggtttgca
      241 ggaagaaatg atgaatcgaa gacatatgaa ccgtcttcaa tcattttgcc tgcggcaagg
      301 cgaaacttaa caagctcttc tgtagctgct gtcagcgagc tttgcttaag gccttgtccg
      361 gtagttaacc atttgaggga ggttcctgtt tcaagagcac attggattat ccagtcagca
      421 ggaaatgtat cccgcaaata cctgttcgct agtgtgcttt ttgatacttc caagtgatca
      481 gctaaagcct gtcgtgtcgt aaagccatag gcttcgacta atcgctcaat cgcagcctta
      541 ccacctttat tgggatttat tttgatctca cttgggtact ttgatgttga catatctctt
      601 ttgcgatcct agtatcagtt ttgtacccaa ttgggtactt gtcacgatta ctacaggctc
      661 accacaagcc aataggagat gttgcatcat gacccctaac atttcaataa ctctgaatac
      721 gccacacgtc acaatcgagc gttatagcga acttactggt ctttcaatcg acacaatcaa
      781 cgatatgttg gcggatggtc gcattcctcg gcatcgtctt cgtaaagaca agaaaagaga
      841 aaaggtaatg atcaaccttg ctgctcttac cgttgatgca cttactgatt gcaatgttgt
      901 attcaactag ttccattttg ggatacatca gaggtgtcga ccatgtttga ttaccaagtt
      961 tccaaacatc cacattttga tgaagcctgt cgtgcattcg cactgcgcca caatctggtg
     1021 caactggcag aacgtgcggg catgaatgtg cagattctgc ggaacaagct gaacccagct
     1081 caacctcatt tattaaccgc accagaaatc tggctgctta ccgatctgac tgaagattca
     1141 acgttggtag atggttttct ggcacagatt cattgtctgc catgtgtacc gattaatgag
     1201 gtagcaaaag agaaactgcc gcattacgtc atgagcgcaa ctgcagagat cgggcgtgtt
     1261 gcagcaggtg cggtatctgg tgatgtaaaa actagtgccg gccgtcgtga tgctatcagc
     1321 agcattaact ctgtaacacg actgatggcg ctggctgctg tttcattgca agcccgtttg
     1381 caggctaatc ctgcgatggc gagtgcagtt gataccatga ctggccttgg tgcttcattc
     1441 ggtttgctgt gaggtgttta tgctgacgaa agaaccatca tttgcatcgc tgctggtaaa
     1501 acaaagcccg gcaatgcact acggtcacgg ctggatcacg ggtgaggatg gaaaacgctg
     1561 gcatccatgt cattcacaag atgaattgct gtctgaattg accacgagga aacggagaaa
     1621 gtccaaatgt atgcggcaga aagtgaagtg gtttatcagt ttcgttacag aggggagagt
     1681 tattcagtac ctgaagatga tttgctctgt tgctatccgt cattgtcggg cgatggcagt
     1741 tactttttca cgttaaagga tgggacgttt ttacggggag agcaggttaa agagacgata
     1801 cgaaaaaatg tatctcctct tgagcgttac cgtaagaaca aagaacgata gctgcgtttg
     1861 ggggatatga agtatggcaa ttaatggcgc tgcagcaact gttccattaa gccccggtga
     1921 acgcctgaat ggacttaatc acattgcgga gttaagggcg aaagtttttg gcctgaatat
     1981 tgagtcagag cttgagcggt ttattaaaga tatgcgtgat ccacgggata tcaataatga
     2041 acaaaataaa cgggcactgg ctgccatatt ctttatggca aaaattccag ctgaacgtca
     2101 tagcatcagc attaatgagc tgaccactga cgaaaagcgg gagctgatta aagcaatgaa
     2161 tcattttcgt gcagtggtga gcttatttcc cagacggcta accatgccga attaaccaac
     2221 taatgaaatt aatggcgtaa acccgccggg catcccttta tctaaattca ggagaattga
     2281 ttatgcgtaa tattgaaacc cacacgacta aaaccggacc ggatgacgca gggcttaata
     2341 ttttactgac agaggctcgt ctggaagaac gccgggcaag ggctgaagca atggctgctc
     2401 gccttgatag cctggcgtgt catatctcat cccgccagct aaaccacgtg gaagcggcag
     2461 aactgctgcg cgtgactgct gaagcaatcc agaacgaagc gcaggagatc cactaatggc
     2521 tgatgcaatg gatctcgtac agcagcgcgt tgaagaagaa cgccagcgcc atatccgtgc
     2581 agcccgtgcc aaatcaccgg gcgtgtcacg cgtactttgc attgaatgtg aagcgccaat
     2641 tccgccagca cgacgccgcg ccattccggg agtgcagctt tgcattacct gtcaggaaat
     2701 cgcagaactg aaaggcaaac attacaacgg aggtgctgta tgagcaccat cctgaaatgg
     2761 gcgggaaata aaaccgccat tatgccagaa ctgaaaaaat accttcctgc tggcccgcga
     2821 ctggttgaac ctttcgcggg ttcctgtgct gtgatgatgg aaacggatta tcccagctat
     2881 ctggttgcgg atattaatcc tgatttaatc aacctctata aaaaggttgc cgctgattgt
     2941 gaatcgttta tatctcgcgc cagagtttta tttgagatcg caaacaggga ggtggcttat
     3001 tacaacataa ggcaggagtt taactgctca actgaaatta ctgatttcat gaaagcggta
     3061 tatttcctgt atctcaatcg tcacggttac cgtggtttat gtcgctataa caagagcggg
     3121 catttcaaca ttccctacgg taattataaa aatccgtatt tccctgaaaa agaacttcgc
     3181 acatttgcag aaaaagccca gcgagcaacg tttatctgcg ccagctttga tgaaacgctg
     3241 gcgatgttga aggcgggaga tgtggtgtat tgcgatccgc catatgacgg tacgttttcc
     3301 ggctatcaca ctgatggttt cactgaagat gaccagtatc acctggcatc cgttcttgaa
     3361 catcggtcat cagaaggaca tccggtcatt gtttctaaca gtgacacatc cctgatccgt
     3421 tcgctgtatc gcaattttac tcaccactat atcaaggtaa aacgcagcat cggtgtggca
     3481 gctggcgagg gtaaatcagc aacagaaatc attgctgttt ccgggccgcg ctgctggatg
     3541 ggatttgatt attcgcgtgg cgtggatagt tctgccgtgt acggagtacg tgcatgagtc
     3601 atgccgatat gaacaactgc tgcggcttta acgaggctgc cgcagcattc tcatggaaca
     3661 gcccgaaaaa ggccattaac ccttatctgg acccggcgga agttgcgccg gtttctacgc
     3721 tttcaaacct gatcactctg tacgctgccg ataacgagca ggaacagttg cgccgcgagg
     3781 cactgagtga tcaggtctgg gagcgttatt tctttaatga atcacgtgat cctgtccagc
     3841 gcgaaatgga gcaggataag ctcattagcc gggcaaagct ggcgcatgag cagcagcgtt
     3901 ttaattcaga catggtcatt ctggcggacg tcaacgccca gccttcccat atcagcaagc
     3961 cgctgatgca acgtattgaa tacttcagca gcctgggcag gccaaaggct tattcccgct
     4021 atttacgtga gacgattaag ccatgtctgg aacgactgga gcatgtacgc gacagtcagc
     4081 tatctgcatc ttttcgcttt atggcaagcc atgaagggct ggacggcctg ctgatcctgc
     4141 ctgaaatgag tcaggatcag gtgaaacgcc tgtccaccct ggtagctgcg catatgagta
     4201 tgtgccttga tgcagcttgt ggtgatttgt atgccaccga tgacgttaag ccagaagaaa
     4261 tccgcaagac atgggaaaag gtggcagcgg aaaccctgcg tctggatgtc atcccacctg
     4321 cgtttgagca actccgtcgg aaaagaaacc gccgtaaacc cgtgccctat gaactcattc
     4381 cgggttcgct ggcgcgtatg ttgtgcgccg attggtggta tcggaaatta tggaagatgc
     4441 gttgcgaatg gcgggaagag cagttgcgcg ctgtttgcct ggtcagcaaa aaagcatctc
     4501 cttatgtcag ctatgaagcc gtgatgcata aacgtgagca gcgccgtaag tcgctggagt
     4561 ttttccgttc tcatgaactg gtgaacgaag acggcgacac gctggacatg gaggatgtgg
     4621 taaacgccag cagcagcaac cctgcgcatc gccgcaatga gatgatggcc tgtgttaaag
     4681 gtctggagct tatcgcggaa atgcgcggtg actgcgccgt tttctacact atcacctgtc
     4741 cgtcgcgttt ccattccacg ctaaataacg gcagaccaaa cccgacctgg acaaacgcga
     4801 cggtaagaca aagcagcgat tatctggtcg gcatgtttgc tgcatttcgt aaggcgatgc
     4861 acaaagccgg gttgcgctgg tatggcgtgc gggtggctga gccgcatcat gatggcacag
     4921 ttcactggca cctgttgtgt ttcatgcgca aaaaagaccg ccgtgccatc actgcattac
     4981 tgcgtaagtt tgccatccgt gaagaccgcg aggagctggg caataacact gggccgcgct
     5041 ttaagtctga gttgattaac ccgcgcaaag gaacgccgac aagctacatc gcgaaatata
     5101 tcagtaagaa cattgacggt cgtggtctgg ctggcgagat cagcaaggaa acgggtaaat
     5161 ctctgcgtga taacgctgaa tacgtgaatg cctgggcgtc tctgcatcgt gttcagcaat
     5221 tccgcttctt tggtattccg gggcgtcagg cttaccgtga acttcgcttg ctggctggtc
     5281 aggcggcaag gcaacagggt gacaaaaaag caggtgcgcc ggtactggat aacccgcgtc
     5341 ttgatgccat tctggctgct gctgatgctg gttgttttgc cacctacatc atgaagcagg
     5401 gcggcgtact ggttccccgt aaatatcacc tcatcagaac cgcttatgaa atcaacgaag
     5461 agccgaccgc ctatggcgat cacggcattc gtatttatgg catctggtca cccattgcag
     5521 agggcaagat ctgcactcat gcagtgaagt ggaaaatggt tcgtaaagcc gttgacgttc
     5581 aggaggcggc agccgaccag ggcgcttgcg ccccttggac tcgtggcaat aactgtcccc
     5641 ttgctgaaaa tttgaaccaa caggagaaag ataaatcagc tgatggggac cccagaacgg
     5701 acattaccag catggatgac aaggagttgc acgattacct gcacagtatg agcaaaaaag
     5761 agcgccggga actggcagca aggttacgcc tggtgaaacc gaaacggcgt aaagactaca
     5821 aacagcgaat tacagaccat cagcgactgc agctcgtgta tgagctgaag tccagaggat
     5881 ttgatggcag cgagaaagag gtcgatttac tccttcgcgg aggcagtatt ccgtcaggag
     5941 caggcctgcg tatcttctat cggaaccagc gtttgcagga agatgatcag tggcggaacc
     6001 tgtattaatc acgcggatta acaatt
//
LOCUS       DAJOTB010000226         6008 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJOTB010000226 DAJOTB010000000
VERSION     DAJOTB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.226"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            145..1266
                     /gene="ompC"
                     /locus_tag="OHN28_004576"
     CDS             145..1266
                     /gene="ompC"
                     /locus_tag="OHN28_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCQ2299843.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1378..2433
                     /gene="apbE"
                     /locus_tag="OHN28_004577"
     CDS             1378..2433
                     /gene="apbE"
                     /locus_tag="OHN28_004577"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCQ2299844.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2507..3571
                     /gene="ada"
                     /locus_tag="OHN28_004578"
     CDS             2507..3571
                     /gene="ada"
                     /locus_tag="OHN28_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCQ2299845.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3571..4221
                     /gene="alkB"
                     /locus_tag="OHN28_004579"
     CDS             3571..4221
                     /gene="alkB"
                     /locus_tag="OHN28_004579"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCQ2299846.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4297..5940
                     /gene="yojI"
                     /locus_tag="OHN28_004580"
     CDS             4297..5940
                     /gene="yojI"
                     /locus_tag="OHN28_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCQ2299847.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1425 a         1508 c         1631 g         1444 t
ORIGIN      
        1 catcttaaaa gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg
       61 gacttgccga ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa
      121 ggcatataac agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct
      181 ctgctggtag caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta
      241 gatctgtacg gtaaagtaga cggcctgcac tatttctctg acaacaagtc agaagacggc
      301 gaccagacct acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccagctgacc
      361 ggttacggcc agtgggaata tcagatccag ggcaacagcg ctgaaaacga aaacaactcc
      421 tggacccgtg tggcattcgc aggtctgaaa ttccaggatg tgggttcttt cgactacggt
      481 cgtaactacg gcgttgttta tgacgtaact tcctggaccg acgtactgcc agaattcggt
      541 ggcgacactt acggttctga caacttcatg cagcagcgtg gtaacggctt cgcgacctat
      601 cgtaacaccg acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggt
      661 aaaaacggta gcgtagatgg cgaaggcatg accaacaacg gtcgtggtgc cctgcgtcag
      721 aacggcgacg gcgttggcgg atctatcact tatgattacg aaggcttcgg tatcggtgct
      781 gctgtttcta gctccaaacg tactgatgat cagaacttcg gcctgaacgg ttatggtgag
      841 cgttatttgg gtaacggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct
      901 aacaacatct acctggctgc tcagtatacc cagacctaca acgcaactcg cgtaggttcc
      961 ttgggttggg caaacaaagc acagaacttc gaagctgttg ctcagtacca gttcgacttc
     1021 ggtctgcgtc cgtctgtagc ctacctgcag tctaaaggta aaaacctggg tgtcatcaat
     1081 ggtcgtaact acgacgacga agatatcctg aaatatgttg atgttggtgc gacctactac
     1141 ttcaacaaaa acatgtccac ctacgttgac tacaaaatca acctgctgga cgacaaccgc
     1201 ttcacccgtg acgctggcat caacactgat gacatcgtag ctctgggtct ggtttaccag
     1261 ttctaatctc gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc
     1321 gtacaaacac agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg
     1381 gaaataagct ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat
     1441 caaaaaccac aacccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaacc
     1501 atgggtacct tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag tgccgaactt
     1561 aaagaaaaga ttcagaccca gctggacgct gacgatcagc tgctttcgac ctataaaaaa
     1621 gattccgcgc tgatgcgctt taacgactcg caaagtttgt cgccgtggcc ggtaagtgaa
     1681 gcgatggccg atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg
     1741 gatataacca tcgggccgct ggtgaatctg tggggctttg gcccggaaca acaaccggtt
     1801 caaattccga gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg
     1861 acggtcatta atcagtcgca tcagcaatat ctgcaaaaag acctgccgga tttatatgtc
     1921 gatctctcca ccgtcggcga aggttatgcg gcggatcacc tggcacgctt gatggagcag
     1981 gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac
     2041 ggtgcaggcc aaccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt
     2101 caggccgtgg tggatatcaa cggtcatggt attagcacct ctggcagcta ccgtaactat
     2161 tacgaactgg acggcaaacg tctttctcat gttatcgatc cgcaaaccgg gcgtcctatc
     2221 gaacacaatc tggtatccgt gacggtgatt gccccgacgg cgctggaggc cgatgcctgg
     2281 gatactggct tgatggtact cggaccggag aaagccaaag aggttgttcg ccgggaagga
     2341 ctggcggtct acatgatcac caaagagggc gataatttta aagcctggat gtcgccgcag
     2401 tttaaaagct tccttatcag cgaaaaaaat taaagcgcaa gattgttggt ttttgtgcga
     2461 tggtgaccgg gcagactaaa ggctatcctt aaccagagag ctgattatga aatacgccat
     2521 acgcttaact gacgatcaac gctggcaatc tgtcttagcc cgcgactcga atgccgacgg
     2581 cgaattcgtt ttcgccgtgc gcaccacggg tatcttttgc cgtccgtctt gccgagccag
     2641 acatgccttg cgggaaaatg tctccttcta cgcaaatgcc agcgaggcgc ttgccgctgg
     2701 ctttcgtccc tgcaaacgtt gtcagccaga caaagccaat cccctgcaac atcgccttga
     2761 taaaattacc cacgcctgcc gacttctgga acaggaaacg cctgtaacgc tggaagcctt
     2821 agccgaccag gtggcgatga gtccatttca tttgcatcgg ttgtttaaag cgactaccgg
     2881 aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aattgctggc
     2941 gaaaggggag agcgtgacta catctattct taacgccgga ttccccgaca gcagcagtta
     3001 ctaccgcaaa gccgacgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga
     3061 aaatctggcg gtgcgttacg cgctggctga ttgtgagctg ggccgttgcc tggtggcaga
     3121 aagtgagcgg gggatttgcg cgatattact gggcgatgat gacgcgacac taatcagcga
     3181 gttacagcag atgtttcccg ctgccgacag cgcgcctgct gatctgacgt ttcagcaaca
     3241 tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga
     3301 cattcgcggc actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg
     3361 tgaaaccgtc agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc
     3421 tgttgccagc gcctgtgccg ccaacaagct agctatcgtt ataccctgtc atcgggtagt
     3481 acgcggtgat ggatcgcttt caggttaccg ctggggcgtg tcgcgtaaag cgcaactgct
     3541 acgccgcgaa gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg
     3601 tggcaagaac cactggcggc tggagcggtg attttgcacc gttttgcttt taacgctgcg
     3661 gagcaactga tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc
     3721 actccaggcg gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg
     3781 acccatcggc aaggttatct ctattcgccc attgatccgc aaacaaataa accgtggccc
     3841 gccatgccgc agagttttca tcatttatgt caacgtgcgg ctacggcagc gggctatcca
     3901 gatttccagc cagatgcctg tttgatcaac cgctacgttc ctggcgcgaa actgtcgctg
     3961 catcaggata aagacgaacc ggatctgcgc gcgccaattg tttctgtttc tctgggctta
     4021 cccgcgattt ttcaatttgg cggcctgaaa cgaaatgatc cgctcaaacg tttgttgttg
     4081 gaacatggcg atgtggtggt atggggtggt gagtcgcggc tgttttatca tggcattcaa
     4141 ccgttgaaag tgggggggca tccactcacc accgactgcc gctacaacct gacattccgt
     4201 caggcaggta aaaaagaata aaaataagaa ttattattgc tgtgcgcacg aagatgttta
     4261 aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg
     4321 cggcagtatc gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta
     4381 ggcattggct taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg
     4441 ctggtattgc cggagtttct ggggttattg ctgctgttga tggcagtcac tctcggatcg
     4501 caactggcgc tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc
     4561 aagcggattc tggataccca tgtcgagcgc attgaacaac ttggtagcgc ctcgttgctg
     4621 gcggggttaa ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct gccggaactg
     4681 gtgcagggga tcattctcac tatcggttca gcggcgtatt tgtggatgct gtcgggcaaa
     4741 atgttgctgg taacggctat ctggatggca gtcaccatct ggggtggttt tatactggtg
     4801 gcgcgggtgt acaaacatat ggcgaccctg cgtgaaaccg aagataagct gtacacggat
     4861 tttcaaaccg tactcgaagg gcgcaaagag ctgactctga accgtgaacg cgccgagtat
     4921 gtgtttaaca acctctacat tcctgatgcg caagagtatc gccatcatat tatccgcgca
     4981 gacaccttcc atcttagtgc cgtgaactgg tcaaacatca tgatgctggg cgcaatcggc
     5041 ctggtgttct ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat
     5101 tcgttgacgt tgttattcct gcgtacgccg ctgctttcgg cggttggcgc gttgccgacg
     5161 ctactgacgg cgcaggtggc gtttaataag ctgaacaaat tcgcgctcgc gcctttcaaa
     5221 gcagagtttc cgcgcccgca ggcgtttccc aactggcaaa cgctggagct gcgtaacgtg
     5281 acgtttagct atcaggataa cgcgttttcc gttggtccaa ttaatctcac cattaaacgt
     5341 ggcgagctgc tgtttctgat tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg
     5401 ctgacgggct tgtaccagcc acaaaatggc gaaatcttgc tggatggcaa acctgtcagc
     5461 ggcgaacaac cggaagatta tcgaaaactg ttttcggcag tgtttaccga tgtctggctg
     5521 tttgatcaac tgctggggcc ggaggggcaa cccgctaacc cgcaactggt tgagaagtgg
     5581 ctggcgcagc tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg
     5641 aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc
     5701 gatattatcc tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt
     5761 tatcaggtgc tgctgccgct gatgcaggag atgggtaaaa ctattttcgc catcagtcat
     5821 gatgatcatt actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc
     5881 gagctgacag gcgaagagcg cgatgctgct tcgcgtgatg ccgttgcccg gacagcataa
     5941 tattacgacg ctgacctggc ggactgctgc cgccaggtca atattctaaa aataccttcg
     6001 tttctaat
//
LOCUS       DAJOTB010000227         5908 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJOTB010000227 DAJOTB010000000
VERSION     DAJOTB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.227"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(86..547)
                     /locus_tag="OHN28_004581"
     CDS             complement(86..547)
                     /locus_tag="OHN28_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCQ2299848.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNV
                     VIEERREDAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEEAPKSRKKTLPKA
                     PPKYQFEENGETKYWSGRGRAPKPIDEALKAGRSLEDFRINKSLNGVTDEQ"
     gene            complement(563..769)
                     /gene="hha"
                     /locus_tag="OHN28_004582"
     CDS             complement(563..769)
                     /gene="hha"
                     /locus_tag="OHN28_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCQ2299849.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAAD
                     HRRAELVMNKLYDKVPSGVWKYVH"
     gene            complement(766..2919)
                     /locus_tag="OHN28_004583"
     CDS             complement(766..2919)
                     /locus_tag="OHN28_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCQ2299850.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPGIAVNPDMTALWSEKQTAIENGDLTVEEFINELYGELTGMISDVDLGEMKIEPAAP
                     AGQFQRLDSPCPSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTD
                     LIKGFKKKSGGTYDTVLVLEDKKTGKLGFPARAKK"
     gene            complement(2924..3499)
                     /locus_tag="OHN28_004584"
     CDS             complement(2924..3499)
                     /locus_tag="OHN28_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCQ2299851.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESVHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLVAIDDTRMDCHSIAYYDIHTLPMISCGH
                     QKIVPFAALIKADECIISKIASYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERR
                     FRQPVSEDNWQQAVSEERAIRCANRLIQCKG"
     gene            complement(3503..4765)
                     /locus_tag="OHN28_004585"
     CDS             complement(3503..4765)
                     /locus_tag="OHN28_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2299852.1"
                     /translation="MGKLSLVEQALLLHKEYYGEGDFGTVVKDYDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSSPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKLGYYDGVYYHGKAR
                     KEEIENAMRFSVHPKFDELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNVAGTYVIFSTNYPKRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPEDV
                     EIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELSVDVIKNARAFLKGTLDLSVQ
                     EADEDIEEREELYKKNGAQPDYLSYLED"
     gene            complement(4886..5227)
                     /locus_tag="OHN28_004586"
     CDS             complement(4886..5227)
                     /locus_tag="OHN28_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299853.1"
                     /translation="MNDCFGCMHPYPAGHWFYSLQDFTPLLENSLFYVFFFFFAGMAG
                     VIISRITNLIKSMRHDNKPSIDNYEQIEIERYMTNEEVGRLVINFMKRTNGEPLLIVF
                     PEYDNGDENRN"
     gene            complement(5238..5726)
                     /locus_tag="OHN28_004587"
     CDS             complement(5238..5726)
                     /locus_tag="OHN28_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCQ2299854.1"
                     /translation="MKLTKYVFFFVTIFSGALCAAEIQGKVIRVLDGDTIEVKTLPAK
                     IVVYEVPIRVRLINIDAPEKKQPFGRWSRNQLKALLAGQSVTVSYTQTDRYGRIVGRV
                     FTTNGTEASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADTNPVPPWEWRH
                     EY"
BASE COUNT         1592 a         1344 c         1145 g         1827 t
ORIGIN      
        1 gcagggataa tttgtttgta ggatgtatta ctcatggatt ttcttcctta acaaaatgat
       61 gatataaaac tatatccttg ccatattact gctcatctgt tactccgttc aaactcttat
      121 tgatacgaaa atcttccaga gaacgcccgg ctttcaacgc ttcatcaatc ggttttggcg
      181 cacgtccacg accagaccag tatttcgttt caccattttc ttcaaattga tacttgggtg
      241 gcgcttttgg taacgttttt ttacgtgatt ttggtgcttc ttcggacaga ccaagcagtt
      301 cttccggtga gaaaccctct ccggcgatta actgaagcag ttcctgacgt ttccgttcac
      361 gctctgctcg ttcagccgct tcacgttcag catcttcacg acgttcttca ataacaacgt
      421 tgagcttttc ctgcatttcg agcaactgct caaaatcaat ctcacgggca aacacacgca
      481 atgaacgaat attcatcata gtccggctga taatgccgta ttcatcttct ttagtcagtt
      541 cgctcataat taatcctctt gtttaatgga cgtacttcca tacgccggaa ggaaccttat
      601 cataaagttt attcatcacc agttctgcac ggcggtgatc tgcggcagaa taaaatgcct
      661 ctgattctga taaaggcagc ttgtaacggt taatctcaat aactttttca agagtatctc
      721 ttgatgtaca ttttcttaac tgaaaaagcc attcctgctt tgttttcact tcttagccct
      781 tgccggaaaa cccagcttcc ctgttttctt atcctcaagg acaagaactg tatcatacgt
      841 tccaccactt ttctttttaa atcccttaat caaatcggtt ttccctgatt taaccagttt
      901 ttcggcctgt gcctgggtga ttttcttacc agaaaactca ctccagattt taaattcaca
      961 tccggtacag aaataacctt tcggcctgat aacaatatgt ttaccacagg aagggcaggg
     1021 agagtccagg cgttgaaact gccctgctgg cgcagcgggt tcaatcttca tctcgcccag
     1081 gtcaacatca gaaatcatgc cagtcagttc accgtacagc tcattaataa actcttcaac
     1141 cgtcaggtcg ccattttcaa tggctgtctg cttttcagac cataatgctg tcatatcagg
     1201 attaaccgct ataccaggca gggcatcaat aagcgcatat ccggtatcag taggaataag
     1261 tttccctttt tccagcgtga tatagtttct tttcttcagt gtttccagaa tggctgcgcg
     1321 ggtagctggc gtaccaatgc caccatgttc atcttttttg tctttatcct tatctttcag
     1381 caatttctta atcgttgggt cagtgacaaa atccgcgacg cgaacaagcg cagcaagcaa
     1441 ggaggcttcg gtaaataatg gcggcggtgt tgttttcttc tcattaacga caacttcttt
     1501 tgtcgtcagt gtttctcctg tgcgaatttt acagagcagt tcaaaagcgg aatcatcatt
     1561 atcttctgat tcaccttcgt ctgtggtttc cgcgccaaga aacgcctcaa atccgctgtc
     1621 agttgttttt ctggcacggg catagaacga ctcatcacca cactgaatgg caaccgatac
     1681 ttcctggtat gctttttcag gcatgaactg aacaagatag tgttgtgcga tcgccaggta
     1741 aacattgcgc tcgtcggtgc tgagtgcgtt aacatcaggc acactggcgg tcgggattat
     1801 tgcagtatgc gcagtcacct ttgcactgtt aaacgctttg cttttacgtg cagaatcaat
     1861 atccagcgac tgaggaaaga ctgatttcag ggcatcgata acctgcggcg cttcgctgaa
     1921 ttgttcatca gaaagatatg agcaatcaga gcggttataa gtaatcgctt tatatttttc
     1981 acgtaattgt tgcgtaatat ccagtgtttt ttgtgccgtc attttgaact ttttgttcat
     2041 gtattgctgg agtctgacca ggttaaacgg caacggtgca gccgtttttt tatcatcagt
     2101 tgctgccgct tcaactgtag ccggttttcc tgcaagggat gctgccgttc cgtctgccca
     2161 cgctttatca agtaatttac ggtctgtcag cggagcaaat tcccctggct tccagttcgc
     2221 tctgataaca tcagcaccac gctgaaagac tccggtcatg gtgtagtaaa aactggattt
     2281 atggttctgg ttagcacggg tacgattcac aatcaggcca agaacgggag tctggacgcg
     2341 cccgacagac agaacaccct gatatcctct ggcttttgcc ggaatggtgt aagcacgcgt
     2401 catggagaag ccatagacgg catcagcaac tgaacgcgcc agcgccttaa ggtaaagccc
     2461 tttgaaatca cgattatctt taagatttgc cagtgccttt ttcactgccg gaagcgtgtt
     2521 gtcgttaatc agaacgcgct ttacgggttt tgtgtttcct gcatattcca ggacttcatc
     2581 aacaagtagc tgtccctcat catcaggatc gccagcgtga acaatttcag tcacgtctcc
     2641 acgtctgata agctcgagaa tcgttttaac ctgttttgct gcactttcaa caggctgata
     2701 cttcacggga taaagacgta aaggaagcgt ttcaaccttc caggctttgt attcaggatt
     2761 atagttttcc ggcggctgtg attcgataat atgaccaaaa cagttagtca caatggcgtt
     2821 atcactttca aaccagccat catggcgggt aaaattgcca ccaagtgcct taacaatatc
     2881 atttgctact gcgggttttt ctgcgataaa aagtctcatt ttgttatcct ttacattgaa
     2941 ttaatctgtt tgcacaacga atagcgcgtt cttctgatac tgcctgttgc cagttatctt
     3001 cacttacagg ttgtctgaat ctgcgctcac agccattaaa ggcaaaaatt ccctcacggt
     3061 taagtatatt cgttgcgata tcctggtctt taattctcaa aaaggctgtt acagcaaaac
     3121 cagaatatga agcaattttt gaaatgatgc attcatcagc ttttattaat gcggcaaaag
     3181 gaactatctt ttgatgtcca catgaaatca tagggagtgt gtgaatatca taataagcta
     3241 ttgaatgaca atccatgcgg gtatcatcaa tagctaccag ccagaatcct gtctggtctt
     3301 ttatttttct gctaatatca gaatctgata caccagggtt taataaatat ccaccatagc
     3361 agacatggaa ggggtgaaca gattcatcaa tatcaggaag ttcttttacc ggattttctt
     3421 tccagtcttt cagaacacca aattcaagag catccagcaa aaactttcgt tcaaatccat
     3481 caaacaatgc atattgcatg tgttaatcct ccaggtaact taaatagtca ggttgcgccc
     3541 cgttcttttt ataaagctct tccctttcct ctatatcttc atctgcttcc tgcacgctca
     3601 gatcaagggt tcctttcaaa aaggcacgag cattttttat tacatccact gataattcat
     3661 ttttggttcc gcgaatcatt ccgattgcga tattaataat ttccttgatt tcagccggag
     3721 tcgttctgtc cagcactgcg ccaagctctt taaggtcaat ttctacatct tctggtaagt
     3781 acattttggc gcaagcggca gcggctttgg ttctgaaagc acctacagag attatcctgt
     3841 caattcgacc tggacgtttt ctaatacgtg ggtcgatacg ttttggatag tttgtagaaa
     3901 agatcacata tgttcctgcg acatttcgcg gtgtatcagc tccatcaaga aaacttaacg
     3961 ttccggcatc agcccgataa agttcatcaa cctcttcagc tataataatg acgggtattt
     4021 tcttctgagc agcagcgtag caaacatctt taaagtaatc gtcggcataa acaaaaacat
     4081 acttatcctg atactttgcc cccaaagctt ttgcgatagt tgtcttgcct gttcctgggg
     4141 ggccagtaaa gagcacttta cgcatacctg actgaccata ccgggtatag aaatcaatat
     4201 cagaaaagaa ctgattaaag tcgttttcca gctcgtcaaa ttttggatga actgagaaac
     4261 gcatagcatt ttcaatctct tcttttcttg cctttccgtg ataataaacc ccatcataat
     4321 acccaagttt ccagatacct ttacgtggta atgagcggtt taatttagcg cgtcctgcct
     4381 cgcaaagatt gtggagcatt gacatacttt tagtagtccc aacggcgtac tccatcctga
     4441 ctttagagtt aagaatgaag cgaataatat gaacaacttc attttgacca agatgaatcc
     4501 tgtatgcacc agaaccatct ttgaacataa gttttttaat tctcccggct ttagccataa
     4561 cagggctact tcccgtatga acaacggttg aagcaacagg ttcgatatcc agattcgaat
     4621 aaattttacg attctttctg tttataaaaa tctctgccac tttaggataa taatcataat
     4681 cctttacaac agtgccaaag tcaccttcac cataatattc tttatggagg agtaacgcct
     4741 gttctacaag agataatttc cccatatgca catcctgtat attaaaacct tgagattact
     4801 tcaggtaacc gaaggtcaga aaaagtcaga ttcgtctgtt cagtttatga ctgatatttc
     4861 aatcttgtta taattattaa ctgtctcaat ttctgttctc gtctccatta tcatattcag
     4921 gaaaaacaat cagcaacggt tcaccattgg tgcgtttcat aaaattgata acgagtctgc
     4981 ctacctcctc atttgtcata tagcgttcta tttctatttg ttcataatta tctattgatg
     5041 gtttattgtc atgtctcatc gacttaatta aattagttat tcttgatata attacacctg
     5101 ccatacccgc gaaaaaaaag aaaaagacgt agaaaagaga attttcaagt agtggtgtaa
     5161 aatcctgtaa agaataaaac cagtgaccag ccggatatgg atgcatacaa ccgaaacaat
     5221 cattcatttt tgtctcctta atactcatgt cgccattccc acggtggaac cggatttgta
     5281 tcagcccata aaccgcgttt ttgttcctgc gcttcccgtt gcaaagctgg taatgattta
     5341 tctgtgttgt aacgttcata tacccatgca gcgccagact gaaccatgaa gcggctggcc
     5401 tccgtgccgt tcgtcgtgaa cacgcgaccg acaatgcggc cgtagcgatc tgtttgcgta
     5461 taagagacgg taacggattg tccggcgagc aaggctttaa gttgatttct tgaccagcgt
     5521 ccaaaaggtt gtttcttttc aggggcatcg atgtttatca atcggactct aatcggaact
     5581 tcatacacaa caatttttgc gggtagtgtt ttaacttcta tggtgtcgcc atcgagaacg
     5641 cgaataactt tcccctggat ttcagccgca cacaacgcac cggaaaaaat ggtgacaaaa
     5701 aagaaaacgt attttgtcaa tttcataact tatgttcacc tttttgtgga tatgtaataa
     5761 gttcatccag aaaacaggat gcaaatgaca agagaaatgc ggatttaaag aaaagccttg
     5821 ctagtgtaat tctctgcttt gtagtagcac tttcccatgt attaataaca tcagtaaaaa
     5881 gttgttcagg cgcaaagtaa aatgctat
//
LOCUS       DAJOTB010000228         5559 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJOTB010000228 DAJOTB010000000
VERSION     DAJOTB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.228"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..652
                     /locus_tag="OHN28_004588"
     CDS             <1..652
                     /locus_tag="OHN28_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299855.1"
                     /translation="GIDGDVAYRDKGNAFTHPNTFKDDVIFEDVLSSKKQININKPGT
                     DLTLFSLAGLSDVSGDYLDFSTRGENNQKVFYRLRGVHYDGLVLLCGGQTYKYWNEAN
                     LKPVKTINGESPDENGNIDISLSPDPSPEIGVVGAVGTYALVAINHAPQILPGSIWAG
                     GDLYYASINNTGSYNTDIATKPFYDTPLIGSWRAMAYSPGGVGDIGTACFLAIRIA"
     gene            652..1041
                     /locus_tag="OHN28_004589"
     CDS             652..1041
                     /locus_tag="OHN28_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299856.1"
                     /translation="MISVDSFRKITNPVFNDEHGLSINIICDIDGIGNDIPFTAMKND
                     CEEHGRVLYERALSGDYGSISKFVKKEQTQKLQTTLRTVGDLSIEAFSIQCAVDAGTA
                     SDAQIENLAELKEAIATRIGENREGIK"
     gene            1038..2540
                     /locus_tag="OHN28_004590"
     CDS             1038..2540
                     /locus_tag="OHN28_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCQ2299857.1"
                     /translation="MSVTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSSYPTC
                     ASLLWGSELADNLVEFSTVMPGADGNNWIVEIVDLGINGNVATPGYSTLPDGSRKLTM
                     ITDGAMTPSKMYDQNQQYKEGLQIGEYINVTFGGDHAGTGTVFALPPTNLSGGKDESF
                     KYNVPTLIAGSQKKAGLLGGSGTLPPAVSEILNQEDAIIVVVRVEEDSDETKMRQNVV
                     NGINALLTSAQINQVTPRILIAPDYSANDYIAEQLEVVTNKLRGVGYIDSPRGATPAD
                     VVNRRQRYGGRMEILRPRVYSTSDVSGLSRPYSAIAAGLRARIDNEKGFWWSKSNQNI
                     YGITGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLSPQWSFECVR
                     RTADVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDEE
                     LNTKESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETV"
     gene            2558..3070
                     /locus_tag="OHN28_004591"
     CDS             2558..3070
                     /locus_tag="OHN28_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCQ2299858.1"
                     /translation="MADSNTYRAFALFVQGERVLNCTEYTPVDMKIIEDDFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMGYLGLIPGAKTRFEIRSAYTDNYGVNFERIDTY
                     EGLITAITDDAQGTDSKSAVGQSVTIAPSYYKRVQNGKIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            3083..3364
                     /locus_tag="OHN28_004592"
     CDS             3083..3364
                     /locus_tag="OHN28_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCQ2299859.1"
                     /translation="MESLLDSMTITLSRPFIIKGESRDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            3473..5113
                     /locus_tag="OHN28_004593"
     CDS             3473..5113
                     /locus_tag="OHN28_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299860.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLEKWTG
                     RAKGAAKWGGRAAAGAVRATGRGIKWGTLGAAGLIGGAAAGALAMNAETSEKLGLAKS
                     YGVGVEKYAAWENIGKAAGLNGENIGDLSEELTNKIGEIGNEKSLNPMLFQIGLTKKR
                     MAGWDREKQFNEVMRRISEMKDEKQAASLADQLMGGEANKIMTYMKATGKSWEQTMSD
                     AQKSNLLTKEGAEGAARAHVSVTNLWGSITSGLADTLGKIGGELAPTFDSVRETFTSW
                     FKDNQGGFVSTITEWVKPENMKKMWDGIVNFGEGCVKFGKIIWAVVKKLEWLIPDEKS
                     DEEQKTYNDEYNRAYQEFMDSGGKYSPNAGLTADNIAKAKAEEAVENMRHPERQEQAK
                     SQAESMLAFVNPLSGMVNKNTAPAALDSSTLNIDTLKQAVSTPAPEQNNKIEINIVGA
                     TDPQSIQQSAASGVLDGLRQAASSYNRGAMFDKPAAAG"
     gene            5149..5538
                     /locus_tag="OHN28_004594"
     CDS             5149..5538
                     /locus_tag="OHN28_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2299861.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRFGKNDALQITGR
                     PNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVKGYWVLRELTET
                     QNSFLMGGTPKSQDFSLTLEYYGASLD"
BASE COUNT         1677 a         1082 c         1439 g         1361 t
ORIGIN      
        1 tggtatagat ggggacgttg catatagaga taagggtaat gcatttactc atccaaatac
       61 ttttaaagat gatgtgattt ttgaagacgt tttatcctct aaaaaacaaa taaatataaa
      121 caaaccgggc actgatttga cgttgttttc acttgctggt ttatcagatg tgtcgggcga
      181 ttatttagat ttttcgaccc gtggagaaaa caatcagaag gttttttata gattacgcgg
      241 agttcactat gatggcctgg tgttactgtg cggcgggcag acgtataaat actggaatga
      301 ggctaatctt aaaccggtta aaacgattaa cggggagtcg ccggatgaaa atggcaacat
      361 agatataagt ctttcaccgg acccatcacc ggaaataggt gttgttggcg cggttggtac
      421 ttatgcgctt gtcgctatta atcatgcacc acaaatactc ccgggctcca tatgggctgg
      481 aggggattta tattacgcta gtataaataa taccggttca tataacacgg atattgccac
      541 aaaaccattt tatgacacac cacttattgg ctcatggcga gccatggcgt attcacccgg
      601 tggagtcggt gatattggga ctgcctgttt tttagctatt aggattgcgt aatgataagc
      661 gttgattcat ttagaaaaat aacaaaccct gtttttaatg acgagcatgg gttatctata
      721 aatattattt gcgatattga cgggattgga aacgacattc cattcaccgc aatgaaaaat
      781 gattgcgagg aacacggtag ggttttatat gaaagagcgc tgagcgggga ttatgggagc
      841 atttctaaat ttgtaaaaaa agagcagacg caaaaactcc agactacatt acggactgtt
      901 ggcgatctgt caattgaggc attctcaatt cagtgtgcag tagatgccgg aaccgcatct
      961 gacgcgcaaa tcgaaaattt ggccgagctt aaagaagcaa tagcgacgcg aattggtgaa
     1021 aatagagaag gaataaaatg agcgtaacta attttttaca cggtattcgc acgctcgaat
     1081 acgacgacgg cacaaaagag atcaacacag tagatatttc cgtgattggg attgttggta
     1141 ctgctcccga ttccagctac ccaacttgtg catcactatt atggggatca gagctcgcag
     1201 acaatcttgt tgagttttcg actgtcatgc caggggccga cggaaacaac tggattgttg
     1261 aaattgttga tttgggaatt aacgggaatg tagcaactcc aggatacagc acactgcccg
     1321 acggttcgag aaaattaaca atgatcacgg acggtgccat gaccccgtca aaaatgtacg
     1381 atcagaacca gcagtataaa gaaggactcc agattggaga atatattaac gtcacattcg
     1441 gtggtgatca cgcaggaacg gggactgtat tcgcgctccc cccaacaaat ttatccggtg
     1501 gtaaagacga atcgttcaaa tataatgttc cgacgttaat tgcaggcagt caaaaaaaag
     1561 ctggattgct cggtgggtct ggaacccttc ctcccgctgt atctgaaata ctaaatcagg
     1621 aggatgccat tattgttgtt gtccgcgtcg aagaggacag cgacgaaaca aaaatgcggc
     1681 agaacgtcgt taacgggatt aacgcattgt tgacgtctgc gcaaataaat caagtaacgc
     1741 cgagaatttt aatcgctcca gattatagtg ctaacgatta tattgcagaa caacttgaag
     1801 tggtgacaaa taaattacga ggcgttggat atatcgattc accgcgaggc gctacgcctg
     1861 ctgacgttgt aaatcgtcgt cagaggtacg gcggcagaat ggaaattctg cgccctcgtg
     1921 tttattctac gagtgatgtt agtggtttat ctcgtccgta ttcagctatc gctgctggtt
     1981 tacgagccag aattgataac gaaaaagggt tttggtggag caaatcgaat caaaatatct
     2041 acggaattac aggactcgaa caagtcgatg attttataat cggtgaaacg aactgcacag
     2101 caaatctgct gaacgccagc caggtcagca caattattcg ctatgatgga ttcagacact
     2161 ggggtaacta tctgtgtagc ctcagtcccc aatggtcctt tgaatgcgtt cgtcggacag
     2221 ctgacgtaat tgaggattca atagcccggg caatgatgac cgattttatt gatcgcccga
     2281 tagacctgca cctcggaaca gacgttgtag agtcgataaa cgcgtatctg cataaattag
     2341 aggagcaggg cgcgattaac ggaggacggg cgtggttaga tgaagagtta aatacaaaag
     2401 aaagtctggc cgctggtaat ctttatatta atgttgactt tggaccgaaa tccccggcac
     2461 aaacaattac gttaatgtac cgcattaata atgattacac agttgaagct ctggcctctc
     2521 tttttaaaga aacagtctaa taatctggag aattgatatg gcagatagta acacctatcg
     2581 tgcatttgcg ttgttcgtcc agggagaacg agttttaaat tgcactgaat acactcccgt
     2641 cgatatgaaa ataatcgaag atgattttaa gacgggcgct atggatacag ctatcactct
     2701 ggacggggga atggagaaaa tgtcagccag ttttaaagtc tctggctccg attctggtgt
     2761 gatggggtat ttgggattaa taccaggtgc taaaacaagg ttcgaaattc gaagtgcata
     2821 tactgacaat tacggtgtca attttgaacg tattgataca tatgaggggc ttattacggc
     2881 gattactgat gacgcgcagg ggactgattc aaaatcagca gttgggcaat cagtaacgat
     2941 tgcgccgagc tattataaac gagttcaaaa tggaaaaatt atttacgaga ttcatcctgc
     3001 aaaaatgaaa cgagtaatta atggtgttga cgttcttgcg ggcgttgctc gtattctcca
     3061 tgtatattaa aaggcaaata aaatggaatc attattagac agtatgacta ttacgctctc
     3121 tcgcccgttt attattaagg gggagagccg cgatacaatt actattcgcg aaccaaaatt
     3181 acgcgaccgc attatgttca gtaacgataa aagtggtctt gaagagcgga cagcgacaat
     3241 gctggcgcgc ctggcaaatc tggagcgtga ggatttatat gcactgcctg cgtgcgatta
     3301 cgatcaaatg gaggccgcgt ttaacgaact ggtaaagcct ccgaaagacc gacatcagat
     3361 atagttattc tgattccgtt tattgcgaaa aaacttgcca tcccgcccga tacgcaaatg
     3421 gacctgccgt accgggtttt taatttctat gtaaacgagg tcatgaaaat cgatggctat
     3481 ttctcaaaac tttaaaacgc aggttgtatt cggtgggcga atagacccgt cgtttcgtcg
     3541 cggtacaaca gaactcaatg atgcgattcg gcagacgtcg tctactgtcg ggaaattaac
     3601 gaaaagtcag gataaattaa aagacaaaat cgcagcaatg aaactggcag gtaaagacgt
     3661 ttctgattta tctgctcagt atcaaaagct ggaccgtcga ataaaagcga caacgcagga
     3721 ccaggaggcg ctgaatactc aacttgctaa aaaacagcgg ctggagaagt ggacgggtcg
     3781 cgcaaaagga gctgcaaaat ggggcggacg agccgccgca ggtgcggtga gggctacggg
     3841 gcggggtatc aaatggggga cacttggcgc ggcagggctc attggcggtg ctgcagctgg
     3901 tgcgctggca atgaatgcag agacgtcaga aaaactcggt ctggcgaagt cctacggcgt
     3961 tggcgtcgaa aaatacgctg cgtgggaaaa tattggtaaa gccgcgggcc tgaacggcga
     4021 aaatatcggg gatttgtctg aagaacttac aaataaaatc ggcgaaatcg gaaacgaaaa
     4081 aagcctgaat cccatgttat ttcaaattgg cctgacgaaa aaacgaatgg ctggctggga
     4141 tcgggaaaaa cagtttaatg aggttatgcg tcgcatctct gaaatgaaag atgagaagca
     4201 agcggcgagc ctcgctgacc agctcatggg gggcgaggct aataagatca tgacgtatat
     4261 gaaggcgacg ggtaaaagct gggaacagac aatgtctgac gcgcaaaagt cgaatttgtt
     4321 aacaaaagag ggtgcggaag gggcggcgcg tgcgcatgta tcagtgacaa atctgtgggg
     4381 ctctattacg tcgggacttg cagatacact cggtaaaatc gggggggaac tcgcgccgac
     4441 gtttgactca gtacgtgaaa catttacatc ctggtttaag gataatcagg gtggttttgt
     4501 cagcacaatt actgagtggg taaaaccaga aaatatgaaa aaaatgtggg atggcatcgt
     4561 caattttgga gaaggttgcg tcaaattcgg gaagattata tgggctgtcg taaagaaact
     4621 ggagtggctt attccggatg aaaaatcaga tgaagaacag aaaacttata atgacgaata
     4681 taatcgggca tatcaagagt ttatggattc gggaggtaaa tattcaccga acgctggatt
     4741 gactgctgat aatattgcga aggcaaaagc cgaagaggct gtcgaaaata tgcggcatcc
     4801 agaacggcaa gaacaggcaa aatctcaggc agaatcaatg ctcgcgtttg ttaatccatt
     4861 atccgggatg gtaaataaaa ataccgcgcc tgcagcactg gattcgtcga cgttaaatat
     4921 tgatacgttg aagcaggcag tctcgacacc tgcgccggag caaaacaata aaatcgaaat
     4981 caatattgtc ggtgcgacag atccgcagtc aatacaacaa tctgcagcgt cgggggtgct
     5041 cgatggactg agacaggctg cgagttcgta caatcgtggc gcgatgtttg ataaaccagc
     5101 tgcggcgggg tgacaatgag tgatgcaata aacggtgctg aggatatcat gctcggcctg
     5161 ggtgatttca tttttgctat atcaacagta gcgtacaaca aactgcaacg tagtgacgcc
     5221 tggcgctggg ctcagcaaac acgattcggg aaaaacgacg cgttgcagat aaccggacga
     5281 ccgaacccca caattactat cgatggaaag ataaacgctt tatttcttga cgggtgcggc
     5341 gtcggcctgt tgacagattt gcgagcgctc gggaacacgg gagaacccca gcagctagtg
     5401 ctggggaccg gggaggtgaa agggtactgg gtgctgcgag aactaactga aacgcaaaac
     5461 agctttttga tgggcggaac acctaaatcg caggactttt cactcacgct cgaatattat
     5521 ggagcttcgc tagattaata caaaatcaag cgctaccag
//
LOCUS       DAJOTB010000229         5543 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJOTB010000229 DAJOTB010000000
VERSION     DAJOTB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.229"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            63..404
                     /locus_tag="OHN28_004595"
     CDS             63..404
                     /locus_tag="OHN28_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299862.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            450..887
                     /locus_tag="OHN28_004596"
     CDS             450..887
                     /locus_tag="OHN28_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCQ2299863.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(929..1924)
                     /gene="wecH"
                     /locus_tag="OHN28_004597"
     CDS             complement(929..1924)
                     /gene="wecH"
                     /locus_tag="OHN28_004597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCQ2299864.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2099..2398
                     /locus_tag="OHN28_004598"
     CDS             2099..2398
                     /locus_tag="OHN28_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCQ2299865.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2493..3404
                     /gene="glyQ"
                     /locus_tag="OHN28_004599"
     CDS             2493..3404
                     /gene="glyQ"
                     /locus_tag="OHN28_004599"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2299866.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3414..5483
                     /gene="glyS"
                     /locus_tag="OHN28_004600"
     CDS             3414..5483
                     /gene="glyS"
                     /locus_tag="OHN28_004600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCQ2299867.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1404 a         1347 c         1427 g         1365 t
ORIGIN      
        1 tttttcagtt ttgtctttcg ttggttactc gtaatgtatc gctggtagat atggagatcg
       61 ttatgaaaac ctcaaagact gtggcaaaac tattatttgt tgtcggggcg ctggtttatc
      121 tggttgggct atggatctca tgcccattgt taagtggaaa aggctatttt cttggcgtgt
      181 taatgacggc aacttttggc aactatgcat atcttcgcgc agaaaaactt gggcaactgg
      241 atgatttttt tatccacgtc tgtcagttgg tagcgttaat cactatcggt ctcttgttta
      301 tcggtgtttt aaacgcacct atcaatgctt atgaaatggt gatctatccc atcgcctttt
      361 ttgtctgctt gtttggtcaa atgcgtttgt ttcgctcggc atgagcaaca taaagttctt
      421 acacattcag gaatgaaagg aatactgtga tggacaacaa aatatcaacc tattctccgg
      481 catttagtat tgtttcatgg gtagccctta ttggcggtat tgttacctat ctgttaggac
      541 tatggaatgc agagatgcag ttaaatgaaa aagggtatta ttttgccgta ctggtattag
      601 gtttgttttc cgcggcgtct tatcaaaaga ccgttcggga caagtatgaa ggcataccta
      661 ccacccccat ttattatatg acttgcctga cggtctttat tatctctgtt gcgttgttgc
      721 tggtaggtct atggaatgca acgttattac tcagcgaaaa aggcttttat gggctggctt
      781 tcttcttaag cttgtttggt gcagtagcgg tgcagaagaa tattcgtgat gccggaataa
      841 acccaccaaa agaaacacag gttacccagg aagaatacag cgaataattc acgtaagccc
      901 ggtcagtcca atgtgaccgg gcttctactt aactcactaa tctgtttctg tcgattcgtt
      961 gtaccagcat agaaagtaat aaactcactg ccagcgtcgc acaaaagatc caaataatat
     1021 ccagtattgg ccagttttta agctcaatcc cccgggtgcg cagtgcatgg ataatcaatg
     1081 catggaaccc gtatatacct aacgaatggc gggagattaa gccaagtccg caaatggtac
     1141 gcgtatacag cgtgttttta accagagtca ataacgcgat tgcgcagata aaaaccatcg
     1201 gcccacagta aagataccag gtatcggcaa aatttccgcg ccactgtaat tcatataatg
     1261 tcccacgaga gataataaaa acccccgtcg caaacagtgc ggcgctcacc cacgacagtg
     1321 ctttatgctg tgtgtccatc atccctatag cgcggcccaa catgccataa aggatgtagt
     1381 aaaaagtatc gccattgata tataagttaa ttggcagcca ttcaaaaccg tcgattttct
     1441 gcggcacggt gtttgggtta gcgatgatac caatcaccac cattagcacc agcaacattt
     1501 ttccgccgac gttcttcacc tgaatcagcg gtgaaaccag ataaatcact gcaatcgcga
     1561 agaaaaacca taaatggtaa aacactggct tttgcagcag gtttttcagc gctaactcca
     1621 cattgatgga ggtaaacagc gcaatgtaga gcagcgcgat tgcgctataa aaaaacaggc
     1681 ataagccgat acgcaagaaa tgacgcgact gggcgctgcg ttcgccaaaa aagagatagc
     1741 cggaaatcat gaaaaatagc ggcacgctga cacgagaggc agaattcaga acattggcga
     1801 tatcccaggt gatggggcta acactatgag cattgatcac ataccaggta gtggtgtgaa
     1861 tcatcaccac cattaaacac gctatccctc gcaggttatc aatccagtaa attttgggct
     1921 gcatctgtgt ctctgtatct ggttaaaaaa agtctgaccg ataaatcatt cagttggcgc
     1981 actggaataa tctgagtttt atcactacag cttatagagg cttaaggaaa ttcgtaagat
     2041 atcagccact ataccgatat aaataataag actcacctgc aaaccagacg gtaatttaat
     2101 gatgatgaac gctttctttc cggcaatggc gcttattgtg ctagtgggtt gttctacacc
     2161 gccacccgtg cagaaagcac aacgggtaaa ggttgatcct ctgcgttcgt tgaatatgga
     2221 agcgttatgc aaggatcagg cggcaaaacg ttataacacc ggcgagcaaa aaatcgacgt
     2281 caccgccttc gaacagttcc agggaagcta tgaaatgcgc ggttatacct tccggaaaga
     2341 gcagtttgtc tgttcttttg acgcggatgg ccattttttg catctttcca tgcgttaagc
     2401 cctgcttttt cccgtttcgt actgtatatc ttccatccag cgggtatact gatcccttcc
     2461 tttaaatcca cacgtatcca gcacgaaata atatgcaaaa gtttgatacc aggaccttcc
     2521 agggcttgat cctgacctta caggattact gggctcgcca gggctgcacc attgttcaac
     2581 cattggacat ggaagtcggc gcgggaacct ctcacccaat gacctgtctg cgcgcgctgg
     2641 ggccagaacc gatggcggct gcttatgttc agccttctcg tcgcccgacc gatggtcgct
     2701 acggcgaaaa ccccaaccgt ttacagcact actatcagtt ccaggtggtc attaagccat
     2761 cgccggacaa tattcaggag ctgtacctcg gttctctgaa agagctgggc atggacccga
     2821 ccatccacga catccgtttc gtggaagata actgggaaaa cccgacgctg ggtgcctggg
     2881 ggctgggctg ggaagtgtgg ctaaacggca tggaagtgac gcagttcact tacttccagc
     2941 aggttggtgg tctggaatgt aaaccggtta ccggtgagat cacctacggt ctggaacgtc
     3001 tggccatgta cattcagggc gtagacagcg tttatgacct ggtctggagc gacggcccgc
     3061 tgggtaaaac cacctacggc gacgtgttcc atcagaacga agtggagcag tccacttaca
     3121 acttcgaata cgcggatgtg gacttcctgt tcacctgctt cgagcagtac gagaaagaag
     3181 cgcagcaact gctggcgctg gaaaatccgc tgccgctgcc agcctacgag cgtattctga
     3241 aagccgccca cagcttcaac ctgctggatg cgcgtaaagc catctccgtc accgagcgtc
     3301 agcgctatat tctgcgcatt cgcaccctga ccaaagcagt ggcagaagca tactacgctt
     3361 cccgtgaagc cctcggcttc ccgatgtgca acaaagataa gtaagaggcg gctatgtctg
     3421 agaaaacttt tctggtggaa atcggcactg aagagctgcc accaaaagca ctacgcagcc
     3481 tggctgagtc ctttgctgcg aactttactg cggagctgga taacgctggc ctcgcacacg
     3541 gcaccgttca atggtttgct gctccgcgtc gtctggcgct gaaagtcgct aacctggcgg
     3601 aggcgcaacc ggatcgtgaa atcgaaaaac gtggtccggc aattgctcag gcgttcgacg
     3661 ctgaaggcaa accgagtaaa gcggcagaag gttgggcgcg tggttgcggt attaccgttg
     3721 accaggctga gcgtctgact accgataaag gcgaatggct gctgtatcgc gcccatgtga
     3781 agggcgaaag caccgaagca ctgctgccga atatggttgc gacttcgctg gcgaaactgc
     3841 cgatcccgaa actgatgcgt tggggcgcaa gcgacgtgca ctttgtgcgt ccggttcaca
     3901 ccgtgaccct gctgctgggc gacaaagtta ttccggctac cattctgggt attcagtccg
     3961 atcgcgtgat tcgcggccac cgctttatgg gcgagccgga attcactatc gacaatgccg
     4021 atcagtatcc agaaattctg cgtgagcgcg ggaaagtcat cgccgattac gaagaacgta
     4081 aggcgaagat taaagccgat gctgaagaag cagcacgtaa gattggcggt aacgctgatt
     4141 taagcgaaag cctgctggaa gaagtggctt cgctggtgga gtggccggtc gttctgaccg
     4201 cgaaattcga agagaaattc ctcgcggtgc cgtctgaagc actggtttac accatgaaag
     4261 gtgaccagaa gtacttcccg gtgtatgcga acgacggcaa actgctgccg aactttatct
     4321 tcgttgccaa catcgaatcg aaagatccgc agcagattat ctccggtaac gagaaagtcg
     4381 ttcgtccacg tttggcggat gccgagttct tcttcaatac cgaccgtaaa aaacgtcttg
     4441 aagataacct gccgcgcctg caaaccgtgt tgttccagca acagttgggg acgctgcgcg
     4501 acaaaactga ccgcattcag gcgctggccg gctggattgc tgaacagatt ggcgctgacg
     4561 ttaaccacgc tacccgtgcg ggcctgctgt ctaagtgtga cctgatgacc aacatggtct
     4621 tcgagttcac cgacacccag ggcgttatgg ggatgcacta cgcgcgtcac gatggcgaag
     4681 cggaagacgt agctgtcgcg ctgaatgagc agtatcagcc gcgctttgcc ggtgatgacc
     4741 tgccgtctaa cccggtagcc tgtgcgctgg cgattgctga caagatggat accctggcgg
     4801 gtatcttcgg tatcggccag catccgaaag gcgacaaaga cccgtttgcg ctgcgtcgtg
     4861 ccgcacttgg cgtgctgcgt attatcgttg agaagaacct caaccttgat ctgcaaacgc
     4921 tgaccgaaga agcagtgcgt ctgtatggcg ataagctgac taatgccaac gtggttgatg
     4981 atgttatcga ctttatgctc ggtcgcttcc gcgcctggta tcaggacgaa ggttacaccg
     5041 ttgacaccat ccaggcggta ctggcgcgtc gtccgactcg tccggctgat ttcgatgccc
     5101 gtatgaaagc ggtatcgcac ttccgtaccc tggaagcagc tgcagcactg gcggcggcga
     5161 acaagcgtgt atctaacatc ctggcgaaat ctgatgaagt gctgagcgat cgcgtgaatg
     5221 cctctactct gaaagagccg gaagaaatta aactggcgat gcaggttgtg gtgctacgtg
     5281 acaagctgga gccgtacttt gcggaaggtc gttaccagga tgcgctggtc gaactggctg
     5341 agctgcgtga accggtcgat gctttcttcg ataaagtgat ggtcatggtt gatgataaag
     5401 aattgcgtat caaccgtctg accatgctgg aaaaactgcg cgaactgttc ctgcgcgttg
     5461 cggatatttc gctgttgcag taataacgcc gttattaaaa agcctgccat ctggcaggct
     5521 ttttttatcg ctaaataata cag
//
LOCUS       DAJOTB010000230         5474 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJOTB010000230 DAJOTB010000000
VERSION     DAJOTB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.230"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="OHN28_004601"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="OHN28_004601"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCQ2299868.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="OHN28_004602"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="OHN28_004602"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCQ2299869.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="OHN28_004603"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="OHN28_004603"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCQ2299870.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="OHN28_004604"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="OHN28_004604"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCQ2299871.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="OHN28_004605"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="OHN28_004605"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCQ2299872.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..5388
                     /gene="citG"
                     /locus_tag="OHN28_004606"
     CDS             4510..5388
                     /gene="citG"
                     /locus_tag="OHN28_004606"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCQ2299873.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1267 a         1542 c         1440 g         1225 t
ORIGIN      
        1 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcaatttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaaa
      601 attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta tctgattcaa
      661 caggctgcgg cacaatgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcaa cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgctac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca
     1021 ccgcccatcg aactagttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcaaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagtatgt tgtttgtgcc tggtgctaat gccgcgatgg tcagcaactc
     1621 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtag cattgcgtga
     1681 aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgcttt atcgcgatat
     1741 tgaaaccatt gtgcgtgtaa acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1921 taccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggcgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gctcgctgct ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc cgatctggaa gaagcgattc gtcgctctgg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgactc tggcctccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctgc
     2821 gcggtccact ggcggaagaa atctcccgtg gtctgctggc ggaaccggtg caaatccact
     2881 ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgat gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctactccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatc
     3121 tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gctacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgttaact
     3241 ctggctactt caaagaaggt ttctccatgc agaccggcac cggcggcgct tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gcggtattac cgcgacgatg gttgacctgc acgaaaaagg tctgatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc caagctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccaatac gctaactggg ggtcgaaagg cgcatcggtt gatcgtctcg
     3541 acgtggtggt actgagtgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgacgctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggcatcg
     3781 ctgttaaccc ggcacgtccg gagctggcag aacgtctgca ggaagcgggg atcaaagtgg
     3841 tttctattga gtggctgcgt gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgcctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgccaggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4201 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgacattc
     4381 tctcccgccg cgattattca ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag
     4441 ctgtttgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcgc
     4501 tgctaaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4561 attgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa cctgtcaccg
     4621 aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc
     4741 tgtagtgcag aaatggcacc ggaagcggta ctcaacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg aagcattttt
     4861 tctttagggc tactgtgtgc ggcaattggc cgtttgcttc agctcaacca gccggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg
     4981 cgtaccaata attcacgact gacggcaggt caacggttgt accagcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcaggttat ccactggtaa tcaaccacgc cttgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgaactgg cactgctcga taccttgctc
     5161 ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacacta ttaaacaatg ggggcatccg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat tcttacctgg tttttagcac agatttaatt atttaagcac
     5401 ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca aaagataata
     5461 tatggaaact attg
//
LOCUS       DAJOTB010000231         5402 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJOTB010000231 DAJOTB010000000
VERSION     DAJOTB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.231"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(125..1408)
                     /gene="aroA"
                     /locus_tag="OHN28_004607"
     CDS             complement(125..1408)
                     /gene="aroA"
                     /locus_tag="OHN28_004607"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCQ2299874.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1479..2567)
                     /gene="serC"
                     /locus_tag="OHN28_004608"
     CDS             complement(1479..2567)
                     /gene="serC"
                     /locus_tag="OHN28_004608"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCQ2299875.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2766..3458)
                     /locus_tag="OHN28_004609"
     CDS             complement(2766..3458)
                     /locus_tag="OHN28_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCQ2299876.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3588..5348
                     /locus_tag="OHN28_004610"
     CDS             3588..5348
                     /locus_tag="OHN28_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCQ2299877.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1401 a         1381 c         1265 g         1355 t
ORIGIN      
        1 aagttacatt gataattaac gctaccatta ataaaaatac cccacaaggt ccgaaaaaaa
       61 acgccggcaa tgtgccgacg tctttgaaaa gaaaggattg tctatgttat cgcccgttat
      121 tcattcagcc gggttgactt atccgcgcca attgctcgaa atagtctgga aaagttttag
      181 cggtacattt tggatcaaga atcgtcacag gcgtatctga taatgcgacc agcgagaaac
      241 acatcgccat ccggtgatca ttataagtgg cgatctcagc aaatttcagt ttttccggtg
      301 gcgtaatgcg aatgaaatca tgcccctctt cgacttccgc accgacttta cgcagttctg
      361 ttgccatcgc aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg
      421 tggtggtgcc ttttgcaaat aacgccgcgg tggcaatggt catcgccgca tcgggaatat
      481 ggttcatatc catatcaatg gcgttcagtt caccacgcgt gcaggaaata taatcatcgc
      541 cccagcaaat ggtcgcgccc attttttcca gaacatcagc aaagcgaata tcaccctgca
      601 tactgttgcg tccaatgccg gtcactttta cagtgccgcc tctgattgct gctgctgcca
      661 ggaaataaga agccgaagat gcatcgcctt caaccaaata agtgcccgga gactggtaag
      721 actgcccccc ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg
      781 tcttcatcag attgagggtg atatcgatat aaggtttaga aaccagatcg cctttaatac
      841 gaatcaccgt atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaactggc
      901 tggaaacgga gccatcaaca tcaacgttgc cgccagtaaa gccgccctgt aaacgcaatg
      961 gcggataatt ttcttgttcc aggtaagtga tcttcgcccc gccctggcgc agcgcatcca
     1021 ccagatgacc aatcgggcgt tctttcattc gcggttcacc gctcagtaca atatcattgc
     1081 tacccaggca aagtgctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca
     1141 actcccgggc actttctgcg tgtaatggac caccgttacc gataatttcg caacgcgtac
     1201 gatcggctga aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca
     1261 cgtcatcgct atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca
     1321 ataaagcgcg gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag
     1381 cgatgggttg taacgttagg gattccatga actcaactct ctacaacaga aataaaaacc
     1441 ccacaggctg gctgtgggga ttatgcaaaa tttcgacatt agccgtgacg acgttcgaac
     1501 tcaaccatga agtctgtcag tgctttaaca ccttccagcg gcatggcgtt ataaatagac
     1561 gcacgcattc cgccgaccac tcggtgacct ttcagcgcat gtaagcccgc agcgaaagac
     1621 tcttcgagga acaatttatc aagcgcgcta tccgccaact ggaacggcac gttcatccgc
     1681 gaacggttag ctttcgccac atcattgcgg tagaaatcgc tgttatcaat caccccatac
     1741 agcagttctg ctttttgctg attgatctta tccattgcag ccacaccgcc gttcgctttc
     1801 agccatttga agaccaggcc agataagtac caggcaaatg tcggcggcgt gttgaacatg
     1861 gaatcgttat cgttgaggat ggagtaatcc agaatcgatg ggcaggcgat attcgctttg
     1921 cccagcaaat cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc
     1981 gcgccagcgt aaattacacc ataacggctg acatcaatcg gacgggaaag aatggttgaa
     2041 gagaagtcag cggcgaccac cacgtcttta ccgaagtctg gtgtttcatc gatagcgata
     2101 ccatcgatgg tttcgttcgg gcagtaatgc atataagcag cattatcaga gagttgccat
     2161 tcactcattg gcttaactgc gcgaagacca tcaacggtca ctttcgcatc aaacacattt
     2221 ggtgtgcagt attttttcgc ttctttgata gcacttgctg cccagtaacc cgcatcaaca
     2281 taatctgcag tggttttatc accaagaata ttcaacggta ctgcagcaaa ttgaccgcga
     2341 ccaccgccat ggcagaacaa tactttgtag ttggagggga cgttaagaag atcgcgaaaa
     2401 tccttctcgg cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc
     2461 accgacgtac caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct
     2521 gccggtagca ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac
     2581 gttgcgttgc tattaacccg cggtcaatcg attgcgacca caggtaatac attattccag
     2641 tggctatcgg ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa
     2701 aaatgtggct tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt
     2761 tgctgtcagg tcacccgttt agctcgactt gccttcgtcc attctggatt tgcacagcgc
     2821 ggacataatt gtttttcccc cgcgttcata tgaatgattt cactgcaacg gatgcaggca
     2881 tagtccgccg actccggcac cactttgtaa cgctgagtac agtcactggg taaaataagt
     2941 aaccccggtt tcaccttgtc atcttcaaag aaataaacac tgatttgccc gttggtttcg
     3001 agaatcgcca gacgtacctg ccccaactgc tctacaccgc gcaatcgtag ctccataaag
     3061 aactcaaatt ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca
     3121 ataatgacaa ccggcttgcc ttccagaaga tcttccagtt tttcactgtg cgccatcaac
     3181 cacattacca ggcggtataa caacgccaga gtaataaaga cgataagtac cgggaccatc
     3241 ggcacatcat cataaaacgc cacatctccc gccgctgaac ccagtgtcag aatgattaac
     3301 acttcaaaca gcgacatctg ccgcacaccg cgtcttccgg tcattttgag gaacaaaaaa
     3361 accagtacaa aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact
     3421 ttatcaaatg ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt
     3481 atccgctaaa agcatagcca acagttcatc ggcatggcgg cggtgatagc gcctttgaga
     3541 taaaacccgt attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat
     3601 ttatccccgg caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt
     3661 cagacctcgg ttttcagatt gaagaggcct cctggctgaa tcccgtgcct aatgtctggt
     3721 ctgtacatat tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca
     3781 aaaaagcggc gctggcttct gccctcggtg aatatttcga gcgtctctca accaactact
     3841 tttttgcgga cttctggctg ggcgaaacca tcgccaacgg cccattcgtg cattatccca
     3901 acgaaaaatg gttcccactg accgaaaatg acgatgtacc agaagggctg ctcgatgacc
     3961 gtctgcgcgc attttacgat ccggagaatg aactgaccgg tagtatgctg attgacctgc
     4021 aatccggtaa cgaagatcgt ggtatttgcg gtctaccgtt tacgcgccag tccgacaatc
     4081 agaccattta tattccgatg aatatcattg gtaacttgta cgtttctaac ggtatgtctg
     4141 ctggcaatac ccgtaacgaa gcacgcgttc aggggttatc cgaagttttc gaacgttacg
     4201 tgaaaaaccg cattattgct gaaagcatca gcttgccgga aatcccggca gacgtgctgg
     4261 cacgttatcc agcagtagtt gaagcgatcg aaacgctgga agcggaaggt ttcccgatct
     4321 tcgcttatga cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc
     4381 ctgctaacgg tacctgcttt gcctcattcg gtgcgcatcc tgattttggc gttgcactgg
     4441 aacgtaccgt gaccgagttg ctgcaaggtc gtggcctgaa agatctggat gtgtttactc
     4501 caccaacctt cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg
     4561 attccagcgg tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgttg
     4621 actggagctt ctccggcacc acggaagaag agttcgccac gctgatggct atcttcaaaa
     4681 aggaagataa agaagtttat attgccgatt acgagcatct gggtgtctac tcttgccgta
     4741 ttatcgtacc tggcatgtcc gatatttatc cggctgaaga tctgtggttg gcgaataaca
     4801 gtatgggcag ccatttacgt gaaacgattc tgtcactgcc cggtagcgag tgggaaaagg
     4861 aagattacct gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg
     4921 tgcgtgaact gttgggcctg gcgaccgggt cggataacgg ctggtacact ctgcgtatcg
     4981 gtgaattaaa agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga
     5041 ccgaatggac gatggagttt aactcatcag tattcagccc ggaacgcgcc aattattatc
     5101 gctgcctgca aacattgtta ttactggcgc aggaagaaga tcgccagccg ctgcaatatc
     5161 tgaatgcgtt tgttcgcatg tacggcgcag atgccgtgga agccgccagt gcagcaatga
     5221 gcggcgaagc ggcgttttat ggcttgcaac cagtagatag cgatctgcac gcgtttgctg
     5281 cacatcagtc gttgttgaag gcctacgaaa agctgcaacg cgccaaagca gcattctggg
     5341 caaaataaaa tcaaatagcc tacgcaatgt aggcttaatg attcgtctga gttatattac
     5401 gg
//
LOCUS       DAJOTB010000232         5333 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJOTB010000232 DAJOTB010000000
VERSION     DAJOTB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.232"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            290..1141
                     /locus_tag="OHN28_004611"
     CDS             290..1141
                     /locus_tag="OHN28_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCQ2299878.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1168..2157
                     /locus_tag="OHN28_004612"
     CDS             1168..2157
                     /locus_tag="OHN28_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCQ2299879.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2188..3081)
                     /locus_tag="OHN28_004613"
     CDS             complement(2188..3081)
                     /locus_tag="OHN28_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299880.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3281..4207)
                     /locus_tag="OHN28_004614"
     CDS             complement(3281..4207)
                     /locus_tag="OHN28_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299881.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            4364..5284
                     /locus_tag="OHN28_004615"
     CDS             4364..5284
                     /locus_tag="OHN28_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2299882.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1470 a         1281 c         1204 g         1378 t
ORIGIN      
        1 gtgaataatt cattatccag agtgaataat aatgacccgt ctttaattaa ctgctattgg
       61 gagtacgtcg aagtaagtat atacgccggg ataaaccaat atatttttta tggacattca
      121 tttttattca tcggactgta aattccgggg atatcgaact tgcacgacta atctcaaccc
      181 tcaaacagat ttatgaattt tgttcataac ccctgacaaa tcctgccgtc taatcgagct
      241 cttcccttta cgttatgctt ctcatgaacg ataacggaag gataacacca tgcaaaaagt
      301 aaaactgaac aacggtattg aaatgcccct gttgggcttt ggtgtatttc agatgaccga
      361 tgccgccgaa tgcgaaagag cggtcattga cgctattaac agcggttatc gactgattga
      421 caccgccgcc tcttatcaaa atgaaatcca ggtcgggaac gcactgaaac aaagcggtat
      481 cgcccgtaac gaactctttg tcaccacgaa actctggttg caggatacca gttacgaagg
      541 ggccaaagcc cagttcgaac gctctctgaa tcgactgcaa cttgattacg ttgacctgta
      601 cttgattcac cagccatatg gcgatgtcca cggagcctgg cgtgccatgg aagagctgca
      661 acaggccggt aaaattcgcg ctattggcgt cagtaacttc catcctgacc gactggcaga
      721 ccttatcgct tttaataacg tcgttccggc ggtaaaccag gttgaagtta accctttcaa
      781 tcagcagcta caggccgttc cctggatgca aagtcgtggc attcagccag aagcctgggc
      841 accgtttgcg gaagggaaaa atggtctgtt ccagcatcct gttctcacgg caattggcga
      901 gaagtatggc aaaagcgtgg gccaggttgt tctgcgctgg atctaccaac gaggcattgt
      961 ttcactggca aaatcagtgc gaaaagaacg catggaagag aacatcaaca ttctcgattt
     1021 tgaactcagc cctgaagata tgctgcaaat taccgccctg gataccgcaa ccagcgcgtt
     1081 cttctctcac cgcgatcccg caatggtgga atggctgact ggccgcaaac ttgatgttta
     1141 agccgcgata aaagaggtat tcattcaatg caaaaacgtt atctgggtaa atccggactc
     1201 gaagtctccg cccttgggct cggttgcatg ggcttaagcc acggctacgg cccggcgacc
     1261 gatacccgtc aggctatcga acttattcgt gctgcggttg aacgtggtgt caccttcttc
     1321 gataccgccg aagtgtatgg tccgtatctg aatgaagaag tggtcggcga agccttaaaa
     1381 ccgtttcgtg accgcgtggt aattgccact aaattcggtt ttaccttcgg cgatgataat
     1441 aagcagcaga ttttaaacag ccgtccggaa catatccgtg aagccgttga aggatcatta
     1501 cgtcgtctga aaacagatgt tattgacctg ctgtatcaac accgtgttga tccagatgtt
     1561 cctattgaag atgttgcagg aacggtgaaa gacctcatcg cagaaggtaa ggtcaaacat
     1621 ttcggcctgt ctgaagcagg agcacaaacc attcgtcgtg cacatgcggt acaacctgtc
     1681 accgccctgc aaagcgaata ttccatgtgg tggcgtgagc ctgaacagga aattctgccg
     1741 ttgctggagg aactgggaat tggttttgtc cccttcagcc cactaggtaa aggtttcctg
     1801 acaggggcga ttaagccggg aaccactttc ggcaaagatg attaccgcag caccgtaccg
     1861 cgtttcgcag cacaagccat tgaagccaat gaaaaactgg tcaccttgtt aggtgaactg
     1921 gctgcagata aaggggtaac gtctgcacaa atcgcactgg catggctatt agcacaaaag
     1981 ccgtggattg ttcccatccc tggcaccact aaactgaacc ggctggaaga aaacctggcc
     2041 gctgctgaca ttgttctttc gcagaaagat acgcagcaga taaccgaggc gctggaaaca
     2101 attaaaatcg tcggtgaacg ttactcgcct gaacatcagg cacgcgtagg ccgctaatac
     2161 ccaaacgggt ccaggataaa ggacccgtta ttcggtgtag cgaagcgcat cgatcaacag
     2221 cgcaaaagct ggcggatgct gcttgcggct ggggtagtaa agataatacc caggaaaagt
     2281 tggacaccac tcctgtaaca cctgaataag cttgcctgac tttatatact cctgaaccat
     2341 gtcttcaggg acacaagcga tcccaaaccc tgataacgcg gcatcaattc tttctggcaa
     2401 tggactacac gtcagttgcc catcaacctt aacgcgaagc ggtttccctt ctcgctcgaa
     2461 ctcccagtgg taaagcccac cggcagttgg caaacgcatg ttgatgcagc gatgattttg
     2521 tagctcatga ggcgtttcag gcgcagggtt tgcagcaaaa tacgccggag ctcccaccac
     2581 agccatccgc atatctggtc caatcctgac tgcaaccata tctttatcaa cattttcacc
     2641 cagtcgtatc ccggcatcaa aacggccttc aacaatatcg acaaaaccgt tatccaccac
     2701 cagttcgaca ttgatttcgg gatactctct gagaaaaggt tttagcttcg gccagaccag
     2761 acttcgcgcg gcatgttccc cggcagataa ccgaatattc ccggagggcg tgccgttcaa
     2821 ttgaataagt gattccagtt cctgttcaag ctcgtcaata cgcggttcaa ggcaggcaat
     2881 gattctctcc cccgcttctg tgggagccac actgcgggta gtccgtgtca ggaggcggat
     2941 attgagcctt tcctccagag ccttcattgc atggctaagt gcagactgag agacgccgag
     3001 tttgcccgct gctttggtaa aacttcgctc tcttgccacc acaagaaaga tttgcagttc
     3061 gttgaagttt tctttgagca ttggcgattt ccttgtagaa gcatcctctt cacagacact
     3121 catgaagggg ggcatagatg ctattatttt aaccacatta ttagtaacaa tgataatctg
     3181 gctgtatcga aaataattct aaactttcag gagatttatt atgaataaac gtattctcac
     3241 gattgcgcca ttcttcttac cgatattctg acacacatca ttaactttct atttttactc
     3301 ttttctccac cataaaatca ataaaagcac gaactttaga gggcattctc acccgagatg
     3361 aaacatatat ataaaatcca ggaaatgaaa cggcccaatc ctcaagaatc ggctgtaact
     3421 tgcccgcatt gatatgttca gcgattgcaa aatccagatg ttgaatgata cccgttccat
     3481 tcagcgccat tcgtatcatg ctttcgtcat cattagaaat atacttacct gacacctcta
     3541 tttcgaactc ttcattaccc cccacaacat tcgaaaactc ccatgctgag agtgcaccgc
     3601 tacttttatg ccgcaaccga agacaattat ggttactcaa ttcctgagga gtttcaggaa
     3661 taccgtgttc ttttaaataa tctggcgatg ccacagtaac cagtttaata agcggagaaa
     3721 caggaatggc agtcatatgc tcatccagtc cttgctcaag acgtatgccc acatcacagc
     3781 cgtccgcaat aatgttagat aaaccgtcat ccataatgag ttctatcttt gttttggggt
     3841 aacgagtaag aaactcgcca atatgaggct caacaagcat ccttgctgca acacgggagg
     3901 aatttattct caacagtcct gtaggttcaa gttgggtatc accgacatct ttcaacgcat
     3961 catcaatttg ccccaaagcc gacaccagca cttcatacag atgctgtcct tcctcagtaa
     4021 gcgacatatt gcgggttgta cggtatatca gcttggtgtt aagacgttcc tcaagagatt
     4081 ttaaattctg ggacaatgcg gcccgcgtaa tacccatttc agaggctgct ctggaaaaac
     4141 tgccggcacg aacaatcagc gcaaaccagg caagggaagg caaaattgaa ggctctattt
     4201 tcatcattgt aaaccttaac ttaacactgt gatagatatt agaccattga tgataaccaa
     4261 tacaagtcat actattacgc cgttcgcgat atgaacacga atgccagttg cggataatag
     4321 aaatgatatt taaatctgtt taggcaaatc cctggagctt gaaatgaaaa ctgtttcaat
     4381 aaaacacact tactgggata tagctgctga tatctatttc cctgacgatt ttaatagtga
     4441 aaaaaagtat cctgccatta tcagtgccca tccaattgga agctgcaaag aacagacatc
     4501 gggtagtgtg tatggtgccg ccctggcaaa agctggcttt atcgtcattg cttttaatgc
     4561 cagttttcag ggaagcagcg gtggagaacc gcgttatctg gaagatccaa caatgcgagt
     4621 aaaagacttc agcattgtcg ttgattacct taccacctta ccttacgttg atgccgggcg
     4681 tattggtgta ctgggtattt gtggtggagg tggatatgcc attaatgccg caatgacaga
     4741 acgtcggatt aaagccattg gtactgtcac aggtgcaaac tacgggcgtc ttatgcgtga
     4801 gggatttaca gcgtttaatc ctatcggagc actcgaagca atggcgcaac aaagaacaga
     4861 cgaagctaac ggcgcaaaac ttcgcgtaga tgatttgctt ccttcttctc cggaagcagc
     4921 tcttgaagcg ggattaacag aaatagacct ttttgaagca acagaatatt atcgttcgcc
     4981 acgaggctgt gcgcctaatg gggtaaatcg ttcgctattt tcacatcaga ctgttgccgt
     5041 gggctgggat gcttttcatc ttgcagaagt cctgttaact caaccactaa tggttgtagt
     5101 aggcgataga gtgggcgctt ttggtgccta tcgtgacggc tgcgaaatta ttggtcgtgc
     5161 agcctcaaaa cataaagagt tggtcgtggt tgaaggttat tcacattacg acctgtatga
     5221 taagccagaa cctgtgaaac aggcgctcga taaattaatt cctttttaca aaacacattt
     5281 gtaataacct tgcagggaga ttcattatct ccctgacatt tatcctttat tga
//
LOCUS       DAJOTB010000233         5307 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJOTB010000233 DAJOTB010000000
VERSION     DAJOTB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.233"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            660..911
                     /locus_tag="OHN28_004616"
     CDS             660..911
                     /locus_tag="OHN28_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCQ2299883.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYC
                     VPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
     gene            901..1182
                     /locus_tag="OHN28_004617"
     CDS             901..1182
                     /locus_tag="OHN28_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2299884.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARL
                     HGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
     gene            complement(1461..2660)
                     /locus_tag="OHN28_004618"
     CDS             complement(1461..2660)
                     /locus_tag="OHN28_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCQ2299885.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITT
                     ESQWRSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDIS
                     LLRYMPA"
     gene            complement(2670..2858)
                     /locus_tag="OHN28_004619"
     CDS             complement(2670..2858)
                     /locus_tag="OHN28_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299886.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSI
                     LKRRGDTAALTLLQDTGV"
     gene            3095..3853
                     /locus_tag="OHN28_004620"
     CDS             3095..3853
                     /locus_tag="OHN28_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCQ2299887.1"
                     /translation="MKKQIMVAFVASLIVISGAQAGIPVAIDANPEWAIEAGRWTERL
                     KQWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDY
                     IKNPEGALSEQAKSLLSDYKVTDTCQNLGYSGDLVRGCEATFLSQLASVEYGNKLESK
                     LRQDNQTMKDLIDQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYRDKQRDLAEY
                     KEKMAQAAFRKQQREAVPPSYRKAYMAMKSYEDD"
     gene            3854..4081
                     /locus_tag="OHN28_004621"
     CDS             3854..4081
                     /locus_tag="OHN28_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HCQ2299888.1"
                     /translation="MKSIATAGLVIISVFSLSGCFEETKSVDWWLAHPKETYKKFEEC
                     QKSGSDSDNCKNVKRAHLSFERRKAVGLPIN"
     gene            4091..5224
                     /locus_tag="OHN28_004622"
     CDS             4091..5224
                     /locus_tag="OHN28_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2299889.1"
                     /translation="MGIVTGIPDGTDLSGNVTSPANAGGVSGFDSDFFQTTHEVIFNI
                     LNKSISGKLSEYSDVAYTLGKYGVSLYVLWYAFTVLARKQQTPVPDFIWNICRFYIIL
                     LFVKNTGGILTSATDAIDGLKNTLAGGDPWVWMDQLWVKVIQVATLIFDKDTSTVPVA
                     GGIGALLTYVGGVLALLLCSIVFASAELTLLLLSVTAPIFIMCLMFGLLRQMFNSWLQ
                     LNFSSLLVFLFAALALRAGTWQLNMALSTSIATASENNLLQTGVTSLAAGIFMAWIIW
                     QAKSYASQIAGVGVEGAMQGAAAMGIGAGVFGVSRMARGALGMGRNAGIGAWKGLRGQ
                     EGGFGQSPGITGKTANLAGQGVNLGAKKLRQAAIERAKKMYGG"
BASE COUNT         1404 a         1200 c         1314 g         1389 t
ORIGIN      
        1 cagtgcctgt ggataacttt gcgtaaaccc ggaggacaga tcactctgaa ccagcacaac
       61 cacacataaa aacaatacgc tcgcttttta accaccgcgc gtaagtgcct aatgttagtc
      121 ctgtgctgtt ccgcttcctc gctcactaac tcgctacgct cggtcgttcg gctgcggcga
      181 gcggtgtctc ctttctcaaa accagaagag ttcagatcat acgatccgtt gattgatcct
      241 ttttggatct ttcactgaga agccctgaat cgctttctgc cgcttcgttc gatacaacca
      301 tggtgaaacg atgcgaaatc gaaaaaatcg catcagaatg cgatacagcg cgttttagag
      361 ggtatttaca aaacagtcgt tgccttgagg aaattttgat caagattgaa ctcctatccg
      421 gatcatggca acagatcaca gcaacaaggc cgctaaaaag cgttgagcgg cgttaaaaac
      481 cgttaaaccg tagctgacta caacgaaacg ggaaaaaacg ctcagaacgc gttacagagc
      541 gttttaaggc gatgtcggaa agagccatat ctcacctgtt ctgatgtttt tctcaacaca
      601 agcactttta agagtgcttt taaagatgct attatgccaa tgagacaagc ggagggctta
      661 tgagctatca aattctgaca accacagcgg ccagtattac tgacctgaaa aaaaatccta
      721 tgggaaccgt agctgaaggt gaaggggacg ctgttgcgat cctgaaccga aacgaaccgg
      781 cgttctattg cgttccacca aaactttacg cctactatcg ggaactcgct gaagatgctg
      841 agttaaacgc tgttgctgat gagcgcatga aaaacccgga aattgtgaag gttaacctgg
      901 atgacctatg aactggcttt tgaccgtagg gcactgaagg aatggcagaa actcggccac
      961 accatccgtg aacaattcaa aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc
     1021 gcagcccggt tacatggtca tgctgatcgc tataaaatca aacttcgtgc atctggctac
     1081 agacttgtat atcaagtcat tgatgagaaa gtcgttttac tcgttattgc cgttggaaga
     1141 agggaaagca gcgaagtcta tcagatcgca gatttgcgct aagattaatc atcaaatgat
     1201 tgactgttta cagtcagagc gagagcatta aaaatttgcc atacctgcaa atgcaggtat
     1261 aataaaacac aagaaaggga aacaggttca cgtataggaa attgaaaaac cagtcgatac
     1321 cattgtccac gccttatgcc acgtgagcct ttctgctttt cccaccactc cggtggtttc
     1381 cctctctgta tggtcgattt ttataccgta acaggctgtt ttttatactt tcaccgattc
     1441 ccgggcacac ttagcctgtg tcaggccggc atatacctca gtagactgat atcccgcgcg
     1501 tttttcttca gccctgatac attcagcccc gcaatggccc ggcggtatac catccggcag
     1561 ccacacagca cgcattcgaa cgggtcacga ctcaggaact gtttcaccat ttgtgcatag
     1621 cacactttcg ccactggttc cggtttatcc atccccagtg cacggtacac ctgcggcagc
     1681 ttctctccac acacacggtt ggcaaggaac ccgaagtacc tcaccatctt aaaaaacttc
     1741 tccgggatgt gctgtttcag cctcgcgacc agctcacgct gtgtcagcgt ttccgtcgcc
     1801 gtttctcccg ttttgtggtc caggtaacgg aagctcaggc ttgcccctcc gttgtaatga
     1861 gccagtcggg aagccgctat tggtggcttc ttcagataac gacccaggta gcgtgccgta
     1921 ttccgccctc cggctgtctt cttcgacatg tacacatgcc agtattttcc gccggatttc
     1981 agcaccaggc ttctccactg tgattccgtc gtgatatgtg acaacgactc cggcattgcc
     2041 atcccctctg accacgcttt cagaagcagc tgccgcatat tccacatcca ccgtgaacgc
     2101 atcgcgtctt tcaggaagct cagctttttc cactgaccat gcttattcag acctccacag
     2161 gttacagaca catgtacatg cggatgccag ttgagacgac ggccatacgt gtggatggcg
     2221 cagaagatac cgggttccag cccccgtttt cgggcggcat acagcagatt ctccaccgcc
     2281 agacggcaca cgtcattcag cagccagcgg ttgctttcga acaccggcca cagcgtgtcc
     2341 ggcagggtga agaccagatg tacccagtcg cagtcaggaa gacgattcag ctgtgttgct
     2401 gtccacaggt ctgtggcctt ctttccgcag gacgggcagg cacggctgcc gcatgagttg
     2461 gtcaggtact ttacgtgctg acagtccggg ttatcacacc cgaactcttt tacacccagt
     2521 atccgtgtgc cgcaggccag cattttggtg acggcttcaa cctcgatatc gcgcagaccg
     2581 cccgcatcca ggaaggacgt ccagcactgg ttggctgtga acagacgttt cagagggcgg
     2641 ggagtaaaac cggacaacat gaggatggat taaaccccgg tatcctgtag cagggtaagg
     2701 gctgctgtat cgccacggcg cttcaggatg gaggtcgtca ccatcgcagg tggtgggtcc
     2761 atgggtttga gcattttcat ccagtcccat tctcgttgtg agagcttctc agcaccaaca
     2821 gagagcagaa ctgtatcgat acttttgcgg gtcaacataa ggggatgagt ccttaataaa
     2881 acaggtcctc agagcataca tattctgcgg ggcgtgcggc aacaacagga cgaaccgggg
     2941 cggttttcaa tttgcagccg ccaggctgcc gtggttctca gtgcatcaaa accccagtta
     3001 gaactgttcg atgaaatctg gaaagaagga atgaagccgg aagagtggct tgatacctat
     3061 ctggaacagg cgaacctgat ttgaggaatt accaatgaaa aagcaaatta tggtggcatt
     3121 cgtcgcttca ctgattgtta tttccggcgc tcaggcaggg atacctgttg ccatcgacgc
     3181 caaccctgaa tgggcgattg aagccggacg atggacagaa cgccttaagc aatgggcgga
     3241 aacggtaaaa cattacgaaa atcagataaa tgcgtacaaa caggagctgc tgtcaaaaac
     3301 gggtatccgt gatgtgcagg gactggtgca gtccgcacag tcagtgagtc aggaactgat
     3361 gcagatttat gatcagggga atgcttttat tgacgattac attaaaaatc ctgaaggggc
     3421 gttatcggaa caggccaaat cgttattgtc agattacaaa gtaacggata cctgccagaa
     3481 cctgggatat tccggcgacc tggtacgggg atgtgaagcg acgttccttt ctcaactggc
     3541 aagcgtggaa tacggtaaca agctggagag caagcttcgt caggacaacc agacgatgaa
     3601 agaccttatt gatcaagtca aaaatgcgaa ggatacgaag gccacgcagg atgcaacaaa
     3661 cgctgttgca cttgaacaac tgaagttcga gaagctcaaa tttcagtatc aaatgtatcg
     3721 cgataagcag cgagatcttg cagaatataa agagaagatg gctcaggcag ctttcagaaa
     3781 acagcaacgt gaagccgtgc caccttctta cagaaaagct tatatggcaa tgaaatcata
     3841 tgaggatgat taaatgaaaa gtatcgctac tgctggcctt gtcattatat ctgtttttag
     3901 tctttctgga tgttttgaag aaacaaaatc ggttgattgg tggcttgcac atccgaagga
     3961 gacatacaaa aaatttgaag agtgtcagaa gtctggtagc gattctgata attgtaagaa
     4021 cgttaagcga gcacatttgt cttttgaacg gagaaaggcg gttggtttac caataaattg
     4081 agggtgatgt atggggattg tcactggtat accggatggt actgatttat caggtaatgt
     4141 aacctctccc gcaaatgcgg gaggtgtttc tggatttgat tctgattttt ttcagacaac
     4201 tcatgaagtt atatttaata ttctcaacaa gagtatatct ggaaaattaa gtgaatattc
     4261 agatgtggct tatactcttg gtaaatatgg agtttctctg tatgttttat ggtatgcttt
     4321 tactgtatta gcaaggaaac aacagacacc tgtacctgat tttatctgga atatctgtag
     4381 gttttacata atattgcttt ttgttaagaa tacaggggga atacttacat cagcaacaga
     4441 tgctattgat ggattgaaaa atacattggc agggggagat ccgtgggtat ggatggatca
     4501 gttatgggtg aaggttatac aagttgcaac tcttattttt gataaagata catctactgt
     4561 gcctgttgcc ggtgggattg gtgctttatt aacttatgtc ggtggtgttt tggcattatt
     4621 gctttgttct atagtatttg catctgctga attaacatta ctattacttt ctgtcactgc
     4681 gccaatattt atcatgtgtc tgatgtttgg tttacttcgg caaatgttta atagctggct
     4741 acagcttaat tttagctcgt tactggtttt tctatttgca gcattagcac taagagctgg
     4801 gacatggcaa ttaaacatgg cattaagtac gtctattgct acagcatcag aaaacaatct
     4861 tcttcaaacg ggagtaactt cattagctgc tggcattttc atggcctgga ttatctggca
     4921 ggcgaaaagt tatgcttcac agattgcagg tgtgggtgtt gaaggtgcca tgcagggcgc
     4981 agccgctatg gggattggtg ctggcgtttt cggtgtatcc cgtatggcgc gtggcgcact
     5041 tggtatgggc agaaatgccg gtattggtgc atggaaaggt ctgcgtggtc aggaaggcgg
     5101 gtttggtcag tctccgggaa taacgggtaa gacagctaac ctggccggac agggcgttaa
     5161 tcttggtgcc aaaaagcttc gtcaggcagc tattgagaga gcaaagaaaa tgtatggtgg
     5221 gtaatttaat atgagcgcct ttttaatgtt cttttcataa ttgtcattgt cgggtttggc
     5281 acgctctatt atcttttcct ttcacac
//
LOCUS       DAJOTB010000234         5298 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJOTB010000234 DAJOTB010000000
VERSION     DAJOTB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.234"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..900
                     /locus_tag="OHN28_004623"
     CDS             <1..900
                     /locus_tag="OHN28_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299890.1"
                     /translation="INQTLEQMVQALFKSWFVNFEPVKAKIAVLEAGGSQKDATLAAM
                     TAISGKDADSLAVFQREYPEKYAELRAIAELFPLAMQDSELGEIPVGWFCTDLSYLAL
                     LNNSSWSKKNAPESLKYVDLANTKWGIIHSTEDFLFSDAPSRARRILKPGDTIVGTVR
                     PGNGSYAFIAEEGLTGSTGFAVLTPKHEHFSSFIYICATSKDNIERLAHLADGGAYPA
                     VNAEVILSTPCIIPIDHSSSKNLIECFHYYVRELFAYKHNNLRQNKSLEQLRDTLLPK
                     LLSGEITLPDAEEIAKEADYVQS"
     gene            887..2080
                     /locus_tag="OHN28_004624"
     CDS             887..2080
                     /locus_tag="OHN28_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HCQ2299891.1"
                     /translation="MFKVDQPSNSLQPLQETTFTNAGFTERYHLQEWLAKHPQALTRS
                     SDDELLIIQKEFAGFDDTKERLDLLAIDKDGNLVIIENKLDDSGRDVVWQALKYAGYC
                     ANLRKEHIVEIFQRYLDQNDPEESRPASDVLAEFLEHESLDEVQINQSRSQRVMMVAA
                     NFRKEVTNTALWLMQFNIRVQCFKVTPYRYGNDVFIDIRQVIPTPEAESYMIGMAQKE
                     AEEQSTSGELKQSQRLRKAFWTQMLEKFHASDCTLYNNISPSMDHWLCAGSGISGMPY
                     SLIFSKKEIRVELWISRASADINTMALNWLEQHRTSVEEVFGNPLEWLSLPGKKACRI
                     QYRQKIDGFQQENWPQMIDWLVEHMTRFENALSPYLPQLNQLIKQTFNGHDEAENTTE
                     EAEHV"
     gene            2096..5230
                     /locus_tag="OHN28_004625"
     CDS             2096..5230
                     /locus_tag="OHN28_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCQ2299892.1"
                     /translation="MLTEDDLEQLCLGWFAEQGWEIHHGPDIAPDGSNPQRASYHDVF
                     LTPVLNACLQKINPHLPADVFEQVISRITRAESPDLIASNKTFHHWLLEGVPVQYRRD
                     EELIHDHALLVDFSQPQNNRFMVVNQMTISGTKQPRRPDIICFINGIPLAVVELKSPV
                     DDKVDIWDAYHQLQTYKDEIRDLFISNEALIVSDGYLARVGSLTATEERFMPWKAISN
                     ENDKPLLEWQLETMVRGFFNRELLLDYIRYFVLFENNDERLIKKIAGYHQFHAVREAV
                     QATIVASTGKQLVLHSNIQPGSKKAGVVWHTQGSGKSISMCCYVSKLLQQPEMGNPTI
                     VVVTDRNDLDGQLYGTFCQAQELLKQMPVQADDRETLRDLLATRDAGGIIFTTVQKFS
                     PEEGENAHPILNGRSNIVVISDEAHRSQYGMKANLDRKTGVYKYGYAKHMRDALPNAS
                     FLGFTGTPVSAEDRDTRAVFGDYVSIYDIQDAVDDGATVPIHYESRLAKLDLNHEELA
                     ALSDQVEELIEDDELDQREKTKGEWSRLEKLVGAKPRLQQIAADLISHFEKRNTVMDG
                     KTMIVAMSREICVHLYDEIIALRPEWHDDDPEKGQIKIVMTGTASDKAMLQPHIYNKR
                     TKKRLESRFKNVNDPLKLVIVRDMWLTGFDAPCCHTMYIDKPMRGHNLMQAIARVNRV
                     FKDKPGGLVVDYIGIANELKLALKTYTDANGKGDATVNAEEALAIMLEKLDIIHGMFA
                     KTATSAGFDYTGFEAHAQKLLVPVANYVLGLEDGKKRFLDNVLAITKAWSLCCTLDAA
                     QPYKLEIAFLSAVKAAISKFTSVDKKISQAEKNSALKQILDNAVIANGVDDIFTLAGL
                     DKPNIGLLSDEFLQEVRDMPYRNLAVELLEKLINDGIQSRTKNNVVQERKYSERLQAV
                     LIKYNNRAIETAQVIEELIQMAKDFQSAMERDDALGLNPDEIAFYDALAENESAVREL
                     GDEVLKKLAIEVTLKLRQSTTVDWQVRESVRARLRILVRQTLRRYKYPPDKTPGAVEL
                     ILKQAEVISNHWTM"
BASE COUNT         1488 a         1228 c         1324 g         1258 t
ORIGIN      
        1 ataaatcaaa ccttagaaca aatggtacaa gcccttttca aaagttggtt tgtcaatttt
       61 gaaccggtaa aagccaaaat cgcggtgctg gaagcaggtg gttcgcaaaa agatgcaacg
      121 cttgctgcca tgacagcgat ttctgggaaa gatgctgatt ctttagctgt atttcagcga
      181 gagtatcctg aaaaatatgc cgaacttaga gctatagcag agttgttccc gctagcgatg
      241 caagacagcg aattaggaga gattcctgtt ggatggtttt gtacggactt atcatatctt
      301 gcattactta ataactcttc ctggtcaaag aagaatgctc cagaatcatt aaagtatgtt
      361 gatttagcaa acacaaaatg gggcattatt cattcaactg aagatttctt attttctgat
      421 gcgccaagtc gtgcccgtag aatattaaaa cccggtgaca ctattgtagg aactgtgagg
      481 ccaggaaatg gttcatatgc ctttattgct gaagagggcc tgactgggag caccggtttt
      541 gcagtattaa cgccaaaaca tgaacacttt agttcgttta tttatatctg tgcaacgtct
      601 aaggataata tcgaacgtct ggcccattta gccgatggcg gagcatatcc agcagttaat
      661 gctgaggtta tactatctac tccgtgtatc attcctattg atcatagcag cagcaaaaac
      721 ctaatagaat gcttccacta ttatgtcaga gagttatttg cttataagca taacaatctt
      781 agacaaaata aatcactgga acaactccgc gacaccctcc tccccaaact cctctccgga
      841 gaaattacgt taccggacgc cgaggaaata gccaaggaag ctgactatgt tcaaagttga
      901 tcagcccagt aatagcctgc aaccgctcca agaaaccacc ttcactaacg cgggtttcac
      961 cgaacgctac cacttacagg aatggttggc gaagcatccg caggcattga cccgcagcag
     1021 cgatgatgaa ttacttatta ttcagaaaga atttgccggg tttgacgaca ccaaagagcg
     1081 gttagatctg ctggcgatcg acaaagacgg taatctggtg atcatcgaga acaagctcga
     1141 tgattccggg cgtgatgtcg tgtggcaggc gctgaagtac gcgggctact gcgcgaatct
     1201 acgcaaagaa catattgttg agatcttcca gcgttacctc gatcagaacg atccagaaga
     1261 aagccgcccg gcttctgacg tgctggcaga attccttgaa catgaaagcc tggatgaagt
     1321 gcaaattaac caaagccgtt cccagcgcgt aatgatggtt gcggcaaatt tccgcaagga
     1381 agtcactaat actgcgctct ggctgatgca gttcaatatc cgtgtccagt gctttaaggt
     1441 cacgccttac cgttatggaa atgatgtatt catcgacatc cgccaggtta ttcccacacc
     1501 ggaagccgaa tcctacatga tcggtatggc gcagaaggaa gcagaagaac aatcaaccag
     1561 cggtgaactg aaacagagcc aacgcctgcg caaggccttc tggacgcaga tgctggagaa
     1621 atttcatgcc agcgactgta cgctgtataa caacatcagc ccgtctatgg atcactggtt
     1681 atgtgcaggc tctggcataa gtggtatgcc ttatagccta atattcagca agaaggaaat
     1741 ccgcgtagaa ttatggattt cgcgagcatc ggcagacatc aacaccatgg cgttgaactg
     1801 gcttgaacaa caccgcacat ctgtcgaaga ggtattcggc aacccactgg agtggctatc
     1861 gctacctggt aaaaaagcct gccgtattca ataccgccaa aaaatagatg gcttccagca
     1921 ggaaaattgg ccgcaaatga tcgactggct ggtggaacat atgacccgtt ttgagaatgc
     1981 gctttcgccg tatctgccac agcttaacca gttgatcaag cagaccttta acgggcatga
     2041 tgaagctgaa aatacaacag aagaagccga acacgtataa cacaggacgt taccgatgct
     2101 taccgaagac gacttagaac agctttgcct gggctggttt gccgaacaag gttgggaaat
     2161 tcatcacggg ccggatattg cccccgatgg cagtaacccg caacgcgcca gctatcatga
     2221 tgtattcctg acacccgtgc tgaacgcctg cctgcaaaaa atcaacccgc atctgcctgc
     2281 tgacgtcttc gaacaggtca tctcccgtat tactcgcgct gaaagcccgg atctgattgc
     2341 cagtaacaaa actttccatc actggctgct ggaaggggtt ccggtacagt accgtcgcga
     2401 cgaagaactc atccacgacc acgctctgct ggtggatttt agccagccgc aaaacaaccg
     2461 ttttatggtg gtcaatcaaa tgactatcag cggaacgaaa cagcctcgcc gcccagatat
     2521 catctgcttt attaacggaa taccactggc ggttgtggaa ctaaaaagcc ctgtcgatga
     2581 caaggtagat atctgggatg cctaccatca gttacagacc tataaagacg aaatacgcga
     2641 cctgtttatc agcaatgaag cgttgattgt cagtgatggt tatctggcgc gtgtggggtc
     2701 cctgaccgcc acggaagaac gttttatgcc gtggaaagcc ataagcaatg aaaacgataa
     2761 gccgttgctg gaatggcaac tggaaactat ggtacggggt ttcttcaacc gggaattgct
     2821 gctcgactat atccgctatt tcgttctgtt tgaaaataac gatgaaaggc taattaagaa
     2881 aatagctggt tatcaccagt tccacgcggt gcgtgaggcc gtgcaggcga cgatcgttgc
     2941 ttcgaccggt aagcaactgg tactgcacag caatattcag ccaggcagta agaaagcggg
     3001 cgtggtctgg catacgcagg gttccggtaa aagcatctcc atgtgttgct atgtcagcaa
     3061 actgttgcaa cagccggaga tgggcaaccc gacgattgtt gttgtgaccg atcgtaacga
     3121 tctggatggc cagctttacg gtacattctg ccaggcacag gagctactca agcaaatgcc
     3181 ggtacaggca gatgaccgtg aaacattgcg tgatctgctt gcaactcgcg atgctggcgg
     3241 cattatcttt accaccgtgc agaaattctc cccggaagaa ggggaaaacg cccatccgat
     3301 tctcaatgga cgcagcaata tcgttgttat ttccgatgaa gcgcaccgca gccagtacgg
     3361 catgaaagcc aacctggatc gtaaaaccgg cgtctataaa tacggttacg ctaaacatat
     3421 gcgcgacgcg ctacctaacg cctcgtttct gggatttacc ggtacaccgg tttcggcaga
     3481 ggatcgcgat actcgcgctg tatttggcga ttacgtctca atttatgata ttcaggacgc
     3541 tgtggacgat ggcgcaacgg tgcctattca ctatgaatcc cggctggcaa aactcgatct
     3601 caatcatgaa gagctggcgg cgctgtccga tcaggttgaa gaactgattg aggacgatga
     3661 gctggatcag agagaaaaaa ccaaaggtga atggagccgc ctggagaaac tggttggtgc
     3721 taaaccacgt cttcagcaga tcgctgccga tctgatttct cactttgaga agcgcaatac
     3781 cgtaatggac ggcaagacga tgattgtcgc catgagccgt gaaatctgcg tccatcttta
     3841 cgatgaaatt attgcgttgc gtccggagtg gcacgatgac gatcctgaaa aagggcaaat
     3901 caaaatcgtg atgaccggta cagcatctga taaggcaatg ctgcaaccgc atatctacaa
     3961 caaacggact aaaaaacggc tggagtcccg ctttaagaat gtgaacgacc cgctgaagct
     4021 ggtgatagtg cgcgatatgt ggctgaccgg ctttgatgcc ccttgctgtc acactatgta
     4081 tatcgacaaa ccgatgcgcg gccataacct gatgcaggct attgcccgtg tcaaccgggt
     4141 attcaaagac aagcccggcg ggctggtggt ggattatatt ggtatcgcca atgagctgaa
     4201 actggcgctg aaaacttata ccgatgcaaa cggtaaaggt gatgcgaccg tcaacgctga
     4261 agaagcactg gcaatcatgc tggaaaagct ggacatcatc cacggtatgt ttgccaaaac
     4321 ggcaacgtca gcgggttttg attacaccgg ctttgaagct catgcgcaga agttgctagt
     4381 accagttgca aactatgtgc tcggccttga agacggtaag aagcgttttc tcgataacgt
     4441 actggcaatt actaaagcct ggtcgctttg ctgtacgctg gatgcggcac agccttataa
     4501 actagagatt gctttcttgt cggcggtaaa agccgctatc agcaaattca ccagcgtgga
     4561 caaaaaaata agccaagctg agaagaactc cgcgctgaaa cagattctgg ataatgccgt
     4621 tattgctaac ggcgtggatg atattttcac gctggctggt ctggataaac cgaatattgg
     4681 cttgctttca gatgaattct tgcaggaagt ccgcgatatg ccatatcgaa atctggcggt
     4741 agagctgctg gaaaaactga ttaatgacgg tatccagtcc cgtacaaaaa acaatgtagt
     4801 gcaggagagg aaatattcag aacgtcttca ggctgtattg attaaataca acaaccgggc
     4861 tattgagacg gcgcaggtga ttgaagagct gatccagatg gcgaaggatt ttcagtctgc
     4921 aatggagcgt gacgatgcgc ttggcctgaa tcctgacgaa atcgctttct acgatgcact
     4981 ggcagaaaat gaaagcgccg tccgggagtt aggagatgag gtacttaaga aactggcgat
     5041 agaagtcacc ttaaagttga ggcaatccac taccgttgac tggcaggtgc gcgagagcgt
     5101 gcgtgcgaga ttgcgtatcc tggttcgcca gactctgcgc cgttataagt atccgcctga
     5161 caaaacgcct ggggcggtag agttaatttt aaaacaagcg gaagttattt cgaatcactg
     5221 gacgatgtaa atttcatgtt gacagcttct tgctgtcaac atcgttactc attttttgtg
     5281 ataatatgaa tacagcac
//
LOCUS       DAJOTB010000235         5280 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJOTB010000235 DAJOTB010000000
VERSION     DAJOTB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.235"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..1008
                     /locus_tag="OHN28_004626"
     CDS             163..1008
                     /locus_tag="OHN28_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCQ2299893.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(1038..3035)
                     /gene="tkt"
                     /locus_tag="OHN28_004627"
     CDS             complement(1038..3035)
                     /gene="tkt"
                     /locus_tag="OHN28_004627"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2299894.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(3086..4594)
                     /locus_tag="OHN28_004628"
     CDS             complement(3086..4594)
                     /locus_tag="OHN28_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCQ2299895.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4636..5193)
                     /locus_tag="OHN28_004629"
     CDS             complement(4636..5193)
                     /locus_tag="OHN28_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCQ2299896.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
BASE COUNT         1406 a         1431 c         1177 g         1266 t
ORIGIN      
        1 gagcgcccgt ttaaattatt tttgtgcagt tgacgtaatt catgaaaatt aaccggtctg
       61 ttaatcatta cgttcactgc acaccttcat tttttcacac ctatccacgc ctgaaaacta
      121 tgttataatc agcactttct gtcagaatga aatttactaa ttatgaataa cataaccgta
      181 aaaagtctga ttcaaagcaa ttaccctaca ctgcatcagg cggaaaaaaa ggtcgctgac
      241 tatattctta gccacgcaca tgaagtcgtg aactatagtg tcactgaact atcagaaaaa
      301 agccatgcca gtgaagcgac tatcgtacgc acctgtaaga aattaggtta tcagggttat
      361 taccatttaa aaattgcatt agcaaaagaa gtcattaatc ctgacaatag ctatcctgaa
      421 aataccgact tcagcgatat aacatctttg gcaacctttt tattaaaaaa acaggccgaa
      481 gatttaatac aatcaacgca attttttaat gccgacgtgc ttgagtccat tctcaaacta
      541 cttgctaatt gcgatactat tttctttttt gctgccggaa attcaaaccc actggccgtt
      601 tatagtgctt ataaattcag ccaactcgga ttaaaaaccg ttgttcacgt cagcccggaa
      661 atgcaaatta atgcggcata ttctatgggg aaacgcgacc tggcgattgg tatttctaac
      721 tctggcagca ccaacttaat ggtcgatatc tttaaagtgg tgaaagagcg cggtgccaaa
      781 tccgtttgta tcactaacta tattaaatct ccgttatcaa aactctccac ccatcaactc
      841 aataccgcgg tcagcgataa aatcttcttt gaagccttcg actccactcg cgttccggca
      901 atgggcgtta ttgatatgct ggtgctgttg tttatgtata aaaacaaggc gcattacgag
      961 aaataccgca cccgtgaaga gtttcttggc tcccgtttca aggggtaaaa ggtcatcctt
     1021 tcaccccttg tgcaggttta gctgttcagt agctcacgcg cctgcgccag gacattttcc
     1081 ggcgtaaaac caaagtgttt gaacaacaca ttcgccggag cagactcacc aaagctcgtc
     1141 atgccgatca ctttaccgtc caggcccacg tagcgttccc agaatccggc gatgctggct
     1201 tctaccgcca gacgtttgcg tacagctttt ggcaataccg actctttgta cgccgcatcc
     1261 tgattatcga aacgctcggt acacggcatg gaaaccacgc gaactttacg cccttcttcc
     1321 gttaacgctt ttgcggcgct gaccactaac tcaatctctg aacccgcaga aatgaggatc
     1381 atctcaggct gaccgtcaca atcactcagt acataaccac cacgcgcaat ctctgccaac
     1441 tgtgcagcgg tgcgcggctg ttgcgccaga ggttggcgcg ttaataccaa cgacgttggg
     1501 ccatctttgc gctcaatcgc ctgttgccag gccaccgcca cttccacctg atcgcaaccg
     1561 cgccaggttt ccatattcgg cgtcagacgc agcgacgcca gttgctcaac gggctgatgt
     1621 gtcgggccgt cttcgcccag gccgatggta tcgtgggtat aaacaaacac cgagcggatc
     1681 ttcatcagcg ccgccatgcg cagtgcatta cgcgcatatt ccataaacat caggaaagta
     1741 ccgccgtaag ggataaaccc accatgcagc gcaagaccgt tcatgatggc cgacatacca
     1801 aattcacgca cgccatagga gatgtaattc cccgccggat tttctccggt gaaatcaacc
     1861 gatttctgat ggcgagtcag gttagagggc gacaagtccg ccgagccgcc catcagttcc
     1921 ggcagcatat ctgcgaagtg attgaggcat ttctggctaa cctgacgggt tgccagcgcc
     1981 gccggatggg attgcagatc gtgcacatat ttctgcatat tctcgaccca gccagcaggc
     2041 aaatcaccct tcatgcgacg agtaaactcc gccgccagtt ccggccactg cttcgcgtag
     2101 tcggcaaaca gtgcatccca ttcctgttga acctgcgcgc ctttctcggt cgcatcccat
     2161 tcggcataaa tttcaccagg aatttcgaaa ggggcatacg gccattgcag acgttcacgc
     2221 accagcgcca cttcatctgc acccagcgcc gagccgtggc aatcgtggct gttggcttta
     2281 tttggcgaac caaaaccgat aatcgttttg cagcacagca gactgggctt atccgtgacg
     2341 cttttcgctt cacgcaccgc agcatctacc gcttctggat cgtgaccatc aacgccttca
     2401 atgacatgcc agccgtaggc acgaaaacgg gcagccgtat cttcagcgaa ccagccttct
     2461 acgtgaccat cgatcgaaat accgttatcg tcccagatag caatcagatt gccgagcttc
     2521 agcgttcctg caagaccaca ggcctcgtgg gagatccctt ccatcagaca gccatcaccc
     2581 aaaaataacc atgtgtgatg atcaacaata ttaaagcccg gtttgttaaa ttctgccgcc
     2641 agcgccttct ccgcaatcgc catccccacc gcattagcta cgccctgccc caacgggccg
     2701 gtggtggttt caacacccgg cgtataacca tattctggat gacccggcgt gcgggagtgg
     2761 agctgacgaa agtcgcgaat atcatccatc gacaggtcgt agcctgtaag atgcagcaaa
     2821 gcgtaaatca acatggagcc gtgaccgttc gactgaacat agcggtcgcg gttaaaccac
     2881 tgcggatttt ttgggttatg acgcaggtga cgacgccaga ccacttcggc aatatccgcc
     2941 atccccatcg gcgcgcccgg atgaccggag ttcgctttct ggatcgcatc aacacttaac
     3001 attcggatgg cattagccag taattgtgaa ttcatacttc agctctctct gtctggcggg
     3061 ctgaaaccag cccgctaatc tcgaattagt tagcacaggc cactgtcatt tctgtcgggc
     3121 gcggtctgct cattacggtt ttgatttcgt cgcgcaggat ttgcgatttg ccgccaaata
     3181 tcacctgtac accgccatca cccagtttca ctaaacccgc agcccccaac tccttcagcc
     3241 gtttttcatt cactttctta tcgtctacca gcgccagcct taggcgtgtt gcgcaggcat
     3301 ccacatcttc gatattgtcg aaaccaccta ctgcttcgat gatttggtat ggacgttctt
     3361 cgcgcgttaa tgtggttgca acggcaatct cttcatcctc acgccccggt gttttcagat
     3421 tcaacttgcg gatcagtacc cgaaatacgg tgtagtaaat cgctgcatag cccgcgcctg
     3481 ccaggaacac cagataccac ggcgtgcggt ttggcataac gccattcagt acgaaatcaa
     3541 taagaccgcc agcaaaagga tgaccaatgt gcacattcag gatgttcatc agcacaaatg
     3601 aagtggcata taagaagatg tgaatgatgt agagaaccgg cgctgcaaac aaaaatgcat
     3661 attccagcgg ctccgtaatc ccggtaacaa atgtggtcag cgcaccggag agtaaaaggc
     3721 ctttaatcag cgccttacgt tccggttttg cttcctgata catcgccaac gcggcggcag
     3781 gtaacgcaaa catcatgatc gggaaacgac ctgtcataaa cagccccgcc gtcaacggag
     3841 caccgtcgcg caattgggca aagaacaccg gaatatcgcc cgtcaccagt tgcccggatt
     3901 tggtcatgta gtcgccaaag ttgtaataaa acggcacgtt ccagatatga tgcaggccaa
     3961 aaggaatcag cgaacgttcg ccaacggcgt aaataaaggc cgcgaagttc gcgttatgtc
     4021 cactggtcac cagcgttgaa agcgacgcca gacctttttg taccggtatc cacagccacg
     4081 gcaataaata gccgacaata attgccgcca cagccgtaat aatcggtaca aagcgttttc
     4141 caccgaagaa accaagaaac tgaggcaatt caatattgtg gaagcgggta tagagcagat
     4201 aaacaaagat ggcgacaatc agaccgccaa acactcccgt ttgtaacgtc ggtaccccga
     4261 ggatcatcgc ataatcaccg ttactggctg caacggactc cgcagtaatg ccaagttgcg
     4321 ttcccatcgt ggcattcatg atcatgtaac tgataatcgc tgaaagtgat gaagaaccat
     4381 cacctgtcag gccaatcaca ataccgacac aaaacagcaa aggaaggttg gcgaaaatca
     4441 ggccgccgca attcgccagc agctcaatgt taaatgaaat cccgatggct aaaatcaaac
     4501 ccgccgccgg aagaatggcg attggtaaca tcagagcaga agaaaatttc tgcaacttct
     4561 tgaagttaaa caaggatgtt atttttttgc tcatttttat tctcctgtag ggtatcgagc
     4621 aaataacggc gacgactatt ccagattggc gtggcgctta aacagttcac tttccggcgc
     4681 accactttca gcagccatct tcatcactaa tgcatcggca atcaccagca aagcttgctc
     4741 atacaacgac gccatcggct gtgccgagcg gaaaacagaa tctttttgat ttttggaagg
     4801 ggcattgatt tgcacgatga cgtcgacaaa ctcggtgata gtggcgtgtc gattcgtggt
     4861 taacaccgcc acagcgccgc cgagctgacg tgcttttttc gccagcgcca ccagttgtgc
     4921 tgtctcaccg gaagcagaag aaacgataaa cagatcgcca ggcccaaagt tcggcgtaat
     4981 ggtttccgtc aggaagtaaa cctcttttcc catatgcatc aggcgcatgg tgaaggcttt
     5041 catagaaaaa ccggcgcgtc ccaggccata gcagaaaacg cgctggcttt gctgaatagt
     5101 gtcgatcaat ttggtgcagt catcctcacg gatagagtcc ttaacgaagg ttaattcatt
     5161 gatgatctca gtgatgtaca gctttgcgtc catttcacac ctcatgaaat aattttctca
     5221 aacacaaaat ctcatgaaat tttatttcat tccaatgcat cagtatgaaa tgtgaagcta
//
LOCUS       DAJOTB010000236         5190 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJOTB010000236 DAJOTB010000000
VERSION     DAJOTB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.236"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2452
                     /gene="tssH"
                     /locus_tag="OHN28_004630"
     CDS             <1..2452
                     /gene="tssH"
                     /locus_tag="OHN28_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCQ2299897.1"
                     /translation="DIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLIASLE
                     GDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPARQPQ
                     VTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVFGRDT
                     EIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRTLDLG
                     LLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAANLLKP
                     ALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKARYAQ
                     HHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLTQLHA
                     RLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKALAQQI
                     MTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRDEQSD
                     LLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKTETAL
                     TLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRPYSVV
                     LLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQLLEEK
                     PDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVRRLHQ
                     HYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHHKPAR
                     LRLGWDEEDGIVLEFDEK"
     gene            2464..4971
                     /gene="vgrG"
                     /locus_tag="OHN28_004631"
     CDS             2464..4971
                     /gene="vgrG"
                     /locus_tag="OHN28_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2299898.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSAYPSREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQGKVREMAP
                     AMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGVAI
                     ASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN"
     gene            4993..>5190
                     /locus_tag="OHN28_004632"
     CDS             4993..>5190
                     /locus_tag="OHN28_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299899.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVRNNNKSIMGEDYI"
BASE COUNT         1294 a         1438 c         1522 g          936 t
ORIGIN      
        1 ggatatcgac acgctctggc agtctctgct ggcacatctg gacaccttac cccgcccggt
       61 ccgcgaacgt cctcaacttt ctgaaccgct ggcagcgctt atccgacagg cgtggctgat
      121 agcgtcactg gaaggcgacg atccacaaat ccgcagccag catctgctga tggcgctgac
      181 agaaaaaccg atgctgcccg cctgtaatga cctgtgggta ttgctgagcc tgagccgtgt
      241 gcagcttgag cggctgcgtc ccctgctgga tgcgcagtcg gatgaatgtc cggcacgtca
      301 gccacaggtc accgaaccgc tgacatccgc gcaacccggg gcggcaacga cggacgcacc
      361 ggcaaaaacg ctgacgggga aacaggatga cgccctgctg gcggtgctta accgctttac
      421 cgaagacgtg acggaaaaag cccgcagcgg gcgaatcgac ccggtattcg ggcgcgacac
      481 ggaaattcgc cagatggtcg atatcctctc ccgtcgccgc aaaaacaacc cgattctggt
      541 gggagaaccg ggggtgggca aaaccgcgct ggtggaaggg ctggcgctgc gtatcaccga
      601 aggcaacgtg ccggacagcc tgaaaacggt gcatatccgc acactggacc tcggtctgtt
      661 acaggctggc gcgggcgtta aaggtgaatt tgaacagcgg ctgaaaaatg tcatcgatgc
      721 agtgcagaaa tcaccggagc cggtactgct gtttattgat gaagcccata ccattatcgg
      781 tgcgggtaat caggcaggcg gcgcggatgc ggcgaacctg ctgaaaccgg cgctggcaag
      841 gggcgaactg cgcaccatcg cggcgaccac gtggagcgaa tacaaacagt attttgagcg
      901 cgacgccgcg ctggaacgcc gcttccagat ggtgaaagtc gacgagccgg atgatgacac
      961 cgcctgcctg atgctgcggg gactcaaggc ccgctatgca cagcaccacg gcgtacatat
     1021 gctggacagc gccattcaga ccgccgtgcg cctgtcgcgc cgctatctga ccggacgcca
     1081 gttgccggac aaagcggttg atttgctgga taccgccggg gcaagagtcc gcatgagcct
     1141 tgacactctg ccggagccgt tgacgcagct tcatgcgcga ctggcggcgc tggatatcga
     1201 gcgggaggcg attgagcagg acagcgtgtt ttatcccgaa accagcccgg agcggctggc
     1261 ggaactgacc gatttgcgtg atgagctaca ggcagaagcc gggcatctgg aagcgcagta
     1321 tcagcaggaa aaggcactgg cgcagcagat tatgacgttg cgtcaggaag gaacagacag
     1381 ctctgaactg caacagcaac tgcggacgca tcagggcttt gcaccgctgc tggcgctgga
     1441 tgtggacgcc cgcgccgtcg ccacggtggt ggcggactgg accggcatcc cgctctcttc
     1501 cctgctcagg gacgagcaga gcgacctgct cagtatggaa cagagtcttg aaaaccgcgt
     1561 tgtcgggcaa agcccggcgc tctgcgccat cgcacagcgg ctgcgggcgg ctaaaaccgg
     1621 cctcacgccg gagaacggcc cacagggggt attcctgctg accggcccca gcggcaccgg
     1681 caaaaccgaa actgcgctca cactggccga caccctgttt ggtggtgaaa aatcccttat
     1741 caccattaat ctttcggaat accaggaacc gcataccgtt tcccagttga aaggctcgcc
     1801 tccaggctac gttggttacg gtcagggtgg cgtacttacc gaagccgttc gcaaacgccc
     1861 ttacagcgtg gtgctgctcg acgaagtgga aaaggcgcat cgcgacgtga tgaacctgtt
     1921 ctatcaggtg ttcgaccggg gctttatgcg cgacggcgaa gggcgggtaa tcgacttccg
     1981 caacaccgtc attctgatga ccgccaatct gggcagcgac cacatcatgc aactgctgga
     2041 ggaaaaaccg gacgccacgg acgcagacct gcatgaactg ctgtatcccc ttctgcgaga
     2101 ccattttcag cctgcactga tggcgcgttt tcagacggtg atttaccgcc cgctgggaca
     2161 ggaggcgatg cgcaccattg tggaaatgaa actggcgcag gtggtccgcc gtcttcacca
     2221 gcactacgga ctggaaacgg aaatcaacga cagcctgtat gaccagctta ccgccgcctg
     2281 cctgctgccg gataccgggg cgcgcaatat cgacagcctc ctgaaccagc aaatcctgcc
     2341 ggtcttaagc cagcagttgc tggcacagca ggccgtgcat cataagcctg cccgactgcg
     2401 gcttggctgg gatgaggagg acgggattgt actggaattt gatgagaaat aatggaggct
     2461 cttatgaacg tacagttctt cgaccacgca caccacaagc tcaaaattcg cggcctgaaa
     2521 agcccggtgg acgtgctgac ttttacaggc cacgaacagc tcagctcacc cttccgctac
     2581 gacattgagt ttaccagtac cgataaagcc atcgagccgg agtcggtact gatgcaggac
     2641 ggcgcattca gcctgagcgc cccgccggta caggggatgc cggtgcaggt ccccctgcgc
     2701 accctgcacg gcgtcattac cggctttaaa cacctctcct cgtcacagga cgaagcccgt
     2761 tacgaagtgc gccttgaacc ccgtatggcg ctgcttaccc gcagccgcca gaacgccatc
     2821 taccagaacc agacggtgcc gcaaatcgtc gaaaaaatcc tgcgcgagcg gcaccagatg
     2881 cgcggtcagg acttcgtgtt taacctcaaa agcgcatacc cgtcccgcga acaggtgatg
     2941 cagtacggtg aggacgacct gacgtttgtc tcacgcctgc tgtcggaagt gggtatctgg
     3001 ttccgctttg ccaccgacgc ccggctgaaa atcgaagttg tcgagtttta tgacgaccag
     3061 agcggctacg agcgcgggct gacactgccg cttcgccatc cttcagggct gtttgatggt
     3121 gaaacggaag cggtatgggg gctgaatacc gcgtacagcg tggtggaaaa aagcgtcacc
     3181 actcgggact acaactaccg caccgccaca gcagagatga tgacagagca gcatgacgcc
     3241 accgggggcg ataacaccac ctacggcgaa gcctaccact acgctgacaa cttcctgcaa
     3301 aaaggcgata aagaggcagc ggaaagcggc gcgttttatg cccgtatccg ccacgaacgc
     3361 tatttgaatg aacaggccat tctacagggc cagagcacca gttcactgct gatgccgggg
     3421 ctggagataa aagttcaggg tgacgacgcg ccagcggtgt tccgcaaggg cgtactgatt
     3481 accggcgtga cggccagcgc ggcgcgtgac cgcagctatg aactgacgtt taccgccatc
     3541 ccgtactcag aacgctatgg ctaccgcccg gcgcttatcc cctgccccgt gatggccggc
     3601 acgctcccgg cacgggtcac cagcacggtg aagaacgaca tctacgccca tatcgacaag
     3661 gacgggcgct accgggtcaa cctcgacttt gaccgcgata cgtggaaacc gggttatgaa
     3721 agcctgtggg tacgccagtc gcgcccgtat gcgggagaca cctacggcct gcatctgccg
     3781 ctgctggcgg gaacggaagt cagcatcgcg tttgaggaag gcaacccgga ccgaccttat
     3841 attgcaggcg taaaacacga ctcggcacac accgaccatg tgaccattca gaactacaag
     3901 cgcaacgtac tgcgcacccc ggcgaacaac aaaatccgcc tcgatgacga gcgcggcaaa
     3961 gagcatatca aggtcagtac cgagtatggc ggcaagagcc agctcaacct cggccatctg
     4021 gtggatgccg gaaaacagca gcgcggggaa ggctttgaac tgcgtaccga tctgtggggt
     4081 acagtacggg caaagaaagg catctttatc agctccgacg cgcaggacaa ggcgcagggt
     4141 aaggtgcgcg aaatggctcc ggcgatggca atactggatg gcgcacagtc acagatgaag
     4201 tcgctgtcca ctgatgcgca gacggcgaat gccgatccgg cagatctgtc atcacagata
     4261 gcgctgttgc agcagagtgt aaaagatctg acgcaggctg cgatcctgct aagcgcaccc
     4321 aaaggcgtgg cgatcgccag cggagagcac ctgcaactgg cggcgtcgaa aaacctgatc
     4381 gcgaacgcag gaaatcacgc tgatatcggt gtcgtgaaga atatgtttat cggcgtgggt
     4441 caggcgctga gcgtgtttgt acgcaaagcg ggtatcaaac tgtttgccaa taaaggggcg
     4501 atctcggtac aggcacagaa cgatctgatg gaactgttgg cgcagaaatc gatagagatc
     4561 accagtacgg aagatgagat taaaatcacc gcgaagaaga aaatcaccct taacggcggt
     4621 ggcagctaca tccggctgga tgcctgtggg atagaggccg gtacgccggg agaatataac
     4681 gttaaggcgg gatattacgg gcgtaagccg aaggcgaagc tgacgccgga gctgatggca
     4741 tttccggtga ttaagtctga ggattttaat cagagcttta ttttgctgga tgaaaatact
     4801 gggcaaccac ttattaattg gccctatgaa ctagaactgg aaagtgggct aaaaatgagt
     4861 gggattactg atgaaaatgg aaatacagaa ctaatatcct ctgataagga ggaggtcgtt
     4921 aatattagtg tgtttgaacc cgatgagttt ttagacgatg aaattaatta agacatatag
     4981 cgagaatata acatggcaga taaatccgaa aagaaaaaag aaaccacaaa taaatcagca
     5041 aaaaaaacac taacgaagaa gagcgaaaaa ttaactccag caaaagacac cgcaccaaaa
     5101 caagtgaatg tagatccagc tataagattg aaacaaattc gtcaacttgt ccgtaataat
     5161 aataaatcca tcatgggaga ggattatatt
//
LOCUS       DAJOTB010000237         5184 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJOTB010000237 DAJOTB010000000
VERSION     DAJOTB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.237"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(238..1260)
                     /locus_tag="OHN28_004633"
     CDS             complement(238..1260)
                     /locus_tag="OHN28_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HCQ2299900.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1245..2810)
                     /locus_tag="OHN28_004634"
     CDS             complement(1245..2810)
                     /locus_tag="OHN28_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2299901.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2885..3301)
                     /locus_tag="OHN28_004635"
     CDS             complement(2885..3301)
                     /locus_tag="OHN28_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HCQ2299902.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3298..3528)
                     /locus_tag="OHN28_004636"
     CDS             complement(3298..3528)
                     /locus_tag="OHN28_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2299903.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3909..>5184
                     /locus_tag="OHN28_004637"
     CDS             3909..>5184
                     /locus_tag="OHN28_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HCQ2299904.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKGMLYSI"
BASE COUNT         1261 a         1317 c         1302 g         1304 t
ORIGIN      
        1 cgcttcctgg ggggttaatg gctgatgacg ataaaatcat atatcgaatg atttatttct
       61 ctgcctgtaa atatcaggcc aggatttatt atgcgattaa tgataaatat catatcatct
      121 gcgggtttcc ctccacgcag gtccggtttc cggcgaaacc ggagcctcat ttttcggcct
      181 gaagttttgt cgttgactac tttttaccgg cttgtgccca gttgagaagc cgctgactta
      241 gtttctgaat ctccgaagat ggctttccgg aataaaatag atgttgcccc gtgcccgtga
      301 gcaggccacg tatagcctgt tacgggatga tggtggaagt gctgccagct tctgttggat
      361 gaggcgcatc cagtggttgg cccccatcac gatgcagacg tcctgaaagt ggtcgagtcc
      421 tttactgccc ccccagttca ttgagtggca tccgtaacgg tgatgttcgc gatagaagag
      481 cttgatcatc gtgttgtccg cgtgcagggc cgctgaacgc gcttcatccg tgacgatttc
      541 aatctgggtg gtatgtgttc catgagccga aatgcgaatg ttcagttgtc cggtaatgaa
      601 ctcacacact gtagctgaac agcgccatgt ccggctgagc gtctcacaat ccaccatgat
      661 acccgcagcc ctgaagcgag cctcgtatcg ggttatgtct tcatgcagcg tagcattgac
      721 gttaccgtcg cggcttgtgt caaaggtatg ctgataaaaa tccccgcagc acagcactga
      781 gatttccgcg cggcatagct ccagcagaaa attaaagtca tggccggcaa agtcctggac
      841 ttcatcaacg aacaactcat cgtagtaacg ggcaagtcgg gtacggatat ctggcagcaa
      901 cccccgggct gtcagcaggt gtgcgagccg ccggtgatac agccgtccgg cgggatcctg
      961 ataatgccgg atattcgtgc gcggtattct ggaagggggc tggttgaagc tcagaccacg
     1021 cgatgacagt tgctcctgca ggaaagggcg aaaacagaaa ccgtgcagaa actcaaacca
     1081 ggtcatcaca cggatcccgt acggtatgta gccaaagcgt ctgataatct gcgcccgcag
     1141 atgtgcttca ttatttacgg tgaaggtcag gatcagcgtc cgccggtcct ctcttaaccg
     1201 acgtatcagc agcgtggttt tacccgagcc ggcaaccgca aatatcactc tcttatccat
     1261 gccagtgcct cctggatata atcaggaacg gtcagctttt cggcgtgcaa ctgtaacagc
     1321 tggaatgctg cttctgcctt attggccagc atgtaatcct gaaccgtgag tgtccggcgg
     1381 gttccgcgga aaagcgcatc acacaggtcg gcattatcct gatacaggca gatttcaaat
     1441 gtggagcggc tgttgtcgtg gtcagcaaat acccgggagc gggaacaaag cacgtcagca
     1501 tagcgttcat cgcaattttg ctgatagttg ccgtcattgt cgcgaagggc cgcgacacga
     1561 ttttccagaa gacgggccag ttcaagataa cgacggaaac tcgttccgcc gatagcgatg
     1621 atgtgaaccc cgtcatcctc cggtgccctg ccatacaggc ggtgataaaa ggcctcgatc
     1681 agaataaact cagcatcccc ctccacgagc agcacgcgtc tggccagcgc aaattccagc
     1741 acgttattat ccggtgcttt catgaagaag gcggcagtct cagcggaaag ttcattcatc
     1801 agtaccggcc gggtagctcc gaggagaata gccttacgta ggtcgaggcg ggaggagata
     1861 tggctactgt gtgtggcaat aaacacctgc gtctgccgtt ccgtggcaag ctgattaacc
     1921 agcctcttca tactgacatg gctgagatga ttctccggct cctcaagcaa cagcgcgtgg
     1981 atctctcccc cctgctgctg atgccgctgc agcgcaaact ccgttttgat gaaacactgt
     2041 cttccttttc cgcggtgacg aatggaaata ccgtcctcag taatgtccag gttcgcctca
     2101 agacctgatt tagcgccgga acgcacgcca aactgatacg ttttcagcgt gtcgtttatg
     2161 gcggacagat gccgggcgca gaagtgcatt ttctgttgcc ggtaactgtt ttcaagccgg
     2221 taccgatcgg ccaccggcac attgacgctg taaactgtgc gcgtatactc ctgtgcggca
     2281 tgctcgttat caatgcgtgc actgtctaag aacagatggc gcagatagcg tctgaagcct
     2341 gcgaaatgac caccggagaa tgtgctgaac tgcacggaat agtattcata gggaaaatta
     2401 tcaggatcct gctgcaaaac atggtgtata tcctgaccgt attcctccat catgggagca
     2461 atgcgcattc tgagaccatc cgcatcgata cccgccagat tttgcctgcc gttcaggtca
     2521 ggctctcccc cattactcag gaaaacgtca gcggtcagca cgggtaactg atcggcacgg
     2581 cgctcacctt cctgaaaatg cctgactgct gactgtgaca ggagcgattc cacccccagc
     2641 gcctcaaccc gatgcctgct gtcactcagc acaaggtcaa gagccagcag aatagtgctt
     2701 ttcccggact cattgtcccc aaccagaatg ttacgatcgt tgctgaagcg caggtccagt
     2761 tccggaaact ttttgaagtt ttgcagcata agccgcgtga tcagtggcat ttttttattc
     2821 ctttatatac cgctgcctga aagagcggcc ggccgcgcct gaccgcctgt tttgttttac
     2881 agcgttatct tacccagtct tccagcacaa gacccggtac ccgctcaaac tctctcacat
     2941 tattcgtcac cagaatggca caggcagcga tggcgtgtcc ggctatcgcc gtgtcgttcg
     3001 gaccgatcgg cgtgccggcg agacgcagtg ccaccttaat ctccgtggtc gcatccaccg
     3061 cggcgcggtc ccacggcagg acggcatcga gcctctcaca gaacgcgtca accagttgca
     3121 cgtggcgtgg cgaggccttc ggaccggtgg caccgaagcg catctcggag taggtgatgg
     3181 ccgagaccac gatacggtga ccgcgcagca ccgactgctc caggtgctta agcaatgctt
     3241 caggttgctc gcgcatgatg aacgagcaga tgttcgtgtc gagcatatag atcttgttca
     3301 caggttaact cgtccttcgt cgctgacaac gtcctcgcgc tccgtcataa agtccggatc
     3361 ggccttttct agctccagga atgagcccca ggtcggccgg acggggcgca gaatgatgct
     3421 gtccccttcc cggacgatct ccagctcgct caccccctca aaatccagat cgcgtggaag
     3481 gcggatagca cggttgttgc catttttaaa aatggatacg gttctcatga ttctctcccg
     3541 ggtaagtgtg gatatgtgat gcatatccgg aatgatgttc attgcaccgg gtgtgtgcat
     3601 atgtttagta taggcagatg ttaggcatat gtatagattc agcatatgca gatggtgagg
     3661 atatgcgtat accatgaata tgcagatgtc aggcatatgg atatgttcag cctatgttat
     3721 tttctctctt tccccgtgca gatccggttt ctggtgaagt cggagcccac catctccgag
     3781 tatttgataa gccaattctt gtatcccttt gcctttatta tctttttggt tttcagaagt
     3841 tataatgcct gttaaatcaa caatgttgga agatcaacat gagaagacca tcccgaactg
     3901 aagcactgat gccagtactg agtgctaaca aagctcagga agaacagatt aacacctcct
     3961 atgaaccact gactccctca gtcattacag atgaaaaagc acaaagctat atcgaagctt
     4021 tgaattttgc ctgttctcgc ccggatatca gaaatattgc agtaacaggg ccgtacgggg
     4081 ccggtaaaag ttcggtatta ctgacatggg aaagggcgga ggataatgac tttcgggtga
     4141 tgaccgtatc actggctgac tttgaaatgc agcgggcata ccccggagat tcgctgtcag
     4201 ttgagggtaa gcccgactat gatgataacg aaaaaaaagc gggaaaagcc gaagagaaaa
     4261 caatcgagta cagcatcctg caacaactgc tctataaaga gaaaaaaagt gtgttaccct
     4321 attcccgtct ggagcgcatt tctgacgtaa gtgcgtgtca gatagccatg atgacggcca
     4381 gtctgttatt catactggct tcgacggcaa ctggtctgct ttttcttttc ccggattaca
     4441 tccgggaaaa gctctccctg tcgccagaac tgagtcagtt tctgctggag ttgcctgtac
     4501 tcgctaggtt tggtagcgcc gggatatttc tttttactgc tttattcttt gcactgaaga
     4561 aattgcaccg tacaggggta tttgatcggc gtgtaagtat tgataaagtt gacatactga
     4621 aaggtgccat tacaacacgc cctgcggccc cttctcttct caatgtgtac attgatgaaa
     4681 tcgtctactt tttcgaacag acacaataca atgtagtcat atttgaggat cttgaccgtc
     4741 ataatgacgg agccattttt atcaaactcc gggaaataaa tcagcttatc aataactgcc
     4801 tccccacaga caatcctgtc cgatttatct acgctgtcag ggataatctt ttcataacac
     4861 cagaatcacg gacaaaattt ttcgatttcg tcataccagt aatcccagta atggacagtg
     4921 agaatgcttc tgagcatttt ctaagtaaat ttacacttga tgaactcagg caggaaggat
     4981 tcaaagactg ccttgccaga ctggctcttt ttattcctga tatgcgggtt atgcataaca
     5041 ttgcgaatga atttcgtctt taccggaata ttgtgaataa tggaggagat ttaaagcgtc
     5101 ttatttcact gatcacctat aaaaaccttt atgctgaaga ctatcacagg atagatcaga
     5161 aaaaaggaat gctttatagc attg
//
LOCUS       DAJOTB010000238         5157 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJOTB010000238 DAJOTB010000000
VERSION     DAJOTB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.238"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            161..2401
                     /gene="fepA"
                     /locus_tag="OHN28_004638"
     CDS             161..2401
                     /gene="fepA"
                     /locus_tag="OHN28_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCQ2299905.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            2576..3235
                     /gene="entD"
                     /locus_tag="OHN28_004639"
     CDS             2576..3235
                     /gene="entD"
                     /locus_tag="OHN28_004639"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCQ2299906.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPTGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDAGFLRYQIRNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(3237..4172)
                     /locus_tag="OHN28_004640"
     CDS             complement(3237..4172)
                     /locus_tag="OHN28_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HCQ2299907.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGITLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(4165..4995)
                     /locus_tag="OHN28_004641"
     CDS             complement(4165..4995)
                     /locus_tag="OHN28_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2299908.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
BASE COUNT         1306 a         1360 c         1337 g         1154 t
ORIGIN      
        1 caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaatcagct gcactgcgtg
       61 tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgatga cgaccatgcc
      121 cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga agattcattc
      181 cctggccttg ttggtcaatc tggggattta tggggtagca caggcacaag agccgaccga
      241 tactcctgtt tcacatgacg atactattgt tgttaccgcc gccgagcaga acttgcaggc
      301 gcccggcgtt tcgaccatta ccgcagatga aatccgcaaa aacccggttg cccgcgatgt
      361 atcggagatc attcgtacca tgcctggcgt taacctgacc ggtaattcca ccagtggtca
      421 gcgtggtaat aaccgccaga ttgatattcg cggcatgggt ccggaaaaca cgctgatttt
      481 gattgacggc aagccggtaa gcagccgtaa ctctgtgcgt cagggctggc gtggcgagcg
      541 cgatacccgt ggtgatacct cctgggtgcc gcctgaaatg attgaacgta ttgaagttct
      601 acgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat
      661 taccaaaaaa ggcagcggtg agtggcacgg ctcctgggat gcttatttca atgcgccaga
      721 acataaagag gaaggtgcca ccaaacgcac caacttcagc ctgaccggtc cgctgggcga
      781 cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat
      841 caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg
      901 cgaaggggtg atcaataaag atattaacgg cgtggtgcgc tgggacttcg cgcctctgca
      961 atcactcgaa ctggaagcgg gctacagccg ccagggtaac ctgtatgcgg gtgatacgca
     1021 gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca accgcctgta
     1081 tcgccagaac tactcgctga cctggaacgg cggctgggat aacggcgtga ccaccagcaa
     1141 ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac
     1201 cgaagggaaa tttaacgaaa aagcggcaca ggattttgtc gatatcgatc ttgatgacgt
     1261 gatgctgcac agcgaagtta acctgccgat tgatttcctc gtaaaccaaa cgctgacgct
     1321 gggtacagag tggaatcagc aacggatgaa ggacttgagt tccaacaccc aggcactcac
     1381 cggaacgaat accggcggtg ctattgatgg tgtgagtgcc accgaccgta gcccatattc
     1441 aaaagcagaa attttctcgc tgtttgccga aaacaatatg gagctgactg acagcaccat
     1501 cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
     1561 gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca tcgcccgcgc
     1621 ctataaagcg ccgagcctgt accagactaa cccaaactac attctctaca gtaaaggtca
     1681 gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga
     1741 aaccagcatc aacaaggaga ttggtctgga gttcaaacgc gacggttggc tggcgggcgt
     1801 gacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtag ctgtagggaa
     1861 aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcgg tggttgaagg
     1921 tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacca ataacatcac
     1981 ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca tcccggagta
     2041 tacgttgaac tcaacgctga gctggcaggc acgggaagat ttgtcgatgc aaacgacctt
     2101 cacctggtac ggcaaacagc agccgaagaa gtacaactat aaaggtcagc cagcggttgg
     2161 accggaaacc aaagaaatca gtccgtacag cattgttggc ctgagcgcga cctgggatgt
     2221 gaccaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg
     2281 tgcgggtaat gcccagacca cgggcgatct ggcaggagcc aactatatcg caggtgccgg
     2341 ggcgtatacc tataacgagc cgggacgtac gtggtatatg agcattaata ctcacttctg
     2401 aagctaacgt cagattgttg acaaagtgcg cgtagttcat gccggatgcg gcgtgaacgc
     2461 cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccacg taggcctgat
     2521 aagcgtagcg catcaggcag ttttgcgttt gtcatcattc tctaatatgg ttgacatgaa
     2581 aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg agttcgatcc
     2641 ggcgagtttt cgcgagcagg atttactctg gctgccgcac tacgcacaac ttcaacacgc
     2701 tggacgtaaa cgtaaaacag agcatttagc cgggcggatc gctgcaattt atgcgctgcg
     2761 ggaatatggc tataaatgtg tgcctgcaat cggcgagcta cgccaacctg tgtggcctac
     2821 gggggtatac ggcagcatta gtcactgtgg gactacggca ttagccgtgg tatctcgtca
     2881 accgattggc attgatatcg aagagatttt ctctgtacaa accgcaagag aattgacaga
     2941 caacattatt acaccagcag aacacgagcg actcgcagaa tgcggtttaa ccttttctct
     3001 ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcatgtg aaagacaaac
     3061 tgatgcagga tttctgcgct accaaatcag aaattgtcag catcaacaga tgaatataag
     3121 cgggccgaat ggcgaccata tcgtgcagtg gttagtaagg ggtaataatg ttattacctt
     3181 gtgcctggca gataaacacc cgcacactgc agcatgcggg ttatctggcg ggtaattcaa
     3241 agcatttccc gtgcctgagc tgcaattccg gcagcatcaa atccatgatg ctgacgcaac
     3301 cacccacgat ccgccgctgc ggcatattct ccatccataa tgcctgcgcg ttttaatgtg
     3361 atccccgttc cggcctctgc caacacttcc gcgaccaggc taccaaggcc gccattgacg
     3421 ttatgctctt caaccgtcat cactgctttg catcctttta acgctgccag caatgctttg
     3481 gtgtcgcatg ggcgaatcga cggtacgctg acaactgtag ccgcgatacc agactgcgct
     3541 aaaagttctg cggcatcgac cacttcatga acggtagagc ctgtggctac cagcgccagt
     3601 tcgctgccct cgcgcaaagt caccacggca cctggtacaa atcggtagtc ctcatcgtgg
     3661 atttccggca gggctttacc gtccatacgg atataaacag gcccctgata gcgaagagca
     3721 taatcaatca cctgacggca ctcccgcggt gaagacgggg caaagatttg aatattgccg
     3781 aaaccacgca taaccgcaat gtcatcaatc gcatgatgcg tgctcgccag cggtccataa
     3841 ctcgccccgg cattcagacc gaacaatttt acgttggtaa agttgtagca gacatcaacc
     3901 ttgatttgct cgttagcgcg agcgataaga aacggcgcgg cattgcaggt aacagccacc
     3961 ttgccgccca gcgccagccc ggcagctgta ccaaccatgc tctgttcggc aatacccaca
     4021 ttcaccagtc ggtcagggaa ttgcttaata aagggagcaa tttttgccgt agacgttgaa
     4081 tcagccacca caggcaccaa atcgatcccc tcatttacgg catcgataaa cgcctgaacc
     4141 atcacatttg ccagatgttc actgttacgc atctttgagt tcctccagtg cctgttcaat
     4201 ttcatcgcct ttcggcaccc ggtgatgcca ttccgcacgc ccttgtataa aggaaatgcc
     4261 tgccccttta gtggtgtggg caatcaccac attcggtttg ccttcctgtt taagctcttc
     4321 aagcgtcgcg acaaccgaag ccatatcatt gccctgacat tcagtaacct gcataccgaa
     4381 tgccagccat ttctctgcca gaggatcggt gttcatgatt tcgcgcgttg gcccggctaa
     4441 ttgcagatta tttttgtcat taataatgac caggttatca agcccgtaat gcgccgccgc
     4501 cagtgcagct tcccagttac tgccctcagc cagctcacca tcgccagtaa ttaagaagat
     4561 acgacgacgg ctttgacttt ttttcgccgc cagcgcaagt cctacagcca cagggaaacc
     4621 atgccccagt gctccggtat tcagttcaat acctggcgtt ttttgccgaa ccggatggcc
     4681 gggtaagtgt gaatttggct gctgataagt ggaaagccat tccagtggga agaatcctgc
     4741 ttccgccagc acgcagtagt aacatccaac agcatggcct tttgactgaa tgtatatatc
     4801 ccggtcatcg tcgtgaaggc gatccggtgc gacattgaga atacgaaaat agagtgcggt
     4861 cagtaattca acctgagaga gatcggcccc ggtgtgtcca cctgccgggc tattggcatt
     4921 taagcgcacg atatggcggc gtaccgcccg cgctttacgc tcaagggtct ggatatcata
     4981 gctataggga ttcatttaat acctctgaat ttattttcaa catggaatat ttattccacc
     5041 ggggactaaa aaaaccagaa atcccagggc gttgcgcggc aagacttacg ctgctgcatc
     5101 atactcagga acattctgcc tccttacacc gtcagttaca tgtccgggta atcatca
//
LOCUS       DAJOTB010000239         5096 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJOTB010000239 DAJOTB010000000
VERSION     DAJOTB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.239"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..214
                     /locus_tag="OHN28_004642"
     CDS             <1..214
                     /locus_tag="OHN28_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299909.1"
                     /translation="LIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDN
                     WETAGAIAGGAAAVAGLTMGIIALSK"
     gene            645..1670
                     /locus_tag="OHN28_004643"
     CDS             645..1670
                     /locus_tag="OHN28_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2299910.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1738..2919
                     /locus_tag="OHN28_004644"
     CDS             1738..2919
                     /locus_tag="OHN28_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2299911.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            2929..4032
                     /locus_tag="OHN28_004645"
     CDS             2929..4032
                     /locus_tag="OHN28_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2299912.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            4040..4798
                     /locus_tag="OHN28_004646"
     CDS             4040..4798
                     /locus_tag="OHN28_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCQ2299913.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(4861..4976)
                     /gene="rrf"
                     /locus_tag="OHN28_004647"
     rRNA            complement(4861..4976)
                     /gene="rrf"
                     /locus_tag="OHN28_004647"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5016..5091)
                     /locus_tag="OHN28_004648"
     tRNA            complement(5016..5091)
                     /locus_tag="OHN28_004648"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5056..5058),aa:Thr,seq:ggt)
BASE COUNT         1175 a         1212 c         1305 g         1404 t
ORIGIN      
        1 attaattcct gtggcattgc tcaccgcatt gctggcgggc tgtgctcacg attcgccctg
       61 cgttccggtt tatgacgatc agggtcgact ggttcacact aatacctgta tgaaaggcac
      121 gactcaggat aactgggaaa cggctggggc tattgccggc ggggcagcag ctgttgctgg
      181 cctgacgatg gggatcatcg ctctgtcgaa ataactaaac agggcgcgga gctccgcgct
      241 ctttttaacg atacagtatc tttccataag ataaaatatt gctatattct gctgggtaat
      301 tccctgctga tttagcatgt tatatcacgt tatttcgctg ctttcgaaat aacgcatcaa
      361 tttaactttc acacttccca ccagaaatat actcacgcta acaatcatta ttctgcttag
      421 taaaaaacgc gtaaatattt tgcgccaaaa tgtggcgcat gtttcatttt cgcaccatta
      481 cgaggcgctg tttttattat ttcctgtcta cactcagctt attgcgacgc gaaatcgtgc
      541 gcaaaagctg gcactacttt tgcttataag aggatggcca cagacaggta aaagacgttt
      601 cccaaacgtc ctataacgat aattttcgcc tcacaggaag cattatgaaa aagatgatga
      661 tagccacact ggctgccgcc agcgtgctgc ttgccgttgc aaatcaggcg catgctggcg
      721 cgacgcttga tgccgttcag aaaaaaggtt ttgtgcaatg cgggatcagt gatggattac
      781 ctgggttctc ttatgccgat gctgacggta agttttcagg tattgatgta gatgtttgtc
      841 gtggtgttgc cgctgctgta ttcggtgacg atacgaaagt gaaatatacc ccgctcactg
      901 caaaagaacg cttcaccgct ttacagtcag gggaggtgga tttgctctcc cgtaatacga
      961 cctggacttc atctcgcgat gccgggatgg gaatggcatt tactggcgtc acttattacg
     1021 acggcattgg cttcctgacg cacgataaag cggggctaaa aagcgcgaaa gaactggatg
     1081 gcgctaccgt ctgtattcag gcggggactg ataccgaact caacgtcgcc gattacttca
     1141 aggcaaataa tatgaagtac acgccagtaa ccttcgatcg ctcagacgaa tcagcgaagg
     1201 ctctggaatc tggccgctgt gatactctgg cctcggatca atcacaactg tatgccctac
     1261 gcatcaaatt aagcaaccca actgaatgga ttgtcttacc ggaagttatc tctaaagaac
     1321 cacttgggcc ggtagttcgt cgtggcgatg atgaatggtt ctcgattgta cgctggacgc
     1381 ttttcgccat gctgaatgct gaagagatgg gcatcaattc ccagaatgtc gatgaaaaag
     1441 cggctaatcc agcaacgcct gatatggcac atctgctggg taaagaaggc gattacggca
     1501 aggatctgaa gctggataat aaatgggcct ataacatcat caaacaggtg ggtaactact
     1561 cggaaatttt cgagcgtaac gtaggttcag aaagcccgct gaaaattaaa cgtgggcaaa
     1621 ataatctctg gaataacggc ggtattcagt acgcgccgcc cgtgcgttaa gtcggtgtga
     1681 ggtaacgagc gttgctacgg cggcgctctt ttcagcgtta tggttttcga ggtttctatg
     1741 ccccatcgcc gctcaaccgt taaaggctca ctctcttttg ccaaccctac ggttcgcgcc
     1801 tggttattcc agatccttgc cgttgttgct gctgtcagca ttgttggttg gttatttcac
     1861 aacactgtaa cgaatctcag taatcgtggc ataacatcag gatttgcttt tcttgaccgc
     1921 ggggccggtt ttggcatcgt tcagcatttg attgattatc agcaaggcga cacttacgga
     1981 cgcattttta ttgtcggcct actcaatacg ctactggttt ctgcattgtg tattgtgttc
     2041 gcttctgttt tgggcttctt tatcggtctg gcgagacttt cggataactg gctgctacga
     2101 aagctttcca caatttatat tgagatcttc cgtaatattc ccccgctgct gcaaatcttc
     2161 ttctggtact ttgccgtgtt gcgcaatttg cccggaccac gacaggcagt gagcgcgttt
     2221 gacctggcct ttttgagcaa tcgtgggctt tatattccgt cgccgcagct gggagacgga
     2281 tttattgcgt ttatcctggc tgttgttatg gctatagtcc tttctgttgg gctattccgc
     2341 tttaataaaa cataccagat aaagaccgga caactgcgcc gcacctggcc attcgccgct
     2401 gtgttgatca ttggtttgcc tttactggcg caatggcttt ttggcgcagc actacactgg
     2461 gatgtcccag cccttcgagg ctttaatttc cgcggcggga tggttttaat tcctgaactg
     2521 gcagccttaa cgctagcact ttcgatttat acctctgcat ttatcgccga gattatccgc
     2581 gcagggatcc aggcagtgcc ttatggtcaa catgaagcgg ctcgatcact gggattaccc
     2641 aacccggtta cgctacgcca ggtcattatt ccccaggcat tgcgggtgat tattccaccg
     2701 ttaaccagcc agtatctcaa catcgtcaag aactcctctc ttgccgccgc tattggctat
     2761 cccgatatgg tttcgctgtt tgctggcacc gtgctgaacc aaacggggca agccatcgag
     2821 acgatagcca tgaccatgtc ggtctatctg attatcagcc tgactatctc gctgctgatg
     2881 aatatctata accgccgcat cgcgatcgtt gaacgctaag gagtcatgat gacaaaagta
     2941 ttgctgtctc atcccccgcg cccggcgagc cataactcaa gccgcgcgat ggtgtgggtg
     3001 cgaaaaaatc tgttctccag ctggagcaat agcctgctga ctattggctg catatggttg
     3061 atgtgggaac tgatcccacc gttgctgaac tgggcatttt tgcaggctaa ctgggttggc
     3121 tcaacgcgtg ccgactgcac aaaagccggt gcctgttggg tcttcatcca cgaacgattt
     3181 ggtcagttta tgtatggact ttacccacac gaccaacgct ggcgaattaa cctggcatta
     3241 ctgattggcc ttgtgtctat cgcaccaatg ttctggaaaa tacttccgca tcgcggtcgc
     3301 tatattgcgg tctgggcggt gatttaccca ctggttgtct ggtggctgat gtatggtggg
     3361 tttcttggtc ttgatcgggt tgaaacccgg caatggggcg ggttgacgct aactttaatt
     3421 attgcatcag ttgggattgc gggggcgcta ccgtggggga tcttactggc gttaggtcgc
     3481 cgctcccata tgccgattgt gcgtatctta tcggtcattt ttatcgagtt ctggcgcggc
     3541 gtaccgctga ttaccgtatt gtttatgtct tcggtcatgc tgccgttgtt tatggcagaa
     3601 ggcaccagta tcgataaact gatccgcgcg ctggttggcg tgatcctgtt tcagtcagca
     3661 tatgttgcgg aagtcgtgcg aggcggatta caggcgctgc ctaaagggca atatgaagcg
     3721 gcagagtcgc tggcattggg ttattggaaa actcaggggc ttgttattct gccccaggcg
     3781 ttgaagctgg taattcctgg gctggtaaat accatcatcg cactcttcaa agataccagt
     3841 ctggtgatca tcatcgggtt gttcgatctt ttcagtagcg ttcagcaggc aaccgttgat
     3901 cccgcgtggt tgggtatgtc gacggaaggg tatgttttcg ccgcgctgat ctactggatc
     3961 ttctgtttca gcatgtcacg ttatagccaa catctggaaa aacgttttaa caccgggcgt
     4021 acaccgcatt gaggacacta tgagccaaat tttactgcaa cctgctaacg cgatgattac
     4081 gctggaaaac gtcaataaat ggtatggaca attccatgtt ttgaaaaata taaatttaac
     4141 cgtgcaaccg ggcgaacgga tcgttctatg tggcccttca ggttccggta aatcaacaac
     4201 cattcgttgt attaatcatc tggaagaaca tcaacaggga cggatcgtgg tagatggcat
     4261 cgaacttaat gaagatatcc gcaatattga gcgcgtcagg caggaagtgg gaatggtctt
     4321 tcagcatttc aatctcttcc ctcatctgac cgtgttacag aactgtaccc tggcaccgat
     4381 ttgggtacgc aagatgccaa agaaagaggc tgaagctctg gcgatgcatt accttgagcg
     4441 ggtgagaatt gccgaacatg cccagaagtt tcccggacag atttctggtg gtcagcagca
     4501 acgtgttgcc atagcacgtt cgctgtgtat gaagccgaaa attatgttgt ttgatgagcc
     4561 aacgtcggcg ctcgatcctg agatggtgaa agaggtgctg gatacgatga ttgggctggc
     4621 gcagtcgggt atgacaatgt tgtgtgtaac acatgagatg gggtttgcac gaaccgtcgc
     4681 tgaccgggtg atttttatgg atcgtgggga aatagtggag caagctgcac ctgatgaatt
     4741 ttttgcgcat cctaaatcag agcgtacgag ggcattttta tcgcaggtaa tccattaatt
     4801 gaatattagt tcgaaaagca aaaaggccat cctttcggat ggcctttcgc ctgatttgat
     4861 gtctggcagt tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt
     4921 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccagacaaa
     4981 ttcttttcta atctgccgaa ctttaaccta aaaagtggtg ctgataccca gagtcgaact
     5041 ggggacctca cccttaccaa gggtgcgctc taccaactga gccatatcag cacgct
//
LOCUS       DAJOTB010000240         5029 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJOTB010000240 DAJOTB010000000
VERSION     DAJOTB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.240"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..347
                     /locus_tag="OHN28_004649"
                     /pseudo
     CDS             1..347
                     /locus_tag="OHN28_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            537..1103
                     /gene="slp"
                     /locus_tag="OHN28_004650"
     CDS             537..1103
                     /gene="slp"
                     /locus_tag="OHN28_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCQ2299914.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1256..1786
                     /locus_tag="OHN28_004651"
     CDS             1256..1786
                     /locus_tag="OHN28_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2299915.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(1843..2871)
                     /gene="chuS"
                     /locus_tag="OHN28_004652"
     CDS             complement(1843..2871)
                     /gene="chuS"
                     /locus_tag="OHN28_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HCQ2299916.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2920..4902)
                     /gene="chuA"
                     /locus_tag="OHN28_004653"
                     /gene_synonym="shuA"
     CDS             complement(2920..4902)
                     /gene="chuA"
                     /locus_tag="OHN28_004653"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HCQ2299917.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1399 a         1223 c         1095 g         1312 t
ORIGIN      
        1 acatataacg gtgatattta taactgcaca acaaataccg ataatgttaa aacgccagac
       61 acaccgaagt ggccttgtga taaccgggaa gaacaacaac ccattaattc cccattctct
      121 ggagaaggat acacctctaa acaatttgat ttagcgcaac aacaacgcca acaaattaat
      181 gcgtgccata cgaacaccac atatacaaat gcagactact caaagttgag gctcaacttg
      241 taagtcttat taataacatt gaaacgataa gctcgacacc acttccgcaa aagacacaat
      301 caatacttaa tcagataaat aacattagac acgaaaagat taaaaacctc ctaatataaa
      361 ttatggaaca atggatactc atgccacgcc caaaaattaa ctgagctcaa ctaaacagaa
      421 gagatataaa catcagacag gtttacgtta ctatcaggca tatcgcctca gaatcagatg
      481 aaaactataa agaaatatct attatggttt taatatttgt tgataaggat agtaacatga
      541 acatgacaaa aggtgcactc atcctcagcc tttcattttt gcttgccgca tgtagttcaa
      601 ttccgcaaaa tatcaaaggc aataaccaac ctgatattca aaaaagtttt gttgctgttc
      661 ataaccagcc ggggttatat gttggtcaac aagcgcgctt tggtgggaag gttatcaacg
      721 ttatcaatgg caaaacggat acgttgttag aaatcgctgt attaccgttg gatagctatg
      781 cgaagcctga tattgaagcc aactatcagg gccgactgct cgccagacaa agcggcttcc
      841 ttgatccagt gaactatcgt aatcactttg ttaccatcct cggtaccatt cagggtgaac
      901 aacctggctt tatcaataaa gtcccgtata acttcctcga agtgaatatg cagggcatcc
      961 aggtgtggca tttgagagaa gtggttaata ccacctataa cctgtgggat tacggctatg
     1021 gtgcattctg gccggaaccg ggctgggggg ctccttacta caccaatgcg gtgagtcagg
     1081 taacacctga gctggtcaaa taacaccacc gaaagatgca gatgctttct cagcatctgc
     1141 atcatgcatt acatcaaatt aatacacagt aagctaacta ttattataag ccctgtcctg
     1201 ttaattacct ttggcaaact gattataaag ttaatgtccg taccaggagt cggttatgtt
     1261 tcttataatt accagggata cgatgttttt caccgcgatg aaaaacattc tgagtaaagg
     1321 taatgtcgtt catatacaga acgaagaaga gatcgacgta atgttgcatc agaatgcctt
     1381 cgtcattatt gatacattaa tgaataatgt atttcattct aattttctca ctcaaattga
     1441 acgattaaaa cctgtccatg taattatttt ctcccccttt aatattaaac gctgcctggg
     1501 gaaagtaccg gtgacctttg ttccgcgaac tatcgccatc atcgactttg tcgcactcat
     1561 caatggcagt tactgctctg tgcctgaagc ggatgtgtcc ctctcgcgca agcagcatca
     1621 ggttctgagc tgtattgcga atcaaatgac aacggaagat attctggaga aactgaaaat
     1681 atcgctaaaa acgttctact gccataaaca caatatcatg atgatcctca atcttaagcg
     1741 gatcaatgag ctggtacgcc atcagcatat taattatctg gtgtgaacga ttgatcaata
     1801 taaagaggcc cagcaacagc cagacctccc gttaattata cgttatgcag taacgccttc
     1861 cggtatcaac gaagcaattt gcttacgcca ttgcgcttgc tcctgttcac cttctgtacg
     1921 ttgaccataa agttgcgcta tctgcgtacc atcatgggca aacaattcca gactggttac
     1981 gtgaccatcg ctggtcggtt tacgggtaac ccaggcttca gcaatgctct cttctaatag
     2041 atgaagagta aacgtcgggt tgaaaatatt cagccaacct ttcattggca ccactttttc
     2101 taccacaccg gtgaaaatct gtacgcagcc acggttgcca acaaacacca tgatttcatt
     2161 gccatcctgc tgtgcagatt caagaatttg cgccaacgca ctgttggata ctttgcaggc
     2221 caaatcgtct gccaccagat tgaacgcctg ttggcgcgtc agattgtgac gcttgagcaa
     2281 cgtaaaaaac tgatgaacgt cggtcatcgc ccgccactct tgctcgacca cactggcatc
     2341 ggctcgcgtt tgaacaactg gcgcatcaac ggcttttaac tcaagcggcg tattctcatc
     2401 ggtgataaac cgtgccagaa gctcactcca tgccgccata tcggtattgt cggtggcata
     2461 aacttttagt aatgcatcgc cctgatgatc aaagaactga atactctggc gttcgccacg
     2521 ggccgtgttt tctttgatgt ggaaaacact ggcccattga ttgagaaaca gacgtaaatc
     2581 cagcgcgcgc ggattgagga tcaatccggc atgtccgttc aaatgctggt ttgtgaacgc
     2641 accaacttgc tcatggactg catattcatt acggcaaata catttggttt cgccaacact
     2701 ttcgagcgcc gccagaattt cacgaatatc gccgcgcatc cgccacgcgt cgtgcgtgac
     2761 gcgcgcaaat gccagttctg cttcgctgat attcattaaa cctgcgatgt cacgcgcgta
     2821 ctttccggga ttttgttctt ttaactcaag ccagcgtgtg tagtggttca ttgtctctct
     2881 tccttccaga taaatgcccc gaaatatcgg ggcagatgat taccattgat aactcacgaa
     2941 aatttttccg ttacgaccat cctgtgggat gccttgcggc gaccagtact ctttatcgaa
     3001 ggcgttgccc aataccagag tagtggtcat gcctttgagc gcctgttgtc cttgataact
     3061 gacgtagaaa tcattcacac catagccagg ttgtttgctg tagctgctgc tgatatgtgt
     3121 tgagcgatcg gcaaacgtac cgacccaacc aacagagaag ccactgtgag cgatcggaat
     3181 attcagggta ctggtaacgg tatccgggtt aatgctggag atatattccc cggtatcggt
     3241 gtctttgccg cgggtacggt tataggccac atcaaggcta aacagatcag tggtatattt
     3301 cgtcatcaca tcccagcccc agattttggc gttcgggacg ttatacgaca tagttgtcgc
     3361 cgccgcgaaa tcgacggtcg tggagatata atctttcgct ttggtatcaa agtagctggc
     3421 tttaaattcc agagcatcat tggacaacat caggtcatca aaacgtagcc caaaaccgta
     3481 ctcctgagtt tcgttagttt ccggacgtaa gttcgggttt ggcacccaat agttggtata
     3541 gaagcgacca atcgagaagt gtttagaatc attatacatt tcgcccatcg tcggggcgcg
     3601 gaatgcctga gcatatgagc caaataacat cagccagttg gtcgggttga tagtcatccc
     3661 cgcacgagat gaccatttgt cggcatcaac atctttgtag ccgtcgctgc taccgcgata
     3721 actgtcatag cgggttccgc caagcagggt aatcggcaga tcgcgtaagg tgatctcatc
     3781 ttgtagccag ccagagctaa aatcgatttt tgcttgcggg aagcccgtcg tcgcgccacc
     3841 cggatgttgt tcctgacgat aatactcacc gccatatgtc agtaagtgag aagcgaaact
     3901 gtcggcaaat agagtggaac ggttctccag ccttgctcct tttgttatct gttcacgata
     3961 ctcgcctgaa ctccccgtgt tttgcgcatt aatacggact tccgaccagt atatttttgc
     4021 atctgcattt aaccagtcgt taccctgcgg ggcgagttta taagaaagct gcgcatcgcg
     4081 ttgaattgtt gaacgatcga ccatcgggtt gctgctatca gaagcttcaa cagtctgcgg
     4141 attttttggt tcacgcgcgt cgttgttgta gtaacgcact aaaccgctca gagactgggc
     4201 tgaatcaatt tgccaggtcc ctttcgccag catgttatta atggattcgt cattcggcgc
     4261 ggtttcacca ttgctctggc gtaaatcacc ccgatcgcga ctggaccagg ccacaatacc
     4321 atccagattt tcagttcgcc caaacgcgct cgcgcctaat cccaggctat ggtcccccgt
     4381 gccgccagta ccaaagacac gaaaaccact gctttgtcct tcctgcaata aatcttttgc
     4441 atcgaccgta tcgtaggaga tcactccacc cagcgcgcca ctgccataca gtaatgctga
     4501 aggtccgcga acaatctcaa cacgcttgat cagcgccgga tcgagaaaag tgccattcag
     4561 gtgtccggta tccgttccct ggcgaacacc atcgacaaga accagcacgc cgcgatgatc
     4621 atagccacgc atatttacat cctgaccgtt ggttcgtccg gtaccatcaa gagtaattcc
     4681 aggaacatga cgcagcaaat cagtggctga agtagcagtt tgattttcag gagcggaagt
     4741 gtcgataacg ctgaccatca taggcgcttc gaaggaacta cgtgcattcc ccgttgccgt
     4801 aacggtcatg gtttcagtag caaaagcaaa cgttggcaag gtggcagaaa cagccaaagc
     4861 caacaaactc aaacgcaacg aagtaaattg cggacgtgac atagcgattc tccatgagga
     4921 aaataaaaaa tgctggctgc ctgggttaat accggggtgg ctggcgaaaa ataaatttat
     4981 ttacaattca aatgattaga tatcaccacg ggaagctatt agcgtatat
//
LOCUS       DAJOTB010000241         5027 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJOTB010000241 DAJOTB010000000
VERSION     DAJOTB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.241"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            287..586
                     /gene="daaA"
                     /locus_tag="OHN28_004654"
                     /gene_synonym="afaA"
     CDS             287..586
                     /gene="daaA"
                     /locus_tag="OHN28_004654"
                     /gene_synonym="afaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaA"
                     /protein_id="HCQ2299918.1"
                     /translation="MNSSDLYLSKKHGCTLSPGKMSESQFWLLAEISPVHSEKVLNAL
                     RDFLVLGYTRREACERHEVSQGYFSGALGRIQRTHQAVNRLVPFYISEAGIPYTG"
     gene            594..1136
                     /locus_tag="OHN28_004655"
     CDS             594..1136
                     /locus_tag="OHN28_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2299919.1"
                     /translation="MKNLSLSVLLLLAGGGVSHAAVQKTVFSADVVASACHVVVDADS
                     TGNSGRLTFGTYRKSTGAPVPPRDFTVRLYESGATVQGCSAFKAGQVATLDFGNPGQL
                     DAGGVVTRGAGDGIRVDVRAVDAQADYRGRLTQDSHSVNYPVEFAAKGQFRFRAQPVF
                     PVNVKAGEYTGALTFVVTYQ"
     gene            1193..3595
                     /gene="faeD"
                     /locus_tag="OHN28_004656"
     CDS             1193..3595
                     /gene="faeD"
                     /locus_tag="OHN28_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HCQ2299920.1"
                     /translation="MFFRRAALALAVSAAFASATGIAGEKLDMSFIQGGAGVNPEVWA
                     ALNGSYAPGRYLVDLSLNGKEAGKQILDVTSQDSNELCLTEAWLTKAGIYVSADYFRE
                     GYDATRQCWVLTKAPSVKVDFDVSTQSLTLSIPQKGLVKMPENVDWDYGTSAFRVNYN
                     ANANTGRNNTSAFGSADLKANIGRWVVSSSATASGGDSGDNSTTINMFTATRAIRALS
                     ADLAVGKTSTGDSMLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIANGPSRVTLTQNG
                     RLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQVQNFPLSVMAGQLSPGQHEF
                     SVAAGLPDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNQDWQGVSAGAVLGLSYLGAV
                     SADGAYATAKYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEEYEDMSSFDPTEL
                     WSQSNHGRRTKDEWNAGVSQPVGGLFSLSVSGWERSYYPASMTGSYRYSDDNGKDTGI
                     TGTLSTQIKGVSLNLGWSGSRNTRGENNWSASASVSVPFTLFDRRYSSSTSVSTSKDG
                     GTGFSTGVSGSLNDRFSYGLGGGRDSDGGASSYLNASYSGDRAYLSGALNHSQSGGTS
                     GSVSVSGSVLAVPAAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDGQTDSDGNLVVPL
                     NSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVKRYLLQVKQRDGE
                     FVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAAKLQDTEKLQEIT
                     CE"
     gene            3588..4388
                     /gene="faeE"
                     /locus_tag="OHN28_004657"
     CDS             3588..4388
                     /gene="faeE"
                     /locus_tag="OHN28_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HCQ2299921.1"
                     /translation="MSKRNAVTTFFTNRVTKALGMTLALMMTCQSAMASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFRVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     LSLQSRPDGRTMLVNTTPYIYAIGSLLDGNGKKIATDNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLSDYGELQTWTINQKKTSASSGEKVSDSSVNLPDKADKADKK"
     gene            4423..4914
                     /gene="faeF"
                     /locus_tag="OHN28_004658"
     CDS             4423..4914
                     /gene="faeF"
                     /locus_tag="OHN28_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HCQ2299922.1"
                     /translation="MKKTMMAATLVLSALSIQSALAAEYSEKTQYLGVVNGQVSGNSV
                     VKVTRTPTDPVLYRSGSNSPLPAELIIRHAESRPASGGLANITVKQTLPDNGEARITL
                     KTALMVDGKRVTLSARQQGEDVVITVPEAQKLVELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
BASE COUNT         1160 a         1163 c         1607 g         1097 t
ORIGIN      
        1 aaacctgtcc aggaggcaat attggtgttt agaaaaacga cgatcttttt atctgttttt
       61 tgttttgttt ttggtttttc atcctaaggt gctggggttt tgatggtatt ttgtgccatt
      121 aagtggttgt tgtctggtta taaagatcgt gaaattgatg tttttcgcta taaaacaccg
      181 tgataatctt cgacgcagaa aacaggacgg cagcagagtg aatacgtttt tgttagctgg
      241 atttatgtcg tctttttgtc tggttatgtt tctggaatag atataaatga acagcagtga
      301 cctgtatctg agcaagaagc acgggtgtac tttgtcgccc ggaaaaatgt cagagagcca
      361 gttctggtta ttagctgaaa tatctccggt tcacagtgaa aaggttctta atgcgctgag
      421 ggattttttg gttctgggtt atacccgaag ggaggcctgt gaacgacatg aggtttctca
      481 gggatatttc tctggtgctc tcgggcgtat tcagcgtacc catcaggcgg taaacaggct
      541 ggttcctttt tatatatcag aagccggtat tccatatacg ggataacagt tgcatgaaaa
      601 atttatcgct ttcagtactt ctgcttctgg cagggggcgg ggtttctcac gccgctgttc
      661 agaaaaccgt attcagtgct gacgtggtgg cttcggcctg tcatgtggtg gtggatgcgg
      721 acagcaccgg caacagtggc cggctgacat tcggaactta ccgcaagtcc acaggggcgc
      781 ctgtgccgcc gcgtgatttc actgtgcgtc tgtatgagtc cggtgccacg gttcagggct
      841 gctcagcctt taaggcaggg caggtcgcca ccctggattt tggtaacccg ggccagctgg
      901 acgccggggg cgtggtcacc cgcggtgccg gtgatggtat tcgcgtggat gtgcgggcgg
      961 tggatgcgca ggcagattat cgcggacgtc tgactcagga cagtcattcg gtgaattacc
     1021 cggtggagtt tgctgctaaa ggacagttcc gttttcgtgc gcagccggtg tttccggtaa
     1081 acgtgaaggc gggggaatac acgggggcgc tgacctttgt ggtcacgtat cagtaacgga
     1141 gaaaaggatt gtgtgtgcag gaaagaaggc agtagcagag ccggtttcat ccgtgttttt
     1201 ccgtcgggcc gcacttgcgc ttgccgtatc agctgctttt gccagtgcca caggtattgc
     1261 aggtgaaaaa ctggatatgt cctttatcca gggtggtgcc ggggttaatc cggaagtctg
     1321 ggcagccctg aacggcagct atgcgccggg gcgttatctg gttgacctgt ccctgaacgg
     1381 gaaggaggcc gggaaacaga tactggatgt gacatcacag gacagtaatg aactgtgtct
     1441 gacagaggca tggctgacga aggccgggat ttacgtcagt gcagattact ttcgtgaggg
     1501 atatgacgcc acacgacagt gctgggtgct gacaaaagcc ccgtcagtga aggtggattt
     1561 tgatgtttcc acccagagtc tgacgctgtc cattccccag aaggggctgg tgaagatgcc
     1621 ggagaatgtg gactgggatt acgggaccag tgcatttcgc gtgaactata acgcgaacgc
     1681 caacaccggc cgtaataaca cctcggcctt tggctcagcg gacctgaaag ccaatatcgg
     1741 gcgctgggtg gtgagttctt ctgccacggc cagcggcggt gacagcgggg ataactccac
     1801 cacgataaac atgtttacgg ccacccgggc catccgcgca ctgagtgcgg acctggcggt
     1861 cgggaaaaca tccaccggtg acagcatgct gggcagtacg ggaacgtacg gggtgtcact
     1921 gagccggaac aacagcatga agccgggcaa cctggggtac accccggtgt tcagcggcat
     1981 tgcgaacggg ccgtcgaggg tgacgctgac acagaacggg cggttgctgc attcggagat
     2041 ggtgccggcg ggtccgttct ccatcacgga tgtgccgctg tacaccagtg gtgatgtgac
     2101 catgaaaatc accggtgaag acgggcgcga gcaggtacag aacttcccgt tgtcggtgat
     2161 ggccgggcag ttaagtccgg ggcaacacga gttcagtgtg gcagccggtt tgcctgacga
     2221 tgacagtgac ctgaaaggcg gcgtgtttgc ggcgtcatac ggttacggtc tggacgggct
     2281 gacgctgcgc accggggggg tgtttaacca ggactggcag ggcgtcagcg ccggggctgt
     2341 gctggggctg agttacctgg gggcggtgtc tgctgacggg gcttatgcca cggcgaaata
     2401 ccgtgatggc agccgcagcg gaaataaggt gcagttatcc tggagtaaac aacttgagat
     2461 gacgaacacc ggactgcggg tgagctggtc acggcagagt gaggaatatg aggatatgtc
     2521 ctcctttgac ccgacagagt tgtggtcgca gtcaaatcat ggtcgccgga cgaaggatga
     2581 atggaatgcc ggtgtcagtc agccggtggg tggtctgttc agtctgtcgg tgtccggctg
     2641 ggagcggagt tattaccccg catccatgac cgggagttac cggtacagcg atgacaacgg
     2701 taaggacacg gggattaccg gcaccctgag cacacagatt aagggtgtca gtcttaacct
     2761 gggctggtcc ggttcacgga acacccgggg ggaaaacaac tggtctgcat cggcgtcagt
     2821 atcagtaccg ttcacactgt ttgaccgtcg ctacagcagc agtacgtcgg tgagtacgag
     2881 taaagacggt ggcacaggct tcagcactgg tgtgtccggc tcgctgaatg accgtttcag
     2941 ttatggtctg ggcggtggtc gtgacagtga tggcggtgcc agcagttatc tgaacgcgtc
     3001 gtacagtggt gaccgggctt atctgagtgg tgccctgaac cactcgcagt ccggcggaac
     3061 cagtggttct gtctcggtca gcggttcggt actggccgtt ccggcggcga aagacattat
     3121 gttcagccgc acgaccgggg ataccgtggc cgtggtgaac gtgaaggata cccccggagt
     3181 gaaggtgacg tccggtgacg gacagactga cagcgacggc aacctggtgg taccgctgaa
     3241 cagctatgac tggaacacgg tgacgattga tgcgggcaca ctaccgctga gtaccgaact
     3301 gacgaacacc agtcagaagg tggtgccaac ggacaaggcg gtggtgtgga tgccgtttga
     3361 tgccctgaaa gttaagcgtt acctgctgca ggtgaagcag cgtgacggtg agtttgtgcc
     3421 ggggggaacc tgggcacgtg acagtaagaa cacaccactg ggctttgtgg ccaacaatgg
     3481 tgtgctgatg attaacacgg tggatgcgcc gggtgatatc accctgggcc agtgccggat
     3541 acctgcggcg aaactgcagg atactgagaa actacaggag attacgtgtg agtaagcgta
     3601 acgcagtaac gacgtttttc actaaccggg tgacaaaagc actgggaatg acgctggcgc
     3661 tgatgatgac ctgtcagagt gcgatggctt ccctggcggc agaccagacc cgctatatct
     3721 ttcgcgggga caaggatgcg ctgaccatca cggtcaccaa caatgacaag gagcgtacct
     3781 ttggtggtca ggcctgggtg gacaatatcg tggagaagga cacccgtccg acttttgtgg
     3841 tgacaccgtc cttcttcagg gtaaaaccga atggtcagca gacactgcgt atcatcatgg
     3901 cctcggacca tctgccgaag gataaagagt cggtgtactg gctgaacctg caggatattc
     3961 cgccggctct ggagggcagc ggtattgcgg tggcgctgcg cacgaagctg aaactgttct
     4021 accgaccgaa ggcactgctt gaaggccgca agggggcaga ggaagggctc agcctgcaga
     4081 gccgcccgga tggcaggacc atgctggtga acaccacgcc gtacatttat gcgattggca
     4141 gcctgctgga cggaaacggg aagaaaattg ccacggataa tgagacggca cagaaactgc
     4201 tgatgttcat gccgggtgat gaagtacagg tgaagggaaa tgtggtgaaa gtggattctc
     4261 tgagtgatta cggtgaactg cagacctgga caattaacca gaaaaaaacg tcagcgtctt
     4321 ccggggagaa ggtatctgat tcgtcggtta atttgcctga taaagcagat aaagcagata
     4381 aaaaataacc gccctccgga acacggaaca gaggggtaat aaatgaagaa aacaatgatg
     4441 gcagccaccc tggtcctgag tgcactcagt attcagtcag cacttgccgc tgagtacagt
     4501 gaaaagacac agtacctggg ggtggtgaac ggtcaggtgt caggtaacag tgtggtgaag
     4561 gtgacccgta caccgacaga cccggtgctg taccgctcag gcagtaacag cccgttgcct
     4621 gcagagctga taatccggca tgcagaaagc cgcccggctt ccggcggact ggcaaacatc
     4681 acggtgaaac agactctgcc ggataacggg gaagcccgca tcaccctgaa gacggccctg
     4741 atggttgacg gaaagagagt gacactcagt gccagacagc agggtgagga tgtggtgata
     4801 actgtacctg aggcgcagaa gctggttgag ttaagaacgg atgcaccggc agagctggaa
     4861 gtcccggtca gttaccgggg aaatctgcag atagcgctgc aggtggaaga ctgagaattc
     4921 atctccgacg acaaatgcaa tacatccgtg actccggaac gggtcatatt ggtaaaggga
     4981 tttaccgtat ttttaaacgg gaataacgta aagctattct gagttaa
//
LOCUS       DAJOTB010000242         4984 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJOTB010000242 DAJOTB010000000
VERSION     DAJOTB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.242"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            265..1485
                     /gene="arcA"
                     /locus_tag="OHN28_004659"
     CDS             265..1485
                     /gene="arcA"
                     /locus_tag="OHN28_004659"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCQ2299923.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDDNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            1496..2428
                     /gene="arcC"
                     /locus_tag="OHN28_004660"
     CDS             1496..2428
                     /gene="arcC"
                     /locus_tag="OHN28_004660"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2299924.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            2452..3456
                     /gene="argF"
                     /locus_tag="OHN28_004661"
     CDS             2452..3456
                     /gene="argF"
                     /locus_tag="OHN28_004661"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCQ2299925.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDETLVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            3513..4916
                     /locus_tag="OHN28_004662"
     CDS             3513..4916
                     /locus_tag="OHN28_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCQ2299926.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     MAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
BASE COUNT         1247 a         1201 c         1248 g         1288 t
ORIGIN      
        1 caattaacaa cgaagaataa ctctgcgtat atttgcgtat taatgcatta tcgtattttg
       61 tttctttatc atttatcaga accgcatcaa atattattaa cttcgattat taatatcacc
      121 gctggtgtga aagtcgtcac attttctgga agaaataaac tgaatcattg catctgaaaa
      181 ttactgaaca gacctgacta caagcattta atttgtttgc agccatctcc tctttatttt
      241 tgaaaaagtg ttaaaggaat aatgatggaa aagcattatg ttggttctga aattggacaa
      301 ctacgcagcg ttatgttgca tcgcccaaat ctcagtctga aacgtctaac accatccaat
      361 tgccaggagt tgttatttga cgatgtattg tccgttgagc gggcaggtga agagcatgat
      421 attttcgcca acacgctgcg ccagcaaggg atagaagttc tgttactcac tgatttacta
      481 acacaaacat tggatatccc ggaagccaaa agctggttac tggaaacaca aatatcagat
      541 taccgactcg gccctacgtt tgctactgac gttcgcacct ggcttgcaga gatgtcacac
      601 cgggaccttg ctcgtcattt aagtggtggg ctgacttata gtgaaattcc cgcctcaatt
      661 aaaaatatgg tggttgatac ccacgatatt aatgatttca ttatgaagcc gttaccgaat
      721 catttattta cgcgtgatac ctcatgctgg atttataacg gtgtatcgat taatccaatg
      781 gcaaaacctg ctcgccaacg tgaaaccaat aatctacgcg ctatttatcg ctggcatcca
      841 caatttgctg gcggcgaatt tattaaatat ttcggcgacg agaatattaa ttatgaccac
      901 gccacgttag aaggtggtga tgtgctggtg attggtcggg gggccgtgtt gatcggcatg
      961 tctgaacgca ccacgccaca aggagtcgag tttcttgctc aggcattgtt taaacatcgt
     1021 caggctgaac gggtaatcgc tgttgagctg cctaaacacc gctcctgtat gcatcttgac
     1081 accgttatga ctcatatcga tatcgacacc ttctcggttt atcccgaagt tgtgcgcccg
     1141 gatgataatt gctggacgct aacacctgac ggtcacggcg gcctgaaacg tactcaagaa
     1201 agcacactcc tccatgctat cgaaaaagcc ctcggtattg accaggtacg tttaatcacc
     1261 acggggggtg atgcctttga ggcagaacgc gaacagtgga atgacgctaa caatgtgctc
     1321 acgcttcggc cgggagtggt agtgggctac gagcgcaaca tctggactaa cgaaaaatat
     1381 gacaaagcag gcatcaccgt tttacccatc cccggtgatg aattaggccg cggacgtggc
     1441 ggcgcacgtt gcatgagttg ccctttgcat cgtgacggta tctaaggaag agatcatgga
     1501 aaacaaacca acactggtta tcgctctcgg cggtaatgcc ctgcttaaac gaggcgaacc
     1561 actcgaagca gaaatccaac gtaaaaatat cgatcttgcg gctaaaacta tcgcccaact
     1621 gactcaacac tggcgggttg tactggttca cgggaatggc ccacaagttg gacttctggc
     1681 acttcaaaat agcgcctatg cccatgtcgc tccctatcca ctcgacattc tgggggccga
     1741 aagccagggg atgatcggct acatgctcca acaggcgctg aaaaatcaac taccacaacg
     1801 tgaaatcagc gtgctgctga cgcaggttga agtggatgcc aacgatcctg ccttcagtaa
     1861 cccaactaag tacatcggcc caatttatga ccacgctcag actcaggtac tacaggcaga
     1921 aaaaggctgg gttttcaaag cagatggtca ctctttccgc cgggtcgtac cttctccaca
     1981 acctaaacgc atcgtcgagc gggatgccat ccagacactg atcgcccacg atcatctggt
     2041 gatctgtaat ggtggcggtg gtgttccggt ggttgaaaaa gccgatggat accatgggat
     2101 tgaagcggtt atcgacaaag atctttcagc cgcgctactg gcaagtcaga tccatgctga
     2161 cgccttgttg atcctcactg atgcggacgc agtatatctc gactggggca agccaaccca
     2221 acgcccgctg gcccaggtca cgccggaatt actcaacgaa atgcagtttg atgccgggtc
     2281 tatggggccg aaagtcaccg cctgcgcaaa gtttgttagt cagtgccggg gcattgcagg
     2341 gatcggctct cttgcagatg gtccggaaat tctcgccgga gacaaaggca ctcttattcg
     2401 ccttgatacc ccccataacc acgcttgatc catttttata aggacatccc catggctact
     2461 tcactgaaaa accgtaattt tcttaagctg cttgattaca ccccagcaga gattcaatac
     2521 ctgattgatt tggctatcaa tcttaaagcc gccaagaaat ctggaaacga aaaacaaacg
     2581 ctggtcggta aaaacattgc gttgattttt gaaaaatcct ctacccgtac ccgctgtgcc
     2641 tttgaagtcg cggcctttga tcaaggtgcg caggtgactt acatcggccc cagcggctca
     2701 caaatcggcc ataaagaatc aatgaaagat accgctcgcg tactcggacg catgtatgac
     2761 ggtattgagt atcgcggtta tggtcagaat atcgttgaag aattaggtga gttcgctggt
     2821 gttccggtat ggaacggact gaccaatgaa tttcacccga cacagatcct cgcggattta
     2881 atgaccatgc tggaacatgc gccaggcaaa acgctgccag agctgagctt tgcgtatctt
     2941 ggtgatgccc gaaataacat ggggaactcg ctgatggttg gtgctgcaaa aatggggatg
     3001 gatatccgtc tggtggcacc aaaatctttc tggccagatg aaacgctggt aacacaatgc
     3061 cgtgagatcg ccagcgtgac tggggcgaga atcaccctga cagaggatgt ggaagaaggc
     3121 gtatacgacg ttgattttct ctatacggat gtttgggttt cgatgggaga gccaaaagaa
     3181 gcctgggcag aacgagtgag tctgatggca ccttatcaga tcaatcaaca ggtgattact
     3241 gccacgcgta accctgaagt taaatttatg cactgcctgc ccgccttcca taatgagcac
     3301 accacagtag gacgcgagat cgagatggcc tacggcctca aaggactgga agttactgaa
     3361 gaggtttttg aatcggccca ctctattgtt ttcgatgaag cagaaaaccg gatgcacacc
     3421 attaaagcgg tgatggtagc aactctcggc gactaaccct gacgggcatg cgtcgacgag
     3481 tagcgtatgc ccgtgattat ggagatcaca ttatgggtaa attcaaattt cccaccgctt
     3541 ataccatctt gttcgtgctg attgcgttag ttgcggcaat gacctggatt gtacctgcgg
     3601 gaaaatatca gatggtgaca aatgccgcac tgggaaagga agttcctgtt gcaggcactt
     3661 acgcacctgt tgaggcacaa ccacagggta ttacggctgt cctgttagcg cccatcgacg
     3721 gcttgtacaa ccatcaaacc tacactgcgg gagcgattga tgtcgcgctg tttgtcctga
     3781 tcatcggtgg atttttggga gtagtaaata aaaccggggc aatagatgcc ggtatcgaac
     3841 gagtgacaac tcgccttaat ggtcgggaag agtggatgat tccgatcctg atggcgctct
     3901 tcgctgctgg cggtactatt tatgggatgg cggaagaatc actccccttc tacacgttgc
     3961 tggtgcccgt catgatggct gcccgctttg atcctctggt cgctgccgcc acagtgttat
     4021 tgggatcggg cattggcacg ctgggctcaa ccattaaccc atttgctacc gtcattgctg
     4081 ccaacgccgc agggatcccc tttactcagg gcattttgct gcgtgttatt ttgctgttag
     4141 taggctacgt gatttgtgtt tactgggtta tgcgttatgc ccgcaaagtt cgcaacagtc
     4201 cggaaagctc tatcgtggcg gataagatgg ctgaaaacca ggcgcatttt ctgggaaatc
     4261 gttcagaaac gatgctggaa tttaccccca cgcgcaaagc gatcctgata ctttttgctg
     4321 catcctttgc ctttatgatt tacggcgtag cggtactggg ttggtggatg gcggaaatct
     4381 ctgctgtctt tctggcagct gcggtcatcg tgggtgtcat tgcccgcatg ggcgaagaaa
     4441 ccttcaccag tacctttatt gacggcgcgc gggatctcct tggtgtggca ctgattatcg
     4501 gcatcgcccg cggtattgtg gtcgtgatgg ataacggcat gattacgcac acaattctgc
     4561 acagtgctga gaatctggtt tcagggcttt ccaccactgt tttcatcaac gtgacctatt
     4621 ggctggaagt attgctctct tttctggttc cctcatcttc cggccttgca gtgttaacga
     4681 tgccaataat ggctccactc gctgattttg ctcatgtaca acgcgatctg gtggtgaccg
     4741 cttatcagtc tgcttccggg gtggttaatt tgattacgcc aacctctgcg gtagtgatgg
     4801 gcggtctggc gattgcccgt gtaccctggg tccgttatct gagatgggta gcaccgctac
     4861 tgctgattct cacgatgttg aatatgattg tgctgagtat cgccgcgatg atttaacgcg
     4921 acaggccgct tatcaaagcg gccttcaaat ctggtgtagt gataaatgaa ccactctcac
     4981 atta
//
LOCUS       DAJOTB010000243         4942 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJOTB010000243 DAJOTB010000000
VERSION     DAJOTB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.243"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1019
                     /gene="ychF"
                     /locus_tag="OHN28_004663"
     CDS             <1..1019
                     /gene="ychF"
                     /locus_tag="OHN28_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCQ2299927.1"
                     /translation="GIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTME
                     FVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSGKVNPADDIEV
                     INTELALADLDTCERAIHRVQKKAKGGDKDAKAELAVLEKCLPQLENAGMLRALDLSA
                     EEKAAVRYLSFLTLKPTMYIANVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAVEAD
                     IAELDDEERDEFMQELGLEEPGLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGATA
                     PQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNF
                     LFNV"
     gene            1234..2409
                     /locus_tag="OHN28_004664"
     CDS             1234..2409
                     /locus_tag="OHN28_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCQ2299928.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            2520..2804
                     /locus_tag="OHN28_004665"
     CDS             2520..2804
                     /locus_tag="OHN28_004665"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2299929.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            2801..3655
                     /locus_tag="OHN28_004666"
     CDS             2801..3655
                     /locus_tag="OHN28_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2299930.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(4278..4634)
                     /locus_tag="OHN28_004667"
                     /pseudo
     CDS             complement(4278..4634)
                     /locus_tag="OHN28_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(4731..4928)
                     /locus_tag="OHN28_004668"
     CDS             complement(4731..4928)
                     /locus_tag="OHN28_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299931.1"
                     /translation="MSNPGPLAALVYGFILIVLLSVSLILYSIGRTIKNEIGKGIAYA
                     LTGILLILSLLVGAFILTLLT"
BASE COUNT         1338 a         1204 c         1248 g         1152 t
ORIGIN      
        1 ccggtattga agcggccaac tttccattct gcaccattga gccgaacact ggcgtcgtac
       61 caatgcctga ccctcgcctg gatcaactgg ctgaaatcgt aaaaccgcag cgtacacttc
      121 ccacgaccat ggaatttgtc gatatcgccg gtctggtaaa aggcgcatcg aaaggcgaag
      181 gtctgggtaa ccagttcctg accaacatcc gtgaaaccga agcgatcggt cacgttgttc
      241 gctgctttga aaatgacaac atcattcacg tttccggcaa agttaacccg gcggatgata
      301 ttgaagttat caacaccgaa ctggcgctgg cggatctcga cacctgtgaa cgtgcgattc
      361 atcgcgtaca gaagaaagcc aaaggtggcg ataaagacgc gaaagccgag ctggcggtac
      421 tggaaaaatg cctgccccag ctggaaaacg caggtatgct gcgcgcgctg gatttaagcg
      481 ctgaagagaa agcggctgtt cgttacctga gcttcctgac gctaaaacca acaatgtaca
      541 tcgccaacgt caacgaagac ggttttgaaa acaacccgta tcttgaccag gtgcgtgaaa
      601 tcgcagcgaa agaaggttct gttgtagttc cggtttgtgc tgctgttgaa gcagacattg
      661 ccgaactgga cgacgaagag cgtgacgagt ttatgcagga gcttgggctg gaagagccgg
      721 gcctgaaccg tgtgatccgt gccggttata agctgctgaa cctgcaaact tacttcaccg
      781 ctggggtgaa agaagtgcgt gcatggacca ttccggttgg tgcaaccgcg ccgcaggcag
      841 cgggcaaaat ccatactgat tttgaaaaag gctttatccg tgcacaaacc atctcgtttg
      901 aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga agcaggcaaa atgcgtgcag
      961 aaggcaaaga ttacatcgtt aaagatggcg atgtgatgaa cttcctgttc aacgtctaaa
     1021 ttaaatctgc tgtctcatga ggtttcactg aatctcataa gaaccgtaaa actctataaa
     1081 atccacgcaa ttgcgtggat ttttcatttc gcaatgtctc aattcatctc ataacaacgc
     1141 tgtcaaaaat gagtacatgg atgagtacaa cattcttcca tcttcatttt aagtgagtac
     1201 aataacggta taataaataa acaaggcccc gttatgctca ctgatacgca atgccgcact
     1261 gctaagccga aagaaaaact ctatcgactt aatgatttca atggtctcta cctcgaagtg
     1321 aaacccaatg gtaaaaaggc atggcgctat cggtttaaac tcaacggtaa atccagcatg
     1381 tttgcgctgg gtgagtaccc gacggtaaaa cttgccgaag ctcgcgagaa atgcgagcaa
     1441 gcgcgtaagc aagtcgcaga tggagttagc ccaacacagg ctcgtcagtt agataagatc
     1501 cgcaaggtca acgacgcatc caacaccttt gagctaatcg ctaaagaatg gttgcagatg
     1561 aaagactggg ccggaattac caaaacgcgg cgactggata tgcttgaacg tgtggttttt
     1621 cccgccatcg gtaagctccc tgtcagagag atcactccac accatatact taaaattctt
     1681 caggaaaccg ctaaacgagg agcaccaaca gttgccgctg aagcccgtcg caccatttca
     1741 tcagtttttg agttggcagt cgcgacgctt agagctgata gcgatcccgt atggcctgtg
     1801 cgcaaggccc ttccggctaa taagacacag cacaaacagg cactaaatcc ccaacaaatc
     1861 ggaaagttac taagctgttt tgacaatagt cgtggctcgt atcaggttaa ctattgcatg
     1921 tggcttatgt ggtggacatt ggcgcgtcct gcagaagcca ccgaagcaga atggacagaa
     1981 ttcaatcttg ataacgccct atggactatt ccggcagcgc gaatgaaagc ccgcagagaa
     2041 cacgttattc ccctaccctt ccaagctgtc aaaatgctca gaacactaca gggattaacc
     2101 gggcatcggc agcacctttt cccgggcaga gacaatccgc taggtccaat gacatcgcac
     2161 tcactgcgcc agctacttaa aagcctcggc tggagtggta catatagccc acatgcaacc
     2221 cggaccacag gaagtacccg tttaaacgag ttgggctatc gacctgatgc tattgaggcc
     2281 caacttgcgc acgctgatac caacaatgtt cgtcgcacat acaaccatgc aacttatctt
     2341 gatgagcgta aagtgatgat gcaggaatgg gctgatatgt tggacaaatg ggtagcagga
     2401 ttcaagtaga acttgctgca caaagtgaac cacgaggata gaacttattc ctcaaggttc
     2461 tggagtgccc ccaagcccgt agacaaatct gccctatagt ttgagcatag gaggagtcta
     2521 tgggcacacc acgatttacc ccagagttta aggaagaagc tgtccgtcag gtcactgaac
     2581 ggggctattc cgttgccgat gtttctgaac gcctcggcgt atcggctcac agcctttata
     2641 agtgggtccg gtcagtcaga ccggataaca gcgggcatca ggcgcaggat ttactggatg
     2701 cgcggacgga gattctcagg cttaaagcgc agcttaaacg tactgaggaa gaacgggata
     2761 ttctgaaaaa ggcagcgcgg tactttgcaa gggagcccga ctgaagtacc gcttcatcaa
     2821 cgatcaccgt gaaatctggt cgatcgtaac aatgtgtcgg gttctgaagg tcgcccgtgc
     2881 gggattttac gtctggcttc atcgccctgt ttctgccgga gaaaaggata atcagcggtt
     2941 gctggagctc atccgcgatg cttatgcact gagcggcgga gtctacggtt atcgcagggt
     3001 tcacggcgat cttcgtgaaa tcggtgaagt atgcagcaga aaccgtgttg ccagaatcat
     3061 gaagcggaac aggatacagg ccgtacatgg ctataaggtc ccgcgtggcg tcaggggaag
     3121 gccgtcactg atagctccta atcgcgtaca gagggaattt acgatggtaa aacccaacca
     3181 ggtgtgggtg actgatatta cctacatccg cacctggcag ggttggctgt atctggcggt
     3241 ggttatcgat ctttttgccc gtaatgtggt gggctggtcg atgaaaccaa cgctgtcgcg
     3301 cgagctggca ctggatgcgc tgctgatggc agtctggcgg cgtaagcctg cagaaaacgt
     3361 catagtgcac agcgatcagg gcagtcagta tggcagtgat gactggcagc ggttctgcag
     3421 ggccaacaat ctggcgccga gtatgagccg tcgtggcaac tgctgggata atgcggtggc
     3481 agaatcgttc ttcagttcac tgaaaaaaga gcgcatcaga aagcggatat ataaaacccg
     3541 ggacatggcc cgggcggata tcttcgatta cattgaagtg ttctacaacc ggacccggcg
     3601 ccacagtcat ctgggtggcg tcagcccgga ggccttcgaa aaggcctcac cgtgaggaca
     3661 gaaactgtct accggaatgt ggtcactcca cttgagtcgt cttgctgtac tcctttggcc
     3721 aatccattta gctctccaac gtgatgtttt caacggtaac aaaagtaaca atataacatg
     3781 cagtcatttc aacgatctca gtgttaccac ccattttgag caatcatatc aaagtaacaa
     3841 aaggggaata ttgtgtggtt catcgctgaa agcaggctaa gatcacctgc cggctatgca
     3901 tatcatgaaa gtgctaaatg tcccgtcttg tttatcaacc gagataagct gtaattgact
     3961 gagtggctcc ccttaatgct ttttctcaat caacagatat ggatgggacc agtcagtgac
     4021 ctctttatat gaaaaaaacc catcgtgctc cagtgcgcaa acgcccttat gactggcaac
     4081 gtaatcgtaa acagcccggc tttccagtcc ctgtaacatg tgcgaaagca gctttcggcg
     4141 catcacatct gacttcctgc gtttactgtc aacaacgggt aataaagtga tccactaacc
     4201 gtcaccacca acggtttaat attgatccac cttgtttact caggattagc ttcaggtata
     4261 accccggcct ttcgtttcta cttcagtcga tagctctctc cttttatctg gactacgtgt
     4321 gagtgatgta agatacggtc cagcatcgcc gatgtcaggg ctgcatccct ggcgaacgtc
     4381 tgatcccact gcccgaacgg caggttggag tccaggatca tcgcgctcct ctcgtaacgt
     4441 ttagcgatga cctggaagaa cagcttggct tcttccagac tgaacggcag ataacctatt
     4501 tcatcgatga taagtagctt cggggccatg acaccgcgat tgagtgtcgt tttgtaacgg
     4561 ccctggcgct atgaagtgga tagctgtagc agcaggtccg ctgttgtgaa gcgaaccttg
     4621 atgcctgccc gtagcggcgg acgcccatgg cacaggtgcc gcaggcgagc atgcgttcaa
     4681 cggagagtcg ggtccattag ctgatgctgt cagcgtattt ttcgaggaat ttatgtaaga
     4741 agcgtcaaaa tgaaagcgcc aactaataat gacagaatta ataaaatacc agtaagcgcg
     4801 tatgctatac cttttccaat ctcgtttttt atggtacgtc caattgaata aaggattaaa
     4861 gataccgata gcaaaactat caaaataaaa ccatacacta acgctgcaag tgggcctggg
     4921 ttactcataa tcatccttta tg
//
LOCUS       DAJOTB010000244         4886 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJOTB010000244 DAJOTB010000000
VERSION     DAJOTB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.244"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            17..514
                     /gene="ftnA"
                     /locus_tag="OHN28_004669"
     CDS             17..514
                     /gene="ftnA"
                     /locus_tag="OHN28_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCQ2299932.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(552..791)
                     /locus_tag="OHN28_004670"
     CDS             complement(552..791)
                     /locus_tag="OHN28_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCQ2299933.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            982..2193
                     /gene="tyrP"
                     /locus_tag="OHN28_004671"
     CDS             982..2193
                     /gene="tyrP"
                     /locus_tag="OHN28_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCQ2299934.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2244..2909)
                     /gene="yecA"
                     /locus_tag="OHN28_004672"
     CDS             complement(2244..2909)
                     /gene="yecA"
                     /locus_tag="OHN28_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCQ2299935.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3381..3800)
                     /locus_tag="OHN28_004673"
     CDS             complement(3381..3800)
                     /locus_tag="OHN28_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299936.1"
                     /translation="MVVKNGLACGIMLALVGCATKPVSNEQATPVPEKQIISTALLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
     gene            complement(3967..>4886)
                     /locus_tag="OHN28_004674"
     CDS             complement(3967..>4886)
                     /locus_tag="OHN28_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="late control protein"
                     /protein_id="HCQ2299937.1"
                     /translation="GSSKQTDELRVAIVSETLKIPARGVKISIGLGFGNQIIDKGIYI
                     VDGASSGGEPRVVDFTAKAAPMNAAKGSPTVQSKKTRSWSDVTVSDIVATIASDNGLK
                     PRVSKRFADNVITQLDQVGESDMHLMSRLATRFDAVSKPAGGYWIFLPRGAGESVSGA
                     PLQHYTLTRRDNSSWGYSRNGQSGDSGSGGENPEPTYLIKYHDTATGQIKELRTGSGG
                     DPVIEWPAVEPSLDAAKEAAPGLKGGAAKKEFSMTYTTPATLDLVSLTAECKVTTQGF
                     GTEEDRDWTINTLSLTLAENGFSVRLSLE"
BASE COUNT         1150 a         1258 c         1133 g         1345 t
ORIGIN      
        1 tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa
       61 cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac
      121 cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca
      181 gcgtctgttt gattacctga ctgataccgg caacttaccg cgtattaata ccgttgaatc
      241 tccgtttgct gaatattcct cacttgatga attattccag gaaacctata aacacgaaca
      301 attaatcacg cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc
      361 aacatttaat ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa
      421 atcgattatt gataaattaa gcctggcagg caaaagtggc gaaggtctgt attttatcga
      481 caaagaactc tctaccctcg acacacaaaa ctaatgctct ccggcggcag gttttctgcc
      541 gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg ttgaaaaacc ttcttctgaa
      601 ggtataaatt tgccttttgc tgccagaaac gccaccaact ctcccgcagt catcccttct
      661 gccgagcagg tgtgaaaacg tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga
      721 ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga
      781 atagagtcca tcgttcctcc tcaaaaaaag cctagcgtag cgattgctgc ttatgaagac
      841 tttgcgccag cgcagaactg aacgcttttt attgtacatt tatatttaca ctatatgtaa
      901 cgtcgatttg acgaatcagc cgttatgcct taacctgcgc cgcagatatc actcataaag
      961 atcgtcagga cagaagaaag cgtgaaaaac agaactctgg gaagtgtttt tatcgtggcg
     1021 ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggcagg tgttggtttt
     1081 agcgtcacgt taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta
     1141 ctgctggagg tgtatcagca tgtcccggca gataccggtc tgggcacgct ggcaaaacgc
     1201 tatctgggac gctacggtca gtggctgacg ggcttcagta tgatgttctt aatgtatgct
     1261 ctgactgcgg catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg
     1321 acaggtattt caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc
     1381 gtggtttgtg tcggaacttc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa
     1441 attatttttc tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt
     1501 ttaaccctgc cgttgcaaca gggactggca ctgtctgcaa tcccggtgat ttttacgtcg
     1561 tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa cattcgtaag
     1621 ctacgctggg tgtttataat cggtagtgcg atccccctgg tggcatatat tttctggcag
     1681 ctggcgaccc ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgcc
     1741 ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg
     1801 gcagtgcatt tatttgctga tttagccctc gccacgtcct tcctcggcgt agcgttaggc
     1861 ttatttgatt atctggcaga tttgtttcag cgttcaaata ccgttggtgg acgattgcaa
     1921 actggtgcaa ttacctttct gccgccactg gcgtttgcac tgttttatcc acgaggattt
     1981 gtgatggcgc tgggttacgc cggtgtggca ctggcggtac tggcattgat tatcccttcg
     2041 ctgttgacct ggcaaagcag gaagcacaat cctcaggcgg gttaccgggt gaaaggtggt
     2101 cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg
     2161 attgcggcag gcttgttacc agaagtgggg tgattagatt cttattgggt gccagaatta
     2221 acgctgacac ccaatttgac ctcttaatgc aggcagcact gcttaaattt cttaccacta
     2281 ccgcacgggc aaggatcatt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca
     2341 gctttttcct gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg
     2401 gcatctacgc tctcttcaaa cgcttctggc gacatcttct ctacccgctc gaagttttcc
     2461 tcagtaccgt gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac
     2521 cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc
     2581 tcgctgccat cgacttctcg caagccgaat aatggctcaa actgctccgg aaattcgttc
     2641 agacgctctg cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct
     2701 ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt
     2761 gccggttcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc
     2821 acatcaagaa tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag ccattccaac
     2881 tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag
     2941 cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt
     3001 aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt
     3061 tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg
     3121 acttgaaccc gcacagcgcg aacgccgagg gattttaaat tggtcgcttt gtgcaataaa
     3181 ttcaaataat tatggcaaat tatccggaca taatggtgtg tatgaatatc aaataatcat
     3241 atagttacga cgcgtcttac tcatcgtctt ctgaaggttt ttggcaaaaa tggtgatgtt
     3301 ctgcgccttt atgtgaccaa actgtcagga gcaaagcgag gtaaaggggt atgagaaaaa
     3361 ctcacatacc ccaacacaaa ttaaaacgcg gttgggtgaa ttccatattc accgtttgat
     3421 ccatacccta tgcgatacat caaagtacga tctgttgtca ccttgcccgc ttgttcgctc
     3481 atgccaccac cacatactcc ttttggccac gcgctgaaga catgatcacc aatcgttggg
     3541 tatacaacga ctttctgagc tgtacctaaa tctgccacct ctttaccatc aacataaaca
     3601 cgggaaaaac acgcgcttcc aaagaaaccc gagtcacgct tgatgatcac ctctcccgta
     3661 ccgtctcgtt tttcgagtaa cgcggtactg ataatttgtt tttcaggaac aggagtggct
     3721 tgttcatttg agacaggttt tgtagcacaa ccgacaagag caagcataat cccacaggca
     3781 agaccatttt taacgaccat gtttgaaaac tcccaatatt aaaaatatgt cagcatctaa
     3841 cgataacaac gttcagcacc agagactctt cgttctccgg cttacgccta aaaatatcgc
     3901 aggtgccgtt tgcagtcgac atctgttaat actaaattca taccgccacc ggctcgccga
     3961 gcccatttac tccagagaca gccgaaccga aaaaccgttt tcagcgagcg tcagactcag
     4021 tgtgttgata gtccagtccc ggtcctcctc ggtcccgaaa ccttgagttg tgactttgca
     4081 ttctgctgtc agcgacacta aatcgagcgt cgctggcgtc gtgtacgtca tagagaactc
     4141 ttttttagct gcaccgccct tgagccctgg tgcagcttct ttcgctgcgt cgagcgacgg
     4201 ctcaactgcc ggccattcaa tgacaggatc gccgccacta cctgtccgca attctttgat
     4261 ctgcccagtt gctgtgtcgt gatatttaat cagatacgtc ggttccggat tttcgccacc
     4321 gctaccacta tcgccacttt gtccatttct cgaatagccc cagctggagt tatcacgacg
     4381 agttagcgtg tagtgctgga gcggagcacc gctaacagat tcgcccgcgc cgcgcggcag
     4441 gaatatccag tacccgcccg cgggtttact caccgcatcg aatctcgttg cgagccgcga
     4501 catcaggtgc atatctgact cgccgacctg atcgagttgc gtgataacat tgtctgcaaa
     4561 ccgctttgat acgcgtggtt ttagcccatt atcgctcgct attgtcgcaa cgatatcgct
     4621 gacagtgacg tcgctccagg aacgagtctt tttgctctga actgtcggag atccttttgc
     4681 tgcgttcatc ggagccgctt ttgctgtgaa atcaactacc cggggttcac cgccactgct
     4741 agcaccgtca acgatgtata tccctttatc aattatctga ttaccgaaac cgagcccaat
     4801 actgattttt actccgcggg ccggaatttt cagagtttca gaaactattg ctacgcggag
     4861 ttcgtctgtc tgtttactgg agccgc
//
LOCUS       DAJOTB010000245         4795 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJOTB010000245 DAJOTB010000000
VERSION     DAJOTB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.245"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..554
                     /locus_tag="OHN28_004675"
     CDS             <1..554
                     /locus_tag="OHN28_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCQ2299938.1"
                     /translation="PILSNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTTPSRG
                     CTLAAALAFVAYLLNLRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNFQSAR
                     VTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEIHLRI
                     PLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(562..2043)
                     /gene="clsC"
                     /locus_tag="OHN28_004676"
     CDS             complement(562..2043)
                     /gene="clsC"
                     /locus_tag="OHN28_004676"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCQ2299939.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(1985..2518)
                     /gene="ymdB"
                     /locus_tag="OHN28_004677"
     CDS             complement(1985..2518)
                     /gene="ymdB"
                     /locus_tag="OHN28_004677"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCQ2299940.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2613..2924)
                     /locus_tag="OHN28_004678"
     CDS             complement(2613..2924)
                     /locus_tag="OHN28_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2299941.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3045..3377)
                     /gene="csgC"
                     /locus_tag="OHN28_004679"
     CDS             complement(3045..3377)
                     /gene="csgC"
                     /locus_tag="OHN28_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCQ2299942.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3437..3895)
                     /gene="csgA"
                     /locus_tag="OHN28_004680"
     CDS             complement(3437..3895)
                     /gene="csgA"
                     /locus_tag="OHN28_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCQ2299943.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3936..4391)
                     /gene="csgB"
                     /locus_tag="OHN28_004681"
     CDS             complement(3936..4391)
                     /gene="csgB"
                     /locus_tag="OHN28_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCQ2299944.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1230 a         1191 c         1045 g         1329 t
ORIGIN      
        1 cacccattct gagtaacggc atgttcaatt ttattgtcat gcaaacgtta ttttatttgc
       61 cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc ttgtttacca
      121 cgccttctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat ttactcaacc
      181 tgcgctatgg cagtggcgat gcctggatgt atgaaaccga gtcggtgatc accatggtcc
      241 tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt aacttccagt
      301 cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt caccacccgt
      361 taacgctgtt tttcggcgca tacattacac cacacatcac ctccaactgg cttggttttc
      421 tctgtggcct gatattcgta gtagggattg cgataattct gtatgaaatt catttacgca
      481 tcccattatt gaagtttttg ttctctggta aaccggttgt taagcgtgag aacgataaag
      541 cacctgcccg ttaagccgcg tttacaataa ccattccacg ggcaatatcg acgccagttt
      601 gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa tctctttctt
      661 tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct gccaggccgc
      721 atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt cgctctctat
      781 cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga aagagccaat
      841 aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggcac cggaattacc
      901 ggttataccg cgatcgtgta atgtactact ttgttcacgc gtcggcttga gttcatataa
      961 ttccacccca tagcgaagca attttttgcg ccagcgcgcg tatccggcat ggacgacagc
     1021 aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt ttctcaccat
     1081 ccgtaagagt tgcgccacac ctgcacgtgt cggtacaaaa taggaagaga taatatcgat
     1141 gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt
     1201 tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgttttcg cccagataag
     1261 cggcaacgtt ccatcaacca gatgatgtat aaacggactg gattccattt tgcgtaagta
     1321 acgatgcgtc atgacatcgt tatgccagga ggcgggtaac tcgatgtgat ccgccatttc
     1381 accttccggg acatccagca cctgctgtaa gggggaaacc gatttgcaat accagtagcg
     1441 ggcgaaatca tcggcaacgt cctctaccac gggtcctatt gccatgacat ctaaatccga
     1501 aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgtc ctcccaccag
     1561 cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga gacgggaaaa
     1621 gtcggtgata taaccaagcg gacgtagcaa acgaaacgag aaaggattaa aaagccggac
     1681 ttcaatgcgt ggatggctgt caagcaagcg taaaatgtca tcaagtccgg gcgtattgtt
     1741 gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa
     1801 cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc
     1861 tgccatttca gccagacgat accgggcggc aaacgcatcc agacttttct ccagaggaaa
     1921 aagtccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg ccagccgagg
     1981 caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg ttttcttcat
     2041 cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg
     2101 tttttactgc gatttcagcc gctgccgcac ggggataacc ataaacccca gtactgattg
     2161 caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgtaggcta ttgagatagg
     2221 catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc ccgacggtgt
     2281 gcactacggc tttagcggga agatcgcctg caagcgtaat aacggcatgc cccgtagggc
     2341 aatcgccctg ctgctgcctg acttttaaac aggcatccag cagggccgga cccgctgcgc
     2401 gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc gcattcacaa
     2461 tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc gttttcataa
     2521 cgcctcctta caccttcttg tttcctaagt gtacagcagg ctttttagtt aaatcgcatt
     2581 tttctgctta gcaacgtgag ttgttcagaa acttagttat attcgatgtt cattacagcc
     2641 agctttttct gtttatcaag atactgcact tttactgagg ctatttgcac gtttttaaca
     2701 ttgccgctca ttaaggcttt gctggaatag gttttacgcg gaacagaacc attctgtggg
     2761 cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg
     2821 ccataaaaat ggatcacgcc cccttgcgcg gtgcttcctg caatggcaat aaaaggaaaa
     2881 aacaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc tatttcctca
     2941 atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg attcgcagca
     3001 gaccattctc tccaggttca tcttatgctc gatatttcaa caaattaaga cttttctgaa
     3061 gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt aacaactatt
     3121 ttcacccgat cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat gggttgatta
     3181 gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact gccttcgcgc
     3241 agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc aggaataatg
     3301 gtgtacatat ccccttgctg ggtcgtatta aaggttatct gactggaaag tgccgcaagg
     3361 agtaataacg cattcatatt cttctcccga aaaaaaaaca gggcttacgc cctgtttctt
     3421 tcatacagat gatgtattag tactgatgag cggtcgcgtt gttaccaaag ccaacctgag
     3481 tgacgttaac gctggagtta gatgcagtct ggtcaacagc agcaccgtta ccgccaccga
     3541 actgtttaac agtcatagta gaatctttac cattccactg atcaagagta gcgctgttgc
     3601 cgaaaccacg ctgagtcaga tcgattgagc tgtcatcaga accttggcca acatctgcac
     3661 cattaccgcc gccgtgctgg gtaatggtca gatcagagtt gcgggcgtca gcttgcagag
     3721 caagagcaga gttaccgcca ccgtactggt aaatattcag ctctgaattc gggccgctgt
     3781 tattaccgcc accaccgtgg ttgccgccac cgccgccgta ctgaggaaca acacctgcca
     3841 gagcgctacc ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa
     3901 acccccatcg gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc
     3961 atttgcgact gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt
     4021 gctttattac cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa
     4081 atactggcat cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc
     4141 tggtcaatct ttgcccggtt gctactacct tcttgcgcaa caaccgccaa aagttttgag
     4201 cctccctgcc gtaactgagc actattatta gtcccagctt gaccaattat ggctgcctga
     4261 ttaaatgaag acttactcaa ttcatttacc gcaaagttat attctgaatt agctaaatca
     4321 taacctgctg cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat
     4381 ttgtttttca tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa
     4441 cttggttgtt aacgcaaccc gtattttgtt aacgctgcgt tacgatggaa agtatgtctg
     4501 cggaaatatt tttaataact cacccgcgcg ttgtattttt ctttttttgt tcataccaaa
     4561 gtattaaaac ccacgtaaat acgctgttat ctacgcaaaa aatattttgt tttcttttaa
     4621 atctccgttt tccgctatca aaaatcacca gacagtcatt cttcttgccc gtcgctgatt
     4681 gctgcgatat gtcttgcgca caagccgtga caaacctcgt ctttttgcag taaaaaattg
     4741 tccacggtgg tatggagaaa aaacaagaac gttttacatg acgaaaggac tacag
//
LOCUS       DAJOTB010000246         4782 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJOTB010000246 DAJOTB010000000
VERSION     DAJOTB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.246"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            64..705
                     /locus_tag="OHN28_004682"
     CDS             64..705
                     /locus_tag="OHN28_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299945.1"
                     /translation="MFDLYQHIADNFLQKKAEDNLHVIYTGASIKYIESIFGPPVKEE
                     HSEDGRVHEYIYSFKKFYLQVVYDNNNTVILYSVTSKDENFHPEVPYLRKTLGHKFKD
                     FGSDIDYLQSSYSSKFYQYEEGHYLGNPGNYRNFYLAYNPAGVDYFELDPLPDLSNDD
                     KSPPNKNDLEKFRNNNAPNTFGVGDIHGGPEGLELSFGLGIDYYDARDIPDKD"
     gene            complement(823..1632)
                     /locus_tag="OHN28_004683"
     CDS             complement(823..1632)
                     /locus_tag="OHN28_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299946.1"
                     /translation="MSLPVPFGAIVTVVKKCWSTLTQIMLIKTYQLSHERWAVRHRLG
                     ASWHSLGNYLEYSLRFAQLTDKEPRCSRIAFRSKEERLQQVELVFEAIGSGLRYQETL
                     CIHDVNNSPIILTLSNIPTLDVLILDDGFAFTVEQYQLKYCTIKLMTGESISIDNSPK
                     YSLMQNWCLNDTWKRRWGIIWNCNAIECARRRIAEYWKWGFCLPLVGRPPLYSPSRKG
                     IHLFEAKPLRWFMTRPWCLNIQFWTAIWSELFILSDENALQWRWKEPPQQD"
     gene            complement(2201..3373)
                     /locus_tag="OHN28_004684"
     CDS             complement(2201..3373)
                     /locus_tag="OHN28_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299947.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAIKRHKQQLGAIEEYTQWL
                     KKEPRASYFFLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNSGLTPIPEKRSGM
                     PPEYLRFATGFPPQPKIAGPKGKPMRTVHPDKIYREIIWFCSGYLRKSGPEATRTIIN
                     SIFCEWASIFNDYSSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRR
                     DERADGSVPLKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(4473..4628)
                     /locus_tag="OHN28_004685"
                     /pseudo
     CDS             complement(4473..4628)
                     /locus_tag="OHN28_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(4672..4782)
                     /locus_tag="OHN28_004686"
                     /pseudo
     CDS             complement(4672..4782)
                     /locus_tag="OHN28_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1218 a         1079 c         1075 g         1410 t
ORIGIN      
        1 ttaattactg tttttttgag tggtattctt gctgttcttg gatattttgt tgcattgaac
       61 ggagtgtttg atttatatca acatatcgca gataattttt tgcaaaaaaa agcagaggat
      121 aatcttcatg tgatttacac cggagcgtca ataaagtaca tcgagtcaat ttttggccct
      181 cccgtgaagg aagaacattc tgaggatggg agggttcatg agtatattta ctctttcaaa
      241 aagttctatc tgcaagtggt ttatgataat aataacacag tgatactcta ttccgtaacc
      301 agcaaggatg agaattttca tcctgaagta ccatatctga gaaaaaccct tggacataag
      361 ttcaaagatt ttggaagtga tattgattat ttacagtcga gctattcttc gaagttctat
      421 caatatgaag aagggcatta tttaggaaat cctggcaact atagaaattt ttaccttgcc
      481 tataaccctg ctggagttga ttattttgag ttagatccac ttccagatct ctctaatgac
      541 gacaaatcgc ctccaaataa aaatgatctt gaaaaattca gaaataataa tgctccaaat
      601 acatttgggg ttggcgatat ccacggaggt cctgaggggc ttgagttaag ctttggactt
      661 gggattgatt attacgatgc gagagatatt ccagataaag attagtgatc atgtatcata
      721 ccatttttat atcgtttcaa attaggtagt cacaattaga ctagttaatc taaccagcac
      781 tgaggtccgc tcctcgctca gagcggacgg ccttcgtgaa agttagtctt gttgtggtgg
      841 ctctttccag cgccattgca aggcattctc atcgctgaga ataaacagtt ctgaccaaat
      901 tgccgtccaa aactggatat tcagacacca tgggcgtgtc ataaaccagc gtaacggttt
      961 tgcttcaaac agatgaatac cttttcgcga tggtgaatat aacggggggc gtcccactaa
     1021 cggcagacaa aagccccatt tccaatattc agcaatacgc cttcgggcac attcaattgc
     1081 attacagttc cagatgatac cccaacgccg tttccatgta tcattgagac accagttttg
     1141 cattagcgaa tatttgggcg aattgtcaat agatatactt tcaccagtca ttaattttat
     1201 cgtgcaatat tttaactggt actgttcaac cgtaaatgcg aatccatcat ctagaataag
     1261 tacatcaagt gtaggaatat ttgaaagcgt taatattatc ggtgagttat ttacatcatg
     1321 aatgcatagt gtttcttggt agcgtaagcc actgccaatg gcttcaaaaa caagttcaac
     1381 ttgttgcagt cgttcctctt tgcttctgaa agcaatacgt gaacaacgag gttctttatc
     1441 tgtcaactga gcaaatcgta gactgtactc caaataattc cctaaggaat gccagctcgc
     1501 ccctagcctg tgtctcactg cccagcgctc atgtgagagt tgataagttt ttatcagcat
     1561 tatctgggta agagtagacc agcatttctt cactactgta acaattgcac caaaaggaac
     1621 aggaagtgac attactaaac ccctacaaat atcagataat taggttttat atcatttcag
     1681 tcagtgtaat caaatgataa tcaggattgg ggctaatctt gaacgccact aaagatcggc
     1741 aacaatgggg atccaatgtt tcgttagtga tttttcgcat tagcagatac atgcttactt
     1801 ccgctcctcg ctcacaacgg accttcagtt cagccgctcc ggccgcttca tgccatgaat
     1861 gtacgcagcc agtcagaata ttaaggtgcg gatttcagtt cacttgtcag cgccagtgac
     1921 acaagacagt aatgaccaac aatttggtat tgacacaaca agttgcggcc ccggttcgtg
     1981 gaggagatta aacgactgta gccggggcca ctgaaatgaa gaattcagag agactgaatg
     2041 atatgtcgca ccttcagtga atagtgtaaa aaccgggata ttctcatccg ggattaccca
     2101 ctcatactca atgcgtaact ttagtgcggc ccccacaggt aagagccgga cacattcgtt
     2161 gtgtatgctg cagaatcggt aaccggaggc cctgcggtcc ttaactcagt gtcttgtgtt
     2221 gctcatttgc cacaaagtcc tggtacgcct gctcaatcat ttcattcagc agtttttttg
     2281 ggaggacacc acgacttcgg gcaatacttt caagctgttt gtggattttt ccggacagtt
     2341 tcagcgggac agaaccatca gcacgttcat cacgcctttt ttgttgctcc cgggcacttt
     2401 tcagctcatc aagaagaatc gctttatgct gaatcctagg ggcctggaag gaggcctgcg
     2461 taaacttcgc tctgttccgt ctgggattac tttccagtaa aaactgtacc tgttgctcat
     2521 tccagccttc ccagttatca aacgtggcgc aggcgaacca gtatttctgc tccggggtaa
     2581 ggggattcag cggggttccc acacaccgga cctgcatggc gttccataac cagtcacact
     2641 gttcactgtc cctgctgtcc acccacgaaa agggggagga gtaatcattg aagatgctgg
     2701 cccattcaca gaatattgag ttgatgatcg tcctcgtagc ctctggtccg gatttccgca
     2761 ggtaaccgga gcagaaccat ataatttcac ggtagatttt atccggatgc acggtgcgca
     2821 tcggctttcc tttgggaccg gcaatcttcg gttgcggtgg aaaccctgtg gcaaaccgca
     2881 gatattccgg gggcattcct gaacgttttt caggaatggg tgtgagtcct gagtttctgg
     2941 tcttttccgc ttgtccggta ttgcctccgg ccggagtgtt gtcgggagta tcttccggag
     3001 gcgaggtgtt ttcattagtt gggggaacaa cagcagaact gtccatctcc ggaatgggtt
     3061 gaggggcata atcactgtgg tagcccgggg aaagcgttaa gtcccgaagg gtgacatcag
     3121 gttccgggca gtgagtgcca ccgggagtga acgggagttg ttgcttttgg gggaaaaatg
     3181 tattgtgaat gcgggtatac agccggaaca gaaaaaaata agatgcccgt ggttcttttt
     3241 ttaaccactg tgtatattcc tctatggcac cgagctgttg tttgtgccgt tttatagcat
     3301 cgatatacgg acagctggtg cagatatcat ccagttcctt tcccacaata cacctcacct
     3361 gctcttccca catttgcaga ttttaagaaa gcaaacaaaa acctccgtac atgatgtaca
     3421 gaatgtacag aacaatctgg caggtactgg cgtcagaaga gctccgggcc gcctgcactt
     3481 tccggaatgc gccggttatg aaaaaccgga aattcagtct ctgttgtaag atgtaccctc
     3541 tgatactacg ttttcgtttt ctctgtgtat ttaactcact gtatttattg tctttttaag
     3601 cctgactggt attctccggc catagtgtgc cggatatata tcccgtaatc ttctcatttt
     3661 tatataaaaa tacctgcact atcttcagcg catctgtctc ctttaagtaa aaaatgtttt
     3721 cacgtattgc tgtaaattca ttttaaatca gtgtattacc ttgatattgc tgtctcctgg
     3781 tttgatgcgt aatcgttctg cctttctgga tgattctggt gcttccgggt gcattctcct
     3841 ctgcacatgc aacagttcgt catcgccacg ttcccaaaaa gcatataaaa acgaatcgaa
     3901 tttgatattt ttttcaatcg tttttcatag gaatttcgta gggtgttctc cgctataacc
     3961 tccatatttg ctgtcttttt cttcataaat cgtatgcaat gtagcctgtc tgtcaagacc
     4021 tcttctctgc tgttgaggag acgatctcat ttttggtctt ttccgggaga gaggctgtca
     4081 gatgagcgag ccggatttac tgtcagaggt acaccccgta gcagacctgt gctcccattt
     4141 ccgcgatccg gaaccaacaa cgccctacgg ttaatactat ttgaagcgtc aggtactgca
     4201 ggaagttccc ccctgcgcgg acatcggttc ctgccgacat ctcacaatac ggtcagactg
     4261 tcatgcagta acacccgcaa tgcggtctgc ggttttaagt gcaccgataa tgcgtcatag
     4321 taagtctggg ggagttacgg aatgcctgtt aagtcgtgcc gtgggtggag acagcttact
     4381 tttccacagc aactgcaatc attcaggatg atcaggagat attctgtcaa tgtcacgatc
     4441 ctgccatttt cgtacccgct aagatacttc cgttactgat gctgtccgct tttcaccatt
     4501 tcatcctcac tgacaggcgt tgcgttttgg gtcatcacca cagctttacg tttctctttt
     4561 aaccggtaac gctctccttt gatattcagc gtggttgagt gatgcagcag tcaatccagg
     4621 agctctgttg tccggcaacc accatatcaa cttcttcccg ggtgtggcgg agaggtggta
     4681 tcctgactga gtttcgaagc gaaccgttct ttttgacggg cgcattttac gtccgggctg
     4741 aatgtagtaa cgcggcatgg aacgtcggcc cgtatggccc at
//
LOCUS       DAJOTB010000247         4772 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJOTB010000247 DAJOTB010000000
VERSION     DAJOTB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.247"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            139..927
                     /locus_tag="OHN28_004687"
     CDS             139..927
                     /locus_tag="OHN28_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCQ2299948.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1133..1480
                     /locus_tag="OHN28_004688"
     CDS             1133..1480
                     /locus_tag="OHN28_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCQ2299949.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1474..2313
                     /gene="sul1"
                     /locus_tag="OHN28_004689"
     CDS             1474..2313
                     /gene="sul1"
                     /locus_tag="OHN28_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCQ2299950.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2441..2644
                     /locus_tag="OHN28_004690"
     CDS             2441..2644
                     /locus_tag="OHN28_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299951.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2800..4005
                     /gene="chrA"
                     /locus_tag="OHN28_004691"
     CDS             2800..4005
                     /gene="chrA"
                     /locus_tag="OHN28_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HCQ2299952.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     TGWLLSVAL"
     gene            4016..4321
                     /locus_tag="OHN28_004692"
     CDS             4016..4321
                     /locus_tag="OHN28_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2299953.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            4365..4430
                     /locus_tag="OHN28_004693"
                     /pseudo
     CDS             4365..4430
                     /locus_tag="OHN28_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4548..>4772
                     /locus_tag="OHN28_004694"
     CDS             4548..>4772
                     /locus_tag="OHN28_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCQ2299954.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWR"
BASE COUNT          917 a         1311 c         1405 g         1139 t
ORIGIN      
        1 aaggctaaca atgcgttgca gcaccagtcg cttcgctcct tggacagctt ttaagtcgcg
       61 tctttgtggt tttgctgcgc aaaagtattc cacaaagccg caacttaaaa gctgccgctg
      121 aacttaacgt taggcatcat gggtgaattt ttccctgcac aagttttcaa gcagctgtcc
      181 cacgctcgcg cggtgatcga gcgccatctg gctgcgacac tggacacaat ccacctgttc
      241 ggatctgcga tcgatggagg gctgaagccg gacagcgaca tagacttgct cgtgaccgtc
      301 agcgccgcac ctaacgattc gctccggcag gcgctaatgc tcgatttgct gaaagtctca
      361 tcaccgccag gcgatggcgg aacatggcga ccgctggagc taactgttgt cgctcgaagc
      421 gaagtagtgc cttggcgcta tccggcgcgg cgtgagcttc agttcggtga gtggctccgc
      481 cacgacatcc tttccggaac gttcgagcct gccgttctgg atcacgatct tgcgattttg
      541 ctgaccaagg cgaggcaaca cagccttgcg cttctaggcc catccgcagc cacgtttttc
      601 gagccggtgc cgaaggagca tttctccaag gcgcttttcg acactattgc ccagtggaat
      661 gcagagtcgg attggaaggg tgacgagcgg aacgtcgttc ttgctcttgc tcgcatttgg
      721 tacagcgctt caactggtct cattgctcct aaggacgttg ctgccgcatg ggtatcggag
      781 cgtttgcctg ccgagcatcg gcccctcatc tgcaaggcac gcgcggcgta cctgggtagc
      841 gaggacgacg acctagcaat gcgcgtcgaa gagacggccg cgttcgttcg atatgccaaa
      901 gcaacgattg agagaatctt gcgttgagcg gcatgtgcga aaagtgcatc gacccgcgcc
      961 gagggcatct gatgcctaac tcggcgttca agcggacggg ctgcgcccgc cgctcaacta
     1021 tgcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     1081 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     1141 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
     1201 tagcgagggc tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc
     1261 attttatttt ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg
     1321 gtcgggactc ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct
     1381 tgatgcgtgg ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc
     1441 cccatcgtgg aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat
     1501 ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc
     1561 gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc
     1621 catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta
     1681 gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag
     1741 cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct
     1801 gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca ctcagcgcag
     1861 cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct cgacgagatt
     1921 gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg
     1981 ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac atcgctgcac
     2041 gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg
     2101 tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct gggtccagcg
     2161 agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg cacccacgcg
     2221 cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt tcgcagtcgc
     2281 gacgccagag accgagggtt agatcatgcc tagcattcac cttccggccg cccgctagcg
     2341 gaccctggtc aggttccgcg aaggtgggcg cagacatgct gggctcgtca ggatcaaact
     2401 gcactatgag gcggcggttc ataccgcgcc aggggagcga atggacagcg aggagcctcc
     2461 gaacgttcgg gtcgcctgct cgggtgatat cgacgaggtt gtgcggctga tgcacgacgc
     2521 tgcggcgtgg atgtccgcca agggaacgcc cgcctgggac gtcgcgcgga tcgaccggac
     2581 attcgcggag accttcgtcc tgagatccga gctcctaggg atcgcctcag aaaacggaaa
     2641 ataaagcacg ctaagccgta agtaagcgtg ctcctgtgaa agccacagct aaaactgcgt
     2701 agtacacata gaggtatttt tttctagatt tatatcgaat ttcgttatcg aatttcgctt
     2761 attattgatc aactcgttgc agatcatgaa ggcgaaagta tgacgaataa ccctaccgat
     2821 gacagcagac catggtcggt ctttcttatt tttctgcggc ttggattgac atcttttggc
     2881 ggccccattg cgcacttggg ctacttccgc gccgaatttg tcacacggcg gcgctggctc
     2941 tccgaacgga gctatgctga cttggtcgcg ctttgtcagt tcttgccagg gcctgcaagc
     3001 agccaggtcg gcatagcggt aggactgtct cgggctggat acagcggggc gctggctgct
     3061 tgggctggct tcacgctgcc gtctgccata gccttgatcc tttttgcgct cggcatctcc
     3121 agctatggcg attacgtctc gcagggcgcg ttgcatggct taaaagtggt ggctgtggcc
     3181 gtggtcgctc aagcagtatg gggcatggcg cgtaacctat gcacggatgg gctgcgagtc
     3241 accatcatgg caattgctac ctgcgtcgtt ttacttgtgc cgtccgcgtg gggacaggtt
     3301 ggcgtgattg ctatcgcagg catcgcaggc cggttattgt tcaagccagc gaaagttgtt
     3361 gagcatgacc ccctacctat cacggtcagt caccgggccg gcgtgctttg gctctcgctg
     3421 ttctttgtct tgctgattgg cctgccggtg ttggccgaac tgatgccaag tcaaaccatg
     3481 gcaatggtgg attccttcta tcgtgtcgga tcactggtgt tcggcggtgg tcacgttgtg
     3541 ctgccattac tgcaagccga agtggtgccc tccggctggg tcaacaatga atcctttctc
     3601 gcggggtacg gggcagctca agcggtgccc ggccctttgt tcacgttcgc cgcgtttctt
     3661 ggtgcctcga tgaacaccgc cccgtcgggc tggatcggcg gcattgtgtg tctgctggct
     3721 atcttcgcgc cctcgttctt gctggtcgtc ggatcaatgc cattttggga gcgtttgcgc
     3781 cgcaatacag gcatccaagc tgcgctggcc gggatcaatg ccgctgtagt cggcttgctg
     3841 ctggccgcgc tgtatcagcc tgtatggact agcgccatct ttcagccgca agacttcggc
     3901 ttggcattag ttgcccttgt cgcacttatg ttctggaagc tcccgccgtg gctggttgtc
     3961 ctcggtagcg gtgcaactgg gtggctgttg agcgtcgctc tgtgaggcac aaaaaatgac
     4021 tgacaaagac ctctacggcg gtttgatccg cctgcacatc cttcaccatg cagccgagga
     4081 acctgtcttt gggctgggga tcatcgaaga gctacgccga cacggctacg agatgagcgc
     4141 tggcaccgtg tacccgatgc tgcacggcct ggaaaaaaaa ggctatctga cctcacgcca
     4201 cgaacgcacc gggcgacgtg aacggcgtgt ttatgacatc actgaacaag gcagaactgc
     4261 acttgctgat gcgaaaacaa aggtcaagga gctgttcgga gagttggtag aaggtggttg
     4321 agtttttgct tctatgtcag gttgagctat accctaactg gattgtcatt ttcagaagac
     4381 gactgcacca gttgattggg cgtaatggct gttgtgcagc cagctcctga cagttcaata
     4441 tcagaagtga tctgcaccaa tctcgactat gctcaatact cgtgtgggct ctgttgcaaa
     4501 aatcgtgaag cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca
     4561 agtggcgcca tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc
     4621 cgatcagcta tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata
     4681 cgacgatcta tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt
     4741 tctggcggcg tggctttgat ccgagctggc gc
//
LOCUS       DAJOTB010000248         4715 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJOTB010000248 DAJOTB010000000
VERSION     DAJOTB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.248"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..671
                     /locus_tag="OHN28_004695"
     CDS             <1..671
                     /locus_tag="OHN28_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCQ2299955.1"
                     /translation="KKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHKIQPNAQ
                     GTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDGDDDND
                     SVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILESLLK
                     KSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYESLPW
                     DIFK"
     gene            668..1321
                     /locus_tag="OHN28_004696"
     CDS             668..1321
                     /locus_tag="OHN28_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="HCQ2299956.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNM
                     KILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSG
                     VLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLN
                     DENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
     gene            complement(2310..2504)
                     /locus_tag="OHN28_004697"
     CDS             complement(2310..2504)
                     /locus_tag="OHN28_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCQ2299957.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFETESETGT"
     gene            3580..4536
                     /locus_tag="OHN28_004698"
     CDS             3580..4536
                     /locus_tag="OHN28_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299958.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERI
                     YQIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSP
                     ENDIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDI
                     DEAKQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDY
                     CGGEEHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQ
                     EAGKYRGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
     gene            4520..>4715
                     /locus_tag="OHN28_004699"
     CDS             4520..>4715
                     /locus_tag="OHN28_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCQ2299959.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKG
                     GTLLGVKHGSDRYTKDIDFST"
BASE COUNT         1361 a          871 c         1065 g         1418 t
ORIGIN      
        1 aaaaaaaact ttctgaaatt gacttggggg attttttcaa taggtttaat gactcaatgg
       61 atgttgctga ggctgaattg ggatatggta ttaaatgtaa agttcaatct cataaaattc
      121 aacctaatgc acaaggaaca tttccaactg tacagataaa tatagccttt tgcgatcgtt
      181 ctaatgcgtc tgcaatgaaa aggcttgaat ctaatcaatc accttcagtg ataaatatcg
      241 attttagctt taacgaaaaa acgtatgatt ttgacttttt atcattagat ggtgatgatg
      301 acaatgatag cgtaaagacc tacagcttaa ctgatttgat ggctgaaaaa tatcgatccg
      361 ttttgcaaca aaaagttaga gagagaaatc gagagcaaga tatatatgat atcaattatc
      421 ttttaggtaa atatgagttt tctcggcaag ataagtataa aatactggag tctcttttaa
      481 aaaagagtga aggtaaacaa ttggataatc tcttaaacgt taaaggtata agggatgtag
      541 aaattatcga gcgttccagt cagcgatatc aggatttatc atccaccgtt aaatcattac
      601 ctctttttga agatgcttat gaaaaagttg cctgtttcta tgaaagtctt ccgtgggata
      661 tattcaaatg atagcgttca gttttagtga tattggtaac agatgtgaaa acttagactt
      721 attatctacg ataaattgtc aaaatgatgt gtttggaatg gttattatgg atgggtattc
      781 ctgctctgag tggaatatga aaattctctc tggtaggtta gataagtgcg tgttttctgg
      841 tgatctgaat aaagatatta ttgaaccatg caagtcagtt cctgttaagg ctgcagtatg
      901 tttccttttt aaaaataata cgcacctcca tatatataac gcaggagatt gccgtgtata
      961 taacaaaagt ggagtccttc taacagagga taactcggtt gcctggaaaa gattaaagga
     1021 aaatggaatc gataataaaa aaatacctca cctcgtttgt tattgcccag acagaaatta
     1081 tctaacatca tttgttgatt tctgctctga tgagataatt ttcaatcatt cagtttttga
     1141 tttaaatgat gaaaacaaag aggttatttt atgtactgat gggttttggg agcattttaa
     1201 tgatggtact gttattgacc tcatgaaaga taacccagaa tctaacttgg ctaaatttac
     1261 taaaaatatc ccgggtaacg cagagaatca tacggtttgc tacgttagat tcccttcttg
     1321 atttcgtgtt ttttgaagaa gctattgaga tcttcgtgtc atatagataa tctcttttct
     1381 gtaaaagaaa aaaaccgcct ttgcggggcg gttttctctt aggttcaaag agtttgcggc
     1441 tcgaagaacc aaggtaactg gcttggagga gcgcagtcac caaaacctgt tctttcagtt
     1501 tagccttaac tggcgcatga cttcaagact aactcctcta aatcagttac cagtggctgc
     1561 tgccagtggc gttaaggcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1621 ggcgcagcag tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1681 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1801 gccaccaggg ggaaacgtct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttctgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgcctgcggc
     1921 gcggccttct tcctgttctt ggtcttttgc tcacatgttc tttccggcct tatcccctga
     1981 ttctgtggat aaccgtgctg ccgtttttgt gtaagtcagt accgctcgcc gcagccgaac
     2041 gaccgagcgt agcgagtcag tgagcgagga agcggaaaag cgcctggacg cgcattttct
     2101 ccttacgcat ctgtgcggaa tttcacaccc ggcatggggc acttttcata caatctgcat
     2161 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
     2221 tcgccccgac acccgctgac gcgccctggc gggcttgtct gctgccgaaa acgctcgcgg
     2281 acaagccgtg agcgcctccg tcagcttcgt tatgttcctg tttcactttc ggtctcaaag
     2341 cgaacctgga tgctgttctg gagttcttcc gcgagttcgt gcagtcgttc gcacatggcc
     2401 gcctgttcgt cggcatccag agcatccagt ttttcaagca gtgtcaggct ctggcttttg
     2461 ataaatcgcg ccatattcag cgcagtttgt tgctgtttgt tcaccttccg ttgttctccg
     2521 tcagtaccgg gtgtcgggtg gaccctgacc aggtggtaat tgtaagacgt tgcgggcgac
     2581 ggtattacaa ttgcacatcc tgtcctgttt ttttaggacg tatcatggca cagcatatca
     2641 actgataaat aatccctgtg cagtgcagcc gctgttgcat aaaatttcgc tctgcgtcga
     2701 gtgtttctta atcaataatt ccgctgatac tccggaaaaa cgcgagtctg ttctcatgcc
     2761 tgaaatgccc acaccccacg caaaaacaat tttttgctga tttttcttta taaatagaga
     2821 gttatgacaa attagttctt cttgctctct ttgtgatatt taaaaaagcg gtgtcggcgc
     2881 ggtgttgtag ctgcgccaac accgcttttt aggggtggta ctgactattt tcataaaaaa
     2941 catcatttta tattaggggt gctgctagcg gcgcggtgtg tttttttaca ggacgcccct
     3001 gggggcgctg ctaggggtgt ctgttcagat atgtagcaat gagtcaggga ttcacgaact
     3061 gaaaatgtcc gaaaatgtgt ctcatgggca ccgtatgcgc ccaaaccgat cgccattcac
     3121 tttcatgcat agctatgcag tgagctgaaa gcgatcctga cgcatttttc cggttttcgc
     3181 ttggtaaaac atctcttttt gcggtgttcg cggcagaatc gcgttcagcg cgttttagcg
     3241 gtgcgcgtaa tgcgacgtta tggtaaatga cgctgaaaaa tgatctttta cgctcatcgt
     3301 ttatgagcaa cagatgtcct aatcatggat ttgaactata accgccgctg aattgatcgc
     3361 atgaggtgtt atgaaaacgg tgatataagt aaactttttc ttgacttcct acttattatc
     3421 ctataaaata aatcctaagg atttatttta taggatatca aaaatataaa aatggcaaac
     3481 gcttgtttgt tattattttt ttcttgacaa tcaatctcta atagaatata cgtcctaagg
     3541 acgtatattc tattagagac taaaccgaaa aggttgaata tgtatcttat cgaagaagcg
     3601 ctaacgtctt ggttgttgaa tgaatataaa aaaccagtca taaccaaaga agaaatctct
     3661 ttgcagattt acctttttta tttaaaaaaa gaatataatg gtgagaggat atatcaaatt
     3721 agaaagaaca aagcaggacc taatgaattt gaaaaaaata tattaaaatt attaaagtca
     3781 ggggttttat caagagttga aagtaacgat aagcatcatg cttaccccca tttaattggc
     3841 ggtttctatg aggtcttttt tgttaaaggt aacgatggtt ctccggaaaa tgatatagtg
     3901 tgctctacgt atccatatgg atacttatcc tatttatccg ctatggattg gcataacatc
     3961 acagataaaa tacccaagat aatccacttt acgacatgtc gttccgaaca gtggaagaat
     4021 aagttctatc atgattttca aaaggataat cctgatatag acgaagccaa acaattcatg
     4081 cctagatttc caagaaattc tgaggtcaat ggaataaaaa ttatcgttac acaagaaaca
     4141 aattatgttg agccaatgaa ggctcgtaat tcccctgtca gggtatcatc tatcggtaag
     4201 acctttatgg atatgaccag gcggcctgat tactgtggtg gtgaggagca tgtattggaa
     4261 tcatttattg agcacggtaa aaaatatgca cctctgatta taaaggaagt cgataagctt
     4321 ggccgtcata ttgacaaggt aagggttgga ttcattttgg atagaattat tggggttaat
     4381 aataaaaaaa taagcgaatg gaaacaagag gcgggtaaat acagaggtag ttcgaaagtg
     4441 ctatctccaa agagtccatt ctcaccttac tttgatgaag aatggtttat ttctattaat
     4501 gttgatatgg ttaagtctta tggaaaaaga aattaataat tggatcgaag agtctggtgg
     4561 gaagtacagg ttgatgcggc aggctgtaca tataatactt aaatctattt ctatagagga
     4621 gagtttgaat gaaatgatgg ttatgaaggg agggactctt cttggtgtta agcatggtag
     4681 tgatagatac acaaaggata ttgatttttc taccg
//
LOCUS       DAJOTB010000249         4710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJOTB010000249 DAJOTB010000000
VERSION     DAJOTB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.249"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            161..1843
                     /locus_tag="OHN28_004700"
     CDS             161..1843
                     /locus_tag="OHN28_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCQ2299960.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1895..3052
                     /locus_tag="OHN28_004701"
     CDS             1895..3052
                     /locus_tag="OHN28_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2299961.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            3064..3975
                     /locus_tag="OHN28_004702"
                     /pseudo
     CDS             3064..3975
                     /locus_tag="OHN28_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            4337..>4710
                     /locus_tag="OHN28_004703"
     CDS             4337..>4710
                     /locus_tag="OHN28_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2299962.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT         1449 a         1013 c          956 g         1292 t
ORIGIN      
        1 acttagtaga acagaaagta atgtcaacgc gttatggact gttatttccc tttttttagc
       61 taaatctgct atctctttat gtgactaact tcacttacat tcacttattt ctcttcgtaa
      121 aattactttg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
      181 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
      241 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgcgt tctcagactt
      301 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactga ccatggatga
      361 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      421 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcgcaaaa
      481 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      541 ggcgcacggt gtttccggcc cctcccgcgc cgcaataatg accggtcgag cgcccgcccg
      601 ctttggtgtc tattccaata ctgatgctca ggatggtatt ccgctaacag aaactttctt
      661 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttatc
      721 aaaaatcagt aatgtgccgg tgccggaaga taaacaaacg cgtgactatc atgacaactt
      781 cactaccttt tctgcggaag aatggcaacc gcaaaaccgt ggctttgatt actttatggg
      841 attccatgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa accgtgaacg
      901 cgtccccgca aaaggttata tcagcgatca gttaaccgat gaagcaattg gcgttgttga
      961 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
     1021 gccaaatgat aatcctgctc cggagcaata tcagaagcaa tttaataccg gtagtcaaac
     1081 agcagataat tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
     1141 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
     1201 tgcagttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
     1261 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1321 taattatgac aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga
     1381 tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaaa
     1441 taagaaacaa ggcgagccac ataaaaatct gacctggata acatcttatt ctcactggtt
     1501 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgtccgcc atcagtcaga
     1561 cgattacccg cataacccca acaccgagga cttaagccaa ttctcttata cggtgagaaa
     1621 taacgattat tcgcttgtct atacagtaga aaataatcag ttaggtctat acaaactgac
     1681 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1741 aggcgtggta agagagttta tcgataacag ccagccaccg cttagcgagg taaatcagga
     1801 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc tttatgcggc
     1861 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1921 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1981 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc
     2041 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     2101 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     2161 tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     2221 tgagtttctg gttggtatct cgatcgatgg cccccaagaa ctacatgacc gttacagacg
     2281 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2341 atatcaaata gagtttaata cgttaaccgt tattaataac attaatgtcc attaccctct
     2401 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2461 cgaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2521 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2581 ggttaaaaac gatgtgggtg aaattttcat ccgtcagttt gaaagctttg tcagccgttt
     2641 tttggggaat gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2701 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2761 aaacattaat aaatcggaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2821 acggatttca gcgaaatgtc agcaatgtgt atataaacct atctgcaatg gtggttgccc
     2881 taagcatcgt attactaaag taaacaatga gactgtttcc tatttttgcg aaggttataa
     2941 aatccttttt tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag
     3001 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     3061 ccccacagca cgattaacga caatatttgg gcatcgctac aaaatgctca aattcaggcg
     3121 ttgaaaacgc tcgaccagcg tccagcggag aaattcgccc agcagatgta tgagacgcgc
     3181 tatgctgata accgcacgaa attaccgcaa gcaaatcaga ttgcacagtt tactgccgca
     3241 gatgcactgg ctgccgatcg ccagttgttt tcatctccag cggatatcac gtttgtcatt
     3301 gtcggtaatg tcgcagaaga caaactcgtg gcgttaatta cgcgttactt aggatcaatc
     3361 aaacactctg attcgccatt agccgcaggt aaaccattaa ctcgcgcgac ggacaacgca
     3421 tcggttactg taaaagaaca aaatgaacca gtggcacagg tttcacagtg gaagcgttat
     3481 aattcccgga ctcctgttaa tctggcgacg cgtatggcgc tcgatgcttt taacgtcgca
     3541 ctggcaaaag atctacgcat taatattcgt gaacaggcat ccggggcata cagcgtttct
     3601 tctcgcctct cggttgatcc tcaggccaaa gatatcagtc atttgctggc ttttacttgt
     3661 caaccagaac gacatgatga actgttaacg ttagcgaatg aagtgatggt taagcgtctg
     3721 gccaaaggga tcagtgagca agaactgaac gaataccagc aaaacgttca gcgcagcctc
     3781 gatatccaac agcgtagcgt tcaacaatta gcgaacacca ttgtaaatag tcttattcaa
     3841 tatgacgatc ctgcagcatg gactgagcag gagcaattgt tgaaacaaat gacggtagag
     3901 aatgttaaca ctgctgttaa acaatatctt tctcatccgg tgaataccta taccggagta
     3961 ttattgccaa aataatcaca gccccgtcaa cacatcgttg gcggggattt catcaatatt
     4021 cgctattttt atgtaataat tttataaatg tgttcaaagt aaataaccgt gtaccaataa
     4081 tgatattata aggtctgatt tttacgtgat aattcaggag acgatagaaa acacataaaa
     4141 taacagcata aaacacctta cctccacaca gaattctcta ttatgttgtt ttttaataaa
     4201 aatacattat tcacgcaata tctcgcgata aataacatta ggattttgtt atttaaaacc
     4261 gagtcctttg cacttgctta ctttatcgat aaatcctact tttttaatgc gatccaatca
     4321 ttttaaggag tttaaaatgg ataagaagca agtaacggat ttaaggtcgg aactactcga
     4381 ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa tcaaaacgtt ttccgctgca
     4441 cgaaatgcgt gatgatgtcg cattccagat tatcaatgac gaattatatc ttgatggcaa
     4501 cgctcgtcag aacctggcca ctttctgcca gacctgggac gacgaaaatg tccataagtt
     4561 gatggattta tccattaaca aaaactggat cgacaaagaa gaatatccgc aatctgcagc
     4621 aatcgatctg cgttgcgtaa acatggttgc cgatctgtgg catgcgcctg caccgaaaaa
     4681 tggtcaggcc gttggcacca acaccattgg
//
LOCUS       DAJOTB010000250         4657 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJOTB010000250 DAJOTB010000000
VERSION     DAJOTB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.250"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1109
                     /gene="proV"
                     /locus_tag="OHN28_004704"
     CDS             <1..1109
                     /gene="proV"
                     /locus_tag="OHN28_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCQ2299963.1"
                     /translation="QILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMVRLLNR
                     LIEPTRGQVLIDGVDIAKISDVELREVRRKKIAMVFQSFALMPHMTVLDNTAFGMELA
                     GINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDILLMDEAF
                     SALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVVQVGTPD
                     EILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKLLQDEDR
                     EYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQTPLSELLSHVGQA
                     PCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1102..2166
                     /gene="proW"
                     /locus_tag="OHN28_004705"
     CDS             1102..2166
                     /gene="proW"
                     /locus_tag="OHN28_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCQ2299964.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2223..3215
                     /gene="proX"
                     /locus_tag="OHN28_004706"
     CDS             2223..3215
                     /gene="proX"
                     /locus_tag="OHN28_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCQ2299965.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3407..4591
                     /locus_tag="OHN28_004707"
     CDS             3407..4591
                     /locus_tag="OHN28_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2299966.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
BASE COUNT         1026 a         1236 c         1250 g         1145 t
ORIGIN      
        1 aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt ctggccattg
       61 aagaaggcga gatatttgtc atcatgggat tatccggttc gggtaaatcc acaatggtac
      121 gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat ggtgtggata
      181 ttgccaaaat atccgacgtc gaactccgtg aggtgcgcag aaaaaagatt gcgatggtct
      241 tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg ttcggtatgg
      301 aactggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca ctgcgtcagg
      361 tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg atgcgtcaac
      421 gtgtgggatt agcccgcgcg ttagcgatta atccggatat attattaatg gacgaagcct
      481 tctcggcact cgatccatta attcgcaccg agatgcagga tgagctggta aaattacagg
      541 cgaaacatca gcgcaccatt gtctttattt cccacgatct cgatgaagct atgcgtattg
      601 gtgaccgaat tgccattatg caaaatggtg aagtggtgca ggttggtaca ccggatgaaa
      661 ttcttaataa tccggcgaat gattatgttc gtaccttctt ccgtggcgtc gatattagcc
      721 aggtgttcag tgcgaaagat attgctcgtc gtacaccgaa cggcttaatt cgtaaaaccc
      781 ccggcttcgg cccgcgttcg gcactgaaat tattgcagga tgaagatcgc gaatatggct
      841 acgtcatcga acgcggtaat aagtttgtcg gcgctgtgtc tatcgattcg cttaaagccg
      901 cgttaacgca gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta gcggtcgatg
      961 cacaaacgcc tcttagcgag ttgctctctc atgtcggaca ggcaccctgt gcggtgcccg
     1021 tggtcgacga ggaccaacag tatgtcggca tcatttcgaa aggaatgctg ctgcgcgctt
     1081 tagatcgtga gggggtaaat aatggctgat caaaataatc cgtgggatac cacgccagcg
     1141 gcggacagtg ctgcacaatc cgcagacgcc tggggtacac cggctgctgc accgactgac
     1201 ggcggtggtg ctgactggct gaccagtacg cctgcgccaa acgtcgagca ttttaatatt
     1261 ctcgatccgt tccataaaac tctgatcccg ctcgacagtt gggtcactga agggatcgac
     1321 tgggtcgtta cccattttcg tcccgtcttt cagggcgtgc gcgttccggt tgattacatc
     1381 ctcaacggtt tccagcaatt gctgctgggt atgcccgcgc cggtggcgat tatcgttttc
     1441 gctctcatcg cctggcagat ttccggggtc ggaatgggcg tggcgacgct ggtttcgctg
     1501 attgccatcg gcgcaatcgg tgcctggtca caggcaatgg ttaccctggc gctggtgtta
     1561 accgccctgc tgttctgtat cgtcataggt ttgccgttgg ggatctggct ggcgagaagt
     1621 ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca tgcagaccac gcccgcgttt
     1681 gtttatctgg tgccaatcgt catgctgttt ggtatcggca acgtgccggg cgtggtggtg
     1741 acgatcatct ttgcactgcc gccgattatc cgtctgacga ttctgggaat taaccaggtt
     1801 ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca gcccgagcca gatgctgttc
     1861 aaagttcagt taccgctggc gatgccaacc attatggcgg gcgttaacca gacgctgatg
     1921 ctggcccttt ctatggtggt catcgcttcg atgattgccg tcggcgggct gggtcagatg
     1981 gtacttcgcg gtatcggtcg tctggatatg gggcttgcca ccgttggcgg cgtcgggatt
     2041 gtgatcctcg ccattatcct cgatcgcctg acgcaggccg ttgggcgcga ctcacgtagt
     2101 cgcggcaacc gtcgctggta caccactggc cctgtcggtc tgctgacccg cccattcatt
     2161 aagtaactct gcacttgccc ggtaacaccg ggcatatcac cctgccaaaa aaaggaataa
     2221 caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt
     2281 ttgctgccga cctgccgggc aaaggcatta cagttaatcc agttcaaagc accatcaccg
     2341 aagaaacctt ccagacgctg ctggtcagcc gcgcgctgga gaaattaggt tataccgtca
     2401 ataaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa
     2461 ccttcaccgc cgtgaactgg acgccactgc atgacaacat gtacgaagct gccggtggcg
     2521 ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc ggcacaaggt tacctgatcg
     2581 ataagaaaac cgccgaccag tataaaatta ccaacatcgc gcaacttaaa gatccgaaga
     2641 tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttaaccggc tgtaacccag
     2701 gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatggactg accaataccg
     2761 tgacgcataa tcaggggaac tacgcagcga tgatggctga caccatcagt cgttacaaag
     2821 agggtaagcc ggtgttttac tacacctgga cgccgtactg ggtgagtaac gaactgaaac
     2881 cggggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg
     2941 ccaataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagtacc atgcatatcg
     3001 ttgccaacaa agcctgggcc gagaaaaacc cggcagcagc gaaactgttt gccattatgc
     3061 agttaccagt ggcagatatt aacgcccaga acgccatcat gcatgacggc aaagcctcag
     3121 aagacgatat tcagggccac gttgatggtt ggatcaaagc ccaccagcag cagttcgatg
     3181 gctgggtgaa tgaagcgctg gcagcgcaga agtaattttt attcgggcgg ataaggcgtt
     3241 tttgcctcat ccgccgctct gtacacaatg cctgatgcga cgctggcgcg tcttatcata
     3301 cctacaaacc acaccgcaca tttcaacaat ctatctttca tcccatattc atcaacatcc
     3361 gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga ctaaacctaa
     3421 tcatgagctt agcccggcgc tgatcgtgct gatgtctatc gccaccggtc tggcggtagc
     3481 cagtaactat tacgcccagc cattgctcga caccatcgcg cgtaactttt ccctttccgc
     3541 cagttcggcg ggctttattg ttaccgccgc gcagttgggc tatgccgcag gtctgctgtt
     3601 tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact
     3661 ggcagcgggc ggtatgttaa tcactgccag cagtcagtca ctggcgatga tgatcctcgg
     3721 tacggcatta accggtttat tctcggtcgt ggcacaaatt ctggttccac tggcagcgac
     3781 gctggcctca ccggacaaac gcggcaaagt ggttggcacc attatgagcg gcctgctatt
     3841 ggggatctta ctcgcacgga ccgttgccgg attgctggca aacctcggcg gctggcgcac
     3901 cgtcttttgg gttgcctcgg tgttaatggc actgatggcg ctggcattat ggcgtggtct
     3961 gccacaaatg aaatcagaaa cccacctcaa ctacccacag ttgcttggtt ccgttttcag
     4021 catgtttatc agcgataaga tcctgcgcac ccgcgcgttg ctgggctgcc tgacctttgc
     4081 caacttcagc attctctgga cctcaatggc ctttttgctt gccgctccac cttttaacta
     4141 cagcgatggt gtaattggtc tgtttggact tgcgggagct gccggagcgt tgggcgctcg
     4201 tccggcgggc ggttttgccg acaagggcaa atcgcaccac acaacaactt tcggtctgct
     4261 tctgctgtta ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgctgat
     4321 tatcggcatc ctggtgctgg acctcaccgt gcagggcgtg catatcacta accagacggt
     4381 aatttatcga atacaccctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta
     4441 ctttattggc ggtgccgctg gttcgctaat ttcagcctca gcctggcaac atggcagttg
     4501 ggctggcgtg tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg
     4561 aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac
     4621 acttataagg gtgttaagaa gcccatcagt ctgataa
//
LOCUS       DAJOTB010000251         4646 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJOTB010000251 DAJOTB010000000
VERSION     DAJOTB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.251"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            22..1683
                     /gene="recN"
                     /locus_tag="OHN28_004708"
     CDS             22..1683
                     /gene="recN"
                     /locus_tag="OHN28_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCQ2299967.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            1832..2173
                     /gene="bamE"
                     /locus_tag="OHN28_004709"
     CDS             1832..2173
                     /gene="bamE"
                     /locus_tag="OHN28_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCQ2299968.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(2235..2525)
                     /locus_tag="OHN28_004710"
     CDS             complement(2235..2525)
                     /locus_tag="OHN28_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCQ2299969.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2515..2952)
                     /gene="ratA"
                     /locus_tag="OHN28_004711"
     CDS             complement(2515..2952)
                     /gene="ratA"
                     /locus_tag="OHN28_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCQ2299970.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            3123..3605
                     /gene="smpB"
                     /locus_tag="OHN28_004712"
     CDS             3123..3605
                     /gene="smpB"
                     /locus_tag="OHN28_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCQ2299971.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3820..4182
                     /gene="ssrA"
                     /locus_tag="OHN28_004713"
     tmRNA           3820..4182
                     /gene="ssrA"
                     /locus_tag="OHN28_004713"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1292 a         1177 c         1098 g         1079 t
ORIGIN      
        1 ttttcataca ggaaaacaac tatgttggca caactgacca tcagcaactt tgctatcgtt
       61 cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga gaccggcgcg
      121 ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc tgaagccgac
      181 atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct gaaagatacg
      241 ccagcggccc tgcgttggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt
      301 cgtcgcgtaa tcagcagcga tggtcgctcc cgtggtttca tcaacggtac agctgttcct
      361 ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat
      421 cagttactca ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc
      481 tctcagctcc aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc
      541 gcgcatcatc agcagttaag tcaggaacgc gccgcccgtg cggagctgct gcaataccaa
      601 ttaaaagaac ttaacgaatt taatccgcag cctggagagt ttgagcaaat cgacgaagag
      661 tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta
      721 atggctgacg gtgaagacgc aaatctgcaa agtcaacttt acacggctaa acaactggtg
      781 agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaggct
      841 accatccaga ttgctgaagc cagtgatgaa ctacgccact actgcgatcg tctggatctc
      901 gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt
      961 aaacatcacg tcagccctga ggcattgcca cagtattacc agtcgctact ggaagaacag
     1021 cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat
     1081 catcagcagg cactggaaac ggcgcgcgcg ttacaccagc aacgccagca ttatgcaaat
     1141 gagttggcac agctgatcac cgatagtatg catgcactct ctatgccaca tgggcagttt
     1201 acgatcgatg ttaaatttga cgagcatcac ctgggcgctg acggcgctga tcgtattgag
     1261 tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt
     1321 ggtgagttgt cccgcatcgc attggcaatc caggtcatta ccgcgcgtaa aatggaaacc
     1381 ccagcactga tttttgatga agtggatgta gggattagcg gcccaacagc ggcagttgtc
     1441 ggcaaactgc tacgtcaact tggcgaatca actcaggtga tgtgtgttac ccacctgcca
     1501 caagtcgcgg gatatggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg
     1561 acagaaacgc atatgcaatc cctggataaa aaagcgcggt tacaagagtt ggcgcgcctg
     1621 cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcggcg
     1681 taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc
     1741 aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta
     1801 ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc
     1861 agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc
     1921 tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat
     1981 gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac
     2041 aaatacctgg ttctatgtct tccgccagca acctggtcat gaaggtgtaa cgcagcaaac
     2101 gctgacgctg acctttaaca gtagcggtgt gttgaccaac attgataaca aacctgcgct
     2161 gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac
     2221 cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc
     2281 ggatcggcaa tgagaggacg ataaatctcc acccgatcgc catcatgcac aatatcgctg
     2341 agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc
     2401 aattccagca aaccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcaacgtc
     2461 acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct
     2521 ggcactgtag acctcttttg cacgaacagt aaaagcctgc accatattag ctgccagctc
     2581 tttaaacaca cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg
     2641 aaactcaata cggcacgcgt cctggctcaa cggcgtaaac ttccaaccgc ctatcaattt
     2701 cttaaacggt ccatcaacca gactcatgag aatactttga ttactggtca actggttgcg
     2761 ggttgtaaac gttttgctga tcccagcctt agacacatct accgcagcgg tcatctgccc
     2821 aggagtggac tccagaatcc ggcttccagt gcaccctggt aaaaactgag gataagactg
     2881 aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct
     2941 aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc
     3001 tgtaaaaaaa acgctatccc ggcgctgggt aacatcggga tcatgctaag atagagcctt
     3061 gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc
     3121 ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg
     3181 cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg
     3241 aggttaaatc cctgcgtgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg
     3301 acggagaggc atttctgttt ggcgctaaca tcacgccaat ggctgtggcc tccacgcatg
     3361 tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat
     3421 tgtacggtcg cgtaaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa
     3481 atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt
     3541 cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa aacgcccatc
     3601 gttaaaccta tataagcgat actaatcaaa tcctcaccgc gcctccctat ccggtggcgc
     3661 gaatgaacat cttgtcgact gtcaaatctg acacagatgt agccatccca ttgcttaatc
     3721 gaaaaaaaat catactatgc aggcgctaat tccaggatga taacgccttt tgggctagtc
     3781 atgctgctca taaatctggt atacttaccg ttacacattg gggctgattc tggattcgac
     3841 gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa
     3901 aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc
     3961 tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc
     4021 gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg
     4081 tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg
     4141 taggaatttc ggacgcgggt tcaactcccg ccagctccac caaataatga ttggacggtg
     4201 taaggactac accaacaaaa acaggaagtt ataagtctca gcaggacacc gaccagacgg
     4261 tgaggagaca aaaaaggata cgcaacggag tcgcgactcc ctatgacatt aaagcccgct
     4321 gatgcgggct tttttattgg tcctgacccg ccctgaatag aattcatcca attacagtcc
     4381 agacatatac agtatccctc agctcagtat cagcttctcg acaaatggtc actgttattt
     4441 acccgtttcc agaaagagaa taatttgccc ccatagccag ccttcccgga atctgtatgt
     4501 tcattaatat tgttatcgat gccatcaagt aacataggtt ggatgcgatt gatcttactc
     4561 acattagtcg acacggaact cagtaagttg aactctgaaa aagcagttta aatagcattt
     4621 aaacaaatgc caccagttcg aaaaag
//
LOCUS       DAJOTB010000252         4607 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJOTB010000252 DAJOTB010000000
VERSION     DAJOTB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.252"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..460)
                     /locus_tag="OHN28_004714"
     CDS             complement(<1..460)
                     /locus_tag="OHN28_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCQ2299972.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVT
                     LPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYV
                     YPALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISN"
     gene            834..>4607
                     /locus_tag="OHN28_004715"
     CDS             834..>4607
                     /locus_tag="OHN28_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2299973.1"
                     /translation="MCSKRNIGDMKITVLNQRYKKHFGYPHILMGDVKVTVSAMIACA
                     VLLHPNKLFASVAGTSLPYQTYRDFAENKGEFRPGAENVPLYDKNGNPVTTLNKAPMI
                     DFSSNDSTGVATLVSPQYVVSVKHNVGYQYVKFGYADDSSYALVDRNNHWRDFHTPRL
                     NKIVTEVTPLDITNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGAYSYK
                     TGGIVNTPAISDWSFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLNSYAG
                     VNGNTNWYTIIPAGDVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGSTSWE
                     MHGNLGATWPASLNSGKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQTWKG
                     GGIIVNGEHLVDWQINGVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQRADD
                     NGLSQAFSSVSIVSGRPTLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLNGADD
                     GAILTNSGSMANVNLDFNSPDTTATIANIWHGHFTGNLNINNEVTAGTQNDFAIDGGV
                     NAQGSITQQNGRLFMQGHPVVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWENRQF
                     SMAELNLQNAEFNLARNASLNTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTLGKSK
                     ATAEDDQSRFNGHVQLQQGSALTINEHFAGGIDSADSATTITSTDTTLNQLSRFTQSS
                     LSLGEGAKLTATAGLLSDGTVSSNAGASLSLLSDQPGTMYSAQSWELSGQDTSLNVGA
                     GGIITGDINANDAASISFGTTDINQSTNYYGNINAPLASVTMKDTAWQVNKQSVAKSL
                     TLNGSTLSFNRFGQGGLTSDTLEATNSSFIINADGKAADTVTVNQALTGGNNTLVVIP
                     TTNSVKQGGDPVSLVTAPKNTQSNIFTLNPVSINAGFHSFTPQLDVLETDVNKQWRLE
                     GFYIQPDKAALRTGKSFMDLGYKNFITEINNLNDRMGDLRHTHGETGAWARLNSGSGS
                     ATDGFTGSYTHLQIGADRKHIIEGGELFTGVTATFTSSNNRGTGWSGRTKSTGIGVYA
                     SAMFDSGLYVDTIGKYVRHDNHYSSSALGMPEQDYGSHSWYLGAEAGWRFSLPDETYI
                     QPQTELIYGTVSENQFAWQFNGGEIYMQRKQMQPLIGRTGIEFGKTFRGKDWEMTALT
                     GINYQYDLFKPTVTAFKDLAGDTYINNGKDSRVVFNVGVNTKIKENTRISLNVE"
BASE COUNT         1402 a          891 c         1071 g         1243 t
ORIGIN      
        1 tatttgatat tttaatcatt tgcaactcga ttggatatgt accattagca agttcgattg
       61 gtgcattata agaagataca cctttatcac cgactttcat ccaaagcgca aaatcctttt
      121 gttgattacc ggtagcctcg tttagagcag gataaacata gccattagac tggaaattag
      181 tagttgctac cgttagtgat gcaggagtgg tacaatcggt aacactaatc ccctgatctg
      241 cggttacact ttttattttc tggtacgctt ttgcagcatt tacctcttga ttcgttactg
      301 tgccaaaaga tgctgttgca ttaggcaggg taacactgca agtcgcggca ttaaaagaac
      361 cagtagcgga gacagaacca ctgcttgtct gggccattgc ggtaccagaa ataagcagag
      421 ccgaaatagc tgcgatggtg attttattct tagaaaacat tttcaacctc aatcttgatt
      481 ttcttaaatt tttcctatgt gtatagtgat gggcaaagag atgtgagaat aaaactttca
      541 aaaattagca acacatccca atgcctcttt tctattcttc aaaaaaaaaa gattaattca
      601 agtcataaaa atacaaccat ttgatactta tttgtacatg acgggatttt tgctgaattg
      661 tcattcctta agtacaaaat agcaattcta aaaaatagaa tattttgata ataaacaata
      721 aaatatatgc gtgagggttg actctgagca atgcttggat ttattagttt taagatcttt
      781 cgagtcagaa gatatatgaa tagatagaat agaaattcaa ttgtcggaat gaaatgtgtt
      841 caaaaagaaa tataggtgat atgaaaatta cagtccttaa tcagcgatat aaaaaacatt
      901 ttggttatcc tcatattctg atgggtgatg taaaagtaac tgtttcagca atgattgctt
      961 gcgctgtttt attgcaccca aataaattgt ttgcctccgt cgctggaacc tcacttccct
     1021 atcaaacgta tcgtgatttt gctgagaata agggagagtt ccgtcctggc gcagaaaatg
     1081 ttcctctata tgataaaaat ggcaatcccg tcacgacgct gaataaggca cccatgattg
     1141 acttcagctc taatgactcg actggggttg caacactggt ttcgccacag tatgtcgtca
     1201 gcgttaaaca caatgttggt tatcaatatg tgaaatttgg ctatgcagat gattcatcct
     1261 atgcccttgt tgacagaaat aaccactgga gagatttcca cactccccgt ctcaacaaaa
     1321 ttgtcaccga ggttacacct ctggatataa ccaatgcggg tacagctaag ggaacctatc
     1381 aaaatgctga ccgtttccct gtgttctatc gagtaggtac gggaactcaa tatgtaaaag
     1441 atactaacgg aaaaatcacc tatttaatgg gagcctactc gtataaaaca ggtggaatag
     1501 taaatacgcc ggctatatct gactggagtt ttgtcacgaa taccactaat actccactct
     1561 caacctacgg cactcccggt gacagtggct ctcctctttt tgcctgggat gcagaccaaa
     1621 ataaatgggt tttgttagcc gttctcaatt catatgcagg ggtcaatggc aatactaact
     1681 ggtacacaat aattcctgct ggtgatgtaa aaaatacgat gaaacaagat gccgatgcac
     1741 cagtaaatac aaaacaaggt gaaggggata ttcattggtc atatgacgaa aaaacgggac
     1801 ttggttcatt aacccaggga agcacatcat gggaaatgca tggaaatttg ggggcaacat
     1861 ggcctgcttc gcttaatagt ggtaaagatc taacttttca gggcggcggt actgtagtgt
     1921 tagaaaatac cgttaatcag ggggctggca cacttacttt tgatgatgat tatattgtca
     1981 agcccgttga tactcagaca tggaaaggcg gcggaattat tgttaatggt gaacatcttg
     2041 ttgactggca gattaatggg gttactggtg atagtttgca taaactgggt acgggtacgt
     2101 taaaaattaa cggtaccggt gtaaacccag gaagcttaag tgtgggagac ggaaccgtta
     2161 tattggcaca gcgtgctgat gataacggac tgagccaggc gttcagttct gtcagtattg
     2221 taagtgggcg tcctactctg gtgctaaacg acgacaaaca aattaatccg gataatatca
     2281 aatggggcta tcatggcggc aagctcgaca ttaatggaaa cagtttgact tttcataaac
     2341 ttaacggggc tgatgatgga gctatactga ctaacagtgg ctccatggct aatgttaatc
     2401 tggatttcaa cagcccagat acaacagcca cgattgctaa tatctggcac ggccatttca
     2461 ccggtaatct aaatatcaat aatgaggtta ccgcaggaac acagaatgat ttcgctattg
     2521 atggtggagt gaatgctcag gggagtataa cccagcaaaa tggtcggcta tttatgcaag
     2581 gacatccggt tgttcacgcc gtcagcagtc aggacgtagc aaataagcta aaggcgctgg
     2641 gtgacaattc agtactgacc caaccagtgt catttactca gaacgattgg gaaaatcgac
     2701 agttcagtat ggcggagctt aacttacaaa atgctgaatt taaccttgca agaaacgcca
     2761 gccttaatac cagaattaat gccgagcatt ctaccgtgac gttaggtagt gaggatcttt
     2821 acattgatct caatgacggg aatggcgtgg caaccaaacc aacattgggt aagtcaaagg
     2881 caaccgctga agatgatcag agtcgtttca atggacatgt acagttgcaa caaggatcgg
     2941 cactaactat taatgaacat tttgccggcg gtattgatag tgcagatagt gcgacgacga
     3001 taacctctac agataccacg cttaatcagc tcagccgttt cacccaatct tctttgtcat
     3061 taggtgaagg ggcaaaattg accgctacag ccggattact ttctgacggg acggtaagca
     3121 gtaatgccgg ggcgagcctt tccttgctat cagaccagcc tggaacgatg tattccgccc
     3181 aaagctggga gcttagcggg caagacacga gcttaaatgt tggcgcgggt gggatcatca
     3241 ctggagatat caatgccaat gatgcggcca gcattagctt tggcacgact gatattaacc
     3301 aatccacaaa ttactatgga aatatcaacg cgccgttggc aagtgtaaca atgaaggaca
     3361 ctgcctggca ggtaaacaag cagtcggtgg ctaagtcgct cactttgaat ggttcaacac
     3421 tgagctttaa tcgtttcggg caagggggac tgacgtcaga tacgctggag gccactaaca
     3481 gttcgttcat tatcaatgcc gatggtaaag cagcagatac ggtgacggta aaccaggcat
     3541 tgacaggggg gaataacaca ctggttgtta ttcctacgac aaattcagta aaacagggcg
     3601 gtgaccctgt gtcattggtt actgccccga aaaatacaca aagcaatata tttacactta
     3661 acccggtttc aataaatgca gggttccata gttttacacc gcaacttgat gtgttggaga
     3721 ccgacgttaa taagcaatgg cgtctggagg gattttacat acagcctgat aaagccgctt
     3781 tgcggacagg caagagtttt atggatctcg gttataaaaa ctttattaca gaaattaata
     3841 atctgaatga tcgtatgggg gacttacgcc atacccatgg tgaaactggg gcatgggcta
     3901 gacttaacag tggttcgggc tcagctactg atggatttac cggaagttat acgcacttgc
     3961 aaattggggc agacaggaaa catataattg aaggcggaga gttatttact ggagtaacgg
     4021 cgacatttac aagcagtaat aaccggggaa ctggttggtc gggaagaacc aaatcaacgg
     4081 gtattggtgt gtacgcttca gctatgtttg actctggact ctatgtagat actatcggga
     4141 aatatgtgcg tcatgacaat cattactcat caagtgcgtt aggaatgcca gaacaagatt
     4201 atggctcgca ctcctggtat ttgggcgctg aggccggttg gcgtttttcc ttacctgacg
     4261 aaacatatat acagccgcaa acagagttga tctacggtac tgtatctgag aatcagtttg
     4321 cctggcaatt caatgggggt gagatctata tgcaacgtaa gcagatgcaa ccattgattg
     4381 gtcgcacagg aattgaattt ggcaaaacat tccgcggcaa agactgggaa atgacagcgc
     4441 tgactgggat taattatcag tacgatttat ttaagcctac tgtgacagcg ttcaaggatc
     4501 tggcaggtga tacatatatt aataatggta aagatagcag agtggtattc aatgtcgggg
     4561 taaatactaa aattaaagaa aacactagga taagtctgaa tgtggag
//
LOCUS       DAJOTB010000253         4508 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJOTB010000253 DAJOTB010000000
VERSION     DAJOTB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.254"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            602..928
                     /locus_tag="OHN28_004716"
     CDS             602..928
                     /locus_tag="OHN28_004716"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2299974.1"
                     /translation="MLIAAVPALAESAHTIDPARIKLYSETVRVWNQSRGSAGARALA
                     DMLIQNGIPMSSYRARRLMKHLNLRSAQPGKYQYKNARQEHTSLPSLLECRFSVLEAE
                     QVWILK"
     gene            complement(1602..2531)
                     /locus_tag="OHN28_004717"
     CDS             complement(1602..2531)
                     /locus_tag="OHN28_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2299975.1"
                     /translation="MNSIFTEENLLAFTMAARYRSFSKAAKELGLTTSAISYTIKRME
                     MGLDVVLFTRTTRSIELTEPGHYLFCKATDLLNDYHSIIRRVDAIAQGIKSRVRICIN
                     QLLYTPQHTARLLQVLREHFPTCQIAITTEVYNGVWDSLINNQADIAIGAPDTLLDGG
                     GIDYIEIGMIRWLFAISPAHPLAFIPEPISESQLRLYPNIMVEDTANTINKKVGWLLH
                     GQESILVPDFNTKLQCQILGEGIGFLPDYMVREAMEKSLLVTRQIHNPRQDSRMLLAT
                     RHSASGQVTQWIKREFSLQGILTGIYQDLMHRA"
     gene            2919..3524
                     /locus_tag="OHN28_004718"
     CDS             2919..3524
                     /locus_tag="OHN28_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCQ2299976.1"
                     /translation="MSNKELITEFLLAAEQGNSDGLKSCLDKKVDINATNREGRTAII
                     IASLNKHYDCVSLLISAGADINKQDQTCFNPFLIGCLNNDLTLLRTVLPANPDLNCLT
                     RFGGVGITPACEKGHVEIVRELLMRTDINVNHTNFVGWTPLLEAIVLNDGGEKQQEIV
                     KLLLEHGANPHMTDKYGKTPLELAREKGYHKIAELLISAGA"
     gene            3610..4458
                     /locus_tag="OHN28_004719"
     CDS             3610..4458
                     /locus_tag="OHN28_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2299977.1"
                     /translation="MGQVEQVFSHAVNLLIPEQQRLLTLLSEDCDNAPNSCRLALTHY
                     EDLFHPGEQVNFSGSGITVGNDKWIEITGCQMWRIPRWTSDSASLAKVGWQHWEEFIR
                     QQLRSNDTLFLYQGDNPFYQEISRQLLNRRKNLLKALCTGENITAAVNQLIGLGIGLT
                     PSSDDYLVGLSTILFIQGHPLRKYRYDFLAALQLAGNNITLLSKITLEEAFNQRYRER
                     VGRLLNQFITEPQPISTQYVTDIKNIGSSSGCDMLYGMADACALSHRSGGNYVNQDSC
                     KKKHVF"
BASE COUNT         1266 a          999 c         1013 g         1230 t
ORIGIN      
        1 gggatgccag cctggcgact ataactccga aaagaccgat tattgtattg cgtgcatccg
       61 gcatgtttac aatgtacgct ggtatctaac ggagtctgac acagatgcac gcaggtgtct
      121 attttaacgt aattatccgg aaagaacagt atattttaat gcgggagttg catgagcggt
      181 atacgggaga aactatcaga ccatgggagc tacagaggtg cttaagtctc gtaacattat
      241 aatctgctca cctgactggt gctattctca cctcaacacc aatgcacaaa tagcctggct
      301 tatcgttaat aaaagttact cataccggga atccagtgaa gctatgaatg tcggttccta
      361 tacactgggg gcatgggtac gtcagctcag gcgggaacgt caggatacca cgtcttctgc
      421 atcaccactc acatcagagt agcaacgtat tcatgagctg gaaaagcagg ttcgtcgcct
      481 ggaggaacaa aaaacgatat taaaaagcta cagtgctctt gatatcctcc ccactgaacg
      541 gtttacgata gttaccagac tgagcggcga cacacggttg tcagtctttg cgctgctctg
      601 gatgctcatt gcagcagttc cggcactggc agaatcggct catacgattg atccggcgcg
      661 aatcaaattg tatagcgaaa cagtccgggt ctggaaccag agtcggggtt ccgcaggtgc
      721 gcgtgctctg gcagatatgc tgattcaaaa tggtatcccg atgagcagtt atcgtgccag
      781 gaggctgatg aagcatctga acctgagaag tgctcagccc ggaaaatatc agtacaaaaa
      841 tgcccgccag gagcatacca gcctgccgag tctgcttgag tgtcggtttt ctgtgctgga
      901 ggccgagcag gtatggattc ttaaataaac ctcgagtctg aatcggtaac cagctgttat
      961 attctgaatt attacaatag cgtcaggcct cataattaca ctgtgggttg acgccggaag
     1021 aatcagagaa cagataccgt tcttactgta aaacgtggct aatattaatt gagcactgca
     1081 gtgttcagag cacaaagaag aaaaaagatg tgtgcgaatg cctgtaatgt gttaaaagaa
     1141 tgttccgcgt ttccggtacg cttttcgtaa ctaacaatgc agggtgacgt aacgctggtt
     1201 gtgccggaaa cgcgtccctc cccctatctt tcctgtttgg atttgcccct atatttctgg
     1261 attatccgac aaaaccggcg tgaagtatta ctctcgtcgc ccagcaccat aaggataacg
     1321 cccataaaat catgaaactt ttcttccaga ttggatacaa tcaggccgaa acggtgatga
     1381 atattatcca aatctgatgg ttcgtagcgt tttcagcccc cagattctga gttgcctgta
     1441 gggatgattt caattcccga tttgtctatc gcatagagaa aaagaatttc atttctgttc
     1501 accagaaccc atgtaaccca ctccaattcg ttatttatct cactgattgc ccttcattta
     1561 tctcattgat gcaccgaaag gtaaaactgc catccggtgc atcacgctcg atgcatcaaa
     1621 tcctgataaa tccccgtcag tattccttgt aagctaaatt ctctttttat ccactgagtg
     1681 acttgaccgc ttgccgaatg cctggttgcc aacagcattc gtgaatcctg acgtgggttg
     1741 tggatctgcc gtgtcaccag caatgacttt tccatcgctt cacgcaccat atagtctggc
     1801 agaaaaccga ttccctcccc caagatctgg cactgaagct tagtattaaa gtcgggaacc
     1861 agaatcgact cttgcccgtg taacagccag ccgacttttt tgtttatagt gtttgcagtg
     1921 tcttccacca tgatgttcgg gtacagacgt aactgactct cagaaatggg ctcaggaata
     1981 aacgccagcg gatgagccgg agaaatcgcg aacaaccaac gaatcatgcc gatttcaatg
     2041 taatcaattc caccaccgtc cagtaacgta tcgggcgcgc caatggcgat atcagcctgg
     2101 ttattaatga gtgaatccca gacgccatta tacacctctg tagtaatagc tatctggcag
     2161 gttggaaagt gttccctgag cacttgcaga agccgagctg tatgctgagg ggtatacaac
     2221 agttgattaa tacaaatgcg cacccgggat ttaattccct gagcgatggc atcaactcta
     2281 cgtatgatgg agtggtaatc gttgagcagg tcagttgctt tgcaaaaaag gtagtgcccg
     2341 ggctcggtca gctcaatgct acgggtagtt cgggtaaaaa gcaccacgtc aagccccatt
     2401 tccatacgct tgatagtgta actaattgcc gatgtggtta agcccagttc cttcgccgcc
     2461 ttgctgaaac ttctatagcg ggcagccata gtaaatgcca gtaagttttc ttccgtaaat
     2521 attgaattca tctttctttc ctctggcgac aatacacata cacaacatga tgattgttga
     2581 atgtatttgt aattcttata accgacaatc agttttgaaa aaactaacac cgtatttaca
     2641 tttaatttga aggaaatcac acatctgaat tttaattaca ggcagctatc cgtgctgcag
     2701 accaccttta tttatatgac tgcggattaa tggctaaaac atccccagct ataatagaaa
     2761 tattgcacta tatcacactc tcttattatt gttaattttg tttcaataat tcatggtcta
     2821 atgatgagta aattttaaat ggatggtgct ctatgcattc tgttgctatt cttggcttgt
     2881 tatatttgta aaatcttaaa aagagcagga gatcgattat gtccaataaa gaactcatta
     2941 cagaatttct gctggcggca gaacaaggta attccgatgg attaaaatca tgccttgata
     3001 aaaaggttga tattaatgcg actaaccgcg aggggcgtac tgcaattatt attgccagtc
     3061 tgaataaaca ttatgactgc gtttctttat tgatttccgc aggtgctgat attaataaac
     3121 aagatcaaac ttgttttaat cccttcctga ttggctgcct gaataatgat ttaacactgt
     3181 tgcgtacggt attaccggct aaccccgacc ttaattgcct aacccgtttt ggcggtgttg
     3241 gtattacacc tgcctgcgaa aaaggccatg tcgagattgt acgtgaattg ctgatgcgta
     3301 ctgatatcaa tgtcaaccat actaattttg ttggctggac gccgctccta gaggccatcg
     3361 tgctgaatga cggcggtgaa aagcagcagg agatcgtcaa actgttgctt gagcatggcg
     3421 caaacccgca catgacggat aaatacggta aaaccccgct ggagctggcg cgagaaaaag
     3481 gctaccacaa aattgccgag ttgctgattt cggctggagc ctgacaacag gagcacgatt
     3541 atcgctatat tatcacggca atcttttcgg gcactaaccg cagactgcgc gtttctcact
     3601 tttcggggaa tgggccaggt tgaacaggtt ttttcccatg cggtaaatct gttaatacct
     3661 gaacagcagc gactgctgac gttactgagt gaggattgtg ataacgcacc taacagttgt
     3721 cgtttagcac tgacacatta tgaagacctt tttcaccccg gtgaacaggt caattttagc
     3781 gggtctggaa taactgttgg aaatgataaa tggatagaaa taaccggttg tcagatgtgg
     3841 cgaataccac ggtggacatc tgattcagca agccttgcca aagtcggctg gcagcattgg
     3901 gaagagttta tccgccaaca actaagaagc aacgacacat tatttctgta ccagggtgac
     3961 aatccatttt atcaggaaat ttctcgccaa ttactcaacc gccgaaaaaa tttgctgaag
     4021 gcgttgtgta caggagaaaa catcactgcg gcagtcaacc agttaattgg tcttggaata
     4081 gggctgacgc catcatcgga tgattattta gtaggtttaa gcactatttt atttattcaa
     4141 ggacatccac taaggaagta tagatacgat ttcttagcag cattacaact tgcaggaaat
     4201 aacataacgc ttctcagcaa aataacgtta gaggaagcat ttaatcagcg ttatcgggaa
     4261 agagtcggac ggctactgaa ccaatttatt actgagccac aacccatttc tacgcaatat
     4321 gttactgaca taaaaaatat aggctcaagt tctggatgcg acatgctgta tggcatggcg
     4381 gatgcctgcg ccctgagcca caggtccgga ggtaattatg tcaatcagga tagttgtaaa
     4441 aaaaaacacg tattttgatt ctgtttcact gatgtctatc tccacgcgcg caaataagct
     4501 tgatggag
//
LOCUS       DAJOTB010000254         4468 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJOTB010000254 DAJOTB010000000
VERSION     DAJOTB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.255"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            595..1092
                     /locus_tag="OHN28_004720"
     CDS             595..1092
                     /locus_tag="OHN28_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2299978.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSETWRDKQTGEMREQTEWHRV
                     VLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTMQMLGRAA
                     GAQTQLEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPEPQLQPPEGDDYGFS
                     DDIPF"
     gene            1155..1388
                     /locus_tag="OHN28_004721"
     CDS             1155..1388
                     /locus_tag="OHN28_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCQ2299979.1"
                     /translation="MREYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1447..3405
                     /locus_tag="OHN28_004722"
                     /pseudo
     CDS             1447..3405
                     /locus_tag="OHN28_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3460..3894
                     /gene="psiB"
                     /locus_tag="OHN28_004723"
     CDS             3460..3894
                     /gene="psiB"
                     /locus_tag="OHN28_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCQ2299980.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWGLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYGVNDIIHILMAEGGQV"
     gene            3891..>4468
                     /locus_tag="OHN28_004724"
     CDS             3891..>4468
                     /locus_tag="OHN28_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCQ2299981.1"
                     /translation="MSARSRALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FCCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLA"
BASE COUNT         1070 a         1170 c         1389 g          839 t
ORIGIN      
        1 caaatcgcta cggcaaaata atggagatct ggctattacc gttcaggcca aaaatggatc
       61 ccgaaagatt gaggttttca aactcaaaca tgtcaatcgc aaccgaacaa cgattgtcga
      121 tacggagccg agaaccgcac atatcacaac ccgcacaaca gagatcatgc gtcgattagg
      181 ggcaaggatc tgcgagtatt gtgcgaagac tgggcgctgc gaagtacatc atgtcagaaa
      241 gctaaaagac ctgaagaaag gtcgtaaggc cggatataaa cccagcctgt ggcaactaat
      301 gatgatcgca cgccgccgga aaacgatgat tttgtgcgca agttgccacg acaagttaca
      361 tagcggaaaa ctacccgatc tgagacagga aaagggaaga atactcctgc aaccacctgt
      421 ctcaagcggg gattctgcta aatgagttta tccccaataa gctagcaatg gagagccgta
      481 tgcattgaaa ggtgcacgta cggttcggag ggggggtaac ggtgatctaa ctcaagataa
      541 gcaggacata ttcgccattt atcttgaaag accacacgta ccctacccta ccgcctggga
      601 aaagatccgg aagtccgtta catccccaac gggggcgcag tggcaaacct gcaggtcgcc
      661 acgtcggaaa cctggcgtga caaacagaca ggggagatgc gggagcagac agaatggcat
      721 cgcgtggtgc tgttcggcaa gctcgcggaa gtggctggcg aatatctgcg caagggcgtg
      781 caggtctaca tcgaaggtca gcttcgcacc cgtagctggg aagataacgg tatcacccgt
      841 tacgtcaccg aaattcttgt taagaccaca ggcaccatgc agatgctggg acgtgccgca
      901 ggtgctcaga ctcagctgga agaggggcaa cagttcagcg gtcagcctca gccggaacca
      961 caggcggagg ccggtacgaa aaaaggtggc gcaaaaacga aaggccgtgg acgtaaggcc
     1021 gcgcagccgg agcctcagct gcaaccgccg gagggtgacg attacgggtt ttcagacgat
     1081 atcccgttct gaacgggctg actgtgataa ccgccccgcc ccgttctgtg cggggcatca
     1141 ccggagagat gaggatgaga gaatatttca gaatacttca ggggctgccg gacggctcct
     1201 ttacccgcga acaggcggaa gccgttgccg cacagtaccg gaacgtcttt atcgaggatg
     1261 accacgggga acattttcgc ctggttgtcc gcaataacgg tgcaatggtc tggcgtacct
     1321 ggaattttga ggacggtgcc gggtactgga tgaaccatgc catcagggat ttcgggattc
     1381 ttaagtaaga gaggtgccgg acgcgcgaac gtccggcagg acataagcaa ttataagggg
     1441 atgattatgc ctgtaacgaa gtgtgaacca gaaaccaccc gcaaagcaag ccgtaaatat
     1501 gcaaaaacgc aggaaacagt cctgtctgcc ctgctggcgc agacggagga agtgagcgtg
     1561 ccgctggcct cactgattaa atcaccgctg aatgtgcgca cggtgccgta ttctgcggag
     1621 tccgtcagcg aactggcgga ctccattaaa tgtgtcggcc tgttacagaa tctggttgtt
     1681 catgccctgc caggtgaccg ttacggtgtc gccgcaggcg gtcgccgact ggcagcactc
     1741 aacatgctgg cagagcgcaa catccttccg gctgactggc ctgtacgcgt gaaggtcatt
     1801 ccgcaggagc tggcgactgc cgcatcgatg accgagaacg gtcagcgtcg ggatatgcac
     1861 cctgccgaac agattgccgg attccgcgca atggcgtagg aaggcaaaac acctgcgcag
     1921 actggcgacc tgctgggcta ttcaccccgc cacgtccagc gaatgctgaa actggcagac
     1981 cttgcacccg tcatcctcga tgcgctggca gaagaccgca tcaccacaga gcactgtcag
     2041 gcgctggcgc tggagaacga caccgcgcgt caggtacagg tgtttgaagc cgcctgtcag
     2101 tcgggatggg gcggaaaacc ggaagtacag accattcgtc gtctggtgac cgaaagtgaa
     2161 gtggcggtgg cggggaacag taaattccgc ttcgtggggg ctgatgcctt ctcgccagac
     2221 gaactgcgca ccgatttgtt cagcgatgac ggggacggtt atgtcgaccg cgtggcgctc
     2281 gatgccgccc tgctggaaaa actccaggct gtcgctgaac accttcggga agccgaaggc
     2341 tgggaatggt gcgccggacg catggagcct gtcggtttct gccgtgagga tgccggaaca
     2401 taccgcagtc tgccggagcc ggaagcggtg ctgacggagg cagaagaaga acgcctgaac
     2461 gaactgatgg cacgttacga cgcgctggaa aaccagtgtg aggaatctga cctgctggaa
     2521 gcagaaatga agctgattga ctgcatggcg aaggtcaggg cgtggacgcc ggaaatgcgt
     2581 gccggaagcg gtgtggtggt gtcctggcgt tatggcaacg tatgtgtcca gcgtggtgtg
     2641 cagttgcgca gtgaagatga cgctgctgac gacgctgacc gcacggaaca ggtgcaggag
     2701 aaagcgtcag tggaggaaat cagtctgccg ttgctgacga aaatgtcctc agagcgcacg
     2761 ctggcagtcc aggcggcact gatgcagcag ccggacaaat ctctggcact gctggcatgg
     2821 acgctctgcc tgaatgtgtt tggcagcggg gcgtacagta aaccagcaca aatcagcctg
     2881 gaatgtgaac attattcgct gaccagcgat gcgccatcgg ggaaggaagg tgccgcattc
     2941 atggcgctga tggcagaaaa agcccgtctt gctgccctgt taccggaggg atggtcacgg
     3001 gacatgacga cattcctgtc actcagtcag gaggtgctgt tatccctgct cagtttctgc
     3061 accgcatgca gccttaacgg tgtccagacc cgtgtgtgtg gtcacacgtc acgcagtccg
     3121 cttgactcgc tggaaagcgc tatcggattc cacatgcgcg actggtggca gccgacaaaa
     3181 gcaaacttct tcggacacct gaaaaagccg cagattatca cagccctgaa tgaggcagga
     3241 ctgtccggtg ccgcacggga cgcggagaag atgaagaaag gtgatgcggc tgaacatgca
     3301 gagcaccata tgaaagacaa ccgctgggtt ccaggctgga tgtgtgcacc acatccacag
     3361 acagatgcca ctgaacgcac cgataacctg gctgatgccg tctgatgaac aaccacaccg
     3421 ccccgccgga gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg
     3481 aatgccctgc agtccatgaa cgcacaggaa tatgaagata tccgtgctgc gggaagcgat
     3541 atgcgccgta atctcactca cgaggtgatg cgtgaagtgg acgcaccggc taactggatg
     3601 atgaatggcg agtatggcag tgagttcggg ggctttttcc ccgtccaggt tcgtttcacg
     3661 ccagcccacg aacgtttcca cctggcatta tgttcgccgg gagacgtctc tcaggtctgg
     3721 gggctggttc tggtgaatgc cggtggtgag ccgtttgcgg tggttcaggt gcagcggcgg
     3781 tttgcaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga tacgcaggga
     3841 tacggcgtta acgacatcat ccatatcctg atggcggaag gaggtcaggt atgagcgcac
     3901 gttcacgggc actggttccc ctcagcacag agcaacaggc cgcatggcgg gcggtggcgg
     3961 agacagaaaa acgccgccat cagggtaaca cgctggcgga atatccgtat gcgggggcgt
     4021 ttttctgctg tctgaacgga agccgccgga tttcgctgtc tgacctgcga tttttcatgc
     4081 cgtccctgac cgcagaagaa ctgcacggca accgtcttca gtggctgtat gccatcgatg
     4141 tgctgattga aacacagggc gaggtctgcc tccttcccct tcccggtgat gcagcagagc
     4201 ggttgtttcc gtcggtgcgg tttcgtgtca gggagcgcag tcgccacaaa tccgcgctgg
     4261 tcatgcagaa gtacagccgt cagcaggcac gggaggcaga gcagaaagcc cgtgcgtacc
     4321 aggcactggt tgctcaggct gaaatcgaac tggcgttcca ttcaccggaa acagtgggaa
     4381 gctggcatgc acgctggagt gaccgggtgg cagagcatga cctggaaacc ctgttctggc
     4441 agtggggcga gcgttttccg tcactggc
//
LOCUS       DAJOTB010000255         4459 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJOTB010000255 DAJOTB010000000
VERSION     DAJOTB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.256"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1775
                     /locus_tag="OHN28_004725"
     CDS             <1..1775
                     /locus_tag="OHN28_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299982.1"
                     /translation="DSLKKAKDSITGLKEFAENIKPAKLRFDNVTKGYKKAQSEVDKI
                     AQQKAKAEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDVSSSISAMHRLSVAEQ
                     YKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRKAQLAPVKGGSGNSAT
                     MGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRARLGGVDVNQMYNITEW
                     AYKNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAELVKDKKTGKSEWKGGDNAINE
                     LLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDSQIGQRLEDLGDDLMLTSKYW
                     QRSVKDVQESINQQKASGKWLTETQQDSIVKFRELNRQLSQLSDARQVAFVDGFMKSL
                     DPKVTEEFMKNLSNLTPYFTKLGEATGSLFEAIMKIVNWFNRNDDKTDAIQKNMGDAP
                     PLSNDGMKQNLSNLVPDQYKGAGTATTTPDNSHSLFNTIKGLLFDDNSSVSDVKMSVN
                     EAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVVTEVPLTINSDTSRLSDYVDFT
                     ARASRDSFLKSLTLTSLSGQSNGG"
     gene            1811..2524
                     /locus_tag="OHN28_004726"
     CDS             1811..2524
                     /locus_tag="OHN28_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299983.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANTTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(2606..3883)
                     /locus_tag="OHN28_004727"
     CDS             complement(2606..3883)
                     /locus_tag="OHN28_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2299984.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     SLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMIALCLIGLYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKNYIELLRYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(4059..4217)
                     /locus_tag="OHN28_004728"
                     /pseudo
     CDS             complement(4059..4217)
                     /locus_tag="OHN28_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4266..4451
                     /locus_tag="OHN28_004729"
     CDS             4266..4451
                     /locus_tag="OHN28_004729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299985.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
BASE COUNT         1381 a          885 c          969 g         1224 t
ORIGIN      
        1 aagacagctt aaaaaaagcg aaagactcta ttacaggctt aaaggaattc gcagagaata
       61 tcaagccagc taaattaaga tttgataatg tcactaaagg ctataagaaa gcacaaagtg
      121 aagtagacaa gattgctcaa cagaaagcta aagccgaaaa gcaaaatcaa agagcacaag
      181 ctgccgcagc aagagcacag gcaaggcaac agcaacaaat agcagcacgt aaagagaaag
      241 cagaattaaa gctcctcgat gtttcaagct ctatcagtgc aatgcaccgc ctctctgtag
      301 cggaacaata caaagctatt gcacaggcta gagagatagc aaaacaatat gagcaagggg
      361 ctatctcgct tgcacgtatg aatagccaga tgaaacgcct acaacagcaa caacgtaaga
      421 ttaatggcaa cagaaaagct caactagccc ccgttaaggg aggatcaggg aattccgcca
      481 ctatgggtgc tctgttattt ggaggcgcta cagcggctgc tggcgtgatg gctgttagta
      541 aaacctctga atttatggct aacagctttg ccaatgctga aacagaagga gagctaattc
      601 aacgtgcaag gcttggcggt gtagacgtta accagatgta taatattaca gaatgggcgt
      661 ataaaaacgg cgttgactct atgatgggtg atcaaggggc taggaaatac ctggatcaga
      721 tgaaagatgt tagggaacgt gccgcaaaat cctacagtga agctgaattg gtgaaagata
      781 agaaaaccgg aaaatctgaa tggaaaggcg gtgataacgc tattaatgaa cttctaaaca
      841 tcggagttat taacaaaagc gaccttaaaa aatttgctga taatccagcc ggattaattt
      901 ctaaggctgt taatggaatg atgaaaaaag ggttttcaga ctcccaaatt ggacaacgtt
      961 tagaagacct gggcgatgat ttgatgctta cgtcaaaata ttggcaacgt tccgttaaag
     1021 atgtacaaga aagtattaac cagcaaaaag cctctggaaa atggctaaca gaaacacagc
     1081 aagactctat tgttaaattc agagaactaa acagacagct ttcacagctt tctgatgcaa
     1141 gacaagtagc attcgtagac ggctttatga aatctcttga tccaaaagta acggaagagt
     1201 ttatgaaaaa cctttctaat ctcactccgt attttacgaa gctgggagaa gctacaggaa
     1261 gtctctttga agctattatg aagattgtaa actggtttaa tcgtaatgat gataagacag
     1321 atgctattca gaaaaatatg ggagatgcac cgccattaag caatgatggg atgaaacaga
     1381 atctttctaa tctcgttcct gaccaatata aaggcgctgg cactgcaaca actacacctg
     1441 ataatagtca ttctctcttt aacacgatta agggattgct tttcgatgat aattcttctg
     1501 tgtcagatgt aaaaatgtca gtcaatgaag caccgataac caatctcaaa cagggtgctt
     1561 taaataatct tgcaatgaca actccggctt ataatttatc tcctgtgttt aatctgaatc
     1621 cgacctttga agtggtaaca gaggttcctc ttactattaa ttctgacaca tcaagattaa
     1681 gcgattatgt ggattttaca gcgagagcaa gtcgggatag cttcttgaaa tcattaacat
     1741 taaccagctt atcaggacaa tccaacggcg ggtaattccc gccataacat tacaaggaaa
     1801 aattattatt atggcgaccg ccgggatttt aaccatacgt gcagcaaata ctccagaaca
     1861 acacgttcag gctgtttaca aagcagaaca agatataaac tctacaaaaa acgaaaatag
     1921 caaagctaat acaacaaaag gcgaaaatgg atttgctatt gttaccagtg gcttggcatc
     1981 ttctggcaat gacgtttatg aaaattatat ggcactggcc tttgatagtg ttgacgatgt
     2041 gaacgtgaga cgttcggcag atgttacaag ctaccccgta gaaaatggtg ctactgtgtc
     2101 tgatcacgtt caaattaaaa acaataaatt ttctcttaaa ggaaggatca ctgaaacacc
     2161 gattaaaagc gatcctggct tgttaaagag tgcaggggtg aacgggaaca gaagatcatt
     2221 agctatcgac tacctgaatc agattatgga cagtagacag ccttttcttc ttgttacaga
     2281 aaataaaact tttgagaacg ttgttttaac aggcatcgaa tacacagaag aggcaagcga
     2341 atccctggta tttgatctta gctttgagca aataagatta gtttcttatg gcactgtaaa
     2401 cactgtagct atcaaaacac agccatctaa gaacatcggg gctaatatga aaaaacgtgt
     2461 taacactgaa aaatcaagta gtgaaggtga agacactatt actcctgcct ttaaacagga
     2521 atagccgtaa acgcctctaa aacgctctgt aacgatgtaa ttataaaaga ggctaccaat
     2581 ctatgttgaa gcctccttat tgcatctatg gcgttgtttt acgccttaaa tttgattttt
     2641 tattgattga taagcctttc tctatttcag ggtagcgtag aagctcaata tagtttttaa
     2701 gctctactgg agaagctgca ttcttgctat atgtgcggaa tgtctctgta ttgccgcgtg
     2761 tatggcccag gagaagggcg atcctgtctt ctggaatttg atttcgatca agaagctgtg
     2821 ctactccgtg tctcagagaa tgaaacacct tcctttctgt tcccttttcc cctaaagcct
     2881 ttcgtttagc tcttgtaaat ctctgcgtgt gccacgtgga acgcttgccg tctgcacgtt
     2941 ctgtaatgct ggcgtgatag aacaaaaagc cattatgagg cttttcacgc agcgacaaca
     3001 ccaacggagt gataaggcta tgcacaggca caacacgcgc cgcagctttc gtttttccct
     3061 gtgtgatttc aaaacacagc acaccttcga tctctttcac atcgtctatt gtgagacttg
     3121 ctatctcatt gatccgcata cctgtatata aaccgataag acacaaagct atcatttctt
     3181 tgttttctgc tgaattaccg gaaaatactt gcaacacctg caatagctct ttgttagaaa
     3241 atgcctcata gctctctcta ctttgtgcca catcaagcct atgccctcgc cagggggaga
     3301 gcgccctttc tggcgcatcg tggtaacgtg atgaagctaa ctcccatagc tgggccattg
     3361 ggctgatata atttgcaatt gattgttgtg aaagggtttt ttgcatgtgt tcaatccagc
     3421 ctgtaacagt ggtgcggctt acatcttgca atgcaatatc aggcttttta cggtaggaga
     3481 gaaacatctc taccgctttt cttgccttag ctaaagtggc tggcttcttc ttcgtgctgt
     3541 taattgtcag gtagatttca agaattttaa gcaatgacgg acacgaagac gctgtatctt
     3601 gcattctggt agctgttttg gcgtatttag ccttactgcg taacagttcc agcgtgttct
     3661 ctattgtgct gtttacaggg gcgacactct cccgtagaca atggtattca tctgcaatct
     3721 gatcacgctt tctacgtgca acacgaagac tacttgtgtg tagacttctg acaaacgtcc
     3781 tttttccttc aaaaaagggc tgcatataca caggcagcgt gatccttaaa tagtagttac
     3841 cgtaagaatc acagattatg tattgattag gcttgtatct cataaactcc ccggctaaag
     3901 tgtcggagcg tttgtaatgt gaaaattgat tgtgacgcat agaaaatgat gatttttcta
     3961 taagattcaa tcaattagta atggtcgtag gtgatggcat taacacgaga ttaatcgagt
     4021 gttttactcc tattatcggc accacttaaa tcaataagtt acacatcatt aataccttcc
     4081 ttattttttg attgggacag atttgggacc gatgggttca ggatcgaatc tatttaccgt
     4141 gcgtgttcgg taaggtgatt aggtgcgagg tgagcatatc gacgaaccat ttcgagagtt
     4201 tcctatccgc tcatttttaa gtgcaagcag ggaaacaccg gaccaaacca atcagctgtt
     4261 cgaaaatggc aagcggatta atatgccatg gcggctttga gccgcacgca tggggtgtgg
     4321 caagcctgat ggatgttact gtgcttgtgg tggcgtcttt gctggtctgg ttgttggtta
     4381 atcgcgaaaa tcattatgcc agaggaatac cacaggacgt tcaggttttg cataaaaaag
     4441 cccaccatta aggtgggca
//
LOCUS       DAJOTB010000256         4457 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJOTB010000256 DAJOTB010000000
VERSION     DAJOTB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.257"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            246..890
                     /locus_tag="OHN28_004730"
     CDS             246..890
                     /locus_tag="OHN28_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2299986.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSY
                     YIENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRV
                     MAVNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKD
                     GKYKGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSRQSSGE"
     gene            complement(1017..2294)
                     /locus_tag="OHN28_004731"
     CDS             complement(1017..2294)
                     /locus_tag="OHN28_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCQ2299987.1"
                     /translation="MINSLSLPGYQLVDSDEQNEDIHFRLEAPTPVACEGCCVQGEFV
                     RFGKRDVPYRDLPIHGKRVTLWVVRRRYTCRACKTTFRPQLPEMVDGFRMTLRLHEYV
                     EKEAFNHPYAYVADTTGLDEKTVRDIFNARAEFLGRWHRFETPRILGIDELYLNKRYR
                     CILTNIEERTLLDLLATRRQDVVTNYLMKMKDRQKVKIVSMDMWNPYRAAVKAVLPQA
                     RIVVDKFHVVRMANDALEKVRKGLRKELMPSQSRTLKGDRKILLKRAHEVSNRERLIM
                     ETWTGAFPQLLAAYEHKERFYGIWDATTRPQAEAALDEWIATIPKGQKEVWSDLVRAV
                     GNWREEIMTYFETDMPVTNAYTESINRLAKDKNREGRGYSFEVMRARMLYTTKHKKKA
                     PTAKVSPFYKKTIGYGLPDFAEELNYGVDLSTI"
     gene            complement(2307..3341)
                     /locus_tag="OHN28_004732"
     CDS             complement(2307..3341)
                     /locus_tag="OHN28_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2299988.1"
                     /translation="MSPQLQDALGTFALLAIELPVLFIGISLLVGVLQRHIPPSKVEA
                     LLTSSGKRGYFLAAGLGAITPFCSCSTIPMLKGLIQARAGFGPMMVFLFTSPLLNPIV
                     VGLLVATFGWTLTAIYVLAALVVAVGAGWLLHTLGFERYVRRTATQESSGCSSSASPC
                     SATSTASSCGTNSCDTTPKTASCGADRKTTVSTSSECCDSQPTVTVKKEGKYNGVWRE
                     AWSDFIDVLPYLLIAIAIGSVIYGFMPTDLLAQYAGPDNPFAIPVAAVIGVPLYIRAE
                     AVIPLAAALMAKGVGAGTVLALIIGSAGASLTELILLRSLFTLKLLAAFLAVIFAMAM
                     IAGYATYLFF"
     gene            3453..3767
                     /locus_tag="OHN28_004733"
     CDS             3453..3767
                     /locus_tag="OHN28_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2299989.1"
                     /translation="MDHEKVAASLAELGNSHRLSVFRFLVKAGHDGASVGDIQKGLGI
                     PASTLSHHLARMAKVGLIRQEKHSRTIVCIPEYGHLENLIGFLQEECCAGVRIAHEPE
                     PL"
     gene            complement(3999..4178)
                     /locus_tag="OHN28_004734"
     CDS             complement(3999..4178)
                     /locus_tag="OHN28_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2299990.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCL
                     SWYITKPKPQKAEVA"
BASE COUNT         1094 a         1127 c         1171 g         1065 t
ORIGIN      
        1 aaagataagc ttgtatttat gttgtcttta ttgtgtaaag atttgcgtgt ctttatagtg
       61 gctttacatg gtaaagctga tgctgtcttt acatcatctt tatgttgtaa atttctgtgt
      121 gacattgtca ctttatggtc ggaggtctgt tttctggttc tttttgagtg tgcctttcct
      181 gtcgatccac tttctggttc attttgggta tagtgatgtg accttatgcg aactggttct
      241 ctaatatgac ttcaaatgca ccaacaaact ttgtccgcgc ttatctgaga gcttcaacag
      301 cagaacagga tgcttcgcgt gccctggaaa ctatcgaggc ttttgccagg gaaagaggcc
      361 tgatcatttg cagctattat attgagaatg aatccggctc ccgtcttgac cgcccggaac
      421 tcttccgtct tctgagagat tgccagcaaa atgacatttt gcttatcgag gatgtggaca
      481 gactttcccg actgtcaggg gaagactgga atacgctgaa gaaaatgatc cgtcagaaag
      541 atattcgagt tatggccgtg aatgtgccga caacctggat taattctggt atgagtgagt
      601 ttgacagccg gttgtttgca gccattaacg atatgctgct ggatatgctg gccgctgttg
      661 ccagaaggga ctatgaacaa cggcgggaac gccagaaaca ggggatagag aaagcaagga
      721 aagacggcaa atataaaggg cgtaaaccta atcaagccag acatgatgca attatcaggt
      781 tgatagaaag tggcagctcc tggacacagg tacagaaggt gctagggtgc agccgcggca
      841 caatcagtag cgcaataaaa cgtaaatccc ggcaatcctc cggtgagtag ggtagggtga
      901 gctcaataaa ctggctgacg ttatttatta gggaggtgct gaggtggata tttttaaagg
      961 gtatacggat ttaatggttg atggggcacc tttaccccac caacctgata ccattatcag
     1021 atggttgata gatcgacccc gtagttgagt tcctctgcga agtccggcag tccgtaaccg
     1081 atggttttct tgtagaacgg agagaccttc gcggtcgggg ccttcttctt gtgcttcgtg
     1141 gtgtagagca ttcgtgcccg catcacctcg aaggagtaac cacgtccttc acggttcttg
     1201 tccttggcca gtcggttgat ggactccgtg taagcgttgg tgacgggcat gtccgtctcg
     1261 aagtaggtca tgatctcttc gcgccagttg cctacagccc tgaccagatc gctccagact
     1321 tccttctggc ccttcgggat ggtggctatc cactcgtcca gggcggcttc tgcctggggc
     1381 cgtgtggtag cgtcccagat gccgtagaaa cgttccttgt gctcgtaggc ggccagcagt
     1441 tgtgggaatg cgccggtcca ggtctccatg atgaggcgtt cccggtttga gacttcgtga
     1501 gcgcgtttca gcaggatttt ccggtcaccc ttgagggttc ggctctggga gggcatcagc
     1561 tccttcctga ggcccttgcg caccttctcc agggcatcgt tggccatgcg caccacatgg
     1621 aacttatcga ccacgatgcg ggcctgtggc agcaccgcct tgaccgctgc tcggtaggga
     1681 ttccacatgt ccatgctgac gatcttgacc ttctgccggt ctttcatctt catcaggtag
     1741 ttggtcacca cgtcctgacg gcgggtggcc agcaggtcga gcagggtcct ctcctcgatg
     1801 ttggtcagga tgcagcggta gcgcttgttc aggtacaact cgtcaatgcc gagaatgcgg
     1861 ggcgtctcga agcggtgcca gcgccccagg aattcggcgc gggcgttgaa aatgtcgcgt
     1921 accgtcttct cgtccaggcc cgtggtatca gccacgtagg cgtaggggtg attgaaggct
     1981 tccttctcca cgtactcatg caaccgcagc gtcatgcgga agccgtccac catctccggt
     2041 agctggggcc tgaaggtggt cttgcaggcc cggcaggtgt accggcgacg aactacccag
     2101 agggtgaccc gcttgccgtg gatgggcagg tcacgatagg gaacatcacg cttgccgaac
     2161 cgcacgaact caccctgcac gcagcacccc tcgcaggcta ctggtgtagg tgcttcaagc
     2221 cgaaaatgta tgtcttcatt ctgctcatca gaatccacca actggtaacc aggaaggctt
     2281 aacgaattaa tcatctcccg ccttgatcag aaaaacaggt aggtggcgta accggcaatc
     2341 atcgccatag caaaaatgac tgctaaaaac gccgctaaaa gcttcagcgt gaacaaagag
     2401 cgcaacagaa tgagctcagt caggctggct ccggcactgc cgatgatcaa cgcaagcacc
     2461 gtcccggcac ccacgccttt ggccatcaga gccgctgcca gaggtatgac ggcttcagcg
     2521 cgaatataca acggcacgcc gatgacagcg gcaactggaa tggcaaaggg attatctggg
     2581 cctgcatact gcgccaataa gtcagtgggc atgaaaccat agatcacgct gccaatcgca
     2641 atagcgatga gcaggtaagg caacacatcg ataaagtccg accaagcttc ccgccacaca
     2701 ccattgtact tcccttcttt cttaaccgtg acagtgggtt gactgtcaca gcattcgcta
     2761 gacgtagaga ctgttgtctt cctatcagcc ccgcaagaag ccgtcttcgg agtggtgtcg
     2821 cagctgttgg tgccgcaact cgatgcggtc gatgtagcac tgcacgggct tgctgatgaa
     2881 ctacatccac tgctctcttg tgtcgccgtc cggcgcacat aacgctcgaa gccaagcgtg
     2941 tgaagcagcc atccggcacc taccgcgacc accaaagcgg cgagcacata gatagcagta
     3001 agtgtccatc caaacgtggc aaccagcagg ccgacgacga taggattcag caacggagat
     3061 gtgaaaagaa acaccatcat cggcccaaaa ccggcccgtg cctgaatcaa ccctttcaac
     3121 atggggatag tcgagcaact acagaagggc gttatagctc ctaaaccagc agcaagaaaa
     3181 tagcctcgct tcccactgga agtcagtaac gcttccacct tggatggtgg gatgtgacgt
     3241 tgaagaactc cgaccagcaa gctaatgcca atgaataaaa ccggtagctc gatagcgagc
     3301 aaggcgaacg tgcctagagc gtcttggagt tgaggtgaca ttcaatatta ctcctatatt
     3361 tctagtttta tcgaaatgat gtacgcagtc tacttgcgtt tatccgacct gtcaactata
     3421 attctagaaa catcgaatta ttggggcgtg ctatggatca cgaaaaggtt gcagccagct
     3481 tagctgagct agggaatagt caccgactgt ccgtgttccg ctttctggtc aaagctggcc
     3541 atgatggggc ttcggtcgga gatatccaga aggggctggg tattcctgct tcgacgctat
     3601 cacatcacct tgcgcgcatg gccaaggtag ggctgatccg gcaggagaaa catagccgca
     3661 cgattgtctg tatacccgag tatgggcacc tagagaattt gatcggtttc ttacaagagg
     3721 aatgttgtgc aggtgtccgg attgcgcatg aaccagaacc gctttgacgc ttgcccagct
     3781 ctcgctgtcc tccctagtca gcatcgctat gaagactcag gcgaaggtgg aaacagaaaa
     3841 ttggcagtag agtaggctga agaggatcaa tcagataatc cgctgagaaa gattttcttc
     3901 tttacatctt tggcatttta tcaaccagaa attccggata ccctttttaa aaagcaaatt
     3961 gaatgttgta actaatgaat atcaaaataa aaggccgctt acgcgacctc agccttttgc
     4021 ggttttggct tggtgatata ccacgataga caaccgacaa taaaactcat gaacattcca
     4081 gtaaaacgat catcgatgcc tggaaacagg caaccgacaa acatcgcaac agggaaagga
     4141 agaagtaaac agcgccccaa aatgctcata aaatccataa atcctccttt gttaaagggc
     4201 aggaagcggt gcacttcctg cccttagtta cgtttccgca gtcatcaatc gatgaactga
     4261 cggatcagtt ccagaactgc aatcagcgcg tttgcaacgg cgaccacagc cctgagaacg
     4321 atgattaaca ggttaatcat ccgtttctcc aggttgatac tggaggaagt cgccgttgct
     4381 tgctcataca tcgcctgtca caacggctct ctcacattgt tggatgtaga gtaccttccc
     4441 cctgccagag cactgaa
//
LOCUS       DAJOTB010000257         4427 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJOTB010000257 DAJOTB010000000
VERSION     DAJOTB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.258"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            85..1308
                     /locus_tag="OHN28_004735"
     CDS             85..1308
                     /locus_tag="OHN28_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2299991.1"
                     /translation="MGTIQKIRTDVNVLYAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1293..1937
                     /locus_tag="OHN28_004736"
     CDS             1293..1937
                     /locus_tag="OHN28_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCQ2299992.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
     gene            complement(2308..4425)
                     /locus_tag="OHN28_004737"
     CDS             complement(2308..4425)
                     /locus_tag="OHN28_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCQ2299993.1"
                     /translation="MLSTLSLCISGIVSTATATSSETKISNEETLVVTTNRSASNLWE
                     SPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLILI
                     DGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGDK
                     LASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYRN
                     NSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFYD
                     TDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQA
                     VTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQS
                     TVSLFAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDHE
                     MVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNNF
                     ELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRAK
                     TWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQA
                     NIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDKR
                     YRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT         1141 a          958 c         1103 g         1225 t
ORIGIN      
        1 ttaaggtaga actttttctt gatgagaagg tttaagtttg tagttcagaa tattactgcc
       61 aagatagttt atactaggat atgtatggga accatacaga agataagaac agatgttaat
      121 gtattatatg cggccattca ccgtattgag tggttgtttg agacattttc atctgtttgt
      181 ttgtcttttt ctggaggaaa agactctact gtgctgctcc atcttacggc cgatgtggct
      241 cgcaggaaga aacgtcgttt ctctgtatta ttcattgact gggaagctca gtaccagtgc
      301 acaattgcac acattctgaa gatgagggag atgtatcggg atgtgacgga gactttttac
      361 tgggtggcac tccccctgac tacggtaaac ggtgtctctc agtttcagcc ggaatggata
      421 tgctgggaac caggtgtgga gtgggttcgt cagccaccag atgacgctat tacagatatg
      481 tcgtatttcc cattttatcg gtatgccatg acgtttgaag aatttgttcc ggcattttct
      541 tcctggtttg ccggtaaccg gtgtggagtg gcaatactga ctggtgttcg tgctgatgaa
      601 tcgctcaatc gctttatggg actggtgtct cagcgcaaac tgagatatgc agatgataaa
      661 ccctggacca cagcgtcacc tgaagggttt tattacacct tgtatccgtt gtatgactgg
      721 aaagcccgtg atatatggat atataacgcc agaacccgag ctatctacaa tcccctgtat
      781 gacctgatgt accgtgccgg cgtgccgtta cgcaacatgc gggtctgtga gccttttggc
      841 ccggaacagc gtaagggact gtggctttac catgttctgg agccggaaac ttgggccagg
      901 atgtgtgagc gggtgtcggg tgctgccagc ggggcgcttt atgccaatga gagcggtgcc
      961 tattttgccc tgcgtaagcg tatcacgaag ccacctcatc atacctggcg tagctatgcg
     1021 atgttcctgc tggatgtgat gccggaaaga acggcagaac attaccgtaa taaaattgct
     1081 gtctacctgc gctggtatca gacgcggggc ttcccggatg acatcccgga tgaacaggag
     1141 aatgacctgg ggagccggga tatcccgtct tggcgacgta tctgtaagac actcataaag
     1201 aatgatttct ggtgccggac cctctccttc agtccgaaca aaccccggca ctatgaacgt
     1261 tatctgcagc gtatgaaaga aaggaggaag gaatggggga ttctgtgaca cctgaggtgg
     1321 aagtattgag caacatgatt cgacagtact tcagtcagga acgatctgaa gaagagacca
     1381 tacgagccct taatcatctg cgcagggtgt tgcatgaggt cagccctttt gcacaggagc
     1441 ctgtggactg tgtgttgtgg gtgaaagcgg atgaggtggt ggccaacgat tacaatccca
     1501 atgtcatgtc ttccagtgaa aaaaaactgc ttaaacattc actggagcag gatggattca
     1561 cgcagcctgt ggtggtttcg gaagagaagg agcattatct ggtcgtggat ggttttcatc
     1621 gtcagttgct gggcaggaaa gcggatacga gaaaacgtct gaagggttgg ctgccagtga
     1681 cctgtattaa tccggaaaga aagggacagg catcacgtat tgccgcaacc atacggcata
     1741 accgtgccag gggaaaacac cagataacgt caatgtctga cattgtcagg gacctttcgc
     1801 gtctgggctg gacagatgag cgtattggca cagagttggg catggaccag gatgaagtgc
     1861 tgaggctgaa acagattagc gggctgacag agctgtttca ggaagagaat ttcagccctt
     1921 catggacggt tcgttgatat tgtaaaatat gatagttgga gtaaggggca atagaaggag
     1981 aatatttatc ccaccttctt ccgagtcggg ataaatcaaa aggtattatc cattgattca
     2041 gtatctgggt gagagtaaat aatctaaaaa tagtattgcg ttgtgaactg atatcacttt
     2101 tgaattacaa aggtataggc tcagtaacca gctttataag tcattgtcgt agcaatgact
     2161 atagagtcat gctggcggga agaataaata ttcaccccca gatggaaaac aacgtcgaaa
     2221 aaccttcaac aaacacaata cgttcgttag cctgcccgca aagcgggcag atcaggacaa
     2281 agaattccat aacgattgtt tttgtcatca gaaattccat acaaaaccta tagctgcatt
     2341 aaaacctgct gcaggaatag tttcatgtgc tgtacggtag cgtttgtctg tcaggttatt
     2401 gagtgctagg ttaatccggt actgatcctc gttaccgaat tctgtattta ctgcaaagtt
     2461 gagagtggcc cagcccggaa catttgtttc ggtaccgtta ctgtcatctt ttgcactaga
     2521 ggcagcacga ataaaaacat cagagattat gttggcctga cccatcacaa gagtatgttt
     2581 cagccctata cgtccgttta tcgctggttc tcctgtatta gttgttttta atgttgaagt
     2641 ttcatattgc cgacgaatta aattgccact gatatatggc gagaaaaccc agccattata
     2701 ttccgcgctt atttccagtc cccatgtttt tgcccgatca atattgtcat aataatagta
     2761 gctactacgg gaggagttgg tgttaccatt gcaaactata ctgccatcac agatcagact
     2821 tgcaatataa tctttagctt ctgagtagta aactgcgctg tcaatcagcc actgattacc
     2881 attatatcgt gcacctaatt caaagttatt ggagtgttca gccttaagat caggatttcc
     2941 gtatgtgaca ctgccgcccg cagatgtctg cataaaaagc tgggagagtg tgggaaatac
     3001 gtagccttgc gcgaacgcag cgcgtaactc caagttatcg aaacctgaat agcgcaggct
     3061 tgtagatgtt accatttcgt gatcactcgc agactctctg gcaagagagg tatcgcttat
     3121 aatgcctgcg gtatatgata ctccgtcacc acgcgtcaac tttgaagaaa gccagtattg
     3181 gcgaactccc attgtccatg tccagtgatc ggcgaatcgc cagtcatttt gtgcgaatag
     3241 tgagactgta ctttgctctg actcataata ggagcgagtt cgtgtttcct tattaatgaa
     3301 gccggtcaga gatttgcttg aggtcatgcc accggacctt tggctgaccc tgtcttgttt
     3361 gtactgtgca ccggtaacaa gttcattatt agcaggcagc gaaaagtgac tctgcaatgt
     3421 gaccgcctga gtgtattgct tatcatgggt gtcagtcttg ttatgaacgg tcagagcctg
     3481 aatcatcgga ctgggaacag gctgtgtcgt ttttacttcg ttggcaaatt ggcgctggat
     3541 ggtctgctca tacgcgtcga aatgaatttt ttttagatag tcaccgtcca cgtctgtgtc
     3601 atagaatacc ccaactttct ctcgttcaag tttaggtatt ttgacactaa atgcctcata
     3661 gcttccgtct ggatcctcat agtaagtttg cgtcgcgagt ctgtagcgat caagcgagag
     3721 gccaaaacga tggtttccgg agttataacc caaccataca ccctgactat tgttacgata
     3781 gttggtattc ggcagacgtc catccggcgt atcacgattg ccctgatcag aataactacc
     3841 gttgatgcga taatcaaatc caccgatgct cccctggacc gcgattgatt cttcccagcc
     3901 tgctgttgcg gaattataaa cagctttcac aactccagat gcaagtttgt cacctccctt
     3961 tttggttatg aagttaacaa taccgccaat tgcctgtgaa ccgtacagta cggaatatgg
     4021 acctttcact acctcaacac gctccagcgc agactcatct atcaacagtc ccacaccata
     4081 attatctccg gcgcgctgat aagttacctc ctgaccatca atgagaatta aaacacggga
     4141 ggatgcttcg ccacgaatgc ggatttgttt acggcctgcc aaggagttgt ctgttatctc
     4201 tactccgggg atgtcctgca aattatcggc tatggaggca ttggtggagt tctgcaatgt
     4261 ttgttggtca ataacctgta tagtcgccgg gctttcccaa aggttgcttg ccgaacgatt
     4321 cgtggtcacg acgagcgtct cttcgttgct gatttttgtt tctgaagaag ttgcggttgc
     4381 agtagaaact atcccagaaa tgcataatga cagagtggaa aggatat
//
LOCUS       DAJOTB010000258         4412 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJOTB010000258 DAJOTB010000000
VERSION     DAJOTB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.259"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..250)
                     /locus_tag="OHN28_004738"
     CDS             complement(<1..250)
                     /locus_tag="OHN28_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2299994.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPA"
     gene            complement(247..726)
                     /locus_tag="OHN28_004739"
     CDS             complement(247..726)
                     /locus_tag="OHN28_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2299995.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINPSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(723..1214)
                     /locus_tag="OHN28_004740"
     CDS             complement(723..1214)
                     /locus_tag="OHN28_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2299996.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGITTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(1231..1983)
                     /locus_tag="OHN28_004741"
     CDS             complement(1231..1983)
                     /locus_tag="OHN28_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2299997.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(2003..>4412)
                     /locus_tag="OHN28_004742"
     CDS             complement(2003..>4412)
                     /locus_tag="OHN28_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCQ2299998.1"
                     /translation="INWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWTHNDECL
                     KPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWDPPSRWDDGISGLIADYSLNAQT
                     RHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDSSNSTTSKNWD
                     WSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDVS
                     GVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVT
                     TASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASWGVADGWSLYGGALADEH
                     YQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNS
                     RVTFAGYRFSEKNFMTMSEYLDASQSDMARTGNDKEMYTITYNQNFAAAGVSVYLNYS
                     HRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGYRYEYDDNTDKGMYLSMSIPWSD
                     SSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQINVGTSEQHGSVDGYLSHDGTLAKVD
                     LSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNMGGTRLLIDADGIANVPVESNGA
                     PVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVI
                     SGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDDEGNVYLAGINAGEHMMVFWEGS
                     AQCEIVLPKPLPADLFRGLLLPCEQKGTTAPDSLAPEIKPVIQDQTRQVTPSEAPTSI
                     SATQ"
BASE COUNT          907 a         1247 c         1145 g         1113 t
ORIGIN      
        1 gagcaggccc tttgcaatct ttaagctgaa acaccaccgg tacgcgcgca gattgcccgg
       61 tcaccatcag atctctacgg ctgaccgtgg ggaaatgcac ttccgccagc acctcgccct
      121 gaaccaccag cgtgcaggac ttactgagta aattgccgta gaaatgcagg ttgtctgccg
      181 cctgaacatt cggcgtcagc agcaccgcgg ccagcgcagc cgcgcagtga aatatccgtt
      241 ttaggctcat tggtaatcca cctgtaatgt tgcccaggca gaaaaggttc cttcgctgag
      301 tgaagcatca ctggctttca ccggtacagc ttgcagcgtt ggcggcgttg acgggttgat
      361 tttcagcggc gtacccaacg tgaacggctg gccattttgt tgcagctcaa tccccagccc
      421 ggtgacgttt gtctcaatag ccgcgttatc gtagctggtc tggctgcccg tccagctcag
      481 cgtcatctcc caggcatcgt cgcgcacgtc gggatcgcat gtaatggtat agggcacatt
      541 ctggcggaag ttattgccgt tgatgttgtc gataatgacg tcacgaaact caacttcaac
      601 ggttttcccg tcactgatgg cacaggcggg cggcgaaacc aatgtgccat gaaatgtgat
      661 atcgctatcg gccgcctgtg caataccgca ataacaaccg aataagcaga gcgcaaatcg
      721 tttcattgat actccaccac cattgtcatg gttgcgctaa actctgatgc cgtaagctgt
      781 gtgccgcttt gtttcaccgg cacggcttcg aaacccgggc gcgaattaac gttaaacggc
      841 agccagttat tacccgccaa cgcaaccggc gcatggctgg acgcatcctg tatgcgaatg
      901 ccaaaaccat tgatgcccgt actgaggacc gtttcaccgt taatggtggt ggtgatcccc
      961 tgtaactgca tctgtaggtc atcgctgacg ctgttcgaac agttcagggt gtaaccaaca
     1021 ggctgccgat agttgctgcc gtcgatgttt ttaataatga cattgccaaa ctccaccgcg
     1081 ctgcctttta ccgtacaggg cggcggtgca tccaccagta tacgaagcgt taagttgacc
     1141 gttttactat cggcccaggc ggagccgcag gccagtaaca ccatgccagc gacgggtaat
     1201 aaaactcctt tcatgctgga tctcctgtca ttagcctttt aggctttgag cttcatccac
     1261 cttgcaggtg tctccttcac aacggaagac cagcggtaaa cgtgcgccgt agtcgttgat
     1321 gtaggtcagc accggagcgc tgttcatttt tgcctttaac ggttgtgacg ttttcggcgg
     1381 catcaccagt ggtttaaagc ccgcagcagt tagccccttt ttctgcgtac tggcatcgct
     1441 gatgatgacg tggtacggcg tcgggttatt gacgatatag tcctggccgc tgcgcgtgag
     1501 tgtgacctta aactgccacg gttttctcat gtcaatcttc tccagcgcct ttggtcgcca
     1561 gaacagcttg atgcgggttt gcagcgcaat ttgcagggtg ttggctttcc cggactttgg
     1621 cgggatctca cgcacattgt agtagaacag gctttcacgg tcagacggca gcgtgtgaat
     1681 gtccggcagc gcctgaatct tcacctgacc gttcatcatg gcgtcaatac gctgcaccgg
     1741 cggcagtacc gccagcggcg aagtgatttt gttgcccttc tcgtcttcca gccagctttg
     1801 cgccagataa ggcagctttt cgttattgtt gcgcagggtt acgcttatcg atttatcact
     1861 ttcgttaaac accagacggg tgcgatccgg agtgactgat gcgtgcgccg ccaggttggt
     1921 ggtggtcaac agtaccgcca gcgtcgtgct gaccaccatt gaacaggata atttataaaa
     1981 cattgattgt tccttaatca tattattgag tggctgaaat tgacgtcggc gcttcagatg
     2041 gcgtgacctg ccgagtctgg tcctgaataa cgggcttaat ttccggcgct aaagaatcgg
     2101 gggctgtcgt tcccttttgt tcacacggca acaacaggcc gcggaacaga tcggcaggta
     2161 gcggcttcgg caatacgatc tcgcattgtg cgctgccttc ccagaacacc atcatatgct
     2221 cacccgcatt gatccccgcc agatagacat tgccttcatc atccacaatg ccaacctgct
     2281 gctgctcgtc gttttttact tccgcgccaa acggcgggta gctaccgtca cgcaaccgca
     2341 gtaccgccat cgctttttgc ccgctgatca ctttgaattt acgatagccg attgcgcctt
     2401 ccgtcagagt cgcctgcacc accgactggg tggcttccgc atcttccggc aagttgttta
     2461 aatctatata cgcctgattg cgatagtagt tgttaatatc ggcgaccacc gccttgccaa
     2521 acatattggt gtacaccggc gcgccgttgc tttcgaccgg cacgttggca ataccgtcgg
     2581 cgtcaatcag caggcgtgtg ccgcccatgt tttgagtgcg atgcagcgcc ccaccgtgcg
     2641 cggtcagcgt tgccccgccc tgtaaggcga tccccgccga acggtattct ccttcatggt
     2701 agttggcgct gagatctacc ttcgccaacg tgccgtcgtg gctcagataa ccatccacgc
     2761 taccgtgctg ttcgctggtg ccgacgttaa tctggtagtg cgttgcgtca tcgatgcgat
     2821 taaagtagcc gacctgactg ctgtccgacc cgctaccgta agagccgttg taggtcacgg
     2881 tgctgctgtc gctccacgga atgctcatgc tgaggtacat tcccttatcc gtattatcgt
     2941 catattcgta gcgatagccg gtcaccgaga tgctcatatt gcgaatgctc cccatattaa
     3001 aatagtggga aaacatcagg ttatagtttg tctgttccgg gcgatcccag taagtacgat
     3061 gggagtagtt gagatagacc gagacacccg ctgcggcaaa gttctggtta tacgtgatcg
     3121 tatacatctc tttgtcgttt ccggtccgcg ccatgtccga ctggctcgcg tccagatact
     3181 cgctcatagt cataaagttc ttttcggaga aacggtagcc tgcaaaggtg acgcggctgt
     3241 taagttcgtc aaagtctttg gcatagctca cgcgaaagga gttgccgtcc agttttcctt
     3301 tgccgtatgc gctgtcatga tccaggttga cgtgcgagtg agtcacatcg aacgccagcg
     3361 cgccaaactg tgcgaggtcg cgccccaccc ccatcgccgc cgactggtag tgctcatctg
     3421 ccagcgcgcc accgtaaagc gaccagccat ctgccacccc ccatgatgct tctccgccgg
     3481 aaaaaaatcc gccttcggtc ttgtggttcc agtcttccgg acgccccatc atcactttgt
     3541 aacgcacctg cccctgacgc gtgaggaatg gcatggatgc ggtggtgacg tcatattcct
     3601 gcacctgacc attctgttct tcaacacgga cgtgtagtgt gccagagaca gaatcgccga
     3661 tatcctgaat gcggaatggc ccggcgggaa cctgggtttc gtaaagtacc cggcccatct
     3721 ggctgatggt cacttttgcg ctgctgtgcg ccacgccaga aacatctggc gcatagccgc
     3781 gcagattggg cggcagcatc tggtcatcgg tgctgacact gctgccgata tagttaaagc
     3841 cgtcgaaaat atcggaattg agataatctt ctcccagcga cagcttcgct tttaaagagg
     3901 gtaaagctcg ccaggcgtaa taacggctcc agtcccagtt tttactcgtt gtactgttac
     3961 tgctgtcatc ttcatcgtcg ttgctgcggg tgtgctgata atcactttgc cagtctgcac
     4021 ggaaacgcca tgccccaagg tttgccccaa ctgtaccgtt accgctgata tcatgtgagt
     4081 cctcgccccc ttgctcctga tggcgagttt gcgcattgag gctgtagtcg gcaatcagcc
     4141 cagaaatacc atcgtcccag cgtgagggag ggtcccagtc gctgctggta tattcgaggt
     4201 aagcctgtgg cactgtcagc agcaaagccg actggcttaa atcattctcg acttccatcc
     4261 cgtctaattg ccccggttta aggcattcat cgttgtgcgt ccactgcagg ctttttgcta
     4321 tcccttcttt cagccccagc gccgccacta actcaggtgt caggcaggca taagtttttg
     4381 ttggatcgtt ttcagagaca taccaattaa tg
//
LOCUS       DAJOTB010000259         4388 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJOTB010000259 DAJOTB010000000
VERSION     DAJOTB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.260"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(273..686)
                     /locus_tag="OHN28_004743"
                     /pseudo
     CDS             complement(273..686)
                     /locus_tag="OHN28_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            684..941
                     /locus_tag="OHN28_004744"
                     /pseudo
     CDS             684..941
                     /locus_tag="OHN28_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1175..2032)
                     /gene="sitD"
                     /locus_tag="OHN28_004745"
     CDS             complement(1175..2032)
                     /gene="sitD"
                     /locus_tag="OHN28_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCQ2299999.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(2029..2886)
                     /gene="sitC"
                     /locus_tag="OHN28_004746"
     CDS             complement(2029..2886)
                     /gene="sitC"
                     /locus_tag="OHN28_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCQ2300000.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2883..3710)
                     /gene="sitB"
                     /locus_tag="OHN28_004747"
     CDS             complement(2883..3710)
                     /gene="sitB"
                     /locus_tag="OHN28_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCQ2300001.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3710..>4388)
                     /gene="sitA"
                     /locus_tag="OHN28_004748"
     CDS             complement(3710..>4388)
                     /gene="sitA"
                     /locus_tag="OHN28_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCQ2300002.1"
                     /translation="QLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYE
                     GKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQRNADTYKAKITQTLAPLRKQI
                     AELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIP
                     AVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGI
                     KAGKREK"
BASE COUNT         1199 a         1139 c         1020 g         1030 t
ORIGIN      
        1 aatttatatc ctttgactac atgcaagata agggaaggtt ctatttattg acattaatac
       61 cctcgacaat tgctgagcag aagcccccac gacaagtgac agaatgacaa catctacagt
      121 ggggagcgtt ttatcgatga acaagcaacc aagaagtaac acggcatagg tgacgacaat
      181 gccagtaagc atagaaataa caaaaacgtg agctttcatc attcctccag aataaccccg
      241 acgtgtaagc ctgggggatt ggcacaataa tgtcatttaa aggcagactt tccggcaaat
      301 tgcgccgatg aacccagttc atcctcaatt ctcataagct ggttatattt ttcaatacgc
      361 tcaccccggc atggagctcc cgttttgaga tgacctgcgc gcattgcaac tgtcatatcg
      421 gcgataaagc tgtccactgt ctcaccactg cggtgtgaga taaaggtgcc ccagttgttg
      481 tcatggcaaa gttgtaccgc atcaaaggtt tcactcaggg aaccaatctg gttgagttta
      541 atcaatgctg aattggcaag gttttcgtca atgccgcgct gaatatactt tacgttggtc
      601 acaaacagat cgtcgccaac caattctatc tgctctccca atgcaccatg caggattttc
      661 catccagccc agtcatcttc agctaccgtt aagggcagga agatcaacta ggtgctctgg
      721 ggctggtgac caatgcggtt gtgctgtgaa acacacttta catgcaggag gcgttgtcac
      781 atcttcgcaa atcgggagaa actcctgagg agtagcatcc ggcaagatta tcgctgctga
      841 tacatagcca tataaatatg ctggggcatt atacgtcttc gttaccggaa gatattctta
      901 agggtgagtt aagatcatta aacttcaata caaacaatta attaaactct tagcgtggtt
      961 ttttacatca ctggccctcg ccccccaatt ttctctatgt tttgccacta cttccctcat
     1021 tttactgcgc tgtttgctac ccctgagaat cgacattact tgctatccag tccgcagcaa
     1081 ctctctggca acaaaactta tcagaatcat aagtctgtgc attattcgtt caagcgcatc
     1141 gccaataagc ggatgttgct ggccgagtgt tctgctaatc ggatgataac aaatcccgtg
     1201 gttcggcatt ttcctttgtg cgtgcgttga tgctggtaac ggtaaacgtc atgatgaaca
     1261 tgaccgcgaa taagacaaca attgtcggag ctggcgcgct gtcgataaaa aaggacagcc
     1321 agacaccagt cattgagacc aatatcgata tgccagtcgc cagcaggagc gcaatatgaa
     1381 agcgttgtgt caggagtacc gcaatggcac caggcgcaat gagaagagaa atagagagaa
     1441 tgatacctac cgctttcagc gtcgccacaa tggtcaggga gaccatgcac agcagcccat
     1501 aatgcagcca tctcgtatgc aaaccgcttg cctgcgcctg ttgataatca aagctgaaca
     1561 gcaaaaaatc ccgccatttt acgctaataa caagtgtaac cagtccggca ataatcaccg
     1621 tctggattat atcgccgatg gttatcccga gcatgtcgcc gaaaagaata tgatcaagat
     1681 gtacgtctgg tttgactgca atatacaaga tcagacctgc ggcaaacatg ccagaaaaaa
     1741 caatccccat cactgtatct tgcttgattc ggctgttgtc tttcaggtat cccgtcgcca
     1801 ccgcacagaa tactccggcg acgaatgccc ctgtagccag aggtaacccc agaatccagg
     1861 caagcacgat gccaggaaaa accgcatgac tcatcgcgtc acccatcagt gcccaacttt
     1921 tcaggaccag aaagaccgac agcagcgcac agggtatgga aacaaccagt gaaatcagca
     1981 gtgcgtgact cataaaggtg aactgcagtg gctccagtaa taatgccatc atgagcgatc
     2041 ctccagcgct ttatgcgcac gccgacgatt tgccaaaagc ccatgggtgg gcgcaaagac
     2101 aaatgctaaa agaaacagca gtgtctgcgc caccacgatg attccacctg tcgcgccgtc
     2161 cagaaagtaa ctgacccacg cgccgaggaa actggtcaca ctgccaatag ttacagcaat
     2221 catgagcagg cggggaaaac gatcggttaa caaccatgct gttgcaccgg gagtcaccac
     2281 cagacagatc accagaaagg caccaaccgt ttgcagcgcc gcaacggtcg aaaccgacag
     2341 cagggtgaag aagaggattt ttaatctgcc cggatgcaat ccaatagcac gggcgtgatt
     2401 ctcgtcgaaa aaggtcacca taagatcttt ccatttgaaa aaaagcacga tgatcgagag
     2461 aatgccaatg atagtgagtt gcaggatatc cgccggatcg attgccagaa tattgcccag
     2521 gacgatggtc tgaatgttca cggatgtcgg gttcaatgac accataaaca gccccaggcc
     2581 aaaaaaggac gagaagatca ggccgatgat ggcatcttct tttaagcgag tgagttggtt
     2641 aagaaacagc atgctgcccg ctgccagtcc gccggaaaag aaggctccca gagaaaacgg
     2701 aagccccagc atatatgccc ccgcaacacc cgggacaata gagtgcgaca gcgcatcgcc
     2761 aatcagcgac cagcctttta gcatcagata gcatgacagg aaagcgcaga gaccgccgac
     2821 catcgccgag acccacatcg cattgagcat atattcatag ctgaagggtt ccagtagcac
     2881 gttcatcttt cttccctctg caccgctgac ggtcgatgtg ccacaaaagg gcgttcatcg
     2941 tcagtaataa tgctttcttc cgagccgtta agtgtgacat ggcgcagtac gccgctaaaa
     3001 gccagctcca ggttctcggc tgtaaaagtg gtgtctgtcg gcccgcttgc caggacggtg
     3061 cctttgacca tgaccgtata atcgcaaaat gtcgtgacgg atccgagatt gtgcgttgag
     3121 acaagcatcg ttttgccttc cgcgcgtaac tccctcaaca ggctgatgat tttcgcttcg
     3181 gttttcacat caacgccggt aaaaggttca tccagcagga tcacatcacc ctgctgtgca
     3241 atcgctctcg ccagaaagac gcgtttcttt tgtccaccgg aaagctcgcc gatttgtcga
     3301 tggcgaaaat ccaccatatc gacacgttcc agcgcatcgg tgacaatctg acgatccctc
     3361 tttttggcga tacgcaacat acccatatgc ccatagcggc ccatcatcac cacatcttcc
     3421 accagtacag gaaatgacca gtcaacctct tctgactgag ggacgtaggc aaccaggttt
     3481 ttctgtagcg cctgtcgtgt gggaataccc agaacagata tcttcccgct ggtcagacgc
     3541 acaaatccca taatcgcttt aaacaacgtc gattttccgg aaccgtttac cccaaccaga
     3601 gcggcaatag atccgcctgg tactgtgaag gatgcatcgc gcagtgctgt gtgcccgtta
     3661 cgccaggtga cagtaacatc attaacgaca atgcctgcag attgcatcat tatttctccc
     3721 tttttccggc tttaattccc tgtaccagcg tactggtagt aaccttcaga aggtcgatat
     3781 acgtaggtac cgggccgttt tctgtgctca gggaatcgac atagagcaca ccaccgtagt
     3841 gcgcgccggt ttcacgcgca acctgacgcg ctggtttatc ggaaatcgta ctctcgctaa
     3901 agactgccgg gatatgattt ttcttaacta tatcaacaac cttacgtacc tgctgcggtg
     3961 ttccttgttg atcggcatta atcggccaca gataaagctc ttttagcccc aaatcgcgtg
     4021 cgagataaga aaaagcccct tcactggtga ccatccatcg ctgattctca gggagttccg
     4081 caatctgctt acgcaggggg gcaagggttt gggtaatctt ggctttataa gtatcggcat
     4141 tacgttggta ggtttgtgca tttgccggat cgtattttat aaacgcatca cgaatattat
     4201 cgacgtaaat cagagcatta tctggcgaca tccaggcatg ggggttaggt ttgccctcat
     4261 agggcccttc ggtgatccct actggcgtca cacccgaaga gacaattact tctggaaccc
     4321 cattgagatg ctggtaaaag cgttggaacc acaattccag attcatacca ttggcgagaa
     4381 tcagttgt
//
LOCUS       DAJOTB010000260         4386 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJOTB010000260 DAJOTB010000000
VERSION     DAJOTB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.261"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(164..677)
                     /locus_tag="OHN28_004749"
                     /pseudo
     CDS             complement(164..677)
                     /locus_tag="OHN28_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(738..842)
                     /locus_tag="OHN28_004750"
                     /pseudo
     CDS             complement(738..842)
                     /locus_tag="OHN28_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(844..1167)
                     /locus_tag="OHN28_004751"
                     /pseudo
     CDS             complement(844..1167)
                     /locus_tag="OHN28_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1913..2890
                     /locus_tag="OHN28_004752"
     CDS             1913..2890
                     /locus_tag="OHN28_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2300003.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKMGITLDDLEKLFKSR"
     gene            complement(3175..3915)
                     /locus_tag="OHN28_004753"
     CDS             complement(3175..3915)
                     /locus_tag="OHN28_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2300004.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4036..4224)
                     /locus_tag="OHN28_004754"
     CDS             complement(4036..4224)
                     /locus_tag="OHN28_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCQ2300005.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT         1083 a         1108 c         1061 g         1134 t
ORIGIN      
        1 gctttttcgg ttgaaaagaa attgattgaa ttactggaaa aacaatctgg tctccatacg
       61 tctgtggatt gcagtactcc ccttacagag cagagtctgt cgagaaatga cggtgaagac
      121 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
      181 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
      241 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
      301 catcgagtgt tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc
      361 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
      421 ggtacccttc acgccagaca tggtttaagg ctctgcatgc ggcggcaata atgtcccggc
      481 tgtcctgtgt gggcaatgaa agcttttcca cagcggcatt gctgtaacag ggttcagggg
      541 gatgtccgta caaatgtcgt cacctgccgg caatactgac gctccccacg tagtttctct
      601 gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt
      661 tcaccaaaac tgcgactatg tcaacgccac gatgtttgac cgttatttgc cattttcatg
      721 cccgctaaat actttcgtta ctgatgctgt ccgctttgca ccatttcatc atcactgatt
      781 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
      841 ccttgttctt tttgacggac gcattttacg tttgggctgg atgtagtaac gcagcatgga
      901 acgtccgccc gtataaccca ttgccttaat ctccgcgaag ataacctcgc tattccagac
      961 attctctgcc aggcgcatgt cgatgtaatc cataaacggt ttcagcttaa ccattttgtg
     1021 gcgggtcttt ctggctggcg gttcagggta tttgaggtag cgtctgaccg tccgttcaga
     1081 gcaaccaatc tgagccgcaa tatcgacaat gtacgcgccc tgctggcgca tttgctttat
     1141 catgtaaaag tcctctctgc tcagcatgtt gatgtccttt ctggtgtgag aaccttaagg
     1201 aaacaacatg ttgggtggag cggacaatcc aaatggtgaa ttaccgtctt atatcactgg
     1261 cgctgacacc tgtagtttgt atacacaatc cctggcattg aaacacacat atgaagtgta
     1321 cttcggatat gaccgtacgc tcaggaagta cggcagattc cgtcaaaaat tacctgtaaa
     1381 gacagacatc agaaaaaaca gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac
     1441 cggcgaggaa gcgcaatgta tctgtagtta cagggagcag gtacgccctg aagccctttc
     1501 ataaaacggt tgtgtgcgct gtccttctgg aatgatttat ccacatatcc ggaggcccga
     1561 tccagtaaac agatccatga atgatcaaca aaggatccat taaagatccc catatcgctg
     1621 caaaccttgt cactcatggg ccgggagcac gatcacataa gctgtggcat gttactgata
     1681 aactgtaaca tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg
     1741 acataaacta tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt
     1801 cagtcagtta gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc
     1861 tggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa
     1921 gtcctccggt gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc
     1981 gctgatgcgt ctgggcgttt ttgtaccgac ccttaaatca ctgaagaaca gtaaaaaaaa
     2041 tacactgtca cgtactgatg ccacggaaga gctgacacgt ctttccctgg cccgtgctga
     2101 gggattcgat aaggttgaga tcaccggccc ccgcctggat atggataatg atttcaagac
     2161 ctgggtgggg atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact
     2221 gccttttgtt gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct
     2281 gcgtgagcgc atcagccctt ccctgaagcg cattgccggt accgtgattt cgttttcccg
     2341 taccgatgag aagcacaccc gggaatatat cacccatctg gtacagtcag cttactacga
     2401 tactgagcgg gatattgtgc agttacaggc cgatccccgc ctgtttgaac tgtaccagtt
     2461 tgacagaaag gtccttcttc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc
     2521 ccaggcactc tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc
     2581 gcggctgcgt gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag
     2641 acgggcaatg gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg
     2701 gcggacaaaa ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga
     2761 gagtaaggaa aatccgttgc aaccttcacc tgcggaaaaa gtcagtccgg agatggcgga
     2821 gaagcttgcc ctgcttgaga aaatgggcat cacgctggat gacctggaaa aactcttcaa
     2881 atcccgctga acataaactg tagtcagtga agagcgctcc tttactgact acagcttata
     2941 ttaaccggtg cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg
     3001 tctgtagctg agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgatgctg
     3061 aggcattgct cccttcataa aacatgactt actcactaca gcttatatac atgctccagc
     3121 ttatgttatg cctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct
     3181 caggggaggc agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac
     3241 tgccagcgtg gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg
     3301 atccctgtga ccaaccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg
     3361 catgatatag ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtactccgtc
     3421 agcttcggcc cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacggc
     3481 ccataatggt tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac
     3541 atagcttata tctgtcagcg gaaccaggcg cacttcatct tttggcgggc gtccgcgtcg
     3601 cgcacgcact ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga
     3661 ttccggcgtc agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa
     3721 cagcatatgg tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag
     3781 caggtaggcc ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc
     3841 aaaatccacc cccggcgcaa tgggtaacag ggagacgcgt tcaggtgaat tctgcagggg
     3901 aatgacattg ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta
     3961 ttctgacctg aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg
     4021 tttgcttatc acgcattatt gcgggtaaaa ccaaatacgt aaaggcgcgt tttgccgcag
     4081 ggatcctgtt ttaagccagt catggtgcgg gctgaggact ggtttgccgt ttttgggaga
     4141 attggggtaa tgccaggaca ggcagaaacg gagaaaaaat cctgcagacg cccttaaaag
     4201 acaactgaaa atttctgact ccatattcct ctggtttcag actgcttaat cacatcatca
     4261 acctgataca taacaaacag attctgttca cagaatgaac aattaagtaa atggttagat
     4321 tatgcgaaaa agtacagata tttctcacca tcgttattga ataatatgtc ttcacacaaa
     4381 caatat
//
LOCUS       DAJOTB010000261         4287 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJOTB010000261 DAJOTB010000000
VERSION     DAJOTB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.262"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="OHN28_004755"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="OHN28_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2300006.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(414..489)
                     /locus_tag="OHN28_004756"
     tRNA            complement(414..489)
                     /locus_tag="OHN28_004756"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:ggt)
     gene            complement(496..570)
                     /locus_tag="OHN28_004757"
     tRNA            complement(496..570)
                     /locus_tag="OHN28_004757"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Gly,seq:tcc)
     gene            complement(687..771)
                     /locus_tag="OHN28_004758"
     tRNA            complement(687..771)
                     /locus_tag="OHN28_004758"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Tyr,seq:gta)
     gene            complement(780..855)
                     /locus_tag="OHN28_004759"
     tRNA            complement(780..855)
                     /locus_tag="OHN28_004759"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Thr,seq:tgt)
     gene            1217..2167
                     /gene="coaA"
                     /locus_tag="OHN28_004760"
                     /gene_synonym="panK"
     CDS             1217..2167
                     /gene="coaA"
                     /locus_tag="OHN28_004760"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCQ2300007.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2196..3161)
                     /gene="birA"
                     /locus_tag="OHN28_004761"
     CDS             complement(2196..3161)
                     /gene="birA"
                     /locus_tag="OHN28_004761"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCQ2300008.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3158..4186)
                     /gene="murB"
                     /locus_tag="OHN28_004762"
     CDS             complement(3158..4186)
                     /gene="murB"
                     /locus_tag="OHN28_004762"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCQ2300009.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGRAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1113 a         1056 c          962 g         1156 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcgcgagc agcaccgccg
      181 taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc atggtcaacg
      241 tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc tttagacatc
      301 gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca ggcaacatgc
      361 ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag aagtggtgct
      421 gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta ccaactgagc
      481 tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      541 ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct gattttcatt
      601 ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac ttatcgtctc
      661 gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc
      721 tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac cggacttgat
      781 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
      841 ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt tcatgcaact
      901 aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga agaaacagcc
      961 gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat gtgctcctgc
     1021 taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca acgatagctt
     1081 cctgggagag attttttctt attattcctc cccatctggt gttaccctcc tgcccattaa
     1141 cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata gagctatgac
     1201 cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt acctacagtt
     1261 tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat cggaagatga
     1321 gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg ccgagatcta
     1381 tttacctctg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc gtcaggcagt
     1441 tttggaacag tttcttggta ccaacgggca acgcattcct tacattatca gtattgctgg
     1501 tagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat taagccgttg
     1561 gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc ctaatcaggt
     1621 tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg atatgcatcg
     1681 cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag cccctgttta
     1741 ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc agcctgatat
     1801 tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac acgatccaca
     1861 tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac cggaagactt
     1921 acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt ttacagaccc
     1981 ggattcctat tttcataact acgcgaaatt aactaaagaa gaagctatta agactgccat
     2041 gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac ctactcgcga
     2101 gcgtgccagt ttgatcctga cgaaaagtgc taatcatgca gtagaagagg tcagactacg
     2161 caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact acgcagggat
     2221 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agcaccctgt
     2281 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
     2341 cgattaataa aattatccaa cttttcccag cgcgaaagat aaggtgccaa tccttcttgc
     2401 tcgaagagtt ccagcgccgc gcgtaattca cgtatcagca tggccgcaag actattacga
     2461 tcgagattga tcccggcttc ctgcagcgtg atccaccctt gattaacgac actctcttca
     2521 acacgacgca ttgccatgtt gatccctgcg ccaatgacta tttgcgccgc atcgccggtt
     2581 ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
     2641 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
     2701 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
     2761 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
     2821 gcctgctggt attctgcaac acaggcatcg cccgatttaa gctctccgat acgatcaaga
     2881 aggtactgat tcgtggagtc aataacgggc agcacagtta cactaccgcc atccagctga
     2941 cccaatatct gttcagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
     3001 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
     3061 gcccggctca ttcctaacga ttcacccaac tgctcaccag agtgaaattc gccattcgct
     3121 aacagcgcga ttaatttcag cggaacggta ttatccttca tgaaatcgtc tccactgcgc
     3181 taacttcacc tgatgcacca ataaagcgaa cttcaggctc aagccagaca ttaaattttt
     3241 cacccacttt ttgtcttaca tgaagtgcca gttgcaccac atcttcgctt tttgcattgt
     3301 cttcattaat gaggactaac gcctgttgac ggtgcaccgc agccctaccc atttgcatcc
     3361 cttttagctg gcactgatcg ataagccaac ctgctgccag ttttactgaa ccatccgcct
     3421 ggggataatt tggtgctgtt ggaaattgtg ccagtaatac tttagccgtt tcggcagata
     3481 caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca gggagtttgg
     3541 tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt gtaggatcca
     3601 gacgagttaa gtcaccatac gtcagtacag gttgccactc ttttggcaga cgcaggccta
     3661 cggctacaat agcgaagcga tcctggtatt catgtttaaa aatactgtcg cgatagccaa
     3721 aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca acacaatcaa
     3781 cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg ataggtgatg
     3841 agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct tcctgcaaag
     3901 tgtattttac aaggcgatgc cagttttctc cggctcctac atgtaaatac cacgcatcag
     3961 gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg cgatagtctt
     4021 ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct tctgcggttg
     4081 catgctgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg tgctgagcat
     4141 tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct gctttcctga
     4201 tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat atgacgacag
     4261 gaagagttta tagaaacgca aaaggcc
//
LOCUS       DAJOTB010000262         4276 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJOTB010000262 DAJOTB010000000
VERSION     DAJOTB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.263"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            181..768
                     /gene="betI"
                     /locus_tag="OHN28_004763"
     CDS             181..768
                     /gene="betI"
                     /locus_tag="OHN28_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCQ2300010.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            782..2254
                     /gene="betB"
                     /locus_tag="OHN28_004764"
     CDS             782..2254
                     /gene="betB"
                     /locus_tag="OHN28_004764"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCQ2300011.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWIAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            2268..3938
                     /gene="betA"
                     /locus_tag="OHN28_004765"
     CDS             2268..3938
                     /gene="betA"
                     /locus_tag="OHN28_004765"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCQ2300012.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFNIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
BASE COUNT         1018 a         1148 c         1205 g          905 t
ORIGIN      
        1 acaccaccgg attgattttg tccttttccc tgctgtgtga aaggtctgtc atccagtatc
       61 cccactgtta ttgttaccat ttaaaatcaa attagtaaca attaagacac attttatatt
      121 gaacgtccaa tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcgaaa
      181 atgcccaaat tgggaatgca gtcgatccgg cgcagacaac tgatcgacgc cacgctggaa
      241 gcaataaatg aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc
      301 gtttctacgg ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc
      361 atgcgcgata tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg
      421 cagggcagtg cagagctgcg attacaggcg attgttggcg gaaacttcga tgaaacccag
      481 gtgagcagtg cggcgatgaa agcctggctg gccttctggg ccagcagtat gcatcagccg
      541 atgctttacc gtttgcagca ggtcagtagc cgccgcttat tgtcgaatct ggtgagcgaa
      601 tttcgccgcg agttaccgcg ccaacaggca caggaagcgg gctacggcct tgccgcgcta
      661 attgacggat tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca
      721 cactcgctga cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt
      781 gatgtcccga atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag
      841 cggtcgcacc ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcaggc
      901 cgccgggcgt gaggatgtcg atcgcgccgt gaagagtgcc caacaagggc aaaaaatctg
      961 ggcggcgatg accgctatgg agcgctcgcg tattctgcgt cgggccgttg atattctgcg
     1021 tgaacgcaat gacgaactcg caaaactgga aacccttgac accggaaaag catattcgga
     1081 aacctcaacc gtcgatatcg ttaccggtgc ggacgtgctg gagtactatg ccgggctgat
     1141 cccggcgctg gaaggcagcc agatcccgtt gcgtgagacg tcatttgttt atacccgccg
     1201 cgaaccgctg ggtgtggtgg cggggattgg cgcatggaac tacccgatcc agattgcttt
     1261 gtggaaatcc gccccagcgc tggcggcagg caatgcaatg attttcaaac cgagcgaagt
     1321 caccccgctt accgcgttaa agctggccga aatttacagc gaagcgggcc tgccggacgg
     1381 cgtatttaac gtgttgccgg gcgtgggcgc ggagaccgga caatatctga ccgatcatcc
     1441 gggcattgcc aaagtgtcat ttaccggcgg tgtcgccagc ggcaaaaaag tgatggctaa
     1501 ctcggcagct tcctccctga aagaagtgac catggagctg ggcggtaaat caccgctgat
     1561 cgttttcgac gatgcggatc tcgatctcgc cgccgatatc gccatgatgg cgaatttctt
     1621 cagctccggt caggtgtgta ccaacggcac tcgcgtcttc gttccgacga aatgcaaagc
     1681 cgcatttgaa caaaaggttc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga
     1741 tccgcaaact aacttcggcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta
     1801 tatcgccaaa ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcaatg tactgaaggg
     1861 ggatagcttc gataacggcg catggattgc accgaccgtg ttcaccgatt gcagcgacga
     1921 tatgaccatt gtgcgagaag agattttcgg gccagtgatg tccattctga tctacgagtc
     1981 ggaagacgaa gtcattcgcc gcgctaacga taccgactac ggtctggcgg cgggtatcgt
     2041 gacggcggac ctgaacctcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg
     2101 gatcaacacc tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg
     2161 cattggtcgc gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca
     2221 ggttgagatg gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact
     2281 acatcattat tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc
     2341 cgaacacaac cgtgctgctg cttgaagcag gcggcccgga ctatcgcttt gacttccgca
     2401 cccagatgcc cgccgcgctg gcgttcccgc tacagggcaa acgctacaac tgggcctatg
     2461 agacggaacc cgaaccgttt atgaacaacc gccgcatgga gtgcggacgc ggcaaaggtc
     2521 tgggcggttc atcgctgatc aacggtatgt gctacatccg tggcaatgcg ctggatctcg
     2581 ataactgggc acaagaacct ggtctggaga actggagtta tctcgactgc ctgccctact
     2641 accgcaaggc cgagactcgc gatgtgggcg agaacgatta ccacggtggc gatggcccgg
     2701 tgagcgtcat cacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg
     2761 gcatgcaggc aggctaccca cgcacggacg atctcaacgg ctatcagcaa gaaggttttg
     2821 gcccgatgga tcgcaccgtc acgccgcatg gccgtcgcgc cagcaccgcg cgtggctatc
     2881 tcgatcaggc caaatcgcgc cctaacctga ccattcgtac tcacgccatg accgatcaca
     2941 tcatttttga cggcaaacgc gcggtgggcg tcgagtggct ggaaggcgac agcactattc
     3001 cgacccgcgc ggcggcgaat aaagaagtgc tgttatgtgc aggcgcgatt gcttcaccgc
     3061 agatcctgca acgctccggc gtcggcaacg ctgaactgct ggcggagttt aatattccgc
     3121 tggtgcatga attacccggc gtcggcgaaa atcttcagga tcacctggag atgtatctgc
     3181 aatacgagtg caaagaaccg gtttccctct accctgccct gcagtggtgg aatcagccga
     3241 gaatcggtgc ggagtggctg tttggcggca ccggcgttgg tgccagcaac cactttgaag
     3301 caggcggatt tattcgcagc cgtgaggaat ttgcgtggcc gaatattcag taccatttcc
     3361 tgccagtggc gattaactat aacggctcga acgcagtgaa agagcacggc ttccaatgcc
     3421 atgtcggatc gatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc
     3481 accagcatcc gggaattctg tttaactaca tgtcgcacga gcaggactgg caggagttcc
     3541 gcgacgcaat tcgcatcaca cgcgagatca tgcatcaacc cgcgctggat caataccgtg
     3601 gccgcgaaat cagccctggt gtcgaatgcc agacggatga gcagctcgat gagttcgtgc
     3661 gtaaccacgc cgaaaccgcg tttcatccgt gcggtacctg caaaatgggt tatgacgaga
     3721 tggctgtggt tgacggcgaa ggccgcgtac acgggctgga aggcctgcgc gtggtggatg
     3781 cgtcaattat gccgcaaatt atcaccggca atttgaacgc cacgacgatt atgattggcg
     3841 agaaaatagc ggatatgatt cgtgggaagg aagcgttgcc gaggagcacg gcgggatatt
     3901 ttgtggcaaa tgggatgccg gtgagagcga aaaaatgagt cgtgatgtga actgactcag
     3961 gaacctatca gattgagagt taccgttcta ctatgaggac cgaatcctta tataaaacac
     4021 tgaggtaact ctcatgcttc atcgtgacaa tctcgccagc aaattctaag ctgacttgct
     4081 caatctggct gatgaatgtg ctatgcatac acagataaac tacagatgaa ataccactca
     4141 gcttaagaat tacgccgcta atgttcctgg ctatggttac ctgttcattc ccgctcacgg
     4201 ttagcatata cgaaaagggt tcttgtaacg agtacaaaaa taagtcagac caaaaccaca
     4261 tggagcttta aacacg
//
LOCUS       DAJOTB010000263         4196 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJOTB010000263 DAJOTB010000000
VERSION     DAJOTB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.264"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            283..1032
                     /locus_tag="OHN28_004766"
     CDS             283..1032
                     /locus_tag="OHN28_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HCQ2300013.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSDNILLSIYFQSPVILKKIQTFNGKIKFRQFVRDLLDKLCMLDSDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            959..3081
                     /locus_tag="OHN28_004767"
                     /pseudo
     CDS             959..3081
                     /locus_tag="OHN28_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3251..>4196)
                     /locus_tag="OHN28_004768"
     CDS             complement(3251..>4196)
                     /locus_tag="OHN28_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCQ2300014.1"
                     /translation="QIGDAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTK
                     LTLVTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNF
                     QVVGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLD
                     EVREEMRSLGSYIALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKA
                     RALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYK
                     TRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1247 a          964 c          908 g         1077 t
ORIGIN      
        1 gaactatatt tattctgcta caatcaatgc atacataaca caaatatcac tcaggtacac
       61 tactcaaacc acgctgggat ttttcctcaa gttataatca tcaccccgaa aatcattcgg
      121 catttactca ttaaatagtc accccatagg cctgtacatg tttactcaga aatatacatc
      181 cttttctctg tcataaaccc tctgattaat cataaataaa tacttgtgac accaatcttt
      241 ttccttaacg gaacgaattg ttgtgtagaa ggagataata ttatgtcaaa aaaatatcag
      301 ccattgctta taactcatta tatgtcaaca tgggtcacta taacggaagc agttgagatc
      361 actaccaaag ccataaaaca aaaaattact cctagcgata tttatcgtca tgccttgagt
      421 gacaacatcc tactatcgat ctatttccag tctcctgtga tacttaagaa gatacaaact
      481 tttaatggaa aaataaaatt caggcagttt gtgagagacc tacttgataa actatgcatg
      541 cttgacagtg acggttttat ttatggtcaa aatttaagac tctgtacaga agccagatat
      601 atttgtcctg tgcaacaaat tatagatacc cccctgttac gcaaactcaa ccagtttcgt
      661 acgtttgttc gtaatgtccg cccgggtgat gaactggatg tccaggcaca agttagtgaa
      721 aaaaatttaa ccccgccgcc gggtaacagc agtggcaacc ttgagcaaca gatagccagt
      781 acttcacagc taattggatc tctgctcgcc gaagatatga acagcgagca agcggcaaac
      841 atagcacgtg gatgggcctc ttctcaggct tcaggggtaa tgacagactg gttaagccgc
      901 ttcggtaccg caagaatcac gctgggcgtg gatgaagatt tcagcctgaa gaactcccgt
      961 gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca gtgaactatg tgccgaacgt
     1021 cacgaatgct gaaatcacgc tggcagcctc gaaggatccg gtgattgccg acaataacga
     1081 tctcacgaca ctaacagcac cgtcgctgat acagagggca atgcgatagc caacactgag
     1141 gtaacattta ctctaccgga agatgtgaag gcgaacttca cgctgagcga tggcgacaaa
     1201 gcggttactg atgccgatgg caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct
     1261 cataccgtga cagcatcgat ggttggcggt aagagtgagc agttggtggt gaactttact
     1321 gcggatacgc tcactgcgca ggttaatctt aacgttaccg aggacaattt catcgccaat
     1381 aacattggaa tgaccaggct acaagcaaca gtgactgatg gaaacggcaa cccagtagaa
     1441 ggtataaaag ttaatttccg tggaacctcc gtcacgctaa gcagcaccag cgttgaaacg
     1501 gatgatcagg tttttgctga aattcttgtg acaagcaccg aggtcggact aaaaacagtt
     1561 tcagcctctc tggcagataa acctactgaa gtcatctcgc gattactgaa tgccaaggta
     1621 gatgttaatt ctgcgacgat taccagtcag gagatacctg aaggtcaggt catggtcgca
     1681 caagacatag cagttaaagc tcacgttaac gaccagtttg gcaacccggt gacgcatcaa
     1741 cccgcgacat tcagtgcagc gccatcctcg caaatgatca tcagccagaa cacggtctct
     1801 actaatacgc agggagtagc cgaggtcacc atgacgtctg aaagaaacgg ctcgtatact
     1861 gtaaaagcat ccctagcgaa tggagcttca cttgagaaac aactggaggc tattgatgaa
     1921 aaactgacac tcacgtccag tccgcttatc ggtgtcaatg ctcctaaagg cgctactctg
     1981 acggcaacgc taacctctgc aaatggcact ccagtggagg gtcaggtcat caactttagc
     2041 gtaacgctag aaggtgcgac gttaagtggc ggaaaagtga gaaccaactc ttcagatcag
     2101 gctccagtcg ttctgaccag caataaagtc ggtacatata cggtgactgc atcgttccat
     2161 aacggcgtca caatacagac acagacaacc gtgaaggtta ctggcaaccc aagcaccgca
     2221 catgttgcta gctttatcgc tgatccgtcg actatcgccg ccaccaacag tgatttaagt
     2281 accttaaagg caacggttga ggatggcagt ggtaacctga tcgaaggtct cactgtgtac
     2341 ttcgccttaa aaagcggctc caccacatta acgtcattaa cagccgtgac cgatcaaaac
     2401 ggaatcgcga caacaaacgt gagatgagcg ataacaggga gcgtcactgt aagcgcagtc
     2461 ataagcgcag gtggaatgca aacagtagat atatcgctag tggcaggccc agcagacgcc
     2521 tcgcagtcca tccttaagaa caaccagtca tcactgaaag gggactttac cgatagtgct
     2581 gagctacatc ttgttctgca cgatatatca ggcaatccga tcaaagtttc ttaagggata
     2641 gaatttgtgc aatcaggtac aaacgtgccc tatatgaaaa ttagcgcaat tgattacagt
     2701 caaaatatca acggtgatta caaagctact gttacaggag gcggagaggg tatcgcaacg
     2761 ctgatccccg tattgaatgg tgttcatcag gcgggtctga gtaccacgat agaattcatt
     2821 agcgcggaaa ccagaccaat gacgggtaca gtgtcagtga atagtgcaaa cctaccgaca
     2881 gcttcattcc cttcgcaggg atttaccggg gcgtattatc agttgaataa tgacaacttt
     2941 gcaccaggaa aaacggcggc tgattattcg ttttcaagct cggcctcctg ggtaggcgtt
     3001 gatgctactg gtaaagtgac attcaaaaat gacggtgata gcaatactgt aataatcaca
     3061 gcgcccccaa gatccggtgg tgctatttac caaacagttc ctcctgaatc gcgttcggtg
     3121 tgactatgat cattttcggt gagtattgct ttgatccaaa ggctggtttt ctgtgatgat
     3181 tgaccagcct cagagtattg atgacaccac cgttcataac agatcggcat tactatgcat
     3241 aagtactgcc tcaaattctc gccaaaacac tttttaatgc ctttgcgaat tcatccagac
     3301 ttatattgtc aattttttgc ccaacgacga ctgactccga aacatcggcc attgcgggcc
     3361 atcgcgtttt gtaatcagca taaattgcaa caacaggggt attataggca cttgccatat
     3421 gtaatattga ggtgtcagga gttattgcaa tataagcatc ttttatgatt gctgctgaac
     3481 gtttaatcga gggtgataag gataaacggt agacattatt ataacaatcc actaatgccc
     3541 tggctttatc ttcccctttg ggtccataaa cgatcactat tggaatatct gtttcacttt
     3601 gaatttttgc tattagattt tctgcaatcg ataatgaaaa tgtacgttcc tgcgagctac
     3661 cttctaaatt gagcgcaatg taagatccta acgatcgcat ttcctcgcgt acctcatcga
     3721 gtacatcatc gcttaatggc aattcatata ttgacctgac tgccggaaaa cccgcctcac
     3781 gcatcataaa tgcccatgtc atgggtacag gagcccggag attctgatcc atccgggacg
     3841 cgttcttaca caaaggggaa tagcacttca tggttaaacc gacgacctgg aaattcgttt
     3901 ttgctttcaa ctgactgata aatatcatcg ttttcaggtt ctttttacgc atcgcctcta
     3961 tacaaagatc cggcgtacca tattgcagtg ttatgttctt cgctaaatct tttatttctt
     4021 ttaatgttgc gtgatcctgc atagtcattg tgactaatgt taatttagtc tgttcaagtt
     4081 tgagcgcatt aaagacttct aaattaattg tcgacgtaac aattaataga tgcttaattt
     4141 tatgcaattc aagcgcccga ataacaggaa agatggccat agcatcgcca atctga
//
LOCUS       DAJOTB010000264         4176 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJOTB010000264 DAJOTB010000000
VERSION     DAJOTB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.265"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            323..1639
                     /gene="shiA"
                     /locus_tag="OHN28_004769"
     CDS             323..1639
                     /gene="shiA"
                     /locus_tag="OHN28_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCQ2300015.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1741..3195
                     /gene="amn"
                     /locus_tag="OHN28_004770"
     CDS             1741..3195
                     /gene="amn"
                     /locus_tag="OHN28_004770"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCQ2300016.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            3538..>4176
                     /locus_tag="OHN28_004771"
     CDS             3538..>4176
                     /locus_tag="OHN28_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2300017.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLE"
BASE COUNT         1067 a          949 c          969 g         1191 t
ORIGIN      
        1 aaaaacataa ctaacctcgc ctccctactg gttataatgc aatgcagtct atcagaatca
       61 tcagggtgca attttgtgca tatgcggact tttatgtttc atatctcgaa cctgtgggcc
      121 ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc atcgaatttt
      181 tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg aggtagtctc
      241 ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact aattataaaa
      301 agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc ccgatgaagg
      361 gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt
      421 cgactggtat gattttctac tctatggcat taccgccgca ctggtgttta atcgcgagtt
      481 tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgccacct ttggtgtcgg
      541 cttccttttc cgaccgctcg gcggtgtcat tttcggtcac tttggcgacc gactgggacg
      601 taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct taattggtat
      661 tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga cactgcgtgc
      721 cattcaggga tttgccgtcg gcggcgaatg gggaggcgca gcgttgcttt ccgttgaaag
      781 tgcgccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct acggtgtagg
      841 tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg acgaacagtt
      901 tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggtac tgggagcatt
      961 gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac ataatcaagc
     1021 ggccgcgaaa aaacgcatcc cggttatcga agctctgtta cgacatcccg gtgctttcct
     1081 gaagattatt gcactacgac tgtgcgagtt gctgacgatg tacattgtta ctgcctttgc
     1141 acttaattat tcaacccaga atatggggtt accgcgcgaa cttttcctta atattggttt
     1201 gctggtaggt ggattaagct gcctgacaat tccctgtttt gcctggcttg ccgatcgttt
     1261 tggtcggcgc agggtttata ttacaggcgc gttgatcgga acgttgagcg catttccttt
     1321 ctttatggcg cttgaagcac aatctatttt ctggatagtt ttcttctcca taatgctggc
     1381 aaacattgcg catgacatgg tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg
     1441 tgccagttat cgctacagtg gtgctggagt cggttatcag gttgccagtg tggttggcgg
     1501 tggatttaca ccttttattg ccgctgcact catcacttac tttgccggga actggcatag
     1561 cgtcgccatt tatttgctgg ctggatgcct gatttctgca atgaccgctt tattgatgaa
     1621 agacaatcaa cgctcgtgat agcctggcga agatcatccg atcttcgcct tatacttttg
     1681 tttcacattc ctgtgacata ctatcggatg tgcggtaatt gtatggaaca ggagacacac
     1741 atgaataata agggctccgg tctgacccca gctcaggcac tggataaact cgacgcgctg
     1801 tatgagcaat ctgtagttgc tttacgcaac gccattggca aatatattac aagtggcgaa
     1861 ttacctgatg aaaacgcccg caaacaaggt ctttttgtct atccatcact gaccgtaacc
     1921 tgggacggta gcacaactaa tccccccaaa acgcgcgcat ttggtcgttt tacccacgca
     1981 ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct caatgaacaa
     2041 cttacgttgc tgtatcagga ttatggtgcg catatctcgg tgcaaccctc gcagcatgaa
     2101 atcccttatc cttatgtcat cgatggctct gaattgacac ttgatcgctc aatgagcgct
     2161 gggttaactc gctacttccc gacaacagaa ctggcgcaaa ttggcgatga aactgcagac
     2221 ggcatttatc atccaacgga attctccccg ctatcgcatt ttgatgctcg ccgggttgat
     2281 ttttccctcg cacggttgcg ccattatacc ggtacgccag tagaacattt tcaaccgttc
     2341 gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg atgcagccag
     2401 atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa ctggatcact
     2461 gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa acaccagatg
     2521 ccagcatggc atttaattac cgctgatggt cagggtatta ctctggtgaa tattggcgtg
     2581 ggaccgtcca atgctaaaac catctgcgat catctggcag tgctacgccc ggatgtctgg
     2641 ttgatgattg gtcactgtgg cggattacgt gaaagccagg ccattggaga ttatgtcctt
     2701 gcacacgctt atttacgcga tgaccacgtt cttgatgcgg ttctgccgcc cgatattcct
     2761 attccgagca ttgctgaagt gcaacgtgcg ctttatgacg ctaccaagtt agtcagcggc
     2821 aggcctggtg aagaagtcaa acagcggtta cgtactggca cggtggtaac cacagatgac
     2881 aggaactggg agttacgtta ctcagcttct gcacttcgtt ttaacttaag ccgggccgta
     2941 gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg cgtgccatac
     3001 gggacactac tgtgtgtttc agataaaccg ttacatggtg agattaagct tcccggccag
     3061 gctaaccgtt tttatgaagg cgctatttcc gaacacctgc aaattggcat tcgggcgatc
     3121 gatttgctgc gcgcagaagg cgaccgactg cattcacgta aattacgaac ctttaatgag
     3181 ccgccgttcc gataataagt tatgaacctt cttatcagaa atagatgtga aggattaagt
     3241 aagacatctg gcaaatagcc tgcaataaac gcgggctgtt tgcttccttg ggcagatgcg
     3301 agttttatta tcgtcttaat gatttccaca tattaaaagc aagtatgctt tcaaaacaca
     3361 attataaaaa atcccgccaa caacattagt ttttataaaa ttaaatataa gattatggct
     3421 ttagaatatt tttatttcta atagacgaga tttttcctgt tatgatataa tatgccgaat
     3481 taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg
     3541 ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa
     3601 atttatgcaa aattcggtgt agaaatctat gctgctgcca aacaaggtga acccgatcca
     3661 gaattaaaca catctttaaa attcgttatt gaacgtgcaa aacaagcaca agttccaaag
     3721 cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag
     3781 ggacgttatg aaggctttgg tcctaatggc tcaatgatta tcgctgagac attaacttca
     3841 aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc
     3901 ggagcggcag gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga
     3961 acagaccctg accatatttt tgaaattttg cttgaagctg aagttgatgt tcgtgatgta
     4021 actgaagaag aaggtaatat tgttatttat actgaaccta ctgaccttca taaaggaatc
     4081 gcggcgctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct
     4141 caatctgaag ttgagctttc cccagaagat ttagaa
//
LOCUS       DAJOTB010000265         4156 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJOTB010000265 DAJOTB010000000
VERSION     DAJOTB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.266"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2030
                     /locus_tag="OHN28_004772"
     CDS             <1..2030
                     /locus_tag="OHN28_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2300018.1"
                     /translation="SNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHIN
                     TSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIA
                     RGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYS
                     SVPVLQRAGYTRYALAMGEYRSGNNLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQ
                     AFNLGIGKDLGLFGAFSFDITQANTTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGY
                     RYSTQGFYNLSDSAYSRMSGYTVKPPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQL
                     GHYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLA
                     FTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQV
                     GNTQGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFG
                     QPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNV
                     ELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVA
                     ENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            2044..2574
                     /locus_tag="OHN28_004773"
     CDS             2044..2574
                     /locus_tag="OHN28_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2300019.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHTGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            2587..3090
                     /locus_tag="OHN28_004774"
     CDS             2587..3090
                     /locus_tag="OHN28_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2300020.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYIWQ"
     gene            3150..4064
                     /locus_tag="OHN28_004775"
     CDS             3150..4064
                     /locus_tag="OHN28_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2300021.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVILPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
BASE COUNT         1198 a          881 c          942 g         1135 t
ORIGIN      
        1 aatccaacta tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactgc
       61 gcgataactc aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat
      121 ggcagcatat caatacgtcg gctgaacgtg acattattcc gttacgctca cggttaacag
      181 tgggtgatag ttataccgat ggcgatattt ttgacagtgt gaactttcgt ggccttaaaa
      241 taaattcaac agaagcgatg ttgcccgata gccaacatgg tttcgctccg gtgattcatg
      301 gtattgctcg tggcaccgca caagtgagtg taaaacagaa tggatacgat gtttatcaga
      361 ctactgtccc acccggccct tttactattg atgatatcaa ctctgcggcc aatggtggtg
      421 atctgcaagt aaccataaaa gaggcagacg gcagtattca gacattatat gttccttatt
      481 cgtctgttcc ggttctccaa cgtgctggat atacgcgtta tgcacttgcc atgggggaat
      541 atcgtagtgg gaataacctg caaagctccc ccaagttcgt acaagcaagc ttgatgcatg
      601 gtctgaaagg aaactggaca ccttatggcg gaatgcaaat tgcagaagat tatcaggcct
      661 tcaaccttgg tattggtaaa gatttaggac tttttggtgc cttttctttc gatatcacgc
      721 aggccaatac gacgcttgca gatgacaccc gtcacagcgg acaatcggtt aaatccgtct
      781 acagcaaatc cttctaccag acgggaacca atatccaggt cgcaggatat cgctattcta
      841 cgcaaggttt ttataactta tccgacagtg catacagtcg aatgagtggt tacaccgtca
      901 agcctcctac cggagacacc agtgagcaga cactatttat tgattatttt aatctgttct
      961 acagtaagcg tggtcaggaa caaataagca tctctcagca gcttggacat tacggtacga
     1021 catttttcag tgccagtcgc caaagttact ggaacacgtc acgcagcgac cagcaaatat
     1081 catttggatt aaatgtgccg tttggtgata ttacgacttc gctgaattac agctattcca
     1141 ataatatatg gcaaaacgat cgggatcatt tactcgcttt tacacttaat gttcccttta
     1201 gtcattggat gcgtacagac agtcagtcgg catttcgtaa ttcaaacgcc agttacagta
     1261 tgtcaaacga tttgaaaggc ggcatgacca acctatcagg ggtttatggc actctgctgc
     1321 cggataataa cctgaattat agcgttcagg tcggtaacac ccaagggggt aatacatcgt
     1381 ctggcaccag tggttacagt tctcttaatt atcgtggagc ttacggtaat acgaatgtcg
     1441 gttacagtcg gagtggtgac agcagccaga tttattacgg aatgagtggt gggattattg
     1501 ctcatgctga tggcatcacc tttggacagc cgctgggcga cacaatggtt ctggttaagg
     1561 ctcctggcgc tgataatgtc aaaatagaga accagaccgg aattcatacc gactggcgtg
     1621 gctatgccat attaccattt gcgacagaat atagagaaaa tcgtgtcgct cttaacgcga
     1681 attcccttgc agataatgtt gaactggatg aaaccgtagt cactgtcatc ccaactcacg
     1741 gtgctattgc cagagcaaca tttaatgcac aaatcggcgg gaaagtatta atgacgttga
     1801 agtacggtaa taaaagcgtt ccattcggtg caattgtcac tcacggagag aataaaaatg
     1861 gcagcattgt cgcggaaaat ggtcaggttt atctgactgg acttccacag tcagggaaat
     1921 tacaggtttc atggggcaat gataaaaact caaactgtat tgtcgattac aagcttcctg
     1981 cagtctctcc tggaaccttg ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag
     2041 attatgaaat acaataacgt tattttcctc ggtttatgtc tggggttaac cacctattct
     2101 gctttatccg cagatagcgt tattaaaatt agcgggcgcg ttctcgatta tggctgcaca
     2161 gtctcatcgg attcgcttaa ttttaccgta gatctccaaa aaaacagtgc cagacaattt
     2221 ccaacgaccg gtagcacaag tcctgccgtc ccttttcaga ttacgttaag tgaatgcagc
     2281 aaagggacaa cgggggtgcg ggttgcattt aacggtattg aggatgcaga aaataatact
     2341 ctgttgaaac tggatgaagg aagcaatacg gcctccggtt tgggtataga aatactggac
     2401 ggaaatatgc gtccggtgaa attgaatgac cttcataccg ggatgcagtg gatcccattg
     2461 gtaccagaac agaacaatat tttgccttac tccgctcgtt tgaagtcaac tcagaagtcc
     2521 gtcaatccag gactggtgag cgcttcggca acctttaccc ttgaatttca ataatcagag
     2581 tcagatatga aaagacttca caagaggttc ctgttagcta cgttttgcac gttatttaca
     2641 gcaactctcc aggccgccga tgtcactatc actgttaatg gtcgggtagt cgctaaacca
     2701 tgcactattc aaaccaaaga agctaacgtt aatctcgggg atctttatac acgcaatctg
     2761 caacaacctg gttctgcatc tggctggcac aatattactt tgtcattaac cgattgtccg
     2821 attgaaacaa gtgcagtgac ggcaatcgtg acaggttcaa ctgacaatac gggttattac
     2881 aaaaatgaag gtactgccga aaatattcag atagagctaa gggatgacca ggatgcgacg
     2941 ttaaaaaatg gcgatagcaa aacggttatt gttgatgaga tcactcgtaa tgcacagttt
     3001 ccacttaagg caagagctat cacggtgaat ggaaacgcaa gccagggaac gatcgaggcg
     3061 ctaatcaatg tgatctatat ctggcaataa catcaatttg attttatttg cctccttttt
     3121 aaatcaattt tttaacctta ggatatatca tgggtaaaac attcagtata aaggtcctgt
     3181 tcggtattta tcttctatta atggcaggca aagtttttgc ctttagttgt aatgtcgatg
     3241 gcgggtcaag tattggagca ggtacaacct ctgtttatgt taatctcgat cctgtaatac
     3301 taccgggcca gaatctggtt gtagacttgt ctcagcatat aagttgctgg aatgattacg
     3361 gcggctggta cgacactgat catataaacc tggtacaagg atcagctttc gcaggctccc
     3421 tacagtcata taaaggatca ctgtactgga ataatgtgac ctaccccttc ccgttgacga
     3481 caaataccaa cgtattggat attggtgata agacaccgat gccattaccg ttaaaactct
     3541 atattacccc agtcggagca gccggtggag ttgtcataaa atcaggggaa gttattgccc
     3601 gtattcatat gtataaaatt gccacgttag gaagcggaaa tccacgtaat tttacgtgga
     3661 atattatttc aaataacagt gttgttatgc caacaggcgg ttgcaccgtt gatagtcgta
     3721 atgtcaccgt gaatctcccg gactttcctg gaagcgcaga aatacccctt ggtgtatatt
     3781 gctctagcga acaaaagctc tcattttatc tttcgggtgc caccactgac agtgcccgac
     3841 aagtgtttgc taacacagca ccagatgcaa caaaagcatc gggcgtgggt gtctctctga
     3901 tgcgtaatgg aaaaacactg gcaacaggag aaaatgtctc cctggggaca gtaaataaaa
     3961 gcaaagtacc tctgggttta agcgcaacct atggacaaac aggaaataag gttgcagccg
     4021 gagccgtcca gtcagtcatt ggtgtcactt ttatctatga ataattgtga ttttgacatt
     4081 agtctatgcg ctccattcac gaaaattgcg cggataatta ttttgtgaag gctattatcc
     4141 tacacttgta agaaaa
//
LOCUS       DAJOTB010000266         4100 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJOTB010000266 DAJOTB010000000
VERSION     DAJOTB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.267"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..213)
                     /locus_tag="OHN28_004776"
     CDS             complement(<1..213)
                     /locus_tag="OHN28_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCQ2300022.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDI"
     gene            complement(229..2709)
                     /locus_tag="OHN28_004777"
     CDS             complement(229..2709)
                     /locus_tag="OHN28_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2300023.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(2725..3399)
                     /locus_tag="OHN28_004778"
     CDS             complement(2725..3399)
                     /locus_tag="OHN28_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2300024.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3480..4022)
                     /locus_tag="OHN28_004779"
     CDS             complement(3480..4022)
                     /locus_tag="OHN28_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2300025.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLVMRVAQD"
BASE COUNT         1156 a          994 c          756 g         1194 t
ORIGIN      
        1 aatatccgac gggctcatag gggtgatagt atttgtactc acgcatgacg aatcattaaa
       61 attatgctca tatgtcgcca tttgtgtttg agtaagtgca aaatccccct gaacaaccaa
      121 attgtgctga acagataact cactataaca tgccgcatag ctcacaggca taaccatcat
      181 cattaatata aatagcatta tgcgaatttc catagaacca cctgactatt actggcaaat
      241 ataattttta ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa gcccttgttg
      301 cttatcaatt gccacattaa ccgatggcgg tacttcattg gtgcgaataa ataactggct
      361 tccctggccg acaacaccaa tattatgacc atggatatca ttgacttcat aaccaaacgt
      421 taatggttgc ccatccgctc ttaatgcttt tataaaccat ggcttgcgct gatcggtatc
      481 aaaattaacc agtacaaccg cgccgcgata aggggcggca attttccggt tgccacgtaa
      541 ttctgcttcg ctatcgcttt gtgacacatc caacatcagg taattttccc gataaggtgt
      601 cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt gcccattgac
      661 ataagcatct ttaattcctg gcgcattcat cacagcaaac gtttcagaaa gacggtttgc
      721 cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc cagcctgtcg
      781 ataagcactc gactgactat aactgccatt cactgtcgca accggcgcgt tccaggttaa
      841 attcgcccca gctgtcgttt catttccctg atactgataa ctcaggttga ccccatagtt
      901 aaactgatcc cggcttccaa cggttcctga taatcccgta ttatttgagg caaccccctg
      961 atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg taacgtcatc
     1021 accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat gatgattctc
     1081 gtcataagca tggcttgccg cgagggtata gcttatcctt cgcaggttgt tggaataact
     1141 caactgataa tccttactgc tgccgctacg cccccagtaa tctcgccata acgtacttaa
     1201 ggacacagaa ccccagcctt ctggcaatga ctgactcata ttggcagaaa agctattttt
     1261 acggccaaaa tcgttctggt aataatcggc aatgtcatag atatcgtttt catcacggcg
     1321 ataattatct ttattgtttg cccatacgtg atcattaaat gtccgataat cacgcgacga
     1381 ataacgccag gccgccagac caaaacgtgt cgacgtttgg ctcacaaatt tgttgtaggc
     1441 aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtttactat gcgactttgt
     1501 ggcatcgacg gaaatggcac caaagcgtgt attccagcca gtccccaaag tgaacgcgta
     1561 ataattattc gcgaccatcg agccaccata cagcgttaat aagttattaa aaccatactg
     1621 ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc tacgacccgc
     1681 cgcaaaatca tatttcgaca cgccgggttg cagcatattt ggtaccgctg cataaggcac
     1741 cagataggtg gttaccgaac cgtccgcctc tttcacgcta acatcaagat ccgctccacc
     1801 accggccaac tgcaaatctg taatcgcaaa cgggcctgga ggaacctctt tctgataaac
     1861 cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc cctgcactcg
     1921 tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca accgcacacc
     1981 gctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca cacgaagcgt
     2041 gccgagaagt tgggcaaatc cacgttccag atacagggta ttgctcttcc acacccctgg
     2101 attgttattt gttttactga aactggcatc agaatgcagt tgccacccca ataaatttaa
     2161 cccgctgtta aaacgtacat atgtactctt gctattaccg gacgctttat aatcgctgta
     2221 atactgactc atataataag aggtataaaa cgcattaata ccccgctccc agttttcagg
     2281 tggtacatag ccaatttcca gttcttccac ccaggcttgc gggacactga aatcgagacg
     2341 aaaaactccg atatcccagg tatagctccc gccctgaata agttgcttaa atgttaaaca
     2401 ttgcttaccg ctggcaaagt tatcggtatt aatgcctaac cgcttgatca tttcccttga
     2461 taaacatgtt tcttgcgggt tgtctttaac aatgatctca tatttcccac gccattgctt
     2521 attgacataa atatcgatgt catactgccc cggcaagggt tgattatcat caagacgaat
     2581 atttgcaacc tgctggtctt tcattccacc tatcatgaaa tgggtatcaa aggtttcttc
     2641 agctgcataa gcttcaatgc cgatcaataa cgctacgatt gctgatgcaa gtggggtcat
     2701 tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc catggtcatc
     2761 gataatggtc agatgccagt tatttgcatt attacttttg acattaacac tctgactttc
     2821 taagggggca atcataatag tttcataatt gactttgaca ttattagctt tgacatccga
     2881 aatcgtcacc caattagctg agtcattttt tatcaccaat ccattgccac tgcgatttac
     2941 cagtaacttt ttaaaggtcg ctttatttac cggagcaata cccgctggcc ggtaaaacaa
     3001 cttaattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt ctggactatt
     3061 tggtggaata tccagaacat tcagataaaa aatactctct ttattagtgg gtagtttatt
     3121 cggcataatt ttaattttta cttgctgccc ggaatttgcc cctatttttg ccactggtgg
     3181 cgttaacatg aaaggaacct gaattttttc tggtggtaat gacgtatcgc catcatcaat
     3241 ccacgcctgc atcagcgaag aacgatttcc ctggttcatc agctgcacca tcacttcttt
     3301 gtgctctgcc gggtaaataa tacgcgtccc gtagataaca ataccggcat gtgcaggaag
     3361 gaccatagaa gaaatgagta aagctaataa tcctttcatc ttaatgcctc ttaaattaaa
     3421 aggccgccat ggggatagcg gccatgttaa ttacctgaag aatcttgcaa ttttatttat
     3481 taatcctgcg ccacacgcat aaccagggaa gataacacat cacctgaatt cactgagtta
     3541 tctgtcagtt tcaggtattt tgctttaaac ggaatggcat attgaccgtc cgctatttta
     3601 taactctcga tgttgaaagg tgcagaaaca tccaggacat cgctgttatc acctaaatag
     3661 agataataac ccacggtatc gttgctactg gctatcgcct tatcgtttcc ggttatttta
     3721 tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcggacagct aatttttaac
     3781 atgctgctgg ttacaccgac tgcatcatca taggtcgagt atttagcttt accttcaaag
     3841 acagtggttg gtacagttgg catattaata cttactgaat cgccacccgc ttcgaattta
     3901 cacggagatt ctgcaatatt ccctttaatg gttaatgttc cggtatcaac gtctgcggca
     3961 aataccccag cgctcataaa aaaagaagaa aaaacagcgg cagcaataat tgaacgtttc
     4021 ataatatgct ccttaataaa atctattttt ggttgttaag agtctgtatc tcttggaaac
     4081 aaatttctca caccaaaaaa
//
LOCUS       DAJOTB010000267         4025 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJOTB010000267 DAJOTB010000000
VERSION     DAJOTB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.268"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..369
                     /locus_tag="OHN28_004780"
     CDS             163..369
                     /locus_tag="OHN28_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HCQ2300026.1"
                     /translation="MKKKQILDFIQEIKTPCRTTDIASHFEMSAYQARHYLMCLEKEG
                     KIRRTPLRRGARTLWEVVRETENY"
     gene            complement(969..1382)
                     /locus_tag="OHN28_004781"
     CDS             complement(969..1382)
                     /locus_tag="OHN28_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300027.1"
                     /translation="MNGLKTGLGITPGEHIISADSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALTAQQKAVCVVLNPEKSHPYIEDMAMFLSPLPTVLEWHCVMCERFF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRDLE"
     gene            complement(1379..1762)
                     /locus_tag="OHN28_004782"
     CDS             complement(1379..1762)
                     /locus_tag="OHN28_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300028.1"
                     /translation="MYAKSFLAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTEPPWFEHTGEGLAEHGHECPYVRPERRETLLIKRLQQWVPDALPVVRKASWHCRQC
                     HHYYYGEQYCTHCHTGRFSEEAVAG"
     gene            complement(2144..2743)
                     /locus_tag="OHN28_004783"
     CDS             complement(2144..2743)
                     /locus_tag="OHN28_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2300029.1"
                     /translation="MKTYNANPGYEMAPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTDGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYAISYL
                     AKREQKECGIVLGCSRFPEKSHRGRPRLDSTLPGICSQV"
     gene            complement(2975..3187)
                     /gene="hha"
                     /locus_tag="OHN28_004784"
     CDS             complement(2975..3187)
                     /gene="hha"
                     /locus_tag="OHN28_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCQ2300030.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAEMEAFNSA
                     VDHRLAELTMNKLYDRVPASVWKYVT"
BASE COUNT         1179 a          946 c          850 g         1050 t
ORIGIN      
        1 tcactaacca ctcaataaaa caataaacag tcaaaaacag agcacaacca ttactaatat
       61 cctttatttt catgttgtta caatgagaaa agatgacaat gagattcaac gtaagtaagg
      121 atgtataata aaaggggtta cagctaaagg tgttaacaga agatgaagaa aaagcagata
      181 ctggatttta ttcaggaaat aaagacacca tgcagaacga ctgatattgc ctcgcatttt
      241 gaaatgagcg cctatcaggc ccgccattat cttatgtgtc ttgaaaaaga ggggaaaata
      301 aggcgtacgc ctcttcgtcg tggtgcccgt actttatggg aggtagtaag ggaaacggaa
      361 aattattaat aaaaatatgc atttttcctg cataaaatta actcatccct ctactgaaat
      421 ataaccatga tttaacaacc aagaacttat attgttactt tacggcgtta tctgaaatgt
      481 tgtctaatca agatttttat accactctca gatacagatt actgatgcag tatcccatga
      541 caacgccgca tttttctgac agctgacctc ttcggcctca ttagctgtga tgtcttgata
      601 aacaaccacc gaaaaaaaga tagtcaattc gtgtaatcgt actgaagaga attccgtata
      661 aatattcagc tgacaaaacg ccatcagtcc ataaaaatca acagaagcac agatgattca
      721 tccagtcatc gacgaaaaat tgttgccata accagtggct atggtcattc tgcgcaactt
      781 cgttaaatac ccaaaaaatt tgtccgcagg tgatgaagtt cgtcaatgtc tctcttgtct
      841 gagtggtacg taaaacagat cgccagcaga tgcagccagt gatccaccgt ttgcggaaca
      901 aaacgccgga ccgtaaggct ccggcataag cgttcctgtc atgataaagt cattatgttg
      961 cgtcctcctc actccaggtc ccggagcaaa tctggctggt acctgtaaac agactttgta
     1021 cataccgctc tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa
     1081 aacctctcgc acatcacaca atgccactcc agcacggtcg gtaatggcga taaaaacatc
     1141 gccatatcct caatgtaagg gtgggacttt tccggattca gcaccacaca gaccgccttc
     1201 tgttgcgcag tcagggcatg taaatcgtgc tcaaaccacg ccccctgagc atctgtctgc
     1261 aaaatcaacc gaccacgaca ggaaaggcag aaacaatgcc tgatatttct gctgagcgca
     1321 gaatcagcac tgataatgtg ctcacccggc gtgatcccca gccccgtttt tagaccgttc
     1381 atcctgccac tgcctcctca ctgaaacgcc ctgtatgaca gtgtgtgcag tactgctccc
     1441 cataataata gtggtgacat tgtctgcagt gccagctggc tttacgcacc acgggtaagg
     1501 catccggtac ccattgctgt aagcgtttaa tcaacagtgt ttctctgcgc tccggtctga
     1561 cataagggca ctcatgaccg tgctcagcca gcccttcgcc agtgtgttca aaccagggtg
     1621 gttcggtatc gtattgcggg tggtatctga gcgcactgcc gcaaaggtga caggtgtatc
     1681 gatcgtaagg cgctgtctgt gcagtgcggg cgccggtcag gcgtccgttg ccatcgaatg
     1741 cgagaaaaga ttttgcgtac atcgtcatat tcctcagccg ccagacgaca cgcaggcagc
     1801 aaccgtcccg gtacgggcag cgtggtcagg gtgtgaatgg cggttcagtt aaaaatctgg
     1861 aatggggtag aggtaaaaca ggcaggctgt tgttacaaca ctgtggttgt cacacaatgg
     1921 cccaccgacg taaaaaactg tcagcaagca gagatggaca tccagcgcat gcagcactgt
     1981 tgcagccatc aggggtttta ctggtgtgtc atgtatggtt aagtttccgt atggattcgt
     2041 tcatacggag taatcacatc gtaaaaaatg cccagtgcct ctgaaatagc ctccggaacc
     2101 gactgcaaag attagagcca gtccgcagtt gtcgctcaga accttatacc tgagaacata
     2161 tccccggtag ggtgctgtcc agtcgcggtc gaccccggtg acttttttca ggaaacctgc
     2221 tgcaaccaag aacgatgcca cattctttct gttctcgttt ggcaaggtag ctaatggcat
     2281 agcgtaaagc attcaccgct ttgtggtctg aatagtgaat gacgtggtta atgtttaccg
     2341 gataattttt attaaaccgg caccagtggt agtaaccatc gccatcggta acccgcctcc
     2401 agatatctcc catcaatctg gataccagat acgaactcct gtggctctga ccgttcagat
     2461 agcccacaaa atgaatgtga agccctttac gttccgtata ttccattacc caggcatacc
     2521 cgaccagttt ttctcccacc tcgttaatca gtcgcaacat ctccgcgcac attgcgtacc
     2581 tgcaggcaca cctgtagctt agggtgtttt ttcggtaagc aaaatccatt ctgaacggta
     2641 acagtttcga atataccccg aataatgaat ccagatgctg gttaaagtgt gtcaggagct
     2701 ggggagccat ttcgtaaccg ggattggcgt tgtaagtctt catgtttctt cctcgtgttc
     2761 tgtaacacag gaagaagcgc tggcgtaatt tttgaagaaa acgttatcat acgaagacgt
     2821 gcaagttcac aactccccgg gcgccgcaga gaaagtggga ccactcccgg gtatcagaac
     2881 catacagaga acggcatacg tgacagtcaa tcacagcgca gataatatct gatgtgatgt
     2941 cattgtgacg actgtacttc atcagaaaca tttatcaggt aacatatttc cagactgaag
     3001 cgggaacacg atcgtaaagt ttgttcatgg tcagttctgc cagccggtga tcgaccgcag
     3061 agttgaaggc ttccatctcc gctggagtaa gcttataacg cgtgtgggaa atcacttttt
     3121 ccagtgtctc ccgggatgaa caacgacgga actgatacag ccagtcttct ttggtcttaa
     3181 cttccattat cctctcgtta ttttgtgctg tgattaacgt taacataata ccgtcagaat
     3241 aacgtattct gacgtcatcc cggaagtctt tccactttcc tgcctctccg gtaataaaac
     3301 attttaaatg ctgaaggcat aacgccataa ccagatatta caagtaacgt ggcgttacac
     3361 gtattaatgc attacagaag taacagagac aatcagatag taaacaacac tccggaagac
     3421 tgtttaatgg cataaggtat actttttacg atcacaatat tatattacta ccaaaattca
     3481 tcaggtgaac tgcaaaagca aaacattaac agttctccct caaccaccgt tatccacact
     3541 attgacactg acgattcata ctcttattta gccagaatag gctccacaca acaggtagaa
     3601 taacagacat tacttaacaa agtacgttac agtcagtgtg tacattacag atttatattc
     3661 aggtattgcg ggactaatta ctgaagagag agttaaggac agagttacag tactactaca
     3721 gagaatatca ctgcacagat acttctatga acatacttct atgagataga cttttacagg
     3781 attgggttac aaggtacatt tacagggtgc atttacataa ccatacttct ataaataata
     3841 ttaattaacc ttaattattt tttaatgata catgaactgc ctgtagctgt aacgataaac
     3901 aaattaccca aaacgaaaaa tactggttat cacggttcag agaactcgta taagtacctc
     3961 ccccctgagc aatgcttcat tttctgtgat tctatgtgat agagttatgc tgaatacagt
     4021 ggcag
//
LOCUS       DAJOTB010000268         4013 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJOTB010000268 DAJOTB010000000
VERSION     DAJOTB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.269"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            7..1665
                     /gene="dpiB"
                     /locus_tag="OHN28_004785"
     CDS             7..1665
                     /gene="dpiB"
                     /locus_tag="OHN28_004785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCQ2300031.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1634..2314
                     /gene="dpiA"
                     /locus_tag="OHN28_004786"
                     /gene_synonym="citB"
     CDS             1634..2314
                     /gene="dpiA"
                     /locus_tag="OHN28_004786"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCQ2300032.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2355..3740)
                     /gene="dcuC"
                     /locus_tag="OHN28_004787"
     CDS             complement(2355..3740)
                     /gene="dcuC"
                     /locus_tag="OHN28_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCQ2300033.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1097 a          941 c         1034 g          941 t
ORIGIN      
        1 aaaacaatgt tgcagcttaa cgagaataaa cagtttgcat ttttccaaag actggcattt
       61 ccgctgcgta tctttttgct gattctggtg ttctcaatat ttgtcattgc agccctggcg
      121 caatatttta cggccagttt tgaggactat ttaacgcttc atgtgcgcga catggcaatg
      181 aatcaggcaa aaattattgc ttctaatgac agtatcatca gcgcagtgaa aacgcgtgac
      241 tacaagaggc tggcgaccat cgctgacaaa ttacaaagag atactgattt cgattatgtg
      301 gtgattggcg accggcactc gattcgtctt taccatccta atccggagaa aattggttat
      361 cctatgcagt tcaccaaacc gggcgcgctg gagaaagggg agagctactt catcaccggg
      421 aaagggtcaa ttggcatggc gatgcgtgcc aaaacgccaa tctttgatga cgatggaaaa
      481 gtcatcggtg tggtgtcgat tggctatctg gtgagtaaaa tcgatagctg gcgggctgag
      541 tttttattac cgatggcagg cgtgtttgtc gtgctgttag ggattttgat gttgctatcg
      601 tggttcctgg ccgcgcatat ccgtcggcag atgatgggca tggagccaaa gcaaatcgca
      661 cgcgtggtcc gtcagcaaga ggcgctgttt agttcggttt atgaagggct gattgcggtg
      721 gatccgcacg ggtacattac cgccatcaat cgtaacgcaa gaaagatgct ggggctgagc
      781 tcccccggac ggcaatggtt gggtaaaccc attgctgaag tggtcaggcc cgccgatttc
      841 tttaccgaac agattgatga aaaacgtcag gatgtggtgg cgaactttaa cggtctgagc
      901 gttatcgcca atcgggaagc tattcgttct ggcgatgatt tgctgggggc cattatcagc
      961 tttcgtagta aagacgaaat ttccaccctc aatgcgcaac tgacgcaaat taaacaatac
     1021 gtcgagagcc ttcgtacatt gcgacacgag catctcaatt ggatgtcgac gctcaatggt
     1081 ctgttgcaga tgaaagagta tgatcgcgtg ctggcgatgg tgcagggtga gtctcaggcc
     1141 cagcaacagc ttattgacag cctgcgtgag gcgtttgccg atcgccaggt ggcggggctg
     1201 ctttttggta aagtgcagcg cgcccgcgaa ctggggctaa aaatggtcat cgtccccggt
     1261 agccagcttt cgcaactgcc gccaggactg gacagcaccg agtttgcagc cattgttggc
     1321 aatttacttg ataacgcctt cgaagcaagc ctgcgtagcg atgaaggaaa caagattgtt
     1381 gaattatacc tcagcgatga aggcgatgat gtggtgattg aagtcgccga tcagggctgc
     1441 ggcgttccag agtctctacg agacaaaata tttgagcagg gtgtcagtac gcgtgctgac
     1501 gagcccggtg aacatggcat tgggttgtac ttgattgcca gctacgtaac gcgctgcggt
     1561 ggtgttatca ctctcgaaga taatgatccc tgcggtacct tattttcaat ctatattccg
     1621 aaagtgaaac ctaatgacag ctccattaac cctattgatc gttgaggacg aaacgccgct
     1681 ggcagagatg catgcggaat atattcgtca cattcccgga ttcagccaga tattactggc
     1741 gggaaacctg gcgcaggccc gaatgatgat cgagcgtttt aagccggggc taatcttgct
     1801 cgataactat cttcctgacg gtagagggat taatttactg catgaactgg tgcaggcgca
     1861 ttatcccggc gacgtggtgt ttaccactgc agccagcgat atggaaacgg tgtctgaagc
     1921 cgtacgttgt ggtgtatttg attatctcat taagccaatt gcctatgaac gtctggggca
     1981 aacgctaacc cgtttccgcc agcgtaaaca tatgctggaa agtattgata gcgccagcca
     2041 gaagcaaatt gatgagatgt ttaatgccta tgctcgcggt gaacccaaag acgaactgcc
     2101 gaccggcatt gaccccttga cgctaaacgc ggtgcgaaaa ctgttcaaag agcctggtgt
     2161 gcaacatacg gcagaaacgg tggcgcaggc actgaccatc agtcgcacca ctgccaggcg
     2221 ttatcttgaa tattgcgcca gccgccatct gattattgct gaaattgttc acggcaaagt
     2281 tggcagaccg caacgcatat accacagtgg gtgacatgat aaagccggag gaaacttgcc
     2341 tccggcatta ctatttactt gcctgtgacc gctgctgccg ttcctggcac catcagctct
     2401 gtagcaacga taacaatcac caaaccaaca agcaccggca ccgaggtgcg ttttacgact
     2461 tcaaacggcg agatcttcgc catcccggca accgcaacga ctacgccaga aacgggtgat
     2521 agggtacgac ccaggtttga cgcctgcagc atcgggatag tcaaatacgc cgggttaatg
     2581 ccggaggagt gcgccagttt cgggatcatc tcaacaaacg cataaaacgg cgcattgcct
     2641 gaaccggtcg tgactgccgc cagcattgtc aggatcacca ataccagcat caggatgata
     2701 ctcgccgaac caaacgaggt agcgatagaa atcagacttt gaataaagcc gatggtgcta
     2761 agcccctgag cgaatacccc agcggcaacc agcagcatca ccacgttagc aaatgcatct
     2821 gccataccgc gataagccac ttccagacca gagaaaactt tctgggtatt aaagctgcgg
     2881 atgaactcca gaatggaggc aattagcata caaatcacca gaatagtgat gatgtgtaat
     2941 tgcggacccc atttgccgtc aaaaatcagc actccgatga tcggcgtgaa cggcaaaatg
     3001 gcataaaacg caggggcagt ggtggtgatt tcactgacat ctaacatttc atgagagatg
     3061 tgctcttttt tatccagata acgttgccag aagaagtggg cgatcgccat gccgataatt
     3121 gcagcaattg agataggcag cgttgttttg aaggcgaagt caatcagcga catttcggaa
     3181 gcctgcgctg ccagcaccac atcccctgaa gtcggtgcga gaataatcgc cgccggggag
     3241 gcacaaatgg cagcagctgc gccacgactg ataccaacgt ttaccatcac cggaaacagg
     3301 gttgccatca gcaaaacacc cagaccggtt gcggaagaga cggccagtga catcagacag
     3361 gcaacaaaat aggcggcaat catcagaagg taaggggagt taatatactg caatggtttt
     3421 gacgccagct tgaccaccat atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca
     3481 cacagcatca taatcatcat gccgaggtcg ccgccgcggc tcattagcaa tattttaacg
     3541 tattcaacga tatccgtggc gctgtagcct gttgaagcct ggctggacgg taacactttg
     3601 tgccccataa tggcactgat aatcagcaat aacaggccac cgacaaataa cacgccagtg
     3661 gcagaatacc ctttaatgat gtagcgagct acacccacaa taaccacaac cccaataagg
     3721 agttcaatga atgtcagcat aatttttcct gtctccaggc cccaaagtaa ataataaaaa
     3781 attcctaaaa gttaaggaaa aaatatgccc aataaattgg cggtgaatgc tgattaaaat
     3841 caagaaaaac tgccattaag acattgaagt tgctgttttt atacgtagat aacaaaacgc
     3901 atatttagcg gttggcaaat atcacataat aatgtgtgta aattgatggc ggattgatgc
     3961 aaatttgtta attgtaaggt gttagctgcg gttgtgtgct caaaaaataa tct
//
LOCUS       DAJOTB010000269         3953 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJOTB010000269 DAJOTB010000000
VERSION     DAJOTB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.270"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            102..305
                     /locus_tag="OHN28_004788"
     CDS             102..305
                     /locus_tag="OHN28_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCQ2300034.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            313..1245
                     /gene="aaeA"
                     /locus_tag="OHN28_004789"
     CDS             313..1245
                     /gene="aaeA"
                     /locus_tag="OHN28_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCQ2300035.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1251..3218
                     /gene="aaeB"
                     /locus_tag="OHN28_004790"
     CDS             1251..3218
                     /gene="aaeB"
                     /locus_tag="OHN28_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCQ2300036.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPCMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            3310..3582
                     /locus_tag="OHN28_004791"
     CDS             3310..3582
                     /locus_tag="OHN28_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCQ2300037.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3637..3900)
                     /gene="yhcN"
                     /locus_tag="OHN28_004792"
     CDS             complement(3637..3900)
                     /gene="yhcN"
                     /locus_tag="OHN28_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCQ2300038.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          905 a          958 c         1060 g         1030 t
ORIGIN      
        1 tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg acagatgctg
       61 aaaataataa cgcctgctct ctctatacaa ccaaggtcaa catgagtctg tttcccgtta
      121 tcgtggtgtt tgggctgtcc ttcccaccga tatttttcga attgctttta tcactggcga
      181 ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt gtctggcatc
      241 cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg cgactgttcg
      301 tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat cacggtcgta
      361 ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac cgaatccccg
      421 tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga
      481 ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca ggtactgttc
      541 accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat
      601 tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg
      661 caggcgatgt cccgcgaaga gatcgaccag gccaacaacg tactacaaac ggttctgcat
      721 cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga acgcacggtg
      781 atccgcgcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg tgagtttatt
      841 acccgtggat caacggcggt tgcgctggtg aaacagaact ccttctatgt actggcctat
      901 atggaagaaa ctaagctgga aggggtgcgc cctgggtatc gtgcggagat cacgccgctt
      961 ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgcaggggt caccaatgcc
     1021 agcagcacgc gtgacgacaa agggatggcg accatcgact ctaaccttga atgggtacgt
     1081 ttggcgcaac gtgttccggt acgtattcgt ctcgacaacc agcaagagaa catctggcct
     1141 gcgggcacca ctgctacggt ggtagtcact ggcaaacaag atcgcgacga aagtcaggat
     1201 tcgttcttcc gtaaaatggc tcatcgcctg cgtgagtttg gttaatcacg atgggtattt
     1261 tctccattgc taatcaacat attcgctttg cggtaaaact ggcgaccgcc attgtactgg
     1321 cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta ctgacagcgg
     1381 cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct ggcgctattc
     1441 gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc ggactggtaa
     1501 tcatcattgc gatgatccga gcaccattat tgatgattct ggtgtgctgt atctgggccg
     1561 gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtatgcg tgggggctgg
     1621 ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg cttacgccgc
     1681 agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgca attatggcgg
     1741 atttgctctt ttctccgcga tcgatcaaac aagaagtgga tcgagagctg gaaagtttgc
     1801 tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt gaagttgtcg
     1861 ataaagcctg gggcgatctg gtgcgacgta ctacggcgtt acaaggtatg cgcagcaacc
     1921 tgaatatgga atcttcccgc tgggcgcggg ctaatcgacg tttaaaagcg atcaatacgc
     1981 tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat acgcgcccgg
     2041 aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaact gcgcaggacg
     2101 tccacaagca gctcaaacgc ctgcggagag tgatcgcctg gaccggggaa cgggaaacgc
     2161 ctgttaccat ttatagttgg gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg
     2221 ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc gaaccggaag
     2281 taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga accacacttt
     2341 cttgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc agtggtgcaa
     2401 tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca tgcatggtgg
     2461 cgatcgactt tatctacggg acgctggcag cgctgccgtt agggctgctc tactttttgg
     2521 tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg gcagtgctgg
     2581 gatttttcct cggcatagaa gtacagaaac ggagattggg ctcgatgggg gcgttggcca
     2641 gcaccataaa cattatcgtg ctggataacc cgatgacttt ccatttcagt cagtttctcg
     2701 acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc
     2761 gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt tctgccgctg
     2821 tttccgcgat gactaccaat gtggcacgtc gtaaagagaa tcacctcccg gcactttatc
     2881 agcagctgtt tttgctgatg aataagttcc caggggattt gccgaaattt cgcctggcgc
     2941 tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt
     3001 tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct gcccgtagcg
     3061 atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc taccaggaaa
     3121 agctacgcat ctggcaagcg ccaccgcagg tgacggaacc agtacatcgg cttacgggta
     3181 tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac gcctgaagcg
     3241 tcggtttttt catggctata cttagcgatg aacggcagaa ctcgccgcga aacgtgacgg
     3301 tggcaacaga tgaatattta tacctttgat tttgatgaga ttgagagtca ggaggatttt
     3361 tatcgtgact ttagccaaac ctttggtctg gcgaaagata aggtacgcga tctcgactca
     3421 ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga atttgttcat
     3481 ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga tgaggcagag
     3541 gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa aagcccccga
     3601 accgggggca atatcgtcgg acaagacgat gagggtttat ttgtacagtt cagccgtagc
     3661 gtgccaggtg tcaccactac gagcttcagt aatctggtag gccgttgcgc ctttctcttc
     3721 cgcttttttg ttcagcattt cacgcatatc cattggcgaa gacgccacac cacttacgga
     3781 tacggtcccg attgcttcac gattttgtgc ttgtgcagca tcaatggagt cggcagcgaa
     3841 tgcaccgaaa gagagaacag aaagtacgct taatgcagca acagtggttt tgattttcat
     3901 gatttttacc tcgacataat cttttagctg ggcctttgtt tcgtgaccca tat
//
LOCUS       DAJOTB010000270         3941 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJOTB010000270 DAJOTB010000000
VERSION     DAJOTB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.271"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..349
                     /locus_tag="OHN28_004793"
     CDS             <1..349
                     /locus_tag="OHN28_004793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300039.1"
                     /translation="EIRSELAGHLSDSEIDDSFSLLRSVKKRGFTPHLIKEKFEELKL
                     GDGTLTLNKILSCLFKVGAIGNVLRRSKTDGGDVYLWSFNDEDLEMDPTLNFEIHCGL
                     WDALGIIKPKLRQ"
     gene            476..1054
                     /locus_tag="OHN28_004794"
     CDS             476..1054
                     /locus_tag="OHN28_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCQ2300040.1"
                     /translation="MNTQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITLKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDVHQHTGVLKGGDTTGGPV"
     gene            1051..1410
                     /locus_tag="OHN28_004795"
     CDS             1051..1410
                     /locus_tag="OHN28_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCQ2300041.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            1397..2305
                     /locus_tag="OHN28_004796"
     CDS             1397..2305
                     /locus_tag="OHN28_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCQ2300042.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVM
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDHLAANYNVKRLT
                     VTPADDDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEILPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQRYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            2298..2903
                     /locus_tag="OHN28_004797"
     CDS             2298..2903
                     /locus_tag="OHN28_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCQ2300043.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            2900..>3941
                     /locus_tag="OHN28_004798"
     CDS             2900..>3941
                     /locus_tag="OHN28_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2300044.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKVLELK
                     VYADDQMAKHLAAPDPHSQYAPKESPTFTGTPKAPTPAAGNNTTQIATTAFVQAALMA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLL"
BASE COUNT          957 a          963 c         1147 g          874 t
ORIGIN      
        1 tgaaattaga tctgagcttg cgggacattt aagtgattca gaaatagatg acagtttttc
       61 tcttttacgt tcagtaaaaa aaagaggttt cactccacat ttaatcaagg aaaaatttga
      121 agaattgaag ttaggagatg gtacattaac gcttaataaa atactgagtt gtttatttaa
      181 agtcggtgct atcgggaatg tactcaggag atctaaaaca gatggcggag atgtttattt
      241 gtggtcattt aatgatgaag atttagaaat ggacccaacg ttgaattttg aaatacactg
      301 tggtttgtgg gatgcactag gaattatcaa gcctaaactt aggcaataat aaaaacgccc
      361 tttaataggg cgtttttatt taaagttgtc attctttctt taaattatca ttgtgccaaa
      421 tctcttacaa ttctaaatag atgaattagt ctttgttctg tttgataatt tggaaatgaa
      481 tacacaactg accgaaatca tgcgccttat caccaacctg atccgcactg gtgtagtcac
      541 cgaagtggac agggaaaact ggctttgccg ggtgaaaaca ggcgacttag aaaccaactg
      601 gatcagctgg ctgaccctgc gtgccgggaa tgcccggaca tggtggcgac catcggaagg
      661 tgagcaggtg gtgctgctga gtctgggcgg caatctggag accgcctttg cgctgcccgc
      721 tgtctattcg aatcagttcg caccaccgtc gacgtcggcg gacgcctgcg tgacagaaca
      781 tcctgacggt ggctggtttg aatacgaacc cgccaccggg cgctggtatg tcaggggcat
      841 caaatccatg gttattgagg ctgccgacaa tatcaccctg aaaaccagtg agtttgtgct
      901 ggaggctgac cgcacgcgta ttaatagtga agtagtgatc aatggcggcg ttacccaggg
      961 cggcggtgcg atgagttcta acgggatcgt ggttgatgtg catcagcata ctggcgtcct
     1021 gaaaggcggc gatacaaccg gaggcccggt atgacgcttt atagcgggat gaacaatacc
     1081 agcggtaaag ccattactga tattgaccat ctgcgccagt cggtgcggga cattctgctg
     1141 acaccgcagg gtagccgcat tgctcgccgg gaatatggtt ccctgctgtc ggcactgata
     1201 gatcagccac aaaatccggc attacgcctg caggtcatgt cggcagtgta tgtggcgctg
     1261 agtcgctggg agccacggct gacgctggat tccatcacca tcaacagcaa ttttgacggt
     1321 tcaatggtgg tggagctgac cgggcggcgg aataacggtg tgcctgtttc cctttccgta
     1381 tcaacaggag cagagaatgg cagtgattga cctttcgcag ttacctgcgc cgcagattgt
     1441 ggatgtgccg gactttgaga cgctgcttgc cgaacgcaag gcagaatttg tggcgcttca
     1501 tccgaaagat gagcaggaag cagtgatgcg tacgctggaa ctggaatctg aacccgtcac
     1561 caaattgctg caggagaacg cttaccgtga gttgcttctg cgccagcgca ttaacgaagc
     1621 cgcgcaggct gtgatggtgg cttacgcgat gggcggcgat cttgaccatc tcgctgctaa
     1681 ctacaacgtg aaacgcctga cggtgacacc tgctgatgat gacgctgtac cgcctgttgc
     1741 ggctgtgatg gaaagtgatg aagcgttacg cctgcgtgtg cctgcagcct ttgaagggct
     1801 ttctgttgct gggccaactg ccgcttatga gtttcatgca cgaagcgccg acggtcgggt
     1861 ggcggatgcc agtgcaacca gtccggcacc tgcagaggtg gtgcttacgg tcctaagccg
     1921 tgaaggcgac ggaacagcag aaaaagacct gctggatgtg gtggagaaag ccctgaacag
     1981 tgagaacgtc cgcccggtgg ctgaccgtct gacggttcgc agcgcggaaa tcctcccgta
     2041 ccgcgtggaa gccaccattt ttctctatcc ggggccggaa gcagagccgg taatggcagc
     2101 ggcaaaagcc agcctgcaga ggtacatcgc cagtcagacg cggcttggtc gggatattcg
     2161 ccgtagcgcc atttttgccg cgctgcatgt tgagggtgtt cagcgtgtag aactggcttc
     2221 cccgctggcg gatgtggtcc tgaacaaaac gcaggcggca tcatgtacgc agtggagcgt
     2281 gaccaacggg ggtacggatg aatagtctgc tgccaccggg ttcaacgcca ctggagcgcc
     2341 gactggcgca aacctgcagc gggatttctg atctgcaggt gccgctgcgt gacttgtgga
     2401 atccggcaac ctgtccggtc agtttcctgc cttatctcgc ctgggcgttc tctgtggatc
     2461 gctgggacga gggctggaca gaaagcgtca aacgtcaggt ggtgaaggat gctttttata
     2521 ttcatcagca taaagggacc accagtgccg tgcggcgggt ggtggagccg ttcggctttc
     2581 tgatccgcat tattgagtgg tggcagaccg gagaggcacc gggcacgttt cgtctggata
     2641 tcggcgtgca ggaccagggc atcactgaag atacctatct ggaacttgag cgactgataa
     2701 gcgatgccaa accatgtagc cgtcacatga tcggcatgtc catcaacctg cagaccagcg
     2761 gcccgcactg ggtgggagcc gccagctatc ttggcgaaga aatcacgatc tatccgtata
     2821 tcaacgaaac aattatttcc ggcggcaccg cgcatgaagg cggggcggtc catgttattg
     2881 acacaatgag agtgaatcca tgagcacaaa attttatacc ctgctgacgg atattggtgc
     2941 ggcgaaactt gccagcgccg ccgcgctcgg tgtgccgcta aaaattaccc atatggcggt
     3001 gggcgatggc ggcggagtat tgccgacgcc ggacgcaaag cagactgcac tggtaaatga
     3061 gaaacgccgg gctgcgctga atatgctcta tatcgacccg cagaacagca gccagattat
     3121 tgctgaacag gtgatccctg aaaacgaggg cggttggtgg atacgtgaag tgggcctgtt
     3181 tgatgagtca ggggcattga ttgccgtggg gaactgcccg gaaagctata aaccgcaact
     3241 ggctgaaggc agcgggcgca cccagaccgt gcgcatggtg ctgattacca gcagtacgga
     3301 caatatcaca ctgaaaattg accctgccgt agtgctggca acccgcaagt atgtggatga
     3361 taaggtactg gagctgaagg tgtacgcgga tgatcagatg gcaaaacatc ttgccgcacc
     3421 ggacccgcat tcacagtatg cacccaaaga aagtccgacg tttaccggga cacccaaagc
     3481 gccaacgcca gcagcaggga ataacaccac gcagattgcg accaccgcgt ttgttcaggc
     3541 ggcactgatg gcccttatta atggtgcgcc agccacactg gatacgctga aagaaattgc
     3601 cgctgccatt aataatgacc cgaaattcag taccaccatt aacaatgcgc tggcactgaa
     3661 agcgccgttg tcgagtccgg cactcaccgg aacgccaaca gcccccacgg cggcgcagtc
     3721 ggtcaacaat acacagattg ccactacggc ttttgtgaaa tcggcgattg cggcaatggt
     3781 gggttctgca cctgcggcac tggatacact gaacgaactg gcggcggcgc tggggaatga
     3841 cccgaacttt gccacgacaa tgcttaatgc actggcaggt aaacaaccgc tggacaatac
     3901 gctgactaat ttgagtggaa aggatgtagc tggtcttctc a
//
LOCUS       DAJOTB010000271         3857 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJOTB010000271 DAJOTB010000000
VERSION     DAJOTB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.272"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            104..961
                     /gene="focA"
                     /locus_tag="OHN28_004799"
     CDS             104..961
                     /gene="focA"
                     /locus_tag="OHN28_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCQ2300045.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1016..3298
                     /gene="pflB"
                     /locus_tag="OHN28_004800"
     CDS             1016..3298
                     /gene="pflB"
                     /locus_tag="OHN28_004800"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2300046.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3617..3835)
                     /gene="ogrK"
                     /locus_tag="OHN28_004801"
     CDS             complement(3617..3835)
                     /gene="ogrK"
                     /locus_tag="OHN28_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HCQ2300047.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
BASE COUNT          970 a          966 c          938 g          983 t
ORIGIN      
        1 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
       61 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
      121 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcag gtgtctataa
      181 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
      241 aatcgcattc gtcttctata tcacagcaac cactggcaca ggtacaatgc ccttcggcat
      301 ggcaaaactg attggcggca tttgcttctc tctggggttg attctttgtg ttgtctgcgg
      361 agccgatctc tttacttcca cagtgttgat tgttgttgcc aaggcgagtg ggcgcatcac
      421 ctggggtcag ttggcgaaaa actggctaaa tgtttatttt ggcaacctgg tcggcgcact
      481 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
      541 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
      601 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
      661 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
      721 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
      781 cgcatctccg gaattctgga ccgctgtcgg ttctgcaccg gaaaattttt ctcacctgac
      841 cgtgatgaac ttcatcactg ataacctgat tccggttacg atcggtaata ttatcggcgg
      901 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg atcaccatta
      961 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     1021 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     1081 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     1141 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     1201 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     1261 cacctctcac gacgctggtt acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     1321 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     1381 ctgcaaagcg tacaaccgcg aactggaccc gatgatcaaa aaaatcttca ctgaataccg
     1441 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     1501 atccggtgtt ctgaccggtc tgccagatgc ttatggccgt ggtcgtatca tcggtgacta
     1561 ccgtcgcgtt gcactgtacg gtatcgacta cctgatgaaa gacaaacttg cacagttcac
     1621 ctctctgcag gctgatctgg aaaacggcgt aaacctggaa cagactatcc gtctgcgcga
     1681 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     1741 ctacgacatc tctggtccgg ctactaacgc tcaggaagct atccagtgga cttacttcgg
     1801 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     1861 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     1921 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     1981 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     2041 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     2101 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     2161 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     2221 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     2281 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     2341 catgctgtac gcaatcaacg gtggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     2401 atctgagccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     2461 cttcatggac tggctggcta aacagtacat cactgcactg aacatcatcc actacatgca
     2521 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     2581 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     2641 tgcgaaagtt aaaccgattc gtgatgaaga cggtctggct atcgacttcg aaatcgaagg
     2701 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     2761 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     2821 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaactggta acaccccaga
     2881 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     2941 gaaaggtgct gtagcgtctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     3001 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     3061 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     3121 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     3181 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     3241 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     3301 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     3361 ttttttagca cgagagcctt ttttgtcagc tatctatact ttaaggtgac tgccaaaaca
     3421 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     3481 aacaccgcaa tgtcagttat tggtgacatt ttttgatatc taatcacatg aaataaaagg
     3541 atttatttct ggtcacgtcc acacattgac cacatcgaca aaaaagcccc tcgactgagg
     3601 ggctttctgt ttgtaattac atccacataa tttgctgccc tgacggcaac gggtgcggcc
     3661 tcacggcgtg gacttctccc ggcttcacga tgtatctctg taccgactca taagtgatga
     3721 acgtggcgct gcaattcacg ttctggcact ggtgataacg ctcttttgtc gtgtcagtga
     3781 tatagcggct tgtacgcgca tgtgcggcat gctggcataa aggacaatga aacatcgcga
     3841 gcacctcttc cggtttt
//
LOCUS       DAJOTB010000272         3773 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJOTB010000272 DAJOTB010000000
VERSION     DAJOTB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.273"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(653..1564)
                     /locus_tag="OHN28_004802"
     CDS             complement(653..1564)
                     /locus_tag="OHN28_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300048.1"
                     /translation="MMQSEHTAPCPTTSLSLPALLWDTRPEISESELAALDTLVDHFQ
                     QGGKNWSPDIQKRLSRLLLPLRDTLTKMHAAKAPYNSSIHDIVLEMQRIRKTYWAWTQ
                     EEWLEVICNSEGEFRRRFGARGNCRQYVIALAWLLCGFERLEHCGIFYQYRLCLKVFG
                     RQSTDFAVSQLDNMMQVLGYVPRDSRNNGIRNAMCMAMLLQRDAQLDHITVTTLQQIA
                     TTCPDYLREASATLSRILAASGTIEEGFDYRITQRRRPPREYNATADVPTKWLVWCKR
                     WRATSVLRPSSILSGWYVLLKCGQLVS"
     gene            complement(1561..2559)
                     /locus_tag="OHN28_004803"
     CDS             complement(1561..2559)
                     /locus_tag="OHN28_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2300049.1"
                     /translation="MHKKLLDALPFLSADPSACRWVCLQEDLLCAPNTIDAYARGVND
                     WLAFCHSVALTAAVAGRDTVALYVRSLHTGRQLAAATIRHRLTVVRLYNDWLCEEGIR
                     ERNPVRRGVWKNGGKGKAGIVPLQRRLPWIPDDAEWLRFLEVAGQADIRTRFMLALAY
                     DCGLRREELCTVATGDIDPSQRLLTVRAEHTKNRFGRVVPYSPVTGELYTAWLTERRM
                     LSSSRGPLFLSRSPRNRAEPISNWTWSKVVRGLAIKADLPLISTHTFRHLCLTELARV
                     GWDIHEIAAFAGHRRIQSTLLYIHLSARDLSSRFNYTVASLHTCRLTVLQQQDPSP"
     gene            2622..3767
                     /gene="umuC"
                     /locus_tag="OHN28_004804"
                     /pseudo
     CDS             2622..3767
                     /gene="umuC"
                     /locus_tag="OHN28_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
BASE COUNT          957 a          947 c         1003 g          866 t
ORIGIN      
        1 ttcacctgtt ttagaatctt cctggagggg gtgggggtta cagacaagtt cccattgttc
       61 gatatattcc ccgataatag ggtcaactgg ctttgtgaat gctgcgtaag tcttgctaac
      121 gggtacatct atcagacaga tacgggatac attattttct tctggctgtt gccagcggat
      181 acacccacac tgcatacgta gaatttcatc acgtctgagg cctccaaata accagagaac
      241 gctgagtgct ctgaccatct ccagtggata aaaataagga tatgcaacac agtttggtaa
      301 atccgactcc tgcaggttct gtccggcgtg aagtaattta caccacatat catcagccac
      361 aatacgaggc tctggtccaa ccagggaagt caagctacgc ggtgccctga aaactctttc
      421 tgggctgaat cgtacaggta tccatcccca ttcctgcaag tcatgaaaga aagttctaat
      481 acgacccaac atcctagctc tcgctgcagg catcatcatt ttgccacgac ggtcacgata
      541 catgccccca gggtttgccc attcaccaac tttcattcta caagcggcgg tcacccaggc
      601 gatcgcggtg tcccgtgtcc aatcagatgg tgacagacag gttggatagc actcagctaa
      661 ccagctgacc acactttagc aaaacgtacc aaccggacaa aatagatgaa gggcgaagta
      721 ctgaggtagc acgccaccgt ttgcaccata ccagccattt agttggtaca tcggctgttg
      781 cattatattc gcggggagga cggcgacgtt gtgttattcg gtaatcaaac ccttcctcaa
      841 tagtccctga tgcagccagt atacgcgata gagtagctga ggcttcccgg aggtaatccg
      901 ggcacgttgt agcaatctgt tgcaacgtgg tcacggtgat atggtctagt tgagcatccc
      961 tttgcagaag cattgccata cacatggcat ttctgatgcc gttgttccga ctatcacgag
     1021 gcacatatcc cagaacctgc atcatattgt caagctggct gacggcaaaa tcggtgctct
     1081 gtcggccaaa aaccttaaga cagagccgat attgatagaa tatgccacag tgttccagcc
     1141 gctcaaaccc acatagcaac catgctaacg ctataacata ttgccgacag ttgcctctgg
     1201 ctccaaaccg tcgacgaaat tccccttcgc tgttgcaaat aacttctaac cactcttctt
     1261 gtgtccaggc ccagtatgtt ttccggatgc gctgcatctc caggactata tcgtgaatag
     1321 atgaattgta gggggccttg gccgcatgca tttttgttag cgtgtcgcgt agtggtaaaa
     1381 gaaggcggga aagccgtttt tgtatatcag gtgaccagtt tttacctcct tgctggaagt
     1441 ggtcgaccaa tgtatccagt gccgcaagct cgctttcaga gatctccggt cgggtatccc
     1501 aaagtagggc aggaagactg agtgatgttg tcggacaagg cgctgtatgt tcagattgca
     1561 tcatggagaa ggatcctgct gttgtagtac agtaaggcgg caagtatgca aggatgcgac
     1621 cgtgtaattg aaacgactac tcagatcacg tgcgctgaga tgaatataga gcagagttga
     1681 ctggatacga cggtggccag cgaatgcagc aatttcgtgg atgtcccacc ccacgcgtgc
     1741 cagttctgtc agacaaagat gtcggaatgt gtgggtgctg atgagtggaa gatcagcctt
     1801 aatagctaaa ccgcgaacta cttttgacca agtccagttg ctaataggtt ctgcccgatt
     1861 acgcggagag cgtgagagaa acaacggacc gcgagatgag cttagcatac gacgttcagt
     1921 gagccatgct gtgtagagtt cccccgtcac aggagaatag ggcacaaccc gaccaaagcg
     1981 gtttttggta tgctcagcgc gcacagtgag caagcgttga gaagggtcaa tatctccggt
     2041 agctactgtg caaagttctt ctctgcgtaa tccgcagtcg taggccagtg ccagcataaa
     2101 acgagtacgg atatcagctt gtccggctac ctcaagaaag cgcaaccact ccgcatcatc
     2161 cgggatccac ggtagccgcc gctgcaacgg cactatacca gcttttcctt tccctccgtt
     2221 tttccagaca cctcgtctga caggatttct ctcccgtatc ccctcctcac aaagccaatc
     2281 gttgtacagt cgtaccaccg tcagccggtg gcggatagtg gcagcggcca gctggcgacc
     2341 ggtatgcagg ctgcggacat agagtgcgac cgtatcacgt ccagcgacag cagccgtcag
     2401 ggcaacagag tgacagaaag cgagccagtc attgacgcca cgggcatagg catcaatggt
     2461 gtttggagca cacagcaggt cctcctgcag gcacacccac cggcaagctg aaggatcggc
     2521 cgacaggaaa ggcagcgcat ccagcagttt cttgtgcaca gcatccccct tttcgataac
     2581 agaatgtgtt gtatgaaata tagtcaattc caaataacat agaagcgaag cggctgggca
     2641 taaaaatggg agtaccgtgg tttcagctga agtcggtgaa atttccggag ccggtcatcg
     2701 ccttctccag taattacgcg ctatacgcat cgatgtcgaa cagggttatg tctcatctcg
     2761 aggagctggt gccgggcgtg gagcagtaca gtattgatga gatgtttctc gacgtgcggg
     2821 gtattggcag ctgcatcgat ttcgaggatt tcggccggca gctgcgtgaa cacgtcagga
     2881 acgggactgg cctgactatc ggtgtcggaa tggggccaac caaaacgcta gcaaagtcag
     2941 cacagtgggc ttccaaagag tggccatagt ttggtggcgt gctggcgtta acgctaggca
     3001 atatccgccg tacggaaaaa ctgctgtcac tgcagccggt ggaggaaatc tggggggtag
     3061 gccgcaggat ctcaaagaag ctgaacacaa tggggataac aactgcactg cagctggcgc
     3121 gcgcgacccc tgtatttatc aggaaaaact tcaatgtggt tctggagaga acggtccggg
     3181 aactcaccgg agaaagctgt atttcactgg aggaagcacc gccccccaaa cagcagatag
     3241 tctgcagcag gagctttggg gaacgtgtca caacttatga agccatgcga caggcagttt
     3301 gtcagtacgc tgagcgtgca gctgaaaagc tgcgcggcga gcgtcagttc tgcaggcata
     3361 ttgccgtgtt tgtgaaaaca tcgccgttcg ccgtaaatga gccctactac ggtaatatgg
     3421 ccagtgaaaa actgctcatc cccacgcagg atacacagga catcattgcc gctgccgtca
     3481 gagctctgga caggatctgg gtggatggtc accgttacgc aaaggcaggc tgtatgctga
     3541 acgatttcgc tcctgacggg gtttcgctgt tgaacctgtt cgatgatact ctgctccgaa
     3601 gcaacagcaa tcagctaatg aaagttgtgg acggtattaa tcattcagga ctcggaaaag
     3661 tgtggtttgc gggccggggg attgcatctg aatggcaaat gaaacgggag atgctttcac
     3721 cagcctatac aactcgctgg tctgaactac cagtagcgaa attatgagtc acg
//
LOCUS       DAJOTB010000273         3724 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJOTB010000273 DAJOTB010000000
VERSION     DAJOTB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.274"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..278
                     /locus_tag="OHN28_004805"
     CDS             <1..278
                     /locus_tag="OHN28_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCQ2300050.1"
                     /translation="AGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIG
                     AHTCIIGLNVKIGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            381..1046
                     /locus_tag="OHN28_004806"
     CDS             381..1046
                     /locus_tag="OHN28_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300051.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSKTVTPDSNLKDACIH
                     NGA"
     gene            1104..1394
                     /locus_tag="OHN28_004807"
     CDS             1104..1394
                     /locus_tag="OHN28_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300052.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGGA"
     gene            complement(2072..2548)
                     /locus_tag="OHN28_004808"
                     /pseudo
     CDS             complement(2072..2548)
                     /locus_tag="OHN28_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2543..3576)
                     /locus_tag="OHN28_004809"
                     /pseudo
     CDS             complement(2543..3576)
                     /locus_tag="OHN28_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1051 a          850 c          856 g          967 t
ORIGIN      
        1 atgcaggaat cgtcattcat ggtaatgttg atatcggtga gaatgtcatt atacggcaga
       61 atacaaccat tggtgaaaag gtttctgaca gtagggaaaa ctatattgtt attggtgata
      121 atgtcgatat tggtgcccat acatgtatta ttgggttaaa tgtgaaaatc ggaagtaatg
      181 ttaaaatcgg ggcaatgtct tttattatgg aagatgtacc tgataactgt acttatgtca
      241 ccaggaagga atccagagtg ataattcgcg aacagtaatg aaatacatgt tattattctt
      301 ctgttggtga ctttaccgac tcagatatat actatctgta tgtcgtgcat tatatattaa
      361 cgatacgcag aggtgtgtat atgggcagtg ttacaaagaa accgtcacct gttgttatta
      421 ttaagaaaaa aagacgaatt ctttctcatc aggatgatgc tgtacaggca gttcctgatg
      481 atataaaacc agcgccagta atggaattta aaaccgactc attacctcct gaaataccag
      541 ccacagtggc cggttccccg gaacaacaga aaaaaacagg acgtaagaag aagaaaaaaa
      601 agaagaacaa tagatggaat catgagttta tccgacacag tctggactgt gttaaaaaaa
      661 cattccctca gttacgggct gaagaggggg gatatcatgc atttaaaata ggcatctcaa
      721 aggatgtcat ggcttttatt tcggccagtc ctgagtgtgg actaactgta aaagaatgga
      781 attgtgctgt aaggatgatt actggcggtt acaaatatct gcagcgtata tcagtcgcag
      841 gtactccgcg ttatggtctg gatggcaaag ttcatggaac tgtaacggag gaagaagcgc
      901 tgtatgcgca ggcaaaactt gaaaaacaca cgcagcgtta taacgccagg atgaaagaga
      961 cagaagatgc tcgttgcaac caaccatcga aaactgtcac accggacagc aatttgaagg
     1021 acgcctgcat tcataacggg gcataattat gtgaaatgct gtcccgctat ggaatcaaaa
     1081 ttgagtaacg acacgaggtt ttgatgatta ctgttattat ttctgaggtg gatggctggc
     1141 gtgaatggaa acaccgcgcc cgaacaaagg atgcacagac agccatcatc cgggctatga
     1201 ataaacattt tccacgatct tacaatttta tccctgatga tattgataat gcacctgtat
     1261 tatttgcagc tgtaacacgt acaccgaacg tgaaaataac cggtcatatc tggaaaccta
     1321 tgtggaacag gggaatttgc tggaacgtca aaggtccgcc agtaatcatc acgctcatcc
     1381 aagggggcgc ttgaaattcg gaaaataaac cacgttaaga ctatttgggg ttctaagccg
     1441 aaaacctaga aaattccgta accaaagcca gtaattgaca gatttgcatg gcgttgaata
     1501 ggtgacgggt tatgtgacga aatctgatgc agaaatcgtt gtttcagtga cagtcactca
     1561 atcggtcgtt tatgtgacaa cccacgccgt tactggtcgc ggaaaaatcc aggtttcggg
     1621 gtttgaggct cagtggaaca ataaccgccg ttaagcggtc attttaaata gccaaaaatc
     1681 aaccagccaa tttacatcgt gaatctgcgt tcttggctat ctgttttggg gggggccaag
     1741 ttggctttac tcatcctggc cgtttgcgga acaaacgacg ttgttgatct caacagattg
     1801 atttacctgt tattgaccat tcaattgaac cgcttaaccg ccttttgagt tccactactc
     1861 cccaaacccc aaatagctgt ctagcgcgaa aaatcactgc ccgtaacgta tcatatggct
     1921 ggtacgaata aattctcact tactgcacca taaacgtaca cgtcaaaacc ttccaaacat
     1981 gcttcaaaaa tcaacaaatt cgctgtatcg gaaatttcgg cgtttccggc gtacaacccc
     2041 gattagatat aaatgaatta acgtgtgtgg tttattggtc tctgccttct ccagcaatga
     2101 cgcaagactt tgacatacca tcaaaaaaat gatcggcgag ctccagaatt cgtggattat
     2161 gagttgtaat aataacggta gggcaatgct gccggatata ggtaatgatc tttatcgcga
     2221 gttcttcgcc aatagaggcc gtgggttcat caagtacgat aatctttttc ttacgggcta
     2281 gggcgcgggc tatgcataat cgctggatct ctccaccact caaaccacta cccagaggat
     2341 taatatcccg gtctaggaaa gcaaggaaat gttctccgca gtcctcttta attaacccta
     2401 atgaattcag gatgcaaaac agttcatcat cgggaagaat gtcatcggtt ccatacagaa
     2461 tattatcacg aatagttccc tgcaccagaa cggctttttg aggcacaaca gcaacgtcct
     2521 ccaggataga cgcactcaac tctgtgttct tcggttcaag ccccaacatc cgggcaaact
     2581 gccgtgtttc attagcccgg tagcatgaac cattatccgt cagccactct actggagacg
     2641 ccggaagctc gttgccgaag cggcgttcca ccgctcctgg catgacgtcc tgtactgttt
     2701 cactgtcgaa gccgcccgta gtgaccgccc agtacagtgc ctcacggtca cagcagtcca
     2761 gcgcgaacgt gactcgcagt ttttctccgt tatcacagcg gaactcgaac ccgtcagagc
     2821 accatcgttg attgctttct ttcacggcca ctttgccagt atgtgcccgc ttcgatggcg
     2881 gtacagcggt ttttcgctca agcaacagcg cattctggcg catgatccgg taaacacgtt
     2941 tggcattgat cgcaggcata ccatcaagtt cggcctgtct gcgaagcagc gcccataccc
     3001 gacgataacc atacgtgggc agctctccga taacatggtg tatacggaga agcacatccg
     3061 tattatcgga gtgacggctg cggcgaccat ctttccagtc atcggttcgt ctgagaatga
     3121 cgtgcaactg cgcacgcgac acccggagac aacggctgac taagcttact ccccatcccc
     3181 gggcaataag ggcgcgtgcg ctatccactt ttttgcacgc ccatattcaa cggcttcttt
     3241 aaggagttca ttttccatcg tttttttgcc gagcaggcgc tggagttctt taatctgctt
     3301 catggcagca gcaagttcag aggcaggaac gacctgctct cctgcggcca cagcagtaag
     3361 acttccctcc tggtattgct tgcgccagag aaatagctgg ctggctgcca caccgtgttg
     3421 ccgggctaca agggagaccg tcattcccgg ttcaaaactc tgctgaacaa tagcgatctt
     3481 ttcctgtgta gtacgccgtc tgcgtttctc cggtcctaag acatcaatca tctgctctcc
     3541 aatgactagt ctaaaaacta gtattaagac tatcacttat ttaagtgata ttggttgtct
     3601 ggagattcag ggggccagtc tagcagatta tggtgcgcag ttatacaaca gcagtaatga
     3661 ggagtctact atttctcttg ttatgccaga tgcaattgaa tggaacggaa agaatacgcc
     3721 aaga
//
LOCUS       DAJOTB010000274         3620 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJOTB010000274 DAJOTB010000000
VERSION     DAJOTB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.275"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..518
                     /gene="emrR"
                     /locus_tag="OHN28_004810"
     CDS             <1..518
                     /gene="emrR"
                     /locus_tag="OHN28_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCQ2300053.1"
                     /translation="TPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENRNKMLK
                     AQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIERRESD
                     NDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLSRLDQM
                     EQGGVVLEAMS"
     gene            645..1817
                     /gene="emrA"
                     /locus_tag="OHN28_004811"
     CDS             645..1817
                     /gene="emrA"
                     /locus_tag="OHN28_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCQ2300054.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            1834..3372
                     /gene="emrB"
                     /locus_tag="OHN28_004812"
     CDS             1834..3372
                     /gene="emrB"
                     /locus_tag="OHN28_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCQ2300055.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
BASE COUNT          826 a          971 c          967 g          856 t
ORIGIN      
        1 ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc
       61 aggagatcct tctgacccgt ctttgcatgc atatgcaaag caagctgctg gaaaaccgca
      121 ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg
      181 agtctcagga aaaccacagt attcagcctt ctgaattgag ttgtgctctt ggatcatccc
      241 gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgtg
      301 aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt
      361 tgcgcgaggt tttaccgccg cagcataact gcctgcatca actctggtcc gcgctcagca
      421 caacagaaaa agaccagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga
      481 tggaacaggg cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc
      541 cagatttata aaagaaaaaa taaccggcca gcatcgcaac atgctggcct ttttggcaag
      601 caggtcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg
      661 agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc
      721 ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg tttttggtac
      781 tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt
      841 ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag
      901 gcgacgtgct ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa
      961 ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc
     1021 aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc
     1081 gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg
     1141 ccgtcaccag tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg
     1201 cgatgattct ggggacgaaa ctggaagatc agcctgccgt acaacaggct gccaccgaag
     1261 tacgtaacgc ctggctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg
     1321 tctcccgccg cgcggtacag cctggggcgc aaattagccc aacgacgccg ctgatggcgg
     1381 tcgttccagc caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata
     1441 tgcgtatcgg tcaaccggtc accatcacca ccgatattta cggcgatgat gtgaaataca
     1501 ccggtaaagt ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc
     1561 aaaacgccac cggtaactgg atcaaagtcg ttcagcgtct gcctgtgcgt attgaactgg
     1621 accagaaaca gctggagcaa tacccgctgc gtatcggttt atccacgctg gtgagtgtca
     1681 ataccactaa ccgtgacggt caggtactgg caaacaaagt acgttccaca ccggtcgcgg
     1741 taagcaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa
     1801 aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc
     1861 gcacaactgg tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctggac
     1921 tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc
     1981 cagggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc
     2041 ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tctggtccac catcgccttt
     2101 gctattgcgt cgtgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc
     2161 gtgattcagg ggattgtcgc cgggccgtta attccgcttt cgcaaagtct attgctgaat
     2221 aactacccgc cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtt
     2281 gcgccaattt gcggcccgat cctcggcggt tatatcagtg ataattacca ctggggctgg
     2341 atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaactctg
     2401 cgcggacgtg aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg
     2461 gttattggta tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactggttc
     2521 tcatcacagg aaattatcat cctcaccgtg gtggcggtgg tggctatctg cttcctgatt
     2581 gtctgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac
     2641 ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgcgattgtt
     2701 ctgctgccgc agttgttgca ggaggtctac ggttacacgg cgacgtgggc agggctggcc
     2761 tctgcgccgg tagggattat tccggtgatc ctgtcgccga ttatcggccg cttcgcgcat
     2821 aaactggata tgcgccgact ggtaaccttc agctttatta tgtatgccgt ctgcttctac
     2881 tggcgtgcct atacctttga accgggtatg gattttggcg cgtcggcctg gccacagttt
     2941 atccagggtt ttgcggtggc ctgcttcttt atgccgctga ccaccattac gctgtccggt
     3001 ttgccaccgg aacgactggc ggcggcatcg agcctctcca actttacgcg aacgctggcg
     3061 gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc gctgcaccat
     3121 gcacagttga ctgagtcggt aaacccgttc aacccgaatg ctcaggcgat gtacagtcaa
     3181 ctggaagggc ttggtatgac gcaacagcag gcatcaggct ggattgccca gcagataacc
     3241 aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc
     3301 gtcctgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc
     3361 ggtgcgcact aaggttgcca aagggagctg cggctccctt tttatttgct cattctgcaa
     3421 acggttgttt tatacactca ttcggtgtat cttttcgccg ccccaaactc ttccattcac
     3481 cattagccgg ttctgagcat tcgctcaggt tgttaattct ctttttcctt cgccctcgct
     3541 ttcgcgggtg atactcacat ttttctttta accccaaaac tcgcaccctc gcgggcgtac
     3601 atttattaca cacaaaataa
//
LOCUS       DAJOTB010000275         3524 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJOTB010000275 DAJOTB010000000
VERSION     DAJOTB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.277"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            35..895
                     /gene="aac(3)-IId"
                     /locus_tag="OHN28_004813"
     CDS             35..895
                     /gene="aac(3)-IId"
                     /locus_tag="OHN28_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HCQ2300056.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            908..1450
                     /locus_tag="OHN28_004814"
     CDS             908..1450
                     /locus_tag="OHN28_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2300057.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQL
                     LHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDS
                     GAHTSDELAAMVLDGIDGRT"
     gene            complement(1932..2123)
                     /locus_tag="OHN28_004815"
     CDS             complement(1932..2123)
                     /locus_tag="OHN28_004815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300058.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     gene            2147..2374
                     /locus_tag="OHN28_004816"
     CDS             2147..2374
                     /locus_tag="OHN28_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300059.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     gene            2425..>3524
                     /locus_tag="OHN28_004817"
     CDS             2425..>3524
                     /locus_tag="OHN28_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HCQ2300060.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRD
                     PKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRIIRIGEDWS
                     EEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQNISAVLDA
                     LGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVE
                     PPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPEL
                     FQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAG
                     DQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPT"
BASE COUNT          713 a         1016 c         1042 g          753 t
ORIGIN      
        1 gtatagtgtt ttgcagttta gaggagatat cgcgatgcat acgcggaagg caataacgga
       61 ggcgcttcaa aaactcggag tccaaaccgg tgacctcttg atggtgcatg cctcacttaa
      121 agcgattggt ccggtcgaag gaggagcgga gacggtcgtt gccgcgttac gctccgcggt
      181 tgggccgact ggcactgtga tgggatacgc gtcgtgggac cgatcaccct acgaggagac
      241 tctgaatggc gctcggctgg atgacgaagc ccgccgtacc tggctgccgt tcgatcccgc
      301 aacagccggg acttaccgtg ggttcggcct gctgaatcaa tttctggttc aagcccccgg
      361 cgcgcggcgc agcgcgcacc ccgatgcatc gatggtcgcg gttggtccgc tggctgaaac
      421 gctgacggag cctcacgaac tcggtcacgc cttgggggaa ggatcgcccg tcgagcggtt
      481 cgttcgcctt ggcgggaagg ccctgctgtt gggtgcgccg ctaaactccg ttaccgcatt
      541 gcactacgcc gaggcggttg ccgatatccc caacaaacgg tgggtgacgt atgagatgcc
      601 gatgcttgga agagacggtg aagtcgcctg gaaaacggca tcggattacg attcaaacgg
      661 cattctcgat tgctttgcta tcgaaggaaa gccggatgcg gttgaaacta tagcaaatgc
      721 ttacgtgaag ctcggtcgcc atcgagaagg tgtcgtgggc tttgctcagt gctacctgtt
      781 cgacgcgcag gacatcgtga cgttcggcgt cacctatctt gagaagcatt tcggaaccac
      841 tccgatcgtg cctccgcacg aggccgtcga gcgctcttgc gagccttcag gttagaggcc
      901 gtcgacaatg ataatctgga tcaacggacc tttcggcgcc ggaaagacga cgctcgctaa
      961 gcggctgcgc gatcggcgtt ccaaatcgct gatctttgac cccgaggaaa tcgggttcgt
     1021 ggtgaaagaa acggtcccca tgccagcgag cggagactat caggatctcc ccttgtggag
     1081 gggacttacg atcgcggcgg tcagggagat tcgaaggaat tactcgcagg acatcatcat
     1141 cccaatgacg ctcgtgcacc cggactatct gactgagata ctcgacgggg taaggcggat
     1201 cgacgatcag ctgctgcaca tctttctgac gctcaacgag gacctattgc gtcaccggat
     1261 cgcgaaccag accatgcatc ctgacccgaa tcgaaatgcg gagattcgag agtggcgatt
     1321 agcgaatgtc gcccgatgct tggccgcaag ggaacggctt ccatgcacaa cccgtgttct
     1381 cgatagtggt gcacacacca gcgatgaact cgcagcgatg gtgctcgacg gaatcgatgg
     1441 gcgcacctga tcgccttcga cgcctgcgca aagcgtagcg cgagggtggc gggctcacga
     1501 ccaaacgccc agaggtcgat catcgcaggg atgtttggct ttgtggtgcg gacgacggga
     1561 ctcgaacccg tactctcaca gagaagcaga ttttcgtacc acctcgactt tcgccgccgt
     1621 ctgatgacgt tcgtggtctg gactgtccct tcgccattgc ccgaaggctt taggcgccgc
     1681 ccgtccagtc tctacacctt cccccgaagg ggcttggctc gggattggct tagggtattg
     1741 cccgttagcg ttccccgact ttgagcggtt ctactccgcg gatttccccg cgggcactcc
     1801 aattttaaag tctgctgcgt ctaccgattt cgccacgtcc gccttttttc gccgttccta
     1861 gcgctcgtgc gatgcaccta tgttgcacct agcgccgaat cgttcttcgt catcctgaaa
     1921 aaccacgtct cctaaagcct tgcatagctt atcttttctc caccacgaac ttttttgtgg
     1981 gatggtagaa aaaaagactt tttaagtccg ctggcttgcc aggccttgtt agcttgtacg
     2041 gtcatggtta tcgggtaaag aatattgacg gcatcgctgg tgtcggtggc tgaaaagccg
     2101 gctcccatca gggcaatagc catttcagat gcaggcgtac agggcaatgg tcaacagcta
     2161 cagcctgtct gacgattccg gcgtcatggc tgcggcggct atcacgcatt ttttgttcgg
     2221 tcaggcggtg ttttcgtacc tcaatggttg gagcgtgttg atcggacctg gtacaggttt
     2281 ggacagcacg ggctgcaaat acgcaaggga tttaatgggc ctggtggcgt tcacggcttt
     2341 tatcgtgacg tttctgttca ggggctactc ataatctcgt ggctcggcgg ttcccggcac
     2401 accatgacag taaggaagga ccctgtgtct caactctccc agcttcgaag ccccgccgcc
     2461 gtgcaggctg ccatcgatga gttcgtgcaa ctgggccgca cgaaattcct ggcgcgccac
     2521 ggctacggca agtcccgcga cttcctggta cgtgatccga agaccggcac cgattgcgat
     2581 tccaaggcca tcgccggtgt ggccttcggc aagcaatttc ccgagcaggg cccgctcact
     2641 gctgacagct tctccggtgg cgaggcgacc gtcgttccgg cgctgacgcg gctcgggttt
     2701 cgcatcattc gcatcggcga agactggtcc gaagaagagg tcctggccac ggtcgaagac
     2761 tatttcgaca tgctgcgtgc cgaggcggct ggggagccgt acaacaagtc cgagcacaac
     2821 caggcactgc gccaactgct gaacggtcgc agcaagtctt cagtcgagct caagcaccag
     2881 aacattagcg ccgtactcga tgccctgggc ctgccctata tcaacggcta caagccacgc
     2941 ggcaacagcc aactgctgct gcgtaaatcc gtacacgcct acgttctgga acatcagcag
     3001 acggtcggcg ctcttgtcga tgccctggag gaggtaaaac ttccgggtga caaaacctac
     3061 cgagcggctt tggtagaacc acccgcccgt gaagtgcttg tgcgtacccc ggcatctcta
     3121 cggcaacgcc taccgcgaaa gttcgattat gccgctcgcg atgaagccaa ccgcaagctg
     3181 ggccgggcag gggagcagtg ggtgattggc tacgaacagc aacgcctgac cgagctcggc
     3241 cacccagagc tttttcagcg gctggattgg gtgtccgaca cccagggaga cggtgcgggg
     3301 ttcgacatcc tgtcgttcga agaggacgcc catgagcgct tcatcgaggt gaaaaccacc
     3361 aatggcgggg taggctcgtc tttcttggtc agccacaacg aactcgaatt ctccaaggag
     3421 gcgggcgatc aattccatct gtatcgcgtg ttccagtttc gggacggtcc gcgcctgttc
     3481 acgctacccg gcgacctcag ccaacatgtg catctcaagc cgac
//
LOCUS       DAJOTB010000276         3488 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJOTB010000276 DAJOTB010000000
VERSION     DAJOTB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.278"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..373)
                     /locus_tag="OHN28_004818"
     CDS             complement(<1..373)
                     /locus_tag="OHN28_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-kinase"
                     /protein_id="HCQ2300061.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALL"
     gene            complement(373..1176)
                     /locus_tag="OHN28_004819"
                     /note="aph(3'')-Ib"
     CDS             complement(373..1176)
                     /locus_tag="OHN28_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCQ2300062.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1576..3012)
                     /locus_tag="OHN28_004820"
     CDS             complement(1576..3012)
                     /locus_tag="OHN28_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCQ2300063.1"
                     /translation="MSTLDVQKLKDDAVEWALTHGVAFKESSYSAVHTPFTLTPTPIS
                     RKSYQYLKNATGILSKLIYSVSEDHDFLYSAIYPIKAGNAFFSALLNMHQQIHSSSRH
                     APRLPLLIMRSDFMDDNRDGFRLIEFNGIAAGMGPFGQRIHDLHHYLQRQCPAVFSDL
                     SNQATGALVDNQAIEGLSAAICQATCRIKQEFGDPGPARFLMIVQENENNVFDQHLLE
                     LALQQKGIETHRRTFRELHNQLSTGADNRLILEGVGPLDTVYLRAGYQYSDYEAFNID
                     EQECCQALMATRVFIEQHRVAVNATVGQQLATSKRVQMLLSQLGYDEFVAFGLTLQEA
                     KIAKTILGEMLPISPDSINLAKESPSETWVLKNQGEGGGHCLFGADILTKLTELTPQQ
                     YQSWSLMRRLHPQPRAMPTLIVRKGELHKVNDLISELGMFSVQTDNNPSSAEHSFAGY
                     LIRSKSAESTEGGVHSGQGVLDSLVYSD"
     gene            complement(3180..>3488)
                     /locus_tag="OHN28_004821"
     CDS             complement(3180..>3488)
                     /locus_tag="OHN28_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCQ2300064.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT          884 a          900 c          830 g          874 t
ORIGIN      
        1 ggagaagggc agaaggcagg ggttcctcag atgcggcata cagcttcgcc attagttccg
       61 ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag agcattcgct
      121 ccccggcata ttcgagcaac atcagattgt tctcacgacc gagcaaccgg actgctcccc
      181 tcccattgcg ccataccaga tagtcggccc cgcgcagttc atcagcaatg tcttctatag
      241 gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc caaacgaggc
      301 tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca ggaaaaacag
      361 gcggcatgaa catcaacccc aagtcagagg gtccaatcgc agatagaagg caaggcgttc
      421 gcggtcgggg gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc gctctgcttc
      481 atctggcgct gcccagttct cttcggcgtt agcaatcatg agtgccaaat cggcatagcg
      541 atctgctgtt ccgagccgcc caaggtcgat cagacccgtg cattgaagag ttttagggtc
      601 caccatgaag ttcggcatgc agggatcacc atggcaaaca accatatcgg tgcgctcttg
      661 gtcgagccgc accggtagct ctcgttcgac acgagccaaa agatcgagct gcggcgtact
      721 cttgtcctcg tccggtaaga agtcgggatt gacggcattg cgggacacca catcaacggc
      781 gcgtccgaac attcgcgaca gcctgcgctc aaacggacat tgatcaaccg ataggctgtg
      841 aacagcgcca agttgctgcc ccattgacgg ccacgctttg agcaaatccg ctccagacag
      901 atcagccgcc ggtactcccg gaattgccgt tatcaccaag catgcaccct cctgttcctc
      961 ctgccagttg atcacctcgg ggcaagccac acctcgacct ttgagccaaa tgaggcggtc
     1021 acgctctcca gcgagctcac cgcggcggga agcaggtgcg attttcgcga aggcatgccc
     1081 gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca accagtcaga
     1141 atgcgattca ccaaaaaaaa tattagttcg attcaatgga ggttccttca gttttctgat
     1201 gaaggggtcc gcttggaaaa cggaaaatat cccacgctaa gactgtttgc tgtacaaatt
     1261 gatgtcgaca gcgcgtaatg accgtagtca caggaacagc taatggtatt gaaaattgac
     1321 ccgtaactga aacagcatcg acccagcagc gatgatgtga accttgaaga actttgggtg
     1381 cagcctctta tagcagcagt ttttgctgtt gttcccttaa taagatatgc cctgtaaata
     1441 ccctcatggc ttcatttata atactattat tagcttacta cgactcagtc tagaaacagc
     1501 atcatctcca ctggggctaa aacaccactt accgcatgct ttcatataac cagatttcag
     1561 cccccttgaa aaggactaat cgctgtatac cagcgagtct aaaacacctt gtccactatg
     1621 gacccctccc tctgtcgatt cagctgattt gctacgaatt agatatccag caaaactgtg
     1681 ctcagcactg gagggattgt tatctgtctg cacggagaac attccaagtt cgcttatcaa
     1741 atcattcacc ttatgcaact cccctttacg cacaatcaag gtgggcatag ctcggggctg
     1801 agggtgtaaa cggcgcatca gcgaccagga ctgatattgt tgtggagtga gttccgtcag
     1861 ttttgtcagg atatccgcac caaacagaca atgccccccg ccttcaccct gatttttgag
     1921 aacccaggtt tccgaaggag actcttttgc aaggttgata ctatccgggc tgatgggtaa
     1981 catctcaccc agtatcgttt ttgctatttt agcttcctgc agtgttaatc caaatgcgac
     2041 aaactcgtcg taacccaatt gcgagagcag catttgtact cgcttgctgg tcgccagttg
     2101 ctggccaact gtggcgttca ctgcaacccg gtgctgctca atgaatactc gggtcgccat
     2161 cagcgcctga caacactcct gctcatcgat attgaatgct tcataatcag aatactgata
     2221 tccggcacgt aagtagacgg tatcaagtgg gcctacaccc tcgagaatga gcctgttgtc
     2281 ggcgcctgta ctcaattgat tatgcagttc acgaaaagtc ctgcgatgcg tttcgattcc
     2341 tttttgctgg agggcgagct caagcaagtg ttgatcaaac acattatttt cattctcctg
     2401 cacaatcatc aggaacctgg cagggccggg gtctccgaac tcctgcttaa ttctgcaagt
     2461 tgcctgacaa atcgctgctg ataacccttc gatcgcttga ttgtcgacaa gcgcccctgt
     2521 tgcctgattc gataaatcag aaaagacagc gggacattgc cgttgtagat agtgatgcag
     2581 atcatgtatc cgctgaccaa acggccccat tcctgcagct ataccattga actctataag
     2641 cctgaaccca tcacgattat catccataaa gtcgctacgc ataatgagta atggtaatcg
     2701 aggagcatgc cttgatgatg aatggatttg ttgatgcatg ttcaggaggg ctgaaaagaa
     2761 ggcattcccg gccttgatgg gataaatcgc agagtacaaa aaatcatgat cctcagaaac
     2821 tgaatagatt aatttgctga gtattcctgt tgcattcttt aagtactgat aggatttacg
     2881 gctgataggt gttggtgtca gggtaaacgg ggtatgcact gcggaatacg acgattcctt
     2941 gaaagcaaca ccatgagtta aggcccattc gacggcatca tccttgagct tctgaacatc
     3001 aagagtactc attttacgtc ccctgatttt acctggcata tcattggtgg ttgttacttg
     3061 gctatcagcc tataaaattt gattgattac ttcaatagtt cttgtagcat tgaattatga
     3121 aaaaagaggc actgttgcaa agttagcgat gaggcagcct tttgtcttat tcaaaggcct
     3181 tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc ataataaaat
     3241 gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa
     3301 gccgtcttca tggatttaaa tcccagcgtg gcgttgatta tccgtttcag tttgccatga
     3361 tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc agacgggcac
     3421 cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg
     3481 atgaatcg
//
LOCUS       DAJOTB010000277         3431 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJOTB010000277 DAJOTB010000000
VERSION     DAJOTB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.279"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            266..1393
                     /locus_tag="OHN28_004822"
     CDS             266..1393
                     /locus_tag="OHN28_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300065.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFDFSIFHAKQPIEILAEHSKTSFH
                     LMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISADIKTNVRDLVDFQSSISHVK
                     LFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDDGLYHYTSDLTGTPLLRLKNT
                     VKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINWCNP"
     gene            1390..1905
                     /locus_tag="OHN28_004823"
     CDS             1390..1905
                     /locus_tag="OHN28_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300066.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            1907..>3431
                     /locus_tag="OHN28_004824"
     CDS             1907..>3431
                     /locus_tag="OHN28_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300067.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRYKSKESLDG
                     MKNQIKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQKDIEILLEKSS
                     QPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVDGQPRQSHGKG
                     "
BASE COUNT         1026 a          677 c          793 g          935 t
ORIGIN      
        1 tctacaccca tcaaagtgat ggttatgaaa agagataaaa atgctgatgt caaaagaggc
       61 ttatgctcgg cacagtggag tgagctgcca aactgtcgat gactgggtag ccggtggggc
      121 ggaagtagtt atgtcccgta gcaaggttaa gatttgctct tgtgtgtggg gaaccttagt
      181 caattacttt cctggcgcac tgtgttagat tttgtaaaat tttaaaagac taaagattta
      241 atatcacttc tccatggagg ttgtggtggt agagaagatg aaacaaaatt caagttcgac
      301 gcttggcgcg ctttctgctc ctacaacgtg gccgggtgcg aacgctcggc tgcttgggtt
      361 aggtgtaggg cttccaatag cccccttgga tcgtctggca cagtttagcg ccgccgaatt
      421 tgaaaggttt acactcgaat gggcctctga atacttagct ggtcagcaat atgtcaacga
      481 agtacaacaa cgtggcgggg caggagataa aggcagagat attgttgtat ggcttgatcc
      541 atctgatgct gttcctcgaa gatggcactt atatcaatgc aaacattatg atgcgaattt
      601 aggccttcct aaggcaggca tcgaagttgc aaaagttatc tattatactt tcatcaagga
      661 ctacacagta cctacacaat accattttgt tacacataaa ggggtaacat cgccattcca
      721 agacctccta gatgatccaa ctaaactcaa agagaaaatg ctatccgagt gggcaacttt
      781 ttcaaagcaa attacatcga aacatagtgt tgacctgacg cctgaacttg aaaaatatat
      841 taaagagttt gattttagta tttttcatgc aaaacagcct attgagatac ttgccgaaca
      901 cagtaaaacc tcttttcatc ttatggtctt tggcgcacca ttaattgagc gggatccacc
      961 aactaggccc ccatcatctg tggctcccat tgaaactgta tatattgaac aactcttttc
     1021 agttatcagc gctgatatca aaaccaacgt gcgagattta gttgattttc aatcaagtat
     1081 cagccatgta aaactctttg aacggtcccg aataactttt tattgctcgg aagggctaaa
     1141 ggaactcgct agggaccaaa tggcgaatca agaattcttt aattctttac ttgtagagtt
     1201 tgatgatggt ctgtatcact acacctctga cctgacaggg actccactcc ttcggttaaa
     1261 aaataccgtc aaggccgctc agaccctgca acttggtgcg cacccacttg ccattcacgt
     1321 aacgaataag gatcgtgaag gtatatgcca ccaactagcc aatacaaact tgattaattg
     1381 gtgcaatcca tgagcgaaat gcaagtattt gaaggaaact ttgagcagag accttttaac
     1441 actccgctgg agtatggatt tcgtgctctg ttcatattgg atgcagcgga caatgtagca
     1501 atggacctac aaaggatagt ttcgtatgac tatcttcttg tgcataccag tgacgtacct
     1561 agggggccta aaagccttca tccagcagtt cctcaccggg gagcagagct tcttgttaag
     1621 cgaagcgcaa ttcaggcagg gctgcattta atgctatcaa gagaattgat taaggttgtc
     1681 tttgctgccg aaggtatttt gtatcaggct accaatttaa caggaaggtt tgtgcggctt
     1741 ttgatgtcac agtattcaaa agagctcgca gaacgtgcct cttgggtcac aaagcaattc
     1801 tatgaatata cggatgaaga acttgcttca tatatatctc agaatgtggg gcaatggggt
     1861 tctgaattcg accgattgac cgcaattgat ctactggatt tataacatgc ttcgtataag
     1921 aaaaatacta ttgcgcggaa acagcgttca agacgcttat gttgatttct ataagggtgc
     1981 gaatattcta gcaggggaat ctgatactgg gaaaagttac cttgtgagtt gtcttgacta
     2041 cattcttggt gccgaaaaac tcaaaaaaaa gcttaaagag gccaccgatt acacccatct
     2101 ttatgttgaa ttcgaaaatg atgaaggggg ggtgttaacg ctcaagcgcg gtcttgaggg
     2161 ggggaaatta gaggcacatg atgtcgctat tcaagacatc catggagaag gtaaaataat
     2221 tgctcctgtg cgtaaaggaa cgagtaaggg gccagatgtg acgtccatac ttttcccttt
     2281 tgcggggatc aaagaggcga agcttcgcaa gaatgctcgt ggcgaaacgc aacgtttctc
     2341 gattcgaacg ctcgctccta tatttttggt agatgaagta tccattatcg acgaatactc
     2401 gccagtaacg ggacgttccg gttatgacga cactgctcga aaacgcatgt tttcatacat
     2461 ccttaccggg cacgatgatg gtggtgttac cgtagaggaa aagcctgaaa ttgtaaaagc
     2521 gaggttgatg gcgaagcttg aattcatcca ggatttgatt cgaccacttg atgaacgttt
     2581 ctcaatttgt tcgcccaagt ttccattaac gagctcagca gatgatttat cagatcaatt
     2641 gattgcacaa gcaattgatg aagttgagag ggctgcagcc gctatttctg acttgctcga
     2701 gggaataaaa atggagacag cgttaacgtt aaaaatagaa tctcagttaa tgggagtatc
     2761 tgaaattcaa tcgagatata gcctattaga agaacgttac cattccgacc tgaagcggtt
     2821 ggattttatt tccgaaggtt ctcactattt cacttcactt caggaagttc cttgttccct
     2881 ttgtggacaa aatttactac atccacacag tgagaacgct aaaaaattaa tgaacagtaa
     2941 tgaagtccga cgttcttctt tagctgaagc tgcaaaaatt catggatacc ttgcaggcct
     3001 gcaaaaagcg atgagtgatc ttgaccgaag aaaagaagct ctgaatatag acagatataa
     3061 gtcaaaagaa agcttggatg gaatgaagaa ccagatcaaa tatacttttg agcctcttct
     3121 tacactttcc aaagaaagcc tagatttagc tattgagcag cgagctgaaa gagactctga
     3181 gttgaatgat cgacagagat ggtttgagtt actaaaacat caaaaggata tagaaatact
     3241 gctggaaaag agcagtcaac cacggctcga atgggaggga ttatctaccc gtacgctggc
     3301 cgaactttgt agagaaattg aaactgtttt aaaagactgg aaatggggtg ccgagcctga
     3361 cgtttcattt aatgaaaaag agtatgacat cattgttgat ggacaacctc ggcagtctca
     3421 cggtaaaggt g
//
LOCUS       DAJOTB010000278         3415 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJOTB010000278 DAJOTB010000000
VERSION     DAJOTB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.280"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..482)
                     /locus_tag="OHN28_004825"
     CDS             complement(<1..482)
                     /locus_tag="OHN28_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2300068.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     "
     gene            complement(479..1507)
                     /locus_tag="OHN28_004826"
     CDS             complement(479..1507)
                     /locus_tag="OHN28_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2300069.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVTSKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1604..2419
                     /locus_tag="OHN28_004827"
     CDS             1604..2419
                     /locus_tag="OHN28_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2300070.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2808..2951
                     /locus_tag="OHN28_004828"
                     /pseudo
     CDS             2808..2951
                     /locus_tag="OHN28_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            2962..>3415
                     /gene="ogt"
                     /locus_tag="OHN28_004829"
     CDS             2962..>3415
                     /gene="ogt"
                     /locus_tag="OHN28_004829"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCQ2300071.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTG"
BASE COUNT          921 a          833 c          758 g          903 t
ORIGIN      
        1 cagtcgaggt tatcagtgat ccagcccccc agagttggcc ctagcgtcgg tgacagtgag
       61 gccagcgcac tgactaccgc agcagccaac cccaactctt taccctggta ataaataaac
      121 gccgtcgtga aaactaaagg gatcatggat gcgccagcag ctccctgtaa agcacgaaaa
      181 aaaatcatga tctgaatatt ccaagcaagt ccacacgcga tactcattaa agtaaatatt
      241 cccgcggata gagtaaaaag ccaacgagtg gaaaataccc gtgacagcca cccagagagc
      301 ggaataacaa taatctcagc aattaaatag ctggtctgaa gccaggccat ttcgtcttta
      361 cctgcggaca gaccaccacc tatttcattt agggatgacg atacaatttg aatatcaagc
      421 tgagcaataa ataatcccat acacatactg gcgaaagcaa tatgtttacg catagacatc
      481 atctctcatc aacactgacg gtgacggaca gtcccgggcg aagaagttgc tttaattcct
      541 ttgcgtcgtc aaatgcaatc cgtaccggga cacgctgaac aattttggtg aaattgccag
      601 tggcattttc tatcggaatc aagctgaaac tggctcctgt cgctggggaa aggctttcaa
      661 tgtgaccgtg aaacacttcc cctttgagaa tatcagcacg gatctcagcc ttcattcctg
      721 gcttcattcc cgctatttgg ttttctttat aatttgcatc aacccacaac tcactgactg
      781 gtactagtga aactaacgat gtgccgcctt ccacccaact gcctgtatgg gcgctgcggt
      841 ttgcgacaat gccatcaatc ggcgcacgaa caagggtata ttccagatcc agtagtgcct
      901 gagtcaaagc ggcctgaact tcttcgatac ttgcacgtac gttttcttcc tgagcagata
      961 aaactgccag ccgttgcttc tccaccagga aatcgttcgc ggctttgcgc tcctttgctg
     1021 cgacctgctg gtagtcaaac ctggcgttat caataatttg ttgcgatatt gccgctgatt
     1081 gtgcgagttt ttcataacgt tcagtatctc gcaggcgctt ttgctcctca tgttttactg
     1141 cctgccatgt ttcactggca ctttgaatta tcgactgctg catcgcaata gtggcttgaa
     1201 tgctttcgag attggctttg ctacttttta tttttgcctc aagcctggcg acatttgcac
     1261 gataatcccg atcatcgaga cgcaggataa tatcgccttt ttttacactc tgattatcgc
     1321 gcacctctat agcagaaata taaccagaaa ctttacttgt caccgtggtg atattaccac
     1381 caatgtaagc atcatccgtc gtctgtataa atcttccttt gcttacccac cagataaaaa
     1441 agacaacgcc agccaaaaga ataccgatcg ctaccacccc aataagctgt tttttagaaa
     1501 ttatcatcac tctttacacc gtatatatcc ggaaacttgc atacgtcata tttaaaattt
     1561 aacatggctt atttgatgat ttctgacaaa atacctactg taaatgacaa tcacactgca
     1621 acccaaagca tcaccagggc atcacattat cggactggat tcggaacatc tgaatggagg
     1681 tacagttccc tggcataaac atctctatgc ccagttgctt tacccggctg aaggagtcgt
     1741 tcgtgtatgg gctggagaaa gcgtctggct agtgcatgcc agcagcgcgc tttggctgcc
     1801 cccacaaatg ccgcataagt ttgttgcgac aggcaacgta cttttaaaaa ccgtactggt
     1861 ttcagaagcg gaaagtgaaa cgctgggaaa agtttgcttc atgacgggta tttcacccct
     1921 gttaagggaa ttactaattg caattaacca acttccaccg tcgcaaagta ccacagataa
     1981 acaacagtta cgtttttcag ccctcgagac actgatactt caggaaataa aaatgggggt
     2041 gaaaatgtca cttgaactcc cttggccaaa tgatgagcgg ctacagcagc tttgtgagaa
     2101 tttgctaaac aaccaagggt atctgccaac actggataat ctagcggata aaattaacgt
     2161 cagtagccgt acactcatgc gcttatttgt taaagagacg ggattaacat ttcgccactg
     2221 ggttcagcaa atgcacgtta tttctgcagt gacgcttctt gatgatggat attcgttgac
     2281 caaaattgcc catcgtctgg gctatgcctc agctgaatcg ttcggcaata tgtttaagcg
     2341 tcgaacgggc tactcgcccg gtaaatttac tcgtcgatta acgatgcatg attatgcaat
     2401 cacaagacaa atgatttaac ttatattttc gtgaaatctg tcactgaaga aaattggcaa
     2461 cggatgggta aaaccgttat aacactgtca ccggtcacca ggaccccagg ccggatttaa
     2521 gacgaggatg cactgctgtg tgtactgtag agtctggcgg atgtcgacag gctctatttt
     2581 tttattcatt ttttagccct gtaaataacc acattctcgc cgggattggg gaatgagtga
     2641 cgagatcagt ggtgtctggc ggctatcacg ccacagatca aataacagat aacctacggt
     2701 aaaatttcgc tgactggaat tatcccaccc tcgatccatg tgatggcttg ccctcatttc
     2761 ttcaggcaga aaaatcaaaa aatcgcttaa tactatcaga ctgcgacaaa ccagacgcga
     2821 aactgggtac ttactattcg ttagtcttac cctatccact tatctttttg gtggtatggc
     2881 tgctgatgtt gctggcgtgg tatcttgttg gcttgccgat aggtccgggt atttacccac
     2941 gtttgtctta agagagaacg gatgctgaga ttacttgaag aaaaaattgc cacgccactg
     3001 ggtccactgt gggtgatttg cgatgagcaa tttcgcctgc gggcggttga atgggaagag
     3061 tacagcgaac gcatggtgca gctgctggac atccattatc gcaaagaagg ctatgagcgc
     3121 atttctgcca ccaacccagg tggtttaagc gacaagctgc gtgaatattt tgccggtaat
     3181 cttagcatta ttgatacgct tcccactgcc acggggggga cgccatttca gcgcgaagtc
     3241 tggaaaacac tgcgcactat cccctgcggg caggtaatgc attatggcca actggctgaa
     3301 caattaggcc gccctggcgc ggcgcgtgcc gttggtgcgg caaacggatc gaatcccatc
     3361 agcatcgtcg taccttgcca tcgggttatt ggccgaaacg gcaccatgac cggat
//
LOCUS       DAJOTB010000279         3347 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJOTB010000279 DAJOTB010000000
VERSION     DAJOTB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.281"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            125..841
                     /gene="nanC"
                     /locus_tag="OHN28_004830"
     CDS             125..841
                     /gene="nanC"
                     /locus_tag="OHN28_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCQ2300072.1"
                     /translation="MKKAKILSGALLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            861..1967
                     /gene="nanM"
                     /locus_tag="OHN28_004831"
     CDS             861..1967
                     /gene="nanM"
                     /locus_tag="OHN28_004831"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCQ2300073.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGVIDNDTVYIG
                     LGSAGTAWYKLDTKAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAENYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVAVQN"
     gene            2032..3012
                     /locus_tag="OHN28_004832"
     CDS             2032..3012
                     /locus_tag="OHN28_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCQ2300074.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMLGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
BASE COUNT          977 a          724 c          788 g          858 t
ORIGIN      
        1 ataaaataca gctactcgtt gccaaagaca ctataagcct ggcaaaaaat attacacaac
       61 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
      121 atatatgaaa aaggctaaaa tactttctgg cgcattatta ctgtgctttt cgtcaccact
      181 gatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
      241 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gtatggaaag
      301 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
      361 ggtggaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
      421 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
      481 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
      541 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
      601 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga
      661 ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca
      721 tatgacgccc gatattacgc catacataga atatgactac ctggaccgtc agggtgttta
      781 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
      841 gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc ctaatggctt
      901 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
      961 gagtaattga taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca
     1021 agctggatac aaaggccaaa gataaaagat ggacagcgtt agctgcattc cctggcggac
     1081 cgagagatca agcaacctcg gcatttattg atggcaatct gtatgtgttt ggcggcattg
     1141 gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     1201 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggc catgtgactt
     1261 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     1321 attttgaaga tctcaacgaa gctggaaaag attcaaccac tatagataaa atcaacgccc
     1381 actattttga caaaaaagca gaaaattatt tcttcaataa gtttctgttg tcttttgatc
     1441 cctctacaca gcaatggagt tacgctggcg aatctccctg gtacggaacg gctggtgcgg
     1501 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     1561 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     1621 ctcccgtcgc atcaccagat ggcgtcgctg gcggttttgc aggcatgagc aatgactcac
     1681 ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     1741 actatgcgca tgaaggcctg aaaaaatcat atagcgctga tattcatctt tggcataacg
     1801 ggaaatggga taaatctggc gaattatcgc aaggtcgggc ctacggagta tcattgccct
     1861 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagca gtgactgatt
     1921 cagttttgat ttctgtgaag gataataaag tcgcagtaca aaactaacgc ttcagggccc
     1981 cggtaaaggg gccctgctac caatttgtca tttcaattac gcgaaaatta tatggacgca
     2041 ataatatcgc ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     2101 tatggcgaag gactgccatt accggacggg gaagatgcgc ctcatcccag aattaaacaa
     2161 ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa cgacattatt
     2221 ccactgactc actgcccaca cgatgttcag gatatgctgg gttatcacca tcctctggca
     2281 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatatagc acggaaatta
     2341 ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg
     2401 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     2461 tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac gcgagccgca
     2521 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     2581 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     2641 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc accgtggttt
     2701 tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga agttatttat
     2761 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     2821 gaaagaggac tgacgaacgc gcctgatgaa gatccggacg atttaagcac gggatattac
     2881 ggttcagcgt accgctcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     2941 gcggcagccc gtcgagggat tatttctgac aggtttgtag aagcaatttt gcagttttgg
     3001 cgcgaaagat gagcgttcat taatttgatt tattcgcatc catcagcgct tttaactcct
     3061 ccggcgttga actgcacccc aaaagttgga cacccaactg agtcggtgga ggagtattcg
     3121 tgattaaggg gcggagatta tagaccgtgt taattctcaa ccatcaatcg ggaaagctta
     3181 gcaaaaatcg aactcttact actttcgccg ctcctgagca tatccagggt gaagctattg
     3241 aggtaagcac cagttaacgg gtggttaaat acatcgacgg ccttaccttg agcatcccat
     3301 atcagcagat tttctaccag cttaggccgt gcaatagtag gatttgt
//
LOCUS       DAJOTB010000280         3337 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJOTB010000280 DAJOTB010000000
VERSION     DAJOTB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.282"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1050..1385)
                     /locus_tag="OHN28_004833"
     CDS             complement(1050..1385)
                     /locus_tag="OHN28_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300075.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     PFYINVTGL"
     gene            complement(1416..1775)
                     /locus_tag="OHN28_004834"
     CDS             complement(1416..1775)
                     /locus_tag="OHN28_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCQ2300076.1"
                     /translation="MNRKLLALFMAAITLPVAAAQYVKPGAVLTLHPGQNNAEFNINA
                     STGDGSGVCNMEGTAQQIAAGESQKRRWVWNDNASECVAVISELTNGKASIMTRGCEG
                     YCGASAAGAMDGLFNKK"
     gene            complement(1790..2317)
                     /locus_tag="OHN28_004835"
     CDS             complement(1790..2317)
                     /locus_tag="OHN28_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300077.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(2329..2598)
                     /locus_tag="OHN28_004836"
     CDS             complement(2329..2598)
                     /locus_tag="OHN28_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2300078.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
     gene            2720..3208
                     /locus_tag="OHN28_004837"
     CDS             2720..3208
                     /locus_tag="OHN28_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300079.1"
                     /translation="MSSKRTVSAIPTHPEASVEEAKDWLTAEEFTHIEGMPGTARGAR
                     MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGEAFTLNDSDRQLSLW
                     VQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKLPEDERKRLFEKLNG
                     KV"
BASE COUNT          874 a          752 c          775 g          936 t
ORIGIN      
        1 cccaattgcc tgatacctta caaatcatag ttatagagaa taaagaaccg ccggtatcaa
       61 tcatgaataa agttcattac gaatggtttg cgggtgaaca tgcaatggat ggagagcgca
      121 gagcccttat cccgctgccc tcaaaaaata gtgctatttt aatgaagtaa cttaggatgg
      181 gtagccatca aatcgcagtt atacgattgg tggggtttgt aaaagcggta gtcttgatta
      241 ccactgcatt cattctacaa gcacatactt ccctaggttg ctgtagttat ccgattacac
      301 gccttttctg aaagcagcct cagggagccg gataaaaccc gttaaaagtg gcttgcctat
      361 actgctgcgc ggcgctgacg ctttagaggc cccaggattt aggcaaatca atgtagttag
      421 ctcagtagca ggtcatatct gggctataaa tggcattacg tgacactgaa agagcttccc
      481 agtggcatcg tatttggtga gccccaacct acaagacggg cggggatagc cgtgattggc
      541 acccacagca aggcgataaa gatgttcaac ggagcggctc agcaggtatg gggatggtag
      601 gatttagtct tcatatgcct gtggaattaa tcaaccctca gagcccgaca cacccggcat
      661 tgcttacggt ataagactga ttgagaaggt gtttgacgtt tttgtatccc agtctgtcgg
      721 taaagtaaac acagtgatca gtaactggta gtactggaag tgattgcggt cgtaatgctg
      781 attttgcacg ctacgacagg tatattctgt ttggtatctg tagaggttgt gatggcgctg
      841 accttgcaca tgccaggtac gttagactta ataataaagg gagtattagc aggagggatt
      901 ttttcggact cagttgtatt gtgtgtaacg agccaactaa atgtgataat agctataatg
      961 gtaaatatga tgatcttttt catagggttt ccttttggaa taaaacatga attatttcac
     1021 ccaaaaggaa cccggatgtt attcagtgat tataaacctg ttacgtttat ataaaacgga
     1081 cctttaccct catcagtttt ctgaatccgg taggtgattg gtagagaatt ttgcaaatct
     1141 ggttttgtaa acgttaaatc agcagcgcac tgataaacat ccagatctga ttcatggcta
     1201 attgttctga cattttctac agaaatacgc acatccttat ccagtttcat ccctttctcg
     1261 attgtctttc ttgcaatatc cccgaccaga ttttttgcat catcactact acattttggc
     1321 gttttctcgc cgcagccagc cagcaaacca gcagcaatta ataccagaag tattgctctt
     1381 ttcatctgta gttccttcgt aaattaatga tacttttatt tcttattaaa tagaccatcc
     1441 attgcgcctg ccgcagaagc cccgcagtat ccctcgcaac cacgtgtcat aatgctggcc
     1501 ttaccgtttg tcagttcact aattactgca acacactcgc tggcattgtc attccagacc
     1561 cagcgacgct tctgactttc tccggcagct atttgctgtg ctgtcccctc catattgcat
     1621 acgcccgaac catcccccgt tgaggcatta atgttgaatt cagcgttatt ctgtcccgga
     1681 tgcagggtta gaaccgctcc gggttttacg tactgcgctg ctgcgactgg tagcgttatg
     1741 gctgccatga ataaggccaa cagttttctg ttcattaatt aattccctgt taagtgatac
     1801 ggatgttttt ggtgattttc cccagtgcca gcggtctgcg tttttccaca aagaaataaa
     1861 cgacgttgaa tgcagacttg cgatgattcc ctcgaccaag agagggccag ttgcggaagg
     1921 attcaatctg ttcttctgaa tagggtgtgt cagagaaatc aaacagcaca agcaaggctt
     1981 tgccgtcatg tttcatcaat ttctttactt ctgtactgtt gacggtcgca gagacattgg
     2041 aacgtgccgc agttggttta cgtactgcaa actcaacggc cacatcgtca atgacaaaat
     2101 caatgtaacc gtgcccggat acagttcctg gcagtttgct ttttacttcc ggtgagacgt
     2161 tatctgcaaa ccaacccagc aaatagcaac gtaccagagg cagtaattcc cgctcgctac
     2221 attcgttcag acgcagggtt ttgacgaact ccctgcgata aagagaactg aaaaaatact
     2281 caaagcattc cgttatttca gtgatggtgg ttgccatggt tgcatccttt acctgctatt
     2341 aggtccattt ttaagggcat acctttccgg ccataatgtt tccggtgtcg tatccagagc
     2401 cccggcaata atgcgctcag ctctgggata atgacgctct aaggcgttat ttaatgtccg
     2461 tggcgcaagg ccgtgactgc gtgacagtaa cgccagcgtt aaccctcgct tgtgaagtgc
     2521 tgccacaata tcggcacgat gccagttccc tgaacttcct ttgatttgct ttttctgcga
     2581 tgctgttgag ttacgcacag tattaactcc atggaatcag tacatggata ttaaatgcga
     2641 ataaaagata cctttcaagt tcttttggta tctatttatt ggttttcata ttaacgcatt
     2701 gatatctggt gaaatttata tgagcagtaa gagaacagtt tcggctatac ctactcatcc
     2761 tgaagcttct gttgaagagg ctaaagactg gctgacagca gaggaattca ctcatattga
     2821 ggggatgccg ggcactgcgc gtggcgccag gatgcgcttg gaaaagctgg catccctgca
     2881 cccggaaata cgtcgtaaaa ggactgccgg gaaaggtgtg gtttaccata ttagcgcagc
     2941 cacaatgtcg cttgatgatg agagaaacag ggctaagggc gaagcgttta ctctcaacga
     3001 cagtgatcgc cagctaagcc tctgggtgca gctgtatcgc aatatgtcgc cagcttcgcg
     3061 ggcgcaactg atggagcagg ccctcaggct ggtaaaggac gatctgatgt ctcaggggcc
     3121 caatgtttct gaactggcgg tacaaatcat gaagctgccc gaagatgagc gtaagcgcct
     3181 ctttgaaaag ctgaacggga aggtgtgata gcgtcagttt tccgccatgc cagacagaag
     3241 gaaccacccc ggaatcctga cctgtctgac atggcagaat ggaaagtatg ccatcactta
     3301 ataatgaata aaaacctatt gccagttatt tcattgc
//
LOCUS       DAJOTB010000281         3314 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJOTB010000281 DAJOTB010000000
VERSION     DAJOTB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.283"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1195
                     /locus_tag="OHN28_004838"
     CDS             <1..1195
                     /locus_tag="OHN28_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCQ2300080.1"
                     /translation="LRNKDECSVSDLANYFSVTKETIRTDLAWLQKRGVVSRHHGGVS
                     LKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGKVCVLGSFNVDIVA
                     KVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKIGRDQFNHFARNHI
                     ESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSDNDIIQLSHFIAES
                     DVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPFVDIVTPNETEASA
                     MSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCAHIPAFSAVAVDTT
                     GAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPDISLVDARIQQQNV
                     AIQTF"
     gene            1221..2144
                     /locus_tag="OHN28_004839"
     CDS             1221..2144
                     /locus_tag="OHN28_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCQ2300081.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            2199..3152
                     /locus_tag="OHN28_004840"
     CDS             2199..3152
                     /locus_tag="OHN28_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HCQ2300082.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
BASE COUNT          918 a          670 c          856 g          870 t
ORIGIN      
        1 acttcgcaac aaggatgagt gcagtgttag cgatttagct aactattttt ctgttaccaa
       61 agagacgatt cggacagatt tagcgtggtt gcaaaagcgt ggtgtagtga gtcgtcatca
      121 tggaggcgtc tcattgaaaa aacatttaat gcaaaatgcg ctttcgcaac aagagtatgt
      181 cgatatgagt ttgcttatca agcaacaaca gcagggtatt ggctatttaa cctcgcttga
      241 tgataaagga cgccgaatgg taggtaaagt ttgtgtgtta ggatcattta acgtcgatat
      301 tgtggcgaaa gtgtatcgct tcccgaatga aggcgaaacg ttagtggcca gggaaactac
      361 catggggccg gggggtaaag gcgcgaatca ggcactagcc gctcatcggg caggtgcgca
      421 aatacacttc gcctgtaaga ttggtcgcga tcagtttaat cattttgccc gcaatcatat
      481 tgagtccatt ggtattggtt catttacttt ttatgagtct gaactatcta ccaccggttg
      541 tgcggtcatt tatgtaaatg atgaaggtga aaacatgatt gctatgtctc caggggcaaa
      601 tcatgaatta agtgataacg atattattca actttctcac tttatagccg agtccgatgt
      661 attcattgta cagatggaaa ataatcttgc cgctactcaa ctggcattaa aatgtgctca
      721 aaaaatgcag gtgacaacga ttttaaatcc tgcaccgtgg tccagtgatg tcgccacatt
      781 attacctttt gttgatattg tgacgccaaa tgaaaccgaa gcatcggcga tgtcaggtat
      841 ggtaattgac aatatttctg atgctgtgaa ggctgcgaag catatttata atgccgggca
      901 atgttcagta attattacga tgggaaaacg aggtgcatta atttatgacg ggcaacaatg
      961 tgcgcatatt ccggcatttt cggcagtcgc tgtagataca acaggtgcgg gtgatgcgtt
     1021 taatggcgcg ctcgccgccg cactggcgaa gggagaatca cttgttaaag cagcctggta
     1081 tgccagcgct ttcgcttcgc tggcagtaga aaaagaaggg gcagccaata tgcctgatat
     1141 ctctctggtg gatgccagaa ttcagcagca gaatgttgcc attcagactt tctgatgttt
     1201 cactgaacga aggatgaaat atgaaaacaa ttgaagggat tattccggta atgctaacgc
     1261 cttttacggc ggataataaa attgattacc tgggtcttaa acggctaatt gactggtata
     1321 tcgaaaacgg tgccgacgcg ttatttgccg tgtgtcagtc cagtgaaatg cagtatttaa
     1381 ctttagaaga gcgcgtggag ctggcgagct ttgtggttga atatagccaa caacgagtac
     1441 cggttattgc gtccgggcat atttctgatg atctggaaca gcaaattcag gagctcaatg
     1501 ctatggcggc aacgggcatt gatgcgctgg tgttagtgac gaatcatctt gatccacata
     1561 acgaaggtac cacggtattc ctccgtacgc ttgatgcgct attgatgtca ttacccgaaa
     1621 gtatggcgct gggtctgtat gaatgcccgg ccccgtatcg ccgcttatta accgatgagg
     1681 aactgcttta ctgcgcacaa agcgggcgtt ttcaaatctt aaaagacgtc agttgtgatc
     1741 tacctacagt gacgcgacgg gtgcaattaa ctcagcacac gccaatgacg attgttaatg
     1801 ctaatgctgc cattgcctgg gaggcgatgc ttgcaggttc gaaaggtttt tgtggcgtct
     1861 ttgccaactt tcatcctgac ttgtatcact ggctttggct tgaaggaaaa caacaccctg
     1921 aagaggcaaa acaactcgcc tggtttttat cgctgagtgc cgtcagtgaa aatattggct
     1981 atccgaaaaa tgccaaaata tttcatcagc agcgaggaac atttgactgt gttcattgcc
     2041 gggtaacggc tgacgatgtg ctgaaaaaat attgggggct ggaggtggtg ctaaaacaaa
     2101 ttgctgatgc cgcagatttc cagcgtcaac aactcaaata ttaatatccg acagcacttg
     2161 ctgttattaa aaaataatta agacaggagt ctacaaatat gaaacgaacc ctgatgagtt
     2221 gtgccgttgc ttcactgctg gtttcaggtt ccgcgctggc tgcatggcca gaaaaaccaa
     2281 tccaaatcat tgtcccctgg ggcgcgggag gaaataccga tacggttgca cgcctggtgg
     2341 cagaagggct gcaaaaagag ttgaaagtga atgtcaacgt ggtgaatcgc acgggaggtg
     2401 ctggggtggt tggccatgat gccattgcga aagctaaagc ggatggctat accctgggaa
     2461 ttgcgacggt agaaatcacc atgatgcacc atcaggggat gacggcgtta acgtatcaga
     2521 actacacgcc aattacacgg ctggcagtga atctgggagg cgtgcaagtc gcagcaaatt
     2581 cgcagttcga aaatatcgtc cagatgaccg actacatcaa ggcgaatcca ggtaagttaa
     2641 aagcatcggg tagcgggtta aattccggct ggcatctgaa cttgataggt atgctcgaca
     2701 gtatggggct tccgccttct gccgtgacct gggtgccttc tgaaggttcc agttctgcac
     2761 tgatggaact ggtttccggt ggtatagatt ttacaacgtc atcaccaggc gaagcgaaaa
     2821 gtatggtcga tgccaaaatg gtgcgtaatt tagcgaccat ggcagaaaaa tcagcagggc
     2881 tttataaaga tcttccagta ttccaacaag caactggcta taaatatcat ttggcgacat
     2941 ggaatgcctt agtggttccg gcgggtactc ctgaagaggt ctcacaaaaa ttaactgccg
     3001 ctatgcaggc agtgtttgcg caggggaaat tacaagaatt cgctaccaaa cagggtttcg
     3061 aagtttaccc gctgtatggc aaagagatga cgacatttat ggcagatgaa gatgccagat
     3121 acggaaaatt aatagccaaa atcaaaggtt aatcagacag ggcggtgaat atatcgccgc
     3181 caaattacag ggatagcatt atgcgcataa taccgataac gccgttattt ctgctgattt
     3241 ttgccatgtt tatttttggc tggagcataa cgcaatatgg aggcatatcg cagtatggcg
     3301 ctggatttat gcct
//
LOCUS       DAJOTB010000282         3309 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJOTB010000282 DAJOTB010000000
VERSION     DAJOTB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.284"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(94..390)
                     /locus_tag="OHN28_004841"
     CDS             complement(94..390)
                     /locus_tag="OHN28_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCQ2300083.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            614..1330
                     /gene="tonB"
                     /locus_tag="OHN28_004842"
     CDS             614..1330
                     /gene="tonB"
                     /locus_tag="OHN28_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCQ2300084.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(1370..1768)
                     /gene="yciA"
                     /locus_tag="OHN28_004843"
     CDS             complement(1370..1768)
                     /gene="yciA"
                     /locus_tag="OHN28_004843"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCQ2300085.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1873..2412)
                     /locus_tag="OHN28_004844"
     CDS             complement(1873..2412)
                     /locus_tag="OHN28_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCQ2300086.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2442..3185)
                     /locus_tag="OHN28_004845"
     CDS             complement(2442..3185)
                     /locus_tag="OHN28_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCQ2300087.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3213..>3309)
                     /locus_tag="OHN28_004846"
     CDS             complement(3213..>3309)
                     /locus_tag="OHN28_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2300088.1"
                     /translation="QQLPPLYKDMLFHTRPDAATRGLTRVQLPAN"
BASE COUNT          946 a          823 c          792 g          748 t
ORIGIN      
        1 gcttgtggtg gtgaatgcac tgaccgacct ataaggaaag gccaaacaag aacacggttg
       61 caaaaaccgt gcccttaaat attgaatttc tattcagaac actttcttaa atggtttcac
      121 tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc aggcctgagc
      181 tgcttccagc gactcaaatt cagcaataac ggttgagcca gtaaatcccg cagcccctgg
      241 atcgttactg tctaccgctg gcattggacc ggcggtcaac aaacgacctt catcatgcag
      301 taactgtaaa cgtgctagat gtgccggacg aacggaaagg cgtttttcga ggctatcagc
      361 tttgtcttga gcgtaaataa catacaacac aggcacaact ccttgttcgg gaaagttgta
      421 aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt tgttaagacc
      481 atgcataaag taaggtcaat tacgccaaaa atgacatttt cactgatcct gatcgtcttg
      541 ccttattgaa tatgattgct atttgcattt aaaatcgggg cctggttttt ctactgaaat
      601 gattatgact tcaatgaccc ttgatttacc tcgccgcttc ccctggccga cgttactttc
      661 ggtctgcatt catggtgctg ttgtggcggg tttgctctat acctcggtac atcaggttat
      721 tgaactacct gcgcctgcgc agccgatttc tgtcacgatg gttgcgcctg ctgatcttga
      781 accgccacaa gccgttcaac caccaccgga gccggtggta gagccagaac cggaacctga
      841 gccgatccct gaaccgccaa aagaagctcc ggtggtcatt gaaaagccga agccgaaacc
      901 aaagccaaaa ccgaagccgg tgaaaaaggt ggagcagcca aaacgcgatg tcaaacccgt
      961 agagtcgcgt ccggcatcac cgtttgaaaa tacggcaccc gcgcgcccga catcaagtac
     1021 ggcaacggct gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag
     1081 ccgtaatcag ccgcagtatc cggcacgagc acaggcattg cgcattgaag ggcaggttaa
     1141 agttaaattt gatgtcacgc cagatggtcg cgtggataac gtacaaatcc tctcagccaa
     1201 gcctgcgaac atgtttgagc gtgaagtgaa aaatgcgatg cgcagatggc gttatgagcc
     1261 gggtaagcca ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg gcactaccga
     1321 aattcagtaa gcataaagtc aaaagcctcc gaccggaggc ttttgattat tactcaacgg
     1381 gtaaggcgcg aggttttcct tcgggatcaa ccgcgacata cttaaataat gcttctgttg
     1441 ctttatagcg ttgcccgatt ggttctgacg ccactttttt cacccacact tcaatattaa
     1501 tgctgactga tgtcgtccct ttctggacac agcgtgcata gcagcacacc acatcgccga
     1561 ccgcaaccgg ccgtaagaaa gtcattcctt caacccgcac ggtcactaca cgaccgtggg
     1621 caatttcttt cgccagaata gcgccgccaa tatccatttg tgacattaac caaccaccaa
     1681 agatgtcacc attggcattg gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc
     1741 cctgagggac gttatgtgct gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc
     1801 gcgatgctac tatgattttc ctgaggagaa cagaggagat acagcgccag ccccgaagga
     1861 ctggcagtct ggttaggatt tatcttcctg cggcatgtgg cggtagatat agataccgct
     1921 taacaatgtg aagattaagg taagggcggt caggccaaag actttaaagt tgacccaaat
     1981 attttgcggc agccagaatg cgatgtagat gtttgccagg ccgcaaagga taaagaaaac
     2041 agcccaggcc agattcagct tcgaccatac cgattgcggc agggtgagtt ctttacccag
     2101 catccgctga attagcggct ttttcatcac ccattgactg actaacaggg cacccgcaaa
     2161 cagggcataa atgacagtaa ccttccattt aataaactca tcattgtgaa agaacagcgt
     2221 caagccaccg aagacgacca ccagaacaaa agtgatcagg gccatcttct caaccttacg
     2281 aaagcgaacc cagctataga taagcacaat cgccgtggcg acgatcagcg ccgcagtagc
     2341 cgcatagatg tcataaatct tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag
     2401 aaactgcttc attttatgat tccgtaatca aatgcttaag gttattggcg aatcaacata
     2461 tacaggcgga acagatagat gagcaatacg gctgaaatca ggttgctcaa ggtgttcgcc
     2521 agtacggcac caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt
     2581 gtttttgcca gcaaccagct cagtactgcg ggtgccacca gacgcatatt cgcccatgtc
     2641 agccgcatac tgctacgcat cgaggcaaaa acgcccattt tgtcctgtac cagcatcacc
     2701 ggtgccagag ccagtaaaat ggccatgata attcccggaa cgaccaccag catgatgcca
     2761 atctgtacta aaagggtagt cagaaaaatc agaataaata actttggcaa tatcggcgca
     2821 ctggcaccaa tagctcgcag cgcactgact ctctgacccg cagacaccag ctggataatt
     2881 aatattacgc ccccggcgag aatggcgtta ccgattaatc ctgaaaaagt ggacgccgct
     2941 gaagcctgca gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac
     3001 ccactactgc cgctaacggg tacgccgtca ttgagctgcg caagctgtgc atcactgggt
     3061 gagaaaacat gccctaacac cactgtgata aacgcacata gcaacgatac caacagaatg
     3121 gtcataaatt gattacggaa gaaatttccg gtgtcacggt atacggactg cgccgtgata
     3181 gacatgcact ctccttgagt tttatgcagg tgttaattag cgggcaattg taccctggtt
     3241 aagccccggg tggcagcatc aggtcttgta tggaaaagca tatctttgta aagcggaggt
     3301 aattgctgg
//
LOCUS       DAJOTB010000283         3282 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJOTB010000283 DAJOTB010000000
VERSION     DAJOTB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.285"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..852)
                     /locus_tag="OHN28_004847"
     CDS             complement(<1..852)
                     /locus_tag="OHN28_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2300089.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQAISAVYSHIRGEKRSTRDKNHAQQAFGDAIGQSFDAAVARFSFPVWMLHLLR
                     KARDRIIH"
     gene            958..1917
                     /locus_tag="OHN28_004848"
     CDS             958..1917
                     /locus_tag="OHN28_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300090.1"
                     /translation="MTAQQSDALREIANKARVTTILQSKAWKDTQRILQRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIIALELKPDTRIKVEVGQWYRITGKHLSLNVPPFML
                     IPRHIRRKVDGFRQSGKTTKKPAQQFTGSLYKALSGDIDSAKLDAWIAGLPLSALEGA
                     KEISSMGFNPWVVAKSVRRSASPTFELFYQEYQSLVLKSVFKTEPGFEQLLTRLSFIK
                     QSILRKQQYRVKSELLEILGNVLFFTLLYNQGCPGEFVNALVEKEATYLKASPGEEKV
                     KAYQKMINYIEEFCNKMTEKYLISAAKRHYQKKKITRIGSGES"
     gene            complement(1914..2645)
                     /locus_tag="OHN28_004849"
     CDS             complement(1914..2645)
                     /locus_tag="OHN28_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300091.1"
                     /translation="MFDQRNGGSLNSLNSRHIQDEDLPKWLKNKVAALWFCLAIRKIN
                     VSGYHYLELVSPRQISGDSVCVNGSASSSHEIRLKIIRRWINSVVRIQSEACAKQFID
                     VIYKNWKEIRNKNRAKWLKNNSEQIEWAWEYVTKRIPDLHVTGFCPDNTEEKHIALLV
                     LLNILNSEDKQSQLVSVCAEEDFCKKMHDAFRKRFIDGKKDKRVQINVKISPSAKGAL
                     DRLTRERKTTQQAILEQLILNRRLD"
BASE COUNT          928 a          708 c          784 g          862 t
ORIGIN      
        1 gtggataatg cgatcccggg ctttacgcag aagatgtaac atccagaccg gaaagctgaa
       61 cctcgcaacg gcagcgtcaa aactctggcc gatggcgtca ccaaaagcct gctgtgcatg
      121 attcttatcc cgggttgacc gtttttcgcc acgtatatgc gaataaacgg cgctgattgc
      181 ctgtggtggc agtgcgtgga tcatcagcga cacaaacgcc ggaaggttcc agccgacagg
      241 cgcggcaacg gagagtaccg gaaatgatga agggaacagg gtggccaccc gggctgttac
      301 tgttgccagt gagagttcct ggtgacggga cgggcatttt tctgtgtcgt tccattcttc
      361 agcaggaaac acgcgatcgg catggctgag aacaaacaga aaacgcgagg gatctgcacc
      421 ttcattcagt aaaaactgat gcatggcaat gtcggcagca tacgcacgtt catcagcacg
      481 caggatccag ataatcagat ccagttcggg cagtaactga cggtaaagct cccggtattc
      541 ctgatcatgc tgcggtgttt caccaatgcc gggcagatcg accagcgtca ttctgcgttc
      601 accgagctgc agggtaagac gatgagcctg gcgggtgcag ccgttcagag gatgtgtggc
      661 gcagatacgg gactgaaaaa tggcattaca cagactgctt ttgccggtgc cggatttacc
      721 cataatgcca atcacgggct cgtaatgaat aacaccgtgg agatgttcaa ggattttctg
      781 gcgtatttct tcaggaagaa acgtcagata tgatttcact tgtgcggaga gggaagaatg
      841 gctgaatgac ataccgttat cctgttgatt tcgctgaacc ggatcacact aatccggctt
      901 atcattcagg taatatgggc gtgaaaaaat ctggagtggc agagtgtggg aagtgagatg
      961 acggcacaac aaagtgatgc gctgcgtgag attgctaata aagccagagt taccaccatt
     1021 cttcagagta aggcctggaa agatacccag cgaatactgc agcgcagcgg actcgtgtgc
     1081 agagaacggt cagagccgtt tgatccggag aaacattttg actgttatac tgtgcgttat
     1141 ctgtacctgc ttaacataat agcacttgag ctaaagccag atacacggat taaggttgag
     1201 gtggggcaat ggtatcgcat aacggggaaa catttatcac ttaatgtccc gccttttatg
     1261 ctgatcccgc gtcatatccg gagaaaagtg gatggattca gacaatcagg taaaacaacg
     1321 aaaaaaccgg cccaacagtt cacgggcagt ttgtataagg ctttgagcgg ggatattgat
     1381 tcggctaagc tggacgcatg gattgctgga ttacctctga gtgcgctgga gggagcgaag
     1441 gaaatctcaa gcatgggatt taatccatgg gttgtagcca aatctgttcg tcggtctgca
     1501 tccccgacgt ttgaactgtt ttatcaggag tatcagtccc tggtactaaa atctgtgttt
     1561 aaaacggaac cgggatttga acaactttta acaagactga gctttataaa acaatcaatc
     1621 ctcagaaaac aacaatacag ggttaaatct gagttactgg agatactggg gaatgtgttg
     1681 tttttcacgt tgctttataa ccagggatgt ccgggcgagt ttgtaaatgc acttgtggaa
     1741 aaggaggcca cttatctgaa agcgtcccct ggcgaggaaa aggtgaaagc ataccagaaa
     1801 atgattaact acattgaaga gttctgcaac aaaatgacgg aaaagtacct gatatccgca
     1861 gccaagagac actaccaaaa gaagaaaatc accaggatcg gaagtggtga aagttaatcc
     1921 agtctacggt tcagaatcag ttgttccaga atagcctgct gtgttgtttt ccgctctctg
     1981 gtcagtctgt ccagagcacc ttttgcggat ggcgatatct ttacgttgat ttgtacacgc
     2041 ttatcttttt ttccatcaat aaaccgtttc ctgaaagcgt catgcatttt tttgcaaaaa
     2101 tcctcttcag cacagacact cactagctgg ctctgcttat cttccgaatt cagtatgtta
     2161 agtaacacta aaagggcaat gtgcttctct tcagtattgt cagggcaaaa cccggttacg
     2221 tgtaaatcag gaatacgctt ggtcacatat tcccatgccc attctatttg ttctgaatta
     2281 tttttaagcc attttgcacg atttttgttc ctgatttcct tccagttttt atagatgaca
     2341 tcaatgaact gtttagcgca ggcttccgat tgtatccgta ccacggaatt aatccatcgc
     2401 ctgatgatct tcagccgaat ctcatgggat gaggaggctg aaccattgac acaaacagaa
     2461 tctcctgaaa tttgacgagg cgaaacaagt tcaaggtaat gataccctga tacatttatc
     2521 ttccttatcg ccagacaaaa ccagagtgcg gcaactttgt tcttaagcca tttcggtaaa
     2581 tcctcatcct ggatatgacg tgagtttagc gaattcagac tacctccgtt tctttggtca
     2641 aacatgatac gggcttatcc cgtctgactg atcgttaatc ttatttcctc cttgcttaat
     2701 tgcttaccac taaagtgatt aaggtgcaat gaaatttaca aaaaaaactg tctgaatttg
     2761 cctgcaaaac aaaccgacga ccagattttt atgcaaacta ttcctaaact attcctgact
     2821 atatctaaaa catgctatca aaaattaaga taatcaccca ccccgttgct acttttacgt
     2881 tcatgctctc acacgtaaaa atggctccgc tctgtccgaa caatggagat aaaaacacgg
     2941 cctgctcatc cctgatgtga aattctgatg tgtatggcag taaaaaataa ctgacatgca
     3001 tgttatatgt gaagggctcg gagagacgac ataatcaata tggttgttct tctggttgta
     3061 aaacgcgatc gctttccggg atagatgacg acgcagttaa ctgacgaatg ggatgctccg
     3121 gatggtcttt tcagggagca cagagatacg gagtaagaaa aataattcca gagtaagaca
     3181 gtctgttttt ttaaccagca aagttccatg ccagatgtga aaacgggaaa ccaacattca
     3241 gtctgcatga gtgtctgaga atgttgctaa tgtcggtatg at
//
LOCUS       DAJOTB010000284         3260 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJOTB010000284 DAJOTB010000000
VERSION     DAJOTB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.286"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..614)
                     /locus_tag="OHN28_004850"
     CDS             complement(<1..614)
                     /locus_tag="OHN28_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCQ2300092.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSGSNTNTSDKLTIWNDLSPGTLLVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLF"
     gene            complement(805..1188)
                     /locus_tag="OHN28_004851"
     CDS             complement(805..1188)
                     /locus_tag="OHN28_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCQ2300093.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
     gene            1465..2112
                     /locus_tag="OHN28_004852"
     CDS             1465..2112
                     /locus_tag="OHN28_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCQ2300094.1"
                     /translation="MIQKANQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            complement(2409..3230)
                     /locus_tag="OHN28_004853"
     CDS             complement(2409..3230)
                     /locus_tag="OHN28_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2300095.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          992 a          722 c          625 g          921 t
ORIGIN      
        1 ctgaataaat caaagtgttc aaaaaattct tttattttcc gtttgactgg tttaattcga
       61 tcctcagatg ctctgtctgt aatacccaat tctctggcaa ttgttgcatg accaacaccc
      121 agcatataaa aagagaaaac aagcaatgtc ccgggggaaa gatcattcca tatcgttaat
      181 ttgtcagaag tatttgtatt acttccactc tgatttaacg caaaaaatgg ataatcagga
      241 aacggattaa tttgccataa aacaacagat tcgtcattat aaaaagaaac attctccatc
      301 cttatctgat agatttcgcc atgaaaatta aaacgacgac aaaatgcaga gccatgtcca
      361 agagcctggc attccagttc aagtgatgaa agaaaaattt caacctgcag acgtatataa
      421 ctctctccgg ataagggttt atcttctctg gaaaagagtt caataaaagc cccattggca
      481 taaaggattt tccggttttt gttacggaca caaaccggaa cgggagaatt tctgtagata
      541 ttctgaacgt taaggatgta tttatttaca gattgactgt taagtggcct ttctctacga
      601 tccagcgcac acataggaac ctccgttaag gattctatgg acagtcgatg cagggaggtc
      661 tgaactccct gcatcggcga tttttagttt caaattttaa cgtggcatta accacgtaat
      721 taatatacta attatctgac gtattgtcat ctttttgaat cataaaaatt cttatatttg
      781 aaccaactca aacttcattt tcatttattc atcatcattt tttggaaaaa accggtccat
      841 ctcaacagac actttctctc tgatatcgtc aaccatactg gcatattcaa acttcgggtt
      901 tccggagaca tgaggactga gagactcaat acctaaaatc tttgccaccg ttgactgtgt
      961 ttttacaaca cattcaagaa gaagtttatt aaactctgtc tggttaaacg cagactcttt
     1021 acgctccatc tgtgcatcat atacgcgaag ccccaattca agaagcattg aagcagttcc
     1081 tgaaaggctt atatctttat ctcttgctcc ctcctgacga cgcttttcaa caatcatgtt
     1141 aattttttca tgaacctcat tactgatata cagatttact ctggccataa gacagtacct
     1201 tttatattct gaaataagtg ttttatttca tttactcata gtttggtctg aaaaaagaag
     1261 agcaccccta gcagcgcccc tagggtcata ctataaaaaa acgcacggcg ctgctagggg
     1321 cggccctaat atatccaaat gtttgtcata aaatttgtca gtactgaccc tagcaagggt
     1381 cgccataggg tcgccatagg gtcgtcaacg accctattta ataatgtaaa ataaattaaa
     1441 atacattatt taaaacataa actaatgatt caaaaagcaa atcagcaaaa acttgttttt
     1501 gcgtggggtg tggtgctttt ggtggtgaga accaccaacc tgttgagcct ttttgtggag
     1561 tgggttaaat tatttacgga taaagtcacc agaggtggaa aaatgaaaaa atggatgtta
     1621 gccatctgcc tgatgtttat aaatgagatc tgccaggcca ctgattgctt tgatcttgca
     1681 ggccgggatt acaaaataga tcctgattta ctgagagcaa tatcatggca agaatcccgt
     1741 taccgggtta atgccatcgg tattaatccg gtaacgggat atggcagcgg actgatgcag
     1801 gtagattccc agcattttaa cgaactggcc cgctacggaa ttaagccaga acatctgaca
     1861 acagatccct gcatgaacat ttataccggt gcttattatc tggcaatagc atttaaaaaa
     1921 tggggcgtca cctgggaggc cgttggtgca tacaatgcag gattcaggaa atccgaacgc
     1981 cagaaccaga gacgtcttgc ctacgcatca gaggtttacc ggatttatac cgggataaaa
     2041 agcagtaaag gtatccggct tccggccacg aagaaatcac ttccggaaat taacagcgtg
     2101 cagaacaatt aatcttacag acattgtcag ataccggtta tgccgcaaaa gcggcatgga
     2161 aggccacaca caaaaatatc atatgagtga caatactgac tggccgaagc cccgcgaagc
     2221 gggaacccat cttatatata cccggtaact gcgcagcctc ctgccagacc atttttcgcg
     2281 ggcacaatat ttacagacaa ccggaaagcc ggtcgatgaa attgaggtat atcagacgac
     2341 acagcaatgc cgaaaaatga taatataagc aaagccccga aaattttttc ggggctttgc
     2401 ttatattatc atgacttcca ctttctgaac tgttcggcaa tcacccataa cgccttgttg
     2461 attcttatat caccatcaat acctgtaatg gctcttgtcc tggtattttt cccggaggca
     2521 cttcgccccg ataatccacc ttttatcata ttctcctgaa cccgctgcca ggttgtccag
     2581 agatcgttct gtttgtcttc ccgacgacgg ggagtaatta tctgttcagg tgtcactggc
     2641 gttttatttt catcttcata cctgaccatc agtgcagctc tgccaaataa atgttgctcg
     2701 tcactgttaa gatgaatttc tttcatcgcc tccatattat cagtgacctt atcaaagaca
     2761 ccgagcactt catacgctcc ctcgataacc tgcccgacaa tatcaccttt atgtggaacg
     2821 cggatttcgc caaaattatt tccgcacacc aggccatttg tgcagacaaa acgaaaaatt
     2881 ccggggatca tctgataact ggatgaacca tcatgtgaat taagcaggat aatttcagga
     2941 acttcctgtc cgttaatatg cccttccctg cgaagacgta acatatgttt actgtattcg
     3001 cgacgtccca aatcacgaac ccgactctga caggcaaaga atggctggaa accttcatca
     3061 cgtaacttat tgatgatatt gattgttgga atatacgtat aacgttcact ccgggactca
     3121 tgtttatcac cggaaaatac cgaaggcacg aactgcatta attcatcatc cgttaaagga
     3181 cgttcacggc ggatggaatt ataccgacca aaacgggaag ctaatcgcat aacagctact
     3241 cctttatgaa agaataaatt
//
LOCUS       DAJOTB010000285         3218 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJOTB010000285 DAJOTB010000000
VERSION     DAJOTB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.287"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(71..598)
                     /gene="mobB"
                     /locus_tag="OHN28_004854"
     CDS             complement(71..598)
                     /gene="mobB"
                     /locus_tag="OHN28_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCQ2300096.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(580..1164)
                     /gene="mobA"
                     /locus_tag="OHN28_004855"
     CDS             complement(580..1164)
                     /gene="mobA"
                     /locus_tag="OHN28_004855"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCQ2300097.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1234..1503
                     /locus_tag="OHN28_004856"
     CDS             1234..1503
                     /locus_tag="OHN28_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCQ2300098.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1580..2566
                     /gene="srkA"
                     /locus_tag="OHN28_004857"
     CDS             1580..2566
                     /gene="srkA"
                     /locus_tag="OHN28_004857"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCQ2300099.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2583..3209
                     /gene="dsbA"
                     /locus_tag="OHN28_004858"
     CDS             2583..3209
                     /gene="dsbA"
                     /locus_tag="OHN28_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCQ2300100.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          811 a          780 c          787 g          840 t
ORIGIN      
        1 ggcctttttg catatgtgcg gaataaatgc cggatgcgac gctggcgcgt ttgcttcagc
       61 ctattcttgc ttatccgtcc tgcttttgca tccactccac cacaaaatca gccagcccct
      121 caacatcatt aatatccagt aacgcgacat caagattaag cggcacatca ctggctacag
      181 caataacatg cctgtctatc actaattctt ccggtcgatg tccggctcca tcgcgaaaca
      241 gcacaatctt tgcgatctct tcatgcttaa acccttcgac cagaatcaaa tccagctttg
      301 aggtatccat tcgacttgcg agaaaatgta gatccagctc ttcttcgtct ggtgtttccg
      361 tcatcaaggc ccatcgctgc tggctggcaa cgatggtttg cgccgcgcca gccttgcgca
      421 gctcatagct atctttgccc ggtttatcaa catccatatc atggtgcgta tgcttaatca
      481 gccccggacg gatccctctg gcgcataatg ccgggatcaa ttttttcagc agcgttgttt
      541 ttccggtgcc actccatgca gcaaaggcga gtaacggtat catcgttttt cctgccatcg
      601 ggcaagctcc tctggcgtat tcacgtttat aaatgcctct ttacgatcgc tgaaatcaac
      661 cgcatgaccg ccagccagac gcataaatgc cataacccgg cgttctcctg cttgcagata
      721 ttccagtaat aaaggctcaa ttgcgcggtt taccagagca atagtcgggt gatcgcgttc
      781 accgtcgtgg acccacacga caggcgcatc tttgcgctga tgagtaagcc gggcggctaa
      841 atcatgggga atgtaaggcg tatcgcacgg gcaaaacaaa aaccactcac ccgcttcctg
      901 ctgcattact gaaagcattc ctgccagagg gccagggtaa tccgccagtg aatcttcaat
      961 cactttcaga ccgcttacct gatagatttc ctgatgacga ttagcattaa ccacgacgtg
     1021 agaaagctgc gtcataagcg cgtcagcgac atgttgccat aatggtttgc cgtttaattc
     1081 aagcaatcct ttatctacgc cgcccattcg tctggcttta ccgcccgcca gcacaacgcc
     1141 tgttatcgtc gtcatcagat tcaccgatat cgcctctttt attgtgggat tgaccctgct
     1201 aacgtgtctg tctcaagagt aaggagcatt actatgaaat gtaaacgtct gaatgaagtt
     1261 attgaactcc tccagccagc ctggcaaaaa gagccagacc ttaacctgct gcaatttttg
     1321 cagaaactgg cgaaagagtc aggttttgac ggcgaactgg cggatttgac ggatgacatt
     1381 ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg cggtgatccc gggtttgcag
     1441 aaagattatg aagaagattt caaaacggcg ctattacgcg cacgtggcgt aattaaagag
     1501 taaaagcttg taagcggcgc aaccaaaatc atcgtgaaat gatatccttc atcattcgta
     1561 atgttttccg gatgatggga tgaacaacag cgcttttact ttccagacac tacacccgga
     1621 taccatcatg gacgctctgt ttgagcatgg gatccgggtg gattccggtc ttaccccgct
     1681 taacagctat gaaaaccgtg tctatcaatt tcaggacgaa gatcgtcgac gttttgtcgt
     1741 caaattttat cgccctgaac gttggacagc cgatcaaatc ctcgaagaac atcaatttgc
     1801 gttgcagctg gttaacgatg aagttccggt cgcagcacct gtggccttta atggtcagac
     1861 tttattgaac catcagggat tttatttcgc tgtttttcca agcgtcggtg gtcgccagtt
     1921 cgaagctgat aatatcgatc agatggaagc ggttgggcgt tatttagggc gtatgcacca
     1981 gacggggcgc aaacagcttt ttatccatcg cccgaccatc ggtttgaatg aatatctcat
     2041 tgagccacgc aagctgtttg aggacgctac actgatacct tccgggttga aagcggcatt
     2101 cctgaaagcg acagatgagc tgattgccgc cgttacagca cactggcggg aagatttcac
     2161 cgttctgcgg ctacatggag actgccacgc cgggaatatt ctctggcgcg atggcccaat
     2221 gtttgttgat ctggatgatg cacgtaatgg tccagccgtt caggatttgt ggatgttgct
     2281 caatggcgat aaagccgagc agcggatgca actggaaact attattgagg cttatgaaga
     2341 atttagcgag ttcgacaccg ctgaaatcgg actgattgaa cctttacgcg ccatgcgttt
     2401 ggtttattat cttgcctggc taatgcggcg ttgggctgat cccgcgttcc cgaaaaattt
     2461 cccgtggtta accggggaag attactggct acgacagacg gcgactttta tagaacaggc
     2521 aaaagttcta caagaacccc ctttgcaatt aacacctatg tattaatcgg agagagtaga
     2581 tcatgaaaaa gatttggctg gcgctggctg gtttagtttt agcgtttagc gcatcggcgg
     2641 cgcagtatga agatggtaaa cagtacacta ccctggaaaa accagttgct ggcgcgccgc
     2701 aagtgctgga gtttttctct ttcttctgcc cgcactgcta tcagtttgaa gaagttctgc
     2761 atatttctga taacgtgaag aaaaaactgc cggaaggcgt gaagatgact aaataccacg
     2821 tcaacttcat ggggggtgac ctgggcaaag agctgactca ggcatgggct gtggcgatgg
     2881 cgctgggcgt ggaagacaaa gtcacagttc cgctgtttga aggcgtacaa aaaacccaga
     2941 ccattcgttc agcatctgat atccgcgatg tatttatcaa cgcaggtatt aaaggtgaag
     3001 agtacgacgc ggcgtggaac agcttcgtgg tgaaatctct ggtcgctcag caggaaaaag
     3061 ccgcagctga cgtgcaattg cgtggtgttc cggcgatgtt tgttaacggt aaatatcagc
     3121 tgaatccgca gggtatggat accagtaata tggatgtttt tgttcagcag tatgctgata
     3181 ctgtgaaata tctgtccgag aaaaaataat tcaatgta
//
LOCUS       DAJOTB010000286         3162 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJOTB010000286 DAJOTB010000000
VERSION     DAJOTB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.288"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(49..810)
                     /gene="kduD"
                     /locus_tag="OHN28_004859"
     CDS             complement(49..810)
                     /gene="kduD"
                     /locus_tag="OHN28_004859"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCQ2300101.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(840..1676)
                     /gene="kduI"
                     /locus_tag="OHN28_004860"
     CDS             complement(840..1676)
                     /gene="kduI"
                     /locus_tag="OHN28_004860"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCQ2300102.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1963..3144)
                     /locus_tag="OHN28_004861"
     CDS             complement(1963..3144)
                     /locus_tag="OHN28_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCQ2300103.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          832 a          873 c          714 g          743 t
ORIGIN      
        1 ataaaaaacc ctgccatgcg acagggtcat aaaagtaaga ggaatgaatt aacgcgccag
       61 ccaaccgcca tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag
      121 gaacaccacc ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc
      181 gagaatttcc gcgctacgtt gttcatctgc ccgcagttgt tgagtattgt tggtcgccat
      241 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa
      301 tcgcgtcacg cccatcacac cgctttttga tgcggtataa gaaggcacac ggatcccgcc
      361 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
      421 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
      481 gtcccagtct ttttcgctga attcgagagc gtcttcgcgg cgaatcaatc cggcgttatt
      541 caccaggata tcaatatgac caaactccgc taccgcgcga tccagcagtg ctggaatgcc
      601 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
      661 cttgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
      721 cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt
      781 accttcgaga gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac
      841 tagcgcaaat ctttaacggc cacatggtcc atatcatcaa aaacctggtt ttcaccgacc
      901 atgccccaga taaaggtata agctttggtt ccgacgccgg aatggatcga ccagctcggg
      961 gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ttgccccatc
     1021 atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc
     1081 tcgtgggtgt gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc
     1141 atactcaatt ggcaggtttc cagcacatcc gggacaaaat atttgttaat cgtgcgacgg
     1201 ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg
     1261 gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta
     1321 tcaatactgg caaagacaac ttcttttgca cctttaccaa catacagggc gtcgcggtga
     1381 ccgatttcat agcattgacc atcgacagta atcgtaccgg caccgccaat attgataaca
     1441 cctaactcgc gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca
     1501 acggaaaccg ttttagttac cggcataatg ccgccaacaa taatacggtc aatgtggctg
     1561 taaaccatgg tgtactcatc ggcgacaaat accttttcaa ccaaaaattc attgcgcagc
     1621 ccctgggtat ccagcgtttt tgcgtgcgcg ctgtggatgc tctgtcttac gtccacatca
     1681 acctccgata atcagtgaag cgtgaaaata gaaaacaata ataaaacact gtttcgtttt
     1741 ctctatgagg ggatgatatg cccatgggat agggttttca attaaaaata aaacaatgtt
     1801 tcattttcaa gttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat
     1861 ggaaaaatca gggagccctg aaaaggtatg tcgactcacc agccatcatt attcaacaga
     1921 ttaatgacgg cagatattta tcggtaagga ggtaatgcag agctactcgt cacgttcaat
     1981 ggtcaatgcc acaccctggc ctccgccgat acaaagcgtt gccagtcctt tgcgggcatt
     2041 acgtttcacc atttcatgaa caagagaaac caggattcgg caaccggaag cgcctatcgg
     2101 gtgaccgagt gcgatcgcac caccattaac attgacccga cgctcatccc attcaagcat
     2161 cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc
     2221 agccaactgc cagccaacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc
     2281 catcaatgcc ggatccacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa
     2341 attcaacgct cgtgctttgg cttcgctcat catcattact gcagctgccc catcgtttat
     2401 ggatgatgca ttacccgctg tcacagaacc gagactatca aatgaaggat ttaaacgggc
     2461 taagccttct gcgctggtgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg
     2521 accgttactt tgggttatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc
     2581 cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc
     2641 atattcgcga gccagatttt cggcggtgac accaatatga taatcattga aggcatccca
     2701 caacccatca tgcacaagac tatcaaccaa ctggctattg ccaagctgtg cgccggtgcg
     2761 gctatcagtc agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat
     2821 atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa gcccggaacc
     2881 acaaacgtca ttaatagtga ttgcagaaac gctattagga agaccacctt taatagccga
     2941 ttgtcttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa tcacttcatc
     3001 caccgcatat gcaggaacgc cggtacgttc tattaacgct ttgacgacca ggctgccaag
     3061 ttccacggcg gaatgacccg ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc
     3121 cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa tt
//
LOCUS       DAJOTB010000287         3103 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJOTB010000287 DAJOTB010000000
VERSION     DAJOTB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.289"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            13..1155
                     /locus_tag="OHN28_004862"
     CDS             13..1155
                     /locus_tag="OHN28_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCQ2300104.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1628..1729
                     /locus_tag="OHN28_004863"
     CDS             1628..1729
                     /locus_tag="OHN28_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300105.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2094..2357
                     /locus_tag="OHN28_004864"
     CDS             2094..2357
                     /locus_tag="OHN28_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCQ2300106.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2357..2497
                     /gene="ykgO"
                     /locus_tag="OHN28_004865"
     CDS             2357..2497
                     /gene="ykgO"
                     /locus_tag="OHN28_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCQ2300107.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2567..2757)
                     /locus_tag="OHN28_004866"
                     /pseudo
     CDS             complement(2567..2757)
                     /locus_tag="OHN28_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          911 a          675 c          651 g          866 t
ORIGIN      
        1 tgaggtattg ttatgacaaa taaacaccca tcattgtttt ctccttttat gctgacagaa
       61 aaaataaagc tacgtaatcg tatcgttatg gctcctatga caacctggtc agctaatcct
      121 gatggaacca tctctgagca agaactcgaa ttttataaac gtcgctcaca aaatgttggt
      181 ttagtaataa caggatgtac ctatgtcacg ccaagcggca ttgggtttac tcatgaattt
      241 gccgcgtatg acgaccgttt cattaacagc cttgaaaagt tagcagctgc agccaaaagt
      301 ggcggtgctc cggctatttt gcagattttc cacgccggta ataaagccat cccggagctt
      361 gtgccaaatt acgatgttat tagtgccagc gcatcaagcg taaaatctgg tgactttatg
      421 aagagagtgg tacaaagcag ggaaatgact gaaaacgaaa ttcaggaaac cattagagca
      481 ttcggagatg tcacaaaaag ggcgattaaa gcaggttttg atggcgttga acttcatggt
      541 gcgcacggat tccttctgca gaatttcttc tcccctctat ttaaccagcg taatgaccga
      601 tggggaggag atcttgaagg acgtatgcgc ttcccgctgg ccgtattaca ggaagtaaaa
      661 aatgtagtct atgaatatgc gacaaagccc tttgctatcg gttacagaat atctcctgaa
      721 gaatctgtaa cagggggact gcggatagag gatacttata aactccttga tcggctcatt
      781 tcatccggta tttcgtatat acatacctcg ctggtcagta ttaacgatag ctaccctgtt
      841 gagtcgccaa atggtccccg aactattgaa ttgattttaa atcatattgc aggcagagtg
      901 ccggtaatcg ccgcaggtaa aatacgcaca ccgtctcagg ctcaagaagc aatttccgct
      961 ggcctgccac ttgttgcgat cggtaaaggg ctggtgataa atccagaatg ggtgacttta
     1021 gctgaatcag gtagaagtca tgaaattcaa acagccctta atccacaacg cgttcctgaa
     1081 ttaaccattc cggataaact atgggatcaa attcaggcat cgaaaggaac tggctggttt
     1141 ccgttaatgg attaaatatc gattactctc ccgagagtaa aactatcaaa tcaatgagta
     1201 accgatacag cctcgcaaat tctttcagat aattaacccg tcactgcggc acgttctctc
     1261 gcaaggcgtg cctgcagtgt aatccagtat gtatccggca tagttgacgg tttaatagac
     1321 tcaattcctc tccctggtta cctacaacga tatcttgtat aacaccccag gagatacgac
     1381 gttacgatga gatgcgatat gctcctcagc gccaatcaac gccaggactg ggccagtctt
     1441 gagatcagac tcattcccgt aactgatttc aggcgacata tatcaatgac gttggggcaa
     1501 taaaagatag ccgatgtaat acatctattc aaatttgtga tacatgtcaa aaaattggtt
     1561 gaccaaactc gttatttata taagggcact tacgaagtgc actctttttt aaagcgagga
     1621 agtaccaatg aaagagaata aagtacagca aatcagtcat aaactgatta atatcgttgt
     1681 ttttgtcgca attgtagaat acgcctattt atttctccat ttctattaat aacggcaata
     1741 aactgttcac ttcagtgata tttaaaatat gcatcctctc ccttttttgt aagtaattat
     1801 tatatccgtg ggagaggaat acacattgtc aggtaatcaa tcatgctgca ataaatcatc
     1861 ggccagtaaa gtggagatag cctccattct cgaaaaatcc atactctcag cgaaaccatc
     1921 atcaatcact catccaggcg tttatgggag cgtcgccaat ggctgctaac aatgccagac
     1981 ttccccgttg cggaaattcc acatcccaca aatagtcaca gtgattgggt gttgaaatga
     2041 tccggatgag catgtatctt tacggttatg ttataacata acaggtaaaa atgatgaaac
     2101 ccaatatcca tcctgagtat cgtactgtgg tgttccacga caccagcgtt gatgagtact
     2161 ttaaaatcgg ctcgactatc aaaacagacc gtgagattga gctggatggc gtaacgtatc
     2221 cgtacgtaac aattgatgtc tcttcgaaat cgcacccgtt ctatacaggg aagctgagaa
     2281 cagtggcatc agaaggaaat gttgcacggt tcacccaacg ttttggtcgt tttgttagca
     2341 cgaaaaaggg ggcatgatga aagtccttaa ctctctgcgt accgcaaaag aacgccatcc
     2401 agactgtcag attgtgaagc gaaaaggacg gctgtatgtg atttgtaaat ctaatccacg
     2461 ttttaaggcc gttcagggtc gtaagaaaaa acgttgattt aaaattcgac gaattaacga
     2521 tatttgtctg attaataatc agaccggatt aatgttggtg tatttattac accagcatta
     2581 attttcctgg ggatgtatct tcctgttcat ttaaggtcaa ctgcctgacg tttctctccg
     2641 aatattccat tatattaatg ttgacttgtt gaccagcttc gccctgtatg ctggcatcaa
     2701 ctctctttta gactgaacac gccactcagt ttcctccctt tgcgacgcag cctgcattta
     2761 cactcaaact gttaagatga taaatgtggt aaatctgttg gtactgacat aaaaacgttt
     2821 acgccacagg aacggccaga tccatcggta accccatcgc cgacgttcga gtgccagtta
     2881 gagtaacgcg cacagatgac tgaatgcagt gccctggcaa agaggccatc gttcctgtga
     2941 caactggcag tcttcgttta acttcactta atttagctct tagggggctt accggacaga
     3001 tgacgtactt acacctgttt aattttccat cacttattgg aatgaacacc cataaccatt
     3061 ttgtgcggca tgttaatcca ttaaaacacc ttactgattg gca
//
LOCUS       DAJOTB010000288         3097 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJOTB010000288 DAJOTB010000000
VERSION     DAJOTB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.290"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1028)
                     /locus_tag="OHN28_004867"
     CDS             complement(<1..1028)
                     /locus_tag="OHN28_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCQ2300108.1"
                     /translation="MNSAYMQSLQTSHHFPADLTYRLFPSELAYLIDDLYESTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMNPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGYPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALVEGLNEHPEAGVISDEAVTFFRSYLKNYPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPDVFTNYINKNDVLAWGSGFLSRFLFSQTGSPSR
                     VRDYTRGEFRTKPTLEKFHKKINGFLLSHNINSPGMSTERKTLKLAKKALGEWQENQI
                     KIERKALAG"
     gene            complement(1355..2086)
                     /locus_tag="OHN28_004868"
     CDS             complement(1355..2086)
                     /locus_tag="OHN28_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2300109.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLHYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPA
                     SSDARPSSDDTPLSGGCSETRKASDKKPGEAAVLWIKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGAGRLLDGR"
     gene            2266..2439
                     /locus_tag="OHN28_004869"
     CDS             2266..2439
                     /locus_tag="OHN28_004869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300110.1"
                     /translation="MNGFKNDMDDEKSLKGKGATEHSNKTPEMQPERFLAMQINNNAK
                     TYNLITQLNHGCE"
BASE COUNT          929 a          734 c          642 g          792 t
ORIGIN      
        1 cctgcaagcg cttttctttc aatcttaatc tggttttcct gccactcccc caacgctttc
       61 tttgcaagtt ttaatgtttt cctttcggtg ctcataccgg gggaattaat gttatggctt
      121 aacagaaatc cgttaatctt tttatgaaac ttctccaggg ttggttttgt tctgaactcg
      181 cctctcgtat aatcccgtac ccgggaagga cttccggtct gactgaacag aaaccgggaa
      241 agaaatccgc ttccccacgc cagtacgtca tttttattta tataattcgt aaagacatcc
      301 ggctgggaca ttaacgaaaa tgtcagacgt ggcgtgatat ggtatttctc gtcagcccgt
      361 ccaaaatcaa acggttgtcc actccatgct ttattcaaca ggcccggata atttttcaga
      421 tagcttctga aaaaagtgac cgcctcgtca gaaataaccc ctgcctcagg atgttcattg
      481 agcccttcca caagcgcttt aagcgaaaca tcttcataga taaaattcgg tcgaaccgga
      541 cgtgtcggtt tatttctttc gtgattacgc agcgcctctt cttcctgttc ccccggatac
      601 ccccggttaa cagcctttct taaattggca gcaagcgctt tctgccgggt attccagatc
      661 tgtagttcag tcttataatc tttgtttctc tcttcgtatt gttgaatgag tcgatcggca
      721 aattcgtaac aggggttcat caccagtctg tttatcgttg tttttcccgc gcctggctct
      781 gcgattgcca acagataaag tgagcagggt tccggcatgt tggtgtgagg atgaacaacg
      841 tcaaccagtg actgacagga gagtgagagc gttgccagta cagtattaaa aatgagctcc
      901 agcggaagtt gggtactttc atataagtcg tcaatgagat atgcaagctc actaggaaat
      961 aatctgtagg ttaaatcagc tgggaagtgg tgggatgtct gcaaggattg catataagcg
     1021 gaattcattg taacggacct ccttcacata ctgtaatcag cgcaaaactt attgttcagt
     1081 tcaggaacat accggcatgg tgagtatgtt cgctgacgtc gataataaaa tggcttatct
     1141 ccattcacca gtcccggcgc atttatgatg ctgacaaaag taaaacaaca tgtgtgcatc
     1201 cagaagtctg gcatcgcagc gacaaatgat ggatgcatga aaaccaggaa acattgcatg
     1261 acttcaggac gactttttca gaaaacgagc taagggcgtc aaccacgccc ttactgtgac
     1321 atatactctg gcaccggatg cctttttaca gggttcacct gccatccagt agacgccccg
     1381 ctccacggct gcaaccataa ttacgttgac cagaaccgtc atgctgcttc gtctcatact
     1441 tcacgagata acgggctctc tccatggctt tctgcatcgc ttccacatca ccacgcttga
     1501 tccagagaac agcggcttca cccggctttt tgtctgaagc cttcctggtt tcagaacatc
     1561 cacccgacaa aggcgtatca tcggaagaag ggcgagcatc agatgatgcc ggtttacgga
     1621 aaggcgtccg cctggaaaaa tgaaccagtc cattaccctg ccagggctca agatgcagag
     1681 cgctacacca tgccagttgg atcagcgtcg ccagcgaaga aggaacggtg aaatcccctg
     1741 gcgagcacca ggtatctttg ttgagcagta atatcacgtg ataatgtttc ctgccttttt
     1801 ccttgccaaa ctcccgacac cagaaataat gcagggtggt tgcacgcaca cgcttgttgg
     1861 cacgtcgttt atgtcgctgg taagcctgaa tttttgcttt cagggatgca aaaaaacgag
     1921 aaatcaccgc tgaatctaaa tcggtatcca tgatggtggc aggcatatac tcagggaaac
     1981 gtaaatccac ccggattaac gagacggcac ggtgttgcgc cagggcactg ttgagaatac
     2041 tttcaaaacg ggataaccag taagcattgt gtattggttg attcatgatg agagatctcc
     2101 gttgatttgg attgacttca catcattaat caacgcagta aaactttacg gcatccggca
     2161 ttgagcaata ctacaactct ctgattaaga gtgattgaaa cggaacaaac catcccggaa
     2221 cgaaaagata atcctgcaca cagcagcaaa atacgcaaca acagcatgaa tggatttaaa
     2281 aacgacatgg acgacgagaa aagcctgaag ggaaaaggag ccacagaaca ttcgaacaaa
     2341 acaccagaaa tgcaaccaga acgcttcctg gcaatgcaaa tcaacaacaa cgccaaaacc
     2401 tacaacttaa tcacacaact caatcacgga tgcgaatgac acgtttaaaa cctgtttaca
     2461 gataacgccg taacacgatc atcaccatca ccatcaccat caccatcacc atcaccatca
     2521 gaatcgattt tctcatcccg aaaatgttac gtttcactac gcaccataaa aacgattaac
     2581 catcgataac gtaaacttca tctgaatcaa aacaccaaaa ccgttcatca cactttcgca
     2641 atgcaagaag aaacctcatg acgaaaacaa tgaccttaca cggaaaagaa aacgtcagga
     2701 atcgttaatg cctcaatctg attgttatca aaacatccag aaagtcattt aacagaatcg
     2761 ttgtaagcac atcgaaaaga acgtcagatt acttcaggaa agttcgggtt gatttagcgt
     2821 aaagatactt ttctcatgct aacgtcgaaa ctcgaaatct caggaaaggt taaacgcaac
     2881 atcagtaaaa tcgaatagca gattgattgc ttaaacagaa aggttttaca gcagctttaa
     2941 actgatttta gctaacgtaa agatttacta aactggatgt gtttcagaca aagcaatgtg
     3001 tacagcaatg cttaatgaaa acacttatag ggctgtttat gaaaacacgt gtatgtctgg
     3061 taaaacactt agtagaaaat acgtgtataa cacttac
//
LOCUS       DAJOTB010000289         3080 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJOTB010000289 DAJOTB010000000
VERSION     DAJOTB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.291"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            9..890
                     /locus_tag="OHN28_004870"
     CDS             9..890
                     /locus_tag="OHN28_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2300111.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1095..2564
                     /gene="lysP"
                     /locus_tag="OHN28_004871"
     CDS             1095..2564
                     /gene="lysP"
                     /locus_tag="OHN28_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCQ2300112.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2856..>3080
                     /locus_tag="OHN28_004872"
     CDS             2856..>3080
                     /locus_tag="OHN28_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCQ2300113.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVP"
BASE COUNT          651 a          743 c          852 g          834 t
ORIGIN      
        1 aagcgactat gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg
       61 gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct
      121 tgaccgacct ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg
      181 ttgttaatga acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg
      241 ttgaaatcga acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta
      301 ccatcggtaa ctacattcta cctgcagtta tcgcccgtta tcgccatgat tatccgcagt
      361 tgccgattga acttagcgtt gggaatagcc aggacgtgat gcaagcggtg ctggatttcc
      421 gcgttgatat tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt
      481 ggctggaaga cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg
      541 tcaccttaga acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc
      601 gggagattgt cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat
      661 taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt
      721 cgcgacgggt gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc
      781 tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg
      841 cgctacggcg ctttctggac tattgcgatc ccgcgaatgt accgcgttaa ggtgctgtac
      901 aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag
      961 aaaagagatt ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg
     1021 tgtatttctg ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg
     1081 gatagcattt ttgtatgggt tccgaaacta aaactacaga agcgccgggc ttacgccgtg
     1141 aattgaaggc gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt
     1201 ttgttgcctc tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata
     1261 tgctgattgg cctgatggtt tacttcctga tgaccagtct cggtgaactg gctgcatata
     1321 tgccggtttc cggttcgttt gccacgtacg gtcagaacta tgttgaagaa ggctttggct
     1381 tcgcgctggg ctggaactac tggtacaact gggcggtgac tattgccgtt gacctggttg
     1441 cagcacagct ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt
     1501 tgttcctcgg cgttatcttc ctattgaact acatctctgt tcgcggcttt ggtgaagcgg
     1561 aatactggtt ctcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc
     1621 tgatgattat cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg
     1681 gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct
     1741 tctctttcca gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga
     1801 aaaacattcc acgcgcggta cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg
     1861 cgatcctgat tatcagcctg attattccgt acaccgatcc gagcctgctg cgtaacgatg
     1921 ttaaagacat cagcgtcagt ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg
     1981 cggcggcggt gatgaacgca gttattctga cggcggtgct gtcagcgggt aactccggta
     2041 tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt
     2101 tcgctaaact gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg
     2161 ccggtttgtg cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga
     2221 acacctccgg gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgtt
     2281 tccgtcgcgg gtacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt
     2341 tcttcccact ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga
     2401 actacgaagc gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg
     2461 gtatcccgct gttcctgatt atttggttcg gctataagct gattaaagga actcacttcg
     2521 tacgctacag cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt
     2581 gctaagccct ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga
     2641 tataaaattt aacatttgga ttgataattg ttatcgtttg cattatcgtt acgccgcaat
     2701 caaaaaaggc tgacaaatca gaggctgttc cggctttctg ggatgatcac ctgcatacaa
     2761 ataagtccac cgcgatgctg ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc
     2821 attttatttt cgtagttacc tcatggagat atggaatgtt taggttgaac cctttcgtac
     2881 gggtcgggct gtgtttgtcc gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg
     2941 atggcgaaac gatggttgtc actgcatctt ccgttgaaca aaaccttaaa gatgctcccg
     3001 ccagtatcag cgtcattacc caggaagacc tgcagcgaaa accggtacag aatctgaagg
     3061 atgtcctcaa agaagtgcct
//
LOCUS       DAJOTB010000290         3071 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJOTB010000290 DAJOTB010000000
VERSION     DAJOTB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.292"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..316)
                     /locus_tag="OHN28_004873"
     CDS             complement(<1..316)
                     /locus_tag="OHN28_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCQ2300114.1"
                     /translation="MNPDYVQSLQTSHHFPADLTYRLFPSELAYLIDDLYESTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMNPCYE
                     FAD"
     gene            749..1405
                     /locus_tag="OHN28_004874"
                     /pseudo
     CDS             749..1405
                     /locus_tag="OHN28_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1499..2230)
                     /locus_tag="OHN28_004875"
     CDS             complement(1499..2230)
                     /locus_tag="OHN28_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2300115.1"
                     /translation="MNQPIHNAYWLSRFESILNSALVQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKSGGSAVLWVKRGDVEAMQKAMERACYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT          939 a          740 c          669 g          723 t
ORIGIN      
        1 gatcggcaaa ttcgtaacag gggttcatca ccagtctgtt tatcgttgtt tttcccgcgc
       61 ctggctctgc gattgccaac agataaagtg agcagggttc cggcatgttg gtgtgaggat
      121 gaacaacgtc aaccaatgac tgacaggaga gtgagagcgt tgccagtaca gtattaaaaa
      181 tgagctccaa cggaagttgg gtactttcat ataagtcgtc aatgagatac gcaagctcac
      241 taggaaataa tctgtaggtt aaatcagctg ggaagtggtg ggatgtctgc aaggattgca
      301 cataatcggg attcattgta atggacctcc ttcgcatact gtaatcaacg caaaacttac
      361 tgttcagttc agaaatatac cgacattgtg agtatgttcg ctgacgtcga taataaaatg
      421 gcttgtatcc attcaccagt cccgacgcat ttatgatgct gacaaaagca aaacgacatg
      481 gaggtgcctc agaagtctgg caccacagca acaaacgatg gggcatgaaa cacagggaac
      541 attgcgcccc tgagcgttga ggatcttcca ccttgtatcc tggttcttcg ttatcatgag
      601 cagcgaacag atttggcaaa gcgtatgcag cctggtgccc cttagggttt aagcccatat
      661 gtaagcaaat ggcgcatacg ctgagccact catctgaaac ccgaacagtt gtccgtactc
      721 cataacgcat tagcaagaat gggcaataat gagtctgcca aatccacttt gtgttgatac
      781 caatgtgtcc aaaacaacgc tggatattgc cgccagcaac actattgaac ggttttctgt
      841 tgccagtggt gctgacggcg ttgatttcat catcgctgaa ctgaggcagc acgatatggc
      901 ctttgtgttg atggaaggcc gccataacct gtgcgtttca gcccgaaggt tttgagatcg
      961 ccgtagttaa ccccaggcaa gctcgtgact ttgcccgtgc gatgggatat ctggcgaaga
     1021 ctgacccgca tgatgcgcgt tttttttgcc cagatggctg cagttattaa ttaccatccg
     1081 gaacgagacc gctttatccg gactttgccg gatgcagaac gtcaggttct taatgccata
     1141 gtcgtttgca gacaccagtt gatcgcaatg ccagttactg agcaaaacag gttgtacccg
     1201 gctcacccgc aaagcaaaaa aaacattaat atcatcaaag cgctggaaga tgaacaccca
     1261 catccggaac cattttaaag aaccagctga gcacctgagc aacattaaag gcatcggcgt
     1321 aatgactacg gcggtgctgg tagcctgtat gcgcaaactg ctgaccatcc tgaatgcgat
     1381 actcagaaag aacaaagaaa tgtaatgaat cctgccatca ggttactcca taattttgtt
     1441 ggtcaagaca gttaattacg tgacgtatgc tcaggcaccg gatgccgttt tacagggctc
     1501 acctgccatc cagtagacgc cccggcccac gactgcaacc ataattacgt tgaccagaac
     1561 cgtcatgctg cttcgtctca tacttcacga gataacaggc tctctccatg gctttctgca
     1621 tcgcttccac atcaccacgc ttgacccaga gcacagcgga tccaccagat tttttgtcag
     1681 aagctttccg ggtatcagaa catccacccg acaaaggcgt atcatcggaa gaagggcgag
     1741 catcagatga tgtcggttta cggaaaggcg tccgcctgga aaaatgaacc agtccattac
     1801 cctgccaggg ctcgagatgc agagcactgc accatgccag cttgatcagc gtcgccagcg
     1861 aagaaggaac ggtgaaatcc cctggcgagc accaggtatc tttgttgagc agtaatatca
     1921 cgtgataatg tttcctgcct ttttccttgc caaactcccg acaccagaaa taacgcagag
     1981 tggttgcacg cacacgcttg ttggcacgtc gtttatgtcg ctggtaagcc tgaatttttg
     2041 ctttcaggga tgcgaaaaaa cgggagatca ccgcagagtc cggatcggga tccatgatgg
     2101 tgacaggcat atactcaggg aaacgtaaat ccacccggat taacgaaacg gcacggtgtt
     2161 gcaccagggc actgttgaga atactttcaa aacgggataa ccagtaagca ttgtgtattg
     2221 gttgattcat gatgagagat ctccattgat ttggattgac ttcacatcat taatcaccgc
     2281 ggtaaaactt tacgaactcc caccattgag caacacaacc tactccggat aaaaatggaa
     2341 tcccctacaa acgaaaagat ggtaaaaaaa cacaacaaca gcaaatgaat ttaaaaacgg
     2401 catggacgac gagaaaagtc agaagggaaa cggatcaaca gaacgtgcaa acaaaacatc
     2461 aaaaacgcaa tcagaacgat ttctggcgct gtgaatcaac aacaacacca aaacctatag
     2521 cttaaccaca caactcaatc acagagccga atagcgcgtt taaaacctgt ttacagataa
     2581 ccccacccca agaacaaaaa ccactaacat cagaatcgat tttctcatcc tgaaaatgtt
     2641 acgtttcact acgcaccata aaaacgatta accatcgata acgtaaacct catctgaatc
     2701 aaaacaccag aaccaatcat cacgcttttt ctgaaaagag ccttgccctg agaaaaaaac
     2761 accagggcca gttaatcatc tcatcaatct catcgctaca aaatcatcca taaaaccatt
     2821 taactaaatc gtcgtaagca ctccgtaaat cacgccaggt tattcagtcg gatttgcatt
     2881 tttacgtaaa gatacttttc tccggttaac ggccaaaaca cgacaactca taaaagatta
     2941 aatgtaacat cagtaaaatc gaatagcata ttgactgctt aaacaggaca gggagttttg
     3001 tagtgacgtt aactgatttt agctatcgta aaaacagaac taacaaattt actaaactgg
     3061 atgtgtatca g
//
LOCUS       DAJOTB010000291         2840 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJOTB010000291 DAJOTB010000000
VERSION     DAJOTB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.293"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            395..592
                     /locus_tag="OHN28_004876"
     CDS             395..592
                     /locus_tag="OHN28_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HCQ2300116.1"
                     /translation="MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPH
                     DMVESSAAAWRDEMSRKVAGK"
     gene            589..771
                     /locus_tag="OHN28_004877"
     CDS             589..771
                     /locus_tag="OHN28_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2300117.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVQVHDNIITV"
     gene            870..1178
                     /locus_tag="OHN28_004878"
     CDS             870..1178
                     /locus_tag="OHN28_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300118.1"
                     /translation="MDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGL
                     WGISWGCAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWNDV
                     "
     gene            1738..2772
                     /locus_tag="OHN28_004879"
     CDS             1738..2772
                     /locus_tag="OHN28_004879"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCQ2300119.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNLHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
BASE COUNT          773 a          596 c          703 g          768 t
ORIGIN      
        1 tctgtatatt aaaagagaat ggtaatcatt atcatgttaa agtgtcatat cgtttacata
       61 aagtcaagat atatcagtgt ggcaagcctg gcaggattct ctagggaagg gcaggcatac
      121 tcagtgcata gctgatgcac ggatgacggc aggggggagg aggctggtga ctttcggacg
      181 actgtagcgg cttacatcgg gcttcggcgg tatcatatga aacagcggag tgccgccttc
      241 catgccgctg gcgcggcata tcctagtgac gatatctgaa tcgttatata ctgtgtatat
      301 acgtggtaat gacggggtga taaatggcac aggttaatat gagtttaaga attgacgctg
      361 aactgaagga tgcttttatg gccctgcaaa aagcatggac cgtaatggct ctcagttaat
      421 ccgggatttt atgcgccaga ccgtggaacg gcagcataat acctggttcc gtgaccaggt
      481 tgaggcagga cgtcagcaac tcgagcgcgg cgatgtgctt ccccatgaca tggtcgaatc
      541 ttccgctgct gcatggcggg atgaaatgag caggaaagtc gccggtaaat gatggagata
      601 ttctggacca tgctggccag ccaggacagg aagcgcattc gtgagtacat tgcggagcag
      661 aacctgatag ccgccattga actggacgaa cggattggtt gttcagccag cctcctgttc
      721 tccctttctt ttatcagtgt acaggtgcat gacaacataa tcacggtatg ataataataa
      781 tcaataacaa taagttgtgt cacgtttaca tcatggaacg gaggtgctcc tgtgactttt
      841 gagcaaaaaa agcccgggca attgccctga tggacagcaa aaagatgtgg cggagtaatt
      901 atgctcctcc tctgctgcgt attctctggc ggctgggtat ccggcttccg ccattgccct
      961 ttatgccgtt ctggcaggtt acgctgctga tggggggctt atggggtatt tcctggggat
     1021 gtgcaatgtg gtttatgtac tgggggccgt caggaatggt agcaggtgag gccattatta
     1081 taagtatcac gagtggtttt ttgttcggac tgctcatggc ctctttccac tggtggcgca
     1141 ggaaagttaa ccggctgcca ccatggaatg atgtgtaaac cgtgccggca tgtttttacg
     1201 gggtgatgct ttcattaacc tgtacacgaa cttctattcc tgcatcatta caggcctaca
     1261 gccactacgc cacttccagc ggatcgccct ccctgcgtac ctccccgcct tctttattct
     1321 ataactgcag acaggtgctg ccgtcgaggc gcaccacaaa atccccacgg caggcctgat
     1381 atgtcatgcc gttcagctcc accggcagat cagtggcaga agtgccatct gcgggcagac
     1441 ctgatgccgg gaccagcggc agtacgacga cttctgcagt acgctgttct gccagcagag
     1501 aggctgtgcc acggcccggt cagcttttgt gagttccaca gctagccgca gctcagggat
     1561 taagattcag gtaatttacg gaaaacaagt gatttccgta cgggagagaa ggcctaacgt
     1621 atattatctc agaaatgact atgaatcccc atatattgcc aattatacag taaatgatta
     1681 tcattatcat ctaatgtgtg caactgatgg tctgaaatag cgcttttagg agacaagatg
     1741 cagtcagcta gtaaattaat ctaccgcggg aagttgcttg gcagtttacc cactgtgggc
     1801 gagattcata aagaaatcgc agtatcggaa gaaaccgtaa catggatctc tctgcaacga
     1861 gatattatag cgaatatttt gctgggaaaa gatcctcgct tacttgtgat tgttggtcca
     1921 tgctcaatcc atgacgttca ggccgcagtt gattatgcga agcgattgtt cgtattacag
     1981 aataagtact tatcgaaaat gtacattgta atgcgcactt attttgagaa acctcgaacc
     2041 cgtaaaggct ggaagggaat aatgcacgat cctgatctaa atggaagtta tgatgttgag
     2101 aaagggatac gatatgctcg ccagtgtttg tcttcaatca caacgatgcg tgtcgcaacc
     2161 gctacagaat tcctcgatcc ttttcttact ccctatattg ctgatttaat atgttggggt
     2221 gctattggtg ccagaaccac tgaatcacaa acgcatcgtc aacttgcgtc tggtttacat
     2281 tgtccagttg gatttaaaaa cagtactgat ggaaatatta acttagctat tgacgctatt
     2341 attgcagccc gtgaacaaca tattgtatat atgacatctt taacaaatag tatatctaca
     2401 ctattaaccg atggcaatct acatggacat ctaatattac gtggaggtag agaacctaac
     2461 tatggtttgt ctgatataac taaggcagtg aaattaatgc atgatgaggg cattaaccat
     2521 cgtctgataa ttgattgtag ccatggtaat agcggtaaag tggctaaacg gcaaatttcc
     2581 gttgcacgtc aagttataga taatcggaaa aaaataccag gatatgtagc tggcattatg
     2641 gtggaaagct ttttacaaga tggaaaacaa tccgatagtt ttccgctaga gtatggtcag
     2701 tctgttactg atgaatgcat atcatggcaa cagacagaac aattattatc aactctggca
     2761 gcacagcttt aacttcaact attgcacata tattgctttt aaagggggaa aaatgaaatt
     2821 acttagttta agacactcaa
//
LOCUS       DAJOTB010000292         2748 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJOTB010000292 DAJOTB010000000
VERSION     DAJOTB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.294"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            74..2353
                     /gene="ydeP"
                     /locus_tag="OHN28_004880"
     CDS             74..2353
                     /gene="ydeP"
                     /locus_tag="OHN28_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCQ2300120.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2601..>2748
                     /gene="safA"
                     /locus_tag="OHN28_004881"
     CDS             2601..>2748
                     /gene="safA"
                     /locus_tag="OHN28_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCQ2300121.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMG"
BASE COUNT          738 a          642 c          671 g          697 t
ORIGIN      
        1 taaaccctat gtatatattg tgcatcatcg ctattacaaa tcctaataat tcatttccac
       61 acaggataag cagatgaaga aaaaaattga atcctaccag ggagctgccg gtggttgggg
      121 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat
      181 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggatgtg catggccaga
      241 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      301 agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact
      361 tacctgggga gatcacgagc ttgaggctgc gggacgtctc actcagcctt tgaaatatga
      421 tgacgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
      481 tcgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg ggcgcacttc
      541 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
      601 cgactgctcc aacatgtgcc atgaaccgac cagtgtggga ttggcagcga gtatcggtgt
      661 gggtaaaggg accgtgttgc tggaagactt tgagaaatgc gatttagtca tttgcattgg
      721 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
      781 gggagcgaaa atgatcgcta tcaaccctct acaggaacgt ggcctggagc gatttaccgc
      841 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttagcca gtgcctacta
      901 taacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
      961 gcgcgatgat gctgcaagcg ccgcaggtcg gccctcattg ctggatgacg aatttattca
     1021 aacgcatacc gtcggctttg acgagctacg ccgtgacgtg ctcaattccg agtggaaaga
     1081 tatcgaacgt atttctggac taagtcagac acaaatcgcc gaacttgctg atgcatatgc
     1141 cgctgccgaa cgaaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     1201 ccagaacgta cagcaactgg tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg
     1261 tgcgggtatc tgcccactac gaggtcactc taatgtacag ggcgaccgaa ccgtcggtat
     1321 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcacccc
     1381 acctcatgca cctggacatg ctgcaatagc cagcatgcaa gctatatgta cggggcaagc
     1441 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     1501 cgctgtaccg ttaacgcaat tagatctggc ggtacacgta gccactaagc ttaaccgctc
     1561 tcatctgctg accgcacggc atagctatat tctgccggtt ctgggacgta gcgagattga
     1621 catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tggttcatgc
     1681 ctcgcgtggt gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc
     1741 gggaatcgcg caggcagcac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     1801 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagtttgccg actataacca
     1861 gcgcatccgt catcctggtg gttttcacct gataaacgca gctgctgaaa ggcgctggat
     1921 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccttcttc
     1981 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     2041 gatttatggt atggatgatc gctatcgagg tgtattcggt caacgagatg tggtctttat
     2101 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     2161 actcacgcca gacggtaaac gtagctcacg ccgcatggat agattaaaag tggtcattta
     2221 ccctatggct gaccgctcac tggtgaccta tttcccagaa tcgaatcaca tgctaacact
     2281 tgataaccac gatccattaa gtggcattcc tggctataaa agtattccgg ttgaattaga
     2341 accatcaaat taattctcct cctcatttct tctgctgtca tccgcacagc agaagaattc
     2401 ctcattgact attatcgcgc aatttgctca catggattaa attaaactac acactataag
     2461 atataaacct ctgcctacag ttgtaagaaa ctccgctcag tactgaagtg ccagtcctat
     2521 ttcctctttt atccagcctg ttatattaat catactgatt aacgattttt aacgttatcc
     2581 gctaaataaa cacatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag
     2641 acaactataa agaaatcatg tctgtaattg tggttgtctt gttactgaca cttacgttga
     2701 tagccatttt ttcggcaatt gatcagctgg gcatttcaga aatgggtc
//
LOCUS       DAJOTB010000293         2668 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJOTB010000293 DAJOTB010000000
VERSION     DAJOTB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.295"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1571
                     /locus_tag="OHN28_004882"
     CDS             <1..1571
                     /locus_tag="OHN28_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCQ2300122.1"
                     /translation="KAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPF
                     LAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMH
                     LGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLR
                     IPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFY
                     AVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLA
                     GKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFL
                     FVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEV
                     AYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMV
                     KALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVIIGTKV
                     IALRRDMDNYMGIY"
     gene            1571..>2668
                     /locus_tag="OHN28_004883"
     CDS             1571..>2668
                     /locus_tag="OHN28_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2300123.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGSYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IGYNECEGWLKSLKALKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGY
                     PFDEFYSRLIHEGKVGIMAGHVYGTEGEGYLR"
BASE COUNT          725 a          494 c          616 g          833 t
ORIGIN      
        1 agaaagcctg gagttttttt cagagcctgg ggaaggcatt tatgtatccc attgctctgc
       61 taagtgtatg tggcatgatg ctagggctgg gaagtggttt agccagtgat gatatggcaa
      121 agttaattcc atttctggct atcccaataa ttaaaaccat acttgatttc attgttagtc
      181 ttggtttgtt tgcctttgtt aatttacctg tattgtttgc gatagcgatt cccttaggat
      241 tattaaaaga taaagaggat aaagcctatg gtgctttttc tggcttaatt ggttttatgg
      301 cgatgcatct gggaacgaac ttttatctta aacagcacga cttattggtc gttgctgacc
      361 aaatgtcgac acatgggcaa accatcattc tggggatcca gtcctacaat accagcgtgt
      421 tggggggaat tgttgctggg ttattagtcg ccagcatgta taaaaagatc gttaatttac
      481 gcattcctga atcgttaggt ttttatagcg gcccacgtct ggtgcctatc attacactga
      541 ttgtgatgag tggatttggt ctgatcattc cttttatctg gccgccgttt ttcaatcttt
      601 tcatgctcat tggacactgg atttcaactt ccggtcctgt tggttatttc ttctatgcag
      661 ttgccgaacg cgtgacgatt ccttttggct taaaccatct ggtgacgtca gttttccgct
      721 ttacgccaat cggcggttcg gctgtgattg gcggcgaaga atattacggc accctgaaca
      781 tgtttatggc gtacgtcaaa gagaatgcgg tcattccgct ggatttggcg gggaaaatgg
      841 agcagggcaa actgatgatt cagtatggtc tggctggtgc cgcgctggcg atgtatcgca
      901 ctgctcatgc tcaaaacaga aaggctatca aagcattgct tatttccggg gtgcttacgg
      961 tgattattgg cggcgtcagc gaaccgattg agtttctgtt cttatttgtc agtccactgc
     1021 tgtttgtctt ccatgccttt atgaatggat tcgctaacat ggttctgcca tatatgggcg
     1081 tgaagatggg atttactggc gatctgattc aatttattag ctttggcgta ttgcgtggca
     1141 caagaacagg ttggccgatc gcggtgtgtg tcgaagtggc ctatttcttc atttattact
     1201 ttgtgttccg ttggaccatt cttaaattta acctgatgac cgtaggccgt gaagagtcca
     1261 gtcctgtcac gctgaacgct cacgaagata cggctatagc ggatatccca actcctgata
     1321 aatcagagct gcaagcggcg gagcagatgg ttaaggcact tggtggtaaa gagaatatta
     1381 agtcactgga taattgcgta actcgtttac gtttaacaat cgcagatatg ggattgattg
     1441 acgaagttgc aataaaaata gctggcggga ttgcggttgt taaacttgat caaaataccc
     1501 tacaagtcat tatcggcact aaagtcatcg ccctgcgtcg ggatatggat aactatatgg
     1561 ggatatactg atggatgttt ttaatactcc tgtttcccgc aaaggaacat attgtaccca
     1621 atgggacttt tgcgaggaca gatttggtgt aaaagatgta ctaccattct ctatttcaga
     1681 tatggatctt ccgataccag acgctatcac cagagcatta aaaaaacgcc tggaacaccc
     1741 gattttaggg tacagtcgtt ggcaacatgg tgaatatctg aatgcgatag taaactggta
     1801 ttatcaacaa tatcaaacag atattaaacc tgaatggata acctatagcc ccagtgtgat
     1861 gtactctatt gcaaaagcaa tagagttatt gacatcgcat ggtgataata ttctcgtttt
     1921 tacacctgtc tataatgcat tttttgatgt aattaaacat agcgagcgaa atattctgac
     1981 agcctctttg attataaaaa atgaggggag ctacgctatc aactggcaag attttgatct
     2041 taagattaaa agcgcaaaga tggtgttatt atgtaatcct cataacccga caggaacagt
     2101 ctggtcggaa gaagaactgc ataaaatcgc tgcaagctgt acaaggcata atgtctggtt
     2161 atgttcagat gaaatccata gtgattttgt ttttaatcgt agctttacat ccgcgcttaa
     2221 aataaaaaaa gaaaaagtag tagtatttaa ctcgatatcg aaaacattta atgttcctgc
     2281 actaacagga tcgtatatga tttcaactga cgataatttt aatcataagt tcagaacgat
     2341 atcgaggtat cgcgattttg taaactcccc atcagtactg aatataattg caactattat
     2401 tggctataac gaatgtgagg gatggttaaa gtcattaaag gcattaaagg cacatattgc
     2461 ctctaacatt caattcaccc agcagtatct gaatgaaaat attccagaat tgatcgtaag
     2521 gccagctgat ggttgctatt tttcctggat agattgttct gctattggtt atccttttga
     2581 tgaattctac agtcgattaa ttcatgaagg caaagtgggc attatggcgg ggcatgtcta
     2641 tggaacggag ggggagggct accttcgt
//
LOCUS       DAJOTB010000294         2613 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJOTB010000294 DAJOTB010000000
VERSION     DAJOTB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.296"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2455
                     /locus_tag="OHN28_004884"
     CDS             <1..2455
                     /locus_tag="OHN28_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HCQ2300124.1"
                     /translation="TCLTELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGTG
                     GQYDFDDVIQNENTFLSLLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAIIQ
                     KKLNNDVSRLEENIKKNRCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGES
                     ITDNAANIDFIYFLLSHGYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETAK
                     MPLSNIANTVAKLHGLGILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRMV
                     RLANEIFPLWEPAAQREYIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPIL
                     SWEAVSQITREELQRLIDLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSGQ
                     ETLHLVVQKKLWTYSTLNLQNICFSLSHESENNSDTFRKKPVALIKSLRIPNLEKYVY
                     ENISSFIRDVFIHSEENDLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENTE
                     TTEISYDQNLYSLLAHHNHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDSL
                     PLTDVQFSQLLIKAVTSPHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWLA
                     PTSTVFEQLYQALYEEGDKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLIL
                     NGDKIADEVCISILNWLWEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCLK
                     NDGGSHKFIRQVLMTFGHQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLT
                     HADTRIRIEPFFNAENEYD"
     gene            complement(2500..>2613)
                     /locus_tag="OHN28_004885"
     CDS             complement(2500..>2613)
                     /locus_tag="OHN28_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HCQ2300125.1"
                     /translation="NDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
BASE COUNT          777 a          549 c          545 g          742 t
ORIGIN      
        1 aacgtgtcta actgaactat ctaaactaca taatgaagaa gtggtcactg aacatgagtt
       61 aagaagtgaa attttgcggc catatatcag tgaaaaaaca gcaccccgat tacatattat
      121 gacaggtaca ggagggcaat atgattttga tgatgtaatc caaaatgaaa atacatttct
      181 ttctttgctg agcaaccaaa taatcagcat taaacctagt ggatataaca ttactataac
      241 aacaatcgat aaaaaaacag ctcaggttat gaaggaggaa tatcaggaaa gaatagcgat
      301 tattcaaaag aaactaaata atgatgtttc tcgattggag gaaaatatta aaaaaaacag
      361 gtgcgagatt cagaatgcat cctcgtatga tcttgctttt ttcatcaata aaatggggag
      421 atcaggtttc gaaagatata ttgcatgttg ttcaacgcct gaacaacatg acggagaatc
      481 cattactgat aatgccgcaa atattgattt tatttatttc cttctatctc atggttatct
      541 ttccactgac tatatggcct accgctcagt ctttatgcca ggtagtctga gcacggaaga
      601 caataacttt atcagggctg tgacgtcagg aagattacct gatgaaacgg caaaaatgcc
      661 cctcagcaat attgctaaca cagtagcaaa attgcatgga ctgggcatac tgatgcatga
      721 caatgcatgg catcctcaga ttctctggta cctcatgcgc aatgatacga actctctgaa
      781 gactatcatg aggatgcagg cagaggtcgg agccgaacgg cggatggtaa gactggccaa
      841 tgagatcttc cccctatggg aacctgctgc gcagcgggaa tatatcagat taatggtcga
      901 cggggatggt catttatcca ctatgatcca ccagattggg cgcctgaatg ataccgtagc
      961 tgaacaaaat cttctcccgg tattactgtc cttaccgata ctttcttggg aagccgtctc
     1021 tcagataact cgggaagaac tgcagagact catcgatttg caatttaatc tcgtaacgtc
     1081 cttaccggag aattgtgcgc agtttttctg tgaaaacctg cggaattcag gttgtcgcct
     1141 gactaacatt cctttggccc ggagtgactc tgggcaggaa acattgcatt tggtggtaca
     1201 gaaaaaattg tggacctatt ccacgttaaa cctgcaaaac atttgtttct cactttctca
     1261 tgagtcagag aataacagtg ataccttccg taagaagcct gttgctttaa taaaatcgct
     1321 tcgtattccg aaccttgaaa aatatgttta tgaaaacata tcatcattca ttcgtgatgt
     1381 ttttatacac tctgaagaaa atgatctcat tcctgatttt cttaattcga cattcgttga
     1441 ctgggatgat gcaaagtaca tgacagaaag tatgagcttc gtactggaag acgtatcagt
     1501 tatcctgaac aaagagaata ccgaaacaac agaaatttct tatgatcaaa acctgtattc
     1561 acttctggct catcataatc atattacccc ttgctggaat aatgttattt ctctactgag
     1621 tgaagatgcg tcaattgctg gtgatacttt ttgtgagtgg cttaatatta attattcttt
     1681 attgccaaat gactcgttgc ctttgactga tgttcagttc tcgcaattac ttattaaggc
     1741 tgttacatca cctcatatca gtaaagaagc gttaatagcg ataaccatgg catttaggat
     1801 aactcttatt aatgtgccag aaaacttacc actgaataat gctgcggtac taatcaagca
     1861 aaaatggctg gcaccaacca gtactgtgtt tgaacaactt tatcaggcac tgtacgaaga
     1921 aggtgacaaa ctgacatcac tactttatgc acttatttgt gcccgtcctg tattattgag
     1981 tgataattac gagcttgttt tattttctga tgaccagttt gacctgggga ttactcgttt
     2041 aattctgaat ggcgataaga ttgcagatga agtctgtatc agtatcctga actggctctg
     2101 ggaaaaagat gaagccctgc tctctgaggc accacttctc agccagcaag ccctgataag
     2161 gttctccacc aaaataaccg atgaccggca gaagcaggca ctgctgatgc aatgtcttaa
     2221 aaatgacggt gggtcacaca agttcatcag acaggtgctc atgacgttcg ggcatcagga
     2281 ttatgccgct ttcctcacag agaggaatta tcgtagcatt ccccgtagtg atgccatgtg
     2341 gcaactggct gtccagctgg gaaatagtgg atttatcagg ccgccgaaac ttactcacgc
     2401 tgatacacgt attcgtatag agcccttttt taacgctgaa aatgaatacg actgacatcg
     2461 caaaatagtg gtaagtccgt cgggggcggc cttactcctt tatcctgccc agtcttccag
     2521 taccagtcca ggcacccgct caaactctct cacattattc gtcaccagta ttgccccggt
     2581 ggcgatggcg tgcccggcga tcgccgtgtc gtt
//
LOCUS       DAJOTB010000295         2597 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJOTB010000295 DAJOTB010000000
VERSION     DAJOTB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.297"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            212..1747
                     /gene="istA"
                     /locus_tag="OHN28_004886"
     CDS             212..1747
                     /gene="istA"
                     /locus_tag="OHN28_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCQ2300126.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTFASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GNKAMGYSHFCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRILDAPALSPLPLAPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKAGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRQHGTWTPERLIEQGKRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRENLELPLSRQGEQHPAYASEHENLRGPG
                     YYH"
     gene            1764..2519
                     /gene="istB"
                     /locus_tag="OHN28_004887"
     CDS             1764..2519
                     /gene="istB"
                     /locus_tag="OHN28_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCQ2300127.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNL
                     LITGPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPVVESSEKTR"
BASE COUNT          700 a          685 c          684 g          528 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatgcttc cgatcattga ttggcatcct gccagtcact
      121 ccgggaatta actcttttca ccacagtctc cattgccgtg tttaaaccag tggagacgtc
      181 aatgtccaga aagagaaaat ccaggagcac tatggaaacc tgttttaaga tccttcagct
      241 caagttcgac aagaagctga ctaaccgttg tatcggactg acgttacaca tcagcgcctc
      301 aaccgttttt gaggttcttg cccgcttcaa agcctcttct ctgtcatggc cattacctgc
      361 tgatatatcc catgacacgc tggagaaact catttttccg cccaaagaca cctttgcatc
      421 agagcttgta atgcctgaca tgttgtactt cgatacggaa atgagaaaac caggtgtgac
      481 gcggcaactg ttgtggatgg aatacaaggc acaggcgggt aacaaagcga tgggatactc
      541 gcacttctgc cgttgctacc gggagtggaa aaaaacgcga cgtctctcca tgcgccagga
      601 gcatcgtgcc ggcgaaaagc tttttatcga cttctgtggt cctacggttc ctgttattaa
      661 tccggacacc ggtgagatac gccgcgtggc aatcttcgtg gccgtaatgg gggcgtccaa
      721 ctatacctac gttgaagcgt gcgaaggtca ggacatgatg tcatggttaa atgcccacag
      781 ccgttgcctg accttcctgg gcggcgtacc gaagctgctc atccccgata acctcagaag
      841 cgcagtgaaa aaggccgatc gatatgagcc tgtcatcaac gacagttatc aggcactggc
      901 tgagcactat ggcacggtta tcatcccggc ccgaccacgc aaacctaaag ataaaccaaa
      961 agcagaaaac ggcgtcctca tcgttgaacg atggcttctt gcccgcatcc gtaatgaaac
     1021 cttccacacg ctcagggcgc tgaatgcgcg ccttagggag ttactgacag acatgaataa
     1081 ccggccaatg aaggggtacg gcaatcaaac ccgtgcagaa cgcttccgca tactggatgc
     1141 gccagcactc tctccgctgc ctctggcacc ttatgaatac acggaataca aagctgtgaa
     1201 ggttggccct gactaccacg ttgaatatgc ccgtcactgg tactccgttc cacacgaact
     1261 ggtcggtcag cgtctgtcac tgaaggcggg gcagtcggtg gtacagctct ggcacaaggg
     1321 gcagtgtgtg gcgcaacatc cgcgcagcac acatgaatat aaacacacaa ccaatccgct
     1381 tcacatgcca gagcgccacc ggcagcacgg gacatggaca ccggaacgcc tgatcgagca
     1441 gggaaaacgt acgggcccgt ccacaggccg ggtggttgaa tccatgctga aggcaaaacc
     1501 ccatccggaa ctggcttatc gtgcagttct ggggctgctg gcacttcaga aaaaatacgg
     1561 cccggaacgg ctggaaaagg cctgttacgt tgccctgcac tataacgccc ctgacagacg
     1621 ctttatcgac aatctgctgc gtcaccatcg ggagaacctg gagcttccat tatcccgcca
     1681 gggcgaacaa catccggcat atgcttcaga gcatgaaaac ctgcgtggtc cgggctacta
     1741 ccactgacag aaggaacaac agtatgagtg ataacctgtt aaacaaactg acccaactga
     1801 aactcccggc gatggccggg agcctgatac ggcagcggga aacgcctcag acctacgacg
     1861 aactgtcgtt cgaggaaagg ctgacgctgc tggttgatga cgagcttctg agcagggaaa
     1921 acagccgtgt ggcacggttg agaaaaaatg ccggcctgaa ataccaggca acaccggaag
     1981 ggctgcgcta tccggcctca agaggtctgc gggctgaaca gatgagagaa ctgctgaacg
     2041 gccactacat aacccacagg aaaaacctgc tgataaccgg tccgacagga tgtggcaaga
     2101 gctggatagc caatgcgctg ggcgaacagg catgtcgaca gaaacacagc gtccggtact
     2161 gtagaaccgg gcgattactg gaacaactgg ctcagggacg tgttgatggc agctggctga
     2221 aatatctgaa acagttgcaa aaaatacagg ttctaatcct ggacgatctg gggctggaac
     2281 agttaagtaa cgcccagtgt aatgaccttc tggagctcat agaggatcgt tacggacaaa
     2341 gcagcacgat agtggtaagc cagttcccgg tagataaatg gcatggtctg atggagaacc
     2401 caacaacagc agatgccatc ctggacagac tggtacataa ctcacaccgg gtggtccttc
     2461 agggagagtc actgaggaaa aatcccccgg tagtggaaag cagcgaaaaa acgcgttaaa
     2521 aagcggtaga aaaagttaag ttcccggttg atcgaatgga tgcgaatcgc tgatcggact
     2581 tcgatgccaa tccgtga
//
LOCUS       DAJOTB010000296         2571 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJOTB010000296 DAJOTB010000000
VERSION     DAJOTB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.299"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            117..728
                     /gene="ruvA"
                     /locus_tag="OHN28_004888"
     CDS             117..728
                     /gene="ruvA"
                     /locus_tag="OHN28_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCQ2300128.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            737..1747
                     /gene="ruvB"
                     /locus_tag="OHN28_004889"
     CDS             737..1747
                     /gene="ruvB"
                     /locus_tag="OHN28_004889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCQ2300129.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
     gene            1857..2571
                     /locus_tag="OHN28_004890"
                     /pseudo
     CDS             1857..2571
                     /locus_tag="OHN28_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          635 a          616 c          692 g          628 t
ORIGIN      
        1 gccatttttc agatcatcga gacacctcgc aagttttctt catccttcgc tggatatcta
       61 tccagcattt ttttatcata cagcattatc tttgattcat tacgcaggag cgtcatgtga
      121 taggcagact cagaggcatc atcattgaaa aacaaccccc gctggtgtta attgaagtgg
      181 gcggcgtagg ctatgaagtg catatgccga tgacctgttt ttatgaactc cctgaagcgg
      241 gtcaggaagc gatcgttttc acccactttg tggtgcgtga agacgcgcaa ctgctgtacg
      301 gttttaacaa taaacaagag cgcacgttgt tcaaagagtt gatcaaaacc aacggcgtcg
      361 gcccgaagtt ggcgctggcg atcctctccg gaatgtcagc gcagcagttc gttaatgccg
      421 ttgagcgtga agaagtgggg gcactggtga aactgccggg tattggcaaa aaaaccgccg
      481 aacgtttgat tgtcgaaatg aaagaccgat ttaaaggttt gcatggcgat ctctttacgc
      541 cagccgccga cctggtactc acgtctcctg ccagcccggc gaccgacgat gctgaacagg
      601 aagcggttgc tgcgctggtg gcactgggct ataaaccaca agaagcaagc cgcatggtga
      661 gcaaaatcgc tcgccctgac gccagcagtg aaacattaat tcgcgaagcc ctacgcgccg
      721 cgttatgagg taaaggatga ttgaagcaga ccgtctgatt tctgccggta ccactttgcc
      781 ggaagatgtg gcagatcgcg ccattcgccc taaattactg gaagagtatg ttggtcagcc
      841 gcaggttcgt tcgcagatgg agattttcat caaagcagcg aaactgcgcg gcgatgccct
      901 cgatcatcta ttgatttttg gtcctccggg gttgggtaaa actacgcttg ccaatattgt
      961 cgccaatgaa atgggcgtta atttacgcac gacttctggt ccggtgctgg aaaaggcggg
     1021 cgatctggca gcaatgctca ctaaccttga accacacgat gtactgttta ttgatgagat
     1081 ccaccgtctt tcaccagtgg tggaagaggt gttgtatccg gcaatggaag attaccaact
     1141 ggatatcatg attggtgaag gtccggcggc acgctccatt aaaattgatt tgccgccgtt
     1201 taccctgatt ggtgcaacca cgcgcgcagg ttcgctgaca tcaccgttgc gcgatcgttt
     1261 tggtattgtg caacgtctgg agttttatca ggtgccggat ctgcaatata tcgtcagtcg
     1321 cagcgcacgc tttatggggc ttgagatgag tgatgacggc gcgctggaag ttgctcgtcg
     1381 tgcgcgcggt acgccacgta ttgccaaccg tctgctgcgt cgagtgcgtg atttcgccga
     1441 agtgaagcac gatggcacca tctcggcaga tatcgctgct caggcgctgg atatgttgaa
     1501 tgtcgatgct gaaggtttcg attatatgga ccgcaaattg ttgctggcgg taatcgataa
     1561 gttctttggc gggccggtag gtctggataa cctggcggca gccattggcg aagaacgtga
     1621 aaccattgag gatgtactgg aaccttattt gattcagcaa ggctttttgc agcgtacacc
     1681 gcgtgggcgt atggcgacga cgcgggcgtg gaatcacttt ggcataacgc cgccagaaat
     1741 gtcgtaagtc ggattggtcg ctcgtgccgc atccgacact ggcagtgtgc ctgatgcgac
     1801 gctgttgaac tgcaccccaa atgttggaca taatctgaca tctgaaggtg cagtttatga
     1861 aacattcatt tgaagtaaaa cttgccgctg taaatcatta cctggctggt catgcaggca
     1921 tcatttctac ggcaaaactc tttcaacttt ctcacaccag cctctcacac tggattaatc
     1981 tttttcttct tcacggtcct cgggcactgg attgcagaca caagcgtagc tattctcctg
     2041 aagataaact ttgcgtggtt ctttatgctc tcgggcattc tgagtctcta ccccgggtag
     2101 cagcccggtt taacattccc agccacaaca cggttaaaaa ctggataaaa ggctaccgca
     2161 aatctggtaa tgaggccttt atcaggcgca ggaaggagaa aagcatgacc cgttctgatg
     2221 atacccatga aaacgaggca aacatgacgc cggaagagat gaagaatgag ctgcgttatc
     2281 ttcgcgcaga aaatgcatat ctgaaagcca tgcaggagca tcttctggaa aaaaagcgcc
     2341 aggagctgga gaaaaaacga aagtcatcca gagcctgagg tacggacact gccagtctga
     2401 cctgttaaag gccgcagggc tggccagaag cacgctgtac taccagctgt cactgcagaa
     2461 ggctaaggac aagtatgctg atgtgaaaca gctcatagcc tccatcttcc atgaacaccg
     2521 gggatgttat gggtaccggc ggatccattg tgagcttcag aaaagaggtc t
//
LOCUS       DAJOTB010000297         2556 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJOTB010000297 DAJOTB010000000
VERSION     DAJOTB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.300"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2526
                     /locus_tag="OHN28_004891"
     rRNA            <1..2526
                     /locus_tag="OHN28_004891"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          663 a          557 c          806 g          530 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctcttaat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      601 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      661 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      721 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
      781 gcggcagcga cactatgtgt tgttgggtag gggagcgttc tgtaagcctg tgaaggtgta
      841 ctgtgaggta tgctggaggt atcagaagtg cgaatgctga cataagtaac gataaagcgg
      901 gtgaaaagcc cgctcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt
      961 gagtcgaccc ctaaggcgag gccgaaaggc gtagtcgatg ggaaacaggt taatattcct
     1021 gtacttggtg ttactgcgaa ggggggacgg agaaggctat gttggccggg cgacggttgt
     1081 cccggtttaa gcgtgtaggc tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt
     1141 gatgacgagg cactacggtg ctgaagcgac aaatgccctg cttccaggaa aagcctctaa
     1201 gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta gagaatacca
     1261 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga
     1321 aggcacgctg atatgtaggt gaagcgactt gctcgtggag ctgaaatcag tcgaagatac
     1381 cagctggctg caactgttta ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat
     1441 acggtgtgac gcctgcccgg tgccggaagg ttaattgatg gggttagcgg taacgcgaag
     1501 ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
     1561 ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag gctgtctcca
     1621 cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg gcaagacgga
     1681 aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga tgtgtaggat
     1741 aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg aaataccacc
     1801 ctttaatgtt tgatgttcta acgtgggccc cttaccgggg ttgcggacag tgtctggtgg
     1861 gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa
     1921 tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact gcgagcgtga
     1981 cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca
     2041 tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata
     2101 tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc
     2161 ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag aacgtcgtga
     2221 gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg ctcctagtac
     2281 gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg gcactgcccg
     2341 gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgccccgaga
     2401 tgagttctcc ctgactcctt gagagtcctg aaggaacgtt gaagacgacg acgttgatag
     2461 gccgggtgtg taagcgcagc gatgcgttga gctaaccggt actaatgaac cgtgaggctt
     2521 aaccttacaa cgccgaagat gttttggcgg aagaga
//
LOCUS       DAJOTB010000298         2439 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJOTB010000298 DAJOTB010000000
VERSION     DAJOTB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.301"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            286..429
                     /locus_tag="OHN28_004892"
     CDS             286..429
                     /locus_tag="OHN28_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCQ2300130.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            442..651
                     /gene="cspE"
                     /locus_tag="OHN28_004893"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             442..651
                     /gene="cspE"
                     /locus_tag="OHN28_004893"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2300131.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            817..1626
                     /gene="rlmA"
                     /locus_tag="OHN28_004894"
     CDS             817..1626
                     /gene="rlmA"
                     /locus_tag="OHN28_004894"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCQ2300132.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLREWLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLLFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1623..2189)
                     /gene="mntP"
                     /locus_tag="OHN28_004895"
     CDS             complement(1623..2189)
                     /gene="mntP"
                     /locus_tag="OHN28_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCQ2300133.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          648 a          576 c          593 g          622 t
ORIGIN      
        1 gaaaatgtga tattgcacgc actatgtaat aacttctccc actggcctgg aacaactgaa
       61 cttattgaac tatgttagaa aatacgtcag tttaagtatc tgcctgaact ggcaaggtta
      121 agcacaatga tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa gtggaagacg
      181 tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat aattaacgtt
      241 gtatgtgata acagatttcg ggttaaacga ggtacagttc tgtttatgtg tggcattttc
      301 agtaaagaag tcctgagtaa acacgttgac gttgaatacc gcttctctgc cgagccttat
      361 attggtgcct catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa
      421 acaatctaag gaatttttca aatggcaaag attaaaggtc aggttaagtg gttcaacgag
      481 tctaaaggtt ttggcttcat tactccggct gatggcagca aagatgtgtt cgtacacttc
      541 tccgctatcc agggtaatgg tttcaaaact ctggctgaag gtcagaacgt tgagttcgaa
      601 attcaggacg gtcagaaagg tccggcagct gttaacgtaa cagctatctg atcgaatcca
      661 ctgatctgaa gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt
      721 tttactttgt tttatcttcg ctccagtcgt tcggatattt gcccgccgca tgattcgcgt
      781 tacacttgcg gcctttagta tcctgccgga gttgtcatgt cgttttcctg tccactttgc
      841 catcagcctc ttttgcgtga aaaaaacagc tatatctgtc cccagcgaca tcagtttgat
      901 atggcgaaag aagggtatgt caatctgctg cccgttcagc ataaacggtc tcgtgatccg
      961 ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct tagatgccgg acattatcag
     1021 ccgctgcgtg atgcaattgt cgcccaactg cgggaatggc ttgatgaaaa ggccacggcg
     1081 gtgctggata ttggctgtgg tgaagggtat tacacacacg catttgctga tgcgttgccc
     1141 gaaatcacca cgtttggcct ggatgtctcg aaggtagcga taaaagcggc ggcgaaacgc
     1201 tatccgcagg tcactttttg tgtcgcttcc agccaccgtt tgctgttttc cgataccagt
     1261 atggacgcca taatacgtat ttacgcgccg tgtaaagcag aagaattagt gcgagtagtg
     1321 aagcccggcg gttgggtcat tactgccacg ccgggaccga gacacttaat ggagctgaag
     1381 gggctgattt acaatgaagt acatcttcat gcacctcatg cagaacaact gaaaggtttt
     1441 acattacagc agagtgatga gttgtgttat ctgatgcgtc ttcgcggtga tgaagccgtc
     1501 gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc ctgaagtctg gcaagcactg
     1561 gcagcaaaag aagtgttcga ctgccagacg gactttaata ttcacctctg gcagcgttct
     1621 tattaaccgt ggaagtgcgt ccagaggatc tggacgccaa taccgatcag taccagccca
     1681 ccgagaattt ccgctttttt cccaataatt gagccgataa agcgaccaac catcatccct
     1741 aatgtcgaca taatcaaggt tgcacaacca atggccaatg cggtcgcgat aatgttaacc
     1801 tgcaggaaag caagaccaac acccacagcc atggcatcca ggctggtggc aatcgcggtg
     1861 gttaccagta gccagaaacc gtgtcgacgg cgcggttctt catcttcatc atctgcgcca
     1921 cgaaaaccct caataatcat tcgcccgccg agaaatatca gcagcacaaa cgcaatccag
     1981 tggttccatt caaggacaaa ccgactggct aacatgccca ttccccagcc gatcagcggc
     2041 gtcagggttt cgacggcacc aaaaataagg ccggttcgca atgcttcaga aaatttcggt
     2101 ttatggaggg tggcaccttt accgattgat gcagcaaagg catccatcga cataccaaac
     2161 gcaagaagaa cagtagcagt gatattcatg acaacgtcct gaccggggta tccataaaac
     2221 acatcatcgc ccccagcaaa catgcataca tgaacaacaa gccgcgatgg ttttcgcgtt
     2281 catgcaggca tagcgattga tgtgcatatg gtctcgcctg actgaaagtg ttcagtccgt
     2341 acgtgccacg ttttgcaacg agtatgttga cacgtacatt tccggaatct ggaaatcagc
     2401 tactccccaa atgagggcgc aaccttaaca tattttgag
//
LOCUS       DAJOTB010000299         2425 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJOTB010000299 DAJOTB010000000
VERSION     DAJOTB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.302"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..130)
                     /locus_tag="OHN28_004896"
     CDS             complement(<1..130)
                     /locus_tag="OHN28_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300134.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAG"
     gene            446..1498
                     /gene="aroG"
                     /locus_tag="OHN28_004897"
     CDS             446..1498
                     /gene="aroG"
                     /locus_tag="OHN28_004897"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCQ2300135.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(1665..2417)
                     /gene="gpmA"
                     /locus_tag="OHN28_004898"
     CDS             complement(1665..2417)
                     /gene="gpmA"
                     /locus_tag="OHN28_004898"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2300136.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
BASE COUNT          601 a          601 c          611 g          612 t
ORIGIN      
        1 ggcccgcatc agcggcggcg tttgcctggc cgttattagt ttgcgctccg ctatcggcag
       61 ccagtgcggc accgctggca aggcttagag tggcagtcaa aaatagtgtg gccagttttg
      121 tcattttcat aggatgctcc tgttatggtc gttatgtcgg ataacctctt ccaacagtgc
      181 atttgcaggt gaatataagg caatggttta agatttcagc caggcaaaga tacccagcag
      241 agaatcttga aataattaac aaacaaagga gttacagtta gaaattgtag gagagatctc
      301 gtttttcgcg acaatctggc gtttttcttg ctaattccag gattaatccg ttcatagtgt
      361 aaaaccccgt ttacacattc tgacggaaga tatagattgg aagtattgca ttcactaaga
      421 taagtatggc aacactggaa cagacatgaa ttatcagaac gacgatttac gcatcaaaga
      481 aatcaaagag ttacttcctc ctgtcgcatt gctggaaaaa ttccccgcta ctgaaaatgc
      541 cgcgaatacg gtcgcccatg cccgaaaagc gatccataag atcctgaaag gtaatgatga
      601 tcgcctgttg gtggtgattg gcccatgctc aattcatgat cctgtcgcgg ctaaagagta
      661 tgccactcgc ttgctggcgc tgcgtgaaga gctgaaagat gagctggaaa tcgtaatgcg
      721 cgtctatttt gaaaagccgc gtaccacggt gggctggaaa gggctgatta acgatccgca
      781 tatggataac agcttccaga tcaacgacgg tctgcgtatt gcccgtaaat tgttgcttga
      841 tattaacgac agcggtctgc cagcggcggg tgagtttctc gatatgatca ccccacaata
      901 tctcgctgac ctgatgagct ggggcgcaat tggcgcacgt actacggaat cgcaggtgca
      961 ccgcgaactg gcttcagggc tttcttgccc ggtaggcttc aaaaatggca ctgatggtac
     1021 gattaaagtg gctatcgatg ccattaatgc cgccggtgcg ccgcactgct tcctttcagt
     1081 gaccaaatgg ggtcattcgg cgattgtgaa taccagcggt aacggcgatt gccatatcat
     1141 tctgcgcggc ggtaaagagc ctaactacag cgcgaagcac gttgctgaag tgaaagaagg
     1201 gctgaacaaa gcaggcctgc cagcacaggt gatgatcgat ttcagccatg ctaactcgtc
     1261 taaacaattc aaaaagcaga tggatgtttg tgctgacgtt tgccagcaga ttgccggtgg
     1321 cgaaaaggcc attattggcg tgatggtgga aagccatctg gtggaaggca atcagagcct
     1381 cgagagcggg gaaccgctgg cctacggtaa gagcatcacc gatgcctgca ttggctggga
     1441 tgataccgat gctctgttac gtcaactggc gaatgcagta aaagcgcgtc gcgggtaaag
     1501 tttaattgtc ggatgcgctg tcagagtggc gtatccgatt aatcaccgta ggcctgataa
     1561 aacgcgcagc gtcgcatcag gcaatgcgct ccattggtag caacgaaaaa taaaaaagcc
     1621 gactcatttt gcagtcggct ttctcatttt taagcgaatg acgtttactt cgctttaccc
     1681 tggttagcaa ccgccgctgc tttcgccgcg atctcgtcag cattacccag ataatagcgt
     1741 ttcagcggtt tgaagttctc gtcaaactca taaaccagcg gcacgccggt cgggatattc
     1801 agctcaagaa tctcttcttc gctcatgtta tcgagatatt tcaccagcgc acgtaacgag
     1861 ttaccgtgtg cggcgatgat gacgcgctca ccgctcttca tacgcggcag aatggtttca
     1921 ttccagtaag ggatcacgcg gtcaatggtc agcgccaggc tttccgtcag tggcagctct
     1981 ttctcgctca gtttcgcgta acgcggatcg tgacccggat aacgctcatc atctttagtc
     2041 agttccggcg gagtcaccgc aaaaccacga cgccactgtt tcacctgctc gtcgccatac
     2101 ttttcagcgg tttccgcttt gttcagaccc tgcaatgcac cgtagtgacg ttcgttcagt
     2161 ttccaggatt tctcaacggg cagccatgcc tgatccagtt cgtccagcac attccacagg
     2221 gtatgaatag cgcgtttcag cacagaggtg taagcaaagt caaagctgta accttcctct
     2281 ttcagcagct tacctgctgc ttttgcttcg cttacgcctt tctcagacag atccacgtcg
     2341 taccaaccgg tgaaacggtt ttctttgttc cactgacttt cgccatgacg aaccagaacc
     2401 agcttagtta cagccatata cttac
//
LOCUS       DAJOTB010000300         2423 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJOTB010000300 DAJOTB010000000
VERSION     DAJOTB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.303"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            65..490
                     /locus_tag="OHN28_004899"
     CDS             65..490
                     /locus_tag="OHN28_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2300137.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPITTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            487..837
                     /gene="tnpB"
                     /locus_tag="OHN28_004900"
     CDS             487..837
                     /gene="tnpB"
                     /locus_tag="OHN28_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2300138.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            868..>2423
                     /locus_tag="OHN28_004901"
     CDS             868..>2423
                     /locus_tag="OHN28_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCQ2300139.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLHRMLFGTRSEKLRREVELAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLEQVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKIHTDDTPVKVLAPGQKKAK
                     TGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLF
                     SAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEIRGLPESE
                     RLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSV
                     LPEWPSNRVD"
BASE COUNT          620 a          601 c          684 g          518 t
ORIGIN      
        1 tatgtagcca tcagacgaga attggtaact tagacgccca tctgatatag acggacatct
       61 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aactattcga atgaattcaa
      121 acttcgtatg gtggaactgg catcacaacc tggagcttgt gttgcacaga ttgcacgtga
      181 aaatggcgtc aatgataatg ttattttcaa atggctcagg ctctggcaga acgaagggcg
      241 tgtttcgcgg cgtcttccga taacgacctc ttctgacact ggcgttgaat tattacctgt
      301 agaaataacg ccggatgagc agaaagaacc tgtggcggcc attgcgccgt ctttatccac
      361 ttccactcag accagagtca gtgccagttc ctgcaaggtg gaattccgtc acggtaacat
      421 gacgctggaa aatccatcgc cagagctgct cacggtgttg atccgtgaac tgaccgggag
      481 gggaagatga tctcactccc atcaggtacc cgtatctggc tcgttgccgg cgttaccgat
      541 atgcgtaaat ccttcaacgg actgggagaa caggtacaac atgtgctgaa tgataatccc
      601 ttctccggtc acctgtttat cttccgtggc cgacggggtg acaccgttaa aattctttgg
      661 gctgatgctg atggtctgtg cctgttcacc aaacgcctgg aggaaggcca gtttatctgg
      721 cctgcggtac gtgacggcaa ggtatccatt acccgctcgc aactggcaat gctcctcgat
      781 aagctggact ggcgtcagcc aaaaacatcc cgccttaacg cactgacaat gttgtaaaaa
      841 acgcctggcc gcattataaa aacggccatg agtcagaaat acctcattcg cattgcagaa
      901 ctggaaagcc agctccgtca gaaagaccag caactgagtc tggttgaaga gacggaggcc
      961 ttcctgcgct ctgcactggc acgtgccgaa gaaaagatcg aagaagatga acgggaaata
     1021 gaacatctgc gggctcagat agaaaaactg caccggatgc tgttcggtac ccgttctgaa
     1081 aaactgcgtc gtgaagttga actggctgag gctctgctga aacaacgtga acaggacagc
     1141 gatcgttaca gtgggcggga agacgatcct caggttcccc gccagttgcg acagtcgcgc
     1201 catcgtcgtc cgttaccggc acaccttccc cgtgaaatac accgcctgga gccagaagaa
     1261 agctgttgcc cggagtgtgg cggtgagctg gattatctgg gggaagtcag cgctgaacag
     1321 ctggaacagg tgagcagtgc cctgaaagtg atccgcacag aacgggtaaa aaaagcctgt
     1381 acaaaatgtg actgtattgt tgaagcaccg gcgccgtccc gcccgataga gcgtggtatc
     1441 gcgggccccg gattacttgc ccgcgtgtta acgggaaaat actgcgaaca tctgccactg
     1501 tatcgtcaga gtgaaatctt tgcccgccag ggtgtcgaac tgagccgggc cttactctcc
     1561 aactgggttg acgcgtgctg ccagttaatg acaccggtga atgatgccct gtaccgttat
     1621 gtaatgaaca cccgcaagat tcacactgat gacacaccgg taaaggtact ggcaccgggt
     1681 cagaaaaagg cgaaaacagg gcgtatctgg acgtatgtcc gggatgatcg caatgtgggt
     1741 tcgtcatctc ctccagcggt ctggttcgcg tactcgccga accggcaggg gaaacacccg
     1801 gagcaacacc tccgcccctt ccggggtatc ctgcaggcgg atgcgttcac aggttacgac
     1861 aggttgttca gtgcagaacg tgaaggtggt gcactgacag aagttgcgtg ctgggcccat
     1921 gcccggcgaa aaatccacga tgtatacatc agcagcaaaa gtgcgacggc agaagaagca
     1981 ctgaagcgaa tcagtgaact gtacgccatc gaggatgaaa tacggggatt accggagtca
     2041 gagcgtcttg ccgtcaggca gcagcgaagc aaagtgttac tgacgtcgct gcatgaatgg
     2101 atggtggaga agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat
     2161 gtactgaatc agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggat
     2221 aataatgctg cggaaagagc gcttcgtgca gtctgtctcg gaaagaaaaa ctttatgttc
     2281 tttggcagcg atcacggcgg cgagcgtgga gcactgttgt acgggctgat cggcacctgc
     2341 cgtctgaacg gtatcgatcc ggaagcgtat ctgcgccata tcctgagcgt actgccggaa
     2401 tggccttcca accgagttga tga
//
LOCUS       DAJOTB010000301         2418 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJOTB010000301 DAJOTB010000000
VERSION     DAJOTB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.304"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..805)
                     /locus_tag="OHN28_004902"
     CDS             complement(<1..805)
                     /locus_tag="OHN28_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCQ2300140.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEE
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIR"
     gene            complement(1063..2316)
                     /gene="amiC"
                     /locus_tag="OHN28_004903"
     CDS             complement(1063..2316)
                     /gene="amiC"
                     /locus_tag="OHN28_004903"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCQ2300141.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT          653 a          584 c          491 g          690 t
ORIGIN      
        1 tacgaataga gagatatagt ttttcttcta aaattaattc atatttaaaa gtggcataca
       61 gataccgttc aatttcatga attgcgcgct gtaacaggat gtccttttca agaaactgtt
      121 catagatatc accaatatct ttctgtttta tttcttcatt gcataacacg cgacttgaaa
      181 aatatttaag gtgcatcata aatctggaat agttaaatga acttctgtcc acctttctgt
      241 tataaacaac ttcaataagc cccgtgatta tttgcgacaa ctcaataatt cgtactgatt
      301 cataatcaac tgaattttca taattattta aaatatgcaa tgctataaaa ccaatctcat
      361 catcaggaag ttgaacgctg aattgctcat taatgatagt taatgcatcg cgacttatcg
      421 catactctgc tttgtacaac gctttgattt catcaaggaa aacattatta atttgtatac
      481 ctgaagcgac tcgggatatt gcattcgaaa tgtgatccgc aagaatcaat agtagcgatg
      541 gattgagatt tttttcgaga tttttctccg catattgcac tattttttcg gcaacaaaca
      601 catactcaat atctacatgt tcaatcaatt tatagagttt gtttttttgt tcatttctga
      661 cataaaaaat ccggttagcc ggatgctcag ggaccgacat tccatatttt ttgttgtaac
      721 cgacgccagg cccagaaatg ataacctcct gtccattcat cgatgcctgt acacaattat
      781 tgttcatgac tttttcgatg atcattccat ggctccaaaa aaaaaggcaa ggcacgtcag
      841 aagtaataac tacttcaaac attgccttgc ctgatttaac agttacaaga tgattaacta
      901 atgcgtatac attacacgga attgtgctta attaaagaga tggtcggctt atttttcaag
      961 ccgacattca aatatccgac atcagtcaca aaaccagcgt ctaatattca ggtaaatgtt
     1021 atctattcga cgaaatataa attcattgaa taaataaaaa cattatcccc ttctcgccag
     1081 cgtcgcccca tcggcaaaat acgctttaat tcccgcaaga atagactccg caacttcctg
     1141 ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc
     1201 gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccagcct gttcaacttg
     1261 atttttatgc agcttgttga ttttaccgag cttattcagc accgctttac caaacttaag
     1321 gctgtcggca atggtcagcg actgcaccat atcgaacatg gtgtggtcga catagcggtc
     1381 accgcttttg cttacgccac caatcaagtc cgaggcgttc tgggtttgtg ccagatattt
     1441 tgccgcagta ctggttgcgc ctttggttga gagcgcaaac accgaggaac cgctcggctg
     1501 gcgactggta aaggcgtcgg catggataga aacaaacaag tcagcgcgct gtttctgggc
     1561 ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt
     1621 catattgccc tctttctcga tcagagagcg cagacggcga gctatttgca ataccacgtc
     1681 tttttcgcgt gttttgtatt tccccaccgc accggagtct tcgccaccgt ggccagggtc
     1741 aagcataatg acaatcggac gatcccgccc tgctttaccc ggttgtggac cactttgtgc
     1801 tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg
     1861 gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct ctttaaaccc
     1921 ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat
     1981 acgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtcagc
     2041 gcggatttgc gcagccatcc ccttgagcac tgagttcagg tttacatctt cgatatccac
     2101 caccacgcgt tcaggattac tcaacgcgaa ctgcttatat ttcagctgac gatttgattc
     2161 taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctgac
     2221 cgcagccagg ctgacctgac ttacgctcaa tagccacatg gcacccgcgc cttgcagtaa
     2281 acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct
     2341 aagctgtgta acaagtaaac gaccaatttg accggaaact ttagcgaaag acgcataatc
     2401 tgtcatctat aaaacggt
//
LOCUS       DAJOTB010000302         2411 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJOTB010000302 DAJOTB010000000
VERSION     DAJOTB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.305"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            22..714
                     /locus_tag="OHN28_004904"
     CDS             22..714
                     /locus_tag="OHN28_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2300142.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(763..2262)
                     /gene="pitB"
                     /locus_tag="OHN28_004905"
     CDS             complement(763..2262)
                     /gene="pitB"
                     /locus_tag="OHN28_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCQ2300143.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          658 a          610 c          574 g          569 t
ORIGIN      
        1 tttaaattta aggatatatt catgcagtca ataatacctc cattaattgc cgttattggt
       61 agcgatggtt caggcaagtc aacggtgtgt gaacatctta ttaccgttgt cgaaaaatat
      121 ggtgctgccg aaagggttca tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca
      181 aaattaccgt tgatgggaaa atccttacat aaaacaattg aacgaaatca ggtaaaaaca
      241 gcaaaaaaat tgcctggacc agttccggcg ctggtaatta cagcgtttgt cgcccgccgc
      301 ttactgcgct ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt tttaaccgac
      361 cgttatccgc aggaccagat tcctggcgct tatgatggta cggtgtttcc gcctaacgtt
      421 gaaggtggtc gttttgtctc atggctggca agtcaggaac gtaaagcatt tcactggatg
      481 gcgagccaca agcccgatct ggtcatcaaa ctcaatgttg accttgaagt tgcctgcgcg
      541 cgtaaacccg accataaacg ggaatcgctg gcgaggaaga ttgccataac gccacagtta
      601 acctttggtg gtgcacaact ggttgatatc gacgccaatc agccactgga aaaggtgctg
      661 gttgatgcag aaaaagcgat tacggatttt atgaccgcgc gtggttatca ctagtgaaaa
      721 atggaaatgc ccgatcgcca ggaccgggca ttttcaggaa ggttaaatca actgcaatgc
      781 tatccagtac aacccaccag aaagaaaaat cgccgccggt aaagtaaata cccacgccat
      841 caggatgctg gttaccgttt tacgctgtaa cccaccgccg tccaccacca tcgttcctgc
      901 tactgcagac gagagtacgt gtgttgtgga gacgggcatc ccaatataac tggcaaggcc
      961 gatagacact gccgccgtca tttgtgccgc catgccttgc gcatacgtca tgccgcgctt
     1021 accaatcttc tcaccgatgg tcatcgctac acgacgccag ccaatcatgg tgccgatgcc
     1081 gagcgccagt gctaccgcca tgatgatcca taccggtgcg tactcaatgg tgcttaacat
     1141 atcgctgcgc agttttttca gcaggttctg gtcttcttta ctgacgcctg gcagtttcgc
     1201 cagcttcgcg gaggtatcag agatgcacag cataatgcgg cgcagctggc tgcgctgact
     1261 cacgcttaac ggctcgtaac tttccatatt gcctggcagc atcgttttaa cgcgcgcaat
     1321 cgcatcaaag gtatttgccg gatgacagtg aaactctgtt acttgcgtgc catcagtcga
     1381 tgtcgcaggc aatggaggtt ccatcgtaat caacttctgc ggcagttcag gatgctgttg
     1441 caggtagtgt tcgaagttgg tgacggcatc gcgggtacgg gtaatttcat agccggacgc
     1501 attcatattg acgacaaacc cggcgggagc aatccccacc agcaccagca tcaccagacc
     1561 aattcctttc tgcccgtcgt tcgcgccgtg cgaaaacgcc acgcccgcag cggaaacaat
     1621 gagggcaata cgcgtccaga atggcggctt acgtttgcct tttttctttt tgcgatcttc
     1681 cggaatgcgg tgaatacggt cacgcttttt cgtcccactc cagaaacggc gtagcagaaa
     1741 aattagcccc cccgcaatca ccagccccac gatgggagag acaatgagtg aagagaaaat
     1801 cttggtgact tcgcgcaggt ttaacgcatc catcaccgac gaaccattta acagcgcgtt
     1861 ggttaaacca atgccgataa tcgcaccaat caaagtgtgc gaactggagg ccggtaaacc
     1921 aaagaaccac gttcccagat tccagataat cgccgccagc agcatggaaa agaccatcgc
     1981 cagaccgtga gttgacccca tattcagcaa caaatcggtt ggcaacatat ggacgatggc
     2041 ataggcaacg ctaagcccgc caagcaatac gccaaaaaag ttaaaaaatg ccgccatgac
     2101 cacagcaagc tgtggttgca tggcacgggt ataaataacg gttgccaccg cattcgccgt
     2161 gtcatgaaaa ccattgattg cttcatagaa caacacaaat gccagagcaa gtaataacaa
     2221 aagccccgta tatatatcaa ggccaacaaa taaatttagc atatattaga ttacgccatt
     2281 ttgaatttac gaacggaggc attatcagtg actttcacgg cgtgggcaaa gtgaaatatc
     2341 attttttaac gataaaaagc gtgttatttc ttgtgtatca gacaataagc cttcattcaa
     2401 aacctttaaa a
//
LOCUS       DAJOTB010000303         2398 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJOTB010000303 DAJOTB010000000
VERSION     DAJOTB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.306"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            845..1170
                     /locus_tag="OHN28_004906"
                     /pseudo
     CDS             845..1170
                     /locus_tag="OHN28_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1318..2398)
                     /locus_tag="OHN28_004907"
                     /pseudo
     CDS             complement(1318..2398)
                     /locus_tag="OHN28_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(2134..2250)
                     /locus_tag="OHN28_004907"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          580 a          606 c          558 g          654 t
ORIGIN      
        1 ccctctcgct ttgtttcaac agtattacta attaccggga aagcctgaca agatttcgta
       61 ttaaggtgtt atctacgccc attatacttt aatacatcaa gtaattaatt cactaacctc
      121 tctttcgttt cactggactt tacggctgta aaatagcttt tatcttgcag atgaaaactc
      181 tgctcaggaa agtgccttta gttctaatgg atggtgagtt ttacgcttgt ctttgtaacc
      241 aggaagagag gcgtagacag taaagttcct cgctccctca tcttggcatt cggtgaacta
      301 tagacaagag tcaataaacc aaactgcaac aacatagtca ttttgggagt aagattatta
      361 gtcttaacgt ctcatatagc tggtatgaag aaattttcat gtatacttga taaacgtatc
      421 gtcaacaacc gataaaaagt gagccattta tatctccatc aatgatctaa tattgaatcg
      481 ccacgggtag tcttactcct tccaagttgt tgataataat tgtttttata ttctattgtt
      541 gacatctgtt cgcaggaacc atgccgacgc ttattgttat aaaacatttc gatgttcaaa
      601 aatatcgttg gaggcgtttt cccgcgttcc gaagagcttt ttctttatcc gttcacgctt
      661 cagtaactgg aaaaaattct gcaagcgtat cgtggtaatt actgcgacgg agcatacact
      721 cctccaggtc ttatgatttc aggaacgact gtcattcatg gcttgtttac tgacaaccag
      781 catcgttttt ggggattacg ccgctataca ggtgtggtgc ggtgatgtga cctatatctg
      841 gacgggtaag cccggcgggg tgatgttcca cagcgatacg agcaggcagt tccggcagtt
      901 attgtggcga taccagatca ggcagagtat gagccggcgc ggaaactgct gggataacag
      961 cccaatggaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg tgggttacga
     1021 agcttctgcg aggccgctca cgccataacg gtctatatcg ttggatatta cagcgcacta
     1081 agaccgcacg aatataacgg tgggttatcc ccaaacgaat cggaaaatcg atactggaaa
     1141 aactctaact cggtggccag tttttgttga ccacttcaca cgttcaatgt cgttgatgac
     1201 tttaactatg aggcgttgtc gattgaaaac aatctgaatc tgccagcttc acaagtggta
     1261 gcggtactct gtaggtgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca
     1321 ggctgccaga tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg
     1381 agtatggccc agcctttcca gcaatcgtcg attgttatac cagtccaccc acgtgagtgt
     1441 ggccagttcc acttctgcac ggtttttcca gctcttacgg tgtattacct ccgctttgta
     1501 aaggccattg atgctctccg ccatcgcgtt gtcatacgag tcgcctgtac ttcctgttga
     1561 tgccagtaat ccggcttcct taagccgctg tgtgtaggcc agcgatacat actgagaacc
     1621 tttatcactg tgatggactg tgccggacgg tcgacgggcc cataacgcct gctccagagc
     1681 atccagcaca aatgtcgttt ccatagacga tgagacccgc caccccacga tgtatccggc
     1741 aaacacatca atgatgaacg ccacatagac gaccccctgc catgtgctga cgtaagtaga
     1801 atcagccacc cacaactggt caggacgttc tgccacgaac tgacggttta cgcggtcgcc
     1861 tgcgacaacg gctttccggc tgatggtcgt acggaccttt ttaccacgga gaacaccggc
     1921 aagtcccata accgccatga gacgtgccac agtgcatctg gccactctga taccttcccg
     1981 taacaactga cgccagactt tacgcacacc gtataccttg tgattttcat cgtatacgcg
     2041 ctgtatctct ttcttcagcc agtcatcgcg ctgcgcacgg gcactgcgtt tatccggatg
     2101 atgtcgctgt tgctgacagt ggtaatacgt tgacggggca atatgcagtt cgctgcatag
     2161 cggtccgacc ccgtactgct cacgcagctt atccagcagt ggcatcattt tttccagagg
     2221 cggtcgaact ccgccttcgc aaaataagcg gaagcctggc gaaggatatc gttactgcgg
     2281 cgcagttcac gattttcacg ttccagctct ttcagacgct gacgttcagc ggtggtgagc
     2341 ccgccatcac cgcctccggt atcccgctca tgctggcgta cccagacacg cagagtct
//
LOCUS       DAJOTB010000304         2364 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJOTB010000304 DAJOTB010000000
VERSION     DAJOTB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.307"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..404)
                     /gene="dfrA"
                     /locus_tag="OHN28_004908"
     CDS             complement(<1..404)
                     /gene="dfrA"
                     /locus_tag="OHN28_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCQ2300144.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPEN"
     gene            562..1575
                     /gene="intI1"
                     /locus_tag="OHN28_004909"
     CDS             562..1575
                     /gene="intI1"
                     /locus_tag="OHN28_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCQ2300145.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1778..2128
                     /locus_tag="OHN28_004910"
     CDS             1778..2128
                     /locus_tag="OHN28_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCQ2300146.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2254..>2364
                     /locus_tag="OHN28_004911"
     CDS             2254..>2364
                     /locus_tag="OHN28_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2300147.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASG"
BASE COUNT          504 a          653 c          656 g          551 t
ORIGIN      
        1 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
       61 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
      121 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
      181 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
      241 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
      301 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
      361 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
      421 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
      481 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
      541 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
      601 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
      661 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
      721 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
      781 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
      841 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
      901 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
      961 cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg
     1021 cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc
     1081 aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagctgtcg
     1141 cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc
     1201 gacgcccttg agcggaagta tccgcgcgcc gggcattcct ggccgtggtt ctgggttttt
     1261 gcgcagcaca cgcattcgac cgatccacgg agcggtgtcg tgcgtcgcca tcacatgtat
     1321 gaccagacct ttcagcgcgc cttcaaacgt gccgtagaac aagcaggcat cacgaagccc
     1381 gccacaccgc acaccctccg ccactcgttc gcgacggcct tgctccgcag cggttacgac
     1441 attcgaaccg tgcaggatct gctcggccat tccgacgtct ctacgacgat gatttacacg
     1501 catgtgctga aagttggcgg tgccggagtg cgctcaccgc ttgatgcgct gccgcccctc
     1561 actagtgaga ggtagggcag cgcaagtcaa tcctggcgga ttcactaccc ctgcgcgaag
     1621 gccatcggtg ccgcatcgaa cggccggttg cggaaagtcc tccctgcgtc cgctgatggc
     1681 cggcagcagc ccgtcgttgc ctgatggatc caacccctcc gctgctatag tgcagtcggc
     1741 ttctgacgtt cagtgcagcc gtcttctgaa aacgacaatg gaggtggtag ccgagggtgt
     1801 ggaaacaccc gactgccttg cgtggttgcg gcaggcgggt tgcgacacgg tgcagggttt
     1861 cctgttcgcc aggccgatgc cggcggcggc cttcgtcggc ttcgtcaacc aatggaggaa
     1921 caccaccatg aacgccaatg aaccgagcac cagttgctgc gtgtgctgca aggaaatccc
     1981 gctcgatgcc gccttcacgc cggaaggggc cgagtacgtg gagcatttct gcgggctgga
     2041 gtgctatcag cgcttccagg cgcgggccag cactgcgacc gaaaccagcg tcaaaccgga
     2101 cgcttgtgat tcgccgccgt caggttgagg cataccctaa cctgatgtca gatgccatgt
     2161 gtaaattgcg tcaggatagg attgaatttt gaatttattg acatatctcg ttgaaggtca
     2221 tagagtcttc cctgacattt tgcagggaat tccatgactg gacagcgcat tgggtatatc
     2281 agggtcagca ccttcgacca gaacccggaa cggcaactgg aaggcgtcaa ggttgatcgc
     2341 gcttttagcg acaaggcatc cggc
//
LOCUS       DAJOTB010000305         2310 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJOTB010000305 DAJOTB010000000
VERSION     DAJOTB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.308"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(106..1005)
                     /gene="lysO"
                     /locus_tag="OHN28_004912"
     CDS             complement(106..1005)
                     /gene="lysO"
                     /locus_tag="OHN28_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCQ2300148.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(1420..2115)
                     /gene="aqpZ"
                     /locus_tag="OHN28_004913"
     CDS             complement(1420..2115)
                     /gene="aqpZ"
                     /locus_tag="OHN28_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCQ2300149.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT          735 a          568 c          555 g          452 t
ORIGIN      
        1 aaggttaaga cgcttaacgc ggggataaaa gagattttta atgcaacttt aagggagatt
       61 gatttagcgc aattttggcg gcagagatct accgccagag aggtattatg cagagaaaaa
      121 ggcgatgaga atcggcacta acagactaag aataaaaccg tgaacaatcg ccgccgggac
      181 catatccagc ccgcctgtac gttgaagaac gggcagggtg aaatccatcg acgtcgcacc
      241 gcataagccc agtgcagtag agcggctacg gcgaatcagc ccagggatta acataatagc
      301 aatcagctca cgggccagat cattaaaaaa cgccgcgctc ccgattaccg gaccaaaaga
      361 ttcggtcaat aaaataccgg aaagagaata ccagccgaaa ccggaggcca ttgccagcgc
      421 ggtattgatg ggaagatcaa gaataaaggc gttaattaaa ccaccaatta atgaactggc
      481 aaccaccacc acggcgacaa tcattccccg gcgattaagg acaatctgct ttaaggtcat
      541 gccattattg cgcaactgaa taccgacgag gaaaagcagc agaattaagg tgtattcact
      601 ggcttcggtc gcgtgttgta agaaagccag tccactcaga ccaatggcaa aaccaatcac
      661 tactacgccg cacagtttta gcgactccag cgccatcgca atacgcgacg ggagtttttc
      721 ttgctgatgg tggttgctcc atggcaggcc gcgctccagc cacatcaggg cggcaatatt
      781 acacagtaaa ataacggtag tactgacggc agaataatgc agaattgcca acaagttact
      841 ggcgaggtta tcgagaaacg ccagactgat acccataaaa aagagaataa ggtaaaccat
      901 ccagcttaag agctgattaa taactcttaa tgcagctttt tggcgaagcg gaatgaggta
      961 acccacaatc aggggaacca gaatgattaa cagcccagaa aacatgaaaa cccagtcctt
     1021 gcaaagaaga ttcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt
     1081 gcaattaaaa ggaaatgtta tgcataagga tcggtagggt attcattttc atttaaagat
     1141 attcttgcgc tttaattaca aaccgcaccg gtgttggtgg cgtcaaaatc gccgacccgg
     1201 tagccgtaag ggataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg
     1261 gggtgcaggg ggcggcggcg attggccgcc ccctgcacgc tccttgcgcc agtggcaata
     1321 tgtggcttag ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt
     1381 aaaaaaaggc ctgacattgc gccaggcctt ctgcgttaat taattacgct tttccagtag
     1441 agtccggtaa atcagaccac cgataatacc gccgacaatt ggcaccaccc agaagaacca
     1501 cagttgttct aatgcccagc cgccctggaa gatagcaacc gcagtactgc gcgccgggtt
     1561 aacggaagtg ttagtcaccg gaatactgat taagtgaatc agggttaagg ccagaccaat
     1621 ggcaattggc gcaaaaccag caggagcgaa tttgtcagtt gcgccgtgga tcaccagcag
     1681 gaaacctgca ctcaatacca gttcaactac cagcgcggaa agcatggaat aaccgcctgg
     1741 tgaatgctcg ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt
     1801 accactggca attaaataca gcagcgccgc cgcaacaata ccgccgacaa cctgggcaat
     1861 tacgtagcca acgacttctt ttgccgggaa acgtccgcca gcccataaac caatagtgac
     1921 cgccgggtta aaatgaccac cagaaatatg accaacagca aaggccattg tcagaacggt
     1981 cagaccgaac gccaacgcca cgccggcaaa accaatgcct aattccggga agcctgcggc
     2041 cagtacggca ctaccacagc cgccaaaaac aagccagaaa gtaccaaaac attcagctgc
     2101 taattttctg aacatatcca cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt
     2161 tatagggtcg tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt aaaaacgaag
     2221 gcaaatcatt cgccagataa ataaatccag taaatttgat ttagggcaac agcgggttgc
     2281 cccatatagt catttgtctg attgacagtg
//
LOCUS       DAJOTB010000306         2189 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJOTB010000306 DAJOTB010000000
VERSION     DAJOTB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.309"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(363..1283)
                     /gene="lpxP"
                     /locus_tag="OHN28_004914"
     CDS             complement(363..1283)
                     /gene="lpxP"
                     /locus_tag="OHN28_004914"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCQ2300150.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            1636..1878
                     /locus_tag="OHN28_004915"
     CDS             1636..1878
                     /locus_tag="OHN28_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCQ2300151.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(1955..>2189)
                     /locus_tag="OHN28_004916"
     CDS             complement(1955..>2189)
                     /locus_tag="OHN28_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300152.1"
                     /translation="SIMACNVFAFSISGGGSEASYKETEKTSVMTTTHSTKLQPSQAI
                     LFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
BASE COUNT          565 a          492 c          523 g          609 t
ORIGIN      
        1 tttcacttta catctctccg cgtaattact gttacgcctg aatgacttca ttacacctga
       61 tacagtgagt tgacaccggg caaaacgacc ttaaacgtcc tgtattttac cgtgggcatc
      121 atcccagaat tctgagctaa actgctcgag acggcgctaa tgtacgccta aaactgaaac
      181 gagacaagag gcattttctt atttactttt agtaacttac aggtgtctca tatcggcgac
      241 agtttaattt ctgacggtaa taattagata ttcagcgaag gaaaaaacct taagaaaaaa
      301 agccataaaa accatgaggt tattatggct gatttgagga gggaaagagt gagagccgtt
      361 tgttaaatgt ataacgacga ttcacccacc gggcgcgttt taaagcgacg gtgtatccag
      421 aggtactgct ccggtgcgcg catgatctct ttctcgataa tcttgttcat ataggcagcg
      481 gcttgatttt catctgtcgg gtagccttcc atctctgggg tgatgtacaa acgatatccg
      541 ctgtaatccg cttttcttac catcgttacg gtcaacatgg ctgcgccaga gagacgagag
      601 aggacatagg tgccattggt tgtggcgaca ttttccaccg caaagaacgg cgcgaaggag
      661 ctgcctttac gaccataatc ctgatcggga gcaaaccata ccgcttcacc tttcttcagt
      721 gcactgacaa tgccgcgcag attatttctg ccgatcatcg ctttgttaga gcgcatgcga
      781 ccacgggtct ggacccattc catcagctga ttattatgcg ggcgataggt ggccatcatt
      841 ggttggcaaa gtcccatcac ccgaccgccc agttccagcg acataaaatg gacgccgaca
      901 accattacgc cgcgattttg catttgtgcg cgtttaaggt tatccagccc ttccacatca
      961 aaccatttac gtacgcgact gtcgggccag aaccacgcca tgccggtttc taccagcgcc
     1021 atgccgagtg aacgaaagtt ttcggcaatc atcttctcgc gctcttccgc agaatgctgc
     1081 gggaaacaaa gttccaggtt tttacgggcg atagattcac gacgtttcag gaatggtcgc
     1141 gccattgtgc cgatacgcgt gccgagaaag cagagaacag gataaggaag ctgtacccaa
     1201 agccagagta caccaagccc aaaccacgtg agccagtagc gaggatgcag aaactcgcgg
     1261 gaaaatttgc attttggaaa catagttacc ttctgtagat acgaattgtt gtatcgcaga
     1321 agattccatg tcgacagcgg tacactcaat tgattaggcc atcatgtacg acaatggttc
     1381 ctgtaaagag tgtaaagact agtcaggaca taaccacgag gagcatgaat gcggtatctg
     1441 tactttatca ctcgctgact gctttcagct actgattatt aatcgtgcaa agtaaatatt
     1501 tttccgtacg taacagtctt gcatttaaca tgctgttttg attgatgctt agcaaaaatt
     1561 gccagaggtt aaggattgtc ttactattaa agtgactgaa gttacaggtt ttttagcgcg
     1621 gcaggaggtt gagctatgat ttatttatgg atgtttctcg ccctgtgtat tgtctgcgta
     1681 agcggataca tcggtcaggt actgaacatg gtatcggcga tctcttcttt cttcggcatg
     1741 gtgatcctcg ctgcactcat ttattacttc accatgtggt taactggcgg taatgaactg
     1801 gtgacgggga tatttatgtt tcttgccccg gcttgtggct tgatgattcg ctttatggtg
     1861 gggtatggca ggcgatagtg ggtcaggatg gctcatttgg gcaggtgttc acatcagcat
     1921 cagacatgat tgttcacctg ccaggtgaca gacgctatcg caaaggatga atgagataat
     1981 tcgcctttgt cggataagta ggcgtgatgt cttctgtgag gtttaatggt ggtgcatctt
     2041 ctctcatctt aaacaaaatc gcctgtgatg gctgaagttt tgtagaatgc gttgtcgtca
     2101 tcactgacgt tttttctgtc tctttatagc tcgcctcact accaccacca gaaatagaaa
     2161 atgcaaaaac attgcatgcc ataatagag
//
LOCUS       DAJOTB010000307         2163 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJOTB010000307 DAJOTB010000000
VERSION     DAJOTB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.310"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(66..1112)
                     /gene="aroH"
                     /locus_tag="OHN28_004917"
     CDS             complement(66..1112)
                     /gene="aroH"
                     /locus_tag="OHN28_004917"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCQ2300153.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1269..2102)
                     /gene="ppsR"
                     /locus_tag="OHN28_004918"
     CDS             complement(1269..2102)
                     /gene="ppsR"
                     /locus_tag="OHN28_004918"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCQ2300154.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          535 a          540 c          563 g          525 t
ORIGIN      
        1 agtaatttgt tgagaagaca acaagttgcg cagaaatgcc catcctgtca ggaatgggca
       61 cgcattcaga agcgggtatc taccgcagag gcgagttttt cgaccaggcg ttcggtatct
      121 tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg accgccgacg
      181 attttttgcg ttccttcgcg caggaaactt tccgccataa ttccggcaat cgccgtagag
      241 ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc
      301 tggcagttac cgtggctgaa atccaccacc agatgttcag gcaaatcaaa ttcgtgcagc
      361 gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata
      421 ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc gtttttgtct
      481 ggcgagagga acatatggct agcgcgggct gcgcggatag catccacggc aatccgcgtg
      541 ttgccatcgg taccattttt aaaacccacc ggacaggaga gtgccgaagc catttcgcgg
      601 tggatctgac tttcggtagt acgtgcgcca atcgcgcccc aactgattaa atcagcaata
      661 aactgaccgg tcaccatatc gaggaactca gtcgcggtag ggacgcccag ctcatttacc
      721 tgtaaaagta atttgcgcgc cagctccaga ccgtgattta cccgatagct accgtttaaa
      781 tctggatcgg agatgagtcc tttccagccg acaaccgttc gtggtttttc aaaataggtg
      841 cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg
      901 tactccatcg cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc
      961 ttatcttcac cattcagtat tttttcaatt ctacggcggg agtcggtgac atgggtggcg
     1021 acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata
     1081 cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta
     1141 cggcaggact accgggaagt gatgcgcatc acgataaacc aatcgcagct gatttcaagt
     1201 gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaaca agctaatgca
     1261 ctagttctct agtacattcg gcggctaagg cccatgatat cgaggatctt ggtggcaatc
     1321 tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg gtacaacgct
     1381 tccacttccg cgacttccat cctgcactga cgaagcgagg catagcgact gttctcccga
     1441 cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc
     1501 tgaagtggtt tgagcgacgc gggtagcacc agattatcca tatcatcggc aataaagggg
     1561 tagtttgccg cgcggatacc aaattgcatt gccagataca gactggtggg ggttttacca
     1621 cagcgagaaa caccgagcag gatcacctga gcctggtcca gattgcgcaa cgaaataccg
     1681 tcatcgtggg caagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta
     1741 ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt
     1801 tgtagtgggg caaccagcgc ctgaacgata tcctggcaaa agccttcact ttgcaagatg
     1861 atagcgcgaa tctccggcaa cacgatggag tagaagacca gcgggcgcac acctgtctgg
     1921 tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac
     1981 ggcagcgtaa tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc
     2041 tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc
     2101 attttgttct tcccgtgatg cagacataat tctatgaaag cataaattaa aaacgcacag
     2161 aag
//
LOCUS       DAJOTB010000308         2045 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJOTB010000308 DAJOTB010000000
VERSION     DAJOTB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.312"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            70..318
                     /locus_tag="OHN28_004919"
     CDS             70..318
                     /locus_tag="OHN28_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300155.1"
                     /translation="MNNSIPERFIFQCALFKNLEKEVFMTHGYVDSHIIAHALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            616..1509
                     /locus_tag="OHN28_004920"
                     /pseudo
     CDS             616..1509
                     /locus_tag="OHN28_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1641..1859
                     /locus_tag="OHN28_004921"
     CDS             1641..1859
                     /locus_tag="OHN28_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300156.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          545 a          389 c          442 g          669 t
ORIGIN      
        1 tatttttgtc tgtcgcttta gactatacat agcataattt tattgggtca ctattcctgc
       61 gaggctgaca tgaataattc aatcccagag agatttattt ttcaatgtgc tttgtttaaa
      121 aacctcgaga aggaggtgtt catgacacat ggatatgttg atagtcatat tattgctcat
      181 gctttacggt tgcgtttaaa agatgagacc agtgttattc tttctgatct ctatctgcaa
      241 atcttgcagt acattgaaat gcataagact acactaacgg atatcattat taatgacagg
      301 gaatccgtgc tctcttaaaa cttgagtaag gtttttgaat aaacatcagc tgtatcacta
      361 tgctgatgca aagtgagcat ttcaggcgtt atgctttctt aatatatccg caatatcaga
      421 gggcaaacag gaatggagag cgctcgtttt ccattcttga cgcctgatat cccgcctaat
      481 ttgtttaatt ctgcctacat tgttctaaat aattaatagg ttagatgaag ttggagaaaa
      541 ttttatcttc ggcgtggata tttgtgatgc tctctaccat gctatctgta gaccccagcg
      601 cgagcgaatg aacgtctctt tagcttctac agacttatcg ataaaggctt tgggattttg
      661 atatttattc tgttaatcgc ctgcgccgct gccttccttc ttgataggta tttcaataaa
      721 agcgcaacgc ctgaagagat cctgcgacgg gctataaata atggggagat cgtccctttt
      781 taccaacctg tggtaaatgg tcgggaaggg acattgcggg gagttgaggt gttagcccgc
      841 tggaaacaac ctcacggtgg atatatatca cctgcggcat ttattccact tgctgaaaaa
      901 tgcggattaa tcgttccgct tacgcaaagc ctgatgaatc aggttgccac ccagatgaac
      961 gctatcgcga gtaaactgcc ggaaggtttt catattggga ttaattttag cgcctcgcat
     1021 attgtttcgc cgacgtttgt cgacgagtgc ttaaattacc gtgacagttt tacccgccgc
     1081 gatttaaacc ttgttctgga agtcaccgag cgtgaaccat taaatgttga tgaaagtctg
     1141 gttcagcggt tgaacattct gcatgaaaat ggttttgtca tcgcgctgga tgatttcggt
     1201 actggttact cagggctttc ttatctgcat gacctgcata ttgattatat aaaaattgat
     1261 cacagtttcg ttggccgcgt caacgcagac ccagaatcaa cccgaattct ggattgtgta
     1321 ttggatctgg cgcgtaaact ttcgatcagt atcgtcgctg aaggtgtcga aacgaaagaa
     1381 caacttgact atctgaacca aaataatatt acatttcagc agggttatta tttctataaa
     1441 cctgttacat acatcgacct ggtcaaaatt atcctttcta aaccgaaggt gaaggttgtg
     1501 gttgagtgaa aattgatcag taaggccatg gtacggtgta attatagaca gctaattagc
     1561 tcgttgcctc ttgttactat tgttcattct tttgtttgct ataattgttt gaaagttttg
     1621 actggattgc cattagtagc atgaacaata gtaataatct ggattatttc actctctata
     1681 tcatcttttc cattgcattt atgttgatca ctctcctggt catccttatt gcaaaaccca
     1741 gcaccgggct gggagaagtg cttgtgacga taaatttgct taatgccctt gtttggctgg
     1801 cgatcaatct ggttaatcga ttaagggaaa gactcgtcag ccacagggat cagcactaat
     1861 ctttcagttt ctcactgtca gtatgcggct gaatgggttg ctggcagtga acccctggat
     1921 cattgaagga aaggaattat tgtgcaaata gttgtcaacc ctggtgttat cacagttgtt
     1981 ttatatatca gtgaaataat ttctatggca actgttgata attttgatgt cgaagagtta
     2041 tttgt
//
LOCUS       DAJOTB010000309         2022 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJOTB010000309 DAJOTB010000000
VERSION     DAJOTB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.313"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..227)
                     /locus_tag="OHN28_004922"
     CDS             complement(<1..227)
                     /locus_tag="OHN28_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCQ2300157.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSSMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENIL"
     gene            complement(230..2011)
                     /gene="hcp"
                     /locus_tag="OHN28_004923"
     CDS             complement(230..2011)
                     /gene="hcp"
                     /locus_tag="OHN28_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HCQ2300158.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFVQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEF
                     REALWLEVSQEPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIAI
                     EYGGGVYDIDNLRIVTPRLHDEIHYRR"
BASE COUNT          529 a          500 c          448 g          545 t
ORIGIN      
        1 ttcaggatgt tttcaggctt atcatctcct tgacttttag ggtagaaaat tagatcgctc
       61 cctgacggat gctgtgtaat ttcaataaaa tgatctacta atgaatccca gtacatttct
      121 agctccttac cttttaatgg ctttccatct ttcatagatg atctcaatcc acctaaaaaa
      181 ttaagaaatt cctcctcggt ataatcttcg agcttttctt taaacatatt tatctcctgt
      241 agtgaatctc atcgtgtagt ctgggggtaa caattcgtaa attatcaata tcgtatacgc
      301 cgccgccata ttcaatagca ataaaatgat gtatctgaaa ttttttaagt gtttctttag
      361 gaccaatata gtgtccttcg tcgggggcaa aaggtgtaaa gccctgacta atacgtaact
      421 gattaatttc cgaaaactgc gctatcaact ccggttcctg actcacctcc agccacagcg
      481 cctcgcgaaa ctcatcaaag gttttaaact cccgtccgcg caatttatca acaatatgcg
      541 ccgggattgg tgcgccgttg ccgttattgg ataccgacat atccagccag cgagttcctg
      601 gtgaaagcgg gacgccgtgc cctgttacaa caccaggcag ctttcgcgga tcctccttca
      661 ggtaaacata gataggctta ataccggatc ccgccggaaa aaccagaata taatcgttga
      721 attctttcgg atcaggaacc gggaatggcg gcgtggtata ggtatcggtg ccgtccggaa
      781 tcggcgtcac cagaatggtg ggctgctcca gcggcggcct gtcgttaccg gtatggcccg
      841 gaacattccc atgttccgga ttttccggcg tccaggtaat cagggggcca tcgacatcgt
      901 gagcgggggt aaattcataa cgctgcgtct gctcgttcca ctgcatgttc gccacgcgga
      961 cgttttcata cggcgtacct tcacccgtat gaacaccata cacctgaggc ttgccgtgaa
     1021 tgtcagttcc ccagaaaaaa cgcacccggg tggtggcggt actgcctgct agtgctttct
     1081 gccgcagttc ttcctcatac cagaactcat cacgtccagg cacacgagca ctgccctcac
     1141 ccgcagaggg aatatacaga gcagatgcca acgcaccgat gtaagagccg cgggttgcac
     1201 tgagtacgcc actgagcgac cattttcccc atatttgagt cgccaacaca ctactcggga
     1261 acagtagcat ggtttgagct tctgcgggtt ccccgataac gttagcctcg ggatggtggt
     1321 atccacagca gtcatcaacc actggctgaa cgaaaatcgc tacttgaccg aagttttcag
     1381 cgggttcttc tgccgttccg gcatctgtac atcccttcac cttcagacag gatttggcat
     1441 agaccgggtt aaccgctggt gaagagagtg gaaggttaac aactggtagc ggttcaggct
     1501 taagattact ggcaggtgga cttttaggcg gaataaataa gctattatag ccagcaggaa
     1561 aggccaacca gtcaaattcc gtattggcta tgagatgttt acataatatg tagtcataat
     1621 cgacagttac acattcggta ggaagattat tgtcagaaat gtttacatga atagcagtta
     1681 atgaagcatt tcttagctga atataataat accgttccca tcgcccgctt ttatttatac
     1741 gatacaaatc gatttcaata aataaccgct cattctgatt gatggcatta gacagtagcg
     1801 gtgagctttt atcaatgagt ttgcaaaatt gtagcccctg tagattaacg ccattaccag
     1861 tactggtaat ggcgttggtg agtgcaaagg caaaaatttc atcttcatgg ccaacctgcc
     1921 agcgattacc aaccgacgca tacgtcccac agcctgaaga aatatcgccc tgcttctcac
     1981 cgattattct caggtaaaca atatcgccca ctttatttca tt
//
LOCUS       DAJOTB010000310         1963 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJOTB010000310 DAJOTB010000000
VERSION     DAJOTB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.315"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            11..1192
                     /locus_tag="OHN28_004924"
     CDS             11..1192
                     /locus_tag="OHN28_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2300159.1"
                     /translation="MEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTNLSLS
                     VWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLMATGA
                     VTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSCGRLF
                     GPLCAGAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDCLPYL
                     LCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVVLRPQ
                     RLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLAD
                     GAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTI
                     K"
     gene            complement(1572..1952)
                     /gene="crcB"
                     /locus_tag="OHN28_004925"
     CDS             complement(1572..1952)
                     /gene="crcB"
                     /locus_tag="OHN28_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCQ2300160.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
BASE COUNT          460 a          476 c          536 g          491 t
ORIGIN      
        1 gtcatctaag atcgaagata cgccccaaaa aaccctgtcc tgctggccac tggcgttcag
       61 tgccggtctt ctcggtatcg gacagaacgg tctgctggtt gtactccctg ttctggtcat
      121 acagacaaat ctgagtctgt ctgtatgggc tgccctgctg atgctgggct caatgctgtt
      181 tctgccatct tccccatggt ggggaaagca aatttccctt actggcagta agactgtggt
      241 gctgtgggct ctgggaggat atggcgtaag ctttaccctg cttgggctgg gaagcgtgct
      301 gatggctacc ggtgccgtaa caacagcggt ggggttggga atattaatca tcgcccggat
      361 cgtctacggt ctgaccgtgt cagcaatggt gccagcctgt caggtctggg cattgcagag
      421 agcgggagaa gggaatcgca tggccgctct ggcaaccatc agctccggcc tgagctgcgg
      481 caggctattc gggccgctgt gcgcgggggc aatgttggtc attcaccctc tggcgccagt
      541 gtggatgctg atggcagctc cggtgtgggc tgtggtgatg cttctgcggt tgcccggcac
      601 accaccacag cccacaccgg agcgcaagag cgtcagcctg aagcgggatt gcctgcctta
      661 tctgctttgc gcaatgttac tggctgcggc aatgagcatg atgcagcttg gactttcgcc
      721 agcccttact cgccagttcg atactgatac caccactatt agccaacagg tggcgtggtt
      781 gttggggctg tccgcaatag ctgcgcttat cgcgcagttc gtggtactcc gtccacagcg
      841 cctgactcca gtggctctgc tcctgagtgc cggggtgttg atgagtagtg gtctggctat
      901 catgctcact gaacagctat ggttgtttta cctgggctgt gcagtgctgt catttggggc
      961 cgctctggca acacccgctt atcaactttt actgaatgat aagctggccg acggcgcagg
     1021 cgcgggctgg cttgcggcca gtcacacact tggctatggg ctgtgcgcat tgttggtgcc
     1081 actggcgtcg aaaacaggtg tctcaatcgc gctgattgtg acggcattat ttgccgctgt
     1141 attatttacc atagtgtctg catgtatttg gcattaccgt acaatcaagt aaggctatga
     1201 tgttgtactc acagctggca taacgtcctg tgcaatttcg ctgtcgaaac cgcctgtgct
     1261 gaccgcccag tgcactcacc tgaagcacat cgcagcatgc caccaggatg ccagatcgtc
     1321 ttctgaaatg ggtggataca ttggcaacgt taaccgcaac aatttttcag agagaatttt
     1381 ggggggtgag ggccagtgat gtaaaaaata cgccaagagg ttaattcatt gtttatactg
     1441 atatgtcagg taatgcgttt gcagtcatta cagtcgtcat ggagactgta atgactgcaa
     1501 acgcatacca tggagcgaaa ttctatccta agcgtcacat tagttgataa acctgtaacg
     1561 ttaatgaatt ctcatgcgaa aagtatggtt atgataaaaa agcctaaagc tgtcatgatg
     1621 agggagccaa tgacgtgaac aagtacagac gtcaaagccc atatataatt tcccgcctgg
     1681 agtaatgcga atacttcaac agaaaatgta gagaaagtac tcagacctcc acaaagtccc
     1741 gttgttatca ttaatttcca aaaaggatca aggtgtggtt gtcgtaaaaa gtacgccaac
     1801 gcagtaccaa taattaaccc ggcaaggagg ttcaccacca gtgtacctgg aggtagattt
     1861 ggaaaaagac tgttaaagcg tgtggacagt aaccagcgca gagtacatcc aacagaaccg
     1921 ccgataatga cggcaaaaag tgatttaatc atgtacccct cat
//
LOCUS       DAJOTB010000311         1958 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJOTB010000311 DAJOTB010000000
VERSION     DAJOTB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.316"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            16..1698
                     /gene="glcA"
                     /locus_tag="OHN28_004926"
     CDS             16..1698
                     /gene="glcA"
                     /locus_tag="OHN28_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCQ2300161.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT          399 a          472 c          513 g          574 t
ORIGIN      
        1 aagagactga acaatatggt tacctggacc caaatgtata tgccgatggg aggactgggg
       61 ctatccgctc tggtcgccct gatcccgata atattcttct tcgttgcact cgcggtatta
      121 cgtctgaaag gacatgtcgc tggagcaata acccttatat tatctattct gattgcgata
      181 ttcgccttta aaatgccgat tgatatggca tttgctgctg cgggctatgg ctttatttat
      241 ggtttatggc ctattgcgtg gattattgtc gcggcggtat tcctgtataa attaaccgtt
      301 gccagcggcc agttcgatat tatccgcagc tcggttatct ccatcaccga cgatcagcgt
      361 ttgcaggtgt tactgattgg tttctccttt ggtgcgctgc tggaaggggc ggctggcttt
      421 ggcgcaccgg tggcgattac cggtgctcta ctggtgggcc tgggcttcaa accgctatac
      481 gcggcggggc tgtgtctgat tgccaacact gcgccggtgg cgtttggtgc gttgggtgtg
      541 ccaattctgg tcgccggtca ggtaacggga atcgatccgt tcgacattgg cgcaatggcg
      601 ggacgtcagt taccgttcct gtcggttctt gtgccgttct ggctggtagc aatgatggac
      661 ggctggaaag gggtgaaaga gacgtggcca gcggcgctgg ttgcaggggg aagcttcgct
      721 gtcactcagt tctttacctc taactatatt ggtccggaac tgccggatat tacttcggcg
      781 ctggtgagta tcgtctcact ttctttattt cttaaagtgt ggcggccaaa aaataccgaa
      841 acggcaatca gtatgggaca atccgcaggt gcgatggtgg tgaataaacc gtcttctggc
      901 ggtcccgtgc cttcagaata tcgtctgggg caaatcattc gtgcgtggtc accgttttta
      961 atcttaacgg tgttggtcac catctggacc atgaagccgt ttaaagcgtt atttgctccg
     1021 ggcggcgcgt tttattcact ggtgattaat ttccagatcc ctcatttgca tcaacaagtc
     1081 ttgaaagcgg cccccattgt cgcccagccg acgccaatgg acgcggtgtt taaattcgac
     1141 cccctctctg ctggtggcac cgctattttt attgcggcga ttatctcgat cttcatcctc
     1201 ggcgtgggga tcaagaaagg tattggtgtc tttgccgaaa cgctaattag tctgaagtgg
     1261 ccgatattgt cgattggcat ggtgctggcg ttcgccttcg tgaccaacta ttccggtatg
     1321 tccaccacgc tggcgctggt actggctggt acaggcgtga tgttcccatt cttctcaccg
     1381 ttcctcggct ggctgggcgt attccttacc ggctcggaca cttcttcaaa cgccctgttt
     1441 ggttcactgc aatcgaccac ggcgcagcaa atcaacgtct ctgacaccct gctggtggca
     1501 gcaaacacca gcggcggcgt aactggcaag atgatctccc cgcaatctat cgccgtggcc
     1561 tgcgccgcga cgggcatggt ggggcgagaa tctgaactgt tccgctacac cgtgaagcac
     1621 agtctgattt ttgccagcgt tatcggcatt atcacgctgt tgcaggcgta tgtgtttacc
     1681 gggatgttag tttcgtaagt caataatgcc ggatgatcgt taatatcatc cggcttcgtt
     1741 tacattattc tgtcttatta atggaaaatt atttcccgct ttattatcca tgcggcaata
     1801 atgttttgcg ttcagataac atgctaaata attaagagcg tgagaacatc tattttttat
     1861 cacgcaaaac aagtctgtat taattatgtt cgcttgatga gcaaactccg ttctatctgt
     1921 tgcttttgca atcttaatcg ttaaaactac caacaaat
//
LOCUS       DAJOTB010000312         1947 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJOTB010000312 DAJOTB010000000
VERSION     DAJOTB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.317"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..582
                     /gene="tetC"
                     /locus_tag="OHN28_004927"
     CDS             <1..582
                     /gene="tetC"
                     /locus_tag="OHN28_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCQ2300162.1"
                     /translation="NHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKALVTKG
                     AFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKN
                     LIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFM
                     HIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(695..1900)
                     /gene="tet(B)"
                     /locus_tag="OHN28_004928"
     CDS             complement(695..1900)
                     /gene="tet(B)"
                     /locus_tag="OHN28_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCQ2300163.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          677 a          417 c          350 g          503 t
ORIGIN      
        1 aatcatcaac aagaaaattt taagagtacc tatcaatcac tggttaactc agcacgaata
       61 ttgtttgttg aaaaaggcta tcaagctgtt tcaatagatg agatctcggg aaaagcgttg
      121 gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa aacaattact cagtgcctgt
      181 tataagcagc aattaattat gattgatgcc tacatcacaa caaaaactga tttaacaaat
      241 ggttggtctg ccttagaaag tatatttgaa cattatcttg attatattat tgataataat
      301 aaaaacctta tccctatcca agaagtgatg cctatcattg gttggaatga acttgaaaaa
      361 attagccttg aatacattac tggtaaggta aacgccattg tcagcaaatt gatccaagag
      421 aaccaactta aagcttatga tgatgatgtg cttaaaaact tactcaatgg ctggtttatg
      481 catatcgcaa tacatgcgaa aaacctaaaa gagcttgccg ataaaaaagg ccaatttatt
      541 gctatttacc gcggcttttt attgagcttg aaagataaat aaaatagata ggttttattt
      601 gaagctaaat cttctttatc gtaaaaaatg ccctcttggg ttatcaagag ggtcattata
      661 tttcgcggaa taacatcatt tggtgacgaa ataactaagc acttgtctcc tgtttactcc
      721 cctgagcttg aggggttaac atgaaggtca tcgatagcag gataataata cagtaaaacg
      781 ctaaaccaat aatccaaatc cagccatccc aaattggtag tgaatgatta taaataacag
      841 caaacagtaa tgggccaata acaccggttg cattggtaag gctcaccaat aatccctgta
      901 aagcaccttg ctgatgactc tttgtttgga tagacatcac tccctgtaat gcaggtaaag
      961 cgatcccacc accagccaat aaaattaaaa cagggaaaac taaccaacct tcagatataa
     1021 acgctaaaaa ggcaaatgca ctactatctg caataaatcc gagcagtact gccgtttttt
     1081 cgccccattt agtggctatt cttcctgcca caaaggcttg gaatactgag tgtaaaagac
     1141 caagacccgc taatgaaaag ccaaccatca tgctattcca tccaaaacga ttttcggtaa
     1201 atagcaccca caccgttgcg ggaatttggc ctatcaattg cgctgaaaaa taaataatca
     1261 acaaaatggg catcgtttta aataaagtga tgtataccga attcgattgc gtctcaaccc
     1321 ctacttcggt atctgtatta tcacgtgtat ttttggtttc acggaaccaa aacataacca
     1381 caaggaaagc gacaatattt agcaacgcag cgataaaaaa gggactatgc ggtgaaatct
     1441 ctcctgcaaa accaccaata ataggccccg ctattaaacc aagcccaaaa cttgccccta
     1501 accaaccgaa ccacttcacg cgttgagaag ctgaggtggt atcggcaatg accgatgccg
     1561 cgacagcccc agtagctcct gtgatccctg aaagcaaacg gcctaaatac agcatccaaa
     1621 gcgcacttga aaaagccagc aataagtaat ccagcgatgc gcctattaat gacaacaaca
     1681 gcactgggcg ccgaccaaat cggtcagaca tttttccaag ccaaggagca aagataacct
     1741 gcattaacgc ataaagtgca agcaatacgc caaagtggtt agcgatatct tccgaagcaa
     1801 taaattcacg taataacgtt ggcaagactg gcatgataag gccaatcccc atggcatcga
     1861 gtaacgtaat taccaatgcg atctttgtcg aactattcat ttcacttttc tctatcactg
     1921 atagggagtg gtaaaataac tctatca
//
LOCUS       DAJOTB010000313         1909 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJOTB010000313 DAJOTB010000000
VERSION     DAJOTB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.318"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1294..1896
                     /gene="fimB"
                     /locus_tag="OHN28_004929"
     CDS             1294..1896
                     /gene="fimB"
                     /locus_tag="OHN28_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCQ2300164.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          585 a          347 c          397 g          580 t
ORIGIN      
        1 taaataaaca gagaaccaga ttaaatacct gagtataaaa tctcttctga tgtttaattg
       61 atttgaatgt tcgtaagcta tatcacttac tcaatccatt ttacccagag tcataaaata
      121 gagtgtctgt tgatctcatt ttcatattcc tttcagactg aagtgcgttt tcatcacccg
      181 atgagaaaga actgaagtga tgtttgccat agattgcgag atgccctgtc tcattgacta
      241 tcgcggtgca cctttatacc tgttatacca gatcaaaaat cacgcaatcc atacaacaaa
      301 acgagatttg caattcgtgt cacaaaatat gtcgatcttt ttctaagagg aagatgccat
      361 gtgaagccag acgaacactt gcggtggtct tcaaaaacta aatgatttct tttaacagtt
      421 tgttttataa aaaatttatt tagggtctaa acaagggaag ctttgcaagc taactcagtg
      481 agcgtggtga aaacaagtgt ttacccgcca tcaggatgat cataattctc atcatgaaat
      541 atgtttcctg gttttggctt gtaagtggtc acccctgaag tcgatctgga aaggcttgta
      601 gatgttggtg ttttcaggat gatgtttcag atagtttgtt tgccgtatcg cccggcgaat
      661 agctgtgatt gaggaagatt taagtcgcag tgaccaaagc catatttacc aacgaatgta
      721 gatgaaaaag cgtctcctgc gttcccccat atctccagga aatacacagt ctgaaaaagg
      781 aatgtaacaa tctcatggcg taagctgacg aaccaggcag gaataatcgc tagggaccta
      841 agcattagca tgataatagc cactaagaaa ttactgcgtt ccatgaaata gccattttgt
      901 ggcaaatgca gttgactaat aatgtcatat gtgagacggc tagttgaaca aatattaaat
      961 tttgctgaat tttttatgtt gattttactt gttacagaac atatcacatg atatatagat
     1021 aagattagtt gcattaatga tgagggttat tattcgattc gtatcagatt gataaatata
     1081 taaaggtaca tagcatgcaa gagcatggcg tttgtatggc aaagttatta taagtaacta
     1141 tcgctactcc atttaagttc actcagaaga actggtccac ttacgttagt tattaagcaa
     1201 acgttcgctt ttataaacat aatcaggatt aaaatgttgg attattgcta acccagcaca
     1261 gctagtgcgc gtctgtaatt ataagggaaa acgatgaaga ataaggctga taacaaaaaa
     1321 aggaacttcc tgacccatag tgaaatcgaa tcactcctta aagcagcaaa taccgggcct
     1381 catgctgcac gtaattattg tctgacatta ctttgtttta ttcatggttt ccgggcgagt
     1441 gaaatttgcc gattgaggat ttcggatatt gatcttaagg caaagtgtat atatatccat
     1501 cgattaaaaa aaggcttttc aacaacgcac ccgctattga acaaagaaat tcaggcttta
     1561 aaaaactggt tgagtatccg tacttcttat ccgcatgctg agagcgagtg ggtattttta
     1621 tcacgtaagg ggaatccact ttctcggcaa cagttttacc atattatctc gacttccggt
     1681 ggtaatgccg ggttgtcact ggagattcat ccgcacatgt tacgccattc gtgtggtttt
     1741 gctttggcga atatgggaat agatacgcga cttatccagg attatcttgg gcatcgcaat
     1801 attcgtcata ctgtctggta taccgccagc aatgcagggc gtttttacgg catctgggat
     1861 agagccagag gacgacagcg tcacgctgtt ttatagagat agttgagat
//
LOCUS       DAJOTB010000314         1888 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJOTB010000314 DAJOTB010000000
VERSION     DAJOTB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.319"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            129..383
                     /gene="bssS"
                     /locus_tag="OHN28_004930"
     CDS             129..383
                     /gene="bssS"
                     /locus_tag="OHN28_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCQ2300165.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            498..1616
                     /gene="solA"
                     /locus_tag="OHN28_004931"
     CDS             498..1616
                     /gene="solA"
                     /locus_tag="OHN28_004931"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCQ2300166.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1664..1777
                     /locus_tag="OHN28_004932"
     CDS             1664..1777
                     /locus_tag="OHN28_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCQ2300167.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          488 a          460 c          445 g          495 t
ORIGIN      
        1 aaatttatct gttccccctt taaaaattcg gtgaataccc ttacttattg gtatactgaa
       61 gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac acgaattagg
      121 gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta gggtgggaca
      181 tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag accccaaata
      241 agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact gatgttgcca
      301 gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt gatttccagg
      361 taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc acctcaccgg
      421 tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc ttaaaccatg
      481 agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt tccgtaggcg
      541 ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc gacgcccata
      601 tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc catgcttatg
      661 gtgaaggcga aaagtatgtc ccgctggtcc tccacgcgca aatgctgtgg gatgaacttt
      721 cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac ctcggtccgg
      781 ctgactccgc atttctcgcc aacgtcgccc acagcgccga ccagtggcaa ctcaacgttg
      841 aaaagctcga cgcgcaaggg attatggccc gctggccaga aatacgcgtc ccggacaact
      901 acatcggttt atttgaaact gattccggtt ttttgcgcag cgaactggcg attaaaacct
      961 ggatccaact ggcgaaggaa gcgggctgtg cgcagctgtt caactgcccg gtcaccgcaa
     1021 ttcgtcatga cgatgatggc gtaactattg aaacggcaga cggtgagtat caggcgaaaa
     1081 aagcgattgt ctgcgcggga acatgggtaa aagacctact cccggagctg cctgtccagc
     1141 ccgtacgcaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg aagaataaat
     1201 tcccggcatt taccggtgag ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa
     1261 acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca gcggatgaac
     1321 gtgttccgtt tgcggaagtg gccagcgatg gttcggaagc cttcccgttc ttgcgcaatg
     1381 tgttgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat aattcgcctg
     1441 acgaagattt tattatcgat accctacccg gccacgataa tacactgctc attaccggcc
     1501 tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc gattttgcgc
     1561 aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc caataatcat
     1621 cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc gactgttgca
     1681 ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct ggggattgtt
     1741 ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga cataaactga
     1801 caaatattta atctcccttt catttagcgt gaaaaattga atgagattct tcctgcctct
     1861 acagcactca ctagaccttc cagatgct
//
LOCUS       DAJOTB010000315         1862 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJOTB010000315 DAJOTB010000000
VERSION     DAJOTB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.322"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1163
                     /locus_tag="OHN28_004933"
     CDS             <1..1163
                     /locus_tag="OHN28_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2300168.1"
                     /translation="ALTDIQIKRAKPQNKPYTLNDGQGLSLLINPDGSKGWRFRFRFS
                     GKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKALRLAQKLSTENSFEAI
                     CREWHTNKADRWTVAYREEIIKTFEQDVFPFIGKRPISEIKPLELLEVLRRIEKRGAL
                     EKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRDL
                     EAYTGSIITKNATKIVMLTGVRTQEMRFATWEEVDLEKGIWEIPAERMKMRRPHIVPL
                     STQVVDLFKQLKPITGHYPYIFIGRNNRSKPISKESVSQVIELIGYKGRATGHGFRHT
                     MSTILHEQGFDSAWIEIQLAHVDKNRIRGTYNHAQYLEHRKKMMQWYSDKLY"
     gene            1176..>1862
                     /locus_tag="OHN28_004934"
     CDS             1176..>1862
                     /locus_tag="OHN28_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCQ2300169.1"
                     /translation="MDLLNKHIASILRAKDEESLAIFVGAGVSKSSETKTIKMPSWGD
                     LIDALISDLNIKDESDYLKIAQLYYLTFGEHLYYKRIKDFFPENVPHSKIHDLIFKLN
                     PHSVITTNWDTLLEAAINAKSYFYNIISSDKDLMKSYLGKKLIKMHGDFKNHNIVFKE
                     DDYLNYSFNFPLIENYVKSVISTHTVLFLGYSYNDIDLKQIIKWTQNHSSVRPPMYLV
                     VFEDIPAQRKY"
BASE COUNT          622 a          365 c          361 g          514 t
ORIGIN      
        1 tggctctcac cgatatacag atcaaacgtg caaaaccaca aaacaagcca tacacattga
       61 acgatggaca aggtctgtca ttgcttatca atcccgatgg ctcgaaaggc tggcgtttcc
      121 gtttccggtt ttcagggaaa gcgcggttaa tgtcatttgg cagctacgat ttagtaagcc
      181 tcgcagaagc acgtgagaag cgtgatatcg cccgtaagca ggttgctaat ggcattgacc
      241 cggtagagga acgcaaagct ttaagactcg cccaaaagct atcaacagaa aattctttcg
      301 aagcaatatg tcgagaatgg cataccaaca aagctgaccg ctggactgtg gcctatcgag
      361 aagaaatcat taagacattc gagcaagatg tcttcccgtt cattggtaaa cgtcctatca
      421 gtgaaatcaa accattagaa ctgcttgaag tattacgacg aatcgaaaaa cgtggagcac
      481 tagagaaaac acgcaaggtg cgtcaaagat gcggtgaggt ttatcgctat gcaatcataa
      541 ctggccgtgc tgagtacaat cctgcacctg atttagctat cgctctggcc gttcccaagc
      601 aaaaacacca tccattttta tccgctgaag agttgcctca ttttattcga gatcttgaag
      661 cgtataccgg tagcatcatc accaaaaatg ctacgaagat agtcatgctg actggtgtaa
      721 gaacgcagga gatgcgcttt gctacgtggg aagaagtaga cctcgaaaaa ggtatatggg
      781 agataccagc ggaacgtatg aaaatgcgta gacctcacat tgttccttta tctactcagg
      841 tagttgacct tttcaaacag ctcaaaccta ttaccggcca ttacccttac atctttattg
      901 gcaggaacaa ccgcagcaag ccaatctcaa aagaaagtgt ttcacaagtg attgagttaa
      961 ttggctacaa aggccgtgct acaggtcacg gttttcggca taccatgtcg acaatattgc
     1021 acgaacaagg gtttgatagc gcatggattg aaatacaatt ggcacatgtt gataaaaaca
     1081 gaatccgagg gacttacaat catgctcaat atcttgaaca tagaaaaaaa atgatgcaat
     1141 ggtattcaga taaattatat tgagaggtat atacaatgga tttgctaaac aaacatatag
     1201 ctagtatttt aagagcaaaa gatgaggagt ctttagctat ttttgtaggg gctggtgttt
     1261 caaaatcctc tgaaacaaaa actatcaaaa tgccttcatg gggggactta attgacgctc
     1321 tcatttcaga tctcaatatc aaagatgaat cagattattt aaaaatagca cagctttatt
     1381 acctcacatt tggtgagcat ctttactaca aaagaatcaa agatttcttt cctgaaaatg
     1441 ttccccattc aaagattcac gatctaatat ttaaactgaa tcctcactct gttatcacaa
     1501 cgaattggga cactttgctt gaagcagcta taaatgccaa atcttacttt tataatataa
     1561 taagcagtga caaagatttg atgaaatctt accttggcaa aaaattgata aaaatgcatg
     1621 gtgattttaa aaatcacaac atcgtcttta aagaagatga ttacttaaat tatagcttca
     1681 atttcccctt aattgaaaat tatgttaaaa gtgttatttc cacccatact gtgctatttt
     1741 tggggtattc atataatgat atagatctaa agcaaataat taaatggact cagaatcatt
     1801 catctgttag accccctatg tatttagtag tattcgaaga tattcctgct caaagaaaat
     1861 at
//
LOCUS       DAJOTB010000316         1851 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJOTB010000316 DAJOTB010000000
VERSION     DAJOTB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.323"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..332
                     /locus_tag="OHN28_004935"
     CDS             <1..332
                     /locus_tag="OHN28_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300170.1"
                     /translation="RNVGTGANQIPDMGSFTLSVSGTGYQKLPSGFILQWGSIGAPGI
                     AQDVVTHFPIAFPNRCLRVLVSQDYTPDSGAVGYIACAGFSPDPVKFISRASTPGLGA
                     SFLALGC"
     gene            354..794
                     /locus_tag="OHN28_004936"
     CDS             354..794
                     /locus_tag="OHN28_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2300171.1"
                     /translation="MNYIYSATTNSFYPLEMKEDYTQADSWPDDAVEVDEQVYIEFSG
                     LPPKGKIRIAGENGFPAWSEIPPPTHEEQIAAAELEKQQLINQANDYMNSKQWPGKAA
                     IGRLKGEELAQYNLWLDYLDALEMVDTSSAPDIEWPTPPAVQAR"
     gene            complement(766..1368)
                     /locus_tag="OHN28_004937"
     CDS             complement(766..1368)
                     /locus_tag="OHN28_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2300172.1"
                     /translation="MHKAILNSELIATKAGDVTVYNYDGETREYISTSNEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSADFNSWEYVPDHRGETVYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1368..>1851)
                     /locus_tag="OHN28_004938"
     CDS             complement(1368..>1851)
                     /locus_tag="OHN28_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCQ2300173.1"
                     /translation="SALPVGIPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNK
                     LPDLRGEFIRGWDDGRGIDTGRSILSIQGYATEDHAHGLPSRSTIVTDATINFYFDES
                     WVNSGTDIIKRGNTNDAGLPAPDYGTFKTYKQSVAGLGAAASETRPRNIAFNYIVRAA
                     "
BASE COUNT          470 a          433 c          433 g          515 t
ORIGIN      
        1 aaaggaatgt ggggacaggg gcgaatcaga tacctgatat gggtagcttc acgctttctg
       61 tttcaggtac tggatatcaa aaattaccat ctggttttat tcttcagtgg ggctcaatcg
      121 gcgctccagg cattgcacag gatgtggtaa cccatttccc gattgcattt ccgaacagat
      181 gtctgcgtgt tttggtctca caagactaca caccagatag cggggctgtt ggttatattg
      241 cctgtgcagg ttttagtccc gacccggtta aatttatatc cagagccagt actccaggtc
      301 tcggcgcttc atttttagcg ttaggctgtt aatttagcta tatggaatga gaaatgaatt
      361 atatatattc cgcgactaca aactctttct atccgttaga gatgaaagag gattacactc
      421 aagctgactc atggccagat gatgctgttg aagttgacga gcaggtgtat attgagtttt
      481 ccggattacc gccgaaagga aaaatccgta tcgctggaga aaatggtttt cctgcatggt
      541 ctgaaattcc accaccaaca catgaggaac agattgctgc agccgaactg gaaaagcagc
      601 aattgattaa tcaggccaac gattatatga acagtaaaca atggcctggt aaagcggcta
      661 ttggtcgtct gaaaggtgag gaactggcgc aatataattt gtggctggat tacctggacg
      721 cactggaaat ggtcgatact tccagtgcac cagatattga atggcctacg cctccggcag
      781 ttcaggccag atgacatccg gcgcggtgct ggtatctgtt gccgtcaccg cgtcaatgta
      841 atccagcaca gcgttaagcc gggttgtttc tgcctgcgtc agtttacgtc cggcctgcaa
      901 tttcagttga atcagactga tggaagccat tgcagtatca atcagcgact ggcgctgtgc
      961 ttctgctgca tctactgcgg cgctatgctg tgcctcggta tccgtcaccc atttctcacc
     1021 atcccattta tcatatggcg ttaatggggc gatagtggtt gtattttcag ggtaatcacc
     1081 cggagcggtg atttcttttg attctcctgt ttcggtgcta tagaccgttt caccgcgatg
     1141 gtctggcaca tattcccatg agttaaaatc tgcagaacgg cagattgcat aaccagcctt
     1201 atgtgtgcca ggggcatcta aacaggaaca tgccggaatg ccgacaccaa cggcaagata
     1261 ttcatttgaa gtggaaatat attcccgtgt ttcaccatca tagttataaa cggtaacatc
     1321 ccctgccttt gttgcaatga gctcactatt taatattgct ttatgcatca ggctgccctc
     1381 acgatatagt taaatgcaat attacgcgga cgcgtttctg aggctgcggc acctaaacca
     1441 gccactgatt gtttatatgt tttaaaggtt ccataatccg gtgctggtaa tccagcatcg
     1501 tttgtgtttc ctcttttgat aatgtcagtg ccactattta cccagctttc atcaaaatag
     1561 aaattaatcg ttgcatcagt cacaatcgtg gatcttgacg gtaatccatg agcatgatcc
     1621 tccgttgcat acccctgaat acttaaaata gagcgacctg tatcaatccc ccgcccgtca
     1681 tcccagccac gaataaactc accacgtaaa tcaggcaatt tatttgtcgg gtaagccttt
     1741 gccagttccg ggtattcttc agccgaaaaa gcagccccgt tgcatttcag ccagcctgtt
     1801 ggcggcgtag ctgaaggcca aggaactggt atgccaacag gcaacgcaga a
//
LOCUS       DAJOTB010000317         1824 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJOTB010000317 DAJOTB010000000
VERSION     DAJOTB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.324"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            556..1503
                     /locus_tag="OHN28_004939"
     CDS             556..1503
                     /locus_tag="OHN28_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HCQ2300174.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGAFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISTKNGAGIESYNFVTTAGLKYYF"
BASE COUNT          564 a          344 c          396 g          520 t
ORIGIN      
        1 aaaggaggga tttataaacc agcaactttt tttgtaggtg gagccacggt tgatttctga
       61 tatcaaccac ctgagtttat tttcgtcgaa tggtaatgtc ttgtcgcaca aacgctatct
      121 gaataattaa ctgtaatggc gttattggta tatttgccga cattaacgct aataaagacc
      181 ctgattgctt cataatcgca ggatttggcc attttgtctc cctttagata agagttaaca
      241 aaataacccc aggaagaagg taccggtttc cacgttacag acgttcccgg cataaagtgt
      301 ccgaccaggg ccttattcgc agttaccagc gccctgtatg ccctgatatt ttctggaaca
      361 tggatgaccg gggcagtaat cataccgggt ttgactgact cccctgatat tctgacataa
      421 aagggaataa ataaagcgac atgtggccac attgtaaaca aacgttaaac aaaacagcat
      481 ttttcagttg actactacga gtaaataaaa aaatatttat caaaaacaaa cgagtggaat
      541 ggagttaact tgtctatgta cttaaagatt cttgctactg cactctcagc tcctgtagcg
      601 tttgctgcac tggcttctga tactgggctt tcttttacac cggagaaaat cagtacggag
      661 attgattttg gcactttaag tggcaaggcc aaagaacggg tttatctgcc tgaagaaaaa
      721 gggcgcaaag ccagccagct ggactggaaa tacagtaatg cgccaattgt caagggggca
      781 tttaactggg atctgttacc cagagtatct gtcggtgcct cagggtggac aacattagct
      841 ggtcgtggag gcaatatggt ggacagggac tggctggata cgagcaatcc cggaacctgg
      901 actgatgaga gtaaacatcc gaatacccgt ctaaattttg ctaatgaatt cgacctgaat
      961 attaagggat ggctcctgaa tcaaccagac tatcagttag gtcttatggc tggttaccag
     1021 gaaaatcgtt atagctttac agcaaaagga ggctcttata tctatagtag tgagggaggc
     1081 ttccgggatg aaacgggagc atttccagat ggtgaaagag ctataggcta caaacaacac
     1141 tttaaaatgc cctatatagg gttaaccggt aattatcggt atgatagctt tgagttcggg
     1201 gggagtttca aatatagcgg atgggtgaag gcatcggata atgatgagca ttacaaccct
     1261 gagaaaagga tcacctatcg tagcgatgta aataatcaga actattactc tgtctcacta
     1321 catgctggtt attatattac acctgcagct aaagtttatg tcgaaggaac atggaaccgt
     1381 attactaata agaaaggtga tacttctctg tactccagaa atttgaatat atccaccaaa
     1441 aatggtgcag gcattgaaag ctataacttt gtgaccacag ctggtctgaa gtattatttt
     1501 taacctgaag catgcctgtt tttaactttt tcatgacact ttaaatatga taaacaaaag
     1561 gccagcactt ctgatggtgg tcttttgatt ttttataata tgtggagggt atatgtgctc
     1621 aacgataaaa gtgtttctgt taactcttgt atttttaaag atgctgccct gccactcttc
     1681 atgcataacg acacagtgtt atcgacataa aaataattga taagcgatgt taacaatatg
     1741 aactttatta taattctatt actcgtcaat aataagaaga ccatccaata aaaacacacg
     1801 caataacctg aaggattctg ccgt
//
LOCUS       DAJOTB010000318         1802 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJOTB010000318 DAJOTB010000000
VERSION     DAJOTB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.325"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            158..661
                     /locus_tag="OHN28_004940"
     CDS             158..661
                     /locus_tag="OHN28_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCQ2300175.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(739..990)
                     /locus_tag="OHN28_004941"
     CDS             complement(739..990)
                     /locus_tag="OHN28_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCQ2300176.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="OHN28_004942"
     CDS             complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="OHN28_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCQ2300177.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1455..1778
                     /locus_tag="OHN28_004943"
     CDS             1455..1778
                     /locus_tag="OHN28_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCQ2300178.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          462 a          430 c          400 g          510 t
ORIGIN      
        1 tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac
       61 atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc
      121 ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct
      181 caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag
      241 taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca
      301 gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc
      361 catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca
      421 aaaaacgatg gaagaatacg agcaacgttc aagcacgctg gcacagttag ccgatgaagc
      481 gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca
      541 gcatgatggc ctgttactgc aaaccattct tgatgaagtg cgcagtgcga aacttgctgg
      601 tatgtgtcct gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctacattg
      661 atcatcatcg gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcattt
      721 ttttgaagca gcggtttttt aatgggattc gccctgtggg gtaaacttga gttcaatcaa
      781 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt
      841 aaagtcgata ttcttcagct ggctggacat tttttcgcga acttcaacgg gcgcgatcac
      901 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
      961 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     1021 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca gaacacaaaa
     1081 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     1141 cgtcttgcaa tagttattga acatactttt caggatcttg cgcagtttca tcgcggtact
     1201 ccgaccattt gttatacagg tgttattgtc tttgcgttta taatatgaca accatcacaa
     1261 aaatcaatct ttatgtgatg taaatcacat aaatacccct ttaatgttat aaaaatgata
     1321 atcaaaaaag cagccctcca atttctgaca catacagatg gcaagaaata gcgcctgcca
     1381 ggcgtctttt ccggccattg tcgcagcgct gtaacgcgta aaatactgct ttcttttact
     1441 cttctggctg gaccatgaga cttctgattc tgactctttc cttaataacg ctgacggggt
     1501 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgttcggt
     1561 tggtttatga tcaggctttt ttgcagcatg cccgtaccga tcgttatgtc agtcgcggga
     1621 tcgccgatcg cgcatgccag caggcaggct atacccacgc tatccccttt ggtcagccag
     1681 taggcaactg tagcctcttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
     1741 atcagtgcca tcattccgcc ttccctgtct ttctctaaac gttttaatca gcgaggggga
     1801 tc
//
LOCUS       DAJOTB010000319         1762 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJOTB010000319 DAJOTB010000000
VERSION     DAJOTB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.327"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(20..679)
                     /locus_tag="OHN28_004944"
     CDS             complement(20..679)
                     /locus_tag="OHN28_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2300179.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGK
                     STLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELN
                     ERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
                     KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKD
                     L"
     gene            complement(676..1452)
                     /locus_tag="OHN28_004945"
     CDS             complement(676..1452)
                     /locus_tag="OHN28_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2300180.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
BASE COUNT          558 a          369 c          314 g          521 t
ORIGIN      
        1 taatattgga atgtaaccac tataggtctt ttttgtaatc agcaatggct tccgtaacat
       61 ttttataaaa tttaccttgc ccctctttgt taagataaat agcaacatca caatactcct
      121 ttaaagctcg ttcactatgt gacaccatta ttaaatgaga tttttctctt attttatcga
      181 atatatctga acatttcttt ttaaactttg cgtctccaac agcagtgatt tcatcaataa
      241 gataatagtc gaatttaaaa gccatactta atccaaaagc taaccttgac ctcatgccag
      301 aagaataagt tttaatgggc atatcgaaat attttccgag ttcggaaaac tcttcaacaa
      361 aatcaaccct ctcatttaac tcatcgcgtt ttgcatatag tcgggcaaca aatttaacat
      421 tttcacgacc ggttaaactt ccttgaaatc caccagccaa tccaacgggc catgaaattt
      481 tatgttctgt aataatgttc ccactatcag gacgatctat accaccaatt atccgaagta
      541 aagttgattt accggctcca ttttgaccaa tcagggcaat gttatagcct ttaggaaaaa
      601 taatattcaa atttttaaaa acataatgcc gcccagtggg cgtgcgataa gacttcgtca
      661 aattctcaat cttaatcatg atgtcagcat tgcctcttca cgcgttcggt ataatgctag
      721 gccgatgaac agggtgacca gagtaaacat tgcaaggtag ttcagactca cgccttcact
      781 gatatagcca ggcataactg cctcgcggct taactccaca acatgcacta atgggttcca
      841 gagtagatat gaccagtatt gctttggaat cgagtgtaga gggaacatga tgcaggagat
      901 gaaatacagc ggcttaagca gtatcggcag gaccttttgc atttcaggaa aggttttacc
      961 aacgaccata aatattaagc cgacgccaca tgaaagaatg attaacaaac tccaggtgag
     1021 cacaagttgt aaaaagtttg taatttcgaa atattcgcct gtcatccaga cgataagcat
     1081 gagcaaaata taaacagcaa cgtaaatcag tgtctcaagc agtgcacgtg caatgatcgt
     1141 atcgatgggt tttactggtc gataattaaa cagtccctgg ttcgcttcaa tagcaccaat
     1201 agaacgtttg ctaatactac taaagataaa aaagggaatc aggccattaa gtaaaaacac
     1261 cgggaacgag atgtctggca tagtgcggtg cataacgtaa cccaaaattc ccaacagtat
     1321 cagcaaatgc gcggaaggtt cgagaatcgc ccacaaatat cccagacgga acttaccaaa
     1381 gcgtgttcgt atttctcgta gaaataatgc ctctacggtg actttctgga cttcaaatcc
     1441 acttcttgcc atttgatgat gtgatcctaa tctcttcagg tacgctaccg cccttggctt
     1501 aacagctacc aatacactgg aggtaatgct tatttgtaac tggataaaat ttactcagac
     1561 ggtttgacct ttttaatagc cgcaaaaaat ttcagcagaa attatttccg cagggataaa
     1621 tagatctatt tccgtctcgt ttcttctaat ccattcttca ggcatgaatg tattcggtct
     1681 tctggtaatt tcagaaaact ggccgtttta gaatgtatag ttaatttctc aggaaattat
     1741 ttttgttatt actgttaact ac
//
LOCUS       DAJOTB010000320         1709 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJOTB010000320 DAJOTB010000000
VERSION     DAJOTB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.328"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(84..209)
                     /locus_tag="OHN28_004946"
     CDS             complement(84..209)
                     /locus_tag="OHN28_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300181.1"
                     /translation="MEIKVQRPSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
     gene            complement(262..666)
                     /locus_tag="OHN28_004947"
     CDS             complement(262..666)
                     /locus_tag="OHN28_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCQ2300182.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(887..1618)
                     /gene="bluR"
                     /locus_tag="OHN28_004948"
     CDS             complement(887..1618)
                     /gene="bluR"
                     /locus_tag="OHN28_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCQ2300183.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          467 a          370 c          358 g          514 t
ORIGIN      
        1 tttctgcatg tgttaaaaac ggtaacgggc gtataacacg cccgttgttg ttataaatat
       61 gttaaccatt ataaaataac gtatcaaaag tcaagtgaac acatttcaaa tatcaatttg
      121 atcgtattgg tctggtgatt atttatggga agcaataaaa agacagtatt aatcatccat
      181 agggatggtc tctgcacttt tatttccatt atgctaatgc cttactgaat tatgaagcat
      241 ttcttaagct cccaacttta gctagattaa tggtttatta ttttctacat cttcaatata
      301 taaaagcgta ttatcaatgg cgtagtaact gcgtttgtta tgattaacat cagtaaccca
      361 ccggaaaacg cctgcgcctg ccagtgttga acagtattcc cgaaatgtag attttccgcg
      421 aatatgaagc aatgcggcct cttttatttt agctgggttc ttggtcgtac taacttttaa
      481 caggttcctg gttcctctta ataacaaaac cgtgtcatcg tgagtaataa ttctgatgtt
      541 atccgtagcc agataataaa tgtaatgtgc aatacggtga tgttttaatt ctgaataaaa
      601 ccaggagaag ttttgctctt ttctcacttg ctcaaacatc ttttgaaaaa caacgacctg
      661 atccatcagg ataataacct cttgttaggt gtgagactgc gtagtgtgca cgatcggttt
      721 taccacttca atctggtctg tcctttggct gtgatatgta caaagtgtca aagggggatt
      781 acctgaattc tcccggtgag cattttgcaa cggaccagct ccgctacaaa cgctgttgtg
      841 ggttcagatt ataacattct gtctaagggg cgggataaag gtgaatttag ggggcatgaa
      901 agatgacttt ataaccttgc tcaccccagt gttgtaaaag ttcgttttgc cttctcgttg
      961 gtgccatgcc tgtccagaca atcaatgttt gcgttgggaa cagttccggg cgcggcgatt
     1021 caatgggatc agcaagaaca gaaatgtgcc atcctgacaa tgataaacgc catgcttcca
     1081 gccacaatcg tgctctctct tcaacattcc acgccatcag aatggcctct ttaccgggct
     1141 tacggcgcat ttcgaaaagt gaggccgctg cgtattcaat taatgcgccg tcaaacatac
     1201 tgctcataat gcgggaggtg ttgtgatcaa gcacgagacg ctggcgaaca ggaaggtaaa
     1261 catgattaat caattgatct actgggtact ctcgacccag tgaaataatt cgcgcgcgta
     1321 gtttggctgg attagccata cgaagaattg acatcatctc ttcttgcagg cggctccagt
     1381 catcttgcgt atcctggcgg gtagtttcca gtaatgcttt aactttgcct acaggaacgc
     1441 cattacttat ccaacgcttg atctcttcga tgcgttgtat gtcttcttca tcaaagagtc
     1501 ggtgtccgcc ttcactgcgt tgtggtttta acaaaccgta gcggcgttgc caggcccgga
     1561 gagtgacagg attaatcccg caacgttcag caacatcacc aatgctgtaa taagccacaa
     1621 ttcctccttg cggtcataaa tctccgtcgc ctctacacga cccaataata ctttgtacaa
     1681 tatacgctaa aattgtacaa agtataaat
//
LOCUS       DAJOTB010000321         1704 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJOTB010000321 DAJOTB010000000
VERSION     DAJOTB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.329"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            3..626
                     /gene="tetR(B)"
                     /locus_tag="OHN28_004949"
     CDS             3..626
                     /gene="tetR(B)"
                     /locus_tag="OHN28_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCQ2300184.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(604..1290)
                     /locus_tag="OHN28_004950"
     CDS             complement(604..1290)
                     /locus_tag="OHN28_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCQ2300185.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1298..1684)
                     /locus_tag="OHN28_004951"
     CDS             complement(1298..1684)
                     /locus_tag="OHN28_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCQ2300186.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
BASE COUNT          514 a          371 c          320 g          499 t
ORIGIN      
        1 tgatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
       61 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      121 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      181 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      241 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      301 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      361 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      421 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      481 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      541 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      601 aacttaaatg tgaaagtggg tcttaaaagc agcataacct ttttccgtga tggtaacttc
      661 acggtaacca agatgtcgag ttaaccaccc tttagattca taaagcgaaa ataatgcggc
      721 tccaacgtac ccacctaaat ggaaacggcg ttcactccaa tctaaacacg cacaacagat
      781 tttacgtgaa tgtttggaag gaacgtcaat tcccatttca tgaaaatatt gaataccact
      841 taatgtgatc attgaaccat tttcagtgat ccattgctgt tgacaaaggg aatcatagat
      901 cttaacggca acttcgccag ctaaatgatc atagcaagta cgtgcttttc gtaaatgcac
      961 tggcgtggaa actttggcat gtacgccatg gtttaaggag atccccatca tactttccat
     1021 caattcagca atatcttttc ctgctagccg aaaataacga tgcttgcctt gagctactac
     1081 tgtgattagc tggcaatcta ataatttaga taaatgactg ctcgccgttg aagctgatat
     1141 attcgccaca gaacttagct cagtggccgt ccaagctcgc ccatccatca aagcactgag
     1201 tattttaact cgtgaaatgt cagacatagc cgcccctatc gcggctattg aggactcaaa
     1261 ggtaacctct tttcgtatta aattagccat cgcaagttca ctttattgcc caagggagcg
     1321 taacagatgc agccatacta tcattgtccg ttattaatat cagttggtta gcatggtcac
     1381 tgtattgcac taaaatatta atgttattct ccgccaatac tcgtgctatt tcgccaagtt
     1441 cccccggttt ttcctgtttt aacttacgaa ttaatggtgt ccggatcgca agtactaaca
     1501 gtccagcttg ctctagcgct attttagctt cctttccttg ttcaacaaga aaatgagcat
     1561 ggcattcatc accaaccgta aatatccctc ccccttccaa tccaatacct ttattaccta
     1621 atgtttttcc tagtaatgct aattgtccta tttgattatc taaaacaacg tgaacatcaa
     1681 acattatcct tgtccttttt aaat
//
LOCUS       DAJOTB010000322         1698 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJOTB010000322 DAJOTB010000000
VERSION     DAJOTB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.330"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            288..1694
                     /gene="gndA"
                     /locus_tag="OHN28_004952"
     CDS             288..1694
                     /gene="gndA"
                     /locus_tag="OHN28_004952"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCQ2300187.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQRAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          457 a          390 c          409 g          442 t
ORIGIN      
        1 taaaattaag tggagtagat ctttactata ctccccattt tgatgctatt cattatgcgc
       61 agcatgaaaa tagaagaata tttactaaag catttcgatg gcatataagg agtattacgc
      121 gctacatatt acggaaacca attctttctt ataaaaacta tagaaaaatt acatccgaac
      181 tggtaaagtg attaaataaa gtcctaattt tactactgaa atggtatctt tatttaaaga
      241 tctttgcttt atataccaat tttttaacgc cctgacagga gtaaacaatg tcaaagcaac
      301 agatcggcgt cgtcggtatg gcagtgatgg gacgcaacct tgcgctcaac atcgaaagcc
      361 gtggttatac cgtctctatt ttcaaccgtt cccgtgaaaa aacggaagaa gtgattgccg
      421 aaaatccagg caaaaaactg gttccttact atacggtgaa agagtttgtt gaatctctgg
      481 aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat gctgctattg
      541 attcccttaa gccgtacctc gataaaggtg acatcatcat tgatggtggt aacaccttct
      601 tccaggacac tattcgtcgt aatcgtgagc tttctgcaga aggctttaac ttcatcggta
      661 ctggtgtttc tggcggtgaa gaaggcgcgc tgaaaggacc ttccattatg cctggtggcc
      721 agaaagaagc ctatgaactg gttgcgccga tcctgaccaa aatcgccgca gtggctgaag
      781 acggggagcc atgcgttacc tatattggtg ccgatggcgc aggccactat gtgaagatgg
      841 ttcacaacgg tattgaatac ggtgatatgc agctgattgc tgaagcctat tctctgctta
      901 aaggtggcct gaacctcacc aacgaagaac tggcgcagac ctttaccgag tggaataacg
      961 gtgaactgag cagctacctg attgacatca ccaaagatat cttcaccaaa aaagatgaag
     1021 acggtaacta cctggttgat gtgattctgg atgaagctgc aaacaaaggt acgggtaaat
     1081 ggaccagcca gagcgcgctg gatctcggcg aaccgctgtc gctgattacc gagtctgtgt
     1141 ttgcacgtta tatctcttct ctgaaagatc agcgcgttgc cgcgtctaaa gttctctctg
     1201 gcccgcaagc gcagcgagca ggcgacaaag ctgagtttat cgagaaggtt cgtcgtgcgc
     1261 tgtatctggg caaaatcgtt tcttacgctc agggcttctc tcagttgcgt gctgcgtctg
     1321 aagaatacaa ctgggatctg aactacggcg aaatcgcgaa gattttccgt gctggctgca
     1381 tcatccgtgc gcagttcctg cagaaaatca ccgatgcata cgccgaaaat ccgcagatcg
     1441 ctaacctgct gctggctccg tactttaagc aaatcgccga tgactatcag caggcgctgc
     1501 gcgatgtcgt tgcttatgca gtacagaacg gtatcccggt tccgaccttc gccgctgcgg
     1561 ttgcctatta tgacagctac cgtgccgctg ttctgcctgc aaacctgatc caggcacagc
     1621 gtgactattt cggtgcacat acttataagc gcatcgataa agaaggtgtg ttccataccg
     1681 aatggctgga ttaatctg
//
LOCUS       DAJOTB010000323         1630 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJOTB010000323 DAJOTB010000000
VERSION     DAJOTB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.331"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..854)
                     /locus_tag="OHN28_004953"
     CDS             complement(<1..854)
                     /locus_tag="OHN28_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2300188.1"
                     /translation="MKLTKLVLENFRSVRERQEIDFAPVTLLLGPNSAGKTTILIALF
                     YLQQILDREQCDPIYLEAMGNRRIDGFRSLVHNGDLNNVITIGIGFDPEQTIGVEYKT
                     YLEELGDIFDIHFIKMKDIADETEKVYVEFKIAWSQLHRAAYIKNYAVSINNEFIGEL
                     SNDEYTKSSLVNRLNFTHPLLLPIDYNLWLESIDKEGLDTIASEFEISLNDLIPGLER
                     NKSGATERVKTVDYIFEEDKEKPMFPVFLKTKIGALPQLGRTVDTNLKVFDISEPVEY
                     FNRDIITRV"
     gene            complement(863..>1630)
                     /locus_tag="OHN28_004954"
     CDS             complement(863..>1630)
                     /locus_tag="OHN28_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCQ2300189.1"
                     /translation="LSVKKIQDIKIRLPSLKEQKRIAAILDKADAIRQKREQAIKLAD
                     DFLRAKFLEMFGTPANNIHRFPKGTIRDLVDSVNYGTSAKASIDSGEYPILRMGNITY
                     QGRWDFTDLKYLDLSVKEKDKYLVKEGDLLFNRTNSKELVGKTAVYEEDRPMAFAGYL
                     IRVRPNSIGNNYYISGYLNSIHGKITLMNMCKSIVGMANINAQELQNIEILIPPKHLQ
                     DEYEIIYKKIKKGLSIYDKSAMQLQLLASNLSNKYFM"
BASE COUNT          512 a          307 c          232 g          579 t
ORIGIN      
        1 actctggtga ttatatctcg attaaaatac tcaactggct cggatatatc aaaaactttc
       61 aaatttgtat caacagttct tcccagttga ggtaacgcac caattttcgt ctttaaaaaa
      121 acaggaaaca ttggtttttc tttatcttct tcaaatatat aatcaacagt ttttactcgc
      181 tctgttgcac cacttttatt acgttctaag ccaggaatta agtcatttaa agatatttca
      241 aattcactgg caatcgtatc taatccttcc ttatcaatag attcaagcca taaattataa
      301 tcaataggaa gaagcaacgg atgagtaaag ttcaatctat ttactaaaga tgatttagta
      361 tactcatcat tacttagctc tccaataaat tcattattta tagaaacagc atagttcttt
      421 atatatgctg ctctgtgcaa ttgtgaccat gcgattttaa actctacata aaccttttca
      481 gtttcatctg caatgtcttt catttttata aaatgaatat cgaaaatatc accaagttct
      541 tctaaatatg ttttatattc tacacctatt gtttgttcag gatcgaaacc gataccaata
      601 gtaattacat tattcagatc cccattatgg actagagatc taaatccatc tatgcgcctg
      661 ttccccatag cttctaaata tataggatca cactgctcac ggtctaatat ttgttgaaga
      721 taaaataatg caattaaaat agtagtcttg cccgcgctgt ttggccccaa tagtaatgtt
      781 acaggtgcaa aatcaatttc ctgccgctct ctaacagatc tgaaattttc aagtacaagt
      841 ttagttaatt tcataatgaa ccttacatga aatatttatt cgataggttt gatgctaaaa
      901 gttgaagttg cattgcgctt ttatcataaa tgcttagtcc tttttttatt tttttataaa
      961 taatctcata ctcatcttgt agatgcttag gtgggattaa tatctctata ttctgcaatt
     1021 cctgtgcatt aatattagcc atgccaacaa tacttttaca catattcatc aatgtgattt
     1081 tgccatggat ggaattaaga taccctgaaa tatagtagtt atttccgata gaattaggtc
     1141 taacgcggat taaatatcca gcaaatgcca taggcctgtc ttcttcatac acagctgttt
     1201 taccaacaag ttccttactg ttagtacgat tgaaaagaag atcgccttct ttaaccaaat
     1261 atttatcctt ttcttttaca ctgagatcaa gatattttaa atctgtaaaa tcccacctac
     1321 cttgatatgt tatattaccc atccgtaata taggatattc accagaatca atagaggctt
     1381 ttgctgaagt tccataattt actgaatcaa ccaagtcgcg aatcgttcct ttcgggaaac
     1441 gatgtatgtt gtttgcagga gttccaaaca tctctagaaa cttagcccgc aaaaaatcat
     1501 cagccagttt gatggcttgt tcgcgctttt ggcgtatagc atcagcttta tcaagaatag
     1561 cagcaatgcg tttttgttct tttaatgaag gaagcctaat tttaatatct tgtatctttt
     1621 ttacagacaa
//
LOCUS       DAJOTB010000324         1610 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJOTB010000324 DAJOTB010000000
VERSION     DAJOTB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.333"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..671)
                     /locus_tag="OHN28_004955"
     CDS             complement(<1..671)
                     /locus_tag="OHN28_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2300190.1"
                     /translation="MIKHTKPFLGIDYDLLAITSLVINGKTKEVKLHHKIIYSYLLSL
                     SGTYQKVTPSLKGIGERLGVGTDQTVRRHIKDLVSFGWLKIEERAGSTNAYIVLPYEQ
                     EAQTQQEATVKAVPDQLPPTEDNKQPSFDVLDDDWDAPLPWETEETPVSSSEKVANDN
                     EEDVLENFASLIIKQKNTRTGGASFIEFATSLAYKHRLKLPNGIEAYFSKNYPNVYDD
                     FDIPF"
     gene            complement(726..896)
                     /locus_tag="OHN28_004956"
     CDS             complement(726..896)
                     /locus_tag="OHN28_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300191.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(916..1104)
                     /locus_tag="OHN28_004957"
     CDS             complement(916..1104)
                     /locus_tag="OHN28_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300192.1"
                     /translation="MKWFTPEHVISAFKKGELTRHQIVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          452 a          317 c          267 g          574 t
ORIGIN      
        1 taaaatggaa tatcaaagtc atcatacaca tttgggtaat tcttagaaaa atatgcctca
       61 ataccatttg gtagctttaa cctgtgctta taggctaacg atgttgcaaa ttctataaat
      121 gatgctcctc ctgttctggt attcttctgc ttgataataa gagatgcaaa attctctaac
      181 acatcctcct cattatcatt agctaccttt tcacttgatg aaacaggtgt ttcttccgtc
      241 tcccacggca aaggcgcatc ccaatcatca tcaagaacat caaaagaagg ctgtttatta
      301 tcttccgtag ggggaagctg atcaggaaca gctttaacgg tagcttcttg ttgtgtctgg
      361 gcttcttgtt cataaggaag gacaatataa gcatttgtgc tacctgccct ttcctcaatc
      421 ttcaaccaac caaaagaaac aagatctttg atatgacggc gtaccgtctg atctgtacct
      481 actcctaacc gctcaccaat acctttcaaa gatggtgtta ccttttggta tgtgccagat
      541 aaacttaaca aataagaata aatgatctta tggtgtagtt ttacttcttt tgttttgccg
      601 ttaatcacca gggaagtgat tgctagtaag tcataatcta ttcctaaaaa aggttttgta
      661 tgtttaatca ttaaaaaagc tccttgatgt aatcacgctc tacggcgcta ctatggtgat
      721 aaaaatcact tgcttttatt tgagagtgtt tcagcctctc ttttcaacat atcaagaatg
      781 taatctgtga cagtgatacg tctacttctg gcttctttaa ataacacctg aaatacttca
      841 tcaggaatat gaaagcaagt gcgtggttgt cttttctttt ctgtagtttc gctcatagtt
      901 ttctccttct tttctttact ctgtttcaga ttctttttca ttttttctta gttcatcaat
      961 aatcttgagt gcttcattga agcaagctgc tcgctctgga taacctcgac tccttgccat
     1021 attacgattc attactattt gatggcgtgt cagctcacct tttttaaatg ccgagatgac
     1081 gtgctcaggt gtaaaccatt tcataaaaaa ctccttattc agttaaaaga aaaaacacct
     1141 tgcaagcctg acggcttcaa agtgcattga ttttgattat ttagagaaga atgtttttct
     1201 gtccctctaa tacctattat tacatataaa gaaacatttg tcaatacctt ttcataaaaa
     1261 atctgatctt tttcatgcac ttacattttt tcttagctat tcgtgctctt ctgtgtagtg
     1321 ctattcttca ttattgtcta aaacgctcta aaacgcgttc taacgagatt ttgataaaag
     1381 caatacattc ccttatgtaa tggctgttta gctcgccaga ggcttattta aggggcttat
     1441 tttgtatttg ttaggtatat gaaaaggtga tgctagatat aagatagaga gtcactttat
     1501 aaaacgctct gtaaagctct gtagtgagct tattatatgt aggcaatgcg tttccattcc
     1561 ctaccctgct taatgctgtt agagaagcat ttagacccct tatttttaat
//
LOCUS       DAJOTB010000325         1608 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJOTB010000325 DAJOTB010000000
VERSION     DAJOTB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.334"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..425)
                     /locus_tag="OHN28_004958"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="OHN28_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
     gene            complement(415..>1608)
                     /locus_tag="OHN28_004959"
     CDS             complement(415..>1608)
                     /locus_tag="OHN28_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HCQ2300193.1"
                     /translation="GIGTENNKADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIV
                     DLKDAKLVIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPS
                     GEVQFLGIFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFC
                     LNSIKESWPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAA
                     QQAELLWRHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWS
                     KIALRVMYEVAKEAGVVFDAISVADKELKVPSHLKVICEKAISQGKAVLLCTAPMPFS
                     DDELKTIGKYLHCSSNWNTVDYRLKNKVSAEVSAFKTFSFVNRPDENWTRTIYDMQGN
                     KQNGI"
BASE COUNT          448 a          381 c          300 g          479 t
ORIGIN      
        1 atacttttca ctgccaccgc ctggtggtga ataaggtgtg agcatcaatt tccagacgtc
       61 tcccgccagt gttatccagg tttcatacac ttttttatca atcactgaat cccagcaaaa
      121 atggatagac attggtgggt ttttggcttt attaaaaact gacgacatcc ctgatacttt
      181 ctcgctccat tgtccaacac ttcctggact ggcattagta ctcgccagag ttctgaactg
      241 ataatagatg ccatcggtat ctaataactc cacataggta acttctgctg gtagggaatt
      301 agggtaaaaa aattgaaata ccattcatca tagggtgcaa gcagtgatat tttttctttc
      361 gctgctgcgt taaagttaag aaccatagac gtaataacaa acagtaattt tatattaaat
      421 tccattttgt ttattccctt gcatatcgta tatagttctg gtccaatttt catccggccg
      481 attaacaaaa ctaaacgtct taaacgctga tacttcagca gacaccttat tttttaatcg
      541 ataatcgacg gtattccagt tggaagagca gtgtaaatat ttcccaatgg ttttaagctc
      601 atcatcagaa aaaggcatcg gcgctgtaca caagagaacg gctttaccct gagaaattgc
      661 tttttcacat attaccttta aatgactggg aacttttaat tccttatcag caacagaaat
      721 ggcatcaaat actacccctg cctctttagc gacttcatac attactctta acgcaatctt
      781 tgaccagtca ttggtgatcg ttctgcgaaa tgtcaccgct gcagcgaccc gttttctcac
      841 attacctaag tggtcaggcg acgctatttc atcaatattg gattcaattt ttaggacacc
      901 agaaggcaga atcggagcca gcgttgaatg gcgccacaat aactctgcct gctgtgctgc
      961 tttttggtaa gcgcttgtcg cttccaccgg cacatttccc agtaccgttt caatcgccgg
     1021 gcgacttaaa aacaggtact catcttccag tgggtcatag ccgccaccaa tatcagcatg
     1081 tgatccaggg aaacttaatt ctggccagct ttcctttatg ctgttgaggc aaaagttata
     1141 gcgacattcg ttcattgctg tgagctggaa tacatgtttg gccacacctg gcggtaactt
     1201 cacttttacc ggaaagttat taccatcatg agggtcaaaa ccatccatca gccctccgat
     1261 ggctgcaact gtgtcaaaaa tacctaaaaa ttgcacttcc ccggaaggtt tacctaaata
     1321 ttcaactgac tgaaatgccg tcgcaatggt tttaatcagt acgggatcac gttcaaatac
     1381 gcgattagta aagtgccgtg cggctgccgc cccacgacta aagccaaata cgtcaaactg
     1441 tagacgctta attactagtt tagcatcctt taaatcaaca ataatctttg ccagtgtctc
     1501 acgaagcagt gatatcgcac ggtcagtttt ggcaattacc ccagtatcat tatttcctag
     1561 ccccattcct accagagagt ctgctttatt attttcagtg ccaatacc
//
LOCUS       DAJOTB010000326         1603 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJOTB010000326 DAJOTB010000000
VERSION     DAJOTB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.335"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..913
                     /locus_tag="OHN28_004960"
     CDS             <1..913
                     /locus_tag="OHN28_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCQ2300194.1"
                     /translation="LIVLSSIYRELYSKGVKITILTNSKGGAFLSNNKNIFEFCIKES
                     TGFLKMLTLCKHLRDLQFDIVLDPFETMPSFKHSLILSSLKDSYILGFDQWYKRYYSF
                     YHPHDECLKEHMSTRATEILKHIYGEGEFSTDYDLHLPVDVEDKIKEFIGDTRIVIIN
                     PLGAKKICRLTFEQIKVIYQEMKLHFENYRVIFTGLPQDLLTIPIPEIETLPFNEFIY
                     TVALTKYSDFVISVDTALVHIAAAYHKPTLAFYPNSRTPEYPSHLIWSPNNHKSIQIV
                     SPTFTVKDIDTETLTDSVKRLSCIDKK"
     gene            complement(939..>1603)
                     /gene="waaL"
                     /locus_tag="OHN28_004961"
                     /gene_synonym="rfaL"
     CDS             complement(939..>1603)
                     /gene="waaL"
                     /locus_tag="OHN28_004961"
                     /gene_synonym="rfaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HCQ2300195.1"
                     /translation="SCAVLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIV
                     LLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFIKSP
                     FSFRSAESRAENMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIISILFLYF
                     SLFYTAYKRRALGLLILTLGIVGIGLSDVIIWARSIPIIVISAIVLLLVINNRNNTIN
                     QE"
BASE COUNT          541 a          274 c          270 g          518 t
ORIGIN      
        1 tctaattgta ttaagttcga tttatcgtga actttatagt aaaggggtta aaataaccat
       61 tcttacaaat agtaagggtg gggcgtttct ctctaacaat aaaaatatat ttgagttctg
      121 tattaaagaa tcaaccggtt tccttaaaat gcttactcta tgtaagcatc tgcgggattt
      181 gcagttcgat attgtattag acccctttga aaccatgccc tcatttaagc atagtttaat
      241 attatcaagt ttaaaagatt cttacattct gggttttgat caatggtata aacgttacta
      301 ttcattttac catccccacg atgaatgttt aaaagaacat atgagtacac gagcaacaga
      361 aatactgaag catatttatg gtgagggtga attctccacc gactacgact tgcatttacc
      421 agtagatgtt gaagataaaa taaaagaatt tattggagat acaagaatag tcattattaa
      481 cccattaggt gcaaaaaaaa tatgccgact tacgtttgag caaataaaag ttatatatca
      541 agaaatgaaa ctacactttg aaaattatcg ggtgatattt accgggttac cgcaagattt
      601 attgacaata ccaatacctg aaatcgaaac actaccattt aatgagttta tttataccgt
      661 tgcgttgacc aagtatagtg attttgtcat ttctgttgat acagctcttg ttcatatcgc
      721 tgcggcttat cataaaccaa cgcttgcatt ttatcctaat tcacgtactc cggaatatcc
      781 ctcacattta atttggtccc caaataatca taaatcaata caaatagtat ccccaacatt
      841 tacggtgaaa gatatcgata ctgaaacttt aactgattca gtaaagagat taagttgtat
      901 agataagaag tgagttttaa ctcacttctt aaacttgttt attcttgatt aatagtattg
      961 ttacgattat taataacgag taagaggact atagcggata taacgataat tggaatgctg
     1021 cgtgcccata tgatcacatc actgagtcca atccccacaa tgccaagcgt taatatcaac
     1081 aaacccaaag ctcgtctttt atatgctgta taaaacagtg aaaaatagag gaaaaggatg
     1141 gaaataattc ccatcagacc tttcagtgac cctgcttcaa ttatctcatt atgtagatgt
     1201 acgttagaaa actccaatgc ccctcttagc ctgttgtgtt ctgcaactaa caaattcata
     1261 ttttcagcgc gtgactctgc tgatctaaat gaaaaaggtg actttatgaa tatatttaaa
     1321 ccaatttcgt acattgccag tcttgcacct agggaagtca cgctattagc attggtataa
     1381 ctgtttaaat catttaatgc ttcattatag cgattctgta ttggtttatt aaatataatt
     1441 actatgctag ctaatatagc aattagtaga acaatagagg aagtgaattt cttgggtgat
     1501 ttattataat aagctattaa tgcagcaaca caaattatag ggaacaggag tagggttgct
     1561 ctggtttgtg ttagcgccag aacataaagt accgcgcaac tat
//
LOCUS       DAJOTB010000327         1590 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJOTB010000327 DAJOTB010000000
VERSION     DAJOTB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.336"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            340..1551
                     /gene="bluF"
                     /locus_tag="OHN28_004962"
     CDS             340..1551
                     /gene="bluF"
                     /locus_tag="OHN28_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCQ2300196.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          467 a          325 c          325 g          473 t
ORIGIN      
        1 tctgaagtca ctgaattttg atgcatgcta cgcctctgtt aaaaatgtta atcaatgtta
       61 agtgaggtaa caatagcaaa cttgtacgaa atatgtgtac agaatgataa attaattttt
      121 cgcaattagt gctaattcat ataaaacaat gcattacgat aaaacatatt gcatcattta
      181 aatgacaacc tatacaggtt ttttcaccaa accgtcttca accttttcca ctttttatca
      241 atgtaaagaa acggttatgt tttggtatta aaagaaagac acaagaagat atgtctgtta
      301 ccgtcttact ctcgcctcgc ccattaccct ggattgttaa tgcttaccac ccttatttat
      361 cgtagccata tacgtgacgc cgaacctgtc aaaaaaatcg aagaaatggt ttcgatagca
      421 aatcgcagga acatgcggtc tgacgtaaca gggatcttac tgtttaatgg ttctcatttt
      481 ttccagcttc tggaaggtcc ggaagaacag gttcaaatga tatatcgggc catatgccag
      541 gatccacggc actataatat tgttgaactg ttgtgcgatt acgcgcctgc tcgccgtttt
      601 ggcaaagcag gaatggaatt atttgattta cgcctgcacg agcgagatga cgttttacag
      661 gccgtattcg acaaaggcac atcaaaattt cagctaactt atgatgacag agcgctacaa
      721 ttttttcgta cttttgtcct tgcaactgaa caatcaacct atttcgagat tcctgctgaa
      781 gactcctggc tttttatcgc tgacggatct gataaagaac ttgattcttg taccctttca
      841 tcaaatataa acgaccactt tgcctttcat cctattgtcg accctttatc gcggcggata
      901 atcgcttttg aagccattgt gcaaaaaaat gaagacagcc cgtcagctat agcagttggg
      961 cagcgtaaag aaggggaaat ctacacagcg gatctcaaaa gtaaggcgct tgcattcgcg
     1021 atggcacacg cacttaagct cggtgataaa atgatttcaa tcaatctatt acctatgacc
     1081 ctggttaacg agcctgacgc agtctctttt ttacttgatg aaataaaggc caatgctctg
     1141 gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag tcatatctcg gtttgatgag
     1201 tttgccgaag cggttaaatc gctaaaggct gccggtatca gtgtagcaat tgatcatttt
     1261 ggcgcaggtt ttgctggttt gttactcctg tcacgcttcc agcctgacag aattaaaatc
     1321 agtcaggaat tgattaccaa cgttcataaa agcggaccac ggcaggcaat tattcaggcg
     1381 atcatgaaat gctgtacatc acttgaaatt caagtctctg ctatgggcgt ggcaacacca
     1441 gaagagtgga tgtggcttga atctgcagga attgagatgt ttcagggaga tctgttttcc
     1501 aaagctaaat tgaatggtat cccttcagtt gcgtggccgg agaaaaaata attttcagca
     1561 cattatttcg catgatttct ggaaatcatc
//
LOCUS       DAJOTB010000328         1555 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJOTB010000328 DAJOTB010000000
VERSION     DAJOTB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.337"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(97..957)
                     /locus_tag="OHN28_004963"
     CDS             complement(97..957)
                     /locus_tag="OHN28_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCQ2300197.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1140..>1555)
                     /locus_tag="OHN28_004964"
     CDS             complement(1140..>1555)
                     /locus_tag="OHN28_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2300198.1"
                     /translation="DLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRF
                     IDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNV
                     VLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          404 a          399 c          335 g          417 t
ORIGIN      
        1 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
       61 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
      181 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
      241 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
      301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
      361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
      421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
      481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
      541 cgttgtcaga agtaagttgg cagcagtgtt atcactcatg gttatggcag cactgcataa
      601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
      661 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
      721 taataccgca ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
      781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
      841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
      901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacga aaatgttgaa tactcatact
      961 cttccttttt caatattatt gaagcattta ccagggttat tgtctcatga gcggatacat
     1021 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1081 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1141 cacgaggccc tttcgtcttc aagaatttta taaaccgtgg agcgggcaat actgagctga
     1201 tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg tcagcacgac gttcctgtcc
     1261 acggtacgcc tgcggccaaa tttgattcct ttcagctttg cttcctgtcg gccctcattc
     1321 gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag ccgacaggat ggtgaccacc
     1381 atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa tgaaccggac tgccacgccc
     1441 tgagcgtcaa attcctttat cagttggatc atatcggcag tgtcgcggcc aagacggtcg
     1501 agcttcttaa ccagaatgac atcaccttcc tccaccttca tcctcagcaa atcca
//
LOCUS       DAJOTB010000329         1549 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJOTB010000329 DAJOTB010000000
VERSION     DAJOTB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.338"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(238..663)
                     /locus_tag="OHN28_004965"
     CDS             complement(238..663)
                     /locus_tag="OHN28_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HCQ2300199.1"
                     /translation="MTSLTPFRNVCPVTYSRFLSENFMEYQVREFINEKYTKAVNILK
                     DNLKENYHVFYGVRLSEILFPASEYGTDAFFKEFELINSVILPLVIFDLTQRKPMMII
                     SFDKILDASLLEGTNIVVLECITLADLLTNDNIDFLYKS"
     gene            complement(734..1177)
                     /locus_tag="OHN28_004966"
     CDS             complement(734..1177)
                     /locus_tag="OHN28_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300200.1"
                     /translation="MAKIYQFPQGDERGKFRKEIARERKKRFAVKTGSIFVKWLGWGW
                     FYLRLLAASVLHFVSVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPV
                     ITATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSGEISQ"
     gene            complement(1181..>1549)
                     /locus_tag="OHN28_004967"
     CDS             complement(1181..>1549)
                     /locus_tag="OHN28_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2300201.1"
                     /translation="EGLLRVMFSSKTINYITAIDSRKYQVTELPLKSALSLQGRYSWP
                     LYQLIKFSHLKSFTIGVDELKKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKC
                     VNAERQGRRVSKVRFEIEMR"
BASE COUNT          500 a          342 c          260 g          447 t
ORIGIN      
        1 taaacctgtt aattacgttt ccaacataaa attagtcttt attccaaaag acgcattaaa
       61 aacaaatagc gagacattcc gcaactggtt tcattctgca attagctgcg gaaaaataat
      121 atcactccct gattatctga aaaaaccagc acattatcat acacttcatt actcacatga
      181 agtagactca ataacacttg gcgacccaag cagaaaattt aaactgaaag gagattctta
      241 gcttttatat aaaaaatcaa tgttatcatt tgtcagaaga tcagcgaggg taatacattc
      301 aagaaccaca atatttgttc cttccaacaa actggcatca agtatcttat cgaagctgat
      361 aatcatcatt ggcttacgtt gtgttaaatc aaagattacc aatggaagta ttacactgtt
      421 aattaattca aactctttaa agaaagcatc agtaccatac tcactggcag gaaaaagaat
      481 ctcacttaat ctcacaccat aaaaaacatg gtagttttct ttaaggttat cctttaaaat
      541 attaacagcc ttagtatatt tttcgttaat aaactctctc acctgatatt ccataaaatt
      601 ctccgacaag aaccgactgt aggtcaccgg gcaaacgttg cggaatggcg tcagagacgt
      661 cattttgcgg cgtttgccct atcctgcatc gcagtgaaaa agcaggaact ggcataacgg
      721 ctgagtagca tcattactgg cttatctcgc cactgttctc ggtgctttca tccgcaggtt
      781 cgggaacaag taacttgtgc cagggcatag ttttgttgag cagctccatc agaggctcag
      841 ccagcagtcc caaaatccag gttgccgtaa tcaccggtat ggtcagattt tgtggcgtcc
      901 ataactggtg atcgagatga taccaggtga tgacacacaa aatccccccc agccagaaca
      961 cagcccattt aaacgcacca agaatggcta acacaaaaac agagacaaaa tgtaaaacac
     1021 tggcggccag aagacgaaga taaaaccatc cccagccaag ccacttaaca aaaatgctcc
     1081 ctgttttcac cgcaaaacgt ttcttacgtt ctctggcgat ttctttcctg aatttaccgc
     1141 gttcgtcccc ctgggggaat tgatagattt tagccatgag ttacctcatt tcgatctcaa
     1201 aacggacttt gctgactctt ctaccctgcc tttcagcatt aacacattta atgtttttaa
     1261 catctgtcct ttctgttatt tcatcaatag ctatattgat tccatctctt ttaaagaaag
     1321 aaaacttttg attttgaata taacctattt ctttcttaag ttcatctaca cctatagtga
     1381 aagatttaag atggctaaat tttatcagtt gatataaagg ccatgagtat cttccttgaa
     1441 gagacaaagc agattttaga ggaagctcgg taacctgata tttacgggaa tctatcgctg
     1501 taatataatt tatcgtctta gacgaaaaca taacacgaag caatccctc
//
LOCUS       DAJOTB010000330         1545 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJOTB010000330 DAJOTB010000000
VERSION     DAJOTB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.339"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(96..1216)
                     /locus_tag="OHN28_004968"
     CDS             complement(join(96..958,958..1216))
                     /locus_tag="OHN28_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HCQ2300202.1"
                     /translation="MKKRFSDEQIISILREAEAGVPAGELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     AAGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
BASE COUNT          408 a          415 c          369 g          353 t
ORIGIN      
        1 tagataggta gcattcctca ttaataatga ggacaccgag ggtagaatct taactccgtg
       61 ttaatatgac ctgcccccac gattagatac aacactcagt tagtaacgtc ggaatcttca
      121 ttctcagaat gaccctttct ccagcccgct gcaaattcag acggtgtctg ataattcagc
      181 gtggagtgcg ggcggcattc gttataatcc tgccgccagt cattaataat tttcctggca
      241 tgaacgatat cgctgaacca gtgctcattc aaacattcat cgcgaaatcg tccgttaaag
      301 ctctcaataa atccgttctg cgttggcttg cccggctgga ttaagcgcaa ctcaacacca
      361 tgctcaaagg cccattgatc cagtgcacgg caagtgaact ccggcccctg gtcagttctt
      421 atcgtcgccg gatagcctcg aaacagtgca atgctgtcca gaatacgcgt gacctgaacg
      481 cctgaaatcc caaaggcaac agtgaccgtc aggcattcct ttgtgaaatc atcgacgcag
      541 gtaagacact tgatcctgcg accggtggaa agtgcgtcca tgacgaaatc catcgaccag
      601 gtcagattgg gcgccgccgg acggagcagc ggcagacgtt ctgttgccag ccctttacga
      661 cgtcttctgc gttttacgcc cagaccactg aggtgataaa gccggtacac gcgcttatga
      721 ttaacatgaa gcccttcacg gcgcagcaac tgccaaatac gacggtagcc aaaacgcctg
      781 cgctccagtg ccagctcagt gatgcgccct gataaatgcg catcagcagc cggacggtga
      841 gcctcatagc ggcaggtcga cagggataaa cctgtaagcc tgcaggcacg acgttgcgac
      901 agaccggccg catcacacat caacatcacg gcttcccgct tctggtctgt cgtcagtact
      961 ttcgcccaag agccacctga agcgcctctt tatccagcat ggcttcggca agcagcttct
     1021 tgagtctggt gttctcttcc tcaagcgact tcaggcgctt aacttcaggc acctccatac
     1081 cgccatactt cttacgccag gtgtaaaacg tggcatcgga aatggcatgc ttgcggcaga
     1141 gttcaccggc gggtacccca gcttcggctt cgcggagaat actgatgatc tgttcgtcgg
     1201 aaaaacgctt cttcatgggg atgtcctcat gtggcttatg aagacattac taacatcggg
     1261 gtgtactaat caacggggag caggtcaaat agaataaaaa acaatcatca gaactgagta
     1321 gggaaagcga atcatgatgt atgaagtttc acacacaatg tgaatcaaaa acgctcgtag
     1381 ataatccgat agtgctgcct gatacgctga aagaaattgt tcgcaggaag atctgattat
     1441 gctgattcgt cgaccacagg cccttatata cataagcagc gcccgccagg tcagaaacaa
     1501 tgtgaacact atccgtgaga aattgtgaca aaattcccga tgatc
//
LOCUS       DAJOTB010000331         1534 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJOTB010000331 DAJOTB010000000
VERSION     DAJOTB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.340"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..147
                     /locus_tag="OHN28_004969"
     CDS             <1..147
                     /locus_tag="OHN28_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCQ2300203.1"
                     /translation="LAGMERWQWQDMPFWQVIAEAGMAAREASHAVREMERWMVPNKL
                     REAA"
     gene            422..583
                     /locus_tag="OHN28_004970"
     CDS             422..583
                     /locus_tag="OHN28_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2300204.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTA
                     SLAYESSGK"
     gene            complement(821..1434)
                     /locus_tag="OHN28_004971"
                     /pseudo
     CDS             complement(821..1434)
                     /locus_tag="OHN28_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          323 a          408 c          448 g          355 t
ORIGIN      
        1 ctggccggaa tggaacgctg gcagtggcag gatatgccat tctggcaggt tatcgcagag
       61 gctggtatgg cggcaaggga ggccagtcat gctgtccgtg agatggagcg gtggatggtg
      121 ccgaacaaac tccgggaggc ggcgtgatgc gcaacaatca cacggatttc ccgtgaacgg
      181 tctgaatgag cggattcttt tcaggaaaag tgagtgtggt cagcgtgcag ggatatgagc
      241 tatgatgtgc ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttaccgt
      301 gttgtgtggc agaaggcaga aagccccgta gttaattttt cattaaccca cgaggccccc
      361 tgtatgtctg atcaacaaca gtatggcctc ttaccgtgct taatgcaagg aggagtaaac
      421 catgaaacta ccgggaaacg cccttatctg gtgcgtatta atcgtgtgct gcacgttgtt
      481 aatattcaca ctcctgaccc ggaatcgtct gtgcgaagtc cggctgaagg acggatacag
      541 ggaggttacg gcaagtttgg cttacgaatc cagcggtaag tagcaacctg gaggcgggcg
      601 caggcccgcc ttttcagggc tgatgctggt ctgactgcac tgaagcgcct ttataaaggg
      661 gctgctggtt cgccggtggc ccctttctcc ttgctggcat tgtgcgggca tgaacaacca
      721 gactgccagg aaggccgttt ccctccggga acggggatga acgtgcgcct gcggcgcccg
      781 ttcttttccc ccgccttctc tgtttatgac ctgtcagaaa tcacatcacc ttccctttga
      841 ttattctctt tttcctcctg ttctgactct gattactgct gccctgatta ttgtctgttt
      901 tgttgttctc ctgcttatgc cttttccggg cgtccccctg gcggggcggg ctgtcgtgaa
      961 cggagccagc gaagctgtct ccgccctggc gggcctccat ccctgacgcg aaaccccggg
     1021 cttgcagcaa ccgttccggt cgcgccgcct ctgtcgcgcc gtggcctgcg ctcccggcat
     1081 cccggcaaaa ggccgccggg atgtaagggg caacgccgtt tcgcaaaccg cgcttcacgt
     1141 cgttgcggcg ggtcccgaaa ctcaaaagat gatgggggct gaaaaccaga gagaggagaa
     1201 agcaggatga gtacccgaaa tatccacgtt aacaccgcgt cgtacacgct tcttgttgcc
     1261 gggaagaaaa aaaacaccgg ggaagagtgg gacgtcctgg aattcagcag cctgactgag
     1321 ctgaaaaaat atcgcaaaag ccacccggaa aagatggcct tcagttatag ctacgcgctc
     1381 agtcagggtg tggataagca gttccgccat atcaacattg cggaagccga tcacttcaaa
     1441 cagttcctgc gtcagattaa gcgtgccggc ctcgatatcc gggcgatctg ctgaccggat
     1501 gtgatgaata cccctcctga ttgttgcaaa cttg
//
LOCUS       DAJOTB010000332         1528 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJOTB010000332 DAJOTB010000000
VERSION     DAJOTB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.341"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            98..316
                     /locus_tag="OHN28_004972"
     CDS             98..316
                     /locus_tag="OHN28_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCQ2300205.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
     gene            complement(501..950)
                     /locus_tag="OHN28_004973"
     CDS             complement(501..950)
                     /locus_tag="OHN28_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300206.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKFTTYDFRKNRSRYSF"
BASE COUNT          459 a          273 c          327 g          469 t
ORIGIN      
        1 aaaacaaaac gacatgcaaa aatccgcttt ttttaaatga tttttcggcg taattttcgc
       61 acatacatta atgagtaata tatgtaaggt ggtggcaatg gcgatgaata cggtttttct
      121 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
      181 gcgctctgca attttacgcg aagcggtaga gcaatatctg gagcgtcagc aatttccggt
      241 gcgtaaggca aaaagtgaca ggcaaagggg tgaggtagtt ggtgttgaag aacagggtaa
      301 gtggcatgag gaatgacaaa aagagtcgtg cttattaaca gcaatgtttt gtcattctgg
      361 aacataaaat caacatgcca gatgccttta ttctggcatc aatcagattg aatttgtgct
      421 cactaacagg aaaaattgat cacagggaat gcataaactt tatagatatc gctcctgata
      481 tcatccagga taataaacta tcaaaagctg tatctggacc ggtttttacg gaaatcataa
      541 gtggtaaatt tttcgttaca taatattctg ctatcgtatt cacttttatc gtcatgagat
      601 tttaggtaaa catccttatg caacccatga tttttcacaa ataaaaccag attaagattc
      661 ggctggtagt gattatgtgc ttcatcataa ccaaataaaa gctcttcccg gcggcagaga
      721 aaacaagctg tttttcctgg tgcgattttt aatttgatgt ggggacgtgg gaaattagtc
      781 caaagattgc catcgtcatc acactggtta atgttaaagc catagggtgc gaaagaagca
      841 actgacgcag ggtgtacact agttttaagc tggtttttgc ggcgaatcca ttcattagca
      901 gacagaaatt cccccgccat gatgacagcg gtatgaatat tatccttcat ccatttatcc
      961 gctaattctc tggaattaaa tgtgggtcgg ttagcggtaa atgtaagcgg aagcgagggt
     1021 agaggaaacg gtacaatatc acctatatca tcatgccata tatagtttgg ccattggttt
     1081 cctggttgta ggtgataacg cagggctttt gacccaccgg tataaaaatg ggcatctgct
     1141 ggtaatgtag aacgaacaat tcccttaccc ttaaaggcaa cttctcgcac aaaaaattct
     1201 aaaaataata ccggccaagg aagagtaagt ttgttaatca tagtcgaaat ccttcattat
     1261 tatgcttatt cagacagtat ctttgagctt ctggaacgtt tatccatttc ttttggccat
     1321 ttaaatagcc aattgtttct taaaaaaata aatggttgtg gtttgttctt tgcaccagac
     1381 acagttttat aagccatata attatggcaa taaagccata tttgggggat atagttcttt
     1441 tgtcttgcca tttcttgtgc actccacggt gcacgggttg tataaacgcg tgtctgtcgc
     1501 agtgcattta tatcattttc tctattaa
//
LOCUS       DAJOTB010000333         1527 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJOTB010000333 DAJOTB010000000
VERSION     DAJOTB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.342"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            329..769
                     /locus_tag="OHN28_004974"
     CDS             329..769
                     /locus_tag="OHN28_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300207.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDAL
                     RILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGV
                     IFEGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
     gene            complement(938..1189)
                     /locus_tag="OHN28_004975"
                     /pseudo
     CDS             complement(938..1189)
                     /locus_tag="OHN28_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
BASE COUNT          431 a          365 c          403 g          328 t
ORIGIN      
        1 aaaaaacgtg caggagctgt tgaacgtgaa aacctcgaaa tctggcaaaa atcacggctg
       61 acgaggcttg cagaggatta agcgaggctt gcagaggatt aagccagtat acactccgct
      121 atcgctacgt gactgggtca gggcttcgcc ccgacacccc aaagagtagc taggataaat
      181 atctaatgga atcaatgcgt tagacgccta atataaagat acagaaagga gaataaaaac
      241 attaacaaaa tctaagggtt acagttgaca tagttatcaa taatgataac actgttatca
      301 ttattgataa ctcaaatagg gttcaaagat gagtaaggat gtcgagagaa tcactcagat
      361 aaatgaccag ggtgagatta tcggcggctt cgttgccgtt atccgcccta aacaaaaatc
      421 agcatttcag aggcatttca ccatgaatca agacgcatta cggatcttgg ctaaggaatt
      481 aaccggggag caattcaagg tcttaatgct gatgcttgca gatctggatt acgaaaactt
      541 cattcagatc gcccaagcag atattgctga aactttaggg atgcagaaaa cgaatgttag
      601 tcgcgccgtt cgcgcgctac tggatgttgg ggtgatcttt gagggaccta aagttggcag
      661 atcaaaaaca taccggctga atgaacaatt tggttggaaa ggcaccgtaa ccaaccacaa
      721 aaaggcactc aaaaacggct ttagtgtcat tcagggcggc cggatttaaa gagccagcgg
      781 caggcggaca atcagggcta cgtgttaacg ttttgaccat gattgtctat cctgcattgc
      841 tcttttgccg catgtcagtt cgatgcgatg caggataggg caaacgccgc aaaatgacgt
      901 cattgacgtc attcgcaacc tttgcccgat aatctccaaa acgtctgagc tgccacggtt
      961 ccggcagcgc cttgagcgca accggaaacc gtctggggag aaccccagac ccccatcgct
     1021 gtaacatcga cggtctacga ccaccaacca gctgaatagc tgccagtgcc gtgcaaatgg
     1081 gctttgcagg gggctgacgc ggttcctggc gacctgaaag cccctggtta ggggtatcgg
     1141 cgcgcctgac ctcctgaaac cctctgtaag cgtttttcgc gccttccatg ccctgacggg
     1201 catttaaaac ccgtttcgtg cgcctgagag cttctgagag cgtttgaaga gctatctggc
     1261 gactcatccc cgaaagaaca acagcggaat caccgataac atgcgagagg tggcaccgct
     1321 cgccgcagcc ccagcgaccg aacgttagtg agcgagtggg cgaggaagcg gaagagaacc
     1381 ggaaaccaca ttgagcacgt acgcactgat gcggggtgtc ggggtgaagc cctgaccaga
     1441 tggcaaacgg aatatcgtcg cgggtgacgg tattacattt gcacatcctg tcccgatttc
     1501 tgcggagttt aacggtgaac ggacggc
//
LOCUS       DAJOTB010000334         1514 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJOTB010000334 DAJOTB010000000
VERSION     DAJOTB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.343"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            59..757
                     /gene="rfaY"
                     /locus_tag="OHN28_004976"
     CDS             59..757
                     /gene="rfaY"
                     /locus_tag="OHN28_004976"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCQ2300208.1"
                     /translation="MIQKSKIKDLVVFTDEENSKYLNVLNDFLSYNINIIKVFRSIDD
                     TKVMLIDTDYGKLILKVFSPKVKRNERFFKSLLKGDYYERLFVQTQKVRNEGLNTLND
                     FYLLAERKTLRFVHTYIMIIEYIDGIELCDMPDIDDSLKNKIQQSINALHKHGMVSGD
                     PHRGNFIIKNGEVRIIDLSGKRASAQRKAKDRIDLERHYGIKNEIKDLGYYLLVYRKK
                     MRNFMRRLKGKPAR"
     gene            827..>1514
                     /gene="waaZ"
                     /locus_tag="OHN28_004977"
     CDS             827..>1514
                     /gene="waaZ"
                     /locus_tag="OHN28_004977"
                     /EC_number="2.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HCQ2300209.1"
                     /translation="MKNIRYIDKKDVENLIENKTSDDVIIFLSGPTSQKTPLSVLRTK
                     DIIAVNGSVQYLLSHNIVPFIYVLTDVRFLHQRRDDFYKFSQRSRYTIVNVDVYEHAS
                     KEDKLYILQNCLVLRSFYRREKGGFIKKIKFNILSQIHKELLISVPLSKKGRLVGFCK
                     DISFGYCSCHTIAFAAIQIAYSLKYARIICSGLDLTGNCSRFYDENNNPMPSELSKDL
                     FKILPFFRFMR"
BASE COUNT          479 a          246 c          272 g          517 t
ORIGIN      
        1 caggagtctg ttatctttgc cgaaaatatt accctaaata acatttaact ggtttgttat
       61 gattcagaag agcaagatca aagacttggt tgtttttacc gatgaagaga attcaaagta
      121 cctcaatgta ttaaatgact tcttgtctta taatataaat atcatcaagg tttttcgttc
      181 tattgatgat acaaaagtta tgcttattga taccgattac ggtaaattga ttcttaaggt
      241 tttttctccg aaagttaagc gtaacgaacg tttctttaag tctctgttaa aaggtgatta
      301 ttacgaacgc ctttttgtgc aaactcaaaa agtacgaaat gaagggttaa atacactcaa
      361 tgatttttat ttattggctg aacggaaaac cttacgattt gtccatactt atatcatgat
      421 catcgagtat attgatggta tagagttatg tgatatgccc gatattgatg attcgctaaa
      481 aaataaaatt caacaatcaa ttaatgcctt gcataaacat ggcatggttt ctggcgaccc
      541 tcatcgtggt aacttcatta taaaaaatgg tgaggtccga attattgatc tctctggaaa
      601 acgtgcttca gcgcagcgta aagcgaaaga tcgcattgac ttagagcgtc attacggtat
      661 taaaaatgag attaaagatc taggctatta tcttttagta tatcgtaaaa aaatgcgcaa
      721 ttttatgcgg cgtttgaaag ggaaaccagc tcgctaaaaa aatcccacaa ttacatgtct
      781 tcaccaggac agttttaagc ggagttattt atttcacagg tttatcatga agaatattag
      841 atatattgac aaaaaagatg ttgaaaacct gattgaaaat aaaacatctg atgatgttat
      901 tatttttctt tcgggcccta catcgcaaaa aacacctttg tcagtattac gaacaaagga
      961 tattattgct gtcaatggtt ctgtgcaata tctgttaagt cataatatcg ttccttttat
     1021 atatgtactt acagatgtcc gctttttaca tcagcgtcgt gatgattttt ataaatttag
     1081 ccagagaagc cgttatacca tagtaaacgt tgacgtttat gaacatgctt ccaaagaaga
     1141 taaactttat atcctccaga actgcctcgt attacggtcc ttttatcgcc gggaaaaagg
     1201 tggttttata aagaagatta aatttaatat attgagtcag attcacaaag aactgctgat
     1261 ctctgtaccg ttgtctaaaa aaggtcgtct ggttggattt tgcaaggaca ttagttttgg
     1321 ttattgttca tgccatacta ttgcctttgc tgcaattcaa atagcatatt cacttaagta
     1381 tgcacggatt atttgttctg gtcttgattt aacgggtaac tgctctcgtt tctatgacga
     1441 aaataataat cccatgccct cggaattgag taaggattta ttcaaaatct tgccattttt
     1501 ccgttttatg cgtg
//
LOCUS       DAJOTB010000335         1496 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJOTB010000335 DAJOTB010000000
VERSION     DAJOTB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.344"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(99..1337)
                     /gene="alaC"
                     /locus_tag="OHN28_004978"
     CDS             complement(99..1337)
                     /gene="alaC"
                     /locus_tag="OHN28_004978"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCQ2300210.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          345 a          421 c          384 g          346 t
ORIGIN      
        1 cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg
       61 agccattagg ctcctgtttg catttctggc gaaggcgttt attccgcgtt ttcgtgaata
      121 tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca tcgctttaat cccacgaatc
      181 gcctgacgaa tacggtcgcg gttttcaatc agtgcaaagc gcacatgcgt gtcgccgtag
      241 tcgccaaagc caatccctgg agagacacag accttcgctt cgttaagcaa tttcttggca
      301 aattccagcg agcccatggc agcatacggt tccgggattt tcgcccagac atacatcgaa
      361 gccttaggca tttcgaccat ccagcccgct tcatgcagcc ctttcaccag cacatcgcgg
      421 cggcgtttgt actgttcggc aatatcgcgc acgcactgtt gatcgccctc cagcgccgca
      481 atagccgcca cctgcaacgg cgtaaaggtg ccgtaatcgt gatagctttt aatacgtgcc
      541 aacgcgctga ccagcgtttt gttaccgacc ataaagccga tacgccagcc cgccatgttg
      601 tagcttttcg acagcgtaaa gaactcgacc gccacgtcgc gtgcgcccgg cacctgcatg
      661 attgacggcg ctttccagcc atcgtagacg atatcggcat aggccaggtc gtggaccacc
      721 agcacatcgt agcgtttagc cagcgccaca accttctcaa agaattccag ttccacgcat
      781 tgcgcggttg ggtttgacgg gaagccgagg atcatcatct tcggtttcgg ataactttca
      841 cgaatggcgc gctccagttc attgaagaag tcgacacctt ccaccagcgg cactgagcgt
      901 acctgcgccc cggcaatcac cgcgccgtaa atatgaatgg ggtagcttgg attcggcacc
      961 agcactgtgt caccatgatc cagcgtcgcc agcatcaggt gcgccagccc ctctttcgaa
     1021 ccaatagtga cgatggcttc tgattccggg tcgatttcaa cgtcgtagcg atcctgatac
     1081 cagcgggaaa tggcgcgacg taaccgcgga atgccgcgtg aagtggagta accatgcgtg
     1141 tccgggcgct gggccaccgt acataatttt tcgacgatat gcggcggagt cgcaccgtcc
     1201 gggttaccca tactgaaatc gataatatct tcgccgcgcc gacgcgcagc cattttcagt
     1261 tcagcggtaa tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca
     1321 gggcgagtgt cagccataat ttcctcagat taacgttagc gcccggaccg tccgagcgac
     1381 gctgccacga tggtggctgt tttgaaaata gcctgattaa tttctgtctg tccagcgttt
     1441 tagaacattt ttatcaatgg ctttaaatga ggaactccac ttcaaaaaca ggaaat
//
LOCUS       DAJOTB010000336         1434 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJOTB010000336 DAJOTB010000000
VERSION     DAJOTB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.345"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            136..690
                     /locus_tag="OHN28_004979"
     CDS             136..690
                     /locus_tag="OHN28_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300211.1"
                     /translation="MVDLTKNGVEFYWSRNGSRGGGIGESIVTAIGVFKVNVKAEINI
                     TPSMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDS
                     AHEMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDM
                     YTRLVAADEDVQGLIWLSRIKFDD"
     gene            683..1105
                     /locus_tag="OHN28_004980"
     CDS             683..1105
                     /locus_tag="OHN28_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300212.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     ANVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQNICP"
BASE COUNT          475 a          197 c          293 g          469 t
ORIGIN      
        1 gcgaatgagg aagattgggt agatatagaa gttaacaaaa acaataagac agtaaatata
       61 atatggcgac tggcagttag tgatggaggg ataaaaggaa gtaatcctaa gttatcccca
      121 gttccttata acgacttggt tgacctgacc aaaaatggag ttgaatttta ttggtctcga
      181 aatggaagtc gcggcggggg gattggtgaa agtattgtta cagcgattgg tgtttttaaa
      241 gtaaatgtaa aggctgagat aaatattact ccatccatgc gaactttttc tttgatatca
      301 tcattagatc ctgactttca ggcatccgtt agtctatcag gatttgaaaa aatatattat
      361 aactacggtg atagttataa ggatattcaa gatgaattac aggcacttct tgacgctaat
      421 aacagatata aatgggatag tgctcatgaa atggggcata aagtactgga tgaatatgga
      481 gagggttcta gccctgatta ttcgtggact cataaaggaa catctactct tatgcaaaaa
      541 acaataccag ggaatgttat gcccgctcag ggagagattg atgtaatgaa atatggaaaa
      601 tatcgtcctg acatgtatac tcgattggtt gcagcagatg aagatgttca ggggttgatc
      661 tggttaagca ggataaaatt tgatgactag agtagttatt gctttattat ttcttgccgg
      721 atgtacttcg caaatgaagt attccaatgt cgtcaggatt aggaatgtat gttcctcaga
      781 tattaatgtg tttattcctg agtatgtttt tggagctaat gttattaagc aacatcatat
      841 ttacttacct gttaatggga ttgatgtttt gtcttatttt tatactgaaa acatcaatga
      901 tggtttccaa tatgaagaag cattcaccgt aaataaagat agctcgggta attttgacta
      961 tccgtttgtt atcttatcat caggacgtga aaaaaaattt gggaaaaacg atctaattga
     1021 attatccagg gacatttcaa caaaagacga taagaagcat ggggttgtaa agtacatgat
     1081 agatagtcag aatatttgcc catagtatga ttcatatcat aatcaactga aaaattgaca
     1141 ttattaatca tttgtaattt tttgatacta ccaagtgaaa ttttatgatt agattaatat
     1201 tctctttttt attatgcaca ctgtgtgctg gatgtttatc tcaaccattc tattcttgtt
     1261 ccattgtcat ggtaaataca tgcaaagtat ctattagcat ttcgacagtt aataattcaa
     1321 cacattatga tggtaaagta agagataatt tccatcttga tgtcggagaa cgaaagccaa
     1381 ttgtatactt tatttacaca ggtagagagt cagatgtttt taataaaaac cctg
//
LOCUS       DAJOTB010000337         1429 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJOTB010000337 DAJOTB010000000
VERSION     DAJOTB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.346"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..173)
                     /locus_tag="OHN28_004981"
     CDS             complement(<1..173)
                     /locus_tag="OHN28_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCQ2300213.1"
                     /translation="MVDSTRNRSAERLIDILVELQNYGVVSRYNLMKKYNITERTAYR
                     DLNMLSPFIEACG"
     gene            complement(597..1334)
                     /locus_tag="OHN28_004982"
     CDS             complement(597..1334)
                     /locus_tag="OHN28_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCQ2300214.1"
                     /translation="MKKVIAVSTLAMAGMFSAQTLADENKTGFYVTGKAGVSVMSLSE
                     QRFVDGEGAWADKYKGSDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESAGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGAGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
BASE COUNT          414 a          349 c          258 g          408 t
ORIGIN      
        1 tcgccacagg cttcaataaa gggagaaagc atattcagat cacgatatgc tgttcgctca
       61 gtgatgttgt attttttcat caaattatag cgactgacca cgccataatt ttgtaattca
      121 acaaggatat caatcaaacg ctccgctgag cgatttctcg ttgagtcaac cataaatcac
      181 tgcacctgta gtattactct tgtgtaagtg aattatataa atactcctga catcatttgt
      241 cagttccaca aaactacgcc ctccggcaac agatttttat ttgattatgt tcaaacttta
      301 aaccagatgt ccgggagcct ccttcgtaat acggtttatt gtttccggaa gggctgatat
      361 gacaaaaaac aacgtgtccc aacaacagtc ctttttcatt taacagactg acaaccatac
      421 tgcgtatcac ctgcacctct gtaaaaaatc aacgcgagta atatttttac tctatttata
      481 tttcattcat ctaaaattat tagttgccag agggagaata tcatcgggat atctgttgtt
      541 gacaatttgt catacctgca ctttactgtg ccccaaagcg caggtagaaa tggctgtcag
      601 aagttatacg ttgcgcctaa cataaactcg ttacttctga catcaacaga ggatttatat
      661 ttagcaccat cctcatcttt ataagtcaga gtggaatccc ctgcatccag ataccggtag
      721 ctgaggtcaa ggctgaaatc ctgcgtcaca tcgtatttaa caccggcacc caggctccat
      781 gccaggttgt tttcgtattt tgacgctgaa gctgaataaa cttcactgcc tgccgggctg
      841 ttatcggtat atatgtacga agttttatga tgaacccgtg cataacccag accagcagat
      901 atccatggcg tgaatgcact gctgtttctg aaatcataat acgcattcag catcagcgtg
      961 tttacactga gcttgttctg tgcatcatcg aactcggcac caccggcact ttcccaataa
     1021 ctcagacgat attttgattc agcgttaccg cggccataaa actccacttc agtacgcacc
     1081 ggcacattgt agtgctgata aaaatcgtat cccaccgcaa ggcctgcgcc aaaaaccgta
     1141 tcacttttgt cactgccttt gtatttatcc gcccatgccc cttcaccatc aacgaaacgt
     1201 tgctctgaaa gtgacataac agaaacaccg gctttaccgg tcacataaaa gccagttttg
     1261 ttctcatcag ccagagtctg ggccgaaaac atgcctgcca ttgcaagtgt tgaaacagca
     1321 ataacctttt tcatttcaat catctccgtt ggttcttacc ccacccgata aagaaggttg
     1381 ctcattttaa aaccatgcaa aagacaaggt aaaaagagaa aattcgatc
//
LOCUS       DAJOTB010000338         1425 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJOTB010000338 DAJOTB010000000
VERSION     DAJOTB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.347"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1072
                     /locus_tag="OHN28_004983"
     CDS             <1..1072
                     /locus_tag="OHN28_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2300215.1"
                     /translation="GGAVLDKLLDNCNNINLLLLVRAPTPQAGLERIKENMRKFNVCE
                     ERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGNNPFIWNVNVTGTL
                     AFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHLVEYTHSKATIEYL
                     MRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKFMCSLDDKIDVIPV
                     DYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALNCDPVGDRYTKVSY
                     DILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLLSIGMPKPPKFTDY
                     IKYCIETTKHLSIQQQMEVDFK"
     gene            1141..>1425
                     /locus_tag="OHN28_004984"
     CDS             1141..>1425
                     /locus_tag="OHN28_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HCQ2300216.1"
                     /translation="MSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYFMI
                     HHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLIPFSPH"
BASE COUNT          436 a          237 c          293 g          459 t
ORIGIN      
        1 tggtggcgcg gtcctggata agctgctgga taactgtaat aatataaatt tgctactttt
       61 agtacgagca cctactccac aagcgggact ggaaagaatt aaagaaaata tgcgtaaatt
      121 taatgtttgt gaggaaaggt tgcatgcatt aactaatgat aacatcttgc ctggggatct
      181 aaataatccg gaagcctttc tcatggatcc tcgtcttgat gaagtcactc atgttataaa
      241 ctgtgcggct atagcttctt ttggtaataa tccttttata tggaatgtga atgttacagg
      301 tacacttgct tttgcaagaa gaatggcaaa agtggcagga ctgaaacgct tccttcatgt
      361 tggtactgct atgtcttgta cacctcatac ggggtcgcta gttaaggaag agtctgcttc
      421 atcagaaaca ggtgaacatt tagtggagta tacgcattca aaagcaacaa tagaatatct
      481 gatgcgtaag cagtgtcctg atttaccttt gttggttgcc cgaccatcaa ttattgttgg
      541 ccacagtcgt ttagggtgct taccttcaac cagtattttc tgggtattca gaatggggtt
      601 aatgttgcaa aaatttatgt gctctctgga tgataaaata gatgttatcc ctgtagatta
      661 ttgtgctgat gcattgctaa tgttgcttga aagctcgtta attaatggtg agattgttca
      721 tatatcagca ggtaaagaaa gtagtgtgac gttctctgct attgacgaag ctgtagcccg
      781 tgctttgaac tgtgatcctg ttggagacag atatactaaa gtcagttatg acatactggc
      841 aatgagccgt catgatttta aaaatatttt tggtccctgt aacgaacgcc ttatgttaaa
      901 agccattcgt ttatatggag cgttcagtat gctcaatgtt tgtttcagta acgacaagct
      961 actgagtatc ggaatgccta aaccgccaaa gtttactgat tatattaaat actgtataga
     1021 aacgacaaaa cacctttcaa ttcaacaaca aatggaagtt gattttaaat aatatttatc
     1081 ataatcagaa ttattgttgg tgttgcactc ataatatata aaaacgccga ggatatgact
     1141 ttgtcaatta aaaatataaa gcgcattatt acggcctgga aaccatctac attcgaaacg
     1201 tacaaaaaaa cttttgaaaa atacggtggt agtgtaaaca tgcatccgga tgttgtttca
     1261 tactttatga tacatcatga ctggaagttt gatttttttc actatgaaaa agatggtgat
     1321 atcaaaggct catattttct gtgcaatgga aaacaaattg ggataatggc aagaaggtct
     1381 tatcctcttt catctgatga ggtattaatt cctttttccc cacat
//
LOCUS       DAJOTB010000339         1412 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJOTB010000339 DAJOTB010000000
VERSION     DAJOTB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.348"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            108..818
                     /locus_tag="OHN28_004985"
     CDS             108..818
                     /locus_tag="OHN28_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2300217.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPLIKATMQ"
     gene            860..>1412
                     /locus_tag="OHN28_004986"
     CDS             860..>1412
                     /locus_tag="OHN28_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCQ2300218.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGI"
BASE COUNT          392 a          337 c          293 g          390 t
ORIGIN      
        1 attaagtctg gaaacccggc gactcgccaa tgtattagca atatttaccg attgtctgat
       61 gtaacggttt ccccgcttat cctcttaaca catggagaat gcatcagatg caaactacgc
      121 gtacccccta ctctgtttcc tttatggcga cagtgttact tttatttctc tttgcttgcc
      181 atagcaccat tgccaacgca gctgtcgcac ttggtgcgac gcgggtaatt tatccggcaa
      241 accagaaaca ggttttattg cctgtcacga ataatgatcc tgccagtgtc tatctcattc
      301 agtcatggat agaaaatgcg ggtgaccaga aagataccca gtttgtcatt accccaccgc
      361 ttttttccat gcaaggtaaa aaagagaata cgcttcggat aatcaacgcc actaatcatc
      421 agttaccagg cgaccgtgaa agcctgttct gggttaatgt taaagctatc cctgctatgg
      481 aaaaagacca gaagaatgag aatacattac agctggcaat tattagccgc ataaaaatgt
      541 tttatcgccc cacgcatctg gcaatggcac ctgaagaagc gcctgcaatg cttcggtttc
      601 gtcgcagcgg tagcatactc actcttatta atccaacgcc ctacttcatt accgtaacaa
      661 atatgaaagc aggaaatagc aatctcccaa acaccatggt gccgccaaaa ggtgaagtat
      721 cggtggatat cccgcatgct gtaaccgggg atatcagttt tcagacaatc aatgattacg
      781 gtgcattaac tccgcttatt aaggcaacca tgcagtaagc cagtgaccta attaataata
      841 aactcaatcg ggattaaaaa tgacagcctt tcgtgccgca tttaaggcat atcgtatgca
      901 tcaggtgtta atactgcctc gctttgcacg gcttaccttt gcccttggct tagcaactgc
      961 ggtttttccc gttgacgcag agtattattt taatccacgg tttttaagca atgaccttgc
     1021 tgaatctgtc gatttatctg cctttactaa gggacgcgag gccccacctg gtacatatcg
     1081 ggtcgacatt tatttgaacg acgaatttat ggctagtcgg gatattactt ttattgcaga
     1141 tgataataac gcagatctca tcccatgcct gagtacagac cttcttgtca gtcttggcat
     1201 aaaaaaatca gcattattag acaacaagga acattcggca gataaacatg tgccggacaa
     1261 cagcgcatgc acaccacttc aggatcgttt ggctgacgca tcatctgagt ttgatgttgg
     1321 tcagcaacat ctgtcactct ccgttccgca aatatatgtc ggcagaatgg ctcgcggcta
     1381 tgtttctcct gatctgtggg aagaaggaat aa
//
LOCUS       DAJOTB010000340         1374 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJOTB010000340 DAJOTB010000000
VERSION     DAJOTB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.349"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..1209
                     /gene="gltS"
                     /locus_tag="OHN28_004987"
     CDS             4..1209
                     /gene="gltS"
                     /locus_tag="OHN28_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCQ2300219.1"
                     /translation="MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIV
                     AIVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTI
                     LIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELA
                     MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVI
                     ETISMLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVD
                     VLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYD
                     AVVISAGHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKI
                     FIEIGTYFT"
BASE COUNT          373 a          265 c          275 g          461 t
ORIGIN      
        1 aaaatgatcc tagatgccag ttatacatta ttagtcgcat gtatcgcgct actcatagga
       61 atgtttgtcg taaaatttac cccgttccta caaaaaaacc acataccaga agccgtcgtt
      121 ggtggcttta ttgttgcaat tgttctgtta attattgata aaacatcagg ttattcgttt
      181 acttttgatg cttcattgca aagtttatta atgctcacat tcttttcctc tatcgggcta
      241 agttctgact tttctcgact gattaaagga ggaaagccgt tagttctatt aactattgca
      301 gtaacgatcc taatcgccat tcaaaatact gtcggcatga gtatggctgt catgatgaat
      361 gaaagtccat ttattggctt aattgcaggt tcaattactc taacaggtgg tcatggtaat
      421 gccggagcat ggggccctat tctcgctgat aaatatggtg taacaggcgc cgttgaatta
      481 gcgatggctt gtgcaacact tggattagtg ttgggtggtt tagttggcgg ccctgttgcc
      541 cgtcatcttt tgaaaaaggt ctctattcct aaaacaaccg agcaagagcg cgacactatc
      601 gttgaagctt ttgagcaacc aagcgtcaaa agaaaaatca atgcaaataa cgttattgaa
      661 accatttcaa tgctgattat ctgtattgtt gttggtggct atatcagtgc attgtttaaa
      721 gatacttttc tgcaactgcc tacttttgtc tggtgtttat ttgtcggtat tattatccgt
      781 aatacactga ctcatgtatt taaacacgaa gtgtttgagc cgaccgtcga tgtattaggt
      841 agcgttgctt tatcgctttt cttggcaatg gcgttaatgt cattaaaatt tggtcaattg
      901 gcaagcatgg cagggccagt attaattatc attgctgtac aaactgttgt catggtgcta
      961 tttgcctgct ttgtcacctt caaaatgatg ggcaaagatt atgatgctgt cgtgatcagc
     1021 gcgggtcact gtggctttgg tatgggagca acaccaacag caattgcgaa tatgcaaaca
     1081 gtcacaaaag catttggacc atcacataaa gctttccttg tcgttcctat ggtcggtgcc
     1141 tttattgttg atatttcaaa cagtatctta attaaaatat ttattgaaat tggtacatac
     1201 tttacctaat agctacacac tactcggcgt acttctccta acaatttatt atagggcagt
     1261 atgccgagcc gctttcttac cccgtatatt cccctagtca atctatttag cttgcacgtt
     1321 tacgaattaa ttctcgtgac aactaaacca aaatttgaat tatgacgagt atat
//
LOCUS       DAJOTB010000341         1355 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJOTB010000341 DAJOTB010000000
VERSION     DAJOTB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.350"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..956
                     /gene="ldtC"
                     /locus_tag="OHN28_004988"
     CDS             <1..956
                     /gene="ldtC"
                     /locus_tag="OHN28_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCQ2300220.1"
                     /translation="KTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGENKFH
                     VVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPREGIV
                     INIAELRLYYYPPGKNSVTVYPIGIGQLGGDTQTPTMVTTVSDKRANPTWTPTANIRA
                     RYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCIRLR
                     DDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPITLN
                     SAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1039..1296)
                     /gene="bhsA"
                     /locus_tag="OHN28_004989"
     CDS             complement(1039..1296)
                     /gene="bhsA"
                     /locus_tag="OHN28_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCQ2300221.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          338 a          345 c          345 g          327 t
ORIGIN      
        1 tcaaaacgcg tttttctcgc tggctaacat tttttacgtt cgccgctgcc gtggcgctgg
       61 cgctaccggc aaaagccaac acctggccgc tgccgtcagc aggcagtcgt ctggttggtg
      121 aaaacaaatt tcatgttgtg gaaaatgacg gtggttctct ggaagccatc gccaaaaaat
      181 ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct tacgtaccgc
      241 gcgcgggtag cgtgttaacg atcccgttgc aaaccctact tccagacgcg ccgcgcgaag
      301 gcattgtgat caacattgcg gagctgcgtc tctattatta tccgccggga aaaaattcgg
      361 taaccgtata tcccatcggc attggtcaat taggtggtga cacgcagaca ccgacaatgg
      421 tgaccaccgt ttctgacaag cgtgcaaacc caacctggac gccaacggca aacattcgcg
      481 cgcggtataa ggcacaggga attgagttgc ctgcggtagt tccggctgga ccggataacc
      541 caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg cttcacggta
      601 cgaacgctga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg
      661 acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat atcatcaaca
      721 ccccgattaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc
      781 tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat agcgcaatgc
      841 aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg atggatgtcc
      901 gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact ctgtaaagct
      961 cggataaaaa aatccccgcg acacggcatg tcgcggggaa aaaaacaagc agtagcaggc
     1021 attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg tattcggacc
     1081 ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt gcgccagctg
     1141 ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga ctttttgttg
     1201 gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa
     1261 aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat gcagatgaat
     1321 gacgtcgcaa gcaattactt aacgatcgat aacta
//
LOCUS       DAJOTB010000342         1256 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJOTB010000342 DAJOTB010000000
VERSION     DAJOTB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.353"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1256
                     /locus_tag="OHN28_004990"
     rRNA            <1..1256
                     /locus_tag="OHN28_004990"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          292 c          395 g          256 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      721 gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca tggctgtcgt
      781 cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt
      841 tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg aggaaggtgg
      901 ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct acaatggcgc
      961 atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg
     1021 attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg gatcagaatg
     1081 ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt
     1141 gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg attcatgact
     1201 ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac ctcctt
//
LOCUS       DAJOTB010000343         1240 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJOTB010000343 DAJOTB010000000
VERSION     DAJOTB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.354"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(236..>1240)
                     /locus_tag="OHN28_004991"
     CDS             complement(236..>1240)
                     /locus_tag="OHN28_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCQ2300222.1"
                     /translation="DLAPLFSAHSTRNYLLSTLDFTDSVGVTSHKVAVSQLVESNESL
                     FNKETSRFSSEHNVTKREQGKEWLIEIPYYLREDVIRPSDVQGKRKPGTDFQETLTDI
                     YADYVAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAMKATVNASS
                     TDTTKIFKEFDQIATNIIDRAQSQASAVERIVVFCKPEAFSAIRFSAGMANAFQYVSP
                     LEEGNVVYQRRDLLPGVTAFTIPGTNIDVIKLVDPLHLAHMTADAVAVPKFAKGSNVY
                     QNIYGAASSTFELINAAPTEVYSYSYESSRGDAVNVVTENSQMVVNHGVGFSVQITVK
                     "
BASE COUNT          376 a          273 c          250 g          341 t
ORIGIN      
        1 actctcgttt aatctgaaca tacgggcttt tacgccttca tagggcacat ctaggagaga
       61 acatagatat ttcagattta acaagcctct agaatcatta gcatctaaat gaacgtgtac
      121 accatttttt gatttcaaaa tcacttccat attttaatca cctctcaaaa tttaaaaaaa
      181 aataaaaaaa ggcgaagcgt gaatacacgc cacgcctcta ttacacgtta aaatattatt
      241 taacagtgat ttgaacggag aagcctacac cgtggtttac aaccatctgg ctattctctg
      301 taacaacgtt aacagcatca ccacgagaag attcatagct atagctgtaa acctcagtag
      361 gagcggcatt aatcagttca aaggtgctgg aagcagcccc gtagatgttc tgatatacgt
      421 tagagccttt agcgaattta ggcacagcta cagcgtctgc ggtcatatgt gccagatgta
      481 gcggatctac cagtttaata acatcaatgt tagttcctgg aatagtgaac gctgtcactc
      541 ctggcagcag gtcacgacgc tgatacacaa cgttcccctc ttccagtggg cttacatact
      601 ggaatgcgtt tgccataccc gcagagaatc gaattgcaga gaaggcttcc ggcttacaga
      661 aaacaacaat acgctctacc gctgacgcct ggctttgtgc tctgtcgata atattcgtag
      721 caatctgatc aaattcttta aaaatcttcg tggtgtcagt gctggaagcg ttcacagtgg
      781 ctttcatagc tgatacgtta aacagcttgc cccactcaat taacacatca tcagttttag
      841 gcgtgtaagt tttgccagag aacaaagaag cagccaatac gctctctttt gtacgctgat
      901 acgctacgtg gtgtttagca acatagtcag cataaatatc agtgagtgtt tcctggaaat
      961 cagtgccagg cttacgtttg ccttgaacat cagaaggtct aattacatcc tcccgaagat
     1021 aataagggat ttcaatcagc cattctttac cttgttcacg cttagtcacg ttatgctcag
     1081 aagagaaacg agaggtttct ttgttgaaca gagattcgtt gctttcgaca agctgggaaa
     1141 cagctacttt atgagatgta acccctacag aatccgtaaa atccagagta gaaagcagat
     1201 agtttctagt gctatgcgca gagaacaaag gagctaaatc
//
LOCUS       DAJOTB010000344         1223 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJOTB010000344 DAJOTB010000000
VERSION     DAJOTB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.355"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..766
                     /gene="yeeO"
                     /locus_tag="OHN28_004992"
     CDS             <1..766
                     /gene="yeeO"
                     /locus_tag="OHN28_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCQ2300223.1"
                     /translation="LWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVESVL
                     FTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQIA
                     QAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPI
                     WSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFADW
                     AVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            867..942
                     /locus_tag="OHN28_004993"
     tRNA            867..942
                     /locus_tag="OHN28_004993"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:900..902,aa:Asn,seq:gtt)
BASE COUNT          281 a          245 c          326 g          371 t
ORIGIN      
        1 tttgtgggtg ctggcgattg gttttaatcc tgcgctaagg atttcgttaa agagctattt
       61 taaaccgctg aattttagca ttatctggga agtcatgggg attggtattc cagcgagtgt
      121 cgaatcagtg ttatttacca gtggtcggtt attaacccaa atgttcgttg ccgggatggg
      181 gaccagcgtt attgccggaa attttatcgc gttctcaatt gcggctctta tcaacttacc
      241 cggaagtgcg ctcggttctg cttctacgat cattacaggc cgaaggttgg gggtagggca
      301 gatagcgcaa gcagagattc agttgcggca tgtgttctgg ctgtccactc ttggattaac
      361 ggccatcgcc tggctaacgg ctccctttgc cggggttatg gcatcgtttt acacccagga
      421 tccacaggtt aaacacgtcg ttgtgattct gatttggcta aatgctttat ttatgcctat
      481 ttggtccgcc tcatgggtgc tacccgctgg atttaaaggt gctcgtgatg cccgttacgc
      541 catgtgggtt tcgatgttga gcatgtgggg ttgtcgggtt gtagtcggtt atgtgctggg
      601 aatcatgctt ggctggggtg tggttggtgt ctggatggga atgtttgccg actgggctgt
      661 gcgggccgtg ctgttttact ggcgaatggt tactggacgt tggctatgga aataccctcg
      721 atccgagccg caaaagtgtg aaaaaaagcc agttgtgtcg gaataaacga caaaatgcag
      781 attatttcag caaacgattt caaatttaaa aaacaggctt tgacattgtg ggtgggcatc
      841 gctaatattc gcctcgttct cacgattcct ctgtagttca gtcggtagaa cggcggactg
      901 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattttag ttttgagaca
      961 tctctatgag tctcaggaca gttaaaaaat aaaaattatt tttacccggt cactttgtaa
     1021 aaaagtggcc gggtttttga tttctgtgtt tcgtgcgctt aatcgagtct cacgcttcag
     1081 tgagcaaatt gcccctcccc aacagacatc acgattcaca aggtcaaaaa ttctgttcca
     1141 tgaagtaccg tacaaagata aagagaaaaa tgccttatgc gggatgtagc tgtcgcgtct
     1201 ttatcgggaa actatacgta att
//
LOCUS       DAJOTB010000345         1221 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJOTB010000345 DAJOTB010000000
VERSION     DAJOTB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.356"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            115..480
                     /locus_tag="OHN28_004994"
     CDS             115..480
                     /locus_tag="OHN28_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2300224.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
     gene            520..1215
                     /locus_tag="OHN28_004995"
     CDS             520..1215
                     /locus_tag="OHN28_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCQ2300225.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
BASE COUNT          294 a          286 c          334 g          307 t
ORIGIN      
        1 cgaaatcaaa gataaataac atcgttcaaa tcgatttttc ctgtattcac cgccgttgcg
       61 caaaatttat ctatttgttc aaaaaatgat tgagtcttga ctggctcatc caatgtggaa
      121 aaatgtgact tttatcacat aatagttcta agtctgaatt ttccgggtta tctcaaaatg
      181 gaatacggtt cgacaaagat ggaagagaga ctctctcgca gccctggcgg caaattggct
      241 ttgtgggctt tctatacatg gtgtggctat ttcgtctggg caatggcgcg atacatctgg
      301 gtgatgagcc ggatccccga tgcccccgtg tcgggatttg aaagtgattt aggatctacc
      361 gcggggaagt ggttaggagc attggtcgga tttctgttta tggccctggt tggtgccttg
      421 ttaggcggca ttgcatggta cacccgacct cgtcccgcac gttgcagacg ctatgaataa
      481 aaactgctaa ggttgcgact ctttggcaac ggagcgcgta tggagttaca acactggcag
      541 gcacagttcg ataactggtt gaaaaaccat catcaacatc aggacgcggc gcatgacttg
      601 tgtcattttc gccgtgtctg ggctacggca cagaaactgg cggcagatga cgatgtcgat
      661 atgctggtga ttttaaccgc ctgttatttt cacgatatcg tgaccctggc gaaaaatcat
      721 ccgcaacggc agcgttcttc aatcctggcg gcagaagaga cgcgtcgcct gctccgtgaa
      781 gagttcgcgc aatttccggc ggagaaaatt gaggccgttt gtcatgccat tgccgctcac
      841 agtttcagcg cgcaaatcgc ccccttaaca acggaagcta aaatcgtcca ggatgcggat
      901 cggctggaag ccctgggggc gattggcctg gcgcgcgtat ttgccgtttc aggggcattg
      961 ggcttggcgc tgtttgatgg cgaagatccg ttcgcacagc atcgcccgct tgatgataaa
     1021 cgctacgcgc tggatcattt ccagacaaag ttactgaaat taccgcaaac catgcaaacc
     1081 gcaagaggca agcagctggc acagcacaac gcgcaatttt tagtcgagtt tatggcgaag
     1141 ctcggtgccg aactggcggg ggagaatgaa ggtatcgatc acaaggtgat agatgcgttt
     1201 tcacccgctg gctgagcgcg t
//
LOCUS       DAJOTB010000346         1220 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJOTB010000346 DAJOTB010000000
VERSION     DAJOTB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.357"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..561
                     /locus_tag="OHN28_004996"
     CDS             <1..561
                     /locus_tag="OHN28_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300226.1"
                     /translation="NLLEQIKEEGKQSTSEVFIKMKIAYIVTIMENCLSEMIKSVVLS
                     HNRYVENAIRNINELKAKNISLSELINKESNANKYVQEYLSDILYHRIQLVVEIYKAV
                     LQPKQYPRFPLKNINELMKLRHDIVHRNGKTKTTDEKIHTFNTATLNDAFKVVEEFLN
                     NMMNLISDAVEHHENEQIARDLEDEF"
     gene            612..>1220
                     /locus_tag="OHN28_004997"
     CDS             612..>1220
                     /locus_tag="OHN28_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300227.1"
                     /translation="MATKKYELTKEYFFHGEFWHQLDDNKGRFSARIEYSPYHGLILD
                     YCISDSESPRTCEILYGVLNTGERCTLIGKFDFTQGNIHFGKGIIHTGRHGFPIMLFN
                     DFYAPDSKIEYCDLSLHGLQEFIHPHGFFTQLKHLEHPIFIAKGNHWTLQLVNHVSFS
                     VIGDDLLNIINCQNKAALENIIHQLKKTKELYPDAFFSIRKEL"
BASE COUNT          461 a          197 c          212 g          350 t
ORIGIN      
        1 aaccttttag aacaaataaa agaagaggga aaacaatcta caagtgaagt tttcataaaa
       61 atgaaaatcg catacattgt gacaataatg gaaaactgtc ttagcgaaat gattaaaagc
      121 gttgtattgt cacataacag atatgtagaa aatgccataa gaaacataaa tgaactgaaa
      181 gcaaagaata tctctctgtc tgagttgata aataaagagt ctaatgcaaa caaatatgta
      241 caagagtacc tttcagatat tctttatcat agaatccaac tggtagttga aatatataaa
      301 gcagtcctgc aacctaagca atatccacgt tttccactca aaaatatcaa tgaattgatg
      361 aaactgaggc atgacatagt tcatcgtaac gggaaaacga aaacaacgga tgaaaagata
      421 catacattca atacagctac gctcaatgat gcgtttaaag ttgtagagga gtttcttaat
      481 aacatgatga atttgatcag tgatgctgtt gagcatcatg agaatgagca gatagccaga
      541 gatcttgaag acgagttttg agtagtattt ttactattct gaaggattct cagaaacaaa
      601 cggagcagta catggcaaca aaaaaatacg aactaacaaa agaatacttt tttcatggtg
      661 aattttggca ccaacttgat gataacaaag gaagattttc tgcacgaatt gaatattcac
      721 cgtaccatgg tctgattcta gattattgta tttctgacag tgaaagcccc agaacatgtg
      781 aaatattata tggagtctta aatactggag aaagatgtac tctaattggg aaatttgatt
      841 tcacacaagg gaatattcat tttggtaaag ggattatcca tactggaagg catggatttc
      901 ccataatgct attcaatgat ttttatgccc ccgactcaaa gatagaatat tgtgatttat
      961 ccttgcacgg attgcaagaa tttatccacc ctcacggttt tttcactcaa ttgaaacatt
     1021 tagaacatcc aatatttatc gcaaaaggaa accattggac tcttcaactt gtcaaccatg
     1081 tatcttttag cgtaattggt gatgatctat taaatataat aaattgccaa aacaaagccg
     1141 cactggagaa cataattcat caactaaaaa agacaaaaga gttataccct gatgcttttt
     1201 tctccattag aaaagaactt
//
LOCUS       DAJOTB010000347         1180 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJOTB010000347 DAJOTB010000000
VERSION     DAJOTB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.358"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1180
                     /locus_tag="OHN28_004998"
     CDS             <1..>1180
                     /locus_tag="OHN28_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCQ2300228.1"
                     /translation="HDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTK
                     ARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMV
                     LRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVP
                     WYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPI
                     SCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIRTPKQLVN
                     IYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQK
                     QGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNLFTHGYVL
                     G"
BASE COUNT          348 a          296 c          241 g          295 t
ORIGIN      
        1 cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa cagcttaacg
       61 ttggcttgcc acgccttact tgactgtaaa actctcactc ttaccgaact tggccgtaac
      121 ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt gttaggtaat
      181 cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt tatctgttcg
      241 ggcaatacga tgcccattgt acttgttgac tggtctgata tccgtgagca aaaacggctt
      301 atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta tgagaaagcg
      361 ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc cgaccttgcg
      421 agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt taaagtgcca
      481 tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag aggaaaagta
      541 caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca tgatatgtca
      601 tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc aatctcatgc
      661 caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc gacacggact
      721 cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc atggattcta
      781 gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat ctattcgaag
      841 cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg actaggccta
      901 cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat cgccctgatg
      961 cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg ggacaagcac
     1021 ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt aggcatggaa
     1081 gttttgcggc attctggcta cacaataaca agggaagact cactcgtggc tgcaaccctg
     1141 cttactcaaa atctattcac acatggttac gttttgggga
//
LOCUS       DAJOTB010000348         1152 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJOTB010000348 DAJOTB010000000
VERSION     DAJOTB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.359"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            15..968
                     /gene="ompT"
                     /locus_tag="OHN28_004999"
     CDS             15..968
                     /gene="ompT"
                     /locus_tag="OHN28_004999"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HCQ2300229.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          357 a          249 c          249 g          297 t
ORIGIN      
        1 aatggagacc ttttatgcgg gcgaaacttc tgggaatagt cctgacaacc cctattgcga
       61 tcagctcttt tgcttctacc gagactttat cgtttactcc tgacaacata aatgcggaca
      121 ttagtcttgg aactctgagc ggaaaaacaa aagagcgtgt ttatctggcc gaagaaggag
      181 gccgaaaagt cagtcaactt gactggaaat tcaataacgc tgcaattatt aaaggggcaa
      241 ttaattggga tttgatgccc cagatatcta tcggggctgc tggctggaca acactcggca
      301 gccgaggtgg caatatggtc gatcaggact ggatggattc cagtaacccc ggaacctgga
      361 cggatgaaag tagacaccct gatacacaac tcaattatgc caacgaattt gatctgaata
      421 tcaaaggctg gctcctcaac gaacccaatt accgcctggg actcatggcc ggatatcagg
      481 aaagccgtta tagctttaca gccagaggtg gttcctatat ctacagttct gaggagggat
      541 tcagagatga tatcggctcc ttcccgaatg gagaaagagc aatcggctac aaacaacgtt
      601 ttaaaatgcc ctacattggc ttgactggaa gttatcgtta tgaagatttt gagctaggtg
      661 gcacatttaa atacagcggc tgggtggaag catctgataa cgatgagcac tatgacccag
      721 gaaaaagaat cacttatcgc agtaaagtca aagaccaaaa ttactattct gttgcagtca
      781 atgcaggtta ttacgtaaca cctaacgcaa aagtttatat tgaaggcgca tggaatcggg
      841 ttacgaataa aaaaggtaat acttcacttt atgatcacaa tgataacact tcagactaca
      901 gcaaaaatgg tgcaggcata gaaaactata acttcatcac tactgctggt cttaagtaca
      961 ccttttaaca acgttaaata gattttctcc ccggtggtat tgtatagccc cggggaattt
     1021 gtttaccgtt aaaagcaaac aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg
     1081 cgtgtaacct cttaccgcaa cacaagactc aatatattca aattcgttat gccgtggcat
     1141 catttaaccc ct
//
LOCUS       DAJOTB010000349         1148 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJOTB010000349 DAJOTB010000000
VERSION     DAJOTB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.360"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1056
                     /locus_tag="OHN28_005000"
     CDS             <1..1056
                     /locus_tag="OHN28_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2300230.1"
                     /translation="GKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEK
                     LTQKISATNTFHALATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLE
                     MLEILRRIEKRGSLEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFP
                     HLRADELPVFLRKLAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIP
                     ESRMKMRRKHVVPLSRQTTDILLQLKTFSGQYQLVFPGRCDINKPMSEASINMVLKRI
                     GYDGRATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMM
                     QWYADYIDSLSRQESQG"
BASE COUNT          314 a          294 c          265 g          275 t
ORIGIN      
        1 ggaaaagaga aaaaactggc cctcggcgtc tatcccgacg tctcccttgc tgaagccaga
       61 atcaaacgcg acgatgcccg aaaaattatc tccgaaggtg gtgacccggg cgaaaagaag
      121 cgaaaggaaa aactcactca gaaaatctct gccaccaata cgttccatgc cctcgctacg
      181 gaatggcacc agcataaatc tttgtcatgg tctgaaagtt acgccagaag cgtactggaa
      241 gcgctggata aagatatttt cccgtatctg ggcaaacgaa gcgttacgga tatcctcccg
      301 ctggaaatgc tggaaattct gcgccgcata gaaaaacgtg gctcgctgga gaaactgcgt
      361 aaggtacgcc aatactgtaa tcagattttt cgttatgcca tcgccaccgg acgagccact
      421 gtcaatccgg catctgaact gaccagtacg ctggcggcac caaaagctgc acatttcccc
      481 cacctgagag cagatgagct ccctgttttt ctccggaagc tcgctgaata tcatggcagt
      541 cctgttaccc gcatggcgac aaatctgctg cttctgacag gcctcagaac gattgaacta
      601 cggtccgctg aatggtcaga aattgatttt gataatgccc tgtggacaat ccctgaaagc
      661 cgcatgaaaa tgcgacgtaa acatgtcgta ccactgtcac gacagaccac tgacattctg
      721 ctgcagctca aaactttctc cggacaatac cagctggttt ttccgggacg ttgtgatatc
      781 aacaagccaa tgagcgaagc cagcatcaac atggtgctca aacgtatcgg ttacgatggc
      841 agggcaaccg gtcatggttt tcgtcacacc atgagtacca ttctgcacga acagggcttt
      901 aattctgcct ggattgaaat gcagttagct catgtggata aaaacgccat caggggtacc
      961 tataatcatg cccaatatct cgatggtcgc cgtgaaatga tgcaatggta cgcagattac
     1021 attgattcgc tttccaggca agagagtcag ggttaagaac acttgttctg atattttctc
     1081 tcaatccaca agttctcctc cggcctccag gtccggagga attttgtttt catgctttat
     1141 ttttagat
//
LOCUS       DAJOTB010000350         1143 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJOTB010000350 DAJOTB010000000
VERSION     DAJOTB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.361"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            606..857
                     /locus_tag="OHN28_005001"
     CDS             606..857
                     /locus_tag="OHN28_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCQ2300231.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            854..1141
                     /locus_tag="OHN28_005002"
     CDS             854..1141
                     /locus_tag="OHN28_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2300232.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          319 a          285 c          269 g          270 t
ORIGIN      
        1 agatatgcac ctcccaccgc aagcggcggg ccccgaccgg agccatttta gttacaacac
       61 tcaaatacaa ccgccaagaa aaccccggtc cagcgcagaa ccgaaaccac aaagcccctc
      121 cctcataact gaaaagcggc cccgccccgg cccaaagggc cggaacagag tcgcttttaa
      181 ttatgaatgt tgtaactacg ttgtcatcgc tgtcagtctt ctggctggac gtatcgtgta
      241 cacgctcgta agcggcccta acggcccgct aacgcggaga tacgccccga cttcgggtaa
      301 accctcgtcg ggaccactcc gaccgcgcac ataagctctc tcatggctga cagcgggcat
      361 ggcttagcag ggcggggatg ggtaaggtga aatctatcaa tcagtaccgg cttacgccgg
      421 gcttcggcgg ttttactctt gtgccatatg tagctacatt ataccgcctt ccatgccgct
      481 cacgcggcat cagaacaata aaaccagcat taaatgtatt tgcaaaagta tgagtaatac
      541 agacttgtta accggtcctt tcagaggtat ctttaagact tgttaaacag tctcaaaggt
      601 acgttatgcc taacatcatt ctgtctgaca ccagtgcaag tgtcagtgaa ctgaaaaaaa
      661 atcctatggc tacagtcagt gccggagatg gttatcctgt cgccattctt aaccggaatc
      721 agccggcatt ctactgtgtg cctgcggaac tctatgaaag gatgcttgat gcgctggatg
      781 accaggaact ggtgaaactg gttactgaac gcagcaatca gccactgcat gatgtggacc
      841 tggacagtta tttatgactt atacagtaaa attcagggat gatgcgctca aagaatggct
      901 gaaactggat aaatccattc aacagcagtt tgcgaaaaaa ctaaagaaat gcagcgaaaa
      961 tcctcatatt ccttccgcaa aactgcgtgg attaaaagac tgctacaaaa tcaaacttcg
     1021 cgcatcgggg tttcgtctgg tctatcaggt tattgatgac atgctaatta ttgcggttgt
     1081 cgcggtaggt aaacgtgaac gtagtaacgt ttataatctc gccagtgaga gaatgagata
     1141 gtt
//
LOCUS       DAJOTB010000351         1134 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJOTB010000351 DAJOTB010000000
VERSION     DAJOTB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.362"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          326 a          218 c          206 g          384 t
ORIGIN      
        1 ttattcttat taacttattc ttatcgggta tcagttttga taggggtagg ggtatcagac
       61 ttgatagggg tagtcatatc aaagttaata ggggtatcaa tattgatagg ggtatcatcg
      121 gtttattatt ctttataaac ccctgttaga cttgataggg gtaacagaat tctgtggggg
      181 tagctcacag tgacctaccg ttacatccat ttgtcacata gcactctgtg taatgccttt
      241 ctatgtgtgt gccataatcc cctatattcc ctgtgtaaat catagaataa tgctctctat
      301 tccctgtgta attccatact aaaaattcaa tgaaaaagta gcttaaaaga gcaatgttaa
      361 ctttcttcac ttttacatca ctttcgcaaa gccttgtgta gtgcggctta cagagcacca
      421 tcagaaattg ttggctaaaa attgttctgc ttgtttctta atgagttttc ttgtttgtgc
      481 aaattttaaa atttattttg tttttacagc cttatttcgt gtaatgcgtg gcatagcaag
      541 cccttttctt gtgttgtcaa gacatttggc aacgttcaaa catctgttta gaaatgccaa
      601 attgttgatc taacaaataa acctgtataa acagaacacc atttaatcta tccccctgtg
      661 taatccacta catcacacga taaaaacaaa aaacctttaa ttatatttca tagcacacaa
      721 aagtcaacag aagccctctc gtgctcctac gtaaaaaatc catcactttt cccctgatac
      781 atcaattcaa ggcgttctat gtgttcctta gtgcatatta cgattattat gttaaatgga
      841 ttttggccta tattttgacc tgcacccgca taaacactgg tttcatcgct tacagccctt
      901 gtgagacgtg gtgctgtttc tagcatctga tccgacataa aaccgatcat tctcgtgttt
      961 agtgaaagtt tagtgagaga cattacagaa aggctttctg tgtaatgatt taataggtaa
     1021 aaacgatgtt gattacgcaa gaaaattcct tgctgatatt tggggattct ggattttcca
     1081 gatcgggggg cgctgaaaat cgcgttaagc acctctcgca atctagccat aagg
//
LOCUS       DAJOTB010000352         1133 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJOTB010000352 DAJOTB010000000
VERSION     DAJOTB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.363"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(57..392)
                     /gene="ygaH"
                     /locus_tag="OHN28_005003"
     CDS             complement(57..392)
                     /gene="ygaH"
                     /locus_tag="OHN28_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCQ2300233.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(382..1119)
                     /gene="ygaZ"
                     /locus_tag="OHN28_005004"
     CDS             complement(382..1119)
                     /gene="ygaZ"
                     /locus_tag="OHN28_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCQ2300234.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
BASE COUNT          325 a          293 c          298 g          217 t
ORIGIN      
        1 taacgacagt gacaaataaa gtaaatgtat tgttttagaa aaatgattct tgtggattat
       61 ataatcgcca tcactttcca ggcgagccca taggccagcg cactaagcag tgttgggatg
      121 ataatgctgc gtgttttata gaaactggca cccagtaccg cgaagccgac cagtgtgggc
      181 acgaaacggc gtgtatcgtg catcacttct ggtgcggtag agacaaccag cagagcgcat
      241 atcgaggcga tgccaatggt gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg
      301 gcattaccca cacgcaggcg cagcggcaga tagcggaagc aataattcgc cgcgccaacc
      361 agtaacccaa gcagcagaac ctcatagctc atcgggcgct ccttgccaga atgcctggat
      421 taacgcagtg aggcagccac agacaatgcc tgccagaatg gcgacgggaa tagaaaatag
      481 cgttacgcct gcaagggcac caactaacgc tgcggtaacg caaagagatt gtttgcgctg
      541 gaaagaggcg agcaggaaac tcataaagag tgccggaagc ataaaaccta atgctgcttc
      601 aacggcggga taaccttgca gcaagccgct gccggagaat gcccctatta ccgtaccaaa
      661 tacccacgat gaccatgaac tgaaggcaat gccgatcatc cagttttcgc tccagcggcg
      721 attattgcgt accagttttg cggttgcggc ggcaaaaacc tcatccgtca ggccaaacgc
      781 ccacagggcg gttttcgatt tttgcagacg ctgaataata cggctacgca gtgacgggcc
      841 atacaaaaca tggcgaacat ccattgccat gacggtcagt gcagcaaccc acaaactact
      901 cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg cctgcataaa tgatgcagga
      961 gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc gcattcagac cgaacgcaaa
     1021 ggccaccgga atataactaa taacaatcgg taaactgtct ttgcatcctt ccatgaaggt
     1081 cgccgaacca ggagcaggct gtggagtagg gctttccata gagtgtacgc tta
//
LOCUS       DAJOTB010000353         1127 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJOTB010000353 DAJOTB010000000
VERSION     DAJOTB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.364"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..726
                     /gene="sdiA"
                     /locus_tag="OHN28_005005"
     CDS             4..726
                     /gene="sdiA"
                     /locus_tag="OHN28_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCQ2300235.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(794..1018)
                     /locus_tag="OHN28_005006"
     CDS             complement(794..1018)
                     /locus_tag="OHN28_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCQ2300236.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1005..1127)
                     /locus_tag="OHN28_005007"
     CDS             complement(1005..1127)
                     /locus_tag="OHN28_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300237.1"
                     /translation="MTLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          292 a          244 c          273 g          318 t
ORIGIN      
        1 actatgcagg atacggattt tttcagctgg cttcgcacga tgctgttgcg ttttcagagg
       61 atggaggccg cagaagaggt ctaccatgaa atagagcttc aggctcagca gctggagtac
      121 gattactatt cgttatgtgt tcgccacccg gtaccattca cgcgacctaa agtggctttc
      181 tacaccaatt accctgagtc gtgggttagt tattatcagg caaaaaattt tctcgcaatt
      241 gatccggtgc tgaaccctga aaactttagt cagggccatt taatgtggaa tgatgactta
      301 ttcagcgaag cacagccgtt atgggaagct gcgcgcgcac atggtttacg ccgcggtgtc
      361 actcagtatt taatgctgcc aaatcgggcg ttgggctttt tgtccttttc ccgttgcagc
      421 acgcgcgaaa tacccattct tagtgatgaa ctgcaattaa aaatgcagct actggtgcgc
      481 gaaagtctga tggctctgat gcgtttaaat gatgaaatag tgatgacgcc agagatgaat
      541 ttcagcaagc gcgaaaaaga aattctgaaa tggacggcgg aaggtaagac atcagcagag
      601 atagcgatga ttttgtcaat ctctgagaat acggtcaatt tccatcagaa aaacatgcag
      661 aaaaaaatca atgcaccgaa taagacgcag gttgcctgtt acgcggccgc tactggctta
      721 atttgatctc ttttctgttc tgcgtgccag atgcaaaaac cggctgaaag gcacgctctc
      781 agccggtttt atattactga cggtaggctt gtttaatttg cttaaccgtt ttggaaaata
      841 ctgcagcctg ggtttcgtct tcgatcagcg caagctgttt ttccatcttt aacatcacgc
      901 ggcccgcgtc agcttgtccc atcgcctgca gcatcagcgt cagcatcgct ttcaggcagg
      961 agacttcatt tgccagttct tgattattct cggcagtaga aaaatcaggc gtactcattt
     1021 attttcctcg tcatgttgca atgaaaattt gcggtgaaaa atgttaaggc ggcggagtat
     1081 accataagct ttgctaaaaa tagcagtggt tgttttttga gcgtgat
//
LOCUS       DAJOTB010000354         1098 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJOTB010000354 DAJOTB010000000
VERSION     DAJOTB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.365"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..200
                     /locus_tag="OHN28_005008"
     CDS             <1..200
                     /locus_tag="OHN28_005008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300238.1"
                     /translation="VDIIRHSNFEASEDDNAIEYHYAACLRLYRLLGDEAEDILVEEL
                     LQHPTITILRAIGFLVGHEIK"
     gene            550..1098
                     /locus_tag="OHN28_005009"
     CDS             550..1098
                     /locus_tag="OHN28_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300239.1"
                     /translation="MKTYHLSSANRSAEHYIMFSYGVSLRLSNYLFNSKPVARDFFKH
                     LKGTTQRGLTDKHLDNLDYFKGLGAYHIVSKRFVEAMKSYLKEDVTFHAMQVSGRNTS
                     HDLYLLQVNKTLPIIKETSFDLNNDRVFSPPIFKKNHDISFLIAREENVLMNHIVIVS
                     DEFVSLCARHQLKINFRQTLTE"
BASE COUNT          384 a          175 c          191 g          348 t
ORIGIN      
        1 tagttgatat aatacggcat tctaattttg aagcttcaga agatgataat gcaatagagt
       61 accattatgc tgcatgtttg aggctttata gattgcttgg tgatgaagca gaggatatat
      121 tggttgagga attattacaa catccaacaa taaccatatt aagggcaatc ggttttttag
      181 ttggacatga gataaaatga accattaata aaaatacatt ttgtatactt aatgaaatct
      241 ctgaagcata ttccgagcaa ttatctattg tcgcaaaaga cgtattaatt gaagatgttg
      301 cgcgagttaa tgcaataaat caatttctat aaatttacga tatttattag ataattatgc
      361 cgagtattca atttggagat gatcaggcag actatccgtt tttatcggag ggagtcatct
      421 ggctaaggtt gatgtgatat gtccgtttta tcgtcaaggt taaacggaat ataagaaatg
      481 accacctcgc ttcagtcctt atagagagcg actgtcgtaa ccttctccca agtttaaaga
      541 gaaccagata tgaaaactta tcatctaagt tcagcaaaca gaagtgcaga acattatata
      601 atgttctcat atggtgtatc attacgacta tctaattacc tttttaacag taaaccggtt
      661 gcaagagatt tttttaaaca tttaaaagga actacgcagc gaggtttaac agataagcat
      721 ttagacaatt tagattactt caagggctta ggagcttatc acattgtatc taaacgcttc
      781 gtcgaagcaa tgaaatcata tttaaaggaa gatgtaactt ttcatgcaat gcaagtctca
      841 ggaaggaata cttctcacga cttatacctt ttacaagtca ataaaacttt gccaataatt
      901 aaagaaacat ctttcgattt aaacaatgat cgtgtatttt cgccacccat tttcaaaaaa
      961 aatcatgaca tatcatttct aatagccaga gaagagaatg tcttaatgaa tcatattgtc
     1021 atcgtcagcg atgaatttgt ttctttgtgt gcacgacatc aattaaaaat aaacttccga
     1081 caaacactta cggaataa
//
LOCUS       DAJOTB010000355         1063 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJOTB010000355 DAJOTB010000000
VERSION     DAJOTB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.366"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            60..>1063
                     /gene="waaB"
                     /locus_tag="OHN28_005010"
                     /gene_synonym="rfaB"
     CDS             60..>1063
                     /gene="waaB"
                     /locus_tag="OHN28_005010"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HCQ2300240.1"
                     /translation="MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDK
                     MDKGWLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKA
                     RKKSGIDVPVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIF
                     NPVEAKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGS
                     DFEKCQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESM
                     SWGIPCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPAS
                     "
BASE COUNT          312 a          174 c          241 g          336 t
ORIGIN      
        1 ttttatagaa aaataaaacc gttaaaacag tgagttttac tcaattaatg agagtttcaa
       61 tgaaaatagc atttatcggc gaagctgttt ctggatttgg cggcatggaa acggtcattc
      121 gtgatgttat tactaccttc aggcaacaac atatctatag tgaaatgttt ttcttttgtc
      181 gtaatgataa aatggataag ggatggctgg aaggtattaa gtactcgtgt tcattttcca
      241 atattcgctt aggttttttg cgtcgggcga aacatattca tgctttaagc aaatggcttc
      301 aagattatca gccagatgtt gttatttgta ttgacgttat ctcgtgtttg tatgcggcca
      361 aagcacgtaa aaaatcagga attgatgtgc cagtattttc atggccgcat ttttctttag
      421 atcacaaaaa acatgctgaa tatatcacct gtgcagacta tcatttagcc atcagctctg
      481 gcattaaaca gcaaatgatt aatcggggtg taccagaatc gacgataaat gttattttta
      541 atccagtcga agctaaagac acagtcattc ctgggcctga ggaaggtgaa acggcaacgt
      601 tcatttatgt tgggcgtatg aaattcgagg gccagaagag ggttaaagac ctgcttgatg
      661 gtttatcaca agcgcaaggt gactggaaac tccatgtact gggtgatggt tctgattttg
      721 aaaaatgcca ggcatatggt agagaattga agattgataa tcgaattgtc tggcatggtt
      781 ggcaaaaaca tccatgggag gtagtgcgtc aggatattaa gaaagtcagt gcattgttat
      841 taacctcatc ttttgaaggt tttccaatga ccttacttga atcgatgtca tgggggattc
      901 cgtgtatcag tgccgattgt gtctccggtc ctggtgacat tattcaacct gatgtcaatg
      961 gccatttgta tcaaccggga gatattgctg gttttgttac tcttctaaat aaatatatag
     1021 caggtgaaat tcatatcgaa catgaaaaaa ttcctgcgtc gat
//
LOCUS       DAJOTB010000356         1017 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJOTB010000356 DAJOTB010000000
VERSION     DAJOTB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.368"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(589..822)
                     /locus_tag="OHN28_005011"
     CDS             complement(589..822)
                     /locus_tag="OHN28_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCQ2300241.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(833..1006)
                     /locus_tag="OHN28_005012"
     CDS             complement(833..1006)
                     /locus_tag="OHN28_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300242.1"
                     /translation="MESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDELMAKI
                     RTHEYNRSMDVIS"
BASE COUNT          300 a          264 c          180 g          273 t
ORIGIN      
        1 tctcaatgaa tatctttgat gaaatgcacc gatcagcaag atataagcaa ctaaaaatag
       61 tattaatact gataaaaaag cacgaattat gcttacattt gacatgaaat taactataaa
      121 ccaccatttt tcaaacggtg aaaacggaat gctccccatg ataataagta gaagaacaaa
      181 gatacaactc cataaaaccc acaatctgta cataagaaag atcctttttc tttttataat
      241 actgcaacta cacctcaagt caaaaccctc ttctgatatt accctctact acagtaaccc
      301 atcacatgac ctccgactta tcagaagact agccaaactt tctcaatcaa atcaggttgc
      361 gcgcgctcgt atccccgcca cgcctgcccg ctttatgtag tggttttcat gcacctgcat
      421 gatctacgca aaagcccgcc agttctggcg ggccttagca aaaacgatcc tcaaacgatc
      481 atgcgatctc atgcggcata gacatgcact acagagctaa cgcctcgcaa gggctcgttg
      541 ttcaaccttg ttgacgccag aagcaagttc agacgccagc aacgtttctt aatgcagcca
      601 gctgtcgtct tcccacacct tctgcataat tttcatcact tgttttcttt cttcgtccag
      661 ttgcagtccg gttagttcca caccgttaga gctacctttg cgaatgcgaa ttaccgtttt
      721 gggatacagg gggcgcagat tgcggtaaag ctcggattca agggcgtcca gggtagactg
      781 gctaatcttc tgctctttat cgatcattat ttcaatgcgc ataaaagtca cctcagctga
      841 tgacatccat tgagcggttg tattcgtggg ttctgatttt tgccatgagt tcatcagtca
      901 attcagaaac ccactgcaga gccagcccct tctcttcatc actacactca ctagccgcta
      961 caagcttaag aaaaaaatca atgcgctgga gcttcaaaga ctccaaaaaa tagtcct
//
LOCUS       DAJOTB010000357          983 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJOTB010000357 DAJOTB010000000
VERSION     DAJOTB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.369"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(489..686)
                     /locus_tag="OHN28_005013"
     CDS             complement(489..686)
                     /locus_tag="OHN28_005013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300243.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVGLGML
                     FLMIMIAGGMCGSLLSRKKTS"
BASE COUNT          323 a          220 c          194 g          246 t
ORIGIN      
        1 taataaatgc gataacataa acaacataca tcctgaacat tcttattact tcatcaggat
       61 ctgtctgaag gatactgatt gcaaaaccat cattaaatgt tgtgccaaat gtacaccatg
      121 aatacaggct gatactgata tctgcagcag tcagtaatgt gaacgggaag gcaagaatca
      181 aacgcaacgt aatcgatttc attgccttaa taataagaag tgccaggata aagaataata
      241 tccggtaaat cagatgcgct cctgaggata caaggagatt agaaagggta aagacaacca
      301 ctgataacac gaggcgctta tcattgctaa tccgtgacca gtgtatgaac ggaagattaa
      361 ttttcataat aattgagaaa aagcgatatt tctgtccggc gctggtcgct gaacatgccg
      421 cgacaggtga attcattatt ctgaataatc ataaagccac cacaaatgta gcggcgtaaa
      481 caggaacgtc atgatgtttt ctttcgtgac agaagagaac cgcacatacc acccgcaatc
      541 attatcatca ggaataacat ccccaggcca acgattactc ttaatgccgg atgaacccga
      601 tcggcgagaa aaaataactc catcccgaaa ccaataacaa gccaggccag cgccattccg
      661 acgagccctt ttaacagcaa tctcacgctc ctctccttct gcattcttaa cgaaaatgcc
      721 ttacaataac agcagccggg gaaggcaacg ctggctgctg aacggtatga cattttactg
      781 ctttatgttc tctcagtttc tgtactgacg ccgccattaa tggtattaca gtttattcag
      841 aataaggcat gcggcctggt ttaatgcaat aaactcctgc cagctgtact gagactgata
      901 attcagtaaa tactggtctt caacccggca aatctgtggg cgtttatcat aaattgcaca
      961 caaatgagaa tcagtctgat agt
//
LOCUS       DAJOTB010000358          949 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJOTB010000358 DAJOTB010000000
VERSION     DAJOTB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.370"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(61..306)
                     /gene="dinI"
                     /locus_tag="OHN28_005014"
     CDS             complement(61..306)
                     /gene="dinI"
                     /locus_tag="OHN28_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCQ2300244.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(383..>949)
                     /gene="pyrC"
                     /locus_tag="OHN28_005015"
     CDS             complement(383..>949)
                     /gene="pyrC"
                     /locus_tag="OHN28_005015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCQ2300245.1"
                     /translation="SVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQH
                     LMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKE
                     SSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREE
                     QQVAESIALTDDTLVPFLAGETVRWSVK"
BASE COUNT          244 a          237 c          240 g          228 t
ORIGIN      
        1 caattcgcaa gtaaaataaa aaagccgggg cgacccggca aaaacaaaaa tcactgcata
       61 ttattcgctg acaaaccagt catcggcgct ttcccacgtc tcctggagaa tttcgctaat
      121 gcgctgttta tcttcttttg tcgcgccaat aaccgataaa ttatttgctg cggcataacg
      181 taccgatacg tggccttcat tatcaggaaa cgcatactga atacggcggg aaagttcgcc
      241 agccagggcg tcaatagccc cagctggcaa tggagaagtt ttcgctatgg tgacttcaat
      301 tcgcataata gccccctgtt gaatatactg gttatttata caggtaaaat aacctaatga
      361 caacaggaag ctacgatttt tattatttaa cggaccagcg taccgtttct ccggcgagga
      421 atggcaccag cgtgtcatca gtcagtgcga tgctttcagc aacctgttgc tcttcacgta
      481 ccagttcgat gaatgtgtcg ttgaccggca agccatagaa ctgcgggccg tttacagaac
      541 agaatgcttc aaagtgctgc aaagcattca tctcttcaaa gacggtagcg taactgccca
      601 gcgcggttgg ggcgttgaaa cagcccgcgc agccgcagct gctctctttg cgatgacgtg
      661 catgtggcgc agaatccgtt ccgaggaata cacgattaaa accgctggcg accagttcac
      721 gcaatgcctg ttggtggata ttgcgtttga ggatgggtag acaatacagg tgcggacgca
      781 caccaccaac cagcatatgg ttgcggttaa acatcagatg ctgcggagtg atggtggcag
      841 ccagccgttc atttccgtca cggacatagt cggcagcgtc tttggtggtg atatgctcaa
      901 aaacgacttt cagcgcggtc aggcgctggc gcagcggttc catcacgct
//
LOCUS       DAJOTB010000359          944 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJOTB010000359 DAJOTB010000000
VERSION     DAJOTB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.371"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..496
                     /locus_tag="OHN28_005016"
     CDS             <1..496
                     /locus_tag="OHN28_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HCQ2300246.1"
                     /translation="PFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITF
                     IEKHFTLDKSMSGPDHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVAR
                     KSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHSEFK
                     NQGE"
     gene            496..>944
                     /locus_tag="OHN28_005017"
     CDS             496..>944
                     /locus_tag="OHN28_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCQ2300247.1"
                     /translation="MRTKIIAIIPARSGSKGLRNKNALMLIDKPLLAYTIEAALQSEM
                     FEKVIVTTDSEQYGAIAESYGADFLLRPEELATDKASSFEFIKHALSIYTDYENFALL
                     QPTSPFRDSTHIIEAVKLYQTLEKYQCVVSVTRSNKPSQIIRPLDDYS"
BASE COUNT          338 a          143 c          181 g          282 t
ORIGIN      
        1 gccctttgag gatgtaaacc ttaatgctat taatgatttg aaaaaacact tccctaagaa
       61 taacataggc ttctctgatc attctagcgg gttttatgca gctattgcgg cggtgcctta
      121 tggaataact tttattgaaa aacatttcac tttagataaa tctatgtctg gcccagatca
      181 tttggcctca atagaacctg atgaactgaa acatctatgt attggggtca ggtgtgttga
      241 aaaatcttta ggttcaaata gtaaagtggt tacagcttca gaaaggaaga ataaaatcgt
      301 agcaagaaag tctattatag ctaaaacaga gataaaaaaa ggtgaggttt tttcagaaaa
      361 aaatataaca acaaaaagac ctggtaatgg tatcagtccg atggagtggt ataatttatt
      421 gggtaaaatt gcagagcaag actttattcc agatgaatta ataattcata gcgaattcaa
      481 aaatcagggg gaataatgag aacaaaaatt attgcgataa ttccagcccg tagtggatct
      541 aaagggttga gaaataaaaa tgctttgatg ctgatagata aacctcttct tgcttataca
      601 attgaagctg ccttgcagtc agaaatgttt gagaaagtaa ttgtgacaac tgactccgaa
      661 cagtatggag caatagcaga gtcatatggt gctgattttt tgctgagacc ggaagaacta
      721 gcaactgata aagcatcatc atttgaattt ataaaacatg cgttaagtat atatactgat
      781 tatgagaact ttgctttatt acaaccaact tcacccttta gagattcgac ccatattatt
      841 gaggctgtaa agttatatca aactttagaa aaataccaat gtgttgtttc tgttactaga
      901 agcaataagc catcacaaat aattagacca ttagatgatt actc
//
LOCUS       DAJOTB010000360          938 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJOTB010000360 DAJOTB010000000
VERSION     DAJOTB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.372"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..856)
                     /gene="ompN"
                     /locus_tag="OHN28_005018"
     CDS             complement(<1..856)
                     /gene="ompN"
                     /locus_tag="OHN28_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCQ2300248.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTA"
BASE COUNT          237 a          222 c          208 g          271 t
ORIGIN      
        1 gtgcagtgac ttcaaagttc tgggttttgt ttgcaacagc ataatcgctg tcgccaaacg
       61 gggtcatatt acgcgtttct gaatacatgg ttgccaggta aatattgtta gcatcgtatt
      121 ttagcccagc agtccacgcg tctgctttat caccacccgc cgcagtatgg ttaacctggt
      181 cattggtgcg gtcagaagag gtgtatgccg caccagcgct aaagcccatg cctaaatcat
      241 atgttgtgga aagaccccag ccgtcaccgt tttcatggcg aacatcacgt ccgttgttgg
      301 tgccttcctg accattactg gcaccttcgt tgttaccttg atactgcacc gcgaagttca
      361 gaccatttac cagaccgaag aaatcagtat tacgataagt cgcgacgcca ttggcacgac
      421 cagtcataaa gttgtctgca ttggtataag agtcaccgcc aaattcaggt agcatatcag
      481 tccagccttc gatgtcgtac attacgccat aattacgtcc gtaatcgaaa gaaccgtaat
      541 ctgcaaattt cagtccggca aatgccagac gggtccatga ctggtttgtt gaagattcgg
      601 tgttgtttgc ctgaatattg tattcccatt gaccgtagcc agtgagttga tcgttaattt
      661 gggtttcgcc tttaaaaccc agacgagcat agctctggtc gccatctttc gctgaattat
      721 cagaaaaata atgcaggcca tcaactttgc catacagatc caatttgttg ccgtctttat
      781 tataaacttc ggctgcatgt gcagcacctg cagcgagcag ggcaggaatt aagagtgcca
      841 gtactttgct tttcattgaa taaatccttt agttatttta tttgcctttc atccccattt
      901 cggggagtga aaacatccta aaggagaagt tcagttag
//
LOCUS       DAJOTB010000361          926 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJOTB010000361 DAJOTB010000000
VERSION     DAJOTB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.373"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            18..299
                     /locus_tag="OHN28_005019"
     CDS             18..299
                     /locus_tag="OHN28_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCQ2300249.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LSAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
     gene            324..>926
                     /locus_tag="OHN28_005020"
     CDS             324..>926
                     /locus_tag="OHN28_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300250.1"
                     /translation="MRYLTELICKLYRLTDHHKLTTATFKNLADIKLFKPTADADTIL
                     QLENIFSEYSLRYIDRDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVI
                     NGSWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRNPINVKYYSWFDRY
                     VASNSGGSHHAAMVVYQSVRDGLGYKREAIIKQLSIDPGTV"
BASE COUNT          315 a          175 c          197 g          239 t
ORIGIN      
        1 tacaagggga aattgtcatg gctttaaaac taaaccgtcc gaacatcgat gaatctcagc
       61 aaccagcaac aaacgcagaa accgcgcgtt ttatctccgg cgctaccaga gcgcctgtag
      121 tgggtaaacc aaagttgatg aacttcagac tttcagccca ttttgaggac atactggaag
      181 ctgaagctat cagaacaggg cagagcaaaa caaccgttct caaagcggcg ctggcgatgt
      241 acaacagcca ggatgaaaac ataaagaatc actggttgct tgagtctgca aaattctaac
      301 catagaagca tggatgaaaa tctatgagat atttaaccga attgatctgt aagctctaca
      361 ggttgacaga tcaccataaa ctgaccacag caacgtttaa aaatcttgct gatattaagt
      421 tatttaaacc tacagcagat gcagacacca tattgcagct tgaaaatata tttagcgaat
      481 actcactgag atatattgac agggatctgg ctgaaattct gagcaactta ataactacag
      541 ataagaaaca ctctctggac ttcgatttta acaaaataca atctctgact gactcgaaaa
      601 gtttcggttg tggatggagc aaggtaataa atggtagttg gttcaaagat ctttattcgt
      661 ggggagaggg gatgtatcct gccgaaaatc tgaaagctga aaatatttta gactggaaag
      721 ataatatatg gcacattgag catgaaggtt tccccccacg caaccctatt aatgttaaat
      781 attattcttg gtttgaccga tatgtagctt caaacagtgg tggttcacat catgcagcaa
      841 tggttgtata tcaatcagtc agagatgggt tagggtataa aagagaagct attattaaac
      901 aactgagtat agacccaggc actgtt
//
LOCUS       DAJOTB010000362          922 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJOTB010000362 DAJOTB010000000
VERSION     DAJOTB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.374"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..770)
                     /locus_tag="OHN28_005021"
     CDS             complement(<1..770)
                     /locus_tag="OHN28_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCQ2300251.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPV"
BASE COUNT          242 a          226 c          217 g          237 t
ORIGIN      
        1 aacacgggct gaccatgttt atcgtagtag tgcaggcaga tatcgcccac agcaccgcga
       61 tccgctagta cctgtaacat atcctcgtga taatagttgc ccgaggtttt tagcaactgc
      121 gagggttcaa ggataccgat gccgacaatg gcgatatcga cgtcgtcaaa tctggcgata
      181 acgtcagcca catcctggct tgccaccagg cgttttttct cttctaccga accttcaatg
      241 ctttgagaag gaagcaacca ggcttcgcaa ttcagatgct gggccagggt ctgtgtcaaa
      301 atagttgcct gcacattgcc gtttggcccc acgccgccca gcagctgaat aacgccgctg
      361 gcttttaagt tttgggcgtg aacttcatcg accatcgcgc gaatcgtaga actccacgaa
      421 gagacgccaa tgaaatcttt tggccttaaa cgcgtttcca gatagtgcgc ggcggcagag
      481 ccgatggcgc gtttaatggt gtgatcactg gcatcatctt cggtatcgac aacaatcgct
      541 tgtttaatgc cgtaacgctc ttcaatgcct ttttccagat tgagaaagat atttgacggc
      601 tggacaacgc tgattttaac cacgccttct ttctggcaac gggttagcgc tctggatata
      661 aatgactgag aaagcgagag aagttgggcg atgtcagact gttttcgccc ctcaagatag
      721 taaagagtgg cgatcttcac cagtagccgc tgttcatcct gcttagccat atatttcccc
      781 gtaaatcatt aacgtaccgc ccttatctta gaggctttta tcatacgcga aaaaagaaga
      841 cagttaagtg tgatggagtt ctaactttaa ccaatcctta aaatcagcga tggacaaaaa
      901 taaaaaacag gcggcataat tg
//
LOCUS       DAJOTB010000363          876 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJOTB010000363 DAJOTB010000000
VERSION     DAJOTB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.375"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            100..510
                     /locus_tag="OHN28_005022"
     CDS             100..510
                     /locus_tag="OHN28_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300252.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEYIALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQILDNADHYGDF"
     gene            512..847
                     /locus_tag="OHN28_005023"
     CDS             512..847
                     /locus_tag="OHN28_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300253.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPAALNEAF
                     KHWDLAVNAFEHLSKRVEMLCKSENAYLTADQVFELSNWIEDIESDVRYVGDGLVELA
                     QRLGAITEE"
BASE COUNT          309 a          153 c          168 g          246 t
ORIGIN      
        1 acattattat gatagtagct atgtattggt tattgttctt tatcacgtta gaagcatatt
       61 tcggggtgtt ttcctttaaa agaaaaggaa aagtaaataa tggcaaaaag aaaaaacaac
      121 gttgttaaga aaatcggtga ttcagcttct cttctaagta aacctaaatc tattctaaga
      181 cgtaaagact ttaaagagct agttacgctc accaagcaaa atagcgcccc tggcgaatgg
      241 aaaacagaga tcatagaaca ttctccctct gtgccttgtg gagatgaata tatcgcatta
      301 caagaaatct tatcttcaac acctggcgta ttctggaaac ctagaaaaag aaaagagtat
      361 attgttgata gctctgattt acgcaaatac cagattttag gatttgaaga ttataaccac
      421 tacgtcggtt atctcgccac aaatggccta aataatttag ttccagaatt ccaaatttta
      481 gataatgctg atcactatgg agacttttaa tatgaacaaa aacacgtatg acacaattta
      541 ctcactaatt aattattatg aggatgatta cctcctgcct ttaaaccgtg ctgaacttga
      601 agcatacaaa gaaaacacgc cagcggcact aaatgaggcg tttaaacact gggatttagc
      661 tgtgaatgcc tttgagcacc tctctaagcg tgtagagatg ctctgtaaga gtgagaacgc
      721 atatctgaca gcggatcagg tattcgagct atcaaattgg atagaagata ttgaatctga
      781 tgttcgctat gtgggagacg gacttgttga actagctcaa cgcttaggcg ctatcacaga
      841 agaataaaaa aagcaacaaa aaagttgtaa aatatc
//
LOCUS       DAJOTB010000364          864 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJOTB010000364 DAJOTB010000000
VERSION     DAJOTB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.376"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..773)
                     /locus_tag="OHN28_005024"
     CDS             complement(<1..773)
                     /locus_tag="OHN28_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCQ2300254.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     DSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDG"
BASE COUNT          217 a          189 c          195 g          263 t
ORIGIN      
        1 ataccgtcct gaataacttt cccgttatta ccggcgatct ccgtgccagt ggaatcttta
       61 ccatcaacga tggttttgcc attgttgttt actgtggcat tatcacccgt aataacaagc
      121 tcaccgttat gattatattg ccacttcgtt gcgccatctg cgctcacccc gtcaagggca
      181 accgtgttat tcgcttcatc aatttgccaa atattaatag ttgccttacg gccattgcta
      241 tcttgtagag aaatagtccc gtcagcattc tcggtataag taaaaactga atcgcgaata
      301 gttaacgttt ttgctgtttt atcgaggata acgtcattat taaacgaaat tggtttggga
      361 ggcgtcgggg tatcatccga accgtcgtct ggtattatct catctccgcc aggagttggg
      421 ggaactggag tgtcgtctga attatcatca ggtggagttg gtggaagtgg agaattattg
      481 cgatgatgat gatcatcatc gtcgtttagt tccactatgg ctaaggctgt gattaacgca
      541 gtagcagcac ctgtggcaag ccatggcatc aggttctgtt cgactacagg ggagcattca
      601 gagggtaagc gtttaagctc ttctggactg agttttccag ggtctgccgg acaagtcaca
      661 ttggtatttt tcttttcacc tgtggtggtt tctatctctg tgatatctgc cgcccagccc
      721 tgaccactca gagccaatgc aatacaggct gacaatagag ttttcctttg cataataaat
      781 ccttctggcg aatataaaga aggtcggatg ccttaaccca accaattaat caatcaatta
      841 aaacatagag gacattcaat attg
//
LOCUS       DAJOTB010000365          864 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJOTB010000365 DAJOTB010000000
VERSION     DAJOTB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.377"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            259..>864
                     /locus_tag="OHN28_005025"
     CDS             259..>864
                     /locus_tag="OHN28_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2300255.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSRE
                     ELSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQSS
                     GHTKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLIS
                     SVRLADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELF"
BASE COUNT          259 a          145 c          171 g          289 t
ORIGIN      
        1 tacgcatttt tctgttttta ttcagatatt cgtatctttg tatcttcatt tctttagttc
       61 tttatagtgc tctaatctaa cgtatgacgt taaaaggtgg atacctgcgc atttctatca
      121 tattgaatat aaatgataaa aattctttca tgtgatccgt gttttggtgg aaatatataa
      181 ggtggataaa ccgctttaca tgtttcctcc tttgtggtta tgatttcacc atcagaactt
      241 aaaaaccaca ggtaatgtat gaacttactg agcgttacaa agaaaacaaa aattcgccac
      301 agaaatgaaa ttaacagcac attctcatcg ttgcctttgt ctgccagaag aattttgttt
      361 atggctatgg cgcagattga ttcacgtgaa gagttatctg agggggcaac ctttcgtatt
      421 acggctcggg aatatgcatt tatcgctgat attgatgtaa caggtgctta ccggcagttg
      481 aaagaggggg cagaagaact tcaggccagt gttattcgta ttcctcgaaa tcaacttctc
      541 acagctcagg gcaatcaaag ctctggacat acgaagaaga gaggtaaatt accttctgat
      601 gcagtcaggc ttatgaacct gactgcgttt tgtgattatg ttgaaagtga aggatatatt
      661 gatattgctt tttcacatgt cattgagcca tatatcacga tgttaaagga ttcttacact
      721 actcaggttt tgatatcgtc tgtaagactc gctgatcaaa attcaaatat gctttatcag
      781 tttatcagga aacaatacag cagtggtaaa aagacatttt tcgacattga agttgatgta
      841 ttaaaggatg aacttgaatt attc
//
LOCUS       DAJOTB010000366          863 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJOTB010000366 DAJOTB010000000
VERSION     DAJOTB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.378"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..702
                     /locus_tag="OHN28_005026"
     CDS             <1..702
                     /locus_tag="OHN28_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2300256.1"
                     /translation="ARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLENRATERH
                     IRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKTRIVHPYKLTNQRGIWYL
                     LATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDVVLFIYSN
                     IAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIVPLILYWLPNVEIIEPVWLKEA
                     VLTMLGKYLTAENVS"
     gene            751..>863
                     /locus_tag="OHN28_005027"
     CDS             751..>863
                     /locus_tag="OHN28_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCQ2300257.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILE"
BASE COUNT          286 a          150 c          194 g          233 t
ORIGIN      
        1 gcaagagcag gaaaccagag caaagaatca ctgcacaaat ctctggccag gttactggat
       61 actgatgcga tttttcctga acgggatgaa ggtttctggc agaaattaga aaacagggca
      121 accgaaaggc atattcgtgt tcagtttcat aaccctgagc acaccatcag ggatgattta
      181 agaaaatatc ttgatgttct tgaaaaagcg atctgcaaca gtaatgtttg ccagattgca
      241 tatgccggta agaccagaat cgttcatccc tataaattaa ctaatcagag aggtatatgg
      301 tatttgctgg ctactgaaga gaacaagtta aagtcgtttt cattagctaa aataaaatgg
      361 ctggatataa aaaaagaaaa gtttgcaaag agtgatgaaa tccagtcact ggtaagtgag
      421 tcatgtgacc cgtgggtgag cgacaaaaca tttgatgttg tgttgttcat ttacagtaat
      481 atcgcccatt attttttgcg acgggactta cttccttacc agcaattgct acacaaacat
      541 gacaatggca ttacgctgtc atgcaaggct gcacataaaa atcaaattgt cccgttgatt
      601 ttatactggc taccgaatgt tgaaattatt gaaccagtat ggctgaaaga agccgtgtta
      661 acaatgttag gaaaatacct tactgctgag aatgtttcct gaaatatttc agttgactga
      721 tgataaagca atctgtacag gaggaaaaga atggcgttac cttttattat tttgggtgtg
      781 gctgccggcg ctgcggcatt cggcggaaaa aaagcttacg atggatatca aaaaaagtca
      841 gaagcagagg atatcctcga aaa
//
LOCUS       DAJOTB010000367          861 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJOTB010000367 DAJOTB010000000
VERSION     DAJOTB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.379"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..279
                     /locus_tag="OHN28_005028"
     CDS             <1..279
                     /locus_tag="OHN28_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2300258.1"
                     /translation="QYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYAD
                     VGATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            420..854
                     /gene="uspF"
                     /locus_tag="OHN28_005029"
     CDS             420..854
                     /gene="uspF"
                     /locus_tag="OHN28_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCQ2300259.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          218 a          207 c          193 g          243 t
ORIGIN      
        1 cagtaccagt ttgattttgg tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt
       61 gacctgcacg ctgccggtgg tgcagacaat ccggcaggtg ttgatgataa agatctggtt
      121 aaatacgccg atgttggcgc gacttactat ttcaataaaa acatgtccac ctatgttgac
      181 tataaaatca acctgttgga tgaagatgac agtttctacg ctgccaacgg catctctacc
      241 gatgatattg tcgctttagg tctggtttat cagttctaaa tccccctgcc cgctgttttg
      301 gcgggctttt tctgcttatt cttcctcctt tgatctaaat taaaaatgtg aactccgtca
      361 ttacacaaaa agtgtcatct ggcgttacac tttatgcgga tactaaaaca ggaggtttta
      421 tgaacagaac gattcttgtc cctatcgata tttccgattc agaattaact caacgcgtga
      481 ttagccacgt tgaggcagag gcaaagattg atgatgcaga ggttcatttc ctgacggtaa
      541 taccttcgct gccctactat gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa
      601 tggatgacct gaaagcggaa gccaaatcgc aactggaaga gatcattaaa aaatttaaac
      661 tgccaaccga cagagtgcat gtccatgttg aggaaggctc gcccaaagac cgcattctgg
      721 aattggcgaa gaagatcccc gctcatatga tcatcattgc ttcccatcga ccggatatca
      781 ccacttatct gctcggttct aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc
      841 tggttgtgcg ctgacactaa c
//
LOCUS       DAJOTB010000368          838 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJOTB010000368 DAJOTB010000000
VERSION     DAJOTB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.380"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>838
                     /locus_tag="OHN28_005030"
     CDS             <1..>838
                     /locus_tag="OHN28_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300260.1"
                     /translation="LSLILRKEDKKLLSLSVQPKELDWLINTVLQNLAKSYSKFATFL
                     NPIEGKLINALKLLSLMKITTEQDAVVLKTLNDILKSSYHNLAFYDAISEYVVLRYNT
                     QSETLSTDSIKTLIYTILDKLISRNLGGYEVIAIVNRGLANIFSVAKKLGVNIEDDSK
                     IDKLLHEISSYPNTDKARAAETILYDLYRISTEKNRDKIKSFIKNISTTDFNEERKIK
                     FELFLLASEISDNYDNLPEKVSKLVENYKGFRFNSEAETIRNLLRYIVNTRKLSDFSQ
                     AL"
BASE COUNT          331 a          142 c          128 g          237 t
ORIGIN      
        1 ccctatctct tatcctaagg aaagaagata aaaaactatt atcgctgtct gttcaaccca
       61 aagaattaga ttggttaata aatactgtac tgcaaaacct agcgaagtca tatagcaagt
      121 tcgctacgtt tctcaaccct atagaaggaa agttaatcaa cgcattaaaa ctactatctt
      181 taatgaaaat aaccacagag caagatgcgg tagtattaaa aacattaaac gacattttaa
      241 agtcctcata ccacaaccta gcattctatg atgctatttc cgaatatgtc gttttaaggt
      301 acaacaccca aagtgaaact ttatccactg acagcataaa aactttaata tatacgattc
      361 tcgataagtt aataagtaga aaccttggtg ggtatgaggt gatagctata gtcaacagag
      421 gccttgctaa tattttttca gtagccaaaa aactgggcgt taatatagaa gacgacagca
      481 agatcgataa gttacttcat gaaattagtt cttatccgaa tacagataaa gcaagagctg
      541 ctgaaacaat actatatgac ctgtacagaa tatcaacgga aaagaataga gataaaataa
      601 aatcatttat caaaaatatt tctacaactg atttcaatga agaaagaaag attaaatttg
      661 aattattttt attagcatca gaaatttcag acaactatga caatcttcct gagaaagtat
      721 ccaagcttgt tgaaaactat aaaggattta gatttaactc agaagcagag acaatcagaa
      781 atttattacg ctatatagtc aacacacgga aattaagtga tttctcacaa gcactttt
//
LOCUS       DAJOTB010000369          835 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJOTB010000369 DAJOTB010000000
VERSION     DAJOTB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.381"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            50..613
                     /gene="fimA"
                     /locus_tag="OHN28_005031"
     CDS             50..613
                     /gene="fimA"
                     /locus_tag="OHN28_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2300261.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          227 a          159 c          191 g          258 t
ORIGIN      
        1 attgttaact ctctttattc agctacttaa atataaattt tggagaatta tgaaactcaa
       61 acatgtcggt attattgtcg tttctgtgtt ggcgatgtcg tctgctgcgg taagcgcagc
      121 cgagggtgat gaatcagtaa cgaccactgt taatggcggt gtaattcatt ttaaaggtga
      181 agtggtaaat gccgcttgtg cgattgattc agaatcaatg aaccaaacgg ttgagctggg
      241 tcaggttcgt tcttctcgcc tggctaaagc gggtgacctc agctccgccg ttggcttcaa
      301 tatcaagctg aatgattgtg ataccaatgt ttccagtaat gcagctgttg cattcctggg
      361 tactactgtc accagtaatg acgatacatt agcgctgcag agttcagcgg caggctctgc
      421 ccaaaatgtc ggtatccaaa ttttggaccg tacaggggag gtattagtac ttgatggtgc
      481 tacttttagc gctaaaactg acttgattga tggcacgaat atactgccgt tccaggctcg
      541 ttatattgct ctcgggcagt cagtagctgg tactgcaaat gctgatgcga ccttcaaagt
      601 tcaatatcta taattcactc ttaaggtgac tatgcgtcat ctgaaattga aacagggtgt
      661 gtgtggcatg gctacgcaca cccgagaaat tcaccaatgt tcatctcatt attaatgaaa
      721 aatggtttgt attttggagc ggtatgaaaa atcacataat atctaaacga tcaataaaca
      781 acagtagtta aacttgctgt tatttaaaat atttacttgg tagcaaggtt ttcct
//
LOCUS       DAJOTB010000370          826 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJOTB010000370 DAJOTB010000000
VERSION     DAJOTB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.382"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            195..812
                     /locus_tag="OHN28_005032"
     CDS             195..812
                     /locus_tag="OHN28_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HCQ2300262.1"
                     /translation="MHKTFLSYHHANEQDLKDEIIDLFGGESFIDKSVSDGDISTTNT
                     DETIMRTIREDFLKDSTVTVVLIGTETKNRPFVNSEIQASLWGENYNGLIGVIRDEIY
                     DSVFTPAVCADPSCGCSISLRTPGLGYDYYLPYLVRKNHVYEKTVPHYNDSDVYCALV
                     KYSVFIKNPEYYIDQAFNKRKLMEPAAKRNPSDVPAIRSSSIFNW"
BASE COUNT          280 a          124 c          162 g          260 t
ORIGIN      
        1 aaagtgacca caaaggatag agaccctaac ggcgaattta tatcattgcc gtatcattta
       61 acccctataa gtactataca aaagcattat tatcatttta gtcaagtatt ggaaggtttt
      121 aaagaagtaa acgatttctt caatgataag gtagtcaaag agtttgatat aaatgttgat
      181 aaatggagtg aataatgcat aagacgtttc ttagttatca tcatgctaac gaacaagatc
      241 tcaaagatga gattattgat ttattcggag gagaatcgtt tattgataag tcggtaagtg
      301 atggtgacat tagtacaact aacactgatg aaacaataat gaggactatt cgtgaggatt
      361 ttttaaaaga ctcaacggtt actgtcgttc ttattggaac ggaaaccaaa aatcgtccat
      421 ttgtaaactc tgaaattcaa gcatcactct ggggagaaaa ctataatggt ctaattggtg
      481 ttatcagaga tgaaatttac gacagtgtgt ttactcctgc agtatgtgca gatccatcat
      541 gcggatgttc tataagtcta agaacgccag gtttgggata tgattattat ttgccttatc
      601 tggttagaaa gaatcatgtt tatgaaaaaa cagttcccca ttataatgac tctgacgtat
      661 attgtgcatt ggtaaaatac tctgtcttta ttaaaaatcc tgagtattat attgatcagg
      721 catttaacaa gcgtaagttg atggaaccag ctgctaaacg aaatccatcg gatgttcctg
      781 caattcgcag ctcatcaatt tttaactggt aataagaggg tgcata
//
LOCUS       DAJOTB010000371          821 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJOTB010000371 DAJOTB010000000
VERSION     DAJOTB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.383"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(247..813)
                     /locus_tag="OHN28_005033"
     CDS             complement(247..813)
                     /locus_tag="OHN28_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2300263.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT          200 a          200 c          202 g          219 t
ORIGIN      
        1 tttttgaaaa cttaccgaga tcaattttcg tgtcgccctt taactcaagg aaacgggggt
       61 caaattgaat ggaatcatca gcccatgcat tgactgatcc accagagatt gccagcgcaa
      121 tatagcaggc gattccccgc agccgaaaat ttgagtgatt aggcatacac atatccgttt
      181 gtgaaacagt cgtaaatccg ttgtgcgttg aataacccca cagcagaacg ctgtggggtt
      241 gtcacatcag agataggtaa tctggaatgt ggtcagggtt tcgaactggc cgagatccgg
      301 cgcatcggtt tcacccacca gccaggcttt aaagttcagc gtttgcttgt ctttaccctt
      361 gctagttgaa gcatccatca cgattggctg aacaatcttt tcgcccactt tataaccttt
      421 gccatgggta tcgccgatta ccaggctcac gtggttcatg gcaacaccgc tgtcaacatt
      481 gaataacgca tagttatctg cgttggctgg ccccgcgtag ttagtgccgg tgaacgtggt
      541 ctccatgttt gattcagtgg taaacacgca atcctgtaaa tggatctgga aatctttcgg
      601 tgtggtagtg ccgccgccag tcagtttagc cgcaccgata tcgcccatat cgacttccag
      661 tttatgatcg tctggaacga tggagcacgg ggagtcttca atagtgccgt aaaaacgtgc
      721 ggtaccgttg ttacctgcgg caaaagcaga actggagacg actgcgccca tgaccattgc
      781 ggcagcaatg gctgttttgg caaacttact cattatttgt c
//
LOCUS       DAJOTB010000372          817 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJOTB010000372 DAJOTB010000000
VERSION     DAJOTB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.384"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..304
                     /locus_tag="OHN28_005034"
     CDS             <1..304
                     /locus_tag="OHN28_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300264.1"
                     /translation="ENSPTVNYLANKLNRENLVFPDDFKLSVTFKSLTSREGNECLHN
                     RYVLSNVAGVCFMHGLDEGEGTDDVSILSKEGYNKRWEHYTTNNVFDLIEEREVIC"
     gene            complement(527..756)
                     /locus_tag="OHN28_005035"
                     /pseudo
     CDS             complement(527..756)
                     /locus_tag="OHN28_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          239 a          172 c          138 g          268 t
ORIGIN      
        1 agaaaactcc ccaacggtga attatttggc taataagttg aaccgcgaaa accttgtctt
       61 tccagacgat tttaaattat cagtaacatt taagtcttta acttctcgcg agggtaatga
      121 atgtctgcat aatagatatg ttctttctaa tgttgctgga gtttgtttca tgcatggttt
      181 agatgaggga gaaggaacgg atgatgtctc aattttgtcg aaagagggat ataataaaag
      241 atgggaacat tacactacga ataatgtttt tgatttaata gaagagcgtg aagttatatg
      301 ttaagtattt cccttactgt tttcaattaa atcctacaaa cttattagga ccctttaaaa
      361 tttattcaac acgccaataa aaaatctaat tctattaatc ttgacctggt ctatcaaaat
      421 gatagaccag gcttctgtta tctacattac ttctagtgaa atagccaacc aatcgtccct
      481 ataaggcagg ttaaatagtt ttaatttagc aaatttgata aacaactcac tcgccattac
      541 cccgattgat cttcgcatag tcaaacggcg taatcgctct ctcccgattg gcatccagaa
      601 aatctgccca ccactgcagc atcagcttgc gctcatccag atgctccgct ttatggatat
      661 acgctgcctg cactgagtta cgtccatgtg gctcatctga cgttcagccg ctttatctcg
      721 tgctgttctg gtctcaatac ttccatgccg ttgcaattac ctcttaaacc attccttatc
      781 attttcatgt cttcgataag aaatcttcat cattcat
//
LOCUS       DAJOTB010000373          799 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJOTB010000373 DAJOTB010000000
VERSION     DAJOTB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.385"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..614
                     /gene="ydeO"
                     /locus_tag="OHN28_005036"
     CDS             <1..614
                     /gene="ydeO"
                     /locus_tag="OHN28_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCQ2300265.1"
                     /translation="IGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIA
                     YLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWK
                     LKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYA
                     STSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTDNTMNALAS"
BASE COUNT          247 a          160 c          157 g          235 t
ORIGIN      
        1 aaatcggatt taatgaaaat accattaaaa aatatctaca atgcactaac atccagacag
       61 tgacgatgcc tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact ggattgctta
      121 atgaaatgat tgcttatctc aactcggaag aacgcaatca tcataatttt tcagagcttt
      181 tgcttttttc ttgcctgtct atttttgccg catgcaaagg tttcattaca ctattaacta
      241 acggtgtgct atccgtttcc gggaaagtaa gaaatattgt caacatgaag ctggcgcacc
      301 catggaagct gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa
      361 aactaaagca agagcaaacg acattctcac agattctttt agatgccaga atgcagcacg
      421 cgaaaaattt gatacgcgta gaaggttcag tcaataaaat tgctgaacaa tgcggttatg
      481 ccagtacatc ttattttatt tatgcgtttc gcaaacattt cggcaacagt ccgaagagag
      541 tttctaagga gtaccgttgt caaaggcaca cgggtatgaa cacggacaac acgatgaatg
      601 ctttagctag ttgattattt gctaacgagt agtcaaccac acacgctgcg taagaattaa
      661 atggggcagc cattccctgc cccgcgttgt ttccaggcga tatatttctt gaaataaata
      721 agtgacagcc atcacatatt tatgcatttt cataacctgt tgcatgatta tttatgatct
      781 cagctcggca atttgtcag
//
LOCUS       DAJOTB010000374          786 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJOTB010000374 DAJOTB010000000
VERSION     DAJOTB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.386"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            207..>786
                     /locus_tag="OHN28_005037"
     CDS             207..>786
                     /locus_tag="OHN28_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2300266.1"
                     /translation="MSKKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKS
                     ATYDFKASYVRADASLAATAGYVKTNTAYTVTY"
BASE COUNT          235 a          149 c          158 g          244 t
ORIGIN      
        1 tgtatattaa cttttgctta aaaatgaatt aagtggatat ttaaatttaa atgtgtacat
       61 tattctactt agtgatgaac agatgttaaa ttcagtaaat cttcacattc atcattaaca
      121 aactaatctc ttttcctcta tcgggaatcc ctgcctgaaa gggcccaatt gtagttaaac
      181 tcatggaata tgattcaagg atttttatgt ctaaaaaatt aggttttgcg ctctctggtc
      241 ttatgctggc aatggttgca ggtagtgctt ctgcagatat ggacggtggt cagttaaata
      301 tcagtggtct ggttgttgat aatacctgtg aaacccgcgt agacggcggt aataaagatg
      361 gcctgatcct gctgcaaact gcaaccgttg gagaaatctc tgagggtgtg ctggacacta
      421 ccgttggtgc taaggctaaa ccattcagca ttactgttga ctgtagtaaa gctaatccgg
      481 caccaggcag cacagcaaaa atgacttttg gttcagtctt ctttggtaac agtaaaggaa
      541 ccctgaataa tgacatgtca attaatactc catctgacgg tgttaatatt gctctgcaca
      601 atattgaagg ttctactatt aagcaggtta aaatcaacaa tcctggcgat atctattcca
      661 aagcactgga tgcaacttcc aaatccgcaa cttatgattt taaagcgtct tatgttcgtg
      721 cagatgcaag tctggcagcc acagcaggtt atgtaaaaac taacactgca tataccgtta
      781 cttatc
//
LOCUS       DAJOTB010000375          778 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJOTB010000375 DAJOTB010000000
VERSION     DAJOTB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.387"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          232 a          154 c          171 g          221 t
ORIGIN      
        1 gttcgtactc attttggagt tagctcgcat tcgtaatgcg aagggcgtag gttctactcc
       61 tctcagcaag gatactgccg gcagaatcct tcatgaggct ggaatgctca aaaagtccac
      121 cagcgtggag ggatggctga tacgtatgcc acggctgaga cacctcaagc atgcacgtcc
      181 ctgggcttat acgctgttgt ttactccggt atctgaaacg gaaaccggag aagaataaaa
      241 aaacacgtaa atatgaaaac tgttagccca catagcccaa caaaaatatt tgatagttaa
      301 ccttctgtta ctaaagaaaa caggaaagta aaagtgggct aaagcttatg cgccctcgat
      361 gttgggctag ccccaaaaac ggtaaattta gcttaagtgc ataattggtt agctcaaaag
      421 cattattttt catttaaata aattagttaa ttggtcttgt ttagatgatt caactgggct
      481 gactactttc tttgtatata ctccggataa attttcccag ctaacttgcc taatcatcac
      541 tctgatgcca gaaatgaaca gaacgcaaac catctataac ttattgagga ttttgaaaaa
      601 aattgattgg gggcttgagt tatatgatga ctatgctaat ttaatacggc acatgcaggt
      661 agatttgttg gttgtggtat cgcaatcagt gttaacaagg tcgggagtat tcgccctctg
      721 actgccgtca agtcatcttg gcgtcaccgt taaatgcgta agagtacctg catgtgca
//
LOCUS       DAJOTB010000376          775 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJOTB010000376 DAJOTB010000000
VERSION     DAJOTB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.388"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            11..>775
                     /locus_tag="OHN28_005038"
     CDS             11..>775
                     /locus_tag="OHN28_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300267.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYETIMENNTNKESLCLQSEKGGKTSYKDITK
                     IQLATTEKQKLLASLEVVNSMSSYVELLTVGMDVEDSKQIILLNQALDHANNAQKLLG
                     LSEGEELTKSVVAVKDLVQYFNGLYKEKIKSENVGKTVGSDWPRLQRNLQDLLDDIAT
                     KQQQMDRNNYANISNNFYYYNKAQANTKNIEFKTLKS"
BASE COUNT          276 a          116 c          164 g          219 t
ORIGIN      
        1 gaggtgggtt atgattagat atagtggaat atttctatcg ctgttaatgc tttcaggttg
       61 tgcaaccgaa gcattgaaga atgaacaagc tgaaactgtt attactgcgg gtaacaaggc
      121 ggttgcggcg aatgcaagtt tctttaaaca atactatgct tctcgtgaaa actttcttgt
      181 taatttttat gcgactaatc cggattgtgc attgcgacct gattacgaaa cgattatgga
      241 aaataatact aataaagagt ccttatgttt gcaatcagag aaagggggca aaacatcgta
      301 taaagacatt actaaaattc aattggcaac aacagaaaaa caaaaactac tggcttcact
      361 tgaggtcgtt aacagtatga gttcctatgt ggagttgttg acggtgggta tggatgttga
      421 ggatagtaaa caaattatat tacttaatca ggcgctcgat cacgcaaaca acgcccaaaa
      481 actacttggc ctttcagaag gtgaagaatt gactaagtca gttgtggctg ttaaagattt
      541 agttcaatat ttcaatggat tatacaaaga gaagattaaa tcagaaaatg ttggtaaaac
      601 ggtaggtagt gactggcctc gacttcaaag aaatttacaa gatttactcg atgatatagc
      661 aacgaaacag cagcaaatgg atcggaataa ttatgcgaac atcagtaata atttttatta
      721 ctacaataag gcccaggcaa atactaaaaa tatagagttt aagacattaa agagc
//
LOCUS       DAJOTB010000377          766 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJOTB010000377 DAJOTB010000000
VERSION     DAJOTB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.389"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..444)
                     /gene="fimE"
                     /locus_tag="OHN28_005039"
     CDS             complement(<1..444)
                     /gene="fimE"
                     /locus_tag="OHN28_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCQ2300268.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAE"
BASE COUNT          254 a          166 c          143 g          203 t
ORIGIN      
        1 ctcagccagt tcataaccac aagcatgcct taacatatga ggatgagtct gcgttacggt
       61 tccagcttca ataccggcat cgcgaataat gcgataggcc tgctggcgag aaagccgact
      121 cccacggcga gaaataaata tagcgtcggt ccggtcagcg cctttccagt tagcacgttc
      181 ctgggtccag cgttccacgg cttcacgctc atcaaaacgt aacgggtgaa cggtagaaaa
      241 tccgttcttc agtcggcgaa tatttattcg accttcatta aggtcaagat cctgataatg
      301 cagatcaagc agttcactaa tacgcatccc atgccgatat gccaacagaa taagacaata
      361 atctctggct cccgttgccc cgtaacacac cgcctgcatc atggcctgaa cttctttacc
      421 ggtaagataa cgacgtttac tcacaatagt agtactcctg actgagatat atttgaatga
      481 atacctatag gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag ataaccacac
      541 ccgaagtgat gtggctatca gtcaattaca ttaaatatca tacaaaaata aaatatcact
      601 gatgtgacaa catctatttt cattgatttg attcaatata acaatagatt gcagtggatt
      661 aacttccagt tgcttaaaaa ataaagaaaa aagtatgaaa acaggcgaat ttaagtgatg
      721 tgtaaattta tgaatgattt tgtttattta ttttgaagaa cataaa
//
LOCUS       DAJOTB010000378          763 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJOTB010000378 DAJOTB010000000
VERSION     DAJOTB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.390"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            23..619
                     /locus_tag="OHN28_005040"
     CDS             23..619
                     /locus_tag="OHN28_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCQ2300269.1"
                     /translation="MNNTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
BASE COUNT          204 a          152 c          174 g          233 t
ORIGIN      
        1 atattaatca gggagaataa aaatgaataa cacggatctt atccatctca ttaaacattt
       61 tatgcgtaac gaacataaag cagttgaaga agttattgat tcccctcttt cggaggtcgc
      121 aaatttaatt aaagtattac aatcttgtca gggaaaagtt gtttttattg gtgttggtaa
      181 gtccggtatt attgccagga aactcgccgc aacatttgcc agcaccggaa ctccctcgtt
      241 ttttgttcac ggtacggaag ctgtacacgg cgaccttgga atggtggcga aagacgatgt
      301 tgttattctt atttcaaaca gtggtgagac ggcggaaatc ctggcgacac tgcctagtct
      361 gaaaaaaatg ggtaattatt tgatctcttt tacccgcagt caccactcat cgcttgcgat
      421 aagttgtgat ttatctgttg aaatccccgt caaaagtgag gcggataatt taggtttagc
      481 accgtcatgc tcgtcgactg ttgttctggt tgttggtgat gcggtggcgc ttgcactctc
      541 tgaattgaaa aaatttactc gtgctgattt cggcttatat catccaggag gtgcactcgg
      601 cattaaagca aattcataat ttcaaaatat aaatacagta accggtcacg ccatcttttg
      661 gggtggcgtt ctttgaagtg agtgtccgct atgaaaaatg ttctggtcac cgtccccagc
      721 ttttcagcac gctgtgtttc cgccagcaag ttgctaagag aga
//
LOCUS       DAJOTB010000379          749 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJOTB010000379 DAJOTB010000000
VERSION     DAJOTB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.391"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..168
                     /locus_tag="OHN28_005041"
     CDS             <1..168
                     /locus_tag="OHN28_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCQ2300270.1"
                     /translation="PTQQETLGEIVTEILKDGRNLSRKSLCAKLLCRLEQATGEEEQK
                     HYNALIGLLFE"
     gene            211..483
                     /locus_tag="OHN28_005042"
     CDS             211..483
                     /locus_tag="OHN28_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCQ2300271.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKNMFTGKKINRIH"
     gene            512..>749
                     /gene="ariR"
                     /locus_tag="OHN28_005043"
     CDS             512..>749
                     /gene="ariR"
                     /locus_tag="OHN28_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCQ2300272.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEI"
BASE COUNT          263 a          117 c          159 g          210 t
ORIGIN      
        1 cctactcagc aggaaactct cggggaaatt gtgactgaga ttttgaaaga tggacgtaat
       61 ctgagtcgaa agtcgctttg tgccaaactt ctttgccgac tggaacaagc gacaggggaa
      121 gaggaacaga aacattataa tgcactaatt gggttgcttt ttgaataatg caacagcctg
      181 attatttaac aggttagcta tgaagtcgtt atgaagacat ctgataatga aagtattaaa
      241 tatgaaatta ctggccaggc ggtgctccag atactgcgca tgaagataaa tttttcgttg
      301 cagactctta ttaagcaatt actcgtaatg aaatcggctg aggaaaatac tttccgacga
      361 gatttaattg acagcataat tcgtgatttt agcaacagtg atacaggagg gccaaaccga
      421 agaacagcga tcgctgacaa taaaaatatg ttcactggta agaaaataaa cagaatacat
      481 taaaatttca taagtaagat gagaggttac catgcttgaa gatactacta ttcataatgc
      541 aatatctgat aaagcgttat caagttactt tcgtagttca ggtaatttgt tagaagaaga
      601 gtcagctgtg ttagggcagg ctgtgaccaa tttaatgctt tcaggtgata atgttaataa
      661 taaaaatatt atcttaagtc tgatacactc cctggaaaca acaagtgata ttctcaaagc
      721 tgatgtgatt agaaaaacac tggaaatcg
//
LOCUS       DAJOTB010000380          716 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJOTB010000380 DAJOTB010000000
VERSION     DAJOTB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.393"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            78..275
                     /locus_tag="OHN28_005044"
     CDS             78..275
                     /locus_tag="OHN28_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat protein"
                     /protein_id="HCQ2300273.1"
                     /translation="MSTNVVLNGDVSVGEETFVGSCTVVNGQLKLGSKSIIGSGSVVI
                     RNIPSNVVVAGTPTRLIRGNE"
     gene            272..>716
                     /locus_tag="OHN28_005045"
     CDS             272..>716
                     /locus_tag="OHN28_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HCQ2300274.1"
                     /translation="MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKAD
                     KLISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDDYLHLMEYAVSLNLDVFSTPFDE
                     DSIDFLASLKQKIWKIPSGELLNLPYLEKIAKLSIPDKKIIISTGM"
BASE COUNT          252 a           94 c          146 g          224 t
ORIGIN      
        1 ctagaataca tgatgccgtt gtaataaata ctaggagttt aattgaacat ggtaatgaaa
       61 taggctgctg tagcaatatc tctactaatg ttgtacttaa tggtgatgtt tctgttggag
      121 aagaaacttt tgttggtagc tgtactgttg taaatggcca gttgaagcta ggctcaaaga
      181 gtattattgg ttctgggtcg gttgtaatta gaaatatacc aagtaatgtt gtagttgctg
      241 ggactccaac aagattaatt agggggaatg aatgagtaat atatatatcg ttgctgaaat
      301 tggttgcaac cataatggta gtgttgatat tgcaagagaa atgatattaa aagccaaaga
      361 ggccggtgtt aatgcagtaa aattccaaac atttaaagct gataaattaa tttcagctat
      421 tgcacctaag gcagagtatc aaataaaaaa cacaggagaa ttagaatctc agttagaaat
      481 gacaaaaaag cttgaaatga agtatgacga ttatctccat ctaatggaat atgcagtcag
      541 tttaaattta gatgtttttt ctaccccttt tgacgaagac tctattgatt ttttagcatc
      601 tttgaaacaa aaaatatgga aaatcccttc aggtgagtta ttgaatttac cgtatcttga
      661 aaaaatagcc aagctttcga tccctgataa gaaaataatc atatcaacag gaatgg
//
LOCUS       DAJOTB010000381          714 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJOTB010000381 DAJOTB010000000
VERSION     DAJOTB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.394"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..628)
                     /locus_tag="OHN28_005046"
     CDS             complement(<1..628)
                     /locus_tag="OHN28_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300275.1"
                     /translation="MKSDLTIKNRYCTIPQAKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     LLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFQISATYL
                     RDLVLFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFICLINNLPAR"
BASE COUNT          223 a          147 c          106 g          238 t
ORIGIN      
        1 cacgcgcagg caaattatta attaaacaaa taaaaaaacc caaatctcgt tcaacaccac
       61 gattatatct aataccagcc ataatatact gctcattagt catatacagt gttgccgaag
      121 agggataatc aaaaaggaca agatctctta aataagtagc agaaatttga aaggtaaaat
      181 catcctccat taggcatgtc gctaactcta actgatttct ggtagttaat tctgaagggt
      241 ctagacctag agtaatcgct gcggttcgta tttgtatacc cattgagcca aatgatgttt
      301 tattttcagg catatctaac aatccgatca ttctttttgt ttcatatatc tgaaacttat
      361 aattttcagg catacctcct gactcaatcc aggccacgcc gcccagtaac tctggacgaa
      421 ttccagcggc gtatgcatgt ttaattattt tgtctctatt atattgtaca tatgcatctt
      481 tataagcatt taaataatat actcctgaac ggcgacgcga atcatttttt cctaacttcc
      541 agagtaaaac gtccatttca tcccatttac ggaacttcgc ttgtggaatt gtacaatatc
      601 tattttttat agtgagatcg cttttcatat ttaaaaaatc ctttcttccc aacaactata
      661 tgattccgta ctcgcgatat ggtgcttcca tgttatattt ttataattaa gaac
//
LOCUS       DAJOTB010000382          713 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJOTB010000382 DAJOTB010000000
VERSION     DAJOTB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.395"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          216 a          136 c          150 g          211 t
ORIGIN      
        1 gaggaggcga gcacaatatt ttatcccatg tctgcacgat gccagtttga acaggatgcc
       61 tgtgaactgt atgcatggat tatgacccga ataaggcaga ataattggcg tgctatcagg
      121 aaaacagaca ttgaaagata tggtcccaat cgactgagaa gagcagaaaa acttacacct
      181 gtactcaatc agttaatctg tcaggggtat ttatgtatca tcagaatgcg atcgcatcag
      241 gcactttatg tttctgttta ttgtaacaat ggttacctcc ttcctttagg cgcaatgtat
      301 tacgaacagt ttgatattgt tctacccgag aatattcata aagcgaaagc atatcacgtt
      361 aatattccac cggaattaat tccgtcattt gcgtcacctt tttcagagta tagcttattt
      421 tgaaataacc ttacacagat aaaacaaaat caaccaacct tcattcggag gttggtgata
      481 aggcgttttg catatcctgc acctatgcaa atgttcacca attatggggg gtgaatgaga
      541 atttctctga aaaaaccgct acggtagcaa cagtagcagc acatgcacca catctgtgat
      601 ttaattttgt tttcataatg acctgctgtc agagctggtt gaatacagga gtgtgcgcac
      661 tggtggaagc atggctttcg ttttttattg atcgaaagtg aatcgaaaga tta
//
LOCUS       DAJOTB010000383          695 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJOTB010000383 DAJOTB010000000
VERSION     DAJOTB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.396"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(15..464)
                     /locus_tag="OHN28_005047"
     CDS             complement(15..464)
                     /locus_tag="OHN28_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300276.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPLLLNYFYSQDLVLNISLFIFSEVTKVTTIIWCKRQGKV"
BASE COUNT          261 a          137 c          108 g          189 t
ORIGIN      
        1 tgttgctatt tctgtcacac tttcccctgt cgtttacacc agataatcgt tgtcactttt
       61 gtgacctctg agaagataaa caacgaaata tttaagacca gatcctgtga ataaaaataa
      121 ttaagtaaca acggtattac ggagattgaa cagcttacgg tagcaaaacg cataaactcc
      181 tgaatcgcca aaaaagagaa actaaataat ttttgtctta aaaaacattg cgcagaaaag
      241 aataaagaga acaataatac tatttgtagc gtaacagtgg cgactgcata agcgtaaaaa
      301 ccgccttcaa caccgatatc cttaagtaaa gaaagtgaat gaacgatatc ggaataaaaa
      361 gggatacgac cattaccaag cgagtagaac agatatgtgg ctatcgcgat aacatccata
      421 acaccccaaa aaagaagaat gtatttttta tattgacgtt gcataaagta aatccttttt
      481 acgaaaacaa atattaccag gaattattat aattgcgggc gttttggatt aacactccac
      541 ccgtattttt taaacctctg tcagctatac cttcactaaa taagacatcg atacgccctt
      601 tacttttatc gtgatacaga atactgcatg agatacacct tataccctct ttggatttac
      661 gaaaatctaa ccaataacga ccatcataaa atacc
//
LOCUS       DAJOTB010000384          693 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJOTB010000384 DAJOTB010000000
VERSION     DAJOTB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.397"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            20..340
                     /locus_tag="OHN28_005048"
                     /pseudo
     CDS             20..340
                     /locus_tag="OHN28_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            complement(356..>693)
                     /locus_tag="OHN28_005049"
     CDS             complement(356..>693)
                     /locus_tag="OHN28_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCQ2300277.1"
                     /translation="RWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMT
                     DGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIG
                     HYLNIKHYQ"
BASE COUNT          183 a          164 c          164 g          182 t
ORIGIN      
        1 attctttaag gtcgttttta tgatgaaaaa tccattagct tattttcata ttgccacctt
       61 tttattgtca ggtaaaagtc gtaatctgtc agaatactgg aaagccgaag tcatcatgcg
      121 ggataagttt gtcttacacc gcctgataag aaacgggttg cagagacaac gaggctttct
      181 gatgtggtgg cgcctggcca atgagatgtt tatctctgga aaccaaaaac aaaggaaatg
      241 tgcgataaag ataaaaaatg cacttatgga acggtatgga tgtgatatcg gcctgggcgc
      301 gcggattggt aaaggcctca tgttacctca tcatgcagga ggtaatgact ccaacttatt
      361 gatagtgttt tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg
      421 aaaacgacag cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc
      481 gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt
      541 catacagcgg ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca
      601 taagacgccc cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc
      661 ggagactgtc atacgcgtaa aacagccagc gct
//
LOCUS       DAJOTB010000385          693 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJOTB010000385 DAJOTB010000000
VERSION     DAJOTB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.398"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..604)
                     /gene="upp"
                     /locus_tag="OHN28_005050"
     CDS             complement(<1..604)
                     /gene="upp"
                     /locus_tag="OHN28_005050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCQ2300278.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAG"
BASE COUNT          141 a          197 c          168 g          187 t
ORIGIN      
        1 caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc tggtcaatcg
       61 atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata ccttctggtg
      121 cagctaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg
      181 tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc atacgctcat
      241 cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct tcattacggt
      301 acataccgac gacgctgatg cgcgcgctcg gaacgttttc cagcacaccg tccatcatcc
      361 ccagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc tggtcgattt
      421 ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt
      481 cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg gtgctgatat
      541 cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact tccacgatct
      601 tcatactctt tctccttgga ggggcagcca caaaaaaaat cgacggatta tacctccttt
      661 cttcaaggcg gcaatattat tttcgttgac ttt
//
LOCUS       DAJOTB010000386          690 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJOTB010000386 DAJOTB010000000
VERSION     DAJOTB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.399"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(41..>690)
                     /locus_tag="OHN28_005051"
     CDS             complement(41..>690)
                     /locus_tag="OHN28_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2300279.1"
                     /translation="SVRFLFMHRGKRVHSSYINNSLIPLLCRKAGIPEQDARGKITSH
                     RARATIASQLYNAREPLDIFELQKWLGHSSPESTRHYVEITPTRLAGSLDKAGYFERN
                     RRMVSVLIDQDAIAEGLVASGKPWRYYDLGHGFCTYDFFEQCPHRMACAKCSFYMPKS
                     SSEAQYLEGRQNLLKLMQEIPLTDDEQAAVENDIQAVDKLLNKLTGGAPPGNKRK"
BASE COUNT          178 a          159 c          147 g          206 t
ORIGIN      
        1 tgtatttaaa ttaaaatgaa attagtgctg agctatttta ttactttctc ttatttcccg
       61 ggggcgcgcc cccagtgagc ttattcaaaa gtttgtccac tgcctgtata tcgttttcta
      121 cagcggcttg ttcgtcatca gtcaaaggga tttcctgcat cagcttgagt agattctggc
      181 gaccttccaa gtattgtgct tcagatgaag attttggcat ataaaatgag cattttgcac
      241 atgccatccg atgggggcat tgctcaaaaa agtcataagt acagaaaccg tgtccaagat
      301 cataataacg ccatggcttc ccggatgcta ccagcccttc agctatcgcg tcttgatcaa
      361 tgagaactga aaccatgcgg cgattacgtt caaagtaacc agctttatcc agtgatcctg
      421 ccaacctcgt cggtgtaatt tcgacataat gtcgggtaga ttcaggtgaa gaatgcccca
      481 accatttctg tagctcaaaa atatcaagtg gttccctggc gttgtaaagc tgactggcta
      541 tagtggctct tgcccgatgg ctggtgattt tacctctggc atcctgctca ggtatacctg
      601 cctttctgca tagcaaaggg attagcgaat tattaatata actactgtgt acccgtttcc
      661 ccctgtgcat gaacaaaaaa cggacgcttt
//
LOCUS       DAJOTB010000387          687 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJOTB010000387 DAJOTB010000000
VERSION     DAJOTB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.400"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..270
                     /locus_tag="OHN28_005052"
     CDS             <1..270
                     /locus_tag="OHN28_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCQ2300280.1"
                     /translation="LRRAEKLTPVLNQLIAQNYFRIIKMRSHQALYVSALDNNGYILP
                     FGAMSYEPFDIVPPQYNHNAKTYSVVIPPALIQSFTPDSSAYTLF"
BASE COUNT          202 a          126 c          131 g          228 t
ORIGIN      
        1 ctgagaagag cagaaaaact tacacctgta ctcaatcagt taatcgctca aaattatttc
       61 cgtatcatca agatgcgatc gcatcaggca ctttatgttt ctgctcttga taataatggt
      121 tacatccttc ctttcggcgc aatgtcttac gaaccgtttg atattgttcc accccagtat
      181 aaccataatg cgaaaacata ttccgttgtt attccaccgg cattaattca gtcatttaca
      241 cctgattcct cagcttacac cttattttaa aacaattttg tgagtagaaa acgaaaatca
      301 taatccttcg aatgaaggtt gatgataagg tgtgttgcat atcctgcacc tgtgcaaata
      361 ttcaccaatt attgggtgtg aatgaaaatt tctctgaaaa aatcgctatg gtagcaacag
      421 tagcagcaca tacactacat ctgtgatttg gttttgtttt cataatgacc tgctgtcaga
      481 gctgattgaa tgctgggatg tgcgcactgg tggaagagtg gttttcgttt cagatataac
      541 gaaaggcaat cgaaagattg ttttaaacat ggattaaagc taatcattaa ccatattgtg
      601 tgagttttta tatataagtt tgtttgattc ttgccgtgat gagtgctggg gtatatgacg
      661 atgtcgctct ctttctgaat aacaaat
//
LOCUS       DAJOTB010000388          684 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJOTB010000388 DAJOTB010000000
VERSION     DAJOTB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.401"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..496
                     /locus_tag="OHN28_005053"
     CDS             <1..496
                     /locus_tag="OHN28_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300281.1"
                     /translation="IKTSPQLLGLKDNVYFCKIDSSQMLFPNQVGVGLTQIAPLIIAA
                     NIVQDGLIAIEQPELHIHPALQLAVGDLFTQYPLDVKRPMFLVETHSEHILLRILKRI
                     RQTTDNELPESNYPVKPDFISVIVFEDNNGSTVTRKIDITDDGDFKQKWPKGFFEERR
                     GELF"
BASE COUNT          231 a          120 c          124 g          209 t
ORIGIN      
        1 tataaaaacc tcccctcaac ttttaggtct gaaagataat gtttacttct gtaaaataga
       61 ttcaagccaa atgttatttc caaatcaagt cggtgtcgga ttaactcaga tagcacctct
      121 tattattgca gccaatatag ttcaggatgg attaattgct atagagcagc ctgagctaca
      181 tattcatccc gctttacaac tcgctgtagg tgatctgttt acccaatatc cccttgatgt
      241 taaaagacct atgtttttag tcgaaacaca tagtgagcac attttgcttc gaattttaaa
      301 aagaattaga caaacaacgg acaatgaatt acctgaatca aattacccag taaaacctga
      361 ttttatatca gtgatcgttt tcgaggataa taatggttcg actgttacta ggaaaataga
      421 tattactgat gatggtgact tcaaacaaaa atggcccaaa ggtttcttcg aagaaagaag
      481 gggagagctt ttttaatgtt ctacgaatgc gccatttcac cagaggcttt gtttgagatc
      541 gcaattgatc ggagaaatta tcgtgacttc ataaaaggat tttctacggg cggtaacttt
      601 ctctatagtg aattaccaaa gctgaagaga aataaaaaac aattgcttgg gctattaaat
      661 gctaatcata gtgaacttca aaaa
//
LOCUS       DAJOTB010000389          677 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJOTB010000389 DAJOTB010000000
VERSION     DAJOTB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.402"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            28..>677
                     /locus_tag="OHN28_005054"
     CDS             28..>677
                     /locus_tag="OHN28_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HCQ2300282.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANTALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQE"
BASE COUNT          208 a          158 c          167 g          144 t
ORIGIN      
        1 ttacgtcata aagagaggaa ttctgaaatg ggtaaaatct tgcttgtcgt atcagataag
       61 ggtggtgtag gcaaaagcac ctacgtggct aacacaggct caatgctggt caataaaggt
      121 aagtcggtaa ttatcctcaa gacagataaa aaccacgatc tgttgagctg gaacgaaaag
      181 cggacagata acggcttact cactatcccg gttcacgcag cttacggcaa cgtcagcaat
      241 gaaattaagc gacttagtaa gctgtgtgaa gtcctcattg tcgattgccc cggccacgac
      301 agccaggagt tccgtagcgc cctgacagta tcagatatcg tggtaacact ggttaagccg
      361 tcctctgatt tcgaaagcga aacactgacc agtgttacag agaaaatccg caccgctcag
      421 aaagctaaca ctgccctgga accgtgggtg ctgttcacca gaatcaactc agcaaaaccc
      481 cgccacagaa aagcggccat tgatctggat aaattactgc ggtcagacaa catctggatt
      541 caacctctta aaacccggat atccgagctg gatgtgtatg aaagcgcctg taatgaaggg
      601 gcaggggtac atgacgttag ccgggcatca agcctttcaa cagcgaaagc acagattgaa
      661 ttagtggcgc aggaaat
//
LOCUS       DAJOTB010000390          656 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJOTB010000390 DAJOTB010000000
VERSION     DAJOTB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.403"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          213 a          140 c           91 g          212 t
ORIGIN      
        1 acattctcaa catcttcaga cttccataca tactgggtgt taatttttca cttttacagc
       61 cagcatggag ttttctgtcc taaaaacaga acagatttac tctggaatta ttttctctac
      121 ctcagaaaac atggtctcag gaatctgcta tggcttcgta gtttctgttg ttcttttcat
      181 aaaagttata agtcattgtc ataatcatac gataatcact ttatgttagc ttttactgtc
      241 acacgctact gactttttca accattgcca gaaaggggct ctttttactt ccatttcacc
      301 actcaacaaa tgcaaaactt gtccagtaaa gcggaccacc tcttgctcac aagcaacgaa
      361 tcgtctccag gaacagtcct caaacattgc agaaacaaaa acaacaaaac cctcattatc
      421 actgatgttc ggaaaatttc cctgaacaaa aaaacgaaaa caaaatgaga cgcattatca
      481 taaatgtagt caaaaagacg ttatggtggc tttcagttaa atggtactat ttctcataaa
      541 tccacttaac tttttttact gattcatccc agagaaccac tttatcagta aaaaatgaat
      601 tatatagatt gacgagtttt tctgtttcag ttattcccaa tatagtatgt atcagt
//
LOCUS       DAJOTB010000391          655 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJOTB010000391 DAJOTB010000000
VERSION     DAJOTB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.404"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>655
                     /locus_tag="OHN28_005055"
     CDS             <1..>655
                     /locus_tag="OHN28_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCQ2300283.1"
                     /translation="VRGYFIANQNFKLHPNVATIIAQIACLDGSLPQGSPCSPVITNL
                     ICRILDFRLSKLAVTYGCSYSRYADDITFSTNKKNIPDALVSNEKENEPGKILVEEIH
                     RAGFTLNHNKNRVSRCTSRQQVTGLTVNKKINVSREYIKNTRAMAHSLYFEGSYTLIE
                     KDGKHRKGTLSELEGRFAFIDMLDKYNNVEAKKNARPERYVVKGFGLDFKQRLNSREK
                     "
BASE COUNT          238 a          101 c          136 g          180 t
ORIGIN      
        1 cgtgcgagga tattttattg caaaccaaaa tttcaagtta catccaaatg ttgcaaccat
       61 tattgcgcag atagcctgcc tggatggatc gcttccgcaa ggaagccctt gttctccagt
      121 aataactaat cttatttgta ggattttaga tttcagatta tcaaagctag cagtcacata
      181 tggttgtagt tacagccgct atgcagatga cattacgttt tcaacaaaca aaaaaaacat
      241 ccctgatgca ttagtttcta atgagaaaga aaacgaacca ggtaagatat tggtagaaga
      301 aattcatcgt gcaggcttca ctttaaacca taataaaaac agagtgtcta ggtgtacatc
      361 aagacagcaa gttacaggtt taactgtaaa taaaaaaata aatgtaagca gagagtatat
      421 aaagaataca agagcgatgg cgcattcttt atactttgaa ggttcgtata cacttattga
      481 gaaagatgga aaacatagaa agggcaccct tagtgaatta gaagggcgat ttgcatttat
      541 cgatatgctt gataaatata ataatgtgga agcaaagaaa aatgcgcgtc ctgagagata
      601 tgtggttaaa ggatttgggt tggattttaa gcagagactt aactccagag agaaa
//
LOCUS       DAJOTB010000392          628 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJOTB010000392 DAJOTB010000000
VERSION     DAJOTB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.405"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..494
                     /locus_tag="OHN28_005056"
     CDS             <1..494
                     /locus_tag="OHN28_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HCQ2300284.1"
                     /translation="KFEKTRRNEIQRFIRNGGEIKCISQLSDKEISSSYISLFHSRFG
                     GTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAIDIVLKSESSCNVYYDVPNGAVL
                     NDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIYSIGAFRPEWKYKLLWSVPCKVGK
                     CLC"
BASE COUNT          210 a           92 c          121 g          205 t
ORIGIN      
        1 ctaaatttga aaaaactcgc cgaaatgaaa tccagagatt tatcaggaat ggcggagaga
       61 ttaagtgtat aagccagtta tccgataaag aaatatccag ctcttatata tcactttttc
      121 attcccggtt tggaggtacc ctaccttgct acgaatatga taatctgtta atgtttattt
      181 ctcacttacg agaacttatg tttggccatg tgctattctg ggataataaa ccgtgtgcta
      241 ttgatatagt actgaaatct gaaagctcat gtaatgttta ttatgacgtt ccaaatggtg
      301 cagttctgaa tgatgagaat tgcatgaaac taagtccggg aagtgtcctt atgtggctga
      361 atatagataa agcgcgaagg tattgtcagg ataataataa aaagatgata tattctatcg
      421 gtgcattcag acctgaatgg aaatataaac tactatggtc tgttccctgt aaggttggta
      481 aatgcttatg ttagaatatg tataaaggta agtaactata taagagcgtc cagtctgcaa
      541 atactcctct aaacatggag gagtagattt ataatatcat aacatttact aaatcgtttt
      601 acattaattt caaatttgaa attaatgt
//
LOCUS       DAJOTB010000393          613 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJOTB010000393 DAJOTB010000000
VERSION     DAJOTB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.406"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..602
                     /locus_tag="OHN28_005057"
     CDS             <1..602
                     /locus_tag="OHN28_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
                     /protein_id="HCQ2300285.1"
                     /translation="QQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPCEKWVT
                     DITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEKPLL
                     HSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRNV
                     EELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT          186 a          129 c          172 g          126 t
ORIGIN      
        1 tgcaacagct cggcctgaag tctcctgtgc gcctgaagaa ataccggtct taccggggaa
       61 atatgggact cgctgcagaa aatatccttc agcgacagtt caaagcggaa gctccctgtg
      121 agaaatgggt gacagatatc accgaattca gggctggcgg acaaaaactg tacctgtccc
      181 cgatacttga tttgtttaac ggggaaatcg tggcgtggga aacggcctgc aggcctacag
      241 aggagctggt gaagcggatg ttaaataagg gtctggaaag ccttgctgaa ggggagaaac
      301 cgctgctcca cagtgatcaa ggatggcatt accggataaa aagttatcag tccgccctgg
      361 cggacagagg gttagtgcag agcatgtcgc gcaagggcaa ctgccttgat aatgcggtaa
      421 tggagaactt ctttggtcac ctgaaagaag aaatgtacta tcgccgggac tacagaaatg
      481 tagaagagct ggaaaatgcc gttaacgaat acataactta ctggaatcaa aaaagaataa
      541 aactcagtct tgggggactc agcccggtag aataccggac tgagtatcaa aaagccggtt
      601 aactgaaact gtc
//
LOCUS       DAJOTB010000394          612 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJOTB010000394 DAJOTB010000000
VERSION     DAJOTB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.407"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          187 a          123 c          122 g          180 t
ORIGIN      
        1 tctaaatatg tagataatgg cgagttaacg tatcacctga cacacttctc tccgaaatta
       61 agaagtattt gactcatcaa gccaataaat gtgtatagaa tgattatacg ggatagttac
      121 aagttcaccg atacggtgca ggactcaatg gcgatgtcgg tgggagcgtt tgtgtcacga
      181 tatgatggac gtcccttctt aatgcagtca gtgccatact tttactgact gcattaatcc
      241 gagagtaaca tcccatgaac acgatcagga ttgttggtat cgatattgcc aggcctgttt
      301 ttcaggccta tatctggatt aatgatgatt ctgttgcata ggatagaaac acctcatgct
      361 ccaaattgca ggatatcgtt caacgattcg agtccggaac actattcgct atggaggcat
      421 gttcaacttc tcattctggg ggatgaacgt tcagttctat ggaatatccc ctgactattc
      481 cctgcacaac agcatcattt ttggtaaaaa aaataacatt attcttaata tatagaaaag
      541 gaactcgtcc tggacgggta caacttatta aaaatgcata tcattttcac ctcaccggaa
      601 aacgattaaa aa
//
LOCUS       DAJOTB010000395          611 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJOTB010000395 DAJOTB010000000
VERSION     DAJOTB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.408"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>611
                     /locus_tag="OHN28_005058"
     CDS             <1..>611
                     /locus_tag="OHN28_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCQ2300286.1"
                     /translation="FSVDDNGSGNVFVCGDLVNSKENIVQFNGNNNKLIIEDDVECRW
                     LTVIFRGDNNYVRIHKNSKIKGDIVATKGSKVIIGRRTTIGAGFEVVTDKCNVTIGHD
                     CMIARDVILRASDGHPIFDIHSKKRINWAKDIIISSYVWVGRNVSIMKGVSVGSGSVI
                     GYGSIVTKDVPSMCAAAGNPAKIIKRNIIWARTDKAELISDDK"
BASE COUNT          219 a           69 c          147 g          176 t
ORIGIN      
        1 ttttccgttg atgataatgg gtcaggtaat gtttttgtat gtggagatct tgtaaatagc
       61 aaagagaata tagttcagtt caatggaaac aataacaaac ttattataga agatgatgtt
      121 gagtgtcgat ggcttaccgt aatatttagg ggtgataata attacgtaag aatacataaa
      181 aacagtaaga ttaaaggtga tattgtcgca acaaaaggtt caaaagttat tatcggtaga
      241 agaacgacaa taggtgcagg ttttgaagtc gtcactgata agtgcaatgt tacaattggc
      301 catgactgca tgatagcaag agatgttatt ttgcgtgcat cagatgggca tcctatattt
      361 gatattcata gcaaaaaaag gattaattgg gcaaaagata tcattatatc tagttacgta
      421 tgggtaggga gaaatgtctc tataatgaaa ggagtatctg ttggaagcgg atctgtcatt
      481 gggtatggga gtattgtaac taaagatgtg ccatctatgt gtgcagcagc cggtaatcca
      541 gcaaaaataa taaaaagaaa tataatatgg gcaagaacgg ataaagcgga gctaattagt
      601 gatgacaaga g
//
LOCUS       DAJOTB010000396          604 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJOTB010000396 DAJOTB010000000
VERSION     DAJOTB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.409"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..188)
                     /locus_tag="OHN28_005059"
     CDS             complement(<1..188)
                     /locus_tag="OHN28_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300287.1"
                     /translation="MGATKDWVMQVEESRREEWIRERLSSPDLEEDSEEWQLLEKDYD
                     EYQDFLSDMAMEEYENEK"
     gene            complement(358..>604)
                     /locus_tag="OHN28_005060"
     CDS             complement(358..>604)
                     /locus_tag="OHN28_005060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300288.1"
                     /translation="ILWMLDVEYLPPVRATNSGLENTKKPDYQSGFFVPVVRLFFRFF
                     PSFGGFSFSFFVKSNAAGRVGQQINAVIGVFINPFFQ"
BASE COUNT          180 a          152 c          102 g          170 t
ORIGIN      
        1 catttttcgt tttcatattc ttccatagcc atatcagaaa gaaagtcctg atactcgtca
       61 tagtcctttt caagtaactg ccattcttca gaatcctcct caagatctgg gctggaaagc
      121 cgttctcgta tccattcctc acggcgactt tcctcaactt gcataaccca atcttttgta
      181 gctcccacaa taccctcctt atgttcattg ctaaatatca atagtcactt tatcggagac
      241 tacgtgaaaa atcttctccg ttccccctgc ttttttatca gatttcacgc agcgtaccgc
      301 cgctactgta tccatcaaaa aaactgatag ctttttaatc atcttttgtg ctgcaactta
      361 ctgaaaaaaa ggattaatga aaacgccgat aacggcattg atctgttggc caacgcgacc
      421 ggcagcattg gacttaacaa aaaaactgaa agaaaatccc ccaaatgaag ggaaaaaacg
      481 gaagaacagt cgaacgacag gcacaaaaaa acccgactgg taatcgggct ttttcgtgtt
      541 ttccagtcct gagttggtgg ctctgactgg aggaagatac tctacatcca acatccacaa
      601 tatt
//
LOCUS       DAJOTB010000397          592 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJOTB010000397 DAJOTB010000000
VERSION     DAJOTB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.410"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..452)
                     /locus_tag="OHN28_005061"
     CDS             complement(<1..452)
                     /locus_tag="OHN28_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCQ2300289.1"
                     /translation="MRSNITYTLDEIAEIYDGPHATPKKLETGDAIFLSISSIENGRL
                     NLEKSSFISEDDFVKWTRRVTPQAGDVVFSYETKIGEASIIPEGLRCCLGRRMGLLRA
                     KQDKVLPQYLLYAYLSPTFQDVIREKTIHGSTTDRISIREMPSFEIQLP"
     gene            complement(442..>592)
                     /locus_tag="OHN28_005062"
     CDS             complement(442..>592)
                     /locus_tag="OHN28_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300290.1"
                     /translation="VGAVEQEEDDEPFAEKMARLAIQLKDQLEDSARLEAQIKANFGG
                     LGYEI"
BASE COUNT          174 a          136 c           93 g          189 t
ORIGIN      
        1 ggtagttgta tctcgaaaga aggcatctct ctgattgaaa tacgatcagt agtactaccg
       61 tggatcgttt tttctctaat cacatcctga aatgttggag ataaataagc atataacaaa
      121 tactgaggta ataccttatc ttgtttggct ctcagcaatc ccatacgtct acctaaacag
      181 caacgtaacc cttcaggaat aattgatgct tcgccaattt tcgtttcata agaaaaaacg
      241 acatctccgg cttgaggtgt tactctacgt gtccatttaa caaaatcatc ttcggaaatg
      301 aaggatgatt tttccaaatt taatcttcca ttctcaatag aagagatact taaaaaaata
      361 gcatcccctg tctctaactt tttaggcgtt gcatgagggc catcataaat ctcggcaatt
      421 tcatcaagag tataagttat attagatctc ataacccagc cccccaaaat ttgctttaat
      481 ttgtgcctcc agtcttgcac tatcctctag ttgatctttt agctgtatag ctaaccgtgc
      541 catcttttct gcaaatggct cgtcatcttc ttcctgctca accgcaccaa ca
//
LOCUS       DAJOTB010000398          585 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJOTB010000398 DAJOTB010000000
VERSION     DAJOTB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.411"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(170..>585)
                     /locus_tag="OHN28_005063"
     CDS             complement(170..>585)
                     /locus_tag="OHN28_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296213.1"
                     /note="WbbJ; catalyzes the transfer of the O-acetyl moiety
                     to the O antigen; part of the lipopolysaccharide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2300291.1"
                     /translation="VDAFMDAVVSIGENVQINDYVHIAAINNVIIGRDTLIASKVFIS
                     DHNHGIFSKSDIHSSPTIIPSSRPLESAPVYIGERVWIGENVTILPGACIGNGVVIGA
                     NSVVRGEIPNNVIIAGVPAKIVKKYNYERMQWERI"
BASE COUNT          195 a          126 c           87 g          177 t
ORIGIN      
        1 acttctggat actctataaa ttcaacccac ggaaataatt ccattagttc agcagagcta
       61 tgaaccagtg caataaattt acattcggct ctatctttag ttgctgtaag cacatttttt
      121 agtacggtaa aggggcctcc ggttgtaaaa ttaacagccg atattacaac tatattcttt
      181 cccattgcat acgctcatag ttatattttt taacaatttt agctggaaca ccagcaatga
      241 tcacattatt aggaatctca ccacgaacaa cactgtttgc gccaataact acaccattac
      301 ctatacacgc acctggtaat attgtcacat tttcgccaat ccacacacgc tctccaatat
      361 acacaggtgc agattcaagg ggcctagacg aaggaataat agttggtgaa ctatggatat
      421 cggatttaga aaaaatacca tgattatgat cactaataaa tactttactt gctattaatg
      481 tatctctacc aataatgaca ttattaatag ccgcgatgtg aacatagtca ttaatttgaa
      541 cattttctcc aatggaaact acggcatcca taaatgcatc aactc
//
LOCUS       DAJOTB010000399          581 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJOTB010000399 DAJOTB010000000
VERSION     DAJOTB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.412"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            46..276
                     /locus_tag="OHN28_005064"
     CDS             46..276
                     /locus_tag="OHN28_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2300292.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            273..>581
                     /locus_tag="OHN28_005065"
     CDS             273..>581
                     /locus_tag="OHN28_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HCQ2300293.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     D"
BASE COUNT          129 a          164 c          171 g          117 t
ORIGIN      
        1 gcatatacta aacatatata aaagccgcct tgccaggagg aagacatgag aaccgtatct
       61 attttcaaaa atggcaataa ccgcgcgatc cgtctgcccc gcgatctgga ttttgagggg
      121 gtgagtgagc tggagattgt acgggaaggg gacagaatca ttctgcgtcc cctccgcccg
      181 agctggagct cattcctgga gctagaaaag gccgatccgg gctttatgac ggaacgcgag
      241 gacgttgtct gcgacgaagg acgatttaac ctgtgaacaa gatctatatg ctcgacacga
      301 acatctgctc gttcatcatg cgcgagcagc ctgaagcagt gcttaagaat ctggagcagg
      361 cggtgctgcg cggtcaccgt atcgtggtct cggccatcac ctactccgag atgcgcttcg
      421 gcgccaccgg tccaaaggca tcgccacgcc acgtgcagct ggttgacgcg ttctgcgccc
      481 gtctcgatgc catcctgccc tgggatcgcg ctgcggtgga tgcaaccacg gaggttaagg
      541 tggcgctgcg cctcgccggc acgccgatcg gtccaaacga c
//
LOCUS       DAJOTB010000400          580 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJOTB010000400 DAJOTB010000000
VERSION     DAJOTB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.413"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(105..580)
                     /locus_tag="OHN28_005066"
                     /pseudo
     CDS             complement(105..580)
                     /locus_tag="OHN28_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642102.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          236 a          106 c           75 g          163 t
ORIGIN      
        1 gtttatataa ccagcaccac gtataaaaag agggatgaag aaacacatgt agaagctata
       61 tgctaaaata tactgcggtg aatatctctt actttttttg aaactcagat aaaatcggga
      121 aaaataaaaa aaatagaaaa tacacattaa tgcaccaata accccaaact cagatattaa
      181 cttagaagaa ataaatgagc catcgtatat atttaacata ggggcatcaa gttcagctaa
      241 aatttgttga tatattccta tctccccatt cactcccatt tgttgaaaac caataccaag
      301 accataactt gtaataaaat tcaagaaagc tctttcaatt cctgaaagat atacaagcac
      361 tgataggttc gtagtatttt taaaatcaag ccgcgatgta tagtaagaaa tgtctaaatc
      421 acttaatatc atggaaaaaa tccatgcccc caaaagtata acaatagttt gccttatagt
      481 tatttttttc atcacaaaaa cactaatcac aatgcctacc aacattgtta aattctggat
      541 acctaacgca atacccaaag ataatatata gagcaatagt
//
LOCUS       DAJOTB010000401          571 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJOTB010000401 DAJOTB010000000
VERSION     DAJOTB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.414"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>571)
                     /locus_tag="OHN28_005067"
     CDS             complement(<1..>571)
                     /locus_tag="OHN28_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HCQ2300294.1"
                     /translation="CLDIAYGTDKNFLFGCGISIASILKYNEGSRLCFHIFTDYFGDN
                     DRKYFDALALQYKTRIKIYLINGDRLRSLPSTKNWTHAIYFRFVIADYFISKVAKVLY
                     LDADIICQGTIEPLIKFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGISKGYFNSGFL
                     LINTAKWAAQQVSARAIAMLNEPEVIKKIT"
BASE COUNT          191 a          121 c           93 g          166 t
ORIGIN      
        1 gttatttttt tgattacttc tggctcattt agcattgcaa tggctcgtgc agaaacctgc
       61 tgagctgccc atttggcagt attaatcaat aaaaaaccgg agttaaagta acctttagaa
      121 attcccgcaa cacctaacga atgggcgcgt ttttcccacc agtcagcttg cccttctgta
      181 acaaccattg cgactttatc gtcaggaaat gagaatttaa ttagtggctc aatagtcccc
      241 tgacaaatga tatctgcatc cagataaagg actttagcca ccttactaat aaagtaatca
      301 gcaataacaa aacgaaaata tattgcatga gtccagttct ttgtgctagg caatgagcgc
      361 agtcgatcac cattaatcag atatatttta attctggttt tatactgtaa cgccagggca
      421 tcaaaatact tacgatcatt atcaccaaaa taatcagtaa aaatgtgaaa acacaaccga
      481 cttccttcat tatactttaa tattgaagca atcgaaattc cgcaaccaaa tagaaagttt
      541 ttgtcagttc cataagcgat gtccagacaa a
//
LOCUS       DAJOTB010000402          542 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJOTB010000402 DAJOTB010000000
VERSION     DAJOTB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.415"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          194 a           90 c          103 g          155 t
ORIGIN      
        1 aataatcaca agaagaaata gataccttag gtatagtcag atggaacaag gattttcaaa
       61 ataaatttat atggtattaa tatttcccga tctgtattca atgattcgct caaagattga
      121 aaatcaagag aaattgttat agcagtgata cgagacaaca aaacggaatg attgccagcc
      181 gttagaaaac accggacact ctcgtgtaaa aagctaccct gtctatggat atagcgatat
      241 cgactcatca agcgtggact gaatgatatt cagctccata tgggcggcaa cctggaggat
      301 aaacatgatt tcgccgttgg aatcaaggtt gttctgcaat atctaccaga ctgcttcaat
      361 ttatctgttt tggaagtgca ataatcttaa tttaactatt tgaagatgat gatattgtca
      421 gatgccagaa atttagtaat ctggagaacc gtgtctatga tcactaaata ttaaagtaat
      481 agtcgtcagt aacacatcga gtgatataac gtattgacac ctaatcatat aaaaacagat
      541 cg
//
LOCUS       DAJOTB010000403          511 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJOTB010000403 DAJOTB010000000
VERSION     DAJOTB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.416"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(154..342)
                     /locus_tag="OHN28_005068"
                     /pseudo
     CDS             complement(154..342)
                     /locus_tag="OHN28_005068"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          138 a          114 c           95 g          164 t
ORIGIN      
        1 gcactataag ttatcctctt tactttatgc ttaccaactc atttatgtta ctttataaca
       61 taacccatct tgcgttgtaa acagactcgt tttagttcca tgcacctttt gagatcgtga
      121 ccaaataatg gcgttagtga tgttcttcca gtgtcatatg gttaagtagt tgatatggcg
      181 ttcactgtta tattctgaca accagtttgc cgccagttcc cgcttttcat ccagcgttct
      241 gaacagacaa aaaccgagta tttttgttcg gtatgtccgg ttaaaactct cgataaaagc
      301 gttctgcgcc aacttacctg attgggtaat ctccagtttt actacatgtt tttctgcgca
      361 ttacgtccgt gttggataaa aaaaatcctg gccataatct atgcgaatca tagctggata
      421 gacatggttt tccgcaatcc ttgaaccgcc ccgggaatcc tggagactaa actccctgag
      481 aaagaggtaa acaggatgac taaaaatact c
//
LOCUS       DAJOTB010000404          508 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJOTB010000404 DAJOTB010000000
VERSION     DAJOTB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.417"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(390..>508)
                     /locus_tag="OHN28_005069"
     CDS             complement(390..>508)
                     /locus_tag="OHN28_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCQ2300295.1"
                     /translation="FYQYFKISNTGIDHLEQLINVNVFSSDRMSFCECNRFP"
BASE COUNT          155 a          101 c           94 g          158 t
ORIGIN      
        1 tcgttgaata atttgggcgt tataacctaa atttttaccg tccaataatc aatatttcta
       61 ttgtgattgt taaccactgc gtgcattgac gggattgaag ggtgaagaat atgtgcaatt
      121 gagaagcctt tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattc
      181 tccttgcacg gttctttcgc actatcaaaa aacgctcatt tacccggcat tacagtcaac
      241 gaagcaacat tctatggcct acgctgatgt aaagcacagt gagaatcccc cttgttgtct
      301 tcgtctgacc ttatcgcggc gaccagcaat tattcgtttt gtgatcctca acacaaaatg
      361 ctcttcgttg cctatgatgg gagaagaatt tatggaaaac gattgcattc acaaaatgac
      421 atcctgtcag aagagaaaac gttgacgtta atcaattgtt cgagatgatc aatacctgta
      481 ttcgaaattt taaaatattg ataaaata
//
LOCUS       DAJOTB010000405          498 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJOTB010000405 DAJOTB010000000
VERSION     DAJOTB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.418"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(297..>498)
                     /locus_tag="OHN28_005070"
     CDS             complement(297..>498)
                     /locus_tag="OHN28_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCQ2300296.1"
                     /translation="VSGVAGKSTVYQLAAWALKENGEVVGLVTVRDDNGRPKLVTPPP
                     VPGDYLHKEQLTDDEKEWAKRR"
BASE COUNT          174 a          101 c           80 g          143 t
ORIGIN      
        1 tggtgattat gttaaaattg gaaactacct aaaaacaatt gtaacatctt atctgctatc
       61 agatttagga atcaataaca ttattattga aaaatatcag gctcagacaa ttcaagagta
      121 ataaaatatt atacagttac aattgtcatt taaatataat aagacgagaa acgagcaacg
      181 gaaaagtaaa atcaatatca atttacatca atttgtgcat tgaagccatt atcaactcca
      241 gtgcaactaa acatgctaat aaagcctcta gaacagaggc tttatatcaa tatagtttag
      301 cgtctcttcg cccactcttt ttcatcatcg gtgagttgtt ctttatgcaa ataatcacca
      361 gggacagggg gaggagtaac cagtttagga cgcccattat catcacgaac cgtcactaga
      421 ccaactacct caccattttc tttaagcgcc catgcagcca gttggtatac tgttgacttt
      481 cctgccacac cggagaca
//
LOCUS       DAJOTB010000406          487 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJOTB010000406 DAJOTB010000000
VERSION     DAJOTB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.419"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..487)
                     /locus_tag="OHN28_005071"
                     /pseudo
     CDS             complement(1..487)
                     /locus_tag="OHN28_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491457.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
BASE COUNT          160 a           97 c           95 g          135 t
ORIGIN      
        1 caaataagag gacgattgtc ggcgctgggg ctctgttgag aaaatataat aaccatacgc
       61 ctgatattgt tattgatatc gttacggcga taagtaatgc cttatgaaag cgatgcatga
      121 tgaggacagc aatagaccct ggagctatta atagtatata gacagaataa ttccgactgc
      181 ttttaatgtg gcaaaaatgg taggcgatac catgcagagt aaaccgtaat gtaataatga
      241 tatctgtaat ccgctgactt gtgcctgttg atagtcaaga caaaaattcc tgaaaaaacg
      301 atacctataa tggtatcttt ctttataatg ctattatctt tcagatagcc agttgcaatt
      361 gcacaagaca gtcctacaat aaatgcacca attcccagcg gcatacctat aatccagact
      421 agaactatgc caggaaacat tgcgtggctc attgcatctc ctattagcgc ccagcctttt
      481 aatacca
//
LOCUS       DAJOTB010000407          479 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJOTB010000407 DAJOTB010000000
VERSION     DAJOTB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.420"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>479
                     /locus_tag="OHN28_005072"
     CDS             <1..>479
                     /locus_tag="OHN28_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300297.1"
                     /translation="IKKYLDGIAESSENPKEKKLASLFGMLYSFNLQVVNNTPSFEPQ
                     MIWGNKRSILPEDFDEQVNDCLLYVSQKITNPFLLSRIYDVVWCNNRKNKDVAIKAID
                     SYAEMLNIAIEKLIIKNESMNESDLNCFIFARDYIARALHINKMVYPRKSEGNMAIK"
BASE COUNT          184 a           64 c           82 g          149 t
ORIGIN      
        1 ataaaaaaat atctggatgg gattgctgaa tcatcagaaa accccaaaga gaaaaaatta
       61 gcatctcttt ttggtatgct atatagtttc aatttgcaag ttgttaacaa cactccatca
      121 tttgaacccc aaatgatttg gggtaataaa agatcaatat tgcctgaaga ttttgatgag
      181 caggtgaatg actgtctttt atatgtttct caaaaaataa ccaatccatt tttgttatca
      241 agaatatatg atgttgtttg gtgtaataac cggaaaaata aagacgtggc aattaaagca
      301 attgattcct atgctgaaat gctaaatata gcaattgaaa aactaataat aaaaaacgaa
      361 agcatgaatg aaagtgattt aaattgcttt atttttgctc gtgattatat tgctagggct
      421 ttgcacatta ataaaatggt ctaccctaga aaatcagaag gaaacatggc gattaaaaa
//
LOCUS       DAJOTB010000408          464 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJOTB010000408 DAJOTB010000000
VERSION     DAJOTB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.421"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          144 a           82 c           76 g          162 t
ORIGIN      
        1 atatcatcag gatgggcacc aattgctaaa atgccggttc tttttcccga agatgttgta
       61 gaattcaaca tgtaatctat ccttttagat atgccatatg aatccccatc ataaattatc
      121 actatcataa aaattaatct tattaaatat actttatatt tactttttca gaggcagaga
      181 agcatcattt atgcaacaag aagatcttca tttctgttaa aaaaacagag tttatatgct
      241 attacggatt tcagtcgtag caagttgaac tgtgatcgca ttaagactaa tcttctggct
      301 tgtaaaggcc ttagttccgt catgactctc ccatcaggcc atagttgagc aaaatcctga
      361 aagaattatc acctattgta tcttcagata cgggagtttt tgtccagtat tgttggaata
      421 atcatgtgcc tatattgttc ttgtagttgg aacgtcttat aaat
//
LOCUS       DAJOTB010000409          461 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJOTB010000409 DAJOTB010000000
VERSION     DAJOTB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.422"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          167 a           89 c           66 g          139 t
ORIGIN      
        1 ctcaatataa catattgatt caggtgggat attaataacc cccccatcta tcgttttaac
       61 aagtaattgg gcattgcgaa gataaataat ggcacaattg tagaatctaa tctttttaag
      121 aaccaatgta gagttaccgg atatatcccc tctcacagca agcctcatat tacttaaagc
      181 attttgacta ttcatacagt cacccccatg ctttgtaaca acataatttc atgggatatc
      241 cactctgtaa actcggaaat aacgcaaaga tgaatacttc ccggtaaaaa gtttcgcatc
      301 gacatatcaa ctaatttcat agactataaa taattagtca ctatgatgat aaaattaatt
      361 gactctgata atagttacgt catgaaaata aaatcagttg atgttacaga taacacctaa
      421 aaagaattgg aagcttccgt gtcgtttcag caccaaattt a
//
LOCUS       DAJOTB010000410          460 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJOTB010000410 DAJOTB010000000
VERSION     DAJOTB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.423"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>460
                     /gene="rfaJ"
                     /locus_tag="OHN28_005073"
     CDS             <1..>460
                     /gene="rfaJ"
                     /locus_tag="OHN28_005073"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase RfaJ"
                     /protein_id="HCQ2300298.1"
                     /translation="QKVAKLAEQYQLRITLYRINTDKLQCLPCTQVWSRAMYFRLFAF
                     QLLGLTLDRLLYLDADVVCKGDISQLLHLDLNGAVAAVVKDVDPMQEKAASRLSDPEL
                     LGQYFNSGVVYLDLKKWANAKLTEKALSILMSKDNVYKYPDQDVMNVLLKG"
BASE COUNT          142 a           76 c           97 g          145 t
ORIGIN      
        1 caaaaagttg caaagcttgc agagcaatac caattaagaa tcacattata tagaattaac
       61 actgataagc tccagtgctt gccttgtaca caggtctggt caagagcaat gtacttccgt
      121 ttatttgcat ttcaattatt gggtttaaca cttgatcgtt tgctttatct tgatgcagat
      181 gttgtttgta aaggcgatat tagccaacta ttgcatctgg atttaaacgg cgcggttgct
      241 gctgttgtta aagatgttga cccaatgcag gaaaaggcgg catccaggtt gtctgatcca
      301 gaattactcg ggcagtactt taattccggt gtcgtttatt tagatttaaa aaaatgggct
      361 aacgcaaaac taacagaaaa agcgttgtct attcttatga gtaaagataa tgtgtataaa
      421 taccctgatc aagatgttat gaatgtctta ctgaaaggga
//
LOCUS       DAJOTB010000411          452 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJOTB010000411 DAJOTB010000000
VERSION     DAJOTB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.424"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(16..>452)
                     /locus_tag="OHN28_005074"
     CDS             complement(16..>452)
                     /locus_tag="OHN28_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300299.1"
                     /translation="NKEKFITLGAINKMAFELEKDEYKRKLEAGKYSLNPDDHKHSLE
                     EYCDDLRKSILASVLIMLGVLFIVIAIGFYSGKFRIIYPLEWKDAVSATGIFILSWST
                     LFQLGWRNASWKGCRLDELVGLAIFRRFFIFGSFLSLLYFAI"
BASE COUNT          146 a           98 c           71 g          137 t
ORIGIN      
        1 ttttaaaatc ctgtattata ttgcgaagta taataagcta aggaacgagc cgaaaataaa
       61 aaaacgtcta aatattgcta atccaaccag ttcatcaagg cgacagcctt tccatgatgc
      121 attcctccag cctagctgaa acaatgttga ccatgataga ataaatattc cggtggcgct
      181 aacggcgtct ttccattcta atggatagat aatccgaaat ttccctgagt aaaatccaat
      241 cgcaattact ataaaaagca cacccaacat tattagcact gacgcaagaa tggactttct
      301 taagtcatca caatactctt cgagggagtg tttgtgatca tctggattta gactgtattt
      361 ccccgcctca agttttctct tatattcatc cttctcaagt tcaaaagcca tcttattgat
      421 agcaccaagc gtaataaatt tttccttgtt ga
//
LOCUS       DAJOTB010000412          440 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJOTB010000412 DAJOTB010000000
VERSION     DAJOTB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.425"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>440)
                     /locus_tag="OHN28_005075"
     CDS             complement(<1..>440)
                     /locus_tag="OHN28_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCQ2300300.1"
                     /translation="KLHLTSTEEYKKRVIQLGEKPGSVFNIGSLGAENALSLHLPNKQ
                     ELELKYGSLLKRYFVVVFHPETLSTQSVNDQIDELLSAISFFKNTHDFIFIGSNADTG
                     SDIIQRKVKYFCKEYKFRYLISIRSEDYLAMIKYSCGLIGNSSSG"
BASE COUNT          153 a           82 c           68 g          137 t
ORIGIN      
        1 ccagaggagg agttcccaat tagcccacaa gagtatttaa tcattgccaa ataatcttct
       61 gaacgaatag aaatcaaata tctgaactta tactctttgc aaaaatattt tactttcctc
      121 tgaattatat cagaaccagt gtcagcgtta ctgccaataa aaataaagtc gtgagtattt
      181 ttaaaaaaag aaatcgctga caataactca tctatttgat cattaaccga ctgcgtggaa
      241 agtgtttcag gatggaatac tacaacaaag taccgtttta acagtgaacc atattttagt
      301 tccaactcct gcttatttgg taaatgcaat gaaagagcat tttctgcacc aagagaacca
      361 atattaaaca cactaccagg cttttcacct agttgaatta ctcgtttttt atactcttct
      421 gtagaagtaa gatggagttt
//
LOCUS       DAJOTB010000413          436 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJOTB010000413 DAJOTB010000000
VERSION     DAJOTB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.426"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..246
                     /locus_tag="OHN28_005076"
     CDS             <1..246
                     /locus_tag="OHN28_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HCQ2300301.1"
                     /translation="NNTIFIHYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTALLK
                     PNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFIEKIKH"
     gene            286..>436
                     /locus_tag="OHN28_005077"
     CDS             286..>436
                     /locus_tag="OHN28_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCQ2300302.1"
                     /translation="MDSFPAIEIDKVKAWDFRLANINTSECLNVAYGVDANYLDGVGV
                     SITSIV"
BASE COUNT          149 a           71 c           83 g          133 t
ORIGIN      
        1 aacaatacta tttttatcca ttatatcggg ccaaccaagc cctggcatga ttgggcctgg
       61 gattatcctg tatcacaagc ctttatggag gcaaaaaatg cttcgccatg gaagaataca
      121 gcgttgctga aaccgaacaa tagcaatcaa ttaagatata gcgcaaaaca tatgcttaaa
      181 aaacatagat atctaaaagg atttagcaac tatctttttt attttattga aaagataaag
      241 cattaaaact gggaaacaat aaagtaatga tataagggta gcattgtgga ctcatttcct
      301 gccatagaga tagataaagt taaagcctgg gattttcggc tggctaatat aaatacttct
      361 gaatgtttaa atgttgccta tggtgtcgat gctaattatc ttgatggtgt aggtgtttcc
      421 attacatcaa ttgtcc
//
LOCUS       DAJOTB010000414          415 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJOTB010000414 DAJOTB010000000
VERSION     DAJOTB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.427"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          147 a           82 c           54 g          132 t
ORIGIN      
        1 atgaccaaac atcaatgcca ttatactcgc ctctgttaaa tactcgctag ttaatatgat
       61 aggcacgcat gatataacaa tccatatgct aatacataaa tacatcagta tttttctcat
      121 agtcagtccc tgtgattaac aatcaaggca tagttaagca tatttcacat tcaaacgtaa
      181 acattattaa taacattata tcaatcgaac tgacatcacg attcaagccc tcttcaaact
      241 gaaggttaga gcagcgtaga acatttcaaa acaatacact aaaaaaatac ctgaacaaaa
      301 aacttaattc ttttttattg tgaactttag tgattttgtc atgcaactct ttattacatt
      361 taggcctgta ttatttcatg gcaacgcaga tacaggacca tttcttgtgc tatgg
//
LOCUS       DAJOTB010000415          408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJOTB010000415 DAJOTB010000000
VERSION     DAJOTB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.428"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(265..>408)
                     /gene="tuf"
                     /locus_tag="OHN28_005078"
     CDS             complement(265..>408)
                     /gene="tuf"
                     /locus_tag="OHN28_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2300303.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLG"
BASE COUNT          146 a           85 c           74 g          103 t
ORIGIN      
        1 aaaacaaaag tcctacgcag gaattattta cgttttacga gaacccttca atatgaatta
       61 aagtgcggca ttaaaaataa gaaaaaagcc tgacaaatga agcattttta aaacagaaac
      121 attcatattt aaaatattaa attgaattga tattttaaat atgaataagt tattcgttct
      181 gacagtacga ataagatatg cagtcaacaa atgcaaaaag ggcgccgaag cgcccttttc
      241 aattcaaaac taattaacgt gtaattagcc cagaacttta gcaacaacgc ccgcgccaac
      301 ggtacggccg ccttcacgga ttgcgaaacg cagaccgtcg tccatcgcga tcgggtggat
      361 cagggtaaca accattttga tgttgtcgcc cggcattacc atctctac
//
LOCUS       DAJOTB010000416          407 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJOTB010000416 DAJOTB010000000
VERSION     DAJOTB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.429"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>407
                     /locus_tag="OHN28_005079"
     CDS             <1..>407
                     /locus_tag="OHN28_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HCQ2300304.1"
                     /translation="FFYPNALPAQVTYVELLDTDGIYYQFRTLDATAPSSTTIGQWRD
                     NLRVGVLSFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGEVWKLMLTLYSPPGGGSE
                     KYYLRYLLIGLAPEGKIGVWLEKPDKPNIRLTD"
BASE COUNT          111 a           84 c           99 g          113 t
ORIGIN      
        1 tttttttcta ccctaatgcg ctaccagcac aagttaccta tgtagaacta ttggacactg
       61 atggtatcta ttatcagttc agaacgctgg atgctaccgc tccgagttcc actactattg
      121 gtcagtggag agataatctt cgggttgggg tgttatcatt caataaagcc aaaaatcccc
      181 ctatgtctat ccatttctgc tgggattcag tgattgataa aaaagtgtat gaaacctgga
      241 taacgctggc gggagaagtc tggaaattga tgctcacact ttattcacca ccaggcggtg
      301 gcagtgaaaa gtattatctg cggtatctgt taatcgggct ggctccggaa gggaaaatag
      361 gagtgtggct ggagaaacct gacaagccca atattcgcct gacggat
//
LOCUS       DAJOTB010000417          406 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJOTB010000417 DAJOTB010000000
VERSION     DAJOTB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.430"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          126 a           74 c          104 g          102 t
ORIGIN      
        1 tctttcggtg tcgcctctgg cttttccacc gaaaacaatt acacaactac agagaataac
       61 gagagttatt aaatgtagaa agtggaattg tattgaggct tagtgaaatt acacgaggaa
      121 agcagagtgt aattgagctt tagccgctct ctcttcgagt aattacacaa ctacagaatg
      181 tagaagtgga attaagaggt agagagagag ttaacacata agcaatcagt atgattggtt
      241 aggtgattac tcttactgcg cacagcgcaa tagtatttct tcgtgggcgt tatcgccaac
      301 gatagttaca cgaagaaagt aacgctttgc tttcggtgtg agcggaacgg gccagccacg
      361 aagcccaaag gtagcggagt aaggctgctt gccagagagt gaagct
//
LOCUS       DAJOTB010000418          403 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJOTB010000418 DAJOTB010000000
VERSION     DAJOTB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.431"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            143..>403
                     /locus_tag="OHN28_005080"
     CDS             143..>403
                     /locus_tag="OHN28_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCQ2300305.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYK"
BASE COUNT          136 a           62 c           83 g          122 t
ORIGIN      
        1 atgataagcg caggccgagc aatttaaaga agtgtgatat acaacataaa acagcagcat
       61 gacctttcta tatttcgttg cttatcgaag tgttaatttt cgggtggatt actctgtttg
      121 ttgattaata acggagaaaa atatgattgc tgtaatattt gaggtgcaaa tacaacccga
      181 ccaacaaact cgctatttga ctttagctga ggagttaaga ccactattaa gtcatgtagc
      241 tggttttatt tcaattgaac gttttcaaag tctagctaca gaaggaaaaa tgttatcgct
      301 atcttggtgg gaaaacgaat acgcagttct gcaatggaaa aatcatgttt tacatgcgaa
      361 agctcaacaa gaagggcgag agtcaatatt tgatttttac aaa
//
LOCUS       DAJOTB010000419          397 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJOTB010000419 DAJOTB010000000
VERSION     DAJOTB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.432"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..320)
                     /locus_tag="OHN28_005081"
     CDS             complement(<1..320)
                     /locus_tag="OHN28_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCQ2300306.1"
                     /translation="MLRETPEIQLDLAVTGMHCDNAYGNTIHIIEQDNFNIIKVVDIN
                     INTTSHTHILHSMSVCLNSFGDFFSNNTYDAVMVLGDRYEIFSVAIAASMHNIPLIHI
                     HGGE"
BASE COUNT          132 a           71 c           54 g          140 t
ORIGIN      
        1 ttttcaccac catgaatatg aattaatgga atattatgca ttgatgctgc gatagcgact
       61 gaaaatattt catatctatc gcctaaaacc ataaccgcat catatgtgtt atttgaaaaa
      121 aaatcaccaa acgaattgag gcaaacactc attgaatgga gaatgtgagt atgtgaagtt
      181 gtattgatat ttatatccac aaccttgata atattaaaat tatcttgttc tataatatgt
      241 attgtatttc catacgcatt atcacaatgc attcctgtaa ctgccaaatc aagctgtatt
      301 tctggagttt ctcttagcat tgtcaaaagt ctccgaacta ttccatattc agctctagat
      361 ccagttacgt ataatatttt tttcatttaa caatctc
//
LOCUS       DAJOTB010000420          394 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJOTB010000420 DAJOTB010000000
VERSION     DAJOTB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.433"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>394
                     /locus_tag="OHN28_005082"
     CDS             <1..>394
                     /locus_tag="OHN28_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2300307.1"
                     /translation="VMEPLLLNPHKIGPMRSRIFNCMADKTDTEIFKMITEANVPNHR
                     LIYNITYLLSKVDDIESLVCSLSVSTDSTFTEKLKLIIESDLSKSWRLVDLANVLHMS
                     EVSIRKKLEKENNNFNNLILDIRMQHAAR"
BASE COUNT          147 a           56 c           63 g          128 t
ORIGIN      
        1 gtcatggaac cattgttact gaatccccat aaaattggcc ctatgcgaag tagaattttt
       61 aattgcatgg cggataagac agatacagaa atatttaaaa tgataacaga ggcaaatgtc
      121 ccaaatcata gactaattta taacattact tatttgttat ctaaagttga cgatattgaa
      181 tctttggttt gctcattatc tgtttcaaca gactctactt tcacagaaaa attaaagtta
      241 ataatagaat ctgatctgtc aaaatcatgg cgattagttg atctggcaaa tgttttacat
      301 atgtctgaag tctcaattcg gaaaaaactt gaaaaagaaa ataataattt taacaatttg
      361 attttggata ttagaatgca acatgcagca agat
//
LOCUS       DAJOTB010000421          386 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJOTB010000421 DAJOTB010000000
VERSION     DAJOTB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.434"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          127 a           55 c           86 g          118 t
ORIGIN      
        1 aacagcatta gtttgatgat tttatactga actaaagggg gaaagaggta acgaaaaaag
       61 acctccacca ggtacgaatc tgaggcgggc agatggaggt cgatatctac ccgattaaat
      121 ggataagtaa aagccccatc agggattgat ttgttcgagt ctaatttaaa gtaatttcaa
      181 cgatttggca gaacttctta gagttaatga ttttcaagtt ttcgattacc aggaaaaagg
      241 ctgggggctg ataaaatagt catctgcatt ccttaaattg tgtaagggtg aaaggataaa
      301 gttcaattta aaggcggtac aatattagca ccatcttata tatgtaatga ttgatttgcc
      361 tctttattct actgttgaga ttattg
//
LOCUS       DAJOTB010000422          386 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJOTB010000422 DAJOTB010000000
VERSION     DAJOTB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.denovo.435"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..361
                     /locus_tag="OHN28_005083"
     CDS             <1..361
                     /locus_tag="OHN28_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2300308.1"
                     /translation="YDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKWYFQR
                     LFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKYIFKV
                     TPSYYRRNKLWELEAMH"
BASE COUNT          121 a           65 c           80 g          120 t
ORIGIN      
        1 atatgatgat aagcagtttc atacaacggt tatcaaagat gttctattat ggattgaaca
       61 taatttagat cagtctttac tgcttgatga tgtggcgaat aaagcgggtt ataccaagtg
      121 gtattttcag cggctgttca aaaaagtaac aggggtcaca ctggctagct atattcgtgc
      181 tcgtcgtttg acgaaagcgg ctgttgagtt gaggttgacg aaaaaaacta tccttgagat
      241 cgcattaaaa tatcaatttg attcccaaca atcttttaca cgtcgattta agtacatttt
      301 taaggttaca ccaagttatt atcggcgtaa taaattatgg gaattggagg caatgcactg
      361 agagatcccc tcataatttc cccaaa
//
LOCUS       DAJOTB010000423         4594 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.253, whole genome shotgun sequence.
ACCESSION   DAJOTB010000423 DAJOTB010000000
VERSION     DAJOTB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.253"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1423)
                     /gene="pepD"
                     /locus_tag="OHN28_005084"
     CDS             complement(<1..1423)
                     /gene="pepD"
                     /locus_tag="OHN28_005084"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCQ2300309.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTL"
     gene            1684..2142
                     /gene="gpt"
                     /locus_tag="OHN28_005085"
     CDS             1684..2142
                     /gene="gpt"
                     /locus_tag="OHN28_005085"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2300310.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2234..3478
                     /gene="frsA"
                     /locus_tag="OHN28_005086"
     CDS             2234..3478
                     /gene="frsA"
                     /locus_tag="OHN28_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCQ2300311.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPCLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3536..3937
                     /gene="crl"
                     /locus_tag="OHN28_005087"
     CDS             3536..3937
                     /gene="crl"
                     /locus_tag="OHN28_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCQ2300312.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(3976..>4594)
                     /locus_tag="OHN28_005088"
     CDS             complement(3976..>4594)
                     /locus_tag="OHN28_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2300313.1"
                     /translation="RASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFG
                     TSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFY
                     SETRKMTPISGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYV
                     DVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1100 a         1129 c         1180 g         1185 t
ORIGIN      
        1 gcagtgtcca gtaatggcca acgctttcga tatgaacttg ctcatccggg gagtgtggac
       61 cggtgatggt tggcccgata gacaccatgt ccatttccgg atacggtttt ttgaacagac
      121 cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac agacgctgat
      181 aggtttcacg taccagatgc attaccggag agttagcgtc cggctgccag ccaggatatg
      241 cgccttttgc ttcggttttc gcacctgcca gtttacccag cgaatccagc atgctcacta
      301 cgtagtcttt accgctgtcg atcagagaac ggatcaggca gtgaatttct acgttattgt
      361 cggtcatggt caccacaccg acgttcaggg aggtttcaac cacgcctttg gctacatcgg
      421 aattacggat aactccgttc ggggtggcgt tcagcagacg aataaaggta tcgcgagatg
      481 tcgccgttaa tgcagctttg tcgttcgcta cggagtccag cagcaatgcc agattcttct
      541 ctttttctgc cagttcgttt ttcaggatct cctgataggt atccaccaga gatttcaggg
      601 cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac
      661 gcagtgtgcc gccgttgaaa tcaataagac gcaggtccag ttcttctgca tgacccgcca
      721 ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca ccgccggagt
      781 gaccgccttt cagacctttt aaggttaaat tgaaggtttc aaaaccagct ggaaccgctt
      841 cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacatccc atgtagattt
      901 caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag ttgctctgta
      961 agccgaacgc accgtccata ccggcttctt cagtcatggt cagcagcact tccagcgggc
     1021 cgtgaaccac gttttcgtca gccagaaccg ccagtgcgga ggccatacca atgccgttat
     1081 ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa ggctggatag
     1141 gatctttagt gaagtcatgc acggtgtcgt tatttttttg cggcaccata tcgaggtggg
     1201 cctgcaagac gaccggttta cgattttcca tacctgcggt agcaggttta cgaatcagga
     1261 tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa ccaacaatgt
     1321 attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt ttggcaaaaa
     1381 tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt ctccttgtcg
     1441 atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt caacacaaga
     1501 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     1561 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     1621 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     1681 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     1741 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     1801 ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     1861 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     1921 gatggcgaag gcttcatcgt tattgatgac ctggtagata ccggtggtac tgcggttgcg
     1981 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     2041 ccgctggttg atgattatgt tgttgatatc ccgcaaaata cctggatcga acagccgtgg
     2101 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     2161 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     2221 ggaggctgca tctatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     2281 acatcctgaa acctcgacgc tagtccgccg ctttagtcac ggcgcacaac tgcctgtgca
     2341 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     2401 gatctggcgc ggcattgacc cccgtgaaat cctcgaagtc caggcgcgta ttgtgatgag
     2461 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gcggcggcaa
     2521 ctggatttat gagtgggcta cccaggcgat ggtgtggcaa caaaaagcct gtgcggaaga
     2581 agatccgcaa ctcagcggtc gacactggct gcatgcggct acgttgtaca acattgctgc
     2641 ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttgt caaaccgcgc
     2701 ctatgaagag gccgctcagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc
     2761 cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac
     2821 agtattaatg tgtggtggtc tggatgcgat gcagacagac tattacagcc tgtatgaacg
     2881 ttattttgcg ctgcgcggca ttgcgatgct gactattgat atgccgtcgg tgggcttttc
     2941 ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgttt taaaggcgtt
     3001 gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc
     3061 taacgttgcc gtacgtctgg cataccttga atcgccgtgt ctgaaagcgg ttgcctgtct
     3121 tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat
     3181 gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttctgacg aagcgttgcg
     3241 cgtggagctg aatcgctatt cattaaaagt gcaagggttg ctgggacgtc gctgcccaac
     3301 gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg actcacgctt
     3361 aatcacgtcg tcatctgctg acggtaaatt attagagatc ccatttaacc cggtgtatcg
     3421 gaattttgac aaaggccttc aggaaatcac agactggatc gaaaaacgtt tgtgttaaaa
     3481 atttgctaaa ttttaccaat ttggtaaaac agttgcatca caacaggaga ttgcaatgac
     3541 gttaccgagt ggacacccga agagcagatt agtcaaaaaa tttaccgcac taggcccgta
     3601 tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg ctgtatgcgt
     3661 caacgtgaaa ccggcaccgg aagtgcgtga gttctggggc tggtggatgg aactggaagc
     3721 gcaggaatcc cgttttacct acagttacca gtttggtctg ttcgataaag caggcgactg
     3781 gacgagtgtt cagataaaag atgccgaagt ggttgaacga ctggagcaca ccctgcgtga
     3841 atttcacgag aagctgcgtg agctgctggc gacgctgaat ctgaagctgg aaccggcaga
     3901 tgattttcgt gatgaaccgg tgaagttaac ggcgtgaatg aaatgtgccg gatgcatcac
     3961 atccggcaat atccattaaa actgataggt catgccaacc gcgacaatat catcattatt
     4021 aatattcaat ttgttatcgc tatccagttg gttgatttta taatcaacaa acgctgacat
     4081 atttttgttg aaataatacg tagcaccaac atcgacataa ttgaccagat cttcatcacc
     4141 gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac gcagaccaaa
     4201 gtcaaactgg tattgagcga ccgcttcaaa gttctgcgtc ttattggcaa agccgccaga
     4261 gattggcgtc attttacgtg tttcagaata gaaagttgcc agataaatat tattggcatc
     4321 gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc tttgcaggtt
     4381 ctgctcgttg gtgcgatcag agttggtata ggctccactg atggcgaaat cgctgccacc
     4441 aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt
     4501 ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc cgaagaagtc
     4561 ggtgttccga tacgtcgcca gaccgctggc gcgt
//
LOCUS       DAJOTB010000424         3606 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.276, whole genome shotgun sequence.
ACCESSION   DAJOTB010000424 DAJOTB010000000
VERSION     DAJOTB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.276"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            76..936
                     /gene="aac(3)-IId"
                     /locus_tag="OHN28_005089"
     CDS             76..936
                     /gene="aac(3)-IId"
                     /locus_tag="OHN28_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HCQ2300314.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            949..1491
                     /locus_tag="OHN28_005090"
     CDS             949..1491
                     /locus_tag="OHN28_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2300315.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQL
                     LHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDS
                     GAHTSDELAAMVLDGIDGRT"
     gene            complement(1973..2164)
                     /locus_tag="OHN28_005091"
     CDS             complement(1973..2164)
                     /locus_tag="OHN28_005091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300316.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     gene            2188..2415
                     /locus_tag="OHN28_005092"
     CDS             2188..2415
                     /locus_tag="OHN28_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300317.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     gene            2466..3593
                     /locus_tag="OHN28_005093"
     CDS             2466..3593
                     /locus_tag="OHN28_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HCQ2300318.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRD
                     PKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRIIRIGEDWS
                     EEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQNISAVLDA
                     LGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVE
                     PPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPEL
                     FQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAG
                     DQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTGTVANSRW"
BASE COUNT          738 a         1030 c         1059 g          779 t
ORIGIN      
        1 acttaatgat ttttaccaaa atcattaggg gattcatcag cgtatagtgt tttgcagttt
       61 agaggagata tcgcgatgca tacgcggaag gcaataacgg aggcgcttca aaaactcgga
      121 gtccaaaccg gtgacctctt gatggtgcat gcctcactta aagcgattgg tccggtcgaa
      181 ggaggagcgg agacggtcgt tgccgcgtta cgctccgcgg ttgggccgac tggcactgtg
      241 atgggatacg cgtcgtggga ccgatcaccc tacgaggaga ctctgaatgg cgctcggctg
      301 gatgacgaag cccgccgtac ctggctgccg ttcgatcccg caacagccgg gacttaccgt
      361 gggttcggcc tgctgaatca atttctggtt caagcccccg gcgcgcggcg cagcgcgcac
      421 cccgatgcat cgatggtcgc ggttggtccg ctggctgaaa cgctgacgga gcctcacgaa
      481 ctcggtcacg ccttggggga aggatcgccc gtcgagcggt tcgttcgcct tggcgggaag
      541 gccctgctgt tgggtgcgcc gctaaactcc gttaccgcat tgcactacgc cgaggcggtt
      601 gccgatatcc ccaacaaacg gtgggtgacg tatgagatgc cgatgcttgg aagagacggt
      661 gaagtcgcct ggaaaacggc atcggattac gattcaaacg gcattctcga ttgctttgct
      721 atcgaaggaa agccggatgc ggttgaaact atagcaaatg cttacgtgaa gctcggtcgc
      781 catcgagaag gtgtcgtggg ctttgctcag tgctacctgt tcgacgcgca ggacatcgtg
      841 acgttcggcg tcacctatct tgagaagcat ttcggaacca ctccgatcgt gcctccgcac
      901 gaggccgtcg agcgctcttg cgagccttca ggttagaggc cgtcgacaat gataatctgg
      961 atcaacggac ctttcggcgc cggaaagacg acgctcgcta agcggctgcg cgatcggcgt
     1021 tccaaatcgc tgatctttga ccccgaggaa atcgggttcg tggtgaaaga aacggtcccc
     1081 atgccagcga gcggagacta tcaggatctc cccttgtgga ggggacttac gatcgcggcg
     1141 gtcagggaga ttcgaaggaa ttactcgcag gacatcatca tcccaatgac gctcgtgcac
     1201 ccggactatc tgactgagat actcgacggg gtaaggcgga tcgacgatca gctgctgcac
     1261 atctttctga cgctcaacga ggacctattg cgtcaccgga tcgcgaacca gaccatgcat
     1321 cctgacccga atcgaaatgc ggagattcga gagtggcgat tagcgaatgt cgcccgatgc
     1381 ttggccgcaa gggaacggct tccatgcaca acccgtgttc tcgatagtgg tgcacacacc
     1441 agcgatgaac tcgcagcgat ggtgctcgac ggaatcgatg ggcgcacctg atcgccttcg
     1501 acgcctgcgc aaagcgtagc gcgagggtgg cgggctcacg accaaacgcc cagaggtcga
     1561 tcatcgcagg gatgtttggc tttgtggtgc ggacgacggg actcgaaccc gtactctcac
     1621 agagaagcag attttcgtac cacctcgact ttcgccgccg tctgatgacg ttcgtggtct
     1681 ggactgtccc ttcgccattg cccgaaggct ttaggcgccg cccgtccagt ctctacacct
     1741 tcccccgaag gggcttggct cgggattggc ttagggtatt gcccgttagc gttccccgac
     1801 tttgagcggt tctactccgc ggatttcccc gcgggcactc caattttaaa gtctgctgcg
     1861 tctaccgatt tcgccacgtc cgcctttttt cgccgttcct agcgctcgtg cgatgcacct
     1921 atgttgcacc tagcgccgaa tcgttcttcg tcatcctgaa aaaccacgtc tcctaaagcc
     1981 ttgcatagct tatcttttct ccaccacgaa cttttttgtg ggatggtaga aaaaaagact
     2041 ttttaagtcc gctggcttgc caggccttgt tagcttgtac ggtcatggtt atcgggtaaa
     2101 gaatattgac ggcatcgctg gtgtcggtgg ctgaaaagcc ggctcccatc agggcaatag
     2161 ccatttcaga tgcaggcgta cagggcaatg gtcaacagct acagcctgtc tgacgattcc
     2221 ggcgtcatgg ctgcggcggc tatcacgcat tttttgttcg gtcaggcggt gttttcgtac
     2281 ctcaatggtt ggagcgtgtt gatcggacct ggtacaggtt tggacagcac gggctgcaaa
     2341 tacgcaaggg atttaatggg cctggtggcg ttcacggctt ttatcgtgac gtttctgttc
     2401 aggggctact cataatctcg tggctcggcg gttcccggca caccatgaca gtaaggaagg
     2461 accctgtgtc tcaactctcc cagcttcgaa gccccgccgc cgtgcaggct gccatcgatg
     2521 agttcgtgca actgggccgc acgaaattcc tggcgcgcca cggctacggc aagtcccgcg
     2581 acttcctggt acgtgatccg aagaccggca ccgattgcga ttccaaggcc atcgccggtg
     2641 tggccttcgg caagcaattt cccgagcagg gcccgctcac tgctgacagc ttctccggtg
     2701 gcgaggcgac cgtcgttccg gcgctgacgc ggctcgggtt tcgcatcatt cgcatcggcg
     2761 aagactggtc cgaagaagag gtcctggcca cggtcgaaga ctatttcgac atgctgcgtg
     2821 ccgaggcggc tggggagccg tacaacaagt ccgagcacaa ccaggcactg cgccaactgc
     2881 tgaacggtcg cagcaagtct tcagtcgagc tcaagcacca gaacattagc gccgtactcg
     2941 atgccctggg cctgccctat atcaacggct acaagccacg cggcaacagc caactgctgc
     3001 tgcgtaaatc cgtacacgcc tacgttctgg aacatcagca gacggtcggc gctcttgtcg
     3061 atgccctgga ggaggtaaaa cttccgggtg acaaaaccta ccgagcggct ttggtagaac
     3121 cacccgcccg tgaagtgctt gtgcgtaccc cggcatctct acggcaacgc ctaccgcgaa
     3181 agttcgatta tgccgctcgc gatgaagcca accgcaagct gggccgggca ggggagcagt
     3241 gggtgattgg ctacgaacag caacgcctga ccgagctcgg ccacccagag ctttttcagc
     3301 ggctggattg ggtgtccgac acccagggag acggtgcggg gttcgacatc ctgtcgttcg
     3361 aagaggacgc ccatgagcgc ttcatcgagg tgaaaaccac caatggcggg gtaggctcgt
     3421 ctttcttggt cagccacaac gaactcgaat tctccaagga ggcgggcgat caattccatc
     3481 tgtatcgcgt gttccagttt cgggacggtc cgcgcctgtt cacgctaccc ggcgacctca
     3541 gccaacatgt gcatctcaag ccgacgggca ctgttgcaaa tagtcggtgg tgataaactt
     3601 atcatc
//
LOCUS       DAJOTB010000425         2585 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.298, whole genome shotgun sequence.
ACCESSION   DAJOTB010000425 DAJOTB010000000
VERSION     DAJOTB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.298"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            366..932
                     /locus_tag="OHN28_005094"
     CDS             366..932
                     /locus_tag="OHN28_005094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HCQ2300319.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVDANSSSLLIG
                     ENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLGRDAGQAESK
                     YGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVSVGAGPEGYG
                     ESVKGAPETHSIVNKTFLSFIFNGLFSC"
     gene            1173..1988
                     /gene="sul2"
                     /locus_tag="OHN28_005095"
     CDS             1173..1988
                     /gene="sul2"
                     /locus_tag="OHN28_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCQ2300320.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2075..2377
                     /locus_tag="OHN28_005096"
     CDS             2075..2377
                     /locus_tag="OHN28_005096"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2300321.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
BASE COUNT          576 a          671 c          666 g          672 t
ORIGIN      
        1 tccatgcctg ccacatgtac gtctgcattc ctcgttttga acagaattct caccagacat
       61 cgtgaatttc tcattcaatc atcagagata ttaaccagga tttttattgt tttgtgatat
      121 cagcagtgca ggtatacaga cgttaaagta atcaggttta ttataaccac ctttacaaag
      181 ttgtatatgt ttataattta atgattttaa tgccagtcgt tttctgctgc ttaaattgag
      241 gtcgcatatt atacacaata atattcctgg cattattaaa ttaataaaag aggattctta
      301 tcactgtatt tttgctccgc actaccgttc tggctggtga agtaataata caggagaata
      361 acagaatgct taaaagacgt gatgcttttt taaagaaaag cgcgctggca gtgagtgtgg
      421 ccctgctgct ctcggctcag gctcaggctg ttttgacagg gcctgttgat gctaattctt
      481 ccagcttact gattggagaa aactctttta ttacgaattc aactggtaca gctaataaca
      541 ctttcctttt gggaggtggc gcttttaaca tggattcacc aggaagtctt cagtttggct
      601 ctttttcggg agtgtataat tctccacaca gtgtcaccct gggaagggat gccggtcagg
      661 ctgagtcaaa atatggtgtt gccataggta agagcgctga ggtacttaat tcacagcaga
      721 gtgttgctat tgggggatgg gctggaatag aaaattcttc aggctctgta gctctgggcc
      781 atggctcaca ggtgtcggga gaaaataacg tggtatctgt tggtgccggg cctgagggat
      841 atggagagag tgtcaaagga gcaccggaaa cccactctat tgtaaacaag acatttttat
      901 cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa ataccatgct
      961 cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc acagctccat
     1021 aggccgcttt cctggctttg cttccagatg tatgctattc tgctcctgca gctaatggat
     1081 caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgagact
     1141 catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca
     1201 tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag
     1261 ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg
     1321 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc
     1381 cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg
     1441 cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc
     1501 cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt
     1561 cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca
     1621 ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg agccggtatc aaacgcaacc
     1681 gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct
     1741 cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg
     1801 tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg
     1861 cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg
     1921 agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa
     1981 ttcgttaact gcacattcgg gatatttctc tatattcgcg gttcagcagg catgtcccct
     2041 ttgagggcga cccgacgaca ggataatcga ccttatggtg cgcaaatatt tcggcacaga
     2101 cggtattcgt ggcaaagcca acgaaggcgc gatgacggcg gaaaccgcct tgcgcgtcgg
     2161 catggcggct ggccgtgtct ttcgtcgcgg tgaccaccgc catcgtgtcg tgatcggcaa
     2221 ggatacgcgc ctgtcgggct atatgcttga acccgcgctc acagccggtt tcacctcgat
     2281 gggcatggac gtattccttt ttggcccgct gccgacaacg tataggaaga ataaacgccc
     2341 ttttcaccca agtccaacag ctttggaccg cagttgactc tttcgacacc cctgcgatgc
     2401 aacccaatcc ggctgacggg gagccagcaa cgctgaaaat ttaccctcct ctttcccact
     2461 agcggctcct tttccgacaa ccagcacggc ggatccctgc cgcggcgctg tgaacgcagc
     2521 attttgattg gtatcgttgg ccttcaggct cgtcagtcaa acagacccag gagcagctca
     2581 gccgg
//
LOCUS       DAJOTB010000426         2079 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.311, whole genome shotgun sequence.
ACCESSION   DAJOTB010000426 DAJOTB010000000
VERSION     DAJOTB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.311"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..951
                     /locus_tag="OHN28_005097"
     CDS             163..951
                     /locus_tag="OHN28_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCQ2300322.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1157..1504
                     /locus_tag="OHN28_005098"
     CDS             1157..1504
                     /locus_tag="OHN28_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCQ2300323.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1498..>2079
                     /gene="folP"
                     /locus_tag="OHN28_005099"
     CDS             1498..>2079
                     /gene="folP"
                     /locus_tag="OHN28_005099"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCQ2300324.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNL"
BASE COUNT          416 a          587 c          583 g          493 t
ORIGIN      
        1 attatacata ccagatttgg aaaaaaggct aacaatgcgt tgcagcacca gtcgcttcgc
       61 tccttggaca gcttttaagt cgcgtctttg tggttttgct gcgcaaaagt attccacaaa
      121 gccgcaactt aaaagctgcc gctgaactta acgttaggca tcatgggtga atttttccct
      181 gcacaagttt tcaagcagct gtcccacgct cgcgcggtga tcgagcgcca tctggctgcg
      241 acactggaca caatccacct gttcggatct gcgatcgatg gagggctgaa gccggacagc
      301 gacatagact tgctcgtgac cgtcagcgcc gcacctaacg attcgctccg gcaggcgcta
      361 atgctcgatt tgctgaaagt ctcatcaccg ccaggcgatg gcggaacatg gcgaccgctg
      421 gagctaactg ttgtcgctcg aagcgaagta gtgccttggc gctatccggc gcggcgtgag
      481 cttcagttcg gtgagtggct ccgccacgac atcctttccg gaacgttcga gcctgccgtt
      541 ctggatcacg atcttgcgat tttgctgacc aaggcgaggc aacacagcct tgcgcttcta
      601 ggcccatccg cagccacgtt tttcgagccg gtgccgaagg agcatttctc caaggcgctt
      661 ttcgacacta ttgcccagtg gaatgcagag tcggattgga agggtgacga gcggaacgtc
      721 gttcttgctc ttgctcgcat ttggtacagc gcttcaactg gtctcattgc tcctaaggac
      781 gttgctgccg catgggtatc ggagcgtttg cctgccgagc atcggcccct catctgcaag
      841 gcacgcgcgg cgtacctggg tagcgaggac gacgacctag caatgcgcgt cgaagagacg
      901 gccgcgttcg ttcgatatgc caaagcaacg attgagagaa tcttgcgttg agcggcatgt
      961 gcgaaaagtg catcgacccg cgccgagggc atctgatgcc taactcggcg ttcaagcgga
     1021 cgggctgcgc ccgccgctca actatgcgtt agatgcacta agcacataat tgctcacagc
     1081 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     1141 aattgggaga tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt
     1201 aatcgcaaca tccgcattaa aatctagcga gggctttact aagcttgccc cttccgccgt
     1261 tgtcataatc ggttatggca tcgcatttta ttttctttct ctggttctga aatccatccc
     1321 tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc gtcataatta cagccattgc
     1381 ctggttgctt catgggcaaa agcttgatgc gtggggcttt gtaggtatgg ggctcataat
     1441 tgctgccttt ttgctcgccc gatccccatc gtggaagtcg ctgcggaggc cgacgccatg
     1501 gtgacggtgt tcggcattct gaatctcacc gaggactcct tcttcgatga gagccggcgg
     1561 ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc tgcgagtcgg atcagacgtc
     1621 gtggatgtcg gaccggccgc cagccatccg gacgcgaggc ctgtatcgcc ggccgatgag
     1681 atcagacgta ttgcgccgct cttagacgcc ctgtccgatc agatgcaccg tgtttcaatc
     1741 gacagcttcc aaccggaaac ccagcgctat gcgctcaagc gcggcgtggg ctacctgaac
     1801 gatatccaag gatttcctga ccctgcgctc tatcccgata ttgctgaggc ggactgcagg
     1861 ctggtggtta tgcactcagc gcagcgggat ggcatcgcca cccgcaccgg tcaccttcga
     1921 cccgaagacg cgctcgacga gattgtgcgg ttcttcgagg cgcgggtttc cgccttgcga
     1981 cggagcgggg tcgctgccga ccggctcatc ctcgatccgg ggatgggatt tttcttgagc
     2041 cccgcaccgg aaacatcgct gcacgtgctg tcgaacctt
//
LOCUS       DAJOTB010000427         2018 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.314, whole genome shotgun sequence.
ACCESSION   DAJOTB010000427 DAJOTB010000000
VERSION     DAJOTB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.314"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            21..836
                     /locus_tag="OHN28_005100"
     CDS             21..836
                     /locus_tag="OHN28_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2300325.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(994..>2018)
                     /locus_tag="OHN28_005101"
     CDS             complement(994..>2018)
                     /locus_tag="OHN28_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2300326.1"
                     /translation="THTFWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGT
                     SASSDGQFFVATEQGEAMNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPY
                     ILDGLLMNDAGRHIREQFTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSA
                     APAHLRALIGGKVNQAMIERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATA
                     LREVGRVERTLFMIDWILDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEG
                     QHYRIAGMNLLAAIIIFWNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGE
                     YRWPKP"
BASE COUNT          437 a          495 c          585 g          501 t
ORIGIN      
        1 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc
       61 gattaaattc caacctggat gctgatttat atgggtataa atgggctcgc gataatgtcg
      121 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
      181 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
      241 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
      301 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
      361 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
      421 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
      481 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
      541 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
      601 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
      661 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
      721 actgcctcgg tgaattttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
      781 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctgatagt
      841 tagtctttgt agagacacaa acctgaaatt ccggacaccg cgcttcagga catcgctcaa
      901 gacgcctata tcgggcagta tccggacggt actatcgaac gccggtaccc tgccctttat
      961 ggggtcgttt gcgggagggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     1021 ggtgagattg atgtgttccc atccgagcgg cgaaacatgg gccaagagat cgggcgatag
     1081 cagctttcca tcgcgtttct ggtttgcaac gacctcgccg agcttcatgg tgttccagaa
     1141 gatgatgatg gcggcgagca gattcatgcc ggcgatgcgg taatgctggc cttcggcgga
     1201 acggtcgcgg atttcaccgc ggcggtggaa gctgattgcc cgcttcagcg catgatgagc
     1261 ttcgcctttg ttgagcccga tctgggcacg ccgttggagt tcggcatcca gaatccagtc
     1321 gatcatgaac agggtgcgct cgacgcgacc gacttcccgc agggctgtcg cgagctcgtt
     1381 ctgccgcgga taggaggcga gtttccgcag aatctggctt ggcgcgacgg tcccggcagc
     1441 aatggtggcg gcgatgcgca ggatgtcggg ccaattgcgc tcgatcatgg cttggttgac
     1501 ctttccgccg atcaacgctc gcaggtgcgc cggggcggcc gacggattga acgcgtagag
     1561 ccgtttggat ggcaggtcgc ggatgcgcgg agcgaaccgg tagccgagaa tggcacatgc
     1621 ggcaaagacg tgatcggtga agccgcccgt gtcggtgaac tgctcgcgga tatggcgtcc
     1681 agcatcgttc atcagcaggc catcgaggat gtaaggcgct tcgcttgccg ttgcaggaat
     1741 cacctgggtt gcgaacggcg catattggtc ggagacgtgg ctataggctt tcaggcccgg
     1801 ggtattgcca tatttcgcgt tgaccaggtt catggcctca ccttgctctg tagcgacgaa
     1861 gaactgtccg tcgctcgaag ccgacgtgcc catgccccag aaccgggcca tgggtaacgc
     1921 tgcctgtgcc tcgaccacca tggccagcgc ccggtcatag gcttcgccct cgacatgcca
     1981 ccgtccaatg cggatcaatt cccagaaggt gtgggtgt
//
LOCUS       DAJOTB010000428         1864 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.320, whole genome shotgun sequence.
ACCESSION   DAJOTB010000428 DAJOTB010000000
VERSION     DAJOTB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.320"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            29..832
                     /locus_tag="OHN28_005102"
                     /note="aph(3'')-Ib"
     CDS             29..832
                     /locus_tag="OHN28_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCQ2300327.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            832..1668
                     /locus_tag="OHN28_005103"
     CDS             832..1668
                     /locus_tag="OHN28_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2300328.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYAHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          401 a          495 c          523 g          445 t
ORIGIN      
        1 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
       61 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
      121 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
      181 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tgatcaactg
      241 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
      301 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
      361 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
      421 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
      481 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
      541 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
      601 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
      661 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
      721 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
      781 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
      841 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
      901 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
      961 aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     1021 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     1081 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     1141 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     1201 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     1261 ggttgtcaaa ctgactacgc ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     1321 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     1381 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     1441 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     1501 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     1561 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     1621 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     1681 ttctcctatc ccctgggaac acatcaatct taccggagaa tatcgttggc caaagcctta
     1741 gcgtaggatt tcgccctctc ccgcaaacga ccccgggaac cacgttgtgt ctcaaaatct
     1801 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1861 cata
//
LOCUS       DAJOTB010000429         1864 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.321, whole genome shotgun sequence.
ACCESSION   DAJOTB010000429 DAJOTB010000000
VERSION     DAJOTB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.321"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            29..832
                     /locus_tag="OHN28_005104"
                     /note="aph(3'')-Ib"
     CDS             29..832
                     /locus_tag="OHN28_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCQ2300329.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            832..1668
                     /locus_tag="OHN28_005105"
     CDS             832..1668
                     /locus_tag="OHN28_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCQ2300330.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          401 a          494 c          523 g          446 t
ORIGIN      
        1 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
       61 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
      121 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
      181 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tgatcaactg
      241 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
      301 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
      361 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
      421 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
      481 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
      541 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
      601 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
      661 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
      721 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
      781 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
      841 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
      901 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
      961 aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     1021 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     1081 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     1141 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     1201 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     1261 ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     1321 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     1381 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     1441 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     1501 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     1561 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     1621 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     1681 ttctcctatc ccctgggaac acatcaatct taccggagaa tatcgttggc caaagcctta
     1741 gcgtaggatt tcgccctctc ccgcaaacga ccccgggaac cacgttgtgt ctcaaaatct
     1801 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1861 cata
//
LOCUS       DAJOTB010000430         1800 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.326, whole genome shotgun sequence.
ACCESSION   DAJOTB010000430 DAJOTB010000000
VERSION     DAJOTB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.326"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            41..1174
                     /locus_tag="OHN28_005106"
     CDS             41..1174
                     /locus_tag="OHN28_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2300331.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            1263..1796
                     /gene="blc"
                     /locus_tag="OHN28_005107"
     CDS             1263..1796
                     /gene="blc"
                     /locus_tag="OHN28_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCQ2300332.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
BASE COUNT          463 a          463 c          447 g          427 t
ORIGIN      
        1 cccgcctatg gcgggccgtt ttgtatggaa accagaccct atgttcaaaa cgacgctctg
       61 caccttatta attaccgcct cttgctccac atttgccgcc cctcaacaaa tcaacgatat
      121 tgtgcatcgc acaattaccc cgcttataga gcaacaaagg atccccggta tggcggtagc
      181 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
      241 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
      301 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa
      361 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac
      421 ctacaccgct ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt
      481 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
      541 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
      601 gcaggcgatg aaaactcgtg tcttccagcc actcaaactc aatcatacgt ggattaatgt
      661 accgtccgca gaagaaaaga actacgcctg gggatatcgc gaaggtaagg cagtacatgt
      721 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
      781 ccgctgggtg caaagcaatt taaaacccct tgatatcaat gagaaaatac ttcaacaagg
      841 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gtctgggctg
      901 ggaaatgctg gactggccgg taaatcctga catcatcatt aacggcagtg acaataaaat
      961 tgcactggca gcacgccccg taaaaccgat tacgccccca actcctgcag tacgcgcatc
     1021 atgggtacat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga
     1081 aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagttac
     1141 cgccgcctgg cagattctca acactctaca gtaaaatttc atcgggtccg aattttcgga
     1201 ccttttctcc gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat
     1261 ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca
     1321 gttctcctac accgccgcgt ggcgtgaccg tagtgaataa tttcgacgct aaacgctatc
     1381 tcggcacctg gtatgagatt gcccgttttg atcaccgctt tgaacgtgga ctggaaaaag
     1441 tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata
     1501 accctgacag agaaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa
     1561 ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg
     1621 cactcgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga
     1681 tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga
     1741 cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttaatgag
//
LOCUS       DAJOTB010000431         1627 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.332, whole genome shotgun sequence.
ACCESSION   DAJOTB010000431 DAJOTB010000000
VERSION     DAJOTB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.332"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..437
                     /locus_tag="OHN28_005108"
     CDS             <1..437
                     /locus_tag="OHN28_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2300333.1"
                     /translation="STDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDA
                     QGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRR
                     RTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            620..1480
                     /locus_tag="OHN28_005109"
     CDS             620..1480
                     /locus_tag="OHN28_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCQ2300334.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          436 a          356 c          416 g          419 t
ORIGIN      
        1 gttcaacaga ccgggaaggg ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca
       61 ttctggttaa gaagctcgac cgtcttggcc gcgacactgc cgatatgatc caactgataa
      121 aggaatttga cgctcagggc gtggcagtcc ggttcattga tgacgggatc agtaccgacg
      181 gtgatatggg gcaaatggtg gtcaccatcc tgtcggctgt ggcacaggct gaacgccgga
      241 ggatcctaga acgcacgaat gagggccgac aggaagcaaa gctgaaagga atcaaatttg
      301 gccgcaggcg taccgtggac aggaacgtcg tgctgacgct tcatcagaag ggcactggtg
      361 caacggaaat tgctcatcag ctcagtattg cccgctccac ggtttataaa attcttgaag
      421 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      481 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctggtaaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acattttcgt gtcgccctta ttcccttttt
      661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      841 atgtggtgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgctgccaa
     1021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1141 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1621 agacccc
//
LOCUS       DAJOTB010000432         1319 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.351, whole genome shotgun sequence.
ACCESSION   DAJOTB010000432 DAJOTB010000000
VERSION     DAJOTB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.351"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            69..1274
                     /gene="tet(B)"
                     /locus_tag="OHN28_005110"
     CDS             69..1274
                     /gene="tet(B)"
                     /locus_tag="OHN28_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCQ2300335.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          307 a          246 c          315 g          451 t
ORIGIN      
        1 tttttgttga cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga
       61 aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg
      121 ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag
      181 atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg
      241 ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat
      301 taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt
      361 atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca
      421 ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt
      481 ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc
      541 atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg gttatgtttt
      601 ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc
      661 aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt
      721 tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc
      781 gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact
      841 cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag
      901 tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt atatctgaag
      961 gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat
     1021 tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat
     1081 tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata
     1141 atcattcact accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta
     1201 ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg
     1261 agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccc
//
LOCUS       DAJOTB010000433         1319 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.352, whole genome shotgun sequence.
ACCESSION   DAJOTB010000433 DAJOTB010000000
VERSION     DAJOTB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.352"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            69..1274
                     /gene="tet(B)"
                     /locus_tag="OHN28_005111"
     CDS             69..1274
                     /gene="tet(B)"
                     /locus_tag="OHN28_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCQ2300336.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIAVSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          306 a          246 c          315 g          452 t
ORIGIN      
        1 tttttgttga cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga
       61 aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg
      121 ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag
      181 atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg
      241 ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat
      301 taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt
      361 atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca
      421 ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt
      481 ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc
      541 atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg gttatgtttt
      601 ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc
      661 aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt
      721 tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc
      781 gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact
      841 cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag
      901 tactgctcgg atttattgca gttagtagtg catttgcctt tttagcgttt atatctgaag
      961 gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat
     1021 tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat
     1081 tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata
     1141 atcattcact accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta
     1201 ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg
     1261 agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccc
//
LOCUS       DAJOTB010000434         1055 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.367, whole genome shotgun sequence.
ACCESSION   DAJOTB010000434 DAJOTB010000000
VERSION     DAJOTB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.367"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..121
                     /locus_tag="OHN28_005112"
     CDS             <1..121
                     /locus_tag="OHN28_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HCQ2300337.1"
                     /translation="LLHGQKLDAWGFVGMGLIIAAFLLARSPSWKSLRRPTPW"
     gene            115..954
                     /gene="sul1"
                     /locus_tag="OHN28_005113"
     CDS             115..954
                     /gene="sul1"
                     /locus_tag="OHN28_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCQ2300338.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
BASE COUNT          187 a          324 c          325 g          219 t
ORIGIN      
        1 gttgcttcat gggcaaaagc ttgatgcgtg gggctttgta ggtatggggc tcataattgc
       61 tgcctttttg ctcgcccgat ccccatcgtg gaagtcgctg cggaggccga cgccatggtg
      121 acggtgttcg gcattctgaa tctcaccgag gactccttct tcgatgagag ccggcggcta
      181 gaccccgccg gcgctgtcac cgcggcgatc gaaatgctgc gagtcggatc agacgtcgtg
      241 gatgtcggac cggccgccag ccatccggac gcgaggcctg tatcgccggc cgatgagatc
      301 agacgtattg cgccgctctt agacgccctg tccgatcaga tgcaccgtgt ttcaatcgac
      361 agcttccaac cggaaaccca gcgctatgcg ctcaagcgcg gcgtgggcta cctgaacgat
      421 atccaaggat ttcctgaccc tgcgctctat cccgatattg ctgaggcgga ctgcaggctg
      481 gtggttatgc actcagcgca gcgggatggc atcgccaccc gcaccggtca ccttcgaccc
      541 gaagacgcgc tcgacgagat tgtgcggttc ttcgaggcgc gggtttccgc cttgcgacgg
      601 agcggggtcg ctgccgaccg gctcatcctc gatccgggga tgggattttt cttgagcccc
      661 gcaccggaaa catcgctgca cgtgctgtcg aaccttcaaa agctgaagtc ggcgttgggg
      721 cttccgctat tggtctcggt gtcgcggaaa tccttcttgg gcgccaccgt tggccttcct
      781 gtaaaggatc tgggtccagc gagccttgcg gcggaacttc acgcgatcgg caatggcgct
      841 gactacgtcc gcacccacgc gcctggagat ctgcgaagcg caatcacctt ctcggaaacc
      901 ctcgcgaaat ttcgcagtcg cgacgccaga gaccgagggt tagatcatgc ctagcattca
      961 ccttccggcc gcccgctagc ggaccctggt caggttccgc gaaggtgggc gcagacatgc
     1021 tgggctcgtc aggatcaaac tgcactatga ggcgg
//
LOCUS       DAJOTB010000435          740 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP214
            SAMN25142496-rid15605953.guided.392, whole genome shotgun sequence.
ACCESSION   DAJOTB010000435 DAJOTB010000000
VERSION     DAJOTB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142496
            Sequence Read Archive: SRR17680999
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453566.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453566.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 18:45:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,887
            CDSs (with protein)               :: 4,887
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 5, 1 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 72 of 142
            Pseudo Genes (incomplete)         :: 85 of 142
            Pseudo Genes (internal stop)      :: 24 of 142
            Pseudo Genes (multiple problems)  :: 36 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142496-rid15605953.guided.392"
                     /isolate="PP214"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..148)
                     /locus_tag="OHN28_005114"
     CDS             complement(<1..148)
                     /locus_tag="OHN28_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCQ2300339.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHG"
     gene            306..>740
                     /gene="dfrA"
                     /locus_tag="OHN28_005115"
     CDS             306..>740
                     /gene="dfrA"
                     /locus_tag="OHN28_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCQ2300340.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMS"
BASE COUNT          214 a          142 c          173 g          211 t
ORIGIN      
        1 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaaac
       61 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      121 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta
      181 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      241 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttagcc attaagggag
      301 ttaaattgaa aatatcattg atttctgcag tgtcagaaaa tggcgtaatc ggtagtggtc
      361 ctgatatccc gtggtcagta aaaggtgagc aactactctt taaagcgctc acatataatc
      421 aatggctcct tgtcggaaga aaaacatttg actctatggg tgttcttcca aatcgcaaat
      481 atgcagtagt gtcaaagaac ggaatttcaa gctcaaatga aaacgtccta gtttttcctt
      541 caatagaaaa tgctttgaaa gagctatcaa aagttacaga tcatgtatat gtctctggcg
      601 ggggtcaaat ctataatagc cttattgaaa aagcagatat aattcatttg tctactgttc
      661 acgttgaagt cgaaggtgat atcaaattcc ctataatgcc tgagaatttc aatttggttt
      721 ttgaacagtt ttttatgtct
//
